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Sample records for shoshone county id

  1. 76 FR 22076 - Bussel 484, Idaho Panhandle National Forests, Idaho, Shoshone County

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-20

    ... of wildife, the Idaho Panhandle National Forests' fire management direction, recently designated... Forest Service Bussel 484, Idaho Panhandle National Forests, Idaho, Shoshone County AGENCY: Forest...: The USDA Forest Service will prepare a supplemental environmental impact statement (SEIS) for...

  2. 78 FR 35602 - Coeur d'Alene Basin Restoration Plan, Kootenai, Shoshone and Benewah Counties, Idaho

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-13

    ... Wildlife Service Coeur d'Alene Basin Restoration Plan, Kootenai, Shoshone and Benewah Counties, Idaho... Department of Agriculture (USDA), Coeur d'Alene Indian Tribe (Tribe), and the State of Idaho (State) intend... related to the Coeur d'Alene Basin Restoration Plan by any of the following methods: Web site:...

  3. North Fork well, Shoshone National Forest, Park County, Wyoming

    SciTech Connect

    Not Available

    1984-01-01

    A summary of the draft environmental impact statement for a proposed exploratory oil drilling operation in Shoshone National Forest in Wyoming describes the drilling equipment and support facilities required for the operation. Marathon Oil Company's purpose is to test the gas and oil potential of underlying geologic structures. Although Marathon plans a reclamation and revegetation program, there would be erosion during the operation. Noise from the drilling and helicopter activity would disrupt wildlife and vacationers in nearby Yellowstone Park. Confrontations with the grizzly bear population would increase. The legal mandate for the assessment was the Mineral Leasing Act of 1920.

  4. 75 FR 50777 - Minidoka Dam Spillway Replacement, Minidoka County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-17

    ... on December 11, 2009 (74 FR 65783) with a public comment period ending on February 5, 2010. The Final... Bureau of Reclamation Minidoka Dam Spillway Replacement, Minidoka County, ID AGENCY: Bureau of... proposed Minidoka Dam Spillway Replacement. The U.S. Fish and Wildlife Service (Department of Interior)...

  5. 7. SHOSHONE HYDROELECTRIC PLANT, WEST ELEVATION OF MAIN BUILDING TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. SHOSHONE HYDROELECTRIC PLANT, WEST ELEVATION OF MAIN BUILDING TO THE LEFT, NORTH ELEVATION OF OFFICE BUILDING TO THE RIGHT, VIEW TO THE EAST. CONCRETE 'PATH' IN FOREGROUND IS THE CONDUIT THROUGH WHICH POWER CABLES RUN FROM THE TRANSFORMERS TO THE 115 KV SUBSTATION. - Shoshone Hydroelectric Plant Complex, 60111 U.S. Highway 6, Garfield County, CO

  6. 11. SHOSHONE INTAKE DAM, VIEW TO THE NORTHWEST. RESTROOM BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. SHOSHONE INTAKE DAM, VIEW TO THE NORTHWEST. RESTROOM BUILDING AND STEAM CLEANER BUILDING ARE SEEN BELOW THE WEST SPAN OF THE BRIDGE; HOIST HOUSE AND CABLEWAY TOWER APE ABOVE CENTER. - Shoshone Hydroelectric Plant Complex, 60111 U.S. Highway 6, Garfield County, CO

  7. 76 FR 11264 - Notice of Realty Action: Proposed Direct Sale of Public Land in Shoshone County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-01

    ...: Written comments concerning this proposed sale may be submitted to Field Manager, BLM Coeur d'Alene Field Office, 3815 Schreiber Way, Coeur d'Alene, Idaho 83815. FOR FURTHER INFORMATION CONTACT: Janna Paronto, Realty Specialist, at 3815 Schreiber Way, Coeur d'Alene, Idaho 83815 or phone (208)...

  8. 78 FR 4377 - Idaho Panhandle National Forests, Coeur d'Alene River Ranger District, Shoshone County, ID...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ... proposal to accomplish vegetation management in the Beaver Creek Resource Area, which is located on... identified by the project team include (1) effects of the proposed activities on forest vegetation;...

  9. 77 FR 56607 - Shoshone Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ... Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee) is holding a meeting on September... INFORMATION CONTACT: Olga Troxel, Resource Advisory Committee Coordinator, Shoshone National Forest...

  10. 76 FR 41196 - Shoshone Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-13

    ... Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee) will meet in Thermopolis, Wyoming...: Olga Troxel, Resource Advisory Committee Coordinator, Shoshone National Forest Supervisor's...

  11. 76 FR 22672 - Shoshone Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-22

    ... Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee) will meet in Thermopolis, Wyoming... CONTACT: Olga Troxel, Resource Advisory Committee Coordinator, Shoshone National Forest...

  12. 77 FR 55800 - Foreign-Trade Zone 242-Boundary County, ID; Application for Subzone AREVA Enrichment Services...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 242--Boundary County, ID; Application for Subzone AREVA Enrichment Services, LLC; Bonneville County, ID An application has been submitted to the Foreign-Trade...

  13. 75 FR 35083 - Notice of Realty Action; Direct Sale of Public Lands in Lincoln County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Land Management Notice of Realty Action; Direct Sale of Public Lands in Lincoln County, ID AGENCY: Bureau of Land Management, Interior. ACTION: Notice of realty action. SUMMARY: The Bureau of...

  14. 77 FR 13072 - Salmon-Challis National Forest, Butte, Custer and Lemhi Counties, ID, Supplemental Environmental...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... Forest Service Salmon-Challis National Forest, Butte, Custer and Lemhi Counties, ID, Supplemental Environmental Impact Statement to the 2009 Salmon- Challis National Forest Travel Planning and OHV Route... environmental impact statement. SUMMARY: The Salmon-Challis National Forest announces its intent to prepare...

  15. 75 FR 45661 - Notice of Permanent Closure on Public Lands in Ada County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... Bureau of Land Management Notice of Permanent Closure on Public Lands in Ada County, ID AGENCY: Bureau of Land Management, Interior. ACTION: Notice of permanent closure. SUMMARY: On April 12, 2010, Higby Cave and all public lands within 1,000 feet of the entrance were permanently closed to vehicle access...

  16. 78 FR 39767 - Notice of Realty Action: Direct Sale of Public Land in Blaine County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... sale. The BLM has completed a mineral potential report which concluded there ] are no known mineral values in the lands proposed for sale. The BLM proposes that conveyance of the Federal mineral interests... Bureau of Land Management Notice of Realty Action: Direct Sale of Public Land in Blaine County, ID...

  17. Modeling Approach/Strategy for Corrective Action Unit 99: Rainier Mesa and Shoshone Mountain, Nevada Test Site, Nye County, Nevada, Revision 1, with ROTC-1

    SciTech Connect

    Greg Ruskauff

    2008-06-01

    This document describes an approach for preliminary (Phase I) flow and transport modeling for the Rainier Mesa/Shoshone Mountain (RMSM) Corrective Action Unit (CAU). This modeling will take place before the planned Phase II round of data collection to better identify the remaining data gaps before the fieldwork begins. Because of the geologic complexity, limited number of borings, and large vertical gradients, there is considerable uncertainty in the conceptual model for flow; thus different conceptual models will be evaluated, in addition to different framework and recharge models. The transport simulations will not be used to formally calculate the Contaminant Boundary at this time. The modeling (Phase II) will occur only after the available data are considered sufficient in scope and quality.

  18. 75 FR 22625 - Notice of Realty Action; Direct Sale of Public Land in Jerome County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-29

    ... FURTHER INFORMATION CONTACT: Lisa Claxton, Natural Resource Specialist, at the above address or phone (208... 1985 BLM Monument Resource Management Plan, as amended by the 2003 Amendments to BLM Shoshone...

  19. 76 FR 12317 - Shoshone Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ... Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee) will meet in Thermopolis, Wyoming... Broadway, Thermopolis, Wyoming. FOR FURTHER INFORMATION CONTACT: Olga Troxel, Resource Advisory...

  20. 76 FR 3081 - Shoshone Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-19

    ... Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee) will hold a conference call on.... FOR FURTHER INFORMATION CONTACT: Olga Troxel, Resource Advisory Committee Coordinator,...

  1. 75 FR 55539 - Shoshone Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee will meet in Thermopolis, Wyoming....

  2. 77 FR 47360 - Shoshone Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ... Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee) will hold a conference call on.... FOR FURTHER INFORMATION CONTACT: Olga Troxel, Resource Advisory Committee Coordinator,...

  3. 75 FR 79018 - Notice of Realty Action: Proposed Sale of Public Lands in Bear Lake County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-17

    ... Bureau of Land Management Notice of Realty Action: Proposed Sale of Public Lands in Bear Lake County, ID... Management (BLM) proposes the sale of 26 parcels of public lands totaling 1,543.14 acres in Bear Lake County... Bear Lake County, Idaho, are proposed for sale under the authority of Sections 203 and 209 of FLPMA...

  4. A Hydrostrat Model and Alternatives for Groundwater Flow and Contaminant Transport Model of Corrective Action Unit 99: Rainer Mesa-Shoshone Mountain, Nye County, Nevada

    SciTech Connect

    NSTec Geotechnical Sciences Group

    2007-03-01

    The three-dimensional hydrostratigraphic framework model for the Rainier Mesa-Shoshone Mountain Corrective Action Unit was completed in Fiscal Year 2006. The model extends from eastern Pahute Mesa in the north to Mid Valley in the south and centers on the former nuclear testing areas at Rainier Mesa, Aqueduct Mesa, and Shoshone Mountain. The model area also includes an overlap with the existing Underground Test Area Corrective Action Unit models for Yucca Flat and Pahute Mesa. The model area is geologically diverse and includes un-extended yet highly deformed Paleozoic terrain and high volcanic mesas between the Yucca Flat extensional basin on the east and caldera complexes of the Southwestern Nevada Volcanic Field on the west. The area also includes a hydrologic divide between two groundwater sub-basins of the Death Valley regional flow system. A diverse set of geological and geophysical data collected over the past 50 years was used to develop a structural model and hydrostratigraphic system for the model area. Three deep characterization wells, a magnetotelluric survey, and reprocessed gravity data were acquired specifically for this modeling initiative. These data and associated interpretive products were integrated using EarthVision{reg_sign} software to develop the three-dimensional hydrostratigraphic framework model. Crucial steps in the model building process included establishing a fault model, developing a hydrostratigraphic scheme, compiling a drill-hole database, and constructing detailed geologic and hydrostratigraphic cross sections and subsurface maps. The more than 100 stratigraphic units in the model area were grouped into 43 hydrostratigraphic units based on each unit's propensity toward aquifer or aquitard characteristics. The authors organized the volcanic units in the model area into 35 hydrostratigraphic units that include 16 aquifers, 12 confining units, 2 composite units (a mixture of aquifer and confining units), and 5 intrusive confining

  5. Predevelopment Water-Level Contours for Aquifers in the Rainier Mesa and Shoshone Mountain area of the Nevada Test Site, Nye County, Nevada

    USGS Publications Warehouse

    Fenelon, Joseph M.; Laczniak, Randell J.; Halford, Keith J.

    2008-01-01

    Contaminants introduced into the subsurface of the Nevada Test Site at Rainier Mesa and Shoshone Mountain by underground nuclear testing are of concern to the U.S. Department of Energy and regulators responsible for protecting human health and safety. Although contaminants were introduced into low-permeability rocks above the regional flow system, the potential for contaminant movement away from the underground test areas and into the accessible environment is greatest by ground-water transport. The primary hydrologic control on this transport is evaluated and examined through a series of contour maps developed to represent the water-level distribution within each of the major aquifers underlying the area. Aquifers were identified and their extents delineated by merging and analyzing multiple hydrostratigraphic framework models developed by other investigators from existing geologic information. The contoured water-level distribution in each major aquifer was developed from a detailed evaluation and assessment of available water-level measurements. Multiple spreadsheets that accompany this report provide pertinent water-level and geologic data by well or drill hole. Aquifers are mapped, presented, and discussed in general terms as being one of three aquifer types?volcanic aquifer, upper carbonate aquifer, or lower carbonate aquifer. Each of these aquifer types was subdivided and mapped as independent continuous and isolated aquifers, based on the continuity of its component rock. Ground-water flow directions, as related to the transport of test-generated contaminants, were developed from water-level contours and are presented and discussed for each of the continuous aquifers. Contoured water-level altitudes vary across the study area and range from more than 5,000 feet in the volcanic aquifer beneath a recharge area in the northern part of the study area to less than 2,450 feet in the lower carbonate aquifer in the southern part of the study area. Variations in

  6. Predevelopment Water-Level Contours for Aquifers in the Rainier Mesa and Shoshone Mountain area of the Nevada Test Site, Nye County, Nevada

    SciTech Connect

    Joseph M. Fenelon; Randell J. Laczniak; and Keith J. Halford

    2008-06-24

    Contaminants introduced into the subsurface of the Nevada Test Site at Rainier Mesa and Shoshone Mountain by underground nuclear testing are of concern to the U.S. Department of Energy and regulators responsible for protecting human health and safety. Although contaminants were introduced into low-permeability rocks above the regional flow system, the potential for contaminant movement away from the underground test areas and into the accessible environment is greatest by ground-water transport. The primary hydrologic control on this transport is evaluated and examined through a series of contour maps developed to represent the water-level distribution within each of the major aquifers underlying the area. Aquifers were identified and their extents delineated by merging and analyzing multiple hydrostratigraphic framework models developed by other investigators from existing geologic information. The contoured water-level distribution in each major aquifer was developed from a detailed evaluation and assessment of available water-level measurements. Multiple spreadsheets that accompany this report provide pertinent water-level and geologic data by well or drill hole. Aquifers are mapped, presented, and discussed in general terms as being one of three aquifer types—volcanic aquifer, upper carbonate aquifer, or lower carbonate aquifer. Each of these aquifer types was subdivided and mapped as independent continuous and isolated aquifers, based on the continuity of its component rock. Ground-water flow directions, as related to the transport of test-generated contaminants, were developed from water-level contours and are presented and discussed for each of the continuous aquifers. Contoured water-level altitudes vary across the study area and range from more than 5,000 feet in the volcanic aquifer beneath a recharge area in the northern part of the study area to less than 2,450 feet in the lower carbonate aquifer in the southern part of the study area. Variations in

  7. NEWE: A Western Shoshone History.

    ERIC Educational Resources Information Center

    Inter-Tribal Council of Nevada, Reno.

    One in a series of four histories of native Nevadans, this volume relates the history of the Western Shoshone, or Newe, whose territory included parts of the Great Basin area which extends from southern California to Idaho. Based on the spoken word of tribal elders and research conducted at numerous archives, the history begins with ancient…

  8. Superfund explanation of significant difference for the Record of Decision (EPA Region 10): Bunker Hill Mining and Metallurgical Complex, Smelterville, Shoshone County, ID, April 18, 1998

    SciTech Connect

    1998-09-01

    During the remedial design phase of cleanup, both EPA and DEQ identified revisions appropriate for the remedy identified in the Bunker Hill Mining and Metallurgical Complex Record of Decision (ROD). These revisions are necessary for several reasons. They will ensure that the remedy is cost-effective, maximizes the benefit to the environment, and is responsive to community concerns.

  9. 77 FR 47593 - Shoshone Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-09

    ... Forest Service Shoshone Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Shoshone Resource Advisory Committee (Committee) will hold a meeting on September 19...-6241 to facilitate entry into the building. FOR FURTHER INFORMATION CONTACT: Olga Troxel,...

  10. Phase I Contaminant Transport Parameters for the Groundwater Flow and Contaminant Transport Model of Corrective Action Unit 99: Rainier Mesa/Shoshone Mountain, Nevada Test Site, Nye County, Nevada, Revision 1

    SciTech Connect

    Nathan Bryant

    2008-05-01

    This document presents a summary and framework of available transport data and other information directly relevant to the development of the Rainier Mesa/Shoshone Mountain (RMSM) Corrective Action Unit (CAU) 99 groundwater transport model. Where appropriate, data and information documented elsewhere are briefly summarized with reference to the complete documentation.

  11. Phase I Hydrologic Data for the Groundwater Flow and Contaminant Transport Model of Corrective Action Unit 99: Rainier Mesa/Shoshone Mountain, Nevada Test Site, Nye County, Nevada, Revision 1

    SciTech Connect

    Nathan Bryant

    2008-05-01

    This document presents a summary and framework of the available hydrologic data and other information directly relevant to the development of the Rainier Mesa/Shoshone Mountain (RMSM) Corrective Action Unit (CAU) 99 groundwater flow models. Where appropriate, data and information documented elsewhere are briefly summarized with reference to the complete documentation.

  12. Shoshone Spirituality Archaeological Interpretation in Southeast Idaho

    SciTech Connect

    Dean, P. A.; Marler, Clayton Fay

    2001-03-01

    Tribal people in southeast Idaho sincerely desire that archaeologists include Shoshone concepts of spirituality when investigating archaeological materials and sites. However, most archaeologists and resource managers have little understanding about these concepts and this creates difficulties. We examine two important aspects of the Shoshone soul, Mugua’ and Nabushi’aipe, and discuss how understanding these attributes aid in explaining why certain archaeological remains are considered sacred. A greater understanding of Shoshone spirituality will begin to bridge the needs of both tribal people and archaeologists.

  13. 75 FR 71143 - Notice of Realty Action: Direct Sale of Public Land in Blaine County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... Animal Shelter of Wood River Valley (Animal Shelter) for the appraised fair market value of $18,700.... The Animal Shelter is a non-profit corporation that provides public benefits to Blaine County (County.... The Animal Shelter was issued a right-of-way for a road and a fence that cross the 17-acre...

  14. 76 FR 78940 - Minidoka National Wildlife Refuge, Blaine, Cassia, Minidoka, and Power Counties, ID...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    .... The Refuge is located 12 miles northeast of Rupert, ID, in the Snake River Plain, at approximately 4,200 feet in elevation. The area was historically comprised of a portion of the Snake River surrounded... impounded the Snake River and created Lake Walcott to store water for irrigation, and provide...

  15. Socioeconomic profiles of native American communities: Duckwater Shoshone Reservation

    SciTech Connect

    Hamby, M.

    1991-10-01

    This report presents socioeconomic aspects of Native Americans of the Duckwater Shoshone Reservation. A survey is included concerning their views on the proposed Yucca Mountain waste repository. (CBS)

  16. 76 FR 12753 - Notice of Temporary Closures on Public Lands in Ada and Elmore Counties, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ... hereby given that the Big Fire ( FNW4) and Hot Tea Fire ( FTF6) closures to motorized vehicle use are in... this area. The Hot Tea closure affects public lands in Elmore County, Idaho, burned August 27-29, 2010, by the Hot Tea Fire, 12 miles northwest of Mountain Home, Idaho. Unburned public lands in the...

  17. 76 FR 29784 - Notice of Realty Action; Direct Sale of Public Lands in Jerome County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-23

    ... owners of the adjacent private land, Todd and Bridget Buschhorn, for the appraised fair market value of... than the appraised fair market value: Boise Meridian T. 10 S., R. 19 E., sec. 25, lot 10. The area described contains 7.45 acres in Jerome County. The appraised fair market value is $5,600. The public...

  18. 75 FR 39580 - Notice of Realty Action; Direct Sale of Public Lands in Minidoka County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-09

    ...The Bureau of Land Management (BLM) proposes to sell four parcels of public land totaling 262.11 acres in Minidoka County, Idaho, to the owners of the surrounding private land for the appraised fair market value of $85,200. The private land surrounding the public land is owned by the Western Mortgage and Realty...

  19. Socioeconomic profiles of native American communities: Yomba Shoshone Reservation

    SciTech Connect

    Hamby, M.; Rusco, E.

    1991-10-01

    This report was written by the State of Nevada Agency for Nuclear Projects/Nuclear Waste Project Office. This office oversees the nuclear waste activities for the proposed Yucca Mountain high-level waste facility for the state of Nevada. The Yomba Shoshone Reservation socio-economic profile was the basis of this paper. It describes the life and current status of the Shoshone Indians. Population, utilities, education and social services of the Shoshone are examples of the topics which are discussed. It is intended as base-line information only. It eventually summarizes and compares data from the public opinion of the Shoshone about the high level waste repository at Yucca Mountain. (MB)

  20. 43 CFR 418.6 - Fallon Paiute-Shoshone Indian Reservation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false Fallon Paiute-Shoshone Indian Reservation... General Provisions § 418.6 Fallon Paiute-Shoshone Indian Reservation. Nothing in this part affects: (a) The authority of the Fallon Paiute-Shoshone Tribe to use water on the Tribe's reservation which...

  1. Shoshones in the Rocky Mountain Area. Student Workbook.

    ERIC Educational Resources Information Center

    Hultkrantz, Ake; And Others

    Derived from a monograph of the same title by Ake Hultkrantz, this student workbook contains seven articles which have been adapted for classroom use. Several student activities follow each article. Describing the Shoshones (who have historically lived in the neighborhood of the Rocky Mountains), the articles are titled: Historical Groupings,…

  2. 75 FR 70029 - Notice of Temporary Closure of Castle Rocks Inter-Agency Recreation Area in Cassia County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... Bureau of Land Management Notice of Temporary Closure of Castle Rocks Inter-Agency Recreation Area in...- Agency Recreation Area that are administered by the Burley Field Office, Bureau of Land Management. DATES... the BLM at Castle Rocks Inter-Agency Recreation Area in Cassia County, Idaho. Lands in the...

  3. 77 FR 52680 - Foreign-Trade Zone 242-Boundary County, ID, Notification of Proposed Production Activity, AREVA...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-30

    ... AREVA Enrichment Services, LLC (AES), located in Bonneville County, Idaho. The notification conforming.... A separate application for subzone status at the AES facility was submitted and will be processed... ] procedures could allow AES to choose the duty rates during customs entry procedures that apply to...

  4. OVERVIEW OF SPILLWAY FROM SOUTH BANK OF RIVER; FADING NORTHNORTHEAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OVERVIEW OF SPILLWAY FROM SOUTH BANK OF RIVER; FADING NORTH-NORTHEAST - Shoshone Falls Hydroelectric Project, Reservoir and Dam Complex, North Bank of Snake River, extreme Eastern end of the Shoshone Falls Hydroelectric Project, Tipperary Corner, Jerome County, ID

  5. OVERVIEW OF POWERHOUSES FROM SOUTH SIDE OF RIVER; FACING NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OVERVIEW OF POWERHOUSES FROM SOUTH SIDE OF RIVER; FACING NORTH - Shoshone Falls Hydroelectric Project, Reservoir and Dam Complex, North Bank of Snake River, extreme Eastern end of the Shoshone Falls Hydroelectric Project, Tipperary Corner, Jerome County, ID

  6. POWERHOUSES, SOUTH ELEVATIONS SHOWING OIL HOUSE, FILER SUBSTATION, AND DRAFT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    POWERHOUSES, SOUTH ELEVATIONS SHOWING OIL HOUSE, FILER SUBSTATION, AND DRAFT TUBE OUTLET (TAILRACE), VERTICAL VIEW; FACING WEST - Shoshone Falls Hydroelectric Project, Canyon Road, North Bank of Snake River below Shoshone Falls, Tipperary Corner, Jerome County, ID

  7. 43 CFR 418.6 - Fallon Paiute-Shoshone Indian Reservation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false Fallon Paiute-Shoshone Indian Reservation. 418.6 Section 418.6 Public Lands: Interior Regulations Relating to Public Lands BUREAU OF RECLAMATION... General Provisions § 418.6 Fallon Paiute-Shoshone Indian Reservation. Nothing in this part affects:...

  8. 43 CFR 418.6 - Fallon Paiute-Shoshone Indian Reservation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Fallon Paiute-Shoshone Indian Reservation. 418.6 Section 418.6 Public Lands: Interior Regulations Relating to Public Lands BUREAU OF RECLAMATION... General Provisions § 418.6 Fallon Paiute-Shoshone Indian Reservation. Nothing in this part affects:...

  9. Completion Report for Well ER-12-4, Corrective Action Unit 99: Rainier Mesa - Shoshone Mountain (includes Errata Sheet)

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Bechtel Nevada

    2006-05-01

    Well ER-12-4 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in May 2005, as part of a hydrogeologic investigation program for the Rainier Mesa-Shoshone Mountain Corrective Action Unit in the north-central portion of the Nevada Test Site. The well is located on Rainier/Aqueduct Mesa, northwest of Yucca Flat, within Area 12 of the Nevada Test Site. The well provided information regarding the radiological and physical environment near underground nuclear tests conducted in U12t Tunnel, information on the pre-Tertiary rocks in the area, and depth to the regional water table.

  10. Completion Report for Well ER-16-1 Corrective Action Unit 99: Rainier Mesa - Shoshone Mountain

    SciTech Connect

    NSTec Environmental Management

    2006-12-01

    Well ER-16-1 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. The well was drilled in June and July 2005 as part of a hydrogeologic investigation program for the Rainier Mesa-Shoshone Mountain Corrective Action Unit, Number 99. The overall purpose of the well was to gather subsurface data to better characterize the hydrogeology of the Shoshone Mountain area, especially in the older Tertiary and pre-Tertiary strata. The main 46.99-centimeter hole was drilled to a depth of 702.9 meters and cased with 33.97-centimeter casing to 663.7 meters. The hole diameter was then decreased to 31.1 centimeters, and the well was drilled to total depth of 1,220.7 meters. A completion string set at the depth of 1,162.4 meters consisted of 13.97-centimeter stainless-steel casing, with one continuous slotted interval open to the lower carbonate aquifer. The fluid level in the borehole soon dropped, so the borehole was deepened in July 2006. To deepen the borehole, the slotted section was cemented and a 12.1-centimeter hole was drilled through the bottom of the completion string to the new total depth of 1,391.7 meters, which is 171.0 meters deeper than the original borehole. A string of 6.03-centimeter carbon-steel tubing with one continuous slotted interval at 1,361.8 to 1,381.4 meters, and open to the lower carbonate aquifer, was installed in the well with no gravel packing or cement, to serve as a monitoring string. Data gathered during and shortly after hole construction include composite drill cuttings samples collected every 3 meters (extra cuttings samples were collected from the Paleozoic rocks for paleontological analyses), sidewall core samples from 37 depths, various geophysical logs, and water level measurements. These data indicate that the well penetrated 646.8 meters of Tertiary volcanic rocks and 744.9 meters

  11. 6. Historic American Buildings Survey William C. Everhart, Photographer October ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Historic American Buildings Survey William C. Everhart, Photographer October 1958 FRONT and WEST SIDE ELEVATIONS - Sacred Heart Mission, Interstate 90 & Interchange 39, Cataldo, Shoshone County, ID

  12. 3. Historic American Buildings Survey William C. Everhart, Photographer October ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey William C. Everhart, Photographer October 1958 FRONT and EAST SIDE ELEVATIONS - Sacred Heart Mission, Interstate 90 & Interchange 39, Cataldo, Shoshone County, ID

  13. 5. Historic American Buildings Survey Paul J. F. Schumacher, Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey Paul J. F. Schumacher, Photographer September 1957 FRONT and WEST SIDE ELEVATIONS - Sacred Heart Mission, Interstate 90 & Interchange 39, Cataldo, Shoshone County, ID

  14. 1921 POWERHOUSE, EAST AND NORTH ELEVATIONS, VERTICAL VIEW; FACING WESTSOUTHWEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1921 POWERHOUSE, EAST AND NORTH ELEVATIONS, VERTICAL VIEW; FACING WEST-SOUTHWEST - Shoshone Falls Hydroelectric Project, 1921 Powerhouse, North Bank of Snake River, Tipperary Corner, Jerome County, ID

  15. Completion Report for Well ER-12-3. Corrective Action Unit 99: Rainier Mesa - Shoshone Mountain

    SciTech Connect

    None, None

    2006-05-01

    Well ER-12-3 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. The well was drilled in March and April 2005 as part of a hydrogeologic investigation program for the Rainier Mesa-Shoshone Mountain Corrective Action Unit. The overall purpose of the well was to gather subsurface data to better characterize the hydrogeology of central Rainier Mesa, especially in the older Tertiary volcanic rocks and Paleozoic sedimentary rocks. The main 47.0-centimeter hole was drilled to a depth of 799.2 meters and cased with 33.97-centimeter casing to 743.1 meters. The hole diameter was then decreased to 31.1 centimeters, and the well was drilled to a total depth of 1,496.0 meters. The completion string consisted of 13.97-centimeter stainless steel casing, with two slotted intervals open to the lower carbonate aquifer, suspended from 19.37-centimeter carbon steel casing. A piezometer string was installed outside the 33.97-centimeter casing to a depth of 467.1 meters to monitor a zone of perched water within the Tertiary volcanic section. Data gathered during and shortly after hole construction include composite drill cuttings samples collected every 3 meters (extra cuttings samples were collected from the Paleozoic rocks for paleontological analyses), sidewall core samples from 35 depths, various geophysical logs, and water level measurements. These data indicate that the well penetrated 674.2 meters of Tertiary volcanic rocks and 821.7 meters of Paleozoic dolomite and limestone. Forty-nine days after the well was completed, but prior to well development and testing, the water level inside the main hole was tagged at the depth of 949.1 meters, and the water level inside the piezometer string was tagged at 379.9 meters.

  16. 78 FR 76829 - Approval of Application Submitted by Eastern Shoshone Tribe and Northern Arapaho Tribe for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ...This notice announces that the EPA Regional Administrator for Region 8 has approved the December 2008 application submitted by the Northern Arapaho Tribe and Eastern Shoshone Tribe (Tribes) of the Wind River Indian Reservation for treatment in a similar manner as a state (TAS) pursuant to the Clean Air Act and the EPA's implementing regulations for purposes of certain Clean Air Act provisions.......

  17. Hopis, Western Shoshones, and Southern Utes: Three Different Responses to the Indian Reorganization Act of 1934.

    ERIC Educational Resources Information Center

    Clemmer, Richard O.

    1986-01-01

    Compares the impact of the Indian Reorganization Act of 1934 on the Hopis, Western Shoshones, and Southern Utes with regard to land reform, economic development, and tribal management. Describes salient tribal characteristics prior to 1934, and federal government tactics to force acceptance of the Act. Contains 83 references. (SV)

  18. 76 FR 14058 - Notice of Inventory Completion: Fremont County Coroner, Riverton, WY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-15

    ... notice. A detailed assessment of the human remains was made by the Fremont County Coroner professional staff in consultation with representatives of the Shoshone Tribe of the Wind River Reservation, Wyoming... Wind River Reservation, but subsequently transferred and is no longer reservation land. The area of...

  19. PROCESS WATER BUILDING, TRA605. SUMP TANK PUMP. COMPARE WITH ID33G247. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PROCESS WATER BUILDING, TRA-605. SUMP TANK PUMP. COMPARE WITH ID-33-G-247. INL NEGATIVE NO. 4378. Unknown Photographer, 3/5/1952 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  20. Geophysical investigation of hot springs in the vicinity of Shoshone, California

    NASA Astrophysics Data System (ADS)

    Dziedzorm, Ziwu Felix

    Magnetic and gravity surveys were conducted in the vicinity of Shoshone, California to test a hypothesis from investigating hotsprings within Tecopa and Saratoga which states that deep faults must intersect with a specific orientation relative to the regional stress field in order to create hot springs along the Amargosa River. Three isolated basalt flows with different gravity and magnetic properties were identified near the Shoshone hot spring. Two of the flows have very low magnetic anomalies and the remaining one has a high magnetic anomaly. The high magnetic anomaly basalt flow also has a significantly higher Bouguer anomaly than the other two flows associated with the low magnetic anomalies. These observations suggest that the flows were formed by different time volcanic activity with the low magnetic anomaly basalts cooling during a magnetic reversal and the high magnetic anomaly basalt cooling during normal magnetic era. The deepest part of the Tecopa basin in the study area was identified as a region with low Bouguer anomaly and associated magnetic high. These properties suggest the basin fill includes highly vesicular basalts which would give the low density. Generally regions of high magnetic anomalies also have high Bouguer anomalies which indicate the possible existence of igneous rocks in the region. The low magnetic anomalies are mainly seen in the regions of thicker sedimentary deposits such as in the Resting Springs range where Precambrian and Paleozoic sedimentary rocks are preserved. From the magnetic and gravity surveys, this research support the testing hypothesis based on the identified intersecting faults of the appropriate orientation associated with the Shoshone hot spring. Faults were also identified at places with no evidence of hot springs and this could be due to the presence of thicker sediments preventing the springs from flowing to the surface. An alternative possibility is that the faults are not deep enough to tap the hot water

  1. Magnetotelluric Data, Rainier Mesa/Shoshone Mountain, Nevada Test Site, Nevada.

    SciTech Connect

    Jackie M. Williams; Jay A. Sampson; Brian D. Rodriguez; and Theodore H. Asch.

    2006-11-03

    The United States Department of Energy (DOE) and the National Nuclear Security Administration (NNSA) at their Nevada Site Office (NSO) are addressing ground-water contamination resulting from historical underground nuclear testing through the Environmental Management (EM) program and, in particular, the Underground Test Area (UGTA) project. From 1951 to 1992, 828 underground nuclear tests were conducted at the Nevada Test Site northwest of Las Vegas. Most of these tests were conducted hundreds of feet above the ground-water table; however, more than 200 of the tests were near or within the water table. This underground testing was limited to specific areas of the Nevada Test Site, including Pahute Mesa, Rainier Mesa/Shoshone Mountain, Frenchman Flat, and Yucca Flat. One issue of concern is the nature of the somewhat poorly constrained pre-Tertiary geology, and its effects on ground-water flow. Ground-water modelers would like to know more about the hydrostratigraphy and geologic structure to support a hydrostratigraphic framework model that is under development for the Rainier Mesa/Shoshone Mountain Corrective Action Unit (Bechtel Nevada, 2006). During 2005, the U.S. Geological Survey (USGS), in cooperation with the DOE and NNSA-NSO, collected and processed data from twenty-six magnetotelluric (MT) and audio-magnetotelluric (AMT) sites at the Nevada Test Site. The 2005 data stations were located on and near Rainier Mesa and Shoshone Mountain to assist in characterizing the pre-Tertiary geology in those areas. These new stations extend the area of the hydrogeologic study previously conducted in Yucca Flat. This work will help refine what is known about the character, thickness, and lateral extent of pre-Tertiary confining units. In particular, a major goal has been to define the upper clastic confining unit (UCCU – late Devonian to Mississippian-age siliciclastic rocks assigned to the Eleana Formation and Chainman Shale) from the Yucca Flat area and west towards

  2. ETR BASEMENT, TRA642, INTERIOR. BASEMENT. CUBICLE INTERIOR (SEE PHOTOS ID33G101 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ETR BASEMENT, TRA-642, INTERIOR. BASEMENT. CUBICLE INTERIOR (SEE PHOTOS ID-33-G-101 AND ID-33-G-102) WITH TANK AND SODIUM-RELATED APPARATUS. CAMERA STANDS BEFORE ROLL-UP DOOR SHOWN IN PHOTO ID-33-G-101. INL NEGATIVE NO. HD24-3-3. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  3. Deep Resistivity Structure of Rainier Mesa-Shoshone Mountain, Nevada Test Site, Nevada

    SciTech Connect

    Theodore H. Asch; Brian D. Rodriguez; Jay A. Sampson; Jackie M. Williams; Maryla Deszcz-Pan

    2006-12-12

    The U. S. Department of Energy (DOE) and the National Nuclear Security Administration (NNSA) at their Nevada Site Office (NSO) are addressing groundwater contamination resulting from historical underground nuclear testing through the Environmental Management (EM) program and, in particular, the Underground Test Area (UGTA) project. During 2005, the U.S. Geological Survey (USGS), funded by the DOE and NNSA-NSO, collected and processed data from twenty-six Magnetotelluric (MT) and Audio-Magnetotelluric (AMT) sites at the Nevada Test Site. Data stations were located in and near Rainier Mesa and Shoshone Mountain to assist in characterizing the pre-Tertiary geology in those areas. These new stations extend to the west the hydrogeologic study that was conducted in Yucca Flat in 2003. This work has helped to refine the character, thickness, and lateral extent of pre Tertiary confining units. In particular, a major goal has been to define the upper clastic confining unit (UCCU – late Devonian to Mississippian-age siliciclastic rocks assigned to the Eleana Formation and Chainman Shale(Bechtel Nevada, 2006)) in the Yucca Flat area and west towards Shoshone Mountain in the south, east of Buckboard Mesa, and onto Rainier Mesa in the north. The Nevada Test Site magnetotelluric data interpretation presented in this report includes the results of detailed two-dimensional (2 D) resistivity modeling for each profile (including alternative interpretations) and gross inferences on the three dimensional (3 D) character of the geology within the region. The character, thickness, and lateral extent of the Chainman Shale and Eleana Formation that comprise the Upper Clastic Confining Unit (UCCU) are generally characterized in the upper 5 km. The interpretation is not well determined where conductive TCU overlies conductive Chainman Shale, where resistive Eleana Formation overlies resistive LCA units, or where resistive VTA rock overlies units of the Eleana Formation. The nature of the

  4. 2. Left side of Zinc Plant, from packless Cooling Tower ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Left side of Zinc Plant, from packless Cooling Tower to midpoint of Cell Room, with majority of Upper Plant in view. View is to the east. - Sullivan Electrolytic Zinc Plant, Government Gulch, Kellogg, Shoshone County, ID

  5. 4. Anode Building. View is to the east. This facility, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Anode Building. View is to the east. This facility, which was used for the cleaning and manufacture of anodes, was documented prior to demolition in 1993. - Sullivan Electrolytic Zinc Plant, Government Gulch, Kellogg, Shoshone County, ID

  6. 3. Right side of Zinc Plant, from Cell Room midpoint ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Right side of Zinc Plant, from Cell Room midpoint to Plant Office (foreground) and #5 Roaster and Concentrate Handling (background). View is to the east. - Sullivan Electrolytic Zinc Plant, Government Gulch, Kellogg, Shoshone County, ID

  7. 8. Historic American Buildings Survey John N. DeHaas, Jr., Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Historic American Buildings Survey John N. DeHaas, Jr., Photographer August 1963 EAST ELEVATION (Looking Northward) - Sacred Heart Mission, Interstate 90 & Interchange 39, Cataldo, Shoshone County, ID

  8. 10. Historic American Buildings Survey John N. DeHaas, Jr., Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Historic American Buildings Survey John N. DeHaas, Jr., Photographer August 1963 VIEW OF HAND CARVED CEILING PANEL - Sacred Heart Mission, Interstate 90 & Interchange 39, Cataldo, Shoshone County, ID

  9. 9. Historic American Buildings Survey John N. DeHaas, Jr., Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Historic American Buildings Survey John N. DeHaas, Jr., Photographer August 1963 VIEW OF HAND CARVED CEILING PANEL - Sacred Heart Mission, Interstate 90 & Interchange 39, Cataldo, Shoshone County, ID

  10. 2. Historic American Buildings Survey William C. Everhart, Photographer October ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey William C. Everhart, Photographer October 1958 VIEW FROM THE NORTHEAST (Residence of Caretaker at Left) - Sacred Heart Mission, Interstate 90 & Interchange 39, Cataldo, Shoshone County, ID

  11. 13. Historic American Buildings Survey John N. DeHaas, Jr., Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Historic American Buildings Survey John N. DeHaas, Jr., Photographer August 1963 COLUMN DETAILS AT APSE AND SIDE ALTAR - Sacred Heart Mission, Interstate 90 & Interchange 39, Cataldo, Shoshone County, ID

  12. AFRD WAREHOUSE, SOUTH SIDE. FACING NORTH. METAL AWNING ALONG LENGTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AFRD WAREHOUSE, SOUTH SIDE. FACING NORTH. METAL AWNING ALONG LENGTH OF BUILDING AND VERTICAL METAL SIDING ARE ALTERATIONS MAD BY THE AFRD. - Minidoka Relocation Center Warehouse, 111 South Fir Street, Shoshone, Lincoln County, ID

  13. TAILRACE, WITH POWERHOUSES (SOUTH ELEVATIONS), VERTICAL VIEW; FACING NORTHEAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    TAILRACE, WITH POWERHOUSES (SOUTH ELEVATIONS), VERTICAL VIEW; FACING NORTHEAST - Shoshone Falls Hydroelectric Project, Tailrace, North Bank of Snake River, South side of powerhouse complex, Tipperary Corner, Jerome County, ID

  14. SKIDWAY AND WEST ELEVATION OF 1921 POWERHOUSE, GROUND FLOOR ENTRY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SKIDWAY AND WEST ELEVATION OF 1921 POWERHOUSE, GROUND FLOOR ENTRY AT LOWER RIGHT, VERTICAL VIEW; FACING NORTHEAST - Shoshone Falls Hydroelectric Project, Skidway, North Bank of Snake River, immediately North/Northeast of the 1921 Powerhouse, Tipperary Corner, Jerome County, ID

  15. AFRD WAREHOUSE, SOUTH SIDE DETAIL, SLIDING DOORS. FACING NORTH. COMPARE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AFRD WAREHOUSE, SOUTH SIDE DETAIL, SLIDING DOORS. FACING NORTH. COMPARE WITH FIGURES 4 AND 5 IN NARRATIVE REPORT, WHICH SHOW DIAGONAL BRACES BELOW GLAZED SECTION OF DOORS. - Minidoka Relocation Center Warehouse, 111 South Fir Street, Shoshone, Lincoln County, ID

  16. 1. Zinc Plant, looking north, down Government Gulch. 610 ft. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Zinc Plant, looking north, down Government Gulch. 610 ft. tall stack replaced original 200 ft. radial brick stack formerly at rear of Cottrell treater. - Sullivan Electrolytic Zinc Plant, Government Gulch, Kellogg, Shoshone County, ID

  17. Tribal Wind Assessment by the Eastern Shoshone Tribe of the Wind River Reservation

    SciTech Connect

    Pete, Belvin; Perry, Jeremy W.; Stump, Raphaella Q.

    2009-08-28

    The Tribes, through its consultant and advisor, Distributed Generation Systems (Disgen) -Native American Program and Resources Division, of Lakewood CO, assessed and qualified, from a resource and economic perspective, a wind energy generation facility on tribal lands. The goal of this feasibility project is to provide wind monitoring and to engage in preproject planning activities designed to provide a preliminary evaluation of the technical, economic, social and environmental feasibility of developing a sustainable, integrated wind energy plan for the Eastern Shoshone and the Northern Arapahoe Tribes, who resides on the Wind River Indian Reservation. The specific deliverables of the feasibility study are: 1) Assessments of the wind resources on the Wind River Indian Reservation 2) Assessments of the potential environmental impacts of renewable development 3) Assessments of the transmission capacity and capability of a renewable energy project 4) Established an economic models for tribal considerations 5) Define economic, cultural and societal impacts on the Tribe

  18. The Ids have it.

    PubMed

    Edwards, Lincoln A; Fine, Howard A

    2010-12-14

    In this issue of Cancer Cell, Anido et al. demonstrate that Id1 is the likely arbiter of divergent transforming growth factor-β (TGF-β) signaling in glioma-initiating cells (GICs) from different tumors. These findings hold both the promise and potential peril of therapeutic targeting of the TGF-β pathway.

  19. Not Another ID Model.

    ERIC Educational Resources Information Center

    Roberts, Stephanie; Conn, Cynthia; Lohr, Linda; Hunt, Erin; Duffy, Annmari

    2003-01-01

    Presents a meta-model of instructional design (ID) that integrates content covered in instructional technology graduate programs and explores the impact of an instructional designer's perspective on designs. Discusses the theoretical background; instructional strategies; educational philosophies; paradigm shifts; behaviorist, constructivist, and…

  20. OrbId

    PubMed Central

    Filshtein, Teresa J.; Mackenzie, Craig O.; Dale, Maurice D.; Dela-Cruz, Paul S.; Ernst, Dale M.; Frankenberger, Edward A.; He, Chunyan; Heath, Kaylee L.; Jones, Andria S.; Jones, Daniel K.; King, Edward R.; Maher, Maggie B.; Mitchell, Travis J.; Morgan, Rachel R.; Sirobhushanam, Sirisha; Halkyard, Scott D.; Tiwari, Kiran B.; Rubin, David A.; Borchert, Glen M.; Larson, Erik D.

    2012-01-01

    MicroRNAs coordinate networks of mRNAs, but predicting specific sites of interactions is complicated by the very few bases of complementarity needed for regulation. Although efforts to characterize the specific requirements for microRNA (miR) regulation have made some advances, no general model of target recognition has been widely accepted. In this work, we describe an entirely novel approach to miR target identification. The genomic events responsible for the creation of individual miR loci have now been described with many miRs now known to have been initially formed from transposable element (TE) sequences. In light of this, we propose that limiting miR target searches to transcripts containing a miR’s progenitor TE can facilitate accurate target identification. In this report we outline the methodology behind OrbId (Origin-based identification of microRNA targets). In stark contrast to the principal miR target algorithms (which rely heavily on target site conservation across species and are therefore most effective at predicting targets for older miRs), we find OrbId is particularly efficacious at predicting the mRNA targets of miRs formed more recently in evolutionary time. After defining the TE origins of > 200 human miRs, OrbId successfully generated likely target sets for 191 predominately primate-specific human miR loci. While only a handful of the loci examined were well enough conserved to have been previously evaluated by existing algorithms, we find ~80% of the targets for the oldest miR (miR-28) in our analysis contained within the principal Diana and TargetScan prediction sets. More importantly, four of the 15 OrbId miR-28 putative targets have been previously verified experimentally. In light of OrbId proving best-suited for predicting targets for more recently formed miRs, we suggest OrbId makes a logical complement to existing, conservation based, miR target algorithms. PMID:23087843

  1. Magnetotelluric Data, Rainier Mesa/Shoshone Mountain, Nevada Test Site, Nevada

    USGS Publications Warehouse

    Williams, Jackie M.; Sampson, Jay A.; Rodriguez, Brian D.; Asch, Theodore H.

    2006-01-01

    Introduction: The United States Department of Energy (DOE) and the National Nuclear Security Administration (NNSA) at their Nevada Site Office (NSO) are addressing ground-water contamination resulting from historical underground nuclear testing through the Environmental Management (EM) program and, in particular, the Underground Test Area (UGTA) project. During 2005, the U.S. Geological Survey (USGS), in cooperation with the DOE and NNSA-NSO, collected and processed data from twenty-six magnetotelluric (MT) and audio-magnetotelluric (AMT) sites at the Nevada Test Site. The 2005 data stations were located on and near Rainier Mesa and Shoshone Mountain to assist in characterizing the pre-Tertiary geology in those areas. These new stations extend the area of the hydrogeologic study previously conducted in Yucca Flat. The MT data presented in this report will help refine what is known about the character, thickness, and lateral extent of pre Tertiary confining units. Subsequent interpretation will include a three dimensional (3 D) character analysis and a two-dimensional (2 D) resistivity model. The purpose of this report is to release the MT sounding data. No interpretation of the data is included here.

  2. 39. CALCINER CELL PLANS. TOGETHER WITH HAER ID33C37 ILLUSTRATES COMPLEXITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. CALCINER CELL PLANS. TOGETHER WITH HAER ID-33-C-37 ILLUSTRATES COMPLEXITY OF PIPING. INEEL DRAWING NUMBER 200-0633-00-287-106445. FLUOR NUMBER 5775-CPP-633-P-50 - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  3. 40. CALCINER CELL SECTIONS. TOGETHER WITH HAER ID33C37 ILLUSTRATES COMPLEXITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    40. CALCINER CELL SECTIONS. TOGETHER WITH HAER ID-33-C-37 ILLUSTRATES COMPLEXITY OF PIPING. INEEL DRAWING NUMBER 200-0633-00-287-106446. FLUOR NUMBER 5775-CPP-P-51. - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  4. 13. TIP TOP MINE. WOODEN STRUCTURE VISIBLE IN ID31C12 ISIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. TIP TOP MINE. WOODEN STRUCTURE VISIBLE IN ID-31-C-12 ISIN UPPER LEFT. ALSO VISIBLE ARE TWO VERTICALLY BURIED LOGS ASSOCIATED WITH CABLES, ONE OF WHICH IS VISIBLE IN LOWER RIGHT. CAMERA POINTED SOUTH. - Florida Mountain Mining Sites, Tip Top Mine, West face Florida Mountain, approximately 150 feet below summit, Silver City, Owyhee County, ID

  5. Corrective Action Investigation Plan for Corrective Action Unit 99: Rainier Mesa/Shoshone Mountain, Nevada Test Site, Nevada with Errata and ROTC 1, Rev. No. 0

    SciTech Connect

    McCord, John; Marutzky, Sam

    2004-12-01

    This Corrective Action Investigation Plan (CAIP) was developed for Corrective Action Unit (CAU) 99, Rainier Mesa/Shoshone Mountain. The CAIP is a requirement of the ''Federal Facility Agreement and Consent Order'' (FFACO) agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense (DoD) (FFACO, 1996). The FFACO addresses environmental restoration activities at U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) facilities and sites including the underground testing area(s) of the Nevada Test Site (NTS). This CAIP describes the investigation activities currently planned for the Rainier Mesa/Shoshone Mountain CAU. These activities are consistent with the current Underground Test Area (UGTA) Project strategy described in Section 3.0 of Appendix VI, Revision No. 1 (December 7, 2000) of the FFACO (1996) and summarized in Section 2.1.2 of this plan. The Rainier Mesa/Shoshone Mountain CAU extends over several areas of the NTS (Figure 1-1) and includes former underground nuclear testing locations in Areas 12 and 16. The area referred to as ''Rainier Mesa'' includes the geographical area of Rainier Mesa proper and the contiguous Aqueduct Mesa. Figure 1-2 shows the locations of the tests (within tunnel complexes) conducted at Rainier Mesa. Shoshone Mountain is located approximately 20 kilometers (km) south of Rainier Mesa, but is included within the same CAU due to similarities in their geologic setting and in the nature and types of nuclear tests conducted. Figure 1-3 shows the locations of the tests conducted at Shoshone Mountain. The Rainier Mesa/Shoshone Mountain CAU falls within the larger-scale Rainier Mesa/Shoshone Mountain Investigation Area, which also includes the northwest section of the Yucca Flat CAU as shown in Figure 1-1. Rainier Mesa and Shoshone Mountain lie adjacent to the Timber Mountain Caldera Complex and are composed of volcanic rocks that erupted from the

  6. Coupled variations in helium isotopes and fluid chemistry: Shoshone Geyser Basin, Yellowstone National Park

    SciTech Connect

    Hearn, E.H.; Kennedy, B.M. ); Truesdell, A.H. )

    1990-11-01

    Early studies of {sup 3}He/{sup 4}He variations in geothermal systems have generally attributed these fluctuations to either differences in the source of the magmatic {sup 3}He-rich helium or to local differences in the deep flux of magmatic {sup 3}He-rich helium. Kennedy et al, however, show that near-surface processes such as boiling and dilution may also drastically affect {sup 3}He/{sup 4}He ratios of geothermal vapors. Helium isotope ratios were determined for several hot springs at Shoshone Geyser Basin of Yellowstone National Park for this study, along with other noble gas data. Stable isotope data and water and gas chemistry data for each spring were also compiled. The water chemistry indicates that there is one deep, hot thermal water in the area which is mixing with dilute meteoric water that has entered the system at depth. Spring HCO{sub 3}{sup {minus}} concentrations correlate with {sup 3}He/{sup 4}He values, as in nearby Lower Geyser Basin. This correlation is attributed to variable amounts of deep dilution of thermal waters with a relatively cool water that inhibits boiling at depth, thus preventing the loss of CO{sub 2} and magmatic He in the most diluted samples. Oxygen and hydrogen isotope data also support a boiling and dilution model, but to produce the observed fractionations, the boiling event would have to be extensive, with steam loss at the surface, whereas the boiling that affected the helium isotope ratios was probably a small scale event with steam loss at depth. It is possible that deep boiling occurred in the basin and that small amounts of steam escaped along fractures at about 500 m below the surface while all subsequently produced steam was lost near or at the surface.

  7. Coupled variations in helium isotopes and fluid chemistry: Shoshone Geyser Basin, Yellowstone National Park

    USGS Publications Warehouse

    Hearn, E.H.; Kennedy, B.M.; Truesdell, A.H.

    1990-01-01

    Early studies of 3He/4He variations in geothermal systems have generally attributed these fluctuations to either differences in the source of the magmatic 3He-rich helium or to local differences in the deep flux of magmatic 3He-rich helium. Kennedy et al. (1987), however, show that near-surface processes such as boiling and dilution may also drastically affect 3He 4He ratios of geothermal vapors. Helium isotope ratios were determined for several hot springs at Shoshone Geyser Basin of Yellowstone National Park for this study, along with other noble gas data. Stable isotope data and water and gas chemistry data for each spring were also compiled. The water chemistry indicates that there is one deep, hot thermal water in the area which is mixing with dilute meteoric water that has entered the system at depth. Spring HCO3- concentrations correlate with 3He 4He values, as in nearby Lower Geyser Basin. This correlation is attributed to variable amounts of deep dilution of thermal waters with a relatively cool water that inhibits boiling at depth, thus preventing the loss of CO2 (and therefore HCO3-) and magmatic He in the most diluted samples. Oxygen and hydrogen isotope data also support a boiling and dilution model, but to produce the observed fractionations, the boiling event would have to be extensive, with steam loss at the surface, whereas the boiling that affected the helium isotope ratios was probably a small scale event with steam loss at depth. It is possible that deep boiling occurred in the basin and that small amounts of steam escaped along fractures at about 500 m below the surface while all subsequently produced steam was lost near or at the surface. ?? 1990.

  8. DHSIsotopeID

    2007-12-18

    DHSIsotopeID is an application designed to read and analyze radiation measurement files taken by radiation measurement files taken by radiation portal monitors, and in particular, by the advanced spectroscopic portals. It requires that the data files be in the N42 file format, compliant with the interface control documents for DNDO radiation measurement files. It carries out an automated analysis to determine which isotopes are present in the spectra, and then presents the results in graphicalmore » form to the user. It also enables further post-processing and analysis, for example by performing further analysis on selected regions of interest of the spectrum, as designated by the user via the graphical interface.« less

  9. IDS contribution to ITRF2008

    NASA Astrophysics Data System (ADS)

    Valette, J.; Lemoine, F.; Ferrage, P.; Altamimi, Z.; Willis, P.; Stepanek, P.; Otten, M.; Govind, R.; Kuzin, S.; Le Bail, K.; Moore, P.; Yaya, P.; Soudarin, L.

    2009-12-01

    The International DORIS Service (IDS), in operation since 2003, submitted three sets of solutions to ITRF2005 from the IGN/JPL, LEGOS/CLS, and INASAN analysis centers, but no DORIS technique combination. Since that time new analysis centers have become operational including the Geodetic Observatory Pecny (GOP), the European Space Operations Center (ESOC), Geoscience Australia (GAU), the NASA Goddard Space Flight Center (GSC), and the University of Newcastle (NCL). These analysis centers run different software, including Gypsy (IGN & INASAN), GINS (LCA), Bernese (GOP), NAPEOS (ESOC), GEODYN (Geoscience Australia and NASA GSFC) and FAUST (NCL). In order to contribute to ITRF2008, seven analysis centers processed DORIS data from TOPEX/Poseidon, SPOT2, SPOT3, SPOT4, SPOT5, and ENVISAT from 1992 to 2008, producing weekly SINEX solutions or normal equations. The weekly SINEX files from seven AC’s were processed with the CATREF software. Three iterations of an IDS weekly combined time series were completed. The IDS-1, and IDS-2 combinations were preliminary station-only solutions. In the final combination, IDS-3, both stations and the Earth Orientation Paramters (EOP’s) were adjusted. Between each of the IDS combinations, the combination strategy (station filtering, outliers, weighting, scale or geocenter contributions) was improved and the AC’s SINEX series were refined. Some series were extended in data span while others were recomputed to correct anomalies or to improve the quality of the submissions, based on feedback from the combination analyses and intercenter comparisons. For example in IDS-1, both the GAU and GSC solutions were affected by a 20 mm scale offset that was removed in IDS-2 and IDS-3 after the application of improved troposphere modelling in the GEODYN software. The analysis for IDS-1 showed a higher station position WRMS in the vicinity of the high solar flux periods (late 2001-2002). Consequently for IDS-2, several AC’s (LCA, GAU, GSC

  10. SNP ID-info: SNP ID searching and visualization platform.

    PubMed

    Yang, Cheng-Hong; Chuang, Li-Yeh; Cheng, Yu-Huei; Wen, Cheng-Hao; Chang, Phei-Lang; Chang, Hsueh-Wei

    2008-09-01

    Many association studies provide the relationship between single nucleotide polymorphisms (SNPs), diseases and cancers, without giving a SNP ID, however. Here, we developed the SNP ID-info freeware to provide the SNP IDs within inputting genetic and physical information of genomes. The program provides an "SNP-ePCR" function to generate the full-sequence using primers and template inputs. In "SNPosition," sequence from SNP-ePCR or direct input is fed to match the SNP IDs from SNP fasta-sequence. In "SNP search" and "SNP fasta" function, information of SNPs within the cytogenetic band, contig position, and keyword input are acceptable. Finally, the SNP ID neighboring environment for inputs is completely visualized in the order of contig position and marked with SNP and flanking hits. The SNP identification problems inherent in NCBI SNP BLAST are also avoided. In conclusion, the SNP ID-info provides a visualized SNP ID environment for multiple inputs and assists systematic SNP association studies. The server and user manual are available at http://bio.kuas.edu.tw/snpid-info.

  11. Low-angle slumps in lacustrine environments: the Shoshone (Tecopa basin, California) example

    NASA Astrophysics Data System (ADS)

    Alfaro, Pedro; Gibert, Luis; García Tortosa, Francisco J.; Owen, Geraint; Moretti, Massimo; Scott, Gary

    2015-04-01

    In lacustrine environments, the presence of seismically-induced slump sheets on gentle slopes seems to be very widespread (Alsop and Marco, 2013; García-Tortosa et al., 2011; Gibert et al., 2005). A low-angle slump is extraordinarily well exposed in middle Pleistocene lacustrine deposits of the Tecopa Basin (California, United States), next to the village of Shoshone. Good lateral continuity of stratigraphical sections allowed a detailed description of the slump sheet and a reliable interpretation taking into account main morphological features, data coming from the facies analysis of the involved beds and on the regional tectonic activity. The slump sheet, ~ 2 m thick, is constituted by four units with different rheological characteristics. This heterogeneous composition is the key controlling factor which produces a large variety of soft-sediment deformational structures of different scale (folds, thrusts, breccias, fluidization structures). An earthquake related to regional faults might cause liquefaction and fluidization, and the initiation of the gravitational movement. When slump translation ceases, at the toe of the slump, then contractional strain propagates upslope and internal deformation occurs in the slump sheet. The top bed of the slump sheet, ~1 m of sands, developed decametric-scale folds while underlying sediments migrates laterally from the synform hinges to the core of the anticlines. Different velocity gradients in the downslope direction generate several superposed layer-parallel shear with two main detachments and many secondary internal ones. This new example in the Tecopa Basin, confirms that slumps are a good record of seismicity in low-angle sedimentary environments. Alsop, G.I. & Marco, S. (2013): Seismogenic slump folds formed by gravity-driven tectonics down a negligible subaqueous slope. Tectonophysics, 605: 48-69. García-Tortosa, F.J., Alfaro, P., Gibert, L. & Scott, G (2011): Seismically induced slump on an extremely gentle slope

  12. ETR BUILDING, TRA642, INTERIOR. BASEMENT. CUBICLE SHOWN IN ID33G101, ANOTHER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    ETR BUILDING, TRA-642, INTERIOR. BASEMENT. CUBICLE SHOWN IN ID-33-G-101, ANOTHER VIEW. PERSONNEL DOORWAY INTO CHAMBER IDENTIFIES SODIUM HAZARD AND POSSIBILITY OF INERT GAS. LIQUID SODIUM COOLANT WAS USED IN A SPECIAL ETR LOOP ADAPTED FOR IT IN 1972. INL NEGATIVE NO. HD24-3-2. Mike Crane, Photographer, 11/2000 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  13. Risk concerns, land use, stewardship, and the Idaho National Engineering and Environmental Laboratory: attitudes of the Shoshone-Bannock and other American Indians.

    PubMed

    Burger, J; Roush, D E; Ramos, R; Gochfeld, M

    2000-07-01

    This paper examines the attitudes and perceptions of 277 American Indians about hunting and fishing, risk, and future land use of the Idaho National Engineering and Environmental Laboratory (INEEL) in southeastern Idaho. Nearly half of our sample were Shoshone-Bannock tribal members living on the nearby Fort Hall Reservation, and half were American Indians from elsewhere in the western United States. We also interviewed an additional 44 White people. We examine the hypothesis that there are differences in environmental concerns and attitudes toward future land use at IN-EEL as a function of tribal affiliation (ethnicity), educational level, gender, and age. Such perceptions are important because of the existence of tribal treaties that govern the legal and cultural rights of the Shoshone-Bannock. Returning INEEL to the Shoshone-Bannock Tribes, hunting, fishing, camping, hiking, and a National Environmental Research Park ranked as the highest preferred future land uses, whereas continuing nuclear materials reprocessing and increasing the storage of nuclear wastes ranked as the lowest. There were tribal differences in land use preferences, with those of the Fort Hall Indians being more similar to those of the local Whites than to other American Indians. All groups ranked storage of nuclear material, storage of additional nuclear material, and spills and accidents as the most serious of a list of concerns provided about the site. Fort Hall Indians answered an open-ended question with concerns for population levels and migration routes of game animals and other wildlife, more than hunting and human health. The Shoshone-Bannock from Fort Hall showed an environmental sensitivity for the well-being of wildlife and the health of the ecosystem and were interested in long-term stewardship, in addition to 29 concern for human health.

  14. 3. BUNKER HILL LEAD SMELTER. VIEW IS FROM CIA TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. BUNKER HILL LEAD SMELTER. VIEW IS FROM CIA TO THE SOUTHWEST. BUILDINGS NOTED IN ID-29-2 APPEAR, IN ADDITION TO DRY ORE PLANT AND BONNOT COAL PULVERIZING EQUIPMENT BUILDING ON THE RIGHT. - Bunker Hill Lead Smelter, Bradley Rail Siding, Kellogg, Shoshone County, ID

  15. ISS/IDS Detector Study

    SciTech Connect

    Cervera-Villanueva, A.

    2008-02-21

    This article summarises the results obtained by the detector working group of the 'International Scooping Study' (ISS) of a future neutrino oscillations facility. Special emphasis is put on far detectors, for which some of the main issues are identified. A detector R and D strategy in the context of the 'International Design Study' (IDS) for a neutrino factory is also presented.

  16. Threshold extended ID3 algorithm

    NASA Astrophysics Data System (ADS)

    Kumar, A. B. Rajesh; Ramesh, C. Phani; Madhusudhan, E.; Padmavathamma, M.

    2012-04-01

    Information exchange over insecure networks needs to provide authentication and confidentiality to the database in significant problem in datamining. In this paper we propose a novel authenticated multiparty ID3 Algorithm used to construct multiparty secret sharing decision tree for implementation in medical transactions.

  17. Raster Images of Geologic Maps of Middle Proterozoic Belt strata in parts of Benewah, Bonner, Kootenai and Shoshone Counties, Idaho and Lincoln, Mineral and Sanders Counties, Montana

    USGS Publications Warehouse

    Boleneus, David E.; Appelgate, Larry M.; Joseph, Nancy L.; Brandt, Theodore R.

    2001-01-01

    Geologic maps of the western part of the Belt Basin of western Montana and northern Idaho were converted into digital raster (TIFF image) format to facilitate their manipulation in geographic information systems. The 85-mile x 100-mile map area mostly contains rocks belonging to the lower and middle Belt Supergroup. The area is of interest as these Middle Proterozoic strata contain vein-type lead-zinc-silver deposits in the Coeur d?Alene Mining District in the St. Regis and Revett formations and strata-bound copper-silver deposits, such as the Troy mine, within the Revett Formation. The Prichard Formation is also prospective for strata-bound lead-zinc deposits because equivalent Belt strata in southern British Columbia, Canada host the Sullivan lead-zinc deposit. Map data converted to digital images include 13 geological maps at scales ranging from 1:48,000 to 1:12,000. Geologic map images produced from these maps by color scanning were registered to grid tick coverages in a Universal Transverse Mercator (North American Datum of 1927, zone 11) projection using ArcView Image Analysis. Geo-registering errors vary from 10 ft to 114 ft.

  18. IDS contribution to ITRF2008

    NASA Astrophysics Data System (ADS)

    Valette, Jean-Jacques; Lemoine, Frank G.; Ferrage, Pascale; Yaya, Philippe; Altamimi, Zuheir; Willis, Pascal; Soudarin, Laurent

    2010-12-01

    For the first time, the International DORIS Service (IDS) has produced a technique level combination based on the contributions of seven analysis centers (ACs), including the European Space Operations Center (ESOC), Geodetic Observatory Pecny (GOP), Geoscience Australia (GAU), the NASA Goddard Space Flight Center (GSFC), the Institut Géographique National (IGN), the Institute of Astronomy, Russian Academy of Sciences (INASAN, named as INA), and CNES/CLS (named as LCA). The ACs used five different software packages to process the DORIS data from 1992 to 2008, including NAPEOS (ESA), Bernese (GOP), GEODYN (GAU, GSC), GIPSY/OASIS (INA), and GINS (LCA). The data from seven DORIS satellites, TOPEX/Poseidon, SPOT-2, SPOT-3, SPOT-4, SPOT-5, Envisat and Jason-1 were processed and all the analysis centers produced weekly SINEX files in either variance-covariance or normal equation format. The processing by the analysis centers used the latest GRACE-derived gravity models, forward modelling of atmospheric gravity, updates to the radiation pressure modelling to improve the DORIS geocenter solutions, denser parameterization of empirically determined drag coefficients to improve station and EOP solutions, especially near the solar maximum in 2001-2002, updated troposphere mapping functions, and an ITRF2005-derived station set for orbit determination, DPOD2005. The CATREF software was used to process the weekly AC solutions, and produce three iterations of an IDS global weekly combination. Between the development of the initial solution IDS-1, and the final solution, IDS-3, the ACs improved their analysis strategies and submitted updated solutions to eliminate troposphere-derived biases in the solution scale, to reduce drag-related degradations in station positioning, and to refine the estimation strategy to improve the combination geocenter solution. An analysis of the frequency content of the individual AC geocenter and scale solutions was used as the basis to define the

  19. One ID Card for the Entire Campus.

    ERIC Educational Resources Information Center

    Ridenour, David P.; Ferguson, Linda M.

    1986-01-01

    The implementation by Indiana State University of a machine-readable photo ID system for their food services prompted an investigation into the available alternatives and requirements for a more efficient all-University ID card system. The new ID system is described. (AUTHOR/MLW)

  20. A late Frasnian (Late Devonian) radiolarian, sponge spicule, and conodont fauna from the Slaven Chert, northern Shoshone Range, Roberts Mountains allochthon, Nevada

    USGS Publications Warehouse

    Boundy-Sanders, S. Q.; Sandberg, C.A.; Murchey, B.L.; Harris, A.G.

    1999-01-01

    Co-occuring conodonts, radiolarians, and sponge spicules from the type locality of the Slaven Chert, northern Shoshone Range, Nevada, indicate that the radiolarian and sponge spicule assemblage described herein correlates with the Late rhenana conodont Zone (late Frasnian). The moderately well preserved radiolarians are the first Frasnian-age fauna described from the Western Hemisphere. They include spumellarians, Ceratoikiscum, and Paleoscenidium, and a radiolarian which we have assigned to a new genus, Durahelenifore Boundy-Sanders and Murchey, with its type species, Durahelenifore robustum Boundy-Sanders and Murchey. Sponge spicules include umbellate microscleres of the Subclass Amphidiscophora, Order Hemidiscosa, previously documented only in Pennsylvanian and younger rocks.

  1. 113. ROCK CREEK SIPHON LOW LINE CANAL, TWIN FALLS COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    113. ROCK CREEK SIPHON LOW LINE CANAL, TWIN FALLS COUNTY, SOUTH OF KIMBERLY IDAHO; CLOSE-UP OF INLET SIDE OF SIPHON, NORTHWEST VIEW. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  2. 126. COTTONWOOD CREEK SIPHON, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    126. COTTONWOOD CREEK SIPHON, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, IDAHO; CLOSE-UP OF OUTLET SIDE OF SIPHON, SOUTH VIEW. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  3. 111. ROCK CREEK SIPHON LOW LINE CANAL, TWIN FALLS COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    111. ROCK CREEK SIPHON LOW LINE CANAL, TWIN FALLS COUNTY, SOUTH OF KIMBERLY IDAHO; OVERALL VIEW OF SIPHON, EAST VIEW. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  4. 129. COTTONWOOD CREEK SIPHON, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    129. COTTONWOOD CREEK SIPHON, TWIN FALLS COUNTY, SOUTH OF KIMBERLY, IDAHO; OUTLET SIDE OF SIPHON UNDER CANAL. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  5. 115. ROCK CREEK SIPHON LOW LINE CANAL, TWIN FALLS COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    115. ROCK CREEK SIPHON LOW LINE CANAL, TWIN FALLS COUNTY, SOUTH OF KIMBERLY IDAHO; WEST VIEW OF SIPHON CROSSING ROCK CREEK. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  6. IDS-C and IDS-sr: psychometric properties in depressed in-patients.

    PubMed

    Corruble, E; Legrand, J M; Duret, C; Charles, G; Guelfi, J D

    1999-12-01

    Sixty-eight depressed in-patients were assessed at admission (DO), and after 5 days (D5), ten days (D10) and 28 days (D28) of antidepressant treatment, with the Inventory for Depressive Symptomatology-Clinician (IDS-C) and the Inventory for Depressive Symptomatology-Self-Rated (IDS-SR) (Rush et al., 1986), the Montgomery and Asberg Depression Rating Scale (MADRS) (Montgomery and Asberg, 1979) and the depression factor of the Symptom Check List (SCL-90R) (Derogatis, 1977), in order to assess IDS-C and IDS-SR psychometric properties in depressed in-patients and to compare IDS-C to MADRS and IDS-SR to the SCL-90R depression factor. Most of the IDS-C and IDS-SR items were significantly correlated to the final score and the Cronbach alpha coefficients were high (0.75 for the IDS-C and 0.79 for the IDS-SR). Principal Component Analyses (PCA) showed three factors for both IDS-C and IDS-SR: 'depression', 'anxiety/arousal' and 'sleep/appetite'. These results suggest satisfactory internal consistency of IDS-C and IDS-SR. Concurrent validity of the IDS-C with the MADRS was high (r = 0.81), as well as concurrent validity of the IDS-SR with the SCL-90R depression factor (r = 0.84). Concerning sensitivity to change, the four scales were able to discriminate between different levels of severity of depression. Moreover, considering paired t-tests on score changes, IDS-C sensitivity to change may be higher than MADRS sensitivity to change, this phenomenon being related to the number of items and degrees but not to the item contents. Contrary to IDS-C and MADRS, IDS-SR and SCL-90R depression factor were not different in terms of sensitivity to change. Finally, psychometric properties of IDS-C and IDS-SR in depressed in-patients are satisfactory and close to those obtained in depressed out-patients. The high sensitivity to change of the IDS-C may be an advantage for this scale as compared to the MADRS, especially in antidepressant drug trials.

  7. Id proteins: small molecules, mighty regulators.

    PubMed

    Ling, Flora; Kang, Bin; Sun, Xiao-Hong

    2014-01-01

    The family of inhibitor of differentiation (Id) proteins is a group of evolutionarily conserved molecules, which play important regulatory roles in organisms ranging from Drosophila to humans. Id proteins are small polypeptides harboring a helix-loop-helix (HLH) motif, which are best known to mediate dimerization with other basic HLH proteins, primarily E proteins. Because Id proteins do not possess the basic amino acids adjacent to the HLH motif necessary for DNA binding, Id proteins inhibit the function of E protein homodimers, as well as heterodimers between E proteins and tissue-specific bHLH proteins. However, Id proteins have also been shown to have E protein-independent functions. The Id genes are broadly but differentially expressed in a variety of cell types. Transcription of the Id genes is controlled by transcription factors such as C/EBPβ and Egr as well as by signaling pathways triggered by different stimuli, which include bone morphogenic proteins, cytokines, and ligands of T cell receptors. In general, Id proteins are capable of inhibiting the differentiation of progenitors of different cell types, promoting cell-cycle progression, delaying cellular senescence, and facilitating cell migration. These properties of Id proteins enable them to play significant roles in stem cell maintenance, vasculogenesis, tumorigenesis and metastasis, the development of the immune system, and energy metabolism. In this review, we intend to highlight the current understanding of the function of Id proteins and discuss gaps in our knowledge about the mechanisms whereby Id proteins exert their diverse effects in multiple cellular processes.

  8. A vulnerability tool for adapting water and aquatic resources to climate change and extremes on the Shoshone National Forest, Wyoming

    NASA Astrophysics Data System (ADS)

    Rice, J.; Joyce, L. A.; Armel, B.; Bevenger, G.; Zubic, R.

    2011-12-01

    Climate change introduces a significant challenge for land managers and decision makers managing the natural resources that provide many benefits from forests. These benefits include water for urban and agricultural uses, wildlife habitat, erosion and climate control, aquifer recharge, stream flows regulation, water temperature regulation, and cultural services such as outdoor recreation and aesthetic enjoyment. The Forest Service has responded to this challenge by developing a national strategy for responding to climate change (the National Roadmap for Responding to Climate Change, July 2010). In concert with this national strategy, the Forest Service's Westwide Climate Initiative has conducted 4 case studies on individual Forests in the western U.S to develop climate adaptation tools. Western National Forests are particularly vulnerable to climate change as they have high-mountain topography, diversity in climate and vegetation, large areas of water limited ecosystems, and increasing urbanization. Information about the vulnerability and capacity of resources to adapt to climate change and extremes is lacking. There is an urgent need to provide customized tools and synthesized local scale information about the impacts to resources from future climate change and extremes, as well as develop science based adaptation options and strategies in National Forest management and planning. The case study on the Shoshone National Forest has aligned its objectives with management needs by developing a climate extreme vulnerability tool that guides adaptation options development. The vulnerability tool determines the likely degree to which native Yellowstone cutthroat trout and water availability are susceptible to, or unable to cope with adverse effects of climate change extremes. We spatially categorize vulnerability for water and native trout resources using exposure, sensitivity, and adaptive capacity indicators that use minimum and maximum climate and GIS data. Results

  9. R&D100: IC ID

    SciTech Connect

    Hamlet, Jason; Pierson, Lyndon; Bauer, Todd

    2015-11-19

    Supply chain security to detect, deter, and prevent the counterfeiting of networked and stand-alone integrated circuits (ICs) is critical to cyber security. Sandia National Laboratory researchers have developed IC ID to leverage Physically Unclonable Functions (PUFs) and strong cryptographic authentication to create a unique fingerprint for each integrated circuit. IC ID assures the authenticity of ICs to prevent tampering or malicious substitution.

  10. AFRD WAREHOUSE, EAST SIDE DETAIL SHOWS CONNECTION OF LEANTO TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AFRD WAREHOUSE, EAST SIDE DETAIL SHOWS CONNECTION OF LEAN-TO TO WALL. FACING WEST. NOTE THE PROFILE OF THE METAL AWNING ON SOUTH SIDE. ELECTRICAL CONDUIT AND OTHER SERVICES PENETRATE WALL. POLE SECURED WITH TRIANGULAR BRACES AT CORNER IS COMMUNICATION POLE. - Minidoka Relocation Center Warehouse, 111 South Fir Street, Shoshone, Lincoln County, ID

  11. AFRD WAREHOUSE, WEST SIDE, FACING EAST. NOTE PLACEMENT OF METAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AFRD WAREHOUSE, WEST SIDE, FACING EAST. NOTE PLACEMENT OF METAL SIDING BETWEEN THE TWO HALVES OF THE SLIDING DOOR. ORIGINAL 8-PANE WINDOW HAS BEEN REPLACED BY A SINGLE PANE OF GLASS. CONCRETE BRICK CHIMNEY IS AT LEFT OF VIEW. - Minidoka Relocation Center Warehouse, 111 South Fir Street, Shoshone, Lincoln County, ID

  12. 2. BUNKER HILL LEAD SMELTER. VIEW IS FROM CIA TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. BUNKER HILL LEAD SMELTER. VIEW IS FROM CIA TO THE SOUTH. IN FOREGROUND, PLANT DRY, SLAG FUMING PLANT, BLAST FURNACE, SMELTER OFFICE, LEAD AND SILVER REFINERIES ARE VISIBLE, L. TO R. HIGH VELOCITY FLUE LEADS FROM LOWER PLANT TO BAG HOUSE AND STACKS AT TOP OF SMELTING FACILITY. - Bunker Hill Lead Smelter, Bradley Rail Siding, Kellogg, Shoshone County, ID

  13. 1. BUNKER HILL LEAD SMELTER. VIEW IS FROM CENTRAL IMPOUNDMENT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. BUNKER HILL LEAD SMELTER. VIEW IS FROM CENTRAL IMPOUNDMENT AREA LOOKING SOUTH. PLANT DRY IS IN CENTER FOREGROUND, SLAG FUMING PLANT IS IN RIGHT FOREGROUND, AND BAG HOUSE IS IN RIGHT BACKGROUND. VARIOUS PLANT STACKS ARE ALSO VISIBLE. - Bunker Hill Lead Smelter, Bradley Rail Siding, Kellogg, Shoshone County, ID

  14. 1. NORTH IDAHO PHOSPHATE COMPANY PLANTS. VIEW IS TO THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. NORTH IDAHO PHOSPHATE COMPANY PLANTS. VIEW IS TO THE NORTHEAST, WITH THE SHIPPING AND STORAGE WAREHOUSE, AMMONIUM PHOSPHATE FERTILIZER PLANT, AND PHOSPHORIC ACID PLANT APPEARING IN SUCCESSION DOWN GOVERNMENT GULCH. - North Idaho Phosphate Company, Silver King Community, Kellogg, Shoshone County, ID

  15. 2. PHOSPHATE UNLOADING BUILDING. VIEW IS TO THE NORTH. THIS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. PHOSPHATE UNLOADING BUILDING. VIEW IS TO THE NORTH. THIS STRUCTURE WAS RELOCATED TO THE SOUTH OF ITS ORIGINAL SITE IN 1993 FOR USE AS A DECONTAMINATION FACILITY WITHIN THE BUNKER HILL SUPERFUND SITE. - North Idaho Phosphate Company, Silver King Community, Kellogg, Shoshone County, ID

  16. AFRD WAREHOUSE, WEST SIDE DETAIL OF ALTERED SLIDING DOORS, FACING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AFRD WAREHOUSE, WEST SIDE DETAIL OF ALTERED SLIDING DOORS, FACING EAST. WEATHER COVER OVER RAIL IS ORIGINAL. SHEET METAL SIDING HAS BEEN INSERTED BETWEEN TWO HALVES OF SLIDING DOORS. - Minidoka Relocation Center Warehouse, 111 South Fir Street, Shoshone, Lincoln County, ID

  17. Health assessment for Bunker Hill Mining and Metallurgical Complex, Kellogg, Shoshone County, Idaho, Region 10. CERCLIS No. IDD048340921. Addenda. Preliminary report

    SciTech Connect

    Not Available

    1989-10-10

    The Bunker Hill site is listed, by the U.S. Environmental Protection Agency (EPA), on the National Priorities List (NPL). The 21 square-mile site includes the Bunker Hill mining and smelting complexes and the communities of Pinehurst, Page, Smelterville, Kellog, and Wardner, Idaho. Mining and smelting operations have occurred in the area (Silver Valley) since the 1880's. The Bunker Hill smelter discontinued operation in 1982. The former milling and smelting operation at the Bunker Hill Complex has left behind contaminated soils and deposits of slag, mine tailings, and other process residuals. Based upon the information reviewed, ATSDR has concluded that this site is of public health concern because of the risk to human health caused by the probable human exposure to hazardous substances at concentrations that may result in adverse health effects. Human exposure to heavy metals is probably occurring via ingestion, dermal, or inhalation exposure to contaminated surface soils, mine wastes and tailings, surface waters, or contaminated foodstuffs.

  18. Id-1 and Id-2 genes and products as markers of epithelial cancer

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2008-09-30

    A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

  19. Id-1 and Id-2 genes and products as markers of epithelial cancer

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2011-10-04

    A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

  20. Estimation of unsaturated zone traveltimes for Rainier Mesa and Shoshone Mountain, Nevada Test Site, Nevada, using a source-responsive preferential-flow model

    SciTech Connect

    Brian A. Ebel; John R. Nimmo

    2009-09-11

    Traveltimes for contaminant transport by water from a point in the unsaturated zone to the saturated zone are a concern at Rainier Mesa and Shoshone Mountain in the Nevada Test Site, Nevada. Where nuclear tests were conducted in the unsaturated zone, contaminants must traverse hundreds of meters of variably saturated rock before they enter the saturated zone in the carbonate rock, where the regional groundwater system has the potential to carry them substantial distances to a location of concern. The unsaturated-zone portion of the contaminant transport path may cause a significant delay, in addition to the time required to travel within the saturated zone, and thus may be important in the overall evaluation of the potential hazard from contamination. Downward contaminant transport through the unsaturated zone occurs through various processes and pathways; this can lead to a broad distribution of contaminant traveltimes, including exceedingly slow and unexpectedly fast extremes. Though the bulk of mobile contaminant arrives between the time-scale end members, the fastest contaminant transport speed, in other words the speed determined by the combination of possible processes and pathways that would bring a measureable quantity of contaminant to the aquifer in the shortest time, carries particular regulatory significance because of its relevance in formulating the most conservative hazard-prevention scenarios. Unsaturated-zone flow is usually modeled as a diffusive process responding to gravity and pressure gradients as mediated by the unsaturated hydraulic properties of the materials traversed. The mathematical formulation of the diffuse-flow concept is known as Richards' equation, which when coupled to a solute transport equation, such as the advection-dispersion equation, provides a framework to simulate contaminant migration in the unsaturated zone. In recent decades awareness has increased that much fluid flow and contaminant transport within the unsaturated

  1. Estimation of Unsaturated Zone Traveltimes for Rainier Mesa and Shoshone Mountain, Nevada Test Site, Nevada, Using a Source-Responsive Preferential-Flow Model

    USGS Publications Warehouse

    Ebel, Brian A.; Nimmo, John R.

    2009-01-01

    Traveltimes for contaminant transport by water from a point in the unsaturated zone to the saturated zone are a concern at Rainier Mesa and Shoshone Mountain in the Nevada Test Site, Nevada. Where nuclear tests were conducted in the unsaturated zone, contaminants must traverse hundreds of meters of variably saturated rock before they enter the saturated zone in the carbonate rock, where the regional groundwater system has the potential to carry them substantial distances to a location of concern. The unsaturated-zone portion of the contaminant transport path may cause a significant delay, in addition to the time required to travel within the saturated zone, and thus may be important in the overall evaluation of the potential hazard from contamination. Downward contaminant transport through the unsaturated zone occurs through various processes and pathways; this can lead to a broad distribution of contaminant traveltimes, including exceedingly slow and unexpectedly fast extremes. Though the bulk of mobile contaminant arrives between the time-scale end members, the fastest contaminant transport speed, in other words the speed determined by the combination of possible processes and pathways that would bring a measureable quantity of contaminant to the aquifer in the shortest time, carries particular regulatory significance because of its relevance in formulating the most conservative hazard-prevention scenarios. Unsaturated-zone flow is usually modeled as a diffusive process responding to gravity and pressure gradients as mediated by the unsaturated hydraulic properties of the materials traversed. The mathematical formulation of the diffuse-flow concept is known as Richards' equation, which when coupled to a solute transport equation, such as the advection-dispersion equation, provides a framework to simulate contaminant migration in the unsaturated zone. In recent decades awareness has increased that much fluid flow and contaminant transport within the unsaturated

  2. Selection of starter cultures for idli batter fermentation and their effect on quality of idlis.

    PubMed

    Sridevi, J; Halami, Prakash M; Vijayendra, S V N

    2010-10-01

    Idli batter samples were prepared using lactic starter cultures like Pediococcus pentosaceus (Pp), Enterococcus faecium MTCC 5153 (Ef), Ent. faecium (IB2 Ef-IB2), individually, along with the yeast culture, Candida versatilis (Cv). Idli batter prepared using Ef and Ef-IB2 cultures gave better results, when evaluated for the rise in batter volume (80 ml), level of CO2 production (23.8%), titratable acidity 2.4-3.5% (lactic acid) and pH 4.3-4.4. Storage stability of batter made with selected starter cultures was determined by analyzing the idlis prepared using the batter stored for 1 and 5 days for texture, nutrient composition and sensory quality. Slight variations in the results were seen among the idlis of different combination of cultures, whereas these results are better than that of the idlis made using naturally fermented idli batter. Sensory profile of idlis prepared using starter cultures had a higher score (3.9-4.4) compared to the control (3.6) for overall acceptability.

  3. Selection of starter cultures for idli batter fermentation and their effect on quality of idlis.

    PubMed

    Sridevi, J; Halami, Prakash M; Vijayendra, S V N

    2010-10-01

    Idli batter samples were prepared using lactic starter cultures like Pediococcus pentosaceus (Pp), Enterococcus faecium MTCC 5153 (Ef), Ent. faecium (IB2 Ef-IB2), individually, along with the yeast culture, Candida versatilis (Cv). Idli batter prepared using Ef and Ef-IB2 cultures gave better results, when evaluated for the rise in batter volume (80 ml), level of CO2 production (23.8%), titratable acidity 2.4-3.5% (lactic acid) and pH 4.3-4.4. Storage stability of batter made with selected starter cultures was determined by analyzing the idlis prepared using the batter stored for 1 and 5 days for texture, nutrient composition and sensory quality. Slight variations in the results were seen among the idlis of different combination of cultures, whereas these results are better than that of the idlis made using naturally fermented idli batter. Sensory profile of idlis prepared using starter cultures had a higher score (3.9-4.4) compared to the control (3.6) for overall acceptability. PMID:23572685

  4. What Is "Real-World" ID Anyway?

    ERIC Educational Resources Information Center

    Hardre, Patricia L.

    2013-01-01

    The term "real-world ID" is commonly heard at professional conferences, but its meaning is assumed rather than defined. Unless we examine the meanings and implications of rhetoric of the field, we risk error in presenting ourselves, and we risk derailing the progress of our profession. This article examines the term "real world" as applied to…

  5. 76 FR 31388 - Idaho Disaster #ID-00014

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Idaho Disaster ID-00014 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  6. 75 FR 45682 - Idaho Disaster #ID-00010

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Idaho Disaster ID-00010 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  7. Characterization of Inhibitor of differentiation (Id) proteins in human cornea.

    PubMed

    Mohan, Rajiv R; Morgan, Brandie R; Anumanthan, Govindaraj; Sharma, Ajay; Chaurasia, Shyam S; Rieger, Frank G

    2016-05-01

    Inhibitor of differentiation (Id) proteins are DNA-binding transcription factors involved in cellular proliferation, migration, inflammation, angiogenesis and fibrosis. However, their expression and role in the cornea is unknown. The present study was undertaken to characterize the expression of Id proteins and their interactions with the pro-fibrotic cytokine Transforming Growth Factor β1 (TGFβ1) and anti-fibrotic cytokine, bone morphogenic protein 7 (BMP7) in human cornea. Human donor corneas procured from Eye Bank were used. Id proteins were localized in human corneal sections using immunofluorescence. Primary cultures of human corneal fibroblasts (HCF) were established and treated with either TGFβ1 (5 ng/ml) or BMP7 (10 ng/ml) for 24 h in serum free medium. Expression of Id's in response to TGFβ1, BMP7 and TGFβ1 + BMP7 was analyzed by quantitative real time PCR (qRT-PCR) and western blot analysis. Id1 and Id2 proteins were ubiquitously expressed in the epithelial cells and stromal keratocytes in human cornea. The Id1 was localized to the basal epithelial cells as seen by immunohistochemistry. HCF expressed all known mammalian Id genes (Id1-Id4). In addition, Id1 and Id2 are selectively expressed in HCF. Treatment of human recombinant TGFβ1 (5 ng/ml) to serum-starved HCF showed a significant increase in Id genes (Id1, Id2 and Id4) at 2 h time point compared to BMP7 treatment, which showed time dependent increase in the expression of Id1-Id3 at 24-48 h. Combined treatment with TGFβ1 + BMP7 to HCF showed a significant increase in Id1 transcript and an increasing trend in Id3 and Id4 expression. The results of this study suggest that Id family of genes (Id1-Id4) are localized in the human cornea and expressed in the corneal fibroblasts. Also, Id's were differentially regulated with TGFβ1 and/or BMP7 in a time dependent manner and might serve as a therapeutic target in corneal fibrosis.

  8. 75 FR 81269 - Ward Transformer Superfund Site Raleigh, Wake County, NC; Notice of Settlements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-27

    ... AGENCY Ward Transformer Superfund Site Raleigh, Wake County, NC; Notice of Settlements AGENCY... Ward Transformer Superfund Site located in Raleigh, Wake County, North Carolina for publication. DATES... your comments, identified by Docket ID No. EPA-RO4- SFUND-2010-1053 or Site name Ward...

  9. 76 FR 13616 - Picayune Wood Treating Site Picayune, Pearl River County, MS; Notice of Settlement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... AGENCY Picayune Wood Treating Site Picayune, Pearl River County, MS; Notice of Settlement AGENCY... Picayune Wood Treating Site located in Picayune, Pearl River County, Mississippi for publication. DATES..., identified by Docket ID No. EPA-RO4- SFUND-2011-0201 or Site name Picayune Wood Treating Superfund Site...

  10. 76 FR 14970 - National Starch and Chemical Company, Salisbury, Rowan County, NC; Notice of Settlement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-18

    ... AGENCY National Starch and Chemical Company, Salisbury, Rowan County, NC; Notice of Settlement AGENCY... National Starch and Chemical Company Site located in Mobile, Mobile County, Alabama for publication. DATES..., identified by Docket ID No. EPA-RO4- SFUND-2011-0278 or Site name National Starch and Chemical...

  11. Evidence for large-magnitude, post-Eocene extension in the northern Shoshone Range, Nevada, and its implications for Carlin-type gold deposits in the lower plate of the Roberts Mountains allochthon

    USGS Publications Warehouse

    Colgan, Joseph P.; Henry, Christopher D.; John, David A.

    2014-01-01

    The northern Shoshone and Toiyabe Ranges in north-central Nevada expose numerous areas of mineralized Paleozoic rock, including major Carlin-type gold deposits at Pipeline and Cortez. Paleozoic rocks in these areas were previously interpreted to have undergone negligible postmineralization extension and tilting, but here we present new data that suggest major post-Eocene extension along west-dipping normal faults. Tertiary rocks in the northern Shoshone Range crop out in two W-NW–trending belts that locally overlie and intrude highly deformed Lower Paleozoic rocks of the Roberts Mountains allochthon. Tertiary exposures in the more extensive, northern belt were interpreted as subvertical breccia pipes (intrusions), but new field data indicate that these “pipes” consist of a 35.8 Ma densely welded dacitic ash flow tuff (informally named the tuff of Mount Lewis) interbedded with sandstones and coarse volcaniclastic deposits. Both tuff and sedimentary rocks strike N-S and dip 30° to 70° E; the steeply dipping compaction foliation in the tuffs was interpreted as subvertical flow foliation in breccia pipes. The southern belt along Mill Creek, previously mapped as undivided welded tuff, includes the tuff of Cove mine (34.4 Ma) and unit B of the Bates Mountain Tuff (30.6 Ma). These tuffs dip 30° to 50° east, suggesting that their west-dipping contacts with underlying Paleozoic rocks (previously mapped as depositional) are normal faults. Tertiary rocks in both belts were deposited on Paleozoic basement and none appear to be breccia pipes. We infer that their present east tilt is due to extension on west-dipping normal faults. Some of these faults may be the northern strands of middle Miocene (ca. 16 Ma) faults that cut and tilted the 34.0 Ma Caetano caldera ~40° east in the central Shoshone Range (

  12. Geologic Surface Effects of Underground Nuclear Testing, Buckboard Mesa, Climax Stock, Dome Mountain, Frenchman Flat, Rainier/Aqueduct Mesa, and Shoshone Mountain, Nevada Test Site, Nevada

    USGS Publications Warehouse

    Grasso, Dennis N.

    2003-01-01

    Surface effects maps were produced for 72 of 89 underground detonations conducted at the Frenchman Flat, Rainier Mesa and Aqueduct Mesa, Climax Stock, Shoshone Mountain, Buckboard Mesa, and Dome Mountain testing areas of the Nevada Test Site between August 10, 1957 (Saturn detonation, Area 12) and September 18, 1992 (Hunters Trophy detonation, Area 12). The ?Other Areas? Surface Effects Map Database, which was used to construct the maps shown in this report, contains digital reproductions of these original maps. The database is provided in both ArcGIS (v. 8.2) geodatabase format and ArcView (v. 3.2) shapefile format. This database contains sinks, cracks, faults, and other surface effects having a combined (cumulative) length of 136.38 km (84.74 mi). In GIS digital format, the user can view all surface effects maps simultaneously, select and view the surface effects of one or more sites of interest, or view specific surface effects by area or site. Three map layers comprise the database. They are: (1) the surface effects maps layer (oase_n27f), (2) the bar symbols layer (oase_bar_n27f), and (3) the ball symbols layer (oase_ball_n27f). Additionally, an annotation layer, named 'Ball_and_Bar_Labels,' and a polygon features layer, named 'Area12_features_poly_n27f,' are contained in the geodatabase version of the database. The annotation layer automatically labels all 295 ball-and-bar symbols shown on these maps. The polygon features layer displays areas of ground disturbances, such as rock spall and disturbed ground caused by the detonations. Shapefile versions of the polygon features layer in Nevada State Plane and Universal Transverse Mercator projections, named 'area12_features_poly_n27f.shp' and 'area12_features_poly_u83m.shp,' are also provided in the archive.

  13. Remembering one's ID/E-ntity: E/ID protein regulation of T cell memory.

    PubMed

    Omilusik, Kyla D; Shaw, Laura A; Goldrath, Ananda W

    2013-10-01

    Upon infection, CD8(+) T cells proliferate and differentiate into armed effector cells capable of eliminating the assaulting pathogen. Although the majority of the antigen-specific T cells will die as the immune response wanes, a few will survive indefinitely to establish the memory population and provide long-lived protection against reinfection. E protein transcription factors and their inhibitors, ID proteins, operate to balance expression of genes that control CD8(+) T cell differentiation through this process. Here, we discuss the role of ID2 and ID3 in promoting the generation and survival of effector and memory populations, particularly highlighting their reciprocal roles in shaping the CD8(+) T cell response unique to the inflammatory milieu. We further examine this coordinated control of gene expression in the context of additional transcription factors within the transcriptional network that programs CD8(+) effector and memory T cell differentiation.

  14. Airless drying -- Developments since IDS'94

    SciTech Connect

    Stubbing, T.J.

    1999-09-01

    Since its introduction to IDS'94 delegates, significant progress has been made with the development of airless drying technology. The ceramic industry internationally is beginning to benefit from both the energy use and drying time reductions it achieves, while on the basis of further theoretical work carried out since 1993 other industries, including the bioenergy sector, should also soon begin to exploit its advantages. As global warming becomes a reality and oil reserves decline, superheated steam drying and gasification of biomass will contribute to the mitigation of those problems.

  15. The transcriptional regulators Id2 and Id3 control the formation of distinct memory CD8+ T cell subsets.

    PubMed

    Yang, Cliff Y; Best, J Adam; Knell, Jamie; Yang, Edward; Sheridan, Alison D; Jesionek, Adam K; Li, Haiyan S; Rivera, Richard R; Lind, Kristin Camfield; D'Cruz, Louise M; Watowich, Stephanie S; Murre, Cornelis; Goldrath, Ananda W

    2011-11-06

    During infection, naive CD8(+) T cells differentiate into effector cells, which are armed to eliminate pathogens, and memory cells, which are poised to protect against reinfection. The transcriptional program that regulates terminal differentiation into short-lived effector-memory versus long-lived memory cells is not clearly defined. Through the use of mice expressing reporters for the DNA-binding inhibitors Id2 and Id3, we identified Id3(hi) precursors of long-lived memory cells before the peak of T cell population expansion or upregulation of cell-surface receptors that indicate memory potential. Deficiency in Id2 or Id3 resulted in loss of distinct CD8(+) effector and memory populations, which demonstrated unique roles for these inhibitors of E-protein transcription factors. Furthermore, cytokines altered the expression of Id2 and Id3 differently, which provides insight into how external cues influence gene expression.

  16. To ID or Not to ID? Changes in Classification Rates of Intellectual Disability Using "DSM-5"

    ERIC Educational Resources Information Center

    Papazoglou, Aimilia; Jacobson, Lisa A.; McCabe, Marie; Kaufmann, Walter; Zabel, T. Andrew

    2014-01-01

    The "Diagnostic and Statistical Manual of Mental Disorders-Fifth Edition" ("DSM-5") diagnostic criteria for intellectual disability (ID) include a change to the definition of adaptive impairment. New criteria require impairment in one adaptive domain rather than two or more skill areas. The authors examined the diagnostic…

  17. Low Physical Fitness Levels in Older Adults with ID: Results of the HA-ID Study

    ERIC Educational Resources Information Center

    Hilgenkamp, Thessa I. M.; van Wijck, Ruud; Evenhuis, Heleen M.

    2012-01-01

    Physical fitness is as important to aging adults with ID as in the general population, but to date, the physical fitness levels of this group are unknown. Comfortable walking speed, muscle strength (grip strength), muscle endurance (30 s Chair stand) and cardiorespiratory endurance (10 m incremental shuttle walking test) were tested in a sample of…

  18. Transcriptional regulator Id2 mediates CD8+ T cell immunity.

    PubMed

    Cannarile, Michael A; Lind, Nicholas A; Rivera, Richard; Sheridan, Alison D; Camfield, Kristin A; Wu, Bei Bei; Cheung, Kitty P; Ding, Zhaoqing; Goldrath, Ananda W

    2006-12-01

    Transcriptional programs that initiate and sustain the proliferation, differentiation and survival of CD8(+) T cells during immune responses are not completely understood. Here we show that inhibitor of DNA binding 2 (Id2), an antagonist of E protein transcription factors, was upregulated in CD8(+) T cells during infection and that expression of Id2 was maintained in memory CD8(+) T cells. Although Id2-deficient naive CD8(+) T cells recognized antigen and proliferated normally early after infection, effector CD8(+) T cells did not accumulate because the cells were highly susceptible to apoptosis. Id2-deficient CD8(+) T cells responding to infection had changes in the expression of genes that influence survival and had altered memory formation. Our data emphasize the importance of Id2 in regulating gene expression by CD8(+) T cells and the magnitude of effector responses, suggesting a mechanism involving Id protein- and E protein-mediated survival and differentiation of mature T cells.

  19. Evaluation of the chromogenic agar chromID C. difficile.

    PubMed

    Eckert, Catherine; Burghoffer, Béatrice; Lalande, Valérie; Barbut, Frederic

    2013-03-01

    Three selective media (chromID C. difficile agar, taurocholate cycloserine cefoxitin agar [TCCA; homemade], and CLO medium) were compared from 406 stool samples of patients suspected of having Clostridium difficile infection. The sensitivities of chromID C. difficile agar at 24 h and 48 h, CLO medium, and TCCA were 74.1%, 87%, 85.2%, and 70.4%, respectively.

  20. Id4 Marks Spermatogonial Stem Cells in the Mouse Testis.

    PubMed

    Sun, Feng; Xu, Qing; Zhao, Danfeng; Degui Chen, Charlie

    2015-01-01

    Mammalian spermatogenesis is a classic adult stems cell-dependent process, supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). However, the identification of SSCs and elucidation of their behaviors in undisturbed testis has long been a big challenge. Here, we generated a knock-in mouse model, Id4-2A-CreERT2-2A-tdTomato, which allowed us to mark Id4-expressing (Id4(+)) cells at different time points in situ and track their behaviors across distinct developmental stages during steady-state and regenerating spermatogenesis. We found that Id4(+) cells continue to produce spermatogonia, spermatocytes and sperm in mouse testis, showing they are capable of self-renewal and have differentiation potential. Consistent with these findings, ablation of Id4(+) cells in mice results in a loss of spermatogenesis. Furthermore, developmental fate mapping reveals that Id4(+) SSCs originate from neonate Id4(+) gonocytes. Therefore, our results indicate that Id4 marks spermatogonial stem cells in the mouse testis.

  1. ID Model Construction and Validation: A Multiple Intelligences Case

    ERIC Educational Resources Information Center

    Tracey, Monica W.; Richey, Rita C.

    2007-01-01

    This is a report of a developmental research study that aimed to construct and validate an instructional design (ID) model that incorporates the theory and practice of multiple intelligences (MI). The study consisted of three phases. In phase one, the theoretical foundations of multiple Intelligences and ID were examined to guide the development…

  2. 75 FR 12974 - Establishment of Class E Airspace; Hailey, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ... E airspace at Hailey, ID, to accommodate aircraft using the Area Navigation (RNAV) Global Positioning System (GPS) Standard Instrument Approach Procedure (SIAP) at Friedman Memorial Airport. This will... Register a notice of proposed rulemaking to establish additional controlled airspace at Hailey, ID (74...

  3. An ID2-dependent mechanism for VHL inactivation in cancer.

    PubMed

    Lee, Sang Bae; Frattini, Veronique; Bansal, Mukesh; Castano, Angelica M; Sherman, Dan; Hutchinson, Keino; Bruce, Jeffrey N; Califano, Andrea; Liu, Guangchao; Cardozo, Timothy; Iavarone, Antonio; Lasorella, Anna

    2016-01-14

    Mechanisms that maintain cancer stem cells are crucial to tumour progression. The ID2 protein supports cancer hallmarks including the cancer stem cell state. HIFα transcription factors, most notably HIF2α (also known as EPAS1), are expressed in and required for maintenance of cancer stem cells (CSCs). However, the pathways that are engaged by ID2 or drive HIF2α accumulation in CSCs have remained unclear. Here we report that DYRK1A and DYRK1B kinases phosphorylate ID2 on threonine 27 (Thr27). Hypoxia downregulates this phosphorylation via inactivation of DYRK1A and DYRK1B. The activity of these kinases is stimulated in normoxia by the oxygen-sensing prolyl hydroxylase PHD1 (also known as EGLN2). ID2 binds to the VHL ubiquitin ligase complex, displaces VHL-associated Cullin 2, and impairs HIF2α ubiquitylation and degradation. Phosphorylation of Thr27 of ID2 by DYRK1 blocks ID2-VHL interaction and preserves HIF2α ubiquitylation. In glioblastoma, ID2 positively modulates HIF2α activity. Conversely, elevated expression of DYRK1 phosphorylates Thr27 of ID2, leading to HIF2α destabilization, loss of glioma stemness, inhibition of tumour growth, and a more favourable outcome for patients with glioblastoma. PMID:26735018

  4. A Contextual Model for Identity Management (IdM) Interfaces

    ERIC Educational Resources Information Center

    Fuller, Nathaniel J.

    2014-01-01

    The usability of Identity Management (IdM) systems is highly dependent upon design that simplifies the processes of identification, authentication, and authorization. Recent findings reveal two critical problems that degrade IdM usability: (1) unfeasible techniques for managing various digital identifiers, and (2) ambiguous security interfaces.…

  5. DRAFT LANDSAT DATA MOSAIC: MONTGOMERY COUNTY, TEXAS; HARRIS COUNTY, TEXAS; FORT BEND COUNTY, TEXAS; BRAZORIA COUNTY, TEXAS; GALVESTON COUNTY, TEXAS

    EPA Science Inventory

    This is a draft Landsat Data Mosaic, which contains remote sensing information for Montgomery County, Texas Harris County, Texas Fort Bend County, Texas Brazoria County, Texas Galveston County, and Texas Imagery dates on the following dates: October 6, 1999 and September 29, 200...

  6. 4. BUNKER HILL LEAD SMELTER. VIEW IS FROM RIDGE ABOVE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. BUNKER HILL LEAD SMELTER. VIEW IS FROM RIDGE ABOVE GOVERNMENT GULCH LOOKING TO THE EAST. IN THE RIGHT MID GROUND, CARPENTER SHOP BUILDINGS AND FRAMING SHEDS ARE VISIBLE. THE BACKGROUND FACILITIES VISIBLE FROM L. TO R. ARE: SMELTER OFFICE, REFINERIES, SLAG FUMING STACKS, HIGH VELOCITY FLUE, BAG HOUSE, 200-FOOT STACK, AND 715-FOOT STACK. - Bunker Hill Lead Smelter, Bradley Rail Siding, Kellogg, Shoshone County, ID

  7. 32 CFR 161.8 - ID card life-cycle roles and responsibilities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 1 2014-07-01 2014-07-01 false ID card life-cycle roles and responsibilities... IDENTIFICATION (ID) CARDS FOR MEMBERS OF THE UNIFORMED SERVICES, THEIR DEPENDENTS, AND OTHER ELIGIBLE INDIVIDUALS DoD Identification (ID) Cards: ID Card Life-Cycle § 161.8 ID card life-cycle roles...

  8. 3-D RESERVOIR AND STOCHASTIC FRACTURE NETWORK MODELING FOR ENHANCED OIL RECOVERY, CIRCLE RIDGE PHOSPHORIA/TENSLEEP RESERVOIR, WIND RIVER RESERVATION, ARAPAHO AND SHOSHONE TRIBES, WYOMING

    SciTech Connect

    Paul La Pointe; Jan Hermanson; Robert Parney; Thorsten Eiben; Mike Dunleavy; Ken Steele; John Whitney; Darrell Eubanks; Roger Straub

    2002-11-18

    This report describes the results made in fulfillment of contract DE-FG26-00BC15190, ''3-D Reservoir and Stochastic Fracture Network Modeling for Enhanced Oil Recovery, Circle Ridge Phosphoria/Tensleep Reservoir, Wind River Reservation, Arapaho and Shoshone Tribes, Wyoming''. The goal of this project is to improve the recovery of oil from the Tensleep and Phosphoria Formations in Circle Ridge Oilfield, located on the Wind River Reservation in Wyoming, through an innovative integration of matrix characterization, structural reconstruction, and the characterization of the fracturing in the reservoir through the use of discrete fracture network models. Fields in which natural fractures dominate reservoir permeability, such as the Circle Ridge Field, often experience sub-optimal recovery when recovery processes are designed and implemented that do not take advantage of the fracture systems. For example, a conventional waterflood in a main structural block of the Field was implemented and later suspended due to unattractive results. It is estimated that somewhere less than 20% of the OOIP in the Circle Ridge Field have been recovered after more than 50 years' production. Marathon Oil Company identified the Circle Ridge Field as an attractive candidate for several advanced IOR processes that explicitly take advantage of the natural fracture system. These processes require knowledge of the distribution of matrix porosity, permeability and oil saturations; and understanding of where fracturing is likely to be well-developed or poorly developed; how the fracturing may compartmentalize the reservoir; and how smaller, relatively untested subthrust fault blocks may be connected to the main overthrust block. For this reason, the project focused on improving knowledge of the matrix properties, the fault block architecture and to develop a model that could be used to predict fracture intensity, orientation and fluid flow/connectivity properties. Knowledge of matrix properties was

  9. Id-1 and Id-2 genes and products as therapeutic targets for treatment of breast cancer and other types of carcinoma

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2014-09-30

    A method for treatment and amelioration of breast, cervical, ovarian, endometrial, squamous cells, prostate cancer and melanoma in a patient comprising targeting Id-1 or Id-2 gene expression with a delivery vehicle comprising a product which modulates Id-1 or Id-2 expression.

  10. Biometric authentication for ID cards with hologram watermarks

    NASA Astrophysics Data System (ADS)

    Croce Ferri, Lucilla; Mayerhoefer, Astrid; Frank, Marcus; Vielhauer, Claus; Steinmetz, Ralf

    2002-04-01

    We present an analysis of a new technique for the authentication of ID cardholders, based on the integration of a biometrics-based authentication system with digital watermarks. The chosen biometric authentication method is the dynamic signature verification of the ID cardholder, while we use a specific integrity watermark technique we developed, called Hologram Watermark, to embed and retrieve the off-line data onto an ID card. We take advantage of the fact, that two static biometric features (images of the user's face and signature) are already integral part of ID cards for manual verification and extent the stored biometric information by embedding on-line handwriting features of the signature as holographic watermarks in the overall image information of an ID card. Manipulation of any of the image information can be detected and will further disallow biometric verification of the forger. The Hologram Watermark technique produces content-related data using computer-generated hologram coding techniques. These data are embedded with watermarking techniques into the personal data printed on an ID card. The content-related data in this specific application are the dynamic features of the cardholder's signature. The main goal of this paper is to analyze the suitability of dynamic signature verification in combination with the Hologram Watermark technique, to facilitate automated user authentication based on information transparently embedded in ID cards.

  11. New security system for ID certificates in IT society

    NASA Astrophysics Data System (ADS)

    Nagashima, Hisato; Saito, Kazuharu

    2004-06-01

    This paper introduces a new security solution regarding security documents with secure unique information. Our newly proposed security measure enables outputting ID documents by commercially available printer. On this basis, a citizen can apply and accept his ID certificates to and from Issuing Authority via website. A unique gradational latent image emerges if a third party authenticates it under Infrared ray. The principle of this new measure lies in the complicated microstructure generated by our specially designed software. It is understood that its security feature based on secure software and wide applicability for commercially available printers show profound potentiality to construct new security system for ID documents in IT society.

  12. MicroRNAs and intellectual disability (ID) in Down syndrome, X-linked ID, and Fragile X syndrome

    PubMed Central

    Siew, Wei-Hong; Tan, Kai-Leng; Babaei, Maryam Abbaspour; Cheah, Pike-See; Ling, King-Hwa

    2013-01-01

    Intellectual disability (ID) is one of the many features manifested in various genetic syndromes leading to deficits in cognitive function among affected individuals. ID is a feature affected by polygenes and multiple environmental factors. It leads to a broad spectrum of affected clinical and behavioral characteristics among patients. Until now, the causative mechanism of ID is unknown and the progression of the condition is poorly understood. Advancement in technology and research had identified various genetic abnormalities and defects as the potential cause of ID. However, the link between these abnormalities with ID is remained inconclusive and the roles of many newly discovered genetic components such as non-coding RNAs have not been thoroughly investigated. In this review, we aim to consolidate and assimilate the latest development and findings on a class of small non-coding RNAs known as microRNAs (miRNAs) involvement in ID development and progression with special focus on Down syndrome (DS) and X-linked ID (XLID) [including Fragile X syndrome (FXS)]. PMID:23596395

  13. Scientists ID Key Fetal Cells Vulnerable to Zika

    MedlinePlus

    ... html Scientists ID Key Fetal Cells Vulnerable to Zika Lab study suggests possible mechanism for birth defects ... 29, 2016 (HealthDay News) -- The devastating mosquito-borne Zika virus can infect cells that play a role ...

  14. Study IDs 2 Zika Virus Proteins Linked to Microcephaly

    MedlinePlus

    ... gov/news/fullstory_160386.html Study IDs 2 Zika Virus Proteins Linked to Microcephaly Discovery might one ... say they've identified two proteins in the Zika virus linked to the severe birth defect microcephaly. ...

  15. IDS Combined Solution improvements between ITRF2008 and ITRF2013

    NASA Astrophysics Data System (ADS)

    Moreaux, Guilhem; Lemoine, Frank; Willis, Pascal; Capdeville, Hugues; Otten, Michiel; Stepanek, Petr; Kuzin, Sergei; Ferrage, Pascale

    2015-04-01

    In the context of ITRF 2013, the IDS Combination Center delivered to IERS weekly combined SINEX files from early January 1993 to late August 2014. These SINEX files which contain DORIS stations positions/velocities and Earth orientation parameters from DORIS data are based on the latest series of all of the 6 IDS Analysis Centers. The primary objective of this study is to present latest results from the DORIS contribution to ITRF2013. Then, comparisons with IDS contribution to ITRF2008 will address benefits of including both South Atlantic Anomaly corrected data and new DORIS missions (e.g. Jason-1, Jason-2, Cryosat-2) in the IDS combination. These comparisons will also emphasize application of DORIS ground antennas phase laws in the data processing and improved modeling of DORIS ground beacon frequency variations.

  16. Security analysis for biometric data in ID documents

    NASA Astrophysics Data System (ADS)

    Schimke, Sascha; Kiltz, Stefan; Vielhauer, Claus; Kalker, Ton

    2005-03-01

    In this paper we analyze chances and challenges with respect to the security of using biometrics in ID documents. We identify goals for ID documents, set by national and international authorities, and discuss the degree of security, which is obtainable with the inclusion of biometric into documents like passports. Starting from classical techniques for manual authentication of ID card holders, we expand our view towards automatic methods based on biometrics. We do so by reviewing different human biometric attributes by modality, as well as by discussing possible techniques for storing and handling the particular biometric data on the document. Further, we explore possible vulnerabilities of potential biometric passport systems. Based on the findings of that discussion we will expand upon two exemplary approaches for including digital biometric data in the context of ID documents and present potential risks attack scenarios along with technical aspects such as capacity and robustness.

  17. Genetics Experts Unite to I.D. Unknown Katrina Victims

    MedlinePlus

    ... Current Issue Past Issues Research News From NIH Genetics Experts Unite to I.D. Unknown Katrina Victims ... in the recent Advances in molecular biology and genetics," says team member Stephen Sherry, Ph.D., of ...

  18. 76 FR 33651 - Approval and Promulgation of Implementation Plans; ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... From the Federal Register Online via the Government Publishing Office ENVIRONMENTAL PROTECTION AGENCY 40 CFR Part 52 Approval and Promulgation of Implementation Plans; ID AGENCY: Environmental... includes new and revised rules which provide the Idaho Department of Environmental Quality (IDEQ)...

  19. A Good IDS Response Protocol of MANET Containment Strategies

    NASA Astrophysics Data System (ADS)

    Cheng, Bo-Chao; Chen, Huan; Tseng, Ryh-Yuh

    Much recent research concentrates on designing an Intrusion Detection System (IDS) to detect the misbehaviors of the malicious node in MANET with ad-hoc and mobility natures. However, without rapid and appropriate IDS response mechanisms performing follow-up management services, even the best IDS cannot achieve the desired primary goal of the incident response. A competent containment strategy is needed to limit the extent of an attack in the Incident Response Life Cycle. Inspired by the T-cell mechanisms in the human immune system, we propose an efficient MANET IDS response protocol (T-SecAODV) that can rapidly and accurately disseminate alerts of the malicious node attacks to other nodes so as to modify their AODV routing tables to isolate the malicious nodes. Simulations are conducted by the network simulator (Qualnet), and the experiment results indicate that T-SecAODV is able to spread alerts steadily while greatly reduce faulty rumors under simultaneous multiple malicious node attacks.

  20. Role of yeast domination in Indian idli batter fermentation.

    PubMed

    Soni, S K; Sandhu, D K

    1991-07-01

    Adding Saccharomyces cerevisiae, along with natural bacterial flora of the ingredients, was the best method for standardizing idli termentation in terms of improved organoleptic characteristics, leavening and nutritional constituents. PMID:24425138

  1. Biometrics and ID Cards — Enablers for Personal Security

    NASA Astrophysics Data System (ADS)

    Reisen, Andreas

    The electronic ID card is a modernization and security project of the German Government. On the one hand, the multifunctional card is intended to boost security and the convenience of e-government and e-business applications. On the other hand, the new biometric ID card should allow citizens to use it as a travel document in the Schengen area and for specific destinations outside the European Union also in the future.

  2. 77 FR 14417 - Notice of Temporary Closure on Public Lands in Gooding and Elmore Counties, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-09

    ... Burley Normal Fire Rehabilitation Plan: (1) Allow burned areas to re-establish a vegetative cover, which... species to become established; (3) allow remaining plants to recover from the effects of the fire; and (4... and crucial winter habitat for mule deer, which suffered significant losses in the Blair Fire. For...

  3. 75 FR 10811 - Notice of Application for Recordable Disclaimer of Interest in Lands, Gem County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-09

    ... filed with the Bureau of Land Management (BLM) by Cynthia L. Yee-Wallace, Attorney-at-Law, on behalf of... Act of 1976 (43 U.S.C. 1745), Cynthia L. Yee- Wallace, Attorney at Law, has filed an application...

  4. 77 FR 37702 - Grays Lake National Wildlife Refuge, Caribou and Bonneville Counties, ID; Comprehensive...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-22

    ... comments by August 21, 2012. We will announce opportunities for public input in local news media throughout..., wildlife observation and photography, and environmental education and interpretation. We will review...

  5. 76 FR 48877 - Kootenai National Wildlife Refuge, Boundary County, ID; Draft Comprehensive Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... Wildlife Refuge. We started this process through a notice in the Federal Register (74 FR 8102; February 23..., wildlife observation and photography, and environmental education and interpretation. We will review and... the public and obtain comments. The meetings were announced through local media outlets, on the...

  6. 78 FR 16523 - Deer Flat National Wildlife Refuge, Canyon, Payette, Owyhee, and Washington Counties, ID, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ..., and environmental education and interpretation. We will review and update the CCP at least every 15..., wildlife observation and photography, and environmental education and interpretation. The Draft CCP/EIS..., fishing, wildlife observation and photography, environmental education and interpretation as well...

  7. 75 FR 41232 - Deer Flat National Wildlife Refuge, Canyon, Owyhee, Payette, and Washington Counties, ID; Malheur...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... Fish and Wildlife Service Deer Flat National Wildlife Refuge, Canyon, Owyhee, Payette, and Washington...), intend to prepare a comprehensive conservation plan (CCP) for Deer Flat National Wildlife Refuge (Refuge....gov . Fax: Attn: Refuge Manager, (208) 467-1019. U.S. Mail: Refuge Manager, Deer Flat...

  8. 77 FR 51561 - Notice of Temporary Restriction Order for Skinny Dipper Hot Springs, Boise County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-24

    ... Bureau of Land Management Notice of Temporary Restriction Order for Skinny Dipper Hot Springs, Boise...: This serves as notice of a sunset-to-sunrise recreational use restriction of Skinny Dipper Hot Springs... Hot Springs, and the public lands in Lot 3, Section 25, T. 9 N., R.3 E., Boise Meridian, Boise...

  9. 77 FR 64352 - Notice of Temporary Closure of Public Lands in Custer County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-19

    ... wild horses and the time needed to move the horses to the gather corrals. The legal description of the.../st/en/prog/wild_horses_/gathers.html . Information will also be available by calling the Challis... ensure the safety of the contractors, employees, public and the horses while gather operations are...

  10. 77 FR 75654 - Notice of Temporary Closures on Public Lands in Owyhee County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-21

    ...) and Morley Nelson Snake River Birds of Prey National Conservation Area (NCA-BLM). Two roads will be... Roller, Morley Nelson Snake River Birds of Prey National Conservation Area Manager at 3948 Development..., Owyhee Field Manager. Patricia Roller, Morley Nelson Snake River Birds of Prey NCA Manager. BILLING...

  11. 78 FR 4869 - Notice of Intent To Collect Fees on Public Land in Clark County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-23

    ... (REA), the Bureau of Land Management (BLM) Upper Snake Field Office is proposing to collect a... implementing regulations, the Upper Snake Field Office is proposing to charge a group site reservation fee of... Council (RAC) and the BLM Idaho State Director. The BLM Upper Snake Field Office will provide final...

  12. 75 FR 57053 - Camas National Wildlife Refuge, Jefferson County, ID; Comprehensive Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-17

    ... refuge lies in the upper Snake River plain at approximately 4,800 feet in elevation. The refuge was... continually flooded the wetlands during the drier summer and fall months. The upper Snake River climate...

  13. 77 FR 16556 - Kootenai National Wildlife Refuge, Boundary County, ID; Final Comprehensive Conservation Plan and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-21

    ... a notice of intent in the Federal Register (74 FR 8102; February 23, 2009). We released the draft... Register (76 FR 48877; August 9, 2011). Kootenai NWR encompasses 2,774 acres along the lower Kootenai River... Administration Act. CCP Alternatives, Including Selected Alternative Our draft CCP/EA (76 FR 48877; August...

  14. 78 FR 23952 - Notice of Temporary Closure on Public Lands in Elmore County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-23

    ... Four Rivers Field Office, Bureau of Land Management (BLM). DATES: The Stout Fire closure will be in.... FOR FURTHER INFORMATION CONTACT: Larry Ridenhour, Four Rivers Field Office outdoor recreation planner...-fighting forces in the performance of their official duties; and persons with written authorization...

  15. 78 FR 20135 - Notice of Temporary Closure on Public Lands in Boise County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-03

    ... surrounding Skinny Dipper Hot Springs, near Banks, Idaho, that burned August 5, 2012. The affected public... recreating public around the hot springs due to fire damage, as a result of the loss of stabilizing vegetation upstream from the hot springs as identified in the Springs Fire Emergency Stabilization and...

  16. 75 FR 8744 - Notice of Proposed Issuance of Recordable Disclaimer of Interest; Jerome County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... with the former Minidoka Relocation Center where Japanese Americans were incarcerated during World War II. Section 313 of the Act makes several adjustments to the Historical Site boundary and gives...

  17. 75 FR 1026 - Lower Orogrande Project, Clearwater National Forest, Clearwater County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-08

    ... effects of improvements on watershed, vegetation, and wildlife habitat in the Lower Orogrande project area... watershed, vegetation, and wildlife habitat. Watershed improvements include: (1) Decommissioning 6 miles of... (1) conducting vegetation treatments to promote better successional stage balance. This action...

  18. Anti-ids in allergy: timeliness of a classic concept.

    PubMed

    Wallmann, Julia; Pali-Schöll, Isabella; Jensen-Jarolim, Erika

    2010-06-01

    Anti-idiotypic antibodies (anti-ids) are part of natural immune responses with regulatory capacity. Their effect on an antigen-specific, so-called Ab1 antibody response, is dependent on 1) the original antigen, which they mirror, being Ab2 antibodies, and 2) their isotype. In the case of IgE-mediated allergy, natural anti-ids against allergen-specific IgE represent internal images of allergen molecules. A key biologic feature of allergens is that they can crosslink IgE, expressed by B-lymphocytes or passively bound via high affinity receptors to effector cells, which renders cellular activation. Therefore, the IgE cross linking capability of anti-ids determines whether they dampen or enhance immediate-type hypersensitivity. Correspondingly to classic antiallergen blocking IgG antibodies, anti-ids may also interact with inhibitory FcγRIIb receptors and, thereby, down-regulate T(H)2-type inflammation. Anti-ids and other B-cell epitope mimetics, like mimotopes and DARPins, represent antigen surrogates, which can be used for vaccination. Intriguingly, they may induce antibody responses without activating potentially proinflammatory, antiallergen T-lymphocytes. Taken together, collective evidence suggests that anti-ids, although representing immunologic classics, are a timeless concept in allergology.

  19. Accelerated fermentation of 'idli' batter using soy residue okara.

    PubMed

    Rekha, C R; Vijayalakshmi, G

    2011-06-01

    The aim of this work was to reduce the natural fermentation period of 'idli' from the conventional 14 h to 10 h by adding underutilized okara for the preparation of 'idli'. Black gram was partially substituted with soy residue okara in the ratio of (1:1). After 14 h of natural fermentation, the pH and total acidity of control 'idli' batter was 4.51 and 0.64% and that of okara fortified 'idli' batter was 4.53 and 0.43%, respectively. The amount of CO2 released by the control and okara fortified batter was 19.7% and 33.6% respectively. The viable count of yeast and mold, lactics and mesophilic bacteria in control & okara batter increased with time reaching 9.00 & 10.34, 8.66 & 7.69, and 8.65 & 9.47 log10 cfu/g, respectively at the end of 10 h of natural fermentation. Okara fortified 'idli' was soft and spongy compared to control 'idli' .

  20. BioID Identification of Lamin-Associated Proteins.

    PubMed

    Mehus, Aaron A; Anderson, Ruthellen H; Roux, Kyle J

    2016-01-01

    A- and B-type lamins support the nuclear envelope, contribute to heterochromatin organization, and regulate a myriad of nuclear processes. The mechanisms by which lamins function in different cell types and the mechanisms by which lamin mutations cause over a dozen human diseases (laminopathies) remain unclear. The identification of proteins associated with lamins is likely to provide fundamental insight into these mechanisms. BioID (proximity-dependent biotin identification) is a unique and powerful method for identifying protein-protein and proximity-based interactions in living cells. BioID utilizes a mutant biotin ligase from bacteria that is fused to a protein of interest (bait). When expressed in living cells and stimulated with excess biotin, this BioID-fusion protein promiscuously biotinylates directly interacting and vicinal endogenous proteins. Following biotin-affinity capture, the biotinylated proteins can be identified using mass spectrometry. BioID thus enables screening for physiologically relevant protein associations that occur over time in living cells. BioID is applicable to insoluble proteins such as lamins that are often refractory to study by other methods and can identify weak and/or transient interactions. We discuss the use of BioID to elucidate novel lamin-interacting proteins and its applications in a broad range of biological systems, and provide detailed protocols to guide new applications.

  1. Accelerated fermentation of 'idli' batter using soy residue okara.

    PubMed

    Rekha, C R; Vijayalakshmi, G

    2011-06-01

    The aim of this work was to reduce the natural fermentation period of 'idli' from the conventional 14 h to 10 h by adding underutilized okara for the preparation of 'idli'. Black gram was partially substituted with soy residue okara in the ratio of (1:1). After 14 h of natural fermentation, the pH and total acidity of control 'idli' batter was 4.51 and 0.64% and that of okara fortified 'idli' batter was 4.53 and 0.43%, respectively. The amount of CO2 released by the control and okara fortified batter was 19.7% and 33.6% respectively. The viable count of yeast and mold, lactics and mesophilic bacteria in control & okara batter increased with time reaching 9.00 & 10.34, 8.66 & 7.69, and 8.65 & 9.47 log10 cfu/g, respectively at the end of 10 h of natural fermentation. Okara fortified 'idli' was soft and spongy compared to control 'idli' . PMID:23572755

  2. Bathymetry and capacity of Blackfoot Reservoir, Caribou County, Idaho, 2011

    USGS Publications Warehouse

    Wood, Molly S.; Skinner, Kenneth D.; Fosness, Ryan L.

    2012-01-01

    The U.S. Geological Survey (USGS), in cooperation with the Shoshone-Bannock Tribes, surveyed the bathymetry and selected above-water sections of Blackfoot Reservoir, Caribou County, Idaho, in 2011. Reservoir operators manage releases from Government Dam on Blackfoot Reservoir based on a stage-capacity relation developed about the time of dam construction in the early 1900s. Reservoir operation directly affects the amount of water that is available for irrigation of agricultural land on the Fort Hall Indian Reservation and surrounding areas. The USGS surveyed the below-water sections of the reservoir using a multibeam echosounder and real-time kinematic global positioning system (RTK-GPS) equipment at full reservoir pool in June 2011, covering elevations from 6,090 to 6,119 feet (ft) above the North American Vertical Datum of 1988 (NAVD 88). The USGS used data from a light detection and ranging (LiDAR) survey performed in 2000 to map reservoir bathymetry from 6,116 to 6,124 ft NAVD 88, which were mostly in depths too shallow to measure with the multibeam echosounder, and most of the above-water section of the reservoir (above 6,124 ft NAVD 88). Selected points and bank erosional features were surveyed by the USGS using RTK-GPS and a total station at low reservoir pool in September 2011 to supplement and verify the LiDAR data. The stage-capacity relation was revised and presented in a tabular format. The datasets show a 2.0-percent decrease in capacity from the original survey, due to sedimentation or differences in accuracy between surveys. A 1.3-percent error also was detected in the previously used capacity table and measured water-level elevation because of questionable reference elevation at monitoring stations near Government Dam. Reservoir capacity in 2011 at design maximum pool of 6,124 ft above NAVD 88 was 333,500 acre-ft.

  3. 76 FR 19096 - National Starch and Chemical Company, Salisbury, Rowan County, North Carolina; Notice of Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ... AGENCY National Starch and Chemical Company, Salisbury, Rowan County, North Carolina; Notice of... Liability Act (CERCLA), concerning the National Starch and Chemical Company Site located in Salisbury, Rowan..., identified by Docket ID No. EPA-RO4- SFUND-2011-0278 or Site name National Starch and Chemical...

  4. Multimodal biometrics for identity documents (MBioID).

    PubMed

    Dessimoz, Damien; Richiardi, Jonas; Champod, Christophe; Drygajlo, Andrzej

    2007-04-11

    The MBioID initiative has been set up to address the following germane question: What and how biometric technologies could be deployed in identity documents in the foreseeable future? This research effort proposes to look at current and future practices and systems of establishing and using biometric identity documents (IDs) and evaluate their effectiveness in large-scale developments. The first objective of the MBioID project is to present a review document establishing the current state-of-the-art related to the use of multimodal biometrics in an IDs application. This research report gives the main definitions, properties and the framework of use related to biometrics, an overview of the main standards developed in the biometric industry and standardisation organisations to ensure interoperability, as well as some of the legal framework and the issues associated to biometrics such as privacy and personal data protection. The state-of-the-art in terms of technological development is also summarised for a range of single biometric modalities (2D and 3D face, fingerprint, iris, on-line signature and speech), chosen according to ICAO recommendations and availabilities, and for various multimodal approaches. This paper gives a summary of the main elements of that report. The second objective of the MBioID project is to propose relevant acquisition and evaluation protocols for a large-scale deployment of biometric IDs. Combined with the protocols, a multimodal database will be acquired in a realistic way, in order to be as close as possible to a real biometric IDs deployment. In this paper, the issues and solutions related to the acquisition setup are briefly presented.

  5. Novel ID-based anti-collision approach for RFID

    NASA Astrophysics Data System (ADS)

    Zhang, De-Gan; Li, Wen-Bin

    2016-09-01

    Novel correlation ID-based (CID) anti-collision approach for RFID under the banner of the Internet of Things (IOT) has been presented in this paper. The key insights are as follows: according to the deterministic algorithms which are based on the binary search tree, we propose a method to increase the association between tags so that tags can initiatively send their own ID under certain trigger conditions, at the same time, we present a multi-tree search method for querying. When the number of tags is small, by replacing the actual ID with the temporary ID, it can greatly reduce the number of times that the reader reads and writes to tag's ID. Active tags send data to the reader by the way of modulation binary pulses. When applying this method to the uncertain ALOHA algorithms, the reader can determine the locations of the empty slots according to the position of the binary pulse, so it can avoid the decrease in efficiency which is caused by reading empty slots when reading slots. Theory and experiment show that this method can greatly improve the recognition efficiency of the system when applied to either the search tree or the ALOHA anti-collision algorithms.

  6. 78 FR 773 - Hartford Financial Services Group, Inc., Commercial/Actuarial/Information Delivery Services (IDS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-04

    .../ Information Delivery Services (IDS)/Corporate & Financial Reporting Group, Hartford, CT; Notice of Affirmative... (IDS)/Corporate & Financial Reporting group, Hartford, Connecticut (The Hartford-IDS Group). The... basis for the negative determination. The Hartford-IDS Group is engaged in activities related to...

  7. Fulminant atypical Cryptococcus neoformans pneumonia confirmed by PLEX-ID.

    PubMed

    Gariani, Karim; Rougemont, Mathieu; Renzi, Gesuele; Hibbs, Jonathan; Emonet, Stéphane; Schrenzel, Jacques

    2014-05-01

    Use of the PLEX-ID system can lead to a rapid molecular diagnosis in microbiology. To illustrate the clinical implications of this new diagnostic tool, we present the case of a 46-year-old patient admitted with severe respiratory failure and septic shock. Cryptococcal pneumonia was diagnosed by Fungi-Fluor™ staining of the bronchoalveolar lavage (BAL) and the patient tested positive for HIV. Unfortunately, he died 12h after admission despite intensive care support and treatment with broad-spectrum antibiotics, amphotericin B, and flucytosine. Retrospective use of the PLEX-ID on the BAL, bronchial aspirate, and blood yielded Cryptococcus neoformans in all fluids tested. Rapid molecular diagnosis with PLEX-ID, especially when performed on the blood of septic patients, may reduce the time to adequate treatment and limit the number of diagnostic procedures needed.

  8. Effect of ingredients on sensory profile of idli.

    PubMed

    Durgadevi, Manoharan; Shetty, Prathapkumar H

    2014-09-01

    Idli is a traditional fermented food and is consumed in India and Srilanka. The objective of the present study is to select the ingredients for optimum desirable product characteristics and to identify the optimum ratios of ingredients and fermentation time with respect to sensory attributes using Response Surface Methodology (RSM). The sensory attributes included were color, appearance, texture, taste and overall quality. Preliminary trials were conducted using five variants of rice and common black gram dhal before framing a model using Central Composite Rotatable Design (CCRD). From the study it was found that a desirable score of 0.7439 was obtained for sensory attributes of idli made with the ratio of 3: 1.475 for IR20 idli rice and ADT3 variety black gram (with husk removed after soaking) fermented for 10.2 h. Principal Component Analysis (PCA) helped to discriminate the samples and attributes within the data matrix, depending upon their inter relationships. PMID:25190832

  9. Installation, commissioning and performance of IDs installed at ALBA

    NASA Astrophysics Data System (ADS)

    Campmany, J.; Marcos, J.; Massana, V.; Becheri, F.; Gigante, J. V.; Colldelram, C.; Ribó, Ll

    2013-03-01

    The new synchrotron light source ALBA is currently starting regular operation. Up to 6 beamlines are using light produced by Insertion Devices. There are up to four types of IDs: 2 Apple-II undulators (EU62 and EU71) operating at low energies, one conventional wiggler (MPW80) operating in the range of 2 - 20 keV, two in-vacuum undulators (IVU21) operating in the range 5 - 30 keV and a superconducting wiggler (SCW30) operating in the range of (up to) 40 keV. The main IDs characteristics, their influence on the beam dynamics and a first characterization of their light will be presented.

  10. Phosphorylation Regulates Id2 Degradation and Mediates the Proliferation of Neural Precursor Cells

    PubMed Central

    Sullivan, Jaclyn M.; Havrda, Matthew C.; Kettenbach, Arminja N.; Paolella, Brenton R.; Zhang, Zhonghua; Gerber, Scott A.; Israel, Mark A.

    2016-01-01

    Inhibitor of DNA binding proteins (Id1-Id4) function to inhibit differentiation and promote proliferation of many different cell types. Among the Id family members, Id2 has been most extensively studied in the central nervous system (CNS). Id2 contributes to cultured neural precursor cell (NPC) proliferation as well as to the proliferation of CNS tumors such as glioblastoma that are likely to arise from NPC-like cells. We identified three phosphorylation sites near the N-terminus of Id2 in NPCs. To interrogate the importance of Id2 phosphorylation, Id2−/− NPCs were modified to express wild type (WT) Id2 or an Id2 mutant protein that could not be phosphorylated at the identified sites. We observed that NPCs expressing this mutant lacking phosphorylation near the N-terminus had higher steady-state levels of Id2 when compared to NPCs expressing WT Id2. This elevated level was the result of a longer half-life and reduced proteasome-mediated degradation. Moreover, NPCs expressing constitutively de-phosphorylated Id2 proliferated more rapidly than NPCs expressing WT Id2, a finding consistent with the well-characterized function of Id2 in driving proliferation. Observing that phosphorylation of Id2 modulates the degradation of this important cell-cycle regulator, we sought to identify a phosphatase that would stabilize Id2 enhancing its activity in NPCs and extended our analysis to include human glioblastoma-derived stem cells (GSCs). We found that expression of the phosphatase PP2A altered Id2 levels. Our findings suggest that inhibition of PP2A may be a novel strategy to regulate the proliferation of normal NPCs and malignant GSCs by decreasing Id2 levels. PMID:26756672

  11. 78 FR 65555 - Establishment of Class E Airspace; Salmon, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-01

    ... proposed rulemaking (NPRM) to establish controlled airspace at Salmon, ID (78 FR 45478). Interested parties...'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant... FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The incorporation by reference in 14 CFR...

  12. 77 FR 55688 - Amendment of Class E Airspace; Boise, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... E airspace designated as an extension at Boise Air Terminal, Boise, ID (77 FR 38552). Interested... FR 6026). Interested parties were invited to participate in this rulemaking effort by submitting... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation...

  13. 76 FR 70865 - Modification of Class E Airspace; Driggs, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ...), 40103, 40113, 40120; E. O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The... proposed rulemaking to modify controlled airspace at Driggs, ID (76 FR 56356). Interested parties were... a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26,...

  14. Student ID Cards: What You Should Know About Them

    ERIC Educational Resources Information Center

    Hoffman, Jonathan; McGuire, Agnes C.

    1973-01-01

    Discusses the usefulness of photo ID cards for school security purposes, library control, student activities, and bus transportation control. Examines ways in which card costs can be reduced and the pros and cons of producing the cards at the school or of letting the work out. Problems involving card abuse and student rights are also considered.…

  15. idRHa+ProMod - Rail Hardening Control System

    NASA Astrophysics Data System (ADS)

    Ferro, L.

    2016-03-01

    idRHa+ProMod is the process control system developed by Primetals Technologies to foresee the thermo-mechanical evolution and micro-structural composition of rail steels subjected to slack quenching into idRHa+ Rail Hardening equipments in a simulation environment. This tool can be used both off-line or in-line, giving the user the chance to test and study the best cooling strategies or letting the automatic control system free to adjust the proper cooling recipe. Optimization criteria have been tailored in order to determine the best cooling conditions according to the metallurgical requirements imposed by the main rail standards and also taking into account the elastoplastic bending phenomena occurring during all stages of the head hardening process. The computational core of idRHa+ProMod is a thermal finite element procedure coupled with special algorithms developed to work out the main thermo-physical properties of steel, to predict the non-isothermal austenite decomposition into all the relevant phases and subsequently to evaluate the amount of latent heat of transformation released, the compound thermal expansion coefficient and the amount of plastic deformation in the material. Air mist and air blades boundary conditions have been carefully investigated by means of pilot plant tests aimed to study the jet impingement on rail surfaces and the cooling efficiency at all working conditions. Heat transfer coefficients have been further checked and adjusted directly on field during commissioning. idRHa+ is a trademark of Primetals Technologies Italy Srl

  16. Retention Revisited: R=E, Id+E & In, Iv.

    ERIC Educational Resources Information Center

    Seidman, Alan

    1996-01-01

    Recent research on college student attrition is examined for trends, and it is suggested that the common perspective on retention and attrition is too narrow; it should be viewed from three perspectives: within-course retention; program retention; and institutional retention rate. Recommended for retention (R) is early (E) identification (Id) plus…

  17. 75 FR 61610 - Modification of Class E Airspace; Arco, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ... Federal Register a notice of proposed rulemaking to amend controlled airspace at Arco, ID (75 FR 41773... Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a... U.S.C. 106(g), 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec....

  18. 76 FR 22863 - Designation for the Lewiston, ID Area

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-25

    ... hours (7 CFR 1.27(c)). SUPPLEMENTARY INFORMATION: In the January 4, 2011, Federal Register (76 FR 317... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Grain Inspection, Packers and Stockyards Administration Designation for the Lewiston, ID Area...

  19. Imagery Measures of Ego, Id, Superego, and Identity: Validity Studies.

    ERIC Educational Resources Information Center

    Cartwright, D.; DeBruin, J.

    1988-01-01

    Five validity studies of the id, ego, superego, and identity scales of the Study of Imagery are reported, using undergraduate students. Multistage Bonferroni procedures are used to evaluate the significance of results. The scales are related to each other and to toughmindedness, self-control, and behavioral conflict. (TJH)

  20. Blueprints for Complex Learning: The 4C/ID-Model.

    ERIC Educational Resources Information Center

    van Merrienboer, Jeroen J. G.; Clark, Richard E.; de Croock, Marcel B. M.

    2002-01-01

    Describes the four-component instructional design system (4C/ID-model) developed for the design of training programs for complex skills. Discusses the structure of training blueprints for complex learning and associated instructional methods, focusing on learning tasks, supportive information, just-in-time information, and part-task practice.…

  1. Evolution of the ID Process at Sprint: A Practitioner's Perspective.

    ERIC Educational Resources Information Center

    Beard, Michael W.

    1999-01-01

    Provides instructional developers' (ID) insights and observations based on the experiences of a training and development organization, Sprint's University of Excellence (UE), as it evolved from a small group of 20-25 individuals in 1990 to a large business unit comprising almost 400 individuals in 1999. Discussion includes course development…

  2. An Application of Decision Tree Based on ID3

    NASA Astrophysics Data System (ADS)

    Xiaohu, Wang; Lele, Wang; Nianfeng, Li

    This article deals with the application of classical decision tree ID3 of the data mining in a certain site data. It constitutes a decision tree based on information gain and thus produces some useful purchasing behavior rules. It also proves that the decision tree has a wide applicable future in the sale field on site.

  3. Identification and expression profile of Id1 in bighead carp in response to microcystin-LR.

    PubMed

    Li, Huiying; Cai, Yan; Xie, Ping; Chen, Jun; Hao, Le; Li, Guangyu; Xiong, Qian

    2012-09-01

    Microcystin-LR (MCLR) is a widespread cyanotoxin produced in algal blooms, and has potent hepatotoxicity and tumor-promoting activity. We cloned the full-length cDNA of Id1 in bighead carp. The full-length Id1 cDNA was 954bp and contained a 387bp ORF. Bighead carp Id1 shared high identity with zebrafish Id1 amino acid sequence, and phylogenetic analysis showed that teleost Id1 evolved closely. Bighead carp Id1 constitutively expressed in all tested tissues in normal. When tested at two different time points post exposure and at 3 different MCLR doses, Id1 expression increased in a time-dependent pattern, and Id1 expression in brain was very sensitive to MCLR exposure. The present study will help us to understand more about the evolution of Id1 molecule and its role in the MCLR induced cell differentiation and cancer promoting in bighead carp. PMID:22683524

  4. Inhibitor of differentiation-4 (Id4) stimulates pigmentation in melanoma leading to histiocyte infiltration.

    PubMed

    DiVito, Kyle A; Trabosh, Valerie A; Chen, You-Shin; Simbulan-Rosenthal, Cynthia M; Rosenthal, Dean S

    2015-02-01

    TGF-β and the inhibitors of differentiation (Id) are linked. Smad7 and other TGF-β inhibitors can potently suppress melanomagenesis; however, little work examining Ids has been reported in melanoma, particularly for Id4. Here, we report that Id4, but not Id2 or Id3 expression, surprisingly, activated robust melanin production in xenografts of previously amelanotic (lacking pigment) 1205Lu/Smad7 (S7) cells. Fontana-Masson stain and de-novo expression of MART-1 and tyrosinase proteins confirmed melanin production. Additionally, pigment-laden CD163+ mouse histiocytes with areas of extensive necrosis were found throughout S7/Id4 tumors, but not in parental 1205Lu, S7/Id2 or S7Id3-derived tumors. Mechanistic investigation revealed increased nuclear M-microphthalmia-associated transcription factor (MITF) and MART-1 promoter activation following Id4 expression in 1205Lu and WM852 melanoma cells, suggesting broader implications for Id4 in melanin synthesis. In human tumors, melanin colocalized with Id4 expression establishing a correlation. Current chemotherapeutics for melanoma are only marginally effective. Immunotherapy provides the most promise, yet the role of innate immunity is poorly understood. Here, TGF-β suppression followed by Id4 expression results in extensive melanin synthesis and robust histiocyte recruitment following tumorigenesis, a novel role for Id4. Our results suggest that TGF-β suppression coupled with pigment overproduction triggers an innate immune response resulting in tumor necrosis.

  5. Comparison of Perinatal Risk Factors Associated with Autism Spectrum Disorder (ASD), Intellectual Disability (ID), and Co-occurring ASD and ID.

    PubMed

    Schieve, Laura A; Clayton, Heather B; Durkin, Maureen S; Wingate, Martha S; Drews-Botsch, Carolyn

    2015-08-01

    While studies report associations between perinatal outcomes and both autism spectrum disorder (ASD) and intellectual disability (ID), there has been little study of ASD with versus without co-occurring ID. We compared perinatal risk factors among 7547 children in the 2006-2010 Autism and Developmental Disability Monitoring Network classified as having ASD + ID, ASD only, and ID only. Children in all three groups had higher rates of preterm birth (PTB), low birth weight, small-for-gestational-age, and low Apgar score than expected based on the US birth cohort adjusted for key socio-demographic factors. Associations with most factors, especially PTB, were stronger for children with ID only than children with ASD + ID or ASD only. Associations were similar for children with ASD + ID and ASD only.

  6. Inhibitor of differentiation 4 (ID4) acts as an inhibitor of ID-1, -2 and -3 and promotes basic helix loop helix (bHLH) E47 DNA binding and transcriptional activity.

    PubMed

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-05-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID-interactions.

  7. Inhibitor of Differentiation 4 (ID4) Acts as an Inhibitor of ID-1, -2 and -3 and Promotes basic Helix Loop Helix (bHLH) E47 DNA Binding and Transcriptional Activity

    PubMed Central

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-01-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID- interactions. PMID:25778840

  8. Evaluation of red blood cell and platelet antigen genotyping platforms (ID CORE XT/ID HPA XT) in routine clinical practice

    PubMed Central

    Finning, Kirstin; Bhandari, Radhika; Sellers, Fiona; Revelli, Nicoletta; Villa, Maria Antonietta; Muñiz-Díaz, Eduardo; Nogués, Núria

    2016-01-01

    Background High-throughput genotyping platforms enable simultaneous analysis of multiple polymorphisms for blood group typing. BLOODchip® ID is a genotyping platform based on Luminex® xMAP technology for simultaneous determination of 37 red blood cell (RBC) antigens (ID CORE XT) and 18 human platelet antigens (HPA) (ID HPA XT) using the BIDS XT software. Materials and methods In this international multicentre study, the performance of ID CORE XT and ID HPA XT, using the centres’ current genotyping methods as the reference for comparison, and the usability and practicality of these systems, were evaluated under working laboratory conditions. DNA was extracted from whole blood in EDTA with Qiagen methodologies. Ninety-six previously phenotyped/genotyped samples were processed per assay: 87 testing samples plus five positive controls and four negative controls. Results Results were available for 519 samples: 258 with ID CORE XT and 261 with ID HPA XT. There were three “no calls” that were either caused by human error or resolved after repeating the test. Agreement between the tests and reference methods was 99.94% for ID CORE XT (9,540/9,546 antigens determined) and 100% for ID HPA XT (all 4,698 alleles determined). There were six discrepancies in antigen results in five RBC samples, four of which (in VS, N, S and Doa) could not be investigated due to lack of sufficient sample to perform additional tests and two of which (in S and C) were resolved in favour of ID CORE XT (100% accuracy). The total hands-on time was 28–41 minutes for a batch of 16 samples. Compared with the reference platforms, ID CORE XT and ID HPA XT were considered simpler to use and had shorter processing times. Discussion ID CORE XT and ID HPA XT genotyping platforms for RBC and platelet systems were accurate and user-friendly in working laboratory settings. PMID:26674823

  9. Subgroups associated with lower physical fitness in older adults with ID: results of the HA-ID study.

    PubMed

    Hilgenkamp, Thessa I M; van Wijck, Ruud; Evenhuis, Heleen M

    2014-02-01

    Although physical fitness is generally very low in older adults with intellectual disabilities (ID), levels may differ across subgroups. It is important to identify which subgroups need to be targeted specifically in physical activity and fitness interventions and reference values. Physical fitness was measured with box-and-block-test, response-time-test, Berg-balance-scale, walking speed, grip strength, 30s-chair-stand, 10 m incremental-shuttle-walking test and the extended modified-back-saver-sit-and-reach-test in a large sample of older adults with ID (n=1050), and subgroups associated with lower physical fitness levels were identified applying multivariate linear regression analyses. Both fixed personal characteristics such as being older, being female, having more severe ID and having Down syndrome and modifiable or preventable factors such as physical activity levels, mobility impairments and a need of more intensive care, are independently associated with lower levels of multiple physical fitness components. This first study identifies subgroups of older adults with ID which require adapted reference values, and subgroups that need to be specifically targeted in fitness promotion programs.

  10. County Staff or Area Staff?

    ERIC Educational Resources Information Center

    McIntyre, William J.

    1970-01-01

    McIntryre explores the questions of county-based extention and increased specialization in Indiana. He compares the multi-county with individual county systems using variables including clientele's reactions to the two systems. (NL)

  11. Identity crisis? The need for systematic gene IDs.

    PubMed

    Parsons, Marilyn; Myler, Peter J; Berriman, Matthew; Roos, David S; Stuart, Kenneth D

    2011-05-01

    Recent years have seen an explosion in the availability of protozoan pathogen genome sequences. Although data regarding the underlying genome sequence remain relatively stable after the initial draft, understanding of specific gene function is increasing rapidly. This dichotomy is reflected in the relative stability of systematic gene identifiers (SysIDs(*)) in genome sequence databases, as compared to evolving and/or conflicting gene and gene product names. GenBank/EMBL/DDBJ accession numbers are important, but most protozoan parasite researchers use organism-based databases such as EuPathDB or GeneDB as their immediate resource for gene-based information because they not only provide sequence information but also functional information and links to references. Reference to SysIDs therefore provides a valuable bridge to this repository of information.

  12. Tailoring a corporate compliance program for an IDS.

    PubMed

    Berkey, M F

    1999-01-01

    Integrated delivery systems (IDSs) face a particular challenge in implementing a corporate compliance program--that of ensuring the program addresses issues of compliance with payment rules and regulations consistently across every component of the health system. To successfully implement an effective compliance program, the various components of the IDS first must understand what such a program is intended to accomplish, and any internal resistance to developing the program must be overcome. The IDS then can undertake identifying and assessing areas of the health system at risk of being in noncompliance, designing and implementing the program, auditing claims processing and cost reporting throughout the health system to ensure systemwide compliance, and maintaining the program through ongoing monitoring and training. PMID:10339165

  13. Tailoring a corporate compliance program for an IDS.

    PubMed

    Berkey, M F

    1999-01-01

    Integrated delivery systems (IDSs) face a particular challenge in implementing a corporate compliance program--that of ensuring the program addresses issues of compliance with payment rules and regulations consistently across every component of the health system. To successfully implement an effective compliance program, the various components of the IDS first must understand what such a program is intended to accomplish, and any internal resistance to developing the program must be overcome. The IDS then can undertake identifying and assessing areas of the health system at risk of being in noncompliance, designing and implementing the program, auditing claims processing and cost reporting throughout the health system to ensure systemwide compliance, and maintaining the program through ongoing monitoring and training.

  14. Access Control for Mobile Assessment Systems Using ID.

    PubMed

    Nakayama, Masaharu; Ishii, Tadashi; Morino, Kazuma

    2015-01-01

    The assessment of shelters during disaster is critical to ensure the health of evacuees and prevent pandemic. In the Ishinomaki area, one of the areas most damaged by the Great East Japan Earthquake, the highly organized assessment helped to successfully manage a total of 328 shelters with a total of 46,480 evacuees. The input and analysis of vast amounts of data was tedious work for staff members. However, a web-based assessment system that utilized mobile devices was thought to decrease workload and standardize the evaluation form. The necessary access of information should be controlled in order to maintain individuals' privacy. We successfully developed an access control system using IDs. By utilizing a unique numerical ID, users can access the input form or assessment table. This avoids unnecessary queries to the server, resulting in a quick response and easy availability, even with poor internet connection. PMID:26262204

  15. Status and evolution of the ESRF beamline ID19

    SciTech Connect

    Weitkamp, Timm; Tafforeau, Paul; Boller, Elodie; Cloetens, Peter; Valade, Jean-Paul; Bernard, Pascal; Baruchel, Jose; Peyrin, Francoise; Helfen, Lukas

    2010-04-06

    The ESRF synchrotron beamline ID19, dedicated to full-field parallel-beam imaging techniques such as phase-contrast and absorption microtomography and X-ray topography, is one of the most versatile instruments of its kind. This paper presents key characteristics of ID19 in its present form, names examples for research and development performed on the beamline, and outlines the plans for an upgrade on the beamline in coming years, to adapt to the growing needs of the user community. The technical goals envisioned include an increase in available beam size and maximum photon energy, and a substantial increase in flux density for applications using beams of small and intermediate size.

  16. Status and evolution of the ESRF beamline ID19

    NASA Astrophysics Data System (ADS)

    Weitkamp, Timm; Tafforeau, Paul; Boller, Elodie; Cloetens, Peter; Valade, Jean-Paul; Bernard, Pascal; Peyrin, Françoise; Ludwig, Wolfgang; Helfen, Lukas; Baruchel, José

    2010-04-01

    The ESRF synchrotron beamline ID19, dedicated to full-field parallel-beam imaging techniques such as phase-contrast and absorption microtomography and X-ray topography, is one of the most versatile instruments of its kind. This paper presents key characteristics of ID19 in its present form, names examples for research and development performed on the beamline, and outlines the plans for an upgrade on the beamline in coming years, to adapt to the growing needs of the user community. The technical goals envisioned include an increase in available beam size and maximum photon energy, and a substantial increase in flux density for applications using beams of small and intermediate size.

  17. Developing an IDS-based disease-management program for the frail elderly.

    PubMed

    Baseman, S; Truxell, R

    2000-02-01

    IDSs that own a health plan or enter a full-risk-sharing contract with a payer are in an position to benefit from developing disease-management programs that reduce acute care admissions. Full-risk assumption allows an IDS to realize cost savings due to more cost-effective care management that offset revenue lost due to reduced acute care services. Crozer-Keystone Health System (CKHS) in Delaware County, Pennsylvania, implemented a disease-management program for frail elderly enrollees in its Medicare + Choice plan, MedCare Plus. Projected first-year savings were estimated at about $619,000 over 1,200 member months. First-year results showed a 36 percent reduction in acute care admissions, with savings of $242,749 over 613 member months. The difference between actual results and projected savings was attributed to social and other factors not accounted for in the cost-benefit analysis. Still, lost revenues from acute care admissions were more than offset by the savings realized.

  18. Suppression of Chondrogenesis by Id Helix-Loop-Helix Proteins in Murine Embryonic Orofacial Tissue

    PubMed Central

    Mukhopadhyay, Partha; Rezzoug, Francine; Webb, Cynthia L.; Pisano, M. Michele; Greene, Robert M.

    2009-01-01

    Inhibitors of differentiation (Id) proteins are helix-loop-helix (HLH) transcription factors lacking a DNA binding domain. Id proteins modulate cell proliferation, apoptosis, and differentiation in embryonic/fetal tissue. Perturbation of any of these processes in cells of the developing orofacial region results in orofacial anomalies. Chondrogenesis, a process integral to normal orofacial ontogenesis, is known to be modulated, in part, by Id proteins. In the present study, the mRNA and protein expression patterns of Id1, Id2, Id3 and Id4 were examined in developing murine orofacial tissue in vivo, as well as in murine embryonic maxillary mesenchymal cells in vitro. The functional role of Ids during chondrogenesis was also explored in vitro. Results reveal that cells derived from developing murine orofacial tissue: (1) express Id1, Id2, Id3 and Id4 mRNAs and proteins on each of gestational days 12-14, (2) express all four Id proteins in a developmentally regulated manner, (3) undergo chondrogenesis and express genes encoding various chondrogenic marker proteins (e.g. Runx2, Type X collagen, Sox9) when cultured under micromass conditions, and (4) can have their chondrogenic potential regulated via alteration of Id protein function through overexpression of a basic HLH factor. In summary, results from the current report reveal for the first time, the expression of all four Id proteins in cells derived from developing murine orofacial tissue, and demonstrate a functional role for the Ids in regulating the ability of these cells to undergo chondrogenesis. PMID:19349107

  19. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2012-04-01 2012-04-01 false Form ID, uniform...

  20. 17 CFR 259.602 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... OF 1935 Forms for Electronic Filing § 259.602 Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed by registrants, third party filers, or their agents, to whom the... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form ID, uniform...

  1. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form ID, uniform...

  2. 17 CFR 259.602 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... OF 1935 Forms for Electronic Filing § 259.602 Form ID, uniform application for access codes to file on EDGAR. Form ID must be filed by registrants, third party filers, or their agents, to whom the... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form ID, uniform...

  3. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form ID, uniform...

  4. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Form ID, uniform...

  5. 17 CFR 274.402 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Forms for Electronic Filing § 274.402 Form ID, uniform application for access codes to file on EDGAR... its Password. Editorial Note: For Federal Register citations affecting Form ID, see the List of CFR... 17 Commodity and Securities Exchanges 3 2013-04-01 2013-04-01 false Form ID, uniform...

  6. 78 FR 8596 - Hartford Financial Services Group, Inc., Commercial/Actuarial/ Information Delivery Services (IDS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... was published in the Federal Register on January 4, 2013 (78 FR 773). The Hartford-IDS Group is.../ Information Delivery Services (IDS)/Corporate & Financial Reporting Group, Hartford, CT; Notice of Negative... Services Group, Inc., Commercial/Actuarial/Information Delivery Services (IDS)/Corporate &...

  7. 77 FR 37696 - Agency Information Collection Activities: Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-22

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Importer ID Input... other Federal agencies to comment on an information collection requirement concerning the Importer ID... concerning the following information collection: Title: Importer ID Input Record. OMB Number: 1651-0064....

  8. 78 FR 67383 - Agency Information Collection Activities; Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-12

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities; Importer ID Input... other Federal agencies to comment on an information collection requirement concerning the Importer ID... concerning the following information collection: Title: Importer ID Input Record. OMB Number: 1651-0064....

  9. A Role for Id2 in Regulating Photic Entrainment of the Mammalian Circadian System

    PubMed Central

    Duffield, Giles E.; Watson, Nathan P.; Mantani, Akio; Peirson, Stuart N.; Robles-Murguia, Maricela; Loros, Jennifer J.; Israel, Mark A.; Dunlap, Jay C.

    2009-01-01

    Summary Inhibitor of DNA binding genes (Id1–Id4) encode helix-loop-helix (HLH) transcriptional repressors associated with development and tumorigenesis [1, 2], but little is known concerning the function(s) of these genes in normal adult animals. Id2 was identified in DNA microarray screens for rhythmically expressed genes [3–5], and further analysis revealed a circadian pattern of expression of all four Id genes in multiple tissues including the suprachiasmatic nucleus. To explore an in vivo function, we generated and characterized deletion mutations of Id2 and of Id4. Id2−/− mice exhibit abnormally rapid entrainment and an increase in the magnitude of the phase shift of the pacemaker. A significant proportion of mice also exhibit disrupted rhythms when maintained under constant darkness. Conversely, Id4−/− mice did not exhibit a noticeable circadian phenotype. In vitro studies using an mPer1 and an AVP promoter reporter revealed the potential for ID1, ID2, and ID3 proteins to interact with the canonical basic HLH clock proteins BMAL1 and CLOCK. These data suggest that the Id genes may be important for entrainment and operation of the mammalian circadian system, potentially acting through BMAL1 and CLOCK targets. PMID:19217292

  10. Picture This: How to Establish an Effective School ID Card Program

    ERIC Educational Resources Information Center

    Finkelstein, David

    2013-01-01

    Most school districts do not have an ID card policy that everyone knows and follows, yet. many school districts are implementing ID card programs to address concerns about safety, efficiency, and convenience. A well-thought-out ID card program leads to greater security and smoother operations throughout the school and should thus be a priority.…

  11. Id2 specifically alters regulation of the cell cycle by tumor suppressor proteins.

    PubMed Central

    Lasorella, A; Iavarone, A; Israel, M A

    1996-01-01

    Cells which are highly proliferative typically lack expression of differentiated, lineage-specific characteristics. Id2, a member of the helix-loop-helix (HLH) protein family known to inhibit cell differentiation, binds to the retinoblastoma protein (pRb) and abolishes its growth-suppressing activity. We found that Id2 but not Id1 or Id3 was able to bind in vitro not only pRb but also the related proteins p107 and p130. Also, an association between Id2 and p107 or p130 was observed in vivo in transiently transfected Saos-2 cells. In agreement with these results, expression of Id1 or Id3 did not affect the block of cell cycle progression mediated by pRb. Conversely, expression of Id2 specifically reversed the cell cycle arrest induced by each of the three members of the pRb family. Furthermore, the growth-suppressive activities of cyclin-dependent kinase inhibitors p16 and p21 were efficiently antagonized by high levels of Id2 but not by Id1 Id3. Consistent with the role of p16 as a selective inhibitor of pRb and pRb-related protein kinase activity, p16-imposed cell cycle arrest was completely abolished by Id2. Only a partial reversal of p21-induced growth suppression was observed, which correlated with the presence of a functional pRb. We also documented decreased levels of cyclin D1 protein and mRNA and the loss of cyclin D1-cdk4 complexes in cells constitutively expressing Id2. These data provide evidence for important Id2-mediated alterations in cell cycle components normally involved in the regulatory events of cell cycle progression, and they highlight a specific role for Id2 as an antagonist of multiple tumor suppressor proteins. PMID:8649364

  12. Structural organisation and chromosomal mapping of the human Id-3 gene.

    PubMed

    Deed, R W; Hirose, T; Mitchell, E L; Santibanez-Koref, M F; Norton, J D

    1994-12-30

    The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. We report here on the genomic organisation of the human Id3 (HLH 1R21/heir1) gene. Comparison with the two other mammalian Id genes, Id1 and Id2, reveals a highly conserved protein coding gene organisation consistent with evolution from a common, ancestral Id-like gene. In addition, by using a yeast artificial chromosome (YAC) clone of Id3, we have fine-scale mapped the gene to chromosome band 1p36.1 by fluorescence in situ hybridisation (FISH) and, using the same FISH technique, we have detected heterogeneity in tumour-associated 1p36 chromosome translocations.

  13. Impaired Thermogenesis and a Molecular Signature for Brown Adipose Tissue in Id2 Null Mice

    PubMed Central

    Zhou, Peng; Robles-Murguia, Maricela; Mathew, Deepa; Duffield, Giles E.

    2016-01-01

    Inhibitor of DNA binding 2 (ID2) is a helix-loop-helix transcriptional repressor rhythmically expressed in many adult tissues. Our previous studies have demonstrated that Id2 null mice have sex-specific elevated glucose uptake in brown adipose tissue (BAT). Here we further explored the role of Id2 in the regulation of core body temperature over the circadian cycle and the impact of Id2 deficiency on genes involved in insulin signaling and adipogenesis in BAT. We discovered a reduced core body temperature in Id2−/− mice. Moreover, in Id2−/− BAT, 30 genes including Irs1, PPARs, and PGC-1s were identified as differentially expressed in a sex-specific pattern. These data provide valuable insights into the impact of Id2 deficiency on energy homeostasis of mice in a sex-specific manner. PMID:27144179

  14. CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing

    PubMed Central

    Dehairs, Jonas; Talebi, Ali; Cherifi, Yacine; Swinnen, Johannes V.

    2016-01-01

    The advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform to directly identify indels and significantly speed up the characterization of clones. PMID:27363488

  15. Quadruplex Integrated DNA (QuID) Nanosensors for Monitoring Dopamine

    PubMed Central

    Morales, Jennifer M.; Skipwith, Christopher G.; Clark, Heather A.

    2015-01-01

    Dopamine is widely innervated throughout the brain and critical for many cognitive and motor functions. Imbalances or loss in dopamine transmission underlie various psychiatric disorders and degenerative diseases. Research involving cellular studies and disease states would benefit from a tool for measuring dopamine transmission. Here we show a Quadruplex Integrated DNA (QuID) nanosensor platform for selective and dynamic detection of dopamine. This nanosensor exploits DNA technology and enzyme recognition systems to optically image dopamine levels. The DNA quadruplex architecture is designed to be compatible in physically constrained environments (110 nm) with high flexibility, homogeneity, and a lower detection limit of 110 µM. PMID:26287196

  16. Psychometric properties of the Chinese version of Inventory for Depressive Symptomatology (IDS): Preliminary findings.

    PubMed

    Wu, Daxing; Yin, Huifang; Xu, Shujing; Carmody, Thomas; Morris, David W

    2010-09-01

    The aim of this study was to provide psychometric properties of the validity and reliability of the 30-item Inventory of Depressive Symptomatology as both a self-report (IDS-SR(30)) and as a clinician rating scale (IDS-C(30)) in a group of Chinese depressed outpatients. The Chinese version of IDS-C(30), IDS-SR(30), HRSD(17), BDI(13), SDS and ATQ were completed for 64 Chinese outpatients with major depressive disorder. The Cronbach's alphas for IDS-C(30) and IDS-SR(30) were 0.799 and 0.805, respectively. The coefficients test-retest reliability for the IDS-C(30) was 0.824 and for the IDSR-SR(30) was 0.718. The Pearson correlation coefficients between the IDS-C(30) and IDS-SR(30), and the other symptom measures (HRSD(17), BDI(13), and SDS) were high (from 0.738 to 0.882). The intra-class correlation coefficient showed the strength of agreement between the IDS-C(30) and IDS-SR(30), and the other symptom measures was found to be adequate (0.634-0.780). The Kappa coefficients of the severity of IDS-C(30) to HRSD(17), IDS-SR(30) were 0.50 and 0.40, respectively. This pilot study finding showed reasonable validity and reliability for the Chinese version of IDS(30) with highly acceptable psychometric properties. The inventory is suitable for assessing the severity of Chinese depressed patients, but this needs to be confirmed using a larger sample. Further studies should be done to demonstrate sensitivity of the scales to change of depression.

  17. Microbe-ID: an open source toolbox for microbial genotyping and species identification

    PubMed Central

    Tabima, Javier F.; Everhart, Sydney E.; Larsen, Meredith M.; Weisberg, Alexandra J.; Kamvar, Zhian N.; Tancos, Matthew A.; Smart, Christine D.; Chang, Jeff H.

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID. PMID:27602267

  18. Microbe-ID: an open source toolbox for microbial genotyping and species identification.

    PubMed

    Tabima, Javier F; Everhart, Sydney E; Larsen, Meredith M; Weisberg, Alexandra J; Kamvar, Zhian N; Tancos, Matthew A; Smart, Christine D; Chang, Jeff H; Grünwald, Niklaus J

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID.

  19. Microbe-ID: an open source toolbox for microbial genotyping and species identification.

    PubMed

    Tabima, Javier F; Everhart, Sydney E; Larsen, Meredith M; Weisberg, Alexandra J; Kamvar, Zhian N; Tancos, Matthew A; Smart, Christine D; Chang, Jeff H; Grünwald, Niklaus J

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID. PMID:27602267

  20. Microbe-ID: an open source toolbox for microbial genotyping and species identification

    PubMed Central

    Tabima, Javier F.; Everhart, Sydney E.; Larsen, Meredith M.; Weisberg, Alexandra J.; Kamvar, Zhian N.; Tancos, Matthew A.; Smart, Christine D.; Chang, Jeff H.

    2016-01-01

    Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (microbe-id.org) and provided a working implementation for the genus Phytophthora (phytophthora-id.org). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID.

  1. Comparison of Perinatal Risk Factors Associated with Autism Spectrum Disorder (ASD), Intellectual Disability (ID), and Co-Occurring ASD and ID

    ERIC Educational Resources Information Center

    Schieve, Laura A.; Clayton, Heather B.; Durkin, Maureen S.; Wingate, Martha S.; Drews-Botsch, Carolyn

    2015-01-01

    While studies report associations between perinatal outcomes and both autism spectrum disorder (ASD) and intellectual disability (ID), there has been little study of ASD with versus without co-occurring ID. We compared perinatal risk factors among 7547 children in the 2006-2010 Autism and Developmental Disability Monitoring Network classified as…

  2. ATF3 suppresses ESCC via downregulation of ID1

    PubMed Central

    Li, Jian; Yang, Zishan; Chen, Zhiuguo; Bao, Yonghua; Zhang, Huijuan; Fang, Xinhui; Yang, Wancai

    2016-01-01

    Esophageal cancer is one of the most prevalent forms of cancer and has a particularly high mortality rate due to early metastasis; however, the underlying mechanisms of its formation and progression remain unclear. The present study performed immunohistochemical analysis and observed that the expression of activating transcription factor 3 (ATF3) was reduced in esophageal squamous cell carcinoma (ESCC) in comparison with non-tumor adjacent tissues. By contrast, inhibitor of DNA binding 1 (ID1) was overexpressed in ESCC tissues, demonstrating an inverse correlation with ATF3 (P<0.01). In ESCC EC109 and KYSE450 cells lines, transfection with an ATF3-overexpression plasmid resulted in the inhibition of cell proliferation, motility and migration, which was associated with the induction of E-cadherin expression and inhibition of cyclin D1 and Twist. Notably, ATF3 exerted an inverse regulatory interaction with ID1. The results of the present study provide additional evidence of the tumor suppressive features of ATF3 and demonstrate a novel mechanism of ATF3-mediated inhibition of cancer metastasis in esophageal cancer. PMID:27602100

  3. Id or subego? Some theoretical questions for clinicians.

    PubMed

    Downey, T W

    1989-01-01

    This paper began with definitions and it will end with one; albeit a tentative one. Subego: The postulated realm of the mind derived from the infant-mother experience culminating in the development or maldevelopment of a partial sense of self as sensate, effective, and autonomous. A structure for driving and interpreting experience through largely unconscious visual imagery, a structure in a state of constant communication and overlap with the ego and superego. Reviewing what I have written leads to this afterword. Perhaps the present conceptualization of the id gives physiology and animal behavior a bad name. Perhaps we would advance structural theory more if we reserved the term "id" for the distorted state of wish fulfillment resulting from compromised parental malnurturing of the "good enough infant." Intrapsychic activities must to some degree be inimical. A better conceptual balance needs to be struck between their harmonious and antagonistic functioning. This condensed preliminary communication was intended to suggest some ways in which, in the spirit of Loewald, experience can give meaning to theory and theory can become a beacon for hardy clinicians seeking new avenues of exploration.

  4. DamID Analysis of Nuclear Organization in Caenorhabditis elegans.

    PubMed

    Gómez-Saldivar, Georgina; Meister, Peter; Askjaer, Peter

    2016-01-01

    The development of genomics and next generation sequencing platforms has dramatically improved our insight into chromatin structure and organization and its fine interplay with gene expression. The nuclear envelope has emerged as a key component in nuclear organization via extensive contacts between the genome and numerous proteins at the nuclear periphery. These contacts may have profound effects on gene expression as well as cell proliferation and differentiation. Indeed, their perturbations are associated with several human pathologies known as laminopathies or nuclear envelopathies. However, due to their dynamic behavior the contacts between nuclear envelope proteins and chromatin are challenging to identify, in particular in intact tissues. Here, we propose the DamID technique as an attractive method to globally characterize chromatin organization in the popular model organism Caenorhabditis elegans. DamID is based on the in vivo expression of a chromatin-associated protein of interest fused to the Escherichia coli DNA adenine methyltransferase, which produces unique identification tags at binding site in the genome. This marking is simple, highly specific and can be mapped by sensitive enzymatic and next generation sequencing approaches.

  5. ATF3 suppresses ESCC via downregulation of ID1

    PubMed Central

    Li, Jian; Yang, Zishan; Chen, Zhiuguo; Bao, Yonghua; Zhang, Huijuan; Fang, Xinhui; Yang, Wancai

    2016-01-01

    Esophageal cancer is one of the most prevalent forms of cancer and has a particularly high mortality rate due to early metastasis; however, the underlying mechanisms of its formation and progression remain unclear. The present study performed immunohistochemical analysis and observed that the expression of activating transcription factor 3 (ATF3) was reduced in esophageal squamous cell carcinoma (ESCC) in comparison with non-tumor adjacent tissues. By contrast, inhibitor of DNA binding 1 (ID1) was overexpressed in ESCC tissues, demonstrating an inverse correlation with ATF3 (P<0.01). In ESCC EC109 and KYSE450 cells lines, transfection with an ATF3-overexpression plasmid resulted in the inhibition of cell proliferation, motility and migration, which was associated with the induction of E-cadherin expression and inhibition of cyclin D1 and Twist. Notably, ATF3 exerted an inverse regulatory interaction with ID1. The results of the present study provide additional evidence of the tumor suppressive features of ATF3 and demonstrate a novel mechanism of ATF3-mediated inhibition of cancer metastasis in esophageal cancer.

  6. ID4 promotes AR expression and blocks tumorigenicity of PC3 prostate cancer cells.

    PubMed

    Komaragiri, Shravan Kumar; Bostanthirige, Dhanushka H; Morton, Derrick J; Patel, Divya; Joshi, Jugal; Upadhyay, Sunil; Chaudhary, Jaideep

    2016-09-01

    Deregulation of tumor suppressor genes is associated with tumorigenesis and the development of cancer. In prostate cancer, ID4 is epigenetically silenced and acts as a tumor suppressor. In normal prostate epithelial cells, ID4 collaborates with androgen receptor (AR) and p53 to exert its tumor suppressor activity. Previous studies have shown that ID4 promotes tumor suppressive function of AR whereas loss of ID4 results in tumor promoter activity of AR. Previous study from our lab showed that ectopic ID4 expression in DU145 attenuates proliferation and promotes AR expression suggesting that ID4 dependent AR activity is tumor suppressive. In this study, we examined the effect of ectopic expression of ID4 on highly malignant prostate cancer cell, PC3. Here we show that stable overexpression of ID4 in PC3 cells leads to increased apoptosis and decreased cell proliferation and migration. In addition, in vivo studies showed a decrease in tumor size and volume of ID4 overexpressing PC3 cells, in nude mice. At the molecular level, these changes were associated with increased androgen receptor (AR), p21, and AR dependent FKBP51 expression. At the mechanistic level, ID4 may regulate the expression or function of AR through specific but yet unknown AR co-regulators that may determine the final outcome of AR function.

  7. 77 FR 72968 - Revisions to the California State Implementation Plan, for Imperial County, Placer County and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-07

    ... County and Ventura County Air Pollution Control Districts AGENCY: Environmental Protection Agency (EPA... Imperial County Air Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD) and Ventura County Air Pollution Control District (PCAPCD) portions of the California...

  8. 6 CFR 37.29 - Prohibition against holding more than one REAL ID card or more than one driver's license.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ID card or more than one driver's license. 37.29 Section 37.29 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum... REAL ID card or more than one driver's license. (a) An individual may hold only one REAL ID card....

  9. 6 CFR 37.29 - Prohibition against holding more than one REAL ID card or more than one driver's license.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ID card or more than one driver's license. 37.29 Section 37.29 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum... REAL ID card or more than one driver's license. (a) An individual may hold only one REAL ID card....

  10. Identifier (ID) elements are not preferentially located to brain-specific genes: high ID element representation in other tissue-specific- and housekeeping genes of the rat.

    PubMed

    Goldman, Andrés; Capoano, Carlos A; González-López, Evangelina; Geisinger, Adriana

    2014-01-01

    BC1 is a short non-coding RNA from rodents, which is transcribed by RNA pol III. Its RNA is highly abundant in the brain, where it exerts a post-transcriptional regulatory role in dendrites. Upon transcription, retroposition and insertion, BC1 gives rise to a subclass of short interspersed repetitive sequences (SINEs) named identifier (ID) elements. IDs can become integrated inside non-coding regions of RNA pol II transcription units, and - although challenged by a couple of reports - their preferential location to brain-specific genes has been long proposed. Furthermore, an additional, cis-regulatory role in the control of brain-specific pol II-directed transcripts has been suggested for these sequences. In this work we used Northern blot and in silico analyses to examine IDs' location among pol II transcription units in different tissues, and in housekeeping genes. ID sequences appeared distributed in a similar fashion within tissue-specific hnRNA populations of the brain, testis and liver, and within housekeeping primary transcripts as well. Moreover, when the lengths of the unprocessed transcripts were considered, ID representation was higher in housekeeping ones. On the other hand, ID elements appeared similarly distributed among the different gene regions, with the obvious exclusion of those sequences where strict constraints for proper gene expression exist. Altogether, the widespread distribution of ID elements in all the analyzed genes - including housekeeping - and in all gene regions, suggests a random location, raising questions about the specific cis-regulatory role of those sequences.

  11. An improved smaller biotin ligase for BioID proximity labeling

    PubMed Central

    Kim, Dae In; Jensen, Samuel C.; Noble, Kyle A.; KC, Birendra; Roux, Kenneth H.; Motamedchaboki, Khatereh; Roux, Kyle J.

    2016-01-01

    The BioID method uses a promiscuous biotin ligase to detect protein–protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein–protein associations. We also demonstrate that the biotinylation range of BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adjustment of the biotin-labeling radius. PMID:26912792

  12. ID-Check: Online Concealed Information Test Reveals True Identity.

    PubMed

    Verschuere, Bruno; Kleinberg, Bennett

    2016-01-01

    The Internet has already changed people's lives considerably and is likely to drastically change forensic research. We developed a web-based test to reveal concealed autobiographical information. Initial studies identified a number of conditions that affect diagnostic efficiency. By combining these moderators, this study investigated the full potential of the online ID-check. Participants (n = 101) tried to hide their identity and claimed a false identity in a reaction time-based Concealed Information Test. Half of the participants were presented with personal details (e.g., first name, last name, birthday), whereas the others only saw irrelevant details. Results showed that participants' true identity could be detected with high accuracy (AUC = 0.98; overall accuracy: 86-94%). Online memory detection can reliably and validly detect whether someone is hiding their true identity. This suggests that online memory detection might become a valuable tool for forensic applications. PMID:26390033

  13. Image processing by Intensity-Dependent Spread (IDS)

    NASA Technical Reports Server (NTRS)

    Cornsweet, Tom N.

    1989-01-01

    As retinal illuminance is lowered, the human visual system integrates the effects of photon absorptions over larger areas and longer times. A theory of the process that might underlie these changes is called Intensity-Dependent Spread (IDS). Each input point gives rise to a pattern of excitation that spreads to a region of the total excitation it sees. The unique aspect of the theory is the assumption that, although the amplitude of the excitation pattern at its center increases with input illuminance, its width decreases in such a way that its volume remains constant. Application of this theory to image processing reveals that it displays a number of unexpected and potentially useful properties. Among them are edge enhancement and independence from scene illumination.

  14. ID-Check: Online Concealed Information Test Reveals True Identity.

    PubMed

    Verschuere, Bruno; Kleinberg, Bennett

    2016-01-01

    The Internet has already changed people's lives considerably and is likely to drastically change forensic research. We developed a web-based test to reveal concealed autobiographical information. Initial studies identified a number of conditions that affect diagnostic efficiency. By combining these moderators, this study investigated the full potential of the online ID-check. Participants (n = 101) tried to hide their identity and claimed a false identity in a reaction time-based Concealed Information Test. Half of the participants were presented with personal details (e.g., first name, last name, birthday), whereas the others only saw irrelevant details. Results showed that participants' true identity could be detected with high accuracy (AUC = 0.98; overall accuracy: 86-94%). Online memory detection can reliably and validly detect whether someone is hiding their true identity. This suggests that online memory detection might become a valuable tool for forensic applications.

  15. 76 FR 55788 - Amendments To Include New Applicant Types on Form ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-09

    ... processing of the form. Form ID is the application for access codes to file on the Commission's Electronic..., 2011), 76 FR 10948 (February 28, 2011) (File No. S7-06-11). \\32\\ Id. \\33\\ See 15 U.S.C. 78c(a)(77) (as... COMMISSION 17 CFR Parts 239, 249, 269 and 274 Amendments To Include New Applicant Types on Form ID...

  16. Id-1: Regulator of EGFR and VEGF and potential target for colorectal cancer therapy

    PubMed Central

    Meteoglu, Ibrahim; Meydan, Nezih; Erkus, Muhan

    2008-01-01

    Background The helix-loop-helix transcription factor Id-1 (an inhibitor of differentiation and DNA binding) plays a role in development and progression of many tumours. Id-1 is known to exert its effects on the epidermal growth factor receptor (EGFR) and the vascular endothelial growth factor (VEGF). The aim of this study was to reveal whether there was a relationship between Id-1 and EGFR and VEGF in colorectal carcinoma. Methods Tumour and non-tumour tissue specimens from 46 cases of colorectal carcinoma were exposed to immunohistochemical staining for Id-1, EGFR and VEGF. The relationship between the degree of staining and tumour grade, tumour stage and all tumour markers was investigated. Results Tumour cells showed positive staining for Id-1 in 43 cases (93.5%), for EGFR in 41 cases (89%) and for VEGF in 42 cases (91%). There was a significant relation between the tumour grade and the degree of staining for Id-1, EGFR and VEGF. The relation between the tumour stage and the degree of staining for Id-1, EGFR and VEGF was also significant. There was a significant relation between Id-1 expression and EGFR and VEGF expressions. Non-tumoural tissue specimens were not stained with Id-1 and EGFR antibodies in any of the cases, but stained with VEGF antibody in 3 cases. Conclusion This study revealed that Id-1, EGFR and VEGF took part in development and progression of colorectal carcinomas and that Id-1 was associated with regulations of EGFR and VEGF. The results of this study support the idea that not only EGFR and VEGF but also Id-1 could be new targets in cancer treatment. PMID:19014499

  17. The regulation and function of the Id proteins in lymphocyte development.

    PubMed

    Rivera, R; Murre, C

    2001-12-20

    Helix-loop-helix (HLH) proteins are essential factors for lymphocyte development and function. One class of HLH proteins, the E-proteins, regulate many aspects of lymphocyte maturation, survival, proliferation, and differentiation. E-proteins are negatively regulated by another class of HLH proteins known as the Id proteins. The Id proteins function as dominant negative inhibitors of E-proteins by inhibiting their ability to bind DNA. Here we discuss the function and regulation of the Id proteins in lymphocyte development.

  18. The Implementation of C-ID, R2D2 Model on Learning Reading Comprehension

    ERIC Educational Resources Information Center

    Rayanto, Yudi Hari; Rusmawan, Putu Ngurah

    2016-01-01

    The purposes of this research are to find out, (1) whether C-ID, R2D2 model is effective to be implemented on learning Reading comprehension, (2) college students' activity during the implementation of C-ID, R2D2 model on learning Reading comprehension, and 3) college students' learning achievement during the implementation of C-ID, R2D2 model on…

  19. Underground Storage Tank Integrated Demonstration (UST-ID). Technology summary

    SciTech Connect

    Not Available

    1994-02-01

    The DOE complex currently has 332 underground storage tanks (USTs) that have been used to process and store radioactive and chemical mixed waste generated from weapon materials production. Very little of the over 100 million gallons of high-level and low-level radioactive liquid waste has been treated and disposed of in final form. Two waste storage tank design types are prevalent across the DOE complex: single-shell wall and double-shell wall designs. They are made of stainless steel, concrete, and concrete with carbon steel liners, and their capacities vary from 5000 gallons (19 m{sup 3}) to 10{sup 6} gallons (3785 m{sup 3}). The tanks have an overburden layer of soil ranging from a few feet to tens of feet. Responding to the need for remediation of tank waste, driven by Federal Facility Compliance Agreements (FFCAs) at all participating sites, the Underground Storage Tank Integrated Demonstration (UST-ID) Program was created by the US DOE Office of Technology Development in February 1991. Its mission is to focus the development, testing, and evaluation of remediation technologies within a system architecture to characterize, retrieve, treat to concentrate, and dispose of radioactive waste stored in USTs at DOE facilities. The ultimate goal is to provide safe and cost-effective solutions that are acceptable to the public and the regulators. The UST-ID has focused on five DOE locations: the Hanford Site, which is the host site, in Richland, Washington; the Fernald Site in Fernald, Ohio; the Idaho National Engineering Laboratory near Idaho Falls, Idaho; the Oak Ridge Reservation in Oak Ridge, Tennessee, and the Savannah River Site in Savannah River, South Carolina.

  20. DeID - a data sharing tool for neuroimaging studies.

    PubMed

    Song, Xuebo; Wang, James; Wang, Anlin; Meng, Qingping; Prescott, Christian; Tsu, Loretta; Eckert, Mark A

    2015-01-01

    Funding institutions and researchers increasingly expect that data will be shared to increase scientific integrity and provide other scientists with the opportunity to use the data with novel methods that may advance understanding in a particular field of study. In practice, sharing human subject data can be complicated because data must be de-identified prior to sharing. Moreover, integrating varied data types collected in a study can be challenging and time consuming. For example, sharing data from structural imaging studies of a complex disorder requires the integration of imaging, demographic and/or behavioral data in a way that no subject identifiers are included in the de-identified dataset and with new subject labels or identification values that cannot be tracked back to the original ones. We have developed a Java program that users can use to remove identifying information in neuroimaging datasets, while still maintaining the association among different data types from the same subject for further studies. This software provides a series of user interaction wizards to allow users to select data variables to be de-identified, implements functions for auditing and validation of de-identified data, and enables the user to share the de-identified data in a single compressed package through various communication protocols, such as FTPS and SFTP. DeID runs with Windows, Linux, and Mac operating systems and its open architecture allows it to be easily adapted to support a broader array of data types, with the goal of facilitating data sharing. DeID can be obtained at http://www.nitrc.org/projects/deid. PMID:26441500

  1. Snohomish County Biodiesel Project

    SciTech Connect

    Chang, Terrill; Carveth, Deanna

    2010-02-01

    Snohomish County in western Washington State began converting its vehicle fleet to use a blend of biodiesel and petroleum diesel in 2005. As prices for biodiesel rose due to increased demand for this cleaner-burning fuel, Snohomish County looked to its farmers to grow this fuel locally. Suitable seed crops that can be crushed to extract oil for use as biodiesel feedstock include canola, mustard, and camelina. The residue, or mash, has high value as an animal feed. County farmers began with 52 acres of canola and mustard crops in 2006, increasing to 250 acres and 356 tons in 2008. In 2009, this number decreased to about 150 acres and 300 tons due to increased price for mustard seed.

  2. Idaho National Laboratory Supervisory Control and Data Acquisition Intrusion Detection System (SCADA IDS)

    SciTech Connect

    Jared Verba; Michael Milvich

    2008-05-01

    Current Intrusion Detection System (IDS) technology is not suited to be widely deployed inside a Supervisory, Control and Data Acquisition (SCADA) environment. Anomaly- and signature-based IDS technologies have developed methods to cover information technology-based networks activity and protocols effectively. However, these IDS technologies do not include the fine protocol granularity required to ensure network security inside an environment with weak protocols lacking authentication and encryption. By implementing a more specific and more intelligent packet inspection mechanism, tailored traffic flow analysis, and unique packet tampering detection, IDS technology developed specifically for SCADA environments can be deployed with confidence in detecting malicious activity.

  3. Id expression in amphioxus and lamprey highlights the role of gene cooption during neural crest evolution

    NASA Technical Reports Server (NTRS)

    Meulemans, Daniel; McCauley, David; Bronner-Fraser, Marianne

    2003-01-01

    Neural crest cells are unique to vertebrates and generate many of the adult structures that differentiate them from their closest invertebrate relatives, the cephalochordates. Id genes are robust markers of neural crest cells at all stages of development. We compared Id gene expression in amphioxus and lamprey to ask if cephalochordates deploy Id genes at the neural plate border and dorsal neural tube in a manner similar to vertebrates. Furthermore, we examined whether Id expression in these cells is a basal vertebrate trait or a derived feature of gnathostomes. We found that while expression of Id genes in the mesoderm and endoderm is conserved between amphioxus and vertebrates, expression in the lateral neural plate border and dorsal neural tube is a vertebrate novelty. Furthermore, expression of lamprey Id implies that recruitment of Id genes to these cells occurred very early in the vertebrate lineage. Based on expression in amphioxus we postulate that Id cooption conferred sensory cell progenitor-like properties upon the lateral neurectoderm, and pharyngeal mesoderm-like properties upon cranial neural crest. Amphioxus Id expression is also consistent with homology between the anterior neurectoderm of amphioxus and the presumptive placodal ectoderm of vertebrates. These observations support the idea that neural crest evolution was driven in large part by cooption of multipurpose transcriptional regulators from other tissues and cell types.

  4. Targeting Id protein interactions by an engineered HLH domain induces human neuroblastoma cell differentiation.

    PubMed

    Ciarapica, R; Annibali, D; Raimondi, L; Savino, M; Nasi, S; Rota, R

    2009-04-30

    Inhibitor of DNA-binding (Id) proteins prevent cell differentiation, promote growth and sustain tumour development. They do so by binding to E proteins and other transcription factors through the helix-loop-helix (HLH) domain, and inhibiting transcription. This makes HLH-mediated Id protein interactions an appealing therapeutic target. We have used the dominant interfering HLH dimerization mutant 13I to model the impact of Id inhibition in two human neuroblastoma cell lines: LA-N-5, similar to immature neuroblasts, and SH-EP, resembling more immature precursor cells. We have validated 13I as an Id inhibitor by showing that it selectively binds to Ids, impairs complex formation with RB, and relieves repression of E protein-activated transcription. Id inactivation by 13I enhances LA-N-5 neural features and causes SH-EP cells to acquire neuronal morphology, express neuronal proteins such as N-CAM and NF-160, proliferate more slowly, and become responsive to retinoic acid. Concomitantly, 13I augments the cell-cycle inhibitor p27(Kip1) and reduces the angiogenic factor vascular endothelial growth factor. These effects are Id specific, being counteracted by Id overexpression. Furthermore, 13I strongly impairs tumorigenic properties in agar colony formation and cell invasion assays. Targeting Id dimerization may therefore be effective for triggering differentiation and restraining neuroblastoma cell tumorigenicity.

  5. Automatic spent fuel ID-number reader (II) image-processing technique

    SciTech Connect

    Kubo, K.; Mizuguchi, H.; Miyazawa, T. )

    1991-01-01

    This paper reports on an image-processing technique for automatic spent fuel ID-number reader system. As a result, efficiency and reliability of the reader system is improved. To obtain reliable ID-number identification-capability, there are many items to be considered. Some of the important ones are appropriate lighting, high speed processors and suitable algorithm. After developing the technique on the items, the applicability of the image-processing technique for the reader system is verified. ID-numbers are accurately identified, and the time required for one ID-number reading is obtained in about 2 seconds. The result is one tenth of the conventional visual identification.

  6. A comprehensive protein-centric ID mapping service for molecular data integration

    PubMed Central

    Huang, Hongzhan; Suzek, Baris E.; Mazumder, Raja; Zhang, Jian; Chen, Yongxing; Wu, Cathy H.

    2011-01-01

    Motivation: Identifier (ID) mapping establishes links between various biological databases and is an essential first step for molecular data integration and functional annotation. ID mapping allows diverse molecular data on genes and proteins to be combined and mapped to functional pathways and ontologies. We have developed comprehensive protein-centric ID mapping services providing mappings for 90 IDs derived from databases on genes, proteins, pathways, diseases, structures, protein families, protein interaction, literature, ontologies, etc. The services are widely used and have been regularly updated since 2006. Availability: www.uniprot.org/mappingandproteininformation-resource.org/pirwww/search/idmapping.shtml Contact: huang@dbi.udel.edu PMID:21478197

  7. Fake ID ownership and heavy drinking in underage college students: prospective findings.

    PubMed

    Martinez, Julia A; Rutledge, Patricia C; Sher, Kenneth J

    2007-06-01

    The authors examined the ownership of false identification (fake ID) for the purpose of obtaining alcohol and the relation of fake ID ownership to heavy drinking in a longitudinal sample of college students under 21 years of age. A sample of 3,720 undergraduates was assessed the summer prior to college entrance and during the 4 semesters comprising freshman and sophomore years. Regression analyses were used to estimate bidirectional relations between consumption and fake ID ownership. Sex, Greek membership, and prior drinking were controlled. Results showed that fake ID ownership increased over time (12.5% pre-college to 32.2% fourth semester) and that Greek members were more likely than others to own fake IDs. Fake ID ownership predicted concurrent and next-semester heavy drinking with increasing strength over time. Also, the acquisition (onset) of fake ID ownership at each time point was predicted by previous-semester consumption. When traditional, robust risk factors of consumption are controlled, fake ID ownership meaningfully relates to heavy drinking in college. It thus presents a significant public health problem, addressable through training for alcohol servers and retailers, punitive measures toward fake ID owners, and other possible interventions.

  8. Ebf1-mediated down-regulation of Id2 and Id3 is essential for specification of the B cell lineage

    PubMed Central

    Thal, Melissa A.; Carvalho, Thiago L.; He, Ti; Kim, Hyung-Gyoon; Gao, Hua; Hagman, James; Klug, Christopher A.

    2009-01-01

    Gene knockout experiments in mice have suggested a hierarchical model of early B cell commitment wherein E2A proteins (E47 and E12) activate early B cell factor (Ebf1), which in turn activates expression of the B cell commitment factor, Pax5. In IL-7 receptor alpha (IL-7Rα) knockout mice, B cell development is blocked before B-lineage commitment at the prepro-B cell stage in adult animals. In IL-7Rα−/− prepro-B cells, E47 is expressed and yet is insufficient to transcriptionally activate the putative downstream target gene, Ebf1. In this study, we show that further increases of E47 expression in IL-7Rα−/− prepro-B cells fails to activate Ebf1, but rather leads to a dramatic induction of the E2A inhibitory factors, Id2 and Id3. In contrast, enforced expression of Ebf1 in IL-7Rα−/− bone marrow potently down-regulates Id2 and Id3 mRNA expression and restores B cell differentiation in vivo. Down-regulation of both Id2 and Id3 during B cell specification is essential in that overexpression of either Id2 or Id3 in wild-type bone marrow blocks B cell specification at the prepro-B cell stage. Collectively, these studies suggest a model where Ebf1 induction specifies the B cell fate by dramatically increasing activity of E47 at the posttranslational level. PMID:19122139

  9. High-speed readout method of ID information on a large amount of electronic tags

    NASA Astrophysics Data System (ADS)

    Nagate, Wataru; Sasabe, Masahiro; Nakano, Hirotaka

    2006-10-01

    An electronic tag such as RFID is expected to create new services that cannot be achieved by the traditional bar code. Specifically, in a distribution system, simultaneous readout method of a large amount of electronic tags embedded in products is required to reduce costs and time. In this paper, we propose novel methods, called Response Probability Control (RPC), to accomplish this requirement. In RPC, a reader firstly sends an ID request to electronic tags in its access area. It succeeds reading information on a tag only if other tags do not respond. To improve the readout efficiency, the reader appropriately controls the response probability in accordance with the number of tags. However, this approach cannot entirely avoid a collision of multiple responses. When a collision occurs, ID information is lost. To reduce the amount of lost data, we divide the ID registration process into two steps. The reader first gathers the former part of the original ID, called temporal ID, according to the above method. After obtaining the temporal ID, it sequentially collects the latter part of ID, called remaining ID, based on the temporal ID. Note that we determine the number of bits of a temporal ID in accordance with the number of tags in the access area so that each tag can be distinguishable. Through simulation experiments, we evaluate RPC in terms of the readout efficiency. Simulation results show that RPC can accomplish the readout efficiency 1.17 times higher than the traditional method where there are a thousand of electronic tags whose IDs are 128 bits.

  10. Id2 and E Proteins Orchestrate the Initiation and Maintenance of MLL-Rearranged Acute Myeloid Leukemia.

    PubMed

    Ghisi, Margherita; Kats, Lev; Masson, Frederick; Li, Jason; Kratina, Tobias; Vidacs, Eva; Gilan, Omer; Doyle, Maria A; Newbold, Andrea; Bolden, Jessica E; Fairfax, Kirsten A; de Graaf, Carolyn A; Firth, Matthew; Zuber, Johannes; Dickins, Ross A; Corcoran, Lynn M; Dawson, Mark A; Belz, Gabrielle T; Johnstone, Ricky W

    2016-07-11

    E proteins and their antagonists, the Id proteins, are transcriptional regulators important for normal hematopoiesis. We found that Id2 acts as a key regulator of leukemia stem cell (LSC) potential in MLL-rearranged acute myeloid leukemia (AML). Low endogenous Id2 expression is associated with LSC enrichment while Id2 overexpression impairs MLL-AF9-leukemia initiation and growth. Importantly, MLL-AF9 itself controls the E-protein pathway by suppressing Id2 while directly activating E2-2 expression, and E2-2 depletion phenocopies Id2 overexpression in MLL-AF9-AML cells. Remarkably, Id2 tumor-suppressive function is conserved in t(8;21) AML. Low expression of Id2 and its associated gene signature are associated with poor prognosis in MLL-rearranged and t(8;21) AML patients, identifying the Id2/E-protein axis as a promising new therapeutic target in AML. PMID:27374225

  11. FHL2 interacts with and acts as a functional repressor of Id2 in human neuroblastoma cells

    PubMed Central

    Han, Weidong; Wu, Zhiqiang; Zhao, Yali; Meng, Yuanguang; Si, Yiling; Yang, Jie; Fu, Xiaobing; Yu, Li

    2009-01-01

    Inhibitor of differentiation 2 (Id2) is a natural inhibitor of the basic helix–loop–helix transcription factors. Although Id2 is well known to prevent differentiation and promote cell-cycle progression and tumorigenesis, the molecular events that regulate Id2 activity remain to be investigated. Here, we identified that Four-and-a-half LIM-only protein 2 (FHL2) is a novel functional repressor of Id2. Moreover, we demonstrated that FHL2 can directly interact with all members of the Id family (Id1–4) via an N-terminal loop–helix structure conserved in Id proteins. FHL2 antagonizes the inhibitory effect of Id proteins on basic helix–loop–helix protein E47-mediated transcription, which was abrogated by the deletion mutation of Ids that disrupted their interaction with FHL2. We also showed a competitive nature between FHL2 and E47 for binding Id2, whereby FHL2 prevents the formation of the Id2–E47 heterodimer, thus releasing E47 to DNA and restoring its transcriptional activity. FHL2 expression was remarkably up-regulated during retinoic acid-induced differentiation of neuroblastoma cells, during which the expression of Id2 was opposite to that. Ectopic FHL2 expression in neuroblastoma cells markedly reduces the transcriptional and cell-cycle promoting functions of Id2. Altogether, these results indicate that FHL2 is an important repressor of the oncogenic activity of Id2 in neuroblastoma cells. PMID:19417068

  12. 77 FR 59639 - Bear Lake National Wildlife Refuge, Bear Lake County, ID and Oxford Slough Waterfowl Production...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ... environmental education and interpretation. We will review and update the CCP at least every 15 years in... and environmental education programs to local schools and community groups. Alternative 3 (Preferred... interpretive and environmental education programs to local schools and community groups. Public Availability...

  13. 78 FR 33433 - Bear Lake National Wildlife Refuge, Bear Lake County, ID, and Oxford Slough Waterfowl Production...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-04

    ... Federal Register (75 FR 35829; June 23, 2010). We released the draft CCP/EA to the public, announcing and requesting comments in a notice of availability in the Federal Register (77 FR 59639; September 28, 2012... public comments on the draft CCP/EA for 30 days, from September 28 to October 29, 2012 (77 FR 59639)....

  14. 75 FR 65649 - Notice of Realty Action: Segregation To Consider Proposed Sale of Public Lands in Blaine County, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-26

    ... Bureau of Land Management Notice of Realty Action: Segregation To Consider Proposed Sale of Public Lands... laws, including the mining laws, except the sale provisions of FLPMA. The BLM is no longer accepting..., publication in the Federal Register of a termination of the segregation, or October 26, 2012 unless...

  15. 76 FR 66034 - Proposed Foreign-Trade Zone-Ada and Canyon Counties, ID, Under Alternative Site Framework...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ... (CBP) port of entry, under the alternative site framework (ASF) adopted by the Board (74 FR 1170-1173, 1/12/09 (correction 74 FR 3987, 1/22/09); 75 FR 71069-71070, 11/22/10). The ASF is an option for... of entry. FTZ 192 in Meridian, Idaho was established on February 17, 1993 (Board Order 629, 58...

  16. Four hour identification of Enterobacteriaceae with the API Rapid 20E and Micro-ID systems.

    PubMed Central

    Holmes, B; Humphry, P S

    1985-01-01

    One hundred strains of Enterobacteriaceae were examined in parallel with the API Rapid 20E and Micro-ID commercial four hour identification systems. With the API Rapid 20E system 78% of the strains were correctly identified, 15% were not identified, and 7% were misidentified. The respective figures with the Micro-ID system were 74%, 11%, and 15%. PMID:3902898

  17. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... identification cards. 37.23 Section 37.23 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  18. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... identification cards. 37.23 Section 37.23 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  19. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... identification cards. 37.23 Section 37.23 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a)...

  20. 6 CFR 37.23 - Reissued REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Minimum Documentation, Verification, and Card Issuance Requirements § 37.23 Reissued REAL ID driver's licenses and identification cards. (a) State... duplicate driver's licenses and identification cards as defined in this rule, the State must...

  1. Documenting the Undocumented: A Review of the United States' First Municipal ID Program

    ERIC Educational Resources Information Center

    Lagunes, Paul F.; Levin, Brian M.; Ditlmann, Ruth K.

    2012-01-01

    The City of New Haven, CT, sought to promote the assimilation of its immigrants by becoming the first American city to provide a government-backed ID, the Elm City Resident Card, to all residents. This article tests the policy's effectiveness in commerce. We find that Hispanics/Latinos are asked to present an ID more than Whites/Anglos, showing…

  2. Id2a is required for hepatic outgrowth during liver development in zebrafish.

    PubMed

    Khaliq, Mehwish; Choi, Tae-Young; So, Juhoon; Shin, Donghun

    2015-11-01

    During development, inhibitor of DNA binding (Id) proteins, a subclass of the helix-loop-helix family of proteins, regulate cellular proliferation, differentiation, and apoptosis in various organs. However, a functional role of Id2a in liver development has not yet been reported. Here, using zebrafish as a model organism, we provide in vivo evidence that Id2a regulates hepatoblast proliferation and cell death during liver development. Initially, in the liver, id2a is expressed in hepatoblasts and after their differentiation, id2a expression is restricted to biliary epithelial cells. id2a knockdown in zebrafish embryos had no effect on hepatoblast specification or hepatocyte differentiation. However, liver size was greatly reduced in id2a morpholino-injected embryos, indicative of a hepatic outgrowth defect attributable to the significant decrease in proliferating hepatoblasts concomitant with the significant increase in hepatoblast cell death. Altogether, these data support the role of Id2a as an important regulator of hepatic outgrowth via modulation of hepatoblast proliferation and survival during liver development in zebrafish.

  3. Wicked ID: Conceptual Framework for Considering Instructional Design as a Wicked Problem

    ERIC Educational Resources Information Center

    Becker, Katrin

    2007-01-01

    The process of instructional design has parallels in other design disciplines. Software design is one that has experienced intense attention in the last 30 or so years, and many lessons learned there can be applied to ID. Using software design as a springboard, this concept paper seeks to propose a new approach to ID. It suggests that…

  4. 77 FR 33794 - True Product ID, Inc.; Order of Suspension of Trading

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION True Product ID, Inc.; Order of Suspension of Trading June 5, 2012. It appears to the Securities... securities of True Product ID, Inc. (``True Product'') because it has not filed a periodic report since...

  5. Regulation of Id2 expression by CCAAT/enhancer binding protein β

    PubMed Central

    Karaya, Kazuhiro; Mori, Seiichi; Kimoto, Hisashi; Shima, Yoko; Tsuji, Yoshihito; Kurooka, Hisanori; Akira, Shizuo; Yokota, Yoshifumi

    2005-01-01

    Mice deficient for Id2, a negative regulator of basic helix–loop–helix (bHLH) transcription factors, exhibit a defect in lactation due to impaired lobuloalveolar development during pregnancy, similar to the mice lacking the CCAAT enhancer binding protein (C/EBP) β. Here, we show that Id2 is a direct target of C/EBPβ. Translocation of C/EBPβ into the nucleus, which was achieved by using a system utilizing the fusion protein between C/EBPβ and the ligand-binding domain of the human estrogen receptor (C/EBPβ-ERT), demonstrated the rapid induction of endogenous Id2 expression. In reporter assays, transactivation of the Id2 promoter by C/EBPβ was observed and, among three potential C/EBPβ binding sites found in the 2.3 kb Id2 promoter region, the most proximal element was responsible for the transactivation. Electrophoretic mobility shift assay (EMSA) identified this element as a core sequence to which C/EBPβ binds. Chromatin immunoprecipitation (ChIP) furthermore confirmed the presence of C/EBPβ in the Id2 promoter region. Northern blotting showed that Id2 expression in C/EBPβ-deficient mammary glands was reduced at 10 days post coitus (d.p.c.), compared with that in wild-type mammary glands. Thus, our data demonstrate that Id2 is a direct target of C/EBPβ and provide insight into molecular mechanisms underlying mammary gland development during pregnancy. PMID:15809228

  6. Role of Id proteins in B lymphocyte activation: new insights from knockout mouse studies.

    PubMed

    Sugai, Manabu; Gonda, Hiroyuki; Nambu, Yukiko; Yokota, Yoshifumi; Shimizu, Akira

    2004-09-01

    Id (inhibitor of differentiation) proteins play important roles in cell differentiation, cell cycle control, and apoptosis. They act as negative regulators of basic helix-loop-helix-type transcription factors, which positively regulate differentiation of various cell types. Id proteins work to block B lymphocyte (B cell) maturation at an early differentiation step, as demonstrated by gain-of-function studies. In recent years a series of gene-targeted mice lacking different Ids have been generated. Analyses of these gene-targeted mice provide information useful for understanding the physiological roles of Ids in B cell biology. Id3 is required for proper B cell functions and acts by controlling the cell cycle. Upon B cell activation, Id2 acts as a negative regulator to prevent potentially harmful effects brought about by excessive immunological reactions; one of its special roles is to maintain low serum concentrations of immunoglobulin E (IgE). The Id2 protein does this by antagonizing E2A and Pax5 activities, both of which are required for proper B cell activation. This review presents several new insights into B cell differentiation and activation programs and the physiological role of Id proteins in B cell activation. PMID:15184986

  7. Rethinking Social Network Assessment for Students with Intellectual Disabilities (ID) in Postsecondary Education

    ERIC Educational Resources Information Center

    Eisenman, Laura T.; Farley-Ripple, Elizabeth; Culnane, Mary; Freedman, Brian

    2013-01-01

    Social networks of persons with intellectual disabilities (ID) have been characterized as smaller and less diverse than those of typical peers. Advocates have focused on strengthening those social networks by expanding circles of social support, protection, and friendship. As young adults with ID experience increasing levels of community…

  8. Security and Privacy Improvements for the Belgian eID Technology

    NASA Astrophysics Data System (ADS)

    Verhaeghe, Pieter; Lapon, Jorn; de Decker, Bart; Naessens, Vincent; Verslype, Kristof

    The Belgian Electronic Identity Card enables Belgian citizens to prove their identity digitally and to sign electronic documents. At the end of 2009, every Belgian citizen older than 12 years will have such an eID card. In the future, usage of the eID card may be mandatory. However, irresponsible use of the card may cause harm to individuals.

  9. The Numbers Game: Phasing in Generated ID Numbers at the University of Oregon

    ERIC Educational Resources Information Center

    Eveland, Sue

    2005-01-01

    With all the recent headlines about security breaches and information loss at financial and educational institutions, the higher education community needs to address the issue of using social security numbers as ID numbers. The University of Oregon undertook a change process to assign generated ID numbers to all records in their information…

  10. An ID Network System to Prepare for Global Environmental/Health Concerns

    NASA Astrophysics Data System (ADS)

    Asano, Shoichiro; Yoneda, Susumu

    Climate change and/or pandemics are global life threatening concerns. For verifying and utilizing monitored data for solving to the Climate Change concerns, a network system based on device ID would be proposed. In this paper, we review the recent standardization initiatives in ITU-T, and propose an ID network that can be used to verify the solutions.

  11. Ubiquitin-SUMO circuitry controls activated fanconi anemia ID complex dosage in response to DNA damage.

    PubMed

    Gibbs-Seymour, Ian; Oka, Yasuyoshi; Rajendra, Eeson; Weinert, Brian T; Passmore, Lori A; Patel, Ketan J; Olsen, Jesper V; Choudhary, Chunaram; Bekker-Jensen, Simon; Mailand, Niels

    2015-01-01

    We show that central components of the Fanconi anemia (FA) DNA repair pathway, the tumor suppressor proteins FANCI and FANCD2 (the ID complex), are SUMOylated in response to replication fork stalling. The ID complex is SUMOylated in a manner that depends on the ATR kinase, the FA ubiquitin ligase core complex, and the SUMO E3 ligases PIAS1/PIAS4 and is antagonized by the SUMO protease SENP6. SUMOylation of the ID complex drives substrate selectivity by triggering its polyubiquitylation by the SUMO-targeted ubiquitin ligase RNF4 to promote its removal from sites of DNA damage via the DVC1-p97 ubiquitin segregase complex. Deregulation of ID complex SUMOylation compromises cell survival following replication stress. Our results uncover a regulatory role for SUMOylation in the FA pathway, and we propose that ubiquitin-SUMO signaling circuitry is a mechanism that contributes to the balance of activated ID complex dosage at sites of DNA damage.

  12. Overestimation of the 25(OH)D serum concentration with the automated IDS EIA kit.

    PubMed

    Cavalier, Etienne; Huberty, Véronique; Cormier, Catherine; Souberbielle, Jean-Claude

    2011-02-01

    We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different "open" automated platforms. We performed a comparison between the IDS EIA kit adapted on two different "open"automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50-60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit.

  13. IDS crossing of the blood-brain barrier corrects CNS defects in MPSII mice.

    PubMed

    Polito, Vinicia Assunta; Cosma, Maria Pia

    2009-08-01

    Mucopolysaccharidosis type II (MPSII), or Hunter syndrome, arises from a deficiency in iduronate 2-sulfatase (IDS), and it is characterized by progressive somatic and neurological involvement. The MPSII mouse model reproduces the features of MPSII patients. Systemic administration of the AAV2/5CMV-hIDS vector in MPSII mouse pups results in the full correction of glycosaminoglycan (GAG) accumulation in visceral organs and in the rescue of the defects and GAG accumulation in the central nervous system (CNS). Remarkably, in treated MPSII animals, this CNS correction arises from the crossing of the blood-brain barrier by the IDS enzyme itself, not from the brain transduction. Thus, we show here that early treatment of MPSII mice with one systemic injection of AAV2/5CMV-hIDS results in prolonged and high levels of circulating IDS that can efficiently and simultaneously rescue both visceral and CNS defects for up to 18 months after therapy.

  14. Thymocyte selection is regulated by the helix-loop-helix inhibitor protein, Id3.

    PubMed

    Rivera, R R; Johns, C P; Quan, J; Johnson, R S; Murre, C

    2000-01-01

    E2A, HEB, E2-2, and daughterless are basic helix-loop-helix (bHLH) proteins that play key roles in multiple developmental pathways. The DNA binding activity of E2A, HEB, and E2-2 is regulated by a distinct class of inhibitor HLH proteins, the Id gene products. Here, we show that Id3 is required for major histocompatability (MHC) class I- and class II-restricted thymocyte positive selection. Additionally, H-Y TCR-mediated negative selection is severely perturbed in Id3 null mutant mice. Finally, we show that E2A and Id3 interact genetically to regulate thymocyte development. These observations identify the HLH inhibitory protein Id3 as an essential component required for proper thymocyte maturation.

  15. Molecular characterization of lactobacilli isolated from fermented idli batter

    PubMed Central

    Agaliya, Perumal Jayaprabha; Jeevaratnam, Kadirvelu

    2013-01-01

    Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was performed by RAPD, 16S rRNA analysis, 16S ARDRA, and Multiplex PCR for species identification. RAPD was carried out using the primer R2 and M13. Five different clusters were obtained based on RAPD indicating strain level variation. 16S rRNA analysis showed 99 to 100% homology towards Lactobacillus plantarum. The restriction digestion pattern was similar for all the isolates with the restriction enzyme AluI. The subspecies were identified by performing Multiplex PCR using species specific primer. Among the five clusters, three clusters were clearly identified as Lactobacillus plantarum subsp. plantarum, Lactobacillus pentosus, and Lactobacillus plantarum subsp. argentoratensis. PMID:24688512

  16. A neural-visualization IDS for honeynet data.

    PubMed

    Herrero, Álvaro; Zurutuza, Urko; Corchado, Emilio

    2012-04-01

    Neural intelligent systems can provide a visualization of the network traffic for security staff, in order to reduce the widely known high false-positive rate associated with misuse-based Intrusion Detection Systems (IDSs). Unlike previous work, this study proposes an unsupervised neural models that generate an intuitive visualization of the captured traffic, rather than network statistics. These snapshots of network events are immensely useful for security personnel that monitor network behavior. The system is based on the use of different neural projection and unsupervised methods for the visual inspection of honeypot data, and may be seen as a complementary network security tool that sheds light on internal data structures through visual inspection of the traffic itself. Furthermore, it is intended to facilitate verification and assessment of Snort performance (a well-known and widely-used misuse-based IDS), through the visualization of attack patterns. Empirical verification and comparison of the proposed projection methods are performed in a real domain, where two different case studies are defined and analyzed.

  17. Comparison of ID performance using real and synthetic imagery

    NASA Astrophysics Data System (ADS)

    Jacobs, Eddie L.; Edwards, Timothy C.; Miller, Brian; Hodgkin, Van A.

    2002-07-01

    Recent experiments performed at the U.S. Army Night Vision and Electronic Sensors Directorate (NVESD) provide significant insight into the validation of synthetic imagery for use in human perception experiments. This paper documents the procedures and results of target identification (ID) experiments using real and synthetic thermal imagery. Real imagery representing notional first generation and advanced scanning sensor systems was obtained. Parameters derived from the sensor data were used to generate synthetic imagery using the NVESD Paint the Night simulation. Both image sets were then used in a target identification experiment with trained human observers. Perception test results were analyzed and compared with metrics derived from the imagery. Several parameters missing from the original truth data were found to correlate with differences in the perception data. Synthetic data were regenerated using these additional parameters. A subsequent perception experiment confirmed the importance of these parameters, and a good match was obtained between real and synthetic imagery. While the techniques used in this series of experiments do not constitute a definitive method for validating synthetic imagery, they point to some important observations on validation. The main observation is that both target and local background characteristics must be sufficiently specified in the truth data in order to obtain good agreement between synthetic and real data. The paper concludes with suggestions as to the level of detail necessary for truth data when using synthetic imagery in perception experiments.

  18. Molecular characterization of lactobacilli isolated from fermented idli batter.

    PubMed

    Agaliya, Perumal Jayaprabha; Jeevaratnam, Kadirvelu

    2013-12-01

    Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was performed by RAPD, 16S rRNA analysis, 16S ARDRA, and Multiplex PCR for species identification. RAPD was carried out using the primer R2 and M13. Five different clusters were obtained based on RAPD indicating strain level variation. 16S rRNA analysis showed 99 to 100% homology towards Lactobacillus plantarum. The restriction digestion pattern was similar for all the isolates with the restriction enzyme AluI. The subspecies were identified by performing Multiplex PCR using species specific primer. Among the five clusters, three clusters were clearly identified as Lactobacillus plantarum subsp. plantarum, Lactobacillus pentosus, and Lactobacillus plantarum subsp. argentoratensis.

  19. Six steps in quality intervention development (6SQuID)

    PubMed Central

    Wight, Daniel; Wimbush, Erica; Jepson, Ruth; Doi, Lawrence

    2016-01-01

    Improving the effectiveness of public health interventions relies as much on the attention paid to their design and feasibility as to their evaluation. Yet, compared to the vast literature on how to evaluate interventions, there is little to guide researchers or practitioners on how best to develop such interventions in practical, logical, evidence based ways to maximise likely effectiveness. Existing models for the development of public health interventions tend to have a strong social-psychological, individual behaviour change orientation and some take years to implement. This paper presents a pragmatic guide to six essential Steps for Quality Intervention Development (6SQuID). The focus is on public health interventions but the model should have wider applicability. Once a problem has been identified as needing intervention, the process of designing an intervention can be broken down into six crucial steps: (1) defining and understanding the problem and its causes; (2) identifying which causal or contextual factors are modifiable: which have the greatest scope for change and who would benefit most; (3) deciding on the mechanisms of change; (4) clarifying how these will be delivered; (5) testing and adapting the intervention; and (6) collecting sufficient evidence of effectiveness to proceed to a rigorous evaluation. If each of these steps is carefully addressed, better use will be made of scarce public resources by avoiding the costly evaluation, or implementation, of unpromising interventions. PMID:26573236

  20. Minimum wafer thickness by rotated ingot ID wafering. [Inner Diameter

    NASA Technical Reports Server (NTRS)

    Chen, C. P.; Leipold, M. H.

    1984-01-01

    The efficient utilization of materials is critical to certain device applications such as silicon for photovoltaics or diodes and gallium-gadolinium-garnet for memories. A variety of slicing techniques has been investigated to minimize wafer thickness and wafer kerf. This paper presents the results of analyses of ID wafering of rotated ingots based on predicted fracture behavior of the wafer as a result of forces during wafering and the properties of the device material. The analytical model indicated that the minimum wafer thickness is controlled by the depth of surface damage and the applied cantilever force. Both of these factors should be minimized. For silicon, a minimum thickness was found to be approximately 200 x 10 - 6th m for conventional sizes of rotated ingot wafering. Fractures through the thickness of the wafer rather than through the center supporting column were found to limit the minimum wafer thickness. The model suggested that the use of a vacuum chuck on the wafer surface to enhance cleavage fracture of the center supporting core and, with silicon, by using 111-line-type ingots could have potential for reducing minimum wafer thickness.

  1. Pros and Cons of ID vs. 3D Modeling

    NASA Technical Reports Server (NTRS)

    Klimchuk, James A.

    2012-01-01

    Advances in computing capability have led to tremendous improvements in 3D modeling. Entire active regions are being simulated in what might be described as a first principles way, in which plasma heating is treated self consistently rather than through the specification of heating functions. There are limitations to this approach, however, as actual heating mechanisms on the Sun involve spatial scales orders of magnitude smaller than what these simulations can resolve. Other simulations begin to resolve these scales, but they only treat a tiny volume and do not include the all important coupling with larger scales or with other parts of the atmosphere, and so cannot be readily compared with observations. Finally, ID hydrodynamic models capture the field-aligned evolution of the plasma extremely well and are ideally suited for data comparison, but they treat the heating in a totally ad hoc manner. All of these approaches have important contributions to make, but we must be aware of their limitations. I will highlight some of the strengths. and weaknesses of each.

  2. Protocol for VOC-Arid ID remediation performance characterization

    SciTech Connect

    Tegner, B.J.; Hassig, N.L.; Last, G.V.

    1994-09-01

    The Volatile Organic Compound-Arid Integrated Demonstration (VOC-Arid ID) is a technology development program sponsored by the US Department of Energy`s Office of Technology Development that is targeted to acquire, develop, demonstrate, and deploy new technologies for the remediation of VOC contaminants in the soils and groundwaters of arid DOE sites. Technologies cannot be adequately evaluated unless sufficient site characterization and technology performance data have been collection and analyzed. The responsibility for identifying these data needs has been placed largely on the Principal Investigators (PIs) developing the remediation technology, who usually are not experts in site characterization or in identification of appropriate sampling, analysis, and monitoring techniques to support the field testing. This document provides a protocol for planning the collection of data before, during, and after a test of a new technology. This generic protocol provides the PIs and project managers with a set of steps to follow. The protocol is based on a data collection planning process called the Data Quality Objectives (DQO) process, which was originally developed by the US Environmental Protection Agency and has been expanded by DOE to support site cleanup decisions. The DQO process focuses on the quality and quantity of data required to make decision. Stakeholders to the decisions must negotiate such key inputs to the process as the decision rules that will be used and the acceptable probabilities of making decision errors.

  3. Molecular characterization of lactobacilli isolated from fermented idli batter.

    PubMed

    Agaliya, Perumal Jayaprabha; Jeevaratnam, Kadirvelu

    2013-12-01

    Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was performed by RAPD, 16S rRNA analysis, 16S ARDRA, and Multiplex PCR for species identification. RAPD was carried out using the primer R2 and M13. Five different clusters were obtained based on RAPD indicating strain level variation. 16S rRNA analysis showed 99 to 100% homology towards Lactobacillus plantarum. The restriction digestion pattern was similar for all the isolates with the restriction enzyme AluI. The subspecies were identified by performing Multiplex PCR using species specific primer. Among the five clusters, three clusters were clearly identified as Lactobacillus plantarum subsp. plantarum, Lactobacillus pentosus, and Lactobacillus plantarum subsp. argentoratensis. PMID:24688512

  4. Mono County update

    SciTech Connect

    Lyster, D.L.

    1987-06-01

    On February 9, 1988, the Mono County Board of Supervisors voted to approve Bonneville Pacific Corporation's Mammoth Chance Geothermal Project. The project is an air-cooled, binary, geothermal power plant, 10 megawatts, net. The Mono County Board of Supervisors issued a project use-permit with vigorous and stringent conditions. Specific emphasis was placed on the establishment of a monitoring program designed to detect the effects of geothermal development on the springs at the Hot Creek Fish Hatchery and Hot Creek Gorge. On October 5, 1987, the Mono County Planning Commission granted a use-permit to Mammoth Pacific for its Mammoth Pacific II Project, a binary, air-cooled, geothermal power plant, 10 megawatts, net. The issuance of the use-permit instigated an appeal by the Sierra Club. That appeal was heard on February 22, 1988, At the end of the testimony, the Board of Supervisors voted to uphold the appeal of the Sierra Club, thereby denying the project by a vote of 3 to 2. The main areas of concern voiced by the majority of the Board included potential hydrologic impacts to Hot Creek Gorge and Hot Creek Fish Hatchery, visual impacts, and impacts to mule deer migration and survival. One of the options now available to Mammoth Pacific is to request that the project be denied without prejudice. This would allow Mammoth Pacific to return to the Board immediately with additional material regarding its concerns.

  5. 77 FR 73005 - Revisions to the California State Implementation Plan, Imperial County, Placer County, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-07

    ... County, and Ventura County Air Pollution Control Districts AGENCY: Environmental Protection Agency (EPA... Pollution Control District (ICAPCD), Placer County Air Pollution Control District (PCAPCD) and Ventura County Air Pollution Control District (PCAPCD) portions of the California State Implementation Plan...

  6. Id1 and Id3 co-expression correlates with clinical outcome in stage III-N2 non-small cell lung cancer patients treated with definitive chemoradiotherapy

    PubMed Central

    2013-01-01

    Background Inhibitor of DNA binding 1 (Id1) and 3 (Id3) genes have been related with the inhibition of cell differentiation, cell growth promotion and tumor metastasis. Recently, Id1 has been identified as an independent prognostic factor in patients with lung adenocarcinoma, regardless of the stage. Furthermore, Id1 may confer resistance to treatment (both, radiotherapy and chemotherapy). Methods We have studied, using monoclonal antibodies for immunohistochemistry, the Id1 and Id3 tumor epithelial expression in 17 patients with stage III-N2 non-small cell lung cancer (NSCLC) treated with definitive chemoradiotherapy. Results Id1 expression is observed in 82.4% of the tumors, whereas Id3 expression is present in 41.2% of the samples. Interestingly, Id1 and Id3 expression are mutually correlated (R = 0.579, p = 0.015). In a subgroup analysis of patients with the most locally advanced disease (T4N2 stage), co-expression of Id1 and Id3 showed to be related with a worse overall survival (45 vs 6 months, p = 0.002). A trend towards significance for a worse progression free survival (30 vs 1 months, p = 0.219) and a lower response rate to the treatment (RR = 50% vs 87.5%, p = 0.07) were also observed. Conclusions A correlation between Id1 and Id3 protein expression is observed. Id1 and Id3 co-expression seems associated with a poor clinical outcome in patients with locally advanced NSCLC treated with definitive chemoradiotherapy. PMID:23311395

  7. Substance use and misuse in persons with intellectual disabilities (ID): results of a survey in ID and addiction services in Flanders.

    PubMed

    To, Wing Ting; Neirynck, Soetkin; Vanderplasschen, Wouter; Vanheule, Stijn; Vandevelde, Stijn

    2014-01-01

    Little is known about the characteristics of substance users with intellectual disabilities (ID). Nevertheless, this group is assumed to be at greater risk of developing substance misuse problems. This study focuses on substance users and misusers with ID, and investigates whether the two groups differ significantly in terms of the nature and consequences of their substance (mis)use. Information regarding the characteristics of the substance (mis)users, the substances used, the negative consequences of substance (mis)use, and the service use was collected through a questionnaire forwarded to ID and addiction services in Flanders. Caregivers identified 104 substance users and misusers with ID. Overall, few differences were observed between users and misusers. This finding underscores that substance use in persons with ID can have important consequences. Substance misusers, however, were found to have more mood changes, more suicidal ideation/thoughts, and more negative long-term consequences on their health, daily activity, and relationships due to substance misuse. Substance use and misuse were associated with mental health problems and were suggested to be a risk factor for offending behavior. To provide appropriate support for this specific population, an individualized approach is suggested that supports better intersectoral collaboration between services.

  8. Unconventional myosin ID is expressed in myelinating oligodendrocytes.

    PubMed

    Yamazaki, Reiji; Ishibashi, Tomoko; Baba, Hiroko; Yamaguchi, Yoshihide

    2014-10-01

    Myelin is a dynamic multilamellar structure that ensheathes axons and is crucial for normal neuronal function. In the central nervous system (CNS), myelin is produced by oligodendrocytes that wrap many layers of plasma membrane around axons. The dynamic membrane trafficking system, which relies on motor proteins, is required for myelin formation and maintenance. Previously, we found that myosin ID (Myo1d), a class I myosin, is enriched in the rat CNS myelin fraction. Myo1d is an unconventional myosin and has been shown to be involved in membrane trafficking in the recycling pathway in an epithelial cell line. Western blotting revealed that Myo1d expression begins early in myelinogenesis and continues to increase into adulthood. The localization of Myo1d in CNS myelin has not been reported, and the function of Myo1d in vivo remains unknown. To demonstrate the expression of Myo1d in CNS myelin and to begin to explore the function of Myo1d in myelination, we produced a new antibody against Myo1d that has a high titer and specificity for rat Myo1d. By using this antibody, we demonstrated that Myo1d is expressed in rat CNS myelin and is especially abundant in abaxonal and adaxonal regions (the outer and inner cytoplasm-containing loops, respectively), but that expression is low in peripheral nervous system myelin. In culture, Myo1d was expressed in mature rat oligodendrocytes. Furthermore, an increase in expression of Myo1d during maturation of CNS white matter (cerebellum and corpus callosum) was demonstrated by histological analysis. These results suggest that Myo1d may be involved in the formation and/or maintenance of CNS myelin.

  9. The ID23-2 structural biology microfocus beamline at the ESRF

    PubMed Central

    Flot, David; Mairs, Trevor; Giraud, Thierry; Guijarro, Matias; Lesourd, Marc; Rey, Vicente; van Brussel, Denis; Morawe, Christian; Borel, Christine; Hignette, Olivier; Chavanne, Joel; Nurizzo, Didier; McSweeney, Sean; Mitchell, Edward

    2010-01-01

    The first phase of the ESRF beamline ID23 to be constructed was ID23-1, a tunable MAD-capable beamline which opened to users in early 2004. The second phase of the beamline to be constructed is ID23-2, a monochromatic microfocus beamline dedicated to macromolecular crystallography experiments. Beamline ID23-2 makes use of well characterized optical elements: a single-bounce silicon (111) monochromator and two mirrors in Kirkpatrick–Baez geometry to focus the X-ray beam. A major design goal of the ID23-2 beamline is to provide a reliable, easy-to-use and routine microfocus beam. ID23-2 started operation in November 2005, as the first beamline dedicated to microfocus macromolecular crystallography. The beamline has taken the standard automated ESRF macromolecular crystallography environment (both hardware and software), allowing users of ID23-2 to be rapidly familiar with the microfocus environment. This paper describes the beamline design, the special considerations taken into account given the microfocus beam, and summarizes the results of the first years of the beamline operation. PMID:20029119

  10. Bio-fortification and shelf-life extension of idli batter using curry leaves (Murraya koenigii).

    PubMed

    Chelliah, R; Ramakrishnan, S R; Premkumar, D; Antony, U

    2016-06-01

    Among several traditional foods of India, idli is one of the most popular and commonly consumed steamed products. A new method of adding Murraya koenigii (curry leaves) to idli batter as a vehicle for fortification and extension of shelf-life has been developed. Dried curry leaves powder was incorporated with other ingredients like rice and dehusked black gram in different proportions to optimize the most palatable formulation. Rate of fermentation and microbial changes in the batter; nutritional qualities, texture and sensory properties of the prepared product were assessed. Incorporation of curry leaves powder (5 %) in idli batter increased the shelf-life and also increased the flavour, texture and appearance of the idli. The calcium content of the prepared idli was 10 times more than that of the control idli, while dietary fiber content increased by 18.6 %. Anti-microbial activity of the curry leaves in idli batter extended the shelf-life from 2 to 5 days when stored at 30 °C.

  11. SpDamID: Marking DNA Bound by Protein Complexes Identifies Notch-Dimer Responsive Enhancers

    PubMed Central

    Hass, Matthew R.; Liow, Hien-haw; Chen, Xiaoting; Sharma, Ankur; Inoue, Yukiko U.; Inoue, Takayoshi; Reeb, Ashley; Martens, Andrew; Fulbright, Mary; Raju, Saravanan; Stevens, Michael; Boyle, Scott; Park, Joo-Seop; Weirauch, Matthew T.; Brent, Michael; Kopan, Raphael

    2015-01-01

    SUMMARY We developed Split DamID (SpDamID), a protein complementation version of DamID, to mark genomic DNA bound in vivo by interacting or juxtapositioned transcription factors. Inactive halves of DAM (DNA Adenine Methyltransferase) were fused to protein pairs to be queried Interaction or proximity enabled DAM reconstitution and methylation of adenine in GATC. Inducible SpDamID was used to analyze Notch-mediated transcriptional activation. We demonstrate that Notch complexes label RBP sites broadly across the genome, and show that a subset of these complexes that recruit MAML and p300 undergo changes in chromatin accessibility in response to Notch signaling. SpDamID differentiates between monomeric and dimeric binding thereby allowing for identification of half-site motifs used by Notch dimers. Motif enrichment of Notch enhancers coupled with SpDamID reveals co-targeting of regulatory sequences by Notch and Runx1. SpDamID represents a sensitive and powerful tool that enables dynamic analysis of combinatorial protein-DNA transactions at a genome-wide level. PMID:26257285

  12. Bio-fortification and shelf-life extension of idli batter using curry leaves (Murraya koenigii).

    PubMed

    Chelliah, R; Ramakrishnan, S R; Premkumar, D; Antony, U

    2016-06-01

    Among several traditional foods of India, idli is one of the most popular and commonly consumed steamed products. A new method of adding Murraya koenigii (curry leaves) to idli batter as a vehicle for fortification and extension of shelf-life has been developed. Dried curry leaves powder was incorporated with other ingredients like rice and dehusked black gram in different proportions to optimize the most palatable formulation. Rate of fermentation and microbial changes in the batter; nutritional qualities, texture and sensory properties of the prepared product were assessed. Incorporation of curry leaves powder (5 %) in idli batter increased the shelf-life and also increased the flavour, texture and appearance of the idli. The calcium content of the prepared idli was 10 times more than that of the control idli, while dietary fiber content increased by 18.6 %. Anti-microbial activity of the curry leaves in idli batter extended the shelf-life from 2 to 5 days when stored at 30 °C. PMID:27478242

  13. Hydrology of Lake County, Florida

    USGS Publications Warehouse

    Knochenmus, Darwin D.; Hughes, G.H.

    1976-01-01

    Lake County includes a 1,150 square-mile area consisting of ridges, uplands, and valleys in central-peninsular Florida. About 32 percent of the county is covered by lakes, swamps, and marshes. Water requirements in 1970 averaged about 54 million gallons per day. About 85 percent of the water was obtained from wells; about 15 percent from lakes. The Floridan aquifer supplies almost all the ground water used in Lake County. Annual recharge to the Floridan aquifer averages about 7 inches over the county; runoff average 8.5 inches. The quality of ground and surface water in Lake County is in general good enough for most uses; however, the poor quality of Floridan-aquifer water in the St. John River Valley probably results from the upward movement of saline water along a fault zone. Surface water in Lake County is usually less mineralized than ground water but is more turbid and colored. (Woodard-USGS)

  14. Id1 expression promotes T regulatory cell differentiation by facilitating TCR costimulation.

    PubMed

    Liu, Chen; Wang, Hong-Cheng; Yu, Sen; Jin, Rong; Tang, Hui; Liu, Yuan-Feng; Ge, Qing; Sun, Xiao-Hong; Zhang, Yu

    2014-07-15

    T regulatory (Treg) cells play crucial roles in the regulation of cellular immunity. The development of Treg cells depends on signals from TCRs and IL-2Rs and is influenced by a variety of transcription factors. The basic helix-loop-helix proteins are known to influence TCR signaling thresholds. Whether this property impacts Treg differentiation is not understood. In this study, we interrogated the role of basic helix-loop-helix proteins in the production of Treg cells using the CD4 promoter-driven Id1 transgene. We found that Treg cells continued to accumulate as Id1 transgenic mice aged, resulting in a significant increase in Treg cell counts in the thymus as well as in the periphery compared with wild-type controls. Data from mixed bone marrow assays suggest that Id1 acts intrinsically on developing Treg cells. We made a connection between Id1 expression and CD28 costimulatory signaling because Id1 transgene expression facilitated the formation of Treg precursors in CD28(-/-) mice and the in vitro differentiation of Treg cells on thymic dendritic cells despite the blockade of costimulation by anti-CD80/CD86. Id1 expression also allowed in vitro Treg differentiation without anti-CD28 costimulation, which was at least in part due to enhanced production of IL-2. Notably, with full strength of costimulatory signals, however, Id1 expression caused modest but significant suppression of Treg induction. Finally, we demonstrate that Id1 transgenic mice were less susceptible to the induction of experimental autoimmune encephalomyelitis, thus illustrating the impact of Id1-mediated augmentation of Treg cell levels on cellular immunity.

  15. The metalloid arsenite induces nuclear export of Id3 possibly via binding to the N-terminal cysteine residues

    SciTech Connect

    Kurooka, Hisanori; Sugai, Manabu; Mori, Kentaro; Yokota, Yoshifumi

    2013-04-19

    Highlights: •Sodium arsenite induces cytoplasmic accumulation of Id3. •Arsenite binds to closely spaced N-terminal cysteine residues of Id3. •N-terminal cysteines are essential for arsenite-induced nuclear export of Id3. •Nuclear export of Id3 counteracts its transcriptional repression activity. -- Abstract: Ids are versatile transcriptional repressors that regulate cell proliferation and differentiation, and appropriate subcellular localization of the Id proteins is important for their functions. We previously identified distinct functional nuclear export signals (NESs) in Id1 and Id2, but no active NES has been reported in Id3. In this study, we found that treatment with the stress-inducing metalloid arsenite led to the accumulation of GFP-tagged Id3 in the cytoplasm. Cytoplasmic accumulation was impaired by a mutation in the Id3 NES-like sequence resembling the Id1 NES, located at the end of the HLH domain. It was also blocked by co-treatment with the CRM1-specific nuclear export inhibitor leptomycin B (LMB), but not with the inhibitors for mitogen-activated protein kinases (MAPKs). Importantly, we showed that the closely spaced N-terminal cysteine residues of Id3 interacted with the arsenic derivative phenylarsine oxide (PAO) and were essential for the arsenite-induced cytoplasmic accumulation, suggesting that arsenite induces the CRM1-dependent nuclear export of Id3 via binding to the N-terminal cysteines. Finally, we demonstrated that Id3 significantly repressed arsenite-stimulated transcription of the immediate-early gene Egr-1 and that this repression activity was inversely correlated with the arsenite-induced nuclear export. Our results imply that Id3 may be involved in the biological action of arsenite.

  16. Id-1 gene and gene products as therapeutic targets for treatment of breast cancer and other types of carcinoma

    DOEpatents

    Desprez, Pierre-Yves; Campisi, Judith

    2014-08-19

    A method for treatment of breast cancer and other types of cancer. The method comprises targeting and modulating Id-1 gene expression, if any, for the Id-1 gene, or gene products in breast or other epithelial cancers in a patient by delivering products that modulate Id-1 gene expression. When expressed, Id-1 gene is a prognostic indicator that cancer cells are invasive and metastatic.

  17. Improvement and analysis of ID3 algorithm in decision-making tree

    NASA Astrophysics Data System (ADS)

    Xie, Xiao-Lan; Long, Zhen; Liao, Wen-Qi

    2015-12-01

    For the cooperative system under development, it needs to use the spatial analysis and relative technology concerning data mining in order to carry out the detection of the subject conflict and redundancy, while the ID3 algorithm is an important data mining. Due to the traditional ID3 algorithm in the decision-making tree towards the log part is rather complicated, this paper obtained a new computational formula of information gain through the optimization of algorithm of the log part. During the experiment contrast and theoretical analysis, it is found that IID3 (Improved ID3 Algorithm) algorithm owns higher calculation efficiency and accuracy and thus worth popularizing.

  18. Wnt/{beta}-catenin signaling changes C2C12 myoblast proliferation and differentiation by inducing Id3 expression

    SciTech Connect

    Zhang, Long; Shi, Songting; Zhang, Juan; Zhou, Fangfang; Dijke, Peter ten

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer Expression of Id3 but not Id1 is induced by Wnt3a stimulation in C2C12 cells. Black-Right-Pointing-Pointer Wnt3a induces Id3 expression via canonical Wnt/{beta}-catenin pathway. Black-Right-Pointing-Pointer Wnt3a-induced Id3 expression does not depend on BMP signaling activation. Black-Right-Pointing-Pointer Induction of Id3 expression is critical determinant in Wnt3a-induced cell proliferation and differentiation. -- Abstract: Canonical Wnt signaling plays important roles in regulating cell proliferation and differentiation. In this study, we report that inhibitor of differentiation (Id)3 is a Wnt-inducible gene in mouse C2C12 myoblasts. Wnt3a induced Id3 expression in a {beta}-catenin-dependent manner. Bone morphogenetic protein (BMP) also potently induced Id3 expression. However, Wnt-induced Id3 expression occurred independent of the BMP/Smad pathway. Functional studies showed that Id3 depletion in C2C12 cells impaired Wnt3a-induced cell proliferation and alkaline phosphatase activity, an early marker of osteoblast cells. Id3 depletion elevated myogenin induction during myogenic differentiation and partially impaired Wnt3a suppressed myogenin expression in C2C12 cells. These results suggest that Id3 is an important Wnt/{beta}-catenin induced gene in myoblast cell fate determination.

  19. Family Impact in Intellectual Disability, Severe Mental Health Disorders and Mental Health Disorders in ID. A Comparison

    ERIC Educational Resources Information Center

    Martorell, Almudena; Gutierrez-Recacha, Pedro; Irazabal, Marcia; Marsa, Ferran; Garcia, Mercedes

    2011-01-01

    Family impact (or family burden) is a concept born in the field of mental health that has successfully been exported to the ambit of intellectual disability (ID). However, differences in family impact associated with severe mental health disorders (schizophrenia), to ID or to mental health problems in ID should be expected. Seventy-two adults with…

  20. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID driver's licenses and identification cards. (a) Driver's... individuals born after December 1, 1964, unless such license or card is a REAL ID-compliant driver's...

  1. 6 CFR 37.5 - Validity periods and deadlines for REAL ID driver's licenses and identification cards.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Validity periods and deadlines for REAL ID... SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS General § 37.5 Validity periods and deadlines for REAL ID driver's licenses and identification cards. (a)...

  2. Sexual abuse involving children with an intellectual disability (ID): a narrative review.

    PubMed

    Wissink, Inge B; van Vugt, Eveline; Moonen, Xavier; Stams, Geert-Jan J M; Hendriks, Jan

    2015-01-01

    The current paper provides a narrative review of the literature on sexual abuse, involving children with Intellectual Disability (ID). The thirteen articles that were found and met our criteria vary in their definitions of sexual abuse and in how ID was determined. Still, they do paint a general picture concerning (1) the extent of sexual abuse, (2) the nature of the sexual abuse, and (3) the institutional reactions following sexual abuse of children with ID. Our findings confirm the greater vulnerability of children with ID to become involved in sexual abuse both as a victim and as a perpetrator, and we discuss ways to help strengthening prevention and intervention methods. Nevertheless, more research is needed, as it is still a rather unexplored topic, which is striking in light of the high vulnerability of this group.

  3. Identification of an alternative transcript from the human iduronate-2-sulfatase (IDS) gene

    SciTech Connect

    Malmgren, H.; Carlberg, B.M.; Petterson, U.

    1995-09-01

    Iduronate-2-sulfatase (IDS) is involved in the degradation of heparan sulfate and dermatan sulfate in the lysosomes, and a deficiency in this enzyme results in Hunter syndrome. A 2.3-kb cDNA clone that contains the entire coding sequence of IDS has previously been reported. Here we describe the identification of a 1.4-kb transcript that may encode an IDS-like enzyme. The predicted protein is identical to the previously described enzyme, except for the absence of the 207-amino-acid COOH-terminal domain, which is replaced by 7 amino acids. Our data suggest that there might exist an additional form of the IDS enzyme in humans. The results from this study may have implications for the pathogenesis of the Hunter syndrome. 16 refs., 2 figs.

  4. Id2 IS REQUIRED FOR SPECIFICATION OF DOPAMINERGIC NEURONS DURING ADULT OLFACTORY NEUROGENESIS

    PubMed Central

    Havrda, Matthew C.; Harris, Brent T.; Mantani, Akio; Ward, Nora M.; Paolella, Brenton R.; Cuzon, Verginia C.; Yeh, Hermes H.; Israel, Mark A.

    2009-01-01

    Understanding the biology of adult neural stem cells has important implications for nervous system development and may contribute to our understanding of neurodegenerative disorders and their treatment. We have characterized the process of olfactory neurogenesis in adult mice lacking Inhibitor of DNA Binding 2 (Id2). We found a diminished olfactory bulb containing reduced numbers of granular and periglomerular neurons with a distinct paucity of dopaminergic periglomerular neurons. While no deficiency of the stem cell compartment was detectable, migrating neuroblasts in Id2−/− mutant mice prematurely undergo astroglial differentiation within a disorganized rostral migratory stream. Further, when evaluated in vitro loss of Id2 results in decreased proliferation of neural progenitors and decreased expression of the Hes1 and Mash1 transcription factors, known mediators of neuronal differentiation. These data support a novel role for sustained Id2 expression in migrating neural progenitors mediating olfactory dopaminergic neuronal differentiation in adult animals. PMID:19109490

  5. Id3 inhibits B lymphocyte progenitor growth and survival in response to TGF-beta.

    PubMed

    Kee, B L; Rivera, R R; Murre, C

    2001-03-01

    E proteins function in many developmental processes and are essential for the formation of lymphocyte progenitors. However, it is not known whether E proteins regulate lymphocyte survival, proliferation or differentiation or how their activity is regulated during lymphocyte development. We show here a role for Id3, an inhibitor of E protein activity, in the induction of apoptosis and growth arrest. Id3 is induced in response to transforming growth factor beta (TGF-beta), a pleiotropic cytokine that inhibits the growth and survival of normal and transformed lymphocyte progenitors. In the absence of Id3, the response of lymphocyte progenitors to TGF-beta is perturbed, which indicates that Id3 is a mediator of this response. Our data show a key role for E proteins in lymphocyte survival and link the activity of E proteins, and their antagonists, to members of the TGF-beta family of cytokines.

  6. 78 FR 68467 - Notice of Public Meetings, Twin Falls District Resource Advisory Council, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-14

    ... Resource Advisory Council (RAC) and subcommittee for the Greater sage-grouse will meet as indicated below... sage-grouse will meet at the Twin Falls District BLM Office, 2536 Kimberly Road, Twin Falls, ID...

  7. 77 FR 55813 - Transition of DOE-ID Public Reading Room

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... Library at 1776 Science Center Drive, Idaho Falls, ID 83401, beginning September 1, 2012. Access to....ntis.gov . DATES: Effective September 1, 2012. ADDRESSES: INL Research Library, 1776 Science...

  8. Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase

    PubMed Central

    Xu, Kaiming; Wang, Lanfang; Feng, Wei; Feng, Yue; Shu, Hui-Kuo G.

    2016-01-01

    Id1 is a helix-loop-helix transcriptional modulator that increases the aggressiveness of malignant glial neoplasms. Since most glioblastomas (GBMs) show increased phosphatidylinositol-3 kinase (PI-3K) signaling, we sought to determine whether this pathway regulates Id1 expression. Higher basal Id1 expression correlates with dysregulated PI-3K signaling in multiple established GBM cell lines. Further characterization of PI-3K-dependent Id1 regulation reveals that chemical or genetic inhibition of PI-3K signaling reduces Id1 protein but not mRNA expression. Overall, PI-3K signaling appears to enhance Id1 translation with no significant effect on its stability. PI-3K signaling is known to regulate protein translation through mTORC1-dependent phosphorylation of 4E-BP1, which reduces its association with and inhibition of the translation initiation factor eIF4E. Interestingly, while inhibition of PI-3K and AKT lowers 4E-BP1 phosphorylation and expression of Id1 in all cases, inhibition of TORC1 with rapamycin does not consistently have a similar effect suggesting an alternative mechanism for PI-3K-dependent regulation of Id1 translation. We now identify a potential role for the serine-threonine phosphatase PPM1G in translational regulation of Id1 protein expression. PPM1G knockdown by siRNA increase both 4E-BP1 phosphorylation and Id1 expression and PPM1G and 4E-BP1 co-associates in GBM cells. Furthermore, PPM1G is a phosphoprotein and this phosphorylation appears to be regulated by PI-3K activity. Finally, PI-3K inhibition increases PPM1G activity when assessed by an in vitro phosphatase assay. Our findings provide the first evidence that the PI-3K/AKT signaling pathway modulates PPM1G activity resulting in a shift in the balance between hyper- and hypo-phosphorylated 4E-BP1 and translational regulation of Id1 expression. PMID:27065332

  9. Equity and County College Financing.

    ERIC Educational Resources Information Center

    New Jersey State Commission on Financing Postsecondary Education, Trenton.

    An analysis by the Commission on Financial Postsecondary Education of the system by which New Jersey supports county colleges revealed disparities and inequities among the New Jersey counties in wealth and effort per full-time equivalent student, and current state financing practices that aggravate this problem. In response, alternative state…

  10. Frederick County Community Perception Survey.

    ERIC Educational Resources Information Center

    Frederick Community Coll., MD.

    In 1997, Frederick Community College (FCC) in Maryland conducted a telephone survey of a random sample of 466 Frederick County residents to identify their perceptions of the college. In particular, the survey examined Frederick County residents' image of FCC, level of awareness of services and programs offered by FCC, and the types of services…

  11. Topics for Lehigh County Seniors.

    ERIC Educational Resources Information Center

    Lehigh County Community Coll., Schnecksville, PA.

    Lehigh County Community College (Pennsylvania) and the Lehigh County Senior Citizens' Center collaborated on a project to assess the learning needs of the senior center's 1,600 members. A needs assessment completed by 68 center members and interviews of an additional 38 center members established that senior citizens preferred short-term workshops…

  12. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria.

    PubMed

    Davis Ii, Edward W; Weisberg, Alexandra J; Tabima, Javier F; Grunwald, Niklaus J; Chang, Jeff H

    2016-01-01

    Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/. PMID:27547538

  13. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria

    PubMed Central

    Tabima, Javier F.; Grunwald, Niklaus J.

    2016-01-01

    Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/. PMID:27547538

  14. EcID. A database for the inference of functional interactions in E. coli.

    PubMed

    Andres Leon, Eduardo; Ezkurdia, Iakes; García, Beatriz; Valencia, Alfonso; Juan, David

    2009-01-01

    The EcID database (Escherichia coli Interaction Database) provides a framework for the integration of information on functional interactions extracted from the following sources: EcoCyc (metabolic pathways, protein complexes and regulatory information), KEGG (metabolic pathways), MINT and IntAct (protein interactions). It also includes information on protein complexes from the two E. coli high-throughput pull-down experiments and potential interactions extracted from the literature using the web services associated to the iHOP text-mining system. Additionally, EcID incorporates results of various prediction methods, including two protein interaction prediction methods based on genomic information (Phylogenetic Profiles and Gene Neighbourhoods) and three methods based on the analysis of co-evolution (Mirror Tree, In Silico 2 Hybrid and Context Mirror). EcID associates to each prediction a specifically developed confidence score. The two main features that make EcID different from other systems are the combination of co-evolution-based predictions with the experimental data, and the introduction of E. coli-specific information, such as gene regulation information from EcoCyc. The possibilities offered by the combination of the EcID database information are illustrated with a prediction of potential functions for a group of poorly characterized genes related to yeaG. EcID is available online at http://ecid.bioinfo.cnio.es.

  15. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria.

    PubMed

    Davis Ii, Edward W; Weisberg, Alexandra J; Tabima, Javier F; Grunwald, Niklaus J; Chang, Jeff H

    2016-01-01

    Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/.

  16. Type II arginine methyltransferase PRMT5 regulates gene expression of inhibitors of differentiation/DNA binding Id2 and Id4 during glial cell differentiation.

    PubMed

    Huang, Jinghan; Vogel, Gillian; Yu, Zhenbao; Almazan, Guillermina; Richard, Stéphane

    2011-12-30

    PRMT5 is a type II protein arginine methyltranferase that catalyzes monomethylation and symmetric dimethylation of arginine residues. PRMT5 is functionally involved in a variety of biological processes including embryo development and circadian clock regulation. However, the role of PRMT5 in oligodendrocyte differentiation and central nervous system myelination is unknown. Here we show that PRMT5 expression gradually increases throughout postnatal brain development, coinciding with the period of active myelination. PRMT5 expression was observed in neurons, astrocytes, and oligodendrocytes. siRNA-mediated depletion of PRMT5 in mouse primary oligodendrocyte progenitor cells abrogated oligodendrocyte differentiation. In addition, the PRMT5-depleted oligodendrocyte progenitor and C6 glioma cells expressed high levels of the inhibitors of differentiation/DNA binding, Id2 and Id4, known repressors of glial cell differentiation. We observed that CpG-rich islands within the Id2 and Id4 genes were bound by PRMT5 and were hypomethylated in PRMT5-deficient cells, suggesting that PRMT5 plays a role in gene silencing during glial cell differentiation. Our findings define a role of PRMT5 in glial cell differentiation and link PRMT5 to epigenetic changes during oligodendrocyte differentiation. PMID:22041901

  17. Hydrology of Polk County, Florida

    USGS Publications Warehouse

    Spechler, Rick M.; Kroening, Sharon E.

    2007-01-01

    Local water managers usually rely on information produced at the State and regional scale to make water-resource management decisions. Current assessments of hydrologic and water-quality conditions in Polk County, Florida, commonly end at the boundaries of two water management districts (South Florida Water Management District and the Southwest Florida Water Management District), which makes it difficult for managers to determine conditions throughout the county. The last comprehensive water-resources assessment of Polk County was published almost 40 years ago. To address the need for current countywide information, the U.S. Geological Survey began a 3?-year study in 2002 to update information about hydrologic and water-quality conditions in Polk County and identify changes that have occurred. Ground-water use in Polk County has decreased substantially since 1965. In 1965, total ground-water withdrawals in the county were about 350 million gallons per day. In 2002, withdrawals totaled about 285 million gallons per day, of which nearly 95 percent was from the Floridan aquifer system. Water-conservation practices mainly related to the phosphate-mining industry as well as the decrease in the number of mines in operation in Polk County have reduced total water use by about 65 million gallons per day since 1965. Polk County is underlain by three principal hydrogeologic units. The uppermost water-bearing unit is the surficial aquifer system, which is unconfined and composed primarily of clastic deposits. The surficial aquifer system is underlain by the intermediate confining unit, which grades into the intermediate aquifer system and consists of up to two water-bearing zones composed of interbedded clastic and carbonate rocks. The lowermost hydrogeologic unit is the Floridan aquifer system. The Floridan aquifer system, a thick sequence of permeable limestone and dolostone, consists of the Upper Floridan aquifer, a middle semiconfining unit, a middle confining unit, and

  18. miR-342 Regulates BRCA1 Expression through Modulation of ID4 in Breast Cancer

    PubMed Central

    Crippa, Elisabetta; Lusa, Lara; De Cecco, Loris; Marchesi, Edoardo; Calin, George Adrian; Radice, Paolo; Manoukian, Siranoush; Peissel, Bernard; Daidone, Maria Grazia

    2014-01-01

    A miRNAs profiling on a group of familial and sporadic breast cancers showed that miRNA-342 was significantly associated with estrogen receptor (ER) levels. To investigate at functional level the role of miR-342 in the pathogenesis of breast cancer, we focused our attention on its “in silico” predicted putative target gene ID4, a transcription factor of the helix-loop-helix protein family whose expression is inversely correlated with that of ER. ID4 is expressed in breast cancer and can negatively regulate BRCA1 expression. Our results showed an inverse correlation between ID4 and miR-342 as well as between ID4 and BRCA1 expression. We functionally validated the interaction between ID4 and miR-342 in a reporter Luciferase system. Based on these findings, we hypothesized that regulation of ID4 mediated by miR-342 could be involved in the pathogenesis of breast cancer by downregulating BRCA1 expression. We functionally demonstrated the interactions between miR-342, ID4 and BRCA1 in a model provided by ER-negative MDA-MB-231 breast cancer cell line that presented high levels of ID4. Overexpression of miR-342 in these cells reduced ID4 and increased BRCA1 expression, supporting a possible role of this mechanism in breast cancer. In the ER-positive MCF7 and in the BRCA1-mutant HCC1937 cell lines miR-342 over-expression only reduced ID4. In the cohort of patients we studied, a correlation between miR-342 and BRCA1 expression was found in the ER-negative cases. As ER-negative cases were mainly BRCA1-mutant, we speculate that the mechanism we demonstrated could be involved in the decreased expression of BRCA1 frequently observed in non BRCA1-mutant breast cancers and could be implicated as a causal factor in part of the familial cases grouped in the heterogeneous class of non BRCA1 or BRCA2-mutant cases (BRCAx). To validate this hypothesis, the study should be extended to a larger cohort of ER-negative cases, including those belonging to the BRCAx class. PMID:24475217

  19. Challenges with using primer IDs to improve accuracy of next generation sequencing.

    PubMed

    Brodin, Johanna; Hedskog, Charlotte; Heddini, Alexander; Benard, Emmanuel; Neher, Richard A; Mild, Mattias; Albert, Jan

    2015-01-01

    Next generation sequencing technologies, like ultra-deep pyrosequencing (UDPS), allows detailed investigation of complex populations, like RNA viruses, but its utility is limited by errors introduced during sample preparation and sequencing. By tagging each individual cDNA molecule with barcodes, referred to as Primer IDs, before PCR and sequencing these errors could theoretically be removed. Here we evaluated the Primer ID methodology on 257,846 UDPS reads generated from a HIV-1 SG3Δenv plasmid clone and plasma samples from three HIV-infected patients. The Primer ID consisted of 11 randomized nucleotides, 4,194,304 combinations, in the primer for cDNA synthesis that introduced a unique sequence tag into each cDNA molecule. Consensus template sequences were constructed for reads with Primer IDs that were observed three or more times. Despite high numbers of input template molecules, the number of consensus template sequences was low. With 10,000 input molecules for the clone as few as 97 consensus template sequences were obtained due to highly skewed frequency of resampling. Furthermore, the number of sequenced templates was overestimated due to PCR errors in the Primer IDs. Finally, some consensus template sequences were erroneous due to hotspots for UDPS errors. The Primer ID methodology has the potential to provide highly accurate deep sequencing. However, it is important to be aware that there are remaining challenges with the methodology. In particular it is important to find ways to obtain a more even frequency of resampling of template molecules as well as to identify and remove artefactual consensus template sequences that have been generated by PCR errors in the Primer IDs.

  20. PTK7 regulates Id1 expression in CD44-high glioma cells

    PubMed Central

    Liu, Qing; Zhang, Chi; Yuan, Jian; Fu, Jun; Wu, Minghua; Su, Jun; Wang, Xiangyu; Yuan, Xianrui; Jiang, Weixi

    2015-01-01

    Background CD44 is a molecular marker associated with molecular subtype and treatment resistance in glioma. More effective therapies will result from approaches aimed at targeting the CD44-high gliomas. Methods Protein tyrosine kinase 7 (PTK7) mRNA expression was analyzed based on The Cancer Genome Atlas glioblastoma dataset. PTK7 expression was depleted through lentivirus-mediated short hairpin RNA knockdown. Terminal deoxynucleotidyl transferase dUTP nick-end labeling was used to evaluate cell apoptosis following PTK7 knockdown. Gene expression analysis was performed on Affymetrix microarray. A nude mice orthotopic tumor model was used to evaluate the in vivo effect of PTK7 depletion. Results PTK7 is highly expressed in CD44-high glioblastoma and predicts unfavorable prognosis. PTK7 knockdown attenuated cell proliferation, impaired tumorigenic potential, and induced apoptosis in CD44-high glioma cell lines. Gene expression analysis identified inhibitor of DNA Binding 1 (Id1) gene as a potential downstream effector for PTK7. Overexpression of Id1 mostly restored the cell proliferation and colony formation attenuated by PTK7 depletion. PTK7 enhanced anchorage-independent growth in normal human astrocytes, which was attenuated by Id1 knockdown. Furthermore, PTK7 regulated Id1 expression through modulating TGF-β/Smad signaling, while pharmacological inhibition on TGF-β/Smad signaling or PTK7/Id1 depletion attenuated TGF-β–stimulated cell proliferation. PTK7 depletion consistently reduced Id1 expression, suppressed tumor growth, and induced apoptosis in a murine orthotopic tumor model, which could be translated into prolonged survival in tumor-bearing mice. Conclusions PTK7 regulates Id1 expression in CD44-high glioma cell lines. Targeting PTK7 could be an effective strategy for treating glioma with high CD44 expression. PMID:25204555

  1. Inhibitor of DNA binding 1 (Id1) induces differentiation and proliferation of mouse embryonic carcinoma P19CL6 cells

    SciTech Connect

    Meng, Qingzhen; Jia, Zhuqing; Wang, Weiping; Li, Binhong; Ma, Kangtao; Zhou, Chunyan

    2011-08-26

    Highlights: {yields} Id1 was upregulated during the cardiac differentiation process of P19CL6 cells. {yields} Id1 upregulated expression of cardiac specific genes Gata4, {alpha}-MHC and ISL1. {yields} Id1 promoted proliferation of P19CL6 cells. {yields} Overexpression of Id1 increased activity of TOP flash. {yields} Wnt3a or LiCl treatment promoted Id1 expression in P19CL6 cells. -- Abstract: The inhibitor of DNA binding (Id) family of genes encodes negative regulators of basic helix-loop-helix transcription factors and has been implicated in such diverse cellular processes as differentiation, proliferation, apoptosis and migration. Id knockout mouse embryos display multiple cardiac defects but the specific role of Id1 in cardiac differentiation is unclear. In the present study, we investigated the function of Id1 in DMSO-induced P19CL6 cells, a widely-accepted cell model of cardiac differentiation. We found that Id1 was upregulated during the cardiac differentiation of P19CL6 cells. The expression of cardiac specific marker genes, Gata4, {alpha}-MHC and ISL1, was upregulated in P19CL6 cells stably transfected with Id1 (P19CL6-Id1) during cardiac differentiation. The overexpression of Id1 reduced the number of cells in G1 phase and increased the cell population in G2, M and S phases, while knockdown of Id1 increased the number of cells in G1 phase from 48.6 {+-} 2.51% to 62.2 {+-} 1.52% at day 0 of cardiac induction, and from 52.5 {+-} 3.41% to 63.7 {+-} 1.02% at day 3 after cardiac induction, indicating that Id1 promoted proliferation of P19CL6 cells. Luciferase assays showed that the activity of TOP flash was higher in P19CL6-Id1 cells than wildtype P19CL6 cells, while Id1 expression was also upregulated in P19CL6 cells treated with Wnt3a or LiCl. This indicates that there may be positive feedback between Id1 and Wnt signaling which plays an important role in cardiac differentiation.

  2. 32 CFR 1602.10 - County.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false County. 1602.10 Section 1602.10 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM DEFINITIONS § 1602.10 County. The word county includes, where applicable, counties, independent cities, and similar...

  3. 32 CFR 1602.10 - County.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false County. 1602.10 Section 1602.10 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM DEFINITIONS § 1602.10 County. The word county includes, where applicable, counties, independent cities, and similar...

  4. 32 CFR 1602.10 - County.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false County. 1602.10 Section 1602.10 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM DEFINITIONS § 1602.10 County. The word county includes, where applicable, counties, independent cities, and similar...

  5. 32 CFR 1602.10 - County.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false County. 1602.10 Section 1602.10 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM DEFINITIONS § 1602.10 County. The word county includes, where applicable, counties, independent cities, and similar...

  6. 32 CFR 1602.10 - County.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false County. 1602.10 Section 1602.10 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM DEFINITIONS § 1602.10 County. The word county includes, where applicable, counties, independent cities, and similar...

  7. Id-1 promotes osteosarcoma cell growth and inhibits cell apoptosis via PI3K/AKT signaling pathway.

    PubMed

    Hao, Liang; Liao, Qi; Tang, Qiang; Deng, Huan; Chen, Lu

    2016-02-12

    Accumulating evidence reveals that Id-1 is upregulated and functions as a potential tumor promoter in several human cancer types. However, the role of Id-1 in osteosarcoma (OS) is unknown. In present study, we found that Id-1 expression was elevated in OS tissues than adjacent normal bone tissues. More importantly, we demonstrated that overexpression of Id-1 is significantly correlated with tumor progression and poor survival in OS patients. Furthermore, increased expression of Id-1 was observed in OS cell lines and ectopic expression of Id-1 significantly enhanced in vitro cell proliferation and promoted in vivo tumor growth, whereas knockdown of Id-1 suppressed OS cells growth. Moreover, our experimental data revealed that Id-1 promotes cell proliferation by facilitating cell cycle progression and inhibits cell apoptosis. Mechanistically, the effects of Id-1 in OS cells is at least partly through activation of PI3K/Akt signaling pathway. Therefore, we identified a tumorigenic role of Id-1 in OS and suggested a potential therapeutic target for OS patients.

  8. Dynamic programming for single nucleotide polymorphism ID identification in systematic association studies.

    PubMed

    Yang, Cheng-Hong; Chuang, Li-Yeh; Cheng, Yu-Huei; Wen, Cheng-Hao; Chang, Hsueh-Wei

    2009-04-01

    Single nucleotide polymorphisms (SNPs) play an important role in personalized medicine. However, the SNP data reported in many association studies provide only the SNP nucleotide/amino acid position, without providing the SNP ID recorded in National Center for Biotechnology Information databases. A tool with the ability to provide SNP ID identification, with a user-friendly interface, is needed. In this paper, a dynamic programming algorithm was used to compare homologs when the processed input sequence is aligned with the SNP FASTA database. Our novel system provides a web-based tool that uses the National Center for Biotechnology Information dbSNP database, which provides SNP sequence identification and SNP FASTA formats. Freely selectable sequence formats for alignment can be used, including general sequence formats (ACGT, [dNTP1/dNTP2] or IUPAC formats) and orientation with bidirectional sequence matching. In contrast to the National Center for Biotechnology Information SNP-BLAST, the proposed system always provides the correct targeted SNP ID (SNP hit), as well as nearby SNPs (flanking hits), arranged in their chromosomal order and contig positions. The system also solves problems inherent in SNP-BLAST, which cannot always provide the correct SNP ID for a given input sequence. Therefore, this system constitutes a novel application which uses dynamic programming to identify SNP IDs from the literature and keyed-in sequences for systematic association studies. It is freely available at http://bio.kuas.edu.tw/SNPosition/.

  9. A Comprehensive Analysis of Primer IDs to Study Heterogeneous HIV-1 Populations.

    PubMed

    Seifert, David; Di Giallonardo, Francesca; Töpfer, Armin; Singer, Jochen; Schmutz, Stefan; Günthard, Huldrych F; Beerenwinkel, Niko; Metzner, Karin J

    2016-01-16

    Determining the composition of viral populations is becoming increasingly important in the field of medical virology. While recently developed computational tools for viral haplotype analysis allow for correcting sequencing errors, they do not always allow for the removal of errors occurring in the upstream experimental protocol, such as PCR errors. Primer IDs (pIDs) are one method to address this problem by harnessing redundant template resampling for error correction. By using a reference mixture of five HIV-1 strains, we show how pIDs can be useful for estimating key experimental parameters, such as the substitution rate of the PCR process and the reverse transcription (RT) error rate. In addition, we introduce a hidden Markov model for determining the recombination rate of the RT PCR process. We found no strong sequence-specific bias in pID abundances (the same RT efficiencies as compared to commonly used short, specific RT primers) and no effects of pIDs on the estimated distribution of the references viruses.

  10. Usefulness of Candida ID2 agar for the presumptive identification of Candida dubliniensis.

    PubMed

    Eraso, Elena; Sahand, Ismail H; Villar-Vidal, María; Marcos, Cristina; Dolores Moragues, María; Madariaga, Lucila; Pontón, José; Quindós, Guillermo

    2006-11-01

    CHROMagar Candida and Candida ID2 are widely used for the isolation and presumptive identification of Candida spp. based on the color of the colonies on these two media. We have studied the usefulness of these chromogenic media for differentiating Candida dubliniensis from Candida albicans isolates. One hundred isolates of C. dubliniensis and 100 C. albicans isolates were tested on Candida ID2, CHROMagar Candida (CHROMagar), and CHROMagar Candida reformulated by BBL. CHROMagar Candida and CHROMagar Candida BBL did not allow a clear differentiation of the two species based upon the shade of the green color of C. dubliniensis colonies. However, on Candida ID2, all C. dubliniensis isolates produced turquoise blue colonies whereas 91% of C. albicans colonies were cobalt blue. The sensitivity and the specificity for differentiating between C. dubliniensis fromC. albicans on Candida ID2 were 100% and 91%, respectively; whereas on CHROMagar Candida these values were 63% and 89% and on CHROMagar Candida BBL they were 18% and 98%. Candida ID2 agar provides a simple and accurate laboratory approach for the identification and differentiation of C. dubliniensis on the basis of the colony color.

  11. Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.

    PubMed

    Kim, Dae In; Jensen, Samuel C; Roux, Kyle J

    2016-01-01

    The nuclear envelope (NE) is a critical cellular structure whose constituents and roles in a myriad of cellular processes seem ever expanding. To determine the underlying mechanisms by which the NE constituents participate in various cellular events, it is necessary to understand the nature of their protein-protein associations. BioID (proximity-dependent biotin identification) is a recently established method to generate a history of protein-protein associations as they occur over time in living cells. BioID is based on fusion of a bait protein to a promiscuous biotin ligase. Expression of the BioID fusion protein in a relevant cellular environment enables biotinylation of vicinal and interacting proteins of the bait protein, permitting isolation and identification by conventional biotin-affinity capture and mass-spec analysis. In this way, BioID provides unique capabilities to identify protein-protein associations at the NE. In this chapter we provide a detailed protocol for the application of BioID to the study of NE proteins.

  12. Meet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID

    PubMed Central

    Varnaitė, Renata

    2016-01-01

    Proximity‐dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a protein of interest in living cells. BioID relies on fusion of the protein of interest with a mutant form of the biotin ligase enzyme BirA (BirA*) that is capable of promiscuously biotinylating proximal proteins irrespective of whether these interact directly or indirectly with the fusion protein or are merely located in the same subcellular neighborhood. The covalent addition of biotin allows the labeled proteins to be purified from cell extracts on the basis of their affinity for streptavidin and identified by mass spectrometry. To date, BioID has been successfully applied to study a variety of proteins and processes in mammalian cells and unicellular eukaryotes and has been shown to be particularly suited to the study of insoluble or inaccessible cellular structures and for detecting weak or transient protein associations. Here, we provide an introduction to BioID, together with a detailed summary of where and how the method has been applied to date, and briefly discuss technical aspects involved in the planning and execution of a BioID study. PMID:27329485

  13. The use of the Kitt Peak IDS in determining galaxy redshifts - Results for five clusters

    NASA Astrophysics Data System (ADS)

    Hintzen, P.

    1980-06-01

    The redshifts of 71 galaxies in five clusters determined using the Kitt Peak Image Dissector Scanner (IDS) are presented and the accuracy of velocities determined with the IDS system is discussed. Data were obtained from observations of absorption or emission lines in the range 3600-5400 A using the IDS on a 2.1-m telescope and effective apertures of 3.8 x 10.3 arcsec from galaxies belonging to the AWM 7 = NGC 1129, A 1213, A 1795, A 2589 and A 2666 clusters. Wavelength errors due to mechanical flexure, off-center guiding and spectrum noise are estimated to provide an uncertainty of + or - 1.21 A or 92 km/sec in measurements of a given spectrum, and comparison of the velocities determined by the Kitt Peak IDS with those determined by the Lick IDS supports the error value. Implications of the mean heliocentric redshifts and relativistically correct velocity dispersions obtained for the structure and membership of the AWM 7 cluster are discussed.

  14. Attitudes of Israeli Teachers and Paraprofessionals towards the New Definition of ID and Their Willingness to Cope with Special Education Law Changes

    ERIC Educational Resources Information Center

    Lifshitz, Hefziba; Nissim, Shoshanna; Cohen, Sara

    2008-01-01

    Attitudes of teachers (N = 96) and paraprofessionals (N = 48) working with students with intellectual disability (ID) towards the new definition of ID (Luckasson et al., 1992, 2002), was examined. The main innovation of the new definition of ID is the cancellation of the four ID levels, suggesting an optimistic view, especially towards individuals…

  15. Energy Improvements in Barnstable County

    SciTech Connect

    2003-09-01

    A fact sheet that describes Barnstable County, Massachusetts, effort to improve energy efficiency through a public appliance trade-in program, installation of compact fluorescent light bulbs in public buildings, and public energy education.

  16. Geothermal development plan: Yuma county

    SciTech Connect

    White, D.H.

    1981-01-01

    One hot spring and 33 wells drilled in the county discharge water at temperatures sufficient for direct-use geothermal applications such as process heat and space heating and cooling. Currently, one industry within the county has been identified which may be able to use geothermal energy for its process heat requirements. Also, a computer simulation model was used to predict geothermal energy on line as a function of time under both private and city-owned utility development of the resource.

  17. Geothermal Development Plan: Pima County

    SciTech Connect

    White, D.H.

    1981-01-01

    Pima County is located entirely within the Basin and Range physiographic province in which geothermal resources are known to occur. Continued growth as indicated by such factors as population growth, employment and income will require large amounts of energy. It is believed that geothermal energy could provide some of the energy that will be needed. Potential users of geothermal energy within the county are identified.

  18. E Proteins and ID Proteins: Helix-Loop-Helix Partners in Development and Disease.

    PubMed

    Wang, Lan-Hsin; Baker, Nicholas E

    2015-11-01

    The basic Helix-Loop-Helix (bHLH) proteins represent a well-known class of transcriptional regulators. Many bHLH proteins act as heterodimers with members of a class of ubiquitous partners, the E proteins. A widely expressed class of inhibitory heterodimer partners-the Inhibitor of DNA-binding (ID) proteins-also exists. Genetic and molecular analyses in humans and in knockout mice implicate E proteins and ID proteins in a wide variety of diseases, belying the notion that they are non-specific partner proteins. Here, we explore relationships of E proteins and ID proteins to a variety of disease processes and highlight gaps in knowledge of disease mechanisms.

  19. Comments on an ID-Based Authenticated Group Key Agreement Protocol with Withstanding Insider Attacks

    NASA Astrophysics Data System (ADS)

    Wu, Tsu-Yang; Tseng, Yuh-Min

    In PKC 2004, Choi et al. proposed an ID-based authenticated group key agreement (AGKA) protocol using bilinear pairings. Unfortunately, their protocol suffered from an impersonation attack and an insider colluding attack. In 2008, Choi et al. presented an improvement to resist insider attacks. In their modified protocol, they used an ID-based signature scheme on transcripts for binding them in a session to prevent replay of transcripts. In particular, they smartly used the batch verification technique to reduce the computational cost. In this paper, we first show that Choi et al.'s modified AGKA protocol still suffers from an insider colluding attack. Then, we prove that the batch verification of the adopted ID-based signature scheme in their modified protocol suffers from a forgery attack.

  20. Eosinophilic cellulitis and panniculitis with generalized vesicular pustular id reaction after a molten aluminum burn.

    PubMed

    Chao, Sheau-Chiou; Lee, Yi-Pei; Lee, Julia Yu-Yun

    2010-01-01

    Hypersensitivity reactions to aluminum are rare. Sensitization has been reported during the continuous application of aluminum-containing antiperspirants or after the injection of aluminum-adsorbed vaccines and allergen extracts containing aluminum. We report a rare case of a nonhealing ulcer at the site of an accidental molten aluminum burn that showed changes of eosinophilic cellulitis and panniculitis histologically. This process was followed by a generalized vesicular and pustular id reaction. The reactions resolved after 1 month of systemic corticosteroid therapy. Two months later, there was a relapse with bullae over the burn scar, accompanied by a pruritic oozing rash on the ears and face; the lesions were controlled again with systemic corticosteroid treatment. The clinicopathologic features suggest that the eosinophilic cellulitis with panniculitis and id reaction in the present case represents an unusual allergic reaction to aluminum. Aluminum allergy may be considered as a possible underlying process in the setting of eosinophilic cellulitis with panniculitis and id reaction.

  1. You can be a wizard of IDS. Is your practice ready to form an integrated delivery system?

    PubMed

    Fabrizio, Nick A

    2007-09-01

    To combat declining reimbursement and strengthen their positions in a highly competitive provider environment, leaders of large medical groups may consider forming an integrate delivery system (IDS) to gain a business advantage. Managing the array of organizational, operational, financial and human relationships affected by an IDS requires careful planning, good timing and a bit of luck. This article, the first of a two-part series, examines what questions physician practice leaders should ask--and answer--before formiing an IDS.

  2. ID-Based Blind Signature and Proxy Blind Signature without Trusted PKG

    NASA Astrophysics Data System (ADS)

    Yu, Yihua; Zheng, Shihui; Yang, Yixian

    Private key escrow is an inherent disadvantage for ID-based cryptosystem, i.e., the PKG knows each signer's private key and can forge the signature of any signer. Blind signature plays a central role in electronic cash system. Private key escrow is more severe in electronic cash system since money is directly involved. To avoid the key escrow problem, we propose an ID-based blind signature and proxy blind signature without trusted PKG. If the dishonest PKG impersonates an honest signer to sign a document, the signer can provide a proof to convince that the PKG is dishonest.

  3. Evaluation of the Mast ID and API 50CH systems for identification of Listeria spp.

    PubMed Central

    Kerr, K G; Rotowa, N A; Hawkey, P M; Lacey, R W

    1990-01-01

    A multipoint inoculation technique (Mast ID) for the identification and species determination of Listeria monocytogenes (sensu strictu) and six other species of the genus Listeria was evaluated. This was compared with the commercially available API 50CH system. Both methods successfully identified all 123 strains tested. The Mast ID system is inexpensive and utilizing a multipoint inoculation technique permits the screening of up to 21 isolates per 9-cm petri dish. The API 50CH system was more expensive and time consuming and is therefore suitable only for the examination of smaller numbers of strains. PMID:2180369

  4. Differential expression of id genes and their potential regulator znf238 in zebrafish adult neural progenitor cells and neurons suggests distinct functions in adult neurogenesis.

    PubMed

    Diotel, Nicolas; Beil, Tanja; Strähle, Uwe; Rastegar, Sepand

    2015-01-01

    Teleost fish display a remarkable ability to generate new neurons and to repair brain lesions during adulthood. They are, therefore, a very popular model to investigate the molecular mechanisms of constitutive and induced neurogenesis in adult vertebrates. In this study, we investigated the expression patterns of inhibitor of DNA binding (id) genes and of their potential transcriptional repressor, znf238, in the whole brain of adult zebrafish. We show that while id1 is exclusively expressed in ventricular cells in the whole brain, id2a, id3 and id4 genes are expressed in broader areas. Interestingly, znf238 was also detected in these regions, its expression overlapping with id2a, id3 and id4 expression. Further detailed characterization of the id-expressing cells demonstrated that (a) id1 is expressed in type 1 and type 2 neural progenitors as previously published, (b) id2a in type 1, 2 and 3 neural progenitors, (c) id3 in type 3 neural progenitors and (d) id4 in postmitotic neurons. Our data provide a detailed map of id and znf238 expression in the brain of adult zebrafish, supplying a framework for studies of id genes function during adult neurogenesis and brain regeneration in the zebrafish.

  5. Somerset County Flood Information System

    USGS Publications Warehouse

    Hoppe, Heidi L.

    2007-01-01

    The timely warning of a flood is crucial to the protection of lives and property. One has only to recall the floods of August 2, 1973, September 16 and 17, 1999, and April 16, 2007, in Somerset County, New Jersey, in which lives were lost and major property damage occurred, to realize how costly, especially in terms of human life, an unexpected flood can be. Accurate forecasts and warnings cannot be made, however, without detailed information about precipitation and streamflow in the drainage basin. Since the mid 1960's, the National Weather Service (NWS) has been able to forecast flooding on larger streams in Somerset County, such as the Raritan and Millstone Rivers. Flooding on smaller streams in urban areas was more difficult to predict. In response to this problem the NWS, in cooperation with the Green Brook Flood Control Commission, installed a precipitation gage in North Plainfield, and two flash-flood alarms, one on Green Brook at Seeley Mills and one on Stony Brook at Watchung, in the early 1970's. In 1978, New Jersey's first countywide flood-warning system was installed by the U.S. Geological Survey (USGS) in Somerset County. This system consisted of a network of eight stage and discharge gages equipped with precipitation gages linked by telephone telemetry and eight auxiliary precipitation gages. The gages were installed throughout the county to collect precipitation and runoff data that could be used to improve flood-monitoring capabilities and flood-frequency estimates. Recognizing the need for more detailed hydrologic information for Somerset County, the USGS, in cooperation with Somerset County, designed and installed the Somerset County Flood Information System (SCFIS) in 1990. This system is part of a statewide network of stream gages, precipitation gages, weather stations, and tide gages that collect data in real time. The data provided by the SCFIS improve the flood forecasting ability of the NWS and aid Somerset County and municipal agencies in

  6. Redox-sensitive vascular smooth muscle cell proliferation is mediated by GKLF and Id3 in vitro and in vivo.

    PubMed

    Nickenig, Georg; Baudler, Stephanie; Müller, Cornelius; Werner, Christian; Werner, Nikos; Welzel, Hilke; Strehlow, Kerstin; Böhm, Michael

    2002-07-01

    Reactive oxygen species such as superoxide and hydroxyl radicals have been implicated in the pathogenic growth of various cell types. The molecular mechanisms involved in redox-sensitive cell growth control are poorly understood. Stimulation of cultured vascular smooth muscle cells (VSMC) with xanthin/xanthin oxidase (X/XO) increases proliferation, whereas stimulation with hydrogen peroxide and Fe3+NTA (H-Fe) causes growth arrest of VSMC. Differential Display led to the identification of two novel, differentially regulated redox-sensitive genes. The dominant negative helix-loop-helix protein Id3 is induced by X/XO and down-regulated by H-Fe. The transcription factor gut-enriched Kruppel-like factor (GKLF) is induced by H-Fe but not by X/XO. Induction of GKLF and inhibition of Id3 via transfection experiments leads to growth arrest, whereas overexpression of Id3 and inhibition of GKLF cause cell growth. Id3 down-regulation is induced via binding of GKLF to the Id3 promotor and concomitantly reduced Id3 gene transcription rate. GKLF induction by H-Fe is mediated through hydroxyl radicals, p38MAP kinase-, calcium-, and protein synthesis-dependent pathways. Id3 is induced by X/XO via superoxide, calcium, p38, and p42/44 MAP kinase. GKLF induces and Id3 depresses expression of p21WAF1/Cip1, p27KIP1, p53. Induction of Id3 is accomplished by angiotensin II via superoxide release. A vascular injury mouse model revealed that Id3 is overexpressed in proliferating vascular tissue in vivo. These findings reveal novel mechanisms of redox-controlled cellular proliferation involving GKLF and Id3 that may have general implications for our understanding of vascular and nonvascular growth control.

  7. Geothermal development plan: Yuma County

    SciTech Connect

    White, D.H.; Goldstone, L.A.

    1982-08-01

    The Yuma County Area Development Plan evaluated the county-wide market potential for utilizing geothermal energy. The study identified four potential geothermal resource areas with temperatures less than 90/sup 0/C (194/sup 0/F), and in addition, two areas are inferred to contain geothermal resources with intermediate (90/sup 0/C to 150/sup 0/C, 194/sup 0/F to 300/sup 0/F) temperature potential. The resource areas are isolated, although one resource area is located near Yuma, Arizona. One resource site is inferred to contain a hot dry rock resource. Anticipated population growth in the county is expected to be 2 percent per year over the next 40 years. The primary employment sector is agriculture, though some light industry is located in the county. Water supplies are found to be adequate to support future growth without advese affect on agriculture. Six firms were found in Yuma County which may be able to utilize geothermal energy for process heat needs. In addition, several agricultural processors were found, concentrated in citrus processing and livestock raising. Geothermal energy utilization projections suggest that by the year 2000, geothermal energy may economically provide the energy equivalent of 53,000 barrels of oil per year to the industrial sector if developed privately. Geothermal utilization projections increase to 132,000 barrels of oil per year by 2000 if a municipal utility developed the resource.

  8. A Provably Secure Revocable ID-Based Authenticated Group Key Exchange Protocol with Identifying Malicious Participants

    PubMed Central

    Tsai, Tung-Tso

    2014-01-01

    The existence of malicious participants is a major threat for authenticated group key exchange (AGKE) protocols. Typically, there are two detecting ways (passive and active) to resist malicious participants in AGKE protocols. In 2012, the revocable identity- (ID-) based public key system (R-IDPKS) was proposed to solve the revocation problem in the ID-based public key system (IDPKS). Afterwards, based on the R-IDPKS, Wu et al. proposed a revocable ID-based AGKE (RID-AGKE) protocol, which adopted a passive detecting way to resist malicious participants. However, it needs three rounds and cannot identify malicious participants. In this paper, we fuse a noninteractive confirmed computation technique to propose the first two-round RID-AGKE protocol with identifying malicious participants, which is an active detecting way. We demonstrate that our protocol is a provably secure AGKE protocol with forward secrecy and can identify malicious participants. When compared with the recently proposed ID/RID-AGKE protocols, our protocol possesses better performance and more robust security properties. PMID:24991641

  9. Support and Self-Efficacy among Latino and White Parents of Children with ID

    ERIC Educational Resources Information Center

    Cohen, Shana R.; Holloway, Susan D.; Dominguez-Pareto, Irenka; Kuppermann, Miriam

    2015-01-01

    Research indicates that mothers of children with ID who receive familial support experience less stress than those who receive less support. Less is known about the relation of support to mothers' evaluation of parenting self-efficacy, particularly in Latino families. We examined the relationship of different types of family support to life…

  10. OpenID connect as a security service in Cloud-based diagnostic imaging systems

    NASA Astrophysics Data System (ADS)

    Ma, Weina; Sartipi, Kamran; Sharghi, Hassan; Koff, David; Bak, Peter

    2015-03-01

    The evolution of cloud computing is driving the next generation of diagnostic imaging (DI) systems. Cloud-based DI systems are able to deliver better services to patients without constraining to their own physical facilities. However, privacy and security concerns have been consistently regarded as the major obstacle for adoption of cloud computing by healthcare domains. Furthermore, traditional computing models and interfaces employed by DI systems are not ready for accessing diagnostic images through mobile devices. RESTful is an ideal technology for provisioning both mobile services and cloud computing. OpenID Connect, combining OpenID and OAuth together, is an emerging REST-based federated identity solution. It is one of the most perspective open standards to potentially become the de-facto standard for securing cloud computing and mobile applications, which has ever been regarded as "Kerberos of Cloud". We introduce OpenID Connect as an identity and authentication service in cloud-based DI systems and propose enhancements that allow for incorporating this technology within distributed enterprise environment. The objective of this study is to offer solutions for secure radiology image sharing among DI-r (Diagnostic Imaging Repository) and heterogeneous PACS (Picture Archiving and Communication Systems) as well as mobile clients in the cloud ecosystem. Through using OpenID Connect as an open-source identity and authentication service, deploying DI-r and PACS to private or community clouds should obtain equivalent security level to traditional computing model.

  11. The Critical Role of the ID in Interactive Television: The Value of Immediacy.

    ERIC Educational Resources Information Center

    Murphy, Karen L.; Farr, Charlotte W.

    The instructional designer (ID) plays a critical role in the development and viability of each course taught at a distance, particularly in the incorporation of immediacy behaviors, or communication behaviors that convey approachability and communicate interpersonal warmth and closeness. Faculty development must be fostered to encourage immediacy…

  12. Single mimivirus particles intercepted and imaged with an X-ray laser (CXIDB ID 1)

    DOE Data Explorer

    Seibert, M. Marvin; Ekeberg, Tomas; Maia, Filipe R.N.C.

    2011-02-02

    These are the files used to reconstruct the images in the paper "Single Mimivirus particles intercepted and imaged with an X-ray laser". Besides the diffracted intensities, the Hawk configuration files used for the reconstructions are also provided. The files from CXIDB ID 1 are the pattern and configuration files for the pattern showed in Figure 2a in the paper.

  13. Single mimivirus particles intercepted and imaged with an X-ray laser (CXIDB ID 2)

    DOE Data Explorer

    Seibert, M. Marvin; Ekeberg, Tomas

    2011-02-02

    These are the files used to reconstruct the images in the paper "Single Mimivirus particles intercepted and imaged with an X-ray laser". Besides the diffracted intensities, the Hawk configuration files used for the reconstructions are also provided. The files from CXIDB ID 2 are the pattern and configuration files for the pattern showed in Figure 2b in the paper.

  14. Improving the detection of chronic migraine: Development and validation of Identify Chronic Migraine (ID-CM)

    PubMed Central

    Serrano, Daniel; Buse, Dawn C; Pavlovic, Jelena M; Blumenfeld, Andrew M; Dodick, David W; Aurora, Sheena K; Becker, Werner J; Diener, Hans-Christoph; Wang, Shuu-Jiun; Vincent, Maurice B; Hindiyeh, Nada A; Starling, Amaal J; Gillard, Patrick J; Varon, Sepideh F; Reed, Michael L

    2015-01-01

    Background Migraine, particularly chronic migraine (CM), is underdiagnosed and undertreated worldwide. Our objective was to develop and validate a self-administered tool (ID-CM) to identify migraine and CM. Methods ID-CM was developed in four stages. (1) Expert clinicians suggested candidate items from existing instruments and experience (Delphi Panel method). (2) Candidate items were reviewed by people with CM during cognitive debriefing interviews. (3) Items were administered to a Web panel of people with severe headache to assess psychometric properties and refine ID-CM. (4) Classification accuracy was assessed using an ICHD-3β gold-standard clinician diagnosis. Results Stages 1 and 2 identified 20 items selected for psychometric validation in stage 3 (n = 1562). The 12 psychometrically robust items from stage 3 underwent validity testing in stage 4. A scoring algorithm applied to four symptom items (moderate/severe pain intensity, photophobia, phonophobia, nausea) accurately classified most migraine cases among 111 people (sensitivity = 83.5%, specificity = 88.5%). Augmenting this algorithm with eight items assessing headache frequency, disability, medication use, and planning disruption correctly classified most CM cases (sensitivity = 80.6%, specificity = 88.6%). Discussion ID-CM is a simple yet accurate tool that correctly classifies most individuals with migraine and CM. Further testing in other settings will also be valuable. PMID:26002700

  15. 77 FR 56241 - Board Meeting; October 17, 2012; Idaho Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-12

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR WASTE TECHNICAL REVIEW BOARD Board Meeting; October 17, 2012; Idaho Falls, ID The U.S. Nuclear Waste Technical Review Board will meet to discuss DOE work on packaging, transporting, and disposing of SNF and HLW. Pursuant to its authority under section 5051 of Public...

  16. Time-Resolved Research at the Advanced Photon Source Beamline 7-ID

    SciTech Connect

    Dufresne, Eric M.; Adams, Bernhard; Arms, Dohn A.; Chollet, Matthieu; Landahl, Eric C.; Li, Yuelin; Walko, Donald A.; Wang, Jin

    2010-08-02

    The Sector 7 undulator beamline (7-ID) of the Advanced Photon Source (APS) is dedicated to time-resolved x-ray research and is capable of ultrafast measurements on the order of 100 ps. Beamline 7-ID has a laser laboratory featuring a Ti:Sapphire system (average power of 2.5W, pulse duration <50 fs, repetition rate 1-5 kHz) that can be synchronized to the bunch pattern of the storage ring. The laser is deliverable to x-ray enclosures, which contain diffractometers, as well as motorized optical tables for table-top experiments. Beamline 7-ID has a single APS Undulator A and uses a diamond (111) double-crystal monochromator, providing good energy resolution over a range of 6-24 keV. Available optics include Kirkpatrick-Baez (KB) mirrors to microfocus the x-ray beam. A variety of time-resolved diffraction and spectroscopy research is available at 7-ID, with experiments being done in the atomic, molecular, optical, chemistry, and solid state (bulk and surface) fields.

  17. Time-Resolved Research at the Advanced Photon Source Beamline 7-ID

    SciTech Connect

    Dufresne, Eric M.; Adams, Bernhard; Arms, Dohn A.; Chollet, Matthieu; Li, Yuelin; Walko, Donald A.; Wang Jin; Landahl, Eric C.

    2010-06-23

    The Sector 7 undulator beamline (7-ID) of the Advanced Photon Source (APS) is dedicated to time-resolved x-ray research and is capable of ultrafast measurements on the order of 100 ps. Beamline 7-ID has a laser laboratory featuring a Ti:Sapphire system (average power of 2.5 W, pulse duration <50 fs, repetition rate 1-5 kHz) that can be synchronized to the bunch pattern of the storage ring. The laser is deliverable to x-ray enclosures, which contain diffractometers, as well as motorized optical tables for table-top experiments. Beamline 7-ID has a single APS Undulator A and uses a diamond (111) double-crystal monochromator, providing good energy resolution over a range of 6-24 keV. Available optics include Kirkpatrick-Baez (KB) mirrors to microfocus the x-ray beam. A variety of time-resolved diffraction and spectroscopy research is available at 7-ID, with experiments being done in the atomic, molecular, optical, chemistry, and solid state (bulk and surface) fields.

  18. OpenID Connect as a security service in cloud-based medical imaging systems.

    PubMed

    Ma, Weina; Sartipi, Kamran; Sharghigoorabi, Hassan; Koff, David; Bak, Peter

    2016-04-01

    The evolution of cloud computing is driving the next generation of medical imaging systems. However, privacy and security concerns have been consistently regarded as the major obstacles for adoption of cloud computing by healthcare domains. OpenID Connect, combining OpenID and OAuth together, is an emerging representational state transfer-based federated identity solution. It is one of the most adopted open standards to potentially become the de facto standard for securing cloud computing and mobile applications, which is also regarded as "Kerberos of cloud." We introduce OpenID Connect as an authentication and authorization service in cloud-based diagnostic imaging (DI) systems, and propose enhancements that allow for incorporating this technology within distributed enterprise environments. The objective of this study is to offer solutions for secure sharing of medical images among diagnostic imaging repository (DI-r) and heterogeneous picture archiving and communication systems (PACS) as well as Web-based and mobile clients in the cloud ecosystem. The main objective is to use OpenID Connect open-source single sign-on and authorization service and in a user-centric manner, while deploying DI-r and PACS to private or community clouds should provide equivalent security levels to traditional computing model. PMID:27340682

  19. 77 FR 55486 - Agency Information Collection Activities: Importer ID Input Record

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-10

    ... collection techniques or the use of other forms of information technology; and (e) the annual costs burden to... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Importer ID Input... and request for comments; Extension of an existing collection of information. SUMMARY: U.S....

  20. 76 FR 43196 - Implementation of the Truth in Caller ID Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... proposed in the Caller ID Act NPRM, 76 FR 16367, the revised rules also specify that blocking or attempting... manipulating caller identification information with the intent to defraud, cause harm, or wrongfully obtain... information with the intent to defraud, cause harm, or wrongfully obtain anything of value. Synopsis of...

  1. 76 FR 16367 - Implementation of the Truth in Caller ID Act of 2009

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-23

    ... Proceedings, 63 FR 24121 (1998). Electronic Filers: Comments may be filed electronically using the Internet by... ID spoofing done with the intent to defraud, cause harm, or wrongfully obtain anything of value. The... information with the intent to defraud, cause harm, or wrongfully obtain anything of value. The Truth...

  2. What's Going on in Your Professor's Head? Demonstrating the Id, Ego, and Superego

    ERIC Educational Resources Information Center

    Segrist, Dan J.

    2009-01-01

    This article describes an in-class activity designed to demonstrate Freud's structural theory of the psyche, specifically the roles of the id, ego, and superego, as well as the interplay among them. Additionally, the activity visually illustrates Freud's ideas about the levels of consciousness associated with these 3 components. Pre-post quiz…

  3. Developing Technical Expertise in Secondary Technical Schools: The Effect of 4C/ID Learning Environments

    ERIC Educational Resources Information Center

    Sarfo, Frederick K.; Elen, Jan

    2007-01-01

    In this study, the effectiveness of learning environments, developed in line with the specifications of the four components instructional design model (4C/ID model) and the additional effect of ICT for fostering the development of technical expertise in traditional Ghanaian classrooms, was assessed. The study had a one-by-one-by-two…

  4. Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID).

    PubMed

    Schweingruber, Christoph; Soffientini, Paolo; Ruepp, Marc-David; Bachi, Angela; Mühlemann, Oliver

    2016-01-01

    Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-protein interactions. Here, we used BioID, on its own and combined with co-IP, to identify proteins involved in nonsense-mediated mRNA decay (NMD), a post-transcriptional mRNA turnover pathway that targets mRNAs that fail to terminate translation properly. In particular, we expressed BirA* fused to the well characterized NMD factors UPF1, UPF2 and SMG5 and detected by liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) the streptavidin-purified biotinylated proteins. While the identified already known interactors confirmed the usefulness of BioID, we also found new potentially important interactors that have escaped previous detection by co-IP, presumably because they associate only weakly and/or very transiently with the NMD machinery. Our results suggest that SMG5 only transiently contacts the UPF1-UPF2-UPF3 complex and that it provides a physical link to the decapping complex. In addition, BioID revealed among others CRKL and EIF4A2 as putative novel transient interactors with NMD factors, but whether or not they have a function in NMD remains to be elucidated. PMID:26934103

  5. OpenID Connect as a security service in cloud-based medical imaging systems.

    PubMed

    Ma, Weina; Sartipi, Kamran; Sharghigoorabi, Hassan; Koff, David; Bak, Peter

    2016-04-01

    The evolution of cloud computing is driving the next generation of medical imaging systems. However, privacy and security concerns have been consistently regarded as the major obstacles for adoption of cloud computing by healthcare domains. OpenID Connect, combining OpenID and OAuth together, is an emerging representational state transfer-based federated identity solution. It is one of the most adopted open standards to potentially become the de facto standard for securing cloud computing and mobile applications, which is also regarded as "Kerberos of cloud." We introduce OpenID Connect as an authentication and authorization service in cloud-based diagnostic imaging (DI) systems, and propose enhancements that allow for incorporating this technology within distributed enterprise environments. The objective of this study is to offer solutions for secure sharing of medical images among diagnostic imaging repository (DI-r) and heterogeneous picture archiving and communication systems (PACS) as well as Web-based and mobile clients in the cloud ecosystem. The main objective is to use OpenID Connect open-source single sign-on and authorization service and in a user-centric manner, while deploying DI-r and PACS to private or community clouds should provide equivalent security levels to traditional computing model.

  6. 32 CFR 161.7 - ID card life-cycle procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of DoD ID cards. The use of the CAC, as the DoD Federal personal identity verification (PIV) card, is... 201-1, “Personal Identity Verification (PIV) of Federal Employees and Contractors” (available at http... Memorandum, “Final Credentialing Standards for Issuing Personal Identity Verification Cards under...

  7. Use of false ID cards and other deceptive methods to purchase alcoholic beverages during high school.

    PubMed

    Schwartz, R H; Farrow, J A; Banks, B; Giesel, A E

    1998-01-01

    Altered motor vehicle drivers's licenses or other falsified or counterfeit photo identification cards are widely and illegally used by teenagers to obtain beer and other alcohol beverages. We obtained information on the methods currently used by teenagers to purchase beer and wine by asking nine hundred teenagers, between 16-19 years old to complete a brief, confidential questionnaire. High school students most often obtained alcoholic beverages by requesting someone of legal age to purchase it for them. College students used borrowed, altered, or counterfeit identification (ID) more often than high school students. Photo IDs purchased through mail order from a magazine advertisement were used infrequently and when use was attempted, they were sometimes (25%) unsuccessful. Fifteen percent of high school students, 14% of college freshmen, and 24% of teenage drug abusers were able to purchase beer by the case with borrowed, altered, or fake ID. Suggestions to reduce sales of alcohol-containing beverages to minors include universal "carding" of prospective purchasers, use of two view or hologram photos on a drivers' license, requiring three different ID cards at the point of purchase, and penalties to stores that fail to make a good effort to identify underage customers. PMID:9789157

  8. Suppression of mammary epithelial cell differentiation by the helix-loop-helix protein Id-1

    SciTech Connect

    Desprez, P.; Hara, E.; Bissell, M.J.

    1995-06-01

    Cell proliferation and differentiation are precisely coordinated during the development and maturation of the mammary gland, and this balance invariably is disrupted during carcinogenesis. Little is known about the cell-specific transcription factors that regulate these processes in the mammary gland. The mouse mammary epithelial cell line SCp2 grows well under standard culture conditions but arrests growth, forms alveolus-like structures, and expresses {beta}-casein, a differentiation marker, 4 to 5 days after exposure to basement membrane and lactogenic hormones (differentiation signals). The authors show that this differentiation entails a marked decline in the expression of Id-1, a helix-loop-helix (HLH) protein that inactivates basic HLH transcription factors in other cell types. SCp2 cells stably transfected with an Id-1 expression vector grew more rapidly than control cells under standard conditions, but in response to differentiation signals, they lost three-dimensional organization, invaded the basement membrane, and then resumed growth. SCp2 cells expressing an Id-1 antisense vector grew more slowly than controls; in response to differentiation signals, they remained stably growth arrested and fully differentiated, as did control cells. The authors suggest that Id-1 renders cells refractory to differentiation signals and receptive to growth signals by inactivating one or more basic HLH proteins that coordinate growth and differentiation in the mammary epithelium. 53 refs., 6 figs.

  9. 76 FR 28306 - Amendment of Class D and Class E Airspace; Idaho Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ... Federal Register a notice of proposed rulemaking to amend controlled airspace at Idaho Falls, ID (76 FR...'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant... continues to read as follows: Authority: 49 U.S.C. 106(g), 40103, 40113, 40120; E. O. 10854, 24 FR 9565,...

  10. 76 FR 25537 - Amendment of Class E Airspace; McCall, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-05

    ... controlled airspace at McCall, ID (76 FR 8324). Interested parties were invited to participate in this... Executive Order 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR..., 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The...

  11. 75 FR 15741 - Applied Materials; Boise, ID; Notice of Termination of Investigation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Applied Materials; Boise, ID; Notice of Termination of Investigation Pursuant to Section 221 of the Trade Act of 1974, as amended, an investigation was initiated in response...

  12. 76 FR 9266 - Proposed Amendment of Class D and Class E Airspace; Idaho Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ...'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant.... 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by reference in 14 CFR... Falls, ID AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed...

  13. Extraction of words from the national ID cards for automated recognition

    NASA Astrophysics Data System (ADS)

    Akhter, Md. Rezwan; Bhuiyan, Md. Hasanuzzaman; Uddin, Mohammad Shorif

    2011-10-01

    The government of Bangladesh introduced national ID cards in 2008 for all peoples of age 18 years and above. This card is now a de-facto identity document and finds diverse applications in vote casting, bank account opening, telephone subscribing as well as in many real life transactions and security checking. To get real fruits of this versatile ID card, automated retrieving and recognition of an independent person from this extra large national database is an ultimate necessity. This work is the first step to fill this gap in making the recognition in automated fashion. Here we have investigated an image analysis technique to extract the words that will be used in subsequent recognition steps. At first scanned ID card image is used as an input into the computer system and then the target text region is separated from the picture region. The text region is used for separation of lines and words on the basis of the vertical and horizontal projections of image intensity, respectively. Experimentation using real national ID cards confirms the effectiveness of our technique.

  14. 75 FR 11936 - Hewlett Packard; Technical Support Call Center; Boise, ID; Notice of Termination of Investigation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Hewlett Packard; Technical Support Call Center; Boise, ID; Notice of... Packard, Technical Support Call Center, Boise, Idaho. The petitioner has requested that the petition...

  15. Coupling Molecular Modeling to the Traditional "IR-ID" Exercise in the Introductory Organic Chemistry Laboratory

    ERIC Educational Resources Information Center

    Stokes-Huby, Heather; Vitale, Dale E.

    2007-01-01

    This exercise integrates the infrared unknown identification ("IR-ID") experiment common to most organic laboratory syllabi with computer molecular modeling. In this modification students are still required to identify unknown compounds from their IR spectra, but must additionally match some of the absorptions with computed frequencies they…

  16. Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)

    PubMed Central

    Schweingruber, Christoph; Soffientini, Paolo; Ruepp, Marc-David; Bachi, Angela; Mühlemann, Oliver

    2016-01-01

    Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-protein interactions. Here, we used BioID, on its own and combined with co-IP, to identify proteins involved in nonsense-mediated mRNA decay (NMD), a post-transcriptional mRNA turnover pathway that targets mRNAs that fail to terminate translation properly. In particular, we expressed BirA* fused to the well characterized NMD factors UPF1, UPF2 and SMG5 and detected by liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) the streptavidin-purified biotinylated proteins. While the identified already known interactors confirmed the usefulness of BioID, we also found new potentially important interactors that have escaped previous detection by co-IP, presumably because they associate only weakly and/or very transiently with the NMD machinery. Our results suggest that SMG5 only transiently contacts the UPF1-UPF2-UPF3 complex and that it provides a physical link to the decapping complex. In addition, BioID revealed among others CRKL and EIF4A2 as putative novel transient interactors with NMD factors, but whether or not they have a function in NMD remains to be elucidated. PMID:26934103

  17. 75 FR 47515 - Identification (ID) Cards for Members of the Uniformed Services, Their Dependents, and Other...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-06

    ... for common access card (CAC) life-cycle compliance with Federal Information Processing Standards (FIPS...(AT&L), establish policy and oversight for CAC life-cycle compliance with FIPS Publication 201-1. (2... eligibility through DEERS. (2) Develop and implement Component-level ID card life-cycle procedures to...

  18. 78 FR 9029 - Nez Perce-Clearwater National Forests; ID; Clear Creek Integrated Restoration Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-07

    ... Forest Service Nez Perce-Clearwater National Forests; ID; Clear Creek Integrated Restoration Project... Creek Integrated Restoration Draft Environmental Impact Statement (DEIS). The DEIS will include two site... adopt the Regional soils standard for the Clear Creek Integrated Restoration project area. The...

  19. 78 FR 40382 - Modification of Class D and E Airspace; Twin Falls, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-05

    ... Falls Joslin Field-Magic Valley Regional Airport, Twin Falls, ID (78 FR 25406). Interested parties were... Executive Order 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR... read as follows: Authority: 49 U.S.C. 106(g), 40103, 40113, 40120; E. O. 10854, 24 FR 9565, 3 CFR,...

  20. The Id, Ego and Super-Ego in "Pride and Prejudice"

    ERIC Educational Resources Information Center

    Liang, Yamin

    2011-01-01

    This paper mainly analyses the the id, ego, and super-ego which exists in the main character Elizabeth from several aspects, such as her pursuit for love, her prejudice towards Mr. Darcy, and the changes in her attitudes towards Wickham. This analysis helps readers appreciate this masterpiece from a different aspect which is related to the…

  1. National Plant Diagnostic Network, Taxonomic training videos: Introduction to AphID

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Training is a critical part of aphid (Hemiptera: Aphididae) identification. This video provides visual instruction on the use of the expert system, AphID, for aphid examination and identification. The video demonstrates the use of different training modules that allow the user to gain familiarity wi...

  2. 75 FR 22695 - Establishment of Class E Airspace; Bonners Ferry, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... Register a notice of proposed rulemaking to amend controlled airspace at Bonners Ferry, ID (74 FR 58570... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of... continues to read as follows: Authority: 49 U.S.C. 106(g), 40103, 40113, 40120; E.O. 10854, 24 FR 9565,...

  3. An Integral ASIE ID Model: The 21st Century Instructional Design Model for Teachers

    ERIC Educational Resources Information Center

    Zain, Ismail Md.; Muniandy, Balakrishnan; Hashim, Wahid

    2016-01-01

    Design of instruction is an important feature in teacher education at fulfilling the needs of 4Cs ("critical thinker," "communicator," "collaborator," "creator") developing "a globally competitive learners". As Instructional design models (ID) need to move from adopting a standard approach to…

  4. Epigenetic regulation of ID4 in the determination of the BRCAness phenotype in breast cancer.

    PubMed

    Branham, M T; Campoy, E; Laurito, S; Branham, R; Urrutia, G; Orozco, J; Gago, F; Urrutia, R; Roqué, M

    2016-01-01

    BRCAness breast tumors represent a group of sporadic tumors characterized by a reduction in BRCA1 gene expression. As BRCA1 is involved in double-strand breaks (DSBs) repair, dysfunctional BRCA pathway could make a tumor sensitive to DNA damaging drugs (e.g., platinum agents). Thus, accurately identifying BRCAness could contribute to therapeutic decision making in patients harboring these tumors. The purpose of this study was to identify if BRCAness tumors present a characteristic methylation profile and/or were related to specific clinico-pathological features. BRCAness was measured by MLPA in 63 breast tumors; methylation status of 98 CpG sites within 84 cancer-related genes was analyzed by MS-MLPA. Protein and mRNA expressions of the selected genes were measured by quantitative real-time PCR and Western Blot. BRCAness was associated with younger age, higher nuclear pleomorphism, and triple-negative (TN) status. Epigenetically, we found that the strongest predictors for BRCAness tumors were the methylations of MLH1 and PAX5 plus the unmethylations of CCND2 and ID4. We determined that ID4 unmethylation correlated with the expression levels of both its mRNA and protein. We observed an inverse relation between the expressions of ID4 and BRCA1. To the best of our knowledge, this is the first report suggesting an epigenetic regulation of ID4 in BRCAness tumors. Our findings give new information of BRCAness etiology and encourage future studies on potential drug targets for BRCAness breast tumors.

  5. Use of false ID cards and other deceptive methods to purchase alcoholic beverages during high school.

    PubMed

    Schwartz, R H; Farrow, J A; Banks, B; Giesel, A E

    1998-01-01

    Altered motor vehicle drivers's licenses or other falsified or counterfeit photo identification cards are widely and illegally used by teenagers to obtain beer and other alcohol beverages. We obtained information on the methods currently used by teenagers to purchase beer and wine by asking nine hundred teenagers, between 16-19 years old to complete a brief, confidential questionnaire. High school students most often obtained alcoholic beverages by requesting someone of legal age to purchase it for them. College students used borrowed, altered, or counterfeit identification (ID) more often than high school students. Photo IDs purchased through mail order from a magazine advertisement were used infrequently and when use was attempted, they were sometimes (25%) unsuccessful. Fifteen percent of high school students, 14% of college freshmen, and 24% of teenage drug abusers were able to purchase beer by the case with borrowed, altered, or fake ID. Suggestions to reduce sales of alcohol-containing beverages to minors include universal "carding" of prospective purchasers, use of two view or hologram photos on a drivers' license, requiring three different ID cards at the point of purchase, and penalties to stores that fail to make a good effort to identify underage customers.

  6. 17 CFR 239.63 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... has been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password...

  7. 17 CFR 249.446 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... has been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password...

  8. 17 CFR 239.63 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... has been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password...

  9. 17 CFR 269.7 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears in the... been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password Modification...

  10. 17 CFR 269.7 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears in the... been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password Modification...

  11. 17 CFR 249.446 - Form ID, uniform application for access codes to file on EDGAR.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...: For Federal Register citations affecting Form ID, see the List of CFR Sections Affected, which appears... has been authorized by the filer. (c) Password (PW)—allows a filer, filing agent or training agent to log on to the EDGAR system, submit filings, and change its CCC. (d) Password...

  12. 77 FR 50417 - Proposed Amendment of Class D and Class E Airspace; Lewiston, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... your comments. You may also submit comments through the Internet at http://www.regulations.gov . FOR..., ID (77 FR 32921). Also the geographic coordinates of the airport and navigation aids would be... phone number). You may also submit comments through the Internet at...

  13. 78 FR 51677 - Children's Online Privacy Protection Rule Proposed Parental Consent Method; AssertID, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-21

    ... meets the requirements for parental consent described in 16 CFR 312.5(b)(1). \\1\\ 64 FR 59888 (1999). \\2\\ 16 CFR part 312. \\3\\ 78 FR 3972 (2013). \\4\\ 16 CFR 312.12(a); 78 FR at 3991-3992, 4013. Pursuant to... Parental Consent Method; AssertID, Inc. Application for Approval of Parental Consent Method AGENCY:...

  14. 78 FR 61442 - Idaho Disaster #ID-00027 Declaration of Economic Injury

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-03

    ... ADMINISTRATION Idaho Disaster ID-00027 Declaration of Economic Injury AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Economic Injury Disaster Loan (EIDL) declaration for the... the Administrator's EIDL declaration, applications for economic injury disaster loans may be filed...

  15. Certification of Completion of Item 2 of ASC FY07 Level-2 Milestone ID #2380

    SciTech Connect

    Lipari, D A

    2007-03-28

    This report documents the completion of Item 2 of the three milestone deliverables that comprise Milestone ID 2380: Deploy selected Tri-Lab resource manager at LLNL and develop support model. Specifically: LLNL will integrate and support a commercial resource manager software product at LLNL to be used across the tri-lab HPC facilities.

  16. 75 FR 41773 - Proposed Amendment of Class E Airspace; Arco, ID

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does..., 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 2. The incorporation by... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Arco, ID...

  17. [Identification of a novel mutation of IDS gene from a Chinese pedigree with MPS II].

    PubMed

    GUO, Yi-Bin; PAN, Hong-Da; GUO, Chun-Miao; LI, Yong-Mei; CHEN, Lu-Ming

    2009-11-01

    The purpose of this study was to understand the molecular genetic mechanism of mucopolysaccharidosis type II (MPS II) and to provide a prerequisite for future prenatal gene diagnosis. A preliminary diagnosis was made by qualitative detection of Urinary Glycosaminoglycans of the suspected MPS II proband. Then, mutation detection was performed on the proband and his family members with PCR and direct sequencing of PCR products. After the novel mutation of c.876 del 2 in IDS gene was detected, sequence analysis was performed on exon 6 of IDS gene of the 135 cases, which consisted of 120 randomly selected normal controls, and other 15 patients with MPS I, IV, and VI other than MPS II. Besides, the patho-genicity of the novel mutation was analyzed with the following 2 methods: conservative analysis of the sequence of muta-tion spots of different species and the direct test of the IDS enzyme activity of the patient and his relative family members. The result of uroscopy of the proband was strong positive (GAGs +++). There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine (F) glutamine (Q) of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively. These results illustrated that the deletion and frame-shift mutation of c.876-877 del TC detected was a novel pathologic mutation, which was the underlying cause of

  18. Chronic kidney disease in older people with intellectual disability: results of the HA-ID study.

    PubMed

    de Winter, C F; Echteld, M A; Evenhuis, H M

    2014-03-01

    With increasing longevity and cardiovascular events, chronic kidney disease may also become a significant problem in older people with intellectual disability (ID). We studied prevalence and associations of chronic kidney disease as part of the Healthy Ageing and Intellectual Disability (HA-ID) study, a large Dutch cross-sectional study among people with ID aged 50 years and over, using creatinine and cystatin-C measurement in plasma. Glomerular filtration rate (GFR) was calculated using the Modification of Diet in Renal Disease (MDRD) and Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equations. Equations based on creatinine (as the MDRD equation) may underestimate kidney dysfunction in people with sarcopenia, because low muscle mass leads to a low creatinine production. Therefore, also prevalence of chronic kidney disease was studied in the sarcopenic group, using different GFR equations. Prevalence of chronic kidney disease, among 635 participants, was 15.3%, which equals prevalence in the general Dutch population. In the group of participants with sarcopenia (n=82), the CKD-EPI equation based on creatinine and cystatin-C gave a higher prevalence of chronic kidney disease than did the MDRD equation, but confidence intervals were very wide. Chronic kidney disease was associated with higher age, Down syndrome, obesity, hypercholesterolemia and hypothyroid disease. GFR should be measured in all older people with ID and polypharmacy, and in older people with ID and Down syndrome as part of the regular health checks. Moreover, if sarcopenia is present and information on GFR is required, this should not be measured based on creatinine only, but additional measures, such as cystatin-C, should be taken into account.

  19. A 3' splice site mutation of IDS gene in a Chinese family with mucopolysaccharidosis type II.

    PubMed

    Jin, Ping; Hao, Jing-Wen; Chen, Ke; Dong, Chang-Sheng; Yang, You-Bo; Mo, Zhao-Hui

    2013-10-10

    The purpose of this study was to identify the underlying genetic cause in a four generation Chinese family diagnosed with mucopolysaccharidosis type II. Peripheral blood samples were collected from family members and the iduronate-2-sulfatase (IDS) gene was analyzed by DNA sequencing. The impact of IDS mutations on mRNA transcription was determined by quantitative real-time RT-PCR (qRT-PCR) in both patients as well as in healthy control samples. In addition, RT-PCR was performed to confirm the characteristics of a found mutation located in non-canonical splicing site. A 3' splice site mutation c.880-8A>G (IVS 6-8A>G) was identified in two members of the analyzed MPS II family and sequencing of RT-PCR products showed that this mutation activates an upstream cryptic splice-site in intron 6, leads to the 7 nucleotide insertion in exon 7, which in turn results in an exon 7 frameshift introducing a premature stop codon and shorter peptide chain. In addition, qRT-PCR products from the two patients showed a reduced IDS mRNA expression (43.9% and 71.2%, respectively), when compared with the average IDS mRNA expression in healthy control samples, possibly as a result of nonsense-mediated mRNA decay. In conclusion, in this study, we have identified an IDS gene splice mutation which is associated with clinically attenuated MPS II phenotype. In addition, our study accentuates the importance of cDNA analysis in the detection of intronic mutations, since in the studies examining only gDNA, the link between genotype and phenotype may have been misinterpreted.

  20. Geothermal development plan: Maricopa county

    SciTech Connect

    White, D.H.

    1981-01-01

    Maricopa county is the area of Arizona receiving top priority since it contains over half of the state's population. The county is located entirely within the Basin and Range physiographic region in which geothermal resources are known to occur. Several approaches were taken to match potential users to geothermal resources. One approach involved matching some of the largest facilities in the county to nearby geothermal resources. Other approaches involved identifying industrial processes whose heat requirements are less than the average assessed geothermal reservoir temperature of 110/sup 0/C (230/sup 0/F). Since many of the industries are located on or near geothermal resources, geothermal energy potentially could be adapted to many industrial processes.

  1. Archuleta County CO Lineaments

    DOE Data Explorer

    Zehner, Richard E.

    2012-01-01

    Citation Information: Originator: Earth Science &Observation Center (ESOC), CIRES, University of Colorado at Boulder Originator: Geothermal Development Associates, Reno, Nevada Publication Date: 2012 Title: Archuleta Lineaments Edition: First Publication Information: Publication Place: Reno Nevada Publisher: Geothermal Development Associates, Reno, Nevada Description: This layer traces apparent topographic and air-photo lineaments in the area around Pagosa springs in Archuleta County, Colorado. It was made in order to identify possible fault and fracture systems that might be conduits for geothermal fluids. Geothermal fluids commonly utilize fault and fractures in competent rocks as conduits for fluid flow. Geothermal exploration involves finding areas of high near-surface temperature gradients, along with a suitable “plumbing system” that can provide the necessary permeability. Geothermal power plants can sometimes be built where temperature and flow rates are high. To do this, georeferenced topographic maps and aerial photographs were utilized in an existing GIS, using ESRI ArcMap 10.0 software. The USA_Topo_Maps and World_Imagery map layers were chosen from the GIS Server at server.arcgisonline.com, using a UTM Zone 13 NAD27 projection. This line shapefile was then constructed over that which appeared to be through-going structural lineaments in both the aerial photographs and topographic layers, taking care to avoid manmade features such as roads, fence lines, and right-of-ways. These lineaments may be displaced somewhat from their actual location, due to such factors as shadow effects with low sun angles in the aerial photographs. Note: This shape file was constructed as an aid to geothermal exploration in preparation for a site visit for field checking. We make no claims as to the existence of the lineaments, their location, orientation, and nature. Spatial Domain: Extent: Top: 4132831.990103 m Left: 311979.997741 m Right: 331678.289280 m Bottom: 4116067

  2. 78 FR 9446 - Advance Nanotech, Inc., Advanced ID Corp., Aeon Holdings, Inc. (n/k/a BCM Energy Partners, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-08

    ... COMMISSION Advance Nanotech, Inc., Advanced ID Corp., Aeon Holdings, Inc. (n/k/a BCM Energy Partners, Inc..., Ltd., EcoReady Corp., EnDevCo, Inc., Electronic Kourseware International, Inc., Ensign Services, Inc... accurate information concerning the securities of Advanced ID Corp. because it has not filed any...

  3. Performance of Candida ID, a New Chromogenic Medium for Presumptive Identification of Candida Species, in Comparison to CHROMagar Candida

    PubMed Central

    Willinger, Birgit; Hillowoth, Cornelia; Selitsch, Brigitte; Manafi, Mammad

    2001-01-01

    Candida ID agar allows identification of Candida albicans and differentiation of other Candida species. In comparison with CHROMagar Candida, we evaluated the performance of this medium directly from 596 clinical specimens. In particular, detection of C. albicans after 24 h of incubation was easier on Candida ID (sensitivity, 96.8%) than on CHROMagar (sensitivity, 49.6%). PMID:11574621

  4. TGFβ-Id1 Signaling Opposes Twist1 and Promotes Metastatic Colonization Via a Mesenchymal-to-Epithelial Transition

    PubMed Central

    Stankic, Marko; Pavlovic, Svetlana; Chin, Yvette; Brogi, Edi; Padua, David; Norton, Larry; Massague, Joan; Benezra, Robert

    2014-01-01

    SUMMARY ID genes are required for breast cancer colonization of the lungs, but the mechanism remains poorly understood. Here, we show that Id1 expression induces a stem-like phenotype in breast cancer cells, while retaining epithelial properties, contrary to the notion that cancer stem-like properties are inextricably linked to the mesenchymal state. During metastatic colonization, Id1 induces a mesenchymal-to-epithelial transition (MET), specifically in cells whose mesenchymal state is dependent on the Id1 target protein Twist1 but not at the primary site, where this state is controlled by the zinc-finger protein Snail1. Knockdown of Id expression in metastasizing cells prevents MET and dramatically reduces lung colonization. Furthermore, Id1 is induced by TGFβ only in cells that have first undergone EMT, demonstrating that EMT is a pre-requisite for subsequent Id1-induced MET during lung colonization. Collectively, these studies underscore the importance of Id-mediated phenotypic switching during distinct stages of breast cancer metastasis. PMID:24332369

  5. Health Professionals' Attitudes and Emotions towards Working with Adults with Intellectual Disability (ID) and Mental Ill Health

    ERIC Educational Resources Information Center

    Rose, N.; Kent, S.; Rose, J.

    2012-01-01

    Objectives: Mainstream mental health services are providing more care for individuals with an intellectual disability (ID); this has implications for staff and service users. Attitudes of staff towards people with ID in mental health services may be negative and negative staff attitudes may have a detrimental impact on service provision. Design: A…

  6. Limited Concordance between Teachers, Parents and Healthcare Professionals on the Presence of Chronic Diseases in ID-Adolescents

    ERIC Educational Resources Information Center

    Oeseburg, B.; Jansen, D. E. M. C.; Reijneveld, S. A.; Dijkstra, G. J.; Groothoff, J. W.

    2010-01-01

    Evidence on teachers' knowledge about somatic and mental chronic diseases among ID-adolescent compared to the knowledge parents and healthcare professionals have, is limited. The aim of this study is: (1) to assess the knowledge of teachers on the presence of chronic diseases in ID-adolescents; (2) to compare teachers with parents and healthcare…

  7. Id1 expression promotes peripheral CD4{sup +} T cell proliferation and survival upon TCR activation without co-stimulation

    SciTech Connect

    Liu, Chen; Jin, Rong; Wang, Hong-Cheng; Tang, Hui; Liu, Yuan-Feng; Qian, Xiao-Ping; Sun, Xiu-Yuan; Ge, Qing; Sun, Xiao-Hong; Zhang, Yu

    2013-06-21

    Highlights: •Id1 expression enables naïve T cell proliferation without anti-CD28 co-stimulation. •Id1 expression facilitates T cells survival when stimulated with anti-CD3. •Elevation of IL-2 production by Id1 contributes increased proliferation and survival. •Id1 potentiates NF-κB activation by anti-CD3 stimulation. -- Abstract: Although the role of E proteins in the thymocyte development is well documented, much less is known about their function in peripheral T cells. Here we demonstrated that CD4 promoter-driven transgenic expression of Id1, a naturally occurring dominant-negative inhibitor of E proteins, can substitute for the co-stimulatory signal delivered by CD28 to facilitate the proliferation and survival of naïve CD4{sup +} cells upon anti-CD3 stimulation. We next discovered that IL-2 production and NF-κB activity after anti-CD3 stimulation were significantly elevated in Id1-expressing cells, which may be, at least in part, responsible for the augmentation of their proliferation and survival. Taken together, results from this study suggest an important role of E and Id proteins in peripheral T cell activation. The ability of Id proteins to by-pass co-stimulatory signals to enable T cell activation has significant implications in regulating T cell immunity.

  8. Initial Validation of the Chinese Quality of Life Questionnaire-Intellectual Disabilities (CQOL-ID): A Cultural Perspective

    ERIC Educational Resources Information Center

    Wong, P. K. S.; Wong, D. F. K.; Schalock, R. L.; Chou, Y-C.

    2011-01-01

    Background: In the field of intellectual disabilities (ID), the quality of life concept has been developing rapidly in Chinese societies including Hong Kong, mainland China and Taiwan. However, there is a lack of locally validated instruments to measure the quality of life of people with ID. The study reported in this paper attempted to validate…

  9. 76 FR 13172 - Placer County Water Agency

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-10

    ... Energy Regulatory Commission Placer County Water Agency Notice of Application Tendered for Filing with... Filed: February 23, 2011 d. Applicant: Placer County Water Agency e. Name of Project: Middle Fork... Manager, Placer County Water Agency, 144 Ferguson Road, Auburn, CA 95603; Telephone: (530) 823-4490....

  10. Community Analysis for the Pitkin County Library.

    ERIC Educational Resources Information Center

    Kirwin, Florence M.

    This document analyzes the social and demographic characteristics of Pitkin County, Colorado (population 12,000) and the Pitkin County Library located in Aspen (24,000 volumes). The report contains statistics gathered from the 1978 Mid-Term U.S. Bureau Census of Pitkin County, as well as other statistics from local, state, and federal sources; the…

  11. Community Types and Mortality in Georgia Counties

    ERIC Educational Resources Information Center

    Young, Frank W.

    2012-01-01

    Using an "ecological regional analysis" methodology for defining types of communities and their associated mortality rates, this study of Georgia's 159 counties finds that the suburban and town centered counties have low mortality while the city-centered type predicts low mortality for the whites. The military-centered counties do not predict. The…

  12. The surficial aquifer in Pinellas County, Florida

    USGS Publications Warehouse

    Causseaux, K.W.

    1985-01-01

    The surficial aquifer in Pinellas County, Florida, contains potable water throughout most of the county and is a potential source of water to augment the public supply that is presently imported from adjacent counties. The county accounts for 38 percent of the public supply consumption of ground water in the 11-county area of west-central Florida and 68 percent of this water is imported from two adjacent counties. The surficial aquifer has a saturated thickness of more than 30 feet throughout most of the county. Specific capacity per foot of screen for wells is less than 0.1 gallon per minute per foot of drawdown in some parts of the county, but yield is sufficient in most of the county for many small uses with shallow-well pumps. Minimum potential yield varies from 5 gallons per minute in the northern part of the county to more than 30 gallons per minute in the south. Concentrations of iron are high enough in parts of the county to cause staining. Chloride concentrations are less than 100 milligrams per liter in most of the county and do not pose a problem for many uses. (USGS)

  13. Trouble Brewing in Orange County. Policy Brief

    ERIC Educational Resources Information Center

    Buck, Stuart

    2010-01-01

    Orange County will soon face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that Orange County faces a total $41.2 billion liability for retiree benefits that are underfunded--including $9.4 billion for the county pension system and an estimated…

  14. [Comparison of Phoenix™ Yeast ID Panel and API® ID 32C commercial systems for the identification of Candida species isolated from clinical samples].

    PubMed

    Gayibova, Ülkü; Dalyan Cılo, Burcu; Ağca, Harun; Ener, Beyza

    2014-07-01

    Opportunistic fungal pathogens are one of the important causes of nosocomial infections, and several different types of yeasts, especially Candida species are increasingly recovered from immunocompromised patients. Since many of the yeasts are resistant to the commonly used antifungal agents, the introduction of appropriate therapy depends on rapid and accurate identification. The aims of this study were to compare the commercial identification systems namely API® ID 32C (bioMerieux, France) and Phoenix™ Yeast ID Panel (Becton Dickinson Diagnostics, USA) for the identification of Candida species and to evaluate the effect of morphological findings in the identification process. A total of 211 yeast strains isolated from different clinical samples (111 urine, 34 blood/vascular catheter, 27 upper/lower respiratory tract, 16 abscess/pus, 13 throat/vagina swabs and 10 sterile body fluids) of 137 patients hospitalized in Uludag University Health and Research Center between October 2013 to January 2014, were included in the study. Samples were cultured on blood agar, chromogenic agar (CHROMagar Candida, BD, USA) and Saboraud's dextrose agar (SDA), and isolated yeast colonies were evaluated with germ tube test and morphological examination by microscopy on cornmeal/Tween-80 agar. The isolates were identified as well by two commercial systems according to the manufacturers' recommendations. Discrepant results between the systems were tried to be resolved by using morphological characteristics of the yeasts. Of the isolates 159 were identified identical by both of the systems, and the concordance between those systems were estimated as 75.4%. According to the concordant identification, the most frequently isolated species was C.albicans (44.1%) followed by C.tropicalis (9.9%), C.glabrata (9.5%), C.parapsilosis (8.5%) and C.kefyr (8.1%). The concordance rate was 81.7% in identification of frequently isolated species (C.albicans, C.tropicalis, C.parapsilosis, C.glabrata, C

  15. [Comparison of Phoenix™ Yeast ID Panel and API® ID 32C commercial systems for the identification of Candida species isolated from clinical samples].

    PubMed

    Gayibova, Ülkü; Dalyan Cılo, Burcu; Ağca, Harun; Ener, Beyza

    2014-07-01

    Opportunistic fungal pathogens are one of the important causes of nosocomial infections, and several different types of yeasts, especially Candida species are increasingly recovered from immunocompromised patients. Since many of the yeasts are resistant to the commonly used antifungal agents, the introduction of appropriate therapy depends on rapid and accurate identification. The aims of this study were to compare the commercial identification systems namely API® ID 32C (bioMerieux, France) and Phoenix™ Yeast ID Panel (Becton Dickinson Diagnostics, USA) for the identification of Candida species and to evaluate the effect of morphological findings in the identification process. A total of 211 yeast strains isolated from different clinical samples (111 urine, 34 blood/vascular catheter, 27 upper/lower respiratory tract, 16 abscess/pus, 13 throat/vagina swabs and 10 sterile body fluids) of 137 patients hospitalized in Uludag University Health and Research Center between October 2013 to January 2014, were included in the study. Samples were cultured on blood agar, chromogenic agar (CHROMagar Candida, BD, USA) and Saboraud's dextrose agar (SDA), and isolated yeast colonies were evaluated with germ tube test and morphological examination by microscopy on cornmeal/Tween-80 agar. The isolates were identified as well by two commercial systems according to the manufacturers' recommendations. Discrepant results between the systems were tried to be resolved by using morphological characteristics of the yeasts. Of the isolates 159 were identified identical by both of the systems, and the concordance between those systems were estimated as 75.4%. According to the concordant identification, the most frequently isolated species was C.albicans (44.1%) followed by C.tropicalis (9.9%), C.glabrata (9.5%), C.parapsilosis (8.5%) and C.kefyr (8.1%). The concordance rate was 81.7% in identification of frequently isolated species (C.albicans, C.tropicalis, C.parapsilosis, C.glabrata, C

  16. Fresno County Mock Trial Competition.

    ERIC Educational Resources Information Center

    Fresno City Unified School District, CA.

    THE FOLLOWING IS THE FULL TEXT OF THIS DOCUMENT: The Fresno County Office of Education and the Fresno Unified School District hosted the Mock Trial Competition. The state competition is sponsored by the Constitutional Rights Foundation, with cosponsorship from the California State Bar Association and the California Young Lawyer's Association. This…

  17. Composting in Prince William County

    SciTech Connect

    Lyons, K.

    1995-10-01

    Hidden in a small industrial corner of Prince Williams County, in Northern Virginia, a composting facility, after its first flourishing year in business, has found itself part of a symbiotic triangle. Along with a landfill and a waste-to-energy (WTE) plant, the composting facility is one of three programs that make up a joint public-private venture and form a interjurisdictional solid waste/refuse exchange agreement. Faced with the prospects of having to close a landfill at the end of the year, a mandate on yard waste collection that was increasing collection tonnage, and no room for further landfill development, Fairfax County, with approximately 900,000 residents, needed help. In a turn-around situation, Prince William County--with approximately 240,000 residents, a low budget, and much space available for development--responded. Together the counties created a solid waste exchange. The basis for the program is a unity between the local governments on some solid waste issues and a composting facility. Composting, specifically yard waste composting, has been among the fastest-growing aspects of waste management. In 1990, the nation was composting 2% of its solid waste. By the end of 1995, according to the US EPA, between 4% and 7% of solid waste will be recovered through composting. The number of yard waste composting facilities operating has increased from 651 in 1988 to more than 3,000 in 1994.

  18. Somerset County Employer Needs Assessment.

    ERIC Educational Resources Information Center

    Rephann, Terance J.

    Allegany Community College in Cumberland, Maryland, conducted an employer assessment survey of Somerset County businesses during the winter of 1995 in order to provide evaluation data for planning and curriculum development for the secondary and postsecondary educational institutions. The survey was mailed to 760 establishments, with a 29 percent…

  19. Kinship Care in Walton County.

    ERIC Educational Resources Information Center

    Charon, Sara L.; Nackerud, Larry

    1996-01-01

    The quality of life of maltreated children placed with relatives was examined through interviews with nine families in Walton County (Georgia) who had taken in related children. Over half of the children had experienced some improvement in home life, school performance, and their physical and mental status. Kinship care families indicated needs…

  20. Southern Nevada Library Services; Serving Lincoln County, Nye County, Esmeralda County through the Clark County Library District: An Evaluation.

    ERIC Educational Resources Information Center

    Dalton, Phyllis I.

    An anecdotal review covers the first year of increased library service in Nye, Lincoln, and Esmeralda Counties, Nevada, under the Southern Nevada Library Services project funded by the Library Services and Construction Act. Using information from questionnaires and site visits, the extent of library services in each community in the area is…

  1. Truancy in Yolo County, California.

    ERIC Educational Resources Information Center

    Sommer, Barbara; Tyburczy, Jason

    The problem of truancy in the elementary and junior high schools of California's Yolo County was investigated during the 1980-81 academic year by means of questionnaires completed by 16 school principals and interviews with 30 people representing schools, school district offices, law enforcement agencies, the Department of Social Services, and the…

  2. Geothermal development plan: Pima County

    NASA Astrophysics Data System (ADS)

    White, D. H.; Goldstone, L. A.

    1982-08-01

    The Pima County Area Development evaluated the county-wide market potential for utilizing geothermal energy. Four potential geothermal resource areas with temperatures less than 1000 C (2120 F) were identified. In addition, one area is identified as having a temperature of 1470 F (2970 F). Geothermal resources are found to occur in Tecson where average population growth rates of two to three percent per year are expected over the next 40 years. Rapid growth in the manufacturing sector and the existence of major copper mines provide opportunities for the direct utilization of geothermal energy. However, available water supplies are identified as a major constraing to projected growth. A regional energy analysis, future predictions for energy consumption, and energy prices are given. Potential geothermal users in Pima County are identified and projections of maximum economic geothermal utilization are given. One hundred fifteen firms in 32 industrial classes have some potential for geothermal use are identified. In addition, 26 agribusiness firms were found in the county.

  3. Hydrogeology of Wood County, Wisconsin

    USGS Publications Warehouse

    Batten, W.G.

    1989-01-01

    The average rate of ground·water pumpage in Wood County in 1985 was 9.7 million gallons per day. Of this rate, about 6 million gallons per day is pumped from municipal-supply wells in seven communities.An additional 1.08 million gallons per day is pumped for agricultural irrigation.

  4. Measles in rural Ohio county.

    PubMed

    Orenstein, W A; Irvin, J; Jennings, M R; Giandelia, J; Halpin, T J; Marks, J S; Conrad, J L

    1980-06-01

    Between December 23, 1975, and March 31, 1976, 169 cases of measles were reported from Defiance County, Ohio, a small rural county in the northwest corner of the State. The outbreak spread from a single junior high school basketball player to eventually involve 19 of the 28 county schools. Among the affected schools, measles attack rates varied from 0.3-7.2% with a mean of 2.0%. A likely source of illness was determined for 160 of the 169 cases (95%). Intraschool transmission was most common, accounting for 97 of the 169 cases (57%) followed by sibling contact for 23 cases (14%). The pattern of measles spread was complex and would have been difficult to predict in advance even if surveillance systems reported each case the day it occurred. A control program held between February 2 and February 20, 1976, vaccinated 5145 of the 11,114 (46.3%) county schoolchildren. Forty-four cases of measles occurred 4 or more days following school clinics, 22 (50%) in children who requested measles vaccine at school clinics, 17 of whom were actually vaccinated. Most of the other cases occurred in students whose parents thought their children to be protected. Measles is a disease which spreads rapidly in a complex pattern over wide geographic areas. A control program vaccinating a large proportion of the children without definitive history of adequate vaccination or disease was apprently effective in curtailing the outbreak.

  5. Hydrogeology of Webb County, Texas

    USGS Publications Warehouse

    Lambert, Rebecca B.

    2004-01-01

    Introduction: Webb County, in semiarid South Texas on the U.S.-Mexico border, is a region confronted by increasing stresses on natural resources. Laredo (fig. 1), the largest city in Webb County (population 193,000 in 2000), was one of the 10 fastest-growing metropolitan areas in the country during 1990-2000 (Perry and Mackun, 2001). Commercial and industrial activities have expanded throughout the region to support the maquiladora industry (manufacturing plants in Mexico) along the border and other growth as a result of the passage of the North American Free Trade Agreement. The Rio Grande currently (2002) is the primary source of public water supply for Laredo and other cities along the border in Webb County (fig. 1). Other cities, such as Bruni and Mirando City in the southeastern part of the county, rely on ground-water supplies to meet municipal demands. Increased water demand associated with development and population growth in the region has increased the need for the City of Laredo and Webb County to evaluate alternative water sources to meet future demand. Possible options include (1) supplementing the surface-water supply with ground water, and (2) applying artificial storage and recovery (ASR) technology to recharge local aquifers. These options raise issues regarding the hydraulic capability of the aquifers to store economically substantial quantities of water, current or potential uses of the resource, and possible effects on the quality of water resulting from mixing ground water with alternative source waters. To address some of these issues, the U.S. Geological Survey (USGS), in cooperation with the City of Laredo, began a study in 1996 to assess the ground-water resources of Webb County. A hydrogeologic study was conducted to review and analyze available information on the hydrogeologic units (aquifers and confining units) in Webb County, to locate available wells in the region with water-level and water-quality information from the aquifers, and

  6. 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IDS) is encoded by multicopy genes in gymnosperms Ginkgo biloba and Pinus taeda.

    PubMed

    Kim, Sang-Min; Kuzuyama, Tomohisa; Kobayashi, Akio; Sando, Tomoki; Chang, Yung-Jin; Kim, Soo-Un

    2008-01-01

    Isoprenoids are synthesized through the condensation of five-carbon intermediates, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), derived from two distinct biosynthetic routes: cytosolic mevalonate (MVA) and plastidial 2-C-methyl-D: -erythritol 4-phosphate (MEP) pathways. 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IDS; EC 1.17.1.2), which catalyzes the last step of MEP pathway, was cloned as a multicopy gene from gymnosperms Ginkgo biloba (GbIDS1, GbIDS2, and GbIDS2-1) and Pinus taeda (PtIDS1 and PtIDS2), and characterized. Phylogenetic tree constructed with other plant IDSs demonstrated gymnosperm IDSs were distinctively different from angiosperm IDSs. The gymnosperm IDS clade contained two subclades, one composed of GbIDS1 and PtIDS1, and the other composed of GbIDS2, GbIDS2-1, and PtIDS2. G. biloba IDSs, except GbIDS2-1, successfully complemented Escherichia coli DLYT1, a lytB disruptant, confirming the in vivo competency of isozymes. During the 4 weeks study period, although transcript levels of GbIDS1s were similar both in roots and leaves of cultured G. biloba embryo, the transcripts of GbIDS2 predominantly occurred in the embryo roots, where diterpene ginkgolides are biosynthesized. Levels of PtIDS2 transcripts in the diterpenoid resin-producing wood were 4-5 times higher than those in other tissues. Higher levels of GbIDS1 transcripts were induced by light, whereas those of GbIDS2 were increased by methyl jasmonate treatment. These results strongly imply GbIDS2 and PtIDS2 have high correlation with secondary metabolism. In Arabidopsis transient expression system, N-terminal 100 amino acid residues of GbIDS1 delivered fused GFP protein into chloroplast as well as cytosol and nucleus, whereas those of GbIDS2, GbIDS2-1, and two PtIDSs delivered GFP only into chloroplast.

  7. Advanced Q-switched DPSS lasers for ID-card marking

    NASA Astrophysics Data System (ADS)

    Hertwig, Michael; Paster, Martin; Terbrueggen, Ralf

    2008-02-01

    Increased homeland security concerns across the world have generated a strong demand for forgery-proof ID documents. Manufacturers currently employ a variety of high technology techniques to produce documents that are difficult to copy. However, production costs and lead times are still a concern when considering any possible manufacturing technology. Laser marking has already emerged as an important tool in the manufacturer's arsenal, and is currently being utilized to produce a variety of documents, such as plastic ID cards, drivers' licenses, health insurance cards and passports. The marks utilized can range from simple barcodes and text to high resolution, true grayscale images. The technical challenges posed by these marking tasks include delivering adequate mark legibility, minimizing substrate burning or charring, accurately reproducing grayscale data, and supporting the required process throughput. This article covers the advantages and basic requirements on laser marking of cards and reviews how laser output parameters affect marking quality, speed and overall process economics.

  8. 2. Freud's 'id' and Jung's 'self' as aids in self-analysis.

    PubMed

    Plaut, Alfred B J

    2005-02-01

    The id and the self are described as constructs of our unconscious which can be deployed in describing the analytic process. They can be used like the x in mathematics or the joker in a pack of cards as fitting in almost anywhere. But we can call on them as important aids to concentration, when additional room is made for meditating on the past which then comes to life again. They are useful for analysing ourselves and others. In this way the id as well as the self become aide-mémoires. The analysis of the repressed by means of the transference is not the only road to hitherto unconscious memories. This kind of meditation has helped me despite all reservations to undertake self-analysis and to write an autobiography. I maintain that one can have a transference to oneself. It is, of course, narcissistic. But then, no one can write an autobiography without a healthy dose of narcissism.

  9. FLOR-ID: an interactive database of flowering-time gene networks in Arabidopsis thaliana.

    PubMed

    Bouché, Frédéric; Lobet, Guillaume; Tocquin, Pierre; Périlleux, Claire

    2016-01-01

    Flowering is a hot topic in Plant Biology and important progress has been made in Arabidopsis thaliana toward unraveling the genetic networks involved. The increasing complexity and the explosion of literature however require development of new tools for information management and update. We therefore created an evolutive and interactive database of flowering time genes, named FLOR-ID (Flowering-Interactive Database), which is freely accessible at http://www.flor-id.org. The hand-curated database contains information on 306 genes and links to 1595 publications gathering the work of >4500 authors. Gene/protein functions and interactions within the flowering pathways were inferred from the analysis of related publications, included in the database and translated into interactive manually drawn snapshots. PMID:26476447

  10. ROLE OF LEUCONOSTOC MESENTEROIDES IN LEAVENING THE BATTER OF IDLI, A FERMENTED FOOD OF INDIA.

    PubMed

    MUKHERJEE, S K; ALBURY, M N; PEDERSON, C S; VANVEEN, A G; STEINKRAUS, K H

    1965-03-01

    The fermentation of the batter of idli, a fermented food of India, was studied. The microorganisms responsible for the characteristic changes in the batter were isolated and identified. Although there is a sequential change in the bacterial flora, the predominant microorganism responsible for souring, as well as for gas production, was found to be Leuconostoc mesenteroides. In the later stages of fermentation, growth of Streptococcus faecalis and, still later, of Pediococcus cerevisiae becomes significant. The fermentation of idli demonstrates a leavening action caused by the activity of the heterofermentative lactic acid bacterium, L. mesenteroides. As far as is known, this is the first record of a leavening action produced exclusively by the activity of a lactic acid bacterium.

  11. FLOR-ID: an interactive database of flowering-time gene networks in Arabidopsis thaliana

    PubMed Central

    Bouché, Frédéric; Lobet, Guillaume; Tocquin, Pierre; Périlleux, Claire

    2016-01-01

    Flowering is a hot topic in Plant Biology and important progress has been made in Arabidopsis thaliana toward unraveling the genetic networks involved. The increasing complexity and the explosion of literature however require development of new tools for information management and update. We therefore created an evolutive and interactive database of flowering time genes, named FLOR-ID (Flowering-Interactive Database), which is freely accessible at http://www.flor-id.org. The hand-curated database contains information on 306 genes and links to 1595 publications gathering the work of >4500 authors. Gene/protein functions and interactions within the flowering pathways were inferred from the analysis of related publications, included in the database and translated into interactive manually drawn snapshots. PMID:26476447

  12. ROLE OF LEUCONOSTOC MESENTEROIDES IN LEAVENING THE BATTER OF IDLI, A FERMENTED FOOD OF INDIA.

    PubMed

    MUKHERJEE, S K; ALBURY, M N; PEDERSON, C S; VANVEEN, A G; STEINKRAUS, K H

    1965-03-01

    The fermentation of the batter of idli, a fermented food of India, was studied. The microorganisms responsible for the characteristic changes in the batter were isolated and identified. Although there is a sequential change in the bacterial flora, the predominant microorganism responsible for souring, as well as for gas production, was found to be Leuconostoc mesenteroides. In the later stages of fermentation, growth of Streptococcus faecalis and, still later, of Pediococcus cerevisiae becomes significant. The fermentation of idli demonstrates a leavening action caused by the activity of the heterofermentative lactic acid bacterium, L. mesenteroides. As far as is known, this is the first record of a leavening action produced exclusively by the activity of a lactic acid bacterium. PMID:14325884

  13. Role of Leuconostoc mesenteroides in Leavening the Batter of Idli, a Fermented Food of India1

    PubMed Central

    Mukherjee, S. K.; Albury, M. N.; Pederson, C. S.; Van Veen, A. G.; Steinkraus, K. H.

    1965-01-01

    The fermentation of the batter of idli, a fermented food of India, was studied. The microorganisms responsible for the characteristic changes in the batter were isolated and identified. Although there is a sequential change in the bacterial flora, the predominant microorganism responsible for souring, as well as for gas production, was found to be Leuconostoc mesenteroides. In the later stages of fermentation, growth of Streptococcus faecalis and, still later, of Pediococcus cerevisiae becomes significant. The fermentation of idli demonstrates a leavening action caused by the activity of the heterofermentative lactic acid bacterium, L. mesenteroides. As far as is known, this is the first record of a leavening action produced exclusively by the activity of a lactic acid bacterium. Images Fig. 3 PMID:14325884

  14. Synaptic and extrasynaptic traces of long-term memory: the ID molecule theory.

    PubMed

    Legéndy, Charles R

    2016-08-01

    It is generally assumed at the time of this writing that memories are stored in the form of synaptic weights. However, it is now also clear that the synapses are not permanent; in fact, synaptic patterns undergo significant change in a matter of hours. This means that to implement the long survival of distant memories (for several decades in humans), the brain must possess a molecular backup mechanism in some form, complete with provisions for the storage and retrieval of information. It is found below that the memory-supporting molecules need not contain a detailed description of mental entities, as had been envisioned in the 'memory molecule papers' from 50 years ago, they only need to contain unique identifiers of various entities, and that this can be achieved using relatively small molecules, using a random code ('ID molecules'). In this paper, the logistics of information flow are followed through the steps of storage and retrieval, and the conclusion reached is that the ID molecules, by carrying a sufficient amount of information (entropy), can effectively control the recreation of complex multineuronal patterns. In illustrations, it is described how ID molecules can be made to revive a selected cell assembly by waking up its synapses and how they cause a selected cell assembly to ignite by sending slow inward currents into its cells. The arrangement involves producing multiple copies of the ID molecules and distributing them at strategic locations at selected sets of synapses, then reaching them through small noncoding RNA molecules. This requires the quick creation of entropy-rich messengers and matching receptors, and it suggests that these are created from each other by small-scale transcription and reverse transcription. PMID:27206318

  15. Fine wavelength id for tunable laser local oscillators. [sensing the absorption emission spectra of atmospheric gases

    NASA Technical Reports Server (NTRS)

    Savage, M. G.; Augeri, R. C.

    1980-01-01

    A wavelength ID device which consists of an electronic show that the etalon has a finesse F 30 which is maintainable for several days. These tests also demonstrate that the etalon system is capable of resonance frequency stability during similar time periods. With currently available coatings, this level of performance is achievable over an optical bandwidth delta lambda = 3 micrometers centered at lambda = 10 micrometers.

  16. Preparation of idli batter, its properties and nutritional improvement during fermentation.

    PubMed

    Ghosh, Debasree; Chattopadhyay, Parimal

    2011-10-01

    Idli is a traditional fermented rice and black gram based food. Idli batter is prepared by soaking polished parboiled rice and decorticated black gram for 4 h at 30 ± 1 °C in water. The soaked mass was ground using a grinder with adequate amount of water. The blend ratios of 2:1, 3:1 and 4:1 (w/w) batter were allowed for fermentation for different periods with the addition of 2% (w/w) of salt. The rheology of the product was assessed using a Brookfield Viscometer having disc spindles. Shear stress values were in the range of 0.22 and 4 Pa and reached a maximum value at 7 h of fermentation. The density, pH, and percentage total acidity of batter during fermentation for different blend ratios ranged between 0.93 and 0.59 gm cm(-3), 4.21 and 5.9 and 0.44 and 0.91% respectively. During fermentation, maximum production of riboflavin and thiamine were found to be 0.76 mg/100 gm and 0.73 mg/100 gm in 3:1 blend ratio of idli batter; and the folic acid content was found to be at a maximum of 0.75 mg/100 gm of idli batter after 10 h of fermentation. Digestibility in terms of amino N2 content was analysed by formol titration.

  17. Evaluation of the Organon-Teknika MICRO-ID LISTERIA system.

    PubMed

    Bannerman, E; Yersin, M N; Bille, J

    1992-06-01

    The MICRO-ID LISTERIA system, designed to identify Listeria isolates to species level within 24 h, was compared with conventional biochemical identification. MICRO-ID LISTERIA used in combination with the CAMP test correctly identified 409 (98.8%) of 414 strains isolated from human, animal, food, and environmental sources belonging to the seven species currently defined within the genus Listeria. The kit was easy to use and simple to interpret. However, 8 of the 15 tests (i.e., phenylalanine deaminase, hydrogen sulfide, indole, ornithine decarboxylase, lysine decarboxylase, malonate, urease, and o-nitrophenyl-beta-D-galactopyranoside) were considered superfluous for the differentiation of Listeria spp. The CAMP test was indispensable when using the MICRO-ID LISTERIA system, in particular to differentiate CAMP test-positive L. monocytogenes from the nonhemolytic, rhamnose-positive L. innocua. The hemolytic L. seeligeri and L. ivanovii strains and the nonhemolytic, non-rhamnose-acidifying L. welshimeri strains could also be differentiated from one another only on the basis of their CAMP test results. The very few strains of L. grayi and L. murrayi were easily differentiated from the other nonhemolytic species. Catalase-negative cocci should not be tested, because 12 out of 19 catalase-negative strains (all enterococci) in our test were misidentified as Listeria spp. The MICRO-ID LISTERIA system identified strains within 18 to 24 h and is thus less time-consuming than conventional tests. The system could, therefore, be used together with correctly done CAMP tests for the rapid identification of Listeria isolates, especially food and environmental isolates, for which rapid species differentiation is important.

  18. Evaluation of the Organon-Teknika MICRO-ID LISTERIA system.

    PubMed Central

    Bannerman, E; Yersin, M N; Bille, J

    1992-01-01

    The MICRO-ID LISTERIA system, designed to identify Listeria isolates to species level within 24 h, was compared with conventional biochemical identification. MICRO-ID LISTERIA used in combination with the CAMP test correctly identified 409 (98.8%) of 414 strains isolated from human, animal, food, and environmental sources belonging to the seven species currently defined within the genus Listeria. The kit was easy to use and simple to interpret. However, 8 of the 15 tests (i.e., phenylalanine deaminase, hydrogen sulfide, indole, ornithine decarboxylase, lysine decarboxylase, malonate, urease, and o-nitrophenyl-beta-D-galactopyranoside) were considered superfluous for the differentiation of Listeria spp. The CAMP test was indispensable when using the MICRO-ID LISTERIA system, in particular to differentiate CAMP test-positive L. monocytogenes from the nonhemolytic, rhamnose-positive L. innocua. The hemolytic L. seeligeri and L. ivanovii strains and the nonhemolytic, non-rhamnose-acidifying L. welshimeri strains could also be differentiated from one another only on the basis of their CAMP test results. The very few strains of L. grayi and L. murrayi were easily differentiated from the other nonhemolytic species. Catalase-negative cocci should not be tested, because 12 out of 19 catalase-negative strains (all enterococci) in our test were misidentified as Listeria spp. The MICRO-ID LISTERIA system identified strains within 18 to 24 h and is thus less time-consuming than conventional tests. The system could, therefore, be used together with correctly done CAMP tests for the rapid identification of Listeria isolates, especially food and environmental isolates, for which rapid species differentiation is important. PMID:1622280

  19. A non-parametric peak calling algorithm for DamID-Seq.

    PubMed

    Li, Renhua; Hempel, Leonie U; Jiang, Tingbo

    2015-01-01

    Protein-DNA interactions play a significant role in gene regulation and expression. In order to identify transcription factor binding sites (TFBS) of double sex (DSX)-an important transcription factor in sex determination, we applied the DNA adenine methylation identification (DamID) technology to the fat body tissue of Drosophila, followed by deep sequencing (DamID-Seq). One feature of DamID-Seq data is that induced adenine methylation signals are not assured to be symmetrically distributed at TFBS, which renders the existing peak calling algorithms for ChIP-Seq, including SPP and MACS, inappropriate for DamID-Seq data. This challenged us to develop a new algorithm for peak calling. A challenge in peaking calling based on sequence data is estimating the averaged behavior of background signals. We applied a bootstrap resampling method to short sequence reads in the control (Dam only). After data quality check and mapping reads to a reference genome, the peaking calling procedure compromises the following steps: 1) reads resampling; 2) reads scaling (normalization) and computing signal-to-noise fold changes; 3) filtering; 4) Calling peaks based on a statistically significant threshold. This is a non-parametric method for peak calling (NPPC). We also used irreproducible discovery rate (IDR) analysis, as well as ChIP-Seq data to compare the peaks called by the NPPC. We identified approximately 6,000 peaks for DSX, which point to 1,225 genes related to the fat body tissue difference between female and male Drosophila. Statistical evidence from IDR analysis indicated that these peaks are reproducible across biological replicates. In addition, these peaks are comparable to those identified by use of ChIP-Seq on S2 cells, in terms of peak number, location, and peaks width.

  20. A non-parametric peak calling algorithm for DamID-Seq.

    PubMed

    Li, Renhua; Hempel, Leonie U; Jiang, Tingbo

    2015-01-01

    Protein-DNA interactions play a significant role in gene regulation and expression. In order to identify transcription factor binding sites (TFBS) of double sex (DSX)-an important transcription factor in sex determination, we applied the DNA adenine methylation identification (DamID) technology to the fat body tissue of Drosophila, followed by deep sequencing (DamID-Seq). One feature of DamID-Seq data is that induced adenine methylation signals are not assured to be symmetrically distributed at TFBS, which renders the existing peak calling algorithms for ChIP-Seq, including SPP and MACS, inappropriate for DamID-Seq data. This challenged us to develop a new algorithm for peak calling. A challenge in peaking calling based on sequence data is estimating the averaged behavior of background signals. We applied a bootstrap resampling method to short sequence reads in the control (Dam only). After data quality check and mapping reads to a reference genome, the peaking calling procedure compromises the following steps: 1) reads resampling; 2) reads scaling (normalization) and computing signal-to-noise fold changes; 3) filtering; 4) Calling peaks based on a statistically significant threshold. This is a non-parametric method for peak calling (NPPC). We also used irreproducible discovery rate (IDR) analysis, as well as ChIP-Seq data to compare the peaks called by the NPPC. We identified approximately 6,000 peaks for DSX, which point to 1,225 genes related to the fat body tissue difference between female and male Drosophila. Statistical evidence from IDR analysis indicated that these peaks are reproducible across biological replicates. In addition, these peaks are comparable to those identified by use of ChIP-Seq on S2 cells, in terms of peak number, location, and peaks width. PMID:25785608

  1. Preparation of idli batter, its properties and nutritional improvement during fermentation.

    PubMed

    Ghosh, Debasree; Chattopadhyay, Parimal

    2011-10-01

    Idli is a traditional fermented rice and black gram based food. Idli batter is prepared by soaking polished parboiled rice and decorticated black gram for 4 h at 30 ± 1 °C in water. The soaked mass was ground using a grinder with adequate amount of water. The blend ratios of 2:1, 3:1 and 4:1 (w/w) batter were allowed for fermentation for different periods with the addition of 2% (w/w) of salt. The rheology of the product was assessed using a Brookfield Viscometer having disc spindles. Shear stress values were in the range of 0.22 and 4 Pa and reached a maximum value at 7 h of fermentation. The density, pH, and percentage total acidity of batter during fermentation for different blend ratios ranged between 0.93 and 0.59 gm cm(-3), 4.21 and 5.9 and 0.44 and 0.91% respectively. During fermentation, maximum production of riboflavin and thiamine were found to be 0.76 mg/100 gm and 0.73 mg/100 gm in 3:1 blend ratio of idli batter; and the folic acid content was found to be at a maximum of 0.75 mg/100 gm of idli batter after 10 h of fermentation. Digestibility in terms of amino N2 content was analysed by formol titration. PMID:23572795

  2. Expression of Id proteins is regulated by the Bcl-3 proto-oncogene in prostate cancer.

    PubMed

    Ahlqvist, K; Saamarthy, K; Syed Khaja, A S; Bjartell, A; Massoumi, R

    2013-03-21

    B-cell leukemia 3 (Bcl-3) is a member of the inhibitor of κB family, which regulates a wide range of biological processes by functioning as a transcriptional activator or as a repressor of target genes. As high levels of Bcl-3 expression and activation have been detected in different types of human cancer, Bcl-3 has been labeled a proto-oncogene. Our study uncovered a markedly upregulated Bcl-3 expression in human prostate cancer (PCa), where inflammatory cell infiltration was observed. Elevated Bcl-3 expression in PCa was dependent on the proinflammatory cytokine interleukin-6-mediated STAT3 activation. Microarray analyses, using Bcl-3 knockdown in PCa cells, identified the inhibitor of DNA-binding (Id) family of helix-loop-helix proteins as potential Bcl-3-regulated genes. Bcl-3 knockdown reduced the abundance of Id-1 and Id-2 proteins and boosted PCa cells to be more receptive to undergoing apoptosis following treatment with anticancer drug. Our data imply that inactivation of Bcl-3 may lead to sensitization of cancer cells to chemotherapeutic drug-induced apoptosis, thus suggesting a potential therapeutic strategy in PCa treatment.

  3. RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences.

    PubMed

    Brule, C E; Dean, K M; Grayhack, E J

    2016-01-01

    The identification and analysis of sequences that regulate gene expression is critical because regulated gene expression underlies biology. RNA-ID is an efficient and sensitive method to discover and investigate regulatory sequences in the yeast Saccharomyces cerevisiae, using fluorescence-based assays to detect green fluorescent protein (GFP) relative to a red fluorescent protein (RFP) control in individual cells. Putative regulatory sequences can be inserted either in-frame or upstream of a superfolder GFP fusion protein whose expression, like that of RFP, is driven by the bidirectional GAL1,10 promoter. In this chapter, we describe the methodology to identify and study cis-regulatory sequences in the RNA-ID system, explaining features and variations of the RNA-ID reporter, as well as some applications of this system. We describe in detail the methods to analyze a single regulatory sequence, from construction of a single GFP variant to assay of variants by flow cytometry, as well as modifications required to screen libraries of different strains simultaneously. We also describe subsequent analyses of regulatory sequences.

  4. Purification, crystallization and preliminary crystallographic study of an IDS-epimerase from Agrobacterium tumefaciens BY6

    SciTech Connect

    Bäuerle, Bettina; Sandalova, Tatyana; Schneider, Gunter; Rieger, Paul-Gerhard

    2006-08-01

    This is the first report of the crystallization of an IDS-epimerase from A. tumefaciens BY6 and its l-selenomethionine derivative. The initial degradation of all stereoisomers of the complexing agent iminodisuccinate (IDS) is enabled by an epimerase in the bacterial strain Agrobacterium tumefaciens BY6. This protein was produced in Escherichia coli, purified and crystallized by the hanging-drop vapour-diffusion method. Crystals of IDS-epimerase were obtained under several conditions. The best diffracting crystals were grown in 22% PEG 3350, 0.2 M (NH{sub 4}){sub 2}SO{sub 4} and 0.1 M bis-Tris propane pH 7.2 at 293 K. These crystals belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 55.4, b = 104.2, c = 78.6 Å, β = 103.3°, and diffracted to 1.7 Å resolution. They contain two protein molecules per asymmetric unit. In order to solve the structure using the MAD phasing method, crystals of the l-selenomethionine-substituted epimerase were grown in the presence of 20% PEG 3350, 0.2 M Na{sub 2}SO{sub 4} and 0.1 M bis-Tris propane pH 8.5.

  5. Towards fraud-proof ID documents using multiple data hiding technologies and biometrics

    NASA Astrophysics Data System (ADS)

    Picard, Justin; Vielhauer, Claus; Thorwirth, Niels

    2004-06-01

    Identity documents, such as ID cards, passports, and driver's licenses, contain textual information, a portrait of the legitimate holder, and eventually some other biometric characteristics such as a fingerprint or handwritten signature. As prices for digital imaging technologies fall, making them more widely available, we have seen an exponential increase in the ease and the number of counterfeiters that can effectively forge documents. Today, with only limited knowledge of technology and a small amount of money, a counterfeiter can effortlessly replace a photo or modify identity information on a legitimate document to the extent that it is very diffcult to differentiate from the original. This paper proposes a virtually fraud-proof ID document based on a combination of three different data hiding technologies: digital watermarking, 2-D bar codes, and Copy Detection Pattern, plus additional biometric protection. As will be shown, that combination of data hiding technologies protects the document against any forgery, in principle without any requirement for other security features. To prevent a genuine document to be used by an illegitimate user,biometric information is also covertly stored in the ID document, to be used for identification at the detector.

  6. Validating crustal velocities derived from the IDS contribution to ITRF201

    NASA Astrophysics Data System (ADS)

    Moreaux, G.; Soudarin, L.; Gravelle, M.; Santamaria Gomez, A.; Lemoine, F. G.; Willis, P.; Ferrage, P.; Argus, D. F.

    2015-12-01

    In the context of the ITRF2014, the IDS Combination Center delivered 1140 weekly SINEX files to the IERS containing DORIS station positions and Earth orientation parameters from January 1993 to December 2014 (series IDS 09). For each DORIS station, we computed the mean position and velocity by stacking the IDS 09 weekly solutions and combining them with both DORIS internal local ties (3-D vector between two successive beacon installations at the same DORIS site) from IGN and a discontinuity file. The first objective of this study is to compare DORIS horizontal velocities to estimations from tectonic models such as GEODVEL and NNR-MORVEL56. The second purpose is to evaluate DORIS vertical velocities with respect to Global Isostatic Adjustment models (for Greenland, Fennoscandia, Iceland and Antarctica sites) such as ICE-6G_C, and with respect to the latests GPS solution at the University of La Rochelle. Sites showing large differences in either horizontal (ex: Arequipa, Dionysos/Gavdos, Goldstone, Manila and Santiago) or vertical velocities (ex: Rothera, Thule and Yellowknife) will be of special concern.

  7. The International DORIS Service (IDS) - Recent Developments in Preparation for ITRF2013

    NASA Technical Reports Server (NTRS)

    Willis, Pascal; Lemoine, Frank G.; Moreaux, Guilhem; Soudarin, Laurent; Ferrage, Pascale; Ries, John; Otten, Michiel; Saunier, Jerome; Noll, Carey E.; Biancale, Richard; Luzum, Brian

    2014-01-01

    The International DORIS Service (IDS) was created in 2003 under the umbrella of the International Association of Geodesy (IAG) to foster scientific research related to the French DORIS tracking system and to deliver scientific products, mostly related to the International Earth rotation and Reference systems Service (IERS). We first present some general background related to the DORIS system (current and planned satellites, current tracking network and expected evolution) and to the general IDS organization (from Data Centers, Analysis Centers and Combination Center). Then, we discuss some of the steps recently taken to prepare the IDS submission to ITRF2013 (combined weekly time series based on individual solutions from several Analysis Centers). In particular, recent results obtained from the Analysis Centers and the Combination Center show that improvements can still be made when updating physical models of some DORIS satellites, such as Envisat, Cryosat-2 or Jason-2. The DORIS contribution to ITRF2013 should also benefit from the larger number of ground observations collected by the last generation of DGXX receivers (first instrument being onboard Jason-2 satellite). In particular for polar motion, sub-millarcsecond accuracy seems now to be achievable. Weekly station positioning internal consistency also seems to be improved with a larger DORIS constellation.

  8. Association of ACE Gene I/D polymorphism with migraine in Kashmiri population

    PubMed Central

    Wani, Irfan Yousuf; Sheikh, Saleem; Shah, Zafar Amin; Pandith, Arshid A.; Wani, Mushtaq; Asimi, Ravouf; Wani, Maqbool; Sheikh, Shahnawaz; Mehraj, Iqra

    2016-01-01

    Introduction: Migraine is a complex, recurrent headache disorder that is one of the most common complaints in neurology practice. The role of various genes in its pathogenesis is being studied. We did this study to see whether an association exists between ACE gene I/D polymorphism and migraine in our region. Materials and Methods: The study included 100 patients diagnosed with migraine and 121 healthy controls. The study subject were age and gender matched. The analysis was based on Polymerase Chain Reaction (PCR) and included following steps: DNA extraction from blood, PCR and Restriction Fragment Length Polymorphism (RFLP). Results: Out of 100 cases, 69 were females and 31 were males. Fifty-seven were having migraine without aura and 43 had migraine with aura. 45 of the cases had II polymorphism, 40 had ID polymorphism and 15 had DD polymorphism in ACE gene. Conclusion: We were not able to find a statistically significant association between ACE gene I/D polymorphism with migraine. The reason for difference in results between our study and other studies could be because of different ethnicity in study populations. So a continuous research is needed in this regard in order to find the genes and different polymorphism that increase the susceptibility of Kashmiri population to migraine. PMID:27011636

  9. Design and Technical Aspects of a New in Vacuum Transfocator at ESRF Beamline ID11

    SciTech Connect

    Rossat, M.; Vaughan, G. B. M.; Snigirev, A.; Wright, J. P; Bytchkov, A.; Dabin, Y.; Zhang, L.

    2010-06-23

    A tunable X-Ray focusing apparatus based on compound refractive lenses (CRL), referred to as a transfocator, has been installed for monochromatic beam (IAT in air transfocator) two years ago in the second optics hutch at ID11 at the ESRF. By varying the number of lenses in the beam, the energy focused and the focal length can be varied continuously throughout a large range of energies and distances.Based the success of the IAT, an in-vacuum transfocator (IVT) has now been built and installed at ID11.The aim of this poster is to describe and show the mechanical and technical solutions used to design and manufacture this new IVT.The IVT was installed last January in the first optics hutch at ID11. The IVT consists of nine water cooled cartridges containing 1, 2, 4, 8, 16, 32 Be lenses, and 32 and 64 Al lenses, and a protective aperture of Oe 900 {mu}m. The mechanical design is based on a system of pneumatically actuated cartridges with an auto positioning on two stainless steel rods. The vacuum vessel has translations and rotations perpendicular to the beam direction in order to align the axis of the cartridges with the x-ray beam.

  10. Node ID based detection of Sybil attack in mobile wireless sensor network

    NASA Astrophysics Data System (ADS)

    Sharmila, S.; Umamaheswari, G.

    2013-10-01

    Security is the major issue in wireless sensor networks and many defence mechanisms have been developed to secure the network from these alarming attacks by detecting the malicious nodes which hinder the performance of the network. Sybil attack can make the network vulnerable. Sybil attack means a node which illegitimately claims multiple identities. This attack threatens wireless sensor network in routing, voting system, fair resource allocation, data aggregation and misbehaviour detection. Hence, the research is carried out to prevent the Sybil attack and improve the network performance. The node ID-based scheme is proposed, where the detection is based on node registration, consisting of two phases and the assignment of ID to the node is done dynamically. The ID's corresponding to the nodes registered is at the base station and the node active time is monitored, any abnormalities in the above phases confirm the presence of Sybil nodes in the network. The scheme is simulated using NS2. The energy consumed for this algorithm is 2.3 J. The proposed detection scheme is analysed based on the network's PDR and found that the throughput has improved, which prove that this scheme may be used in the environment where security is needed.

  11. Enigmatic in vivo iduronate-2-sulfatase (IDS) mutant transcript correction to wild-type in Hunter syndrome.

    PubMed

    Lualdi, Susanna; Tappino, Barbara; Di Duca, Marco; Dardis, Andrea; Anderson, Christopher J; Biassoni, Roberto; Thompson, Peter W; Corsolini, Fabio; Di Rocco, Maja; Bembi, Bruno; Regis, Stefano; Cooper, David N; Filocamo, Mirella

    2010-04-01

    Sequence analysis of the X-linked iduronate-2-sulfatase (IDS) gene in two Hunter syndrome patients revealed a lack of concordance between IDS genomic DNA and cDNA. These individuals were found to be hemizygous respectively for a nonsense mutation [c.22C>T;p.R8X] and a frameshift micro-insertion [c.10insT;p.P4Sfs] in their genomic DNA. However, both wild-type and mutant IDS sequences were evident upon cDNA analysis. Similar discrepant results were also obtained in a third unrelated patient carrying the same p.R8X mutation. Since both p.R8X mutations were inherited from carrier mothers, somatic mosaicism could be excluded. Although the presence of wild-type IDSmRNA-transcripts was confirmed in all three patients by restriction enzyme digestion, clone sequencing, pyrosequencing and single nucleotide primer extension (SNuPE), no wild-type IDS genomic sequence was detectable. The relative abundance of wild-type and mutation-bearing IDS-transcripts in different tissues was quantified by SNuPE. Although IDS transcript levels, as measured by real-time PCR, were reduced (51-71% normal) in these patients, some wild-type IDS protein was detectable by western blotting. Various possible explanations for these unprecedented findings (e.g. accidental contamination, artefactual in vitro nucleotide misincorporation, malsegregation of an extra maternal X-chromosome) were explored and experimentally excluded. PCR-based discriminant assay and segregation analysis of a linked IDS polymorphism (rs1141608) also served to exclude the presence of IDS cDNA derived from the maternal wild-type chromosome. Although it remains to be formally demonstrated by direct experimentation, the intriguing possibility arises that we have observed the in vivo correction of heritable gene lesions at the RNA level operating via a correction mechanism akin to RNA-editing. (c) 2010 Wiley-Liss, Inc.

  12. Id4, a New Candidate Gene for Senile Osteoporosis, Acts as a Molecular Switch Promoting Osteoblast Differentiation

    PubMed Central

    Yamashita, Yzumi; Nakachi, Yutaka; Nikaido, Itoshi; Bono, Hidemasa; Ninomiya, Yuichi; Kanesaki-Yatsuka, Yukiko; Akita, Masumi; Motegi, Hiromi; Wakana, Shigeharu; Noda, Tetsuo; Sablitzky, Fred; Arai, Shigeki; Kurokawa, Riki; Fukuda, Toru; Katagiri, Takenobu; Schönbach, Christian; Suda, Tatsuo; Mizuno, Yosuke; Okazaki, Yasushi

    2010-01-01

    Excessive accumulation of bone marrow adipocytes observed in senile osteoporosis or age-related osteopenia is caused by the unbalanced differentiation of MSCs into bone marrow adipocytes or osteoblasts. Several transcription factors are known to regulate the balance between adipocyte and osteoblast differentiation. However, the molecular mechanisms that regulate the balance between adipocyte and osteoblast differentiation in the bone marrow have yet to be elucidated. To identify candidate genes associated with senile osteoporosis, we performed genome-wide expression analyses of differentiating osteoblasts and adipocytes. Among transcription factors that were enriched in the early phase of differentiation, Id4 was identified as a key molecule affecting the differentiation of both cell types. Experiments using bone marrow-derived stromal cell line ST2 and Id4-deficient mice showed that lack of Id4 drastically reduces osteoblast differentiation and drives differentiation toward adipocytes. On the other hand knockdown of Id4 in adipogenic-induced ST2 cells increased the expression of Pparγ2, a master regulator of adipocyte differentiation. Similar results were observed in bone marrow cells of femur and tibia of Id4-deficient mice. However the effect of Id4 on Pparγ2 and adipocyte differentiation is unlikely to be of direct nature. The mechanism of Id4 promoting osteoblast differentiation is associated with the Id4-mediated release of Hes1 from Hes1-Hey2 complexes. Hes1 increases the stability and transcriptional activity of Runx2, a key molecule of osteoblast differentiation, which results in an enhanced osteoblast-specific gene expression. The new role of Id4 in promoting osteoblast differentiation renders it a target for preventing the onset of senile osteoporosis. PMID:20628571

  13. Chester County ground-water atlas, Chester County, Pennsylvania

    USGS Publications Warehouse

    Ludlow, Russell A.; Loper, Connie A.

    2004-01-01

    Chester County encompasses 760 square miles in southeastern Pennsylvania. Groundwater- quality studies have been conducted in the county over several decades to address specific hydrologic issues. This report compiles and describes water-quality data collected during studies conducted mostly after 1990 and summarizes the data in a county-wide perspective. In this report, water-quality constituents are described in regard to what they are, why the constituents are important, and where constituent concentrations vary relative to geology or land use. Water-quality constituents are grouped into logical units to aid presentation: water-quality constituents measured in the field (pH, alkalinity, specific conductance, and dissolved oxygen), common ions, metals, radionuclides, bacteria, nutrients, pesticides, and volatile organic compounds.Waterquality constituents measured in the field, common ions (except chloride), metals, and radionuclides are discussed relative to geology. Bacteria, nutrients, pesticides, and volatile organic compounds are discussed relative to land use. If the U.S. Environmental Protection Agency (USEPA) or Chester County Health Department has drinkingwater standards for a constituent, the standards are included. Tables and maps are included to assist Chester County residents in understanding the water-quality constituents and their distribution in the county. Ground water in Chester County generally is of good quality and is mostly acidic except in the carbonate rocks and serpentinite, where it is neutral to strongly basic. Calcium carbonate and magnesium carbonate are major constituents of these rocks. Both compounds have high solubility, and, as such, both are major contributors to elevated pH, alkalinity, specific conductance, and the common ions. Elevated pH and alkalinity in carbonate rocks and serpentinite can indicate a potential for scaling in water heaters and household plumbing. Low pH and low alkalinity in the schist, quartzite, and

  14. The Transcriptional Repressor ID2 Can Interact with the Canonical Clock Components CLOCK and BMAL1 and Mediate Inhibitory Effects on mPer1 Expression*

    PubMed Central

    Ward, Sarah M.; Fernando, Shanik J.; Hou, Tim Y.; Duffield, Giles E.

    2010-01-01

    ID2 is a rhythmically expressed HLH transcriptional repressor. Deletion of Id2 in mice results in circadian phenotypes, highlighted by disrupted locomotor activity rhythms and an enhanced photoentrainment response. ID2 can suppress the transactivation potential of the positive elements of the clock, CLOCK-BMAL1, on mPer1 and clock-controlled gene (CCG) activity. Misregulation of CCGs is observed in Id2−/− liver, and mutant mice exhibit associated alterations in lipid homeostasis. These data suggest that ID2 contributes to both input and output components of the clock and that this may be via interaction with the bHLH clock proteins CLOCK and BMAL1. The aim of the present study was to explore this potential interaction. Coimmunoprecipitation analysis revealed the capability of ID2 to complex with both CLOCK and BMAL1, and mammalian two-hybrid analysis revealed direct interactions of ID2, ID1 and ID3 with CLOCK and BMAL1. Deletion of the ID2 HLH domain rendered ID2 ineffective at inhibiting CLOCK-BMAL1 transactivation, suggesting that interaction between the proteins is via the HLH region. Immunofluorescence analysis revealed overlapping localization of ID2 with CLOCK and BMAL1 in the cytoplasm. Overexpression of CLOCK and BMAL1 in the presence of ID2 resulted in a significant reduction in their nuclear localization, revealing that ID2 can sequester CLOCK and BMAL1 to the cytoplasm. Serum stimulation of Id2−/− mouse embryonic fibroblasts resulted in an enhanced induction of mPer1 expression. These data provide the basis for a molecular mechanism through which ID2 could regulate aspects of both clock input and output through a time-of-day specific interaction with CLOCK and BMAL1. PMID:20861012

  15. You can be a wizard of IDS. Is your practice ready to form an integrated delivery system?

    PubMed

    Fabrizio, Nick A

    2007-09-01

    To combat declining reimbursement and strengthen their positions in a highly competitive provider environment, leaders of large medical groups may consider forming an integrate delivery system (IDS) to gain a business advantage. Managing the array of organizational, operational, financial and human relationships affected by an IDS requires careful planning, good timing and a bit of luck. This article, the first of a two-part series, examines what questions physician practice leaders should ask--and answer--before formiing an IDS. PMID:17910216

  16. 6 CFR 37.71 - Driver's licenses and identification cards issued under section 202(d)(11) of the REAL ID Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Driver's Licenses and Identification Cards Issued Under Section 202(d)(11) of the REAL ID Act § 37.71 Driver's licenses and identification cards issued under section 202(d)(11) of the REAL ID Act. (a) Except...

  17. 6 CFR 37.71 - Driver's licenses and identification cards issued under section 202(d)(11) of the REAL ID Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... issued under section 202(d)(11) of the REAL ID Act. 37.71 Section 37.71 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REAL ID DRIVER'S LICENSES AND IDENTIFICATION CARDS Driver's Licenses and Identification Cards Issued Under section 202(d)(11) of the REAL ID Act § 37.71...

  18. Frontal Brain Electrical Activity (EEG) and Heart Rate in Response to Affective Infant-Directed (ID) Speech in 9-Month-Old Infants

    ERIC Educational Resources Information Center

    Santesso, Diane L.; Schmidt, Louis A.; Trainor, Laurel J.

    2007-01-01

    Many studies have shown that infants prefer infant-directed (ID) speech to adult-directed (AD) speech. ID speech functions to aid language learning, obtain and/or maintain an infant's attention, and create emotional communication between the infant and caregiver. We examined psychophysiological responses to ID speech that varied in affective…

  19. DnsID in MyCompoundID for rapid identification of dansylated amine- and phenol-containing metabolites in LC-MS-based metabolomics.

    PubMed

    Huan, Tao; Wu, Yiman; Tang, Chenqu; Lin, Guohui; Li, Liang

    2015-10-01

    High-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) is an enabling technology based on rational design of labeling reagents to target a class of metabolites sharing the same functional group (e.g., all the amine-containing metabolites or the amine submetabolome) to provide concomitant improvements in metabolite separation, detection, and quantification. However, identification of labeled metabolites remains to be an analytical challenge. In this work, we describe a library of labeled standards and a search method for metabolite identification in CIL LC-MS. The current library consists of 273 unique metabolites, mainly amines and phenols that are individually labeled by dansylation (Dns). Some of them produced more than one Dns-derivative (isomers or multiple labeled products), resulting in a total of 315 dansyl compounds in the library. These metabolites cover 42 metabolic pathways, allowing the possibility of probing their changes in metabolomics studies. Each labeled metabolite contains three searchable parameters: molecular ion mass, MS/MS spectrum, and retention time (RT). To overcome RT variations caused by experimental conditions used, we have developed a calibration method to normalize RTs of labeled metabolites using a mixture of RT calibrants. A search program, DnsID, has been developed in www.MyCompoundID.org for automated identification of dansyl labeled metabolites in a sample based on matching one or more of the three parameters with those of the library standards. Using human urine as an example, we illustrate the workflow and analytical performance of this method for metabolite identification. This freely accessible resource is expandable by adding more amine and phenol standards in the future. In addition, the same strategy should be applicable for developing other labeled standards libraries to cover different classes of metabolites for comprehensive metabolomics using CIL LC-MS.

  20. Ground-water potentialities in the Crescent Valley, Eureka and Lander Counties, Nevada

    USGS Publications Warehouse

    Zones, Christie Paul

    1961-01-01

    The Crescent Valley is an intermontane basin in Eureka and Lander Counties, just south of the Humboldt River in north-central Nevada. The valley floor, with an area of about 150 square miles, has a shape that more nearly resembles a Y than a crescent, although the valley apparently was named after the arc described by its southern part and northeastern arm. The northwestern arm of the Y extends northward to the small railroad town of Beowawe on the Humboldt River; the northeastern arm lies east of the low Dry Hills. The leg of the Y extends southwestward toward a narrow gap which separates the Crescent Valley from the Carico Lake Valley. The total drainage area of the Crescent Valley-about 700 square miles--includes also the slopes of the bordering mountain ranges: the Shoshone Range to the west, the Cortez Mountains to the east, and the Toiyabe Range to the south. The early history of the Crescent Valley was dominated by mining of silver and gold, centered at Lander in the Shoshone Range and at Cortez and Mill Canyon in the Cortez Mountains, but in recent years the only major mining activity has been at Gold Acres; there open-pit mining of low-grade gold ore has supported a community of about 200. For many years the only agricultural enterprises in the valley were two cattle ranches, but recently addition lands have been developed for the raising of crops in the west-central part of the valley. The average annual precipitation upon the floor of the Crescent Valley is probably less than 7 inches, of which only a little more than 1 inch formally falls during the growing season (from June through September). This is far less than the requirement of any plants of economic value, and irrigation is essential to agricultural development. Small perennial streams rising in the mountains have long been utilized for domestic supply, mining and milling activities of the past, and irrigation, and recently some large wells have been developed for irrigation. In 1956 the total

  1. Determination of trace elements on polysilicates by ID-ICP-MS with ultrasonic nebulization/membrane desolvation

    SciTech Connect

    Bonchin-Cleland, S.; Olivares, J.A.; Miller, G.G.; Gallegos, L.; Dawson, H.J.

    1996-06-01

    This work investigates the performance of an ID-ICP-MS (isotope dilution-inductively coupled plasma mass spectrometry) system with USN/MD (ultrasonic nebulization/membrane desolvation) sample introduction for the determination of trace metal impurities in polysilicon.

  2. Earth observing system. Output data products and input requirements, version 2.0. Volume 2: Analysis of IDS input requirements

    NASA Technical Reports Server (NTRS)

    Lu, Yun-Chi; Chang, Hyo Duck; Krupp, Brian; Kumar, Ravindra; Swaroop, Anand

    1992-01-01

    On 18 Jan. 1991, NASA confirmed 29 Inter-Disciplinary Science (IDS) teams, each involving a group of investigators, to conduct interdisciplinary research using data products from Earth Observing System (EOS) instruments. These studies are multi-disciplinary and require output data products from multiple EOS instruments, including both FI and PI instruments. The purpose of this volume is to provide information on output products expected from IDS investigators, required input data, and retrieval algorithms. Also included in this volume is the revised analysis of the 'best' and 'alternative' match data products for IDS input requirements. The original analysis presented in the August 1991 release of the SPSO Report was revised to incorporate the restructuring of the EOS platform. As a result of the reduced EOS payload, some of EOS instruments were deselected and their data products would not be available for IDS research. Information on these data products is also presented.

  3. Molecular characterization of Portuguese patients with mucopolysaccharidosis type II shows evidence that the IDS gene is prone to splicing mutations.

    PubMed

    Alves, S; Mangas, M; Prata, M J; Ribeiro, G; Lopes, L; Ribeiro, H; Pinto-Basto, J; Lima, M Reis; Lacerda, L

    2006-12-01

    Mucopolysaccharidosis type II (MPS II) is an X-linked recessive lysosomal storage disease caused by a defect in the iduronate-2-sulfatase gene (IDS). Alternative splicing of the IDS gene can occur and the underlying regulatory mechanism may be rather complex. Nevertheless, little information is available on the role of variations at the IDS locus in the splicing process. Here we report that splice mutations at the IDS locus are an important source of MPS II pathogenicity, accounting for almost 56% of Portuguese cases. Among 16 unrelated Portuguese MPS II patients, 15 different mutations were identified: six intronic splice mutations (c.104-2AG, c.241-2A>G, c.241-1G>A, c.418+1G>A, c.880-8AG and c.1181-1G>C); two exonic splice mutations (c.1006G>lC and c.1122C>T); five missense mutations (D269V, D69V, D148N, R88C and P86L); one nonsense mutation (Q465Ter); one total IDS gene deletion; and one rearrangement involving a IDS gene inversion. Furthermore, nine of the 15 detected mutations affected the usual splicing pattern at the locus. Some of them are responsible for dramatic changes in the splicing mechanism. For example, the substitution mutation, c.418+1G>A, revealed the presence of an exonic sequence inside intron 3. Our study provides evidence that the IDS locus is prone to splicing mutations and that such susceptibility is particularly high in exon 3 and neighbouring regions. Consequently, mutation screening of the IDS gene cannot be restricted to gDNA examination. Unless cDNA analysis is also conducted, misclassifications as silent or missense mutations can be produced and even uncharacteristic splice-site mutations can be misinterpreted as classic splicing defects that may generate severe, unconventional splicing alterations.

  4. Bypassing STAT3-mediated inhibition of the transcriptional regulator ID2 improves the antitumor efficacy of dendritic cells.

    PubMed

    Li, Haiyan S; Liu, Chengwen; Xiao, Yichuan; Chu, Fuliang; Liang, Xiaoxuan; Peng, Weiyi; Hu, Jianhua; Neelapu, Sattva S; Sun, Shao-Cong; Hwu, Patrick; Watowich, Stephanie S

    2016-01-01

    Despite the potent ability of dendritic cells (DCs) to stimulate lymphocyte responses and host immunity, granulocyte-macrophage colony-stimulating factor-derived DCs (GM-DCs) used as antitumor vaccines have demonstrated relatively modest success in cancer immunotherapy. We found that injecting GM-DCs into melanoma tumors in mice, or culturing GM-DCs with melanoma-secreted cytokines or melanoma-conditioned medium, rapidly suppressed DC-intrinsic expression of the gene encoding inhibitor of differentiation 2 (ID2), a transcriptional regulator. Melanoma-associated cytokines repressed Id2 transcription in murine DCs through the activation of signal transducer and activator of transcription 3 (STAT3). Enforced expression of ID2 in GM-DCs (ID2-GM-DCs) suppressed their production of the proinflammatory cytokine tumor necrosis factor-α (TNF-α). Vaccination with ID2-GM-DCs slowed the progression of melanoma tumors and enhanced animal survival, which was associated with an increased abundance of tumor-infiltrating interferon-γ-positive CD4(+) effector and CD8(+) cytotoxic T cells and a decreased number of tumor-infiltrating regulatory CD4(+) T cells. The efficacy of the ID2-GM-DC vaccine was improved by combinatorial treatment with a blocking antibody to programmed cell death protein-1 (PD-1), a current immunotherapy that overcomes suppressive immune checkpoint signaling. Collectively, our data reveal a previously unrecognized STAT3-mediated immunosuppressive mechanism in DCs and indicate that DC-intrinsic ID2 promotes tumor immunity by modulating tumor-associated CD4(+) T cell responses. Thus, inhibiting STAT3 or overexpressing ID2 selectively in DCs may improve the efficiency of DC vaccines in cancer therapy. PMID:27678219

  5. 7 CFR 7.11 - County committee members.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... the county executive director, other employee of the county committee, or the county agricultural extension agent for the county. If the county agricultural extension agent is not selected as secretary to... Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION...

  6. 7 CFR 7.11 - County committee members.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... the county executive director, other employee of the county committee, or the county agricultural extension agent for the county. If the county agricultural extension agent is not selected as secretary to... Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION...

  7. 7 CFR 7.11 - County committee members.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the county executive director, other employee of the county committee, or the county agricultural extension agent for the county. If the county agricultural extension agent is not selected as secretary to... Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION...

  8. 7 CFR 7.25 - County executive director duties.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false County executive director duties. 7.25 Section 7.25... CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES § 7.25 County executive director duties. (a) The county executive director shall execute the policies established by the county committee and be responsible for...

  9. 7 CFR 7.25 - County executive director duties.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false County executive director duties. 7.25 Section 7.25... CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES § 7.25 County executive director duties. (a) The county executive director shall execute the policies established by the county committee and be responsible for...

  10. 78 FR 10249 - Environmental Impact Statement: Will and Kankakee Counties, IL and Lake County, IN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-13

    ... TRANSPORTATION Federal Highway Administration Environmental Impact Statement: Will and Kankakee Counties, IL and Lake County, IN AGENCY: Federal Highway Administration (FHWA), DOT. ACTION: Notice of Intent. SUMMARY... Federal Domestic Assistance Program Number 20.205, Highway Research, Planning and Construction....

  11. Wide allelic heterogeneity with predominance of large IDS gene complex rearrangements in a sample of Mexican patients with Hunter syndrome.

    PubMed

    Alcántara-Ortigoza, M A; García-de Teresa, B; González-Del Angel, A; Berumen, J; Guardado-Estrada, M; Fernández-Hernández, L; Navarrete-Martínez, J I; Maza-Morales, M; Rius-Domínguez, R

    2016-05-01

    Hunter syndrome or mucopolysaccharidosis type II (MPSII) is caused by pathogenic variants in the IDS gene. This is the first study that examines the mutational spectrum in 25 unrelated Mexican MPSII families. The responsible genotype was identified in 96% of the families (24/25) with 10 novel pathogenic variants: c.133G>C, c.1003C>T, c.1025A>C, c.463_464delinsCCGTATAGCTGG, c.754_767del, c.1132_1133del, c.1463del, c.508-1G>C, c.1006+1G>T and c.(-217_103del). Extensive IDS gene deletions were identified in four patients; using DNA microarray analysis two patients showed the loss of the entire AFF2 gene, and epilepsy developed in only one of them. Wide allelic heterogeneity was noted, with large gene alterations (e.g. IDS/IDSP1 gene inversions, partial to extensive IDS deletions, and one chimeric IDS-IDSP1 allele) that occurred at higher frequencies than previously reported (36% vs 18.9-29%). The frequency of carrier mothers (80%) is consistent with previous descriptions (>70%). Carrier assignment allowed molecular prenatal diagnoses. Notably, somatic and germline mosaicism was identified in one family, and two patients presented thrombocytopenic purpura and pancytopenia after idursulfase enzyme replacement treatment. Our findings suggest a wide allelic heterogeneity in Mexican MPSII patients; DNA microarray analysis contributes to further delineation of the resulting phenotype for IDS and neighboring loci deletions.

  12. Wide allelic heterogeneity with predominance of large IDS gene complex rearrangements in a sample of Mexican patients with Hunter syndrome.

    PubMed

    Alcántara-Ortigoza, M A; García-de Teresa, B; González-Del Angel, A; Berumen, J; Guardado-Estrada, M; Fernández-Hernández, L; Navarrete-Martínez, J I; Maza-Morales, M; Rius-Domínguez, R

    2016-05-01

    Hunter syndrome or mucopolysaccharidosis type II (MPSII) is caused by pathogenic variants in the IDS gene. This is the first study that examines the mutational spectrum in 25 unrelated Mexican MPSII families. The responsible genotype was identified in 96% of the families (24/25) with 10 novel pathogenic variants: c.133G>C, c.1003C>T, c.1025A>C, c.463_464delinsCCGTATAGCTGG, c.754_767del, c.1132_1133del, c.1463del, c.508-1G>C, c.1006+1G>T and c.(-217_103del). Extensive IDS gene deletions were identified in four patients; using DNA microarray analysis two patients showed the loss of the entire AFF2 gene, and epilepsy developed in only one of them. Wide allelic heterogeneity was noted, with large gene alterations (e.g. IDS/IDSP1 gene inversions, partial to extensive IDS deletions, and one chimeric IDS-IDSP1 allele) that occurred at higher frequencies than previously reported (36% vs 18.9-29%). The frequency of carrier mothers (80%) is consistent with previous descriptions (>70%). Carrier assignment allowed molecular prenatal diagnoses. Notably, somatic and germline mosaicism was identified in one family, and two patients presented thrombocytopenic purpura and pancytopenia after idursulfase enzyme replacement treatment. Our findings suggest a wide allelic heterogeneity in Mexican MPSII patients; DNA microarray analysis contributes to further delineation of the resulting phenotype for IDS and neighboring loci deletions. PMID:26762690

  13. SpaceWire Protocol ID: What Does It Mean To You?

    NASA Technical Reports Server (NTRS)

    Rakow, Glenn; Schnurr, Richard; Gilley, Daniel; Parks, Steve

    2006-01-01

    packet format adds one new field to the packet that greatly enhances the capability of Spacewire. This new field called the Protocol Identifier (ID) is used to identify the packet contents and the associated processing for the packet. This feature along with the restriction in the packet format that uses the Protocol ID, allows a deterministic method of decoding packets that was not before possible. The first part of the packet is still the Destination Address, which still conforms to the original standard but with one restriction. The restriction is that the first byte seen at the destination by the user needs to be a logical address, independent of the addressing scheme used. The second field is defined as the Protocol ID, which is usually one byte in length. The packet cargo (user defined) follows the Protocol ID. After the packet cargo is the EOP, which defines the end of packet. The value of the Protocol ID is assigned by the Spacewire working group and the protocol description published for others to use. The development of Protocols for Spacewire is currently the area of greatest activity by the Spacewire working group. The first protocol definition by the working group has been completed and is now in the process of formal standardization. There are many other protocols in development for missions that have not yet received formal Protocol ID assignment, but even if the protocols are not formally assigned a value, this effort will provide synergism for future developments.

  14. Water resources of Lehigh County, Pennsylvania

    USGS Publications Warehouse

    Wood, Charles R.; Flippo, Herbert N.; Lescinsky, Joseph B.; Barker, James L.

    1972-01-01

    Lehigh County occupies an area of 347 square miles in southeastern Pennsylvania. The northern part of Lehigh County is underlain by the Martinsburg Formation, which consists chiefly of shale and slate. The central part of the county, where most of the population centers are located and much of the urbanization is occurring, is underlain by alternating beds of limestone and dolomite. From oldest to youngest, these carbonate rocks are the Leithsville Formation, the Allentown Formation, the Beekmantown Group, and the Jacksonburg Formation. The southern part of the county is underlain chiefly by the shales, sandstones, and conglomerates of the Brunswick Formation and by metamorphic and igneous rocks.

  15. Waste source reduction county government case study

    SciTech Connect

    1990-12-31

    Itasca County is located in north-central Minnesota, has a population of 42,000 and is known for its forests and scenic waterways. With Beltrami County, it contains the upper watershed of the Mississippi River. Its major industries are timber and tourism. Itasca County government made a commitment to source reduce its waste as much as possible. Secondarily, what they could not reduce they committed themselves to recycle. The project demonstrates functional reduction in practice. It shows that reduction is a realistic goal for county governments and that reduction can be measured on a waste stream by waste stream basis.

  16. 76 FR 56145 - Clearwater National Forest; ID; Upper Lochsa Land Exchange EIS

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-12

    ... Western Pacific Timber (WPT) located in Idaho and Clearwater Counties. The land is intermingled with the... drainage. For the past 50 years, WPT lands were managed primarily for timber production. For the most part these lands currently meet State Best Management Practices for timber production lands. The...

  17. Digital computer processing of LANDSAT data for North Alabama. [Linestone County, Madison County, Jackson County, Marshall County, and DeKalb County

    NASA Technical Reports Server (NTRS)

    Bond, A. D.; Atkinson, R. J.; Lybanon, M.; Ramapriyan, H. K.

    1977-01-01

    Computer processing procedures and programs applied to Multispectral Scanner data from LANDSAT are described. The output product produced is a level 1 land use map in conformance with a Universal Transverse Mercator projection. The region studied was a five-county area in north Alabama.

  18. Does extending the dual-task functional exercises workout improve postural balance in individuals with ID?

    PubMed

    Mikolajczyk, Edyta; Jankowicz-Szymanska, Agnieszka

    2015-03-01

    Maintaining postural balance, overcoming visual and motor coordination disorders and experiencing problems with low general fitness - typical of intellectually disabled individuals - adversely affect the performance quality of their activities of daily living (ADLs). Physical fitness and postural balance can be improved by taking part in special intervention programs. Our study was designed to test whether extending the dual-task intervention program (combining ADLs with balance exercises on unstable surfaces) from 12 to 24 weeks additionally improved postural balance in individuals with intellectual disability (ID). We also attempted to assess whether the effects of the above intervention program were still noticeable after 8 weeks of holidays, in which participants did not take any rehabilitation exercises. A total of 34 adolescents, aged 14-16 years (15.06±0.9), with moderate ID took part in our study. The experimental group (E) consisted of 17 individuals, who continued the intervention program originated 3 months earlier, and the control group (C) comprised the same number of participants. Postural balance was assessed on a stabilometric platform Alfa. Having extended the workout period by another 12 weeks, we noticed that the path length of the center of pressure (COP) covered by participants on tests with their eyes open and closed significantly shortened. After a lapse of 8 weeks from the completion of the program, the experimental group revealed a statistically significant decrease in the velocity along the medio-lateral (M/L) and anterior-posterior (A/P) axes. The remaining variables stayed at the same level and the control group did not demonstrate any statistically significant changes. Dual-task exercises, in which enhancing functional tasks of daily living is combined with a parallel stimulation of balance reactions, may improve static balance in persons with ID.

  19. The effect of IDS (immediate dentin sealing) on dentin bond strength under various thermocycling periods

    PubMed Central

    Leesungbok, Richard; Lee, Sang-Min; Park, Su-Jung; Lee, Suk-Won; Lee, Do Yun; Im, Byung-Jin

    2015-01-01

    PURPOSE The purpose of this study was to find out the effect of immediate dentin sealing (IDS) on bond strength of ceramic restoration under various thermocycling periods with DBA (dentin bonding agent system). MATERIALS AND METHODS Fifty freshly extracted human mandibular third molars were divided into 5 groups (1 control and 4 experimental groups) of 10 teeth. We removed enamel layer of sound teeth and embedded them which will proceed to be IDS, using All Bond II. A thermocycling was applied to experimental groups for 1, 2, 7, 14 days respectively and was not applied to control group. IPS Empress II for ceramic was acid-etched with ceramic etchant (9.5% HF) and silane was applied. Each ceramic disc was bonded to specimens with Duo-link, dual curable resin cement by means of light curing for 100 seconds. After the cementation procedures, shear bond strength measurement and SEM analysis of the fractured surface were done. The data were analyzed with a one-way ANOVA and Tukey multiple comparison test (α=.05). RESULTS There were no statistically significant differences between 4 experimental groups and control group, however the mean value started to decrease in group 7d, and group 14d showed the lowest mean bond strength in all groups. Also, group 7d and 14d showed distinct exposed dentin and collapsed hybrid layer was observed in SEM analysis. CONCLUSION In the present study, it can be concluded that ceramic restorations like a laminate veneer restoration should be bonded using resin cement within one week after IDS procedure. PMID:26140174

  20. ID-SERS Based Reference Method for Quantification of Large Biomolecules on a Single Chip

    SciTech Connect

    Yaghobian, Fatemeh; Stosch, Rainer; Henrion, Andre; Guettler, Bernd

    2010-08-06

    Accuracy and precision of quantitative SERS results have been significantly increased by applying a method based on the so-called isotope-dilution (ID) principle. In this ID-SERS approach, an isotopically labeled analogue of the target molecule (isotopologue) is spiked to the sample at a known concentration. Due to the slight difference in their molar masses, some Raman bands of the heavier isotopologue are red-shifted with respect to the same signals resulting from the unlabelled compound. As a result, spectra evaluation is reduced to the determination of intensity ratios rather than absolute intensities, and the unknown quantity of the analyte can be calculated from the known quantity of the standard. This procedure is of particular interest in the development of highly accurate reference procedures for metrology in chemistry. Because the sample is spiked prior to any further treatment, potential loss of material or matrix effects would equally affect both isotopologues, without influencing the final result. The method has been successfully applied for quantifying small diagnostic marker molecules like creatinine at their relevant serum concentration levels using silver colloids as SERS substrates. Now, the ID-SERS approach has been realized as a 'one-chip' approach using 'Bio-chips' made of intrinsically grown spherical silver nanoparticles with gaps less than 10 nm in between (Fig. 1). In addition, the scope of the method has been extended to larger biomolecules like peptides which will be shown using the example of the human growth-hormone (hGH) peptide T12 at physiologically relevant serum concentration levels (Fig. 2). Further developments towards the quantification of full proteins will also be reported.