Reconnaissance Report Section 107, Weeks Bay, Baldwin County, Alabama

DTIC Science & Technology

1990-08-01

Goals and Objectives 6 Management 7 Plan Formulation 7 Economic Analysis 7 Channel Design 9 Dredging Cost Computations 11 Cost-Benefit Analysis 11...Aerial Photography of Study Area 2 Channel Location and Reaches 3 LIST OF APPENDICES Title Appendix Pertinent Correspondence A Economic Analysis B... community situated on the north side of the Magnolia River approximately a mile and a half from the bay, the River Bluff subdivision, and the community of

Are restored side channels sustainable aquatic habitat features? Predicting the potential persistence of side channels as aquatic habitats based on their fine sedimentation dynamics

NASA Astrophysics Data System (ADS)

Riquier, Jérémie; Piégay, Hervé; Lamouroux, Nicolas; Vaudor, Lise

2017-10-01

The restoration of side channels (also referred to as abandoned channels, former channels, floodplain channels, or side arms) is increasingly implemented to improve the ecological integrity of river-floodplain systems. However, the design of side channel restoration projects remains poorly informed by theory or empirical observations despite the increasing number of projects. Moreover, feedback regarding the hydromorphological adjustment of restored channels is rarely documented, making it difficult to predict channel persistence as aquatic habitats. In this study, we analyze the spatial and temporal patterns of fine sediment deposition (< 2 mm) in 16 side channels of the Rhône River, France, restored in 1999-2006 by a combination of dredging and/or partial to full reconnection of their extremities and as a by-product of an increase in minimum flow through the bypassed main channels. We develop prediction tools to assess the persistence of restored channels as aquatic habitats, using between five and seven monitoring surveys per channel (spanning 7-15 years after restoration). Observed channel-averaged sedimentation rates ranged from 0 to 40.3 cm·y- 1 and reached 90.3 cm·y- 1 locally. Some channels exhibited a significant decline of sedimentation rates through time, whereas others maintained rather constant rates. Scouring processes (i.e., self-rejuvenation capacity) were occasionally documented in 15 channels. Six of the 16 studied channels appeared to be self-sustaining. The 10 others accumulated more and more fine sediment deposits after restoration. Parametric modeling of sedimentation rates suggested that among these 10 channels, four have long life-durations (i.e., more than a century), three have intermediate life-durations (i.e., likely between three and nine decades), and three others have short life-durations (i.e., likely between two and five decades). Observed channel-averaged sedimentation rates can be predicted from the frequency and magnitude (i.e., maximum shear stress) of upstream overflow events and the maximum intensity of backflow events (i.e., maximum backflow capacity). These predictors reflect the dominant role of side channel geometry (i.e., morphology of the upstream alluvial plug, slope conditions) in controlling their flooding regime. These models applied successfully to a wide range of channel morphologies and can be used to quantify a priori the likely effects and the sustainability of side channel restoration.

Regulating Effect of Asymmetrical Impeller on the Flow Distributions of Double-sided Centrifugal Compressor

NASA Astrophysics Data System (ADS)

Yang, Ce; Liu, Yixiong; Yang, Dengfeng; Wang, Benjiang

2017-11-01

To achieve the rebalance of flow distributions of double-sided impellers, a method of improving the radius of rear impeller is presented in this paper. It is found that the flow distributions of front and rear impeller can be adjusted effectively by increasing the radius of rear impeller, thus improves the balance of flow distributions of front and rear impeller. Meanwhile, the working conversion mode process of double-sided centrifugal compressor is also changed. Further analysis shows that the flowrates of blade channels in front impeller are mainly influenced by the circumferential distributions of static pressure in the volute. But the flowrates of rear impeller blade channels are influenced by the outlet flow field of bent duct besides the effects of static pressure distributions in the volute. In the airflow interaction area downstream, the flowrate of blade channel is obviously smaller. By increasing the radius of rear impeller, the work capacity of rear impeller is enhanced, the working mode conversion process from parallel working mode of double-sided impeller to the single impeller working mode is delayed, and the stable working range of double-sided compressor is broadened.

Enhancing Electromagnetic Side-Channel Analysis in an Operational Environment

NASA Astrophysics Data System (ADS)

Montminy, David P.

Side-channel attacks exploit the unintentional emissions from cryptographic devices to determine the secret encryption key. This research identifies methods to make attacks demonstrated in an academic environment more operationally relevant. Algebraic cryptanalysis is used to reconcile redundant information extracted from side-channel attacks on the AES key schedule. A novel thresholding technique is used to select key byte guesses for a satisfiability solver resulting in a 97.5% success rate despite failing for 100% of attacks using standard methods. Two techniques are developed to compensate for differences in emissions from training and test devices dramatically improving the effectiveness of cross device template attacks. Mean and variance normalization improves same part number attack success rates from 65.1% to 100%, and increases the number of locations an attack can be performed by 226%. When normalization is combined with a novel technique to identify and filter signals in collected traces not related to the encryption operation, the number of traces required to perform a successful attack is reduced by 85.8% on average. Finally, software-defined radios are shown to be an effective low-cost method for collecting side-channel emissions in real-time, eliminating the need to modify or profile the target encryption device to gain precise timing information.

Side Channels of the Impounded and Middle Mississippi River: Opportunities and Challenges to Maximize Restoration Potential

DTIC Science & Technology

2016-07-01

considered. That is, it may be necessary to renew side channels according to a schedule to prevent them from filling with sediment and becoming terrestrial...maintain side channels as a restoration feature or to control their succession (i.e., prevent their loss through sedimentation ) have not been developed...features for maximum benefit? • How could advantage be taken of the existing sediment transport regime and flow pattern to maintain side channels or

An analysis of induced pressure fields in electroosmotic flows through microchannels.

PubMed

Zhang, Yonghao; Gu, Xiao-Jun; Barber, Robert W; Emerson, David R

2004-07-15

Induced pressure gradients are found to cause band-broadening effects which are important to the performance of microfluidic devices, such as capillary electrophoresis and capillary chromatography. An improved understanding of the underlying mechanisms causing an induced pressure gradient in electroosmotic flows is presented. The analysis shows that the induced pressure distribution is the key to understanding the experimentally observed phenomena of leakage flows. A novel way of determining the static pressures at the inlet and outlet of microchannels is also presented that takes account of the pressure losses due to flow contraction and expansion. These commonly neglected pressure losses at the channel entrance and outlet are shown to be important in accurately describing the flow. The important parameters that define the effect of induced pressure on the flows are discussed, which may facilitate the design of improved microfluidic devices. The present model clearly identifies the mechanism behind the experimentally observed leakage flows, which is further confirmed by numerical simulations. Not only can the leakage flow occur from the electric-field-free side channel to the main channel, but also the fluid in the main channel can be attracted into the side channel by the induced pressure gradient. Copyright 2004 Elsevier Inc.

Flow boiling with enhancement devices for cold plate coolant channel design

NASA Technical Reports Server (NTRS)

Boyd, Ronald D.; Turknett, Jerry C.; Smith, Alvin

1989-01-01

The effects of enhancement devices on flow boiling heat transfer in circular coolant channels, which are heated over a fraction of their perimeters, are studied. The variations were examined in both the mean and local (axial, and circumferential) heat transfer coefficients for a circular coolant channel with either smooth walls or with both a twisted tape and spiral finned walls. Improvements were initiated in the present data reduction analysis. These efforts should lead to the development of heat transfer correlations which include effects of single side heat flux and enhancement device configuration. It is hoped that a stage will be set for the study of heat transfer and pressure drop in single sided heated systems under zero gravity conditions.

Comparison of High Aspect Ratio Cooling Channel Designs for a Rocket Combustion Chamber with Development of an Optimized Design

NASA Technical Reports Server (NTRS)

Wadel, Mary F.

1998-01-01

An analytical investigation on the effect of high aspect ratio (height/width) cooling channels, considering different coolant channel designs, on hot-gas-side wall temperature and coolant pressure drop for a liquid hydrogen cooled rocket combustion chamber, was performed. Coolant channel design elements considered were: length of combustion chamber in which high aspect ratio cooling was applied, number of coolant channels, and coolant channel shape. Seven coolant channel designs were investigated using a coupling of the Rocket Thermal Evaluation code and the Two-Dimensional Kinetics code. Initially, each coolant channel design was developed, without consideration for fabrication, to reduce the hot-gas-side wall temperature from a given conventional cooling channel baseline. These designs produced hot-gas-side wall temperature reductions up to 22 percent, with coolant pressure drop increases as low as 7.5 percent from the baseline. Fabrication constraints for milled channels were applied to the seven designs. These produced hot-gas-side wall temperature reductions of up to 20 percent, with coolant pressure drop increases as low as 2 percent. Using high aspect ratio cooling channels for the entire length of the combustion chamber had no additional benefit on hot-gas-side wall temperature over using high aspect ratio cooling channels only in the throat region, but increased coolant pressure drop 33 percent. Independent of coolant channel shape, high aspect ratio cooling was able to reduce the hot-gas-side wall temperature by at least 8 percent, with as low as a 2 percent increase in coolant pressure drop. ne design with the highest overall benefit to hot-gas-side wall temperature and minimal coolant pressure drop increase was the design which used bifurcated cooling channels and high aspect ratio cooling in the throat region. An optimized bifurcated high aspect ratio cooling channel design was developed which reduced the hot-gas-side wall temperature by 18 percent and reduced the coolant pressure drop by 4 percent. Reductions of coolant mass flow rate of up to 50 percent were possible before the hot-gas-side wall temperature reached that of the baseline. These mass flow rate reductions produced coolant pressure drops of up to 57 percent.

Forced convection and flow boiling with and without enhancement devices for top-side-heated horizontal channels

NASA Technical Reports Server (NTRS)

Boyd, Ronald D., Sr.; Turknett, Jerry C.

1989-01-01

The effect of enhancement devices on flow boiling heat transfer in coolant channels, which are heated either from the top side or uniformly was studied. Studies are completed of the variations in the local (axial and circumferential) and mean heat transfer coefficients in horizontal, top-heated coolant channels with smooth walls and internal heat transfer enhancement devices. The working fluid is freon-11. The objectives are to: (1) examine the variations in both the mean and local (axial and circumferential) heat transfer coefficients for a circular coolant channel with either smooth walls or with both a twisted tape and spiral finned walls; (2) examine the effect of channel diameter (and the length-to-diameter aspect ratio) variations for the smooth wall channel; and (3) develop and improved data reduction analysis. The case of the top-heated, horizontal flow channel with smooth wall (1.37 cm inside diameter, and 122 cm heated length) was completed. The data were reduced using a preliminary analysis based on the heated hydraulic diameter. Preliminary examination of the local heat transfer coefficient variations indicated that there are significant axial and circumferential variations. However, it appears that the circumferential variation is more significant than the axial ones. In some cases, the circumferential variations were as much as a factor of ten. The axial variations rarely exceeded a factor of three.

The effect of the amino-acid side chains on the energy profiles for ion transport in the gramicidin A channel.

PubMed

Etchebest, C; Pullman, A

1985-02-01

Computations on the energy profiles for Na+ in the gramicidin A (GA) channel have been extended by introducing the effect, previously neglected, of the amino acid side chains of GA, fixed in their most stable conformations. The calculations have been performed in two approximations: 1) with the ethanolamine tail fixed in its most stable conformation, 2) with the tail allowed to optimize its conformation upon the progression of the ion. In both approximations the overall shape of the energy profile is very similar to that obtained in the absence of the side chains. One observes, however, a general lowering of the profile upon the adjunction of the side chains. The analysis of the factors responsible for this energy lowering indicates that it is due essentially to the electrostatic and polarisation components of the interaction which interplay differently, however, in the different parts of the channel. A particular role is attributed in this respect to the tryptophan residues of GA. The role of the 4 tryptophans present, Trp 15, 13, 11 and 9, is individualized by stripping of one of them at a time. The strongest effect on the energy deepening is due to Trp 13 and is particularly prominent in the entrance zone at 14.5A from the center of the channel. The result indicates the possibility of investigating theoretically the effect on the energy profiles of the substitution of the "natural" side chain by others.

Radial flow nuclear thermal rocket (RFNTR)

DOEpatents

Leyse, Carl F.

1995-11-07

A radial flow nuclear thermal rocket fuel assembly includes a substantially conical fuel element having an inlet side and an outlet side. An annular channel is disposed in the element for receiving a nuclear propellant, and a second, conical, channel is disposed in the element for discharging the propellant. The first channel is located radially outward from the second channel, and separated from the second channel by an annular fuel bed volume. This fuel bed volume can include a packed bed of loose fuel beads confined by a cold porous inlet frit and a hot porous exit frit. The loose fuel beads include ZrC coated ZrC-UC beads. In this manner, nuclear propellant enters the fuel assembly axially into the first channel at the inlet side of the element, flows axially across the fuel bed volume, and is discharged from the assembly by flowing radially outward from the second channel at the outlet side of the element.

Radial flow nuclear thermal rocket (RFNTR)

DOEpatents

Leyse, Carl F.

1995-01-01

A radial flow nuclear thermal rocket fuel assembly includes a substantially conical fuel element having an inlet side and an outlet side. An annular channel is disposed in the element for receiving a nuclear propellant, and a second, conical, channel is disposed in the element for discharging the propellant. The first channel is located radially outward from the second channel, and separated from the second channel by an annular fuel bed volume. This fuel bed volume can include a packed bed of loose fuel beads confined by a cold porous inlet frit and a hot porous exit frit. The loose fuel beads include ZrC coated ZrC-UC beads. In this manner, nuclear propellant enters the fuel assembly axially into the first channel at the inlet side of the element, flows axially across the fuel bed volume, and is discharged from the assembly by flowing radially outward from the second channel at the outlet side of the element.

Planar ceramic membrane assembly and oxidation reactor system

DOEpatents

Carolan, Michael Francis; Dyer, legal representative, Kathryn Beverly; Wilson, Merrill Anderson; Ohm, Ted R.; Kneidel, Kurt E.; Peterson, David; Chen, Christopher M.; Rackers, Keith Gerard; Dyer, deceased, Paul Nigel

2007-10-09

Planar ceramic membrane assembly comprising a dense layer of mixed-conducting multi-component metal oxide material, wherein the dense layer has a first side and a second side, a porous layer of mixed-conducting multi-component metal oxide material in contact with the first side of the dense layer, and a ceramic channeled support layer in contact with the second side of the dense layer. The planar ceramic membrane assembly can be used in a ceramic wafer assembly comprising a planar ceramic channeled support layer having a first side and a second side; a first dense layer of mixed-conducting multi-component metal oxide material having an inner side and an outer side, wherein the inner side is in contact with the first side of the ceramic channeled support layer; a first outer support layer comprising porous mixed-conducting multi-component metal oxide material and having an inner side and an outer side, wherein the inner side is in contact with the outer side of the first dense layer; a second dense layer of mixed-conducting multi-component metal oxide material having an inner side and an outer side, wherein the inner side is in contact with the second side of the ceramic channeled layer; and a second outer support layer comprising porous mixed-conducting multi-component metal oxide material and having an inner side and an outer side, wherein the inner side is in contact with the outer side of the second dense layer.

Planar ceramic membrane assembly and oxidation reactor system

DOEpatents

Carolan, Michael Francis; Dyer, legal representative, Kathryn Beverly; Wilson, Merrill Anderson; Ohrn, Ted R.; Kneidel, Kurt E.; Peterson, David; Chen, Christopher M.; Rackers, Keith Gerard; Dyer, Paul Nigel

2009-04-07

Planar ceramic membrane assembly comprising a dense layer of mixed-conducting multi-component metal oxide material, wherein the dense layer has a first side and a second side, a porous layer of mixed-conducting multi-component metal oxide material in contact with the first side of the dense layer, and a ceramic channeled support layer in contact with the second side of the dense layer. The planar ceramic membrane assembly can be used in a ceramic wafer assembly comprising a planar ceramic channeled support layer having a first side and a second side; a first dense layer of mixed-conducting multi-component metal oxide material having an inner side and an outer side, wherein the inner side is in contact with the first side of the ceramic channeled support layer; a first outer support layer comprising porous mixed-conducting multi-component metal oxide material and having an inner side and an outer side, wherein the inner side is in contact with the outer side of the first dense layer; a second dense layer of mixed-conducting multi-component metal oxide material having an inner side and an outer side, wherein the inner side is in contact with the second side of the ceramic channeled layer; and a second outer support layer comprising porous mixed-conducting multi-component metal oxide material and having an inner side and an outer side, wherein the inner side is in contact with the outer side of the second dense layer.

Bandwidth and Detection of Packet Length Covert Channels

DTIC Science & Technology

2011-03-01

Shared Resource Matrix ( SRM ): Develop a matrix of all resources on one side and on the other all the processes. Then, determine which process uses which...system calls. This method is similar to that of the SRM . Covert channels have also been created by modulating packet timing, data and headers of net- work...analysis, noninterference analysis, SRM method, and the covert flow tree method [4]. These methods can be used during the design phase of a system. Less

Basic metabolic panel

MedlinePlus

SMAC7; Sequential multi-channel analysis with computer-7; SMA7; Metabolic panel 7; CHEM-7 ... breathing problems, diabetes or diabetes-related complications, and medicine side effects. Talk to your provider about the ...

Thin walled channel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Crowther, R.L.; Johansson, E.B.

1988-06-07

A fuel assembly is described comprising fuel rods positioned in a spaced array by upper and lower tie-plates, an open ended flow channel surrounding the array for conducting coolant upward about the fuel rods, the open ended channel having a polygon shaped cross section with flat side sections connected between the corner sections; means separate from the channel connecting the upper and lower tie-plates together and maintaining the fuel rods in spaced array independent of the flow channel, improvement in the flow channel comprising: four corners having a first thickness; four sides having a second and reduced thickness from themore » corner thickness, the sides welded to the corner sections.« less

Secondary Channel Bifurcation Geometry: A Multi-dimensional Problem

NASA Astrophysics Data System (ADS)

Gaeuman, D.; Stewart, R. L.

2017-12-01

The construction of secondary channels (or side channels) is a popular strategy for increasing aquatic habitat complexity in managed rivers. Such channels, however, frequently experience aggradation that prevents surface water from entering the side channels near their bifurcation points during periods of relatively low discharge. This failure to maintain an uninterrupted surface water connection with the main channel can reduce the habitat value of side channels for fish species that prefer lotic conditions. Various factors have been proposed as potential controls on the fate of side channels, including water surface slope differences between the main and secondary channels, the presence of main channel secondary circulation, transverse bed slopes, and bifurcation angle. A quantitative assessment of more than 50 natural and constructed secondary channels in the Trinity River of northern California indicates that bifurcations can assume a variety of configurations that are formed by different processes and whose longevity is governed by different sets of factors. Moreover, factors such as bifurcation angle and water surface slope vary with discharge level and are continuously distributed in space, such that they must be viewed as a multi-dimensional field rather than a single-valued attribute that can be assigned to a particular bifurcation.

Autoradiographic localization of voltage-dependent sodium channels on the mouse neuromuscular junction using /sup 125/I-alpha scorpion toxin. I. Preferential labeling of glial cells on the presynaptic side

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boudier, J.L.; Jover, E.; Cau, P.

1988-05-01

Alpha-scorpion toxins bind specifically to the voltage-sensitive sodium channel in excitable membranes, and binding is potential-dependent. The radioiodinated toxin II from the scorpion Androctonus australis Hector (alpha ScTx) was used to localize voltage-sensitive sodium channels on the presynaptic side of mouse neuromuscular junctions (NMJ) by autoradiography using both light and electron microscopy. Silver grain localization was analyzed by the cross-fire method. At the light-microscopic level, grain density over NMJ appeared 6-8x higher than over nonjunctional muscle membrane. The specificity of labeling was verified by competition/displacement with an excess of native alpha ScTx. Labeling was also inhibited by incubation in depolarizingmore » conditions, showing its potential-dependence. At the electron-microscopic level, analysis showed that voltage-sensitive sodium channels labeled with alpha ScTx were almost exclusively localized on membranes, as expected. Due to washout after incubation, appreciable numbers of binding sites were not found on the postsynaptic membranes. However, on the presynaptic side, alpha ScTx-labeled voltage-sensitive sodium channels were localized on the membrane of non-myelin-forming Schwann cells covering NMJ. The axonal presynaptic membrane was not labeled. These results show that voltage-sensitive sodium channels are present on glial cells in vivo, as already demonstrated in vitro. It is proposed that these glial channels could be indirectly involved in the ionic homeostasis of the axonal environment.« less

Separation system with a sheath-flow supported electrochemical detector

DOEpatents

Mathies, Richard A [Moraga, CA; Emrich, Charles A [Berkeley, CA; Singhal, Pankaj [Pasadena, CA; Ertl, Peter [Styria, AT

2008-10-21

An electrochemical detector including side channels associated with a separation channel of a sample component separation apparatus is provided. The side channels of the detector, in one configuration, provide a sheath-flow for an analyte exiting the separation channel which directs the analyte to the electrically developed electrochemical detector.

Flow boiling with enhancement devices for cold plate coolant channel design

NASA Technical Reports Server (NTRS)

Boyd, Ronald D., Sr.

1989-01-01

A research program to study the effect of enhancement devices on flow boiling heat transfer in coolant channels, which are heated either from the top side or uniformly, is discussed. Freon 11 is the working fluid involved. The specific objectives are: (1) examine the variations in both the mean and local (axial and circumferential) heat transfer coefficients for a circular coolant channel with either smooth walls or with both a twisted tape and spiral finned walls, (2) examine the effect channel diameter (and the length-to-diameter aspect ratio) variations for the smooth wall channel, and (3) develop an improved data reduction analysis.

Physical Hydraulic Model of Side-Channel Spillway of Lambuk DAM, Bali

NASA Astrophysics Data System (ADS)

Harifa, A. C.; Sholichin, M.; Othman, F. B.

2013-12-01

The spillway is among the most important structures of a dam project. A spillway is designed to prevent overtopping of a dam at a place that is not designed for overtopping. Side-channel spillways are commonly used to release water flow from a reservoir in places where the sides are steep and have a considerable height above the dam. Experimental results were collected with a hydraulic model of the side-channel spillway for releasing the peak overflow of Lambuk Dam. This dam is, located on the Lambuk River, which is a tributary of the Yeh Hoo River ~ 34.6 km north of Denpasar on the island of Bali. The bituminous geomembrane faced dam is 24 m in height, with a 35-m wide spillway. The length of the side channel is 35 m long, with 58 m of transition channel, 67.37 m of chuteway channel and 22.71 m of stilling basin. The capacity of the spillway is 231.91 m3/s and the outlet works capacity is 165.28 m3/s. The reservoir is designed for irrigation and water supply. The purpose of this study was to optimize the designed of the structure and to ensure its safe operation. In hydraulic model may help the decision-makers to visualize the flow field before selecting a ';suitable' design. The hydraulic model study was performed to ensure passage of the maximum discharge at maximum reservoir capacity; to study the spillway approach conditions, water surface profiles, and flow patterns in the chuteway; and to reveal potential demerits of the proposed hydraulic design of various structures and explore solutions. The model was constructed at 1 : 40 scale, Reservoir topography was modeled using concrete, the river bed using sand and some gravel, the river berm using concrete, and the spillway and channel using Plexiglas. Water was measured using Rectangular contracted weir. Design floods (with return period in year) were Q2 = 111.40 m3/s, Q5 = 136.84 m3/s, Q10 = 159.32 m3/s, Q25 = 174.61 m3/s, Q50 = 185.13 m3/s, Q100 = 198.08 m3/s, Q200 = 210.55 m3/s, Q1000 = 231.91 m3/s and the probable maximum flood was 476.88 m3/s. Hydraulic analysis of spillway used USBR method for spillway, Hind's equation for the side channel, energy equation with standard step method for the transition and chuteway channel. Local scouring depth was calculated using the Schotlisch and Veronise equation. Total head on crest spillway for Q2 = 0.92 m, Q1000 = 1.68 m and for QPMF = 1.92 m. The highest measurement error is 3.16% according to the total head on crest spillway. Cavitation was observed in chuteway. Flow is subcritical (Froude < 1) in the side channel and supercritical in the transition channel. The final design for the spillway and chuteway were safe from impact of cavitation, pulsating flow, and local scouring.

Physical habitat dynamics in four side-channel chutes, lower Missouri River

USGS Publications Warehouse

Jacobson, Robert B.; Johnson, Harold E.; Laustrup, Mark S.; D'Urso, Gary J.; Reuter, Joanna M.

2004-01-01

Construction of the side-channel chutes has become a popular means to rehabilitate habitate of the Lower Missouri River. We studied various aspects of hydrology, hydraulics, and geomorphology of four side-channel chutes to document a range of existing conditions in the Lower Missouri River. The Cranberry Bend side-channel chute has existed for at least 40 years and is an example of a persistent, minimally engineered chute. The Lisbon Bottom side-channel chute is a young chute, created by extreme floods during 1993-1996, and allowed to evolve with minimum engineering of inlet and outlet structures. The Hamburg Bend and North Overton Bottom side-channel chutes were constructed in 1996 and 2000, respectively, as part of the Missouri River Bank Stabilization and navigation Fish and Wildlife Mitigation Project. These side-channel chutes provide increased areas of sandbars and shallow, slow water -- habitats thought to be substantially diminished in the modern Missouri River. Depths and velocities measured in side-channel chutes are also present in the main channel, but the chutes provide more areas of slow, shallow water and they increase the range of discharges over which shallow, slow water is present. The 3.6 km long Lisbon Bottom chute provides as much as 50% of the entire shallow water habitat that exists in the encompassing 15 km reach of the river. At Cranberry Bend and Lisbon Bottom, the side-channel chutes provided 10-40% of the availabile sandbar area in the reach, depending on discharge. Each of the side-channel chutes shows evidence of continuing erosion and deposition. The longevity and the Cranberry Bend chute attests to dynamic stability -- that is, a chute that maintains form and processes while shifting in position. The Hamburg chute similarly shows evidence of lateral movement and construction of flood plain to compensate for erosion. The Lisbon Bottom chute -- the most intensively studied chute -- appears to have achieved an equilibrium width and continues to migrate slowly; however, evidence of aggradation indicates that the chute has not reached an ultimate form, and may be continuing to adjust to altered hydrology and sediment availability. The North Overton Bottoms chute is the newest in the study. In its originally constructed form, the North Overton Bottoms pilot chute was extremely stable, even while being subjected to two floods in excess of 2-year recurrence interval and after accumulating large, potentially destabilizing large woody debris jams. Ongoing adaptive re-engineering of the North Overton Bottoms chute has prevented assessment of how the chute might have adjusted its form in the absence of intervention.

33 CFR 66.10-15 - Aids to navigation.

Code of Federal Regulations, 2012 CFR

2012-07-01

... solid colored buoy. A buoy which marks the left side of the channel viewed looking upstream or toward... channel, solid colored buoys shall be established in pairs, one on each side of the navigable channel... that the user should pass between the buoys. (c) On an irregularly defined channel, solid colored buoys...

33 CFR 66.10-15 - Aids to navigation.

Code of Federal Regulations, 2013 CFR

2013-07-01

... solid colored buoy. A buoy which marks the left side of the channel viewed looking upstream or toward... channel, solid colored buoys shall be established in pairs, one on each side of the navigable channel... that the user should pass between the buoys. (c) On an irregularly defined channel, solid colored buoys...

33 CFR 66.10-15 - Aids to navigation.

Code of Federal Regulations, 2014 CFR

2014-07-01

... solid colored buoy. A buoy which marks the left side of the channel viewed looking upstream or toward... channel, solid colored buoys shall be established in pairs, one on each side of the navigable channel... that the user should pass between the buoys. (c) On an irregularly defined channel, solid colored buoys...

Critical side channel effects in random bit generation with multiple semiconductor lasers in a polarization-based quantum key distribution system.

PubMed

Ko, Heasin; Choi, Byung-Seok; Choe, Joong-Seon; Kim, Kap-Joong; Kim, Jong-Hoi; Youn, Chun Ju

2017-08-21

Most polarization-based BB84 quantum key distribution (QKD) systems utilize multiple lasers to generate one of four polarization quantum states randomly. However, random bit generation with multiple lasers can potentially open critical side channels that significantly endangers the security of QKD systems. In this paper, we show unnoticed side channels of temporal disparity and intensity fluctuation, which possibly exist in the operation of multiple semiconductor laser diodes. Experimental results show that the side channels can enormously degrade security performance of QKD systems. An important system issue for the improvement of quantum bit error rate (QBER) related with laser driving condition is further addressed with experimental results.

Side-channel-free quantum key distribution.

PubMed

Braunstein, Samuel L; Pirandola, Stefano

2012-03-30

Quantum key distribution (QKD) offers the promise of absolutely secure communications. However, proofs of absolute security often assume perfect implementation from theory to experiment. Thus, existing systems may be prone to insidious side-channel attacks that rely on flaws in experimental implementation. Here we replace all real channels with virtual channels in a QKD protocol, making the relevant detectors and settings inside private spaces inaccessible while simultaneously acting as a Hilbert space filter to eliminate side-channel attacks. By using a quantum memory we find that we are able to bound the secret-key rate below by the entanglement-distillation rate computed over the distributed states.

Industrial stator vane with sequential impingement cooling inserts

DOEpatents

Jones, Russell B; Fedock, John A; Goebel, Gloria E; Krueger, Judson J; Rawlings, Christopher K; Memmen, Robert L

2013-08-06

A turbine stator vane for an industrial engine, the vane having two impingement cooling inserts that produce a series of impingement cooling from the pressure side to the suction side of the vane walls. Each insert includes a spar with a row of alternating impingement cooling channels and return air channels extending in a radial direction. Impingement cooling plates cover the two sides of the insert and having rows of impingement cooling holes aligned with the impingement cooling channels and return air openings aligned with the return air channel.

Tl+-induced micros gating of current indicates instability of the MaxiK selectivity filter as caused by ion/pore interaction.

PubMed

Schroeder, Indra; Hansen, Ulf-Peter

2008-04-01

Patch clamp experiments on single MaxiK channels expressed in HEK293 cells were performed at high temporal resolution (50-kHz filter) in asymmetrical solutions containing 0, 25, 50, or 150 mM Tl+ on the luminal or cytosolic side with [K+] + [Tl+] = 150 mM and 150 mM K+ on the other side. Outward current in the presence of cytosolic Tl+ did not show fast gating behavior that was significantly different from that in the absence of Tl+. With luminal Tl+ and at membrane potentials more negative than -40 mV, the single-channel current showed a negative slope resistance concomitantly with a flickery block, resulting in an artificially reduced apparent single-channel current I(app). The analysis of the amplitude histograms by beta distributions enabled the estimation of the true single-channel current and the determination of the rate constants of a simple two-state O-C Markov model for the gating in the bursts. The voltage dependence of the gating ratio R = I(true)/I(app) = (k(CO) + k(OC))/k(CO) could be described by exponential functions with different characteristic voltages above or below 50 mM Tl(+). The true single-channel current I(true) decreased with Tl+ concentrations up to 50 mM and stayed constant thereafter. Different models were considered. The most likely ones related the exponential increase of the gating ratio to ion depletion at the luminal side of the selectivity filter, whereas the influence of [Tl+] on the characteristic voltage of these exponential functions and of the value of I(true) were determined by [Tl+] at the inner side of the selectivity filter or in the cavity.

EMG-Torque Relation in Chronic Stroke: A Novel EMG Complexity Representation With a Linear Electrode Array.

PubMed

Zhang, Xu; Wang, Dongqing; Yu, Zaiyang; Chen, Xiang; Li, Sheng; Zhou, Ping

2017-11-01

This study examines the electromyogram (EMG)-torque relation for chronic stroke survivors using a novel EMG complexity representation. Ten stroke subjects performed a series of submaximal isometric elbow flexion tasks using their affected and contralateral arms, respectively, while a 20-channel linear electrode array was used to record surface EMG from the biceps brachii muscles. The sample entropy (SampEn) of surface EMG signals was calculated with both global and local tolerance schemes. A regression analysis was performed between SampEn of each channel's surface EMG and elbow flexion torque. It was found that a linear regression can be used to well describe the relation between surface EMG SampEn and the torque. Each channel's root mean square (RMS) amplitude of surface EMG signal in the different torque level was computed to determine the channel with the highest EMG amplitude. The slope of the regression (observed from the channel with the highest EMG amplitude) was smaller on the impaired side than on the nonimpaired side in 8 of the 10 subjects, regardless of the tolerance scheme (global or local) and the range of torques (full or matched range) used for comparison. The surface EMG signals from the channels above the estimated muscle innervation zones demonstrated significantly lower levels of complexity compared with other channels between innervation zones and muscle tendons. The study provides a novel point of view of the EMG-torque relation in the complexity domain, and reveals its alterations post stroke, which are associated with complex neural and muscular changes post stroke. The slope difference between channels with regard to innervation zones also confirms the relevance of electrode position in surface EMG analysis.

Continuous-variable quantum key distribution with a leakage from state preparation

NASA Astrophysics Data System (ADS)

Derkach, Ivan; Usenko, Vladyslav C.; Filip, Radim

2017-12-01

We address side-channel leakage in a trusted preparation station of continuous-variable quantum key distribution with coherent and squeezed states. We consider two different scenarios: multimode Gaussian modulation, directly accessible to an eavesdropper, or side-channel loss of the signal states prior to the modulation stage. We show the negative impact of excessive modulation on both the coherent- and squeezed-state protocols. The impact is more pronounced for squeezed-state protocols and may require optimization of squeezing in the case of noisy quantum channels. Further, we demonstrate that the coherent-state protocol is immune to side-channel signal state leakage prior to modulation, while the squeezed-state protocol is vulnerable to such attacks, becoming more sensitive to the noise in the channel. In the general case of noisy quantum channels the signal squeezing can be optimized to provide best performance of the protocol in the presence of side-channel leakage prior to modulation. Our results demonstrate that leakage from the trusted source in continuous-variable quantum key distribution should not be underestimated and squeezing optimization is needed to overcome coherent state protocols.

Side Channel Attacks on STTRAM and Low Overhead Countermeasures

DTIC Science & Technology

2017-03-20

introduce security vulnerabilities and expose the cache memory to side channel attacks. In this paper, we propose a side channel attack (SCA) model...where the adversary can monitor the supply current of the memory array to partially identify the sensi- tive cache data that is being read or written. We...propose solutions such as short retention STTRAM, obfuscation of SCA using 1-bit parity, multi-bit random write, and, neutral- izing the SCA using

Sequential cooling insert for turbine stator vane

DOEpatents

Jones, Russell B.; Krueger, Judson J.; Plank, William L.

2014-04-01

A sequential impingement cooling insert for a turbine stator vane that forms a double impingement for the pressure and suction sides of the vane or a triple impingement. The insert is formed from a sheet metal formed in a zigzag shape that forms a series of alternating impingement cooling channels with return air channels, where pressure side and suction side impingement cooling plates are secured over the zigzag shaped main piece. Another embodiment includes the insert formed from one or two blocks of material in which the impingement channels and return air channels are machined into each block.

Sequential cooling insert for turbine stator vane

DOEpatents

Jones, Russel B; Krueger, Judson J; Plank, William L

2014-11-04

A sequential impingement cooling insert for a turbine stator vane that forms a double impingement for the pressure and suction sides of the vane or a triple impingement. The insert is formed from a sheet metal formed in a zigzag shape that forms a series of alternating impingement cooling channels with return air channels, where pressure side and suction side impingement cooling plates are secured over the zigzag shaped main piece. Another embodiment includes the insert formed from one or two blocks of material in which the impingement channels and return air channels are machined into each block.

Hydrologic considerations for estimation of storage-capacity requirements of impounding and side-channel reservoirs for water supply in Ohio

USGS Publications Warehouse

Koltun, G.F.

2001-01-01

This report provides data and methods to aid in the hydrologic design or evaluation of impounding reservoirs and side-channel reservoirs used for water supply in Ohio. Data from 117 streamflow-gaging stations throughout Ohio were analyzed by means of nonsequential-mass-curve-analysis techniques to develop relations between storage requirements, water demand, duration, and frequency. Information also is provided on minimum runoff for selected durations and frequencies. Systematic record lengths for the streamflow-gaging stations ranged from about 10 to 75 years; however, in many cases, additional streamflow record was synthesized. For impounding reservoirs, families of curves are provided to facilitate the estimation of storage requirements as a function of demand and the ratio of the 7-day, 2-year low flow to the mean annual flow. Information is provided with which to evaluate separately the effects of evaporation on storage requirements. Comparisons of storage requirements for impounding reservoirs determined by nonsequential-mass-curve-analysis techniques with storage requirements determined by annual-mass-curve techniques that employ probability routing to account for carryover-storage requirements indicate that large differences in computed required storages can result from the two methods, particularly for conditions where demand cannot be met from within-year storage. For side-channel reservoirs, tables of demand-storage-frequency information are provided for a primary pump relation consisting of one variable-speed pump with a pumping capacity that ranges from 0.1 to 20 times demand. Tables of adjustment ratios are provided to facilitate determination of storage requirements for 19 other pump sets consisting of assorted combinations of fixed-speed pumps or variable-speed pumps with aggregate pumping capacities smaller than or equal to the primary pump relation. The effects of evaporation on side-channel reservoir storage requirements are incorporated into the storage-requirement estimates. The effects of an instream-flow requirement equal to the 80-percent-duration flow are also incorporated into the storage-requirement estimates.

μPIV measurements of two-phase flows of an operated direct methanol fuel cell

NASA Astrophysics Data System (ADS)

Burgmann, Sebastian; Blank, Mirja; Panchenko, Olha; Wartmann, Jens

2013-05-01

In direct methanol fuel cells (DMFCs), two-phase flows appear in the channels of the anode side (CO2 bubbles in a liquid water-methanol environment) as well as of the cathode side (water droplets or films in an ambient air flow). CO2 bubbles or water droplets may almost completely fill the cross-section of a channel. The instantaneous effect of the formation of two-phase flows on the cell performance has not been investigated in detail, yet. In the current project, the micro particle image velocimetry (μPIV) technique is used to elucidate the corresponding flow phenomena on the anode as well as on the cathode side of a DMFC and to correlate those phenomena with the performance of the cell. A single-channel DMFC with optical access at the anode and the cathode side is constructed and assembled that allows for μPIV measurements at both sides as well as a detailed time-resolved cell voltage recording. The appearance and evolution of CO2 bubbles on the anode side is qualitatively and quantitatively investigated. The results clearly indicate that the cell power increases when the free cross-section area of the channel is decreased by huge bubbles. Methanol is forced into the porous gas diffusion layer (GDL) between the channels and the membrane is oxidized to CO2, and hence, the fuel consumption is increased and the cell performance rises. Eventually, a bubble forms a moving slug that effectively cleans the channel from CO2 bubbles on its way downstream. The blockage effect is eliminated; the methanol flow is not forced into the GDL anymore. The remaining amount of methanol in the GDL is oxidized. The cell power decreases until enough CO2 is produced to eventually form bubbles again and the process starts again. On the other hand under the investigated conditions, water on the cathode side only forms liquid films on the channels walls rather than channel-filling droplets. Instantaneous changes of the cell power due to liquid water formation could not be observed. The timescales of the two-phase flow on the cathode side are significantly larger than on the anode side. However, the μPIV measurements at the cathode side demonstrate the ability of feeding gas flows in microchannels with liquid tracer particles and the ability to measure in two-phase flows in such a configuration.

A simple and highly sensitive spectroscopic fluorescence-detection system for multi-channel plastic-microchip electrophoresis based on side-entry laser-beam zigzag irradiation.

PubMed

Anazawa, Takashi; Uchiho, Yuichi; Yokoi, Takahide; Chalkidis, George; Yamazaki, Motohiro

2017-06-27

A five-color fluorescence-detection system for eight-channel plastic-microchip electrophoresis was developed. In the eight channels (with effective electrophoretic lengths of 10 cm), single-stranded DNA fragments were separated (with single-base resolution up to 300 bases within 10 min), and seventeen-loci STR genotyping for forensic human identification was successfully demonstrated. In the system, a side-entry laser beam is passed through the eight channels (eight A channels), with alternately arrayed seven sacrificial channels (seven B channels), by a technique called "side-entry laser-beam zigzag irradiation." Laser-induced fluorescence from the eight A channels and Raman-scattered light from the seven B channels are then simultaneously, uniformly, and spectroscopically detected, in the direction perpendicular to the channel array plane, through a transmission grating and a CCD camera. The system is therefore simple and highly sensitive. Because the microchip is fabricated by plastic-injection molding, it is inexpensive and disposable and thus suitable for actual use in various fields.

Application of ELJ to create and maintain side channels in a dynamic gravel bed river

NASA Astrophysics Data System (ADS)

Crabbe, E.; Crowe Curran, J.; Ockelford, A.

2017-12-01

Braided and anastomosing rivers create and maintain a large amount of side channel habitat. Unfortunately, many rivers that were once multi-channel rivers have been constrained to single thread channels as a consequence of land use changes that occurred in the 19th and 20th centuries or earlier. An increasingly common management goal today is the re-creation of self-maintaining side and tributary habitat through as natural means as possible. This work examines the geomorphic history of one such channel and the success of recent rehabilitation efforts. Our case study comes from the South Fork Nooksack River in the Cascades Range in Washington State. The Nooksack River is a gravel and sand bed channel with a snowmelt dominated hydrograph. Engineered log jams (ELJ) have been employed to direct flow into side and chute channels with the larger goals of increasing overall channel complexity and salmon spawning opportunities. ELJs have been constructed on the channel since the 2000s, and the ELJs in the study reaches range in age up to 10 years. The size and design of individual jams within the reach vary, enabling a comparison between jam types. ELJs are evaluated for their ability to maintain gravel bar locations and open tributary channels through the snowmelt season over the reach scale. Additional goals of trapping wood onto the jams and existing bars, stabilizing channel banks, and allowing for the growth of bar vegetation are also examined.

Extended analysis of the Trojan-horse attack in quantum key distribution

NASA Astrophysics Data System (ADS)

Vinay, Scott E.; Kok, Pieter

2018-04-01

The discrete-variable quantum key distribution protocols based on the 1984 protocol of Bennett and Brassard (BB84) are known to be secure against an eavesdropper, Eve, intercepting the flying qubits and performing any quantum operation on them. However, these protocols may still be vulnerable to side-channel attacks. We investigate the Trojan-horse side-channel attack where Eve sends her own state into Alice's apparatus and measures the reflected state to estimate the key. We prove that the separable coherent state is optimal for Eve among the class of multimode Gaussian attack states, even in the presence of thermal noise. We then provide a bound on the secret key rate in the case where Eve may use any separable state.

Coupling of the magnetic field and gas flows inferred from the net circular polarization in a sunspot penumbra

NASA Astrophysics Data System (ADS)

Shaltout, Abdelrazek M. K.; Ichimoto, Kiyoshi

2015-04-01

We analyze penumbral fine structure using high-resolution spectropolarimetric data obtained by the Solar Optical Telescope on board the Hinode satellite. The spatial correlation between the net circular polarization (NCP) and Evershed flow is investigated in detail. Here we obtain that negative NCP structures are correlated with the Evershed flow channels in the limb-side penumbra, and that negative NCP or depressions of positive NCP are associated with the Evershed flow channels in the disk center-side of the penumbra for a negative-polarity sunspot in NOAA 10923. The positive NCP dominant in the disk center-side penumbra is essentially attributed to interflow channels instead of Evershed flow channels. The stratification of magnetic field and velocity are investigated by using SIR-JUMP inversion with a one-component atmosphere, and the NCP of spectral lines in the limb-side and disk center-side of the penumbra is successfully reproduced. The inversion results show that an increased Evershed flow is associated with a strong magnetic field located in the deep photosphere. Our result does not match with the simple two-component penumbral models in which the penumbra consists of Evershed flow and interflow channels and the global NCP is attributed only to the Evershed flow channels.

Axially shaped channel and integral flow trippers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Crowther, R.L.; Johansson, E.B.; Matzner, B.

1988-06-07

A fuel assembly is described comprising fuel rods positioned in spaced array by upper and lower tie-plates, an open ended flow channel surrounding the array for conducting coolant upward between a lower support plate having coolant communicated thereto to an upper support grid having a steam/water outlet communicated thereto. The flow channel surrounds the array for conducting coolant about the fuel rods. The open ended channel has a polygon shaped cross section with the channel constituting a closed conduit with flat side sections connected at corners to form the enclosed conduit; means separate from the channel for connecting the uppermore » and lower tie-plates together and maintaining the fuel rods in spaced array independent of the flow channel. The improvement in the flow channel comprises tapered side walls. The tapered side walls extend from an average thick cross section adjacent the lower support plate to an average thin cross section adjacent the upper core grid whereby the channel is reduced in thickness adjacent the upper core grid to correspond with the reduced pressure adjacent the upper core grid.« less

Axially shaped channel and integral flow trippers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Crowther, R.L. Jr.; Johansson, E.B.; Matzner, B.

1992-02-11

This patent describes a fuel assembly. It comprises: fuel rods positioned in spaced array by upper and lower tie-plates, and open ended flow channel surrounding the array for conducting coolant upward between a lower support plate having coolant communicated thereto to an upper support grid having a steam/water outlet communicated thereto. The flow channel surrounding the array for conducting coolant about the fuel rods; the open ended channel having a polygon shaped cross section with the channel constituting a closed conduit with flat side sections connected at corners to form the enclosed conduit; means separate from the channel for connectingmore » the upper and lower tie-plates together and maintaining the fuel rods in spaced array independent of the flow channel, the improvement in the flow channel comprising tapered side walls, the tapered side walls extending from an average thick cross section adjacent the lower support plate to an average thin cross section adjacent the upper core grid whereby the channel is reduced in thickness adjacent the upper core grid to correspond with the reduced pressure adjacent the upper core grid.« less

Valve exploiting the principle of a side channel turbine

NASA Astrophysics Data System (ADS)

Jandourek, Pavel; Pochylý, František; Haban, Vladimír

2017-04-01

The presented article deals with a side channel turbine, which can be used as a suitable substitute for a pressure reducing valve. Pressure reducing valves are a source of high hydraulic losses. The aim is to replace them by a side channel turbine. With that in mind, hydraulic losses can be replaced by a production of electrical energy at comparable characteristics of the reducing valve and the side channel turbine. The basis for the design is the loss characteristics of the pressure reducing valve. Thereby create a new kind of turbine valve with speed-controlled flow in dependence of the runner revolution. It is technical innovation and new renewable source of energy, which can be in future used in rehabilitation or projecting of pumped-storage power plants. It also increases the power of the power plant.

Near wall cooling for a highly tapered turbine blade

DOEpatents

Liang, George [Palm City, FL

2011-03-08

A turbine blade having a pressure sidewall and a suction sidewall connected at chordally spaced leading and trailing edges to define a cooling cavity. Pressure and suction side inner walls extend radially within the cooling cavity and define pressure and suction side near wall chambers. A plurality of mid-chord channels extend radially from a radially intermediate location on the blade to a tip passage at the blade tip for connecting the pressure side and suction side near wall chambers in fluid communication with the tip passage. In addition, radially extending leading edge and trailing edge flow channels are located adjacent to the leading and trailing edges, respectively, and cooling fluid flows in a triple-pass serpentine path as it flows through the leading edge flow channel, the near wall chambers and the trailing edge flow channel.

Label-free viscosity measurement of complex fluids using reversal flow switching manipulation in a microfluidic channel

PubMed Central

Jun Kang, Yang; Ryu, Jeongeun; Lee, Sang-Joon

2013-01-01

The accurate viscosity measurement of complex fluids is essential for characterizing fluidic behaviors in blood vessels and in microfluidic channels of lab-on-a-chip devices. A microfluidic platform that accurately identifies biophysical properties of blood can be used as a promising tool for the early detections of cardiovascular and microcirculation diseases. In this study, a flow-switching phenomenon depending on hydrodynamic balancing in a microfluidic channel was adopted to conduct viscosity measurement of complex fluids with label-free operation. A microfluidic device for demonstrating this proposed method was designed to have two inlets for supplying the test and reference fluids, two side channels in parallel, and a junction channel connected to the midpoint of the two side channels. According to this proposed method, viscosities of various fluids with different phases (aqueous, oil, and blood) in relation to that of reference fluid were accurately determined by measuring the switching flow-rate ratio between the test and reference fluids, when a reverse flow of the test or reference fluid occurs in the junction channel. An analytical viscosity formula was derived to measure the viscosity of a test fluid in relation to that of the corresponding reference fluid using a discrete circuit model for the microfluidic device. The experimental analysis for evaluating the effects of various parameters on the performance of the proposed method revealed that the fluidic resistance ratio (RJL/RL, fluidic resistance in the junction channel (RJL) to fluidic resistance in the side channel (RL)) strongly affects the measurement accuracy. The microfluidic device with smaller RJL/RL values is helpful to measure accurately the viscosity of the test fluid. The proposed method accurately measured the viscosities of various fluids, including single-phase (Glycerin and plasma) and oil-water phase (oil vs. deionized water) fluids, compared with conventional methods. The proposed method was also successfully applied to measure viscosities of blood with varying hematocrits, chemically fixed RBCS, and channel sizes. Based on these experimental results, the proposed method can be effectively used to measure the viscosities of various fluids easily, without any fluorescent labeling and tedious calibration procedures. PMID:24404040

Component having cooling channel with hourglass cross section

DOEpatents

Campbell, Christian X; Lee, Ching-Pang

2015-04-28

A cooling channel (36, 36B, 63-66) cools inner surfaces (48, 50) of exterior walls (41, 43) of a component (20, 60). Interior side surfaces (52, 54) of the channel converge to a waist (W2), forming an hourglass shaped transverse profile (46). The inner surfaces (48, 50) may have fins (44) aligned with the coolant flow (22). The fins may have a transverse profile (56A, 56B) highest at mid-width of the inner surfaces (48, 50). Turbulators (92) may be provided on the side surfaces (52, 54) of the channel, and may urge the coolant flow toward the inner surfaces (48, 50). Each turbulator (92) may have a peak (97) that defines the waist of the cooling channel. Each turbulator may have a convex upstream side (93). These elements increase coolant flow in the corners (C) of the channel to more uniformly and efficiently cool the exterior walls (41, 43).

Cardiac ion channels

PubMed Central

Priest, Birgit T; McDermott, Jeff S

2015-01-01

Ion channels are critical for all aspects of cardiac function, including rhythmicity and contractility. Consequently, ion channels are key targets for therapeutics aimed at cardiac pathophysiologies such as atrial fibrillation or angina. At the same time, off-target interactions of drugs with cardiac ion channels can be the cause of unwanted side effects. This manuscript aims to review the physiology and pharmacology of key cardiac ion channels. The intent is to highlight recent developments for therapeutic development, as well as elucidate potential mechanisms for drug-induced cardiac side effects, rather than present an in-depth review of each channel subtype. PMID:26556552

Electrical characteristics of tunneling field-effect transistors with asymmetric channel thickness

NASA Astrophysics Data System (ADS)

Kim, Jungsik; Oh, Hyeongwan; Kim, Jiwon; Meyyappan, M.; Lee, Jeong-Soo

2017-02-01

Effects of using asymmetric channel thickness in tunneling field-effect transistors (TFET) are investigated in sub-50 nm channel regime using two-dimensional (2D) simulations. As the thickness of the source side becomes narrower in narrow-source wide-drain (NSWD) TFETs, the threshold voltage (V th) and the subthreshold swing (SS) decrease due to enhanced gate controllability of the source side. The narrow source thickness can make the band-to-band tunneling (BTBT) distance shorter and induce much higher electric field near the source junction at the on-state condition. In contrast, in a TFET with wide-source narrow-drain (WSND), the SS shows almost constant values and the V th slightly increases with narrowing thickness of the drain side. In addition, the ambipolar current can rapidly become larger with smaller thickness on the drain side because of the shorter BTBT distance and the higher electric-field at the drain junction. The on-current of the asymmetric channel TFET is lower than that of conventional TFETs due to the volume limitation of the NSWD TFET and high series resistance of the WSND TFET. The on-current is almost determined by the channel thickness of the source side.

Side Fenestrations Provide an "Anchor" for a Stable Binding of A1899 to the Pore of TASK-1 Potassium Channels.

PubMed

Ramírez, David; Arévalo, Bárbara; Martínez, Gonzalo; Rinné, Susanne; Sepúlveda, Francisco V; Decher, Niels; González, Wendy

2017-07-03

A1899 is a potent and selective inhibitor of the two-pore domain potassium (K 2P ) channel TASK-1. It was previously reported that A1899 acts as an open-channel blocker and binds to residues of the P1 and P2 regions, the M2 and M4 segments, and the halothane response element. The recently described crystal structures of K 2P channels together with the newly identified side fenestrations indicate that residues relevant for TASK-1 inhibition are not purely facing the central cavity as initially proposed. Accordingly, the TASK-1 binding site and the mechanism of inhibition might need a re-evaluation. We have used TASK-1 homology models based on recently crystallized K 2P channels and molecular dynamics simulation to demonstrate that the highly potent TASK-1 blocker A1899 requires binding to residues located in the side fenestrations. Unexpectedly, most of the previously described residues that interfere with TASK-1 blockade by A1899 project their side chains toward the fenestration lumina, underlining the relevance of these structures for drug binding in K 2P channels. Despite its hydrophobicity, A1899 does not seem to use the fenestrations to gain access to the central cavity from the lipid bilayer. In contrast, binding of A1899 to residues of the side fenestrations might provide a physical "anchor", reflecting an energetically favorable binding mode that after pore occlusion stabilizes the closed state of the channels.

Visual communications with side information via distributed printing channels: extended multimedia and security perspectives

NASA Astrophysics Data System (ADS)

Voloshynovskiy, Sviatoslav V.; Koval, Oleksiy; Deguillaume, Frederic; Pun, Thierry

2004-06-01

In this paper we address visual communications via printing channels from an information-theoretic point of view as communications with side information. The solution to this problem addresses important aspects of multimedia data processing, security and management, since printed documents are still the most common form of visual information representation. Two practical approaches to side information communications for printed documents are analyzed in the paper. The first approach represents a layered joint source-channel coding for printed documents. This approach is based on a self-embedding concept where information is first encoded assuming a Wyner-Ziv set-up and then embedded into the original data using a Gel'fand-Pinsker construction and taking into account properties of printing channels. The second approach is based on Wyner-Ziv and Berger-Flynn-Gray set-ups and assumes two separated communications channels where an appropriate distributed coding should be elaborated. The first printing channel is considered to be a direct visual channel for images ("analog" channel with degradations). The second "digital channel" with constrained capacity is considered to be an appropriate auxiliary channel. We demonstrate both theoretically and practically how one can benefit from this sort of "distributed paper communications".

Valve exploiting the principle of a side channel turbine

NASA Astrophysics Data System (ADS)

Jandourek, Pavel; Habán, Vladimír; Pochylý, František; Fic, Miloslav

The article deals with a side channel turbine, which can be used as a suitable substitute for a pressure reducing valve. Reducing valves are a source of hydraulic losses. The aim is to replace them by a side channel turbine. With that in mind, hydraulic losses can be replaced by a production of electrical energy at comparable characteristics of the valve and the turbine. The basis for the design is the loss characteristics of the valve. Thereby creating a kind of turbine valve with speed-controlled flow in dependence of runner revolution.

Integrated Hydrogeological Investigation on the Vulnerability of a Pumping Station at a Losing Stream

NASA Astrophysics Data System (ADS)

Ngueleu Kamangou, Stephane; Vogt, Tobias; Cirpka, Olaf

2010-05-01

River restoration usually includes alteration of the river channel morphology. Thereby the interaction between river and groundwater can be modified. For the design of a river restoration project - especially in the vicinity of a groundwater pumping well for drinking water production - this impact must be predicted. But a good prediction requires a proper understanding of the existing situation. Numerical models help to improve the strategy of a successful river restoration project. The main objective of this study was to investigate the vulnerability of a pumping station located at losing river in northeast Switzerland. Besides the effect that river restoration could create, a particular attention was placed on the effect of a beaver dam in a side channel close to the pumping station. Analysis of field measurements coupled with numerical modeling of the pumping station area improved the understanding of the interactions in the river corridor between the river, side channels and the alluvial aquifer.

Electrofishing effort requirements for estimating species richness in the Kootenai River, Idaho

USGS Publications Warehouse

Watkins, Carson J.; Quist, Michael C.; Shepard, Bradley B.; Ireland, Susan C.

2016-01-01

This study was conducted on the Kootenai River, Idaho to provide insight on sampling requirements to optimize future monitoring effort associated with the response of fish assemblages to habitat rehabilitation. Our objective was to define the electrofishing effort (m) needed to have a 95% probability of sampling 50, 75, and 100% of the observed species richness and to evaluate the relative influence of depth, velocity, and instream woody cover on sample size requirements. Sidechannel habitats required more sampling effort to achieve 75 and 100% of the total species richness than main-channel habitats. The sampling effort required to have a 95% probability of sampling 100% of the species richness was 1100 m for main-channel sites and 1400 m for side-channel sites. We hypothesized that the difference in sampling requirements between main- and side-channel habitats was largely due to differences in habitat characteristics and species richness between main- and side-channel habitats. In general, main-channel habitats had lower species richness than side-channel habitats. Habitat characteristics (i.e., depth, current velocity, and woody instream cover) were not related to sample size requirements. Our guidelines will improve sampling efficiency during monitoring effort in the Kootenai River and provide insight on sampling designs for other large western river systems where electrofishing is used to assess fish assemblages.

Implementation of controlled quantum teleportation with an arbitrator for secure quantum channels via quantum dots inside optical cavities.

PubMed

Heo, Jino; Hong, Chang-Ho; Kang, Min-Sung; Yang, Hyeon; Yang, Hyung-Jin; Hong, Jong-Phil; Choi, Seong-Gon

2017-11-02

We propose a controlled quantum teleportation scheme to teleport an unknown state based on the interactions between flying photons and quantum dots (QDs) confined within single- and double-sided cavities. In our scheme, users (Alice and Bob) can teleport the unknown state through a secure entanglement channel under the control and distribution of an arbitrator (Trent). For construction of the entanglement channel, Trent utilizes the interactions between two photons and the QD-cavity system, which consists of a charged QD (negatively charged exciton) inside a single-sided cavity. Subsequently, Alice can teleport the unknown state of the electron spin in a QD inside a double-sided cavity to Bob's electron spin in a QD inside a single-sided cavity assisted by the channel information from Trent. Furthermore, our scheme using QD-cavity systems is feasible with high fidelity, and can be experimentally realized with current technologies.

2D modeling based comprehensive analysis of short channel effects in DMG strained VSTB FET

NASA Astrophysics Data System (ADS)

Saha, Priyanka; Banerjee, Pritha; Sarkar, Subir Kumar

2018-06-01

The paper aims to develop two dimensional analytical model of the proposed dual material (DM) Vertical Super Thin Body (VSTB) strained Field Effect Transistor (FET) with focus on its short channel behaviour in nanometer regime. Electrostatic potential across gate/channel and dielectric wall/channel interface is derived by solving 2D Poisson's equation with parabolic approximation method by applying appropriate boundary conditions. Threshold voltage is then calculated by using the criteria of minimum surface potential considering both gate and dielectric wall side potential. Performance analysis of the present structure is demonstrated in terms of potential, electric field, threshold voltage characteristics and subthreshold behaviour by varying various device parameters and applied biases. Effect of application of strain in channel is further explored to establish the superiority of the proposed device in comparison to conventional VSTB FET counterpart. All analytical results are compared with Silvaco ATLAS device simulated data to substantiate the accuracy of our derived model.

Detector-device-independent quantum key distribution: Security analysis and fast implementation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boaron, Alberto; Korzh, Boris; Houlmann, Raphael

One of the most pressing issues in quantum key distribution (QKD) is the problem of detector side-channel attacks. To overcome this problem, researchers proposed an elegant “time-reversal” QKD protocol called measurement-device-independent QKD (MDI-QKD), which is based on time-reversed entanglement swapping. But, MDI-QKD is more challenging to implement than standard point-to-point QKD. Recently, we proposed an intermediary QKD protocol called detector-device-independent QKD (DDI-QKD) in order to overcome the drawbacks of MDI-QKD, with the hope that it would eventually lead to a more efficient detector side-channel-free QKD system. We analyze the security of DDI-QKD and elucidate its security assumptions. We find thatmore » DDI-QKD is not equivalent to MDI-QKD, but its security can be demonstrated with reasonable assumptions. On the more practical side, we consider the feasibility of DDI-QKD and present a fast experimental demonstration (clocked at 625 MHz), capable of secret key exchange up to more than 90 km.« less

Detector-device-independent quantum key distribution: Security analysis and fast implementation

DOE PAGES

Boaron, Alberto; Korzh, Boris; Houlmann, Raphael; ...

2016-08-09

One of the most pressing issues in quantum key distribution (QKD) is the problem of detector side-channel attacks. To overcome this problem, researchers proposed an elegant “time-reversal” QKD protocol called measurement-device-independent QKD (MDI-QKD), which is based on time-reversed entanglement swapping. But, MDI-QKD is more challenging to implement than standard point-to-point QKD. Recently, we proposed an intermediary QKD protocol called detector-device-independent QKD (DDI-QKD) in order to overcome the drawbacks of MDI-QKD, with the hope that it would eventually lead to a more efficient detector side-channel-free QKD system. We analyze the security of DDI-QKD and elucidate its security assumptions. We find thatmore » DDI-QKD is not equivalent to MDI-QKD, but its security can be demonstrated with reasonable assumptions. On the more practical side, we consider the feasibility of DDI-QKD and present a fast experimental demonstration (clocked at 625 MHz), capable of secret key exchange up to more than 90 km.« less

The conserved potassium channel filter can have distinct ion binding profiles: Structural analysis of rubidium, cesium, and barium binding in NaK2K

PubMed Central

Lam, Yee Ling; Zeng, Weizhong; Sauer, David Bryant

2014-01-01

Potassium channels are highly selective for K+ over the smaller Na+. Intriguingly, they are permeable to larger monovalent cations such as Rb+ and Cs+ but are specifically blocked by the similarly sized Ba2+. In this study, we used structural analysis to determine the binding profiles for these permeant and blocking ions in the selectivity filter of the potassium-selective NaK channel mutant NaK2K and also performed permeation experiments using single-channel recordings. Our data revealed that some ion binding properties of NaK2K are distinct from those of the canonical K+ channels KcsA and MthK. Rb+ bound at sites 1, 3, and 4 in NaK2K, as it does in KcsA. Cs+, however, bound predominantly at sites 1 and 3 in NaK2K, whereas it binds at sites 1, 3, and 4 in KcsA. Moreover, Ba2+ binding in NaK2K was distinct from that which has been observed in KcsA and MthK, even though all of these channels show similar Ba2+ block. In the presence of K+, Ba2+ bound to the NaK2K channel at site 3 in conjunction with a K+ at site 1; this led to a prolonged block of the channel (the external K+-dependent Ba2+ lock-in state). In the absence of K+, however, Ba2+ acts as a permeating blocker. We found that, under these conditions, Ba2+ bound at sites 1 or 0 as well as site 3, allowing it to enter the filter from the intracellular side and exit from the extracellular side. The difference in the Ba2+ binding profile in the presence and absence of K+ thus provides a structural explanation for the short and prolonged Ba2+ block observed in NaK2K. PMID:25024267

The conserved potassium channel filter can have distinct ion binding profiles: structural analysis of rubidium, cesium, and barium binding in NaK2K.

PubMed

Lam, Yee Ling; Zeng, Weizhong; Sauer, David Bryant; Jiang, Youxing

2014-08-01

Potassium channels are highly selective for K(+) over the smaller Na(+). Intriguingly, they are permeable to larger monovalent cations such as Rb(+) and Cs(+) but are specifically blocked by the similarly sized Ba(2+). In this study, we used structural analysis to determine the binding profiles for these permeant and blocking ions in the selectivity filter of the potassium-selective NaK channel mutant NaK2K and also performed permeation experiments using single-channel recordings. Our data revealed that some ion binding properties of NaK2K are distinct from those of the canonical K(+) channels KcsA and MthK. Rb(+) bound at sites 1, 3, and 4 in NaK2K, as it does in KcsA. Cs(+), however, bound predominantly at sites 1 and 3 in NaK2K, whereas it binds at sites 1, 3, and 4 in KcsA. Moreover, Ba(2+) binding in NaK2K was distinct from that which has been observed in KcsA and MthK, even though all of these channels show similar Ba(2+) block. In the presence of K(+), Ba(2+) bound to the NaK2K channel at site 3 in conjunction with a K(+) at site 1; this led to a prolonged block of the channel (the external K(+)-dependent Ba(2+) lock-in state). In the absence of K(+), however, Ba(2+) acts as a permeating blocker. We found that, under these conditions, Ba(2+) bound at sites 1 or 0 as well as site 3, allowing it to enter the filter from the intracellular side and exit from the extracellular side. The difference in the Ba(2+) binding profile in the presence and absence of K(+) thus provides a structural explanation for the short and prolonged Ba(2+) block observed in NaK2K. © 2014 Lam et al.

Gating of a pH-sensitive K(2P) potassium channel by an electrostatic effect of basic sensor residues on the selectivity filter.

PubMed

Zúñiga, Leandro; Márquez, Valeria; González-Nilo, Fernando D; Chipot, Christophe; Cid, L Pablo; Sepúlveda, Francisco V; Niemeyer, María Isabel

2011-01-25

K(+) channels share common selectivity characteristics but exhibit a wide diversity in how they are gated open. Leak K(2P) K(+) channels TASK-2, TALK-1 and TALK-2 are gated open by extracellular alkalinization. The mechanism for this alkalinization-dependent gating has been proposed to be the neutralization of the side chain of a single arginine (lysine in TALK-2) residue near the pore of TASK-2, which occurs with the unusual pK(a) of 8.0. We now corroborate this hypothesis by transplanting the TASK-2 extracellular pH (pH(o)) sensor in the background of a pH(o)-insensitive TASK-3 channel, which leads to the restitution of pH(o)-gating. Using a concatenated channel approach, we also demonstrate that for TASK-2 to open, pH(o) sensors must be neutralized in each of the two subunits forming these dimeric channels with no apparent cross-talk between the sensors. These results are consistent with adaptive biasing force analysis of K(+) permeation using a model selectivity filter in wild-type and mutated channels. The underlying free-energy profiles confirm that either a doubly or a singly charged pH(o) sensor is sufficient to abolish ion flow. Atomic detail of the associated mechanism reveals that, rather than a collapse of the pore, as proposed for other K(2P) channels gated at the selectivity filter, an increased height of the energetic barriers for ion translocation accounts for channel blockade at acid pH(o). Our data, therefore, strongly suggest that a cycle of protonation/deprotonation of pH(o)-sensing arginine 224 side chain gates the TASK-2 channel by electrostatically tuning the conformational stability of its selectivity filter.

Gating of a pH-Sensitive K2P Potassium Channel by an Electrostatic Effect of Basic Sensor Residues on the Selectivity Filter

PubMed Central

Zúñiga, Leandro; Márquez, Valeria; González-Nilo, Fernando D.; Chipot, Christophe; Cid, L. Pablo; Sepúlveda, Francisco V.; Niemeyer, María Isabel

2011-01-01

K+ channels share common selectivity characteristics but exhibit a wide diversity in how they are gated open. Leak K2P K+ channels TASK-2, TALK-1 and TALK-2 are gated open by extracellular alkalinization. The mechanism for this alkalinization-dependent gating has been proposed to be the neutralization of the side chain of a single arginine (lysine in TALK-2) residue near the pore of TASK-2, which occurs with the unusual pKa of 8.0. We now corroborate this hypothesis by transplanting the TASK-2 extracellular pH (pHo) sensor in the background of a pHo-insensitive TASK-3 channel, which leads to the restitution of pHo-gating. Using a concatenated channel approach, we also demonstrate that for TASK-2 to open, pHo sensors must be neutralized in each of the two subunits forming these dimeric channels with no apparent cross-talk between the sensors. These results are consistent with adaptive biasing force analysis of K+ permeation using a model selectivity filter in wild-type and mutated channels. The underlying free-energy profiles confirm that either a doubly or a singly charged pHo sensor is sufficient to abolish ion flow. Atomic detail of the associated mechanism reveals that, rather than a collapse of the pore, as proposed for other K2P channels gated at the selectivity filter, an increased height of the energetic barriers for ion translocation accounts for channel blockade at acid pHo. Our data, therefore, strongly suggest that a cycle of protonation/deprotonation of pHo-sensing arginine 224 side chain gates the TASK-2 channel by electrostatically tuning the conformational stability of its selectivity filter. PMID:21283586

Wavy flow cooling concept for turbine airfoils

DOEpatents

Liang, George

2010-08-31

An airfoil including an outer wall and a cooling cavity formed therein. The cooling cavity includes a leading edge flow channel located adjacent a leading edge of the airfoil and a trailing edge flow channel located adjacent a trailing edge of the airfoil. Each of the leading edge and trailing edge flow channels define respective first and second flow axes located between pressure and suction sides of the airfoil. A plurality of rib members are located within each of the flow channels, spaced along the flow axes, and alternately extending from opposing sides of the flow channels to define undulating flow paths through the flow channels.

The role of cystic fibrosis transmembrane conductance regulator phenylalanine 508 side chain in ion channel gating

PubMed Central

Cui, Liying; Aleksandrov, Luba; Hou, Yue-Xian; Gentzsch, Martina; Chen, Jey-Hsin; Riordan, John R; Aleksandrov, Andrei A

2006-01-01

Cystic fibrosis transmembrane conductance regulator (CFTR) is an ion channel employing the ABC transporter structural motif. Deletion of a single residue (Phe508) in the first nucleotide-binding domain (NBD1), which occurs in most patients with cystic fibrosis, impairs both maturation and function of the protein. However, substitution of the Phe508 with small uncharged amino acids, including cysteine, is permissive for maturation. To explore the possible role of the phenylalanine aromatic side chain in channel gating we introduced a cysteine at this position in cysless CFTR, enabling its selective chemical modification by sulfhydryl reagents. Both cysless and wild-type CFTR ion channels have identical mean open times when activated by different nucleotide ligands. Moreover, both channels could be locked in an open state by introducing an ATPase inhibiting mutation (E1371S). However, the introduction of a single cysteine (F508C) prevented the cysless E1371S channel from maintaining the permanently open state, allowing closing to occur. Chemical modification of cysless E1371S/F508C by sulfhydryl reagents was used to probe the role of the side chain in ion channel function. Specifically, benzyl-methanethiosulphonate modification of this variant restored the gating behaviour to that of cysless E1371S containing the wild-type phenylalanine at position 508. This provides the first direct evidence that a specific interaction of the Phe508 aromatic side chain plays a role in determining the residency time in the closed state. Thus, despite the fact that this aromatic side chain is not essential for CFTR folding, it is important in the ion channel function. PMID:16484308

GREAT II (Upper Mississippi River. Guttenberg, Iowa to Saverton, Missouri). Side Channel Work Group Appendix

DTIC Science & Technology

1980-12-01

is current during normal river stage. The graduations in this category are wide- spread, ranging frcm fast flowing watercourses with high banks to...channel category on the other. They may be former side channels that have been cut off, or that have only intermittent flows in them. They may be...navigation project certainly is a contributor by providing a deep channel and an abundance of beaches. Karaki and Van Hoften noted that small, fast

Effect of graded InGaN drain region and 'In' fraction in InGaN channel on performances of InGaN tunnel field-effect transistor

NASA Astrophysics Data System (ADS)

Duan, Xiaoling; Zhang, Jincheng; Wang, Shulong; Quan, Rudai; Hao, Yue

2017-12-01

An InGaN-based graded drain region tunnel field-effect transistor (GD-TFET) is proposed to suppress the ambipolar behavior. The simulation results with the trade-off between on-state current (Ion) and ambipolar current (Iambipolar) show decreased Iambipolar (1.9 × 10-14 A/μm) in comparison with that of conventional TFETs (2.0 × 10-8 A/μm). Furthermore, GD-TFET with high 'In' fraction InxGa1-xN source-side channel (SC- GD-TFET) is explored and exhibits 5.3 times Ion improvement and 60% average subthreshold swing (SSavg) reduction in comparison with GD-TFET by adjusting 'In' fraction in the InxGa1-xN source-side channel. The improvement is attributed to the confinement of BTBT in the source-side channel by the heterojunction. And then, the optimum value for source-side channel length (Lsc) is researched by DC performances results, which shows it falls into the range between Lsc = 10 nm and 20 nm.

Enhancing Electromagnetic Side-Channel Analysis in an Operational Environment

DTIC Science & Technology

2013-09-01

phenomenon of compromising power and EM emissions has been known and exploited for decades. Declassified TEMPEST documents reveal vulnerabilities of...Components. One technique to detect potentially compromising emissions is to use a wide-band receiver tuned to a specific frequency. High-end TEMPEST

Continuous cell introduction and rapid dynamic lysis for high-throughput single-cell analysis on microfludic chips with hydrodynamic focusing.

PubMed

Xu, Chun-Xiu; Yin, Xue-Feng

2011-02-04

A chip-based microfluidic system for high-throughput single-cell analysis is described. The system was integrated with continuous introduction of individual cells, rapid dynamic lysis, capillary electrophoretic (CE) separation and laser induced fluorescence (LIF) detection. A cross microfluidic chip with one sheath-flow channel located on each side of the sampling channel was designed. The labeled cells were hydrodynamically focused by sheath-flow streams and sequentially introduced into the cross section of the microchip under hydrostatic pressure generated by adjusting liquid levels in the reservoirs. Combined with the electric field applied on the separation channel, the aligned cells were driven into the separation channel and rapidly lysed within 33ms at the entry of the separation channel by Triton X-100 added in the sheath-flow solution. The maximum rate for introducing individual cells into the separation channel was about 150cells/min. The introduction of sheath-flow streams also significantly reduced the concentration of phosphate-buffered saline (PBS) injected into the separation channel along with single cells, thus reducing Joule heating during electrophoretic separation. The performance of this microfluidic system was evaluated by analysis of reduced glutathione (GSH) and reactive oxygen species (ROS) in single erythrocytes. A throughput of 38cells/min was obtained. The proposed method is simple and robust for high-throughput single-cell analysis, allowing for analysis of cell population with considerable size to generate results with statistical significance. Copyright © 2010 Elsevier B.V. All rights reserved.

Tunable all-optical photonic crystal channel drop filter for DWDM systems

NASA Astrophysics Data System (ADS)

Habibiyan, H.; Ghafoori-Fard, H.; Rostami, A.

2009-06-01

In this paper we propose a tunable channel drop filter in a two-dimensional photonic crystal, based on coupled-cavity waveguides with alternating small and large defects and an electromagnetically induced transparency phenomenon. By utilizing this phenomenon a narrower linewidth is obtained and also the frequency of the dropped signal becomes tunable. Simulation results show that the proposed filter is suitable for dense wavelength-division multiplexing (DWDM) systems with 0.8 nm channel spacing. Using this novel component, two ultrasmall eight-channel double-sided and single-sided demultiplexers are introduced. The properties of these devices are investigated using the finite-difference time-domain method. For the single-sided device, transmission loss is 1.5 ± 0.5 dB, the cross-talk level between adjacent channels is better than -18 dB and the average 3 dB optical passband is 0.36 nm. Using planar silicon-on-insulator technology, the physical area for the single-sided component is 700 µm2 and for the double-sided component is 575 µm2. To the best of our knowledge, these are the smallest all-optical demultiplexers with this spectral resolution reported to date. Malfunction of the proposed device due to fabrication errors is modeled and its tunable characteristic is demonstrated.

Low resistance, large dimension entrance to the inner cavity of BK channels determined by changing side-chain volume

PubMed Central

Niu, Xiaowei

2011-01-01

Large-conductance Ca2+- and voltage-activated K+ (BK) channels have the largest conductance (250–300 pS) of all K+-selective channels. Yet, the contributions of the various parts of the ion conduction pathway to the conductance are not known. Here, we examine the contribution of the entrance to the inner cavity to the large conductance. Residues at E321/E324 on each of the four α subunits encircle the entrance to the inner cavity. To determine if 321/324 is accessible from the inner conduction pathway, we measured single-channel current amplitudes before and after exposure and wash of thiol reagents to the intracellular side of E321C and E324C channels. MPA− increased currents and MTSET+ decreased currents, with no difference between positions 321 and 324, indicating that side chains at 321/324 are accessible from the inner conduction pathway and have equivalent effects on conductance. For neutral amino acids, decreasing the size of the entrance to the inner cavity by substituting large side-chain amino acids at 321/324 decreased outward single-channel conductance, whereas increasing the size of the entrance with smaller side-chain substitutions had little effect. Reductions in outward conductance were negated by high [K+]i. Substitutions had little effect on inward conductance. Fitting plots of conductance versus side-chain volume with a model consisting of one variable and one fixed resistor in series indicated an effective diameter and length of the entrance to the inner cavity for wild-type channels of 17.7 and 5.6 Å, respectively, with the resistance of the entrance ∼7% of the total resistance of the conduction pathway. The estimated dimensions are consistent with the structure of MthK, an archaeal homologue to BK channels. Our observations suggest that BK channels have a low resistance, large entrance to the inner cavity, with the entrance being as large as necessary to not limit current, but not much larger. PMID:21576375

Mixing in the shear superposition micromixer: three-dimensional analysis.

PubMed

Bottausci, Frederic; Mezić, Igor; Meinhart, Carl D; Cardonne, Caroline

2004-05-15

In this paper, we analyse mixing in an active chaotic advection micromixer. The micromixer consists of a main rectangular channel and three cross-stream secondary channels that provide ability for time-dependent actuation of the flow stream in the direction orthogonal to the main stream. Three-dimensional motion in the mixer is studied. Numerical simulations and modelling of the flow are pursued in order to understand the experiments. It is shown that for some values of parameters a simple model can be derived that clearly represents the flow nature. Particle image velocimetry measurements of the flow are compared with numerical simulations and the analytical model. A measure for mixing, the mixing variance coefficient (MVC), is analysed. It is shown that mixing is substantially improved with multiple side channels with oscillatory flows, whose frequencies are increasing downstream. The optimization of MVC results for single side-channel mixing is presented. It is shown that dependence of MVC on frequency is not monotone, and a local minimum is found. Residence time distributions derived from the analytical model are analysed. It is shown that, while the average Lagrangian velocity profile is flattened over the steady flow, Taylor-dispersion effects are still present for the current micromixer configuration.

Novel four-sided neural probe fabricated by a thermal lamination process of polymer films.

PubMed

Shin, Soowon; Kim, Jae-Hyun; Jeong, Joonsoo; Gwon, Tae Mok; Lee, Seung-Hee; Kim, Sung June

2017-02-15

Ideally, neural probes should have channels with a three-dimensional (3-D) configuration to record the activities of 3-D neural circuits. Many types of 3-D neural probes have been developed; however, most of them were designed as an array of multiple shanks with electrodes located along one side of the shanks. We developed a novel liquid crystal polymer (LCP)-based neural probe with four-sided electrodes. This probe has electrodes on four sides of the shank, i.e., the front, back and two sidewalls. To generate the proposed configuration of the electrodes, we used a thermal lamination process involving LCP films and laser micromachining. The proposed novel four-sided neural probe, was used to successfully perform in vivo multichannel neural recording in the mouse primary somatosensory cortex. The multichannel neural recording showed that the proposed four-sided neural probe can record spiking activities from a more diverse neuronal population than single-sided probes. This was confirmed by a pairwise Pearson correlation coefficient (Pearson's r) analysis and a cross-correlation analysis. The developed four-sided neural probe can be used to record various signals from a complex neural network. Copyright © 2016 Elsevier B.V. All rights reserved.

Electrically controlled wire-channel GaN/AlGaN transistor for terahertz plasma applications

NASA Astrophysics Data System (ADS)

Cywiński, G.; Yahniuk, I.; Kruszewski, P.; Grabowski, M.; Nowakowski-Szkudlarek, K.; Prystawko, P.; Sai, P.; Knap, W.; Simin, G. S.; Rumyantsev, S. L.

2018-03-01

We report on a design of fin-shaped channel GaN/AlGaN field-effect transistors developed for studying resonant terahertz plasma oscillations. Unlike common two dimensional FinFET transistor design, the gates were deposited only to the sides of the two dimensional electron gas channel, i.e., metal layers were not deposited on the top of the AlGaN. This side gate configuration allowed us to electrically control the conductivity of the channel by changing its width while keeping the carrier density and mobility virtually unchanged. Computer simulations and analytical model describe well the general shape of the characteristics. The side gate control of the channel width of these transistors allowed us to eliminate the so-called oblique plasma wave modes and paves the way towards future terahertz detectors and emitters using high quality factor plasma wave resonances.

The floodplain food web mosaic: a study of its importance to salmon and steelhead with implications for their recovery

USGS Publications Warehouse

Bellmore, J. Ryan; Baxter, Colden V.; Martens, Kyle; Connolly, Patrick J.

2013-01-01

Although numerous studies have attempted to place species of interest within the context of food webs, such efforts have generally occurred at small scales or disregard potentially important spatial heterogeneity. If food web approaches are to be employed to manage species, studies are needed that evaluate the multiple habitats and associated webs of interactions in which these species participate. Here, we quantify the food webs that sustain rearing salmon and steelhead within a floodplain landscape of the Methow River, Washington, USA, a location where restoration has been proposed to restore side channels in an attempt to recover anadromous fishes. We combined year-long measures of production, food demand, and diet composition for the fish assemblage with estimates of invertebrate prey productivity to quantify food webs within the main channel and five different, intact, side channels; ranging from channels that remained connected to the main channel at low flow to those reduced to floodplain ponds. Although we found that habitats within the floodplain had similar invertebrate prey production, these habitats hosted different local food webs. In the main channel, 95% of total prey consumption flowed to fishes that are not the target of proposed restoration. These fishes consumed 64% and 47% of the prey resources that were found to be important to fueling chinook and steelhead production in the main channel, respectively. Conversely, in side channels, a greater proportion of prey was consumed by anadromous salmonids. As a result, carrying capacity estimates based on food were 251% higher, on average, for anadromous salmonids in side channels than the main channel. However, salmon and steelhead production was generally well below estimated capacity in both the main and side channels, suggesting these habitats are under-seeded with respect to food, and that much larger populations could be supported. Overall, this study demonstrates that floodplain heterogeneity is associated with the occurrence of a mosaic of food webs, all of which were utilized by anadromous salmonids, and all of which may be important to their recovery and persistence. In the long term, these and other fishes would likely benefit from restoring the processes that maintain floodplain complexity.

Identification of a Novel EF-Loop in the N-terminus of TRPM2 Channel Involved in Calcium Sensitivity

PubMed Central

Luo, Yuhuan; Yu, Xiafei; Ma, Cheng; Luo, Jianhong; Yang, Wei

2018-01-01

As an oxidative stress sensor, transient receptor potential melastatin 2 (TRPM2) channel is involved in many physiological and pathological processes including warmth sensing, ischemia injury, inflammatory diseases and diabetes. Intracellular calcium is critical for TRPM2 channel activation and the IQ-like motif in the N-terminus has been shown to be important by mediating calmodulin binding. Sequence analysis predicted two potential EF-loops in the N-terminus of TRPM2. Site-directed mutagenesis combining with functional assay showed that substitution with alanine of several residues, most of which are conserved in the typical EF-loop, including D267, D278, D288, and E298 dramatically reduced TRPM2 channel currents. By further changing the charges or side chain length of these conserved residues, our results indicate that the negative charge of D267 and the side chain length of D278 are critical for calcium-induced TRPM2 channel activation. G272I mutation also dramatically reduced the channel currents, suggesting that this site is critical for calcium-induced TRPM2 channel activation. Furthermore, D267A mutant dramatically reduced the currents induced by calcium alone compared with that by ADPR, indicating that D267 residue in D267–D278 motif is the most important site for calcium sensitivity of TRPM2. In addition, inside-out recordings showed that mutations at D267, G272, D278, and E298 had no effect on single-channel conductance. Taken together, our data indicate that D267–D278 motif in the N-terminus as a novel EF-loop is critical for calcium-induced TRPM2 channel activation.

Optofluidic devices and methods for sensing single particles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fernandez-Cuesta, Irene; Cabrini, Stefano

This disclosure provides systems, methods, and apparatus related to optofluidic devices. In one aspect, an optofluidic device includes a substrate, a first nanostructure, a second nanostructure, and a cover. A channel having cross-sectional dimensions of less than about 100 nanometers is defined in a surface of the substrate. The first nanostructure is disposed on the substrate on a first side of the channel and proximate the channel. The second nanostructure is disposed on the substrate on a second side of the channel and proximate the channel. The first and the second nanostructures are disposed on a line that passes acrossmore » the channel. The cover is disposed on the surface of the substrate.« less

Prevention of Information Leakage by Photo-Coupling in Smart Card

NASA Astrophysics Data System (ADS)

Shen, Sung-Shiou; Chiu, Jung-Hui

Advances in smart card technology encourages smart card use in more sensitive applications, such as storing important information and securing application. Smart cards are however vulnerable to side channel attacks. Power consumption and electromagnetic radiation of the smart card can leak information about the secret data protected by the smart card. Our paper describes two possible hardware countermeasures that protect against side channel information leakage. We show that power analysis can be prevented by adopting photo-coupling techniques. This method involves the use of LED with photovoltaic cells and photo-couplers on the power, reset, I/O and clock lines of the smart card. This method reduces the risk of internal data bus leakage on the external data lines. Moreover, we also discuss the effectiveness of reducing electromagnetic radiation by using embedded metal plates.

Fabrication and Characterization of All-Polystyrene Microfluidic Devices with Integrated Electrodes and Tubing.

PubMed

Pentecost, Amber M; Martin, R Scott

2015-01-01

A new method of fabricating all-polystyrene devices with integrated electrodes and fluidic tubing is described. As opposed to expensive polystyrene (PS) fabrication techniques that use hot embossing and bonding with a heated lab press, this approach involves solvent-based etching of channels and lamination-based bonding of a PS cover, all of which do not need to occur in a clean room. PS has been studied as an alternative microchip substrate to PDMS, as it is more hydrophilic, biologically compatible in terms of cell adhesion, and less prone to absorption of hydrophobic molecules. The etching/lamination-based method described here results in a variety of all-PS devices, with or without electrodes and tubing. To characterize the devices, micrographs of etched channels (straight and intersected channels) were taken using confocal and scanning electron microscopy. Microchip-based electrophoresis with repetitive injections of fluorescein was conducted using a three-sided PS (etched pinched, twin-tee channel) and one-sided PDMS device. Microchip-based flow injection analysis, with dopamine and NO as analytes, was used to characterize the performance of all-PS devices with embedded tubing and electrodes. Limits of detection for dopamine and NO were 130 nM and 1.8 μM, respectively. Cell immobilization studies were also conducted to assess all-PS devices for cellular analysis. This paper demonstrates that these easy to fabricate devices can be attractive alternative to other PS fabrication methods for a wide variety of analytical and cell culture applications.

Computational investigation of drug-resistant mutant of M2 proton channel (S31N) against rimantadine.

PubMed

Karthick, V; Ramanathan, K

2014-11-01

M2 proton channel is the target for treating the patients who ere suffering from influenza A infection, which facilitates the spread of virions. Amantadine and rimantadine are adamantadine-based drugs, which target M2 proton channel and inhibit the viral replication. Preferably, rimantadine drug is used more than amantadine because of its fewer side effects. However, S31N mutation in the M2 proton channel was highly resistant to the rimantadine drug. Therefore, in the present study, we focused to understand the drug-resistance mechanism of S31N mutation with the aid of molecular docking and dynamics approach. The docking analysis undoubtedly indicates that affinity for rimantadine with mutant-type M2 proton channel is significantly lesser than the native-type M2 proton channel. In addition, RMSD, RMSF, and principal component analysis suggested that the mutation shows increased flexibility. Furthermore, the intermolecular hydrogen bonds analysis showed that there is a complete loss of hydrogen bonds in the mutant complex. On the whole, we conclude that the intermolecular contact was maintained by D-44, a key residue for stable binding of rimantadine. These findings are certainly helpful for better understanding of drug-resistance mechanism and also helpful for designing new drugs for treating influenza infection against drug-resistance target.

Bipolar Electrode Sample Preparation Devices

NASA Technical Reports Server (NTRS)

Song, Hongjun (Inventor); Wang, Yi (Inventor); Pant, Kapil (Inventor)

2017-01-01

An analyte selection device can include: a body defining a fluid channel having a channel inlet and channel outlet; a bipolar electrode (BPE) between the inlet and outlet; one of an anode or cathode electrically coupled with the BPE on a channel inlet side of the BPE and the other of the anode or cathode electrically coupled with the BPE on a channel outlet side of the BPE; and an electronic system operably coupled with the anode and cathode so as to polarize the BPE. The fluid channel can have any shape or dimension. The channel inlet and channel outlet can be longitudinal or lateral with respect to the longitudinal axis of the channel. The BPE can be any metallic member, such as a flat plate on a wall or mesh as a barrier BPE. The anode and cathode can be located at a position that polarizes the BPE.

Side-channel Analysis of Subscriber Identity Modules

DTIC Science & Technology

2013-06-01

with fuming nitric acid or a similar caustic chemical. Once the die is bare, the problem of extreme miniaturization can be mitigated with the use of...laboratory. Had this possibility been considered earlier, less time would have been spent attempting to work around the probe limitations. 5.3 Future

Electrostatics of aquaporin and aquaglyceroporin channels correlates with their transport selectivity

PubMed Central

Oliva, Romina; Calamita, Giuseppe; Thornton, Janet M.; Pellegrini-Calace, Marialuisa

2010-01-01

Aquaporins are homotetrameric channel proteins, which allow the diffusion of water and small solutes across biological membranes. According to their transport function, aquaporins can be divided into “orthodox aquaporins”, which allow the flux of water molecules only, and “aquaglyceroporins”, which facilitate the diffusion of glycerol and other small solutes in addition to water. The contribution of individual residues in the pore to the selectivity of orthodox aquaporins and aquaglyceroporins is not yet fully understood. To gain insights into aquaporin selectivity, we focused on the sequence variation and electrostatics of their channels. The continuum Poisson-Boltzmann electrostatic potential along the channel was calculated and compared for ten three-dimensional-structures which are representatives of different aquaporin subfamilies, and a panel of functionally characterized mutants, for which high-accuracy three-dimensional-models could be derived. Interestingly, specific electrostatic profiles associated with the main selectivity to water or glycerol could be identified. In particular: (i) orthodox aquaporins showed a distinctive electrostatic potential maximum at the periplasmic side of the channel around the aromatic/Arg (ar/R) constriction site; (ii) aquaporin-0 (AQP0), a mammalian aquaporin with considerably low water permeability, had an additional deep minimum at the cytoplasmic side; (iii) aquaglyceroporins showed a rather flat potential all along the channel; and (iv) the bifunctional protozoan PfAQP had an unusual all negative profile. Evaluation of electrostatics of the mutants, along with a thorough sequence analysis of the aquaporin pore-lining residues, illuminated the contribution of specific residues to the electrostatics of the channels and possibly to their selectivity. PMID:20147624

Pleated metal bipolar assembly

DOEpatents

Wilson, Mahlon S.; Zawodzinski, Christine

2001-01-01

A thin low-cost bipolar plate for an electrochemical cell is formed from a polymer support plate with first flow channels on a first side of the support plate and second flow channels on a second side of the support plate, where the first flow channels and second flow channels have intersecting locations and have a depth effective to form openings through the support plate at the intersecting locations. A first foil of electrically conductive material is pressed into the first flow channels. A second foil of electrically conductive material pressed into the second flow channels so that electrical contact is made between the first and second foils at the openings through the support plate. A particular application of the bipolar plate is in polymer electrolyte fuel cells.

Optimizing the wireless power transfer over MIMO Channels

NASA Astrophysics Data System (ADS)

Wiedmann, Karsten; Weber, Tobias

2017-09-01

In this paper, the optimization of the power transfer over wireless channels having multiple-inputs and multiple-outputs (MIMO) is studied. Therefore, the transmitter, the receiver and the MIMO channel are modeled as multiports. The power transfer efficiency is described by a Rayleigh quotient, which is a function of the channel's scattering parameters and the incident waves from both transmitter and receiver side. This way, the power transfer efficiency can be maximized analytically by solving a generalized eigenvalue problem, which is deduced from the Rayleigh quotient. As a result, the maximum power transfer efficiency achievable over a given MIMO channel is obtained. This maximum can be used as a performance bound in order to benchmark wireless power transfer systems. Furthermore, the optimal operating point which achieves this maximum will be obtained. The optimal operating point will be described by the complex amplitudes of the optimal incident and reflected waves of the MIMO channel. This supports the design of the optimal transmitter and receiver multiports. The proposed method applies for arbitrary MIMO channels, taking transmitter-side and/or receiver-side cross-couplings in both near- and farfield scenarios into consideration. Special cases are briefly discussed in this paper in order to illustrate the method.

Hydrogen-terminated diamond vertical-type metal oxide semiconductor field-effect transistors with a trench gate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Inaba, Masafumi, E-mail: inaba-ma@ruri.waseda.jp; Muta, Tsubasa; Kobayashi, Mikinori

2016-07-18

The hydrogen-terminated diamond surface (C-H diamond) has a two-dimensional hole gas (2DHG) layer independent of the crystal orientation. A 2DHG layer is ubiquitously formed on the C-H diamond surface covered by atomic-layer-deposited-Al{sub 2}O{sub 3}. Using Al{sub 2}O{sub 3} as a gate oxide, C-H diamond metal oxide semiconductor field-effect transistors (MOSFETs) operate in a trench gate structure where the diamond side-wall acts as a channel. MOSFETs with a side-wall channel exhibit equivalent performance to the lateral C-H diamond MOSFET without a side-wall channel. Here, a vertical-type MOSFET with a drain on the bottom is demonstrated in diamond with channel current modulationmore » by the gate and pinch off.« less

Detector-device-independent quantum key distribution: Security analysis and fast implementation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boaron, Alberto; Korzh, Boris; Boso, Gianluca

One of the most pressing issues in quantum key distribution (QKD) is the problem of detector side-channel attacks. To overcome this problem, researchers proposed an elegant “time-reversal” QKD protocol called measurement-device-independent QKD (MDI-QKD), which is based on time-reversed entanglement swapping. However, MDI-QKD is more challenging to implement than standard point-to-point QKD. Recently, an intermediary QKD protocol called detector-device-independent QKD (DDI-QKD) has been proposed to overcome the drawbacks of MDI-QKD, with the hope that it would eventually lead to a more efficient detector side-channel-free QKD system. Here, we analyze the security of DDI-QKD and elucidate its security assumptions. We find thatmore » DDI-QKD is not equivalent to MDI-QKD, but its security can be demonstrated with reasonable assumptions. On the more practical side, we consider the feasibility of DDI-QKD and present a fast experimental demonstration (clocked at 625 MHz), capable of secret key exchange up to more than 90 km.« less

In-line optofluidic refractive index sensing in a side-channel photonic crystal fiber.

PubMed

Zhang, Nan; Humbert, Georges; Wu, Zhifang; Li, Kaiwei; Shum, Perry Ping; Zhang, Nancy Meng Ying; Cui, Ying; Auguste, Jean-Louis; Dinh, Xuan Quyen; Wei, Lei

2016-11-28

An in-line optofluidic refractive index (RI) sensing platform is constructed by splicing a side-channel photonic crystal fiber (SC-PCF) with side-polished single mode fibers. A long-period grating (LPG) combined with an intermodal interference between LP₀₁ and LP₁₁ core modes is used for sensing the RI of the liquid in the side channel. The resonant dip shows a nonlinear wavelength shift with increasing RI over the measured range from 1.3330 to 1.3961. The RI response of this sensing platform for a low RI range of 1.3330-1.3780 is approximately linear, and exhibits a sensitivity of 1145 nm/RIU. Besides, the detection limit of our sensing scheme is improved by around one order of magnitude by introducing the intermodal interference.

Fluvial processes and passive rehabilitation of the Lisbon Bottom side-channel chute, Lower Missouri River

USGS Publications Warehouse

Jacobson, Robert B.; Laustrup, Mark S.; Chapman, Michael D.

2001-01-01

Multiple large floods in 1993-1997 on the Lower Missouri River carved a side-channel chute through the river bottom at Lisbon, Missouri. Although similar in some respects to engineered side-channel chutes designed for habitat rehabilitation projects, the Lisbon Bottom chute has been unique in that it was allowed to evolve for more than four years with minimal stabilization. During the wet years, 1996-1999, the chute was subjected to abnormally high discharges and passed as much as 20% of the total discharge of the Missouri River. Relatively unrestrained fluvial processes during this time created a wide channel with highly diverse habitats. The upper one-half of the chute established a shallow, braided channel morphology similar to the pre-managed Missouri River. The lower half established a dynamically migrating, single-thread channel, and an incipient flood plain. Compared to the adjacent navigation channel, the chute established substantial areas of shallow, slow-velocity aquatic habitat that is considered to be in short supply in the present-day Lower Missouri River. The shortterm biological benefits have been mixed: the chute has fewer waterbird and benthic macroinvertebrate taxa than adjacent riverine habitats, but greater numbers of fish species compared to the navigation channel.

Measurement of heat transfer and pressure drop in rectangular channels with turbulence promoters

NASA Technical Reports Server (NTRS)

Han, J. C.; Park, J. S.; Ibrahim, M. Y.

1986-01-01

Periodic rib turbulators were used in advanced turbine cooling designs to enhance the internal heat transfer. The objective of the present project was to investigate the combined effects of the rib angle of attack and the channel aspect ratio on the local heat transfer and pressure drop in rectangular channels with two opposite ribbed walls for Reynolds number varied from 10,000 to 60,000. The channel aspect ratio (W/H) was varied from 1 to 2 to 4. The rib angle of attack (alpha) was varied from 90 to 60 to 45 to 30 degree. The highly detailed heat transfer coefficient distribution on both the smooth side and the ribbed side walls from the channel sharp entrance to the downstream region were measured. The results showed that, in the square channel, the heat transfer for the slant ribs (alpha = 30 -45 deg) was about 30% higher that of the transverse ribs (alpha = 90 deg) for a constant pumping power. However, in the rectangular channels (W/H = 2 and 4, ribs on W side), the heat transfer at alpha = 30 -45 deg was only about 5% higher than 90 deg. The average heat transfer and friction correlations were developed to account for rib spacing, rib angle, and channel aspect ratio over the range of roughness Reynolds number.

A structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels

PubMed Central

Napolitano, Luisa Maria Rosaria; Bisha, Ina; De March, Matteo; Marchesi, Arin; Arcangeletti, Manuel; Demitri, Nicola; Mazzolini, Monica; Rodriguez, Alex; Magistrato, Alessandra; Onesti, Silvia; Laio, Alessandro; Torre, Vincent

2015-01-01

Cyclic nucleotide-gated (CNG) ion channels, despite a significant homology with the highly selective K+ channels, do not discriminate among monovalent alkali cations and are permeable also to several organic cations. We combined electrophysiology, molecular dynamics (MD) simulations, and X-ray crystallography to demonstrate that the pore of CNG channels is highly flexible. When a CNG mimic is crystallized in the presence of a variety of monovalent cations, including Na+, Cs+, and dimethylammonium (DMA+), the side chain of Glu66 in the selectivity filter shows multiple conformations and the diameter of the pore changes significantly. MD simulations indicate that Glu66 and the prolines in the outer vestibule undergo large fluctuations, which are modulated by the ionic species and the voltage. This flexibility underlies the coupling between gating and permeation and the poor ionic selectivity of CNG channels. PMID:26100907

A structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels.

PubMed

Napolitano, Luisa Maria Rosaria; Bisha, Ina; De March, Matteo; Marchesi, Arin; Arcangeletti, Manuel; Demitri, Nicola; Mazzolini, Monica; Rodriguez, Alex; Magistrato, Alessandra; Onesti, Silvia; Laio, Alessandro; Torre, Vincent

2015-07-07

Cyclic nucleotide-gated (CNG) ion channels, despite a significant homology with the highly selective K(+) channels, do not discriminate among monovalent alkali cations and are permeable also to several organic cations. We combined electrophysiology, molecular dynamics (MD) simulations, and X-ray crystallography to demonstrate that the pore of CNG channels is highly flexible. When a CNG mimic is crystallized in the presence of a variety of monovalent cations, including Na(+), Cs(+), and dimethylammonium (DMA(+)), the side chain of Glu66 in the selectivity filter shows multiple conformations and the diameter of the pore changes significantly. MD simulations indicate that Glu66 and the prolines in the outer vestibule undergo large fluctuations, which are modulated by the ionic species and the voltage. This flexibility underlies the coupling between gating and permeation and the poor ionic selectivity of CNG channels.

Influence of the elastic deformation of a foam on its mobility in channels of linearly varying width.

PubMed

Dollet, Benjamin; Jones, Siân A; Méheust, Yves; Cantat, Isabelle

2014-08-01

We study foam flow in an elementary model porous medium consisting of a convergent and a divergent channel positioned side by side and possessing a fixed joint porosity. Configurations of converging or diverging channels are ubiquitous at the pore scale in porous media, as all channels linking pores possess a converging and diverging part. The resulting flow kinematics imposes asymmetric bubble deformations in the two channels, which modulate foam-wall friction and strongly impact the flux distribution. We measure, as well as quantitatively predict, the ratio of the fluxes in the two channels as a function of the channel widths by modeling pressure drops of both viscous and capillary origins. This study reveals the crucial importance of boundary-induced bubble deformation on the mobility of a flowing foam, resulting in particular in flow irreversibility.

Initial processing and analysis of forward- and side-looking data from the Spectrally Agile Frequency-Incrementing Reconfigurable (SAFIRE) radar

NASA Astrophysics Data System (ADS)

Ranney, Kenneth; Phelan, Brian; Sherbondy, Kelly; Kirose, Getachew; Smith, Gregory; Clark, John; Harrison, Arthur; Ressler, Marc; Nguyen, Lam; Narayanan, Ram

2017-05-01

A new, versatile, UHF/L band, ultrawideband (UWB), vehicle-mounted radar system developed at the U.S. Army Research Laboratory (ARL) has recently been exercised at an arid U.S. test site. The unique switching scheme implemented to record data from all receive channels is described, along with the current calibration procedure. Radar and global positioning system (GPS) data collected in both forwardand side-looking configurations are processed, and synthetic aperture radar (SAR) images are formed. Results are presented for various target emplacement scenarios.

Convective heat transfer in a high aspect ratio minichannel heated on one side

DOE PAGES

Forrest, Eric C.; Hu, Lin -Wen; Buongiorno, Jacopo; ...

2015-10-21

Experimental results are presented for single-phase heat transfer in a narrow rectangular minichannel heated on one side. The aspect ratio and gap thickness of the test channel were 29:1 and 1.96 mm, respectively. Friction pressure drop and Nusselt numbers are reported for the transition and fully turbulent flow regimes, with Prandtl numbers ranging from 2.2 to 5.4. Turbulent friction pressure drop for the high aspect ratio channel is well-correlated by the Blasius solution when a modified Reynolds number, based upon a laminar equivalent diameter, is utilized. The critical Reynolds number for the channel falls between 3500 and 4000, with Nusseltmore » numbers in the transition regime being reasonably predicted by Gnielinski's correlation. The dependence of the heat transfer coefficient on the Prandtl number is larger than that predicted by circular tube correlations, and is likely a result of the asymmetric heating. The problem of asymmetric heating condition is approached theoretically using a boundary layer analysis with a two-region wall layer model, similar to that originally proposed by Prandtl. The analysis clarifies the influence of asymmetric heating on the Nusselt number and correctly predicts the experimentally observed trend with Prandtl number. Furthermore, a semi-analytic correlation is derived from the analysis that accounts for the effect of aspect ratio and asymmetric heating, and is shown to predict the experimental results of this study with a mean absolute error (MAE) of less than 5% for 4000 < Re < 70,000.« less

McEliece PKC Calculator

NASA Astrophysics Data System (ADS)

Marek, Repka

2015-01-01

The original McEliece PKC proposal is interesting thanks to its resistance against all known attacks, even using quantum cryptanalysis, in an IND-CCA2 secure conversion. Here we present a generic implementation of the original McEliece PKC proposal, which provides test vectors (for all important intermediate results), and also in which a measurement tool for side-channel analysis is employed. To our best knowledge, this is the first such an implementation. This Calculator is valuable in implementation optimization, in further McEliece/Niederreiter like PKCs properties investigations, and also in teaching. Thanks to that, one can, for example, examine side-channel vulnerability of a certain implementation, or one can find out and test particular parameters of the cryptosystem in order to make them appropriate for an efficient hardware implementation. This implementation is available [1] in executable binary format, and as a static C++ library, as well as in form of source codes, for Linux and Windows operating systems.

Fuel cell membrane humidification

DOEpatents

Wilson, Mahlon S.

1999-01-01

A polymer electrolyte membrane fuel cell assembly has an anode side and a cathode side separated by the membrane and generating electrical current by electrochemical reactions between a fuel gas and an oxidant. The anode side comprises a hydrophobic gas diffusion backing contacting one side of the membrane and having hydrophilic areas therein for providing liquid water directly to the one side of the membrane through the hydrophilic areas of the gas diffusion backing. In a preferred embodiment, the hydrophilic areas of the gas diffusion backing are formed by sewing a hydrophilic thread through the backing. Liquid water is distributed over the gas diffusion backing in distribution channels that are separate from the fuel distribution channels.

Reaction mechanism of dimethyl ether carbonylation to methyl acetate over mordenite – a combined DFT/experimental study

DOE PAGES

Rasmussen, D. B.; Christensen, J. M.; Temel, B.; ...

2017-01-23

The reaction mechanism of dimethyl ether carbonylation to methyl acetate over mordenite was studied theoretically with periodic density functional theory calculations including dispersion forces and experimentally in a fixed bed flow reactor at pressures between 10 and 100 bar, dimethyl ether concentrations in CO between 0.2 and 2.0%, and at a temperature of 438 K. The theoretical study showed that the reaction of CO with surface methyl groups, the rate-limiting step, is faster in the eight-membered side pockets than in the twelve-membered main channel of the zeolite; the subsequent reaction of dimethyl ether with surface acetyl to form methyl acetatemore » was demonstrated to occur with low energy barriers in both the side pockets and in the main channel. Here, the present analysis has thus identified a path, where the entire reaction occurs favourably on a single site within the side pocket, in good agreement with previous experimental studies. The experimental study of the reaction kinetics was consistent with the theoretically derived mechanism and in addition revealed that the methyl acetate product inhibits the reaction – possibly by sterically hindering the attack of CO on the methyl groups in the side pockets.« less

Software and Critical Technology Protection Against Side-Channel Analysis Through Dynamic Hardware Obfuscation

DTIC Science & Technology

2011-03-01

resampling a second time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 70 Plot of RSA bitgroup exponentiation with DAILMOM after a...14 DVFS Dynamic Voltage and Frequency Switching . . . . . . . . . . . . . . . . . . . 14 MDPL Masked Dual-Rail...algorithms to prevent whole-sale discovery of PINs and other simple methods to prevent employee tampering [5]. In time , cryptographic systems have

Permeation of internal and external monovalent cations through the catfish cone photoreceptor cGMP-gated channel

PubMed Central

1995-01-01

The permeation of monovalent cations through the cGMP-gated channel of catfish cone outer segments was examined by measuring permeability and conductance ratios under biionic conditions. For monovalent cations presented on the cytoplasmic side of the channel, the permeability ratios with respect to extracellular Na followed the sequence NH4 > K > Li > Rb = Na > Cs while the conductance ratios at +50 mV followed the sequence Na approximately NH4 > K > Rb > Li = Cs. These patterns are broadly similar to the amphibian rod channel. The symmetry of the channel was tested by presenting the test ion on the extracellular side and using Na as the common reference ion on the cytoplasmic side. Under these biionic conditions, the permeability ratios with respect to Na at the intracellular side followed the sequence NH4 > Li > K > Na > Rb > Cs while the conductance ratios at +50 mV followed the sequence NH4 > K approximately Na > Rb > Li > Cs. Thus, the channel is asymmetric with respect to external and internal cations. Under symmetrical 120 mM ionic conditions, the single-channel conductance at +50 mV ranged from 58 pS in NH4 to 15 pS for Cs and was in the order NH4 > Na > K > Rb > Cs. Unexpectedly, the single-channel current-voltage relation showed sufficient outward rectification to account for the rectification observed in multichannel patches without invoking voltage dependence in gating. The concentration dependence of the reversal potential for K showed that chloride was impermeant. Anomalous mole fraction behavior was not observed, nor, over a limited concentration range, were multiple dissociation constants. An Eyring rate theory model with a single binding site was sufficient to explain these observations. PMID:8786344

Towards a Basis for Designing Backwater and Side Channel Restorations

USDA-ARS?s Scientific Manuscript database

Design criteria for river channel restoration is becoming highly developed with several handbooks and guidance documents available, despite notable differences among various schools of thought. Basic principles of stable channel design and riverine habitat simulation undergird channel reconstructio...

The comprehensive analysis of DEG/ENaC subunits in Hydra reveals a large variety of peptide-gated channels, potentially involved in neuromuscular transmission.

PubMed

Assmann, Marc; Kuhn, Anne; Dürrnagel, Stefan; Holstein, Thomas W; Gründer, Stefan

2014-10-14

It is generally the case that fast transmission at neural synapses is mediated by small molecule neurotransmitters. The simple nervous system of the cnidarian Hydra, however, contains a large repertoire of neuropeptides and it has been suggested that neuropeptides are the principal transmitters of Hydra. An ion channel directly gated by Hydra-RFamide neuropeptides has indeed been identified in Hydra - the Hydra Na+ channel (HyNaC) 2/3/5, which is expressed at the oral side of the tentacle base. Hydra-RFamides are more widely expressed, however, being found in neurons of the head and peduncle region. Here, we explore whether further peptide-gated HyNaCs exist, where in the animal they are expressed, and whether they are all gated by Hydra-RFamides. We report molecular cloning of seven new HyNaC subunits - HyNaC6 to HyNaC12, all of which are members of the DEG/ENaC gene family. In Xenopus oocytes, these subunits assemble together with the four already known subunits into thirteen different ion channels that are directly gated by Hydra-RFamide neuropeptides with high affinity (up to 40 nM). In situ hybridization suggests that HyNaCs are expressed in epitheliomuscular cells at the oral and the aboral side of the tentacle base and at the peduncle. Moreover, diminazene, an inhibitor of HyNaCs, delayed tentacle movement in live Hydra. Our results show that Hydra has a large variety of peptide-gated ion channels that are activated by a restricted number of related neuropeptides. The existence and expression pattern of these channels, and behavioral effects induced by channel blockers, suggests that Hydra co-opted neuropeptides for fast neuromuscular transmission.

Crystal Structure of a Mammalian Voltage-Dependent Shaker Family K+ Channel

NASA Astrophysics Data System (ADS)

Long, Stephen B.; Campbell, Ernest B.; MacKinnon, Roderick

2005-08-01

Voltage-dependent potassium ion (K+) channels (Kv channels) conduct K+ ions across the cell membrane in response to changes in the membrane voltage, thereby regulating neuronal excitability by modulating the shape and frequency of action potentials. Here we report the crystal structure, at a resolution of 2.9 angstroms, of a mammalian Kv channel, Kv1.2, which is a member of the Shaker K+ channel family. This structure is in complex with an oxido-reductase β subunit of the kind that can regulate mammalian Kv channels in their native cell environment. The activation gate of the pore is open. Large side portals communicate between the pore and the cytoplasm. Electrostatic properties of the side portals and positions of the T1 domain and β subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the β subunit.

Delineating forested river habitats and riparian floodplain hydrology with LiDAR

NASA Astrophysics Data System (ADS)

Vondrasek, Chris

Rivers and the riparian forest corridor comprise a valuable freshwater ecosystem that has been altered by human activities including timber management, road building, and other land conversions. The habitats of river dependent species in the Pacific Northwest, in particular salmon have often been degraded by these activities. Many salmon runs have become threatened with extinction and have been Endangered Species Act listed. New conservation planning and policies have developed around protecting freshwater habitats and restoring more natural river processes. In WA State, timber landowners, officials from State and Federal agencies, Native tribes, and other stakeholders developed Forest Practice rules and codified a Habitat Conservation Plan with dual goals of providing regulatory surety for timber land owners and helping to recover the threatened salmon runs in forested watersheds. Conserving critical stream ecological functions and potential fish habitats throughout watersheds while managing and regulating timber harvest across the State requires accurate and up-to-date delineation and mapping of channels, tributaries, and off-channel wetlands. Monitoring the effectiveness of protection efforts is necessary but can also be difficult. Agency staff and resources are limited for both day-to-day implementation of Forest Practice rules and adaptive management. The goal of this research has been to develop efficient and accessible methods to delineate wetlands, side-channels, tributaries, and pools and backwaters created by large log jams in forested watersheds. It was also essential to use publicly available LiDAR data and to model these waters at ecologically meaningful flows. I tested a hydraulic model at a 2-year and 50-year flows, and a relative height above river surface model and compared them. I completed two additional remote sensing investigations to correlate channel movement and the locations of off-channel wetlands: an analysis of historical aerial imagery and models of the riparian forest tree establishment using the first-return lidar data. The research includes two fieldwork components: an appraisal of the delineated off-channel and active channel water features, and an assessment of the accuracy of the lidar under the forest canopy. Both the hydraulic and the relative elevation models accurately delineated the key off-channel and active channel waters. The historical imagery analysis confirmed past channel movement left many of the side channels and wetlands near to the contemporary active channel. The sequence of tree establishment tracked where channel migration had exposed new banks, colonized first by deciduous trees, then followed by cohorts of conifers, some maturing and achieving great heights. Often the lack of a closed canopy corresponded to the locations of persistent wetlands or mid-channel logjams Key Words: Floodplain hydrology, channel movement, wetlands, off-channel habitats, habitat conservation plans, hydraulic models, lidar, historical imagery, riparian forest establishment.

Fuel cell assembly unit for promoting fluid service and electrical conductivity

DOEpatents

Jones, Daniel O.

1999-01-01

Fluid service and/or electrical conductivity for a fuel cell assembly is promoted. Open-faced flow channel(s) are formed in a flow field plate face, and extend in the flow field plate face between entry and exit fluid manifolds. A resilient gas diffusion layer is located between the flow field plate face and a membrane electrode assembly, fluidly serviced with the open-faced flow channel(s). The resilient gas diffusion layer is restrained against entering the open-faced flow channel(s) under a compressive force applied to the fuel cell assembly. In particular, a first side of a support member abuts the flow field plate face, and a second side of the support member abuts the resilient gas diffusion layer. The support member is formed with a plurality of openings extending between the first and second sides of the support member. In addition, a clamping pressure is maintained for an interface between the resilient gas diffusion layer and a portion of the membrane electrode assembly. Preferably, the support member is spikeless and/or substantially flat. Further, the support member is formed with an electrical path for conducting current between the resilient gas diffusion layer and position(s) on the flow field plate face.

Fe(2+) substrate transport through ferritin protein cage ion channels influences enzyme activity and biomineralization.

PubMed

Behera, Rabindra K; Torres, Rodrigo; Tosha, Takehiko; Bradley, Justin M; Goulding, Celia W; Theil, Elizabeth C

2015-09-01

Ferritins, complex protein nanocages, form internal iron-oxy minerals (Fe2O3·H2O), by moving cytoplasmic Fe(2+) through intracage ion channels to cage-embedded enzyme (2Fe(2+)/O2 oxidoreductase) sites where ferritin biomineralization is initiated. The products of ferritin enzyme activity are diferric oxy complexes that are mineral precursors. Conserved, carboxylate amino acid side chains of D127 from each of three cage subunits project into ferritin ion channels near the interior ion channel exits and, thus, could direct Fe(2+) movement to the internal enzyme sites. Ferritin D127E was designed and analyzed to probe properties of ion channel size and carboxylate crowding near the internal ion channel opening. Glu side chains are chemically equivalent to, but longer by one -CH2 than Asp, side chains. Ferritin D127E assembled into normal protein cages, but diferric peroxo formation (enzyme activity) was not observed, when measured at 650 nm (DFP λ max). The caged biomineral formation, measured at 350 nm in the middle of the broad, nonspecific Fe(3+)-O absorption band, was slower. Structural differences (protein X-ray crystallography), between ion channels in wild type and ferritin D127E, which correlate with the inhibition of ferritin D127E enzyme activity include: (1) narrower interior ion channel openings/pores; (2) increased numbers of ion channel protein-metal binding sites, and (3) a change in ion channel electrostatics due to carboxylate crowding. The contributions of ion channel size and structure to ferritin activity reflect metal ion transport in ion channels are precisely regulated both in ferritin protein nanocages and membranes of living cells.

Fe2+ Substrate Transport through Ferritin Protein Cage Ion Channels Influences Enzyme Activity and Biomineralization

PubMed Central

Behera, Rabindra K.; Torres, Rodrigo; Tosha, Takehiko; Bradley, Justin M.; Goulding, Celia W.; Theil, Elizabeth C.

2015-01-01

Ferritins, complex protein nanocages, form internal iron-oxy minerals (Fe2O3.H2O), by moving cytoplasmic Fe2+ through intracage ion channels to cage-embedded enzyme (2Fe2+/O2 oxidoreductase) sites where ferritin biomineralization is initiated. The products of ferritin enzyme activity are diferric oxy complexes that are mineral precursors. Conserved, carboxylate amino acid side chains of D127 from each of three cage subunits project into ferritin ion channels near the interior ion channel exits and, thus, could direct Fe2+ movement to the internal enzyme sites. Ferritin D127E was designed and analyzed to probe properties of ion channel size and carboxylate crowding near the internal ion channel opening. Glu side chains are chemically equivalent to, but longer by one – CH2 than Asp, side chains. Ferritin D127E assembled into normal protein cages, but diferric peroxo formation (enzyme activity) was not observed, when measured at 650nm (DFP λmax). The caged biomineral formation, measured at 350 nm in the middle of the broad, nonspecific Fe3+-O absorption band, was slower. Structural differences (protein X-ray crystallography), between ion channels in wild type and ferritin D127E, which correlate with the inhibition of ferritin D127E enzyme activity include: 1. narrower interior ion channel openings/pores, 2. increased numbers of ion channel protein-metal binding sites, and 3. a change in ion channel electrostatics due to carboxylate crowding. The contributions of ion channel size and structure to ferritin activity reflect metal ion transport in ion channels are precisely regulated both in ferritin protein nanocages and membranes of living cells. PMID:26202907

Zebrafish CaV2.1 Calcium Channels Are Tailored for Fast Synchronous Neuromuscular Transmission

PubMed Central

Naranjo, David; Wen, Hua; Brehm, Paul

2015-01-01

The CaV2.2 (N-type) and CaV2.1 (P/Q-type) voltage-dependent calcium channels are prevalent throughout the nervous system where they mediate synaptic transmission, but the basis for the selective presence at individual synapses still remains an open question. The CaV2.1 channels have been proposed to respond more effectively to brief action potentials (APs), an idea supported by computational modeling. However, the side-by-side comparison of CaV2.1 and CaV2.2 kinetics in intact neurons failed to reveal differences. As an alternative means for direct functional comparison we expressed zebrafish CaV2.1 and CaV2.2 α-subunits, along with their accessory subunits, in HEK293 cells. HEK cells lack calcium currents, thereby circumventing the need for pharmacological inhibition of mixed calcium channel isoforms present in neurons. HEK cells also have a simplified morphology compared to neurons, which improves voltage control. Our measurements revealed faster kinetics and shallower voltage-dependence of activation and deactivation for CaV2.1. Additionally, recordings of calcium current in response to a command waveform based on the motorneuron AP show, directly, more effective activation of CaV2.1. Analysis of calcium currents associated with the AP waveform indicate an approximately fourfold greater open probability (PO) for CaV2.1. The efficient activation of CaV2.1 channels during APs may contribute to the highly reliable transmission at zebrafish neuromuscular junctions. PMID:25650925

Identifying the elusive link between amino acid sequence and charge selectivity in pentameric ligand-gated ion channels.

PubMed

Cymes, Gisela D; Grosman, Claudio

2016-10-10

Among neurotransmitter-gated ion channels, the superfamily of pentameric ligand-gated ion channels (pLGICs) is unique in that its members display opposite permeant-ion charge selectivities despite sharing the same structural fold. Although much effort has been devoted to the identification of the mechanism underlying the cation-versus-anion selectivity of these channels, a careful analysis of past work reveals that discrepancies exist, that different explanations for the same phenomenon have often been put forth, and that no consensus view has yet been reached. To elucidate the molecular basis of charge selectivity for the superfamily as a whole, we performed extensive mutagenesis and electrophysiological recordings on six different cation-selective and anion-selective homologs from vertebrate, invertebrate, and bacterial origin. We present compelling evidence for the critical involvement of ionized side chains-whether pore-facing or buried-rather than backbone atoms and propose a mechanism whereby not only their charge sign but also their conformation determines charge selectivity. Insertions, deletions, and residue-to-residue mutations involving nonionizable residues in the intracellular end of the pore seem to affect charge selectivity by changing the rotamer preferences of the ionized side chains in the first turn of the M2 α-helices. We also found that, upon neutralization of the charged residues in the first turn of M2, the control of charge selectivity is handed over to the many other ionized side chains that decorate the pore. This explains the long-standing puzzle as to why the neutralization of the intracellular-mouth glutamates affects charge selectivity to markedly different extents in different cation-selective pLGICs.

MULTI-SCALE MODELING AND APPROXIMATION ASSISTED OPTIMIZATION OF BARE TUBE HEAT EXCHANGERS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bacellar, Daniel; Ling, Jiazhen; Aute, Vikrant

2014-01-01

Air-to-refrigerant heat exchangers are very common in air-conditioning, heat pump and refrigeration applications. In these heat exchangers, there is a great benefit in terms of size, weight, refrigerant charge and heat transfer coefficient, by moving from conventional channel sizes (~ 9mm) to smaller channel sizes (< 5mm). This work investigates new designs for air-to-refrigerant heat exchangers with tube outer diameter ranging from 0.5 to 2.0mm. The goal of this research is to develop and optimize the design of these heat exchangers and compare their performance with existing state of the art designs. The air-side performance of various tube bundle configurationsmore » are analyzed using a Parallel Parameterized CFD (PPCFD) technique. PPCFD allows for fast-parametric CFD analyses of various geometries with topology change. Approximation techniques drastically reduce the number of CFD evaluations required during optimization. Maximum Entropy Design method is used for sampling and Kriging method is used for metamodeling. Metamodels are developed for the air-side heat transfer coefficients and pressure drop as a function of tube-bundle dimensions and air velocity. The metamodels are then integrated with an air-to-refrigerant heat exchanger design code. This integration allows a multi-scale analysis of air-side performance heat exchangers including air-to-refrigerant heat transfer and phase change. Overall optimization is carried out using a multi-objective genetic algorithm. The optimal designs found can exhibit 50 percent size reduction, 75 percent decrease in air side pressure drop and doubled air heat transfer coefficients compared to a high performance compact micro channel heat exchanger with same capacity and flow rates.« less

Secular bathymetric variations of the North Channel in the Changjiang (Yangtze) Estuary, China, 1880-2013: Causes and effects

NASA Astrophysics Data System (ADS)

Mei, Xuefei; Dai, Zhijun; Wei, Wen; Li, Weihua; Wang, Jie; Sheng, Hao

2018-02-01

As the interface between the fluvial upland system and the open coast, global estuaries are facing serious challenges owing to various anthropogenic activities, especially to the Changjiang Estuary. Since the establishment of the Three Gorges Dam (TGD), currently the world's largest hydraulic structure, and certain other local hydraulic engineering structures, the Changjiang Estuary has experienced severe bathymetric variations. It is urgent to analyze the estuarine morphological response to the basin-wide disturbance to enable a better management of estuarine environments. North Channel (NC), the largest anabranched estuary in the Changjiang Estuary, is the focus of this study. Based on the analysis of bathymetric data between 1880 and 2013 and related hydrological data, we developed the first study on the centennial bathymetric variations of the NC. It is found that the bathymetric changes of NC include two main modes, with the first mode representing 64% of the NC variability, which indicates observable deposition in the mouth bar and its outer side area (lower reach); the second mode representing 11% of the NC variability, which further demonstrates channel deepening along the inner side of the mouth bar (upper reach) during 1970-2013. Further, recent erosion observed along the inner side of the mouth bar is caused by riverine sediment decrease, especially in relation to TGD induced sediment trapping since 2003, while the deposition along the lower reach since 2003 can be explained by the landward sediment transport because of flood-tide force strengthen under the joint action of TGD induced seasonal flood discharge decrease and land reclamation induced lower reach narrowing. Generally, the upper and lower NC reach are respectively dominated by fluvial and tidal discharge, however, episodic extreme floods can completely alter the channel morphology by smoothing the entire channel. The results presented herein for the NC enrich our understanding of bathymetric variations of the Changjiang Estuary in response to human activities, which can be well applied to other estuaries subject to similar interferences.

Spatial analysis of muscular activations in stroke survivors.

PubMed

Rasool, Ghulam; Afsharipour, Babak; Suresh, Nina L; Xiaogang Hu; Rymer, William Zev

2015-01-01

We investigated the spatial patterns of electrical activity in stroke-affected muscles using the high density surface electromyogram (sEMG) grids. We acquired 128-channel sEMG signals from the impaired as well as contralateral Biceps Brachii (BB) muscles of stroke survivors and from healthy participants at various force levels from 20 to 60% of maximum voluntary contraction in an isometric non-fatiguing recording protocol. We found the spatial sEMG pattern to be consistent across force levels in healthy and stroke subjects. However, once compared across sides (left vs right in healthy and impaired vs. contralateral in stroke) we found stroke-affected sides to be significantly different in distribution pattern of sEMG from the contralateral side. The sEMG activity areas were significantly shrunk on the affected sides indicating muscle atrophy due to stroke.

A Sediment Transport Based Geomorphic Analysis of the Skykomish River Braided Reach to Identify "Restoration" Opportunities

NASA Astrophysics Data System (ADS)

Devries, P.; Aldrich, R.; Brunzell, S.; Purser, M.

2004-12-01

A study is underway to assess the driving sediment transport and hydraulic processes influencing channel changes in a braided reach of the Skykomish River, Washington. The reach is located below a steeper, confined section of the river and has likely been geomorphically active since the last glaciation. Bankfull widths range between roughly 100-350 m within, and drainage area is 1,500 km2 above, the study reach. Analyses have been conducted at the reach scale, and include development of a sediment transport model, historical photograph and survey overlays, and an accounting of where sediment deposition, channel shifts and avulsions, and side channel connection are most and least likely to occur over reasonable design life spans (e.g., between 10 and 50 years). The ultimate goal of the analysis is to identify suitable locations for projects that will enhance, restore, or protect fish habitat as well as protect infrastructure, while considering constraints posed by channel hydraulic, sediment transport/deposition, and stability characteristics. Our strategy is to determine what types of projects are best suited for different locations in the reach based on the analysis results. The results can then be used to prioritize and estimate costs for project alternatives.

Transient bedrock channel evolution across a precipitation gradient: A case study from Kohala, Hawaii.

NASA Astrophysics Data System (ADS)

Gasparini, N. M.; Han, J.; Johnson, J. P.; Menking, J. A.

2011-12-01

This study uses observations from the Kohala Peninsula, on the Big Island of Hawaii, and numerical modeling, to explore how precipitation gradients may affect fluvial bedrock incision and channel morphology. Orographic precipitation patterns result in over 4 m/yr of rainfall on the wet side of the peninsula and less than 0.5 m/yr on the dry side. These precipitation patterns likely strongly contribute to the observed channel morphology. Further, the region is subsiding, leading to prolonged transient channel evolution. We explore changes in a number of channel morphologic parameters with watershed averaged precipitation rate. We use PRISM precipitation data and data from isohyets developed from historic rain gauge data. Not surprisingly, valley depth, measured from a 10 meter DEM, increases with spatially averaged precipitation rate. We also find that channel profile form varies with precipitation rate, with drier channels exhibiting a straight to slightly concave channel form and wetter channels exhibiting a convex to concave channel form. The precipitation value at which this transition in channel profile form occurs depends on the precipitation data-set used, highlighting the need for more accurate measurements of precipitation in settings with extreme precipitation patterns similar to our study area. The downstream pattern in precipitation is likely significant in the development of the convex-concave profile form. Numerical modeling results support that precipitation patterns such as those observed on the wet-side of the Kohala Peninsula may contribute to the convex-concave profile form. However, we emphasize that while precipitation patterns may contribute to the channel form, these channel features are transient and not expected to be sustained in steady-state landscapes. We also emphasize that it is fluvial discharge, as driven by precipitation, rather than precipitation alone, that drives the processes shaping the channel form. Because fluvial discharge is integrative, relatively extreme precipitation gradients are required to produce anomalous channel profile forms.

Determining the Side Channel Area in the Ciliwung Watershed for Decreasing the Hydrograph Flood

NASA Astrophysics Data System (ADS)

Yayuk Supomo, Fani; Saleh Pallu, Muh.; Arsyad Thaha, Muh.; Tahir Lopa, Rita

2018-04-01

The condition of Jakarta with high population density and green open space switch function, causing the condition of flooding to be one of the risks that occur when the rainy season. Ciliwung River that flows from Katulampa into Jakarta bay, is considered as the largest contributor to flood discharge. This study will analyze the flood discharge plan on the side channel area to lower the flood hydrograph peaks and extend the detention time. The area to be side channel is Ciparigi with an area of 608.7 hectare and the slope of 8-10%. The result of flood discharge planning analysis at Ciparigi region (Sub watershed of Middle Ciliwung), obtained the amount of flood discharge for return period 2 yearly equal to 10.10 m3/sec, 5 yearly equal to 12.77 m3/sec, 10 yearly equal to 14.17 m3/sec, 25 yearly equal to 15.32 m3/sec, 50 yearly equal to 16.63 m3/sec and 100 yearly equal to 17.52 m3/sec. The percentage of flood discharge plans that will be reduced by 10% of the total flood discharge plan in sub watershed observation is 1.28 m3/sec with reservoir volume of 4.608 m3 which will be fully charged for 1 hour. This will extend the flow time from the control point in Depok to the Manggarai waterway to approximately 5 hours.

Fluidic Oscillator Array for Synchronized Oscillating Jet Generation

NASA Technical Reports Server (NTRS)

Koklu, Mehti (Inventor)

2017-01-01

A fluidic oscillator array includes a plurality of fluidic-oscillator main flow channels. Each main flow channel has an inlet and an outlet. Each main flow channel has first and second control ports disposed at opposing sides thereof, and has a first and a second feedback ports disposed at opposing sides thereof. The feedback ports are located downstream of the control ports with respect to a direction of a fluid flow through the main flow channel. The system also includes a first fluid accumulator in fluid communication with each first control port and each first feedback port, and a second fluid accumulator in fluid communication with each second control port and each second feedback port.

Fluidic Oscillator Array for Synchronized Oscillating Jet Generation

NASA Technical Reports Server (NTRS)

Koklu, Mehti (Inventor)

2016-01-01

A fluidic oscillator array includes a plurality of fluidic-oscillator main flow channels. Each main flow channel has an inlet and an outlet. Each main flow channel has first and second control ports disposed at opposing sides thereof, and has a first and a second feedback ports disposed at opposing sides thereof. The feedback ports are located downstream of the control ports with respect to a direction of a fluid flow through the main flow channel. The system also includes a first fluid accumulator in fluid communication with each first control port and each first feedback port, and a second fluid accumulator in fluid communication with each second control port and each second feedback port.

Influence of the boundary conditions on heat and mass transfer in spacer-filled channels

NASA Astrophysics Data System (ADS)

Ciofalo, M.; La Cerva, M. F.; Di Liberto, M.; Tamburini, A.

2017-11-01

The purpose of this study is to discuss some problems which arise in heat or mass transfer in complex channels, with special reference to the spacer-filled channels adopted in membrane processes. Among the issues addressed are the consistent definition of local and mean heat or mass transfer coefficients; the influence of the wall boundary conditions; the influence of one-side versus two-side heat/mass transfer. Most of the results discussed were obtained by finite volume CFD simulations concerning heat transfer in Membrane Distillation or mass transfer in Electrodialysis and Reverse Electrodialysis, but many of the conclusions apply also to different processes involving geometrically complex channels

Computational analysis of sedimentation of two particles in a narrow channel

NASA Astrophysics Data System (ADS)

Aidun, Cyrus K.; Ding, Ejiang

1998-11-01

The motion and interaction of two spherical bodies of diameter d in a narrow channel (width 4d) is simulated by Lattice-Boltzmann method at Reynolds numbers between 0 and 10. The initial positions of the particles are midway between the centerline of the channel and the side wall while one particle is 2d above the other. At low Reynolds numbers, the particles oscillate around the centerline of the channel while they approach each other, and eventually settle in contact. At higher Reynolds numbers, the trailing particle approaches the leading one; jointly, the particles enter into a damping oscillation without contacting each other. This motion has been described as drafting, kissing and tumbling (Hu, Joseph, and Crochet, Theoret. Comput. Fluid Dyn. 3 1992; Feng, Hu, and Joseph, J. Fluid Mech. 261 1994). In the phase space, constructed by the distances between each particle and the side wall, the attractor is a fixed point, representing a steady state. At even higher Reynolds number the dynamics changes into a stable limit cycle. The amplitude of the limit cycle increases as the Reynolds number increases in value. As Reynolds number increases further the motion becomes more complex. The trajectory in the phase space suggests the existence of a strange attractor. The dynamics of two particle sedimentation at this range of Reynolds number will be presented.

Rapid prototyping of microfluidic systems using a PDMS/polymer tape composite.

PubMed

Kim, Jungkyu; Surapaneni, Rajesh; Gale, Bruce K

2009-05-07

Rapid prototyping of microfluidic systems using a combination of double-sided tape and PDMS (polydimethylsiloxane) is introduced. PDMS is typically difficult to bond using adhesive tapes due to its hydrophobic nature and low surface energy. For this reason, PDMS is not compatible with the xurography method, which uses a knife plotter and various adhesive coated polymer tapes. To solve these problems, a PDMS/tape composite was developed and demonstrated in microfluidic applications. The PDMS/tape composite was created by spinning it to make a thin layer of PDMS over double-sided tape. Then the PDMS/tape composite was patterned to create channels using xurography, and bonded to a PDMS slab. After removing the backing paper from the tape, a complete microfluidic system could be created by placing the construct onto nearly any substrate; including glass, plastic or metal-coated glass/silicon substrates. The bond strength was shown to be sufficient for the pressures that occur in typical microfluidic channels used for chemical or biological analysis. This method was demonstrated in three applications: standard microfluidic channels and reactors, a microfluidic system with an integrated membrane, and an electrochemical biosensor. The PDMS/tape composite rapid prototyping technique provides a fast and cost effective fabrication method and can provide easy integration of microfluidic channels with sensors and other components without the need for a cleanroom facility.

Patterns of fish assemblage structure and habitat use among main- and side-channel environments in the lower Kootenai River, Idaho

USGS Publications Warehouse

Watkins, Carson J.; Stevens, Bryan S.; Quist, Michael C.; Shepard, Bradley B.; Ireland, Susan C.

2015-01-01

The lower Kootenai River, Idaho, was sampled during the summers of 2012 and 2013 to evaluate its fish assemblage structure at seven sites within main- and side-channel habitats where large-scale habitat rehabilitation was undertaken. Understanding the current patterns of fish assemblage structure and their relationships with habitat is important for evaluating the effects of past and future rehabilitation projects on the river. Species-specific habitat associations were modeled, and the variables that best explained the occurrence and relative abundance of fish were identified in order to guide future habitat rehabilitation so that it benefits native species. The results indicated that the side-channel habitats supported higher species richness than the main-channel habitats and that nonnative fishes were closely associated with newly rehabilitated habitats. This research provides valuable insight on the current fish assemblages in the Kootenai River and the assemblage-level responses that may occur as a result of future rehabilitation activities.

Apparatus for the compact cooling of modules

DOEpatents

Iyengar, Madhusudan K.; Parida, Pritish R.

2015-07-07

An apparatus for the compact cooling of modules. The apparatus includes a clip, a first cover plate coupled to a first side of the clip, a second cover plate coupled to a second side of the clip opposite to the first side of the clip, a first frame thermally coupled to the first cover plate, and a second frame thermally coupled to the second cover plate. Each of the first frame and the second frame may include a plurality of channels for passing coolant through the first frame and the second frame, respectively. Additionally, the apparatus may further include a filler for directing coolant through the plurality of channels, and for blocking coolant from flowing along the first side of the clip and the second side of the clip.

Interaction of the BKCa channel gating ring with dendrotoxins

PubMed Central

Takacs, Zoltan; Imredy, John P; Bingham, Jon-Paul; Zhorov, Boris S; Moczydlowski, Edward G

2014-01-01

Two classes of small homologous basic proteins, mamba snake dendrotoxins (DTX) and bovine pancreatic trypsin inhibitor (BPTI), block the large conductance Ca2+-activated K+ channel (BKCa, KCa1.1) by production of discrete subconductance events when added to the intracellular side of the membrane. This toxin-channel interaction is unlikely to be pharmacologically relevant to the action of mamba venom, but as a fortuitous ligand-protein interaction, it has certain biophysical implications for the mechanism of BKCa channel gating. In this work we examined the subconductance behavior of 9 natural dendrotoxin homologs and 6 charge neutralization mutants of δ-dendrotoxin in the context of current structural information on the intracellular gating ring domain of the BKCa channel. Calculation of an electrostatic surface map of the BKCa gating ring based on the Poisson-Boltzmann equation reveals a predominantly electronegative surface due to an abundance of solvent-accessible side chains of negatively charged amino acids. Available structure-activity information suggests that cationic DTX/BPTI molecules bind by electrostatic attraction to site(s) on the gating ring located in or near the cytoplasmic side portals where the inactivation ball peptide of the β2 subunit enters to block the channel. Such an interaction may decrease the apparent unitary conductance by altering the dynamic balance of open versus closed states of BKCa channel activation gating. PMID:25483585

Methow River Studies, Washington: abundance estimates from Beaver Creek and the Chewuch River screw trap, methodology testing in the Whitefish Island side channel, and survival and detection estimates from hatchery fish releases, 2013

USGS Publications Warehouse

Martens, Kyle D.; Fish, Teresa M.; Watson, Grace A.; Connolly, Patrick J.

2014-01-01

Salmon and steelhead populations have been severely depleted in the Columbia River from factors such as the presence of tributary dams, unscreened irrigation diversions, and habitat degradation from logging, mining, grazing, and others (Raymond, 1988). The U.S. Geological Survey (USGS) has been funded by the Bureau of Reclamation (Reclamation) to provide evaluation of on-going Reclamation funded efforts to recover Endangered Species Act (ESA) listed anadromous salmonid populations in the Methow River watershed, a watershed of the Columbia River in the Upper Columbia River Basin, in north-central Washington State (fig. 1). This monitoring and evaluation program was funded to document Reclamation’s effort to partially fulfill the 2008 Federal Columbia River Power System Biological Opinion (BiOp) (National Oceanographic and Atmospheric Administration, Fisheries Division 2003). This Biological Opinion includes Reasonable and Prudent Alternatives (RPA) to protect listed salmon and steelhead across their life cycle. Species of concern in the Methow River include Upper Columbia River (UCR) spring Chinook salmon (Oncorhynchus tshawytscha), UCR summer steelhead (O. mykiss), and bull trout (Salvelinus confluentus), which are all listed as threatened or endangered under the ESA. The work done by the USGS since 2004 has encompassed three phases of work. The first phase started in 2004 and continued through 2012. This first phase involved the evaluation of stream colonization and fish production in Beaver Creek following the modification of several water diversions (2000–2006) that were acting as barriers to upstream fish movement. Products to date from this work include: Ruttenburg (2007), Connolly and others (2008), Martens and Connolly (2008), Connolly (2010), Connolly and others (2010), Martens and Connolly (2010), Benjamin and others (2012), Romine and others (2013a), Weigel and others (2013a, 2013b, 2013c), and Martens and others (2014). The second phase, initiated in 2008, focuses on the evaluation of the M2 reach (rkm 66– 80) of the mainstem Methow River prior to restoration actions planned by Reclamation and Yakama Nation. The M2 study was designed to help understand the inter-relationships between stream habitat and the life history of various fish species to explain potential success or limitations in response to restoration actions. To help document changes derived by restoration, two reference reaches (Upper Methow between rkm 85 and 90, and Chewuch River between rkm 4 and 11) were identified based on relative lack of disturbance, proximity to the restoration reach, and relative unconfined geomorphology. A control reach (Lower Methow between rkm 57 and 64, also referred to as “Silver Reach”) was 2 identified based on its similar disturbance as the reference reach, proximity to the restoration reach, and relatively unconfined geomorphology. Products to date include Barber and others (2011), Bellmore (2011), Tibbits and others (2012), Bellmore and others (2013), Benjamin and others (2013), Romine and others (2013b), Bellmore and other (2014), Martens and others (2014), and Martens and Connolly (2014). The third phase of work has been to help with the development and to provide data for modeling efforts. Most of the planned M2 reach restoration is focused on the creation or improvement of offchannel habitat, especially side channels. The pre-restoration portion of this study has been documented by Martens and Connolly (2014). Side channel restoration actions were initiated in 2012 (Whitefish Island side channel, also referred to as SC3; rkm 76) and are planned to continue over the next several years. The Whitefish Island side channel was modified to maintain hydrological connection with the mainstem throughout the year. In addition, several log structures were installed and pools were deepened to create fish habitat. Prior to restoration, this side channel would lose hydrological connection with the mainstem Methow River, leaving one large pool near the bottom of the side channel and several shallow isolated pools that may or may not go dry. In seasonally connected side channels, juvenile salmonid survival in pools less than 100 cm average depth was lower than in pools greater than 100 cm average depth (Martens and Connolly, 2014). In this report, we document our field work and analysis completed in 2013. During 2013, USGS sampling efforts were focused on resampling of three reaches in Beaver Creek, testing methodology in the Whitefish Island side channel, conducting hatchery survival estimates, and operating a screw trap on the Chewuch River (funded by Yakama Nation; fig. 1). The Beaver Creek sampling effort was a revisit of three index sites sampled continuously from 2004 to 2007 to look at the fish response to barrier removal. Methodology testing in Whitefish Island side channel was done to determine the best method for evaluating fish populations after restoration efforts in side channels (previous sampling methods were determined to be ineffective after pools were deepened). Hatchery survival estimates were completed to monitor fish survival in the Methow and Columbia Rivers, while the screw trap was operated to estimate migrating fish populations in the Chewuch River and track passive integrated transponder (PIT)-tagged fish. In addition, we maintained a network of PIT-tag interrogation systems (PTIS), assisted Reclamation with fish removal events associated with stream restoration (two people for 9 days; 14 percent of summer field season), and conducted a stream metabolism study designed to help parameterize and calibrate the stream productivity model (Bellmore and others, 2014) with model validation.

Diffusion of antimicrobials in multispecies biofilms evaluated in a new biofilm model.

PubMed

van der Waal, S V; de Almeida, J; Krom, B P; de Soet, J J; Crielaard, W

2017-04-01

To describe the application of a newly-developed in vitro model in which the diffusion of antimicrobials in oral biofilms can be studied. In a flow chamber consisting of three parallel feeding channels connected with each other by eight perpendicular side channels, multispecies biofilms were grown from saliva of a single donor for 48 h. The dimensions of the side channels were 100 μm × 100 μm × 5130 μm (H × W × L). When one or more side channels were filled with biofilm, the biofilms were stained with fluorescent stains. Then, one side-channel biofilm was selected and treated with phosphate buffered saline, 2% sodium hypochlorite (NaOCl), 17% ethylenediaminetetra-acetic acid (EDTA) or modified salt solution (MSS). Diffusion of the irrigants was observed by acquiring fluorescence images at 10× objective every 15 s for 30 min. It was possible to culture biofilms in the narrow (100 μm) channels. The biofilms varied in phenotype. In this model, no diffusion of NaOCl into the biofilms was seen after its application. Seventeen-percentage EDTA only diffused into the biofilm up to 200 μm in 30 min. MSS did diffuse in the biofilm over a distance of 450 μm within 2 min after a single application. This new model enables the investigation of the diffusion of antimicrobials in biofilms. Other applications to improve our understanding of the characteristics of biofilms are now possible. © 2016 International Endodontic Journal. Published by John Wiley & Sons Ltd.

An electrostatic mechanism for Ca2+-mediated regulation of gap junction channels

PubMed Central

Bennett, Brad C.; Purdy, Michael D.; Baker, Kent A.; Acharya, Chayan; McIntire, William E.; Stevens, Raymond C.; Zhang, Qinghai; Harris, Andrew L.; Abagyan, Ruben; Yeager, Mark

2016-01-01

Gap junction channels mediate intercellular signalling that is crucial in tissue development, homeostasis and pathologic states such as cardiac arrhythmias, cancer and trauma. To explore the mechanism by which Ca2+ blocks intercellular communication during tissue injury, we determined the X-ray crystal structures of the human Cx26 gap junction channel with and without bound Ca2+. The two structures were nearly identical, ruling out both a large-scale structural change and a local steric constriction of the pore. Ca2+ coordination sites reside at the interfaces between adjacent subunits, near the entrance to the extracellular gap, where local, side chain conformational rearrangements enable Ca2+chelation. Computational analysis revealed that Ca2+-binding generates a positive electrostatic barrier that substantially inhibits permeation of cations such as K+ into the pore. Our results provide structural evidence for a unique mechanism of channel regulation: ionic conduction block via an electrostatic barrier rather than steric occlusion of the channel pore. PMID:26753910

Performance Improvement of Power Analysis Attacks on AES with Encryption-Related Signals

NASA Astrophysics Data System (ADS)

Lee, You-Seok; Lee, Young-Jun; Han, Dong-Guk; Kim, Ho-Won; Kim, Hyoung-Nam

A power analysis attack is a well-known side-channel attack but the efficiency of the attack is frequently degraded by the existence of power components, irrelative to the encryption included in signals used for the attack. To enhance the performance of the power analysis attack, we propose a preprocessing method based on extracting encryption-related parts from the measured power signals. Experimental results show that the attacks with the preprocessed signals detect correct keys with much fewer signals, compared to the conventional power analysis attacks.

Abundance of Ohio shrimp (Macrobrachium ohione) and Glass shrimp (Palaemonetes kadiakensis) in the unimpounded Upper Mississippi River

USGS Publications Warehouse

Barko, V.A.; Hrabik, R.A.

2004-01-01

Large rivers of the United States have been altered by construction and maintenance of navigation channels, which has resulted in habitat loss and degradation. Using 7 y of Long Term Resource Monitoring Program data collected from the unimpounded upper Mississippi River, we investigated Ohio and Glass Shrimp abundance collected from four physical habitats of the unimpounded upper Mississippi River: main channel border, main channel border with wing dike, open side channel and closed side channel. Our objective was to assess associations between Ohio and Glass Shrimp abundance, environmental measurements and the four habitats to better understand the ecology of these species in a channelized river system. Ohio Shrimp were most abundant in the open side channels, while Glass Shrimp were most abundant in the main channel border wing dike habitat. Thirty-two percent of the variance in Glass Shrimp abundance was explained by year 1995, year 1998, water temperature, depth of gear deployment, Secchi disk transparency and river elevation. Approximately 8% of variation in Ohio Shrimp abundance was explained by Secchi disk transparency. Catch-per-unit-effort (CPUE) was greatest in 1998 for Glass Shrimp but lowest in 1997. Conversely, CPUE was greatest in 1996 for Ohio Shrimp and lowest in 2000. Both species exhibited inter-annual variability in CPUE. Long-term impacts of river modifications on aquatic invertebrates have not been well documented in many large, river systems and warrants further study. The findings from this study provide ecological information on Glass and Ohio Shrimp in a channelized river system.

Multi-channel orbicularis oculi stimulation to restore eye-blink function in facial paralysis.

PubMed

Somia, N N; Zonnevijlle, E D; Stremel, R W; Maldonado, C; Gossman, M D; Barker, J H

2001-01-01

Facial paralysis due to facial nerve injury results in the loss of function of the muscles of the hemiface. The most serious complication in extreme cases is the loss of vision. In this study, we compared the effectiveness of single- and multiple-channel electrical stimulation to restore a complete and cosmetically acceptable eye blink. We established bilateral orbicularis oculi muscle (OOM) paralysis in eight dogs; the OOM of one side was directly stimulated using single-channel electrical stimulation and the opposite side was stimulated using multi-channel electrical stimulation. The changes in the palpebral fissure and complete palpebral closure were measured. The difference in current intensities between the multi-channel and single-channel simulation groups was significant, while only multi-channel stimulation produced complete eyelid closure. The latest electronic stimulation circuitry with high-quality implantable electrodes will make it possible to regulate precisely OOM contractions and thus generate complete and cosmetically acceptable eye-blink motion in patients with facial paralysis. Copyright 2001 Wiley-Liss, Inc.

Side-chain conformation of the M2 transmembrane peptide proton channel of influenza a virus from 19F solid-state NMR.

PubMed

Luo, Wenbin; Mani, Rajeswari; Hong, Mei

2007-09-13

The M2 transmembrane peptide (M2TMP) of the influenza A virus forms a tetrameric helical bundle that acts as a proton-selective channel important in the viral life cycle. The side-chain conformation of the peptide is largely unknown and is important for elucidating the proton-conducting mechanism and the channel stability. Using a 19F spin diffusion NMR technique called CODEX, we have measured the oligomeric states and interhelical side chain-side chain 19F-19F distances at several residues using singly fluorinated M2TMP bound to DMPC bilayers. 19F CODEX data at a key residue of the proton channel, Trp41, confirm the tetrameric state of the peptide and yield a nearest-neighbor interhelical distance of approximately 11 A under both neutral and acidic pH. Since the helix orientation is precisely known from previous 15N NMR experiments and the backbone channel diameter has a narrow allowed range, this 19F distance constrains the Trp41 side-chain conformation to t90 (chi1 approximately 180 degrees , chi2 approximately 90 degrees ). This Trp41 rotamer, combined with a previously measured 15N-13C distance between His37 and Trp411, suggests that the His37 rotamer is t-160. The implication of the proposed (His37, Trp41) rotamers to the gating mechanism of the M2 proton channel is discussed. Binding of the antiviral drug amantadine to the peptide does not affect the F-F distance at Trp41. Interhelical 19F-19F distances are also measured at residues 27 and 38, each mutated to 4-19F-Phe. For V27F-M2TMP, the 19F-19F distances suggest a mixture of dimers and tetramers, whereas the L38F-M2TMP data indicate two tetramers of different sizes, suggesting side chain conformational heterogeneity at this lipid-facing residue. This work shows that 19F spin diffusion NMR is a valuable tool for determining long-range intermolecular distances that shed light on the mechanism of action and conformational heterogeneity of membrane protein oligomers.

Redistribution of Cav2.1 channels and calcium ions in nerve terminals following end-to-side neurorrhaphy: ionic imaging analysis by TOF-SIMS.

PubMed

Liu, Chiung-Hui; Chang, Hung-Ming; Tseng, To-Jung; Lan, Chyn-Tair; Chen, Li-You; Youn, Su-Chung; Lee, Jian-Jr; Mai, Fu-Der; Chou, Jui-Feng; Liao, Wen-Chieh

2016-11-01

The P/Q-type voltage-dependent calcium channel (Cav2.1) in the presynaptic membranes of motor nerve terminals plays an important role in regulating Ca 2+ transport, resulting in transmitter release within the nervous system. The recovery of Ca 2+ -dependent signal transduction on motor end plates (MEPs) and innervated muscle may directly reflect nerve regeneration following peripheral nerve injury. Although the functional significance of calcium channels and the levels of Ca 2+ signalling in nerve regeneration are well documented, little is known about calcium channel expression and its relation with the dynamic Ca 2+ ion distribution at regenerating MEPs. In the present study, end-to-side neurorrhaphy (ESN) was performed as an in vivo model of peripheral nerve injury. The distribution of Ca 2+ at regenerating MEPs following ESN was first detected by time-of-flight secondary ion mass spectrometry, and the specific localization and expression of Cav2.1 channels were examined by confocal microscopy and western blotting. Compared with other fundamental ions, such as Na + and K + , dramatic changes in the Ca 2+ distribution were detected along with the progression of MEP regeneration. The re-establishment of Ca 2+ distribution and intensity were correlated with the functional recovery of muscle in ESN rats. Furthermore, the re-clustering of Cav2.1 channels after ESN at the nerve terminals corresponded with changes in the Ca 2+ distribution. These results indicated that renewal of the Cav2.1 distribution within the presynaptic nerve terminals may be necessary for initiating a proper Ca 2+ influx and shortening the latency of muscle contraction during nerve regeneration.

Science to support adaptive habitat management: Overton Bottoms North Unit, Big Muddy National Fish and Wildlife Refuge, Missouri [Volumes 1-6

USGS Publications Warehouse

Jacobson, Robert B.

2006-01-01

Extensive efforts are underway along the Lower Missouri River to rehabilitate ecosystem functions in the channel and flood plain. Considerable uncertainty inevitably accompanies ecosystem restoration efforts, indicating the benefits of an adaptive management approach in which management actions are treated as experiments, and results provide information to feed back into the management process. The Overton Bottoms North Unit of the Big Muddy National Fish and Wildlife Refuge is a part of the Missouri River Fish and Wildlife Habitat Mitigation Project. The dominant management action at the Overton Bottoms North Unit has been excavation of a side-channel chute to increase hydrologic connectivity and to enhance shallow, slow current-velocity habitat. The side-channel chute also promises to increase hydrologic gradients, and may serve to alter patterns of wetland inundation and vegetation community growth in undesired ways. The U.S. Geological Survey's Central Region Integrated Studies Program (CRISP) undertook interdisciplinary research at the Overton Bottoms North Unit in 2003 to address key areas of scientific uncertainty that were highly relevant to ongoing adaptive management of the site, and to the design of similar rehabilitation projects on the Lower Missouri River. This volume presents chapters documenting the surficial geologic, topographic, surface-water, and ground-water framework of the Overton Bottoms North Unit. Retrospective analysis of vegetation community trends over the last 10 years is used to evaluate vegetation responses to reconnection of the Overton Bottoms North Unit to the river channel. Quasi-experimental analysis of cottonwood growth rate variation along hydrologic gradients is used to evaluate sensitivity of terrestrial vegetation to development of aquatic habitats. The integrated, landscape-specific understanding derived from these studies illustrates the value of scientific information in design and management of rehabilitation projects.

Charged Particle Periodicities in Saturn's Outer Magnetosphere

NASA Astrophysics Data System (ADS)

Carbary, J.; Mitchell, D.; Krimigis, S.; Krupp, N.

2006-12-01

The MIMI/LEMMS instrument on the Cassini spacecraft has measured energetic electrons in the energy range 20-300 keV within Saturn's magnetosphere. In the outer magnetosphere beyond about 20 RS, these electrons and their spectral index display strong variations with periods comparable to the 10.76 hour period measured by radio observations of Cassini. Inside about 20 RS, such electron variations may be present but are masked by satellite and ring effects. Electron periodicities are most easily recognized on the "night side" segments of the Cassini orbits, although they are also observed to some extent on the day side. For both day and night sides, a wavelet analysis of de-trended count rates in the 20-40 RS region reveals a mean period of 10.52 +/- 0.74 hrs for the six electron channels investigated. If constrained to the night side only, a wavelet analysis gives a mean period of 10.88 +/- 0.52 hours. These periods were obtained from several orbits of the Cassini spacecraft during the two-year period from SOI (July 2004) to the present (November 2006).

Extending Differential Fault Analysis to Dynamic S-Box Advanced Encryption Standard Implementations

DTIC Science & Technology

2014-09-18

entropy . At the same time, researchers strive to enhance AES and mitigate these growing threats. This paper researches the extension of existing...the algorithm or use side channels to reduce entropy , such as Differential Fault Analysis (DFA). At the same time, continuing research strives to...the state matrix. The S-box is an 8-bit 16x16 table built from an affine transformation on multiplicative inverses which guarantees full permutation (S

A novel cross-species inhibitor to study the function of CatSper Ca2+ channels in sperm.

PubMed

Rennhack, Andreas; Schiffer, Christian; Brenker, Christoph; Fridman, Dmitry; Nitao, Elis T; Cheng, Yi-Min; Tamburrino, Lara; Balbach, Melanie; Stölting, Gabriel; Berger, Thomas K; Kierzek, Michelina; Alvarez, Luis; Wachten, Dagmar; Zeng, Xu-Hui; Baldi, Elisabetta; Publicover, Stephen; Kaupp, U Benjamin; Strünker, Timo

2018-05-03

Sperm from many species share the sperm-specific Ca 2+ channel CatSper (cation channel of sperm) that controls the intracellular Ca 2+ concentration and, thereby, the swimming behaviour. A growing body of evidence suggests that the mechanisms controlling CatSper activity and the role of the channel during fertilization differ among species. However, a lack of suitable pharmacological tools has hampered the elucidation of the function of CatSper. Known CatSper inhibitors exhibit considerable side effects and inhibit also Slo3, the K + channel in mammalian sperm. The drug RU1968 was reported to suppress Ca 2+ signaling in human sperm by an unknown mechanism. We resynthesized the drug and revisited its mechanism of action in sperm form humans, mice, and sea urchins. We show by Ca 2+ fluorimetry, single-cell Ca 2+ imaging, electrophysiology, opto-chemistry, and motility analysis that RU1968 inhibits CatSper in sperm from invertebrates and mammals. The drug lacks toxic side effects in human sperm, does not affect mouse Slo3, and inhibits human Slo3 with about 15-fold lower potency than CatSper. Moreover, in human sperm, the inhibitor mimics CatSper dysfunction and suppresses motility responses evoked by progesterone, an oviductal steroid that activates CatSper. Finally, we show that the drug abolishes CatSper-mediated chemotactic navigation in sea urchin sperm. We propose RU1968 as a novel tool to elucidate the function of CatSper in sperm across species. This article is protected by copyright. All rights reserved.

Impact of scaling voltage and size on the performance of Side-contacted Field Effect Diode

NASA Astrophysics Data System (ADS)

Touchaei, Behnam Jafari; Manavizadeh, Negin

2018-05-01

Side-contacted Fild Effect Diode (S-FED), with low leakage current and high Ion/Ioff ratio, has been recently introduced to suppress short channel effects in nanoscale regime. The voltage and size scalability of S-FEDs and effects on the power consumption, propagation delay time, and power delay product have been studied in this article. The most attractive properties are related to channel length to channel thickness ratio in the S-FED which reduces in comparison with MOSFET significantly, while gates control over the channel improve and the off-state current reduces dramatically. This promising advantage is not only capable to improve important S-FED's characteristics such as subthreshold slope but also eliminate Latch-up and floating body effect.

Side-information-dependent correlation channel estimation in hash-based distributed video coding.

PubMed

Deligiannis, Nikos; Barbarien, Joeri; Jacobs, Marc; Munteanu, Adrian; Skodras, Athanassios; Schelkens, Peter

2012-04-01

In the context of low-cost video encoding, distributed video coding (DVC) has recently emerged as a potential candidate for uplink-oriented applications. This paper builds on a concept of correlation channel (CC) modeling, which expresses the correlation noise as being statistically dependent on the side information (SI). Compared with classical side-information-independent (SII) noise modeling adopted in current DVC solutions, it is theoretically proven that side-information-dependent (SID) modeling improves the Wyner-Ziv coding performance. Anchored in this finding, this paper proposes a novel algorithm for online estimation of the SID CC parameters based on already decoded information. The proposed algorithm enables bit-plane-by-bit-plane successive refinement of the channel estimation leading to progressively improved accuracy. Additionally, the proposed algorithm is included in a novel DVC architecture that employs a competitive hash-based motion estimation technique to generate high-quality SI at the decoder. Experimental results corroborate our theoretical gains and validate the accuracy of the channel estimation algorithm. The performance assessment of the proposed architecture shows remarkable and consistent coding gains over a germane group of state-of-the-art distributed and standard video codecs, even under strenuous conditions, i.e., large groups of pictures and highly irregular motion content.

Non-uniform overland flow-infiltration model for roadside swales

NASA Astrophysics Data System (ADS)

García-Serrana, María; Gulliver, John S.; Nieber, John L.

2017-09-01

There is a need to quantify the hydrologic performance of vegetated roadside swales (drainage ditches) as stormwater control measures (SCMs). To quantify their infiltration performance in both the side slope and the channel of the swale, a model has been developed for coupling a Green-Ampt-Mein-Larson (GAML) infiltration submodel with kinematic wave submodels for both overland flow down the side slope and open channel flow for flow in the ditch. The coupled GAML submodel and overland flow submodel has been validated using data collected in twelve simulated runoff tests in three different highways located in the Minneapolis-St. Paul metropolitan area, MN. The percentage of the total water infiltrated into the side slope is considerably greater than into the channel. Thus, the side slope of a roadside swale is the main component contributing to the loss of runoff by infiltration and the channel primarily conveys the water that runs off the side slope, for the typical design found in highways. Finally, as demonstrated in field observations and the model, the fraction of the runoff/rainfall infiltrated (Vi∗) into the roadside swale appears to increase with a dimensionless saturated hydraulic conductivity (Ks∗), which is a function of the saturated hydraulic conductivity, rainfall intensity, and dimensions of the swale and contributing road surface. For design purposes, the relationship between Vi∗ and Ks∗ can provide a rough estimate of the fraction of runoff/rainfall infiltrated with the few essential parameters that appear to dominate the results.

Sequential cooling insert for turbine stator vane

DOEpatents

Jones, Russel B

2017-04-04

A sequential flow cooling insert for a turbine stator vane of a small gas turbine engine, where the impingement cooling insert is formed as a single piece from a metal additive manufacturing process such as 3D metal printing, and where the insert includes a plurality of rows of radial extending impingement cooling air holes alternating with rows of radial extending return air holes on a pressure side wall, and where the insert includes a plurality of rows of chordwise extending second impingement cooling air holes on a suction side wall. The insert includes alternating rows of radial extending cooling air supply channels and return air channels that form a series of impingement cooling on the pressure side followed by the suction side of the insert.

Arginine side chain interactions and the role of arginine as a gating charge carrier in voltage sensitive ion channels

PubMed Central

Armstrong, Craig T.; Mason, Philip E.; Anderson, J. L. Ross; Dempsey, Christopher E.

2016-01-01

Gating charges in voltage-sensing domains (VSD) of voltage-sensitive ion channels and enzymes are carried on arginine side chains rather than lysine. This arginine preference may result from the unique hydration properties of the side chain guanidinium group which facilitates its movement through a hydrophobic plug that seals the center of the VSD, as suggested by molecular dynamics simulations. To test for side chain interactions implicit in this model we inspected interactions of the side chains of arginine and lysine with each of the 19 non-glycine amino acids in proteins in the protein data bank. The arginine guanidinium interacts with non-polar aromatic and aliphatic side chains above and below the guanidinium plane while hydrogen bonding with polar side chains is restricted to in-plane positions. In contrast, non-polar side chains interact largely with the aliphatic part of the lysine side chain. The hydration properties of arginine and lysine are strongly reflected in their respective interactions with non-polar and polar side chains as observed in protein structures and in molecular dynamics simulations, and likely underlie the preference for arginine as a mobile charge carrier in VSD. PMID:26899474

Arginine side chain interactions and the role of arginine as a gating charge carrier in voltage sensitive ion channels

NASA Astrophysics Data System (ADS)

Armstrong, Craig T.; Mason, Philip E.; Anderson, J. L. Ross; Dempsey, Christopher E.

2016-02-01

Gating charges in voltage-sensing domains (VSD) of voltage-sensitive ion channels and enzymes are carried on arginine side chains rather than lysine. This arginine preference may result from the unique hydration properties of the side chain guanidinium group which facilitates its movement through a hydrophobic plug that seals the center of the VSD, as suggested by molecular dynamics simulations. To test for side chain interactions implicit in this model we inspected interactions of the side chains of arginine and lysine with each of the 19 non-glycine amino acids in proteins in the protein data bank. The arginine guanidinium interacts with non-polar aromatic and aliphatic side chains above and below the guanidinium plane while hydrogen bonding with polar side chains is restricted to in-plane positions. In contrast, non-polar side chains interact largely with the aliphatic part of the lysine side chain. The hydration properties of arginine and lysine are strongly reflected in their respective interactions with non-polar and polar side chains as observed in protein structures and in molecular dynamics simulations, and likely underlie the preference for arginine as a mobile charge carrier in VSD.

Basolateral K channels in an insect epithelium. Channel density, conductance, and block by barium

PubMed Central

Hanrahan, JW; Wills, NK; Phillips, JE; Lewis, SA

1986-01-01

K channels in the basolateral membrane of insect hindgut were studied using current fluctuation analysis and microelectrodes. Locust recta were mounted in Ussing-type chambers containing Cl-free saline and cyclic AMP (cAMP). A transepithelial K current was induced by raising serosal [K] under short-circuit conditions. Adding Ba to the mucosal (luminal) side under these conditions had no effect; however, serosal Ba reversibly inhibited the short-circuit current (Isc), increased transepithelial resistance (Rt), and added a Lorentzian component to power density spectra of the Isc. A nonlinear relationship between corner frequency and serosal [Ba] was observed, which suggests that the rate constant for Ba association with basolateral channels increased as [Ba] was elevated. Microelectrode experiments revealed that the basolateral membrane hyperpolarized when Ba was added: this change in membrane potential could explain the nonlinearity of the 2 pi fc vs. [Ba] relationship if external Ba sensed about three-quarters of the basolateral membrane field. Conventional microelectrodes were used to determine the correspondence between transepithelially measured current noise and basolateral membrane conductance fluctuations, and ion-sensitive microelectrodes were used to measure intracellular K activity (acK). From the relationship between the net electrochemical potential for K across the basolateral membrane and the single channel current calculated from noise analysis, we estimate that the conductance of basolateral K channels is approximately 60 pS, and that there are approximately 180 million channels per square centimeter of tissue area. PMID:2420918

Recent benthic foraminifera assemblages from mangrove swamp and channels of Abu Dhabi (UAE)

NASA Astrophysics Data System (ADS)

Fiorini, Flavia; Lokier, Stephen W.; Odeh, Weaam A. S. Al; Paul, Andreas; Song, Jianfeng; Freeman, Mark; Michel, Françoise

2017-04-01

Zonation of Recent mangrove environments can be defined using benthic foraminifera, however, little is known about foraminifera from mangrove environments of the Persian/Arabian Gulf. The objective of this study is to produce a detailed micropaleontological and sedimentological analysis to identify foraminiferal associations from mangrove swamps and channels located on the eastern side of Abu Dhabi Island (UAE). Detailed sediment sampling collection in mangal environments of Eastern Abu Dhabi was carried out to assess the distribution of benthic foraminifera in different sedimentary facies in the mangal and in the surrounding natural environments of the upper and lower intertidal area (mud flats and channels). A 100 m transect across a natural channel in a mangal on the eastern side of Abu Dhabi Island was sampled in detail for sedimentological and foraminiferal analysis. Forty-seven samples were collected at 2 meter intervals along the transect in a number of different sedimentary facies including; fine sediment in areas exposed during low tide and close to mangrove trees (Avicennia marina), fine sediment rich in leaf material, coarse sediment in channels, and coarse sediments with a shell lag. At each sampling location environmental parameters were recorded, including water depth, salinity, temperature and pH. Samples collected for foraminiferal analysis were stained in rose Bengal in order to identify living specimens. Samples collected on the mud flat at the margin of the channel show a living foraminiferal assemblage characterised by abundant foraminifera belonging to the genera Ammonia, Elphidium, Cribroelphidium, Triloculina, Quinqueloculina, Sigmoilinita, Spiroloculina, Peneroplis and Spirolina. Samples collected in the lower (wet) intertidal area close to Avicennia marina roots, presented a low-diversity assemblage mostly comprising small-sized opportunistic foraminifera of the genera Ammonia and Cribroelphidium along with rare Triloculina and Quinqueloculina. Samples from the upper intertidal areas (often dry) close to Avicennia marina roots and leaf material, produced an assemblage exclusively composed of small-sized opportunistic Ammonia and Cribroelphidium, together with abundant specimensof agglutinated foraminifera belonging to the genera Trochammina. The samples collected in the higher energy settings (channels) were rich in foraminiferal tests, rare living forms were found in the coarser grained facies. The more abundant genera of foraminifera in these facies were miliolids belonging to the genera Triloculina, Quinqueloculina, Sigmoilina and epiphytic larger benthic foraminifera belonging to the genera Peneroplis, Spirolina and Sorites. The distribution of Recent benthic foraminifera from the mangrove environments of the Abu Dhabi region present a powerful tool for constructing a zonation of marine coastline environments and can be employed as a modern analogue for interpreting the depositional environment of ancient coastline sediments.

Anatomy and growth pattern of Amazon deep-sea fan as revealed by long-range side-scan sonar (GLORIA) and high-resolution seismic studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Damuth, J.E.; Flood, R.D.; Kowsmann, R.O.

1988-08-01

Imaging of the Amazon deep-sea fan with long-range side-scan sonar (GLORIA) has, for the first time, revealed the anatomy, trends, and growth pattern of distributary channels on this fan. Only one channel-levee system was active at any given time and extended from the Amazon Submarine Canyon downslope onto the lower fan (> 4,200 m). Formation of new channel-levee systems occurred when a currently active channel-levee system was cut off and abandoned through avulsion, and a new channel-levee system was established nearby. Through time, successive channel-levee formation and abandonment built two broad levee complexes consisting of groups of overlapping, coalescing segmentsmore » of channel-levee systems across the present fan surface. These, plus older, now buried levee complexes, indicate that fan growth is radially outward and downslope through development of successive levee complexes. The most striking characteristic of the distributary channels is their intricate, often recurving, meanders with sinuosities of up to 2.5. Cutoffs and abandoned meander loops indicate that the channels migrate laterally through time. Channel bifurcation results predominantly from avulsion when flows breach a channel levee, thereby abandoning the present channel and establishing a new channel-levee segment nearby. No clear evidence of channel branching (i.e., division of a single channel into two active segments) or braiding was observed. 22 figs.« less

Continuous separation of microparticles in a microfluidic channel via the elasto-inertial effect of non-Newtonian fluid.

PubMed

Nam, Jeonghun; Lim, Hyunjung; Kim, Dookon; Jung, Hyunwook; Shin, Sehyun

2012-04-07

Pure separation and sorting of microparticles from complex fluids are essential for biochemical analyses and clinical diagnostics. However, conventional techniques require highly complex and expensive labeling processes for high purity separation. In this study, we present a simple and label-free method for separating microparticles with high purity using the elasto-inertial characteristic of a non-Newtonian fluid in microchannel flow. At the inlet, particle-containing sample flow was pushed toward the side walls by introducing sheath fluid from the center inlet. Particles of 1 μm and 5 μm in diameter, which were suspended in viscoelastic fluid, were successfully separated in the outlet channels: larger particles were notably focused on the centerline of the channel at the outlet, while smaller particles continued flowing along the side walls with minimal lateral migration towards the centerline. The same technique was further applied to separate platelets from diluted whole blood. Through cytometric analysis, we obtained a purity of collected platelets of close to 99.9%. Conclusively, our microparticle separation technique using elasto-inertial forces in non-Newtonian fluid is an effective method for separating and collecting microparticles on the basis of size differences with high purity. This journal is © The Royal Society of Chemistry 2012

Multi-pass cooling for turbine airfoils

DOEpatents

Liang, George [Palm City, FL

2011-06-28

An airfoil for a turbine vane of a gas turbine engine. The airfoil includes an outer wall having pressure and suction sides, and a radially extending cooling cavity located between the pressure and suction sides. A plurality of partitions extend radially through the cooling cavity to define a plurality of interconnected cooling channels located at successive chordal locations through the cooling cavity. The cooling channels define a serpentine flow path extending in the chordal direction. Further, the cooling channels include a plurality of interconnected chambers and the chambers define a serpentine path extending in the radial direction within the serpentine path extending in the chordal direction.

Central tracker for BM@N experiment based on double side Si-microstrip detectors

NASA Astrophysics Data System (ADS)

Kovalev, Yu.; Kapishin, M.; Khabarov, S.; Shafronovskaia, A.; Tarasov, O.; Makankin, A.; Zamiatin, N.; Zubarev, E.

2017-07-01

Design of central tracker system based on Double-Sided Silicon Detectors (DSSD) for BM@N experiment is described. A coordinate plane with 10240 measuring channels, pitch adapter, reading electronics was developed. Each element was tested and assembled into a coordinate plane. The first tests of the plane with 106Ru source were carried out before installation for the BM@N experiment. The results of the study indicate that noisy channels and inefficient channels are less than 3%. In general, single clusters 87% (one group per module of consecutive strips) and 75% of clusters with a width equal to one strip.

Solar thermoelectric cooling using closed loop heat exchangers with macro channels

NASA Astrophysics Data System (ADS)

Atta, Raghied M.

2017-07-01

In this paper we describe the design, analysis and experimental study of an advanced coolant air conditioning system which cools or warms airflow using thermoelectric (TE) devices powered by solar cells. Both faces of the TE devices are directly connected to closed-loop highly efficient channels plates with macro scale channels and liquid-to-air heat exchangers. The hot side of the system consists of a pump that moves a coolant through the hot face of the TE modules, a radiator that drives heat away into the air, and a fan that transfer the heat over the radiator by forced convection. The cold side of the system consists also of a pump that moves coolant through the cold face of the TE modules, a radiator that drives cold away into the air, and a fan that blows cold air off the radiator. The system was integrated with solar panels, tested and its thermal performance was assessed. The experimental results verify the possibility of heating or cooling air using TE modules with a relatively high coefficient of performance (COP). The system was able to cool a closed space of 30 m3 by 14 °C below ambient within 90 min. The maximum COP of the whole system was 0.72 when the TE modules were running at 11.2 Å and 12 V. This improvement in the system COP over the air cooled heat sink is due to the improvement of the system heat exchange by means of channels plates.

Hot gas path component trailing edge having near wall cooling features

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lacy, Benjamin Paul; Kottilingam, Srikanth Chandrudu; Miranda, Carlos Miguel

A hot gas path component includes a substrate having an outer surface and an inner surface. The inner surface defines an interior space. The outer surface defines a pressure side surface and a suction side surface. The pressure and suction side surfaces are joined together at a leading edge and at a trailing edge. A first cooling passage is formed in the suction side surface of the substrate. It is coupled in flow communication to the interior space. A second cooling passage, separate from the first cooling passage, is formed in the pressure side surface. The second cooling passage ismore » coupled in flow communication to the interior space. A cover is disposed over at least a portion of the first and second cooling passages. The interior space channels a cooling fluid to the first and second cooling passages, which channel the cooling fluid therethrough to remove heat from the component.« less

Near-wall serpentine cooled turbine airfoil

DOEpatents

Lee, Ching-Pang

2013-09-17

A serpentine coolant flow path (54A-54G) formed by inner walls (50, 52) in a cavity (49) between pressure and suction side walls (22, 24) of a turbine airfoil (20A). A coolant flow (58) enters (56) an end of the airfoil, flows into a span-wise channel (54A), then flows forward (54B) over the inner surface of the pressure side wall, then turns behind the leading edge (26), and flows back along a forward part of the suction side wall, then follows a loop (54E) forward and back around an inner wall (52), then flows along an intermediate part of the suction side wall, then flows into an aft channel (54G) between the pressure and suction side walls, then exits the trailing edge (28). This provides cooling matched to the heating topography of the airfoil, minimizes differential thermal expansion, revives the coolant, and minimizes the flow volume needed.

6. VIEW OF DAM 83, SHOWING OUTLET CHANNEL FLOWING INTO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. VIEW OF DAM 83, SHOWING OUTLET CHANNEL FLOWING INTO POND A WITH DIVERSION GATES LONG EAST (LEFT) SIDE OF OUTLET CHANNEL, LOOKING SOUTH FROM DOWNSTREAM FACE OF THE DAM - Upper Souris National Wildlife Refuge, Dam 83, Souris River Basin, Foxholm, Surrey (England), ND

3D Seismic Stratigraphic Analysis of Gas Hydrate Bearing Turbidite Channel-Overbank System in Northern Gulf of Mexico

NASA Astrophysics Data System (ADS)

Santra, M.; Flemings, P. B.; Scott, E.; Meazell, K.; Petrou, E. G.

2017-12-01

We present a depositional model for a gas hydrate bearing deepwater channel-overbank system in Green Canyon area (around Block 955) in northern Gulf of Mexico. The gas-hydrate bearing reservoir was tested by three wells drilled in 2009 as part of the Gulf of Mexico Gas Hydrate Joint Industry Project (JIP). The same reservoir was sampled during the recent UT-GOM2-1 pressure-coring expedition. Analysis of a newly available wide-azimuth 3D seismic data shows two distinct stages of development of the channel system that significantly impacted the reservoir characteristics. The study area is located near the present-day Green Canyon reentrant, where a succession of Miocene to recent clastic sediments overlies an extensive salt diapir connected to the autochthonous level. The entire supra-salt sedimentary section is intersected by a system of large-scale normal faults formed as a result of salt movement. The channel system containing the gas hydrate reservoir has a well-defined basal surface, and is capped by a channel abandonment surface. Seismic analysis shows at least two distinct phases of channel development. In the first phase, levees undergo progressive gravitational collapse along series of normal faults that dip towards the channel axis. The normal faults on either side of channel axis are linked to a zone of compression located at the channel axis by a decollement surface at the base of the channel. The compression is recorded by bulging and/or thrusting at the channel center. This compressional bulge was eroded at the channel axis. During this phase, no axial channel deposits have been preserved. However, the position of the channel axis is indicated by a prominent linear ridge of fine-grained material that represents the remnant of the compressional bulge. Mapping of gravitational failure surfaces shows significant rotation and displacement of levee deposits along them. The second phase of development of the channel system is marked by the termination of gravitational failure and the preservation of both channel deposits and flanking levees. Both gravitational failure of channel-levee system and large-scale normal faulting impacted hydrate reservoir configuration. The large-scale fault system may have been the major pathway for hydrocarbon migration.

Low-complexity video encoding method for wireless image transmission in capsule endoscope.

PubMed

Takizawa, Kenichi; Hamaguchi, Kiyoshi

2010-01-01

This paper presents a low-complexity video encoding method applicable for wireless image transmission in capsule endoscopes. This encoding method is based on Wyner-Ziv theory, in which side information available at a transmitter is treated as side information at its receiver. Therefore complex processes in video encoding, such as estimation of the motion vector, are moved to the receiver side, which has a larger-capacity battery. As a result, the encoding process is only to decimate coded original data through channel coding. We provide a performance evaluation for a low-density parity check (LDPC) coding method in the AWGN channel.

Analysis of current and temperature data collected in the channel of Sardinia during the SALTO and MFSPP experiments

NASA Astrophysics Data System (ADS)

Rougier, G.; Millot, C.; Chretien, E.; Gervais, T.; Fuda, J.-L.; Sammari, C.

2003-04-01

The SALTO experiment was conducted from July 1999 to April 2000 to study the intermediate and deep circulation in the channel of Sardinia. Five moorings were aligned from southeast (M1) to northwest (M5) along one of the XBT lines monitored twice monthly during the MFSPP experiment. They were set about 10 miles apart over isobaths of 1000-2000 m, and they were equipped with 12 current meters at 500 and 900 m (M1, M5), 1000 and 1400 m (M2, M4), 500, 1000, 1400 and 1900 m (M3). The time series were first qualified according to the analysis of the tidal components, which have amplitudes ranging from a few mm/s to about 1 cm/s there (Albérola et al., 1995). Consistently with the current time series collected in 1993-1994 during the PRIMO-1 experiment (Bouzinac et al., 1999), the mean circulation on the Sardinian side (M4, M5) was a few cm/s alongslope from the Tyrrhenian subbasin. The mean circulation in the middle of the channel (M3), which was low and erratic close to the bottom in 1993-1994, was now significant (a few cm/s) and directed eastwards, roughly along the channel axis there. The mean circulation on the Tunisian side (M1, M2), which was never measured before, was low at M1 and significant (a few cm/s) at M2, but strangely south-southeastwards, i.e. perpendicular to the slope. The overall temperature distribution was consistent with that previously described by Bouzinac et al. (1999) and by Sammari et al. (1999). Since the sole significant circulation of water of Atlantic origin is eastwards along Tunisia, isotherms in a 200-300 m surface layer are sloping down southwards there. Since waters at intermediate and greater depths are relatively warm (resp. cool) on the Sardinian side (resp. Tunisian side), all isotherms below about 500 m are sloping up southwards. Mean differences of about 0.4 °C between one side of the channel and the other are permanently encountered at depths ranging from about 500 to 1500 m. However, the variability is much larger on the Tunisian side. None of the EOF analyses performed with either the original time series or the low-passed filtered ones allows defining an energetic mode significantly involving all time series; hence, there was no overall circulation at intermediate and greater depths. Mode 1 (about 30% of the total variance) involves M4 and M5 with similar phases at both moorings and rectilinear variations in the same direction; it corresponds to fluctuations of the flow from the Tyrrhenian subbasin along the Sardinian slope. Mode 2 (about 25%) involves M3 and M4 with specific phases at each mooring (different by less than about 40°) and marked changes in direction; it corresponds to fluctuations of the flow from the Algerian subbasin that sometimes concern the lower part of the Sardinian slope. Mode 4 (about 20%), which involves M2 alone, corresponds to fluctuations that are specific to the lower part of the Tunisian slope. Thanks are due to the crew of the R.V. Hannibal managed by INSTM. REFERENCES Albérola C., S. Rousseau, C. Millot, M. Astraldi, J. Font, J. Garcia-Lafuente, G.P. Gasparini, U. Send and A. Vangriesheim, 1995. Tidal currents in the interior of the Western Mediterranean Sea. Oceanol. Acta, 18, 2, 273-284. Bouzinac C., J. Font and C. Millot, 1999. Hydrology and currents observed in the channel of Sardinia during the PRIMO-1 experiment from November 1993 to October 1994. J. Mar. Systems, 20, 1-4, 333-355. Sammari C., C. Millot, I. Taupier-Letage, A. Stefani and M. Brahim, 1999. Hydrological characteristics in the Tunisia-Sicily-Sardinia area during spring 1995. Deep-Sea Res., I-46, 1671-1703.

NUCLEAR REACTOR FUEL ELEMENT ASSEMBLY

DOEpatents

Stengel, F.G.

1963-12-24

A method of fabricating nuclear reactor fuel element assemblies having a plurality of longitudinally extending flat fuel elements in spaced parallel relation to each other to form channels is presented. One side of a flat side plate is held contiguous to the ends of the elements and a welding means is passed along the other side of the platertransverse to the direction of the longitudinal extension of the elements. The setting and speed of travel of the welding means is set to cause penetration of the side plate with welds at bridge the gap in each channel between adjacent fuel elements with a weld-through bubble of predetermined size. The fabrication of a high strength, dependable fuel element is provided, and the reduction of distortion and high production costs are facilitated by this method. (AEC)

Heat transfer in a compact heat exchanger containing rectangular channels and using helium gas

NASA Technical Reports Server (NTRS)

Olson, D. A.

1991-01-01

Development of a National Aerospace Plane (NASP), which will fly at hypersonic speeds, require novel cooling techniques to manage the anticipated high heat fluxes on various components. A compact heat exchanger was constructed consisting of 12 parallel, rectangular channels in a flat piece of commercially pure nickel. The channel specimen was radiatively heated on the top side at heat fluxes of up to 77 W/sq cm, insulated on the back side, and cooled with helium gas flowing in the channels at 3.5 to 7.0 MPa and Reynolds numbers of 1400 to 28,000. The measured friction factor was lower than that of the accepted correlation for fully developed turbulent flow, although the uncertainty was high due to uncertainty in the channel height and a high ratio of dynamic pressure to pressure drop. The measured Nusselt number, when modified to account for differences in fluid properties between the wall and the cooling fluid, agreed with past correlations for fully developed turbulent flow in channels. Flow nonuniformity from channel-to-channel was as high as 12 pct above and 19 pct below the mean flow.

Cyclic joint remote state preparation in noisy environment

NASA Astrophysics Data System (ADS)

Zhang, Chang-yue; Bai, Ming-qiang; Zhou, Si-qi

2018-06-01

We propose a scheme of cyclic joint remote state preparation for three sides, which takes advantage of three GHZ states to compose product state as quantum channel. Suppose there are six legitimate participants, says Alice, Bob, Charlie, David, Emma and Fred in the scheme. It can be shown that Alice and David can remotely prepare a single-qubit state on Bob's side; meanwhile, Bob and Emma can remotely prepare a desired quantum state on Charlie's side, and Charlie and Fred can also remotely prepare a single-qubit state on Alice's side at the same time. Further, it can be achieved in the opposite direction of the cycle by changing the quantum channel. Based on it, we generalize this protocol to N (N≥3) sides utilizing three multi-qubit GHZ-type states as quantum channel. Therefore, the scheme can achieve cyclic joint remote state preparation, which remotely prepares N states in quantum network with N-party, simultaneously. In addition, we consider that the effect of amplitude-damping noise of the initial states is prepared in four different laboratory. Clearly, we use fidelity to describe how much information has been lost in the cyclic process. Our investigation about the effect of noise shows that the preparing of the initial state in different laboratories will affect the loss of information.

DISTRIBUTION OF AQUATIC OFF-CHANNEL HABITATS AND ASSOCIATED RIPARIAN VEGETATION, WILLAMETTE RIVER, OREGON, USA

EPA Science Inventory

The extent of aquatic off-channel habitats such as secondary and side channels, sloughs, and alcoves, have been reduced more than 50% since the 1850s along the upper main stem of the Willamette River, Oregon, USA. Concurrently, the hydrogeomorphic potential, and associated flood...

WATER QUALITY CHANGES IN HYPORHEIC FLOW AT THE AQUATIC-TERRESTRIAL INTERFACE OF A LARGER RIVER

EPA Science Inventory

Exchange between river water and groundwater in hyporheic flow at the aquatic-terrestrial interface can importantly affect water quality and aquatic habitat in the main channel of large rivers and at off-channel sites that include flowing and stagnant side channels. With tracer ...

Validation of High Aspect Ratio Cooling in a 89 kN (20,000 lb(sub f)) Thrust Combustion Chamber

NASA Technical Reports Server (NTRS)

Wadel, Mary F.; Meyer, Michael L.

1996-01-01

In order to validate the benefits of high aspect ratio cooling channels in a large scale rocket combustion chamber, a high pressure, 89 kN (20,000 lbf) thrust, contoured combustion chamber was tested in the NASA Lewis Research Center Rocket Engine Test Facility. The combustion chamber was tested at chamber pressures from 5.5 to 11.0 MPa (800-1600 psia). The propellants were gaseous hydrogen and liquid oxygen at a nominal mixture ratio of six, and liquid hydrogen was used as the coolant. The combustion chamber was extensively instrumented with 30 backside skin thermocouples, 9 coolant channel rib thermocouples, and 10 coolant channel pressure taps. A total of 29 thermal cycles, each with one second of steady state combustion, were completed on the chamber. For 25 thermal cycles, the coolant mass flow rate was equal to the fuel mass flow rate. During the remaining four thermal cycles, the coolant mass flow rate was progressively reduced by 5, 6, 11, and 20 percent. Computer analysis agreed with coolant channel rib thermocouples within an average of 9 percent and with coolant channel pressure drops within an average of 20 percent. Hot-gas-side wall temperatures of the chamber showed up to 25 percent reduction, in the throat region, over that of a conventionally cooled combustion chamber. Reducing coolant mass flow yielded a reduction of up to 27 percent of the coolant pressure drop from that of a full flow case, while still maintaining up to a 13 percent reduction in a hot-gas-side wall temperature from that of a conventionally cooled combustion chamber.

Do preclinical seizure models preselect certain adverse effects of antiepileptic drugs.

PubMed

Meldrum, Brian

2002-06-01

Classical screening tests (maximal electroshock, MES, and threshold pentylenetetrazol, PTZ) employ non-epileptic rodents and identify antiepileptic drugs (AEDs) with mechanisms of action associated with significant CNS side effects. Thus MES identifies drugs acting on Na+ channels that produce cerebellar toxicity. It may be possible to produce novel AEDs more selectively targeted at voltage-sensitive (VS) ion channels. There is little specific evidence for the likely success of this strategy with subunit selective agents targeted at the different VS Na+ channels. Drugs targeted at specific VS Ca++ channels (T, N, P/Q types) may be useful in generalised seizures. There are many as yet unexplored possibilities relating to K+ channels. GABA related drugs acting on PTZ clonic seizures tend to induce sedation and muscle hypotonia. Studies in mice, particularly with knock-in mutations, but also with subunit selective agents acting via the GABA(A) benzodiazepine site, suggest that it is possible to produce agents which do or do not induce particular side effects (sedative, hypnotic, anxiolytic, muscle relaxant, amnesia, anaesthesia). Whether these findings transfer to man has yet to be established. Acquired epilepsy in rodents (e.g. kindling or spontaneous seizures following chemically- or electrically-induced status epilepticus) or acquired epilepsy in man (following prolonged febrile seizures or traumatic brain injury) is associated with multiple changes in the function and subunit composition of ion channels and receptor molecules. Optimal screening of novel AEDs, both for efficacy and side effects, requires models with receptor and ion channel changes similar to those in the target human syndrome.

Hydrological control over stream nitrate loss in an aggrading New Hampshire forest

NASA Astrophysics Data System (ADS)

Daley, R.; Goodale, C. L.; Buso, D.; Driscoll, C. T.; Fuss, C.; Likens, G. E.

2008-12-01

Stream chemistry of a small watershed in the Hubbard Brook Experimental Forest (Watershed 4) displays higher nitrate export than expected for an early successional forest in this region. Within Watershed 4, a small tributary (300 m in length) has a chemical signature far different from the main channel (1000 m in length). Previous monitoring has shown that the pH of the side tributary was significantly higher than that of the main channel and contains detectable levels of nitrate whereas the main channel had no detectable nitrate, and it is suspected that this side tributary significantly contributes to watershed export under base flow conditions. We expected that watershed's dominant water and chemical sources would vary with flow conditions, especially during summer thunderstorms. We hypothesized that the side tributary is the dominant source area under the normal base flow conditions of the summer (usually under 1 L/s) and that the main stem exerts dominance under the high flow conditions brought on by events. Daily water samples were taken throughout summer 2008 with three ISCO automated samplers: One ISCO was placed at the main stem of the stream, a second at the small internal tributary, and a third was placed at the weir. The samples were analyzed for pH, specific conductivity, and ANC, DOC, DON, and major anions and cations. The chemical data was compared to precipitation and rate of watershed flow calculated at each sampling hour to detect associations between chemical dominance and hydrological conditions. Under the base flow conditions of the summer, the chemistry of watershed outflow was dominated by that of the short side tributary, with lower acidity and higher nitrate levels than the longer main channel, but with notable contributions from the main channel. During each of the three high flow events that occurred over the summer, flow in the main channel increased dramatically and flow at the weir corresponded to temporarily increased acidity and decreased ANC. Preliminary nitrate data shows that a dilution response was associated with these events since there were significant declines in the nitrate levels of both the side tributary and watershed export during high flow conditions.

Improving the mixing performance of side channel type micromixers using an optimal voltage control model.

PubMed

Wu, Chien-Hsien; Yang, Ruey-Jen

2006-06-01

Electroosmotic flow in microchannels is restricted to low Reynolds number regimes. Since the inertia forces are extremely weak in such regimes, turbulent conditions do not readily develop, and hence species mixing occurs primarily as a result of diffusion. Consequently, achieving a thorough species mixing generally relies upon the use of extended mixing channels. This paper aims to improve the mixing performance of conventional side channel type micromixers by specifying the optimal driving voltages to be applied to each channel. In the proposed approach, the driving voltages are identified by constructing a simple theoretical scheme based on a 'flow-rate-ratio' model and Kirchhoff's law. The numerical and experimental results confirm that the optimal voltage control approach provides a better mixing performance than the use of a single driving voltage gradient.

Sodium channel slow inactivation as a therapeutic target for myotonia congenita

PubMed Central

Novak, Kevin R; Norman, Jennifer; Mitchell, Jacob R; Pinter, Martin J; Rich, Mark M

2014-01-01

Objective Patients with myotonia congenita have muscle hyperexcitability due to loss-of-function mutations in the chloride channel in skeletal muscle, which causes spontaneous firing of muscle action potentials (myotonia), producing muscle stiffness. In patients, muscle stiffness lessens with exercise, a change known as the warm-up phenomenon. Our goal was to identify the mechanism underlying warm up and to use this information to guide development of novel therapy. Methods To determine the mechanism underlying warm-up, we used a recently discovered drug to eliminate muscle contraction, thus allowing prolonged intracellular recording from individual muscle fibers during induction of warm-up in a mouse model of myotonia congenita. Results Changes in action potentials suggested slow inactivation of sodium channels as an important contributor to warm-up. These data suggested enhancing slow inactivation of sodium channels might offer effective therapy for myotonia. Lacosamide and ranolazine enhance slow inactivation of sodium channels and are FDA-approved for other uses in patients. We compared the efficacy of both drugs to mexiletine, a sodium channel blocker currently used to treat myotonia. In vitro studies suggested both lacosamide and ranolazine were superior to mexiletine. However, in vivo studies in a mouse model of myotonia congenita suggested side effects could limit the efficacy of lacosamide. Ranolazine produced fewer side effects and was as effective as mexiletine at a dose that produced none of mexiletine’s hypoexcitability side effects. Interpretation We conclude ranolazine has excellent therapeutic potential for treatment of patients with myotonia congenita. PMID:25515836

Three dimensional profile measurement using multi-channel detector MVM-SEM

NASA Astrophysics Data System (ADS)

Yoshikawa, Makoto; Harada, Sumito; Ito, Keisuke; Murakawa, Tsutomu; Shida, Soichi; Matsumoto, Jun; Nakamura, Takayuki

2014-07-01

In next generation lithography (NGL) for the 1x nm node and beyond, the three dimensional (3D) shape measurements such as side wall angle (SWA) and height of feature on photomask become more critical for the process control. Until today, AFM (Atomic Force Microscope), X-SEM (cross-section Scanning Electron Microscope) and TEM (Transmission Electron Microscope) tools are normally used for 3D measurements, however, these techniques require time-consuming preparation and observation. And both X-SEM and TEM are destructive measurement techniques. This paper presents a technology for quick and non-destructive 3D shape analysis using multi-channel detector MVM-SEM (Multi Vision Metrology SEM), and also reports its accuracy and precision.

Finite element simulation of the T-shaped ECAP processing of round samples

NASA Astrophysics Data System (ADS)

Shaban Ghazani, Mehdi; Fardi-Ilkhchy, Ali; Binesh, Behzad

2018-05-01

Grain refinement is the only mechanism that increases the yield strength and toughness of the materials simultaneously. Severe plastic deformation is one of the promising methods to refine the microstructure of materials. Among different severe plastic deformation processes, the T-shaped equal channel angular pressing (T-ECAP) is a relatively new technique. In the present study, finite element analysis was conducted to evaluate the deformation behavior of metals during T-ECAP process. The study was focused mainly on flow characteristics, plastic strain distribution and its homogeneity, damage development, and pressing force which are among the most important factors governing the sound and successful processing of nanostructured materials by severe plastic deformation techniques. The results showed that plastic strain is localized in the bottom side of sample and uniform deformation cannot be possible using T-ECAP processing. Friction coefficient between sample and die channel wall has a little effect on strain distributions in mirror plane and transverse plane of deformed sample. Also, damage analysis showed that superficial cracks may be initiated from bottom side of sample and their propagation will be limited due to the compressive state of stress. It was demonstrated that the V shaped deformation zone are existed in T-ECAP process and the pressing load needed for execution of deformation process is increased with friction.

Cadmium-cysteine coordination in the BK inner pore region and its structural and functional implications.

PubMed

Zhou, Yu; Xia, Xiao-Ming; Lingle, Christopher J

2015-04-21

To probe structure and gating-associated conformational changes in BK-type potassium (BK) channels, we examined consequences of Cd(2+) coordination with cysteines introduced at two positions in the BK inner pore. At V319C, the equivalent of valine in the conserved Kv proline-valine-proline (PVP) motif, Cd(2+) forms intrasubunit coordination with a native glutamate E321, which would place the side chains of V319C and E321 much closer together than observed in voltage-dependent K(+) (Kv) channel structures, requiring that the proline between V319C and E321 introduces a kink in the BK S6 inner helix sharper than that observed in Kv channel structures. At inner pore position A316C, Cd(2+) binds with modest state dependence, suggesting the absence of an ion permeation gate at the cytosolic side of BK channel. These results highlight fundamental structural differences between BK and Kv channels in their inner pore region, which likely underlie differences in voltage-dependent gating between these channels.

Estuarine Channel Evolution in Response to Closure of Secondary Basins: An Observational and Morphodynamic Modeling Study of the Western Scheldt Estuary

NASA Astrophysics Data System (ADS)

Nnafie, A.; Van Oyen, T.; De Maerschalck, B.; van der Vegt, M.; Wegen, M. van der

2018-01-01

The fringes of estuaries are often characterized by the presence of side embayments (secondary basins), with dimensions in the order of hundreds of meters to tens of kilometers. The presence of secondary basins significantly alters the hydrodynamic and sediment characteristics in the main estuary, implying that loss of secondary basin area due to human interventions might affect the estuarine morphodynamics. Analysis of historical bathymetric data of the Western Scheldt Estuary (Netherlands) suggests that closure of its secondary basins has triggered the observed lateral displacement of the nearby channels. This analysis motivated investigation of the impact of secondary basins on decadal evolution of estuarine channels, using the numerical model Delft3D. Model results show that channels that form near a secondary basin are located farther away from the bank of the estuary with respect to their positions in the case without a basin. Overall, results in cases with two or three basins are similar to those in case with one single basin. The wider the basin, the farther away the nearby channel forms. Removing a secondary basin causes a lateral displacement of the nearby channel toward the bank, indicating that the observed lateral displacement of channels in the Western Scheldt is triggered by closure of its secondary basins. The physical explanation is that tidal currents in the main estuary are weaker and more rotary near secondary basins, favoring sediment deposition and shoal development at these locations. Model results are particularly relevant for estuaries with moderate to high friction and converging width.

A method to estimate canal leakage to the Biscayne Aquifer, Dade County, Florida

USGS Publications Warehouse

Chin, D.A.

1990-01-01

The leakage characteristics of channels that partially penetrate the Biscayne aquifer and have reduced bed permeability were studied. Leakage characteristics were described in terms of a reach transmissivity-defined as the volume flow rate out of the channel per unit length of the channel per unit drawdown, where drawdown is defined as the difference in altitude between the water surface in the canal and the water table in the adjacent aquifer. A theoretical expression was developed to relate the reach transmissivity to the transmissivity of the formation, mean channel width, distance of drawdown measurement from the channel centerline, ratio of drawdowns on both sides of the channel, and local reach transmissivity associated with reduced bed permeability. This theoretical expression was verified using a fine-scale numerical model, which gave accurate results when drawdowns were measured beyond 10 aquifer depths from the side of the channel. Using the theoretical formulation, it is shown that the reach transmissivity employed in regional ground-water models, which are based on average drawdowns within a cell, depends on the size of the cell as well as the transmissivity of the formation, channel width, and local reach transmissivity due to reduced bed permeability. The theoretical reach transmissivity function was compared with field measurements at L-31N Canal and Snapper Creek Extension Canal in Dade County, Florida. Analyses of the data for both canals showed good agreement between the estimated and measured reach transmissivities. At L- 31N Canal, field measurements indicated that the local reach transmissivity was relatively uniform over a 2-mile reach of the channel (averaging 630 cubic feet per second per mile per foot), and the formation transmissivity was 1.8 x106 feet squared per day. At Snapper Creek Extension Canal, an approximate analysis was necessary due to the inability of the acoustic velocity meter to measure very low water velocities in the channel. Assuming an aquifer transmissivity of 1 x 106 feet squared per day, drawdown measurements indicated that the local reach transmissivity was about 400 cubic feet per second per mile per foot. The theoretical relation, combined with the local reach transmissivity and formation transmissivity, was sufficient to predict the leakage out of L-31N Canal and Snapper Creek Extension Canal for any drawdown scenario.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R.; Torczynski, John R.; Brady, Patrick V.; Gallis, Michail; Brooks, Carlton F.

2014-06-17

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Lanicemine: a low-trapping NMDA channel blocker produces sustained antidepressant efficacy with minimal psychotomimetic adverse effects.

PubMed

Sanacora, G; Smith, M A; Pathak, S; Su, H-L; Boeijinga, P H; McCarthy, D J; Quirk, M C

2014-09-01

Ketamine, an N-methyl-D-aspartate receptor (NMDAR) channel blocker, has been found to induce rapid and robust antidepressant-like effects in rodent models and in treatment-refractory depressed patients. However, the marked acute psychological side effects of ketamine complicate the interpretation of both preclinical and clinical data. Moreover, the lack of controlled data demonstrating the ability of ketamine to sustain the antidepressant response with repeated administration leaves the potential clinical utility of this class of drugs in question. Using quantitative electroencephalography (qEEG) to objectively align doses of a low-trapping NMDA channel blocker, AZD6765 (lanicemine), to that of ketamine, we demonstrate the potential for NMDA channel blockers to produce antidepressant efficacy without psychotomimetic and dissociative side effects. Furthermore, using placebo-controlled data, we show that the antidepressant response to NMDA channel blockers can be maintained with repeated and intermittent drug administration. Together, these data provide a path for the development of novel glutamatergic-based therapeutics for treatment-refractory mood disorders.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R; Torczynski, John R; Brady, Patrick V; Gallis, Michail; Brooks, Carlton F

2013-09-17

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Process and apparatus for separation of components of a gas stream

DOEpatents

Bryan, Charles R; Torczynski, John R; Brady, Patrick V; Gallis, Michail; Brooks, Carlton F

2013-11-19

A process and apparatus for separating a gas mixture comprising providing a slot in a gas separation channel (conceptualized as a laterally elongated Clusius-Dickel column), having a length through which a net cross-flow of the gas mixture may be established; applying a higher temperature to one side of the channel and a lower temperature on an opposite side of the channel thereby causing thermal-diffusion and buoyant-convection flow to occur in the slot; and establishing a net cross-flow of a gas mixture comprising at least one higher density gas component and at least one lower density gas component along the length of the slot, wherein the cross-flow causes, in combination with the convection flow, a spiraling flow in the slot; and wherein the spiral flow causes an increasing amount of separation of the higher density gas from the lower density gas along the length of the channel. The process may use one or more slots and/or channels.

Streamflow and streambed scour in 2010 at bridge 339, Copper River, Alaska

USGS Publications Warehouse

Conaway, Jeffrey S.; Brabets, Timothy P.

2011-01-01

The distribution of the Copper River's discharge through the bridges was relatively stable until sometime between 1969-70 and 1982-85. The majority of the total Copper River discharge in 1969-70 passed through three bridges on the western side of the delta, but by 1982-1985, 25 to 62 percent of the flow passed through bridge 342 on the eastern side of the Copper River Delta. In 2004, only 8 percent of the flow passed through the western bridges, while 90 percent of the discharge flowed through two bridges on the eastern side of the delta. Migration of the river across the delta and redistribution of discharge has resulted in streambed scour at some bridges, overtopping of the road during high flows, prolonged highway closures, and formation of new channels through forests. Scour monitoring at the eastern bridges has recorded as much as 44 feet of fill at one pier and 33 feet of scour at another. In 2009, flow distribution began to shift from the larger bridge 342 to bridge 339. In 2010, flow in excess of four times the design discharge scoured the streambed at bridge 339 to a level such that constant on-site monitoring was required to evaluate the potential need for bridge closure. In 2010, instantaneous flow through bridge 339 was never less than 30 percent and was as high as 49 percent of the total Copper River discharge. The percentage of flow through bridge 339 decreased when the overall Copper River discharge increased. The increased discharge through bridge 339 is attributed to a shift in the approach channel 3,500 feet upstream. Bridge channel alignment and analysis of flow distribution as of October 2010 indicate these hydrologic hazards will persist in 2011.

A Compact and Low Power RO PUF with High Resilience to the EM Side-Channel Attack and the SVM Modelling Attack of Wireless Sensor Networks

PubMed Central

Cao, Yuan; Ye, Wenbin; Han, Qingbang; Pan, Xiaofang

2018-01-01

Authentication is a crucial security service for the wireless sensor networks (WSNs) in versatile domains. The deployment of WSN devices in the untrusted open environment and the resource-constrained nature make the on-chip authentication an open challenge. The strong physical unclonable function (PUF) came in handy as light-weight authentication security primitive. In this paper, we present the first ring oscillator (RO) based strong physical unclonable function (PUF) with high resilience to both the electromagnetic (EM) side-channel attack and the support vector machine (SVM) modelling attack. By employing an RO based PUF architecture with the current starved inverter as the delay cell, the oscillation power is significantly reduced to minimize the emitted EM signal, leading to greatly enhanced immunity to the EM side-channel analysis attack. In addition, featuring superior reconfigurability due to the conspicuously simplified circuitries, the proposed implementation is capable of withstanding the SVM modelling attack by generating and comparing a large number of RO frequency pairs. The reported experimental results validate the prototype of a 9-stage RO PUF fabricated using standard 65 nm complementary-metal-oxide-semiconductor (CMOS) process. Operating at the supply voltage of 1.2 V and the frequency of 100 KHz, the fabricated RO PUF occupies a compact silicon area of 250 μm2 and consumes a power as low as 5.16 μW per challenge-response pair (CRP). Furthermore, the uniqueness and the worst-case reliability are measured to be 50.17% and 98.30% for the working temperature range of −40∼120 ∘C and the supply voltage variation of ±2%, respectively. Thus, the proposed PUF is applicable for the low power, low cost and secure WSN communications. PMID:29360790

A Compact and Low Power RO PUF with High Resilience to the EM Side-Channel Attack and the SVM Modelling Attack of Wireless Sensor Networks.

PubMed

Cao, Yuan; Zhao, Xiaojin; Ye, Wenbin; Han, Qingbang; Pan, Xiaofang

2018-01-23

Authentication is a crucial security service for the wireless sensor networks (WSNs) in versatile domains. The deployment of WSN devices in the untrusted open environment and the resource-constrained nature make the on-chip authentication an open challenge. The strong physical unclonable function (PUF) came in handy as light-weight authentication security primitive. In this paper, we present the first ring oscillator (RO) based strong physical unclonable function (PUF) with high resilience to both the electromagnetic (EM) side-channel attack and the support vector machine (SVM) modelling attack. By employing an RO based PUF architecture with the current starved inverter as the delay cell, the oscillation power is significantly reduced to minimize the emitted EM signal, leading to greatly enhanced immunity to the EM side-channel analysis attack. In addition, featuring superior reconfigurability due to the conspicuously simplified circuitries, the proposed implementation is capable of withstanding the SVM modelling attack by generating and comparing a large number of RO frequency pairs. The reported experimental results validate the prototype of a 9-stage RO PUF fabricated using standard 65 nm complementary-metal-oxide-semiconductor (CMOS) process. Operating at the supply voltage of 1.2 V and the frequency of 100 KHz, the fabricated RO PUF occupies a compact silicon area of 250 μ m 2 and consumes a power as low as 5.16 μ W per challenge-response pair (CRP). Furthermore, the uniqueness and the worst-case reliability are measured to be 50.17% and 98.30% for the working temperature range of -40∼120 ∘ C and the supply voltage variation of ±2%, respectively. Thus, the proposed PUF is applicable for the low power, low cost and secure WSN communications.

Channel-Based Key Generation for Encrypted Body-Worn Wireless Sensor Networks.

PubMed

Van Torre, Patrick

2016-09-08

Body-worn sensor networks are important for rescue-workers, medical and many other applications. Sensitive data are often transmitted over such a network, motivating the need for encryption. Body-worn sensor networks are deployed in conditions where the wireless communication channel varies dramatically due to fading and shadowing, which is considered a disadvantage for communication. Interestingly, these channel variations can be employed to extract a common encryption key at both sides of the link. Legitimate users share a unique physical channel and the variations thereof provide data series on both sides of the link, with highly correlated values. An eavesdropper, however, does not share this physical channel and cannot extract the same information when intercepting the signals. This paper documents a practical wearable communication system implementing channel-based key generation, including an implementation and a measurement campaign comprising indoor as well as outdoor measurements. The results provide insight into the performance of channel-based key generation in realistic practical conditions. Employing a process known as key reconciliation, error free keys are generated in all tested scenarios. The key-generation system is computationally simple and therefore compatible with the low-power micro controllers and low-data rate transmissions commonly used in wireless sensor networks.

Development of a microfluidic device for cell concentration and blood cell-plasma separation.

PubMed

Maria, M Sneha; Kumar, B S; Chandra, T S; Sen, A K

2015-12-01

This work presents design, fabrication and test of a microfluidic device which employs Fahraeus-Lindqvist and Zweifach-Fung effects for cell concentration and blood cell-plasma separation. The device design comprises a straight main channel with a series of branched channels placed symmetrically on both sides of the main channel. The design implements constrictions before each junction (branching point) in order to direct cells that would have migrated closer to the wall (naturally or after liquid extraction at a junction) towards the centre of the main channel. Theoretical and numerical analysis are performed for design of the microchannel network to ensure that a minimum flow rate ratio (of 2.5:1, main channel-to-side channels) is maintained at each junction and predict flow rate at the plasma outlet. The dimensions and location of the constrictions were determined using numerical simulations. The effect of presence of constrictions before the junctions was demonstrated by comparing the performances of the device with and without constrictions. To demonstrate the performance of the device, initial experiments were performed with polystyrene microbeads (10 and 15 μm size) and droplets. Finally, the device was used for concentration of HL60 cells and separation of plasma and cells in diluted blood samples. The cell concentration and blood-plasma purification efficiency was quantified using Haemocytometer and Fluorescence-Activated Cell Sorter (FACS). A seven-fold cell concentration was obtained with HL60 cells and a purification efficiency of 70 % and plasma recovery of 80 % was observed for diluted (1:20) blood sample. FACS was used to identify cell lysis and the cell viability was checked using Trypan Blue test which showed that more than 99 % cells are alive indicating the suitability of the device for practical use. The proposed device has potential to be used as a sample preparation module in lab on chip based diagnostic platforms.

Modeling the Effects of Connecting Side Channels to the Long Tom River, Oregon

NASA Astrophysics Data System (ADS)

Appleby, C.; McDowell, P. F.

2015-12-01

The lower Long Tom River is a heavily managed, highly modified stream in the southwestern Willamette Valley with many opportunities for habitat improvements and river restoration. In the 1940s and 1950s, the US Army Corps of Engineers dramatically altered this river system by constructing the Fern Ridge Dam and three, large drop structures, converting the River from a highly sinuous channel to a straight, channelized stream that is interrupted by these grade control structures, and removed the majority of the riparian vegetation. As a result, juvenile spring Chinook salmon are no longer found in the Watershed and the local population of coastal cutthroat trout face limited aquatic habitat. When the river was channelized, long sections of the historical channel were left abandoned on the floodplain. Reconnecting these historical channels as side channels may improve the quality and quantity of aquatic habitat and could allow fish passage around current barriers. However, such construction may also lead to undesirable threats to infrastructure and farmland. This study uses multiple HEC-RAS models to determine the impact of reconnecting two historical channels to the lower Long Tom River by quantifying the change in area of flood inundation and identifying infrastructure in jeapordy given current and post-restoration conditions for 1.5, 5, 10, and 25-year flood discharges. Bathymetric data from ADCP and RTK-GPS surveys has been combined with LiDAR-derived topographic data to create continuous elevation models. Several types of side channel connections are modeled in order to determine which type of connection will result in both the greatest quantity of accessible habitat and the fewest threats to public and private property. In the future, this study will also consider the change in the quantity of physical salmonid habitat and map the areas prone to sedimentation and erosion using CEASAR and PHABSIM tools.

Probing membrane permeabilization by the antimicrobial peptide distinctin in mercury-supported biomimetic membranes.

PubMed

Becucci, Lucia; Papini, Martina; Mullen, Daniel; Scaloni, Andrea; Veglia, Gianluigi; Guidelli, Rolando

2011-11-01

The mechanism of membrane permeabilization by the antimicrobial peptide distinctin was investigated by using two different mercury-supported biomimetic membranes, namely a lipid self-assembled monolayer and a lipid bilayer tethered to the mercury surface through a hydrophilic spacer (tethered bilayer lipid membrane: tBLM). Incorporation of distinctin into a lipid monolayer from its aqueous solution yields rapidly ion channels selective toward inorganic cations, such as Tl(+) and Cd(2+). Conversely, its incorporation in a tBLM allows the formation of ion channels permeable to potassium ions only at non-physiological transmembrane potentials, more negative than -340mV. These channels, once formed, are unstable at less negative transmembrane potentials. The kinetics of their formation is consistent with the disruption of distinctin clusters adsorbed on top of the lipid bilayer, incorporation of the resulting monomers and their aggregation into hydrophilic pores by a mechanism of nucleation and growth. Comparing the behavior of distinctin in tBLMs with that in conventional black lipid membranes strongly suggests that distinctin channel formation in lipid bilayer requires the partitioning of distinctin molecules between the two sides of the lipid bilayer. We can tentatively hypothesize that an ion channel is formed when one distinctin cluster on one side of the lipid bilayer matches another one on the opposite side. Copyright © 2011 Elsevier B.V. All rights reserved.

Optical spatial solitons at the interface between two dissimilar periodic media: theory and experiment.

PubMed

Suntsov, S; Makris, K G; Christodoulides, D N; Stegeman, G I; Morandotti, R; Volatier, M; Aimez, V; Arès, R; Yang, E H; Salamo, G

2008-07-07

Discrete spatial solitons traveling along the interface between two dissimilar one-dimensional arrays of waveguides were observed for the first time. Two interface solitons were found theoretically, each one with a peak in a different boundary channel. One evolves into a soliton from a linear mode at an array separation larger than a critical separation where-as the second soliton always exhibits a power threshold. These solitons exhibited different power thresholds which depended on the characteristics of the two lattices. For excitation of single channels near and at the boundary, the evolution behavior with propagation distance indicates that the solitons peaked near and at the interface experience an attractive potential on one side of the boundary, and a repulsive one on the opposite side. The power dependence of the solitons at variable distance from the boundary was found to be quite different on opposite sides of the interface and showed evidence for soliton switching between channels with increasing input power.

Detection of infectious haematopoietic necrosis virus in river water and demonstration of waterborne transmission

USGS Publications Warehouse

Mulcahy, D.; Pascho, R.J.; Jenes, C.K.

1983-01-01

In a study of the possible role of waterborne infectious haematopoietic necrosis virus in transmission of the disease among spawning sockeye salmon, Oncorhynchus nerka (Walbaum), both infection rates and virus titres were higher in fish held at high density in a side channel than in fish in the adjacent river. Virus was never isolated from river water, but was found in water from the side channel at levels ranging from 32.5 to 1600 plaque-forming units (p.f.u.)/ml. Uninfected yearling sockeye salmon held in a box in the side channel developed localized gill infections with IHN virus. The disease did not progress to the viscera until a threshold titre of about 105 p.f.u./g was reached in the gill. The effectiveness of the gill as a barrier limiting development of systemic infections means that waterborne IHN virus probably does not greatly increase the infection rate in a sockeye salmon population during spawning.

Detection of infectious hematopoietic necrosis virus in river water and demonstration of waterborne transmission

USGS Publications Warehouse

Mulcahy, Daniel M.; Pascho, Ronald J.; Jenes, C.K.

1983-01-01

In a study of the possible role of waterborne infectious haematopoietic necrosis virus in transmission of the disease among spawning sockeye salmon, Oncorhynchus nerka (Walbaum), both infection rates and virus titres were higher in fish held at high density in a side channel than in fish in the adjacent river. Virus was never isolated from river water, but was found in water from the side channel at levels ranging from 32.5 to 1600 plaque-forming units (p.f.u.)/ml. Uninfected yearling sockeye salmon held in a box in the side channel developed localized gill infections with IHN virus. The disease did not progress to the viscera until a threshold titre of about 105 p.f.u./g was reached in the gill. The effectiveness of the gill as a barrier limiting development of systemic infections means that waterborne IHN virus probably does not greatly increase the infection rate in a sockeye salmon population during spawning.

22. SIDE WALL CONSTRUCTION, NORTH TRAINING WALL, LOOKING WEST FROM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. SIDE WALL CONSTRUCTION, NORTH TRAINING WALL, LOOKING WEST FROM THE SAME POINT AS VIEW NO. 21. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

The neutron channeling phenomenon.

PubMed

Khanouchi, A; Sabir, A; Boulkheir, M; Ichaoui, R; Ghassoun, J; Jehouani, A

1997-01-01

Shields, used for protection against radiation, are often pierced with vacuum channels for passing cables and other instruments for measurements. The neutron transmission through these shields is an unavoidable phenomenon. In this work we study and discuss the effect of channels on neutron transmission through shields. We consider an infinite homogeneous slab, with a fixed thickness (20 lambda, with lambda the mean free path of the neutron in the slab), which contains a vacuum channel. This slab is irradiated with an infinite source of neutrons on the left side and on the other side (right side) many detectors with windows equal to 2 lambda are placed in order to evaluate the neutron transmission probabilities (Khanouchi, A., Aboubekr, A., Ghassoun, J. and Jehouani, A. (1994) Rencontre Nationale des Jeunes Chercheurs en Physique. Casa Blanca Maroc; Khanouchi, A., Sabir, A., Ghassoun, J. and Jehouani, A. (1995) Premier Congré International des Intéractions Rayonnements Matière. Eljadida Maroc). The neutron history within the slab is simulated by the Monte Carlo method (Booth, T. E. and Hendricks, J. S. (1994) Nuclear Technology 5) and using the exponential biasing technique in order to improve the Monte Carlo calculation (Levitt, L. B. (1968) Nuclear Science and Engineering 31, 500-504; Jehouani, A., Ghassoun, J. and Aboubker, A. (1994) In Proceedings of the 6th International Symposium on Radiation Physics, Rabat, Morocco). Then different geometries of the vacuum channel have been studied. For each geometry we have determined the detector response and calculated the neutron transmission probability for different detector positions. This neutron transmission probability presents a peak for the detectors placed in front of the vacuum channel. This study allowed us to clearly identify the neutron channeling phenomenon. One application of our study is to detect vacuum defects in materials.

The effect of basal channels on oceanic ice-shelf melting

NASA Astrophysics Data System (ADS)

Millgate, Thomas; Holland, Paul R.; Jenkins, Adrian; Johnson, Helen L.

2013-12-01

The presence of ice-shelf basal channels has been noted in a number of Antarctic and Greenland ice shelves, but their impact on basal melting is not fully understood. Here we use the Massachusetts Institute of Technology general circulation model to investigate the effect of ice-shelf basal channels on oceanic melt rate for an idealized ice shelf resembling the floating tongue of Petermann Glacier in Greenland. The introduction of basal channels prevents the formation of a single geostrophically balanced boundary current; instead the flow is diverted up the right-hand (Coriolis-favored) side of each channel, with a return flow in the opposite direction on the left-hand side. As the prescribed number of basal channels is increased the mean basal melt rate decreases, in agreement with previous studies. For a small number of relatively wide channels the subice flow is found to be a largely geostrophic horizontal circulation. The reduction in melt rate is then caused by an increase in the relative contribution of weakly melting channel crests and keels. For a larger number of relatively narrow channels, the subice flow changes to a vertical overturning circulation. This change in circulation results in a weaker sensitivity of melt rates to channel size. The transition between the two regimes is governed by the Rossby radius of deformation. Our results explain why basal channels play an important role in regulating basal melting, increasing the stability of ice shelves.

Blade for a gas turbine

DOEpatents

Liang, George

2010-10-26

A blade is provided for a gas turbine. The blade comprises a main body comprising a cooling fluid entrance channel; a cooling fluid collector in communication with the cooling fluid entrance channel; a plurality of side channels extending through an outer wall of the main body and communicating with the cooling fluid collector and a cooling fluid cavity; a cooling fluid exit channel communicating with the cooling fluid cavity; and a plurality of exit bores extending from the cooling fluid exit channel through the main body outer wall.

Development of Switchable Polarity Solvent Draw Solutes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, Aaron D.

Results of a computational fluid dynamic (CFD) study of flow and heat transfer in a printed circuit heat exchanger (PCHE) geometry are presented. CFD results obtained from a two-plate model are compared to corresponding experimental results for the validation. This process provides the basis for further application of the CFD code to PCHE design and performance analysis in a variety of internal flow geometries. As a part of the code verification and validation (V&V) process, CFD simulation of a single semicircular straight channel under laminar isothermal conditions was also performed and compared to theoretical results. This comparison yielded excellent agreementmore » with the theoretical values. The two-plate CFD model based on the experimental PCHE design overestimated the effectiveness and underestimated the pressure drop. However, it is found that the discrepancy between the CFD result and experimental data was mainly caused by the uncertainty in the geometry of heat exchanger during the fabrication. The CFD results obtained using a slightly smaller channel diameter yielded good agreement with the experimental data. A separate investigation revealed that the average channel diameter of the OSU PCHE after the diffusion-bonding was 1.93 mm on the cold fluid side and 1.90 mm on the hot fluid side which are both smaller than the nominal design value. Consequently, the CFD code was shown to have sufficient capability to evaluate the heat exchanger thermal-hydraulic performance.« less

Fractographic Investigation of Micromechanisms of Fracture in Alumina Ceramics

DTIC Science & Technology

1981-11-30

mechanisms flaw linking work of fracture electron channeling crack branching environmental effects 20. A07 ACT (Continue an reverse side Of necessary and...CLASSIFICATION OF THIS PAGE(I hm Date "ftn.,a environments using multiple techniques such as SEM, TEM, selected area electron channeling , and...94 Selected area electron channeling (SAEC) .. .... ........ 99 V. CONCLUSIONS. .. ............................ 100 VI. REFERENCES

33 CFR 162.260 - Channel leading to San Juan Harbor, P.R.; use, administration, and navigation.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., and navigation. (a) Steamers passing dredge engaged in improving the channel shall not have a speed... dredge anchors. (b) Vessels using the channel shall pass the dredge on the side designated from the dredge by the signals prescribed in paragraph (c) of this section. (c) Dredge shall display the red flag...

33 CFR 162.260 - Channel leading to San Juan Harbor, P.R.; use, administration, and navigation.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., and navigation. (a) Steamers passing dredge engaged in improving the channel shall not have a speed... dredge anchors. (b) Vessels using the channel shall pass the dredge on the side designated from the dredge by the signals prescribed in paragraph (c) of this section. (c) Dredge shall display the red flag...

Process for off-gas particulate removal and apparatus therefor

DOEpatents

Carl, D.E.

1997-10-21

In the event of a breach in the off-gas line of a melter operation requiring closure of the line, a secondary vessel vent line is provided with a particulate collector utilizing atomization for removal of large particulates from the off-gas. The collector receives the gas containing particulates and directs a portion of the gas through outer and inner annular channels. The collector further receives a fluid, such as water, which is directed through the outer channel together with a second portion of the particulate-laden gas. The outer and inner channels have respective ring-like termination apertures concentrically disposed adjacent one another on the outer edge of the downstream side of the particulate collector. Each of the outer and inner channels curves outwardly away from the collector`s centerline in proceeding toward the downstream side of the collector. Gas flow in the outer channel maintains the fluid on the channel`s wall in the form of a ``wavy film,`` while the gas stream from the inner channel shears the fluid film as it exits the outer channel in reducing the fluid to small droplets. Droplets formed by the collector capture particulates in the gas stream by one of three mechanisms: impaction, interception or Brownian diffusion in removing the particulates. The particulate-laden droplets are removed from the fluid stream by a vessel vent condenser or mist eliminator. 4 figs.

22. View of Clark Fork Vehicle Bridge facing downwest side. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. View of Clark Fork Vehicle Bridge facing down-west side. Looking at road deck and vertical laced channel. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

Catalytic bipolar interconnection plate for use in a fuel cell

DOEpatents

Lessing, Paul A.

1996-01-01

A bipolar interconnection plate for use between adjacent fuel cell units in a stacked fuel cell assembly. Each plate is manufactured from an intermetallic composition, examples of which include NiAl or Ni.sub.3 Al which can catalyze steam reforming of hydrocarbons. Distributed within the intermetallic structure of the plate is a ceramic filler composition. The plate includes a first side with gas flow channels therein and a second side with fuel flow channels therein. A protective coating is applied to the first side, with exemplary coatings including strontium-doped or calcium-doped lanthanum chromite. To produce the plate, Ni and Al powders are combined with the filler composition, compressed at a pressure of about 10,000-30,000 psi, and heated to about 600.degree.-1000.degree. C. The coating is then applied to the first side of the completed plate using liquid injection plasma deposition or other deposition techniques.

From Modules to a Generator: An Integrated Heat Exchanger Concept for Car Applications of a Thermoelectric Generator

NASA Astrophysics Data System (ADS)

Bosch, Henry

2016-03-01

A heat exchanger concept for a thermoelectric generator with integrated planar modules for passenger car applications is introduced. The module housings, made of deep drawn stainless steel sheet metal, are brazed onto the exhaust gas channel to achieve an optimal heat transfer on the hot side of the modules. The cooling side consists of winding fluid channels, which are mounted directly onto the cold side of the modules. Only a thin foil separates the cooling media from the modules for an almost direct heat contact on the cooling side. Thermoelectric generators with up to 20 modules made of PbTe and Bi2Te3, respectively, are manufactured and tested on a hot gas generator to investigate electrical power output and performance of the thermoelectric generator. The proof of concept of the light weight heat exchanger design made of sheet metal with integrated modules is positively accomplished.

Catalytic bipolar interconnection plate for use in a fuel cell

DOEpatents

Lessing, P.A.

1996-03-05

A bipolar interconnection plate is described for use between adjacent fuel cell units in a stacked fuel cell assembly. Each plate is manufactured from an intermetallic composition, examples of which include NiAl or Ni{sub 3}Al which can catalyze steam reforming of hydrocarbons. Distributed within the intermetallic structure of the plate is a ceramic filler composition. The plate includes a first side with gas flow channels therein and a second side with fuel flow channels therein. A protective coating is applied to the first side, with exemplary coatings including strontium-doped or calcium-doped lanthanum chromite. To produce the plate, Ni and Al powders are combined with the filler composition, compressed at a pressure of about 10,000--30,000 psi, and heated to about 600--1000 C. The coating is then applied to the first side of the completed plate using liquid injection plasma deposition or other deposition techniques. 6 figs.

Sources of debris flow material in burned areas

USGS Publications Warehouse

Santi, P.M.; deWolfe, V.G.; Higgins, J.D.; Cannon, S.H.; Gartner, J.E.

2008-01-01

The vulnerability of recently burned areas to debris flows has been well established. Likewise, it has been shown that many, if not most, post-fire debris flows are initiated by runoff and erosion and grow in size through erosion and scour by the moving debris flow, as opposed to landslide-initiated flows with little growth. To better understand the development and character of these flows, a study has been completed encompassing 46 debris flows in California, Utah, and Colorado, in nine different recently burned areas. For each debris flow, progressive debris production was measured at intervals along the length of the channel, and from these measurements graphs were developed showing cumulative volume of debris as a function of channel length. All 46 debris flows showed significant bulking by scour and erosion, with average yield rates for each channel ranging from 0.3 to 9.9??m3 of debris produced for every meter of channel length, with an overall average value of 2.5??m3/m. Significant increases in yield rate partway down the channel were identified in 87% of the channels, with an average of a three-fold increase in yield rate. Yield rates for short reaches of channels (up to several hundred meters) ranged as high as 22.3??m3/m. Debris was contributed from side channels into the main channels for 54% of the flows, with an average of 23% of the total debris coming from those side channels. Rill erosion was identified for 30% of the flows, with rills contributing between 0.1 and 10.5% of the total debris, with an average of 3%. Debris was deposited as levees in 87% of the flows, with most of the deposition occurring in the lower part of the basin. A median value of 10% of the total debris flow was deposited as levees for these cases, with a range from near zero to nearly 100%. These results show that channel erosion and scour are the dominant sources of debris in burned areas, with yield rates increasing significantly partway down the channel. Side channels are much more important sources of debris than rills. Levees are very common, but the size and effect on the amount of debris that reaches a canyon mouth is highly variable. ?? 2007 Elsevier B.V. All rights reserved.

Barrier island breach evolution: Alongshore transport and bay-ocean pressure gradient interactions

USGS Publications Warehouse

Safak, Ilgar; Warner, John C.; List, Jeffrey

2016-01-01

Physical processes controlling repeated openings and closures of a barrier island breach between a bay and the open ocean are studied using aerial photographs and atmospheric and hydrodynamic observations. The breach site is located on Pea Island along the Outer Banks, separating Pamlico Sound from the Atlantic Ocean. Wind direction was a major control on the pressure gradients between the bay and the ocean to drive flows that initiate or maintain the breach opening. Alongshore sediment flux was found to be a major contributor to breach closure. During the analysis period from 2011 to 2016, three hurricanes had major impacts on the breach. First, Hurricane Irene opened the breach with wind-driven flow from bay to ocean in August 2011. Hurricane Sandy in October 2012 quadrupled the channel width from pressure gradient flows due to water levels that were first higher on the ocean side and then higher on the bay side. The breach closed sometime in Spring 2013, most likely due to an event associated with strong alongshore sediment flux but minimal ocean-bay pressure gradients. Then, in July 2014, Hurricane Arthur briefly opened the breach again from the bay side, in a similar fashion to Irene. In summary, opening and closure of breaches are shown to follow a dynamic and episodic balance between along-channel pressure gradient driven flows and alongshore sediment fluxes.

Environment-friendly reduction of flood risk and infrastructure damage in a mountain river: Case study of the Czarny Dunajec

NASA Astrophysics Data System (ADS)

Mikuś, Paweł; Wyżga, Bartłomiej; Radecki-Pawlik, Artur; Zawiejska, Joanna; Amirowicz, Antoni; Oglęcki, Paweł

2016-11-01

Migration of a mountain river channel may cause erosional risk to infrastructure or settlements on the valley floor. Following a flood of 2010, a cutbank in one of the bends of the main channel of the Czarny Dunajec, Polish Carpathians, approached a local road by 50 m. To arrest the erosion of the laterally migrating channel, water authorities planned construction of a ditch cutting the forested neck of the bend, reinforcement of the ditch banks, and damming the main channel with a boulder groyne. In order to avoid channelization of the highly valued, multithread river reach that would deteriorate its ecological status and cause increased flood risk to downstream reaches, an alternative approach to prevent bank erosion was proposed. The new scheme, applied in 2011, included opening of the inlets to inactive side braids located by the neck of the bend of the main channel. This solution reestablished the flow in the steeper low-flow channels, allowing us to expect a cutoff and abandonment of the main channel during subsequent floods. Gravelly deflectors were constructed directly below the inlets to the reactivated side channels to divert the flow into the channels and prevent the water from entering the main channel. Hydraulic measurements performed before and after the implementation of the scheme confirmed that it enabled shifting the main water current, with the highest average velocity and bed shear stress, from the braid closest to the road to the most distant braid. Similar surveys of fish and benthic macroinvertebrate communities indicated that flow reactivation in the side channels was beneficial for these groups of river biota, increasing their abundance and taxonomic richness in the reach. Not only was the implemented solution significantly less expensive, but it also enhanced ecological functions of the multithread channel and the variability of physical habitat conditions and maintained the role of the reach as a wood debris trap. However, avulsion of the main channel in the river bend immediately upstream during the flood in May 2014 again caused erosional risk to the road, although at another location. This indicates that with the highly unstable, multithread channel pattern of the Czarny Dunajec, the best practice of river maintenance in a relatively unmanaged valley reach would be allowing free channel migration within the floodplain area and reinforcing, where necessary, the boundary between the erodible river corridor and the managed terrace.

33 CFR 66.10-15 - Aids to navigation.

Code of Federal Regulations, 2010 CFR

2010-07-01

... may be used singly in staggered fashion on alternate sides of the channel provided they are spaced at... should pass between the buoys. (d) Where there is no well-defined channel or when a body of water is...

33 CFR 66.10-15 - Aids to navigation.

Code of Federal Regulations, 2011 CFR

2011-07-01

... may be used singly in staggered fashion on alternate sides of the channel provided they are spaced at... should pass between the buoys. (d) Where there is no well-defined channel or when a body of water is...

Morphology and growth pattern of Amazon deep-sea fan: a computer-processed GLORIA side-scan mosaic

DOE Office of Scientific and Technical Information (OSTI.GOV)

Flood, R.D.; Damuth, J.E.

1984-04-01

Deep-sea fans have become increasingly important targets for exploration because of their favorable facies associations. A better understanding of deep-sea fans is needed to successfully exploit these complex sediment bodies. Recent studies of the Amazon fan, using long-range side-scan sonar (GLORIA) and single-channel seismic data, provide an overall view of channel patterns of this fan and demonstrate the relationship between successive channel/levee systems. The digitally collected GLORIA data have been computer processed to produce a mosaic of the fan. Computer processing has corrected the records for slant range and ship navigation, and targets have been enhanced. Many features of themore » modern fan system are readily apparent on the sonar mosaic. The 1.5 to 0.5-km (5000 to 1600-ft) wide channels meander intensely across the fan with sinuosities up to 2.5. Because of these meanders, the channel gradients decrease regularly across the fan despite changes in regional slope. Other channel-related targets include cutoff meanders, overbank deposits (especially small debris flows), and channel branchings. Other debris flows cover large areas of the fan and override channel/levee systems. Air-gun records show that this fan is built of a series of channel/levee systems that overlay one another. Channels from at least 6 of these systems are visible at the surface now, but apparently only one channel at a time has been active. The length of time needed to build a single channel/levee system is not known, but it appears to be rapid.« less

Polymer in a pore: Effect of confinement on the free energy barrier

NASA Astrophysics Data System (ADS)

Kumar, Sanjiv; Kumar, Sanjay

2018-06-01

We investigate the transfer of a polymer chain from cis- side to trans- side through two types of pores: cone-shaped channel and flat-channel. Using the exact enumeration technique, we obtain the free energy landscapes of a polymer chain for such systems. We have also calculated the free-energy barrier of a polymer chain attached to the edge of the pore. The model system allows us to calculate the force required to pull polymer from the pore and stall-force to confine polymer within the pore.

Creation of backdoors in quantum communications via laser damage

NASA Astrophysics Data System (ADS)

Makarov, Vadim; Bourgoin, Jean-Philippe; Chaiwongkhot, Poompong; Gagné, Mathieu; Jennewein, Thomas; Kaiser, Sarah; Kashyap, Raman; Legré, Matthieu; Minshull, Carter; Sajeed, Shihan

2016-09-01

Practical quantum communication (QC) protocols are assumed to be secure provided implemented devices are properly characterized and all known side channels are closed. We show that this is not always true. We demonstrate a laser-damage attack capable of modifying device behavior on demand. We test it on two practical QC systems for key distribution and coin tossing, and show that newly created deviations lead to side channels. This reveals that laser damage is a potential security risk to existing QC systems, and necessitates their testing to guarantee security.

Flow structure, heat transfer and pressure drop in varying aspect ratio two-pass rectangular smooth channels

NASA Astrophysics Data System (ADS)

Siddique, Waseem; El-Gabry, Lamyaa; Shevchuk, Igor V.; Hushmandi, Narmin B.; Fransson, Torsten H.

2012-05-01

Two-pass channels are used for internal cooling in a number of engineering systems e.g., gas turbines. Fluid travelling through the curved path, experiences pressure and centrifugal forces, that result in pressure driven secondary motion. This motion helps in moving the cold high momentum fluid from the channel core to the side walls and plays a significant role in the heat transfer in the channel bend and outlet pass. The present study investigates using Computational Fluid Dynamics (CFD), the flow structure, heat transfer enhancement and pressure drop in a smooth channel with varying aspect ratio channel at different divider-to-tip wall distances. Numerical simulations are performed in two-pass smooth channel with aspect ratio Win/H = 1:3 at inlet pass and Wout/H = 1:1 at outlet pass for a variety of divider-to-tip wall distances. The results show that with a decrease in aspect ratio of inlet pass of the channel, pressure loss decreases. The divider-to-tip wall distance (Wel) not only influences the pressure drop, but also the heat transfer enhancement at the bend and outlet pass. With an increase in the divider-to-tip wall distance, the areas of enhanced heat transfer shifts from side walls of outlet pass towards the inlet pass. To compromise between heat transfer and pressure drop in the channel, Wel/H = 0.88 is found to be optimum for the channel under study.

Flow over a membrane-covered, fluid-filled cavity.

PubMed

Thomson, Scott L; Mongeau, Luc; Frankel, Steven H

2007-01-01

The flow-induced response of a membrane covering a fluid-filled cavity located in a section of a rigid-walled channel was explored using finite element analysis. The membrane was initially aligned with the channel wall and separated the channel fluid from the cavity fluid. As fluid flowed over the membrane-covered cavity, a streamwise-dependent transmural pressure gradient caused membrane deformation. This model has application to synthetic models of the vocal fold cover layer used in voice production research. In this paper, the model is introduced and responses of the channel flow, the membrane, and the cavity flow are summarized for a range of flow and membrane parameters. It is shown that for high values of cavity fluid viscosity, the intracavity pressure and the beam deflection both reached steady values. For combinations of low cavity viscosity and sufficiently large upstream pressures, large-amplitude membrane vibrations resulted. Asymmetric conditions were introduced by creating cavities on opposing sides of the channel and assigning different stiffness values to the two membranes. The asymmetry resulted in reduction in or cessation of vibration amplitude, depending on the degree of asymmetry, and in significant skewing of the downstream flow field.

Spirit Studies Rock Outcrop at Home Plate

NASA Image and Video Library

2006-03-06

This image shows two flat-topped, layered rocks with angular edges almost side by side, except they are separated by a smaller rock and two thin channels of reddish-brown sand. The bare rock surfaces are a light blue-gray

Restoration of recto-verso colour documents using correlated component analysis

NASA Astrophysics Data System (ADS)

Tonazzini, Anna; Bedini, Luigi

2013-12-01

In this article, we consider the problem of removing see-through interferences from pairs of recto-verso documents acquired either in grayscale or RGB modality. The see-through effect is a typical degradation of historical and archival documents or manuscripts, and is caused by transparency or seeping of ink from the reverse side of the page. We formulate the problem as one of separating two individual texts, overlapped in the recto and verso maps of the colour channels through a linear convolutional mixing operator, where the mixing coefficients are unknown, while the blur kernels are assumed known a priori or estimated off-line. We exploit statistical techniques of blind source separation to estimate both the unknown model parameters and the ideal, uncorrupted images of the two document sides. We show that recently proposed correlated component analysis techniques overcome the already satisfactory performance of independent component analysis techniques and colour decorrelation, when the two texts are even sensibly correlated.

Diode-pumped laser with improved pumping system

DOEpatents

Chang, Jim J.

2004-03-09

A laser wherein pump radiation from laser diodes is delivered to a pump chamber and into the lasing medium by quasi-three-dimensional compound parabolic concentrator light channels. The light channels have reflective side walls with a curved surface and reflective end walls with a curved surface. A flow tube between the lasing medium and the light channel has a roughened surface.

Regulation of voltage-gated potassium channels attenuates resistance of side-population cells to gefitinib in the human lung cancer cell line NCI-H460.

PubMed

Choi, Seon Young; Kim, Hang-Rae; Ryu, Pan Dong; Lee, So Yeong

2017-02-21

Side-population (SP) cells that exclude anti-cancer drugs have been found in various tumor cell lines. Moreover, SP cells have a higher proliferative potential and drug resistance than main population cells (Non-SP cells). Also, several ion channels are responsible for the drug resistance and proliferation of SP cells in cancer. To confirm the expression and function of voltage-gated potassium (Kv) channels of SP cells, these cells, as well as highly expressed ATP-binding cassette (ABC) transporters and stemness genes, were isolated from a gefitinib-resistant human lung adenocarcinoma cell line (NCI-H460), using Hoechst 33342 efflux. In the present study, we found that mRNA expression of Kv channels in SP cells was different compared to Non-SP cells, and the resistance of SP cells to gefitinib was weakened with a combination treatment of gefitinib and Kv channel blockers or a Kv7 opener, compared to single-treatment gefitinib, through inhibition of the Ras-Raf signaling pathway. The findings indicate that Kv channels in SP cells could be new targets for reducing the resistance to gefitinib.

Extracellular blockade of K(+) channels by TEA: results from molecular dynamics simulations of the KcsA channel.

PubMed

Crouzy, S; Bernèche, S; Roux, B

2001-08-01

TEA is a classical blocker of K(+) channels. From mutagenesis studies, it has been shown that external blockade by TEA is strongly dependent upon the presence of aromatic residue at Shaker position 449 which is located near the extracellular entrance to the pore (Heginbotham, L., and R. MacKinnon. 1992. Neuron. 8:483-491). The data suggest that TEA interacts simultaneously with the aromatic residues of the four monomers. The determination of the 3-D structure of the KcsA channel using X-ray crystallography (Doyle, D.A., J.M. Cabral, R.A. Pfuetzner, A. Kuo, J.M. Gulbis, S.L. Cohen, B.T. Chait, and R. MacKinnon. 1998. Science. 280:69-77) has raised some issues that remain currently unresolved concerning the interpretation of these observations. In particular, the center of the Tyr82 side chains in KcsA (corresponding to position 449 in Shaker) forms a square of 11.8-A side, a distance which is too large to allow simultaneous interactions of a TEA molecule with the four aromatic side chains. In this paper, the external blockade by TEA is explored by molecular dynamics simulations of an atomic model of KcsA in an explicit phospholipid bilayer with aqueous salt solution. It is observed, in qualitative accord with the experimental results, that TEA is stable when bound to the external side of the wild-type KcsA channel (with Tyr82), but is unstable when bound to a mutant channel in which the tyrosine residue has been substituted by a threonine. The free energy profile of TEA relative to the pore is calculated using umbrella sampling simulations to characterize quantitatively the extracellular blockade. It is found, in remarkable agreement with the experiment, that the TEA is more stably bound by 2.3 kcal/mol to the channel with four tyrosine residues. In the case of the wild-type KcsA channel, TEA (which has the shape of a flattened oblate spheroid) acts as an ideal plug blocking the pore. In contrast, it is considerably more off-centered and tilted in the case of the mutant channel. The enhanced stability conferred by the tyrosine residues does not arise from Pi-cation interactions, but appears to be due to differences in the hydration structure of the TEA. Finally, it is shown that the experimentally observed voltage dependence of TEA block, which is traditionally interpreted in terms of the physical position of the TEA along the axis of the pore, must arise indirectly via coupling with the ions in the pore.

Bar dynamics and channel junctions in scale-experiments of estuaries

NASA Astrophysics Data System (ADS)

Leuven, J.; Braat, L.; van Dijk, W. M.; Haas, T. D.; Kleinhans, M. G.

2017-12-01

The evolution of channels and bars in estuaries has high socio-economic relevance, with strong implications for navigation, dredging and ecology. However, the spatial and temporal evolution of channels and bars in estuaries is poorly understood. Here, we study feedbacks of bar morphodynamics on widening and narrowing of estuaries. Therefore, we conducted an experiment in a 20 m long and 3 m wide tilting flume (the 'Metronome'), in which we monitored the evolution of a self-formed estuary that developed from an intial straight channel into an irregular planform with multiple channels, braided bars and a meandering ebb channel. At locations where the estuary width is confined, major channel junctions occur, while the zones between the junctions are characterised by high braiding indices, periodically migrating channels and a relatively large estuary width. The junction locations were forced by the in- and outflow locations on the sides of the ebb-tidal delta and at the location where the channel pattern transitions from multiple channels into a single channel. In the middle of the estuary, self-confinement occurred by sedimentation on the sides of the estuary, which caused another major junction. The channel orientation at the junctions steers the morphodynamics of channels and bars immediately landward and seaward, because the orientation of inflow from the ebb-tidal delta and landward river perpetually varies. In natural systems major junction locations are mostly forced by inherited geology or human engineering. However, this study concludes that even without external forcing, the estuary planform will not converge to an ideal shape but will self-confine at major junctions and widens in the adjacent zones, resulting in an irregular planform shape.

Channel Networks on Large Fans: Refining Analogs for the Ridge-forming Unit, Sinus Meridiani

NASA Technical Reports Server (NTRS)

Wilkinson, Justin

2009-01-01

Stream channels are generally thought of as forming within confined valley settings, separated by interfluves. Sinuous ridges on Mars and Earth are often interpreted as stream channels inverted by subsequent erosion of valley sides. In the case of the ridge-forming unit (RFU), this interpretation fails to explain the (i) close spacing of the ridges, which are (ii) organized in networks, and which (iii) cover large areas (approximately 175,000 km (exp 2)). Channel networks on terrestrial fans develop unconfined by valley slopes. Large fans (100s km long) are low-angle, fluvial features, documented worldwide, with characteristics that address these aspects of the RFU. Ridge patterns Channels on large fans provide an analog for the sinuous and elongated morphology of RFU ridges, but more especially for other patterns such as subparallel, branching and crossing networks. Branches are related to splays (delta-like distributaries are rare), whose channels can rejoin the main channel. Crossing patterns can be caused by even slight sinuosity splay-related side channels often intersect. An avulsion node distant from the fan apex, gives rise to channels with slightly different, and hence intersecting, orientations. Channels on neighboring fans intersect along the common fan margin. 2. Network density Channels are the dominant feature on large terrestrial fans (lakes and dune fields are minor). Inverted landscapes on subsequently eroded fans thus display indurated channels as networks of significantly close-spaced ridges. 3. Channel networks covering large areas Areas of individual large terrestrial fans can reach >200,000 km 2 (105-6 km 2 with nested fans), providing an analog for the wide area distribution of the RFU.

Selective blockade of TRPA1 channel attenuates pathological pain without altering noxious cold sensation or body temperature regulation.

PubMed

Chen, Jun; Joshi, Shailen K; DiDomenico, Stanley; Perner, Richard J; Mikusa, Joe P; Gauvin, Donna M; Segreti, Jason A; Han, Ping; Zhang, Xu-Feng; Niforatos, Wende; Bianchi, Bruce R; Baker, Scott J; Zhong, Chengmin; Simler, Gricelda H; McDonald, Heath A; Schmidt, Robert G; McGaraughty, Steve P; Chu, Katharine L; Faltynek, Connie R; Kort, Michael E; Reilly, Regina M; Kym, Philip R

2011-05-01

Despite the increasing interest in TRPA1 channel as a pain target, its role in cold sensation and body temperature regulation is not clear; the efficacy and particularly side effects resulting from channel blockade remain poorly understood. Here we use a potent, selective, and bioavailable antagonist to address these issues. A-967079 potently blocks human (IC(50): 51 nmol/L, electrophysiology, 67 nmol/L, Ca(2+) assay) and rat TRPA1 (IC(50): 101 nmol/L, electrophysiology, 289 nmol/L, Ca(2+) assay). It is >1000-fold selective over other TRP channels, and is >150-fold selective over 75 other ion channels, enzymes, and G-protein-coupled receptors. Oral dosing of A-967079 produces robust drug exposure in rodents, and exhibits analgesic efficacy in allyl isothiocyanate-induced nocifensive response and osteoarthritic pain in rats (ED(50): 23.2 mg/kg, p.o.). A-967079 attenuates cold allodynia produced by nerve injury but does not alter noxious cold sensation in naive animals, suggesting distinct roles of TRPA1 in physiological and pathological states. Unlike TRPV1 antagonists, A-967079 does not alter body temperature. It also does not produce locomotor or cardiovascular side effects. Collectively, these data provide novel insights into TRPA1 function and suggest that the selective TRPA1 blockade may present a viable strategy for alleviating pain without untoward side effects. Copyright © 2011 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

Gating the glutamate gate of CLC-2 chloride channel by pore occupancy

PubMed Central

De Jesús-Pérez, José J.; Castro-Chong, Alejandra; Shieh, Ru-Chi; Hernández-Carballo, Carmen Y.; De Santiago-Castillo, José A.

2016-01-01

CLC-2 channels are dimeric double-barreled chloride channels that open in response to hyperpolarization. Hyperpolarization activates protopore gates that independently regulate the permeability of the pore in each subunit and the common gate that affects the permeability through both pores. CLC-2 channels lack classic transmembrane voltage–sensing domains; instead, their protopore gates (residing within the pore and each formed by the side chain of a glutamate residue) open under repulsion by permeant intracellular anions or protonation by extracellular H+. Here, we show that voltage-dependent gating of CLC-2: (a) is facilitated when permeant anions (Cl−, Br−, SCN−, and I−) are present in the cytosolic side; (b) happens with poorly permeant anions fluoride, glutamate, gluconate, and methanesulfonate present in the cytosolic side; (c) depends on pore occupancy by permeant and poorly permeant anions; (d) is strongly facilitated by multi-ion occupancy; (e) is absent under likely protonation conditions (pHe = 5.5 or 6.5) in cells dialyzed with acetate (an impermeant anion); and (f) was the same at intracellular pH 7.3 and 4.2; and (g) is observed in both whole-cell and inside-out patches exposed to increasing [Cl−]i under unlikely protonation conditions (pHe = 10). Thus, based on our results we propose that hyperpolarization activates CLC-2 mainly by driving intracellular anions into the channel pores, and that protonation by extracellular H+ plays a minor role in dislodging the glutamate gate. PMID:26666914

Parameter-dependent behaviour of periodic channels in a locus of boundary crisis

NASA Astrophysics Data System (ADS)

Rankin, James; Osinga, Hinke M.

2017-06-01

A boundary crisis occurs when a chaotic attractor outgrows its basin of attraction and suddenly disappears. As previously reported, the locus of a boundary crisis is organised by homo- or heteroclinic tangencies between the stable and unstable manifolds of saddle periodic orbits. In two parameters, such tangencies lead to curves, but the locus of boundary crisis along those curves exhibits gaps or channels, in which other non-chaotic attractors persist. These attractors are stable periodic orbits which themselves can undergo a cascade of period-doubling bifurcations culminating in multi-component chaotic attractors. The canonical diffeomorphic two-dimensional Hénon map exhibits such periodic channels, which are structured in a particular ordered way: each channel is bounded on one side by a saddle-node bifurcation and on the other by a period-doubling cascade to chaos; furthermore, all channels seem to have the same orientation, with the saddle-node bifurcation always on the same side. We investigate the locus of boundary crisis in the Ikeda map, which models the dynamics of energy levels in a laser ring cavity. We find that the Ikeda map features periodic channels with a richer and more general organisation than for the Hénon map. Using numerical continuation, we investigate how the periodic channels depend on a third parameter and characterise how they split into multiple channels with different properties.

Passive injection control for microfluidic systems

DOEpatents

Paul, Phillip H.; Arnold, Don W.; Neyer, David W.

2004-12-21

Apparatus for eliminating siphoning, "dead" regions, and fluid concentration gradients in microscale analytical devices. In its most basic embodiment, the present invention affords passive injection control for both electric field-driven and pressure-driven systems by providing additional fluid flow channels or auxiliary channels disposed on either side of a sample separation column. The auxiliary channels are sized such that volumetric fluid flow rate through these channels, while sufficient to move the sample away from the sample injection region in a timely fashion, is less than that through the sample separation channel or chromatograph.

Toward Exposing Timing-Based Probing Attacks in Web Applications †

PubMed Central

Mao, Jian; Chen, Yue; Shi, Futian; Jia, Yaoqi; Liang, Zhenkai

2017-01-01

Web applications have become the foundation of many types of systems, ranging from cloud services to Internet of Things (IoT) systems. Due to the large amount of sensitive data processed by web applications, user privacy emerges as a major concern in web security. Existing protection mechanisms in modern browsers, e.g., the same origin policy, prevent the users’ browsing information on one website from being directly accessed by another website. However, web applications executed in the same browser share the same runtime environment. Such shared states provide side channels for malicious websites to indirectly figure out the information of other origins. Timing is a classic side channel and the root cause of many recent attacks, which rely on the variations in the time taken by the systems to process different inputs. In this paper, we propose an approach to expose the timing-based probing attacks in web applications. It monitors the browser behaviors and identifies anomalous timing behaviors to detect browser probing attacks. We have prototyped our system in the Google Chrome browser and evaluated the effectiveness of our approach by using known probing techniques. We have applied our approach on a large number of top Alexa sites and reported the suspicious behavior patterns with corresponding analysis results. Our theoretical analysis illustrates that the effectiveness of the timing-based probing attacks is dramatically limited by our approach. PMID:28245610

Toward Exposing Timing-Based Probing Attacks in Web Applications.

PubMed

Mao, Jian; Chen, Yue; Shi, Futian; Jia, Yaoqi; Liang, Zhenkai

2017-02-25

Web applications have become the foundation of many types of systems, ranging from cloud services to Internet of Things (IoT) systems. Due to the large amount of sensitive data processed by web applications, user privacy emerges as a major concern in web security. Existing protection mechanisms in modern browsers, e.g., the same origin policy, prevent the users' browsing information on one website from being directly accessed by another website. However, web applications executed in the same browser share the same runtime environment. Such shared states provide side channels for malicious websites to indirectly figure out the information of other origins. Timing is a classic side channel and the root cause of many recent attacks, which rely on the variations in the time taken by the systems to process different inputs. In this paper, we propose an approach to expose the timing-based probing attacks in web applications. It monitors the browser behaviors and identifies anomalous timing behaviors to detect browser probing attacks. We have prototyped our system in the Google Chrome browser and evaluated the effectiveness of our approach by using known probing techniques. We have applied our approach on a large number of top Alexa sites and reported the suspicious behavior patterns with corresponding analysis results. Our theoretical analysis illustrates that the effectiveness of the timing-based probing attacks is dramatically limited by our approach.

A dimensional comparison between embedded 3D-printed and silicon microchannels

NASA Astrophysics Data System (ADS)

O'Connor, J.; Punch, J.; Jeffers, N.; Stafford, J.

2014-07-01

The subject of this paper is the dimensional characterization of embedded microchannel arrays created using contemporary 3D-printing fabrication techniques. Conventional microchannel arrays, fabricated using deep reactive ion etching techniques (DRIE) and wet-etching (KOH), are used as a benchmark for comparison. Rectangular and trapezoidal cross-sectional shapes were investigated. The channel arrays were 3D-printed in vertical and horizontal directions, to examine the influence of print orientation on channel characteristics. The 3D-printed channels were benchmarked against Silicon channels in terms of the following dimensional characteristics: cross-sectional area (CSA), perimeter, and surface profiles. The 3D-printed microchannel arrays demonstrated variances in CSA of 6.6-20% with the vertical printing approach yielding greater dimensional conformity than the horizontal approach. The measured CSA and perimeter of the vertical channels were smaller than the nominal dimensions, while the horizontal channels were larger in both CSA and perimeter due to additional side-wall roughness present throughout the channel length. This side-wall roughness caused significant shape distortion. Surface profile measurements revealed that the base wall roughness was approximately the resolution of current 3D-printers. A spatial periodicity was found along the channel length which appeared at different frequencies for each channel array. This paper concludes that vertical 3D-printing is superior to the horizontal printing approach, in terms of both dimensional fidelity and shape conformity and can be applied in microfluidic device applications.

Reconfigurable high-speed optical fibre networks: Optical wavelength conversion and switching using VCSELs to eliminate channel collisions

NASA Astrophysics Data System (ADS)

Boiyo, Duncan Kiboi; Chabata, T. V.; Kipnoo, E. K. Rotich; Gamatham, R. R. G.; Leitch, A. W. R.; Gibbon, T. B.

2017-01-01

We experimentally provide an alternative solution to channel collisions through up-wavelength conversion and switching by using vertical cavity surface-emitting lasers (VCSELs). This has been achieved by utilizing purely optical wavelength conversion on VCSELs at the low attenuation, 1550 nm transmission window. The corresponding transmission and bit error-rate (BER) performance evaluation is also presented. In this paper, two 1550 nm VCSELs with 50-150 GHz channel spacing are modulated with a 10 Gb/s NRZ PRBS 27-1 data and their interferences investigated. A channel interference penalty range of 0.15-1.63 dB is incurred for 150-50 GHz channel spacing without transmission. To avoid channel collisions and to minimize high interference penalties, the transmitting VCSEL with data is injected into the side-mode of a slave VCSEL to obtain a new up converted wavelength. A 16 dB extinction ratio of the incoming wavelength is achieved when a 15 dBm transmitting beam is injected into the side-mode of a -4.5 dBm slave VCSEL. At 8.5 Gb/s, a 1.1 dB conversion and a 0.5 dB transmission penalties are realized when the converted wavelength is transmitted over a 24.7 km G.655 fibre. This work offers a low-cost, effective wavelength conversion and channel switching to reduce channel collision probability by reconfiguring channels at the node of networks.

Inertio-elastic mixing in a straight microchannel with side wells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hong, Sun Ok; Cooper-White, Justin J.; School of Chemical Engineering, University of Queensland, St Lucia, 4072 QLD

Mixing remains a challenging task in microfluidic channels because of their inherently small length scale. In this work, we propose an efficient microfluidic mixer based on the chaotic vortex dynamics of a viscoelastic flow in a straight channel with side wells. When the inertia and elasticity of a dilute polymer solution are balanced (i.e., the Reynolds number Re and Weissenberg number Wi are both on the order of 10{sup 1}), chaotic vortices appear in the side wells (inertio-elastic flow instability), enhancing the mixing of adjacent fluid streams. However, there is no chaotic vortex motion in Newtonian flows for any flowmore » rate. Efficient mixing by such an inertio-elastic instability is found to be relevant for a wide range of Re values.« less

Observed side feeding in incomplete fusion dynamics in 16O + 160Gd reaction at energy ∼5.6 MeV/A: Spin distribution measurements

NASA Astrophysics Data System (ADS)

Ali, Rahbar; Afzal Ansari, M.; Singh, D.; Kumar, Rakesh; Singh, D. P.; Sharma, M. K.; Gupta, Unnati; Singh, B. P.; Shidling, P. D.; Negi, Dinesh; Muralithar, S.; Singh, R. P.; Bhowmik, R. K.

2017-12-01

Spin distributions of various residues populated via complete fusion (CF) and incomplete fusion (ICF) reactions in the interaction of 16O with 160Gd at the projectile energy Eproj ∼ 5.6 MeV/A have been studied. The experimentally measured spin distributions of the residues associated with the ICF reactions are found to be distinctly different from those populated via the CF reactions. An attempt has been made to extract the side-feeding pattern from the spin distributions of CF and ICF reaction products. It has been observed that the CF products are strongly fed over a broad spin range. But, no side-feeding takes place in the low observed spins as low partial waves are strongly hindered in the fast α-emission channels (associated with ICF) in the forward direction. It has also been observed that the mean input angular momentum for direct α-emitting (ICF) channels is relatively higher than evaporation α-emitting (CF) channels, and it increases with direct α-multiplicity in forward direction.

A security-awareness virtual machine management scheme based on Chinese wall policy in cloud computing.

PubMed

Yu, Si; Gui, Xiaolin; Lin, Jiancai; Tian, Feng; Zhao, Jianqiang; Dai, Min

2014-01-01

Cloud computing gets increasing attention for its capacity to leverage developers from infrastructure management tasks. However, recent works reveal that side channel attacks can lead to privacy leakage in the cloud. Enhancing isolation between users is an effective solution to eliminate the attack. In this paper, to eliminate side channel attacks, we investigate the isolation enhancement scheme from the aspect of virtual machine (VM) management. The security-awareness VMs management scheme (SVMS), a VMs isolation enhancement scheme to defend against side channel attacks, is proposed. First, we use the aggressive conflict of interest relation (ACIR) and aggressive in ally with relation (AIAR) to describe user constraint relations. Second, based on the Chinese wall policy, we put forward four isolation rules. Third, the VMs placement and migration algorithms are designed to enforce VMs isolation between the conflict users. Finally, based on the normal distribution, we conduct a series of experiments to evaluate SVMS. The experimental results show that SVMS is efficient in guaranteeing isolation between VMs owned by conflict users, while the resource utilization rate decreases but not by much.

Microphase separation and the formation of ion conductivity channels in poly(ionic liquid)s: A coarse-grained molecular dynamics study

NASA Astrophysics Data System (ADS)

Weyman, Alexander; Bier, Markus; Holm, Christian; Smiatek, Jens

2018-05-01

We study generic properties of poly(ionic liquid)s (PILs) via coarse-grained molecular dynamics simulations in bulk solution and under confinement. The influence of different side chain lengths on the spatial properties of the PIL systems and on the ionic transport mechanism is investigated in detail. Our results reveal the formation of apolar and polar nanodomains with increasing side chain length in good agreement with previous results for molecular ionic liquids. The ion transport numbers are unaffected by the occurrence of these domains, and the corresponding values highlight the potential role of PILs as single-ion conductors in electrochemical devices. In contrast to bulk behavior, a pronounced formation of ion conductivity channels in confined systems is initiated in close vicinity to the boundaries. We observe higher ion conductivities in these channels for increasing PIL side chain lengths in comparison with bulk values and provide an explanation for this effect. The appearance of these domains points to an improved application of PILs in modern polymer electrolyte batteries.

Tidal truncation and barotropic convergence in a channel network tidally driven from opposing entrances

USGS Publications Warehouse

Warner, J.C.; Schoellhamer, D.; Schladow, G.

2003-01-01

Residual circulation patterns in a channel network that is tidally driven from entrances on opposite sides are controlled by the temporal phasing and spatial asymmetry of the two forcing tides. The Napa/Sonoma Marsh Complex in San Francisco Bay, CA, is such a system. A sill on the west entrance to the system prevents a complete tidal range at spring tides that results in tidal truncation of water levels. Tidal truncation does not occur on the east side but asymmetries develop due to friction and off-channel wetland storage. The east and west asymmetric tides meet in the middle to produce a barotropic convergence zone that controls the transport of water and sediment. During spring tides, tidally averaged water-surface elevations are higher on the truncated west side. This creates tidally averaged fluxes of water and sediment to the east. During neap tides, the water levels are not truncated and the propagation speed of the tides controls residual circulation, creating a tidally averaged flux in the opposite direction. ?? 2003 Elsevier Science B.V. All rights reserved.

A Security-Awareness Virtual Machine Management Scheme Based on Chinese Wall Policy in Cloud Computing

PubMed Central

Gui, Xiaolin; Lin, Jiancai; Tian, Feng; Zhao, Jianqiang; Dai, Min

2014-01-01

Cloud computing gets increasing attention for its capacity to leverage developers from infrastructure management tasks. However, recent works reveal that side channel attacks can lead to privacy leakage in the cloud. Enhancing isolation between users is an effective solution to eliminate the attack. In this paper, to eliminate side channel attacks, we investigate the isolation enhancement scheme from the aspect of virtual machine (VM) management. The security-awareness VMs management scheme (SVMS), a VMs isolation enhancement scheme to defend against side channel attacks, is proposed. First, we use the aggressive conflict of interest relation (ACIR) and aggressive in ally with relation (AIAR) to describe user constraint relations. Second, based on the Chinese wall policy, we put forward four isolation rules. Third, the VMs placement and migration algorithms are designed to enforce VMs isolation between the conflict users. Finally, based on the normal distribution, we conduct a series of experiments to evaluate SVMS. The experimental results show that SVMS is efficient in guaranteeing isolation between VMs owned by conflict users, while the resource utilization rate decreases but not by much. PMID:24688434

Amyloplast sedimentation dynamics in maize columella cells support a new model for the gravity-sensing apparatus of roots

NASA Technical Reports Server (NTRS)

Yoder, T. L.; Zheng, H. Q.; Todd, P.; Staehelin, L. A.

2001-01-01

Quantitative analysis of statolith sedimentation behavior was accomplished using videomicroscopy of living columella cells of corn (Zea mays) roots, which displayed no systematic cytoplasmic streaming. Following 90 degrees rotation of the root, the statoliths moved downward along the distal wall and then spread out along the bottom with an average velocity of 1.7 microm min(-1). When statolith trajectories traversed the complete width or length of the cell, they initially moved horizontally toward channel-initiation sites and then moved vertically through the channels to the lower side of the reoriented cell where they again dispersed. These statoliths exhibited a significantly lower average velocity than those sedimenting on distal-to-side trajectories. In addition, although statoliths undergoing distal-to-side sedimentation began at their highest velocity and slowed monotonically as they approached the lower cell membrane, statoliths crossing the cell's central region remained slow initially and accelerated to maximum speed once they reached a channel. The statoliths accelerated sooner, and the channeling effect was less pronounced in roots treated with cytochalasin D. Parallel ultrastructural studies of high-pressure frozen-freeze-substituted columella cells suggest that the low-resistance statolith pathway in the cell periphery corresponds to the sharp interface between the endoplasmic reticulum (ER)-rich cortical and the ER-devoid central region of these cells. The central region is also shown to contain an actin-based cytoskeletal network in which the individual, straight, actin-like filaments are randomly distributed. To explain these findings as well as the results of physical simulation experiments, we have formulated a new, tensegrity-based model of gravity sensing in columella cells. This model envisages the cytoplasm as pervaded by an actin-based cytoskeletal network that is denser in the ER-devoid central region than in the ER-rich cell cortex and is linked to stretch receptors in the plasma membrane. Sedimenting statoliths are postulated to produce a directional signal by locally disrupting the network and thereby altering the balance of forces acting on the receptors in different plasma membrane regions.

Amyloplast Sedimentation Dynamics in Maize Columella Cells Support a New Model for the Gravity-Sensing Apparatus of Roots1

PubMed Central

Yoder, Thomas L.; Zheng, Hui-qiong; Todd, Paul; Staehelin, L. Andrew

2001-01-01

Quantitative analysis of statolith sedimentation behavior was accomplished using videomicroscopy of living columella cells of corn (Zea mays) roots, which displayed no systematic cytoplasmic streaming. Following 90° rotation of the root, the statoliths moved downward along the distal wall and then spread out along the bottom with an average velocity of 1.7 μm min−1. When statolith trajectories traversed the complete width or length of the cell, they initially moved horizontally toward channel-initiation sites and then moved vertically through the channels to the lower side of the reoriented cell where they again dispersed. These statoliths exhibited a significantly lower average velocity than those sedimenting on distal-to-side trajectories. In addition, although statoliths undergoing distal-to-side sedimentation began at their highest velocity and slowed monotonically as they approached the lower cell membrane, statoliths crossing the cell's central region remained slow initially and accelerated to maximum speed once they reached a channel. The statoliths accelerated sooner, and the channeling effect was less pronounced in roots treated with cytochalasin D. Parallel ultrastructural studies of high-pressure frozen-freeze-substituted columella cells suggest that the low-resistance statolith pathway in the cell periphery corresponds to the sharp interface between the endoplasmic reticulum (ER)-rich cortical and the ER-devoid central region of these cells. The central region is also shown to contain an actin-based cytoskeletal network in which the individual, straight, actin-like filaments are randomly distributed. To explain these findings as well as the results of physical simulation experiments, we have formulated a new, tensegrity-based model of gravity sensing in columella cells. This model envisages the cytoplasm as pervaded by an actin-based cytoskeletal network that is denser in the ER-devoid central region than in the ER-rich cell cortex and is linked to stretch receptors in the plasma membrane. Sedimenting statoliths are postulated to produce a directional signal by locally disrupting the network and thereby altering the balance of forces acting on the receptors in different plasma membrane regions. PMID:11161060

Amyloplast sedimentation dynamics in maize columella cells support a new model for the gravity-sensing apparatus of roots.

PubMed

Yoder, T L; Zheng, H Q; Todd, P; Staehelin, L A

2001-02-01

Quantitative analysis of statolith sedimentation behavior was accomplished using videomicroscopy of living columella cells of corn (Zea mays) roots, which displayed no systematic cytoplasmic streaming. Following 90 degrees rotation of the root, the statoliths moved downward along the distal wall and then spread out along the bottom with an average velocity of 1.7 microm min(-1). When statolith trajectories traversed the complete width or length of the cell, they initially moved horizontally toward channel-initiation sites and then moved vertically through the channels to the lower side of the reoriented cell where they again dispersed. These statoliths exhibited a significantly lower average velocity than those sedimenting on distal-to-side trajectories. In addition, although statoliths undergoing distal-to-side sedimentation began at their highest velocity and slowed monotonically as they approached the lower cell membrane, statoliths crossing the cell's central region remained slow initially and accelerated to maximum speed once they reached a channel. The statoliths accelerated sooner, and the channeling effect was less pronounced in roots treated with cytochalasin D. Parallel ultrastructural studies of high-pressure frozen-freeze-substituted columella cells suggest that the low-resistance statolith pathway in the cell periphery corresponds to the sharp interface between the endoplasmic reticulum (ER)-rich cortical and the ER-devoid central region of these cells. The central region is also shown to contain an actin-based cytoskeletal network in which the individual, straight, actin-like filaments are randomly distributed. To explain these findings as well as the results of physical simulation experiments, we have formulated a new, tensegrity-based model of gravity sensing in columella cells. This model envisages the cytoplasm as pervaded by an actin-based cytoskeletal network that is denser in the ER-devoid central region than in the ER-rich cell cortex and is linked to stretch receptors in the plasma membrane. Sedimenting statoliths are postulated to produce a directional signal by locally disrupting the network and thereby altering the balance of forces acting on the receptors in different plasma membrane regions.

Chemical construction and structural permutation of neurotoxic natural product, antillatoxin: importance of the three-dimensional structure of the bulky side chain

PubMed Central

INOUE, Masayuki

2014-01-01

Antillatoxin 1 is a unique natural product that displays potent neurotoxic and neuritogenic activities through activation of voltage-gated sodium channels. The peptidic macrocycle of 1 was attached to a side chain with an exceptionally high degree of methylation. In this review, we discuss the total synthesis and biological evaluation of 1 and its analogues. First we describe an efficient synthetic route to 1. This strategy enabled the unified preparation of nine side chain analogues. Structure-activity relationship studies of these analogues revealed that subtle side chain modification leads to dramatic changes in activity, and detailed structural analyses indicated the importance of the overall size and three dimensional shape of the side chain. Based on these data, we designed and synthesized a photoresponsive analogue, proving that the activity of 1 was modulated via a photochemical reaction. The knowledge accumulated through these studies will be useful for the rational design of new tailor-made molecules to control the function and behavior of ion channels. PMID:24522155

Resolving the negative potential side (n-side) water-accessible proton pathway of F-type ATP synthase by molecular dynamics simulations.

PubMed

Gohlke, Holger; Schlieper, Daniel; Groth, Georg

2012-10-19

The rotation of F(1)F(o)-ATP synthase is powered by the proton motive force across the energy-transducing membrane. The protein complex functions like a turbine; the proton flow drives the rotation of the c-ring of the transmembrane F(o) domain, which is coupled to the ATP-producing F(1) domain. The hairpin-structured c-protomers transport the protons by reversible protonation/deprotonation of a conserved Asp/Glu at the outer transmembrane helix (TMH). An open question is the proton transfer pathway through the membrane at atomic resolution. The protons are thought to be transferred via two half-channels to and from the conserved cAsp/Glu in the middle of the membrane. By molecular dynamics simulations of c-ring structures in a lipid bilayer, we mapped a water channel as one of the half-channels. We also analyzed the suppressor mutant cP24D/E61G in which the functional carboxylate is shifted to the inner TMH of the c-protomers. Current models concentrating on the "locked" and "open" conformations of the conserved carboxylate side chain are unable to explain the molecular function of this mutant. Our molecular dynamics simulations revealed an extended water channel with additional water molecules bridging the distance of the outer to the inner TMH. We suggest that the geometry of the water channel is an important feature for the molecular function of the membrane part of F(1)F(o)-ATP synthase. The inclination of the proton pathway isolates the two half-channels and may contribute to a favorable clockwise rotation in ATP synthesis mode.

Importance of physical and hydraulic characteristics to unionid mussels: A retrospective analysis in a reach of large river

USGS Publications Warehouse

Zigler, S.J.; Newton, T.J.; Steuer, J.J.; Bartsch, M.R.; Sauer, J.S.

2008-01-01

Interest in understanding physical and hydraulic factors that might drive distribution and abundance of freshwater mussels has been increasing due to their decline throughout North America. We assessed whether the spatial distribution of unionid mussels could be predicted from physical and hydraulic variables in a reach of the Upper Mississippi River. Classification and regression tree (CART) models were constructed using mussel data compiled from various sources and explanatory variables derived from GIS coverages. Prediction success of CART models for presence-absence of mussels ranged from 71 to 76% across three gears (brail, sled-dredge, and dive-quadrat) and 51% of the deviance in abundance. Models were largely driven by shear stress and substrate stability variables, but interactions with simple physical variables, especially slope, were also important. Geospatial models, which were based on tree model results, predicted few mussels in poorly connected backwater areas (e.g., floodplain lakes) and the navigation channel, whereas main channel border areas with high geomorphic complexity (e.g., river bends, islands, side channel entrances) and small side channels were typically favorable to mussels. Moreover, bootstrap aggregation of discharge-specific regression tree models of dive-quadrat data indicated that variables measured at low discharge were about 25% more predictive (PMSE = 14.8) than variables measured at median discharge (PMSE = 20.4) with high discharge (PMSE = 17.1) variables intermediate. This result suggests that episodic events such as droughts and floods were important in structuring mussel distributions. Although the substantial mussel and ancillary data in our study reach is unusual, our approach to develop exploratory statistical and geospatial models should be useful even when data are more limited. ?? 2007 Springer Science+Business Media B.V.

11. VIEW NORTH ALONG DOWNSTREAM BANK OF DAM FROM SOUTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. VIEW NORTH ALONG DOWNSTREAM BANK OF DAM FROM SOUTH SIDE OF CHANNEL ON DOWNSTREAM SIDE OF RESERVOIR - Upper Doughty Dam, 200 feet west of Garden State Parkway, 1.7 miles west of Absecon, Egg Harbor City, Atlantic County, NJ

23. View of Clark Fork Vehicle Bridge facing upwest side. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. View of Clark Fork Vehicle Bridge facing up-west side. Looking at structural connection of top chord, vertical laced channel and diagonal bars. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

The spatial arrangement of neritina virginea (gastropoda: neritidae) during upstream migration in a split-channel reach.

Treesearch

JUAN F. BLANCO; FREDERICK N. SCATENA

2007-01-01

This paper relates differences in flow hydraulics between a main channel (MC) and a side channel (SC) of a river to patterns of upstream migration by Neritina virginea (Neritidae: Gastropoda), a dominant diadromous snail in streams of Puerto Rico (Greater Antilles). Near-bed water velocity, snail density and shell size were measured on a weekly basis between August and...

Navigation Channel Improvement, Columbia River, Oregon and Washington - Oak Point to Longview Reach, River Miles 53 to 65. Hydraulic Model Investigation.

DTIC Science & Technology

1984-09-01

channel to Mount St. Helens (RM 87) and Rainier , Oregon, (RM 68), respectively. Side channels are located at Cathlamet and Longview. Pu s L 3. The...to 12 u : survey the movable bed consisted of a vertically graduated rod which was referenced to a portable horizontally graduated rail mounted on the

Neutronic reactor

DOEpatents

Lewis, Warren R.

1978-05-30

A graphite-moderated, water-cooled nuclear reactor including a plurality of rectangular graphite blocks stacked in abutting relationship in layers, alternate layers having axes which are normal to one another, alternate rows of blocks in alternate layers being provided with a channel extending through the blocks, said channeled blocks being provided with concave sides and having smaller vertical dimensions than adjacent blocks in the same layer, there being nuclear fuel in the channels.

Bedform migration in steep channels: from local avalanches to large scale changes

NASA Astrophysics Data System (ADS)

Mettra, F.; Heyman, J.; Ancey, C.

2013-12-01

Many studies have emphasized the strength of bedload transport fluctuations in steep streams, especially at low and intermediate transport conditions (relative to the threshold of incipient motion). The origins of these fluctuations, which appear on a wide range of time scales, are still not well understood. In this study, we present the data obtained from a 2D idealized laboratory experiment with the objective of simultaneously recording the channel bed evolution and bedload transport rate at a high temporal resolution. A 3-m long by 8-cm wide transparent flume filled with well-sorted natural gravel (d50=6.5 mm) was used. An efficient technique using accelerometers has been developed to record the arrival time of every particle at the outlet of the flume for long experimental durations (up to a few days). In addition, bed elevation was monitored using cameras filming from the side of the channel, allowing the observation of global aggradation/degradation as well as bedform migration. The experimental parameters were the water discharge, the flume inclination (from 2° to 5°) and the constant feeding rate of sediments. Large-scale bed evolution showed successive aggradation and rapid degradation periods. Indeed, the measured global channel slope, i.e. mean slope over the flume length, fluctuated continuously within a range sometimes wider than 1° (experimental parameters were constant over the entire run). The analysis of these fluctuations provides evidence that steep channels behave like metastable systems, similarly to grain piles. The metastable effects increased for steeper channels and lower transport conditions. In this measurement campaign, we mainly observed upstream-migrating antidunes. For each run, various antidune heights and celerities were measured. On average, the mean antidune migration rate increased with decreasing channel slope and increasing sediment feeding rate. Relatively rare tall and fast-moving antidunes appeared more frequently at high flume angles and produced intense solid discharge pulses. Moreover, small avalanches occurred on the steep lee sides of antidunes. From these results, we infer a mechanism of steep channel evolution. The time- and space-averaged profile of the bed in the streamwise direction depends on the experimental parameters. Variations in the profile result mainly from bedform migration. The instantaneous global state of the bed (which can be characterized by the global channel slope) controls the growth of bedforms, which can be seen as local instabilities. When the global channel slope approaches its critical value, local instabilities of higher amplitude can develop and create intense bedload transport pulses, leading to a less steep, but more stable bed profile.

Effects of Phenylalanine Substitutions in Gramicidin A on the Kinetics of Channel Formation in Vesicles and Channel Structure in SDS Micelles

PubMed Central

Jordan, J. B.; Easton, P. L.; Hinton, J. F.

2005-01-01

The common occurrence of Trp residues at the aqueous-lipid interface region of transmembrane channels is thought to be indicative of its importance for insertion and stabilization of the channel in membranes. To further investigate the effects of Trp→Phe substitution on the structure and function of the gramicidin channel, four analogs of gramicidin A have been synthesized in which the tryptophan residues at positions 9, 11, 13, and 15 are sequentially replaced with phenylalanine. The three-dimensional structure of each viable analog has been determined using a combination of two-dimensional NMR techniques and distance geometry-simulated annealing structure calculations. These phenylalanine analogs adopt a homodimer motif, consisting of two β6.3 helices joined by six hydrogen bonds at their NH2-termini. The replacement of the tryptophan residues does not have a significant effect on the backbone structure of the channels when compared to native gramicidin A, and only small effects are seen on side-chain conformations. Single-channel conductance measurements have shown that the conductance and lifetime of the channels are significantly affected by the replacement of the tryptophan residues (Wallace, 2000; Becker et al., 1991). The variation in conductance appears to be caused by the sequential removal of a tryptophan dipole, thereby removing the ion-dipole interaction at the channel entrance and at the ion binding site. Channel lifetime variations appear to be related to changing side chain-lipid interactions. This is supported by data relating to transport and incorporation kinetics. PMID:15501932

Effects of phenylalanine substitutions in gramicidin A on the kinetics of channel formation in vesicles and channel structure in SDS micelles.

PubMed

Jordan, J B; Easton, P L; Hinton, J F

2005-01-01

The common occurrence of Trp residues at the aqueous-lipid interface region of transmembrane channels is thought to be indicative of its importance for insertion and stabilization of the channel in membranes. To further investigate the effects of Trp-->Phe substitution on the structure and function of the gramicidin channel, four analogs of gramicidin A have been synthesized in which the tryptophan residues at positions 9, 11, 13, and 15 are sequentially replaced with phenylalanine. The three-dimensional structure of each viable analog has been determined using a combination of two-dimensional NMR techniques and distance geometry-simulated annealing structure calculations. These phenylalanine analogs adopt a homodimer motif, consisting of two beta6.3 helices joined by six hydrogen bonds at their NH2-termini. The replacement of the tryptophan residues does not have a significant effect on the backbone structure of the channels when compared to native gramicidin A, and only small effects are seen on side-chain conformations. Single-channel conductance measurements have shown that the conductance and lifetime of the channels are significantly affected by the replacement of the tryptophan residues (Wallace, 2000; Becker et al., 1991). The variation in conductance appears to be caused by the sequential removal of a tryptophan dipole, thereby removing the ion-dipole interaction at the channel entrance and at the ion binding site. Channel lifetime variations appear to be related to changing side chain-lipid interactions. This is supported by data relating to transport and incorporation kinetics.

Channel One: Asking the Wrong Questions.

ERIC Educational Resources Information Center

Rank, Hugh

1994-01-01

All arguments about the 10 minutes of Channel One programming are side issues, including concerns about the bias or superficiality of new coverage, "infotainment" methods, and learning effectiveness. The main issue is the presence of television advertising (commercial persuasion)--aimed at a captive audience of schoolchildren and…

Predicting NonInertial Effects with Algebraic Stress Models which Account for Dissipation Rate Anisotropies

NASA Technical Reports Server (NTRS)

Jongen, T.; Machiels, L.; Gatski, T. B.

1997-01-01

Three types of turbulence models which account for rotational effects in noninertial frames of reference are evaluated for the case of incompressible, fully developed rotating turbulent channel flow. The different types of models are a Coriolis-modified eddy-viscosity model, a realizable algebraic stress model, and an algebraic stress model which accounts for dissipation rate anisotropies. A direct numerical simulation of a rotating channel flow is used for the turbulent model validation. This simulation differs from previous studies in that significantly higher rotation numbers are investigated. Flows at these higher rotation numbers are characterized by a relaminarization on the cyclonic or suction side of the channel, and a linear velocity profile on the anticyclonic or pressure side of the channel. The predictive performance of the three types of models are examined in detail, and formulation deficiencies are identified which cause poor predictive performance for some of the models. Criteria are identified which allow for accurate prediction of such flows by algebraic stress models and their corresponding Reynolds stress formulations.

Fabrication and characterization of semicircular detection electrodes for contactless conductivity detector - CE microchips.

PubMed

Lee, Chia-Yen; Chen, C M; Chang, Guan-Liang; Lin, Che-Hsin; Fu, Lung-Ming

2006-12-01

This study uses simple and reliable microfabrication techniques to fabricate CE biochips, integrating a novel contactless conductivity detector in a miniaturized detection system in a microfluidic biochip. The off-channel electrodes are deposited around side channels by Au sputtering and patterned using a standard "lift-off" process. A vacuum fusion bonding process is employed to seal the lower substrate containing the microchannels and the electrodes to an upper glass cover plate. The variations in the capacitance between the semicircular detection electrodes in the side channels are measured as different samples and ions pass through the detection region of the CE separation channel. Samples of Rhodamine B, commercial sports drinks, mineral waters, and a red wine, respectively, are mixed in different buffer solutions, separated, and successfully detected using the developed device. The semicircular detection electrodes for the contactless conductivity detector have microscale dimensions and provide a valuable contribution to the realization of the lab-on-a-chip concept.

Integration of both dense wavelength-division multiplexing and coarse wavelength-division multiplexing demultiplexer on one photonic crystal chip

NASA Astrophysics Data System (ADS)

Tian, Huiping; Shen, Guansheng; Liu, Weijia; Ji, Yuefeng

2013-07-01

An integrated model of photonic crystal (PC) demultiplexer that can be used to combine dense wavelength-division multiplexing (DWDM) and coarse wavelength-division multiplexing (CWDM) systems is first proposed. By applying the PC demultiplexer, dense channel spacing 0.8 nm and coarse channel spacing 20 nm are obtained at the same time. The transmission can be improved to nearly 90%, and the crosstalk can be decreased to less than -18 dB by enlarging the width of the bus waveguide. The total size of the device is 21×42 μm2. Four channels on one side of the demultiplexer can achieve DWDM in the wavelength range between 1575 and 1578 nm, and the other four channels on the other side can achieve CWDM in the wavelength range between 1490 and 1565 nm, respectively. The demonstrated demultiplexer can be applied in the future CWDM and DWDM system, and the architecture costs can be significantly reduced.

Experimental investigation of a scaled-up passive micromixer with uneven interdigital inlet and teardrop obstruction elements

NASA Astrophysics Data System (ADS)

Cook, Kristina J.; Fan, YanFeng; Hassan, Ibrahim

2012-05-01

Micromixers are vital components in micro total analysis systems. It is desirable to develop micromixers which are capable of rapidly mixing two or more fluids in a small footprint area, while minimizing mechanical losses. A novel planar scaled-up passive micromixer is experimentally investigated in this study. The design incorporates a 7-substream uneven interdigital inlet which supplies two liquid species in a parallel arrangement and promotes diffusion along the side walls. Forty-eight staggered teardrop-shaped obstruction elements located along the channel length combined with 32 side walls protrusions increase the two-fluid interfacial area while converging the flow due to periodic reductions in cross-sectional area. The scaled-up micromixer has a mixing channel length of 110 mm with a mixing channel height and width of 2 and 5 mm, respectively. Experimental investigations are carried out at four locations along the channel length and at Reynolds numbers equal to 1, 5, 10, 25, 50, and 100, where the Reynolds number is calculated based on total two-fluid flow and the mixing channel hydraulic diameter. Flow visualization is employed to study flow patterns, while induced fluorescence (IF), using de-ionized water and low concentration Rhodamine 6G solutions, provides mixing efficiency data. Results show a change in dominant mixing mechanism from mass diffusion to mass advection, with a critical Reynolds number of 25. At high Reynolds numbers, the formation of additional lamellae is observed, as is the formation of Dean vortices in the vicinity of the teardrop obstructions. Of the tested cases, the highest outlet mixing efficiency, 68.5%, is achieved at a Reynolds number of 1, where mass diffusion dominates. At low Reynolds numbers, superior mixing efficiency is due primarily to the implementation of the uneven interdigital inlet. A comparable mixing length is proposed to allow for reasonable comparison with published studies.

Three occurred debris flows in North-Eastern Italian Alps: documentation and modeling

NASA Astrophysics Data System (ADS)

Boreggio, Mauro; Gregoretti, Carlo; Degetto, Massimo; Bernard, Martino

2015-04-01

Three occurred events of debris flows are documented and modeled by back-analysis. The three debris flows events are those occurred at Rio Lazer on the 4th of November 1966, at Fiames on the 5th of July 2006 and at Rovina di Cancia on the 18th of July 2009. All the three sites are located in the North-Eastern Italian Alps. In all the events, runoff entrained sediments present on natural channels and formed a solid-liquid wave that routed downstream. The first event concerns the routing of debris flow on an inhabited fan. Map of deposition pattern of sediments are built by using post-events photos through stereoscopy techniques. The second event concerns the routing of debris flow along the main channel descending from Pomagagnon Fork. Due to the obstruction of the cross-section debris flow deviated from the original path on the left side and routed downstream by cutting a new channel on the fan. It dispersed in multiple paths when met the wooden area. Map of erosion and deposition depths are built after using a combination of Lidar and GPS data. The third event concerns the routing of debris flow in the Rovina di Cancia channel that filled the reservoir built at the end of the channel and locally overtopped the retaining wall on the left side. A wave of mud and debris inundated the area downstream the overtopping point. Map of erosion and deposition depths are obtained by subtracting two GPS surveys, pre and post event. All the three occurred debris flows are simulated by modeling runoff that entrained debris flow for determining the solid-liquid hydrograph downstream the triggering areas. The routing of the solid-liquid hydrograph was simulated by a bi-phase cell model based on the kinematic approach. The comparison between simulated and measured erosion and deposition depths is satisfactory. The same parameters for computing erosion and deposition were used for the three occurred events.

Quantifying the determinants of decremental response in critical ventricular tachycardia substrate.

PubMed

Beheshti, Mohammadali; Nayyar, Sachin; Magtibay, Karl; Massé, Stéphane; Porta-Sanchez, Andreu; Haldar, Shouvik; Bhaskaran, Abhishek; Vigmond, Edward; Nanthakumar, Kumaraswamy

2018-05-28

Decremental response evoked with extrastimulation (DEEP) is a useful tool for determining diastolic return path of ventricular tachycardia (VT). Though a targeted VT ablation is feasible with this approach, determinants of DEEP response have not been studied OBJECTIVES: To elucidate the effects of clinically relevant factors, specifically, the proximity of the stimulation site to the arrhythmogenic scar, stimulation wave direction, number of channels open in the scar, size of the scar and number of extra stimuli on decrement and entropy of DEEP potentials. In a 3-dimensional bi-domain simulation of human ventricular tissue (TNNP cell model), an irregular subendocardial myopathic region was generated. An irregular channel of healthy tissue with five potential entry branches was shaped into the myopathic region. A bipolar electrogram was derived from two electrodes positioned in the centre of the myopathic region. Evoked delays between far-field and local Electrogram (EGM) following an extrastimulus (S1-S2, 500-350 ms) were measured as the stimulation site, channel branches, and inexcitable tissue size were altered. Stimulation adjacent to the inexcitable tissue from the side opposite to the point-of-entry produces longest DEEP delay. The DEEP delay shortens when the stimulation point is farther away from the scar, and it decreases maximally when stimulation is done from a site beside a conduction barrier. Entropy increases with S2 when stimulation site is from farther away. An unprotected channel structure with multiple side-branch openings had shorter DEEP delay compared to a protected channel structure with a paucity of additional side-branch openings and a point-of-entry on the side opposite to the pacing source. Addition of a second shorter extrastimulus did not universally lead to higher DEEP delay CONCLUSIONS: Location and direction of the wavefront in relation to scar entry and size of scar determine the degree of evoked response while the number of extrastimuli has a small additional decremental effect. Copyright © 2018 Elsevier Ltd. All rights reserved.

On-chip gradient generation in 256 microfluidic cell cultures: simulation and experimental validation.

PubMed

Somaweera, Himali; Haputhanthri, Shehan O; Ibraguimov, Akif; Pappas, Dimitri

2015-08-07

A microfluidic diffusion diluter was used to create a stable concentration gradient for dose response studies. The microfluidic diffusion diluter used in this study consisted of 128 culture chambers on each side of the main fluidic channel. A calibration method was used to find unknown concentrations with 12% error. Flow rate dependent studies showed that changing the flow rates generated different gradient patterns. Mathematical simulations using COMSOL Multi-physics were performed to validate the experimental data. The experimental data obtained for the flow rate studies agreed with the simulation results. Cells could be loaded into culture chambers using vacuum actuation and cultured for long times under low shear stress. Decreasing the size of the culture chambers resulted in faster gradient formation (20 min). Mass transport into the side channels of the microfluidic diffusion diluter used in this study is an important factor in creating the gradient using diffusional mixing as a function of the distance. To demonstrate the device's utility, an H2O2 gradient was generated while culturing Ramos cells. Cell viability was assayed in the 256 culture chambers, each at a discrete H2O2 concentration. As expected, the cell viability for the high concentration side channels increased (by injecting H2O2) whereas the cell viability in the low concentration side channels decreased along the chip due to diffusional mixing as a function of distance. COMSOL simulations were used to identify the effective concentration of H2O2 for cell viability in each side chamber at 45 min. The gradient effects were confirmed using traditional H2O2 culture experiments. Viability of cells in the microfluidic device under gradient conditions showed a linear relationship with the viability of the traditional culture experiment. Development of the microfluidic device used in this study could be used to study hundreds of concentrations of a compound in a single experiment.

Probing pore constriction in a ligand-gated ion channel by trapping a metal ion in the pore upon agonist dissociation.

PubMed

Pittel, Ilya; Witt-Kehati, Dvora; Degani-Katzav, Nurit; Paas, Yoav

2010-08-20

Eukaryotic pentameric ligand-gated ion channels (pLGICs) are receptors activated by neurotransmitters to rapidly transport ions across cell membranes, down their electrochemical gradients. Recent crystal structures of two prokaryotic pLGICs were interpreted to imply that the extracellular side of the transmembrane pore constricts to close the channel (Hilf, R. J., and Dutzler, R. (2009) Nature 457, 115-118; Bocquet, N., Nury, H., Baaden, M., Le Poupon, C., Changeux, J. P., Delarue, M., and Corringer, P. J. (2009) Nature 457, 111-114). Here, we utilized a eukaryotic acetylcholine (ACh)-serotonin chimeric pLGIC that was engineered with histidines to coordinate a metal ion within the channel pore, at its cytoplasmic side. In a previous study, the access of Zn(2+) ions to the engineered histidines had been explored when the channel was either at rest (closed) or active (open) (Paas, Y., Gibor, G., Grailhe, R., Savatier-Duclert, N., Dufresne, V., Sunesen, M., de Carvalho, L. P., Changeux, J. P., and Attali, B. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 15877-15882). In this study, the interactions of Zn(2+) with the pore were probed upon agonist (ACh) dissociation that triggers the transition of the receptor from the active conformation to the resting conformation (i.e. during deactivation). Application of Zn(2+) onto ACh-bound open receptors obstructed their pore and prevented ionic flow. Removing ACh from its extracellular binding sites to trigger deactivation while Zn(2+) is still bound led to tight trapping of Zn(2+) within the pore. Together with single-channel recordings, made to explore single pore-blocking events, we show that dissociation of ACh causes the gate to shut on a Zn(2+) ion that effectively acts as a "foot in the door." We infer that, upon deactivation, the cytoplasmic side of the pore of the ACh-serotonin receptor chimera constricts to close the channel.

Status of the \\varvec{Λ (1405)}

NASA Astrophysics Data System (ADS)

Mai, Maxim

2018-07-01

I give an overview of the current status of the lowest s-wave baryon resonance in the strangeness (S=-1) channel, the Λ (1405). Recent results from Lattice QCD calculations and new high-precision data from photoproduction experiments are highlighted in this talk. On the theoretical side various directions have been explored over the last two decades on the basis of coupled-channel chiral unitary models. New photoproduction data can be used to reduce statistical uncertainty of the predictions of such models. As for the systematic uncertainties, a recent comparative analysis of modern approaches exhibits many similarities but also large ambiguities in some of the predicted properties of the antikaon-nucleon scattering amplitudes. Some possible ways to reduce such a model dependence are discussed at the end of this manuscript.

An Evaluation of Relative Damage to the Powertrain System in Tracked Vehicles

PubMed Central

Lee, Sang-Ho; Lee, Jeong-Hwan; Goo, Sang-Hwa; Cho, Yong-Cheol; Cho, Ho-Young

2009-01-01

The objective of this study was to improve the reliability of the endurance test for the powertrain system of military tracked vehicles. The measurement system that measures the driving duty applied to the powertrain system caused by mobility on roads consists of eight analog channels and two pulse channels, including the propeller shaft output torques for the left and right sides. The data obtained from this measurement system can be used to introduce a new technology that produces the output torque of a torque converter and that can be applied to analyze the revolution counting for the endurance and road mobility in the front unit and represent the relative fatigue damages analysis technique and its results according to the driven roads through a cumulative fatigue method. PMID:22573990

HYDROGEOMORPHIC INFLUENCES ON MACROPHYTES AS HABITAT IN GREAT LAKES WETLANDS

EPA Science Inventory

We used rapid survey techniques to map saubmergerd, floating and emergent vegetation in 10 coastal wetlands of Lake Superior. Density and structure of plant beds in "bay," "main channel," and "side channel" areas was evaluated from cover indices and presence/dominance by growth f...

Range resolution improvement in passive bistatic radars using nested FM channels and least squares approach

NASA Astrophysics Data System (ADS)

Arslan, Musa T.; Tofighi, Mohammad; Sevimli, Rasim A.; ćetin, Ahmet E.

2015-05-01

One of the main disadvantages of using commercial broadcasts in a Passive Bistatic Radar (PBR) system is the range resolution. Using multiple broadcast channels to improve the radar performance is offered as a solution to this problem. However, it suffers from detection performance due to the side-lobes that matched filter creates for using multiple channels. In this article, we introduce a deconvolution algorithm to suppress the side-lobes. The two-dimensional matched filter output of a PBR is further analyzed as a deconvolution problem. The deconvolution algorithm is based on making successive projections onto the hyperplanes representing the time delay of a target. Resulting iterative deconvolution algorithm is globally convergent because all constraint sets are closed and convex. Simulation results in an FM based PBR system are presented.

Stability of gas channels in a dense suspension in the presence of obstacles

NASA Astrophysics Data System (ADS)

Poryles, Raphaël; Varas, Germán; Vidal, Valérie

2017-06-01

We investigate experimentally the influence of a fixed obstacle on gas rising in a dense suspension. Air is injected at a constant flow rate by a single nozzle at the bottom center of a Hele-Shaw cell. Without obstacles, previous works have shown that a fluidized zone is formed with a parabolic shape, with a central air channel and two granular convection rolls on its sides. Here, we quantify the influence of the obstacle's shape, size, and height on the location and dynamics of the central air channel. Different regimes are reported: the air channel can simply deviate (stable), or it can switch sides over time (unstable), leading to two signatures not only above the obstacle, but sometimes also below it. This feedback also influences the channel deviation when bypassing the obstacle. A wake of less or no motion is reported above the largest obstacles as well as the maximum probability of gas location, which can be interesting for practical applications. The existence of a critical height hc≃7 cm is discussed and compared with the existence of an air finger that develops from the injection nozzle and is stable in time. A dimensionless number describing the transition between air fingering and fracturing makes it possible to predict the channel's stability.

A system shift in tidal choking due to the construction of Yangshan Harbour, Shanghai, China

NASA Astrophysics Data System (ADS)

Guo, Wenyun; Wang, Xiao Hua; Ding, Pingxing; Ge, Jianzhong; Song, Dehai

2018-06-01

Tidal choking is a geometric feature caused by a narrowed channel. Construction of the Yangshan Harbour, Shanghai, China obstructed three key channels and intensively changed the local geometry and topography. In this study nine numerical experiments based on the Finite-Volume Community Ocean Model are conducted to study the project's influence on tidal characteristics. Results show that stronger tidal choking happened at the East Entrance after project, mainly due to the jet induced water-level drop forced by Bernoulli law and the longer and narrower geometry. The stronger tidal choking forces a faster flow and larger tidal energy flux at the choked channel while reducing the tidal amplitude in the Inner Harbour Area (IHA). The scouring on this channel reduces the choking effect but further enlarges tidal energy flux. Moreover, damming the channels decrease the tidal amplitude at the lee side of tidal propagating direction while increasing the amplitude on the stoss side. The dams also decrease the tidal current on both sides, and meanwhile develop two patches with stronger current aside the dam. The project induced changes in tidal characteristics are complex in space, and perturbations in bathymetry increase this complexity. Yangshan Harbour's construction induces little changes in the total tidal energy density in the IHA, but induces obvious changes in the spatial distribution of tidal energy. Although this study is site-specific, the findings may be applicable to tidal dynamics in land reclamation close to open seas, such as the dramatic reclamation of islands in the South China Sea.

Effect of blue dye guided lymph channel ligation on the surgical morbidity of groin lymphadenectomy for vulval cancer: a feasibility study.

PubMed

Bharathan, Rasiah; Madhuri, Kavitha; Fish, Andrew; Larsen-Disney, Peter; Chatterjee, Jayanthan; Butler-Manuel, Simon; Tailor, Anil; Kehoe, Sean

2018-02-09

Inguinal lymphadenectomy has significant morbidity. Blue dye-guided lymph channel ligation is an effective technique for resolving lymphocele. This was a feasibility study in a preventative setting. Patients with vulval cancer requiring bilateral inguinal lymphadenectomy were recruited. After lymphadenectomy, patent blue V dye was injected and the severed lymph channels leaking blue dye, on the randomly-designated side were ligated. The median age was 72.5 years and the median body mass index was 25. The median lymph node harvest was 18.5. There were no significant surgical procedural differences between the right and the left sides. There was no significant difference between the two arms in terms of the duration or the volume of drainage and post-operative complications. All patients were alive at the follow-up period of 40.5 months. In this feasibility study, blue dye-guided lymph channel ligation did not significantly impact on post-operative outcomes. Impact statement What is already known on this subject? Lymph channel ligation with blue dye-guidance is an effective strategy for managing recalcitrant inguinal lymphocyst. This strategy was prospectively-studied in a small series of patients with non-gynaecological cancers. This particular study by Nakamura et al. ( 2011 ) revealed that such a strategy might be efficacious in reducing wound drain output. What do the results of this study add? Our study is the first study to assess this technique exclusively in vulval cancer. Blue dye-guided lymph channel ligation at the time of inguinal lymphadenectomy does not appear to reduce wound drainage. However, this study suggests that primary lymphocyst predominantly results from inflammatory exudates, whereas persistent secondary lymphocysts are likely to result from lymphorrhoea. What are the implications of these findings for clinical practice and/or further research? Future studies, which aim to reduce the morbidity of open inguinal lymphadenectomy, should employ a composite strategy to reduce inflammatory secretions. In addition, a biochemical and cytological analysis on lymphocysts at various time points should be performed to characterise the natural history of groin lymphocysts.

Quantum key distribution with prepare-and-measure Bell test

PubMed Central

Tan, Yong-gang

2016-01-01

The prepare-and-measure quantum key distribution (QKD) has the merits of fast speed, high key generation rate, and easy implementation. However, the detector side channel attacks greatly undermine the security of the key bits. The eavesdropper, Eve, exploits the flaws of the detectors to obtain illegal information without violating quantum principles. It means that she can intervene in the communication without being detected. A prepare-and-measure Bell test protocol will be proposed. By randomly carrying out Bell test at the side of the information receiver, Bob, Eve’s illegal information gain within the detector side channel attack can be well bounded. This protocol does not require any improvement on the detectors used in available prepare-and-measure QKD. Though we only illustrate its application in the BB84 protocol, it is applicable for any prepare-and-measure QKD. PMID:27733771

25. NORTH TRAINING WALL, EAST SECTION, SIDE WALL CONSTRUCTION, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

25. NORTH TRAINING WALL, EAST SECTION, SIDE WALL CONSTRUCTION, LOOKING WEST FROM A POINT ABOUT 500 FEET FROM THE MIDDLE HARBOR PARK FISHING PIER. (Panoramic view 1 of 2). - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

Observations of a tidal intrusion front in a tidal channel

NASA Astrophysics Data System (ADS)

Lu, Shasha; Xia, Xiaoming; Thompson, Charlie E. L.; Cao, Zhenyi; Liu, Yifei

2017-11-01

A visible front indicated by a surface colour change, and sometimes associated with foam or debris lines, was observed in a tidal channel during neap tide. This is an example of a tidal intrusion front occurring in the absence of sudden topographical changes or reversing flows, typically reported to be associated with such fronts. Detailed Acoustic Doppler Current Profiler and conductivity/temperature/depth measurements were taken on repeated transects both with fronts apparent and with fronts absent. The results indicated that the front occurred as a result of stratification, which was sustained by the buoyancy flux and the weak tide-induced mixing during neap ebb tide. The stronger tide-induced mixing during spring tide restrained stratification, leading to the absence of a front. The mechanism of the frontogenesis was the density gradient between the stratified water formed during neap ebb tide, and the more mixed seawater during neap flood tide; thus, the water on the landward (southwestern) side of the front was stratified, and that on the seaward side (northeastern) of the front was vertically well mixed. Gradient Richardson number estimates suggest that the flow between the stratified and mixed water was near the threshold 0.25 for shear instability. Meanwhile, the density gradient would provide an initial baroclinic contribution to velocity convergence, which is indicated by the accumulation of buoyant matter such as foam, grass, and debris into a sharply defined line along the surface. The front migrates with the flood current, with a local maximum towards the eastern side of the channel, leading to an asymmetrical shape with the eastern side of the front driven further into the Tiaozhoumen tidal channel.

Band-broadening suppressed effect in long turned geometry channel and high-sensitive analysis of DNA sample by using floating electrokinetic supercharging on a microchip.

PubMed

Xu, Zhongqi; Murata, Kenji; Arai, Akihiro; Hirokawa, Takeshi

2010-03-12

A featured microchip owning three big reservoirs and long turned geometry channel was designed to improve the detection limit of DNA fragments by using floating electrokinetic supercharging (FEKS) method. The novel design matches the FEKS preconcentration needs of a large sample volume introduction with electrokinetic injection (EKI), as well as long duration of isotachophoresis (ITP) process to enrich low concentration sample. In the curved channel [ approximately 45.6 mm long between port 1 (P1) and the intersection point of two channels], EKI and ITP were performed while the side port 3 (P3) was electrically floated. The turn-induced band broadening with or without ITP process was investigated by a computer simulation (using CFD-ACE+ software) when the analytes traveling through the U-shaped geometry. It was found that the channel curvature determined the extent of band broadening, however, which could be effectively eliminated by the way of ITP. After the ITP-stacked zones passed the intersection point from P1, they were rapidly destacked for separation and detection from ITP to zone electrophoresis by using leading ions from P3. The FEKS carried on the novel chip successfully contributed to higher sensitivities of DNA fragments in comparison with our previous results realized on either a single channel or a cross microchip. The analysis of low concentration 50 bp DNA step ladders (0.23 mugml after 1500-fold diluted) was achieved with normal UV detection at 260 nm. The obtained limit of detections (LODs) were on average 100 times better than using conventional pinched injection, down to several ngml for individual DNA fragment.

Evaluation of image quality of a 32-channel versus a 12-channel head coil at 1.5T for MR imaging of the brain.

PubMed

Parikh, P T; Sandhu, G S; Blackham, K A; Coffey, M D; Hsu, D; Liu, K; Jesberger, J; Griswold, M; Sunshine, J L

2011-02-01

Multichannel phased-array head coils are undergoing exponential escalation of coil element numbers. While previous technical studies have found gains in SNR and spatial resolution with the addition of element coils, it remains to be determined how these gains affect clinical reading. The purpose of this clinical study was to determine if the SNR and spatial resolution characteristics of a 32-channel head coil result in improvements in perceived image quality and lesion evaluation. Twenty-one patients underwent MR imaging of the brain at 1.5T sequentially with both a 12-channel and a 32-channel receive-only phased-array head coil. Axial T2WIs, T1WIs, FLAIR images, and DWIs were acquired. Anonymized images were compared side-by-side and by sequence for image quality, lesion evaluation, and artifacts by 3 neuroradiologists. Results of the comparison were analyzed for the preference for a specific head coil. FLAIR and DWI images acquired with the 32-channel coil showed significant improvement in image quality in several parameters. T2WIs also improved significantly with acquisition by the 32-channel coil, while T1WIs improved in a limited number of parameters. While lesion evaluation also improved with acquisition of images by the 32-channel coil, there was no apparent improvement in diagnostic quality. There was no difference in artifacts between the 2 coils. Improvements in SNR and spatial resolution attributed to image acquisition with a 32-channel head coil are paralleled by perceived improvements in image quality.

The alcohol supply: its importance to public health and safety, and essential research questions.

PubMed

Edwards, G; Holder, H D

2000-12-01

This paper seeks to identify the main questions which have emerged from the preceding papers concerning the supply side of alcohol. On any rational analysis these issues are of thoroughly legitimate concern to public health researchers. We list them under seven headings: (1) what drives the alcohol supply side? (2) What is the impact of alcohol supply on demand? (3) What are the benefits and risks to health and safety stemming from deregulation and what are the possibilities to avoid negative effects? (4) What is the size and significance of the alcohol supply which may derive from other than the officially approved channels? (5) Who are the important actors in the alcohol supply system? (6) What are local influences on alcohol supply and its distribution throughout communities? (7) What is to be learned from tobacco and other risky commodities about the importance of supply? Finally, we outline a series of possible next steps for a supply side initiative.

Impact of jammer side information on the performance of anti-jam systems

NASA Astrophysics Data System (ADS)

Lim, Samuel

1992-03-01

The Chernoff bound parameter, D, provides a performance measure for all coded communication systems. D can be used to determine upper-bounds on bit error probabilities (BEPs) of Viterbi decoded convolutional codes. The impact on BEP bounds of channel measurements that provide additional side information can also be evaluated with D. This memo documents the results of a Chernoff bound parameter evaluation in optimum partial-band noise jamming (OPBNJ) for both BPSK and DPSK modulation schemes. Hard and soft quantized receivers, with and without jammer side information (JSI), were examined. The results of this analysis indicate that JSI does improve decoding performance. However, a knowledge of jammer presence alone achieves a performance level comparable to soft decision decoding with perfect JSI. Furthermore, performance degradation due to the lack of JSI can be compensated for by increasing the number of levels of quantization. Therefore, an anti-jam system without JSI can be made to perform almost as well as a system with JSI.

Chlorophyll a and inorganic suspended solids in backwaters of the upper Mississippi River system: Backwater lake effects and their associations with selected environmental predictors

USGS Publications Warehouse

Rogala, James T.; Gray, Brian R.

2006-01-01

The Long Term Resource Monitoring Program (LTRMP) uses a stratified random sampling design to obtain water quality statistics within selected study reaches of the Upper Mississippi River System (UMRS). LTRMP sampling strata are based on aquatic area types generally found in large rivers (e.g., main channel, side channel, backwater, and impounded areas). For hydrologically well-mixed strata (i.e., main channel), variance associated with spatial scales smaller than the strata scale is a relatively minor issue for many water quality parameters. However, analysis of LTRMP water quality data has shown that within-strata variability at the strata scale is high in off-channel areas (i.e., backwaters). A portion of that variability may be associated with differences among individual backwater lakes (i.e., small and large backwater regions separated by channels) that cumulatively make up the backwater stratum. The objective of the statistical modeling presented here is to determine if differences among backwater lakes account for a large portion of the variance observed in the backwater stratum for selected parameters. If variance associated with backwater lakes is high, then inclusion of backwater lake effects within statistical models is warranted. Further, lakes themselves may represent natural experimental units where associations of interest to management may be estimated.

Laser-guided, intersecting discharge channels for the final beam transport in heavy-ion fusion

NASA Astrophysics Data System (ADS)

Niemann, C.; Neff, S.; Tauschwitz, A.; Penache, D.; Birkner, R.; Constantin, C.; Knobloch, R.; Presura, R.; Rosmej, F. B.; Hoffmann, D. H. H.; Yu, S. S.

2003-06-01

Ion-beam transport in space charge neutralizing discharge channels has been proposed for the final focus and chamber transport in a heavy-ion fusion reactor. A driver scenario with two-sided target illumination requires a system of two intersecting discharges to transport beams of the same charge from opposite sides towards the fusion target. In this article we report on experiments on the creation of free-standing, intersecting high-current discharge channels. The discharges are initiated in ammonia gas (NH3) in a metallic chamber by two perpendicular CO2-laser beams, which resonantly heat and subsequently rarefy the gas along the laser paths before the breakdown. These low density channels guide the discharges along the predefined paths and also around the 90° angles without any mechanical guiding structures. In this way stable X-, T-, and L-shaped discharges with currents in excess of 40 kA, at pressures of a few mbar were created with a total length of 110 cm. An 11.4 A MeV 58Ni+12 beam from the UNILAC (Universal Linear Accelerator) linear accelerator was used to probe the line-integrated ion-optical properties of the central channel in a T-shaped discharge.

Knudsen pump inspired by Crookes radiometer with a specular wall

NASA Astrophysics Data System (ADS)

Baier, Tobias; Hardt, Steffen; Shahabi, Vahid; Roohi, Ehsan

2017-03-01

A rarefied gas is considered in a channel consisting of two infinite parallel plates between which an evenly spaced array of smaller plates is arranged normal to the channel direction. Each of these smaller plates is assumed to possess one ideally specularly reflective and one ideally diffusively reflective side. When the temperature of the small plates differs from the temperature of the sidewalls of the channel, these boundary conditions result in a temperature profile around the edges of each small plate that breaks the reflection symmetry along the channel direction. This in turn results in a force on each plate and a net gas flow along the channel. The situation is analyzed numerically using the direct simulation Monte Carlo method and compared with analytical results where available. The influence of the ideally specularly reflective wall is assessed by comparing with simulations using a finite accommodation coefficient at the corresponding wall. The configuration bears some similarity to a Crookes radiometer, where a nonsymmetric temperature profile at the radiometer vanes is generated by different temperatures on each side of the vane, resulting in a motion of the rotor. The described principle may find applications in pumping gas on small scales driven by temperature gradients.

Fine-grained channel margin (FGCM) deposits conditioned by Large Woody Debris (LWD) in a gravel-bed river

NASA Astrophysics Data System (ADS)

Skalak, K. J.; Pizzuto, J. E.

2006-12-01

The purpose of this research is to examine the origin, occurrence, persistence, residence time and general significance of fine-grained channel margin storage in South River, a steep gravel-bedded stream in western Virginia. Fine-grained channel margin (FGCM) deposits in this study refers to specific in-channel deposits of mud and sand. These deposits occur primarily in the margins and near-banks regions of the channel. Fine- grained sediment storage in the near-bank regions is a result of reduced velocity caused by the bank obstructions. Nearly all of these obstructions consist of LWD accumulations in the channel. Storage occurs in four different geomorphic settings: 1) long pooled sections caused by bedrock or old mill dams, 2) the upstream ends of pools in channel margins with LWD accumulations, 3) bank obstructions usually caused by trees, 4) side channel backwaters where flow separates around islands. In approximately 38 km of river, there is 3000 m3 of fine-grained sediment stored in these features. The channel stores approximately 15 percent its total annual suspended load as fine-grained channel margin deposits. Consequently, these features represent a significant component of an annual sediment budget for this river. On average, the FGCM deposits are about 35 cm deep, 20 m long, and 4 m wide. They average 30 percent mud, 68 percent sand, and 2 percent gravel. These deposits have been cored and analyzed for Hg, grain size, loss-on-ignition, and bomb radiocarbon. Results from bomb radiocarbon analysis indicate that these features have an average age of 13 years. High Hg concentrations in fish tissue are an ongoing problem along South River, further motivating detailed study of these deposits.

Design of tubesheet for U-tube heat exchangers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paliwal, D.N.; Saxena, R.M.

1993-02-01

Thorough analysis of two-side integral tubesheet of U-tube heat exchanger is carried out, using Panc's component theory of plates. Effects of solid annular rim and interaction between tubesheet and shell/channel are considered. A design procedure based on foregoing analysis is proposed. Fictive elastic constants due to Osweiller, as well as effective elastic constants due to Slot and O'Donnell, are employed. Deformations, internal forces and primary stress intensities are evaluated in both pitch and diagonal directions. Stress category concept of ASME Sect. VIII Div. 2 is used. Design thickness obtained by this method is compared with the thicknesses calculated, using ASMEmore » Sect. VIII Div. 1, TEMA and BS-5500. This method enables us to calculate stresses in shell and channel in the junction region as well. Present analysis and design procedure thoroughly investigates the tubesheet behavior and leads to a thinner tubesheet. It is concluded that though all the codes based on Gardner's work provide safe and efficient design rules, and lie on firm footing, still there is further scope for reducing the design thickness of tubesheet by about ten percent.« less

Cross-talk between ATP-regulated K+ channels and Na+ transport via cellular metabolism in frog skin principal cells.

PubMed Central

Urbach, V; Van Kerkhove, E; Maguire, D; Harvey, B J

1996-01-01

Isolated frog skin epithelium, mounted in an Ussing chamber and bathed in standard NaCl Ringer solution, recycles K+ across the basolateral membrane of principal cells through an inward-rectifier K+ channel (Kir) operating in parallel with a Na+-K+-ATPase pump. Here we report on the metabolic control of the Kir channel using patch clamping, short-circuit current measurement and enzymatic determination of cellular (ATP (ATPi). 2. The constitutively active Kir channel in the basolateral membrane has the characteristics of an ATP-regulated K+ channel and is now classed as a KATP channel. In excised inside-out patches the open probability (Po) of KATP channels was reduced by ATPi with half-maximum inhibition at an ATPi concentration of 50 microM. 3. ATPi measured (under normal Na+ transport conditions) with luciferin-luciferase was 1.50 +/- 0.23 mM (mean +/- S.E.M.; range, 0.4-3.3 mM n = 11). Thus the KATP channel would be expected to be inactive in intact cells if ATPi was the sole regulator of channel activity. KATP channels which were inactivated by 1 mM ATPi in excised patches could be reactivated by addition of 100 microM ADP on the cytosolic side. When added alone, ADP blocks this channel with half-maximal inhibition at [ADPi] > 5 mM. 4. Sulphonylureas inhibit single KATP channels in cell-attached patches as well as the total basolateral K+ current measured in frog skin epithelia perforated with nystatin on the apical side. 5. Na+-K+-ATPase activity is a major determinant of cytosolic ATP. Blocking the pump activity with ouabain produced a time-dependent increase in ATPi and reduced the open probability of KATP channels in cell-attached membranes. 6. We conclude that the ratio of ATP/ADP is an important metabolic coupling factor between the rate of Na+-K+ pumping and K+ recycling. Images Figure 9 PMID:9011625

Experimental measurement-device-independent quantum digital signatures over a metropolitan network

NASA Astrophysics Data System (ADS)

Yin, Hua-Lei; Wang, Wei-Long; Tang, Yan-Lin; Zhao, Qi; Liu, Hui; Sun, Xiang-Xiang; Zhang, Wei-Jun; Li, Hao; Puthoor, Ittoop Vergheese; You, Li-Xing; Andersson, Erika; Wang, Zhen; Liu, Yang; Jiang, Xiao; Ma, Xiongfeng; Zhang, Qiang; Curty, Marcos; Chen, Teng-Yun; Pan, Jian-Wei

2017-04-01

Quantum digital signatures (QDSs) provide a means for signing electronic communications with information-theoretic security. However, all previous demonstrations of quantum digital signatures assume trusted measurement devices. This renders them vulnerable against detector side-channel attacks, just like quantum key distribution. Here we exploit a measurement-device-independent (MDI) quantum network, over a metropolitan area, to perform a field test of a three-party MDI QDS scheme that is secure against any detector side-channel attack. In so doing, we are able to successfully sign a binary message with a security level of about 10-7. Remarkably, our work demonstrates the feasibility of MDI QDSs for practical applications.

Geomorphic response to flow regulation and channel and floodplain alteration in the gravel-bedded Cedar River, Washington, USA

USGS Publications Warehouse

Gendaszek, Andrew S.; Magirl, Christopher S.; Czuba, Christiana R.

2012-01-01

Decadal- to annual-scale analyses of changes to the fluvial form and processes of the Cedar River in Washington State, USA, reveal the effects of flow regulation, bank stabilization, and log-jam removal on a gravel-bedded river in a temperate climate. During the twentieth century, revetments were built along ~ 60% of the lower Cedar River's length and the 2-year return period flow decreased by 47% following flow regulation beginning in 1914. The formerly wide, anastomosing channel narrowed by over 50% from an average of 47 m in 1936 to 23 m in 1989 and became progressively single threaded. Subsequent high flows and localized revetment removal contributed to an increase in mean channel width to about 34 m by 2011. Channel migration rates between 1936 and 2011 were up to 8 m/year in reaches not confined by revetments or valley walls and less than analysis uncertainty throughout most of the Cedar River's length where bank armoring restricted channel movement. In unconfined reaches where large wood and sediment can be recruited, contemporary high flows, though smaller in magnitude than preregulation high flows, form and maintain geomorphic features such as pools, gravel bars, and side channels. Reaches confined by revetments remain mostly unmodified in the regulated flow regime. While high flows are important for maintaining channel dynamics in the Cedar River, their effectiveness is currently reduced by revetments, limited sediment supply, the lack of large wood available for recruitment to the channel, and decreased magnitude since flow regulation.

The KATP channel in migraine pathophysiology: a novel therapeutic target for migraine.

PubMed

Al-Karagholi, Mohammad Al-Mahdi; Hansen, Jakob Møller; Severinsen, Johanne; Jansen-Olesen, Inger; Ashina, Messoud

2017-08-23

To review the distribution and function of K ATP channels, describe the use of K ATP channels openers in clinical trials and make the case that these channels may play a role in headache and migraine. K ATP channels are widely present in the trigeminovascular system and play an important role in the regulation of tone in cerebral and meningeal arteries. Clinical trials using synthetic K ATP channel openers report headache as a prevalent-side effect in non-migraine sufferers, indicating that K ATP channel opening may cause headache, possibly due to vascular mechanisms. Whether K ATP channel openers can provoke migraine in migraine sufferers is not known. We suggest that K ATP channels may play an important role in migraine pathogenesis and could be a potential novel therapeutic anti-migraine target.

Measurement-device-independent quantum key distribution.

PubMed

Lo, Hoi-Kwong; Curty, Marcos; Qi, Bing

2012-03-30

How to remove detector side channel attacks has been a notoriously hard problem in quantum cryptography. Here, we propose a simple solution to this problem--measurement-device-independent quantum key distribution (QKD). It not only removes all detector side channels, but also doubles the secure distance with conventional lasers. Our proposal can be implemented with standard optical components with low detection efficiency and highly lossy channels. In contrast to the previous solution of full device independent QKD, the realization of our idea does not require detectors of near unity detection efficiency in combination with a qubit amplifier (based on teleportation) or a quantum nondemolition measurement of the number of photons in a pulse. Furthermore, its key generation rate is many orders of magnitude higher than that based on full device independent QKD. The results show that long-distance quantum cryptography over say 200 km will remain secure even with seriously flawed detectors.

Time-domain digital pre-equalization for band-limited signals based on receiver-side adaptive equalizers.

PubMed

Zhang, Junwen; Yu, Jianjun; Chi, Nan; Chien, Hung-Chang

2014-08-25

We theoretically and experimentally investigate a time-domain digital pre-equalization (DPEQ) scheme for bandwidth-limited optical coherent communication systems, which is based on feedback of channel characteristics from the receiver-side blind and adaptive equalizers, such as least-mean-squares (LMS) algorithm and constant or multi- modulus algorithms (CMA, MMA). Based on the proposed DPEQ scheme, we theoretically and experimentally study its performance in terms of various channel conditions as well as resolutions for channel estimation, such as filtering bandwidth, taps length, and OSNR. Using a high speed 64-GSa/s DAC in cooperation with the proposed DPEQ technique, we successfully synthesized band-limited 40-Gbaud signals in modulation formats of polarization-diversion multiplexed (PDM) quadrature phase shift keying (QPSK), 8-quadrature amplitude modulation (QAM) and 16-QAM, and significant improvement in both back-to-back and transmission BER performances are also demonstrated.

Fabrication of Amorphous Indium Gallium Zinc Oxide Thin Film Transistor by using Focused Ion Beam

NASA Astrophysics Data System (ADS)

Zhu, Wencong

Compared with other transparent semiconductors, amorphous indium gallium zinc oxide (a-IGZO) has both good uniformity and high electron mobility, which make it as a good candidate for displays or large-scale transparent circuit. The goal of this research is to fabricate alpha-IGZO thin film transistor (TFT) with channel milled by focused ion beam (FIB). TFTs with different channel geometries can be achieved by applying different milling strategies, which facilitate modifying complex circuit. Technology Computer-Aided Design (TCAD) was also introduced to understand the effect of trapped charges on the device performance. The investigation of the trapped charge at IGZO/SiO2 interface was performed on the IGZO TFT on p-Silicon substrate with thermally grown SiO2 as dielectric. The subgap density-of-state model was used for the simulation, which includes conduction band-tail trap states and donor-like state in the subgap. The result shows that the de-trapping and donor-state ionization determine the interface trapped charge density at various gate biases. Simulation of IGZO TFT with FIB defined channel on the same substrate was also applied. The drain and source were connected intentionally during metal deposition and separated by FIB milling. Based on the simulation, the Ga ions in SiO2 introduced by the ion beam was drifted by gate bias and affects the saturation drain current. Both side channel and direct channel transparent IGZO TFTs were fabricated on the glass substrate with coated ITO. Higher ion energy (30 keV) was used to etch through the substrate between drain and source and form side channels at the corner of milled trench. Lower ion energy (16 keV) was applied to stop the milling inside IGZO thin film and direct channel between drain and source was created. Annealing after FIB milling removed the residual Ga ions and the devices show switch feature. Direct channel shows higher saturation drain current (~10-6 A) compared with side channel (~10-7 A) because of its shorter channel length and wider width, however, it also exhibit higher gate leakage current (>10-7 A) than side channel (<10-7 A) due to larger Ga ion implantation and diffusion region in SiO2 after annealing. Hysteresis window increase and positive VON shift were also observed due to the interface trap density increase and carrier density suppression both by Ga ions. Laser interference lithography was applied to define the IGZO active region, which gives more flexibility on TFT channel dimension and circuit modification. He-Cd laser with 325 nm wavelength was used to define 2D array of IGZO islands with period of 2.5 im. Logic gate array was designed and fabricated by combining this 2D array of IGZO islands and FIB direct channel milling. After annealing, device shows on-off feature, but high temperature (400 °C) release more free carrier and results in negative shift of VON. The row selection voltage was also introduced in the design of logic gate array to act as switch of input signals to each row separately. However, due to the long input signal sweeping time, the leakage current cannot be overlooked. The idea can be verified by AC or short pulse input signal.

Applied light-side coupling with optimized spiral-patterned zinc oxide nanorod coatings for multiple optical channel alcohol vapor sensing

NASA Astrophysics Data System (ADS)

Bin Abdul Rahim, Hazli Rafis; Bin Lokman, Muhammad Quisar; Harun, Sulaiman Wadi; Hornyak, Gabor Louis; Sterckx, Karel; Mohammed, Waleed Soliman; Dutta, Joydeep

2016-07-01

The width of spiral-patterned zinc oxide (ZnO) nanorod coatings on plastic optical fiber (POF) was optimized theoretically for light-side coupling and found to be 5 mm. Structured ZnO nanorods were grown on large core POFs for the purpose of alcohol vapor sensing. The aim of the spiral patterns was to enhance signal transmission by reduction of the effective ZnO growth area, thereby minimizing light leakage due to backscattering. The sensing mechanism utilized changes in the output signal due to adsorption of methanol, ethanol, and isopropanol vapors. Three spectral bands consisting of red (620 to 750 nm), green (495 to 570 nm), and blue (450 to 495 nm) were applied in measurements. The range of relative intensity modulation (RIM) was determined to be for concentrations between 25 to 300 ppm. Methanol presented the strongest response compared to ethanol and isopropanol in all three spectral channels. With regard to alcohol detection RIM by spectral band, the green channel demonstrated the highest RIM values followed by the blue and red channels, respectively.

Four-channel temperature and humidity microwave scanning radiometer

NASA Astrophysics Data System (ADS)

Xu, Pei-Yuan

1994-06-01

A compact four-channel microwave scanning radiometer for tropospheric remote sensing is being developed. A pair of 53.85 and 56.02 GHz and a pair of 23.87 and 31.65 GHz are adopted as temperature and humidity channels' frequencies respectively. For each pair of frequencies it has an offset reflector antenna and a Dicke-switching receiver. The pair of receivers is assembled in an enclosure, which is mounted on the rotating table of an azimuth mounting and the pair of antennas is connected with the rotating table of an azimuth mounting in the opposite side by a pair of elevation arms. Each antenna is composed of a 90 degree off-set paraboloid and a conical corrugated horn. Each antenna patterrn of four channels has nearly same HPBW, low side lobes, and low VSWR. The dual band humidity receiver is a time sharing type with 0.2K sensitivity at 1-sec integration time. The dual band temperature receiver is a band sharing type with 0.2K sensitivity at 10-sec integration time. The radiometer and observation are controlled by a single chip microcomputer to realize the unattended operation.

Cloud Top Scanning radiometer (CTS): User's guide

NASA Technical Reports Server (NTRS)

Brown, K. S.

1981-01-01

The CTS maps the Earth's surface with a resolution of 0.1 km from an altitude of 18km with 60km side-to-side coverage of the field. It has three spectral channels. The 0.625 micrometer centered visual channel detects reflectance to within 1 percent. The 6.75 micrometer centered water vapor channel detects changes in temperature of less than one degree Kelvin at 175 K. The 11.5 micrometer centered infrared window channel detects changes of less one half degree Kelvin at 175 K. The data can be converted graphically into three display images of the scene. Values for scene temperature and albedo are calculated from calibration equations. The equations were derived from in-situ and laboratory measurements. Intercomparisons of the flight data temperatures with ground based and other remote sensor results established the certainty of the derived temperature values to within 3 K over a wide temperature range (180 to 320 K). The system performance, calibration, and operation is successful and the engineering information describing this system should prove useful to scientists and potential users of the data.

Inhibition of human Na(v)1.5 sodium channels by strychnine and its analogs.

PubMed

Yuan, Chunhua; Sun, Lirong; Zhang, Meng; Li, Shuji; Wang, Xuemin; Gao, Tianming; Zhu, Xinhong

2011-08-15

Strychnine and brucine from the seeds of the plant Strychnos nux vomica have been shown to have interesting pharmacological effects on several neurotransmitter receptors. In this study, we have characterized the pharmacological properties of strychnine and its analogs on human Na(v)1.5 channels to assess their potential therapeutic advantage in certain arrhythmias. Among the eight alkaloids, only strychnine and icajine exhibited inhibition potency on the Na(v)1.5 channel with the half-maximum inhibition (IC(50)) values of 83.1μM and 104.6μM, respectively. Structure-function analysis indicated that the increased bulky methoxy groups on the phenyl ring or the negatively charged oxygen atom may account for this lack of inhibition on the Na(v)1.5 channel. Strychnine and icajine may bind to the channel by cation-π interactions. The substitution with a large side chain on the phenyl ring or the increased molecular volume may alter the optimized position for the compound close to the binding sites of the channel. Strychnine and icajine bind to the Na(v)1.5 channel with a new mechanism that is different from TTX and local anesthetics. They bind to the outer vestibule of the channel pore with fast association and dissociation rates at resting state. Strychnine and icajine had little effect on steady-state fast inactivation but markedly shifted the slow inactivation of Na(v)1.5 currents toward more hyperpolarized potentials. The property of icajine influencing slow-inactivated state of Na(v)1.5 channel would be potential therapeutic advantages in certain arrhythmias. Copyright © 2011 Elsevier Inc. All rights reserved.

Computer simulation of ion channel gating: the M(2) channel of influenza A virus in a lipid bilayer

NASA Technical Reports Server (NTRS)

Schweighofer, K. J.; Pohorille, A.

2000-01-01

The transmembrane fragment of the influenza virus M(2) protein forms a homotetrameric channel that transports protons. In this paper, we use molecular dynamics simulations to help elucidate the mechanism of channel gating by four histidines that occlude the channel lumen in the closed state. We test two competing hypotheses. In the "shuttle" mechanism, the delta nitrogen atom on the extracellular side of one histidine is protonated by the incoming proton, and, subsequently, the proton on the epsilon nitrogen atom is released on the opposite side. In the "water-wire" mechanism, the gate opens because of electrostatic repulsion between four simultaneously biprotonated histidines. This allows for proton transport along the water wire that penetrates the gate. For each system, composed of the channel embedded in a hydrated phospholipid bilayer, a 1.3-ns trajectory was obtained. It is found that the states involved in the shuttle mechanism, which contain either single-protonated histidines or a mixture of single-protonated histidines plus one biprotonated residue, are stable during the simulations. Furthermore, the orientations and dynamics of water molecules near the gate are conducive to proton transfer. In contrast, the fully biprotonated state is not stable. Additional simulations show that if only two histidines are biprotonated, the channel deforms but the gate remains closed. These results support the shuttle mechanism but not the gate-opening mechanism of proton gating in M(2).

Sodium channel blockers as therapeutic target for treating epilepsy: recent updates.

PubMed

Zuliani, Valentina; Fantini, Marco; Rivara, Mirko

2012-01-01

The voltage-gated sodium channels (VGSCs) are a family of membrane proteins forming a pore, through which they selectively conduct sodium ions inward and outward cell's plasma membranes in response to variations of membrane potentials, playing a fundamental role in controlling cellular excitability. Growing evidences suggest that abnormal VGSCs are involved in the pathophysiology of both acquired and inherited epilepsy. Approximately two dozen drugs are currently marketed for the treatment of epilepsy and most of them act as sodium channel blockers, preventing the return of the channels to the active state by stabilizing the inactive form. Despite the many drugs on the market, 30% of patients continue to experience seizures even in the presence of optimal doses of AEDs, while others continue to suffer from medication induced side effects. Thus, there is a great need to continue the search for new AEDs that are not only more effective, but also have a better side effects profile. For this reason, many efforts have been made in the recent years to identify new sodium channel blockers for the treatment of epilepsy. These studies have led to different classes of compounds, characterized by a great structural diversity. The aim of this review is to provide an introduction on the structure and function of the sodium channels, followed by a brief historical perspective on the sodium channel blockers in use as anticonvulsant drugs. Moreover, it will focus on the medicinal chemistry of the sodium channel blockers recently published (2008-2011) and the drug design/molecular modeling studies related to the receptor.

Use of laboratory studies to develop a dispersal model for Missouri River pallid sturgeon early life intervals

USGS Publications Warehouse

Kynard, B.; Parker, E.; Pugh, D.; Parker, T.

2007-01-01

Understanding the drift dynamics of pallid sturgeon (Scaphirhynchus albus) early life intervals is critical to evaluating damming effects on sturgeons. However, studying dispersal behavior is difficult in rivers. In stream tanks, we studied the effect of velocity on dispersal and holding ability, estimated swimming height, and used the data to estimate drift distance of pallid sturgeon. Dispersal was by days 0-10 embryos until fish developed into larvae on day 11 after 200 CTU (daily cumulative temperature units). Embryos in tanks with a mean channel velocity of 30.1 cm s-1 and a side eddy could not hold position in the eddy, so current controlled dispersal. Late embryos (days 6-10 fish) dispersed more passes per hour than early embryos (days 0-5 fish) and held position in side eddies when channel velocities were 17.3 cm s-1 or 21.1 cm s-1. Day and night swim-up and drift by embryos is an effective adaptation to disperse fish in channel flow and return fish from side eddies to the channel. Early embryos swam <0.50 cm above the bottom and late embryos swam higher (mean, 90 cm). A passive drift model using a near bottom velocity of 32 cm s-1 predicted that embryos dispersing for 11 days in channel flow would travel 304 km. Embryos spawned at Fort Peck Dam, Missouri River, must stop dispersal in <330 km or enter Lake Sakakawea, where survival is likely poor. The model suggests there may be a mismatch between embryo dispersal distance and location of suitable rearing habitat. This situation may be common for pallid sturgeon in dammed rivers. ?? 2007 Blackwell Verlag.

Voltage-dependent and -independent titration of specific residues accounts for complex gating of a ClC chloride channel by extracellular protons

PubMed Central

Niemeyer, María Isabel; Cid, L Pablo; Yusef, Yamil R; Briones, Rodolfo; Sepúlveda, Francisco V

2009-01-01

The ClC transport protein family comprises both Cl− ion channel and H+/Cl− and H+/NO3− exchanger members. Structural studies on a bacterial ClC transporter reveal a pore obstructed at its external opening by a glutamate side-chain which acts as a gate for Cl− passage and in addition serves as a staging post for H+ exchange. This same conserved glutamate acts as a gate to regulate Cl− flow in ClC channels. The activity of ClC-2, a genuine Cl− channel, has a biphasic response to extracellular pH with activation by moderate acidification followed by abrupt channel closure at pH values lower than ∼7. We have now investigated the molecular basis of this complex gating behaviour. First, we identify a sensor that couples extracellular acidification to complete closure of the channel. This is extracellularly-facing histidine 532 at the N-terminus of transmembrane helix Q whose neutralisation leads to channel closure in a cooperative manner. We go on to show that acidification-dependent activation of ClC-2 is voltage dependent and probably mediated by protonation of pore gate glutamate 207. Intracellular Cl− acts as a voltage-independent modulator, as though regulating the pKa of the protonatable residue. Our results suggest that voltage dependence of ClC-2 is given by hyperpolarisation-dependent penetration of protons from the extracellular side to neutralise the glutamate gate deep within the channel, which allows Cl− efflux. This is reminiscent of a partial exchanger cycle, suggesting that the ClC-2 channel evolved from its transporter counterparts. PMID:19153159

Fusion of Night Vision and Thermal Images

DTIC Science & Technology

2006-12-01

with the walls of the MCP channels. Thus, a thin metal oxide coating commonly known as an ion barrier film is added to the input side of the MCP to...with film ion barrier to filmless gated tubes. An important improvement for Gen 4 products is a greater target identification range and higher target...Metal Seals with S-25 Cathode Mircro-channel plate Ceramic/Metal Seals with GaAS Cathode Mircro-channel plate with ion barrier film Ceramic

Selective potentiation of 2-APB-induced activation of TRPV1–3 channels by acid

PubMed Central

Gao, Luna; Yang, Pu; Qin, Peizhong; Lu, Yungang; Li, Xinxin; Tian, Quan; Li, Yang; Xie, Chang; Tian, Jin-bin; Zhang, Chengwei; Tian, Changlin; Zhu, Michael X.; Yao, Jing

2016-01-01

Temperature-sensitive TRP channels are important for responses to pain and inflammation, to both of which tissue acidosis is a major contributing factor. However, except for TRPV1, acid-sensing by other ThermoTRP channels remains mysterious. We show here that unique among TRPV1–3 channels, TRPV3 is directly activated by protons from cytoplasmic side. This effect is very weak and involves key cytoplasmic residues L508, D512, S518, or A520. However, mutations of these residues did not affect a strong proton induced potentiation of TRPV3 currents elicited by the TRPV1–3 common agonist, 2-aminoethoxydiphenyl borate (2-APB), no matter if the ligand was applied from extracellular or cytoplasmic side. The acid potentiation was common among TRPV1–3 and only seen with 2-APB-related ligands. Using 1H-nuclear magnetic resonance to examine the solution structures of 2-APB and its analogs, we observed striking structural differences of the boron-containing compounds at neutral/basic as compared to acidic pH, suggesting that a pH-dependent configuration switch of 2-APB-based drugs may underlie their functionality. Supporting this notion, protons also enhanced the inhibitory action of 2-APB on TRPM8. Collectively, our findings reveal novel insights into 2-APB action on TRP channels, which should facilitate the design of new drugs for these channels. PMID:26876731

Probing the effects of the ester functional group, alkyl side chain length and anions on the bulk nanostructure of ionic liquids: a computational study.

PubMed

Fakhraee, Mostafa; Gholami, Mohammad Reza

2016-04-14

The effects of ester addition on nanostructural properties of biodegradable ILs composed of 1-alkoxycarbonyl-3-alkyl-imidazolium cations ([C1COOCnC1im](+), n = 1, 2, 4) combined with [Br](-), [NO3](-), [BF4](-), [PF6](-), [TfO](-), and [Tf2N](-) were explored by using the molecular dynamics (MD) simulations and quantum theory of atoms in molecules (QTAIM) analysis at 400 K. Various thermodynamic properties of these ILs were extensively computed in our earlier work (Ind. Eng. Chem. Res., 2015, 54, 11678-11700). Nano-scale segregation analysis demonstrates the formation of a small spherical island-like hydrocarbon within the continuous ionic domain for ILs with short alkyl side chain ([C1COOC1C1im]), and a sponge-like nanostructure for the compound with long alkyl side chain ([C1COOC4C1im]). Ester-functionalized ILs with ethyl side chain ([C1COOC2C1im]) are the turning point between two different morphologies. Non-polar channels were observed for [C1COOC4C1im] ILs composed of smaller anions such as [Br] and [NO3], whereas clustering organization was found for the other anions. Formation of the spherical micelle-like nanostructure was seen for lengthened cations. Finally, the incorporation of an ester group into the alkyl side chain of the cation leads to stronger segregation between charged and uncharged networks, which consequently increased the possibility of self-assembly and micelle formation.

Effects of channel constriction on upstream steering of flow around Locke Island, Columbia River, Washington

NASA Astrophysics Data System (ADS)

Loy, G. E.; Furbish, D. J.; Covey, A.

2010-12-01

Landsliding of the White Bluffs along the Columbia River in Washington State has constricted the width of the river on one side of Locke Island, a two-kilometer long island positioned in the middle of the channel. Associated changes in flow are thought to be causing relatively rapid erosion of Locke Island on the constricted side. This island is of cultural significance to Native American tribes of south-central Washington, so there are social as well as scientific reasons to understand how the alteration of stream channel processes resulting from the landsliding might be influencing observed erosion rates. Simple hydrodynamic calculations suggest that the constriction on one side of the island creates an upstream backwater effect. As a consequence a cross-stream pressure gradient upstream of the island results in steering of flow around the island into the unobstructed thread. This diversion of water decreases the discharge through the constriction. Therefore, flow velocities within the constriction are not necessarily expected to be higher than those in the unobstructed thread, contrary to initial reports suggesting that higher velocities within the constriction are the main cause of erosion. We set up streamtable experiments with lapse rate imaging to illustrate the backwater effects of the channel constriction and the associated cross-stream steering of flow around a model island. Our experiments are scaled by channel roughness and slope rather than geometrically, as the main focus is to understand the mechanical behavior of flow in this type of island-landslide system. In addition, we studied the stream velocities and flow steering as well as the magnitude of the backwater effect in both the constricted and unobstructed channels using tracer particles in the time-lapse images. These experimental data are compared with calculated upstream backwater distances determined from the known water-surface slope, flow depth, total discharge, and bed roughness. Furthermore, this experimental work will inform subsequent numerical modeling of flow and field-based measurements at Locke Island.

Apparatus for transporting hazardous materials

DOEpatents

Osterman, Robert A.; Cox, Robert

1992-01-01

An apparatus and method are provided for selectively receiving, transporting, and releasing one or more radioactive or other hazardous samples for analysis on a differential thermal analysis (DTA) apparatus. The apparatus includes a portable sample transporting apparatus for storing and transporting the samples and includes a support assembly for supporting the transporting apparatus when a sample is transferred to the DTA apparatus. The transporting apparatus includes a storage member which includes a plurality of storage chambers arrayed circumferentially with respect to a central axis. An adjustable top door is located on the top side of the storage member, and the top door includes a channel capable of being selectively placed in registration with the respective storage chambers thereby permitting the samples to selectively enter the respective storage chambers. The top door, when closed, isolates the respective samples within the storage chambers. A plurality of spring-biased bottom doors are located on the bottom sides of the respective storage chambers. The bottom doors isolate the samples in the respective storage chambers when the bottom doors are in the closed position. The bottom doors permit the samples to leave the respective storage chambers from the bottom side when the respective bottom doors are in respective open positions. The bottom doors permit the samples to be loaded into the respective storage chambers after the analysis for storage and transport to a permanent storage location.

Phenomenological and statistical analyses of turbulence in forced convection with temperature-dependent viscosity under non-Boussinesq condition.

PubMed

Yahya, S M; Anwer, S F; Sanghi, S

2013-10-01

In this work, Thermal Large Eddy Simulation (TLES) is performed to study the behavior of weakly compressible Newtonian fluids with anisotropic temperature-dependent viscosity in forced convection turbulent flow. A systematic analysis of variable-viscosity effects, isolated from gravity, with relevance to industrial cooling/heating applications is being carried out. A LES of a planar channel flow with significant heat transfer at a low Mach number was performed to study effects of fluid property variation on the near-wall turbulence structure. In this flow configuration the top wall is maintained at a higher temperature (T hot ) than the bottom wall (T cold ). The temperature ratio (R θ = T hot /T cold ) is fixed at 1.01, 2 and 3 to study the effects of property variations at low Mach number. Results indicate that average and turbulent fields undergo significant changes. Compared with isothermal flow with constant viscosity, we observe that turbulence is enhanced in the cold side of the channel, characterized by locally lower viscosity whereas a decrease of turbulent kinetic energy is found at the hot wall. The turbulent structures near the cold wall are very short and densely populated vortices but near the hot wall there seems to be a long streaky structure or large elongated vortices. Spectral study reveals that turbulence is completely suppressed at the hot side of the channel at a large temperature ratio because no inertial zone is obtained (i.e. index of Kolmogorov scaling law is zero) from the spectra in these region.

Diadenosine tetraphosphate-induced inhibition of ATP-sensitive K+ channels in patches excised from ventricular myocytes.

PubMed Central

Jovanovic, A.; Terzic, A.

1996-01-01

Diadenosine 5',5''-P1,P4-tetraphosphate (Ap4A) has been termed 'alarmone' due to its role in intracellular signaling during metabolic stress. It is not known whether Ap4A could modulate ATP-sensitive K+ (KATP) channels, a family of channels regulated by the metabolic status of a cell. We applied the single-channel patch-clamp technique to measure the effect of Ap4A on KATP channels. When applied to the intracellular side of patches, excised from guinea-pig ventricular myocytes, Ap4A inhibited KATP channel activity, in a reversible and concentration-dependent (half-maximal concentration approximately 17 microM) manner. We conclude that Ap4A, a naturally occurring diadenosine polyphosphate, is actually an inhibitor of the myocardial KATP channel. PMID:8789372

Integrated fountain effect pump device for fluid management at low gravity

NASA Technical Reports Server (NTRS)

Yuan, S. W. K.; Frank, D. J.

1988-01-01

A new device for fluid management at low gravity is described. The system is basically the same as the enclosed capillary device using screens, in which the screens along the gallery channels are replaced by porous plugs which are responsible for both the fluid retention and pumping of He II; in this device, no downstream pump is needed. The plugs in contact with liquid He on both sides act as a fountain-effect pumps (FEPs), while plugs exposed to vapor on one side behave as vapor-liquid phase separators (VLPSs). The total net rate of He II transfer into the receiving tank equals the mass flow rate through the FEP plugs minus the liquid loss from the VLPS plugs. The results of the performance analysis of this integrated FEP device are presented together with its schematic diagram.

Separation of platelets from whole blood using standing surface acoustic waves in a microchannel.

PubMed

Nam, Jeonghun; Lim, Hyunjung; Kim, Dookon; Shin, Sehyun

2011-10-07

Platelet separation from blood is essential for biochemical analyses and clinical diagnosis. In this article, we propose a method to separate platelets from undiluted whole blood using standing surface acoustic waves (SSAWs) in a microfluidic device. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with interdigitated transducer (IDT) electrodes patterned on a piezoelectric substrate. To avoid shear-induced activation of platelets, the blood sample flow was hydrodynamically focused by introducing sheath flow from two side-inlets and pressure nodes were designed to locate at side walls. By means of flow cytometric analysis, the RBC clearance ratio from whole blood was found to be over 99% and the purity of platelets was close to 98%. Conclusively, the present technique using SSAWs can directly separate platelets from undiluted whole blood with higher purity than other methods.

A numerical study of the supercritical CO2 plate heat exchanger subject to U-type, Z-type, and multi-pass arrangements

NASA Astrophysics Data System (ADS)

Zhu, Chen-Xi; Wang, Chi-Chuan

2018-01-01

This study proposes a numerical model for plate heat exchanger that is capable of handling supercritical CO2 fluid. The plate heat exchangers under investigation include Z-type (1-pass), U-type (1-pass), and 1-2 pass configurations. The plate spacing is 2.9 mm with a plate thickness of 0.8 mm, and the size of the plate is 600 mm wide and 218 mm in height with 60 degrees chevron angle. The proposed model takes into account the influence of gigantic change of CO2 properties. The simulation is first compared with some existing data for water-to-water plate heat exchangers with good agreements. The flow distribution, pressure drop, and heat transfer performance subject to the supercritical CO2 in plate heat exchangers are then investigated. It is found that the flow velocity increases consecutively from the entrance plate toward the last plate for the Z-type arrangement, and this is applicable for either water side or CO2 side. However, the flow distribution of the U-type arrangement in the water side shows opposite trend. Conversely, the flow distribution for U-type arrangement of CO2 depends on the specific flow ratio (C*). A lower C* like 0.1 may reverse the distribution, i.e. the flow velocity increases moderately alongside the plate channel like Z-type while a large C* of 1 would resemble the typical distribution in water channel. The flow distribution of CO2 side at the first and last plate shows a pronounced drop/surge phenomenon while the channels in water side does not reveal this kind of behavior. The performance of 2-pass plate heat exchanger, in terms of heat transfer rate, is better than that of 1-pass design only when C* is comparatively small (C* < 0.5). Multi-pass design is more effective when the dominant thermal resistance falls in the CO2 side.

Shall we trust WDDL?

NASA Astrophysics Data System (ADS)

Guilley, Sylvain; Chaudhuri, Sumanta; Sauvage, Laurent; Graba, Tarik; Danger, Jean-Luc; Hoogvorst, Philippe; Vong, Vinh-Nga; Nassar, Maxime; Flament, Florent

Security is not only a matter of cryptographic algorithms robustness but becomes also a question of securing their implementation. P. Kocher’s differential power analysis (DPA) is one of the many side-channel attacks that are more and more studied by the security community. Indeed, side-channel attacks (SCA) have proved to be very powerful on cryptographic algorithms such as DES and AES, customarily implemented in a wide variety of devices, ranging from smart-cards or ASICs to FPGAs. Among the proposed countermeasures, the “dual-rail with precharge logic” (DPL) aims at hiding information leaked by the circuit by making the power consumption independent of the calculation. However DPL logic could be subject to second order attacks exploiting timing difference between dual nets. In this article, we characterize by simulation, the vulnerability due to timing unbalance in the eight DES substitution boxes implemented in DPL WDDL style. The characterization results in a classification of the nodes according to their timing unbalance. Our results show that the timing unbalance is a major weakness of the WDDL logic, and that it could be used to retrieve the key using a DPA attack. This vulnerability has been experimentally observed on a full DES implementation using WDDL style for Altera Stratix EP1S25 FPGA.

Practical passive decoy state measurement-device-independent quantum key distribution with unstable sources.

PubMed

Liu, Li; Guo, Fen-Zhuo; Wen, Qiao-Yan

2017-09-12

Measurement-device-independent quantum key distribution (MDI-QKD) with the active decoy state method can remove all detector loopholes, and resist the imperfections of sources. But it may lead to side channel attacks and break the security of QKD system. In this paper, we apply the passive decoy state method to the MDI-QKD based on polarization encoding mode. Not only all attacks on detectors can be removed, but also the side channel attacks on sources can be overcome. We get that the MDI-QKD with our passive decoy state method can have a performance comparable to the protocol with the active decoy state method. To fit for the demand of practical application, we discuss intensity fluctuation in the security analysis of MDI-QKD protocol using passive decoy state method, and derive the key generation rate for our protocol with intensity fluctuation. It shows that intensity fluctuation has an adverse effect on the key generation rate which is non-negligible, especially in the case of small data size of total transmitting signals and long distance transmission. We give specific simulations on the relationship between intensity fluctuation and the key generation rate. Furthermore, the statistical fluctuation due to the finite length of data is also taken into account.

Modeling of flood-deposited sand distributions in a reach of the Colorado River below the Little Colorado River, Grand Canyon, Arizona

USGS Publications Warehouse

Wiele, S.M.

1998-01-01

A release from Glen Canyon Dam during March-April 1996 was designed to test the effectiveness with which the riparian environment could be renewed with discharges greatly in excess of the normal powerplant-restricted maximum. Of primary concern was the rebuilding of sand deposits along the channel sides that are important to the flora and fauna along the river corridor and that provide the only camp sites for riverside visitors to the Grand Canyon National Park. Analysis of the depositional processes with a model of flow, sand transport, and bed evolution shows that the sand deposits formed along the channel sides early during the high flow were affected only slightly by the decline in suspended-sand concentrations over the course of the controlled flood. Modeling results suggest that the removal of a large sand deposit over several hours was not a response to declining suspended-sand concentrations. Comparisons of the controlled-flood deposits with deposits formed during a flood in January 1993 on the Little Colorado River that contributed sufficient sand to raise the suspended-sand concentrations to predam levels in the main stem show that the depositional pattern as well as the magnitude is strongly influenced by the suspended-sand concentrations.

Qualitative evaluation of rock weir field performance and failure mechanisms

USGS Publications Warehouse

Mooney, David M.; Holmquist-Johnson, Christopher L.; Holburn, Elaina

2007-01-01

River spanning loose-rock structures provide sufficient head for irrigation diversion, permit fish passage over barriers, protect banks, stabilize degrading channels, activate side channels, reconnect floodplains, and create in-channel habitat. These structures are called by a variety of names including rock weirs, alphabet (U-, A-, V-, W-) weirs, Jhooks, and rock ramps. These structures share the common characteristics of:Loose rock construction materials (individually placed or dumped rocks with little or no concrete);Extents spanning the width of the river channel; andAn abrupt change in the water surface elevation at low flows.

Microfluidic channel-based wireless charging and communication platform for microsensors with miniaturized onboard antenna

NASA Astrophysics Data System (ADS)

Duan, G.; Zhao, X.; Seren, H. R.; Chen, C.; Li, A.; Zhang, X.

2016-12-01

A double layer spiral antenna with side length of 380 μm was fabricated by a multi-step electroplating process, and integrated with a commercialized passive RFID chip to realize the RF power harvesting and communication functions of a microsensor. To power up and communicate with the microchips, a single layer spiral reader antenna was fabricated on top of a glass substrate with side length of 1 mm. The microchips and the reader antenna were both optimized at the frequency of 915 MHz. Due to the small size of the reader antenna, the strength of the magnetic field decreased dramatically along the axial direction of the reader antenna, which limited the working distance to within 1 mm. To enclose the microchips within the reading range, a three-layer microfluidic channel was designed and fabricated. The channel and cover layers were fabricated by laser cutting of acrylic sheets, and bonded with the glass substrate to form the channel. To operate multiple microchips simultaneously, separation and focusing function units were also designed. Low loss pump oil was used to transport the microchips flowing inside the channel. Within the reading area, the microchips were powered up, and their ID information was retrieved and displayed on the computer interface successfully.

Intravascular Raman spectroscopic catheter for molecular diagnosis of atherosclerotic coronary disease

NASA Astrophysics Data System (ADS)

Komachi, Yuichi; Sato, Hidetoshi; Tashiro, Hideo

2006-10-01

An intravascular catheter for Raman spectroscopic detection and analysis of coronary atherosclerotic disease has been developed. The catheter, having an outer diameter of 2 mm, consisted of a side-view-type micro-Raman probe, an imaging fiber bundle, a working channel (injection drain), and a balloon. By inflating the balloon, the probe was brought close to the inner wall of a modeled blood flow system and detected a phantom target buried in the wall. Results obtained demonstrate the possibility of using the spectroscopic catheter for molecular diagnosis of coronary lesions.

A Security Proof of Measurement Device Independent Quantum Key Distribution: From the View of Information Theory

NASA Astrophysics Data System (ADS)

Li, Fang-Yi; Yin, Zhen-Qiang; Li, Hong-Wei; Chen, Wei; Wang, Shuang; Wen, Hao; Zhao, Yi-Bo; Han, Zheng-Fu

2014-07-01

Although some ideal quantum key distribution protocols have been proved to be secure, there have been some demonstrations that practical quantum key distribution implementations were hacked due to some real-life imperfections. Among these attacks, detector side channel attacks may be the most serious. Recently, a measurement device independent quantum key distribution protocol [Phys. Rev. Lett. 108 (2012) 130503] was proposed and all detector side channel attacks are removed in this scheme. Here a new security proof based on quantum information theory is given. The eavesdropper's information of the sifted key bits is bounded. Then with this bound, the final secure key bit rate can be obtained.

Deterministic MDI QKD with two secret bits per shared entangled pair

NASA Astrophysics Data System (ADS)

Zebboudj, Sofia; Omar, Mawloud

2018-03-01

Although quantum key distribution schemes have been proven theoretically secure, they are based on assumptions about the devices that are not yet satisfied with today's technology. The measurement-device-independent scheme has been proposed to shorten the gap between theory and practice by removing all detector side-channel attacks. On the other hand, two-way quantum key distribution schemes have been proposed to raise the secret key generation rate. In this paper, we propose a new quantum key distribution scheme able to achieve a relatively high secret key generation rate based on two-way quantum key distribution that also inherits the robustness of the measurement-device-independent scheme against detector side-channel attacks.

Reconfigurable Drive Current System

NASA Technical Reports Server (NTRS)

Alhorn, Dean C. (Inventor); Dutton, Kenneth R. (Inventor); Howard, David E. (Inventor); Smith, Dennis A. (Inventor)

2017-01-01

A reconfigurable drive current system includes drive stages, each of which includes a high-side transistor and a low-side transistor in a totem pole configuration. A current monitor is coupled to an output of each drive stage. Input channels are provided to receive input signals. A processor is coupled to the input channels and to each current monitor for generating at least one drive signal using at least one of the input signals and current measured by at least one of the current monitors. A pulse width modulation generator is coupled to the processor and each drive stage for varying the drive signals as a function of time prior to being supplied to at least one of the drive stages.

Insecurity of Detector-Device-Independent Quantum Key Distribution.

PubMed

Sajeed, Shihan; Huang, Anqi; Sun, Shihai; Xu, Feihu; Makarov, Vadim; Curty, Marcos

2016-12-16

Detector-device-independent quantum key distribution (DDI-QKD) held the promise of being robust to detector side channels, a major security loophole in quantum key distribution (QKD) implementations. In contrast to what has been claimed, however, we demonstrate that the security of DDI-QKD is not based on postselected entanglement, and we introduce various eavesdropping strategies that show that DDI-QKD is in fact insecure against detector side-channel attacks as well as against other attacks that exploit devices' imperfections of the receiver. Our attacks are valid even when the QKD apparatuses are built by the legitimate users of the system themselves, and thus, free of malicious modifications, which is a key assumption in DDI-QKD.

Joint digital signal processing for superchannel coherent optical communication systems.

PubMed

Liu, Cheng; Pan, Jie; Detwiler, Thomas; Stark, Andrew; Hsueh, Yu-Ting; Chang, Gee-Kung; Ralph, Stephen E

2013-04-08

Ultra-high-speed optical communication systems which can support ≥ 1Tb/s per channel transmission will soon be required to meet the increasing capacity demand. However, 1Tb/s over a single carrier requires either or both a high-level modulation format (i.e. 1024QAM) and a high baud rate. Alternatively, grouping a number of tightly spaced "sub-carriers" to form a terabit superchannel increases channel capacity while minimizing the need for high-level modulation formats and high baud rate, which may allow existing formats, baud rate and components to be exploited. In ideal Nyquist-WDM superchannel systems, optical subcarriers with rectangular spectra are tightly packed at a channel spacing equal to the baud rate, thus achieving the Nyquist bandwidth limit. However, in practical Nyquist-WDM systems, precise electrical or optical control of channel spectra is required to avoid strong inter-channel interference (ICI). Here, we propose and demonstrate a new "super receiver" architecture for practical Nyquist-WDM systems, which jointly detects and demodulates multiple channels simultaneously and mitigates the penalties associated with the limitations of generating ideal Nyquist-WDM spectra. Our receiver-side solution relaxes the filter requirements imposed on the transmitter. Two joint DSP algorithms are developed for linear ICI cancellation and joint carrier-phase recovery. Improved system performance is observed with both experimental and simulation data. Performance analysis under different system configurations is conducted to demonstrate the feasibility and robustness of the proposed joint DSP algorithms.

7. VIEW OF DAM 83, SHOWING DIVERSION GATES TO SOURIS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. VIEW OF DAM 83, SHOWING DIVERSION GATES TO SOURIS RIVER CHANNEL (LEFT) AND POND A (RIGHT) FROM THE WEST SIDE OF THE OUTLET CHANNEL, LOOKING SOUTHEAST (for view of the original diversion gate, see historic photograph, HAER No. ND-3-A-15) - Upper Souris National Wildlife Refuge, Dam 83, Souris River Basin, Foxholm, Surrey (England), ND

Torque limited drive for manual valves

DOEpatents

Elliott, Philip G.; Underwood, Daniel E.

1989-01-01

The present invention is directed to a torque-limiting handwheel device for preventing manual valves from being damaged due to the application of excessive torque during the opening or closing operation of the valves. Torque can only be applied when ridges in the handwheel assembly engage in channels machined in the face of the baseplate. The amount of torque required for disengagement of the ridges from the channels is determined by the force exerted by various Bellville springs and the inclination of the side faces of the channels.

Torque limited drive for manual valves

DOEpatents

Elliott, Philip G.; Underwood, Daniel E.

1989-06-06

The present invention is directed to a torque-limiting handwheel device for preventing manual valves from being damaged due to the application of excessive torque during the opening or closing operation of the valves. Torque can only be applied when ridges in the handwheel assembly engage in channels machined in the face of the baseplate. The amount of torque required for disengagement of the ridges from the channels is determined by the force exerted by various Bellville springs and the inclination of the side faces of the channels.

Guiding supersonic projectiles using optically generated air density channels

NASA Astrophysics Data System (ADS)

Johnson, Luke A.; Sprangle, Phillip

2015-09-01

We investigate the feasibility of using optically generated channels of reduced air density to provide trajectory correction (guiding) for a supersonic projectile. It is shown that the projectile experiences a force perpendicular to its direction of motion as one side of the projectile passes through a channel of reduced air density. A single channel of reduced air density can be generated by the energy deposited from filamentation of an intense laser pulse. We propose changing the laser pulse energy from shot-to-shot to build longer effective channels. Current femtosecond laser systems with multi-millijoule pulses could provide trajectory correction of several meters on 5 km trajectories for sub-kilogram projectiles traveling at Mach 3.

Multiple piece turbine blade

DOEpatents

Kimmel, Keith D [Jupiter, FL

2012-05-29

A turbine rotor blade with a spar and shell construction, the spar including an internal cooling supply channel extending from an inlet end on a root section and ending near the tip end, and a plurality of external cooling channels formed on both side of the spar, where a middle external cooling channel is connected to the internal cooling supply channels through a row of holes located at a middle section of the channels. The spar and the shell are held together by hooks that define serpentine flow passages for the cooling air and include an upper serpentine flow circuit and a lower serpentine flow circuit. the serpentine flow circuits all discharge into a leading edge passage or a trailing edge passage.

In silico Analysis of Conformational Changes Induced by Mutation of Aromatic Binding Residues: Consequences for Drug Binding in the hERG K+ Channel

PubMed Central

Knape, Kirsten; Linder, Tobias; Wolschann, Peter; Beyer, Anton; Stary-Weinzinger, Anna

2011-01-01

Pharmacological inhibition of cardiac hERG K+ channels is associated with increased risk of lethal arrhythmias. Many drugs reduce hERG current by directly binding to the channel, thereby blocking ion conduction. Mutation of two aromatic residues (F656 and Y652) substantially decreases the potency of numerous structurally diverse compounds. Nevertheless, some drugs are only weakly affected by mutation Y652A. In this study we utilize molecular dynamics simulations and docking studies to analyze the different effects of mutation Y652A on a selected number of hERG blockers. MD simulations reveal conformational changes in the binding site induced by mutation Y652A. Loss of π-π-stacking between the two aromatic residues induces a conformational change of the F656 side chain from a cavity facing to cavity lining orientation. Docking studies and MD simulations qualitatively reproduce the diverse experimentally observed modulatory effects of mutation Y652A and provide a new structural interpretation for the sensitivity differences. PMID:22194911

Identification and validation of midbrain Kcnq4 regulation of heavy alcohol consumption in rodents.

PubMed

McGuier, Natalie S; Rinker, Jennifer A; Cannady, Reginald; Fulmer, Diana B; Jones, Sara R; Hoffman, Michaela; Mulholland, Patrick J

2018-05-24

Currently available pharmacotherapies for treating alcohol use disorder (AUD) suffer from deleterious side effects and are not efficacious in diverse populations. Clinical and preclinical studies provide evidence that the Kcnq family of genes that encode K V 7 channels influence alcohol intake and dependence. K V 7 channels are a class of slowly activating voltage-dependent K + channels that regulate neuronal excitability. Studies indicate that the K V 7 channel positive modulator retigabine can decrease dopaminergic neuron firing, alter dopamine (DA) release, and reduce alcohol intake in heavy drinking rodents. Given the critical nature of ventral tegmental area (VTA) DA to the addiction process and predominant expression of Kcnq4 in DA neurons, we investigated the role of midbrain Kcnq genes and K V 7 channels in the VTA of genetically diverse mice and long-term heavy drinking rats, respectively. Integrative bioinformatics analysis identified negative correlations between midbrain Kcnq4 expression and alcohol intake and seeking behaviors. Kcnq4 expression levels were also correlated with dopaminergic-related phenotypes in BXD strains, and Kcnq4 was present in support intervals for alcohol sensitivity and alcohol withdrawal severity QTLs in rodents. Pharmacological validation studies revealed that VTA K V 7 channels regulate excessive alcohol intake in rats with a high-drinking phenotype. Administration of a novel and selective K V 7.2/4 channel positive modulator also reduced alcohol drinking in rats. Together, these findings indicate that midbrain Kcnq4 expression regulates alcohol-related behaviors in genetically diverse mice and provide evidence that K V 7.4 channels are a critical mediator of excessive alcohol drinking. Copyright © 2018 Elsevier Ltd. All rights reserved.

Channel Analysis for a 6.4 Gb s-1 DDR5 Data Buffer Receiver Front-End

NASA Astrophysics Data System (ADS)

Lehmann, Stefanie; Gerfers, Friedel

2017-09-01

In this contribution, the channel characteristic of the next generation DDR5-SDRAM architecture and possible approaches to overcome channel impairments are analysed. Because modern enterprise server applications and networks demand higher memory bandwidth, throughput and capacity, the DDR5-SDRAM specification is currently under development as a follow-up of DDR4-SDRAM technology. In this specification, the data rate is doubled to DDR5-6400 per IO as compared to the former DDR4-3200 architecture, resulting in a total per DIMM data rate of up to 409.6 Gb s-1. The single-ended multi-point-to-point CPU channel architecture in DDRX technology remains the same for DDR5 systems. At the specified target data rate, insertion loss, reflections, cross-talk as well as power supply noise become more severe and have to be considered. Using the data buffer receiver front-end of a load-reduced memory module, sophisticated equalisation techniques can be applied to ensure target BER at the increased data rate. In this work, the worst case CPU back-plane channel is analysed to derive requirements for receiver-side equalisation from the channel response characteristics. First, channel impairments such as inter-symbol-interference, reflections from the multi-point channel structure, and crosstalk from neighboring lines are analysed in detail. Based on these results, different correction methods for DDR5 data buffer front-ends are discussed. An architecture with 1-tap FFE in combination with a multi-tap DFE is proposed. Simulation of the architecture using a random input data stream is used to reveal the required DFE tap filter depth to effectively eliminate the dominant ISI and reflection based error components.

Kv11.1 (hERG)-induced cardiotoxicity: a molecular insight from a binding kinetics study of prototypical Kv11.1 (hERG) inhibitors

PubMed Central

Yu, Z; IJzerman, A P; Heitman, L H

2015-01-01

Background and Purpose Drug-induced arrhythmia due to blockade of the Kv11.1 channel (also known as the hERG K+ channel) is a frequent side effect. Previous studies have primarily focused on equilibrium parameters, i.e. affinity or potency, of drug candidates at the channel. The aim of this study was to determine the kinetics of the interaction with the channel for a number of known Kv11.1 blockers and to explore a possible correlation with the affinity or physicochemical properties of these compounds. Experimental Approach The affinity and kinetic parameters of 15 prototypical Kv11.1 inhibitors were evaluated in a number of [3H]-dofetilide binding assays. The lipophilicity (logKW-C8) and membrane partitioning (logKW-IAM) of these compounds were determined by means of HPLC analysis. Key Results A novel [3H]-dofetilide competition association assay was set up and validated, which allowed us to determine the binding kinetics of the Kv11.1 blockers used in this study. Interestingly, the compounds' affinities (Ki values) were correlated to their association rates rather than dissociation rates. Overall lipophilicity or membrane partitioning of the compounds were not correlated to their affinity or rate constants for the channel. Conclusions and Implications A compound's affinity for the Kv11.1 channel is determined by its rate of association with the channel, while overall lipophilicity and membrane affinity are not. In more general terms, our findings provide novel insights into the mechanism of action for a compound's activity at the Kv11.1 channel. This may help to elucidate how Kv11.1-induced cardiotoxicity is governed and how it can be circumvented in the future. PMID:25296617

Compounds having aromatic rings and side-chain amide-functionality and a method for transporting monovalent anions across biological membranes using the same

DOEpatents

Davis, Jeffery T [College Park, MD; Sidorov, Vladimir [Richmond, VA; Kotch, Frank W [New Phila., PA

2008-04-08

A compound containing at least two aromatic rings covalently bonded together, with each aromatic ring containing at least one oxyacetamide-based side chain, the compound being capable of forming a chloride ion channel across a lipid bilayer, and transporting chloride ion across the lipid bilayer.

Fine-scale relief related to late holocene channel shifting within the floor of the upper Redondo Fan, offshore Southern California

USGS Publications Warehouse

Normark, W.R.; Paull, C.K.; Caress, D.W.; Ussler, W.; Sliter, R.

2009-01-01

Erosional and depositional bedforms have been imaged at outcrop scale in the upper Redondo Fan, in the San Pedro Basin of offshore Southern California in ???600 m water depths, using an Autonomous Underwater Vehicle developed by the Monterey Bay Aquarium Research Institute. The Autonomous Underwater Vehicle is equipped with multibeam and chirp sub-bottom sonars. Sampling and photographic images using the Monterey Bay Aquarium Research Institute Remotely Operated Vehicle Tiburon provide groundtruth for the Autonomous Underwater Vehicle survey. The 0??3 m vertical and 1??5 m lateral bathymetric resolution and 0??1 m sub-bottom profile resolution provide unprecedented detail of bedform morphology and structure. Multiple channels within the Redondo Fan have been active at different times during the Late Holocene (0 to 3000 yr bp). The currently active channel extending from Redondo Canyon makes an abrupt 90?? turn at the canyon mouth before resuming a south-easterly course along the east side of the Redondo Fan. This channel is floored by sand and characterized by small steps generally <1 m in relief, spaced 10 to 80 m in the down-channel direction. A broader channel complex lies along the western side of the fan valley that was last active more than 850 years ago. Two distinct trains of large scours, with widths ranging from tens to a few hundred metres and depths of 20 m, occur on the floor of the western channel complex, which has a thin mud drape. If observed in cross-section only, these large scours would probably be misidentified as the thalweg of an active channel. ?? 2009 The Authors. Journal compilation ?? 2009 International Association of Sedimentologists.

Sedimentary environment and facies of St Lucia Estuary Mouth, Zululand, South Africa

NASA Astrophysics Data System (ADS)

Wright, C. I.; Mason, T. R.

The St. Lucia Estuary is situated on the subtropical, predominantly microtidal Zululand coast. Modern sedimentary environments within the estuary fall into three categories: (1) barrier environments; (2) abandoned channel environments; and (3) estuarine/lagoonal environments. The barrier-associated environment includes tidal inlet channel, inlet beach face, flood-tidal delta, ebb-tidal delta, spit, backspit and aeolian dune facies. The abandoned channel environment comprises washover fan, tidal creek tidal creek delta and back-barrier lagoon facies. The estuarine/lagoonal environment includes subtidal estuarine channel, side-attached bar, channel margin, mangrove fringe and channel island facies. Each sedimentary facies is characterised by sedimentary and biogenic structures, grain-size and sedimentary processes. Vertical facies sequences produced by inlet channel migration and lagoonal infilling are sufficiently distinct to be recognized in the geological record and are typical of a prograding shoreline.

Sediment Transport and Deposition Resulting from a Dam-Removal Sediment Pulse: Milltown Dam, Clark Fork River, MT

NASA Astrophysics Data System (ADS)

Wilcox, A. C.

2010-12-01

The removal of Milltown Dam in 2008 from the Clark Fork River, Montana, USA, lowered base level at the dam site by 9 m and triggered erosion of nearly 600,000 metric tons of predominantly fine reservoir sediment. Bedload and bed-material sampling, repeat topographic surveys, sediment transport modeling, geochemical fingerprinting of downstream sediments, and Lidar analysis have all been applied to study the upstream and downstream effects of the dam removal. In the years since dam breaching, successive years with similar peak flows (3-year recurrence interval) were followed by a third year with below-average runoff. Nearly all of the documented reservoir erosion occurred in the first year, when sand and silt was eroded and transported downstream. In subsequent years, minimal reservoir erosion occurred, in part as a result of active management to prevent further reservoir erosion, but coarse material eroded from the reservoir has dispersed downstream. Upstream responses in this system have been strongly mediated by Superfund remediation activities in Milltown Reservoir, in which over two million metric tons of contaminated sediments have been mechanically excavated. Downstream aggradation has been limited in the main channel but was initially substantial in bars and side channels of a multi-thread reach 21 to 25 km downstream of the dam site, suggesting that channel change has been influenced far more by the antecedent depositional environment than by proximity to the source of the sediment pulse. Comparison of observed erosion with pre-removal modeling shows that reservoir erosion exceeded model predictions by two orders of magnitude in the unconfined Clark Fork arm of the reservoir. In addition, fine reservoir sediments predicted to move exclusively in suspension traveled as bedload at lower transport stages. The resulting fine sediment deposition in substrate interstices, on bars, and in side channels of the gravel- and cobble-bed Clark Fork River is the most significant and lasting change to downstream geomorphic and ecological systems.

Pharmacovigilance database search discloses ClC-K channels as a novel target of the AT1 receptor blockers valsartan and olmesartan.

PubMed

Imbrici, Paola; Tricarico, Domenico; Mangiatordi, Giuseppe Felice; Nicolotti, Orazio; Lograno, Marcello Diego; Conte, Diana; Liantonio, Antonella

2017-07-01

Human ClC-K chloride channels are highly attractive targets for drug discovery as they have a variety of important physiological functions and are associated with genetic disorders. These channels are crucial in the kidney as they control chloride reabsorption and water diuresis. In addition, loss-of-function mutations of CLCNKB and BSND genes cause Bartter's syndrome (BS), whereas CLCNKA and CLCNKB gain-of-function polymorphisms predispose to a rare form of salt sensitive hypertension. Both disorders lack a personalized therapy that is in most cases only symptomatic. The aim of this study was to identify novel ClC-K ligands from drugs already on the market, by exploiting the pharmacological side activity of drug molecules available from the FDA Adverse Effects Reporting System database. We searched for drugs having a Bartter-like syndrome as a reported side effect, with the assumption that BS could be causatively related to the block of ClC-K channels. The ability of the selected BS-causing drugs to bind and block ClC-K channels was then validated through an integrated experimental and computational approach based on patch clamp electrophysiology in HEK293 cells and molecular docking simulations. Valsartan and olmesartan were able to block ClC-Ka channels and the molecular requirements for effective inhibition of these channels have been identified. These results suggest additional mechanisms of action for these sartans further to their primary AT 1 receptor antagonism and propose these compounds as leads for designing new potent ClC-K ligands. © 2017 The British Pharmacological Society.

The amiodarone derivative KB130015 activates hERG1 potassium channels via a novel mechanism

PubMed Central

Gessner, Guido; Macianskiene, Regina; Starkus, John G.; Schönherr, Roland; Heinemann, Stefan H.

2010-01-01

Human ether à go-go related gene (hERG1) potassium channels underlie the repolarizing IKr current in the heart. Since they are targets of various drugs with cardiac side effects we tested whether the amiodarone derivative 2-methyl-3-(3,5-diiodo-4-carboxymethoxybenzyl)benzofuran (KB130015) blocks hERG1 channels like its parent compound. Using patch-clamp and two-electrode voltage-clamp techniques we found that KB130015 blocks native and recombinant hERG1 channels at high voltages, but it activates them at low voltages. The activating effect has an apparent EC50 value of 12 μM and is brought about by an about 4-fold acceleration of activation kinetics and a shift in voltage-dependent activation by −16 mV. Channel activation was not use-dependent and was independent of inactivation gating. KB130015 presumably binds to the hERG1 pore from the cytosolic side and functionally competes with hERG1 block by amiodarone, E4031 (N-[4-[[1-[2-(6-methyl-2-pyridinyl)ethyl] -4-piperidinyl] carbonyl] phenyl] methanesulfonamide dihydrochloride), and sertindole. Vice versa, amiodarone attenuates hERG1 activation by KB130015. Based on synergic channel activation by mallotoxin and KB130015 we conclude that the hERG1 pore contains at least two sites for activators that are functionally coupled among each other and to the cavity-blocker site. KB130015 and amiodarone may serve as lead structures for the identification of hERG1 pore-interacting drugs favoring channel activation vs. block. PMID:20097192

Use of side-chain for rational design of n-type diketopyrrolopyrrole-based conjugated polymers: what did we find out?

PubMed

Kanimozhi, Catherine; Yaacobi-Gross, Nir; Burnett, Edmund K; Briseno, Alejandro L; Anthopoulos, Thomas D; Salzner, Ulrike; Patil, Satish

2014-08-28

The primary role of substituted side chains in organic semiconductors is to increase their solubility in common organic solvents. In the recent past, many literature reports have suggested that the side chains play a critical role in molecular packing and strongly impact the charge transport properties of conjugated polymers. In this work, we have investigated the influence of side-chains on the charge transport behavior of a novel class of diketopyrrolopyrrole (DPP) based alternating copolymers. To investigate the role of side-chains, we prepared four diketopyrrolopyrrole-diketopyrrolopyrrole (DPP-DPP) conjugated polymers with varied side-chains and carried out a systematic study of thin film microstructure and charge transport properties in polymer thin-film transistors (PTFTs). Combining results obtained from grazing incidence X-ray diffraction (GIXD) and charge transport properties in PTFTs, we conclude side-chains have a strong influence on molecular packing, thin film microstructure, and the charge carrier mobility of DPP-DPP copolymers. However, the influence of side-chains on optical properties was moderate. The preferential "edge-on" packing and dominant n-channel behavior with exceptionally high field-effect electron mobility values of >1 cm(2) V(-1) s(-1) were observed by incorporating hydrophilic (triethylene glycol) and hydrophobic side-chains of alternate DPP units. In contrast, moderate electron and hole mobilities were observed by incorporation of branched hydrophobic side-chains. This work clearly demonstrates that the subtle balance between hydrophobicity and hydrophilicity induced by side-chains is a powerful strategy to alter the molecular packing and improve the ambipolar charge transport properties in DPP-DPP based conjugated polymers. Theoretical analysis supports the conclusion that the side-chains influence polymer properties through morphology changes, as there is no effect on the electronic properties in the gas phase. The exceptional electron mobility is at least partially a result of the strong intramolecular conjugation of the donor and acceptor as evidenced by the unusually wide conduction band of the polymer.

CFD analyses of coolant channel flowfields

NASA Technical Reports Server (NTRS)

Yagley, J. A.; Feng, J.; Merkle, Charles L.

1993-01-01

The flowfield characteristics in a rocket engine coolant channels are analyzed by means of a numerical model. The channels are characterized by large length to diameter ratios, high Reynolds numbers, and asymmetrical heating. At representative flow conditions, the channel length is approximately twice the hydraulic entrance length so fully developed conditions are reached. The supercritical hydrogen coolant introduces strong property variations that have a major influence on the developing flow and the resulting heat transfer. Comparisons of constant and variable property solutions show substantial differences. The density variation accelerates the fluid in the channels increasing the pressure drop without an accompanying increase in heat flux. Analyses of the inlet configuration suggest that side entry from a manifold can affect the development of the velocity profile because of vortices generated as the flow enters the channel.

A General Computational Approach for Magnetohydrodynamic Flows Using the CFX Code: Buoyant Flow Through a Vertical Square Channel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Di Piazza, Ivan; Buehler, Leo

2000-09-15

The buoyancy-driven magnetoconvection in the cross section of an infinitely long vertical square duct is investigated numerically using the CFX code package. The implementation of a magnetohydrodynamic (MHD) problem in CFX is discussed, with particular reference to the Lorentz forces and the electric potential boundary conditions for arbitrary electrical conductivity of the walls. The method proposed is general and applies to arbitrary geometries with an arbitrary orientation of the magnetic field. Results for fully developed flow under various thermal boundary conditions are compared with asymptotic analytical solutions. The comparison shows that the asymptotic analysis is confirmed for highly conducting wallsmore » as high velocity jets occur at the side walls. For weakly conducting walls, the side layers become more conducting than the side walls, and strong electric currents flow within these layers parallel to the magnetic field. As a consequence, the velocity jets are suppressed, and the core solution is only corrected by the viscous forces near the wall. The implementation of MHD in CFX is achieved.« less

Experimental investigation on the heat transfer characteristics and flow pattern in vertical narrow channels heated from one side

NASA Astrophysics Data System (ADS)

Huang, Lihao; Li, Gang; Tao, Leren

2016-07-01

Experimental investigation for the flow boiling of water in a vertical rectangular channel was conducted to reveal the boiling heat transfer mechanism and flow patterns map aspects. The onset of nucleate boiling went upward with the increasing of the working fluid mass flow rate or the decreasing of the inlet working fluid temperature. As the vapour quality was increased, the local heat transfer coefficient increased first, then decreased, followed by various flow patterns. The test data from other researchers had a similar pattern transition for the bubble-slug flow and the slug-annular flow. Flow pattern transition model analysis was performed to make the comparison with current test data. The slug-annular and churn-annular transition models showed a close trend with current data except that the vapor phase superficial velocity of flow pattern transition was much higher than that of experimental data.

Sample pre-concentration with high enrichment factors at a fixed location in paper-based microfluidic devices.

PubMed

Yeh, Shih-Hao; Chou, Kuang-Hua; Yang, Ruey-Jen

2016-03-07

The lack of sensitivity is a major problem among microfluidic paper-based analytical devices (μPADs) for early disease detection and diagnosis. Accordingly, the present study presents a method for improving the enrichment factor of low-concentration biomarkers by using shallow paper-based channels realized through a double-sided wax-printing process. In addition, the enrichment factor is further enhanced by exploiting the ion concentration polarization (ICP) effect on the cathodic side of the nanoporous membrane, in which a stationary sample plug is obtained. The occurrence of ICP on the shallow-channel μPAD is confirmed by measuring the current-voltage response as the external voltage is increased from 0 to 210 V (or the field strength from 0 to 1.05 × 10(4) V m(-1)) over 600 s. In addition, to the best of our knowledge, the electroosmotic flow (EOF) speed on the μPAD fabricated with a wax-channel is measured for the first time using a current monitoring method. The experimental results show that for a fluorescein sample, the concentration factor is increased from 130-fold in a conventional full-thickness paper channel to 944-fold in the proposed shallow channel. Furthermore, for a fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) sample, the proposed shallow-channel μPAD achieves an 835-fold improvement in the concentration factor. The concentration technique presented here provides a novel strategy for enhancing the detection sensitivity of μPAD applications.

Topographical localization of the C-terminal region of the voltage-dependent sodium channel from Electrophorus electricus using antibodies raised against a synthetic peptide

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gordon, R.D.; Fieles, W.E.; Schotland, D.L.

1987-01-01

A peptide corresponding to amino acid residues 1783-1794 near the C terminus of the electric eel sodium channel primary sequence of the eel (Electrophorus electricus) sodium channel has been synthesized and used to raise an antiserum in rabbits. This antiserum specifically recognized the peptide in a solid-phase radioimmunoassay. Specificity of the antiserum for the native channel protein was shown by its specific binding to a 280-kDa protein in immunoblots of eel electroplax membrane proteins. The antiserum also specifically labeled the innervated membrane of the eel electroplax in immunofluorescent studies. The membrane topology of the peptide recognized by this antiserum wasmore » proved in binding studies using oriented electroplax membrane vesicles. These vesicles were 98% right-side-out as determined by (/sup 3/H)saxitoxin binding. Binding of the antipeptide antiserum to this fraction was measured before and after permeabilization with 0.01% saponin. Specific binding to intact vesicles was low, but this binding increased 10-fold after permeabilization, implying a cytoplasmic orientation for the peptide. Confirmation for this orientation was then sought by localizing the antibody bound to intact electroplax cells with immunogold electron microscopy. The data imply that the region of the sodium channel primary sequence near the C terminus that is recognized by the anitserum is localized on the cytoplasmic side of the membrane; this localization provides some further constraints on models of sodium channel tertiary structure.« less

Lazy river on Mars: Ring-shaped fluvial channel discovered north of Capri Chasma

NASA Astrophysics Data System (ADS)

Thomson, B. J.

2017-12-01

Many features on Mars are strange, but some are stranger than others. Fluvial features on Mars come in several basic flavors: branching valley networks, massive outflow channels, and possibly presently active recurring slope lineae. Here, we identify a small, valley network-like channel segment whose planform appearance traces out a nearly complete circle. One of the key tenants of hydrogeology and plumbing is that "stuff flows downhill." A seemingly circular loop implies a gross violation of the downhill flow rule, akin to a visual claim of perpetual motion. This M.C. Escher-inspired landform is located at 6.45°S, 39.70°W inside Innsbruck crater, a 59-km diameter impact structure that is just north of Capri Chasma. A close inspection reveals that the loop is not 100% continuous; there is a slight break on the western side of the loop. The pair of channels on either side of this gap terminate abruptly. These appear to be points of origin rather than termini, although admittedly the direction(s) of flow within the channel segments are difficult to constrain uniquely. The overall morphology of this near-circular channel system implies a local source limited both in duration and volume. Assuming that the fluid involved was water, the volume of water was sufficient for incipient erosion of the terrain, but not sufficient to have ponded or continued to flow. Here, the combined infiltration and evaporation rates must have been sufficiently large such that a breakout flow did not occur.

Synchrotron X-ray studies of the keel of the short-spined sea urchin Lytechinus variegatus: absorption microtomography (microCT) and small beam diffraction mapping.

PubMed

Stock, S R; Barss, J; Dahl, T; Veis, A; Almer, J D; Carlo, F

2003-05-01

In sea urchin teeth, the keel plays an important structural role, and this paper reports results of microstructural characterization of the keel of Lytechinus variegatus using two noninvasive synchrotron x-ray techniques: x-ray absorption microtomography (microCT) and x-ray diffraction mapping. MicroCT with 14 keV x-rays mapped the spatial distribution of mineral at the 1.3 microm level in a millimeter-sized fragment of a mature portion of the keel. Two rows of low absorption channels (i.e., primary channels) slightly less than 10 microm in diameter were found running linearly from the flange to the base of the keel and parallel to its sides. The primary channels paralleled the oral edge of the keel, and the microCT slices revealed a planar secondary channel leading from each primary channel to the side of the keel. The primary and secondary channels were more or less coplanar and may correspond to the soft tissue between plates of the carinar process. Transmission x-ray diffraction with 80.8 keV x-rays and a 0.1 mm beam mapped the distribution of calcite crystal orientations and the composition Ca(1-x)Mg(x)CO(3) of the calcite. Unlike the variable Mg concentration and highly curved prisms found in the keel of Paracentrotus lividus, a constant Mg content (x = 0.13) and relatively little prism curvature was found in the keel of Lytechinus variegatus.

Laser-assisted patterning of double-sided adhesive tapes for optofluidic chip integration

NASA Astrophysics Data System (ADS)

Zamora, Vanessa; Janeczka, Christian; Arndt-Staufenbiel, Norbert; Havlik, George; Queisser, Marco; Schröder, Henning

2018-02-01

Portable high-sensitivity biosensors exhibit a growing demand in healthcare, food industry and environmental monitoring sectors. Optical biosensors based on photonic integration platforms are attractive candidates due to their high sensitivity, compactness and multiplexing capabilities. However, they need a low-cost and reliable integration with the microfluidic system. Laser-micropatterned double-sided biocompatible adhesive tapes are promising bonding layers for hybrid integration of an optofluidic biochip. As a part of the EU-PHOCNOSIS project, double-sided adhesive tapes have been proposed to integrate the polymer microfluidic system with the optical integrated waveguide sensor chip. Here the adhesive tape should be patterned in a micrometer scale in order to create an interaction between the sample that flows through the polymer microchannel and the photonic sensing microstructure. Three laser-assisted structuring methods are investigated to transfer microchannel patterns to the adhesive tape. The test structure design consists of a single channel with 400 μm wide, 30 mm length and two circular receivers with 3 mm radius. The best structuring results are found by using the picosecond UV laser where smooth and straight channel cross-sections are obtained. Such patterned tapes are used to bond blank polymer substrates to blank silicon substrates. As a proof of concept, the hybrid integration is tested using colored DI-water. Structuring tests related to the reduction of channel widths are also considered in this work. The use of this technique enables a simple and rapid manufacturing of narrow channels (50-60 μm in width) in adhesive tapes, achieving a cheap and stable integration of the optofluidic biochip.

76 FR 43685 - Designation of an Ocean Dredged Material Disposal Site (ODMDS) in the Gulf of Mexico Off the...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-21

... Atchafalaya River and the Atchafalaya River Bar Channel (ARBC), located within the Federally-authorized and... Mexico. The ARBC is located in an area of heavy sedimentation. The bed load fraction of the sediment...) ODMDS on the east side of the channel (the ODMDS-East). Concern has been expressed, and Corps studies...

Research and development of asymmetrical heat transfer augmentation method in radial channels of blades for high temperature gas turbines

NASA Astrophysics Data System (ADS)

Shevchenko, I. V.; Rogalev, A. N.; Garanin, I. V.; Vegera, A. N.; Kindra, V. O.

2017-11-01

The serpentine-like one and half-pass cooling channel systems are primarily used in blades fabricated by the lost-wax casting process. The heat transfer turbulators like cross-sectional or angled ribs used in channels of the midchord region failed to eliminate the temperature irregularity from the suction and pressure sides, which is reaching 200°C for a first stage blade of the high-pressure turbine for an aircraft engine. This paper presents the results of a numerical and experimental test of an advanced heat transfer augmentation system in radial channels developed for alignment of the temperature field from the suction and pressure sides. A numerical simulation of three-dimensional coolant flow for a wide range of Reynolds numbers was carried out using ANSYS CFX software. Effect of geometrical parameters on the heat removal asymmetry was determined. The test results of a blade with the proposed intensification system conducted in a liquid-metal thermostat confirmed the accuracy of calculations. Based on the experimental data, the dependencies for calculation of heat transfer coefficients to the cooling air in the blade studied were obtained.

Transfer of metabolites across the peroxisomal membrane.

PubMed

Antonenkov, Vasily D; Hiltunen, J Kalervo

2012-09-01

Peroxisomes perform a large variety of metabolic functions that require a constant flow of metabolites across the membranes of these organelles. Over the last few years it has become clear that the transport machinery of the peroxisomal membrane is a unique biological entity since it includes nonselective channels conducting small solutes side by side with transporters for 'bulky' solutes such as ATP. Electrophysiological experiments revealed several channel-forming activities in preparations of plant, mammalian, and yeast peroxisomes and in glycosomes of Trypanosoma brucei. The properties of the first discovered peroxisomal membrane channel - mammalian Pxmp2 protein - have also been characterized. The channels are apparently involved in the formation of peroxisomal shuttle systems and in the transmembrane transfer of various water-soluble metabolites including products of peroxisomal β-oxidation. These products are processed by a large set of peroxisomal enzymes including carnitine acyltransferases, enzymes involved in the synthesis of ketone bodies, thioesterases, and others. This review discusses recent data pertaining to solute permeability and metabolite transport systems in peroxisomal membranes and also addresses mechanisms responsible for the transfer of ATP and cofactors such as an ATP transporter and nudix hydrolases. Copyright © 2012 Elsevier B.V. All rights reserved.

Gas block mechanism for water removal in fuel cells

DOEpatents

Issacci, Farrokh; Rehg, Timothy J.

2004-02-03

The present invention is directed to apparatus and method for cathode-side disposal of water in an electrochemical fuel cell. There is a cathode plate. Within a surface of the plate is a flow field comprised of interdigitated channels. During operation of the fuel cell, cathode gas flows by convection through a gas diffusion layer above the flow field. Positioned at points adjacent to the flow field are one or more porous gas block mediums that have pores sized such that water is sipped off to the outside of the flow field by capillary flow and cathode gas is blocked from flowing through the medium. On the other surface of the plate is a channel in fluid communication with each porous gas block mediums. The method for water disposal in a fuel cell comprises installing the cathode plate assemblies at the cathode sides of the stack of fuel cells and manifolding the single water channel of each of the cathode plate assemblies to the coolant flow that feeds coolant plates in the stack.

Apparatus for sensing patterns of electrical field variations across a surface

DOE Office of Scientific and Technical Information (OSTI.GOV)

Warren, William L.; Devine, Roderick A. B.

An array of nonvolatile field effect transistors used to sense electric potential variations. The transistors owe their nonvolatility to the movement of protons within the oxide layer that occurs only in response to an externally applied electric potential between the gate on one side of the oxide and the source/drain on the other side. The position of the protons within the oxide layer either creates or destroys a conducting channel in the adjacent source/channel/drain layer below it, the current in the channel being measured as the state of the nonvolatile memory. The protons can also be moved by potentials createdmore » by other instrumentalities, such as charges on fingerprints or styluses above the gates, pressure on a piezoelectric layer above the gates, light shining upon a photoconductive layer above the gates. The invention allows sensing of fingerprints, handwriting, and optical images, which are converted into digitized images thereof in a nonvolatile format.« less

Laser Anemometer Measurements of the Flow Field in a 4:1 Pressure Ratio Centrifugal Impeller

NASA Technical Reports Server (NTRS)

Skoch, G. J.; Prahst, P. S.; Wernet, M. P.; Wood, J. R.; Strazisar, A. J.

1997-01-01

A laser-doppler anemometer was used to obtain flow-field velocity measurements in a 4:1 pressure ratio, 4.54 kg/s (10 lbm/s), centrifugal impeller, with splitter blades and backsweep, which was configured with a vaneless diffuser. Measured through-flow velocities are reported for ten quasi-orthogonal survey planes at locations ranging from 1% to 99% of main blade chord. Measured through-flow velocities are compared to those predicted by a 3-D viscous steady flow analysis (Dawes) code. The measurements show the development and progression through the impeller and vaneless diffuser of a through-flow velocity deficit which results from the tip clearance flow and accumulation of low momentum fluid centrifuged from the blade and hub surfaces. Flow traces from the CFD analysis show the origin of this deficit which begins to grow in the inlet region of the impeller where it is first detected near the suction surface side of the passage. It then moves toward the pressure side of the channel, due to the movement of tip clearance flow across the impeller passage, where it is cut by the splitter blade leading edge. As blade loading increases toward the rear of the channel the deficit region is driven back toward the suction surface by the cross-passage pressure gradient. There is no evidence of a large wake region that might result from flow separation and the impeller efficiency is relatively high. The flow field in this impeller is quite similar to that documented previously by NASA Lewis in a large low-speed backswept impeller.

Validation of the Measurement of Pollution in the Troposphere (MOPITT) Experiment by Ground-Based Infrared Solar Spectroscopic Measurements of Carbon Monoxide (CO) and Methane (CH4)

NASA Technical Reports Server (NTRS)

Pougatchev, Nikita

2003-01-01

The goal of the MOPITT experiment is to enhance our knowledge of the lower atmosphere system and particularly how it interacts with the surface/ocean/biomass systems. The particular focus is the distribution, transport, sources and sinks of carbon monoxide and methane in the troposphere. The MOPITT instrument was launched on EOS TERRA satellite December 18, 1999. After the launch and until March 22, 2000 the MOPITT instrument was in engineering and calibration mode. Beginning March 23, 2000 through May 6, 2001 the instrument was in a science measurement mode with some calibration breaks. On May 7, 2001 a criocooler on a side B died and channels 1 - 4 became inoperational. The MOPITT resumed its scientific measurements on August 25, 2001 with channels 5 - 8. With some calibration breaks the instrument currently provides the data. The project has three elements to it: hardware, data analysis and modeling. The MOPITT instrument, on the NASA EOS Terra satellite, measures the upwelling infrared radiance. Using the technique of correlation spectroscopy, information regarding the distribution of atmospheric CO and CH4 can be extracted. By using appropriate data analysis techniques, concentration profiles of CO are currently obtained on a global basis at a reasonably high horizontal (approximately 22km) and vertical resolution (approximately 3km). Column amounts of methane will be derived over the sunlit side of the orbit. These profiles are assimilated into models to study the chemistry and dynamics of CO, CH4 and other constituents of the lower atmosphere.

State-dependent block of CNG channels by dequalinium.

PubMed

Rosenbaum, Tamara; Gordon-Shaag, Ariela; Islas, León D; Cooper, Jeremy; Munari, Mika; Gordon, Sharona E

2004-03-01

Cyclic nucleotide-gated (CNG) ion channels are nonselective cation channels with a high permeability for Ca(2+). Not surprisingly, they are blocked by a number of Ca(2+) channel blockers including tetracaine, pimozide, and diltiazem. We studied the effects of dequalinium, an extracellular blocker of the small conductance Ca(2+)-activated K(+) channel. We previously noted that dequalinium is a high-affinity blocker of CNGA1 channels from the intracellular side, with little or no state dependence at 0 mV. Here we examined block by dequalinium at a broad range of voltages in both CNGA1 and CNGA2 channels. We found that dequalinium block was mildly state dependent for both channels, with the affinity for closed channels 3-5 times higher than that for open channels. Mutations in the S4-S5 linker did not alter the affinity of open channels for dequalinium, but increased the affinity of closed channels by 10-20-fold. The state-specific effect of these mutations raises the question of whether/how the S4-S5 linker alters the binding of a blocker within the ion permeation pathway.

Implementation of continuous-variable quantum key distribution with composable and one-sided-device-independent security against coherent attacks.

PubMed

Gehring, Tobias; Händchen, Vitus; Duhme, Jörg; Furrer, Fabian; Franz, Torsten; Pacher, Christoph; Werner, Reinhard F; Schnabel, Roman

2015-10-30

Secret communication over public channels is one of the central pillars of a modern information society. Using quantum key distribution this is achieved without relying on the hardness of mathematical problems, which might be compromised by improved algorithms or by future quantum computers. State-of-the-art quantum key distribution requires composable security against coherent attacks for a finite number of distributed quantum states as well as robustness against implementation side channels. Here we present an implementation of continuous-variable quantum key distribution satisfying these requirements. Our implementation is based on the distribution of continuous-variable Einstein-Podolsky-Rosen entangled light. It is one-sided device independent, which means the security of the generated key is independent of any memoryfree attacks on the remote detector. Since continuous-variable encoding is compatible with conventional optical communication technology, our work is a step towards practical implementations of quantum key distribution with state-of-the-art security based solely on telecom components.

Implementation of continuous-variable quantum key distribution with composable and one-sided-device-independent security against coherent attacks

PubMed Central

Gehring, Tobias; Händchen, Vitus; Duhme, Jörg; Furrer, Fabian; Franz, Torsten; Pacher, Christoph; Werner, Reinhard F.; Schnabel, Roman

2015-01-01

Secret communication over public channels is one of the central pillars of a modern information society. Using quantum key distribution this is achieved without relying on the hardness of mathematical problems, which might be compromised by improved algorithms or by future quantum computers. State-of-the-art quantum key distribution requires composable security against coherent attacks for a finite number of distributed quantum states as well as robustness against implementation side channels. Here we present an implementation of continuous-variable quantum key distribution satisfying these requirements. Our implementation is based on the distribution of continuous-variable Einstein–Podolsky–Rosen entangled light. It is one-sided device independent, which means the security of the generated key is independent of any memoryfree attacks on the remote detector. Since continuous-variable encoding is compatible with conventional optical communication technology, our work is a step towards practical implementations of quantum key distribution with state-of-the-art security based solely on telecom components. PMID:26514280

A novel wavelength multiplexer/demutiplexer based on side-port multimode interference coupler

NASA Astrophysics Data System (ADS)

Wei, Shile; Jian, Wu; Zhao, Lingjuan; Qiu, Jifang; Yin, Zuoshan; Hui, Rongqing

2014-05-01

Based on side-port multimode interference coupler, a novel design of 1.31/1.55-μm wavelength multiplexer/demutiplexer on SOI platform with conventional channel waveguides is proposed and analyzed by using wide-angle beam propagation method. With a 25.9μm long ultra-short MMI section, nearly an order of magnitude shorter than that of the previously reported 1.31/1.55-μm wavelength MMI splitters on SOI, simulation results exhibit contrasts of 28dB and 25dB at wavelength 1.31 and 1.55 μm, respectively, and the insertion losses are both below 0.55dB. Meanwhile, the analysis shows that the proposed structure has larger fabrication tolerances than restricted MMI based structures and the present design methodology also applies to split other wavelengths and in different material platforms, such as InP, GaAs and PLC guides, etc.

Resonant electron capture by aspartame and aspartic acid molecules.

PubMed

Muftakhov, M V; Shchukin, P V

2016-12-30

The processes for dissociative electron capture are the key mechanisms for decomposition of biomolecules, proteins in particular, under interaction with low-energy electrons. Molecules of aspartic acid and aspartame, i.e. modified dipeptides, were studied herein to define the impact of the side functional groups on peptide chain decomposition in resonant electron-molecular reactions. The processes of formation and decomposition of negative ions of both aspartame and aspartic acid were studied by mass spectrometry of negative ions under resonant electron capture. The obtained mass spectra were interpreted under thermochemical analysis by quantum chemical calculations. Main channels of negative molecular ions fragmentation were found and characteristic fragment ions were identified. The СООН fragment of the side chain in aspartic acid is shown to play a key role like the carboxyl group in amino acids and aliphatic oligopeptides. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

Duct flow nonuniformities for Space Shuttle Main Engine (SSME)

NASA Technical Reports Server (NTRS)

1988-01-01

Analytical capabilities for modeling hot gas flow on the fuel side of the Space Shuttle Main Engines are developed. Emphasis is placed on construction and documentation of a computational grid code for modeling an elliptical two-duct version of the fuel side hot gas manifold. Computational results for flow past a support strut in an annular channel are also presented.

Stable wafer-carrier system

DOEpatents

Rozenzon, Yan; Trujillo, Robert T; Beese, Steven C

2013-10-22

One embodiment of the present invention provides a wafer-carrier system used in a deposition chamber for carrying wafers. The wafer-carrier system includes a base susceptor and a top susceptor nested inside the base susceptor with its wafer-mounting side facing the base susceptor's wafer-mounting side, thereby forming a substantially enclosed narrow channel. The base susceptor provides an upward support to the top susceptor.

Photovoltaic Roofs

NASA Technical Reports Server (NTRS)

Drummond, R. W., Jr.; Shepard, N. F., Jr.

1984-01-01

Solar cells perform two functions: waterproofing roof and generating electricity. Sections through horizontal and slanting joints show overlapping modules sealed by L-section rubber strips and side-by-side modules sealed by P-section strips. Water seeping through seals of slanting joints drains along channels. Rooftop photovoltaic array used watertight south facing roof, replacing shingles, tar, and gravel. Concept reduces cost of residential solar-cell array.

5. Downstream elevation, view to southeast. Dark stains on side ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Downstream elevation, view to southeast. Dark stains on side of main girder are from deck drain scuppers, marking deck level within the girders. Compare this view and CA-126-7 to CA-126-19 for indication of severity of siltation of Salt River channel has silted. - Salt River Bridge, Spanning Salt River at Dillon Road, Ferndale, Humboldt County, CA

Quantum key distribution with untrusted detectors

NASA Astrophysics Data System (ADS)

González, P.; Rebón, L.; Ferreira da Silva, T.; Figueroa, M.; Saavedra, C.; Curty, M.; Lima, G.; Xavier, G. B.; Nogueira, W. A. T.

2015-08-01

Side-channel attacks currently constitute the main challenge for quantum key distribution (QKD) to bridge theory with practice. So far two main approaches have been introduced to address this problem, (full) device-independent QKD and measurement-device-independent QKD. Here we present a third solution that might exceed the performance and practicality of the previous two in circumventing detector side-channel attacks, which arguably is the most hazardous part of QKD implementations. Our proposal has, however, one main requirement: the legitimate users of the system need to ensure that their labs do not leak any unwanted information to the outside. The security in the low-loss regime is guaranteed, while in the high-loss regime we already prove its robustness against some eavesdropping strategies.

Breakdown flash at telecom wavelengths in InGaAs avalanche photodiodes

NASA Astrophysics Data System (ADS)

Shi, Yicheng; Lim, Janet Zheng Jie; Poh, Hou Shun; Tan, Peng Kian; Tan, Peiyu Amelia; Ling, Alexander; Kurtsiefer, Christian

2017-11-01

Quantum key distribution (QKD) at telecom wavelengths (1260-1625nm) has the potential for fast deployment due to existing optical fibre infrastructure and mature telecom technologies. At these wavelengths, indium gallium arsenide (InGaAs) avalanche photodiode (APD) based detectors are the preferred choice for photon detection. Similar to their silicon counterparts used at shorter wavelengths, they exhibit fluorescence from recombination of electron-hole pairs generated in the avalanche breakdown process. This fluorescence may open side channels for attacks on QKD systems. Here, we characterize the breakdown fluorescence from two commercial InGaAs single photon counting modules, and find a spectral distribution between 1000nm and 1600nm. We also show that by spectral filtering, this side channel can be efficiently suppressed.

Breakdown flash at telecom wavelengths in InGaAs avalanche photodiodes.

PubMed

Shi, Yicheng; Lim, Janet Zheng Jie; Poh, Hou Shun; Tan, Peng Kian; Tan, Peiyu Amelia; Ling, Alexander; Kurtsiefer, Christian

2017-11-27

Quantum key distribution (QKD) at telecom wavelengths (1260 - 1625 nm) has the potential for fast deployment due to existing optical fibre infrastructure and mature telecom technologies. At these wavelengths, Indium Gallium Arsenide (InGaAs) avalanche photodiode (APD) based detectors are the preferred choice for photon detection. Similar to their Silicon counterparts used at shorter wavelengths, they exhibit fluorescence from recombination of electron-hole pairs generated in the avalanche breakdown process. This fluorescence may open side channels for attacks on QKD systems. Here, we characterize the breakdown fluorescence from two commercial InGaAs single photon counting modules, and find a spectral distribution between 1000 nm and 1600 nm. We also show that by spectral filtering, this side channel can be efficiently suppressed.

Water jacket for solid particle solar receiver

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wasyluk, David T.

A solar receiver includes: water jacket panels each having a light-receiving side and a back side with a watertight sealed plenum defined in-between; light apertures passing through the watertight sealed plenums to receive light from the light-receiving sides of the water jacket panels; a heat transfer medium gap defined between the back sides of the water jacket panels and a cylindrical back plate; and light channeling tubes optically coupled with the light apertures and extending into the heat transfer medium gap. In some embodiments ends of the light apertures at the light receiving side of the water jacket panel aremore » welded together to define at least a portion of the light-receiving side. A cylindrical solar receiver may be constructed using a plurality of such water jacket panels arranged with their light-receiving sides facing outward.« less

Single channel double-duct liquid metal electrical generator using a magnetohydrodynamic device

DOEpatents

Haaland, C.M.; Deeds, W.E.

1999-07-13

A single channel double-duct liquid metal electrical generator using a magnetohydrodynamic (MHD) device. The single channel device provides useful output AC electric energy. The generator includes a two-cylinder linear-piston engine which drives liquid metal in a single channel looped around one side of the MHD device to form a double-duct contra-flowing liquid metal MHD generator. A flow conduit network and drive mechanism are provided for moving liquid metal with an oscillating flow through a static magnetic field to produce useful AC electric energy at practical voltages and currents. Variable stroke is obtained by controlling the quantity of liquid metal in the channel. High efficiency is obtained over a wide range of frequency and power output. 5 figs.

Single channel double-duct liquid metal electrical generator using a magnetohydrodynamic device

DOEpatents

Haaland, Carsten M.; Deeds, W. Edward

1999-01-01

A single channel double-duct liquid metal electrical generator using a magnetohydrodynamic (MHD) device. The single channel device provides useful output AC electric energy. The generator includes a two-cylinder linear-piston engine which drives liquid metal in a single channel looped around one side of the MHD device to form a double-duct contra-flowing liquid metal MHD generator. A flow conduit network and drive mechanism are provided for moving liquid metal with an oscillating flow through a static magnetic field to produce useful AC electric energy at practical voltages and currents. Variable stroke is obtained by controlling the quantity of liquid metal in the channel. High efficiency is obtained over a wide range of frequency and power output.

Using Braid Plain Ecology and Geomorphology to Inform Bank Erosion Management along a Braided River, Matanuska River, Alaska

NASA Astrophysics Data System (ADS)

Curran, J. H.; McTeague, M. L.

2010-12-01

Braided rivers are inherently dynamic but quantifying the nature and implications of this dynamism can contribute to more comprehensive understanding of these systems and management of the river corridor. Bank erosion along the glacial, braided Matanuska River in southcentral Alaska has challenged generations of officials and generated a host of proposed solutions such as riprapped banks, dikes, gravel mining, and trenching. Increasingly, assessment of the technical feasibility of these methods has been accompanied by consideration of ecological factors and nonstructural solutions. The Matanuska River is braided over 85 percent of its course and clearwater side channels in abandoned braid plain areas provide as much as 90 percent of the spawning habitat in the basin for chum and sockeye salmon (Oncorhynchus keta and O. nerka). An assessment of braid plain vegetation, bank erosion rates, effects of a large flood, and distribution of clearwater side channels establishes a scientific basis for ecological and geomorphological considerations and recently helped guide development of a management plan for the river corridor. A historical analysis of braid plain features, marginal positions, and vegetation patterns from 1949, 1962, and 2006 orthophotographs showed that the 2006 braid plain was 43 percent vegetated and had an average age of 16 years. Only about 4 percent of the braid plain contained vegetated islands and over 60 percent of these were young and sparsely vegetated, implying that a suite of active channels migrated frequently across the braid plain and that vegetation did not appreciably limit channel movement. Rates of erosion to the braid plain margins averaged 0.3 m/yr from 1949 to 2006 but erosion was localized, with 64 percent of the erosion at only 8 percent of the banks. Cumulative bank change was twice as great along banks consisting of Holocene fluvial deposits (fans and terraces) identified during Geographic Information System (GIS) mapping than on other features. River-long erosion rates were twice as great for 1949-62 than for 1962-2006, despite a flood with a less than 0.002 percent exceedance probability in 1971 and slightly higher average peak flood magnitudes in the latter period. Of the 20 areas with erosion greater than 70 m from 1949-2006, only 9 were eroded in both periods and only one had detectable erosion in the sub-period from 2004 to 2006. This disconnect of erosion with flooding and the variable timing of historical erosion suggests that erosion was sporadic and more related to the presence of the river against the bank and bank erodibility than to more readily monitored variables. Clearwater side channels were frequently reworked in the braid plain but the cumulative length of channels appeared to be stable within the historical time period. This dynamic nature implies that the aquatic ecosystems have evolved within a high disturbance regime.

Performance Improvement of a Return Channel in a Multistage Centrifugal Compressor Using Multiobjective Optimization.

PubMed

Nishida, Yoshifumi; Kobayashi, Hiromi; Nishida, Hideo; Sugimura, Kazuyuki

2013-05-01

The effect of the design parameters of a return channel on the performance of a multistage centrifugal compressor was numerically investigated, and the shape of the return channel was optimized using a multiobjective optimization method based on a genetic algorithm to improve the performance of the centrifugal compressor. The results of sensitivity analysis using Latin hypercube sampling suggested that the inlet-to-outlet area ratio of the return vane affected the total pressure loss in the return channel, and that the inlet-to-outlet radius ratio of the return vane affected the outlet flow angle from the return vane. Moreover, this analysis suggested that the number of return vanes affected both the loss and the flow angle at the outlet. As a result of optimization, the number of return vane was increased from 14 to 22 and the area ratio was decreased from 0.71 to 0.66. The radius ratio was also decreased from 2.1 to 2.0. Performance tests on a centrifugal compressor with two return channels (the original design and optimized design) were carried out using two-stage test apparatus. The measured flow distribution exhibited a swirl flow in the center region and a reversed swirl flow near the hub and shroud sides. The exit flow of the optimized design was more uniform than that of the original design. For the optimized design, the overall two-stage efficiency and pressure coefficient were increased by 0.7% and 1.5%, respectively. Moreover, the second-stage efficiency and pressure coefficient were respectively increased by 1.0% and 3.2%. It is considered that the increase in the second-stage efficiency was caused by the increased uniformity of the flow, and the rise in the pressure coefficient was caused by a decrease in the residual swirl flow. It was thus concluded from the numerical and experimental results that the optimized return channel improved the performance of the multistage centrifugal compressor.

Bottom morphology in the Song Hau distributary channel, Mekong River Delta, Vietnam

NASA Astrophysics Data System (ADS)

Allison, Mead A.; Dallon Weathers, H.; Meselhe, Ehab A.

2017-09-01

Field studies in the Song Hau distributary of the Mekong Delta in Vietnam conducted at high (Sept.-Oct 2014) and low (March 2015) Mekong River discharge are utilized to examine channel bottom morphology and links with sediment transport in the system. Multibeam bathymetric mapping surveys over the entire channel complex in the lower 80 km of the distributary channel, and over 12- to 24-h tidal periods at six transect locations in the reach are used to characterize bottom type and change on seasonal and tidal timescales, supplemented by bottom sampling. The results of this study indicate that the largest proportion of channel floor (up to 80% of the total area) is composed of substratum outcrops of relict sediment units deposited during the progradation of the delta in the last 3.5 ka. These take the form of outcrops that are either (1) steep-sided, tabular channel floor, (2) steep-sided sidewall, or (3) relatively flat channel floor. Flatter outcrops of channel floor substratum are identified by the presence of sedimentary furrows (<0.5 m deep) incised into the channel bottom that are exposed at high discharge and oriented along channel and laterally continuous for kilometers. These furrows are persistent in location and extent across tidal cycles and appear to be incised into relict units, sometimes with a thin surficial layer of modern sediment observable in bottom grabs. The extent of substratum exposure, greater than that observed previously in low tidal energy systems like the Mississippi River, may relate both to a relatively low sand supply from the catchment, and/or to an efficient transfer of both sand and mud through this tidally energetic channel. Sand bottom areas forming dunes, comprise about 19% of the channel floor over the study area and are generally less than a few meters thick except on bar extensions of mid-channel islands. Both sandy and substratum areas are mantled by soft muds 0.25-1 m thick during low discharge in the estuarine section of the study area. This mud mantling appears to be a key control on bottom sourcing of sand to suspension. An understanding of channel bottom morphology, particularly mobility and erodibility of sediments, is valuable for setting up morphodynamic models of channel evolution that can be used to test system response to anthropogenic alterations in the catchment and rising sea levels.

Mechanism of activation at the selectivity filter of the KcsA K+ channel

PubMed Central

Heer, Florian T; Posson, David J; Wojtas-Niziurski, Wojciech

2017-01-01

Potassium channels are opened by ligands and/or membrane potential. In voltage-gated K+ channels and the prokaryotic KcsA channel, conduction is believed to result from opening of an intracellular constriction that prevents ion entry into the pore. On the other hand, numerous ligand-gated K+ channels lack such gate, suggesting that they may be activated by a change within the selectivity filter, a narrow region at the extracellular side of the pore. Using molecular dynamics simulations and electrophysiology measurements, we show that ligand-induced conformational changes in the KcsA channel removes steric restraints at the selectivity filter, thus resulting in structural fluctuations, reduced K+ affinity, and increased ion permeation. Such activation of the selectivity filter may be a universal gating mechanism within K+ channels. The occlusion of the pore at the level of the intracellular gate appears to be secondary. PMID:28994652

Channel Training for Analog FDD Repeaters: Optimal Estimators and Cramér-Rao Bounds

NASA Astrophysics Data System (ADS)

Wesemann, Stefan; Marzetta, Thomas L.

2017-12-01

For frequency division duplex channels, a simple pilot loop-back procedure has been proposed that allows the estimation of the UL & DL channels at an antenna array without relying on any digital signal processing at the terminal side. For this scheme, we derive the maximum likelihood (ML) estimators for the UL & DL channel subspaces, formulate the corresponding Cram\\'er-Rao bounds and show the asymptotic efficiency of both (SVD-based) estimators by means of Monte Carlo simulations. In addition, we illustrate how to compute the underlying (rank-1) SVD with quadratic time complexity by employing the power iteration method. To enable power control for the data transmission, knowledge of the channel gains is needed. Assuming that the UL & DL channels have on average the same gain, we formulate the ML estimator for the channel norm, and illustrate its robustness against strong noise by means of simulations.

Microfluidic droplet sorting using integrated bilayer micro-valves

NASA Astrophysics Data System (ADS)

Chen, Yuncong; Tian, Yang; Xu, Zhen; Wang, Xinran; Yu, Sicong; Dong, Liang

2016-10-01

This paper reports on a microfluidic device capable of sorting microfluidic droplets utilizing conventional bilayer pneumatic micro-valves as sorting controllers. The device consists of two micro-valves placed symmetrically on two sides of a sorting area, each on top of a branching channel at an inclined angle with respect to the main channel. Changes in transmitted light intensity, induced by varying light absorbance by each droplet, are used to divert the droplet from the sorting area into one of the three outlet channels. When no valve is activated, the droplet flows into the outlet channel in the direction of the main channel. When one of the valves is triggered, the flexible membrane of valve will first be deflected. Once the droplet leaves the detection point, the deflected membrane will immediately return to its default flattened position, thereby exerting a drawing pressure on the droplet and deviating it from its original streamline to the outlet on the same side as the valve. This sorting method will be particularly suitable for numerous large-scale integrated microfluidic systems, where pneumatic micro-valves are already used. Only few structural modifications are needed to achieve droplet sorting capabilities in these systems. Due to the mechanical nature of diverting energy applied to droplets, the proposed sorting method may induce only minimal interference to biological species or microorganisms encapsulated inside the droplets that may accompany electrical, optical and magnetic-based techniques.

Methanol sensor operated in a passive mode

DOEpatents

Ren, Xiaoming; Gottesfeld, Shimshon

2002-01-01

A sensor outputs a signal related to a concentration of methanol in an aqueous solution adjacent the sensor. A membrane electrode assembly (MEA) is included with an anode side and a cathode side. An anode current collector supports the anode side of the MEA and has a flow channel therethrough for flowing a stream of the aqueous solution and forms a physical barrier to control access of the methanol to the anode side of the MEA. A cathode current collector supports the cathode side of the MEA and is configured for air access to the cathode side of the MEA. A current sensor is connected to measure the current in a short circuit across the sensor electrodes to provide an output signal functionally related to the concentration of methanol in the aqueous solution.

Quantifying habitat benefits of channel reconfigurations on a highly regulated river system, Lower Missouri River, USA

USGS Publications Warehouse

Erwin, Susannah O.; Jacobson, Robert B.; Elliott, Caroline M.

2017-01-01

We present a quantitative analysis of habitat availability in a highly regulated lowland river, comparing a restored reach with two reference reaches: an un-restored, channelized reach, and a least-altered reach. We evaluate the effects of channel modifications in terms of distributions of depth and velocity as well as distributions and availability of habitats thought to be supportive of an endangered fish, the pallid sturgeon (Scaphirhynchus albus). It has been hypothesized that hydraulic conditions that support food production and foraging may limit growth and survival of juvenile pallid sturgeon. To evaluate conditions that support these habitats, we constructed two-dimensional hydrodynamic models for the three study reaches, two located in the Lower Missouri River (channelized and restored reaches) and one in the Yellowstone River (least-altered reach). Comparability among the reaches was improved by scaling by bankfull discharge and bankfull channel area. The analysis shows that construction of side-channel chutes and increased floodplain connectivity increase the availability of foraging habitat, resulting in a system that is more similar to the reference reach on the Yellowstone River. The availability of food-producing habitat is low in all reaches at flows less than bankfull, but the two reaches in the Lower Missouri River – channelized and restored – display a threshold-like response as flows overtop channel banks, reflecting the persistent effects of channelization on hydraulics in the main channel. These high lateral gradients result in punctuated ecological events corresponding to flows in excess of bankfull discharge. This threshold effect in the restored reach remains distinct from that of the least-altered reference reach, where hydraulic changes are less abrupt and overbank flows more gradually inundate the adjacent floodplain. The habitat curves observed in the reference reach on the Yellowstone River may not be attainable within the channelized system on the Missouri River, but the documented hydraulic patterns can be used to inform ongoing channel modifications. Although scaling to bankfull dimensions and discharges provides a basis for comparing the three reaches, implementation of the reference reach concept was complicated by differences in flow-frequency distributions among sites. In particular, habitat availability in the least-altered Yellowstone River reach is affected by increased frequency of low-flow events (less than 0.5 times bankfull flow) and moderately high-flow events (greater than 1.5 times bankfull flow) compared to downstream reaches on the Lower Missouri River.

Generic framework for the secure Yuen 2000 quantum-encryption protocol employing the wire-tap channel approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mihaljevic, Miodrag J.

2007-05-15

It is shown that the security, against known-plaintext attacks, of the Yuen 2000 (Y00) quantum-encryption protocol can be considered via the wire-tap channel model assuming that the heterodyne measurement yields the sample for security evaluation. Employing the results reported on the wire-tap channel, a generic framework is proposed for developing secure Y00 instantiations. The proposed framework employs a dedicated encoding which together with inherent quantum noise at the attacker's side provides Y00 security.

Design of joint source/channel coders

NASA Technical Reports Server (NTRS)

1991-01-01

The need to transmit large amounts of data over a band limited channel has led to the development of various data compression schemes. Many of these schemes function by attempting to remove redundancy from the data stream. An unwanted side effect of this approach is to make the information transfer process more vulnerable to channel noise. Efforts at protecting against errors involve the reinsertion of redundancy and an increase in bandwidth requirements. The papers presented within this document attempt to deal with these problems from a number of different approaches.

Fast shoreline erosion induced by ship wakes in a coastal lagoon: Field evidence and remote sensing analysis.

PubMed

Zaggia, Luca; Lorenzetti, Giuliano; Manfé, Giorgia; Scarpa, Gian Marco; Molinaroli, Emanuela; Parnell, Kevin Ellis; Rapaglia, John Paul; Gionta, Maria; Soomere, Tarmo

2017-01-01

An investigation based on in-situ surveys combined with remote sensing and GIS analysis revealed fast shoreline retreat on the side of a major waterway, the Malamocco Marghera Channel, in the Lagoon of Venice, Italy. Monthly and long-term regression rates caused by ship wakes in a reclaimed industrial area were considered. The short-term analysis, based on field surveys carried out between April 2014 and January 2015, revealed that the speed of shoreline regression was insignificantly dependent on the distance from the navigation channel, but was not constant through time. Periods of high water levels due to tidal forcing or storm surges, more common in the winter season, are characterized by faster regression rates. The retreat is a discontinuous process in time and space depending on the morpho-stratigraphy and the vegetation cover of the artificial deposits. A GIS analysis performed with the available imagery shows an average retreat of 3-4 m/yr in the period between 1974 and 2015. Digitization of historical maps and bathymetric surveys made in April 2015 enabled the construction of two digital terrain models for both past and present situations. The two models have been used to calculate the total volume of sediment lost during the period 1968-2015 (1.19×106 m3). The results show that in the presence of heavy ship traffic, ship-channel interactions can dominate the morphodynamics of a waterway and its margins. The analysis enables a better understanding of how shallow-water systems react to the human activities in the post-industrial period. An adequate evaluation of the temporal and spatial variation of shoreline position is also crucial for the development of future scenarios and for the sustainable management port traffic worldwide.

Fast shoreline erosion induced by ship wakes in a coastal lagoon: Field evidence and remote sensing analysis

PubMed Central

Lorenzetti, Giuliano; Manfé, Giorgia; Scarpa, Gian Marco; Molinaroli, Emanuela; Parnell, Kevin Ellis; Rapaglia, John Paul; Gionta, Maria; Soomere, Tarmo

2017-01-01

An investigation based on in-situ surveys combined with remote sensing and GIS analysis revealed fast shoreline retreat on the side of a major waterway, the Malamocco Marghera Channel, in the Lagoon of Venice, Italy. Monthly and long-term regression rates caused by ship wakes in a reclaimed industrial area were considered. The short-term analysis, based on field surveys carried out between April 2014 and January 2015, revealed that the speed of shoreline regression was insignificantly dependent on the distance from the navigation channel, but was not constant through time. Periods of high water levels due to tidal forcing or storm surges, more common in the winter season, are characterized by faster regression rates. The retreat is a discontinuous process in time and space depending on the morpho-stratigraphy and the vegetation cover of the artificial deposits. A GIS analysis performed with the available imagery shows an average retreat of 3˗4 m/yr in the period between 1974 and 2015. Digitization of historical maps and bathymetric surveys made in April 2015 enabled the construction of two digital terrain models for both past and present situations. The two models have been used to calculate the total volume of sediment lost during the period 1968˗2015 (1.19×106 m3). The results show that in the presence of heavy ship traffic, ship-channel interactions can dominate the morphodynamics of a waterway and its margins. The analysis enables a better understanding of how shallow-water systems react to the human activities in the post-industrial period. An adequate evaluation of the temporal and spatial variation of shoreline position is also crucial for the development of future scenarios and for the sustainable management port traffic worldwide. PMID:29088244

A dual wavelength imaging system for plasma-surface interaction studies on the National Spherical Torus Experiment Upgrade

DOE PAGES

Scotti, F.; Soukhanovskii, V. A.

2015-12-09

A two-channel spectral imaging system based on a charge injection device radiation-hardened intensified camera was built for studies of plasma-surface interactions on divertor plasma facing components in the National Spherical Torus Experiment Upgrade (NSTX-U) tokamak. By means of commercially available mechanically referenced optical components, the two-wavelength setup images the light from the plasma, relayed by a fiber optic bundle, at two different wavelengths side-by-side on the same detector. Remotely controlled filter wheels are used for narrow band pass and neutral density filters on each optical path allowing for simultaneous imaging of emission at wavelengths differing in brightness up to 3more » orders of magnitude. Applications on NSTX-U will include the measurement of impurity influxes in the lower divertor strike point region and the imaging of plasma-material interaction on the head of the surface analysis probe MAPP (Material Analysis and Particle Probe). Furthermore, the diagnostic setup and initial results from its application on the lithium tokamak experiment are presented.« less

How to find the sedimentary archive of fluvial pollution in a bedrock-confined river reach

NASA Astrophysics Data System (ADS)

Elznicova, Jitka; Matys Grygar, Tomas; Kiss, Timea; Lelkova, Tereza; Balogh, Marton; Sikora, Martin

2016-04-01

The Ohre River springs in the Eastern Germany and it is a tributary of the Labe (Elbe) River in Northwest Bohemia. The river received pollution from several sources during the last five centuries. Most of the pollution sources located along the upper and middle reaches, where the depositional and erosional pattern of the river is highly variable. The upper part of the catchment consists of mainly felsic rocks and the river has a broad floodplain. The middle reach and its right-bank tributaries are deeply incised into the Doupovske Hory Mts., which consists of mafic volcanic rocks; whereas the left-bank tributaries are incised into intrusive and metamorphic rocks of the Krusne Hory Mts. (Ore mountains) with several local ore mines (Ag, Pb and U) in particular in around Olovi and Jachymov. Due to the geologic and geomorphologic complexity, deposition of historical sediments in the middle reach has been spatially limited and uneven, and anomalous background concentrations of risk elements are expected. As a consequence, in the middle reach of the Ohre River it is difficult to find a useful sedimentary archive of historical pollution, though it is desired for two main reasons: (1) to decipher the undocumented and poorly described pollution history from the Krusne Hory Mts. and (2) to better understand the retention of pollutants in the transport zones of a confined river system. Based on historical maps we identified a side-bar (35x320 m) in the middle reach of the river near Straz on Ohre and aimed to describe its formation, its recent erosion/deposition history and to evaluate its sedimentary archive value. In the first half of the 19th century it was an island separated from the valley edge by a side channel. Since then there has been no apparent lateral accretion of the bar (its shape has not been changed), but the upstream part of the side channel aggraded by a sediment plug. We evaluated the current bar topography and geomorphology by a detailed field survey, dated the sediments by dendrology and OSL dating, and performed in situ XRF analysis of sediment cores. The data show that the downstream head of the bar is the oldest and most of fine sediments (mostly sand, minor silt) of the bar material have been historically polluted by Pb mining. The sedimentary sequences, most valuable for reconstruction of recent pollution, were found in the side channel where the fill the representing the last ca 150 years pollution history (Hg and U). The body of the bar has been formed earlier. According to our hypothesis the bar originated as a direct consequence of historical mining in the nearby Jachymov Ore Region. The use of lateral fluvial deposits as a sedimentary archive definitely requires intensive application of fluvial geomorphology. Vice versa, pollution patterns will allow delineating areas, in particular the bar bank and inlet to the side channel, where intensive reworking (erosion/redeposition) occurred as documented by the microtopography and woody debris.

Preparation of arrays of long carbon nanotubes using catalyst structure

DOEpatents

Zhu, Yuntian T.; Arendt, Paul; Li, Qingwen; Zhang, Xiefie

2016-03-22

A structure for preparing an substantially aligned array of carbon nanotubes include a substrate having a first side and a second side, a buffer layer on the first side of the substrate, a catalyst on the buffer layer, and a plurality of channels through the structure for allowing a gaseous carbon source to enter the substrate at the second side and flow through the structure to the catalyst. After preparing the array, a fiber of carbon nanotubes may be spun from the array. Prior to spinning, the array can be immersed in a polymer solution. After spinning, the polymer can be cured.

Manipulation of cells' position across a microfluidic channel using a series of continuously varying herringbone structures

NASA Astrophysics Data System (ADS)

Jung, Yugyung; Hyun, Ji-chul; Choi, Jongchan; Atajanov, Arslan; Yang, Sung

2017-12-01

Controlling cells' movement is an important technique in biological analysis that is performed within a microfluidic system. Many external forces are utilized for manipulation of cells, including their position in the channel. These forces can effectively control cells in a desired manner. Most of techniques used to manipulate cells require sophisticated set-ups and equipment to generate desired effect. The exception to this is the use of hydrodynamic force. In this study, a series of continuously varying herringbone structures is proposed for positioning cells in a microfluidic channel using hydrodynamic force. This structure was experimentally developed by changing parameters, such as the length of the herringbone's apex, the length of the herringbone's base and the ratio of the height of the flat channel to the height of the herringbone structure. Results of this study, have demonstrated that the length of the herringbone's apex and the ratio of the heights of the flat channel and the herringbone structure were crucial parameters influencing positioning of cells at 100 μl/h flow rate. The final design was fixed at 170 and 80 μm for the length of herringbone's apex and the length of herringbone's base, respectively. The average position of cells in this device was 34 μm away from the side wall in a 200 μm wide channel. Finally, to substantiate a practical application of the herringbone structure for positioning, cells were randomly introduced into a microfluidic device, containing an array of trapping structures together with a series of herringbone structures along the channel. The cells were moved toward the trapping structure by the herringbone structure and the trapping efficiency was increased. Therefore, it is anticipated that this device will be utilized to continuously control cells' position without application of external forces.

Channel Gating Dependence on Pore Lining Helix Glycine Residues in Skeletal Muscle Ryanodine Receptor.

PubMed

Mei, Yingwu; Xu, Le; Mowrey, David D; Mendez Giraldez, Raul; Wang, Ying; Pasek, Daniel A; Dokholyan, Nikolay V; Meissner, Gerhard

2015-07-10

Type 1 ryanodine receptors (RyR1s) release Ca(2+) from the sarcoplasmic reticulum to initiate skeletal muscle contraction. The role of RyR1-G4934 and -G4941 in the pore-lining helix in channel gating and ion permeation was probed by replacing them with amino acid residues of increasing side chain volume. RyR1-G4934A, -G4941A, and -G4941V mutant channels exhibited a caffeine-induced Ca(2+) release response in HEK293 cells and bound the RyR-specific ligand [(3)H]ryanodine. In single channel recordings, significant differences in the number of channel events and mean open and close times were observed between WT and RyR1-G4934A and -G4941A. RyR1-G4934A had reduced K(+) conductance and ion selectivity compared with WT. Mutations further increasing the side chain volume at these positions (G4934V and G4941I) resulted in reduced caffeine-induced Ca(2+) release in HEK293 cells, low [(3)H]ryanodine binding levels, and channels that were not regulated by Ca(2+) and did not conduct Ca(2+) in single channel measurements. Computational predictions of the thermodynamic impact of mutations on protein stability indicated that although the G4934A mutation was tolerated, the G4934V mutation decreased protein stability by introducing clashes with neighboring amino acid residues. In similar fashion, the G4941A mutation did not introduce clashes, whereas the G4941I mutation resulted in intersubunit clashes among the mutated isoleucines. Co-expression of RyR1-WT with RyR1-G4934V or -G4941I partially restored the WT phenotype, which suggested lessening of amino acid clashes in heterotetrameric channel complexes. The results indicate that both glycines are important for RyR1 channel function by providing flexibility and minimizing amino acid clashes. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Natural cavity characteristics and cavity bird abundance on West Virginia forested islands of the Ohio River

Treesearch

James T. Anderson; Karen A. Riesz

2013-01-01

Wildlife habitats connected with forested islands and their back channels (areas where commercial traffic is prohibited) on the Ohio River are valuable to diverse species. However, quantitative data on the importance of these areas to cavity-nesting birds are lacking. We compared cavity-nesting bird use and habitat between back and navigational channel sides of islands...

Annotated Bibliography of Sediment Transport Occurring over Ebb-Tidal Deltas.

DTIC Science & Technology

1985-09-01

and trough cross stratification from the shallower channel, should be expected. Swash-generated, horizontal plane laminations or * slightly inclined... laminations from the shallow channel sides. Transitional inlets would produce a variety of sequences, the exact nature of which would reflect the relative...and tidal currents. The beach face is characterized by flatbeds and antidunes; the runnel contains cuspate megaripples and current ripples oriented

Northwestern Branch of Mangala Vallis

NASA Technical Reports Server (NTRS)

2002-01-01

(Released 12 June 2002) The Science One of the many branches of the Mangala Vallis channel system is seen in this image. The water that likely carved the channels emerged from a huge graben or fracture almost 1000 km to the south. The THEMIS image shows where one of the channels exits the cratered highlands terrain onto the lowland plains. A bright scarp marks the transition between the two terrain types and demonstrates that in this location the highlands terrain is being eroded back. Note how the floor of the main channel appears to be at the same level as the lowland terrain, suggestive of a base level where erosion is no longer effective. Most of the steep slope faces in the image display darker slope streaks that are thought to be dust avalanche scars and indicate that a relatively thick mantle of dust is present in this region. Wind-sculpted ridges known as yardangs cover many of the surfaces throughout the area as shown by images from the Mars Global Surveyor mission. Most of them are at the limit of resolution in the THEMIS image but some are evident on the floor of the main channel at the point at which a smaller side channel enters. In this location they appear to extend right up to the base of the channel wall, giving the appearance that they are emerging from underneath the thick pile of material into which the channel is eroded. This suggests a geologic history in which a preexisting landscape of eroded yardangs was covered over by a thick pile of younger material that is now eroding back down to the original level. Alternatively, it is possible that the yardangs formed more recently at the abrupt transition between the channel floor and wall. More analysis is necessary to sort out the story. The Story This channel system is named 'Mangala,' the word for Mars in Sanskrit, a language of the Hindus of India that goes back more than 4,000 years, with written literature almost as long. Great epic tales have been written in this language, and Odyssey is continuing in the spirit of those adventures with its daily discoveries. Long ago, many thousands of years before Sanskrit was spoken on the Earth, a rush of water emerged from a giant fracture in the Martian land, carving the channels seen above. Since this fracture is located almost 600 miles to the south of this picture, you can only image the force of the flood. Today, the only real movement is the tired fall of dust avalanches down the channel slopes, which leave long dark trickles down the side. It's a dry, dusty world now, with a thick layer of dust everywhere. This image was taken at a place of transformation on Mars, where the cratered highlands meet the smooth, lowland plains. You can see that especially well in the context image to the right. Erosion is working tirelessly over time to bring the highlands level with the lowland terrain, but that will take eons more time into the future. Erosion may be 'deadly' to geological features, but it doesn't always happen quickly. If you want to look at one thing close up in this image, click on the above image and check out the floor of the main channel, just at the point where a smaller side channel enters (about a third of the way up). What you'll find are wind-sculpted ridges known as yardangs (some of them are almost triangular). What's interesting about these ridges is that they seem to have eroded long ago, then were covered by a thick pile of younger material, which is now itself eroding back, uncovering them once again. Yardangs are pretty common in this region of Mars, but if you have trouble finding them in many THEMIS images, don't worry, you're not alone. That's because the THEMIS camera is designed to take pictures of a larger area than its sister camera on the Mars Global Surveyor spacecraft, so some smaller yardangs are barely detectable. The Mars Orbital Camera, however, takes more detailed pictures of a narrower slice of the Martian landscape, and has shown many yardangs in the area. The great thing is that the THEMIS and MOC cameras are very complementary to one another. It's important to get the larger context of the terrain, as well as the sharp details of a tinier area for the greatest understanding possible. For example, while the yardangs in this image seem to be emerging from a blanket of younger material, it's also possible that they formed more recently at the abrupt transition between the channel floor and the wall. More analysis - and more pictures from both cameras! - will be needed to sort out the story.

A novel alternating current multiple array electrothermal micropump for lab-on-a-chip applications.

PubMed

Salari, A; Navi, M; Dalton, C

2015-01-01

The AC electrothermal technique is very promising for biofluid micropumping, due to its ability to pump high conductivity fluids. However, compared to electroosmotic micropumps, a lack of high fluid flow is a disadvantage. In this paper, a novel AC multiple array electrothermal (MAET) micropump, utilizing multiple microelectrode arrays placed on the side-walls of the fluidic channel of the micropump, is introduced. Asymmetric coplanar microelectrodes are placed on all sides of the microfluidic channel, and are actuated in different phases: one, two opposing, two adjacent, three, or all sides at the same time. Micropumps with different combinations of side electrodes and cross sections are numerically investigated in this paper. The effect of the governing parameters with respect to thermal, fluidic, and electrical properties are studied and discussed. To verify the simulations, the AC MAET concept was then fabricated and experimentally tested. The resulted fluid flow achieved by the experiments showed good agreement with the corresponding simulations. The number of side electrode arrays and the actuation patterns were also found to greatly influence the micropump performance. This study shows that the new multiple array electrothermal micropump design can be used in a wide range of applications such as drug delivery and lab-on-a-chip, where high flow rate and high precision micropumping devices for high conductivity fluids are needed.

Gating transitions in the selectivity filter region of a sodium channel are coupled to the domain IV voltage sensor.

PubMed

Capes, Deborah L; Arcisio-Miranda, Manoel; Jarecki, Brian W; French, Robert J; Chanda, Baron

2012-02-14

Voltage-dependent ion channels are crucial for generation and propagation of electrical activity in biological systems. The primary mechanism for voltage transduction in these proteins involves the movement of a voltage-sensing domain (D), which opens a gate located on the cytoplasmic side. A distinct conformational change in the selectivity filter near the extracellular side has been implicated in slow inactivation gating, which is important for spike frequency adaptation in neural circuits. However, it remains an open question whether gating transitions in the selectivity filter region are also actuated by voltage sensors. Here, we examine conformational coupling between each of the four voltage sensors and the outer pore of a eukaryotic voltage-dependent sodium channel. The voltage sensors of these sodium channels are not structurally symmetric and exhibit functional specialization. To track the conformational rearrangements of individual voltage-sensing domains, we recorded domain-specific gating pore currents. Our data show that, of the four voltage sensors, only the domain IV voltage sensor is coupled to the conformation of the selectivity filter region of the sodium channel. Trapping the outer pore in a particular conformation with a high-affinity toxin or disulphide crossbridge impedes the return of this voltage sensor to its resting conformation. Our findings directly establish that, in addition to the canonical electromechanical coupling between voltage sensor and inner pore gates of a sodium channel, gating transitions in the selectivity filter region are also coupled to the movement of a voltage sensor. Furthermore, our results also imply that the voltage sensor of domain IV is unique in this linkage and in the ability to initiate slow inactivation in sodium channels.

Molecular dynamics studies of pathways of water movement in cyanobacterial photosystem II

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gabdulkhakov, A. G., E-mail: azat@vega.protres.ru; Kljashtorny, V. G.; Dontsova, M. V.

2015-01-15

Photosystem II (PSII) catalyzes the light-induced generation of oxygen from water. The oxygen-evolving complex is buried deep in the protein on the lumenal side of PSII, and water molecules need to pass through protein subunits to reach the active site—the manganese cluster. Previous studies on the elucidation of water channels in PSII were based on an analysis of the cavities in the static PSII structure determined by X-ray diffraction. In the present study, we perform molecular dynamics simulations of the water movement in the transport system of PSII.

Topics in quantum cryptography, quantum error correction, and channel simulation

NASA Astrophysics Data System (ADS)

Luo, Zhicheng

In this thesis, we mainly investigate four different topics: efficiently implementable codes for quantum key expansion [51], quantum error-correcting codes based on privacy amplification [48], private classical capacity of quantum channels [44], and classical channel simulation with quantum side information [49, 50]. For the first topic, we propose an efficiently implementable quantum key expansion protocol, capable of increasing the size of a pre-shared secret key by a constant factor. Previously, the Shor-Preskill proof [64] of the security of the Bennett-Brassard 1984 (BB84) [6] quantum key distribution protocol relied on the theoretical existence of good classical error-correcting codes with the "dual-containing" property. But the explicit and efficiently decodable construction of such codes is unknown. We show that we can lift the dual-containing constraint by employing the non-dual-containing codes with excellent performance and efficient decoding algorithms. For the second topic, we propose a construction of Calderbank-Shor-Steane (CSS) [19, 68] quantum error-correcting codes, which are originally based on pairs of mutually dual-containing classical codes, by combining a classical code with a two-universal hash function. We show, using the results of Renner and Koenig [57], that the communication rates of such codes approach the hashing bound on tensor powers of Pauli channels in the limit of large block-length. For the third topic, we prove a regularized formula for the secret key assisted capacity region of a quantum channel for transmitting private classical information. This result parallels the work of Devetak on entanglement assisted quantum communication capacity. This formula provides a new family protocol, the private father protocol, under the resource inequality framework that includes the private classical communication without the assisted secret keys as a child protocol. For the fourth topic, we study and solve the problem of classical channel simulation with quantum side information at the receiver. Our main theorem has two important corollaries: rate-distortion theory with quantum side information and common randomness distillation. Simple proofs of achievability of classical multi-terminal source coding problems can be made via a unified approach using the channel simulation theorem as building blocks. The fully quantum generalization of the problem is also conjectured with outer and inner bounds on the achievable rate pairs.

Mode-specific multi-channel dynamics of the F- + CHD2Cl reaction on a global ab initio potential energy surface

NASA Astrophysics Data System (ADS)

Szabó, István; Czakó, Gábor

2016-10-01

We report a detailed quasiclassical trajectory study for the dynamics of the ground-state and CH/CD stretching-excited F- + CHD2Cl(vCH/CD = 0, 1) → Cl- + CHD2F, HF + CD2Cl-, and DF + CHDCl- SN2, proton-, and deuteron-abstraction reactions using a full-dimensional global ab initio analytical potential energy surface. The simulations show that (a) CHD2Cl(vCH/CD = 1), especially for vCH = 1, maintains its mode-specific excited character prior to interaction, (b) the SN2 reaction is vibrationally mode-specific, (c) double inversion can occur and is enhanced upon CH/CD stretching excitations, (d) in the abstraction reactions the HF channel is preferred and the vCH/CD = 1 excitations significantly promote the HF/DF channels, (e) back-side rebound, back-side stripping, and front-side stripping are the dominant direct abstraction mechanisms based on correlated scattering- and attack-angle distributions, (f) the exact classical vibrational energy-based Gaussian binning (1GB) provides realistic mode-specific polyatomic product state distributions, (g) in the abstraction reactions CH and CD stretchings are not pure spectator modes and mainly ground-state products are produced, thus most of the initial energy transfers into product translation, and (h) the HF and DF product molecules are rotationally cold without any significant dependence on the reactant's and HF/DF vibrational states.

Inhibitory effect of DIDS, NPPB, and phloretin on intracellular chloride channels.

PubMed

Malekova, Lubica; Tomaskova, Jana; Novakova, Marie; Stefanik, Peter; Kopacek, Juraj; Lakatos, Boris; Pastorekova, Silvia; Krizanova, Olga; Breier, Albert; Ondrias, Karol

2007-11-01

We studied the effects of the chloride channel blockers, 5-nitro-2-(phenylpropylamino)-benzoate (NPPB), dihydro-4,4' diisothiocyanostilbene-2,2'-disulphonic acid (DIDS), and phloretin on H2O2-induced primary culture cardiomyocyte apoptosis and activity of intracellular chloride channels obtained from rat heart mitochondrial and lysosomal vesicles. The chloride channel blockers (100 micromol/l) inhibited the H2O2-induced cardiomyocytes apoptosis. We characterized the effect of the blockers on single channel properties of the chloride channels derived from the mitochondrial and lysosomal vesicles incorporated into a bilayer lipid membrane. The single chloride channel currents were measured in 250:50 mmol/l KCl cis/trans solutions. NPPB, DIDS, and phloretin inhibited the chloride channels by decreasing the channel open probability in a concentration-dependent manner with EC50 values of 42, 7, and 20 micromol/l, respectively. NPPB and phloretin inhibited the channel's conductance and open dwell time, indicating that they could affect the chloride selective filter, pore permeability, and gating mechanism of the chloride channels. DIDS and NPPB inhibited the channels from the other side than bongkrekic acid and carboxyatractyloside. The results may contribute to understand a possible involvement of intracellular chloride channels in apoptosis and cardioprotection.

33 CFR 80.525 - Cape Lookout, NC to Cape Fear, NC.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Shackleford Banks. (b) A line drawn from Morehead City Channel Range Front Light to the seaward extremity of... the southwest side of New River Inlet at latitude 34°31.5′ N. longitude 77°20.6′ W. to the seaward tangent of the shoreline on the northeast side on New River Inlet. (e) A line drawn across New Topsail...

Analysis of an all optical de-multiplexer architecture utilizing bevel design for spatially multiplexed optical fiber communication systems

NASA Astrophysics Data System (ADS)

Murshid, Syed H.; Finch, Michael F.; Lovell, Gregory L.

2014-09-01

Spatial domain multiplexing (SDM) is a system that allows multiple channels of light to traverse a single fiber, utilizing separate spatial regions inside the carrier fiber, thereby applying a new degree of photon freedom for optical fiber communications. These channels follow a helical pattern, the screen projection of which is viewable as concentric rings at the output end of the system. The MIMO nature of the SDM system implies that a typical pin-diode or APD will be unable to distinguish between these channels, as the diode will interpret the combination of the SDM signals from all channels as a single signal. As such, spatial de-multiplexing methods must be introduced to properly detect the SDM based MIMO signals. One such method utilizes a fiber consisting of multiple, concentric, hollow core fibers to route each channel independently and thereby de-mux the signals into separate fibers or detectors. These de-mux fibers consist of hollow core cylindrical structures with beveled edges on one side that gradually taper to route the circular, ring type, output energy patterns into a spot with the highest possible efficiency. This paper analyzes the beveled edge by varying its length and analyzing the total output power for each predetermined length allowing us to simulate ideal bevel length to minimize both system losses as well as total de-mux footprint. OptiBPM simulation engine is employed for these analyses.

Role of the S4-S5 linker in CNG channel activation.

PubMed

Kusch, Jana; Zimmer, Thomas; Holschuh, Jascha; Biskup, Christoph; Schulz, Eckhard; Nache, Vasilica; Benndorf, Klaus

2010-10-20

Cyclic nucleotide-gated (CNG) channels mediate sensory signal transduction in retinal and olfactory cells. The channels are activated by the binding of cyclic nucleotides to a cyclic nucleotide-binding domain (CNBD) in the C-terminus that is located at the intracellular side. The molecular events translating the ligand binding to the pore opening are still unknown. We investigated the role of the S4-S5 linker in the activation process by quantifying its interaction with other intracellular regions. To this end, we constructed chimeric channels in which the N-terminus, the S4-S5 linker, the C-linker, and the CNBD of the retinal CNGA1 subunit were systematically replaced by the respective regions of the olfactory CNGA2 subunit. Macroscopic concentration-response relations were analyzed, yielding the apparent affinity to cGMP and the Hill coefficient. The degree of functional coupling of intracellular regions in the activation gating was determined by thermodynamic double-mutant cycle analysis. We observed that all four intracellular regions, including the relatively short S4-S5 linker, are involved in controlling the apparent affinity of the channel to cGMP and, moreover, in determining the degree of cooperativity between the subunits, as derived from the Hill coefficient. The interaction energies reveal an interaction of the S4-S5 linker with both the N-terminus and the C-linker, but no interaction with the CNBD. Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

hERG K+ channel-associated cardiac effects of the antidepressant drug desipramine.

PubMed

Staudacher, Ingo; Wang, Lu; Wan, Xiaoping; Obers, Sabrina; Wenzel, Wolfgang; Tristram, Frank; Koschny, Ronald; Staudacher, Kathrin; Kisselbach, Jana; Koelsch, Patrick; Schweizer, Patrick A; Katus, Hugo A; Ficker, Eckhard; Thomas, Dierk

2011-02-01

Cardiac side effects of antidepressant drugs are well recognized. Adverse effects precipitated by the tricyclic drug desipramine include prolonged QT intervals, torsade de pointes tachycardia, heart failure, and sudden cardiac death. QT prolongation has been primarily attributed to acute blockade of hERG/I(Kr) currents. This study was designed to provide a more complete picture of cellular effects associated with desipramine. hERG channels were expressed in Xenopus laevis oocytes and human embryonic kidney (HEK 293) cells, and potassium currents were recorded using patch clamp and two-electrode voltage clamp electrophysiology. Ventricular action potentials were recorded from guinea pig cardiomyocytes. Protein trafficking and cell viability were evaluated in HEK 293 cells and in HL-1 mouse cardiomyocytes by immunocytochemistry, Western blot analysis, or colorimetric MTT assay, respectively. We found that desipramine reduced hERG currents by binding to a receptor site inside the channel pore. hERG protein surface expression was reduced after short-term treatment, revealing a previously unrecognized mechanism. When long-term effects were studied, forward trafficking was impaired and hERG currents were decreased. Action potential duration was prolonged upon acute and chronic desipramine exposure. Finally, desipramine triggered apoptosis in cells expressing hERG channels. Desipramine exerts at least four different cellular effects: (1) direct hERG channel block, (2) acute reduction of hERG surface expression, (3) chronic disruption of hERG trafficking, and (4) induction of apoptosis. These data highlight the complexity of hERG-associated drug effects.

Improved experimental and diagnostic techniques for dynamic tensile stress–strain measurement with a Kolsky tension bar

NASA Astrophysics Data System (ADS)

Qiu, Ying; Loeffler, Colin M.; Nie, Xu; Song, Bo

2018-07-01

Kolsky tension bar experiments were improved for dynamic tensile stress–strain measurements with higher fidelity and minimal uncertainties. The difficulties associated with specimen gripping, relatively short gage section, and geometric discontinuity at the bar ends all compromise the accuracy of the traditional strain measurement method in a Kolsky tension bar experiment. In this study, an improved three-channel splitting-beam laser extensometer technique was developed to directly and independently track the displacement of the incident and transmission bar interfaces. By adopting a dual-channel configuration on the incident bar side, the resolution and measurement range of this laser extensometer were coordinated between the two channels to provide highly precise measurement at both small and large strains under high strain-rate loading condition. On the transmission bar side an amplified channel, similar to that used on the incident bar side, was adopted to measure the transmission bar displacement with high resolution. With this novel design, a maximum resolution of approximately 500 nm can be obtained for the bar displacement measurement, which corresponds to a strain of 0.0079% for a specimen with 6.35 mm gage length. To further improve the accuracy, a pair of lock nuts were used to tighten the tensile specimen to the bars in an effort not only to prevent the specimen from potential pre-torsional deformation and damage during installation, but also to provide better thread engagement between the specimen and the bar ends. As a demonstration of this technique, dynamic tensile stress–strain response of a 304L stainless steel was characterized with high resolution in both elastic and plastic deformations.

[Lead compound optimization strategy(5) – reducing the hERG cardiac toxicity in drug development].

PubMed

Zhou, Sheng-bin; Wang, Jiang; Liu, Hong

2016-10-01

The potassium channel encoded by the human ether-a-go-go related gene(hERG) plays a very important role in the physiological and pathological processes in human. hERG potassium channel determines the outward currents which facilitate the repolarization of the myocardial cells. Some drugs were withdrawn from the market for the serious side effect of long QT interval and arrhythmia due to blockade of hERG channel. The strategies for lead compound optimization are to reduce inhibitory activity of hERG potassium channel and decrease cardiac toxicity. These methods include reduction of lipophilicity and basicity of amines, introduction of hydroxyl and acidic groups, and restricting conformation.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Karch, Andreas; Sato, Yoshiki

In this paper we discuss geodesic Witten diagrams in generic holographic conformal field theories with boundary or defect. Boundary CFTs allow two different de-compositions of two-point functions into conformal blocks: boundary channel and ambient channel. Building on earlier work, we derive a holographic dual of the boundary channel decomposition in terms of bulk-to-bulk propagators on lower dimensional AdS slices. In the situation in which we can treat the boundary or defect as a perturbation around pure AdS spacetime, we obtain the leading corrections to the two-point function both in boundary and ambient channel in terms of geodesic Witten diagrams whichmore » exactly reproduce the decomposition into corresponding conformal blocks on the field theory side.« less

Experimental and Numerical Investigation of Conjugate Heat Transfer in Rib-roughened Duct

DTIC Science & Technology

2011-10-01

investigations. The targeted application is the ribbed internal cooling channels used in turbine blades . A test section is built to model the underlying...physics of the conjugate heat transfer phenomena in a turbine blade . The investigation focuses on measurements conducive to turbulence characterization...approach simulates the heat transfer occurring from the free- stream hot gas side, through the blade thickness, towards the internal cooling channels and it

Low Herbicide Concentration Found in Streamflow After a Grass Cover is Killed

Treesearch

J.E. Douglass; D.R. Cochrane; G.W. Bailey; J.I. Teasley; D.W. Hill

1969-01-01

The grass cover on a steep Appalachian watershed was sprayed in 1966 with herbicides-first with atrazine and paraquat and later with atrazine and 2,4-D. Although grass growing in the stream channel was sprayed during the first treatment, atrazine and paraquat levels in water samples were low. For the second treatment, a 10-foot strip on either side of the channel was...

Constructions of secure entanglement channels assisted by quantum dots inside single-sided optical cavities

NASA Astrophysics Data System (ADS)

Heo, Jino; Kang, Min-Sung; Hong, Chang-Ho; Choi, Seong-Gon; Hong, Jong-Phil

2017-08-01

We propose quantum information processing schemes to generate and swap entangled states based on the interactions between flying photons and quantum dots (QDs) confined within optical cavities for quantum communication. To produce and distribute entangled states (Bell and Greenberger-Horne-Zeilinger [GHZ] states) between the photonic qubits of flying photons of consumers (Alice and Bob) and electron-spin qubits of a provider (trust center, or TC), the TC employs the interactions of the QD-cavity system, which is composed of a charged QD (negatively charged exciton) inside a single-sided cavity. Subsequently, the TC constructs an entanglement channel (Bell state and 4-qubit GHZ state) to link one consumer with another through entanglement swapping, which can be realized to exploit a probe photon with interactions of the QD-cavity systems and single-qubit measurements without Bell state measurement, for quantum communication between consumers. Consequently, the TC, which has quantum nodes (QD-cavity systems), can accomplish constructing the entanglement channel (authenticated channel) between two separated consumers from the distributions of entangled states and entanglement swapping. Furthermore, our schemes using QD-cavity systems, which are feasible with a certain probability of success and high fidelity, can be experimentally implemented with technology currently in use.

Error control techniques for satellite and space communications

NASA Technical Reports Server (NTRS)

Costello, D. J., Jr.

1986-01-01

High rate concatenated coding systems with trellis inner codes and Reed-Solomon (RS) outer codes for application in satellite communication systems are considered. Two types of inner codes are studied: high rate punctured binary convolutional codes which result in overall effective information rates between 1/2 and 1 bit per channel use; and bandwidth efficient signal space trellis codes which can achieve overall effective information rates greater than 1 bit per channel use. Channel capacity calculations with and without side information performed for the concatenated coding system. Concatenated coding schemes are investigated. In Scheme 1, the inner code is decoded with the Viterbi algorithm and the outer RS code performs error-correction only (decoding without side information). In scheme 2, the inner code is decoded with a modified Viterbi algorithm which produces reliability information along with the decoded output. In this algorithm, path metrics are used to estimate the entire information sequence, while branch metrics are used to provide the reliability information on the decoded sequence. This information is used to erase unreliable bits in the decoded output. An errors-and-erasures RS decoder is then used for the outer code. These two schemes are proposed for use on NASA satellite channels. Results indicate that high system reliability can be achieved with little or no bandwidth expansion.

Abnormal threshold voltage shift under hot carrier stress in Ti1-xNx/HfO2 p-channel metal-oxide-semiconductor field-effect transistors

NASA Astrophysics Data System (ADS)

Tsai, Jyun-Yu; Chang, Ting-Chang; Lo, Wen-Hung; Ho, Szu-Han; Chen, Ching-En; Chen, Hua-Mao; Tseng, Tseung-Yuen; Tai, Ya-Hsiang; Cheng, Osbert; Huang, Cheng-Tung

2013-09-01

This work investigates the channel hot carrier (CHC) effect in HfO2/Ti1-xNx p-channel metal oxide semiconductor field effect transistors (p-MOSFETs). Generally, the subthreshold swing (S.S.) should increase during CHC stress (CHCS), since interface states will be generated near the drain side under high electric field due to drain voltage (Vd). However, our experimental data indicate that S.S. has no evident change under CHCS, but threshold voltage (Vth) shifts positively. This result can be attributed to hot carrier injected into high-k dielectric near the drain side. Meanwhile, it is surprising that such Vth degradation is not observed in the saturation region during stress. Therefore, drain-induced-barrier-lowering (DIBL) as a result of CHC-induced electron trapping is proposed to explain the different Vth behaviors in the linear and saturation regions. Additionally, the influence of different nitrogen concentrations in HfO2/Ti1-xNx p-MOSFETs on CHCS is also investigated in this work. Since nitrogen diffuses to SiO2/Si interface induced pre-Nit occurring to degrades channel mobility during the annealing process, a device with more nitrogen shows slightly less impact ionization, leading to insignificant charge trapping-induced DIBL behavior.

Ultra-sensitive chemical and biological analysis via specialty fibers with built-in microstructured optofluidic channels.

PubMed

Zhang, Nan; Li, Kaiwei; Cui, Ying; Wu, Zhifang; Shum, Perry Ping; Auguste, Jean-Louis; Dinh, Xuan Quyen; Humbert, Georges; Wei, Lei

2018-02-13

All-in-fiber optofluidics is an analytical tool that provides enhanced sensing performance with simplified analyzing system design. Currently, its advance is limited either by complicated liquid manipulation and light injection configuration or by low sensitivity resulting from inadequate light-matter interaction. In this work, we design and fabricate a side-channel photonic crystal fiber (SC-PCF) and exploit its versatile sensing capabilities in in-line optofluidic configurations. The built-in microfluidic channel of the SC-PCF enables strong light-matter interaction and easy lateral access of liquid samples in these analytical systems. In addition, the sensing performance of the SC-PCF is demonstrated with methylene blue for absorptive molecular detection and with human cardiac troponin T protein by utilizing a Sagnac interferometry configuration for ultra-sensitive and specific biomolecular specimen detection. Owing to the features of great flexibility and compactness, high-sensitivity to the analyte variation, and efficient liquid manipulation/replacement, the demonstrated SC-PCF offers a generic solution to be adapted to various fiber-waveguide sensors to detect a wide range of analytes in real time, especially for applications from environmental monitoring to biological diagnosis.

Voltage gated sodium channels as drug discovery targets

PubMed Central

Bagal, Sharan K; Marron, Brian E; Owen, Robert M; Storer, R Ian; Swain, Nigel A

2015-01-01

Voltage-gated sodium (NaV) channels are a family of transmembrane ion channel proteins. They function by forming a gated, water-filled pore to help establish and control cell membrane potential via control of the flow of ions between the intracellular and the extracellular environments. Blockade of NaVs has been successfully accomplished in the clinic to enable control of pathological firing patterns that occur in a diverse range of conditions such as chronic pain, epilepsy, and cardiac arrhythmias. First generation sodium channel modulator drugs, despite low inherent subtype selectivity, preferentially act on over-excited cells which reduces undesirable side effects in the clinic. However, the limited therapeutic indices observed with the first generation demanded a new generation of sodium channel inhibitors. The structure, function and the state of the art in sodium channel modulator drug discovery are discussed in this chapter. PMID:26646477

Structure of the CLC-1 chloride channel from Homo sapiens.

PubMed

Park, Eunyong; MacKinnon, Roderick

2018-05-29

CLC channels mediate passive Cl - conduction, while CLC transporters mediate active Cl - transport coupled to H + transport in the opposite direction. The distinction between CLC-0/1/2 channels and CLC transporters seems undetectable by amino acid sequence. To understand why they are different functionally we determined the structure of the human CLC-1 channel. Its 'glutamate gate' residue, known to mediate proton transfer in CLC transporters, adopts a location in the structure that appears to preclude it from its transport function. Furthermore, smaller side chains produce a wider pore near the intracellular surface, potentially reducing a kinetic barrier for Cl - conduction. When the corresponding residues are mutated in a transporter, it is converted to a channel. Finally, Cl - at key sites in the pore appear to interact with reduced affinity compared to transporters. Thus, subtle differences in glutamate gate conformation, internal pore diameter and Cl - affinity distinguish CLC channels and transporters. © 2018, Park & MacKinnon.

Front-surface fabrication of moderate aspect ratio micro-channels in fused silica by single picosecond Gaussian-Bessel laser pulse

NASA Astrophysics Data System (ADS)

Liu, Xin; Sanner, Nicolas; Sentis, Marc; Stoian, Razvan; Zhao, Wei; Cheng, Guanghua; Utéza, Olivier

2018-02-01

Single-shot Gaussian-Bessel laser beams of 1 ps pulse duration and of 0.9 μm core size and 60 μm depth of focus are used for drilling micro-channels on front side of fused silica in ambient condition. Channels ablated at different pulse energies are fully characterized by AFM and post-processing polishing procedures. We identify experimental energy conditions (typically 1.5 µJ) suitable to fabricate non-tapered channels with mean diameter of 1.2 µm and length of 40 μm while maintaining an utmost quality of the front opening of the channels. In addition, by further applying accurate post-polishing procedure, channels with high surface quality and moderate aspect ratio down to a few units are accessible, which would find interest in the surface micro-structuring of materials, with perspective of further scalability to meta-material specifications.

Method and apparatus for a catalytic firebox reactor

DOEpatents

Smith, Lance L.; Etemad, Shahrokh; Ulkarim, Hasan; Castaldi, Marco J.; Pfefferle, William C.

2001-01-01

A catalytic firebox reactor employing an exothermic catalytic reaction channel and multiple cooling conduits for creating a partially reacted fuel/oxidant mixture. An oxidation catalyst is deposited on the walls forming the boundary between the multiple cooling conduits and the exothermic catalytic reaction channel, on the side of the walls facing the exothermic catalytic reaction channel. This configuration allows the oxidation catalyst to be backside cooled by any fluid passing through the cooling conduits. The heat of reaction is added to both the fluid in the exothermic catalytic reaction channel and the fluid passing through the cooling conduits. After discharge of the fluids from the exothermic catalytic reaction channel, the fluids mix to create a single combined flow. A further innovation in the reactor incorporates geometric changes in the exothermic catalytic reaction channel to provide streamwise variation of the velocity of the fluids in the reactor.

Dual-gate operation and carrier transport in SiGe p–n junction nanowires

DOE PAGES

Delker, Collin James; Yoo, Jink Young; Bussmann, Ezra; ...

2017-10-23

Here, we investigate carrier transport in silicon–germanium nanowires with an axial p–n junction doping profile by fabricating these wires into transistors that feature separate top gates over each doping segment. By independently biasing each gate, carrier concentrations in the n- and p-side of the wire can be modulated. For these devices, which were fabricated with nickel source–drain electrical contacts, holes are the dominant charge carrier, with more favorable hole injection occurring on the p-side contact. Channel current exhibits greater sensitivity to the n-side gate, and in the reverse biased source–drain configuration, current is limited by the nickel/n-side Schottky contact.

Dual-gate operation and carrier transport in SiGe p-n junction nanowires

NASA Astrophysics Data System (ADS)

Delker, C. J.; Yoo, J. Y.; Bussmann, E.; Swartzentruber, B. S.; Harris, C. T.

2017-11-01

We investigate carrier transport in silicon-germanium nanowires with an axial p-n junction doping profile by fabricating these wires into transistors that feature separate top gates over each doping segment. By independently biasing each gate, carrier concentrations in the n- and p-side of the wire can be modulated. For these devices, which were fabricated with nickel source-drain electrical contacts, holes are the dominant charge carrier, with more favorable hole injection occurring on the p-side contact. Channel current exhibits greater sensitivity to the n-side gate, and in the reverse biased source-drain configuration, current is limited by the nickel/n-side Schottky contact.

Dual-gate operation and carrier transport in SiGe p–n junction nanowires

DOE Office of Scientific and Technical Information (OSTI.GOV)

Delker, Collin James; Yoo, Jink Young; Bussmann, Ezra

Here, we investigate carrier transport in silicon–germanium nanowires with an axial p–n junction doping profile by fabricating these wires into transistors that feature separate top gates over each doping segment. By independently biasing each gate, carrier concentrations in the n- and p-side of the wire can be modulated. For these devices, which were fabricated with nickel source–drain electrical contacts, holes are the dominant charge carrier, with more favorable hole injection occurring on the p-side contact. Channel current exhibits greater sensitivity to the n-side gate, and in the reverse biased source–drain configuration, current is limited by the nickel/n-side Schottky contact.

Thermodynamic coupling between activation and inactivation gating in potassium channels revealed by free energy molecular dynamics simulations.

PubMed

Pan, Albert C; Cuello, Luis G; Perozo, Eduardo; Roux, Benoît

2011-12-01

The amount of ionic current flowing through K(+) channels is determined by the interplay between two separate time-dependent processes: activation and inactivation gating. Activation is concerned with the stimulus-dependent opening of the main intracellular gate, whereas inactivation is a spontaneous conformational transition of the selectivity filter toward a nonconductive state occurring on a variety of timescales. A recent analysis of multiple x-ray structures of open and partially open KcsA channels revealed the mechanism by which movements of the inner activation gate, formed by the inner helices from the four subunits of the pore domain, bias the conformational changes at the selectivity filter toward a nonconductive inactivated state. This analysis highlighted the important role of Phe103, a residue located along the inner helix, near the hinge position associated with the opening of the intracellular gate. In the present study, we use free energy perturbation molecular dynamics simulations (FEP/MD) to quantitatively elucidate the thermodynamic basis for the coupling between the intracellular gate and the selectivity filter. The results of the FEP/MD calculations are in good agreement with experiments, and further analysis of the repulsive, van der Waals dispersive, and electrostatic free energy contributions reveals that the energetic basis underlying the absence of inactivation in the F103A mutation in KcsA is the absence of the unfavorable steric interaction occurring with the large Ile100 side chain in a neighboring subunit when the intracellular gate is open and the selectivity filter is in a conductive conformation. Macroscopic current analysis shows that the I100A mutant indeed relieves inactivation in KcsA, but to a lesser extent than the F103A mutant.

Slow relaxation in weakly open rational polygons.

PubMed

Kokshenev, Valery B; Vicentini, Eduardo

2003-07-01

The interplay between the regular (piecewise-linear) and irregular (vertex-angle) boundary effects in nonintegrable rational polygonal billiards (of m equal sides) is discussed. Decay dynamics in polygons (of perimeter P(m) and small opening Delta) is analyzed through the late-time survival probability S(m) approximately equal t(-delta). Two distinct slow relaxation channels are established. The primary universal channel exhibits relaxation of regular sliding orbits, with delta=1. The secondary channel is given by delta>1 and becomes open when m>P(m)/Delta. It originates from vertex order-disorder dual effects and is due to relaxation of chaoticlike excitations.

National Program for Inspection of Non-Federal Dams. Winsor Dam (MA 00588), Quabbin Spillway (MA 00589), Goodnough Dike (MA 00590), Connecticut River Basin, Ware, Massachusetts. Phase I Inspection Report.

DTIC Science & Technology

1978-09-01

at the en- trance to the channel. Another rockfall occurs along the right side of the channel, about 280 ft. downstream from the channel entrance. The...new level survey on seven settlement observation points at Goodnough Dike, in particular to check the 1973 data at * 27 ,nn lnmnnnm u u ~ l I • lI gI ...Spillway weir masonry to maintain the structure in good condition. 3. Periodically remove brush, saplings and rockfalls from the I spiliway discharge

The luminal K+ channel of the thick ascending limb of Henle's loop.

PubMed

Bleich, M; Schlatter, E; Greger, R

1990-01-01

In vitro perfused rat thick ascending limbs of Henle's loop (TAL) were used (n = 260) to analyse the conductance properties of the luminal membrane applying the patch-clamp technique. Medullary (mTAL) and cortical (cTAL) tubule segments were dissected and perfused in vitro. The free end of the tubule was held and immobilized at one edge by a holding pipette kept under continuous suction. A micropositioner was used to insert a patch pipette into the lumen, and a gigaohm seal with the luminal membrane was achieved in 455 instances out of considerably more trials. In approximately 20% of all gigaohm seals recordings of single ionic channels were obtained. We have identified only one single type of K+ channel in these cell-attached and cell-excised recordings. In the cell-attached configuration with KCl or NaCl in the pipette, the channel had a conductance of 60 +/- 6 pS (n = 24) and 31 +/- 7 pS (n = 4) respectively. In cell-free patches with KCl either in the patch pipette or in the bath and with a Ringer-type solution (NaCl) on the opposite side the conductance was 72 +/- 4 pS (n = 37) at a clamp voltage of 0 mV. The permeability was 0.33 +/- 0.02 . 10(-12) cm3/s. The selectivity sequence of this channel was: K+ = Rb+ = NH4+ = Cs+ greater than Li+ much greater than Na+ = 0; the conductance sequence was K+ much greater than Li+ much greater than Rb+ = Cs+ = NH4+ = Na+ = 0. In excised patches Rb+, Cs+ and NH4+ when present in the bath at 145 mmol/l all inhibited K+ currents out of the pipette. The channel kinetics were described by one open (9.5 +/- 1.5 ms, n = 18) and by two closed (1.4 +/- 0.1 and 14 +/- 2 ms) time constants. The open probability of this channel was increased by depolarization. The channel open probability was reduced voltage dependently by Ba2+ (half maximal inhibition at 0 mV: 0.07 mmol/l) from the cytosolic side. Verapamil, diltiazem, quinine and quinidine inhibited at approximately 1 mumol/l -0.1 mmol/l from either side. Similarly, the amino cations lidocaine, tetraethylammonium and choline inhibited at 10-100 mmol/l. The channel was downregulated in its open probability by cytosolic Ca2+ activities greater than 10(-7) mol/l and by adenosine triphosphate greater than or equal to 10(-4) mol/l. The open probability was downregulated by decreasing cytosolic pH (2-fold by a decrease in pH by less than or equal to 0.2 units).(ABSTRACT TRUNCATED AT 400 WORDS)

Micro-Fluidic Chemical Reactor Systems: Development, Scale-Up and Demonstration

DTIC Science & Technology

2002-11-01

B) A ) Figure 1: Gas Phase Microreactor . ( A ) Photograph of device. (B) Top view schematic. (C) Side view across channel. ( D ) Side view along... Microreactor system showing controller, heater power, fluid mixing, and microreactor cards (as in Figure 14) in a PCI chassis... microreactor design used for gas-phase heterogeneous reactions is a microchannel device that can be integrated with a heat exchange layer for highly

Controlling the mode of operation of organic transistors through side-chain engineering.

PubMed

Giovannitti, Alexander; Sbircea, Dan-Tiberiu; Inal, Sahika; Nielsen, Christian B; Bandiello, Enrico; Hanifi, David A; Sessolo, Michele; Malliaras, George G; McCulloch, Iain; Rivnay, Jonathan

2016-10-25

Electrolyte-gated organic transistors offer low bias operation facilitated by direct contact of the transistor channel with an electrolyte. Their operation mode is generally defined by the dimensionality of charge transport, where a field-effect transistor allows for electrostatic charge accumulation at the electrolyte/semiconductor interface, whereas an organic electrochemical transistor (OECT) facilitates penetration of ions into the bulk of the channel, considered a slow process, leading to volumetric doping and electronic transport. Conducting polymer OECTs allow for fast switching and high currents through incorporation of excess, hygroscopic ionic phases, but operate in depletion mode. Here, we show that the use of glycolated side chains on a thiophene backbone can result in accumulation mode OECTs with high currents, transconductance, and sharp subthreshold switching, while maintaining fast switching speeds. Compared with alkylated analogs of the same backbone, the triethylene glycol side chains shift the mode of operation of aqueous electrolyte-gated transistors from interfacial to bulk doping/transport and show complete and reversible electrochromism and high volumetric capacitance at low operating biases. We propose that the glycol side chains facilitate hydration and ion penetration, without compromising electronic mobility, and suggest that this synthetic approach can be used to guide the design of organic mixed conductors.

Controlling the mode of operation of organic transistors through side-chain engineering

PubMed Central

Giovannitti, Alexander; Sbircea, Dan-Tiberiu; Inal, Sahika; Nielsen, Christian B.; Bandiello, Enrico; Hanifi, David A.; Sessolo, Michele; Malliaras, George G.; McCulloch, Iain; Rivnay, Jonathan

2016-01-01

Electrolyte-gated organic transistors offer low bias operation facilitated by direct contact of the transistor channel with an electrolyte. Their operation mode is generally defined by the dimensionality of charge transport, where a field-effect transistor allows for electrostatic charge accumulation at the electrolyte/semiconductor interface, whereas an organic electrochemical transistor (OECT) facilitates penetration of ions into the bulk of the channel, considered a slow process, leading to volumetric doping and electronic transport. Conducting polymer OECTs allow for fast switching and high currents through incorporation of excess, hygroscopic ionic phases, but operate in depletion mode. Here, we show that the use of glycolated side chains on a thiophene backbone can result in accumulation mode OECTs with high currents, transconductance, and sharp subthreshold switching, while maintaining fast switching speeds. Compared with alkylated analogs of the same backbone, the triethylene glycol side chains shift the mode of operation of aqueous electrolyte-gated transistors from interfacial to bulk doping/transport and show complete and reversible electrochromism and high volumetric capacitance at low operating biases. We propose that the glycol side chains facilitate hydration and ion penetration, without compromising electronic mobility, and suggest that this synthetic approach can be used to guide the design of organic mixed conductors. PMID:27790983

The Ins and Outs of Cellular Ca2+ Transport

PubMed Central

Spalding, Edgar P.; Harper, Jeffrey F.

2011-01-01

The cytoplasmic Ca2+ signals that participate in nearly all aspects of plant growth and development encode information as binary switches or information-rich signatures. They are the result of influx (thermodynamically passive) and efflux (thermodynamically active) activities mediated by membrane transport proteins. On the influx side, confirming the molecular identities of Ca2+-permeable channels is still a major research topic. Cyclic nucleotide-gated channels and glutamate receptor-like channels are candidates well supported by evidence. On the efflux side, CAX antiporters and P-type ATPase pumps are the principal molecular entities. Both of these active transporters load Ca2+ into specific compartments and have the potential to reduce the magnitude and duration of a Ca2+ transient. Recent studies indicate calmodulin-activated Ca2+ pumps in endomembrane systems can dampen the magnitude and duration of a Ca2+ transient that could otherwise grow into a Ca2+ cell-death signature. An important challenge following molecular characterization of the influx and efflux pathways is to understand how they are coordinately regulated to produce a Ca2+ switch or encode specific information into a Ca2+ signature. PMID:21865080

The Barium Site in a Potassium Channel by X-Ray Crystallography

PubMed Central

Jiang, Youxing; MacKinnon, Roderick

2000-01-01

X-ray diffraction data were collected from frozen crystals (100°K) of the KcsA K+ channel equilibrated with solutions containing barium chloride. Difference electron density maps (Fbarium − Fnative, 5.0 Å resolution) show that Ba2+ resides at a single location within the selectivity filter. The Ba2+ blocking site corresponds to the internal aspect (adjacent to the central cavity) of the “inner ion” position where an alkali metal cation is found in the absence of the blocking Ba2+ ion. The location of Ba2+ with respect to Rb+ ions in the pore is in good agreement with the findings on the functional interaction of Ba2+ with K+ (and Rb+) in Ca2+-activated K+ channels (Neyton, J., and C. Miller. 1988. J. Gen. Physiol. 92:549–567). Taken together, these structural and functional data imply that at physiological ion concentrations a third ion may interact with two ions in the selectivity filter, perhaps by entering from one side and displacing an ion on the opposite side. PMID:10694255

Depositional patterns and structural styles-Hackberry Salt Dome, Cameron Parish, Louisiana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Spencer, J.A.; Sharpe, C.L.; Gillham, T.H.

The West and East Hackberry fields of north-central Cameron Parish, Louisiana, are associated with a large southeast-plunging salt ridge. Episodes of salt movement influenced the depositional patterns and reservoir trap styles of the Oligocene- and Miocene-age sedimentary section. The Oligocene lower Hackberry channels were influenced by the salt structure, resulting in the Manchester-Holmwood channel system flanking the east and south sides of the salt dome and the Choupique channel system flanking the west side of the salt dome. The depositional patterns and structural bed dips of the younger Oligocene Camerina A to marginulina section demonstrate a major period of saltmore » movement and erosion. The resulting truncation of the Camerian A sandstones, sealed by overlying shales, provides the dominant trap style for the majority of the reservoirs. This same general period of salt movement influenced the orientation of the Oligocene Marginulina to Miogypsinoides expansion fault system to the east. The Sweet Lake salt dome, down through to this expansion system, probably represents a southeast extension of this ancestral salt ridge.« less

Study of heat transfer due to turbulent flow of nanofluids through rib-groove channel

NASA Astrophysics Data System (ADS)

Al-Shamani, A. N.; Sopian, K.; Abed, A. M.; Alghoul, M. A.; Ruslan, M. H.; Mat, S.

2015-09-01

Nanofluids for improve characteristics flow in a rib-groove channel are investigate. The continuity, momentum and energy equations were solved by FLUENT program. The bottom wall of channel is heated while the upper wall is symmetry, the left side velocity inlet, and the right side is outlet (pressure out). Four different rib-groove shapes are used. Four different types of nanoparticles, Al2O3, CuO, SiO2, and ZnO with different volumes fractions in the range of 1% to 4% and different nanoparticle diameter in the range of 25 nm to 70 nm, are dispersed in the base fluid water are used. In this paper, several parameters such as different Reynolds numbers in the range of 10000 < Re < 40000 are investigated. The numerical results indicate that the trapezoidal with increasing height in the flow direction rib- trapezoidal groove has the best heat transfer and high Nusselt number; the nanofluids with SiO2 have the best behavior. The Nusselt number increases as the volume fraction increases and it decreases as the nanoparticle diameter increases.

Experimental investigation of heat transfer coefficient of mini-channel PCHE (printed circuit heat exchanger)

NASA Astrophysics Data System (ADS)

Kwon, Dohoon; Jin, Lingxue; Jung, WooSeok; Jeong, Sangkwon

2018-06-01

Heat transfer coefficient of a mini-channel printed circuit heat exchanger (PCHE) with counter-flow configuration is investigated. The PCHE used in the experiments is two layered (10 channels per layer) and has the hydraulic diameter of 1.83 mm. Experiments are conducted under various cryogenic heat transfer conditions: single-phase, boiling and condensation heat transfer. Heat transfer coefficients of each experiments are presented and compared with established correlations. In the case of the single-phase experiment, empiricial correlation of modified Dittus-Boelter correlation was proposed, which predicts the experimental results with 5% error at Reynolds number range from 8500 to 17,000. In the case of the boiling experiment, film boiling phenomenon occurred dominantly due to large temperature difference between the hot side and the cold side fluids. Empirical correlation is proposed which predicts experimental results with 20% error at Reynolds number range from 2100 to 2500. In the case of the condensation experiment, empirical correlation of modified Akers correlation was proposed, which predicts experimental results with 10% error at Reynolds number range from 3100 to 6200.

Direct numerical simulation of curved turbulent channel flow

NASA Technical Reports Server (NTRS)

Moser, R. D.; Moin, P.

1984-01-01

Low Reynolds number, mildly curved, turbulent channel flow has been simulated numerically without subgrid scale models. A new spectral numerical method developed for this problem was used, and the computations were performed with 2 million degrees of freedom. A variety of statistical and structural information has been extracted from the computed flow fields. These include mean velocity, turbulence stresses, velocity skewness, and flatness factors, space time correlations and spectra, all the terms in the Reynolds stress balance equations, and contour and vector plots of instantaneous velocity fields. The effects of curvature on this flow were determined by comparing the concave and convex sides of the channel. The observed effects are consistent with experimental observations for mild curvature. The most significant difference in the turbulence statistics between the concave and convex sides was in the Reynolds shear stress. This was accompanied by significant differences in the terms of the Reynolds shear stress balance equations. In addition, it was found that stationary Taylor-Gortler vortices were present and that they had a significant effect on the flow by contributing to the mean Reynolds shear stress, and by affecting the underlying turbulence.

Enhancing analog performance and suppression of subthreshold swing using hetero-junctionless double gate TFETs

NASA Astrophysics Data System (ADS)

Chauhan, Sudakar Singh; Sharma, Neha

2017-12-01

This paper proposes hetero-junctionless double gate tunnel field effect transistor (HJLDG-TFETs) for suppression of subthreshold swing (SS) using an InAs compound semiconductor material. The proposed device with high dielectric material, gives an excellent performance when InAs uses at source side. Because of low band gap of 0.36 eV , it reduces the potential barrier height of source channel interface causing higher band to band tunneling. Whereas, Si at the drain side with higher band gap of 1.12 eV , increasing the barrier height of drain channel interface causing lower quantum tunneling. As a result, the proposed device with high-k (HfO2) at 30 nm channel section provides a tremendous characteristics with high ION /IOFF ratio of 2 ×1011 , a point SS of 43.30 mV / decade and moderate SS of 56.75 mV / decade . All the above results show that the proposed device is assured for a low power switching application. The variation in gate supply voltage also analyzed for transconductance property of the device.

Modulation of the olfactory CNG channel by Ptdlns(3,4,5)P3.

PubMed

Zhainazarov, A B; Spehr, M; Wetzel, C H; Hatt, H; Ache, B W

2004-09-01

Recent data suggest that the 3-phosphoinositides can modulate cyclic nucleotide signaling in rat olfactory receptor neurons (ORNs). Given the ability of diverse lipids to modulate ion channels, we asked whether phosphatidylinositol 3,4,5-trisphosphate (PIP3) can regulate the olfactory cyclic nucleotide-gated (CNG) channel as a possible mechanism for this modulation. We show that applying PIP3 to the intracellular side of inside-out patches from rat ORNs inhibits activation of the olfactory CNG channel by cAMP. The effect of PIP3 is immediate and partially reversible, and reflects an increase in the EC50 of cAMP, not a reduction in the single-channel current amplitude. The effect of PIP3 is significantly stronger than that of PIP2; other phospholipids tested have no appreciable effect on channel activity. PIP3 similarly inhibits the recombinant heteromeric (A2/A4) and homomeric (A2) olfactory CNG channel expressed in HEK293 cells, suggesting that PIP3 acts directly on the channel. These findings indicate that 3-phosphoinositides can be functionally important regulators of CNG channels.

Analysis of linear electrode array EMG for assessment of hemiparetic biceps brachii muscles.

PubMed

Yao, Bo; Zhang, Xu; Li, Sheng; Li, Xiaoyan; Chen, Xiang; Klein, Cliff S; Zhou, Ping

2015-01-01

This study presents a frequency analysis of surface electromyogram (EMG) signals acquired by a linear electrode array from the biceps brachii muscles bilaterally in 14 hemiparetic stroke subjects. For different levels of isometric contraction ranging from 10 to 80% of the maximum voluntary contraction (MVC), the power spectra of 19 bipolar surface EMG channels arranged proximally to distally along the muscle fibers were examined in both paretic and contralateral muscles. It was found that across all stroke subjects, the median frequency (MF) and the mean power frequency (MPF), averaged from different surface EMG channels, were significantly smaller in the paretic muscle compared to the contralateral muscle at each of the matched percent MVC contractions. The muscle fiber conduction velocity (MFCV) was significantly slower in the paretic muscle than in the contralateral muscle. No significant correlation between the averaged MF, MPF, or MFCV vs. torque was found in both paretic and contralateral muscles. However, there was a significant positive correlation between the global MFCV and MF. Examination of individual EMG channels showed that electrodes closest to the estimated muscle innervation zones produced surface EMG signals with significantly higher MF and MPF than more proximal or distal locations in both paretic and contralateral sides. These findings suggest complex central and peripheral neuromuscular alterations (such as selective loss of large motor units, disordered control of motor units, increased motor unit synchronization, and atrophy of muscle fibers, etc.) which can collectively influence the surface EMG signals. The frequency difference with regard to the innervation zone also confirms the relevance of electrode position in surface EMG analysis.

Water channel in the binding site of a high affinity anti-methotrexate antibody.

PubMed

Gayda, Susan; Longenecker, Kenton L; Manoj, Sharmila; Judge, Russell A; Saldana, Sylvia C; Ruan, Qiaoqiao; Swift, Kerry M; Tetin, Sergey Y

2014-06-17

In the present study, we report the structure of the free and drug-bound Fab fragment of a high affinity anti-methotrexate antibody and perform a thermodynamic analysis of the binding process. The anti-methotrexate Fab fragment features a remarkably rigid tunnel-like binding site that extends into a water channel serving as a specialized route to move solvent out and into the site upon ligand binding and dissociation. This new finding in antibody structure-function relationships directly relates to the fast association (1 × 10⁷ M⁻¹ s⁻¹) and slow dissociation (4 × 10⁻⁵ s⁻¹) rates determined for mAb ADD056, resulting in a very strong binding with a K(D) ~ 3.6 pM at 20 °C. As follows from the X-ray data analysis, the methotrexate-antibody complex is stabilized by an extended network of hydrogen bonds and stacking interactions. The analysis also shows structural involvement of the CDR H3 in formation of the water channel revealing another important role of this hypervariable region. This suggests a new direction in natural affinity maturation and opens a new possibility in antibody engineering. Methotrexate is a widely used therapeutic agent for many malignant diseases and inflammatory disorders. Unfortunately, it may also interfere with central aspects of metabolism and thereby cause inevitable side effects. Therefore, methotrexate therapy requires careful monitoring of drug blood levels, which is traditionally done by immunoassays. An understanding of the structure-function properties of antibodies selected for drug monitoring substantiates the performance and robustness of such tests.

Heat Transfer Enhancement in High Performance Heat Sink Channels by Autonomous, Aero-Elastic Reed Fluttering

NASA Astrophysics Data System (ADS)

Jha, Sourabh; Crittenden, Thomas; Glezer, Ari

2016-11-01

Heat transport within high aspect ratio, rectangular mm-scale channels that model segments of a high-performance, air-cooled heat sink is enhanced by the formation of unsteady small-scale vortical motions induced by autonomous, aeroelastic fluttering of cantilevered planar thin-film reeds. The flow mechanisms and scaling of the interactions between the reed and the channel flow are explored to overcome the limits of forced convection heat transport from air-side heat exchangers. High-resolution PIV measurements in a testbed model show that undulations of the reed's surface lead to formation and advection of vorticity concentrations, and to alternate shedding of spanwise CW and CCW vortices. These vortices scale with the reed motion amplitude, and ultimately result in motions of decreasing scales and enhanced dissipation that are reminiscent of a turbulent flow. The vorticity shedding lead to strong enhancement in heat transfer that increases with the Reynolds number of the base flow (e.g., the channel's thermal coefficient of performance is enhanced by 2.4-fold and 9-fold for base flow Re = 4,000 and 17,400, respectively, with corresponding decreases of 50 and 77% in the required channel flow rates). This is demonstrated in heat sinks for improving the thermal performance of low-Re thermoelectric power plant air-cooled condensers, where the global air-side pressure losses can be significantly reduced by lowering the required air volume flow rate at a given heat flux and surface temperature. AFOSR and NSF-EPRI.

Four-wall turbine airfoil with thermal strain control for reduced cycle fatigue

DOEpatents

Cambell, Christian X

2013-09-17

A turbine airfoil (20B) with a thermal expansion control mechanism that increases the airfoil camber (60, 61) under operational heating. The airfoil has four-wall geometry, including pressure side outer and inner walls (26, 28B), and suction side outer and inner walls (32, 34B). It has near-wall cooling channels (31F, 31A, 33F, 33A) between the outer and inner walls. A cooling fluid flow pattern (50C, 50W, 50H) in the airfoil causes the pressure side inner wall (28B) to increase in curvature under operational heating. The pressure side inner wall (28B) is thicker than walls (26, 34B) that oppose it in camber deformation, so it dominates them in collaboration with the suction side outer wall (32), and the airfoil camber increases. This reduces and relocates a maximum stress area (47) from the suction side outer wall (32) to the suction side inner wall (34B, 72) and the pressure side outer wall (26).

Estimation of hydraulic jump characteristics of channels with sudden diverging side walls via SVM.

PubMed

Roushangar, Kiyoumars; Valizadeh, Reyhaneh; Ghasempour, Roghayeh

2017-10-01

Sudden diverging channels are one of the energy dissipaters which can dissipate most of the kinetic energy of the flow through a hydraulic jump. An accurate prediction of hydraulic jump characteristics is an important step in designing hydraulic structures. This paper focuses on the capability of the support vector machine (SVM) as a meta-model approach for predicting hydraulic jump characteristics in different sudden diverging stilling basins (i.e. basins with and without appurtenances). In this regard, different models were developed and tested using 1,018 experimental data. The obtained results proved the capability of the SVM technique in predicting hydraulic jump characteristics and it was found that the developed models for a channel with a central block performed more successfully than models for channels without appurtenances or with a negative step. The superior performance for the length of hydraulic jump was obtained for the model with parameters F 1 (Froude number) and (h 2- h 1 )/h 1 (h 1 and h 2 are sequent depth of upstream and downstream respectively). Concerning the relative energy dissipation and sequent depth ratio, the model with parameters F 1 and h 1 /B (B is expansion ratio) led to the best results. According to the outcome of sensitivity analysis, Froude number had the most significant effect on the modeling. Also comparison between SVM and empirical equations indicated the great performance of the SVM.

High Intracellular Chloride Slows the Decay of Glycinergic Currents

PubMed Central

Pitt, Samantha J.; Sivilotti, Lucia G.; Beato, Marco

2009-01-01

The time course of currents mediated by native and recombinant glycine receptors was examined with a combination of rapid agonist applications to outside-out patches and single-channel recording. The deactivation time constant of currents evoked by brief, saturating pulses of glycine is profoundly affected by the chloride concentration on the intracellular side of the cell membrane. Deactivation was threefold slower when intracellular chloride was increased from a low level (10 mm), similar to that observed in living mature neurons, to 131 mm (“symmetrical” chloride, often used in pipette internal solutions). Single-channel analysis revealed that high chloride has its greatest effect on the channel closing rate, slowing it by a factor of 2 compared with the value we estimated in the cell-attached mode (in which the channels are at physiological intracellular chloride concentrations). The same effect of chloride was observed when glycinergic evoked synaptic currents were recorded from juvenile rat spinal motoneurons in vitro, because the decay time constant was reduced from ∼7ms to ∼3 ms when cells were dialyzed with 10 mm chloride intracellular recording solution. Our results indicate that the time course of glycinergic synaptic inhibition in intact neurons is much faster than is estimated by measurements in symmetrical chloride and can be modulated by changes in intracellular chloride concentration in the range that can occur in physiological or pathological conditions. PMID:18987182

Ameliorating Effects of Sulfonylurea Drugs on Insulin Resistance in Otsuka Long-Evans Tokushima Fatty Rats

PubMed Central

Park, Jeong-Kwon; Kim, Sang-Pyo

2008-01-01

OLETF (Otsuka Long-Evans Tokushima Fatty) rats are characterized by obesity-related insulin resistance, which is a phenotype of type 2 diabetes. Sulfonylurea drugs or benzoic acid derivatives as inhibitors of the ATP-sensitive potassium (KATP) channel are commercially available to treat diabetes. The present study compared sulfonylurea drugs (glimepiride and gliclazide) with one of benzoic acid derivatives (repaglinide) in regard to their long-term effect on ameliorating insulin sensitivity in OLETF rats. Each drug was dissolved and fed with drinking water from 29 weeks of age. On high glucose loading at 45 weeks of age, response of blood glucose recovery was the greatest in the group treated with glimepiride. On immunohistochemistry analysis for the Kir6.2 subunit of KATP channels, insulin receptor β-subunits, and glucose transporters (GLUT) type 2 and 4 in liver, fat and skeletal muscle tissues, the sulfonylurea drugs (glimepiride and gliclazide) were more effective than repaglinide in recovery from their decreased expressions in OLETF rats. From these results, it seems to be plausible that KATP-channel inhibitors containing sulfonylurea moiety may be much more effective in reducing insulin resistance than those with benzoic acid moiety. In contrast to gliclazide, non-tissue selectivity of glimepiride on KATP channel inhibition may further strengthen an amelioration of insulin sensitivity unless considering other side effects. PMID:20157388

Ameliorating effects of sulfonylurea drugs on insulin resistance in Otsuka long-evans Tokushima Fatty rats.

PubMed

Park, Jeong-Kwon; Kim, Sang-Pyo; Song, Dae-Kyu

2008-02-01

OLETF (Otsuka Long-Evans Tokushima Fatty) rats are characterized by obesity-related insulin resistance, which is a phenotype of type 2 diabetes. Sulfonylurea drugs or benzoic acid derivatives as inhibitors of the ATP-sensitive potassium (K(ATP)) channel are commercially available to treat diabetes. The present study compared sulfonylurea drugs (glimepiride and gliclazide) with one of benzoic acid derivatives (repaglinide) in regard to their long-term effect on ameliorating insulin sensitivity in OLETF rats. Each drug was dissolved and fed with drinking water from 29 weeks of age. On high glucose loading at 45 weeks of age, response of blood glucose recovery was the greatest in the group treated with glimepiride. On immunohistochemistry analysis for the Kir6.2 subunit of K(ATP) channels, insulin receptor beta-subunits, and glucose transporters (GLUT) type 2 and 4 in liver, fat and skeletal muscle tissues, the sulfonylurea drugs (glimepiride and gliclazide) were more effective than repaglinide in recovery from their decreased expressions in OLETF rats. From these results, it seems to be plausible that K(ATP)-channel inhibitors containing sulfonylurea moiety may be much more effective in reducing insulin resistance than those with benzoic acid moiety. In contrast to gliclazide, non-tissue selectivity of glimepiride on K(ATP) channel inhibition may further strengthen an amelioration of insulin sensitivity unless considering other side effects.

Cl- channels of the gastric parietal cell that are active at low pH.

PubMed

Cuppoletti, J; Baker, A M; Malinowska, D H

1993-06-01

HCl secretion across mammalian gastric parietal cell apical membrane may involve Cl- channels. H(+)-K(+)-ATPase-containing membranes isolated from gastric mucosa of histamine-stimulated rabbits were fused to planar lipid bilayers. Channels were recorded with symmetric 800 mM CsCl solutions, pH 7.4. A linear current-voltage (I-V) relationship was obtained, and conductance was 28 +/- 1 pS at 800 mM CsCl. Conductance was 6.9 +/- 2 pS at 150 mM CsCl. Reversal potential was +22 mV with a fivefold cis-trans CsCl concentration gradient, indicating that the channel was anion selective with a discrimination ratio of 6:1 for Cl- over Cs+. Anion selectivity of the channel was I- > Cl- > or = Br- > NO3-, and gluconate was impermeant. Channels obtained at pH 7.4 persisted when pH of medium bathing the trans side of the bilayer (pHtrans) was reduced to pH 3, without a change in conductance, linearity of I-V relationship, or ion selectivity. In contrast, asymmetric reduction of pH of medium bathing the cis side of the bilayer from 7.4 to 3 always resulted in loss of channel activity. At pH 7.4, open probability (Po) of the channel was voltage dependent, i.e., predominantly open at +80 mV but mainly closed at -80 mV. In contrast, with low pHtrans, channel Po at -80 mV was increased 3.5-fold. The Cl- channel was Ca2+ indifferent. In absence of ionophores, ion selectivity for support of H(+)-K(+)-ATPase activity and H+ transport was consistent with that exhibited by the channel and could be limited by substitution with NO3-, whereas maximal H(+)-K(+)-ATPase activity was indifferent to anion present, demonstrating that anion transport can be rate limiting. Cl- channels with similar characteristics (conductance, linear I-V relationship, and ion selectivity) were also present in H(+)-K(+)-ATPase-containing vesicles isolated from resting (cimetidine-treated) gastric mucosa, exhibiting at -80 mV a pH-independent approximately 3.5-fold lower Po than stimulated vesicle channels. At -80 mV, reduction of pHtrans increased Po of both resting and stimulated Cl- channels by five- to sixfold. Changing membrane potential from 0 to -80 mV across stimulated vesicles increased Cl- channel activity an additional 10-fold.(ABSTRACT TRUNCATED AT 400 WORDS)

Feasibility of continuous-variable quantum key distribution with noisy coherent states

DOE Office of Scientific and Technical Information (OSTI.GOV)

Usenko, Vladyslav C.; Department of Optics, Palacky University, CZ-772 07 Olomouc; Filip, Radim

2010-02-15

We address security of the quantum key distribution scheme based on the noisy modulation of coherent states and investigate how it is robust against noise in the modulation regardless of the particular technical implementation. As the trusted preparation noise is shown to be security breaking even for purely lossy channels, we reveal the essential difference between two types of trusted noise, namely sender-side preparation noise and receiver-side detection noise, the latter being security preserving. We consider the method of sender-side state purification to compensate the preparation noise and show its applicability in the realistic conditions of channel loss, untrusted channelmore » excess noise, and trusted detection noise. We show that purification makes the scheme robust to the preparation noise (i.e., even the arbitrary noisy coherent states can in principle be used for the purpose of quantum key distribution). We also take into account the effect of realistic reconciliation and show that the purification method is still efficient in this case up to a limited value of preparation noise.« less

Progressive transmission of images over fading channels using rate-compatible LDPC codes.

PubMed

Pan, Xiang; Banihashemi, Amir H; Cuhadar, Aysegul

2006-12-01

In this paper, we propose a combined source/channel coding scheme for transmission of images over fading channels. The proposed scheme employs rate-compatible low-density parity-check codes along with embedded image coders such as JPEG2000 and set partitioning in hierarchical trees (SPIHT). The assignment of channel coding rates to source packets is performed by a fast trellis-based algorithm. We examine the performance of the proposed scheme over correlated and uncorrelated Rayleigh flat-fading channels with and without side information. Simulation results for the expected peak signal-to-noise ratio of reconstructed images, which are within 1 dB of the capacity upper bound over a wide range of channel signal-to-noise ratios, show considerable improvement compared to existing results under similar conditions. We also study the sensitivity of the proposed scheme in the presence of channel estimation error at the transmitter and demonstrate that under most conditions our scheme is more robust compared to existing schemes.

Structure of a eukaryotic cyclic nucleotide-gated channel

PubMed Central

Li, Minghui; Zhou, Xiaoyuan; Wang, Shu; Michailidis, Ioannis; Gong, Ye; Su, Deyuan; Li, Huan; Li, Xueming; Yang, Jian

2018-01-01

Summary Cyclic nucleotide-gated (CNG) channels are essential for vision and olfaction. They belong to the voltage-gated ion channel superfamily but their activities are controlled by intracellular cyclic nucleotides instead of transmembrane voltage. Here we report a 3.5 Å-resolution single-particle electron cryomicroscopy structure of a CNG channel from C. elegans in the cGMP-bound open state. The channel has an unusual voltage-sensor-like domain (VSLD), accounting for its deficient voltage dependence. A C-terminal linker connecting S6 and the cyclic nucleotide-binding domain interacts directly with both the VSLD and pore domain, forming a gating ring that couples conformational changes triggered by cyclic nucleotide binding to the gate. The selectivity filter is lined by the carboxylate side chains of a functionally important glutamate and three rings of backbone carbonyls. This structure provides a new framework for understanding mechanisms of ion permeation, gating and channelopathy of CNG channels and cyclic nucleotide modulation of related channels. PMID:28099415

A bursting potassium channel in isolated cholinergic synaptosomes of Torpedo electric organ.

PubMed Central

Edry-Schiller, J; Ginsburg, S; Rahamimoff, R

1991-01-01

1. Pinched-off cholinergic nerve terminals (synaptosomes) prepared from the electric organ of Torpedo ocelata were fused into large structures (greater than 20 microns) using dimethyl sulphoxide and polyethylene glycol 1500, as previously described for synaptic vesicles from the same organ. 2. The giant fused synaptosomes were easily amenable to the patch clamp technique and 293 seals with a resistance greater than 4 G omega were obtained in the 'cell-attached' configuration. In a large fraction of the experiments, an 'inside-out' patch configuration was achieved. 3. Several types of unitary ionic currents were observed. This study describes the most frequently observed single-channel activity which was found in 247 out of the 293 membrane patches (84.3%). 4. The single-channel current-voltage relation was linear between -60 and 20 mV and showed a slope conductance of 23.8 +/- 1.3 pS when the pipette contained 350-390 mM-Na+ and the bath facing the inside of the synaptosomal membrane contained 390 mM-K+. 5. From extrapolated reversal potential measurements, it was concluded that this channel has a large selectivity for K+ over Na+ (70.4 +/- 11.5, mean +/- S.E.M.). Chloride ions are not transported significantly through this potassium channel. 6. This potassium channel has a low probability of opening. The probability of being in the open state increases upon depolarization and reaches about 1% when the inside of the patch is 20 mV positive compared to the pipette side. 7. The mean channel open time increases with depolarization; thus the product current x time (= charge) also increases upon depolarization, showing properties of an outward rectifier. 8. The potassium channel in the giant synaptosome membrane has a bursting behaviour. Open-time distribution, closed-time distribution and a Poisson analysis indicate that the minimal kinetic scheme requires one open state and three closed states. PMID:1654418

Impact of gate engineering in enhancement mode n++GaN/InAlN/AlN/GaN HEMTs

NASA Astrophysics Data System (ADS)

Adak, Sarosij; Swain, Sanjit Kumar; Rahaman, Hafizur; Sarkar, Chandan Kumar

2016-12-01

This paper illustrate the effect of gate material engineering on the performance of enhancement mode n++GaN/InAlN/AlN/GaN high electron mobility transistors (HEMTs). A comparative analysis of key device parameters is discussed for the Triple Material Gate (TMG), Dual Material Gate (DMG) and the Single Material Gate (SMG) structure HEMTs by considering the same device dimensions. The simulation results shows that an significant improvement is noticed in the key analysis parameters such as drain current (Id), transconductance (gm), cut off frequency (fT), RF current gain, maximum cut off frequency (fmax) and RF power gain of the gate material engineered devices with respect to SMG normally off n++GaN/InAlN/AlN/GaN HEMTs. This improvement is due to the existence of the perceivable step in the surface potential along the channel which successfully screens the drain potential variation in the source side of the channel for the gate engineering devices. The analysis suggested that the proposed TMG and DMG engineered structure enhancement mode n++GaN/InAlN/AlN/GaN HEMTs can be considered as a potential device for future high speed, microwave and digital application.

Hydraulic droplet coarsening in open-channel capillaries

NASA Astrophysics Data System (ADS)

Warren, Patrick B.

2016-11-01

Over a range of liquid-solid contact angles, an open-channel capillary with curved or angled sides can show a maximum in the Laplace pressure as a function of the filling state. Examples include double-angle wedges, grooves scored into flat surfaces, steps on surfaces, and the groove between touching parallel cylinders. The liquid in such a channel exhibits a beading instability if the channel is filled beyond the Laplace pressure maximum. The subsequent droplet coarsening takes place by hydraulic transport through the connecting liquid columns that remain in the groove. A mean-field scaling argument predicts the characteristic droplet radius R ˜t1 /7 , as a function of time t . This is confirmed by one-dimensional simulations of the coarsening kinetics. Some remarks are also made on the spreading kinetics of an isolated drop deposited in such a channel.

Inward-rectifying potassium (Kir) channels regulate pacemaker activity in spinal nociceptive circuits during early life

PubMed Central

Li, Jie; Blankenship, Meredith L.; Baccei, Mark L.

2013-01-01

Pacemaker neurons in neonatal spinal nociceptive circuits generate intrinsic burst-firing and are distinguished by a lower “leak” membrane conductance compared to adjacent, non-bursting neurons. However, little is known about which subtypes of leak channels regulate the level of pacemaker activity within the developing rat superficial dorsal horn (SDH). Here we demonstrate that a hallmark feature of lamina I pacemaker neurons is a reduced conductance through inward-rectifying potassium (Kir) channels at physiological membrane potentials. Differences in the strength of inward rectification between pacemakers and non-pacemakers indicate the presence of functionally distinct Kir currents in these two populations at room temperature. However, Kir currents in both groups showed high sensitivity to block by extracellular Ba2+ (IC50 ~ 10 µM), which suggests the presence of ‘classical’ Kir (Kir2.x) channels in the neonatal SDH. The reduced Kir conductance within pacemakers is unlikely to be explained by an absence of particular Kir2.x isoforms, as immunohistochemical analysis revealed the expression of Kir2.1, Kir2.2 and Kir2.3 within spontaneously bursting neurons. Importantly, Ba2+ application unmasked rhythmic burst-firing in ~42% of non-bursting lamina I neurons, suggesting that pacemaker activity is a latent property of a sizeable population of SDH cells during early life. In addition, the prevalence of spontaneous burst-firing within lamina I was enhanced in the presence of high internal concentrations of free Mg2+, consistent with its documented ability to block Kir channels from the intracellular side. Collectively, the results indicate that Kir channels are key modulators of pacemaker activity in newborn central pain networks. PMID:23426663

Proof-of-principle test of coherent-state continuous variable quantum key distribution through turbulent atmosphere (Conference Presentation)

NASA Astrophysics Data System (ADS)

Derkach, Ivan D.; Peuntinger, Christian; Ruppert, László; Heim, Bettina; Gunthner, Kevin; Usenko, Vladyslav C.; Elser, Dominique; Marquardt, Christoph; Filip, Radim; Leuchs, Gerd

2016-10-01

Continuous-variable quantum key distribution is a practical application of quantum information theory that is aimed at generation of secret cryptographic key between two remote trusted parties and that uses multi-photon quantum states as carriers of key bits. Remote parties share the secret key via a quantum channel, that presumably is under control of of an eavesdropper, and which properties must be taken into account in the security analysis. Well-studied fiber-optical quantum channels commonly possess stable transmittance and low noise levels, while free-space channels represent a simpler, less demanding and more flexible alternative, but suffer from atmospheric effects such as turbulence that in particular causes a non-uniform transmittance distribution referred to as fading. Nonetheless free-space channels, providing an unobstructed line-of-sight, are more apt for short, mid-range and potentially long-range (using satellites) communication and will play an important role in the future development and implementation of QKD networks. It was previously theoretically shown that coherent-state CV QKD should be in principle possible to implement over a free-space fading channel, but strong transmittance fluctuations result in the significant modulation-dependent channel excess noise. In this regime the post-selection of highly transmitting sub-channels may be needed, which can even restore the security of the protocol in the strongly turbulent channels. We now report the first proof-of-principle experimental test of coherent state CV QKD protocol using different levels Gaussian modulation over a mid-range (1.6-kilometer long) free-space atmospheric quantum channel. The transmittance of the link was characterized using intensity measurements for the reference but channel estimation using the modulated coherent states was also studied. We consider security against Gaussian collective attacks, that were shown to be optimal against CV QKD protocols . We assumed a general entangling cloner collective attack (modeled using data obtained from the state measurement results on both trusted sides of the protocol), that allows to purify the noise added in the quantum channel . Our security analysis of coherent-state protocol also took into account the effect of imperfect channel estimation, limited post-processing efficiency and finite data ensemble size on the performance of the protocol. In this regime we observe the positive key rate even without the need of applying post-selection. We show the positive improvement of the key rate with increase of the modulation variance, still remaining low enough to tolerate the transmittance fluctuations. The obtained results show that coherent-state CV QKD protocol that uses real free-space atmospheric channel can withstand negative influence of transmittance fluctuations, limited post-processing efficiency, imperfect channel estimation and other finite-size effects, and be successfully implemented. Our result paves the way to the full-scale implementation of the CV QKD in real free-space channels at mid-range distances.

Boundary holographic Witten diagrams

DOE PAGES

Karch, Andreas; Sato, Yoshiki

2017-09-25

In this paper we discuss geodesic Witten diagrams in generic holographic conformal field theories with boundary or defect. Boundary CFTs allow two different de-compositions of two-point functions into conformal blocks: boundary channel and ambient channel. Building on earlier work, we derive a holographic dual of the boundary channel decomposition in terms of bulk-to-bulk propagators on lower dimensional AdS slices. In the situation in which we can treat the boundary or defect as a perturbation around pure AdS spacetime, we obtain the leading corrections to the two-point function both in boundary and ambient channel in terms of geodesic Witten diagrams whichmore » exactly reproduce the decomposition into corresponding conformal blocks on the field theory side.« less

Calcium channel modulation as a target in chronic pain control

PubMed Central

Montagut‐Bordas, Carlota; Dickenson, Anthony H

2017-01-01

Neuropathic pain remains poorly treated for large numbers of patients, and little progress has been made in developing novel classes of analgesics. To redress this issue, ziconotide (Prialt™) was developed and approved as a first‐in‐class synthetic version of ω‐conotoxin MVIIA, a peptide blocker of Cav2.2 channels. Unfortunately, the impracticalities of intrathecal delivery, low therapeutic index and severe neurological side effects associated with ziconotide have restricted its use to exceptional circumstances. Ziconotide exhibits no state or use‐dependent block of Cav2.2 channels; activation state‐dependent blockers were hypothesized to circumvent the side effects of state‐independent blockers by selectively targeting high‐frequency firing of nociceptive neurones in chronic pain states, thus alleviating aberrant pain but not affecting normal sensory transduction. Unfortunately, numerous drugs, including state‐dependent calcium channel blockers, have displayed efficacy in preclinical models but have subsequently been disappointing in clinical trials. In recent years, it has become more widely acknowledged that trans‐aetiological sensory profiles exist amongst chronic pain patients and may indicate similar underlying mechanisms and drug sensitivities. Heterogeneity amongst patients, a reliance on stimulus‐evoked endpoints in preclinical studies and a failure to utilize translatable endpoints, all are likely to have contributed to negative clinical trial results. We provide an overview of how electrophysiological and operant‐based assays provide insight into sensory and affective aspects of pain in animal models and how these may relate to chronic pain patients in order to improve the bench‐to‐bedside translation of calcium channel modulators. Linked Articles This article is part of a themed section on Recent Advances in Targeting Ion Channels to Treat Chronic Pain. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.12/issuetoc PMID:28320042

Dual Imprinted Polymer Thin Films via Pattern Directed Self-Organization.

PubMed

Grolman, Danielle; Bandyopadhyay, Diya; Al-Enizi, Abdullah; Elzatahry, Ahmed; Karim, Alamgir

2017-06-21

Synthetic topographically patterned films and coatings are typically contoured on one side, yet many of nature's surfaces have distinct textures on different surfaces of the same object. Common examples are the top and bottom sides of the butterfly wing or lotus leaf, onion shells, and the inside versus outside of the stem of a flower. Inspired by nature, we create dual (top and bottom) channel patterned polymer films. To this end, we first develop a novel fabrication method to create ceramic line channel relief structures by converting the oligomeric residue of stamped poly(dimethylsiloxane) (PDMS) nanopatterns on silicon substrates to glass (SiOx, silica) by ultraviolet-ozone (UVO) exposure. These silica patterned substrates are flow coated with polystyrene (PS) films and confined within an identically patterned top confining soft PDMS elastomer film. Annealing of the sandwich structures drives the PS to rapidly mold fill the top PDMS pattern in conjunction with a dewetting tendency of the PS on the silica pattern. Varying the film thickness h, from less than to greater than the pattern height, and varying the relative angle between the top-down and bottom-up patterned confinement surfaces create interesting uniform and nonuniform digitized defects in PS channel patterns, as also a defect-free channel regime. Our dual patterned polymer channels provide a novel fabrication route to topographically imprinted Moiré patterns (whose applications range from security encrypting holograms to sensitive strain gauges), and their basic laser light diffractions properties are illustrated and compared to graphical simulations and 2D-FFT of real-space AFM channel patterns. While traditional "geometrical" and "fringe" Moiré patterns function by superposition of two misaligned optical patterned transmittance gratings, our topographic pattern gratings are quite distinct and may allow for more unique holographic optical characteristics with further development.

Alternative actions on the K stack problem

DOE Office of Scientific and Technical Information (OSTI.GOV)

Spencer, H.G.

1958-09-29

On June 6, 1958, KW Operations had HCR Channel No. 16 borescoped to determine why this rod could not be inserted on May 3. Observations revealed 3X balls in the channel and horizontal separation between graphite blocks ranging from 1/2 inch to 2 1/4 inch. The separations were noted only in the first fifteen feet in from the outer skin and in the last five feet of the channel. As a result of these findings and past operational difficulties with certain HCR`s at both KE and KW Reactors, a program of measurements is in progress to determine the extent andmore » causes of the stack displacements. From measurements and observations so far obtained, the following conclusions may be drawn about separations at locations of consequence to the loss of 3X balls from channels: Side to side horizontal separations totaling two to three inches have been observed at both reactors. The significant separations lie outside of the VSR pattern and in the lower half of the stack. The VSR pattern does not include the six outer ball 3X channels. There are probably a few small separations (< 1/2 inch) at the outer VSR`s. There are probably no separations large enough to admit balls on inner VSR channels, i.e. VSR`s inside the outer front to rear rows of VSR`s. These conclusions can not be considered fully reliable, but no information obtained to date refutes them. A team was established on 9-17-58 to evaluate and provide information on alternate courses of action for elimination or alleviation of the problem. This report contains the information and recommendations developed by the team.« less

Structural implications of hERG K+ channel block by a high-affinity minimally structured blocker

PubMed Central

Helliwell, Matthew V.; Zhang, Yihong; El Harchi, Aziza; Du, Chunyun; Hancox, Jules C.; Dempsey, Christopher E.

2018-01-01

Cardiac potassium channels encoded by human ether-à-go-go–related gene (hERG) are major targets for structurally diverse drugs associated with acquired long QT syndrome. This study characterized hERG channel inhibition by a minimally structured high-affinity hERG inhibitor, Cavalli-2, composed of three phenyl groups linked by polymethylene spacers around a central amino group, chosen to probe the spatial arrangement of side chain groups in the high-affinity drug-binding site of the hERG pore. hERG current (IhERG) recorded at physiological temperature from HEK293 cells was inhibited with an IC50 of 35.6 nm with time and voltage dependence characteristic of blockade contingent upon channel gating. Potency of Cavalli-2 action was markedly reduced for attenuated inactivation mutants located near (S620T; 54-fold) and remote from (N588K; 15-fold) the channel pore. The S6 Y652A and F656A mutations decreased inhibitory potency 17- and 75-fold, respectively, whereas T623A and S624A at the base of the selectivity filter also decreased potency (16- and 7-fold, respectively). The S5 helix F557L mutation decreased potency 10-fold, and both F557L and Y652A mutations eliminated voltage dependence of inhibition. Computational docking using the recent cryo-EM structure of an open channel hERG construct could only partially recapitulate experimental data, and the high dependence of Cavalli-2 block on Phe-656 is not readily explainable in that structure. A small clockwise rotation of the inner (S6) helix of the hERG pore from its configuration in the cryo-EM structure may be required to optimize Phe-656 side chain orientations compatible with high-affinity block. PMID:29545312

Proton Transport

NASA Technical Reports Server (NTRS)

Pohorille, Andrew; DeVincenzi, Donald L. (Technical Monitor)

2001-01-01

The transport of protons across membranes is an essential process for both bioenergetics of modern cells and the origins of cellular life. All living systems make use of proton gradients across cell walls to convert environmental energy into a high-energy chemical compound, adenosine triphosphate (ATP), synthesized from adenosine diphosphate. ATP, in turn, is used as a source of energy to drive many cellular reactions. The ubiquity of this process in biology suggests that even the earliest cellular systems were relying on proton gradient for harvesting environmental energy needed to support their survival and growth. In contemporary cells, proton transfer is assisted by large, complex proteins embedded in membranes. The issue addressed in this Study was: how the same process can be accomplished with the aid of similar but much simpler molecules that could have existed in the protobiological milieu? The model system used in the study contained a bilayer membrane made of phospholipid, dimyristoylphosphatidylcholine (DMPC) which is a good model of the biological membranes forming cellular boundaries. Both sides of the bilayer were surrounded by water which simulated the environment inside and outside the cell. Embedded in the membrane was a fragment of the Influenza-A M$_2$ protein and enough sodium counterions to maintain system neutrality. This protein has been shown to exhibit remarkably high rates of proton transport and, therefore, is an excellent model to study the formation of proton gradients across membranes. The Influenza M$_2$ protein is 97 amino acids in length, but a fragment 25 amino acids long. which contains a transmembrane domain of 19 amino acids flanked by three amino acids on each side. is sufficient to transport protons. Four identical protein fragments, each folded into a helix, aggregate to form small channels spanning the membrane. Protons are conducted through a narrow pore in the middle of the channel in response to applied voltage. This channel is large enough to contain water molecules. and is normally filled with water. In analogy to the mechanism of proton transfer in some other channels, it has been postulated that protons are translocated along the network of water molecules filling the pore of the channel. This mechanism, however, must involve an additional important step because the channel contains four histidine amino acid residues, one from each of the helices, which are sufficiently large to occlude the pore and interrupt the water network. The histidine residues ensure channel selectivity by blocking transport of small ions, such as sodium or potassium. They have been also implicated in gating protons due to the ability of each histidine to become positively charged by accepting an additional proton. Two mechanisms of gating have been proposed. In one mechanism, all four histidines acquire an additional proton and, due to repulsion between their positive charges, move away from one another, thus opening the channel. The alternative mechanism relies of the ability of protons to move between different atoms in a molecule (tautomerization). Thus, a proton is captured on one side of the gate while another proton is released from the opposite side, and the molecule returns to the initial state through tautomerization. The simulations were designed to test these two mechanisms. Large-scale, atomic-level molecular dynamics simulations of the channel with the histidine residues in different protonation states revealed that all intermediate states of the system involved in the tautomerization mechanism are structurally stable and the arrangement of water molecules in the channel is conducive to the proton transport. In contrast, in the four-protonated state, postulated to exist in the gate-opening mechanism, the electrostatic repulsion between the histidine residues appears to be so large that the channel loses its structural integrity and one helix moves away from the remaining three. Additional information is contained within the original extended abstract.

Measurement-device-independent semiquantum key distribution

NASA Astrophysics Data System (ADS)

He, Jinjun; Li, Qin; Wu, Chunhui; Chan, Wai Hong; Zhang, Shengyu

Semiquantum key distribution (SQKD) allows two parties to share a common string when one of them is quantum and the other has rather limited quantum capability. Almost all existing SQKD protocols have been proved to be robust in theory, namely that if an eavesdropper tries to gain information, he will inevitably induce some detectable errors. However, ideal devices do not exist in reality and their imperfection may result in side-channel attacks, which can be used by an adversary to get some information on the secret key string. In this paper, we design a measurement-device-independent SQKD protocol for the first time, which can remove the threat of all detector side-channel attacks and show that it is also robust. In addition, we discuss the possible use of the proposed protocol in real-world applications and in QKD networks.

Dysregulation of renal aquaporins and epithelial sodium channel in lithium-induced nephrogenic diabetes insipidus.

PubMed

Nielsen, Jakob; Kwon, Tae-Hwan; Christensen, Birgitte Mønster; Frøkiaer, Jørgen; Nielsen, Søren

2008-05-01

Lithium is used commonly to treat bipolar mood disorders. In addition to its primary therapeutic effects in the central nervous system lithium has a number of side effects in the kidney. The side effects include nephrogenic diabetes insipidus with polyuria, mild sodium wasting, and changes in acid/base balance. These functional changes are associated with marked structural changes in collecting duct cell composition and morphology, likely contributing to the functional changes. Over the past few years, investigations of lithium-induced renal changes have provided novel insight into the molecular mechanisms that are responsible for the disturbances in water, sodium, and acid/base metabolism. This includes dysregulation of renal aquaporins, epithelial sodium channel, and acid/base transporters. This review focuses on these issues with the aim to present this in context with clinically relevant features.

Inertial focusing in a straight channel with asymmetrical expansion-contraction cavity arrays using two secondary flows

NASA Astrophysics Data System (ADS)

Zhang, J.; Li, M.; Li, W. H.; Alici, G.

2013-08-01

The focusing of particles has a variety of applications in industry and biomedicine, including wastewater purification, fermentation filtration, and pathogen detection in flow cytometry, etc. In this paper a novel inertial microfluidic device using two secondary flows to focus particles is presented. The geometry of the proposed microfluidic channel is a simple straight channel with asymmetrically patterned triangular expansion-contraction cavity arrays. Three different focusing patterns were observed under different flow conditions: (1) a single focusing streak on the cavity side; (2) double focusing streaks on both sides; (3) half of the particles were focused on the opposite side of the cavity, while the other particles were trapped by a horizontal vortex in the cavity. The focusing performance was studied comprehensively up to flow rates of 700 µl min-1. The focusing mechanism was investigated by analysing the balance of forces between the inertial lift forces and secondary flow drag in the cross section. The influence of particle size and cavity geometry on the focusing performance was also studied. The experimental results showed that more precise focusing could be obtained with large particles, some of which even showed a single-particle focusing streak in the horizontal plane. Meanwhile, the focusing patterns and their working conditions could be adjusted by the geometry of the cavity. This novel inertial microfluidic device could offer a continuous, sheathless, and high-throughput performance, which can be potentially applied to high-speed flow cytometry or the extraction of blood cells.

Hexadecameric structure of an invertebrate gap junction channel.

PubMed

Oshima, Atsunori; Matsuzawa, Tomohiro; Murata, Kazuyoshi; Tani, Kazutoshi; Fujiyoshi, Yoshinori

2016-03-27

Innexins are invertebrate-specific gap junction proteins with four transmembrane helices. These proteins oligomerize to constitute intercellular channels that allow for the passage of small signaling molecules associated with neural and muscular electrical activity. In contrast to the large number of structural and functional studies of connexin gap junction channels, few structural studies of recombinant innexin channels are reported. Here we show the three-dimensional structure of two-dimensionally crystallized Caenorhabditis elegans innexin-6 (INX-6) gap junction channels. The N-terminal deleted INX-6 proteins are crystallized in lipid bilayers. The three-dimensional reconstruction determined by cryo-electron crystallography reveals that a single INX-6 gap junction channel comprises 16 subunits, a hexadecamer, in contrast to chordate connexin channels, which comprise 12 subunits. The channel pore diameters at the cytoplasmic entrance and extracellular gap region are larger than those of connexin26. Two bulb densities are observed in each hemichannel, one in the pore and the other at the cytoplasmic side of the hemichannel in the channel pore pathway. These findings imply a structural diversity of gap junction channels among multicellular organisms. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Rate of bedrock channel incision by waterfall retreat and landscape response constrained by cosmogenic 3He, Kauai, Hawaii

NASA Astrophysics Data System (ADS)

Mackey, B. H.; Lamb, M. P.; Scheingross, J. S.; Farley, K. A.

2011-12-01

Channel incision and knickpoint retreat are the drivers of landscape evolution, yet we are still challenged to quantify the rate and processes by which rivers cut into rock. The Napali Coast on the northwestern side of Kauai, Hawaii, has multiple linear channels incising >200 m into the shield volcano surface. The channels have well-constrained initial conditions, including original topography, and relatively uniform layered basalt of known age (~4.5 Ma), which have attracted previous studies of channel evolution (e.g., Seidl et al., 1994, 1997). Many channels feature prominent waterfalls, although the mechanism of knickpoint initiation (submarine landslide vs cliff erosion) and subsequent retreat remain ambiguous. Motivated by these knowledge gaps and recent advances in cosmogenic helium geochronology, we revisited the Kaulaula Valley, a 9 km long narrow valley, beheaded on its upslope extent by the Waimea Canyon, and ending near the coast at the northern Mana Plain. Four kilometers up the canyon is a prominent 40 m high vertical knickpoint, dividing the valley into strongly contrasting geomorphic domains. The boulder-lined channel below the knickpoint is linear, steep (15%), and confined to a narrow valley with steep rocky cliffs (average slope 31°). Large, >2 m diameter angular boulders in the lower section of channel show evidence of mobility from debris flows. Above the knickpoint, average channel gradient is reduced (9%), bed load is much finer, and convex, soil-mantled hillslopes have a consistently lower mean slope of 18°. We constrained the exposure age of 18 features (in-channel boulders, stable boulders on terraces, and in-channel bedrock) along the length of the channel, by analysis of cosmogenic 3He in olivine phenocrysts. Cosmogenic exposure ages are oldest near the coast (80 ka) and systematically decrease with upstream distance towards the waterfall (< 5ka). In the upper section of channel, cosmogenic ages are approximately constant (10-20 ka). This data supports the conceptual model of knickpoint retreat and downstream terrace abandonment advocated by Seidl (1997), and we can constrain a maximum retreat rate of 40 mm/yr. Steady state erosion appears to dominate upstream of the waterfall. Catchment averaged erosion rates from detrital olivine (assuming catchment averaged production rate) give an erosion rate of 0.04 mm/yr .

Experimental investigation of fluvial dike breaching due to flow overtopping

NASA Astrophysics Data System (ADS)

El Kadi Abderrezzak, K.; Rifai, I.; Erpicum, S.; Archambeau, P.; Violeau, D.; Pirotton, M.; Dewals, B.

2017-12-01

The failure of fluvial dikes (levees) often leads to devastating floods that cause loss of life and damages to public infrastructure. Overtopping flows have been recognized as one of the most frequent cause of dike erosion and breaching. Fluvial dike breaching is different from frontal dike (embankments) breaching, because of specific geometry and boundary conditions. The current knowledge on the physical processes underpinning fluvial dike failure due to overtopping remains limited. In addition, there is a lack of a continuous monitoring of the 3D breach formation, limiting the analysis of the key mechanisms governing the breach development and the validation of conceptual or physically-based models. Laboratory tests on breach growth in homogeneous, non-cohesive sandy fluvial dikes due to flow overtopping have been performed. Two experimental setups have been constructed, permitting the investigation of various hydraulic and geometric parameters. Each experimental setup includes a main channel, separated from a floodplain by a dike. A rectangular initial notch is cut in the crest to initiate dike breaching. The breach development is monitored continuously using a specific developed laser profilometry technique. The observations have shown that the breach develops in two stages: first the breach deepens and widens with the breach centerline being gradually shifted toward the downstream side of the main channel. This behavior underlines the influence of the flow momentum component parallel to the dike crest. Second, the dike geometry upstream of the breach stops evolving and the breach widening continues only toward the downstream side of the main channel. The breach evolution has been found strongly affected by the flow conditions (i.e. inflow discharge in the main channel, downstream boundary condition) and floodplain confinement. The findings of this work shed light on key mechanisms of fluvial dike breaching, which differ substantially from those of dam breaching. These specific features need to be incorporated in flood risk analyses involving fluvial dike breach and failure. In addition, a well-documented, reliable data set, with a continuous high resolution monitoring of the 3D breach evolution under various flow conditions, has been gathered, which can be used for validating numerical models.

Use of high-resolution geophysical and geotechnical techniques for artificial reef site selection, west Cameron planning area, offshore Louisiana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pope, D.L.; Wagner, J.B.

1988-09-01

Before siting oil and gas platforms on the sea floor as artificial reefs offshore Louisiana, potentially hazardous and unstable geologic conditions must be identified and evaluated to assess their possible impacts on platform stability. Geologic and man-made features can be identified and assessed from high-resolution geophysical techniques (3.5-7.0 kHz echograms, single-channel seismic, and side-scan sonar). Such features include faults, diapirs, scarps, channels, gas seeps, irregular sea floor topography, mass wasting deposits (slumps, slides, and debris flows), pipelines, and other subsea marine equipment. Geotechnical techniques are utilized to determine lithologic and physical properties of the sediments for correlation with the geophysicalmore » data. These techniques are used to develop a series of geologic maps, cross sections, and pipeline and platform-location maps. Construction of echo-character maps from 3.5-kHz data provides an analysis of near-bottom sedimentation processes (turbidity currents and debris flows).« less

An analytic description of electrodynamic dispersion in free-flow zone electrophoresis.

PubMed

Dutta, Debashis

2015-07-24

The present work analyzes the electrodynamic dispersion of sample streams in a free-flow zone electrophoresis (FFZE) chamber resulting due to partial or complete blockage of electroosmotic flow (EOF) across the channel width by the sidewalls of the conduit. This blockage of EOF has been assumed to generate a pressure-driven backflow in the transverse direction for maintaining flow balance in the system. A parallel-plate based FFZE device with the analyte stream located far away from the channel side regions has been considered to simplify the current analysis. Applying a method-of-moments formulation, an analytic expression was derived for the variance of the sample zone at steady state as a function of its position in the separation chamber under these conditions. It has been shown that the increase in stream broadening due to the electrodynamic dispersion phenomenon is additive to the contributions from molecular diffusion and sample injection, and simply modifies the coefficient for the hydrodynamic dispersion term for a fixed lateral migration distance of the sample stream. Moreover, this dispersion mechanism can dominate the overall spatial variance of analyte zones when a significant fraction of the EOF is blocked by the channel sidewalls. The analysis also shows that analyte streams do not undergo any hydrodynamic broadening due to unwanted pressure-driven cross-flows in an FFZE chamber in the absence of a transverse electric field. The noted results have been validated using Monte Carlo simulations which further demonstrate that while the sample concentration profile at the channel outlet approaches a Gaussian distribution only in FFZE chambers substantially longer than the product of the axial pressure-driven velocity and the characteristic diffusion time in the system, the spatial variance of the exiting analyte stream is well described by the Taylor-Aris dispersion limit even in analysis ducts much shorter than this length scale. Copyright © 2015 Elsevier B.V. All rights reserved.

Exploring CP violation with Bc decays

NASA Astrophysics Data System (ADS)

Fleischer, Robert; Wyler, Daniel

2000-09-01

We point out that the pure ``tree'' decays B+/-c-->D+/-sD are particularly well suited to extract the Cabibbo-Kobayashi-Maskawa angle γ through amplitude relations. In contrast with conceptually similar strategies using B+/--->K+/-D or Bd-->K*0D decays, the advantage of the Bc approach is that the corresponding triangles have three sides of comparable length and do not involve small amplitudes. Decays of the type B+/-c-->D+/-D, the U-spin counterparts of B+/-c-->D+/-sD, can be added to the analysis, as well as channels, where the D+/-s and D+/- mesons are replaced by higher resonances.

Morphology, structure, composition and build-up processes of the active channel-mouth lobe complex of the Congo deep-sea fan with inputs from remotely operated underwater vehicle (ROV) multibeam and video surveys

NASA Astrophysics Data System (ADS)

Dennielou, Bernard; Droz, Laurence; Babonneau, Nathalie; Jacq, Céline; Bonnel, Cédric; Picot, Marie; Le Saout, Morgane; Saout, Yohan; Bez, Martine; Savoye, Bruno; Olu, Karine; Rabouille, Christophe

2017-08-01

The detailed structure and composition of turbiditic channel-mouth lobes is still largely unknown because they commonly lie at abyssal water depths, are very thin and are therefore beyond the resolution of hull-mound acoustic tools. The morphology, structure and composition of the Congo turbiditic channel-mouth lobe complex (90×40 km; 2525 km2) were investigated with hull-mounted swath bathymetry, air gun seismics, 3.5 kHz sub-bottom profiler, sediment piston cores and also with high-resolution multibeam bathymetry and video acquired with a Remote Operating Vehicle (ROV). The lobe complex lies 760 km off the Congo River mouth in the Angola abyssal plain between 4740 and 5030 m deep. It is active and is fed by turbidity currents that deposit several centimetres of sediment per century. The lobe complex is subdivided into five lobes that have prograded. The lobes are dominantly muddy. Sand represents ca. 13% of the deposits and is restricted to the feeding channel and distributaries. The overall lobe body is composed of thin muddy to silty turbidites. The whole lobe complex is characterized by in situ mass wasting (slumps, debrites). The 1-m-resolution bathymetry shows pervasive slidings and block avalanches on the edges of the feeding channel and the channel mouth indicating that sliding occurs early and continuously in the lobe build-up. Mass wasting is interpreted as a consequence of very-high accumulation rates, over-steepening and erosion along the channels and is therefore an intrinsic process of lobe building. The bifurcation of feeding channels is probably triggered when the gradient in the distributaries at the top of a lobe becomes flat and when turbidity currents find their way on the higher gradient on the lobe side. It may also be triggered by mass wasting on the lobe side. When a new lobe develops, the abandoned lobes continue to collect significant turbiditic deposits from the feeding channel spillover, so that the whole lobe complex remains active. A conceptual lithostratigraphic model is proposed for five morpho-sedimentary environments: lobe rims, lobe body, distributaries, levees, feeding channel. This study shows that high-resolution bathymetry ROV observations are necessary to fully understand the build-up processes of modern channel-mouth lobes.

Experimental study on the effects of fixed boundaries in channelized free surface dry granular flows

NASA Astrophysics Data System (ADS)

Sarno, Luca; Carleo, Luigi; Nicolina Papa, Maria

2017-04-01

The dynamics of granular mixtures, involved in geophysical flows like avalanches and debris flows, is far from being completely understood. Several features of their motion, such as rheological stratification, non-local and boundary effects, still represent open problems. Experimental investigations at laboratory scale are an important tool that can provide insights about the dynamics of gravity driven granular flows. The measuring techniques should be non-invasive in order to measure undisturbed flows. In this work we present an experimental campaign devoted to the measurement of the velocity profiles of free surface steady granular flows in an open channel. To achieve this goal the flows were recorded by two cameras and velocity profiles were obtained by image analysis. The employed granular medium consists of acetal-polymeric beads with a mean diameter of 3mm and an estimated internal friction angle of 27°. All the experiments have been performed in a 2m-long plexiglas flume with a rectangular cross-section and a slope angle of 30°. The upper part of the channel was used as a reservoir where the material was loaded before each run and then let flow down through an adjustable gate. Several mass flow rates were investigated. Three different basal surfaces were employed so as to observe slip and non-slip boundary conditions: a smooth Bakelite surface, a roughened surface, obtained by gluing a layer of grains on the bed surface and a sandpaper surface with characteristic length of the roughness equal to 425 µm. The flume is equipped with two high-speed cameras, one placed aside the channel and the other one perpendicular to the channel bed, as to get both side-wall and free surface velocity profiles. The particle image velocimetry open-source code, PIVlab, is employed for estimating the flow velocities. All the free surface velocity profiles show an approximately parabolic shape with a maximum at the cross-section midpoint and a minimum at the side-walls, due to the wall friction. Different kinds of side-wall velocity profiles are observed. As regards the smooth basal surface, a slip velocity at the bed is observed. The profiles are Bagnold-type near the free surface and become linear as the depth increases. On the glued-grain basal surface the flow velocity at the bed is null and all the velocity profiles show a rheological stratification with a lower exponential tail and an upper linear profile. Grain rolling is observed at the sandpaper bed, instead. With the increase of flow depths, the velocity profiles gradually shift from the ones observed on the smooth bed to the ones observed on the glued-grain bed. In order to further understand the constitutive behaviour of granular mixtures, it is useful to perform simultaneous measurements of flow velocity and volume fraction. In this perspective, a new series of experiments is actually undergoing for the measurement of the volume fraction.

Photolysis of Caged Ca2+ But Not Receptor-Mediated Ca2+ Signaling Triggers Astrocytic Glutamate Release

PubMed Central

Smith, Nathan A.; Xu, Qiwu; Goldman, Siri; Peng, Weiguo; Huang, Jason H.; Takano, Takahiro; Nedergaard, Maiken

2013-01-01

Astrocytes in hippocampal slices can dynamically regulate synaptic transmission in a process mediated by increases in intracellular Ca2+. However, it is debated whether astrocytic Ca2+ signals result in release of glutamate. We here compared astrocytic Ca2+ signaling triggered by agonist exposure versus photolysis side by side. Using transgenic mice in which astrocytes selectively express the MrgA1 receptor, we found that receptor-mediated astrocytic Ca2+ signaling consistently triggered neuronal hyperpolarization and decreased the frequency of miniature excitatory postsynaptic currents (EPSCs). In contrast, photolysis of caged Ca2+ (o-nitrophenyl–EGTA) in astrocytes led to neuronal depolarization and increased the frequency of mEPSCs through a metabotropic glutamate receptor-mediated pathway. Analysis of transgenic mice in which astrocytic vesicular release is suppressed (dominant-negative SNARE mice) and pharmacological manipulations suggested that glutamate is primarily released by opening of anion channels rather than exocytosis. Combined, these studies show that photolysis but not by agonists induced astrocytic Ca2+ signaling triggers glutamate release. PMID:24174673

Temperature Effects on the Optical Parameters of a Passively Q-Switched Diode-Side Pumped Yb,Er-Laser

NASA Astrophysics Data System (ADS)

Bogdanovich, M. V.; Izyneev, A. A.; Lantsov, K. I.; Lepchenkov, K. V.; Ryabtsev, A. G.; Pavlovskii, V. N.; Sadovskii, P. I.; Svitenkov, I. E.; Shchemelev, M. A.

2018-03-01

Temperature effects on photoluminescence and absorption spectra of the active medium (LGS-DE erbium phosphate glass) and passive Q-switch (MgAl2O4:Co2+ crystal) of a diode-side pumped Yb,Er-laser are studied. The obtained data are applied to an analysis of the spectral and energetic characteristics of compact erbium emitters. It is established that the dominant generation channel in the temperature range 233-328 K is the optical transition between lower Stark sublevels of Er3+ states 4I13/2 and 4I15/2 (λ = 1532.0-1533.9 nm). A rate-equation system taking into account thermal population of Stark sublevels of states 4I13/2 and 4I15/2 is proposed to describe the experimental temperature dependence of the threshold absorbed power of the pumping radiation. This system and the lasing threshold enable modeling of Yb,Er-emitter output energetic and temporal characteristics.

Bubble propagation on a rail: a concept for sorting bubbles by size

NASA Astrophysics Data System (ADS)

Franco-Gómez, Andrés; Thompson, Alice B.; Hazel, Andrew L.; Juel, Anne

We demonstrate experimentally that the introduction of a rail, a small height constriction, within the cross-section of a rectangular channel could be used as a robust passive sorting device in two-phase fluid flows. Single air bubbles carried within silicone oil are generally transported on one side of the rail. However, for flow rates marginally larger than a critical value, a narrow band of bubble sizes can propagate (stably) over the rail, while bubbles of other sizes segregate to the side of the rail. The width of this band of bubble sizes increases with flow rate and the size of the most stable bubble can be tuned by varying the rail width. We present a complementary theoretical analysis based on a depth-averaged theory, which is in qualitative agreement with the experiments. The theoretical study reveals that the mechanism relies on a non-trivial interaction between capillary and viscous forces that is fully dynamic, rather than being a simple modification of capillary static solutions.

Elysium Fossae

NASA Image and Video Library

2013-10-23

The channel shown here is part of a large system of depressions located on the eastern side of the Elysium Mons volcanic complex. The depression in this image from NASA 2001 Mars Odyssey spacecraft is located just south of Albor Tholus.

Origin and Dispersal History of Two Colonial Ascidian Clades in the Botryllus schlosseri Species Complex.

PubMed

Nydam, Marie L; Giesbrecht, Kirsten B; Stephenson, Emily E

2017-01-01

Human-induced global warming and species introductions are rapidly altering the composition and functioning of Earth's marine ecosystems. Ascidians (Phylum Chordata, Subphylum Tunicata, Class Ascidiacea) are likely to play an increasingly greater role in marine communities. The colonial ascidian B. schlosseri is a cryptic species complex comprising five genetically divergent clades (A-E). Clade A is a global species, and Clade E has so far been identified in European waters only. Using the largest mitochondrial cytochrome oxidase I datasets yet assembled, we determine the origin and dispersal history of these species. Nucleotide diversity and Approximate Bayesian Computation analyses support a Pacific origin for Clade A, with two likely dispersal scenarios that both show the northwestern Atlantic populations establishing early in the history of the species. Both Discrete Phylogeographic Analysis and Approximate Bayesian Computation support an origin of Clade E on the French side of the English Channel. An unsampled lineage evolved from the French lineage, which reflects the conclusion from the median joining network that not all Clade E lineages have been sampled. This unsampled lineage gave rise to the haplotypes on the English side of the English Channel, which were the ancestors to the Mediterranean and Bay of Biscay populations. Clade E has a wider geographic range than previously thought, and shows evidence of recent range expansion. Both Clade A and Clade E should be considered widespread species: Clade A globally and Clade E within Europe.

Seismic response of Pacific Park Plaza. I. Data and preliminary analysis

USGS Publications Warehouse

Celebi, M.; Safak, E.

1992-01-01

The objective of this paper is to present analyses of a set of acceleration response records obtained during the October 17, 1989 Loma Prieta earthquake (Ms = 7.1) from the 30-story, three-winged, ductile moment-resistant reinforced-concrete-framed Pacific Park Plaza Building, located in Emeryville, east of San Francisco, Calif. The building was constructed in 1983, and instrumented in 1985 with 21 channels of synchronized uniaxial accelerometers deployed throughout the structure, and three channels of accelerometers located at free-field outside on the north side of the building, all connected to a central recording system. In addition, a triaxial strong-motion accelerograph is deployed at free-field on the south side of the building. The predominant response modes of the building and the associated frequencies at approximately 0.4 Hz and 1.0 Hz are identified visually from the unprocessed records, and also from Fourier amplitude spectra of the processed records, which, as expected, reveal significant torsional motion. In addition, the response spectra of the free-field and basement motions are very similar. These spectra show that significant structural resonances at higher modes influence both the ground level and the free-field motions, thus rising the question as to the definition of free-field motion, at least at this site. This part of the paper includes the preliminary analyses of the data acquired from this building. Part 2 of the paper provides detailed analyses of the data using system identification techniques.

The therapeutic potential of Na+ and Ca2+ channel blockers in pain management.

PubMed

Sabido-David, Cibele; Faravelli, Laura; Salvati, Patricia

2004-10-01

Chronic pain affects a large percentage of the population, representing a socio-economic burden. Current treatments are characterised by suboptimal efficacy and/or side effects that limit their use. Among several approaches to treating chronic pain, voltage-sensitive Ca(2+) and Na(+) channels are promising targets. This review evaluates the preclinical evidence that supports the involvement of these targets, with specific attention to those subtypes that appear more strictly correlated with pain generation and sustainment, as well as those compounds that modulate the activity of Ca(2+) and/or Na(+) channels that are currently in clinical development for chronic pain conditions.

Means of increasing efficiency of CPC solar energy collector

DOEpatents

Chao, B.T.; Rabl, A.

1975-06-27

A device is provided for improving the thermal efficiency of a cylindrical radiant energy collector. A channel is placed next to and in close proximity to the nonreflective side of an energy reflective wall of a cylindrical collector. A coolant is piped through the channel and removes a portion of the nonreflective energy incident on the wall which is absorbed by the wall. The energy transferred to the coolant may be utilized in a useful manner.

Means of increasing efficiency of CPC solar energy collector

DOEpatents

Chao, Bei Tse; Rabl, Ari

1977-02-15

A device is provided for improving the thermal efficiency of a cylindrical radiant energy collector. A channel is placed next to and in close proximity to the nonreflective side of an energy reflective wall of a cylindrical collector. A coolant is piped through the channel and removes a portion of the nonreflective energy incident on the wall which is absorbed by the wall. The energy transferred to the coolant may be utilized in a useful manner.

Low hydrostatic head electrolyte addition to fuel cell stacks

DOEpatents

Kothmann, Richard E.

1983-01-01

A fuel cell and system for supply electrolyte, as well as fuel and an oxidant to a fuel cell stack having at least two fuel cells, each of the cells having a pair of spaced electrodes and a matrix sandwiched therebetween, fuel and oxidant paths associated with a bipolar plate separating each pair of adjacent fuel cells and an electrolyte fill path for adding electrolyte to the cells and wetting said matrices. Electrolyte is flowed through the fuel cell stack in a back and forth fashion in a path in each cell substantially parallel to one face of opposite faces of the bipolar plate exposed to one of the electrodes and the matrices to produce an overall head uniformly between cells due to frictional pressure drop in the path for each cell free of a large hydrostatic head to thereby avoid flooding of the electrodes. The bipolar plate is provided with channels forming paths for the flow of the fuel and oxidant on opposite faces thereof, and the fuel and the oxidant are flowed along a first side of the bipolar plate and a second side of the bipolar plate through channels formed into the opposite faces of the bipolar plate, the fuel flowing through channels formed into one of the opposite faces and the oxidant flowing through channels formed into the other of the opposite faces.

The effect of passive mixing on pressure drop and oxygen mass fraction using opposing channel flow field design in a Proton Exchange Membrane Fuel Cell

NASA Astrophysics Data System (ADS)

Singh, Anant Bir

This study investigates a flow field with opposing channel design. Previous studies on flow field designs have been focused on improving fuel utilization which often leads to increased pressure drop. This increased pressure drop is typical because standard designs employ either a single flow channel to clear blockages or dead end condition to force the flow through the gas diffusion layer. The disadvantage with these designs is the increased resistance to the flow which requires higher pressure, which becomes a parasitic loss that lowers the system efficiency. For this study the focus was to reduce the pressure drop by providing a less resistive path to the flow. To achieve a less resistive path, the inlet channel was split into two opposing channels. These channels are then recombined only to be split again for the next leg. Therefore, the split channel design should reduce the pressure drop which reduces the parasitic load and ultimately contributes to higher system efficiency. In addition the recombining of the streams at each leg should induce mixing. Having opposing channels should also increase cross flow under the lands to reduce mass transfer loses. The cathode side of the fuel cell is especially sensitive to the mass transport losses since air (oxygen mixed with nitrogen) is used for supplying oxygen unlike the anode side which uses pure hydrogen. To test the hypothesis of having benefits from an opposing channel design, both an experimental and analytical approach was taken. For the experiment, a serpentine flow field and opposing channel flow field plates were compared over several flow rates with compressed air. To test the hypothesis of increased mass transfer, the two flow fields were modeled using a CFD software package, COMSOL. It was found that the opposing channel configuration for high flow rate with multiple entry and exit conditions exhibited significant improvement over the single serpentine channel. Pressure drop was ⅓ less than the serpentine channel with similar conditions. Simulations for mass transfer show that recombining of the flow streams generate more uniform current density unlike the serpentine configuration where the current density was concentrated at the entrance of the flow stream. The background section provides a brief overview of the governing equations, the theory of flow field operation and previous bodies of work on flow field design. Recommendations are made for further verification of the design using a real working cell based on the results.

Approximate reversibility in the context of entropy gain, information gain, and complete positivity

NASA Astrophysics Data System (ADS)

Buscemi, Francesco; Das, Siddhartha; Wilde, Mark M.

2016-06-01

There are several inequalities in physics which limit how well we can process physical systems to achieve some intended goal, including the second law of thermodynamics, entropy bounds in quantum information theory, and the uncertainty principle of quantum mechanics. Recent results provide physically meaningful enhancements of these limiting statements, determining how well one can attempt to reverse an irreversible process. In this paper, we apply and extend these results to give strong enhancements to several entropy inequalities, having to do with entropy gain, information gain, entropic disturbance, and complete positivity of open quantum systems dynamics. Our first result is a remainder term for the entropy gain of a quantum channel. This result implies that a small increase in entropy under the action of a subunital channel is a witness to the fact that the channel's adjoint can be used as a recovery map to undo the action of the original channel. We apply this result to pure-loss, quantum-limited amplifier, and phase-insensitive quantum Gaussian channels, showing how a quantum-limited amplifier can serve as a recovery from a pure-loss channel and vice versa. Our second result regards the information gain of a quantum measurement, both without and with quantum side information. We find here that a small information gain implies that it is possible to undo the action of the original measurement if it is efficient. The result also has operational ramifications for the information-theoretic tasks known as measurement compression without and with quantum side information. Our third result shows that the loss of Holevo information caused by the action of a noisy channel on an input ensemble of quantum states is small if and only if the noise can be approximately corrected on average. We finally establish that the reduced dynamics of a system-environment interaction are approximately completely positive and trace preserving if and only if the data processing inequality holds approximately.

Single-channel measurements of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli

PubMed Central

Giri, Janhavi; Tang, John M.; Wirth, Christophe; Peneff, Caroline M.

2012-01-01

NanC is an Escherichia coli outer membrane protein involved in sialic acid (Neu5Ac, i.e., N-acetylneuraminic acid) uptake. Expression of the NanC gene is induced and controlled by Neu5Ac. The transport mechanism of Neu5Ac is not known. The structure of NanC was recently solved (PDB code: 2WJQ) and includes a unique arrangement of positively charged (basic) side chains consistent with a role in acidic sugar transport. However, initial functional measurements of NanC failed to find its role in the transport of sialic acids, perhaps because of the ionic conditions used in the experiments. We show here that the ionic conditions generally preferred for measuring the function of outer-membrane porins are not appropriate for NanC. Single channels of NanC at pH 7.0 have: (1) conductance 100 pS to 800 pS in 100 mM KCl to 3 M KCl), (2) anion over cation selectivity (Vreversal = +16 mV in 250 mM KCl || 1 M KCl), and (3) two forms of voltage-dependent gating (channel closures above ±200 mV). Single-channel conductance decreases by 50% when HEPES concentration is increased from 100 μM to 100 mM in 250 mM KCl at pH 7.4, consistent with the two HEPES binding sites observed in the crystal structure. Studying alternative buffers, we find that phosphate interferes with the channel conductance. Single-channel conductance decreases by 19% when phosphate concentration is increased from 0 mM to 5 mM in 250 mM KCl at pH 8.0. Surprisingly, TRIS in the baths reacts with Ag|AgCl electrodes, producing artifacts even when the electrodes are on the far side of agar–KCl bridges. A suitable baseline solution for NanC is 250 mM KCl adjusted to pH 7.0 without buffer. PMID:22246445

Application of the Shiono and Knight Method in asymmetric compound channels with different side slopes of the internal wall

NASA Astrophysics Data System (ADS)

Alawadi, Wisam; Al-Rekabi, Wisam S.; Al-Aboodi, Ali H.

2018-03-01

The Shiono and Knight Method (SKM) is widely used to predict the lateral distribution of depth-averaged velocity and boundary shear stress for flows in compound channels. Three calibrating coefficients need to be estimated for applying the SKM, namely eddy viscosity coefficient ( λ), friction factor ( f) and secondary flow coefficient ( k). There are several tested methods which can satisfactorily be used to estimate λ, f. However, the calibration of secondary flow coefficients k to account for secondary flow effects correctly is still problematic. In this paper, the calibration of secondary flow coefficients is established by employing two approaches to estimate correct values of k for simulating asymmetric compound channel with different side slopes of the internal wall. The first approach is based on Abril and Knight (2004) who suggest fixed values for main channel and floodplain regions. In the second approach, the equations developed by Devi and Khatua (2017) that relate the variation of the secondary flow coefficients with the relative depth ( β) and width ratio ( α) are used. The results indicate that the calibration method developed by Devi and Khatua (2017) is a better choice for calibrating the secondary flow coefficients than using the first approach which assumes a fixed value of k for different flow depths. The results also indicate that the boundary condition based on the shear force continuity can successfully be used for simulating rectangular compound channels, while the continuity of depth-averaged velocity and its gradient is accepted boundary condition in simulations of trapezoidal compound channels. However, the SKM performance for predicting the boundary shear stress over the shear layer region may not be improved by only imposing the suitable calibrated values of secondary flow coefficients. This is because difficulties of modelling the complex interaction that develops between the flows in the main channel and on the floodplain in this region.

Electrokinetic sample preconcentration and hydrodynamic sample injection for microchip electrophoresis using a pneumatic microvalve.

PubMed

Cong, Yongzheng; Katipamula, Shanta; Geng, Tao; Prost, Spencer A; Tang, Keqi; Kelly, Ryan T

2016-02-01

A microfluidic platform was developed to perform online electrokinetic sample preconcentration and rapid hydrodynamic sample injection for zone electrophoresis using a single microvalve. The polydimethylsiloxane microchip comprises a separation channel, a side channel for sample introduction, and a control channel which is used as a pneumatic microvalve aligned at the intersection of the two flow channels. The closed microvalve, created by multilayer soft lithography, serves as a nanochannel preconcentrator under an applied electric potential, enabling current to pass through while preventing bulk flow. Once analytes are concentrated, the valve is briefly opened and the stacked sample is pressure injected into the separation channel for electrophoretic separation. Fluorescently labeled peptides were enriched by a factor of ∼450 in 230 s. This method enables both rapid analyte concentration and controlled injection volume for high sensitivity, high-resolution CE. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Characterization of the human pH- and PKA-activated ClC-2G(2 alpha) Cl- channel.

PubMed

Sherry, A M; Stroffekova, K; Knapp, L M; Kupert, E Y; Cuppoletti, J; Malinowska, D H

1997-08-01

A ClC-2G(2 alpha) Cl- channel was identified to be present in human lung and stomach, and a partial cDNA for this Cl- channel was cloned from a human fetal lung library. A full-length expressible human ClC-2G(2 alpha) cDNA was constructed by ligation of mutagenized expressible rabbit ClC-2G(2 alpha) cDNA with the human lung ClC-2G(2 alpha) cDNA, expressed in oocytes, and characterized at the single-channel level. Adenosine 3',5'-cyclic monophosphate-dependent protein kinase (PKA) treatment increased the probability of opening of the channel (Po). After PKA activation, the channel exhibited a linear (r = 0.99) current-voltage curve with a slope conductance of 22.1 +/- 0.8 pS in symmetric 800 mM tetraethylammonium chloride (TEACl; pH 7.4). Under fivefold gradient conditions of TEACl, a reversal potential of +21.5 +/- 2.8 mV was measured demonstrating anion-to-cation discrimination. As previously demonstrated for the rabbit ClC-2G(2 alpha) Cl- channel, the human analog, hClC-2G(2 alpha), was active at pH 7.4 as well as when the pH of the extracellular face of the channel (trans side of the bilayer; pHtrans) was asymmetrically reduced to pH 3.0. The extent of PKA activation was dependent on pHtrans. With PKA treatment, Po increased fourfold with a pHtrans of 7.4 and eightfold with a pHtrans of 3.0. Effects of sequential PKA addition followed by pHtrans reduction on the same channel suggested that the PKA- and pH-dependent increases in channel Po were separable and cumulative. Northern analysis showed ClC-2G(2 alpha) mRNA to be present in human adult and fetal lung and adult stomach, and quantitative reverse transcriptase-polymerase chain reaction showed this channel to be present in the adult human lung and stomach at about one-half the level found in fetal lung. The findings of the present study suggest that the ClC-2G(2 alpha) Cl- channel may play an important role in Cl- transport in the fetal and adult human lung.

Geomorphic features off southern California as seen by GLORIA side-scan sonar system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Edwards, B.D.; Field, M.E.; Carlson, P.R.

1985-02-01

Approximately 165,000 km/sup 2/ of the sea floor off southern California was mapped during May 1984, as part of a USGS/IOS cooperative program to study the newly proclaimed Exclusive Economic Zone (EEZ) of the US Pacific margin. The area was insonified using the Geological Long-Range Inclined Asdic (GLORIA), a long-range side-scan sonar system. Images were corrected for water-column velocity anomalies, for along-track distortions caused by acoustic ray travel paths. A photomosaic of the overlapping sonographs has been compiled at a scale of 1:375,000. The basins of the inner California continental borderland are characterized by both sinuous channel and fan complexesmore » and by feathery acoustic patterns indicating active sediment transport. In contrast, outer borderland basins appear to be more sediment starved, exhibit large areas of sediment failure, and show significant structural influence. West of Patton Escarpment, the sonographs are dominated by acoustic patterns showing volcanic ridges and seamounts and by deposits of the Monterey and Arguello fans. Arguello fan, for example, exhibits multiple sinuous channels that have transported sediment 60 km south from the canyon mouth. These channels coalesce into a single 100-km long, westward-meandering channel that terminates in a 600-m deep box canyon. A zone of sediment failure is identifiable on the north levee of an upper fan channel. Tectonic trends associated with oceanic basement are highlighted by the terminus of the west-trending Murray Fracture Zone and by the prevailing northeast trend of volcanic ridge and seamount chains.« less

An inhibitor of TRPV1 channels isolated from funnel Web spider venom.

PubMed

Kitaguchi, Tetsuya; Swartz, Kenton J

2005-11-29

Capsaicin receptor channels (TRPV1) are nonselective cation channels that integrate multiple noxious stimuli in sensory neurons. In an effort to identify new inhibitors of these channels we screened a venom library for activity against TRPV1 channels and found robust inhibitory activity in venom from Agelenopsis aperta, a north American funnel web spider. Fractionation of the venom using reversed-phase HPLC resulted in the purification of two acylpolyamine toxins, AG489 and AG505, which inhibit TRPV1 channels from the extracellular side of the membrane. The activity of AG489 was characterized further, and the toxin was found to inhibit TRPV1 channels with a K(i) of 0.3 microM at -40 mV. Inhibition of TRPV1 channels by AG489 is strongly voltage-dependent, with relief of inhibition at positive voltages, consistent with the toxin inhibiting the channel through a pore-blocking mechanism. We used scanning mutagenesis throughout the TM5-TM6 linker, a region thought to form the outer pore of TRPV1 channels, to identify pore mutations that alter toxin affinity. Four mutants dramatically decrease toxin affinity and several mutants increase toxin affinity, consistent with the notion that the TM5-TM6 linker forms the outer vestibule of TRPV1 channels and that AG489 is a pore blocker.

Chitosugar translocation by an unexpressed monomeric protein channel

NASA Astrophysics Data System (ADS)

Soysa, H. Sasimali M.; Suginta, Wipa; Moonsap, Watcharaporn; Smith, M. F.

2018-05-01

The outer membrane protein channel Ec ChiP , associated with a silent gene in E . coli, is a monomeric chitoporin. In a glucose-deficient environment, E . coli can express the ChiP gene to exploit chitin degradation products. Single-channel small ion current measurements, which reveal the dynamics of single sugar molecules trapped in channel, are used here to study the exotic transport of chitosugars by E . coli. Molecules escape from the channel on multiple timescales. Voltage-dependent trapping rates observed for charged chitosan molecules, as well as model calculations, indicate that the rapid escape processes are those in which the molecule escapes back to the side of the membrane from which it originated. The probability that a sugar molecule is translocated through the membrane is thus estimated from the current data and the dependence of this translocation probability on the length of the chitosugar molecule and the applied voltage analyzed. The described method for obtaining the translocation probability and related molecular translocation current is applicable to other transport channels.

Voltage-sensing domain of voltage-gated proton channel Hv1 shares mechanism of block with pore domains.

PubMed

Hong, Liang; Pathak, Medha M; Kim, Iris H; Ta, Dennis; Tombola, Francesco

2013-01-23

Voltage-gated sodium, potassium, and calcium channels are made of a pore domain (PD) controlled by four voltage-sensing domains (VSDs). The PD contains the ion permeation pathway and the activation gate located on the intracellular side of the membrane. A large number of small molecules are known to inhibit the PD by acting as open channel blockers. The voltage-gated proton channel Hv1 is made of two VSDs and lacks the PD. The location of the activation gate in the VSD is unknown and open channel blockers for VSDs have not yet been identified. Here, we describe a class of small molecules which act as open channel blockers on the Hv1 VSD and find that a highly conserved phenylalanine in the charge transfer center of the VSD plays a key role in blocker binding. We then use one of the blockers to show that Hv1 contains two intracellular and allosterically coupled gates. Copyright © 2013 Elsevier Inc. All rights reserved.

Low Voltage Activated Calcium Channels - Their Role in HER2 Driven Breast Cancer and Potential as a New Therapeutic Target

DTIC Science & Technology

2016-10-01

combined with chemotherapy , but chemotherapy causes undesirable side effects due to off-target effects on normal tissue, which diminishes quality of life...highest response rates when combined with chemotherapy , but chemotherapy causes undesirable side effects due to off-target effects on normal...patients. Therefore, the overall goal of this proposal is to develop a tumor-specific, safe and effective therapy for breast cancer. We concentrate on

[A study of urine concentrating mechanism--a molecular biological approach].

PubMed

Marumo, F

1994-07-01

Human urine can be concentrated up to four times higher than that of the plasma. Urine concentrating mechanism has attracted for a long time. However, studies in the field are now picking up momentum due to recent breakthrough discoveries using molecular biology techniques. Vasopressin-regulated water channel in the apical membrane of the collecting duct and water channel in the basolateral side of the membrane were cloned. cloned. Osmolality-dependent chloride channel in the thin ascending limb of Henle was also cloned. In addition, vasopressin-regulated urea transporter was found in the collecting duct. These newly discovered channels and transporter should be playing important physiological roles in urine concentrating mechanism. Furthermore, recent findings on osmolytes and their transporters also add to the list of urine concentrating mechanisms.

The theoretical simulation on electrostatic distribution of 1st proximity region in proximity focusing low-light-level image intensifier

NASA Astrophysics Data System (ADS)

Zhang, Liandong; Bai, Xiaofeng; Song, De; Fu, Shencheng; Li, Ye; Duanmu, Qingduo

2015-03-01

Low-light-level night vision technology is magnifying low light level signal large enough to be seen by naked eye, which uses the photons - photoelectron as information carrier. Until the micro-channel plate was invented, it has been possibility for the realization of high performance and miniaturization of low-light-level night vision device. The device is double-proximity focusing low-light-level image intensifier which places a micro-channel plate close to photocathode and phosphor screen. The advantages of proximity focusing low-light-level night vision are small size, light weight, small power consumption, no distortion, fast response speed, wide dynamic range and so on. It is placed parallel to each other for Micro-channel plate (both sides of it with metal electrode), the photocathode and the phosphor screen are placed parallel to each other. The voltage is applied between photocathode and the input of micro-channel plate when image intensifier works. The emission electron excited by photo on the photocathode move towards to micro-channel plate under the electric field in 1st proximity focusing region, and then it is multiplied through the micro-channel. The movement locus of emission electrons can be calculated and simulated when the distributions of electrostatic field equipotential lines are determined in the 1st proximity focusing region. Furthermore the resolution of image tube can be determined. However the distributions of electrostatic fields and equipotential lines are complex due to a lot of micro-channel existing in the micro channel plate. This paper simulates electrostatic distribution of 1st proximity region in double-proximity focusing low-light-level image intensifier with the finite element simulation analysis software Ansoft maxwell 3D. The electrostatic field distributions of 1st proximity region are compared when the micro-channel plates' pore size, spacing and inclination angle ranged. We believe that the electron beam movement trajectory in 1st proximity region will be better simulated when the electronic electrostatic fields are simulated.

Side wire feed for welding apparatus

NASA Technical Reports Server (NTRS)

Arnett, J. C.

1974-01-01

Coaxial electrode arrangement has solid central electrode, insulated outer electrode, and transverse channel for feeding wire through tip of electrode assembly. Polymeric insulation is thrust aside by pressure, which is provided by separately operated mechanism acting through central electrode.

27. A VIEW TOWARD THE FISHING PIER AT THE EAST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. A VIEW TOWARD THE FISHING PIER AT THE EAST END OF THE NORTH TRAINING WALL, SHOWING SIDE WALL CONSTRUCTION. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

Part 1 - Experimental study of the pressure fluctuations on propeller turbine runner blades during steady-state operation

NASA Astrophysics Data System (ADS)

Houde, S.; Fraser, R.; Ciocan, G. D.; Deschênes, C.

2012-11-01

A good evaluation of the unsteady pressure field on hydraulic turbine blades is critical in evaluating the turbine lifespan and its maintenance schedule. Low-head turbines such as Kaplan and Propeller, using a relatively low number of blades supported only at the hub, may also undergo significant deflections at the blade tips which will lead to higher amplitude vibration compared to Francis turbines. Furthermore, the precise evaluation of the unsteady pressure distribution on low-head turbines is still a challenge for computational fluid dynamics (CFD). Within the framework of an international research consortium on low-head turbines, a research project was instigated at the Hydraulic Machines Laboratory in Laval University (LAMH) to perform experimental measurements of the unsteady pressure field on propeller turbine model runner blades. The main objective of the project was to measure the pressure fluctuations on a wide band of frequencies, both in a blade-to-blade channel and on the pressure and suction side of the same blade, to provide validation data for CFD computations. To do so, a 32 channels telemetric data transmission system was used to extract the signal of 31 pressure transducers and two strain gages from the rotating part at an acquisition frequency of 5 KHz. The miniature piezoelectric pressure transducers were placed on two adjacent runner blades according to an estimated pressure distribution coming from flow simulations. Two suction sides and one pressure side were instrumented. The strain gages were mounted in full-bridge on both pressure and suction sides to measure the blade span wise deflection. In order to provide boundary conditions for flow simulations, the test bench conditions during the measurements were acquired. The measurements were made in different operating conditions ranging from part load, where a cavitating vortex occurs, to full load under different heads. The results enabled the identification and the quantification of the major known sources of pressure fluctuation as well as some unexpected ones. The paper first presents the experimental methodology discussing relevant topics such as telemetric system setup, transducers calibration and errors analysis. The main results are then presented to illustrate the relative amplitude of the main source of pressure fluctuations under different operating conditions. The discussion and conclusion addresses the important observations stemming from the data analysis and illustrates that most of the results can be correlated with the known behavior of hydraulic turbines while some require further investigation.

Loss-tolerant measurement-device-independent quantum private queries

NASA Astrophysics Data System (ADS)

Zhao, Liang-Yuan; Yin, Zhen-Qiang; Chen, Wei; Qian, Yong-Jun; Zhang, Chun-Mei; Guo, Guang-Can; Han, Zheng-Fu

2017-01-01

Quantum private queries (QPQ) is an important cryptography protocol aiming to protect both the user’s and database’s privacy when the database is queried privately. Recently, a variety of practical QPQ protocols based on quantum key distribution (QKD) have been proposed. However, for QKD-based QPQ the user’s imperfect detectors can be subjected to some detector- side-channel attacks launched by the dishonest owner of the database. Here, we present a simple example that shows how the detector-blinding attack can damage the security of QKD-based QPQ completely. To remove all the known and unknown detector side channels, we propose a solution of measurement-device-independent QPQ (MDI-QPQ) with single- photon sources. The security of the proposed protocol has been analyzed under some typical attacks. Moreover, we prove that its security is completely loss independent. The results show that practical QPQ will remain the same degree of privacy as before even with seriously uncharacterized detectors.

Measurement-device-independent quantum coin tossing

NASA Astrophysics Data System (ADS)

Zhao, Liangyuan; Yin, Zhenqiang; Wang, Shuang; Chen, Wei; Chen, Hua; Guo, Guangcan; Han, Zhengfu

2015-12-01

Quantum coin tossing (QCT) is an important primitive of quantum cryptography and has received continuous interest. However, in practical QCT, Bob's detectors can be subjected to detector-side channel attacks launched by dishonest Alice, which will possibly make the protocol completely insecure. Here, we report a simple strategy of a detector-blinding attack based on a recent experiment. To remove all the detector side channels, we present a solution of measurement-device-independent QCT (MDI-QCT). This method is similar to the idea of MDI quantum key distribution (QKD). MDI-QCT is loss tolerant with single-photon sources and has the same bias as the original loss-tolerant QCT under a coherent attack. Moreover, it provides the potential advantage of doubling the secure distance for some special cases. Finally, MDI-QCT can also be modified to fit the weak coherent-state sources. Thus, based on the rapid development of practical MDI-QKD, our proposal can be implemented easily.

Loss-tolerant measurement-device-independent quantum private queries.

PubMed

Zhao, Liang-Yuan; Yin, Zhen-Qiang; Chen, Wei; Qian, Yong-Jun; Zhang, Chun-Mei; Guo, Guang-Can; Han, Zheng-Fu

2017-01-04

Quantum private queries (QPQ) is an important cryptography protocol aiming to protect both the user's and database's privacy when the database is queried privately. Recently, a variety of practical QPQ protocols based on quantum key distribution (QKD) have been proposed. However, for QKD-based QPQ the user's imperfect detectors can be subjected to some detector- side-channel attacks launched by the dishonest owner of the database. Here, we present a simple example that shows how the detector-blinding attack can damage the security of QKD-based QPQ completely. To remove all the known and unknown detector side channels, we propose a solution of measurement-device-independent QPQ (MDI-QPQ) with single- photon sources. The security of the proposed protocol has been analyzed under some typical attacks. Moreover, we prove that its security is completely loss independent. The results show that practical QPQ will remain the same degree of privacy as before even with seriously uncharacterized detectors.

Robot Arm with Tendon Connector Plate and Linear Actuator

NASA Technical Reports Server (NTRS)

Bridgwater, Lyndon (Inventor); Millerman, Alexander (Inventor); Ihrke, Chris A. (Inventor); Diftler, Myron A. (Inventor); Nguyen, Vienny (Inventor)

2014-01-01

A robotic system includes a tendon-driven end effector, a linear actuator, a flexible tendon, and a plate assembly. The linear actuator assembly has a servo motor and a drive mechanism, the latter of which translates linearly with respect to a drive axis of the servo motor in response to output torque from the servo motor. The tendon connects to the end effector and drive mechanism. The plate assembly is disposed between the linear actuator assembly and the tendon-driven end effector and includes first and second plates. The first plate has a first side that defines a boss with a center opening. The second plate defines an accurate through-slot having tendon guide channels. The first plate defines a through passage for the tendon between the center opening and a second side of the first plate. A looped end of the flexible tendon is received within the tendon guide channels.

Magnetic translator bearings

NASA Technical Reports Server (NTRS)

Hockney, Richard L. (Inventor); Downer, James R. (Inventor); Eisenhaure, David B. (Inventor); Hawkey, Timothy J. (Inventor); Johnson, Bruce G. (Inventor)

1990-01-01

A magnetic bearing system for enabling translational motion includes a carriage and a shaft for movably supporting the carriage; a first magnetic bearing fixed to one of the carriage and shaft and slidably received in a first channel of the other of the carriage and shaft. The first channel is generally U shaped with two side walls and a back wall. The magnetic bearing includes a pair of spaced magnetic pole pieces, each pole piece having a pair of electromagnetic coils mounted on poles on opposite ends of the pole piece proximate the side walls, and a third electromagnetic coil mounted on a pole of the pole piece proximate the backwall; a motion sensor for sensing translational motion along two axes and rotationally about three axes of the carriage and shaft relative to each other; and a correction circuit responsive to the sensor for generating a correction signal to drive the coils to compensate for any misalignment sensed between the carriage and the shaft.

Three-Dimensional Transport Modeling for Proton Exchange Membrane(PEM) Fuel Cell with Micro Parallel Flow Field

PubMed Central

Lee, Pil Hyong; Han, Sang Seok; Hwang, Sang Soon

2008-01-01

Modeling and simulation for heat and mass transport in micro channel are being used extensively in researches and industrial applications to gain better understanding of the fundamental processes and to optimize fuel cell designs before building a prototype for engineering application. In this study, we used a single-phase, fully three dimensional simulation model for PEMFC that can deal with both anode and cathode flow field for examining the micro flow channel with electrochemical reaction. The results show that hydrogen and oxygen were solely supplied to the membrane by diffusion mechanism rather than convection transport, and the higher pressure drop at cathode side is thought to be caused by higher flow rate of oxygen at cathode. And it is found that the amount of water in cathode channel was determined by water formation due to electrochemical reaction plus electro-osmotic mass flux directing toward the cathode side. And it is very important to model the back diffusion and electro-osmotic mass flux accurately since the two flux was closely correlated each other and greatly influenced for determination of ionic conductivity of the membrane which directly affects the performance of fuel cell. PMID:27879774

Layered Wyner-Ziv video coding.

PubMed

Xu, Qian; Xiong, Zixiang

2006-12-01

Following recent theoretical works on successive Wyner-Ziv coding (WZC), we propose a practical layered Wyner-Ziv video coder using the DCT, nested scalar quantization, and irregular LDPC code based Slepian-Wolf coding (or lossless source coding with side information at the decoder). Our main novelty is to use the base layer of a standard scalable video coder (e.g., MPEG-4/H.26L FGS or H.263+) as the decoder side information and perform layered WZC for quality enhancement. Similar to FGS coding, there is no performance difference between layered and monolithic WZC when the enhancement bitstream is generated in our proposed coder. Using an H.26L coded version as the base layer, experiments indicate that WZC gives slightly worse performance than FGS coding when the channel (for both the base and enhancement layers) is noiseless. However, when the channel is noisy, extensive simulations of video transmission over wireless networks conforming to the CDMA2000 1X standard show that H.26L base layer coding plus Wyner-Ziv enhancement layer coding are more robust against channel errors than H.26L FGS coding. These results demonstrate that layered Wyner-Ziv video coding is a promising new technique for video streaming over wireless networks.

Keep-Left Behavior Induced by Asymmetrically Profiled Walls

NASA Astrophysics Data System (ADS)

Oliveira, C. L. N.; Vieira, A. P.; Helbing, D.; Andrade, J. S.; Herrmann, H. J.

2016-01-01

We show, computationally and analytically, that asymmetrically shaped walls can organize the flow of pedestrians driven in opposite directions through a corridor. Precisely, a two-lane ordered state emerges in which people always walk on the left-hand side (or right-hand side), controlled by the system's parameters. This effect depends on features of the channel geometry, such as the asymmetry of the profile and the channel width, as well as on the density and the drift velocity of pedestrians, and the intensity of noise. We investigate in detail the influence of these parameters on the flow and discover a crossover between ordered and disordered states. Our results show that an ordered state only appears within a limited range of drift velocities. Moreover, increasing noise may suppress such flow organization, but the flow is always sustained. This is in contrast with the "freezing by heating" phenomenon according to which pedestrians tend to clog in smooth channels for strong noise [Phys. Rev. Lett. 84, 1240 (2000)]. Therefore, the ratchetlike effect proposed here acts on the system not only to induce a "keep-left" behavior but also to prevent the freezing by heating clogging phenomenon. Besides pedestrian flow, this new phenomenon has other potential applications in microfluidics systems.

CFD analyses of coolant channel flowfields

NASA Technical Reports Server (NTRS)

Yagley, Jennifer A.; Feng, Jinzhang; Merkle, Charles L.

1993-01-01

The flowfield characteristics in rocket engine coolant channels are analyzed by means of a numerical model. The channels are characterized by large length to diameter ratios, high Reynolds numbers, and asymmetrical heating. At representative flow conditions, the channel length is approximately twice the hydraulic entrance length so that fully developed conditions would be reached for a constant property fluid. For the supercritical hydrogen that is used as the coolant, the strong property variations create significant secondary flows in the cross-plane which have a major influence on the flow and the resulting heat transfer. Comparison of constant and variable property solutions show substantial differences. In addition, the property variations prevent fully developed flow. The density variation accelerates the fluid in the channels increasing the pressure drop without an accompanying increase in heat flux. Analyses of the inlet configuration suggest that side entry from a manifold can affect the development of the velocity profile because of vortices generated as the flow enters the channel. Current work is focused on studying the effects of channel bifurcation on the flow field and the heat transfer characteristics.