An overview of EMP effects and their control

NASA Astrophysics Data System (ADS)

Culligan, A. J.

1985-09-01

The environments associated with a nuclear detonation are probably the most extreme of all the environments to which an electronic equipment system may be exposed. One of these environments is related to the electromagnetic pulse (EMP) which represents an intense pulse of radiated electromagnetic energy. The endo-atmospheric EMP is generally not considered a major threat in comparison with other nuclear weapon effects related to blast, thermal phenomena, neutrons, and gamma rays. The situation is different with respect to the exo-atmospheric EMP which has the potential to simultaneously damage communication and allied networks over many thousands of square miles. The present paper is concerned with these EMP effects and the possibilities for protecting equipment from EMP. For such a protection, an understanding of the system's electronic topology is essential, and component/circuit susceptibility levels must be specified. Attention is given to the wire grid model of a strike aircraft, and the simulation of the EMP environment.

Parallel-plate transmission line type of EMP simulators: Systematic review and recommendations

NASA Astrophysics Data System (ADS)

Giri, D. V.; Liu, T. K.; Tesche, F. M.; King, R. W. P.

1980-05-01

This report presents various aspects of the two-parallel-plate transmission line type of EMP simulator. Much of the work is the result of research efforts conducted during the last two decades at the Air Force Weapons Laboratory, and in industries/universities as well. The principal features of individual simulator components are discussed. The report also emphasizes that it is imperative to hybridize our understanding of individual components so that we can draw meaningful conclusions of simulator performance as a whole.

Study of storm surge trends in typhoon-prone coastal areas based on observations and surge-wave coupled simulations

NASA Astrophysics Data System (ADS)

Feng, Xingru; Li, Mingjie; Yin, Baoshu; Yang, Dezhou; Yang, Hongwei

2018-06-01

This is a study of the storm surge trends in some of the typhoon-prone coastal areas of China. An unstructured-grid, storm surge-wave-tide coupled model was established for the coastal areas of Zhejiang, Fujian and Guangdong provinces. The coupled model has a high resolution in coastal areas, and the simulated results compared well with the in situ observations and satellite altimeter data. The typhoon-induced storm surges along the coast of the study areas were simulated based on the established coupled model for the past 20 years (1997-2016). The simulated results were used to analyze the trends of the storm surges in the study area. The extreme storm surge trends along the central coast of Fujian Province reached up to 0.06 m/y, significant at the 90% confidence level. The duration of the storm surges greater than 1.0 and 0.7 m had an increasing trend along the coastal area of northern Fujian Province, significant at confidence levels of 70%-91%. The simulated trends of the extreme storm surges were also validated by observations from two tide gauge stations. Further studies show that the correlation coefficient (RTE) between the duration of the storm surge greater than 1 m and the annual ENSO index can reach as high as 0.62, significant at the 99% confidence level. This occurred in a location where the storm surge trend was not significant. For the areas with significant increasing storm surge trends, RTE was small and not significant. This study identified the storm surge trends for the full complex coastline of the study area. These results are useful both for coastal management by the government and for coastal engineering design.

Electromagnetic pulse (EMP) radiation by laser interaction with a solid H2 ribbon

NASA Astrophysics Data System (ADS)

De Marco, M.; Krása, J.; Cikhardt, J.; Velyhan, A.; Pfeifer, M.; Dudžák, R.; Dostál, J.; Krouský, E.; Limpouch, J.; Pisarczyk, T.; Kalinowska, Z.; Chodukowski, T.; Ullschmied, J.; Giuffrida, L.; Chatain, D.; Perin, J.-P.; Margarone, D.

2017-08-01

The electromagnetic pulses (EMPs) generated during the interaction of a focused 1.315-μm sub-nanosecond laser pulse with a solid hydrogen ribbon were measured. The strength and temporal characteristics of EMPs were found to be dependent on the target density. If a low density target is ionized during the interaction with the laser, and the plasma does not physically touch the target holder, the EMP is weaker in strength and shorter in time duration. It is shown that during the H2 target experiment, the EMP does not strongly affect the response of fast electronic devices. The measurements of the EMP were carried out by Rohde&Schwarz B-Probes, particularly sensitive in the frequency range from 30 MHz and 1 GHz. Numerical simulations of resonant frequencies of the target chamber used in the experiment at the Prague Asterix Laser System kJ-class laser facility elucidate the peaked structure of EMP frequency spectra in the GHz domain.

Effect of hurricane paths on storm surge response at Tianjin, China

NASA Astrophysics Data System (ADS)

Feng, Xingru; Yin, Baoshu; Yang, Dezhou

2012-06-01

A hurricane induced storm surge simulation system was developed for Tianjin coast, which consists of a hurricane model and a storm surge model. The peak storm surge result of the simulation agreed well with that of the observation. Three observed paths (Rita, Mimie and WINNIE) and a hypothetical path (Rita2) were chosen as the selective hurricane paths according to their positions relative to Tianjin. The sensitivity of Tianjin storm surge to the four paths was investigated using the validated storm surge simulation system. Three groups of experiments were done. In group one, the models were forced by the wind field and air pressure; in group two and three the models were forced by the wind only and the air pressure only respectively. In the experiments, the hurricane moved with a fixed speed and an intensity of 50 year return period. The simulation results show that path of the type Rita2 is the easiest to cause storm surge disaster in Tianjin, and the effect of air pressure forcing is most evident for path of the type Rita in Tianjin storm surge process. The above conclusions were analyzed through the evolution of the wind fields and the air pressure distributions. Comparing the experiment results of Group one, two and three, it can be seen that the storm surge is mainly induced by the wind forcing and the nonlinear interaction between the effect of wind forcing and air pressure forcing on the storm surge tends to weaken the storm surge.

Radar cross-section measurements and simulation of a tethered satellite. The small expendable deployer system end-mass payload

NASA Technical Reports Server (NTRS)

Cravey, Robin L.; Fralick, Dion T.; Vedeler, Erik

1995-01-01

The first Small Expendable Deployer System (SEDS-1), a tethered satellite system, was developed by NASA and launched March 29, 1993 as a secondary payload on a United State Air Force (USAF) Delta-2 launch vehicle. The SEDS-1 successfully deployed an instrumented end-mass payload (EMP) on a 20-km nonconducting tether from the second stage of the Delta 2. This paper describes the effort of NASA Langley Research Center's Antenna and Microwave Research Branch to provide assistance to the SEDS Investigators Working Group (IWG) in determining EMP dynamics by analyzing the mission radar skin track data. The radar cross section measurements taken and simulations done for this study are described and comparisons of the measured data with the simulated data for the EMP at 6 GHz are presented.

A Two-Step Method to Select Major Surge-Producing Extratropical Cyclones from a 10,000-Year Stochastic Catalog

NASA Astrophysics Data System (ADS)

Keshtpoor, M.; Carnacina, I.; Yablonsky, R. M.

2016-12-01

Extratropical cyclones (ETCs) are the primary driver of storm surge events along the UK and northwest mainland Europe coastlines. In an effort to evaluate the storm surge risk in coastal communities in this region, a stochastic catalog is developed by perturbing the historical storm seeds of European ETCs to account for 10,000 years of possible ETCs. Numerical simulation of the storm surge generated by the full 10,000-year stochastic catalog, however, is computationally expensive and may take several months to complete with available computational resources. A new statistical regression model is developed to select the major surge-generating events from the stochastic ETC catalog. This regression model is based on the maximum storm surge, obtained via numerical simulations using a calibrated version of the Delft3D-FM hydrodynamic model with a relatively coarse mesh, of 1750 historical ETC events that occurred over the past 38 years in Europe. These numerically-simulated surge values were regressed to the local sea level pressure and the U and V components of the wind field at the location of 196 tide gauge stations near the UK and northwest mainland Europe coastal areas. The regression model suggests that storm surge values in the area of interest are highly correlated to the U- and V-component of wind speed, as well as the sea level pressure. Based on these correlations, the regression model was then used to select surge-generating storms from the 10,000-year stochastic catalog. Results suggest that roughly 105,000 events out of 480,000 stochastic storms are surge-generating events and need to be considered for numerical simulation using a hydrodynamic model. The selected stochastic storms were then simulated in Delft3D-FM, and the final refinement of the storm population was performed based on return period analysis of the 1750 historical event simulations at each of the 196 tide gauges in preparation for Delft3D-FM fine mesh simulations.

Comparison of equilibrium ohmic and nonequilibrium swarm models for monitoring conduction electron evolution in high-altitude EMP calculations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pusateri, Elise N.; Morris, Heidi E.; Nelson, Eric

2016-10-17

Here, atmospheric electromagnetic pulse (EMP) events are important physical phenomena that occur through both man-made and natural processes. Radiation-induced currents and voltages in EMP can couple with electrical systems, such as those found in satellites, and cause significant damage. Due to the disruptive nature of EMP, it is important to accurately predict EMP evolution and propagation with computational models. CHAP-LA (Compton High Altitude Pulse-Los Alamos) is a state-of-the-art EMP code that solves Maxwell inline images equations for gamma source-induced electromagnetic fields in the atmosphere. In EMP, low-energy, conduction electrons constitute a conduction current that limits the EMP by opposing themore » Compton current. CHAP-LA calculates the conduction current using an equilibrium ohmic model. The equilibrium model works well at low altitudes, where the electron energy equilibration time is short compared to the rise time or duration of the EMP. At high altitudes, the equilibration time increases beyond the EMP rise time and the predicted equilibrium ionization rate becomes very large. The ohmic model predicts an unphysically large production of conduction electrons which prematurely and abruptly shorts the EMP in the simulation code. An electron swarm model, which implicitly accounts for the time evolution of the conduction electron energy distribution, can be used to overcome the limitations exhibited by the equilibrium ohmic model. We have developed and validated an electron swarm model previously in Pusateri et al. (2015). Here we demonstrate EMP damping behavior caused by the ohmic model at high altitudes and show improvements on high-altitude, upward EMP modeling obtained by integrating a swarm model into CHAP-LA.« less

Comparison of equilibrium ohmic and nonequilibrium swarm models for monitoring conduction electron evolution in high-altitude EMP calculations

NASA Astrophysics Data System (ADS)

Pusateri, Elise N.; Morris, Heidi E.; Nelson, Eric; Ji, Wei

2016-10-01

Atmospheric electromagnetic pulse (EMP) events are important physical phenomena that occur through both man-made and natural processes. Radiation-induced currents and voltages in EMP can couple with electrical systems, such as those found in satellites, and cause significant damage. Due to the disruptive nature of EMP, it is important to accurately predict EMP evolution and propagation with computational models. CHAP-LA (Compton High Altitude Pulse-Los Alamos) is a state-of-the-art EMP code that solves Maxwell's equations for gamma source-induced electromagnetic fields in the atmosphere. In EMP, low-energy, conduction electrons constitute a conduction current that limits the EMP by opposing the Compton current. CHAP-LA calculates the conduction current using an equilibrium ohmic model. The equilibrium model works well at low altitudes, where the electron energy equilibration time is short compared to the rise time or duration of the EMP. At high altitudes, the equilibration time increases beyond the EMP rise time and the predicted equilibrium ionization rate becomes very large. The ohmic model predicts an unphysically large production of conduction electrons which prematurely and abruptly shorts the EMP in the simulation code. An electron swarm model, which implicitly accounts for the time evolution of the conduction electron energy distribution, can be used to overcome the limitations exhibited by the equilibrium ohmic model. We have developed and validated an electron swarm model previously in Pusateri et al. (2015). Here we demonstrate EMP damping behavior caused by the ohmic model at high altitudes and show improvements on high-altitude, upward EMP modeling obtained by integrating a swarm model into CHAP-LA.

Improving high-altitude emp modeling capabilities by using a non-equilibrium electron swarm model to monitor conduction electron evolution

NASA Astrophysics Data System (ADS)

Pusateri, Elise Noel

An Electromagnetic Pulse (EMP) can severely disrupt the use of electronic devices in its path causing a significant amount of infrastructural damage. EMP can also cause breakdown of the surrounding atmosphere during lightning discharges. This makes modeling EMP phenomenon an important research effort in many military and atmospheric physics applications. EMP events include high-energy Compton electrons or photoelectrons that ionize air and produce low energy conduction electrons. A sufficient number of conduction electrons will damp or alter the EMP through conduction current. Therefore, it is important to understand how conduction electrons interact with air in order to accurately predict the EMP evolution and propagation in the air. It is common for EMP simulation codes to use an equilibrium ohmic model for computing the conduction current. Equilibrium ohmic models assume the conduction electrons are always in equilibrium with the local instantaneous electric field, i.e. for a specific EMP electric field, the conduction electrons instantaneously reach steady state without a transient process. An equilibrium model will work well if the electrons have time to reach their equilibrium distribution with respect to the rise time or duration of the EMP. If the time to reach equilibrium is comparable or longer than the rise time or duration of the EMP then the equilibrium model would not accurately predict the conduction current necessary for the EMP simulation. This is because transport coefficients used in the conduction current calculation will be found based on equilibrium reactions rates which may differ significantly from their non-equilibrium values. We see this deficiency in Los Alamos National Laboratory's EMP code, CHAP-LA (Compton High Altitude Pulse-Los Alamos), when modeling certain EMP scenarios at high altitudes, such as upward EMP, where the ionization rate by secondary electrons is over predicted by the equilibrium model, causing the EMP to short abruptly. The objective of the PhD research is to mitigate this effect by integrating a conduction electron model into CHAP-LA which can calculate the conduction current based on a non-equilibrium electron distribution. We propose to use an electron swarm model to monitor the time evolution of conduction electrons in the EMP environment which is characterized by electric field and pressure. Swarm theory uses various collision frequencies and reaction rates to study how the electron distribution and the resultant transport coefficients change with time, ultimately reaching an equilibrium distribution. Validation of the swarm model we develop is a necessary step for completion of the thesis work. After validation, the swarm model is integrated in the air chemistry model CHAP-LA employs for conduction electron simulations. We test high altitude EMP simulations with the swarm model option in the air chemistry model to show improvements in the computational capability of CHAP-LA. A swarm model has been developed that is based on a previous swarm model developed by Higgins, Longmire and O'Dell 1973, hereinafter HLO. The code used for the swarm model calculation solves a system of coupled differential equations for electric field, electron temperature, electron number density, and drift velocity. Important swarm parameters, including the momentum transfer collision frequency, energy transfer collision frequency, and ionization rate, are recalculated and compared to the previously reported empirical results given by HLO. These swarm parameters are found using BOLSIG+, a two term Boltzmann solver developed by Hagelaar and Pitchford 2005. BOLSIG+ utilizes updated electron scattering cross sections that are defined over an expanded energy range found in the atomic and molecular cross section database published by Phelps in the Phelps Database 2014 on the LXcat website created by Pancheshnyi et al. 2012. The swarm model is also updated from the original HLO model by including additional physical parameters such as the O2 electron attachment rate, recombination rate, and mutual neutralization rate. This necessitates tracking the positive and negative ion densities in the swarm model. Adding these parameters, especially electron attachment, is important at lower EMP altitudes where atmospheric density is high. We compare swarm model equilibrium temperatures and times using the HLO and BOLSIG+ coefficients for a uniform electric field of 1 StatV/cm for a range of atmospheric heights. This is done in order to test sensitivity to the swarm parameters used in the swarm model. It is shown that the equilibrium temperature and time are sensitive to the modifications in the collision frequency and ionization rate based on the updated electron interaction cross sections. We validate the swarm model by comparing ionization coefficients and equilibrium drift velocities to experimental results over a wide range of reduced electric field values. The final part of the PhD thesis work includes integrating the swarm model into CHAP-LA. We discuss the physics included in the CHAP-LA EMP model and demonstrate EMP damping behavior caused by the ohmic model at high altitudes. We report on numerical techniques for incorporation of the swarm model into CHAP-LA's Maxwell solver. This includes a discussion of integration techniques for Maxwell's equations in CHAP-LA using the swarm model calculated conduction current. We show improvements on EMP parameter calculations when modeling a high altitude, upward EMP scenario. This provides a novel computational capability that will have an important impact on the atmospheric and EMP research community.

Simulation Analysis of DC and Switching Impulse Superposition Circuit

NASA Astrophysics Data System (ADS)

Zhang, Chenmeng; Xie, Shijun; Zhang, Yu; Mao, Yuxiang

2018-03-01

Surge capacitors running between the natural bus and the ground are affected by DC and impulse superposition voltage during operation in the converter station. This paper analyses the simulation aging circuit of surge capacitors by PSCAD electromagnetic transient simulation software. This paper also analyses the effect of the DC voltage to the waveform of the impulse voltage generation. The effect of coupling capacitor to the test voltage waveform is also studied. Testing results prove that the DC voltage has little effect on the waveform of the output of the surge voltage generator, and the value of the coupling capacitor has little effect on the voltage waveform of the sample. Simulation results show that surge capacitor DC and impulse superimposed aging test is feasible.

Modeling and simulation of storm surge on Staten Island to understand inundation mitigation strategies

USGS Publications Warehouse

Kress, Michael E.; Benimoff, Alan I.; Fritz, William J.; Thatcher, Cindy A.; Blanton, Brian O.; Dzedzits, Eugene

2016-01-01

Hurricane Sandy made landfall on October 29, 2012, near Brigantine, New Jersey, and had a transformative impact on Staten Island and the New York Metropolitan area. Of the 43 New York City fatalities, 23 occurred on Staten Island. The borough, with a population of approximately 500,000, experienced some of the most devastating impacts of the storm. Since Hurricane Sandy, protective dunes have been constructed on the southeast shore of Staten Island. ADCIRC+SWAN model simulations run on The City University of New York's Cray XE6M, housed at the College of Staten Island, using updated topographic data show that the coast of Staten Island is still susceptible to tidal surge similar to those generated by Hurricane Sandy. Sandy hindcast simulations of storm surges focusing on Staten Island are in good agreement with observed storm tide measurements. Model results calculated from fine-scaled and coarse-scaled computational grids demonstrate that finer grids better resolve small differences in the topography of critical hydraulic control structures, which affect storm surge inundation levels. The storm surge simulations, based on post-storm topography obtained from high-resolution lidar, provide much-needed information to understand Staten Island's changing vulnerability to storm surge inundation. The results of fine-scale storm surge simulations can be used to inform efforts to improve resiliency to future storms. For example, protective barriers contain planned gaps in the dunes to provide for beach access that may inadvertently increase the vulnerability of the area.

Storm Surge Modeling of Typhoon Haiyan at the Naval Oceanographic Office Using Delft3D

NASA Astrophysics Data System (ADS)

Gilligan, M. J.; Lovering, J. L.

2016-02-01

The Naval Oceanographic Office provides estimates of the rise in sea level along the coast due to storm surge associated with tropical cyclones, typhoons, and hurricanes. Storm surge modeling and prediction helps the US Navy by providing a threat assessment tool to help protect Navy assets and provide support for humanitarian assistance/disaster relief efforts. Recent advancements in our modeling capabilities include the use of the Delft3D modeling suite as part of a Naval Research Laboratory (NRL) developed Coastal Surge Inundation Prediction System (CSIPS). Model simulations were performed on Typhoon Haiyan, which made landfall in the Philippines in November 2013. Comparisons of model simulations using forecast and hindcast track data highlight the importance of accurate storm track information for storm surge predictions. Model runs using the forecast track prediction and hindcast track information give maximum storm surge elevations of 4 meters and 6.1 meters, respectively. Model results for the hindcast simulation were compared with data published by the JSCE-PICE Joint survey for locations in San Pedro Bay (SPB) and on the Eastern Samar Peninsula (ESP). In SPB, where wind-induced set-up predominates, the model run using the forecast track predicted surge within 2 meters in 38% of survey locations and within 3 meters in 59% of the locations. When the hindcast track was used, the model predicted within 2 meters in 77% of the locations and within 3 meters in 95% of the locations. The model was unable to predict the high surge reported along the ESP produced by infragravity wave-induced set-up, which is not simulated in the model. Additional modeling capabilities incorporating infragravity waves are required to predict storm surge accurately along open coasts with steep bathymetric slopes, such as those seen in island arcs.

Endothelial microparticles reduce ICAM-1 expression in a microRNA-222-dependent mechanism

PubMed Central

Jansen, Felix; Yang, Xiaoyan; Baumann, Katharina; Przybilla, David; Schmitz, Theresa; Flender, Anna; Paul, Kathrin; Alhusseiny, Adil; Nickenig, Georg; Werner, Nikos

2015-01-01

Endothelial microparticles (EMP) are released from activated or apoptotic endothelial cells (ECs) and can be taken up by adjacent ECs, but their effect on vascular inflammation after engulfment is largely unknown. We sought to determine the role of EMP in EC inflammation. In vitro, EMP treatment significantly reduced tumour necrosis factor-α-induced endothelial intercellular adhesion molecule (ICAM)-1 expression on mRNA and protein level, whereas there was no effect on vascular cell adhesion molecule-1 expression. Reduced ICAM-1 expression after EMP treatment resulted in diminished monocyte adhesion in vitro. In vivo, systemic treatment of ApoE−/− mice with EMP significantly reduced murine endothelial ICAM-1 expression. To explore the underlying mechanisms, Taqman microRNA array was performed and microRNA (miR)-222 was identified as the strongest regulated miR between EMP and ECs. Following experiments demonstrated that miR-222 was transported into recipient ECs by EMP and functionally regulated expression of its target protein ICAM-1 in vitro and in vivo. After simulating diabetic conditions, EMP derived from glucose-treated ECs contained significantly lower amounts of miR-222 and showed reduced anti-inflammatory capacity in vitro and in vivo. Finally, circulating miR-222 level was diminished in patients with coronary artery disease (CAD) compared to patients without CAD. EMPs promote anti-inflammatory effects in vitro and in vivo by reducing endothelial ICAM-1 expression via the transfer of functional miR-222 into recipient cells. In pathological hyperglycaemic conditions, EMP-mediated miR-222-dependent anti-inflammatory effects are reduced. PMID:26081516

Endothelial microparticles reduce ICAM-1 expression in a microRNA-222-dependent mechanism.

PubMed

Jansen, Felix; Yang, Xiaoyan; Baumann, Katharina; Przybilla, David; Schmitz, Theresa; Flender, Anna; Paul, Kathrin; Alhusseiny, Adil; Nickenig, Georg; Werner, Nikos

2015-09-01

Endothelial microparticles (EMP) are released from activated or apoptotic endothelial cells (ECs) and can be taken up by adjacent ECs, but their effect on vascular inflammation after engulfment is largely unknown. We sought to determine the role of EMP in EC inflammation. In vitro, EMP treatment significantly reduced tumour necrosis factor-α-induced endothelial intercellular adhesion molecule (ICAM)-1 expression on mRNA and protein level, whereas there was no effect on vascular cell adhesion molecule-1 expression. Reduced ICAM-1 expression after EMP treatment resulted in diminished monocyte adhesion in vitro. In vivo, systemic treatment of ApoE-/- mice with EMP significantly reduced murine endothelial ICAM-1 expression. To explore the underlying mechanisms, Taqman microRNA array was performed and microRNA (miR)-222 was identified as the strongest regulated miR between EMP and ECs. Following experiments demonstrated that miR-222 was transported into recipient ECs by EMP and functionally regulated expression of its target protein ICAM-1 in vitro and in vivo. After simulating diabetic conditions, EMP derived from glucose-treated ECs contained significantly lower amounts of miR-222 and showed reduced anti-inflammatory capacity in vitro and in vivo. Finally, circulating miR-222 level was diminished in patients with coronary artery disease (CAD) compared to patients without CAD. EMPs promote anti-inflammatory effects in vitro and in vivo by reducing endothelial ICAM-1 expression via the transfer of functional miR-222 into recipient cells. In pathological hyperglycaemic conditions, EMP-mediated miR-222-dependent anti-inflammatory effects are reduced. © 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

A Simple Graphical Method for Quantification of Disaster Management Surge Capacity Using Computer Simulation and Process-control Tools.

PubMed

Franc, Jeffrey Michael; Ingrassia, Pier Luigi; Verde, Manuela; Colombo, Davide; Della Corte, Francesco

2015-02-01

Surge capacity, or the ability to manage an extraordinary volume of patients, is fundamental for hospital management of mass-casualty incidents. However, quantification of surge capacity is difficult and no universal standard for its measurement has emerged, nor has a standardized statistical method been advocated. As mass-casualty incidents are rare, simulation may represent a viable alternative to measure surge capacity. Hypothesis/Problem The objective of the current study was to develop a statistical method for the quantification of surge capacity using a combination of computer simulation and simple process-control statistical tools. Length-of-stay (LOS) and patient volume (PV) were used as metrics. The use of this method was then demonstrated on a subsequent computer simulation of an emergency department (ED) response to a mass-casualty incident. In the derivation phase, 357 participants in five countries performed 62 computer simulations of an ED response to a mass-casualty incident. Benchmarks for ED response were derived from these simulations, including LOS and PV metrics for triage, bed assignment, physician assessment, and disposition. In the application phase, 13 students of the European Master in Disaster Medicine (EMDM) program completed the same simulation scenario, and the results were compared to the standards obtained in the derivation phase. Patient-volume metrics included number of patients to be triaged, assigned to rooms, assessed by a physician, and disposed. Length-of-stay metrics included median time to triage, room assignment, physician assessment, and disposition. Simple graphical methods were used to compare the application phase group to the derived benchmarks using process-control statistical tools. The group in the application phase failed to meet the indicated standard for LOS from admission to disposition decision. This study demonstrates how simulation software can be used to derive values for objective benchmarks of ED surge capacity using PV and LOS metrics. These objective metrics can then be applied to other simulation groups using simple graphical process-control tools to provide a numeric measure of surge capacity. Repeated use in simulations of actual EDs may represent a potential means of objectively quantifying disaster management surge capacity. It is hoped that the described statistical method, which is simple and reusable, will be useful for investigators in this field to apply to their own research.

EMP/GMD Phase 0 Report, A Review of EMP Hazard Environments and Impacts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rivera, Michael Kelly; Backhaus, Scott N.; Woodroffe, Jesse Richard

The purpose of this study is to determine methods to analyze the hazard environments, impacts, and consequences of different sources of electromagnetic pulse (EMP), including nuclear electromagnetic pulse (NEMP) and geomagnetic disturbance (GMD) on the U.S. electric power infrastructures and to use those methods to determine EMP and GMD events of concern. The study will be carried out in four phases, each of which will provide higher levels of analytic fidelity that focuses on those EMP/GMD sources and events that create significant consequences, or whose consequences are sufficiently uncertain, to require more in-depth study. This study will leverage the bestmore » experimental data; device, equipment and system models; and simulation tools currently available. This study focuses primarily on the bulk electric system (BES) including large generating stations, large power transformers, the transmission network, and transmission system protection. Electrical distribution systems may potentially be included, if warranted, after consideration of the consequences for the bulk power system.« less

Uncertainty and feasibility of dynamical downscaling for modeling tropical cyclones for storm surge simulation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Zhaoqing; Taraphdar, Sourav; Wang, Taiping

This paper presents a modeling study conducted to evaluate the uncertainty of a regional model in simulating hurricane wind and pressure fields, and the feasibility of driving coastal storm surge simulation using an ensemble of region model outputs produced by 18 combinations of three convection schemes and six microphysics parameterizations, using Hurricane Katrina as a test case. Simulated wind and pressure fields were compared to observed H*Wind data for Hurricane Katrina and simulated storm surge was compared to observed high-water marks on the northern coast of the Gulf of Mexico. The ensemble modeling analysis demonstrated that the regional model wasmore » able to reproduce the characteristics of Hurricane Katrina with reasonable accuracy and can be used to drive the coastal ocean model for simulating coastal storm surge. Results indicated that the regional model is sensitive to both convection and microphysics parameterizations that simulate moist processes closely linked to the tropical cyclone dynamics that influence hurricane development and intensification. The Zhang and McFarlane (ZM) convection scheme and the Lim and Hong (WDM6) microphysics parameterization are the most skillful in simulating Hurricane Katrina maximum wind speed and central pressure, among the three convection and the six microphysics parameterizations. Error statistics of simulated maximum water levels were calculated for a baseline simulation with H*Wind forcing and the 18 ensemble simulations driven by the regional model outputs. The storm surge model produced the overall best results in simulating the maximum water levels using wind and pressure fields generated with the ZM convection scheme and the WDM6 microphysics parameterization.« less

From cyclic ice streaming to Heinrich-like events: the grow-and-surge instability in the Parallel Ice Sheet Model

NASA Astrophysics Data System (ADS)

Feldmann, Johannes; Levermann, Anders

2017-08-01

Here we report on a cyclic, physical ice-discharge instability in the Parallel Ice Sheet Model, simulating the flow of a three-dimensional, inherently buttressed ice-sheet-shelf system which periodically surges on a millennial timescale. The thermomechanically coupled model on 1 km horizontal resolution includes an enthalpy-based formulation of the thermodynamics, a nonlinear stress-balance-based sliding law and a very simple subglacial hydrology. The simulated unforced surging is characterized by rapid ice streaming through a bed trough, resulting in abrupt discharge of ice across the grounding line which is eventually calved into the ocean. We visualize the central feedbacks that dominate the subsequent phases of ice buildup, surge and stabilization which emerge from the interaction between ice dynamics, thermodynamics and the subglacial till layer. Results from the variation of surface mass balance and basal roughness suggest that ice sheets of medium thickness may be more susceptible to surging than relatively thin or thick ones for which the surge feedback loop is damped. We also investigate the influence of different basal sliding laws (ranging from purely plastic to nonlinear to linear) on possible surging. The presented mechanisms underlying our simulations of self-maintained, periodic ice growth and destabilization may play a role in large-scale ice-sheet surging, such as the surging of the Laurentide Ice Sheet, which is associated with Heinrich events, and ice-stream shutdown and reactivation, such as observed in the Siple Coast region of West Antarctica.

A parabolic model of drag coefficient for storm surge simulation in the South China Sea

PubMed Central

Peng, Shiqiu; Li, Yineng

2015-01-01

Drag coefficient (Cd) is an essential metric in the calculation of momentum exchange over the air-sea interface and thus has large impacts on the simulation or forecast of the upper ocean state associated with sea surface winds such as storm surges. Generally, Cd is a function of wind speed. However, the exact relationship between Cd and wind speed is still in dispute, and the widely-used formula that is a linear function of wind speed in an ocean model could lead to large bias at high wind speed. Here we establish a parabolic model of Cd based on storm surge observations and simulation in the South China Sea (SCS) through a number of tropical cyclone cases. Simulation of storm surges for independent Tropical cyclones (TCs) cases indicates that the new parabolic model of Cd outperforms traditional linear models. PMID:26499262

A parabolic model of drag coefficient for storm surge simulation in the South China Sea.

PubMed

Peng, Shiqiu; Li, Yineng

2015-10-26

Drag coefficient (Cd) is an essential metric in the calculation of momentum exchange over the air-sea interface and thus has large impacts on the simulation or forecast of the upper ocean state associated with sea surface winds such as storm surges. Generally, Cd is a function of wind speed. However, the exact relationship between Cd and wind speed is still in dispute, and the widely-used formula that is a linear function of wind speed in an ocean model could lead to large bias at high wind speed. Here we establish a parabolic model of Cd based on storm surge observations and simulation in the South China Sea (SCS) through a number of tropical cyclone cases. Simulation of storm surges for independent Tropical cyclones (TCs) cases indicates that the new parabolic model of Cd outperforms traditional linear models.

A parabolic model of drag coefficient for storm surge simulation in the South China Sea

NASA Astrophysics Data System (ADS)

Peng, Shiqiu; Li, Yineng

2015-10-01

Drag coefficient (Cd) is an essential metric in the calculation of momentum exchange over the air-sea interface and thus has large impacts on the simulation or forecast of the upper ocean state associated with sea surface winds such as storm surges. Generally, Cd is a function of wind speed. However, the exact relationship between Cd and wind speed is still in dispute, and the widely-used formula that is a linear function of wind speed in an ocean model could lead to large bias at high wind speed. Here we establish a parabolic model of Cd based on storm surge observations and simulation in the South China Sea (SCS) through a number of tropical cyclone cases. Simulation of storm surges for independent Tropical cyclones (TCs) cases indicates that the new parabolic model of Cd outperforms traditional linear models.

Development of Operational Wave-Tide-Storm surges Coupling Prediction System

NASA Astrophysics Data System (ADS)

You, S. H.; Park, S. W.; Kim, J. S.; Kim, K. L.

2009-04-01

The Korean Peninsula is surrounded by the Yellow Sea, East China Sea, and East Sea. This complex oceanographic system includes large tides in the Yellow Sea and seasonally varying monsoon and typhoon events. For Korea's coastal regions, floods caused by wave and storm surges are among the most serious threats. To predict more accurate wave and storm surges, the development of coupling wave-tide-storm surges prediction system is essential. For the time being, wave and storm surges predictions are still made separately in KMA (Korea Meteorological Administration) and most operational institute. However, many researchers have emphasized the effects of tides and storm surges on wind waves and recommended further investigations into the effects of wave-tide-storm surges interactions and coupling module. In Korea, especially, tidal height and current give a great effect on the wave prediction in the Yellow sea where is very high tide and related research is not enough. At present, KMA has operated the wave (RWAM : Regional Wave Model) and storm surges/tide prediction system (STORM : Storm Surges/Tide Operational Model) for ocean forecasting. The RWAM is WAVEWATCH III which is a third generation wave model developed by Tolman (1989). The STORM is based on POM (Princeton Ocean Model, Blumberg and Mellor, 1987). The RWAM and STORM cover the northwestern Pacific Ocean from 115°E to 150°E and from 20°N to 52°N. The horizontal grid intervals are 1/12° in both latitudinal and longitudinal directions. These two operational models are coupled to simulate wave heights for typhoon case. The sea level and current simulated by storm surge model are used for the input of wave model with 3 hour interval. The coupling simulation between wave and storm surge model carried out for Typhoon Nabi (0514), Shanshan(0613) and Nari (0711) which were effected on Korea directly. We simulated significant wave height simulated by wave model and coupling model and compared difference between uncoupling and coupling cases for each typhoon. When the typhoon Nabi hit at southern coast of Kyushu, predicted significant wave height reached over 10 m. The difference of significant wave height between wave and wave-tide-storm surges model represents large variation at the southwestern coast of Korea with about 0.5 m. Other typhoon cases also show similar results with typhoon Nabi case. For typhoon Shanshan case the difference of significant wave height reached up to 0.3 m. When the typhoon Nari was affected in the southern coast of Korea, predicted significant wave height was about 5m. The typhoon Nari case also shows the difference of significant wave height similar with other typhoon cases. Using the observation from ocean buoy operated by KMA, we compared wave information simulated by wave and wave-storm surges coupling model. The significant wave height simulated by wave-tide-storm surges model shows the tidal modulation features in the western and southern coast of Korea. And the difference of significant wave height between two models reached up to 0.5 m. The coupling effect also can be identified in the wave direction, wave period and wave length. In addition, wave spectrum is also changeable due to coupling effect of wave-tide-storm surges model. The development, testing and application of a coupling module in which wave-tide-storm surges are incorporated within the frame of KMA Ocean prediction system, has been considered as a step forward in respect of ocean forecasting. In addition, advanced wave prediction model will be applicable to the effect of ocean in the weather forecasting system. The main purpose of this study is to show how the coupling module developed and to report on a series of experiments dealing with the sensitivities and real case prediction of coupling wave-tide-storm surges prediction system.

The simulation of Typhoon-induced coastal inundation in Busan, South Korea applying the downscaling technique

NASA Astrophysics Data System (ADS)

Jang, Dongmin; Park, Junghyun; Yuk, Jin-Hee; Joh, MinSu

2017-04-01

Due to typhoons, the south coastal cities including Busan in South Korea coastal are very vulnerable to a surge, wave and corresponding coastal inundation, and are affected every year. In 2016, South Korea suffered tremendous damage by typhoon 'Chaba', which was developed near east-north of Guam on Sep. 28 and had maximum 10-minute sustained wind speed of about 50 m/s, 1-minute sustained wind speed of 75 m/s and a minimum central pressure of 905 hpa. As 'Chaba', which is the strongest since typhoon 'Maemi' in 2003, hit South Korea on Oct. 5, it caused a massive economic and casualty damage to Ulsan, Gyeongju and Busan in South Korea. In particular, the damage of typhoon-induced coastal inundation in Busan, where many high-rise buildings and residential areas are concentrated near coast, was serious. The coastal inundation could be more affected by strong wind-induced wave than surge. In fact, it was observed that the surge height was about 1 m averagely and a significant wave height was about 8 m at coastal sea nearby Busan on Oct. 5 due to 'Chaba'. Even though the typhoon-induced surge elevated the sea level, the typhoon-induced long period wave with wave period of more than 15s could play more important role in the inundation. The present work simulated the coastal inundation induced by 'Chaba' in Busan, South Korea considering the effects of typhoon-induced surge and wave. For 'Chaba' hindcast, high resolution Weather Research and Forecasting model (WRF) was applied using a reanalysis data produced by NCEP (FNL 0.25 degree) on the boundary and initial conditions, and was validated by the observation of wind speed, direction and pressure. The typhoon-induced coastal inundation was simulated by an unstructured gird model, Finite Volume Community Ocean Model (FVCOM), which is fully current-wave coupled model. To simulate the wave-induced inundation, 1-way downscaling technique of multi domain was applied. Firstly, a mother's domain including Korean peninsula was simulated using wind and pressure produced by WRF to produce surge and wave. And then, the wave-induced inundation was simulated applying the surge height and wave height simulated by mother's model to the open boundary and initial condition of child's model which was ranged near Busan. Our simulated surge height is generally underestimated about 15 % due to the underestimation of surface pressure on WRF. However, since the effect of wave on inundation could be more significant than surge-induced forcing in this real system, our research could predict the typhoon-induced inundation by combining the surge and wave forcing in nested domain.

Development of Dimensionless Surge Response Functions for Hazard Assessment at Panama City, Florida

NASA Astrophysics Data System (ADS)

Taylor, N. R.; Irish, J. L.; Hagen, S. C.; Kaihatu, J. M.; McLaughlin, P. W.

2013-12-01

Reliable and robust methods of extreme value analysis in hurricane surge forecasting are of high importance in the coastal engineering profession. The Joint Probability Method (JPM) has become the preferred statistical method over the Historical Surge Population (HSP) method, due to its ability to give more accurate surge predictions, as demonstrated by Irish et. al in 2011 (J. Geophys. Res.). One disadvantage to this method is its high computational cost; a single location can require hundreds of simulated storms, each needing one thousand computational hours or more to complete. One way of overcoming this issue is to use an interpolating function, called a surge response function, to reduce the required number of simulations to a manageable number. These sampling methods, which use physical scaling laws, have been shown to significantly reduce the number of simulated storms needed for application of the JPM method. In 2008, Irish et. al. (J. Phys. Oceanogr.) demonstrated that hurricane surge scales primarily as a function of storm size and intensity. Additionally, Song et. al. in 2012 (Nat. Hazards) has shown that surge response functions incorporating bathymetric variations yield highly accurate surge estimates along the Texas coastline. This study applies the Song. et. al. model to 73 stations along the open coast, and 273 stations within the bays, in Panama City, Florida. The model performs well for the open coast and bay areas; surge levels at most stations along the open coast were predicted with RMS errors below 0.40 meters, and R2 values at or above 0.80. The R2 values for surge response functions within bays were consistently at or above 0.75. Surge levels at most stations within the North Bay and East Bay were predicted with RMS errors below 0.40 meters; within the West Bay, surge was predicted with RMS errors below 0.52 meters. Accurately interpolating surge values along the Panama City coast and bays enables efficient use of the JPM model in order to develop reliable probabilistic surge estimates for use in planning and design for hurricane mitigation.

Development of a global river-coastal coupling model and its application to flood simulation in Asian mega-delta regions

NASA Astrophysics Data System (ADS)

Ikeuchi, Hiroaki; Hirabayashi, Yukiko; Yamazaki, Dai; Muis, Sanne; Ward, Philip; Verlaan, Martin; Winsemius, Hessel; Kanae, Shinjiro

2017-04-01

The world's mega-delta regions and estuaries are susceptible to various water-related disasters, such as river flooding and storm surge. Moreover, simultaneous occurrence of them would be more devastating than a situation where they occur in isolation. Therefore, it is important to provide information about compound risks of fluvial and coastal floods at a large scale, both their statistical dependency as well as their combined resulting flooding in delta regions. Here we report on a first attempt to address this issue globally by developing a method to couple a global river model (CaMa-Flood) and a global tide and surge reanalysis (GTSR) dataset. A state-of-the-art global river routing model, CaMa-Flood, was modified to represent varying sea levels due to tides and storm surges as downstream boundary condition, and the GTSR dataset was post-processed to serve as inputs to the CaMa-Flood river routing simulation and a long-term simulation was performed to incorporate the temporal dependency between coastal tide and surge on the one hand, and discharge on the other. The coupled model was validated against observations, showing better simulation results of water levels in deltaic regions than simulation without GTSR. For example in the Ganges Delta, correlation coefficients were increased by 0.06, and root mean square errors were reduced by 0.22 m. Global coupling simulations revealed that storm surges affected river water levels in coastal regions worldwide, especially in low-lying flat areas with increases in water level larger than 0.5 m. By employing enhanced storm surge simulation with tropical storm tracks, we also applied the model to examine impacts of past hurricane and cyclone storm events on river flood inundation.

Endothelial microparticle-mediated transfer of MicroRNA-126 promotes vascular endothelial cell repair via SPRED1 and is abrogated in glucose-damaged endothelial microparticles.

PubMed

Jansen, Felix; Yang, Xiaoyan; Hoelscher, Marion; Cattelan, Arianna; Schmitz, Theresa; Proebsting, Sebastian; Wenzel, Daniela; Vosen, Sarah; Franklin, Bernardo S; Fleischmann, Bernd K; Nickenig, Georg; Werner, Nikos

2013-10-29

Repair of the endothelium after vascular injury is crucial for preserving endothelial integrity and preventing the development of vascular disease. The underlying mechanisms of endothelial cell repair are largely unknown. We sought to investigate whether endothelial microparticles (EMPs), released from apoptotic endothelial cells (ECs), influence EC repair. Systemic treatment of mice with EMPs after electric denudation of the endothelium accelerated reendothelialization in vivo. In vitro experiments revealed that EMP uptake in ECs promotes EC migration and proliferation, both critical steps in endothelial repair. To dissect the underlying mechanisms, Taqman microRNA array was performed, and microRNA (miR)-126 was identified as the predominantly expressed miR in EMPs. The following experiments demonstrated that miR-126 was transported into recipient human coronary artery endothelial cells by EMPs and functionally regulated the target protein sprouty-related, EVH1 domain-containing protein 1 (SPRED1). Knockdown of miR-126 in EMPs abrogated EMP-mediated effects on human coronary artery endothelial cell migration and proliferation in vitro and reendothelialization in vivo. Interestingly, after simulating diabetic conditions, EMPs derived from glucose-treated ECs contained significantly lower amounts of miR-126 and showed reduced endothelial repair capacity in vitro and in vivo. Finally, expression analysis of miR-126 in circulating microparticles from 176 patients with stable coronary artery disease with and without diabetes mellitus revealed a significantly reduced miR-126 expression in circulating microparticles from diabetic patients. Endothelial microparticles promote vascular endothelial repair by delivering functional miR-126 into recipient cells. In pathological hyperglycemic conditions, EMP-mediated miR-126-induced EC repair is altered.

A modeling study of coastal inundation induced by storm surge, sea-level rise, and subsidence in the Gulf of Mexico

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Zhaoqing; Wang, Taiping; Leung, Lai-Yung R.

The northern coasts of the Gulf of Mexico are highly vulnerable to the direct threats of climate change, such as hurricane-induced storm surge, and such risks can be potentially exacerbated by land subsidence and global sea level rise. This paper presents an application of a coastal storm surge model to study the coastal inundation process induced by tide and storm surge, and its response to the effects of land subsidence and sea level rise in the northern Gulf coast. An unstructured-grid Finite Volume Coastal Ocean Model was used to simulate tides and hurricane-induced storm surges in the Gulf of Mexico.more » Simulated distributions of co-amplitude and co-phase of semi-diurnal and diurnal tides are in good agreement with previous modeling studies. The storm surges induced by four historical hurricanes (Rita, Katrina, Ivan and Dolly) were simulated and compared to observed water levels at National Oceanic and Atmospheric Administration tide stations. Effects of coastal subsidence and future global sea level rise on coastal inundation in the Louisiana coast were evaluated using a parameter “change of inundation depth” through sensitivity simulations that were based on a projected future subsidence scenario and 1-m global sea level rise by the end of the century. Model results suggested that hurricane-induced storm surge height and coastal inundation could be exacerbated by future global sea level rise and subsidence, and that responses of storm surge and coastal inundation to the effects of sea level rise and subsidence are highly nonlinear and vary on temporal and spatial scales.« less

Engineering Techniques for Electromagnetic Pulse-Hardness Testing.

DTIC Science & Technology

electromagnetic pulse (EMP). The text describes energy sources, simulation techniques, test instrumentation, and testing techniques. Emphasis is on testing that can be accomplished by engineers with knowledge of electromagnetics and circuits. Complicated systems that require special expertise are described only to acquaint the reader with their characteristics. This text is intended to supplement the testing portion of DNA 2772T ’DNA EMP Awareness Course Notes.’

Development of Inundation Map for Bantayan Island, Cebu Using Delft3D-Flow Storm Surge Simulations of Typhoon Haiyan

NASA Astrophysics Data System (ADS)

Cuadra, Camille; Suarez, John Kenneth; Biton, Nophi Ian; Cabacaba, Krichi May; Lapidez, John Phillip; Santiago, Joy; Mahar Francisco Lagmay, Alfredo; Malano, Vicente

2014-05-01

On average, 20 typhoons enter the Philippine area of responsibility annually, making it vulnerable to different storm hazards. Apart from the frequency of tropical cyclones, the archipelagic nature of the country makes it particularly prone to storm surges. On 08 November 2013, Haiyan, a Category 5 Typhoon with maximum one-minute sustained wind speed of 315 kph, hit the central region of the Philippines. In its path, the howler devastated Bantayan Island, a popular tourist destination. The island is located north of Cebu City, the second largest metropolis of the Philippines in terms of populace. Having been directly hit by Typhoon Haiyan, Bantayan Island was severely damaged by strong winds and storm surges, with more than 11,000 houses totally destroyed while 5,000 more suffered minor damage. The adverse impacts of possible future storm surge events in the island can only be mitigated if hazard maps that depict inundation of the coastal areas of Bantayan are generated. To create such maps, Delft3D-Flow, a hydrodynamic model was used to simulate storm surges. These simulations were made over a 10-m per pixel resolution Digital Elevation Model (DEM) and the General Bathymetric Chart of the Oceans (GEBCO) bathymetry. The results of the coastal inundation model for Typhoon Haiyan's storm surges were validated using data collected from field work and local government reports. The hydrodynamic model of Bantayan was then calibrated using the field data and further simulations were made with varying typhoon tracks. This was done to generate scenarios on the farthest possible inland incursion of storm surges. The output of the study is a detailed storm surge inundation map that depicts safe zones for development of infrastructure near coastal areas and for construction of coastal protection structures. The storm surge inundation map can also be used as basis for disaster preparedness plans of coastal communities threatened by approaching typhoons.

Designsafe-Ci a Cyberinfrastructure for Natural Hazard Simulation and Data

NASA Astrophysics Data System (ADS)

Dawson, C.; Rathje, E.; Stanzione, D.; Padgett, J.; Pinelli, J. P.

2017-12-01

DesignSafe is the web-based research platform of the Natural Hazards Engineering Research Infrastructure (NHERI) network that provides the computational tools needed to manage and analyze critical data for natural hazards research, with wind and storm surge related hazards being a primary focus. One of the simulation tools under DesignSafe is the Advanced Circulation (ADCIRC) model, a coastal ocean model used in storm surge analysis. ADCIRC is an unstructured, finite element model with high resolution capabilities for studying storm surge impacts, and has long been used in storm surge hind-casting and forecasting. In this talk, we will demonstrate the use of ADCIRC within the DesignSafe platform and its use for forecasting Hurricane Harvey. We will also demonstrate how to analyze, visualize and archive critical storm surge related data within DesignSafe.

The effect of wave current interactions on the storm surge and inundation in Charleston Harbor during Hurricane Hugo 1989

NASA Astrophysics Data System (ADS)

Xie, Lian; Liu, Huiqing; Peng, Machuan

The effects of wave-current interactions on the storm surge and inundation induced by Hurricane Hugo in and around the Charleston Harbor and its adjacent coastal regions are examined by using a three-dimensional (3-D) wave-current coupled modeling system. The 3-D storm surge and inundation modeling component of the coupled system is based on the Princeton ocean model (POM), whereas the wave modeling component is based on the third-generation wave model, simulating waves nearshore (SWAN). The results indicate that the effects of wave-induced surface, bottom, and radiation stresses can separately or in combination produce significant changes in storm surge and inundation. The effects of waves vary spatially. In some areas, the contribution of waves to peak storm surge during Hurricane Hugo reached as high as 0.76 m which led to substantial changes in the inundation and drying areas simulated by the storm surge model.

Numerical experiments of dynamical processes during the 2011-2013 surge of the Bering-Bagley Glacier System, using a full-Stokes finite element model

NASA Astrophysics Data System (ADS)

Trantow, Thomas

The Bering-Bagley Glacial System (BBGS) is the largest glacier system outside of the Greenland and Antarctic ice sheets, and is the Earth's largest surge-type glacier. Surging is one of three types of glacial acceleration and the least understood one. Understanding glacial acceleration is paramount when trying to explain ice discharge to the oceans and the glacial contribution to sea-level rise, yet there are currently no numerical glacial models that account for surging. The recent 2011-2013 surge of the BBGS provides a rare opportunity to study the surge process through observations and the subsequent data analysis and numerical modeling. Using radar, altimeter, and image data collected from airborne and satellite missions, various descriptions of ice geometry are created at different times throughout the surge. Using geostatistical estimation techniques including variography and ordinary kriging, surface and bedrock Digital Elevation Maps (DEMs) are derived. A time series analysis of elevation change during the current surge is then conducted and validated using a complete error analysis along with airborne observations. The derived DEMs are then used as inputs to a computer simulated model of glacier dynamics in the BBGS. Using the Finite Element software Elmer/Ice, a full-Stokes simulation, with Glen's flow law for temperate ice, is created for numerical experiments. With consideration of free surface evolution, glacial hydrology and surface mass balance, the model is able to predict a variety of field variables including velocity, stress, strain-rate, pressure and surface elevation change at any point forward in time. These outputs are compared and validated using observational data such as CryoSat-2 altimetry, airborne field data, imagery and previous detailed analysis of the BBGS. Preliminary results reveal that certain surge phenomena such as surface elevation changes, surge progression and locations at which the surge starts, can be recreated using the current model. Documentation of the effects that altering glaciological parameters and boundary conditions have on ice rheology in a large complex glacial system comes as secondary result. Simulations have yet to reveal any quasi-cyclic behavior or natural surge initiation.

Storm Surge Simulation and Ensemble Forecast for Hurricane Irene (2011)

NASA Astrophysics Data System (ADS)

Lin, N.; Emanuel, K.

2012-12-01

Hurricane Irene, raking the U.S. East Coast during the period of 26-30 August 2011, caused widespread damage estimated at $15.8 billion and was responsible for 49 direct deaths (Avila and Cangialosi, 2011). Although the most severe impact in the northeastern U.S. was catastrophic inland flooding, with its unusually large size, Irene also generated high waves and storm surges and caused moderate to major coastal flooding. The most severe surge damage occurred between Oregon Inlet and Cape Hatteras in North Carolina (NC). Significant storm surge damage also occurred along southern Chesapeake Bay, and moderate and high surges were observed along the coast from New Jersey (NJ) northward. A storm surge of 0.9-1.8 m caused hundreds of millions of dollars in property damage in New York City (NYC) and Long Island, despite the fact that the storm made landfall to the west of NYC with peak winds of no more than tropical storm strength. Making three U.S. landfalls (in NC, NJ, and NY), Hurricane Irene provides a unique case for studying storm surge along the eastern U.S. coastline. We apply the hydrodynamic model ADCIRC (Luettich et al. 1992) to conduct surge simulations for Pamlico Sound, Chesapeake Bay, and NYC, using best track data and parametric wind and pressure models. The results agree well with tidal-gauge observations. Then we explore a new methodology for storm surge ensemble forecasting and apply it to Irene. This method applies a statistical/deterministic hurricane model (Emanuel et al. 2006) to generate large numbers of storm ensembles under the storm environment described by the 51 ECMWF ensemble members. The associated surge ensembles are then generated with the ADCIRC model. The numerical simulation is computationally efficient, making the method applicable to real-time storm surge ensemble forecasting. We report the results for NYC in this presentation. The ADCIRC simulation using the best track data generates a storm surge of 1.3 m and a storm tide of 2.1 m at the Battery, NYC, which agree well with the observed storm surge of 1.33 m and storm tide of 2.12 m, although the simulated surge arrives about 2 hours earlier than the observed. Based on the surge climatology estimated by Lin et al. (2012), Hurricane Irene's storm surge is approximately a 60-year event for NYC, but its storm tide, with the surge happening right at the high astronomical tide, is a 100-year event. Lin et al. (2012) also projected that such 100-year storm tide events might occur on average every 3-20 years by the end of the century, under the IPCC A1B emission scenario and a 1-m sea level rise. The ensemble forecasting, starting from two and one days (each with 1000 ensembles) before Irene's first landfall in NC, shows that Irene's actual storm surge at the Battery had a chance of about 9% and 10% to be exceeded, respectively. The largest surges among the two ensemble sets are 2.28 m and 2.05 m, respectively. If happening at the high tide, as with Hurricane Irene, the worst-case storm tides would be about 3-3.2 m, similar to the highest historical water level at the Battery due to a hurricane in 1821. Lin et al. (2012) estimated that such a storm tide of about 3.1 m had a return period of about 500 years under current climate conditions, but the return period might become 25-240 years by the end of the century, under the IPCC A1B emission scenario and a 1-m sea level rise.

XM-1 Tank EMP Susceptibility and Survivability Test Program and Plan

DTIC Science & Technology

1980-11-01

electric field vector. The Vertical EMP Electromagnetic interference (EMI) shielding Simulator ( VEMPS ) produces a non-threat- is used on cable...polarized fields in the VEMPS to determine 2.3 Oveiall Program Activity Flow 5 , bulk current waveforms on interior cabling Figure 1 (p. 8) expresses...measured. The vertically polarized VEMPS the ground, it is not readily obvious how the will be used to measure harness sheath cur- currents on the

The Response of Extreme Precipitation to Climate Change in the North American Monsoon Region

NASA Astrophysics Data System (ADS)

Pascale, S.; Bordoni, S.; Kapnick, S. B.; Delworth, T. L.; Murakami, H.

2017-12-01

Gulf of California moisture surges (GoC surges) transport lower-level moisture in the southwestern United States and can trigger widespread convective bursts during the summertime North American monsoon (NAM). The intensity of such bursts varies over a wide spectrum, going from drier-than-average to extremely intense and persisting events. In this study we use a 50 km-horizontal resolution global coupled model (FLOR) developed at the NOAA Geophysical Fluid Dynamics Laboratory and featuring a realistic simulation of the GoC surges. We evaluate the model's ability to reproduce the intensity of precipitation during GoC surge and non-surge periods in present and doubled CO2 climatic conditions. We find that the mean number of GoC surge events per monsoon season (i.e., approximately 15) is not significantly affected by CO2 forcing. Nevertheless, when SST biases are minimized through flux adjustment, FLOR predicts a reduction in monsoonal precipitation over the southwestern United States. Our simulations further suggest that surge-related rainfall adjusts towards lower and higher percentiles, while becoming less important at intermediate values. Convective precipitation not occurring during GoC surges is instead not coherently affected by doubled CO2. Finally, the influence of CO2 forcing on the large-scale drivers of monsoonal precipitation during GoC surge events, such as the position of the monsoonal ridge, is investigated and related to precipitation changes.

Effect of Tide Elevation on Extratropical Storm Surge in Northwest Europe

NASA Astrophysics Data System (ADS)

Keshtpoor, M.; Carnacina, I.; Yablonsky, R. M.

2016-12-01

Extratropical cyclones (ETCs) are the major storm surge-generating meteorological events in northwest Europe. The total water level increase induced by these ETCs is significantly influenced by the local tidal range, which exceeds 8 meters along the southwestern UK coastline. In particular, a surge-generating ETC during high tide may put coastal assets and infrastructure in risk. Also, during low tide, the risk of surge induced by extreme ETC events is diminished. Here, the effect of tidal elevation on storm surge is investigated at 196 tide gauges in northwest Europe. A numerical, hydrodynamic model was developed using Delft3D-FM framework to simulate the coastal hydrodynamics during ETCs. Then, 1750 historical events were simulated to investigate the pattern of coastal inundation. Results suggest that in areas with a large tidal range ( 8 meters) and during the time period surrounding high or low tide, the pattern of coastal hydrodynamics is governed by tide and not storm surge. This result is most evident near the English Channel and Bristol Channel, where low frequency maximum water levels are observed when storm surge is combined with high tide. In contrast, near the tidal phase reversal, coastal hydrodynamics responds primarily to the storm surge, and low frequency maximum water elevation largely depends on the surge. In the areas with a small tidal range, ETC strength determines the pattern of coastal inundation.

Using wind setdown and storm surge on Lake Erie to calibrate the air-sea drag coefficient.

PubMed

Drews, Carl

2013-01-01

The air-sea drag coefficient controls the transfer of momentum from wind to water. In modeling storm surge, this coefficient is a crucial parameter for estimating the surge height. This study uses two strong wind events on Lake Erie to calibrate the drag coefficient using the Coupled Ocean Atmosphere Wave Sediment Transport (COAWST) modeling system and the the Regional Ocean Modeling System (ROMS). Simulated waves are generated on the lake with Simulating WAves Nearshore (SWAN). Wind setdown provides the opportunity to eliminate wave setup as a contributing factor, since waves are minimal at the upwind shore. The study finds that model results significantly underestimate wind setdown and storm surge when a typical open-ocean formulation without waves is used for the drag coefficient. The contribution of waves to wind setdown and storm surge is 34.7%. Scattered lake ice also increases the effective drag coefficient by a factor of 1.1.

Comparison of Coastal Inundation in the Outer Banks during Three Recent Hurricanes

NASA Astrophysics Data System (ADS)

Liu, T.; Sheng, Y.

2012-12-01

Coastal inundation in the Outer Banks and Chesapeake Bay during several recent hurricanes - Isabel, Earl and Irene, in 2005, 2010 and 2011, respectively, have been successfully simulated using the storm surge modeling system, CH3D-SSMS, which includes coupled coastal and basin-scale storm surge and wave models. Hurricane Isabel, which made landfall at the Outer Banks area in 2005, generated high waves up to 20 m offshore and 2.5 m inside the Chesapeake Bay which significantly affected the peak surge, with wave induced set-up contributing up to about 20% of the peak surge. During Isabel, the observed wave height at Duck station (1 km offshore) reached over 6 meters at landfall time, while Earl and Irene generated relatively moderate waves, with peak wave height around 4 meters at that station but a much lower wave height before landfall. Simulations show that during Earl and Irene, wave induced set-up did not contribute as much as that during Isabel. At Duck Pier, wave effects accounted for ~36 cm or 20% of the peak surge of 1.71 m during Isabel, while waves contributed ~10 cm (10%) toward the peak surge of 1 m during Irene and even less during Earl. The maximum surge during Irene was largely caused by the strong wind, as confirmed by the model using H* wind. Inundation maps have been generated and compared based on the simulations of Isabel, Earl and Irene.

Numerical simulation of MEMS-based blade load distribution control in centrifugal compressor surge suppression

NASA Astrophysics Data System (ADS)

Beneda, Károly

2012-11-01

The utilization of turbomachines requires up-to-date technologies to ensure safe operation throughout the widest possible range that makes novel ideas necessary to cope with classic problems. One of the most dangerous instability in compression systems is surge that has to be suppressed before its onset to avoid structural damages as well as other adverse consequences in the system. As surge occurs at low delivered mass flow rates the conventional widely spread surge control is based on bypassing the unnecessary airflow back to the atmosphere. This method has been implemented on a large number of aircraft and provides a robust control on suppressing compressor surge while creating a significant efficiency loss. This paper deals with an idea that has been originally designed as a fixed geometry that could be realized using up-to-date MEMS technology resulting in moderate losses but comparable stability enhancement. Previously the author has established the one-dimensional mathematical model of the concept, but it is indispensable - before the real instrument can be developed - to carry out detailed numerical simulation of the device. The aim of the paper is to acquaint the efforts of this CFD simulation.

The Development of High-speed Full-function Storm Surge Model and the Case Study of 2013 Typhoon Haiyan

NASA Astrophysics Data System (ADS)

Tsai, Y. L.; Wu, T. R.; Lin, C. Y.; Chuang, M. H.; Lin, C. W.

2016-02-01

An ideal storm surge operational model should feature as: 1. Large computational domain which covers the complete typhoon life cycle. 2. Supporting both parametric and atmospheric models. 3. Capable of calculating inundation area for risk assessment. 4. Tides are included for accurate inundation simulation. Literature review shows that not many operational models reach the goals for the fast calculation, and most of the models have limited functions. In this paper, a well-developed COMCOT (COrnell Multi-grid Coupled of Tsunami Model) tsunami model is chosen as the kernel to establish a storm surge model which solves the nonlinear shallow water equations on both spherical and Cartesian coordinates directly. The complete evolution of storm surge including large-scale propagation and small-scale offshore run-up can be simulated by nested-grid scheme. The global tide model TPXO 7.2 established by Oregon State University is coupled to provide astronomical boundary conditions. The atmospheric model named WRF (Weather Research and Forecasting Model) is also coupled to provide metrological fields. The high-efficiency thin-film method is adopted to evaluate the storm surge inundation. Our in-house model has been optimized by OpenMp (Open Multi-Processing) with the performance which is 10 times faster than the original version and makes it an early-warning storm surge model. In this study, the thorough simulation of 2013 Typhoon Haiyan is performed. The detailed results will be presented in Oceanic Science Meeting of 2016 in terms of surge propagation and high-resolution inundation areas.

Simulation of the westward traveling surge and Pi 2 pulsations during substorms

NASA Technical Reports Server (NTRS)

Kan, J. R.; Sun, W.

1985-01-01

The westward traveling surge and the Pi2 pulsations are simulated as a consequence of an enhanced magnetospheric convection in a model of magnetosphere coupling. The coupling is characterized by the bouncing of Alfven waves launched by the enhanced convection. The reflection of Alfven waves from the ionosphere is treated in which the height-integrated conductivity is allowed to be highly nonuniform and fully anisotropic. The reflection of Alfven waves from the magnetosphere is characterized by the coefficient Rm, depending on whether the field lines are open or closed. The conductivity in the model is self-consistently enhanced with increasing upward field-aligned current density. The results of the simulation, including the convection pattern, the electrojets, the field-aligned current, the conductivity enhancement, the oscillation of the westward electrojet, and the average speed of the westward surge are in reasonable agreement with the features of the westward traveling surge and the Pi 2 pulsations observed during substorms.

Data acquisition, processing and firing aid software for multichannel EMP simulation

NASA Astrophysics Data System (ADS)

Eumurian, Gregoire; Arbaud, Bruno

1986-08-01

Electromagnetic compatibility testing yields a large quantity of data for systematic analysis. An automated data acquisition system has been developed. It is based on standard EMP instrumentation which allows a pre-established program to be followed whilst orientating the measurements according to the results obtained. The system is controlled by a computer running interactive programs (multitask windows, scrollable menus, mouse, etc.) which handle the measurement channels, files, displays and process data in addition to providing an aid to firing.

Improved water-level forecasting for the Northwest European Shelf and North Sea through direct modelling of tide, surge and non-linear interaction

NASA Astrophysics Data System (ADS)

Zijl, Firmijn; Verlaan, Martin; Gerritsen, Herman

2013-07-01

In real-time operational coastal forecasting systems for the northwest European shelf, the representation accuracy of tide-surge models commonly suffers from insufficiently accurate tidal representation, especially in shallow near-shore areas with complex bathymetry and geometry. Therefore, in conventional operational systems, the surge component from numerical model simulations is used, while the harmonically predicted tide, accurately known from harmonic analysis of tide gauge measurements, is added to forecast the full water-level signal at tide gauge locations. Although there are errors associated with this so-called astronomical correction (e.g. because of the assumption of linearity of tide and surge), for current operational models, astronomical correction has nevertheless been shown to increase the representation accuracy of the full water-level signal. The simulated modulation of the surge through non-linear tide-surge interaction is affected by the poor representation of the tide signal in the tide-surge model, which astronomical correction does not improve. Furthermore, astronomical correction can only be applied to locations where the astronomic tide is known through a harmonic analysis of in situ measurements at tide gauge stations. This provides a strong motivation to improve both tide and surge representation of numerical models used in forecasting. In the present paper, we propose a new generation tide-surge model for the northwest European Shelf (DCSMv6). This is the first application on this scale in which the tidal representation is such that astronomical correction no longer improves the accuracy of the total water-level representation and where, consequently, the straightforward direct model forecasting of total water levels is better. The methodology applied to improve both tide and surge representation of the model is discussed, with emphasis on the use of satellite altimeter data and data assimilation techniques for reducing parameter uncertainty. Historic DCSMv6 model simulations are compared against shelf wide observations for a full calendar year. For a selection of stations, these results are compared to those with astronomical correction, which confirms that the tide representation in coastal regions has sufficient accuracy, and that forecasting total water levels directly yields superior results.

Risk assessment of storm surge disaster based on numerical models and remote sensing

NASA Astrophysics Data System (ADS)

Liu, Qingrong; Ruan, Chengqing; Zhong, Shan; Li, Jian; Yin, Zhonghui; Lian, Xihu

2018-06-01

Storm surge is one of the most serious ocean disasters in the world. Risk assessment of storm surge disaster for coastal areas has important implications for planning economic development and reducing disaster losses. Based on risk assessment theory, this paper uses coastal hydrological observations, a numerical storm surge model and multi-source remote sensing data, proposes methods for valuing hazard and vulnerability for storm surge and builds a storm surge risk assessment model. Storm surges in different recurrence periods are simulated in numerical models and the flooding areas and depth are calculated, which are used for assessing the hazard of storm surge; remote sensing data and GIS technology are used for extraction of coastal key objects and classification of coastal land use are identified, which is used for vulnerability assessment of storm surge disaster. The storm surge risk assessment model is applied for a typical coastal city, and the result shows the reliability and validity of the risk assessment model. The building and application of storm surge risk assessment model provides some basis reference for the city development plan and strengthens disaster prevention and mitigation.

Simulating storm surge inundation and damage potential within complex port facilities

NASA Astrophysics Data System (ADS)

Mawdsley, Robert; French, Jon; Fujiyama, Taku; Achutan, Kamalasudhan

2017-04-01

Storm surge inundation of port facilities can cause damage to critical elements of infrastructure, significantly disrupt port operations and cause downstream impacts on vital supply chains. A tidal surge in December 2013 in the North Sea partly flooded the Port of Immingham, which handles the largest volume of bulk cargo in the UK including major flows of coal and biomass for power generation. This flooding caused damage to port and rail transport infrastructure and disrupted operations for several weeks. This research aims to improve resilience to storm surges using hydrodynamic modelling coupled to an agent-based model of port operations. Using the December 2013 event to validate flood extent, depth and duration, we ran a high resolution hydrodynamic simulation using the open source Telemac 2D finite element code. The underlying Digital Elevation Model (DEM) was derived from Environment Agency LiDAR data, with ground truthing of the flood defences along the port frontage. Major infrastructure and buildings are explicitly resolved with varying degrees of permeability. Telemac2D simulations are run in parallel and take only minutes on a single 16 cpu compute node. Inundation characteristics predicted using Telemac 2D differ from a simple Geographical Information System 'bath-tub' analysis of the DEM based upon horizontal application of the maximum water level across the port topography. The hydrodynamic simulation predicts less extensive flooding and more closely matches observed flood extent. It also provides more precise depth and duration curves. Detailed spatial flood depth and duration maps were generated for a range of tide and surge scenarios coupled to mean sea-level rise projections. These inundation scenarios can then be integrated with critical asset databases and an agent-based model of port operation (MARS) that is capable of simulating storm surge disruption along wider supply chains. Port operators are able to act on information from a particular flood scenario to perform adaptive responses (e.g. pre-emptive relocation of equipment), as well as estimate the likely duration of any disruption to port and supply chain operation. High resolution numerical inundation modelling, coupled to accurate storm surge forecasting and an agent based port operation model, thus has the potential to significantly reduce damage and disruption costs associated with storm surge impacts on port infrastructure and systems.

Using Wind Setdown and Storm Surge on Lake Erie to Calibrate the Air-Sea Drag Coefficient

PubMed Central

Drews, Carl

2013-01-01

The air-sea drag coefficient controls the transfer of momentum from wind to water. In modeling storm surge, this coefficient is a crucial parameter for estimating the surge height. This study uses two strong wind events on Lake Erie to calibrate the drag coefficient using the Coupled Ocean Atmosphere Wave Sediment Transport (COAWST) modeling system and the the Regional Ocean Modeling System (ROMS). Simulated waves are generated on the lake with Simulating WAves Nearshore (SWAN). Wind setdown provides the opportunity to eliminate wave setup as a contributing factor, since waves are minimal at the upwind shore. The study finds that model results significantly underestimate wind setdown and storm surge when a typical open-ocean formulation without waves is used for the drag coefficient. The contribution of waves to wind setdown and storm surge is 34.7%. Scattered lake ice also increases the effective drag coefficient by a factor of 1.1. PMID:23977309

Nuclear Electromagnetic Pulse Review

NASA Astrophysics Data System (ADS)

Dinallo, Michael

2011-04-01

Electromagnetic Pulse (EMP) from nuclear detonations have been observed for well over half a century. Beginning in the mid-to-late 1950s, the physics and modeling of EMP has been researched and will continue into the foreseeable future. The EMP environment propagates hundreds of miles from its origins and causes interference for all types of electronic instrumentation. This includes military, municipal and industry based electronic infrastructures such as power generation and distribution, command and control systems, systems used in financial and emergency services, electronic monitoring and communications networks, to mention some key infrastructure elements. Research into EMP has included originating physics, propagation and electromagnetic field coupling analyses and measurement-sensor development. Several methods for calculating EMP induced transient interference (voltage and current induction) will be briefly discussed and protection techniques reviewed. These methods can be mathematically simple or involve challenging boundary value solution techniques. A few illustrative calculations will demonstrate the concern for electronic system operability. Analyses such as the Wunsch-Bell model for electronic upset or damage, and the Singularity Expansion Method (SEM) put forth by Dr. Carl Baum, will facilitate the concern for EMP effects. The SEM determines the voltages and currents induced from transient electromagnetic fields in terms of natural modes of various types of electronic platforms (aerospace vehicles or land-based assets - fixed or mobile). Full-scale facility and laboratory simulation and response measurement approaches will be discussed. The talk will conclude with a discussion of some present research activities.

SPH Simulation of Impact of a Surge on a Wall

NASA Astrophysics Data System (ADS)

Diwakar, Manoj Kumar; Mohapatra, Pranab Kumar; Tripathi, Shivam

2014-05-01

Structures located on the downstream of a dam are prone to impact of the surge due to dam break flow. Ramsden (1996) experimentally studied the run-up height on a vertical wall due to propagation of bore and surge on dry bed and measured their impact on the wall. Mohapatra et al. (2000) applied Navier Stokes equations to numerically study the impact of bore on vertical and inclined walls. They also obtained the evolution of surge on dry bed. In the present work, the impact of a surge wave due to dam break flow against the wall is modeled with a two-dimensional smoothed particle hydrodynamics (SPH) model. SPH is a mesh-free method that relies on the particle view of the field problem and approximates the continuity and momentum equations on a set of particles. The method solves the strong form of Navier-Stokes equations. The governing equations are solved numerically in the vertical plane. The propagation of the surge wave, its impact and the maximum run-up on the wall located at the boundary are analyzed. Surface profile, velocity field and pressure distributions are simulated. Non-dimensional run-up height obtained from the present numerical model is 0.86 and is in good agreement with the available experimental data of Ramsden (1996) which is in the range of 0.75-0.9. Also, the simulated profile of the surge tip was comparable to the empirical equations refereed in Ramsden (1996). The model is applied to the study the maximum force and the run-up height on inclined walls with different inclinations. The results indicate that the maximum force and the run-up height on the wall increase with the increment of wall inclination. Comparison of numerical results with analytical solutions derived from shallow water equations clearly shows the breakdown of shallow water assumption during the impact. In addition to these results, the numerical simulation yields the complete velocity and pressure ?elds which may be used to design structures located in the path of a dam-break wave. The study shows that the smoothed particle hydrodynamics can effectively simulate fluid flow dynamics. References: Mohapatra, P. K., Bhallamudi, S. M., and Eswaran, V. (2000). 'Numerical simulation of impact of bores against inclined walls.' J. Hydraulic. Engg., ASCE, 126(12), 942-945. Ramsden, J. D. (1996). 'Forces on a vertical wall due to long waves, bores, and dry-bed surges.' J. Waterway, Port, Coastal, and Ocean Engg., ASCE, 122(3), 134-141.

A stochastic storm surge generator for the German North Sea and the multivariate statistical assessment of the simulation results

NASA Astrophysics Data System (ADS)

Wahl, Thomas; Jensen, Jürgen; Mudersbach, Christoph

2010-05-01

Storm surges along the German North Sea coastline led to major damages in the past and the risk of inundation is expected to increase in the course of an ongoing climate change. The knowledge of the characteristics of possible storm surges is essential for the performance of integrated risk analyses, e.g. based on the source-pathway-receptor concept. The latter includes the storm surge simulation/analyses (source), modelling of dike/dune breach scenarios (pathway) and the quantification of potential losses (receptor). In subproject 1b of the German joint research project XtremRisK (www.xtremrisk.de), a stochastic storm surge generator for the south-eastern North Sea area is developed. The input data for the multivariate model are high resolution sea level observations from tide gauges during extreme events. Based on 25 parameters (19 sea level parameters and 6 time parameters) observed storm surge hydrographs consisting of three tides are parameterised. Followed by the adaption of common parametric probability distributions and a large number of Monte-Carlo-Simulations, the final reconstruction leads to a set of 100.000 (default) synthetic storm surge events with a one-minute resolution. Such a data set can potentially serve as the basis for a large number of applications. For risk analyses, storm surges with peak water levels exceeding the design water levels are of special interest. The occurrence probabilities of the simulated extreme events are estimated based on multivariate statistics, considering the parameters "peak water level" and "fullness/intensity". In the past, most studies considered only the peak water levels during extreme events, which might not be the most important parameter in any cases. Here, a 2D-Archimedian copula model is used for the estimation of the joint probabilities of the selected parameters, accounting for the structures of dependence overlooking the margins. In coordination with subproject 1a, the results will be used as the input for the XtremRisK subprojects 2 to 4. The project is funded by the German Federal Ministry of Education and Research (BMBF) (Project No. 03 F 0483 B).

Storm Surge and Tide Interaction: A Complete Paradigm

NASA Astrophysics Data System (ADS)

Horsburgh, K.

2014-12-01

Estimates show that in 2005, in the largest 136 coastal cities, there were 40 million people and 3,000 billion of assets exposed to 1 in 100 year coastal flood events. Mean sea level rise will increase this exposure to 150 million people and 35,000 billion of assets by 2070. Any further change in the statistics of flood frequency or severity would impact severely on economic and social systems. It is therefore crucial to understand the physical drivers of extreme storm surges, and to have confidence in datasets used for extreme sea level statistics. Much previous research has focussed on the process of tide-surge interaction, and it is now widely accepted that the physical basis of tide-surge interaction is that a phase shift of the tidal signal represents the effect of the surge on the tide. The second aspect of interaction is that shallow water momentum considerations imply that differing tidal states should modulate surge generation: wind stress should have greater surge-generating potential on lower tides. We present results from a storm surge model of the European shelf that demonstrate that tidal range does have an effect on the surges generated. The cycle-integrated effects of wind stress (i.e. the skew surge) are greater when tidal range is low. Our results contradict the absence of any such correlation in tide gauge records. This suggests that whilst the modulating effect of the tide on the skew surge (the time-independent difference between peak prediction and observations) is significant, the difference between individual storms is dominant. This implies that forecasting systems must predict salient detail of the most intense storms. A further implication is that flood forecasting models need to simulate tides with acceptable accuracy at all coastal locations. We extend our model analysis to show that the same modulation of storm surges (by tidal conditions) applies to tropical cyclones. We conduct simulations using a mature operational storm surge model in the Bay of Bengal with tropical cyclones from the IBTrACs database; we demonstrate that - just as with the extra-tropical case - higher storm surges on the Bangladesh coastline are generated during smaller tides.

Using Adaptive Mesh Refinment to Simulate Storm Surge

NASA Astrophysics Data System (ADS)

Mandli, K. T.; Dawson, C.

2012-12-01

Coastal hazards related to strong storms such as hurricanes and typhoons are one of the most frequently recurring and wide spread hazards to coastal communities. Storm surges are among the most devastating effects of these storms, and their prediction and mitigation through numerical simulations is of great interest to coastal communities that need to plan for the subsequent rise in sea level during these storms. Unfortunately these simulations require a large amount of resolution in regions of interest to capture relevant effects resulting in a computational cost that may be intractable. This problem is exacerbated in situations where a large number of similar runs is needed such as in design of infrastructure or forecasting with ensembles of probable storms. One solution to address the problem of computational cost is to employ adaptive mesh refinement (AMR) algorithms. AMR functions by decomposing the computational domain into regions which may vary in resolution as time proceeds. Decomposing the domain as the flow evolves makes this class of methods effective at ensuring that computational effort is spent only where it is needed. AMR also allows for placement of computational resolution independent of user interaction and expectation of the dynamics of the flow as well as particular regions of interest such as harbors. The simulation of many different applications have only been made possible by using AMR-type algorithms, which have allowed otherwise impractical simulations to be performed for much less computational expense. Our work involves studying how storm surge simulations can be improved with AMR algorithms. We have implemented relevant storm surge physics in the GeoClaw package and tested how Hurricane Ike's surge into Galveston Bay and up the Houston Ship Channel compares to available tide gauge data. We will also discuss issues dealing with refinement criteria, optimal resolution and refinement ratios, and inundation.

Mitigation of Electromagnetic Pulse (EMP) Effects from Short-Pulse Lasers and Fusion Neutrons

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eder, D C; Throop, A; Brown, Jr., C G

2009-03-06

Our research focused on obtaining a fundamental understanding of the source and properties of EMP at the Titan PW(petawatt)-class laser facility. The project was motivated by data loss and damage to components due to EMP, which can limit diagnostic techniques that can be used reliably at short-pulse PW-class laser facilities. Our measurements of the electromagnetic fields, using a variety of probes, provide information on the strength, time duration, and frequency dependence of the EMP. We measure electric field strengths in the 100's of kV/m range, durations up to 100 ns, and very broad frequency response extending out to 5 GHzmore » and possibly beyond. This information is being used to design shielding to mitigate the effects of EMP on components at various laser facilities. We showed the need for well-shielded cables and oscilloscopes to obtain high quality data. Significant work was invested in data analysis techniques to process this data. This work is now being transferred to data analysis procedures for the EMP diagnostics being fielded on the National Ignition Facility (NIF). In addition to electromagnetic field measurements, we measured the spatial and energy distribution of electrons escaping from targets. This information is used as input into the 3D electromagnetic code, EMSolve, which calculates time dependent electromagnetic fields. The simulation results compare reasonably well with data for both the strength and broad frequency bandwidth of the EMP. This modeling work required significant improvements in EMSolve to model the fields in the Titan chamber generated by electrons escaping the target. During dedicated Titan shots, we studied the effects of varying laser energy, target size, and pulse duration on EMP properties. We also studied the effect of surrounding the target with a thick conducting sphere and cube as a potential mitigation approach. System generated EMP (SGEMP) in coaxial cables does not appear to be a significant at Titan. Our results are directly relevant to planned short-pulse ARC (advanced radiographic capability) operation on NIF.« less

Generalized and efficient algorithm for computing multipole energies and gradients based on Cartesian tensors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lin, Dejun, E-mail: dejun.lin@gmail.com

2015-09-21

Accurate representation of intermolecular forces has been the central task of classical atomic simulations, known as molecular mechanics. Recent advancements in molecular mechanics models have put forward the explicit representation of permanent and/or induced electric multipole (EMP) moments. The formulas developed so far to calculate EMP interactions tend to have complicated expressions, especially in Cartesian coordinates, which can only be applied to a specific kernel potential function. For example, one needs to develop a new formula each time a new kernel function is encountered. The complication of these formalisms arises from an intriguing and yet obscured mathematical relation between themore » kernel functions and the gradient operators. Here, I uncover this relation via rigorous derivation and find that the formula to calculate EMP interactions is basically invariant to the potential kernel functions as long as they are of the form f(r), i.e., any Green’s function that depends on inter-particle distance. I provide an algorithm for efficient evaluation of EMP interaction energies, forces, and torques for any kernel f(r) up to any arbitrary rank of EMP moments in Cartesian coordinates. The working equations of this algorithm are essentially the same for any kernel f(r). Recently, a few recursive algorithms were proposed to calculate EMP interactions. Depending on the kernel functions, the algorithm here is about 4–16 times faster than these algorithms in terms of the required number of floating point operations and is much more memory efficient. I show that it is even faster than a theoretically ideal recursion scheme, i.e., one that requires 1 floating point multiplication and 1 addition per recursion step. This algorithm has a compact vector-based expression that is optimal for computer programming. The Cartesian nature of this algorithm makes it fit easily into modern molecular simulation packages as compared with spherical coordinate-based algorithms. A software library based on this algorithm has been implemented in C++11 and has been released.« less

Communicating Storm Surge Forecast Uncertainty

NASA Astrophysics Data System (ADS)

Troutman, J. A.; Rhome, J.

2015-12-01

When it comes to tropical cyclones, storm surge is often the greatest threat to life and property along the coastal United States. The coastal population density has dramatically increased over the past 20 years, putting more people at risk. Informing emergency managers, decision-makers and the public about the potential for wind driven storm surge, however, has been extremely difficult. Recently, the Storm Surge Unit at the National Hurricane Center in Miami, Florida has developed a prototype experimental storm surge watch/warning graphic to help communicate this threat more effectively by identifying areas most at risk for life-threatening storm surge. This prototype is the initial step in the transition toward a NWS storm surge watch/warning system and highlights the inundation levels that have a 10% chance of being exceeded. The guidance for this product is the Probabilistic Hurricane Storm Surge (P-Surge) model, which predicts the probability of various storm surge heights by statistically evaluating numerous SLOSH model simulations. Questions remain, however, if exceedance values in addition to the 10% may be of equal importance to forecasters. P-Surge data from 2014 Hurricane Arthur is used to ascertain the practicality of incorporating other exceedance data into storm surge forecasts. Extracting forecast uncertainty information through analyzing P-surge exceedances overlaid with track and wind intensity forecasts proves to be beneficial for forecasters and decision support.

Great Britain Storm Surge Modeling for a 10,000-Year Stochastic Catalog with the Effect of Sea Level Rise

NASA Astrophysics Data System (ADS)

Keshtpoor, M.; Carnacina, I.; Blair, A.; Yablonsky, R. M.

2017-12-01

Storm surge caused by Extratropical Cyclones (ETCs) has significantly impacted not only the life of private citizens but also the insurance and reinsurance industry in Great Britain. The storm surge risk assessment requires a larger dataset of storms than the limited recorded historical ETCs. Thus, historical ETCs were perturbed to generate a 10,000-year stochastic catalog that accounts for surge-generating ETCs in the study area with return periods from one year to 10,000 years. Delft3D-Flexible Mesh hydrodynamic model was used to numerically simulate the storm surge along the Great Britain coastline. A nested grid technique was used to increase the simulation grid resolution up to 200 m near the highly populated coastal areas. Coarse and fine mesh models were calibrated and validated using historical recorded water elevations. Then, numerical simulations were performed on a 10,000-year stochastic catalog. The 50-, 100-, and 500-year return period maps were generated for Great Britain coastal areas. The corresponding events with return periods of 50-, 100-, and 500-years in Humber Bay and Thames River coastal areas were identified, and simulated with the consideration of projected sea level rises to reveal the effect of rising sea levels on the inundation return period maps in two highly-populated coastal areas. Finally, the return period of Storm Xaver (2013) was determined with and without the effect of rising sea levels.

A Basis Function Approach to Simulate Storm Surge Events for Coastal Flood Risk Assessment

NASA Astrophysics Data System (ADS)

Wu, Wenyan; Westra, Seth; Leonard, Michael

2017-04-01

Storm surge is a significant contributor to flooding in coastal and estuarine regions, especially when it coincides with other flood producing mechanisms, such as extreme rainfall. Therefore, storm surge has always been a research focus in coastal flood risk assessment. Often numerical models have been developed to understand storm surge events for risk assessment (Kumagai et al. 2016; Li et al. 2016; Zhang et al. 2016) (Bastidas et al. 2016; Bilskie et al. 2016; Dalledonne and Mayerle 2016; Haigh et al. 2014; Kodaira et al. 2016; Lapetina and Sheng 2015), and assess how these events may change or evolve in the future (Izuru et al. 2015; Oey and Chou 2016). However, numeric models often require a lot of input information and difficulties arise when there are not sufficient data available (Madsen et al. 2015). Alternative, statistical methods have been used to forecast storm surge based on historical data (Hashemi et al. 2016; Kim et al. 2016) or to examine the long term trend in the change of storm surge events, especially under climate change (Balaguru et al. 2016; Oh et al. 2016; Rueda et al. 2016). In these studies, often the peak of surge events is used, which result in the loss of dynamic information within a tidal cycle or surge event (i.e. a time series of storm surge values). In this study, we propose an alternative basis function (BF) based approach to examine the different attributes (e.g. peak and durations) of storm surge events using historical data. Two simple two-parameter BFs were used: the exponential function and the triangular function. High quality hourly storm surge record from 15 tide gauges around Australia were examined. It was found that there are significantly location and seasonal variability in the peak and duration of storm surge events, which provides additional insights in coastal flood risk. In addition, the simple form of these BFs allows fast simulation of storm surge events and minimises the complexity of joint probability analysis for flood risk analysis considering multiple flood producing mechanisms. This is the first step in applying a Monte Carlo based joint probability method for flood risk assessment.

Storm surge and tide interaction: a complete paradigm

NASA Astrophysics Data System (ADS)

Horsburgh, Kevin; Williams, Jane; Proctor, Robert

2014-05-01

Globally, 200 million people live on coastal floodplains and about 1 trillion worth of assets lie within 1 metre of mean sea level. Any change in the statistics of flood frequency or severity would impact on economic and social systems. It is therefore crucial to understand the physical drivers of extreme storm surges, and to have confidence in datasets used for extreme sea level statistics. Much previous research has focussed on the process of tide-surge interaction, and it is now widely accepted that the physical basis of tide-surge interaction is that a phase shift of the tidal signal represents the effect of the surge on the tide. The second aspect of interaction is that shallow water momentum considerations imply that differing tidal states should modulate surge generation: wind stress should have greater surge-generating potential on lower tides. This has been shown previously by analytical models but not as yet confirmed by fully non-linear models of the continental shelf. We present results from an operational model of the European shelf that demonstrate that tidal range does have an effect on the surges generated. The cycle-integrated effects of wind stress (i.e. the skew surge) are generally greater when tidal range is low. Our results contradict the absence of any such correlation observed in the complete record of UK tide gauge data. This suggests that whilst the modulating effect of the tide on the skew surge (the time-independent difference between peak prediction and observations) is significant, the difference between individual storms is dominant. This implies that forecasting systems must predict salient detail of the most intense storms. A further implication is that operational models need to simulate tides with acceptable accuracy at all coastal locations. We extend our model analysis to show that the same modulation of storm surges (by tidal conditions) applies to tropical cyclones. We conduct simulations using a mature operational storm surge model in the Bay of Bengal with tropical cyclones from the IBTrACs database; we demonstrate that - just as with the extra-tropical case - higher storm surges on the Bangladesh coastline are generated during smaller tides.

TEMPS (Transportable EMP Simulator) Final Report. Volume 2. Appendixes

DTIC Science & Technology

1973-08-01

Electromagnetic pulse Pulser system Pulse generator 20. ASSTRPACT (Confirma an reverse ese It neessary and IdmntSIy by Weeck pmbff) This report...Research Institute I>, PIFR-372 A.1 INTRODUCTION The Transportable Electromagnetic Pulse Simulator (TEMPS) was built for the Harry Diamond Laboratories

The Value of Wetlands in Protecting Southeast Louisiana from Hurricane Storm Surges

PubMed Central

Barbier, Edward B.; Georgiou, Ioannis Y.; Enchelmeyer, Brian; Reed, Denise J.

2013-01-01

The Indian Ocean tsunami in 2004 and Hurricanes Katrina and Rita in 2005 have spurred global interest in the role of coastal wetlands and vegetation in reducing storm surge and flood damages. Evidence that coastal wetlands reduce storm surge and attenuate waves is often cited in support of restoring Gulf Coast wetlands to protect coastal communities and property from hurricane damage. Yet interdisciplinary studies combining hydrodynamic and economic analysis to explore this relationship for temperate marshes in the Gulf are lacking. By combining hydrodynamic analysis of simulated hurricane storm surges and economic valuation of expected property damages, we show that the presence of coastal marshes and their vegetation has a demonstrable effect on reducing storm surge levels, thus generating significant values in terms of protecting property in southeast Louisiana. Simulations for four storms along a sea to land transect show that surge levels decline with wetland continuity and vegetation roughness. Regressions confirm that wetland continuity and vegetation along the transect are effective in reducing storm surge levels. A 0.1 increase in wetland continuity per meter reduces property damages for the average affected area analyzed in southeast Louisiana, which includes New Orleans, by $99-$133, and a 0.001 increase in vegetation roughness decreases damages by $24-$43. These reduced damages are equivalent to saving 3 to 5 and 1 to 2 properties per storm for the average area, respectively. PMID:23536815

The value of wetlands in protecting southeast louisiana from hurricane storm surges.

PubMed

Barbier, Edward B; Georgiou, Ioannis Y; Enchelmeyer, Brian; Reed, Denise J

2013-01-01

The Indian Ocean tsunami in 2004 and Hurricanes Katrina and Rita in 2005 have spurred global interest in the role of coastal wetlands and vegetation in reducing storm surge and flood damages. Evidence that coastal wetlands reduce storm surge and attenuate waves is often cited in support of restoring Gulf Coast wetlands to protect coastal communities and property from hurricane damage. Yet interdisciplinary studies combining hydrodynamic and economic analysis to explore this relationship for temperate marshes in the Gulf are lacking. By combining hydrodynamic analysis of simulated hurricane storm surges and economic valuation of expected property damages, we show that the presence of coastal marshes and their vegetation has a demonstrable effect on reducing storm surge levels, thus generating significant values in terms of protecting property in southeast Louisiana. Simulations for four storms along a sea to land transect show that surge levels decline with wetland continuity and vegetation roughness. Regressions confirm that wetland continuity and vegetation along the transect are effective in reducing storm surge levels. A 0.1 increase in wetland continuity per meter reduces property damages for the average affected area analyzed in southeast Louisiana, which includes New Orleans, by $99-$133, and a 0.001 increase in vegetation roughness decreases damages by $24-$43. These reduced damages are equivalent to saving 3 to 5 and 1 to 2 properties per storm for the average area, respectively.

Concepts for Functional Restoration of Barrier Islands

DTIC Science & Technology

2009-09-01

percent. Restoration of the islands also delayed the peak of the surge by 2 hr as calculated landward of the Chandeleur Islands, Louisiana. Calculations... Chandeleur Islands. Wamsley et al. (2009) conducted similar simulations with coupled wave and circulation models for the Chandeleur Islands for two...simulations indicated that degradation of the Chandeleur Islands increased the peak storm surge slightly (less than 1.6 ft) at the hurricane

Developments in Surge Research Priorities: A Systematic Review of the Literature Following the Academic Emergency Medicine Consensus Conference, 2007-2015.

PubMed

Morton, Melinda J; DeAugustinis, Matthew L; Velasquez, Christina A; Singh, Sonal; Kelen, Gabor D

2015-11-01

In 2006, Academic Emergency Medicine (AEM) published a special issue summarizing the proceedings of the AEM consensus conference on the "Science of Surge." One major goal of the conference was to establish research priorities in the field of "disasters" surge. For this review, we wished to determine the progress toward the conference's identified research priorities: 1) defining criteria and methods for allocation of scarce resources, 2) identifying effective triage protocols, 3) determining decision-makers and means to evaluate response efficacy, 4) developing communication and information sharing strategies, and 5) identifying methods for evaluating workforce needs. Specific criteria were developed in conjunction with library search experts. PubMed, Embase, Web of Science, Scopus, and the Cochrane Library databases were queried for peer-reviewed articles from 2007 to 2015 addressing scientific advances related to the above five research priorities identified by AEM consensus conference. Abstracts and foreign language articles were excluded. Only articles with quantitative data on predefined outcomes were included; consensus panel recommendations on the above priorities were also included for the purposes of this review. Included study designs were randomized controlled trials, prospective, retrospective, qualitative (consensus panel), observational, cohort, case-control, or controlled before-and-after studies. Quality assessment was performed using a standardized tool for quantitative studies. Of the 2,484 unique articles identified by the search strategy, 313 articles appeared to be related to disaster surge. Following detailed text review, 50 articles with quantitative data and 11 concept papers (consensus conference recommendations) addressed at least one AEM consensus conference surge research priority. Outcomes included validation of the benchmark of 500 beds/million of population for disaster surge capacity, effectiveness of simulation- and Internet-based tools for forecasting of hospital and regional demand during disasters, effectiveness of reverse triage approaches, development of new disaster surge metrics, validation of mass critical care approaches (altered standards of care), use of telemedicine, and predictions of optimal hospital staffing levels for disaster surge events. Simulation tools appeared to provide some of the highest quality research. Disaster simulation studies have arguably revolutionized the study of disaster surge in the intervening years since the 2006 AEM Science of Surge conference, helping to validate some previously known disaster surge benchmarks and to generate new surge metrics. Use of reverse triage approaches and altered standards of care, as well as Internet-based tools such as Google Flu Trends, have also proven effective. However, there remains significant work to be done toward standardizing research methodologies and outcomes, as well as validating disaster surge metrics. © 2015 by the Society for Academic Emergency Medicine.

Numerical simulation of pounding damage to caisson under storm surge

NASA Astrophysics Data System (ADS)

Yu, Chen

2018-06-01

In this paper, a new method for the numerical simulation of structural model is proposed, which is employed to analyze the pounding response of caissons subjected to storm surge loads. According to the new method, the simulation process is divided into two steps. Firstly, the wave propagation caused by storm surge is simulated by the wave-generating tool of Flow-3D, and recording the wave force time history on the caisson. Secondly, a refined 3D finite element model of caisson is established, and the wave force load is applied on the caisson according to the measured data in the first step for further analysis of structural pounding response using the explicit solver of LSDYNA. The whole simulation of pounding response of a caisson caused by "Sha Lijia" typhoon is carried out. The results show that the different wave direction results in the different angle caisson collisions, which will lead to different failure mode of caisson, and when the angle of 60 between wave direction and front/back wall is simulated, the numerical pounding failure mode is consistent with the situation.

Probabilistic storm surge inundation maps for Metro Manila based on Philippine public storm warning signals

NASA Astrophysics Data System (ADS)

Tablazon, J.; Caro, C. V.; Lagmay, A. M. F.; Briones, J. B. L.; Dasallas, L.; Lapidez, J. P.; Santiago, J.; Suarez, J. K.; Ladiero, C.; Gonzalo, L. A.; Mungcal, M. T. F.; Malano, V.

2015-03-01

A storm surge is the sudden rise of sea water over the astronomical tides, generated by an approaching storm. This event poses a major threat to the Philippine coastal areas, as manifested by Typhoon Haiyan on 8 November 2013. This hydro-meteorological hazard is one of the main reasons for the high number of casualties due to the typhoon, with 6300 deaths. It became evident that the need to develop a storm surge inundation map is of utmost importance. To develop these maps, the Nationwide Operational Assessment of Hazards under the Department of Science and Technology (DOST-Project NOAH) simulated historical tropical cyclones that entered the Philippine Area of Responsibility. The Japan Meteorological Agency storm surge model was used to simulate storm surge heights. The frequency distribution of the maximum storm surge heights was calculated using simulation results of tropical cyclones under a specific public storm warning signal (PSWS) that passed through a particular coastal area. This determines the storm surge height corresponding to a given probability of occurrence. The storm surge heights from the model were added to the maximum astronomical tide data from WXTide software. The team then created maps of inundation for a specific PSWS using the probability of exceedance derived from the frequency distribution. Buildings and other structures were assigned a probability of exceedance depending on their occupancy category, i.e., 1% probability of exceedance for critical facilities, 10% probability of exceedance for special occupancy structures, and 25% for standard occupancy and miscellaneous structures. The maps produced show the storm-surge-vulnerable areas in Metro Manila, illustrated by the flood depth of up to 4 m and extent of up to 6.5 km from the coastline. This information can help local government units in developing early warning systems, disaster preparedness and mitigation plans, vulnerability assessments, risk-sensitive land use plans, shoreline defense efforts, and coastal protection measures. These maps can also determine the best areas to build critical structures, or at least determine the level of protection of these structures should they be built in hazard areas. Moreover, these will support the local government units' mandate to raise public awareness, disseminate information about storm surge hazards, and implement appropriate countermeasures for a given PSWS.

Predicting typhoon-induced storm surge tide with a two-dimensional hydrodynamic model and artificial neural network model

NASA Astrophysics Data System (ADS)

Chen, W.-B.; Liu, W.-C.; Hsu, M.-H.

2012-12-01

Precise predictions of storm surges during typhoon events have the necessity for disaster prevention in coastal seas. This paper explores an artificial neural network (ANN) model, including the back propagation neural network (BPNN) and adaptive neuro-fuzzy inference system (ANFIS) algorithms used to correct poor calculations with a two-dimensional hydrodynamic model in predicting storm surge height during typhoon events. The two-dimensional model has a fine horizontal resolution and considers the interaction between storm surges and astronomical tides, which can be applied for describing the complicated physical properties of storm surges along the east coast of Taiwan. The model is driven by the tidal elevation at the open boundaries using a global ocean tidal model and is forced by the meteorological conditions using a cyclone model. The simulated results of the hydrodynamic model indicate that this model fails to predict storm surge height during the model calibration and verification phases as typhoons approached the east coast of Taiwan. The BPNN model can reproduce the astronomical tide level but fails to modify the prediction of the storm surge tide level. The ANFIS model satisfactorily predicts both the astronomical tide level and the storm surge height during the training and verification phases and exhibits the lowest values of mean absolute error and root-mean-square error compared to the simulated results at the different stations using the hydrodynamic model and the BPNN model. Comparison results showed that the ANFIS techniques could be successfully applied in predicting water levels along the east coastal of Taiwan during typhoon events.

Time-resolved absolute measurements by electro-optic effect of giant electromagnetic pulses due to laser-plasma interaction in nanosecond regime

PubMed Central

Consoli, F.; De Angelis, R.; Duvillaret, L.; Andreoli, P. L.; Cipriani, M.; Cristofari, G.; Di Giorgio, G.; Ingenito, F.; Verona, C.

2016-01-01

We describe the first electro-optical absolute measurements of electromagnetic pulses (EMPs) generated by laser-plasma interaction in nanosecond regime. Laser intensities are inertial-confinement-fusion (ICF) relevant and wavelength is 1054 nm. These are the first direct EMP amplitude measurements with the detector rather close and in direct view of the plasma. A maximum field of 261 kV/m was measured, two orders of magnitude higher than previous measurements by conductive probes on nanosecond regime lasers with much higher energy. The analysis of measurements and of particle-in-cell simulations indicates that signals match the emission of charged particles detected in the same experiment, and suggests that anisotropic particle emission from target, X-ray photoionization and charge implantation on surfaces directly exposed to plasma, could be important EMP contributions. Significant information achieved on EMP features and sources is crucial for future plants of laser-plasma acceleration and inertial-confinement-fusion and for the use as effective plasma diagnostics. It also opens to remarkable applications of laser-plasma interaction as intense source of RF-microwaves for studies on materials and devices, EMP-radiation-hardening and electromagnetic compatibility. The demonstrated extreme effectivity of electric-fields detection in laser-plasma context by electro-optic effect, leads to great potential for characterization of laser-plasma interaction and generated Terahertz radiation. PMID:27301704

Time-resolved absolute measurements by electro-optic effect of giant electromagnetic pulses due to laser-plasma interaction in nanosecond regime

NASA Astrophysics Data System (ADS)

Consoli, F.; de Angelis, R.; Duvillaret, L.; Andreoli, P. L.; Cipriani, M.; Cristofari, G.; di Giorgio, G.; Ingenito, F.; Verona, C.

2016-06-01

We describe the first electro-optical absolute measurements of electromagnetic pulses (EMPs) generated by laser-plasma interaction in nanosecond regime. Laser intensities are inertial-confinement-fusion (ICF) relevant and wavelength is 1054 nm. These are the first direct EMP amplitude measurements with the detector rather close and in direct view of the plasma. A maximum field of 261 kV/m was measured, two orders of magnitude higher than previous measurements by conductive probes on nanosecond regime lasers with much higher energy. The analysis of measurements and of particle-in-cell simulations indicates that signals match the emission of charged particles detected in the same experiment, and suggests that anisotropic particle emission from target, X-ray photoionization and charge implantation on surfaces directly exposed to plasma, could be important EMP contributions. Significant information achieved on EMP features and sources is crucial for future plants of laser-plasma acceleration and inertial-confinement-fusion and for the use as effective plasma diagnostics. It also opens to remarkable applications of laser-plasma interaction as intense source of RF-microwaves for studies on materials and devices, EMP-radiation-hardening and electromagnetic compatibility. The demonstrated extreme effectivity of electric-fields detection in laser-plasma context by electro-optic effect, leads to great potential for characterization of laser-plasma interaction and generated Terahertz radiation.

Numerical simulation of narrow bipolar electromagnetic pulses generated by thunderstorm discharges

NASA Astrophysics Data System (ADS)

Bochkov, E. I.; Babich, L. P.; Kutsyk, I. M.

2013-07-01

Using the concept of avalanche relativistic runaway electrons (REs), we perform numerical simulations of compact intracloud discharge (CID) as a generator of powerful natural electromagnetic pulses (EMPs) in the HF-VHF range, called narrow bipolar pulses (NBPs). For several values of the field overvoltage and altitude at which the discharge develops, the numbers of seed electrons initiating the avalanche are evaluated, with which the calculated EMP characteristics are consistent with the measured NBP parameters. We note shortcomings in the hypothesis assuming participation of cosmic ray air showers in avalanche initiation. The discharge capable of generating NBPs produces REs in numbers close to those in the source of terrestrial γ-ray flashes (TGFs), which can be an argument in favor of a unified NBP and TGF source.

On using scatterometer and altimeter data to improve storm surge forecasting in the Adriatic Sea

NASA Astrophysics Data System (ADS)

Bajo, Marco; Umgiesser, Georg; De Biasio, Francesco; Vignudelli, Stefano; Zecchetto, Stefano

2017-04-01

Satellite data are seldom used in storm surge forecasting. Among the most important issues related to the storm surge forecasting are the quality of the model wind forcing and the initial condition of the sea surface elevation. In this work, focused on storm surge forecasting in the Adriatic Sea, satellite scatterometer wind data are used to correct the wind speed and direction biases of the ECMWF global atmospheric model by tuning the spatial fields, as an alternative to data assimilation. The capability of such an unbiased wind is tested against that of a high resolution wind, produced by a regional non-hydrostatic model. On the other hand, altimeter Total Water Level Envelope (TWLE) data, which provide the sea level elevation, are used to improve the accuracy of the initial state of the model simulations. This is done by assimilating into a storm surge model the TWLE obtained by the altimeter observations along ground tracks, after subtraction of the tidal components. In order to test the methodology, eleven storm surge events recorded in Venice, from 2008 to 2012, have been simulated using different configurations of forcing wind and altimeter data assimilation. Results show that the relative error on the estimation of the maximum surge peak, averaged over the cases considered, decreases from 13% to 7% using both the unbiased wind and the altimeter data assimilation, while forcing the hydrodynamic model with the high resolution wind (no tuning), the altimeter data assimilation reduces the error from 9% to 6%.

Impact of using scatterometer and altimeter data on storm surge forecasting

NASA Astrophysics Data System (ADS)

Bajo, Marco; De Biasio, Francesco; Umgiesser, Georg; Vignudelli, Stefano; Zecchetto, Stefano

2017-05-01

Satellite data are rarely used in storm surge models because of the lack of established methodologies. Nevertheless, they can provide useful information on surface wind and sea level, which can potentially improve the forecast. In this paper satellite wind data are used to correct the bias of wind originating from a global atmospheric model, while satellite sea level data are used to improve the initial conditions of the model simulations. In a first step, the capability of global winds (biased and unbiased) to adequately force a storm surge model are assessed against that of a high resolution local wind. Then, the added value of direct assimilation of satellite altimeter data in the storm surge model is tested. Eleven storm surge events, recorded in Venice from 2008 to 2012, are simulated using different configurations of wind forcing and altimeter data assimilation. Focusing on the maximum surge peak, results show that the relative error, averaged over the eleven cases considered, decreases from 13% to 7%, using both the unbiased wind and assimilating the altimeter data, while, if the high resolution local wind is used to force the hydrodynamic model, the altimeter data assimilation reduces the error from 9% to 6%. Yet, the overall capabilities in reproducing the surge in the first day of forecast, measured by the correlation and by the rms error, improve only with the use of the unbiased global wind and not with the use of high resolution local wind and altimeter data assimilation.

Turbofan Engine Post-Instability Behavior - Computer Simulations, Test Validation, and Application of Simulations,

DTIC Science & Technology

COMPRESSORS, *AIR FLOW, TURBOFAN ENGINES , TRANSIENTS, SURGES, STABILITY, COMPUTERIZED SIMULATION, EXPERIMENTAL DATA, VALIDATION, DIGITAL SIMULATION, INLET GUIDE VANES , ROTATION, STALLING, RECOVERY, HYSTERESIS

Non-Impact, Blast-Induced Mild TBI and PTSD: Concepts and Caveats

DTIC Science & Technology

2011-07-01

has been verified by wound ballistics experiments in animals and finite element simulation of blast loads on the torso. Blood surge caused by...ballistic pressure waves in animals An experimental study of wound ballistics demon- strates that a ballistic pressure wave can cause a remote injury to...surge. This hypothesis has been supported by some experimental data. A volumetric surge of blood moved through the thorax and abdomen has been observed

The influence of sea surface temperature on the intensity and associated storm surge of tropical cyclone Yasi: a sensitivity study

NASA Astrophysics Data System (ADS)

Lavender, Sally L.; Hoeke, Ron K.; Abbs, Deborah J.

2018-03-01

Tropical cyclones (TCs) result in widespread damage associated with strong winds, heavy rainfall and storm surge. TC Yasi was one of the most powerful TCs to impact the Queensland coast since records began. Prior to Yasi, the SSTs in the Coral Sea were higher than average by 1-2 °C, primarily due to the 2010/2011 La Niña event. In this study, a conceptually simple idealised sensitivity analysis is performed using a high-resolution regional model to gain insight into the influence of SST on the track, size, intensity and associated rainfall of TC Yasi. A set of nine simulations with uniform SST anomalies of between -4 and 4 °C applied to the observed SSTs are analysed. The resulting surface winds and pressure are used to force a barotropic storm surge model to examine the influence of SST on the associated storm surge of TC Yasi. An increase in SST results in an increase in intensity, precipitation and integrated kinetic energy of the storm; however, there is little influence on track prior to landfall. In addition to an increase in precipitation, there is a change in the spatial distribution of precipitation as the SST increases. Decreases in SSTs result in an increase in the radius of maximum winds due to an increase in the asymmetry of the storm, although the radius of gale-force winds decreases. These changes in the TC characteristics also lead to changes in the associated storm surge. Generally, cooler (warmer) SSTs lead to reduced (enhanced) maximum storm surges. However, the increase in surge reaches a maximum with an increase in SST of 2 °C. Any further increase in SST does not affect the maximum surge but the total area and duration of the simulated surge increases with increasing upper ocean temperatures. A large decrease in maximum storm surge height occurs when a negative SST anomaly is applied, suggesting if TC Yasi had occurred during non-La Niña conditions the associated storm surge may have been greatly diminished, with a decrease in storm surge height of over 3 m when the SST is reduced by 2 °C. In summary, increases in SST lead to an increase in the potential destructiveness of TCs with regard to intensity, precipitation and storm surge, although this relationship is not linear.

Assessment of water pipes durability under pressure surge

NASA Astrophysics Data System (ADS)

Pham Ha, Hai; Minh, Lanh Pham Thi; Tang Van, Lam; Bulgakov, Boris; Bazhenova, Soafia

2017-10-01

Surge phenomenon occurs on the pipeline by the closing valve or pump suddenly lost power. Due to the complexity of the water hammer simulation, previous researches have only considered water hammer on the single pipe or calculation of some positions on water pipe network, it have not been analysis for all of pipe on the water distribution systems. Simulation of water hammer due to closing valve on water distribution system and the influence level of pressure surge is evaluated at the defects on pipe. Water hammer on water supply pipe network are simulated by Water HAMMER software academic version and the capacity of defects are calculated by SINTAP. SINTAP developed from Brite-Euram projects in Brussels-Belgium with the aim to develop a process for assessing the integrity of the structure for the European industry. Based on the principle of mechanical fault, indicating the size of defects in materials affect the load capacity of the product in the course of work, the process has proposed setting up the diagram to fatigue assessment defect (FAD). The methods are applied for water pipe networks of Lien Chieu district, Da Nang city, Viet Nam, the results show the affected area of wave pressure by closing the valve and thereby assess the greatest pressure surge effect to corroded pipe. The SINTAP standard and finite element mesh analysis at the defect during the occurrence of pressure surge which will accurately assess the bearing capacity of the old pipes. This is one of the bases to predict the leakage locations on the water distribution systems. Amount of water hammer when identified on the water supply networks are decreasing due to local losses at the nodes as well as the friction with pipe wall, so this paper adequately simulate water hammer phenomena applying for actual water distribution systems. The research verified that pipe wall with defect is damaged under the pressure surge value.

Measurement and numerical simulation of a small centrifugal compressor characteristics at small or negative flow rate

NASA Astrophysics Data System (ADS)

Tsukamoto, Kaname; Okada, Mizuki; Inokuchi, Yuzo; Yamasaki, Nobuhiko; Yamagata, Akihiro

2017-04-01

For centrifugal compressors used in automotive turbochargers, the extension of the surge margin is demanded because of lower engine speed. In order to estimate the surge line exactly, it is required to acquire the compressor characteristics at small or negative flow rate. In this paper, measurement and numerical simulation of the characteristics at small or negative flow rate are carried out. In the measurement, an experimental facility with a valve immediately downstream of the compressor is used to suppress the surge. In the numerical work, a new boundary condition that specifies mass flow rate at the outlet boundary is used to simulate the characteristics around the zero flow rate region. Furthermore, flow field analyses at small or negative flow rate are performed with the numerical results. The separated and re-circulated flow fields are investigated by visualization to identify the origin of losses.

Numerical Evaluation of Storm Surge Indices for Public Advisory Purposes

NASA Astrophysics Data System (ADS)

Bass, B.; Bedient, P. B.; Dawson, C.; Proft, J.

2016-12-01

After the devastating hurricane season of 2005, shortcomings with the Saffir-Simpson Hurricane Scale's (SSHS) ability to characterize a tropical cyclones potential to generate storm surge became widely apparent. As a result, several alternative surge indices were proposed to replace the SSHS, including Powell and Reinhold's Integrated Kinetic Energy (IKE) factor, Kantha's Hurricane Surge Index (HSI), and Irish and Resio's Surge Scale (SS). Of the previous, the IKE factor is the only surge index to-date that truly captures a tropical cyclones integrated intensity, size, and wind field distribution. However, since the IKE factor was proposed in 2007, an accurate assessment of this surge index has not been performed. This study provides the first quantitative evaluation of the IKEs ability to serve as a predictor of a tropical cyclones potential surge impacts as compared to other alternative surge indices. Using the tightly coupled ADvanced CIRCulation and Simulating WAves Nearshore models, the surge and wave responses of Hurricane Ike (2008) and 78 synthetic tropical cyclones were evaluated against the SSHS, IKE, HSI and SS. Results along the upper TX coast of the Gulf of Mexico demonstrate that the HSI performs best in capturing the peak surge response of a tropical cyclone, while the IKE accounting for winds greater than tropical storm intensity (IKETS) provides the most accurate estimate of a tropical cyclones regional surge impacts. These results demonstrate that the appropriate selection of a surge index ultimately depends on what information is of interest to be conveyed to the public and/or scientific community.

Multidecadal Scale Detection Time for Potentially Increasing Atlantic Storm Surges in a Warming Climate

NASA Astrophysics Data System (ADS)

Lee, Benjamin Seiyon; Haran, Murali; Keller, Klaus

2017-10-01

Storm surges are key drivers of coastal flooding, which generate considerable risks. Strategies to manage these risks can hinge on the ability to (i) project the return periods of extreme storm surges and (ii) detect potential changes in their statistical properties. There are several lines of evidence linking rising global average temperatures and increasingly frequent extreme storm surges. This conclusion is, however, subject to considerable structural uncertainty. This leads to two main questions: What are projections under various plausible statistical models? How long would it take to distinguish among these plausible statistical models? We address these questions by analyzing observed and simulated storm surge data. We find that (1) there is a positive correlation between global mean temperature rise and increasing frequencies of extreme storm surges; (2) there is considerable uncertainty underlying the strength of this relationship; and (3) if the frequency of storm surges is increasing, this increase can be detected within a multidecadal timescale (≈20 years from now).

Compound simulation of fluvial floods and storm surges in a global coupled river-coast flood model: Model development and its application to 2007 Cyclone Sidr in Bangladesh

NASA Astrophysics Data System (ADS)

Ikeuchi, Hiroaki; Hirabayashi, Yukiko; Yamazaki, Dai; Muis, Sanne; Ward, Philip J.; Winsemius, Hessel C.; Verlaan, Martin; Kanae, Shinjiro

2017-08-01

Water-related disasters, such as fluvial floods and cyclonic storm surges, are a major concern in the world's mega-delta regions. Furthermore, the simultaneous occurrence of extreme discharges from rivers and storm surges could exacerbate flood risk, compared to when they occur separately. Hence, it is of great importance to assess the compound risks of fluvial and coastal floods at a large scale, including mega-deltas. However, most studies on compound fluvial and coastal flooding have been limited to relatively small scales, and global-scale or large-scale studies have not yet addressed both of them. The objectives of this study are twofold: to develop a global coupled river-coast flood model; and to conduct a simulation of compound fluvial flooding and storm surges in Asian mega-delta regions. A state-of-the-art global river routing model was modified to represent the influence of dynamic sea surface levels on river discharges and water levels. We conducted the experiments by coupling a river model with a global tide and surge reanalysis data set. Results show that water levels in deltas and estuaries are greatly affected by the interaction between river discharge, ocean tides and storm surges. The effects of storm surges on fluvial flooding are further examined from a regional perspective, focusing on the case of Cyclone Sidr in the Ganges-Brahmaputra-Meghna Delta in 2007. Modeled results demonstrate that a >3 m storm surge propagated more than 200 km inland along rivers. We show that the performance of global river routing models can be improved by including sea level dynamics.

Explicitly represented polygon wall boundary model for the explicit MPS method

NASA Astrophysics Data System (ADS)

Mitsume, Naoto; Yoshimura, Shinobu; Murotani, Kohei; Yamada, Tomonori

2015-05-01

This study presents an accurate and robust boundary model, the explicitly represented polygon (ERP) wall boundary model, to treat arbitrarily shaped wall boundaries in the explicit moving particle simulation (E-MPS) method, which is a mesh-free particle method for strong form partial differential equations. The ERP model expresses wall boundaries as polygons, which are explicitly represented without using the distance function. These are derived so that for viscous fluids, and with less computational cost, they satisfy the Neumann boundary condition for the pressure and the slip/no-slip condition on the wall surface. The proposed model is verified and validated by comparing computed results with the theoretical solution, results obtained by other models, and experimental results. Two simulations with complex boundary movements are conducted to demonstrate the applicability of the E-MPS method to the ERP model.

The cause of outliers in electromagnetic pulse (EMP) locations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fenimore, Edward E.

2014-10-02

We present methods to calculate the location of EMP pulses when observed by 5 or more satellites. Simulations show that, even with a good initial guess and fitting a location to all of the data, there are sometime outlier results whose locations are much worse than most cases. By comparing simulations using different ionospheric transfer functions (ITFs), it appears that the outliers are caused by not including the additional path length due to refraction rather than being caused by not including higher order terms in the Appleton-Hartree equation. We suggest ways that the outliers can be corrected. These correction methodsmore » require one to use an electron density profile along the line of sight from the event to the satellite rather than using the total electron content (TEC) to characterize the ionosphere.« less

The effects of climate change on storm surges around the United Kingdom.

PubMed

Lowe, J A; Gregory, J M

2005-06-15

Coastal flooding is often caused by extreme events, such as storm surges. In this study, improved physical models have been used to simulate the climate system and storm surges, and to predict the effect of increased atmospheric concentrations of greenhouse gases on the surges. In agreement with previous studies, this work indicates that the changes in atmospheric storminess and the higher time-average sea-level predicted for the end of the twenty-first century will lead to changes in the height of water levels measured relative to the present day tide. However, the details of these projections differ somewhat from earlier assessments. Uncertainty in projections of future extreme water levels arise from uncertainty in the amount and timing of future greenhouse gas emissions, uncertainty in the physical models used to simulate the climate system and from the natural variability of the system. The total uncertainty has not yet been reliably quantified and achieving this should be a priority for future research.

The Effect of Coastal Development on Storm Surge Flooding in Biscayne Bay, Florida, USA (Invited)

NASA Astrophysics Data System (ADS)

Zhang, K.; Liu, H.; Li, Y.

2013-12-01

Barrier islands and associated bays along the Atlantic and Gulf Coasts are a favorite place for both living and visiting. Many of them are vulnerable to storm surge flooding because of low elevations and constantly being subjected to the impacts of storms. The population increase and urban development along the barrier coast have altered the shoreline configuration, resulting in a dramatic change in the coastal flooding pattern in some areas. Here we present such a case based on numerical simulations of storm surge flooding caused by the1926 hurricane in the densely populated area surrounding Biscayne Bay in Miami, Florida. The construction of harbor and navigation channels, and the development of real estate and the roads connecting islands along Biscayne Bay have changed the geometry of Biscayne Bay since 1910s. Storm surge simulations show that the Port of Miami and Dodge Island constructed by human after 1950 play an important role in changing storm surge inundation pattern along Biscayne Bay. Dodge Island enhances storm surge and increases inundation in the area south of the island, especially at the mouth of Miami River (Downtown of Miami), and reduces storm surge flooding in the area north of the island, especially in Miami Beach. If the Hurricane Miami of 1926 happened today, the flooding area would be reduced by 55% and 20% in the Miami Beach and North Miami areas, respectively. Consequently, it would prevent 400 million of property and 10 thousand people from surge flooding according to 2010 U.S census and 2007 property tax data. Meanwhile, storm water would penetrate further inland south of Dodge Island and increase the flooding area by 25% in the Miami River and Downtown Miami areas. As a result, 200 million of property and five thousand people would be impacted by storm surge.

Sinonasal extramedullary plasmacytoma: a population-based incidence and survival analysis.

PubMed

Patel, Tapan D; Vázquez, Alejandro; Choudhary, Moaz M; Kam, David; Baredes, Soly; Eloy, Jean Anderson

2015-09-01

Sinonasal extramedullary plasmacytoma (SN-EMP) is a rare plasma cell neoplasm. Published literature on this tumor largely consists of case reports and case-series with small sample sizes. This study analyzed population-based data on SN-EMP patients to understand demographic and clinical features as well as incidence and survival trends. The Surveillance, Epidemiology, and End Results (SEER) database was queried for SN-EMP and other head and neck EMP (HN-EMP) cases from 1973 to 2011. Cases were analyzed to determine patient demographics, initial treatment modality, and survival outcomes. Of 778 patients identified with EMP in the head and neck region, 367 patients had SN-EMP and 411 had other HN-EMP. There was a strong male predilection found, with a male-to-female ratio of 3.65:1 in the SN-EMP group and 1.87:1 in the other HN-EMP group. The majority of the patients presented with localized disease in both SN-EMP (84.4%) and other HN-EMP (81.0%) groups. The most common treatment modality reported in this database was surgery with adjuvant radiotherapy in both SN-EMP (46.3%) and other HN-EMP (38.9%) groups, followed by radiotherapy alone (SN-EMP: 40.7%; other HN-EMP: 34.2%). Five-year and 10-year disease-specific survival rates were comparable between SN-EMP (88.2% and 83.3%, respectively) and other HN-EMP (90.0% and 87.4%, respectively) (p = 0.6016 and p = 0.4015, respectively). This study analyzed the largest cohort of SN-EMP patients to date. There was no statistically significant survival advantage found for any 1 particular treatment modality over other treatment modalities in both SN-EMP and other HN-EMP. © 2015 ARS-AAOA, LLC.

Endothelial microparticles (EMP) bind and activate monocytes: elevated EMP-monocyte conjugates in multiple sclerosis.

PubMed

Jy, Wenche; Minagar, Alireza; Jimenez, Joaquin J; Sheremata, William A; Mauro, Lucia M; Horstman, Lawrence L; Bidot, Carlos; Ahn, Yeon S

2004-09-01

Elevated plasma endothelial microparticles (EMP) have been documented in MS during exacerbation. However, the role of EMP in pathogenesis of MS remains unclear. We investigated the formation of EMP-monocyte conjugates (EMP-MoC) and their potential role in transendothelial migration of inflammatory cells in MS. EMP-MoC were assayed in 30 MS patients in exacerbation, 20 in remission and in 35 controls. EMP-leukocyte conjugation was investigated flowcytometrically by employing alpha-CD54 or alpha-CD62E for EMP, and alpha-CD45 for leukocytes. EMP-MoC were characterized by identifying adhesion molecules involved and their effect on monocyte function. In vivo (clinical): EMP-MoC were markedly elevated in exacerbation vs. remission and controls, correlating with presence of GD+ MRI lesions. Free CD54+ EMP were not elevated but free CD62E+ EMP were. In vitro: EMP bound preferentially to monocytes, less to neutrophils, but little to lymphocytes. Bound EMP activated monocytes: CD11b expression increased 50% and migration through cerebral endothelial cell layer increased 2.6-fold. Blockade of CD54 reduced binding by 80%. Most CD54+ EMP bound to monocytes, leaving little free EMP, while CD62+ EMP were found both free and bound. These results demonstrated that phenotypic subsets of EMP interacted differently with monocytes. Based on our observations, EMP may enhance inflammation and increase transendothelial migration of monocytes in MS by binding to and activating monocytes through CD54. EMP-MoC were markedly increased in MS patients in exacerbation compared to remission and may serve as a sensitive marker of MS disease activity.

Dynamic simulation and numerical analysis of hurricane storm surge under sea level rise with geomorphologic changes along the northern Gulf of Mexico

USGS Publications Warehouse

Bilskie, Matthew V.; Hagen, S.C.; Alizad, K.A.; Medeiros, S.C.; Passeri, Davina L.; Needham, H.F.; Cox, A.

2016-01-01

This work outlines a dynamic modeling framework to examine the effects of global climate change, and sea level rise (SLR) in particular, on tropical cyclone-driven storm surge inundation. The methodology, applied across the northern Gulf of Mexico, adapts a present day large-domain, high resolution, tide, wind-wave, and hurricane storm surge model to characterize the potential outlook of the coastal landscape under four SLR scenarios for the year 2100. The modifications include shoreline and barrier island morphology, marsh migration, and land use land cover change. Hydrodynamics of 10 historic hurricanes were simulated through each of the five model configurations (present day and four SLR scenarios). Under SLR, the total inundated land area increased by 87% and developed and agricultural lands by 138% and 189%, respectively. Peak surge increased by as much as 1 m above the applied SLR in some areas, and other regions were subject to a reduction in peak surge, with respect to the applied SLR, indicating a nonlinear response. Analysis of time-series water surface elevation suggests the interaction between SLR and storm surge is nonlinear in time; SLR increased the time of inundation and caused an earlier arrival of the peak surge, which cannot be addressed using a static (“bathtub”) modeling framework. This work supports the paradigm shift to using a dynamic modeling framework to examine the effects of global climate change on coastal inundation. The outcomes have broad implications and ultimately support a better holistic understanding of the coastal system and aid restoration and long-term coastal sustainability.

Role of the Emp Pilus Subunits of Enterococcus faecium in Biofilm Formation, Adherence to Host Extracellular Matrix Components, and Experimental Infection

PubMed Central

Montealegre, Maria Camila; Singh, Kavindra V.; Somarajan, Sudha R.; Yadav, Puja; Chang, Chungyu; Spencer, Robert; Sillanpää, Jouko; Ton-That, Hung

2016-01-01

Enterococcus faecium is an important cause of hospital-associated infections, including urinary tract infections (UTIs), bacteremia, and infective endocarditis. Pili have been shown to play a role in the pathogenesis of Gram-positive bacteria, including E. faecium. We previously demonstrated that a nonpiliated ΔempABC::cat derivative of E. faecium TX82 was attenuated in biofilm formation and in a UTI model. Here, we studied the contributions of the individual pilus subunits EmpA, EmpB, and EmpC to pilus architecture, biofilm formation, adherence to extracellular matrix (ECM) proteins, and infection. We identified EmpA as the tip of the pili and found that deletion of empA reduced biofilm formation to the same level as deletion of the empABC operon, a phenotype that was restored by reconstituting in situ the empA gene. Deletion of empB also caused a reduction in biofilm, while EmpC was found to be dispensable. Significant reductions in adherence to fibrinogen and collagen type I were observed with deletion of empA and empB, while deletion of empC had no adherence defect. Furthermore, we showed that each deletion mutant was significantly attenuated in comparison to the isogenic parental strain, TX82, in a mixed-inoculum UTI model (P < 0.001 to 0.048), that reconstitution of empA restored virulence in the UTI model, and that deletion of empA also resulted in attenuation in an infective endocarditis model (P = 0.0088). Our results indicate that EmpA and EmpB, but not EmpC, contribute to biofilm and adherence to ECM proteins; however, all the Emp pilins are important for E. faecium to cause infection in the urinary tract. PMID:26930703

Impacts of land cover changes on hurricane storm surge in the lower Chesapeake Bay

NASA Astrophysics Data System (ADS)

Denton, M.; Lawler, S.; Ferreira, C.

2013-12-01

The Chesapeake Bay is the largest estuary in the United States with more than 150 rivers draining into the bay's tidal wetlands. Coastal wetlands and vegetation play an important role in shaping the hydrodynamics of storm surge events by retaining water and slowing the propagation of storm surge. In this way coastal wetlands act as a natural barrier to inland flooding, particularly against less intense storms. Threats to wetlands come from both land development (residential or commercial/industrial) and sea level rise. The lower region of the Chesapeake Bay near its outlet is especially vulnerable to flooding from Atlantic storm surge brought in by hurricanes, tropical storms and nor'easters (e.g., hurricanes Isabel [2003] and Sandy [2012]). This region is also intensely developed with nearly 1.7 million residents within the greater Hampton Roads metropolitan area. Anthropogenic changes to land cover in the lower bay can directly impact basin drainage and storm surge propagation with impacts reaching beyond the immediate coastal zone to affect flooding in inland areas. While construction of seawall barriers around population centers may provide storm surge protection to a specifically defined area, these barriers deflect storm surge rather than attenuate it, underscoring the importance of wetlands. To analyze these impacts a framework was developed combining numerical simulations with a detailed hydrodynamic characterization of flow through coastal wetland areas. Storm surges were calculated using a hydrodynamic model (ADCIRC) coupled to a wave model (SWAN) forced by an asymmetric hurricane vortex model using the FEMA region 3 unstructured mesh (2.3 million nodes) under a High Performance Computing (HPC) environment. Multiple model simulations were performed using historical hurricanes data and hypothetical storms to compare the predicted storm surge inundation with various levels of wetland reduction and/or beach hardening. These data were combined and overlaid with a geospatial inventory of critical infrastructure assets to evaluate the potential for storm damage associated with each level of wetland reduction. This poster will present quantitative analyses of the benefits and losses regarding storm surge inundation and damage from land cover changes in the study region.

Projected Sea Level Rise and Changes in Extreme Storm Surge and Wave Events During the 21st Century in the Region of Singapore

NASA Astrophysics Data System (ADS)

Palmer, M. D.; Cannaby, H.; Howard, T.; Bricheno, L.

2016-02-01

Singapore is an island state with considerable population, industries, commerce and transport located in coastal areas at elevations less than 2 m making it vulnerable to sea-level rise. Mitigation against future inundation events requires a quantitative assessment of risk. To address this need, regional projections of changes in (i) long-term mean sea level and (ii) the frequency of extreme storm surge and wave events have been combined to explore potential changes to coastal flood risk over the 21st century. Local changes in time mean sea level were evaluated using the process-based climate model data and methods presented in the IPCC AR5. Regional surge and wave solutions extending from 1980 to 2100 were generated using 12 km resolution surge (Nucleus for European Modelling of the Ocean - NEMO) and wave (WaveWatchIII) models. Ocean simulations were forced by output from a selection of four downscaled ( 12 km resolution) atmospheric models, forced at the lateral boundaries by global climate model simulations generated for the IPCC AR5. Long-term trends in skew surge and significant wave height were then assessed using a generalised extreme value model, fit to the largest modelled events each year. An additional atmospheric solution downscaled from the ERA-Interim global reanalysis was used to force historical ocean model simulations extending from 1980-2010, enabling a quantitative assessment of model skill. Simulated historical sea surface height and significant wave height time series were compared to tide gauge data and satellite altimetry data respectively. Central estimates of the long-term mean sea level rise at Singapore by 2100 were projected to be 0.52 m(0.74 m) under the RCP 4.5(8.5) scenarios respectively. Trends in surge and significant wave height 2-year return levels were found to be statistically insignificant and/or physically very small under the more severe RCP8.5 scenario. We conclude that changes to long-term mean sea level constitute the dominant signal of change to the projected inundation risk for Singapore during the 21st century. We note that the largest recorded surge residual in the Singapore Strait of 84 cm lies between the central and upper estimates of sea level rise by 2100, highlighting the vulnerability of the region.

Projected sea level rise and changes in extreme storm surge and wave events during the 21st century in the region of Singapore

NASA Astrophysics Data System (ADS)

Cannaby, H.; Palmer, M. D.; Howard, T.; Bricheno, L.; Calvert, D.; Krijnen, J.; Wood, R.; Tinker, J.; Bunney, C.; Harle, J.; Saulter, A.; O'Neill, C.; Bellingham, C.; Lowe, J.

2015-12-01

Singapore is an island state with considerable population, industries, commerce and transport located in coastal areas at elevations less than 2 m making it vulnerable to sea-level rise. Mitigation against future inundation events requires a quantitative assessment of risk. To address this need, regional projections of changes in (i) long-term mean sea level and (ii) the frequency of extreme storm surge and wave events have been combined to explore potential changes to coastal flood risk over the 21st century. Local changes in time mean sea level were evaluated using the process-based climate model data and methods presented in the IPCC AR5. Regional surge and wave solutions extending from 1980 to 2100 were generated using ~ 12 km resolution surge (Nucleus for European Modelling of the Ocean - NEMO) and wave (WaveWatchIII) models. Ocean simulations were forced by output from a selection of four downscaled (~ 12 km resolution) atmospheric models, forced at the lateral boundaries by global climate model simulations generated for the IPCC AR5. Long-term trends in skew surge and significant wave height were then assessed using a generalised extreme value model, fit to the largest modelled events each year. An additional atmospheric solution downscaled from the ERA-Interim global reanalysis was used to force historical ocean model simulations extending from 1980-2010, enabling a quantitative assessment of model skill. Simulated historical sea surface height and significant wave height time series were compared to tide gauge data and satellite altimetry data respectively. Central estimates of the long-term mean sea level rise at Singapore by 2100 were projected to be 0.52 m (0.74 m) under the RCP 4.5 (8.5) scenarios respectively. Trends in surge and significant wave height 2 year return levels were found to be statistically insignificant and/or physically very small under the more severe RCP8.5 scenario. We conclude that changes to long-term mean sea level constitute the dominant signal of change to the projected inundation risk for Singapore during the 21st century. We note that the largest recorded surge residual in the Singapore Strait of ~ 84 cm lies between the central and upper estimates of sea level rise by 2100, highlighting the vulnerability of the region.

Test Bed Considerations for the Evaluation of EMP Protection Measures for Defense Electronics Installations.

DTIC Science & Technology

1981-05-31

number) EMP Hardening EMP Testing Electromagnetic Pulse (EMP) EMP Test Bed Facility Electromagnetic Environment Electromagnetic Susceptibility 20 ABSTRACT...very high energy electromagnetic pulse (EMP). The EMP from an exo-atmospheric :3 burst can disrupt or damage unprotected electronics over an area as...3. A., and Parker, R. L., " Electromagnetic Pulse Handbook for Missiles and Aircraft in Flight EMP Interaction 1-1," Sandia Laboratories for Air Force

Extreme storm surge and wind wave climate scenario simulations at the Venetian littoral

NASA Astrophysics Data System (ADS)

Lionello, P.; Galati, M. B.; Elvini, E.

Scenario climate projections for extreme marine storms producing storm surges and wind waves are very important for the northern flat coast of the Adriatic Sea, where the area at risk includes a unique cultural and environmental heritage, and important economic activities. This study uses a shallow water model and a spectral wave model for computing the storm surge and the wind wave field, respectively, from the sea level pressure and wind fields that have been computed by the RegCM regional climate model. Simulations cover the period 1961-1990 for the present climate (control simulations) and the period 2071-2100 for the A2 and B2 scenarios. Generalized Extreme Value analysis is used for estimating values for the 10 and 100 year return times. The adequacy of these modeling tools for a reliable estimation of the climate change signal, without needing further downscaling is shown. However, this study has mainly a methodological value, because issues such as interdecadal variability and intermodel variability cannot be addressed, since the analysis is based on single model 30-year long simulations. The control simulation looks reasonably accurate for extreme value analysis, though it overestimates/underestimates the frequency of high/low surge and wind wave events with respect to observations. Scenario simulations suggest higher frequency of intense storms for the B2 scenario, but not for the A2. Likely, these differences are not the effect of climate change, but of climate multidecadal variability. Extreme storms are stronger in future scenarios, but differences are not statistically significant. Therefore this study does not provide convincing evidence for more stormy conditions in future scenarios.

EMP1, EMP 2, and EMP3 as novel therapeutic targets in human cancer.

PubMed

Wang, Yi-Wen; Cheng, Hong-Ling; Ding, Ya-Rou; Chou, Lien-Hsuan; Chow, Nan-Haw

2017-08-01

The epithelial membrane protein genes 1, 2, and 3 (EMP1, EMP2, and EMP3) belong to the peripheral myelin protein 22-kDa (PMP22) gene family, which consists of at least seven members: PMP22, EMP1, EMP2, EMP3, PERP, brain cell membrane protein 1, and MP20. This review addresses the structural and functional features of EMPs, detailing their tissue distribution and functions in the human body, their expression pattern in a variety of tumors, and highlighting the underlying mechanisms involved in carcinogenesis. The implications in cancer biology, patient prognosis prediction, and potential application in disease therapy are discussed. For example, EMP1 was reported to be a biomarker of gefitinib resistance in lung cancer and contributes to prednisolone resistance in acute lymphoblastic leukemia patients. EMP2 functions as an oncogene in human endometrial and ovarian cancers; however, characteristics of EMP2 in urothelial cancer fulfill the criteria of a suppressor gene. Of particular interest, EMP3 overexpression in breast cancer is significantly related to strong HER-2 expression. Co-expression of HER-2 and EMP3 is the most important indicator of progression-free and metastasis-free survival for patients with urothelial carcinoma of the upper urinary tract. Altogether, discovery of pharmacological inhibitors and/or regulators of EMP protein activity could open novel strategies for enhanced therapy against EMP-mediated human diseases. Copyright © 2017 Elsevier B.V. All rights reserved.

Effect of Hydraulic Accumulator on Pressure Surge of a Hydrostatic Transmission System

NASA Astrophysics Data System (ADS)

Kumar, Ajit; Das, Jayanta; Dasgupta, Kabir; Barnwal, Manish Kumar

2018-04-01

Hydraulic power system is generally used in off-road vehicles for power transmission such as Heavy Earth Moving Machineries (HEMM). Their energy efficiency and unsubstantial failure becomes an extensive subject of analysis. Various arrangements in the system are compassed along with the utilization of some appropriate components. Application of a hydraulic accumulator is one among them. Benefits of accumulator is its multi-purpose usages like energy saving and pressure surge damping. This paper deals with the control of pressure surges in the hydraulic system and energy saving from the surges by using accumulator. For this purpose, the simulation of the hydraulic system is done in MATLAB/SimulinkR environment and an external disturbance is introduced to generate the pressure surge. The surge absorptivity of the accumulator is studied for different sizes at different pre-charged conditions of the accumulator. The discharge characteristics of different sized accumulators are also analyzed in this paper. It is observed that the ability to absorb the surge and stabilize the system is high in the smaller capacity accumulator. However the energy delivery time of larger sized accumulator is high.

Risk Assessment of Hurricane Storm Surge for Tampa Bay

NASA Astrophysics Data System (ADS)

Lin, N.; Emanuel, K.

2011-12-01

Hurricane storm surge presents a major hazard for the United States and many other coastal areas around the world. Risk assessment of current and future hurricane storm surge provides the basis for risk mitigation and related decision making. This study investigates the hurricane surge risk for Tampa Bay, located on the central west coast of Florida. Although fewer storms have made landfall in the central west Florida than in regions farther west in the Gulf of Mexico and the east coast of U.S., Tampa Bay is highly vulnerable to storm surge due to its geophysical features. It is surrounded by low-lying lands, much of which may be inundated by a storm tide of 6 m. Also, edge waves trapped on the west Florida shelf can propagate along the coastline and affect the sea level outside the area of a forced storm surge; Tampa Bay may be affected by storms traversing some distance outside the Bay. Moreover, when the propagation speed of the edge wave is close to that of a storm moving parallel to the coast, resonance may occur and the water elevation in the Bay may be greatly enhanced. Therefore, Tampa Bay is vulnerable to storms with a broad spectrum of characteristics. We apply a model-based risk assessment method to carry out the investigation. To estimate the current surge risk, we apply a statistical/deterministic hurricane model to generate a set of 1500 storms for the Tampa area, under the observed current climate (represented by 1981-2000 statistics) estimated from the NCAR/NCEP reanalysis. To study the effect of climate change, we use four climate models, CNRM-CM3, ECHAM, GFDL-CM2.0, and MIROC3.2, respectively, to drive the hurricane model to generate four sets of 1500 Tampa storms under current climate conditions (represented by 1981-2000 statistics) and another four under future climate conditions of the IPCC-AR4 A1B emission scenario (represented by 2081-2100 statistics). Then, we apply two hydrodynamic models, the Advanced Circulation (ADCIRC) model and the Sea, Lake, and Overland Surges from Hurricanes (SLOSH) model with grids of various resolutions to simulate the surges induced by the synthetic storms. The surge risk under each of the climate scenarios is depicted by a surge return level curve (exceedance probability curve). For the city of Tampa, the heights of the 100-year, 500-year, and 1000-year surges under the current climate are estimated to be 3.85, 5.66, and 6.31 m, respectively. Two of the four climate models predict that surge return periods will be significantly shortened in the future climates due to the change of storm climatology; the current 100-year surge may happen every 50 years or less, the 500-year surge every 200 years or less, and the 1000-year surge every 300 years or less. The other two climate models predict that the surge return periods will become moderately shorter or remain almost unchanged in the future climates. Extreme surges up to 12 m at Tampa appear in our simulations. Although occurring with very small probabilities, these extreme surges would have a devastating impact on the Tampa Bay area. By examining the generated synthetic surge database, we study the characteristics of the extreme storms at Tampa Bay, especially for the storms that may interact with edge waves along the Florida west coast.

Role of the Emp Pilus Subunits of Enterococcus faecium in Biofilm Formation, Adherence to Host Extracellular Matrix Components, and Experimental Infection.

PubMed

Montealegre, Maria Camila; Singh, Kavindra V; Somarajan, Sudha R; Yadav, Puja; Chang, Chungyu; Spencer, Robert; Sillanpää, Jouko; Ton-That, Hung; Murray, Barbara E

2016-05-01

Enterococcus faecium is an important cause of hospital-associated infections, including urinary tract infections (UTIs), bacteremia, and infective endocarditis. Pili have been shown to play a role in the pathogenesis of Gram-positive bacteria, including E. faecium We previously demonstrated that a nonpiliated ΔempABC::cat derivative of E. faecium TX82 was attenuated in biofilm formation and in a UTI model. Here, we studied the contributions of the individual pilus subunits EmpA, EmpB, and EmpC to pilus architecture, biofilm formation, adherence to extracellular matrix (ECM) proteins, and infection. We identified EmpA as the tip of the pili and found that deletion of empA reduced biofilm formation to the same level as deletion of the empABC operon, a phenotype that was restored by reconstituting in situ the empA gene. Deletion of empB also caused a reduction in biofilm, while EmpC was found to be dispensable. Significant reductions in adherence to fibrinogen and collagen type I were observed with deletion of empA and empB, while deletion of empC had no adherence defect. Furthermore, we showed that each deletion mutant was significantly attenuated in comparison to the isogenic parental strain, TX82, in a mixed-inoculum UTI model (P < 0.001 to 0.048), that reconstitution of empA restored virulence in the UTI model, and that deletion of empA also resulted in attenuation in an infective endocarditis model (P = 0.0088). Our results indicate that EmpA and EmpB, but not EmpC, contribute to biofilm and adherence to ECM proteins; however, all the Emp pilins are important for E. faecium to cause infection in the urinary tract. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

The Association of Baseline and Longitudinal Change in Endothelial Microparticle Count with Mortality in Chronic Kidney Disease.

PubMed

Green, Darren; Skeoch, Sarah; Alexander, M Yvonne; Kalra, Philip A; Parker, Ben

2017-01-01

Chronic kidney disease (CKD) is associated with a unique milieu of vascular pathology, and effective biomarkers of active vascular damage are lacking. A candidate biomarker is the quantification of circulating endothelial microparticles (EMPs). This study observed baseline and longitudinal EMP change (δEMP) and established the association of these with all-cause mortality and cardiovascular events in CKD. An observational study in adults with CKD (estimated glomerular filtration rate [eGFR] <60 mL/min/1.73 m2). EMPs were quantified by flow cytometry of platelet poor plasma in 2 samples 12 months apart and categorised as EMP if AnnexinV+/CD31+/CD42b- EMPs were compared between primary renal diagnoses, and correlations between EMP/δEMP and other parameters made. Adjusted hazard ratios (HRs) for time to all-cause mortality and cardiovascular events were calculated for log-transformed EMP and δEMP using a Cox proportional hazard model. There were 123 patients (age 63 ± 11 years, systolic blood pressure 135 ± 18 mm Hg, eGFR 32 ± 16 mL/min/1.73 m2). The median baseline EMP count was 144/μL (range 10-714/μL). EMPs were numerically the highest in autosomal dominant polycystic kidney disease (253 [41-610]). An increase in urine protein:creatinine ratio was associated with an increase in EMP (co-efficient 0.21, p = 0.02). The adjusted HR for all-cause mortality for EMP was 8.20 (1.67-40.2, p = 0.01) and for δEMP was 2.69 (0.04-165, p = 0.64). There was no association between EMP or δEMP and cardiovascular events. Although EMP count was a significant marker of mortality risk, longitudinal change was not. This may reflect disease-specific EMP behaviour and the limitation of EMP as a generalised biomarker in CKD. © 2017 S. Karger AG, Basel.

Effects of wave-current interaction on storm surge in the Taiwan Strait: Insights from Typhoon Morakot

NASA Astrophysics Data System (ADS)

Yu, Xiaolong; Pan, Weiran; Zheng, Xiangjing; Zhou, Shenjie; Tao, Xiaoqin

2017-08-01

The effects of wave-current interaction on storm surge are investigated by a two-dimensional wave-current coupling model through simulations of Typhoon Morakot in the Taiwan Strait. The results show that wind wave and slope of sea floor govern wave setup modulations within the nearshore surf zone. Wave setup during Morakot can contribute up to 24% of the total storm surge with a maximum value of 0.28 m. The large wave setup commonly coincides with enhanced radiation stress gradient, which is itself associated with transfer of wave momentum flux. Water levels are to leading order in modulating significant wave height inside the estuary. High water levels due to tidal change and storm surge stabilize the wind wave and decay wave breaking. Outside of the estuary, waves are mainly affected by the current-induced modification of wind energy input to the wave generation. By comparing the observed significant wave height and water level with the results from uncoupled and coupled simulations, the latter shows a better agreement with the observations. It suggests that wave-current interaction plays an important role in determining the extreme storm surge and wave height in the study area and should not be neglected in a typhoon forecast.

High glucose condition increases NADPH oxidase activity in endothelial microparticles that promote vascular inflammation.

PubMed

Jansen, Felix; Yang, Xiaoyan; Franklin, Bernardo S; Hoelscher, Marion; Schmitz, Theresa; Bedorf, Jörg; Nickenig, Georg; Werner, Nikos

2013-04-01

Diabetes is a major risk factor for cardiovascular diseases. Circulating endothelial microparticles (EMP) are increased in diabetic patients, but their potential contribution in atherogenesis is unclear. We sought to determine the role of EMP derived under high glucose conditions in the development of atherosclerosis. EMP were generated from human coronary endothelial cells (HCAEC) exposed to high glucose concentrations in order to mimic diabetic conditions. These EMP were defined as 'injured' EMP (iEMP) and their effects were compared with EMP generated from 'healthy' untreated HCAEC. iEMP injection significantly impaired endothelial function in ApoE(-/-) mice compared with EMP and vehicle treatment. Immunofluorescent experiments showed increased macrophage infiltration and adhesion protein expression in atherosclerotic lesions of iEMP-treated ApoE(-/-) mice compared with controls. To further investigate the underlying mechanism of iEMP-induced vascular inflammation, additional in vitro experiments were performed. iEMP, but not EMP, induced activation of HCAEC in a time- and dose-dependent manner and increased monocyte adhesion. Further experiments demonstrated that iEMP induced activation of HCAEC by phosphorylation of p38 into its biologically active form phospho-p38. Inhibition of p38 activation abrogated iEMP-dependent induction of adhesion proteins and monocyte adhesion on HCAEC. Moreover, we could demonstrate that iEMP show increased NADPH oxidase activity and contain significantly higher level of reactive oxygen species (ROS) than EMP. iEMP triggered ROS production in HCAEC and thereby activate p38 in an ROS-dependent manner. High glucose condition increases NADPH oxidase activity in endothelial microparticles that amplify endothelial inflammation and impair endothelial function by promoting activation of the endothelium. These findings provide new insights into the pathogenesis of diabetes-associated atherosclerosis.

Predicting EMP hazard: Lessons from studies with inhaled fibrous and non-fibrous nano- and micro-particles.

PubMed

Oberdörster, Günter; Graham, Uschi

2018-05-08

Inhalation exposure to elongated cleavage fragments occurring at mineral and rock mining and crushing operations raises important questions regarding potential health effects given their resemblance to fibers with known adverse health effects like amphibole asbestos. Thus, a major goal for establishing a toxicity profile for elongate mineral particles (EMPs) is to identify and characterize a suspected hazard and characterize a risk by examining together results of hazard and exposure assessment. This will require not only knowledge about biokinetics of inhaled EMPs but also about underlying mechanisms of effects induced by retained EMPs. In vitro toxicity assays with predictive power for in vivo effects have been established as useful screening tools for toxicological characterization of particulate materials including EMPs. Important determinants of physiological/toxicological mechanisms are physico-chemical and functional properties of inhaled particulate materials. Of the physico-chemical (intrinsic) properties, size, shape and surface characteristics are well known to affect toxicological responses; functional properties include (i) solubility/dissolution rate in physiological fluid simulants in vitro and following inhalation in vivo; (ii) ROS-inducing capacity in vitro and in vivo determined as specific particle surface reactivity; (iii) bioprocessing in vivo. A key parameter for all is the dose and duration of exposure, requiring to establish exposure-dose-response relationships. Examples of studies with fibrous and non-fibrous particles are discussed to illustrate the relevancy of evaluating extrinsic and intrinsic particle properties for predicting in vivo responses of new particulate materials. This will allow hazard and risk ranking/grouping based on a comparison to toxicologically well-characterized positive and negative benchmarks. Future efforts should be directed at developing and validating new approaches using in vitro (non-animal) studies for establishing a complete risk assessment for EMPs. Further comparative in-depth analyses with analytical and ultra-high resolution technology examining bioprocessing events at target organ sites have proven highly successful to identify biotransformations in target cells at near atomic level. In the case of EMPs, such analyses can be essential to separate benign from harmful ones. Copyright © 2018. Published by Elsevier Inc.

Increasing Flood Risk due to Run-off Outflow near Estuarine City during Storm Event

NASA Astrophysics Data System (ADS)

Son, S.; Lee, C.; Do, K.; Jung, T.

2017-12-01

Tropical cyclone easily causes inundation damage to low-lying coastal area and the damage may be amplified due to tide motion, sea-level rise, riverine discharges. Specifically, typhoons are accompanied by intensive rainfall, which will of course raise the river water level and thus enhance the flooding damages. If the tidal cycle coincides the high water, flooding will be even aggravated. In the present study, we simulated storm surge motions at the coastal area considering combined effects of tidal and river discharge with aim to improve the accuracy of flooding prediction. The quasi 3-dimension ocean circulation model, Delf3D was used which solves the unsteady shallow water equation in the 2D and 3D. Since Delft3D is much applicable to accommodate the indirect flooding factors such as riverine discharge and short waves, outer-coupled modeling system was established to account for combined tide-surge-riverine discharge effects. In such integrated system, 11 tidal constituents were input as open boundary condition using TPXO 7.2 model, while the water level per unit time was preliminary calculated by HEC-HMS model and input as the upstream boundary conditions for river inside the domain. Typhoon MAEMI which attacked Masan city located at southern coast of South Korea and caused severe inundation damages in 2003 was selected for the study event. Basic information for typhoon such as path, wind speed, atmospheric pressure every 3 hours was provided by the Korea Meteorological Agency and was adopted. The simulation was implemented with tide and storm surge boundary conditions focusing on the target area, Masan, while the additional consideration on the discharge of the river inside the domain was also made. Simulated water level at the fixed location was compared to the observation for its verification and the extent of inundation areas of Masan were compared between observed and calculated. The marginal contribution of riverine discharge on the flooding area(or depth) was assessed by comparing tide-surge with tide-surge-riverine discharge simulations. Finally, the importance of the specific consideration on the riverine discharge during storm surge modeling can be addressed.

Forecasting of Storm-Surge Floods Using ADCIRC and Optimized DEMs

NASA Technical Reports Server (NTRS)

Valenti, Elizabeth; Fitzpatrick, Patrick

2006-01-01

Increasing the accuracy of storm-surge flood forecasts is essential for improving preparedness for hurricanes and other severe storms and, in particular, for optimizing evacuation scenarios. An interactive database, developed by WorldWinds, Inc., contains atlases of storm-surge flood levels for the Louisiana/Mississippi gulf coast region. These atlases were developed to improve forecasting of flooding along the coastline and estuaries and in adjacent inland areas. Storm-surge heights depend on a complex interaction of several factors, including: storm size, central minimum pressure, forward speed of motion, bottom topography near the point of landfall, astronomical tides, and, most importantly, maximum wind speed. The information in the atlases was generated in over 100 computational simulations, partly by use of a parallel-processing version of the ADvanced CIRCulation (ADCIRC) model. ADCIRC is a nonlinear computational model of hydrodynamics, developed by the U.S. Army Corps of Engineers and the US Navy, as a family of two- and three-dimensional finite-element-based codes. It affords a capability for simulating tidal circulation and storm-surge propagation over very large computational domains, while simultaneously providing high-resolution output in areas of complex shoreline and bathymetry. The ADCIRC finite-element grid for this project covered the Gulf of Mexico and contiguous basins, extending into the deep Atlantic Ocean with progressively higher resolution approaching the study area. The advantage of using ADCIRC over other storm-surge models, such as SLOSH, is that input conditions can include all or part of wind stress, tides, wave stress, and river discharge, which serve to make the model output more accurate. To keep the computational load manageable, this work was conducted using only the wind stress, calculated by using historical data from Hurricane Camille, as the input condition for the model. Hurricane storm-surge simulations were performed on an eight-node Linux computer cluster. Each node contained dual 2-GHz processors, 2GB of memory, and a 40GB hard drive. The digital elevation model (DEM) for this region was specified using a combination of Navy data (over water), NOAA data (for the coastline), and optimized Interferometric Synthetic Aperture Radar data (over land). This high-resolution topographical data of the Mississippi coastal region provided the ADCIRC model with improved input with which to calculate improved storm-surge forecasts.

Measurement of electromagnetic pulses generated during interactions of high power lasers with solid targets

NASA Astrophysics Data System (ADS)

De Marco, M.; Krása, J.; Cikhardt, J.; Pfeifer, M.; Krouský, E.; Margarone, D.; Ahmed, H.; Borghesi, M.; Kar, S.; Giuffrida, L.; Vrana, R.; Velyhan, A.; Limpouch, J.; Korn, G.; Weber, S.; Velardi, L.; Delle Side, D.; Nassisi, V.; Ullschmied, J.

2016-06-01

A target irradiated with a high power laser pulse, blows off a large amount of charge and as a consequence the target itself becomes a generator of electromagnetic pulses (EMP) owing to high return current flowing to the ground through the target holder. The first measurement of the magnetic field induced by the neutralizing current reaching a value of a few kA was performed with the use of an inductive target probe at the PALS Laser Facility (Cikhardt et al. Rev. Sci. Instrum. 85 (2014) 103507). A full description of EMP generation should contain information on the spatial distribution and temporal variation of the electromagnetic field inside and outside of the interaction chamber. For this reason, we consider the interaction chamber as a resonant cavity in which different modes of EMP oscillate for hundreds of nanoseconds, until the EMP is transmitted outside through the glass windows and EM waves are attenuated. Since the experimental determination of the electromagnetic field distribution is limited by the number of employed antennas, a mapping of the electromagnetic field has to be integrated with numerical simulations. Thus, this work reports on a detailed numerical mapping of the electromagnetic field inside the interaction chamber at the PALS Laser Facility (covering a frequency spectrum from 100 MHz to 3 GHz) using the commercial code COMSOL Multiphysics 5.2. Moreover we carried out a comparison of the EMP generated in the parallelepiped-like interaction chamber used in the Vulcan Petawatt Laser Facility at the Rutherford Appleton Laboratory, against that produced in the spherical interaction chamber of PALS.

High glucose increases the formation and pro-oxidative activity of endothelial microparticles.

PubMed

Burger, Dylan; Turner, Maddison; Xiao, Fengxia; Munkonda, Mercedes N; Akbari, Shareef; Burns, Kevin D

2017-09-01

Individuals with diabetes exhibit increases in circulating endothelial microparticles (eMPs, also referred to as endothelial microvesicles), which are associated with endothelial dysfunction and a heightened risk of cardiovascular complications. We have shown that eMPs are markers and mediators of vascular injury although their role in diabetes is unclear. We hypothesised that the composition and biological activity of eMPs are altered in response to high glucose exposure. We assessed the effects of high glucose on eMP formation, composition and signalling in cultured HUVECs. eMPs were isolated from the media of HUVECs cultured under conditions of normal glucose (eMP NG ), high glucose (eMP HG ) or osmotic control of L-glucose (eMP LG ). eMP size, concentration and surface charge were assessed by nanoparticle tracking analysis and flow cytometry. eMP protein composition was assessed by liquid chromatography-tandem mass spectrometry, and eMP-mediated effects on coagulation, reactive oxygen species (ROS) production and vessel function were assessed. Exposure of HUVECs to high glucose for 24 h caused a threefold increase in eMP formation, increased mean particle size (269 ± 18 nm vs 226 ± 11 nm) and decreased surface charge. Compared with eMP NG or eMP LG , eMP HG possessed approximately threefold greater pro-coagulant activity, stimulated HUVEC ROS production to a greater extent (~250% of eMP NG ) and were more potent inhibitors of endothelial-dependent relaxation. Proteomic analysis of eMPs identified 1212 independent proteins of which 68 were exclusively found in eMP HG . Gene ontology analysis revealed that eMP HG -exclusive proteins were associated with signalling pathways related to blood coagulation, cell signalling and immune cell activation. Our results indicate that elevated glucose is a potent stimulus for eMP formation that also alters their molecular composition leading to increased bioactivity. Such effects may contribute to progressive endothelial injury and subsequent cardiovascular complications in diabetes.

[Skill retention using extraglottic airways in out-of-hospital emergencies: efficacy and long-term results of simulator-based medical education : A prospective follow-up study].

PubMed

Mann, V; Limberg, F; Mann, S T W; Little, S; Müller, M; Sander, M; Röhrig, R

2018-04-11

For emergency medicine personnel (EMP), there is little evidence concerning the adequate timing for refresher courses to maintain routine in the application of extraglottic airways. The aim of this study was to evaluate the efficacy and long-term results of a simulator-based education concept teaching the basic airway management skills with extraglottic airways for EMP and also to draw conclusions concerning the adequate time interval for refresher courses. By use of an explorative, prospective simulator-study with nonphysician EMP, airway management skills using the Larynxmaske Supreme® (LMA‑S) after an introduction lecture were examined. The application of an endotracheal tube (ETT) served as control. Time for preparation of the airway devices, insertion success, and resulting apnea time were assessed immediately after the first introduction lecture (t1) and unannounced 9-12 months thereafter (t2). Comparison of the times for preparation of the LMA‑S at t1 and t2 demonstrated similar results. After the introduction lecture, all paramedics were able to insert the LMA‑S successfully after maximal 2 attempts; 9-12 months later success rates with the LMA‑S were unchanged. Apnea time during airway management was shorter with the LMA‑S compared to the ETT (p < 0.01). Times needed for preparation of the airway devices were similar. The results of this simulator study indicate that a standardized introduction lecture is appropriate to ensure long-lasting procedural skills up to 12 months, so that subsequent refresher courses in basic airway management with the LMA‑S once a year may be adequate. A simulator-based education in basic airway management skills with extraglottic airways is recommended for facilitation of further clinical education according to the current guidelines.

Assessing extreme sea levels due to tropical cyclones in the Atlantic basin

NASA Astrophysics Data System (ADS)

Muis, Sanne; Lin, Ning; Verlaan, Martin; Winsemius, Hessel; Vatvani, Deepak; Ward, Philip; Aerts, Jeroen

2017-04-01

Tropical cyclones (TCs), including hurricanes and typhoons, are characterised by high wind speeds and low pressure and cause dangerous storm surges in coastal areas. Over the last 50 years, storm surge incidents in the Atlantic accounted for more than 1,000 deaths in the United Stated. Recent flooding disasters, such as Hurricane Katrina in New Orleans in 2005 and, Hurricane Sandy in New York in 2012, exemplify the significant TC surge risk in the United States. In this contribution, we build on Muis et al. (2016), and present a new modelling framework to simulate TC storm surges and estimate their probabilities for the Atlantic basin. In our framework we simulate the surge levels by forcing the Global Tide and Surge Model (GTSM) with wind and pressure fields from TC events. To test the method, we apply it to historical storms that occurred between 1988 and 2015 in the Atlantic Basin. We obtain high-resolution meteorological forcing by applying a parametric hurricane model (Holland 1980; Lin and Chavas 2012) to the TC extended track data set (Demuth et al. 2006; updated), which describes the position, intensity and size of the historical TCs. Preliminary results show that this framework is capable of accurately reproducing the main surge characteristics during past events, including Sandy and Katrina. While the resolution of GTSM is limited for local areas with a complex bathymetry, the overall performance of the model is satisfactory for the basin-scale application. For an accurate assessment of risk to coastal flooding in the Atlantic basin it is essential to provide reliable estimates of surge probabilities. However, the length of observed TC tracks is too short to accurately estimate the probabilities of extreme TC events. So next steps are to statistically extend the observed record to many thousands of years (e.g., Emanuel et al. 2006), in order to force GTSM with a large number of synthetic storms. Based on these synthetic simulations, we would be able to provide reliable probabilities of surge levels for the entire Atlantic basin. References Demuth, J., DeMaria, M., and Knaff, J.A. (2006). Improvement of advanced microwave sounder unit tropical cyclone intensity and size estimation algorithms. Journal of Applied Meteorology., 45, pp. 1573-1581. Emanuel, K., Ravela, S., Vivant, E. and Risi, C. (2006). A statistical deterministic approach to hurricane risk assessment. Bulletin of the American Meteorological Society, 87(3), pp.299-314. Holland, G.J. (1980). An analytic model of the wind and pressure profiles in hurricanes. Monthly Weather Review, 108(8), pp.1212-1218. Lin, N. and D. Chavas (2012). On hurricane parametric wind and applications in storm surge modeling. Journal of Geophysical Research - Atmospheres. 117. doi:10.1029/2011jd017126. Muis, S., Verlaan, M., Winsemius, H. C., Aerts, J. C. J. H., & Ward, P. J. (2016). A global reanalysis of storm surge and extreme sea levels. Nature Communications, 7(7:11969), 1-11.

Electric Field Simulation of Surge Capacitors with Typical Defects

NASA Astrophysics Data System (ADS)

Zhang, Chenmeng; Mao, Yuxiang; Xie, Shijun; Zhang, Yu

2018-03-01

The electric field of power capacitors with different typical defects in DC working condition and impulse oscillation working condition is studied in this paper. According to the type and location of defects and considering the influence of space charge, two-dimensional models of surge capacitors with different typical defects are simulated based on ANSYS. The distribution of the electric field inside the capacitor is analyzed, and the concentration of electric field and its influence on the insulation performance are obtained. The results show that the type of defects, the location of defects and the space charge all affect the electric field distribution inside the capacitor in varying degrees. Especially the electric field distortion in the local area such as sharp corners and burrs is relatively larger, which increases the probability of partial discharge inside the surge capacitor.

Mass ejections. [during solar flares

NASA Technical Reports Server (NTRS)

Rust, D. M.; Hildner, E.; Hansen, R. T.; Dryer, M.; Mcclymont, A. N.; Mckenna-Lawlor, S. M. P.; Mclean, D. J.; Schmahl, E. J.; Steinolfson, R. S.; Tandberg-Hanssen, E.

1980-01-01

Observations and model simulations of solar mass ejection phenomena are examined in an investigation of flare processes. Consideration is given to Skylab and other observations of flare-associated sprays, eruptive prominences, surges and coronal transients, and to MHD, gas dynamic and magnetic loop models developed to account for them. Magnetic forces are found to confine spray material, which originates in preexisting active-region filaments, within steadily expanding loops, while surges follow unmoving, preexisting magnetic field lines. Simulations of effects of a sudden pressure pulse at the bottom of the corona are found to exhibit many characteristics of coronal transients associated with flares, and impulsive heating low in the chromosphere is found to be able to account for surges. The importance of the magnetic field as the ultimate source of energy which drives eruptive phenomena as well as flares is pointed out.

Application of short-data methods on extreme surge levels

NASA Astrophysics Data System (ADS)

Feng, X.

2014-12-01

Tropical cyclone-induced storm surges are among the most destructive natural hazards that impact the United States. Unfortunately for academic research, the available time series for extreme surge analysis are very short. The limited data introduces uncertainty and affects the accuracy of statistical analyses of extreme surge levels. This study deals with techniques applicable to data sets less than 20 years, including simulation modelling and methods based on the parameters of the parent distribution. The verified water levels from water gauges spread along the Southwest and Southeast Florida Coast, as well as the Florida Keys, are used in this study. Methods to calculate extreme storm surges are described and reviewed, including 'classical' methods based on the generalized extreme value (GEV) distribution and the generalized Pareto distribution (GPD), and approaches designed specifically to deal with short data sets. Incorporating global-warming influence, the statistical analysis reveals enhanced extreme surge magnitudes and frequencies during warm years, while reduced levels of extreme surge activity are observed in the same study domain during cold years. Furthermore, a non-stationary GEV distribution is applied to predict the extreme surge levels with warming sea surface temperatures. The non-stationary GEV distribution indicates that with 1 Celsius degree warming in sea surface temperature from the baseline climate, the 100-year return surge level in Southwest and Southeast Florida will increase by up to 40 centimeters. The considered statistical approaches for extreme surge estimation based on short data sets will be valuable to coastal stakeholders, including urban planners, emergency managers, and the hurricane and storm surge forecasting and warning system.

Impacts of Storm Surge Mitigation Strategies on Aboveground Storage Tank Chemical Spill Transport

NASA Astrophysics Data System (ADS)

Do, C.; Bass, B. J.; Bernier, C.; Samii, A.; Dawson, C.; Bedient, P. B.

2017-12-01

The Houston Ship Channel (HSC), located in the hurricane-prone Houston-Galveston region of the upper Texas Coast, is one of the busiest waterways in the United States and is home to one of the largest petrochemical complexes in the world. Due to the proximity of the HSC to Galveston Bay and the Gulf of Mexico, chemical spills resulting from storm surge damage to aboveground storage tanks (ASTs) pose serious threats to the environment, residential communities, and national/international markets whose activities in the HSC generate billions of dollars annually. In an effort to develop a comprehensive storm surge mitigation strategy for Galveston Bay and its constituents, Rice University's Severe Storm Prediction, Education, and Evacuation from Disasters Center proposed two structural storm surge mitigation concepts, the Mid Bay Structure (MBS) and the Lower Bay Structure (LBS) as components of the Houston-Galveston Area Protection System (H-GAPS) project. The MBS consists of levees along the HSC and a navigational gate across the channel, and the LBS consists of a navigation gate and environmental gates across Bolivar Road. The impacts of these two barrier systems on the fate of AST chemical spills in the HSC have previously been unknown. This study applies the coupled 2D SWAN+ADCIRC model to simulate hurricane storm surge circulation within the Gulf of Mexico and Galveston Bay due to a synthetic storm which results in approximately 250-year surge levels in Galveston Bay. The SWAN+ADCIRC model is run using high-resolution computational meshes that incorporate the MBS and LBS scenarios, separately. The resulting wind and water velocities are then fed into a Lagrangian particle transport model to simulate the spill trajectories of the ASTs most likely to fail during the 250-year proxy storm. Results from this study illustrate how each storm surge mitigation strategy impacts the transport of chemical spills (modeled as Lagrangian particles) during storm surge as compared to the base case with only currently existing mitigation features in place. This study is part of a larger effort to evaluate the costs and benefits of several storm surge mitigation configurations proposed by the H-GAPS project.

Endothelial microparticles-mediated transfer of microRNA-19b promotes atherosclerosis via activating perivascular adipose tissue inflammation in apoE-/- mice.

PubMed

Li, Changlong; Li, Sufang; Zhang, Feng; Wu, Manyan; Liang, Huizhu; Song, Junxian; Lee, Chongyou; Chen, Hong

2018-01-08

Microparticles(MPs) are the major carriers of circulating microRNAs. Our previous study has shown that microRNA (miR)-19b in endothelial cell-derived microparticles (EMPs) is significantly increased in patients with unstable angina. However, little is known about the relationship between miR-19b in EMPs and the progression of atherosclerosis. The aim of the present study was to define the role and potential mechanism of miR-19b incorporated in EMPs in the development of atherosclerosis. Western-diet-fed apoE -/- mice were injected with phosphate buffered solution(PBS), EMP carrying microRNA control(EMP control ) or miR-19b mimic (EMP miR19b ) intravenously. Systemic treatment with EMP miR19b significantly accelerated carotid artery atherosclerosis progression by increasing lipid, macrophages and smooth muscle cells and decreasing collagen content in atherosclerotic plaque. Fluorescence-labelled EMP miR19b injection proved that miR-19b could be transported into perivascular adipose tissue(PVAT) by EMPs. EMP miR19b treatment also promoted inflammatory cytokines secretion and macrophages infiltration in PVAT. In further experiment, apoE -/- mice were divided into 3 groups: EMP control PVAT(+), EMP miR19b PVAT(+) and EMP miR19b PVAT(-), based on removing or keeping pericarotid adipose tissue and injected with EMP control or EMP miR19b . Loss of PVAT attenuated EMP miR19b -mediated effects on increasing carotid atherosclerosis formation and inflammatory cytokines level in plaque. EMP miR19b inhibited suppressor of cytokine signaling 3 (SOCS3) expression in PVAT. Our findings demonstrate that miR-19b in EMPs exaggerates atherosclerosis progression by augmenting PVAT-specific inflammation proceeded by downregulating SOCS3 expression. Copyright © 2017 Elsevier Inc. All rights reserved.

Effect of bottle height and aspiration rate on postocclusion surge in Infiniti and Millennium peristaltic phacoemulsification machines.

PubMed

Ward, Matthew S; Georgescu, Dan; Olson, Randall J

2008-08-01

To assess how flow and bottle height affect postocclusion surge in the Infiniti (Alcon, Inc.) and Millennium (Bausch & Lomb) peristaltic machines. John A. Moran Eye Center Clinical Laboratories, University of Utah, Salt Lake City, Utah. Postocclusion anterior chamber depth changes were measured in human eye-bank eyes using A-scan. Surge was simulated by clamping the aspiration tubing and releasing it at maximum vacuum. In both machines, surge was measured (1) with aspiration held constant at 12 mL/min and bottle heights at 60, 120, and 180 cm and (2) with bottle height held constant at 60 cm and aspiration rates at 12, 24, and 36 mL/min. Surge decreased approximately 40% with each 60 cm increase in bottle height in the Infiniti. It was constant at all bottle heights in the Millennium. At 12 and 24 mL/min aspiration rates, surge in the Millennium was less than half that in the Infiniti (P<.001). Postocclusion surge decreased linearly with increasing bottle height in the Infiniti system and was relatively constant with increasing bottle height in the Millennium system. The Millennium may offer a more stable phacoemulsification platform with respect to surge at a higher aspiration rate.

Endothelial microparticle uptake in target cells is annexin I/phosphatidylserine receptor dependent and prevents apoptosis.

PubMed

Jansen, Felix; Yang, Xiaoyan; Hoyer, Friedrich Felix; Paul, Kathrin; Heiermann, Nadine; Becher, Marc Ulrich; Abu Hussein, Nebal; Kebschull, Moritz; Bedorf, Jörg; Franklin, Bernardo S; Latz, Eicke; Nickenig, Georg; Werner, Nikos

2012-08-01

Endothelial microparticles (EMP) are released from activated or apoptotic cells, but their effect on target cells and the exact way of incorporation are largely unknown. We sought to determine the uptake mechanism and the biological effect of EMP on endothelial and endothelial-regenerating cells. EMP were generated from starved endothelial cells and isolated by ultracentrifugation. Caspase 3 activity assay and terminal deoxynucleotidyl transferase dUTP nick end labeling assay showed that EMP protect target endothelial cells against apoptosis in a dose-dependent manner. Proteomic analysis was performed to identify molecules contained in EMP, which might be involved in EMP uptake. Expression of annexin I in EMP was found and confirmed by Western blot, whereas the corresponding receptor phosphatidylserine receptor was present on endothelial target cells. Silencing either annexin I on EMP or phosphatidylserine receptor on target cells using small interfering RNA showed that the uptake of EMP by human coronary artery endothelial cells is annexin I/phosphatidylserine receptor dependent. Annexin I-downregulated EMP abrogated the EMP-mediated protection against apoptosis of endothelial target cells. p38 activation was found to mediate camptothecin-induced apoptosis. Finally, human coronary artery endothelial cells pretreated with EMP inhibited camptothecin-induced p38 activation. EMP are incorporated by endothelial cells in an annexin I/phosphatidylserine receptor-dependent manner and protect target cells against apoptosis. Inhibition of p38 activity is involved in EMP-mediated protection against apoptosis.

Developpement dune methode de simulation de pompage au sein d'un compresseur multi-etage

NASA Astrophysics Data System (ADS)

Dumas, Martial

Surge is an unsteady phenomenon which appears when a compressor operates at a mass flow that is too low relative to its design point. This aerodynamic instability is characterized by large oscillations in pressure and mass flow, resulting in a sudden drop in power delivered by a gas turbine engine and possibly important damage to engine components. The methodology developed in this thesis allows for the simulations of the flow behavior inside a multi-stage compressor during surge and, by extension, predict at the design phase the time variation of aerodynamic forces on the blades and of the pressure and temperature at bleed locations inside the compressors for turbine cooling. While the compressor is the component of interest and the trigger for surge, the flow behavior during this event is also dependent on other engine components (combustion chamber, turbine, ducts). However, the simulation of the entire gas turbine engine cannot be carried out in a practical manner with existing computational technologies. The approach taken consists of coupling 3-D RANS CFD simulations of the compressor with 1-D equations modeling the behavior of the other components applied as dynamic boundary conditions. The method was put into practice in a commercial RANS CFD code (ANSYS CFX) whose integrated options facilitated the implementation of the 1-D equations into the dynamic boundary conditions of the computational domain. In addition, in order to limit computational time, only one blade passage was simulated per blade row to capture surge which is essentially a one-dimensional phenomenon. This methodology was applied to several compressor geometries with distinct features. Simulations on a low-speed (incompressible) three-stage axial compressor allowed for a validation with experimental data, which showed that the pressure and mass flow oscillations are captured well. This comparison also highlighted the strong dependence of the oscillation frequency on the volume of the downstream plenum (combustion chamber). The simulations of the second compressor demonstrated the adaptability of the approach to a multi-stage compressor with an axial-centrifugal configuration. Finally, application of the method to a transonic compressor geometry from Pratt & Whitney Canada demonstrated the tool on a mixed flow-centrifugal compressor configuration operating in a highly compressible regime. These last simulations highlighted certain limitations of the tool, namely the numerical robustness associated with the use of multiple stator/rotor interfaces in a high-speed compressor with high rates of change of mass flow, and the computational time required to a simulate several surge cycles.

The Climatology of Extreme Surge-Producing Extratropical Cyclones in Observations and Models

NASA Astrophysics Data System (ADS)

Catalano, A. J.; Broccoli, A. J.; Kapnick, S. B.

2016-12-01

Extreme coastal storms devastate heavily populated areas around the world by producing powerful winds that can create a large storm surge. Both tropical and extratropical cyclones (ETCs) occur over the northwestern Atlantic Ocean, and the risks associated with ETCs can be just as severe as those associated with tropical storms (e.g. high winds, storm surge). At The Battery in New York City, 17 of the 20 largest storm surge events were a consequence of extratropical cyclones (ETCs), which are more prevalent than tropical cyclones in the northeast region of the United States. Therefore, we analyze the climatology of ETCs that are capable of producing a large storm surge along the northeastern coast of the United States. For a historical analysis, water level data was collected from National Oceanic and Atmospheric Administration (NOAA) tide gauges at three separate locations (Sewell's Pt., VA, The Battery, NY, and Boston, MA). We perform a k-means cluster analysis of sea level pressure from the ECMWF 20th Century Reanalysis dataset (ERA-20c) to explore the natural sets of observed storms with similar characteristics. We then composite cluster results with features of atmospheric circulation to observe the influence of interannual and multidecadal variability such as the North Atlantic Oscillation. Since observational records contain a small number of well-documented ETCs, the capability of a high-resolution coupled climate model to realistically simulate such extreme coastal storms will also be assessed. Global climate models provide a means of simulating a much larger sample of extreme events, allowing for better resolution of the tail of the distribution. We employ a tracking algorithm to identify ETCs in a multi-century simulation under present-day conditions. Quantitative comparisons of cyclolysis, cyclogenesis, and cyclone densities of simulated ETCs and storms from recent history (using reanalysis products) are conducted.

Forecasting of Storm Surge Floods Using ADCIRC and Optimized DEMs

NASA Technical Reports Server (NTRS)

Valenti, Elizabeth; Fitzpatrick, Patrick

2005-01-01

Increasing the accuracy of storm surge flood forecasts is essential for improving preparedness for hurricanes and other severe storms and, in particular, for optimizing evacuation scenarios. An interactive database, developed by WorldWinds, Inc., contains atlases of storm surge flood levels for the Louisiana/Mississippi gulf coast region. These atlases were developed to improve forecasting of flooding along the coastline and estuaries and in adjacent inland areas. Storm surge heights depend on a complex interaction of several factors, including: storm size, central minimum pressure, forward speed of motion, bottom topography near the point of landfall, astronomical tides, and most importantly, maximum wind speed. The information in the atlases was generated in over 100 computational simulations, partly by use of a parallel-processing version of the ADvanced CIRCulation (ADCIRC) model. ADCIRC is a nonlinear computational model of hydrodynamics, developed by the U.S. Army Corps of Engineers and the US Navy, as a family of two- and three-dimensional finite element based codes. It affords a capability for simulating tidal circulation and storm surge propagation over very large computational domains, while simultaneously providing high-resolution output in areas of complex shoreline and bathymetry. The ADCIRC finite-element grid for this project covered the Gulf of Mexico and contiguous basins, extending into the deep Atlantic Ocean with progressively higher resolution approaching the study area. The advantage of using ADCIRC over other storm surge models, such as SLOSH, is that input conditions can include all or part of wind stress, tides, wave stress, and river discharge, which serve to make the model output more accurate.

Simulated storm surge effects on freshwater coastal wetland soil porewater salinity and extractable ammonium levels: Implications for marsh recovery after storm surge

NASA Astrophysics Data System (ADS)

McKee, M.; White, J. R.; Putnam-Duhon, L. A.

2016-11-01

Coastal wetland systems experience both short-term changes in salinity, such as those caused by wind-driven tides and storm surge, and long-term shifts caused by sea level rise. Salinity increases associated with storm surge are known to have significant effects on soil porewater chemistry, but there is little research on the effect of flooding length on salt penetration depth into coastal marsh soils. A simulated storm surge was imposed on intact soil columns collected from a non-vegetated mudflat and a vegetated marsh site in the Wax Lake Delta, LA. Triplicate intact cores were continuously exposed to a 35 salinity water column (practical salinity scale) for 1, 2, and 4 weeks and destructively sampled in order to measure porewater salinity and extractable NH4sbnd N at two cm depth intervals. Salinity was significantly higher in the top 8 cm for both the marsh and mudflat cores after one week of flooding. After four weeks of flooding, salinity was significantly higher in marsh and mudflat cores compared to the control (no salinity) cores throughout the profile for both sites. Extractable ammonium levels increased significantly in the marsh cores throughout the experiment, but there was only a marginally (p < 0.1) significant increase seen in the mudflat cores. Results indicate that porewater salinity levels can become significantly elevated within a coastal marsh soil in just one week. This vertical intrusion of salt can potentially negatively impact macrophytes and associated microbial communities for significantly longer term post-storm surge.

Strengthening the resiliency of the coastal transportation system through integrated simulation of storm surge, inundation, and non-recurrent congestion in Northeast Florida.

DOT National Transportation Integrated Search

2013-05-01

In this study, the MTEVA (Developed as part of CMS #2009-010) has been advanced to apply storm surge and evacuation models to the greater Jacksonville area of Northeast Florida. Heuristic and time dynamic algorithms have been enhanced to work with th...

Impact of topography on groundwater salinization due to ocean surge inundation

NASA Astrophysics Data System (ADS)

Yu, Xuan; Yang, Jie; Graf, Thomas; Koneshloo, Mohammad; O'Neal, Michael A.; Michael, Holly A.

2016-08-01

Sea-level rise and increases in the frequency and intensity of ocean surges caused by climate change are likely to exacerbate adverse effects on low-lying coastal areas. The landward flow of water during ocean surges introduces salt to surficial coastal aquifers and threatens groundwater resources. Coastal topographic features (e.g., ponds, dunes, barrier islands, and channels) likely have a strong impact on overwash and salinization processes, but are generally highly simplified in modeling studies. To understand topographic impacts on groundwater salinization, we modeled a theoretical overwash event and variable-density groundwater flow and salt transport in 3-D using the fully coupled surface and subsurface numerical simulator, HydroGeoSphere. The model simulates the coastal aquifer as an integrated system considering overland flow, coupled surface and subsurface exchange, variably saturated flow, and variable-density groundwater flow. To represent various coastal landscape types, we simulated both synthetic fields and real-world coastal topography from Delaware, USA. The groundwater salinization assessment suggested that the topographic connectivity promoting overland flow controls the volume of aquifer that is salinized. In contrast, the amount of water that can be stored in surface depressions determines the amount of seawater that infiltrates the subsurface and the time for seawater to flush from the aquifer. Our study suggests that topography has a significant impact on groundwater salinization due to ocean surge overwash, with important implications for coastal land management and groundwater vulnerability assessment.

Projected sea level rise and changes in extreme storm surge and wave events during the 21st century in the region of Singapore

NASA Astrophysics Data System (ADS)

Cannaby, Heather; Palmer, Matthew D.; Howard, Tom; Bricheno, Lucy; Calvert, Daley; Krijnen, Justin; Wood, Richard; Tinker, Jonathan; Bunney, Chris; Harle, James; Saulter, Andrew; O'Neill, Clare; Bellingham, Clare; Lowe, Jason

2016-05-01

Singapore is an island state with considerable population, industries, commerce and transport located in coastal areas at elevations less than 2 m making it vulnerable to sea level rise. Mitigation against future inundation events requires a quantitative assessment of risk. To address this need, regional projections of changes in (i) long-term mean sea level and (ii) the frequency of extreme storm surge and wave events have been combined to explore potential changes to coastal flood risk over the 21st century. Local changes in time-mean sea level were evaluated using the process-based climate model data and methods presented in the United Nations Intergovernmental Panel on Climate Change Fifth Assessment Report (IPCC AR5). Regional surge and wave solutions extending from 1980 to 2100 were generated using ˜ 12 km resolution surge (Nucleus for European Modelling of the Ocean - NEMO) and wave (WaveWatchIII) models. Ocean simulations were forced by output from a selection of four downscaled ( ˜ 12 km resolution) atmospheric models, forced at the lateral boundaries by global climate model simulations generated for the IPCC AR5. Long-term trends in skew surge and significant wave height were then assessed using a generalised extreme value model, fit to the largest modelled events each year. An additional atmospheric solution downscaled from the ERA-Interim global reanalysis was used to force historical ocean model simulations extending from 1980 to 2010, enabling a quantitative assessment of model skill. Simulated historical sea-surface height and significant wave height time series were compared to tide gauge data and satellite altimetry data, respectively. Central estimates of the long-term mean sea level rise at Singapore by 2100 were projected to be 0.52 m (0.74 m) under the Representative Concentration Pathway (RCP)4.5 (8.5) scenarios. Trends in surge and significant wave height 2-year return levels were found to be statistically insignificant and/or physically very small under the more severe RCP8.5 scenario. We conclude that changes to long-term mean sea level constitute the dominant signal of change to the projected inundation risk for Singapore during the 21st century. We note that the largest recorded surge residual in the Singapore Strait of ˜ 84 cm lies between the central and upper estimates of sea level rise by 2100, highlighting the vulnerability of the region.

Erythro-myeloid progenitors can differentiate from endothelial cells and modulate embryonic vascular remodeling

PubMed Central

Kasaai, Bahar; Caolo, Vincenza; Peacock, Hanna M.; Lehoux, Stephanie; Gomez-Perdiguero, Elisa; Luttun, Aernout; Jones, Elizabeth A. V.

2017-01-01

Erythro-myeloid progenitors (EMPs) were recently described to arise from the yolk sac endothelium, just prior to vascular remodeling, and are the source of adult/post-natal tissue resident macrophages. Questions remain, however, concerning whether EMPs differentiate directly from the endothelium or merely pass through. We provide the first evidence in vivo that EMPs can emerge directly from endothelial cells (ECs) and demonstrate a role for these cells in vascular development. We find that EMPs express most EC markers but late EMPs and EMP-derived cells do not take up acetylated low-density lipoprotein (AcLDL), as ECs do. When the endothelium is labelled with AcLDL before EMPs differentiate, EMPs and EMP-derived cells arise that are AcLDL+. If AcLDL is injected after the onset of EMP differentiation, however, the majority of EMP-derived cells are not double labelled. We find that cell division precedes entry of EMPs into circulation, and that blood flow facilitates the transition of EMPs from the endothelium into circulation in a nitric oxide-dependent manner. In gain-of-function studies, we inject the CSF1-Fc ligand in embryos and found that this increases the number of CSF1R+ cells, which localize to the venous plexus and significantly disrupt venous remodeling. This is the first study to definitively establish that EMPs arise from the endothelium in vivo and show a role for early myeloid cells in vascular development. PMID:28272478

Are inundation limit and maximum extent of sand useful for differentiating tsunamis and storms? An example from sediment transport simulations on the Sendai Plain, Japan

NASA Astrophysics Data System (ADS)

Watanabe, Masashi; Goto, Kazuhisa; Bricker, Jeremy D.; Imamura, Fumihiko

2018-02-01

We examined the quantitative difference in the distribution of tsunami and storm deposits based on numerical simulations of inundation and sediment transport due to tsunami and storm events on the Sendai Plain, Japan. The calculated distance from the shoreline inundated by the 2011 Tohoku-oki tsunami was smaller than that inundated by storm surges from hypothetical typhoon events. Previous studies have assumed that deposits observed farther inland than the possible inundation limit of storm waves and storm surge were tsunami deposits. However, confirming only the extent of inundation is insufficient to distinguish tsunami and storm deposits, because the inundation limit of storm surges may be farther inland than that of tsunamis in the case of gently sloping coastal topography such as on the Sendai Plain. In other locations, where coastal topography is steep, the maximum inland inundation extent of storm surges may be only several hundred meters, so marine-sourced deposits that are distributed several km inland can be identified as tsunami deposits by default. Over both gentle and steep slopes, another difference between tsunami and storm deposits is the total volume deposited, as flow speed over land during a tsunami is faster than during a storm surge. Therefore, the total deposit volume could also be a useful proxy to differentiate tsunami and storm deposits.

Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bala, Shashi; Kumar, Ajay; Soni, Shivani

2006-04-21

Emp, originally detected in erythroblastic islands, is expressed in numerous cell types and tissues suggesting a functionality not limited to hematopoiesis. To study the function of Emp in non-hematopoietic cells, an epitope-tagged recombinant human Emp was expressed in HEK cells. Preliminary studies revealed that Emp partitioned into both the nuclear and Triton X-100-insoluble cytoskeletal fractions in approximately a 4:1 ratio. In this study, we report investigations of Emp in the nucleus. Sequential extractions of interphase nuclei showed that recombinant Emp was present predominantly in the nuclear matrix. Immunofluorescence microscopy showed that Emp was present in typical nuclear speckles enriched withmore » the spliceosome assembly factor SC35 and partially co-localized with actin staining. Coimmunoprecipitation and GST-pull-down assays confirmed the apparent close association of Emp with nuclear actin. During mitosis, Emp was detected at the mitotic spindle/spindle poles, as well as in the contractile ring during cytokinesis. These results suggest that Emp undergoes dynamic rearrangements within the nuclear architecture that are correlated with cell division.« less

Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division.

PubMed

Bala, Shashi; Kumar, Ajay; Soni, Shivani; Sinha, Sudha; Hanspal, Manjit

2006-04-21

Emp, originally detected in erythroblastic islands, is expressed in numerous cell types and tissues suggesting a functionality not limited to hematopoiesis. To study the function of Emp in non-hematopoietic cells, an epitope-tagged recombinant human Emp was expressed in HEK cells. Preliminary studies revealed that Emp partitioned into both the nuclear and Triton X-100-insoluble cytoskeletal fractions in approximately a 4:1 ratio. In this study, we report investigations of Emp in the nucleus. Sequential extractions of interphase nuclei showed that recombinant Emp was present predominantly in the nuclear matrix. Immunofluorescence microscopy showed that Emp was present in typical nuclear speckles enriched with the spliceosome assembly factor SC35 and partially co-localized with actin staining. Coimmunoprecipitation and GST-pull-down assays confirmed the apparent close association of Emp with nuclear actin. During mitosis, Emp was detected at the mitotic spindle/spindle poles, as well as in the contractile ring during cytokinesis. These results suggest that Emp undergoes dynamic rearrangements within the nuclear architecture that are correlated with cell division.

Requirement for erythroblast-macrophage protein (Emp) in definitive erythropoiesis.

PubMed

Soni, Shivani; Bala, Shashi; Hanspal, Manjit

2008-01-01

Emp, erythroblast-macrophage protein was initially identified as a mediator of erythroblast-macrophage interactions during erythroid differentiation. More recent studies have shown that targeted disruption of Emp leads to abnormal erythropoiesis in the fetal liver, and fetal demise. To further address the activity of Emp in the hematopoietic lineage in adult bone marrow, we conducted fetal liver HSC reconstitution assay. Emp null fetal liver cells were transplanted into lethally irradiated wild-type sibling mice, and assessed the erythropoietic activity. We found that Emp null cells rescued lethally irradiated mice with efficiency comparable to that of wild-type cells. However, the recipients of Emp null cells showed abnormal erythropoiesis as indicated by the presence of persistent anemia, extensive extramedullary erythropoiesis, and increased apoptosis of erythroid precursors. Extramedullary erythropoiesis suggests perturbed interactions between the Emp-deficient hematopoietic cells and the wild-type niche. Furthermore, in spleen colony-forming unit assays, proliferation rates of the Emp null cells were greater than those of the wild-type cells. Similarly, in vitro burst-forming unit-erythroid and colony-forming unit-erythroid assays showed increased erythroid colony numbers from Emp null livers. Morphologic examination showed that Emp null CFU-E-derived erythroblasts were immature compared to those derived from wild-type CFU-Es, suggesting that loss of Emp function in erythroid cells results in impaired proliferation and terminal differentiation. These results demonstrate that Emp plays a cell intrinsic role in the erythroid lineage.

Treatment effect heterogeneity for univariate subgroups in clinical trials: Shrinkage, standardization, or else

PubMed Central

Varadhan, Ravi; Wang, Sue-Jane

2016-01-01

Treatment effect heterogeneity is a well-recognized phenomenon in randomized controlled clinical trials. In this paper, we discuss subgroup analyses with prespecified subgroups of clinical or biological importance. We explore various alternatives to the naive (the traditional univariate) subgroup analyses to address the issues of multiplicity and confounding. Specifically, we consider a model-based Bayesian shrinkage (Bayes-DS) and a nonparametric, empirical Bayes shrinkage approach (Emp-Bayes) to temper the optimism of traditional univariate subgroup analyses; a standardization approach (standardization) that accounts for correlation between baseline covariates; and a model-based maximum likelihood estimation (MLE) approach. The Bayes-DS and Emp-Bayes methods model the variation in subgroup-specific treatment effect rather than testing the null hypothesis of no difference between subgroups. The standardization approach addresses the issue of confounding in subgroup analyses. The MLE approach is considered only for comparison in simulation studies as the “truth” since the data were generated from the same model. Using the characteristics of a hypothetical large outcome trial, we perform simulation studies and articulate the utilities and potential limitations of these estimators. Simulation results indicate that Bayes-DS and Emp-Bayes can protect against optimism present in the naïve approach. Due to its simplicity, the naïve approach should be the reference for reporting univariate subgroup-specific treatment effect estimates from exploratory subgroup analyses. Standardization, although it tends to have a larger variance, is suggested when it is important to address the confounding of univariate subgroup effects due to correlation between baseline covariates. The Bayes-DS approach is available as an R package (DSBayes). PMID:26485117

Sirt6 mRNA-incorporated endothelial microparticles (EMPs) attenuates DM patient-derived EMP-induced endothelial dysfunction

PubMed Central

Jing, Tong; Ya-Shu, Kuang; Xue-Jun, Wang; Han-Jing, Hou; Yan, Lai; Yi-An, Yao; Fei, Chen; Xue-Bo, Liu

2017-01-01

Background Endothelial microparticles (EMPs) are small vesicles released by endothelial cells (ECs); they are considered biomarkers for endothelial dysfunction and therapeutic targets in diabetes-related vascular disease. Sirtuins have also been shown to play important roles in diabetes by regulating endothelial dysfunction. However, the effect of sirtuin-incorporated EMPs on their parental ECs remains unknown. Aim The present study aims to investigate the diagnostic value of EMPs in diabetes and detect the protective effects of sirtuin 6 (Sirt6) mRNA -incorporated EMPs on endothelial dysfunction. Methods EMPs were prepared from cultured HUVECs and venous blood from patients with diabetes (n=10) and from healthy volunteers (n=6) after sequential centrifugation. Adv-Sirt6 or Sirt6 siRNA was used to alter Sirt6 expression. EC angiogenesis, inflammatory phenotypes, nitric oxide (NO) formation and eNOS phosphorylation were used to evaluate endothelial dysfunction. Results The levels of EMPs in diabetic patients and high glucose-cultured HUVECs are high, whereas Sirt6 expression in plasma and EMPs is low. EMPs generated from diabetic patients or high glucose-cultured HUVECs increase inflammatory chemokine release and blunt EC angiogenesis. Furthermore, EMPs enriched with Sirt6 mRNA induces EC angiogenesis, increases eNOS phosphorylation and impedes inflammatory chemokine release. Inhibition of Sirt6 mRNA expression in EMPs by siRNA hinders angiogenesis and eNOS phosphorylation but increases cellular inflammation. Conclusion The Sirt6 mRNA-carrying EMPs may ameliorate endothelial dysfunction in diabetic patients. PMID:29371988

Sirt6 mRNA-incorporated endothelial microparticles (EMPs) attenuates DM patient-derived EMP-induced endothelial dysfunction.

PubMed

Jing, Tong; Ya-Shu, Kuang; Xue-Jun, Wang; Han-Jing, Hou; Yan, Lai; Yi-An, Yao; Fei, Chen; Xue-Bo, Liu

2017-12-26

Endothelial microparticles (EMPs) are small vesicles released by endothelial cells (ECs); they are considered biomarkers for endothelial dysfunction and therapeutic targets in diabetes-related vascular disease. Sirtuins have also been shown to play important roles in diabetes by regulating endothelial dysfunction. However, the effect of sirtuin-incorporated EMPs on their parental ECs remains unknown. The present study aims to investigate the diagnostic value of EMPs in diabetes and detect the protective effects of sirtuin 6 ( Sirt6 ) mRNA -incorporated EMPs on endothelial dysfunction. EMPs were prepared from cultured HUVECs and venous blood from patients with diabetes (n=10) and from healthy volunteers (n=6) after sequential centrifugation. Adv- Sirt6 or Sirt6 siRNA was used to alter Sirt6 expression. EC angiogenesis, inflammatory phenotypes, nitric oxide (NO) formation and eNOS phosphorylation were used to evaluate endothelial dysfunction. The levels of EMPs in diabetic patients and high glucose-cultured HUVECs are high, whereas Sirt6 expression in plasma and EMPs is low. EMPs generated from diabetic patients or high glucose-cultured HUVECs increase inflammatory chemokine release and blunt EC angiogenesis. Furthermore, EMPs enriched with Sirt6 mRNA induces EC angiogenesis, increases eNOS phosphorylation and impedes inflammatory chemokine release. Inhibition of Sirt6 mRNA expression in EMPs by siRNA hinders angiogenesis and eNOS phosphorylation but increases cellular inflammation. The Sirt6 mRNA -carrying EMPs may ameliorate endothelial dysfunction in diabetic patients.

Modeling of GIC Impacts in Different Time Scales, and Validation with Measurement Data

NASA Astrophysics Data System (ADS)

Shetye, K.; Birchfield, A.; Overbye, T. J.; Gannon, J. L.

2016-12-01

Geomagnetically induced currents (GICs) have mostly been associated with geomagnetic disturbances (GMDs) originating from natural events such as solar coronal mass ejections. There is another, man-made, phenomenon that can induce GICs in the bulk power grid. Detonation of nuclear devices at high altitudes can give rise to electromagnetic pulses (EMPs) that induce electric fields at the earth's surface. EMPs cause three types of waves on different time scales, the slowest of which, E3, can induce GICs similar to the way GMDs do. The key difference between GMDs and EMPs is the rise time of the associated electric field. E3 electric fields are in the msec. to sec. range, whereas GMD electric fields are slower (sec. to min.). Similarly, the power grid and its components also operate and respond to disturbances in various time frames, right from electromagnetic transients (eg. lightning propagation) in the micro second range to steady state power flow ( hours). Hence, different power system component models need to be used to analyze the impacts of GICs caused by GMDs, and EMPs. For instance, for the slower GMD based GICs, a steady-state (static) analysis of the system is sufficient. That is, one does not need to model the dynamic components of a power system, such as the rotating machine of a generator, or generator controls such as exciters, etc. The latter become important in the case of an E3 EMP wave, which falls in the power system transient stability time frame of msec. to sec. This talk will first give an overview of the different time scales and models associated with power system operations, and where GMD and EMPs fit in. This is helpful to develop appropriate system models and test systems for analyzing impacts of GICs from various sources, and developing mitigation measures. Example test systems developed for GMD and EMP analysis, and their key modeling and analysis differences will be presented. After the modeling is discussed, results of validating simulated GICs with GIC measurements from a utility for a recent moderate GMD event will be shown, using NSF Earthscope derived electric fields. The end goal is to validate 1) power system models used for GICs, and 2) ground models to see whether 3D ground models provide better results than the hitherto-used 1D ground models.

Elves and associated electron density changes due to cloud-to-ground and in-cloud lightning discharges

NASA Astrophysics Data System (ADS)

Marshall, R. A.; Inan, U. S.; Glukhov, V. S.

2010-04-01

A 3-D finite difference time domain model is used to simulate the lightning electromagnetic pulse (EMP) and its interaction with the lower ionosphere. Results agree with the frequently observed, doughnut-shaped optical signature of elves but show that the structure exhibits asymmetry due to the presence of Earth's ambient magnetic field. Furthermore, in-cloud (horizontal) lightning channels produce observable optical emissions without the doughnut shape and, in fact, produce a much stronger optical output for the same channel current. Electron density perturbations associated with elves are also calculated, with contributions from attachment and ionization. Results presented as a function of parameters such as magnetic field direction, dipole current orientation, altitude and amplitude, and ambient ionospheric density profile demonstrate the highly nonlinear nature of the EMP-ionosphere interaction. Ionospheric effects of a sequence of in-cloud discharges are calculated, simulating a burst of in-cloud lightning activity and resulting in large density changes in the overlying ionosphere.

A probabilistic storm surge risk model for the German North Sea and Baltic Sea coast

NASA Astrophysics Data System (ADS)

Grabbert, Jan-Henrik; Reiner, Andreas; Deepen, Jan; Rodda, Harvey; Mai, Stephan; Pfeifer, Dietmar

2010-05-01

The German North Sea coast is highly exposed to storm surges. Due to its concave bay-like shape mainly orientated to the North-West, cyclones from Western, North-Western and Northern directions together with astronomical tide cause storm surges accumulating the water in the German bight. Due to the existence of widespread low-lying areas (below 5m above mean sea level) behind the defenses, large areas including large economic values are exposed to coastal flooding including cities like Hamburg or Bremen. The occurrence of extreme storm surges in the past like e.g. in 1962 taking about 300 lives and causing widespread flooding and 1976 raised the awareness and led to a redesign of the coastal defenses which provide a good level of protection for today's conditions. Never the less the risk of flooding exists. Moreover an amplification of storm surge risk can be expected under the influence of climate change. The Baltic Sea coast is also exposed to storm surges, which are caused by other meteorological patterns. The influence of the astronomical tide is quite low instead high water levels are induced by strong winds only. Since the exceptional extreme event in 1872 storm surge hazard has been more or less forgotten. Although such an event is very unlikely to happen, it is not impossible. Storm surge risk is currently (almost) non-insurable in Germany. The potential risk is difficult to quantify as there are almost no historical losses available. Also premiums are difficult to assess. Therefore a new storm surge risk model is being developed to provide a basis for a probabilistic quantification of potential losses from coastal inundation. The model is funded by the GDV (German Insurance Association) and is planned to be used within the German insurance sector. Results might be used for a discussion of insurance cover for storm surge. The model consists of a probabilistic event driven hazard and a vulnerability module, furthermore an exposure interface and a financial module to account for specific (re-) insurance conditions. This contribution will mainly concentrate on the hazard module. The hazard is covered by an event simulation engine enabling Monte Carlo simulations. The event generation is done on-the-fly. A classification of historical storm surges is used based on observed sea water levels at gauging stations and extended literature research. To characterize the origin of storm events and storm surges caused by those, also meteorological parameters like wind speed and wind direction are being used. If high water levels along the coast are mainly caused by strong wind from particular directions as observed at the North Sea, there is a clear empirical relationship between wind and surge (where surge is defined as the wind-driven component of the sea water level) which can be described by the ATWS (Average Transformed Wind speed). The parameters forming the load at the coastal defense elements are water level and wave parameters like significant wave height, wave period and wave direction. To assess the wave characteristics at the coast the numerical model SWAN (Simulating Waves Near Shore) from TU Delft has been used. To account for different probabilities of failure and inundation the coast is split into segments with similar defense characteristics like type of defense, height, width, orientation and others. The chosen approach covers the most relevant failure mechanisms for coastal dikes induced by wave overtopping and overflow. Dune failure is also considered in the model. Inundation of the hinterland after defense failure is modeled using a simple dynamical 2d-approach resulting in distributed water depths and flood outlines for each segment. Losses can be estimated depending on the input exposure data either coordinate based for single buildings or aggregated on postal code level using a set of depths-damage functions.

Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p.

PubMed

Satoh, Tadashi; Sato, Ken; Kanoh, Akira; Yamashita, Katsuko; Yamada, Yusuke; Igarashi, Noriyuki; Kato, Ryuichi; Nakano, Akihiko; Wakatsuki, Soichi

2006-04-14

Emp46p and Emp47p are type I membrane proteins, which cycle between the endoplasmic reticulum (ER) and the Golgi apparatus by vesicles coated with coat protein complexes I and II (COPI and COPII). They are considered to function as cargo receptors for exporting N-linked glycoproteins from the ER. We have determined crystal structures of the carbohydrate recognition domains (CRDs) of Emp46p and Emp47p of Saccharomyces cerevisiae, in the absence and presence of metal ions. Both proteins fold as a beta-sandwich, and resemble that of the mammalian ortholog, p58/ERGIC-53. However, the nature of metal binding is distinct from that of Ca(2+)-dependent p58/ERGIC-53. Interestingly, the CRD of Emp46p does not bind Ca(2+) ion but instead binds K(+) ion at the edge of a concave beta-sheet whose position is distinct from the corresponding site of the Ca(2+) ion in p58/ERGIC-53. Binding of K(+) ion to Emp46p appears essential for transport of a subset of glycoproteins because the Y131F mutant of Emp46p, which cannot bind K(+) ion fails to rescue the transport in disruptants of EMP46 and EMP47 genes. In contrast the CRD of Emp47p binds no metal ions at all. Furthermore, the CRD of Emp46p binds to glycoproteins carrying high mannosetype glycans and the is promoted by binding not the addition of Ca(2+) or K(+) ion in These results suggest that Emp46p can be regarded as a Ca(2+)-independent intracellular lectin at the ER exit sites.

Epithelial Membrane Protein-2 in Human Proliferative Vitreoretinopathy and Epiretinal Membranes.

PubMed

Telander, David G; Yu, Alfred K; Forward, Krisztina I; Morales, Shawn A; Morse, Lawrence S; Park, Susanna S; Gordon, Lynn K

2016-06-01

To determine the level of epithelial membrane protein-2 (EMP2) expression in preretinal membranes from surgical patients with proliferative vitreoretinopathy (PVR) or epiretinal membranes (ERMs). EMP2, an integrin regulator, is expressed in the retinal pigment epithelium and understanding EMP2 expression in human retinal disease may help determine whether EMP2 is a potential therapeutic target. Preretinal membranes were collected during surgical vitrectomies after obtaining consents. The membranes were fixed, processed, sectioned, and protein expression of EMP2 was evaluated by immunohistochemistry. The staining intensity (SI) and percentage of positive cells (PP) in membranes were compared by masked observers. Membranes were categorized by their cause and type including inflammatory and traumatic. All of the membranes stained positive for EMP2. Proliferative vitreoretinopathy-induced membranes (all causes) showed greater expression of EMP2 than ERMs with higher SI (1.81 vs. 1.38; P = 0.07) and PP (2.08 vs. 1.54; P = 0.09). However all the PVR subgroups had similar levels of EMP2 expression without statistically significant differences by Kruskal-Wallis test. Inflammatory PVR had higher expression of EMP2 than ERMs (SI of 2.58 vs. 1.38); however, this was not statistically significant. No correlation was found between duration of PVR membrane and EMP2 expression. EMP2 was detected by RT-PCR in all samples (n = 6) tested. All studied ERMs and PVR membranes express EMP2. Levels of EMP2 trended higher in all PVR subgroups than in ERMs, especially in inflammatory and traumatic PVR. Future studies are needed to determine the role of EMP2 in the pathogenesis and treatment of various retinal conditions including PVR.

A randomized phase II trial of personalized peptide vaccine plus low dose estramustine phosphate (EMP) versus standard dose EMP in patients with castration resistant prostate cancer.

PubMed

Noguchi, Masanori; Kakuma, Tatsuyuki; Uemura, Hirotsugu; Nasu, Yasutomo; Kumon, Hiromi; Hirao, Yasuhiko; Moriya, Fukuko; Suekane, Shigetaka; Matsuoka, Kei; Komatsu, Nobukazu; Shichijo, Shigeki; Yamada, Akira; Itoh, Kyogo

2010-07-01

Personalized peptide vaccination (PPV) combined with chemotherapy could be a novel approach for many cancer patients. In this randomized study, we evaluated the anti-tumor effect and safety of PPV plus low-dose estramustine phosphate (EMP) as compared to standard-dose EMP for HLA-A2- or -A24-positive patients with castration resistant prostate cancer. Patients were randomized into groups receiving either PPV plus low-dose EMP (280 mg/day) or standard-dose EMP (560 mg/day). After disease progression, patients were switched to the opposite regime. The primary end point was progression-free survival (PFS). We randomly assigned 28 patients to receive PPV plus low-dose EMP and 29 patients to receive standard-dose EMP. Nineteen events in the PPV group and 20 events in the EMP group occurred during the first treatment. Median PFS for the first treatment was 8.5 months in the PPV group and 2.8 months in the EMP group with a hazard ratio (HR) of 0.28 (95% CI, 0.14-0.61; log-rank P = 0.0012), while there was no difference for median PFS for the second treatment. The HR for overall survival was 0.3 (95% CI, 0.1-0.91) in favor of the PPV plus low-dose EMP group (log-rank, P = 0.0328). The PPV plus low-dose EMP was well tolerated without major adverse effects and with increased levels of IgG and cytotoxic-T cell responses to the vaccinated peptides. PPV plus low-dose EMP was associated with an improvement in PSA-based PFS as compared to the standard-dose EMP alone.

Extreme storms, sea level rise, and coastal change: implications for infrastructure reliability in the Gulf of Mexico

NASA Astrophysics Data System (ADS)

Anarde, K.; Kameshwar, S.; Irza, N.; Lorenzo-Trueba, J.; Nittrouer, J. A.; Padgett, J.; Bedient, P. B.

2016-12-01

Predicting coastal infrastructure reliability during hurricane events is important for risk-based design and disaster planning, such as delineating viable emergency response routes. Previous research has focused on either infrastructure vulnerability to coastal flooding or the impact of changing sea level and landforms on surge dynamics. Here we investigate the combined impact of sea level, morphology, and coastal flooding on the reliability of highway bridges - the only access points between barrier islands and mainland communities - during future extreme storms. We forward model coastal flooding for static projections of geomorphic change using ADCIRC+SWAN. First-order parameters that are adjusted include sea level and elevation. These are varied for each storm simulation to evaluate relative impact on the reliability of bridges surrounding Freeport, TX. Simulated storms include both synthetic and historical events, which are classified by intensity using the storm's integrated kinetic energy, a metric for surge generation potential. Reliability is estimated through probability of failure - given wave and surge loads - and time inundated. Findings include that: 1) bridge reliability scales inversely with surge height, and 2) sea level rise reduces bridge reliability due to a monotonic increase in surge height. The impact of a shifting landscape on bridge reliability is more complex: barrier island rollback can increase or decrease inundation times for storms of different intensity due to changes in wind-setup and back-barrier bay interactions. Initial storm surge readily inundates the coastal landscape during large intensity storms, however the draining of inland bays following storm passage is significantly impeded by the barrier. From a coastal engineering standpoint, we determine that to protect critical infrastructure, efforts now implemented that nourish low-lying barriers may be enhanced by also armoring back-bay coastlines and elevating bridge approach ramps.

The relationship between various exposure metrics for elongate mineral particles (EMP) in the taconite mining and processing industry.

PubMed

Hwang, Jooyeon; Ramachandran, Gurumurthy; Raynor, Peter C; Alexander, Bruce H; Mandel, Jeffrey H

2014-01-01

Different dimensions of elongate mineral particles (EMP) have been proposed as being relevant to respiratory health end-points such as mesothelioma and lung cancer. In this article, a methodology for converting personal EMP exposures measured using the National Institute for Occupational Safety and Health (NIOSH) 7400/7402 methods to exposures based on other size-based definitions has been proposed and illustrated. Area monitoring for EMP in the taconite mines in Minnesota's Mesabi Iron Range was conducted using a Micro Orifice Uniform Deposit Impactor (MOUDI) size-fractionating sampler. EMP on stages of the MOUDI were counted and sized according to each EMP definition using an indirect-transfer transmission electron microscopy (ISO Method 13794). EMP were identified using energy-dispersive x-ray and electron diffraction analysis. Conversion factors between the EMP counts based on different definitions were estimated using (1) a linear regression model across all locations and (2) a location-specific ratio of the count based on each EMP definition to the NIOSH 7400/7402 count. The highest fractions of EMP concentrations were found for EMP that were 1-3 μm in length and 0.2-0.5 μm in width. Therefore, the current standard NIOSH Method 7400, which only counts EMP >5 μm in length and ≥ 3 in aspect ratio, may underestimate amphibole EMP exposures. At the same time, there was a high degree of correlation between the exposures estimated according to the different size-based metrics. Therefore, the various dimensional definitions probably do not result in different dose-response relationships in epidemiological analyses. Given the high degree of correlation between the various metrics, a result consistent with prior research, a more reasonable metric might be the measurement of all EMP irrespective of size. [Supplementary materials are available for this article. Go to the publisher's online edition of Journal of Occupational and Environmental Hygiene for the following free supplemental resource: figures detailing EMP concentration.].

Release of endothelial microparticles in patients with arterial hypertension, hypertensive emergencies and catheter-related injury.

PubMed

Sansone, Roberto; Baaken, Maximilian; Horn, Patrick; Schuler, Dominik; Westenfeld, Ralf; Amabile, Nicolas; Kelm, Malte; Heiss, Christian

2018-06-01

Circulating endothelial microparticles (EMPs) are increased in arterial hypertension. The role of physicomechanical factors that may induce EMP release in vivo is still unknown. We studied the relationship of EMPs and physicomechanical factors in stable arterial hypertension and hypertensive emergencies, and investigated the pattern of EMP release after mechanical endothelial injury. In a pilot study, 41 subjects (50% hypertensives) were recruited. EMPs were discriminated by flow-cytometry (CD31 + /41 - , CD62e + , CD144 + ). Besides blood pressure measurements, pulse-wave-analysis was performed. Flow-mediated dilation (FMD), nitroglycerin-mediated dilation (NMD), and wall-shear-stress (WSS) were measured ultrasonographically in the brachial artery; microvascular perfusion by laser-Doppler (Clinicaltrials.gov: NCT02795377). We studied patients with hypertensive emergencies before and 4 h after BP lowering by urapidil (n = 12) and studied the release of EMPs due to mechanical endothelial injury after coronary angiography (n = 10). Hypertensives exhibited increased EMPs (CD31 + /41 - , CD144 + , CD62e + ) as compared to normotensives and EMPs univariately correlated with systolic BP (SBP), augmentation index, and pulse wave velocity and inversely with FMD. CD31 + /41 - -EMPs correlated with diameter and inversely with WSS and NMD. CD62e + and CD144 + -EMPs inversely correlated with microvascular function. During hypertensive emergency, only CD62e + and CD144 + -EMPs were further elevated and FMD was decreased compared to stable hypertensives. Blood pressure lowering decreased CD62e + and CD144 + -EMPs and increased FMD. CD31 + /41 - EMPs, diameter, and WSS remained unaffected. Similar to hypertensive emergency, catheter-related endothelial injury increased only CD144 + and CD62e + -EMPs. EMP release in hypertension is complex and may involve both physicomechanical endothelial injury and activation (CD144 + , CD62e + ) and decreased wall shear stress (CD31 + /41 - ). Copyright © 2018 Elsevier B.V. All rights reserved.

Dynamic Simulation of a Wave Rotor Topped Turboshaft Engine

NASA Technical Reports Server (NTRS)

Greendyke, R. B.; Paxson, D. E.; Schobeiri, M. T.

1997-01-01

The dynamic behavior of a wave rotor topped turboshaft engine is examined using a numerical simulation. The simulation utilizes an explicit, one-dimensional, multi-passage, CFD based wave rotor code in combination with an implicit, one-dimensional, component level dynamic engine simulation code. Transient responses to rapid fuel flow rate changes and compressor inlet pressure changes are simulated and compared with those of a similarly sized, untopped, turboshaft engine. Results indicate that the wave rotor topped engine responds in a stable, and rapid manner. Furthermore, during certain transient operations, the wave rotor actually tends to enhance engine stability. In particular, there is no tendency toward surge in the compressor of the wave rotor topped engine during rapid acceleration. In fact, the compressor actually moves slightly away from the surge line during this transient. This behavior is precisely the opposite to that of an untopped engine. The simulation is described. Issues associated with integrating CFD and component level codes are discussed. Results from several transient simulations are presented and discussed.

Improvements of Storm Surge Modelling in the Gulf of Venice with Satellite Data: The ESA Due Esurge-Venice Project

NASA Astrophysics Data System (ADS)

De Biasio, F.; Bajo, M.; Vignudelli, S.; Papa, A.; della Valle, A.; Umgiesser, G.; Donlon, C.; Zecchetto, S.

2016-08-01

Among the most detrimental natural phenomena, storm surges heavily endanger the environment, the economy and the everyday life of sea-side countries and coastal zones. Considering that 120.000.000 people live in the Mediterranean area, with additional 200.000.000 presences in Summer for tourism purposes, the correct prediction of storm surges is crucial to avoid fatalities and economic losses. Earth Observation (EO) can play an important role in operational storm surge forecasting, yet it is not widely diffused in the storm surge community. In 2011 the European Space Agency (ESA), through its Data User Element (DUE) programme, financed two projects aimed at encouraging the uptake of EO data in this sector: eSurge and eSurge-Venice (eSV). The former was intended to address the issues of a wider users' community, while the latter was focused on a restricted geographical area: the northern Adriatic Sea and the Gulf of Venice. Among the objectives of the two projects there were a number of storm surge hindcast experiments using satellite data, to demonstrate the improvements on the surge forecast brought by EO. We report here the results of the hindcast experiments of the eSV project. They were aimed to test the sensitivity of a storm surge model to a forcing wind field modified with scatterometer data in order to reduce the bias between simulated and observed winds. Hindcast experiments were also performed to test the response of the storm surge model to the assimilation, with a dual 4D-Var system, of satellite altimetry observations as model errors of the initial state of the sea surface level. Remarkable improvements on the storm surge forecast have been obtained for what concerns the modified model wind forcing. Encouraging results have been obtained also in the assimilation experiments.

Effects of electromagnetic pulse on serum element levels in rat.

PubMed

Li, Kangchu; Ma, Shirong; Ren, Dongqing; Li, Yurong; Ding, Guirong; Liu, Junye; Guo, Yao; Guo, Guozhen

2014-04-01

Electromagnetic pulse (EMP) was a potentially harmful factor to the human body, and a biological dosimetry to evaluate effects of EMP is necessary. Little is known about effects of EMP on concentration of macro and trace elements in serum so far. In this study, Sprague-Dawley rats were randomly divided into 50-kV/m EMP-exposed group (n = 10), 100-kV/m EMP-exposed group (n = 10), 200-kV/m EMP-exposed group (n = 40), and the sham-exposed group (n = 20). The macro and trace element concentrations in serum were examined at 6, 12, 24, and 48 h after EMP exposure at different electric field intensities. Compared with the sham-exposed groups, the concentration of sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), zinc (Zn), copper (Cu), iron (Fe), selenium (Se), and manganese (Mn) in rat serum was not changed significantly within 48 h after 200 pulses of EMP exposure at electric field intensity of 50, 100, and 200 kV/m although the K level was decreased and the Ca level was increased with the electric field intensity of EMP increasing. In addition, there was a tendency that the Zn level was decreased with the time going on within 48 h after EMP exposure. Under our experimental conditions, EMP exposure cannot affect the concentration of macro and trace elements in rat serum. There was no time-effect or dose-effect relationship between EMP exposure and serum element levels. The macro and trace elements in serum are not suitable endpoints of biological dosimetry of EMP.

EMP2 regulates angiogenesis in endometrial cancer cells through induction of VEGF

PubMed Central

Gordon, L K; Kiyohara, M; Fu, M; Braun, J; Dhawan, P; Chan, A; Goodglick, L; Wadehra, M

2013-01-01

Understanding tumor-induced angiogenesis is a challenging problem with important consequences for the diagnosis and treatment of cancer. In this study, we define a novel function for epithelial membrane protein-2 (EMP2) in the control of angiogenesis. EMP2 functions as an oncogene in endometrial cancer, and its expression has been linked to decreased survival. Using endometrial cancer xenografts, modulation of EMP2 expression resulted in profound changes to the tumor microvasculature. Under hypoxic conditions, upregulation of EMP2 promoted vascular endothelial growth factors (VEGF) expression through a HIF-1α-dependent pathway and resulted in successful capillary-like tube formation. In contrast, reduction of EMP2 correlated with reduced HIF-1α and VEGF expression with the net consequence of poorly vascularized tumors in vivo. We have previously shown that targeting of EMP2 using diabodies in endometrial cancer resulted in a reduction of tumor load, and since then we have constructed a fully human EMP2 IgG1. Treatment of endometrial cancer cells with EMP2-IgG1 reduced tumor load with a significant improvement in survival. These results support the role of EMP2 in the control of the tumor microenvironment and confirm the cytotoxic effects observed by EMP2 treatment in vivo. PMID:23334331

Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation.

PubMed

Soni, Shivani; Bala, Shashi; Kumar, Ajay; Hanspal, Manjit

2007-01-01

Erythroblast macrophage protein (Emp) mediates the attachment of erythroid cells to macrophages and is required for normal differentiation of both cell lineages. In erythroid cells, Emp is believed to be involved in nuclear extrusion, however, its role in macrophage differentiation is unknown. Information on the changes in the expression level and subcellular distribution of Emp in differentiating macrophages is essential for understanding the function of Emp. Macrophages of varying maturity were examined by immunofluorescence microscopy and biochemical methods. Our data show that Emp is expressed in all stages of maturation, but its localization pattern changes dramatically during maturation: in immature macrophages, a substantial fraction of Emp is associated with the nuclear matrix, whereas in more mature cells, Emp is expressed largely at cell surface. Pulse-chase experiments show that nascent Emp migrates intracellularly from the cytoplasm to the plasma membrane more efficiently in mature macrophages than in immature cells. Incubation of erythroid cells with macrophages in culture shows that erythroid cells attach to mature macrophages but not to immature macrophage precursors. Together, our data show that the temporal and spatial expression of Emp correlates with its role in erythroblastic island formation and suggest that Emp may be involved in multiple cellular functions.

*CHANGING PATTERN OF THE SUBCELLULAR DISTRIBUTION OF ERYTHROBLAST MACROPHAGE PROTEIN (EMP) DURING MACROPHAGE DIFFERENTIATION

PubMed Central

Soni, Shivani; Bala, Shashi; Kumar, Ajay; Hanspal, Manjit

2007-01-01

Erythroblast macrophage protein (Emp), mediates the attachment of erythroid cells to macrophages, and is required for normal differentiation of both cell lineages. In erythroid cells Emp is believed to be involved in nuclear extrusion however, its role in macrophage differentiation is unknown. Information on the changes in the expression level and subcellular distribution of Emp in differentiating macrophages is essential for understanding the function of Emp. Macrophages of varying maturity were examined by immunofluorescence microscopy and biochemical methods. Our data shows that Emp is expressed in all stages of maturation, but its localization pattern changes dramatically during maturation: in immature macrophages, a substantial fraction of Emp is associated with the nuclear matrix, whereas in more mature cells, Emp is expressed largely at cell surface. Pulse-chase experiments show that nascent Emp migrates intracellularly from the cytoplasm to the plasma membrane more efficiently in mature macrophages than in immature cells. Incubation of erythroid cells with macrophages in culture show that erythroid cells attach to mature macrophages but not to immature macrophage precursors. Together, our data shows that the temporal and spatial expression of Emp correlates with its role in erythroblastic island formation, and suggests that Emp may be involved in multiple cellular functions. PMID:17071116

Storm surges formation in the White and Barents Seas

NASA Astrophysics Data System (ADS)

Arkhipkin, Victor; Dobrolyubov, Sergey; Korablina, Anastasia; Myslenkov, Stanislav

2016-04-01

Investigation of storm surges in the Arctic seas are of high priority in Russia due to the active development of offshore oil and gas, construction of facilities in the coastal zone, as well as for the safety of navigation. It is important to study the variability of surges, to predict this phenomena and subsequent economic losses, thus including such information into the Russian Arctic Development Program 2020. Surges in the White and Barents Seas are caused mainly by deep cyclones of two types: "diving" from the north (88% of all cyclones) and western. The average height of the storm surges in the White Sea is 0.6-0.9 m. An average duration of storm surges is about 80 hours. Mathematical modeling is used to analyze the characteristics of storm surges formation in the Dvina Bay of the White Sea, and in the Varandey village on the Barents Sea coast. Calculating storm surge heights in the White and Barents seas is performed using the ADCIRC model on an unstructured grid with a step from 20 km in the Barents Sea to 100 m in the White Sea. Unstructured grids allowed keeping small features of the coastline of the White and Barents seas, small islands and shallow banks, and assessing their impact on the development and transformation of wind-generated waves. The ADCIRC model used data of wind field reanalysis CFSv2. The storm surges were simulated for the time period from 1979 to 2010 and included scenarios with / without direct atmospheric pressure forcing, waves and tides. Numerical experiments have revealed distribution of storm surges in channels of the Northern Dvina River delta. The storm surges spreads in the model from the north-north-west of the Dvina Bay. As storm surge moves from the wellhead to the seaside estuary of the Northern Dvina (district Solombala), its height increases from 0.5 to 2 m. We also found a non-linear interaction of the surge and tide during the phase of surge destruction. This phenomenon is the highest in the period of low water, and the smallest in the period full of water. Analysis of storm surges in the Varandey village (the southern part of the Barents Sea) showed that the maximum height of storm surge reached 2.9 m in this region in July, 2010. The work performed was supported by the RSCF (grant № 14-37-00038)

Monitoring and simulation of salinity changes in response to tide and storm surges in a sandy coastal aquifer system

NASA Astrophysics Data System (ADS)

Huizer, S.; Karaoulis, M. C.; Oude Essink, G. H. P.; Bierkens, M. F. P.

2017-08-01

Tidal dynamics and especially storm surges can have an extensive impact on coastal fresh groundwater resources. Combined with the prospect of sea-level rise and the reliance of many people on these resources, this demonstrates the need to assess the vulnerability of coastal areas to these threats. In this study, we investigated the impact of tides and storm surges on coastal groundwater at a pilot location on the Dutch coast (viz., the Sand Engine). To monitor changes in groundwater salinity under a variety of conditions, we performed automated measurements with electrical resistivity tomography for a period of 2 months between November 2014 and January 2015. The obtained resistivity images were converted to salinity images, and these images served effectively as observations of the impact of tidal fluctuations, saltwater overwash during storm surges, and the recovery of the freshwater lens after land-surface inundations. Most of the observed changes in groundwater head and salinity could be reproduced with a two-dimensional variable-density groundwater flow and salt transport model. This shows that groundwater models can be used to make accurate predictions of the impact of tides and storm surges on fresh groundwater resources, given a thorough understanding of the (local) system. Comparisons of measurements and model simulations also showed that morphological changes and wave run-up can have a strong impact on the extent of land-surface inundations in (low-elevation) dynamic coastal environments, and can therefore substantially affect coastal fresh groundwater resources.

Using climate response functions in analyzing electricity production variables. A case study from Norway.

NASA Astrophysics Data System (ADS)

Tøfte, Lena S.; Martino, Sara; Mo, Birger

2016-04-01

This study analyses whether and to which extent today's hydropower system and reservoirs in Mid-Norway are able to balance new intermittent energy sources in the region, in both today's and tomorrow's climate. We also investigate if the electricity marked model EMPS gives us reasonable results also when run in a multi simulation mode without recalibration. Climate related energy (CRE) is influenced by the weather, the system for energy production and transport, and by market mechanisms. In the region of Mid-Norway, nearly all power demand is generated by hydro-electric facilities. Due to energy deficiency and limitations in the power grid the region experiences a deficit of electricity. The region is likely to experience considerable investments in wind power and small-scale hydropower and the transmission grid within and out of the region will probably be extended, so this situation might change. In addition climate change scenarios for the region agree on higher temperatures, more precipitation in total and a larger portion of the precipitation coming as rain instead of snow, as well as we expect slightly higher wind speed and more storms during the winter. Changing temperatures will also change the electricity demand. EMPS is a tool for forecasting and planning in electricity markets, developed for optimization and simulation of hydrothermal power systems with a considerable share of hydro power. It takes into account transport constraints and hydrological differences between major areas or regional subsystems. During optimization the objective is to minimize the expected cost in the whole system subject to all constraints. Incremental water values (marginal costs for hydropower) are computed for each area using stochastic dynamic programming. A heuristic approach is used to treat the interaction between areas. In the simulation part of the model total system costs are minimized week by week for each climate scenario in a linear problem formulation. A detailed representation of hydropower is included and total hydro power production for each area is calculated, and the production is distributed among all available plants within each area. During simulation, the demand is affected by prices and temperatures. 6 different infrastructure scenarios of wind and power line development are analyzed. The analyses are done by running EMPS calibrated for today's situation for 11*11*8 different combinations of altered weather variables (temperature, precipitation and wind) describing different climate change scenarios, finding the climate response function for every EMPS-variable according the electricity production, such as prices and income, energy balances (supply, consumption and trade), overflow losses, probability of curtailment etc .

Analysis and simulation of propagule dispersal and salinity intrusion from storm surge on the movement of a marsh–mangrove ecotone in South Florida

USGS Publications Warehouse

Jiang, Jiang; DeAngelis, Donald L.; Anderson, Gordon H.; Smith, Thomas J.

2014-01-01

Coastal mangrove–freshwater marsh ecotones of the Everglades represent transitions between marine salt-tolerant halophytic and freshwater salt-intolerant glycophytic communities. It is hypothesized here that a self-reinforcing feedback, termed a “vegetation switch,” between vegetation and soil salinity, helps maintain the sharp mangrove–marsh ecotone. A general theoretical implication of the switch mechanism is that the ecotone will be stable to small disturbances but vulnerable to rapid regime shifts from large disturbances, such as storm surges, which could cause large spatial displacements of the ecotone. We develop a simulation model to describe the vegetation switch mechanism. The model couples vegetation dynamics and hydrologic processes. The key factors in the model are the amount of salt-water intrusion into the freshwater wetland and the passive transport of mangrove (e.g., Rhizophora mangle) viviparous seeds or propagules. Results from the model simulations indicate that a regime shift from freshwater marsh to mangroves is sensitive to the duration of soil salinization through storm surge overwash and to the density of mangrove propagules or seedlings transported into the marsh. We parameterized our model with empirical hydrologic data collected from the period 2000–2010 at one mangrove–marsh ecotone location in southwestern Florida to forecast possible long-term effects of Hurricane Wilma (24 October 2005). The model indicated that the effects of that storm surge were too weak to trigger a regime shift at the sites we studied, 50 km south of the Hurricane Wilma eyewall, but simulations with more severe artificial disturbances were capable of causing substantial regime shifts.

Epithelial Membrane Protein-2 is a Novel Therapeutic Target in Ovarian Cancer

PubMed Central

Fu, Maoyong; Maresh, Erin L.; Soslow, Robert A.; Alavi, Mohammad; Mah, Vei; Zhou, Qin; Iasonos, Alexia; Goodglick, Lee; Gordon, Lynn K.; Braun, Jonathan; Wadehra, Madhuri

2010-01-01

Purpose The tetraspan protein epithelial membrane protein-2 (EMP2) has been shown to regulate the surface display and signaling from select integrin pairs, and it was recently identified as a prognostic biomarker in human endometrial cancer. In this study, we assessed the role of EMP2 in human ovarian cancer. Experimental Design We examined the expression of EMP2 within a population of women with ovarian cancer using tissue microarray assay technology. We evaluated the efficacy of EMP2-directed antibody therapy using a fully human recombinant bivalent antibody fragment (diabody) in vitro and ovarian cancer xenograft models in vivo. Results EMP2 was found to be highly expressed in over 70% of serous and endometrioid ovarian tumors compared to non-malignant ovarian epithelium using a human ovarian cancer tissue microarray. Using anti-EMP2 diabody, we evaluated the in vitro response of 9 human ovarian cancer cell lines with detectable EMP2 expression. Treatment of human ovarian cancer cell lines with anti-EMP2 diabodies induced cell death and retarded cell growth, and these response rates correlated with cellular EMP2 expression. We next assessed the effects of anti-EMP2 diabodies in mice bearing xenografts from the ovarian endometrioid carcinoma cell line OVCAR5. Anti-EMP2 diabodies significantly suppressed tumor growth and induced cell death in OVCAR5 xenografts. Conclusions These findings indicate that EMP2 is expressed in the majority of ovarian tumors and it may be a feasible target in vivo. PMID:20670949

Numerical simulation of electromagnetic surface treatment

NASA Astrophysics Data System (ADS)

Sonde, Emmanuel; Chaise, Thibaut; Nelias, Daniel; Robin, Vincent

2018-01-01

Surface treatment methods, such as shot peening or laser shock peening, are generally used to introduce superficial compressive residual stresses in mechanical parts. These processes are carried out during the manufacturing steps or for the purpose of repairing. The compressive residual stresses prevent the initiation and growth of cracks and thus improve the fatigue life of mechanical components. Electromagnetic pulse peening (EMP) is an innovative process that could be used to introduce compressive residual stresses in conductive materials. It acts by generating a high transient electromagnetic field near the working surface. In this paper, the EMP process is presented and a sequentially coupled electromagnetic and mechanical model is developed for its simulation. This 2D axisymmetric model is set up with the commercial finite element software SYSWELD. After description and validation, the numerical model is used to simulate a case of introducing residual stresses of compression in a nickel-based alloy 690 thick sample, by the means of electromagnetic pulses. The results are presented in terms of effective plastic strain and residual mean stress. The influence of the process parameters, such as current intensity and frequency, on the results is analyzed. Finally, the predictability of the process is shown by several correlation studies.

Role of tumour necrosis factor receptor-1 and nuclear factor-κB in production of TNF-α-induced pro-inflammatory microparticles in endothelial cells.

PubMed

Lee, S K; Yang, S-H; Kwon, I; Lee, O-H; Heo, J H

2014-09-02

Tumour necrosis factor-α (TNF-α) is upregulated in many inflammatory diseases and is also a potent agent for microparticle (MP) generation. Here, we describe an essential role of TNF-α in the production of endothelial cell-derived microparticles (EMPs) in vivo and the function of TNF-α-induced EMPs in endothelial cells. We found that TNF-α rapidly increased blood levels of EMPs in mice. Treatment of human umbilical vein endothelial cells (HUVECs) with TNF-α also induced EMP formation in a time-dependent manner. Silencing of TNF receptor (TNFR)-1 or inhibition of the nuclear factor-κB (NF-κB) in HUVECs impaired the production of TNF-α-induced EMP. Incubation of HUVECs with PKH-67-stained EMPs showed that endothelial cells readily engulfed EMPs, and the engulfed TNF-α-induced EMPs promoted the expression of pro-apoptotic molecules and upregulated intercellular adhesion molecule-1 level on the cell surface, which led to monocyte adhesion. Collectively, our findings indicate that the generation of TNF-α-induced EMPs was mediated by TNFR1 or NF-κB and that EMPs can contribute to apoptosis and inflammation of endothelial cells.

Effect of a brief emergency medicine education course on emergency department work intensity of family physicians.

PubMed

Vaillancourt, Samuel; Schultz, Susan E; Leaver, Chad; Stukel, Thérèse A; Schull, Michael J

2013-01-01

Recently, many Canadian emergency departments (EDs) have struggled with physician staffing shortages. In 2006, the Ontario Ministry of Health and Long-Term Care funded a brief "emergency medicine primer" (EMP) course for family physicians to upgrade or refresh skills, with the goal of increasing their ED work intensity. We sought to determine the effect of the EMP on the ED work intensity of family physicians. A retrospective longitudinal study was conducted of the ED work of 239 family physicians in the 2 years before and after a minimum of 6 months and up to 2 years from completing an EMP course in 2006 to 2008 compared to non-EMP physicians. ED work intensity was defined as the number of ED shifts per month and the number of ED patients seen per month. We conducted two analyses: a before and after comparison of all EMP physicians and a matched cohort analysis matching each EMP physician to four non-EMP physicians on sex, year of medical school graduation, rurality, and pre-EMP ED work intensity. Postcourse, EMP physicians worked 0.5 more ED shifts per month (13% increase, p  =  0.027). Compared to their matched controls, EMP physicians worked 0.7 more shifts per month (13% increase, p  =  0.0032) and saw 15 more patients per month (17% increase, p  =  0.0008) compared to matched non-EMP physicians. The greatest increases were among EMP physicians who were younger, were urban, had previous ED experience, or worked in a high-volume ED. The effect of the EMP course was negligible for physicians with no previous ED experience or working in rural areas. The EMP course is associated with modest increases in ED work intensity among some family physicians, in particular younger physicians in urban areas. No increase was seen among physicians without previous ED experience or working in rural areas.

The effects of electromagnetic pulses (EMP) on the bioactivity of insulin and a preliminary study of mechanism.

PubMed

Chen, Yong Bin; Li, Jing; Qi, Yuhong; Miao, Xia; Zhou, Yongchun; Ren, Dongqing; Guo, G Z

2010-01-01

To investigate the effects of electromagnetic pulse (EMP) exposure on the bioactivity of insulin and a preliminary mechanism for these effects. A tapered parallel plate Gigahertz Transverse Electromagnetic (GTEM) cell with a flared rectangular coaxial transmission line was used to expose the insulin solution to EMP. Concurrent sham-exposed insulin solutions were used as a control. The effect of EMP-exposed insulin on fasting blood glucose levels of type I diabetes model mice, the effect of EMP on binding affinity between insulin and its receptor and the effect of EMP on insulin's fluorescence intensity were detected, respectively. (i) After EMP exposure, compared with sham-exposed insulin, the bioactivity of insulin in decreasing fasting blood glucose levels in type I diabetes model mice was reduced significantly (p = 0.023). (ii) Compared with sham-exposed insulin group, the percentage fluorescein isothiocyannate (FITC) labelling of HL-7702 cells was significantly reduced in the EMP-exposed insulin group (22.7-13.8%, respectively). (iii) Compared with sham-exposed insulin, the fluorescence intensity was significantly reduced in EMP-exposed insulin (p < 0.001). EMP exposure significantly decreased the bioactivity of insulin to reduce the blood glucose levels in type I diabetic mice. This could be due to a decreased binding affinity between insulin and its receptor. This mechanism could involve an alteration of insulin's' conformation caused by EMP exposure.

Development of High Level Electrical Stress Failure Threshold and Prediction Model for Small Scale Junction Integrated Circuits

DTIC Science & Technology

1978-09-01

AWACS EMP Guidelines presents two different models to predict the damage pcwer of the dev-ce and the circuit damage EMP voltage ( VEMP ). Neither of...calculated as K P~ I V BD 6. The damage EMP voltage ( VEMP ) is calculated KZ EMP +IZ =D +BD VBD1F 7. The damage EMP voltage is calculated for collector

Effect of Electromagnetic Pulses (EMP) on associative learning in mice and a preliminary study of mechanism.

PubMed

Chen, Yong Bin; Li, Jing; Liu, Jun Ye; Zeng, Li Hua; Wan, Yi; Li, Yu Rong; Ren, Dongqing; Guo, G Z

2011-12-01

To investigate the effects of electromagnetic pulses (EMP) on associative learning in mice and test a preliminary mechanism for these effects. A tapered parallel plate gigahertz transverse electromagnetic (GTEM) cell with a flared rectangular coaxial transmission line was used to expose male BALB/c mice to EMP (peak-intensity 400 kV/m, rise-time 10 ns, pulse-width 350 ns, 0.5 Hz and total 200 pulses). Concurrent sham-exposed mice were used as a control. Associative learning, oxidative stress in the brain, serum chemistry and the protective action of tocopherol monoglucoside (TMG) in mice were measured, respectively. (1) Twelve hour and 1 day post EMP exposure associative learning was reduced significantly compared with sham control (p<0.05) but recovered at 2 d post EMP exposure. (2) Compared with the sham control, lipid peroxidation of brain tissue and chemiluminescence (CL) intensity increased significantly (p<0.05), while the activity of the antioxidant enzymes Superoxide Dismutase [SOD], Glutathione [GSH], Glutathione Peroxidase [GSH-Px], Catalase [CAT]) decreased significantly (p<0.05) at 3 h, 6 h, 12 h and 1 d post EMP exposure. All these parameters recovered at 2 d post EMP exposure. (3) No significant differences between the sham control group and EMP exposed group were observed in serum cholesterol and triglycerides. (4) Pretreatment of mice with TMG showed protective effects to EMP exposure. EMP exposure significantly decreased associative learning in mice and TMG acted as an effective protective agent from EMP exposure. This mechanism could involve an increase of oxidative stress in brain by EMP exposure.

EMP2 is a novel therapeutic target for endometrial cancer stem cells

PubMed Central

Kiyohara, Meagan H.; Dillard, Christen; Tsui, Jessica; Kim, Sara Ruth; Lu, Jianyi; Sachdev, Divya; Goodglick, Lee; Tong, Maomeng; Torous, Vanda Farahmand; Aryasomayajula, Chinmayi; Wang, Wei; Najafzadeh, Parisa; Gordon, Lynn K.; Braun, Jonathan; McDermott, Sean; Wicha, Max S.; Wadehra, Madhuri

2017-01-01

Previous studies have suggested that overexpression of the oncogenic protein epithelial membrane protein-2 (EMP2) correlates with endometrial carcinoma progression and ultimately poor survival from disease. To understand the role of EMP2 in the etiology of disease, gene analysis was performed to show transcripts that are reciprocally regulated by EMP2 levels. In particular, EMP2 expression correlates with and helps regulate the expression of several cancer stem cell associated markers including aldehyde dehydrogenase 1 (ALDH1). ALDH expression significantly promotes tumor initiation and correlates with the levels of EMP2 expression in both patient samples and tumor cell lines. As therapy against CSCs in endometrial cancer is lacking, the ability of anti-EMP2 IgG1 therapy to reduce primary and secondary tumor formation using xenograft HEC1A models was determined. Anti-EMP2 IgG1 reduced the expression and activity of ALDH and correspondingly reduced both primary and secondary tumor load. Our results collectively suggest that anti-EMP2 therapy may be a novel method of reducing endometrial cancer stem cells. PMID:28604744

Climate projections of spatial variations in coastal storm surges along the Gulf of Mexico and U.S. east coast

NASA Astrophysics Data System (ADS)

Yao, Zhigang; Xue, Zuo; He, Ruoying; Bao, Xianwen; Xie, Jun; Ge, Qian

2017-02-01

Using statistically downscaled atmospheric forcing, we performed a numerical investigation to evaluate future climate's impact on storm surges along the Gulf of Mexico and U.S. east coast. The focus is on the impact of climatic changes in wind pattern and surface pressure while neglecting sea level rise and other factors. We adapted the regional ocean model system (ROMS) to the study region with a mesh grid size of 7-10 km in horizontal and 18 vertical layers. The model was validated by a hindcast of the coastal sea levels in the winter of 2008. Model's robustness was confirmed by the good agreement between model-simulated and observed sea levels at 37 tidal gages. Two 10-year forecasts, one for the IPCC Pre-Industry (PI) and the other for the A1FI scenario, were conducted. The differences in model-simulated surge heights under the two climate scenarios were analyzed. We identified three types of responses in extreme surge heights to future climate: a clear decrease in Middle Atlantic Bight, an increase in the western Gulf of Mexico, and non-significant response for the remaining area. Such spatial pattern is also consistent with previous projections of sea surface winds and ocean wave heights.

Hot gas ingestion effects on fuel control surge recovery and AH-1 rotor drive train torque spikes

NASA Technical Reports Server (NTRS)

Tokarski, Frank; Desai, Mihir; Books, Martin; Zagranski, Raymond

1994-01-01

This report summarizes the work accomplished through computer simulation to understand the impact of the hydromechanical turbine assembly (TA) fuel control on rocket gas ingestion induced engine surges on the AH-1 (Cobra) helicopter. These surges excite the lightly damped torsional modes of the Cobra rotor drive train and can cause overtorqueing of the tail rotor shaft. The simulation studies show that the hydromechanical TA control has a negligible effect on drive train resonances because its response is sufficiently attenuated at the resonant frequencies. However, a digital electronic control working through the TA control's separate, emergency fuel metering system has been identified as a solution to the overtorqueing problem. State-of-the-art software within the electronic control can provide active damping of the rotor drive train to eliminate excessive torque spikes due to any disturbances including engine surges and aggressive helicopter maneuvers. Modifications to the existing TA hydromechanical control are relatively minor, and existing engine sensors can be utilized by the electronic control. Therefore, it is concluded that the combination of full authority digital electronic control (FADEC) with hydromechanical backup using the existing TA control enhances flight safety, improves helicopter performance, reduces pilot workload, and provides a substantial payback for very little investment.

Typhoon Haiyan-Induced Storm Surge Simulation in Metro Manila Using High-Resolution LiDAR Topographic Data

NASA Astrophysics Data System (ADS)

Santiago, J. T.

2015-12-01

Storm surge is the abnormal rise in sea water over and above astronomical tides due to a forthcoming storm. Developing an early warning system for storm surges is vital due to the high level of hazard they might cause. On 08 November 2013, Typhoon Haiyan generated storm surges that killed over 6,000 people in the central part of the Philippines. The Nationwide Operational Assessment of Hazards under the Department of Science and Technology was tasked to create storm surge hazard maps for the country's coastal areas. The research project aims to generate storm surge hazard maps that can be used for disaster mitigation and planning. As part of the research, the team explored a scenario wherein a tropical cyclone hits the Metro Manila with strength as strong as Typhoon Haiyan. The area was chosen primarily for its political, economic and cultural significance as the country's capital. Using Japan Meteorological Agency Storm Surge model, FLO2D flooding software, LiDAR topographic data, and GIS technology, the effects of a Haiyan-induced tropical cyclone passing through Metro Manila was examined. The population affected, number of affected critical facilities, and potential evacuation sites were identified. The outputs of this study can be used by the authorities as basis for policies that involve disaster risk reduction and management.

Influence of irradiation on release of endothelial microparticles (EMP) in vitro.

PubMed

Neuber, Christin; Pufe, Johanna; Pietzsch, Jens

2015-01-01

Survivors of Hodgkin's disease as well as of breast and lung cancer are at risk of radiation-associated cardiovascular disease. Recent studies demonstrated a correlation between cardiovascular risk factors and circulating endothelial microparticles (EMP) and thereby suggest increased EMP levels in circulation to be an early biomarker of endothelial dysfunction and cardiovascular risk. This prompted us to analyze the amount of EMP released by human aortic endothelial cells (HAEC) after exposure to different doses of X-ray (0.4, 2, 4, 6, and 20 Gy) using antibodies against the endothelial cell markers CD31, CD144, and CD146 by flow cytometry. In this pilot experiment only CD146 proved appropriate for quantification of HAEC-derived EMP. Exposure of HAEC to different doses of X-ray did not significantly influence formation of CD146-positive EMP. However, low doses (0.4 Gy) tended to decrease EMP formation, whereas higher doses (2 or 4 Gy) slightly increased release of CD146-positive EMP. By contrast, inflammatory activation of HAEC by TPA significantly increased EMP release about 15-fold (P <  0.01). In conclusion, under the present experimental conditions EMP did not prove a suitable biomarker for radiation-induced endothelial dysfunction in vitro.

Dynamics of sea level rise and coastal flooding on a changing landscape

NASA Astrophysics Data System (ADS)

Bilskie, M. V.; Hagen, S. C.; Medeiros, S. C.; Passeri, D. L.

2014-02-01

Standard approaches to determining the impacts of sea level rise (SLR) on storm surge flooding employ numerical models reflecting present conditions with modified sea states for a given SLR scenario. In this study, we advance this paradigm by adjusting the model framework so that it reflects not only a change in sea state but also variations to the landscape (morphologic changes and urbanization of coastal cities). We utilize a numerical model of the Mississippi and Alabama coast to simulate the response of hurricane storm surge to changes in sea level, land use/land cover, and land surface elevation for past (1960), present (2005), and future (2050) conditions. The results show that the storm surge response to SLR is dynamic and sensitive to changes in the landscape. We introduce a new modeling framework that includes modification of the landscape when producing storm surge models for future conditions.

Clinical CVVH model removes endothelium-derived microparticles from circulation

PubMed Central

Abdelhafeez, Abdelhafeez H.; Jeziorczak, Paul M.; Schaid, Terry R.; Hoefs, Susan L.; Kaul, Sushma; Nanchal, Rahul; Jacobs, Elizabeth R.; Densmore, John C.

2014-01-01

Background Endothelium-derived microparticles (EMPs) are submicron vesicles released from the plasma membrane of endothelial cells in response to injury, apoptosis or activation. We have previously demonstrated EMP-induced acute lung injury (ALI) in animal models and endothelial barrier dysfunction in vitro. Current treatment options for ALI are limited and consist of supportive therapies. We hypothesize that standard clinical continuous venovenous hemofiltration (CVVH) reduces serum EMP levels and may be adapted as a potential therapeutic intervention. Materials and methods EMPs were generated from plasminogen activation inhibitor-1 (PAI-1)-stimulated human umbilical vein endothelial cells (HUVECs). Flow cytometric analysis was used to characterize EMPs as CD31- and annexin V-positive events in a submicron size gate. Enumeration was completed against a known concentration of latex beads. Ultimately, a concentration of ~650,000 EMP/mL perfusate fluid (total 470 mL) was circulated through a standard CVVH filter (pore size 200 μm, flow rate 250 mL/hr) for a period of 70 minutes. 0.5 mL aliquots were removed at 5- to 10-minute intervals for flow cytometric analysis. EMP concentration in the dialysate was measured at the end of 4 hours to better understand the fate of EMPs. Results A progressive decrease in circulating EMP concentration was noted using standard CVVH at 250 mL/hr (a clinical standard rate) from a 470 mL volume modelling a patient's circulation. A 50% reduction was noted within the first 30 minutes. EMPs entering the dialysate after 4 hours were 5.7% of the EMP original concentration. Conclusion These data demonstrate that standard CVVH can remove EMPs from circulation in a circuit modelling a patient. An animal model of hemofiltration with induction of EMP release is required to test the therapeutic potential of this finding and potential of application in early treatment of ALI. PMID:24596654

[Effect of EMP-1 gene on human esophageal cancer cell line].

PubMed

Wang, Hai-tao; Liu, Zhi-hua; Wang, Xiu-qin; Wu, Min

2002-03-01

EMP-1 was selected from a series of differential expressed genes obtained from cDNA microarray in the authors' lab. Epithelial membrane pnteiu-1 gene (EMP-1) was expressed 6 fold lower in esophageal cancer than in normal tissue. The authors further designed the experiment to study the effect of human EMP-1 gene on human esophageal cancer cell line in order to explain the function of this gene on the carcinogensis and progression esophageal cancer. EMP-1 gene was cloned into eukaryotic vector and transfected into the human esophageal cancer cell line. The transfection effect was qualified by Western blot and RT-PCR method. The cell growth curve was observed and the cell cycle was checked by FACS method. EMP-1 was transfected into EC9706 cell line and its expression was up-regulated. The cell growth is accelerated and expression of EMP-1 is linked to induction of S phase arrest. EMP-1 gene has some relationship with carcinogenesis of esophagus.

Effects of electromagnetic pulse (EMP) on cardiac pacemakers. Final report, Nov 88-Oct 89

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ellis, V.J.

1991-11-01

The U.S. Army Harry Diamond Laboratories' (HDL's) Woodbridge Research Facility (WRF) has conducted an investigation into the effects of electromagnetic pulse (EMP) on medical electronics. This report specifically documents the findings on the effects of WRF's Army EMP Simulator Operations (AESOP) on cardiac pacemakers (CPMs). Empirical data are furnished and compared to the results of two independent analytical studies. The studies support the conclusion that damage to CPMs that might be located near the WRF boundaries is not likely. Furthermore, any upset in a CPM's operation is considered unlikely and inconsequential to the health of the CPM wearer. Cardiac pacemakersmore » (CPMs) have experienced significant technological advancements over the last decade, evolving from simple and bulky pulse generators to the small and sophisticated computerized units implanted today. With the implementation of sensitive digital electronics in modern pacemaker designs, concerns have been expressed for the possibility of an increased sensitivity of CPMs to electromagnetic interference (EMI). To some extent these concerns have abated to the increased awareness of the EMI problem by the manufacturers, as evident in better peacemaker designs and the decline in reported malfunctions due to EMI.« less

Accelerated Time-Domain Modeling of Electromagnetic Pulse Excitation of Finite-Length Dissipative Conductors over a Ground Plane via Function Fitting and Recursive Convolution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Campione, Salvatore; Warne, Larry K.; Sainath, Kamalesh

In this report we overview the fundamental concepts for a pair of techniques which together greatly hasten computational predictions of electromagnetic pulse (EMP) excitation of finite-length dissipative conductors over a ground plane. In a time- domain, transmission line (TL) model implementation, predictions are computationally bottlenecked time-wise, either for late-time predictions (about 100ns-10000ns range) or predictions concerning EMP excitation of long TLs (order of kilometers or more ). This is because the method requires a temporal convolution to account for the losses in the ground. Addressing this to facilitate practical simulation of EMP excitation of TLs, we first apply a techniquemore » to extract an (approximate) complex exponential function basis-fit to the ground/Earth's impedance function, followed by incorporating this into a recursion-based convolution acceleration technique. Because the recursion-based method only requires the evaluation of the most recent voltage history data (versus the entire history in a "brute-force" convolution evaluation), we achieve necessary time speed- ups across a variety of TL/Earth geometry/material scenarios. Intentionally Left Blank« less

Data and numerical analysis of astronomic tides, wind-waves, and hurricane storm surge along the northern Gulf of Mexico

NASA Astrophysics Data System (ADS)

Bilskie, M. V.; Hagen, S. C.; Medeiros, S. C.; Cox, A. T.; Salisbury, M.; Coggin, D.

2016-05-01

The northern Gulf of Mexico (NGOM) is a unique geophysical setting for complex tropical storm-induced hydrodynamic processes that occur across a variety of spatial and temporal scales. Each hurricane includes its own distinctive characteristics and can cause unique and devastating storm surge when it strikes within the intricate geometric setting of the NGOM. While a number of studies have explored hurricane storm surge in the NGOM, few have attempted to describe storm surge and coastal inundation using observed data in conjunction with a single large-domain high-resolution numerical model. To better understand the oceanic and nearshore response to these tropical cyclones, we provide a detailed assessment, based on field measurements and numerical simulation, of the evolution of wind waves, water levels, and currents for Hurricanes Ivan (2004), Dennis (2005), Katrina (2005), and Isaac (2012), with focus on Mississippi, Alabama, and the Florida Panhandle coasts. The developed NGOM3 computational model describes the hydraulic connectivity among the various inlet and bay systems, Gulf Intracoastal Waterway, coastal rivers and adjacent marsh, and built infrastructure along the coastal floodplain. The outcome is a better understanding of the storm surge generating mechanisms and interactions among hurricane characteristics and the NGOM's geophysical configuration. The numerical analysis and observed data explain the ˜2 m/s hurricane-induced geostrophic currents across the continental shelf, a 6 m/s outflow current during Ivan, the hurricane-induced coastal Kelvin wave along the shelf, and for the first time a wealth of measured data and a detailed numerical simulation was performed and was presented for Isaac.

Numerical Analysis of Storm Surge and Seiche at Tokyo Bay caused by the 2 Similar Typhoons, Typhoon Phanphon and Vongfong in 2014

NASA Astrophysics Data System (ADS)

Iwamoto, T.; Takagawa, T.

2017-12-01

A long period damped oscillation, or seiche, sometimes happens inside a harbor after passing typhoon. For some cases, a maximum sea level is observed due to the superposition of astronomical tide and seiche rather than a peak of storm surge. Hence to clarify seiche factors for reducing disaster potential is important, a long-period seiche with a fundamental period of 5.46 hours in Tokyo Bay (Konishi, 2008) was investigated through numerical simulations and analyses. We examined the case of Typhoon Phanphon and Vongfong in 2014 (Hereafter Case P and V). The intensity and moving velocity were similar and the best-tracks were an arc-shaped, typical one approaching to Tokyo Bay. The track of Case V was about 1.5 degree higher latitude than that of Case P, only Typhoon Phanphon caused significant seiche.Firstly, numerical simulations for the 2 storm surges at Tokyo Bay were conducted by Regional Ocean Modeling System (ROMS) and Meso-Scale Model Grid Point Values (MSM-GPV). MSM-GPV gave the 10m wind speed and Sea Level Pressure (SLP), especially the Mean Error (ME) and Root Mean Squire Error (RMSE) of SLP were low compared to the 12 JMA observation points data (Case P: ME -0.303hPa, RMSE 1.87hPa, Case V: ME -0.285hPa, RMSE 0.74hPa). The computational results showed that the maximum of storm surge was underestimated but the difference was less than 20cm at 5 observation points in Tokyo Bay(Fig.1, 2).Then, power spectrals, coherences and phase differences of storm surges at the 5 observation points were obtained by spectral analysis of observed and simulated waveforms. For Case P, the phase-difference between the bay mouth and innermost part of Tokyo Bay was little, and coherence was almost 1(Fig.3, 4). However, for Case V, coherence was small around the fundamental period of 5.46 hours. Furthermore, Empirical Orthogonal Function (EOF) analysis of storm surge, SLP and sea surface stress were conducted. The contributions of EOF1 were above 90% for the all variables, the gradient of storm surge EOF1 was parallel to the bay axis for Case P, but about 50-degree oblique from the axis for Case V(Fig.5, 6). In addition, the EOF1 of SLP for Case P showed a concentric circle structure above Tokyo Bay, besides the structure was not appeared for Case V.

Use of Lean response to improve pandemic influenza surge in public health laboratories.

PubMed

Isaac-Renton, Judith L; Chang, Yin; Prystajecky, Natalie; Petric, Martin; Mak, Annie; Abbott, Brendan; Paris, Benjamin; Decker, K C; Pittenger, Lauren; Guercio, Steven; Stott, Jeff; Miller, Joseph D

2012-01-01

A novel influenza A (H1N1) virus detected in April 2009 rapidly spread around the world. North American provincial and state laboratories have well-defined roles and responsibilities, including providing accurate, timely test results for patients and information for regional public health and other decision makers. We used the multidisciplinary response and rapid implementation of process changes based on Lean methods at the provincial public health laboratory in British Columbia, Canada, to improve laboratory surge capacity in the 2009 influenza pandemic. Observed and computer simulating evaluation results from rapid processes changes showed that use of Lean tools successfully expanded surge capacity, which enabled response to the 10-fold increase in testing demands.

Epithelial Membrane Protein-2 Expression is an Early Predictor of Endometrial Cancer Development

PubMed Central

Habeeb, Omar; Goodglick, Lee; Soslow, Robert A.; Rao, Rajiv; Gordon, Lynn K.; Schirripa, Osvaldo; Horvath, Steve; Braun, Jonathan; Seligson, David B.; Wadehra, Madhuri

2010-01-01

BACKGROUND Endometrial cancer (EC) is a common malignancy worldwide. It is often preceded by endometrial hyperplasia, whose management and risk of neoplastic progression vary. Previously, we have shown that the tetraspan protein Epithelial Membrane Protein-2 (EMP2) is a prognostic indicator for EC aggressiveness and survival. Here we validate the expression of EMP2 in EC, and further examine whether EMP2 expression within preneoplastic lesions is an early prognostic biomarker for EC development. METHODS A tissue microarray (TMA) was constructed with a wide representation of benign and malignant endometrial samples. The TMA contains a metachronous cohort of cases from individuals who either developed or did not develop EC. Intensity and frequency of EMP2 expression were assessed using immunohistochemistry. RESULTS There was a stepwise, statistically-significant increase in the average EMP2 expression from benign to hyperplasia to atypia to EC. Furthermore, detailed analysis of EMP2 expression in potentially premalignant cases demonstrated that EMP2 positivity was a strong predictor for EC development. CONCLUSION EMP2 is an early predictor of EC development in preneoplastic lesions. In addition, combined with our previous findings, these results validate that EMP2 as a novel biomarker for EC development. PMID:20578181

Electromagnetic pulse (EMP) coupling codes for use with the vulnerability/lethality (VIL) taxonomy. Final report, June-October 1984

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mar, M.H.

1995-07-01

Based on the vulnerability Lethality (V/L) taxonomy developed by the Ballistic Vulnerability Lethality Division (BVLD) of the Survivability Lethality Analysis Directorate (SLAD), a nuclear electromagnetic pulse (EMP) coupling V/L analysis taxonomy has been developed. A nuclear EMP threat to a military system can be divided into two levels: (1) coupling to a system level through a cable, antenna, or aperture; and (2) the component level. This report will focus on the initial condition, which includes threat definition and target description, as well as the mapping process from the initial condition to damaged components state. EMP coupling analysis at a systemmore » level is used to accomplish this. This report introduces the nature of EMP threat, interaction between the threat and target, and how the output of EMP coupling analysis at a system level becomes the input to the component level analysis. Many different tools (EMP coupling codes) will be discussed for the mapping process, which correponds to the physics of phenomenology. This EMP coupling V/L taxonomy and the models identified in this report will provide the tools necessary to conduct basic V/L analysis of EMP coupling.« less

Necessity of re-evaluation of estramustine phosphate sodium (EMP) as a treatment option for first-line monotherapy in advanced prostate cancer.

PubMed

Kitamura, T

2001-02-01

Estramustine phosphate sodium (EMP) was first introduced in the early 1970s for the treatment of prostate cancer, when EMP was supposed to have the dual effect of estrogenic activity and cytotoxicity. For the following decades, it was used mainly in hormone-refractory cases, with a conventional dosage of 4-9 capsules/day, which showed a 30-35% objective response rate. However, a very limited number of cases have been reported that used EMP as a first-line monotherapy in the conventional dosage. One study showed a response rate of 82%, which is at least as effective as conventional estrogen (diethylstilbestrol; DES) monotherapy. Nevertheless, EMP was almost abandoned for the treatment of prostate cancer because of severe adverse side-effects, especially in the cardiovascular system and gastrointestinal tract. Recently, two facts have become evident. First, EMP interferes with cellular microtubule dynamics but does not show alkylating effects. Second, EMP is able to produce a complex with calcium when dairy products are taken concomitantly with EMP, resulting in a decrease in the absorption rate of EMP from the gut. Many clinical trials have been undertaken without warning against concomitant dairy product intake since the introduction of EMP. This fact will jeopardize almost all the clinical trials performed before 1990. It is considered that response rates have been underestimated and better results could have been obtained because side-effects decrease dose-dependently. Low-dose EMP monotherapy (2 capsules/day) has been performed infrequently in previously untreated advanced prostate cancer. The only large trial by the European Organization for Research and Treatment of Cancer in 1984 was biased in selecting patients. Nevertheless, the response rate of EMP is comparable to that of DES. In this study, the adverse side-effects of EMP were less than that of DES. Recently, a study was conducted at the University of Tokyo of 11 patients with advanced prostate cancer on low-dose EMP as first-line monotherapy. The study found that the mean serum prostate-specific antigen level decreased to within the normal range by day 50; mean serum testosterone, leutinizing hormone and follicle-stimulating hormone reduced to undetectable levels by day 20; and mean serum estradiol increased to a very high level within 1 week. These data implicate that low-dose EMP can suppress quickly and adequately the pituitary-gonadal axis, although the antitumor effect has not as yet been elucidated. For these reasons, it is necessary to re-evaluate low-dose EMP monotherapy in previously untreated advanced prostate cancer.

Modeling Storm Surges Using Discontinuous Galerkin Methods

DTIC Science & Technology

2016-06-01

devastating impact on coastlines throughout the United States. In order to accurately understand the impacts of storm surges there needs to be an effective ...model. One of the governing systems of equations used to model storm surges’ effects is the Shallow Water Equations (SWE). In this thesis, we solve the...closer to the shoreline. In our simulation, we also learned of the effects spurious waves can have on the results. Due to boundary conditions, a

Active Control of Surge in Compressors Which Exhibit Abrupt Stall

DTIC Science & Technology

2001-06-01

sensor (of pressure, flow rate, etc.) is fed to a controller which applies a proper control law to drive the actuator (valve, The present paper reports...1993), who analyzed the influence of sensor and numerical simulation shows that: t) the predictions of control acutrsltin o th mxmm sabizd opesr...a sensor of compressor face total pressure), a The present paper considers the active suppression of surge in a butterfly throttle/actuation valve

Endothelial cell-derived microparticles loaded with iron oxide nanoparticles: feasibility of MR imaging monitoring in mice.

PubMed

Al Faraj, Achraf; Gazeau, Florence; Wilhelm, Claire; Devue, Cécile; Guérin, Coralie L; Péchoux, Christine; Paradis, Valérie; Clément, Olivier; Boulanger, Chantal M; Rautou, Pierre-Emmanuel

2012-04-01

To assess the feasibility of loading iron oxide nanoparticles in endothelial microparticles (EMPs), thereby enabling their noninvasive monitoring with magnetic resonance (MR) imaging in mice. Experiments were approved by the French Ministry of Agriculture. Endothelial cells, first labeled with anionic superparamagnetic nanoparticles, were stimulated to generate EMPs, carrying the nanoparticles in their inner compartment. C57BL/6 mice received an intravenous injection of nanoparticle-loaded EMPs, free nanoparticles, or the supernatant of nanoparticle-loaded EMPs. A 1-week follow-up was performed with a 4.7-T MR imaging device by using a gradient-echo sequence for imaging spleen, liver, and kidney and a radial very-short-echo time sequence for lung imaging. Comparisons were performed by using the Student t test. The signal intensity loss induced by nanoparticle-loaded EMPs or free nanoparticles was readily detected within 5 minutes after injection in the liver and spleen, with a more pronounced effect in the spleen for the magnetic EMPs. The kinetics of signal intensity attenuation differed for nanoparticle-loaded EMPs and free nanoparticles. No signal intensity changes were observed in mice injected with the supernatant of nanoparticle-loaded EMPs, confirming that cells had not released free nanoparticles, but only in association with EMPs. The results were confirmed by using Perls staining and immunofluorescence analysis. The strategy to generate EMPs with magnetic properties allowed noninvasive MR imaging assessment and follow-up of EMPs and opens perspectives for imaging the implications of these cellular vectors in diseases. © RSNA, 2012.

Catastrophe loss modelling of storm-surge flood risk in eastern England.

PubMed

Muir Wood, Robert; Drayton, Michael; Berger, Agnete; Burgess, Paul; Wright, Tom

2005-06-15

Probabilistic catastrophe loss modelling techniques, comprising a large stochastic set of potential storm-surge flood events, each assigned an annual rate of occurrence, have been employed for quantifying risk in the coastal flood plain of eastern England. Based on the tracks of the causative extratropical cyclones, historical storm-surge events are categorized into three classes, with distinct windfields and surge geographies. Extreme combinations of "tide with surge" are then generated for an extreme value distribution developed for each class. Fragility curves are used to determine the probability and magnitude of breaching relative to water levels and wave action for each section of sea defence. Based on the time-history of water levels in the surge, and the simulated configuration of breaching, flow is time-stepped through the defences and propagated into the flood plain using a 50 m horizontal-resolution digital elevation model. Based on the values and locations of the building stock in the flood plain, losses are calculated using vulnerability functions linking flood depth and flood velocity to measures of property loss. The outputs from this model for a UK insurance industry portfolio include "loss exceedence probabilities" as well as "average annualized losses", which can be employed for calculating coastal flood risk premiums in each postcode.

Towards a full representation of tropical cyclones in a global reanalysis of extreme sea levels

NASA Astrophysics Data System (ADS)

Muis, S.; Verlaan, M.; Lin, N.; Winsemius, H.; Vatvani, D.; Ward, P.; Aerts, J.

2016-12-01

Tropical cyclones (TCs), including hurricanes and typhoons, are characterised by high wind speeds and low pressure, and cause dangerous storm surges in coastal areas. Recent disasters like the flooding of New Orleans in 2005 due to Hurricane Katrina and of New York in 2012 due to Hurricane Sandy exemplify the significant TC risk in the United States. In this contribution, we present a new framework to model TC storm surges and probabilities at the Atlantic basin- and, ultimately, global scales. This works builds on the work of Muis et al. (2016), which presented the first dynamically-derived reanalysis dataset of storm surges that covers the entire world's coastline (GTSR dataset). Surge levels for the period 1979-2014 were simulated by forcing the Global Surge and Tide Model (GTSM) with wind speed and atmospheric pressure from the ERA-Interim reanalysis. There is generally a good agreement between simulated and observed sea level extremes in extra-tropical regions; however for areas prone to TCs there is a severe underestimation of extremes. For example, the maximum surge levels during Hurricane Katrina in New Orleans exceeded 8 m, whilst the GTSM surge levels in that area do not exceed 2-3 m. Hence, due to the coarse grid resolution, the strong intensities of TCs are not fully captured in ERA-Interim. Furthermore, the length of ERA-Interim data set, like other reanalysis datasets, is too short to estimate the probabilities of extreme TC events in a reliable way. For accurate risk assessments it is essential to improve the representation of TCs in these global reanalysis of extreme sea levels. First, we need a higher resolution of meteorological forcing, which can be modelled with input from the observed best track data. Second, we need to statistically extend the observed record to many thousands of years. We will present the first results of these steps for the east coast of the United States. We will validate the GTSM model forced with best track data using recent extreme events like Katrina and Sandy. We will investigate how the statistics of the extreme sea level will change due to improved representation of TCs.

Developing an early warning system for storm surge inundation in the Philippines

NASA Astrophysics Data System (ADS)

Tablazon, Judd; Mahar Francisco Lagmay, Alfredo; Francia Mungcal, Ma. Theresa; Gonzalo, Lia Anne; Dasallas, Lea; Briones, Jo Brianne Louise; Santiago, Joy; Suarez, John Kenneth; Lapidez, John Phillip; Caro, Carl Vincent; Ladiero, Christine; Malano, Vicente

2014-05-01

A storm surge is the sudden rise of sea water generated by an approaching storm, over and above the astronomical tides. This event imposes a major threat in the Philippine coastal areas, as manifested by Typhoon Haiyan on 08 November 2013 where more than 6,000 people lost their lives. It has become evident that the need to develop an early warning system for storm surges is of utmost importance. To provide forecasts of the possible storm surge heights of an approaching typhoon, the Nationwide Operational Assessment of Hazards under the Department of Science and Technology (DOST-Project NOAH) simulated historical tropical cyclones that entered the Philippine Area of Responsibility. Bathymetric data, storm track, central atmospheric pressure, and maximum wind speed were used as parameters for the Japan Meteorological Agency (JMA) Storm Surge Model. The researchers calculated the frequency distribution of maximum storm surge heights of all typhoons under a specific Public Storm Warning Signal (PSWS) that passed through a particular coastal area. This determines the storm surge height corresponding to a given probability of occurrence. The storm surge heights from the model were added to the maximum astronomical tide data from WXTide software. The team then created maps of probable area inundation and flood levels of storm surges along coastal areas for a specific PSWS using the results of the frequency distribution. These maps were developed from the time series data of the storm tide at 10-minute intervals of all observation points in the Philippines. This information will be beneficial in developing early warnings systems, static maps, disaster mitigation and preparedness plans, vulnerability assessments, risk-sensitive land use plans, shoreline defense efforts, and coastal protection measures. Moreover, these will support the local government units' mandate to raise public awareness, disseminate information about storm surge hazards, and implement appropriate counter-measures for a given PSWS.

Developing an early warning system for storm surge inundation in the Philippines

NASA Astrophysics Data System (ADS)

Tablazon, J.; Caro, C. V.; Lagmay, A. M. F.; Briones, J. B. L.; Dasallas, L.; Lapidez, J. P.; Santiago, J.; Suarez, J. K.; Ladiero, C.; Gonzalo, L. A.; Mungcal, M. T. F.; Malano, V.

2014-10-01

A storm surge is the sudden rise of sea water generated by an approaching storm, over and above the astronomical tides. This event imposes a major threat in the Philippine coastal areas, as manifested by Typhoon Haiyan on 8 November 2013 where more than 6000 people lost their lives. It has become evident that the need to develop an early warning system for storm surges is of utmost importance. To provide forecasts of the possible storm surge heights of an approaching typhoon, the Nationwide Operational Assessment of Hazards under the Department of Science and Technology (DOST-Project NOAH) simulated historical tropical cyclones that entered the Philippine Area of Responsibility. Bathymetric data, storm track, central atmospheric pressure, and maximum wind speed were used as parameters for the Japan Meteorological Agency Storm Surge Model. The researchers calculated the frequency distribution of maximum storm surge heights of all typhoons under a specific Public Storm Warning Signal (PSWS) that passed through a particular coastal area. This determines the storm surge height corresponding to a given probability of occurrence. The storm surge heights from the model were added to the maximum astronomical tide data from WXTide software. The team then created maps of probable area inundation and flood levels of storm surges along coastal areas for a specific PSWS using the results of the frequency distribution. These maps were developed from the time series data of the storm tide at 10 min intervals of all observation points in the Philippines. This information will be beneficial in developing early warnings systems, static maps, disaster mitigation and preparedness plans, vulnerability assessments, risk-sensitive land use plans, shoreline defense efforts, and coastal protection measures. Moreover, these will support the local government units' mandate to raise public awareness, disseminate information about storm surge hazards, and implement appropriate counter-measures for a given PSWS.

A numerical study on hurricane-induced storm surge and inundation in Charleston Harbor, South Carolina

NASA Astrophysics Data System (ADS)

Peng, Machuan; Xie, Lian; Pietrafesa, Leonard J.

2006-08-01

A storm surge and inundation model is configured in Charleston Harbor and its adjacent coastal region to study the harbor's response to hurricanes. The hydrodynamic component of the modeling system is based on the Princeton Ocean Model, and a scheme with multiple inundation speed options is imbedded in the model for the inundation calculation. Historic observations (Hurricane Hugo and its related storm surge and inundation) in the Charleston Harbor region indicate that among three possible inundation speeds in the model, taking Ct (gd)1/2 (Ct is a terrain-related parameter) as the inundation speed is the best choice. Choosing a different inundation speed in the model has effects not only on inundation area but also on storm surge height. A nesting technique is necessary for the model system to capture the mesoscale feature of a hurricane and meanwhile to maintain a higher horizontal resolution in the harbor region, where details of the storm surge and inundation are required. Hurricane-induced storm surge and inundation are very sensitive to storm tracks. Twelve hurricanes with different tracks are simulated to investigate how Charleston Harbor might respond to tracks that are parallel or perpendicular to the coastline or landfall at Charleston at different angles. Experiments show that large differences of storm surge and inundation may have occurred if Hurricane Hugo had approached Charleston Harbor with a slightly different angle. A hurricane's central pressure, radius of maximum wind, and translation speed have their own complicated effects on surge and inundation when the hurricane approaches the coast on different tracks. Systematic experiments are performed in order to illustrate how each of such factors, or a combination of them, may affect the storm surge height and inundation area in the Charleston Harbor region. Finally, suggestions are given on how this numerical model system may be used for hurricane-induced storm surge and inundation forecasting.

Equivalent Air Spring Suspension Model for Quarter-Passive Model of Passenger Vehicles.

PubMed

Abid, Haider J; Chen, Jie; Nassar, Ameen A

2015-01-01

This paper investigates the GENSIS air spring suspension system equivalence to a passive suspension system. The SIMULINK simulation together with the OptiY optimization is used to obtain the air spring suspension model equivalent to passive suspension system, where the car body response difference from both systems with the same road profile inputs is used as the objective function for optimization (OptiY program). The parameters of air spring system such as initial pressure, volume of bag, length of surge pipe, diameter of surge pipe, and volume of reservoir are obtained from optimization. The simulation results show that the air spring suspension equivalent system can produce responses very close to the passive suspension system.

Sustained apnea induces endothelial activation.

PubMed

Eichhorn, Lars; Dolscheid-Pommerich, Ramona; Erdfelder, Felix; Ayub, Muhammad Ajmal; Schmitz, Theresa; Werner, Nikos; Jansen, Felix

2017-09-01

Apnea diving has gained worldwide popularity, even though the pathophysiological consequences of this challenging sport on the human body are poorly investigated and understood. This study aims to assess the influence of sustained apnea in healthy volunteers on circulating microparticles (MPs) and microRNAs (miRs), which are established biomarkers reflecting vascular function. Short intermittent hypoxia due to voluntary breath-holding affects circulating levels of endothelial cell-derived MPs (EMPs) and endothelial cell-derived miRs. Under dry laboratory conditions, 10 trained apneic divers performed maximal breath-hold. Venous blood samples were taken, once before and at 4 defined points in time after apnea. Samples were analyzed for circulating EMPs and endothelial miRs. Average apnea time was 329 seconds (±103), and SpO 2 at the end of apnea was 79% (±12). Apnea was associated with a time-dependent increase of circulating endothelial cell-derived EMPs and endothelial miRs. Levels of circulating EMPs in the bloodstream reached a peak 4 hours after the apnea period and returned to baseline levels after 24 hours. Circulating miR-126 levels were elevated at all time points after a single voluntary maximal apnea, whereas miR-26 levels were elevated significantly only after 30 minutes and 4 hours. Also miR-21 and miR-92 levels increased, but did not reach the level of significance. Even a single maximal breath-hold induces acute endothelial activation and should be performed with great caution by subjects with preexisting vascular diseases. Voluntary apnea might be used as a model to simulate changes in endothelial function caused by hypoxia in humans. © 2017 Wiley Periodicals, Inc.

Plasmodium falciparum erythrocyte membrane protein-1 specifically suppresses early production of host interferon-gamma.

PubMed

D'Ombrain, Marthe C; Voss, Till S; Maier, Alexander G; Pearce, J Andrew; Hansen, Diana S; Cowman, Alan F; Schofield, Louis

2007-08-16

Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is a variable antigen expressed by P. falciparum, the malarial parasite. PfEMP-1, present on the surface of infected host erythrocytes, mediates erythrocyte binding to vascular endothelium, enabling the parasite to avoid splenic clearance. In addition, PfEMP-1 is proposed to regulate host immune responses via interactions with the CD36 receptor on antigen-presenting cells. We investigated the immunoregulatory function of PfEMP-1 by comparing host cell responses to erythrocytes infected with either wild-type parasites or transgenic parasites lacking PfEMP-1. We showed that PfEMP-1 suppresses the production of the cytokine interferon-gamma by human peripheral blood mononuclear cells early after exposure to P. falciparum. Suppression of this rapid proinflammatory response was CD36 independent and specific to interferon-gamma production by gammadelta-T, NK, and alphabeta-T cells. These data demonstrate a parasite strategy for downregulating the proinflammatory interferon-gamma response and further establish transgenic parasites lacking PfEMP-1 as powerful tools for elucidating PfEMP-1 functions.

Evaluation of erythroblast macrophage protein related to erythroblastic islands in patients with hematopoietic stem cell transplantation

PubMed Central

2013-01-01

Background Hematopoietic evaluation of the patients after Hematopoietic stem cell transplantation (HSCT) is very important. Erythroblast macrophage protein (Emp) is a key protein with function in normal differentiation of erythroid cells and macrophages. Emp expression correlates with erythroblastic island formation, a process widely believed to be associated with hematopoiesis in bone marrow. We aimed to investigate the hematopoietic function of bone marrow from 46 HSCT patients and 16 inpatients with severe anemia applied to the treatment of EPO by measuring Emp expression level. Methods Emp mRNA and protein expression levels in mononuclear cells of bone marrow and peripheral blood samples were detected by RT-PCR and Western blotting method respectively. Results While hematopoiesis occurs in bone marrow, Emp expression level was elevated and more erythroblastic islands were found , and Emp is upregulated in bone marrow in response to erythropoietin (EPO) treatment. Conclusions Emp expression correlates with erythroblastic island formation and has an important function for bone marrow hematopoiesis. Emp could be a potential biomarker for hematopoietic evaluation of HSCT patients. PMID:23566571

EMP-1 promotes tumorigenesis of NSCLC through PI3K/AKT pathway.

PubMed

Lai, Senyan; Wang, Guihua; Cao, Xiaonian; Li, Zhaoming; Hu, Junbo; Wang, Jing

2012-12-01

This study examined the role of EMP-1 in tumorigenesis of non-small cell lung carcinoma (NSCLC) and the possible mechanism. Specimens were collected from 28 patients with benign lung diseases and 28 with NSCLC, and immunohistochemically detected to evaluate the correlation of EMP-1 expression to the clinical features of NSCLC. Recombinant adenovirus was constructed to over-express EMP-1 and then infect PC9 cells. Cell proliferation was measured by Ki67 staining. Western blotting was performed to examine the effect of EMP-1 on the PI3K/AKT signaling. Moreover, tumor xenografts were established by subcutaneous injection of PC9 cell suspension (about 5×10(7)/mL in 100 μL of PBS) into the right hind limbs of athymic nude mice. The results showed EMP-1 was significantly up-regulated in NSCLC patients as compared with those with benign lung diseases. Over-expression of EMP-1 promoted proliferation of PC9 cells, which coincided with the activation of the PI3K/AKT pathway. EMP-1 promoted the growth of xenografts of PC9 cells in athymic nude mice. It was concluded that EMP-1 expression may contribute to the development and progress of NSCLC by activating PI3K/AKT pathway.

Linkage of Rainfall-Runoff and Hurricane Storm Surge in Galveston Bay

NASA Astrophysics Data System (ADS)

Deitz, R.; Christian, J.; Wright, G.; Fang, N.; Bedient, P.

2012-12-01

In conjunction with the SSPEED Center, large rainfall events in the upper Gulf of Mexico are being studied in an effort to help design a surge gate to protect the Houston Ship Channel during hurricane events. The ship channel is the world's second largest petrochemical complex and the Coast Guard estimates that a one-month closure would have a $60 billion dollar impact on the national economy. In this effort, statistical design storms, such as the 24-hour PMP, as well as historical storms, like Hurricane Ike, Hurricane Katrina, and Hurricane Rita, are being simulated in a hydrologic/hydraulic model using radar and rain gauge data. VfloTM, a distributed hydrologic model, is being used to quantify the effect that storm size, intensity, and location has on timing and peak flows in the in the upper drainage area. These hydrographs were input to a hydraulic model with various storm surges from Galveston Bay. Results indicate that there is a double peak phenomenon with flows from the west draining days earlier than flows from the north. With storm surge typically lasting 36-48 hours, this indicates the flows from the west are interacting with the storm surge, whereas flows from the north would arrive once the storm surge is receding. Gate operations were optimized in the model to account for the relative timing of upland runoff and hurricane surge, and to quantify the capability of the gate structure to protect the Ship Channel industry.

[Clinical analysis on extramedullary plasmacytoma in the upper airway].

PubMed

Ge, Shenglei; Tan, Zhiqiang; Xie, Dinghua; Yi, Yan

2013-03-01

To investigate the clinical manifestation, treatment and prognosis of extramedullary plasmacytoma(EMP) in the upper airway, and to improve the diagnosis and outcome of EMP treatment. Clinical data of 26 EMP cases were reviewed retrospectively, and then compared with multiple myeloma(MM) patients presenting with lesions in upper airway. Of 26 cases, 9 cases with the tumors occurred in nasal cavities, 7 in nasal sinuses, 6 in pharyngeal, 4 in throat, mainly manifesting with local masses and relevant symptoms. The manifestations of clinical, endoscopy findings and pathologic results in EMP patients were not distinguishable from the lesions of MM patients, while MM patients often accompanied by other findings, such as anemia and bone damage. Involvement of neck lymph nodes was more common in MM patients than in EMP patients. Ten patients were treated with surgery, and 16 patients with surgery and radiotherapy. Of the seven EMP patients with involvement of neck lymph nodes, four patients received additional chemotherapy besides surgery and radiotherapy, and no local relapse and MM happened in them, while of the three patients only received surgery and radiotherapy, one local relapse were found and one progressed to MM. The diagnosis of EMPs mainly depends on pathological results. The judgment of pathologists and application of molecular biology technology are vital for the diagnosis of EMP in upper airway, and MM must be excluded very carefully in the diagnosis of EMP. Surgery combined with radiotherapy is the main treatment for EMP in the upper airway, and the prognosis is good but the follow-up should be taken. Besides surgery and radiotherapy, chemotherapy is beneficial for the EMP patients accompanied with lesions in neck lymph nodes.

A comparative assessment of adverse event classification in the out-of-hospital setting.

PubMed

Patterson, P Daniel; Lave, Judith R; Weaver, Matthew D; Guyette, Francis X; Arnold, Robert M; Martin-Gill, Christian; Rittenberger, Jon C; Krackhardt, David; Mosesso, Vincent N; Roth, Ronald N; Wadas, Richard J; Yealy, Donald M

2014-01-01

We sought to test reliability of two approaches to classify adverse events (AEs) associated with helicopter EMS (HEMS) transport. The first approach for AE classification involved flight nurses and paramedics (RN/Medics) and mid-career emergency physicians (MC-EMPs) independently reviewing 50 randomly selected HEMS medical records. The second approach involved RN/Medics and MC-EMPs meeting as a group to openly discuss 20 additional medical records and reach consensus-based AE decision. We compared all AE decisions to a reference criterion based on the decision of three senior emergency physicians (Sr-EMPs). We designed a study to detect an improvement in agreement (reliability) from fair (kappa = 0.2) to moderate (kappa = 0.5). We calculated sensitivity, specificity, percent agreement, and positive and negative predictive values (PPV/NPV). For the independent reviews, the Sr-EMP group identified 26 AEs while individual clinician reviewers identified between 19 and 50 AEs. Agreement on the presence/absence of an AE between Sr-EMPs and three MC-EMPs ranged from κ = 0.20 to κ = 0.25. Agreement between Sr-EMPs and three RN/Medics ranged from κ = 0.11 to κ = 0.19. For the consensus/open-discussion approach, the Sr-EMPs identified 13 AEs, the MC-EMP group identified 18 AEs, and RN/medic group identified 36 AEs. Agreement between Sr-EMPs and MC-EMP group was (κ = 0.30 95%CI -0.12, 0.72), whereas agreement between Sr-EMPs and RN/medic group was (κ = 0.40 95%CI 0.01, 0.79). Agreement between all three groups was fair (κ = 0.33, 95%CI 0.06, 0.66). Percent agreement (58-68%) and NPV (63-76%) was moderately dissimilar between clinicians, while sensitivity (25-80%), specificity (43-97%), and PPV (48-83%) varied. We identified a higher level of agreement/reliability in AE decisions utilizing a consensus-based approach for review rather than independent reviews.

A retrospective study of correlation of morphologic patterns, MIB1 proliferation index, and survival analysis in 134 cases of plasmacytoma.

PubMed

Ghodke, Kiran; Shet, Tanuja; Epari, Sridhar; Sengar, Manju; Menon, Hari; Gujral, Sumeet

2015-06-01

Plasmacytoma classified into solitary plasmacytoma of bone (SPB) and extramedullary plasmacytoma (EMP) is characterized by infiltrate of plasma cells of diverse maturity and by their monoclonal immunoglobulin products. Both SPB and EMP represent different groups of neoplasm in terms of location, tumor progression, and overall survival rate. There is a need for features that indicate likelihood of myeloma in patients with plasmacytoma without other manifestations. This study was an attempt to study the morphologic patterns of plasmacytoma (SPB and EMP), MIB1 proliferation index, and correlation of these with clinicopathologic features and survival of the patients. The study group comprised of 134 cases of plasmacytoma (88 SPB and 46 EMP) over duration of 8 years and were graded as per Bartl's histologic grading system. Commonest site was vertebral body in SPB (36%) and upper aerodigestive tract in EMP (48%). On serum electrophoresis, overall M band was detected in 41% cases. Both SPB and EMP on histology revealed similar morphologic features. MIB1 proliferation index ranged from less than 1% to 80%. It was slightly higher in EMP in comparison with SPB (P value = .002). Seventy percent of cases, which progressed to multiple myeloma (MM) showed MIB1 labeling index more than 10%; however, it was not statistically significant in predicting the disease progression. With the median follow-up of 19 months (range, 1-99 months), 10 SPB had disease progression of which 7 converted to MM, and 3 developed EMP, with a median interval of 21 months (range, 8-75 months) for the development of MM and 3 months (range, 3-9 months) for the progression to EMP. Five-year survival for EMP varied by site, with poorest survival in brain/central nervous system EMP as compared with EMP at other sites. To conclude, grade and MIB1 proliferation index help in predicting aggressive course in plasmacytoma. Copyright © 2015 Elsevier Inc. All rights reserved.

(R)-[¹¹C]Emopamil as a novel tracer for imaging enhanced P-glycoprotein function.

PubMed

Toyohara, Jun; Okamoto, Mayumi; Aramaki, Hiroki; Zaitsu, Yuto; Shimizu, Isao; Ishiwata, Kiichi

2016-01-01

2-Isopropyl-5-[methyl-(2-phenylethyl)amino]-2-phenylpentanenitrile (emopamil; EMP) is a calcium channel blocker of the phenylalkylamine class, with weak substrate properties for P-glycoprotein (P-gp). A weak substrate for P-gp would be suitable for measuring enhanced P-gp function. This study was performed to synthesise (R)- and (S)-[(11)C]EMP and characterise their properties as P-gp tracers. We synthesised (R)- and (S)-[(11)C]EMP and compared their biodistribution, peripheral metabolism, and effects of the P-gp inhibitor cyclosporine A (CsA, 50 mg/kg). We compared the brain pharmacokinetics of (R)-[(11)C]EMP and (R)-[(11)C]verapamil [(R)-[(11)C]VER] at baseline and CsA pretreatment with small animal positron emission tomography (PET). (R)- and (S)-[(11)C]EMP were synthesised from (R)- and (S)-noremopamil, respectively, by methylation with [(11)C]methyl triflate in the presence of NaOH at room temperature. (R)- and (S)-[(11)C]EMP yields were ~30%, with specific activity>74 GBq/μmol and radiochemical purity>99%. (R)-[(11)C]EMP showed significantly greater uptake in the mouse brain than (S)-[(11)C]EMP. Both showed homogeneous non-stereoselective regional brain distributions. (R)- and (S)-[(11)C]EMP were rapidly metabolised to hydrophilic metabolites. Unchanged plasma (S)-[(11)C]EMP level was significantly lower than that of (R)-[(11)C]EMP 15 minutes post-injection, whilst>88% of radioactivity in the brain was intact at 15 minutes post-injection and was non-stereoselective. CsA pretreatment increased brain activity ~3-fold in mice, but was non-stereoselective. The baseline area-under-the-curve (AUC) of brain radioactivity (0-60 minutes) of (R)-[(11)C]EMP was 2-fold higher than that of (R)-[(11)C]VER, but their AUCs after CsA pretreatment were comparable. (R)-[(11)C]EMP is a novel tracer for imaging P-gp function with higher baseline uptake than (R)-[(11)C]VER. Copyright © 2015 Elsevier Inc. All rights reserved.

Annual FEV1 changes and numbers of circulating endothelial microparticles in patients with COPD: a prospective study

PubMed Central

Takahashi, Toru; Kobayashi, Seiichi; Fujino, Naoya; Suzuki, Takaya; Ota, Chiharu; Tando, Yukiko; Yamada, Mitsuhiro; Yanai, Masaru; Yamaya, Mutsuo; Kurosawa, Shin; Yamauchi, Masanori; Kubo, Hiroshi

2014-01-01

Objective Growing evidence suggests that endothelial injury is involved in the pathophysiology of chronic obstructive pulmonary disease (COPD). Circulating endothelial microparticles (EMPs) increase in patients with COPD because of the presence of endothelial injury. We examined the relationship between EMP number and changes in forced expiratory volume in 1 s (FEV1) in patients with COPD. Design Prospective study. Setting One hospital in Japan. Participants A total 48 outpatients with stable COPD coming to the hospital from September 2010 to September 2011. Primary and secondary outcomes measured Blood samples were collected and vascular endothelial (VE)-cadherin EMPs (CD144+ EMPs), E-selectin EMPs (CD62E+ EMPs) and platelet endothelial cell adhesion molecule EMPs (CD31+/CD41− EMPs) were measured using fluorescence-activated cell sorting. Annual FEV1 changes were evaluated using FEV1 data acquired a year before and a year after sample collection. Results The number of E-selectin and VE-cadherin EMPs showed significant negative correlations with annual FEV1 changes (rs=−0.65, p<0.001, rs=−0.43, p=0.003, respectively). Leucocyte counts tended to be correlated with annual FEV1 changes, but this correlation was not significant (rs=−0.28, p=0.057). There were significant differences in annual FEV1 changes between with and without history of frequent exacerbation (p=0.006), and among Global Initiative for Chronic Obstructive Lung Disease (GOLD) stages (p=0.009). Multiple linear regression analysis revealed E-selectin EMP to be the only significant parameter associated with annual FEV1 changes, independent of VE-cadherin EMP, GOLD stages, leucocyte counts, and history of frequent exacerbation. Receiver operating characteristic curves showed the optimum E-selectin EMP cut-off level for prediction of rapid FEV1 decline (>66 mL/year) to be 153.0/µL (areas under curve 0.78 (95% CI 0.60 to 0.89); sensitivity, 67%; specificity, 81%). Conclusions The high E-selectin EMP levels in stable patients with COPD are predictive of rapid FEV1 decline. Trial registration number UMIN000005168. PMID:24604485

Water Hammer Simulations of Monomethylhydrazine Propellant

NASA Technical Reports Server (NTRS)

Burkhardt, Zachary; Ramachandran, N.; Majumdar, A.

2017-01-01

Fluid Transient analysis is important for the design of spacecraft propulsion system to ensure structural stability of the system in the event of sudden closing or opening of the valve. Generalized Fluid System Simulation Program (GFSSP), a general purpose flow network code developed at NASA/MSFC is capable of simulating pressure surge due to sudden opening or closing of valve when thermodynamic properties of real fluid are available for the entire range of simulation. Specifically GFSSP needs an accurate representation of pressure density relationship in order to predict pressure surge during a fluid transient. Unfortunately, the available thermodynamic property programs such as REFPROP, GASP or GASPAK do not provide the thermodynamic properties of Monomethylhydrazine(MMH). This work illustrates the process used for building a customized table of properties of state variables from available properties and speed of sound that is required by GFSSP for simulation. Good agreement was found between the simulations and measured data. This method can be adopted for modeling flow networks and systems with other fluids whose properties are not known in detail in order to obtain general technical insight.

Application of a coupled vegetation competition and groundwater simulation model to study effects of sea level rise and storm surges on coastal vegetation

USGS Publications Warehouse

Teh, Su Yean; Turtora, Michael; DeAngelis, Donald L.; Jiang Jiang,; Pearlstine, Leonard G.; Smith, Thomas; Koh, Hock Lye

2015-01-01

Global climate change poses challenges to areas such as low-lying coastal zones, where sea level rise (SLR) and storm-surge overwash events can have long-term effects on vegetation and on soil and groundwater salinities, posing risks of habitat loss critical to native species. An early warning system is urgently needed to predict and prepare for the consequences of these climate-related impacts on both the short-term dynamics of salinity in the soil and groundwater and the long-term effects on vegetation. For this purpose, the U.S. Geological Survey’s spatially explicit model of vegetation community dynamics along coastal salinity gradients (MANHAM) is integrated into the USGS groundwater model (SUTRA) to create a coupled hydrology–salinity–vegetation model, MANTRA. In MANTRA, the uptake of water by plants is modeled as a fluid mass sink term. Groundwater salinity, water saturation and vegetation biomass determine the water available for plant transpiration. Formulations and assumptions used in the coupled model are presented. MANTRA is calibrated with salinity data and vegetation pattern for a coastal area of Florida Everglades vulnerable to storm surges. A possible regime shift at that site is investigated by simulating the vegetation responses to climate variability and disturbances, including SLR and storm surges based on empirical information.

Use of Lean Response to Improve Pandemic Influenza Surge in Public Health Laboratories

PubMed Central

Chang, Yin; Prystajecky, Natalie; Petric, Martin; Mak, Annie; Abbott, Brendan; Paris, Benjamin; Decker, K.C.; Pittenger, Lauren; Guercio, Steven; Stott, Jeff; Miller, Joseph D.

2012-01-01

A novel influenza A (H1N1) virus detected in April 2009 rapidly spread around the world. North American provincial and state laboratories have well-defined roles and responsibilities, including providing accurate, timely test results for patients and information for regional public health and other decision makers. We used the multidisciplinary response and rapid implementation of process changes based on Lean methods at the provincial public health laboratory in British Columbia, Canada, to improve laboratory surge capacity in the 2009 influenza pandemic. Observed and computer simulating evaluation results from rapid processes changes showed that use of Lean tools successfully expanded surge capacity, which enabled response to the 10-fold increase in testing demands. PMID:22257385

Contrasting Hydrodynamic and Environmental Effects of Hurricanes Harvey and Ike in a Highly Industrialized Estuary

NASA Astrophysics Data System (ADS)

Kiaghadi, A.; Rifai, H. S.

2017-12-01

It is commonly believed that storm surge is the most destructive aspect of hurricanes. However, massive rainfall with a return period of 100 years or more induced by hurricanes can cause more catastrophic damage than losses caused by storm surge as demonstrated recently by hurricanes Harvey, Irma and Maria. In this study the hydrodynamics and environmental effects of hurricanes Ike and Harvey were compared and contrasted by linking hydrodynamic flow models with water quality models to simulate spills from storage tanks located in the Houston Ship Channel (HSC). Hurricane Ike with a maximum surge of 5.3 meters in Galveston Bay and Harvey with a maximum rainfall of 1.25 meters both struck the HSC region in Texas in 2008 and 2017, respectively. Both events resulted in numerous spills from municipal and industrial facilities, hazardous waste sites, superfund sites, and landfills. The Environmental Fluid Dynamic Code (EFDC) was coupled with the SWAN+ADCIRC hurricane simulation model to simulate Hurricane Ike and EFDC was coupled with USGS flow boundary conditions to model Hurricane Harvey. A conservative dye release was used to simulate a chemical release during each event. The results showed Hurricane Harvey caused higher water surface elevations within the HSC accompanied by longer and wider-spread land inundation. In contrast, higher water surface elevations were observed within the shallow side bays during Hurricane Ike that caused sediment resuspension and repartitioning of pollutants. Rapid spill mass transportation was observed for both hurricanes; 50% of total spill mass reached Galveston Bay in 20 and 22 hours after a spill event for Hurricane Harvey and Ike, respectively, and more than 90% of the spill mass reached the bay in 36 and 48 hours, respectively. Unlike Hurricane Harvey, the conservative tracer was spread almost 2.5 km upstream of the releasing point for Hurricane Ike due to surge. However, during Harvey, 35% more land was affected by the spilled mass with five times more remained mass on the land.

Numerical investigation of rotating stall in centrifugal compressor with vaned and vaneless diffuser

NASA Astrophysics Data System (ADS)

Halawa, Taher; Alqaradawi, Mohamed; Gadala, Mohamed S.; Shahin, Ibrahim; Badr, Osama

2015-06-01

This study presents a numerical simulation of the stall and surge in a centrifugal compressor and presents a descriptionof the stall development in two different cases. The first case is for a compressor with vaneless diffuser and the second is for a compressor with vaned diffuser of the vane island shape. The main aim of this study is to compare the flow characteristics and behavior for the two compressors near the surge operating condition and provide further understanding of the diffuser role when back flow occurs at surge. Results showed that for a locationnear the diffuser entrance, the amplitude of the static pressure fluctuations for the vaneless diffuser case is higher than that for the vaned diffuser case near surge condition. These pressure fluctuations in the case of the vaneless diffuser appear with a gradual decrease of the mean pressure value as a part of the surge cycle. While for the case of the vaned diffuser, the pressure drop during surge occurs faster than the case of the vaneless diffuser. Also, results indicated that during surge in the case of vaneless diffuser, there is a region with low velocity and back flow that appears as a layer connecting all impeller passages near shroud surface and this layer develops in size with time. On the other hand, for the case of vaned diffuser during surge, the low velocity regions appear in random locations in some passages and these regions expand with time towards the shroud surface. Results showed that during stall, the impeller passages are exposed to identical impact from stall cells in the case of vaneless diffuser while the stall effect varies from passage to another in the case of the vaned diffuser.

Simulating the roles of crevasse routing of surface water and basal friction on the surge evolution of Basin 3, Austfonna ice cap

NASA Astrophysics Data System (ADS)

Gong, Yongmei; Zwinger, Thomas; Åström, Jan; Altena, Bas; Schellenberger, Thomas; Gladstone, Rupert; Moore, John C.

2018-05-01

The marine-terminating outlet in Basin 3, Austfonna ice cap, has been accelerating since the mid-1990s. Stepwise multi-annual acceleration associated with seasonal summer speed-up events was observed before the outlet entered the basin-wide surge in autumn 2012. We used multiple numerical models to explore hydrologic activation mechanisms for the surge behaviour. A continuum ice dynamic model was used to invert basal friction coefficient distributions using the control method and observed surface velocity data between April 2012 and July 2014. This has provided input to a discrete element model capable of simulating individual crevasses, with the aim of finding locations where meltwater entered the glacier during the summer and reached the bed. The possible flow paths of surface meltwater reaching the glacier bed as well as those of meltwater produced at the bed were calculated according to the gradient of the hydraulic potential. The inverted friction coefficients show the unplugging of the stagnant ice front and expansion of low-friction regions before the surge reached its peak velocity in January 2013. Crevasse distribution reflects the basal friction pattern to a high degree. The meltwater reaches the bed through the crevasses located above the margins of the subglacial valley and the basal melt that is generated mainly by frictional heating flows either to the fast-flowing units or potentially accumulates in an overdeepened region. Based on these results, the mechanisms facilitated by basal meltwater production, crevasse opening and the routing of meltwater to the bed are discussed for the surge in Basin 3.

Evaluation of the HadGEM3-A simulations in view of detection and attribution of human influence on extreme events in Europe

NASA Astrophysics Data System (ADS)

Vautard, Robert; Christidis, Nikolaos; Ciavarella, Andrew; Alvarez-Castro, Carmen; Bellprat, Omar; Christiansen, Bo; Colfescu, Ioana; Cowan, Tim; Doblas-Reyes, Francisco; Eden, Jonathan; Hauser, Mathias; Hegerl, Gabriele; Hempelmann, Nils; Klehmet, Katharina; Lott, Fraser; Nangini, Cathy; Orth, René; Radanovics, Sabine; Seneviratne, Sonia I.; van Oldenborgh, Geert Jan; Stott, Peter; Tett, Simon; Wilcox, Laura; Yiou, Pascal

2018-04-01

A detailed analysis is carried out to assess the HadGEM3-A global atmospheric model skill in simulating extreme temperatures, precipitation and storm surges in Europe in the view of their attribution to human influence. The analysis is performed based on an ensemble of 15 atmospheric simulations forced with observed sea surface temperature of the 54 year period 1960-2013. These simulations, together with dual simulations without human influence in the forcing, are intended to be used in weather and climate event attribution. The analysis investigates the main processes leading to extreme events, including atmospheric circulation patterns, their links with temperature extremes, land-atmosphere and troposphere-stratosphere interactions. It also compares observed and simulated variability, trends and generalized extreme value theory parameters for temperature and precipitation. One of the most striking findings is the ability of the model to capture North-Atlantic atmospheric weather regimes as obtained from a cluster analysis of sea level pressure fields. The model also reproduces the main observed weather patterns responsible for temperature and precipitation extreme events. However, biases are found in many physical processes. Slightly excessive drying may be the cause of an overestimated summer interannual variability and too intense heat waves, especially in central/northern Europe. However, this does not seem to hinder proper simulation of summer temperature trends. Cold extremes appear well simulated, as well as the underlying blocking frequency and stratosphere-troposphere interactions. Extreme precipitation amounts are overestimated and too variable. The atmospheric conditions leading to storm surges were also examined in the Baltics region. There, simulated weather conditions appear not to be leading to strong enough storm surges, but winds were found in very good agreement with reanalyses. The performance in reproducing atmospheric weather patterns indicates that biases mainly originate from local and regional physical processes. This makes local bias adjustment meaningful for climate change attribution.

Process-based model predictions of hurricane induced morphodynamic change on low-lying barrier islands

USGS Publications Warehouse

Plant, Nathaniel G.; Thompson, David M.; Elias, Edwin; Wang, Ping; Rosati, Julie D.; Roberts, Tiffany M.

2011-01-01

Using Delft3D, a Chandeleur Island model was constructed to examine the sediment-transport patterns and morphodynamic change caused by Hurricane Katrina and similar storm events. The model setup included a coarse Gulf of Mexico domain and a nested finer-resolution Chandeleur Island domain. The finer-resolution domain resolved morphodynamic processes driven by storms and tides. A sensitivity analysis of the simulated morphodynamic response was performed to investigate the effects of variations in surge levels. The Chandeleur morphodynamic model reproduced several important features that matched observed morphodynamic changes. A simulation of bathymetric change driven by storm surge alone (no waves) along the central portion of the Chandeleur Islands showed (1) a general landward retreat and lowering of the island chain and (2) multiple breaches that increased the degree of island dissection. The locations of many of the breaches correspond with the low-lying or narrow sections of the initial bathymetry. The major part of the morphological change occurred prior to the peak of the surge when overtopping of the islands produced a strong water-level gradient and induced significant flow velocities.

Reassessing Storm Surge Risk for New York City (Invited)

NASA Astrophysics Data System (ADS)

Lin, N.; Emanuel, K.

2013-12-01

New York City (NYC) is highly vulnerable to tropical cyclone (TC) storm surge flooding. In a previous study, we coupled a (reanalysis- or GCM-driven) hurricane model with hydrodynamic models to simulate large numbers of synthetic surge events under observed and projected climates and assess surge threat for NYC. The storm surge return levels under the current and future climates (IPCC AR4 A1B scenario) were obtained. The results showed that the distribution of surge levels may shift to higher values in the future by a magnitude comparable to the projected sea-level rise. The study focused on typical TCs that have a storm size of the climatological mean for the Atlantic Basin and pass within a 200-km radius of the Battery, NYC. In October 2012, Hurricane Sandy, a barely Category-1 storm that made landfall about 200-km southwest from the Battery, caused the highest surge flooding of the instrumental record (~3.5 m above the mean sea level or ~2.8 m surge over the high tide) at the Battery. The extreme surge was due to the fact that the storm was a 'hybrid' event, undergoing extensive extratropical transition when making landfall almost perpendicularly to the NJ coast with an unusually large size. Sandy's case calls for a reassessment of storm surge risk for NYC that account for the special features of the storms in this region. In this reassessment, we account for the effect of extratropical transition on the wind fields through improving the surface background wind estimation, which was assumed to be uniform for typical TCs, by developing a representation of the interaction between the highly localized potential vorticity anomaly of the TC and its environmental baroclinic fields. We account for the storm size variation through incorporating the full probability distribution of the size for the region. Our preliminary results show that estimated wind and surge return levels are much higher with the effect of extratropical transition. The effect of the storm size variation is relatively large in the upper tail of the surge distribution. Also, we will update the prediction for future climates using the IPCC AR5 RCP 8.5 and RCP 4.5 scenarios, and extend our focus area further south to capture storms that can induce high surges at the Battery, although making landfall relatively further away on the NJ coast. The results will be compared with those using the AR4 scenario in our previous study. The combined effects of storm climatology change and sea level rise on the risk of NYC surge flooding will be discussed.

Network Flow Simulation of Fluid Transients in Rocket Propulsion Systems

NASA Technical Reports Server (NTRS)

Bandyopadhyay, Alak; Hamill, Brian; Ramachandran, Narayanan; Majumdar, Alok

2011-01-01

Fluid transients, also known as water hammer, can have a significant impact on the design and operation of both spacecraft and launch vehicle propulsion systems. These transients often occur at system activation and shutdown. The pressure rise due to sudden opening and closing of valves of propulsion feed lines can cause serious damage during activation and shutdown of propulsion systems. During activation (valve opening) and shutdown (valve closing), pressure surges must be predicted accurately to ensure structural integrity of the propulsion system fluid network. In the current work, a network flow simulation software (Generalized Fluid System Simulation Program) based on Finite Volume Method has been used to predict the pressure surges in the feed line due to both valve closing and valve opening using two separate geometrical configurations. The valve opening pressure surge results are compared with experimental data available in the literature and the numerical results compared very well within reasonable accuracy (< 5%) for a wide range of inlet-to-initial pressure ratios. A Fast Fourier Transform is preformed on the pressure oscillations to predict the various modal frequencies of the pressure wave. The shutdown problem, i.e. valve closing problem, the simulation results are compared with the results of Method of Characteristics. Most rocket engines experience a longitudinal acceleration, known as "pogo" during the later stage of engine burn. In the shutdown example problem, an accumulator has been used in the feed system to demonstrate the "pogo" mitigation effects in the feed system of propellant. The simulation results using GFSSP compared very well with the results of Method of Characteristics.

[Endothelial microparticles (EMP) in physiology and pathology].

PubMed

Sierko, Ewa; Sokół, Monika; Wojtukiewicz, Marek Z

2015-08-18

Endothelial microparticles (EMP) are released from endothelial cells (ECs) in the process of activation and/or apoptosis. They harbor adhesive molecules, enzymes, receptors and cytoplasmic structures and express a wide range of various constitutive antigens, typical for ECs, at their surface. Under physiological conditions the concentration of EMP in the blood is clinically insignificant. However, it was reported that under pathological conditions EMP concentration in the blood might slightly increase and contribute to blood coagulation, angiogenesis and inflammation. It has been shown that EMP directly and indirectly contribute to the activation of blood coagulation. Endothelial microparticles directly participate in blood coagulation through their surface tissue factor (TF) - a major initiator of blood coagulation. Furthermore, EMP exhibit procoagulant potential via expression of negatively charged phospholipids at their surface, which may promote assembly of coagulation enzymes (TF/VII, tenases and prothrombinase complexes), leading to thrombus formation. In addition, they provide a binding surface for coagulation factors: IXa, VIII, Va and IIa. Moreover, it is possible that EMP transfer TF from TF-bearing EMP to activated platelets and monocytes by binding them through adhesion molecules. Also, EMP express von Willebrand factor, which may facilitate platelet aggregation. Apart from their procoagulant properties, it was demonstrated that EMP may express adhesive molecules and metalloproteinases (MMP-2, MMP-9) at their surface and release growth factors, which may contribute to angiogenesis. Additionally, surface presence of C3 and C4 - components of the classical pathway - suggests pro-inflammatory properties of these structures. This article contains a summary of available data on the biology and pathophysiology of endothelial microparticles and their potential role in blood coagulation, angiogenesis and inflammation.

Absence of erythroblast macrophage protein (Emp) leads to failure of erythroblast nuclear extrusion.

PubMed

Soni, Shivani; Bala, Shashi; Gwynn, Babette; Sahr, Kenneth E; Peters, Luanne L; Hanspal, Manjit

2006-07-21

In mammals, the functional unit for definitive erythropoiesis is the erythroblastic island, a multicellular structure composed of a central macrophage surrounded by developing erythroblasts. Erythroblast-macrophage interactions play a central role in the terminal maturation of erythroblasts, including enucleation. One possible mediator of this cell-cell interaction is the protein Emp (erythroblast macrophage protein). We used targeted gene inactivation to define the function of Emp during hematopoiesis. Emp null embryos die perinatally and show profound alterations in the hematopoietic system. A dramatic increase in the number of nucleated, immature erythrocytes is seen in the peripheral blood of Emp null fetuses. In the fetal liver virtually no erythroblastic islands are observed, and the number of F4/80-positive macrophages is substantially reduced. Those present lack cytoplasmic projections and are unable to interact with erythroblasts. Interestingly, wild type macrophages can bind Emp-deficient erythroblasts, but these erythroblasts do not extrude their nuclei, suggesting that Emp impacts enucleation in a cell autonomous fashion. Previous studies have implicated the actin cytoskeleton and its reorganization in both erythroblast enucleation as well as in macrophage development. We demonstrate that Emp associates with F-actin and that this interaction is important in the normal distribution of F-actin in both erythroblasts and macrophages. Thus, Emp appears to be required for erythroblast enucleation and in the development of the mature macrophages. The availability of an Emp null model provides a unique experimental system to study the enucleation process and to evaluate the function of macrophages in definitive erythropoiesis.

Parallel Computation of Ocean-Atmosphere-Wave Coupled Storm Surge Model

NASA Astrophysics Data System (ADS)

Kim, K.; Yamashita, T.

2003-12-01

Ocean-atmosphere interactions are very important in the formation and development of tropical storms. These interactions are dominant in exchanging heat, momentum, and moisture fluxes. Heat flux is usually computed using a bulk equation. In this equation air-sea interface supplies heat energy to the atmosphere and to the storm. Dynamical interaction is most often one way in which it is the atmosphere that drives the ocean. The winds transfer momentum to both ocean surface waves and ocean current. The wind wave makes an important role in the exchange of the quantities of motion, heat and a substance between the atmosphere and the ocean. Storm surges can be considered as the phenomena of mean sea-level changes, which are the result of the frictional stresses of strong winds blowing toward the land and causing the set level and the low atmospheric pressure at the centre of the cyclone can additionally raise the sea level. In addition to the rise in water level itself, another wave factor must be considered. A rise of mean sea level due to white-cap wave dissipation should be considered. In bounded bodies of water, such as small seas, wind driven sea level set up is much serious than inverted barometer effects, in which the effects of wind waves on wind-driven current play an important role. It is necessary to develop the coupled system of the full spectral third-generation wind-wave model (WAM or WAVEWATCH III), the meso-scale atmosphere model (MM5) and the coastal ocean model (POM) for simulating these physical interactions. As the component of coupled system is so heavy for personal usage, the parallel computing system should be developed. In this study, first, we developed the coupling system of the atmosphere model, ocean wave model and the coastal ocean model, in the Beowulf System, for the simulation of the storm surge. It was applied to the storm surge simulation caused by Typhoon Bart (T9918) in the Yatsushiro Sea. The atmosphere model and the ocean model have been made the parallel codes by SPMD methods. The wave-current interface model was developed by defining the wave breaking stresses. And we developed the coupling program to collect and distribute the exchanging data with the parallel system. Every models and coupler are executed at same time, and they calculate own jobs and pass data with organic system. MPMD method programming was performed to couple the models. The coupler and each models united by the separated group, and they calculated by the group unit. Also they passed message when exchanging data by global unit. The data are exchanged every 60-second model time that is the least common multiple time of the atmosphere model, the wave model and the ocean model. The model was applied to the storm surge simulation in the Yatsushiro Sea, in which we could not simulated the observed maximum surge height with the numerical model that did not include the wave breaking stress. It is confirmed that the simulation which includes the wave breaking stress effects can produce the observed maximum height, 450 cm, at Matsuai.

Rationale and pre-clinical efficacy of a novel anti-EMP2 antibody for the treatment of invasive breast cancer

PubMed Central

Fu, Maoyong; Maresh, Erin L.; Helguera, Gustavo F.; Kiyohara, Meagan; Qin, Yu; Ashki, Negin; Daniels-Wells, Tracy R.; Aziz, Najib; Gordon, Lynn K.; Braun, Jonathan; Elshimali, Yahya; Soslow, Robert A.; Penichet, Manuel L.; Goodglick, Lee; Wadehra, Madhuri

2014-01-01

Despite significant advances in biology and medicine, the incidence and mortality due to breast cancer world-wide is still unacceptably high. Thus, there is an urgent need to discover new molecular targets. In this paper, we show evidence for a novel target in human breast cancer, the tetraspan protein epithelial membrane protein-2 (EMP2). Using tissue tumor arrays, protein expression of EMP2 was measured and found to be minimal in normal mammary tissue, but it was upregulated in 63% of invasive breast cancer tumors and in 73% of triple negative tumors tested. To test the hypothesis that EMP2 may be a suitable target for therapy, we constructed a fully human IgG1 antibody specific for a conserved domain of human and murine EMP2. Treatment of breast cancer cells with the anti-EMP2 IgG1 significantly inhibited EMP2 mediated signaling, blocked FAK/Src signaling, inhibited invasion, and promoted apoptosis in vitro. In both human xenograft and syngeneic metastatic tumor monotherapy models, anti-EMP2 IgG1 retarded tumor growth without detectable systemic toxicity. This anti-tumor effect was in part attributable to a potent ADCC response as well as direct cytotoxicity induced by the monoclonal antibody. Together, these results identify EMP2 as a novel therapeutic target for invasive breast cancer. PMID:24448822

Rationale and preclinical efficacy of a novel anti-EMP2 antibody for the treatment of invasive breast cancer.

PubMed

Fu, Maoyong; Maresh, Erin L; Helguera, Gustavo F; Kiyohara, Meagan; Qin, Yu; Ashki, Negin; Daniels-Wells, Tracy R; Aziz, Najib; Gordon, Lynn K; Braun, Jonathan; Elshimali, Yahya; Soslow, Robert A; Penichet, Manuel L; Goodglick, Lee; Wadehra, Madhuri

2014-04-01

Despite significant advances in biology and medicine, the incidence and mortality due to breast cancer worldwide is still unacceptably high. Thus, there is an urgent need to discover new molecular targets. In this article, we show evidence for a novel target in human breast cancer, the tetraspan protein epithelial membrane protein-2 (EMP2). Using tissue tumor arrays, protein expression of EMP2 was measured and found to be minimal in normal mammary tissue, but it was upregulated in 63% of invasive breast cancer tumors and in 73% of triple-negative tumors tested. To test the hypothesis that EMP2 may be a suitable target for therapy, we constructed a fully human immunoglobulin G1 (IgG1) antibody specific for a conserved domain of human and murine EMP2. Treatment of breast cancer cells with the anti-EMP2 IgG1 significantly inhibited EMP2-mediated signaling, blocked FAK/Src signaling, inhibited invasion, and promoted apoptosis in vitro. In both human xenograft and syngeneic metastatic tumor monotherapy models, anti-EMP2 IgG1 retarded tumor growth without detectable systemic toxicity. This antitumor effect was, in part, attributable to a potent antibody-dependent cell-mediated cytotoxicity response as well as direct cytotoxicity induced by the monoclonal antibody. Together, these results identify EMP2 as a novel therapeutic target for invasive breast cancer.

High resolution climate projection of storm surge at the Venetian coast

NASA Astrophysics Data System (ADS)

Mel, R.; Sterl, A.; Lionello, P.

2013-04-01

Climate change impact on storm surge regime is of great importance for the safety and maintenance of Venice. In this study a future storm surge scenario is evaluated using new high resolution sea level pressure and wind data recently produced by EC-Earth, an Earth System Model based on the operational seasonal forecast system of the European Centre for Medium-Range Weather Forecasts (ECMWF). The study considers an ensemble of six 5 yr long simulations of the rcp45 scenario at 0.25° resolution and compares the 2094-2098 to the 2004-2008 period. EC-Earth sea level pressure and surface wind fields are used as input for a shallow water hydrodynamic model (HYPSE) which computes sea level and barotropic currents in the Adriatic Sea. Results show that a high resolution climate model is needed for producing realistic values of storm surge statistics and confirm previous studies in that they show little sensitivity of storm surge levels to climate change. However, some climate change signals are detected, such as increased persistence of high pressure conditions, an increased frequency of windless hour, and a decreased number of moderate windstorms.

Tide-surge Interaction Intensified by the Taiwan Strait

NASA Astrophysics Data System (ADS)

Zhang, Wen-Zhou; Shi, Fengyan; Hong, Hua-Sheng; Shang, Shao-Ping; Kirby, James T.

2010-06-01

The Taiwan Strait is a long and wide shelf-channel where the hydrodynamics is extremely complex, being characterized by strong tides, and where storm surges frequently occur during the typhoon season. Obvious oscillations due to tide-surge interaction were observed by tide gauges along the northern Fujian coast, the west bank of the Taiwan Strait, during Typhoon Dan (1999). Numerical experiments indicate that nonlinear bottom friction (described by the quadratic formula) is a major factor to predict these oscillations while the nonlinear advective terms and the shallow water effect have little contribution. It is found that the tide-surge interaction in the northern portion of the Taiwan Strait is intensified by the strait. Simulations based on simplified topographies with and without the island of Taiwan show that, in the presence of the island, the channel effect strengthens tidal currents and tends to align the major axes of tidal ellipses along the channel direction. Storm-induced currents are also strengthened by the channel. The pattern of strong tidal currents and storm-induced currents along the channel direction enhances tide-surge interaction via the nonlinear bottom friction, resulting in the obvious oscillations along the northern Fujian coast.

Generation and detection of edge magnetoplasmons in a quantum Hall system using a photoconductive switch

NASA Astrophysics Data System (ADS)

Lin, Chaojing; Morita, Kyosuke; Muraki, Koji; Fujisawa, Toshimasa

2018-04-01

Edge magnetoplasmons (EMPs) are unidirectional charge density waves travelling in an edge channel of a two-dimensional electron gas in the quantum Hall regime. We present both generation and detection schemes with a photoconductive switch (PCS) for EMPs. Here, the conductance of the PCS is modulated by irradiation with a laser beam, whose amplitude can be modulated by an external signal. When the PCS is used as a generator, the electrical current from the PCS is injected into the edge channel to excite EMPs. When the PCS is used as a detector, the electronic potential induced by EMPs is applied to the PCS with a modulated laser beam so as to constitute a phase-sensitive measurement. For both experiments, we confirm that the time of flight for the EMPs increases with the magnetic field in agreement with the EMP characteristics. Combination of the two schemes would be useful in investigating and utilizing EMPs at higher frequencies.

Exploring the Early Chemical Evolution of the Milky Way with LAMOST and Subaru

NASA Astrophysics Data System (ADS)

Li, Haining; Aoki, Wako; Honda, Satoshi; Zhao, Gang; Suda, Takuma; Christlieb, Norbert

Extremely Metal-Poor (EMP) stars ([Fe/H] < -3.0) provide fundamental evidence on the nucleosynthesis and enrichment of the first stars and supernovae. LAMOST will observe 6 million Galactic stars through a 5-year spectroscopic survey, and thus provide an unprecedented chance to enlarge the EMP star sample. In 2014, a joint project on EMP stars was initiated with the LAMOST survey and Subaru follow-up observation. So far, more than 70 EMP stars have been found and confirmed, including identifications of a number of chemically interesting objects: three UMP (ultra metal-poor) stars with [Fe/H] ˜ -4.0, including the second UMP turnoff star with Li detection; a super Li-rich (A(Li) = +3) EMP giant, which is the most extreme example of Li enhancement in red giants known to date; a few EMP stars showing extreme enhancements in neutron-capture elements. Statistics of a large sample of EMP stars will constrain formation of the Milky Way halo.

The effect of channel deepening on tides and storm surge: A case study of Wilmington, NC

NASA Astrophysics Data System (ADS)

Familkhalili, R.; Talke, S. A.

2016-09-01

In this study we investigate the hypothesis that increasing channel depth in estuaries can amplify both tides and storm surge by developing an idealized numerical model representing the 1888, 1975, and 2015 bathymetric conditions of the Cape Fear River Estuary, NC. Archival tide gauge data recovered from the U.S. National Archives indicates that mean tidal range in Wilmington has doubled to 1.55 m since the 1880s, with a much smaller increase of 0.07 m observed near the ocean boundary. These tidal changes are reproduced by simulating channel depths of 7 m (1888 condition) and 15.5 m (modern condition). Similarly, model sensitivity studies using idealized, parametric tropical cyclones suggest that the storm surge in the worst-case, CAT-5 event may have increased from 3.8 ± 0.25 m to 5.6 ± 0.6 m since the nineteenth century. The amplification in both tides and storm surge is influenced by reduced hydraulic drag caused by greater mean depths.

Study to assess the effects of magnetohydrodynamic electromagnetic pulse on electric power systems, phase 1, volume 3

NASA Astrophysics Data System (ADS)

Legro, J. R.; Abi-Samra, N. C.; Tesche, F. M.

1985-05-01

In addition to the initial transients designated as fast transient high-altitude EMP (HEMP) and intermediate time EMP, electromagnetic signals are also perceived at times from seconds to hundreds of seconds after a high-altitude nuclear burst. This signal was defined by the term magnetohydrodynamic-electromagnetic pulse (MHD-EMP). The MHD-EMP phenomena was detected in actual weapon tests and predicted from theoretical models. A preliminary research effort to investigate the nature and coupling of the MHD-EMP environments to electric power systems documented the construction of approximate system response network models, and the development of a unified methodology to assess equipment and systematic vulnerability are defined. The MHD-EMP environment is compared to a qualitatively similar natural event, the electromagnetic environment produced by geomagnetic storms.

A High Density Storm Surge Monitoring Network: Evaluating the Ability of Wetland Vegetation to Reduce Storm Surge

NASA Astrophysics Data System (ADS)

Lawler, S.; Denton, M.; Ferreira, C.

2013-12-01

Recent tropical storm activity in the Chesapeake Bay and a potential increase in the predicted frequency and magnitude of weather systems have drawn increased attention to the need for improved tools for monitoring, modeling and predicting the magnitude of storm surge, coastal flooding and the respective damage to infrastructure and wetland ecosystems. Among other forms of flood protection, it is believed that coastal wetlands and vegetation can act as a natural barrier that slows hurricane flooding, helping to reduce the impact of storm surge. However, quantifying the relationship between the physical process of storm surge and its attenuation by wetland vegetation is an active area of research and the deployment of in-situ measuring devices is crucial to data collection efforts in this field. The United States Geological Survey (USGS) mobile storm-surge network has already successfully provided a framework for evaluating hurricane induced storm surge water levels on a regional scale through the use of in-situ devices installed in areas affected by storm surge during extreme events. Based on the success of the USGS efforts, in this study we adapted the monitoring network to cover relatively small areas of wetlands and coastal vegetation with an increased density of sensors. Groups of 6 to 10 water level sensors were installed in sites strategically selected in three locations on the Virginia coast of the lower Chesapeake Bay area to monitor different types of vegetation and the resulting hydrodynamic patterns (open coast and inland waters). Each group of sensors recorded time series data of water levels for both astronomical tide circulation and meteorological induced surge. Field campaigns were carried out to survey characteristics of vegetation contributing to flow resistance (i.e. height, diameter and stem density) and mapped using high precision GPS. A geodatabase containing data from field campaigns will support the development and calibration of computational models to simulate storm surge flow over wetlands specifically designed to represent Virginia's aquatic vegetation and to improve our fundamental knowledge of tide and storm surge hydrodynamics in estuarine wetlands. This poster will present the results of the field measurements for events during the 2013 Hurricane Season, tidal flows within the study areas, and surge attenuation rates according to vegetation characteristics.

A numerical study of vegetation impact on reducing storm surge by wetlands in a semi-enclosed estuary

USGS Publications Warehouse

Kelin, Hu; Qin, Chen; Wang, Hongqing

2014-01-01

Coastal wetlands play a unique role in extreme hurricane events. The impact of wetlands on storm surge depends on multiple factors including vegetation, landscape, and storm characteristics. The Delft3D model, in which vegetation effects on flow and turbulence are explicitly incorporated, was applied to the semi-enclosed Breton Sound (BS) estuary in coastal Louisiana to investigate the wetland impact. Guided by extensive field observations, a series of numerical experiments were conducted based on variations of actual vegetation properties and storm parameters from Hurricane Isaac in 2012. Both the vegetation-induced maximum surge reduction (MSR) and maximum surge reduction rate (MSRR) increased with stem height and stem density, and were more sensitive to stem height. The MSR and MSRR decreased significantly with increasing wind intensity. The MSRR was the highest with a fast-moving weak storm. It was also found that the MSRR varied proportionally to the expression involving the maximum bulk velocity and surge over the area of interest, and was more dependent on the maximum bulk surge. Both MSR and MSRR appeared to increase when the area of interest decreased from the whole BS estuary to the upper estuary. Within the range of the numerical experiments, the maximum simulated MSR and MSRR over the upper estuary were 0.7 m and 37%, respectively.

Dynamical Downscaling of Typhoon Vera (1959) and related Storm Surge based on JRA-55 Reanalysis

NASA Astrophysics Data System (ADS)

Ninomiya, J.; Takemi, T.; Mori, N.; Shibutani, Y.; Kim, S.

2015-12-01

Typhoon Vera in 1959 is historical extreme typhoon that caused severest typhoon damage mainly due to the storm surge up to 389 cm in Japan. Vera developed 895 hPa on offshore and landed with 929.2 hPa. There are many studies of the dynamical downscaling of Vera but it is difficult to simulate accurately because of the lack of the accuracy of global reanalysis data. This study carried out dynamical downscaling experiment of Vera using WRF downscaling forced by JRA-55 that are latest atmospheric model and reanalysis data. In this study, the reproducibility of five global reanalysis data for Typhoon Vera were compered. Comparison shows that reanalysis data doesn't have strong typhoon information except for JRA-55, so that downscaling with conventional reanalysis data goes wrong. The dynamical downscaling method for storm surge is studied very much (e.g. choice of physical model, nudging, 4D-VAR, bogus and so on). In this study, domain size and resolution of the coarse domain were considered. The coarse domain size influences the typhoon route and central pressure, and larger domain restrains the typhoon strength. The results of simulations with different domain size show that the threshold of developing restrain is whether the coarse domain fully includes the area of wind speed more than 15 m/s around the typhoon. The results of simulations with different resolution show that the resolution doesn't affect the typhoon route, and higher resolution gives stronger typhoon simulation.

EMP-002a Phase Shift through the Ionosphere

DOE Office of Scientific and Technical Information (OSTI.GOV)

Soltz, R.; Simons, D.; Fenimore, E.

2015-10-20

In this note we review the derivation and use of the Ionospheric Transfer Function (ITF) in the DIO- RAMA model to calculate the propagation of a broad band ElectroMagnetic Pulse (EMP) through the Ionosphere in the limit of geometric optics. This note is intended to resolve a misunderstanding between the NDS VVA and EMP modeling teams regarding the appropriate use of the phase and group velocities in DIORAMA. The di erent approaches are documented in EMP-002 note, \\Phase vs. Group" [1], generated by the LLNL DIORAMA VVA team, and the subsequent response from the DIORAMA EMP modeling team' [2].

Investigations on the effect of forage source, grinding, and urea supplementation on ruminal fermentation and microbial protein flow in a semi-continuous rumen simulation system.

PubMed

Hildebrand, Bastian; Boguhn, Jeannette; Rodehutscord, Markus

2011-10-01

The objective of the present study was to compare the effect of maize silage and grass silage on microbial fermentation and protein flow in a semi-continuous rumen simulation system (Rusitec) when milling screen size (MSS) during grinding was varied. Oven-dried silages were milled through screens of 1, 4 or 9 mm pore size and incubated for 48 h in a Rusitec system. Furthermore, the effect of N supplementation to maize silage (MSS: 4 mm) was investigated and single dose vs. continuous infusion of urea-N were compared. Degradation of organic matter (OM), crude protein (CP), fibre fractions and non-structural carbohydrates (NSC) as well as short-chain fatty acid production differed significantly between forage sources. Urea-N supplementation improved the degradation of NSC, but not that of fibre fractions in maize silage. The way of urea supply had only marginal effects on fermentation characteristics. An increase in MSS, and consequently in mean feed particle size, led to an improvement in the degradation of OM, CP and NSC, but efficiency of microbial net protein synthesis (EMPS; mg microbial N flow/g degraded OM) and the microbial amino acid profile were less affected. EMPS was higher in grass silage than in maize silage and was improved by urea-N supplementation in maize silage. This study indicates that fermentation of NSC as well as EMPS during incubation of maize silage was limited by availability of NH3-N. Furthermore, an increase in MSS above 1 mm seems to improve fermentation of silages in the Rusitec system.

Equivalent Air Spring Suspension Model for Quarter-Passive Model of Passenger Vehicles

PubMed Central

Abid, Haider J.; Chen, Jie; Nassar, Ameen A.

2015-01-01

This paper investigates the GENSIS air spring suspension system equivalence to a passive suspension system. The SIMULINK simulation together with the OptiY optimization is used to obtain the air spring suspension model equivalent to passive suspension system, where the car body response difference from both systems with the same road profile inputs is used as the objective function for optimization (OptiY program). The parameters of air spring system such as initial pressure, volume of bag, length of surge pipe, diameter of surge pipe, and volume of reservoir are obtained from optimization. The simulation results show that the air spring suspension equivalent system can produce responses very close to the passive suspension system. PMID:27351020

Comprehensive assessment of exposures to elongate mineral particles in the taconite mining industry.

PubMed

Hwang, Jooyeon; Ramachandran, Gurumurthy; Raynor, Peter C; Alexander, Bruce H; Mandel, Jeffrey H

2013-10-01

Since the 1970s, concerns have been raised about elevated rates of mesothelioma in the vicinity of the taconite mines in the Mesabi Iron Range. However, insufficient quantitative exposure data have hampered investigations of the relationship between cumulative exposures to elongate mineral particles (EMP) in taconite dust and adverse health effects. Specifically, no research on exposure to taconite dust, which includes EMP, has been conducted since 1990. This article describes a comprehensive assessment of present-day exposures to total and amphibole EMP in the taconite mining industry. Similar exposure groups (SEGs) were established to assess present-day exposure levels and buttress the sparse historical data. Personal samples were collected to assess the present-day levels of worker exposures to EMP at six mines in the Mesabi Iron Range. The samples were analyzed using National Institute for Occupational Safety and Health (NIOSH) methods 7400 and 7402. For many SEGs in several mines, the exposure levels of total EMP were higher than the NIOSH Recommended Exposure Limit (REL). However, the total EMP classification includes not only the asbestiform EMP and their non-asbestiform mineral analogs but also other minerals because the NIOSH 7400 cannot differentiate between these. The concentrations of amphibole EMP were well controlled across all mines and were much lower than the concentrations of total EMP, indicating that amphibole EMP are not major components of taconite EMP. The levels are also well below the NIOSH REL of 0.1 EMP cc(-1). Two different approaches were used to evaluate the variability of exposure between SEGs, between workers, and within workers. The related constructs of contrast and homogeneity were calculated to characterize the SEGs. Contrast, which is a ratio of between-SEG variability to the sum of between-SEG and between-worker variability, provides an overall measure of whether there are distinctions between the SEGs. Homogeneity, which is the ratio of the within-worker variance component to the sum of the between-worker and within-worker variance components, provides an overall measure of how similar exposures are for workers within an SEG. Using these constructs, it was determined that the SEGs are formed well enough when grouped by mine for both total and amphibole EMP to be used for epidemiological analysis.

Water Hammer Simulations of MMH Propellant - New Capability Demonstration of the Generalized Fluid Flow Simulation Program

NASA Technical Reports Server (NTRS)

Burkhardt, Z.; Ramachandran, N.; Majumdar, A.

2017-01-01

Fluid Transient analysis is important for the design of spacecraft propulsion system to ensure structural stability of the system in the event of sudden closing or opening of the valve. Generalized Fluid System Simulation Program (GFSSP), a general purpose flow network code developed at NASA/MSFC is capable of simulating pressure surge due to sudden opening or closing of valve when thermodynamic properties of real fluid are available for the entire range of simulation. Specifically GFSSP needs an accurate representation of pressure-density relationship in order to predict pressure surge during a fluid transient. Unfortunately, the available thermodynamic property programs such as REFPROP, GASP or GASPAK does not provide the thermodynamic properties of Monomethylhydrazine (MMH). This paper will illustrate the process used for building a customized table of properties of state variables from available properties and speed of sound that is required by GFSSP for simulation. Good agreement was found between the simulations and measured data. This method can be adopted for modeling flow networks and systems with other fluids whose properties are not known in detail in order to obtain general technical insight. Rigorous code validation of this approach will be done and reported at a future date.

B-Cell Responses to Pregnancy-Restricted and -Unrestricted Plasmodium falciparum Erythrocyte Membrane Protein 1 Antigens in Ghanaian Women Naturally Exposed to Malaria Parasites

PubMed Central

Ampomah, Paulina; Stevenson, Liz; Ofori, Michael F.; Barfod, Lea

2014-01-01

Protective immunity to Plasmodium falciparum malaria acquired after natural exposure is largely antibody mediated. IgG-specific P. falciparum EMP1 (PfEMP1) proteins on the infected erythrocyte surface are particularly important. The transient antibody responses and the slowly acquired protective immunity probably reflect the clonal antigenic variation and allelic polymorphism of PfEMP1. However, it is likely that other immune-evasive mechanisms are also involved, such as interference with formation and maintenance of immunological memory. We measured PfEMP1-specific antibody levels by enzyme-linked immunosorbent assay (ELISA) and memory B-cell frequencies by enzyme-linked immunosorbent spot (ELISPOT) assay in a cohort of P. falciparum-exposed nonpregnant Ghanaian women. The antigens used were a VAR2CSA-type PfEMP1 (IT4VAR04) with expression restricted to parasites infecting the placenta, as well as two commonly recognized PfEMP1 proteins (HB3VAR06 and IT4VAR60) implicated in rosetting and not pregnancy restricted. This enabled, for the first time, a direct comparison in the same individuals of immune responses specific for a clinically important parasite antigen expressed only during well-defined periods (pregnancy) to responses specific for comparable antigens expressed independent of pregnancy. Our data indicate that PfEMP1-specific B-cell memory is adequately acquired even when antigen exposure is infrequent (e.g., VAR2CSA-type PfEMP1). Furthermore, immunological memory specific for VAR2CSA-type PfEMP1 can be maintained for many years without antigen reexposure and after circulating antigen-specific IgG has disappeared. The study provides evidence that natural exposure to P. falciparum leads to formation of durable B-cell immunity to clinically important PfEMP1 antigens. This has encouraging implications for current efforts to develop PfEMP1-based vaccines. PMID:24566620

Flow cytometric detection of endothelial microparticles (EMP): effects of centrifugation and storage alter with the phenotype studied.

PubMed

van Ierssel, Sabrina H; Van Craenenbroeck, Emeline M; Conraads, Viviane M; Van Tendeloo, Viggo F; Vrints, Christiaan J; Jorens, Philippe G; Hoymans, Vicky Y

2010-04-01

Endothelial microparticles (EMP) are released into the circulation in case of endothelial disturbance, and are therefore increasingly investigated as a biomarker reflecting disease activity. Numerous pre-analytic methods have been proposed for their flow cytometric enumeration, but standardization is still lacking. In this study we evaluated the influence of centrifugation and storage conditions on EMP quantification. Platelet-poor plasma (PPP) from 10 healthy volunteers was prepared by centrifugation at 1,550 g for 20 minutes twice. A first aliquot of PPP was analyzed immediately, a second after storage at 4 degrees C for 7 hours. A third and fourth aliquot were snap-frozen and stored at -80 degrees C for 7 and 28 days. A final aliquot was further centrifuged at 10,000g for 10 minutes and analyzed immediately. EMP were defined as CD31+CD42b-, CD62E+, CD144+ or CD144+CD105+ particles, smaller than 1.0 microm. High speed centrifugation led to a significant loss of CD31+CD42b- EMP (p=0.004). A good correlation between PPP and high speed centrifuged PPP was only found for CD144+ EMP (Kendall tau b=0.611, p=0.025). Storage at 4 degrees C did not affect EMP quantification. However, freezing at -80 degrees C increased CD31+CD42b- and CD62E+ EMP counts, and lowered CD144+ EMP (p<0.05). Nevertheless, the agreement among the different storage conditions was relatively good (Kendall coefficient of concordance >0.487; p<0.05). The flow cytometric detection of EMP varies with the centrifugation protocol and the storage method used, and these changes also depend on the phenotype studied. The results of this study caution against comparing study results gathered with different EMP laboratory protocols. (c) 2010 Elsevier Ltd. All rights reserved.

Endothelial Microparticles (EMP) for the Assessment of Endothelial Function: An In Vitro and In Vivo Study on Possible Interference of Plasma Lipids

PubMed Central

van Ierssel, Sabrina H.; Hoymans, Vicky Y.; Van Craenenbroeck, Emeline M.; Van Tendeloo, Viggo F.; Vrints, Christiaan J.; Jorens, Philippe G.; Conraads, Viviane M.

2012-01-01

Background Circulating endothelial microparticles (EMP) reflect the condition of the endothelium and are of increasing interest in cardiovascular and inflammatory diseases. Recently, increased numbers of EMP following oral fat intake, possibly due to acute endothelial injury, have been reported. On the other hand, the direct interference of lipids with the detection of EMP has been suggested. This study aimed to investigate the effect of lipid-rich solutions, commonly administered in clinical practice, on the detection, both in vitro and in vivo, of EMP. Methods For the in vitro assessment, several lipid-rich solutions were added to whole blood of healthy subjects (n = 8) and patients with coronary heart disease (n = 5). EMP (CD31+/CD42b−) were detected in platelet poor plasma by flow cytometry. For the in vivo study, healthy volunteers were evaluated on 3 different study-days: baseline evaluation, following lipid infusion and after a NaCl infusion. EMP quantification, lipid measurements and peripheral arterial tonometry were performed on each day. Results Both in vitro addition and in vivo administration of lipids significantly decreased EMP (from 198.6 to 53.0 and from 272.6 to 90.6/µl PPP, respectively, p = 0.001 and p = 0.012). The EMP number correlated inversely with the concentration of triglycerides, both in vitro and in vivo (r = −0.707 and −0.589, p<0.001 and p = 0.021, respectively). The validity of EMP as a marker of endothelial function is supported by their inverse relationship with the reactive hyperemia index (r = −0.758, p = 0.011). This inverse relation was confounded by the intravenous administration of lipids. Conclusion The confounding effect of high circulating levels of lipids, commonly found in patients that receive intravenous lipid-based solutions, should be taken into account when flow cytometry is used to quantify EMP. PMID:22359595

Endothelial microparticles (EMP) for the assessment of endothelial function: an in vitro and in vivo study on possible interference of plasma lipids.

PubMed

van Ierssel, Sabrina H; Hoymans, Vicky Y; Van Craenenbroeck, Emeline M; Van Tendeloo, Viggo F; Vrints, Christiaan J; Jorens, Philippe G; Conraads, Viviane M

2012-01-01

Circulating endothelial microparticles (EMP) reflect the condition of the endothelium and are of increasing interest in cardiovascular and inflammatory diseases. Recently, increased numbers of EMP following oral fat intake, possibly due to acute endothelial injury, have been reported. On the other hand, the direct interference of lipids with the detection of EMP has been suggested. This study aimed to investigate the effect of lipid-rich solutions, commonly administered in clinical practice, on the detection, both in vitro and in vivo, of EMP. For the in vitro assessment, several lipid-rich solutions were added to whole blood of healthy subjects (n = 8) and patients with coronary heart disease (n = 5). EMP (CD31+/CD42b-) were detected in platelet poor plasma by flow cytometry. For the in vivo study, healthy volunteers were evaluated on 3 different study-days: baseline evaluation, following lipid infusion and after a NaCl infusion. EMP quantification, lipid measurements and peripheral arterial tonometry were performed on each day. Both in vitro addition and in vivo administration of lipids significantly decreased EMP (from 198.6 to 53.0 and from 272.6 to 90.6/µl PPP, respectively, p = 0.001 and p = 0.012). The EMP number correlated inversely with the concentration of triglycerides, both in vitro and in vivo (r = -0.707 and -0.589, p<0.001 and p = 0.021, respectively). The validity of EMP as a marker of endothelial function is supported by their inverse relationship with the reactive hyperemia index (r = -0.758, p = 0.011). This inverse relation was confounded by the intravenous administration of lipids. The confounding effect of high circulating levels of lipids, commonly found in patients that receive intravenous lipid-based solutions, should be taken into account when flow cytometry is used to quantify EMP.

A numerical study on the effects of wave-current-surge interactions on the height and propagation of sea surface waves in Charleston Harbor during Hurricane Hugo 1989

NASA Astrophysics Data System (ADS)

Liu, Huiqing; Xie, Lian

2009-06-01

The effects of wave-current interactions on ocean surface waves induced by Hurricane Hugo in and around the Charleston Harbor and its adjacent coastal waters are examined by using a three-dimensional (3D) wave-current coupled modeling system. The 3D storm surge modeling component of the coupled system is based on the Princeton Ocean Model (POM), the wave modeling component is based on the third generation wave model, Simulating WAves Nearshore (SWAN), and the inundation model is adopted from [Xie, L., Pietrafesa, L. J., Peng, M., 2004. Incorporation of a mass-conserving inundation scheme into a three-dimensional storm surge model. J. Coastal Res., 20, 1209-1223]. The results indicate that the change of water level associated with the storm surge is the primary cause for wave height changes due to wave-surge interaction. Meanwhile, waves propagating on top of surge cause a feedback effect on the surge height by modulating the surface wind stress and bottom stress. This effect is significant in shallow coastal waters, but relatively small in offshore deep waters. The influence of wave-current interaction on wave propagation is relatively insignificant, since waves generally propagate in the direction of the surface currents driven by winds. Wave-current interactions also affect the surface waves as a result of inundation and drying induced by the storm. Waves break as waters retreat in regions of drying, whereas waves are generated in flooded regions where no waves would have occurred without the flood water.

Multi-hazard risk analysis related to hurricanes

NASA Astrophysics Data System (ADS)

Lin, Ning

Hurricanes present major hazards to the United States. Associated with extreme winds, heavy rainfall, and storm surge, landfalling hurricanes often cause enormous structural damage to coastal regions. Hurricane damage risk assessment provides the basis for loss mitigation and related policy-making. Current hurricane risk models, however, often oversimplify the complex processes of hurricane damage. This dissertation aims to improve existing hurricane risk assessment methodology by coherently modeling the spatial-temporal processes of storm landfall, hazards, and damage. Numerical modeling technologies are used to investigate the multiplicity of hazards associated with landfalling hurricanes. The application and effectiveness of current weather forecasting technologies to predict hurricane hazards is investigated. In particular, the Weather Research and Forecasting model (WRF), with Geophysical Fluid Dynamics Laboratory (GFDL)'s hurricane initialization scheme, is applied to the simulation of the wind and rainfall environment during hurricane landfall. The WRF model is further coupled with the Advanced Circulation (AD-CIRC) model to simulate storm surge in coastal regions. A case study examines the multiple hazards associated with Hurricane Isabel (2003). Also, a risk assessment methodology is developed to estimate the probability distribution of hurricane storm surge heights along the coast, particularly for data-scarce regions, such as New York City. This methodology makes use of relatively simple models, specifically a statistical/deterministic hurricane model and the Sea, Lake and Overland Surges from Hurricanes (SLOSH) model, to simulate large numbers of synthetic surge events, and conducts statistical analysis. The estimation of hurricane landfall probability and hazards are combined with structural vulnerability models to estimate hurricane damage risk. Wind-induced damage mechanisms are extensively studied. An innovative windborne debris risk model is developed based on the theory of Poisson random measure, substantiated by a large amount of empirical data. An advanced vulnerability assessment methodology is then developed, by integrating this debris risk model and a component-based pressure damage model, to predict storm-specific or annual damage to coastal residential neighborhoods. The uniqueness of this vulnerability model lies in its detailed description of the interaction between wind pressure and windborne debris effects over periods of strong winds, which is a major mechanism leading to structural failures during hurricanes.

Improving the Resilience of Major Ports and Critical Supply Chains to Extreme Coastal Flooding: a Combined Artificial Neural Network and Hydrodynamic Simulation Approach to Predicting Tidal Surge Inundation of Port Infrastructure and Impact on Operations.

NASA Astrophysics Data System (ADS)

French, J.

2015-12-01

Ports are vital to the global economy, but assessments of global exposure to flood risk have generally focused on major concentrations of population or asset values. Few studies have examined the impact of extreme inundation events on port operation and critical supply chains. Extreme water levels and recurrence intervals have conventionally been estimated via analysis of historic water level maxima, and these vary widely depending on the statistical assumptions made. This information is supplemented by near-term forecasts from operational surge-tide models, which give continuous water levels but at considerable computational cost. As part of a NERC Infrastructure and Risk project, we have investigated the impact of North Sea tidal surges on the Port of Immingham, eastern, UK. This handles the largest volume of bulk cargo in the UK and flows of coal and biomass that are critically important for national energy security. The port was partly flooded during a major tidal surge in 2013. This event highlighted the need for improved local forecasts of surge timing in relation to high water, with a better indication of flood depth and duration. We address this problem using a combination of data-driven and numerical hydrodynamic models. An Artificial Neural Network (ANN) is first used to predict the surge component of water level from meteorological data. The input vector comprises time-series of local wind (easterly and northerly wind stress) and pressure, as well as regional pressure and pressure gradients from stations between the Shetland Islands and the Humber estuary. The ANN achieves rms errors of around 0.1 m and can generate short-range (~ 3 to 12 hour) forecasts given real-time input data feeds. It can also synthesize water level events for a wider range of tidal and meteorological forcing combinations than contained in the observational records. These are used to force Telemac2D numerical floodplain simulations using a LiDAR digital elevation model of the port. Functional relationships between peak water level and surge 'shape' allow estimation of flood depths and durations for any location. Supplementing existing surge warning systems, our approach predicts the location and duration of flooding in detail, and allows port managers to take steps to minimize its impact on the most critical aspects of port operation.

Electromagnetic-guided placement of nasoduodenal feeding tubes versus endoscopic placement: a randomized, multicenter trial.

PubMed

Kappelle, Wouter F W; Walter, Daisy; Stadhouders, Paul H; Jebbink, Hendrik J A; Vleggaar, Frank P; van der Schaar, Peter J; Kappelle, Jan Willem; van der Tweel, Ingeborg; Van den Broek, Medard F M; Wessels, Frank J; Siersema, Peter D; Monkelbaan, Jan F

2018-01-01

Electromagnetic-guided placement (EMP) of a nasoduodenal feeding tube by trained nurses is an attractive alternative to EGD-guided placement (EGDP). We aimed to compare EMP and EGDP in outpatients, ward patients, and critically ill patients with normal upper GI anatomy. In 3 centers with no prior experience in EMP, patients were randomized to placement of a single-lumen nasoduodenal feeding tube either with EGDP or EMP. The primary endpoint was post-pyloric position of the tube on abdominal radiography. Patients were followed for 10 days to assess patency and adverse events. The analyses were performed according to the intention-to-treat principle. In total, 160 patients were randomized to EGDP (N = 76) or EMP (N = 84). Three patients withdrew informed consent, and no abdominal radiography was performed in 2 patients. Thus, 155 patients (59 intensive care unit, 38%) were included in the analyses. Rates of post-pyloric tube position between EGDP and EMP were comparable (79% vs 82%, odds ratio 1.16; 90% confidence interval, 0.58-2.38; P = .72). Adverse events were observed in 4 patients after EMP (hypoxia, GI blood loss, atrial fibrillation, abdominal pain) and in 4 after EGDP (epistaxis N = 2, GI blood loss, hypoxia). Costs of tube placements were lower for EMP compared with EGDP: $519.09 versus $622.49, respectively (P = .04). Success rates and safety of EMP and EGDP in patients with normal upper GI anatomy were comparable. Lower costs and potential logistic advantages may drive centers to adopt EMP as their new standard of care. (Clinical trial registration number: NTR4286.). Copyright © 2018 American Society for Gastrointestinal Endoscopy. Published by Elsevier Inc. All rights reserved.

The association between endothelial microparticles and inflammation in patients with systemic sclerosis and Raynaud's phenomenon as detected by functional imaging.

PubMed

Jung, Christian; Drummer, Karl; Oelzner, Peter; Figulla, Hans R; Boettcher, Joachim; Franz, Marcus; Betge, Stefan; Foerster, Martin; Wolf, Gunter; Pfeil, Alexander

2015-01-01

Systemic sclerosis (SSc) is a systemic, autoimmune connective tissue disease characterized by vasculopathy and microvascular changes. Fluorescence Optical Imaging (FOI) is a technique used to assess inflammation in patients with arthritis; in this study FOI is used to quantify inflammation in the hand. Endothelial Microparticle (EMP) can reflect damage or activation of the endothelium but also actively modulate processes of inflammation, coagulation and vascular function. The aim of the present study was to quantify EMP and FOI, to determine an association between these microparticles and inflammation and to endothelial function. EMP were quantified in plasma samples of 25 patients (24 female, 1 male, age: 41 ± 9 years) with SSc using flow cytometry. EMP was defined as CD31+/CD42- MP, and CD62+ MP. Perivascular inflammation was assessed using fluorescence optical imaging (FOI) of the hand. Macrovascular endothelial function was non-invasively estimated using the Endopat system. Plasma levels of CD31+/CD42- EMP and CD62+ EMP were lower in patients with SSc compared to controls (both p <  0.05). An impaired endothelial function with an increased hyperemia index was observed. A strong association could be demonstrated between CD62+ EMP and perivascular soft tissue inflammation as assessed by the FOI global score (Spearman, p = 0.002, r = 0.61). EMP indicate molecular vascular damage in SSc; in this study a strong association between EMP and perivascular inflammation as quantified by FOI is demonstrated. Consequently EMP, using FOI, may be a potential marker benefitting the diagnosis and therapy monitoring of patients with SSc with associated Raynaud's phenomenon.

Low Frequency Electromagnetic Pulse and Explosions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sweeney, J J

2011-02-01

This paper reviews and summarizes prior work related to low frequency (< 100 Hz) EMP (ElectroMagnetic Pulse) observed from explosions. It focuses on how EMP signals might, or might not, be useful in monitoring underground nuclear tests, based on the limits of detection, and physical understanding of these signals. In summary: (1) Both chemical and nuclear explosions produce an EMP. (2) The amplitude of the EMP from underground explosions is at least two orders of magnitude lower than from above ground explosions and higher frequency components of the signal are rapidly attenuated due to ground conductivity. (3) In general, inmore » the near field, that is distances (r) of less than 10s of kilometers from the source, the amplitude of the EMP decays approximately as 1/r{sup 3}, which practically limits EMP applications to very close (<{approx}1km) distances. (4) One computational model suggests that the EMP from a decoupled nuclear explosion may be enhanced over the fully coupled case. This has not been validated with laboratory or field data. (5) The magnitude of the EMP from an underground nuclear explosion is about two orders of magnitude larger than that from a chemical explosion, and has a larger component of higher frequencies. In principle these differences might be used to discriminate a nuclear from a chemical explosion using sensors at very close (<{approx}1 km) distances. (6) Arming and firing systems (e.g. detonators, exploding bridge wires) can also produce an EMP from any type of explosion. (7) To develop the understanding needed to apply low frequency EMP to nuclear explosion monitoring, it is recommended to carry out a series of controlled underground chemical explosions with a variety of sizes, emplacements (e.g. fully coupled and decoupled), and arming and firing systems.« less

Physical properties of elongated magnetic particles: magnetization and friction coefficient anisotropies.

PubMed

Vereda, Fernando; de Vicente, Juan; Hidalgo-Alvarez, Roque

2009-06-02

Anisotropy counts: A brief review of the main physical properties of elongated magnetic particles (EMPs) is presented. The most important characteristic of an EMP is the additional contribution of shape anisotropy to the total anisotropy energy of the particle, when compared to spherical magnetic particles. The electron micrograph shows Ni-ferrite microrods fabricated by the authors.We present an overview of the main physical properties of elongated magnetic particles (EMPs), including some of their more relevant properties in suspension. When compared to a spherical magnetic particle, the most important characteristic of an EMP is an additional contribution of shape anisotropy to the total anisotropy energy of the particle. Increasing aspect ratios also lead to an increase in both the critical single-domain size of a magnetic particle and its resistance to thermally activated spontaneous reversal of the magnetization. For single-domain EMPs, magnetization reversal occurs primarily by one of two modes, coherent rotation or curling, the latter being facilitated by larger aspect ratios. When EMPs are used to prepare colloidal suspensions, other physical properties come into play, such as their anisotropic friction coefficient and the consequent enhanced torque they experience in a shear flow, their tendency to align in the direction of an external field, to form less dense sediments and to entangle into more intricate aggregates. From a more practical point of view, EMPs are discussed in connection with two interesting types of magnetic colloids: magnetorheological fluids and suspensions for magnetic hyperthermia. Advances reported in the literature regarding the use of EMPs in these two systems are included. In the final section, we present a summary of the most relevant methods documented in the literature for the fabrication of EMPs, together with a list of the most common ferromagnetic materials that have been synthesized in the form of EMPs.

[Apoptosis of human lung carcinoma cell line GLC-82 induced by high power electromagnetic pulse].

PubMed

Cao, Xiao-zhe; Zhao, Mei-lan; Wang, De-wen; Dong, Bo

2002-09-01

Electromagnetic pulse (EMP) could be used for sterilization of food and the efficiency is higher than 2450 MHz continuous microwave done. This study was designed to evaluate the effect of electromagnetic pulse (EMP) on apoptosis of human lung carcinoma cell line GLC-82, so that to explore and develop therapeutic means for cancer. The injury changes in GLC-82 cells after irradiated with EMP (electric field intensity was 60 kV/m, 5 pulses/2 min) were analyzed by cytometry, MTT chronometry, and flow cytometry. The immunohistochemical SP staining was used to determine the expressions of bcl-2 protein and p53 protein. The stained positive cells were analyzed by CMIAS-II image analysis system at a magnification 400. All data were analyzed by SPSS8.0 software. EMP could obviously inhibited proliferation and activity of lung carcinoma cell line GLC-82. The absorbance value (A570) of MTT decreased immediately, at 0 h, 1 h, and 6 h after the GLC-82 cells irradiated by EMP as compared with control group. The highest apoptosis rate was found to reach 13.38% by flow cytometry at 6 h after EMP irradiation. Down-regulation of bcl-2 expression and up-regulation of p53 expression were induced by EMP. EMP promotes apoptosis of GLC-82 cells. At same time, EMP can down-regulate bcl-2 expression and up-regulate p53 expression in GLC-82 cells. The bcl-2 and the p53 protein may involve the apoptotic process.

Elevated circulating endothelial cell-derived microparticle levels in patients with liver cirrhosis: a preliminary report

PubMed Central

Simon, Krzysztof Adam; Pazgan-Simon, Monika

2015-01-01

Aim of the study To determine plausible associations between liver cirrhosis and circulating endothelial cell-derived microparticles (EMPs), vascular endothelial growth factor (VEGF) levels and plasma nitric oxide (NO) metabolites. Material and methods Sixty patients with cirrhosis and 20 healthy control subjects were enrolled in the study. Circulating EMPs from platelet-poor plasma samples were examined by flow cytometry. These microparticles were categorized into endothelial cell-derived activated MPs (EMP-ac) (CD31+ CD42b– AN-V–) and endothelial cell-derived apoptotic MPs (EMP-ap) (CD31+ CD42b– AN-V+). Plasma VEGF levels were measured by enzyme-linked immunosorbent assay. Plasma NO metabolites (NOx–) levels were determined using a Greiss reaction method. Results Compared with the healthy control subjects, the patients with cirrhosis showed a significant increase in plasma levels of both phenotypes of EMPs. When the presence of ascites was considered, the plasma levels of EMP-ap were higher (p < 0.01), as well as NOx– (p < 0.05). EMP-ap positively correlated with VEGF level in all cirrhotic patients and this correlation was stronger in decompensated cirrhotic patients. In multivariate logistic regression analysis, the independent factors associated with the presence of ascites were high EMP-ap levels and elevated VEGF levels. Conclusions Elevated plasma levels of EMP-ap in addition to high levels of VEGF might be considered as valuable parameters for predicting the occurrence of ascites in cirrhotic patients. PMID:28856256

Mesothelioma and other lung disease in taconite miners; the uncertain role of non-asbestiform EMP.

PubMed

Mandel, Jeffrey H; Odo, Nnaemeka U

2018-04-10

The purpose of this paper was to assess the role of non-asbestiform amphibole EMPs in the etiology of mesotheliomas and other lung disease in taconite (iron ore) miners. Increased mesothelioma rates have been described in Minnesota taconite workers since the late 1990s. Currently, over 100 cases have been reported by the Minnesota Department of Health within the complete cohort of miners in Minnesota. Geologic sampling has indicated that only the eastern part of the iron range contains non-asbestiform amphibole elongate mineral particles (EMPs), in close proximity to the ore. This type of EMP has been less studied and also exists in talc and gold mining. A series of investigations into the state's taconite industry have been recently completed. Results from a cohort mortality study indicated an SMR of 2.77 (95% CI = 1.87-3.96) for mesothelioma. In a case-control study, the odds ratio for mesothelioma for high vs. low EMP exposure was 2.25 (5% CI = 1.13-4.5) but EMPs in this study were counted by phase contrast microscopy. Odds ratios were not elevated in mines located in the eastern part of the Mesabi iron range. The overall findings suggest that mesothelioma in taconite miners is related to EMP exposure. Because of the way EMPs were counted, results from these studies cannot allow a firm conclusion about the association between EMP exposure and the reported excess mesothelioma. Copyright © 2018 Elsevier Inc. All rights reserved.

Classification of extremely metal-poor stars: absent region in A(C)-[Fe/H] plane and the role of dust cooling

NASA Astrophysics Data System (ADS)

Chiaki, Gen; Tominaga, Nozomu; Nozawa, Takaya

2017-11-01

Extremely metal-poor (EMP) stars are the living fossils with records of chemical enrichment history at the early epoch of galaxy formation. By the recent large observation campaigns, statistical samples of EMP stars have been obtained. This motivates us to reconsider their classification and formation conditions. From the observed lower limits of carbon and iron abundances of Acr(C) ∼ 6 and [Fe/H]cr ∼ -5 for C-enhanced EMP (CE-EMP) and C-normal EMP (CN-EMP) stars, we confirm that gas cooling by dust thermal emission is indispensable for the fragmentation of their parent clouds to form such low mass, i.e. long-lived stars, and that the dominant grain species are carbon and silicate, respectively. We constrain the grain radius r_i^cool of a species i and condensation efficiency fij of a key element j as r_C^cool / f_C,C = 10 {μ m} and r_Sil^cool / f_Sil,Mg = 0.1 {μ m} to reproduce Acr(C) and [Fe/H]cr, which give a universal condition 10[C/H] - 2.30 + 10[Fe/H] > 10-5.07 for the formation of every EMP star. Instead of the conventional boundary [C/Fe] = 0.7 between CE-EMP and CN-EMP stars, this condition suggests a physically meaningful boundary [C/Fe]b = 2.30 above and below which carbon and silicate grains are dominant coolants, respectively.

Assessment of the Temporal Evolution of Storm Surge via Land to Water Isopleths in Coastal Louisiana

NASA Astrophysics Data System (ADS)

Siverd, C. G.; Hagen, S. C.; Bilskie, M. V.; Braud, D.; Gao, S.; Peele, H.; Twilley, R.

2017-12-01

The low-lying coastal Louisiana deltaic landscape features an intricate system of fragmented wetlands, natural ridges, man-made navigation canals and flood protection infrastructure. Since 1900 and prior to the landfall of Hurricane Katrina in 2005, Louisiana lost approximately 480,000 ha (1,850 sq mi) of coastal wetlands and an additional 20,000 ha (77 sq mi) due to Katrina. This resulted in a total wetland storm protection value loss of USD 28.3 billion and USD 1.1 billion, respectively (Costanza 2008). To investigate the response of hurricane storm surge (e.g. peak water levels, inundation time and extent) through time due to land loss, hydrodynamic models that represent historical eras of the Louisiana coastal landscape were developed. Land:Water (L:W) isopleths (Gagliano 1970, 1971, Twilley 2016) have been calculated along the coast from the Sabine River to the Pearl River. These isopleths were utilized to create a simplified coastal landscape (bathymetry, topography, bottom roughness) representing circa 2010. Similar methodologies are employed with the objective of developing storm surge models that represent the coastal landscape for past eras. The goal is to temporally examine the evolution of storm surge along coastal Louisiana. The isopleths determined to best represent the Louisiana coast as a result of the methodology devised to develop the simple storm surge model for c.2010 are applied in the development of surge models for historical eras c.1930 and c.1970. The ADvaced CIRCulation (ADCIRC) code (Luettich 2004) is used to perform storm surge simulations with a predetermined suite of hurricane wind and pressure forcings. Hydrologic Unit Code 12 (HUC12) sub-watersheds provide geographical bounds to quantify mean maximum water surface elevations (WSEs), volume of inundation, and area of inundation. HUC12 sub-watersheds also provide a means to compare/contrast these quantified surge parameters on a HUC12-by-HUC12 basis for the c.1930, c.1970 and c.2010 eras. Results will provide insight into how storm surge has evolved in coastal Louisiana from 1930 to 2010 and assist to inform policy makers of regions with temporally accelerating storm surge.

Effect of electromagnetic pulse exposure on brain micro vascular permeability in rats.

PubMed

Ding, Gui-Rong; Li, Kang-Chu; Wang, Xiao-Wu; Zhou, Yong-Chun; Qiu, Lian-Bo; Tan, Juan; Xu, Sheng-Long; Guo, Guo-Zhen

2009-06-01

To observe the effect of electromagnetic pulse (EMP) exposure on cerebral micro vascular permeability in rats. The whole-body of male Sprague-Dawley rats were exposed or sham exposed to 200 pulses or 400 pulses (1 Hz) of EMP at 200 kV/m. At 0.5, 1, 3, 6, and 12 h after EMP exposure, the permeability of cerebral micro vascular was detected by transmission electron microscopy and immunohistochemistry using lanthanum nitrate and endogenous albumin as vascular tracers, respectively. The lanthanum nitrate tracer was limited to the micro vascular lumen with no lanthanum nitrate or albumin tracer extravasation in control rat brain. After EMP exposure, the lanthanum nitrate ions reached the tight junction, basal lamina and pericapillary tissue. Similarly, the albumin immunopositive staining was identified in pericapillary tissue. The changes in brain micro vascular permeability were transient, the leakage of micro vascular vessels appeared at 1 h, and reached its peak at 3 h, and nearly recovered at 12 h, after EMP exposure. In addition, the leakage of micro vascular was more obvious after exposure of EMP at 400 pulses than after exposure of EMP at 200 pulses. Exposure to 200 and 400 pulses (1 Hz) of EMP at 200 kV/m can increase cerebral micro vascular permeability in rats, which is recoverable.

Neuroprotective Effects of Sevoflurane against Electromagnetic Pulse-Induced Brain Injury through Inhibition of Neuronal Oxidative Stress and Apoptosis

PubMed Central

Wang, Jin; Han, Li-Chun; Li, Li-Ya; Wu, Guang-Li; Hou, Yan-Ning; Guo, Guo-Zhen; Wang, Qiang; Sang, Han-Fei; Xu, Li-Xian

2014-01-01

Electromagnetic pulse (EMP) causes central nervous system damage and neurobehavioral disorders, and sevoflurane protects the brain from ischemic injury. We investigated the effects of sevoflurane on EMP-induced brain injury. Rats were exposed to EMP and immediately treated with sevoflurane. The protective effects of sevoflurane were assessed by Nissl staining, Fluoro-Jade C staining and electron microscopy. The neurobehavioral effects were assessed using the open-field test and the Morris water maze. Finally, primary cerebral cortical neurons were exposed to EMP and incubated with different concentration of sevoflurane. The cellular viability, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were assayed. TUNEL staining was performed, and the expression of apoptotic markers was determined. The cerebral cortexes of EMP-exposed rats presented neuronal abnormalities. Sevoflurane alleviated these effects, as well as the learning and memory deficits caused by EMP exposure. In vitro, cell viability was reduced and LDH release was increased after EMP exposure; treatment with sevoflurane ameliorated these effects. Additionally, sevoflurane increased SOD activity, decreased MDA levels and alleviated neuronal apoptosis by regulating the expression of cleaved caspase-3, Bax and Bcl-2. These findings demonstrate that Sevoflurane conferred neuroprotective effects against EMP radiation-induced brain damage by inhibiting neuronal oxidative stress and apoptosis. PMID:24614080

Neuroprotective effects of sevoflurane against electromagnetic pulse-induced brain injury through inhibition of neuronal oxidative stress and apoptosis.

PubMed

Deng, Bin; Xu, Hao; Zhang, Jin; Wang, Jin; Han, Li-Chun; Li, Li-Ya; Wu, Guang-Li; Hou, Yan-Ning; Guo, Guo-Zhen; Wang, Qiang; Sang, Han-Fei; Xu, Li-Xian

2014-01-01

Electromagnetic pulse (EMP) causes central nervous system damage and neurobehavioral disorders, and sevoflurane protects the brain from ischemic injury. We investigated the effects of sevoflurane on EMP-induced brain injury. Rats were exposed to EMP and immediately treated with sevoflurane. The protective effects of sevoflurane were assessed by Nissl staining, Fluoro-Jade C staining and electron microscopy. The neurobehavioral effects were assessed using the open-field test and the Morris water maze. Finally, primary cerebral cortical neurons were exposed to EMP and incubated with different concentration of sevoflurane. The cellular viability, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were assayed. TUNEL staining was performed, and the expression of apoptotic markers was determined. The cerebral cortexes of EMP-exposed rats presented neuronal abnormalities. Sevoflurane alleviated these effects, as well as the learning and memory deficits caused by EMP exposure. In vitro, cell viability was reduced and LDH release was increased after EMP exposure; treatment with sevoflurane ameliorated these effects. Additionally, sevoflurane increased SOD activity, decreased MDA levels and alleviated neuronal apoptosis by regulating the expression of cleaved caspase-3, Bax and Bcl-2. These findings demonstrate that Sevoflurane conferred neuroprotective effects against EMP radiation-induced brain damage by inhibiting neuronal oxidative stress and apoptosis.

The expression and function of epithelial membrane protein 1 in laryngeal carcinoma.

PubMed

Li, Hong; Zhang, Xiaowen; Jiang, Xuejun; Ji, Xu

2017-01-01

In this study, we compared the expression of epithelial membrane protein 1 (EMP1) on the steady-state mRNA level (by quantitative real-time PCR) and on the protein level (by western immunoblot and immunohistochemistry) in 51 pairs of laryngeal carcinoma tissues and matched cancer-free peritumor tissues, and we analyzed the correlation between EMP1 expression and different clinicopathological factors. Furthermore, we ectopically expressed EMP1 in human laryngeal carcinoma Hep-2 cells and examined the effects on cell viability, apoptosis, colonogenicity, and motility, by MTT assay, flow cytometry, colony formation assay and Transwell migration assay, respectively. EMP1 expression (on both the mRNA and protein levels) was significantly lower in the cancer tissues than in matched peritumor tissues (P<0.05). In laryngeal cancers, the level of EMP1 protein was correlated with histological grade (P<0.05), but not with age, gender, clinical stage, cancer subtype or lymph node metastasis (P>0.05). Functionally, ectopic expression of EMP1 in Hep-2 cells significantly reduced cell viability, colony formation, and migration, but enhanced apoptosis. Therefore, EMP1 is a tumor suppressor in laryngeal carcinoma. Boosting EMP1 expression in laryngeal carcinoma initiates multiple anticancer phenotypes and thus presents a promising therapeutic strategy for laryngeal cancer.

Novel interactions between erythroblast macrophage protein and cell migration.

PubMed

Javan, Gulnaz T; Can, Ismail; Yeboah, Fred; Lee, Youngil; Soni, Shivani

2016-09-01

Erythroblast macrophage protein is a novel protein known to mediate attachment of erythroid cells to macrophages to form erythroblastic islands in bone marrow during erythropoiesis. Emp-null macrophages are small with round morphologies, and lack cytoplasmic projections which imply immature structure. The role of Emp in macrophage development and function is not fully elucidated. Macrophages perform varied functions (e.g. homeostasis, erythropoiesis), and are implicated in numerous pathophysiological conditions such as cellular malignancy. The objective of the current study is to investigate the interaction of Emp with cytoskeletal- and cell migration-associated proteins involved in macrophage functions. A short hairpin RNA lentiviral system was use to down-regulate the expression of Emp in macrophage cells. A cell migration assay revealed that the relocation of macrophages was significantly inhibited when Emp expression was decreased. To further analyze changes in gene expression related to cell motility, PCR array was performed by down-regulating Emp expression. The results indicated that expression of mitogen-activated protein kinase 1 and thymoma viral proto-oncogene 1 were significantly higher when Emp was down-regulated. The results implicate Emp in abnormal cell motility, thus, warrants to assess its role in cancer where tumor cell motility is required for invasion and metastasis. Copyright © 2016 Elsevier Inc. All rights reserved.

Erythroblast macrophage protein (Emp): Past, present, and future.

PubMed

Javan, Gulnaz T; Salhotra, Amandeep; Finley, Sheree J; Soni, Shivani

2018-01-01

This review is a journey of the landmark erythroblast macrophage protein (Emp) discovered in 1994, and it walks chronologically through the progress that has been made in understanding the biological function of this protein. Historically, Emp was the first identified cell attachment molecule and is expressed in both erythroblasts and macrophages and mediates their attachments to form erythroblastic islands. The absence of Emp erythroblasts shows defects in differentiation and enucleation. Emp-deficient macrophages display immature morphology characterized by small sizes, round shapes, and the lack of cytoplasmic projections. Although the primary sequence of Emp has already been determined and its role in both erythroid and macrophage development is well established, there are major gaps in the understanding of its function at the molecular level. Recent studies had implicated its importance in actin cytoskeleton remodeling and cell migration, but the molecular mechanisms are still enigmatic. Previous studies have also demonstrated that downregulation of Emp affects the expression of mitogen-associated protein kinase 1 (MAPK1) and thymoma viral protooncogene (AKT-1) resulting in abnormal cell motility. In this review, we summarize the proposed function of Emp based on previous studies, present scenarios, and its plausible future in translational research. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Hydraulic modeling of unsteady debris-flow surges with solid-fluid interactions

USGS Publications Warehouse

Iverson, Richard M.

1997-01-01

Interactions of solid and fluid constituents produce the unique style of motion that typifies debris flows. To simulate this motion, a new hydraulic model represents debris flows as deforming masses of granular solids variably liquefied by viscous pore fluid. The momentum equation of the model describes how internal and boundary forces change as coarse-grained surge heads dominated by grain-contact friction grade into muddy debris-flow bodies more strongly influenced by fluid viscosity and pressure. Scaling analysis reveals that pore-pressure variations can cause flow resistance in surge heads to surpass that in debris-flow bodies by orders of magnitude. Numerical solutions of the coupled momentum and continuity equations provide good predictions of unsteady, nonuniform motion of experimental debris flows from initiation through deposition.

The GP tests of competence assessment: which part best predicts fitness to practise decisions?

PubMed

Jayaweera, Hirosha Keshani; Potts, Henry W W; Keshwani, Karim; Valerio, Chris; Baker, Magdalen; Mehdizadeh, Leila; Sturrock, Alison

2018-01-02

The General Medical Council (GMC) conducts Tests of Competence (ToC) for doctors referred for Fitness to Practise (FtP) issues. GPs take a single best answer knowledge test, an Objective Structured Clinical Examination (OSCE), and a Simulated Surgery (SimSurg) assessment which is a simulated GP consultation. The aim of this study was to examine the similarities between OSCEs and SimSurg to determine whether each assessment contributed something unique to GP ToCs. A mixed methods approach was used. Data were collated on 153 GPs who were required to undertake a ToC as a part of being investigated for FtP issues between February 2010 and October 2016. Using correlation analysis, we examined to what degree performance on the knowledge test, OSCE, and SimSurg related to case examiner recommendations and FtP outcomes, including the unique predictive power of these three assessments. The outcome measures were case examiner recommendations (i) not fit to practise; ii) fit to practise on a limited basis; or iii) fit to practise) as well as FtP outcomes (i) erased/removed from the register; ii) having restrictions/conditions; or iii) be in good standing). For the qualitative component, 45 GP assessors were asked to rate whether they assess the same competencies and which assessment provides better feedback about candidates. There was significant overlap between OSCEs and SimSurg, p < 0.001. SimSurg had additional predictive power in the presence of OSCEs and the knowledge test (p = 0.030) in distinguishing doctors from different FtP categories, while OSCEs did not (p = 0.080). Both the OSCEs (p = 0.004) and SimSurg (p < 0.001) had significant negative correlations with case examiner recommendations when accounting for the effects of the other two assessments. Inductive thematic analysis of the responses to the questionnaire showed that assessors perceived OSCEs to be better suited to target specific knowledge and skills. SimSurg was thought to produce a more global picture as the scenarios more accurately portray a patient consultation. While all three assessments are strong predictors of both case examiner recommendations and FtP outcomes, our findings suggest that the efficiency of GP ToCs can be improved by removing some of this overlapping content.

AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway

PubMed Central

Chen, Ying-Hua; Chen, Zhang-Wei; Li, Hong-Mei

2018-01-01

Objective Diabetes is associated with accelerated formation of advanced glycation end products (AGEs) that are extensively found in circulating endothelial microparticles (EMPs). This study aimed to investigate whether AGEs have a direct effect on EMP formation and the possible underlying mechanism. Methods In vitro, cultured human umbilical vein endothelial cells (HUVECs) were incubated with AGEs (200 and 400 μg/ml) for 24 hours with or without pretreatment with anti-RAGE antibody, NOX inhibitor, or ROS scavenger. The number of CD31-positive EMPs was assessed by flow cytometry. Results The number of EMPs was significantly increased in HUVECs stimulated by AGEs in a dose-dependent manner. In addition, receptors for AGEs (RAGE), NAD(P)H oxidase (NOX), and reactive oxygen species (ROS) were increased by AGEs as compared to the control group. These changes could be reversed when HUVECs were pretreated with anti-RAGE antibody. Moreover, inhibition of NOX as well as antioxidant treatment reduced the release of EMPs induced by AGEs. Conclusion Our study suggested that AGEs increased EMP generation, which was mediated by RAGE signaling through NOX-derived ROS. PMID:29744367

AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway.

PubMed

Chen, Ying-Hua; Chen, Zhang-Wei; Li, Hong-Mei; Yan, Xin-Feng; Feng, Bo

2018-01-01

Diabetes is associated with accelerated formation of advanced glycation end products (AGEs) that are extensively found in circulating endothelial microparticles (EMPs). This study aimed to investigate whether AGEs have a direct effect on EMP formation and the possible underlying mechanism. In vitro, cultured human umbilical vein endothelial cells (HUVECs) were incubated with AGEs (200 and 400  μ g/ml) for 24 hours with or without pretreatment with anti-RAGE antibody, NOX inhibitor, or ROS scavenger. The number of CD31-positive EMPs was assessed by flow cytometry. The number of EMPs was significantly increased in HUVECs stimulated by AGEs in a dose-dependent manner. In addition, receptors for AGEs (RAGE), NAD(P)H oxidase (NOX), and reactive oxygen species (ROS) were increased by AGEs as compared to the control group. These changes could be reversed when HUVECs were pretreated with anti-RAGE antibody. Moreover, inhibition of NOX as well as antioxidant treatment reduced the release of EMPs induced by AGEs. Our study suggested that AGEs increased EMP generation, which was mediated by RAGE signaling through NOX-derived ROS.

Sensitivity of worst-case strom surge considering influence of climate change

NASA Astrophysics Data System (ADS)

Takayabu, Izuru; Hibino, Kenshi; Sasaki, Hidetaka; Shiogama, Hideo; Mori, Nobuhito; Shibutani, Yoko; Takemi, Tetsuya

2016-04-01

There are two standpoints when assessing risk caused by climate change. One is how to prevent disaster. For this purpose, we get probabilistic information of meteorological elements, from enough number of ensemble simulations. Another one is to consider disaster mitigation. For this purpose, we have to use very high resolution sophisticated model to represent a worst case event in detail. If we could use enough computer resources to drive many ensemble runs with very high resolution model, we can handle these all themes in one time. However resources are unfortunately limited in most cases, and we have to select the resolution or the number of simulations if we design the experiment. Applying PGWD (Pseudo Global Warming Downscaling) method is one solution to analyze a worst case event in detail. Here we introduce an example to find climate change influence on the worst case storm-surge, by applying PGWD to a super typhoon Haiyan (Takayabu et al, 2015). 1 km grid WRF model could represent both the intensity and structure of a super typhoon. By adopting PGWD method, we can only estimate the influence of climate change on the development process of the Typhoon. Instead, the changes in genesis could not be estimated. Finally, we drove SU-WAT model (which includes shallow water equation model) to get the signal of storm surge height. The result indicates that the height of the storm surge increased up to 20% owing to these 150 years climate change.

Constraints on Nubular Electromagnetic Pulses

NASA Astrophysics Data System (ADS)

Eisenhour, D. D.; Buseck, P. R.

1993-07-01

Chondritic meteorites contain an abundance of silicate minerals with opaque inclusions of oxides, sulfides, and metals. These host silicates interact differently from their enclosed opaques to electromagnetic (EM) radiation; specifically, silicates are inefficient at absorbing EM energy in the visible and near infrared while metals, sulfides, and Fe oxides absorb strongly in this frequency range. In the presence of a strong electromagnetic pulse (EMP), this preferential absorption leads to the selective heating of the opaque inclusions and can produce unique textures ("dirty snowballs": intimate, ~spherical intergrowths of silicate and opaque minerals with radii of < 1 to 10 micrometers) that record the passage of the EMP. Many chondrules, CAIs, and isolated silicate grains within chondritic meteorites exhibit these unique features, suggesting that strong EMPs were common in the early solar nebula [1]. Here we discuss new constraints on nebular EMPs obtained from both experimental simulations and calculations of radiative heat transport. To test the feasibility of producing "dirty snowball" textures by EMP heating, olivines and pyroxenes containing metal and sulfide inclusions were heated with a 10 watt, argon-ion, CW laser operated at 514 nm. Comparisons between meteoritic "dirty snowball" textures and experimentally produced textures confirm the ability to produce the meteoritic textures by EMP heating and suggest heating times and fluxes of 0.25 to 10 seconds and 10^9 to 10^10 ergs cm^-2 sec^-1. Fluxes less than 10^9 ergs cm^-2 sec^-1 were insufficient to melt metal and sulfide inclusions, while fluxes greater than 10^10 ergs cm^-2 sec^-1 resulted in complete melting of metal, sulfide, and silicates. The experimentally determined heating time scales suggest that radiative equilibrium was reached in the "dirty snowball" formation process, indicating that the range of observed textures is controlled by cooling rates. Calculations of radiative absorption and emission allow further constraints to be placed on the EMPs responsible for "dirty snowball" formation. The absorption and emission efficiencies of grains in a blackbody radiation field were determined by calculating Planck mean cross sections for olivine, pyroxene, and iron as a function of grain size [2,3]. This information was combined with conductive heat flow calculations to determine the behavior of olivine and pyroxene grains with small inclusions of metal. Results indicate that "dirty snowball" formation results only over a narrow flux range for a given multiphase assemblage, with higher fluxes required for smaller, more transparent, or more refractory grains. For a 100-mm olivine chondrule containing a 10-micrometer "dirty snowball," the required flux is ~9 +- 1 x 10^8 ergs cm^-2 sec^-1, with a minimum pulse duration of 4 seconds (assuming an initial grain temperature of 500 K prior to heating). These values are in good agreement with experimentally determined values. The results show that pulses energetic enough to create "dirty snowballs" are also capable of producing the total melting required for chondrule formation with only slight increases in flux, or with only marginally different grain properties (e.g., more opaque inclusions, lower melting points, higher absorption cross sections). Because of the temperature and grain size dependence of the Planck mean cross sections of silicates, an EMP of the type described above will selectively melt larger aggregates and individual grains (>100 micrometer) while leaving smaller aggregates and grains unmelted. Therefore, natural products of EMP heating are: 1) the formation of chondrules in a sustained dusty environment, 2) a paucity of small chondrules, and 3) residual grains relatively unaffected by the EMPs. References: [1] Eisenhour D. D. and Buseck P. R. (1993) LPSC XXIV, 435-436. [2] Falk S. W. and Scalo J. M. (1975) Ap. J., 202, 690-695. [3] Gilman R. C. (1974) Ap. J. Supp., 268, 28, 397-403.

Perform Experiments on LINUS-O and LTX Imploding Liquid Liner Fusion Systems.

DTIC Science & Technology

1982-08-27

EXPERIMENTS .. .. .. ... 3 III. HOMOPOLAR GENERATOR/INDUCTOR POWER SUPPLY EXPERIMENTS. 11 IV. PLASMA SWITCH EXPERIMENTS. .. .. .. .... . ..... 18 V... homopolar generator (HPG) inductive load system. 0 Conduct an electromagnetic pulse (EMP) simulation demonstration using the NRL HPG/inductive storage...suggest solutions to the unstable flow problem, the research was suspended due to the program redirection. -10- IT III. HOMOPOLAR GENERATOR/INDUCTOR POWER

A Theory of Electromagnetic Shielding with Applications to MIL-STD-285, IEEE-299, and EMP Simulation

DTIC Science & Technology

1985-02-01

in a building sized enclosure slot-like discontinuities may not all be small compar- ed to all wavelengths in the incident field, and slot resonan ...OFFICE OF RESEARCH/ NPP US AIR FORCE SPACE COMMAND ATTN STATE & LOCAL PROG SUPPORT O ATTN KKO 500 C STREET, SW ATTN KRQ WASHINGTON, DC 20472 ATTN XPOW

Mapping the transcription start points of the Staphylococcus aureus eap, emp, and vwb promoters reveals a conserved octanucleotide sequence that is essential for expression of these genes.

PubMed

Harraghy, Niamh; Homerova, Dagmar; Herrmann, Mathias; Kormanec, Jan

2008-01-01

Mapping the transcription start points of the eap, emp, and vwb promoters revealed a conserved octanucleotide sequence (COS). Deleting this sequence abolished the expression of eap, emp, and vwb. However, electrophoretic mobility shift assays gave no evidence that this sequence was a binding site for SarA or SaeR, known regulators of eap and emp.

Extramedullary Solitary Plasmacytoma: Demonstrating the Role of 18F-FDG PET Imaging.

PubMed

Gautam, Archana; Sahu, Kamal Kant; Alamgir, Ahsan; Siddiqi, Imran; Ailawadhi, Sikander

2017-04-01

An Extramedullary Plasmacytoma (EMP) is characterized by a neoplastic proliferation of clonal plasma cells outside the medullary cavity. EMPs are a rare occurrence compared to other malignant plasma cell disorders and account for approximately 3-5% of plasma-cell neoplasms. Although most cases of EMP are not immediately life threatening at diagnosis, EMPs can progress to Multiple Myeloma (MM) and thus, warrant monitoring. Currently, there are no standard guidelines for when and how to monitor patients who are diagnosed with or treated for a solitary plasmacytoma. We present a case of solitary EMP who was treated adequately and definitively but developed a distinct, non-contiguous subsequent solitary EMP and was only discovered due to surveillance 18 F-Fludeoxyglucose Positron Emission Tomography ( 18 F-FDG) (PET) scan. Uniform surveillance guidelines should be developed and the potential benefits of PET and other imaging techniques as well as their cost should be considered.

Extramedullary Solitary Plasmacytoma: Demonstrating the Role of 18F-FDG PET Imaging

PubMed Central

Gautam, Archana; Sahu, Kamal Kant; Alamgir, Ahsan; Siddiqi, Imran

2017-01-01

An Extramedullary Plasmacytoma (EMP) is characterized by a neoplastic proliferation of clonal plasma cells outside the medullary cavity. EMPs are a rare occurrence compared to other malignant plasma cell disorders and account for approximately 3-5% of plasma-cell neoplasms. Although most cases of EMP are not immediately life threatening at diagnosis, EMPs can progress to Multiple Myeloma (MM) and thus, warrant monitoring. Currently, there are no standard guidelines for when and how to monitor patients who are diagnosed with or treated for a solitary plasmacytoma. We present a case of solitary EMP who was treated adequately and definitively but developed a distinct, non-contiguous subsequent solitary EMP and was only discovered due to surveillance 18F-Fludeoxyglucose Positron Emission Tomography (18F-FDG) (PET) scan. Uniform surveillance guidelines should be developed and the potential benefits of PET and other imaging techniques as well as their cost should be considered. PMID:28571247

Investigation into the electromagnetic impulses from long-pulse laser illuminating solid targets inside a laser facility

NASA Astrophysics Data System (ADS)

Yi, Tao; Yang, Jinwen; Yang, Ming; Wang, Chuanke; Yang, Weiming; Li, Tingshuai; Liu, Shenye; Jiang, Shaoen; Ding, Yongkun; Xiao, Shaoqiu

2016-09-01

Emission of the electromagnetic pulses (EMP) due to laser-target interaction in laser facility had been evaluated using a cone antenna in this work. The microwave in frequencies ranging from several hundreds of MHz to 2 GHz was recorded when long-pulse lasers with several thousands of joules illuminated the solid targets, meanwhile the voltage signals from 1 V to 4 V were captured as functions of laser energy and backlight laser, where the corresponding electric field strengths were obtained by simulating the cone antenna in combination with conducting a mathematical process (Tiknohov Regularization with L curve). All the typical coupled voltage oscillations displayed multiple peaks and had duration of up to 80 ns before decaying into noise and mechanisms of the EMP generation was schematically interpreted in basis of the practical measuring environments. The resultant data were expected to offer basic know-how to achieve inertial confinement fusion.

Synthetic gelatinases inhibitor attenuates electromagnetic pulse-induced blood-brain barrier disruption by inhibiting gelatinases-mediated ZO-1 degradation in rats.

PubMed

Qiu, Lian-Bo; Zhou, Yan; Wang, Qi; Yang, Long-Long; Liu, Hai-Qiang; Xu, Sheng-Long; Qi, Yu-Hong; Ding, Gui-Rong; Guo, Guo-Zhen

2011-07-11

Previously we found that exposure to electromagnetic pulse (EMP) induced an increase in blood-brain-barrier (BBB) permeability and the degradation of tight junction protein ZO-1 in rats. Matrix metalloproteinases (MMPs), in particular gelatinases (MMP-2 and MMP-9), play a key role in degradation of tight junction proteins, are known mediators of BBB compromise. We hypothesized that the degradation of ZO-1 by gelatinases contributed to EMP-induced BBB opening. To test this hypothesis, the mRNA level of ZO-1, protein levels of MMP-2, MMP-9 and tissue inhibitor of metalloproteinases (TIMP-1 and TIMP-2) were detected in rat cerebral cortex after exposing rats to EMP at 200 kV/m for 200 pulses. It was found that the mRNA level of ZO-1 was unaltered at different time points after EMP exposure. The protein levels of MMP-2 and MMP-9 significantly increased at 3 h and 0.5 h, respectively. However, TIMP-1 (inhibitor of MMP-9) and TIMP-2 (inhibitor of MMP-2) only moderately increased after EMP exposure. In addition, in situ zymography results showed that the gelatinase activity increased in cerebral microvessels at 3 h after EMP exposure. When rats were treated with gelatinases inhibitor (SB-3CT) before EMP exposure, the EMP-induced BBB opening was attenuated and the ZO-1 degradation was reversed. Our results suggested that EMP-induced BBB opening was related to gelatinase mediated ZO-1 degradation. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

The Prognostic Value of Epithelial Membrane Protein 1 (EMP-1) in Patients with Laryngeal Carcinoma

PubMed Central

Liu, Chang; Wei, Xiaojun; Li, Feng; Wang, Li; Ruan, Xinjian; Jia, Jia; Zhang, Xia

2017-01-01

Background In the present study, we aimed to investigate the prognostic value of epithelial membrane protein 1 (EMP-1) gene in patients diagnosed with laryngeal carcinoma (LC). Material/Methods Patients who were pathologically diagnosed with LC were enrolled in the present study. The expression levels of EMP-1 in tumor tissues and corresponding normal tissues collected from the LC patients were detected by semi-reverse transcriptase polymerase chain reaction (semi-RT-PCR). Chi-square analysis was used to evaluate the relationship between EMP-1 expression level and clinical characteristics. Survival analysis for the study population was analyzed by Kaplan-Meier method with log rank test. Additionally, Cox regression model was applied to evaluate the prognostic value of EMP-1 in LC patients. Results 106 LC patients, including 55 men and 51 women, were enrolled in the present study. Semi-RT-PCR demonstrated that the expression level of EMP-1 was decreased in tumor tissues, compared with adjacent normal tissues (p<0.001). Moreover, the level was significantly associated with lymph node metastasis, histological grade, and clinical stage (p<0.05 for all). In addition, low levels of EMP-1 was significantly correlated with poor survival rate (log rank test, p=0.020). Cox regression analysis indicated that EMP-1 was an independent marker for LC prognosis (HR=2.755, 95% CI=1.123–6.760, p=0.027). Conclusions The abnormal expression of EMP-1 may be associated with progression of LC and the gene may act as a prognostic marker for LC. PMID:28779068

A mutational approach for the detection of genetic factors affecting seed size in maize.

PubMed

Sangiorgio, Stefano; Carabelli, Laura; Gabotti, Damiano; Manzotti, Priscilla Sofia; Persico, Martina; Consonni, Gabriella; Gavazzi, Giuseppe

2016-12-01

Genes influencing seed size. The designation emp (empty pericarp) refers to a group of defective kernel mutants that exhibit a drastic reduction in endosperm tissue production. They allow the isolation of genes controlling seed development and affecting seed size. Nine independently isolated emp mutants have been analyzed in this study and in all cases longitudinal sections of mature seeds revealed the absence of morphogenesis in the embryo proper, an observation that correlates with their failure to germinate. Complementation tests with the nine emp mutants, crossed inter se in all pairwise combinations, identified complementing and non-complementing pairs in the F 1 progenies. Data were then validated in the F 2 /F 3 generations. Mutant chromosomal location was also established. Overall our study has identified two novel emp genes and a novel allele at the previously identified emp4 gene. The introgression of single emp mutants in a different genetic background revealed the existence of a cryptic genetic variation (CGV) recognizable as a variable increase in the endosperm tissue. The unmasking of CGV by introducing single mutants in different genetic backgrounds is the result of the interaction of the emp mutants with a suppressor that has no obvious phenotype of its own and is present in the genetic background of the inbred lines into which the emp mutants were transferred. On the basis of these results, emp mutants could be used as tools for the detection of genetic factors that enhance the amount of endosperm tissue in the maize kernel and which could thus become valuable targets to exploit in future breeding programs.

Detrimental effect of electromagnetic pulse exposure on permeability of in vitro blood-brain-barrier model.

PubMed

Zhou, Jia Xing; Ding, Gui Rong; Zhang, Jie; Zhou, Yong Chun; Zhang, Yan Jun; Guo, Guo Zhen

2013-02-01

To study the effect of electromagnetic pulse (EMP) exposure on permeability of in vitro blood-brain-barrier (BBB) model. An in vitro BBB model, established by co-culturing brain microvascular endothelial cells (BMVEC) and astroglial cells (AC) isolated from rat brain, was exposed to EMP at 100 kV/m and 400 kV/m, respectively. Permeability of the model was assayed by measuring the transendothelial electrical resistance (TEER) and the horseradish peroxidase (HRP) transmission at different time points. Levels of BBB tight junction-related proteins were measured at 0, 1, 2, 4, 8, 12, 16, 20, 24 h after EMP exposure by Western blotting. The TEER level was lower in BBB model group than in control group at 12 h after EMP, exposure which returned to its normal level at 24 h. The 24 h recovery process was triphasic and biphasic respectively after EMP exposure at 100 kV/m and 400 kV/m. Following exposure to 400 kV/m EMP, the HRP permeability increased at 1-12 h and returned to its normal level at 24 h. Western blotting showed that the claudin-5 and ZO-1 protein levels were changed after EMP exposure. EMP exposure at 100 kV/m and 400 kV/m can increase the permeability of in vitro BBB model and BBB tight junction-related proteins such as ZO-1 and claudin-5 may change EMP-induced BBB permeability. Copyright © 2013 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

The Prognostic Value of Epithelial Membrane Protein 1 (EMP-1) in Patients with Laryngeal Carcinoma.

PubMed

Liu, Chang; Wei, Xiaojun; Li, Feng; Wang, Li; Ruan, Xinjian; Jia, Jia; Zhang, Xia

2017-08-05

BACKGROUND In the present study, we aimed to investigate the prognostic value of epithelial membrane protein 1 (EMP-1) gene in patients diagnosed with laryngeal carcinoma (LC). MATERIAL AND METHODS Patients who were pathologically diagnosed with LC were enrolled in the present study. The expression levels of EMP-1 in tumor tissues and corresponding normal tissues collected from the LC patients were detected by semi-reverse transcriptase polymerase chain reaction (semi-RT-PCR). Chi-square analysis was used to evaluate the relationship between EMP-1 expression level and clinical characteristics. Survival analysis for the study population was analyzed by Kaplan-Meier method with log rank test. Additionally, Cox regression model was applied to evaluate the prognostic value of EMP-1 in LC patients. RESULTS 106 LC patients, including 55 men and 51 women, were enrolled in the present study. Semi-RT-PCR demonstrated that the expression level of EMP-1 was decreased in tumor tissues, compared with adjacent normal tissues (p<0.001). Moreover, the level was significantly associated with lymph node metastasis, histological grade, and clinical stage (p<0.05 for all). In addition, low levels of EMP-1 was significantly correlated with poor survival rate (log rank test, p=0.020). Cox regression analysis indicated that EMP-1 was an independent marker for LC prognosis (HR=2.755, 95% CI=1.123-6.760, p=0.027). CONCLUSIONS The abnormal expression of EMP-1 may be associated with progression of LC and the gene may act as a prognostic marker for LC.

Efficacy of estramustine phosphate sodium hydrate (EMP) monotherapy in castration-resistant prostate cancer patients: report of 102 cases and review of literature.

PubMed

Matsumoto, Kazuhiro; Tanaka, Nobuyuki; Hayakawa, Nozomi; Ezaki, Taisuke; Suzuki, Kenjiro; Maeda, Takahiro; Ninomiya, Akiharu; Nakamura, So

2013-12-01

This retrospective chart review study was conducted to evaluate the efficacy of estramustine phosphate sodium hydrate (EMP) monotherapy in patients with castration-resistant prostate cancer (CRPC) and to determine who would benefit from EMP therapy. EMP was administered at a daily dose of 560 mg to 102 patients as a third-line therapy, who had already received combined androgen blockade (CAB) and subsequent alternative antiandrogen therapy. The responses to EMP after its induction and its toxicity were evaluated. We also analyzed the association between the clinicopathological factors of the patients and their responses to EMP therapy. A reduction in the serum prostate-specific antigen (PSA) 4 weeks after induction was observed in 70 patients (68.6%), while 30 cases (29.4%) achieved more than 50% reduction of PSA. Long-term reduction of PSA from baseline for more than 6 months was observed in 31 patients (30.4%). EMP treatment was discontinued in 11 patients (10.8%) because of side effects (nausea in six patients, gynecomastia in three patients, eruption in one patient, and liver dysfunction in one patient). Multivariate analysis demonstrated that long duration of prior hormonal therapy was an independent favorable factor for reduced PSA levels, long responses, and overall survival. The data suggest that oral EMP administration as a third-line monotherapy is well tolerated and effective to some degree in patients with CRPC who have already received CAB and subsequent alternative antiandrogen therapy. Thus, EMP can be regarded as one treatment option, especially for patients whose prior duration of hormonal therapy was long.

Catabolic Effects of Endothelial Cell-Derived Microparticles on Disc Cells: Implications in Intervertebral Disc Neovascularization and Degeneration

PubMed Central

Pohl, Pedro H. I.; Lozito, Thomas P.; Cuperman, Thais; Yurube, Takashi; Moon, Hong J.; Ngo, Kevin; Tuan, Rocky S.; Croix, Claudette St.; Sowa, Gwendolyn A.; Rodrigues, Luciano M. R.; Kang, James D.; Vo, Nam V.

2017-01-01

Neovascularization of intervertebral discs, a phenomenon considered pathological since normal discs are primarily avascular structures, occurs most frequently in annulus fibrosus (AF) of degenerated discs. Endothelial cells (ECs) are involved in this process, but the mechanism of the interaction between AF and endothelial cells is unclear. In this study we evaluated the effects on matrix catabolic activity of AF cells by the extracellular endothelial microparticles (EMPs) and soluble protein factors (SUP fraction) produced from ECs. Passage 1 human AF cells grown in monolayer cultures were treated for 72 hours with 250μg of EMPs or SUP fraction isolated from culture of the microvascular endothelial cell line, HEMC-I. Live-cell imaging revealed uptake of EMPs by AF cells. RT-PCR analysis demonstrated increased mRNA expression of MMP-1 (50.3 fold), MMP-3 (4.5 fold) and MMP-13 (5.5 fold) in AF cell cultures treated with EMPs compared to untreated control. Western analysis also demonstrated increased MMP protein expression in EMP-treated AF cells. AF cells treated with the SUP fraction also exhibited a dramatic increase in MMP mRNA and protein expression. Increased MMP expression is primarily due to EMP or SUP stimulation of AF cells since EMPs or SUP fraction alone contained negligible amount of MMPs. Interestingly, MMP activity was elevated in AF cell cultures treated with EMPs but not with SUP. This study revealed enhanced matrix catabolism as a molecular consequence of action of ECs on AF cells via EMPs, which might be expected during neo-angiogenesis of degenerating disc. PMID:27246627

Maximum wind radius estimated by the 50 kt radius: improvement of storm surge forecasting over the western North Pacific

NASA Astrophysics Data System (ADS)

Takagi, Hiroshi; Wu, Wenjie

2016-03-01

Even though the maximum wind radius (Rmax) is an important parameter in determining the intensity and size of tropical cyclones, it has been overlooked in previous storm surge studies. This study reviews the existing estimation methods for Rmax based on central pressure or maximum wind speed. These over- or underestimate Rmax because of substantial variations in the data, although an average radius can be estimated with moderate accuracy. As an alternative, we propose an Rmax estimation method based on the radius of the 50 kt wind (R50). Data obtained by a meteorological station network in the Japanese archipelago during the passage of strong typhoons, together with the JMA typhoon best track data for 1990-2013, enabled us to derive the following simple equation, Rmax = 0.23 R50. Application to a recent strong typhoon, the 2015 Typhoon Goni, confirms that the equation provides a good estimation of Rmax, particularly when the central pressure became considerably low. Although this new method substantially improves the estimation of Rmax compared to the existing models, estimation errors are unavoidable because of fundamental uncertainties regarding the typhoon's structure or insufficient number of available typhoon data. In fact, a numerical simulation for the 2013 Typhoon Haiyan as well as 2015 Typhoon Goni demonstrates a substantial difference in the storm surge height for different Rmax. Therefore, the variability of Rmax should be taken into account in storm surge simulations (e.g., Rmax = 0.15 R50-0.35 R50), independently of the model used, to minimize the risk of over- or underestimating storm surges. The proposed method is expected to increase the predictability of major storm surges and to contribute to disaster risk management, particularly in the western North Pacific, including countries such as Japan, China, Taiwan, the Philippines, and Vietnam.

77 FR 27178 - Notice of Funds Availability; Inviting Applications for the Emerging Markets Program; Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-09

... for the Emerging Markets Program (EMP). The document contained incorrect dates. FOR FURTHER... Times. EMP funding is reviewed on a rolling basis during the fiscal year as long as EMP funding is...

Blockade of epithelial membrane protein 2 (EMP2) abrogates infection of Chlamydia muridarum murine genital infection model

PubMed Central

Shimazaki, Kaori; Chan, Ann M.; Moniz, Raymond J.; Wadehra, Madhuri; Nagy, Agnes; Coulam, C. Paige; Mareninov, Sergey; Lepin, Eric M.; Wu, Anna M.; Kelly, Kathleen A.; Braun, Jonathan; Gordon, Lynn K.

2012-01-01

New methods are needed to eradicate or prevent Chlamydia trachomatis infections. Blockade of epithelial membrane protein 2 (EMP2) by genetic silencing or neutralizing polyclonal antibody reduced chlamydial infectivity in vitro. This study tests the prediction that recombinant anti-EMP2 diabody could reduce early chlamydial infection of the genital tract in vivo. In a murine infection model, pretreatment with anti-EMP2 diabody, as compared to control diabody, significantly reduced bacterial load, tissue production of inflammatory cytokines, recruitment of polymorphonuclear leukocytes, and local tissue inflammation. These findings support EMP2 as a potential preventative and therapeutic target for genital chlamydial infection. PMID:19159428

East China Sea Storm Surge Modeling and Visualization System: the Typhoon Soulik case.

PubMed

Deng, Zengan; Zhang, Feng; Kang, Linchong; Jiang, Xiaoyi; Jin, Jiye; Wang, Wei

2014-01-01

East China Sea (ECS) Storm Surge Modeling System (ESSMS) is developed based on Regional Ocean Modeling System (ROMS). Case simulation is performed on the Typhoon Soulik, which landed on the coastal region of Fujian Province, China, at 6 pm of July 13, 2013. Modeling results show that the maximum tide level happened at 6 pm, which was also the landing time of Soulik. This accordance may lead to significant storm surge and water level rise in the coastal region. The water level variation induced by high winds of Soulik ranges from -0.1 to 0.15 m. Water level generally increases near the landing place, in particular on the left hand side of the typhoon track. It is calculated that 0.15 m water level rise in this region can cause a submerge increase of ~0.2 km(2), which could be catastrophic to the coastal environment and the living. Additionally, a Globe Visualization System (GVS) is realized on the basis of World Wind to better provide users with the typhoon/storm surge information. The main functions of GVS include data indexing, browsing, analyzing, and visualization. GVS is capable of facilitating the precaution and mitigation of typhoon/storm surge in ESC in combination with ESSMS.

Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints

PubMed Central

Ulrich, Alexander; Andersen, Kasper R.; Schwartz, Thomas U.

2012-01-01

We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb. EMP further enables simultaneous cloning of multiple inserts. PMID:23300917

Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

PubMed

Ulrich, Alexander; Andersen, Kasper R; Schwartz, Thomas U

2012-01-01

We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb. EMP further enables simultaneous cloning of multiple inserts.

Interferon-β1a reduces plasma CD31+ endothelial microparticles (CD31+EMP) in multiple sclerosis

PubMed Central

Sheremata, William A; Jy, Wenche; Delgado, Sylvia; Minagar, Alireza; McLarty, Jerry; Ahn, Yeon

2006-01-01

Background A correlation between plasma CD31+ endothelial microparticles (CD31+EMP) levels and clinical, as well as brain MRI activity, in multiple sclerosis (MS) patients has been previously reported. However, the effect(s) of treatment with interferon-β1a (IFN-β1a) on plasma levels of CD31+EMP has not been assessed. In a prospective study, we measured plasma CD31+EMP levels in 30 patients with relapsing-remitting MS. Methods Using flow cytometry, in a blinded study, we measured plasma CD31+EMP in 30 consecutive patients with relapsing-remitting MS (RRMS) prior to and 4, 12, 24 and 52 weeks after initiation of intramuscular therapy with interferon-β1a (IFN-β1a), 30 micrograms weekly. At each visit, clinical examination was performed and expanded disability status scale (EDSS) scores were assessed. Results Plasma levels of CD31+EMP were significantly reduced from 24 through 52 weeks following initiation of treatment with IFN-β1a. Conclusion Our data suggest that serial measurement of plasma CD31+EMP levels may be used as a surrogate marker of response to therapy with INF-β1a. In addition, the decline in plasma levels of CD31+EMP further supports the concept that IFN-β1a exerts stabilizing effect on the cerebral endothelial cells in pathogenesis of MS. PMID:16952316

Interferon-beta1a reduces plasma CD31+ endothelial microparticles (CD31+EMP) in multiple sclerosis.

PubMed

Sheremata, William A; Jy, Wenche; Delgado, Sylvia; Minagar, Alireza; McLarty, Jerry; Ahn, Yeon

2006-09-04

A correlation between plasma CD31+ endothelial microparticles (CD31+EMP) levels and clinical, as well as brain MRI activity, in multiple sclerosis (MS) patients has been previously reported. However, the effect(s) of treatment with interferon-beta1a (IFN-beta1a) on plasma levels of CD31+EMP has not been assessed. In a prospective study, we measured plasma CD31+EMP levels in 30 patients with relapsing-remitting MS. Using flow cytometry, in a blinded study, we measured plasma CD31+EMP in 30 consecutive patients with relapsing-remitting MS (RRMS) prior to and 4, 12, 24 and 52 weeks after initiation of intramuscular therapy with interferon-beta1a (IFN-beta1a), 30 micrograms weekly. At each visit, clinical examination was performed and expanded disability status scale (EDSS) scores were assessed. Plasma levels of CD31+EMP were significantly reduced from 24 through 52 weeks following initiation of treatment with IFN-beta1a. Our data suggest that serial measurement of plasma CD31+EMP levels may be used as a surrogate marker of response to therapy with INF-beta1a. In addition, the decline in plasma levels of CD31+EMP further supports the concept that IFN-beta1a exerts stabilizing effect on the cerebral endothelial cells in pathogenesis of MS.

Clinical analysis of 40 multiple myeloma patients with extramedullary plasmacytoma of the head.

PubMed

Sun, Wan-Jun; Zhang, Jia-Jia; An, Na; Shen, Men; Huang, Zhong-Xia; Li, Xin

2016-12-01

Objectives To investigate the clinical characteristics, survival and prognosis of patients with multiple myeloma (MM) and head extramedullary plasmacytoma (EMP). Methods Forty MM patients were enrolled in the study (18 men, 22 women; median age, 55 years). Results Median overall survival (OS) and progression-free survival (PFS) were 24 (5-78) months and 17 (2-36) months, respectively. The 2-, 3- and 5-year OS rates were 51%, 20% and 7%, respectively. The 2-year PFS was 15%. Median OS and PFS in patients administered velcade were 26 (18-50) and 22.5 (5-78) months, compared with 20 (10-30) and 13.5 (2-36) months in patients without velcade, respectively. Median OS was 23.5 (5-50) months in patients with EMP at MM diagnosis ( n = 25) and 36 (22-78) months in patients with head EMP diagnosed during the disease course ( n = 15). Sixteen MM patients had EMP invasion of the head only and 24 had invasion at multiple sites. Median OS was 25 (22-78) months in patients with EMP of the head only and 22 (5-78) months in patients with EMP invasion at multiple sites. Conclusion MM patients with head EMP show a more aggressive disease course and shorter OS and PFS. The prognosis of these patients is poor, especially in patients with head EMP at MM diagnosis, though combined chemotherapy and radiotherapy may prolong survival.

Effect of endothelin-1 and endothelin receptor blockade on the release of microparticles.

PubMed

Jung, Christian; Lichtenauer, Michael; Wernly, Bernhard; Franz, Marcus; Goebel, Bjoern; Rafnsson, Arnar; Figulla, Hans-Reiner; Pernow, John

2016-08-01

Increased levels of endothelial cell microparticles (EMP) are known to reflect endothelial dysfunction (ED). In diabetes mellitus type 2 (T2DM), the expression of endothelin (ET)-1 is increased. As treatment with an ET-1 antagonist significantly inhibited atherosclerosis in animal models, we sought to investigate whether treatment with ET-1 antagonists affects EMP levels in vitro and in vivo in patients with T2DM. In vitro study: Human umbilical vein endothelial cells (HUVEC) were stimulated with ET-1 alone and ET-1 in combination with a dual ET-A and ET-B endothelin receptor blocker. In vivo study: Patients with T2DM were randomized to treatment with the ET receptor antagonist bosentan or placebo. After 4 weeks, the patients were re-examined and blood samples were obtained. EMP counts in supernatants and plasma samples were determined using flow cytometry. In vitro study: In supernatants of ET-1-stimulated HUVECs, the increased release of EMP was reduced significantly by co-incubation with an ET-1 receptor antagonist (e.g. CD31+/CD42b-EMP decreased from 37·1% ± 2·8 to 31·5% ± 2·8 SEM, P = 0·0078). In vivo study: No changes in EMP levels in blood samples of patients with T2DM were found after 4 weeks of bosentan treatment (n = 36, P = ns). Our in vitro results suggest that ET-1 stimulates the release of EMP from HUVECs via a receptor-dependent mechanism. Co-incubation with an endothelin receptor blocker abolished ET-1-dependent EMP release. However, treatment with bosentan for 4 weeks failed to alter EMP levels in patients with T2DM. Other factors seem to have influenced EMP release in patients with T2DM independent of ET-1 receptor-mediated mechanisms. © 2016 Stichting European Society for Clinical Investigation Journal Foundation.

Analysis of gene expression profile induced by EMP-1 in esophageal cancer cells using cDNA Microarray

PubMed Central

Wang, Hai-Tao; Kong, Jian-Ping; Ding, Fang; Wang, Xiu-Qin; Wang, Ming-Rong; Liu, Lian-Xin; Wu, Min; Liu, Zhi-Hua

2003-01-01

AIM: To obtain human esophageal cancer cell EC9706 stably expressed epithelial membrane protein-1 (EMP-1) with integrated eukaryotic plasmid harboring the open reading frame (ORF) of human EMP-1, and then to study the mechanism by which EMP-1 exerts its diverse cellular action on cell proliferation and altered gene profile by exploring the effect of EMP-1. METHODS: The authors first constructed pcDNA3.1/myc-his expression vector harboring the ORF of EMP-1 and then transfected it into human esophageal carcinoma cell line EC9706. The positive clones were analyzed by Western blot and RT-PCR. Moreover, the cell growth curve was observed and the cell cycle was checked by FACS technique. Using cDNA microarray technology, the authors compared the gene expression pattern in positive clones with control. To confirm the gene expression profile, semi-quantitative RT-PCR was carried out for 4 of the randomly picked differentially expressed genes. For those differentially expressed genes, classification was performed according to their function and cellular component. RESULTS: Human EMP-1 gene can be stably expressed in EC9706 cell line transfected with human EMP-1. The authors found the cell growth decreased, among which S phase was arrested and G1 phase was prolonged in the transfected positive clones. By cDNA microarray analysis, 35 genes showed an over 2.0 fold change in expression level after transfection, with 28 genes being consistently up-regulated and 7 genes being down-regulated. Among the classified genes, almost half of the induced genes (13 out of 28 genes) were related to cell signaling, cell communication and particularly to adhesion. CONCLUSION: Overexpression of human EMP-1 gene can inhibit the proliferation of EC9706 cell with S phase arrested and G1 phase prolonged. The cDNA microarray analysis suggested that EMP-1 may be one of regulators involved in cell signaling, cell communication and adhesion regulators. PMID:12632483

Analysis of gene expression profile induced by EMP-1 in esophageal cancer cells using cDNA Microarray.

PubMed

Wang, Hai-Tao; Kong, Jian-Ping; Ding, Fang; Wang, Xiu-Qin; Wang, Ming-Rong; Liu, Lian-Xin; Wu, Min; Liu, Zhi-Hua

2003-03-01

To obtain human esophageal cancer cell EC9706 stably expressed epithelial membrane protein-1 (EMP-1) with integrated eukaryotic plasmid harboring the open reading frame (ORF) of human EMP-1, and then to study the mechanism by which EMP-1 exerts its diverse cellular action on cell proliferation and altered gene profile by exploring the effect of EMP-1. The authors first constructed pcDNA3.1/myc-his expression vector harboring the ORF of EMP-1 and then transfected it into human esophageal carcinoma cell line EC9706. The positive clones were analyzed by Western blot and RT-PCR. Moreover, the cell growth curve was observed and the cell cycle was checked by FACS technique. Using cDNA microarray technology, the authors compared the gene expression pattern in positive clones with control. To confirm the gene expression profile, semi-quantitative RT-PCR was carried out for 4 of the randomly picked differentially expressed genes. For those differentially expressed genes, classification was performed according to their function and cellular component. Human EMP-1 gene can be stably expressed in EC9706 cell line transfected with human EMP-1. The authors found the cell growth decreased, among which S phase was arrested and G1 phase was prolonged in the transfected positive clones. By cDNA microarray analysis, 35 genes showed an over 2.0 fold change in expression level after transfection, with 28 genes being consistently up-regulated and 7 genes being down-regulated. Among the classified genes, almost half of the induced genes (13 out of 28 genes) were related to cell signaling, cell communication and particularly to adhesion. Overexpression of human EMP-1 gene can inhibit the proliferation of EC9706 cell with S phase arrested and G1 phase prolonged. The cDNA microarray analysis suggested that EMP-1 may be one of regulators involved in cell signaling, cell communication and adhesion regulators.

EMP-1 is a junctional protein in a liver stem cell line and in the liver.

PubMed

Lee, Hsuan-Shu; Sherley, James L; Chen, Jeremy J W; Chiu, Chien-Chang; Chiou, Ling-Ling; Liang, Ja-Der; Yang, Pan-Chyr; Huang, Guan-Tarn; Sheu, Jin-Chuan

2005-09-09

In an attempt to discover cell markers for liver stem cells, a cDNA microarray analysis was carried out to compare the gene expression profiles between an adult liver stem cell line, Lig-8, and mature hepatocytes. Several genes in the categories of extracellular matrix, cell membrane, cell adhesion, transcription factor, signal molecule, transporter, and metabolic enzyme were shown to be differentially expressed in Lig-8 cells. Among them, epithelial membrane protein (EMP)-1 has been previously implicated with stem cell phenotypes. Antiserum to EMP-1 was produced to localize its expression. On monolayers of Lig-8 cells, EMP-1 was expressed along the intercellular border. In the liver harboring proliferating oval cells, the liver progenitors, EMP-1 was localized as ribbon bands, a staining pattern for epithelial junctions, all the way through bile duct epithelia, oval cell ductules, and into peri-hepatocytic regions. These peri-hepatocytic regions were proved to be bile canaliculi by co-localization of EMP-1 and dipeptidyl peptidase IV, an enzyme located on bile canaliculi. This report is the first to indicate EMP-1 to be a junctional protein in the liver.

Endothelial microparticles: Pathogenic or passive players in endothelial dysfunction in autoimmune rheumatic diseases?

PubMed

McCarthy, E M; Wilkinson, F L; Parker, B; Alexander, M Y

2016-11-01

Autoimmune rheumatic diseases are characterised by systemic inflammation and complex immunopathology, with an increased risk of cardiovascular disease, initiated by endothelial dysfunction in a chronic inflammatory environment. Endothelial microparticles (EMPs) are released into the circulation from activated endothelial cells and may therefore, reflect disease severity, vascular and endothelial dysfunction, that could influence disease pathogenesis via autocrine/paracrine signalling. The exact function of EMPs in rheumatic disease remains unknown, and this has initiated research to elucidate EMP composition and function, which may be determined by the mode of endothelial activation and the micro environment. To date, EMPs are thought to play a role in angiogenesis, thrombosis and inflammation by transferring specific proteins and microRNAs (miRs) to target cells. Here, we review the mechanisms underlying the generation and composition of EMPs and the clinical and experimental studies describing the involvement of EMPs in rheumatic diseases, since we have previously shown endothelial dysfunction and an elevated risk of cardiovascular disease are characteristics in systemic lupus erythematosus. We will also discuss the potential of EMPs as future biomarkers of cardiovascular risk in these diseases. Copyright © 2016 Elsevier Inc. All rights reserved.

Probabilistic Storm Surge Forecast For Venice

NASA Astrophysics Data System (ADS)

Mel, Riccardo; Lionello, Piero

2013-04-01

This study describes an ensemble storm surge prediction procedure for the city of Venice, which is potentially very useful for its management, maintenance and for operating the movable barriers that are presently being built. Ensemble Prediction System (EPS) is meant to complement the existing SL forecast system by providing a probabilistic forecast and information on uncertainty of SL prediction. The procedure is applied to storm surge events in the period 2009-2010 producing for each of them an ensemble of 50 simulations. It is shown that EPS slightly increases the accuracy of SL prediction with respect to the deterministic forecast (DF) and it is more reliable than it. Though results are low biased and forecast uncertainty is underestimated, the probability distribution of maximum sea level produced by the EPS is acceptably realistic. The error of the EPS mean is shown to be correlated with the EPS spread. SL peaks correspond to maxima of uncertainty and uncertainty increases linearly with the forecast range. The quasi linear dynamics of the storm surges produces a modulation of the uncertainty after the SL peak with period corresponding to that of the main Adriatic seiche.

F14A System Safety Program Plan

DTIC Science & Technology

1981-09-03

by block number) Electromagnetic Pulse (EMP) Safety Plans Test Program EMP Testing F14 Aircraft Plans 20 ABSTRACT (Continue on reverse side if...compromising completion of the required experimental tasks. This document addresses the safety aspect of performing an Electromagnetic Pulse (EMP) test

Electromagnetic Pulse (EMP) Survivability of Telecommunications Assets

DTIC Science & Technology

1987-02-06

Program Approach ES-2 2-1 EMP Mitigation Program Approach 2-2 2-2 Approach To The Assessment Of EMP Effects On Networks 2-3 2-3 Zone-Boundary Model 2-5...2-4 Approach To The Assessment Of EMP Effects On Networks 2-6 4-1 Zone-Boundary Model And Communications Network Elements 4-1 4-2 Important Features...data and theoretical analyses to arrive at the conclusions presented in this report. TMTrademark of Western Electric Co., Inc. ES-4 - i S

Dose dependency of outcomes of intrapleural fibrinolytic therapy in new rabbit empyema models

PubMed Central

Florova, Galina; Azghani, Ali O.; Buchanan, Ann; Boren, Jake; Allen, Timothy; Rahman, Najib M.; Koenig, Kathleen; Chamiso, Mignote; Karandashova, Sophia; Henry, James; Idell, Steven

2016-01-01

The incidence of empyema (EMP) is increasing worldwide; EMP generally occurs with pleural loculation and impaired drainage is often treated with intrapleural fibrinolytic therapy (IPFT) or surgery. A number of IPFT options are used clinically with empiric dosing and variable outcomes in adults. To evaluate mechanisms governing intrapleural fibrinolysis and disease outcomes, models of Pasteurella multocida and Streptococcus pneumoniae were generated in rabbits and the animals were treated with either human tissue (tPA) plasminogen activator or prourokinase (scuPA). Rabbit EMP was characterized by the development of pleural adhesions detectable by chest ultrasonography and fibrinous coating of the pleura. Similar to human EMP, rabbits with EMP accumulated sizable, 20- to 40-ml fibrinopurulent pleural effusions associated with extensive intrapleural organization, significantly increased pleural thickness, suppression of fibrinolytic and plasminogen-activating activities, and accumulation of high levels of plasminogen activator inhibitor 1, plasminogen, and extracellular DNA. IPFT with tPA (0.145 mg/kg) or scuPA (0.5 mg/kg) was ineffective in rabbit EMP (n = 9 and 3 for P. multocida and S. pneumoniae, respectively); 2 mg/kg tPA or scuPA IPFT (n = 5) effectively cleared S. pneumoniae-induced EMP collections in 24 h with no bleeding observed. Although intrapleural fibrinolytic activity for up to 40 min after IPFT was similar for effective and ineffective doses of fibrinolysin, it was lower for tPA than for scuPA treatments. These results demonstrate similarities between rabbit and human EMP, the importance of pleural fluid PAI-1 activity, and levels of plasminogen in the regulation of intrapleural fibrinolysis and illustrate the dose dependency of IPFT outcomes in EMP. PMID:27343192

MHD-EMP protection guidelines

NASA Astrophysics Data System (ADS)

Barnes, P. R.; Vance, E. F.

A nuclear detonation at altitudes several hundred kilometers above the earth will severely distort the earth's magnetic field and result in a strong magnetohydrodynamic electromagnetic pulse (MHD-EMP). The geomagnetic disturbance interacts with the soil to induce current and horizontal electric gradients. MHD-EMP, also called E3 since it is the third component of the high-altitude EMP (HEMP), lasts over 100 s after an exoatmospheric burst. MHD-EMP is similar to solar geomagnetic storms in it's global and low frequency (less than 1 Hz) nature except that E3 can be much more intense with a far shorter duration. When the MHD-EMP gradients are integrated over great distances by power lines, communication cables, or other long conductors, the induced voltages are significant. (The horizontal gradients in the soil are too small to induce major responses by local interactions with facilities.) The long pulse waveform for MHD-EMP-induced currents on long lines has a peak current of 200 A and a time-to-half-peak of 100 s. If this current flows through transformer windings, it can saturate the magnetic circuit and cause 60 Hz harmonic production. To mitigate the effects of MHD-EMP on a facility, long conductors must be isolated from the building and the commercial power harmonics and voltage swings must be addressed. The transfer switch would be expected to respond to the voltage fluctuations as long as the harmonics have not interfered with the switch control circuitry. The major sources of MHD-EMP induced currents are the commercial power lines and neutral; neutral current indirect coupling to the facility power or ground system via the metal fence, powered gate, parking lights, etc; metal water pipes; phone lines; and other long conductors that enter or come near the facility. The major source of harmonics is the commercial power system.

Endothelial microparticles interact with and support the proliferation of T cells.

PubMed

Wheway, Julie; Latham, Sharissa L; Combes, Valery; Grau, Georges E R

2014-10-01

Endothelial cells closely interact with circulating lymphocytes. Aggression or activation of the endothelium leads to an increased shedding of endothelial cell microparticles (MP). Endothelial MP (EMP) are found in high plasma levels in numerous immunoinflammatory diseases, such as atherosclerosis, sepsis, multiple sclerosis, and cerebral malaria, supporting their role as effectors and markers of vascular dysfunction. Given our recently described role for human brain microvascular endothelial cells (HBEC) in modulating immune responses, we investigated how HBEC-derived MP could interact with and support the proliferation of T cells. Like their mother cells, EMP expressed molecules important for Ag presentation and T cell costimulation, that is, β2-microglobulin, MHC II, CD40, and ICOSL. HBEC were able to take up fluorescently labeled Ags with EMP also containing fluorescent Ags, suggestive of Ag carryover from HBEC to EMP. In cocultures, fluorescently labeled EMP from resting or cytokine-stimulated HBEC formed conjugates with both CD4(+) and CD8(+) subsets, with higher proportions of T cells binding EMP from cytokine-stimulated cells. The increased binding of EMP from cytokinestimulated HBEC to T cells was VCAM-1 and ICAM-1 dependent. Finally, in CFSE T cell proliferation assays using anti-CD3 mAb or T cell mitogens, EMP promoted the proliferation of CD4(+) T cells and that of CD8(+) T cells in the absence of exogenous stimuli and in the T cell mitogenic stimulation. Our findings provide novel evidence that EMP can enhance T cell activation and potentially ensuing Ag presentation, thereby pointing toward a novel role for MP in neuroimmunological complications of infectious diseases. Copyright © 2014 by The American Association of Immunologists, Inc.

Shear stress regulates endothelial microparticle release.

PubMed

Vion, Anne-Clémence; Ramkhelawon, Bhama; Loyer, Xavier; Chironi, Gilles; Devue, Cecile; Loirand, Gervaise; Tedgui, Alain; Lehoux, Stéphanie; Boulanger, Chantal M

2013-05-10

Endothelial activation and apoptosis release membrane-shed microparticles (EMP) that emerge as important biological effectors. Because laminar shear stress (SS) is a major physiological regulator of endothelial survival, we tested the hypothesis that SS regulates EMP release. EMP levels were quantified by flow cytometry in medium of endothelial cells subjected to low or high SS (2 and 20 dyne/cm(2)). EMP levels augmented with time in low SS conditions compared with high SS conditions. This effect was sensitive to extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and Rho kinases inhibitors but unaffected by caspase inhibitors. Low SS-stimulated EMP release was associated with increased endothelial Rho kinases and ERK1/2 activities and cytoskeletal reorganization. Overexpression of constitutively active RhoA stimulated EMP release under high SS. We also examined the effect of nitric oxide (NO) in mediating SS effects. L-NG-nitroarginine methyl ester (L-NAME), but not D-NG-nitroarginine methyl ester, increased high SS-induced EMP levels by 3-fold, whereas the NO donor S-nitroso-N-acetyl-D,L-penicillamine (SNAP) decreased it. L-NAME and SNAP did not affect Rho kinases and ERK1/2 activities. Then, we investigated NO effect on membrane remodeling because microparticle release is abolished in ABCA1-deficient cells. ABCA1 expression, which was greater under low SS than under high SS, was augmented by L-NAME under high SS and decreased by SNAP under low SS conditions. Altogether, these results demonstrate that sustained atheroprone low SS stimulates EMP release through activation of Rho kinases and ERK1/2 pathways, whereas atheroprotective high SS limits EMP release in a NO-dependent regulation of ABCA1 expression and of cytoskeletal reorganization. These findings, therefore, identify endothelial SS as a physiological regulator of microparticle release.

Endothelial microparticles interact with and support the proliferation of T cells

PubMed Central

Wheway, Julie; Latham, Sharissa L; Combes, Valery; Grau, Georges ER

2014-01-01

Endothelial cells (EC) closely interact with circulating lymphocytes. Aggression or activation of the endothelium leads to an increased shedding of EC microparticles (MP). Endothelial MP (EMP) are found in high plasma levels in numerous immunoinflammatory diseases, e.g. atherosclerosis, sepsis, multiple sclerosis and cerebral malaria, supporting their role as effectors and markers of vascular dysfunction. Given our recently described role for human brain microvascular endothelial cells (HBEC) in modulating immune responses we investigated how HBEC-derived MP could interact with and support the proliferation of T cells. Like their mother cells, EMP expressed molecules important for antigen presentation and T cell co-stimulation, i.e., β2-microglobulin, MHC II, CD40 and ICOSL. HBEC were able to take up fluorescently labeled antigens with EMP also containing fluorescent antigens suggestive of antigen carryover from HBEC to EMP. In co-cultures, fluorescently labeled EMP from resting or cytokine-stimulated HBEC formed conjugates with both CD4+ and CD8+ subsets, with higher proportions of T cells binding EMP from cytokine stimulated cells. The increased binding of EMP from cytokine stimulated HBEC to T cells was VCAM-1 and ICAM-1-dependent. Finally, in CFSE T cell proliferation assays using anti-CD3 mAb or T cell mitogens, EMP promoted the proliferation of CD4+ T cells and that of CD8+ T cells in the absence of exogenous stimuli and in the T cell mitogenic stimulation. Our findings provide novel evidence that EMP can enhance T cell activation and potentially ensuing antigen presentation, thereby pointing towards a novel role for MP in neuro-immunological complications of infectious diseases. PMID:25187656

Dose dependency of outcomes of intrapleural fibrinolytic therapy in new rabbit empyema models.

PubMed

Komissarov, Andrey A; Florova, Galina; Azghani, Ali O; Buchanan, Ann; Boren, Jake; Allen, Timothy; Rahman, Najib M; Koenig, Kathleen; Chamiso, Mignote; Karandashova, Sophia; Henry, James; Idell, Steven

2016-08-01

The incidence of empyema (EMP) is increasing worldwide; EMP generally occurs with pleural loculation and impaired drainage is often treated with intrapleural fibrinolytic therapy (IPFT) or surgery. A number of IPFT options are used clinically with empiric dosing and variable outcomes in adults. To evaluate mechanisms governing intrapleural fibrinolysis and disease outcomes, models of Pasteurella multocida and Streptococcus pneumoniae were generated in rabbits and the animals were treated with either human tissue (tPA) plasminogen activator or prourokinase (scuPA). Rabbit EMP was characterized by the development of pleural adhesions detectable by chest ultrasonography and fibrinous coating of the pleura. Similar to human EMP, rabbits with EMP accumulated sizable, 20- to 40-ml fibrinopurulent pleural effusions associated with extensive intrapleural organization, significantly increased pleural thickness, suppression of fibrinolytic and plasminogen-activating activities, and accumulation of high levels of plasminogen activator inhibitor 1, plasminogen, and extracellular DNA. IPFT with tPA (0.145 mg/kg) or scuPA (0.5 mg/kg) was ineffective in rabbit EMP (n = 9 and 3 for P. multocida and S. pneumoniae, respectively); 2 mg/kg tPA or scuPA IPFT (n = 5) effectively cleared S. pneumoniae-induced EMP collections in 24 h with no bleeding observed. Although intrapleural fibrinolytic activity for up to 40 min after IPFT was similar for effective and ineffective doses of fibrinolysin, it was lower for tPA than for scuPA treatments. These results demonstrate similarities between rabbit and human EMP, the importance of pleural fluid PAI-1 activity, and levels of plasminogen in the regulation of intrapleural fibrinolysis and illustrate the dose dependency of IPFT outcomes in EMP. Copyright © 2016 the American Physiological Society.

Electromagnetic pulse activated brain microglia via the p38 MAPK pathway.

PubMed

Yang, Long-Long; Zhou, Yan; Tian, Wei-Dong; Li, Hai-Juan; Kang-Chu-Li; Miao, Xia; An, Guang-Zhou; Wang, Xiao-Wu; Guo, Guo-Zhen; Ding, Gui-Rong

2016-01-01

Previously, we found that electromagnetic pulses (EMP) induced an increase in blood brain barrier permeability and the leakage of albumin from blood into brain tissue. Albumin is known to activate microglia cells. Thus, we hypothesised that microglia activation could occur in the brain after EMP exposure. To test this hypothesis, the morphology and secretory function of microglia cells, including the expression of OX-42 (a marker of microglia activation), and levels of TNF-α, IL-10, IL-1β, and NO were determined in the rat cerebral cortex after EMP exposure. In addition, to examine the signalling pathway of EMP-induced microglia activation, protein and phosphorylated protein levels of p38, JNK and ERK were determined. It was found that the expression of OX-42increased significantly at 1, 6 and 12h (p<0.05) and recovered to the sham group level at 24h after EMP exposure. Levels of NO, TNF-α and IL-10 also changed significantly in vivo and in vitro after EMP exposure. The protein level of p38 and phosphorylated p38 increased significantly after EMP exposure (p<0.05) and recovered to sham levels at 12 and 24h, respectively. Protein and phosphorylated protein levels of ERK and JNK did not change. SB203580 (p38 inhibitor) partly prevented the change in NO, IL-10, IL-1β, TNF-α levels induced by EMP exposure. Taken together, these results suggested that EMP exposure (200kV/m, 200 pulses) could activate microglia in rat brain and affect its secretory function both in vivo and in vitro, and the p38 pathway is involved in this process. Copyright © 2015 Elsevier Inc. All rights reserved.

EMP combination chemotherapy and low-dose monotherapy in advanced prostate cancer.

PubMed

Kitamura, Tadaichi; Nishimatsu, Hiroaki; Hamamoto, Toshiaki; Tomita, Kyoichi; Takeuchi, Takumi; Ohta, Nobutaka

2002-02-01

Many chemotherapeutic regimens combined with estramustine phosphate (EMP) have been elaborated for the treatment of hormone-refractory prostate cancer over 30 years. However, older EMP-based combination chemotherapies with vinblastine, vinorelbine, doxorubicin or cyclophosphamide showed relatively low PSA response rate (25-58%) accompanied with high toxicities. On the other hand, newly developed EMP-based combination regimens with etoposide, pacitaxel, carboplatin or docetaxel demonstrated promising PSA response rate (43-77%) with moderate to severe toxicity in the rate of thromboembolic event (5-18%) and of neutropenia (9-41%). Treatment-related death was less in the latter combination group (5/615, 0.8%) than that in the former group (3/234, 1.3%). Of note, in the docetaxel combination with EMP, PSA response rate is as high as 77% with high rate (41%) of neutropenia but no treatment-related death was observed. Docetaxel combination with EMP seems to be the best regimen, though not completely justified by randomized trials, to be selected in the modern era, which will be followed by paclitaxel, carboplatin and EMP combination with PSA response rate of 71%. In addition, an interim report in 83 patients was presented. They were not consecutively enrolled but were treated on low-dose EMP monotherapy for previously untreated advanced prostate cancer in Department of Urology of Tokyo University and our 21 affiliated hospitals. Overall PSA response rate was as high as 93.4% out of 76 assessable patients. However, overall toxicity rate was abnormally high (39.5%) with drug discontinuation rate of 32.1%. The reason of low drug compliance may be attributed to gastrointestinal symptoms. To overcome the low drug compliance, appropriate patients for EMP administration should be selected by using gene analysis on the basis of sophisticated tailor-made medicine.

Effects of High Intensity Training and High Volume Training on Endothelial Microparticles and Angiogenic Growth Factors

PubMed Central

Achtzehn, Silvia; Schmitz, Theresa; Bloch, Wilhelm; Mester, Joachim; Werner, Nikos

2014-01-01

Aims Endothelial microparticles (EMP) are complex vesicular structures shed from activated or apoptotic endothelial cells. As endurance exercise affects the endothelium, the objective of the study was to examine levels of EMP and angiogenic growth factors following different endurance exercise protocols. Methods 12 subjects performed 3 different endurance exercise protocols: 1. High volume training (HVT; 130 min at 55% peak power output (PPO); 2. 4×4 min at 95% PPO; 3. 4×30 sec all-out. EMPs were quantified using flow cytometry after staining platelet-poor-plasma. Events positive for Annexin-V and CD31, and negative for CD42b, were classified as EMPs. Vascular endothelial growth factor (VEGF), migratory inhibiting factor (MIF) and hepatocyte growth factor (HGF) were determined by ELISA technique. For all these measurements venous blood samples were taken pre, 0′, 30′, 60′ and 180′ after each intervention. Furthermore, in vitro experiments were performed to explore the effect of collected sera on target endothelial functions and MP uptake capacities. Results VEGF and HGF significantly increased after HIT interventions. All three interventions caused a significant decrease in EMP levels post exercise compared to pre values. The sera taken after exercise increased the uptake of EMP in target endothelial cells compared to sera taken under resting conditions, which was shown to be phosphatidylserin-dependent. Increased EMP uptake was associated with an improved protection of target cells against apoptosis. Sera taken prior and after exercise promoted target endothelial cell migration, which was abrogated after inhibition of VEGF. Conclusion Physical exercise leads to decreased EMP levels and promotes a phosphatidylserin-dependent uptake of EMP into target endothelial cells, which is associated with a protection of target cells against apoptosis. PMID:24770423

Neoadjuvant luteinizing-hormone-releasing hormone agonist plus low-dose estramustine phosphate improves prostate-specific antigen-free survival in high-risk prostate cancer patients: a propensity score-matched analysis.

PubMed

Koie, Takuya; Mitsuzuka, Koji; Yoneyama, Takahiro; Narita, Shintaro; Kawamura, Sadafumi; Kaiho, Yasuhiro; Tsuchiya, Norihiko; Tochigi, Tatsuo; Habuchi, Tomonori; Arai, Yoichi; Ohyama, Chikara; Yoneyama, Tohru; Tobisawa, Yuki

2015-10-01

The optimal treatment for high-risk prostate cancer (Pca) remains to be established. We previously reported favorable biochemical recurrence-free survival (BRFS) in high-risk Pca patients treated with a neoadjuvant therapy comprising a luteinizing-hormone-releasing hormone (LHRH) agonist plus low dose estramustine phosphate (EMP) (LHRH+EMP) followed by radical prostatectomy (RP). In the present study, we used a retrospective design via propensity score matching to elucidate the clinical benefit of neoadjuvant LHRH+EMP for high-risk Pca. The Michinoku Urological Cancer Study Group database contained data for 1,268 consecutive Pca patients treated with RP alone at 4 institutions between April 2000 and March 2011 (RP alone group). In the RP alone group, we identified 386 high-risk Pca patients. The neoadjuvant LHRH+EMP group included 274 patients with high-risk Pca treated between September 2005 and November 2013 at Hirosaki University. Neoadjuvant LHRH+EMP therapy included LHRH and EMP administration at a dose of 280 mg/day for 6 months before RP. The outcome measures were overall survival (OS) and BRFS. The propensity score-matched analysis indicated 210 matched pairs from both groups. The 5-year BRFS rates were 90.4 and 65.8 % for the neoadjuvant LHRH+EMP and RP alone groups, respectively (P < 0.0001). The 5-year OS rates were 100 and 96.1 % for the neoadjuvant LHRH+EMP and RP alone groups, respectively (P = 0.110). Although the present study was not randomized, neoadjuvant LHRH+EMP therapy followed by RP appeared to reduce the risk of biochemical recurrence. A prospective randomized study is warranted to determine the clinical implications of the neoadjuvant therapy described here.

Catabolic effects of endothelial cell-derived microparticles on disc cells: Implications in intervertebral disc neovascularization and degeneration.

PubMed

Pohl, Pedro H I; Lozito, Thomas P; Cuperman, Thais; Yurube, Takashi; Moon, Hong J; Ngo, Kevin; Tuan, Rocky S; St Croix, Claudette; Sowa, Gwendolyn A; Rodrigues, Luciano M R; Kang, James D; Vo, Nam V

2016-08-01

Neovascularization of intervertebral discs, a phenomenon considered pathological since normal discs are primarily avascular structures, occurs most frequently in annulus fibrosus (AF) of degenerated discs. Endothelial cells (ECs) are involved in this process, but the mechanism of the interaction between AF and endothelial cells is unclear. In this study, we evaluated the effects on matrix catabolic activity of AF cells by the extracellular endothelial microparticles (EMPs) and soluble protein factors (SUP fraction) produced from ECs. Passage 1 human AF cells grown in monolayer cultures were treated for 72 h with 250 µg of EMPs or SUP fraction isolated from culture of the microvascular endothelial cell line, HEMC-I. Live-cell imaging revealed uptake of EMPs by AF cells. RT-PCR analysis demonstrated increased mRNA expression of MMP-1 (50.3-fold), MMP-3 (4.5-fold) and MMP-13 (5.5-fold) in AF cell cultures treated with EMPs compared to untreated control. Western analysis also demonstrated increased MMP protein expression in EMP-treated AF cells. AF cells treated with the SUP fraction also exhibited a dramatic increase in MMP mRNA and protein expression. Increased MMP expression is primarily due to EMP or SUP stimulation of AF cells since EMPs or SUP fraction alone contained negligible amount of MMPs. Interestingly, MMP activity was elevated in AF cell cultures treated with EMPs but not with SUP. This study revealed enhanced matrix catabolism as a molecular consequence of action of ECs on AF cells via EMPs, which might be expected during neo-angiogenesis of degenerating disc. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1466-1474, 2016. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Simulation of coastal floodings during a typhoon event with the consideration of future sea-level rises.

NASA Astrophysics Data System (ADS)

Shu-Huei, Jhang; Chih-Chung, Wen; Dong-Jiing, Doong; Cheng-Han, Tsai

2017-04-01

Taiwan is an Island in the western Pacific Ocean and experienced more than 3 typhoons in a year. Typhoons bring intense rainfall, high waves, and storm surges, which often resulted in coastal flooding. The flooding can be aggravated by the sea level rise due to the global warming, which may subject Taiwan's coastal areas to more serious damage in the future than present. The objectives of this study are to investigate the flooding caused by typhoons in the Annan District, Tainan, a city on the southwest coast of Taiwan by numerical simulations, considering the effects of sea-level rises according to the level suggested by the 5th Assessment Report of IPCC (Intergovernmental Panel on Climate Change) for 2050 and 2100, respectively. The simulations were carried out by using MIKE21 HD (a hydrodynamic model) and MIKE21 SW (a spectral wave model). In our simulation, we used an intense typhoon, named Soudelor, as our base typhoon, which made its landfall on the east coast of Taiwan in the summer of 2015, traveled through the width of the island, and exited the island to the north of Tainan. The reasons we pick this typhoon are that it passed near our objective area, wind field data for this typhoon are available, and we have well documented coastal wave and water level measurements during the passage of Typhoon Soudelor. We firstly used ECMWF (European Centre for Medium-Range Weather Forecasts) wind field data to reconstruct typhoon waves and storm surges for this typhoon by using coupled MIKE21 SW and MIKE21 HD in a regional model. The resultant simulated wave height and sea-level height matched satisfactorily with the measured data. The wave height and storm surge calculated by the regional model provided the boundary conditions for our fine-grid domain. Then different sea-level rises suggested by the IPCC were incorporated into the fine-grid model. Since river discharge due to intense rainfall has also to be considered for coastal flooding, our fine-grid models encompass the estuary of River Yanshui, and measured upstream river discharges were used to simulate the interactions among tide, current, and wave near the estuary of Yanshui River. Our preliminary results showed that with only the effect of rainwater discharge, the maximum surface level of the river during the storm near the estuary was 1.4 m, which is not higher than the river embankments. With the storm surge, the river level at the same location was 2.2 m. With the storm surge and sea-level rise, the maximum river levels near the estuary were 3.6 m and 3.9 m for 2050 and 2100 scenarios, respective. These levels were higher than the embankment height of 3 m. This showed that due to higher sea-level, the area near the estuary will be flooded.

The role of protein kinase C in the opening of blood-brain barrier induced by electromagnetic pulse.

PubMed

Qiu, Lian-Bo; Ding, Gui-Rong; Li, Kang-Chu; Wang, Xiao-Wu; Zhou, Yan; Zhou, Yong-Chun; Li, Yu-Rong; Guo, Guo-Zhen

2010-06-29

The aim of this study was to determine the role of protein kinase C signaling in electromagnetic pulse (EMP)-induced blood-brain barrier (BBB) permeability change in rats. The protein level of total PKC and two PKC isoforms (PKC-alpha, and PKC-beta II) were determined in brain cerebral cortex microvessels by Western blot after exposing rats to EMP at 200kV/m for 200 pulses with 1Hz repetition rate. It was found that the protein level of PKC and PKC-betaII (but not PKC-alpha) in cerebral cortex microvessels increased significantly at 0.5h and 1h after EMP exposure compared with sham-exposed animals and then recovered at 3h. A specific PKC antagonist (H7) almost blocked EMP-induced BBB permeability change. EMP-induced BBB tight junction protein ZO-1 translocation was also inhibited. Our data indicated that PKC signaling was involved in EMP-induced BBB permeability change and ZO-1 translocation in rat.

Nucleosynthesis in Hot Bubbles of SNe-Origin of EMP Stars: HNe or SNe ?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Izutani, Natsuko; Umeda, Hideyuki; Yoshida, Takashi

2010-08-12

The observational trends of extremely metal-poor (EMP) stars reflect SN nucleosynthesis of Population III, or almost metal-free stars. The observation of EMP stars can be reproduced by HNe, not by normal SNe. However, if the innermost neutron-rich or proton-rich matter is ejected, the abundance patterns of ejected matter are changed, and there is a possibility that normal SNe can also reproduce the observations of EMP stars. In this paper, we calculate nucleosynthesis with various Y{sub e} and entropy taking into account neutrino processes. We investigate whether normal SNe with this innermost matter can reproduce the observations of EMP stars. Wemore » find that neutron-rich (Y{sub e} = 0.45-0.50) and proton-rich (Y{sub e} = 0.51-0.55) matters can improve Zn and Co, but tend to overproduce other Fe-peak elements. On the other hand, HNe can naturally reproduce the observations of EMP stars.« less

APT Blanket System Loss-of-Coolant Accident (LOCA) Based on Initial Conceptual Design - Case 4: External Pressurizer Surge Line Break Near Inlet Header

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hamm, L.L.

1998-10-07

This report is one of a series of reports documenting accident scenario simulations for the Accelerator Production of Tritium (APT) blanket heat removal systems. The simulations were performed in support of the Preliminary Safety Analysis Report (PSAR) for the APT.

Assessment of the Great Lakes Marine Renewable Energy Resources: Characterizing Lake Erie Surge, Seiche and Waves

NASA Astrophysics Data System (ADS)

Farhadzadeh, A.; Hashemi, M. R.

2016-02-01

Lake Erie, the fourth largest in surface area, smallest in volume and shallowest among the Great Lakes is approximately 400 km long and 90 km wide. Short term lake level variations are due to storm surge generated by high winds and moving pressure systems over the lake mainly in the southwest-northeast direction, along the lakes longitudinal axis. The historical wave data from three active offshore buoys shows that significant wave height can exceed 5 m in the eastern and central basins. The long-term lake level data show that storm surge can reach up to 3 m in eastern Lake Erie. Owing its shallow depth, Lake Erie frequently experiences seiching motions, the low frequency oscillations that are initiated by storm surge. The seiches whose first mode of oscillations has a period of nearly 14.2 hours can last from several hours to days. In this study, the Lake Erie potential for power generation, primarily using storm surge and seiche and also waves are assessed. Given the cyclic lake level variations due to storm-induced seiching, a concept similar to that of tidal range development is utilized to assess the potential of storm surge and seiche energy harvesting mechanisms for power generation. In addition, wave energy resources of the Lake is characterized -. To achieve these objectives, the following steps are taken : (1) Frequency of occurrence for extreme storm surge and wave events is determined using extreme value analysis such as Peak-Over-Threshold method for the long-term water level and wave data; (2) Spatial and temporal variations of wave height, storm surge and seiche are characterized. The characterization is carried out using the wave and storm surge outputs from numerical simulation of a number of historical extreme events. The coupled ADCIRC and SWAN model is utilized for the modeling; (3) Assessment of the potentials for marine renewable power generation in Lake Erie is made. The approach can be extended to the other lakes in the Great Lakes region.

Down-regulation of EMP1 is associated with epithelial hyperplasia and metaplasia in nasal polyps.

PubMed

Yu, Xue Min; Li, Chun Wei; Li, Ying Ying; Liu, Jing; Lin, Zhi Bin; Li, Tian Ying; Zhao, Li; Pan, Xin Liang; Shi, Li; Wang, De Yun

2013-11-01

The aim of this study was to assess protein and mRNA expression of epithelial membrane protein 1 (EMP1) in the nasal mucosa of patients with nasal polyps (NP), and to determine what changes occur in response to glucocorticosteroid (GC) treatment. NP tissue was obtained from 55 patients, 18 of whom were treated with nasal GCs (i.e. these 18 patients had NP biopsies taken before and after treatment). Biopsies of inferior turbinate mucosa from 30 healthy subjects were used as controls. Quantitative PCR and immunohistochemistry were performed to determine the expression levels of EMP1. EMP1 mRNA expression was significantly lower (2.77-fold) in tissues from NP patients before GC treatment when compared to controls, but was increased in these patients after GC treatment. EMP1 staining in nasal epithelium co-localized with both basal (p63(+)) and differentiated (CK18(+)) epithelial cells. Their immunoreactivity was significantly greater in controls than NP patients. EMP1 mRNA levels were lower in the epithelium with severe hyperplasia (1.79-fold) or with metaplasia (1.85-fold) as compared to those with mild to moderate hyperplasia or non-metaplastic epithelium, respectively. Positive correlations between EMP1 and other epithelial cell-related gene (e.g. JUN, PTGS2, AREG etc.) mRNAs were observed. EMP1 could be a biomarker for aberrant epithelial remodelling and metaplasia in chronic inflammatory upper airway mucosa (e.g. NP). © 2013 John Wiley & Sons Ltd.

[Effects of electromagnetic pulses on apoptosis and TGF-β3 expression of mouse testis tissue].

PubMed

Luo, Yaning; Ding, Guirong; Chen, Yongbin; Xu, Shenglong; Wang, Xiaowu

2014-04-01

To investigate the effects of electromagnetic pulses (EMP) on the apoptosis and transforming growth factor beta 3 (TGF-β3) expression of mouse testis tissue. Thirty-two male BALB/c mice were randomly and equally divided into one control group and three EMP treated groups, which were whole-body exposed to EMP at 200 kV/m with 100, 200, and 400 pulses, respectively. The control group received no treatment. The pathological changes and cell apoptosis in testis tissue were analyzed by TUNEL assay. The mRNA expression of TGF-β3 in testis tissue was determined by RT-PCR, and the protein expression of TGF-β3 was determined by immunohistochemistry and Western blot. No obvious pathological changes were found in testis tissue after EMP exposure at 200 kV/m with 100 and 200 pulses. However, after EMP exposure with 400 pulses, degeneration and shedding of testis tissue, accompanied by significant increase in apoptosis rate (P < 0.05), was observed. The RT-PCR, immunohistochemistry, and Western blot showed that the expression of TGF-β3 mRNA and protein increased significantly after EMP exposure with 400 pulses as compared with that of the control group (P < 0.05). EMP exposure at 200 kV/m with 400 pulses increases the incidence of apoptosis and expression of TGF-β3 in mouse testis tissue, which is potentially one of the mechanisms by which EMP increases blood-testis barrier permeability in mice.

Clinical analysis of 40 multiple myeloma patients with extramedullary plasmacytoma of the head

PubMed Central

Sun, Wan-jun; Zhang, Jia-jia; An, Na; Shen, Men; Huang, Zhong-xia

2016-01-01

Objectives To investigate the clinical characteristics, survival and prognosis of patients with multiple myeloma (MM) and head extramedullary plasmacytoma (EMP). Methods Forty MM patients were enrolled in the study (18 men, 22 women; median age, 55 years). Results Median overall survival (OS) and progression-free survival (PFS) were 24 (5–78) months and 17 (2–36) months, respectively. The 2-, 3- and 5-year OS rates were 51%, 20% and 7%, respectively. The 2-year PFS was 15%. Median OS and PFS in patients administered velcade were 26 (18–50) and 22.5 (5–78) months, compared with 20 (10–30) and 13.5 (2–36) months in patients without velcade, respectively. Median OS was 23.5 (5–50) months in patients with EMP at MM diagnosis (n = 25) and 36 (22–78) months in patients with head EMP diagnosed during the disease course (n = 15). Sixteen MM patients had EMP invasion of the head only and 24 had invasion at multiple sites. Median OS was 25 (22–78) months in patients with EMP of the head only and 22 (5–78) months in patients with EMP invasion at multiple sites. Conclusion MM patients with head EMP show a more aggressive disease course and shorter OS and PFS. The prognosis of these patients is poor, especially in patients with head EMP at MM diagnosis, though combined chemotherapy and radiotherapy may prolong survival. PMID:28322099

Metal-poor star formation triggered by the feedback effects from Pop III stars

NASA Astrophysics Data System (ADS)

Chiaki, Gen; Susa, Hajime; Hirano, Shingo

2018-04-01

Metal enrichment by first-generation (Pop III) stars is the very first step of the matter cycle in structure formation and it is followed by the formation of extremely metal-poor (EMP) stars. To investigate the enrichment process by Pop III stars, we carry out a series of numerical simulations including the feedback effects of photoionization and supernovae (SNe) of Pop III stars with a range of masses of minihaloes (MHs), Mhalo, and Pop III stars, MPopIII. We find that the metal-rich ejecta reach neighbouring haloes and external enrichment (EE) occurs when the H II region expands before the SN explosion. The neighbouring haloes are only superficially enriched, and the metallicity of the clouds is [Fe/H] < -5. Otherwise, the SN ejecta fall back and recollapse to form an enriched cloud, i.e. an internal-enrichment (IE) process takes place. In the case where a Pop III star explodes as a core-collapse SN (CCSN), the MH undergoes IE, and the metallicity in the recollapsing region is -5 ≲ [Fe/H] ≲ -3 in most cases. We conclude that IE from a single CCSN can explain the formation of EMP stars. For pair-instability SNe (PISNe), EE takes place for all relevant mass ranges of MHs, consistent with the lack of observational signs of PISNe among EMP stars.

Nonlinear control of rotating stall and surge with axisymmetric bleed and air injection on axial flow compressors

NASA Astrophysics Data System (ADS)

Yeung, Chung-Hei (Simon)

The study of compressor instabilities in gas turbine engines has received much attention in recent years. In particular, rotating stall and surge are major causes of problems ranging from component stress and lifespan reduction to engine explosion. In this thesis, modeling and control of rotating stall and surge using bleed valve and air injection is studied and validated on a low speed, single stage, axial compressor at Caltech. Bleed valve control of stall is achieved only when the compressor characteristic is actuated, due to the fast growth rate of the stall cell compared to the rate limit of the valve. Furthermore, experimental results show that the actuator rate requirement for stall control is reduced by a factor of fourteen via compressor characteristic actuation. Analytical expressions based on low order models (2--3 states) and a high fidelity simulation (37 states) tool are developed to estimate the minimum rate requirement of a bleed valve for control of stall. A comparison of the tools to experiments show a good qualitative agreement, with increasing quantitative accuracy as the complexity of the underlying model increases. Air injection control of stall and surge is also investigated. Simultaneous control of stall and surge is achieved using axisymmetric air injection. Three cases with different injector back pressure are studied. Surge control via binary air injection is achieved in all three cases. Simultaneous stall and surge control is achieved for two of the cases, but is not achieved for the lowest authority case. This is consistent with previous results for control of stall with axisymmetric air injection without a plenum attached. Non-axisymmetric air injection control of stall and surge is also studied. Three existing control algorithms found in literature are modeled and analyzed. A three-state model is obtained for each algorithm. For two cases, conditions for linear stability and bifurcation criticality on control of rotating stall are derived and expressed in terms of implementation-oriented variables such as number of injectors. For the third case, bifurcation criticality conditions are not obtained due to complexity, though linear stability property is derived. A theoretical comparison between the three algorithms is made, via the use of low-order models, to investigate pros and cons of the algorithms in the context of operability. The effects of static distortion on the compressor facility at Caltech is characterized experimentally. Results consistent with literature are obtained. Simulations via a high fidelity model (34 states) are also performed and show good qualitative as well as quantitative agreement to experiments. A non-axisymmetric pulsed air injection controller for stall is shown to be robust to static distortion.

Modeling the Effects of Storm Surge from Hurricane Jeanne on Saltwater Intrusion into the Surficial Aquifer, East-Central Florida (USA)

NASA Astrophysics Data System (ADS)

Xiao, H.; Wang, D.; Hagen, S. C.; Medeiros, S. C.; Hall, C. R.

2017-12-01

Saltwater intrusion (SWI) that has been widely recognized as a detrimental issue causing the deterioration of coastal aquifer water quality and degradation of coastal ecosystems. While it is widely recognized that SWI is exacerbated worldwide due to global sea-level rise, we show that increased SWI from tropical cyclones under climate change is also a concern. In the Cape Canaveral Barrier Island Complex (CCBIC) located in east-central Florida, the salinity level of the surficial aquifer is of great importance to maintain a bio-diverse ecosystem and to support the survival of various vegetation species. Climate change induced SWI into the surficial aquifer can lead to reduction of freshwater storage and alteration of the distribution and productivity of vegetation communities. In this study, a three-dimensional variable-density SEAWAT model is developed and calibrated to investigate the spatial and temporal variation of salinity level in the surficial aquifer of CCBIC. We link the SEAWAT model to surge model data to examine the effects of storm surge from Hurricane Jeanne. Simulation results indicate that the surficial aquifer salinity level increases significantly right after the occurrence of storm surge because of high aquifer permeability and rapid infiltration and diffusion of the overtopping saltwater, while the surficial aquifer salinity level begins to decrease after the fresh groundwater recharge from the storm's rainfall. The tropical storm precipitation generates an effective hydraulic barrier further impeding SWI and providing seaward freshwater discharge for saltwater dilution and flushing. To counteract the catastrophic effects of storm surge, this natural remediation process may take at least 15-20 years or even several decades. These simulation results contribute to ongoing research focusing on forecasting regional vegetation community responses to climate change, and are expected to provide a useful reference for climate change adaptation planning and decision-making in the CCBIC and other low-lying coastal alluvial plains and barrier island systems.

Ocean modelling and Early-Warning System for the Gulf of Thailand

NASA Astrophysics Data System (ADS)

de Lima Rego, Joao; Yan, Kun; Sisomphon, Piyamarn; Thanathanphon, Watin; Twigt, Daniel; Irazoqui Apecechea, Maialen

2017-04-01

Storm surges associated with severe tropical cyclones are among the most hazardous and damaging natural disasters to coastal areas. The Gulf of Thailand (GoT) has been periodically affected by typhoon induced storm surges in the past (e.g. storm Harriet in 1962, storm Gay in 1989 and storm Linda in 1997). Due to increased touristic / economic development and increased population density in the coastal zone, the combined effect and risk of high water level and increased rainfall / river discharge has dramatically increased and are expected to increase in future due to climate change effects. This presentation describes the development and implementation of the first real-time operational storm surge, wave and wave setup forecasting system in the GoT, a joint applied research initiative by Deltares in The Netherlands and the Hydro and Agro Informatics Institute (HAII) in Thailand. The modelling part includes a new hydrodynamic model to simulate tides and storm surges and two wave models (regional and local). The hydrodynamic model is based on Delft3D Flexible Mesh, capable of simulating water levels and detailed flows. The regional and the recently-developed local wave model are based on the SWAN model, a third-generation wave model. The operational platform is based on Delft-FEWS software, which coordinates all the data inputs, the modelling tasks and the automatic forecast exports including overland inundation in the upper Gulf of Thailand. The main objective of the Gulf of Thailand EWS is to provide daily accurate storm surge, wave and wave setup estimates automatically with various data exports possibilities to support this task. It adds a coastal component to HAII's existing practice of providing daily reports on fluvial flood forecasts, used for decision-support in issuing flood warnings for inland water systems in Thailand. Every day, three-day coastal forecasts are now produced based on the latest regional meteorological predictions. Examples are given to illustrate the system's development and main features, with a focus on decision-support products.

Impact of Variable SST on Simulated Warm Season Precipitation

NASA Astrophysics Data System (ADS)

Saleeby, S. M.; Cotton, W. R.

2007-05-01

The Colorado State University - Regional Atmospheric Modeling System (CSU-RAMS) is being used to examine the variability in monsoon-related warm season precipitation over Mexico and the United States due to variability in SST. Given recent improvements and increased resolution in satellite derived SSTs it is pertinent to examine the sensitivity of the RAMS model to the variety of SST data sources that are available. In particular, we are examining this dependence across continental scales over the full warm season, as well as across the regional scale centered around the Gulf of California on time scales of individual surge events. In this study we performed an ensemble of simulations that include the 2002, 2003, and 2004 warm seasons with use of the Climatology, Reynold's, AVHRR, and MODIS SSTs. From the seasonal 90-day simulations with 30km grid spacing, it was found that variations in surface latent heat flux are directly linked to differences in SST. Regions with cooler (warmer) SST have decreased (increased) moisture flux from the ocean which is in proportion to the magnitude of the SST difference. Over the eastern Pacific, differences in low-level horizontal moisture flux show a general trend toward reduced fluxes over cooler waters and very little inland impact. Over the Gulf of Mexico, however, there is substantial variability for each dataset comparison, despite having only limited variability among the SST data. Causes of this unexpected variability are not straight-forward. Precipitation impacts are greatest near the southern coast of Mexico and along the Sierra Madres. Precipitation variability over the CONUS is rather chaotic and is limited to areas impacted by the Gulf of Mexico or monsoon convection. Another unexpected outcome is the lack of variability in areas near the northern Gulf of California where SST and latent heat flux variability is a maximum. From the 7-day surge period simulations at 7km grid spacing, we found that SST differences on the higher resolution nested grid reveal fine scale variability that is otherwise smoothed out or unapparent on the coarser grid. Unlike the coarse grid, the latent heat flux, temperature, and moisture transport differences on the fine grid reveal an inland impact. This is likely due to fine scale variability in onshore moisture transport and sea- breeze circulations which may alter monsoonal convection and precipitation. However, only the largest SST differences (spatially and in magnitude) tend to invoke large, coherent responses in moisture flux. The SST variability at high resolution produces relatively large differences in precipitation that are focused along the slopes of the SMO, with a tendency toward greater variability along the western slope adjacent to the coast. The precipitation differences are of fine resolution, with variability of +/- 30 mm (over 5 days) along the length of the SMO. Variability on the fine grid also invokes precipitation changes over AZ/NM that are not resolved on the coarse grid. Vertical cross-sections examined along the GoC during the surge episode revealed variations in the moisture and temperature structure of the surge. The cooler SSTs in the climatological dataset produced the greatest variability compared to the other datasets. The surge produced from climatology SSTs was nearly 5g/kg drier and up to 4°C cooler compared to surges influenced by the SST datasets. The overall northward propagation of the surge appeared unaffected by the SSTs.

Impact of EMP on Air Operations

DTIC Science & Technology

pulse while in flight. With a requirement to be compliant with this standard for all critical weapon systems, the C -17 remains untested. The significant...EMP event that would threaten an inflight C -17, there was not enough evidence to force a change to the current EMP testing and hardening schedule.

Effects of electromagnetic pulse on bone metabolism of mice in vivo.

PubMed

Li, Kang-Chu; Ma, Shi-Rong; Ding, Gui-Rong; Guo, Yao; Guo, Guo-Zhen

2009-12-01

To study the effects of electromagnetic pulse (EMP) on bone metabolism of mice in vivo. Twenty-four male BALB/c mice were divided into a control group and 2 experimental groups (n=8). The whole-body of mice in experimental groups were exposed to 50 kV/m and 400kV/m EMP, 400 pulses daily for 7 consecutive days at 2 seconds intervals. Alkaline phosphotase (ALP) activity, serum calcium concentration and osteocalcin level and trabecular bone volume (BV/TV, %) were measured immediately after EMP exposure by biochemical, ELISA and morphological methods. The ALP activity, serum calcium concentration and osteocalcin level and BV/TV in experimental groups remained unchanged after EMP exposure. Conclusion Under our experimental conditions, EMP exposure cannot affect bone metabolism of mice in vivo.

Iron-regulated biofilm formation in Staphylococcus aureus Newman requires ica and the secreted protein Emp.

PubMed

Johnson, Miranda; Cockayne, Alan; Morrissey, Julie A

2008-04-01

Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface and have previously been implicated in a number of aspects of S. aureus pathogenesis. We showed here that the transcription of these important virulence factors is induced by growth in low-iron medium, reflective of the in vivo environment. Our results show that iron regulation of Eap and Emp is Fur independent. However, Fur is required for full induction of eap and emp expression in low-iron conditions. In this study, we demonstrated that in addition to Fur, low-iron-induced biofilm formation requires Sae, Agr, and SarA. In iron-restricted growth conditions, Sae and Agr are essential for Emp and Eap expression and hence for biofilm formation, whereas SarA appears to have a less-significant role. We also showed that expression of the ica operon is required for biofilm formation in iron-restricted growth conditions. We demonstrated that in fact, ica is required for the expression of the important multifunctional virulence determinants eap and emp.

The Staphylococcus aureus extracellular matrix protein (Emp) has a fibrous structure and binds to different extracellular matrices.

PubMed

Geraci, Jennifer; Neubauer, Svetlana; Pöllath, Christine; Hansen, Uwe; Rizzo, Fabio; Krafft, Christoph; Westermann, Martin; Hussain, Muzaffar; Peters, Georg; Pletz, Mathias W; Löffler, Bettina; Makarewicz, Oliwia; Tuchscherr, Lorena

2017-10-20

The extracellular matrix protein Emp of Staphylococcus aureus is a secreted adhesin that mediates interactions between the bacterial surface and extracellular host structures. However, its structure and role in staphylococcal pathogenesis remain unknown. Using multidisciplinary approaches, including circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopy, transmission electron (TEM) and immunogold transmission electron microscopy, functional ELISA assays and in silico techniques, we characterized the Emp protein. We demonstrated that Emp and its truncated forms bind to suprastructures in human skin, cartilage or bone, among which binding activity seems to be higher for skin compounds. The binding domain is located in the C-terminal part of the protein. CD spectroscopy revealed high contents of β-sheets (39.58%) and natively disordered structures (41.2%), and TEM suggested a fibrous structure consisting of Emp polymers. The N-terminus seems to be essential for polymerization. Due to the uncommonly high histidine content, we suggest that Emp represents a novel type of histidine-rich protein sharing structural similarities to leucine-rich repeats proteins as predicted by the I-TASSER algorithm. These new findings suggest a role of Emp in infections of deeper tissue and open new possibilities for the development of novel therapeutic strategies.

Structure and biological activities of eumenine mastoparan-AF (EMP-AF), a new mast cell degranulating peptide in the venom of the solitary wasp (Anterhynchium flavomarginatum micado).

PubMed

Konno, K; Hisada, M; Naoki, H; Itagaki, Y; Kawai, N; Miwa, A; Yasuhara, T; Morimoto, Y; Nakata, Y

2000-11-01

A new mast cell degranulating peptide, eumenine mastoparan-AF (EMP-AF), was isolated from the venom of the solitary wasp Anterhynchium flavomarginatum micado, the most common eumenine wasp found in Japan. The structure was analyzed by FAB-MS/MS together with Edman degradation, which was corroborated by solid-phase synthesis. The sequence of EMP-AF, Ile-Asn-Leu-Leu-Lys-Ile-Ala-Lys-Gly-Ile-Ile-Lys-Ser-Leu-NH(2), was similar to that of mastoparan, a mast cell degranulating peptide from a hornet venom; tetradecapeptide with C-terminus amidated and rich in hydrophobic and basic amino acids. In fact, EMP-AF exhibited similar activity to mastoparan in stimulating degranulation from rat peritoneal mast cells and RBL-2H3 cells. It also showed significant hemolytic activity in human erythrocytes. Therefore, this is the first example that a mast cell degranulating peptide is found in the solitary wasp venom. Besides the degranulation and hemolytic activity, EMP-AF also affects on neuromuscular transmission in the lobster walking leg preparation. Three analogs EMP-AF-1 approximately 3 were snythesized and biologically tested together with EMP-AF, resulting in the importance of the C-terminal amide structure for biological activities.

Restrain of bone growth by estrogen-mimetic peptide-1 (EMP-1): a micro-computed tomographic study.

PubMed

Kasher, Roni; Bajayo, Alon; Gabet, Yankel; Nevo, Nava; Fridkin, Mati; Katchalski-Katzir, Ephraim; Kohen, Fortune; Bab, Itai

2009-06-01

Estrogen has a key role in the regulation of skeletal growth and maintenance of bone mass. Recently, we developed peptides having estrogen-like activity as potential estrogen-based new drugs. The aim of the present study was to evaluate the influence of long-term administration of the most efficacious of these peptides, the hexapeptide EMP-1 (VSWFFE), on bone mass and development. EMP-1 was injected daily to ovariectomized (OVX) and intact young, sexually mature female mice for 10 weeks. Whole femora, including the cartilaginous growth plates were analyzed by micro-computed tomography (microCT). We found that peptide EMP-1 restrains bone growth in OVX mice: it inhibited dramatically bone longitudinal growth (40%), and decreased femoral diaphyseal diameter. Peptide EMP-1 had no effect on bone growth in normal mice, and did not influence the OVX-induced bone loss. We then developed a new microCT methodology to evaluate uncalcified and calcified growth plate parameters. In the OVX mice, peptide EMP-1 reduced volume and thickness of the uncalcified growth plate, a possible cause for the inhibition of bone longitudinal growth. Peptide EMP-1 may be used as a lead compound for the development of drugs to treat acromegalic patients.

[Effects of electromagnetic pulse exposure on the morphological change and excretion function of BV-2 cells and possible mechanism].

PubMed

Yang, Long-long; Zhou, Yan; Li, Hai-juan; Guo, Juan; Zhang, Yan-jun; Ding, Gui-rong; Guo, Guo-zhen

2012-03-01

To study the effects of electromagnetic pulse (EMP) exposure on the morphological change and excretion functions of mouse microglia (BV-2) cells and possible mechanism. BV-2 cells were divided into two groups: the group exposed to EMP at 200 kV/m for 200 pulses and sham exposure group. At 1, 6, 12 and 24 hour after exposure the cells and culture supernatant were collected. Cellular morphological change was observed under invert microscope, the levels of TNF-α, IL-1β and IL-10 in culture supernatant were determined by enzyme-linked immunosorbent assay (ELISA), nitric oxide (NO) and reactive oxygen species (ROS) were detected by nitrate reductase method and DCFH-DA probe, respectively. The protein and phosphorylation levels of ERK, JNK and p38 were measured by Western Blot method. After the cells pre-treated with the inhibitor of p38 (SB203580) were exposed to EMP, the levels of NO and ROS in culture supernatant were detected. It was found that the large ameboid shape appeared in some microglia cells exposed to EMP for 1, 6 and 12 h. Moreover, the number of microglia cells with ameboid shape increased significantly at 1 h, 6 h and 12 h after EMP exposure compared with sham group (P < 0.05). The levels of cytokines, such as TNF-α, IL-1β and IL-10, in culture supernatant did not change obviously after EMP exposure. The levels of NO and ROS increased significantly at 1h after EMP exposure, reached the peak at 6 h, began to recover at 12 h and recovered to sham group level at 24 h (P < 0.05). Western blot results showed that the protein and protein phosphorylation levels of ERK and JNK did not change significantly after EMP exposure, however, the protein and protein phosphorylation levels of p38 increased obviously at 1 h and 6 h after EMP exposure, compared with sham group (P < 0.05). In addition, the pretreatment of p38 inhibitor (SB203580) significantly decreased NO and ROS production induced by EMP. EMP exposure may activate microglia cells and promote the production of NO and ROS in mouse microglia cells, and p38 pathway is involved in this process.

Sensitivity of Hurricane Storm Surge to Land Cover and Topography Under Various Sea Level Rise Scenarios Along the Mississippi Coast

NASA Astrophysics Data System (ADS)

Bilskie, M. V.; Hagen, S. C.; Medeiros, S. C.

2013-12-01

Major Gulf hurricanes have a high probability of impacting the northern Gulf of Mexico, especially coastal Mississippi (Resio, 2007). Due to the wide and flat continental shelf, this area provides near-perfect geometry for high water levels under tropical cyclone conditions. Literature suggests with 'very high confidence that global sea level will rise at least 0.2 m and no more than 2.0 m by 2011' (Donoghue, 2011; Parris et al., 2012). Further, it is recognized that the Mississippi barrier islands are highly susceptible to a westward migration and retreating shoreline. With predictions for less frequent, more intense tropical storms, rising sea levels, and a changing landscape, it is important to understand how these changes may affect inundation extent and flooding due to hurricane storm surge. A state-of-the-art SWAN+ADCIRC hydrodynamic model of coastal Mississippi was utilized to simulate Hurricane Katrina with present day sea level conditions. Using present day as a base scenario, past (1960) and future (2050) sea level changes were simulated. In addition to altering the initial sea state, land use land cover (LULC) was modified for 1960 and 2050 based on historic data and future projections. LULC datasets are used to derive surface roughness characteristics, such as Manning's n, and wind reduction factors. The topography along the barrier islands and near the Pascagoula River, MS was also altered to reflect the 1960 landscape. Storm surge sensitivity to topographic change were addressed by comparing model results between two 1960 storm surge simulations; one with current topography and a second with changes to the barrier islands. In addition, model responses to changes in LULC are compared. The results will be used to gain insight into adapting present day storm surge models for future conditions. References Donoghue, J. (2011). Sea level history of the northern Gulf of Mexico coast and sea level rise scenarios for the near future. Climatic Change, 107(1-2), 17-33. doi: 10.1007/s10584-011-0077-x Parris, A., Bromirski, P., Burkett, V., Cayan, D., Culver, M., Hall, J., . . . Weiss, J. (2012). Global Sea Level Rise Scenarios for the United States National Climate Assessment NOAA Tech Memo OAR CPO-1 (pp. 37). Resio, D. T. (2007). White paper on estimating hurricane inundation probabilities (pp. 125). Vicksburg, MS: U.S. Army Engineering Research and Development Center.

WEC-SIM Phase 1 Validation Testing -- Numerical Modeling of Experiments: Preprint

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruehl, Kelley; Michelen, Carlos; Bosma, Bret

2016-08-01

The Wave Energy Converter Simulator (WEC-Sim) is an open-source code jointly developed by Sandia National Laboratories and the National Renewable Energy Laboratory. It is used to model wave energy converters subjected to operational and extreme waves. In order for the WEC-Sim code to be beneficial to the wave energy community, code verification and physical model validation is necessary. This paper describes numerical modeling of the wave tank testing for the 1:33-scale experimental testing of the floating oscillating surge wave energy converter. The comparison between WEC-Sim and the Phase 1 experimental data set serves as code validation. This paper is amore » follow-up to the WEC-Sim paper on experimental testing, and describes the WEC-Sim numerical simulations for the floating oscillating surge wave energy converter.« less

Future hurricane storm surge risk for the U.S. gulf and Florida coasts based on projections of thermodynamic potential intensity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Balaguru, Karthik; Judi, David R.; Leung, L. Ruby

Coastal populations in the global tropics and sub-tropics are vulnerable to the devastating impacts of hurricane storm surge and this risk is only expected to rise under climate change. In this study, we address this issue for the U.S. Gulf and Florida coasts. Using the framework of Potential Intensity, observations and output from coupled climate models, we show that the future large-scale thermodynamic environment may become more favorable for hurricane intensification. Under the RCP 4.5 emissions scenario and for the peak hurricane season months of August–October, we show that the mean intensities of Atlantic hurricanes may increase by 1.8–4.2 %more » and their lifetime maximum intensities may increase by 2.7–5.3 % when comparing the last two decades of the 20th and 21st centuries. We then combine our estimates of hurricane intensity changes with projections of sea-level rise to understand their relative impacts on future storm surge using simulations with the National Weather Service’s SLOSH (Sea, Lake, and Overland Surges from Hurricanes) model for five historical hurricanes that made landfall in the Gulf of Mexico and Florida. Considering uncertainty in hurricane intensity changes and sea-level rise, our results indicate a median increase in storm surge ranging between 25 and 47 %, with changes in hurricane intensity increasing future storm surge by about 10 % relative to the increase that may result from sea level rise alone, with highly non-linear response of population at risk.« less

Numerical modeling of storm surges in the coast of Mozambique: the cases of tropical cyclones Bonita (1996) and Lisette (1997)

NASA Astrophysics Data System (ADS)

Bié, Alberto José; de Camargo, Ricardo; Mavume, Alberto Francisco; Harari, Joseph

2017-11-01

The coast of Mozambique is often affected by storms, particularly tropical cyclones during summer or sometimes midlatitude systems in the southern part. Storm surges combined with high freshwater discharge can drive huge coastal floods, affecting both urban and rural areas. To improve the knowledge about the impact of storm surges in the coast of Mozambique, this study presents the first attempt to model this phenomenon through the implementation of the Princeton Ocean Model (POM) in the Southwestern Indian Ocean domain (SWIO; 2-32°S, 28-85°E) using a regular grid with 1/6° of spatial resolution and 36 sigma levels. The simulation was performed for the period 1979-2010, and the most interesting events of surges were related to tropical cyclones Bonita (1996) and Lisette (1997) that occurred in the Mozambique Channel. The results showed that the model represented well the amplitude and phase of principal lunar and solar tidal constituents, as well as it captured the spatial pattern and magnitudes of SST with slight positive bias in summer and negative bias in winter months. In terms of SSH, the model underestimated the presence of mesoscale eddies, mainly in the Mozambique Channel. Our results also showed that the atmospheric sea level pressure had a significant contribution to storm heights during the landfall of the tropical cyclones Bonita (1996) and Lisette (1997) in the coast of Mozambique contributing with about 20 and 16% of the total surge height for each case, respectively, surpassing the contribution of the tide-surge nonlinear interactions by a factor of 2.

Impact of an emergency medicine pharmacist on antibiotic dosing adjustment.

PubMed

DeWitt, Kyle M; Weiss, Steven J; Rankin, Shannon; Ernst, Amy; Sarangarm, Preeyaporn

2016-06-01

Overall medication-related errors in the emergency department (ED) are 13.5 times more likely to occur in the absence of an emergency medicine pharmacist (EMP). Although the effectiveness of pharmacist-driven renal dosing adjustment has been studied in the intensive care unit, data are lacking in the ED setting. The aim of our study was to evaluate the appropriateness of antibiotic dosing when an EMP is physically present in the ED compared to when absent. This was a retrospective cohort study of patients treated in a level I trauma center with 75 adult and 12 pediatric beds and an annual census of 90000 patients. The study period was from March 1 to September 30, 2014. An EMP was physically present in the ED from 11:00 to 01:30 and absent from 01:31 to 10:59. Male and female patients 18years and older were considered for inclusion if cefazolin, cefepime, ciprofloxacin, piperacillin-tazobactam, or vancomycin was ordered. The primary outcome was the composite rate of correct antibiotic dose and frequency. Statistics included a multivariable logistic regression using age, sex, presence of EMP, and creatinine clearance as independent predictors of correct antibiotic use. A total 210 cases were randomly chosen for evaluation, half during times when EMPs were present and half when they were absent. There were 130 males (62%) with an overall mean age of 54±18years. Overall, 178 (85%) of 210 of the antibiotic orders were appropriate, with 95% appropriate when an EMP was present compared to 74% when an EMP was absent (odds ratio, 6.9; 95% confidence interval, 2.5-18.8). In a logistic regression model, antibiotic appropriateness was independently associated with the presence of the EMP and creatinine clearance. Antibiotics that require renal and/or weight dosing adjustment are 6.5 times more likely to be appropriate in the ED when an EMP is present. Prevalence of antibiotic dosing error is related to both the presence of EMPs and the degree of renal impairment. Copyright © 2016 Elsevier Inc. All rights reserved.

Evaluation of Surface Roughness of Ceramic and Resin Composite Material Used for Conservative Indirect Restorations, after Repolishing by Intraoral Means.

PubMed

Vrochari, Areti D; Petropoulou, Aikaterini; Chronopoulos, Vasilios; Polydorou, Olga; Massey, Ward; Hellwig, Elmar

2017-06-01

To evaluate and compare the mean surface roughness (Ra) of one ceramic and one resin composite material used for indirect restorations, after grinding and repolishing by intraoral means. The materials used were the lithium disilicate glass ceramic IPS e.max Press (EMP) and the indirect resin composite restoration system Gradia (GR). Twelve specimen disks were prepared from each material according to the manufacturer of each material. Five initial measurements of the Ra (Ra 1 ) were made on each specimen as a referral basis, and the specimens were ground with a fine (red) diamond bur. The specimens were repolished using (a) Komet Dialite Polishing Kit for EMP and (b) Enhance Finishing and Polishing System and Prisma Gloss Polishing Paste for GR. Five final Ra (Ra 2 ) measurements were performed on each specimen. All measurements were made using a laser profilometer. Scanning electron microscopy (SEM) was also used to visualize the initial surface morphology and the morphological changes on the specimens' surface after repolishing. A highly significant difference was found between Ra 1EMP and Ra 2EMP (p < 0.001), between Ra 1GR and Ra 2GR (p < 0.001), as well as between Ra 2EMP and Ra 2GR (p < 0.001), when compared in pairs. A highly significant difference (p < 0.001) was also found between ΔRa EMP and ΔRa GR , with ΔRa GR being higher than ΔRa EMP . The Ra GR values were higher than the Ra EMP values at all times. SEM revealed that both EMP and GR repolished surfaces presented with irregularities; however, in GR specimens major voids and craters were present. EMP was found to perform better when polished by intraoral means compared with GR. Both materials exhibited Ra 2 above the critical threshold for increased plaque accumulation and periodontal inflammation. If enamel-to-enamel roughness found in occlusal contact areas is considered as baseline, both materials were clinically acceptable after repolishing. © 2015 by the American College of Prosthodontists.

A Storm Surge and Inundation Model of the Back River Watershed at NASA Langley Research Center

NASA Technical Reports Server (NTRS)

Loftis, Jon Derek; Wang, Harry V.; DeYoung, Russell J.

2013-01-01

This report on a Virginia Institute for Marine Science project demonstrates that the sub-grid modeling technology (now as part of Chesapeake Bay Inundation Prediction System, CIPS) can incorporate high-resolution Lidar measurements provided by NASA Langley Research Center into the sub-grid model framework to resolve detailed topographic features for use as a hydrological transport model for run-off simulations within NASA Langley and Langley Air Force Base. The rainfall over land accumulates in the ditches/channels resolved via the model sub-grid was tested to simulate the run-off induced by heavy precipitation. Possessing both the capabilities for storm surge and run-off simulations, the CIPS model was then applied to simulate real storm events starting with Hurricane Isabel in 2003. It will be shown that the model can generate highly accurate on-land inundation maps as demonstrated by excellent comparison of the Langley tidal gauge time series data (CAPABLE.larc.nasa.gov) and spatial patterns of real storm wrack line measurements with the model results simulated during Hurricanes Isabel (2003), Irene (2011), and a 2009 Nor'easter. With confidence built upon the model's performance, sea level rise scenarios from the ICCP (International Climate Change Partnership) were also included in the model scenario runs to simulate future inundation cases.

Impact of hydrogeological factors on groundwater salinization due to ocean-surge inundation

NASA Astrophysics Data System (ADS)

Yang, Jie; Zhang, Huichen; Yu, Xuan; Graf, Thomas; Michael, Holly A.

2018-01-01

Ocean surges cause seawater inundation of coastal inland areas. Subsequently, seawater infiltrates into coastal aquifers and threatens the fresh groundwater resource. The severity of resulting salinization can be affected by hydrogeological factors including aquifer properties and hydrologic conditions, however, little research has been done to assess these effects. To understand the impacts of hydrogeological factors on groundwater salinization, we numerically simulated an ocean-surge inundation event on a two-dimensional conceptual coastal aquifer using a coupled surface-subsurface approach. We varied model permeability (including anisotropy), inland hydraulic gradient, and recharge rate. Three salinization-assessment indicators were developed, based on flushing time, depth of salt penetration, and a combination of the two, weighted flushing time, with which the impact of hydrogeological factors on groundwater vulnerability to salinization were quantitatively assessed. The vulnerability of coastal aquifers increases with increasing isotropic permeability. Low horizontal permeability (kx) and high vertical permeability (kz) lead to high aquifer vulnerability, and high kx and low kz lead to low aquifer vulnerability. Vulnerability decreases with increasing groundwater hydraulic gradient and increasing recharge rate. Additionally, coastal aquifers with a low recharge rate (R ≤ 300 mm yr-1) may be highly vulnerable to ocean-surge inundation. This study shows how the newly introduced indicators can be used to quantitatively assess coastal aquifer vulnerability. The results are important for global vulnerability assessment of coastal aquifers to ocean-surge inundation.

Uncertainties in extreme surge level estimates from observational records.

PubMed

van den Brink, H W; Können, G P; Opsteegh, J D

2005-06-15

Ensemble simulations with a total length of 7540 years are generated with a climate model, and coupled to a simple surge model to transform the wind field over the North Sea to the skew surge level at Delfzijl, The Netherlands. The 65 constructed surge records, each with a record length of 116 years, are analysed with the generalized extreme value (GEV) and the generalized Pareto distribution (GPD) to study both the model and sample uncertainty in surge level estimates with a return period of 104 years, as derived from 116-year records. The optimal choice of the threshold, needed for an unbiased GPD estimate from peak over threshold (POT) values, cannot be determined objectively from a 100-year dataset. This fact, in combination with the sensitivity of the GPD estimate to the threshold, and its tendency towards too low estimates, leaves the application of the GEV distribution to storm-season maxima as the best approach. If the GPD analysis is applied, then the exceedance rate, lambda, chosen should not be larger than 4. The climate model hints at the existence of a second population of very intense storms. As the existence of such a second population can never be excluded from a 100-year record, the estimated 104-year wind-speed from such records has always to be interpreted as a lower limit.

Stall/surge dynamics of a multi-stage air compressor in response to a load transient of a hybrid solid oxide fuel cell-gas turbine system

NASA Astrophysics Data System (ADS)

Azizi, Mohammad Ali; Brouwer, Jacob

2017-10-01

A better understanding of turbulent unsteady flows in gas turbine systems is necessary to design and control compressors for hybrid fuel cell-gas turbine systems. Compressor stall/surge analysis for a 4 MW hybrid solid oxide fuel cell-gas turbine system for locomotive applications is performed based upon a 1.7 MW multi-stage air compressor. Control strategies are applied to prevent operation of the hybrid SOFC-GT beyond the stall/surge lines of the compressor. Computational fluid dynamics tools are used to simulate the flow distribution and instabilities near the stall/surge line. The results show that a 1.7 MW system compressor like that of a Kawasaki gas turbine is an appropriate choice among the industrial compressors to be used in a 4 MW locomotive SOFC-GT with topping cycle design. The multi-stage radial design of the compressor enhances the ability of the compressor to maintain air flow rate during transient step-load changes. These transient step-load changes are exhibited in many potential applications for SOFC/GT systems. The compressor provides sustained air flow rate during the mild stall/surge event that occurs due to the transient step-load change that is applied, indicating that this type of compressor is well-suited for this hybrid application.

Hydraulic transients in the long diversion-type hydropower station with a complex differential surge tank.

PubMed

Yu, Xiaodong; Zhang, Jian; Zhou, Ling

2014-01-01

Based on the theory of hydraulic transients and the method of characteristics (MOC), a mathematic model of the differential surge tank with pressure-reduction orifices (PROs) and overflow weirs for transient calculation is proposed. The numerical model of hydraulic transients is established using the data of a practical hydropower station; and the probable transients are simulated. The results show that successive load rejection is critical for calculating the maximum pressure in spiral case and the maximum rotating speed of runner when the bifurcated pipe is converging under the surge tank in a diversion-type hydropower station; the pressure difference between two sides of breast wall is large during transient conditions, and it would be more serious when simultaneous load rejections happen after load acceptance; the reasonable arrangement of PROs on breast wall can effectively decrease the pressure difference.

Defining Flood Zone Transitions in Low-Gradient Coastal Regions

NASA Astrophysics Data System (ADS)

Bilskie, M. V.; Hagen, S. C.

2018-03-01

Worldwide, coastal, and deltaic communities are susceptible to flooding from the individual and combined effects of rainfall excess and astronomic tide and storm surge inundation. Such flood events are a present (and future) cause of concern as observed from recent storms such as the 2016 Louisiana flood and Hurricanes Harvey, Irma, and Maria. To assess flood risk across coastal landscapes, it is advantageous to first delineate flood transition zones, which we define as areas susceptible to hydrologic and coastal flooding and their collective interaction. We utilize numerical simulations combining rainfall excess and storm surge for the 2016 Louisiana flood to describe a flood transition zone for southeastern Louisiana. We show that the interaction of rainfall excess with coastal surge is nonlinear and less than the superposition of their individual components. Our analysis provides a foundation to define flooding zones across coastal landscapes throughout the world to support flood risk assessments.

Blade row dynamic digital compressor program. Volume 1: J85 clean inlet flow and parallel compressor models

NASA Technical Reports Server (NTRS)

Tesch, W. A.; Steenken, W. G.

1976-01-01

The results are presented of a one-dimensional dynamic digital blade row compressor model study of a J85-13 engine operating with uniform and with circumferentially distorted inlet flow. Details of the geometry and the derived blade row characteristics used to simulate the clean inlet performance are given. A stability criterion based upon the self developing unsteady internal flows near surge provided an accurate determination of the clean inlet surge line. The basic model was modified to include an arbitrary extent multi-sector parallel compressor configuration for investigating 180 deg 1/rev total pressure, total temperature, and combined total pressure and total temperature distortions. The combined distortions included opposed, coincident, and 90 deg overlapped patterns. The predicted losses in surge pressure ratio matched the measured data trends at all speeds and gave accurate predictions at high corrected speeds where the slope of the speed lines approached the vertical.

Hydraulic Transients in the Long Diversion-Type Hydropower Station with a Complex Differential Surge Tank

PubMed Central

Yu, Xiaodong; Zhang, Jian

2014-01-01

Based on the theory of hydraulic transients and the method of characteristics (MOC), a mathematic model of the differential surge tank with pressure-reduction orifices (PROs) and overflow weirs for transient calculation is proposed. The numerical model of hydraulic transients is established using the data of a practical hydropower station; and the probable transients are simulated. The results show that successive load rejection is critical for calculating the maximum pressure in spiral case and the maximum rotating speed of runner when the bifurcated pipe is converging under the surge tank in a diversion-type hydropower station; the pressure difference between two sides of breast wall is large during transient conditions, and it would be more serious when simultaneous load rejections happen after load acceptance; the reasonable arrangement of PROs on breast wall can effectively decrease the pressure difference. PMID:25133213

C-reactive protein induces release of both endothelial microparticles and circulating endothelial cells in vitro and in vivo: further evidence of endothelial dysfunction.

PubMed

Devaraj, Sridevi; Kumaresan, Pappanaicken R; Jialal, Ishwarlal

2011-12-01

Inflammation is pivotal in atherosclerosis. A key early event in atherosclerosis is endothelial dysfunction. C-reactive protein (CRP), the prototypic marker of inflammation in humans, is a risk marker for cardiovascular disease, and there is mounting evidence to support its role in atherothrombosis. CRP has been shown to promote endothelial dysfunction both in vitro and in vivo. Emerging biomarkers of endothelial dysfunction include circulating endothelial cells (CECs) and endothelial microparticles (EMPs). However, there is a paucity of data examining the effect of CRP on CEC and EMP production in vitro and in vivo. In this report, we treated human aortic endothelial cells (HAECs) with increasing concentrations of CRP (0-50 μg/mL) or boiled CRP. We counted CECs and EMPs by flow cytometry. Although CRP treatment resulted in a significant increase in release of both CECs and EMPs, boiled CRP failed to have an effect. Pretreatment of HAECs with sepiapterin or diethylenetriamine NONOate, both of which preserve nitric oxide (NO), resulted in attenuation of CRP's effects on CECs and EMPs. CD32 and CD64 blocking antibodies but not CD16 antibody or lectin-like oxidized LDL receptor 1 small interfering RNA (LOX-1 siRNA) prevented CRP-induced production of CECs and EMPs. Furthermore, delivery of human CRP to Wistar rats compared with human serum albumin resulted in significantly increased CECs and EMPs, corroborating the in vitro findings. We provide novel data that CRP, via NO deficiency, promotes endothelial dysfunction by inducing release of CECs and EMPs, which are biomarkers of endothelial dysfunction.

Strategies, Protections and Mitigations for Electric Grid from Electromagnetic Pulse Effects

DOE Office of Scientific and Technical Information (OSTI.GOV)

Foster, Rita Ann; Frickey, Steven Jay

2016-01-01

The mission of DOE’s Office of Electricity Delivery and Energy Reliability (OE) is to lead national efforts to modernize the electricity delivery system, enhance the security and reliability of America’s energy infrastructure and facilitate recovery from disruptions to the energy supply. One of the threats OE is concerned about is a high-altitude electro-magnetic pulse (HEMP) from a nuclear explosion and eletro-magnetic pulse (EMP) or E1 pulse can be generated by EMP weapons. DOE-OE provides federal leadership and technical guidance in addressing electric grid issues. The Idaho National Laboratory (INL) was chosen to conduct the EMP study for DOE-OE due tomore » its capabilities and experience in setting up EMP experiments on the electric grid and conducting vulnerability assessments and developing innovative technology to increase infrastructure resiliency. This report identifies known impacts to EMP threats, known mitigations and effectiveness of mitigations, potential cost of mitigation, areas for government and private partnerships in protecting the electric grid to EMP, and identifying gaps in our knowledge and protection strategies.« less

A review of mortality associated with elongate mineral particle (EMP) exposure in occupational epidemiology studies of gold, talc, and taconite mining.

PubMed

Mandel, Jeffrey H; Alexander, Bruce H; Ramachandran, Gurumurthy

2016-12-01

Mining of gold, taconite, and talc may involve exposure to elongate mineral particles (EMP). The involved EMPs are typically non-asbestiform, include dimensions that regulatory definitions exclude, and have been less studied. A review of the literature was undertaken for this exposure and occupational epidemiological studies that occur in gold, talc, and taconite mining. Quantitative EMP exposure information in these industries is incomplete. However, there are consistent findings of pneumoconiosis in each of these types of mining. A recent case-control study suggests a possible association between this exposure and mesothelioma. Lung cancer is inconsistently reported in these industries and is an unlikely outcome of non-asbestiform EMP exposure. There is evidence of cardiovascular mortality excess across all of these types of mining. Non-malignant respiratory disease and cardiovascular mortality have been consistently increased in these industries. Further investigation, including additional insights for the role of non-asbestiform EMP, is warranted. Am. J. Ind. Med. 59:1047-1060, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Black Swan Tropical Cyclones

NASA Astrophysics Data System (ADS)

Emanuel, K.; Lin, N.

2012-12-01

Virtually all assessments of tropical cyclone risk are based on historical records, which are limited to a few hundred years at most. Yet stronger TCs may occur in the future and at places that have not been affected historically. Such events lie outside the realm of historically based expectations and may have extreme impacts. Their occurrences are also often made explainable after the fact (e.g., Hurricane Katrina). We nickname such potential future TCs, characterized by rarity, extreme impact, and retrospective predictability, "black swans" (Nassim Nicholas Taleb, 2007). As, by definition, black swan TCs have yet to happen, statistical methods that solely rely on historical track data cannot predict their occurrence. Global climate models lack the capability to predict intense storms, even with a resolution as high as 14 km (Emanuel et al. 2010). Also, most dynamic downscaling methods (e.g., Bender et al. 2010) are still limited in horizontal resolution and are too expensive to implement to generate enough events to include rare ones. In this study, we apply a simpler statistical/deterministic hurricane model (Emanuel et al. 2006) to simulate large numbers of synthetic storms under a given (observed or projected) climate condition. The method has been shown to generate realistic extremes in various basins (Emanuel et al. 2008 and 2010). We also apply a hydrodynamic model (ADCIRC; Luettich et al. 1992) to simulate the storm surges generated by these storms. We then search for black swan TCs, in terms of the joint wind and surge damage potential, in the generated large databases. Heavy rainfall is another important TC hazard and will be considered in a future study. We focus on three areas: Tampa Bay in the U.S., the Persian Gulf, and Darwin in Australia. Tampa Bay is highly vulnerable to storm surge as it is surrounded by shallow water and low-lying lands, much of which may be inundated by a storm tide of 6 m. High surges are generated by storms with a broad spectrum of characteristics in our synthetic database, although no large surge has been recorded historically as only one moderate storm passed by the area. Tampa black swans are identified as those that move northward parallel to the west Florida coast with high intensities and resonant with the Florida-shelf edge waves to generate extreme surges up to 10 m in Tampa Bay. The Arabian Sea area has sea surface temperatures warm enough to support the development of severe TCs, but TC development has been limited by low humidity and high wind shear, and only one recorded TC (super cyclonic storm Gonu in 2007) moved close to the Persian Gulf, making landfall in Oman and Iran. Our analysis shows that black swan TCs can originate within the Persian Gulf and make landfall with high intensities in populous places; extreme surges over 9 m for Abu Dubai and Doha and over 7 m for Dubai are possible. Darwin experienced immense devastation from Cyclone Tracy of 1974, but the damage was mainly due to the strong winds (the surge was only about 1.6 m). Our analysis includes extremely intense black swan TCs that make landfall just south of Darwin, generating surges above 10 m; these results may prompt the city to reconsider its TC risk. We are currently analyzing the join probability of the extreme wind and surge of these black swan TCs to more clearly assess their full damage potentials.

[Effect of electromagnetic pulse irradiation on mice reproduction].

PubMed

Wang, Shui-ming; Wang, De-wen; Peng, Rui-yun; Chen, Hao-yu; Gao, Ya-bing; Cao, Xiao-zhe; Cui, Xue-mei; Zhao, Mei-lan

2003-10-01

To evaluate the effect of electromagnetic pulse (EMP) irradiation on mice reproduction. Female/male Kunming mice, 6 - 8 weeks old, prior to mating, or female after pregnancy were treated with whole body irradiation by 6 x 10(4) V/m electromagnetic pulse (EMP) for five times. The pregnant mice were killed on the 18th days, and teratological markers were analysed. EMP irradiation caused no significant changes in most of female organ weight and organ/body weight ratio. But it caused significant shortening in tail length of live foetus in the female mice before conception (prior to mating) or after pregnancy (P < 0.05), and obvious decrease in male offspring ratio (0.85 +/- 0.09 vs 1.09 +/- 0.17, P < 0.05). The male offspring ratio also significantly decreased (0.76 +/- 0.18 vs 1.09 +/- 0.17, P < 0.01) after male mice irradiated by EMP. The tail length of live foetus was shortened and male offspring sex ratio was increased after both male and female mice were irradiated by EMP. EMP irradiation also caused a significantly higher fetal death rate than normal control (P < 0.05). The embryo absorption rate was increased after irradiation except that was decreased in male mice. EMP irradiation has effect on pregnancy and offspring development in both male and female mice before mating and in female mice after pregnancy.

Occurrence of Emerging Micropollutants in Water Systems in Gauteng, Mpumalanga, and North West Provinces, South Africa.

PubMed

Wanda, Elijah M M; Nyoni, Hlengilizwe; Mamba, Bhekie B; Msagati, Titus A M

2017-01-13

The ubiquitous occurrence of emerging micropollutants (EMPs) in water is an issue of growing environmental-health concern worldwide. However, there remains a paucity of data regarding their levels and occurrence in water. This study determined the occurrence of EMPs namely: carbamazepine (CBZ), galaxolide (HHCB), caffeine (CAF), tonalide (AHTN), 4-nonylphenol (NP), and bisphenol A (BPA) in water from Gauteng, Mpumalanga, and North West provinces, South Africa using comprehensive two-dimensional gas chromatography coupled to high resolution time-of-flight mass spectrometry (GCxGC-HRTOFMS). Kruskal-Wallis test and ANOVA were performed to determine temporal variations in occurrence of the EMPs. Principal component analysis (PCA) and Surfer Golden Graphics software for surface mapping were used to determine spatial variations in levels and occurrence of the EMPs. The mean levels ranged from 11.22 ± 18.8 ng/L for CAF to 158.49 ± 662 ng/L for HHCB. There was no evidence of statistically significant temporal variations in occurrence of EMPs in water. Nevertheless, their levels and occurrence vary spatially and are a function of two principal components (PCs, PC1 and PC2) which controlled 89.99% of the variance. BPA was the most widely distributed EMP, which was present in 62% of the water samples. The detected EMPs pose ecotoxicological risks in water samples, especially those from Mpumalanga province.

Occurrence of Emerging Micropollutants in Water Systems in Gauteng, Mpumalanga, and North West Provinces, South Africa

PubMed Central

Wanda, Elijah M. M.; Nyoni, Hlengilizwe; Mamba, Bhekie B.; Msagati, Titus A. M.

2017-01-01

The ubiquitous occurrence of emerging micropollutants (EMPs) in water is an issue of growing environmental-health concern worldwide. However, there remains a paucity of data regarding their levels and occurrence in water. This study determined the occurrence of EMPs namely: carbamazepine (CBZ), galaxolide (HHCB), caffeine (CAF), tonalide (AHTN), 4-nonylphenol (NP), and bisphenol A (BPA) in water from Gauteng, Mpumalanga, and North West provinces, South Africa using comprehensive two-dimensional gas chromatography coupled to high resolution time-of-flight mass spectrometry (GCxGC-HRTOFMS). Kruskal-Wallis test and ANOVA were performed to determine temporal variations in occurrence of the EMPs. Principal component analysis (PCA) and Surfer Golden Graphics software for surface mapping were used to determine spatial variations in levels and occurrence of the EMPs. The mean levels ranged from 11.22 ± 18.8 ng/L for CAF to 158.49 ± 662 ng/L for HHCB. There was no evidence of statistically significant temporal variations in occurrence of EMPs in water. Nevertheless, their levels and occurrence vary spatially and are a function of two principal components (PCs, PC1 and PC2) which controlled 89.99% of the variance. BPA was the most widely distributed EMP, which was present in 62% of the water samples. The detected EMPs pose ecotoxicological risks in water samples, especially those from Mpumalanga province. PMID:28098799

Disrupting Androgen Receptor Signaling Induces Snail-Mediated Epithelial-Mesenchymal Plasticity in Prostate Cancer.

PubMed

Miao, Lu; Yang, Lin; Li, Rui; Rodrigues, Daniel N; Crespo, Mateus; Hsieh, Jer-Tsong; Tilley, Wayne D; de Bono, Johann; Selth, Luke A; Raj, Ganesh V

2017-06-01

Epithelial-to-mesenchymal plasticity (EMP) has been linked to metastasis, stemness, and drug resistance. In prostate cancer, EMP has been associated with both suppression and activation of the androgen receptor (AR) signaling. Here we investigated the effect of the potent AR antagonist enzalutamide on EMP in multiple preclinical models of prostate cancer and patient tissues. Enzalutamide treatment significantly enhanced the expression of EMP drivers (ZEB1, ZEB2, Snail, Twist, and FOXC2) and mesenchymal markers (N-cadherin, fibronectin, and vimentin) in prostate cancer cells, enhanced prostate cancer cell migration, and induced prostate cancer transformation to a spindle, fibroblast-like morphology. Enzalutamide-induced EMP required concomitant suppression of AR signaling and activation of the EMP-promoting transcription factor Snail, as evidenced by both knockdown and overexpression studies. Supporting these findings, AR signaling and Snail expression were inversely correlated in C4-2 xenografts, patient-derived castration-resistant metastases, and clinical samples. For the first time, we elucidate a mechanism explaining the inverse relationship between AR and Snail. Specifically, we found that AR directly repressed SNAI1 gene expression by binding to specific AR-responsive elements within the SNAI1 promoter. Collectively, our findings demonstrate that de-repression of Snail and induction of EMP is an adaptive response to enzalutamide with implications for therapy resistance. Cancer Res; 77(11); 3101-12. ©2017 AACR . ©2017 American Association for Cancer Research.

Evaluation of Pharmacist Impact on Culture Review Process for Patients Discharged From the Emergency Department.

PubMed

Santiago, Ruben D; Bazan, Jose A; Brown, Nicole V; Adkins, Eric J; Shirk, Mary Beth

2016-10-01

Background: Accurate and timely review of microbiological test results is a core component of antimicrobial stewardship. There is documented success of these programs in the inpatient setting; however, emergency department (ED) patients are typically not included in these initiatives. Objectives: To assess the impact of an emergency medicine pharmacist (EMP)-facilitated review process of positive microbiological test results from patients discharged from the ED as measured by time to positive result review and number of indicated interventions completed. Methods: This was a retrospective study that compared EMP-facilitated to ED charge nurse (CN)-facilitated physician review of randomly selected positive microbiological test results. Groups were compared concurrently within the time frame of July 1, 2012 through December 31, 2012. Results: One hundred seventy-eight positive microbiological test results were included (EMP, n = 91; CN, n = 87). The median (IQR) time to initial review was 3 (1.0-6.3) hours for the EMP and 2 (0.3-5.5) hours for the CN group ( p = .35). Four percent (1/25) of indicated interventions were not completed in the EMP group versus 47% (14/30) in the CN group ( p = .0004). Conclusion: An EMP was significantly less likely to miss an intervention when indicated with no difference in time to review of positive microbiological results. These findings support the role of the EMP in antimicrobial stewardship in the ED.

Stakeholder perceptions of Environmental Management Plans as an environmental protection tool for major developments in the UK

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bennett, Sophie; Kemp, Simon; Hudson, Malcolm D., E-mail: mdh@soton.ac.uk

Insufficient implementation and the lack of legislative requirements for follow-up measures following the approval of projects are consistently highlighted as major shortcomings of Environmental Impact Assessment (EIA). Although adopted over 15 years ago by the World Bank, Environmental Management Plans (EMPs) were only semi-formalised in the UK in 2008 and arguably provide a continuous link or ‘bridge’ between the EIA process pre-consent and an Environmental Management System (EMS) post-consent. Drawing on twenty-one semi-structured interviews with stakeholders and thematic analysis of their responses, and a broad-scale practitioner survey, this study aimed to assess the effectiveness of EMPs as an environmental protectionmore » tool across the project lifecycle for major developments. The findings revealed a mixed picture of EMP effectiveness in practice, with EMPs only partially fulfilling a bridging role between EIA and EMS. There is no ‘gold standard’ terminology for EMPs, all having slightly different uses, thus presenting different focuses to different stakeholders and further enhancing variation in practice. For many stakeholders, the effectiveness was simply not known, due to the lack of communication and follow-up that still exists. EMP–EMS linkages were shown to be effective from the developer's perspective when a single organisation has involvement across all project phases, though weaknesses occur when multiple parties are involved. Among other stakeholders, knowledge varied significantly; whilst some were in agreement that the linkages worked, many were unaware of the connections and thought of them as two quite separate tools. Stakeholders advocated for the need to make EMPs a legal requirement; for improved communication between stakeholders during EMP implementation and increased documentation of project outcomes; and for EMPs to be consistently written by environmental professionals. Furthermore, weak links in the current process may be improved by providing detailed guidance for organisations on the potential for EMP–EMS linkages, with the additional aim of encouraging stakeholders to broaden their current specialist knowledge on environmental protection tools. - Highlights: • We assessed the effectiveness of Environmental Management Plans (EMPs) for major developments, focusing on delivery of mitigation and monitoring. • EIA and Environmental Management Systems (EMS) post-consent are weakly linked. • The use of EMPs varies significantly in practise, partly due to the range of terminology used. • Linkages are hampered by poor communication and follow-up • More detailed guidance on how EMPs can successfully link with EMS is required.« less

Racial differences in tumor necrosis factor-α-induced endothelial microparticles and interleukin-6 production

PubMed Central

Brown, Michael D; Feairheller, Deborah L; Thakkar, Sunny; Veerabhadrappa, Praveen; Park, Joon-Young

2011-01-01

African Americans (AA) tend to have heightened systemic inflammation and endothelial dysfunction. Endothelial microparticles (EMP) are released from activated/apoptotic endothelial cells (EC) when stimulated by inflammation. The purpose of our study was to assess EMP responses to inflammatory cytokine (TNF-α) and antioxidant (superoxide dismutase, SOD) conditions in human umbilical vein ECs (HUVECs) obtained from AA and Caucasians. EMPs were measured under four conditions: control (basal), TNF-α, SOD, and TNF-α + SOD. Culture supernatant was collected for EMP analysis by flow cytometry and IL-6 assay by ELISA. IL-6 protein expression was assessed by Western blot. AA HUVECs had greater EMP levels under the TNF-α condition compared to the Caucasian HUVECs (6.8 ± 1.1 vs 4.7% ± 0.4%, P = 0.04). The EMP level increased by 89% from basal levels in the AA HUVECs under the TNF-α condition (P = 0.01) compared to an 8% increase in the Caucasian HUVECs (P = 0.70). Compared to the EMP level under the TNF-α condition, the EMP level in the AA HUVECs was lower under the SOD only condition (2.9% ± 0.3%, P = 0.005) and under the TNF-α + SOD condition (2.1% ± 0.4%, P = 0.001). Basal IL-6 concentrations were 56.1 ± 8.8 pg/mL/μg in the AA and 30.9 ± 14.9 pg/mL/μg in the Caucasian HUVECs (P = 0.17), while basal IL-6 protein expression was significantly greater (P < 0.05) in the AA HUVECs. These preliminary observational results suggest that AA HUVECs may be more susceptible to the injurious effects of the proinflammatory cytokine, TNF-α. PMID:21966220

Electromagnetic pulse exposure induces overexpression of beta amyloid protein in rats.

PubMed

Jiang, Da-peng; Li, Jing; Zhang, Jie; Xu, Sheng-long; Kuang, Fang; Lang, Hai-yang; Wang, Ya-feng; An, Guang-zhou; Li, Jin-hui; Guo, Guo-zhen

2013-04-01

With the developing and widely used electromagnetic field (EMF) technology, more and more studies are focusing on the relationship between EMF and Alzheimer's disease (AD). Electromagnetic pulse (EMP) is one type of widely used EMF. This study aimed to clarify whether EMP exposure could induce cognitive and memory impairment, thus finding a possible relationship between EMP and AD. Forty healthy male Sprague Dawley rats were randomly divided into four groups. Animals, respectively, received 100, 1000, and 10,000 pulses EMP (field strength 50 kV/m, repetition rate 100 Hz) exposure and sham exposure when 2 months old. Monthly Morris water maze (MWM) was used to test the changes of cognitive and memory ability. Superoxide dismutase (SOD) activity and glutathione (GSH) content were used as oxidative stress indexes. Expressions of some types of Alzheimer's disease-related proteins were also detected. After exposure, EMP exposure caused clear cognitive and memory impairment compared with sham exposure group (p <0.05). Determination of oxidation indexes showed decreased SOD activity and GSH content in exposure groups compared with sham group. Immunohistochemical (IHC) staining showed increased beta amyloid protein (Aβ) in EMP exposure groups compared with sham group. Western blot experiments showed increased expressions of Aβ oligomer and beta amyloid protein precursor (APP) in EMP exposure groups. Increased expression of microtubule-associated protein 1 light chain 3-II (LC3-II) was also found. The present results showed that EMP exposure can cause long-term impairment in impaired cognition and memory of rats, resulting in AD-like symptoms. This may be induced by enhancing oxidative stress and is related to autophagy dysfunction. Copyright © 2013 IMSS. Published by Elsevier Inc. All rights reserved.

A Plasmodium falciparum PHIST protein binds the virulence factor PfEMP1 and comigrates to knobs on the host cell surface

PubMed Central

Oberli, Alexander; Slater, Leanne M.; Cutts, Erin; Brand, Françoise; Mundwiler-Pachlatko, Esther; Rusch, Sebastian; Masik, Martin F. G.; Erat, Michèle C.; Beck, Hans-Peter; Vakonakis, Ioannis

2014-01-01

Uniquely among malaria parasites, Plasmodium falciparum-infected erythrocytes (iRBCs) develop membrane protrusions, known as knobs, where the parasite adhesion receptor P. falciparum erythrocyte membrane protein 1 (PfEMP1) clusters. Knob formation and the associated iRBC adherence to host endothelium are directly linked to the severity of malaria and are functional manifestations of protein export from the parasite to the iRBC. A family of exported proteins featuring Plasmodium helical interspersed subtelomeric (PHIST) domains has attracted attention, with members being implicated in host-parasite protein interactions and differentially regulated in severe disease and among parasite isolates. Here, we show that PHIST member PFE1605w binds the PfEMP1 intracellular segment directly with Kd = 5 ± 0.6 μM, comigrates with PfEMP1 during export, and locates in knobs. PHIST variants that do not locate in knobs (MAL8P1.4) or bind PfEMP1 30 times more weakly (PFI1780w) used as controls did not display the same pattern. We resolved the first crystallographic structure of a PHIST protein and derived a partial model of the PHIST-PfEMP1 interaction from nuclear magnetic resonance. We propose that PFE1605w reinforces the PfEMP1-cytoskeletal connection in knobs and discuss the possible role of PHIST proteins as interaction hubs in the parasite exportome.—Oberli, A., Slater, L. M., Cutts, E., Brand, F., Mundwiler-Pachlatko, E., Rusch, S., Masik, M. F. G., Erat, M. C., Beck, H.-P., Vakonakis, I. A Plasmodium falciparum PHIST protein binds the virulence factor PfEMP1 and comigrates to knobs on the host cell surface. PMID:24983468

A Survey of Graduates of Combined Emergency Medicine-Pediatrics Residency Programs: An Update.

PubMed

Strobel, Ashley M; Chasm, Rose M; Woolridge, Dale P

2016-10-01

In 1998, emergency medicine-pediatrics (EM-PEDS) graduates were no longer eligible for the pediatric emergency medicine (PEM) sub-board certification examination. There is a paucity of guidance regarding the various training options for medical students who are interested in PEM. We sought to to determine attitudes and personal satisfaction of graduates from EM-PEDS combined training programs. We surveyed 71 graduates from three EM-PEDS residences in the United States. All respondents consider their combined training to be an asset when seeking a job, 92% find it to be an asset to their career, and 88% think it provided added flexibility to job searches. The most commonly reported shortcoming was their ineligibility for the PEM sub-board certification. The lack of this designation was perceived to be a detriment to securing academic positions in dedicated children's hospitals. When surveyed regarding which training offers the better skill set for the practice of PEM, 90% (44/49) stated combined EM-PEDS training. When asked which training track gives them the better professional advancement in PEM, 52% (23/44) chose combined EM-PEDS residency, 27% (12/44) chose a pediatrics residency followed by a PEM fellowship, and 25% (11/44) chose an EM residency then a PEM fellowship. No EM-PEDS respondents considered PEM fellowship training after the completion of the dual training program. EM-PEDS graduates found combined training to be an asset in their career. They felt that it provided flexibility in job searches, and that it was ideal training for the skill set required for the practice of PEM. EM-PEDS graduates' practices varied, including mixed settings, free-standing children's hospitals, and community emergency departments. Copyright © 2016 Elsevier Inc. All rights reserved.

Long-term electromagnetic pulse exposure induces Abeta deposition and cognitive dysfunction through oxidative stress and overexpression of APP and BACE1.

PubMed

Jiang, Da-Peng; Li, Jin-Hui; Zhang, Jie; Xu, Sheng-Long; Kuang, Fang; Lang, Hai-Yang; Wang, Ya-Feng; An, Guang-Zhou; Li, Jing; Guo, Guo-Zhen

2016-07-01

A progressively expanded literature has been devoted in the past years to the noxious or beneficial effects of electromagnetic field (EMF) to Alzheimer׳s disease (AD). This study concerns the relationship between electromagnetic pulse (EMP) exposure and the occurrence of AD in rats and the underlying mechanisms, focusing on the role of oxidative stress (OS). 55 healthy male Sprague Dawley (SD) rats were used and received continuous exposure for 8 months. Morris water maze (MWM) test was conducted to test the ability of cognitive and memory. The level of OS was detected by superoxide dismutase (SOD) activity and glutathione (GSH) content. We found that long-term EMP exposure induced cognitive damage in rats. The content of β-amyloid (Aβ) protein in hippocampus was increased after long-term EMP exposure. OS of hippocampal neuron was detected. Western blotting and immunohistochemistry (IHC) assay showed that the content of Aβ protein and its oligomers in EMP-exposed rats were higher than that of sham-exposed rats. The content of Beta Site App Cleaving Enzyme (BACE1) and microtubule-associated protein 1 light chain 3-II (LC3-II) in EMP-exposed rats hippocampus were also higher than that of sham-exposed rats. SOD activity and GSH content in EMP-exposed rats were lower than sham-exposed rats (p<0.05). Several mechanisms were proposed based on EMP exposure-induced OS, including increased amyloid precursor protein (APP) aberrant cleavage. Although further study is needed, the present results suggest that long-term EMP exposure is harmful to cognitive ability in rats and could induce AD-like pathological manifestation. Copyright © 2016 Elsevier B.V. All rights reserved.

Microparticles released by vascular endothelial cells increase hypoxia inducible factor expression in human proximal tubular HK-2 cells.

PubMed

Fernandez-Martínez, Ana Belen; Torija, Ana Valdehita; Carracedo, Julia; Ramirez, Rafael; de Lucio-Cazaña, Francisco Javier

2014-08-01

Microparticles are produced by vesiculation of the cell plasma membrane and serve as vectors of cell-to-cell communication. Co-culture experiments have shown that hypoxia-inducible factor-α (HIF-α)-regulated-genes are up-regulated in human renal proximal tubular HK-2 cells by endothelial cell factors which might be transported inside endothelial microparticles (EMP). Here we aimed to study in HK-2 cells the effect of EMP, produced by activated endothelial cells, on HIF-α and HIF-α-regulated vascular endothelial growth factor-A (VEGF-A). EMP, at a concentration much lower than that found in plasma, increased the expression of HIF-α/VEGF-A in a COX-2/EP2 receptor dependent manner. Since the EMP/cells ratio was ∼1/1000, we hypothesized that paracrine mediators produced by HK-2 cells amplified the initial signal. This hypothesis was confirmed by two facts which also suggested that the mediators were conveyed by particles released by HK-2 cells: (i) HIF-α was up-regulated in HK-2 cells treated with the pellet obtained from the conditioned medium of the EMP-treated HK-2 cells. (ii) In transwell experiments, EMP-treated cells increased the expression of HIF-α in untreated HK-2 cells. Interestingly, we detected these cells, particles that were released by EMP-treated HK-2 cells. Depending on the pathological context, activation of HIF-α and VEGF-A signaling in renal tissue/cells may have either beneficial or harmful effects. Therefore, our results suggest that their presence in the urinary space of EMP produced by activated endothelial cells may influence the outcome of a number of renal diseases. Copyright © 2014 Elsevier Ltd. All rights reserved.

Electromagnetic pulse reduces free radical generation in rat liver mitochondria in vitro.

PubMed

Wang, C; Zhou, H; Peng, R; Wang, L; Su, Z; Chen, P; Wang, S; Wang, S; Liu, Y; Cong, J; Wu, K; Hu, X; Fan, E

2013-04-01

Non-ionizing radiation electromagnetic pulse (EMP) is generally recorded to induce the generation of free radicals in vivo. Though mitochondria are the primary site to produce free radicals, a rare report is designed to directly investigate the EMP effects on free radical generation at mitochondrial level. Thus the present work was designed to study how EMP induces free radical generation in rat liver mitochondria in vitro using electron paramagnetic resonance technique. Surprisingly, our data suggest that EMP prevents free radical generation by activating antioxidant enzyme activity and reducing oxygen consumption and therefore free radical generation. Electron spin resonance measurements clearly demonstrate that disordering of mitochondrial lipid fluidity and membrane proteins mobility are the underlying contributors to this decreased oxygen consumption. Therefore, our results suggest that EMP might hold the potentiality to be developed as a non-invasive means to benefit certain diseases.

Spectral and temporal characteristics of target current and electromagnetic pulse induced by nanosecond laser ablation

NASA Astrophysics Data System (ADS)

Krása, J.; De Marco, M.; Cikhardt, J.; Pfeifer, M.; Velyhan, A.; Klír, D.; Řezáč, K.; Limpouch, J.; Krouský, E.; Dostál, J.; Ullschmied, J.; Dudžák, R.

2017-06-01

The current balancing the target charging and the emission of transient electromagnetic pulses (EMP) driven by the interaction of a focused 1.315 μm iodine 300 ps PALS laser with metallic and plastic targets were measured with the use of inductive probes. It is experimentally proven that the duration of return target currents and EMPs is much longer than the duration of laser-target interaction. The laser-produced plasma is active after the laser-target interaction. During this phase, the target acts as a virtual cathode and the plasma-target interface expands. A double exponential function is used in order to obtain the temporal characteristics of EMP. The rise time of EMPs fluctuates in the range up to a few tens of nanoseconds. Frequency spectra of EMP and target currents are modified by resonant frequencies of the interaction chamber.

1-Ethyl-1-methyl piperidinium bis(trifluoromethanesulfonyl)imide as a co-solvent in Li-ion batteries

NASA Astrophysics Data System (ADS)

Kim, Ketack; Cho, Young-Hyun; Shin, Heon-Cheol

2013-03-01

1-Ethyl-1-methyl piperidinium bis(trifluoromethanesulfonyl)imide (EMP-TFSI) is an ionic liquid with a melting temperature of 85 °C. Although it is a solid salt, it shows good miscibility with carbonate solvents, which allows EMP-TFSI to be used as a co-solvent in these systems. Ethylene carbonate is another solid co-solvent used in Li-ion batteries. Due to its smaller cationic size, EMP-TFSI provides better conductivity as a co-solvent than 1-methyl-1-propyl piperidinium bis(trifluoromethanesulfonyl)imide (MPP-TFSI), which is the smallest room-temperature piperidinium liquid salt known. In cells with 50 wt% IL and 50 wt% carbonate electrolyte, an EMP-TFSI mixed electrolyte performs better than an MPP-TFSI mixed electrolyte. Additionally, the discharge capacity values obtained from rate capability tests carried out with mixed EMP-TFSI are as good as those conducted with a pure carbonate electrolyte.

Apollo guidance, navigation and control: Guidance system operations plans for manned LM earth orbital and lunar missions using Program COLOSSUS 3. Section 7: Erasable memory programs

NASA Technical Reports Server (NTRS)

Hamilton, M. H.

1972-01-01

Erasable-memory programs (EMPs) designed for the guidance computers used in the command (CMC) and lunar modules (LGC) are described. CMC programs are designated COLOSSUS 3, and the associated EMPs are identified by a three-digit number beginning with 5. LGC programs are designated LUMINARY 1E, and the associated EMPs are identified, with one exception, by a three-digit number beginning with 1. The exception is EMP 99. The EMPs vary in complexity from a simple flagbit setting to a long and intricate logical structure. They all, however, cause the computer to behave in a way not intended in the original design of the programs; they accomplish this off-nominal behavior by some alteration of erasable memory to interface with existing fixed-memory programs to effect a desired result.

Electromagnetic radiations from laser interaction with gas-filled Hohlraum

NASA Astrophysics Data System (ADS)

Yang, Ming; Yang, Yongmei; Li, Tingshuai; Yi, Tao; Wang, Chuanke; Liu, Shenye; Jiang, Shaoen; Ding, Yongkun

2018-01-01

The emission of intensive electromagnetic pulse (EMP) due to laser-target interactions at the ShenGuang-III laser facility has been evaluated by probes. EMP signals measured using the small discone antennas demonstrated two variation trends including a bilateral oscillation wave and a unilateral oscillation wave. The new trend of unilateral oscillation could be attributed to the hohlraum structure and low-Z gas in the hohlraum. The EMP waveform showed multiple peaks when the gas-filled hohlraum was shot by the high-power laser. Comparing the EMP signals with the verification of stimulated Raman scattering energy and hard x-ray energy spectrum, we found that the intensity of EMP signals decreased with the increase of the hohlraum size. The current results are expected to offer preliminary information to study physical processes on laser injecting gas-filled hohlraums in the National Ignition Facility implementation.

High resolution field monitoring in coastal wetlands of the U.S. Mid-Atlantic to support quantification of storm surge attenuation at the regional scale

NASA Astrophysics Data System (ADS)

Paquier, A. E.; Haddad, J.; Lawler, S.; Garzon Hervas, J. L.; Ferreira, C.

2015-12-01

Hurricane Sandy (2012) demonstrated the vulnerability of the US East Coast to extreme events, and motivated the exploration of resilient coastal defenses that incorporate both hard engineering and natural strategies such as the restoration, creation and enhancement of coastal wetlands and marshes. Past laboratory and numerical studies have indicated the potential of wetlands to attenuate storm surge, and have demonstrated the complexity of the surge hydrodynamic interactions with wetlands. Many factors control the propagation of surge in these natural systems including storm characteristics, storm-induced hydrodynamics, landscape complexity, vegetation biomechanical properties and the interactions of these different factors. While previous field studies have largely focused on the impact of vegetation characteristics on attenuation processes, few have been undertaken with holistic consideration of these factors and their interactions. To bridge this gap of in-situ field data and to support the calibration of storm surge and wave numerical models such that wetlands can be correctly parametrized on a regional scale, we are carrying out high resolution surveys of hydrodynamics (pressure, current intensity and direction), morphology (topo-bathymetry, micro-topography) and vegetation (e.g. stem density, height, vegetation frontal area) in 4 marshes along the Chesapeake Bay. These areas are representative of the ecosystems and morphodynamic functions present in this region, from the tidal Potomac marshes to the barrier-island back-bays of the Delmarva Peninsula. The field monitoring program supports the investigation of the influence of different types of vegetation on water level, swell and wind wave attenuation and morphological evolution during storm surges. This dataset is also used to calibrate and validate numerical simulations of hurricane storm surge propagation at regional and local scales and to support extreme weather coastal resilience planning in the region. Figure 1 shows an area prone to storm surge impact within one of the 4 study sites: the Dameron Marsh Natural Area Preserve, located on the shoreline of the Northern Peninsula of Virginia, along the Chesapeake Bay.

Active identification and control of aerodynamic instabilities in axial and centrifugal compressors

NASA Astrophysics Data System (ADS)

Krichene, Assad

In this thesis, it is experimentally shown that dynamic cursors to stall and surge exist in both axial and centrifugal compressors using the experimental axial and centrifugal compressor rigs located in the School of Aerospace Engineering at the Georgia Institute of Technology. Further, it is shown that the dynamic cursors to stall and surge can be identified in real-time and they can be used in a simple control scheme to avoid the occurrence of stall and surge instabilities altogether. For the centrifugal compressor, a previously developed real-time observer is used in order to detect dynamic cursors to surge in real-time. An off-line analysis using the Fast Fourier Transform (FFT) of the open loop experimental data from the centrifugal compressor rig is carried out to establish the influence of compressor speed on the dynamic cursor frequency. The variation of the amplitude of dynamic cursors with compressor operating condition from experimental data is qualitatively compared with simulation results obtained using a generic compression system model subjected to white noise excitation. Using off-line analysis results, a simple control scheme based on fuzzy logic is synthesized for surge avoidance and recovery. The control scheme is implemented in the centrifugal compressor rig using compressor bleed as well as fuel flow to the combustor. Closed loop experimental results are obtained to demonstrate the effectiveness of the controller for both surge avoidance and surge recovery. The existence of stall cursors in an axial compression system is established using the observer scheme from off-line analysis of an existing database of a commercial gas turbine engine. However, the observer scheme is found to be ineffective in detecting stall cursors in the experimental axial compressor rig in the School of Aerospace Engineering at the Georgia Institute of Technology. An alternate scheme based on the amplitude of pressure data content at the blade passage frequency obtained using a pressure sensor located (in the casing) over the blade row is developed and used in the axial compressor rig for stall and surge avoidance and recovery. (Abstract shortened by UMI.)

Impact of climate change on New York City's coastal flood hazard: Increasing flood heights from the preindustrial to 2300 CE.

PubMed

Garner, Andra J; Mann, Michael E; Emanuel, Kerry A; Kopp, Robert E; Lin, Ning; Alley, Richard B; Horton, Benjamin P; DeConto, Robert M; Donnelly, Jeffrey P; Pollard, David

2017-11-07

The flood hazard in New York City depends on both storm surges and rising sea levels. We combine modeled storm surges with probabilistic sea-level rise projections to assess future coastal inundation in New York City from the preindustrial era through 2300 CE. The storm surges are derived from large sets of synthetic tropical cyclones, downscaled from RCP8.5 simulations from three CMIP5 models. The sea-level rise projections account for potential partial collapse of the Antarctic ice sheet in assessing future coastal inundation. CMIP5 models indicate that there will be minimal change in storm-surge heights from 2010 to 2100 or 2300, because the predicted strengthening of the strongest storms will be compensated by storm tracks moving offshore at the latitude of New York City. However, projected sea-level rise causes overall flood heights associated with tropical cyclones in New York City in coming centuries to increase greatly compared with preindustrial or modern flood heights. For the various sea-level rise scenarios we consider, the 1-in-500-y flood event increases from 3.4 m above mean tidal level during 1970-2005 to 4.0-5.1 m above mean tidal level by 2080-2100 and ranges from 5.0-15.4 m above mean tidal level by 2280-2300. Further, we find that the return period of a 2.25-m flood has decreased from ∼500 y before 1800 to ∼25 y during 1970-2005 and further decreases to ∼5 y by 2030-2045 in 95% of our simulations. The 2.25-m flood height is permanently exceeded by 2280-2300 for scenarios that include Antarctica's potential partial collapse. Copyright © 2017 the Author(s). Published by PNAS.

Impact of climate change on New York City’s coastal flood hazard: Increasing flood heights from the preindustrial to 2300 CE

PubMed Central

Mann, Michael E.; Emanuel, Kerry A.; Alley, Richard B.; Horton, Benjamin P.; DeConto, Robert M.; Donnelly, Jeffrey P.; Pollard, David

2017-01-01

The flood hazard in New York City depends on both storm surges and rising sea levels. We combine modeled storm surges with probabilistic sea-level rise projections to assess future coastal inundation in New York City from the preindustrial era through 2300 CE. The storm surges are derived from large sets of synthetic tropical cyclones, downscaled from RCP8.5 simulations from three CMIP5 models. The sea-level rise projections account for potential partial collapse of the Antarctic ice sheet in assessing future coastal inundation. CMIP5 models indicate that there will be minimal change in storm-surge heights from 2010 to 2100 or 2300, because the predicted strengthening of the strongest storms will be compensated by storm tracks moving offshore at the latitude of New York City. However, projected sea-level rise causes overall flood heights associated with tropical cyclones in New York City in coming centuries to increase greatly compared with preindustrial or modern flood heights. For the various sea-level rise scenarios we consider, the 1-in-500-y flood event increases from 3.4 m above mean tidal level during 1970–2005 to 4.0–5.1 m above mean tidal level by 2080–2100 and ranges from 5.0–15.4 m above mean tidal level by 2280–2300. Further, we find that the return period of a 2.25-m flood has decreased from ∼500 y before 1800 to ∼25 y during 1970–2005 and further decreases to ∼5 y by 2030–2045 in 95% of our simulations. The 2.25-m flood height is permanently exceeded by 2280–2300 for scenarios that include Antarctica’s potential partial collapse. PMID:29078274

Impact of climate change on New York City's coastal flood hazard: Increasing flood heights from the preindustrial to 2300 CE

NASA Astrophysics Data System (ADS)

Garner, Andra J.; Mann, Michael E.; Emanuel, Kerry A.; Kopp, Robert E.; Lin, Ning; Alley, Richard B.; Horton, Benjamin P.; DeConto, Robert M.; Donnelly, Jeffrey P.; Pollard, David

2017-11-01

The flood hazard in New York City depends on both storm surges and rising sea levels. We combine modeled storm surges with probabilistic sea-level rise projections to assess future coastal inundation in New York City from the preindustrial era through 2300 CE. The storm surges are derived from large sets of synthetic tropical cyclones, downscaled from RCP8.5 simulations from three CMIP5 models. The sea-level rise projections account for potential partial collapse of the Antarctic ice sheet in assessing future coastal inundation. CMIP5 models indicate that there will be minimal change in storm-surge heights from 2010 to 2100 or 2300, because the predicted strengthening of the strongest storms will be compensated by storm tracks moving offshore at the latitude of New York City. However, projected sea-level rise causes overall flood heights associated with tropical cyclones in New York City in coming centuries to increase greatly compared with preindustrial or modern flood heights. For the various sea-level rise scenarios we consider, the 1-in-500-y flood event increases from 3.4 m above mean tidal level during 1970–2005 to 4.0–5.1 m above mean tidal level by 2080–2100 and ranges from 5.0–15.4 m above mean tidal level by 2280–2300. Further, we find that the return period of a 2.25-m flood has decreased from ˜500 y before 1800 to ˜25 y during 1970–2005 and further decreases to ˜5 y by 2030–2045 in 95% of our simulations. The 2.25-m flood height is permanently exceeded by 2280–2300 for scenarios that include Antarctica's potential partial collapse.

Integrated modeling of the dynamic meteorological and sea surface conditions during the passage of Typhoon Morakot

NASA Astrophysics Data System (ADS)

Lee, Han Soo; Yamashita, Takao; Hsu, John R.-C.; Ding, Fei

2013-01-01

In August 2009, Typhoon Morakot caused massive flooding and devastating mudslides in the southern Taiwan triggered by extremely heavy rainfall (2777 mm in 4 days) which occurred during its passage. It was one of the deadliest typhoons that have ever attacked Taiwan in recent years. In this study, numerical simulations are performed for the storm surge and ocean surface waves, together with dynamic meteorological fields such as wind, pressure and precipitation induced by Typhoon Morakot, using an atmosphere-waves-ocean integrated modelling system. The wave-induced dissipation stress from breaking waves, whitecapping and depth-induced wave breaking, is parameterized and included in the wave-current interaction process, in addition to its influence on the storm surge level in shallow water along the coast of Taiwan. The simulated wind and pressure field captures the characteristics of the observed meteorological field. The spatial distribution of the accumulated rainfall within 4 days, from 00:00 UTC 6 August to 00:00 UTC 10 August 2009, shows similar patterns as the observed values. The 4-day accumulated rainfall of 2777 mm at the A-Li Shan mountain weather station for the same period depicted a high correlation with the observed value of 2780 mm/4 days. The effects of wave-induced dissipation stress in the wave-current interaction resulted in increased surge heights on the relatively shallow western coast of Taiwan, where the bottom slope of the bathymetry ranges from mild to moderate. The results also show that wave-breaking has to be considered for accurate storm surge prediction along the east coast of Taiwan over the narrow bank of surf zone with a high horizontal resolution of the model domain.

Predicting Impacts of tropical cyclones and sea-Level rise on beach mouse habitat

USGS Publications Warehouse

Chen, Qin; Wang, Hongqing; Wang, Lixia; Tawes, Robert; Rollman, Drew

2014-01-01

Alabama beach mouse (ABM) (Peromyscus polionotus ammobates) is an important component of the coastal dune ecosystem along the Gulf of Mexico. Due to habitat loss and degradation, ABM is federally listed as an endangered species. In this study, we examined the impacts of storm surge and wind waves, which are induced by hurricanes and sea-level rise (SLR), on the ABM habitat on Fort Morgan Peninsula, Alabama, using advanced storm surge and wind wave models and spatial analysis tools in geographic information systems (GIS). Statistical analyses of the long-term historical data enabled us to predict the extreme values of winds, wind waves, and water levels in the study area at different return periods. We developed a series of nested domains for both wave and surge modeling and validated the models using field observations of surge hydrographs and high watermarks of Hurricane Ivan (2004). We then developed wave atlases and flood maps corresponding to the extreme wind, surge and waves without SLR and with a 0.5 m of SLR by coupling the wave and surge prediction models. The flood maps were then merged with a map of ABM habitat to determine the extent and location of habitat impacted by the 100-year storm with and without SLR. Simulation results indicate that more than 82% of ABM habitat would be inundated in such an extreme storm event, especially under SLR, making ABM populations more vulnerable to future storm damage. These results have aided biologists, community planners, and other stakeholders in the identification, restoration and protection of key beach mouse habitat in Alabama. Methods outlined in this paper could also be used to assist in the conservation and recovery of imperiled coastal species elsewhere.

Why does substorm-associated auroral surge travel westward?

NASA Astrophysics Data System (ADS)

Ebihara, Y.; Tanaka, T.

2018-01-01

A substorm is a long-standing unsolved issue in solar-terrestrial physics. One of the big challenges is to explain reasonably the evolution of the morphological structure of the aurora associated with the substorm. The sudden appearance of a bright aurora and an auroral surge traveling westward (westward traveling surge, WTS) are noticeable features of the aurora during the substorm expansion phase. By using a global magnetohydrodynamics (MHD) simulation, we obtained the following results regarding the WTS. When the interplanetary magnetic field turns southward, a persistent dynamo appears in the cusp/mantle region, driving the two-cell magnetospheric convection. Then, the substorm growth phase begins. When magnetic reconnection takes place in the magnetotail, plasma is accelerated earthward in the plasma sheet, and accelerated toward the equatorial plane in the lobe. The second dynamo appears in the near-Earth region, which is closely associated with the generation of the field-aligned current (FAC) on the nightside. When the FAC reaches the ionosphere, the aurora becomes bright, and the onset of the expansion phase begins. In the ionosphere, the conductivity is intensified in the bright aurora due to the precipitation of accelerated electrons. The conductivity gradient gives rise to the overflow of the Hall current, which acts as the third dynamo. The overflow results in the accumulation of space charge, which causes a divergent electric field. The divergent electric field generates a thin, structured upward FAC adjacent to the bright aurora. The opposite process takes place on the opposite side of the bright aurora. In short, the upward FAC increases (appearance of aurora) at the leading edge of the surge, and decreases (disappearance of aurora) at the trailing edge of the surge. By repeating these processes, the surge seems to travel westward.

EMP Interaction: Principles, Techniques and Reference Data (A Compleat concatenation of Technology from the EMP Interaction Notes). EMP Interaction 2-1

DTIC Science & Technology

1980-12-01

staff of Dikewood, Santa Monica, no. only has contributed a large amount of material to this document, but also proofread the typescript . In...assistance and skills in the preparation of the entire typescript . Dikewood Industries, Inc. K.S.H. LEE Santa Monica, California September, 1979 xJ

Healthcare vulnerabilities to electromagnetic pulse.

PubMed

Ross, Lenard H; Mihelic, F Matthew

2008-01-01

The U.S. healthcare system is particularly vulnerable to the effects of electromagnetic pulse (EMP) attack because of the system's technological sophistication, but while national defense planners prepare for the considerable threat that EMP poses, there has been little or no recognition of this threat within the US healthcare community, and neither has there been any significant healthcare planning to deal with such an eventuality. Recognition of the risk presented by EMP, and advance institution of appropriate strategies to mitigate its effects on the healthcare system, could enable the preservation of much of that system's function in the face of EMP-related disruptions, and will greatly further all-hazards disaster preparations.

Endothelial microparticles and vascular parameters in subjects with and without arterial hypertension and coronary artery disease.

PubMed

Sansone, Roberto; Baaken, Maximilian; Horn, Patrick; Schuler, Dominik; Westenfeld, Ralf; Amabile, Nicolas; Kelm, Malte; Heiss, Christian

2018-08-01

Endothelial microparticles (EMPs) are markers of endothelial injury and activation. The role of EMPs in arterial hypertension is not well understood and EMPs are increased both in arterial hypertension and coronary artery disease (CAD). The data presented here show EMPs as defined by CD31 + /41 - , CD62e + , and CD144 + surface markers and vascular hemodynamic parameters including office and central blood pressure, heart rate, aortic augmentation index, pulse wave velocity, flow-mediated dilation, nitroglycerin-mediated dilation, brachial artery diameter, hyperemic wall shear stress, and laser Doppler perfusion of the cutaneous microcirculation of normotensives and hypertensives with and without CAD.

sae is essential for expression of the staphylococcal adhesins Eap and Emp.

PubMed

Harraghy, Niamh; Kormanec, Jan; Wolz, Christiane; Homerova, Dagmar; Goerke, Christiane; Ohlsen, Knut; Qazi, Saara; Hill, Philip; Herrmann, Mathias

2005-06-01

Eap and Emp are two Staphylococcus aureus adhesins initially described as extracellular matrix binding proteins. Eap has since emerged as being important in adherence to and invasion of eukaryotic cells, as well as being described as an immunomodulator and virulence factor in chronic infections. This paper describes the mapping of the transcription start point of the eap and emp promoters. Moreover, using reporter-gene assays and real-time PCR in defined regulatory mutants, environmental conditions and global regulators affecting expression of eap and emp were investigated. Marked differences were found in expression of eap and emp between strain Newman and the 8325 derivatives SH1000 and 8325-4. Moreover, both genes were repressed in the presence of glucose. Analysis of expression of both genes in various regulatory mutants revealed that sarA and agr were involved in their regulation, but the data suggested that there were additional regulators of both genes. In a sae mutant, expression of both genes was severely repressed. sae expression was also reduced in the presence of glucose, suggesting that repression of eap and emp in glucose-containing medium may, in part, be a consequence of a decrease in expression of sae.

Mechanisms involved in the blood-testis barrier increased permeability induced by EMP.

PubMed

Wang, Xiao-Wu; Ding, Gui-Rong; Shi, Chang-Hong; Zeng, Li-Hua; Liu, Jun-Ye; Li, Jing; Zhao, Tao; Chen, Yong-Bin; Guo, Guo-Zhen

2010-09-30

The blood-testis barrier (BTB) plays an important role in male reproductive system. Lots of environmental stimulations can increase the permeability of BTB and then result in antisperm antibody (AsAb) generation, which is a key step in male immune infertility. Here we reported the results of male mice exposed to electromagnetic pulse (EMP) by measuring the expression of tight-junction-associated proteins (ZO-1 and Occludin), vimentin microfilaments, and transforming growth factor-beta (TGF-beta3) as well as AsAb level in serum. Male BALB/c mice were sham exposed or exposed to EMP at two different intensities (200kV/m and 400kV/m) for 200 pulses. The testes were collected at different time points after EMP exposure. Immunofluorescence histocytochemistry, western blotting, laser confocal microscopy and RT-PCR were used in this study. Compared with sham group, the expression of ZO-1 and TGF-beta3 significantly decreased accompanied with unevenly stained vimentin microfilaments and increased serum AsAb levels in EMP-exposed mice. These results suggest a potential BTB injury and immune infertility in male mice exposed to a certain intensity of EMP. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

Influence of sex on the number of circulating endothelial microparticles and microRNA expression in middle-aged adults.

PubMed

Bammert, Tyler D; Hijmans, Jamie G; Kavlich, Philip J; Lincenberg, Grace M; Reiakvam, Whitney R; Fay, Ryan T; Greiner, Jared J; Stauffer, Brian L; DeSouza, Christopher A

2017-08-01

What is the central question of this study? Are there sex-related differences in the number of circulating endothelial microparticles (EMPs) and microparticle microRNA expression in middle-aged adult humans? What is the main finding and its importance? Although the numbers of circulating endothelial microparticles do not differ between middle-aged men and women, there are sex-related differences in the expression of miR-125a in activation-derived EMPs and miR-34a in apoptosis-derived EMPs. Differences in circulating endothelial microparticle microRNA content may provide new insight into the sex-related disparity in the risk and prevalence of vascular disease in middle-aged adults. The aims of this study were to determine: (i) whether circulating concentrations of endothelial microparticles (EMPs) differ in middle-aged men compared with women; and (ii) whether there are sex-related differences in microRNA expression in EMPs. Peripheral blood was collected from 30 sedentary adults: 15 men (56 ± 6 years old) and 15 women (56 ± 5 years old). Endothelial microparticles were defined by markers of activation (CD62e + ) or apoptosis (CD31 + /CD42b - ) by flow cytometry. Expression of microRNA (miR-34a, 92a, 125a and 126) in activation- and apoptosis-derived EMPs was measured by RT-PCR. Circulating activation- (33 ± 31 versus 39 ± 35 microparticles μl -1 ) and apoptosis-derived EMPs (49 ± 54 versus 42 ± 43 microparticles μl -1 ) were not significantly different between men and women. Expression of miR-125a (2.23 ± 2.01 versus 6.95 ± 3.99 a.u.) was lower (∼215%; P < 0.05) in activation-derived EMPs, whereas expression of miR-34a (1.17 ± 1.43 versus 0.38 ± 0.35 a.u.) was higher (∼210%; P < 0.05) in apoptosis-derived EMPs from men compared with women. Expression of microRNA in circulating EMPs may provide new insight into sex-related differences in cardiovascular disease. © 2017 The Authors. Experimental Physiology © 2017 The Physiological Society.

Early chemo-dynamical evolution of dwarf galaxies deduced from enrichment of r-process elements

NASA Astrophysics Data System (ADS)

Hirai, Yutaka; Ishimaru, Yuhri; Saitoh, Takayuki R.; Fujii, Michiko S.; Hidaka, Jun; Kajino, Toshitaka

2017-04-01

The abundance of elements synthesized by the rapid neutron-capture process (r-process elements) of extremely metal-poor (EMP) stars in the Local Group galaxies gives us clues to clarify the early evolutionary history of the Milky Way halo. The Local Group dwarf galaxies would have similarly evolved with building blocks of the Milky Way halo. However, how the chemo-dynamical evolution of the building blocks affects the abundance of r-process elements is not yet clear. In this paper, we perform a series of simulations using dwarf galaxy models with various dynamical times and total mass, which determine star formation histories. We find that galaxies with dynamical times longer than 100 Myr have star formation rates less than 10-3 M⊙ yr-1 and slowly enrich metals in their early phase. These galaxies can explain the observed large scatters of r-process abundance in EMP stars in the Milky Way halo regardless of their total mass. On the other hand, the first neutron star merger appears at a higher metallicity in galaxies with a dynamical time shorter than typical neutron star merger times. The scatters of r-process elements mainly come from the inhomogeneity of the metals in the interstellar medium whereas the scatters of α-elements are mostly due to the difference in the yield of each supernova. Our results demonstrate that the future observations of r-process elements in EMP stars will be able to constrain the early chemo-dynamical evolution of the Local Group galaxies.

The mass spectrum of the first stars

DOE Office of Scientific and Technical Information (OSTI.GOV)

Susa, Hajime; Tominaga, Nozomu; Hasegawa, Kenji, E-mail: susa@konan-u.ac.jp

2014-09-01

We perform cosmological hydrodynamics simulations with non-equilibrium primordial chemistry to obtain 59 minihalos that host first stars. The obtained minihalos are used as the initial conditions of local three-dimensional radiation hydrodynamics simulations to investigate the formation of the first stars. We find that two-thirds of the minihalos host multiple stars, while the other third has single stars. The mass of the stars found in our simulations are in the range of 1 M {sub ☉} ≲ M ≲ 300 M {sub ☉}, peaking at several× 10 M {sub ☉}. Most of the very massive stars of ≳ 140 M {submore » ☉} are born as single stars, although not all of the single stars are very massive. We also find a few stars of ≲ 1 M {sub ☉} that are kicked by the gravitational three body interactions to the position distant from the center of mass. The frequency that a star forming minihalo contains a binary system is ∼50%. We also investigate the abundance pattern of the stellar remnants by summing up the contributions from the first stars in the simulations. Consequently, the pattern is compatible with that of the low metallicity damped Lyα systems or the extremely metal-poor (EMP) stars, if the mass spectrum obtained in our experiment is shifted to the low mass side by 0.2 dex. If we consider the case that an EMP star is born in the remnant of the individual minihalo without mixing with others, the chemical signature of the pair instability supernova is more prominent, because most of them are born as single stars.« less

Targeting epithelial-mesenchymal plasticity in cancer: clinical and preclinical advances in therapy and monitoring.

PubMed

Bhatia, Sugandha; Monkman, James; Toh, Alan Kie Leong; Nagaraj, Shivashankar H; Thompson, Erik W

2017-09-20

The concept of epithelial-mesenchymal plasticity (EMP), which describes the dynamic flux within the spectrum of phenotypic states that invasive carcinoma cells may reside, is being increasingly recognised for its role in cancer progression and therapy resistance. The myriad of events that are able to induce EMP, as well as the more recently characterised control loops, results in dynamic transitions of cancerous epithelial cells to more mesenchymal-like phenotypes through an epithelial-mesenchymal transition (EMT), as well as the reverse transition from mesenchymal phenotypes to an epithelial one. The significance of EMP, in its ability to drive local invasion, generate cancer stem cells and facilitate metastasis by the dissemination of circulating tumour cells (CTCs), highlights its importance as a targetable programme to combat cancer morbidity and mortality. The focus of this review is to consolidate the existing knowledge on the strategies currently in development to combat cancer progression via inhibition of specific facets of EMP. The prevalence of relapse due to therapy resistance and metastatic propensity that EMP endows should be considered when designing therapy regimes, and such therapies should synergise with existing chemotherapeutics to benefit efficacy. To further improve upon EMP-targeted therapies, it is imperative to devise monitoring strategies to assess the impact of such treatments on EMP-related phenomenon such as CTC burden, chemosensitivity/-resistance and micrometastasis in patients. © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

An experimental description of the flow in a centrifugal compressor from alternate stall to surge

NASA Astrophysics Data System (ADS)

Moënne-Loccoz, V.; Trébinjac, I.; Benichou, E.; Goguey, S.; Paoletti, B.; Laucher, P.

2017-08-01

The present paper gives the experimental results obtained in a centrifugal compressor stage designed and built by SAFRAN Helicopter Engines. The compressor is composed of inlet guide vanes, a backswept splittered unshrouded impeller, a splittered vaned radial diffuser and axial outlet guide vanes. Previous numerical simulations revealed a particular S-shape pressure rise characteristic at partial rotation speed and predicted an alternate flow pattern in the vaned radial diffuser at low mass flow rate. This alternate flow pattern involves two adjacent vane passages. One passage exhibits very low momentum and a low pressure recovery, whereas the adjacent passage has very high momentum in the passage inlet and diffuses efficiently. Experimental measurements confirm the S-shape of the pressure rise characteristic even if the stability limit experimentally occurs at higher mass flow than numerically predicted. At low mass flow the alternate stall pattern is confirmed thanks to the data obtained by high-frequency pressure sensors. As the compressor is throttled the path to instability has been registered and a first scenario of the surge inception is given. The compressor first experiences a steady alternate stall in the diffuser. As the mass flow decreases, the alternate stall amplifies and triggers the mild surge in the vaned diffuser. An unsteady behavior results from the interaction of the alternate stall and the mild surge. Finally, when the pressure gradient becomes too strong, the alternate stall blows away and the compressor enters into deep surge.

Objective rapid delineation of areas at risk from block-and-ash pyroclastic flows and surges

USGS Publications Warehouse

Widiwijayanti, C.; Voight, B.; Hidayat, D.; Schilling, S.P.

2009-01-01

Assessments of pyroclastic flow (PF) hazards are commonly based on mapping of PF and surge deposits and estimations of inundation limits, and/or computer models of varying degrees of sophistication. In volcanic crises a PF hazard map may be sorely needed, but limited time, exposures, or safety aspects may preclude fieldwork, and insufficient time or baseline data may be available for reliable dynamic simulations. We have developed a statistically constrained simulation model for block-and-ash type PFs to estimate potential areas of inundation by adapting methodology from Iverson et al. (Geol Soc America Bull 110:972-984, (1998) for lahars. The predictive equations for block-and-ash PFs are calibrated with data from several volcanoes and given by A = (0.05 to 0.1) V2/3, B = (35 to 40) V2/3, where A is cross-sectional area of inundation, B is planimetric area and V is deposit volume. The proportionality coefficients were obtained from regression analyses and comparison of simulations to mapped deposits. The method embeds the predictive equations in a GIS program coupled with DEM topography, using the LAHARZ program of Schilling (1998). Although the method is objective and reproducible, any PF hazard zone so computed should be considered as an approximate guide only, due to uncertainties on the coefficients applicable to individual PFs, the authenticity of DEM details, and the volume of future collapses. The statistical uncertainty of the predictive equations, which imply a factor of two or more in predicting A or B for a specified V, is superposed on the uncertainty of forecasting V for the next PF to descend a particular valley. Multiple inundation zones, produced by simulations using a selected range of volumes, partly accommodate these uncertainties. The resulting maps show graphically that PF inundation potentials are highest nearest volcano sources and along valley thalwegs, and diminish with distance from source and lateral distance from thalweg. The model does not explicitly consider dynamic behavior, which can be important. Ash-cloud surge impact limits must be extended beyond PF hazard zones and we provide several approaches to do this. The method has been used to supply PF and surge hazard maps in two crises: Merapi 2006; and Montserrat 2006-2007. ?? Springer-Verlag 2008.

Waterworks, a full-scale chemical exposure exercise: interrogating pediatric critical care surge capacity in an inner-city tertiary care medical center.

PubMed

Shah, Vikas S; Pierce, Lauren C; Roblin, Patricia; Walker, Sarah; Sergio, Marte N; Arquilla, Bonnie

2014-02-01

Pediatric Intensive Care Unit (PICU) resources are overwhelmed in disaster as the need to accommodate influx of critically-ill children is increased. A full-scale chlorine overexposure exercise was conducted by the New York Institute for All Hazard Preparedness (NYIAHP) to assess the appropriateness of response of Kings County Hospital Center's (KCHC's) PICU surge plan to an influx of critically-ill children. The primary endpoint that was assessed was the ability of the institution to follow the PICU surge plan, while secondary endpoints include the ability to provide appropriate medical management. Thirty-six actors/patients (medical students or emergency medicine residents) were educated on presentations and appropriate medical management of patients after a chlorine overexposure, as well as lectures on drill design and expected PICU surge response. Victims presented to the hospital after simulated accidental chlorine overexposure at a public pool. Twenty-two patients with 14 family members needed evaluation; nine of these patients would require PICU admission. Three of nine PICU patients were low-fidelity mannequins. In addition to the 36 actor/patient evaluators, each area had two to four expert evaluators (disaster preparedness experts) to assess appropriateness of global response. Patients were expected to receive standard of care. Appropriateness of medical decisions and treatment was assessed retrospectively with review of electronic medical record. The initial PICU census was three of seven; two of these patients were transferred to the general ward. Of the nine patients that required Intensive Care Unit (ICU) admission, six actor/patients were admitted to the PICU, one was admitted to the Surgical Intensive Care Unit (SICU), one went to the Operating Room (OR), and one was admitted to a monitored-surge general pediatric bed. The remaining 13 actor/patients were treated and released. Medical, nursing, and respiratory staffing in the PICU and the general ward were increased by two main mechanisms (extension of work hours and in-house recruitment of additional staff). Emergency Department (ED) staffing was artificially increased prior to the drill. With the exception of ocular fluid pH testing in patients with ocular pruritus, all necessary treatments were given; however, an unneeded albuterol treatment was administered to one patient. Chart review showed adequate discharge instructions in four of 13 patients. Nine patients without respiratory complaints in the ED were not instructed to observe for dyspnea. All patients were in the PICU or alternate locations within 90 minutes. Discussion The staff was well versed in the major details of KCHC's PICU surge plan, which allowed smooth transition of patient care from the ED to the PICU. The plan provided for a roadmap to achieve adequate medical, nursing, and respiratory therapists. Medical therapy was appropriate in the PICU; however, in the ED, patients with ocular complaints did not receive optimal care. In addition, written discharge instruction and educational material regarding chlorine overexposure to all patients were not consistently provided. The PICU surge plan was immediately accessible through the KCHC intranet; however, not all participants were cognizant of this fact; this decreased the efficiency with which the roadmap was followed. An exaggerated ED staff facilitated evaluation and transfer of patients. During disasters, the ability to surge is paramount and each hospital addresses it differently. Hospitals and departments have written surge plans, but there is no literature available which assesses the validity of said plans through a rigorous, structured, simulated disaster drill. This study is the first to assess validity and effectiveness of a hospital's PICU surge plan. Overall, the KCHC PICU surge plan was effective; however, several deficiencies (mainly in communication and patient education in the ED) were identified, and this will improve future response.

Effects of Electromagnetic Pulses on a Multilayered System

DTIC Science & Technology

2014-07-01

repeatable high - power generators . Repetitive EMP (REMP) is usually wideband with each pulse being composed of a wide range of frequencies. This larger...types of EMPs that are of concern regarding electronics and system infrastructures; they are high -altitude EMP (HEMP) generated from nuclear...ELECTROMAGNETIC PULSES ON A MULTILAYERED SYSTEM A. Upia, K. M. Burke, J. L. Zirnheld Energy Systems Institute, Department of Electrical Engineering

Differential Impact of Acute High-Intensity Exercise on Circulating Endothelial Microparticles and Insulin Resistance between Overweight/Obese Males and Females

PubMed Central

Durrer, Cody; Robinson, Emily; Wan, Zhongxiao; Martinez, Nic; Hummel, Michelle L.; Jenkins, Nathan T.; Kilpatrick, Marcus W.; Little, Jonathan P.

2015-01-01

Background An acute bout of exercise can improve endothelial function and insulin sensitivity when measured on the day following exercise. Our aim was to compare acute high-intensity continuous exercise (HICE) to high-intensity interval exercise (HIIE) on circulating endothelial microparticles (EMPs) and insulin sensitivity in overweight/obese men and women. Methods Inactive males (BMI = 30 ± 3, 25 ± 6 yr, n = 6) and females (BMI = 28 ± 2, 21 ± 3 yr, n = 7) participated in three experimental trials in a randomized counterbalanced crossover design: 1) No exercise control (Control); 2) HICE (20 min cycling @ just above ventilatory threshold); 3) HIIE (10 X 1-min @ ∼90% peak aerobic power). Exercise conditions were matched for external work and diet was controlled post-exercise. Fasting blood samples were obtained ∼18 hr after each condition. CD62E+ and CD31+/CD42b- EMPs were assessed by flow cytometry and insulin resistance (IR) was estimated by homeostasis model assessment (HOMA-IR). Results There was a significant sex X exercise interaction for CD62E+ EMPs, CD31+/CD42b- EMPs, and HOMA-IR (all P<0.05). In males, both HICE and HIIE reduced EMPs compared to Control (P≤0.05). In females, HICE increased CD62E+ EMPs (P<0.05 vs. Control) whereas CD31+/CD42b- EMPs were unaltered by either exercise type. There was a significant increase in HOMA-IR in males but a decrease in females following HIIE compared to Control (P<0.05). Conclusions Overweight/obese males and females appear to respond differently to acute bouts of high-intensity exercise. A single session of HICE and HIIE reduced circulating EMPs measured on the morning following exercise in males but in females CD62E+ EMPs were increased following HICE. Next day HOMA-IR paradoxically increased in males but was reduced in females following HIIE. Future research is needed to investigate mechanisms responsible for potential differential responses between males and females. PMID:25710559

Interaction of Shallow Cold Surges with Topography on Scales of 100-1000 Kilometers.

NASA Astrophysics Data System (ADS)

Toth, James John

1987-09-01

A shallow cold air mass is defined as one not extending to the top of the mountain ridge with which it interacts. The structure of such an airmass is examined using both observational data and a hydrostatic version of the Colorado State University Regional Atmospheric Modeling System. The prime constraint on a shallow cold surge is that the flow must ultimately be parallel to the mountain ridge. It is found that the effects of this constraint are altered significantly by surface sensible heat flux. Cold surges are slowed during the daylight hours, a result consistent with previous observational studies in Colorado east of the Continental Divide. Two case studies are described in detail, and several other events are cited. Since observations alone do not provide a complete description of diversion of the cold air by the mountain range, numerical model simulations provide additional insight into important mechanisms. A case study on 14 June 1985 is described using observational and model data. The model development of a deep boundary layer within the frontal baroclinic zone is consistent with the observations for this and other cases. This development is due to strong surface heating. Turning off the model shortwave radiation is seen to produce a rapid southward acceleration of the surface front, with very shallow cold air behind the front. Model simulations with specified surface temperature differences confirm the importance of upward heat flux from the surface in slowing the southward movement of the cold surge. It is concluded that the slowing is not due simply to the thermal wind developing in response to the heating of higher terrain to the west. Since surface heating is distributed over a deeper layer on the warm side of the temperature discontinuity, there is frontolysis at the surface. But this modification would develop even over flat terrain. Sloping terrain introduces additional effects. Heating at the western, upslope side of the cold surge inhibits the development of pressure gradients favorable to northerly flow. A second contribution comes from westerly winds at ridgetop level. These winds are heated over the higher terrain and flow downslope, further retarding the progression of the cold air at the surface.

Gel shift analysis of the empA promoter region in Vibrio anguillarum.

PubMed

Denkin, Steven M; Sekaric, Pedja; Nelson, David R

2004-10-29

The induction of metalloprotease encoded by empA in Vibrio anguillarum occurs at high cell density in salmon intestinal mucus. Previously we have shown that there are significant differences in empA expression in two strains of V. anguillarum, M93Sm and NB10. It is hypothesized that differences in empA regulation are due to differences in binding of regulatory elements. Two strains of V. anguillarum, M93Sm and NB10, were examined and compared for the presence of DNA regulatory proteins that bind to and control the empA promoter region. Gel mobility shift assays, using a digoxigenin (DIG)-labeled oligomer containing a lux box-like element and the promoter for empA, were done to demonstrate the presence of a DNA-binding protein. Protein extracts from NB10 cells incubated in Luria Bertani broth + 2% NaCl (LB20), nine salts solution + 200 microg/ml mucus (NSSM), 3M (marine minimal medium), or NSS resulted in a gel mobility shift. No gel mobility shift was seen when protein extracts from either LB20- or NSSM-grown M93Sm cells were mixed with the DIG-labeled empA oligomer. The azocasein assay detected protease activity in all incubation conditions for NB10 culture supernatants. In contrast, protease activity was detected in M93Sm culture supernatants only when incubated in NSSM. Since the luxR homologue in V. anguillarum, vanT, has been cloned, sequenced, and shown to be required for protease activity, we wanted to determine if vanT mutants of NB10 exhibit the same gel shift observed in the wild-type. Site-directed mutagenesis was used to create vanT mutants in V. anguillarum M93Sm and NB10 to test whether VanT is involved with the gel mobility shift. Both vanT mutants, M02 and NB02, did not produce protease activity in any conditions. However, protein extracts from NB02 incubated in each condition still exhibited a gel shift when mixed with the DIG-labeled empA oligomer. The data demonstrate that protein extracts of V. anguillarum NB10 cells contain a protein that binds to a 50 bp oligomer containing the empA promoter-lux box-like region. NB10 cells express empA during stationary phase in all growth conditions. The DNA binding protein is not present in M93Sm extracts. M93Sm cells express protease activity only when incubated at high cell density in fish gastrointestinal mucus. The gel shift observed with NB10 cells is not due to VanT binding. The data also suggest that the DNA binding protein is responsible for the less restrictive expression of empA in NB10 compared to M93Sm.

[The effects of electromagnetic pulse on fluidity and lipid peroxidation of mitochondrial membrane].

PubMed

Wang, Changzhen; Cong, Jianbo; Xian, Hong; Cao, Xiaozhe; Sun, Cunpu; Wu, Ke

2002-08-01

To study the effects of intense electromagnetic pulse(EMP) on the biological effects of mitochondrial membrane. Rat liver mitochondrial suspension was exposed to EMP at 60 kV/m level. The changes of membrane lipid fluidity and membrane protein mobility were detected by ESR and spin label technique. Malondialdehyde(MDA) was detected by spectrophotometer. The mobility of membrane protein decreased significantly(P < 0.05). Correlation time (tau c) of control group was (0.501 +/- 0.077) x 10(-9)s, and tau c of EMP group was (0.594 +/- 0.049) x 10(-9)s, indicating that the mobility of protein was restricted. The fluidity of mitochondrial membrane increased significantly(P < 0.05) at the same time. Order parameter(S) of mitochondrial membrane lipid in control group was 0.63 +/- 0.01, while S of EMP group was 0.61 +/- 0.01(P < 0.05). MDA decreased significantly. The mobility and lipid peroxidation of mitochondrial membrane may be disturbed after EMP exposure.

EMP-induced alterations of tight junction protein expression and disruption of the blood-brain barrier.

PubMed

Ding, Gui-Rong; Qiu, Lian-Bo; Wang, Xiao-Wu; Li, Kang-Chu; Zhou, Yong-Chun; Zhou, Yan; Zhang, Jie; Zhou, Jia-Xing; Li, Yu-Rong; Guo, Guo-Zhen

2010-07-15

The blood-brain barrier (BBB) is critical to maintain cerebral homeostasis. In this study, we examined the effects of exposure to electromagnetic pulse (EMP) on the functional integrity of BBB and, on the localization and expression of tight junction (TJ) proteins (occludin and ZO-1) in rats. Animals were sham or whole-body exposed to EMP at 200 kV/m for 400 pulses. The permeability of BBB in rat cerebral cortex was examined by using Evans Blue (EB) and lanthanum nitrate as vascular tracers. The localization and expression of TJ proteins were assessed by western blot and immunofluorescence analysis, respectively. The data indicated that EMP exposure caused: (i) increased permeability of BBB, and (ii) altered localization as well as decreased levels of TJ protein ZO-1. These results suggested that the alteration of ZO-1 may play an important role in the disruption of tight junctions, which may lead to dysfunction of BBB after EMP exposure. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

Recommended E3 HEMP Heave Electric Field Waveform for the Critical Infrastructures. Volume 2

DTIC Science & Technology

2017-07-31

OF THE COMMISSION TO ASSESS THE THREAT TO THE UNITED STATES FROM ELECTROMAGNETIC PULSE (EMP) ATTACK The cover photo depicts Fishbowl Starfish...Assess the Threat to the United States from Electromagnetic Pulse (EMP) Attack. The Commission was established by Congress in the FY2001 National...Department of Defense E electric field EMP electromagnetic pulse EPRI Electric Power Research Institute FERC Federal Energy Regulatory Commission GMD

Assessment and Mitigation of Electromagnetic Pulse (EMP) Impacts at Short-pulse Laser Facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, Jr., C G; Bond, E; Clancy, T

2009-10-02

The National Ignition Facility (NIF) will be impacted by electromagnetic pulse (EMP) during normal long-pulse operation, but the largest impacts are expected during short-pulse operation utilizing the Advanced Radiographic Capability (ARC). Without mitigation these impacts could range from data corruption to hardware damage. We describe our EMP measurement systems on Titan and NIF and present some preliminary results and thoughts on mitigation.

Assessment and Mitigation of Electromagnetic Pulse (EMP) Impacts at Short-pulse Laser Facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, Jr., C G; Bond, E; Clancy, T

2010-02-04

The National Ignition Facility (NIF) will be impacted by electromagnetic pulse (EMP) during normal long-pulse operation, but the largest impacts are expected during short-pulse operation utilizing the Advanced Radiographic Capability (ARC). Without mitigation these impacts could range from data corruption to hardware damage. We describe our EMP measurement systems on Titan and NIF and present some preliminary results and thoughts on mitigation.

Exploring the SDSS Dataset with Linked Scatter Plots: I. EMP, CEMP, and CV Stars

NASA Technical Reports Server (NTRS)

Carbon, Duane F.; Henze, Christopher; Nelson, Bron C.

2017-01-01

We present the results of a search for EMP, CEMP, and cataclysmic variable stars using a new exploration tool based on linked scatter plots (LSPs). Our approach is especially designed to work with very large spectrum data sets such as the SDSS, LAMOST, RAVE, and Gaia data sets and can be applied to stellar, galaxy, and quasar spectra. As a demonstration, we conduct a search for EMP, CEMP, and cataclysmic variable stars in the SDSS DR10 data set. We first created a 3326-dimensional phase space containing nearly 2 billion measures of the strengths of over 1600 spectral features in 569,738 SDSS stars. These measures capture essentially all the stellar atomic and molecular species visible at the resolution of SDSS spectra. We show how LSPs can be used to quickly isolate and examine interesting portions of this phase space. To illustrate, we use LSPs coupled with cuts in selected portions of phase space to extract EMP stars, C-rich EMP stars, and CV stars. We present identifications for 59 previously unrecognized candidate EMP stars and 11 previously unrecognized candidate CEMP stars. We also call attention to 2 candidate He II emission CV stars found by the LSP approach that have not yet been discussed in the literature.

CDC's Emergency Management Program activities - worldwide, 2003-2012.

PubMed

2013-09-06

In 2003, recognizing the increasing frequency and complexity of disease outbreaks and disasters and a greater risk for terrorism, CDC established the Emergency Operations Center (EOC), bringing together CDC staff members who respond to public health emergencies to enhance communication and coordination. To complement the physical EOC environment, CDC implemented the Incident Management System (IMS), a staffing structure and set of standard operational protocols and services to support and monitor CDC program-led responses to complex public health emergencies. The EOC and IMS are key components of CDC's Emergency Management Program (EMP), which applies emergency management principles to public health practice. To enumerate activities conducted by the EMP during 2003-2012, CDC analyzed data from daily reports and activity logs. The results of this analysis determined that, during 2003-2012, the EMP fully activated the EOC and IMS on 55 occasions to support responses to infectious disease outbreaks, natural disasters, national security events (e.g., conventions, presidential addresses, and international summits), mass gatherings (e.g., large sports and social events), and man-made disasters. On 109 other occasions, the EMP was used to support emergency responses that did not require full EOC activation, and the EMP also conducted 30 exercises and drills. This report provides an overview of those 194 EMP activities.

Exploring the SDSS Dataset with Linked Scatter Plots: I. EMP, CEMP, and CV Stars.

PubMed

Carbon, Duane F; Henze, Christopher; Nelson, Bron C

2017-02-01

We present the results of a search for EMP, CEMP, and cataclysmic variable stars using a new exploration tool based on linked scatter plots (LSPs). Our approach is especially designed to work with very large spectrum data sets such as the SDSS, LAMOST, RAVE, and Gaia data sets and can be applied to stellar, galaxy, and quasar spectra. As a demonstration, we conduct a search for EMP, CEMP, and cataclysmic variable stars in the SDSS DR10 data set. We first created a 3326-dimensional phase space containing nearly 2 billion measures of the strengths of over 1600 spectral features in 569,738 SDSS stars. These measures capture essentially all the stellar atomic and molecular species visible at the resolution of SDSS spectra. We show how LSPs can be used to quickly isolate and examine interesting portions of this phase space. To illustrate, we use LSPs coupled with cuts in selected portions of phase space to extract EMP stars, C-rich EMP stars, and CV stars. We present identifications for 59 previously unrecognized candidate EMP stars and 11 previously unrecognized candidate CEMP stars. We also call attention to 2 candidate He II emission CV stars found by the LSP approach that have not yet been discussed in the literature.

Nuclear pulse. I - Awakening to the chaos factor

NASA Astrophysics Data System (ADS)

Broad, W. J.

1981-05-01

The discovery of the significance of the high-voltage wave termed electromagnetic pulse (EMP), which occurs following the high-altitude detonation of a nuclear device, is discussed. The disruptions to the street lights, burglar alarms and circuit breakers of Hawaii caused by the detonation of a nuclear device 248 mi above Johnson Island in the Pacific in July, 1962 are described and attributed to the Compton electrons produced by the impact of gamma rays from the nuclear explosion on air in the upper atmosphere. It is pointed out, however, that at the time of the explosion, most communications systems were based on vacuum tube and electromechanical technology, which is about 10,000,000 times harder against EMP than integrated solid-state circuitry, and thus the threat posed by EMP to the power grid and communications capabilities was not apparent. Efforts undertaken to harden discrete (missile) and communications systems against EMP are outlined for the example of the Safeguard ABM system, and difficulties are pointed out. Soviet awareness of EMP is considered, and the discovery of vacuum tubes on board the state-of-the-art Foxbat MiG interceptor flown into Japan is noted as a possible indicator of this awareness. It is concluded that the problem of EMP will increase in significance as semiconductor electronics proliferates.

Exploring the SDSS Dataset with Linked Scatter Plots: I. EMP, CEMP, and CV Stars

PubMed Central

Carbon, Duane F.; Henze, Christopher; Nelson, Bron C.

2017-01-01

We present the results of a search for EMP, CEMP, and cataclysmic variable stars using a new exploration tool based on linked scatter plots (LSPs). Our approach is especially designed to work with very large spectrum data sets such as the SDSS, LAMOST, RAVE, and Gaia data sets and can be applied to stellar, galaxy, and quasar spectra. As a demonstration, we conduct a search for EMP, CEMP, and cataclysmic variable stars in the SDSS DR10 data set. We first created a 3326-dimensional phase space containing nearly 2 billion measures of the strengths of over 1600 spectral features in 569,738 SDSS stars. These measures capture essentially all the stellar atomic and molecular species visible at the resolution of SDSS spectra. We show how LSPs can be used to quickly isolate and examine interesting portions of this phase space. To illustrate, we use LSPs coupled with cuts in selected portions of phase space to extract EMP stars, C-rich EMP stars, and CV stars. We present identifications for 59 previously unrecognized candidate EMP stars and 11 previously unrecognized candidate CEMP stars. We also call attention to 2 candidate He II emission CV stars found by the LSP approach that have not yet been discussed in the literature. PMID:28684884

Iron-Regulated Biofilm Formation in Staphylococcus aureus Newman Requires ica and the Secreted Protein Emp▿

PubMed Central

Johnson, Miranda; Cockayne, Alan; Morrissey, Julie A.

2008-01-01

Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface and have previously been implicated in a number of aspects of S. aureus pathogenesis. We showed here that the transcription of these important virulence factors is induced by growth in low-iron medium, reflective of the in vivo environment. Our results show that iron regulation of Eap and Emp is Fur independent. However, Fur is required for full induction of eap and emp expression in low-iron conditions. In this study, we demonstrated that in addition to Fur, low-iron-induced biofilm formation requires Sae, Agr, and SarA. In iron-restricted growth conditions, Sae and Agr are essential for Emp and Eap expression and hence for biofilm formation, whereas SarA appears to have a less-significant role. We also showed that expression of the ica operon is required for biofilm formation in iron-restricted growth conditions. We demonstrated that in fact, ica is required for the expression of the important multifunctional virulence determinants eap and emp. PMID:18268030

Finishing broiler toms using an estradiol 17 beta implant together with a high energy-low protein final feed.

PubMed

Moran, E T; Etches, R J

1983-06-01

Wrolstad Small White toms were implanted with 10 mg of estradiol 17 beta monopalmitate (EMP) at 8 weeks of age. Common corn-soybean meal feeds were given through to 12 weeks, then one-half the birds from control and EMP groups received either an adequate (16% protein, 3166 kcal ME/kg) or high energy-low protein (HE-LP, 12%, 3373 kcal) feed to 14 weeks. No differences in weight gain and feed conversion occurred between EMP and control treatments at 12 weeks but at 14 weeks when the HE-LP diet had been fed the implanted birds performed better than controls. The HE-LP feed led to body weights and feed efficiencies below that of toms given adequate diet. In all cases, EMP elicited male secondary sex characteristics rather than feminization. Processing losses were increased with EMP and when the HE-LP feed had been given. Both treatments also improved finish assessment and were additive to the extent that a substantial increase in grade occurred. Effects on carcass composition, yield of commercial cuts, and cooking loss were small. Implantation, reduced meat yield percentage of breast and thigh. The increase in grade advantage from combining EMP with a feed that forced fat deposition more than compensated for the adverse effects.

A Study on Application of Fuzzy Adaptive Unscented Kalman Filter to Nonlinear Turbojet Engine Control

NASA Astrophysics Data System (ADS)

Han, Dongju

2018-05-01

Safe and efficient flight powered by an aircraft turbojet engine relies on the performance of the engine controller preventing compressor surge with robustness from noises or disturbances. This paper proposes the effective nonlinear controller associated with the nonlinear filter for the real turbojet engine with highly nonlinear dynamics. For the feasible controller study the nonlinearity of the engine dynamics was investigated by comparing the step responses from the linearized model with the original nonlinear dynamics. The fuzzy-based PID control logic is introduced to control the engine efficiently and FAUKF is applied for robustness from noises. The simulation results prove the effectiveness of FAUKF applied to the proposed controller such that the control performances are superior over the conventional controller and the filer performance using FAUKF indicates the satisfactory results such as clearing the defects by reducing the distortions without compressor surge, whereas the conventional UKF is not fully effective as occurring some distortions with compressor surge due to a process noise.

Effects of electromagnetic pulse exposure on gelatinase of blood-brain barrier in vitro.

PubMed

Zhou, Yan; Qiu, Lian-Bo; An, Guang-Zhou; Zhou, Jia-Xing; Du, Le; Ma, Ya-Hong; Guo, Guo-Zhen; Ding, Gui-Rong

2017-01-01

The biological effects of electromagnetic pulse (EMP) on the brain have been focused on for years. It was reported that gelatinase played an important role in maintaining brain function through regulating permeability in the blood-brain barrier (BBB). To investigate the effects of EMP on gelatinase of BBB, an in vitro BBB model was established using primary cultured rat brain microvascular endothelial cells (BMVEC), astrocytes and half-contact culture of these cells in a transwell chamber. Cultured supernatant and cells were collected at different time points after exposure to EMP (peak intensity 400 kV/m, rise time 10 ns, pulse width 350 ns, 0.5 pps and 200 pulses). Protein levels of cellular gelatinase MMP-2 and MMP-9, and endogenous inhibitor TIMP-1 and TIMP-2 were detected by Western blot. The activity of gelatinase in culture supernatant was detected by gelatin zymography. It was found that compared with the sham-exposed group, the protein level of MMP-2 was significantly increased at 6 h (p < 0.05), and the protein level of its endogenous inhibitor TIMP-2 did not change after EMP exposure. In addition, the protein levels of MMP-9 and its endogenous inhibitor TIMP-1 did not change after EMP exposure. Gelatin zymography results showed that the activity of MMP-2 in the inner pool and the outer pool of the transwell chamber was significantly increased at 6 h after EMP exposure compared with that of the sham group. These results suggested that EMP exposure could affect the expression and activity of MMP-2 in the BBB model.

[Effect of electromagnetic pulse irradiation on structure and function of Leydig cells in mice].

PubMed

Wang, Shui-Ming; Wang, De-Wen; Peng, Rui-Yun; Gao, Ya-Bing; Yang, Yi; Hu, Wen-Hua; Chen, Hao-Yu; Zhang, You-Ren; Gao, Yan

2003-08-01

To explore the effect of electromagnetic pulse (EMP) irradiation on structure and function of Leydig cells in mice. One hundred and fourteen male Kunming mice were randomly divided into irradiated and control group, the former radiated generally by 8 x 10(3) V/m, 2 x 10(4) V/m and 6 x 10(4) V/m EMP respectively five times within two minutes. Pathological changes of Leydig cells were observed by light and electron microscope. Serum testosterone (T), luteinizing hormone (LH) and estradiol (E2) were measured dynamically by radioimmunoassay at 6 h, 1 d, 3 d, 7 d, 14 d and 28 d after irradiation. Main pathological changes were edema and vacuolation, swelling of cytoplasmic mitochondria, reduce of lipid droplets, pale staining of most of lipid droplets, and partial or complete cavitation of lipid droplets in Leydig cells within 28 days after EMP radiation. Compared with normal controls, serum T decreased in all in different degrees within 28 days, and dropped significantly at 6 h-14 d, 6 h-7 d and 1 d-28 d after 8 x 10(3) V/m, 2 x 10(4) V/m and 6 x 10(4) V/m EMP irradiation(P < 0.05 or P < 0.01). EMP irradiation caused no significant changes in serum LH and E2. Leydig cells are among those that are the most susceptible to EMP irradiation. EMP irradiation may cause significant injury in structure and function of Leydig cells in mice, whose earlier and continuous effect is bound to affect sexual function and sperm production.

Differential diagnosis of acute rejection and chronic cyclosporine nephropathy after rat renal transplantation by detection of endothelial microparticles (EMP).

PubMed

Cui, Jiewei; Yang, Jing; Cao, Weike; Sun, Yi

2010-12-01

Endothelial microparticles (EMP) are small vesicles smaller than 1.0μm, released from endothelial cells (EC) during their activation and (or) apoptosis. The assay of the level of elevated EMP is a new approach to evaluate the dysfunction of endothelial cell. EMP can be classified into several types according to their membrane molecular, and the levels of various types of EMP may be different. As the most cost-effective immunodepressant, cyclosporine A (CsA) has been used widely in organ transplantation. But its dose is hard to control, under-medication may cause the acute rejection (AR) and overdose may cause chronic cyclosporine nephropathy (CCN). The cyclosporine A (CsA) caused CCN and the AR caused renal injury after renal transplantation are both vascular diseases related with endothelial dysfunction, and up to now, there is still no effective method to distinguish the two kinds of diseases. Owing to distinct pathogenesis of the two kinds of vascular diseases, the level of each type of EMP originated from vascular endothelial cells may be different. We hypothesize that maybe we can distinguish them by detecting the different levels of some types of EMP which is also related with vascular disease, and we propose to prove our hypothesis through animal experiment. If our hypothesis is proved, it will be more helpful for clinicians to adjust the dose of CsA promptly according to the differential diagnosis of the two kinds of diseases. Copyright © 2010 Elsevier Ltd. All rights reserved.

The Intriguing Tsunami of 19 March 2017 in the Persian Gulf

NASA Astrophysics Data System (ADS)

Mansouri, R.; Salaree, A.; Okal, E.

2017-12-01

On 19 March 2017, a surge from the Persian Gulf inundated significant parts of the Port of Dayyer on the southern coastlines of Iran. It, reportedly, capsized about 300 boats, caused $10 million of damage and resulted in one death and five people missing. Although a significant storm had preceded the surge, there were indications of calm weather during the event in some of the remaining footage which could play a critical role in reconstructing the tsunami by studying it as a meteorological phenomenon. Here, we are reporting the data from a field survey in the affected area which revealed a concentration of inundation along a 30 km stretch of coastline with run-ups reaching 3 m at 4 km west of Dayyer. In the absence of any major seismic event at or around the occurrence of the tsunami, we consider landslides as well as atmospheric phenomena as potential sources for the event. We use simultaneous hydrodynamic dipoles as landslide scenarios (e.g. Synolakis et al., 2002) and simulate water amplitudes close to the coastlines using the MOST algorithm (e.g. Titov & Synolakis, 1995). We also use the method by Platzman (1958) to model the surge as a meteotsunami. Our simulations suggest that the Dayyer tsunami was caused by a local system of atmospheric pressure gradient, as our landslide models fail to reproduce high amplitudes as documented in our field survey.

CD144+ endothelial microparticles as a marker of endothelial injury in neonatal ABO blood group incompatibility.

PubMed

Awad, Hisham A E; Tantawy, Azza A G; El-Farrash, Rania A; Ismail, Eman A; Youssif, Noha M

2014-04-01

ABO antigens are expressed on the surfaces of red blood cells and the vascular endothelium. We studied circulating endothelial microparticles (EMP) in ABO haemolytic disease of the newborn (ABO HDN) as a marker of endothelial activation to test a hypothesis of possible endothelial injury in neonates with ABO HDN, and its relation with the occurrence and severity of haemolysis. Forty-five neonates with ABO HDN were compared with 20 neonates with Rhesus incompatibility (Rh HDN; haemolytic controls) and 20 healthy neonates with matched mother and infant blood groups (healthy controls). Laboratory investigations were done for markers of haemolysis and von Willebrand factor antigen (vWF Ag). EMP (CD144(+)) levels were measured before and after therapy (exchange transfusion and/or phototherapy). vWF Ag and pre-therapy EMP levels were higher in infants with ABO HDN or Rh HDN than in healthy controls, and were significantly higher in babies with ABO HDN than in those with Rh HDN (p<0.05). In ABO HDN, pre-therapy EMP levels were higher in patients with severe hyperbilirubinaemia than in those with mild and moderate disease or those with Rh HDN (p<0.001). Post-therapy EMP levels were lower than pre-therapy levels in both the ABO HDN and Rh HDN groups; however, the decline in EMP levels was particularly evident after exchange transfusion in ABO neonates with severe hyperbilirubinaemia (p<0.001). Multiple regression analysis revealed that the concentrations of haemoglobin, lactate dehydrogenase and indirect bilirubin were independently correlated with pre-therapy EMP levels in ABO HDN. Elevated EMP levels in ABO HDN may reflect an IgG-mediated endothelial injury parallel to the IgG-mediated erythrocyte destruction and could serve as a surrogate marker of vascular dysfunction and disease severity in neonates with this condition.

Electronic medication packaging devices and medication adherence: a systematic review.

PubMed

Checchi, Kyle D; Huybrechts, Krista F; Avorn, Jerry; Kesselheim, Aaron S

2014-09-24

Medication nonadherence, which has been estimated to affect 28% to 31% of US patients with hypertension, hyperlipidemia, and diabetes, may be improved by electronic medication packaging (EMP) devices (adherence-monitoring devices incorporated into the packaging of a prescription medication). To investigate whether EMP devices are associated with improved adherence and to identify and describe common features of EMP devices. Systematic review of peer-reviewed studies testing the effectiveness of EMP systems in the MEDLINE, EMBASE, PsycINFO, CINAHL, International Pharmaceutical Abstracts, and Sociological Abstracts databases from searches conducted to June 13, 2014, with extraction of associations between the interventions and adherence, as well as other key findings. Each study was assessed for bias using the Cochrane Handbook for Systematic Reviews of Interventions; features of EMP devices and interventions were qualitatively assessed. Thirty-seven studies (32 randomized and 5 nonrandomized) including 4326 patients met inclusion criteria (10 patient interface-only "simple" interventions and 29 "complex" interventions integrated into the health care system [2 qualified for both categories]). Overall, the effect estimates for differences in mean adherence ranged from a decrease of 2.9% to an increase of 34.0%, and the those for differences in the proportion of patients defined as adherent ranged from a decrease of 8.0% to an increase of 49.5%. We identified 5 common EMP characteristics: recorded dosing events and stored records of adherence, audiovisual reminders to cue dosing, digital displays, real-time monitoring, and feedback on adherence performance. Many varieties of EMP devices exist. However, data supporting their use are limited, with variability in the quality of studies testing EMP devices. Devices integrated into the care delivery system and designed to record dosing events are most frequently associated with improved adherence, compared with other devices. Higher-quality evidence is needed to determine the effect, if any, of these low-cost interventions on medication nonadherence and to identify their most useful components.

[Alteration of Microparticle Levels in Early Complications During Hematopoietic Stem Cell Transplantation].

PubMed

Zhou, Li-Li; Han, Yue; Zhu, Qian; Zhao, Shi-Xiang; Wang, Qian; Zhu, Ming-Qing; Dai, Lan; Shen, Wen-Hong; Wu, De-Pei

2015-12-01

To investigate the alteration of microparticles (MP) in the recipients following hematopoietic stem cell transplantation (HSCT) and its significance, and to search the early diagnostic indicators of thrombotic complications after transplantation. According to the occurrence of transplantation-associated complications, 94 allo-HSCT patients were divided into 4 groups: thrombotic group (VOD n = 7, TMA n = 2), acute graft-versus-host disease (aGVHD) group (n = 27), infection group (n = 41) and non-complication group (n = 17). Alterations of serum concentration of tissue factor positive microparticles (TF(+) MP) and endothelial microparticles (EMP) were analyzed by flow cytometry during the process of conditioning treatment and the early stage after transplantation. The relation of these 2 kinds of MP with complications was analysed. (1) The levels of TF(+) MP and EMP of patients undogoing allo-HSCT before conditioning treatment were obviously higher than those in normal controls, and showed some elevation during different times, but there was no significant statistical difference. Although the levels of TF(+) MP and EMP at the end of conditioning treatment were some higher than those before conditioning treatment, but there was no statistical difference between them. (2)The levels of TF(+) MP and EMP in thrombotic group were obviously higher than those in aGVHD group and infection group (P < 0.05). (3)The levels of TF(+) MP and EMP in thrombotic group at different times were significant differences from those in other groups (P < 0.05), and the levels of TF(+) MP and EMP were no significant difference from those in non-complication group. The increase of the TF(+) MP and EMP levels may be associated with occurrence of thrombosis after transplantation, indicating occurrence of the thrombotic complications, like hepatic vein occulusive disease (HVOD). The dynamically monitoring levels of TF(+) MP and EMP contributes to early discovery of thrombotic complications.

Convalescent Plasmodium falciparum-specific seroreactivity does not correlate with paediatric malaria severity or Plasmodium antigen exposure.

PubMed

Kessler, Anne; Campo, Joseph J; Harawa, Visopo; Mandala, Wilson L; Rogerson, Stephen J; Mowrey, Wenzhu B; Seydel, Karl B; Kim, Kami

2018-04-25

Antibody immunity is thought to be essential to prevent severe Plasmodium falciparum infection, but the exact correlates of protection are unknown. Over time, children in endemic areas acquire non-sterile immunity to malaria that correlates with development of antibodies to merozoite invasion proteins and parasite proteins expressed on the surface of infected erythrocytes. A 1000 feature P. falciparum 3D7 protein microarray was used to compare P. falciparum-specific seroreactivity during acute infection and 30 days after infection in 23 children with uncomplicated malaria (UM) and 25 children with retinopathy-positive cerebral malaria (CM). All children had broad P. falciparum antibody reactivity during acute disease. IgM reactivity decreased and IgG reactivity increased in convalescence. Antibody reactivity to CIDR domains of "virulent" PfEMP1 proteins was low with robust reactivity to the highly conserved, intracellular ATS domain of PfEMP1 in both groups. Although children with UM and CM differed markedly in parasite burden and PfEMP1 exposure during acute disease, neither acute nor convalescent PfEMP1 seroreactivity differed between groups. Greater seroprevalence to a conserved Group A-associated ICAM binding extracellular domain was observed relative to linked extracellular CIDRα1 domains in both case groups. Pooled immune IgG from Malawian adults revealed greater reactivity to PfEMP1 than observed in children. Children with uncomplicated and cerebral malaria have similar breadth and magnitude of P. falciparum antibody reactivity. The utility of protein microarrays to measure serological recognition of polymorphic PfEMP1 antigens needs to be studied further, but the study findings support the hypothesis that conserved domains of PfEMP1 are more prominent targets of cross reactive antibodies than variable domains in children with symptomatic malaria. Protein microarrays represent an additional tool to identify cross-reactive Plasmodium antigens including PfEMP1 domains that can be investigated as strain-transcendent vaccine candidates.

High glucose derived endothelial microparticles increase active caspase-3 and reduce microRNA-Let-7a expression in endothelial cells.

PubMed

Bammert, Tyler D; Hijmans, Jamie G; Reiakvam, Whitney R; Levy, Ma'ayan V; Brewster, Lillian M; Goldthwaite, Zoe A; Greiner, Jared J; Stockelman, Kelly A; DeSouza, Christopher A

2017-11-18

The experimental aim of this study was to determine the effects of high glucose-induced endothelial microparticles (EMPs) on endothelial cell susceptibility to apoptosis. Human umbilical vein endothelial cells (HUVECs) were cultured (3rd passage) and plated in 6-well plates at a density of 5.0 × 10 5  cells/condition. Cells were incubated with media containing 25 mM d-glucose (concentration representing a diabetic glycemic state) or 5 mM d-glucose (normoglycemic condition) for 48 h to generate EMPs. EMP identification (CD144 + expression) and concentration was determined by flow cytometry. HUVECs (3 × 10 6  cells/condition) were treated with EMPs generated from either the normal or high glucose conditions for 24 h. Intracellular concentration of active caspase-3 was determined by enzyme immunoassay. Cellular expression of miR-Let7a, an anti-apoptotic microRNA, was determined by RT-PCR using the ΔΔCT normalized to RNU6. High glucose-derived EMPs significantly increased both basal (1.5 ± 0.1 vs 1.0 ± 0.1 ng/mL) and staurosporine-stimulated (2.2 ± 0.2 vs 1.4 ± 0.1 ng/mL) active caspase-3 compared with normal glucose EMPs. Additionally, the expression of miR-Let-7a was markedly reduced (∼140%) by high glucose EMPs (0.43 ± 0.17 fold vs control). These results demonstrate that hyperglycemic-induced EMPs increase endothelial cell active caspase-3. This apoptotic effect may be mediated, at least in part, by a reduction in miR-Let-7a expression. Copyright © 2017 Elsevier Inc. All rights reserved.

Fluid-structure interaction analysis and lifetime estimation of a natural gas pipeline centrifugal compressor under near-choke and near-surge conditions

NASA Astrophysics Data System (ADS)

Ju, Yaping; Liu, Hui; Yao, Ziyun; Xing, Peng; Zhang, Chuhua

2015-11-01

Up to present, there have been no studies concerning the application of fluid-structure interaction (FSI) analysis to the lifetime estimation of multi-stage centrifugal compressors under dangerous unsteady aerodynamic excitations. In this paper, computational fluid dynamics (CFD) simulations of a three-stage natural gas pipeline centrifugal compressor are performed under near-choke and near-surge conditions, and the unsteady aerodynamic pressure acting on impeller blades are obtained. Then computational structural dynamics (CSD) analysis is conducted through a one-way coupling FSI model to predict alternating stresses in impeller blades. Finally, the compressor lifetime is estimated using the nominal stress approach. The FSI results show that the impellers of latter stages suffer larger fluctuation stresses but smaller mean stresses than those at preceding stages under near-choke and near-surge conditions. The most dangerous position in the compressor is found to be located near the leading edge of the last-stage impeller blade. Compressor lifetime estimation shows that the investigated compressor can run up to 102.7 h under the near-choke condition and 200.2 h under the near-surge condition. This study is expected to provide a scientific guidance for the operation safety of natural gas pipeline centrifugal compressors.

Assessing the Risk of Catastrophic Cyber Attack: Lessons from the Electromagnetic Pulse Commission

DTIC Science & Technology

2015-04-15

need to address whether it would do so? Fourth, the question of consequences must include not only the direct effects of any attack but also potential...inform cyber consequence assessments. Sixth, EMP effects are difficult to characterize but ultimately are knowable at the device level. The effects ...that the approach the EMP Commission used to assess the likelihood and consequences of EMP attacks could provide useful lessons for analysts grappling

The eSurge-Venice project: altimeter and scatterometer satellite data to improve the storm surge forecasting in the city of Venice

NASA Astrophysics Data System (ADS)

Zecchetto, Stefano; De Biasio, Francesco; Umgiesser, Georg; Bajo, Marco; Vignudelli, Stefano; Papa, Alvise; Donlon, Craig; Bellafiore, Debora

2013-04-01

On the framework of the Data User Element (DUE) program, the European Space Agency is funding a project to use altimeter Total Water Level Envelope (TWLE) and scatterometer wind data to improve the storm surge forecasting in the Adriatic Sea and in the city of Venice. The project will: a) Select a number of Storm Surge Events occurred in the Venice lagoon in the period 1999-present day b) Provide the available satellite Earth Observation (EO) data related to the Storm Surge Events, mainly satellite winds and altimeter data, as well as all the available in-situ data and model forecasts c) Provide a demonstration Near Real Time service of EO data products and services in support of operational and experimental forecasting and warning services d) Run a number of re-analysis cases, both for historical and contemporary storm surge events, to demonstrate the usefulness of EO data The re-analysis experiments, based on hindcasts performed by the finite element 2-D oceanographic model SHYFEM (https://sites.google.com/site/shyfem/), will 1. use different forcing wind fields (calibrated and not calibrated with satellite wind data) 2. use Storm Surge Model initial conditions determined from altimeter TWLE data. The experience gained working with scatterometer and Numerical Weather Prediction (NWP) winds in the Adriatic Sea tells us that the bias NWP-Scatt wind is negative and spatially and temporally not uniform. In particular, a well established point is that the bias is higher close to coasts then offshore. Therefore, NWP wind speed calibration will be carried out on each single grid point in the Adriatic Sea domain over the period of a Storm Surge Event, taking into account of existing published methods. Point #2 considers two different methodologies to be used in re-analysis tests. One is based on the use of the TWLE values from altimeter data in the Storm Surge Model (SSM), applying data assimilation methodologies and trying to optimize the initial conditions of the simulation.The second possibility is an indirect exploitation of the TWLE data from altimeter in an ensemble-like framework, obtained by slight variations of the external forcing. In this case the wind data from NWP models will be weakly altered (shifted in phase), the drag coefficient will be modified, and the initial condition of the model slightly shifted in time to account for the uncertainty of these factors. This contribution will illustrate the geophysical context of work and outline the results.

Numerical simulation of rotating stall and surge alleviation in axial compressors

NASA Astrophysics Data System (ADS)

Niazi, Saeid

Axial compression systems are widely used in many aerodynamic applications. However, the operability of such systems is limited at low-mass flow rates by fluid dynamic instabilities. These instabilities lead the compressor to rotating stall or surge. In some instances, a combination of rotating stall and surge, called modified surge, has also been observed. Experimental and computational methods are two approaches for investigating these adverse aerodynamic phenomena. In this study, numerical investigations have been performed to study these phenomena, and to develop control strategies for alleviation of rotating stall and surge. A three-dimensional unsteady Navier-Stokes analysis capable of modeling multistage turbomachinery components has been developed. This method uses a finite volume approach that is third order accurate in space, and first or second order in time. The scheme is implicit in time, permitting the use of large time steps. A one-equation Spalart-Allmaras model is used to model the effects of turbulence. The analysis is cast in a very general form so that a variety of configurations---centrifugal compressors and multistage compressors---may be analyzed with minor modifications to the analysis. Calculations have been done both at design and off-design conditions for an axial compressor tested at NASA Glenn Research Center. At off-design conditions the calculations show that the tip leakage flow becomes strong, and its interaction with the tip shock leads to compressor rotating stall and modified surge. Both global variations to the mass flow rate, associated with surge, and azimuthal variations in flow conditions indicative of rotating stall, were observed. It is demonstrated that these adverse phenomena may be eliminated, and stable operation restored, by the use of bleed valves located on the diffuser walls. Two types of controls were examined: open-loop and closed-loop. In the open-loop case mass is removed at a fixed, preset rate from the diffuser. In the closed-loop case, the rate of bleed is linked to pressure fluctuations upstream of the compressor face. The bleed valve is activated when the amplitude of pressure fluctuations sensed by the probes exceeds a certain range. Calculations show that both types of bleeding eliminate both rotating stall and modified surge, and suppress the precursor disturbances upstream of the compressor face. It is observed that smaller amounts of compressed air need to be removed with the closed-loop control, as compared to open-loop control.

Surge-like Oscillations above Sunspot Light Bridges Driven by Magnetoacoustic Shocks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Jingwen; Tian, Hui; He, Jiansen

2017-03-20

High-resolution observations of the solar chromosphere and transition region often reveal surge-like oscillatory activities above sunspot light bridges (LBs). These oscillations are often interpreted as intermittent plasma jets produced by quasi-periodic magnetic reconnection. We have analyzed the oscillations above an LB in a sunspot using data taken by the Interface Region Imaging Spectrograph . The chromospheric 2796 Å images show surge-like activities above the entire LB at any time, forming an oscillating wall. Within the wall we often see that the core of the Mg ii k 2796.35 Å line first experiences a large blueshift, and then gradually decreases tomore » zero shift before increasing to a redshift of comparable magnitude. Such a behavior suggests that the oscillations are highly nonlinear and likely related to shocks. In the 1400 Å passband, which samples emission mainly from the Si iv ion, the most prominent feature is a bright oscillatory front ahead of the surges. We find a positive correlation between the acceleration and maximum velocity of the moving front, which is consistent with numerical simulations of upward propagating slow-mode shock waves. The Si iv 1402.77 Å line profile is generally enhanced and broadened in the bright front, which might be caused by turbulence generated through compression or by the shocks. These results, together with the fact that the oscillation period stays almost unchanged over a long duration, lead us to propose that the surge-like oscillations above LBs are caused by shocked p-mode waves leaked from the underlying photosphere.« less

Gel shift analysis of the empA promoter region in Vibrio anguillarum

PubMed Central

Denkin, Steven M; Sekaric, Pedja; Nelson, David R

2004-01-01

Background The induction of metalloprotease encoded by empA in Vibrio anguillarum occurs at high cell density in salmon intestinal mucus. Previously we have shown that there are significant differences in empA expression in two strains of V. anguillarum, M93Sm and NB10. It is hypothesized that differences in empA regulation are due to differences in binding of regulatory elements. Results Two strains of V. anguillarum, M93Sm and NB10, were examined and compared for the presence of DNA regulatory proteins that bind to and control the empA promoter region. Gel mobility shift assays, using a digoxigenin (DIG)-labeled oligomer containing a lux box-like element and the promoter for empA, were done to demonstrate the presence of a DNA-binding protein. Protein extracts from NB10 cells incubated in Luria Bertani broth + 2% NaCl (LB20), nine salts solution + 200 μg/ml mucus (NSSM), 3M (marine minimal medium), or NSS resulted in a gel mobility shift. No gel mobility shift was seen when protein extracts from either LB20- or NSSM-grown M93Sm cells were mixed with the DIG-labeled empA oligomer. The azocasein assay detected protease activity in all incubation conditions for NB10 culture supernatants. In contrast, protease activity was detected in M93Sm culture supernatants only when incubated in NSSM. Since the luxR homologue in V. anguillarum, vanT, has been cloned, sequenced, and shown to be required for protease activity, we wanted to determine if vanT mutants of NB10 exhibit the same gel shift observed in the wild-type. Site-directed mutagenesis was used to create vanT mutants in V. anguillarum M93Sm and NB10 to test whether VanT is involved with the gel mobility shift. Both vanT mutants, M02 and NB02, did not produce protease activity in any conditions. However, protein extracts from NB02 incubated in each condition still exhibited a gel shift when mixed with the DIG-labeled empA oligomer. Conclusions The data demonstrate that protein extracts of V. anguillarum NB10 cells contain a protein that binds to a 50 bp oligomer containing the empA promoter-lux box-like region. NB10 cells express empA during stationary phase in all growth conditions. The DNA binding protein is not present in M93Sm extracts. M93Sm cells express protease activity only when incubated at high cell density in fish gastrointestinal mucus. The gel shift observed with NB10 cells is not due to VanT binding. The data also suggest that the DNA binding protein is responsible for the less restrictive expression of empA in NB10 compared to M93Sm. PMID:15516264

Development of the Fully Adaptive Storm Tide (FAST) Model for hurricane induced storm surges and associated inundation

NASA Astrophysics Data System (ADS)

Teng, Y. C.; Kelly, D.; Li, Y.; Zhang, K.

2016-02-01

A new state-of-the-art model (the Fully Adaptive Storm Tide model, FAST) for the prediction of storm surges over complex landscapes is presented. The FAST model is based on the conservation form of the full non-linear depth-averaged long wave equations. The equations are solved via an explicit finite volume scheme with interfacial fluxes being computed via Osher's approximate Riemann solver. Geometric source terms are treated in a high order manner that is well-balanced. The numerical solution technique has been chosen to enable the accurate simulation of wetting and drying over complex topography. Another important feature of the FAST model is the use of a simple underlying Cartesian mesh with tree-based static and dynamic adaptive mesh refinement (AMR). This permits the simulation of unsteady flows over varying landscapes (including localized features such as canals) by locally increasing (or relaxing) grid resolution in a dynamic fashion. The use of (dynamic) AMR lowers the computational cost of the storm surge model whilst retaining high resolution (and thus accuracy) where and when it is required. In additional, the FAST model has been designed to execute in a parallel computational environment with localized time-stepping. The FAST model has already been carefully verified against a series of benchmark type problems (Kelly et al. 2015). Here we present two simulations of the storm tide due to Hurricane Ike(2008) and Hurricane Sandy (2012). The model incorporates high resolution LIDAR data for the major portion of the New York City. Results compare favorably with water elevations measured by NOAA tidal gauges, by mobile sensors deployed and high water marks collected by the USGS.

Field Research and Parametric Analysis in a Medical-Surgical Unit.

PubMed

Nanda, Upali; Pati, Sipra; Nejati, Adeleh

2015-01-01

To study the workplace in a medical-surgical (med-surg) unit and to identify suboptimal environmental conditions that can be improved in the current unit and avoided in future design, through rapidly deployed field research and timely simulation. Literature emphasizes the importance of the healthcare workplace and the effect on patient outcomes. What is lacking are studies conducted on-site and used for immediate application in design to assess and improve workplace conditions. A rapidly deployed field research and simulation study was conducted in a 40-bed med-surg unit of a large healthcare system as part of the process of designing a new medical tower. Online surveys, systematic behavioral observations, semi-structured interviews, sound studies, and advanced spatial analysis through parametric modeling were conducted. The following created challenges for patient monitoring, care coordination, and management: (1) waste and variability in walking, (2) limited point-of-use access to supplies, (3) large distances traveled for minor tasks, and (4) low visibility and connectivity. The corridor is used as a workspace/communication hub. There is a distinct difference in beginning of day and night shift patterns and between walking "distance" and walking "sequence." There is a tendency for nurses to multitask, but a simulation exercise shows that for key tasks like medication delivery, multitasking may not always reduce walking distances. Co-location of medications, supplies, and nourishment; accommodation for work on wheels; and spatial and technological connectivity between care team and patients should be considered while designing a med-surg unit. Understanding the key activity sequences helps determine the proximity of spaces in relationship to patient rooms and each other. © The Author(s) 2015.

Unusual coexistence of extramedullary plasmacytoma and nasopharyngeal carcinoma in nasopharynx.

PubMed

Du, Ri-Chang; Li, Hai-Nan; Huang, Wei; Tian, Xiao-Ying; Li, Zhi

2015-09-17

Nasopharyngeal carcinoma (NPC) is an EBV-associated malignant tumor of nasopharynx. As extremely rare condition, the second primary cancer of nasopharynx can occur in NPC patients synchronously or subsequently. Extramedullary plasmacytoma (EMP) is a rare tumor and commonly originates in the head and neck region. However, there is no report to describe a collision tumor of NPC and EMP occurring in the same nasopharyngeal mass. We report here an unusual case of synchronous coexistence of NPC and EMP occurring in the nasopharynx of an old male patient. A 63-year-old male patient presented with a 3-month history of right-sided nasal obstruction and recently intermittent epistaxis without enlargement of cervical lymph nodes. The solitary mass of nasopharynx was found by radiological and nasopharyngeal examination. Histologically, the mass contained two separated portions and displayed typically histological features of NPC and EMP, respectively. In EMP portion, the tumor was composed of monomorphic plasmacytoid-appearing cells with immuno-positive to CD79a, CD138, CD38, MUM-1 and CD56, but lack immunoreactivity to pan-CK (AE1/AE3), CD20, CD21 and EBERs. In NPC portion, the tumor cells formed irregular-shaped islands with diffusely immuno-positive to pan-CK (AE1/AE3), EMA and EBERs, but lack expressions of lymphoplasmacytic markers. A diagnosis of simultaneous occurrence of EMP and NPC in nasopharynx was made. There was no evidence of tumor recurrence or metastasis 18-month follow-up after radiotherapy. To our knowledge, it may be the first case of coexistence of EMP and NPC synchronously. In addition, the histological differential diagnosis and relevant potential mechanism of this unusual collision tumor were also discussed.

A case–control study of mesothelioma in Minnesota iron ore (taconite) miners

PubMed Central

Lambert, Christine S; Alexander, Bruce H; Ramachandran, Gurumurthy; MacLehose, Richard F; Nelson, Heather H; Ryan, Andrew D; Mandel, Jeffrey H

2018-01-01

Objectives An excess of mesothelioma has been observed in iron ore miners in Northeastern Minnesota. Mining and processing of taconite iron ore generate exposures that include elongate mineral particles (EMPs) of amphibole and non-amphibole origin. We conducted a nested case–control study of mesothelioma in a cohort of 68 737 iron ore miners (haematite and taconite ore miners) to evaluate the association between mesothelioma, employment and EMP exposures from taconite mining. Methods Mesothelioma cases (N=80) were identified through the Minnesota Cancer Surveillance System (MCSS) and death certificates. Four controls of similar age were selected for each case with 315 controls ultimately eligible for inclusion. Mesothelioma risk was evaluated by estimating rate ratios and 95% CIs with conditional logistic regression in relation to duration of taconite industry employment and cumulative EMP exposure [(EMP/cc)×years], defined by the National Institute for Occupational Safety and Health (NIOSH) 7400 method. Models were adjusted for employment in haematite mining and potential exposure to commercial asbestos products used in the industry. Results All mesothelioma cases were male and 57 of the cases had work experience in the taconite industry. Mesothelioma was associated with the number of years employed in the taconite industry (RR=1.03, 95% CI 1.00 to 1.06) and cumulative EMP exposure (RR=1.10, 95% CI 0.97 to –1.24). No association was observed with employment in haematite mining. Conclusions These results support an association between mesothelioma and employment duration and possibly EMP exposure in taconite mining and processing. The type of EMP was not determined. The potential role of commercial asbestos cannot be entirely ruled out. PMID:26655961

Current Status of Infection Prevention and Control Programs for Emergency Medical Personnel in the Republic of Korea.

PubMed

Oh, Hyang Soon; Uhm, Dong Choon

2015-11-01

Emergency medical personnel (EMPs) are pre-hospital emergency responders who are at risk of exposure to infections and may also serve as a source for the transmission of infections. However, few studies of infection control have specifically addressed EMPs in the Republic of Korea (hereafter Korea). The goal of this study was to assess the current status of infection prevention and control programs (IPCPs) for EMPs in Korea. A cross-sectional survey was conducted to quantitatively assess the resources and activities of IPCPs. A total of 907 EMPs in five metropolitan cities completed a structured questionnaire from September 2014 to January 2015. The data were analyzed using descriptive statistics, multi-response analysis, and the chi-square test. The mean age of the participants was 34.8±15.1 years. IPCPs were found to have weaknesses with regard to the following resources: the assignment of infection control personnel (ICP) (79.5%), hand hygiene resources such as waterless antiseptics (79.3%), the use of paper towels (38.9%), personal protective equipment such as face shields (46.9%), and safety containers for sharps and a separated space for the disposal of infectious waste (10.1%). Likewise, the following activities were found to be inadequately incorporated into the workflow of EMPs: education about infection control (77.5%), post-exposure management (35.9%), and the decontamination of items and spaces after use (88.4%). ICP were found to have a significant effect on the resources and activities of IPCPs (p<0.001). The resources and activities of IPCPs were found to be significantly different among the five cities (p<0.001). IPCPs for EMPs showed some limitations in their resources and activities. IPCPs should be actively supported, and specific IPCP activities for EMPs should be developed.

THE SYNTHETIC-OVERSAMPLING METHOD: USING PHOTOMETRIC COLORS TO DISCOVER EXTREMELY METAL-POOR STARS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, A. A., E-mail: amiller@astro.caltech.edu

2015-09-20

Extremely metal-poor (EMP) stars ([Fe/H] ≤ −3.0 dex) provide a unique window into understanding the first generation of stars and early chemical enrichment of the universe. EMP stars are exceptionally rare, however, and the relatively small number of confirmed discoveries limits our ability to exploit these near-field probes of the first ∼500 Myr after the Big Bang. Here, a new method to photometrically estimate [Fe/H] from only broadband photometric colors is presented. I show that the method, which utilizes machine-learning algorithms and a training set of ∼170,000 stars with spectroscopically measured [Fe/H], produces a typical scatter of ∼0.29 dex. Thismore » performance is similar to what is achievable via low-resolution spectroscopy, and outperforms other photometric techniques, while also being more general. I further show that a slight alteration to the model, wherein synthetic EMP stars are added to the training set, yields the robust identification of EMP candidates. In particular, this synthetic-oversampling method recovers ∼20% of the EMP stars in the training set, at a precision of ∼0.05. Furthermore, ∼65% of the false positives from the model are very metal-poor stars ([Fe/H] ≤ −2.0 dex). The synthetic-oversampling method is biased toward the discovery of warm (∼F-type) stars, a consequence of the targeting bias from the Sloan Digital Sky Survey/Sloan Extension for Galactic Understanding survey. This EMP selection method represents a significant improvement over alternative broadband optical selection techniques. The models are applied to >12 million stars, with an expected yield of ∼600 new EMP stars, which promises to open new avenues for exploring the early universe.« less

Increased Circulating Endothelial Apoptotic Microparticle to Endothelial Progenitor Cell Ratio Is Associated with Subsequent Decline in Glomerular Filtration Rate in Hypertensive Patients

PubMed Central

Hsu, Chien-Yi; Huang, Po-Hsun; Chiang, Chia-Hung; Leu, Hsin-Bang; Huang, Chin-Chou; Chen, Jaw-Wen; Lin, Shing-Jong

2013-01-01

Background Recent research indicates hypertensive patients with microalbuminuria have decreased endothelial progenitor cells (EPCs) and increased levels of endothelial apoptotic microparticles (EMP). However, whether these changes are related to a subsequent decline in glomerular filtration rate (GFR) remains unclear. Methods and Results We enrolled totally 100 hypertensive out-patients with eGFR ≥30 mL/min/1.73 m2. The mean annual rate of GFR decline (△GFR/y) was −1.49±3.26 mL/min/1.73 m2 per year during the follow-up period (34±6 months). Flow cytometry was used to assess circulating EPC (CD34+/KDR+) and EMP levels (CD31+/annexin V+) in peripheral blood. The △GFR/y was correlated with the EMP to EPC ratio (r = −0.465, p<0.001), microalbuminuria (r = −0.329, p = 0.001), and the Framingham risk score (r = −0.245, p = 0.013). When we divided the patients into 4 groups according to the EMP to EPC ratio, there was an association between the EMP to EPC ratio and the ΔGFR/y (mean ΔGFR/y: 0.08±3.04 vs. −0.50±2.84 vs. −1.25±2.49 vs. −4.42±2.82, p<0.001). Multivariate analysis indicated that increased EMP to EPC ratio is an independent predictor of ΔeGFR/y. Conclusions An increased circulating EMP to EPC ratio is associated with subsequent decline in GFR in hypertensive patients, which suggests endothelial damage with reduced vascular repair capacity may contribute to further deterioration of renal function in patients with hypertension. PMID:23874701

A case-control study of mesothelioma in Minnesota iron ore (taconite) miners.

PubMed

Lambert, Christine S; Alexander, Bruce H; Ramachandran, Gurumurthy; MacLehose, Richard F; Nelson, Heather H; Ryan, Andrew D; Mandel, Jeffrey H

2016-02-01

An excess of mesothelioma has been observed in iron ore miners in Northeastern Minnesota. Mining and processing of taconite iron ore generate exposures that include elongate mineral particles (EMPs) of amphibole and non-amphibole origin. We conducted a nested case-control study of mesothelioma in a cohort of 68,737 iron ore miners (haematite and taconite ore miners) to evaluate the association between mesothelioma, employment and EMP exposures from taconite mining. Mesothelioma cases (N=80) were identified through the Minnesota Cancer Surveillance System (MCSS) and death certificates. Four controls of similar age were selected for each case with 315 controls ultimately eligible for inclusion. Mesothelioma risk was evaluated by estimating rate ratios and 95% CIs with conditional logistic regression in relation to duration of taconite industry employment and cumulative EMP exposure [(EMP/cc)×years], defined by the National Institute for Occupational Safety and Health (NIOSH) 7400 method. Models were adjusted for employment in haematite mining and potential exposure to commercial asbestos products used in the industry. All mesothelioma cases were male and 57 of the cases had work experience in the taconite industry. Mesothelioma was associated with the number of years employed in the taconite industry (RR=1.03, 95% CI 1.00 to 1.06) and cumulative EMP exposure (RR=1.10, 95% CI 0.97 to -1.24). No association was observed with employment in haematite mining. These results support an association between mesothelioma and employment duration and possibly EMP exposure in taconite mining and processing. The type of EMP was not determined. The potential role of commercial asbestos cannot be entirely ruled out. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

Picosecond Dynamics of Excitonic Magnetic Polarons in Colloidal Diffusion-Doped Cd(1-x)Mn(x)Se Quantum Dots.

PubMed

Nelson, Heidi D; Bradshaw, Liam R; Barrows, Charles J; Vlaskin, Vladimir A; Gamelin, Daniel R

2015-11-24

Spontaneous magnetization is observed at zero magnetic field in photoexcited colloidal Cd(1-x)Mn(x)Se (x = 0.13) quantum dots (QDs) prepared by diffusion doping, reflecting strong Mn(2+)-exciton exchange coupling. The picosecond dynamics of this phenomenon, known as an excitonic magnetic polaron (EMP), are examined using a combination of time-resolved photoluminescence, magneto-photoluminescence, and Faraday rotation (TRFR) spectroscopies, in conjunction with continuous-wave absorption, magnetic circular dichroism (MCD), and magnetic circularly polarized photoluminescence (MCPL) spectroscopies. The data indicate that EMPs form with random magnetization orientations at zero external field, but their formation can be directed by an external magnetic field. After formation, however, external magnetic fields are unable to reorient the EMPs within the luminescence lifetime, implicating anisotropy in the EMP potential-energy surfaces. TRFR measurements in a transverse magnetic field reveal rapid (<5 ps) spin transfer from excitons to Mn(2+) followed by coherent EMP precession at the Mn(2+) Larmor frequency for over a nanosecond. A dynamical TRFR phase inversion is observed during EMP formation attributed to the large shifts in excitonic absorption energies during spontaneous magnetization. Partial optical orientation of the EMPs by resonant circularly polarized photoexcitation is also demonstrated. Collectively, these results highlight the extraordinary physical properties of colloidal diffusion-doped Cd(1-x)Mn(x)Se QDs that result from their unique combination of strong quantum confinement, large Mn(2+) concentrations, and relatively narrow size distributions. The insights gained from these measurements advance our understanding of spin dynamics and magnetic exchange in colloidal doped semiconductor nanostructures, with potential ramifications for future spin-based information technologies.

Retrospective survey of the efficacy of mandatory implementation of the Essential Medicine Policy in the primary healthcare setting in China: failure to promote the rational use of antibiotics in clinics.

PubMed

Xiao, Yonghong; Wang, Jin; Shen, Ping; Zheng, Beiwen; Zheng, Yingdong; Li, Lanjuan

2016-10-01

The objective of this study was to understand the impact of implementation of the Essential Medicine Policy (EMP) on the rational use of antibiotics in primary medical institutions in China. A retrospective survey was conducted in 39 primary medical institutions to compare the efficacy of EMP in rational antibiotic use. All institutions completed the survey 1 year before and 1 year after implementation of the EMP. In particular, antibiotic use and its rationality were closely examined. The institutions mainly dealt with common diseases, especially non-infectious chronic diseases. Antibiotic usage was very inappropriate both before and after EMP implementation. Before and after EMP implementation, respectively, the median outpatient cost was US$6.34 and US$5.05, 52.50% (2005/3819) and 53.41% (1865/3492) of the outpatient prescriptions contained antibiotics, and 76.23% (1132/1485) and 78.83% (1106/1403) of inpatients were administered antibiotics. In addition, 98.38% (425/432) and 97.52% (512/525) of surgical inpatients were administered antibiotics, respectively, and 80.76% (638/790) and 75.19% (503/669) of patients with a cold were prescribed antibiotics, respectively. The most commonly used antibiotics were broad-spectrum and injectable agents, including cephalosporins, fluoroquinolones and penicillins. This profile showed little change following implementation of the EMP. In conclusion, inappropriate antibiotic use is a serious problem in primary medical institutions in China. Whilst enforcing the EMP reduced the cost of medical services, it had little effect on promoting the rational use of antibiotics. Copyright © 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

Endothelial microparticles: Sophisticated vesicles modulating vascular function

PubMed Central

Curtis, Anne M; Edelberg, Jay; Jonas, Rebecca; Rogers, Wade T; Moore, Jonni S; Syed, Wajihuddin; Mohler, Emile R

2015-01-01

Endothelial microparticles (EMPs) belong to a family of extracellular vesicles that are dynamic, mobile, biological effectors capable of mediating vascular physiology and function. The release of EMPs can impart autocrine and paracrine effects on target cells through surface interaction, cellular fusion, and, possibly, the delivery of intra-vesicular cargo. A greater understanding of the formation, composition, and function of EMPs will broaden our understanding of endothelial communication and may expose new pathways amenable for therapeutic manipulation. PMID:23892447

Pathologic Mechanical Stress and Endotoxin Exposure Increases Lung Endothelial Microparticle Shedding

PubMed Central

Letsiou, Eleftheria; Sammani, Saad; Zhang, Wei; Zhou, Tong; Quijada, Hector; Moreno-Vinasco, Liliana; Dudek, Steven M.

2015-01-01

Acute lung injury (ALI) results from infectious challenges and from pathologic lung distention produced by excessive tidal volume delivered during mechanical ventilation (ventilator-induced lung injury [VILI]) and is characterized by extensive alveolar and vascular dysfunction. Identification of novel ALI therapies is hampered by the lack of effective ALI/VILI biomarkers. We explored endothelial cell (EC)-derived microparticles (EMPs) (0.1–1 μm) as potentially important markers and potential mediators of lung vascular injury in preclinical models of ALI and VILI. We characterized EMPs (annexin V and CD31 immunoreactivity) produced from human lung ECs exposed to physiologic or pathologic mechanical stress (5 or 18% cyclic stretch [CS]) or to endotoxin (LPS). EC exposure to 18% CS or to LPS resulted in increased EMP shedding compared with static cells (∼ 4-fold and ∼ 2.5-fold increases, respectively). Proteomic analysis revealed unique 18% CS–derived (n = 10) and LPS-derived EMP proteins (n = 43). VILI-challenged mice (40 ml/kg, 4 h) exhibited increased plasma and bronchoalveolar lavage CD62E (E-selectin)-positive MPs compared with control mice. Finally, mice receiving intratracheal instillation of 18% CS–derived EMPs displayed significant lung inflammation and injury. These findings indicate that ALI/VILI-producing stimuli induce significant shedding of distinct EMP populations that may serve as potential ALI biomarkers and contribute to the severity of lung injury. PMID:25029266

The role of radiation therapy in the treatment of solitary plasmacytomas.

PubMed

Mayr, N A; Wen, B C; Hussey, D H; Burns, C P; Staples, J J; Doornbos, J F; Vigliotti, A P

1990-04-01

Between 1960 and 1985, 30 patients with solitary plasmacytomas were treated with radiotherapy at the University of Iowa: 13 patients with extramedullary plasmacytomas (EMP) and 17 with solitary plasmacytomas of bone (SPB). The local control rates were 92% for patients with EMP and 88% for those with SPB. Two of nine patients (22%) with EMP treated to the primary tumor only developed regional lymph node metastasis, indicating the need for elective irradiation of this area. The most common pattern of failure in both groups was progression to multiple myeloma. This occurred in 23% of the patients with EMP and 53% of those with SPB. The time course of progression to multiple myeloma differed for the two groups. All of those who progressed to multiple myeloma in the EMP group did so within 2 years, whereas a significant number of those in the SPB group progressed more than 5 years after initial therapy. None of five patients who received adjuvant chemotherapy in the SPB group progressed to multiple myeloma, compared to 75% (9/12) of the patients who did not receive chemotherapy.

Study on the Shielding Effectiveness of an Arc Thermal Metal Spraying Method against an Electromagnetic Pulse

PubMed Central

Lee, Han-Seung; Choe, Hong-Bok; Baek, In-Young

2017-01-01

An electromagnetic pulse (EMP) explodes in real-time and causes critical damage within a short period to not only electric devices, but also to national infrastructures. In terms of EMP shielding rooms, metal plate has been used due to its excellent shielding effectiveness (SE). However, it has difficulties in manufacturing, as the fabrication of welded parts of metal plates and the cost of construction are non-economical. The objective of this study is to examine the applicability of the arc thermal metal spraying (ATMS) method as a new EMP shielding method to replace metal plate. The experimental parameters, metal types (Cu, Zn-Al), and coating thickness (100–700 μm) used for the ATMS method were considered. As an experiment, a SE test against an EMP in the range of 103 to 1010 Hz was conducted. Results showed that the ATMS coating with Zn-Al had similar shielding performance in comparison with metal plate. In conclusion, the ATMS method is judged to have a high possibility of actual application as a new EMP shielding material. PMID:28976931

Study on the Shielding Effectiveness of an Arc Thermal Metal Spraying Method against an Electromagnetic Pulse.

PubMed

Lee, Han-Seung; Choe, Hong-Bok; Baek, In-Young; Singh, Jitendra Kumar; Ismail, Mohamed A

2017-10-04

An electromagnetic pulse (EMP) explodes in real-time and causes critical damage within a short period to not only electric devices, but also to national infrastructures. In terms of EMP shielding rooms, metal plate has been used due to its excellent shielding effectiveness (SE). However, it has difficulties in manufacturing, as the fabrication of welded parts of metal plates and the cost of construction are non-economical. The objective of this study is to examine the applicability of the arc thermal metal spraying (ATMS) method as a new EMP shielding method to replace metal plate. The experimental parameters, metal types (Cu, Zn-Al), and coating thickness (100-700 μm) used for the ATMS method were considered. As an experiment, a SE test against an EMP in the range of 103 to 1010 Hz was conducted. Results showed that the ATMS coating with Zn-Al had similar shielding performance in comparison with metal plate. In conclusion, the ATMS method is judged to have a high possibility of actual application as a new EMP shielding material.

Electromagnetic Test-Facility characterization: an identification approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zicker, J.E.; Candy, J.V.

The response of an object subjected to high energy, transient electromagnetic (EM) fields sometimes called electromagnetic pulses (EMP), is an important issue in the survivability of electronic systems (e.g., aircraft), especially when the field has been generated by a high altitude nuclear burst. The characterization of transient response information is a matter of national concern. In this report we discuss techniques to: (1) improve signal processing at a test facility; and (2) parameterize a particular object response. First, we discuss the application of identification-based signal processing techniques to improve signal levels at the Lawrence Livermore National Laboratory (LLNL) EM Transientmore » Test Facility. We identify models of test equipment and then use these models to deconvolve the input/output sequences for the object under test. A parametric model of the object is identified from this data. The model can be used to extrapolate the response to these threat level EMP. Also discussed is the development of a facility simulator (EMSIM) useful for experimental design and calibration and a deconvolution algorithm (DECONV) useful for removing probe effects from the measured data.« less

Flooding Mitigation of seawalls and river embankments to storm surges in the coastal areas of Guangdong Province, China

NASA Astrophysics Data System (ADS)

Wang, Xianwei; Wang, Xina

2017-04-01

The coastal areas of Guangdong Province, China are susceptible to the destructions of tropical cyclones and storm surges. The projected global warming, coastal subsidence and sea level rise together will bring about greater flooding risk to these areas. The seawall and river embankment have played a significant role in mitigating and preventing the coastal low-land areas from the impairment of storm surges flooding and wave runup. However, few risk assessment studies in this region consider the existence of seawall and river embankment and often overestimate the risk and potential economic loss and population affected due to storm surge flooding. This study utilizes a hydraulic model to simulate the overtop flooding and compare those without seawall and river embankment using several specific tropic storm events and extreme events of tropic storm surges in different return periods of 2, 10, 20, 50, 100, 200 and 500 years. Most seawalls are 4 or 5 meters plus another meter of wave levee above the local mean sea level. The river embankments are usually 4 or 5 meter higher than the local mean sea level as well and decrease from the outer estuary to the inner riverine. The modeling results considering seawall and river embankments and from real storm surges are in agreement with on-site survey and observations, while those without infusing seawall and river embankments overestimate the inundation condition and economic loss. Modeling results demonstrate that seawall and river embankment greatly reduce the flooding risk and prevent the low-land area from inundation for most tropic storm events, e.g., for extreme events less than 20 to 50 years, in the coastal areas of Guangdong Province, China. However, the seawall and river embankment may also cause catastrophic disasters once there is an engineering failure of seawalls and river embankment, especially once encountering with an extreme typhoon event, e.g., the 1969 super typhoon Viola in Shantou China and the 2005 hurricane Katrina in New Orleans, USA.

Proceedings of the Conference on the Design of Experiments in Army Research, Development, and Testing (33rd)

DTIC Science & Technology

1988-05-01

Evaluation Directorate (ARMTE) was tasked to conduct a "side- by-side" comparison of EMPS vs . DATMs and to conduct a human factors evaluation of the EMPS...performance ("side-by-side") comparison of EMPS vs . DATMs and to conduct a human factors evaluation. The performance evaluation was based on the speed... independent targets over time. To acquire data for this research, the BRL conducted a statistically designed exper- iment, the Firepower Control Experiment

47 CFR 215.0 - Purpose and authority.

Code of Federal Regulations, 2010 CFR

2010-10-01

... POINT FOR ELECTROMAGNETIC PULSE (EMP) INFORMATION § 215.0 Purpose and authority. The purpose of this part is to designate a focal point within the Federal Government for electromagnetic pulse (EMP...

47 CFR 215.0 - Purpose and authority.

Code of Federal Regulations, 2013 CFR

2013-10-01

... POINT FOR ELECTROMAGNETIC PULSE (EMP) INFORMATION § 215.0 Purpose and authority. The purpose of this part is to designate a focal point within the Federal Government for electromagnetic pulse (EMP...

47 CFR 215.0 - Purpose and authority.

Code of Federal Regulations, 2011 CFR

2011-10-01

... POINT FOR ELECTROMAGNETIC PULSE (EMP) INFORMATION § 215.0 Purpose and authority. The purpose of this part is to designate a focal point within the Federal Government for electromagnetic pulse (EMP...

47 CFR 215.0 - Purpose and authority.

Code of Federal Regulations, 2014 CFR

2014-10-01

... POINT FOR ELECTROMAGNETIC PULSE (EMP) INFORMATION § 215.0 Purpose and authority. The purpose of this part is to designate a focal point within the Federal Government for electromagnetic pulse (EMP...

47 CFR 215.0 - Purpose and authority.

Code of Federal Regulations, 2012 CFR

2012-10-01

... POINT FOR ELECTROMAGNETIC PULSE (EMP) INFORMATION § 215.0 Purpose and authority. The purpose of this part is to designate a focal point within the Federal Government for electromagnetic pulse (EMP...

Impact of Sea Level Rise on Storm Surge and Inundation in the Northern Gulf of Mexico

NASA Astrophysics Data System (ADS)

Veeramony, J.

2016-12-01

Assessing the impact of climate change on surge and inundation due to tropical cyclones is important for coastal adaptation as well as mitigation efforts. Changes in global climate increase vulnerability of coastal environments to the threat posed by severe storms in a number of ways. Both the intensity of future storms as well as the return periods of more severe storms are expected to increase signficantly. Increasing mean sea levels lead to more areas being inundated due to storm surge and bring the threat of inundation further inland. Rainfall associated with severe storms are also expected to increase substantially, which will add to the intensity of inland flooding and coastal inundation. In this study, we will examine the effects of sea level rise and increasing rainfall intensity using Hurricane Ike as the baseline. The Delft3D modeling system will be set up in nested mode, with the outermost nest covering the Gulf of Mexico. The system will be run in a coupled mode, modeling both waves and the hydrodynamics. The baseline simulation will use the atmospheric forcing which consists of the NOAA H*Wind (Powell et all 1998) for the core hurricane characteristics blended with reanalyzed background winds to create a smooth wind field. The rainfall estimates are obtained from TRMM. From this baseline, a set of simulations will be performed to show the impact of sea level rise and increased rainfall activity on flooding and inundation along theTexas-Lousiana coast.

Extreme Statistics of Storm Surges in the Baltic Sea

NASA Astrophysics Data System (ADS)

Kulikov, E. A.; Medvedev, I. P.

2017-11-01

Statistical analysis of the extreme values of the Baltic Sea level has been performed for a series of observations for 15-125 years at 13 tide gauge stations. It is shown that the empirical relation between value of extreme sea level rises or ebbs (caused by storm events) and its return period in the Baltic Sea can be well approximated by the Gumbel probability distribution. The maximum values of extreme floods/ebbs of the 100-year recurrence were observed in the Gulf of Finland and the Gulf of Riga. The two longest data series, observed in Stockholm and Vyborg over 125 years, have shown a significant deviation from the Gumbel distribution for the rarest events. Statistical analysis of the hourly sea level data series reveals some asymmetry in the variability of the Baltic Sea level. The probability of rises proved higher than that of ebbs. As for the magnitude of the 100-year recurrence surge, it considerably exceeded the magnitude of ebbs almost everywhere. This asymmetry effect can be attributed to the influence of low atmospheric pressure during storms. A statistical study of extreme values has also been applied to sea level series for Narva over the period of 1994-2000, which were simulated by the ROMS numerical model. Comparisons of the "simulated" and "observed" extreme sea level distributions show that the model reproduces quite satisfactorily extreme floods of "moderate" magnitude; however, it underestimates sea level changes for the most powerful storm surges.

A simulation model for projecting changes in salinity concentrations and species dominance in the coastal margin habitats of the Everglades

USGS Publications Warehouse

Teh, S.Y.; DeAngelis, D.L.; Sternberg, L.D.S.L.; Miralles-Wilhelm, F. R.; Smith, T.J.; Koh, H. L.

2008-01-01

Sharp boundaries typically separate the salinity tolerant mangroves from the salinity intolerant hardwood hammock species, which occupy the similar geographical areas of southern Florida. Evidence of strong feedback between tree community-type and the salinity of the unsaturated (vadose) zone of the soil suggests that a severe disturbance that significantly tilts the salinity in the vadose zone might cause a shift from one vegetation type to the other. In this study, a model based upon the feedback dynamics between vegetation and salinity of the vadose zone of the soil was used to take account of storm surge events to investigate the mechanisms that by which this large-scale disturbance could affect the spatial pattern of hardwood hammocks and mangroves. Model simulation results indicated that a heavy storm surge that completely saturated the vadose zone at 30 ppt for 1 day could lead to a regime shift in which there is domination by mangroves of areas previously dominated by hardwood hammocks. Lighter storm surges that saturated the vadose zone at less than 7 ppt did not cause vegetation shifts. Investigations of model sensitivity analysis indicated that the thickness of the vadose zone, coupled with precipitation, influenced the residence time of high salinity in the vadose zone and therefore determined the rate of mangrove domination. The model was developed for a southern Florida coastal ecosystem, but its applicability may be much broader. ?? 2008 Elsevier B.V. All rights reserved.

Microsimulation of Financial Impact of Demand Surge on Hospitals: The H1N1 Influenza Pandemic of Fall 2009

PubMed Central

Braithwaite, Sabina; Friedman, Bernard; Mutter, Ryan; Handrigan, Michael

2013-01-01

Objective Microsimulation was used to assess the financial impact on hospitals of a surge in influenza admissions in advance of the H1N1 pandemic in the fall of 2009. The goal was to estimate net income and losses (nationally, and by hospital type) of a response of filling unused hospital bed capacity proportionately and postponing elective admissions (a “passive” supply response). Methods Epidemiologic assumptions were combined with assumptions from other literature (e.g., staff absenteeism, profitability by payer class), Census data on age groups by region, and baseline hospital utilization data. Hospital discharge records were available from the Healthcare Cost and Utilization Project Nationwide Inpatient Sample (NIS). Hospital bed capacity and staffing were measured with the American Hospital Association's (AHA) Annual Survey. Results Nationwide, in a scenario of relatively severe epidemiologic assumptions, we estimated aggregate net income of $119 million for about 1 million additional influenza-related admissions, and a net loss of $37 million for 52,000 postponed elective admissions. Implications Aggregate and distributional results did not suggest that a policy of promising additional financial compensation to hospitals in anticipation of the surge in flu cases was necessary. The analysis identified needs for better information of several types to improve simulations of hospital behavior and impacts during demand surges. PMID:23398540

Electromagnetic Pulse (EMP) survey of the Louisiana State Emergency Operating Center, Baton Rouge, Louisiana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Crutcher, R.I.; Buchanan, M.E.; Jones, R.W.

1989-08-01

The purpose of this report is to develop an engineering design package to protect the federal Emergency Management Agency (FEMA) National Radio System (FNARS) facilities from the effects of high-altitude electromagnetic pulses (HEMP). This report refers to the Louisiana State Emergency Operating Center (EOC) in Baton Rouge, Louisiana. This report addresses electromagnetic pulse (EMP) effects only, and disregards any condition in which radiation effects may be a factor. It has been established that, except for the source region of a surface burst, EMP effects of high-altitude bursts are more severe than comparable detonations in either air or surface regions. Anymore » system hardened to withstand the more extreme EMP environment will survive the less severe conditions. The threatening environment will therefore be limited to HEMP situations. 76 figs., 2 tabs.« less

The use of commercial off-the-shelf equipment and its impact on C3 (Command, Control and Communications EMP (electromagnetic pulse) survivability

NASA Astrophysics Data System (ADS)

Hoke, Melvin D., Jr.

1988-04-01

The President's Blue Ribbon Commission on Defense Management has recommended that the military should make greater use of off-the-shelf components, systems, and services (also know as Non-Developmental Items or NDI). This recommendation, if blindly implemented, could have an adverse impact on the survivability of critical command, control, and communications assets. The study evaluates how the electromagnetic pulse (EMP) survivability of a strategic, time-urgent, fixed, ground-based, Command, Control, and Communications (C3) facility would be impacted by the use of off-the-shelf C3 equipments. The study concludes that to have a quantifiable measure of confidence in the facility's EMP survivability, the piece of off-the-shelf equipment under consideration will first have to be subjected to some degree of EMP testing.

Nuclear pulse. III - Playing a wild card

NASA Astrophysics Data System (ADS)

Broad, W. J.

1981-06-01

Implications of the phenomenon of electromagnetic pulse (EMP), a high-voltage by-product of nuclear explosions in space which could render useless unprotected communications equipment and power grids over a wide area, for the feasibility of conducting a limited nuclear war by the United States are discussed. Arguments on the one hand that the effects of EMP demand direct investigation and should be protected against by the hardening of U.S. military communications are summarized and contrasted with those on the other hand which assert that the presence of EMP, as well as other exotic nuclear effects, would, despite any attempts at hardening, make it impossible to maintain the precision of command and control necessary for a limited nuclear action against Soviet military targets. Uncertainties about Soviet intentions in regard to the use of EMP as a weapon are also pointed out.

TOPoS: chemical study of extremely metal-poor stars.

NASA Astrophysics Data System (ADS)

Caffau, E.; Sbordone, L.; Bonifacio, P.; Cayrel, R.; Christlieb, N.; Clark, P.; François, P.; Glover, S.; Klessen, R.; Koch, A.; Ludwig, H.-G.; Monaco, L.; Plez, B.; Spite, F.; Spite, M.; Steffen, M.; Zaggia, S.

The extremely metal-poor (EMP) stars hold in their atmospheres the fossil record of the chemical composition of the early phases of the Galactic evolution. The chemical analysis of such objects provides important constraints on these early phases. EMP stars are very rare objects; to dig them out, large amounts of data have to be processed. With an automatic procedure, we analysed objects with colours of Turn-Off stars from the Sloan Digital Sky Survey to select a sample of good candidate EMP stars. In the latest years, we observed a sample of these candidates with X-Shooter and UVES, and we have an ongoing ESO large programme to use these spectrographs to observe EMP stars. I will report here the results on metallicity and Strontium abundance. Based on observations obtained at ESO Paranal Observatory, programme 189.D-0165(A)

Extramedullary plasmacytoma in the carotid space: Expanding the differential diagnosis.

PubMed

Deshpande, Sneha Satish; Kane, Shubhada; Arya, Supreeta

2014-10-01

Plasma cell neoplasms have been classified into various types, with a range of clinical and radiological presentations. Extramedullary plasmacytoma (EMP) is a subset of plasma cell neoplasms which presents as an isolated non-osseous soft tissue mass. Though carotid space neoplasms are commonly encountered, EMP in the carotid space is rare and seldom considered in the initial differential diagnosis of a carotid space mass. These tumors can be treated by surgery or radiotherapy. On the other hand, the commonly encountered tumors in the carotid space are treated surgically. Also, it is mandatory to exclude multiple myeloma in the patients presenting with EMP. Hence, accurate and early diagnosis has therapeutic and prognostic implications. We report a rare case of EMP of the carotid space, describing the imaging features and the differential diagnoses with clues pointing to this rare entity.

A Search for Meteoroid Lunar Impact Generated Electromagnetic Pulses

NASA Astrophysics Data System (ADS)

Kesaraju, Saiveena; Mathews, John D.; Vierinen, Juha; Perillat, Phil; Meisel, David D.

2016-11-01

Lunar white light flashes associated with meteoroid impacts are now regularly observed using modest optical instrumentation. In this paper, we hypothesize that the developing, optically-dense hot ejecta cloud associated with these hypervelocity impacts also produce an associated complex plasma component that rapidly evolves resulting in a highly-transient electro magnetic pulse (EMP) in the VHF/UHF spectral region. Discovery of the characteristics and event frequency of impact EMPs would prove interesting to meteoroid flux and complex plasma physics studies especially if EMPs from the same event are detected from at least two locations on the Earth with relative delays appropriate to the propagation paths. We describe a prototype observational search, conducted in May 2014, for meteoroid lunar-impact EMPs that was conducted using simultaneous, overlapping-band, UHF radio observations at the Arecibo (AO; Puerto Rico) and Haystack (HO, Massachusetts, USA) Observatories. Monostatic/bistatic lunar radar imaging observations were also performed with HO transmitting and HO/AO receiving to confirm tracking, the net delay, and the pointing/timing ephemeris at both observatories. Signal analysis was performed using time-frequency signal processing techniques. Although, we did not conclusively identify EMP returns, this search detected possible EMPs and we have confirmed the search paradigm and established the sensitivity of the AO-HO system in detecting the hypothesized events. We have also characterized the difficult radio-frequency interference environment surrounding these UHF observations. We discuss the wide range of terrestrial-origin, Moon-bounce signals that were observed which additionally validate the observational technique. Further observations are contemplated.

EPOR-Based Purification and Analysis of Erythropoietin Mimetic Peptides from Human Urine by Cys-Specific Cleavage and LC/MS/MS

NASA Astrophysics Data System (ADS)

Vogel, Matthias; Thomas, Andreas; Schänzer, Wilhelm; Thevis, Mario

2015-09-01

The development of a new class of erythropoietin mimetic agents (EMA) for treating anemic conditions has been initiated with the discovery of oligopeptides capable of dimerizing the erythropoietin (EPO) receptor and thus stimulating erythropoiesis. The most promising amino acid sequences have been mounted on various different polymeric structures or carrier molecules to obtain highly active EPO-like drugs exhibiting beneficial and desirable pharmacokinetic profiles. Concomitant with creating new therapeutic options, erythropoietin mimetic peptide (EMP)-based drug candidates represent means to artificially enhance endurance performance and necessitate coverage by sports drug testing methods. Therefore, the aim of the present study was to develop a strategy for the comprehensive detection of EMPs in doping controls, which can be used complementary to existing protocols. Three model EMPs were used to provide proof-of-concept data. Following EPO receptor-facilitated purification of target analytes from human urine, the common presence of the cysteine-flanked core structure of EMPs was exploited to generate diagnostic peptides with the aid of a nonenzymatic cleavage procedure. Sensitive detection was accomplished by targeted-SIM/data-dependent MS2 analysis. Method characterization was conducted for the EMP-based drug peginesatide concerning specificity, linearity, precision, recovery, stability, ion suppression/enhancement, and limit of detection (LOD, 0.25 ng/mL). Additionally, first data for the identification of the erythropoietin mimetic peptides EMP1 and BB68 were generated, demonstrating the multi-analyte testing capability of the presented approach.

NUCLEOSYNTHESIS IN HIGH-ENTROPY HOT BUBBLES OF SUPERNOVAE AND ABUNDANCE PATTERNS OF EXTREMELY METAL-POOR STARS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Izutani, Natsuko; Umeda, Hideyuki, E-mail: izutani@astron.s.u-tokyo.ac.j, E-mail: umeda@astron.s.u-tokyo.ac.j

2010-09-01

There have been suggestions that the abundance of extremely metal-poor (EMP) stars can be reproduced by hypernovae (HNe), not by normal supernovae (SNe). However, recently it was also suggested that if the innermost neutron-rich or proton-rich matter is ejected, the abundance patterns of ejected matter are changed, and normal SNe may also reproduce the observations of EMP stars. In this Letter, we calculate explosive nucleosynthesis with various Y {sub e} and entropy, and investigate whether normal SNe with this innermost matter, which we call the 'hot-bubble' component, can reproduce the abundance of EMP stars. We find that neutron-rich (Y {submore » e} = 0.45-0.49) and proton-rich (Y {sub e} = 0.51-0.55) matter can increase Zn/Fe and Co/Fe ratios as observed, but tend to overproduce other Fe-peak elements. In addition, we find that if slightly proton-rich matter with 0.50 {<=} Y {sub e} < 0.501 with s/k {sub b} {approx} 15-40 is ejected as much as {approx}0.06 M {sub sun}, even normal SNe can reproduce the abundance of EMP stars, though it requires fine-tuning of Y {sub e}. On the other hand, HNe can more easily reproduce the observations of EMP stars without fine-tuning. Our results imply that HNe are the most likely origin of the abundance pattern of EMP stars.« less

Losses Assessment of Crops due to Typhoon Disaster in China Coastal Areas —— A Case Study of Zhanjiang City, Guangdong Province

NASA Astrophysics Data System (ADS)

Mo, W.; Fang, W.

2015-12-01

Vulnerability which quantifies the loss ratio under different hazard intensity is an important feature of the natural disaster system and has important significance to natural disaster risk assessment. Agriculture is an outdoor industry with high risk of meteorological disasters. The strong winds, heavy rain and storm surge are main typhoon hazard factors to crops. To provide a quantitative research method for the loss evaluation of crops due to typhoon disaster we first revised two vulnerability curves for crops under comprehensive intensity of typhoon based on the simulated hazard data and loss data related to historical typhoon events landing on China from 1949 to 2014；and then established a storm surge vulnerability matrix of crops regarding Zhanjiang City of Guangdong Province as the study area ; finally, we put forward three storm surge fragility curves for crops representing different states of loss. The results can effectively describe the typhoon vulnerability for crops in China coastal areas so as to provide the input to post-disaster loss assessments and catastrophe modeling applications.

Power Management in Regenerative Life Support Systems

NASA Technical Reports Server (NTRS)

Crawford, Sekou; Pawlowski, Christopher; Finn, Cory; Mead, Susan C. (Technical Monitor)

1999-01-01

Effective management of power can reduce the cost of launch and operation of regenerative life support systems. Variations in power may be quite severe and may manifest as surges or spikes, While the power plant may have some ability to deal with these variations, with batteries for example, over-capacity is expensive and does nothing to address the fundamental issue of excessive demand. Because the power unit must be sized to accommodate the largest demand, avoiding power spikes has the potential to reduce the required size of the power plant while at the same time increasing the dependability of the system. Scheduling of processors can help to reduce potential power spikes. However, not all power-consuming equipment is easily scheduled. Therefore, active power management is needed to further decrease the risk of surges or spikes. We investigate the use of a hierarchical scheme to actively manage power for a model of a regenerative life support system. Local level controllers individually determine subsystem power usage. A higher level controller monitors overall system power and detects surges or spikes. When a surge condition is detected, the higher level controller conducts an 'auction' and describes subsystem power usage to re-allocate power. The result is an overall reduction in total power during a power surge. The auction involves each subsystem making a 'bid' to buy or sell power based on local needs. However, this re-allocation cannot come at the expense of life support function. To this end, participation in the auction is restricted to those processes meeting certain tolerance constraints. These tolerances represent acceptable limits within which system processes can be operated. We present a simulation model and discuss some of our results.

Possibility Of Generating Significant Storm Surge On The Western Seaboard Of Metro Manila, Philippines

NASA Astrophysics Data System (ADS)

Suarez, J. K. B.; Santiago, J. T.; Tablazon, J. P.; Dasallas, L. L.; Goting, P. G.; Lagmay, A. M. A.

2016-12-01

The Philippines, located in the Northwestern Pacific Typhoon gateway to Asia, is considered one of the most susceptible to tropical cyclone related hazards. One of the most disastrous effects of tropical cyclones is storm surge. With Metro Manila being a coastal area and the most populous region in the country, with approximately 12.8 million people residing in it, it is of great interest to determine the possibility of generating significant level of storm surge in the country's capital. The necessity to determine the storm surge susceptibility was brought upon by the effect of Typhoon Haiyan on eastern Visayas in 2013, where more than 6,000 people died and resulted to about 2.86 billion dollars' worth of damages. To achieve the objectives, the actual tracks and wind speed of historical typhoon (JMA data since 1951) was mapped for the Philippines. The simulated wind speed map shows that the maximum winds are mostly experienced on the eastern side of the country; with a considerable decrease in wind intensity as the typhoon reaches the western seaboard due to land surface. The Haiyan-strength wind speed is then applied to the actual historical typhoon tracks to determine the hypothetical values of wind speed as a typhoon with Haiyan intensity reached Metro Manila. Results show that, if a typhoon with a Haiyan-like intensity is to traverse tracks like those of Rita 1978, Collen 1992, Sybil 1995, Bebinca 2000 and Xangsane 2000, there is a huge possibility of generating storm surge height of 3.9 to 5.6 m in the western seaboard of Metro Manila, even after considering the diminishing effect of surface friction.

78 FR 69405 - Enable Gas Transmission, LLC; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-19

..., by sell and transfer from Enable to Enable Midstream Partners, LP (EMP), certain facilities and... Leedey Purification Facility and to abandon by sale to EMP the following facilities in Oklahoma: (1) The...

7 CFR 1486.402 - What are ineligible contributions?

Code of Federal Regulations, 2013 CFR

2013-01-01

... Recipient or third party, e.g., free publicity; (10) The cost of developing any application/proposal for EMP... approval of an EMP-funded project. (b) The Deputy Administrator shall determine, at his or her discretion...

Efficiency at maximum power for an isothermal chemical engine with particle exchange at varying chemical potential

NASA Astrophysics Data System (ADS)

Koning, Jesper; Koga, Kenichiro; Indekeu, Joseph. O.

2017-02-01

We calculate the efficiency at maximum power (EMP) of an isothermal chemical cycle in which particle uptake occurs at a fixed chemical potential but particle release takes place at varying chemical potential. We obtain the EMP as a function of Δμ/ kT, where Δμ is the difference between the highest and lowest reservoir chemical potentials and T is the absolute temperature. In the linear response limit, Δμ ≪ kT, the EMP tends to the expected universal value 1/2.

Kinetics of B cell responses to Plasmodium falciparum erythrocyte membrane protein 1 in Ghanaian women naturally exposed to malaria parasites.

PubMed

Ampomah, Paulina; Stevenson, Liz; Ofori, Michael F; Barfod, Lea; Hviid, Lars

2014-06-01

Naturally acquired protective immunity to Plasmodium falciparum malaria takes years to develop. It relies mainly on Abs, particularly IgG specific for Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) proteins on the infected erythrocyte surface. It is only partially understood why acquisition of clinical protection takes years to develop, but it probably involves a range of immune-evasive parasite features, not least of which are PfEMP1 polymorphism and clonal variation. Parasite-induced subversion of immunological memory and expansion of "atypical" memory B cells may also contribute. In this first, to our knowledge, longitudinal study of its kind, we measured B cell subset composition, as well as PfEMP1-specific Ab levels and memory B cell frequencies, in Ghanaian women followed from early pregnancy up to 1 y after delivery. Cell phenotypes and Ag-specific B cell function were assessed three times during and after pregnancy. Levels of IgG specific for pregnancy-restricted, VAR2CSA-type PfEMP1 increased markedly during pregnancy and declined after delivery, whereas IgG levels specific for two PfEMP1 proteins not restricted to pregnancy did not. Changes in VAR2CSA-specific memory B cell frequencies showed typical primary memory induction among primigravidae and recall expansion among multigravidae, followed by contraction postpartum in all. No systematic changes in the frequencies of memory B cells specific for the two other PfEMP1 proteins were identified. The B cell subset analysis confirmed earlier reports of high atypical memory B cell frequencies among residents of P. falciparum-endemic areas, and indicated an additional effect of pregnancy. Our study provides new knowledge regarding immunity to P. falciparum malaria and underpins efforts to develop PfEMP1-based vaccines against this disease. Copyright © 2014 by The American Association of Immunologists, Inc.

Synthesis, crystal structures, quantum chemical studies and corrosion inhibition potentials of 4-(((4-ethylphenyl)imino)methyl)phenol and (E)-4-((naphthalen-2-ylimino) methyl) phenol Schiff bases

NASA Astrophysics Data System (ADS)

Elemike, Elias E.; Nwankwo, Henry U.; Onwudiwe, Damian C.; Hosten, Eric C.

2017-11-01

Two Schiff base ligands, 4-(((4-ethylphenyl)imino)methyl)phenol (4EMP) and (E)-4-((naphthalen-2-ylimino) methyl) phenol (4NMP) were synthesized by the reaction of 4-hydroxybenzaldehyde with 4-ethylaniline, 4EMP, or naphthalene-2-amine, 4NMP. The compounds were characterized using NMR (1H and 13C), Fourier transform infra-red (FTIR) and mass spectroscopic techniques. The proton NMR identified the OH peaks at 9.73 and 9.77 ppm for 4EMP and 4NMP respectively, while the 13C NMR showed the imine carbons at 172.57 ppm for 4EMP and at 160.89 ppm for 4NMP. The FTIR spectra showed characteristic peaks at 1605 cm-1 (4EMP) and 1600 cm-1 (4NMP) typical of the azomethine group, and the mass spectra results gave molecular ion peaks of 226.12 and 248.10 respectively. The structures of the compounds were further established by single crystal X-ray analysis. The corrosion inhibition potential of the compounds were studied on mild steel surface in a 1 M hydrochloric acid (HCl) solution, and was analysed using potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS). The results of the electrochemical methods showed that the studied molecules imparted high resistance in allowing flow of electrons across the metal-electrolyte platform and behaved as mixed type inhibitors with 4EMP showing better inhibition properties than 4NMP. Scanning electron microscopy (SEM) showed the formation of film on the mild steel surface. Quantum chemical calculations achieved by density functional theory (DFT) was further applied to explain the adsorption as well as inhibition abilities of the molecules on the mild steel surface. Thermodynamics studies showed that the two compounds obeyed the Langmuir isotherm with 4EMP conforming to chemisorption mechanism while 4NMP involved competitive physisorption and chemisorption mechanism.

Topical Treatment With an Agent Disruptive to P. acnes Biofilm Provides Positive Therapeutic Response: Results of a Randomized Clinical Trial.

PubMed

Bernhardt, Michael J; Myntti, Matthew F

2016-06-01

The traditional disease model of acne has been one of follicular plugging due to 'sticky epithelial cells' associated with increased sebum production with deep follicular anaerobic conditions favoring P. acnes- generated inflammation. P. acnes biofilms have been found more frequently in patients with acne than controls. Biofilms are genetically coded to create adhesion to the pilosebaceous unit followed by production of a mucopolysaccharide coating capable of binding to lipid surfaces. Traditional therapies for acne have involved mixtures of oral and topical antibiotics admixed with topical keratolytics and retinoids, which are aimed at traditional bacterial reduction as well as downregulating the inflammatory cascade. These approaches are limited by side effect and compliance/tolerability issues. As the P. acnes biofilm may, in fact, be the instigator of this process, we studied the use of a topical agent designed to reduce the P. acnes biofilm to see if reducing the biofilm would be therapeutically efficacious. We present data of a proprietary topical non-prescription agent with a novel pharmaco mechanism designed to attack the biofilm produced by P. acnes. Our data shows a decrease of inflammatory lesions by 44% and non-inflammatory lesions by 32% after 12 weeks and also provided for a meaningful improvement in the quality of life of the patients in the study. These improvements were achieved with a product that was not associated with burning, chafing, irritation, or erythema, which can be seen with topical treatments. It is apparent from this study that by addressing the biofilm which protects the P. acnes bacteria through the use of the Acne Gel, the incidence of acne symptoms can be greatly reduced, while having no negative impacts on the patients' skin (ClinicalTrials.gov registry number NCT02404285).

J Drugs Dermatol. 2016;15(6):677-683.

Cyclone Nargis survey in Myanmar's Ayeyarwady River delta

NASA Astrophysics Data System (ADS)

Fritz, H. M.; Blount, C.; Thwin, S.; Thu, M. K.; Chan, N.

2008-12-01

Tropical cyclone Nargis (Cat. 4) made landfall on May 2, 2008, causing the worst natural disaster in Myanmar's recorded history. Official death toll estimates exceed 130,000 fatalities making it the 7th deadliest cyclone ever recorded worldwide. Nargis took a rare nearly eastern track over the Bay of Bengal while developing sustained winds over 210 km/h with gusts up to 260 km/h hours prior to landfall in Myanmar at untypically low latitude near 16°N. It then proceeded northeast and approximately 12 hours later weakened to a Category 1 storm with sustained wind speeds of 130 km/h as it passed over Yangon. The first independent storm surge reconnaissance team was deployed to Myanmar from 9 to 23 August 2008. Cyclone Nargis struck low-lying coastal plains particularly vulnerable to storm surge flooding due to the lack of effective barriers. The team surveyed coastal and inland villages from Pyapon to Purian Point, encompassing the Bogale and Ayeyarwady River mouths. The survey by boat spanned more than 150 km parallel to the cyclone track between Pyapon and Pyinkhayan encompassing 20 hardest hit settlements such as Pyinsalu. More than 1m vertical erosion and 150 m land loss were measured at various coastal locations such as Aya. Massive deforestation of mangroves and land use were documented. Maximum storm surge elevations and overland flow depths were measured based on water marks on buildings, scars on trees, and rafted debris. The storm surge peaked in the landfall area south of Pyinkhayan and eastwards in Pyinsalu exceeding 5m. Storm waves more than 2m high were superimposed on the storm surge level in most areas according to eyewitnesses. Inundation distances reached beyond 50 km inland. Catastrophic peak fatality rates exceeded 80% in hardest hit villages with the majority being children and women. The high water marks and fatality rates significantly exceeded corresponding 2004 Indian Ocean tsunami values at every location. Eyewitnesses were interviewed to document the time history of the event, survival strategies, cyclone awareness and evacuation. At the end of the 3 month relief operations survivors were left drinking from rice paddies with contaminated wells and no source of safe drinking water besides rain water. Storm surge simulations can be benchmarked against the measured cyclone Nargis storm surge and inundation to perform vulnerability analysis. Unfortunately, the widely deforested, low lying and densely populated Ayeyarwady delta remains extremely vulnerable to future storm surge flooding or potential sea level rise.

Effect of azilsartan versus candesartan on morning blood pressure surges in Japanese patients with essential hypertension

PubMed Central

Kario, Kazuomi; Enya, Kazuaki; Sugiura, Kenkichi; Ikeda, Yoshinori

2014-01-01

Morning blood pressure (BP) surge is reported as a risk factor for cardiovascular events and end-organ damage independent of the 24-h BP level. Controlling morning BP surge is therefore important to help prevent onset of cardiovascular disease. We compared the efficacy of azilsartan and candesartan in controlling morning systolic BP (SBP) surges by analyzing relevant ambulatory BP monitoring data in patients with/without baseline BP surges. As part of a 16-week randomized, double-blind study of azilsartan (20–40 mg once daily) and candesartan (8–12 mg once daily) in Japanese patients with essential hypertension, an exploratory analysis was carried out using ambulatory BP monitoring at baseline and week 14. The effects of study drugs on morning BP surges, including sleep trough surge (early morning SBP minus the lowest night-time SBP) and prewaking surge (early morning SBP minus SBP before awakening), were evaluated. Patients with sleep trough surge of at least 35 mmHg were defined by the presence of a morning BP surge (the ‘surge group’). Sleep trough surge and prewaking surge data were available at both baseline and week 14 in 548 patients, 147 of whom (azilsartan 76; candesartan 71) had a baseline morning BP surge. In surge group patients, azilsartan significantly reduced both the sleep trough surge and the prewaking surge at week 14 compared with candesartan (least squares means of the between-group differences −5.8 mmHg, P=0.0395; and −5.7 mmHg, P=0.0228, respectively). Once-daily azilsartan improved sleep trough surge and prewaking surge to a greater extent than candesartan in Japanese patients with grade I–II essential hypertension. PMID:24710336

Effect of azilsartan versus candesartan on morning blood pressure surges in Japanese patients with essential hypertension.

PubMed

Rakugi, Hiromi; Kario, Kazuomi; Enya, Kazuaki; Sugiura, Kenkichi; Ikeda, Yoshinori

2014-06-01

Morning blood pressure (BP) surge is reported as a risk factor for cardiovascular events and end-organ damage independent of the 24-h BP level. Controlling morning BP surge is therefore important to help prevent onset of cardiovascular disease. We compared the efficacy of azilsartan and candesartan in controlling morning systolic BP (SBP) surges by analyzing relevant ambulatory BP monitoring data in patients with/without baseline BP surges. As part of a 16-week randomized, double-blind study of azilsartan (20-40 mg once daily) and candesartan (8-12 mg once daily) in Japanese patients with essential hypertension, an exploratory analysis was carried out using ambulatory BP monitoring at baseline and week 14. The effects of study drugs on morning BP surges, including sleep trough surge (early morning SBP minus the lowest night-time SBP) and prewaking surge (early morning SBP minus SBP before awakening), were evaluated. Patients with sleep trough surge of at least 35 mmHg were defined by the presence of a morning BP surge (the 'surge group'). Sleep trough surge and prewaking surge data were available at both baseline and week 14 in 548 patients, 147 of whom (azilsartan 76; candesartan 71) had a baseline morning BP surge. In surge group patients, azilsartan significantly reduced both the sleep trough surge and the prewaking surge at week 14 compared with candesartan (least squares means of the between-group differences -5.8 mmHg, P=0.0395; and -5.7 mmHg, P=0.0228, respectively). Once-daily azilsartan improved sleep trough surge and prewaking surge to a greater extent than candesartan in Japanese patients with grade I-II essential hypertension.

EMP Threats to US National Security: Congressional Responses

NASA Astrophysics Data System (ADS)

Huessy, Peter

2011-04-01

The US Congress is considering how best to respond to concerns that EMP is a real and present danger to US security. The threats come from a variety of areas: solar storms, non-nuclear EMP from man-made machines and devices; and nuclear EMP from a nuclear device exploded above CONUS or other critical areas important to the United States and its allies. Responses have to date included passage in the House of legislation to protect the electrical grid in the United States from such threats and hearings before the Homeland Security Committee. Additional efforts include examining missile defense responses, protection of the maritime domain, and hardening of US military and related civilian infrastructure. The House of Representatives has also examined what Europe, the European Union and NATO, both government and private industry, have done in these areas. Complicating matters are related issues of cyber-security and overall homeland security priorities.

EMP-induced BBB-disruption enhances drug delivery to glioma and increases treatment efficacy in rats.

PubMed

Li, Kangchu; Zhang, Keying; Xu, Shenglong; Wang, Xiaowu; Zhou, Yongchun; Zhou, Yan; Gao, Peng; Lin, Jiajin; Ding, Guirong; Guo, Guozhen

2018-01-01

Chemotherapy on gliomas is not satisfactorily efficient because the presence of blood-brain barriers (BBB) leads to inadequate exposure of tumor cells to administered drugs. In order to facilitate chemotherapeutics to penetrate BBB and increase the treatment efficacy of gliomas, electromagnetic pulse (EMP) was applied and the 1-(2-Chlorethyl)-cyclohexyl-nitrosourea (CCNU) lomustine concentration in tumor tissue, tumor size, tumor apoptosis, and side effects were measured in glioma-bearing rat model. The results showed that EMP exposure could enhance the delivery of CCNU to tumor tissue, facilitate tumor apoptosis, and inhibit tumor growth without obvious side effects. The data indicated that EMP-induced BBB disruption could enhance delivery of CCNU to glioblastoma multiforme and increase treatment efficacy in glioma-bearing rats. Bioelectromagnetics. 39:60-67, 2018. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Some factors influencing susceptibility of rainbow trout to the acute toxicity of an ethyl mercury phosphate formulation (Timsan)

USGS Publications Warehouse

Amend, Donald F.; Yasutake, William T.; Morgan, Reginald

1969-01-01

This study determined the influence of water temperature (55–68° F), dissolved oxygen (4–12 ppm), water hardness as CaCO3 (20–256 ppm), and chloride ions (to 2 mM) on the susceptibility of rainbow trout (Salmo gairdneri) to the acute toxicity of ethyl mercury phosphate (EMP). The fish were exposed for one hour to 0.125 ppm EMP, the active ingredient of Timsan, a commercial EMP formulation. The death rate because of the exposure to EMP increased with an increase in water temperature, a decrease in dissolved oxygen, and an increase in chloride ions; calcium appeared to have no effect. The effect of water temperature and dissolved oxygen was ascribed to changes in the respiration rate of the fish, and a chemical explanation is presented for the effect of chloride ions.

Impact of expression of EMP enzymes on glucose metabolism in Zymomonas mobilis.

PubMed

Chen, Rachel Ruizhen; Agrawal, Manoj; Mao, Zichao

2013-06-01

Zymomonas mobilis is the only known microorganism that utilizes the Entner-Doudoroff (ED) pathway anaerobically. In this work, we investigated whether the overexpression of a phosphofructokinase (PFK), the only missing Embden-Meyerhof-Parnas (EMP) pathway enzyme, could establish the pathway in this organism. Introduction of a pyrophosphate-dependent PFK, along with co-expression of homologous fructose-1,6-bisphosphate aldolase and triosephosphate isomerase, did not result in an EMP flux to any appreciable level. However, the metabolism of glucose was impacted significantly. Eight percent of glucose was metabolized to form a new metabolite, dihydroxyacetone. Reducing flux through the ED pathway by as much as 40 % through antisense of a key enzyme, ED aldolase, did not result in a fully functional EMP pathway, suggesting that the ED pathway, especially the lower arm, downstream from glyceraldehyde-3-phosphate, is very rigid, possibly due to redox balance.

A cross-country Exchange Market Pressure (EMP) dataset.

PubMed

Desai, Mohit; Patnaik, Ila; Felman, Joshua; Shah, Ajay

2017-06-01

The data presented in this article are related to the research article titled - "An exchange market pressure measure for cross country analysis" (Patnaik et al. [1]). In this article, we present the dataset for Exchange Market Pressure values (EMP) for 139 countries along with their conversion factors, ρ (rho). Exchange Market Pressure, expressed in percentage change in exchange rate, measures the change in exchange rate that would have taken place had the central bank not intervened. The conversion factor ρ can interpreted as the change in exchange rate associated with $1 billion of intervention. Estimates of conversion factor ρ allow us to calculate a monthly time series of EMP for 139 countries. Additionally, the dataset contains the 68% confidence interval (high and low values) for the point estimates of ρ 's. Using the standard errors of estimates of ρ 's, we obtain one sigma intervals around mean estimates of EMP values. These values are also reported in the dataset.

Effects of electromagnetic pulse on polydactyly of mouse fetuses.

PubMed

Yang, Ming-Juan; Liu, Jun-Ye; Wang, Ya-Feng; Lang, Hai-Yang; Miao, Xia; Zhang, Li-Yan; Zeng, Li-Hua; Guo, Guo-Zhen

2013-07-01

There is an increasing public concern regarding potential health impacts from electromagnetic radiation exposure. Embryonic development is sensitive to the external environment, and limb development is vital for life quality. To determine the effects of electromagnetic pulse (EMP) on polydactyly of mouse fetuses, pregnant mice were sham-exposed or exposed to EMP (400 kV/m with 400 pulses) from Days 7 to 10 of pregnancy (Day 0 = day of detection of vaginal plug). As a positive control, mice were treated with 5-bromodeoxyuridine on Days 9 and 10. On Days 11 or 18, the fetuses were isolated. Compared with the sham-exposed group, the group exposed to EMP had increased rates of polydactyly fetuses (5.1% vs. 0.6%, P < 0.05) and abnormal gene expression (22.2% vs. 2.8%, P < 0.05). Ectopic expression of Fgf4 was detected in the apical ectodermal ridge, whereas overexpression and ectopic expression of Shh were detected in the zone of polarizing activity of limbs in the EMP-exposed group and in the positive control group. However, expression of Gli3 decreased in mesenchyme cells in those two groups. The percentages of programmed cell death of limbs in EMP-exposed and positive control group were decreased (3.57% and 2.94%, respectively, P < 0.05, compared with 7.76% in sham-exposed group). In conclusion, polydactyly induced by EMP was accompanied by abnormal expression of the above-mentioned genes and decreased percentage of programmed cell death during limb development. Copyright © 2013 Elsevier Inc. All rights reserved.

The Severity of Plasmodium falciparum Infection Is Associated with Transcript Levels of var Genes Encoding Endothelial Protein C Receptor-Binding P. falciparum Erythrocyte Membrane Protein 1.

PubMed

Mkumbaye, Sixbert I; Wang, Christian W; Lyimo, Eric; Jespersen, Jakob S; Manjurano, Alphaxard; Mosha, Jacklin; Kavishe, Reginald A; Mwakalinga, Steven B; Minja, Daniel T R; Lusingu, John P; Theander, Thor G; Lavstsen, Thomas

2017-04-01

By attaching infected erythrocytes to the vascular lining, Plasmodium falciparum parasites leave blood circulation and avoid splenic clearance. This sequestration is central to pathogenesis. Severe malaria is associated with parasites expressing an antigenically distinct P. falciparum erythrocyte membrane protein 1 (PfEMP1) subset mediating binding to endothelial receptors. Previous studies indicate that PfEMP1 adhesins with so-called CIDRα1 domains capable of binding endothelial protein C receptor (EPCR) constitute the PfEMP1 subset associated with severe pediatric malaria. To analyze the relative importance of different subtypes of CIDRα1 domains, we compared Pf emp1 transcript levels in children with severe malaria (including 9 fatal and 114 surviving cases), children hospitalized with uncomplicated malaria ( n = 42), children with mild malaria not requiring hospitalization ( n = 10), and children with parasitemia and no ongoing fever ( n = 12). High levels of transcripts encoding EPCR-binding PfEMP1 were found in patients with symptomatic infections, and the abundance of these transcripts increased with disease severity. The compositions of CIDRα1 subtype transcripts varied markedly between patients, and none of the subtypes were dominant. Transcript-level analyses targeting other domain types indicated that subtypes of DBLβ or DBLζ domains might mediate binding phenomena that, in conjunction with EPCR binding, could contribute to pathogenesis. These observations strengthen the rationale for targeting the PfEMP1-EPCR interaction by vaccines and adjunctive therapies. Interventions should target EPCR binding of all CIDRα1 subtypes. Copyright © 2017 American Society for Microbiology.

Association between vascular access failure and microparticles in hemodialysis patients

PubMed Central

Ryu, Jung-Hwa; Lim, Su-Young; Ryu, Dong-Ryeol; Kang, Duk-Hee; Choi, Kyu Bok; Kim, Seung-Jung

2012-01-01

Background Vascular access failure, a major cause of morbidity in hemodialysis (HD) patients, occurs mainly at stenotic endothelium following an acute thrombotic event. Microparticles (MPs) are fragments derived from injured cell membrane and are closely associated with coagulation and vascular inflammatory responses. Methods We investigated the relationship between levels of circulating MPs and vascular access patency in HD patients. A total of 82 HD patients and 28 healthy patients were enrolled. We used flow cytometry to measure endothelial MPs (EMPs) identified by CD31+CD42− or CD51+ and platelet-derived MPs (PMPs) identified by CD31+CD42+ in plasma samples of participants. Vascular access patency was defined as an interval from the time of access formation to the time of first access stenosis in each patient. MP counts were compared according to access patent duration. Results The levels of EMP (both CD31+CD42− and CD51+) and CD31+CD42+PMP were significantly higher in patients than in healthy participants. Levels of CD31+CD42−EMP and CD31+CD42+PMP showed a positive correlation. In non-diabetic HD patients, CD31+CD42−EMPs and CD31+CD42+PMPs were more elevated in the shorter access survival group (access survival <1 year) than in the longer survival group (access survival ≥ 4 years). Conclusion Elevated circulating EMP or PMP counts are influenced by end-stage renal disease and increased levels of EMP and PMP may be associated with vascular access failure in HD patients. PMID:26889407

Ethanol Extract of Mylabris phalerata Inhibits M2 Polarization Induced by Recombinant IL-4 and IL-13 in Murine Macrophages

PubMed Central

Chung, Hwan-Suck; Lee, Bong-Seon

2017-01-01

Mylabris phalerata (MP) is an insect used in oriental herbal treatments for tumor, tinea infections, and stroke. Recent studies have shown that tumor-associated macrophages (TAM) have detrimental roles such as tumor progression, angiogenesis, and metastasis. Although TAM has phenotypes and characteristics in common with M2-polarized macrophages, M1 macrophages have tumor suppression and immune stimulation effects. Medicines polarizing macrophages to M1 have been suggested to have anticancer effects via the modulation of the tumor microenvironment. In this line, we screened oriental medicines to find M1 polarizing medicines in M2-polarized macrophages. Among approximately 400 types of oriental medicine, the ethanol extract of M. phalerata (EMP) was the most proficient in increasing TNF-α secretion in M2-polarized macrophages and TAM. Although EMP enhanced the levels of an M1 cytokine (TNF-α) and a marker (CD86), it significantly reduced the levels of an M2 marker (arginase-1) in M2-polarized macrophages. In addition, EMP-treated macrophages increased the levels of M1 markers (Inos and Tnf-α) and reduced those of the enhanced M2 markers (Fizz-1, Ym-1, and arginase-1). EMP-treated macrophages significantly reduced Lewis lung carcinoma cell migration in a transwell migration assay and inhibited EL4-luc2 lymphoma proliferation. In our mechanism study, EMP was found to inhibit STAT3 phosphorylation in M2-polarized macrophages. These results suggest that EMP is effective in treating TAM-mediated tumor progression and metastasis. PMID:28811825

Evasion of Immunity to Plasmodium falciparum: Rosettes of Blood Group A Impair Recognition of PfEMP1

PubMed Central

Moll, Kirsten; Palmkvist, Mia; Ch'ng, Junhong; Kiwuwa, Mpungu Steven; Wahlgren, Mats

2015-01-01

The ABO blood group antigens are expressed on erythrocytes but also on endothelial cells, platelets and serum proteins. Notably, the ABO blood group of a malaria patient determines the development of the disease given that blood group O reduces the probability to succumb in severe malaria, compared to individuals of groups A, B or AB. P. falciparum rosetting and sequestration are mediated by PfEMP1, RIFIN and STEVOR, expressed at the surface of the parasitized red blood cell (pRBC). Antibodies to these antigens consequently modify the course of a malaria infection by preventing sequestration and promoting phagocytosis of pRBC. Here we have studied rosetting P. falciparum and present evidence of an immune evasion mechanism not previously recognized. We find the accessibility of antibodies to PfEMP1 at the surface of the pRBC to be reduced when P. falciparum forms rosettes in blood group A RBC, as compared to group O RBC. The pRBC surrounds itself with tightly bound normal RBC that makes PfEMP1 inaccessible to antibodies and clearance by the immune system. Accordingly, pRBC of in vitro cloned P. falciparum devoid of ABO blood group dependent rosetting were equally well detected by anti-PfEMP1 antibodies, independent of the blood group utilized for their propagation. The pathogenic mechanisms underlying the severe forms of malaria may in patients of blood group A depend on the ability of the parasite to mask PfEMP1 from antibody recognition, in so doing evading immune clearance. PMID:26714011

Comparison of the 2008-2011 and 1993-1995 Surges of Bering Glacier, Alaska

NASA Astrophysics Data System (ADS)

Molnia, B. F.; Angeli, K.

2011-12-01

The 1993-1995 surge of Bering Glacier, Earth's largest surging temperate glacier, was intensively studied. A new surge, which began prior to March 9, 2009, was still active in early August 2011. As was the 1993-1995 surge, the current surge is being studied using multiple remote sensing and ground-based methodologies. The wealth of observations available of both surges permit comparisons to be drawn about similarities and differences regarding processes, timing, intensities, and related topics. For more than a year prior to each surge, the intensity of calving and the rate of terminus retreat in Tashalich Arm increased dramatically, approaching 4 m/d in late 2010. This was abruptly followed by a significant terminus advance. In the current surge, maximum advance rates exceeded 19 m/d between March 18 and May 10, 2011. Through July 20, maximum terminus advance approached 3.2 km with velocities above 8 m/d. Similar rates applied in the earlier surge. Each surge has resulted in a rapid and significant advance of the central Bering Lobe's terminus into Vitus Lake. The terminus advance results from the transfer of a substantial volume of ice from the Bagley Ice Valley into the expanding piedmont lobe. In both surges, conspicuous evidence of tens of meters of glacier surface lowering is visible on the south wall of Juniper Island. In the 1993-1995 surge, terminus advance between October 17, 1993 and May 16, 1994 was nearly 7.8 km, an average advance rate of more than 36 m/d. With the current surge, between January 8 and 14, 2011, the terminus advanced a maximum of 125 m, averaging nearly 21 m/d. By July 11, 2011, maximum velocities still approached 15 m per day, with maximum ice displacements of nearly 2 km and a maximum terminus advance of 1.7 km. In the 1993-1995 surge, the first evidence of surge activity was observed in April 1993, the development of a fractured ice bulge on the northwest side of the Grindle Hills. The surge front reached Bering's terminus at the end of August 1993 and left it heavily fractured. With the current surge, until July 2009, surface displacements were restricted to the area from west of, to northeast of the Grindle Hills. By November 18, 2010, the surge front reached Bering's terminus and left it more heavily fractured than in 1993. The current surge shows the same style and types of surface disruptions and deformations at the same locations as did the earlier surge. For example, in both surges, sinusoidal crevasses were first noted north of the Grindle Hills, while rifts were noted in the upper central piedmont lobe. The current surge has produced much more fracturing of the Medial Moraine Band than did the 1993-95 surge. Similarly, the extent of surface fracturing up-glacier from the piedmont lobe is significantly greater in the current surge. During the 1993-95 surge, surface expression of the surge extended about 45 km east of the western end of Juniper Island. In late July 2011, surge-related surface fractures extended nearly 90 km to the east. The Steller lobe of the Bering Glacier System has not been involved in either surge. Continued observations of the current surge, in the context of the 1993-95 surge, are providing significant insights into repeatable patterns of surging glacier behavior. Bering Glacier is an amazing natural laboratory at which to conduct these observations.

Tropical cyclone induced asymmetry of sea level surge and fall and its presentation in a storm surge model with parametric wind fields

NASA Astrophysics Data System (ADS)

Peng, Machuan; Xie, Lian; Pietrafesa, Leonard J.

The asymmetry of tropical cyclone induced maximum coastal sea level rise (positive surge) and fall (negative surge) is studied using a three-dimensional storm surge model. It is found that the negative surge induced by offshore winds is more sensitive to wind speed and direction changes than the positive surge by onshore winds. As a result, negative surge is inherently more difficult to forecast than positive surge since there is uncertainty in tropical storm wind forecasts. The asymmetry of negative and positive surge under parametric wind forcing is more apparent in shallow water regions. For tropical cyclones with fixed central pressure, the surge asymmetry increases with decreasing storm translation speed. For those with the same translation speed, a weaker tropical cyclone is expected to gain a higher AI (asymmetry index) value though its induced maximum surge and fall are smaller. With fixed RMW (radius of maximum wind), the relationship between central pressure and AI is heterogeneous and depends on the value of RMW. Tropical cyclone's wind inflow angle can also affect surge asymmetry. A set of idealized cases as well as two historic tropical cyclones are used to illustrate the surge asymmetry.

7 CFR 1486.100 - What is the Emerging Markets Program?

Code of Federal Regulations, 2012 CFR

2012-01-01

... What is the Emerging Markets Program? (a) The principal purpose of the EMP is to assist U.S. entities... training, business workshops, and similar undertakings. (b) The EMP may be used to support exports of U.S...

7 CFR 1486.100 - What is the Emerging Markets Program?

Code of Federal Regulations, 2014 CFR

2014-01-01

... What is the Emerging Markets Program? (a) The principal purpose of the EMP is to assist U.S. entities... training, business workshops, and similar undertakings. (b) The EMP may be used to support exports of U.S...

7 CFR 1486.100 - What is the Emerging Markets Program?

Code of Federal Regulations, 2013 CFR

2013-01-01

... What is the Emerging Markets Program? (a) The principal purpose of the EMP is to assist U.S. entities... training, business workshops, and similar undertakings. (b) The EMP may be used to support exports of U.S...

Final report on low-dose estramustine phosphate (EMP) monotherapy and very low-dose EMP therapy combined with LH-RH agonist for previously untreated advanced prostate cancer.

PubMed

Kitamura, T; Suzuki, M; Nishimatsu, H; Kurosaki, T; Enomoto, Y; Fukuhara, H; Kume, H; Takeuchi, T; Miao, L; Jiangang, H; Xiaoqiang, L

2010-01-01

In order to assess the efficacy and toxicity of oral estramustine phosphate (EMP) administration, low-dose EMP monotherapy (study 1) and very low-dose EMP therapy with luteinizing hormone-releasing hormone (LH-RH) agonist (study 2) were conducted in previously untreated prostate cancer and long-term outcomes were compared between the 2 study groups. Studies 1 and 2 were independently performed beginning in June 1999 and November 2001, respectively. Study 1 was composed of 87 patients including 85 assessable patients. All 108 patients recruited for study 2 were assessable. Low-dose EMP monotherapy (2 capsules/day or 280 mg/day) was used in study 1 and very low-dose EMP (1 capsule/day or 140 mg/day) combined with LH-RH agonist was adopted in study 2. Overall prostate specific antigen (PSA) -response rates in studies 1 and 2 were 92.3% and 94.2%, respectively, and overall toxicity rates were 54.1% and 38.9%, respectively. EMP discontinuation due to side effects was encountered more often in study 1 (45.9%) than in study 2 (27.8%). Among the adverse side effects gastrointestinal toxicity was most prevalent in both studies. One patient died of acute pulmonary embolism in study 1, but no one died in study 2. There were 6 cancer deaths in the gastrointestinal tract in study 1 but only 2 cancer deaths in study 2. Our data indicate that the overall PSA response rate was comparable between both studies. However, rates in overall toxicity and drug discontinuation were higher in study 1 than in study 2. We consider that study 2 is more promising for the treatment of previously untreated advanced prostate cancer, although the rate of adverse side effects is still high as compared with other hormonal therapies. In order to overcome the high toxicity rate, especially the gastrointestinal toxicity, we recently elaborated a method employing tailor-made medicine using SNPs of 1A1 gene in cytochrome P-450 for decreasing the rate of gastrointestinal toxicity. Using this method of patient selection, study 3 has been successfully launched on September 2005 with high drug compliance. Better clinical results are being accumulated.

Active control of surge in centrifugal compressors using magnetic thrust bearing actuation

NASA Astrophysics Data System (ADS)

Sanadgol, Dorsa

This research presents a new method for active surge control in centrifugal compressors with unshrouded impellers using a magnetic thrust bearing to modulate the impeller tip clearance. Magnetic bearings offer the potential for active control of flow instabilities. This capability is highly dependent on the sensitivity of the compressor characteristics to blade tip clearance. If the position of the shaft can be actuated with sufficient authority and speed, the induced pressure modulation makes control of surge promising. The active nature of the magnetic bearing system makes the real-time static and dynamic positioning of the rotor and therefore modulation of the impeller tip clearance possible. A theoretical model is first established that describes the sensitivity of the centrifugal compressor characteristic curve to tip clearance variations induced by axial motion of the rotor. Results from simulation of the nonlinear model for a single stage high-speed centrifugal compressor show that using the proposed control method, mass flow and pressure oscillations associated with compressor surge are quickly suppressed with acceptable tip clearance excursions, typically less than 20% of the available clearance. It is shown that it is possible to produce adequate axial excursions in the clearance between the impeller blades and the adjacent stationary shroud using a magnetic thrust bearing with practical levels of drive voltage. This surge control method would allow centrifugal compressors to reliably and safely operate with a wider range than is currently done in the field. The principal advantage of the proposed approach over conventional surge control methods lies in that, in machines already equipped with magnetic bearing, the method can potentially be implemented by simply modifying controller software. This dispenses with the need to introduce additional hardware, permitting adaptation of existing machinery at virtually no cost. In addition, since the controller is designed with the objective of keeping the trajectories on the compressor characteristic curve, the compressor performance and efficiency are no longer sacrificed by excessive recycling to achieve stability. In order to explore these conjectures experimentally, a high speed centrifugal compressor test facility with active magnetic bearings is developed. The test facility can be used for implementing the proposed surge control method and also for assessing the impeller and bearing loads at off-design conditions. This data can then be used to verify and refine analytical models used in compressor design. (Abstract shortened by UMI.)

Ensemble projection of the sea level rise impact on storm surge and inundation at the coast of Bangladesh

NASA Astrophysics Data System (ADS)

Jisan, Mansur Ali; Bao, Shaowu; Pietrafesa, Leonard J.

2018-01-01

The hydrodynamic model Delft3D is used to study the impact of sea level rise (SLR) on storm surge and inundation in the coastal region of Bangladesh. To study the present-day inundation scenario, the tracks of two known tropical cyclones (TC) were used: Aila (Category 1; 2009) and Sidr (Category 5; 2007). Model results were validated with the available observations. Future inundation scenarios were generated by using the strength of TC Sidr, TC Aila and an ensemble of historical TC tracks but incorporating the effect of SLR. Since future change in storm surge inundation under SLR impact is a probabilistic incident, a probable range of future change in the inundated area was calculated by taking into consideration the uncertainties associated with TC tracks, intensities and landfall timing. The model outputs showed that the inundated area for TC Sidr, which was calculated as 1860 km2, would become 31 % larger than the present-day scenario if a SLR of 0.26 m occurred during the mid-21st-century climate scenario. Similarly to that, an increasing trend was found for the end-21st-century climate scenario. It was found that with a SLR of 0.54 m, the inundated area would become 53 % larger than the present-day case. Along with the inundation area, the impact of SLR was examined for changes in future storm surge level. A significant increase of 14 % was found in storm surge level for the case of TC Sidr at Barisal station if a SLR of 0.26 m occurred in the mid-21st century. Similarly to that, an increase of 29 % was found at storm surge level with a SLR of 0.54 m in this location for the end-21st-century climate scenario. Ensemble projections based on uncertainties of future TC events also showed that, for a change of 0.54 m in SLR, the inundated area would range between 3500 and 3750 km2, whereas for present-day SLR simulations it was found within the range of 1000-1250 km2. These results revealed that even if the future TCs remain at the same strength as at present, the projected changes in SLR will generate more severe threats in terms of surge height and the extent of the inundated area.

Future Projection of Storm Surge at Tokyo Bay under RCP 8.5 Scenario by Meteorological-Ocean-Tide Coupled Model

NASA Astrophysics Data System (ADS)

Iwamoto, T.; Nakamura, R.; Takagawa, T.; Shibayama, T.

2016-12-01

It is clearly valuable to accomplish well-reproduced storm surge model and conduct future projection for disaster prevention. In this study, the reproducibility of Meteorological-Ocean-Tide coupled model was validated by simulating typhoon Roke (2011) storm surge, which was recorded as the highest anomaly (119cm) at Tokyo tide station (JMA) in Tokyo Bay over the last 10 years. Furthermore, the future projection (2050) under global warming scenario (RCP8.5) was conducted. The coupled model was composed of 3 models; ARW-WRFV3 (Skamarock et al., 2008), FVCOM (Chen et al., 2011) and WXTide32. WRF firstly calculated downscaled meteorological field by using FiNal anaLysis (FNL) as initial/boundary (I/B) condition. In this calculation, single layer urban canopy model (Kusaka et al., 2001) and topography data from SRTM3 (90m mesh) and GSI (50m mesh) were applied. Then the output was used as I/B condition to FVCOM, which calculated storm surge. Finally tide level was calculated by adding storm surge to astronomical tide calculated by WXTide32. For 2050 case, sea surface temperature (SST) from 26 GCM under RCP8.5 was used for constructing pseudo global warming meteorological fields. In details, ensemble average of SST variation between 2006-2015 and 2041-2060 was added to FNL's SST by following Oya et al (2016). In this case, calculating astronomical tide is omitted due to the limitation of WXTide32. The reproduced result of typhoon Roke shows that the difference of maximum tide level (first peak) to the observation is less than 10cm, the difference of second peak is about 50cm. The future projection result shows that the increase of storm surge at Tokyo tide station is about 20cm and that at Funabashi is about 30cm. This intensification is mainly caused by wind speed increment, since the variation of low pressure due to higher SST is relatively small. Moreover, Funabashi is located in front of the open space at inner part of Tokyo Bay, Tokyo tide station is similar however is installed at Tokyo harbor which has intricate terrain. This implies that the geographical condition will affect storm surge significantly for typhoon Roke-like case.

Variation of Strom Surge Propagation in a Shallow Estuary with Sea Level Rise

NASA Astrophysics Data System (ADS)

Herrington, T. O., Jr.; Blumberg, A. F.

2014-12-01

Hurricane Sandy made landfall along the New Jersey coast at 8pm EDT on October 29th, 2012. At landfall wind gusts of between 129 and 145 km/hr were recorded in New York and New Jersey. The large wind field associated with the storm generated an extreme storm surge north of the eye at landfall resulting in high-velocity overland storm surge along the northern barrier Islands of the Barnegat Bay followed 7 hours later by a rapid rise in water level along the bayside of the barrier islands. A high-resolution, hydrodynamic model for the Barnegat Bay estuary; including its vast intertidal areas, has been developed and validated to simulate the observed Sandy storm surge. The Barnegat Bay Inundation Model (BBIMS) has a constant 100m resolution and is nested within the three dimensional Stevens NYHOPS ocean circulation model at its offshore open boundary. Wetting and drying of land features in the model's external time step is as low as 0.1 sec in its 2D barotropic mode. This mode provides for the dynamic prediction of depth integrated flood elevations and velocities across land features during inundation events. The BBIMS was calibrated using the NYHOPS hindcast of Hurricane Sandy. The hindcast utilized Sandy over ocean wind field and atmospheric pressure data, offshore wave and tidal boundary forcing, atmospheric heat fluxes, interior stream flow data and was validated against observed water levels and measured high water marks. A comparison against 6 water level time series measured by USGS tide gauges located in the Barnegat Bay verified that the model is able to capture the spatial and temporal variation of water levels in the Bay observed during Hurricane Sandy. A comparison against the verified high water marks found that the model is capable of hincasting overland water elevation to within 0.63ft (one standard deviation) at 71% of the total water marks measured. The modeling results show that strong northerly winds along the axis of the estuary prior to landfall suppressed the storm surge in the northern portion of the Bay. A rapid shift in wind direction to southerly winds after landfall allowed the surge to propagate north up the estuary as a shallow water wave (Figure 1). The effect of future sea levels on surge propagation in the estuary is investigated through increases in model mean sea level.

Hurricane Matthew (2016) and its Storm Surge Inundation under Global Warming Scenarios: Application of an Interactively Coupled Atmosphere-Ocean Model

NASA Astrophysics Data System (ADS)

Jisan, M. A.; Bao, S.; Pietrafesa, L.; Pullen, J.

2017-12-01

An interactively coupled atmosphere-ocean model was used to investigate the impacts of future ocean warming, both at the surface and the layers below, on the track and intensity of a hurricane and its associated storm surge and inundation. The category-5 hurricane Matthew (2016), which made landfall on the South Carolina coast of the United States, was used for the case study. Future ocean temperature changes and sea level rise (SLR) were estimated based on the projection of Inter-Governmental Panel on Climate Change (IPCC)'s Representative Concentration Pathway scenarios RCP 2.6 and RCP 8.5. After being validated with the present-day observational data, the model was applied to simulate the changes in track, intensity, storm surge and inundation that Hurricane Matthew would cause under future climate change scenarios. It was found that a significant increase in hurricane intensity, storm surge water level, and inundation area for Hurricane Matthew under future ocean warming and SLR scenarios. For example, under the RCP 8.5 scenario, the maximum wind speed would increase by 17 knots (14.2%), the minimum sea level pressure would decrease by 26 hPa (2.85%), and the inundated area would increase by 401 km2 (123%). By including the effect of SLR for the middle-21st-century scenario, the inundated area will further increase by up to 49.6%. The increase in the hurricane intensity and the inundated area was also found for the RCP 2.6 scenario. The response of sea surface temperature was analyzed to investigate the change in intensity. A comparison was made between the impacts when only the sea surface warming is considered versus when both the sea surface and the underneath layers are considered. These results showed that even without the effect of SLR, the storm surge level and the inundated area would be higher due to the increased hurricane intensity under the influence of the future warmer ocean temperature. The coupled effect of ocean warming and SLR would cause the hurricane-induced storm surge and inundation to be amplified. The relative importance of the ocean warming versus the SLR was evaluated. Keywords: Hurricane Matthew, Global Warming, Coupled Atmosphere-Ocean Model, Air-Sea interactions, Storm Surge, Inundation

Electro-optic analysis of the influence of target geometry on electromagnetic pulses generated by petawatt laser-matter interactions

NASA Astrophysics Data System (ADS)

Robinson, Timothy; Giltrap, Samuel; Eardley, Samuel; Consoli, Fabrizio; De Angelis, Riccardo; Ingenito, Francesco; Stuart, Nicholas; Verona, Claudio; Smith, Roland A.

2018-01-01

We present an analysis of strong laser-driven electromagnetic pulses using novel electro-optic diagnostic techniques. A range of targets were considered, including thin plastic foils (20-550 nm) and mass-limited, optically-levitated micro-targets. Results from foils indicate a dependence of EMP on target thickness, with larger peak electric fields observed with thinner targets. Spectral analysis suggests high repeatability between shots, with identified spectral features consistently detected with <1 MHz standard deviations of the peak position. This deviation is reduced for shots taken on the same day, suggesting that local conditions, such as movement of metal objects within the target chamber, are more likely to lead to minor spectral modifications, highlighting the role of the local environment in determining the details of EMP production. Levitated targets are electrically isolated from their environment, hence these targets should be unable to draw a neutralization current from the earth following ejection of hot electrons from the plasma, in contrast to predictions for pin-mounted foils in the Poyé EMP generation model. With levitated targets, no EMP was measurable above the noise threshold of any diagnostic, despite observation of protons accelerated to >30 MeV energies, suggesting the discharge current contribution to EMP is dominant.

Advanced imaging approaches for characterizing nanoparticle delivery and dispersion in skin (Conference Presentation)

NASA Astrophysics Data System (ADS)

Prow, Tarl W.; Yamada, Miko; Dang, Nhung; Evans, Conor L.

2017-02-01

The purpose of this research was to develop advanced imaging approaches to characterise the combination of elongated silica microparticles (EMP) and nanoparticles to control topical delivery of drugs and peptides. The microparticles penetrate through the epidermis and stop at the dermal-epidermal junction (DEJ). In this study we incorporated a fluorescent lipophilic dye, DiI, as a hydrophobic drug surrogate into the nanoparticle for visualization with microscopy. In another nanoparticle-based approach we utilized a chemically functionalized melanin nanoparticle for peptide delivery. These nanoparticles were imaged by coherent anti-Stoke Raman scattering (CARS) microscopy to characterize the delivery of these nanoparticles into freshly excised human skin. We compared four different coating approaches to combine EMP and nanoparticles. These data showed that a freeze-dried formulation with cross-linked alginate resulted in 100% of the detectable nanoparticle retained on the EMP. When this dry form of EMP-nanoparticle was applied to excised, living human abdominal skin, the EMP penetrated to the DEJ followed by controlled release of the nanoparticles. This formulation resulted in a sustained release profile, whereas a freeze-dried formulation without crosslinking showed an immediate burst-type release profile. These data show that advanced imaging techniques can give unique, label free data that shows promise for clinical investigations.

Increased nitric oxide synthase activity is essential for electromagnetic-pulse-induced blood-retinal barrier breakdown in vivo.

PubMed

Lu, Lianjun; Xu, Hui; Wang, Xiaowu; Guo, Guozhen

2009-04-06

To examine whether electromagnetic pulses (EMPs) affected the permeability of the blood-retinal barrier (BRB), gene expression of occludin and activity of nitric oxide synthase (NOS). Sprague-Dawley (SD) rats were used and randomized into EMP and control groups. Retinas were removed immediately, and 2 h or 24 h after EMP radiation. BRB permeability was analyzed by transmission electron microscopy and Evans Blue staining. Retinal NOS activity and concentrations of nitrite and nitrate were measured. Occludin mRNA and protein levels were detected by RT-PCR and Western blotting. Exposure of SD rats to EMP resulted in increased BRB permeability, with the greatest decrease in occludin at 24 h. Moreover, this permeability defect was also correlated with significant increases in the formation of NO and induction of NOS activity in SD rats. Furthermore, we found that treatment with NOS inhibitor N-nitro-L-arginine methyl ester (L-NAME) blocked BRB breakdown and prevented the increase in NO formation and induction of NOS activity, as well as the decrease in occluding expression. Taken together, these results support the view that NOS-dependent NO production is an important factor that contributes to EMP-induced BRB dysfunction, and suggests that NOS induction may play an important role in BRB breakdown.

The development of the time dependence of the nuclear EMP electric field

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eng, C

The nuclear electromagnetic pulse (EMP) electric field calculated with the legacy code CHAP is compared with the field given by an integral solution of Maxwell's equations, also known as the Jefimenko equation, to aid our current understanding on the factors that affect the time dependence of the EMP. For a fair comparison the CHAP current density is used as a source in the Jefimenko equation. At first, the comparison is simplified by neglecting the conduction current and replacing the standard atmosphere with a constant density air slab. The simplicity of the resultant current density aids in determining the factors thatmore » affect the rise, peak and tail of the EMP electric field versus time. The three dimensional nature of the radiating source, i.e. sources off the line-of-sight, and the time dependence of the derivative of the current density with respect to time are found to play significant roles in shaping the EMP electric field time dependence. These results are found to hold even when the conduction current and the standard atmosphere are properly accounted for. Comparison of the CHAP electric field with the Jefimenko electric field offers a direct validation of the high-frequency/outgoing wave approximation.« less

Something Old Something New

ERIC Educational Resources Information Center

Pierce, Dennis

2018-01-01

To help meet the surging demand for medical care as veterans returned home from World War II, Mississippi Gulf Coast Community College (MGCCC) created its first nursing program back in 1944. Since that time, the college has become a leader in local health care education--and a brand-new Nursing and Simulation Center will help further this legacy.…

Decision Manifold Approximation for Physics-Based Simulations

NASA Technical Reports Server (NTRS)

Wong, Jay Ming; Samareh, Jamshid A.

2016-01-01

With the recent surge of success in big-data driven deep learning problems, many of these frameworks focus on the notion of architecture design and utilizing massive databases. However, in some scenarios massive sets of data may be difficult, and in some cases infeasible, to acquire. In this paper we discuss a trajectory-based framework that quickly learns the underlying decision manifold of binary simulation classifications while judiciously selecting exploratory target states to minimize the number of required simulations. Furthermore, we draw particular attention to the simulation prediction application idealized to the case where failures in simulations can be predicted and avoided, providing machine intelligence to novice analysts. We demonstrate this framework in various forms of simulations and discuss its efficacy.

Application of Fuzzy-Logic Controller and Neural Networks Controller in Gas Turbine Speed Control and Overheating Control and Surge Control on Transient Performance

NASA Astrophysics Data System (ADS)

Torghabeh, A. A.; Tousi, A. M.

2007-08-01

This paper presents Fuzzy Logic and Neural Networks approach to Gas Turbine Fuel schedules. Modeling of non-linear system using feed forward artificial Neural Networks using data generated by a simulated gas turbine program is introduced. Two artificial Neural Networks are used , depicting the non-linear relationship between gas generator speed and fuel flow, and turbine inlet temperature and fuel flow respectively . Off-line fast simulations are used for engine controller design for turbojet engine based on repeated simulation. The Mamdani and Sugeno models are used to expression the Fuzzy system . The linguistic Fuzzy rules and membership functions are presents and a Fuzzy controller will be proposed to provide an Open-Loop control for the gas turbine engine during acceleration and deceleration . MATLAB Simulink was used to apply the Fuzzy Logic and Neural Networks analysis. Both systems were able to approximate functions characterizing the acceleration and deceleration schedules . Surge and Flame-out avoidance during acceleration and deceleration phases are then checked . Turbine Inlet Temperature also checked and controls by Neural Networks controller. This Fuzzy Logic and Neural Network Controllers output results are validated and evaluated by GSP software . The validation results are used to evaluate the generalization ability of these artificial Neural Networks and Fuzzy Logic controllers.

Assessing the Impact of Topography on Groundwater Salinization Due to Storm Surge Inundation

NASA Astrophysics Data System (ADS)

Yu, X.; Yang, J.; Graf, T.; Koneshloo, M.; O'Neal, M. A.; Michael, H. A.

2015-12-01

The sea-level rise and increase in the frequency and intensity of coastal storms due to climate change are likely to exacerbate adverse effects of storm surges on low-lying coastal areas. The landward flow of water during storm surges introduces salt to surficial coastal aquifers and threatens groundwater resources. Coastal topography (e.g. ponds, dunes, canals) likely has a strong impact on overwash and salinization processes, but is generally highly simplified in modeling studies. To understand the topographic impacts on groundwater salinization, we modeled overwash and variable-density groundwater flow and salt transport in 3D using the fully coupled surface and subsurface numerical simulator, HydroGeoSphere. The model simulates the coastal aquifer as an integrated system considering processes such as overland flow, coupled surface and subsurface exchange, variably saturated flow, and variable-density flow. To represent various coastal landscape types, we started with realistic coastal topography from Delaware, USA, and then generated synthetic fields with differing shore-perpendicular connectivity and surface depressions. The groundwater salinization analysis suggested that the topographic connectivity promoting overland flow controls the volume of aquifer that is salinized. In contrast, depression storage of surface water mainly controls the time for infiltrated salt to flush from the aquifer. The results indicate that for a range of synthetic conditions, topography increases the flushing time of salt by 20-300% relative to an equivalent "simple slope" in which topographic variation is absent. Our study suggests that topography have a significant impact on overwash salinization, with important implications for land management at local scales and groundwater vulnerability assessment at regional to global scales.

KSC-2009-2308

NASA Image and Video Library

2009-03-26

CAPE CANAVERAL, Fla. – The NASA Railroad hauls one of the cars with the first Ares I-X segment to the Rotation, Processing and Surge Facility at NASA's Kennedy Space Center in Florida. Four reusable motor segments and the nozzle exit cone, manufactured by the Ares I first-stage prime contractor Alliant Techsystems Inc., departed Utah March 12 on the seven-day, cross-country trip to Florida. The segments are being delivered to Kennedy's Rotation, Processing and Surge Facility for final processing and integration. The booster used for the Ares I-X launch is being modified by adding new forward structures and a fifth segment simulator. The motor is the final hardware needed for the rocket's upcoming test flight this summer. The stacking operations are scheduled to begin in the Vehicle Assembly Building in April. Photo credit: NASA/Jack Pfaller

KSC-2009-2307

NASA Image and Video Library

2009-03-26

CAPE CANAVERAL, Fla. – The NASA Railroad hauls one of the cars with the first Ares I-X segment to the Rotation, Processing and Surge Facility at NASA's Kennedy Space Center in Florida. Four reusable motor segments and the nozzle exit cone, manufactured by the Ares I first-stage prime contractor Alliant Techsystems Inc., departed Utah March 12 on the seven-day, cross-country trip to Florida. The segments are being delivered to Kennedy's Rotation, Processing and Surge Facility for final processing and integration. The booster used for the Ares I-X launch is being modified by adding new forward structures and a fifth segment simulator. The motor is the final hardware needed for the rocket's upcoming test flight this summer. The stacking operations are scheduled to begin in the Vehicle Assembly Building in April. Photo credit: NASA/Jack Pfaller

KSC-2009-2310

NASA Image and Video Library

2009-03-26

CAPE CANAVERAL, Fla. – The NASA Railroad delivers the first Ares I-X segment to the Rotation, Processing and Surge Facility at NASA's Kennedy Space Center in Florida. Four reusable motor segments and the nozzle exit cone, manufactured by the Ares I first-stage prime contractor Alliant Techsystems Inc., departed Utah March 12 on the seven-day, cross-country trip to Florida. The segments are being delivered to Kennedy's Rotation, Processing and Surge Facility for final processing and integration. The booster used for the Ares I-X launch is being modified by adding new forward structures and a fifth segment simulator. The motor is the final hardware needed for the rocket's upcoming test flight this summer. The stacking operations are scheduled to begin in the Vehicle Assembly Building in April. Photo credit: NASA/Jack Pfaller

KSC-2009-2309

NASA Image and Video Library

2009-03-26

CAPE CANAVERAL, Fla. – The NASA Railroad delivers the first Ares I-X segment to the Rotation, Processing and Surge Facility at NASA's Kennedy Space Center in Florida. Four reusable motor segments and the nozzle exit cone, manufactured by the Ares I first-stage prime contractor Alliant Techsystems Inc., departed Utah March 12 on the seven-day, cross-country trip to Florida. The segments are being delivered to Kennedy's Rotation, Processing and Surge Facility for final processing and integration. The booster used for the Ares I-X launch is being modified by adding new forward structures and a fifth segment simulator. The motor is the final hardware needed for the rocket's upcoming test flight this summer. The stacking operations are scheduled to begin in the Vehicle Assembly Building in April. Photo credit: NASA/Jack Pfaller

Radiotherapy in the treatment of solitary plasmacytoma.

PubMed

Jyothirmayi, R; Gangadharan, V P; Nair, M K; Rajan, B

1997-05-01

Solitary plasmacytoma of bone (SPB) and extramedullary plasmacytoma (EMP) are rare. High local control rates are reported with radiotherapy, although the optimal dose and extent of radiotherapy portals remains controversial. Between 1983 and 1993, 30 patients with solitary plasmacytoma were seen at the Regional Cancer Centre, Trivandrum, India. 23 patients had SPB and seven EMP. The mean age was 52 years and the male to female ratio 3.2:1. Diagnosis of SPB was confirmed by biopsy in 16 patients and tumour excision in seven. 20 patients received megavoltage radiotherapy to the bone lesion with limited margins, and one received chemotherapy. Two patients who underwent complete tumour excision received no further treatment. All seven patients with EMP received megavoltage radiotherapy, four following biopsy and three after tumour excision. Local control was achieved in all patients with SPB. Nine progressed to multiple myeloma and one developed a solitary plasmacytoma in another bone. Six patients with EMP achieved local control. Three later progressed to multiple myeloma and one had local relapse. Median time to relapse was 28 months in SPB and 30 months in EMP. 5-year overall survival rates were 82% and 57% for patients with SPB and EMP, respectively. The corresponding progression free survival rates were 55% and 50%, respectively. Age, sex, site of tumour, serum M protein and haemoglobin levels did not significantly influence progression free survival. The extent of surgery, radiotherapy dose or time to relapse were not significant prognostic factors. Radiotherapy appears to be an effective modality of treatment of solitary plasmacytoma. No dose-response relationship is observed, and high local control rates are achieved with limited portals. Progression to multiple myeloma is the commonest pattern of failure, although no prognostic factors for progression are identified. The role of chemotherapy in preventing disease progression needs further evaluation.

Lung-Derived Microscaffolds Facilitate Diabetes Reversal after Mouse and Human Intraperitoneal Islet Transplantation.

PubMed

Abualhassan, Nasser; Sapozhnikov, Lena; Pawlick, Rena L; Kahana, Meygal; Pepper, Andrew R; Bruni, Antonio; Gala-Lopez, Boris; Kin, Tatsuya; Mitrani, Eduardo; Shapiro, A M James

2016-01-01

There is a need to develop three-dimensional structures that mimic the natural islet tissue microenvironment. Endocrine micro-pancreata (EMPs) made up of acellular organ-derived micro-scaffolds seeded with human islets have been shown to express high levels of key beta-cell specific genes and secrete quantities of insulin per cell similar to freshly isolated human islets in a glucose-regulated manner for more than three months in vitro. The aim of this study was to investigate the capacity of EMPs to restore euglycemia in vivo after transplantation of mouse or human islets in chemically diabetic mice. We proposed that the organ-derived EMPs would restore the extracellular components of the islet microenvironment, generating favorable conditions for islet function and survival. EMPs seeded with 500 mouse islets were implanted intraperitoneally into streptozotocin-induced diabetic mice and reverted diabetes in 67% of mice compared to 13% of controls (p = 0.018, n = 9 per group). Histological analysis of the explanted grafts 60 days post-transplantation stained positive for insulin and exhibited increased vascular density in a collagen-rich background. EMPs were also seeded with human islets and transplanted into the peritoneal cavity of immune-deficient diabetic mice at 250 islet equivalents (IEQ), 500 IEQ and 1000 IEQ. Escalating islet dose increased rates of normoglycemia (50% of the 500 IEQ group and 75% of the 1000 IEQ group, n = 3 per group). Human c-peptide levels were detected 90 days post-transplantation in a dose-response relationship. Herein, we report reversal of diabetes in mice by intraperitoneal transplantation of human islet seeded on EMPs with a human islet dose as low as 500 IEQ.

Lung-Derived Microscaffolds Facilitate Diabetes Reversal after Mouse and Human Intraperitoneal Islet Transplantation

PubMed Central

Pawlick, Rena L.; Kahana, Meygal; Pepper, Andrew R.; Bruni, Antonio; Gala-Lopez, Boris; Kin, Tatsuya; Mitrani, Eduardo; Shapiro, A. M. James

2016-01-01

There is a need to develop three-dimensional structures that mimic the natural islet tissue microenvironment. Endocrine micro-pancreata (EMPs) made up of acellular organ-derived micro-scaffolds seeded with human islets have been shown to express high levels of key beta-cell specific genes and secrete quantities of insulin per cell similar to freshly isolated human islets in a glucose-regulated manner for more than three months in vitro. The aim of this study was to investigate the capacity of EMPs to restore euglycemia in vivo after transplantation of mouse or human islets in chemically diabetic mice. We proposed that the organ-derived EMPs would restore the extracellular components of the islet microenvironment, generating favorable conditions for islet function and survival. EMPs seeded with 500 mouse islets were implanted intraperitoneally into streptozotocin-induced diabetic mice and reverted diabetes in 67% of mice compared to 13% of controls (p = 0.018, n = 9 per group). Histological analysis of the explanted grafts 60 days post-transplantation stained positive for insulin and exhibited increased vascular density in a collagen-rich background. EMPs were also seeded with human islets and transplanted into the peritoneal cavity of immune-deficient diabetic mice at 250 islet equivalents (IEQ), 500 IEQ and 1000 IEQ. Escalating islet dose increased rates of normoglycemia (50% of the 500 IEQ group and 75% of the 1000 IEQ group, n = 3 per group). Human c-peptide levels were detected 90 days post-transplantation in a dose-response relationship. Herein, we report reversal of diabetes in mice by intraperitoneal transplantation of human islet seeded on EMPs with a human islet dose as low as 500 IEQ. PMID:27227978

[Effects of electromagnetic pulse on blood-brain barrier permeability and tight junction proteins in rats].

PubMed

Qiu, Lian-bo; Ding, Gui-rong; Zhang, Ya-mei; Zhou, Yan; Wang, Xiao-wu; Li, Kang-chu; Xu, Sheng-long; Tan, Juan; Zhou, Jia-xing; Guo, Guo-zhen

2009-09-01

To study the effect of electromagnetic pulse (EMP) on the permeability of blood-brain barrier, tight junction (TJ)-associated protein expression and localization in rats. 66 male SD rats, weighing (200 approximately 250) g, were sham or whole-body exposed to EMP at 200 kV/m for 200 pulses. The repetition rate was 1 Hz. The permeability of the blood-brain barrier in rats was assessed by albumin immunohistochemistry. The expression of typical tight junction protein ZO-1 and occludin in both cerebral cortex homogenate and cerebral cortex microvessel homogenate was analyzed by the Western blotting and the distribution of ZO-1 and occludin was examined by immunofluorescence microscopy. In the sham exposure rats, no brain capillaries showed albumin leakage, at 0.5 h after 200 kV/m EMP exposure for 200 pulses; a few brain capillaries with extravasated serum albumin was found, with the time extended, the number of brain capillaries with extravasated serum albumin increased, and reached the peak at 3 h, then began to recover at 6 h. In addition, no change in the distribution of the occludin was found after EMP exposure. Total occludin expression had no significant change compared with the control. However, the expression level of ZO-1 significantly decreased at 1 h and 3 h after EMP exposure in both cerebral cortex homogenate and cerebral cortex microvessel homogenate. Furthermore, immunofluorescence studies also showed alterations in ZO-1 protein localization in cerebral cortex microvessel. The EMP exposure (200 kV/m, 200 pulses) could increase blood-brain barrier permeability in rat, and this change is associated with specific alterations in tight junction protein ZO-1.

Recombinant tissue plasminogen activator enhances microparticle release from mouse brain-derived endothelial cells through plasmin.

PubMed

Garraud, Marie; Khacef, Kahina; Vion, Anne-Clémence; Leconte, Claire; Yin, Min; Renard, Jean-Marie; Marchand-Leroux, Catherine; Boulanger, Chantal M; Margaill, Isabelle; Beray-Berthat, Virginie

2016-11-15

Thrombolysis with recombinant tissue plasminogen activator (rt-PA) is currently the only approved pharmacological strategy for acute ischemic stroke. However, rt-PA exhibits vascular toxicity mainly due to endothelial damage. To investigate the mechanisms underlying rt-PA-induced endothelial alterations, we assessed the role of rt-PA in the generation of endothelial microparticles (EMPs), emerging biological markers and effectors of endothelial dysfunction. The mouse brain-derived endothelial cell line bEnd.3 was used. Cells were treated with rt-PA at 20, 40 or 80μg/ml for 15 or 24h, and EMPs were quantified in the culture media using Annexin-V staining coupled with flow cytometry. Rt-PA enhanced EMP release from bEnd.3 cells with a maximal increase at the 40μg/ml dose for 24h (+78% compared to controls). Using tranexamic acid and aprotinin we demonstrated that plasmin is responsible for rt-PA-induced EMP release. The p38 MAPK inhibitor SB203580 and the poly(ADP-ribose)polymerase (PARP) inhibitor PJ34 also reduced rt-PA-induced EMP production, suggesting that p38 MAPK and PARP are downstream intracellular effectors of rt-PA/plasmin. Rt-PA also altered through plasmin the morphology and the confluence of bEnd.3 cells. By contrast, these changes did not implicate p38 MAPK and PARP. This study demonstrates that rt-PA induces the production of microparticles by cerebral endothelial cells, through plasmin, p38 MAPK and PARP pathways. Determining the phenotype of these EMPs to clarify their role on the endothelium in ischemic conditions could thus be of particular interest. Copyright © 2016 Elsevier B.V. All rights reserved.

Abundance profiling of extremely metal-poor stars and supernova properties in the early universe

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tominaga, Nozomu; Iwamoto, Nobuyuki; Nomoto, Ken'ichi, E-mail: tominaga@konan-u.ac.jp, E-mail: iwamoto.nobuyuki@jaea.go.jp, E-mail: nomoto@astron.s.u-tokyo.ac.jp

2014-04-20

After the big bang nucleosynthesis, the first heavy element enrichment in the universe was made by a supernova (SN) explosion of a population (Pop) III star (Pop III SN). The abundance ratios of elements produced from Pop III SNe are recorded in abundance patterns of extremely metal-poor (EMP) stars. The observations of the increasing number of EMP stars have made it possible to statistically constrain the explosion properties of Pop III SNe. We present Pop III SN models whose nucleosynthesis yields well reproduce, individually, the abundance patterns of 48 such metal-poor stars as [Fe/H] ≲ – 3.5. We then derivemore » relations between the abundance ratios of EMP stars and certain explosion properties of Pop III SNe: the higher [(C + N)/Fe] and [(C + N)/Mg] ratios correspond to the smaller ejected Fe mass and the larger compact remnant mass, respectively. Using these relations, the distributions of the abundance ratios of EMP stars are converted to those of the explosion properties of Pop III SNe. Such distributions are compared with those of the explosion properties of present day SNe: the distribution of the ejected Fe mass of Pop III SNe has the same peak as that of the present day SNe but shows an extended tail down to ∼10{sup –2}-10{sup –5} M {sub ☉}, and the distribution of the mass of the compact remnant of Pop III SNe is as wide as that of the present-day, stellar-mass black holes. Our results demonstrate the importance of large samples of EMP stars obtained by ongoing and future EMP star surveys and subsequent high-dispersion spectroscopic observations in clarifying the nature of Pop III SNe in the early universe.« less