Impact test of a crash-energy management passenger rail car

DOT National Transportation Integrated Search

2004-04-06

On December 3, 2003, a single-car impact test was : conducted to assess the crashworthiness performance of a : modified passenger rail car. A coach car retrofitted with a : Crash Energy Management (CEM) end structure impacted a : fixed barrier at app...

Crash energy management : one- and two-car passenger rail impact tests - summary of structural and occupant test results

DOT National Transportation Integrated Search

2016-12-01

Two full-scale impact tests were conducted to measure the crashworthiness performance of Crash Energy Management (CEM) equipped passenger rail cars. On December 3, 2003, a single car impacted a fixed barrier at approximately 35 mph and on February 26...

Impact tests of crash energy management passenger rail cars: analysis and structural measurements

DOT National Transportation Integrated Search

2004-11-13

Two full-scale impact tests were conducted to measure the : crashworthiness performance of Crash Energy Management : (CEM) passenger rail cars. On December 3, 2003 a single car : impacted a fixed barrier at approximately 35 mph and on : February 26, ...

49 CFR 232.611 - Periodic maintenance.

Code of Federal Regulations, 2012 CFR

2012-10-01

... wiring and brackets; (2) ECP brake system electrical connections; and (3) Car mounted ECP brake system components. (b) Single car air brake test procedures. Prior to placing a freight car equipped with an ECP... car equipped with an ECP brake system shall be inspected and repaired before being released from a...

49 CFR 232.611 - Periodic maintenance.

Code of Federal Regulations, 2014 CFR

2014-10-01

... wiring and brackets; (2) ECP brake system electrical connections; and (3) Car mounted ECP brake system components. (b) Single car air brake test procedures. Prior to placing a freight car equipped with an ECP... car equipped with an ECP brake system shall be inspected and repaired before being released from a...

49 CFR 232.611 - Periodic maintenance.

Code of Federal Regulations, 2013 CFR

2013-10-01

... wiring and brackets; (2) ECP brake system electrical connections; and (3) Car mounted ECP brake system components. (b) Single car air brake test procedures. Prior to placing a freight car equipped with an ECP... car equipped with an ECP brake system shall be inspected and repaired before being released from a...

49 CFR 232.611 - Periodic maintenance.

Code of Federal Regulations, 2011 CFR

2011-10-01

... wiring and brackets; (2) ECP brake system electrical connections; and (3) Car mounted ECP brake system components. (b) Single car air brake test procedures. Prior to placing a freight car equipped with an ECP... car equipped with an ECP brake system shall be inspected and repaired before being released from a...

49 CFR 232.611 - Periodic maintenance.

Code of Federal Regulations, 2010 CFR

2010-10-01

... wiring and brackets; (2) ECP brake system electrical connections; and (3) Car mounted ECP brake system components. (b) Single car air brake test procedures. Prior to placing a freight car equipped with an ECP... car equipped with an ECP brake system shall be inspected and repaired before being released from a...

Dual-Pump Coherent Anti-Stokes Raman Scattering Temperature and CO2 Concentration Measurements

NASA Technical Reports Server (NTRS)

Lucht, Robert P.; Velur-Natarajan, Viswanathan; Carter, Campbell D.; Grinstead, Keith D., Jr.; Gord, James R.; Danehy, Paul M.; Fiechtner, G. J.; Farrow, Roger L.

2003-01-01

Measurements of temperature and CO2 concentration using dual-pump coherent anti-Stokes Raman scattering, (CARS) are described. The measurements were performed in laboratory flames,in a room-temperature gas cell, and on an engine test stand at the U.S. Air Force Research Laboratory, Wright-Patterson Air Force Base. A modeless dye laser, a single-mode Nd:YAG laser, and an unintensified back-illuminated charge-coupled device digital camera were used for these measurements. The CARS measurements were performed on a single-laser-shot basis. The standard deviations of the temperatures and CO2 mole fractions determined from single-shot dual-pump CARS spectra in steady laminar propane/air flames were approximately 2 and 10% of the mean values of approximately 2000 K and 0.10, respectively. The precision and accuracy of single-shot temperature measurements obtained from the nitrogen part of the dual-pump CARS system were investigated in detail in near-adiabatic hydrogen/air/CO2 flames. The precision of the CARS temperature measurements was found to be comparable to the best results reported in the literature for conventional two-laser, single-pump CARS. The application of dual-pump CARS for single-shot measurements in a swirl-stabilized combustor fueled with JP-8 was also demonstrated.

49 CFR 232.305 - Single car air brake tests.

Code of Federal Regulations, 2010 CFR

2010-10-01

... document at the Federal Railroad Administration, Docket Clerk, 1200 New Jersey Avenue, SE., Washington, DC... brake left applied; (ii) Slid flat wheel, unless known to be caused by hand brake left applied; or (iii... shall be performed on each new or rebuilt car prior to placing or using the car in revenue service. [66...

49 CFR 232.305 - Single car air brake tests.

Code of Federal Regulations, 2011 CFR

2011-10-01

... document at the Federal Railroad Administration, Docket Clerk, 1200 New Jersey Avenue, SE., Washington, DC... brake left applied; (ii) Slid flat wheel, unless known to be caused by hand brake left applied; or (iii... shall be performed on each new or rebuilt car prior to placing or using the car in revenue service. [66...

49 CFR 220.5 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... equipment, or track motor car, singly or in combination with other equipment, on the track of a railroad... systems, electric traction systems, roadway facilities or roadway maintenance machinery on or near track... one or more locomotives coupled with or without cars, requiring an air brake test in accordance with...

Predicting injury risk with "New Car Assessment Program" crashworthiness ratings.

PubMed

Jones, I S; Whitfield, R A

1988-12-01

The relationship between crashworthiness ratings produced by the National Highway Traffic Safety Administration's (NHTSA's) New Car Assessment Program (NCAP) and the risk of incapacitating injury or death for drivers who are involved in single-car, fixed-object, frontal collisions was examined. The results are based on 6,405 such crashes from the Motor Vehicle Traffic Accident file of the Texas Department of Highways and Public Transportation. The risk of injury was modeled using logistic regression taking into account the NCAP test results for each individual model of car and the intervening effects of car mass, age of the driver, restraint use, and crash severity. Three measures of anthropometric dummy response, Head Injury Criterion (HIC), Chest Deceleration (CD), and femur load were used to indicate vehicle crash test performance. The results show that there is a significant relationship between the results of the NCAP tests and the risk of serious injury or death in actual single-car frontal accidents. In terms of overall injury, chest deceleration was a better predictor than the Head Injury Criterion. For restrained drivers, crash severity, driver age, and chest deceleration were significant parameters for predicting risk of serious injury or death; the risk of injury decreased as chest deceleration decreased. The results were similar for unrestrained drivers although vehicle mass and femur load were also significant factors in the model. The risk of overall injury decreased as chest deceleration decreased but appeared to decrease as femur load increased.

49 CFR 180.519 - Periodic retest and inspection of tank cars other than single-unit tank car tanks.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Periodic retest and inspection of tank cars other than single-unit tank car tanks. 180.519 Section 180.519 Transportation Other Regulations Relating to... of Tank Cars § 180.519 Periodic retest and inspection of tank cars other than single-unit tank car...

Car suspension system monitoring under road conditions

NASA Astrophysics Data System (ADS)

Fedotov, A. I.; Kuznetsov, N. Y.; Lysenko, A. V.; Vlasov, V. G.

2017-12-01

The paper describes an advanced gyro-based measuring system comprising a CGV-4K central vertical gyro and a G-3M gyrocompass. The advanced system provides additional functions that help measure unsprung mass rotation angles about a vertical axis, rolling angles, trim angles and movements of the unsprung masses of the front (ap and al) and rear b axes when a car wheel hits a single obstruction. The paper also describes the operation of the system, which measures movements of unsprung masses about the body of a car when it hits a single obstruction. The paper presents the dependency diagrams ap = f(t) and al = f(t) for front and rear wheels respectively, as well as b = f(t) for a rear left wheel, which were determined experimentally. Test results for a car equipped with an advanced gyro-based measuring system moving around a circle can form a basis for developing a mathematical model of the process.

CARS Temperature Measurements in a Hypersonic Propulsion Test Facility

NASA Technical Reports Server (NTRS)

Jarrett, Olin, Jr.; Smith, M. W.; Antcliff, R. R.; Northam, G. Burt; Cutler, A. D.; Capriotti, D. P.; Taylor, D. J.

1990-01-01

Nonintrusive diagnostic measurements were performed in the supersonic reacting flow of the Hypersonic Propulsion Test Cell 2 at NASA-Langley. A Coherent Anti-stokes Raman Spectroscopy (CARS) system was assembled specifically for the test cell environment. System design considerations were: (1) test cell noise and vibration; (2) contamination from flow field or atmospheric borne dust; (3) unwanted laser or electrically induced combustion (inside or outside the duct); (4) efficient signal collection; (5) signal splitting to span the wide dynamic range present throughout the flow field; (6) movement of the sampling volume in the flow; and (7) modification of the scramjet model duct to permit optical access to the reacting flow with the CARS system. The flow in the duct was a nominal Mach 2 flow with static pressure near one atmosphere. A single perpendicular injector introduced hydrogen into the flow behind a rearward facing step. CARS data was obtained in three planes downstream of the injection region. At least 20 CARS data points were collected at each of the regularly spaced sampling locations in each data plane. Contour plots of scramjet combustor static temperature in a reacting flow region are presented.

Single passenger rail car impact test. Volume III, Test procedures, instrumentation and data.

DOT National Transportation Integrated Search

2000-01-12

A full-scale impact test was performed November 16, 1999, at the Federal Railroad Administrations Transportation : Technology Center, Pueblo, Colorado, by Transportation Technology Center, Inc., a subsidiary of the Association of : American Railro...

Mobile CARS - IRS Instrument for Simultaneous Spectroscopic Measurement of Multiple Properties in Gaseous Flows

NASA Technical Reports Server (NTRS)

Bivolaru, Daniel; Lee, Joseph W.; Jones, Stephen B.; Tedder, Sarah A.; Danehy, Paul M.; Weikl, M. C.; Magnotti, G.; Cutler, Andrew D.

2007-01-01

This paper describes a measurement system based on the dual-pump coherent anti-Stokes Raman spectroscopy (CARS) and interferometric Rayleigh scattering (IRS) methods. The IRS measurement is performed simultaneously with the CARS measurement using a common green laser beam as a narrow-band light source. The mobile CARS-IRS instrument is designed for the use both in laboratories as well as in ground-based combustion test facilities. Furthermore, it is designed to be easily transported between laboratory and test facility. It performs single-point spatially and temporally resolved simultaneous measurements of temperature, species mole fraction of N2, O2, and H2, and two-components of velocity. A mobile laser system can be placed inside or outside the test facility, while a beam receiving and monitoring system is placed near the measurement location. Measurements in a laboratory small-scale Mach 1.6 H2-air combustion-heated supersonic jet were performed to test the capability of the system. Final setup and pretests of a larger scale reacting jet are ongoing at NASA Langley Research Center s Direct Connect Supersonic Combustor Test Facility (DCSCTF).

Compatibility problems in frontal, side, single car collisions and car-to-pedestrian accidents in Japan.

PubMed

Mizuno, K; Kajzer, J

1999-07-01

Compatibility problems in car-to-car frontal, side, single car and car-to-pedestrian collisions in Japan are discussed using traffic accident data. The number of serious and fatal injuries is investigated for the subject car and other cars, which are categorized by their class and mass. The aggressivity of the cars is calculated by the number of fatalities, fatality rates and by the number of car registrations. The results show that in car-to-car frontal collisions, cars with a mass of 1150 kg are the most compatible among the current car population. In both car-to-car frontal and side collisions, the sports utility vehicle and mini car are found to be the most incompatible car types with high and low aggressivity, respectively. On the other hand, the accident data show that the wagon and midsize sedan are the most compatible car types. The compatibility of fixed objects in the road environment with cars and cars with pedestrians is also discussed. In a single car collision with a fixed object, the guardrail is the most compatible object and can reduce the fatality rate on prefecture roads by about 60%. The front geometry of the car has large effect on compatibility with a pedestrian.

Single passenger rail car impact test. Volume 3 : test procedures, instrumentation and data : rail passenger equipment collision tests

DOT National Transportation Integrated Search

2000-05-24

A full-scale impact test was performed November 16, 1999, at the Federal Railroad Administration's Transportation Technology Center, Pueblo, Colorado, by Transportation Technology Center, Inc., a subsidiary of the Association of American Railroads. T...

Lateral Stability Simulation of a Rail Truck on Roller Rig

NASA Astrophysics Data System (ADS)

Dukkipati, Rao V.

The development of experimental facilities for rail vehicle testing is being complemented by analytic studies. The purpose of this effort has been to gain insight into the dynamics of rail vehicles in order to guide development of the Roller Rigs and to establish an analytic framework for the design and interpretation of tests to be conducted on Roller Rigs. The work described here represents initial efforts towards meeting these objectives. Generic linear models were developed of a freight car (with a characteristic North American three-piece truck) on tangent track. The models were developed using the generalized multi body dynamics software MEDYNA. Predictions were made of the theoretical linear model hunting (lateral stability) characteristics of the freight car, i. e., the critical speeds and frequencies, for five different configurations: (a) freight car on track, (b) the freight car's front truck on the roller stand and its rear truck on track, (c) freight car on the roller rig, (d) a single truck on track, and (e) single truck on the roller stand. These were compared with the Association of American Railroads' field test data for an 80-ton hopper car equipped with A-3 ride control trucks. Agreement was reached among all the analytical models, with all models indicating a range of hunting speeds of 2% from the highest to lowest. The largest discrepancy, approximately 6%, was indicated between the models and the field test data. Parametric study results using linear model of freight truck on the roller rig show that (a) increasing roller radius increases critical speed (b) increasing the wheel initial cone angle will decrease the hunting speed (c) increasing the roller cant increases hunting speed (d) decrowning of the wheelset on the rollers will not effect the hunting speed but induces longitudinal destabilizing horizontal forces at the contact and (e) lozenging of wheelset on the rollers induces a yaw moment and the hunting speed decreases with increasing wheelset yaw angle.

In vitro anti-MRSA activity of carvone with gentamicin.

PubMed

Mun, Su-Hyun; Kang, Ok-Hwa; Joung, Dae-Ki; Kim, Sung-Bae; Choi, Jang-Gi; Shin, Dong-Won; Kwon, Dong-Yeul

2014-04-01

Carvone is one of the naturally occurring monoterpenes, the largest class of secondary metabolites in plants, and exists in two enantiomers, R-carvone (R-car) and S-car. The objective of this study was to investigate the antimicrobial activity of R-car and S-car with gentamicin (GET) against methicillin-resistant Staphylococcus aureus (MRSA). MRSA is a major human pathogen that causes serious problems, including hospital-acquired pneumonia, abscesses and surgical wound infections. Nosocomial MRSA infections often exhibit multidrug resistance. In the present study, antimicrobial susceptibility testing was performed with R-car, S-car and GET using the broth microdilution method. Minimal inhibitory concentration values for R- and S-car against six different strains of S. aureus ranged between 500 and 1,000 μg/ml. Anti-MRSA activity was evaluated using the checkerboard and time-kill assays to investigate the potential synergistic effects of different combinations of the carvone enantiomers and GET. R-car plus S-car, R-car plus GET and S-car plus GET exhibited significant synergistic activity against MRSA. These findings suggest that the single-agent anti-MRSA activities of R-car, S-car and GET are effectively increased through combination therapy. This study showed that carvone may be a potential adjuvant antimicrobial agent.

In vitro anti-MRSA activity of carvone with gentamicin

PubMed Central

MUN, SU-HYUN; KANG, OK-HWA; JOUNG, DAE-KI; KIM, SUNG-BAE; CHOI, JANG-GI; SHIN, DONG-WON; KWON, DONG-YEUL

2014-01-01

Carvone is one of the naturally occurring monoterpenes, the largest class of secondary metabolites in plants, and exists in two enantiomers, R-carvone (R-car) and S-car. The objective of this study was to investigate the antimicrobial activity of R-car and S-car with gentamicin (GET) against methicillin-resistant Staphylococcus aureus (MRSA). MRSA is a major human pathogen that causes serious problems, including hospital-acquired pneumonia, abscesses and surgical wound infections. Nosocomial MRSA infections often exhibit multidrug resistance. In the present study, antimicrobial susceptibility testing was performed with R-car, S-car and GET using the broth microdilution method. Minimal inhibitory concentration values for R- and S-car against six different strains of S. aureus ranged between 500 and 1,000 μg/ml. Anti-MRSA activity was evaluated using the checkerboard and time-kill assays to investigate the potential synergistic effects of different combinations of the carvone enantiomers and GET. R-car plus S-car, R-car plus GET and S-car plus GET exhibited significant synergistic activity against MRSA. These findings suggest that the single-agent anti-MRSA activities of R-car, S-car and GET are effectively increased through combination therapy. This study showed that carvone may be a potential adjuvant antimicrobial agent. PMID:24669246

49 CFR 180.519 - Periodic retest and inspection of tank cars other than single-unit tank car tanks.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 3 2011-10-01 2011-10-01 false Periodic retest and inspection of tank cars other than single-unit tank car tanks. 180.519 Section 180.519 Transportation Other Regulations Relating to... (CONTINUED) CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars...

49 CFR 180.519 - Periodic retest and inspection of tank cars other than single-unit tank car tanks.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Periodic retest and inspection of tank cars other than single-unit tank car tanks. 180.519 Section 180.519 Transportation Other Regulations Relating to... (CONTINUED) CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars...

49 CFR 180.519 - Periodic retest and inspection of tank cars other than single-unit tank car tanks.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 3 2012-10-01 2012-10-01 false Periodic retest and inspection of tank cars other than single-unit tank car tanks. 180.519 Section 180.519 Transportation Other Regulations Relating to... (CONTINUED) CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars...

49 CFR 180.519 - Periodic retest and inspection of tank cars other than single-unit tank car tanks.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 3 2014-10-01 2014-10-01 false Periodic retest and inspection of tank cars other than single-unit tank car tanks. 180.519 Section 180.519 Transportation Other Regulations Relating to... (CONTINUED) CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars...

Crash protection of stock car racing drivers--application of biomechanical analysis of Indy car crash research.

PubMed

Melvin, John W; Begeman, Paul C; Faller, Ronald K; Sicking, Dean L; McClellan, Scott B; Maynard, Edwin; Donegan, Michael W; Mallott, Annette M; Gideon, Thomas W

2006-11-01

Biomechanical analysis of Indy car crashes using on-board impact recorders (Melvin et al. 1998, Melvin et al. 2001) indicates that Indy car driver protection in high-energy crashes can be achieved in frontal, side, and rear crashes with severities in the range of 100 to 135 G peak deceleration and velocity changes in the range of 50 to 70 mph. These crashes were predominantly single-car impacts with the rigid concrete walls of oval tracks. This impressive level of protection was found to be due to the unique combination of a very supportive and tight-fitting cockpit-seating package, a six-point belt restraint system, and effective head padding with an extremely strong chassis that defines the seat and cockpit of a modern Indy car. In 2000 and 2001, a series of fatal crashes in stock car racing created great concern for improving the crash protection for drivers in those racecars. Unlike the Indy car, the typical racing stock car features a more spacious driver cockpit due to its resemblance to the shape of a passenger car. The typical racing seat used in stock cars did not have the same configuration or support characteristics of the Indy car seat, and five-point belt restraints were used. The tubular steel space frame chassis of a stock car also differs from an Indy car's composite chassis structure in both form and mechanical behavior. This paper describes the application of results of the biomechanical analysis of the Indy car crash studies to the unique requirements of stock car racing driver crash protection. Sled test and full-scale crash test data using both Hybrid III frontal crash anthropomorphic test devices (ATDs) and BioSID side crash ATDs for the purpose of evaluating countermeasures involving restraint systems, seats and head/neck restraints has been instrumental in guiding these developments. In addition, the development of deformable walls for oval tracks (the SAFER Barrier) is described as an adjunct to improved occupant restraint through control of the crash forces acting on a racing car. NASCAR (National Association for Stock Car Auto Racing, Inc) implemented crash recording in stock car racing in its three national series in 2002. Data from 2925 crashes from 2002 through the 2005 season are summarized in terms of crash severity, crash direction, injury outcome, and protective system performance.

Injuries in professional motor car racing drivers at a racing circuit between 1996 and 2000.

PubMed

Minoyama, O; Tsuchida, H

2004-10-01

Research on injuries in racing drivers is limited. To gain more information about such injuries. Injuries recorded during and after races between 1996 and 2000 were investigated using the medical charts from the circuit medical centre at Fuji Speedway, which is one of the biggest circuits in Japan. Races were in either single seat/formula cars or saloon cars. Data were obtained from 39 races in single seat cars (1030 participating cars) and 42 races in saloon cars (1577 cars). Fifty injuries were recorded during the single seat car races, and 62 during the saloon car races (injury rate 1.2 per 1000 competitors per race and 0.9 per 1000 competitors per race respectively). Thirteen injuries were recorded after the race, 12 of them in saloon car racing. Bruises were the major injury in single seat car racing (58%). Lower limb bruising was more common than upper limb bruising. Most of the injuries in saloon car racing (53.2%) were neck sprains. The incidence of concussion was high in both groups compared with other high risk sports. There were some differences in injuries between the two types of car. No serious injuries occurred except for one death. However, the driver's body is subjected to large forces in a crash, hence the high incidence of concussion. The injuries recorded after the race emphasise that motor racing is a demanding sport.

Estimation of carotenoid content at the canopy scale using the carotenoid triangle ratio index from in situ and simulated hyperspectral data

NASA Astrophysics Data System (ADS)

Kong, Weiping; Huang, Wenjiang; Zhou, Xianfeng; Song, Xiaoyu; Casa, Raffaele

2016-04-01

Precise estimation of carotenoids (Car) content in plants, from remotely sensed data, is challenging due to their small proportion in the overall total pigment content and to the overlapping of spectral absorption features with chlorophyll (Chl) in the blue region of the spectrum. The use of narrow band vegetation indices (VIs) obtained from hyperspectral data has been considered an effective way to estimate Car content. However, VIs have proved to lack sensitivity to low or high Car content in a number of studies. In this study, the carotenoid triangle ratio index (CTRI), derived from the existing modified triangular vegetation index and a single band reflectance at 531 nm, was proposed and employed to estimate Car canopy content. We tested the potential of three categories of hyperspectral indices earlier proposed for Car, Chl, Car/Chl ratio estimation, and the new CTRI index, for Car canopy content assessment in winter wheat and corn. Spectral reflectance representing plant canopies were simulated using the PROSPECT and SAIL radiative transfer model, with the aim of analyzing saturation effects of these indices, as well as Chl effects on the relationship between spectral indices and Car content. The result showed that the majority of the spectral indices tested, saturated with the increase of Car canopy content above 28 to 64 μg/cm2. Conversely, the CTRI index was more robust and was linearly and highly sensitive to Car content in winter wheat and corn datasets, with coefficients of determination of 0.92 and 0.75, respectively. The corresponding root mean square error of prediction were 6.01 and 9.70 μg/cm2, respectively. Furthermore, the CTRI index did not show a saturation effect and was not greatly influenced by changes of Chl values, outperforming all the other indices tested. Estimation of Car canopy content using the CTRI index provides an insight into diagnosing plant physiological status and environmental stress.

[Research on NEDC ultrafine particle emission characters of a port fuel injection gasoline car].

PubMed

Hu, Zhi-Yuan; Li, Jin; Tan, Pi-Qiang; Lou, Di-Ming

2012-12-01

A Santana gasoline car with multi-port fuel injection (PFI) system was used as the research prototype and an engine exhaust particle sizer (EEPS) was employed to investigate the exhaust ultrafine particle number and size distribution characters of the tested vehicle in new European driving cycle (NEDC). The tested results showed that the vehicle's nuclear particle number, accumulation particle number, as well as the total particle number emission increased when the car drove in accelerated passage, and the vehicle's particle number emission was high during the first 40 seconds after test started and when the speed was over 90 km x h(-1) in extra urban driving cycle (EUDC) in NEDC. The ultrafine particle distribution of the whole NEDC showed a single peak logarithmic distribution, with diameters of the peak particle number emission ranging from 10 nm to 30 nm, and the geometric mean diameter was 24 nm. The ultrafine particle distribution of the urban driving cycle named by the economic commission for Europe (ECE) e. g. ECE I, ECE II - IV, the extra urban driving cycle e. g. EUDC, and the idling, constant speed, acceleration, deceleration operation conditions of NEDC all showed a single peak logarithmic distribution, also with particle diameters of the peak particle number emission ranging from 10 nm to 30 nm, and the geometric mean diameters of different driving cycle and different driving mode were from 14 nm to 42 nm. Therefore, the ultrafine particle emissions of the tested PFI gasoline car were mainly consisted of nuclear mode particles with a diameter of less than 50 nm.

Single-cell multiplexed cytokine profiling of CD19 CAR-T cells reveals a diverse landscape of polyfunctional antigen-specific response.

PubMed

Xue, Qiong; Bettini, Emily; Paczkowski, Patrick; Ng, Colin; Kaiser, Alaina; McConnell, Timothy; Kodrasi, Olja; Quigley, Máire F; Heath, James; Fan, Rong; Mackay, Sean; Dudley, Mark E; Kassim, Sadik H; Zhou, Jing

2017-11-21

It remains challenging to characterize the functional attributes of chimeric antigen receptor (CAR)-engineered T cell product targeting CD19 related to potency and immunotoxicity ex vivo, despite promising in vivo efficacy in patients with B cell malignancies. We employed a single-cell, 16-plex cytokine microfluidics device and new analysis techniques to evaluate the functional profile of CD19 CAR-T cells upon antigen-specific stimulation. CAR-T cells were manufactured from human PBMCs transfected with the lentivirus encoding the CD19-BB-z transgene and expanded with anti-CD3/anti-CD28 coated beads. The enriched CAR-T cells were stimulated with anti-CAR or control IgG beads, stained with anti-CD4 RPE and anti-CD8 Alexa Fluor 647 antibodies, and incubated for 16 h in a single-cell barcode chip (SCBC). Each SCBC contains ~12,000 microchambers, covered with a glass slide that was pre-patterned with a complete copy of a 16-plex antibody array. Protein secretions from single CAR-T cells were captured and subsequently analyzed using proprietary software and new visualization methods. We demonstrate a new method for single-cell profiling of CD19 CAR-T pre-infusion products prepared from 4 healthy donors. CAR-T single cells exhibited a marked heterogeneity of cytokine secretions and polyfunctional (2+ cytokine) subsets specific to anti-CAR bead stimulation. The breadth of responses includes anti-tumor effector (Granzyme B, IFN-γ, MIP-1α, TNF-α), stimulatory (GM-CSF, IL-2, IL-8), regulatory (IL-4, IL-13, IL-22), and inflammatory (IL-6, IL-17A) functions. Furthermore, we developed two new bioinformatics tools for more effective polyfunctional subset visualization and comparison between donors. Single-cell, multiplexed, proteomic profiling of CD19 CAR-T product reveals a diverse landscape of immune effector response of CD19 CAR-T cells to antigen-specific challenge, providing a new platform for capturing CAR-T product data for correlative analysis. Additionally, such high dimensional data requires new visualization methods to further define precise polyfunctional response differences in these products. The presented biomarker capture and analysis system provides a more sensitive and comprehensive functional assessment of CAR-T pre-infusion products and may provide insights into the safety and efficacy of CAR-T cell therapy.

Injuries in professional motor car racing drivers at a racing circuit between 1996 and 2000

PubMed Central

Minoyama, O; Tsuchida, H

2004-01-01

Background: Research on injuries in racing drivers is limited. Objective: To gain more information about such injuries. Methods: Injuries recorded during and after races between 1996 and 2000 were investigated using the medical charts from the circuit medical centre at Fuji Speedway, which is one of the biggest circuits in Japan. Races were in either single seat/formula cars or saloon cars. Results: Data were obtained from 39 races in single seat cars (1030 participating cars) and 42 races in saloon cars (1577 cars). Fifty injuries were recorded during the single seat car races, and 62 during the saloon car races (injury rate 1.2 per 1000 competitors per race and 0.9 per 1000 competitors per race respectively). Thirteen injuries were recorded after the race, 12 of them in saloon car racing. Bruises were the major injury in single seat car racing (58%). Lower limb bruising was more common than upper limb bruising. Most of the injuries in saloon car racing (53.2%) were neck sprains. The incidence of concussion was high in both groups compared with other high risk sports. Conclusions: There were some differences in injuries between the two types of car. No serious injuries occurred except for one death. However, the driver's body is subjected to large forces in a crash, hence the high incidence of concussion. The injuries recorded after the race emphasise that motor racing is a demanding sport. PMID:15388550

CAR-T cells are serial killers

PubMed Central

Davenport, Alexander J; Jenkins, Misty R; Ritchie, David S; Prince, H Miles; Trapani, Joseph A; Kershaw, Michael H; Darcy, Phillip K; Neeson, Paul J

2015-01-01

Chimeric antigen receptor (CAR) T cells have enjoyed unprecedented clinical success against haematological malignancies in recent years. However, several aspects of CAR T cell biology remain unknown. We recently compared CAR and T cell receptor (TCR)-based killing in the same effector cell and showed that CAR T cells can not only efficiently kill single tumor targets, they can also kill multiple tumor targets in a sequential manner. Single and serial killing events were not sustained long term due to CAR down-regulation after 20 hours. PMID:26587330

CAR-T cells are serial killers.

PubMed

Davenport, Alexander J; Jenkins, Misty R; Ritchie, David S; Prince, H Miles; Trapani, Joseph A; Kershaw, Michael H; Darcy, Phillip K; Neeson, Paul J

2015-12-01

Chimeric antigen receptor (CAR) T cells have enjoyed unprecedented clinical success against haematological malignancies in recent years. However, several aspects of CAR T cell biology remain unknown. We recently compared CAR and T cell receptor (TCR)-based killing in the same effector cell and showed that CAR T cells can not only efficiently kill single tumor targets, they can also kill multiple tumor targets in a sequential manner. Single and serial killing events were not sustained long term due to CAR down-regulation after 20 hours.

49 CFR 238.113 - Emergency window exits.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Each single-level passenger car shall have a minimum of four emergency... car, in a staggered configuration where practical. (See Figure 1 to this subpart; see also Figures 1b...

49 CFR 238.113 - Emergency window exits.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Each single-level passenger car shall have a minimum of four emergency... car, in a staggered configuration where practical. (See Figure 1 to this subpart; see also Figures 1b...

49 CFR 238.113 - Emergency window exits.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Each single-level passenger car shall have a minimum of four emergency... car, in a staggered configuration where practical. (See Figure 1 to this subpart; see also Figures 1b...

49 CFR 238.113 - Emergency window exits.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Each single-level passenger car shall have a minimum of four emergency... car, in a staggered configuration where practical. (See Figure 1 to this subpart; see also Figures 1b...

49 CFR 238.113 - Emergency window exits.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Each single-level passenger car shall have a minimum of four emergency... car, in a staggered configuration where practical. (See Figure 1 to this subpart; see also Figures 1b...

Analysis of injury severity of drivers involved in single- and two-vehicle crashes on highways in Ontario.

PubMed

Lee, Chris; Li, Xuancheng

2014-10-01

This study analyzes driver's injury severity in single- and two-vehicle crashes and compares the effects of explanatory variables among various types of crashes. The study identified factors affecting injury severity and their effects on severity levels using 5-year crash records for provincial highways in Ontario, Canada. Considering heteroscedasticity in the effects of explanatory variables on injury severity, the heteroscedastic ordered logit (HOL) models were developed for single- and two-vehicle crashes separately. The results of the models show that there exists heteroscedasticity for young drivers (≤30), safety equipment and ejection in the single-vehicle crash model, and female drivers, safety equipment and head-on collision in the two-vehicle crash models. The results also show that young car drivers have opposite effects between single-car and car-car crashes, and sideswipe crashes have opposite effects between car-car and truck-truck crashes. The study demonstrates that separate HOL models for single-vehicle and different types of two-vehicle crashes can identify differential effects of factors on driver's injury severity. Copyright © 2014 Elsevier Ltd. All rights reserved.

Clustered atom-replaced structure in single-crystal-like metal oxide

NASA Astrophysics Data System (ADS)

Araki, Takeshi; Hayashi, Mariko; Ishii, Hirotaka; Yokoe, Daisaku; Yoshida, Ryuji; Kato, Takeharu; Nishijima, Gen; Matsumoto, Akiyoshi

2018-06-01

By means of metal organic deposition using trifluoroacetates (TFA-MOD), we replaced and localized two or more atoms in a single-crystalline structure having almost perfect orientation. Thus, we created a new functional structure, namely, clustered atom-replaced structure (CARS), having single-crystal-like metal oxide. We replaced metals in the oxide with Sm and Lu and localized them. Energy dispersive x-ray spectroscopy results, where the Sm signal increases with the Lu signal in the single-crystalline structure, confirm evidence of CARS. We also form other CARS with three additional metals, including Pr. The valence number of Pr might change from 3+ to approximately 4+, thereby reducing the Pr–Ba distance. We directly observed the structure by a high-angle annular dark-field image, which provided further evidence of CARS. The key to establishing CARS is an equilibrium chemical reaction and a combination of additional larger and smaller unit cells to matrix cells. We made a new functional metal oxide with CARS and expect to realize CARS in other metal oxide structures in the future by using the above-mentioned process.

Differential item functioning analysis of the Vanderbilt Expertise Test for cars.

PubMed

Lee, Woo-Yeol; Cho, Sun-Joo; McGugin, Rankin W; Van Gulick, Ana Beth; Gauthier, Isabel

2015-01-01

The Vanderbilt Expertise Test for cars (VETcar) is a test of visual learning for contemporary car models. We used item response theory to assess the VETcar and in particular used differential item functioning (DIF) analysis to ask if the test functions the same way in laboratory versus online settings and for different groups based on age and gender. An exploratory factor analysis found evidence of multidimensionality in the VETcar, although a single dimension was deemed sufficient to capture the recognition ability measured by the test. We selected a unidimensional three-parameter logistic item response model to examine item characteristics and subject abilities. The VETcar had satisfactory internal consistency. A substantial number of items showed DIF at a medium effect size for test setting and for age group, whereas gender DIF was negligible. Because online subjects were on average older than those tested in the lab, we focused on the age groups to conduct a multigroup item response theory analysis. This revealed that most items on the test favored the younger group. DIF could be more the rule than the exception when measuring performance with familiar object categories, therefore posing a challenge for the measurement of either domain-general visual abilities or category-specific knowledge.

Design of an intelligent car

NASA Astrophysics Data System (ADS)

Na, Yongyi

2017-03-01

The design of simple intelligent car, using AT89S52 single chip microcomputer as the car detection and control core; The metal sensor TL - Q5MC induction to iron, to detect the way to send feedback to the signal of single chip microcomputer, make SCM according to the scheduled work mode to control the car in the area according to the predetermined speed, and the operation mode of the microcontroller choose different also can control the car driving along s-shaped iron; Use A44E hall element to detect the car speeds; Adopts 1602 LCD display time of car driving, driving the car to stop, take turns to show the car driving time, distance, average speed and the speed of time. This design has simple structure and is easy to implement, but are highly intelligent, humane, to a certain extent reflects the intelligence.

Pressure Effects on Worn Passenger Car Tire Carcasses

DOT National Transportation Integrated Search

1975-02-01

Work is described to examine the value of hydrostatic proof pressure testing in selecting used tire carcasses for retreading. Preliminary experiments on single tire cords indicate that overloads close to rupture do not damage subsequent fatigue life....

Coherent Anti-stokes Raman Spectroscopy (CARS) of gun propellant flames

NASA Technical Reports Server (NTRS)

Mcilwain, M. E.; Harris, L. E.

1980-01-01

Temperature measurements were made in a slightly fuel rich, premixed propane/air reference flame and nitrate ester propellant flames burning in air at atmospheric pressure using coherent anti-stokes raman scattering (CARS). Both single and multiple pulse VARS spectra of nitrogen in the reference flame were in good agreement with calculated and reported values. Single pulse CARS nitrogen spectra obtained in the propellant flames were analyzed to give temperatures consistent with values calculated using the NASA-Lewis thermochemical calculation. Comparison of a 0.1 second separated sequence of single pulse CARS spectra indicate turbulent air mixing in these propellant flames. The CARS spectral results demonstrate that temporal and spatially resolved temperature measurements could be determined in transient, turbulent flames.

The European New Car Assessment Programme: A historical review.

PubMed

van Ratingen, Michiel; Williams, Aled; Lie, Anders; Seeck, Andre; Castaing, Pierre; Kolke, Reinhard; Adriaenssens, Guido; Miller, Andrew

2016-04-01

Established in 1997, the European New Car Assessment Programme (Euro NCAP) provides consumers with a safety performance assessment for the majority of the most popular cars in Europe. Thanks to its rigorous crash tests, Euro NCAP has rapidly become an important driver safety improvement to new cars. After ten years of rating vehicles, Euro NCAP felt that a change was necessary to stay in tune with rapidly emerging driver assistance and crash avoidance systems and to respond to shifting priorities in road safety. A new overall rating system was introduced that combines the most important aspects of vehicle safety under a single star rating. The overall rating system has allowed Euro NCAP to continue to push for better fitment and higher performance for vehicles sold on the European market. In the coming years, the safety rating is expected to play an important role in the support of the roll-out of highly automated vehicles.

Differential item functioning analysis of the Vanderbilt Expertise Test for cars

PubMed Central

Lee, Woo-Yeol; Cho, Sun-Joo; McGugin, Rankin W.; Van Gulick, Ana Beth; Gauthier, Isabel

2015-01-01

The Vanderbilt Expertise Test for cars (VETcar) is a test of visual learning for contemporary car models. We used item response theory to assess the VETcar and in particular used differential item functioning (DIF) analysis to ask if the test functions the same way in laboratory versus online settings and for different groups based on age and gender. An exploratory factor analysis found evidence of multidimensionality in the VETcar, although a single dimension was deemed sufficient to capture the recognition ability measured by the test. We selected a unidimensional three-parameter logistic item response model to examine item characteristics and subject abilities. The VETcar had satisfactory internal consistency. A substantial number of items showed DIF at a medium effect size for test setting and for age group, whereas gender DIF was negligible. Because online subjects were on average older than those tested in the lab, we focused on the age groups to conduct a multigroup item response theory analysis. This revealed that most items on the test favored the younger group. DIF could be more the rule than the exception when measuring performance with familiar object categories, therefore posing a challenge for the measurement of either domain-general visual abilities or category-specific knowledge. PMID:26418499

Selected topics in railroad tank car safety. Volume 2 : test plan for accelerated life testing of thermally shielded tank cars

DOT National Transportation Integrated Search

1978-08-01

A test plan for the accelerated life testing of thermally shielded tank cars is described. The test program would be conducted at the DOT Transportation Test Center in Pueblo, Colorado. Eighteen tank cars would be included in the program. Five cars w...

Determination of car on-road black carbon and particle number emission factors and comparison between mobile and stationary measurements

NASA Astrophysics Data System (ADS)

Ježek, I.; Drinovec, L.; Ferrero, L.; Carriero, M.; Močnik, G.

2015-01-01

We have used two methods for measuring emission factors (EFs) in real driving conditions on five cars in a controlled environment: the stationary method, where the investigated vehicle drives by the stationary measurement platform and the composition of the plume is measured, and the chasing method, where a mobile measurement platform drives behind the investigated vehicle. We measured EFs of black carbon and particle number concentration. The stationary method was tested for repeatability at different speeds and on a slope. The chasing method was tested on a test track and compared to the portable emission measurement system. We further developed the data processing algorithm for both methods, trying to improve consistency, determine the plume duration, limit the background influence and facilitate automatic processing of measurements. The comparison of emission factors determined by the two methods showed good agreement. EFs of a single car measured with either method have a specific distribution with a characteristic value and a long tail of super emissions. Measuring EFs at different speeds or slopes did not significantly influence the EFs of different cars; hence, we propose a new description of vehicle emissions that is not related to kinematic or engine parameters, and we rather describe the vehicle EF with a characteristic value and a super emission tail.

Multiplex CARS imaging with spectral notch shaped laser pulses delivered by optical fibers.

PubMed

Oh, Seung Ryeol; Park, Joo Hyun; Kim, Kyung-Soo; Lee, Jae Yong; Kim, Soohyun

2017-12-11

We present an experimental demonstration of single-pulse coherent anti-Stokes Raman spectroscopy (CARS) using a spectrally shaped broadband laser that is delivered by an optical fiber to a sample at its distal end. The optical fiber consists of a fiber Bragg grating component to serve as a narrowband notch filter and a combined large-mode-area fiber to transmit such shaped ultrashort laser pulses without spectral distortion in a long distance. Experimentally, our implementation showed a capability to measure CARS spectra of various samples with molecular vibrations in the fingerprint region. Furthermore, CARS imaging of poly(methyl methacrylate) bead samples was carried out successfully under epi-CARS geometry in which backward-scattered CARS signals were collected into a multimode optical fiber. A compatibility of single-pulse CARS scheme with fiber optics, verified in this study, implies a potential for future realization of compact all-fiber CARS spectroscopic imaging systems.

49 CFR 238.114 - Rescue access windows.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Except as provided in this paragraph (a)(1) and in paragraphs (a)(1)(i), (a)(1)(ii), and (a)(5) of this section, each single-level passenger car shall have a minimum of two...

49 CFR 238.114 - Rescue access windows.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Except as provided in this paragraph (a)(1) and in paragraphs (a)(1)(i), (a)(1)(ii), and (a)(5) of this section, each single-level passenger car shall have a minimum of two...

49 CFR 238.114 - Rescue access windows.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Except as provided in this paragraph (a)(1) and in paragraphs (a)(1)(i), (a)(1)(ii), and (a)(5) of this section, each single-level passenger car shall have a minimum of two...

49 CFR 238.114 - Rescue access windows.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Except as provided in this paragraph (a)(1) and in paragraphs (a)(1)(i), (a)(1)(ii), and (a)(5) of this section, each single-level passenger car shall have a minimum of two...

49 CFR 238.114 - Rescue access windows.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., electrical locker, or kitchen); and (B) There are no more than eight seats in the seating area. (4) Cars with...) Single-level passenger cars. Except as provided in this paragraph (a)(1) and in paragraphs (a)(1)(i), (a)(1)(ii), and (a)(5) of this section, each single-level passenger car shall have a minimum of two...

Intelligent transportation systems field operational test cross-cutting study : commercial vehicle administrative processes

DOT National Transportation Integrated Search

1999-10-01

This analysis brief explores differences and similarities among the national crash experience of combination-unit trucks (CUTs), single-unit trucks (SUTs), and "all vehicles" (principally cars and light truck/vans). These CUT vs. SUT vs. all vehicle ...

Is passenger vehicle incompatibility still a problem?

PubMed

Teoh, Eric R; Nolan, Joseph M

2012-01-01

Passenger cars often are at a disadvantage when colliding with light trucks (sport utility vehicles [SUVs] and pickups) due to differences in mass, vehicle structural alignment, and stiffness. In 2003, vehicle manufacturers agreed to voluntary measures to improve compatibility, especially in front-to-front and front-to-side crashes, with full adherence to be achieved by September 2009. This study examined whether fatality rates are consistent with the expected benefit of this agreement. Analyses examined 2 death rates for 1- to 4-year-old passenger vehicles during 2000-2001 and 2008-2009 in the United States: occupant deaths per million registered vehicle years in these vehicles and deaths in other cars that collided with these vehicles in 2-vehicle crashes per million registered vehicle years. These rates were computed for each study period and for cars/minivans (referred to as cars), SUVs, and pickups by curb weight (in 500-pound increments). The latter death rate, referred to as the car crash partner death rate, also was computed for front-to-front crashes and front-to-side crashes where the front of the 1- to 4-year-old vehicle struck the side of the partner car. In both study periods, occupant death rates generally decreased for each vehicle type both with increasing curb weight and over time. SUVs experienced the greatest declines compared with cars and pickups. This is due in part to the early fitment of electronic stability control in SUVs, which drastically reduced the incidence of single-vehicle rollover crashes. Pickups had the highest death rates in both study periods. Car crash partner death rates generally declined over time for all vehicle categories but more steeply for SUVs and pickups colliding with cars than for cars colliding with cars. In fact, the car crash partner death rates for SUVs and cars were nearly identical during 2008-2009, suggesting that the voluntary design changes for compatibility have been effective. Car crash partner death rates also declined for pickups, but their rates were consistently the highest in both study periods. It is impossible to disentangle the individual contributions of the compatibility agreement, improved crashworthiness of cars, and other factors in reducing car crash partner fatality rates. However, the generally larger reductions in car crash partner death rates for SUVs and pickups indicate the likely benefits of the agreement. Overall, this study finds that the system of regulatory testing, voluntary industry initiatives, and consumer information testing has led to a passenger vehicle fleet that is much more compatible in crashes.

Nonviral RNA transfection to transiently modify T cells with chimeric antigen receptors for adoptive therapy.

PubMed

Riet, Tobias; Holzinger, Astrid; Dörrie, Jan; Schaft, Niels; Schuler, Gerold; Abken, Hinrich

2013-01-01

Redirecting T cells with a chimeric antigen receptor (CAR) of predefined specificity showed remarkable efficacy in the adoptive therapy trials of malignant diseases. The CAR consists of a single chain fragment of variable region (scFv) antibody targeting domain covalently linked to the CD3ζ signalling domain of the T cell receptor complex to mediate T cell activation upon antigen engagement. By using an antibody-derived targeting domain a CAR can potentially redirect T cells towards any target expressed on the cell surface as long as a binding domain is available. Antibody-mediated targeting moreover circumvents MHC restriction of the targeted antigen, thereby broadening the potential of applicability of adoptive T cell therapy. While T cells were so far genetically modified by viral transduction, transient modification with a CAR by RNA transfection gained increasing interest during the last years. This chapter focuses on methods to modify human T cells from peripheral blood with a CAR by electroporation of in vitro transcribed RNA and to test modified T cells for function for use in adoptive immunotherapy.

49 CFR 213.345 - Vehicle qualification testing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... a lateral accelerometer mounted on the car floor, shall be limited to no greater than 0.3g single...) shall be limited to no greater than 0.604, where L may not exceed 0.3g and V may not exceed 0.55g. (c...

49 CFR 213.345 - Vehicle qualification testing.

Code of Federal Regulations, 2011 CFR

2011-10-01

... a lateral accelerometer mounted on the car floor, shall be limited to no greater than 0.3g single...) shall be limited to no greater than 0.604, where L may not exceed 0.3g and V may not exceed 0.55g. (c...

Monitoring system of hydraulic lifting device based on the fiber optic sensors

NASA Astrophysics Data System (ADS)

Fajkus, Marcel; Nedoma, Jan; Novak, Martin; Martinek, Radek; Vanus, Jan; Mec, Pavel; Vasinek, Vladimir

2017-10-01

This article deals with the description of the monitoring system of hydraulic lifting device based on the fiber-optic sensors. For minimize the financial costs of the proposed monitoring system, the power evaluation of measured signal has been chosen. The solution is based on an evaluation of the signal obtained using the single point optic fiber sensors with overlapping reflective spectra. For encapsulation of the sensors was used polydimethylsiloxane (PDMS) polymer. To obtain a information of loading is uses the action of deformation of the lifting device on the pair single point optic fiber sensors mounted on the lifting device of the tested car. According to the proposed algorithm is determined information of pressure with an accuracy of +/- 5 %. Verification of the proposed system was realized on the various types of the tested car with different loading. The original contribution of the paper is to verify the new low-cost system for monitoring the hydraulic lifting device based on the fiber-optic sensors.

Stand for testing the electrical race car engine

NASA Astrophysics Data System (ADS)

Baier, M.; Franiasz, J.; Mierzwa, P.; Wylenzek, D.

2015-11-01

An engine test stand created especially for research of electrical race car is described in the paper. The car is an aim of Silesian Greenpower project whose participants build and test electrical vehicles to take part in international races in Great Britain. The engine test stand is used to test and measure the characteristics of vehicles and their engines. It has been designed particularly to test the electric cars engineered by students of Silesian Greenpower project. The article contains a description how the test stand works and shows its versatility in many areas. The paper presents both construction of the test stand, control system and sample results of conducted research. The engine test stand was designed and modified using PLM Siemens NX 8.5. The construction of the test stand is highly modular, which means it can be used both for testing the vehicle itself or for tests without the vehicle. The test stand has its own wheel, motor, powertrain and braking system with second engine. Such solution enables verifying various concepts without changing the construction of the vehicle. The control system and measurement system are realized by enabling National Instruments product myRIO (RIO - Reconfigurable Input/Output). This controller in combination with powerful LabVIEW environment performs as an advanced tool to control torque and speed simultaneously. It is crucial as far as the test stand is equipped in two motors - the one being tested and the braking one. The feedback loop is realized by an optical encoder cooperating with the rotor mounted on the wheel. The results of tests are shown live on the screen both as a chart and as single values. After performing several tests there is a report generated. The engine test stand is widely used during process of the Silesian Greenpower vehicle design. Its versatility enables powertrain testing, wheels and tires tests, thermal analysis and more.

Development and validation of a Kalman filter-based model for vehicle slip angle estimation

NASA Astrophysics Data System (ADS)

Gadola, M.; Chindamo, D.; Romano, M.; Padula, F.

2014-01-01

It is well known that vehicle slip angle is one of the most difficult parameters to measure on a vehicle during testing or racing activities. Moreover, the appropriate sensor is very expensive and it is often difficult to fit to a car, especially on race cars. We propose here a strategy to eliminate the need for this sensor by using a mathematical tool which gives a good estimation of the vehicle slip angle. A single-track car model, coupled with an extended Kalman filter, was used in order to achieve the result. Moreover, a tuning procedure is proposed that takes into consideration both nonlinear and saturation characteristics typical of vehicle lateral dynamics. The effectiveness of the proposed algorithm has been proven by both simulation results and real-world data.

Development and field performance of indy race car head impact padding.

PubMed

Melvin, J W; Bock, H; Anderson, K; Gideon, T

2001-11-01

The close-fitting cockpit of the modern Indy car single seat race car has the potential to provide a high level of head and neck impact protection in rear and side impacts. Crash investigation has shown that a wide variety of materials have been used as the padding for these cockpits and, as a result, produced varying outcomes in crashes. Additionally, these pads have not always been positioned for optimal performance. The purpose of this study was to investigate the head impact performance of a variety of energy-absorbing padding materials under impact conditions typical of Indy car rear impacts and to identify superior materials and methods of improving their performance as race car head pads. An extensive series of tests with the helmeted Hybrid III test dummy head and neck on an impact mini-sled was conducted to explore head padding concepts. Following this, a performance specification for a simplified impact test using a rigid headform that simulates the helmeted head was developed and recommendations for performance levels of head padding based on biomechanical data on helmeted head impacts were made. In 1997, during the time that the head pad research was being performed, the Indy Racing League introduced a new chassis specification for their cars. There were a number of rear- and side-impact crashes during that season that resulted in seven severe head injuries. Examples of the head padding in those cars were included in the experimental study. The results of the head pad research were used to specify new padding materials that met the new biomechanical criteria. The placement of the head pads was also changed for better location of the padding. These changes instituted in 1998 have reduced the number of head injuries in crashes similar to or more severe than those of 1997 and have resulted in only occasional moderate head injuries (concussions) in the 1998 and 1999 seasons.

Evolution of the crashworthiness and aggressivity of the Spanish car fleet.

PubMed

Gómez Méndez, Alvaro; Aparicio Izquierdo, Francisco; Arenas Ramírez, Blanca

2010-11-01

This paper investigates the relationship between a passenger car's year of registration and its crashworthiness and aggressivity in real-world crashes. Crashworthiness is defined as the ability of a car to protect its own occupants, and has been evaluated in single and two-car crashes. Aggressivity is defined as the ability to protect users travelling in other vehicles, and has been evaluated only in two-car crashes. The dependent variable is defined as the proportion of injured drivers who are killed or seriously injured; following previous research, we refer to this magnitude as injury severity. A decrease in the injury severity of a driver is interpreted as an improvement in the crashworthiness of their car; similarly, a decrease in the injury severity of the opponent driver is regarded as an improvement in aggressivity. Data have been extracted from the Spanish Road Accident Database, which contains information on every accident registered by the police in which at least one person was injured. Two types of regression models have been used: logistic regression models in single-car crashes, and generalised estimating equations (GEE) models in two-car crashes. GEE allow to take account of the correlation between the injury severities of drivers involved in the same crash. The independent variables considered have been: year of registration of the subject car (crashworthiness component), year of registration of the opponent car (aggressivity component), and several factors related to road, driver and environment. Our models confirm that crashworthiness has largely improved in two-car crashes: when crashing into the average opponent car, drivers of cars registered before 1985 have a significantly higher probability of being killed or seriously injured than drivers of cars registered in 2000-2005 (odds ratio: 1.80; 95% confidence interval: 1.61; 2.01). In single-car crashes, the improvement in crashworthiness is very slight (odds ratio: 1.04; 95% confidence interval: 0.93; 1.16). On the other hand, we have also found a significant worsening in aggressivity in two-car crashes: the driver of the average car has a significantly lower probability of being killed or seriously injured when crashing into a car registered before 1985, than when crashing into a car registered in 2000-2005 (odds ratio: 0.52; 95% confidence interval: 0.45; 0.60). Our results are consistent with a large amount of previous research that has reported significant improvements in the protection of car occupants. They also add to some recent studies that have found a worsening in the aggressivity of modern cars. This trend may be reflecting the impact of differences in masses and travel speeds, as well as the influence of consumer choices. The precise reasons have to be investigated. Also, the causes have to be found for so large a discrepancy between crashworthiness in single and two-car crashes. 2010 Elsevier Ltd. All rights reserved.

Application of Coherent Anti-Stokes Raman Scattering to Combustion Media.

DTIC Science & Technology

1981-02-01

BANDS FOR REAL-TIME TEMPERATURE MEASUREMENT IN FLAMES 44 3.6 COMPARISONS OF SINGLE-SHOT THERMOMETRY OF CARS WITH OTHER OPTICAL THERMOMETRIC ...b COMPARISONS OF SINGLE-SHOT THERMOMETRY OF CARS WITH OTHER OPTICAL THERMOMETRIC TECHNIQUES Two-Line Fluorescence A fluorescence system was developed...constitute a firm basis for evaluating the validity and accuracy of the CARS process as a thermometric tool for flames. Winefordner 30 has shown that the

Individual motile CD4+ T cells can participate in efficient multi-killing through conjugation to multiple tumor cells

PubMed Central

Liadi, Ivan; Singh, Harjeet; Romain, Gabrielle; Rey-Villamizar, Nicolas; Merouane, Amine; Adolacion, Jay R T.; Kebriaei, Partow; Huls, Helen; Qiu, Peng; Roysam, Badrinath; Cooper, Laurence J.N.; Varadarajan, Navin

2015-01-01

T cells genetically modified to express a CD19-specific chimeric antigen receptor (CAR) for the investigational treatment of B-cell malignancies comprise a heterogeneous population, and their ability to persist and participate in serial killing of tumor cells is a predictor of therapeutic success. We implemented Timelapse Imaging Microscopy In Nanowell Grids (TIMING) to provide direct evidence that CD4+CAR+ T cells (CAR4 cells) can engage in multi-killing via simultaneous conjugation to multiple tumor cells. Comparisons of the CAR4 cells and CD8+CAR+ T cells (CAR8 cells) demonstrate that while CAR4 cells can participate in killing and multi-killing, they do so at slower rates, likely due to the lower Granzyme B content. Significantly, in both sets of T cells, a minor sub-population of individual T cells identified by their high motility, demonstrated efficient killing of single tumor cells. By comparing both the multi-killer and single killer CAR+ T cells it appears that the propensity and kinetics of T-cell apoptosis was modulated by the number of functional conjugations. T cells underwent rapid apoptosis, and at higher frequencies, when conjugated to single tumor cells in isolation and this effect was more pronounced on CAR8 cells. Our results suggest that the ability of CAR+ T cells to participate in multi-killing should be evaluated in the context of their ability to resist activation induced cell death (AICD). We anticipate that TIMING may be utilized to rapidly determine the potency of T-cell populations and may facilitate the design and manufacture of next-generation CAR+ T cells with improved efficacy. PMID:25711538

An investigation into NVC characteristics of vehicle behaviour using modal analysis

NASA Astrophysics Data System (ADS)

Hanouf, Zahir; Faris, Waleed F.; Ahmad, Kartini

2017-03-01

NVC characterizations of vehicle behavior is one essential part of the development targets in automotive industries. Therefore understanding dynamic behavior of each structural part of the vehicle is a major requirement in improving the NVC characteristics of a vehicle. The main focus of this research is to investigate structural dynamic behavior of a passenger car using modal analysis part by part technique and apply this method to derive the interior noise sources. In the first part of this work computational modal analysis part by part tests were carried out to identify the dynamic parameters of the passenger car. Finite elements models of the different parts of the car are constructed using VPG 3.2 software. Ls-Dyna pre and post processing was used to identify and analyze the dynamic behavior of each car components panels. These tests had successfully produced natural frequencies and their associated mode shapes of such panels like trunk, hood, roof and door panels. In the second part of this research, experimental modal analysis part by part is performed on the selected car panels to extract modal parameters namely frequencies and mode shapes. The study establishes the step-by-step procedures to carry out experimental modal analysis on the car structures, using single input excitation and multi-output responses (SIMO) technique. To ensure the validity of the results obtained by the previous method an inverse method was done by fixing the response and moving the excitation and the results found were absolutely the same. Finally, comparison between results obtained from both analyses showed good similarity in both frequencies and mode shapes. Conclusion drawn from this part of study was that modal analysis part-by-part can be strongly used to establish the dynamic characteristics of the whole car. Furthermore, the developed method is also can be used to show the relationship between structural vibration of the car panels and the passengers’ noise comfort inside the cabin.

49 CFR 210.33 - Operation standards (switcher locomotives, load cell test stands, car coupling operations, and...

Code of Federal Regulations, 2010 CFR

2010-10-01

... cell test stands, car coupling operations, and retarders). 210.33 Section 210.33 Transportation Other... (switcher locomotives, load cell test stands, car coupling operations, and retarders). (a) Measurement on receiving property of the noise emission levels from switcher locomotives, load cell test stands, car...

49 CFR 210.33 - Operation standards (switcher locomotives, load cell test stands, car coupling operations, and...

Code of Federal Regulations, 2013 CFR

2013-10-01

... cell test stands, car coupling operations, and retarders). 210.33 Section 210.33 Transportation Other... (switcher locomotives, load cell test stands, car coupling operations, and retarders). (a) Measurement on receiving property of the noise emission levels from switcher locomotives, load cell test stands, car...

49 CFR 210.33 - Operation standards (switcher locomotives, load cell test stands, car coupling operations, and...

Code of Federal Regulations, 2012 CFR

2012-10-01

... cell test stands, car coupling operations, and retarders). 210.33 Section 210.33 Transportation Other... (switcher locomotives, load cell test stands, car coupling operations, and retarders). (a) Measurement on receiving property of the noise emission levels from switcher locomotives, load cell test stands, car...

49 CFR 210.33 - Operation standards (switcher locomotives, load cell test stands, car coupling operations, and...

Code of Federal Regulations, 2014 CFR

2014-10-01

... cell test stands, car coupling operations, and retarders). 210.33 Section 210.33 Transportation Other... (switcher locomotives, load cell test stands, car coupling operations, and retarders). (a) Measurement on receiving property of the noise emission levels from switcher locomotives, load cell test stands, car...

49 CFR 210.33 - Operation standards (switcher locomotives, load cell test stands, car coupling operations, and...

Code of Federal Regulations, 2011 CFR

2011-10-01

... cell test stands, car coupling operations, and retarders). 210.33 Section 210.33 Transportation Other... (switcher locomotives, load cell test stands, car coupling operations, and retarders). (a) Measurement on receiving property of the noise emission levels from switcher locomotives, load cell test stands, car...

A collision dynamics model of a multi-level train

DOT National Transportation Integrated Search

2006-11-05

In train collisions, multi-level rail passenger vehicles can deform in modes that are different from the behavior of single level cars. The deformation in single level cars usually occurs at the front end during a collision. In one particular inciden...

Tandem CAR T cells targeting HER2 and IL13Rα2 mitigate tumor antigen escape

PubMed Central

Mukherjee, Malini; Grada, Zakaria; Pignata, Antonella; Landi, Daniel; Navai, Shoba A.; Wakefield, Amanda; Bielamowicz, Kevin; Chow, Kevin K.H.; Brawley, Vita S.; Byrd, Tiara T.; Krebs, Simone; Gottschalk, Stephen; Wels, Winfried S.; Baker, Matthew L.; Dotti, Gianpietro; Mamonkin, Maksim; Brenner, Malcolm K.

2016-01-01

In preclinical models of glioblastoma, antigen escape variants can lead to tumor recurrence after treatment with CAR T cells that are redirected to single tumor antigens. Given the heterogeneous expression of antigens on glioblastomas, we hypothesized that a bispecific CAR molecule would mitigate antigen escape and improve the antitumor activity of T cells. Here, we created a CAR that joins a HER2-binding scFv and an IL13Rα2-binding IL-13 mutein to make a tandem CAR exodomain (TanCAR) and a CD28.ζ endodomain. We determined that patient TanCAR T cells showed distinct binding to HER2 or IL13Rα2 and had the capability to lyse autologous glioblastoma. TanCAR T cells exhibited activation dynamics that were comparable to those of single CAR T cells upon encounter of HER2 or IL13Rα2. We observed that TanCARs engaged HER2 and IL13Rα2 simultaneously by inducing HER2-IL13Rα2 heterodimers, which promoted superadditive T cell activation when both antigens were encountered concurrently. TanCAR T cell activity was more sustained but not more exhaustible than that of T cells that coexpressed a HER2 CAR and an IL13Rα2 CAR, T cells with a unispecific CAR, or a pooled product. In a murine glioblastoma model, TanCAR T cells mitigated antigen escape, displayed enhanced antitumor efficacy, and improved animal survival. Thus, TanCAR T cells show therapeutic potential to improve glioblastoma control by coengaging HER2 and IL13Rα2 in an augmented, bivalent immune synapse that enhances T cell functionality and reduces antigen escape. PMID:27427982

The Influences of Arm Resist Motion on a CAR Crash Test Using Hybrid III Dummy with Human-Like Arm

NASA Astrophysics Data System (ADS)

Kim, Yongchul; Youm, Youngil; Bae, Hanil; Choi, Hyeonki

Safety of the occupant during the crash is very essential design element. Many researches have been investigated in reducing the fatal injury of occupant. They are focusing on the development of a dummy in order to obtain the real human-like motion. However, they have not considered the arm resist motion during the car accident. In this study, we would like to suggest the importance of the reactive force of the arm in a car crash. The influences of reactive force acting on the human upper extremity were investigated using the impedance experimental method with lumped mass model of hand system and a Hybrid III dummy with human-like arm. Impedance parameters (e.g. inertia, spring constant and damping coefficient) of the elbow joint in maximum activation level were measured by free oscillation test using single axis robot. The results showed that without seat belt, the reactive force of human arm reduced the head, chest, and femur injury, and the flexion moment of the neck is higher than that of the conventional dummy.

Analysis of accelerations measured during full-scale tank car impact tests

DOT National Transportation Integrated Search

2007-04-01

Tank car impact responses were investigated using accelerometers mounted at various locations on a tank car. Several tests were run with both a full and an empty tank car, and varying the tank car impact speed. The data from the accelerometers went t...

49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

Code of Federal Regulations, 2011 CFR

2011-10-01

... specification tank cars. 180.509 Section 180.509 Transportation Other Regulations Relating to Transportation...) CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.509 Requirements for inspection and test of specification tank cars. (a) General. (1) Each tank car facility shall...

Crippling load test of Budd Pioneer Car 244, test 3.

DOT National Transportation Integrated Search

2013-04-01

This report summarizes Test 3, a crippling load test on Budd Pioneer Car 244, conducted on June 28, 2011. Before the crippling load test, Transportation Technology Center, Inc., conducted two 800,000-pound (lb) quasi-static tests on Car 244 in accord...

Transit car demonstration test program on the roll dynamics unit. Volume 2. Demonstration of a transit car performance test on the roll dynamic unit. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arnold, G.; Nelson, S.; Cooperrider, N.K.

1982-02-11

This report, Volume II, contains the results, conclusions, and recommendations of the first performance test of a transit care on the RDU. This report is limited to performance tests. The objective is to identify advantages and disadvantages of performance testing on the rollers of the RDU as highlighted by the SOAC test. The report involved separate testing, done by TTC personnel, in such traditional performance areas of transit vehicle operation ass traction, acceleration/deceleration, energy consumption, and spin/slide performance. The results of the successful performance test of a transit car on a roller unit is presented and the advantages of thismore » method of testing is discussed. Acceleration, deceleration, spin/slide, and power consumption tests, although of limited scope in comparison to the track tests performed on the same transit car, did show the feasibility of roller testing. It is concluded that the RDU is most suited for developmental testing of transit car systems particularly for power consumption and for cars with non-standard wheel gage. Tests should be of such scope as to justify the cost of car setup on the RDU. The following two test are recommended: (1) a power consumption study for a standard/non-standard gage transit car which investigates methods of reducing power consumption and (2) a non-standard gage full performance test.« less

Two-car impact test of crash energy management passenger rail cars : analysis of occupant protection measurements

DOT National Transportation Integrated Search

2004-11-13

As a part of ongoing passenger rail equipment safety research, a full-scale impact test of two cars with energy absorbing end structures was carried out on February 26, 2004. In this test, two coupled cars impacted a rigid barrier at 29 mph. Similar ...

Postdoctoral Fellow | Center for Cancer Research

Cancer.gov

Dr. Mitchell Ho’s laboratory at the National Cancer Institute in Bethesda, Maryland, USA has an open postdoctoral position. We seek a highly motivated and creative individual to participate in a collaborative research project that involves the targeting of tumor-specific cell surface glypicans (e.g. GPC2, GPC3) using human T-cells engineered to express chimeric antigen receptors (CARs). The laboratory focuses on the understanding of the role of glypicans in cancer signaling, in particular Wnt signaling, and the development of CAR T cell therapies for treating liver cancer, neuroblastoma and other cancers. The successful candidate would be involved in isolation of human antibodies and camel or shark single domain antibodies using phage display, yeast display and mammalian cell display technologies, and production of CAR T cells, and preclinical testing of the CAR T cells using mouse tumor models. A recent paper related to the background of this project has been published in PNAS (https://www.ncbi.nlm.nih.gov/pubmed/28739923). Detailed information about Dr. Ho's research and publications can be accessed at: https://ccr.cancer.gov/mitchell-ho

Communication: Two-dimensional gas-phase coherent anti-Stokes Raman spectroscopy (2D-CARS): Simultaneous planar imaging and multiplex spectroscopy in a single laser shot

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bohlin, Alexis; Kliewer, Christopher J.

2013-01-01

Coherent anti-Stokes Raman spectroscopy (CARS) has been widely used as a powerful tool for chemical sensing, molecular dynamics measurements, and rovibrational spectroscopy since its development over 30 years ago, finding use in fields of study as diverse as combustion diagnostics, cell biology, plasma physics, and the standoff detection of explosives. The capability for acquiring resolved CARS spectra in multiple spatial dimensions within a single laser shot has been a long-standing goal for the study of dynamical processes, but has proven elusive because of both phase-matching and detection considerations. Here, by combining new phase matching and detection schemes with the highmore » efficiency of femtosecond excitation of Raman coherences, we introduce a technique for single-shot two-dimensional (2D) spatial measurements of gas phase CARS spectra. We demonstrate a spectrometer enabling both 2D plane imaging and spectroscopy simultaneously, and present the instantaneous measurement of 15, 000 spatially correlated rotational CARS spectra in N 2 and air over a 2D field of 40 mm 2.« less

Static aeroelastic analysis and tailoring of a single-element racing car wing

NASA Astrophysics Data System (ADS)

Sadd, Christopher James

This thesis presents the research from an Engineering Doctorate research programme in collaboration with Reynard Motorsport Ltd, a manufacturer of racing cars. Racing car wing design has traditionally considered structures to be rigid. However, structures are never perfectly rigid and the interaction between aerodynamic loading and structural flexibility has a direct impact on aerodynamic performance. This interaction is often referred to as static aeroelasticity and the focus of this research has been the development of a computational static aeroelastic analysis method to improve the design of a single-element racing car wing. A static aeroelastic analysis method has been developed by coupling a Reynolds-Averaged Navier-Stokes CFD analysis method with a Finite Element structural analysis method using an iterative scheme. Development of this method has included assessment of CFD and Finite Element analysis methods and development of data transfer and mesh deflection methods. Experimental testing was also completed to further assess the computational analyses. The computational and experimental results show a good correlation and these studies have also shown that a Navier-Stokes static aeroelastic analysis of an isolated wing can be performed at an acceptable computational cost. The static aeroelastic analysis tool was used to assess methods of tailoring the structural flexibility of the wing to increase its aerodynamic performance. These tailoring methods were then used to produce two final wing designs to increase downforce and reduce drag respectively. At the average operating dynamic pressure of the racing car, the computational analysis predicts that the downforce-increasing wing has a downforce of C[1]=-1.377 in comparison to C[1]=-1.265 for the original wing. The computational analysis predicts that the drag-reducing wing has a drag of C[d]=0.115 in comparison to C[d]=0.143 for the original wing.

Development and Evaluation of an Optimal Human Single-Chain Variable Fragment-Derived BCMA-Targeted CAR T Cell Vector.

PubMed

Smith, Eric L; Staehr, Mette; Masakayan, Reed; Tatake, Ishan J; Purdon, Terence J; Wang, Xiuyan; Wang, Pei; Liu, Hong; Xu, Yiyang; Garrett-Thomson, Sarah C; Almo, Steven C; Riviere, Isabelle; Liu, Cheng; Brentjens, Renier J

2018-06-06

B cell maturation antigen (BCMA) has recently been identified as an important multiple myeloma (MM)-specific target for chimeric antigen receptor (CAR) T cell therapy. In CAR T cell therapy targeting CD19 for lymphoma, host immune anti-murine CAR responses limited the efficacy of repeat dosing and possibly long-term persistence. This clinically relevant concern can be addressed by generating a CAR incorporating a human single-chain variable fragment (scFv). We screened a human B cell-derived scFv phage display library and identified a panel of BCMA-specific clones from which human CARs were engineered. Despite a narrow range of affinity for BCMA, dramatic differences in CAR T cell expansion were observed between unique scFvs in a repeat antigen stimulation assay. These results were confirmed by screening in a MM xenograft model, where only the top preforming CARs from the repeat antigen stimulation assay eradicated disease and prolonged survival. The results of this screening identified a highly effective CAR T cell therapy with properties, including rapid in vivo expansion (>10,000-fold, day 6), eradication of large tumor burden, and durable protection to tumor re-challenge. We generated a bicistronic construct including a second-generation CAR and a truncated-epithelial growth factor receptor marker. CAR T cell vectors stemming from this work are under clinical investigation. Copyright © 2018 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.

Crash Testing in the Lab: Putting a New Stop to the CO2 Car!

ERIC Educational Resources Information Center

Decker, Rob

2005-01-01

Every year in the United States, the federal government, insurance companies and automobile manufacturers wreck hundreds of cars for safety-testing purposes. All this crashing comes in an effort to rate vehicular safety for the public. Inspired by numerous movies with car chases, dramatic wrecks and television commercials showing car safety tests,…

Car seat safety: literature review.

PubMed

Lincoln, Michelle

2005-01-01

After staggering numbers of infants were killed in automotive crashes in the 1970s, the American Academy of Pediatrics (AAP) recommended in 1974 universal use of car seats for all infants. However, positional problems were reported when car seats are used with premature infants less than 37 weeks gestational age as a result of head slouching and its sequelae. In 1990, the AAP responded with another policy statement introducing car seat testing. It recommended that any infant at or under 37 weeks gestational age be observed in a car seat prior to discharge from the hospital. The AAP did not give specific guidelines on type of car seat, length of testing, equipment, or personnel proficiency, however. Few nurseries have standard policies to evaluate car seats, to teach parents about car seats, or to position newborns in them, and not all hospitals actually conduct car seat challenges or have common standards for testing that is performed.

Side Impact Regulatory Trends, Crash Environment and Injury Risk in the USA.

PubMed

Prasad, Priya; Dalmotas, Dainius; Chouinard, Aline

2015-11-01

Light duty vehicles in the US are designed to meet and exceed regulatory standards, self-imposed industry agreements and safety rating tests conducted by NHTSA and IIHS. The evolution of side impact regulation in the US from 1973 to 2015 is discussed in the paper along with two key industry agreements in 2003 affecting design of restraint systems and structures for side impact protection. A combination of all the above influences shows that vehicles in the US are being designed to more demanding and comprehensive requirements than in any other region of the world. The crash environment in the US related to side impacts was defined based on data in the nationally representative crash database NASS. Crash environment factors, including the distribution of cars, light trucks and vans (LTV's), and medium-to-heavy vehicles (MHV's) in the fleet, and the frequency of their interactions with one another in side impacts, were considered. Other factors like, crash severity in terms of closing velocity between two vehicles involved in crash, gender and age of involved drivers in two-vehicle and single vehicle crashes, were also examined. Injury risks in side impacts to drivers and passengers were determined in various circumstances such as near-side, far-side, and single vehicle crashes as a function of crash severity, in terms of estimated closing speed or lateral delta-V. Also injury risks in different pairs of striking and struck cars and LTV's, were estimated. A logistic regression model for studying injury risks in two vehicle crashes was developed. The risk factors included in the model include case and striking vehicles, consisting of cars, SUV's, vans, and pickup trucks, delta-V, damage extent, occupant proximity to the impact side, age and gender of the occupant, and belt use. Results show that car occupants make up the vast majority of serious-to-fatally injured occupants. Injury rates of car occupants in two-vehicle collision are highest when the car is struck by a pickup and lowest when struck by a car. This was the case across all lateral delta-V ranges. Additionally, near-side injury rates are substantially higher than those in far-side impacts.

Split-probe hybrid femtosecond/picosecond rotational CARS for time-domain measurement of S-branch Raman linewidths within a single laser shot.

PubMed

Patterson, Brian D; Gao, Yi; Seeger, Thomas; Kliewer, Christopher J

2013-11-15

We introduce a multiplex technique for the single-laser-shot determination of S-branch Raman linewidths with high accuracy and precision by implementing hybrid femtosecond (fs)/picosecond (ps) rotational coherent anti-Stokes Raman spectroscopy (CARS) with multiple spatially and temporally separated probe beams derived from a single laser pulse. The probe beams scatter from the rotational coherence driven by the fs pump and Stokes pulses at four different probe pulse delay times spanning 360 ps, thereby mapping collisional coherence dephasing in time for the populated rotational levels. The probe beams scatter at different folded BOXCARS angles, yielding spatially separated CARS signals which are collected simultaneously on the charge coupled device camera. The technique yields a single-shot standard deviation (1σ) of less than 3.5% in the determination of Raman linewidths and the average linewidth values obtained for N(2) are within 1% of those previously reported. The presented technique opens the possibility for correcting CARS spectra for time-varying collisional environments in operando.

Full scale tank car coupler impact tests

DOT National Transportation Integrated Search

2003-11-15

Full scale tests were performed to investigate various : aspects of tank car behavior during coupler impacts. A tank car : was equipped with 37 accelerometers and an instrumented : coupler. Two series of full scale coupler impact tests, : comprising ...

In-vehicle VOCs composition of unconditioned, newly produced cars.

PubMed

Brodzik, Krzysztof; Faber, Joanna; Łomankiewicz, Damian; Gołda-Kopek, Anna

2014-05-01

The in-vehicle volatile organic compounds (VOCs) concentrations gains the attention of both car producers and users. In the present study, an attempt was made to determine if analysis of air samples collected from an unconditioned car cabin can be used as a quality control measure. The VOCs composition of in-vehicle air was analyzed by means of active sampling on Carbograph 1TD and Tenax TA sorbents, followed by thermal desorption and simultaneous analysis on flame ionization and mass detector (TD-GC/FID-MS). Nine newly produced cars of the same brand and model were chosen for this study. Within these, four of the vehicles were equipped with identical interior materials and five others differed in terms of upholstery and the presence of a sunroof; one car was convertible. The sampling event took place outside of the car assembly plant and the cars tested left the assembly line no later than 24 hr before the sampling took place. More than 250 compounds were present in the samples collected; the identification of more than 160 was confirmed by comparative mass spectra analysis and 80 were confirmed by both comparison with single/multiple compounds standards and mass spectra analysis. In general, aliphatic hydrocarbons represented more than 60% of the total VOCs (TVOC) determined. Depending on the vehicle, the concentration of aromatic hydrocarbons varied from 12% to 27% of total VOCs. The very short period between car production and sampling of the in-vehicle air permits the assumption that the entire TVOC originates from off-gassing of interior materials. The results of this study expand the knowledge of in-vehicle pollution by presenting information about car cabin air quality immediately after car production. Copyright © 2014 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.

Crashworthiness requirements for commuter rail passenger seats

DOT National Transportation Integrated Search

2005-11-05

Occupant experiments using instrumented crash test dummies seated in commuter rail seats have been conducted on board full-scale impact tests of rail cars. The tests have been conducted using both conventional cars and cars modified to incorporate cr...

Car Builder: Design, Construct and Test Your Own Cars. School Version with Lesson Plans. [CD-ROM].

ERIC Educational Resources Information Center

Highsmith, Joni Bitman

Car Builder is a scientific CD-ROM-based simulation program that lets students design, construct, modify, test, and compare their own cars. Students can design sedans, four-wheel-drive vehicles, vans, sport cars, and hot rods. They may select for aerodynamics, power, and racing ability, or economic and fuel efficiency. It is a program that teaches…

Effects of coupler height mismatch on the structural integrity of railroad tank car stub sills.

DOT National Transportation Integrated Search

2001-12-01

This project evaluated the safety implications of coupler height mismatches on the integrity of railroad tank car stub sills, through a series of static and impact tests. The test car was a loaded tank car instrumented with strain gages at critical l...

49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

Code of Federal Regulations, 2010 CFR

2010-10-01

... specification tank cars. 180.509 Section 180.509 Transportation Other Regulations Relating to Transportation... REGULATIONS CONTINUING QUALIFICATION AND MAINTENANCE OF PACKAGINGS Qualification and Maintenance of Tank Cars § 180.509 Requirements for inspection and test of specification tank cars. (a) General. (1) Each tank...

An improved car-following model considering headway changes with memory

NASA Astrophysics Data System (ADS)

Yu, Shaowei; Shi, Zhongke

2015-03-01

To describe car-following behaviors in complex situations better, increase roadway traffic mobility and minimize cars' fuel consumptions, the linkage between headway changes with memory and car-following behaviors was explored with the field car-following data by using the gray correlation analysis method, and then an improved car-following model considering headway changes with memory on a single lane was proposed based on the full velocity difference model. Some numerical simulations were carried out by employing the improved car-following model to explore how headway changes with memory affected each car's velocity, acceleration, headway and fuel consumptions. The research results show that headway changes with memory have significant effects on car-following behaviors and fuel consumptions and that considering headway changes with memory in designing the adaptive cruise control strategy can improve the traffic flow stability and minimize cars' fuel consumptions.

Accident or Suicide? Single-Vehicle Car Accidents and the Intent Hypothesis.

ERIC Educational Resources Information Center

Peck, Dennis L.; Warner, Kenneth

1995-01-01

Brief case studies of single-vehicle car crashes are used to explore the relationship between this method of death and the intent to commit suicide. The author attempts to determine what criteria can be used to determine whether suicide was the intent, and how death by automobile crash can be determined a suicide. (JPS)

Cytogenetic biomonitoring of peripheral blood and oral mucosa cells from car painters.

PubMed

Pereira da Silva, Victor Hugo; Gomes de Moura, Carolina Foot; Spadari-Bratfisch, Regina Célia; Araki Ribeiro, Daniel

2012-09-01

The aim of the present study was to comparatively evaluate genomic damage and cellular death in exfoliated oral mucosa cells and peripheral blood from car painters. A total of 24 car painters and 19 healthy controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mucosa (left and right side) mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the specific nuclear phenotypes. A total of 5 μL from peripheral blood was collected for the single cell gel (comet) assay. The results pointed out statistically significant differences (p < 0.05) of micronucleated oral mucosa cells from car painters. In addition, DNA damage was detected in peripheral blood cells by single cell gel (comet) assay. Nevertheless, exposure to car paints did not cause increases other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis and karyolysis in buccal mucosa cells. In summary, the results of the present study suggest that car painters comprise a high risk group since paints can induce genotoxic and mutagenic effects in peripheral blood and oral mucosa cells, respectively.

A study of emissions from passenger cars in six cities. Volume B. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1979-01-01

This is the second of two volumes presenting results from a series of exhaust emission and fuel economy tests performed on a representative sample of vehicles in six U.S. cities. Data presented in the following sections are generated in appendix form as part of a contract with the EPA to perform work for the FY 77 Passenger Car Emission Factor Program. Volume B includes the balance of individual vehicle data derived from the Two Speed Idle and Federal Three Mode tests. It also includes the results of Vehicle Driveability Evaluations, Maladjustment and Disablement Inspections, tire inspections and a listing ofmore » comparative mileage data. Information presented in Volume B should not be interpreted without the benefit of additional descriptive data presented in Volume A as both volumes comprise the results of a single work effort and are not intended to be considered separately.« less

Roof strength and injury risk in rollover crashes of passenger cars.

PubMed

Brumbelow, Matthew L; Teoh, Eric R

2009-12-01

A 2009 study by the Insurance Institute for Highway Safety found that midsize SUVs with stronger roofs, as measured in quasi-static tests, had lower risk of ejection and lower risk of injury for nonejected drivers. The objective of the present study was to determine whether a similar association exists for other vehicle groups. Twelve small passenger cars were evaluated according to Federal Motor Vehicle Safety Standard 216 test conditions. Crash databases in 14 states provided more than 20,000 single-vehicle rollover crashes involving these vehicles. Logistic regression analyses were used to evaluate the effect of roof strength on the rate of driver injury while assessing and controlling for the effects of driver age, vehicle stability, state, and other factors where necessary. Small cars with stronger roofs had lower overall rates of serious injury, lower rates of ejection, and lower rates of injury for nonejected drivers. Although the effect on ejection was somewhat smaller for cars than for SUVs, the overall pattern of injury results was consistent. For roof strength-to-weight ratio measured within 5 in. (SWR(5)), a one-unit increase (e.g., from 2.0 to 3.0) was associated with a 22 percent reduction in risk of incapacitating or fatal driver injury in single-vehicle rollovers. This compares with a 24 percent reduction estimated for a similar change in roof strength among midsize SUVs. The association between vehicle roof strength and occupant injury risk in rollover crashes appears robust across different vehicle groups and across roof SWR(5) values, varying from just more than 1.5 to just less than 4.0. If roofs were to increase in strength by one SWR(5), a 20-25 percent reduction in risk of serious injury in rollovers would be expected. Still, even if all vehicle roofs were as strong as the strongest roof measured, many rollover injuries still would occur, indicating the need for additional research and countermeasures.

Simulation study of traffic car accidents at a single lane roundabout

NASA Astrophysics Data System (ADS)

Echab, H.; Lakouari, N.; Ez-Zahraouy, H.; Benyoussef, A.

2016-07-01

In this paper, using the Nagel-Schreckenberg model, we numerically investigate the probability Pac of entering/circulating car accidents to occur at single-lane roundabout under the expanded open boundary. The roundabout consists of N on-ramps (respectively, off-ramps). The boundary is controlled by the injecting rates α1,α2 and the extracting rate β. The simulation results show that, depending on the injecting rates, the car accidents are more likely to happen when the capacity of the rotary is set to its maximum. Moreover, we found that the large values of rotary size L and the probability of preferential Pexit are reliable to improve safety and reduce accidents. However, the usage of indicator, the increase of β and/or N provokes an increase of car accident probability.

Behavior of amphibians on the road in response to car traffic

USGS Publications Warehouse

Mazerolle, M.J.; Huot, M.; Gravel, M.

2005-01-01

Nocturnal car traffic often results in amphibian casualties, especially during rainy nights. The behavior of amphibians presumably influences their vulnerability to mortality on the road, but this hypothesis remains untested. We investigated the behavioral response of individuals of six species of amphibians on roads when confronted by an approaching vehicle. We first conducted a field study consisting of 50 night-driving surveys over 4 yr during which we recorded the behavior (i.e., moving or immobile) of frogs, toads, tree frogs, and salamanders encountered on a 20-km stretch of road. In an effort to tease apart the effects of headlights and the sound of motors on amphibian behavior, we carried out a field experiment on a test road where we exposed individuals to different car-associated stimuli. Here, we tested the hypothesis that simultaneous exposure to headlights and the sound of a car motor would elicit a stronger response than exposure to a single stimulus or a control. Based on the observations of the 2767 individuals in the field survey, immobility was the most common response to the approach of a car (mean probability of 0.82 of remaining immobile); the response differed across species but depended on the season of the survey (May-June vs. July-September). Similarly, the 91 individuals included in the field experiment were more likely to move during the control treatment than during any of the car-associated treatments. The combined stimuli elicited the strongest response, followed by the headlights-only and the motor-only treatments. Spring peepers (Pseudacris crucifer) tended to move more often than the other species we tested in the field experiment, which suggests they spend less time on the road and are less vulnerable to traffic mortality than other species. Both the field survey and field experiment consistently indicated that amphibians tend to remain immobile at the approach of a vehicle. This behavior highlights the vulnerability of amphibians to road traffic and should be considered in measures to mitigate road impacts. ?? 2005 by The Herpetologists' League, Inc.

Identification of chimeric antigen receptors that mediate constitutive or inducible proliferation of T cells.

PubMed

Frigault, Matthew J; Lee, Jihyun; Basil, Maria Ciocca; Carpenito, Carmine; Motohashi, Shinichiro; Scholler, John; Kawalekar, Omkar U; Guedan, Sonia; McGettigan, Shannon E; Posey, Avery D; Ang, Sonny; Cooper, Laurence J N; Platt, Jesse M; Johnson, F Brad; Paulos, Chrystal M; Zhao, Yangbing; Kalos, Michael; Milone, Michael C; June, Carl H

2015-04-01

This study compared second-generation chimeric antigen receptors (CAR) encoding signaling domains composed of CD28, ICOS, and 4-1BB (TNFRSF9). Here, we report that certain CARs endow T cells with the ability to undergo long-term autonomous proliferation. Transduction of primary human T cells with lentiviral vectors encoding some of the CARs resulted in sustained proliferation for up to 3 months following a single stimulation through the T-cell receptor (TCR). Sustained numeric expansion was independent of cognate antigen and did not require the addition of exogenous cytokines or feeder cells after a single stimulation of the TCR and CD28. Results from gene array and functional assays linked sustained cytokine secretion and expression of T-bet (TBX21), EOMES, and GATA-3 to the effect. Sustained expression of the endogenous IL2 locus has not been reported in primary T cells. Sustained proliferation was dependent on CAR structure and high expression, the latter of which was necessary but not sufficient. The mechanism involves constitutive signaling through NF-κB, AKT, ERK, and NFAT. The propagated CAR T cells retained a diverse TCR repertoire, and cellular transformation was not observed. The CARs with a constitutive growth phenotype displayed inferior antitumor effects and engraftment in vivo. Therefore, the design of CARs that have a nonconstitutive growth phenotype may be a strategy to improve efficacy and engraftment of CAR T cells. The identification of CARs that confer constitutive or nonconstitutive growth patterns may explain observations that CAR T cells have differential survival patterns in clinical trials. ©2015 American Association for Cancer Research.

An experimental investigation of tobacco smoke pollution in cars.

PubMed

Sendzik, Taryn; Fong, Geoffrey T; Travers, Mark J; Hyland, Andrew

2009-06-01

Tobacco smoke pollution (TSP) has been identified as a serious public health threat. Although the number of jurisdictions that prohibit smoking in public places has increased rapidly, just a few successful attempts have been made to pass similar laws prohibiting smoking in cars, where the cabin space may contribute to concentrated exposure. In particular, TSP constitutes a potentially serious health hazard to children because of prolonged exposure and their small size. The present study investigated the levels of TSP in 18 cars via the measurement of fine respirable particles (<2.5 microns in diameter or PM(2.5)) under a variety of in vivo conditions. Car owners smoked a single cigarette in their cars in each of five controlled air-sampling conditions. Each condition varied on movement of the car, presence of air conditioning, open windows, and combinations of these airflow influences. Smoking just a single cigarette in a car generated extremely high average levels of PM(2.5): more than 3,800 microg/m3 in the condition with the least airflow (motionless car, windows closed). In moderate ventilation conditions (air conditioning or having the smoking driver hold the cigarette next to a half-open window), the average levels of PM(2.5) were reduced but still at significantly high levels (air conditioning = 844 microg/m3; holding cigarette next to a half-open window = 223 microg/m3). This study demonstrates that TSP in cars reaches unhealthy levels, even under realistic ventilation conditions, lending support to efforts occurring across a growing number of jurisdictions to educate people and prohibit smoking in cars in the presence of children.

Disruption of TET2 promotes the therapeutic efficacy of CD19-targeted T cells.

PubMed

Fraietta, Joseph A; Nobles, Christopher L; Sammons, Morgan A; Lundh, Stefan; Carty, Shannon A; Reich, Tyler J; Cogdill, Alexandria P; Morrissette, Jennifer J D; DeNizio, Jamie E; Reddy, Shantan; Hwang, Young; Gohil, Mercy; Kulikovskaya, Irina; Nazimuddin, Farzana; Gupta, Minnal; Chen, Fang; Everett, John K; Alexander, Katherine A; Lin-Shiao, Enrique; Gee, Marvin H; Liu, Xiaojun; Young, Regina M; Ambrose, David; Wang, Yan; Xu, Jun; Jordan, Martha S; Marcucci, Katherine T; Levine, Bruce L; Garcia, K Christopher; Zhao, Yangbing; Kalos, Michael; Porter, David L; Kohli, Rahul M; Lacey, Simon F; Berger, Shelley L; Bushman, Frederic D; June, Carl H; Melenhorst, J Joseph

2018-06-01

Cancer immunotherapy based on genetically redirecting T cells has been used successfully to treat B cell malignancies 1-3 . In this strategy, the T cell genome is modified by integration of viral vectors or transposons encoding chimaeric antigen receptors (CARs) that direct tumour cell killing. However, this approach is often limited by the extent of expansion and persistence of CAR T cells 4,5 . Here we report mechanistic insights from studies of a patient with chronic lymphocytic leukaemia treated with CAR T cells targeting the CD19 protein. Following infusion of CAR T cells, anti-tumour activity was evident in the peripheral blood, lymph nodes and bone marrow; this activity was accompanied by complete remission. Unexpectedly, at the peak of the response, 94% of CAR T cells originated from a single clone in which lentiviral vector-mediated insertion of the CAR transgene disrupted the methylcytosine dioxygenase TET2 gene. Further analysis revealed a hypomorphic mutation in this patient's second TET2 allele. TET2-disrupted CAR T cells exhibited an epigenetic profile consistent with altered T cell differentiation and, at the peak of expansion, displayed a central memory phenotype. Experimental knockdown of TET2 recapitulated the potency-enhancing effect of TET2 dysfunction in this patient's CAR T cells. These findings suggest that the progeny of a single CAR T cell induced leukaemia remission and that TET2 modification may be useful for improving immunotherapies.

Modification of Expanded NK Cells with Chimeric Antigen Receptor mRNA for Adoptive Cellular Therapy.

PubMed

Chu, Yaya; Flower, Allyson; Cairo, Mitchell S

2016-01-01

NK cells are bone marrow-derived cytotoxic lymphocytes that play a major role in the rejection of tumors and cells infected by viruses. The regulation of NK activation vs inhibition is regulated by the expression of a variety of NK receptors (NKRs) and specific NKRs' ligands expressed on their targets. However, factors limiting NK therapy include small numbers of active NK cells in unexpanded peripheral blood and lack of specific tumor targeting. Chimeric antigen receptors (CAR) usually include a single-chain Fv variable fragment from a monoclonal antibody, a transmembrane hinge region, and a signaling domain such as CD28, CD3-zeta, 4-1BB (CD137), or 2B4 (CD244) endodimers. Redirecting NK cells with a CAR will circumvent the limitations of the lack of NK targeting specificity. This chapter focuses on the methods to expand human NK cells from peripheral blood by co-culturing with feeder cells and to modify the expanded NK cells efficiently with the in vitro transcribed CAR mRNA by electroporation and to test the functionality of the CAR-modified expanded NK cells for use in adoptive cellular immunotherapy.

Improving CAR Navigation with a Vision-Based System

NASA Astrophysics Data System (ADS)

Kim, H.; Choi, K.; Lee, I.

2015-08-01

The real-time acquisition of the accurate positions is very important for the proper operations of driver assistance systems or autonomous vehicles. Since the current systems mostly depend on a GPS and map-matching technique, they show poor and unreliable performance in blockage and weak areas of GPS signals. In this study, we propose a vision oriented car navigation method based on sensor fusion with a GPS and in-vehicle sensors. We employed a single photo resection process to derive the position and attitude of the camera and thus those of the car. This image georeferencing results are combined with other sensory data under the sensor fusion framework for more accurate estimation of the positions using an extended Kalman filter. The proposed system estimated the positions with an accuracy of 15 m although GPS signals are not available at all during the entire test drive of 15 minutes. The proposed vision based system can be effectively utilized for the low-cost but high-accurate and reliable navigation systems required for intelligent or autonomous vehicles.

Improving Car Navigation with a Vision-Based System

NASA Astrophysics Data System (ADS)

Kim, H.; Choi, K.; Lee, I.

2015-08-01

The real-time acquisition of the accurate positions is very important for the proper operations of driver assistance systems or autonomous vehicles. Since the current systems mostly depend on a GPS and map-matching technique, they show poor and unreliable performance in blockage and weak areas of GPS signals. In this study, we propose a vision oriented car navigation method based on sensor fusion with a GPS and in-vehicle sensors. We employed a single photo resection process to derive the position and attitude of the camera and thus those of the car. This image georeferencing results are combined with other sensory data under the sensor fusion framework for more accurate estimation of the positions using an extended Kalman filter. The proposed system estimated the positions with an accuracy of 15 m although GPS signals are not available at all during the entire test drive of 15 minutes. The proposed vision based system can be effectively utilized for the low-cost but high-accurate and reliable navigation systems required for intelligent or autonomous vehicles.

75 FR 30105 - Petition for Waiver of Compliance

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-28

... requirements for testing locomotive horns. LIRR operates 836 M-7 MU passenger cars of which 84 have had their horns tested; and 170 M-3 MU passenger cars of which 60 have had their horns tested. In addition, LIRR operates 80 diesel electric locomotives of which 7 horns have been tested; and 23 control car locomotives...

Passenger cab car grade crossing impact test report : rail passenger equipment impact tests

DOT National Transportation Integrated Search

2007-10-01

Two full-scale oblique grade crossing impact tests were conducted in June 2002 to compare the crashworthiness performance of alternative corner post designs on rail passenger cab cars. On June 4, 2002, a cab car fitted with an end frame built to pre-...

Transit Car Performance Comparison, State-of-the-Art Car vs. PATCO Transit Car, NYCTA R-46, MBTA Silverbirds

DOT National Transportation Integrated Search

1978-02-01

The first phase of this contract authorized the design, development, and demonstration of two State-Of-The-Art Cars (SOAC). This document reports on the gathering of comparative test data on existing in-service transit cars. The three transit cars se...

Small passenger car transmission test: Dodge Omni A-404 transmission

NASA Technical Reports Server (NTRS)

Bujold, M. P.

1980-01-01

The small passenger car transmission test was initiated to supply electric vehicle manufacturers with technical information regarding the performance of commercially available transmissions. This transmission was tested in accordance with a passenger car automatic transmission test code (SAE J65lb) which required drive performance, coast performance, and no load test conditions. Under these test conditions, the transmission attained maximum efficiencies in the mid eighty percent range for both drive performance test and coast performance tests.

An evaluation of automated chest radiography reading software for tuberculosis screening among public- and private-sector patients.

PubMed

Rahman, Md Toufiq; Codlin, Andrew J; Rahman, Md Mahfuzur; Nahar, Ayenun; Reja, Mehdi; Islam, Tariqul; Qin, Zhi Zhen; Khan, Md Abdus Shakur; Banu, Sayera; Creswell, Jacob

2017-05-01

Computer-aided reading (CAR) of medical images is becoming increasingly common, but few studies exist for CAR in tuberculosis (TB). We designed a prospective study evaluating CAR for chest radiography (CXR) as a triage tool before Xpert MTB/RIF (Xpert).Consecutively enrolled adults in Dhaka, Bangladesh, with TB symptoms received CXR and Xpert. Each image was scored by CAR and graded by a radiologist. We compared CAR with the radiologist for sensitivity and specificity, area under the receiver operating characteristic curve (AUC), and calculated the potential Xpert tests saved.A total of 18 036 individuals were enrolled. TB prevalence by Xpert was 15%. The radiologist graded 49% of CXRs as abnormal, resulting in 91% sensitivity and 58% specificity. At a similar sensitivity, CAR had a lower specificity (41%), saving fewer (36%) Xpert tests. The AUC for CAR was 0.74 (95% CI 0.73-0.75). CAR performance declined with increasing age. The radiologist grading was superior across all sub-analyses.Using CAR can save Xpert tests, but the radiologist's specificity was superior. Differentiated CAR thresholds may be required for different populations. Access to, and costs of, human readers must be considered when deciding to use CAR software. More studies are needed to evaluate CAR using different screening approaches. Copyright ©ERS 2017.

An evaluation of automated chest radiography reading software for tuberculosis screening among public- and private-sector patients

PubMed Central

Rahman, Md Toufiq; Codlin, Andrew J.; Rahman, Md Mahfuzur; Nahar, Ayenun; Reja, Mehdi; Islam, Tariqul; Qin, Zhi Zhen; Khan, Md Abdus Shakur; Banu, Sayera

2017-01-01

Computer-aided reading (CAR) of medical images is becoming increasingly common, but few studies exist for CAR in tuberculosis (TB). We designed a prospective study evaluating CAR for chest radiography (CXR) as a triage tool before Xpert MTB/RIF (Xpert). Consecutively enrolled adults in Dhaka, Bangladesh, with TB symptoms received CXR and Xpert. Each image was scored by CAR and graded by a radiologist. We compared CAR with the radiologist for sensitivity and specificity, area under the receiver operating characteristic curve (AUC), and calculated the potential Xpert tests saved. A total of 18 036 individuals were enrolled. TB prevalence by Xpert was 15%. The radiologist graded 49% of CXRs as abnormal, resulting in 91% sensitivity and 58% specificity. At a similar sensitivity, CAR had a lower specificity (41%), saving fewer (36%) Xpert tests. The AUC for CAR was 0.74 (95% CI 0.73–0.75). CAR performance declined with increasing age. The radiologist grading was superior across all sub-analyses. Using CAR can save Xpert tests, but the radiologist's specificity was superior. Differentiated CAR thresholds may be required for different populations. Access to, and costs of, human readers must be considered when deciding to use CAR software. More studies are needed to evaluate CAR using different screening approaches. PMID:28529202

49 CFR 232.205 - Class I brake test-initial terminal inspection.

Code of Federal Regulations, 2010 CFR

2010-10-01

... § 232.205 Class I brake test-initial terminal inspection. (a) Each train and each car in the train shall... tested solid block of cars to the train; (iii) Changing motive power; (iv) Removing or changing the... conduct a brake test pursuant to § 232.209 on those cars added to the train. (B) [Reserved] (b) Except as...

Full-scale locomotive dynamic crash testing and correlations : C-39 type locomotive colliding with a loaded hopper car (test 7).

DOT National Transportation Integrated Search

2011-09-01

This report presents the results of a locomotive and three loaded hopper car consist traveling at 29 miles per hour colliding with a stationary consist of 35 loaded hopper cars. The details of test instrumentation, LS-DYNA finite element simulation, ...

An experimental investigation of tobacco smoke pollution in cars

PubMed Central

Sendzik, Taryn; Travers, Mark J.; Hyland, Andrew

2009-01-01

Introduction Tobacco smoke pollution (TSP) has been identified as a serious public health threat. Although the number of jurisdictions that prohibit smoking in public places has increased rapidly, just a few successful attempts have been made to pass similar laws prohibiting smoking in cars, where the cabin space may contribute to concentrated exposure. In particular, TSP constitutes a potentially serious health hazard to children because of prolonged exposure and their small size. Methods The present study investigated the levels of TSP in 18 cars via the measurement of fine respirable particles (<2.5 microns in diameter or PM2.5) under a variety of in vivo conditions. Car owners smoked a single cigarette in their cars in each of five controlled air-sampling conditions. Each condition varied on movement of the car, presence of air conditioning, open windows, and combinations of these airflow influences. Results Smoking just a single cigarette in a car generated extremely high average levels of PM2.5: more than 3,800 μg/m3 in the condition with the least airflow (motionless car, windows closed). In moderate ventilation conditions (air conditioning or having the smoking driver hold the cigarette next to a half-open window), the average levels of PM2.5 were reduced but still at significantly high levels (air conditioning = 844 μg/m3; holding cigarette next to a half-open window = 223 μg/m3). Discussion This study demonstrates that TSP in cars reaches unhealthy levels, even under realistic ventilation conditions, lending support to efforts occurring across a growing number of jurisdictions to educate people and prohibit smoking in cars in the presence of children. PMID:19351785

Results of baseline tests of the EVA metro sedan, citi-car, jet industries electra-van, CDA town car, and OTIS P-500 van

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stenger, F.J.; Bozek, J.M.; Soltis, R.F.

1976-10-01

Five electric vehicles were tested at vehicle test tracks using the SAE. The tests provide range data at steady speeds and for several driving cycles. Most tests were conducted with lead-acid traction batteries. The Otis Van and the Copper Electric Town Car were also tested with lead-acid and nickel-zinc batteries. The tests showed a range increase of from 82 to 101 percent depending on vehicle, speed, and test cycle.

Results of baseline tests of the EVA Metro sedan, Citi-car, Jet Industries Electra-van, CDA town car, and Otis P-500 van

NASA Technical Reports Server (NTRS)

Stenger, F. J.; Bozek, J. M.; Soltis, R. F.

1976-01-01

Five electric vehicles were tested at vehicle test tracks using the SAE. The tests provide range data at steady speeds and for several driving cycles. Most tests were conducted with lead-acid traction batteries. The Otis Van and the Copper Electric Town Car were also tested with lead-acid and nickel-zinc batteries. The tests showed a range increase of from 82 to 101 percent depending on vehicle, speed, and test cycle.

Repeated-dose effects of mequitazine, cetirizine and dexchlorpheniramine on driving and psychomotor performance.

PubMed

Theunissen, Eef L; Vermeeren, Annemiek; Ramaekers, Johannes G

2006-01-01

Previous studies have demonstrated that the antihistamines mequitazine, cetirizine and dexchlorpheniramine produce mild sedation after single doses. It is unknown, however, whether acute sedation persists after repeated dosing. Therefore, this study assessed the effects of repeated dosing of these antihistamines on driving and psychomotor performance. Sixteen healthy volunteers were treated with mequitazine 10 mg q.a.m., cetirizine 10 mg q.a.m., dexchlorpheniramine Repetab 6 mg b.i.d. and placebo for four separate 8-day periods. Drug effects were assessed on days 1 and 8 using on-the-road driving tests (highway driving and car following), psychomotor tests (tracking and divided attention) and subjective questionnaires. Dexchlorpheniramine and mequitazine significantly impaired driving performance on the highway driving test on the first day; dexchlorpheniramine increased Standard Deviation of Lateral Position by 2 cm [95% confidence interval (CI) 0.5, 3.8] and mequitazine by 2.5 cm (CI 1.0, 4.3). These effects on driving performance disappeared after 8 days of treatment. No effect of treatment was found on car following, tracking and divided attention. Although subjective ratings confirmed that subjects knew their driving had been impaired in the mequitazine and dexchlorpheniramine condition after completion of the highway driving test on day 1, they did not expect their driving to be affected before the start of the test. Cetirizine did not impair performance on any of the tests. Single doses of mequitazine 10 mg and dexchlorpheniramine Repetab 6 mg cause mild driving impairment. However, when taken over several days, the impairing effect wears off, possibly as a result of tolerance.

Identification of chimeric antigen receptors that mediate constitutive or inducible proliferation of T cells

PubMed Central

Frigault, Matthew J; Lee, Jihyun; Basil, Maria Ciocca; Carpenito, Carmine; Motohashi, Shinichiro; Scholler, John; Kawalekar, Omkar U.; Guedan, Sonia; McGettigan, Shannon E.; Posey, Avery D.; Ang, Sonny; Cooper, Laurence J. N.; Platt, Jesse M.; Johnson, F. Brad; Paulos, Chrystal M; Zhao, Yangbing; Kalos, Michael; Milone, Michael C.; June, Carl H.

2015-01-01

This study compared second generation chimeric antigen receptors encoding signaling domains composed of CD28, ICOS and 4-1BB. Here we report that certain CARs endow T cells with the ability to undergo long-term autonomous proliferation. Transduction of primary human T-cell with lentiviral vectors encoding some of the CARs resulted in sustained proliferation for up to three months following a single stimulation through the TCR. Sustained numeric expansion was independent of cognate antigen and did not require the addition of exogenous cytokines or feeder cells after a single stimulation of the TCR and CD28. Results from gene array and functional assays linked sustained cytokine secretion and expression of T-bet, EOMES and GATA-3 to the effect. Sustained expression of the endogenous IL2 locus has not been reported in primary T cells. Sustained proliferation was dependent on CAR structure and high expression, the latter of which was necessary but not sufficient. The mechanism involves constitutive signaling through NF-kB, Akt, Erk and NFAT. The propagated CAR T cells retained a diverse TCR repertoire and cellular transformation was not observed. The CARs with a constitutive growth phenotype displayed inferior antitumor effects and engraftment in vivo. Therefore the design of CARs that have a non-constitutive growth phenotype may be a strategy to improve efficacy and engraftment of CAR T cells. The identification of CARs that confer constitutive or non-constitutive growth patterns may explain observations that CAR T cells have differential survival patterns in clinical trials. PMID:25600436

Clinical multiphoton and CARS microscopy

NASA Astrophysics Data System (ADS)

Breunig, H. G.; Weinigel, M.; Darvin, M. E.; Lademann, J.; König, K.

2012-03-01

We report on clinical CARS imaging of human skin in vivo with the certified hybrid multiphoton tomograph CARSDermaInspect. The CARS-DermaInspect provides simultaneous imaging of non-fluorescent intradermal lipid and water as well as imaging of two-photon excited fluorescence from intrinsic molecules. Two different excitation schemes for CARS imaging have been realized: In the first setup, a combination of fs oscillator and optical parametric oscillator provided fs-CARS pump and Stokes pulses, respectively. In the second setup a fs oscillator was combined with a photonic crystal fiber which provided a broadband spectrum. A spectral range out of the broadband-spectrum was selected and used for CARS excitation in combination with the residual fs-oscillator output. In both setups, in addition to CARS, single-beam excitation was used for imaging of two-photon excited fluorescence and second harmonic generation signals. Both CARS-excitation systems were successfully used for imaging of lipids inside the skin in vivo.

Collision safety of a hard-shell low-mass vehicle

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kaeser, R.; Walz, F.H.; Brunner, A.

1994-06-01

Low-mass vehicles and in particular low-mass electric vehicles as produced today in very small quantities are in general not designed for crashworthiness in collisions. Particular problems of compact low-mass cars are: reduced length of the car front, low mass compared to other vehicles, and heavy batteries in the case of an electric car. With the intention of studying design improvements, three frontal crash tests were run last year: the first one with a commercial, lightweight electric car; the second with a reinforced version of the same car; and the last one with a car based on a different structural designmore » with a `hard-shell` car body. Crash tests showed that the latter solution made better use of the small zone available for continuous energy absorption. The paper discusses further the problem of frontal collisions between vehicles of different weight and, in particular, the side collision. A side-collision test was run with the hard-shell vehicle following the ECE lateral-impact test procedure at 50 km/h and led to results for the EuroSIDI-dummy well below current injury tolerance criteria.« less

Collision safety of a hard-shell low-mass vehicle.

PubMed

Kaeser, R; Walz, F H; Brunner, A

1994-06-01

Low-mass vehicles and in particular low-mass electric vehicles as produced today in very small quantities are in general not designed for crashworthiness in collisions. Particular problems of compact low-mass cars are: reduced length of the car front, low mass compared to other vehicles, and heavy batteries in the case of an electric car. With the intention of studying design improvements, three frontal crash tests were run last year: the first one with a commercial, lightweight electric car; the second with a reinforced version of the same car; and the last one with a car based on a different structural design with a "hard-shell" car body. Crash tests showed that the latter solution made better use of the small zone available for continuous energy absorption. The paper discusses further the problem of frontal collisions between vehicles of different weight and, in particular, the side collision. A side-collision test was run with the hard-shell vehicle following the ECE lateral-impact test procedure at 50 km/h and led to results for the EuroSID1-dummy well below current injury tolerance criteria.

CAR-T cells targeting CLL-1 as an approach to treat acute myeloid leukemia.

PubMed

Wang, Jinghua; Chen, Siyu; Xiao, Wei; Li, Wende; Wang, Liang; Yang, Shuo; Wang, Weida; Xu, Liping; Liao, Shuangye; Liu, Wenjian; Wang, Yang; Liu, Nawei; Zhang, Jianeng; Xia, Xiaojun; Kang, Tiebang; Chen, Gong; Cai, Xiuyu; Yang, Han; Zhang, Xing; Lu, Yue; Zhou, Penghui

2018-01-10

Acute myeloid leukemia (AML) is one of the most common types of adult acute leukemia. Standard chemotherapies can induce complete remission in selected patients; however, a majority of patients eventually relapse and succumb to the disease. Thus, the development of novel therapeutics for AML is urgently needed. Human C-type lectin-like molecule-1 (CLL-1) is a type II transmembrane glycoprotein, and its expression is restricted to myeloid cells and the majority of AML blasts. Moreover, CLL-1 is expressed in leukemia stem cells (LSCs), but absent in hematopoietic stem cells (HSCs), which may provide a potential therapeutic target for AML treatment. We tested the expression of CLL-1 antigen on peripheral blood cells and bone marrow cells in healthy donor and AML patients. Then, we developed a chimeric antigen receptor (CAR) containing a CLL1-specific single-chain variable fragment, in combination with CD28, 4-1BB costimulatory domains, and CD3-ζ signaling domain. We further investigate the function of CLL-1 CAR-T cells. The CLL-1 CAR-T cells specifically lysed CLL-1 + cell lines as well as primary AML patient samples in vitro. Strong anti-leukemic activity was observed in vivo by using a xenograft model of disseminated AML. Importantly, CLL-1 + myeloid progenitor cells and mature myeloid cells were specifically eliminated by CLL-1 CAR-T cells, while normal HSCs were not targeted due to the lack of CLL-1 expression. CLL-1 CAR-T represents a promising immunotherapy for the treatment of AML.

Effect of Carnosine on Renal Function, Oxidation and Glycation Products in the Kidneys of High-Fat Diet/Streptozotocin-Induced Diabetic Rats.

PubMed

Fatih Aydın, Abdurrahman; Küçükgergin, Canan; Bingül, İlknur; Doğan-Ekici, Işın; Doğru-Abbasoğlu, Semra; Uysal, Müjdat

2017-05-01

High fat diet (HFD) and low dose of streptozotocin (STZ)-treated rats provide an animal model for type 2 Diabetes Mellitus (T2DM). Oxidative stress plays a role in the development of diabetic complications. Carnosine (CAR) has antioxidant and antiglycating properties. We investigated effects of CAR on renal function, oxidation and glycation products in HFD+STZ-rats. Rats were fed with HFD (60% of total calories from fat) for 4 weeks and then a single dose STZ (40 mg/kg; i.p.) was applied. Rats with blood glucose levels above 200 mg/dL were fed with HFD until the end of the 12 th week. CAR (250 mg/kg body weight; i.p.; 5 times a week) was administered to rats for the last 4 weeks. Glycated hemoglobin (HbA1c), glucose, lipids, and andrenal function tests in serum as well as reactive oxygen species, malondialdehyde, protein carbonyl, advanced oxidation protein products, advanced glycation end products (AGEs), antioxidant power, and antioxidant enzyme activities and their mRNA expressions in kidneys were determined. CAR treatment did not alter glucose and HbA1c, but it decreased serum lipids, creatinine, and urea levels in HFD+STZ rats. Oxidation products of lipids and proteins and AGEs levels decreased, but antioxidant enzyme activities and their mRNA expressions remained unchanged due to CAR treatment. Our results indicate that CAR treatment alleviated renal function and decreased accumulation of oxidation and glycation products in kidneys in HFD+STZ-rats. © Georg Thieme Verlag KG Stuttgart · New York.

Rail passenger equipment collision tests : analysis of structural measurements

DOT National Transportation Integrated Search

2000-11-01

A two-car full-scale collision test was conducted on April 4, 2000. Two coupled rail passenger cars impacted a rigid wall at 26 mph. The cars were instrumented with strain gauges, accelerometers, and string potentiometers, to measure the deformation ...

Repair of Budd Pioneer Coach car crush zones

DOT National Transportation Integrated Search

2007-05-01

The research team conducted a project to repair cars for use in a full-scale train-to-train collision test with crash energy management systems. The two cars had been damaged in previous dynamic tests. Several components required replacement, and som...

Responses of the Q6/Q6s ATD Positioned in Booster Seats in the Far-Side Seat Location of Side Impact Passenger Car and Sled Tests.

PubMed

Tylko, Suzanne; Bohman, Katarina; Bussières, Alain

2015-11-01

Passenger car side impact crash tests and sled tests were conducted to investigate the influence of booster seats, near-side occupant characteristics and vehicle interiors on the responses of the Q6/Q6s child ATD positioned in the rear, far-side seating location. Data from nine side impact sled tests simulating a EuroNCAP AEMD barrier test were analyzed with data obtained from 44 side impact crash tests. The crash tests included: FMVSS 214 and IIHS MDB, moving car-to-stationary car and moving car-to-moving car. A Q6 or prototype Q6s ATD was seated on the far-side, using a variety of low and high back booster seats. Head and chest responses were recorded and ATD motions were tracked with high-speed videos. The vehicle lateral accelerations resulting from MDB tests were characterized by a much earlier and more rapid rise to peak than in tests where the bullet was another car. The near-side seating position was occupied by a Hybrid III 10-year-old ATD in the sled tests, and a rear or front facing child restraint or a 5th percentile side impact ATD in the crash tests. Head impacts occurred more frequently in vehicles where a forward facing child restraint was present behind the driver seat for both the low and high back booster seats. Pretensioners were found to reduce lateral head displacements in all sled test configurations but the greatest reduction in lateral excursion was obtained with a high back booster seat secured with LATCH and tested in combination with pretensioners.

Lane Transit District "Curb Your Car" project : final report.

DOT National Transportation Integrated Search

2001-03-01

Lane Transit District has been active in utilizing transportation demand management techniques to encourage use of alternatives to the single occupant vehicle. In 1993, a Curb Your Car project indicated that participating employees use of al...

Lane Transit District "Curb Your Car" Project : final report

DOT National Transportation Integrated Search

2001-03-01

Lane Transit District has been active in utilizing transportation demand management techniques to encourage use of alternatives to the single occupant vehicle. In 1993, a Curb Your Car project indicated that participating employees' use of alternativ...

Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord

NASA Astrophysics Data System (ADS)

Shi, Yunzhou; Zhang, Delong; Huff, Terry B.; Wang, Xiaofei; Shi, Riyi; Xu, Xiao-Ming; Cheng, Ji-Xin

2011-10-01

In vivo imaging of white matter is important for the mechanistic understanding of demyelination and evaluation of remyelination therapies. Although white matter can be visualized by a strong coherent anti-Stokes Raman scattering (CARS) signal from axonal myelin, in vivo repetitive CARS imaging of the spinal cord remains a challenge due to complexities induced by the laminectomy surgery. We present a careful experimental design that enabled longitudinal CARS imaging of de- and remyelination at single axon level in live rats. In vivo CARS imaging of secretory phospholipase A2 induced myelin vesiculation, macrophage uptake of myelin debris, and spontaneous remyelination by Schwann cells are sequentially monitored over a 3 week period. Longitudinal visualization of de- and remyelination at a single axon level provides a novel platform for rational design of therapies aimed at promoting myelin plasticity and repair.

Side impact test and analyses of a DOT-111 tank car : final report.

DOT National Transportation Integrated Search

2015-10-01

Transportation Technology Center, Inc. conducted a side impact test on a DOT-111 tank car to evaluate the performance of the : tank car under dynamic impact conditions and to provide data for the verification and refinement of a computational model. ...

Rail-car impact tests with steel coil: collision dynamics

DOT National Transportation Integrated Search

2003-04-22

Two full-scale oblique grade-crossing impact tests were conducted in June 2002 to compare the crashworthiness performance of alternative corner post designs on rail passenger cab cars. On June 4, 2002 a cab car fitted with an end structure built to p...

Thoracic Injury Risk as a Function of Crash Severity – Car-to-car Side Impact Tests with WorldSID Compared to Real-life Crashes

PubMed Central

Sunnevång, Cecilia; Rosén, Erik; Boström, Ola; Lechelt, Ulf

2010-01-01

Side airbags reduce the risk of fatal injury by approximately 30%. Due to limited real-life data the risk reducing effect for serious injury has not yet been established. Since side airbags are mainly designed and validated for crash severities used in available test procedures little is known regarding the protective effect when severity increases. The objective of this study was to understand for which crash severities AIS3+ thorax occupant protection in car-to-car nearside collisions need to and can be improved. The aim was fulfilled by means of real life data, for older cars without side airbag, and a series of car-to-car tests performed with the WorldSID 50%-ile in modern and older cars at different impact speeds. The real life data showed that the risk of AIS3+ injury was highest for the thorax followed by the pelvis and head. For both non-senior and senior occupants, most thorax injuries were sustained at lateral delta-v from 20 km/h to 40 km/h. In this severity range, senior occupants were found to have approximately four times higher risk of thoracic injury than non-senior occupants. The crash tests at lateral impact speed 55 km/h (delta-v 32 km/h) confirmed the improved performance at severities represented in current legal and rating tests. The structural integrity of the modern car impacted at 70 km/h showed a potential for improved side impact protection by interior countermeasures. PMID:21050600

Thoracic Injury Risk as a Function of Crash Severity - Car-to-car Side Impact Tests with WorldSID Compared to Real-life Crashes.

PubMed

Sunnevång, Cecilia; Rosén, Erik; Boström, Ola; Lechelt, Ulf

2010-01-01

Side airbags reduce the risk of fatal injury by approximately 30%. Due to limited real-life data the risk reducing effect for serious injury has not yet been established. Since side airbags are mainly designed and validated for crash severities used in available test procedures little is known regarding the protective effect when severity increases.The objective of this study was to understand for which crash severities AIS3+ thorax occupant protection in car-to-car nearside collisions need to and can be improved. The aim was fulfilled by means of real life data, for older cars without side airbag, and a series of car-to-car tests performed with the WorldSID 50%-ile in modern and older cars at different impact speeds.The real life data showed that the risk of AIS3+ injury was highest for the thorax followed by the pelvis and head. For both non-senior and senior occupants, most thorax injuries were sustained at lateral delta-v from 20 km/h to 40 km/h. In this severity range, senior occupants were found to have approximately four times higher risk of thoracic injury than non-senior occupants. The crash tests at lateral impact speed 55 km/h (delta-v 32 km/h) confirmed the improved performance at severities represented in current legal and rating tests. The structural integrity of the modern car impacted at 70 km/h showed a potential for improved side impact protection by interior countermeasures.

Side impact test and analysis of a DOT-112 tank car.

DOT National Transportation Integrated Search

2016-12-01

As part of a program to improve transportation safety for tank cars, Transportation Technology Center, Inc. (TTCI) has conducted a side impact test on a DOT-112 tank car to evaluate the performance of the DOT-112 under dynamic impact conditions and t...

A revolution in preventing fatal craniovertebral junction injuries: lessons learned from the Head and Neck Support device in professional auto racing.

PubMed

Kaul, Anand; Abbas, Ahmed; Smith, Gabriel; Manjila, Sunil; Pace, Jonathan; Steinmetz, Michael

2016-12-01

Fatal craniovertebral junction (CVJ) injuries were the most common cause of death in high-speed motor sports prior to 2001. Following the death of a mutual friend and race car driver, Patrick Jacquemart (1946-1981), biomechanical engineer Dr. Robert Hubbard, along with race car driver and brother-in-law Jim Downing, developed the concept for the Head and Neck Support (HANS) device to prevent flexion-distraction injuries during high-velocity impact. Biomechanical testing showed that neck shear and loading forces experienced during collisions were 3 times the required amount for a catastrophic injury. Crash sled testing with and without the HANS device elucidated reductions in neck tension, neck compression, head acceleration, and chest acceleration experienced by dummies during high-energy crashes. Simultaneously, motor sports accidents such as Dale Earnhardt Sr.'s fatal crash in 2001 galvanized public opinion in favor of serious safety reform. Analysis of Earnhardt's accident demonstrated that his car's velocity parallel to the barrier was more than 150 miles per hour (mph), with deceleration upon impact of roughly 43 mph in a total of 0.08 seconds. After careful review, several major racing series such as the National Association for Stock Car Auto Racing (NASCAR) and Championship Auto Racing Team (CART) made major changes to ensure the safety of drivers at the turn of the 21st century. Since the rule requiring the HANS device in professional auto racing series was put in place, there has not been a single reported case of a fatal CVJ injury.

CAR T-cell therapy for glioblastoma: ready for the next round of clinical testing?

PubMed

Prinzing, Brooke L; Gottschalk, Stephen M; Krenciute, Giedre

2018-05-01

The outcome for patients with glioblastoma (GBM) remains poor, and there is an urgent need to develop novel therapeutic approaches. T cells genetically modified with chimeric antigen receptors (CARs) hold the promise to improve outcomes since they recognize and kill cells through different mechanisms than conventional therapeutics. Areas covered: This article reviews CAR design, tumor associated antigens expressed by GBMs that can be targeted with CAR T cells, preclinical and clinical studies conducted with CAR T cells, and genetic approaches to enhance their effector function. Expert commentary: While preclinical studies have highlighted the potent anti-GBM activity of CAR T cells, the initial foray of CAR T-cell therapies into the clinic resulted only in limited benefits for GBM patients. Additional genetic modification of CAR T cells has resulted in a significant increase in their anti-GBM activity in preclinical models. We are optimistic that clinical testing of these enhanced CAR T cells will be safe and result in improved anti-glioma activity in GBM patients.

[Novel therapy for malignant lymphoma: adoptive immuno-gene therapy using chimeric antigen receptor(CAR)-expressing T lymphocytes].

PubMed

Ozawa, Keiya

2014-03-01

Adoptive T-cell therapy using chimeric antigen receptor (CAR) technology is a novel approach to cancer immuno-gene therapy. CARs are hybrid proteins consisting of target-antigen-specific single-chain antibody fragment fused to intracellular T-cell activation domains (CD28 or CD137/CD3 zeta receptor). CAR-expressing engineered T lymphocytes can directly recognize and kill tumor cells in an HLA independent manner. In the United States, promising results have been obtained in the clinical trials of adoptive immuno-gene therapy using CD19-CAR-T lymphocytes for the treatment of refractory B-cell malignancies, including chronic lymphocytic leukemia (CLL) and acute lymphoblastic leukemia (ALL). In this review article, CD19-CAR-T gene therapy for refractory B-cell non-Hodgkin lymphoma is discussed.

CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex.

PubMed

Bae, Brian; Chen, James; Davis, Elizabeth; Leon, Katherine; Darst, Seth A; Campbell, Elizabeth A

2015-09-08

A key point to regulate gene expression is at transcription initiation, and activators play a major role. CarD, an essential activator in Mycobacterium tuberculosis, is found in many bacteria, including Thermus species, but absent in Escherichia coli. To delineate the molecular mechanism of CarD, we determined crystal structures of Thermus transcription initiation complexes containing CarD. The structures show CarD interacts with the unique DNA topology presented by the upstream double-stranded/single-stranded DNA junction of the transcription bubble. We confirm that our structures correspond to functional activation complexes, and extend our understanding of the role of a conserved CarD Trp residue that serves as a minor groove wedge, preventing collapse of the transcription bubble to stabilize the transcription initiation complex. Unlike E. coli RNAP, many bacterial RNAPs form unstable promoter complexes, explaining the need for CarD.

Lack of α2C-Adrenoceptor Results in Contrasting Phenotypes of Long Bones and Vertebra and Prevents the Thyrotoxicosis-Induced Osteopenia

PubMed Central

Cruz Grecco Teixeira, Marilia Bianca; Martins, Gisele Miyamura; Miranda-Rodrigues, Manuela; De Araújo, Iasmin Ferreira; Oliveira, Ricardo; Brum, Patrícia Chakur; Azevedo Gouveia, Cecilia Helena

2016-01-01

A series of studies have demonstrated that activation of the sympathetic nervous system (SNS) causes osteopenia via β2-adrenoceptor (β2-AR) signaling. However, in a recent study, we found an unexpected and generalized phenotype of high bone mass in female mice with chronic sympathetic hyperactivity, due to double gene inactivation of adrenoceptors that negatively regulate norepinephrine release, α2A-and α2C-AR (α2A/2C-AR-/-). These findings suggest that β2-AR is not the single adrenoceptor involved in bone turnover regulation and show that α2-AR signaling may also mediate the SNS actions in the skeleton. In addition, we found that α2A/2C-AR-/- animals are resistant to the thyrotoxicosis-induced osteopenia, suggesting that thyroid hormone (TH), when in supraphysiological levels, interacts with the SNS to control bone mass and structure, and that this interaction may also involve α2-AR signaling. In the present study, to further investigate these hypotheses and to discriminate the roles of α2-AR subtypes, we have evaluated the bone phenotype of mice with the single gene inactivation of α2C-AR subtype, which mRNA expression was previously shown to be down regulated by triiodothyronine (T3). A cohort of 30 day-old female α2CAR-/- mice and their wild-type (WT) controls were treated with a supraphysiological dose of T3 for 30 or 90 days, which induced a thyrotoxic state in both mouse lineages. The micro-computed tomographic (μCT) analysis showed that α2C-AR-/- mice present lower trabecular bone volume (BV/TV) and number (Tb.N), and increased trabecular separation (Tb.Sp) in the femur compared with WT mice; which was accompanied by decreased bone strength (determined by the three-point bending test) in the femur and tibia. The opposite was observed in the vertebra, where α2C-AR-/- mice show increased BV/TV, Tb.N and trabecular thickness (Tb.Th), and decreased Tb.Sp, compared with WT animals. In spite of the contrasting bone phenotypes of the femur and L5, thyrotoxicosis negatively regulated most of the micro architectural features of the trabecular bone in both skeletal sites of WT, but not of α2C-AR-/- mice. T3 treatment also decreased biomechanical properties (maximum load and ultimate load) in the femur and tibia of WT, but not of knockout mice. The mRNA expression of osteocalcin, a marker of mature osteoblasts, and tartrate-resistant acid phosphatase, which is expressed by osteoclasts and is involved in collagen degradation, was increased by T3 treatment only in WT, and not in α2C-AR-/- mice. Altogether, these findings suggest that α2C-AR subtype mediates the effects of the SNS in the bone in a skeletal site-dependent manner, and that thyrotoxicosis depends on α2C-AR signaling to promote bone loss, which sustains the hypothesis of a TH-SNS interaction to modulate bone remodeling and structure. PMID:26815679

Lack of α2C-Adrenoceptor Results in Contrasting Phenotypes of Long Bones and Vertebra and Prevents the Thyrotoxicosis-Induced Osteopenia.

PubMed

Cruz Grecco Teixeira, Marilia Bianca; Martins, Gisele Miyamura; Miranda-Rodrigues, Manuela; De Araújo, Iasmin Ferreira; Oliveira, Ricardo; Brum, Patrícia Chakur; Azevedo Gouveia, Cecilia Helena

2016-01-01

A series of studies have demonstrated that activation of the sympathetic nervous system (SNS) causes osteopenia via β2-adrenoceptor (β2-AR) signaling. However, in a recent study, we found an unexpected and generalized phenotype of high bone mass in female mice with chronic sympathetic hyperactivity, due to double gene inactivation of adrenoceptors that negatively regulate norepinephrine release, α2A-and α2C-AR (α2A/2C-AR-/-). These findings suggest that β2-AR is not the single adrenoceptor involved in bone turnover regulation and show that α2-AR signaling may also mediate the SNS actions in the skeleton. In addition, we found that α2A/2C-AR-/- animals are resistant to the thyrotoxicosis-induced osteopenia, suggesting that thyroid hormone (TH), when in supraphysiological levels, interacts with the SNS to control bone mass and structure, and that this interaction may also involve α2-AR signaling. In the present study, to further investigate these hypotheses and to discriminate the roles of α2-AR subtypes, we have evaluated the bone phenotype of mice with the single gene inactivation of α2C-AR subtype, which mRNA expression was previously shown to be down regulated by triiodothyronine (T3). A cohort of 30 day-old female α2CAR-/- mice and their wild-type (WT) controls were treated with a supraphysiological dose of T3 for 30 or 90 days, which induced a thyrotoxic state in both mouse lineages. The micro-computed tomographic (μCT) analysis showed that α2C-AR-/- mice present lower trabecular bone volume (BV/TV) and number (Tb.N), and increased trabecular separation (Tb.Sp) in the femur compared with WT mice; which was accompanied by decreased bone strength (determined by the three-point bending test) in the femur and tibia. The opposite was observed in the vertebra, where α2C-AR-/- mice show increased BV/TV, Tb.N and trabecular thickness (Tb.Th), and decreased Tb.Sp, compared with WT animals. In spite of the contrasting bone phenotypes of the femur and L5, thyrotoxicosis negatively regulated most of the micro architectural features of the trabecular bone in both skeletal sites of WT, but not of α2C-AR-/- mice. T3 treatment also decreased biomechanical properties (maximum load and ultimate load) in the femur and tibia of WT, but not of knockout mice. The mRNA expression of osteocalcin, a marker of mature osteoblasts, and tartrate-resistant acid phosphatase, which is expressed by osteoclasts and is involved in collagen degradation, was increased by T3 treatment only in WT, and not in α2C-AR-/- mice. Altogether, these findings suggest that α2C-AR subtype mediates the effects of the SNS in the bone in a skeletal site-dependent manner, and that thyrotoxicosis depends on α2C-AR signaling to promote bone loss, which sustains the hypothesis of a TH-SNS interaction to modulate bone remodeling and structure.

General Vehicle Test Plan (GVTP) for Urban Rail Transit Cars

DOT National Transportation Integrated Search

1977-09-01

The General Vehicle Test Plan provides a system for general vehicle testing and for documenting and utilizing data and information in the testing of urban rail transit cars. Test procedures are defined for nine categories: (1) Performance; (2) Power ...

Study concerning the loads over driver's chests in car crashes with cars of the same or different generation

NASA Astrophysics Data System (ADS)

Ispas, N.; Năstăsoiu, M.

2016-08-01

Reducing occupant injuries for cars involves in traffic accidents is a main target of today cars designers. Known as active or passive safety, many technological solutions were developing over the time for an actual better car's occupant safety. In the real world, in traffic accidents are often involved cars from different generations with various safety historical solutions. The main aim of these papers are to quantify the influences over the car driver chest loads in cases of same or different generation of cars involved in side car crashes. Both same and different cars generations were used for the study. Other goal of the paper was the study of in time loads conformity for diver's chests from both cars involved in crash. The paper's experimental results were obtained by support of DSD, Dr. Steffan Datentechnik GmbH - Linz, Austria. The described tests were performed in full test facility of DSD Linz, in “Easter 2015 PC-Crash Seminar”. In all crashes we obtaining results from both dummy placed in impacted and hits car. The novelty of the paper are the comparisons of data set from each of driver (dummy) of two cars involved in each of six experimental crashes. Another novelty of this paper consists in possibilities to analyse the influences of structural historical cars solutions over deformation and loads in cases of traffic accidents involved. Paper's conclusions can be future used for car passive safety improvement.

A compound chimeric antigen receptor strategy for targeting multiple myeloma.

PubMed

Chen, K H; Wada, M; Pinz, K G; Liu, H; Shuai, X; Chen, X; Yan, L E; Petrov, J C; Salman, H; Senzel, L; Leung, E L H; Jiang, X; Ma, Y

2018-02-01

Current clinical outcomes using chimeric-antigen receptors (CARs) against multiple myeloma show promise in the eradication of bulk disease. However, these anti-BCMA (CD269) CARs observe relapse as a common phenomenon after treatment due to the reemergence of either antigen-positive or -negative cells. Hence, the development of improvements in CAR design to target antigen loss and increase effector cell persistency represents a critical need. Here, we report on the anti-tumor activity of a CAR T-cell possessing two complete and independent CAR receptors against the multiple myeloma antigens BCMA and CS1. We determined that the resulting compound CAR (cCAR) T-cell possesses consistent, potent and directed cytotoxicity against each target antigen population. Using multiple mouse models of myeloma and mixed cell populations, we are further able to show superior in vivo survival by directed cytotoxicity against multiple populations compared to a single-expressing CAR T-cell. These findings indicate that compound targeting of BCMA and CS1 on myeloma cells can potentially be an effective strategy for augmenting the response against myeloma bulk disease and for initiation of broader coverage CAR therapy.

49 CFR 238.313 - Class I brake test.

Code of Federal Regulations, 2013 CFR

2013-10-01

... occurs first, that the train remains in continuous passenger service. (c) Each passenger car and each... performed on the car within the previous calendar day and the car has not been disconnected from a source of... applied on each car in the train until a release of the brakes has been initiated on each car in response...

49 CFR 238.313 - Class I brake test.

Code of Federal Regulations, 2014 CFR

2014-10-01

... occurs first, that the train remains in continuous passenger service. (c) Each passenger car and each... performed on the car within the previous calendar day and the car has not been disconnected from a source of... applied on each car in the train until a release of the brakes has been initiated on each car in response...

49 CFR 238.313 - Class I brake test.

Code of Federal Regulations, 2012 CFR

2012-10-01

... occurs first, that the train remains in continuous passenger service. (c) Each passenger car and each... performed on the car within the previous calendar day and the car has not been disconnected from a source of... applied on each car in the train until a release of the brakes has been initiated on each car in response...

49 CFR 238.313 - Class I brake test.

Code of Federal Regulations, 2011 CFR

2011-10-01

... occurs first, that the train remains in continuous passenger service. (c) Each passenger car and each... performed on the car within the previous calendar day and the car has not been disconnected from a source of... applied on each car in the train until a release of the brakes has been initiated on each car in response...

49 CFR 238.313 - Class I brake test.

Code of Federal Regulations, 2010 CFR

2010-10-01

... occurs first, that the train remains in continuous passenger service. (c) Each passenger car and each... performed on the car within the previous calendar day and the car has not been disconnected from a source of... applied on each car in the train until a release of the brakes has been initiated on each car in response...

Preliminary analysis of a downsized advanced gas-turbine engine in a subcompact car

NASA Technical Reports Server (NTRS)

Klann, J. L.; Johnsen, R. L.

1982-01-01

Relative fuel economy advantages exist for a ceramic turbine engine when it is downsized for a small car were investigated. A 75 kW (100 hp) single shaft engine under development was analytically downsized to 37 kW (50 hp) and analyzed with a metal belt continuously variable transmission in a synthesized car. With gasoline, a 25% advantage was calculated over that of a current spark ignition engine, scaled to the same power, using the same transmission and car. With diesel fuel, a 21% advantage was calculated over that of a similar diesel engine vehicle.

CARS applications to combustion diagnostics

NASA Astrophysics Data System (ADS)

Eckbreth, Alan C.

1986-01-01

Attention is given to broadband or multiplex CARS of combustion processes, using pulsed lasers whose intensity is sufficiently great for instantaneous measurement of medium properties. This permits probability density functions to be assembled from a series of single-pulse measurements, on the basis of which the true parameter average and the magnitude of the turbulent fluctuations can be ascertained. CARS measurements have been conducted along these lines in diesel engines, gas turbine combustors, scramjets, and solid rocket propellants.

49 CFR 232.607 - Inspection and testing requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... car in a train has traveled since receiving a Class I brake test by a qualified mechanical inspector... restricts access to the train and provides sufficient security to deter vandalism. (c) Cars added en route. (1) Each freight car equipped with an ECP brake system that is added to a freight train operating in...

49 CFR 232.607 - Inspection and testing requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... car in a train has traveled since receiving a Class I brake test by a qualified mechanical inspector... restricts access to the train and provides sufficient security to deter vandalism. (c) Cars added en route. (1) Each freight car equipped with an ECP brake system that is added to a freight train operating in...

49 CFR 232.607 - Inspection and testing requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... car in a train has traveled since receiving a Class I brake test by a qualified mechanical inspector... restricts access to the train and provides sufficient security to deter vandalism. (c) Cars added en route. (1) Each freight car equipped with an ECP brake system that is added to a freight train operating in...

49 CFR 232.607 - Inspection and testing requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... car in a train has traveled since receiving a Class I brake test by a qualified mechanical inspector... restricts access to the train and provides sufficient security to deter vandalism. (c) Cars added en route. (1) Each freight car equipped with an ECP brake system that is added to a freight train operating in...

49 CFR 232.607 - Inspection and testing requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... car in a train has traveled since receiving a Class I brake test by a qualified mechanical inspector... restricts access to the train and provides sufficient security to deter vandalism. (c) Cars added en route. (1) Each freight car equipped with an ECP brake system that is added to a freight train operating in...

Study of DC Circuit Breaker of H2-N2 Mixture Gas for High Voltage

NASA Astrophysics Data System (ADS)

Shiba, Yuji; Morishita, Yukinaga; Kaneko, Shuhei; Okabe, Shigemitsu; Mizoguchi, Hitoshi; Yanabu, Satoru

Global warming caused by CO2 etc. is a field where the concern is very high. Especially, automobile emissions are problem for it. Therefore, the hybrid car is widely development and used recently. Hybrid car used electric power and gasoline. So, the car reduces CO2. Hybrid car has engine and motor. To rotate the motor, hybrid car has battery. This battery is large capacity. Therefore, the relay should interrupt high DC current for the switch of the motor and the engine. So, hybrid car used hydrogen gas filling relay We studied interruption test for the research of a basic characteristic of hydrogen gas. DC current has not current zero point. So, it is necessary to make the current zero by high arc voltage and forcible current zero point. The loss coefficient and arc voltage of hydrogen is high. Therefore, we studied interruption test for used high arc voltage. We studied interruption test and dielectric breakdown test of air, pure Hydrogen, and Hydrogen- nitrogen mixture gas. As a result, we realized H2-N2(80%-20%) is the best gas.

The effectiveness of lane departure warning systems-A reduction in real-world passenger car injury crashes.

PubMed

Sternlund, Simon; Strandroth, Johan; Rizzi, Matteo; Lie, Anders; Tingvall, Claes

2017-02-17

The objective of this study was to estimate the safety benefits of in vehicle lane departure warning (LDW) and lane keeping aid (LKA) systems in reducing relevant real-world passenger car injury crashes. The study used an induced exposure method, where LDW/LKA-sensitive and nonsensitive crashes were compared for Volvo passenger cars equipped with and without LDW/LKA systems. These crashes were matched by car make, model, model year, and technical equipment; that is, low-speed autonomous emergency braking (AEB) called City Safety (CS). The data were extracted from the Swedish Traffic Accident Data Acquisition database (STRADA) and consisted of 1,853 driver injury crashes that involved 146 LDW-equipped cars, 11 LKA-equipped cars, and 1,696 cars without LDW/LKA systems. The analysis showed a positive effect of the LDW/LKA systems in reducing lane departure crashes. The LDW/LKA systems were estimated to reduce head-on and single-vehicle injury crashes on Swedish roads with speed limits between 70 and 120 km/h and with dry or wet road surfaces (i.e., not covered by ice or snow) by 53% with a lower limit of 11% (95% confidence interval [CI]). This reduction corresponded to a reduction of 30% with a lower limit of 6% (95% CI) for all head-on and single-vehicle driver injury crashes (including all speed limits and all road surface conditions). LDW/LKA systems were estimated to lower the driver injury risk in crash types that the systems are designed to prevent; that is, head-on and single-vehicle crashes. Though these are important findings, they were based on a small data set. Therefore, further research is desirable to evaluate the effectiveness of LDW/LKA systems under real-world conditions and to differentiate the effectiveness between technical solutions (i.e., LDW and LKA) proposed by different manufacturers.

Local delivery of interleukin-12 using T cells targeting VEGF receptor-2 eradicates multiple vascularized tumors in mice.

PubMed

Chinnasamy, Dhanalakshmi; Yu, Zhiya; Kerkar, Sid P; Zhang, Ling; Morgan, Richard A; Restifo, Nicholas P; Rosenberg, Steven A

2012-03-15

We investigated the feasibility of delivering the proinflammatory cytokine interleukin (IL)-12 into tumor using T cells genetically engineered to express a chimeric antigen receptor (CAR) against the VEGF receptor-2 (VEGFR-2). Two different strains of mice bearing five different established subcutaneous tumors were treated with syngeneic T cells cotransduced with an anti-VEGFR-2 CAR and a constitutively expressed single-chain murine IL-12 or an inducible IL-12 gene after host lymphodepletion. Tumor regression, survival of mice, and persistence of the transferred cells were evaluated. Adoptive transfer of syngeneic T cells cotransduced with an anti-VEGFR-2 CAR and a constitutively expressing single-chain IL-12 resulted in the regression of five different established tumors of different histologies without the need for IL-2 administration. T cells transduced with either anti-VEGFR-2 CAR or single-chain IL-12 alone did not alter the tumor growth indicating that both of them had to be expressed in the same cell to mediate tumor regression. Anti-VEGFR-2 CAR and IL-12-cotransduced T cells infiltrated the tumors, expanded, and persisted for prolonged periods. The antitumor effect did not require the presence of host T and B cells but was dependent on host IL-12R-expressing cells. The anti-VEGFR-2 CAR changed the immunosuppressive tumor environment by altering/reducing both the systemic and the intratumoral CD11b(+)Gr1(+) myeloid suppressor cell subsets that expressed VEGFR-2. These results suggest that targeted delivery of IL-12 into the tumor environment with T cells redirected against VEGFR-2 is a promising approach for treating patients with a variety of solid tumor types.

A Pilot Study on the Combination of Applied Behavior Analysis and Bumetanide Treatment for Children with Autism.

PubMed

Du, Lin; Shan, Ling; Wang, Bing; Li, Honghua; Xu, Zhida; Staal, Wouter G; Jia, Feiyong

2015-09-01

The purpose of this study was to investigate the therapeutic effects of combined bumetanide and applied behavior analysis (ABA) treatment in children with autism. Sixty children diagnosed with autism according to the International Classification of Diseases, Tenth Revision (ICD-10) criteria (mean age of 4.5 years) were randomly divided into two groups: A single treatment group (n=28) and a combined treatment group (n=32). The combined treatment group received ABA training combined with oral bumetanide (0.5 mg twice a day). The single treatment group received ABA training only. Autism symptoms were evaluated with the Autism Behavior Checklist (ABC) and the Childhood Autism Rating Scale (CARS), whereas severity of disease (SI) and global improvement (GI) were measured with the Clinical Global Impressions (CGI). Assessment of ABC, CARS, and CGI was performed immediately before and 3 months after initiation of the treatment(s). Prior to intervention(s) no statistically significant differences in scores on the ABC, CARS, SI, or GI were found between the two groups. Total scores of the ABC, CARS, and SI were decreased in both groups after 3 months (p<0.05) compared with the scores prior to treatment. The total scores of the ABC and the CGI were significantly (p<0.05) lower in the combined treatment group than in the single treatment group. Although the total and item scores of the CARS in the combined treatment group were lower than in the single treatment group after a 3 month intervention, they did not reach statistical significance. No adverse effects of bumetanide were observed. Treatment with bumetanide combined with ABA training may result in a better outcome in children with autism than ABA training alone.

Developing single-laser sources for multimodal coherent anti-Stokes Raman scattering microscopy

NASA Astrophysics Data System (ADS)

Pegoraro, Adrian Frank

Coherent anti-Stokes Raman scattering (CARS) microscopy has developed rapidly and is opening the door to new types of experiments. This work describes the development of new laser sources for CARS microscopy and their use for different applications. It is specifically focused on multimodal nonlinear optical microscopy—the simultaneous combination of different imaging techniques. This allows us to address a diverse range of applications, such as the study of biomaterials, fluid inclusions, atherosclerosis, hepatitis C infection in cells, and ice formation in cells. For these applications new laser sources are developed that allow for practical multimodal imaging. For example, it is shown that using a single Ti:sapphire oscillator with a photonic crystal fiber, it is possible to develop a versatile multimodal imaging system using optimally chirped laser pulses. This system can perform simultaneous two photon excited fluorescence, second harmonic generation, and CARS microscopy. The versatility of the system is further demonstrated by showing that it is possible to probe different Raman modes using CARS microscopy simply by changing a time delay between the excitation beams. Using optimally chirped pulses also enables further simplification of the laser system required by using a single fiber laser combined with nonlinear optical fibers to perform effective multimodal imaging. While these sources are useful for practical multimodal imaging, it is believed that for further improvements in CARS microscopy sensitivity, new excitation schemes are necessary. This has led to the design of a new, high power, extended cavity oscillator that should be capable of implementing new excitation schemes for CARS microscopy as well as other techniques. Our interest in multimodal imaging has led us to other areas of research as well. For example, a fiber-coupling scheme for signal collection in the forward direction is demonstrated that allows for fluorescence lifetime imaging without significant temporal distortion. Also highlighted is an imaging artifact that is unique to CARS microscopy that can alter image interpretation, especially when using multimodal imaging. By combining expertise in nonlinear optics, laser development, fiber optics, and microscopy, we have developed systems and techniques that will be of benefit for multimodal CARS microscopy.

Comparison between Euro NCAP test results and real-world crash data.

PubMed

Kullgren, Anders; Lie, Anders; Tingvall, Claes

2010-12-01

The objective of this study was 2-fold: first, to compare Euro NCAP safety ratings of cars with those published by the Folksam real-world injury ratings; and second, to compare injury risk measures between Euro NCAP 2 and 5 Star cars with real-world injury and disability outcomes using police and insurance injury data. Car models were grouped according to the Euro NCAP star rating scores. Folksam risk of injury ratings come from statistical analysis of real-world crashes using police and insurance databases. The paired comparison method using 2-car crashes was used to control for crash speed and the mass differences between cars of different weights were normalized. For all comparisons, 5-star rated Euro NCAP cars were found to have a lower risk of injury compared to 2-star rated cars (5-star cars were 10% ± 2.5% lower risk than 2-star cars). For fatal and serious injuries, the difference was 23 ± 8 percent, and for fatal injuries alone the difference was 68 ± 32 percent. By comparison, the Folksam 5-star rated cars had a relative risk of 0.020 ± 0.0024, whereas 2-star rated car risk was 0.028 ± 0.0016, corresponding to a 27 percent difference in risk between 5- and 2-star cars. Good correlation was found between Euro NCAP test results and real-world injury outcomes. The largest difference in injury risk between 2- and 5-star rated cars in Euro NCAP was found for risk of fatality, confirming that car manufacturers have focused their safety performance on serious crash outcomes. In addition, Euro NCAP crash tests were shown to be highly correlated with serious crash performance, confirming their relevance for evaluating real-world crash performance. Good concordance was found between Euro NCAP and Folksam real-world crash and injury ratings.

Track/train dynamics test procedure transfer function test

NASA Technical Reports Server (NTRS)

Vigil, R. A.

1975-01-01

A transfer function vibration test was made on an 80 ton open hopper freight car in an effort to obtain validation data on the car's nonlinear elastic model. Test configuration, handling, test facilities, test operations, and data acquisition/reduction activities necessary to meet the conditions of test requirements are given.

Over-the-road testing of the instrumented tank car : a load environment study.

DOT National Transportation Integrated Search

2010-05-01

Fractures have been observed on stub sill tank cars for many years. Undetected and unattended, these fractures can develop into a variety of tank car failures. While tank car ruptures are relatively rare, the potential for a catastrophic HAZMAT relea...

Passenger train emergency systems : single-level commuter rail car egress experiments

DOT National Transportation Integrated Search

2015-04-01

Under FRA sponsorship, a series of three experimental egress trials was conducted in 2005 and 2006 to obtain human factors data relating to the amount of time necessary for individuals to exit from a passenger rail car. This final report describes th...

36 CFR 1192.111 - General.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false General. 1192.111 Section 1192.111 Parks, Forests, and Public Property ARCHITECTURAL AND TRANSPORTATION BARRIERS COMPLIANCE BOARD... rail passenger coaches and food service cars (other than single-level dining cars) shall comply with...

36 CFR 1192.111 - General.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false General. 1192.111 Section 1192.111 Parks, Forests, and Public Property ARCHITECTURAL AND TRANSPORTATION BARRIERS COMPLIANCE BOARD... rail passenger coaches and food service cars (other than single-level dining cars) shall comply with...

Quantitative identification of chemical compounds by dual-soliton based coherent anti-Stokes Raman scattering spectroscopy

NASA Astrophysics Data System (ADS)

Chen, Kun; Wu, Tao; Li, Yan; Wei, Haoyun

2017-12-01

Coherent anti-Stokes Raman scattering (CARS) is a powerful nonlinear spectroscopy technique that is rapidly gaining recognition of different molecules. Unfortunately, molecular concentration information is generally not immediately accessible from the raw CARS signal due to the nonresonant background. In addition, mainstream biomedical applications of CARS are currently hampered by a complex and bulky excitation setup. Here, we establish a dual-soliton Stokes based CARS spectroscopy scheme capable of quantifying the sample molecular, using a single fiber laser. This dual-soliton CARS scheme takes advantage of a differential configuration to achieve efficient suppression of nonresonant background and therefore allows extraction of quantitative composition information. Besides, our all-fiber based excitation source can probe the most fingerprint region (1100-1800 cm-1) with a spectral resolution of 15 cm-1 under the spectral focusing mechanism, where is considerably more information contained throughout an entire spectrum than at just a single frequency within that spectrum. Systematic studies of the scope of application and several fundamental aspects are discussed. Quantitative capability is further experimentally demonstrated through the determination of oleic acid concentration based on the linear dependence of signal on different Raman vibration bands.

Towards Single-Shot Detection of Bacterial Endospores via Coherent Raman Spectroscopy

NASA Astrophysics Data System (ADS)

Pestov, Dmitry; Wang, Xi; Ariunbold, Gombojav; Murawski, Robert; Sautenkov, Vladimir; Sokolov, Alexei; Scully, Marlan

2007-10-01

Recent advances in coherent anti-Stokes Raman scattering (CARS) spectroscopy hold exciting promise to make the most out of now readily available ultrafast laser sources. Techniques have been devised to mitigate the nonresonant four-wave-mixing in favor of informative Raman-resonant signal. In particular, a hybrid technique for CARS (see Science 316, 265 (2007)) brings together the advantages of coherent broadband pump-Stokes excitation of molecular vibrations and their time-delayed but frequency-resolved probing via a spectrally narrowed and shaped laser pulse. We apply this technique to the problem of real-time detection of warfare bioagents and report single-shot acquisition of a distinct CARS spectrum from a small volume of B. subtilis endospores (˜10^4 spores), a harmless surrogate for B. anthracis. We study the dependence of the CARS signal on the energy of the ultrashort preparation pulses and find the limit on the pulse energy fluence (˜0.2 J/cm^2), imposed by the laser-induced damage of the spores.

Driving CAR T-cells forward.

PubMed

Jackson, Hollie J; Rafiq, Sarwish; Brentjens, Renier J

2016-06-01

The engineered expression of chimeric antigen receptors (CARs) on the surface of T cells enables the redirection of T-cell specificity. Early clinical trials using CAR T cells for the treatment of patients with cancer showed modest results, but the impressive outcomes of several trials of CD19-targeted CAR T cells in the treatment of patients with B-cell malignancies have generated an increased enthusiasm for this approach. Important lessons have been derived from clinical trials of CD19-specific CAR T cells, and ongoing clinical trials are testing CAR designs directed at novel targets involved in haematological and solid malignancies. In this Review, we discuss these trials and present strategies that can increase the antitumour efficacy and safety of CAR T-cell therapy. Given the fast-moving nature of this field, we only discuss studies with direct translational application currently or soon-to-be tested in the clinical setting.

Driving CAR T-cells forward

PubMed Central

Jackson, Hollie J.; Rafiq, Sarwish; Brentjens, Renier J.

2017-01-01

The engineered expression of chimeric antigen receptors (CARs) on the surface of T cells enables the redirection of T-cell specificity. Early clinical trials using CAR T cells for the treatment of patients with cancer showed modest results, but the impressive outcomes of several trials of CD19-targeted CAR T cells in the treatment of patients with B-cell malignancies have generated an increased enthusiasm for this approach. Important lessons have been derived from clinical trials of CD19-specific CAR T cells, and ongoing clinical trials are testing CAR designs directed at novel targets involved in haematological and solid malignancies. In this Review, we discuss these trials and present strategies that can increase the antitumour efficacy and safety of CAR T-cell therapy. Given the fast-moving nature of this field, we only discuss studies with direct translational application currently or soon-to-be tested in the clinical setting. PMID:27000958

A comparative study of the elemental composition of the exhaust emissions of cars powered by liquefied petroleum gas and unleaded petrol

NASA Astrophysics Data System (ADS)

Lim, McKenzie C. H.; Ayoko, Godwin A.; Morawska, Lidia; Ristovski, Zoran D.; Jayaratne, E. Rohan; Kokot, Serge

Elements emitted from the exhausts of new Ford Falcon Forte cars powered by unleaded petrol (ULP) and liquefied petroleum gas (LPG) were measured on a chassis dynamometer. The measurements were carried out in February, June and August 2001, and at two steady state driving conditions (60 and 80 km h -1). Thirty seven elements were quantified in the exhaust samples by inductively coupled plasma mass spectrometry (ICPMS). The total emission factors of the elements from the exhausts of ULP cars were higher than those of LPG cars at both engine speeds even though high variability in the exhaust emissions from different cars was noted. The effect of the operating conditions such as mileage of the cars, engine speed, fuel and lubricating oil compositions on the emissions was studied. To investigate the effects of these conditions, multivariate data analysis methods were employed including exploratory principal component analysis (PCA), and the multi-criteria decision making methods (MCDM), preference ranking organization method for enrichment evaluation (PROMETHEE) and geometrical analysis for interactive aid (GAIA), for ranking the cars on the basis of the emission factors of the elements. PCA biplot of the complete data matrix showed a clear discrimination of the February, June and August emission test results. In addition, (i) platinum group elements (PGE) emissions were separated from each other in the three different clusters viz. Pt with February, Pd with June and Rh with August; (ii) the motor oil related elements, Zn and P, were particularly associated with the June and August tests (these vectors were also grouped with V, Al and Cu); and (iii) highest emissions of most major elements were associated with the August test after the cars have recorded their highest mileage. Extensive analysis with the aid of the MCDM ranking methods demonstrated clearly that cars powered by LPG outperform those powered by ULP. In general, cars tested in June perform better than those tested in August, which suggested that mileage was the key criterion of car performance on the basis of elemental emission factors.

Simultaneous observation of rotational coherent Stokes Raman scattering and coherent anti-Stokes Raman scattering in air and nitrogen

NASA Technical Reports Server (NTRS)

Snow, J. B.; Chang, R. K.; Zheng, J. B.; Leipertz, A.

1983-01-01

Rotational coherent Stokes Raman scattering (CSRS) and coherent anti-Stokes Raman scattering (CARS) in air and in nitrogen were observed simultaneously by using broadband generation and detection. In the broadband technique used, the entire CARS and CSRS spectrum was generated in a single laser pulse; the CSRS and CARS signals were dispersed by a spectrograph and detected simultaneously by an optical multichannel analyzer. A three-dimensional phase-matching geometry was used to achieve spatial resolution of the CSRS and CARS beams from the input beams. Under resonant conditions, similar experiments may provide a means of investigating the possible interaction between the CSRS and CARS processes in driving the rotational levels.

40 CFR Appendix III to Part 600 - Sample Fuel Economy Label Calculation

Code of Federal Regulations, 2011 CFR

2011-07-01

... miles per gallon. Note that the car line of the test vehicle using a given engine makes no difference... engine. These four car lines are: Ajax Boredom III Dodo Castor (Station Wagon) A. A car line is defined... different car line than the normal Castor car line made up of sedans, coupes, etc. B. The engine considered...

40 CFR Appendix III to Part 600 - Sample Fuel Economy Label Calculation

Code of Federal Regulations, 2014 CFR

2014-07-01

... miles per gallon. Note that the car line of the test vehicle using a given engine makes no difference... engine. These four car lines are: Ajax Boredom III Dodo Castor (Station Wagon) A. A car line is defined... different car line than the normal Castor car line made up of sedans, coupes, etc. B. The engine considered...

40 CFR Appendix III to Part 600 - Sample Fuel Economy Label Calculation

Code of Federal Regulations, 2013 CFR

2013-07-01

... miles per gallon. Note that the car line of the test vehicle using a given engine makes no difference... engine. These four car lines are: Ajax Boredom III Dodo Castor (Station Wagon) A. A car line is defined... different car line than the normal Castor car line made up of sedans, coupes, etc. B. The engine considered...

40 CFR Appendix III to Part 600 - Sample Fuel Economy Label Calculation

Code of Federal Regulations, 2012 CFR

2012-07-01

... miles per gallon. Note that the car line of the test vehicle using a given engine makes no difference... engine. These four car lines are: Ajax Boredom III Dodo Castor (Station Wagon) A. A car line is defined... different car line than the normal Castor car line made up of sedans, coupes, etc. B. The engine considered...

Engineering Tests for Energy Storage Cars at the Transportation Test Center : Volume 3. Noise Tests.

DOT National Transportation Integrated Search

1977-05-01

The primary purpose of the tests documented herein was to demonstrate the principles and feasibility of an energy storage type propulsion system, and its adaptability to an existing car design. The test program comprised four phases of tests on two N...

Engineering Tests for Energy Storage Cars at the Transportation Test Center : Volume 1. Program Description and Test Summary.

DOT National Transportation Integrated Search

1977-05-01

The primary purpose of the tests documented herein was to demonstrate the principles and feasibility of an energy storage type propulsion system, and its adaptability to an existing car design. The test program comprised four phases of tests on two N...

Aerodynamic Improvements to Cargo Carrying Rail Cars due to Roof Modifications

NASA Astrophysics Data System (ADS)

Condie, Robert; Maynes, Daniel

2012-11-01

The aerodynamic drag associated with the transport of commodities by rail is becoming increasingly important as the cost of diesel fuel increases. We provide an assessment of the influence of the roof structure on aerodynamic performance of two dissimilar rail cars, namely automobile carrying cars and coal carrying cars. Currently, the roof material for automobile carrying rail cars is corrugated steel, with the corrugation aligned perpendicular to the direction of travel. Coal cars are currently left uncovered for loading convenience and on the return leg from the power plant are empty. Aerodynamic drag data have been obtained through wind tunnel testing on 1/29 scale models to understand the savings that may be realized by judicious modification to the tops of both these car types. For the automobile-carrying cars, testing is performed for the corrugated and smooth roof configurations. This modification alone has the potential of reducing the car drag coefficient by nominally 25%. A broader study is performed for the coal cars, with data being acquired for coal filled models, empty models, and several cover prototype configurations. The results reveal that implementation of a cover may yield reductions in the aerodynamic drag for both coal filled (nominally 7%) and empty coal cars (nominally 30%).

49 CFR 210.29 - Operation standards (moving locomotives and rail cars).

Code of Federal Regulations, 2011 CFR

2011-10-01

... cars). 210.29 Section 210.29 Transportation Other Regulations Relating to Transportation (Continued... REGULATIONS Inspection and Testing § 210.29 Operation standards (moving locomotives and rail cars). The operation standards for the noise emission levels of moving locomotives, rail cars, or consists of...

49 CFR 210.29 - Operation standards (moving locomotives and rail cars).

Code of Federal Regulations, 2010 CFR

2010-10-01

... cars). 210.29 Section 210.29 Transportation Other Regulations Relating to Transportation (Continued... REGULATIONS Inspection and Testing § 210.29 Operation standards (moving locomotives and rail cars). The operation standards for the noise emission levels of moving locomotives, rail cars, or consists of...

49 CFR 210.29 - Operation standards (moving locomotives and rail cars).

Code of Federal Regulations, 2013 CFR

2013-10-01

... cars). 210.29 Section 210.29 Transportation Other Regulations Relating to Transportation (Continued... REGULATIONS Inspection and Testing § 210.29 Operation standards (moving locomotives and rail cars). The operation standards for the noise emission levels of moving locomotives, rail cars, or consists of...

49 CFR 210.29 - Operation standards (moving locomotives and rail cars).

Code of Federal Regulations, 2012 CFR

2012-10-01

... cars). 210.29 Section 210.29 Transportation Other Regulations Relating to Transportation (Continued... REGULATIONS Inspection and Testing § 210.29 Operation standards (moving locomotives and rail cars). The operation standards for the noise emission levels of moving locomotives, rail cars, or consists of...

49 CFR 210.29 - Operation standards (moving locomotives and rail cars).

Code of Federal Regulations, 2014 CFR

2014-10-01

... cars). 210.29 Section 210.29 Transportation Other Regulations Relating to Transportation (Continued... REGULATIONS Inspection and Testing § 210.29 Operation standards (moving locomotives and rail cars). The operation standards for the noise emission levels of moving locomotives, rail cars, or consists of...

Standoff detection of trace amounts of solids by nonlinear Raman spectroscopy using shaped femtosecond pulses

NASA Astrophysics Data System (ADS)

Katz, O.; Natan, A.; Silberberg, Y.; Rosenwaks, S.

2008-04-01

We demonstrate a single-beam, standoff (>10m) detection and identification of various materials including minute amounts of explosives under ambient light conditions. This is obtained by multiplex coherent anti-Stokes Raman scattering spectroscopy (CARS) using a single femtosecond phase-shaped laser pulse. We exploit the strong nonresonant background for amplification of the backscattered resonant CARS signals by employing a homodyne detection scheme. The simple and highly sensitive spectroscopic technique has a potential for hazardous materials standoff detection applications.

Antibacterial and efflux pump inhibitors of thymol and carvacrol against food-borne pathogens.

PubMed

Miladi, Hanene; Zmantar, Tarek; Chaabouni, Yassine; Fedhila, Kais; Bakhrouf, Amina; Mahdouani, Kacem; Chaieb, Kamel

2016-10-01

In this study thymol (THY) and carvacrol (CAR), two monoterpenic phenol produced by various aromatic plants, was tested for their antibacterial and efflux pump inhibitors potencies against a panel of clinical and foodborne pathogenes. Our results demonstrated a substantial susceptibility of the tested bacteria toward THY and CAR. Especially, THY displayed a strong inhibitory activity (MIC's values ranged from 32 to 64 μg/mL) against the majority of the tested strains compared to CAR. Moreover, a significant reduction in MIC's of TET and benzalkonium chloride (QAC) were noticed when tested in combinations with THY and CAR. Their synergic effect was more significant in the case of THY which resulted a reduction of MIC's values of TET (2-8 fold) and QAC (2-8 fold). We noted also that THY and CAR inhibited the ethidium bromide (EtBr) cell efflux in a concentration-dependent manner. The rate of EtBr accumulation in food-borne pathogen was enhanced with THY and CAR (0, 250 and 500 μg/mL). The lowest concentration causing 50% of EtBr efflux inhibition (IC 50) was noticed in Salmonella enteritidis (1129) at 150 μg/mL of THY and 190 μg/mL of CAR respectively. These findings indicate that THY and CAR may serve as potential sources of efflux pump inhibitor in food-borne pathogens. Copyright © 2016 Elsevier Ltd. All rights reserved.

A single amino acid controls the functional switch of human constitutive androstane receptor (CAR) 1 to the xenobiotic-sensitive splicing variant CAR3.

PubMed

Chen, Tao; Tompkins, Leslie M; Li, Linhao; Li, Haishan; Kim, Gregory; Zheng, Yuxin; Wang, Hongbing

2010-01-01

The constitutive androstane receptor (CAR) is constitutively activated in immortalized cell lines independent of xenobiotic stimuli. This feature of CAR has limited its use as a sensor for xenobiotic-induced expression of drug-metabolizing enzymes. Recent reports, however, reveal that a splicing variant of human CAR (hCAR3), which contains an insertion of five amino acids (APYLT), exhibits low basal but xenobiotic-inducible activities in cell-based reporter assays. Nonetheless, the underlying mechanisms of this functional shift are not well understood. We have now generated chimeric constructs containing various residues of the five amino acids of hCAR3 and examined their response to typical hCAR activators. Our results showed that the retention of alanine (hCAR1+A) alone is sufficient to confer the constitutively activated hCAR1 to the xenobiotic-sensitive hCAR3. It is noteworthy that hCAR1+A was significantly activated by a series of known hCAR activators, and displayed activation superior to that of hCAR3. Moreover, intracellular localization assays revealed that hCAR1+A exhibits nuclear accumulation upon 6-(4-chlorophenyl) imidazo[2,1-b][1,3]thiazole-5-carbaldehyde-O-(3,4-dichlorobenzyl) oxime (CITCO) treatment in COS1 cells, which differs from the spontaneous nuclear distribution of hCAR1 and the nontranslocatable hCAR3. Mammalian two-hybrid and glutathione S-transferase pull-down assays further demonstrated that hCAR1+A interacts with the coactivator SRC-1 and GRIP-1 at low level before activation, while at significantly enhanced level in the presence of CITCO. Thus, the alanine residue in the insertion of hCAR3 seems in charge of the xenobiotic response of hCAR3 through direct and indirect mechanisms. Activation of hCAR1+A may represent a sensitive avenue for the identification of hCAR activators.

A Single Amino Acid Controls the Functional Switch of Human Constitutive Androstane Receptor (CAR) 1 to the Xenobiotic-Sensitive Splicing Variant CAR3

PubMed Central

Chen, Tao; Tompkins, Leslie M.; Li, Linhao; Li, Haishan; Kim, Gregory; Zheng, Yuxin

2010-01-01

The constitutive androstane receptor (CAR) is constitutively activated in immortalized cell lines independent of xenobiotic stimuli. This feature of CAR has limited its use as a sensor for xenobiotic-induced expression of drug-metabolizing enzymes. Recent reports, however, reveal that a splicing variant of human CAR (hCAR3), which contains an insertion of five amino acids (APYLT), exhibits low basal but xenobiotic-inducible activities in cell-based reporter assays. Nonetheless, the underlying mechanisms of this functional shift are not well understood. We have now generated chimeric constructs containing various residues of the five amino acids of hCAR3 and examined their response to typical hCAR activators. Our results showed that the retention of alanine (hCAR1+A) alone is sufficient to confer the constitutively activated hCAR1 to the xenobiotic-sensitive hCAR3. It is noteworthy that hCAR1+A was significantly activated by a series of known hCAR activators, and displayed activation superior to that of hCAR3. Moreover, intracellular localization assays revealed that hCAR1+A exhibits nuclear accumulation upon 6-(4-chlorophenyl) imidazo[2,1-b][1,3]thiazole-5-carbaldehyde-O-(3,4-dichlorobenzyl) oxime (CITCO) treatment in COS1 cells, which differs from the spontaneous nuclear distribution of hCAR1 and the nontranslocatable hCAR3. Mammalian two-hybrid and glutathione S-transferase pull-down assays further demonstrated that hCAR1+A interacts with the coactivator SRC-1 and GRIP-1 at low level before activation, while at significantly enhanced level in the presence of CITCO. Thus, the alanine residue in the insertion of hCAR3 seems in charge of the xenobiotic response of hCAR3 through direct and indirect mechanisms. Activation of hCAR1+A may represent a sensitive avenue for the identification of hCAR activators. PMID:19820207

Helical grip for the cable cars of San Francisco

NASA Technical Reports Server (NTRS)

Peyran, R. J.

1979-01-01

A helical cable car grip to minimize high maintenance costs of San Francisco's cable car operation is presented. The grip establishes a rolling contact between the cable and grip to reduce sliding friction and associated cable wear. The design, development, and testing of the helical cable car grip are described.

Noise level measurements on the UMTA Mark I Diagnostic Car (R42 MODEL)

DOT National Transportation Integrated Search

1971-10-01

The R42 Model mass transit car currently operating on the "N" line of the new York City Transit System was selected for experimentation and tests. For this purpose, the car was instrumented and designated as the UMTA Mark I Diagnostic Car. Noise leve...

"Off-Label" Drug Use

MedlinePlus

... Student Debt Special Report All Money More on Money Best & Worst Banks According to Consumer Reports Members ... ve tested. CARS Best New Car Deals Save money on the cars that Consumer Reports recommends. GENERATOR ...

Interpreting CARS images of tissue within the C-H-stretching region

NASA Astrophysics Data System (ADS)

Dietzek, Benjamin; Meyer, Tobias; Medyukhina, Anna; Bergner, Norbert; Krafft, Christoph; Romeike, Bernd F. M.; Reichart, Rupert; Kalff, Rolf; Schmitt, Michael; Popp, Jürgen

2014-03-01

Single band coherent anti-Stokes Raman scattering (CARS) microscopy within the CH-stretching region is applied to detect individual cells and nuclei of human brain tissue and brain tumors - an information which allows for histopathologic grading of the tissue. The CARS image contrast within the C-H-stretching region correlated to the tissue composition. Based on the specific application example of identifying nuclei within (coherent) Raman images of neurotissue sections, we shall derive general design parameters for lasers optimally suited to serve in a clinical environment and discuss the potential of recently developed methods to analyze spectrally resolved CARS images and image segmentation algorithms.

Engineering Tests for Energy Storage Cars at the Transportation Test Center : Volume 2. Performance, Power Consumption and Radio Frequency Interference Tests

DOT National Transportation Integrated Search

1977-05-01

The primary purpose of the tests documented herein was to demonstrate the principles and feasibility of an energy-storage-type propulsion system, and its adaptability to an existing car design. The test program comprised four phases of tests on two N...

36 CFR § 1192.111 - General.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true General. § 1192.111 Section § 1192.111 Parks, Forests, and Public Property ARCHITECTURAL AND TRANSPORTATION BARRIERS COMPLIANCE BOARD... rail passenger coaches and food service cars (other than single-level dining cars) shall comply with...

Proof Pressure Evaluation of Worn Passenger Car Tire Carcasses

DOT National Transportation Integrated Search

1976-11-01

Test work is described that examines the value of hydrostatic proof pressure testing in screening worn tire carcasses before retreading. Results are given from dynamometer wheel tests on a significant sample of retreaded passenger car tires. Each sam...

Results of Rolling Resistance Measurements of Twelve Radial Passenger Car Tires

DOT National Transportation Integrated Search

1980-01-01

The Calspan Tire Research Facility is described, including the test procedure, a tire test schedule, and a tire identification schedule for testing low-rolling resistance radial passenger car tires. The equilibrium rolling resistance was measured on ...

A dual chain chimeric antigen receptor (CAR) in the native antibody format for targeting immune cells towards cancer cells without the need of an scFv.

PubMed

Faitschuk, E; Nagy, V; Hombach, A A; Abken, H

2016-10-01

Adoptive cell therapy with chimeric antigen receptor (CAR)-modified T cells showed remarkable therapeutic efficacy in the treatment of leukaemia/lymphoma. However, the application to a variety of cancer entities is often constricted by the non-availability of a single chain antibody (scFv), which is usually the targeting domain in a CAR, while antibodies in the natural format are often available. To overcome the limitation, we designed a CAR that uses an antibody in its natural configuration for binding. Such CAR consists of two chains, the immunoglobulin light and heavy chain with their constant regions, whereby the heavy chain is anchored to the membrane and linked to an intracellular signalling domain for T-cell activation. The two chains form a stable heterodimer, a so-called dual chain CAR (dcCAR), and bind with high affinity and in a specific manner to their cognate antigen. By specific binding, the dcCAR activates engineered T cells for the release of pro-inflammatory cytokines and for target cell lysis. We provide evidence by three examples that the dcCAR format is universally applicable and thereby broadens the CAR cell therapy towards a larger variety of targets for which an scFv antibody is not available.

Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes

PubMed Central

Di Napoli, Claudia; Pope, Iestyn; Masia, Francesco; Watson, Peter; Langbein, Wolfgang; Borri, Paola

2014-01-01

In this work, we demonstrate the applicability of coherent anti-Stokes Raman scattering (CARS) micro-spectroscopy for quantitative chemical imaging of saturated and unsaturated lipids in human stem-cell derived adipocytes. We compare dual-frequency/differential CARS (D-CARS), which enables rapid imaging and simple data analysis, with broadband hyperspectral CARS microscopy analyzed using an unsupervised phase-retrieval and factorization method recently developed by us for quantitative chemical image analysis. Measurements were taken in the vibrational fingerprint region (1200–2000/cm) and in the CH stretch region (2600–3300/cm) using a home-built CARS set-up which enables hyperspectral imaging with 10/cm resolution via spectral focussing from a single broadband 5 fs Ti:Sa laser source. Through a ratiometric analysis, both D-CARS and phase-retrieved hyperspectral CARS determine the concentration of unsaturated lipids with comparable accuracy in the fingerprint region, while in the CH stretch region D-CARS provides only a qualitative contrast owing to its non-linear behavior. When analyzing hyperspectral CARS images using the blind factorization into susceptibilities and concentrations of chemical components recently demonstrated by us, we are able to determine vol:vol concentrations of different lipid components and spatially resolve inhomogeneities in lipid composition with superior accuracy compared to state-of-the art ratiometric methods. PMID:24877002

Analysis of car’s frontal collision against pole

NASA Astrophysics Data System (ADS)

Ispas, N.; Nastasoiu, M.

2017-10-01

Reducing the effects of traffic accidents over the occupants is a major objective of collision attempts. Impacts between the car and the pole are very dangerous for the physical integrity of the car’s occupants. To minimalize the effects of such events on the passengers of a vehicle, a whole series of efforts by both designers and experienced engineers led to increasingly the vehicles safety. The main aim of these paper is to quantify the influences over the car passengers of loads involved by car against pole collisions using the same car model at different speeds. Also, this kind on occupant influences were study using a small car model. Other goal of the paper was the study of the cars stiffness in frontal collision against the pole. The paper’s experimental results were obtained by support of DSD, Dr. Steffan Datentechnik GmbH - Linz, Austria. The described tests were performed in full test facility of DSD Linz, in “Easter 2016 PC-Crash Seminar”. Cars accelerations, velocities, rotations angles after pole impact were registered and discussed. The novelty of the paper consists in studies referred for the same car model involved in car against pole collisions at different impact speeds. Paper’s conclusions can be future used for car safety improvement.

49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

Code of Federal Regulations, 2012 CFR

2012-10-01

... tank car owner can determine by analysis (e.g., finite element analysis, damage-tolerance analysis, or... missing or loose bolts, nuts, or elements that may make the tank car unsafe for transportation; (5) An... must ensure the structural elements on the tank car qualify with the applicable requirements of this...

49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

Code of Federal Regulations, 2013 CFR

2013-10-01

... tank car owner can determine by analysis (e.g., finite element analysis, damage-tolerance analysis, or... missing or loose bolts, nuts, or elements that may make the tank car unsafe for transportation; (5) An... must ensure the structural elements on the tank car qualify with the applicable requirements of this...

49 CFR 180.509 - Requirements for inspection and test of specification tank cars.

Code of Federal Regulations, 2014 CFR

2014-10-01

... tank car owner can determine by analysis (e.g., finite element analysis, damage-tolerance analysis, or... missing or loose bolts, nuts, or elements that may make the tank car unsafe for transportation; (5) An... must ensure the structural elements on the tank car qualify with the applicable requirements of this...

Passenger rail two-car impact test. Volume 2 : summary of occupant protection program

DOT National Transportation Integrated Search

2002-01-01

Two full-scale impact tests of rail cars fitted with seat/occupant experiments were conducted at the Federal Railroad Administrations Transportation Technology Center located in Pueblo, Colorado. The first test was conducted on November 16, 1999, ...

Validation of the American version of the CareGiver Oncology Quality of Life (CarGOQoL) questionnaire.

PubMed

Kaveney, Sarah C; Baumstarck, Karine; Minaya-Flores, Patricia; Shannon, Tarrah; Symes, Philip; Loundou, Anderson; Auquier, Pascal

2016-05-28

The CareGiver Oncology Quality of Life (CarGOQoL) questionnaire, a 29-item, multidimensional, self-administered questionnaire, was validated using a large French sample. We reported the linguistic validation process and the metric validity of the English version of CarGOQoL in the United- States. The translation process consisted of 3 consecutive steps: forward-backward translation, acceptability testing, and cognitive interviews. The psychometric testing was applied to caregivers of consecutive patients with representative cancers who were recruited from the Regional Cancer Center in northwestern Pennsylvania. All individuals completed the CarGOQoL at baseline, day- 30, and day- 90. Internal consistency, reliability, external validity, reproducibility, and sensitivity to change were tested. The translated version was validated on a total of 87 American cancer caregivers. The dimensions of the CarGOQoL generally demonstrated a high internal consistency (Cronbach's alpha > 0.70 for all but four domain scores). External validity testing revealed that the CarGOQoL index score correlated significantly with all SF-36 dimension scores except the physical composite score (Pearson's correlation: 0.28-0.70). Reproducibility was satisfactory at day- 30 (intraclass correlation coefficient: 0.46-0.94) and day- 90 (0.43-0.92). Four specific dimensions of CarGOQoL showed responsiveness: the Psychological well-being, the Relationships with health care system, the Social support and the Finances. The American version of the CarGOQoL constitutes a useful instrument to measure QoL in caregivers of cancer patients in the United- States.

Intraperitoneal immunotherapy with T cells stably and transiently expressing anti-EpCAM CAR in xenograft models of peritoneal carcinomatosis

PubMed Central

Chi, Zhixia; Du, Shou-Hui; Chen, Can; Tay, Johan C.K.; Toh, Han Chong; Connolly, John E.; Xu, Xue Hu; Wang, Shu

2017-01-01

The epithelial cell adhesion molecule (EpCAM) is overexpressed in a wide variety of tumor types, including peritoneal carcinomatosis (PC) from gastrointestinal and gynecological malignancies. To develop a chimeric antigen receptor T (CART) cell therapy approach to treat patients with end-stage PC, we constructed third generation CARs specific to EpCAM using the 4D5MOC-B single chain variable fragment. CART cells were generated with lentiviral transduction and exhibited specific in vitro killing activity against EpCAM-positive human ovarian and colorectal cancer cells. A single intraperitoneal injection of the CART cells eradicated established ovarian xenografts and resulted in significantly prolonged animal survival. Since EpCAM is also expressed on normal epithelium, anti-EpCAM CART cells were generated by mRNA electroporation that display a controlled cytolytic activity with a limited CAR expression duration. Multiple repeated infusions of these RNA CAR-modified T cells delayed disease progression in immunodeficient mice bearing well-established peritoneal ovarian and colorectal xenografts. Thus, our study demonstrates the effectiveness of using anti-EpCAM CAR-expressing T cells for local treatment of PC in mice. The possibility of using this approach for clinical treatment of EpCAM-positive gastrointestinal and gynecological malignancies warrants further validation. PMID:28088790

Optical Automatic Car Identification (OACI) Field Test Program

DOT National Transportation Integrated Search

1976-05-01

The results of the Optical Automatic Car Identification (OACI) tests at Chicago conducted from August 16 to September 4, 1975 are presented. The main purpose of this test was to determine the suitability of optics as a principle of operation for an a...

Have pedestrian subsystem tests improved passenger car front shape?

PubMed

Li, Guibing; Wang, Fang; Otte, Dietmar; Cai, Zhihua; Simms, Ciaran

2018-06-01

Subsystem impactor tests are the main approaches for evaluation of safety performance of vehicle front design for pedestrian protection in legislative regulations. However, the main aspects of vehicle safety for pedestrians are shape and stiffness, and though it is clear that subsystem impact tests encourage lower vehicle front stiffness, it is unclear whether they promote improved vehicle front shapes for pedestrian protection. The purpose of this paper is therefore to investigate the effects of European pedestrian safety regulations on passenger car front shape and pedestrian injury risk using recent German In-Depth Accident Study (GIDAS) pedestrian collision data and numerical simulations. Firstly, a sample of 579 pedestrian collision cases involving 190 different car models between 2000-2015 extracted from the GIDAS was used to compare front-end shapes of passenger cars manufactured before and after the legislative pedestrian safety regulations were introduced in Europe. The focus was on changes in passenger car front shape and differences in pedestrian AIS2+ (Abbreviated Injury Scale at least level 2) leg, pelvis/femur and head injury risk observed in collisions. Multi-body simulations were also used to assess changes in vehicle aggressivity due to the observed changes in vehicle shape. The results show that newer passenger cars tend to have a flatter and wider bumper, higher bonnet leading edge, shorter and steeper bonnet and a shallower windscreen. Both the collision data and the numerical simulations indicate that newer passenger car front bumper designs are significantly safer for pedestrians' legs. However, the results also show that the higher bonnet leading edge in newer passenger cars is poor for pedestrian pelvis/femur protection, even though newer cars show an obviously lower AIS2+ injury risk to younger pedestrians in collisions. Newer cars have a lower AIS2+ head injury risk for pedestrians in collisions, but the numerical analysis indicate that this is not likely due to shape changes in passenger car fronts. Overall, the introduction of pedestrian safety regulations has resulted in reductions in pedestrian injury risk, but further benefits would accrue from tests which promote a lower bonnet leading edge. The influence of vehicle shape on pedestrian head injury risk remains unclear. Copyright © 2018 Elsevier Ltd. All rights reserved.

Temperature measurements in metalized propellant combustion using hybrid fs/ps coherent anti-Stokes Raman scattering.

PubMed

Kearney, Sean P; Guildenbecher, Daniel R

2016-06-20

We apply ultrafast pure-rotational coherent anti-Stokes Raman scattering (CARS) for temperature and relative oxygen concentration measurements in the plume emanating from a burning, aluminized ammonium-perchlorate propellant strand. Combustion of these metal-based propellants is a particularly hostile environment for laser-based diagnostics, with intense background luminosity and scattering from hot metal particles as large as several hundred micrometers in diameter. CARS spectra that were previously obtained using nanosecond pulsed lasers in an aluminum-particle-seeded flame are examined and are determined to be severely impacted by nonresonant background, presumably as a result of the plasma formed by particulate-enhanced laser-induced breakdown. Introduction of femtosecond/picosecond (fs/ps) laser pulses improves CARS detection by providing time-gated elimination of strong nonresonant background interference. Single-laser-shot fs/ps CARS spectra were acquired from the burning propellant plume, with picosecond probe-pulse delays of 0 and 16 ps from the femtosecond pump and Stokes pulses. At zero delay, nonresonant background overwhelms the Raman-resonant spectroscopic features. Time-delayed probing results in the acquisition of background-free spectra that were successfully fit for temperature and relative oxygen content. Temperature probability densities and temperature/oxygen correlations were constructed from ensembles of several thousand single-laser-shot measurements with the CARS measurement volume positioned within 3 mm or less of the burning propellant surface. The results show that ultrafast CARS is a potentially enabling technology for probing harsh, particle-laden flame environments.

Temperature measurements in metalized propellant combustion using hybrid fs/ps coherent anti-Stokes Raman scattering

DOE PAGES

Kearney, Sean P.; Guildenbecher, Daniel R.

2016-06-20

We apply ultrafast pure-rotational coherent anti-Stokes Raman scattering (CARS) for temperature and relative oxygen concentration measurements in the plume emanating from a burning, aluminized ammonium-perchlorate propellant strand. Combustion of these metal-based propellants is a particularly hostile environment for laser-based diagnostics, with intense background luminosity and scattering from hot metal particles as large as several hundred micrometers in diameter. CARS spectra that were previously obtained using nanosecond pulsed lasers in an aluminum-particle-seeded flame are examined and are determined to be severely impacted by nonresonant background, presumably as a result of the plasma formed by particulate-enhanced laser-induced breakdown. Introduction of femtosecond/picosecond (fs/ps)more » laser pulses improves CARS detection by providing time-gated elimination of strong nonresonant background interference. Single-laser-shot fs/ps CARS spectra were acquired from the burning propellant plume, with picosecond probe-pulse delays of 0 and 16 ps from the femtosecond pump and Stokes pulses. At zero delay, nonresonant background overwhelms the Raman-resonant spectroscopic features. Time-delayed probing results in the acquisition of background-free spectra that were successfully fit for temperature and relative oxygen content. Temperature probability densities and temperature/oxygen correlations were constructed from ensembles of several thousand single-laser-shot measurements with the CARS measurement volume positioned within 3 mm or less of the burning propellant surface. Lastly, the results show that ultrafast CARS is a potentially enabling technology for probing harsh, particle-laden flame environments.« less

Temperature measurements in metalized propellant combustion using hybrid fs/ps coherent anti-Stokes Raman scattering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kearney, Sean P.; Guildenbecher, Daniel R.

We apply ultrafast pure-rotational coherent anti-Stokes Raman scattering (CARS) for temperature and relative oxygen concentration measurements in the plume emanating from a burning, aluminized ammonium-perchlorate propellant strand. Combustion of these metal-based propellants is a particularly hostile environment for laser-based diagnostics, with intense background luminosity and scattering from hot metal particles as large as several hundred micrometers in diameter. CARS spectra that were previously obtained using nanosecond pulsed lasers in an aluminum-particle-seeded flame are examined and are determined to be severely impacted by nonresonant background, presumably as a result of the plasma formed by particulate-enhanced laser-induced breakdown. Introduction of femtosecond/picosecond (fs/ps)more » laser pulses improves CARS detection by providing time-gated elimination of strong nonresonant background interference. Single-laser-shot fs/ps CARS spectra were acquired from the burning propellant plume, with picosecond probe-pulse delays of 0 and 16 ps from the femtosecond pump and Stokes pulses. At zero delay, nonresonant background overwhelms the Raman-resonant spectroscopic features. Time-delayed probing results in the acquisition of background-free spectra that were successfully fit for temperature and relative oxygen content. Temperature probability densities and temperature/oxygen correlations were constructed from ensembles of several thousand single-laser-shot measurements with the CARS measurement volume positioned within 3 mm or less of the burning propellant surface. Lastly, the results show that ultrafast CARS is a potentially enabling technology for probing harsh, particle-laden flame environments.« less

Dual fuel injection piggyback controller system

NASA Astrophysics Data System (ADS)

Muji, Siti Zarina Mohd.; Hassanal, Muhammad Amirul Hafeez; Lee, Chua King; Fawzi, Mas; Zulkifli, Fathul Hakim

2017-09-01

Dual-fuel injection is an effort to reduce the dependency on diesel and gasoline fuel. Generally, there are two approaches to implement the dual-fuel injection in car system. The first approach is changing the whole injector of the car engine, the consequence is excessive high cost. Alternatively, it also can be achieved by manipulating the system's control signal especially the Electronic Control Unit (ECU) signal. Hence, the study focuses to develop a dual injection timing controller system that likely adopted to control injection time and quantity of compressed natural gas (CNG) and diesel fuel. In this system, Raspberry Pi 3 reacts as main controller unit to receive ECU signal, analyze it and then manipulate its duty cycle to be fed into the Electronic Driver Unit (EDU). The manipulation has changed the duty cycle to two pulses instead of single pulse. A particular pulse mainly used to control injection of diesel fuel and another pulse controls injection of Compressed Natural Gas (CNG). The test indicated promising results that the system can be implemented in the car as piggyback system. This article, which was originally published online on 14 September 2017, contained an error in the acknowledgment section. The corrected acknowledgment appears in the Corrigendum attached to the pdf.

Spatially and Temporally Resolved Measurements of Velocity in a H2-air Combustion-Heated Supersonic Jet

NASA Technical Reports Server (NTRS)

Bivolaru, Daniel; Cutler, Andrew D.; Danehy, Paul M.; Gaffney, Richard L.; Baurle, Robert a.

2009-01-01

This paper presents simultaneous measurements at multiple points of two orthogonal components of flow velocity using a single-shot interferometric Rayleigh scattering (IRS) technique. The measurements are performed on a large-scale Mach 1.6 (Mach 5.5 enthalpy) H2-air combustion jet during the 2007 test campaign in the Direct Connect Supersonic Combustion Test facility at NASA Langley Research Center. The measurements are performed simultaneously with CARS (Coherent Anti-stokes Raman Spectroscopy) using a combined CARS-IRS instrument with a common path 9-nanosecond pulsed, injection-seeded, 532-nm Nd:YAG laser probe pulse. The paper summarizes the measurements of velocities along the core of the vitiated air flow as well as two radial profiles. The average velocity measurement near the centerline at the closest point from the nozzle exit compares favorably with the CFD calculations using the VULCAN code. Further downstream, the measured axial velocity shows overall higher values than predicted with a trend of convergence at further distances. Larger discrepancies are shown in the radial profiles.

Secretary Perry Drives a Hydrogen Fuel Cell Car (U.S. Department of Energy)

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

Secretary Rick Perry test drives a fuel cell car. The car can travel more than 300 miles on just 5 kilograms of hydrogen. Government and industry research reduced the cost of fuel cells for transportation by 60% since 2006. That’s helped put more than 2,500 fuel cell cars on the road.

Pressure Fluctuation Characteristics of Narrow Gauge Train Running Through Tunnel

NASA Astrophysics Data System (ADS)

Suzuki, Masahiro; Sakuma, Yutaka

Pressure fluctuations on the sides of narrow (1067 mm) gauge trains running in tunnels are measured for the first time to investigate the aerodynamic force acting on the trains. The present measurements are compared with earlier measurements obtained with the Shinkansen trains. The results are as follows: (1) The aerodynamic force, which stems from pressure fluctuations on the sides of cars, puts the energy into the vibration of the car body running through a tunnel. (2) While the pressure fluctuations appear only on one of the two sides of the trains running in double-track tunnels, the fluctuations in opposite phase on both sides in single-track tunnels. (3) The on-track test data of the narrow gauge trains show the same tendency as those of the Shinkansen trains, although it is suggested that the pressure fluctuations develop faster along the narrow gauge trains than the Shinkansen trains.

US Steel Gary Works land based pushing emissions control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Randolph, R.A.; Price, C.A.

1983-01-01

To meet air quality standards at its Gary Works Coke Plant in Gary, Indiana, US Steel Corporation has installed pushing emission control systems for its five (77) oven, three meter coke batteries. The pushing emission control system consists of a hooded coke guide, single spot catch car, stationary emission capture ducts and remote gas cleaning baghouse with precoat capabilities. The system is providing effective emission control. In addition, there are corollary benefits. The operation of the single spot catch cars is easier and safer and coke moisture variables have been reduced.

Multiplex CARS spectroscopy of Rochelle salt crystal

NASA Astrophysics Data System (ADS)

Hadjichristov, G. B.; Kircheva, P. P.; Kirov, N.

1996-08-01

An optical four-wave mixing (FWM) described by the nonlinear susceptibility x(3) ( ωas = 2 ωp - ωs) is studied in a single crystal of Rochelle salt (NaKC 4H 4O 6·H 2O). In addition to the coherent anti-Stokes Raman Scattering (CARS) structure, the FWM spectra consist of nonvibrational (background) components. After analysis of the FWM spectra in the range from 1100 to 1450 cm -1, the nonresonant (electronic) susceptibility elements xelzzzz and xelxxxx of Rochelle salt single crystal are estimated.

Assessment of driving after stroke--a pluridisciplinary task.

PubMed

Ponsford, A-S; Viitanen, M; Lundberg, C; Johansson, K

2008-03-01

The aim of the study was to analyze the assessment procedure and identify predictors for the team decision when assessing fitness to drive a car after stroke. The material used was a retrospective data set with 200 stroke clients from Queen Elisabeth's Foundation Mobility Centre at Banstead UK. Fifty-four percent of clients were considered fit to continue driving where 9% could resume driving after car adaptation and training. Important factors for the outcome were vision (acuity and field), neuropsychological functions (divided attention), and track and/or on road test (reaction time, anticipation, speed, and positioning). Cognitive impairment was the main problem in those who failed the driving test and judged not fit for continued driving. Car adaptation, mainly comprising infrared transmitted secondary controls together with automatic transmission was recommended in 35% of the cases. The contribution of different specialist groups appears to be necessary for an effective evaluation, but the assessment procedure can be done more cost-effectively by dividing it into two separate parts and removing certain subtests. The in-car track test is an important part of the assessment procedure with a high face validity and could in many cases make it unnecessary to perform in-traffic tests with unsafe drivers. Car adaptation is often necessary for a client with pronounced hemi-paresis and a full road test can for those only be performed after training the use of car controls.

Railroad tank car nondestructive methods evaluation.

DOT National Transportation Integrated Search

2002-01-01

An evaluation of nondestructive testing (NDT) methods, authorized for use in replacing the current hydrostatic pressure test for qualification or re-qualification of railroad tank cars, has been performed by the Transportation Technology Center, Inc....

Simulation analysis of the EUSAMA Plus suspension testing method including the impact of the vehicle untested side

NASA Astrophysics Data System (ADS)

Dobaj, K.

2016-09-01

The work deals with the simulation analysis of the half car vehicle model parameters on the suspension testing results. The Matlab simulation software was used. The considered model parameters are involved with the shock absorber damping coefficient, the tire radial stiffness, the car width and the rocker arm length. The consistent vibrations of both test plates were considered. Both wheels of the car were subjected to identical vibration, with frequency changed similar to the EUSAMA Plus principle. The shock absorber damping coefficient (for several values of the car width and rocker arm length) was changed on one and both sides of the vehicle. The obtained results are essential for the new suspension testing algorithm (basing on the EUSAMA Plus principle), which will be the aim of the further author's work.

76 FR 55829 - Federal Motor Vehicle Safety Standards; Electronic Stability Control Systems

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-09

.... Benefits of ESC Electronic stability control (ESC) systems use automatic computer- controlled braking of... demonstrated that these systems reduce fatal single-vehicle crashes of passenger cars by 55 percent and fatal... potential to prevent 56 percent of the fatal passenger car rollovers and 74 percent of the fatal LTV first...

Simulation System of Car Crash Test in C-NCAP Analysis Based on an Improved Apriori Algorithm*

NASA Astrophysics Data System (ADS)

Xiang, LI

In order to analysis car crash test in C-NCAP, an improved algorithm is given based on Apriori algorithm in this paper. The new algorithm is implemented with vertical data layout, breadth first searching, and intersecting. It takes advantage of the efficiency of vertical data layout and intersecting, and prunes candidate frequent item sets like Apriori. Finally, the new algorithm is applied in simulation of car crash test analysis system. The result shows that the relations will affect the C-NCAP test results, and it can provide a reference for the automotive design.

Development of conventional passenger cab car end structure designs for full scale testing

DOT National Transportation Integrated Search

2006-12-01

The Volpe Center is supporting the Federal Railroad Administration's full-scale testing program to understand and improve rail vehicle cashworthiness. The objectie of one of the sets of tests in this program is determining the behavior of cab car end...

Effects of smoke-free laws on alcohol-related car crashes in California and New York: time series analyses from 1982 to 2008.

PubMed

Bernat, Debra H; Maldonado-Molina, Mildred; Hyland, Andrew; Wagenaar, Alexander C

2013-02-01

We examined effects of New York and California's statewide smoke-free restaurant and bar policies on alcohol-related car crash fatalities. We used an interrupted time-series design from 1982 to 2008, with 312 monthly observations, to examine the effect of each state's law on single-vehicle-nighttime crashes and crashes involving a driver with a blood alcohol concentration of 0.08 grams per deciliter or greater. Implementation of New York and California's statewide smoke-free policies was not associated with alcohol-related car crash fatalities. Additionally, analyses showed no effect of New York's smoke-free policy on alcohol-related car crash fatalities in communities along the Pennsylvania-New York border. Statewide smoke-free restaurant and bar laws do not appear to affect rates of alcohol-related car crashes.

Impact of smoking on in-vehicle fine particle exposure during driving

NASA Astrophysics Data System (ADS)

Sohn, Hongji; Lee, Kiyoung

2010-09-01

Indoor smoking ban in public places can reduce secondhand smoke (SHS) exposure. However, smoking in cars and homes has continued. The purpose of this study was to assess particulate matter less than 2.5 μm (PM 2.5) concentration in moving cars with different window opening conditions. The PM 2.5 level was measured by an aerosol spectrometer inside and outside moving cars simultaneously, along with ultrafine particle (UFP) number concentration, speed, temperature and humidity inside cars. Two sport utility vehicles were used. Three different ventilation conditions were evaluated by up to 20 repeated experiments. In the pre-smoking phase, average in-vehicle PM 2.5 concentrations were 16-17 μg m -3. Regardless of different window opening conditions, the PM 2.5 levels promptly increased when smoking occurred and decreased after cigarette was extinguished. Although only a single cigarette was smoked, the average PM 2.5 levels were 506-1307 μg m -3 with different window opening conditions. When smoking was ceased, the average PM 2.5 levels for 15 min were several times higher than the US National Ambient Air Quality Standard of 35 μg m -3. It took longer than 10 min to reach the level of the pre-smoking phase. Although UFP levels had a similar temporal profile of PM 2.5, the increased levels during the smoking phase were relatively small. This study demonstrated that the SHS exposure in cars with just a single cigarette being smoked could exceed the US EPA NAAQS under realistic window opening conditions. Therefore, the findings support the need for public education against smoking in cars and advocacy for a smoke-free car policy.

Preclinical Models in Chimeric Antigen Receptor-Engineered T-Cell Therapy.

PubMed

Siegler, Elizabeth Louise; Wang, Pin

2018-05-01

Cancer immunotherapy has enormous potential in inducing long-term remission in cancer patients, and chimeric antigen receptor (CAR)-engineered T cells have been largely successful in treating hematological malignancies in the clinic. CAR-T therapy has not been as effective in treating solid tumors, in part due to the immunosuppressive tumor microenvironment. Additionally, CAR-T therapy can cause dangerous side effects, including off-tumor toxicity, cytokine release syndrome, and neurotoxicity. Animal models of CAR-T therapy often fail to predict such adverse events and frequently overestimate the efficacy of the treatment. Nearly all preclinical CAR-T studies have been performed in mice, including syngeneic, xenograft, transgenic, and humanized mouse models. Recently, a few studies have used primate models to mimic clinical side effects better. To date, no single model perfectly recapitulates the human immune system and tumor microenvironment, and some models have revealed CAR-T limitations that were contradicted or missed entirely in other models. Careful model selection based on the primary goals of the study is a crucial step in evaluating CAR-T treatment. Advancements are being made in preclinical models, with the ultimate objective of providing safer, more effective CAR-T therapy to patients.

49 CFR 179.200-23 - Tests of pressure relief valves.

Code of Federal Regulations, 2013 CFR

2013-10-01

... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.200-23 Tests of...

49 CFR 179.200-23 - Tests of pressure relief valves.

Code of Federal Regulations, 2012 CFR

2012-10-01

... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.200-23 Tests of...

49 CFR 179.200-23 - Tests of pressure relief valves.

Code of Federal Regulations, 2014 CFR

2014-10-01

... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.200-23 Tests of...

KSC-2012-2888

NASA Image and Video Library

2012-05-21

CAPE CANAVERAL, Fla. – At the NASA Railroad yard at Kennedy Space Center in Florida, helium tank cars are lifted from their trucks onto flat cars in preparation for a journey to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s tank cars will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2890

NASA Image and Video Library

2012-05-21

CAPE CANAVERAL, Fla. – At the NASA Railroad yard at Kennedy Space Center in Florida, helium tank cars have been removed from their trucks and loaded onto flat cars in preparation for a journey to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s tank cars will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

The behaviour of a vehicle’s suspension system on dynamic testing conditions

NASA Astrophysics Data System (ADS)

Mihon, L.; Lontiş, N.; Deac, S.

2018-01-01

The paper presents a car suspension’s behaviour on dynamic testing conditions through theoretical and mathematical simulation on specific model, on the single traction wheel, according to the real vehicle and by experiment on the test bench by reproducing the road’s geometry and vehicle’s speed and measuring the acceleration and damping response of the suspension system on that wheel. There are taking in consideration also the geometry and properties of the tyre-wheel model and physical wheel’s properties. The results are important due to the suspension’s model properties which allows to extend the theory and applications to the whole vehicle for improving the vehicle’s dynamics.

KSC-2012-3055

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train crosses the railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The helium tank cars are positioned in the front and rear of the train. The long, thin tank car in the middle was used for liquid hydrogen, followed by a much larger tank car used for liquid oxygen. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3054

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train crosses the railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The helium tank cars are positioned in the front and rear of the train. The long, thin tank car in the middle was used for liquid hydrogen, followed by a much larger tank car used for liquid oxygen. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Small passenger car transmission test-Chevrolet 200 transmission

NASA Technical Reports Server (NTRS)

Bujold, M. P.

1980-01-01

The small passenger car transmission was tested to supply electric vehicle manufacturers with technical information regarding the performance of commerically available transmissions which would enable them to design a more energy efficient vehicle. With this information the manufacturers could estimate vehicle driving range as well as speed and torque requirements for specific road load performance characteristics. A 1979 Chevrolet Model 200 automatic transmission was tested per a passenger car automatic transmission test code (SAE J651b) which required drive performance, coast performance, and no load test conditions. The transmission attained maximum efficiencies in the mid-eighty percent range for both drive performance tests and coast performance tests. Torque, speed and efficiency curves map the complete performance characteristics for Chevrolet Model 200 transmission.

Cortisol in the morning and dimensions of anxiety, depression, and aggression in children from a general population and clinic-referred cohort: An integrated analysis. The TRAILS study.

PubMed

Dietrich, Andrea; Ormel, Johan; Buitelaar, Jan K; Verhulst, Frank C; Hoekstra, Pieter J; Hartman, Catharina A

2013-08-01

Anxiety and depressive problems have often been related to higher hypothalamic-pituitary-adrenal (HPA)-axis activity (basal morning cortisol levels and cortisol awakening response [CAR]) and externalizing problems to lower HPA-axis activity. However, associations appear weaker and more inconsistent than initially assumed. Previous studies from the Tracking Adolescents Individual Lives Study (TRAILS) suggested sex-differences in these relationships and differential associations with specific dimensions of depressive problems in a general population sample of children (10-12 years). Using the TRAILS population sample (n=1604), we tested hypotheses on the association between single day cortisol (basal morning levels and CAR) and specifically constructed dimensions of anxiety (cognitive versus somatic), depressive (cognitive-affective versus somatic), and externalizing problems (reactive versus proactive aggression), and explored the modifying role of sex. Moreover, we repeated analyses in an independent same-aged clinic-referred sample (n=357). Structural Equation Modeling was used to investigate the association between cortisol and higher- and lower-order (thus, broad and specific) problem dimensions based on self-reports in an integrated model. Overall, findings were consistent across the population and clinic-referred samples, as well as with the existing literature. Most support was found for higher cortisol (mainly CAR) in relation to depressive problems. However, in general, associations were weak in both samples. Therefore, the present results shed doubt on the relevance of single day cortisol measurements for problem behaviors in the milder range. Associations may be stronger in more severe or persistent psychopathology. Copyright © 2012 Elsevier Ltd. All rights reserved.

Application of Modern Design of Experiments to CARS Thermometry in a Model Scramjet Engine

NASA Technical Reports Server (NTRS)

Danehy, P. M.; DeLoach, R.; Cutler, A. D.

2002-01-01

We have applied formal experiment design and analysis to optimize the measurement of temperature in a supersonic combustor at NASA Langley Research Center. We used the coherent anti-Stokes Raman spectroscopy (CARS) technique to map the temperature distribution in the flowfield downstream of an 1160 K, Mach 2 freestream into which supersonic hydrogen fuel is injected at an angle of 30 degrees. CARS thermometry is inherently a single-point measurement technique; it was used to map thc flow by translating the measurement volume through the flowfield. The method known as "Modern Design of Experiments" (MDOE) was used to estimate the data volume required, design the test matrix, perform the experiment and analyze the resulting data. MDOE allowed us to match the volume of data acquired to the precision requirements of the customer. Furthermore, one aspect of MDOE, known as response surface methodology, allowed us to develop precise maps of the flowfield temperature, allowing interpolation between measurement points. An analytic function in two spatial variables was fit to the data from a single measurement plane. Fitting with a Cosine Series Bivariate Function allowed the mean temperature to be mapped with 95% confidence interval half-widths of +/- 30 Kelvin, comfortably meeting the confidence of +/- 50 Kelvin specified prior to performing the experiments. We estimate that applying MDOE to the present experiment saved a factor of 5 in data volume acquired, compared to experiments executed in the traditional manner. Furthermore, the precision requirements could have been met with less than half the data acquired.

Analysis of full-scale tank car shell impact tests

DOT National Transportation Integrated Search

2007-09-11

This paper describes analyses of a railroad tank car : impacted at its side by a ram car with a rigid punch. This : generalized collision, referred to as a shell impact, is examined : using nonlinear finite element analysis (FEA) and threedimensional...

Railroad Car Coupling Shock, Vertical Motion, and Roller Bearing Temperature

DOT National Transportation Integrated Search

1981-01-01

Data were collected in a study of railroad car operating environment. Measurements were made on wheel bearing operating temperatures, coupling impact shock, and vertical motion of the car due to rail travel. Tests were conducted using an instrumented...

Identification of chemical modulators of the constitutive activated receptor (CAR) in a gene expression compendium

PubMed Central

Oshida, Keiyu; Vasani, Naresh; Jones, Carlton; Moore, Tanya; Hester, Susan; Nesnow, Stephen; Auerbach, Scott; Geter, David R.; Aleksunes, Lauren M.; Thomas, Russell S.; Applegate, Dawn; Klaassen, Curtis D.; Corton, J. Christopher

2015-01-01

The nuclear receptor family member constitutive activated receptor (CAR) is activated by structurally diverse drugs and environmentally-relevant chemicals leading to transcriptional regulation of genes involved in xenobiotic metabolism and transport. Chronic activation of CAR increases liver cancer incidence in rodents, whereas suppression of CAR can lead to steatosis and insulin insensitivity. Here, analytical methods were developed to screen for chemical treatments in a gene expression compendium that lead to alteration of CAR activity. A gene expression biomarker signature of 83 CAR-dependent genes was identified using microarray profiles from the livers of wild-type and CAR-null mice after exposure to three structurally-diverse CAR activators (CITCO, phenobarbital, TCPOBOP). A rank-based algorithm (Running Fisher’s algorithm (p-value ≤ 10-4)) was used to evaluate the similarity between the CAR biomarker signature and a test set of 28 and 32 comparisons positive or negative, respectively, for CAR activation; the test resulted in a balanced accuracy of 97%. The biomarker signature was used to identify chemicals that activate or suppress CAR in an annotated mouse liver/primary hepatocyte gene expression database of ~1850 comparisons. CAR was activated by 1) activators of the aryl hydrocarbon receptor (AhR) in wild-type but not AhR-null mice, 2) pregnane X receptor (PXR) activators in wild-type and to lesser extents in PXR-null mice, and 3) activators of PPARα in wild-type and PPARα-null mice. CAR was consistently activated by five conazole fungicides and four perfluorinated compounds. Comparison of effects in wild-type and CAR-null mice showed that the fungicide propiconazole increased liver weight and hepatocyte proliferation in a CAR-dependent manner, whereas the perfluorinated compound perfluorooctanoic acid (PFOA) increased these endpoints in a CAR-independent manner. A number of compounds suppressed CAR coincident with increases in markers of inflammation including acetaminophen, concanavalin A, lipopolysaccharide, and 300 nm silica particles. In conclusion, we have shown that a CAR biomarker signature coupled with a rank-based similarity method accurately predicts CAR activation. This analytical approach, when applied to a gene expression compendium, increased the universe of known chemicals that directly or indirectly activate CAR, highlighting the promiscuous nature of CAR activation and signaling through activation of other xenobiotic-activated receptors. PMID:25949234

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kearney, Sean Patrick

A hybrid fs/ps pure-rotational coherent anti-Stokes Raman scattering (CARS) scheme is systematically evaluated over a wide range of flame conditions in the product gases of two canonical flat-flame burners. Near-transform-limited, broadband femtosecond pump and Stokes pulses impulsively prepare a rotational Raman coherence, which is later probed using a high-energy, frequency-narrow picosecond beam generated by the second-harmonic bandwidth compression scheme that has recently been demonstrated for rotational CARS generation in H 2/air flat flames. The measured spectra are free of collision effects and nonresonant background and can be obtained on a single-shot basis at 1 kHz. The technique is evaluated formore » temperature/oxygen measurements in near-adiabatic H 2/air flames stabilized on the Hencken burner for equivalence ratios of φ = 0.20–1.20. Thermometry is demonstrated in hydrocarbon/air products for φ = 0.75–3.14 in premixed C 2H 4/air flat flames on the McKenna burner. Reliable spectral fitting is demonstrated for both shot-averaged and single-laser-shot data using a simple phenomenological model. Measurement accuracy is benchmarked by comparison to adiabatic-equilibrium calculations for the H 2/air flames, and by comparison with nanosecond CARS measurements for the C 2H 4/air flames. Quantitative accuracy comparable to nanosecond rotational CARS measurements is observed, while the observed precision in both the temperature and oxygen data is extraordinarily high, exceeding nanosecond CARS, and on par with the best published thermometric precision by femtosecond vibrational CARS in flames, and rotational femtosecond CARS at low temperature. Threshold levels of signal-to-noise ratio to achieve 1–2% precision in temperature and O 2/N 2 ratio are identified. Our results show that pure-rotational fs/ps CARS is a robust and quantitative tool when applied across a wide range of flame conditions spanning lean H 2/air combustion to fuel-rich sooting hydrocarbon flames.« less

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

PubMed Central

Eyquem, Justin; Mansilla-Soto, Jorge; Giavridis, Theodoros; van der Stegen, Sjoukje J. C.; Hamieh, Mohamad; Cunanan, Kristen M.; Odak, Ashlesha; Gönen, Mithat; Sadelain, Michel

2017-01-01

Chimeric antigen receptors (CARs) are synthetic receptors that redirect and reprogram T cells to mediate tumour rejection1. The most successful CARs used to date are those targeting CD19 (ref. 2), which offer the prospect of complete remission in patients with chemorefractory or relapsed B-cell malignancies3. CARs are typically transduced into the T cells of a patient using γ-retroviral4 vectors or other randomly integrating vectors5, which may result in clonal expansion, oncogenic transformation, variegated transgene expression and transcriptional silencing6–8. Recent advances in genome editing enable efficient sequence-specific interventions in human cells9,10, including targeted gene delivery to the CCR5 and AAVS1 loci11,12. Here we show that directing a CD19-specific CAR to the T-cell receptor α constant (TRAC) locus not only results in uniform CAR expression in human peripheral blood T cells, but also enhances T-cell potency, with edited cells vastly outperforming conventionally generated CAR T cells in a mouse model of acute lymphoblastic leukaemia. We further demonstrate that targeting the CAR to the TRAC locus averts tonic CAR signalling and establishes effective internalization and re-expression of the CAR following single or repeated exposure to antigen, delaying effector T-cell differentiation and exhaustion. These findings uncover facets of CAR immunobiology and underscore the potential of CRISPR/Cas9 genome editing to advance immunotherapies. PMID:28225754

CD28z CARs and Armored CARs

PubMed Central

Pegram, Hollie J.; Park, Jae H.; Brentjens, Renier J.

2015-01-01

CD19-targeted chimeric antigen receptor (CAR) T cells are currently being tested in the clinic with very promising outcomes. However, limitations to CAR T cell therapy exist. These include lack of efficacy against some tumors, specific targeting of tumor cells without affecting normal tissue and retaining activity within the suppressive tumor microenvironment. Whilst promising clinical trials are in progress, preclinical development is focused on optimizing CAR design, to generate “armored CAR T cells” which are protected from the inhibitory tumor microenvironment. Studies investigating the expression of cytokine transgenes, combination therapy with small molecule inhibitors or monoclonal antibodies are aimed at improving the anti-tumor efficacy of CAR T cell therapy. Other strategies aimed at improving CAR T cell therapy include utilizing dual CARs and chemokine receptors to more specifically target tumor cells. This review will describe the current clinical data and some novel “armored CAR T cell” approaches for improving anti-tumor efficacy therapy. PMID:24667958

CD28z CARs and armored CARs.

PubMed

Pegram, Hollie J; Park, Jae H; Brentjens, Renier J

2014-01-01

CD19-targeted chimeric antigen receptor (CAR) T cells are currently being tested in the clinic with very promising outcomes. However, limitations to CAR T cell therapy exist. These include lack of efficacy against some tumors, specific targeting of tumor cells without affecting normal tissue and retaining activity within the suppressive tumor microenvironment. Whereas promising clinical trials are in progress, preclinical development is focused on optimizing CAR design, to generate "armored CAR T cells," which are protected from the inhibitory tumor microenvironment. Studies investigating the expression of cytokine transgenes, combination therapy with small molecule inhibitors, or monoclonal antibodies, are aimed at improving the antitumor efficacy of CAR T cell therapy. Other strategies aimed at improving CAR T cell therapy include using dual CARs and chemokine receptors to more specifically target tumor cells. This review will describe the current clinical data and some novel armored CAR T cell approaches for improving antitumor efficacy therapy.

Rail-car impact tests with steel coil : car crush

DOT National Transportation Integrated Search

2003-04-22

Two grade-crossing impact tests were conducted in June 2002 at the Federal Railroad Administration's (FRA's) Transportation Technology Center in Pueblo, Colorado as part of the FRA's research into passenger equipment crashworthiness. In both of these...

40 CFR 201.23 - Test site, weather conditions and background noise criteria for measurement at a 30 meter (100...

Code of Federal Regulations, 2010 CFR

2010-07-01

... rail car operations and locomotive load cell test stands. 201.23 Section 201.23 Protection of... locomotive and rail car operations and locomotive load cell test stands. (a) The standard test site shall be... contribution from the operation of the load cell, if any, including load cell contribution during test. (h...

76 FR 47639 - Tesla Motors, Inc.; Receipt of Petition for Temporary Exemption From the Electronic Stability...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-05

... demonstrated that these systems reduce fatal single-vehicle crashes of passenger cars by 36 percent and fatal... the potential to prevent 70 percent of the fatal passenger car rollovers and 88 percent of the fatal..., the Roadster. Tesla began production of the all-electric Roadster in 2008 plans to conclude production...

Analysis of colliding vehicle interactions for the passenger rail train-to-train impact test

DOT National Transportation Integrated Search

2004-04-06

A full-scale train-to-train impact test was performed in : which a cab car-led passenger train traveling at 30 mph collided : with a standing locomotive-led train. During the test, the lead : cab car overrode the cab of the standing locomotive, susta...

Results of Energy-Loss Measurements on Passenger Car Tires Operating in the Free-Rolling and Braking/Traction Modes

DOT National Transportation Integrated Search

1980-01-01

This report described the results of tests on twelve passenger car tires performed on Calspan's Tire Research Facility (CTRF). The tests were used to measure energy loss under three different test conditions: 1) with the tire in a straight, free-roll...

Aerodynamic forces on freight trains : volume 1. wind tunnel tests of containers and trailers on flatcars

DOT National Transportation Integrated Search

1976-12-01

The aerodynamic forces on trailers and containers on flatcars have been measured in wind tunnel tests. The forces were measured on the central car of a five-car train consisting of a locomotive, three flatcars with various loadings and a boxcar. Test...

Train-to-Train Impact Test of Crash-Energy Management Passenger Rail Equipment: Occupant Experiments

DOT National Transportation Integrated Search

2006-11-06

As part of an ongoing passenger rail crashworthiness effort, : a full-scale impact test of a train with crash energy management : (CEM) passenger cars was conducted on March 23, 2006. In : this test, a train made up of a CEM cab car, four CEM coach :...

Prototype design and test of a collision protection system for cab car engineers.

DOT National Transportation Integrated Search

2014-12-01

Advancements in the design of rail cars can : potentially prevent the structural collapse of : space occupied by a cab car engineer : during a train collision. With adequate : survival space maintained, the next : crashworthiness objective is to mini...

Prototype design of a collision protection system for cab car engineers - fabrication and test.

DOT National Transportation Integrated Search

2015-06-01

Advancements in the structural crashworthiness of passenger rail cars now make it possible to preserve the compartmentalized : space occupied by a cab car engineer during a train collision. In order to translate this additional protection into improv...

Cars with antilock brakes no longer are overinvolved in fatal crashes

DOT National Transportation Integrated Search

2000-04-15

New tests suggest that cars with antilock braking system no longer are disproportionately involved in certain types of fatal crashes, but antilocks still are not producing reductions in overall fatal crash risk. Since the poor experience of cars with...

49 CFR 179.400-21 - Test of pressure relief valves.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Test of pressure relief valves. 179.400-21 Section... CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-21 Test of pressure relief valves. Each valve must be tested with air or gas for...

49 CFR 179.500-16 - Tests of pressure relief devices.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Tests of pressure relief devices. 179.500-16... CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.500-16 Tests of pressure relief devices. (a) Pressure relief valves shall be tested by air...

49 CFR 179.500-16 - Tests of pressure relief devices.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 3 2011-10-01 2011-10-01 false Tests of pressure relief devices. 179.500-16... CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.500-16 Tests of pressure relief devices. (a) Pressure relief valves shall be tested by air...

49 CFR 179.400-21 - Test of pressure relief valves.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Test of pressure relief valves. 179.400-21 Section... FOR TANK CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-21 Test of pressure relief valves. Each valve must be tested with air or gas...

49 CFR 179.500-16 - Tests of pressure relief devices.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 3 2012-10-01 2012-10-01 false Tests of pressure relief devices. 179.500-16... CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.500-16 Tests of pressure relief devices. (a) Pressure relief valves shall be tested by air...

49 CFR 179.500-16 - Tests of pressure relief devices.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 3 2014-10-01 2014-10-01 false Tests of pressure relief devices. 179.500-16... CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.500-16 Tests of pressure relief devices. (a) Pressure relief valves shall be tested by air...

The Cambridge Car Memory Test: a task matched in format to the Cambridge Face Memory Test, with norms, reliability, sex differences, dissociations from face memory, and expertise effects.

PubMed

Dennett, Hugh W; McKone, Elinor; Tavashmi, Raka; Hall, Ashleigh; Pidcock, Madeleine; Edwards, Mark; Duchaine, Bradley

2012-06-01

Many research questions require a within-class object recognition task matched for general cognitive requirements with a face recognition task. If the object task also has high internal reliability, it can improve accuracy and power in group analyses (e.g., mean inversion effects for faces vs. objects), individual-difference studies (e.g., correlations between certain perceptual abilities and face/object recognition), and case studies in neuropsychology (e.g., whether a prosopagnosic shows a face-specific or object-general deficit). Here, we present such a task. Our Cambridge Car Memory Test (CCMT) was matched in format to the established Cambridge Face Memory Test, requiring recognition of exemplars across view and lighting change. We tested 153 young adults (93 female). Results showed high reliability (Cronbach's alpha = .84) and a range of scores suitable both for normal-range individual-difference studies and, potentially, for diagnosis of impairment. The mean for males was much higher than the mean for females. We demonstrate independence between face memory and car memory (dissociation based on sex, plus a modest correlation between the two), including where participants have high relative expertise with cars. We also show that expertise with real car makes and models of the era used in the test significantly predicts CCMT performance. Surprisingly, however, regression analyses imply that there is an effect of sex per se on the CCMT that is not attributable to a stereotypical male advantage in car expertise.

Coherent anti-Stokes Raman scattering microscopy of single nanodiamonds

NASA Astrophysics Data System (ADS)

Pope, Iestyn; Payne, Lukas; Zoriniants, George; Thomas, Evan; Williams, Oliver; Watson, Peter; Langbein, Wolfgang; Borri, Paola

2014-11-01

Nanoparticles have attracted enormous attention for biomedical applications as optical labels, drug-delivery vehicles and contrast agents in vivo. In the quest for superior photostability and biocompatibility, nanodiamonds are considered one of the best choices due to their unique structural, chemical, mechanical and optical properties. So far, mainly fluorescent nanodiamonds have been utilized for cell imaging. However, their use is limited by the efficiency and costs in reliably producing fluorescent defect centres with stable optical properties. Here, we show that single non-fluorescing nanodiamonds exhibit strong coherent anti-Stokes Raman scattering (CARS) at the sp3 vibrational resonance of diamond. Using correlative light and electron microscopy, the relationship between CARS signal strength and nanodiamond size is quantified. The calibrated CARS signal in turn enables the analysis of the number and size of nanodiamonds internalized in living cells in situ, which opens the exciting prospect of following complex cellular trafficking pathways quantitatively.

Coherent anti-Stokes Raman scattering microscopy of single nanodiamonds

PubMed Central

Pope, Iestyn; Payne, Lukas; Zoriniants, George; Thomas, Evan; Williams, Oliver; Watson, Peter; Langbein, Wolfgang; Borri, Paola

2016-01-01

Nanoparticles have attracted enormous attention for biomedical applications as optical labels, drug delivery vehicles, and contrast agents in vivo. In the quest for superior photostability and bio-compatibility, nanodiamonds (NDs) are considered one of the best choices due to their unique structural, chemical, mechanical, and optical properties. So far, mainly fluorescent NDs have been utilized for cell imaging. However, their use is limited by the efficiency and costs in reliably producing fluorescent defect centers with stable optical properties. Here, we show that single non-fluorescing NDs exhibit strong coherent anti-Stokes Raman scattering (CARS) at the sp3 vibrational resonance of diamond. Using correlative light and electron microscopy, the relationship between CARS signal strength and ND size is quantified. The calibrated CARS signal in turn enables the analysis of the number and size of NDs internalized in living cells in situ, which opens the exciting prospect of following complex cellular trafficking pathways quantitatively. PMID:25305746

Coherent anti-Stokes Raman scattering microscopy of single nanodiamonds.

PubMed

Pope, Iestyn; Payne, Lukas; Zoriniants, George; Thomas, Evan; Williams, Oliver; Watson, Peter; Langbein, Wolfgang; Borri, Paola

2014-11-01

Nanoparticles have attracted enormous attention for biomedical applications as optical labels, drug-delivery vehicles and contrast agents in vivo. In the quest for superior photostability and biocompatibility, nanodiamonds are considered one of the best choices due to their unique structural, chemical, mechanical and optical properties. So far, mainly fluorescent nanodiamonds have been utilized for cell imaging. However, their use is limited by the efficiency and costs in reliably producing fluorescent defect centres with stable optical properties. Here, we show that single non-fluorescing nanodiamonds exhibit strong coherent anti-Stokes Raman scattering (CARS) at the sp(3) vibrational resonance of diamond. Using correlative light and electron microscopy, the relationship between CARS signal strength and nanodiamond size is quantified. The calibrated CARS signal in turn enables the analysis of the number and size of nanodiamonds internalized in living cells in situ, which opens the exciting prospect of following complex cellular trafficking pathways quantitatively.

Lane-changing behavior and its effect on energy dissipation using full velocity difference model

NASA Astrophysics Data System (ADS)

Wang, Jian; Ding, Jian-Xun; Shi, Qin; Kühne, Reinhart D.

2016-07-01

In real urban traffic, roadways are usually multilane with lane-specific velocity limits. Most previous researches are derived from single-lane car-following theory which in the past years has been extensively investigated and applied. In this paper, we extend the continuous single-lane car-following model (full velocity difference model) to simulate the three-lane-changing behavior on an urban roadway which consists of three lanes. To meet incentive and security requirements, a comprehensive lane-changing rule set is constructed, taking safety distance and velocity difference into consideration and setting lane-specific speed restriction for each lane. We also investigate the effect of lane-changing behavior on distribution of cars, velocity, headway, fundamental diagram of traffic and energy dissipation. Simulation results have demonstrated asymmetric lane-changing “attraction” on changeable lane-specific speed-limited roadway, which leads to dramatically increasing energy dissipation.

Vertical bending strength and torsional rigidity analysis of formula student car chassis

NASA Astrophysics Data System (ADS)

Hazimi, Hashfi; Ubaidillah, Setiyawan, Adi Eka Putra; Ramdhani, Hanief Cahya; Saputra, Murnanda Zaesy; Imaduddin, Fitrian

2018-02-01

Formula Society of Automotive Engineers (FSAE) is a competition for students to construct formula student car. One of an essential part of a formula student car is its chassis. Chassis is an internal vehicle frame which holds all another part of the vehicle and secures the driver. The team have to design their chassis and tests their design to achieve the best chassis that fulfill the regulation. This paper contains chassis design from Bengawan FSAE Team and some FEA tests to find out the Tensile Strength, Torsional Rigidity, and Von Misses Stress of Formula SAE car. Torsional rigidity was found by applying the static torsional test. The results from torsional rigidity test are a maximum deformation of 9.9512 mm with 1.7064 safety factor, and 35.935 MPa maximum Von Misses Stress. Moreover, then the result of the vertical bending strength test is 8.1214 mm max deformation with safety factor 4.2717, and 29.226 MPa maximum Von Misses Stress.

The peak velocity derived from the Carminatti Test is related to physical match performance in young soccer players.

PubMed

Fernandes-da-Silva, Juliano; Castagna, Carlo; Teixeira, Anderson Santiago; Carminatti, Lorival José; Guglielmo, Luiz Guilherme Antonacci

2016-12-01

The aim of this study was to examine the relationship between the peak velocity derived from the Carminatti Test (T-CAR) (PV T-CAR ) and physical match performance in young soccer players. Thirty-three youth soccer players were recruited from 2 non-professional clubs. Friendly matches and small-sided game were performed. Physical match demands were assessed using Global Positioning System (GPS) technology. On a separate occasion, the players were submitted to the T-CAR. Players were categorised into 3 groups based on their T-CAR performance: Low (PV T-CAR  ≤ P33), Intermediate (P33 > PV T-CAR  < P66) and High (PV T-CAR  ≥ P66). The PV T-CAR (15.5 ± 0.7 km·h -1 ) was significantly related to high-intensity activities (HIA; r = 0.78, P < 0.001), high-intensity running (HIR; r = 0.66, P < 0.001), sprinting (r = 0.62, P < 0.001) and total distance (TD) covered (r = 0.47, P < 0.01) during friendly matches. The PV T-CAR was strongly correlated with the amount of HIA (r = 0.81, P < 0.001), HIR (r = 0.85, P < 0.001) and TD covered (r = 0.81, P < 0.001) during small-sided game. No significant correlation was observed between the PV T-CAR and distance of sprinting (r = 0.49, P = 0.067) during small-side game. Furthermore, players in the High group covered significantly more TD (10%) and did more HIA (42%), sprinting (31%) and HIR (25%) during friendly matches compared to the players classified as having Low performance on the T-CAR. These differences still remained after adjusting for chronological age (CA), maturity and body size. In conclusion, the current study gives empirical support to the ecological and construct validity of this novel field test (T-CAR) as an indicator of match-related physical performance in young soccer players during pubertal years.

Expansion of the visual angle of a car rear-view image via an image mosaic algorithm

NASA Astrophysics Data System (ADS)

Wu, Zhuangwen; Zhu, Liangrong; Sun, Xincheng

2015-05-01

The rear-view image system is one of the active safety devices in cars and is widely applied in all types of vehicles and traffic safety areas. However, studies made by both domestic and foreign researchers were based on a single image capture device while reversing, so a blind area still remained to drivers. Even if multiple cameras were used to expand the visual angle of the car's rear-view image in some studies, the blind area remained because different source images were not mosaicked together. To acquire an expanded visual angle of a car rear-view image, two charge-coupled device cameras with optical axes angled at 30 deg were mounted below the left and right fenders of a car in three light conditions-sunny outdoors, cloudy outdoors, and an underground garage-to capture rear-view heterologous images of the car. Then these rear-view heterologous images were rapidly registered through the scale invariant feature transform algorithm. Combined with the random sample consensus algorithm, the two heterologous images were finally mosaicked using the linear weighted gradated in-and-out fusion algorithm, and a seamless and visual-angle-expanded rear-view image was acquired. The four-index test results showed that the algorithms can mosaic rear-view images well in the underground garage condition, where the average rate of correct matching was the lowest among the three conditions. The rear-view image mosaic algorithm presented had the best information preservation, the shortest computation time and the most complete preservation of the image detail features compared to the mean value method (MVM) and segmental fusion method (SFM), and it was also able to perform better in real time and provided more comprehensive image details than MVM and SFM. In addition, it had the most complete image preservation from source images among the three algorithms. The method introduced by this paper provided the basis for researching the expansion of the visual angle of a car rear-view image in all-weather conditions.

Hybrid fs/ps rotational CARS temperature and oxygen measurements in the product gases of canonical flat flames

DOE PAGES

Kearney, Sean Patrick

2014-12-31

A hybrid fs/ps pure-rotational coherent anti-Stokes Raman scattering (CARS) scheme is systematically evaluated over a wide range of flame conditions in the product gases of two canonical flat-flame burners. Near-transform-limited, broadband femtosecond pump and Stokes pulses impulsively prepare a rotational Raman coherence, which is later probed using a high-energy, frequency-narrow picosecond beam generated by the second-harmonic bandwidth compression scheme that has recently been demonstrated for rotational CARS generation in H 2/air flat flames. The measured spectra are free of collision effects and nonresonant background and can be obtained on a single-shot basis at 1 kHz. The technique is evaluated formore » temperature/oxygen measurements in near-adiabatic H 2/air flames stabilized on the Hencken burner for equivalence ratios of φ = 0.20–1.20. Thermometry is demonstrated in hydrocarbon/air products for φ = 0.75–3.14 in premixed C 2H 4/air flat flames on the McKenna burner. Reliable spectral fitting is demonstrated for both shot-averaged and single-laser-shot data using a simple phenomenological model. Measurement accuracy is benchmarked by comparison to adiabatic-equilibrium calculations for the H 2/air flames, and by comparison with nanosecond CARS measurements for the C 2H 4/air flames. Quantitative accuracy comparable to nanosecond rotational CARS measurements is observed, while the observed precision in both the temperature and oxygen data is extraordinarily high, exceeding nanosecond CARS, and on par with the best published thermometric precision by femtosecond vibrational CARS in flames, and rotational femtosecond CARS at low temperature. Threshold levels of signal-to-noise ratio to achieve 1–2% precision in temperature and O 2/N 2 ratio are identified. Our results show that pure-rotational fs/ps CARS is a robust and quantitative tool when applied across a wide range of flame conditions spanning lean H 2/air combustion to fuel-rich sooting hydrocarbon flames.« less

Documentation of deformation from passenger rail two-car impact test

DOT National Transportation Integrated Search

2003-11-01

The Volpe Center has been conducting research into rail equipment crashworthiness in support of the Federal Railroad Administrations (FRA) Office of Research and Development. As part of this research, full-scale crash tests of passenger cars have ...

Effects of Smoke-Free Laws on Alcohol-Related Car Crashes in California and New York: Time Series Analyses From 1982 to 2008

PubMed Central

Maldonado-Molina, Mildred; Hyland, Andrew; Wagenaar, Alexander C.

2013-01-01

We examined effects of New York and California’s statewide smoke-free restaurant and bar polices on alcohol-related car crash fatalities. We used an interrupted time-series design from 1982 to 2008, with 312 monthly observations, to examine the effect of each state’s law on single-vehicle-nighttime crashes and crashes involving a driver with a blood alcohol concentration of 0.08 grams per deciliter or greater. Implementation of New York and California’s statewide smoke-free policies was not associated with alcohol-related car crash fatalities. Additionally, analyses showed no effect of New York’s smoke-free policy on alcohol-related car crash fatalities in communities along the Pennsylvania-New York border. Statewide smoke-free restaurant and bar laws do not appear to affect rates of alcohol-related car crashes. PMID:23237160

Modified Ride-on Toy Cars for Early Power Mobility: A Technical Report

PubMed Central

Huang, Hsiang-han; Galloway, James C

2012-01-01

Background and Purpose Children with significantly decreased mobility have limited opportunities to explore their physical and social environment. A variety of assistive technologies are available to increase mobility, however no single device provides the level of functional mobility that typically developing children enjoy. The purpose of this technical report is to formally introduce a new power mobility option - the modified ride-on toy car. Key Points This report will provide a) an overview of toy car features, b) examples of basic electrical and mechanical modifications and c) a brief clinical case. Clinical Implications With creative use and customized modifications, toy cars function as a “general learning environment” for use in the clinic, home and school. As such, we anticipate that these cars will become a multi-use clinical tool to address not only mobility goals but also goals involving body function and structure such as posture and movement impairments. PMID:22466382

Modified ride-on toy cars for early power mobility: a technical report.

PubMed

Huang, Hsiang-Han; Galloway, James C

2012-01-01

Children with significantly decreased mobility have limited opportunities to explore their physical and social environment. A variety of assistive technologies are available to increase mobility; however, no single device provides the level of functional mobility that children developing typically enjoy. The purpose of this technical report is to formally introduce a new power mobility option--the modified ride-on toy car. This report will provide (a) an overview of toy car features, (b) examples of basic electrical and mechanical modifications, and (c) a brief clinical case. With creative use and customized modifications, toy cars can function as a "general learning environment" for use in the clinic, home, and school. As such, we anticipate that these cars will become a multiuse clinical tool to address not only mobility goals but also goals involving body function and structure such as posture and movement impairments.

Reduced food access due to a lack of money, inability to lift and lack of access to a car for food shopping: a multilevel study in Melbourne, Victoria.

PubMed

Burns, Cate; Bentley, Rebecca; Thornton, Lukar; Kavanagh, Anne

2011-06-01

To describe associations between demographic and individual and area-level socio-economic variables and restricted household food access due to lack of money, inability to lift groceries and lack of access to a car to do food shopping. Multilevel study of three measures of restricted food access, i.e. running out of money to buy food, inability to lift groceries and lack of access to a car for food shopping. Multilevel logistic regression was conducted to examine the risk of each of these outcomes according to demographic and socio-economic variables. Random selection of households from fifty small areas in Melbourne, Australia, in 2003. The main food shoppers in each household (n 2564). A lack of money was significantly more likely among the young and in households with single adults. Difficulty lifting was more likely among the elderly and those born overseas. The youngest and highest age groups both reported reduced car access, as did those born overseas and single-adult households. All three factors were most likely among those with a lower individual or household socio-economic position. Increased levels of area disadvantage were independently associated with difficulty lifting and reduced car access. In Melbourne, households with lower individual socio-economic position and area disadvantage have restricted access to food because of a lack of money and/or having physical limitations due difficulty lifting or lack of access to a car for food shopping. Further research is required to explore the relationship between physical restrictions and food access.

Assets among low-income families in the Great Recession

PubMed Central

Garfinkel, Irwin

2018-01-01

This paper examines the association between the Great Recession and real assets among families with young children. Real assets such as homes and cars are key indicators of economic well-being that may be especially valuable to low-income families. Using longitudinal data from the Fragile Families and Child Wellbeing Study (N = 4,898), we investigate the association between the city unemployment rate and home and car ownership and how the relationship varies by family structure (married, cohabiting, and single parents) and by race/ethnicity (White, Black, and Hispanic mothers). Using mother fixed-effects models, we find that a one percentage point increase in the unemployment rate is associated with a -0.5 percentage point decline in the probability of home ownership and a -0.7 percentage point decline in the probability of car ownership. We also find that the recession was associated with lower levels of home ownership for cohabiting families and for Hispanic families, as well as lower car ownership among single mothers and among Black mothers, whereas no change was observed among married families or White households. Considering that homes and cars are the most important assets among middle and low-income households in the U.S., these results suggest that the rise in the unemployment rate during the Great Recession may have increased household asset inequality across family structures and race/ethnicities, limiting economic mobility, and exacerbating the cycle of poverty. PMID:29401482

Assets among low-income families in the Great Recession.

PubMed

Duque, Valentina; Pilkauskas, Natasha V; Garfinkel, Irwin

2018-01-01

This paper examines the association between the Great Recession and real assets among families with young children. Real assets such as homes and cars are key indicators of economic well-being that may be especially valuable to low-income families. Using longitudinal data from the Fragile Families and Child Wellbeing Study (N = 4,898), we investigate the association between the city unemployment rate and home and car ownership and how the relationship varies by family structure (married, cohabiting, and single parents) and by race/ethnicity (White, Black, and Hispanic mothers). Using mother fixed-effects models, we find that a one percentage point increase in the unemployment rate is associated with a -0.5 percentage point decline in the probability of home ownership and a -0.7 percentage point decline in the probability of car ownership. We also find that the recession was associated with lower levels of home ownership for cohabiting families and for Hispanic families, as well as lower car ownership among single mothers and among Black mothers, whereas no change was observed among married families or White households. Considering that homes and cars are the most important assets among middle and low-income households in the U.S., these results suggest that the rise in the unemployment rate during the Great Recession may have increased household asset inequality across family structures and race/ethnicities, limiting economic mobility, and exacerbating the cycle of poverty.

KSC-2012-3033a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – At the NASA Railroad Yard at NASA’s Kennedy Space Center in Florida, preparations are under way for the departure of a train made up of tank cars. The railroad’s track runs past Kennedy’s 525-foot-tall Vehicle Assembly Building in the background. The train is headed for the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3032a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – At the NASA Railroad Yard at NASA’s Kennedy Space Center in Florida, preparations are under way for the departure of a train made up of tank cars. The train will pass by Kennedy’s 525-foot-tall Vehicle Assembly Building in the background. The train is headed for the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Collision safety comparison of conventional and crash energy management passenger rail car designs

DOT National Transportation Integrated Search

2003-04-22

In conjunction with full-scale equipment tests, collision dynamics models of passenger rail cars have been developed to investigate the benefits provided by incorporating energy-absorbing crush zones at the ends of the cars. In a collision, the major...

Unexpected Attraction of Polarotactic Water-Leaving Insects to Matt Black Car Surfaces: Mattness of Paintwork Cannot Eliminate the Polarized Light Pollution of Black Cars

PubMed Central

Blaho, Miklos; Herczeg, Tamas; Kriska, Gyorgy; Egri, Adam; Szaz, Denes; Farkas, Alexandra; Tarjanyi, Nikolett; Czinke, Laszlo; Barta, Andras; Horvath, Gabor

2014-01-01

The horizontally polarizing surface parts of shiny black cars (the reflection-polarization characteristics of which are similar to those of water surfaces) attract water-leaving polarotactic insects. Thus, shiny black cars are typical sources of polarized light pollution endangering water-leaving insects. A new fashion fad is to make car-bodies matt black or grey. Since rough (matt) surfaces depolarize the reflected light, one of the ways of reducing polarized light pollution is to make matt the concerned surface. Consequently, matt black/grey cars may not induce polarized light pollution, which would be an advantageous feature for environmental protection. To test this idea, we performed field experiments with horizontal shiny and matt black car-body surfaces laid on the ground. Using imaging polarimetry, in multiple-choice field experiments we investigated the attractiveness of these test surfaces to various water-leaving polarotactic insects and obtained the following results: (i) The attractiveness of black car-bodies to polarotactic insects depends in complex manner on the surface roughness (shiny, matt) and species (mayflies, dolichopodids, tabanids). (ii) Non-expectedly, the matt dark grey car finish is much more attractive to mayflies (being endangered and protected in many countries) than matt black finish. (iii) The polarized light pollution of shiny black cars usually cannot be reduced with the use of matt painting. On the basis of these, our two novel findings are that (a) matt car-paints are highly polarization reflecting, and (b) these matt paints are not suitable to repel polarotactic insects. Hence, the recent technology used to make matt the car-bodies cannot eliminate or even can enhance the attractiveness of black/grey cars to water-leaving insects. Thus, changing shiny black car painting to matt one is a disadvantageous fashion fad concerning the reduction of polarized light pollution of black vehicles. PMID:25076137

Unexpected attraction of polarotactic water-leaving insects to matt black car surfaces: mattness of paintwork cannot eliminate the polarized light pollution of black cars.

PubMed

Blaho, Miklos; Herczeg, Tamas; Kriska, Gyorgy; Egri, Adam; Szaz, Denes; Farkas, Alexandra; Tarjanyi, Nikolett; Czinke, Laszlo; Barta, Andras; Horvath, Gabor

2014-01-01

The horizontally polarizing surface parts of shiny black cars (the reflection-polarization characteristics of which are similar to those of water surfaces) attract water-leaving polarotactic insects. Thus, shiny black cars are typical sources of polarized light pollution endangering water-leaving insects. A new fashion fad is to make car-bodies matt black or grey. Since rough (matt) surfaces depolarize the reflected light, one of the ways of reducing polarized light pollution is to make matt the concerned surface. Consequently, matt black/grey cars may not induce polarized light pollution, which would be an advantageous feature for environmental protection. To test this idea, we performed field experiments with horizontal shiny and matt black car-body surfaces laid on the ground. Using imaging polarimetry, in multiple-choice field experiments we investigated the attractiveness of these test surfaces to various water-leaving polarotactic insects and obtained the following results: (i) The attractiveness of black car-bodies to polarotactic insects depends in complex manner on the surface roughness (shiny, matt) and species (mayflies, dolichopodids, tabanids). (ii) Non-expectedly, the matt dark grey car finish is much more attractive to mayflies (being endangered and protected in many countries) than matt black finish. (iii) The polarized light pollution of shiny black cars usually cannot be reduced with the use of matt painting. On the basis of these, our two novel findings are that (a) matt car-paints are highly polarization reflecting, and (b) these matt paints are not suitable to repel polarotactic insects. Hence, the recent technology used to make matt the car-bodies cannot eliminate or even can enhance the attractiveness of black/grey cars to water-leaving insects. Thus, changing shiny black car painting to matt one is a disadvantageous fashion fad concerning the reduction of polarized light pollution of black vehicles.

KSC-2012-3053

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train crosses the railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3052

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train crosses the railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3034a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – Engineers board a NASA Railroad train in preparation for its departure from the NASA Railroad Yard at NASA’s Kennedy Space Center in Florida. The train is headed for the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3039a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train passes in front of the 525-foot-tall Vehicle Assembly Building at NASA’s Kennedy Space Center in Florida. At the far right is the Orbiter Processing Facility. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2889

NASA Image and Video Library

2012-05-21

CAPE CANAVERAL, Fla. – At the NASA Railroad yard at Kennedy Space Center in Florida, cranes are enlisted to lift helium tank cars from their trucks onto flat cars in preparation for a journey to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s tank cars will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The yard is located in Kennedy’s Launch Complex 39 near the 525-foot-tall Vehicle Assembly Building, in the background. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3036a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train passes in front of the twin bays of the Orbiter Processing Facility at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3035a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train passes in front of the twin bays of the Orbiter Processing Facility at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3056

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train has crossed the Indian River on the railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3050

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train crosses the railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3051

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train crosses the railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Acoustic detection of rail car roller bearing defects. Phase III, System evaluation test.

DOT National Transportation Integrated Search

2003-08-01

In July 1999, Transportation Technology Center, Inc. (TTCI), a subsidiary of the Association of American Railroads (AAR), conducted a system evaluation test as part of the Federal Railroad Administrations (FRA) Improved Freight Car Roller Bearing ...

14. VIEW TO WESTSOUTHWEST ACROSS CAR TEST TRACT SITE TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. VIEW TO WEST-SOUTHWEST ACROSS CAR TEST TRACT SITE TO NORTH END OF ASSEMBLY PLANT, SHOWING NORTH END ADDITIONS. - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Implementation of object-oriented programming in study of electrical race car

NASA Astrophysics Data System (ADS)

Nowak, M.; Baier, M.

2016-08-01

The paper covers issue of conducting advanced research of electrical race car participating in international competition called Sileverline Corporate Challenge. Process of designing race cars in Silesian Greenpower team is aided by a professional engine test stand built particularly in purpose of this research. Phase of testing and simulation is an important part of the implementation of new technologies. Properly developed solutions and test procedures are able to significantly shorten development time and reduce design costs. Testing process must be controlled by a modular and flexible application, easy to modify and ensuring safety. This paper describes the concept of object-oriented programming in LabVIEW and exemplary architecture of object-oriented control application designed to control engine test stand of the electrical race car. Eventually, the task of application will be to steer the electromagnetic brake and the engine load torque to perform according to data from the actual race track. During the designing process of the car, minimizing energy losses and maximizing powertrain efficiency are the main aspects taken into consideration. One of the crucial issues to accomplish these goals is to maintain optimal performance of the motor by applying effective cooling. The paper covers the research verifying the effectiveness of the cooling system.

49 CFR 179.220-23 - Test of tanks.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Test of tanks. 179.220-23 Section 179.220-23... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.220-23 Test of tanks. (a) Each inner container...

49 CFR 179.220-24 - Tests of pressure relief valves.

Code of Federal Regulations, 2012 CFR

2012-10-01

... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.220-24 Tests of pressure relief valves. Each safety relief valve must be tested by air or gas for compliance with § 179.15...

49 CFR 179.220-24 - Tests of pressure relief valves.

Code of Federal Regulations, 2011 CFR

2011-10-01

... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.220-24 Tests of pressure relief valves. Each safety relief valve must be tested by air or gas for compliance with § 179.15...

49 CFR 179.500-16 - Tests of pressure relief devices.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Tests of pressure relief devices. 179.500-16... FOR TANK CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.500-16 Tests of pressure relief devices. (a) Pressure relief valves shall be...

A fully human chimeric antigen receptor with potent activity against cancer cells but reduced risk for off-tumor toxicity

PubMed Central

Song, De-Gang; Ye, Qunrui; Poussin, Mathilde; Liu, Lin; Figini, Mariangela; Powell, Daniel J.

2015-01-01

Chimeric antigen receptors (CARs) can redirect T cells against antigen-expressing tumors in an HLA-independent manner. To date, various CARs have been constructed using mouse single chain antibody variable fragments (scFvs) of high affinity that are immunogenic in humans and have the potential to mediate “on-target” toxicity. Here, we developed and evaluated a fully human CAR comprised of the human C4 folate receptor-alpha (αFR)-specific scFv coupled to intracellular T cell signaling domains. Human T cells transduced to express the C4 CAR specifically secreted proinflammatory cytokine and exerted cytolytic functions when cultured with αFR-expressing tumors in vitro. Adoptive transfer of C4 CAR T cells mediated the regression of large, established human ovarian cancer in a xenogeneic mouse model. Relative to a murine MOv19 scFv-based αFR CAR, C4 CAR T cells mediated comparable cytotoxic tumor activity in vitro and in vivo but had lower affinity for αFR protein and exhibited reduced recognition of normal cells expressing low levels of αFR. Thus, T cells expressing a fully human CAR of intermediate affinity can efficiently kill antigen-expressing tumors in vitro and in vivo and may overcome issues of transgene immunogenicity and “on-target off-tumor” toxicity that plague trials utilizing CARs containing mouse-derived, high affinity scFvs. PMID:26101914

The Cortisol Awakening Response and Cognition across the Adult Lifespan

PubMed Central

Ennis, Gilda E.; Moffat, Scott D.; Hertzog, Christopher

2016-01-01

Although the hippocampus is thought to play a central role in the regulation of the cortisol awakening response (CAR), results from past studies examining the relationship between the CAR and hippocampally-mediated memory and cognition have been mixed. Inconsistent findings may be due to the use of cortisol samples collected on only 1 to 2 days since reduced sampling can permit unstable situational factors to bias results. We used cortisol assessments from 10 consecutive days to test the relationship of the CAR to episodic memory, working memory, and processing speed in a sample of healthy young, middle-aged, and older adults (age range: 23 – 79 years; N = 56). We tested if the relationship between the CAR and cognition would depend upon age and also tested if other cortisol measures, specifically waking cortisol, diurnal cortisol output (i.e., area under the curve) and diurnal cortisol slope (linear and quadratic), would be related to cognition. We found that a more positive CAR slope was related to better episodic memory and that this relationship did not depend upon age. The CAR was not significantly related to working memory. The relationship of the CAR to processing speed was not significant when using a CAR measure that corrected for non-compliant cortisol sampling. We also found that higher waking cortisol was significantly related to better working memory, but not episodic memory or processing speed. Neither diurnal cortisol output nor diurnal linear cortisol slope was significantly related to cognitive functioning. Future work should investigate the mechanisms underpinning the relationship of the cortisol awakening process to cognitive functioning. PMID:27105036

Analysis of the car body stability performance after coupler jack-knifing during braking

NASA Astrophysics Data System (ADS)

Guo, Lirong; Wang, Kaiyun; Chen, Zaigang; Shi, Zhiyong; Lv, Kaikai; Ji, Tiancheng

2018-06-01

This paper aims to improve car body stability performance by optimising locomotive parameters when coupler jack-knifing occurs during braking. In order to prevent car body instability behaviour caused by coupler jack-knifing, a multi-locomotive simulation model and a series of field braking tests are developed to analyse the influence of the secondary suspension and the secondary lateral stopper on the car body stability performance during braking. According to simulation and test results, increasing secondary lateral stiffness contributes to limit car body yaw angle during braking. However, it seriously affects the dynamic performance of the locomotive. For the secondary lateral stopper, its lateral stiffness and free clearance have a significant influence on improving the car body stability capacity, and have less effect on the dynamic performance of the locomotive. An optimised measure was proposed and adopted on the test locomotive. For the optimised locomotive, the lateral stiffness of secondary lateral stopper is increased to 7875 kN/m, while its free clearance is decreased to 10 mm. The optimised locomotive has excellent dynamic and safety performance. Comparing with the original locomotive, the maximum car body yaw angle and coupler rotation angle of the optimised locomotive were reduced by 59.25% and 53.19%, respectively, according to the practical application. The maximum derailment coefficient was 0.32, and the maximum wheelset lateral force was 39.5 kN. Hence, reasonable parameters of secondary lateral stopper can improve the car body stability capacity and the running safety of the heavy haul locomotive.

Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer

PubMed Central

Pyo, Suhkneung; Kang, Chung Hyo; Lee, Chong Ock; Lee, Heung Kyoung; Choi, Sang Un; Park, Chi Hoon

2018-01-01

Gastric cancer is a malignancy that has a high mortality rate. Although progress has been made in the treatment of gastric cancer, many patients experience cancer recurrence and metastasis. Folate receptor 1 (FOLR1) is overexpressed on the cell surface in over one-third of gastric cancer patients, but rarely is expressed in normal tissue. This makes FOLR1 a potential target for chimeric antigen receptor (CAR) T cell immunotherapy, although the function of FOLR1 has not been elucidated. CAR are engineered fusion receptor composed of an antigen recognition region and signaling domains. T cells expressing CAR have specific activation and cytotoxic effects against cancer cells containing the target antigen. In this study, we generated a CAR that targets FOLR1 composed of a single-chain variable fragment (scFv) of FOLR1 antibody and signaling domains consisting of CD28 and CD3ζ. Both FOLR1-CAR KHYG-1, a natural killer cell line, and FOLR1-CAR T cells recognized FOLR1-positive gastric cancer cells in a MHC-independent manner and induced secretion of various cytokines and caused cell death. Conclusively, this is the first study to demonstrate that CAR KHYG-1/T cells targeting FOLR1 are effective against FOLR1-positive gastric cancer cells. PMID:29874279

Immunological quality and performance of tumor vessel-targeting CAR-T cells prepared by mRNA-EP for clinical research

PubMed Central

Inoo, Kanako; Inagaki, Ryo; Fujiwara, Kento; Sasawatari, Shigemi; Kamigaki, Takashi; Nakagawa, Shinsaku; Okada, Naoki

2016-01-01

We previously reported that tumor vessel-redirected T cells, which were genetically engineered with chimeric antigen receptor (CAR) specific for vascular endothelial growth factor receptor 2 (VEGFR2), demonstrated significant antitumor effects in various murine solid tumor models. In the present study, we prepared anti-VEGFR2 CAR-T cells by CAR-coding mRNA electroporation (mRNA-EP) and analyzed their immunological characteristics and functions for use in clinical research. The expression of anti-VEGFR2 CAR on murine and human T cells was detected with approximately 100% efficiency for a few days, after peaking 6–12 hours after mRNA-EP. Triple transfer of murine anti-VEGFR2 CAR-T cells into B16BL6 tumor-bearing mice demonstrated an antitumor effect comparable to that for the single transfer of CAR-T cells engineered with retroviral vector. The mRNA-EP did not cause any damage or defects to human T-cell characteristics, as determined by viability, growth, and phenotypic parameters. Additionally, two kinds of human anti-VEGFR2 CAR-T cells, which expressed different CAR construction, differentiated to effector phase with cytokine secretion and cytotoxic activity in antigen-specific manner. These results indicate that our anti-VEGFR2 CAR-T cells prepared by mRNA-EP have the potential in terms of quality and performance to offer the prospect of safety and efficacy in clinical research as cellular medicine. PMID:27909701

Adnectin-Based Design of Chimeric Antigen Receptor for T Cell Engineering.

PubMed

Han, Xiaolu; Cinay, Gunce E; Zhao, Yifan; Guo, Yunfei; Zhang, Xiaoyang; Wang, Pin

2017-11-01

Although chimeric antigen receptor (CAR)-engineered T cell therapy has achieved encouraging clinical trial results for treating hematological cancers, further optimization can likely expand this therapeutic success to more patients and other cancer types. Most CAR constructs used in clinical trials incorporate single chain variable fragment (scFv) as the extracellular antigen recognition domain. The immunogenicity of nonhuman scFv could cause host rejection against CAR T cells and compromise their persistence and efficacy. The limited availability of scFvs and slow discovery of new monoclonal antibodies also limit the development of novel CAR constructs. Adnectin, a class of affinity molecules derived from the tenth type III domain of human fibronectin, can be an alternative to scFv as an antigen-binding moiety in the design of CAR molecules. We constructed adnectin-based CARs targeting epithelial growth factor receptor (EGFR) and found that compared to scFv-based CAR, T cells engineered with adnectin-based CARs exhibited equivalent cell-killing activity against target H292 lung cancer cells in vitro and had comparable antitumor efficacy in xenograft tumor-bearing mice in vivo. In addition, with optimal affinity tuning, adnectin-based CAR showed higher selectivity on target cells with high EGFR expression than on those with low expression. This new design of adnectin CARs can potentially facilitate the development of T cell immunotherapy for cancer and other diseases. Copyright © 2017 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.

Targeting Fibroblast Activation Protein in Tumor Stroma with Chimeric Antigen Receptor T Cells Can Inhibit Tumor Growth and Augment Host Immunity Without Severe Toxicity

PubMed Central

Wang, Liang-Chuan S; Lo, Albert; Scholler, John; Sun, Jing; Majumdar, Rajrupa S; Kapoor, Veena; Antzis, Michael; Cotner, Cody E.; Johnson, Laura A; Durham, Amy C; Solomides, Charalambos C.; June, Carl H; Puré, Ellen; Albelda, Steven M

2013-01-01

The majority of chimeric antigen receptor (CAR) T cell research has focused on attacking cancer cells. Here we show that targeting the tumor-promoting, non-transformed stromal cells using CAR T cells may offer several advantages. We developed a retroviral CAR construct specific for the mouse fibroblast activation protein (FAP), comprising a single chain Fv FAP (mAb 73.3) with the CD8α hinge and transmembrane regions, and the human CD3ζ and 4-1BB activation domains. The transduced muFAP-CAR mouse T cells secreted IFNγ and killed FAP-expressing 3T3 target cells specifically. Adoptively transferred 73.3-FAP-CAR mouse T cells selectively reduced FAPhi stromal cells and inhibited the growth of multiple types of subcutaneously transplanted tumors in wild-type, but not FAP-null immune-competent syngeneic mice. The antitumor effects could be augmented by multiple injections of the CAR T cells, by using CAR T cells with a deficiency in diacylglycerol kinase, or by combination with a vaccine. A major mechanism of action of the muFAP-CAR T cells was the augmentation of the endogenous CD8+ T cell antitumor responses. Off-tumor toxicity in our models was minimal following muFAP-CAR T cell therapy. In summary, inhibiting tumor growth by targeting tumor stroma with adoptively transferred CAR T cells directed to FAP can be safe and effective suggesting that further clinical development of anti-human FAP-CAR is warranted. PMID:24778279

Triplet Excitation Transfer between Carotenoids in the LH2 Complex from Photosynthetic Bacterium Rhodopseudomonas palustris.

PubMed

Feng, Juan; Wang, Qian; Wu, Yi-Shi; Ai, Xi-Cheng; Zhang, Xu-Jia; Huang, You-Guo; Zhang, Xing-Kang; Zhang, Jian-Ping

2004-01-01

We have studied, by means of sub-microsecond time-resolved absorption spectroscopy, the triplet-excited state dynamics of carotenoids (Cars) in the intermediate-light adapted LH2 complex (ML-LH2) from Rhodopseudomonas palustris containing Cars with different numbers of conjugated double bonds. Following pulsed photo-excitation at 590 nm at room temperature, rapid spectral equilibration was observed either as a red shift of the isosbestic wavelength on a time scale of 0.6-1.0 mus, or as a fast decay in the shorter-wavelength side of the T(n)<--T(1) absorption of Cars with a time constant of 0.5-0.8 mus. Two major spectral components assignable to Cars with 11 and 12 conjugated double bonds were identified. The equilibration was not observed in the ML-LH2 at 77 K, or in the LH2 complex from Rhodobacter sphaeroides G1C containing a single type of Car. The unique spectral equilibration was ascribed to temperature-dependent triplet excitation transfer among different Car compositions. The results suggest that Cars of 11 and 12 conjugated bonds, both in close proximity of BChls, may coexist in an alpha,beta-subunit of the ML-LH2 complex.

CAR T Cells Targeting Podoplanin Reduce Orthotopic Glioblastomas in Mouse Brains.

PubMed

Shiina, Satoshi; Ohno, Masasuke; Ohka, Fumiharu; Kuramitsu, Shunichiro; Yamamichi, Akane; Kato, Akira; Motomura, Kazuya; Tanahashi, Kuniaki; Yamamoto, Takashi; Watanabe, Reiko; Ito, Ichiro; Senga, Takeshi; Hamaguchi, Michinari; Wakabayashi, Toshihiko; Kaneko, Mika K; Kato, Yukinari; Chandramohan, Vidyalakshmi; Bigner, Darell D; Natsume, Atsushi

2016-03-01

Glioblastoma (GBM) is the most common and lethal primary malignant brain tumor in adults with a 5-year overall survival rate of less than 10%. Podoplanin (PDPN) is a type I transmembrane mucin-like glycoprotein, expressed in the lymphatic endothelium. Several solid tumors overexpress PDPN, including the mesenchymal type of GBM, which has been reported to present the worst prognosis among GBM subtypes. Chimeric antigen receptor (CAR)-transduced T cells can recognize predefined tumor surface antigens independent of MHC restriction, which is often downregulated in gliomas. We constructed a lentiviral vector expressing a third-generation CAR comprising a PDPN-specific antibody (NZ-1-based single-chain variable fragment) with CD28, 4-1BB, and CD3ζ intracellular domains. CAR-transduced peripheral blood monocytes were immunologically evaluated by calcein-mediated cytotoxic assay, ELISA, tumor size, and overall survival. The generated CAR T cells were specific and effective against PDPN-positive GBM cells in vitro. Systemic injection of the CAR T cells into an immunodeficient mouse model inhibited the growth of intracranial glioma xenografts in vivo. CAR T-cell therapy that targets PDPN would be a promising adoptive immunotherapy to treat mesenchymal GBM. ©2016 American Association for Cancer Research.

Phase I clinical trial will test multi-targeted immunotherapy in common childhood cancer | Center for Cancer Research

Cancer.gov

Chimeric antigen receptor (CAR) T-cell immunotherapy targeting the protein CD19 has shown promise in treating acute lymphoblastic leukemia (ALL). CD22-CAR T-cell therapy has yielded similarly encouraging results, but many patients relapse after either therapy. In an upcoming phase I clinical trial, Center for Cancer Research investigators will test a new strategy—treating patients with a CAR T-cell therapy that targets CD19 and CD22 simultaneously.

Fatigue crack growth equations for TC-128B tank car steel

DOT National Transportation Integrated Search

2006-10-01

In an effort to develop relevant data for use in applying damage tolerance analysis concepts to railroad tank cars, the fatigue crack growth (FCG) behavior of TC-128B tank car steel was investigated by SwRI in a previous test program conducted for th...

A crush zone design for an existing passenger rail cab car

DOT National Transportation Integrated Search

2005-11-05

A Crash Energy Management (CEM) cab car crush zone : design has been developed for retrofit onto an existing Budd : M1 cab car. This design is to be used in the upcoming fullscale : train-to-train test of a CEM consist impacting a standing : freight ...

A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in Phycomyces

PubMed Central

Arrach, Nabil; Fernández-Martín, Rafael; Cerdá-Olmedo, Enrique; Avalos, Javier

2001-01-01

Previous complementation and mapping of mutations that change the usual yellow color of the Zygomycete Phycomyces blakesleeanus to white or red led to the definition of two structural genes for carotene biosynthesis. We have cloned one of these genes, carRA, by taking advantage of its close linkage to the other, carB, responsible for phytoene dehydrogenase. The sequences of the wild type and six mutants have been established, compared with sequences in other organisms, and correlated with the mutant phenotypes. The carRA and carB coding sequences are separated by 1,381 untranslated nucleotides and are divergently transcribed. Gene carRA contains separate domains for two enzymes, lycopene cyclase and phytoene synthase, and regulates the overall activity of the pathway and its response to physical and chemical stimuli from the environment. The lycopene cyclase domain of carRA derived from a duplication of a gene from a common ancestor of fungi and Brevibacterium linens; the phytoene synthase domain is similar to the phytoene and squalene synthases of many organisms; but the regulatory functions appear to be specific to Phycomyces. PMID:11172012

Driving CAR-Based T-Cell Therapy to Success

PubMed Central

Jena, Bipulendu; Moyes, Judy S; Huls, Helen; Cooper, Laurence JN

2014-01-01

T-cells that have been genetically modified, activated, and propagated ex vivo can be infused to control tumor progression in patients who are refractory to conventional treatments. Early-phase clinical trials demonstrate that the tumor-associated antigen (TAA) CD19 can be therapeutically engaged through the enforced expression of a chimeric antigen receptor (CAR) on clinical-grade T-cells. Advances in vector design, the architecture of the CAR molecule especially as associated with T-cell co-stimulatory pathways, and understanding of the tumor microenvironment, play significant roles in the successful treatment of medically fragile patients. However, some recipients of CAR+ T-cells demonstrate incomplete responses. Understanding the potential for treatment failure provides a pathway to improve the potency of adoptive transfer of CAR+ T-cells. High throughput single-cell analyses to understand the complexity of the inoculum coupled with animal models may provide insight into the therapeutic potential of genetically modified T-cells. This review focusses on recent advances regarding the human application of C19-specific CAR+ T-cells and explores how their success for hematologic cancers can provide a framework for investigational treatment of solid tumor malignancies. PMID:24488441

Neural correlates of culturally familiar brands of car manufacturers.

PubMed

Schaefer, Michael; Berens, Harald; Heinze, Hans-Jochen; Rotte, Michael

2006-06-01

Brands have a high impact on people's economic decisions. People may prefer products of brands even among almost identical products. Brands can be defined as cultural-based symbols, which promise certain advantages of a product. Recent studies suggest that the prefrontal cortex may be crucial for the processing of brand knowledge. The aim of this study was to examine the neural correlates of culturally based brands. We confronted subjects with logos of car manufactures during an fMRI session and instructed them to imagine and use a car of these companies. As a control condition, we used graphically comparable logos of car manufacturers that were unfamiliar to the culture of the subjects participating in this study. If they did not know the logo of the brand, they were told to imagine and use a generic car. Results showed activation of a single region in the medial prefrontal cortex related to the logos of the culturally familiar brands. We discuss the results as self-relevant processing induced by the imagined use of cars of familiar brands and suggest that the prefrontal cortex plays a crucial role for processing culturally based brands.

49 CFR 179.400-18 - Test of inner tank.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Test of inner tank. 179.400-18 Section 179.400-18... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-18 Test of inner tank...

49 CFR 238.317 - Class II brake test.

Code of Federal Regulations, 2014 CFR

2014-10-01

....315(a)(1); (3) When previously tested units (i.e., cars that received a Class I brake test within the... hours) are added to the train; (4) When cars or equipment are removed from the train; and (5) When an... locomotives that utilize an electric signal to communicate a service brake application and only a pneumatic...

49 CFR 238.317 - Class II brake test.

Code of Federal Regulations, 2012 CFR

2012-10-01

....315(a)(1); (3) When previously tested units (i.e., cars that received a Class I brake test within the... hours) are added to the train; (4) When cars or equipment are removed from the train; and (5) When an... locomotives that utilize an electric signal to communicate a service brake application and only a pneumatic...

49 CFR 238.317 - Class II brake test.

Code of Federal Regulations, 2013 CFR

2013-10-01

....315(a)(1); (3) When previously tested units (i.e., cars that received a Class I brake test within the... hours) are added to the train; (4) When cars or equipment are removed from the train; and (5) When an... locomotives that utilize an electric signal to communicate a service brake application and only a pneumatic...

49 CFR 238.317 - Class II brake test.

Code of Federal Regulations, 2010 CFR

2010-10-01

....315(a)(1); (3) When previously tested units (i.e., cars that received a Class I brake test within the... hours) are added to the train; (4) When cars or equipment are removed from the train; and (5) When an... locomotives that utilize an electric signal to communicate a service brake application and only a pneumatic...

49 CFR 238.317 - Class II brake test.

Code of Federal Regulations, 2011 CFR

2011-10-01

....315(a)(1); (3) When previously tested units (i.e., cars that received a Class I brake test within the... hours) are added to the train; (4) When cars or equipment are removed from the train; and (5) When an... locomotives that utilize an electric signal to communicate a service brake application and only a pneumatic...

40 CFR 201.24 - Procedures for measurement at a 30 meter (100 feet) distance of the noise from locomotive and...

Code of Federal Regulations, 2010 CFR

2010-07-01

... meter (100 feet) distance of the noise from locomotive and rail car operations and locomotive load cell... locomotive and rail car operations and locomotive load cell test stands. (a) Microphone positions. (1) The... measured. (b) Stationary locomotive and locomotive load cell test stand tests. (1) For stationary...

40 CFR 201.24 - Procedures for measurement at a 30 meter (100 feet) distance of the noise from locomotive and...

Code of Federal Regulations, 2011 CFR

2011-07-01

... meter (100 feet) distance of the noise from locomotive and rail car operations and locomotive load cell... locomotive and rail car operations and locomotive load cell test stands. (a) Microphone positions. (1) The... measured. (b) Stationary locomotive and locomotive load cell test stand tests. (1) For stationary...

40 CFR 201.24 - Procedures for measurement at a 30 meter (100 feet) distance of the noise from locomotive and...

Code of Federal Regulations, 2012 CFR

2012-07-01

... meter (100 feet) distance of the noise from locomotive and rail car operations and locomotive load cell... locomotive and rail car operations and locomotive load cell test stands. (a) Microphone positions. (1) The... measured. (b) Stationary locomotive and locomotive load cell test stand tests. (1) For stationary...

40 CFR 201.24 - Procedures for measurement at a 30 meter (100 feet) distance of the noise from locomotive and...

Code of Federal Regulations, 2014 CFR

2014-07-01

... meter (100 feet) distance of the noise from locomotive and rail car operations and locomotive load cell... locomotive and rail car operations and locomotive load cell test stands. (a) Microphone positions. (1) The... measured. (b) Stationary locomotive and locomotive load cell test stand tests. (1) For stationary...

40 CFR 201.24 - Procedures for measurement at a 30 meter (100 feet) distance of the noise from locomotive and...

Code of Federal Regulations, 2013 CFR

2013-07-01

... meter (100 feet) distance of the noise from locomotive and rail car operations and locomotive load cell... locomotive and rail car operations and locomotive load cell test stands. (a) Microphone positions. (1) The... measured. (b) Stationary locomotive and locomotive load cell test stand tests. (1) For stationary...

[Reaction time of drivers who caused road traffic accidents].

PubMed

Durić, Predrag; Filipović, Danka

2009-01-01

Human factor is the single cause of road traffic injuries in 57%, and together with other factors in more than 90% of all road traffic accidents. Human factor includes many aspects, where reaction time is very important. Thirty healthy drivers 28-40 y.o. with 50-500 km passed per week, having caused at least one road traffic accident in the last ten years were selected, provided they were not under the influence of alcohol and drugs during traffic accident. The same number of control were selected. Both cases and controls were tested to reaction time. We found statistically significant difference between car drivers who caused car accidents and those who did not in both simple and choice reaction times. Car drivers who caused road traffic accidents have longer reaction time (both simple and choice reaction time), but as the tasks were more complex, that difference was less visible. Since drivers involved in this study had introductory phase before measuring their reaction times, they faced with unpleasant sound when they made mistake, which forced them to be aware not to make a mistake in further tasks, so they showed longer reaction times. Measuring of reaction time seems to be important, and as we have showed they are different in drivers who have caused road traffic accidents and those who have do not.

Analysis of car-to-bicycle approach patterns for developing active safety devices.

PubMed

Matsui, Yasuhiro; Oikawa, Shoko; Hitosugi, Masahito

2016-05-18

To reduce the severity of injuries and the number of cyclist deaths in traffic accidents, active safety devices providing cyclist detection are considered to be effective countermeasures. The features of car-to-bicycle collisions need to be known in detail to develop such safety devices. The study investigated near-miss situations captured by drive recorders installed in passenger cars. Because similarities in the approach patterns between near-miss incidents and real-world fatal cyclist accidents in Japan were confirmed, we analyzed the 229 near-miss incident data via video capturing bicycles crossing the road in front of forward-moving cars. Using a video frame captured by a drive recorder, the time to collision (TTC) was calculated from the car's velocity and the distance between the car and bicycle at the moment when the bicycle initially appeared. The average TTC in the cases where bicycles emerged from behind obstructions was shorter than that in the cases where drivers had unobstructed views of the bicycles. In comparing the TTC of car-to-bicycle near-miss incidents to the previously obtained results of car-to-pedestrian near-miss incidents, it was determined that the average TTC in car-to-bicycle near-miss incidents was significantly longer than that in car-to-pedestrian near-miss incidents. When considering the TTC in the test protocol of evaluation for safety performance of active safety devices, we propose individual TTCs for evaluation of cyclist and pedestrian detections, respectively. In the test protocols, the following 2 scenarios should be employed: bicycle emerging from behind an unobstructed view and bicycle emerging from behind obstructions.

49 CFR 179.100-18 - Tests of tanks.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.100-18 Tests of tanks. (a) Each tank...

49 CFR 179.100-18 - Tests of tanks.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.100-18 Tests of tanks. (a) Each tank...

49 CFR 179.100-18 - Tests of tanks.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.100-18 Tests of tanks. (a) Each tank...

Passenger rail impact test. Volume 2 : summary of occupant protection program

DOT National Transportation Integrated Search

2003-07-01

On January 31, 2002, a train-to-train collision test was conducted involving a cab-car-led consist with three coach cars, all of conventional design, and a trailing locomotive traveling at 30 mph into a stationary locomotive coupled with two ballaste...

Rage against the machine? Google's self-driving cars versus human drivers.

PubMed

Teoh, Eric R; Kidd, David G

2017-12-01

Automated driving represents both challenges and opportunities in highway safety. Google has been developing self-driving cars and testing them under employee supervision on public roads since 2009. These vehicles have been involved in several crashes, and it is of interest how this testing program compares to human drivers in terms of safety. Google car crashes were coded by type and severity based on narratives released by Google. Crash rates per million vehicle miles traveled (VMT) were computed for crashes deemed severe enough to be reportable to police. These were compared with police-reported crash rates for human drivers. Crash types also were compared. Google cars had a much lower rate of police-reportable crashes per million VMT than human drivers in Mountain View, Calif., during 2009-2015 (2.19 vs 6.06), but the difference was not statistically significant. The most common type of collision involving Google cars was when they got rear-ended by another (human-driven) vehicle. Google cars shared responsibility for only one crash. These results suggest Google self-driving cars, while a test program, are safer than conventional human-driven passenger vehicles; however, currently there is insufficient information to fully examine the extent to which disengagements affected these results. Results suggest that highly-automated vehicles can perform more safely than human drivers in certain conditions, but will continue to be involved in crashes with conventionally-driven vehicles. Copyright © 2017. Published by Elsevier Ltd.

Small passenger car transmission test; Chevrolet LUV transmission

NASA Technical Reports Server (NTRS)

Bujold, M. P.

1980-01-01

A 1978 Chevrolet LUV manual transmission tested per the applicable portions of a passenger car automatic transmission test code (SAE J65lb) which required drive performance, coast performance, and no load test conditions. Under these test conditions, the transmission attained maximum efficiencies in the upper ninety percent range for both drive performance tests and coast performance tests. The major results of this test (torque, speed, and efficiency curves) are presented. Graphs map the complete performance characteristics for the Chevrolet LUV transmission.

Small passenger car transmission test; Ford C4 transmission

NASA Technical Reports Server (NTRS)

Bujold, M. P.

1980-01-01

A 1979 Ford C4 automatic transmission was tested per a passenger car automatic transmission test code (SAE J651b) which required drive performance, coast performance, and no load test conditions. Under these test conditions, the transmission attained maximum efficiencies in the mid-eighty percent range for both drive performance tests and coast performance tests. The major results of this test (torque, speed, and efficiency curves) are presented. Graphs map the complete performance characteristics for the Ford C4 transmission.

Parent versus child reporting of tobacco smoke exposure at home and in the car.

PubMed

Glover, Marewa; Hadwen, Georgy; Chelimo, Carol; Scragg, Robert; Bullen, Chris; Gentles, Dudley; Nosa, Vili; McCool, Judith

2013-05-31

To compare self-reported exposure to tobacco smoke in the home or in cars between parents and their pre-adolescent children. We analysed data on self-reported exposure to secondhand smoke from 3,645 matched pairs of children at baseline (aged between 10 and 13 years) and their parents whether smokers or not, who were participants in Keeping Kids Smokefree (KKS), a community-based study in South Auckland, New Zealand from 2007-2009. The study aimed to reduce children's smoking initiation through parental behaviour change. The responses of the parent-child pairs were analysed using proportions, Kappa scores, and McNemar's Chi-squared test. Additionally, 679 children were biochemically tested for smoking exposure using exhaled carbon monoxide. There was approximately a 30% discordance between the self-reports of children and their parents, with parents reporting less smoking in homes or cars than their children. Kappa scores for parent-child agreement by ethnicity ranged from 0.15 to 0.41 for smoking at home and 0.17 to 0.54 for smoking in cars. Biochemical testing suggested that around 30% of children had been exposed to secondhand smoke, corroborating their self-reported proportion of 37% (baseline in the home) whereas few parents (11%) reported smoking in home or cars. Parents were significantly less likely than children to report smoking inside the home or car. Biochemical testing indicated that children's reporting is more accurate. This has implications for future studies relying on self-reporting by children and/or their caregivers.

KSC-2012-3038a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train passes in front of the 525-foot-tall Vehicle Assembly Building, left, and the twin bays of the Orbiter Processing Facility, right, at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Low conspicuity of motorcycles for car drivers: dominant role of bottom-up control of visual attention or deficit of top-down control?

PubMed

Rogé, Joceline; Douissembekov, Evgueni; Vienne, Fabrice

2012-02-01

The aim of this study was to evaluate whether the low visibility of motorcycles is the result of their low cognitive conspicuity and/or their low sensory conspicuity for car drivers. In several cases of collision between a car and a motorcycle, the car driver failed to detect the motorcyclist in time to avoid the collision. To test the low cognitive conspicuity hypothesis, 42 car drivers (32.02 years old) including 21 motorcyclist motorists and 21 non-motorcyclist motorists carried out a motorcycle detection task in a car-driving simulator.To test the low sensory conspicuity hypothesis, the authors studied the effect of the color contrast between motorcycles and the road surface on the ability of car drivers to detect motorcycles when they appear from different parts of the road. A high level of color contrast enhanced the visibility of motorcycles when they appeared in front of the participants. Moreover, when motorcyclists appeared from behind the participants,the motorcyclist motorists detected oncoming motorcycles at a greater distance than did the non-motorcyclist motorists. Motorcyclist motorists carry out more saccades and rapidly capture information (on their rearview mirrors and on the road in front of them). The results related to the sensory conspicuity and cognitive conspicuity of motorcycles for car drivers are discussed from the viewpoint of visual attention theories. The practical implications of these results and future lines of research related to training methods for car drivers are considered.

KSC-2012-3046

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A manatee relaxes in the Indian River near the NASA Railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The manatee was spotted by a cameraman on hand to photograph a NASA Railroad train on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3044

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A manatee relaxes in the Indian River near the NASA Railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The manatee was spotted by a cameraman on hand to photograph a NASA Railroad train on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3037a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A NASA Railroad train passes in front of the 525-foot-tall Vehicle Assembly Building, left, and the twin bays of the Orbiter Processing Facility, right, at NASA’s Kennedy Space Center in Florida. The train is on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3043

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A manatee swims in the Indian River near the NASA Railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The manatee was spotted by a cameraman on hand to photograph a NASA Railroad train on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3045

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – Manatees relax in the Indian River near the NASA Railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The manatees were spotted by a cameraman on hand to photograph a NASA Railroad train on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3040a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – Bubbles form around a dolphin splashing in the Indian River near the NASA Railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The dolphin was spotted by a cameraman on hand to photograph a NASA Railroad train on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3041a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A dolphin plays in the Indian River near the NASA Railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The dolphin was spotted by a cameraman on hand to photograph a NASA Railroad train on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3042

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – A dolphin swims in the Indian River near the NASA Railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida. The dolphin was spotted by a cameraman on hand to photograph a NASA Railroad train on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Parkinson's disease and driving ability

PubMed Central

Singh, Rajiv; Pentland, Brian; Hunter, John; Provan, Frances

2007-01-01

Objectives To explore the driving problems associated with Parkinson's disease (PD) and to ascertain whether any clinical features or tests predict driver safety. Methods The driving ability of 154 individuals with PD referred to a driving assessment centre was determined by a combination of clinical tests, reaction times on a test rig and an in‐car driving test. Results The majority of cases (104, 66%) were able to continue driving although 46 individuals required an automatic transmission and 10 others needed car modifications. Ability to drive was predicted by the severity of physical disease, age, presence of other associated medical conditions, particularly dementia, duration of disease, brake reaction, time on a test rig and score on a driving test (all p<0.001). The level of drug treatment and the length of driving history were not correlated. Discriminant analysis revealed that the most important features in distinguishing safety to drive were severe physical disease (Hoehn and Yahr stage 3), reaction time, moderate disease associated with another medical condition and high score on car testing. Conclusions Most individuals with PD are safe to drive, although many benefit from car modifications or from using an automatic transmission. A combination of clinical tests and in‐car driving assessment will establish safety to drive, and a number of clinical correlates can be shown to predict the likely outcome and may assist in the decision process. This is the largest series of consecutive patients seen at a driving assessment centre reported to date, and the first to devise a scoring system for on‐road driving assessment. PMID:17178820

Deviation of the neurosporaxanthin pathway towards beta-carotene biosynthesis in Fusarium fujikuroi by a point mutation in the phytoene desaturase gene.

PubMed

Prado-Cabrero, Alfonso; Schaub, Patrick; Díaz-Sánchez, Violeta; Estrada, Alejandro F; Al-Babili, Salim; Avalos, Javier

2009-08-01

Carotenoids are widespread terpenoid pigments with applications in the food and feed industries. Upon illumination, the gibberellin-producing fungus Fusarium fujikuroi (Gibberella fujikuroi mating population C) develops an orange pigmentation caused by an accumulation of the carboxylic apocarotenoid neurosporaxanthin. The synthesis of this xanthophyll includes five desaturation steps presumed to be catalysed by the carB-encoded phytoene desaturase. In this study, we identified a yellow mutant (SF21) by mutagenesis of a carotenoid-overproducing strain. HPLC analyses indicated a specific impairment in the ability of SF21-CarB to perform the fifth desaturation, as implied by the accumulation of gamma-carotene and beta-carotene, which arise through four-step desaturation. Sequencing of the SF21 carB allele revealed a single mutation resulting in an exchange of a residue conserved in other five-step desaturases. Targeted carB allele replacement proved that this single mutation is the cause of the SF21 carotenoid pattern. In support, expression of SF21 CarB in engineered carotene-producing Escherichia coli strains demonstrated its reduced ability to catalyse the fifth desaturation step on both monocyclic and acyclic substrates. Further mutagenesis of SF21 led to the isolation of two mutants, SF73 and SF98, showing low desaturase activities, which mediated only two desaturation steps, resulting in accumulation of the intermediate zeta-carotene at low levels. Both strains contained an additional mutation affecting a CarB domain tentatively associated with carotenoid binding. SF21 exhibited higher carotenoid amounts than its precursor strain or the SF73 and SF98 mutants, although carotenogenic mRNA levels were similar in the four strains.

49 CFR 179.220-23 - Test of tanks.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.220-23 Test of tanks. (a) Each inner container...

Small passenger car transmission test: Mercury Lynx ATX transmission

NASA Technical Reports Server (NTRS)

Bujold, M. P.

1981-01-01

The testing of a Mercury Lynx automatic transmission is reported. The transmission was tested in accordance with a passenger car automatic transmission test code (SAE J65lb) which required drive performance, coast performance, and no load test conditions. Under these conditions, the transmission attained maximum efficiencies in the mid-ninety percent range both for drive performance test and coast performance tests. The torque, speed, and efficiency curves are presented, which provide the complete performance characteristics for the Mercury Lynx automatic transmission.

Polymorphisms in CARS are associated with gastric cancer risk: a two-stage case-control study in the Chinese population.

PubMed

Tian, Tian; Xiao, Ling; Du, Jiangbo; Zhu, Xun; Gu, Yayun; Qin, Na; Yan, Caiwang; Liu, Li; Ma, Hongxia; Jiang, Yue; Chen, Jiaping; Yu, Hao; Dai, Juncheng

2017-11-01

The cysteinyl transfer RNA synthetase gene (CARS) is located on chromosome band 11p15.5, which is an important tumor-suppressor gene region. Mutations in CARS have been identified in many kinds of cancers; however, evidence for a relationship between genetic variants in CARS and gastric cancer at the population level is still lacking. Thus, we explored the association of variants in CARS with gastric cancer using a two-stage case-control strategy in Chinese. We undertook a two-stage case-control study to investigate the association between polymorphisms in CARS and risk of gastric cancer with use of an Illumina Infinium ® BeadChip and an ABI 7900 system. Four single nucleotide polymorphisms (SNPs) were significantly associated with gastric cancer risk in both the discovery stage and the validation stage after adjustment for age and sex. In addition, the combined results of the two stages showed these SNPs were related to gastric cancer risk (P false discovery rate  ≤ 0.001 for rs384,490, rs729662, rs2071101, and rs7394702). In silico analyses revealed that rs384490 and rs7394702 could affect transcription factor response elements or DNA methylation of CARS, and rs729662 was associated with the prognosis of gastric cancer. Additionally, expression quantitative trait loci analysis showed rs384490 and rs729662 might alter expression of CARS-related genes. The potential functional SNPs in CARS might influence the biological functions of CARS or CARS-related genes and ultimately modify the occurrence and development of gastric cancer in Chinese. Further large-scale population-based studies or biological functional assays are warranted to validate our findings.

Modeling of Aerodynamic Force Acting in Tunnel for Analysis of Riding Comfort in a Train

NASA Astrophysics Data System (ADS)

Kikko, Satoshi; Tanifuji, Katsuya; Sakanoue, Kei; Nanba, Kouichiro

In this paper, we aimed to model the aerodynamic force that acts on a train running at high speed in a tunnel. An analytical model of the aerodynamic force is developed from pressure data measured on car-body sides of a test train running at the maximum revenue operation speed. The simulation of an 8-car train running while being subjected to the modeled aerodynamic force gives the following results. The simulated car-body vibration corresponds to the actual vibration both qualitatively and quantitatively for the cars at the rear of the train. The separation of the airflow at the tail-end of the train increases the yawing vibration of the tail-end car while it has little effect on the car-body vibration of the adjoining car. Also, the effect of the moving velocity of the aerodynamic force on the car-body vibration is clarified that the simulation under the assumption of a stationary aerodynamic force can markedly increase the car-body vibration.

78 FR 2236 - Federal Motor Vehicle Safety Standards; New Pneumatic Tires for Motor Vehicles With a GVWR of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-10

... Society of Automotive Engineers (SAE) Recommended Practice J918b--Passenger Car Tire Performance Requirements and Test Procedures (January 1967). 2, 3 As part of the strength test, a plunger is driven into a...--Passenger Car Tire Performance Requirements and Test Procedures (January 1967) Section 3.1. \\5\\ FMVSS No...

An investigation into the factors that influence toolmark identifications on ammunition discharged from semi-automatic pistols recovered from car fires.

PubMed

Collender, Mark A; Doherty, Kevin A J; Stanton, Kenneth T

2017-01-01

Following a shooting incident where a vehicle is used to convey the culprits to and from the scene, both the getaway car and the firearm are often deliberately burned in an attempt to destroy any forensic evidence which may be subsequently recovered. Here we investigate the factors that influence the ability to make toolmark identifications on ammunition discharged from pistols recovered from such car fires. This work was carried out by conducting a number of controlled furnace tests in conjunction with real car fire tests in which three 9mm semi-automatic pistols were burned. Comparisons between pre-burn and post burn test fired ammunition discharged from these pistols were then performed to establish if identifications were still possible. The surfaces of the furnace heated samples and car fire samples were examined following heating/burning to establish what factors had influenced their surface morphology. The primary influence on the surfaces of the furnace heated and car fire samples was the formation of oxide layers. The car fire samples were altered to a greater extent than the furnace heated samples. Identifications were still possible between pre- and post-burn discharged cartridge cases, but this was not the case for the discharged bullets. It is suggested that the reason for this is a difference between the types of firearms discharge-generated toolmarks impressed onto the base of cartridge cases compared to those striated along the surfaces of bullets. It was also found that the temperatures recorded in the front foot wells were considerably less than those recorded on top of the rear seats during the car fires. These factors should be assessed by forensic firearms examiners when performing casework involving pistols recovered from car fires. Copyright © 2016 The Chartered Society of Forensic Sciences. Published by Elsevier Ireland Ltd. All rights reserved.

The cortisol awakening response and memory performance in older men and women.

PubMed

Almela, Mercedes; van der Meij, Leander; Hidalgo, Vanesa; Villada, Carolina; Salvador, Alicia

2012-12-01

The activity and regulation of the hypothalamus-pituitary-adrenal axis has been related to cognitive decline during aging. This study investigated whether the cortisol awakening response (CAR) is related to memory performance among older adults. The sample was composed of 88 participants (44 men and 44 women) from 55 to 77 years old. The memory assessment consisted of two tests measuring declarative memory (a paragraph recall test and a word list learning test) and two tests measuring working memory (a spatial span test and a spatial working memory test). Among those participants who showed the CAR on two consecutive days, we found that a greater CAR was related to poorer declarative memory performance in both men and women, and to better working memory performance only in men. The results of our study suggest that the relationship between CAR and memory performance is negative in men and women when memory performance is largely dependent on hippocampal functioning (i.e. declarative memory), and positive, but only in men, when memory performance is largely dependent on prefrontal cortex functioning (i.e. working memory). Copyright © 2012 Elsevier Ltd. All rights reserved.

Response Surface Methods for Spatially-Resolved Optical Measurement Techniques

NASA Technical Reports Server (NTRS)

Danehy, P. M.; Dorrington, A. A.; Cutler, A. D.; DeLoach, R.

2003-01-01

Response surface methods (or methodology), RSM, have been applied to improve data quality for two vastly different spatial ly-re solved optical measurement techniques. In the first application, modern design of experiments (MDOE) methods, including RSM, are employed to map the temperature field in a direct-connect supersonic combustion test facility at NASA Langley Research Center. The laser-based measurement technique known as coherent anti-Stokes Raman spectroscopy (CARS) is used to measure temperature at various locations in the combustor. RSM is then used to develop temperature maps of the flow. Even though the temperature fluctuations at a single point in the flowfield have a standard deviation on the order of 300 K, RSM provides analytic fits to the data having 95% confidence interval half width uncertainties in the fit as low as +/-30 K. Methods of optimizing future CARS experiments are explored. The second application of RSM is to quantify the shape of a 5-meter diameter, ultra-light, inflatable space antenna at NASA Langley Research Center.

A spring-mass-damper system dynamics-based driver-vehicle integrated model for representing heterogeneous traffic

NASA Astrophysics Data System (ADS)

Munigety, Caleb Ronald

2018-04-01

The traditional traffic microscopic simulation models consider driver and vehicle as a single unit to represent the movements of drivers in a traffic stream. Due to this very fact, the traditional car-following models have the driver behavior related parameters, but ignore the vehicle related aspects. This approach is appropriate for homogeneous traffic conditions where car is the major vehicle type. However, in heterogeneous traffic conditions where multiple vehicle types are present, it becomes important to incorporate the vehicle related parameters exclusively to account for the varying dynamic and static characteristics. Thus, this paper presents a driver-vehicle integrated model hinged on the principles involved in physics-based spring-mass-damper mechanical system. While the spring constant represents the driver’s aggressiveness, the damping constant and the mass component take care of the stability and size/weight related aspects, respectively. The proposed model when tested, behaved pragmatically in representing the vehicle-type dependent longitudinal movements of vehicles.

The performance of an automatic acoustic-based program classifier compared to hearing aid users' manual selection of listening programs.

PubMed

Searchfield, Grant D; Linford, Tania; Kobayashi, Kei; Crowhen, David; Latzel, Matthias

2018-03-01

To compare preference for and performance of manually selected programmes to an automatic sound classifier, the Phonak AutoSense OS. A single blind repeated measures study. Participants were fit with Phonak Virto V90 ITE aids; preferences for different listening programmes were compared across four different sound scenarios (speech in: quiet, noise, loud noise and a car). Following a 4-week trial preferences were reassessed and the users preferred programme was compared to the automatic classifier for sound quality and hearing in noise (HINT test) using a 12 loudspeaker array. Twenty-five participants with symmetrical moderate-severe sensorineural hearing loss. Participant preferences of manual programme for scenarios varied considerably between and within sessions. A HINT Speech Reception Threshold (SRT) advantage was observed for the automatic classifier over participant's manual selection for speech in quiet, loud noise and car noise. Sound quality ratings were similar for both manual and automatic selections. The use of a sound classifier is a viable alternative to manual programme selection.

1-kHz two-dimensional coherent anti-Stokes Raman scattering (2D-CARS) for gas-phase thermometry.

PubMed

Miller, Joseph D; Slipchenko, Mikhail N; Mance, Jason G; Roy, Sukesh; Gord, James R

2016-10-31

Two-dimensional gas-phase coherent anti-Stokes Raman scattering (2D-CARS) thermometry is demonstrated at 1 kHz in a heated jet. A hybrid femtosecond/picosecond CARS configuration is used in a two-beam phase-matching arrangement with a 100-femtosecond pump/Stokes pulse and a 107-picosecond probe pulse. The femtosecond pulse is generated using a mode-locked oscillator and regenerative amplifier that is synchronized to a separate picosecond oscillator and burst-mode amplifier. The CARS signal is spectrally dispersed in a custom imaging spectrometer and detected using a high-speed camera with image intensifier. 1-kHz, single-shot planar measurements at room temperature exhibit error of 2.6% and shot-to-shot variations of 2.6%. The spatial variation in measured temperature is 9.4%. 2D-CARS temperature measurements are demonstrated in a heated O₂ jet to capture the spatiotemporal evolution of the temperature field.

Design and modeling of a prototype fiber scanning CARS endoscope

NASA Astrophysics Data System (ADS)

Veilleux, Isra"l.; Doucet, Michel; Coté, Patrice; Verreault, Sonia; Fortin, Michel; Paradis, Patrick; Leclair, Sébastien; Da Costa, Ralph S.; Wilson, Brian C.; Seibel, Eric; Mermut, Ozzy; Cormier, Jean-François

2010-02-01

An endoscope capable of Coherent Anti-Stokes Raman scattering (CARS) imaging would be of significant clinical value for improving early detection of endoluminal cancers. However, developing this technology is challenging for many reasons. First, nonlinear imaging techniques such as CARS are single point measurements thus requiring fast scanning in a small footprint if video rate is to be achieved. Moreover, the intrinsic nonlinearity of this modality imposes several technical constraints and limitations, mainly related to pulse and beam distortions that occur within the optical fiber and the focusing objective. Here, we describe the design and report modeling results of a new CARS endoscope. The miniature microscope objective design and its anticipated performance are presented, along with its compatibility with a new spiral scanningfiber imaging technology developed at the University of Washington. This technology has ideal attributes for clinical use, with its small footprint, adjustable field-of-view and high spatial-resolution. This compact hybrid fiber-based endoscopic CARS imaging design is anticipated to have a wide clinical applicability.

Statistical analyses of influence of solar and geomagnetic activities on car accident events

NASA Astrophysics Data System (ADS)

Alania, M. V.; Gil, A.; Wieliczuk, R.

2001-01-01

Statistical analyses of the influence of Solar and geomagnetic activity, sector structure of the interplanetary magnetic field and galactic cosmic ray Forbush effects on car accident events in Poland for the period of 1990-1999 have been carried out. Using auto-correlation, cross-correlation, spectral analyses and superposition epochs methods it has been shown that there are separate periods when car accident events have direct correlation with Ap index of the geomagnetic activity, sector structure of the interplanetary magnetic field and Forbush decreases of galactic cosmic rays. Nevertheless, the single-valued direct correlation is not possible to reveal for the whole period of 1990-1999. Periodicity of 7 days and its second harmonic (3.5 days) has been reliably revealed in the car accident events data in Poland for the each year of the period 1990-1999. It is shown that the maximum car accident events take place in Poland on Friday and practically does not depend on the level of solar and geomagnetic activities.

Adenovirus tumor targeting and hepatic untargeting by a coxsackie/adenovirus receptor ectodomain anti-carcinoembryonic antigen bispecific adapter.

PubMed

Li, Hua-Jung; Everts, Maaike; Pereboeva, Larisa; Komarova, Svetlana; Idan, Anat; Curiel, David T; Herschman, Harvey R

2007-06-01

Adenovirus vectors have a number of advantages for gene therapy. However, because of their lack of tumor tropism and their preference for liver infection following systemic administration, they cannot be used for systemic attack on metastatic disease. Many epithelial tumors (e.g., colon, lung, and breast) express carcinoembryonic antigen (CEA). To block the natural hepatic tropism of adenovirus and to "retarget" the virus to CEA-expressing tumors, we used a bispecific adapter protein (sCAR-MFE), which fuses the ectodomain of the coxsackie/adenovirus receptor (sCAR) with a single-chain anti-CEA antibody (MFE-23). sCAR-MFE untargets adenovirus-directed luciferase transgene expression in the liver by >90% following systemic vector administration. Moreover, sCAR-MFE can "retarget" adenovirus to CEA-positive epithelial tumor cells in cell culture, in s.c. tumor grafts, and in hepatic tumor grafts. The sCAR-MFE bispecific adapter should, therefore, be a powerful agent to retarget adenovirus vectors to epithelial tumor metastases.

49 CFR 179.100-19 - Tests of safety relief valves.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Tests of safety relief valves. 179.100-19 Section... MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120...

49 CFR 179.100-19 - Tests of safety relief valves.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 3 2011-10-01 2011-10-01 false Tests of safety relief valves. 179.100-19 Section... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.100-19...

49 CFR 179.100-19 - Tests of safety relief valves.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 3 2014-10-01 2014-10-01 false Tests of safety relief valves. 179.100-19 Section... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.100-19...

49 CFR 179.100-19 - Tests of safety relief valves.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Tests of safety relief valves. 179.100-19 Section... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specifications for Pressure Tank Car Tanks (Classes DOT-105, 109, 112, 114 and 120) § 179.100-19...

Intelligent cruise control field operational test. Vol III, Performance of a string or cluster of ACC-equipped cars

DOT National Transportation Integrated Search

1998-07-01

This report is one element of a cooperative agreement between NHTSA and UMTRI entitled Intelligent Cruise Control (ICC) Field Operational Test (FOT). It addresses the operation of a serial string or dense cluster of passenger cars equipped with a new...

Hybrid single-source online Fourier transform coherent anti-Stokes Raman scattering/optical coherence tomography.

PubMed

Kamali, Tschackad; Považay, Boris; Kumar, Sunil; Silberberg, Yaron; Hermann, Boris; Werkmeister, René; Drexler, Wolfgang; Unterhuber, Angelika

2014-10-01

We demonstrate a multimodal optical coherence tomography (OCT) and online Fourier transform coherent anti-Stokes Raman scattering (FTCARS) platform using a single sub-12 femtosecond (fs) Ti:sapphire laser enabling simultaneous extraction of structural and chemical ("morphomolecular") information of biological samples. Spectral domain OCT prescreens the specimen providing a fast ultrahigh (4×12  μm axial and transverse) resolution wide field morphologic overview. Additional complementary intrinsic molecular information is obtained by zooming into regions of interest for fast label-free chemical mapping with online FTCARS spectroscopy. Background-free CARS is based on a Michelson interferometer in combination with a highly linear piezo stage, which allows for quick point-to-point extraction of CARS spectra in the fingerprint region in less than 125 ms with a resolution better than 4  cm(-1) without the need for averaging. OCT morphology and CARS spectral maps indicating phosphate and carbonate bond vibrations from human bone samples are extracted to demonstrate the performance of this hybrid imaging platform.

Dual CARS and SHG image acquisition scheme that combines single central fiber and multimode fiber bundle to collect and differentiate backward and forward generated photons

PubMed Central

Weng, Sheng; Chen, Xu; Xu, Xiaoyun; Wong, Kelvin K.; Wong, Stephen T. C.

2016-01-01

In coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) imaging, backward and forward generated photons exhibit different image patterns and thus capture salient intrinsic information of tissues from different perspectives. However, they are often mixed in collection using traditional image acquisition methods and thus are hard to interpret. We developed a multimodal scheme using a single central fiber and multimode fiber bundle to simultaneously collect and differentiate images formed by these two types of photons and evaluated the scheme in an endomicroscopy prototype. The ratio of these photons collected was calculated for the characterization of tissue regions with strong or weak epi-photon generation while different image patterns of these photons at different tissue depths were revealed. This scheme provides a new approach to extract and integrate information captured by backward and forward generated photons in dual CARS/SHG imaging synergistically for biomedical applications. PMID:27375938

KSC-2012-3049

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – NASA Railroad locomotive No. 3 delivers tank cars from NASA’s Kennedy Space Center in Florida to the Florida East Coast Railway interchange in Titusville, Fla. The locomotive is one of three NASA Railroad locomotives built for the Toledo, Peoria and Western, or TP&W, between 1968 and 1970. It is a GM Electromotive Division SW-1500 switcher. The locomotive was acquired by NASA from the TP&W in 1984 and painted in the NASA Railroad paint scheme. The power plant was completely overhauled in 2009. The locomotive will pull the train to the interchange in Titusville, where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3031a

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – NASA Railroad locomotive No. 3 is enlisted to deliver tank cars from NASA’s Kennedy Space Center in Florida to the Florida East Coast Railway interchange in Titusville, Fla. The locomotive is one of three NASA Railroad locomotives built for the Toledo, Peoria and Western, or TP&W, between 1968 and 1970. It is a GM Electromotive Division SW-1500 switcher. The locomotive was acquired by NASA from the TP&W in 1984 and painted in the NASA Railroad paint scheme. The power plant was completely overhauled in 2009. The locomotive will pull the train to the interchange in Titusville, where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-3048

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – NASA Railroad locomotive No. 3 delivers tank cars from NASA’s Kennedy Space Center in Florida to the Florida East Coast Railway interchange in Titusville, Fla. The locomotive is one of three NASA Railroad locomotives built for the Toledo, Peoria and Western, or TP&W, between 1968 and 1970. It is a GM Electromotive Division SW-1500 switcher. The locomotive was acquired by NASA from the TP&W in 1984 and painted in the NASA Railroad paint scheme. The power plant was completely overhauled in 2009. The locomotive will pull the train to the interchange in Titusville, where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Benefits of magnesium wheels for consumer cars

NASA Astrophysics Data System (ADS)

Frishfelds, Vilnis; Timuhins, Andrejs; Bethers, Uldis

2018-05-01

Advantages and disadvantages of magnesium wheels are considered based on a mechanical model of a car. Magnesium wheels are usually applied to racing cars as they provide slightly better strength/weight ratio than aluminum alloys. Do they provide notable benefits also for the everyday user when the car speeds do not exceed allowed speed limit? Distinct properties of magnesium rims are discussed. Apart from lighter weight of magnesium alloys, they are also good in dissipating the energy of vibrations. The role of energy dissipation in the rim of a wheel is estimated by a quarter car model. Improvements to safety by using the magnesium wheels are considered. Braking distance and responsiveness of the car is studied both with and without using an Anti Blocking System (ABS). Influence of rim weight on various handling parameters of the car is quantitatively tested.

Advances in engineering of high contrast CARS imaging endoscopes

PubMed Central

Deladurantaye, Pascal; Paquet, Alex; Paré, Claude; Zheng, Huimin; Doucet, Michel; Gay, David; Poirier, Michel; Cormier, Jean-François; Mermut, Ozzy; Wilson, Brian C.; Seibel, Eric J.

2014-01-01

The translation of CARS imaging towards real time, high resolution, chemically selective endoscopic tissue imaging applications is limited by a lack of sensitivity in CARS scanning probes sufficiently small for incorporation into endoscopes. We have developed here a custom double clad fiber (DCF)-based CARS probe which is designed to suppress the contaminant Four-Wave-Mixing (FWM) background generated within the fiber and integrated it into a fiber based scanning probe head of a few millimeters in diameter. The DCF includes a large mode area (LMA) core as a first means of reducing FWM generation by ~3 dB compared to commercially available, step-index single mode fibers. A micro-fabricated miniature optical filter (MOF) was grown on the distal end of the DCF to block the remaining FWM background from reaching the sample. The resulting probe was used to demonstrate high contrast images of polystyrene beads in the forward-CARS configuration with > 10 dB suppression of the FWM background. In epi-CARS geometry, images exhibited lower contrast due to the leakage of MOF-reflected FWM from the fiber core. Improvements concepts for the fiber probe are proposed for high contrast epi-CARS imaging to enable endoscopic implementation in clinical tissue assessment contexts, particularly in the early detection of endoluminal cancers and in tumor margin assessment. PMID:25401538

Hybrid fs/ps CARS for Sooting and Particle-laden Flames

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoffmeister, Kathryn N. Gabet; Guildenbecher, Daniel Robert; Kearney, Sean P.

2015-12-01

We report the application of ultrafast rotational coherent anti-Stokes Raman scattering (CARS) for temperature and relative oxygen concentration measurements in the plume emanating from a burning aluminized ammonium perchlorate propellant strand. Combustion of these metal-based propellants is a particularly hostile environment for laserbased diagnostics, with intense background luminosity, scattering and beam obstruction from hot metal particles that can be as large as several hundred microns in diameter. CARS spectra that were previously obtained using nanosecond pulsed lasers in an aluminumparticle- seeded flame are examined and are determined to be severely impacted by nonresonant background, presumably as a result of themore » plasma formed by particulateenhanced laser-induced breakdown. Introduction of fs/ps laser pulses enables CARS detection at reduced pulse energies, decreasing the likelihood of breakdown, while simultaneously providing time-gated elimination of any nonresonant background interference. Temperature probability densities and temperature/oxygen correlations were constructed from ensembles of several thousand single-laser-shot measurements from the fs/ps rotational CARS measurement volume positioned within 3 mm or less of the burning propellant surface. Preliminary results in canonical flames are presented using a hybrid fs/ps vibrational CARS system to demonstrate our progress towards acquiring vibrational CARS measurements for more accurate temperatures in the very high temperature propellant burns.« less

A GMP-compliant protocol to expand and transfect cancer patient T cells with mRNA encoding a tumor-specific chimeric antigen receptor.

PubMed

Krug, Christian; Wiesinger, Manuel; Abken, Hinrich; Schuler-Thurner, Beatrice; Schuler, Gerold; Dörrie, Jan; Schaft, Niels

2014-10-01

Chimeric antigen receptors (CARs), which combine an antibody-derived binding domain (single chain fragment variable) with T-cell-activating signaling domains, have become a promising tool in the adoptive cellular therapy of cancer. Retro- and lenti-viral transductions are currently the standard methods to equip T cells with a CAR; permanent CAR expression, however, harbors several risks like uncontrolled auto-reactivity. Modification of T cells by electroporation with CAR-encoding RNA to achieve transient expression likely circumvents these difficulties. We here present a GMP-compliant protocol to activate and expand T cells for clinical application. The protocol is optimized in particular to produce CAR-modified T cells in clinically sufficient numbers under full GMP-compliance from late-stage cancer patients. This protocol allows the generation of 6.7 × 10(8) CAR-expressing T cells from one patient leukapheresis. The CAR-engineered T cells produced pro-inflammatory cytokines after stimulation with antigen-bearing tumor cells and lysed tumor cells in an antigen-specific manner. This functional capacity was maintained after cryopreservation. Taken together, we provide a clinically applicable protocol to transiently engineer sufficient numbers of antigen-specific patient T cells for use in adoptive cell therapy of cancer.

A BLE-Based Pedestrian Navigation System for Car Searching in Indoor Parking Garages

PubMed Central

Wang, Sheng-Shih

2018-01-01

The continuous global increase in the number of cars has led to an increase in parking issues, particularly with respect to the search for available parking spaces and finding cars. In this paper, we propose a navigation system for car owners to find their cars in indoor parking garages. The proposed system comprises a car-searching mobile app and a positioning-assisting subsystem. The app guides car owners to their cars based on a “turn-by-turn” navigation strategy, and has the ability to correct the user’s heading orientation. The subsystem uses beacon technology for indoor positioning, supporting self-guidance of the car-searching mobile app. This study also designed a local coordinate system to support the identification of the locations of parking spaces and beacon devices. We used Android as the platform to implement the proposed car-searching mobile app, and used Bytereal HiBeacon devices to implement the proposed positioning-assisting subsystem. We also deployed the system in a parking lot in our campus for testing. The experimental results verified that the proposed system not only works well, but also provides the car owner with the correct route guidance information. PMID:29734753

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pearce, T.C.; Waters, M.H.L.

Measurements have been made of the fuel consumption of a petrol and a diesel car when starting from cold. The cars were the 1.1 liter petrol VW Golf and the 1.5 liter diesel version, which have the same passenger accommodation and nearly identical road performance. It was found that the diesel car used less fuel in the warm-up period than the petrol, both when being driven at constant speed on a test track and with the engine idling and the car stationary. (Copyright (c) Crown Copyright 1980.)

Feasibility of controlling CD38-CAR T cell activity with a Tet-on inducible CAR design

PubMed Central

Poels, Renée; Mulders, Manon J.; van de Donk, Niels W. C. J.; Themeli, Maria; Lokhorst, Henk M.; Mutis, Tuna

2018-01-01

Recent clinical advances with chimeric antigen receptor (CAR) T cells have led to the accelerated clinical approval of CD19-CARs to treat acute lymphoblastic leukemia. The CAR T cell therapy is nevertheless associated with toxicities, especially if the CARs are not entirely tumor-specific. Therefore, strategies for controlling the CAR T cell activity are required to improve their safety profile. Here, by using the multiple myeloma (MM)-associated CD38 molecule as target molecule, we tested the feasibility and utility of a doxycycline (DOX) inducible Tet-on CD38-CAR design to control the off-target toxicities of CAR T cells. Using CARs with high affinity to CD38, we demonstrate that this strategy allows the proper induction of CD38-CARs and CAR-mediated T cell cytotoxicity in a DOX-dose dependent manner. Especially when the DOX dose was limited to 10ng/ml, its removal resulted in a relatively rapid decay of CAR- related off-tumor effects within 24 hours, indicating the active controllability of undesired CAR activity. This Tet-on CAR design also allowed us to induce the maximal anti-MM cytotoxic activity of affinity-optimized CD38-CAR T cells, which already display a low toxicity profile, hereby adding a second level of safety to these cells. Collectively, these results indicate the possibility to utilize this DOX inducible CAR-design to actively regulate the CAR-mediated activities of therapeutic T cells. We therefore conclude that the Tet-on system may be more advantageous above suicide-genes to control the potential toxicities of CAR T cells without the need to destroy them permanently. PMID:29847570

Chemical release from single-PMMA microparticles monitored by CARS microscopy

NASA Astrophysics Data System (ADS)

Enejder, Annika; Svedberg, Fredrik; Nordstierna, Lars; Nydén, Magnus

2011-03-01

Microparticles loaded with antigens, proteins, DNA, fungicides, and other functional agents emerge as ideal vehicles for vaccine, drug delivery, genetic therapy, surface- and crop protection. The microscopic size of the particles and their collective large specific surface area enables highly active and localized release of the functional substance. In order to develop designs with release profiles optimized for the specific application, it is desirable to map the distribution of the active substance within the particle and how parameters such as size, material and morphology affect release rates at single particle level. Current imaging techniques are limited in resolution, sensitivity, image acquisition time, or sample treatment, excluding dynamic studies of active agents in microparticles. Here, we demonstrate that the combination of CARS and THG microscopy can successfully be used, by mapping the spatial distribution and release rates of the fungicide and food preservative IPBC from different designs of PMMA microparticles at single-particle level. By fitting a radial diffusion model to the experimental data, single particle diffusion coefficients can be determined. We show that release rates are highly dependent on the size and morphology of the particles. Hence, CARS and THG microscopy provides adequate sensitivity and spatial resolution for quantitative studies on how singleparticle properties affect the diffusion of active agents at microscopic level. This will aid the design of innovative microencapsulating systems for controlled release.

The Effects of the Capital Accumulation Ratio on Wealth

ERIC Educational Resources Information Center

Harness, Nathaniel J.; Finke, Michael S.; Chatterjee, Swarn

2009-01-01

The capital accumulation ratio (CAR) is commonly used in academic research as a measure of household portfolio quality. This study tested whether a higher initial CAR impacts change in wealth over a decade among households in the accumulation life cycle stage. Meeting the 25% CAR guideline resulted in a 28.1% increase in net worth between 1994 and…

KSC-2012-3047

NASA Image and Video Library

2012-05-23

CAPE CANAVERAL, Fla. – Preparations are under way at the NASA Railroad’s Jay Jay Railroad Bridge north of Launch Complex 39 at NASA’s Kennedy Space Center in Florida for the passage of the NASA Railroad train on its way to the Florida East Coast Railway interchange in Titusville, Fla., where the train’s helium tank cars, a liquid oxygen tank car, and a liquid hydrogen dewar or tank car will be transferred for delivery to the SpaceX engine test complex outside McGregor, Texas. The permanent configuration of the drawbridge span is open, but the span will be lowered for a train to cross. The railroad cars were needed in support of the Space Shuttle Program but currently are not in use by NASA following the completion of the program in 2011. Originally, the tankers belonged to the U.S. Bureau of Mines. At the peak of the shuttle program, there were approximately 30 cars in the fleet. About half the cars were returned to the bureau as launch activity diminished. Five tank cars are being loaned to SpaceX and repurposed to support their engine tests in Texas. Eight cars previously were shipped to California on loan to support the SpaceX Falcon 9 rocket launches from Space Launch Complex-4 on Vandenberg Air Force Base. SpaceX already has three helium tank cars previously used for the shuttle program at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

Photocatalytic degradation of NOx gases using TiO2-containing paint: a real scale study.

PubMed

Maggos, Th; Bartzis, J G; Liakou, M; Gobin, C

2007-07-31

An indoor car park was appropriately equipped in order to test the de-polluting efficiency of a TiO(2)-containing paint in an indoor polluted environment, under real scale configuration. Depollution tests were performed in an artificially closed area of the parking, which was polluted by a car exhaust during the testing period. The ceiling surface of the car park was covered with white acrylic TiO(2)-containing paint (PP), which was developed in the frame of the EU project 'PICADA' (Photocatalytic Innovative Coverings Application for Depollution Assessment). The closed area was fed with car exhaust gases. As soon as the system reached steady state, the UV lamps were turned on for 5h. The difference between the final and the initial steady state concentration indicates the removal of the pollutants due to both the photocatalytic paint and car emission reduction. Results showed a significant photocatalytic oxidation of NO(x) gases. The photocatalytic removal of NO and NO(2) was calculated to 19% and 20%, respectively, while the photocatalytic rate (microgm(-2)s(-1)) ranged between 0.05 and 0.13 for NO and between 0.09 and 0.16 for NO(2).

Transgenic Expression of IL15 Improves Antiglioma Activity of IL13Rα2-CAR T Cells but Results in Antigen Loss Variants.

PubMed

Krenciute, Giedre; Prinzing, Brooke L; Yi, Zhongzhen; Wu, Meng-Fen; Liu, Hao; Dotti, Gianpietro; Balyasnikova, Irina V; Gottschalk, Stephen

2017-07-01

Glioblastoma (GBM) is the most aggressive primary brain tumor in adults and is virtually incurable with conventional therapies. Immunotherapy with T cells expressing GBM-specific chimeric antigen receptors (CAR) is an attractive approach to improve outcomes. Although CAR T cells targeting GBM antigens, such as IL13 receptor subunit α2 (IL13Rα2), HER2, and EGFR variant III (EGFRvIII), have had antitumor activity in preclinical models, early-phase clinical testing has demonstrated limited antiglioma activity. Transgenic expression of IL15 is an appealing strategy to enhance CAR T-cell effector function. We tested this approach in our IL13Rα2-positive glioma model in which limited IL13Rα2-CAR T-cell persistence results in recurrence of antigen-positive gliomas. T cells were genetically modified with retroviral vectors encoding IL13Rα2-CARs or IL15 (IL13Rα2-CAR.IL15 T cells). IL13Rα2-CAR.IL15 T cells recognized glioma cells in an antigen-dependent fashion, had greater proliferative capacity, and produced more cytokines after repeated stimulations in comparison with IL13Rα2-CAR T cells. No autonomous IL13Rα2-CAR.IL15 T-cell proliferation was observed; however, IL15 expression increased IL13Rα2-CAR T-cell viability in the absence of exogenous cytokines or antigen. In vivo , IL13Rα2-CAR.IL15 T cells persisted longer and had greater antiglioma activity than IL13Rα2-CAR T cells, resulting in a survival advantage. Gliomas recurring after 40 days after T-cell injection had downregulated IL13Rα2 expression, indicating that antigen loss variants occur in the setting of improved T-cell persistence. Thus, CAR T cells for GBM should not only be genetically modified to improve their proliferation and persistence, but also to target multiple antigens. Summary: Glioblastoma responds imperfectly to immunotherapy. Transgenic expression of IL15 in T cells expressing CARs improved their proliferative capacity, persistence, and cytokine production. The emergence of antigen loss variants highlights the need to target multiple tumor antigens. Cancer Immunol Res; 5(7); 571-81. ©2017 AACR . ©2017 American Association for Cancer Research.

Robust Multivariable Estimation of the Relevant Information Coming from a Wheel Speed Sensor and an Accelerometer Embedded in a Car under Performance Tests

PubMed Central

Hernandez, Wilmar

2005-01-01

In the present paper, in order to estimate the response of both a wheel speed sensor and an accelerometer placed in a car under performance tests, robust and optimal multivariable estimation techniques are used. In this case, the disturbances and noises corrupting the relevant information coming from the sensors' outputs are so dangerous that their negative influence on the electrical systems impoverish the general performance of the car. In short, the solution to this problem is a safety related problem that deserves our full attention. Therefore, in order to diminish the negative effects of the disturbances and noises on the car's electrical and electromechanical systems, an optimum observer is used. The experimental results show a satisfactory improvement in the signal-to-noise ratio of the relevant signals and demonstrate the importance of the fusion of several intelligent sensor design techniques when designing the intelligent sensors that today's cars need.

Coherent anti-Stokes Raman scattering for quantitative temperature and concentration measurements in a high-pressure gas turbine combustor rig

NASA Astrophysics Data System (ADS)

Thariyan, Mathew Paul

Dual-pump coherent anti-Stokes Raman scattering (DP-CARS) temperature and major species (CO2/N2) concentration measurements have been performed in an optically-accessible high-pressure gas turbine combustor facility (GTCF) and for partially-premixed and non-premixed flames in a laminar counter-flow burner. A window assembly incorporating pairs of thin and thick fused silica windows on three sides was designed, fabricated, and assembled in the GTCF for advanced laser diagnostic studies. An injection-seeded optical parametric oscillator (OPO) was used as a narrowband pump laser source in the dual-pump CARS system. Large prisms on computer-controlled translation stages were used to direct the CARS beams either into the main optics leg for measurements in the GTCF or to a reference optics leg for measurements of the nonresonant CARS spectrum and for aligning the CARS system. Combusting flows were stabilized with liquid fuel injection only for the central injector of a 9-element lean direct injection (LDI) device developed at NASA Glenn Research Center. The combustor was operated using Jet A fuel at inlet air temperatures up to 725 K and combustor pressures up to 1.03 MPa. Single-shot DP-CARS spectra were analyzed using the Sandia CARSFT code in the batch operation mode to yield instantaneous temperature and CO2/N2 concentration ratio values. Spatial maps of mean and standard deviations of temperature and CO2/N2 concentrations were obtained in the high-pressure LDI flames by translating the CARS probe volume in axial and vertical directions inside the combustor rig. The mean temperature fields demonstrate the effect of the combustor conditions on the overall flame length and the average flame structure. The temperature relative standard deviation values indicate thermal fluctuations due to the presence of recirculation zones and/or flame brush fluctuations. The correlation between the temperature and relative CO 2 concentration data has been studied at various combustor conditions. The insight into the reacting flow structure provided by these measurements is discussed. Such measurements at conditions similar to those of aircraft gas turbine combustors are extremely useful for testing combustion models being used to predict performance of these systems.

Hybrid Electric Vehicle, Winner of the "FutureCar Challenge

Science.gov Websites

more information contact: George Douglas (303) 275-4096 or (303) 880-2913 (cellular) Golden, Colo at NREL, the car will recharge its batteries at the lab's photovoltaic Outdoor Test Facility, where to the laboratory. Where: NREL's Outdoor Test Facility. Check in at the Visitor's Center to be

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoffmeister, Kathryn N. Gabet; Guildenbecher, Daniel Robert; Kearney, Sean P.

We report the application of ultrafast rotational coherent anti-Stokes Raman scattering (CARS) for temperature and relative oxygen concentration measurements in the plume emanating from a burning aluminized ammonium perchlorate propellant strand. Combustion of these metal-based propellants is a particularly hostile environment for laserbased diagnostics, with intense background luminosity, scattering and beam obstruction from hot metal particles that can be as large as several hundred microns in diameter. CARS spectra that were previously obtained using nanosecond pulsed lasers in an aluminumparticle- seeded flame are examined and are determined to be severely impacted by nonresonant background, presumably as a result of themore » plasma formed by particulateenhanced laser-induced breakdown. Introduction of fs/ps laser pulses enables CARS detection at reduced pulse energies, decreasing the likelihood of breakdown, while simultaneously providing time-gated elimination of any nonresonant background interference. Temperature probability densities and temperature/oxygen correlations were constructed from ensembles of several thousand single-laser-shot measurements from the fs/ps rotational CARS measurement volume positioned within 3 mm or less of the burning propellant surface. Preliminary results in canonical flames are presented using a hybrid fs/ps vibrational CARS system to demonstrate our progress towards acquiring vibrational CARS measurements for more accurate temperatures in the very high temperature propellant burns.« less

Safety and Efficacy of Intratumoral Injections of Chimeric Antigen Receptor (CAR) T Cells in Metastatic Breast Cancer.

PubMed

Tchou, Julia; Zhao, Yangbing; Levine, Bruce L; Zhang, Paul J; Davis, Megan M; Melenhorst, Jan Joseph; Kulikovskaya, Irina; Brennan, Andrea L; Liu, Xiaojun; Lacey, Simon F; Posey, Avery D; Williams, Austin D; So, Alycia; Conejo-Garcia, Jose R; Plesa, Gabriela; Young, Regina M; McGettigan, Shannon; Campbell, Jean; Pierce, Robert H; Matro, Jennifer M; DeMichele, Angela M; Clark, Amy S; Cooper, Laurence J; Schuchter, Lynn M; Vonderheide, Robert H; June, Carl H

2017-12-01

Chimeric antigen receptors (CAR) are synthetic molecules that provide new specificities to T cells. Although successful in treatment of hematologic malignancies, CAR T cells are ineffective for solid tumors to date. We found that the cell-surface molecule c-Met was expressed in ∼50% of breast tumors, prompting the construction of a CAR T cell specific for c-Met, which halted tumor growth in immune-incompetent mice with tumor xenografts. We then evaluated the safety and feasibility of treating metastatic breast cancer with intratumoral administration of mRNA-transfected c-Met-CAR T cells in a phase 0 clinical trial (NCT01837602). Introducing the CAR construct via mRNA ensured safety by limiting the nontumor cell effects (on-target/off-tumor) of targeting c-Met. Patients with metastatic breast cancer with accessible cutaneous or lymph node metastases received a single intratumoral injection of 3 × 10 7 or 3 × 10 8 cells. CAR T mRNA was detectable in peripheral blood and in the injected tumor tissues after intratumoral injection in 2 and 4 patients, respectively. mRNA c-Met-CAR T cell injections were well tolerated, as none of the patients had study drug-related adverse effects greater than grade 1. Tumors treated with intratumoral injected mRNA c-Met-CAR T cells were excised and analyzed by immunohistochemistry, revealing extensive tumor necrosis at the injection site, cellular debris, loss of c-Met immunoreactivity, all surrounded by macrophages at the leading edges and within necrotic zones. We conclude that intratumoral injections of mRNA c-Met-CAR T cells are well tolerated and evoke an inflammatory response within tumors. Cancer Immunol Res; 5(12); 1152-61. ©2017 AACR . ©2017 American Association for Cancer Research.

Characterization and Functional Analysis of scFv-based Chimeric Antigen Receptors to Redirect T Cells to IL13Rα2-positive Glioma

PubMed Central

Krenciute, Giedre; Krebs, Simone; Torres, David; Wu, Meng-Fen; Liu, Hao; Dotti, Gianpietro; Li, Xiao-Nan; Lesniak, Maciej S; Balyasnikova, Irina V; Gottschalk, Stephen

2016-01-01

Immunotherapy with T cells expressing chimeric antigen receptors (CARs) is an attractive approach to improve outcomes for patients with glioblastoma (GBM). IL13Rα2 is expressed at a high frequency in GBM but not in normal brain, making it a promising CAR T-cell therapy target. IL13Rα2-specific CARs generated up to date contain mutated forms of IL13 as an antigen-binding domain. While these CARs target IL13Rα2, they also recognize IL13Rα1, which is broadly expressed. To overcome this limitation, we constructed a panel of IL13Rα2-specific CARs that contain the IL13Rα2-specific single-chain variable fragment (scFv) 47 as an antigen binding domain, short or long spacer regions, a transmembrane domain, and endodomains derived from costimulatory molecules and CD3.ζ (IL13Rα2-CARs). IL13Rα2-CAR T cells recognized IL13Rα2-positive target cells in coculture and cytotoxicity assays with no cross-reactivity to IL13Rα1. However, only IL13Rα2-CAR T cells with a short spacer region produced IL2 in an antigen-dependent fashion. In vivo, T cells expressing IL13Rα2-CARs with short spacer regions and CD28.ζ, 41BB.ζ, and CD28.OX40.ζ endodomains had potent anti-glioma activity conferring a significant survival advantage in comparison to mice that received control T cells. Thus, IL13Rα2-CAR T cells hold the promise to improve current IL13Rα2-targeted immunotherapy approaches for GBM and other IL13Rα2-positive malignancies. PMID:26514825

49 CFR 179.400-21 - Test of pressure relief valves.

Code of Federal Regulations, 2011 CFR

2011-10-01

... HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specification for Cryogenic Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and...

The effects of transport by car on coagulation tests.

PubMed

Ergin, Merve; Erdogan, Serpil; Akturk, Onur; Erel, Ozcan

2017-10-26

This research investigated the effects of the transport of blood samples between centers/laboratories by car on coagulation tests. Five tubes of blood samples were taken from 20 healthy volunteers. The samples consisted of a baseline (control) group, centrifuged and noncentrifuged transported samples; centrifuged and noncentrifuged untransported samples. The groups of centrifuged and noncentrifuged samples were transported by car for 2 h. The centrifuged and noncentrifuged untransported samples were incubated in the laboratory until the transported samples arrived. Prothrombin time (PT) and activated partial thromboplastin time (APTT) tests were conducted for all samples. Significant differences between the baseline group and the centrifuged and noncentrifuged transported samples and the noncentrifuged untransported samples were found for APTT levels (p<0.05, for all). In addition, significant mean percentage differences in PT values were found between the baseline group and the noncentrifuged transported samples (p<0.001) and the noncentrifuged untransported samples (p=0.005). The mean level of PT in the noncentrifuged transported samples was outside the upper limit of the clinical decision level. Noncentrifuged transported samples showed clinically significant differences in PT test results that may have stemmed from mechanical agitation during transportation. Therefore, we recommend not transporting noncentrifuged specimens for PT testing by car.

Fuel Savings and Aerodynamic Drag Reduction from Rail Car Covers

NASA Technical Reports Server (NTRS)

Storms, Bruce; Salari, Kambiz; Babb, Alex

2008-01-01

The potential for energy savings by reducing the aerodynamic drag of rail cars is significant. A previous study of aerodynamic drag of coal cars suggests that a 25% reduction in drag of empty cars would correspond to a 5% fuel savings for a round trip [1]. Rail statistics for the United States [2] report that approximately 5.7 billion liters of diesel fuel were consumed for coal transportation in 2002, so a 5% fuel savings would total 284 million liters. This corresponds to 2% of Class I railroad fuel consumption nationwide. As part of a DOE-sponsored study, the aerodynamic drag of scale rail cars was measured in a wind tunnel. The goal of the study was to measure the drag reduction of various rail-car cover designs. The cover designs tested yielded an average drag reduction of 43% relative to empty cars corresponding to an estimated round-trip fuel savings of 9%.

Automatic license plate reader: a solution to avoiding vehicle pursuit

NASA Astrophysics Data System (ADS)

Jordan, Stanley K.

1997-01-01

The Massachusetts Governor's Auto Theft Strike Force has tested an automatic license plate reader (LPR) to recover stolen cars and catch car thieves, without vehicle pursuit. Experiments were conducted at the Sumner Tunnel in Boston, and proved the feasibility of a LPR for identifying stolen cars instantly. The same technology can be applied to other law-enforcement objectives.

Aerodynamics of Race Cars

NASA Astrophysics Data System (ADS)

Katz, Joseph

2006-01-01

Race car performance depends on elements such as the engine, tires, suspension, road, aerodynamics, and of course the driver. In recent years, however, vehicle aerodynamics gained increased attention, mainly due to the utilization of the negative lift (downforce) principle, yielding several important performance improvements. This review briefly explains the significance of the aerodynamic downforce and how it improves race car performance. After this short introduction various methods to generate downforce such as inverted wings, diffusers, and vortex generators are discussed. Due to the complex geometry of these vehicles, the aerodynamic interaction between the various body components is significant, resulting in vortex flows and lifting surface shapes unlike traditional airplane wings. Typical design tools such as wind tunnel testing, computational fluid dynamics, and track testing, and their relevance to race car development, are discussed as well. In spite of the tremendous progress of these design tools (due to better instrumentation, communication, and computational power), the fluid dynamic phenomenon is still highly nonlinear, and predicting the effect of a particular modification is not always trouble free. Several examples covering a wide range of vehicle shapes (e.g., from stock cars to open-wheel race cars) are presented to demonstrate this nonlinear nature of the flow field.

CARS technique for geological exploration of hydrocarbons deposits

NASA Astrophysics Data System (ADS)

Zhevlakov, A. P.; Bespalov, Victor; Elizarov, V. V.; Grishkanich, A. S.; Kascheev, S. V.; Makarov, E. A.; Bogoslovsky, S. A.; Il'inskiy, A. A.

2014-10-01

We developed a Raman lidar with ultraspectral resolution for automatic airborne monitoring of pipeline leaks and for oil and gas exploration. Experiments were carried out under the CARS circuit. Minimal concentrations of 200 ppb of heavy hydrocarbon gas have been remotely measured in laboratory tests. Test flights indicate that a sensitivity of 6 ppm for methane and 2 ppm for hydrogen sulfide has been reached for leakage detection. As estimations have shown the reliability of heavy hydrocarbon gas detection by the integration method of seismic prospecting and remote laser sensing in CARS circuit can exceed 80%.

Evaluation of Exhaust Emissions from Three Diesel-Hybrid Cars and Simulation of After-Treatment Systems for Ultralow Real-World NOx Emissions.

PubMed

Franco, Vicente; Zacharopoulou, Theodora; Hammer, Jan; Schmidt, Helge; Mock, Peter; Weiss, Martin; Samaras, Zissis

2016-12-06

Hybridization offers great potential for decreasing pollutant and carbon dioxide emissions of diesel cars. However, an assessment of the real-world emissions performance of modern diesel hybrids is missing. Here, we test three diesel-hybrid cars on the road and benchmark our findings with two cars against tests on the chassis dynamometer and model simulations. The pollutant emissions of the two cars tested on the chassis dynamometer were in compliance with the relevant Euro standards over the New European Driving Cycle and Worldwide harmonized Light vehicles Test Procedure. On the road, all three diesel-hybrids exceeded the regulatory NO x limits (average exceedance for all trips: +150% for the Volvo, +510% for the Peugeot, and +550% for the Mercedes-Benz) and also showed elevated on-road CO 2 emissions (average exceedance of certification values: +178, +77, and +52%, respectively). These findings point to a wide discrepancy between certified and on-road CO 2 and suggest that hybridization alone is insufficient to achieve low-NO x emissions of diesel powertrains. Instead, our simulation suggests that properly calibrated selective catalytic reduction filter and lean-NO x trap after-treatment technologies can reduce the on-road NO x emissions to 0.023 and 0.068 g/km on average, respectively, well below the Euro 6 limit (0.080 g/km).

At the Bench: Chimeric antigen receptor (CAR) T cell therapy for the treatment of B cell malignancies.

PubMed

Daniyan, Anthony F O; Brentjens, Renier J

2016-12-01

The chimeric antigen receptor (CAR) represents the epitome of cellular engineering and is one of the best examples of rational biologic design of a synthetic molecule. The CAR is a single polypeptide with modular domains, consisting of an antibody-derived targeting moiety, fused in line with T cell-derived signaling domains, allowing for T cell activation upon ligand binding. T cells expressing a CAR are able to eradicate selectively antigen-expressing tumor cells in a MHC-independent fashion. CD19, a tumor-associated antigen (TAA) present on normal B cells, as well as most B cell-derived malignancies, was an early target of this technology. Through years of experimental refinement and preclinical optimization, autologously derived CD19-targeting CAR T cells have been successfully, clinically deployed, resulting in dramatic and durable antitumor responses but not without therapy-associated toxicity. As CD19-targeted CAR T cells continue to show clinical success, work at the bench continues to be undertaken to increase further the efficacy of this therapy, while simultaneously minimizing the risk for treatment-related morbidities. In this review, we cover the history and evolution of CAR technology and its adaptation to targeting CD19. Furthermore, we discuss the future of CAR T cell therapy and the need to ask, as well as answer, critical questions as this treatment modality is being translated to the clinic. © Society for Leukocyte Biology.

49 CFR Appendix A to Part 179 - Procedures for Tank-Head Puncture-Resistance Test

Code of Federal Regulations, 2013 CFR

2013-10-01

...” car. c. At least two separate tests must be conducted with the coupler on the vertical centerline of the ram car. One test must be conducted with the coupler at a height of 53.3 cm (21 inches), plus-or... height at 79 cm (31 inches), plus-or-minus 2.5 cm (1 inch), above the top of the sill. If the combined...

Adaptive Airpower: Arming America for the Future Through 4D Printing

DTIC Science & Technology

2015-05-01

airpower for continued dominance into the future. 3 Introduction The year is 2040; people still do not own flying cars , oil is still...manufactured with a printer capable of precise resolution down to 100 nanometers. This car is approximately 300 nanometers long and 100 nanometers wide...program, partnered between MIT, Airbus, and others, “a single piece of programmable carbon fiber transforms its shape to create aerodynamic advantage and

In vivo single human sweat gland activity monitoring using coherent anti-Stokes Raman scattering and two-photon excited autofluorescence microscopy.

PubMed

Chen, X; Gasecka, P; Formanek, F; Galey, J-B; Rigneault, H

2016-04-01

Eccrine sweat secretion is of central importance for control of body temperature. Although the incidence of sweat gland dysfunction might appear of minor importance, it can be a real concern for people with either hypohidrosis or hyperhidrosis. However, sweat gland function remains relatively poorly explored. To investigate the function of single human sweat glands. We describe a new approach for noninvasive imaging of single sweat gland activity in human palms in vivo up to a depth of 100 μm, based on nonlinear two-photon excited autofluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS). These techniques appear to be useful compared with approaches already described for imaging single sweat gland activity, as they allow better three-dimensional spatial resolution of sweat pore inner morphology and real-time monitoring of individual sweat events. By filling the sweat pore with oil and tuning the CARS contrast at 2845 cm(-1) , we imaged the ejection of sweat droplets from a single sweat gland when oil is pushed out by sweat flow. On average, sweat events lasted for about 30 s every 3 min under the conditions studied. On the other hand, about 20% of sweat glands were found inactive. TPEF and CARS were also used to study, at the single pore level, the antiperspirant action of aluminium chlorohydrate (ACH) and to reveal, for the first time in vivo, the formation of a plug at the pore entrance, in agreement with reported ACH antiperspirant mechanisms. Although data were acquired on human palms, these techniques show great promise for a better understanding of sweat secretion physiology and should be helpful to improve the efficacy of antiperspirant formulations. © 2015 British Association of Dermatologists.

Excited Ejecta in Light of Sight from Eta Car

NASA Technical Reports Server (NTRS)

Vieira, G.; Gull, T. R.; Danks, A.

2003-01-01

In the NUV spectrum of Eta Car, we have resolved many narrow absorption lines of neutral and singly-ionized elements with the Space Telescope Imaging Spectrograph. We report for the first time the detection of interstellar vanadium in absorption, and many highly-excited absorption lines of Fe, Cr, Ti, Ni, Co, Mn, and Mg. These elements, normally tied up in dust grains in the ISM, are located within wall of the Homunculus within 20,000 A.U. of Eta Car. Stellar radiation and stellar wind are interacting with the wall. Dust is likely being modified and/or destroyed. Previous Homunculus studies have demonstrated that nitrogen is overabundant and that carbon and oxygen emission lines are weak, or non-existent. Are the large column densities of these heavy elements due to abundance effects, excitation mechanisms, or modified grains? We may gain insight as Eta Car goes through its spectroscopic minimum in the summer of 2003.

Dual-pump CARS of Air in a Heated Pressure Vessel up to 55 Bar and 1300 K

NASA Technical Reports Server (NTRS)

Cantu, Luca; Gallo, Emanuela; Cutler, Andrew D.; Danehy, Paul M.

2014-01-01

Dual-pump Coherent anti-Stokes Raman scattering (CARS) measurements have been performed in a heated pressure vessel at NASA Langley Research Center. Each measurement, consisting of 500 single shot spectra, was recorded at a fixed location in dry air at various pressures and temperatures, in a range of 0.03-55×10(exp 5) Pa and 300-1373 K, where the temperature was varied using an electric heater. The maximum output power of the electric heater limited the combinations of pressures and temperatures that could be obtained. Charts of CARS signal versus temperature (at constant pressure) and signal versus pressure (at constant temperature) are presented and fit with an empirical model to validate the range of capability of the dual-pump CARS technique; averaged spectra at different conditions of pressure and temperature are also shown.

Separate and unsanitary: African American women railroad car cleaners and the Women's Service Section, 1918-1920.

PubMed

Muhammad, Robin Dearmon

2011-01-01

The Women's Service Section (WSS) investigated federally controlled railroad stations and yards at the end of World War I. Few women worked in car cleaning before the war, and railroad management preferred to block women workers, especially African Americans, from gaining any kind of foothold in railroad work. African American women were the single largest group of railroad car cleaners during this period but they were routinely denied adequate facilities, including toilets, locker rooms, and dining facilities throughout the railroad system. By raising the issues of facilities, workers' rights, and public health, these women shaped federal policy and widened the agenda of the WSS to include a direct attack on segregated workplaces. This article argues that African American women car cleaners launched an industrial campaign that wove together concerns about racism, sexism, and health issues, and successfully removed barriers to women working in a predominately male industry.

A Mechanical Harmony to NASA's Webb Telescope Sunshield

NASA Image and Video Library

2017-12-08

NASA's James Webb Space Telescope has a giant custom-built, kite-shaped sunshield driven by mechanics that will fold and unfold with a harmonious synchronicity 1 million miles from Earth. Like a car, many mechanical pieces in the Webb telescope's sunshield will work together to open it from its stored folded position in the rocket that will carry it into space. According to car manufacturers, a single car can have about 30,000 parts, counting every part down to the smallest screws. Like getting all of the parts in a car to operate together, the mechanical parts of the sunshield have to work in the same way. The sunshield support structure contains well over 7,000 flight parts, including springs, bearings, pulleys, magnets, etc. In addition, the sunshield has hundreds of custom fabricated pieces. Most mechanical pieces were developed exclusively for the sunshield, with a few from existing designs. Read more: go.nasa.gov/2cXcQMT

Rational development and characterization of humanized anti-EGFR variant III chimeric antigen receptor T cells for glioblastoma.

PubMed

Johnson, Laura A; Scholler, John; Ohkuri, Takayuki; Kosaka, Akemi; Patel, Prachi R; McGettigan, Shannon E; Nace, Arben K; Dentchev, Tzvete; Thekkat, Pramod; Loew, Andreas; Boesteanu, Alina C; Cogdill, Alexandria P; Chen, Taylor; Fraietta, Joseph A; Kloss, Christopher C; Posey, Avery D; Engels, Boris; Singh, Reshma; Ezell, Tucker; Idamakanti, Neeraja; Ramones, Melissa H; Li, Na; Zhou, Li; Plesa, Gabriela; Seykora, John T; Okada, Hideho; June, Carl H; Brogdon, Jennifer L; Maus, Marcela V

2015-02-18

Chimeric antigen receptors (CARs) are synthetic molecules designed to redirect T cells to specific antigens. CAR-modified T cells can mediate long-term durable remissions in B cell malignancies, but expanding this platform to solid tumors requires the discovery of surface targets with limited expression in normal tissues. The variant III mutation of the epidermal growth factor receptor (EGFRvIII) results from an in-frame deletion of a portion of the extracellular domain, creating a neoepitope. We chose a vector backbone encoding a second-generation CAR based on efficacy of a murine scFv-based CAR in a xenograft model of glioblastoma. Next, we generated a panel of humanized scFvs and tested their specificity and function as soluble proteins and in the form of CAR-transduced T cells; a low-affinity scFv was selected on the basis of its specificity for EGFRvIII over wild-type EGFR. The lead candidate scFv was tested in vitro for its ability to direct CAR-transduced T cells to specifically lyse, proliferate, and secrete cytokines in response to antigen-bearing targets. We further evaluated the specificity of the lead CAR candidate in vitro against EGFR-expressing keratinocytes and in vivo in a model of mice grafted with normal human skin. EGFRvIII-directed CAR T cells were also able to control tumor growth in xenogeneic subcutaneous and orthotopic models of human EGFRvIII(+) glioblastoma. On the basis of these results, we have designed a phase 1 clinical study of CAR T cells transduced with humanized scFv directed to EGFRvIII in patients with either residual or recurrent glioblastoma (NCT02209376). Copyright © 2015, American Association for the Advancement of Science.

75 FR 52326 - Agency Information Collection Activities; Proposed Collection; Comment Request; EPA's Light-Duty...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-25

... has an ongoing program to evaluate the emission performance of in-use light-duty (passenger car and... number'' of any class or category of vehicles or engines, although properly maintained and used, do not... selects approximately 50 classes of passenger cars and light trucks for in-use testing, at EPA's testing...

49 CFR 179.200-23 - Tests of pressure relief valves.

Code of Federal Regulations, 2010 CFR

2010-10-01

... MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specifications for Non-Pressure Tank Car Tanks (Classes DOT-111AW and 115AW) § 179.200...

49 CFR 180.511 - Acceptable results of inspections and tests.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) Safety system inspection. A tank car successfully passes the safety system inspection when each thermal..., distortion, excessive permanent expansion, or other evidence of weakness that might render the tank car...

The use of telemetry in testing in high performance racing engines

NASA Astrophysics Data System (ADS)

Hauser, E.

Telemetry measurement data in mobile application and under difficult environmental conditions were recorded. All relevant racing car and engine parameters were measured: pressure, stress, temperature, acceleration, ignition, number of revolutions, control of electronic injection, and flow measurements on the car body. The difficult measuring conditions due to high voltage ignition, mechanical loads and vibrations impose special requirements on a telemetry system built in racing cars. It has to be compact, flexible, light, and mechanically robust and has to fulfil special sheilding conditions. The measured data are transfered to a stationary measurement car via a radio line, involving RF communication problems. The measured data are directly displayed and evaluated in the measurement car.

CARS hyperspectral imaging of cartilage aiming for state discrimination of cell

NASA Astrophysics Data System (ADS)

Shiozawa, Manabu; Shirai, Masataka; Izumisawa, Junko; Tanabe, Maiko; Watanabe, Koichi

2016-03-01

Non-invasive cell analyses are increasingly important for medical field. A CARS microscope is one of the non-invasive imaging equipments and enables to obtain images indicating molecular distribution. Some studies on discrimination of cell state by using CARS images of lipid are reported. However, due to low signal intensity, it is still challenging to obtain images of the fingerprint region (800~1800 cm-1), in which many spectrum peaks correspond to compositions of a cell. Here, to identify cell differentiation by using multiplex CARS, we investigated hyperspectral imaging of fingerprint region of living cells. To perform multiplex CARS, we used a prototype of a compact light source, which consists of a microchip laser, a single-mode fiber, and a photonic crystal fiber to generate supercontinuum light. Assuming application to regenerative medicine, we chose a cartilage cell, whose differentiation is difficult to be identified by change of the cell morphology. Because one of the major components of cartilage is collagen, we focused on distribution of proline, which accounts for approximately 20% of collagen in general. The spectrum quality was improved by optical adjustments about power branching ratio and divergence of broadband Stokes light. Hyperspectral images were successfully obtained by the improvement. Periphery of a cartilage cell was highlighted in CARS image of proline, and this result suggests correspondence with collagen generated as extracellular matrix. A possibility of cell analyses by using CARS hyperspectral imaging was indicated.

A chimeric antigen receptor for TRAIL-receptor 1 induces apoptosis in various types of tumor cells.

PubMed

Kobayashi, Eiji; Kishi, Hiroyuki; Ozawa, Tatsuhiko; Hamana, Hiroshi; Nakagawa, Hidetoshi; Jin, Aishun; Lin, Zhezhu; Muraguchi, Atsushi

2014-10-31

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and its associated receptors (TRAIL-R/TR) are attractive targets for cancer therapy because TRAIL induces apoptosis in tumor cells through TR while having little cytotoxicity on normal cells. Therefore, many agonistic monoclonal antibodies (mAbs) specific for TR have been produced, and these induce apoptosis in multiple tumor cell types. However, some TR-expressing tumor cells are resistant to TR-specific mAb-induced apoptosis. In this study, we constructed a chimeric antigen receptor (CAR) of a TRAIL-receptor 1 (TR1)-specific single chain variable fragment (scFv) antibody (TR1-scFv-CAR) and expressed it on a Jurkat T cell line, the KHYG-1 NK cell line, and human peripheral blood lymphocytes (PBLs). We found that the TR1-scFv-CAR-expressing Jurkat cells killed target cells via TR1-mediated apoptosis, whereas TR1-scFv-CAR-expressing KHYG-1 cells and PBLs killed target cells not only via TR1-mediated apoptosis but also via CAR signal-induced cytolysis, resulting in cytotoxicity on a broader range if target cells than with TR1-scFv-CAR-expressing Jurkat cells. The results suggest that TR1-scFv-CAR could be a new candidate for cancer gene therapy. Copyright © 2014 Elsevier Inc. All rights reserved.

General object recognition is specific: Evidence from novel and familiar objects.

PubMed

Richler, Jennifer J; Wilmer, Jeremy B; Gauthier, Isabel

2017-09-01

In tests of object recognition, individual differences typically correlate modestly but nontrivially across familiar categories (e.g. cars, faces, shoes, birds, mushrooms). In theory, these correlations could reflect either global, non-specific mechanisms, such as general intelligence (IQ), or more specific mechanisms. Here, we introduce two separate methods for effectively capturing category-general performance variation, one that uses novel objects and one that uses familiar objects. In each case, we show that category-general performance variance is unrelated to IQ, thereby implicating more specific mechanisms. The first approach examines three newly developed novel object memory tests (NOMTs). We predicted that NOMTs would exhibit more shared, category-general variance than familiar object memory tests (FOMTs) because novel objects, unlike familiar objects, lack category-specific environmental influences (e.g. exposure to car magazines or botany classes). This prediction held, and remarkably, virtually none of the substantial shared variance among NOMTs was explained by IQ. Also, while NOMTs correlated nontrivially with two FOMTs (faces, cars), these correlations were smaller than among NOMTs and no larger than between the face and car tests themselves, suggesting that the category-general variance captured by NOMTs is specific not only relative to IQ, but also, to some degree, relative to both face and car recognition. The second approach averaged performance across multiple FOMTs, which we predicted would increase category-general variance by averaging out category-specific factors. This prediction held, and as with NOMTs, virtually none of the shared variance among FOMTs was explained by IQ. Overall, these results support the existence of object recognition mechanisms that, though category-general, are specific relative to IQ and substantially separable from face and car recognition. They also add sensitive, well-normed NOMTs to the tools available to study object recognition. Copyright © 2017 Elsevier B.V. All rights reserved.

Third-generation CD28/4-1BB chimeric antigen receptor T cells for chemotherapy relapsed or refractory acute lymphoblastic leukaemia: a non-randomised, open-label phase I trial protocol.

PubMed

Tang, Xiao-Yi; Sun, Yao; Zhang, Ang; Hu, Guo-Liang; Cao, Wei; Wang, Dan-Hong; Zhang, Bin; Chen, Hu

2016-12-30

There is no curative treatment available for patients with chemotherapy relapsed or refractory CD19+ B cells-derived acute lymphoblastic leukaemia (r/r B-ALL). Although CD19-targeting second-generation (2nd-G) chimeric antigen receptor (CAR)-modified T cells carrying CD28 or 4-1BB domains have demonstrated potency in patients with advanced B-ALL, these 2 signalling domains endow CAR-T cells with different and complementary functional properties. Preclinical results have shown that third-generation (3rd-G) CAR-T cells combining 4-1BB and CD28 signalling domains have superior activation and proliferation capacity compared with 2nd-G CAR-T cells carrying CD28 domain. The aim of the current study is therefore to investigate the safety and efficacy of 3rd-G CAR-T cells in adults with r/r B-ALL. This study is a phase I clinical trial for patients with r/r B-ALL to test the safety and preliminary efficacy of 3rd-G CAR-T cells. Before receiving lymphodepleting conditioning regimen, the peripheral blood mononuclear cells from eligible patients will be leukapheresed, and the T cells will be purified, activated, transduced and expanded ex vivo. On day 6 in the protocol, a single dose of 1 million CAR-T cells per kg will be administrated intravenously. The phenotypes of infused CAR-T cells, copy number of CAR transgene and plasma cytokines will be assayed for 2 years after CAR-T infusion using flow cytometry, real-time quantitative PCR and cytometric bead array, respectively. Moreover, several predictive plasma cytokines including interferon-γ, interleukin (IL)-6, IL-8, Soluble Interleukin (sIL)-2R-α, solubleglycoprotein (sgp)130, sIL-6R, Monocyte chemoattractant protein (MCP1), Macrophage inflammatory protein (MIP1)-α, MIP1-β and Granulocyte-macrophage colony-stimulating factor (GM-CSF), which are highly associated with severe cytokine release syndrome (CRS), will be used to forecast CRS to allow doing earlier intervention, and CRS will be managed based on a revised CRS grading system. In addition, patients with grade 3 or 4 neurotoxicities or persistent B-cell aplasia will be treated with dexamethasone (10 mg intravenously every 6 hours) or IgG, respectively. Descriptive and analytical analyses will be performed. Ethical approval for the study was granted on 10 July 2014 (YLJS-2014-7-10). Written informed consent will be taken from all participants. The results of the study will be reported, through peer-reviewed journals, conference presentations and an internal organisational report. NCT02186860. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

Learning for VMM + WTA Embedded Classifiers

DTIC Science & Technology

2016-03-31

enabling correct classification of each novel acoustic signal (generator, idle car , and idle truck). The classification structure requires, after...measured on our SoC FPAA IC. The test input is composed of signals from urban environment for 3 objects (generator, idle car , and idle truck...classifier results from a rural truck data set, an urban generator set, and urban idle car dataset. Solid lines represent our extracted background

40 CFR 63.2346 - What emission limitations, operating limits, and work practice standards must I meet?

Code of Federal Regulations, 2010 CFR

2010-07-01

... Department of Transportation (U.S. DOT) pressure test requirements of 49 CFR part 180 for cargo tanks and 49 CFR 173.31 for tank cars. (iii) Organic liquids must only be unloaded from cargo tanks or tank cars... pressure relief device on the storage tank, or on the cargo tank or tank car, shall open during loading or...

40 CFR 63.2346 - What emission limitations, operating limits, and work practice standards must I meet?

Code of Federal Regulations, 2011 CFR

2011-07-01

... Department of Transportation (U.S. DOT) pressure test requirements of 49 CFR part 180 for cargo tanks and 49 CFR 173.31 for tank cars. (iii) Organic liquids must only be unloaded from cargo tanks or tank cars... pressure relief device on the storage tank, or on the cargo tank or tank car, shall open during loading or...

Numerical calculation of boundary layers and wake characteristics of high-speed trains with different lengths

PubMed Central

Zhou, Dan; Niu, Jiqiang

2017-01-01

Trains with different numbers of cars running in the open air were simulated using the delayed detached-eddy simulation (DDES). The numbers of cars included in the simulation are 3, 4, 5 and 8. The aim of this study was to investigate how train length influences the boundary layer, the wake flow, the surface pressure, the aerodynamic drag and the friction drag. To certify the accuracy of the mesh and methods, the drag coefficients from numerical simulation of trains with 3 cars were compared with those from the wind tunnel test, and agreement was obtained. The results show that the boundary layer is thicker and the wake vortices are less symmetric as the train length increases. As a result, train length greatly affects pressure. The upper surface pressure of the tail car reduced by 2.9%, the side surface pressure of the tail car reduced by 8.3% and the underneath surface pressure of the tail car reduced by 19.7% in trains that included 3 cars to those including 8 cars. In addition, train length also has a significant effect on the friction drag coefficient and the drag coefficient. The friction drag coefficient of each car in a configuration decreases along the length of the train. In a comparison between trains consisting of 3 cars to those consisting of 8 cars, the friction drag coefficient of the tail car reduced by 8.6% and the drag coefficient of the tail car reduced by 3.7%. PMID:29261758

Serum Oxytocin Levels and an Oxytocin Receptor Gene Polymorphism (rs2254298) Indicate Social Deficits in Children and Adolescents with Autism Spectrum Disorders

PubMed Central

Yang, Shuhan; Dong, Xiaopeng; Guo, Xuan; Han, Yu; Song, Hanbing; Gao, Lei; Dai, Wei; Su, Yuanyuan; Zhang, Xin

2017-01-01

The neuropeptide oxytocin (OT) and its receptor (OXTR) have been predicted to be involved in the regulation of social functioning in autism spectrum disorders (ASD). Objective of the study was to investigate serum OT levels and the OXTR rs2254298 polymorphism in Chinese Han children and adolescents with ASD as well as to identify their social deficits relevant to the oxytocinergic system. We tested serum OT levels using ELISA in 55 ASD subjects and 110 typically developing (TD) controls as well as genotyped the OXTR rs2254298 polymorphism using PCR-RFLP in 100 ASD subjects and 232 TD controls. Autistic symptoms were assessed by the Autism Behavior Checklist (ABC) and the Childhood Autism Rating Scale (CARS). There were no significant associations between OXTR rs2254298 polymorphism and ASD, serum OT levels and age, as well as serum OT levels and intelligent quotient (IQ) in both ASD and TD groups. However, ASD subjects exhibited elevated serum OT levels compared to TD controls and positive correlations between serum OT levels and “adaptation to change score” in the CARS and CARS total scores. Moreover, in the ASD group, significant relationships were revealed between the single-nucleotide polymorphism (SNP) rs2254298 and serum OT levels, the category “stereotypes and object use” in the ABC and the category “adaptation to change” in the CARS. These findings indicated that individuals with ASD may exhibit a dysregulation in OT on the basis of changes in OXTR gene expression as well as environmentally induced alterations of the oxytocinergic system to determine their social deficits. PMID:28484366

The contribution of cognition and spasticity to driving performance in multiple sclerosis.

PubMed

Marcotte, Thomas D; Rosenthal, Theodore J; Roberts, Erica; Lampinen, Sara; Scott, J Cobb; Allen, R Wade; Corey-Bloom, Jody

2008-09-01

To examine the independent and combined impact of cognitive dysfunction and spasticity on driving tasks involving high cognitive workload and lower-limb mobility in persons with multiple sclerosis (MS). Single-visit cohort study. Clinical research center. Participants included 17 drivers with MS and 14 referent controls. The group with MS exhibited a broad range of cognitive functioning and disability. Of the 17 patients with MS, 8 had significant spasticity in the knee used to manipulate the accelerator and brake pedals (based on the Modified Ashworth Scale). Not applicable. A brief neuropsychologic test battery and 2 driving simulations. Simulation 1 required participants to maintain a constant speed and lane position while attending to a secondary task. Simulation 2 required participants to adjust their speed to accelerations and decelerations of a lead car in front of them. Patients with MS showed greater variability in lane position (effect size, g=1.30), greater difficulty in maintaining a constant speed (g=1.25), and less ability to respond to lead car speed changes (g=1.85) compared with controls. Within the MS group, in a multivariate model that included neuropsychologic and spasticity measures, cognitive functioning was the strongest predictor of difficulty in maintaining lane position during the divided attention task and poor response time to lead car speed changes, whereas spasticity was associated with reductions in accuracy of tracking the lead car movements and speed maintenance. In this preliminary study, cognitive and physical impairments associated with MS were related to deficits in specific components of simulated driving. Assessment of these factors may help guide the clinician regarding the types of driving behaviors that would put patients with MS at an increased risk for an automobile crash.

The Contribution of Cognition and Spasticity to Driving Performance in Multiple Sclerosis

PubMed Central

Marcotte, Thomas D.; Rosenthal, Theodore J.; Roberts, Erica; Lampinen, Sara; Scott, J. Cobb; Allen, R. Wade; Corey-Bloom, Jody

2014-01-01

Objective To examine the independent and combined impact of cognitive dysfunction and spasticity on driving tasks involving high cognitive workload and lower-limb mobility in individuals with multiple sclerosis (MS). Design Single-visit cohort study. Setting Clinical research center. Participants Seventeen drivers with MS and 14 normal controls. The MS group exhibited a broad range of cognitive functioning and disability. Eight MS patients had significant spasticity in the knee proximal to the pedals (based on the Modified Ashworth Scale). Interventions Not applicable. Main Outcome Measures A brief neuropsychologic test battery and 2 driving simulations. Simulation 1 required participants to maintain a constant speed and lane position while attending to a secondary task. Simulation 2 required participants to adjust their speed to accelerations and decelerations of a lead car in front of them. Results MS patients demonstrated greater variability in lane position (effect size g=1.30), greater difficulty in maintaining a constant speed (g=1.25), and less ability to respond to lead car speed changes (g=1.85) compared with controls. Within the MS group, in a multivariate model that included neuropsychologic and spasticity measures, cognitive functioning was the strongest predictor of difficulty in maintaining lane position during the divided attention task and poor response time to lead car speed changes, whereas spasticity was associated with reductions in accuracy of tracking the lead car movements and speed maintenance. Conclusions In this preliminary study, cognitive and physical impairments associated with MS were related to deficits in specific components of simulated driving, and assessment of these factors may help guide the clinician regarding the types of driving behaviors that would put MS patients at increased risk for a crash. PMID:18760160

A NARX damper model for virtual tuning of automotive suspension systems with high-frequency loading

NASA Astrophysics Data System (ADS)

Alghafir, M. N.; Dunne, J. F.

2012-02-01

A computationally efficient NARX-type neural network model is developed to characterise highly nonlinear frequency-dependent thermally sensitive hydraulic dampers for use in the virtual tuning of passive suspension systems with high-frequency loading. Three input variables are chosen to account for high-frequency kinematics and temperature variations arising from continuous vehicle operation over non-smooth surfaces such as stone-covered streets, rough or off-road conditions. Two additional input variables are chosen to represent tuneable valve parameters. To assist in the development of the NARX model, a highly accurate but computationally excessive physical damper model [originally proposed by S. Duym and K. Reybrouck, Physical characterization of non-linear shock absorber dynamics, Eur. J. Mech. Eng. M 43(4) (1998), pp. 181-188] is extended to allow for high-frequency input kinematics. Experimental verification of this extended version uses measured damper data obtained from an industrial damper test machine under near-isothermal conditions for fixed valve settings, with input kinematics corresponding to harmonic and random road profiles. The extended model is then used only for simulating data for training and testing the NARX model with specified temperature profiles and different valve parameters, both in isolation and within quarter-car vehicle simulations. A heat generation and dissipation model is also developed and experimentally verified for use within the simulations. Virtual tuning using the quarter-car simulation model then exploits the NARX damper to achieve a compromise between ride and handling under transient thermal conditions with harmonic and random road profiles. For quarter-car simulations, the paper shows that a single tuneable NARX damper makes virtual tuning computationally very attractive.

Adoptive Transfer of IL13Rα2-Specific Chimeric Antigen Receptor T Cells Creates a Pro-inflammatory Environment in Glioblastoma.

PubMed

Pituch, Katarzyna C; Miska, Jason; Krenciute, Giedre; Panek, Wojciech K; Li, Gina; Rodriguez-Cruz, Tania; Wu, Meijing; Han, Yu; Lesniak, Maciej S; Gottschalk, Stephen; Balyasnikova, Irina V

2018-04-04

In order to fully harness the potential of immunotherapy with chimeric antigen receptor (CAR)-modified T cells, pre-clinical studies must be conducted in immunocompetent animal models that closely mimic the immunosuppressive malignant glioma (MG) microenvironment. Thus, the goal of this project was to study the in vivo fate of T cells expressing CARs specific for the MG antigen IL13Rα2 (IL13Rα2-CARs) in immunocompetent MG models. Murine T cells expressing IL13Rα2-CARs with a CD28.ζ (IL13Rα2-CAR.CD28.ζ) or truncated signaling domain (IL13Rα2-CAR.Δ) were generated by retroviral transduction, and their effector function was evaluated both in vitro and in vivo. IL13Rα2-CAR.CD28.ζ T cells' specificity toward IL13Rα2 was confirmed through cytokine production and cytolytic activity. In vivo, a single intratumoral injection of IL13Rα2-CAR.CD28.ζ T cells significantly extended the survival of IL13Rα2-expressing GL261 and SMA560 glioma-bearing mice; long-term survivors were resistant to re-challenge with IL13Rα2-negative and IL13Rα2-positive tumors. IL13Rα2-CAR.CD28.ζ T cells proliferated, produced cytokines (IFNγ, TNF-α), and promoted a phenotypically pro-inflammatory glioma microenvironment by inducing a significant increase in the number of CD4 + and CD8 + T cells and CD8α + dendritic cells and a decrease in Ly6G + myeloid-derived suppressor cells (MDSCs). Our data underline the significance of CAR T cell studies in immunocompetent hosts and further validate IL13Rα2-CAR T cells as an efficacious therapeutic strategy for MG. Copyright © 2018 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.

Association between unsafe driving performance and cognitive-perceptual dysfunction in older drivers.

PubMed

Park, Si-Woon; Choi, Eun Seok; Lim, Mun Hee; Kim, Eun Joo; Hwang, Sung Il; Choi, Kyung-In; Yoo, Hyun-Chul; Lee, Kuem Ju; Jung, Hi-Eun

2011-03-01

To find an association between cognitive-perceptual problems of older drivers and unsafe driving performance during simulated automobile driving in a virtual environment. Cross-sectional study. A driver evaluation clinic in a rehabilitation hospital. Fifty-five drivers aged 65 years or older and 48 drivers in their late twenties to early forties. All participants underwent evaluation of cognitive-perceptual function and driving performance, and the results were compared between older and younger drivers. The association between cognitive-perceptual function and driving performance was analyzed. Cognitive-perceptual function was evaluated with the Cognitive Perceptual Assessment for Driving (CPAD), a computer-based assessment tool consisting of depth perception, sustained attention, divided attention, the Stroop test, the digit span test, field dependency, and trail-making test A and B. Driving performance was evaluated with use of a virtual reality-based driving simulator. During simulated driving, car crashes were recorded, and an occupational therapist observed unsafe performances in controlling speed, braking, steering, vehicle positioning, making lane changes, and making turns. Thirty-five older drivers did not pass the CPAD test, whereas all of the younger drivers passed the test. When using the driving simulator, a significantly greater number of older drivers experienced car crashes and demonstrated unsafe performance in controlling speed, steering, and making lane changes. CPAD results were associated with car crashes, steering, vehicle positioning, and making lane changes. Older drivers who did not pass the CPAD test are 4 times more likely to experience a car crash, 3.5 times more likely to make errors in steering, 2.8 times more likely to make errors in vehicle positioning, and 6.5 times more likely to make errors in lane changes than are drivers who passed the CPAD test. Unsafe driving performance and car crashes during simulated driving were more prevalent in older drivers than in younger drivers. Unsafe performance in steering, vehicle positioning, making lane changes, and car crashes were associated with cognitive-perceptual dysfunction. Copyright © 2011 American Academy of Physical Medicine and Rehabilitation. Published by Elsevier Inc. All rights reserved.

The effect on road safety of a modal shift from car to bicycle.

PubMed

Stipdonk, Henk; Reurings, Martine

2012-01-01

To describe and apply a method to assess the effect on road safety of a modal shift from cars to bicycles. Ten percent of all car trips shorter than 7.5 km were assumed to be replaced by bicycle trips. Single-vehicle and multivehicle crashes involving cars and/or bicycles were considered. The safety of car occupants and cyclists was taken into account as well as the safety of other road users involved in such crashes. The computations were carried out by age and gender. Assuming constant risk (casualties per distance traveled), the expected number of accidents is proportional to the mobility shift. Several types of risk were considered: the risk of being injured as a car driver or cyclist and the risk of being involved as a car driver or cyclist in a crash in which another road user is injured. The results indicated that the total gain of the modal shift was negative for fatalities, which means that there was a net increase in the number of fatalities. The modal shift was advantageous for young drivers and disadvantageous for elderly drivers. In addition, it was more positive for males than for females. The turning point was around the age of 35. For hospitalized casualties, due to the strong influence of the many hospitalized cyclists in nonmotorized vehicle crashes, there was a strong negative overall effect, and the modal shift resulted in a positive effect for 18- and 19-year-old males only. Overall, a small increase (up to 1%) in the number of cyclist fatalities and a greater increase of 3.5 percent in the number of inpatients was expected. The increase in casualties was mainly due to the proportion of single-vehicle bicycle crashes with serious injuries in relation to the total number of injured cyclists. The effect of the modal shift was shown to depend on age and gender, resulting in fewer casualties for younger drivers and for women. It is possible to provide a first approximation of the effect on road safety of a mobility shift from cars to bicycles. This approximation indicates that, in general, road safety does not benefit from this modal shift. The effect differs for gender and age groups. Elderly drivers are safer inside a car than on a bicycle. For the number of hospitalized casualties, the modal shift increases the number of casualties for practically all ages and both genders.

Chimeric antigen receptor containing ICOS signaling domain mediates specific and efficient antitumor effect of T cells against EGFRvIII expressing glioma

PubMed Central

2013-01-01

Background Adoptive transfer of chimeric antigen receptor (CAR)-modified T cells appears to be a promising immunotherapeutic strategy. CAR combines the specificity of antibody and cytotoxicity of cytotoxic T lymphocytes, enhancing T cells’ ability to specifically target antigens and to effectively kill cancer cells. Recent efforts have been made to integrate the costimulatory signals in the CAR to improve the antitumor efficacy. Epidermal growth factor receptor variant III (EGFRvIII) is an attractive therapeutic target as it frequently expresses in glioma and many other types of cancers. Our current study aimed to investigate the specific and efficient antitumor effect of T cells modified with CAR containing inducible costimulator (ICOS) signaling domain. Methods A second generation of EGFRvIII/CAR was generated and it contained the EGFRvIII single chain variable fragment, ICOS signaling domain and CD3ζ chain. Lentiviral EGFRvIII/CAR was prepared and human CD3+ T cells were infected by lentivirus encoding EGFRvIII/CAR. The expression of EGFRvIII/CAR on CD3+ T cells was confirmed by flow cytometry and Western blot. The functions of EGFRvIII/CAR+ T cells were evaluated using in vitro and in vivo methods including cytotoxicity assay, cytokine release assay and xenograft tumor mouse model. Results Chimeric EGFRvIIIscFv-ICOS-CD3ζ (EGFRvIII/CAR) was constructed and lentiviral EGFRvIII/CAR were made to titer of 106 TU/ml. The transduction efficiency of lentiviral EGFRvIII/CAR on T cells reached around 70% and expression of EGFRvIII/CAR protein was verified by immunoblotting as a band of about 57 kDa. Four hour 51Cr release assays demonstrated specific and efficient cytotoxicity of EGFRvIII/CAR+ T cells against EGFRvIII expressing U87 cells. A robust increase in the IFN-γ secretion was detected in the co-culture supernatant of the EGFRvIII/CAR+ T cells and the EGFRvIII expressing U87 cells. Intravenous and intratumor injection of EGFRvIII/CAR+ T cells inhibited the in vivo growth of the EGFRvIII expressing glioma cells. Conclusions Our study demonstrates that the EGFRvIII/CAR-modified T cells can destroy glioma cells efficiently in an EGFRvIII specific manner and release IFN-γ in an antigen dependent manner. The specific recognition and effective killing activity of the EGFRvIII-directed T cells with ICOS signaling domain lays a foundation for us to employ such approach in future cancer treatment. PMID:23656794

Chimeric antigen receptor containing ICOS signaling domain mediates specific and efficient antitumor effect of T cells against EGFRvIII expressing glioma.

PubMed

Shen, Chan-Juan; Yang, Yu-Xiu; Han, Ethan Q; Cao, Na; Wang, Yun-Fei; Wang, Yi; Zhao, Ying-Ying; Zhao, Li-Ming; Cui, Jian; Gupta, Puja; Wong, Albert J; Han, Shuang-Yin

2013-05-09

Adoptive transfer of chimeric antigen receptor (CAR)-modified T cells appears to be a promising immunotherapeutic strategy. CAR combines the specificity of antibody and cytotoxicity of cytotoxic T lymphocytes, enhancing T cells' ability to specifically target antigens and to effectively kill cancer cells. Recent efforts have been made to integrate the costimulatory signals in the CAR to improve the antitumor efficacy. Epidermal growth factor receptor variant III (EGFRvIII) is an attractive therapeutic target as it frequently expresses in glioma and many other types of cancers. Our current study aimed to investigate the specific and efficient antitumor effect of T cells modified with CAR containing inducible costimulator (ICOS) signaling domain. A second generation of EGFRvIII/CAR was generated and it contained the EGFRvIII single chain variable fragment, ICOS signaling domain and CD3ζ chain. Lentiviral EGFRvIII/CAR was prepared and human CD3+ T cells were infected by lentivirus encoding EGFRvIII/CAR. The expression of EGFRvIII/CAR on CD3+ T cells was confirmed by flow cytometry and Western blot. The functions of EGFRvIII/CAR+ T cells were evaluated using in vitro and in vivo methods including cytotoxicity assay, cytokine release assay and xenograft tumor mouse model. Chimeric EGFRvIIIscFv-ICOS-CD3ζ (EGFRvIII/CAR) was constructed and lentiviral EGFRvIII/CAR were made to titer of 106 TU/ml. The transduction efficiency of lentiviral EGFRvIII/CAR on T cells reached around 70% and expression of EGFRvIII/CAR protein was verified by immunoblotting as a band of about 57 kDa. Four hour 51Cr release assays demonstrated specific and efficient cytotoxicity of EGFRvIII/CAR+ T cells against EGFRvIII expressing U87 cells. A robust increase in the IFN-γ secretion was detected in the co-culture supernatant of the EGFRvIII/CAR+ T cells and the EGFRvIII expressing U87 cells. Intravenous and intratumor injection of EGFRvIII/CAR+ T cells inhibited the in vivo growth of the EGFRvIII expressing glioma cells. Our study demonstrates that the EGFRvIII/CAR-modified T cells can destroy glioma cells efficiently in an EGFRvIII specific manner and release IFN-γ in an antigen dependent manner. The specific recognition and effective killing activity of the EGFRvIII-directed T cells with ICOS signaling domain lays a foundation for us to employ such approach in future cancer treatment.

Effective Targeting of Multiple B-Cell Maturation Antigen-Expressing Hematological Malignances by Anti-B-Cell Maturation Antigen Chimeric Antigen Receptor T Cells.

PubMed

Friedman, Kevin M; Garrett, Tracy E; Evans, John W; Horton, Holly M; Latimer, Howard J; Seidel, Stacie L; Horvath, Christopher J; Morgan, Richard A

2018-05-01

B-cell maturation antigen (BCMA) expression has been proposed as a marker for the identification of malignant plasma cells in patients with multiple myeloma (MM). Nearly all MM tumor cells express BCMA, while normal tissue expression is restricted to plasma cells and a subset of mature B cells. Consistent BCMA expression was confirmed on MM biopsies (29/29 BCMA+), and it was further demonstrated that BCMA is expressed in a substantial number of lymphoma samples, as well as primary chronic lymphocytic leukemia B cells. To target BCMA using redirected autologous T cells, lentiviral vectors (LVV) encoding chimeric antigen receptors (CARs) were constructed with four unique anti-BCMA single-chain variable fragments, fused to the CD137 (4-1BB) co-stimulatory and CD3ζ signaling domains. One LVV, BB2121, was studied in detail, and BB2121 CAR-transduced T cells (bb2121) exhibited a high frequency of CAR + T cells and robust in vitro activity against MM cell lines, lymphoma cell lines, and primary chronic lymphocytic leukemia peripheral blood. Based on receptor quantification, bb2121 recognized tumor cells expressing as little as 222 BCMA molecules per cell. The in vivo pharmacology of anti-BCMA CAR T cells was studied in NSG mouse models of human MM, Burkitt lymphoma, and mantle cell lymphoma, where mice received a single intravenous administration of vehicle, control vector-transduced T cells, or anti-BCMA CAR-transduced T cells. In all models, the vehicle and control CAR T cells failed to inhibit tumor growth. In contrast, treatment with bb2121 resulted in rapid and sustained elimination of the tumors and 100% survival in all treatment models. Together, these data support the further development of anti-BCMA CAR T cells as a potential treatment for not only MM but also some lymphomas.

High-Affinity GD2-Specific CAR T Cells Induce Fatal Encephalitis in a Preclinical Neuroblastoma Model

PubMed Central

Richman, Sarah A.; Nunez-Cruz, Selene; Moghimi, Babak; Li, Lucy Z.; Gershenson, Zachary T.; Mourelatos, Zissimos; Barrett, David M.; Grupp, Stephan A.; Milone, Michael C.

2018-01-01

The GD2 ganglioside, which is abundant on the surface of neuroblastoma cells, is targeted by an FDA-approved therapeutic monoclonal antibody and is an attractive tumor-associated antigen for cellular immunotherapy. Chimeric antigen receptor (CAR)–modified T cells can have potent antitumor activity in B-cell malignancies, and trials to harness this cytolytic activity toward GD2 in neuroblastoma are under way. In an effort to enhance the antitumor activity of CAR T cells that target GD2, we generated variant CAR constructs predicted to improve the stability and the affinity of the GD2-binding, 14G2a-based, single-chain variable fragment (scFv) of the CAR and compared their properties in vivo. We included the E101K mutation of GD2 scFv (GD2-E101K) that has enhanced antitumor activity against a GD2+ human neuroblastoma xenograft in vivo. However, this enhanced antitumor efficacy in vivo was concomitantly associated with lethal central nervous system (CNS) toxicity comprised of extensive CAR T-cell infiltration and proliferation within the brain and neuronal destruction. The encephalitis was localized to the cerebellum and basal regions of the brain that display low amounts of GD2. Our results highlight the challenges associated with target antigens that exhibit shared expression on critical normal tissues. Despite the success of GD2-specific antibody therapies in the treatment of neuroblastoma, the fatal neurotoxicity of GD2-specific CAR T-cell therapy observed in our studies suggests that GD2 may be a difficult target antigen for CAR T-cell therapy without additional strategies that can control CAR T-cell function within the CNS. PMID:29180536

Hybrid femtosecond/picosecond rotational coherent anti-Stokes Raman scattering temperature and concentration measurements using two different picosecond-duration probes.

PubMed

Kearney, Sean P; Scoglietti, Daniel J; Kliewer, Christopher J

2013-05-20

A hybrid fs/ps pure-rotational CARS scheme is characterized in furnace-heated air at temperatures from 290 to 800 K. Impulsive femtosecond excitation is used to prepare a rotational Raman coherence that is probed with a ps-duration beam generated from an initially broadband fs pulse that is bandwidth limited using air-spaced Fabry-Perot etalons. CARS spectra are generated using 1.5- and 7.0-ps duration probe beams with corresponding coarse and narrow spectral widths. The spectra are fitted using a simple phenomenological model for both shot-averaged and single-shot measurements of temperature and oxygen mole fraction. Our single-shot temperature measurements exhibit high levels of precision and accuracy when the spectrally coarse 1.5-ps probe beam is used, demonstrating that high spectral resolution is not required for thermometry. An initial assessment of concentration measurements in air is also provided, with best results obtained using the higher resolution 7.0-ps probe. This systematic assessment of the hybrid CARS technique demonstrates its utility for practical application in low-temperature gas-phase systems.

40 CFR 86.129-80 - Road load power, test weight, and inertia weight class determination.

Code of Federal Regulations, 2012 CFR

2012-07-01

... power, test weight, and inertia weight class determination. (a) Flywheels, electrical or other means of... equipment exceeding 0.1 ft2 which are anticipated to be sold on more than 33 pct of the car line. Frontal... expected that more than 33 percent of a car line within an engine-system combination will be equipped with...

40 CFR 86.129-80 - Road load power, test weight, and inertia weight class determination.

Code of Federal Regulations, 2013 CFR

2013-07-01

... power, test weight, and inertia weight class determination. (a) Flywheels, electrical or other means of... equipment exceeding 0.1 ft2 which are anticipated to be sold on more than 33 pct of the car line. Frontal... expected that more than 33 percent of a car line within an engine-system combination will be equipped with...

40 CFR 86.129-80 - Road load power, test weight, and inertia weight class determination.

Code of Federal Regulations, 2010 CFR

2010-07-01

... power, test weight, and inertia weight class determination. (a) Flywheels, electrical or other means of... equipment exceeding 0.1 ft2 which are anticipated to be sold on more than 33 pct of the car line. Frontal... expected that more than 33 percent of a car line within an engine-system combination will be equipped with...

40 CFR 86.129-80 - Road load power, test weight, and inertia weight class determination.

Code of Federal Regulations, 2011 CFR

2011-07-01

... power, test weight, and inertia weight class determination. (a) Flywheels, electrical or other means of... equipment exceeding 0.1 ft2 which are anticipated to be sold on more than 33 pct of the car line. Frontal... expected that more than 33 percent of a car line within an engine-system combination will be equipped with...

Generation of Potent T-cell Immunotherapy for Cancer using DAP12-based, Multichain, Chimeric Immunoreceptors

PubMed Central

Wang, Enxiu; Wang, Liang-Chuan; Tsai, Ching-Yi; Bhoj, Vijay; Gershenson, Zack; Moon, Edmund; Newick, Kheng; Sun, Jing; Lo, Albert; Baradet, Timothy; Feldman, Michael D.; Barrett, David; Puré, Ellen; Albelda, Steven; Milone, Michael C.

2015-01-01

Chimeric antigen receptors (CAR) bearing an antigen-binding domain linked in cis to the cytoplasmic domains of CD3ζ and costimulatory receptors have provided a potent method for engineering T-cell cytotoxicity towards B-cell leukemia and lymphoma. However, resistance to immunotherapy due to loss of T-cell effector function remains a significant barrier, especially in solid malignancies. We describe an alternative chimeric immunoreceptor design in which we have fused a single-chain variable fragment for antigen recognition to the transmembrane and cytoplasmic domains of KIR2DS2, a stimulatory killer immunoglobulin-like receptor (KIR). We show that this simple, KIR-based CAR (KIR-CAR) triggers robust antigen-specific proliferation and effector function in vitro when introduced into human T cells with DAP12, an immunotyrosine-based activation motifs (ITAM)-containing adaptor. T cells modified to express a KIR-CAR and DAP12 exhibit superior antitumor activity compared to standard first and second generation CD3ζ-based CARs in a xenograft model of mesothelioma highly resistant to immunotherapy. The enhanced antitumor activity is associated with improved retention of chimeric immunoreceptor expression and improved effector function of isolated tumor-infiltrating lymphocytes. These results support the exploration of KIR-CARs for adoptive T-cell immunotherapy, particularly in immunotherapy-resistant solid tumors. PMID:25941351

49 CFR Appendix B to Part 232 - Part 232 Prior to May 31, 2001 as Clarified Effective April 10, 2002

Code of Federal Regulations, 2010 CFR

2010-10-01

... coupled to train, after which, an automatic brake application and release test of airbrakes on rear car... closing angle cocks for cutting off one or more cars from the rear end of train, automatic air brake must... automatic air brake must not be depended upon to hold a locomotive, cars or train, when standing on a grade...

49 CFR Appendix B to Part 232 - Part 232 Prior to May 31, 2001 as Clarified Effective April 10, 2002

Code of Federal Regulations, 2011 CFR

2011-10-01

... coupled to train, after which, an automatic brake application and release test of airbrakes on rear car... closing angle cocks for cutting off one or more cars from the rear end of train, automatic air brake must... automatic air brake must not be depended upon to hold a locomotive, cars or train, when standing on a grade...

T cells expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase 1 dose-escalation trial.

PubMed

Lee, Daniel W; Kochenderfer, James N; Stetler-Stevenson, Maryalice; Cui, Yongzhi K; Delbrook, Cindy; Feldman, Steven A; Fry, Terry J; Orentas, Rimas; Sabatino, Marianna; Shah, Nirali N; Steinberg, Seth M; Stroncek, Dave; Tschernia, Nick; Yuan, Constance; Zhang, Hua; Zhang, Ling; Rosenberg, Steven A; Wayne, Alan S; Mackall, Crystal L

2015-02-07

Chimeric antigen receptor (CAR) modified T cells targeting CD19 have shown activity in case series of patients with acute and chronic lymphocytic leukaemia and B-cell lymphomas, but feasibility, toxicity, and response rates of consecutively enrolled patients treated with a consistent regimen and assessed on an intention-to-treat basis have not been reported. We aimed to define feasibility, toxicity, maximum tolerated dose, response rate, and biological correlates of response in children and young adults with refractory B-cell malignancies treated with CD19-CAR T cells. This phase 1, dose-escalation trial consecutively enrolled children and young adults (aged 1-30 years) with relapsed or refractory acute lymphoblastic leukaemia or non-Hodgkin lymphoma. Autologous T cells were engineered via an 11-day manufacturing process to express a CD19-CAR incorporating an anti-CD19 single-chain variable fragment plus TCR zeta and CD28 signalling domains. All patients received fludarabine and cyclophosphamide before a single infusion of CD19-CAR T cells. Using a standard 3 + 3 design to establish the maximum tolerated dose, patients received either 1 × 10(6) CAR-transduced T cells per kg (dose 1), 3 × 10(6) CAR-transduced T cells per kg (dose 2), or the entire CAR T-cell product if sufficient numbers of cells to meet the assigned dose were not generated. After the dose-escalation phase, an expansion cohort was treated at the maximum tolerated dose. The trial is registered with ClinicalTrials.gov, number NCT01593696. Between July 2, 2012, and June 20, 2014, 21 patients (including eight who had previously undergone allogeneic haematopoietic stem-cell transplantation) were enrolled and infused with CD19-CAR T cells. 19 received the prescribed dose of CD19-CAR T cells, whereas the assigned dose concentration could not be generated for two patients (90% feasible). All patients enrolled were assessed for response. The maximum tolerated dose was defined as 1 × 10(6) CD19-CAR T cells per kg. All toxicities were fully reversible, with the most severe being grade 4 cytokine release syndrome that occurred in three (14%) of 21 patients (95% CI 3·0-36·3). The most common non-haematological grade 3 adverse events were fever (nine [43%] of 21 patients), hypokalaemia (nine [43%] of 21 patients), fever and neutropenia (eight [38%] of 21 patients), and cytokine release syndrome (three [14%) of 21 patients). CD19-CAR T cell therapy is feasible, safe, and mediates potent anti-leukaemic activity in children and young adults with chemotherapy-resistant B-precursor acute lymphoblastic leukaemia. All toxicities were reversible and prolonged B-cell aplasia did not occur. National Institutes of Health Intramural funds and St Baldrick's Foundation. Copyright © 2015 Elsevier Ltd. All rights reserved.

Electric vehicles in China: emissions and health impacts.

PubMed

Ji, Shuguang; Cherry, Christopher R; J Bechle, Matthew; Wu, Ye; Marshall, Julian D

2012-02-21

E-bikes in China are the single largest adoption of alternative fuel vehicles in history, with more than 100 million e-bikes purchased in the past decade and vehicle ownership about 2× larger for e-bikes as for conventional cars; e-car sales, too, are rapidly growing. We compare emissions (CO(2), PM(2.5), NO(X), HC) and environmental health impacts (primary PM(2.5)) from the use of conventional vehicles (CVs) and electric vehicles (EVs) in 34 major cities in China. CO(2) emissions (g km(-1)) vary and are an order of magnitude greater for e-cars (135-274) and CVs (150-180) than for e-bikes (14-27). PM(2.5) emission factors generally are lower for CVs (gasoline or diesel) than comparable EVs. However, intake fraction is often greater for CVs than for EVs because combustion emissions are generally closer to population centers for CVs (tailpipe emissions) than for EVs (power plant emissions). For most cities, the net result is that primary PM(2.5) environmental health impacts per passenger-km are greater for e-cars than for gasoline cars (3.6× on average), lower than for diesel cars (2.5× on average), and equal to diesel buses. In contrast, e-bikes yield lower environmental health impacts per passenger-km than the three CVs investigated: gasoline cars (2×), diesel cars (10×), and diesel buses (5×). Our findings highlight the importance of considering exposures, and especially the proximity of emissions to people, when evaluating environmental health impacts for EVs.

The influence of parental education and other socio-economic factors on child car seat use.

PubMed

Rok Simon, Mateja; Korošec, Aleš; Bilban, Marjan

2017-03-01

The behaviour of parents in ensuring car passenger safety for their children is associated with socio-economic (SE) status of the family; however, the influence of parental education has rarely been researched and the findings are contradictory. The aim of the study was to clarify whether parental education influences the use of a child car seat during short rides. A cross-sectional survey was carried out in outpatient clinics for children's healthcare across Slovenia. 904 parents of 3-year-old children participated in the study; the response rate was 95.9%. A self-administered questionnaire was used. A binary multiple logistic regression was applied to assess the association between parental unsafe behaviour as dependent variable, and education and other SE factors as independent variables. 14.6% of parents did not use a child car seat during short rides. Families where mother had low or college education had higher odds of the non-use of a child car seat than families where mother had a university education. Single-parent families and those who lived in areas with low or medium SE status also had higher odds of the non-use of a child car seat. Low educational attainment influences parents' behaviour regarding the non-use of a child car seat. Low parental education is not the only risk factor since some highly educated parents also have high odds of unsafe behaviour. All parents should therefore be included in individually tailored safety counselling programmes. SE inequalities could be further reduced with provision of free child car seats for eligible families.

Lenalidomide enhances the function of chimeric antigen receptor T cells against the epidermal growth factor receptor variant III by enhancing immune synapses.

PubMed

Kuramitsu, S; Ohno, M; Ohka, F; Shiina, S; Yamamichi, A; Kato, A; Tanahashi, K; Motomura, K; Kondo, G; Kurimoto, M; Senga, T; Wakabayashi, T; Natsume, A

2015-10-01

The epidermal growth factor receptor variant III (EGFRvIII) is exclusively expressed on the cell surface in ~50% of glioblastoma multiforme (GBM). This variant strongly and persistently activates the phosphatidylinositol 3-kinase-Akt signaling pathway in a ligand-independent manner resulting in enhanced tumorigenicity, cellular motility and resistance to chemoradiotherapy. Our group generated a recombinant single-chain variable fragment (scFv) antibody specific to the EGFRvIII, referred to as 3C10-scFv. In the current study, we constructed a lentiviral vector transducing the chimeric antigen receptor (CAR) that consisted of 3C10-scFv, CD3ζ, CD28 and 4-1BB (3C10-CAR). The 3C10-CAR-transduced peripheral blood mononuclear cells (PBMCs) and CD3(+) T cells specifically lysed the glioma cells that express EGFRvIII. Moreover, we demonstrated that CAR CD3(+) T cells migrated to the intracranial xenograft of GBM in the mice treated with 3C10-CAR PBMCs. An important and novel finding of our study was that a thalidomide derivative lenalidomide induced 3C10-CAR PBMC proliferation and enhanced the persistent antitumor effect of the cells in vivo. Lenalidomide also exhibited enhanced immunological synapses between the effector cells and the target cells as determined by CD11a and F-actin polymerization. Collectively, lentiviral-mediated transduction of CAR effectors targeting the EGFRvIII showed specific efficacy, and lenalidomide even intensified CAR cell therapy by enhanced formation of immunological synapses.

Functional analysis of four naturally occurring variants of human constitutive androstane receptor.

PubMed

Ikeda, Shinobu; Kurose, Kouichi; Jinno, Hideto; Sai, Kimie; Ozawa, Shogo; Hasegawa, Ryuichi; Komamura, Kazuo; Kotake, Takeshi; Morishita, Hideki; Kamakura, Shiro; Kitakaze, Masafumi; Tomoike, Hitonobu; Tamura, Tomohide; Yamamoto, Noboru; Kunitoh, Hideo; Yamada, Yasuhide; Ohe, Yuichiro; Shimada, Yasuhiro; Shirao, Kuniaki; Kubota, Kaoru; Minami, Hironobu; Ohtsu, Atsushi; Yoshida, Teruhiko; Saijo, Nagahiro; Saito, Yoshiro; Sawada, Jun-ichi

2005-01-01

The human constitutive androstane receptor (CAR, NR1I3) is a member of the orphan nuclear receptor superfamily that plays an important role in the control of drug metabolism and disposition. In this study, we sequenced all the coding exons of the NR1I3 gene for 334 Japanese subjects. We identified three novel single nucleotide polymorphisms (SNPs) that induce non-synonymous alterations of amino acids (His246Arg, Leu308Pro, and Asn323Ser) residing in the ligand-binding domain of CAR, in addition to the Val133Gly variant, which was another CAR variant identified in our previous study. We performed functional analysis of these four naturally occurring CAR variants in COS-7 cells using a CYP3A4 promoter/enhancer reporter gene that includes the CAR responsive elements. The His246Arg variant caused marked reductions in both transactivation of the reporter gene and in the response to 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO), which is a human CAR-specific agonist. The transactivation ability of the Leu308Pro variant was also significantly decreased, but its responsiveness to CITCO was not abrogated. The transactivation ability and CITCO response of the Val133Gly and Asn323Ser variants did not change as compared to the wild-type CAR. These data suggest that the His246Arg and Leu308Pro variants, especially His246Arg, may influence the expression of drug-metabolizing enzymes and transporters that are transactivated by CAR.

Assessment of Integrated Pedestrian Protection Systems with Autonomous Emergency Braking (AEB) and Passive Safety Components.

PubMed

Edwards, Mervyn; Nathanson, Andrew; Carroll, Jolyon; Wisch, Marcus; Zander, Oliver; Lubbe, Nils

2015-01-01

Autonomous emergency braking (AEB) systems fitted to cars for pedestrians have been predicted to offer substantial benefit. On this basis, consumer rating programs-for example, the European New Car Assessment Programme (Euro NCAP)-are developing rating schemes to encourage fitment of these systems. One of the questions that needs to be answered to do this fully is how the assessment of the speed reduction offered by the AEB is integrated with the current assessment of the passive safety for mitigation of pedestrian injury. Ideally, this should be done on a benefit-related basis. The objective of this research was to develop a benefit-based methodology for assessment of integrated pedestrian protection systems with AEB and passive safety components. The method should include weighting procedures to ensure that it represents injury patterns from accident data and replicates an independently estimated benefit of AEB. A methodology has been developed to calculate the expected societal cost of pedestrian injuries, assuming that all pedestrians in the target population (i.e., pedestrians impacted by the front of a passenger car) are impacted by the car being assessed, taking into account the impact speed reduction offered by the car's AEB (if fitted) and the passive safety protection offered by the car's frontal structure. For rating purposes, the cost for the assessed car is normalized by comparing it to the cost calculated for a reference car. The speed reductions measured in AEB tests are used to determine the speed at which each pedestrian in the target population will be impacted. Injury probabilities for each impact are then calculated using the results from Euro NCAP pedestrian impactor tests and injury risk curves. These injury probabilities are converted into cost using "harm"-type costs for the body regions tested. These costs are weighted and summed. Weighting factors were determined using accident data from Germany and Great Britain and an independently estimated AEB benefit. German and Great Britain versions of the methodology are available. The methodology was used to assess cars with good, average, and poor Euro NCAP pedestrian ratings, in combination with a current AEB system. The fitment of a hypothetical A-pillar airbag was also investigated. It was found that the decrease in casualty injury cost achieved by fitting an AEB system was approximately equivalent to that achieved by increasing the passive safety rating from poor to average. Because the assessment was influenced strongly by the level of head protection offered in the scuttle and windscreen area, a hypothetical A-pillar airbag showed high potential to reduce overall casualty cost. A benefit-based methodology for assessment of integrated pedestrian protection systems with AEB has been developed and tested. It uses input from AEB tests and Euro NCAP passive safety tests to give an integrated assessment of the system performance, which includes consideration of effects such as the change in head impact location caused by the impact speed reduction given by the AEB.

Hybrid femtosecond/picosecond coherent anti-Stokes Raman scattering for gas-phase temperature measurements

NASA Astrophysics Data System (ADS)

Miller, Joseph Daniel

Hybrid femtosecond/picosecond coherent anti-Stokes Raman scattering (fs/ps CARS) is employed for quantitative gas-phase temperature measurements in combustion processes and heated flows. In this approach, ultrafast 100-fs laser pulses are used to induce vibrational and rotational transitions in N2 and O2, while a third spectrally narrowed picosecond pulse is used to probe the molecular response. Temporal suppression of the nonresonant contribution and elimination of collisional effects are achieved by delay of the probe pulse, while sufficient spectral resolution is maintained for frequency-domain detection and thermometry. A theoretical framework is developed to model experimental spectra by phenomenologically describing the temporal evolution of the vibrational and rotational wavepackets as a function of temperature and pressure. Interference-free, single-shot vibrational fs/ps CARS thermometry is demonstrated at 1-kHz from 1400-2400 K in a H2-air flame, with accuracy better than 3%. A time-asymmetric exponential pulse shape is introduced to optimize nonresonant suppression with a 103 reduction at a probe delay of 0.31 ps. Low-temperature single-shot thermometry (300-700 K) with better than 1.5% accuracy is demonstrated using a fully degenerate rotational fs/ps CARS scheme, and the influence of collision energy transfer on thermometry error is quantified at atmospheric pressure. Interference-free thermometry, without nonresonant contributions and collision-induced error, is demonstrated for the first time using rotational fs/ps CARS at room temperature and pressures from 1-15 atm. Finally, the temporal and spectral resolution of fs/ps CARS is exploited for transition-resolved time-domain measurements of N2 and O2 self-broadened S-branch Raman linewidths at pressures of 1-20 atm.

The safety potential of lane departure warning systems-A descriptive real-world study of fatal lane departure passenger car crashes in Sweden.

PubMed

Sternlund, Simon

2017-05-29

Lane departure crashes account for a significant proportion of passenger car occupant fatalities and serious injuries. Utilizing real-world data involving fatal passenger car crashes in Sweden, the characteristics of lane departure crashes were identified and the safety potential of lane departure warning (LDW) systems was quantified. The material consisted of 104 in-depth studies of fatal passenger car crashes in 2010. The crashes were classified as single-vehicle (n = 48), head-on (n = 52), and overtaking (n = 4) crashes. These crash types were identified as crashes that could have potentially involved lane departure. A case-by-case analysis was carried out and lane departure crashes were identified and characterized using police reports and information collected by crash investigators at the Swedish Transport Administration; for example, inspections and photographs of the crash sites and of the involved vehicles. Lane departure crashes were separated from crashes where loss of control occurred pre-lane departure. Furthermore, loss of control post-lane departures were identified. When studying the pre-stage of lane departure without prior loss of control, crashes were categorized as unintentional drifting, intentional lane change, or evasive maneuver. Using previously published effectiveness information, the potential for LDW systems to prevent crashes was estimated. Of all crashes with passenger car occupant fatalities in Sweden in 2010, 46% (63/138) were found to relate to lane departure without prior loss of control. These crashes accounted for 61% (63/104) of all single-vehicle, head-on, and overtaking crashes. The remaining 41 crashes were due to loss of control pre-lane departure. Unintentional drifting accounted for 81% (51/63) of all lane departure crashes without prior loss of control, which corresponded to 37% (51/138) of all fatal passenger car occupant crashes. LDW systems were found to potentially prevent 33-38 of the 100 fatal head-on and single vehicle crashes. These crashes involved drifting and occurred on roads with visible lane markings, signed posted speed limits ≥70 km/h, and without rumble strips on the corresponding lane departure side. The range of potentially prevented crashes (33-38) is due to the inclusion or exclusion of crashes involving excessive speeding. In this study, approximately half (51/100) of all head-on and single-vehicle crashes were identified as being a consequence of drifting, where LDW systems had the potential to prevent the majority (33-38) of these crashes. The typical lane departure crash without prior loss of control occurred on undivided roads in rural areas with signed posted speed limits ≥70 km/h, where the center and side road markings were visible.

Limits of Generalization between Categories and Implications for Theories of Category Specificity

PubMed Central

Bukach, Cindy M.; Phillips, W. Stewart; Gauthier, Isabel

2010-01-01

Both domain-specific and expertise accounts of category specialization assume that generalization occurs within a domain but not between domains. Yet it is often difficult to define the boundaries and critical features of object domains. Differences in how categories are defined make it difficult to adjudicate between accounts of category specificity and may lead to contradictory results. For example, evidence for whether car experts recruit the fusiform face area is mixed and this inconsistency may be due to the inclusion of antique cars in one of the studies. The current study tested the generalization of expertise from modern to antique cars and found that modern car experts showed expert discrimination and holistic processing of modern cars, but not antique cars. These findings suggest that the neural specialization underlying perceptual expertise is highly specific and may not generalize to distinct subclasses, even when they share some degree of perceptual and conceptual features. PMID:20952784

3. EXTERIOR VIEW TO THE NORTHEAST OF A RAILROAD CAR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. EXTERIOR VIEW TO THE NORTHEAST OF A RAILROAD CAR ON THE TRACKS AND THE PARTS OF AN ENGINE STAND. - Nevada Test Site, Pluto Facility, Area 26, Wahmonie Flats, Cane Spring Road, Mercury, Nye County, NV

The UltraBattery-A new battery design for a new beginning in hybrid electric vehicle energy storage

NASA Astrophysics Data System (ADS)

Cooper, A.; Furakawa, J.; Lam, L.; Kellaway, M.

The UltraBattery, developed by CSIRO Energy Technology in Australia, is a hybrid energy storage device which combines an asymmetric super-capacitor and a lead-acid battery in single unit cells. This takes the best from both technologies without the need for extra, expensive electronic controls. The capacitor enhances the power and lifespan of the lead-acid battery as it acts as a buffer during high-rate discharging and charging, thus enabling it to provide and absorb charge rapidly during vehicle acceleration and braking. The initial performance of the prototype UltraBatteries was evaluated according to the US FreedomCAR targets and was shown to meet or exceed these in terms of power, available energy, cold cranking and self-discharge set for both minimum and maximum power-assist hybrid electric vehicles (HEVs). Other laboratory cycling tests showed a fourfold improvement over previous state-of-the-art lead-acid batteries under the RHOLAB test profile and better life than commercial nickel/metal hydride (NiMH) cells used in a Honda Insight when tested under the EUCAR HEV profile. As a result of this work, a set of twelve 12 V modules was built by The Furukawa Battery Co., Ltd. in Japan and were fitted into a Honda Insight instead of the NiMH battery by Provector Ltd. The battery pack was fitted with full monitoring and control capabilities and the car was tested at Millbrook Proving Ground under a General Motors road test simulation cycle for an initial target of 50 000 miles which was extended to 100 000 miles. This was completed on 15th January 2008 without any battery problems. Furthermore, the whole test was completed without the need for any conditioning or equalisation of the battery pack.

Optical skin biopsies by clinical CARS and multiphoton fluorescence/SHG tomography

NASA Astrophysics Data System (ADS)

König, K.; Breunig, H. G.; Bückle, R.; Kellner-Höfer, M.; Weinigel, M.; Büttner, E.; Sterry, W.; Lademann, J.

2011-06-01

The ultimate challenge for early diagnostics is label-free high-resolution intratissue imaging without taking physical biopsies. A novel hybrid femtosecond laser tomograph provides in vivo optical biopsies of human skin based on non-linear excitation of autofluorescence and the detection of lipids and water by CARS. Applications include skin cancer detection, biosafety tests of intradermal nanoparticles, and the testing of anti-aging products.

40 CFR Appendix Xiii to Part 86 - State Requirements Incorporated by Reference in Part 86 of the Code of Federal Regulations

Code of Federal Regulations, 2012 CFR

2012-07-01

... AND IN-USE HIGHWAY VEHICLES AND ENGINES (CONTINUED) Pt. 86, App. XIII Appendix XIII to Part 86—State...-Line Test Procedures for 1983 Through 1997 Model-Year Passenger Cars, Light-Duty Trucks and Medium-Duty...: California Assembly-Line Test Procedures for 1998 and Subsequent Model-Year Passenger Cars, Light-Duty Trucks...

40 CFR Appendix Xiii to Part 86 - State Requirements Incorporated by Reference in Part 86 of the Code of Federal Regulations

Code of Federal Regulations, 2013 CFR

2013-07-01

... AND IN-USE HIGHWAY VEHICLES AND ENGINES (CONTINUED) Pt. 86, App. XIII Appendix XIII to Part 86—State...-Line Test Procedures for 1983 Through 1997 Model-Year Passenger Cars, Light-Duty Trucks and Medium-Duty...: California Assembly-Line Test Procedures for 1998 and Subsequent Model-Year Passenger Cars, Light-Duty Trucks...

40 CFR Appendix Xiii to Part 86 - State Requirements Incorporated by Reference in Part 86 of the Code of Federal Regulations

Code of Federal Regulations, 2011 CFR

2011-07-01

... AND IN-USE HIGHWAY VEHICLES AND ENGINES (CONTINUED) Pt. 86, App. XIII Appendix XIII to Part 86—State...-Line Test Procedures for 1983 Through 1997 Model-Year Passenger Cars, Light-Duty Trucks and Medium-Duty...: California Assembly-Line Test Procedures for 1998 and Subsequent Model-Year Passenger Cars, Light-Duty Trucks...

Phase I clinical trial will test multi-targeted immunotherapy in common childhood cancer | Center for Cancer Research

Cancer.gov

Chimeric antigen receptor (CAR) T-cell immunotherapy targeting the protein CD19 has shown promise in treating acute lymphoblastic leukemia (ALL). CD22-CAR T-cell therapy has yielded similarly encouraging results, but many patients relapse after either therapy. In an upcoming phase I clinical trial, Center for Cancer Research investigators will test a new strategy—treating

A novel clinical multimodal multiphoton tomograph for AF, SHG, CARS imaging, and FLIM

NASA Astrophysics Data System (ADS)

Weinigel, Martin; Breunig, Hans Georg; König, Karsten

2014-02-01

We report on a flexible nonlinear medical tomograph with multiple miniaturized detectors for simultaneous acquisition of two-photon autofluorescence (AF), second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) images. The simultaneous visualization of the distribution of endogenous fluorophores NAD(P)H, melanin and elastin, SHG-active collagen and as well as non-fluorescent lipids within human skin in vivo is possible. Furthermore, fluorescence lifetime images (FLIM) can be generated using time-correlated single photon counting.

CARS measurement of vibrational and rotational temperature with high power laser and high speed visualization of total radiation behind hypervelocity shock waves of 5-7km/s

NASA Astrophysics Data System (ADS)

Sakurai, Kotaro; Bindu, Venigalla Hima; Niinomi, Shota; Ota, Masanori; Maeno, Kazuo

2010-09-01

Coherent Anti-Stokes Raman Spectroscopy (CARS) method is commonly used for measuring molecular structure or condition. In the aerospace technology, this method is applies to measure the temperature in thermic fluid with relatively long time duration of millisecond or sub millisecond. On the other hand, vibrational/rotational temperatures behind hypervelocity shock wave are important for heat-shield design in phase of reentry flight. The non-equilibrium flow with radiative heating from strongly shocked air ahead of the vehicles plays an important role on the heat flux to the wall surface structure as well as convective heating. In this paper CARS method is applied to measure the vibrational/rotational temperature of N2 behind hypervelocity shock wave. The strong shock wave in front of the reentering space vehicles can be experimentally realigned by free-piston, double-diaphragm shock tube with low density test gas. However CARS measurement is difficult for our experiment. Our measurement needs very short pulse which order of nanosecond and high power laser for CARS method. It is due to our measurement object is the momentary phenomena which velocity is 7km/s. In addition the observation section is low density test gas, and there is the strong background light behind the shock wave. So we employ the CARS method with high power, order of 1J/pulse, and very short pulse (10ns) laser. By using this laser the CARS signal can be acquired even in the strong radiation area. Also we simultaneously try to use the CCD camera to obtain total radiation with CARS method.

Energy-absorbing car seat designs for reducing whiplash.

PubMed

Himmetoglu, S; Acar, M; Bouazza-Marouf, K; Taylor, A J

2008-12-01

This study presents an investigation of anti-whiplash features that can be implemented in a car seat to reduce whiplash injuries in the case of a rear impact. The main emphasis is on achieving a seat design with good energy absorption properties. A biofidelic 50th percentile male multi-body human model for rear impact is developed to evaluate the performance of car seat design concepts. The model is validated using the responses of 7 volunteers from the Japanese Automobile Research Institute (JARI) sled tests, which were performed at an impact speed of 8 kph with a rigid seat and without head restraint and seatbelt. A generic multi-body car seat model is also developed to implement various seatback and recliner properties, anti-whiplash devices, and head restraints. Using the same driving posture and the rigid seat in the JARI sled tests as the basic configuration, several anti-whiplash seats are designed to allow different types of motion for the seatback and seat-pan. The anti-whiplash car seat design concepts limit neck internal motion successfully until the head-to-head restraint contact occurs and they exhibit low NIC(max) values (7 m(2)/s(2) on average). They are also effective in reducing neck compression forces and T1 forward accelerations. In principle, these car seat design concepts employ controlled recliner rotation and seat-pan displacement to limit the formation of S-shape. This is accomplished by using anti-whiplash devices that absorb the crash energy in such a way that an optimum protection is provided at different severities. The results indicate that the energy absorbing car seat design concepts all demonstrate good whiplash-reducing performances at the IIWPG standard pulse. Especially in higher severity rear impacts, two of the car seat design concepts reduce the ramping of the occupant considerably.

Test Review: E. Schopler, M. E. Van Bourgondien, G. J. Wellman, & S. R. Love "Childhood Autism Rating Scale" (2nd Ed.). Los Angeles, CA--Western Psychological Services, 2010

ERIC Educational Resources Information Center

Vaughan, Chelsea A.

2011-01-01

The author reviews Childhood Autism Rating Scale-Second Edition (CARS2), a useful tool for supporting the diagnostic process and for forming intervention recommendations once a diagnosis has been made. CARS2 consists of three rating forms designed to identify symptoms associated with autism spectrum disorders (ASD). Published in 2010, the CARS2…

East Europe Report, Economic and Industrial Affairs, Number 2425.

DTIC Science & Technology

1983-07-21

the proper tightening of turn buckles, and proper quality repairs and maintenance of car traction systems in car depots and railroad plants . The...Improve the quality of planned engine repairs in depots and repair plants . In 1982 a damaged railroad car has had to be uncoupled in...tons) were applied. The tests were conducted on the press of the G. Dimitrov ZhPZ [Railroad Plant ] in Sofia. Table 1 shows the dependence between

Estimating the drink driving attributable fraction of road traffic deaths in Mexico.

PubMed

Santoyo-Castillo, Dzoara; Pérez-Núñez, Ricardo; Borges, Guilherme; Híjar, Martha

2018-05-01

To estimate the Drink Driving Attributable Fraction (DDAF) of road traffic injury mortality in car occupants in Mexico during 2010-13. A case-control study was conducted to examine the presence of alcohol in analysed body fluids of car occupants killed in fatal crashes (cases) compared with car drivers tested in alcohol-testing checkpoints who were not involved in a fatal collision (controls). Two data sets were used for the period 2010-13: the forensic module of the Epidemiological Surveillance System on Addictions that included car occupants killed in a collision (cases) and a data set from alcohol-testing at police checkpoints available for matching municipalities (controls). Mexico. The analysed study sample included 1718 car occupants killed in a traffic collision and 80 656 drivers tested at alcohol police checkpoints, all from 10 municipalities. Unadjusted and adjusted odds ratios (OR) of presence of alcohol in body fluids were obtained stratified by sex and age groups and the interaction with these two variables were assessed. The ORs were used to calculate the DDAF. It was estimated that 19.5% of car occupants' deaths due to road traffic injuries were attributable to alcohol consumption [95% confidence interval (CI) = 19.1-19.9]. The adjusted OR of presence of alcohol was 6.84 (95% CI = 6.06-7.71) overall. For males it was 7.21 (95% CI = 6.35-8.18) and for females it was 4.45 (95% CI = 3.01-6.60). The ORs were similar across younger age bands (10-19 years: 9.61, 95% CI = 6.72-13.73; 20-29 years: 7.70, 95% CI = 6.28-9.4; and 30-49 years: 7.21, 95% CI = 5.98-8.70); and lower but still elevated among older people (50+ years: 3.19, 95% CI = 2.19-4.65). An estimated 19.5% of car occupant deaths in Mexico may have been caused by alcohol in 2010-13. © 2017 Society for the Study of Addiction.

An Alternative Approach to ``Measuring Horsepower and Torque Curves of a Car''

NASA Astrophysics Data System (ADS)

Graney, Chris M.

2005-09-01

The article in the September 2003 issue of TPT by John Ross Buschert of Goshen College entitled "Measuring Horsepower and Torque Curves of a Car" was excellent. I attained similar results using existing automobile test data. Automobile performance tests done by magazines such as Road & Track are a treasure trove of good-quality physics data. Performance tests often contain all the data needed to replicate Professor Buschert's analysis of the power and torque output of automobile engines.

Nitroaromatic carcinogens in diesel soot: a review of laboratory findings.

PubMed Central

Wei, E T; Shu, H P

1983-01-01

The automobile industry plans to increase production of diesel-powered passenger cars because diesel engines provide better fuel economy than conventional gasoline engines. Diesel engines, however, produce more soot, and increased use of diesel cars will result in more discharge of diesel soot into the atmosphere. Recently, a new class of chemicals, called nitroaromatic compounds, have been identified in chemical extracts of diesel soot. Some of these nitroaromatic compounds produce mutations when tested in in vitro bacterial and mammalian cell assays, and cancer when tested in animals. Here, we review the relevance of these new laboratory findings to current deliberations over emission standards for particles from diesel cars. PMID:6192732

Interior car noise created by textured pavement surfaces : final report.

DOT National Transportation Integrated Search

1975-01-01

Because of widespread concern about the effect of textured pavement surfaces on interior car noise, sound pressure levels (SPL) were measured inside a test vehicle as it traversed 21 pavements with various textures. A linear regression analysis run o...

Universal Artificial Antigen Presenting Cells to Selectively Propagate T Cells Expressing Chimeric Antigen Receptor Independent of Specificity

PubMed Central

Rushworth, David; Jena, Bipulendu; Olivares, Simon; Maiti, Sourindra; Briggs, Neima; Somanchi, Srinivas; Dai, Jianliang; Lee, Dean; Cooper, Laurence J. N.

2014-01-01

T cells genetically modified to stably express immunoreceptors are being assessed for therapeutic potential in clinical trials. T cells expressing a chimeric antigen receptor (CAR) are endowed with a new specificity to target tumor-associated antigen (TAA) independent of major histocompatibility complex. Our approach to non-viral gene transfer in T cells uses ex vivo numeric expansion of CAR+ T cells on irradiated artificial antigen presenting cells (aAPC) bearing the targeted TAA. The requirement for aAPC to express a desired TAA limits the human application of CARs with multiple specificities when selective expansion through co-culture with feeder cells is sought. As an alternative to expressing individual TAAs on aAPC, we expressed one ligand that could activate CAR+ T cells for sustained proliferation independent of specificity. We expressed a CAR ligand (designated CARL) that binds the conserved IgG4 extracellular domain of CAR and demonstrated CARL+ aAPC propagate CAR+ T cells of multiple specificities. CARL avoids technical issues and costs associated with deploying clinical-grade aAPC for each TAA targeted by a given CAR. Employing CARL enables one aAPC to numerically expand all CAR+ T cells containing the IgG4 domain, and simplifies expansion, testing, and clinical translation of CAR+ T cells of any specificity. PMID:24714354

CD22-targeted CAR T cells induce remission in B-ALL that is naive or resistant to CD19-targeted CAR immunotherapy.

PubMed

Fry, Terry J; Shah, Nirali N; Orentas, Rimas J; Stetler-Stevenson, Maryalice; Yuan, Constance M; Ramakrishna, Sneha; Wolters, Pamela; Martin, Staci; Delbrook, Cindy; Yates, Bonnie; Shalabi, Haneen; Fountaine, Thomas J; Shern, Jack F; Majzner, Robbie G; Stroncek, David F; Sabatino, Marianna; Feng, Yang; Dimitrov, Dimiter S; Zhang, Ling; Nguyen, Sang; Qin, Haiying; Dropulic, Boro; Lee, Daniel W; Mackall, Crystal L

2018-01-01

Chimeric antigen receptor (CAR) T cells targeting CD19 mediate potent effects in relapsed and/or refractory pre-B cell acute lymphoblastic leukemia (B-ALL), but antigen loss is a frequent cause of resistance to CD19-targeted immunotherapy. CD22 is also expressed in most cases of B-ALL and is usually retained following CD19 loss. We report results from a phase 1 trial testing a new CD22-targeted CAR (CD22-CAR) in 21 children and adults, including 17 who were previously treated with CD19-directed immunotherapy. Dose-dependent antileukemic activity was observed, with complete remission obtained in 73% (11/15) of patients receiving ≥1 × 10 6 CD22-CAR T cells per kg body weight, including 5 of 5 patients with CD19 dim or CD19 - B-ALL. Median remission duration was 6 months. Relapses were associated with diminished CD22 site density that likely permitted CD22 + cell escape from killing by CD22-CAR T cells. These results are the first to establish the clinical activity of a CD22-CAR in B-ALL, including leukemia resistant to anti-CD19 immunotherapy, demonstrating potency against B-ALL comparable to that of CD19-CAR at biologically active doses. Our results also highlight the critical role played by antigen density in regulating CAR function.

Analysis of multiple tank car releases in train accidents.

PubMed

Liu, Xiang; Liu, Chang; Hong, Yili

2017-10-01

There are annually over two million carloads of hazardous materials transported by rail in the United States. The American railroads use large blocks of tank cars to transport petroleum crude oil and other flammable liquids from production to consumption sites. Being different from roadway transport of hazardous materials, a train accident can potentially result in the derailment and release of multiple tank cars, which may result in significant consequences. The prior literature predominantly assumes that the occurrence of multiple tank car releases in a train accident is a series of independent Bernoulli processes, and thus uses the binomial distribution to estimate the total number of tank car releases given the number of tank cars derailing or damaged. This paper shows that the traditional binomial model can incorrectly estimate multiple tank car release probability by magnitudes in certain circumstances, thereby significantly affecting railroad safety and risk analysis. To bridge this knowledge gap, this paper proposes a novel, alternative Correlated Binomial (CB) model that accounts for the possible correlations of multiple tank car releases in the same train. We test three distinct correlation structures in the CB model, and find that they all outperform the conventional binomial model based on empirical tank car accident data. The analysis shows that considering tank car release correlations would result in a significantly improved fit of the empirical data than otherwise. Consequently, it is prudent to consider alternative modeling techniques when analyzing the probability of multiple tank car releases in railroad accidents. Copyright © 2017 Elsevier Ltd. All rights reserved.

Integrating Norm Activation Model and Theory of Planned Behavior to Understand Sustainable Transport Behavior: Evidence from China.

PubMed

Liu, Yuwei; Sheng, Hong; Mundorf, Norbert; Redding, Colleen; Ye, Yinjiao

2017-12-18

With increasing urbanization in China, many cities are facing serious environmental problems due to continuous and substantial increase in automobile transportation. It is becoming imperative to examine effective ways to reduce individual automobile use to facilitate sustainable transportation behavior. Empirical, theory-based research on sustainable transportation in China is limited. In this research, we propose an integrated model based on the norm activation model and the theory of planned behavior by combining normative and rational factors to predict individuals' intention to reduce car use. Data from a survey of 600 car drivers in China's three metropolitan areas was used to test the proposed model and hypotheses. Results showed that three variables, perceived norm of car-transport reduction, attitude towards reduction, and perceived behavior control over car-transport reduction, significantly affected the intention to reduce car-transport. Personal norms mediated the relationship between awareness of consequences of car-transport, ascription of responsibility of car-transport, perceived subjective norm for car-transport reduction, and intention to reduce car-transport. The results of this research not only contribute to theory development in the area of sustainable transportation behavior, but also provide a theoretical frame of reference for relevant policy-makers in urban transport management.

Integrating Norm Activation Model and Theory of Planned Behavior to Understand Sustainable Transport Behavior: Evidence from China

PubMed Central

Liu, Yuwei; Sheng, Hong; Mundorf, Norbert; Redding, Colleen

2017-01-01

With increasing urbanization in China, many cities are facing serious environmental problems due to continuous and substantial increase in automobile transportation. It is becoming imperative to examine effective ways to reduce individual automobile use to facilitate sustainable transportation behavior. Empirical, theory-based research on sustainable transportation in China is limited. In this research, we propose an integrated model based on the norm activation model and the theory of planned behavior by combining normative and rational factors to predict individuals’ intention to reduce car use. Data from a survey of 600 car drivers in China’s three metropolitan areas was used to test the proposed model and hypotheses. Results showed that three variables, perceived norm of car-transport reduction, attitude towards reduction, and perceived behavior control over car-transport reduction, significantly affected the intention to reduce car-transport. Personal norms mediated the relationship between awareness of consequences of car-transport, ascription of responsibility of car-transport, perceived subjective norm for car-transport reduction, and intention to reduce car-transport. The results of this research not only contribute to theory development in the area of sustainable transportation behavior, but also provide a theoretical frame of reference for relevant policy-makers in urban transport management. PMID:29258245

Airborne Infectious Agents and Other Pollutants in Automobiles for Domestic Use: Potential Health Impacts and Approaches to Risk Mitigation

PubMed Central

Zargar, Bahram; Rubino, Joseph R.

2016-01-01

The world total of passenger cars is expected to go from the current one billion to >2.5 billion by 2050. Cars for domestic use account for ~74% of the world's yearly production of motorized vehicles. In North America, ~80% of the commuters use their own car with another 5.6% travelling as passengers. With the current life-expectancy of 78.6 years, the average North American spends 4.3 years driving a car! This equates to driving 101 minutes/day with a lifetime driving distance of nearly 1.3 million km inside the confined and often shared space of the car with exposure to a mix of potentially harmful pathogens, allergens, endotoxins, particulates, and volatile organics. Such risks may increase in proportion to the unprecedented upsurge in the numbers of family cars globally. Though new technologies may reduce the levels of air pollution from car exhausts and other sources, they are unlikely to impact our in-car exposure to pathogens. Can commercial in-car air decontamination devices reduce the risk from airborne infections and other pollutants? We lack scientifically rigorous protocols to verify the claims of such devices. Here we discuss the essentials of a customized aerobiology facility and test protocols to assess such devices under field-relevant conditions. PMID:28042302

Airborne Infectious Agents and Other Pollutants in Automobiles for Domestic Use: Potential Health Impacts and Approaches to Risk Mitigation.

PubMed

Sattar, Syed A; Wright, Kathryn E; Zargar, Bahram; Rubino, Joseph R; Ijaz, M Khalid

2016-01-01

The world total of passenger cars is expected to go from the current one billion to >2.5 billion by 2050. Cars for domestic use account for ~74% of the world's yearly production of motorized vehicles. In North America, ~80% of the commuters use their own car with another 5.6% travelling as passengers. With the current life-expectancy of 78.6 years, the average North American spends 4.3 years driving a car! This equates to driving 101 minutes/day with a lifetime driving distance of nearly 1.3 million km inside the confined and often shared space of the car with exposure to a mix of potentially harmful pathogens, allergens, endotoxins, particulates, and volatile organics. Such risks may increase in proportion to the unprecedented upsurge in the numbers of family cars globally. Though new technologies may reduce the levels of air pollution from car exhausts and other sources, they are unlikely to impact our in-car exposure to pathogens. Can commercial in-car air decontamination devices reduce the risk from airborne infections and other pollutants? We lack scientifically rigorous protocols to verify the claims of such devices. Here we discuss the essentials of a customized aerobiology facility and test protocols to assess such devices under field-relevant conditions.

Automotive Insulation

NASA Technical Reports Server (NTRS)

1997-01-01

Under a Space Act Agreement between Boeing North America and BSR Products, Space Shuttle Thermal Protection System (TPS) materials are now used to insulate race cars. BSR has created special TPS blanket insulation kits for use on autos that take part in NASCAR events, and other race cars through its nationwide catalog distribution system. Temperatures inside a race car's cockpit can soar to a sweltering 140 to 160 degrees, with the extreme heat coming through the engine firewall, transmission tunnel, and floor. It is common for NASCAR drivers to endure blisters and burns due to the excessive heat. Tests on a car insulated with the TPS material showed a temperature drop of some 50 degrees in the driver's cockpit. BSR-TPS Products, Inc. now manufactures insulation kits for distribution to race car teams around the world.

Diagnostic Imaging in Flames with Instantaneous Planar Coherent Raman Spectroscopy.

PubMed

Bohlin, A; Kliewer, C J

2014-04-03

Spatial mapping of temperature and molecular species concentrations is vitally important in studies of gaseous chemically reacting flows. Temperature marks the evolution of heat release and energy transfer, while species concentration gradients provide critical information on mixing and chemical reaction. Coherent anti-Stokes Raman spectroscopy (CARS) was pioneered in measurements of such processes almost 40 years ago and is authoritative in terms of the accuracy and precision it may provide. While a reacting flow is fully characterized in three-dimensional space, a limitation of CARS has been its applicability as a point-wise measurement technique, motivating advancement toward CARS imaging, and attempts have been made considering one-dimensional probing. Here, we report development of two-dimensional CARS, with the first diagnostics of a planar field in a combusting flow within a single laser pulse, resulting in measured isotherms ranging from 450 K up to typical hydrocarbon flame temperatures of about 2000 K with chemical mapping of O2 and N2.

Modelling and analysis of the crush zone of a typical Australian passenger train

NASA Astrophysics Data System (ADS)

Sun, Y. Q.; Cole, C.; Dhanasekar, M.; Thambiratnam, D. P.

2012-07-01

In this paper, a three-dimensional nonlinear rigid body model has been developed for the investigation of the crashworthiness of a passenger train using the multibody dynamics approach. This model refers to a typical design of passenger cars and train constructs commonly used in Australia. The high-energy and low-energy crush zones of the cars and the train constructs are assumed and the data are explicitly provided in the paper. The crash scenario is limited to the train colliding on to a fixed barrier symmetrically. The simulations of a single car show that this initial design is only applicable for the crash speed of 35 km/h or lower. For higher speeds (e.g. 140 km/h), the crush lengths or crush forces or both the crush zone elements will have to be enlarged. It is generally better to increase the crush length than the crush force in order to retain the low levels of the longitudinal deceleration of the passenger cars.

Analysis of work zone rear-end crash risk for different vehicle-following patterns.

PubMed

Weng, Jinxian; Meng, Qiang; Yan, Xuedong

2014-11-01

This study evaluates rear-end crash risk associated with work zone operations for four different vehicle-following patterns: car-car, car-truck, truck-car and truck-truck. The deceleration rate to avoid the crash (DRAC) is adopted to measure work zone rear-end crash risk. Results show that the car-truck following pattern has the largest rear-end crash risk, followed by truck-truck, truck-car and car-car patterns. This implies that it is more likely for a car which is following a truck to be involved in a rear-end crash accident. The statistical test results further confirm that rear-end crash risk is statistically different between any two of the four patterns. We therefore develop a rear-end crash risk model for each vehicle-following pattern in order to examine the relationship between rear-end crash risk and its influencing factors, including lane position, the heavy vehicle percentage, lane traffic flow and work intensity which can be characterized by the number of lane reductions, the number of workers and the amount of equipment at the work zone site. The model results show that, for each pattern, there will be a greater rear-end crash risk in the following situations: (i) heavy work intensity; (ii) the lane adjacent to work zone; (iii) a higher proportion of heavy vehicles and (iv) greater traffic flow. However, the effects of these factors on rear-end crash risk are found to vary according to the vehicle-following patterns. Compared with the car-car pattern, lane position has less effect on rear-end crash risk in the car-truck pattern. The effect of work intensity on rear-end crash risk is also reduced in the truck-car pattern. Copyright © 2014 Elsevier Ltd. All rights reserved.

Experimental cross-correlation nitrogen Q-branch CARS thermometry in a spark ignition engine

NASA Astrophysics Data System (ADS)

Lockett, R. D.; Ball, D.; Robertson, G. N.

2013-07-01

A purely experimental technique was employed to derive temperatures from nitrogen Q-branch Coherent Anti-Stokes Raman Scattering (CARS) spectra, obtained in a high pressure, high temperature environment (spark ignition Otto engine). This was in order to obviate any errors arising from deficiencies in the spectral scaling laws which are commonly used to represent nitrogen Q-branch CARS spectra at high pressure. The spectra obtained in the engine were compared with spectra obtained in a calibrated high pressure, high temperature cell, using direct cross-correlation in place of the minimisation of sums of squares of residuals. The technique is demonstrated through the measurement of air temperature as a function of crankshaft angle inside the cylinder of a motored single-cylinder Ricardo E6 research engine, followed by the measurement of fuel-air mixture temperatures obtained during the compression stroke in a knocking Ricardo E6 engine. A standard CARS programme (SANDIA's CARSFIT) was employed to calibrate the altered non-resonant background contribution to the CARS spectra that was caused by the alteration to the mole fraction of nitrogen in the unburned fuel-air mixture. The compression temperature profiles were extrapolated in order to predict the auto-ignition temperatures.

Enhancing older driver safety: A driving survey and evaluation of the CarFit program.

PubMed

Gaines, Jean M; Burke, Kasey L; Marx, Katherine A; Wagner, Mary; Parrish, John M

2011-10-01

To evaluate CarFit, an educational program designed to promote optimal alignment of driver with vehicle. A driving activity survey was sent to 727 randomly selected participants living in retirement communities. Drivers (n=195) were assigned randomly to CarFit intervention (n=83, M age=78.1) or Comparison (n=112, M age=79.6) groups. After 6months, participants completed a post-test of driving activity and CarFit recommendations. Nonconsenting drivers were older and participated in fewer driving activities. CarFit participation was moderate (71%) with 86% of the participants receiving recommendations. 60% followed the recommendations at the 6-month re-evaluation). The CarFit (67.6%) and Comparison (59.3%) groups reported at least one type of self-regulation of driving activity at baseline. There was no significant change in the driving behaviors at the six-month follow-up. CarFit was able to detect addressable opportunities that may contribute to the safety of older drivers. CarFit recommendations may need stronger reinforcement in order to be enacted by a participant. Copyright © 2011 National Safety Council and Elsevier Ltd. All rights reserved.

Effectiveness of electronic stability control on single-vehicle accidents.

PubMed

Lyckegaard, Allan; Hels, Tove; Bernhoft, Inger Marie

2015-01-01

This study aims at evaluating the effectiveness of electronic stability control (ESC) on single-vehicle injury accidents while controlling for a number of confounders influencing the accident risk. Using police-registered injury accidents from 2004 to 2011 in Denmark with cars manufactured in the period 1998 to 2011 and the principle of induced exposure, 2 measures of the effectiveness of ESC were calculated: The crude odds ratio and the adjusted odds ratio, the latter by means of logistic regression. The logistic regression controlled for a number of confounding factors, of which the following were significant. For the driver: Age, gender, driving experience, valid driving license, and seat belt use. For the vehicle: Year of registration, weight, and ESC. For the accident surroundings: Visibility, light, and location. Finally, for the road: Speed limit, surface, and section characteristics. The present study calculated the crude odds ratio for ESC-equipped cars of getting in a single-vehicle injury accident as 0.40 (95% confidence interval [CI], 0.34-0.47) and the adjusted odds ratio as 0.69 (95% CI, 0.54-0.88). No difference was found in the effectiveness of ESC across the injury severity categories (slight, severe, and fatal). In line with previous results, this study concludes that ESC reduces the risk for single-vehicle injury accidents by 31% when controlling for various confounding factors related to the driver, the car, and the accident surroundings. Furthermore, it is concluded that it is important to control for human factors (at a minimum age and gender) in analyses where evaluations of this type are performed.

[Steady car engine noise does not affect the cognitive abilities of sleep apnea syndrome patients].

PubMed

Mayoralas, L R; Barbé, F; Muñoz, A; Agustí, A G N

2003-09-01

Traffic accidents are more frequent for sleep apnea syndrome (SAS) patients than in the population at large. The mechanisms that underlie this observation are poorly defined. Our working hypothesis was that in SAS patients the steady noise of a car engine might alter cognitive capacities that may be involved in driving, thus increasing the risk of traffic accidents. To test this hypothesis we designed a prospective randomized controlled trial. Eighteen SAS patients (apnea-hypopnea index [SEM] 62 [6] h1) and 18 healthy controls were studied. All the participants were evaluated in random order both in basal conditions and after exposure to the steady noise of a car engine recorded on a compact disc. Their level of vigilance was evaluated (Steer-Clear) as well as their reaction time (PVT 192). Attention, coordination, and memory were measured using the following tests: Wechsler Memory Scale (digit span), the Wechsler Intelligence Scale (digit symbol), and Lezack's Trail Making tests A and B. The SAS patients were slightly younger than the control group (mean 50 [7] vs 57 [11] years, respectively; P=.05). The patients showed a lower level of vigilance than the controls both in basal and engine noise conditions (P<.05). No differences between groups were found for the other variables studied. Exposure to steady car engine noise had no effect on the tests of either group. In conclusion, the results of our study do not support the hypothesis that steady car engine noise significantly alters the cognitive ability of SAS patients.

76 FR 30239 - Volkswagen Group of America, Inc., Grant of Petition for Decision of Inconsequential Noncompliance

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-24

...,292 2009 MY Audi A6 and S6 model passenger cars, 2010 MY Audi A6, S6, A5, A5 Cabrio, S5, S5 Cabrio, A4 and S4 passenger cars, and 2010 MY Audi Q5 MPV's with indirect TPMS manufactured between October 17... test of a 2009 MY Audi A6 model passenger car to FMVSS No. 138 requirements. On June 3, 2009, OVSC...

Heat transfer augmentation of a car radiator using nanofluids

NASA Astrophysics Data System (ADS)

Hussein, Adnan M.; Bakar, R. A.; Kadirgama, K.; Sharma, K. V.

2014-05-01

The car radiator heat transfer enhancement by using TiO2 and SiO2 nanoparticles dispersed in water as a base fluid was studied experimentally. The test rig is setup as a car radiator with tubes and container. The range of Reynolds number and volume fraction are (250-1,750) and (1.0-2.5 %) respectively. Results showed that the heat transfer increases with increasing of nanofluid volume fraction. The experimental data is agreed with other investigator.