Sample records for size shape morphology

  1. Recent advances in understanding nuclear size and shape

    PubMed Central

    Mukherjee, Richik N.; Chen, Pan; Levy, Daniel L.

    2016-01-01

    ABSTRACT Size and shape are important aspects of nuclear structure. While normal cells maintain nuclear size within a defined range, altered nuclear size and shape are associated with a variety of diseases. It is unknown if altered nuclear morphology contributes to pathology, and answering this question requires a better understanding of the mechanisms that control nuclear size and shape. In this review, we discuss recent advances in our understanding of the mechanisms that regulate nuclear morphology, focusing on nucleocytoplasmic transport, nuclear lamins, the endoplasmic reticulum, the cell cycle, and potential links between nuclear size and size regulation of other organelles. We then discuss the functional significance of nuclear morphology in the context of early embryonic development. Looking toward the future, we review new experimental approaches that promise to provide new insights into mechanisms of nuclear size control, in particular microfluidic-based technologies, and discuss how altered nuclear morphology might impact chromatin organization and physiology of diseased cells. PMID:26963026

  2. Monogenean anchor morphometry: systematic value, phylogenetic signal, and evolution

    PubMed Central

    Soo, Oi Yoon Michelle; Tan, Wooi Boon; Lim, Lee Hong Susan

    2016-01-01

    Background. Anchors are one of the important attachment appendages for monogenean parasites. Common descent and evolutionary processes have left their mark on anchor morphometry, in the form of patterns of shape and size variation useful for systematic and evolutionary studies. When combined with morphological and molecular data, analysis of anchor morphometry can potentially answer a wide range of biological questions. Materials and Methods. We used data from anchor morphometry, body size and morphology of 13 Ligophorus (Monogenea: Ancyrocephalidae) species infecting two marine mugilid (Teleostei: Mugilidae) fish hosts: Moolgarda buchanani (Bleeker) and Liza subviridis (Valenciennes) from Malaysia. Anchor shape and size data (n = 530) were generated using methods of geometric morphometrics. We used 28S rRNA, 18S rRNA, and ITS1 sequence data to infer a maximum likelihood phylogeny. We discriminated species using principal component and cluster analysis of shape data. Adams’s Kmult was used to detect phylogenetic signal in anchor shape. Phylogeny-correlated size and shape changes were investigated using continuous character mapping and directional statistics, respectively. We assessed morphological constraints in anchor morphometry using phylogenetic regression of anchor shape against body size and anchor size. Anchor morphological integration was studied using partial least squares method. The association between copulatory organ morphology and anchor shape and size in phylomorphospace was used to test the Rohde-Hobbs hypothesis. We created monogeneaGM, a new R package that integrates analyses of monogenean anchor geometric morphometric data with morphological and phylogenetic data. Results. We discriminated 12 of the 13 Ligophorus species using anchor shape data. Significant phylogenetic signal was detected in anchor shape. Thus, we discovered new morphological characters based on anchor shaft shape, the length between the inner root point and the outer root point, and the length between the inner root point and the dent point. The species on M. buchanani evolved larger, more robust anchors; those on L. subviridis evolved smaller, more delicate anchors. Anchor shape and size were significantly correlated, suggesting constraints in anchor evolution. Tight integration between the root and the point compartments within anchors confirms the anchor as a single, fully integrated module. The correlation between male copulatory organ morphology and size with anchor shape was consistent with predictions from the Rohde-Hobbs hypothesis. Conclusions. Monogenean anchors are tightly integrated structures, and their shape variation correlates strongly with phylogeny, thus underscoring their value for systematic and evolutionary biology studies. Our MonogeneaGM R package provides tools for researchers to mine biological insights from geometric morphometric data of speciose monogenean genera. PMID:26966649

  3. The morphology of human hyoid bone in relation to sex, age and body proportions.

    PubMed

    Urbanová, P; Hejna, P; Zátopková, L; Šafr, M

    2013-06-01

    Morphological aspects of the human hyoid bone are, like many other skeletal elements in human body, greatly affected by individual's sex, age and body proportions. Still, the known sex-dependent bimodality of a number of body size characteristics overshadows the true within-group patterns. Given the ambiguity of the causal effects of age, sex and body size upon hyoid morphology the present study puts the relationship between shape of human hyoid bone and body proportions (height and weight) under scrutiny of a morphological study. Using 211 hyoid bones and landmark-based methods of geometric morphometrics, it was shown that the size of hyoid bones correlated positively with measured body dimensions but showed no correlation if the individual's sex was controlled for. For shape variables, our results revealed that hyoid morphology is clearly related to body size as expressed in terms of the height and weight. Yet, the hyoid shape was shown to result primarily from the sex-related bimodal distribution of studied body size descriptors which, in the case of the height-dependent model, exhibited opposite trends for males and females. Apart from the global hyoid shape given by spatial arrangements of the greater horns, body size dependency was translated into size and position of the hyoid body. None of the body size characters had any impact on hyoid asymmetry. Ultimately, sexually dimorphic variation was revealed for age-dependent changes in both size and shape of hyoid bones as male hyoids tend to be more susceptible to modifications with age than female bones. Copyright © 2013 Elsevier GmbH. All rights reserved.

  4. Morphologically and size uniform monodisperse particles and their shape-directed self-assembly

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Collins, Joshua E.; Bell, Howard Y.; Ye, Xingchen

    2017-09-12

    Monodisperse particles having: a single pure crystalline phase of a rare earth-containing lattice, a uniform three-dimensional size, and a uniform polyhedral morphology are disclosed. Due to their uniform size and shape, the monodisperse particles self assemble into superlattices. The particles may be luminescent particles such as down-converting phosphor particles and up-converting phosphors. The monodisperse particles of the invention have a rare earth-containing lattice which in one embodiment may be an yttrium-containing lattice or in another may be a lanthanide-containing lattice. The monodisperse particles may have different optical properties based on their composition, their size, and/or their morphology (or shape). Alsomore » disclosed is a combination of at least two types of monodisperse particles, where each type is a plurality of monodisperse particles having a single pure crystalline phase of a rare earth-containing lattice, a uniform three-dimensional size, and a uniform polyhedral morphology; and where the types of monodisperse particles differ from one another by composition, by size, or by morphology. In a preferred embodiment, the types of monodisperse particles have the same composition but different morphologies. Methods of making and methods of using the monodisperse particles are disclosed.« less

  5. Morphologically and size uniform monodisperse particles and their shape-directed self-assembly

    DOEpatents

    Collins, Joshua E.; Bell, Howard Y.; Ye, Xingchen; Murray, Christopher Bruce

    2015-11-17

    Monodisperse particles having: a single pure crystalline phase of a rare earth-containing lattice, a uniform three-dimensional size, and a uniform polyhedral morphology are disclosed. Due to their uniform size and shape, the monodisperse particles self assemble into superlattices. The particles may be luminescent particles such as down-converting phosphor particles and up-converting phosphors. The monodisperse particles of the invention have a rare earth-containing lattice which in one embodiment may be an yttrium-containing lattice or in another may be a lanthanide-containing lattice. The monodisperse particles may have different optical properties based on their composition, their size, and/or their morphology (or shape). Also disclosed is a combination of at least two types of monodisperse particles, where each type is a plurality of monodisperse particles having a single pure crystalline phase of a rare earth-containing lattice, a uniform three-dimensional size, and a uniform polyhedral morphology; and where the types of monodisperse particles differ from one another by composition, by size, or by morphology. In a preferred embodiment, the types of monodisperse particles have the same composition but different morphologies. Methods of making and methods of using the monodisperse particles are disclosed.

  6. [Achene morphology cluster analysis of Taraxacum F. H. Wigg. from northeast China and molecule systematics evidence determined by SRAP].

    PubMed

    Li, Hai-juan; Zhao, Xin; Jia, Qing-fei; Li, Tian-lai; Ning, Wei

    2012-08-01

    The achenes morphological and micro-morphological characteristics of six species of genus Taraxacum from northeastern China as well as SRAP cluster analysis were observed for their classification evidences. The achenes were observed by microscope and EPMA. Cluster analysis was given on the basis of the size, shape, cone proportion, color and surface sculpture of achenes. The Taraxacum inter-species achene shape characteristic difference is obvious, particularly spinulose distribution and size, achene color and achene size; with the Taraxacum plant achene shape the cluster method T. antungense Kitag. and the T. urbanum Kitag. should combine for the identical kind; the achene morphology cluster analysis and the SRAP tagged molecule systematics's cluster result retrieves in the table with "the Chinese flora". The class group to divide the result is consistent. Taraxacum plant achene shape characteristic stable conservative, may carry on the inter-species division and the sibship analysis according to the achene shape characteristic combination difference; the achene morphology cluster analysis as well as the SRAP tagged molecule systematics confirmation support dandelion classification result of "the Chinese flora".

  7. Ecological and evolutionary influences on body size and shape in the Galápagos marine iguana (Amblyrhynchus cristatus).

    PubMed

    Chiari, Ylenia; Glaberman, Scott; Tarroso, Pedro; Caccone, Adalgisa; Claude, Julien

    2016-07-01

    Oceanic islands are often inhabited by endemic species that have undergone substantial morphological evolutionary change due to processes of multiple colonizations from various source populations, dispersal, and local adaptation. Galápagos marine iguanas are an example of an island endemic exhibiting high morphological diversity, including substantial body size variation among populations and sexes, but the causes and magnitude of this variation are not well understood. We obtained morphological measurements from marine iguanas throughout their distribution range. These data were combined with genetic and local environmental data from each population to investigate the effects of evolutionary history and environmental conditions on body size and shape variation and sexual dimorphism. Our results indicate that body size and shape are highly variable among populations. Sea surface temperature and island perimeter, but not evolutionary history as depicted by phylogeographic patterns in this species, explain variation in body size among populations. Conversely, evolutionary history, but not environmental parameters or island size, was found to influence variation in body shape among populations. Finally, in all populations except one, we found strong sexual dimorphism in body size and shape in which males are larger, with higher heads than females, while females have longer heads than males. Differences among populations suggest that plasticity and/or genetic adaptation may shape body size and shape variation in marine iguanas. This study will help target future investigations to address the contribution of plasticity versus genetic adaptation on size and shape variation in marine iguanas.

  8. A continuous morphological approach to study the evolution of pollen in a phylogenetic context: An example with the order Myrtales.

    PubMed

    Kriebel, Ricardo; Khabbazian, Mohammad; Sytsma, Kenneth J

    2017-01-01

    The study of pollen morphology has historically allowed evolutionary biologists to assess phylogenetic relationships among Angiosperms, as well as to better understand the fossil record. During this process, pollen has mainly been studied by discretizing some of its main characteristics such as size, shape, and exine ornamentation. One large plant clade in which pollen has been used this way for phylogenetic inference and character mapping is the order Myrtales, composed by the small families Alzateaceae, Crypteroniaceae, and Penaeaceae (collectively the "CAP clade"), as well as the large families Combretaceae, Lythraceae, Melastomataceae, Myrtaceae, Onagraceae and Vochysiaceae. In this study, we present a novel way to study pollen evolution by using quantitative size and shape variables. We use morphometric and morphospace methods to evaluate pollen change in the order Myrtales using a time-calibrated, supermatrix phylogeny. We then test for conservatism, divergence, and morphological convergence of pollen and for correlation between the latitudinal gradient and pollen size and shape. To obtain an estimate of shape, Myrtales pollen images were extracted from the literature, and their outlines analyzed using elliptic Fourier methods. Shape and size variables were then analyzed in a phylogenetic framework under an Ornstein-Uhlenbeck process to test for shifts in size and shape during the evolutionary history of Myrtales. Few shifts in Myrtales pollen morphology were found which indicates morphological conservatism. Heterocolpate, small pollen is ancestral with largest pollen in Onagraceae. Convergent shifts in shape but not size occurred in Myrtaceae and Onagraceae and are correlated to shifts in latitude and biogeography. A quantitative approach was applied for the first time to examine pollen evolution across a large time scale. Using phylogenetic based morphometrics and an OU process, hypotheses of pollen size and shape were tested across Myrtales. Convergent pollen shifts and position in the latitudinal gradient support the selective role of harmomegathy, the mechanism by which pollen grains accommodate their volume in response to water loss.

  9. A continuous morphological approach to study the evolution of pollen in a phylogenetic context: An example with the order Myrtales

    PubMed Central

    Khabbazian, Mohammad; Sytsma, Kenneth J.

    2017-01-01

    The study of pollen morphology has historically allowed evolutionary biologists to assess phylogenetic relationships among Angiosperms, as well as to better understand the fossil record. During this process, pollen has mainly been studied by discretizing some of its main characteristics such as size, shape, and exine ornamentation. One large plant clade in which pollen has been used this way for phylogenetic inference and character mapping is the order Myrtales, composed by the small families Alzateaceae, Crypteroniaceae, and Penaeaceae (collectively the “CAP clade”), as well as the large families Combretaceae, Lythraceae, Melastomataceae, Myrtaceae, Onagraceae and Vochysiaceae. In this study, we present a novel way to study pollen evolution by using quantitative size and shape variables. We use morphometric and morphospace methods to evaluate pollen change in the order Myrtales using a time-calibrated, supermatrix phylogeny. We then test for conservatism, divergence, and morphological convergence of pollen and for correlation between the latitudinal gradient and pollen size and shape. To obtain an estimate of shape, Myrtales pollen images were extracted from the literature, and their outlines analyzed using elliptic Fourier methods. Shape and size variables were then analyzed in a phylogenetic framework under an Ornstein-Uhlenbeck process to test for shifts in size and shape during the evolutionary history of Myrtales. Few shifts in Myrtales pollen morphology were found which indicates morphological conservatism. Heterocolpate, small pollen is ancestral with largest pollen in Onagraceae. Convergent shifts in shape but not size occurred in Myrtaceae and Onagraceae and are correlated to shifts in latitude and biogeography. A quantitative approach was applied for the first time to examine pollen evolution across a large time scale. Using phylogenetic based morphometrics and an OU process, hypotheses of pollen size and shape were tested across Myrtales. Convergent pollen shifts and position in the latitudinal gradient support the selective role of harmomegathy, the mechanism by which pollen grains accommodate their volume in response to water loss. PMID:29211730

  10. Size and shape-dependent cytotoxicity profile of gold nanoparticles for biomedical applications.

    PubMed

    Woźniak, Anna; Malankowska, Anna; Nowaczyk, Grzegorz; Grześkowiak, Bartosz F; Tuśnio, Karol; Słomski, Ryszard; Zaleska-Medynska, Adriana; Jurga, Stefan

    2017-06-01

    Metallic nanoparticles, in particular gold nanoparticles (AuNPs), offer a wide spectrum of applications in biomedicine. A crucial issue is their cytotoxicity, which depends greatly on various factors, including morphology of nanoparticles. Because metallic nanoparticles have an effect on cell membrane integrity, their shape and size may affect the viability of cells, due to their different geometries as well as physical and chemical interactions with cell membranes. Variations in the size and shape of gold nanoparticles may indicate particular nanoparticle morphologies that provide strong cytotoxicity effects. Synthesis of different sized and shaped bare AuNPs was performed with spherical (~ 10 nm), nanoflowers (~ 370 nm), nanorods (~ 41 nm), nanoprisms (~ 160 nm) and nanostars (~ 240 nm) morphologies. These nanostructures were characterized and interacting with cancer (HeLa) and normal (HEK293T) cell lines and cell viability tests were performed by WST-1 tests and fluorescent live/dead cell imaging experiments. It was shown that various shapes and sizes of gold nanostructures may affect the viability of the cells. Gold nanospheres and nanorods proved to be more toxic than star, flower and prism gold nanostructures. This may be attributed to their small size and aggregation process. This is the first report concerning a comparison of cytotoxic profile in vitro with a wide spectrum of bare AuNPs morphology. The findings show their possible use in biomedical applications.

  11. Monodisperse Block Copolymer Particles with Controllable Size, Shape, and Nanostructure

    NASA Astrophysics Data System (ADS)

    Shin, Jae Man; Kim, Yongjoo; Kim, Bumjoon; PNEL Team

    Shape-anisotropic particles are important class of novel colloidal building block for their functionality is more strongly governed by their shape, size and nanostructure compared to conventional spherical particles. Recently, facile strategy for producing non-spherical polymeric particles by interfacial engineering received significant attention. However, achieving uniform size distribution of particles together with controlled shape and nanostructure has not been achieved. Here, we introduce versatile system for producing monodisperse BCP particles with controlled size, shape and morphology. Polystyrene-b-polybutadiene (PS-b-PB) self-assembled to either onion-like or striped ellipsoid particle, where final structure is governed by amount of adsorbed sodium dodecyl sulfate (SDS) surfactant at the particle/surrounding interface. Further control of molecular weight and particle size enabled fine-tuning of aspect ratio of ellipsoid particle. Underlying physics of free energy for morphology formation and entropic penalty associated with bending BCP chains strongly affects particle structure and specification.

  12. Elliptical Fourier descriptors of outline and morphological analysis in caudal view of foramen magnum of the tropical raccoon (Procyon cancrivorus) (Linnaeus, 1758).

    PubMed

    Samuel, O M; Casanova, P M; Olopade, J O

    2018-03-01

    To evaluate sexual-size dimorphism and attempt at categorization of inter-individual shapes of foramen magnum outlines using Fourier descriptors which allow for shape outline evaluations with a resultant specimen character definition. Individual characterization and quantification of foramen magnum shapes in direct caudal view based on elliptical Fourier technique was applied to 46 tropical raccoon skulls (26 females, 20 males). Incremental number of harmonics demonstrates morphological contributions of such descriptors with their relations to specific anatomical constructions established. The initial harmonics (1st to 3rd) described the general foramen shapes while the second (4th to 12th) demonstrated fine morphological details. Sexual-size dimorphism was observed in females (87.1%) and 91.7% in males, normalization of size produces 75% in females and 83% in males. With respect to foramen magnum dimorphism analysis, the result obtained through elliptic Fourier analysis was comparatively better in detail information of outline contours than earlier classical methods. The first four effective principal components defined 70.63% of its shape properties while the rest (22.51%) constituted fine details of morphology. Both size and shape seems important in sexual dimorphisms in this species, this investigation suggest clinical implications, taxonomic and anthropologic perspectives in foramen characterization magnum characterization and further postulates an increased possibility of volume reduction cerebellar protrusion, ontogenic magnum shape irregularities in the sample population with neurologic consequences especially among females. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  13. Ontogenetic scaling of caudal fin shape in Squalus acanthias (Chondrichthyes, Elasmobranchii): a geometric morphometric analysis with implications for caudal fin functional morphology.

    PubMed

    Reiss, Katie L; Bonnan, Matthew F

    2010-07-01

    The shark heterocercal caudal fin and its contribution to locomotion are of interest to biologists and paleontologists. Current hydrodynamic data show that the stiff dorsal lobe leads the ventral lobe, both lobes of the tail are synchronized during propulsion, and tail shape reflects its overall locomotor function. Given the difficulties surrounding the analysis of shark caudal fins in vivo, little is known about changes in tail shape related to ontogeny and sex in sharks. A quantifiable analysis of caudal fin shape may provide an acceptable proxy for inferring gross functional morphology where direct testing is difficult or impossible. We examined ontogenetic and sex-related shape changes in the caudal fins of 115 Squalus acanthias museum specimens, to test the hypothesis that significant shape changes in the caudal fin shape occur with increasing size and between the sexes. Using linear and geometric morphometrics, we examined caudal shape changes within the context of current hydrodynamic models. We found no statistically significant linear or shape difference between sexes, and near-isometric scaling trends for caudal dimensions. These results suggest that lift and thrust increase linearly with size and caudal span. Thin-plate splines results showed a significant allometric shape change associated with size and caudal span: the dorsal lobe elongates and narrows, whereas the ventral lobe broadens and expands ventrally. Our data suggest a combination of caudal fin morphology with other body morphology aspects, would refine, and better elucidate the hydrodynamic factors (if any) that underlie the significant shape changes we report here for S. acanthias.

  14. Analysis of morphological variability and heritability in the head of the Argentine Black and White Tegu (Salvator merianae): undisturbed vs. disturbed environments.

    PubMed

    Imhoff, Carolina; Giri, Federico; Siroski, Pablo; Amavet, Patricia

    2018-04-01

    The heterogeneity of biotic and abiotic factors influencing fitness produce selective pressures that promote local adaptation and divergence among different populations of the same species. In order for adaptations to be maintained through evolutionary time, heritable genetic variation controlling the expression of the morphological features under selection is necessary. Here we compare morphological shape variability and size of the cephalic region of Salvator merianae specimens from undisturbed environments to those of individuals from disturbed environments, and estimated heritability for shape and size using geometric morphometric and quantitative genetics tools. The results of these analyzes indicated that there are statistically significant differences in shape and size between populations from the two environments. Possibly, one of the main determinants of cephalic shape and size is adaptation to the characteristics of the environment and to the trophic niche. Individuals from disturbed environments have a cephalic region with less shape variation and also have a larger centroid size when compared to individuals from undisturbed environments. The high heritability values obtained for shape and size in dorsal view and right side view indicate that these phenotypic characters have a great capacity to respond to the selection pressures to which they are subjected. Data obtained here could be used as an important tool when establishing guidelines for plans for the sustainable use and conservation of S. merianae and other species living in disturbed areas. Copyright © 2018 Elsevier GmbH. All rights reserved.

  15. Morphologically controlled synthesis of ferric oxide nano/micro particles and their catalytic application in dry and wet media: a new approach.

    PubMed

    Janjua, Muhammad Ramzan Saeed Ashraf; Jamil, Saba; Jahan, Nazish; Khan, Shanza Rauf; Mirza, Saima

    2017-05-31

    Morphologically controlled synthesis of ferric oxide nano/micro particles has been carried out by using solvothermal route. Structural characterization displays that the predominant morphologies are porous hollow spheres, microspheres, micro rectangular platelets, octahedral and irregular shaped particles. It is also observed that solvent has significant effect on morphology such as shape and size of the particles. All the morphologies obtained by using different solvents are nearly uniform with narrow size distribution range. The values of full width at half maxima (FWHM) of all the products were calculated to compare their size distribution. The FWHM value varies with size of the particles for example small size particles show polydispersity whereas large size particles have shown monodispersity. The size of particles increases with decrease in polarity of the solvent whereas their shape changes from spherical to rectangular/irregular with decrease in polarity of the solvent. The catalytic activities of all the products were investigated for both dry and wet processes such as thermal decomposition of ammonium per chlorate (AP) and reduction of 4-nitrophenol in aqueous media. The results indicate that each product has a tendency to act as a catalyst. The porous hollow spheres decrease the thermal decomposition temperature of AP by 140 °C and octahedral Fe 3 O 4 particles decrease the decomposition temperature by 30 °C. The value of apparent rate constant (k app ) of reduction of 4-NP has also been calculated.

  16. Otolith morphology varies between populations, sexes and male alternative reproductive tactics in a vocal toadfish Porichthys notatus.

    PubMed

    Bose, A P H; Adragna, J B; Balshine, S

    2017-01-01

    In this study, the morphology of sagittal otoliths of the plainfin midshipman fish Porichthys notatus was compared between populations, sexes and male alternative reproductive phenotypes (known as 'type I males or guarders' and 'type II males or sneakers'). Sagitta size increased with P. notatus size and changes in shape were also detected with increasing body size. Porichthys notatus sagittae begin as simple rounded structures, but then elongate as they grow and take on a more triangular and complex shape with several prominent notches and indentations along the dorsal and caudal edges. Moreover, the sagittae of the two geographically and genetically distinct populations of P. notatus (northern and southern) differed in shape. Porichthys notatus from the north possessed taller sagittae with deeper caudal indentations compared to P. notatus from the south. Sagitta shape also differed between females and males of the conventional guarder tactic. Furthermore, guarder males had smaller sagittae for their body size than did sneaker males or females. These differences in sagittal otolith morphology are discussed in relation to ecological and life history differences between the sexes and male tactics of this species. This is the first study to investigate teleost otolith morphology from the perspective of alternative reproductive tactics. © 2016 The Fisheries Society of the British Isles.

  17. Evolutionary morphology in shape and size of haptoral anchors in 14 Ligophorus spp. (Monogenea: Dactylogyridae).

    PubMed

    Rodríguez-González, Abril; Sarabeev, Volodimir; Balbuena, Juan Antonio

    2017-01-01

    The search for phylogenetic signal in morphological traits using geometric morphometrics represents a powerful approach to estimate the relative weights of convergence and shared evolutionary history in shaping organismal form. We assessed phylogenetic signal in the form of ventral and dorsal haptoral anchors of 14 species of Ligophorus occurring on grey mullets (Osteichthyes: Mugilidae) from the Mediterranean, the Black Sea and the Sea of Azov. The phylogenetic relationships among these species were mapped onto the morphospaces of shape and size of dorsal and ventral anchors and two different tests were applied to establish whether the spatial positions in the morphospace were dictated by chance. Overall significant phylogenetic signal was found in the data. Allometric effects on anchor shape were moderate or non-significant in the case of evolutionary allometry. Relatively phylogenetically distant species occurring on the same host differed markedly in anchor morphology indicating little influence of host species on anchor form. Our results suggest that common descent and shared evolutionary history play a major role in determining the shape and, to a lesser degree in the size of haptoral anchors in Ligophorus spp. The present approach allowed tracing paths of morphological evolution in anchor shape. Species with narrow anchors and long shafts were associated predominately with Liza saliens. This morphology was considered to be ancestral relative to anchors of species occurring on Liza haematocheila and M. cephalus possessing shorter shafts and longer roots. Evidence for phylogenetic signal was more compelling for the ventral anchors, than for the dorsal ones, which could reflect different functional roles in attachment to the gills. Although phylogeny and homoplasy may act differently in other monogeneans, the present study delivers a common framework to address effectively the relationships among morphology, phylogeny and other traits, such as host specificity or niche occupancy.

  18. Effect of Pore Size, Morphology and Orientation on the Bulk Stiffness of a Porous Ti35Nb4Sn Alloy

    NASA Astrophysics Data System (ADS)

    Torres-Sanchez, Carmen; McLaughlin, John; Bonallo, Ross

    2018-04-01

    The metal foams of a titanium alloy were designed to study porosity as well as pore size and shape independently. These were manufactured using a powder metallurgy/space-holder technique that allowed a fine control of the pore size and morphology; and then characterized and tested against well-established models to predict a relationship between porosity, pore size and shape, and bulk stiffness. Among the typically used correlations, existing power-law models were found to be the best fit for the prediction of macropore morphology against compressive elastic moduli, outperforming other models such as exponential, polynomial or binomial. Other traditional models such as linear ones required of updated coefficients to become relevant to metal porous sintered macrostructures. The new coefficients reported in this study contribute toward a design tool that allows the tailoring of mechanical properties through porosity macrostructure. The results show that, for the same porosity range, pore shape and orientation have a significant effect on mechanical performance and that they can be predicted. Conversely, pore size has only a mild impact on bulk stiffness.

  19. Neandertal talus bones from El Sidrón site (Asturias, Spain): A 3D geometric morphometrics analysis.

    PubMed

    Rosas, Antonio; Ferrando, Anabel; Bastir, Markus; García-Tabernero, Antonio; Estalrrich, Almudena; Huguet, Rosa; García-Martínez, Daniel; Pastor, Juan Francisco; de la Rasilla, Marco

    2017-10-01

    The El Sidrón tali sample is assessed in an evolutionary framework. We aim to explore the relationship between Neandertal talus morphology and body size/shape. We test the hypothesis 1: talar Neandertal traits are influenced by body size, and the hypothesis 2: shape variables independent of body size correspond to inherited primitive features. We quantify 35 landmarks through 3D geometric morphometrics techniques to describe H. neanderthalensis-H. sapiens shape variation, by Mean Shape Comparisons, Principal Component, Phenetic Clusters, Minimum spanning tree analyses and partial least square and regression of talus shape on body variables. Shape variation correlated to body size is compared to Neandertals-Modern Humans (MH) evolutionary shape variation. The Neandertal sample is compared to early hominins. Neandertal talus presents trochlear hypertrophy, a larger equality of trochlear rims, a shorter neck, a more expanded head, curvature and an anterior location of the medial malleolar facet, an expanded and projected lateral malleolar facet and laterally expanded posterior calcaneal facet compared to MH. The Neandertal talocrural joint morphology is influenced by body size. The other Neandertal talus traits do not co-vary with it or not follow the same co-variation pattern as MH. Besides, the trochlear hypertrophy, the trochlear rims equality and the short neck could be inherited primitive features; the medial malleolar facet morphology could be an inherited primitive feature or a secondarily primitive trait; and the calcaneal posterior facet would be an autapomorphic feature of the Neandertal lineage. © 2017 Wiley Periodicals, Inc.

  20. Rare ecomorphological convergence on a complex adaptive landscape: Body size and diet mediate evolution of jaw shape in squirrels (Sciuridae).

    PubMed

    Zelditch, Miriam Leah; Ye, Ji; Mitchell, Jonathan S; Swiderski, Donald L

    2017-03-01

    Convergence is widely regarded as compelling evidence for adaptation, often being portrayed as evidence that phenotypic outcomes are predictable from ecology, overriding contingencies of history. However, repeated outcomes may be very rare unless adaptive landscapes are simple, structured by strong ecological and functional constraints. One such constraint may be a limitation on body size because performance often scales with size, allowing species to adapt to challenging functions by modifying only size. When size is constrained, species might adapt by changing shape; convergent shapes may therefore be common when size is limiting and functions are challenging. We examine the roles of size and diet as determinants of jaw shape in Sciuridae. As expected, size and diet have significant interdependent effects on jaw shape and ecomorphological convergence is rare, typically involving demanding diets and limiting sizes. More surprising is morphological without ecological convergence, which is equally common between and within dietary classes. Those cases, like rare ecomorphological convergence, may be consequences of evolving on an adaptive landscape shaped by many-to-many relationships between ecology and function, many-to-one relationships between form and performance, and one-to-many relationships between functionally versatile morphologies and ecology. On complex adaptive landscapes, ecological selection can yield different outcomes. © 2017 The Author(s). Evolution © 2017 The Society for the Study of Evolution.

  1. Evolutionary morphology in shape and size of haptoral anchors in 14 Ligophorus spp. (Monogenea: Dactylogyridae)

    PubMed Central

    Rodríguez-González, Abril; Sarabeev, Volodimir; Balbuena, Juan Antonio

    2017-01-01

    The search for phylogenetic signal in morphological traits using geometric morphometrics represents a powerful approach to estimate the relative weights of convergence and shared evolutionary history in shaping organismal form. We assessed phylogenetic signal in the form of ventral and dorsal haptoral anchors of 14 species of Ligophorus occurring on grey mullets (Osteichthyes: Mugilidae) from the Mediterranean, the Black Sea and the Sea of Azov. The phylogenetic relationships among these species were mapped onto the morphospaces of shape and size of dorsal and ventral anchors and two different tests were applied to establish whether the spatial positions in the morphospace were dictated by chance. Overall significant phylogenetic signal was found in the data. Allometric effects on anchor shape were moderate or non-significant in the case of evolutionary allometry. Relatively phylogenetically distant species occurring on the same host differed markedly in anchor morphology indicating little influence of host species on anchor form. Our results suggest that common descent and shared evolutionary history play a major role in determining the shape and, to a lesser degree in the size of haptoral anchors in Ligophorus spp. The present approach allowed tracing paths of morphological evolution in anchor shape. Species with narrow anchors and long shafts were associated predominately with Liza saliens. This morphology was considered to be ancestral relative to anchors of species occurring on Liza haematocheila and M. cephalus possessing shorter shafts and longer roots. Evidence for phylogenetic signal was more compelling for the ventral anchors, than for the dorsal ones, which could reflect different functional roles in attachment to the gills. Although phylogeny and homoplasy may act differently in other monogeneans, the present study delivers a common framework to address effectively the relationships among morphology, phylogeny and other traits, such as host specificity or niche occupancy. PMID:28542570

  2. Morphological changes in ultrafast laser ablation plumes with varying spot size.

    PubMed

    Harilal, S S; Diwakar, P K; Polek, M P; Phillips, M C

    2015-06-15

    We investigated the role of spot size on plume morphology during ultrafast laser ablation of metal targets. Our results show that the spatial features of fs LA plumes are strongly dependent on the focal spot size. Two-dimensional self-emission images showed that the shape of the ultrafast laser ablation plumes changes from spherical to cylindrical with an increasing spot size from 100 to 600 μm. The changes in plume morphology and internal structures are related to ion emission dynamics from the plasma, where broader angular ion distribution and faster ions are noticed for the smallest spot size used. The present results clearly show that the morphological changes in the plume with spot size are independent of laser pulse width.

  3. Shape, form, function and Leishmania pathogenicity: from textbook descriptions to biological understanding

    PubMed Central

    Gull, Keith

    2017-01-01

    The shape and form of protozoan parasites are inextricably linked to their pathogenicity. The evolutionary pressure associated with establishing and maintaining an infection and transmission to vector or host has shaped parasite morphology. However, there is not a ‘one size fits all’ morphological solution to these different pressures, and parasites exhibit a range of different morphologies, reflecting the diversity of their complex life cycles. In this review, we will focus on the shape and form of Leishmania spp., a group of very successful protozoan parasites that cause a range of diseases from self-healing cutaneous leishmaniasis to visceral leishmaniasis, which is fatal if left untreated. PMID:28903998

  4. Morphological and mechanical changes in juvenile red-eared slider turtle (Trachemys scripta elegans) shells during ontogeny.

    PubMed

    Fish, Jennifer F; Stayton, Charles T

    2014-04-01

    Turtles experience numerous modifications in the morphological, physiological, and mechanical characteristics of their shells through ontogeny. Although a general picture is available of the nature of these modifications, few quantitative studies have been conducted on changes in turtle shell shape through ontogeny, and none on changes in strength or rigidity. This study investigates the morphological and mechanical changes that juvenile Trachemys scripta elegans undergo as they increase in size. Morphology and shell rigidity were quantified in a sample of 36 alcohol-preserved juvenile Trachemys scripta elegans. Morphometric information was used to create finite element models of all specimens. These models were used to assess the mechanical behavior of the shells under various loading conditions. Overall, we find that turtles experience complementary changes in size, shape, deformability, and relative strength as they grow. As turtles age their shells become larger, more elongate, relatively flatter, and more rigid. These changes are associated with decreases in relative (size independent) strength, even though the shells of larger turtles are stronger in an absolute sense. Decreased deformability is primarily due to changes in the size of the animals. Residual variation in deformability cannot be explained by changes in shell shape. This variation is more likely due to changes in the degree of connectedness of the skeletal elements in the turtle's shells, along with changes in the thickness and degree of mineralization of shell bone. We suggest that the mechanical implications of shell size, shape, and deformability may have a large impact on survivorship and development in members of this species as they mature. Copyright © 2013 Wiley Periodicals, Inc.

  5. Ecogeographical Variation in Skull Shape of South-American Canids: Abiotic or Biotic Processes?

    PubMed

    de Moura Bubadué, Jamile; Cáceres, Nilton; Dos Santos Carvalho, Renan; Meloro, Carlo

    Species morphological changes can be mutually influenced by environmental or biotic factors, such as competition. South American canids represent a quite recent radiation of taxa that evolved forms very disparate in phenotype, ecology and behaviour. Today, in the central part of South America there is one dominant large species (the maned wolf, Chrysocyon brachyurus ) that directly influence sympatric smaller taxa via interspecific killing. Further south, three species of similar sized foxes ( Lycalopex spp.) share the same habitats. Such unique combination of taxa and geographic distribution makes South American dogs an ideal group to test for the simultaneous impact of climate and competition on phenotypic variation. Using geometric morphometrics, we quantified skull size and shape of 431 specimens belonging to the eight extant South American canid species: Atelocynus microtis , Cerdocyon thous , Ch. brachyurus , Lycalopex culpaeus , L. griseus , L. gymnocercus , L. vetulus and Speothos venaticus . South American canids are significantly different in both skull size and shape. The hypercarnivorous bush dog is mostly distinct in shape from all the other taxa while a degree of overlap in shape-but not size-occurs between species of the genus Lycalopex . Both climate and competition impacts interspecific morphological variation. We identified climatic adaptations as the main driving force of diversification for the South American canids. Competition has a lower degree of impact on their skull morphology although it might have played a role in the past, when canid community was richer in morphotypes.

  6. Behavior of osteoblast-like cells on calcium-deficient hydroxyapatite ceramics composed of particles with different shapes and sizes.

    PubMed

    Kamitakahara, Masanobu; Uno, Yuika; Ioku, Koji

    2014-01-01

    In designing the biomaterials, it is important to control their surface morphologies, because they affect the interactions between the materials and cells. We previously reported that porous calcium-deficient hydroxyapatite (HA) ceramics composed of rod-like particles had advantages over sintered porous HA ceramics; however, the effects of the surface morphology of calcium-deficient HA ceramics on cell behavior have remained unclear. Using a hydrothermal process, we successfully prepared porous calcium-deficient HA ceramics with different surface morphologies, composed of plate-like particles of 200-300, 500-800 nm, or 2-3 μm in width and rod-like particles of 1 or 3-5 μm in width, respectively. The effects of these surface morphologies on the behavior of osteoblast-like cells were examined. Although the numbers of cells adhered to the ceramic specimens did not differ significantly among the specimens, the proliferation rates of cells on the ceramics decreased with decreasing particle size. Our results reveal that controlling the surface morphology that is governed by particle shape and size is important for designing porous calcium-deficient HA ceramics.

  7. Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): a geometric morphometric approach.

    PubMed

    Rodríguez-González, Abril; Míguez-Lozano, Raúl; Llopis-Belenguer, Cristina; Balbuena, Juan Antonio

    2015-04-01

    Evaluating phenotypic plasticity in attachment organs of parasites can provide information on the capacity to colonise new hosts and illuminate evolutionary processes driving host specificity. We analysed the variability in shape and size of the dorsal and ventral anchors of Ligophorus cephali from Mugil cephalus by means of geometric morphometrics and multivariate statistics. We also assessed the morphological integration between anchors and between the roots and points in order to gain insight into their functional morphology. Dorsal and ventral anchors showed a similar gradient of overall shape variation, but the amount of localised changes was much higher in the former. Statistical models describing variations in shape and size revealed clear differences between anchors. The dorsal anchor/bar complex seems more mobile than the ventral one in Ligophorus, and these differences may reflect different functional roles in attachment to the gills. The lower residual variation associated with the ventral anchor models suggests a tighter control of their shape and size, perhaps because these anchors seem to be responsible for firmer attachment and their size and shape would allow more effective responses to characteristics of the microenvironment within the individual host. Despite these putative functional differences, the high level of morphological integration indicates a concerted action between anchors. In addition, we found a slight, although significant, morphological integration between roots and points in both anchors, which suggests that a large fraction of the observed phenotypic variation does not compromise the functional role of anchors as levers. Given the low level of genetic variation in our sample, it is likely that much of the morphological variation reflects host-driven plastic responses. This supports the hypothesis of monogenean specificity through host-switching and rapid speciation. The present study demonstrates the potential of geometric morphometrics to provide new and previously unexplored insights into the functional morphology of attachment and evolutionary processes of host-parasite coevolution. Copyright © 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

  8. Morphological changes in ultrafast laser ablation plumes with varying spot size

    DOE PAGES

    Harilal, S. S.; Diwakar, P. K.; Polek, M. P.; ...

    2015-06-04

    We investigated the role of spot size on plume morphology during ultrafast laser ablation of metal targets. Our results show that the spatial features of fs LA plumes are strongly dependent on the focal spot size. Two-dimensional self-emission images showed that the shape of the ultrafast laser ablation plumes changes from spherical to cylindrical with an increasing spot size from 100 to 600 μm. The changes in plume morphology and internal structures are related to ion emission dynamics from the plasma, where broader angular ion distribution and faster ions are noticed for the smallest spot size used. The present resultsmore » clearly show that the morphological changes in the plume with spot size are independent of laser pulse width.« less

  9. ShapeRotator: An R tool for standardized rigid rotations of articulated three-dimensional structures with application for geometric morphometrics.

    PubMed

    Vidal-García, Marta; Bandara, Lashi; Keogh, J Scott

    2018-05-01

    The quantification of complex morphological patterns typically involves comprehensive shape and size analyses, usually obtained by gathering morphological data from all the structures that capture the phenotypic diversity of an organism or object. Articulated structures are a critical component of overall phenotypic diversity, but data gathered from these structures are difficult to incorporate into modern analyses because of the complexities associated with jointly quantifying 3D shape in multiple structures. While there are existing methods for analyzing shape variation in articulated structures in two-dimensional (2D) space, these methods do not work in 3D, a rapidly growing area of capability and research. Here, we describe a simple geometric rigid rotation approach that removes the effect of random translation and rotation, enabling the morphological analysis of 3D articulated structures. Our method is based on Cartesian coordinates in 3D space, so it can be applied to any morphometric problem that also uses 3D coordinates (e.g., spherical harmonics). We demonstrate the method by applying it to a landmark-based dataset for analyzing shape variation using geometric morphometrics. We have developed an R tool (ShapeRotator) so that the method can be easily implemented in the commonly used R package geomorph and MorphoJ software. This method will be a valuable tool for 3D morphological analyses in articulated structures by allowing an exhaustive examination of shape and size diversity.

  10. Temporal Trends in Vertebral Size and Shape from Medieval to Modern-Day

    PubMed Central

    Junno, Juho-Antti; Niskanen, Markku; Nieminen, Miika T.; Maijanen, Heli; Niinimäki, Jaakko; Bloigu, Risto; Tuukkanen, Juha

    2009-01-01

    Human lumbar vertebrae support the weight of the upper body. Loads lifted and carried by the upper extremities cause significant loading stress to the vertebral bodies. It is well established that trauma-induced vertebral fractures are common especially among elderly people. The aim of this study was to investigate the morphological factors that could have affected the prevalence of trauma-related vertebral fractures from medieval times to the present day. To determine if morphological differences existed in the size and shape of the vertebral body between medieval times and the present day, the vertebral body size and shape was measured from the 4th lumbar vertebra using magnetic resonance imaging (MRI) and standard osteometric calipers. The modern samples consisted of modern Finns and the medieval samples were from archaeological collections in Sweden and Britain. The results show that the shape and size of the 4th lumbar vertebra has changed significantly from medieval times in a way that markedly affects the biomechanical characteristics of the lumbar vertebral column. These changes may have influenced the incidence of trauma- induced spinal fractures in modern populations. PMID:19279681

  11. Evolutionary developmental genetics of fruit morphological variation within the Solanaceae

    PubMed Central

    Wang, Li; Li, Jing; Zhao, Jing; He, Chaoying

    2015-01-01

    Morphological variations of fruits such as shape and size, and color are a result of adaptive evolution. The evolution of morphological novelties is particularly intriguing. An understanding of these evolutionary processes calls for the elucidation of the developmental and genetic mechanisms that result in particular fruit morphological characteristics, which determine seed dispersal. The genetic and developmental basis for fruit morphological variation was established at a microevolutionary time scale. Here, we summarize the progress on the evolutionary developmental genetics of fruit size, shape and color in the Solanaceae. Studies suggest that the recruitment of a pre-existing gene and subsequent modification of its interaction and regulatory networks are frequently involved in the evolution of morphological diversity. The basic mechanisms underlying changes in plant morphology are alterations in gene expression and/or gene function. We also deliberate on the future direction in evolutionary developmental genetics of fruit morphological variation such as fruit type. These studies will provide insights into plant developmental processes and will help to improve the productivity and fruit quality of crops. PMID:25918515

  12. The 'temporal effect' in hominids: Reinvestigating the nature of support for a chimp-human clade in bone morphology.

    PubMed

    Pearson, Alannah; Groves, Colin; Cardini, Andrea

    2015-11-01

    In 2004, an analysis by Lockwood and colleagues of hard-tissue morphology, using geometric morphometrics on the temporal bone, succeeded in recovering the correct phylogeny of living hominids without resorting to potentially problematic methods for transforming continuous shape variables into meristic characters. That work has increased hope that by using modern analytical methods and phylogenetically informative anatomical data we might one day be able to accurately infer the relationships of hominins, including the closest extinct relatives of modern humans. In the present study, using 3D virtually generated models of the hominid temporal bone and a larger suite of geometric morphometric and comparative techniques, we have re-examined the evidence for a Pan-Homo clade. Despite differences in samples, as well as the type of raw data, the effect of measurement error (and especially landmark digitization by a different operator), but also a broader perspective brought in by our diverse set of approaches, our reanalysis largely supports Lockwood and colleagues' original results. However, by focusing not only mainly on shape (as in the original 2004 analysis) but also on size and 'size-corrected' (non-allometric) shape, we demonstrate that the strong phylogenetic signal in the temporal bone is largely related to similarities in size. Thus, with this study, we are not suggesting the use of a single 'character', such as size, for phylogenetic inference, but we do challenge the common view that shape, with its highly complex and multivariate nature, is necessarily more phylogenetically informative than size and that actually size and size-related shape variation (i.e., allometry) confound phylogenetic inference based on morphology. This perspective may in fact be less generalizable than often believed. Thus, while we confirm the original findings by Lockwood et al., we provide a deep reinterpretation of their nature and potential implications for hominid phylogenetics and we show how crucial it is not to overlook size in geometric morphometric analyses. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Nanocontainers made of Various Materials with Tunable Shape and Size

    NASA Astrophysics Data System (ADS)

    Zhao, Xianglong; Meng, Guowen; Han, Fangming; Li, Xiangdong; Chen, Bensong; Xu, Qiaoling; Zhu, Xiaoguang; Chu, Zhaoqin; Kong, Mingguang; Huang, Qing

    2013-07-01

    Nanocontainers have great potentials in targeted drug delivery and nanospace-confined reactions. However, the previous synthetic approaches exhibited limited control over the morphology, size and materials of the nanocontainers, which are crucial in practical applications. Here, we present a synthetic approach to multi-segment linear-shaped nanopores with pre-designed morphologies inside anodic aluminium oxide (AAO), by tailoring the anodizing duration after a rational increase of the applied anodizing voltage and the number of voltage increase during Al foil anodization. Then, we achieve nanocontainers with designed morphologies, such as nanofunnels, nanobottles, nano-separating-funnels and nanodroppers, with tunable sizes and diverse materials of carbon, silicon, germanium, hafnium oxide, silica and nickel/carbon magnetic composite, by depositing a thin layer of materials on the inner walls of the pre-designed AAO nanopores. The strategy has far-reaching implications in the designing and large-scale fabrication of nanocontainers, opening up new opportunities in nanotechnology applications.

  14. Nanocontainers made of Various Materials with Tunable Shape and Size

    PubMed Central

    Zhao, Xianglong; Meng, Guowen; Han, Fangming; Li, Xiangdong; Chen, Bensong; Xu, Qiaoling; Zhu, Xiaoguang; Chu, Zhaoqin; Kong, Mingguang; Huang, Qing

    2013-01-01

    Nanocontainers have great potentials in targeted drug delivery and nanospace-confined reactions. However, the previous synthetic approaches exhibited limited control over the morphology, size and materials of the nanocontainers, which are crucial in practical applications. Here, we present a synthetic approach to multi-segment linear-shaped nanopores with pre-designed morphologies inside anodic aluminium oxide (AAO), by tailoring the anodizing duration after a rational increase of the applied anodizing voltage and the number of voltage increase during Al foil anodization. Then, we achieve nanocontainers with designed morphologies, such as nanofunnels, nanobottles, nano-separating-funnels and nanodroppers, with tunable sizes and diverse materials of carbon, silicon, germanium, hafnium oxide, silica and nickel/carbon magnetic composite, by depositing a thin layer of materials on the inner walls of the pre-designed AAO nanopores. The strategy has far-reaching implications in the designing and large-scale fabrication of nanocontainers, opening up new opportunities in nanotechnology applications. PMID:23867836

  15. Beyond the sniffer: frontal sinuses in Carnivora.

    PubMed

    Curtis, Abigail A; Van Valkenburgh, Blaire

    2014-11-01

    Paranasal sinuses are some of the most poorly understood features of mammalian cranial anatomy. They are highly variable in presence and form among species, but their function is not well understood. The best-supported explanations for the function of sinuses is that they opportunistically fill mechanically unnecessary space, but that in some cases, sinuses in combination with the configuration of the frontal bone may improve skull performance by increasing skull strength and dissipating stresses more evenly. We used CT technology to investigate patterns in frontal sinus size and shape disparity among three families of carnivores: Canidae, Felidae, and Hyaenidae. We provide some of the first quantitative data on sinus morphology for these three families, and employ a novel method to quantify the relationship between three-dimensional sinus shape and skull shape. As expected, frontal sinus size and shape were more strongly correlated with frontal bone size and shape than with the morphology of the skull as a whole. However, sinus morphology was also related to allometric differences among families that are linked to biomechanical function. Our results support the hypothesis that frontal sinuses most often opportunistically fill space that is mechanically unnecessary, and they can facilitate cranial shape changes that reduce stress during feeding. Moreover, we suggest that the ability to form frontal sinuses allows species to modify skull function without compromising the performance of more functionally constrained regions such as the nasal chamber (heat/water conservation, olfaction), and braincase (housing the brain and sensory structures). © 2014 Wiley Periodicals, Inc.

  16. Dissection of Genetic Factors underlying Wheat Kernel Shape and Size in an Elite × Nonadapted Cross using a High Density SNP Linkage Map.

    PubMed

    Kumar, Ajay; Mantovani, E E; Seetan, R; Soltani, A; Echeverry-Solarte, M; Jain, S; Simsek, S; Doehlert, D; Alamri, M S; Elias, E M; Kianian, S F; Mergoum, M

    2016-03-01

    Wheat kernel shape and size has been under selection since early domestication. Kernel morphology is a major consideration in wheat breeding, as it impacts grain yield and quality. A population of 160 recombinant inbred lines (RIL), developed using an elite (ND 705) and a nonadapted genotype (PI 414566), was extensively phenotyped in replicated field trials and genotyped using Infinium iSelect 90K assay to gain insight into the genetic architecture of kernel shape and size. A high density genetic map consisting of 10,172 single nucleotide polymorphism (SNP) markers, with an average marker density of 0.39 cM/marker, identified a total of 29 genomic regions associated with six grain shape and size traits; ∼80% of these regions were associated with multiple traits. The analyses showed that kernel length (KL) and width (KW) are genetically independent, while a large number (∼59%) of the quantitative trait loci (QTL) for kernel shape traits were in common with genomic regions associated with kernel size traits. The most significant QTL was identified on chromosome 4B, and could be an ortholog of major rice grain size and shape gene or . Major and stable loci also were identified on the homeologous regions of Group 5 chromosomes, and in the regions of (6A) and (7A) genes. Both parental genotypes contributed equivalent positive QTL alleles, suggesting that the nonadapted germplasm has a great potential for enhancing the gene pool for grain shape and size. This study provides new knowledge on the genetic dissection of kernel morphology, with a much higher resolution, which may aid further improvement in wheat yield and quality using genomic tools. Copyright © 2016 Crop Science Society of America.

  17. Decomposition of Atmospheric Aerosol Phase Function by Particle Size and Morphology via Single Particle Scattering Measurements

    NASA Astrophysics Data System (ADS)

    Aptowicz, K. B.; Pan, Y.; Martin, S.; Fernandez, E.; Chang, R.; Pinnick, R. G.

    2013-12-01

    We report upon an experimental approach that provides insight into how particle size and shape affect the scattering phase function of atmospheric aerosol particles. Central to our approach is the design of an apparatus that measures the forward and backward scattering hemispheres (scattering patterns) of individual atmospheric aerosol particles in the coarse mode range. The size and shape of each particle is discerned from the corresponding scattering pattern. In particular, autocorrelation analysis is used to differentiate between spherical and non-spherical particles, the calculated asphericity factor is used to characterize the morphology of non-spherical particles, and the integrated irradiance is used for particle sizing. We found the fraction of spherical particles decays exponentially with particle size, decreasing from 11% for particles on the order of 1 micrometer to less than 1% for particles over 5 micrometer. The average phase functions of subpopulations of particles, grouped by size and morphology, are determined by averaging their corresponding scattering patterns. The phase functions of spherical and non-spherical atmospheric particles are shown to diverge with increasing size. In addition, the phase function of non-spherical particles is found to vary little as a function of the asphericity factor.

  18. The potential influence of morphology on the evolutionary divergence of an acoustic signal

    PubMed Central

    Pitchers, W. R.; Klingenberg, C.P.; Tregenza, Tom; Hunt, J.; Dworkin, I.

    2014-01-01

    The evolution of acoustic behaviour and that of the morphological traits mediating its production are often coupled. Lack of variation in the underlying morphology of signalling traits has the potential to constrain signal evolution. This relationship is particularly likely in field crickets, where males produce acoustic advertisement signals to attract females by stridulating with specialized structures on their forewings. In this study, we characterise the size and geometric shape of the forewings of males from six allopatric populations of the black field cricket (Teleogryllus commodus) known to have divergent advertisement calls. We sample from each of these populations using both wild-caught and common-garden reared cohorts, allowing us to test for multivariate relationships between wing morphology and call structure. We show that the allometry of shape has diverged across populations. However, there was a surprisingly small amount of covariation between wing shape and call structure within populations. Given the importance of male size for sexual selection in crickets, the divergence we observe among populations has the potential to influence the evolution of advertisement calls in this species. PMID:25223712

  19. The Selective Value of Bacterial Shape

    PubMed Central

    Young, Kevin D.

    2006-01-01

    Why do bacteria have shape? Is morphology valuable or just a trivial secondary characteristic? Why should bacteria have one shape instead of another? Three broad considerations suggest that bacterial shapes are not accidental but are biologically important: cells adopt uniform morphologies from among a wide variety of possibilities, some cells modify their shape as conditions demand, and morphology can be tracked through evolutionary lineages. All of these imply that shape is a selectable feature that aids survival. The aim of this review is to spell out the physical, environmental, and biological forces that favor different bacterial morphologies and which, therefore, contribute to natural selection. Specifically, cell shape is driven by eight general considerations: nutrient access, cell division and segregation, attachment to surfaces, passive dispersal, active motility, polar differentiation, the need to escape predators, and the advantages of cellular differentiation. Bacteria respond to these forces by performing a type of calculus, integrating over a number of environmental and behavioral factors to produce a size and shape that are optimal for the circumstances in which they live. Just as we are beginning to answer how bacteria create their shapes, it seems reasonable and essential that we expand our efforts to understand why they do so. PMID:16959965

  20. Whole organ, venation and epidermal cell morphological variations are correlated in the leaves of Arabidopsis mutants.

    PubMed

    Pérez-Pérez, José Manuel; Rubio-Díaz, Silvia; Dhondt, Stijn; Hernández-Romero, Diana; Sánchez-Soriano, Joaquín; Beemster, Gerrit T S; Ponce, María Rosa; Micol, José Luis

    2011-12-01

    Despite the large number of genes known to affect leaf shape or size, we still have a relatively poor understanding of how leaf morphology is established. For example, little is known about how cell division and cell expansion are controlled and coordinated within a growing leaf to eventually develop into a laminar organ of a definite size. To obtain a global perspective of the cellular basis of variations in leaf morphology at the organ, tissue and cell levels, we studied a collection of 111 non-allelic mutants with abnormally shaped and/or sized leaves, which broadly represent the mutational variations in Arabidopsis thaliana leaf morphology not associated with lethality. We used image-processing techniques on these mutants to quantify morphological parameters running the gamut from the palisade mesophyll and epidermal cells to the venation, whole leaf and rosette levels. We found positive correlations between epidermal cell size and leaf area, which is consistent with long-standing Avery's hypothesis that the epidermis drives leaf growth. In addition, venation parameters were positively correlated with leaf area, suggesting that leaf growth and vein patterning share some genetic controls. Positional cloning of the genes affected by the studied mutations will eventually establish functional links between genotypes, molecular functions, cellular parameters and leaf phenotypes. © 2011 Blackwell Publishing Ltd.

  1. Quantitative Outline-based Shape Analysis and Classification of Planetary Craterforms using Supervised Learning Models

    NASA Astrophysics Data System (ADS)

    Slezak, Thomas Joseph; Radebaugh, Jani; Christiansen, Eric

    2017-10-01

    The shapes of craterform morphology on planetary surfaces provides rich information about their origins and evolution. While morphologic information provides rich visual clues to geologic processes and properties, the ability to quantitatively communicate this information is less easily accomplished. This study examines the morphology of craterforms using the quantitative outline-based shape methods of geometric morphometrics, commonly used in biology and paleontology. We examine and compare landforms on planetary surfaces using shape, a property of morphology that is invariant to translation, rotation, and size. We quantify the shapes of paterae on Io, martian calderas, terrestrial basaltic shield calderas, terrestrial ash-flow calderas, and lunar impact craters using elliptic Fourier analysis (EFA) and the Zahn and Roskies (Z-R) shape function, or tangent angle approach to produce multivariate shape descriptors. These shape descriptors are subjected to multivariate statistical analysis including canonical variate analysis (CVA), a multiple-comparison variant of discriminant analysis, to investigate the link between craterform shape and classification. Paterae on Io are most similar in shape to terrestrial ash-flow calderas and the shapes of terrestrial basaltic shield volcanoes are most similar to martian calderas. The shapes of lunar impact craters, including simple, transitional, and complex morphology, are classified with a 100% rate of success in all models. Multiple CVA models effectively predict and classify different craterforms using shape-based identification and demonstrate significant potential for use in the analysis of planetary surfaces.

  2. Morphology-selective synthesis of polyhedral gold nanoparticles: what factors control the size and morphology of gold nanoparticles in a wet-chemical process.

    PubMed

    Lee, Jong-Hee; Kamada, Kai; Enomoto, Naoya; Hojo, Junichi

    2007-12-15

    Polyhedral gold nanoparticles below 100 nm in size were fabricated by continuously delivered HAuCl(4) and PVP starting solutions into l-ascorbic acid aqueous solution in the presence of gold seeds, and under addition of sodium hydroxide (NaOH). By continuously delivered PVP and HAuCl(4) starting solutions in the presence of gold seed, the size and shape of polyhedral gold were achieved in relatively good uniformity (particle size distribution=65-95 nm). Morphological evolution was also attempted using different growth rates of crystal facets with increasing reaction temperature, and selective adsorption of PVP.

  3. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Harilal, S. S.; Diwakar, P. K.; Polek, M. P.

    We investigated the role of spot size on plume morphology during ultrafast laser ablation of metal targets. Our results show that the spatial features of fs LA plumes are strongly dependent on the focal spot size. Two-dimensional self-emission images showed that the shape of the ultrafast laser ablation plumes changes from spherical to cylindrical with an increasing spot size from 100 to 600 μm. The changes in plume morphology and internal structures are related to ion emission dynamics from the plasma, where broader angular ion distribution and faster ions are noticed for the smallest spot size used. The present resultsmore » clearly show that the morphological changes in the plume with spot size are independent of laser pulse width.« less

  4. The Gavialis-Tomistoma debate: the contribution of skull ontogenetic allometry and growth trajectories to the study of crocodylian relationships.

    PubMed

    Piras, Paolo; Colangelo, Paolo; Adams, Dean C; Buscalioni, Angela; Cubo, Jorge; Kotsakis, Tassos; Meloro, Carlo; Raia, Pasquale

    2010-01-01

    The phylogenetic placement of Tomistoma and Gavialis crocodiles depends largely upon whether molecular or morphological data are utilized. Molecular analyses consider them as sister taxa, whereas morphological/paleontological analyses set Gavialis apart from Tomistoma and other crocodylian species. Here skull allometric trajectories of Tomistoma and Gavialis were contrasted with those of two longirostral crocodylian taxa, Crocodylus acutus and Mecistops cataphractus, to examine similarities in growth trajectories in light of this phylogenetic controversy. Entire skull shape and its two main modules, rostrum and postrostrum, were analyzed separately. We tested differences for both multivariate angles between trajectories and for shape differences at early and late stages of development. Based on a multivariate regression of shape data and size, Tomistoma seems to possess a peculiar rate of growth in comparison to the remaining taxa. However, its morphology at both juvenile and adult sizes is always closer to those of Brevirostres crocodylians, for the entire head shape, as well as the shape of the postrostrum and rostrum. By contrast, the allometric trajectory of Gavialis always begins and ends in a unique region of the multidimensional morphospace. These findings concur with a morphological hypothesis that places Gavialis separate from Brevirostres, and Tomistoma closer to other crocodylids, and provides an additional, and independent, data set to inform on this ongoing phylogenetic discussion. © 2010 Wiley Periodicals, Inc.

  5. Evolution of extreme body size disparity in monitor lizards (Varanus).

    PubMed

    Collar, David C; Schulte, James A; Losos, Jonathan B

    2011-09-01

    Many features of species' biology, including life history, physiology, morphology, and ecology are tightly linked to body size. Investigation into the causes of size divergence is therefore critical to understanding the factors shaping phenotypic diversity within clades. In this study, we examined size evolution in monitor lizards (Varanus), a clade that includes the largest extant lizard species, the Komodo dragon (V. komodoensis), as well as diminutive species that are nearly four orders of magnitude smaller in adult body mass. We demonstrate that the remarkable body size disparity of this clade is a consequence of different selective demands imposed by three major habitat use patterns-arboreality, terrestriality, and rock-dwelling. We reconstructed phylogenetic relationships and ancestral habitat use and applied model selection to determine that the best-fitting evolutionary models for species' adult size are those that infer oppositely directed adaptive evolution associated with terrestriality and rock-dwelling, with terrestrial lineages evolving extremely large size and rock-dwellers becoming very small. We also show that habitat use affects the evolution of several ecologically important morphological traits independently of body size divergence. These results suggest that habitat use exerts a strong, multidimensional influence on the evolution of morphological size and shape disparity in monitor lizards. © 2011 The Author(s).

  6. Characterization of wood dust from furniture by scanning electron microscopy and energy-dispersive x-ray analysis.

    PubMed

    Gómez Yepes, Milena Elizabeth; Cremades, Lázaro V

    2011-01-01

    Study characterized and analyzed form factor, elementary composition and particle size of wood dust, in order to understand its harmful health effects on carpenters in Quindío (Colombia). Once particle characteristics (size distributions, aerodynamic equivalent diameter (D(α)), elemental composition and shape factors) were analyzed, particles were then characterized via scanning electron microscopy (SEM) in conjunction with energy dispersive X-ray analysis (EDXRA). SEM analysis of particulate matter showed: 1) cone-shaped particle ranged from 2.09 to 48.79 µm D(α); 2) rectangular prism-shaped particle from 2.47 to 72.9 µm D(α); 3) cylindrically-shaped particle from 2.5 to 48.79 µm D(α); and 4) spherically-shaped particle from 2.61 to 51.93 µm D(α). EDXRA reveals presence of chemical elements from paints and varnishes such as Ca, K, Na and Cr. SEM/EDXRA contributes in a significant manner to the morphological characterization of wood dust. It is obvious that the type of particles sampled is a complex function of shapes and sizes of particles. Thus, it is important to investigate the influence of particles characteristics, morphology, shapes and D(α) that may affect the health of carpenters in Quindío.

  7. A non-invasive geometric morphometrics method for exploring variation in dorsal head shape in urodeles: sexual dimorphism and geographic variation in Salamandra salamandra.

    PubMed

    Alarcón-Ríos, Lucía; Velo-Antón, Guillermo; Kaliontzopoulou, Antigoni

    2017-04-01

    The study of morphological variation among and within taxa can shed light on the evolution of phenotypic diversification. In the case of urodeles, the dorso-ventral view of the head captures most of the ontogenetic and evolutionary variation of the entire head, which is a structure with a high potential for being a target of selection due to its relevance in ecological and social functions. Here, we describe a non-invasive procedure of geometric morphometrics for exploring morphological variation in the external dorso-ventral view of urodeles' head. To explore the accuracy of the method and its potential for describing morphological patterns we applied it to two populations of Salamandra salamandra gallaica from NW Iberia. Using landmark-based geometric morphometrics, we detected differences in head shape between populations and sexes, and an allometric relationship between shape and size. We also determined that not all differences in head shape are due to size variation, suggesting intrinsic shape differences across sexes and populations. These morphological patterns had not been previously explored in S. salamandra, despite the high levels of intraspecific diversity within this species. The methodological procedure presented here allows to detect shape variation at a very fine scale, and solves the drawbacks of using cranial samples, thus increasing the possibilities of using collection specimens and alive animals for exploring dorsal head shape variation and its evolutionary and ecological implications in urodeles. J. Morphol. 278:475-485, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  8. The shapes of bird beaks are highly controlled by nondietary factors

    PubMed Central

    Bright, Jen A.; Marugán-Lobón, Jesús; Cobb, Samuel N.

    2016-01-01

    Bird beaks are textbook examples of ecological adaptation to diet, but their shapes are also controlled by genetic and developmental histories. To test the effects of these factors on the avian craniofacial skeleton, we conducted morphometric analyses on raptors, a polyphyletic group at the base of the landbird radiation. Despite common perception, we find that the beak is not an independently targeted module for selection. Instead, the beak and skull are highly integrated structures strongly regulated by size, with axes of shape change linked to the actions of recently identified regulatory genes. Together, size and integration account for almost 80% of the shape variation seen between different species to the exclusion of morphological dietary adaptation. Instead, birds of prey use size as a mechanism to modify their feeding ecology. The extent to which shape variation is confined to a few major axes may provide an advantage in that it facilitates rapid morphological evolution via changes in body size, but may also make raptors especially vulnerable when selection pressures act against these axes. The phylogenetic position of raptors suggests that this constraint is prevalent in all landbirds and that breaking the developmental correspondence between beak and braincase may be the key novelty in classic passerine adaptive radiations. PMID:27125856

  9. The shapes of bird beaks are highly controlled by nondietary factors.

    PubMed

    Bright, Jen A; Marugán-Lobón, Jesús; Cobb, Samuel N; Rayfield, Emily J

    2016-05-10

    Bird beaks are textbook examples of ecological adaptation to diet, but their shapes are also controlled by genetic and developmental histories. To test the effects of these factors on the avian craniofacial skeleton, we conducted morphometric analyses on raptors, a polyphyletic group at the base of the landbird radiation. Despite common perception, we find that the beak is not an independently targeted module for selection. Instead, the beak and skull are highly integrated structures strongly regulated by size, with axes of shape change linked to the actions of recently identified regulatory genes. Together, size and integration account for almost 80% of the shape variation seen between different species to the exclusion of morphological dietary adaptation. Instead, birds of prey use size as a mechanism to modify their feeding ecology. The extent to which shape variation is confined to a few major axes may provide an advantage in that it facilitates rapid morphological evolution via changes in body size, but may also make raptors especially vulnerable when selection pressures act against these axes. The phylogenetic position of raptors suggests that this constraint is prevalent in all landbirds and that breaking the developmental correspondence between beak and braincase may be the key novelty in classic passerine adaptive radiations.

  10. Sexual dimorphism in America: geometric morphometric analysis of the craniofacial region.

    PubMed

    Kimmerle, Erin H; Ross, Ann; Slice, Dennis

    2008-01-01

    One of the four pillars of the anthropological protocol is the estimation of sex. The protocol generally consists of linear metric analysis or visually assessing individual skeletal traits on the skull and pelvis based on an ordinal scale of 1-5, ranging from very masculine to very feminine. The morphologic traits are then some how averaged by the investigator to estimate sex. Some skulls may be misclassified because of apparent morphologic features that appear more or less robust due to size differences among individuals. The question of misclassification may be further exemplified in light of comparisons across populations that may differ not only in cranial robusticity but also in stature and general physique. The purpose of this study is to further examine the effect of size and sex on craniofacial shape among American populations to better understand the allometric foundation of skeletal traits currently used for sex estimation. Three-dimensional coordinates of 16 standard craniofacial landmarks were collected using a Microscribe-3DX digitizer. Data were collected for 118 American White and Black males and females from the W.M. Bass Donated Collection and the Forensic Data Bank. The MANCOVA procedure tested shape differences as a function of sex and size. Sex had a significant influence on shape for both American Whites (F = 2.90; d.f. = 19, 39; p > F = 0.0024) and Blacks (F = 2.81; d.f. = 19, 37; p > F = 0.0035), whereas size did not have a significant influence on shape in either Whites (F = 1.69; d.f. = 19, 39; p > F = 0.08) or Blacks (F = 1.09; d.f. = 19, 37; p > F = 0.40). Therefore, for each sex, individuals of various sizes were statistically the same shape. In other words, while significant differences were present between the size of males and females (males on average were larger), there was no size effect beyond that accounted for by sex differences in size. Moreover, the consistency between American groups is interesting as it suggests that population differences in sexual dimorphism may result more from human variation in size than allometric variation in craniofacial morphology.

  11. Dissection of genetic factors underlying wheat kernel shape and size in an elite x nonadapted cross using a high density SNP linkage map

    USDA-ARS?s Scientific Manuscript database

    Wheat kernel shape and size has been under selection since early domestication. Kernel morphology is a major consideration in wheat breeding, as it impacts grain yield and quality. A population of 160 recombinant inbred lines (RIL), developed using an elite (ND 705) and a nonadapted genotype (PI 414...

  12. Three-dimensional geometric morphometric analysis of talar morphology in extant gorilla taxa from highland and lowland habitats.

    PubMed

    Knigge, Ryan P; Tocheri, Matthew W; Orr, Caley M; Mcnulty, Kieran P

    2015-01-01

    Western gorillas (Gorilla gorilla) are known to climb significantly more often than eastern gorillas (Gorilla beringei), a behavioral distinction attributable to major differences in their respective habitats (i.e., highland vs. lowland). Genetic evidence suggests that the lineages leading to these taxa began diverging from one another between approximately 1 and 3 million years ago. Thus, gorillas offer a special opportunity to examine the degree to which morphology of recently diverged taxa may be "fine-tuned" to differing ecological requirements. Using three-dimensional (3D) geometric morphometrics, we compared talar morphology in a sample of 87 specimens including western (lowland), mountain (highland), and grauer gorillas (lowland and highland populations). Talar shape was captured with a series of landmarks and semilandmarks superimposed by generalized Procrustes analysis. A between-group principal components analysis of overall talar shape separates gorillas by ecological habitat and by taxon. An analysis of only the trochlea and lateral malleolar facet identifies subtle variations in trochlear shape between western lowland and lowland grauer gorillas, potentially indicative of convergent evolution of arboreal adaptations in the talus. Lastly, talar shape scales differently with centroid size for highland and lowland gorillas, suggesting that ankle morphology may track body-size mediated variation in arboreal behaviors differently depending on ecological setting. Several of the observed shape differences are linked biomechanically to the facilitation of climbing in lowland gorillas and to stability and load-bearing on terrestrial substrates in the highland taxa, providing an important comparative model for studying morphological variation in groups known only from fossils (e.g., early hominins). © 2014 Wiley Periodicals, Inc.

  13. A systematic account of the genus Plagiostoma (Gnomoniaceae, Diaporthales) based on morphology, host-associations, and a four gene phylogeny

    USDA-ARS?s Scientific Manuscript database

    The genus Plagiostoma inhabits leaves and branches of a range of woody and herbaceous plant families in the temperate northern hemisphere. Based on analyses of morphological, cultural, and molecular data, Plagiostoma is reviewed and monographed. The morphological data include shape and size of perit...

  14. Hypersalinity drives physiological and morphological changes in Limia perugiae (Poeciliidae)

    PubMed Central

    Tello, Oscar; Krieger, Jonathan; Marmolejo, Arlen; Weaver, Kathleen F.; Garcia, Jerome V.; Cruz, Alexander

    2016-01-01

    ABSTRACT A fundamental question in biology is how an organism's morphology and physiology are shaped by its environment. Here, we evaluate the effects of a hypersaline environment on the morphology and physiology of a population of livebearing fish in the genus Limia (Poeciliidae). We sampled from two populations of Limia perugiae (one freshwater and one hypersaline) in the southwest Dominican Republic. We evaluated relative abundance of osmoregulatory proteins using western blot analyses and used a geometric morphometric approach to evaluate fine-scale changes to size and shape. Our data show that gill tissue isolated from hypersaline fish contained approximately two and a half times higher expression of Na+/K+ ATPase proteins. We also show evidence for mitochondrial changes within the gills, with eight times more complex I and four times higher expression of ATP synthase within the gill tissue from the hypersaline population. The energetic consequences to Limia living in saline and hypersaline environments may be a driver for phenotypic diversity, reducing the overall body size and changing the relative size and shape of the head, as well as impeding the growth of secondary sex features among the males. PMID:27402966

  15. Semicircular canals in Anolis lizards: ecomorphological convergence and ecomorph affinities of fossil species.

    PubMed

    Dickson, Blake V; Sherratt, Emma; Losos, Jonathan B; Pierce, Stephanie E

    2017-10-01

    Anoli s lizards are a model system for the study of adaptive radiation and convergent evolution. Greater Antillean anoles have repeatedly evolved six similar forms or ecomorphs: crown-giant, grass-bush, twig, trunk, trunk-crown and trunk-ground. Members of each ecomorph category possess a specific set of morphological, ecological and behavioural characteristics which have been acquired convergently. Here we test whether the semicircular canal system-the organ of balance during movement-is also convergent among ecomorphs, reflecting the shared sensory requirements of their ecological niches. As semicircular canal shape has been shown to reflect different locomotor strategies, we hypothesized that each Anolis ecomorph would have a unique canal morphology. Using three-dimensional semilandmarks and geometric morphometrics, semicircular canal shape was characterized in 41 Anolis species from the Greater Antilles and the relationship between canal shape and ecomorph grouping, phylogenetic history, size, head dimensions, and perch characteristics was assessed. Further, canal morphology of modern species was used to predict the ecomorph affinity of five fossil anoles from the Miocene of the Dominican Republic. Of the covariates tested, our study recovered ecomorph as the single-most important covariate of canal morphology in modern taxa; although phylogenetic history, size, and head dimensions also showed a small, yet significant correlation with shape. Surprisingly, perch characteristics were not found to be significant covariates of canal shape, even though they are important habitat variables. Using posterior probabilities, we found that the fossil anoles have different semicircular canals shapes to modern ecomorph groupings implying extinct anoles may have been interacting with their Miocene environment in different ways to modern Anolis species.

  16. Semicircular canals in Anolis lizards: ecomorphological convergence and ecomorph affinities of fossil species

    NASA Astrophysics Data System (ADS)

    Dickson, Blake V.; Sherratt, Emma; Losos, Jonathan B.; Pierce, Stephanie E.

    2017-10-01

    Anolis lizards are a model system for the study of adaptive radiation and convergent evolution. Greater Antillean anoles have repeatedly evolved six similar forms or ecomorphs: crown-giant, grass-bush, twig, trunk, trunk-crown and trunk-ground. Members of each ecomorph category possess a specific set of morphological, ecological and behavioural characteristics which have been acquired convergently. Here we test whether the semicircular canal system-the organ of balance during movement-is also convergent among ecomorphs, reflecting the shared sensory requirements of their ecological niches. As semicircular canal shape has been shown to reflect different locomotor strategies, we hypothesized that each Anolis ecomorph would have a unique canal morphology. Using three-dimensional semilandmarks and geometric morphometrics, semicircular canal shape was characterized in 41 Anolis species from the Greater Antilles and the relationship between canal shape and ecomorph grouping, phylogenetic history, size, head dimensions, and perch characteristics was assessed. Further, canal morphology of modern species was used to predict the ecomorph affinity of five fossil anoles from the Miocene of the Dominican Republic. Of the covariates tested, our study recovered ecomorph as the single-most important covariate of canal morphology in modern taxa; although phylogenetic history, size, and head dimensions also showed a small, yet significant correlation with shape. Surprisingly, perch characteristics were not found to be significant covariates of canal shape, even though they are important habitat variables. Using posterior probabilities, we found that the fossil anoles have different semicircular canals shapes to modern ecomorph groupings implying extinct anoles may have been interacting with their Miocene environment in different ways to modern Anolis species.

  17. Trophic specialization drives morphological evolution in sea snakes.

    PubMed

    Sherratt, Emma; Rasmussen, Arne R; Sanders, Kate L

    2018-03-01

    Viviparous sea snakes are the most rapidly speciating reptiles known, yet the ecological factors underlying this radiation are poorly understood. Here, we reconstructed dated trees for 75% of sea snake species and quantified body shape (forebody relative to hindbody girth), maximum body length and trophic diversity to examine how dietary specialization has influenced morphological diversification in this rapid radiation. We show that sea snake body shape and size are strongly correlated with the proportion of burrowing prey in the diet. Specialist predators of burrowing eels have convergently evolved a 'microcephalic' morphotype with dramatically reduced forebody relative to hindbody girth and intermediate body length. By comparison, snakes that predominantly feed on burrowing gobies are generally short-bodied and small-headed, but there is no evidence of convergent evolution. The eel specialists also exhibit faster rates of size and shape evolution compared to all other sea snakes, including those that feed on gobies. Our results suggest that trophic specialization to particular burrowing prey (eels) has invoked strong selective pressures that manifest as predictable and rapid morphological changes. Further studies are needed to examine the genetic and developmental mechanisms underlying these dramatic morphological changes and assess their role in sea snake speciation.

  18. The evolution of cranial base and face in Cercopithecoidea and Hominoidea: Modularity and morphological integration.

    PubMed

    Profico, Antonio; Piras, Paolo; Buzi, Costantino; Di Vincenzo, Fabio; Lattarini, Flavio; Melchionna, Marina; Veneziano, Alessio; Raia, Pasquale; Manzi, Giorgio

    2017-12-01

    The evolutionary relationship between the base and face of the cranium is a major topic of interest in primatology. Such areas of the skull possibly respond to different selective pressures. Yet, they are often said to be tightly integrated. In this paper, we analyzed shape variability in the cranial base and the facial complex in Cercopithecoidea and Hominoidea. We used a landmark-based approach to single out the effects of size (evolutionary allometry), morphological integration, modularity, and phylogeny (under Brownian motion) on skull shape variability. Our results demonstrate that the cranial base and the facial complex exhibit different responses to different factors, which produces a little degree of morphological integration between them. Facial shape variation appears primarily influenced by body size and sexual dimorphism, whereas the cranial base is mostly influenced by functional factors. The different adaptations affecting the two modules suggest they are best studied as separate and independent units, and that-at least when dealing with Catarrhines-caution must be posed with the notion of strong cranial integration that is commonly invoked for the evolution of their skull shape. © 2017 Wiley Periodicals, Inc.

  19. Two new species of free-living nematodes genera Microlaimus de Man, 1880 and Aponema Jensen, 1978 (Nematoda: Microlaimidae) from the Black Sea.

    PubMed

    Revkova, Tatiana N

    2017-11-07

    Two new species of the family Microlaimidae Micoletzky, 1922 are described and illustrated from the Black Sea. Aponema pontica sp. n. is morphologically closest to A. torosum in the shape of the body and spicules, size of amphids, but differs in having small and triangular cardia, absence of constriction in head region, shape of gubernaculum apophyses, rounded and weakly sclerotised lumen of pharyngeal bulb and longer spicules. Microlaimus paraglobiceps sp. n. morphologically resembles M. globiceps de Man, 1880 in the shape of the body, structure of the male sexual organs and presence of precloacal pore, but the main difference is a shorter body, cuticle finely annulated all over the body and absence of sexual dimorphism in the size of amphideal fovea.

  20. Evolution of eye morphology and rhodopsin expression in the Drosophila melanogaster species subgroup.

    PubMed

    Posnien, Nico; Hopfen, Corinna; Hilbrant, Maarten; Ramos-Womack, Margarita; Murat, Sophie; Schönauer, Anna; Herbert, Samantha L; Nunes, Maria D S; Arif, Saad; Breuker, Casper J; Schlötterer, Christian; Mitteroecker, Philipp; McGregor, Alistair P

    2012-01-01

    A striking diversity of compound eye size and shape has evolved among insects. The number of ommatidia and their size are major determinants of the visual sensitivity and acuity of the compound eye. Each ommatidium is composed of eight photoreceptor cells that facilitate the discrimination of different colours via the expression of various light sensitive Rhodopsin proteins. It follows that variation in eye size, shape, and opsin composition is likely to directly influence vision. We analyzed variation in these three traits in D. melanogaster, D. simulans and D. mauritiana. We show that D. mauritiana generally has larger eyes than its sibling species, which is due to a combination of larger ommatidia and more ommatidia. In addition, intra- and inter-specific differences in eye size among D. simulans and D. melanogaster strains are mainly caused by variation in ommatidia number. By applying a geometric morphometrics approach to assess whether the formation of larger eyes influences other parts of the head capsule, we found that an increase in eye size is associated with a reduction in the adjacent face cuticle. Our shape analysis also demonstrates that D. mauritiana eyes are specifically enlarged in the dorsal region. Intriguingly, this dorsal enlargement is associated with enhanced expression of rhodopsin 3 in D. mauritiana. In summary, our data suggests that the morphology and functional properties of the compound eyes vary considerably within and among these closely related Drosophila species and may be part of coordinated morphological changes affecting the head capsule.

  1. Sexual selection and the evolution of genital shape and complexity in water striders.

    PubMed

    Rowe, Locke; Arnqvist, Göran

    2012-01-01

    Animal genitalia show two striking but incompletely understood evolutionary trends: a great evolutionary divergence in the shape of genitalic structures, and characteristic structural complexity. Both features are thought to result from sexual selection, but explicit comparative tests are hampered by the fact that it is difficult to quantify both morphological complexity and divergence in shape. We undertake a comparative study of multiple nongenitalic and male genital traits in a clade of 15 water strider species to quantify complexity and shape divergence. We show that genital structures are more complex and their shape more divergent among species than nongenital traits. Further, intromittent genital traits are more complex and have evolved more divergently than nonintromittent genital traits. More importantly, shape and complexity of nonintromittent genital traits show correlated evolution with indices of premating sexual selection and intromittent genital traits with postmating sexual selection, suggesting that the evolution of different components of genital morphology are shaped independently by distinct forms of sexual selection. Our quantitative results provide direct comparative support for the hypothesis that sexual selection is associated with morphological complexity in genitalic traits and highlight the importance of quantifying morphological shape and complexity, rather than size in studies of genital evolution. © 2011 The Author(s). Evolution © 2011 The Society for the Study of Evolution.

  2. Cuban Calisto (Lepidoptera, Nymphalidae, Satyrinae), a review based on morphological and DNA data

    PubMed Central

    Aguila, Rayner Núñez; Plasencia, Edelquis Oliva; Maravi, Pavel F. Matos; Wahlberg, Niklas

    2012-01-01

    Abstract The Cuban species of Calisto are reviewed based on the morphology of adult and immature stages, as well as DNA sequences of six genes (COI, EF1α, wingless, GAPDH, RpS5, CAD). A new species, Calisto occulta sp. n., is described from the northeastern Cuban mountains. Calisto smintheus Bates, 1935 and Calisto bruneri, Michener 1949 are revised and revalidated. A new status, the species level, is proposed for Calisto brochei, Torre 1973, Calisto muripetens, Bates 1939 and Calisto bradleyi, Munroe 1950. The immature stages of Calisto smintheus, Calisto brochei,and Calisto occulta are described for the first time, and those of Calisto herophile, Hübner 1823 are redescribed. Useful morphological characters for adults are the shape and conspicuousness of androconial patch, the number and relative size of white dots on underside of hindwing, the shape of aedeagus, the shape of digitiform projection of genitalia valve, the shape and relative size of tegumen and uncus, the relative size of female genitalia, the height of sterigmal ring dorsal crown of the latter, and the relative size of corpus bursae and ductus bursae. For the immature stages, the most important characters are the color pattern of head capsule, the number and width of longitudinal lines of body, in the larvae; and the color pattern and the absence or presence of dorsal ridges on the abdomen of pupae. The phylogenetic relationships between the Cuban Calisto species are quite robust and well-supported; however, conflict between mitochondrial and nuclear datasets was detected in Calisto brochei, Calisto muripetens and to a lesser degree in Calisto bradleyi. PMID:22328857

  3. Platinum and palladium nano-structured catalysts for polymer electrolyte fuel cells and direct methanol fuel cells.

    PubMed

    Long, Nguyen Viet; Thi, Cao Minh; Yong, Yang; Nogami, Masayuki; Ohtaki, Michitaka

    2013-07-01

    In this review, we present the synthesis and characterization of Pt, Pd, Pt based bimetallic and multi-metallic nanoparticles with mixture, alloy and core-shell structure for nano-catalysis, energy conversion, and fuel cells. Here, Pt and Pd nanoparticles with modified nanostructures can be controllably synthesized via chemistry and physics for their uses as electro-catalysts. The cheap base metal catalysts can be studied in the relationship of crystal structure, size, morphology, shape, and composition for new catalysts with low cost. Thus, Pt based alloy and core-shell catalysts can be prepared with the thin Pt and Pt-Pd shell, which are proposed in low and high temperature proton exchange membrane fuel cells (PEMFCs), and direct methanol fuel cells (DMFCs). We also present the survey of the preparation of Pt and Pd based catalysts for the better catalytic activity, high durability, and stability. The structural transformations, quantum-size effects, and characterization of Pt and Pd based catalysts in the size ranges of 30 nm (1-30 nm) are presented in electro-catalysis. In the size range of 10 nm (1-10 nm), the pure Pt catalyst shows very large surface area for electro-catalysis. To achieve homogeneous size distribution, the shaped synthesis of the polyhedral Pt nanoparticles is presented. The new concept of shaping specific shapes and morphologies in the entire nano-scale from nano to micro, such as polyhedral, cube, octahedra, tetrahedra, bar, rod, and others of the nanoparticles is proposed, especially for noble and cheap metals. The uniform Pt based nanosystems of surface structure, internal structure, shape, and morphology in the nanosized ranges are very crucial to next fuel cells. Finally, the modifications of Pt and Pd based catalysts of alloy, core-shell, and mixture structures lead to find high catalytic activity, durability, and stability for nano-catalysis, energy conversion, fuel cells, especially the next large-scale commercialization of next PEMFCs, and DMFCs.

  4. Small queens and big-headed workers in a monomorphic ponerine ant

    NASA Astrophysics Data System (ADS)

    Kikuchi, Tomonori; Miyazaki, Satoshi; Ohnishi, Hitoshi; Takahashi, Junichi; Nakajima, Yumiko; Tsuji, Kazuki

    2008-10-01

    Evolution of caste is a central issue in the biology of social insects. Comparative studies on their morphology so far suggest the following three patterns: (1) a positive correlation between queen worker size dimorphism and the divergence in reproductive ability between castes, (2) a negative correlation among workers between morphological diversity and reproductive ability, and (3) a positive correlation between queen worker body shape difference and the diversity in worker morphology. We conducted morphological comparisons between castes in Pachycondyla luteipes, workers of which are monomorphic and lack their reproductive ability. Although the size distribution broadly overlapped, mean head width, head length, and scape length were significantly different between queens and workers. Conversely, in eye length, petiole width, and Weber’s length, the size differences were reversed. The allometries (head length/head width, scape length/head width, and Weber’s length/head width) were also significantly different between queens and workers. Morphological examinations showed that the body shape was different between queens and workers, and the head part of workers was disproportionately larger than that of queens. This pattern of queen worker dimorphism is novel in ants with monomorphic workers and a clear exception to the last pattern. This study suggests that it is possible that the loss of individual-level selection, the lack of reproductive ability, influences morphological modification in ants.

  5. Strengthening Precipitate Morphologies Fully Quantified in Advanced Disk Superalloys

    NASA Technical Reports Server (NTRS)

    Gabb, Timothy P.

    1998-01-01

    Advanced aviation gas turbine engines will require disk superalloys that can operate at higher temperatures and stresses than current conditions. Such applications will be limited by the tensile, creep, and fatigue mechanical properties of these alloys. These mechanical properties vary with the size, shape, and quantity of the gamma precipitates that strengthen disk superalloys. It is therefore important to quantify these precipitate parameters and relate them to mechanical properties to improve disk superalloys. Favorable precipitate morphologies and practical processing approaches to achieve them can then be determined. A methodology has been developed at the NASA Lewis Research Center to allow the comprehensive quantification of the size, shape, and quantity of all types of gamma precipitates.

  6. Food Web Structure Shapes the Morphology of Teleost Fish Brains.

    PubMed

    Edmunds, Nicholas B; McCann, Kevin S; Laberge, Frédéric

    2016-01-01

    Previous work showed that teleost fish brain size correlates with the flexible exploitation of habitats and predation abilities in an aquatic food web. Since it is unclear how regional brain changes contribute to these relationships, we quantitatively examined the effects of common food web attributes on the size of five brain regions in teleost fish at both within-species (plasticity or natural variation) and between-species (evolution) scales. Our results indicate that brain morphology is influenced by habitat use and trophic position, but not by the degree of littoral-pelagic habitat coupling, despite the fact that the total brain size was previously shown to increase with habitat coupling in Lake Huron. Intriguingly, the results revealed two potential evolutionary trade-offs: (i) relative olfactory bulb size increased, while relative optic tectum size decreased, across a trophic position gradient, and (ii) the telencephalon was relatively larger in fish using more littoral-based carbon, while the cerebellum was relatively larger in fish using more pelagic-based carbon. Additionally, evidence for a within-species effect on the telencephalon was found, where it increased in size with trophic position. Collectively, these results suggest that food web structure has fundamentally contributed to the shaping of teleost brain morphology. © 2016 S. Karger AG, Basel.

  7. Wing morphometrics of Aedes (Ochlerotatus) albifasciatus (Macquart, 1838) (Diptera: Culicidae) from different climatic regions of Argentina.

    PubMed

    Garzón, Maximiliano J; Schweigmann, Nicolás

    2018-05-16

    Gene flow restrictions between populations of Aedes albifasciatus, the vector of Western equine encephalitis and Dirophilaria immitis, have been described in the central region of Argentina. Genetic and eco-physiological variations usually result in local forms reflecting the climatic regions. Mosquito wings and their different parts have ecological functions in flight and communication. Therefore, wing shape could be considered an aspect of sexual dimorphism, and its eco-physiological responses can be expressed as morphological changes induced by the environment. To compare the geographical and sexual variations with respect to wing shape and size in two Ae. albifasciatus populations from contrasting climates of Argentina (temperate: Buenos Aires, and the arid steppe of Patagonia: Sarmiento), the wings of adults reared in thermal trays at different constant temperatures (10-29 °C) were analyzed. The wing size of Ae. albifasciatus showed inverse linear relationships with the rearing thermal condition and higher slope for Buenos Aires. In the cool range (10-17 °C), geographical size variations responded to the converse Bergmann's rule, where Buenos Aires individuals were larger than those from Sarmiento. Sexual shape dimorphism occurred in both populations while geographical variation in shape was observed in both sexes. Buenos Aires individuals showed greater response sensitivity with respect to the size-temperature relation than those from Sarmiento. The converse Bergmann's rule in size variation could be due to a higher development rate in Sarmiento to produce more cohorts in the limited favorable season. The shape could be more relevant with respect to the size in the study of population structures due to the size being more liable to vary due to changes in the environment. The geographical variations with respect to morphology could be favored by the isolation between populations and adaptations to the environmental conditions. Our results demonstrate that the shape and size of wing provide useful phenotypic information for studies related to sexual and environmental adaptations.

  8. Multi-Scale Porous Ultra High Temperature Ceramics

    DTIC Science & Technology

    2015-01-08

    different techniques: replica, particle stabilized foams, ice templating (freeze casting) and partial sintering. The pore morphology (closed-bubble...the porosity, pore size, shape and morphology . X-Ray Tomography was used to study their 3D microstructure. The 3D microstructures captured with...four different techniques: replica, particle stabilized foams, ice templating (freeze casting) and partial sintering. The pore morphology (closed-bubble

  9. The influence of pressure and gas flow on size and morphology of titanium oxide nanoparticles synthesized by hollow cathode sputtering

    NASA Astrophysics Data System (ADS)

    Gunnarsson, Rickard; Pilch, Iris; Boyd, Robert D.; Brenning, Nils; Helmersson, Ulf

    2016-07-01

    Titanium oxide nanoparticles have been synthesized via sputtering of a hollow cathode in an argon atmosphere. The influence of pressure and gas flow has been studied. Changing the pressure affects the nanoparticle size, increasing approximately proportional to the pressure squared. The influence of gas flow is dependent on the pressure. In the low pressure regime (107 ≤ p ≤ 143 Pa), the nanoparticle size decreases with increasing gas flow; however, at high pressure (p = 215 Pa), the trend is reversed. For low pressures and high gas flows, it was necessary to add oxygen for the particles to nucleate. There is also a morphological transition of the nanoparticle shape that is dependent on the pressure. Shapes such as faceted, cubic, and cauliflower can be obtained.

  10. Heating efficiency dependency on size and morphology of magnetite nanoparticles

    NASA Astrophysics Data System (ADS)

    Parekh, Kinnari; Parmar, Harshida; Sharma, Vinay; Ramanujan, R. V.

    2018-04-01

    Different size magnetite nanoparticles ranging from superparamagnetic (9 nm) to single domain (27 nm) and multi domain (53 nm) were synthesized using chemical route. Morphology of these particles as seen from TEM images indicates shape change from spherical to cubic with the growth of particles. The saturation magnetization (σs) and Specific Loss Power (SLP) showed maximum for single domain size, 72 emu/g and 102 W/g, respectively then those of multi domain size particles. These samples show higher SLP at relatively low concentration, low frequency and low amplitude compared to samples prepared by other routes.

  11. Geometric morphometrics reveals shifts in flower shape symmetry and size following gene knockdown of CYCLOIDEA and ANTHOCYANIDIN SYNTHASE.

    PubMed

    Berger, Brent A; Ricigliano, Vincent A; Savriama, Yoland; Lim, Aedric; Thompson, Veronica; Howarth, Dianella G

    2017-11-17

    While floral symmetry has traditionally been assessed qualitatively, recent advances in geometric morphometrics have opened up new avenues to specifically quantify flower shape and size using robust multivariate statistical methods. In this study, we examine, for the first time, the ability of geometric morphometrics to detect morphological differences in floral dorsoventral asymmetry following virus-induced gene silencing (VIGS). Using Fedia graciliflora Fisch. & Meyer (Valerianaceae) as a model, corolla shape of untreated flowers was compared using canonical variate analysis to knockdown phenotypes of CYCLOIDEA2A (FgCYC2A), ANTHOCYANIDIN SYNTHASE (FgANS), and empty vector controls. Untreated flowers and all VIGS treatments were morphologically distinct from each other, suggesting that VIGS may cause subtle shifts in floral shape. Knockdowns of FgCYC2A were the most dramatic, affecting the position of dorsal petals in relation to lateral petals, thereby resulting in more actinomorphic-like flowers. Additionally, FgANS knockdowns developed larger flowers with wider corolla tube openings. These results provide a method to quantify the role that specific genes play in the developmental pathway affecting the dorsoventral axis of symmetry in zygomorphic flowers. Additionally, they suggest that ANS may have an unintended effect on floral size and shape.

  12. Morphological divergence between three Arctic charr morphs - the significance of the deep-water environment.

    PubMed

    Skoglund, Sigrid; Siwertsson, Anna; Amundsen, Per-Arne; Knudsen, Rune

    2015-08-01

    Morphological divergence was evident among three sympatric morphs of Arctic charr (Salvelinus alpinus (L.)) that are ecologically diverged along the shallow-, deep-water resource axis in a subarctic postglacial lake (Norway). The two deep-water (profundal) spawning morphs, a benthivore (PB-morph) and a piscivore (PP-morph), have evolved under identical abiotic conditions with constant low light and temperature levels in their deep-water habitat, and were morphologically most similar. However, they differed in important head traits (e.g., eye and mouth size) related to their different diet specializations. The small-sized PB-morph had a paedomorphic appearance with a blunt head shape, large eyes, and a deep body shape adapted to their profundal lifestyle feeding on submerged benthos from soft, deep-water sediments. The PP-morph had a robust head, large mouth with numerous teeth, and an elongated body shape strongly related to their piscivorous behavior. The littoral spawning omnivore morph (LO-morph) predominantly utilizes the shallow benthic-pelagic habitat and food resources. Compared to the deep-water morphs, the LO-morph had smaller head relative to body size. The LO-morph exhibited traits typical for both shallow-water benthic feeding (e.g., large body depths and small eyes) and planktivorous feeding in the pelagic habitat (e.g., streamlined body shape and small mouth). The development of morphological differences within the same deep-water habitat for the PB- and PP-morphs highlights the potential of biotic factors and ecological interactions to promote further divergence in the evolution of polymorphism in a tentative incipient speciation process. The diversity of deep-water charr in this study represents a novelty in the Arctic charr polymorphism as a truly deep-water piscivore morph has to our knowledge not been described elsewhere.

  13. A Facile Methodology for Engineering the Morphology of CsPbX3 Perovskite Nanocrystals under Ambient Condition

    NASA Astrophysics Data System (ADS)

    Seth, Sudipta; Samanta, Anunay

    2016-11-01

    A facile and highly reproducible room temperature, open atmosphere synthesis of cesium lead halide perovskite nanocrystals of six different morphologies is reported just by varying the solvent, ligand and reaction time. Sequential evolution of the quantum dots, nanoplates and nanobars in one medium and nanocubes, nanorods and nanowires in another medium is demonstrated. These perovskite nanoparticles are shown to be of excellent crystalline quality with high fluorescence quantum yield. A mechanism of the formation of nanoparticles of different shapes and sizes is proposed. Considering the key role of morphology in nanotechnology, this simple method of fabrication of a wide range of high quality nanocrystals of different shapes and sizes of all-inorganic lead halide perovskites, whose potential is already demonstrated in light emitting and photovoltaic applications, is likely to help widening the scope and utility of these materials in optoelectronic devices.

  14. Identification of major and minor QTL for ecologically important morphological traits in three-spined sticklebacks (Gasterosteus aculeatus).

    PubMed

    Liu, Jun; Shikano, Takahito; Leinonen, Tuomas; Cano, José Manuel; Li, Meng-Hua; Merilä, Juha

    2014-04-16

    Quantitative trait locus (QTL) mapping studies of Pacific three-spined sticklebacks (Gasterosteus aculeatus) have uncovered several genomic regions controlling variability in different morphological traits, but QTL studies of Atlantic sticklebacks are lacking. We mapped QTL for 40 morphological traits, including body size, body shape, and body armor, in a F2 full-sib cross between northern European marine and freshwater three-spined sticklebacks. A total of 52 significant QTL were identified at the 5% genome-wide level. One major QTL explaining 74.4% of the total variance in lateral plate number was detected on LG4, whereas several major QTL for centroid size (a proxy for body size), and the lengths of two dorsal spines, pelvic spine, and pelvic girdle were mapped on LG21 with the explained variance ranging from 27.9% to 57.6%. Major QTL for landmark coordinates defining body shape variation also were identified on LG21, with each explaining ≥15% of variance in body shape. Multiple QTL for different traits mapped on LG21 overlapped each other, implying pleiotropy and/or tight linkage. Thus, apart from providing confirmatory data to support conclusions born out of earlier QTL studies of Pacific sticklebacks, this study also describes several novel QTL of both major and smaller effect for ecologically important traits. The finding that many major QTL mapped on LG21 suggests that this linkage group might be a hotspot for genetic determinants of ecologically important morphological traits in three-spined sticklebacks.

  15. Effect of natural fibers on mechanical properties of green cement mortar

    NASA Astrophysics Data System (ADS)

    AL-Zubaidi, Aseel B.

    2018-05-01

    Natural fibers of banana, reed, palm and coconut were used to reinforce cement composite. Optical microscopy showed that the prepared fibers are different in size and morphology. Nearly equiaxed, ribbon-like and nearly cylindrical morphologies were observed. Each of the utilized natural fibers was incorporated in the cement matrix at 0, 0.25, 0.5, 0.75 and 1.0 wt% and cured for 28 days. The scanning electron micrographs for the 1.0 wt% -reinforced composite showed differences in porosity, grain size and shape. Each of the utilized fibers has different effect on the microstructure of the cement composite that depends on the fiber size and morphology. Water absorption, thermal conductivity, bending strength, hardness and compression strengths were measured for the reinforced cement composite. It is found that the final physical and mechanical properties of the set cement composite depend on the fiber content and fiber type through the differences in their sizes and morphologies.

  16. Preparation and characterization of shape memory polymer scaffolds via solvent casting/particulate leaching.

    PubMed

    De Nardo, Luigi; Bertoldi, Serena; Cigada, Alberto; Tanzi, Maria Cristina; Haugen, Håvard Jostein; Farè, Silvia

    2012-09-27

    Porous Shape Memory Polymers (SMPs) are ideal candidates for the fabrication of defect fillers, able to support tissue regeneration via minimally invasive approaches. In this regard, control of pore size, shape and interconnection is required to achieve adequate nutrient transport and cell ingrowth. Here, we assessed the feasibility of the preparation of SMP porous structures and characterized their chemico-physical properties and in vitro cell response. SMP scaffolds were obtained via solvent casting/particulate leaching of gelatin microspheres, prepared via oil/water emulsion. A solution of commercial polyether-urethane (MM-4520, Mitsubishi Heavy Industries) was cast on compacted microspheres and leached-off after polymer solvent evaporation. The obtained structures were characterized in terms of morphology (SEM and micro-CT), thermo-mechanical properties (DMTA), shape recovery behavior in compression mode, and in vitro cytocompatibility (MG63 Osteoblast-like cell line). The fabrication process enabled easy control of scaffold morphology, pore size, and pore shape by varying the gelatin microsphere morphology. Homogeneous spherical and interconnected pores have been achieved together with the preservation of shape memory ability, with recovery rate up to 90%. Regardless of pore dimensions, MG63 cells were observed adhering and spreading onto the inner surface of the scaffolds obtained for up to seven days of static in vitro tests. A new class of SMP porous structures has been obtained and tested in vitro: according to these preliminary results reported, SMP scaffolds can be further exploited in the design of a new class of implantable devices.

  17. Particle Morphology Analysis of Biomass Material Based on Improved Image Processing Method

    PubMed Central

    Lu, Zhaolin

    2017-01-01

    Particle morphology, including size and shape, is an important factor that significantly influences the physical and chemical properties of biomass material. Based on image processing technology, a method was developed to process sample images, measure particle dimensions, and analyse the particle size and shape distributions of knife-milled wheat straw, which had been preclassified into five nominal size groups using mechanical sieving approach. Considering the great variation of particle size from micrometer to millimeter, the powders greater than 250 μm were photographed by a flatbed scanner without zoom function, and the others were photographed using a scanning electron microscopy (SEM) with high-image resolution. Actual imaging tests confirmed the excellent effect of backscattered electron (BSE) imaging mode of SEM. Particle aggregation is an important factor that affects the recognition accuracy of the image processing method. In sample preparation, the singulated arrangement and ultrasonic dispersion methods were used to separate powders into particles that were larger and smaller than the nominal size of 250 μm. In addition, an image segmentation algorithm based on particle geometrical information was proposed to recognise the finer clustered powders. Experimental results demonstrated that the improved image processing method was suitable to analyse the particle size and shape distributions of ground biomass materials and solve the size inconsistencies in sieving analysis. PMID:28298925

  18. Go big or go fish: morphological specializations in carnivorous bats.

    PubMed

    Santana, Sharlene E; Cheung, Elena

    2016-05-11

    Specialized carnivory is relatively uncommon across mammals, and bats constitute one of the few groups in which this diet has evolved multiple times. While size and morphological adaptations for carnivory have been identified in other taxa, it is unclear what phenotypic traits characterize the relatively recent evolution of carnivory in bats. To address this gap, we apply geometric morphometric and phylogenetic comparative analyses to elucidate which characters are associated with ecological divergence of carnivorous bats from insectivorous ancestors, and if there is morphological convergence among independent origins of carnivory within bats, and with other carnivorous mammals. We find that carnivorous bats are larger and converged to occupy a subset of the insectivorous morphospace, characterized by skull shapes that enhance bite force at relatively wide gapes. Piscivorous bats are morphologically distinct, with cranial shapes that enable high bite force at narrow gapes, which is necessary for processing fish prey. All animal-eating species exhibit positive allometry in rostrum elongation with respect to skull size, which could allow larger bats to take relatively larger prey. The skull shapes of carnivorous bats share similarities with generalized carnivorans, but tend to be more suited for increased bite force production at the expense of gape, when compared with specialized carnivorans. © 2016 The Author(s).

  19. Go big or go fish: morphological specializations in carnivorous bats

    PubMed Central

    Santana, Sharlene E.; Cheung, Elena

    2016-01-01

    Specialized carnivory is relatively uncommon across mammals, and bats constitute one of the few groups in which this diet has evolved multiple times. While size and morphological adaptations for carnivory have been identified in other taxa, it is unclear what phenotypic traits characterize the relatively recent evolution of carnivory in bats. To address this gap, we apply geometric morphometric and phylogenetic comparative analyses to elucidate which characters are associated with ecological divergence of carnivorous bats from insectivorous ancestors, and if there is morphological convergence among independent origins of carnivory within bats, and with other carnivorous mammals. We find that carnivorous bats are larger and converged to occupy a subset of the insectivorous morphospace, characterized by skull shapes that enhance bite force at relatively wide gapes. Piscivorous bats are morphologically distinct, with cranial shapes that enable high bite force at narrow gapes, which is necessary for processing fish prey. All animal-eating species exhibit positive allometry in rostrum elongation with respect to skull size, which could allow larger bats to take relatively larger prey. The skull shapes of carnivorous bats share similarities with generalized carnivorans, but tend to be more suited for increased bite force production at the expense of gape, when compared with specialized carnivorans. PMID:27170718

  20. [Morphological characterization of annatto fruits 9bixa orellana L.) and its correspondence with protein and isoenzym patterns].

    PubMed

    Medina, A M; Michelangeli, C; Ramis, C; Díaz, A

    2001-01-01

    A group of 32 annatto genotypes collected in five Venezuelan regions (Oriente, Centro, Llanos, Andes and Amazonas) and in Brazil were used for morphological studies. The fruit variables with the greatest discriminatory power in the formation of groups were capsule size, spinosity and seed size. On the other hand, an association group among the variables spinosity, spine length, dehiscence and apex shape were formed, also a proportional association between capsule and seed size, and between dehiscent capsule and brown coloured seeds. Additionally, in order to discriminate morphological variables behaviour in respond to electrophoretic variables, a group of protein and isozyme bands associated with fruit characteristics were established. Therefore, a classification system of this species was possible using morphological studies of the capsules, even though a determined association relating morphological and molecular patterns was not found.

  1. Sex-specific patterns of morphological diversification: evolution of reaction norms and static allometries in neriid flies.

    PubMed

    Cassidy, Elizabeth J; Bath, Eleanor; Chenoweth, Stephen F; Bonduriansky, Russell

    2014-02-01

    The consequences of sex-specific selection for patterns of diversification remain poorly known. Because male secondary sexual traits are typically costly to express, and both costs and benefits are likely to depend on ambient environment and individual condition, such traits may be expected to diversify via changes in reaction norms as well as the scaling of trait size with body size (static allometry). We investigated morphological diversification within two species of Australian neriid flies (Telostylinus angusticollis, Telostylinus lineolatus) by rearing larvae from several populations on larval diets varying sixfold in nutrient concentration. Mean body size varied among populations of T. angusticollis, but body size reaction norms did not vary within either species. However, we detected diversification of reaction norms for body shape in males and females within both species. Moreover, unlike females, males also diversified in static allometry slope and reaction norms for static allometry slope of sexual and nonsexual traits. Our findings reveal qualitative sex differences in patterns of morphological diversification, whereby shape-size relationships diversify extensively in males, but remain conserved in females despite extensive evolution of trait means. Our results highlight the importance of incorporating plasticity and allometry in studies of adaptation and diversification. © 2013 The Author(s). Evolution © 2013 The Society for the Study of Evolution.

  2. Dynamics of nanoparticle morphology under low energy ion irradiation.

    PubMed

    Holland-Moritz, Henry; Graupner, Julia; Möller, Wolfhard; Pacholski, Claudia; Ronning, Carsten

    2018-08-03

    If nanostructures are irradiated with energetic ions, the mechanism of sputtering becomes important when the ion range matches about the size of the nanoparticle. Gold nanoparticles with diameters of ∼50 nm on top of silicon substrates with a native oxide layer were irradiated by gallium ions with energies ranging from 1 to 30 keV in a focused ion beam system. High resolution in situ scanning electron microscopy imaging permits detailed insights in the dynamics of the morphology change and sputter yield. Compared to bulk-like structures or thin films, a pronounced shaping and enhanced sputtering in the nanostructures occurs, which enables a specific shaping of these structures using ion beams. This effect depends on the ratio of nanoparticle size and ion energy. In the investigated energy regime, the sputter yield increases at increasing ion energy and shows a distinct dependence on the nanoparticle size. The experimental findings are directly compared to Monte Carlo simulations obtained from iradina and TRI3DYN, where the latter takes into account dynamic morphological and compositional changes of the target.

  3. Size and habit evolution of PETN crystals - a lattice Monte Carlo study

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zepeda-Ruiz, L A; Maiti, A; Gee, R

    2006-02-28

    Starting from an accurate inter-atomic potential we develop a simple scheme of generating an ''on-lattice'' molecular potential of short range, which is then incorporated into a lattice Monte Carlo code for simulating size and shape evolution of nanocrystallites. As a specific example, we test such a procedure on the morphological evolution of a molecular crystal of interest to us, e.g., Pentaerythritol Tetranitrate, or PETN, and obtain realistic facetted structures in excellent agreement with experimental morphologies. We investigate several interesting effects including, the evolution of the initial shape of a ''seed'' to an equilibrium configuration, and the variation of growth morphologymore » as a function of the rate of particle addition relative to diffusion.« less

  4. Morphological feature extraction for the classification of digital images of cancerous tissues.

    PubMed

    Thiran, J P; Macq, B

    1996-10-01

    This paper presents a new method for automatic recognition of cancerous tissues from an image of a microscopic section. Based on the shape and the size analysis of the observed cells, this method provides the physician with nonsubjective numerical values for four criteria of malignancy. This automatic approach is based on mathematical morphology, and more specifically on the use of Geodesy. This technique is used first to remove the background noise from the image and then to operate a segmentation of the nuclei of the cells and an analysis of their shape, their size, and their texture. From the values of the extracted criteria, an automatic classification of the image (cancerous or not) is finally operated.

  5. How many landmarks are enough to characterize shape and size variation?

    PubMed

    Watanabe, Akinobu

    2018-01-01

    Accurate characterization of morphological variation is crucial for generating reliable results and conclusions concerning changes and differences in form. Despite the prevalence of landmark-based geometric morphometric (GM) data in the scientific literature, a formal treatment of whether sampled landmarks adequately capture shape variation has remained elusive. Here, I introduce LaSEC (Landmark Sampling Evaluation Curve), a computational tool to assess the fidelity of morphological characterization by landmarks. This task is achieved by calculating how subsampled data converge to the pattern of shape variation in the full dataset as landmark sampling is increased incrementally. While the number of landmarks needed for adequate shape variation is dependent on individual datasets, LaSEC helps the user (1) identify under- and oversampling of landmarks; (2) assess robustness of morphological characterization; and (3) determine the number of landmarks that can be removed without compromising shape information. In practice, this knowledge could reduce time and cost associated with data collection, maintain statistical power in certain analyses, and enable the incorporation of incomplete, but important, specimens to the dataset. Results based on simulated shape data also reveal general properties of landmark data, including statistical consistency where sampling additional landmarks has the tendency to asymptotically improve the accuracy of morphological characterization. As landmark-based GM data become more widely adopted, LaSEC provides a systematic approach to evaluate and refine the collection of shape data--a goal paramount for accumulation and analysis of accurate morphological information.

  6. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chuang, Chihpin; Singh, Dileep; Kenesei, Peter

    The size and morphology of the graphite particles play a crucial role in determining various mechanical and thermal properties of cast iron. In the present study, we utilized high-energy synchrotron X-ray tomography to perform quantitative 3D-characterization of the distribution of graphite particles in high-strength compacted graphite iron (CGI). The size, shape, and spatial connectivity of graphite were examined. The analysis reveals that the compacted graphite can grow with a coral-tree-like morphology and span several hundred microns in the iron matrix.

  7. Energetic benefits and adaptations in mammalian limbs: Scale effects and selective pressures.

    PubMed

    Kilbourne, Brandon M; Hoffman, Louwrens C

    2015-06-01

    Differences in limb size and shape are fundamental to mammalian morphological diversity; however, their relevance to locomotor costs has long been subject to debate. In particular, it remains unknown if scale effects in whole limb morphology could partially underlie decreasing mass-specific locomotor costs with increasing limb length. Whole fore- and hindlimb inertial properties reflecting limb size and shape-moment of inertia (MOI), mass, mass distribution, and natural frequency-were regressed against limb length for 44 species of quadrupedal mammals. Limb mass, MOI, and center of mass position are negatively allometric, having a strong potential for lowering mass-specific locomotor costs in large terrestrial mammals. Negative allometry of limb MOI results in a 40% reduction in MOI relative to isometry's prediction for our largest sampled taxa. However, fitting regression residuals to adaptive diversification models reveals that codiversification of limb mass, limb length, and body mass likely results from selection for differing locomotor modes of running, climbing, digging, and swimming. The observed allometric scaling does not result from selection for energetically beneficial whole limb morphology with increasing size. Instead, our data suggest that it is a consequence of differing morphological adaptations and body size distributions among quadrupedal mammals, highlighting the role of differing limb functions in mammalian evolution. © 2015 The Author(s). Evolution © 2015 The Society for the Study of Evolution.

  8. Morphologic and proteomic characterization of exosomes released by cultured extravillous trophoblast cells

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Atay, Safinur; Gercel-Taylor, Cicek; Kesimer, Mehmet

    Exosomes represent an important intercellular communication vehicle, mediating events essential for the decidual microenvironment. While we have demonstrated exosome induction of pro-inflammatory cytokines, to date, no extensive characterization of trophoblast-derived exosomes has been provided. Our objective was to provide a morphologic and proteomic characterization of these exosomes. Exosomes were isolated from the conditioned media of Swan71 human trophoblast cells by ultrafiltration and ultracentrifugation. These were analyzed for density (sucrose density gradient centrifugation), morphology (electron microscopy), size (dynamic light scattering) and protein composition (Ion Trap mass spectrometry and western immunoblotting). Based on density gradient centrifugation, microvesicles from Sw71 cells exhibit amore » density between 1.134 and 1.173 g/ml. Electron microscopy demonstrated that microvesicles from Sw71 cells exhibit the characteristic cup-shaped morphology of exosomes. Dynamic light scattering showed a bell-shaped curve, indicating a homogeneous population with a mean size of 165 nm {+-} 0.5 nm. Ion Trap mass spectrometry demonstrated the presence of exosome marker proteins (including CD81, Alix, cytoskeleton related proteins, and Rab family). The MS results were confirmed by western immunoblotting. Based on morphology, density, size and protein composition, we defined the release of exosomes from extravillous trophoblast cells and provide their first extensive characterization. This characterization is essential in furthering our understanding of 'normal' early pregnancy.« less

  9. Morphological variation in Sarracenia purpurea (Sarraceniaceae): geographic, environmental, and taxonomic correlates.

    PubMed

    Ellison, Aaron M; Buckley, Hannah L; Miller, Thomas E; Gotelli, Nicholas J

    2004-11-01

    Geographic variation in morphology reflects phenotypic responses to environmental gradients and evolutionary history of populations and species and may indicate local or regional changes in environmental conditions. The pitcher plant (Sarracenia purpurea) illustrates these principles. At local scales, its morphology reflects nutrient availability. At points along its broad geographic range (from Florida to northern Canada) morphology has been used to distinguish subspecies and varieties, but there has been no detailed study of the continuum of morphological variation across this entire range. Patterns of morphological variation in S. purpurea were characterized as a function of climatic and environmental conditions at 39 sites spanning its range. Differences in pitcher size and shape were strongly correlated with temperature, annual precipitation, and availability of ammonium and calcium in peat pore water. Pitcher shape (lip width, mouth diameter, and pitcher width) in Florida panhandle populations differed significantly from pitcher shape of all other populations, even after accounting for environmental correlations. In contrast, the northern and southern subspecies of S. purpurea (the latter exclusive of the Florida panhandle populations) cannot be distinguished based on these morphological measurements alone. These results support a recent proposal that identifies the Florida populations as a distinct species, Sarracenia rosea.

  10. The effect of particle morphology on the physical stability of pharmaceutical powder mixtures

    NASA Astrophysics Data System (ADS)

    Swaminathan, Vidya

    Pharmaceutical powder mixtures are composed of particles that physically interact, precluding the formation of random mixtures. Mixtures based on particle interactions are termed ordered mixtures. The objective of this study was to determine the effect of the morphological characteristics of the components, surface texture and shape, along with size, on the formation of stable mixtures. Morphological parameters were obtained from image analysis measurements. Surface roughness was quantified using the ratio of the perimeter of the particle to that of an ideal shape (circle or square) having the same area; shape was described using the aspect ratio. The stability of mixtures of micronized aspirin with carriers of different surface roughness was determined by measuring the extent of drug adhering to the carrier after subjecting the mixtures to vibration. A lesser extent of segregation of drug from highly textured carriers relative to smoother textured carriers was observed. This was postulated to be due to a larger concentration of surface asperities on the coarser carriers which constitute potentially strong adhesion sites. The electrostatic charge on the powders was measured; differences in the response of the mixtures to the addition of magnesium stearate were attributed to electrostatic charge effects. The effect of varying the aspect ratio of the carrier and drug on segregation in polydisperse mixtures was determined from the coefficient of variation of the drug in the mixture as a function of mixing time. Reducing the size of the carrier resulted in poor homogeneity due to weak carrier-drug interactions. The variation in drug content resulting from a change in the shape of the carriers was smaller than that caused by size differences. The segregation rate constant in mixtures having dissimilarly shaped components was larger than in mixtures having components of similar shape. The effects of magnesium stearate concentration and lubrication time on the content uniformity of polydisperse mixtures were evaluated from a full factorial experiment. The segregation response of ordered and random mixtures to the addition of magnesium stearate was compared. The moisture sorption behavior of commercial magnesium stearate and the resulting morphological changes were evaluated.

  11. Leaf Morphology, Taxonomy and Geometric Morphometrics: A Simplified Protocol for Beginners

    PubMed Central

    Viscosi, Vincenzo; Cardini, Andrea

    2011-01-01

    Taxonomy relies greatly on morphology to discriminate groups. Computerized geometric morphometric methods for quantitative shape analysis measure, test and visualize differences in form in a highly effective, reproducible, accurate and statistically powerful way. Plant leaves are commonly used in taxonomic analyses and are particularly suitable to landmark based geometric morphometrics. However, botanists do not yet seem to have taken advantage of this set of methods in their studies as much as zoologists have done. Using free software and an example dataset from two geographical populations of sessile oak leaves, we describe in detailed but simple terms how to: a) compute size and shape variables using Procrustes methods; b) test measurement error and the main levels of variation (population and trees) using a hierachical design; c) estimate the accuracy of group discrimination; d) repeat this estimate after controlling for the effect of size differences on shape (i.e., allometry). Measurement error was completely negligible; individual variation in leaf morphology was large and differences between trees were generally bigger than within trees; differences between the two geographic populations were small in both size and shape; despite a weak allometric trend, controlling for the effect of size on shape slighly increased discrimination accuracy. Procrustes based methods for the analysis of landmarks were highly efficient in measuring the hierarchical structure of differences in leaves and in revealing very small-scale variation. In taxonomy and many other fields of botany and biology, the application of geometric morphometrics contributes to increase scientific rigour in the description of important aspects of the phenotypic dimension of biodiversity. Easy to follow but detailed step by step example studies can promote a more extensive use of these numerical methods, as they provide an introduction to the discipline which, for many biologists, is less intimidating than the often inaccessible specialistic literature. PMID:21991324

  12. A Geographic Cline of Skull and Brain Morphology among Individuals of European Ancestry

    PubMed Central

    Bakken, Trygve E.; Dale, Anders M.; Schork, Nicholas J.

    2011-01-01

    Background Human skull and brain morphology are strongly influenced by genetic factors, and skull size and shape vary worldwide. However, the relationship between specific brain morphology and genetically-determined ancestry is largely unknown. Methods We used two independent data sets to characterize variation in skull and brain morphology among individuals of European ancestry. The first data set is a historical sample of 1,170 male skulls with 37 shape measurements drawn from 27 European populations. The second data set includes 626 North American individuals of European ancestry participating in the Alzheimer's Disease Neuroimaging Initiative (ADNI) with magnetic resonance imaging, height and weight, neurological diagnosis, and genome-wide single nucleotide polymorphism (SNP) data. Results We found that both skull and brain morphological variation exhibit a population-genetic fingerprint among individuals of European ancestry. This fingerprint shows a Northwest to Southeast gradient, is independent of body size, and involves frontotemporal cortical regions. Conclusion Our findings are consistent with prior evidence for gene flow in Europe due to historical population movements and indicate that genetic background should be considered in studies seeking to identify genes involved in human cortical development and neuropsychiatric disease. PMID:21849792

  13. Correlates across the Structural, Functional, and Molecular Phenotypes of Fragile X Syndrome

    ERIC Educational Resources Information Center

    Beckel-Mitchener, Andrea; Greenough, William T.

    2004-01-01

    Fragile X syndrome (FXS) is characterized by a pattern of morphological, functional, and molecular characteristics with, in at least some cases, apparent relationships among phenotypic features at different levels. Gross morphology differences in the sizes of some human brain regions are accompanied by fine structural alterations in the shapes and…

  14. Identification of Major and Minor QTL for Ecologically Important Morphological Traits in Three-Spined Sticklebacks (Gasterosteus aculeatus)

    PubMed Central

    Liu, Jun; Shikano, Takahito; Leinonen, Tuomas; Cano, José Manuel; Li, Meng-Hua; Merilä, Juha

    2014-01-01

    Quantitative trait locus (QTL) mapping studies of Pacific three-spined sticklebacks (Gasterosteus aculeatus) have uncovered several genomic regions controlling variability in different morphological traits, but QTL studies of Atlantic sticklebacks are lacking. We mapped QTL for 40 morphological traits, including body size, body shape, and body armor, in a F2 full-sib cross between northern European marine and freshwater three-spined sticklebacks. A total of 52 significant QTL were identified at the 5% genome-wide level. One major QTL explaining 74.4% of the total variance in lateral plate number was detected on LG4, whereas several major QTL for centroid size (a proxy for body size), and the lengths of two dorsal spines, pelvic spine, and pelvic girdle were mapped on LG21 with the explained variance ranging from 27.9% to 57.6%. Major QTL for landmark coordinates defining body shape variation also were identified on LG21, with each explaining ≥15% of variance in body shape. Multiple QTL for different traits mapped on LG21 overlapped each other, implying pleiotropy and/or tight linkage. Thus, apart from providing confirmatory data to support conclusions born out of earlier QTL studies of Pacific sticklebacks, this study also describes several novel QTL of both major and smaller effect for ecologically important traits. The finding that many major QTL mapped on LG21 suggests that this linkage group might be a hotspot for genetic determinants of ecologically important morphological traits in three-spined sticklebacks. PMID:24531726

  15. Bulk and monolayer ordering of block copolymer blends

    NASA Astrophysics Data System (ADS)

    Onikoyi, Adetunji J.

    The control of the nanoscale structure or morphology of a block copolymer is a desired goal for nanolithography applications. In this work, we are particularly interested in providing guides for controlling domain size, domain shape and defect densities in block copolymers and their blends for thin film applications. To reach this goal, a sphere forming PS-b-P2VP (having a PS majority block) and its blends with PS homopolymer or cylinder forming PS-b-P2VP are studied in both the bulk and thin films. Structure characterization is performed using a variety of experimental techniques including small angle X-ray scattering, scanning force microscopy and transmission electron microscopy. In the bulk, the spherical domains of the pure, sphere forming PS-b-P2VP arrange on a BCC lattice. On adding PS homopolymer (hPS), the lattice parameter of the BCC spheres increases, while the order-to-disorder temperature (ODT) of the BCC lattice simultaneously decreases. At a given hPS composition, the use of larger sized hPS leads to larger increases in the lattice parameter and larger decreases in the ODT. In bulk blends of cylinder forming PS-b-P2VP with sphere forming PS-b-P2VP, the ordered morphology changes (e.g., cylindrical morphology → coexisting spherical and cylindrical morphologies → spherical morphology) as the sphere forming PS-b-P2VP volume fraction phis increases, while the ODT of the cylindrical morphology decreases. The phase boundaries of these morphologies in monolayers shift to lower phis compared to those of the bulk, apparently caused by a selective adsorption of the cylindrical PS-b-P2VP to form a brush on the substrate. This selective adsorption leads to a preference for spherical domains in diamond-shaped lateral confinements when cylindrical domains are stabilized outside the confinements on the same substrate. Finally, we explore the use of graphoepitaxy to order monolayers of sphere forming PS-b-P2VP and its blends with hPS. The probability of forming isolated dislocations, or of adding (or removing) a full row of spherical domains, in diamond-shaped lateral confinements is shown to be higher when the well size is incommensurate with the lattice parameter. Square-shaped lateral confinement leads to a preference for square sphere packing if the PS-b-P2VP is blended with appropriate amounts of hPS.

  16. 3D granulometry: grain-scale shape and size distribution from point cloud dataset of river environments

    NASA Astrophysics Data System (ADS)

    Steer, Philippe; Lague, Dimitri; Gourdon, Aurélie; Croissant, Thomas; Crave, Alain

    2016-04-01

    The grain-scale morphology of river sediments and their size distribution are important factors controlling the efficiency of fluvial erosion and transport. In turn, constraining the spatial evolution of these two metrics offer deep insights on the dynamics of river erosion and sediment transport from hillslopes to the sea. However, the size distribution of river sediments is generally assessed using statistically-biased field measurements and determining the grain-scale shape of river sediments remains a real challenge in geomorphology. Here we determine, with new methodological approaches based on the segmentation and geomorphological fitting of 3D point cloud dataset, the size distribution and grain-scale shape of sediments located in river environments. Point cloud segmentation is performed using either machine-learning algorithms or geometrical criterion, such as local plan fitting or curvature analysis. Once the grains are individualized into several sub-clouds, each grain-scale morphology is determined using a 3D geometrical fitting algorithm applied on the sub-cloud. If different geometrical models can be conceived and tested, only ellipsoidal models were used in this study. A phase of results checking is then performed to remove grains showing a best-fitting model with a low level of confidence. The main benefits of this automatic method are that it provides 1) an un-biased estimate of grain-size distribution on a large range of scales, from centimeter to tens of meters; 2) access to a very large number of data, only limited by the number of grains in the point-cloud dataset; 3) access to the 3D morphology of grains, in turn allowing to develop new metrics characterizing the size and shape of grains. The main limit of this method is that it is only able to detect grains with a characteristic size greater than the resolution of the point cloud. This new 3D granulometric method is then applied to river terraces both in the Poerua catchment in New-Zealand and along the Laonong river in Taiwan, which point clouds were obtained using both terrestrial lidar scanning and structure from motion photogrammetry.

  17. Shape, size, and maturity trajectories of the human ilium.

    PubMed

    Wilson, Laura A B; Ives, Rachel; Cardoso, Hugo F V; Humphrey, Louise T

    2015-01-01

    Morphological traits of the ilium have consistently been more successful for juvenile sex determination than have techniques applied to other skeletal elements, however relatively little is known about the ontogeny and maturation of size and shape dimorphism in the ilium. We use a geometric morphometric approach to quantitatively separate the ontogeny of size and shape of the ilium, and analyze interpopulation differences in the onset, rate and patterning of sexual dimorphism. We captured the shape of three traits for a total of 191 ilia from Lisbon (Portugal) and London (UK) samples of known age and sex (0-17 years). Our results indicate that a) there is a clear dissociation between the ontogeny of size and shape in males and females, b) the ontogeny of size and shape are each defined by non-linear trajectories that differ between the sexes, c) there are interpopulation differences in ontogenetic shape trajectories, which point to population-specific patterning in the attainment of sexual dimorphism, and d) the rate of shape maturation and size maturation is typically higher for females than males. Male and female shape differences in the ilium are brought about by trajectory divergence. Differences in size and shape maturation between the sexes suggest that maturity may confound our ability to discriminate between the sexes by introducing variation not accounted for in age-based groupings. The accuracy of sex determination methods using the ilium may be improved by the use of different traits for particular age groups, to capture the ontogenetic development of shape in both sexes. © 2014 Wiley Periodicals, Inc.

  18. Moderate climate signature in cranial anatomy of late holocene human populations from Southern South America.

    PubMed

    Paula Menéndez, Lumila

    2018-02-01

    The aim of this study is to analyze the association between cranial variation and climate in order to discuss their role during the diversification of southern South American populations. Therefore, the specific objectives are: (1) to explore the spatial pattern of cranial variation with regard to the climatic diversity of the region, and (2) to evaluate the differential impact that the climatic factors may have had on the shape and size of the diverse cranial structures studied. The variation in shape and size of 361 crania was studied, registering 62 3D landmarks that capture shape and size variation in the face, cranial vault, and base. Mean, minimum, and maximum annual temperature, as well as mean annual precipitation, but also diet and altitude, were matched for each population sample. A PCA, as well as spatial statistical techniques, including kriging, regression, and multimodel inference were employed. The facial skeleton size presents a latitudinal pattern which is partially associated with temperature diversity. Both diet and altitude are the variables that mainly explain the skull shape variation, although mean annual temperature also plays a role. The association between climate factors and cranial variation is low to moderate, mean annual temperature explains almost 40% of the entire skull, facial skeleton and cranial vault shape variation, while annual precipitation and minimum annual temperature only contribute to the morphological variation when considered together with maximum annual temperature. The cranial base is the structure less associated with climate diversity. These results suggest that climate factors may have had a partial impact on the facial and vault shape, and therefore contributed moderately to the diversification of southern South American populations, while diet and altitude might have had a stronger impact. Therefore, cranial variation at the southern cone has been shaped both by random and nonrandom factors. Particularly, the influence of climate on skull shape has probably been the result of directional selection. This study supports that, although cranial vault is the cranial structure more associated to mean annual temperature, the impact of climate signature on morphology decreases when populations from extreme cold environments are excluded from the analysis. Additionally, it shows that the extent of the geographical scales analyzed, as well as differential sampling may lead to different results regarding the role of ecological factors and evolutionary processes on cranial morphology. © 2017 Wiley Periodicals, Inc.

  19. Morphological Evolution of Block Copolymer Particles: Effect of Solvent Evaporation Rate on Particle Shape and Morphology.

    PubMed

    Shin, Jae Man; Kim, YongJoo; Yun, Hongseok; Yi, Gi-Ra; Kim, Bumjoon J

    2017-02-28

    Shape and morphology of polymeric particles are of great importance in controlling their optical properties or self-assembly into unusual superstructures. Confinement of block copolymers (BCPs) in evaporative emulsions affords particles with diverse structures, including prolate ellipsoids, onion-like spheres, oblate ellipsoids, and others. Herein, we report that the evaporation rate of solvent from emulsions encapsulating symmetric polystyrene-b-polybutadiene (PS-b-PB) determines the shape and internal nanostructure of micron-sized BCP particles. A distinct morphological transition from the ellipsoids with striped lamellae to the onion-like spheres was observed with decreasing evaporation rate. Experiments and dissipative particle dynamics (DPD) simulations showed that the evaporation rate affected the organization of BCPs at the particle surface, which determined the final shape and internal nanostructure of the particles. Differences in the solvent diffusion rates in PS and PB at rapid evaporation rates induced alignment of both domains perpendicular to the particle surface, resulting in ellipsoids with axial lamellar stripes. Slower evaporation rates provided sufficient time for BCP organization into onion-like structures with PB as the outermost layer, owing to the preferential interaction of PB with the surroundings. BCP molecular weight was found to influence the critical evaporation rate corresponding to the morphological transition from ellipsoid to onion-like particles, as well as the ellipsoid aspect ratio. DPD simulations produced morphologies similar to those obtained from experiments and thus elucidated the mechanism and driving forces responsible for the evaporation-induced assembly of BCPs into particles with well-defined shapes and morphologies.

  20. Do size, shape, and alignment parameters of the femoral condyle affect the trochlear groove tracking? A morphometric study based on 3D- computed tomography models in Chinese people.

    PubMed

    Du, Zhe; Chen, Shichang; Yan, Mengning; Yue, Bing; Zeng, Yiming; Wang, You

    2017-01-06

    Our study aimed to investigate whether geometrical features (size, shape, or alignment parameters) of the femoral condyle affect the morphology of the trochlear groove. Computed tomography models of 195 femurs (97 and 98 knees from male and female subjects, respectively) were reconstructed into three-dimensional models and categorised into four types of trochlear groove morphology based on the position of the turning point in relation to the mechanical axis (types 45°, 60°, 75°, and 90°). Only subjects with healthy knees were included, whereas individuals with previous knee trauma or knee pain, soft tissue injury, osteoarthritis, or other chronic diseases of the musculoskeletal system were excluded. The size parameters were: radius of the best-fit cylinder, anteroposterior dimension of the lateral condyles (AP), and distal mediolateral dimension (ML). The shape parameters were: aspect ratio (AP/ML), arc angle, and proximal- and distal- end angles. The alignment parameters were: knee valgus physiologic angle (KVPA), mechanical medial distal femoral angle (mMDFA), and hip-knee-ankle angle (HKA). All variables were measured in the femoral condyle models, and the means for each groove type were compared using one-way analysis of variance. No significant difference among groove types was observed regarding size parameters. There were significant differences when comparing type 45° with types 60°, 75°, and 90° regarding aspect ratio and distal-end angle (p < 0.05), but not regarding proximal-end angle. There were significant differences when comparing type 90° with types 45°, 60°, and 75° regarding KVPA, mMDFA, and HKA (p < 0.05). Among size, shape, and alignment parameters, the latter two exhibited partial influence on the morphology of the trochlear groove. Shape parameters affected the trochlear groove for trochlear type 45°, for which the femoral condyle was relatively flat, whereas alignment parameters affected the trochlear groove for trochlear type 90°, showing that knees in type 90° tend to be valgus. The morphometric analysis based on trochlear groove classification may be helpful for the future design of individualized prostheses.

  1. Investigation of the Evolution of Crystal Size and Shape during Temperature Cycling and in the Presence of a Polymeric Additive Using Combined Process Analytical Technologies

    PubMed Central

    2017-01-01

    Crystal size and shape can be manipulated to enhance the qualities of the final product. In this work the steady-state shape and size of succinic acid crystals, with and without a polymeric additive (Pluronic P123) at 350 mL, scale is reported. The effect of the amplitude of cycles as well as the heating/cooling rates is described, and convergent cycling (direct nucleation control) is compared to static cycling. The results show that the shape of succinic acid crystals changes from plate- to diamond-like after multiple cycling steps, and that the time required for this morphology change to occur is strongly related to the type of cycling. Addition of the polymer is shown to affect both the final shape of the crystals and the time needed to reach size and shape steady-state conditions. It is shown how this phenomenon can be used to improve the design of the crystallization step in order to achieve more efficient downstream operations and, in general, to help optimize the whole manufacturing process. PMID:28867966

  2. Bat Species Comparisons Based on External Morphology: A Test of Traditional versus Geometric Morphometric Approaches

    PubMed Central

    Schmieder, Daniela A.; Benítez, Hugo A.; Borissov, Ivailo M.; Fruciano, Carmelo

    2015-01-01

    External morphology is commonly used to identify bats as well as to investigate flight and foraging behavior, typically relying on simple length and area measures or ratios. However, geometric morphometrics is increasingly used in the biological sciences to analyse variation in shape and discriminate among species and populations. Here we compare the ability of traditional versus geometric morphometric methods in discriminating between closely related bat species – in this case European horseshoe bats (Rhinolophidae, Chiroptera) – based on morphology of the wing, body and tail. In addition to comparing morphometric methods, we used geometric morphometrics to detect interspecies differences as shape changes. Geometric morphometrics yielded improved species discrimination relative to traditional methods. The predicted shape for the variation along the between group principal components revealed that the largest differences between species lay in the extent to which the wing reaches in the direction of the head. This strong trend in interspecific shape variation is associated with size, which we interpret as an evolutionary allometry pattern. PMID:25965335

  3. Morphological Evolution of Pit-Patterned Si(001) Substrates Driven by Surface-Energy Reduction

    NASA Astrophysics Data System (ADS)

    Salvalaglio, Marco; Backofen, Rainer; Voigt, Axel; Montalenti, Francesco

    2017-09-01

    Lateral ordering of heteroepitaxial islands can be conveniently achieved by suitable pit-patterning of the substrate prior to deposition. Controlling shape, orientation, and size of the pits is not trivial as, being metastable, they can significantly evolve during deposition/annealing. In this paper, we exploit a continuum model to explore the typical metastable pit morphologies that can be expected on Si(001), depending on the initial depth/shape. Evolution is predicted using a surface-diffusion model, formulated in a phase-field framework, and tackling surface-energy anisotropy. Results are shown to nicely reproduce typical metastable shapes reported in the literature. Moreover, long time scale evolutions of pit profiles with different depths are found to follow a similar kinetic pathway. The model is also exploited to treat the case of heteroepitaxial growth involving two materials characterized by different facets in their equilibrium Wulff's shape. This can lead to significant changes in morphologies, such as a rotation of the pit during deposition as evidenced in Ge/Si experiments.

  4. Morphological Evolution of Pit-Patterned Si(001) Substrates Driven by Surface-Energy Reduction.

    PubMed

    Salvalaglio, Marco; Backofen, Rainer; Voigt, Axel; Montalenti, Francesco

    2017-09-29

    Lateral ordering of heteroepitaxial islands can be conveniently achieved by suitable pit-patterning of the substrate prior to deposition. Controlling shape, orientation, and size of the pits is not trivial as, being metastable, they can significantly evolve during deposition/annealing. In this paper, we exploit a continuum model to explore the typical metastable pit morphologies that can be expected on Si(001), depending on the initial depth/shape. Evolution is predicted using a surface-diffusion model, formulated in a phase-field framework, and tackling surface-energy anisotropy. Results are shown to nicely reproduce typical metastable shapes reported in the literature. Moreover, long time scale evolutions of pit profiles with different depths are found to follow a similar kinetic pathway. The model is also exploited to treat the case of heteroepitaxial growth involving two materials characterized by different facets in their equilibrium Wulff's shape. This can lead to significant changes in morphologies, such as a rotation of the pit during deposition as evidenced in Ge/Si experiments.

  5. Size and shape measurement in contemporary cephalometrics.

    PubMed

    McIntyre, Grant T; Mossey, Peter A

    2003-06-01

    The traditional method of analysing cephalograms--conventional cephalometric analysis (CCA)--involves the calculation of linear distance measurements, angular measurements, area measurements, and ratios. Because shape information cannot be determined from these 'size-based' measurements, an increasing number of studies employ geometric morphometric tools in the cephalometric analysis of craniofacial morphology. Most of the discussions surrounding the appropriateness of CCA, Procrustes superimposition, Euclidean distance matrix analysis (EDMA), thin-plate spline analysis (TPS), finite element morphometry (FEM), elliptical Fourier functions (EFF), and medial axis analysis (MAA) have centred upon mathematical and statistical arguments. Surprisingly, little information is available to assist the orthodontist in the clinical relevance of each technique. This article evaluates the advantages and limitations of the above methods currently used to analyse the craniofacial morphology on cephalograms and investigates their clinical relevance and possible applications.

  6. Body size and allometric variation in facial shape in children.

    PubMed

    Larson, Jacinda R; Manyama, Mange F; Cole, Joanne B; Gonzalez, Paula N; Percival, Christopher J; Liberton, Denise K; Ferrara, Tracey M; Riccardi, Sheri L; Kimwaga, Emmanuel A; Mathayo, Joshua; Spitzmacher, Jared A; Rolian, Campbell; Jamniczky, Heather A; Weinberg, Seth M; Roseman, Charles C; Klein, Ophir; Lukowiak, Ken; Spritz, Richard A; Hallgrimsson, Benedikt

    2018-02-01

    Morphological integration, or the tendency for covariation, is commonly seen in complex traits such as the human face. The effects of growth on shape, or allometry, represent a ubiquitous but poorly understood axis of integration. We address the question of to what extent age and measures of size converge on a single pattern of allometry for human facial shape. Our study is based on two large cross-sectional cohorts of children, one from Tanzania and the other from the United States (N = 7,173). We employ 3D facial imaging and geometric morphometrics to relate facial shape to age and anthropometric measures. The two populations differ significantly in facial shape, but the magnitude of this difference is small relative to the variation within each group. Allometric variation for facial shape is similar in both populations, representing a small but significant proportion of total variation in facial shape. Different measures of size are associated with overlapping but statistically distinct aspects of shape variation. Only half of the size-related variation in facial shape can be explained by the first principal component of four size measures and age while the remainder associates distinctly with individual measures. Allometric variation in the human face is complex and should not be regarded as a singular effect. This finding has important implications for how size is treated in studies of human facial shape and for the developmental basis for allometric variation more generally. © 2017 Wiley Periodicals, Inc.

  7. Tuning ligament shape in dealloyed nanoporous tin and the impact of nanoscale morphology on its applications in Na-ion alloy battery anodes

    NASA Astrophysics Data System (ADS)

    Detsi, Eric; Petrissans, Xavier; Yan, Yan; Cook, John B.; Deng, Ziling; Liang, Yu-Lun; Dunn, Bruce; Tolbert, Sarah H.

    2018-05-01

    Control over the morphology of nanostructured materials is of primary importance in structure-property relationship studies. Although the size of ligaments and pores in dealloyed nanoporous metals can be controlled by thermal and/or (electro)chemical treatments, tuning the shape of those ligaments is much harder. In the present work, we use corroding media with different reactivity to effectively tailor the ligament shape in nanoporous tin (NP-Sn) during dealloying by free corrosion. NP-Sn architectures with nanowire and granular ligament shapes were made by controlling the pH of the corroding solution, and thus the rate of Sn oxidation relative to the etching rate of the sacrificial component. The standard nanowire structure was formed under acidic conditions where oxidation was slow, but a hierarchical granular structure was formed when fusion of the Sn nanocrystals was inhibited by surface oxidation. To demonstrate the advantages of this architectural control, these two materials systems were investigated as electrodes for Na-ion battery anodes. Similar initial Na storage capacities of ˜500 and 550 mAh/g were achieved in the nanowire and granular materials, respectively, but the cycle life of the two materials was quite different. NP-Sn with a granular ligament shape showed enhanced stability with a capacity retention of ˜55 % over 95 cycles at a specific current of 40 mA/g. By contrast, NP-Sn with a nanowire ligament shape showed very fast capacity fading within the first 10 cycles. This work thus demonstrates the dramatic impact of the nanoscale morphology on the electrochemical performance of nanoporous materials and highlights the need for both shape and size control in dealloyed nanoporous metals.

  8. Morphological evolution, ecological diversification and climate change in rodents.

    PubMed

    Renaud, Sabrina; Michaux, Jacques; Schmidt, Daniela N; Aguilar, Jean-Pierre; Mein, Pierre; Auffray, Jean-Christophe

    2005-03-22

    Among rodents, the lineage from Progonomys hispanicus to Stephanomys documents a case of increasing size and dental specialization during an approximately 9 Myr time-interval. On the contrary, some contemporaneous generalist lineages like Apodemus show a limited morphological evolution. Dental shape can be related to diet and can be used to assess the ecological changes along the lineages. Consequently, size and shape of the first upper molar were measured in order to quantify the patterns of morphological evolution along both lineages and compare them to environmental trends. Climatic changes do not have a direct influence on evolution, but they open new ecological opportunities by changing vegetation and allow the evolution of a specialist like Stephanomys. On the other hand, environmental changes are not dramatic enough to destroy the habitat of a long-term generalist like Apodemus. Hence, our results exemplify a case of an influence of climate on the evolution of specialist species, although a generalist species may persist without change.

  9. Morphological evolution, ecological diversification and climate change in rodents

    PubMed Central

    Renaud, Sabrina; Michaux, Jacques; Schmidt, Daniela N; Aguilar, Jean-Pierre; Mein, Pierre; Auffray, Jean-Christophe

    2005-01-01

    Among rodents, the lineage from Progonomys hispanicus to Stephanomys documents a case of increasing size and dental specialization during an approximately 9 Myr time-interval. On the contrary, some contemporaneous generalist lineages like Apodemus show a limited morphological evolution. Dental shape can be related to diet and can be used to assess the ecological changes along the lineages. Consequently, size and shape of the first upper molar were measured in order to quantify the patterns of morphological evolution along both lineages and compare them to environmental trends. Climatic changes do not have a direct influence on evolution, but they open new ecological opportunities by changing vegetation and allow the evolution of a specialist like Stephanomys. On the other hand, environmental changes are not dramatic enough to destroy the habitat of a long-term generalist like Apodemus. Hence, our results exemplify a case of an influence of climate on the evolution of specialist species, although a generalist species may persist without change. PMID:15817435

  10. Fabrication of ordered arrays of micro- and nanoscale features with control over their shape and size via templated solid-state dewetting.

    PubMed

    Ye, Jongpil

    2015-05-08

    Templated solid-state dewetting of single-crystal films has been shown to be used to produce regular patterns of various shapes. However, the materials for which this patterning method is applicable, and the size range of the patterns produced are still limited. Here, it is shown that ordered arrays of micro- and nanoscale features can be produced with control over their shape and size via solid-state dewetting of patches patterned from single-crystal palladium and nickel films of different thicknesses and orientations. The shape and size characteristics of the patterns are found to be widely controllable with varying the shape, width, thickness, and orientation of the initial patches. The morphological evolution of the patches is also dependent on the film material, with different dewetting behaviors observed in palladium and nickel films. The mechanisms underlying the pattern formation are explained in terms of the influence on Rayleigh-like instability of the patch geometry and the surface energy anisotropy of the film material. This mechanistic understanding of pattern formation can be used to design patches for the precise fabrication of micro- and nanoscale structures with the desired shapes and feature sizes.

  11. Fabrication of ordered arrays of micro- and nanoscale features with control over their shape and size via templated solid-state dewetting

    PubMed Central

    Ye, Jongpil

    2015-01-01

    Templated solid-state dewetting of single-crystal films has been shown to be used to produce regular patterns of various shapes. However, the materials for which this patterning method is applicable, and the size range of the patterns produced are still limited. Here, it is shown that ordered arrays of micro- and nanoscale features can be produced with control over their shape and size via solid-state dewetting of patches patterned from single-crystal palladium and nickel films of different thicknesses and orientations. The shape and size characteristics of the patterns are found to be widely controllable with varying the shape, width, thickness, and orientation of the initial patches. The morphological evolution of the patches is also dependent on the film material, with different dewetting behaviors observed in palladium and nickel films. The mechanisms underlying the pattern formation are explained in terms of the influence on Rayleigh-like instability of the patch geometry and the surface energy anisotropy of the film material. This mechanistic understanding of pattern formation can be used to design patches for the precise fabrication of micro- and nanoscale structures with the desired shapes and feature sizes. PMID:25951816

  12. Effects of laser power density and initial grain size in laser shock punching of pure copper foil

    NASA Astrophysics Data System (ADS)

    Zheng, Chao; Zhang, Xiu; Zhang, Yiliang; Ji, Zhong; Luan, Yiguo; Song, Libin

    2018-06-01

    The effects of laser power density and initial grain size on forming quality of holes in laser shock punching process were investigated in the present study. Three different initial grain sizes as well as three levels of laser power densities were provided, and then laser shock punching experiments of T2 copper foil were conducted. Based upon the experimental results, the characteristics of shape accuracy, fracture surface morphology and microstructures of punched holes were examined. It is revealed that the initial grain size has a noticeable effect on forming quality of holes punched by laser shock. The shape accuracy of punched holes degrades with the increase of grain size. As the laser power density is enhanced, the shape accuracy can be improved except for the case in which the ratio of foil thickness to initial grain size is approximately equal to 1. Compared with the fracture surface morphology in the quasistatic loading conditions, the fracture surface after laser shock can be divided into three zones including rollover, shearing and burr. The distribution of the above three zones strongly relates with the initial grain size. When the laser power density is enhanced, the shearing depth is not increased, but even diminishes in some cases. There is no obvious change of microstructures with the enhancement of laser power density. However, while the initial grain size is close to the foil thickness, single-crystal shear deformation may occur, suggesting that the ratio of foil thickness to initial grain size has an important impact on deformation behavior of metal foil in laser shock punching process.

  13. Three-dimensional quantitative analysis of healthy foot shape: a proof of concept study.

    PubMed

    Stanković, Kristina; Booth, Brian G; Danckaers, Femke; Burg, Fien; Vermaelen, Philippe; Duerinck, Saartje; Sijbers, Jan; Huysmans, Toon

    2018-01-01

    Foot morphology has received increasing attention from both biomechanics researches and footwear manufacturers. Usually, the morphology of the foot is quantified by 2D footprints. However, footprint quantification ignores the foot's vertical dimension and hence, does not allow accurate quantification of complex 3D foot shape. The shape variation of healthy 3D feet in a population of 31 adult women and 31 adult men who live in Belgium was studied using geometric morphometric methods. The effect of different factors such as sex, age, shoe size, frequency of sport activity, Body Mass Index (BMI), foot asymmetry, and foot loading on foot shape was investigated. Correlation between these factors and foot shape was examined using multivariate linear regression. The complex nature of a foot's 3D shape leads to high variability in healthy populations. After normalizing for scale, the major axes of variation in foot morphology are (in order of decreasing variance): arch height, combined ball width and inter-toe distance, global foot width, hallux bone orientation (valgus-varus), foot type (e.g. Egyptian, Greek), and midfoot width. These first six modes of variation capture 92.59% of the total shape variation. Higher BMI results in increased ankle width, Achilles tendon width, heel width and a thicker forefoot along the dorsoplantar axis. Age was found to be associated with heel width, Achilles tendon width, toe height and hallux orientation. A bigger shoe size was found to be associated with a narrow Achilles tendon, a hallux varus, a narrow heel, heel expansion along the posterior direction, and a lower arch compared to smaller shoe size. Sex was found to be associated with differences in ankle width, Achilles tendon width, and heel width. Frequency of sport activity was associated with Achilles tendon width and toe height. A detailed analysis of the 3D foot shape, allowed by geometric morphometrics, provides insights in foot variations in three dimensions that can not be obtained from 2D footprints. These insights could be applied in various scientific disciplines, including orthotics and shoe design.

  14. Controlling Particle Morphologies at Fluid Interfaces: Macro- and Micro- approaches

    NASA Astrophysics Data System (ADS)

    Beesabathuni, Shilpa Naidu

    The controlled generation of varying shaped particles is important for many applications: consumer goods, biomedical diagnostics, food processing, adsorbents and pharmaceuticals which can benefit from the availability of geometrically complex and chemically inhomogeneous particles. This thesis presents two approaches to spherical and non-spherical particle synthesis using macro and microfluidics. In the first approach, a droplet microfluidic technique is explored to fabricate spherical conducting polymer, polyaniline, particles with precise control over morphology and functionality. Microfluidics has recently emerged as an important alternate to the synthesis of complex particles. The conducting polymer, polyaniline, is widely used and known for its stability, high conductivity, and favorable redox properties. In this approach, monodisperse micron-sized polyaniline spherical particles were synthesized using two-phase droplet microfluidics from Aniline and Ammonium persulfate oxidative polymerization in an oil-based continuous phase. The morphology of the polymerized particles is porous in nature which can be used for encapsulation as well as controlled release applications. Encapsulation of an enzyme, glucose oxidase, was also performed using the technique to synthesize microspheres for glucose sensing. The polymer microspheres were characterized using SEM, UV-Vis and EDX to understand the relationship between their microstructure and stability. In the second approach, molten drop impact in a cooling aqueous medium to generate non-spherical particles was explored. Viscoelastic wax based materials are widely used in many applications and their performance and application depends on the particle morphology and size. The deformation of millimeter size molten wax drops as they impacted an immiscible liquid interface was investigated. Spherical molten wax drops impinged on a cooling water bath, then deformed and as a result of solidification were arrested into various shapes such as ellipsoids, mushrooms, spherulites and discs. The final morphology of the wax particles is governed by the interfacial, inertial, viscous and thermal effects, which can be studied over a range of Weber, Capillary, Reynolds and Stefan numbers. A simplified Stefan problem for a spherical drop was solved. The time required to initiate a phase transition at the interface of the molten wax and water after impact was estimated and correlated with the drop deformation history and final wax particle shape to develop a capability to predict the shape. While the microfluidic synthesis approach offers precise control over morphology and functionality, large particle throughput is a limitation. The drop impact in a liquid medium emulsion approach is limited to crosslinking or heat sensitive materials but can be extended to large scale production for industrial applications. Both approaches are simple, robust and cost effective making them viable and attractive solutions for complex particle synthesis. The choice of the approach is dependent on considerations such as particle material, size, shape, throughput and end application.

  15. Disentangling the phylogenetic and ecological components of spider phenotypic variation.

    PubMed

    Gonçalves-Souza, Thiago; Diniz-Filho, José Alexandre Felizola; Romero, Gustavo Quevedo

    2014-01-01

    An understanding of how the degree of phylogenetic relatedness influences the ecological similarity among species is crucial to inferring the mechanisms governing the assembly of communities. We evaluated the relative importance of spider phylogenetic relationships and ecological niche (plant morphological variables) to the variation in spider body size and shape by comparing spiders at different scales: (i) between bromeliads and dicot plants (i.e., habitat scale) and (ii) among bromeliads with distinct architectural features (i.e., microhabitat scale). We partitioned the interspecific variation in body size and shape into phylogenetic (that express trait values as expected by phylogenetic relationships among species) and ecological components (that express trait values independent of phylogenetic relationships). At the habitat scale, bromeliad spiders were larger and flatter than spiders associated with the surrounding dicots. At this scale, plant morphology sorted out close related spiders. Our results showed that spider flatness is phylogenetically clustered at the habitat scale, whereas it is phylogenetically overdispersed at the microhabitat scale, although phylogenic signal is present in both scales. Taken together, these results suggest that whereas at the habitat scale selective colonization affect spider body size and shape, at fine scales both selective colonization and adaptive evolution determine spider body shape. By partitioning the phylogenetic and ecological components of phenotypic variation, we were able to disentangle the evolutionary history of distinct spider traits and show that plant architecture plays a role in the evolution of spider body size and shape. We also discussed the relevance in considering multiple scales when studying phylogenetic community structure.

  16. Disentangling the Phylogenetic and Ecological Components of Spider Phenotypic Variation

    PubMed Central

    Gonçalves-Souza, Thiago; Diniz-Filho, José Alexandre Felizola; Romero, Gustavo Quevedo

    2014-01-01

    An understanding of how the degree of phylogenetic relatedness influences the ecological similarity among species is crucial to inferring the mechanisms governing the assembly of communities. We evaluated the relative importance of spider phylogenetic relationships and ecological niche (plant morphological variables) to the variation in spider body size and shape by comparing spiders at different scales: (i) between bromeliads and dicot plants (i.e., habitat scale) and (ii) among bromeliads with distinct architectural features (i.e., microhabitat scale). We partitioned the interspecific variation in body size and shape into phylogenetic (that express trait values as expected by phylogenetic relationships among species) and ecological components (that express trait values independent of phylogenetic relationships). At the habitat scale, bromeliad spiders were larger and flatter than spiders associated with the surrounding dicots. At this scale, plant morphology sorted out close related spiders. Our results showed that spider flatness is phylogenetically clustered at the habitat scale, whereas it is phylogenetically overdispersed at the microhabitat scale, although phylogenic signal is present in both scales. Taken together, these results suggest that whereas at the habitat scale selective colonization affect spider body size and shape, at fine scales both selective colonization and adaptive evolution determine spider body shape. By partitioning the phylogenetic and ecological components of phenotypic variation, we were able to disentangle the evolutionary history of distinct spider traits and show that plant architecture plays a role in the evolution of spider body size and shape. We also discussed the relevance in considering multiple scales when studying phylogenetic community structure. PMID:24651264

  17. Flow and fouling in membrane filters: Effects of membrane morphology

    NASA Astrophysics Data System (ADS)

    Sanaei, Pejman; Cummings, Linda J.

    2015-11-01

    Membrane filters are widely-used in microfiltration applications. Many types of filter membranes are produced commercially, for different filtration applications, but broadly speaking the requirements are to achieve fine control of separation, with low power consumption. The answer to this problem might seem obvious: select the membrane with the largest pore size and void fraction consistent with the separation requirements. However, membrane fouling (an inevitable consequence of successful filtration) is a complicated process, which depends on many parameters other than membrane pore size and void fraction; and which itself greatly affects the filtration process and membrane functionality. In this work we formulate mathematical models that can (i) account for the membrane internal morphology (internal structure, pore size & shape, etc.); (ii) fouling of membranes with specific morphology; and (iii) make some predictions as to what type of membrane morphology might offer optimum filtration performance.

  18. Identification of gender in yellow perch by external morphology: validation in four geographic strains and effects of estradiol

    USDA-ARS?s Scientific Manuscript database

    External morphological criteria that enable the rapid determination of gender have been developed for yellow perch (Perca flavescens). Criteria are based upon 1) shape of the urogenital papilla (UGP), 2) relative size of the UGP to the anal (AN) opening, and 3) coloration of the UGP. In females, t...

  19. Making quantitative morphological variation from basic developmental processes: where are we? The case of the Drosophila wing

    PubMed Central

    Alexis, Matamoro-Vidal; Isaac, Salazar-Ciudad; David, Houle

    2015-01-01

    One of the aims of evolutionary developmental biology is to discover the developmental origins of morphological variation. The discipline has mainly focused on qualitative morphological differences (e.g., presence or absence of a structure) between species. Studies addressing subtle, quantitative variation are less common. The Drosophila wing is a model for the study of development and evolution, making it suitable to investigate the developmental mechanisms underlying the subtle quantitative morphological variation observed in nature. Previous reviews have focused on the processes involved in wing differentiation, patterning and growth. Here, we investigate what is known about how the wing achieves its final shape, and what variation in development is capable of generating the variation in wing shape observed in nature. Three major developmental stages need to be considered: larval development, pupariation, and pupal development. The major cellular processes involved in the determination of tissue size and shape are cell proliferation, cell death, oriented cell division and oriented cell intercalation. We review how variation in temporal and spatial distribution of growth and transcription factors affects these cellular mechanisms, which in turn affects wing shape. We then discuss which aspects of the wing morphological variation are predictable on the basis of these mechanisms. PMID:25619644

  20. The importance of nanoparticle shape in cancer drug delivery.

    PubMed

    Truong, Nghia P; Whittaker, Michael R; Mak, Catherine W; Davis, Thomas P

    2015-01-01

    Nanoparticles have been successfully used for cancer drug delivery since 1995. In the design of commercial nanoparticles, size and surface characteristics have been exploited to achieve efficacious delivery. However, the design of optimized drug delivery platforms for efficient delivery to disease sites with minimal off-target effects remains a major research goal. One crucial element of nanoparticle design influencing both pharmacokinetics and cell uptake is nanoparticle morphology (both size and shape). In this succinct review, the authors collate the recent literature to assess the current state of understanding of the influence of nanoparticle shape on the effectiveness of drug delivery with a special emphasis on cancer therapy. This review draws on studies that have focused on the role of nonspherical nanoparticles used for cancer drug delivery. In particular, the authors summarize the influence of nanoparticle shape on biocirculation, biodistribution, cellular uptake and overall drug efficacy. By comparing spherical and nonspherical nanoparticles, they establish some general design principles to serve as guidelines for developing the next generation of nanocarriers for drug delivery. Pioneering studies on nanoparticles show that nonspherical shapes show great promise as cancer drug delivery vectors. Filamentous or worm-like micelles together with other rare morphologies such as needles or disks may become the norm for next-generation drug carriers, though at present, traditional spherical micelles remain the dominant shape of nanocarriers described in the literature due to synthesis and testing difficulties. The few reports that do exist describing nonspherical nanoparticles show a number of favorable properties that should encourage more efforts to develop facile and versatile nanoparticle synthesis methodologies with the flexibility to create different shapes, tunable sizes and adaptable surface chemistries. In addition, the authors note that there is a current lack of understanding into the factors governing (and optimizing) the inter-relationships of size, surface characteristics and shapes of many nanoparticles proposed for use in cancer therapy.

  1. A Three-Dimensional Analysis of Morphological Evolution and Locomotor Performance of the Carnivoran Forelimb

    PubMed Central

    Martín-Serra, Alberto; Figueirido, Borja; Palmqvist, Paul

    2014-01-01

    In this study, three-dimensional landmark-based methods of geometric morphometrics are used for estimating the influence of phylogeny, allometry and locomotor performance on forelimb shape in living and extinct carnivorans (Mammalia, Carnivora). The main objective is to investigate morphological convergences towards similar locomotor strategies in the shape of the major forelimb bones. Results indicate that both size and phylogeny have strong effects on the anatomy of all forelimb bones. In contrast, bone shape does not correlate in the living taxa with maximum running speed or daily movement distance, two proxies closely related to locomotor performance. A phylomorphospace approach showed that shape variation in forelimb bones mainly relates to changes in bone robustness. This indicates the presence of biomechanical constraints resulting from opposite demands for energetic efficiency in locomotion –which would require a slender forelimb– and resistance to stress –which would be satisfied by a robust forelimb–. Thus, we interpret that the need of maintaining a trade-off between both functional demands would limit shape variability in forelimb bones. Given that different situations can lead to one or another morphological solution, depending on the specific ecology of taxa, the evolution of forelimb morphology represents a remarkable “one-to-many mapping” case between anatomy and ecology. PMID:24454891

  2. Ontogenetic shape changes and sexual dimorphism in Aegla marginata Bond-Buckup and Buckup, 1994.

    PubMed

    Adam, Carolina L; Marochi, Murilo Z; Masunari, Setuko

    2018-05-14

    A study on relative growth, sexual dimorphism and ontogenetic trajectory was carried out in a population of the aeglidAegla marginata coming from Barrinha River, Iguape River Basin, Tunas do Paraná, Paraná State, Brazil. The size the of morphological sexual maturity was estimated for males and females. The analysis of sexual dimorphism and ontogenetic trajectory were performed using geometric morphometric technique. Males reach maturity with 10.58 mm of carapace length (CL) and females with 10.38 mm CL. Sexual size dimorphism was only visible among adults, with males reaching larger sizes. This is probably related to the reproductive strategy of males. However, sexual shape dimorphism was found for both juveniles and adults: the posterior region of the carapace was wider in females. As the contrast of this feature was stronger in adults, it can be considered that large abdomen is advantageous for egg incubation. The allometric trajectories of juveniles presented similar directions, becoming divergent during the adult phase. The shape variation inA. marginataoccurred gradually throughout its development, with no abrupt transformation upon reaching sexual maturity. The reproductive adaptation is the main reason for the morphological variation within populations ofA. marginata.

  3. Effect of amylose, particle size & morphology on the functionality of starches of traditional rice cultivars.

    PubMed

    Bhat, Farhan Mohiuddin; Riar, Charanjit Singh

    2016-11-01

    The research was carried out to investigate the effect of starch powder particle size, morphology, amylose content and varietal effect on physicochemical, X-ray diffraction pattern, thermal and pasting characteristics. The results indicated that starches isolated from seven traditional rice cultivars of temperate region of India have possessed higher yield (82.47-86.83%) with lower degree of granule damage and higher level of starch crystallinity (36.55-39.15%). The water and oil binding capacities were observed to correlate positively with amylose content. The bulk density and color parameters of starches were found to have linked with starch powder particle size coupled with arrangement and morphology of the starch granules. The rice cultivars having smaller starch powder particle size indicated lowest degree of crystallinity. Morphological studies revealed that the starches with tightly packed granules had greater mean granular width, while granules with openly spaced granular morphology depicted the higher values for mean granular length. The peak height index (PHI) among different starches ranged from 1.01 to 2.57 whereas the gelatinization range varied from 10.66 to 10.88. Concluding, the differences in distributional pattern of starch granule size and shape and powder particle size indicated a significant effect on the functional properties of starch. Copyright © 2016 Elsevier B.V. All rights reserved.

  4. Characterizing string-of-pearls colloidal silica by multidetector hydrodynamic chromatography and comparison to multidetector size-exclusion chromatography, off-line multiangle static light scattering, and transmission electron microscopy.

    PubMed

    Brewer, Amandaa K; Striegel, André M

    2011-04-15

    The string-of-pearls-type morphology is ubiquitous, manifesting itself variously in proteins, vesicles, bacteria, synthetic polymers, and biopolymers. Characterizing the size and shape of analytes with such morphology, however, presents a challenge, due chiefly to the ease with which the "strings" can be broken during chromatographic analysis or to the paucity of information obtained from the benchmark microscopy and off-line light scattering methods. Here, we address this challenge with multidetector hydrodynamic chromatography (HDC), which has the ability to determine, simultaneously, the size, shape, and compactness and their distributions of string-of-pearls samples. We present the quadruple-detector HDC analysis of colloidal string-of-pearls silica, employing static multiangle and quasielastic light scattering, differential viscometry, and differential refractometry as detection methods. The multidetector approach shows a sample that is broadly polydisperse in both molar mass and size, with strings ranging from two to five particles, but which also contains a high concentration of single, unattached "pearls". Synergistic combination of the various size parameters obtained from the multiplicity of detectors employed shows that the strings with higher degrees of polymerization have a shape similar to the theory-predicted shape of a Gaussian random coil chain of nonoverlapping beads, while the strings with lower degrees of polymerization have a prolate ellipsoidal shape. The HDC technique is contrasted experimentally with multidetector size-exclusion chromatography, where, even under extremely gentle conditions, the strings still degraded during analysis. Such degradation is shown to be absent in HDC, as evidenced by the fact that the molar mass and radius of gyration obtained by HDC with multiangle static light scattering detection (HDC/MALS) compare quite favorably to those determined by off-line MALS analysis under otherwise identical conditions. The multidetector HDC results were also comparable to those obtained by transmission electron microscopy (TEM). Unlike off-line MALS or TEM, however, multidetector HDC is able to provide complete particle analysis based on the molar mass, size, shape, and compactness and their distributions for the entire sample population in less than 20 min. © 2011 American Chemical Society

  5. Mathematical morphology-based shape feature analysis for Chinese character recognition systems

    NASA Astrophysics Data System (ADS)

    Pai, Tun-Wen; Shyu, Keh-Hwa; Chen, Ling-Fan; Tai, Gwo-Chin

    1995-04-01

    This paper proposes an efficient technique of shape feature extraction based on the application of mathematical morphology theory. A new shape complexity index for preclassification of machine printed Chinese Character Recognition (CCR) is also proposed. For characters represented in different fonts/sizes or in a low resolution environment, a more stable local feature such as shape structure is preferred for character recognition. Morphological valley extraction filters are applied to extract the protrusive strokes from four sides of an input Chinese character. The number of extracted local strokes reflects the shape complexity of each side. These shape features of characters are encoded as corresponding shape complexity indices. Based on the shape complexity index, data base is able to be classified into 16 groups prior to recognition procedures. The performance of associating with shape feature analysis reclaims several characters from misrecognized character sets and results in an average of 3.3% improvement of recognition rate from an existing recognition system. In addition to enhance the recognition performance, the extracted stroke information can be further analyzed and classified its own stroke type. Therefore, the combination of extracted strokes from each side provides a means for data base clustering based on radical or subword components. It is one of the best solutions for recognizing high complexity characters such as Chinese characters which are divided into more than 200 different categories and consist more than 13,000 characters.

  6. Variation in the shape and mechanical performance of the lower jaws in ceratopsid dinosaurs (Ornithischia, Ceratopsia).

    PubMed

    Maiorino, Leonardo; Farke, Andrew A; Kotsakis, Tassos; Teresi, Luciano; Piras, Paolo

    2015-11-01

    Ceratopsidae represents a group of quadrupedal herbivorous dinosaurs that inhabited western North America and eastern Asia during the Late Cretaceous. Although horns and frills of the cranium are highly variable across species, the lower jaw historically has been considered to be relatively conservative in morphology. Here, the lower jaws from 58 specimens representing 21 ceratopsoid taxa were sampled, using geometric morphometrics and 2D finite element analysis (FEA) to explore differences in morphology and mechanical performance across Ceratopsoidea (the clade including Ceratopsidae, Turanoceratops and Zuniceratops). Principal component analyses and non-parametric permuted manovas highlight Triceratopsini as a morphologically distinct clade within the sample. A relatively robust and elongate dentary, a larger and more elongated coronoid process, and a small and dorso-ventrally compressed angular characterize this clade, as well as the absolutely larger size. By contrast, non-triceratopsin chasmosaurines, Centrosaurini and Pachyrhinosaurini have similar morphologies to each other. Zuniceratops and Avaceratops are distinct from other taxa. No differences in size between Pachyrhinosaurini and Centrosaurini are recovered using non-parametric permuted anovas. Structural performance, as evaluated using a 2D FEA, is similar across all groups as measured by overall stress, with the exception of Triceratopsini. Shape, size and stress are phylogenetically constrained. A longer dentary as well as a long coronoid process result in a lower jaw that is reconstructed as relatively much more stressed in triceratopsins. © 2015 Anatomical Society.

  7. Genetic backgrounds and redox conditions influence morphological characteristics and cell differentiation of osteoclasts in mice.

    PubMed

    Narahara, Shun; Matsushima, Haruna; Sakai, Eiko; Fukuma, Yutaka; Nishishita, Kazuhisa; Okamoto, Kuniaki; Tsukuba, Takayuki

    2012-04-01

    Osteoclasts (OCLs) are multinucleated giant cells and are formed by the fusion of mononuclear progenitors of monocyte/macrophage lineage. It is known that macrophages derived from different genetic backgrounds exhibit quite distinct characteristics of immune responses. However, it is unknown whether OCLs from different genetic backgrounds show distinct characteristics. In this study, we showed that bone-marrow macrophages (BMMs) derived from C57BL/6, BALB/c and ddY mice exhibited considerably distinct morphological characteristics and cell differentiation into OCLs. The differentiation of BMMs into OCLs was comparatively quicker in the C57BL/6 and ddY mice, while that of BALB/c mice was rather slow. Morphologically, ddY OCLs showed a giant cell with a round shape, C57BL/6 OCLs were of a moderate size with many protrusions and BALB/c OCLs had the smallest size with fewer nuclei. The intracellular signaling of differentiation and expression levels of marker proteins of OCLs were different in the respective strains. Treatment of BMMs from the three different strains with the reducing agent N-acetylcysteine (NAC) or with the oxidation agent hydrogen peroxide (H(2)O(2)) induced changes in the shape and sizes of the cells and caused distinct patterns of cell differentiation and survival. Thus, genetic backgrounds and redox conditions regulate the morphological characteristics and cell differentiation of OCLs.

  8. Human cranial anatomy and the differential preservation of population history and climate signatures.

    PubMed

    Harvati, Katerina; Weaver, Timothy D

    2006-12-01

    Cranial morphology is widely used to reconstruct evolutionary relationships, but its reliability in reflecting phylogeny and population history has been questioned. Some cranial regions, particularly the face and neurocranium, are believed to be influenced by the environment and prone to convergence. Others, such as the temporal bone, are thought to reflect more accurately phylogenetic relationships. Direct testing of these hypotheses was not possible until the advent of large genetic data sets. The few relevant studies in human populations have had intriguing but possibly conflicting results, probably partly due to methodological differences and to the small numbers of populations used. Here we use three-dimensional (3D) geometric morphometrics methods to test explicitly the ability of cranial shape, size, and relative position/orientation of cranial regions to track population history and climate. Morphological distances among 13 recent human populations were calculated from four 3D landmark data sets, respectively reflecting facial, neurocranial, and temporal bone shape; shape and relative position; overall cranial shape; and centroid sizes. These distances were compared to neutral genetic and climatic distances among the same, or closely matched, populations. Results indicate that neurocranial and temporal bone shape track neutral genetic distances, while facial shape reflects climate; centroid size shows a weak association with climatic variables; and relative position/orientation of cranial regions does not appear correlated with any of these factors. Because different cranial regions preserve population history and climate signatures differentially, caution is suggested when using cranial anatomy for phylogenetic reconstruction. Copyright (c) 2006 Wiley-Liss, Inc.

  9. Providing a harmonious smile with laminate veneers for a patient with peg-shaped lateral incisors

    PubMed Central

    Alberton, Simone Beatriz; Alberton, Victória; de Carvalho, Rodrigo Varella

    2017-01-01

    The most common discrepancy concerning tooth size is the presence of peg-shaped lateral incisors, which can cause teeth with reduced size, anterior diastema, and consequently an unpleasant smile. The development of the adhesive dentistry and ceramic veneers allowed all ceramic-bonded restorations to become an esthetic and functional approach to reestablish the smile harmony. The purpose of this clinical report is to describe a conservative treatment approach to recover an esthetic disharmony caused by bilateral peg-shaped lateral incisors. A 30-year-old female patient was seeking treatment to solve an esthetic disharmony of her smile. Lithium disilicate veneers were planned and build-up with the help of diagnostic models, wax-up, mock-up, and silicone guides, to restore the morphology, size, function, and esthetic of upper lateral incisors and smile. The lithium disilicate veneers were able to provide the harmonization of the patient's smile, recovering the teeth size, shape, and anatomic characteristics. PMID:29279628

  10. Providing a harmonious smile with laminate veneers for a patient with peg-shaped lateral incisors.

    PubMed

    Alberton, Simone Beatriz; Alberton, Victória; de Carvalho, Rodrigo Varella

    2017-01-01

    The most common discrepancy concerning tooth size is the presence of peg-shaped lateral incisors, which can cause teeth with reduced size, anterior diastema, and consequently an unpleasant smile. The development of the adhesive dentistry and ceramic veneers allowed all ceramic-bonded restorations to become an esthetic and functional approach to reestablish the smile harmony. The purpose of this clinical report is to describe a conservative treatment approach to recover an esthetic disharmony caused by bilateral peg-shaped lateral incisors. A 30-year-old female patient was seeking treatment to solve an esthetic disharmony of her smile. Lithium disilicate veneers were planned and build-up with the help of diagnostic models, wax-up, mock-up, and silicone guides, to restore the morphology, size, function, and esthetic of upper lateral incisors and smile. The lithium disilicate veneers were able to provide the harmonization of the patient's smile, recovering the teeth size, shape, and anatomic characteristics.

  11. The effects of relative food item size on optimal tooth cusp sharpness during brittle food item processing

    PubMed Central

    Berthaume, Michael A.; Dumont, Elizabeth R.; Godfrey, Laurie R.; Grosse, Ian R.

    2014-01-01

    Teeth are often assumed to be optimal for their function, which allows researchers to derive dietary signatures from tooth shape. Most tooth shape analyses normalize for tooth size, potentially masking the relationship between relative food item size and tooth shape. Here, we model how relative food item size may affect optimal tooth cusp radius of curvature (RoC) during the fracture of brittle food items using a parametric finite-element (FE) model of a four-cusped molar. Morphospaces were created for four different food item sizes by altering cusp RoCs to determine whether optimal tooth shape changed as food item size changed. The morphospaces were also used to investigate whether variation in efficiency metrics (i.e. stresses, energy and optimality) changed as food item size changed. We found that optimal tooth shape changed as food item size changed, but that all optimal morphologies were similar, with one dull cusp that promoted high stresses in the food item and three cusps that acted to stabilize the food item. There were also positive relationships between food item size and the coefficients of variation for stresses in food item and optimality, and negative relationships between food item size and the coefficients of variation for stresses in the enamel and strain energy absorbed by the food item. These results suggest that relative food item size may play a role in selecting for optimal tooth shape, and the magnitude of these selective forces may change depending on food item size and which efficiency metric is being selected. PMID:25320068

  12. Growth and characterization of ZnO multipods on functional surfaces with different sizes and shapes of Ag particles

    NASA Astrophysics Data System (ADS)

    A, Kamalianfar; S, A. Halim; Mahmoud Godarz, Naseri; M, Navasery; Fasih, Ud Din; J, A. M. Zahedi; Kasra, Behzad; K, P. Lim; A Lavari, Monghadam; S, K. Chen

    2013-08-01

    Three-dimensional ZnO multipods are successfully synthesized on functional substrates using the vapor transport method in a quartz tube. The functional surfaces, which include two different distributions of Ag nanoparticles and a layer of commercial Ag nanowires, are coated onto silicon substrates before the growth of ZnO nanostructures. The structures and morphologies of the ZnO/Ag heterostructures are investigated using X-ray diffraction and field emission scanning electron microscopy. The sizes and shapes of the Ag particles affect the growth rates and initial nucleations of the ZnO structures, resulting in different numbers and shapes of multipods. They also influence the orientation and growth quality of the rods. The optical properties are studied by photoluminescence, UV-vis, and Raman spectroscopy. The results indicate that the surface plasmon resonance strongly depends on the sizes and shapes of the Ag particles.

  13. Bio-inspired routes for synthesizing efficient nanoscale platinum electrocatalysts

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Cha, Jennifer N.; Wang, Joseph

    2014-08-31

    The overall objective of the proposed research is to use fundamental advances in bionanotechnology to design powerful platinum nanocrystal electrocatalysts for fuel cell applications. The new economically-viable, environmentally-friendly, bottom-up biochemical synthetic strategy will produce platinum nanocrystals with tailored size, shape and crystal orientation, hence leading to a maximum electrochemical reactivity. There are five specific aims to the proposed bio-inspired strategy for synthesizing efficient electrocatalytic platinum nanocrystals: (1) isolate peptides that both selectively bind particular crystal faces of platinum and promote the nucleation and growth of particular nanocrystal morphologies, (2) pattern nanoscale 2-dimensional arrays of platinum nucleating peptides from DNA scaffolds,more » (3) investigate the combined use of substrate patterned peptides and soluble peptides on nanocrystal morphology and growth (4) synthesize platinum crystals on planar and large-area carbon electrode supports, and (5) perform detailed characterization of the electrocatalytic behavior as a function of catalyst size, shape and morphology. Project Description and Impact: This bio-inspired collaborative research effort will address key challenges in designing powerful electrocatalysts for fuel cell applications by employing nucleic acid scaffolds in combination with peptides to perform specific, environmentally-friendly, simultaneous bottom-up biochemical synthesis and patterned assembly of highly uniform and efficient platinum nanocrystal catalysts. Bulk synthesis of nanoparticles usually produces a range of sizes, accessible catalytic sites, crystal morphologies, and orientations, all of which lead to inconsistent catalytic activities. In contrast, biological systems routinely demonstrate exquisite control over inorganic syntheses at neutral pH and ambient temperature and pressures. Because the orientation and arrangement of the templating biomolecules can be precisely controlled, the nanocrystals boast a defined shape, morphology, orientation and size and are synthesized at benign reaction conditions. Adapting the methods of biomineralization towards the synthesis of platinum nanocrystals will allow effective control at a molecular level of the synthesis of highly active metal electrocatalysts, with readily tailored properties, through tuning of the biochemical inputs. The proposed research will incorporate many facets of biomineralization by: (1) isolating peptides that selectively bind particular crystal faces of platinum (2) isolating peptides that promote the nucleation and growth of particular nanocrystal morphologies (3) using two-dimensional DNA scaffolds to control the spatial orientation and density of the platinum nucleating peptides, and (4) combining bio-templating and soluble peptides to control crystal nucleation, orientation, and morphology. The resulting platinum nanocrystals will be evaluated for their electrocatalytic behavior (on common carbon supports) to determine their optimal size, morphology and crystal structure. We expect that such rational biochemical design will lead to highly uniform and efficient platinum nanocrystal catalysts for fuel cell applications.« less

  14. Evaluation of Morphological Change and Aggregation Process of Ice Crystals in Frozen Food by Using Fractal Analysis

    NASA Astrophysics Data System (ADS)

    Koshiro, Yoko; Watanabe, Manabu; Takai, Rikuo; Hagiwara, Tomoaki; Suzuki, Toru

    Size and shape of ice crystals in frozen food materials are very important because they affect not only quality of foods but also the viability of industrial processing such as freeze-drying of concentration. In this study, 30%wt sucrose solution is used as test samples. For examining the effect of stabilizerspectine and xantan gum is added to the sucrose solution. They are frozen on the cold stage of microscope to be observed their growing ice crystals under the circumstance of -10°C. Their size and shape are measured and quantitatively evaluated by applying fractal analysis. lce crystal of complicated shape has large fractal dimension, and vice versa. It successflly categorized the ice crystals into two groups; one is a group of large size and complicated shape, and the other is a group of small size and plain shape. The critical crystal size between the two groups is found to become larger with increasing holding time. It suggests a phenomenological model for metamorphoses process of ice crystals. Further, it is indicated that xantan gum is able to suppress the smoothing of ice crystals.

  15. Porous inorganic-organic shape memory polymers.

    PubMed

    Zhang, Dawei; Burkes, William L; Schoener, Cody A; Grunlan, Melissa A

    2012-06-21

    Thermoresponsive shape memory polymers (SMPs) are a type of stimuli-sensitive materials that switch from a temporary shape back to their permanent shape upon exposure to heat. While the majority of SMPs have been fabricated in the solid form, porous SMP foams exhibit distinct properties and are better suited for certain applications, including some in the biomedical field. Like solid SMPs, SMP foams have been restricted to a limited group of organic polymer systems. In this study, we prepared inorganic-organic SMP foams based on the photochemical cure of a macromer comprised of inorganic polydimethylsiloxane (PDMS) segments and organic poly(ε-caprolactone) (PCL) segments, diacrylated PCL(40)-block-PDMS(37)-block-PCL(40). To achieve tunable pore size with high interconnectivity, the SMP foams were prepared via a refined solvent-casting/particulate-leaching (SCPL) method. By varying design parameters such as degree of salt fusion, macromer concentration in the solvent and salt particle size, the SMP foams with excellent shape memory behavior and tunable pore size, pore morphology, and modulus were obtained.

  16. Early bursts of body size and shape evolution are rare in comparative data.

    PubMed

    Harmon, Luke J; Losos, Jonathan B; Jonathan Davies, T; Gillespie, Rosemary G; Gittleman, John L; Bryan Jennings, W; Kozak, Kenneth H; McPeek, Mark A; Moreno-Roark, Franck; Near, Thomas J; Purvis, Andy; Ricklefs, Robert E; Schluter, Dolph; Schulte Ii, James A; Seehausen, Ole; Sidlauskas, Brian L; Torres-Carvajal, Omar; Weir, Jason T; Mooers, Arne Ø

    2010-08-01

    George Gaylord Simpson famously postulated that much of life's diversity originated as adaptive radiations-more or less simultaneous divergences of numerous lines from a single ancestral adaptive type. However, identifying adaptive radiations has proven difficult due to a lack of broad-scale comparative datasets. Here, we use phylogenetic comparative data on body size and shape in a diversity of animal clades to test a key model of adaptive radiation, in which initially rapid morphological evolution is followed by relative stasis. We compared the fit of this model to both single selective peak and random walk models. We found little support for the early-burst model of adaptive radiation, whereas both other models, particularly that of selective peaks, were commonly supported. In addition, we found that the net rate of morphological evolution varied inversely with clade age. The youngest clades appear to evolve most rapidly because long-term change typically does not attain the amount of divergence predicted from rates measured over short time scales. Across our entire analysis, the dominant pattern was one of constraints shaping evolution continually through time rather than rapid evolution followed by stasis. We suggest that the classical model of adaptive radiation, where morphological evolution is initially rapid and slows through time, may be rare in comparative data.

  17. Variations in interior morphology of 15-20 km lunar craters - Implications for a major subsurface discontinuity

    NASA Technical Reports Server (NTRS)

    De Hon, R. A.

    1980-01-01

    Craters vary in morphology as a function of crater diameter, age, and mode of origin. This study concentrates on the morphology of young lunar impact craters within a limited size range. Elimination of morphologic variations generally attributed to crater size or age leaves a small population which should nearly reflect the varying properties of the lunar substrate. The sample consists of 17 craters 15-20 km in diameter with both simple and complex morphologies. While depth/diameter ratios do not obviously differ between mare and highland subsets, apparent depth, rim height, and profile data do differ distinctly. Highland craters tend to be deep, simple, and bowl-shaped. Mare craters tend to be shallow and flat-floored. Rim heights of complex mare craters are typically greater than those of simple craters. Differences of highland and mare crater morphologies are attributed to variations in the thickness of the lunar megaregolith. Highland craters in this size range do not penetrate the megaregolith. The depth and morphology of complex craters are controlled by the discontinuity at the transition from highly brecciated megaregolith to more coherent crystalline material of the upper crust.

  18. Mapping genetic variants for cranial vault shape in humans.

    PubMed

    Roosenboom, Jasmien; Lee, Myoung Keun; Hecht, Jacqueline T; Heike, Carrie L; Wehby, George L; Christensen, Kaare; Feingold, Eleanor; Marazita, Mary L; Maga, A Murat; Shaffer, John R; Weinberg, Seth M

    2018-01-01

    The shape of the cranial vault, a region comprising interlocking flat bones surrounding the cerebral cortex, varies considerably in humans. Strongly influenced by brain size and shape, cranial vault morphology has both clinical and evolutionary relevance. However, little is known about the genetic basis of normal vault shape in humans. We performed a genome-wide association study (GWAS) on three vault measures (maximum cranial width [MCW], maximum cranial length [MCL], and cephalic index [CI]) in a sample of 4419 healthy individuals of European ancestry. All measures were adjusted by sex, age, and body size, then tested for association with genetic variants spanning the genome. GWAS results for the two cohorts were combined via meta-analysis. Significant associations were observed at two loci: 15p11.2 (lead SNP rs2924767, p = 2.107 × 10-8) for MCW and 17q11.2 (lead SNP rs72841279, p = 5.29 × 10-9) for MCL. Additionally, 32 suggestive loci (p < 5x10-6) were observed. Several candidate genes were located in these loci, such as NLK, MEF2A, SOX9 and SOX11. Genome-wide linkage analysis of cranial vault shape in mice (N = 433) was performed to follow-up the associated candidate loci identified in the human GWAS. Two loci, 17q11.2 (c11.loc44 in mice) and 17q25.1 (c11.loc74 in mice), associated with cranial vault size in humans, were also linked with cranial vault size in mice (LOD scores: 3.37 and 3.79 respectively). These results provide further insight into genetic pathways and mechanisms underlying normal variation in human craniofacial morphology.

  19. Mapping genetic variants for cranial vault shape in humans

    PubMed Central

    Lee, Myoung Keun; Hecht, Jacqueline T.; Heike, Carrie L.; Wehby, George L.; Christensen, Kaare; Feingold, Eleanor; Marazita, Mary L.; Weinberg, Seth M.

    2018-01-01

    The shape of the cranial vault, a region comprising interlocking flat bones surrounding the cerebral cortex, varies considerably in humans. Strongly influenced by brain size and shape, cranial vault morphology has both clinical and evolutionary relevance. However, little is known about the genetic basis of normal vault shape in humans. We performed a genome-wide association study (GWAS) on three vault measures (maximum cranial width [MCW], maximum cranial length [MCL], and cephalic index [CI]) in a sample of 4419 healthy individuals of European ancestry. All measures were adjusted by sex, age, and body size, then tested for association with genetic variants spanning the genome. GWAS results for the two cohorts were combined via meta-analysis. Significant associations were observed at two loci: 15p11.2 (lead SNP rs2924767, p = 2.107 × 10−8) for MCW and 17q11.2 (lead SNP rs72841279, p = 5.29 × 10−9) for MCL. Additionally, 32 suggestive loci (p < 5x10-6) were observed. Several candidate genes were located in these loci, such as NLK, MEF2A, SOX9 and SOX11. Genome-wide linkage analysis of cranial vault shape in mice (N = 433) was performed to follow-up the associated candidate loci identified in the human GWAS. Two loci, 17q11.2 (c11.loc44 in mice) and 17q25.1 (c11.loc74 in mice), associated with cranial vault size in humans, were also linked with cranial vault size in mice (LOD scores: 3.37 and 3.79 respectively). These results provide further insight into genetic pathways and mechanisms underlying normal variation in human craniofacial morphology. PMID:29698431

  20. Usefulness of the Ice-Cream Cone Pattern in Computed Tomography for Prediction of Angiomyolipoma in Patients With a Small Renal Mass

    PubMed Central

    Kim, Kwang Ho; Yun, Bu Hyeon; Hwang, In Sang; Hwang, Eu Chang; Kang, Taek Won; Kwon, Dong Deuk; Park, Kwangsung; Kim, Jin Woong

    2013-01-01

    Purpose A morphologic contour method for assessing an exophytic renal mass as benign versus malignant on the basis of the shape of the interface with the renal parenchyma was recently developed. We investigated the usefulness of this morphologic contour method for predicting angiomyolipoma (AML) in patients who underwent partial nephrectomy for small renal masses (SRMs). Materials and Methods From January 2004 to March 2013, among 197 patients who underwent partial nephrectomy for suspicious renal cell carcinoma (RCC), the medical records of 153 patients with tumors (AML or RCC) ≤3 cm in diameter were retrospectively reviewed. Patient characteristics including age, gender, type of surgery, size and location of tumor, pathologic results, and specific findings of the imaging study ("ice-cream cone" shape) were compared between the AML and RCC groups. Results AML was diagnosed in 18 patients and RCC was diagnosed in 135 patients. Gender (p=0.001), tumor size (p=0.032), and presence of the ice-cream cone shape (p=0.001) showed statistically significant differences between the AML group and the RCC group. In the multivariate logistic regression analysis, female gender (odds ratio [OR], 5.20; 95% confidence interval [CI], 1.45 to 18.57; p=0.011), tumor size (OR, 0.34; 95% CI, 0.12 to 0.92; p=0.034), and presence of the ice-cream cone shape (OR, 18.12; 95% CI, 4.97 to 66.06; p=0.001) were predictors of AML. Conclusions This study confirmed a high incidence of AML in females. Also, the ice-cream cone shape and small tumor size were significant predictors of AML in SRMs. These finding could be beneficial for counseling patients with SRMs. PMID:23956824

  1. 3D-shape recognition and size measurement of irregular rough particles using multi-views interferometric out-of-focus imaging.

    PubMed

    Ouldarbi, L; Talbi, M; Coëtmellec, S; Lebrun, D; Gréhan, G; Perret, G; Brunel, M

    2016-11-10

    We realize simplified-tomography experiments on irregular rough particles using interferometric out-of-focus imaging. Using two angles of view, we determine the global 3D-shape, the dimensions, and the 3D-orientation of irregular rough particles whose morphologies belong to families such as sticks, plates, and crosses.

  2. Habitat-Specific Morphological Variation among Threespine Sticklebacks (Gasterosteus aculeatus) within a Drainage Basin

    PubMed Central

    Webster, Mike M.; Atton, Nicola; Hart, Paul J. B.; Ward, Ashley J. W.

    2011-01-01

    Habitat-specific morphological variation, often corresponding to resource specialization, is well documented in freshwater fishes. In this study we used landmark based morphometric analyses to investigate morphological variation among threespine sticklebacks (Gasterosteus aculeatus L.) from four interconnected habitat types within a single lowland drainage basin in eastern England. These included the upper and lower reaches of the river, the estuary, a connected ditch network and a coastal salt marsh. We found significant habitat-specific differences in morphology, with three axes of variation describing differences in orbit diameter, body depth, caudal peduncle shape and pectoral fin positioning as well as variation in relative dorsal and pelvic spine size. Interestingly, the ditch system, an artificial and heavily managed habitat, is populated by sticklebacks with a characteristic morphology, suggesting that human management of habitats can in some circumstances lead to morphological variation among the animals that inhabit them. We discuss the mechanisms that conceivably underlie the observed morphological variation and the further work necessary to identify them. Finally, we consider the implications of habitat-specific body shape variation for the behavioural ecology of this ecologically generalist species. PMID:21698269

  3. The evolution of the platyrrhine talus: A comparative analysis of the phenetic affinities of the Miocene platyrrhines with their modern relatives.

    PubMed

    Püschel, Thomas A; Gladman, Justin T; Bobe, René; Sellers, William I

    2017-10-01

    Platyrrhines are a diverse group of primates that presently occupy a broad range of tropical-equatorial environments in the Americas. However, most of the fossil platyrrhine species of the early Miocene have been found at middle and high latitudes. Although the fossil record of New World monkeys has improved considerably over the past several years, it is still difficult to trace the origin of major modern clades. One of the most commonly preserved anatomical structures of early platyrrhines is the talus. This work provides an analysis of the phenetic affinities of extant platyrrhine tali and their Miocene counterparts through geometric morphometrics and a series of phylogenetic comparative analyses. Geometric morphometrics was used to quantify talar shape affinities, while locomotor mode percentages (LMPs) were used to test if talar shape is associated with locomotion. Comparative analyses were used to test if there was convergence in talar morphology, as well as different models that could explain the evolution of talar shape and size in platyrrhines. Body mass predictions for the fossil sample were also computed using the available articular surfaces. The results showed that most analyzed fossils exhibit a generalized morphology that is similar to some 'generalist' modern species. It was found that talar shape covaries with LMPs, thus allowing the inference of locomotion from talar morphology. The results further suggest that talar shape diversification can be explained by invoking a model of shifts in adaptive peak to three optima representing a phylogenetic hypothesis in which each platyrrhine family occupied a separate adaptive peak. The analyses indicate that platyrrhine talar centroid size diversification was characterized by an early differentiation related to a multidimensional niche model. Finally, the ancestral platyrrhine condition was reconstructed as a medium-sized, generalized, arboreal, quadruped. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  4. Morphological divergence in a continental adaptive radiation: South American ovenbirds of the genus Cinclodes

    USGS Publications Warehouse

    Rader, Jonathan A.; Dillon, Michael E.; Chesser, R. Terry; Sabat, Pablo; Martinez del Rio, Carlos

    2015-01-01

    Cinclodes is an ecologically diverse genus of South American passerine birds and represents a case of continental adaptive radiation along multiple axes. We investigated morphological diversification in Cinclodes using a comprehensive set of morphometric measurements of study skins. Principal component analysis identified 2 primary axes of morphological variation: one describing body size and a second capturing differences in wing-tip shape and toe length. Phylogenetic analyses of the first principal component suggest an early divergence ofCinclodes into 2 main clades characterized by large and small body sizes. We suggest that 2 morphological outliers within these main clades (C. antarcticus and C. palliatus) may be cases of island gigantism and that a third (C. patagonicus) may reflect ecological character displacement. Despite its ecological and physiological diversity, the genus Cinclodes does not appear to show morphological diversity beyond what is typical of other avian genera.

  5. Dynamic control of gold nanoparticle morphology in a microchannel flow reactor by glucose reduction in aqueous sodium hydroxide solution.

    PubMed

    Ishizaka, Takayuki; Ishigaki, Atsushi; Kawanami, Hajime; Suzuki, Akira; Suzuki, Toshishige M

    2012-02-01

    Continuous flow synthesis of gold nanoparticles was demonstrated using a microchannel reactor with glucose reduction in aqueous alkaline medium. Particle size, morphology, and visual/optical properties of the dispersion liquid were controlled dynamically by tuning of the rate of NaOH addition. Characteristic star-like nanoparticles formed spontaneously as a quasi-stable state, but they changed the morphology to round shape and showed spectral change over time. Copyright © 2011 Elsevier Inc. All rights reserved.

  6. Nucleation and growth mechanism for flame synthesis of MoO2 hollow microchannels with nanometer wall thickness.

    PubMed

    Merchan-Merchan, Wilson; Saveliev, Alexei V; Taylor, Aaron M

    2009-12-01

    The growth and morphological evolution of molybdenum-oxide microstructures formed in the high temperature environment of a counter-flow oxy-fuel flame using molybdenum probes is studied. Experiments conducted using various probe retention times show the sequence of the morphological changes. The morphological row begins with micron size objects exhibiting polygonal cubic shape, develops into elongated channels, changes to large structures with leaf-like shape, and ends in dendritic structures. Time of probe-flame interaction is found to be a governing parameter controlling the wide variety of morphological patterns; a molecular level growth mechanism is attributed to their development. This study reveals that the structures are grown in several consecutive stages: material "evaporation and transportation", "transformation", "nucleation", "initial growth", "intermediate growth", and "final growth". XRD analysis shows that the chemical compositions of all structures correspond to MoO(2).

  7. A morphogram for silica-witherite biomorphs and its application to microfossil identification in the early earth rock record.

    PubMed

    Rouillard, J; García-Ruiz, J-M; Gong, J; van Zuilen, M A

    2018-05-01

    Archean hydrothermal environments formed a likely site for the origin and early evolution of life. These are also the settings, however, were complex abiologic structures can form. Low-temperature serpentinization of ultramafic crust can generate alkaline, silica-saturated fluids in which carbonate-silica crystalline aggregates with life-like morphologies can self-assemble. These "biomorphs" could have adsorbed hydrocarbons from Fischer-Tropsch type synthesis processes, leading to metamorphosed structures that resemble carbonaceous microfossils. Although this abiogenic process has been extensively cited in the literature and has generated important controversy, so far only one specific biomorph type with a filamentous shape has been discussed for the interpretation of Archean microfossils. It is therefore critical to precisely determine the full distribution in morphology and size of these biomorphs, and to study the range of plausible geochemical conditions under which these microstructures can form. Here, a set of witherite-silica biomorph synthesis experiments in silica-saturated solutions is presented, for a range of pH values (from 9 to 11.5) and barium ion concentrations (from 0.6 to 40 mmol/L BaCl 2 ). Under these varying conditions, a wide range of life-like structures is found, from fractal dendrites to complex shapes with continuous curvature. The size, spatial concentration, and morphology of the biomorphs are strongly controlled by environmental parameters, among which pH is the most important. This potentially limits the diversity of environments in which the growth of biomorphs could have occurred on Early Earth. Given the variety of the observed biomorph morphologies, our results show that the morphology of an individual microstructure is a poor criterion for biogenicity. However, biomorphs may be distinguished from actual populations of cellular microfossils by their wide, unimodal size distribution. Biomorphs grown by diffusion in silica gel can be differentiated by their continuous gradient in size, spatial density, and morphology along the direction of diffusion. © 2018 The Authors. Geobiology Published by John Wiley & Sons Ltd.

  8. De novo comparative transcriptome analysis of genes involved in fruit morphology of pumpkin cultivars with extreme size difference and development of EST-SSR markers.

    PubMed

    Xanthopoulou, Aliki; Ganopoulos, Ioannis; Psomopoulos, Fotis; Manioudaki, Maria; Moysiadis, Theodoros; Kapazoglou, Aliki; Osathanunkul, Maslin; Michailidou, Sofia; Kalivas, Apostolos; Tsaftaris, Athanasios; Nianiou-Obeidat, Irini; Madesis, Panagiotis

    2017-07-30

    The genetic basis of fruit size and shape was investigated for the first time in Cucurbita species and genetic loci associated with fruit morphology have been identified. Although extensive genomic resources are available at present for tomato (Solanum lycopersicum), cucumber (Cucumis sativus), melon (Cucumis melo) and watermelon (Citrullus lanatus), genomic databases for Cucurbita species are limited. Recently, our group reported the generation of pumpkin (Cucurbita pepo) transcriptome databases from two contrasting cultivars with extreme fruit sizes. In the current study we used these databases to perform comparative transcriptome analysis in order to identify genes with potential roles in fruit morphology and fruit size. Differential Gene Expression (DGE) analysis between cv. 'Munchkin' (small-fruit) and cv. 'Big Moose' (large-fruit) revealed a variety of candidate genes associated with fruit morphology with significant differences in gene expression between the two cultivars. In addition, we have set the framework for generating EST-SSR markers, which discriminate different C. pepo cultivars and show transferability to related Cucurbitaceae species. The results of the present study will contribute to both further understanding the molecular mechanisms regulating fruit morphology and furthermore identifying the factors that determine fruit size. Moreover, they may lead to the development of molecular marker tools for selecting genotypes with desired morphological traits. Copyright © 2017. Published by Elsevier B.V.

  9. Cyclodextrin-assisted synthesis of tailored mesoporous silica nanoparticles

    PubMed Central

    2018-01-01

    Mesoporous silica nanoparticles (MSNs) have sparked considerable interest in drug/gene delivery, catalysis, adsorption, separation, sensing, antireflection coatings and bioimaging because of their tunable structural properties. The shape, size and pore structure of MSNs are greatly influenced by the type of additives used, e.g., solvent and pore-templating agent. Here, we studied the influence of cyclodextrin (CD) molecules on the formation of MSNs. The nanoparticles over 100 nm in diameter were synthesized by surfactant-templated, hydrolysis–polycondensation reactions in the presence of pristine CD (β-CD) or hydroxypropyl-functionalized CDs (HP-γ-CD and HP-β-CD). Depending on the formulation conditions, differently shaped MSNs, such as bean-like, spherical, ellipsoid, aggregate and faceted were generated. The morphology and size of MSNs varied with the CD-type used. Generally, spherical particles were obtained with β-CD, while a faceted morphology was observed for the particles synthesized using HP-CDs. The particle size could be tuned by adjusting the amount of CD used; increasing the CD concentration led to larger particles. MSNs synthesized in the presence of β-CD displayed a smaller particle size than those produced with HP-functional CDs. FTIR, TGA and solid-state 13C NMR demonstrated the adsorption of CDs on the particle surfaces. The proposed concept allows for the synthesis of silica nanoparticles with control over particle shape and size by adjusting the concentration of additives in a simple, one-pot reaction system for a wide range of applications. PMID:29527443

  10. Eye size and behaviour of day- and night-flying leafcutting ant alates

    Treesearch

    John C. Moser; John D. Reeve; José Maunício S. Bento; Terezinha M.C. Della Lucia; R. Scott Cameron; Natalie M. Heck

    2004-01-01

    The morphology of insect eyes often seems to be shaped by evolution to match their behaviour and lifestyle. Here the relationship between the nuptial flight behaviour of 10 Atta species (Hymenoptera: Fonnicidae) and the eye size of male and female alates, including the compound eyes, ommatidia facets, and ocelli were examined. These species can be...

  11. Complex pattern of variation in neurocranial ontogeny revealed by CT-scanning.

    PubMed

    Anzelmo, Marisol; Ventrice, Fernando; Kelmansky, Diana; Sardi, Marina

    2018-05-01

    The neurocranium of hominid species has been largely studied with reference to the midsagittal plane, with variations being attributed to brain evolution. By contrast, there is limited information on variation in non-midsagittal regions, which are the points of insertion of muscles and bony structures related to mastication. This work aims to analyze ontogenetic changes and sexual dimorphism (SD) in midsagittal and non-midsagittal neurocranial structures from a contemporary human sample comprising 138 computed tomography (CT) cranial images of individuals ranging from infants to adults. Morphology of the vault and the base was assessed by registering landmarks and semilandmarks, which were analyzed by geometric morphometrics, and the endocranial volume (EV). The results of regressions and Kruskal-Wallis test indicate that the major size and shape changes in both midsagittal and non-midsagittal regions occur during infancy and juvenility; shape changes are also associated with an increase in EV. The size of the midsagittal vault, the shape of the non-midsagittal vault and the size of the base show an extension of ontogenetic trajectories. Sexes show similar changes in shape but different changes in size. We conclude that brain growth appears to be an important factor influencing the morphology of the neurocranium, at least during infancy and childhood. Subsequent changes may be attributed to osteogenic activity and the differential growth of the brain lobes. Masticatory-related bony structures and muscles may not be strong enough factors to induce independent modifications in non-midsagittal structures. The small influence of the cranial muscles would explain why the human neurocranium is a quite integrated structure.

  12. Influences of powder granularity on crystallizing characteristics in mica-contained glass ceramic

    NASA Astrophysics Data System (ADS)

    Xu, L. N.; Kong, D. Y.; Tian, Q. B.; Lv, Z. J.

    2017-09-01

    A machinable mica-contained glass ceramic in the SiO2-Al2O3-MgO-F glassy system was prepared by ball milling and hot pressed sintering. Three kinds of powder sizes of base glass were chosen and the effects of the glass powder sizes on the crystallization were explored by x-ray diffraction and scanning electron microscopy techniques. The results indicate that mica crystal as a major phase and KFeSi2O6 and mullite as minor phases are crystallized. Applying pressure at 670°C has little influences on the types of crystal precipitated and the preferential growth of crystal. The powder sizes, however, have obvious effects on the morphology of precipitated mica crystals. In the glass sample with a powder size of d50=16.4 µm, the plate-shaped mica phase is precipitated. As the powder size decrease to 9.9 µm and 3.3 µm, however, the particle-shaped mica is formed instead of the plate-shaped crystals.

  13. Thermal diffusivity measurement for urchin-like gold nanofluids with different solvents, sizes and concentrations/shapes.

    PubMed

    López-Muñoz, Gerardo A; Balderas-López, José Abraham; Ortega-Lopez, Jaime; Pescador-Rojas, José A; Salazar, Jaime Santoyo

    2012-12-06

    The thermal properties of nanofluids are an especially interesting research topic because of the variety of potential applications, which range from bio-utilities to next-generation heat-transfer fluids. In this study, photopyroelectric calorimetry for measuring the thermal diffusivity of urchin-like colloidal gold nanofluids as a function of particle size, concentration and shape in water, ethanol and ethylene glycol is reported. Urchin-like gold nanoparticles were synthesised in the presence of hydroquinone through seed-mediated growth with homogeneous shape and size ranging from 55 to 115 nm. The optical response, size and morphology of these nanoparticles were characterised using UV-visible spectroscopy and transmission electron microscopy. The thermal diffusivity of these nanofluids decreased as the size of the nanoparticles increased, and the enhancement depended on the thermal diffusivity of the solvent. The opposite effect (increase in thermal diffusivity) was observed when the nanoparticle concentration was increased. These effects were more evident for urchin-like gold nanofluids than for the corresponding spherical gold nanofluids.

  14. Protein-free formation of bone-like apatite: New insights into the key role of carbonation

    PubMed Central

    Deymier, Alix C.; Nair, Arun K.; Depalle, Baptiste; Qin, Zhao; Arcot, Kashyap; Drouet, Christophe; Yoder, Claude H.; Buehler, Markus J.; Thomopoulos, Stavros; Genin, Guy M.; Pasteris, Jill D.

    2017-01-01

    The nanometer-sized plate-like morphology of bone mineral is necessary for proper bone mechanics and physiology. However, mechanisms regulating the morphology of these mineral nanocrystals remain unclear. The dominant hypothesis attributes the size and shape regulation to organic-mineral interactions. Here, we present data supporting the hypothesis that physicochemical effects of carbonate integration within the apatite lattice control the morphology, size, and mechanics of bioapatite mineral crystals. Carbonated apatites synthesized in the absence of organic molecules presented plate-like morphologies and nanoscale crystallite dimensions. Experimentally-determined crystallite size, lattice spacing, solubility and atomic order were modified by carbonate concentration. Molecular dynamics (MD) simulations and density functional theory (DFT) calculations predicted changes in surface energy and elastic moduli with carbonate concentration. Combining these results with a scaling law predicted the experimentally observed scaling of size and energetics with carbonate concentration. The experiments and models describe a clear mechanism by which crystal dimensions are controlled by carbonate substitution. Furthermore, the results demonstrate that carbonate substitution is sufficient to drive the formation of bone-like crystallites. This new understanding points to pathways for biomimetic synthesis of novel, nanostructured biomaterials. PMID:28279923

  15. North American sturgeon otolith morphology

    USGS Publications Warehouse

    Chalupnicki, Marc A.; Dittman, Dawn E.

    2016-01-01

    Accurate expedient species identification of deceased sturgeon (Acipenseridae) when external physical characteristic analysis is inconclusive has become a high priority due to the endangered or threatened status of sturgeon species around the world. Examination of otoliths has provided useful information to aid in population management, age and size-class analysis, understanding predator–prey interactions, and archeological research in other fish species. The relationship between otolith characteristics and sturgeon species has remained unknown. Therefore, we analyzed the shape of otoliths from the eight species of sturgeon found in North America to test the utility of otolith characteristic morphology in species identification. There were distinct differences in the size and shape of the otoliths between species of sturgeon with little shape variation among individuals of the same species. The relationship between otolith length axes was linear, and most of the variability was explained by a Log (axis + 1) transformation of the x and y axes (r2 = 0.8983) using the equation y = 0.73x + 0.0612. Images of otoliths from all eight North American species are presented to assist in the identification process.

  16. Synthesis of Mesoporous α-Fe2O3 Nanoparticles by Non-ionic Soft Template and Their Applications to Heavy Oil Upgrading

    NASA Astrophysics Data System (ADS)

    Park, Chulwoo; Jung, Jinhwan; Lee, Chul Wee; Cho, Joungmo

    2016-12-01

    This paper reports the synthetic route of 3-D network shape α-Fe2O3 from aqueous solutions of iron precursor using a non-ionic polymeric soft-template, Pluronic P123. During the synthesis of α-Fe2O3, particle sizes, crystal phases and morphologies were significantly influenced by pH, concentrations of precursor and template. The unique shape of worm-like hematite was obtained only when a starting solution was prepared by a weakly basic pH condition and a very specific composition of constituents. The synthesized nanocrystal at this condition had a narrow pore size distribution and high surface area compared to the bulk α-Fe2O3 or the one synthesized from lower pH conditions. The hydrocracking performance was tested over the synthesized iron oxide catalysts with different morphologies. The worm-like shape of iron oxide showed a superior performance, including overall yield of liquid fuel product and coke formation, over the hydrocracking of heavy petroleum oil.

  17. Synthesis of Mesoporous α-Fe2O3 Nanoparticles by Non-ionic Soft Template and Their Applications to Heavy Oil Upgrading

    PubMed Central

    Park, Chulwoo; Jung, Jinhwan; Lee, Chul Wee; Cho, Joungmo

    2016-01-01

    This paper reports the synthetic route of 3-D network shape α-Fe2O3 from aqueous solutions of iron precursor using a non-ionic polymeric soft-template, Pluronic P123. During the synthesis of α-Fe2O3, particle sizes, crystal phases and morphologies were significantly influenced by pH, concentrations of precursor and template. The unique shape of worm-like hematite was obtained only when a starting solution was prepared by a weakly basic pH condition and a very specific composition of constituents. The synthesized nanocrystal at this condition had a narrow pore size distribution and high surface area compared to the bulk α-Fe2O3 or the one synthesized from lower pH conditions. The hydrocracking performance was tested over the synthesized iron oxide catalysts with different morphologies. The worm-like shape of iron oxide showed a superior performance, including overall yield of liquid fuel product and coke formation, over the hydrocracking of heavy petroleum oil. PMID:27966663

  18. Synthesis of Mesoporous α-Fe2O3 Nanoparticles by Non-ionic Soft Template and Their Applications to Heavy Oil Upgrading.

    PubMed

    Park, Chulwoo; Jung, Jinhwan; Lee, Chul Wee; Cho, Joungmo

    2016-12-14

    This paper reports the synthetic route of 3-D network shape α-Fe 2 O 3 from aqueous solutions of iron precursor using a non-ionic polymeric soft-template, Pluronic P123. During the synthesis of α-Fe 2 O 3 , particle sizes, crystal phases and morphologies were significantly influenced by pH, concentrations of precursor and template. The unique shape of worm-like hematite was obtained only when a starting solution was prepared by a weakly basic pH condition and a very specific composition of constituents. The synthesized nanocrystal at this condition had a narrow pore size distribution and high surface area compared to the bulk α-Fe 2 O 3 or the one synthesized from lower pH conditions. The hydrocracking performance was tested over the synthesized iron oxide catalysts with different morphologies. The worm-like shape of iron oxide showed a superior performance, including overall yield of liquid fuel product and coke formation, over the hydrocracking of heavy petroleum oil.

  19. Morphology, Growth, and Size Limit of Bacterial Cells

    NASA Astrophysics Data System (ADS)

    Jiang, Hongyuan; Sun, Sean X.

    2010-07-01

    Bacterial cells utilize a living peptidoglycan network (PG) to separate the cell interior from the surroundings. The shape of the cell is controlled by PG synthesis and cytoskeletal proteins that form bundles and filaments underneath the cell wall. The PG layer also resists turgor pressure and protects the cell from osmotic shock. We argue that mechanical influences alter the chemical equilibrium of the reversible PG assembly and determine the cell shape and cell size. Using a mechanochemical approach, we show that the cell shape can be regarded as a steady state of a growing network under the influence of turgor pressure and mechanical stress. Using simple elastic models, we predict the size of common spherical and rodlike bacteria. The influence of cytoskeletal bundles such as crescentin and MreB are discussed within the context of our model.

  20. The morphological interaction between the nasal cavity and maxillary sinuses in living humans.

    PubMed

    Holton, Nathan; Yokley, Todd; Butaric, Lauren

    2013-03-01

    To understand how variation in nasal architecture accommodates the need for effective conditioning of respired air, it is necessary to assess the morphological interaction between the nasal cavity and other aspects of the nasofacial skeleton. Previous studies indicate that the maxillary sinuses may play a key role in accommodating climatically induced nasal variation such that a decrease in nasal cavity volume is associated with a concomitant increase in maxillary sinus volume. However, due to conflicting results in previous studies, the precise interaction of the nasal cavity and maxillary sinuses, in humans, is unclear. This is likely due to the prior emphasis on nasal cavity size, whereas arguably, nasal cavity shape is more important with regard to the interaction with the maxillary sinuses. Using computed tomography scans of living human subjects (N=40), the goal of this study is to assess the interaction between nasal cavity form and maxillary sinus volume in European- and African-derived individuals with differences in nasal cavity morphology. First, we assessed whether there is an inverse relationship between nasal cavity and maxillary sinus volumes. Next, we examined the relationship between maxillary sinus volume and nasal cavity shape using multivariate regression. Our results show that there is a positive relationship between nasal cavity and maxillary sinus volume, indicating that the maxillary sinuses do not accommodate variation in nasal cavity size. However, maxillary sinus volume is significantly correlated with variation in relative internal nasal breadth. Thus, the maxillary sinuses appear to be important for accommodating nasal cavity shape rather than size. Copyright © 2013 Wiley Periodicals, Inc.

  1. Excavated substrate modulates growth instability during nest building in ants

    PubMed Central

    Toffin, Etienne; Kindekens, Jonathan; Deneubourg, Jean-Louis

    2010-01-01

    In social insects, the nests of the same species can show a large difference in size and shape. Despite these large variations, the nests share the same substructures, some appearing during nest growth. In ants, the interplay between nest size and digging activity leads to two successive morphological transitions from circular to branched shapes (budding along the perimeter of the circular cavity and tunnelling of the galleries). Like several other self-organized collective behaviours, this phenomenon, as well as the entire nest-digging process, is thought to be modulated by environmental properties. The present study investigates the effect of excavated substrate on the nest morphogenesis and the morphological transitions by using two materials with different cohesions. Here, we show that the two morphological transitions occur more frequently with a cohesive substrate than with a granular one: 96 per cent of cohesive experiments showed both transitions, whereas only 50 per cent did in granular experiments. We found that transitions and excavation cessation follow area–response thresholds: the shape transitions take place and the digging activity stops when the dug area reaches the corresponding threshold values. The shape transition thresholds are lower with the cohesive substrate and that of stopping digging is independent of nest shape and material. According to simulations, the experimental frequencies of transitions found their origin in the competition between transitions and activity cessation and in the difference between the transition threshold values of each substrate. Our results demonstrate how the substrate properties modulate the collective response and lead to various patterns. Considering the non-specific mechanisms at work, such effects of substrate coarseness have their counterparts in various collective behaviours, generating alternative patterns to colonize and exploit the environment. PMID:20410036

  2. Temporal and spatial variation of morphological descriptors for atmospheric aerosols collected in Mexico City

    NASA Astrophysics Data System (ADS)

    China, S.; Mazzoleni, C.; Dubey, M. K.; Chakrabarty, R. K.; Moosmuller, H.; Onasch, T. B.; Herndon, S. C.

    2010-12-01

    We present an analysis of morphological characteristics of atmospheric aerosol collected during the MILAGRO (Megacity Initiative: Local and Global Research Observations) field campaign that took place in Mexico City in March 2006. The sampler was installed on the Aerodyne mobile laboratory. The aerosol samples were collected on nuclepore clear polycarbonate filters mounted in Costar pop-top membrane holders. More than one hundred filters were collected at different ground sites with different atmospheric and geographical characteristics (urban, sub-urban, mountain-top, industrial, etc.) over a month period. Selected subsets of these filters were analyzed for aerosol morphology using a scanning electron microscope and image analysis techniques. In this study we investigate spatial and temporal variations of aerosol shape descriptors, morphological parameters, and fractal dimension. We also compare the morphological results with other aerosol measurements such as aerosol optical properties(scattering and absorption) and size distribution data. Atmospheric aerosols have different morphological characteristics depending on many parameters such as emission sources, atmospheric formation pathways, aging processes, and aerosol mixing state. The aerosol morphology influences aerosol chemical and mechanical interactions with the environment, physical properties, and radiative effects. In this study, ambient aerosol particles have been classified in different shape groups as spherical, irregularly shaped, and fractal-like aggregates. Different morphological parameters such as aspect ratio, roundness, feret diameter, etc. have been estimated for irregular shaped and spherical particles and for different kinds of soot particles including fresh soot, collapsed and coated soot. Fractal geometry and image processing have been used to obtain morphological characteristics of different soot particles. The number of monomers constituting each aggregate and their diameters were measured and used to estimate an ensemble three-dimensional (3-d) fractal dimension. One-dimensional (1-d) and two-dimensional (2-d) fractal geometries have been measured using a power-law scaling relationship between 1-d and 2-d properties of projected images. Temporal variations in fractal dimension of soot-like aggregates have been observed at the mountaintop site and spatial variation of fractal dimension and other morphological descriptors of different shaped particles have been investigated for the different ground sites.

  3. Three dimensional geometric modeling of processing-tomatoes

    USDA-ARS?s Scientific Manuscript database

    Characterizing tomato geometries with different shapes and sizes would facilitate the design of tomato processing equipments and promote computer-based engineering simulations. This research sought to develop a three-dimensional geometric model that can describe the morphological attributes of proce...

  4. Structural and Morphological Evaluation of Nano-Sized MoSi2 Powder Produced by Mechanical Milling

    NASA Astrophysics Data System (ADS)

    Sameezadeh, Mahmood; Farhangi, Hassan; Emamy, Masoud

    Nano-sized intermetallic powders have received great attention owing to their property advantages over conventional micro-sized counterparts. In the present study nano-sized MoSi2 powder has been produced successfully from commercially available MoSi2 (3 μm) by a mechanical milling process carried out for a period of 100 hours. The effects of milling time on size and morphology of the powders were studied by SEM and TEM and image analyzing system. The results indicate that the as-received micrometric powder with a wide size distribution of irregular shaped morphology changes to a narrow size distribution of nearly equiaxed particles with the progress of attrition milling up to 100 h, reaching an average particle size of 71 nm. Structural evolution of milled samples was characterized by XRD to determine the crystallite size and lattice microstrain using Williamson-Hall method. According to the results, the crystallite size of the powders decreases continuously down to 23 nm with increasing milling time up to 100 h and this size refinement is more rapid at the early stages of the milling process. On the other hand, the lattice strain increases considerably with milling up to 65 h and further milling causes no significant changes of lattice strain.

  5. The effect of environmental uncertainty on morphological design and fluid balance in Sarracenia purpurea L.

    PubMed

    Kingsolver, Joel

    1981-03-01

    To explore principles of organismic design in fluctuating environments, morphological design of the leaf of the pitcher-plant, Sarracenia purpurea, was studied for a population in northern Michigan. The design criterion focused upon the leaf shape and minimum size which effectively avoids leaf desiccation (complete loss of fluid from the leaf cavity) in the face of fluctuating rainfall and meteorological conditions. Bowl- and pitcher-shaped leaves were considered. Simulations show that the pitcher geometry experiences less frequent desiccation than bowls of the same size. Desiccation frequency is inversely related to leaf size; the size distribution of pitcher leaves in the field shows that the majority of pitchers desiccate only 1-3 times per season on average, while smaller pitchers may average up to 8 times per season. A linear filter model of an organism in a fluctuating environment is presented, in which the organism selectively filters the temporal patterns of environmental input. General measures of rainfall predictability based upon information theory and spectral analysis are consistent with the model of a pitcher leaf as a low-pass (frequency) filter which avoids desiccation by eliminating high-frequency rainfall variability.

  6. Shape of the dilated aorta in children with bicuspid aortic valve

    PubMed Central

    Mart, Christopher R; McNerny, Bryn E

    2013-01-01

    Background: The dilated aorta in adults with bicuspid aortic valve has been shown to have different shapes, but it is not known if this occurs in children. This observational study was performed to determine if there are different shapes of the dilated aorta in children with bicuspid aortic valve and their association with age, gender, hemodynamic alterations, and degree of aortic enlargement. Methods: One hundred and eighty-seven echocardiograms done on pediatric patients (0 – 18 years) for bicuspid aortic valve, during 2008, were reviewed. Aortic valve morphology, shape/size of the aorta, and pertinent hemodynamic alterations were documented. Aortic dilation was felt to be present when at least one aortic segment had a z-score > 2.0; global aortic enlargement was determined by summing the aortic segment z-scores. The aortic shape was assessed by age, gender, valve morphology, and hemodynamic alterations. Results: Aortic dilation was present in 104/187 patients. The aorta had six different shapes designated from S1 through S6. There was no association between the aortic shape and gender, aortic valve morphology, or hemodynamic abnormalities. S3 was the most common after the age of six years and was associated with the most significant degree of global aortic enlargement. Conclusions: The shape of the dilated aorta in children with bicuspid aortic valve does not occur in a uniform manner and multiple shapes are seen. S2 and S3 are most commonly seen. As aortic dilation becomes more significant, a single shape (S3) becomes the dominant pattern. PMID:24688228

  7. What does it mean to be pseudo single domain? Demystifying the PSD state

    NASA Astrophysics Data System (ADS)

    Lascu, I.; Harrison, R. J.; Einsle, J. F.; Ball, M.

    2016-12-01

    Until recently, non-interacting stable single domain grains were thought to be the sole reliable paleomagnetic recorders. However most natural samples contain so-called "non-ideal" paleomagnetic recorders, which are either interacting single domain particles, or magnetic grains larger than single domain grains, but smaller than proper multi domain grains, which are poor paleomagnetic recorders. The grain size range for these recorders, which for magnetite comprises grains from 100 nm to a few μm in size, is known as the pseudo single domain (PSD) state. Natural samples containing abundant PSD grains have been shown time and again to reliably record thermomagnetic remanent magnetizations that are stable over billions of years. Here we attempt to shed new light on the PSD state by investigating obsidian varieties found at Glass Butte, Oregon, which present the opportunity to study simple cases of magnetic grains encapsulated in volcanic glass. We do this by combining rock magnetism, scanning electron microscopy (SEM) nanotomography, and finite-element micromagnetic modeling. Using rock magnetism we have identified PSD signatures in these samples via their fingerprint in first-order reversal curve (FORC) diagrams. Tomographic reconstructions obtained by stacking SEM images acquired via sequential milling through sample volumes of a few tens of cubic μm reveal the presence of abundant grains that span the PSD grain size interval. These grains have a variety of shapes, from simple ellipsoidal particles, to more complex morphologies attained through the coalescence of neighboring grains during crystallization, to intricate "rolling snowball" morphologies in larger grains that contain appendices formed as a result of particle growth in a dynamic environment as the flowing lava cooled. Micromagnetic modeling of the simplest morphologies reveals that these grains are in single vortex states, with the remanence controlled by irregularities in grain morphology. Coalesced grains present extreme cases of shape anisotropy, which will control the remanence. The remanence of the largest grains is controlled by the collection of PSD states from areas of the grain with pronounced shape anisotropy. Finally, micromagnetic modeling of realistic grain shapes allows the understanding of PSD signatures in FORC diagrams.

  8. Morphology of the tympanic-basicranial region in Mirounga leonina (Phocidae, Carnivora), postnatal ontogeny and sexual dimorphism

    PubMed Central

    Loza, C M; Scarano, A C; Soibelzon, L H; Negrete, J; Carlini, A A

    2015-01-01

    The auditory region of pinnipeds has seldom been described. Here we describe and analyze the ontogenetic trajectory of the tympanic bulla of the southern elephant seal, Mirounga leonina (Phocidae, Mammalia). This species is extremely sexually dimorphic and highly polygynous (organized in harems). We examined 118 specimens, arranged in three age classes (CI, CII, and CIII), ranging from newborn to adults (males and females). To analyze the overall size and shape of the tympanic bulla we performed a geometric morphometric analysis including 87 skulls. Females reach definitive shape and size of the bulla at earlier ontogenetic stages than males, in agreement with their earlier involvement in reproductive activities. The internal anatomy of the tympanic region (e.g. form and extension of the paries) does not show remarkable differences between sexes or age classes. The greatest differences between age classes are related to bone thickness, resulting from the apposition of new annual layers. An examination of possible sex-related external differences among age classes shows significant shape differences between males and females in CIII. The morphology observed in neonates is conserved across all individuals from CI, which included specimens up to 1 year old. Clear morphological differences were observed between CI individuals, on one hand, and CII individuals plus CIII females on the other. During cranial development of both male and females, the glenoid cavity expands and compresses the bulla; this condition reaches its maximum expression in CIII males. CIII males showed the greatest morphological differences, with respect to both CI and CII individuals, and CIII females. PMID:25827162

  9. The influence of aspect and altitude on the size, shape and spatial distribution of glacial cirques in the High Tatras (Slovakia, Poland)

    NASA Astrophysics Data System (ADS)

    Křížek, Marek; Mida, Peter

    2013-09-01

    The shape of glacial cirques is generally considered to result from the combined actions of climate, topography, and geology. The goal of this article was to determine the dependence of cirque morphology on mesoclimatic conditions defined by altitude and aspect in the highest part of the Carpathians — the High Tatras. The morphology of each of 116 analyzed cirques was described using a set of 12 morphometric characteristics. The relationships between the obtained data were evaluated using simple and multivariate statistical methods. The results indicate that cirques in the High Tatras are scale-specific landforms with allometric development. Their occurrence increases with altitude, but their size decreases. The shape of the cirques is determined by altitude only to a small extent, with the exception of an increase in the degree of incision with altitude. The spatial distribution of cirques is negatively influenced by incoming solar radiation and positively influenced by moisture sources, which came mainly from the NW to N during the cold phases of the Pleistocene. For this reason, north-facing cirques have proportionally stronger representation and are more incised with steep slopes. Thus, cirques have proportionally stronger representation on the northern slopes and represent more developed glacial erosion landforms than those on the southern slopes. Although some relationships were detected between cirque morphology and mesoclimatic factors (such as altitude and aspect), a general discriminant analysis showed that these environmental factors did not explain variations in cirque morphology with sufficient cogency.

  10. Pheromone production, male abundance, body size, and the evolution of elaborate antennae in moths

    PubMed Central

    Symonds, Matthew RE; Johnson, Tamara L; Elgar, Mark A

    2012-01-01

    The males of some species of moths possess elaborate feathery antennae. It is widely assumed that these striking morphological features have evolved through selection for males with greater sensitivity to the female sex pheromone, which is typically released in minute quantities. Accordingly, females of species in which males have elaborate (i.e., pectinate, bipectinate, or quadripectinate) antennae should produce the smallest quantities of pheromone. Alternatively, antennal morphology may be associated with the chemical properties of the pheromone components, with elaborate antennae being associated with pheromones that diffuse more quickly (i.e., have lower molecular weights). Finally, antennal morphology may reflect population structure, with low population abundance selecting for higher sensitivity and hence more elaborate antennae. We conducted a phylogenetic comparative analysis to test these explanations using pheromone chemical data and trapping data for 152 moth species. Elaborate antennae are associated with larger body size (longer forewing length), which suggests a biological cost that smaller moth species cannot bear. Body size is also positively correlated with pheromone titre and negatively correlated with population abundance (estimated by male abundance). Removing the effects of body size revealed no association between the shape of antennae and either pheromone titre, male abundance, or mean molecular weight of the pheromone components. However, among species with elaborate antennae, longer antennae were typically associated with lower male abundances and pheromone compounds with lower molecular weight, suggesting that male distribution and a more rapidly diffusing female sex pheromone may influence the size but not the general shape of male antennae. PMID:22408739

  11. Influence of solvent on micellar morphologies of semifluorinated block copolymers.

    PubMed

    Lee, Min Young; Kim, Sang Jae; Jeong, Yeon Tae; Kim, Joo Hyun; Gal, Yeong-Soon; Lim, Kwon Taek

    2009-12-01

    The influence of solvents on micellar architectures of block copolymers composed of poly(1H,1H-dihydroperfluorooctyl methacrylate) and poly(ethylene oxide) was investigated. In this study, binary solvents with desired proportions were chosen, which had remarkable influence on the morphology of the resulting micelles. With simple adjusting the composition of the binary solvent of chloroform and trichlorofluoromethane, interesting shapes of micelle-like aggregates, such as core-shell, cylinder, worm-like and inverted micelles were formed with sizes of 15, 70, 30 and 250 nm, respectively. In the case of methanol/water system, core-shell spheres and vesicles were produced by varying the proportion of the contents. The morphologies were also tuned to honeycomb-like and bowl-shaped micelles as well as large planar lamellae with holes in DMF and water binary solvent.

  12. Competing Classical and Quantum Effects in Shape Relaxation of a Metallic Island

    NASA Technical Reports Server (NTRS)

    Okamoto, Rowland H.; Chen, D.; Yamada, T.

    2002-01-01

    Pb islands grown on a silicon substrate transform at room temperature from the initially flattop facet geometry into an unusual ring, shape with a volume-preserving mass transport process catalysed by the tip electrical field of a scanning tunnelling microscope. The formation of such ring shape morphology results from the competing classical and quantum effects in the shape relaxation. The latter also leads to a sequential regrowth on alternating, strips of the same facet defined by the underlying substrate steps, showing for the first time the dynamical impact of the quantum size effect on the stability of a nanostructure.

  13. Size and shape variations of the bony components of sperm whale cochleae.

    PubMed

    Schnitzler, Joseph G; Frédérich, Bruno; Früchtnicht, Sven; Schaffeld, Tobias; Baltzer, Johannes; Ruser, Andreas; Siebert, Ursula

    2017-04-25

    Several mass strandings of sperm whales occurred in the North Sea during January and February 2016. Twelve animals were necropsied and sampled around 48 h after their discovery on German coasts of Schleswig Holstein. The present study aims to explore the morphological variation of the primary sensory organ of sperm whales, the left and right auditory system, using high-resolution computerised tomography imaging. We performed a quantitative analysis of size and shape of cochleae using landmark-based geometric morphometrics to reveal inter-individual anatomical variations. A hierarchical cluster analysis based on thirty-one external morphometric characters classified these 12 individuals in two stranding clusters. A relative amount of shape variation could be attributable to geographical differences among stranding locations and clusters. Our geometric data allowed the discrimination of distinct bachelor schools among sperm whales that stranded on German coasts. We argue that the cochleae are individually shaped, varying greatly in dimensions and that the intra-specific variation observed in the morphology of the cochleae may partially reflect their affiliation to their bachelor school. There are increasing concerns about the impact of noise on cetaceans and describing the auditory periphery of odontocetes is a key conservation issue to further assess the effect of noise pollution.

  14. Micron-scale pattern formation in prestressed polygonal films

    NASA Astrophysics Data System (ADS)

    Annabattula, R. K.; Onck, P. R.

    2011-02-01

    In this paper we explore the spontaneous formation of micropatterns in thin prestressed polygonal films using finite element simulations. We study films with different size, thickness, and shape, including square, rectangular, pentagonal, and hexagonal films. Patterns form when the films release the internal eigenstrain by buckling-up, after which the films bond-back to the substrate. After an initial symmetric evolution of the buckling profile, the symmetry of the deflection pattern breaks when the wavelength of wriggles near the film edges decreases. During bond back the deflection morphology converges to a fourfold, fivefold, and sixfold ridging pattern for the square, pentagonal and hexagonal films, respectively, showing a close resemblance with experimental film systems of similar size and shape. Rectangular films of large length to width ratio go through a transition in buckling shapes from the initial Euler mode, through the varicose mode into the antisymmetric telephone-cord mode. For all the film shapes, the ratio of the film height to the effective film width scales with the square root of eigenstrain and is independent of thickness. The bond-back mechanism determines the final wrinkle morphology and is governed by the eigenstrain value at the end of the buckling-up stage and the dimensionless parameter (Γ /EWeq)(Weq/t)3, relating the interface energy to the strain energy in the film.

  15. Bite of the cats: relationships between functional integration and mechanical performance as revealed by mandible geometry.

    PubMed

    Piras, Paolo; Maiorino, Leonardo; Teresi, Luciano; Meloro, Carlo; Lucci, Federico; Kotsakis, Tassos; Raia, Pasquale

    2013-11-01

    Cat-like carnivorous mammals represent a relatively homogeneous group of species whose morphology appears constrained by exclusive adaptations for meat eating. We present the most comprehensive data set of extant and extinct cat-like species to test for evolutionary transformations in size, shape and mechanical performance, that is, von Mises stress and surface traction, of the mandible. Size and shape were both quantified by means of geometric morphometrics, whereas mechanical performance was assessed applying finite element models to 2D geometry of the mandible. Additionally, we present the first almost complete composite phylogeny of cat-like carnivorans for which well-preserved mandibles are known, including representatives of 35 extant and 59 extinct species of Felidae, Nimravidae, and Barbourofelidae. This phylogeny was used to test morphological differentiation, allometry, and covariation of mandible parts within and among clades. After taking phylogeny into account, we found that both allometry and mechanical variables exhibit a significant impact on mandible shape. We also tested whether mechanical performance was linked to morphological integration. Mechanical stress at the coronoid process is higher in sabertoothed cats than in any other clade. This is strongly related to the high degree of covariation within modules of sabertooths mandibles. We found significant correlation between integration at the clade level and per-clade averaged stress values, on both original data and by partialling out interclade allometry from shapes when calculating integration. This suggests a strong interaction between natural selection and the evolution of developmental and functional modules at the clade level.

  16. Synthesis of silver nano-materials from Grevillea robusta A Cunn (Silver-oak tree) leaves extract and shape directing role of cetyltrimethylammonium bromide

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ahmad, Rabia; Faisal, Qamer; Hussain, Sajjad

    Grevillea robusta (Silver-oak tree) tree is a medicinal tree. Conventional UV-visible spectrophotometric and transmission electron microscopic technique were used to determine the morphology of silver nanoplates (AgNP) using Grevillea robusta (Silver-oak tree) aqueous leaves extract for the first time. The visible spectra showed the presence of three well defined surface plasmon absorption (SPR) bands at 500, 550 and 675 nm which was attributed to the anisotropic growth of Ag-nanoplates. Transmission electron microscopic (TEM) analysis of AgNP showed formation of truncated triangular, polyhedral with some irregular shapes nanoplates in the size range 8-20 nm. Cetyltrimethylammonium bromide (CTAB) has no significant effect on themore » shape of the spectra, position of SPR bands, size and size distribution of AgNP.« less

  17. Two new Myxidium species (Myxosporea: Myxidiidae) infecting the gallbladder of African flying fish, Cheilopogon nigricans and Suez fusilier, Caesio suevicus from the Red Sea, Egypt: a morphological and morphometric study.

    PubMed

    Abdel-Baki, Abdel-Azeem Sh

    2009-08-01

    Myxidium maamouni sp. n. and Myxidium aydai sp. n. were described from the gallbladder of the African flying fish Cheilopogon nigricans and Suez fusilier Caesio suevicus, respectively. Fishes were collected from the Red Sea at Al-Quseir, Egypt. M. maamouni have irregular to mostly rounded polysporous plasmodia with diameter of 27 microm. Spores were sigmoid or S-shaped and sometimes spindle-shaped in the frontal view with smooth valves. They measured 13.5 x 8.0 x 8.2 microm in size. Their polar capsules were equal pyriform and measured 7.0 x 3.2 microm in size with nine to 12 coils. Spores of M. aydai were spindle-shaped in the frontal view with thin smooth valves. They measured 23.0 x 5.6 x 5.5 microm in size. Their polar capsules were pyriform and measured 7.2 x 3.4 microm in size with eight to nine coils.

  18. WormSizer: high-throughput analysis of nematode size and shape.

    PubMed

    Moore, Brad T; Jordan, James M; Baugh, L Ryan

    2013-01-01

    The fundamental phenotypes of growth rate, size and morphology are the result of complex interactions between genotype and environment. We developed a high-throughput software application, WormSizer, which computes size and shape of nematodes from brightfield images. Existing methods for estimating volume either coarsely model the nematode as a cylinder or assume the worm shape or opacity is invariant. Our estimate is more robust to changes in morphology or optical density as it only assumes radial symmetry. This open source software is written as a plugin for the well-known image-processing framework Fiji/ImageJ. It may therefore be extended easily. We evaluated the technical performance of this framework, and we used it to analyze growth and shape of several canonical Caenorhabditis elegans mutants in a developmental time series. We confirm quantitatively that a Dumpy (Dpy) mutant is short and fat and that a Long (Lon) mutant is long and thin. We show that daf-2 insulin-like receptor mutants are larger than wild-type upon hatching but grow slow, and WormSizer can distinguish dauer larvae from normal larvae. We also show that a Small (Sma) mutant is actually smaller than wild-type at all stages of larval development. WormSizer works with Uncoordinated (Unc) and Roller (Rol) mutants as well, indicating that it can be used with mutants despite behavioral phenotypes. We used our complete data set to perform a power analysis, giving users a sense of how many images are needed to detect different effect sizes. Our analysis confirms and extends on existing phenotypic characterization of well-characterized mutants, demonstrating the utility and robustness of WormSizer.

  19. Evidence of selection on phenotypic plasticity and cost of plasticity in response to host-feeding sources in the major Chagas disease vector Triatoma infestans.

    PubMed

    Nattero, Julieta; Leonhard, Gustavo; Gürtler, Ricardo E; Crocco, Liliana B

    2015-12-01

    Phenotypic plasticity is the ability of a genotype to display alternative phenotypes in different environments. Understanding how plasticity evolves and the factors that favor and constrain its evolution have attracted great interest. We investigated whether selection on phenotypic plasticity and costs of plasticity affect head and wing morphology in response to host-feeding sources in the major Chagas disease vector Triatoma infestans. Full-sib families were assigned to blood-feeding on either live pigeons or guinea pigs throughout their lives. We measured diet-induced phenotypic plasticity on wing and head size and shape; characterized selection on phenotypic plasticity for female and male fecundity rates, and evaluated costs of plasticity. Wing size and shape variables exhibited significant differences in phenotypic plasticity associated with host-feeding source in female and male bugs. Evidence of selection on phenotypic plasticity was detected in head size and shape for guinea pig-fed females. A lower female fecundity rate was detected in more plastic families for traits that showed selection on plasticity. These results provide insights into the morphological phenotypic plasticity of T. infestans, documenting fitness advantages of head size and shape for females fed on guinea pigs. This vector species showed measurable benefits of responding plastically to environmental variation rather than adopting a fixed development plan. The presence of cost of plasticity suggests constraints on the evolution of plasticity. Our study indicates that females fed on guinea pigs (and perhaps on other suitable mammalian hosts) have greater chances of evolving under selection on phenotypic plasticity subject to some constraints. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. Kinetically-controlled template-free synthesis of hollow silica micro-/nanostructures with unusual morphologies

    NASA Astrophysics Data System (ADS)

    Zhang, An-Qi; Li, Hui-Jun; Qian, Dong-Jin; Chen, Meng

    2014-04-01

    We report a kinetically-controlled template-free room-temperature production of hollow silica materials with various novel morphologies, including tubes, crutches, ribbons, bundles and bells. The obtained products, which grew in a well-controlled manner, were monodispersed in shape and size. The role of ammonia, sodium citrate, polyvinylpyrrolidone, chloroauric acid and NaCl in shape control is discussed in detail. The oriented growth of these micro-/nanostructures directed by reverse micelles followed a solution-solution-solid (SSS) mechanism, similar to the classic vapor-liquid-solid mechanism. The evolution processes of silica rods, tubes, crutches, bundles and bells were recorded using transmission electron microscopy to prove the SSS mechanism.

  1. The influence of amphiphilic additional agents on the morphology and photoluminescence properties of calcium carbonate phosphor

    NASA Astrophysics Data System (ADS)

    Mou, Yongren; Kang, Ming; Liu, Min; Wang, Feng; Chen, Kexu; Sun, Rong

    2017-06-01

    In order to investigate the effect of amphiphilic additional agents on the morphology (particle shape, particle size and particle size distribution) and photoluminescence performance of calcium carbonate phosphor, the phosphors AA-CaCO3:Eu3+ (AA = glycerol or sodium dodecyl sulfate) were synthesized by the microwave-assisted co-precipitation method using glycerol (Gly) and sodium dodecyl sulfate (SDS) as amphiphilic additional agents (AA), respectively. The phase structure, morphology and luminescent properties of the as-synthesized samples were characterized by X-ray diffraction, scanning electron microscope, laser diffraction particle size analyzer and Fluorescence spectrophotometer, respectively. The results showed that the phase structure and morphology of AA-CaCO3:Eu3+ changed along with different types and amount of amphiphilic additional agents evidently. The particle size of Gly-CaCO3 decreased to 1.383 µm when the volume ratio reached 8:2 (Gly:H2O). Photoluminescence (PL) spectra show that all the AA-CaCO3:Eu3+ phosphors exhibit strong red emission peak originating from electric-dipole transition 5D0 → 7F2 (614 nm) of Eu3+ ions and the amphiphilic molecules (Gly and SDS) had a huge influence on photoluminescence intensity.

  2. Effect of Particle Morphology on the Reactivity of Explosively Dispersed Titanium Particles

    NASA Astrophysics Data System (ADS)

    Frost, David; Cairns, Malcolm; Goroshin, Samuel; Zhang, Fan

    2009-06-01

    The effect of particle morphology on the reaction of titanium (Ti) particles explosively dispersed during the detonation of either cylindrical or spherical charges has been investigated experimentally. The explosive charges consisted of packed beds of Ti particles saturated with nitromethane. The reaction behavior of irregularly-shaped Ti particles in three size ranges is compared with tests with spherical Ti particles. The particle reaction is strongly dependent on particle morphology, e.g., 95 μm spherical Ti particles failed to ignite (in cylinders up to 49 mm in dia), whereas similarly sized irregular Ti particles readily ignited. For irregular particles, the uniformity of ignition on the particle cloud surface was almost independent of particle size, but depended on charge diameter. As the charge diameter was reduced, ignition in the conically expanding particle cloud occurred only at isolated spots or bands. For spherical charges, although large irregular Ti particles ignited promptly and uniformly throughout the particle cloud, the smallest particles dispersed nonuniformly and ignition occurred at isolated locations. In general, particle ignition is a competition between particle heating (which is influenced by particle morphology, size, number density and the local thermodynamic history) and expansion cooling of the products.

  3. The quintuple-shape memory effect in electrospun nanofiber membranes

    NASA Astrophysics Data System (ADS)

    Zhang, Fenghua; Zhang, Zhichun; Liu, Yanju; Lu, Haibao; Leng, Jinsong

    2013-08-01

    Shape memory fibrous membranes (SMFMs) are an emerging class of active polymers, which are capable of switching from a temporary shape to their permanent shape upon appropriate stimulation. Quintuple-shape memory membranes based on the thermoplastic polymer Nafion, with a stable fibrous structure, are achieved via electrospinning technology, and possess a broad transition temperature. The recovery of multiple temporary shapes of electrospun membranes can be triggered by heat in a single triple-, quadruple-, quintuple-shape memory cycle, respectively. The fiber morphology and nanometer size provide unprecedented design flexibility for the adjustable morphing effect. SMFMs enable complex deformations at need, having a wide potential application field including smart textiles, artificial intelligence robots, bio-medical engineering, aerospace technologies, etc in the future.

  4. Application Of Empirical Phase Diagrams For Multidimensional Data Visualization Of High Throughput Microbatch Crystallization Experiments.

    PubMed

    Klijn, Marieke E; Hubbuch, Jürgen

    2018-04-27

    Protein phase diagrams are a tool to investigate cause and consequence of solution conditions on protein phase behavior. The effects are scored according to aggregation morphologies such as crystals or amorphous precipitates. Solution conditions affect morphological features, such as crystal size, as well as kinetic features, such as crystal growth time. Common used data visualization techniques include individual line graphs or symbols-based phase diagrams. These techniques have limitations in terms of handling large datasets, comprehensiveness or completeness. To eliminate these limitations, morphological and kinetic features obtained from crystallization images generated with high throughput microbatch experiments have been visualized with radar charts in combination with the empirical phase diagram (EPD) method. Morphological features (crystal size, shape, and number, as well as precipitate size) and kinetic features (crystal and precipitate onset and growth time) are extracted for 768 solutions with varying chicken egg white lysozyme concentration, salt type, ionic strength and pH. Image-based aggregation morphology and kinetic features were compiled into a single and easily interpretable figure, thereby showing that the EPD method can support high throughput crystallization experiments in its data amount as well as its data complexity. Copyright © 2018. Published by Elsevier Inc.

  5. Prospects of photonic nanojets for precise exposure on microobjects

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Geints, Yu. E., E-mail: ygeints@iao.ru; Zuev Institute of Atmospheric Optics, SB Russian Academy of Sciences, Acad. Zuev Square 1, Tomsk, 634021; Panina, E. K., E-mail: pek@iao.ru

    We report on the new optical tool for precise manipulation of various microobjects. This tool is referred to as a “photonic nanojet” (PJ) and corresponds to specific spatially localized and high-intensity area formed near micron-sized transparent spherical dielectric particles illuminated by a visible laser radiation The descriptive analysis of the morphological shapes of photonic nanojets is presented. The PJ shape characterization is based on the numerical calculations of the near-field distribution according to the Mie theory and accounts for jet dimensions and shape complexity.

  6. Morphology of single inhalable particle inside public transit biodiesel fueled bus.

    PubMed

    Shandilya, Kaushik K; Kumar, Ashok

    2010-01-01

    In an urban-transit bus, fueled by biodiesel in Toledo, Ohio, single inhalable particle samples in October 2008 were collected and detected by scanning electron microscopy and energy dispersive X-ray spectrometry (SEM/EDS). Particle size analysis found bimodal distribution at 0.2 and 0.5 microm. The particle morphology was characterized by 14 different shape clusters: square, pentagon, hexagon, heptagon, octagon, nonagon, decagon, agglomerate, sphere, triangle, oblong, strip, line or stick, and unknown, by quantitative order. The square particles were common in the samples. Round and triangle particles are more, and pentagon, hexagon, heptagon, octagon, nonagon, decagon, strip, line or sticks are less. Agglomerate particles were found in abundance. The surface of most particles was coarse with a fractal edge that can provide a suitable chemical reaction bed in the polluted atmospheric environment. The three sorts of surface patterns of squares were smooth, semi-smooth, and coarse. The three sorts of square surface patterns represented the morphological characteristics of single inhalable particles in the air inside the bus in Toledo. The size and shape distribution results were compared to those obtained for a bus using ultra low sulfur diesel.

  7. Evolution of eye size and shape in primates.

    PubMed

    Ross, Callum F; Kirk, E Christopher

    2007-03-01

    Strepsirrhine and haplorhine primates exhibit highly derived features of the visual system that distinguish them from most other mammals. Comparative data link the evolution of these visual specializations to the sequential acquisition of nocturnal visual predation in the primate stem lineage and diurnal visual predation in the anthropoid stem lineage. However, it is unclear to what extent these shifts in primate visual ecology were accompanied by changes in eye size and shape. Here we investigate the evolution of primate eye morphology using a comparative study of a large sample of mammalian eyes. Our analysis shows that primates differ from other mammals in having large eyes relative to body size and that anthropoids exhibit unusually small corneas relative to eye size and body size. The large eyes of basal primates probably evolved to improve visual acuity while maintaining high sensitivity in a nocturnal context. The reduced corneal sizes of anthropoids reflect reductions in the size of the dioptric apparatus as a means of increasing posterior nodal distance to improve visual acuity. These data support the conclusion that the origin of anthropoids was associated with a change in eye shape to improve visual acuity in the context of a diurnal predatory habitus.

  8. Effect of Particle Morphology on the Reactivity of Explosively Dispersed Titanium Particles

    NASA Astrophysics Data System (ADS)

    Frost, David L.; Cairns, Malcolm; Goroshin, Samuel; Zhang, Fan

    2009-12-01

    The effect of particle morphology on the reaction of titanium (Ti) particles explosively dispersed during the detonation of either cylindrical or spherical charges has been investigated experimentally. The explosive charges consisted of packed beds of Ti particles saturated with nitromethane. The reaction behaviour of irregularly-shaped Ti particles in three size ranges is compared with tests with spherical Ti particles. The particle reaction is strongly dependent on particle morphology, e.g., 95 μm spherical Ti particles failed to ignite (in cylinders up to 49 mm in dia), whereas similarly sized irregular Ti particles readily ignited. For irregular particles, the uniformity of ignition on the particle cloud surface was almost independent of particle size, but depended on charge diameter. As the charge diameter was reduced, ignition in the conically expanding particle cloud occurred only at isolated spots or bands. For spherical charges, whereas large irregular Ti particles ignited promptly and uniformly throughout the particle cloud, the smallest particles dispersed nonuniformly and ignition occurred at isolated locations after a delay. Hence the charge geometry, as well as particle morphology, influences the reaction behaviour of the particles.

  9. Does skull morphology constrain bone ornamentation? A morphometric analysis in the Crocodylia.

    PubMed

    Clarac, F; Souter, T; Cubo, J; de Buffrénil, V; Brochu, C; Cornette, R

    2016-08-01

    Previous quantitative assessments of the crocodylians' dermal bone ornamentation (this ornamentation consists of pits and ridges) has shown that bone sculpture results in a gain in area that differs between anatomical regions: it tends to be higher on the skull table than on the snout. Therefore, a comparative phylogenetic analysis within 17 adult crocodylian specimens representative of the morphological diversity of the 24 extant species has been performed, in order to test if the gain in area due to ornamentation depends on the skull morphology, i.e. shape and size. Quantitative assessment of skull size and shape through geometric morphometrics, and of skull ornamentation through surface analyses, produced a dataset that was analyzed using phylogenetic least-squares regression. The analyses reveal that none of the variables that quantify ornamentation, be they on the snout or the skull table, is correlated with the size of the specimens. Conversely, there is more disparity in the relationships between skull conformations (longirostrine vs. brevirostrine) and ornamentation. Indeed, both parameters GApit (i.e. pit depth and shape) and OArelat (i.e. relative area of the pit set) are negatively correlated with snout elongation, whereas none of the values quantifying ornamentation on the skull table is correlated with skull conformation. It can be concluded that bone sculpture on the snout is influenced by different developmental constrains than on the skull table and is sensible to differences in the local growth 'context' (allometric processes) prevailing in distinct skull parts. Whatever the functional role of bone ornamentation on the skull, if any, it seems to be restricted to some anatomical regions at least for the longirostrine forms that tend to lose ornamentation on the snout. © 2016 Anatomical Society.

  10. Fracture morphologies of carbon-black-loaded SBR (styrene-butadiene rubber) subjected to low-cycle, high-stress fatigue. [Styrene-butadiene rubber

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Goldberg, A.; Lesuer, D.R.; Patt, J.

    Experimental results, together with an analytical model, related to the loss in tensile strength of styrene-butadiene rubber (SBR) loaded with carbon black (CB) that had been subjected to low-cycle, high-stress fatigue tests were presented in a prior paper. The drop in tensile strength relative to that of a virgin sample was considered to be a measure of damage induced during the fatigue test. The present paper is a continuation of this study dealing with the morphological interpretations of the fractured surfaces, whereby the cyclic-tearing behavior, resulting in the damage, is related to the test and material parameters. It was foundmore » that failure is almost always initiated in the bulk of a sample at a material flaw. The size and definition of a flaw increase with an increase in carbon-black loading. Initiation flaw sites are enveloped by fan-shaped or penny-shaped regions which develop during cycling. The size and morphology of a fatigue-tear region appears to be independent of the fatigue load or the extent of the damage (strength loss). By contrast, either an increase in cycling load or an increase in damage at constant load increases the definition of the fatigue-region morphology for all formulations of carbon-black. On the finest scale, the morphology can be described in terms of tearing of individual groups of rubber strands, collapsing to form a cell-like structure. 18 refs., 13 figs.« less

  11. Scattering and radiative properties of complex soot and soot-containing particles

    NASA Astrophysics Data System (ADS)

    Liu, L.; Mishchenko, M. I.; Mackowski, D. W.; Dlugach, J.

    2012-12-01

    Tropospheric soot and soot containing aerosols often exhibit nonspherical overall shapes and complex morphologies. They can externally, semi-externally, and internally mix with other aerosol species. This poses a tremendous challenge in particle characterization, remote sensing, and global climate modeling studies. To address these challenges, we used the new numerically exact public-domain Fortran-90 code based on the superposition T-matrix method (STMM) and other theoretical models to analyze the potential effects of aggregation and heterogeneity on light scattering and absorption by morphologically complex soot containing particles. The parameters we computed include the whole scattering matrix elements, linear depolarization ratios, optical cross-sections, asymmetry parameters, and single scattering albedos. It is shown that the optical characteristics of soot and soot containing aerosols very much depend on particle sizes, compositions, and aerosol overall shapes. The soot particle configurations and heterogeneities can have a substantial effect that can result in a significant enhancement of extinction and absorption relative to those computed from the Lorenz-Mie theory. Meanwhile the model calculated information combined with in-situ and remote sensed data can be used to constrain soot particle shapes and sizes which are much needed in climate models.

  12. Surfactant-Free Shape Control of Gold Nanoparticles Enabled by Unified Theoretical Framework of Nanocrystal Synthesis.

    PubMed

    Wall, Matthew A; Harmsen, Stefan; Pal, Soumik; Zhang, Lihua; Arianna, Gianluca; Lombardi, John R; Drain, Charles Michael; Kircher, Moritz F

    2017-06-01

    Gold nanoparticles have unique properties that are highly dependent on their shape and size. Synthetic methods that enable precise control over nanoparticle morphology currently require shape-directing agents such as surfactants or polymers that force growth in a particular direction by adsorbing to specific crystal facets. These auxiliary reagents passivate the nanoparticles' surface, and thus decrease their performance in applications like catalysis and surface-enhanced Raman scattering. Here, a surfactant- and polymer-free approach to achieving high-performance gold nanoparticles is reported. A theoretical framework to elucidate the growth mechanism of nanoparticles in surfactant-free media is developed and it is applied to identify strategies for shape-controlled syntheses. Using the results of the analyses, a simple, green-chemistry synthesis of the four most commonly used morphologies: nanostars, nanospheres, nanorods, and nanoplates is designed. The nanoparticles synthesized by this method outperform analogous particles with surfactant and polymer coatings in both catalysis and surface-enhanced Raman scattering. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Evaluation of bovine hemimandible morphology by means of elliptic Fourier descriptors.

    PubMed

    Parés-Casanova, Pere M

    2014-01-01

    The aim of this research was to compare size (area) and shape variations of bovine hemimandibles according to age. Digital photographs were obtained for 34 hemimandibles belonging to different European breeds of cattle. The specimens were classified according to age, as determined by molar eruption: b months ("young", M1 erupting, n = 8), 10 months ("immature", M2 erupting, n = 9) and over 24 months ("adult", M3 fully erupted, n = 17). Captured images were then digitally analysed based on elliptic Fourier descriptors, which mathematically characterise the area and shape. Hemimandibular areas only showed significant differences between the adults (2752.3 cm2 +/- 250.4) and young subjects (2373.8 cm2 +/- 300.2). The areas for each age group were not linked to linear shape modifications, which was the same for all age groups. So, bovine hemimandibular form change is mainly related to size changes. Shape variability is centred on the condylar ramus.

  14. Variation of telencephalon morphology of the threespine stickleback (Gasterosteus aculeatus) in relation to inferred ecology.

    PubMed

    Park, Peter J; Bell, M A

    2010-06-01

    We tested the hypothesis that increased telencephalon size has evolved in threespine stickleback fish (Gasterosteus aculeatus) from structurally complex habitats using field-caught samples from one sea-run (ancestral) and 18 ecologically diverse freshwater (descendant) populations. Freshwater habitats ranged from shallow, structurally complex lakes with benthic-foraging stickleback (benthics), to deeper, structurally simple lakes in which stickleback depend more heavily on plankton for prey (generalists). Contrary to our expectations, benthics had smaller telencephala than generalists, but the shape of the telencephalon of the sea-run and benthic populations were more convex laterally. Convex telencephalon shape may indicate enlargement of the dorsolateral region, which is homologous with the tetrapod hippocampus. Telencephalon morphology is also sexually dimorphic, with larger, less convex telencephala in males. Freshwater stickleback from structurally complex habitats have retained the ancestral telencephalon morphology, but populations that feed more in open habitats on plankton have evolved larger, laterally concave telencephala.

  15. Pollen Morphology of Caesalpinia pulcherrima (L.) Swartz in Highland and Lowland West Sumatra

    NASA Astrophysics Data System (ADS)

    Fitri, R.; Des, M.

    2018-04-01

    Determine the morphology structure of pollen on some variation colour of corolla Caesalpinia pulcherrima L. (Swartz) in highland and lowland West Sumatra has been conducted. The result reveals that topography and variation colour of corolla C. pulcherrima L. (Swartz) affects the shape of pollen. Pollen of C. pulcherrima L. (Swartz) has single grains or monad, isopolar polarity, radial symmetry, and size categories large. The length of polar axis (P) 58.16 to 74.11 μm, the length of the equatorial diameter (E) 59.86 to 75.97 μm, so that pollen can be classified into sub-spheroidal sub-oblate, spheriodal sub-spheroidal oblate, and sub-spheroidal prolate. Ornamentation of C. pulcherrima (L.) Swartz was reticulate. The pollen has aperture 3, the type pore and located in equatorial. From these data can be concluded that pollen from varying colour of corolla C. pulcherrima (L.) Swartz has same in terms of unit, polarity, symmetry, size, and type aperture, but it different in terms of shape.

  16. Bentho-pelagic divergence of cichlid feeding architecture was prodigious and consistent during multiple adaptive radiations within African rift-lakes.

    PubMed

    Cooper, W James; Parsons, Kevin; McIntyre, Alyssa; Kern, Brittany; McGee-Moore, Alana; Albertson, R Craig

    2010-03-08

    How particular changes in functional morphology can repeatedly promote ecological diversification is an active area of evolutionary investigation. The African rift-lake cichlids offer a calibrated time series of the most dramatic adaptive radiations of vertebrate trophic morphology yet described, and the replicate nature of these events provides a unique opportunity to test whether common changes in functional morphology have repeatedly facilitated their ecological success. Specimens from 87 genera of cichlid fishes endemic to Lakes Tanganyka, Malawi and Victoria were dissected in order to examine the functional morphology of cichlid feeding. We quantified shape using geometric morphometrics and compared patterns of morphological diversity using a series of analytical tests. The primary axes of divergence were conserved among all three radiations, and the most prevalent changes involved the size of the preorbital region of the skull. Even the fishes from the youngest of these lakes (Victoria), which exhibit the lowest amount of skull shape disparity, have undergone extensive preorbital evolution relative to other craniofacial traits. Such changes have large effects on feeding biomechanics, and can promote expansion into a wide array of niches along a bentho-pelagic ecomorphological axis. Here we show that specific changes in trophic anatomy have evolved repeatedly in the African rift lakes, and our results suggest that simple morphological alterations that have large ecological consequences are likely to constitute critical components of adaptive radiations in functional morphology. Such shifts may precede more complex shape changes as lineages diversify into unoccupied niches. The data presented here, combined with observations of other fish lineages, suggest that the preorbital region represents an evolutionary module that can respond quickly to natural selection when fishes colonize new lakes. Characterizing the changes in cichlid trophic morphology that have contributed to their extraordinary adaptive radiations has broad evolutionary implications, and such studies are necessary for directing future investigations into the proximate mechanisms that have shaped these spectacular phenomena.

  17. Cranial airways and the integration between the inner and outer facial skeleton in humans.

    PubMed

    Bastir, Markus; Rosas, Antonio

    2013-10-01

    The cranial airways are in the center of the human face. Therefore variation in the size and shape of these central craniofacial structures could have important consequences for the surrounding midfacial morphology during development and evolution. Yet such interactions are unclear because one school of thought, based on experimental and developmental evidence, suggests a relative independence (modularity) of these two facial compartments, whereas another one assumes tight morphological integration. This study uses geometric morphometrics of modern humans (N = 263) and 40 three-dimensional-landmarks of the skeletal nasopharynx and nasal cavity and outer midfacial skeleton to analyze these questions in terms of modularity. The sizes of all facial compartments were all strongly correlated. Shape integration was high between the cranial airways and the outer midfacial skeleton and between the latter and the anterior airway openings (skeletal regions close to and including piriform aperture). However, no shape integration was detected between outer midface and posterior airway openings (nasopharynx and choanae). Similarly, no integration was detected between posterior and anterior airway openings. This may reflect functional modularization of nasal cavity compartments related to respiratory physiology and differential developmental interactions with the face. Airway size likely relates to the energetics of the organism, whereas airways shape might be more indicative of respiratory physiology and climate. Although this hypothesis should be addressed in future steps, here we suggest that selection on morphofunctional characteristics of the cranial airways could have cascading effects for the variation, development, and evolution of the human face. Copyright © 2013 Wiley Periodicals, Inc.

  18. Morphological evaluation of clefts of the lip, palate, or both in dogs.

    PubMed

    Peralta, Santiago; Fiani, Nadine; Kan-Rohrer, Kimi H; Verstraete, Frank J M

    2017-08-01

    OBJECTIVE To systematically characterize the morphology of cleft lip, cleft palate, and cleft lip and palate in dogs. ANIMALS 32 client-owned dogs with clefts of the lip (n = 5), palate (23), or both (4) that had undergone a CT or cone-beam CT scan of the head prior to any surgical procedures involving the oral cavity or face. PROCEDURES Dog signalment and skull type were recorded. The anatomic form of each defect was characterized by use of a widely used human oral-cleft classification system on the basis of CT findings and clinical images. Other defect morphological features, including shape, relative size, facial symmetry, and vomer involvement, were also recorded. RESULTS 9 anatomic forms of cleft were identified. Two anatomic forms were identified in the 23 dogs with cleft palate, in which differences in defect shape and size as well as vomer abnormalities were also evident. Seven anatomic forms were observed in 9 dogs with cleft lip or cleft lip and palate, and most of these dogs had incisive bone abnormalities and facial asymmetry. CONCLUSIONS AND CLINICAL RELEVANCE The morphological features of congenitally acquired cleft lip, cleft palate, and cleft lip and palate were complex and varied among dogs. The features identified here may be useful for surgical planning, developing of clinical coding schemes, or informing genetic, embryological, or clinical research into birth defects in dogs and other species.

  19. Growth trajectories in the cave bear and its extant relatives: an examination of ontogenetic patterns in phylogeny.

    PubMed

    Fuchs, Manuela; Geiger, Madeleine; Stange, Madlen; Sánchez-Villagra, Marcelo R

    2015-11-02

    The study of postnatal ontogeny can provide insights into evolution by offering an understanding of how growth trajectories have evolved resulting in adult morphological disparity. The Ursus lineage is a good subject for studying cranial and mandibular shape and size variation in relation to postnatal ontogeny and phylogeny because it is at the same time not diverse but the species exhibit different feeding ecologies. Cranial and mandibular shapes of Ursus arctos (brown bear), U. maritimus (polar bear), U. americanus (American black bear), and the extinct U. spelaeus (cave bear) were examined, using a three-dimensional geometric morphometric approach. Additionally, ontogenetic series of crania and mandibles of U. arctos and U. spelaeus ranging from newborns to senile age were sampled. The distribution of specimens in morphospace allowed to distinguish species and age classes and the ontogenetic trajectories U. arctos and U. spelaeus were found to be more similar than expected by chance. Cranial shape changes during ontogeny are largely size related whereas the evolution of cranial shape disparity in this clade appears to be more influenced by dietary adaptation than by size and phylogeny. The different feeding ecologies are reflected in different cranial and mandibular shapes among species. The cranial and mandibular shape disparity in the Ursus lineage appears to be more influenced by adaptation to diet than by size or phylogeny. In contrast, the cranial and mandibular shape changes during postnatal ontogeny in U. arctos and U. spelaeus are probably largely size related. The patterns of morphospace occupation of the cranium and the mandible in adults and through ontogeny are different.

  20. Cocklebur-shaped colloidal dispersions.

    PubMed

    Lestage, David J; Urban, Marek W

    2005-11-08

    Unique cocklebur-shaped colloidal dispersions were prepared using a combination of a nanoextruder applied to the aqueous solution containing methyl methacrylate (MMA) and n-butyl acrylate (n-BA) with azo-bis-isobutyronitrile (AIBN) or potassium persulfate (KPS) initiators and stabilized by a mixture of sodium dioctyl sulfosuccinate (SDOSS) and 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DCPC) phospholipid. Upon extrusion and heating to 75 degrees C, methyl methacrylate/n-butyl acrylate (MMA/nBA) colloidal particles containing tubules pointing outward were obtained as a result of DCPC phospholipids present at the particle surfaces. The same cocklebur-shaped particles were obtained when classical polymerization was used without a nanoextruder under similar compositional and thermal conditions, giving a particle size of 159 nm. However, when Ca(2+) ions are present during polymerization, cocklebur morphologies are disrupted. Because DCPC tubules undergo a transition at 38 degrees C, such cocklebur morphologies may offer numerous opportunities for devices with stimuli-responsive characteristics.

  1. Specific-age group sex estimation of infants through geometric morphometrics analysis of pubis and ischium.

    PubMed

    Estévez Campo, Enrique José; López-Lázaro, Sandra; López-Morago Rodríguez, Claudia; Alemán Aguilera, Inmaculada; Botella López, Miguel Cecilio

    2018-05-01

    Sex determination of unknown individuals is one of the primary goals of Physical and Forensic Anthropology. The adult skeleton can be sexed using both morphological and metric traits on a large number of bones. The human pelvis is often used as an important element of adult sex determination. However, studies carried out about the pelvic bone in subadult individuals present several limitations due the absence of sexually dimorphic characteristics. In this study, we analyse the sexual dimorphism of the immature pubis and ischium bones, attending to their shape (Procrustes residuals) and size (centroid size), using an identified sample of subadult individuals composed of 58 individuals for the pubis and 83 for the ischium, aged between birth and 1year of life, from the Granada osteological collection of identified infants (Granada, Spain). Geometric morphometric methods and discriminant analysis were applied to this study. The results of intra- and inter-observer error showed good and excellent agreement in the location of coordinates of landmarks and semilandmarks, respectively. Principal component analysis performed on shape and size variables showed superposition of the two sexes, suggesting a low degree of sexual dimorphism. Canonical variable analysis did not show significant changes between the male and female shapes. As a consequence, discriminant analysis with leave-one-out cross validation provided low classification accuracy. The results suggested a low degree of sexual dimorphism supported by significant sexual dimorphism in the subadult sample and poor cross-validated classification accuracy. The inclusion of centroid size as a discriminant variable does not imply a significant improvement in the results of the analysis. The similarities found between the sexes prevent consideration of pubic and ischial morphology as a sex estimator in early stages of development. The authors suggest extending this study by analysing the different trajectories of shape and size in later ontogeny between males and females. Copyright © 2018 Elsevier B.V. All rights reserved.

  2. Comparative morphology of changeable skin papillae in octopus and cuttlefish.

    PubMed

    Allen, Justine J; Bell, George R R; Kuzirian, Alan M; Velankar, Sachin S; Hanlon, Roger T

    2014-04-01

    A major component of cephalopod adaptive camouflage behavior has rarely been studied: their ability to change the three-dimensionality of their skin by morphing their malleable dermal papillae. Recent work has established that simple, conical papillae in cuttlefish (Sepia officinalis) function as muscular hydrostats; that is, the muscles that extend a papilla also provide its structural support. We used brightfield and scanning electron microscopy to investigate and compare the functional morphology of nine types of papillae of different shapes, sizes and complexity in six species: S. officinalis small dorsal papillae, Octopus vulgaris small dorsal and ventral eye papillae, Macrotritopus defilippi dorsal eye papillae, Abdopus aculeatus major mantle papillae, O. bimaculoides arm, minor mantle, and dorsal eye papillae, and S. apama face ridge papillae. Most papillae have two sets of muscles responsible for extension: circular dermal erector muscles arranged in a concentric pattern to lift the papilla away from the body surface and horizontal dermal erector muscles to pull the papilla's perimeter toward its core and determine shape. A third set of muscles, retractors, appears to be responsible for pulling a papilla's apex down toward the body surface while stretching out its base. Connective tissue infiltrated with mucopolysaccharides assists with structural support. S. apama face ridge papillae are different: the contraction of erector muscles perpendicular to the ridge causes overlying tissues to buckle. In this case, mucopolysaccharide-rich connective tissue provides structural support. These six species possess changeable papillae that are diverse in size and shape, yet with one exception they share somewhat similar functional morphologies. Future research on papilla morphology, biomechanics and neural control in the many unexamined species of octopus and cuttlefish may uncover new principles of actuation in soft, flexible tissue.

  3. Quantitative Morphologic Analysis of Boulder Shape and Surface Texture to Infer Environmental History: A Case Study of Rock Breakdown at the Ephrata Fan, Channeled Scabland, Washington

    NASA Technical Reports Server (NTRS)

    Ehlmann, Bethany L.; Viles, Heather A.; Bourke, Mary C.

    2008-01-01

    Boulder morphology reflects both lithology and climate and is dictated by the combined effects of erosion, transport, and weathering. At present, morphologic information at the boulder scale is underutilized as a recorder of environmental processes, partly because of the lack of a systematic quantitative parameter set for reporting and comparing data sets. We develop such a parameter set, incorporating a range of measures of boulder form and surface texture. We use standard shape metrics measured in the field and fractal and morphometric classification methods borrowed from landscape analysis and applied to laser-scanned molds. The parameter set was pilot tested on three populations of basalt boulders with distinct breakdown histories in the Channeled Scabland, Washington: (1) basalt outcrop talus; (2) flood-transported boulders recently excavated from a quarry; and (3) flood-transported boulders, extensively weathered in situ on the Ephrata Fan surface. Size and shape data were found to distinguish between flood-transported and untransported boulders. Size and edge angles (approximately 120 degrees) of flood-transported boulders suggest removal by preferential fracturing along preexisting columnar joints, and curvature data indicate rounding relative to outcrop boulders. Surface textural data show that boulders which have been exposed at the surface are significantly rougher than those buried by fan sediments. Past signatures diagnostic of flood transport still persist on surface boulders, despite ongoing overprinting by processes in the present breakdown environment through roughening and fracturing in situ. Further use of this quantitative boulder parameter set at other terrestrial and planetary sites will aid in cataloging and understanding morphologic signatures of environmental processes.

  4. The comparative osteology of the petrotympanic complex (ear region) of extant baleen whales (Cetacea: Mysticeti).

    PubMed

    Ekdale, Eric G; Berta, Annalisa; Deméré, Thomas A

    2011-01-01

    Anatomical comparisons of the ear region of baleen whales (Mysticeti) are provided through detailed osteological descriptions and high-resolution photographs of the petrotympanic complex (tympanic bulla and petrosal bone) of all extant species of mysticete cetaceans. Salient morphological features are illustrated and identified, including overall shape of the bulla, size of the conical process of the bulla, morphology of the promontorium, and the size and shape of the anterior process of the petrosal. We place our comparative osteological observations into a phylogenetic context in order to initiate an exploration into petrotympanic evolution within Mysticeti. The morphology of the petrotympanic complex is diagnostic for individual species of baleen whale (e.g., sigmoid and conical processes positioned at midline of bulla in Balaenoptera musculus; confluence of fenestra cochleae and perilymphatic foramen in Eschrichtius robustus), and several mysticete clades are united by derived characteristics. Balaenids and neobalaenids share derived features of the bulla, such as a rhomboid shape and a reduced anterior lobe (swelling) in ventral aspect, and eschrichtiids share derived morphologies of the petrosal with balaenopterids, including loss of a medial promontory groove and dorsomedial elongation of the promontorium. Monophyly of Balaenoidea (Balaenidae and Neobalaenidae) and Balaenopteroidea (Balaenopteridae and Eschrichtiidae) was recovered in phylogenetic analyses utilizing data exclusively from the petrotympanic complex. This study fills a major gap in our knowledge of the complex structures of the mysticete petrotympanic complex, which is an important anatomical region for the interpretation of the evolutionary history of mammals. In addition, we introduce a novel body of phylogenetically informative characters from the ear region of mysticetes. Our detailed anatomical descriptions, illustrations, and comparisons provide valuable data for current and future studies on the phylogenetic relationships, evolution, and auditory physiology of mysticetes and other cetaceans throughout Earth's history.

  5. The effect of operating and formulation variables on the morphology of spray-dried protein particles.

    PubMed

    Maa, Y F; Costantino, H R; Nguyen, P A; Hsu, C C

    1997-08-01

    The purpose of this research was to investigate the shape and morphology of various spray-dried protein powders as a function of spray-drying conditions and protein formulations. A benchtop spray dryer was used to spray dry three model proteins in formulation with a sugar or a surfactant. Physical characterizations of the powder included morphology (scanning electron microscopy), particle size, residual moisture, and X-ray powder diffraction analyses. A significant change in particle shape from irregular (e.g., "donut") to spherical was observed as the outlet temperature of the dryer was decreased. The drying air outlet temperature was shown to depend on various operating parameters and was found to correlate with the drying rate of atomized droplets in the drying chamber. The morphology of spray-dried protein particles was also affected by formulation. In protein:sugar formulations, spray-dried particles exhibited a smooth surface regardless of the protein-to-lactose ratio, whereas roughness was observed when mannitol was present at > 30% of total solids, due to recrystallization. Protein particles containing trehalose at concentrations > 50% were highly agglomerated. The presence of surfactant resulted in noticeably smoother, more spherical particles. The shape and the morphology of spray-dried powders are affected by spray drying conditions and protein formulation. This study provides information useful for development of dry proteins for fine powder (e.g., aerosol) applications.

  6. Quantitative grading of a human blastocyst: optimal inner cell mass size and shape.

    PubMed

    Richter, K S; Harris, D C; Daneshmand, S T; Shapiro, B S

    2001-12-01

    To investigate the predictive value of quantitative measurements of blastocyst morphology on subsequent implantation rates after transfer. Prospective observational study. Private assisted reproductive technology center. One hundred seventy-four IVF patients receiving transfers of expanded blastocyst-stage embryos on day 5 (n = 112) or day 6 (n = 62) after oocyte retrieval. None. Blastocyst diameter, number of trophectoderm cells, inner cell mass (ICM) size, ICM shape, and implantation and pregnancy rates. Blastocyst diameter and trophectoderm cell numbers were unrelated to implantation rates. Day 5 expanded blastocysts with ICMs of >4,500 microm(2) implanted at a higher rate than did those with smaller ICMs (55% vs. 31%). Day 5 expanded blastocysts with slightly oval ICMs implanted at a higher rate (58%) compared with those with either rounder ICMs (7%) or more elongated ICMs (33%). Implantation rates were highest (71%) for embryos with both optimal ICM size and shape. Pregnancy rates were higher for day 5 transfers of optimally shaped ICMs compared with day 5 transfers of optimally sized ICMs. Quantitative measurements of the inner cell mass are highly indicative of blastocyst implantation potential. Blastocysts with relatively large and/or slightly oval ICMs are more likely to implant than other blastocysts.

  7. Ontogenetic shifts in morphology and resource use of cisco Coregonus artedi.

    PubMed

    Muir, A M; Vecsei, P; Pratt, T C; Krueger, C C; Power, M; Reist, J D

    2013-02-01

    Two previously described lacustrine cisco Coregonus spp. morphs [i.e. a small (<300 mm fork length, L(F)), low-gillraker (≤44) morph and a large (≥300 mm L(F) ), high-gillraker (≥45) morph] from Great Slave Lake, NT, Canada, were found to be synonymous with cisco Coregonus artedi. Geometric body shape did not differ between the two size classes nor could they be differentiated by 24 size-corrected linear measurements, indicating that the two groups had similar phenotypes. Strong, positive correlations between all linear characters and geometric centroid size (a composite variable of fish body length, mass and age) suggested that body morphology changed with age as fish grew. Total gillraker number (N(GR)) increased with L(F) according to: N(GR) = 36.3 + 0.034L(F). Differences in gillraker number and phenotype with age and size were explained by shifts in habitat and trophic resource use. Relative abundance within 0-30, 30-60, 60-90 and >90 m depth strata differed between size classes suggesting that morphology changed when fish shifted their habitat as they grew older. Large C. artedi had lower δ(13)C and slightly higher δ(15)N, indicating greater reliance on pelagic prey resources (i.e. more or larger zooplankton, such as Mysis spp.), compared to small C. artedi, which relied slightly more on benthic prey. Gillraker shape and number have always been used as key diagnostic characters in coregonine taxonomy; based on the findings presented here, ontogenetic shifts should be accounted for in resulting classifications. © 2013 The Authors. Journal of Fish Biology © 2013 The Fisheries Society of the British Isles.

  8. Nano-sized ZnO powders prepared by co-precipitation method with various pH

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Purwaningsih, S. Y., E-mail: sriyanisaputri@gmail.com; Pratapa, S.; Triwikantoro,

    2016-04-19

    In this work, nano-sized ZnO powders have been synthesized by the co-precipitation method with Zn(CH3COOH)2.2H2O, HCl, and NH3.H2O as raw materials in various pH ranging from 8 to 10. The purity, microstructure, chemical group analysis, morphology of the prepared ZnO powders were studied by X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), energy dispersive X-ray spectrometry (EDX), and scanning electron microscope (SEM), respectively. Rietveld refinement of XRD data showed that ZnO crystallizes in the wurtzite structure with high purity. The obtained powders were nano-sized particles with the average crystallite size about 17.9 ± 2.1 nm synthesized with pH of 9.5, atmore » 85°C, and stirring time of 6 h. The SEM results have visualied the morphology of ZnO nanoparticles with spherical-like shape. The effect of processing conditions on morphology of ZnO was also discussed.« less

  9. Hydrothermal synthesis of zinc oxide nanoparticles using rice as soft biotemplate.

    PubMed

    Ramimoghadam, Donya; Bin Hussein, Mohd Zobir; Taufiq-Yap, Yun Hin

    2013-01-01

    Rice as a renewable, abundant bio-resource with unique characteristics can be used as a bio-template to synthesize various functional nanomaterials. Therefore, the effect of uncooked rice flour as bio-template on physico-chemical properties, especially the morphology of zinc oxide nanostructures was investigated in this study. The ZnO particles were synthesized through hydrothermal-biotemplate method using zinc acetate-sodium hydroxide and uncooked rice flour at various ratios as precursors at 120°C for 18 hours. The results indicate that rice as a bio-template can be used to modify the shape and size of zinc oxide particles. Different morphologies, namely flake-, flower-, rose-, star- and rod-like structures were obtained with particle size at micro- and nanometer range. Pore size and texture of the resulting zinc oxide particles were found to be template-dependent and the resulting specific surface area enhanced compared to the zinc oxide synthesized without rice under the same conditions. However, optical property particularly the band gap energy is generally quite similar. Pure zinc oxide crystals were successfully synthesized using rice flour as biotemplate at various ratios of zinc salt to rice. The size- and shape-controlled capability of rice to assemble the ZnO particles can be employed for further useful practical applications.

  10. Shape Variation in Aterian Tanged Tools and the Origins of Projectile Technology: A Morphometric Perspective on Stone Tool Function

    PubMed Central

    Iovita, Radu

    2011-01-01

    Background Recent findings suggest that the North African Middle Stone Age technocomplex known as the Aterian is both much older than previously assumed, and certainly associated with fossils exhibiting anatomically modern human morphology and behavior. The Aterian is defined by the presence of ‘tanged’ or ‘stemmed’ tools, which have been widely assumed to be among the earliest projectile weapon tips. The present study systematically investigates morphological variation in a large sample of Aterian tools to test the hypothesis that these tools were hafted and/or used as projectile weapons. Methodology/Principal Findings Both classical morphometrics and Elliptical Fourier Analysis of tool outlines are used to show that the shape variation in the sample exhibits size-dependent patterns consistent with a reduction of the tools from the tip down, with the tang remaining intact. Additionally, the process of reduction led to increasing side-to-side asymmetries as the tools got smaller. Finally, a comparison of shape-change trajectories between Aterian tools and Late Paleolithic arrowheads from the North German site of Stellmoor reveal significant differences in terms of the amount and location of the variation. Conclusions/Significance The patterns of size-dependent shape variation strongly support the functional hypothesis of Aterian tools as hafted knives or scrapers with alternating active edges, rather than as weapon tips. Nevertheless, the same morphological patterns are interpreted as one of the earliest evidences for a hafting modification, and for the successful combination of different raw materials (haft and stone tip) into one implement, in itself an important achievement in the evolution of hominin technologies. PMID:22216161

  11. Facile controlled synthesis of micro/nanostructure MCrO 4 (M = Ba, Pb) by using Gemini surfactant C 12-PEG-C 12 as a soft template

    NASA Astrophysics Data System (ADS)

    Chang, Wengui; Shen, Yuhua; Xie, Anjian; Liu, Xue

    2010-04-01

    Gemini surfactants, double sodium α-sulfonic polyethylene glycol laurate (abbreviated C 12-PEG-C 12), were prepared and applied as soft templates in the controlled synthesis of BaCrO 4 and PbCrO 4 micro/nanocrystals. The template effects were investigated by adjusting the length of the spacer, using PEG400 and PEG4000, of the Gemini surfactant. The results indicated that the size and morphology of BaCrO 4 and PbCrO 4 micro/nanocrystals varied with the change in spacer length of C 12-PEG-C 12, suggesting that the different lengths of the polyethylene glycol group spacers in the Gemini surfactants played a key role in determining the size and shape of the MCrO 4 micro/nanoparticles. The dynamic process of the formation of the novel morphology BaCrO 4 crystals showed that the morphology grew from a round-bar polyhedron, to regular polyhedron, to approximate octahedron to a uniform pistachio nut shape. The growth mechanism of the BaCrO 4 micro/nanocrystals was explained that C 12-PEG-C 12 had a greater interfacial adsorption and would effectively control the shape evolution during the crystal growth, while PbCrO 4 could be explained that the Gemini surfactants can undergo liquid-crystalline phase transitions with long channels providing a soft template effect and derived the nanorods formation. Room temperature fluorescence spectra were studied and these showed that the pistachio-shaped BaCrO 4 microcrystals and PbCrO 4 nanorods possess photoactive luminescence properties with emission peaks at 470 and 549 nm, respectively.

  12. Hemodynamic and morphological characteristics of unruptured posterior communicating artery aneurysms with oculomotor nerve palsy.

    PubMed

    Lv, Nan; Yu, Ying; Xu, Jinyu; Karmonik, Christof; Liu, Jianmin; Huang, Qinghai

    2016-08-01

    OBJECT Unruptured posterior communicating artery (PCoA) aneurysms with oculomotor nerve palsy (ONP) have a very high risk of rupture. This study investigated the hemodynamic and morphological characteristics of intracranial aneurysms with high rupture risk by analyzing PCoA aneurysms with ONP. METHODS Fourteen unruptured PCoA aneurysms with ONP, 33 ruptured PCoA aneurysms, and 21 asymptomatic unruptured PCoA aneurysms were included in this study. The clinical, morphological, and hemodynamic characteristics were compared among the different groups. RESULTS The clinical characteristics did not differ among the 3 groups (p > 0.05), whereas the morphological and hemodynamic analyses showed that size, aspect ratio, size ratio, undulation index, nonsphericity index, ellipticity index, normalized wall shear stress (WSS), and percentage of low WSS area differed significantly (p < 0.05) among the 3 groups. Furthermore, multiple comparisons revealed that these parameters differed significantly between the ONP group and the asymptomatic unruptured group and between the ruptured group and the asymptomatic unruptured group, except for size, which differed significantly only between the ONP group and the asymptomatic unruptured group (p = 0.0005). No morphological or hemodynamic parameters differed between the ONP group and the ruptured group. CONCLUSIONS Unruptured PCoA aneurysms with ONP demonstrated a distinctive morphological-hemodynamic pattern that was significantly different compared with asymptomatic unruptured PCoA aneurysms and was similar to ruptured PCoA aneurysms. The larger size, more irregular shape, and lower WSS might be related to the high rupture risk of PCoA aneurysms.

  13. In-depth understanding of the relation between CuAlO₂ particle size and morphology for ozone gas sensor detection at a nanoscale level.

    PubMed

    Thirumalairajan, S; Mastelaro, Valmor R; Escanhoela, Carlos A

    2014-12-10

    A morphology-dependent nanomaterial for energy and environment applications is one of the key challenges for materials science and technology. In this study, we investigate the effect of the particle size of CuAlO2 nanostructures prepared through the facile and hydrothermal process to detect ozone gas. Phase analysis and structural information were obtained using X-ray diffraction and micro-Raman studies. The chemical states of CuAlO2 atomic species were determined by X-ray photoelectron spectroscopy. Electron microscopy images revealed the flower and hexagonal shape constituted of pentagon and oval CuAlO2 nanoparticles with average size ∼40 and 80 nm. The specific surface area was measured and found to be 59.8 and 70.8 m(2) g(-1), respectively. The developed CuAlO2 nanostructures not only possess unique morphology but also influence the ozone gas sensing performance. Among the two structures, CuAlO2, with hexagonal morphology, exhibited superior ozone detection for 200 ppb at 250 °C, with a response and good recovery time of 25 and 39 s compared to the flower morphology (28 and 69 s). These results show that not only does the morphology play an major role but also the particle size, surface area, gas adsorption/desorption, and grain-grain contact, as proposed in the gas sensing mechanism. Finally, we consider CuAlO2 material as a good candidate for environment monitoring applications.

  14. Controlling the Morphology and Oxidation Resistance of Boron Carbide Synthesized Via Carbothermic Reduction Reaction

    NASA Astrophysics Data System (ADS)

    Ahmed, Yasser M. Z.; El-Sheikh, Said M.; Ewais, Emad M. M.; Abd-Allah, Asmaa A.; Sayed, Said A.

    2017-03-01

    Boron carbide powder was synthesized from boric acid and lactose mixtures via easy procedure. Boric acid and lactose solution mixtures were roasted in stainless steel pot at 280 °C for 24 h. Boron carbide was obtained by heating the roasted samples under flowing of industrial argon gas at 1500 °C for 3 h. The amount of borate ester compound in the roasted samples was highly influenced by the boron/carbon ratio in the starting mixtures and plays a versatile role in the produced boron carbide. The high-purity boron carbide powder was produced with a sample composed of lowest boron/carbon ratio of 1:1 without calcination step. Particle morphology was changed from nano-needles like structure of 8-10 nm size with highest carbon ratio mixture to spherical shape of >150 nm size with lowest one. The oxidation resistance performance of boron carbide is highly dependent on the morphology and grain size of the synthesized powder.

  15. Galaxy evolution by color-log(n) type since redshift unity in the Hubble Ultra Deep Field

    NASA Astrophysics Data System (ADS)

    Cameron, E.; Driver, S. P.

    2009-01-01

    Aims: We explore the use of the color-log(n) (where n is the global Sérsic index) plane as a tool for subdividing the galaxy population in a physically-motivated manner out to redshift unity. We thereby aim to quantify surface brightness evolution by color-log(n) type, accounting separately for the specific selection and measurement biases against each. Methods: We construct (u-r) color-log(n) diagrams for distant galaxies in the Hubble Ultra Deep Field (UDF) within a series of volume-limited samples to z=1.5. The color-log(n) distributions of these high redshift galaxies are compared against that measured for nearby galaxies in the Millennium Galaxy Catalogue (MGC), as well as to the results of visual morphological classification. Based on this analysis we divide our sample into three color-structure classes. Namely, “red, compact”, “blue, diffuse” and “blue, compact”. Luminosity-size diagrams are constructed for members of the two largest classes (“red, compact” and “blue, diffuse”), both in the UDF and the MGC. Artificial galaxy simulations (for systems with exponential and de Vaucouleurs profile shapes alternately) are used to identify “bias-free” regions of the luminosity-size plane in which galaxies are detected with high completeness, and their fluxes and sizes recovered with minimal surface brightness-dependent biases. Galaxy evolution is quantified via comparison of the low and high redshift luminosity-size relations within these “bias-free” regions. Results: We confirm the correlation between color-log(n) plane position and visual morphological type observed locally and in other high redshift studies in the color and/or structure domain. The combined effects of observational uncertainties, the morphological K-correction and cosmic variance preclude a robust statistical comparison of the shape of the MGC and UDF color-log(n) distributions. However, in the interval 0.75 < z <1.0 where the UDF i-band samples close to rest-frame B-band light (i.e., the morphological K-correction between our samples is negligible) we are able to present tentative evidence of bimodality, albiet for a very small sample size (17 galaxies). Our unique approach to quantifying selection and measurement biases in the luminosity-size plane highlights the need to consider errors in the recovery of both magnitudes and sizes, and their dependence on profile shape. Motivated by these results we divide our sample into the three color-structure classes mentioned above and quantify luminosity-size evolution by galaxy type. Specifically, we detect decreases in B-band, surface brightness of 1.57 ± 0.22 mag arcsec-2 and 1.65 ± 0.22 mag arcsec-2 for our “blue, diffuse” and “red, compact” classes respectively between redshift unity and the present day.

  16. Variation in tooth morphology of Gorilla gorilla.

    PubMed

    Uchida, A

    1998-01-01

    Gorilla gorilla exemplifies a species that shows considerable variation in habitat, behaviour, genetic structure and morphology. This study examines variation of dental morphology in gorillas. Despite the marked size dimorphism, there are no significant shape differences between the sexes within subspecies. Differences in dental morphology, including tooth cusp proportions between the western G. g. gorilla and the eastern G. g. beringei are considerable. Although more similar to G. g. beringei than to the western G. g. gorilla, G. g. graueri also shows distinct morphological features. This indicates that the morphology of G. g. graueri is not merely intermediate, and genetic isolation between the two eastern subspecies could have had a substantial influence. Such extensive variation in dental morphology in Gorilla gorilla can be considered to be the result of an interesting combination of factors, including local dietary adaptations.

  17. Ultrasound assisted crystallization of mefenamic acid: Effect of operating parameters and comparison with conventional approach.

    PubMed

    Iyer, Sneha R; Gogate, Parag R

    2017-01-01

    The current work investigates the application of low intensity ultrasonic irradiation for improving the cooling crystallization of Mefenamic Acid for the first time. The crystal shape and size has been analyzed with the help of optical microscope and image analysis software respectively. The effect of ultrasonic irradiation on crystal size, particle size distribution (PSD) and yield has been investigated, also establishing the comparison with conventional approach. It has been observed that application of ultrasound not only enhances the yield but also reduces the induction time for crystallization as compared to conventional cooling crystallization technique. In the presence of ultrasound, the maximum yield was obtained at optimum conditions of power dissipation of 30W and ultrasonic irradiation time of 10min. The yield was further improved by application of ultrasound in cycles where the formed crystals are allowed to grow in the absence of ultrasonic irradiation. It was also observed that the desired crystal morphology was obtained for the ultrasound assisted crystallization. The conventionally obtained needle shaped crystals transformed into plate shaped crystals for the ultrasound assisted crystallization. The particle size distribution was analyzed using statistical means on the basis of skewness and kurtosis values. It was observed that the skewness and excess kurtosis value for ultrasound assisted crystallization was significantly lower as compared to the conventional approach. XRD analysis also revealed better crystal properties for the processed mefenamic acid using ultrasound assisted approach. The overall process intensification benefits of mefenamic acid crystallization using the ultrasound assisted approach were reduced particle size, increase in the yield and uniform PSD coupled with desired morphology. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Solar-assisted synthesis of ZnO nanoparticles using lime juice: a green approach

    NASA Astrophysics Data System (ADS)

    Hinge, Shruti P.; Pandit, Aniruddha B.

    2017-12-01

    Zinc oxide (ZnO) nanoparticles are those nanoparticles which have been synthesized in various morphologies and shapes. Their size and shape dependent properties and their applications in vivid sectors of science and technology make them interesting to synthesize. Present work reports a green method for ZnO nanoparticle synthesis using lime juice and sunlight. ZnO nanoparticles were also synthesized by conventionally used methods like heating, stirring or no heating and/or stirring. The nanoparticles were characterized using different techniques like UV-vis spectroscopy, scanning electron microscopy (SEM), x-ray diffraction (XRD) and dynamic light scattering (DLS). Thermo gravimetric analysis (TGA) was also carried out for the intermediate product to select the calcination temperature. Stoichiometric study reveals that the intermediate product formed is zinc citrate dihydrate. The synthesized calcined nanoparticles have good crystallinity, uniform shape, and high purity and were in the size range of 20-30 nm. These nanoparticles formed agglomerates of various shapes in the size range of 200-750 nm. This process is ecofriendly and is amiable for easy scale up.

  19. Can skull form predict the shape of the temporomandibular joint? A study using geometric morphometrics on the skulls of wolves and domestic dogs.

    PubMed

    Curth, Stefan; Fischer, Martin S; Kupczik, Kornelius

    2017-11-01

    The temporomandibular joint (TMJ) conducts and restrains masticatory movements between the mammalian cranium and the mandible. Through this functional integration, TMJ morphology in wild mammals is strongly correlated with diet, resulting in a wide range of TMJ variations. However, in artificially selected and closely related domestic dogs, dietary specialisations between breeds can be ruled out as a diversifying factor although they display an enormous variation in TMJ morphology. This raises the question of the origin of this variation. Here we hypothesise that, even in the face of reduced functional demands, TMJ shape in dogs can be predicted by skull form; i.e. that the TMJ is still highly integrated in the dog skull. If true, TMJ variation in the dog would be a plain by-product of the enormous cranial variation in dogs and its genetic causes. We addressed this hypothesis using geometric morphometry on a data set of 214 dog and 60 wolf skulls. We digitized 53 three-dimensional landmarks of the skull and the TMJ on CT-based segmentations and compared (1) the variation between domestic dog and wolf TMJs (via principal component analysis) and (2) the pattern of covariation of skull size, flexion and rostrum length with TMJ shape (via regression of centroid size on shape and partial least squares analyses). We show that the TMJ in domestic dogs is significantly more diverse than in wolves: its shape covaries significantly with skull size, flexion and rostrum proportions in patterns which resemble those observed in primates. Similar patterns in canids, which are carnivorous, and primates, which are mostly frugivorous imply the existence of basic TMJ integration patterns which are independent of dietary adaptations. However, only limited amounts of TMJ variation in dogs can be explained by simple covariation with overall skull geometry. This implies that the final TMJ shape is gained partially independently of the rest of the skull. Copyright © 2017 Elsevier GmbH. All rights reserved.

  20. Effect of carbon source on the morphology and electrochemical performances of LiFePO4/C nanocomposites.

    PubMed

    Liu, Shuxin; Wang, Haibin; Yin, Hengbo; Wang, Hong; He, Jichuan

    2014-03-01

    The carbon coated LiFePO4 (LiFePO4/C) nanocomposites materials were successfully synthesized by sol-gel method. The microstructure and morphology of LiFePO4/C nanocomposites were characterized by X-ray diffraction, Raman spectroscopy and scanning electron microscopy. The results showed that the carbon layers decomposed by different dispersant and carbon source had different graphitization degree, and the sugar could decompose to form more graphite-like structure carbon. The carbon source and heat-treatment temperature had some effect on the particle size and morphology, the sample LFP-S700 synthesized by adding sugar as carbon source at 700 degrees C had smaller particle size, uniform size distribution and spherical shape. The electrochemical behavior of LiFePO4/C nanocomposites was analyzed using galvanostatic measurements and cyclic voltammetry (CV). The results showed that the sample LFP-S700 had higher discharge specific capacities, higher apparent lithium ion diffusion coefficient and lower charge transfer resistance. The excellent electrochemical performance of sample LFP-S700 could be attributed to its high graphitization degree of carbon, smaller particle size and uniform size distribution.

  1. Characterization of Particle Size Standard NIST 1019b with SynchrotronX-ray Microtomography and Digital Data Extraction

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Friedrich, Jon M.; Rivers, Mark L.; Perlowitz, Michael A.

    We show that synchrotron x-ray microtomography ({mu}CT) followed by digital data extraction can be used to examine the size distribution and particle morphologies of the polydisperse (750 to 2450 {micro}m diameter) particle size standard NIST 1019b. Our size distribution results are within errors of certified values with data collected at 19.5 {micro}m/voxel. One of the advantages of using {mu}CT to investigate the particles examined here is that the morphology of the glass beads can be directly examined. We use the shape metrics aspect ratio and sphericity to examine of individual standard beads morphologies as a function of spherical equivalent diameters.more » We find that the majority of standard beads possess near-spherical aspect ratios and sphericities, but deviations are present at the lower end of the size range. The majority (> 98%) of particles also possess an equant form when examined using a common measure of equidimensionality. Although the NIST 1019b standard consists of loose particles, we point out that an advantage of {mu}CT is that coherent materials comprised of particles can be examined without disaggregation.« less

  2. Size Variation under Domestication: Conservatism in the inner ear shape of wolves, dogs and dingoes.

    PubMed

    Schweizer, Anita V; Lebrun, Renaud; Wilson, Laura A B; Costeur, Loïc; Schmelzle, Thomas; Sánchez-Villagra, Marcelo R

    2017-10-17

    A broad sample of wolves, dingoes, and domesticated dogs of different kinds and time periods was used to identify changes in size and shape of the organs of balance and hearing related to domestication and to evaluate the potential utility of uncovered patterns as markers of domestication. Using geometric morphometrics coupled with non-invasive imaging and three-dimensional reconstructions, we exposed and compared complex structures that remain largely conserved. There is no statistically significant difference in the levels of shape variation between prehistoric and modern dogs. Shape variance is slightly higher for the different components of the inner ear in modern dogs than in wolves, but these differences are not significant. Wolves express a significantly greater level of variance in the angle between the lateral and the posterior canal than domestic dog breeds. Wolves have smaller levels of size variation than dogs. In terms of the shape of the semicircular canals, dingoes reflect the mean shape in the context of variation in the sample. This mirrors the condition of feral forms in other organs, in which there is an incomplete return to the characteristics of the ancestor. In general, morphological diversity or disparity in the inner ear is generated by scaling.

  3. Facile and green synthesis of mesoporous Co3O4 nanocubes and their applications for supercapacitors

    NASA Astrophysics Data System (ADS)

    Liu, Xiangmei; Long, Qing; Jiang, Chunhui; Zhan, Beibei; Li, Chen; Liu, Shujuan; Zhao, Qiang; Huang, Wei; Dong, Xiaochen

    2013-06-01

    Nanostructured Co3O4 materials attracted significant attention due to their exceptional electrochemical (pseudo-capacitive) properties. However, rigorous preparation conditions are needed to control the size (especially nanosize), morphology and size distribution of the products obtained by conventional methods. Herein, we describe a novel one step shape-controlled synthesis of uniform Co3O4 nanocubes with a size of 50 nm with the existence of mesoporous carbon nanorods (meso-CNRs). In this synthesis process, meso-CNRs not only act as a heat receiver to directly obtain Co3O4 eliminating the high-temperature post-calcination, but also control the morphology of the resulting Co3O4 to form nanocubes with uniform distribution. More strikingly, mesoporous Co3O4 nanocubes are obtained by further thermal treatment. The structure and morphology of the samples were characterized by scanning electron microscopy, transmission electron microscopy and X-ray diffraction. A possible formation mechanism of mesoporous Co3O4 nanocubes is proposed here. Electrochemical tests have revealed that the prepared mesoporous Co3O4 nanocubes demonstrate a remarkable performance in supercapacitor applications due to the porous structure, which endows fast ion and electron transfer.Nanostructured Co3O4 materials attracted significant attention due to their exceptional electrochemical (pseudo-capacitive) properties. However, rigorous preparation conditions are needed to control the size (especially nanosize), morphology and size distribution of the products obtained by conventional methods. Herein, we describe a novel one step shape-controlled synthesis of uniform Co3O4 nanocubes with a size of 50 nm with the existence of mesoporous carbon nanorods (meso-CNRs). In this synthesis process, meso-CNRs not only act as a heat receiver to directly obtain Co3O4 eliminating the high-temperature post-calcination, but also control the morphology of the resulting Co3O4 to form nanocubes with uniform distribution. More strikingly, mesoporous Co3O4 nanocubes are obtained by further thermal treatment. The structure and morphology of the samples were characterized by scanning electron microscopy, transmission electron microscopy and X-ray diffraction. A possible formation mechanism of mesoporous Co3O4 nanocubes is proposed here. Electrochemical tests have revealed that the prepared mesoporous Co3O4 nanocubes demonstrate a remarkable performance in supercapacitor applications due to the porous structure, which endows fast ion and electron transfer. Electronic supplementary information (ESI) available. See DOI: 10.1039/c3nr00495c

  4. Organic-inorganic hybrid mesoporous silicas: functionalization, pore size, and morphology control.

    PubMed

    Park, Sung Soo; Ha, Chang-Sik

    2006-01-01

    Topological design of mesoporous silica materials, pore architecture, pore size, and morphology are currently major issues in areas such as catalytic conversion of bulky molecules, adsorption, host-guest chemistry, etc. In this sense, we discuss the pore size-controlled mesostructure, framework functionalization, and morphology control of organic-inorganic hybrid mesoporous silicas by which we can improve the applicability of mesoporous materials. First, we explain that the sizes of hexagonal- and cubic-type pores in organic-inorganic hybrid mesoporous silicas are well controlled from 24.3 to 98.0 A by the direct micelle-control method using an organosilica precursor and surfactants with different alkyl chain lengths or triblock copolymers as templates and swelling agents incorporated in the formed micelles. Second, we describe that organic-inorganic hybrid mesoporous materials with various functional groups form various external morphologies such as rod, cauliflower, film, rope, spheroid, monolith, and fiber shapes. Third, we discuss that transition metals (Ti and Ru) and rare-earth ions (Eu(3+) and Tb(3+)) are used to modify organic-inorganic hybrid mesoporous silica materials. Such hybrid mesoporous silica materials are expected to be applied as excellent catalysts for organic reactions, photocatalysis, optical devices, etc. c) 2006 The Japan Chemical Journal Forum and Wiley Periodicals, Inc.

  5. PMMA Third-Body Wear after Unicondylar Knee Arthroplasty Decuples the UHMWPE Wear Particle Generation In Vitro

    PubMed Central

    Paulus, Alexander Christoph; Franke, Manja; Kraxenberger, Michael; Schröder, Christian; Jansson, Volkmar

    2015-01-01

    Introduction. Overlooked polymethylmethacrylate after unicondylar knee arthroplasty can be a potential problem, since this might influence the generated wear particle size and morphology. The aim of this study was the analysis of polyethylene wear in a knee wear simulator for changes in size, morphology, and particle number after the addition of third-bodies. Material and Methods. Fixed bearing unicondylar knee prostheses (UKA) were tested in a knee simulator for 5.0 million cycles. Following bone particles were added for 1.5 million cycles, followed by 1.5 million cycles with PMMA particles. A particle analysis by scanning electron microscopy of the lubricant after the cycles was performed. Size and morphology of the generated wear were characterized. Further, the number of particles per 1 million cycles was calculated for each group. Results. The particles of all groups were similar in size and shape. The number of particles in the PMMA group showed 10-fold higher values than in the bone and control group (PMMA: 10.251 × 1012; bone: 1.145 × 1012; control: 1.804 × 1012). Conclusion. The addition of bone or PMMA particles in terms of a third-body wear results in no change of particle size and morphology. PMMA third-bodies generated tenfold elevated particle numbers. This could favor an early aseptic loosening. PMID:25866795

  6. A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed.

    PubMed

    Zhao, Lingxiao; Pan, Ting; Guo, Dongwei; Wei, Cunxu

    2018-01-01

    Storage starch in starchy seed influences the seed weight and texture, and determines its applications in food and nonfood industries. Starch granules from different plant sources have significantly different shapes and sizes, and even more the difference exists in the different regions of the same tissue. Therefore, it is very important to in situ investigate the morphology and distribution of starch in the whole seed. However, a simple and rapid method is deficient to prepare the whole section of starchy seed for investigating the morphology and distribution of starch in the whole seeds for a large number of samples. A simple and rapid method was established to prepare the whole section of starchy seed, especially for floury seed, in this study. The whole seeds of translucent and chalky rice, vitreous and floury maize, and normal barley and wheat were sectioned successfully using the newly established method. The iodine-stained section clearly exhibited the shapes and size of starch granules in different regions of seed. The starch granules with different morphologies and iodine-staining colors existed regionally in the seeds of high-amylose rice and maize. The sections of lotus and kidney bean seeds also showed the feasibility of this method for starchy non-cereal seeds. The simple and rapid method was proven effective for preparing the whole sections of starchy seeds. The whole section of seed could be used to investigate the morphology and distribution of starch granules in different regions of the whole seed. The method was especially suitable for large sample numbers to investigate the starch morphology in short time.

  7. Microbial Biosynthesis of Silver Nanoparticles in Different Culture Media.

    PubMed

    Luo, Ke; Jung, Samuel; Park, Kyu-Hwan; Kim, Young-Rok

    2018-01-31

    Microbial biosynthesis of metal nanoparticles has been extensively studied for the applications in biomedical sciences and engineering. However, the mechanism for their synthesis through microorganism is not completely understood. In this study, several culture media were investigated for their roles in the microbial biosynthesis of silver nanoparticles (AgNPs). The size and morphology of the synthesized AgNPs were analyzed by UV-vis spectroscopy, Fourier-transform-infrared (FT-IR), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The results demonstrated that nutrient broth (NB) and Mueller-Hinton broth (MHB) among tested media effectively reduced silver ions to form AgNPs with different particle size and shape. Although the involved microorganism enhanced the reduction of silver ions, the size and shape of the particles were shown to mainly depend on the culture media. Our findings suggest that the growth media of bacterial culture play an important role in the synthesis of metallic nanoparticles with regard to their size and shape. We believe our findings would provide useful information for further exploration of microbial biosynthesis of AgNPs and their biomedical applications.

  8. Preparation and characterization of chain-like and peanut-like Fe3O4@SiO2 core-shell structure.

    PubMed

    Shi, Haowei; Huang, Yan; Cheng, Chao; Ji, Guoyuan; Yang, Yuxiang; Yuan, Hongming

    2013-10-01

    The size- and shape-controlled Fe3O4@SiO2 nanocomposites were successfully synthesized via the sol-gel method. The results showed that the size, shape, and property of the products were directly influenced by the amount of TEOS, and the concentration of water-based magnetic fluid in the coating process. The morphology and properties of the products were characterized by TEM, SEM, X-ray powder diffraction, IR and EDS. The Fe3O4@SiO2 composites with easily-controlled size arranged from 58 to 835 nm could be synthesized by adjusting the experimental parameters. When TEOS amount is 1 mL and the concentration of magnetic fluid were 30.0 and 10.0 mg/mL respectively, chain-like and peanuts-like well-dispersed Fe3O4@SiO2 particles with clear core-shell structure were obtained. These size- and shape-controlled Fe3O4@SiO2 composites may have potential application in the field of targeted drug delivery and MRI contrast agent.

  9. Automated Quantification and Integrative Analysis of 2D and 3D Mitochondrial Shape and Network Properties

    PubMed Central

    Nikolaisen, Julie; Nilsson, Linn I. H.; Pettersen, Ina K. N.; Willems, Peter H. G. M.; Lorens, James B.; Koopman, Werner J. H.; Tronstad, Karl J.

    2014-01-01

    Mitochondrial morphology and function are coupled in healthy cells, during pathological conditions and (adaptation to) endogenous and exogenous stress. In this sense mitochondrial shape can range from small globular compartments to complex filamentous networks, even within the same cell. Understanding how mitochondrial morphological changes (i.e. “mitochondrial dynamics”) are linked to cellular (patho) physiology is currently the subject of intense study and requires detailed quantitative information. During the last decade, various computational approaches have been developed for automated 2-dimensional (2D) analysis of mitochondrial morphology and number in microscopy images. Although these strategies are well suited for analysis of adhering cells with a flat morphology they are not applicable for thicker cells, which require a three-dimensional (3D) image acquisition and analysis procedure. Here we developed and validated an automated image analysis algorithm allowing simultaneous 3D quantification of mitochondrial morphology and network properties in human endothelial cells (HUVECs). Cells expressing a mitochondria-targeted green fluorescence protein (mitoGFP) were visualized by 3D confocal microscopy and mitochondrial morphology was quantified using both the established 2D method and the new 3D strategy. We demonstrate that both analyses can be used to characterize and discriminate between various mitochondrial morphologies and network properties. However, the results from 2D and 3D analysis were not equivalent when filamentous mitochondria in normal HUVECs were compared with circular/spherical mitochondria in metabolically stressed HUVECs treated with rotenone (ROT). 2D quantification suggested that metabolic stress induced mitochondrial fragmentation and loss of biomass. In contrast, 3D analysis revealed that the mitochondrial network structure was dissolved without affecting the amount and size of the organelles. Thus, our results demonstrate that 3D imaging and quantification are crucial for proper understanding of mitochondrial shape and topology in non-flat cells. In summary, we here present an integrative method for unbiased 3D quantification of mitochondrial shape and network properties in mammalian cells. PMID:24988307

  10. Growth behavior of LiMn{sub 2}O{sub 4} particles formed by solid-state reactions in air and water vapor

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kozawa, Takahiro, E-mail: t-kozawa@jwri.osaka-u.ac.jp; Yanagisawa, Kazumichi; Murakami, Takeshi

    Morphology control of particles formed during conventional solid-state reactions without any additives is a challenging task. Here, we propose a new strategy to control the morphology of LiMn{sub 2}O{sub 4} particles based on water vapor-induced growth of particles during solid-state reactions. We have investigated the synthesis and microstructural evolution of LiMn{sub 2}O{sub 4} particles in air and water vapor atmospheres as model reactions; LiMn{sub 2}O{sub 4} is used as a low-cost cathode material for lithium-ion batteries. By using spherical MnCO{sub 3} precursor impregnated with LiOH, LiMn{sub 2}O{sub 4} spheres with a hollow structure were obtained in air, while angulated particlesmore » with micrometer sizes were formed in water vapor. The pore structure of the particles synthesized in water vapor was found to be affected at temperatures below 700 °C. We also show that the solid-state reaction in water vapor is a simple and valuable method for the large-scale production of particles, where the shape, size, and microstructure can be controlled. - Graphical abstract: This study has demonstrated a new strategy towards achieving morphology control without the use of additives during conventional solid-state reactions by exploiting water vapor-induced particle growth. - Highlights: • A new strategy to control the morphology of LiMn{sub 2}O{sub 4} particles is proposed. • Water vapor-induced particle growth is exploited in solid-state reactions. • The microstructural evolution of LiMn{sub 2}O{sub 4} particles is investigated. • The shape, size and microstructure can be controlled by solid-state reactions.« less

  11. Synthesis of Zinc Oxide Nanoparticles using Anthocyanin as a Capping Agent

    NASA Astrophysics Data System (ADS)

    Septiani, N. L. W.; Yuliarto, B.; Iqbal, M.; Nugraha

    2017-05-01

    Zinc Oxide nanoparticles have been successfully synthesized by utilizing anthocyanin as a capping agent by thermal decomposition of precursor route. The influence of the high and low concentrations of the anthocyanin to the shape and size of ZnO was investigated in this work. The anthocyanin was obtained from Indonesia black rice extract with methanol as a solvent. The crystallinity and morphology properties were characterized by X-Ray Diffractometer (XRD), and Scanning Electron Microscope (SEM), respectively. XRD result showed that ZnO was formed with good crystallinity without any second phase and had a hexagonal wurtzite crystal structure. SEM result revealed that ZnO with a low concentration of anthocyanin has a spherical shape with a uniform size of about 16 nm while ZnO with a high concentration of anthocyanin has a rod-like shape. The size of spherical ZnO in this work is smaller than ZnO from the same method of synthesis without anthocyanin (~30 nm).

  12. Investigation of the effect of aggregates' morphology on concrete creep properties by numerical simulations

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lavergne, F.; Sab, K., E-mail: karam.sab@enpc.fr; Sanahuja, J.

    2015-05-15

    Prestress losses due to creep of concrete is a matter of interest for long-term operations of nuclear power plants containment buildings. Experimental studies by Granger (1995) have shown that concretes with similar formulations have different creep behaviors. The aim of this paper is to numerically investigate the effect of size distribution and shape of elastic inclusions on the long-term creep of concrete. Several microstructures with prescribed size distribution and spherical or polyhedral shape of inclusions are generated. By using the 3D numerical homogenization procedure for viscoelastic microstructures proposed by Šmilauer and Bažant (2010), it is shown that the size distributionmore » and shape of inclusions have no measurable influence on the overall creep behavior. Moreover, a mean-field estimate provides close predictions. An Interfacial Transition Zone was introduced according to the model of Nadeau (2003). It is shown that this feature of concrete's microstructure can explain differences between creep behaviors.« less

  13. The remarkable convergence of skull shape in crocodilians and toothed whales

    PubMed Central

    Evans, Alistair R.; Fitzgerald, Erich M. G.; Adams, Justin W.; Clausen, Philip D.; McHenry, Colin R.

    2017-01-01

    The striking resemblance of long-snouted aquatic mammals and reptiles has long been considered an example of morphological convergence, yet the true cause of this similarity remains untested. We addressed this deficit through three-dimensional morphometric analysis of the full diversity of crocodilian and toothed whale (Odontoceti) skull shapes. Our focus on biomechanically important aspects of shape allowed us to overcome difficulties involved in comparing mammals and reptiles, which have fundamental differences in the number and position of skull bones. We examined whether diet, habitat and prey size correlated with skull shape using phylogenetically informed statistical procedures. Crocodilians and toothed whales have a similar range of skull shapes, varying from extremely short and broad to extremely elongate. This spectrum of shapes represented more of the total variation in our dataset than between phylogenetic groups. The most elongate species (river dolphins and gharials) are extremely convergent in skull shape, clustering outside of the range of the other taxa. Our results suggest the remarkable convergence between long-snouted river dolphins and gharials is driven by diet rather than physical factors intrinsic to riverine environments. Despite diverging approximately 288 million years ago, crocodilians and odontocetes have evolved a remarkably similar morphological solution to feeding on similar prey. PMID:28275142

  14. The remarkable convergence of skull shape in crocodilians and toothed whales.

    PubMed

    McCurry, Matthew R; Evans, Alistair R; Fitzgerald, Erich M G; Adams, Justin W; Clausen, Philip D; McHenry, Colin R

    2017-03-15

    The striking resemblance of long-snouted aquatic mammals and reptiles has long been considered an example of morphological convergence, yet the true cause of this similarity remains untested. We addressed this deficit through three-dimensional morphometric analysis of the full diversity of crocodilian and toothed whale (Odontoceti) skull shapes. Our focus on biomechanically important aspects of shape allowed us to overcome difficulties involved in comparing mammals and reptiles, which have fundamental differences in the number and position of skull bones. We examined whether diet, habitat and prey size correlated with skull shape using phylogenetically informed statistical procedures. Crocodilians and toothed whales have a similar range of skull shapes, varying from extremely short and broad to extremely elongate. This spectrum of shapes represented more of the total variation in our dataset than between phylogenetic groups. The most elongate species (river dolphins and gharials) are extremely convergent in skull shape, clustering outside of the range of the other taxa. Our results suggest the remarkable convergence between long-snouted river dolphins and gharials is driven by diet rather than physical factors intrinsic to riverine environments. Despite diverging approximately 288 million years ago, crocodilians and odontocetes have evolved a remarkably similar morphological solution to feeding on similar prey. © 2017 The Author(s).

  15. Ontogenetic and static allometry in the human face: contrasting Khoisan and Inuit.

    PubMed

    Freidline, Sarah E; Gunz, Philipp; Hublin, Jean-Jacques

    2015-09-01

    Regional differences in modern human facial features are present at birth, and ontogenetic allometry contributes to variation in adults. However, details regarding differential rates of growth and timing among regional groups are lacking. We explore ontogenetic and static allometry in a cross-sectional sample spanning Africa, Europe and North America, and evaluate tempo and mode in two regional groups with very different adult facial morphology, the Khoisan and Inuit. Semilandmark geometric morphometric methods, multivariate statistics and growth simulations were used to quantify and compare patterns of facial growth and development. Regional-specific facial morphology develops early in ontogeny. The Inuit has the most distinct morphology and exhibits heterochronic differences in development compared to other regional groups. Allometric patterns differ during early postnatal development, when significant increases in size are coupled with large amounts of shape changes. All regional groups share a common adult static allometric trajectory, which can be attributed to sexual dimorphism, and the corresponding allometric shape changes resemble developmental patterns during later ontogeny. The amount and pattern of growth and development may not be shared between regional groups, indicating that a certain degree of flexibility is allowed for in order to achieve adult size. In early postnatal development the face is less constrained compared to other parts of the cranium allowing for greater evolvability. The early development of region-specific facial features combined with heterochronic differences in timing or rate of growth, reflected in differences in facial size, suggest different patterns of postnatal growth. © 2015 Wiley Periodicals, Inc.

  16. Sexual dimorphism of head morphology in three-spined stickleback Gasterosteus aculeatus.

    PubMed

    Aguirre, W E; Akinpelu, O

    2010-09-01

    This study examined sexual dimorphism of head morphology in the ecologically diverse three-spined stickleback Gasterosteus aculeatus. Male G. aculeatus had longer heads than female G. aculeatus in all 10 anadromous, stream and lake populations examined, and head length growth rates were significantly higher in males in half of the populations sampled, indicating that differences in head size increased with body size in many populations. Despite consistently larger heads in males, there was significant variation in size-adjusted head length among populations, suggesting that the relationship between head length and body length was flexible. Inter-population differences in head length were correlated between sexes, thus population-level factors influenced head length in both sexes despite the sexual dimorphism present. Head shape variation between lake and anadromous populations was greater than that between sexes. The common divergence in head shape between sexes across populations was about twice as important as the sexual dimorphism unique to each population. Finally, much of the sexual dimorphism in head length was due to divergence in the anterior region of the head, where the primary trophic structures were found. It is unclear whether the sexual dimorphism was due to natural selection for niche divergence between sexes or sexual selection. This study improves knowledge of the magnitude, growth rate divergence, inter-population variation and location of sexual dimorphism in G. aculeatus head morphology. © 2010 The Authors. Journal compilation © 2010 The Fisheries Society of the British Isles.

  17. Determination of macular hole size in relation to individual variabilities of fovea morphology.

    PubMed

    Shin, J Y; Chu, Y K; Hong, Y T; Kwon, O W; Byeon, S H

    2015-08-01

    To determine the preoperative anatomic factors in macular holes and their correlation to hole closure. Forty-six eyes with consecutive unilateral macular hole who had undergone surgery and followed up for at least 6 months were enrolled. Optical coherence tomography images and best-corrected visual acuity (BCVA) within 2 weeks prior to operation and 6 months after surgery were analyzed. The maximal hole dimension, foveal degeneration factors (inner nuclear layer cysts, outer segment (OS) shortening) and the widest foveolar floor size of the fellow eyes were measured. For overcoming preoperative individual variability of foveal morphology, an 'adjusted' hole size parameter (the ratio between the hole size and the fellow eye foveolar floor size) was used based on the fact that both eyes were morphologically symmetrical. Mean preoperative BCVA (logMAR) was 1.03±0.43 and the mean postoperative BCVA was 0.50±0.38 at 6 months. Preoperative BCVA is significantly associated with postoperative BCVA (P=0.0002). The average hole diameter was 448.9±196.8 μm and the average fellow eye foveolar floor size was 461.3±128.4 μm. There was a correlation between hole diameter and the size of the fellow eye foveolar floor (Pearson's coefficient=0.608, P<0.0001). The adjusted hole size parameter was 0.979±0.358 (0.761-2.336), which was a strong predictor for both anatomic (P=0.0281) and visual (P=0.0016) outcome. When determining the extent of preoperative hole size, we have to take into consideration the foveal morphologic variations among individuals. Hole size may be related to the original foveal shape, especially in relation to the centrifugal retraction of the foveal tissues.

  18. The female knee: anatomic variations.

    PubMed

    Conley, Sheryl; Rosenberg, Aaron; Crowninshield, Roy

    2007-01-01

    Traditional knee implants have been designed "down the middle,"based on the combined average size and shape of male and female knee anatomy.Sex-based research in the field of orthopaedics has led to new understanding of the anatomic differences between the sexes and the associated implications for women undergoing total knee arthroplasty. Through the use of a comprehensive bone morphology atlas that utilizes novel three-dimensional computed tomography analysis technology, significant anatomic differences have been documented in the shape and size of female knees compared with male knees. This research identifies three notable anatomic differences in the female population: a less prominent anterior condyle, an increased Q angle, and a reduced medial-lateral:anterior-posterior aspect ratio.

  19. Morphological and chemical analysis of bone substitutes by scanning electron microscopy and microanalysis by spectroscopy of dispersion energy.

    PubMed

    da Cruz, Gabriela Alessandra; de Toledo, Sérgio; Sallum, Enilson Antonio; de Lima, Antonio Fernando Martorelli

    2007-01-01

    This study evaluated the morphological and chemical composition of the following bone substitutes: cancellous and cortical organic bovine bone with macro and microparticle size ranging from 1.0 to 2.0 mm and 0.25 to 1.0 mm, respectively; inorganic bovine bone with particle size ranging from 0.25 to 1.0 mm; hydroxyapatite with particle size ranging from 0.75 to 1.0 mm; and demineralized freeze-dried bone allograft with particle size ranging from 0.25 to 0.5 mm. The samples were sputter-coated with gold in an ion coater, the morphology was observed and particle size was measured under vacuum by scanning electron microscopy (SEM). The chemical composition was evaluated by spectroscopy of dispersion energy (EDS) microanalysis using samples without coating. SEM analysis provided visual evidence that all examined materials have irregular shape and particle sizes larger than those informed by the manufacturer. EDS microanalysis detected the presence of sodium, calcium and phosphorus that are usual elements of the bone tissue. However, mineral elements were detected in all analyzed particles of organic bovine bone except for macro cancellous organic bovine bone. These results suggest that the examined organic bovine bone cannot be considered as a pure organic material.

  20. Molecular basis of crystal morphology-dependent adhesion behavior of mefenamic acid during tableting.

    PubMed

    Waknis, Vrushali; Chu, Elza; Schlam, Roxana; Sidorenko, Alexander; Badawy, Sherif; Yin, Shawn; Narang, Ajit S

    2014-01-01

    The molecular basis of crystal surface adhesion leading to sticking was investigated by exploring the correlation of crystal adhesion to oxidized iron coated atomic force microscope (AFM) tips and bulk powder sticking behavior during tableting of two morphologically different crystals of a model drug, mefenamic acid (MA), to differences in their surface functional group orientation and energy. MA was recrystallized into two morphologies (plates and needles) of the same crystalline form. Crystal adhesion to oxidized iron coated AFM tips and bulk powder sticking to tablet punches was assessed using a direct compression formulation. Surface functional group orientation and energies on crystal faces were modeled using Accelrys Material Studio software. Needle-shaped morphology showed higher sticking tendency than plates despite similar particle size. This correlated with higher crystal surface adhesion of needle-shaped morphology to oxidized iron coated AFM probe tips, and greater surface energy and exposure of polar functional groups. Higher surface exposure of polar functional groups correlates with higher tendency to stick to metal surfaces and AFM tips, indicating involvement of specific polar interactions in the adhesion behavior. In addition, an AFM method is identified to prospectively assess the risk of sticking during the early stages of drug development.

  1. Eyes in the sky. Interactions between asymptotic giant branch star winds and the interstellar magnetic field

    NASA Astrophysics Data System (ADS)

    van Marle, A. J.; Cox, N. L. J.; Decin, L.

    2014-10-01

    Context. The extended circumstellar envelopes (CSEs) of evolved low-mass stars display a large variety of morphologies. Understanding the various mechanisms that give rise to these extended structures is important to trace their mass-loss history. Aims: Here, we aim to examine the role of the interstellar magnetic field in shaping the extended morphologies of slow dusty winds of asymptotic giant branch (AGB) stars in an effort to pin-point the origin of so-called eye shaped CSEs of three carbon-rich AGB stars. In addition, we seek to understand if this pre-planetary nebula (PN) shaping can be responsible for asymmetries observed in PNe. Methods: Hydrodynamical simulations are used to study the effect of typical interstellar magnetic fields on the free-expanding spherical stellar winds as they sweep up the local interstellar medium (ISM). Results: The simulations show that typical Galactic interstellar magnetic fields of 5 to 10 μG are sufficient to alter the spherical expanding shells of AGB stars to appear as the characteristic eye shape revealed by far-infrared observations. The typical sizes of the simulated eyes are in accordance with the observed physical sizes. However, the eye shapes are transient in nature. Depending on the stellar and interstellar conditions, they develop after 20 000 to 200 000 yrs and last for about 50 000 to 500 000 yrs, assuming that the star is at rest relative to the local interstellar medium. Once formed, the eye shape develops lateral outflows parallel to the magnetic field. The explosion of a PN in the centre of the eye-shaped dust shell gives rise to an asymmetrical nebula with prominent inward pointing Rayleigh-Taylor instabilities. Conclusions: Interstellar magnetic fields can clearly affect the shaping of wind-ISM interaction shells. The occurrence of the eyes is most strongly influenced by stellar space motion and ISM density. Observability of this transient phase is favoured for lines-of-sight perpendicular to the interstellar magnetic field direction. The simulations indicate that shaping of the pre-PN envelope can strongly affect the shape and size of PNe. Herschel is an ESA space observatory with science instruments provided by European-led Principal Investigator consortia and with important participation from NASA.Movies are available in electronic form at http://www.aanda.org

  2. Morphology and structure of TixOy nanoparticles generated by femtosecond laser ablation in water

    NASA Astrophysics Data System (ADS)

    Donėlienė, Jolanta; Rudzikas, Matas; Rades, Steffi; Dörfel, Ilona; Peplinski, Burkhard; Sahre, Mario; Pellegrino, Francesco; Maurino, Valter; Ulbikas, Juras; Galdikas, Algirdas; Hodoroaba, Vasile-Dan

    2018-04-01

    In this work femto-second pulsed laser ablation in liquid (PLAL) procedure for the generation of titanium oxide nanoparticles (NP) is reported with the purpose of understanding morphology and structure of the newly generated NPs. Ablation duration was varied for optimization of NP generation processes between 10 and 90 min. Surface morphology of NPs as well as their size and shape (distribution) were analysed by various complementary electron microscopy techniques, i.e. SEM, TSEM and TEM. The crystalline structure of titanium oxide particles was investigated by XRD (two instruments operated in different geometries) and HR-TEM. Concentration of generated titanium oxide NPs in liquid was analysed by ICP-MS. A mix of crystalline (mainly anatase), partly crystalline and amorphous spherical titanium oxide NPs can be reported having a mean size between 10 and 20 nm, which is rather independent of the laser ablation (LA) duration. A second component consisting of irregularly shaped, but crystalline titanium oxide nanostructures is co-generated in the LA water, with more pronounced occurrence at longer LA times. The provenance of this component is assigned to those spherical particles generated in suspension and passing through the converging laser beam, being hence subject to secondary irradiation effects, e. g. fragmentation.

  3. Importance of sperm morphology during sperm transport and fertilization in mammals.

    PubMed

    García-Vázquez, Francisco A; Gadea, Joaquín; Matás, Carmen; Holt, William V

    2016-01-01

    After natural or artificial insemination, the spermatozoon starts a journey from the site of deposition to the place of fertilization. However, only a small subset of the spermatozoa deposited achieves their goal: to reach and fertilize the egg. Factors involved in controlling sperm transport and fertilization include the female reproductive tract environment, cell-cell interactions, gene expression, and phenotypic sperm traits. Some of the significant determinants of fertilization are known (i.e., motility or DNA status), but many sperm traits are still indecipherable. One example is the influence of sperm dimensions and shape upon transport within the female genital tract towards the oocyte. Biophysical associations between sperm size and motility may influence the progression of spermatozoa through the female reproductive tract, but uncertainties remain concerning how sperm morphology influences the fertilization process, and whether only the sperm dimensions per se are involved. Moreover, such explanations do not allow the possibility that the female tract is capable of distinguishing fertile spermatozoa on the basis of their morphology, as seems to be the case with biochemical, molecular, and genetic properties. This review focuses on the influence of sperm size and shape in evolution and their putative role in sperm transport and selection within the uterus and the ability to fertilize the oocyte.

  4. Importance of sperm morphology during sperm transport and fertilization in mammals

    PubMed Central

    García-Vázquez, Francisco A; Gadea, Joaquín; Matás, Carmen; Holt, William V

    2016-01-01

    After natural or artificial insemination, the spermatozoon starts a journey from the site of deposition to the place of fertilization. However, only a small subset of the spermatozoa deposited achieves their goal: to reach and fertilize the egg. Factors involved in controlling sperm transport and fertilization include the female reproductive tract environment, cell-cell interactions, gene expression, and phenotypic sperm traits. Some of the significant determinants of fertilization are known (i.e., motility or DNA status), but many sperm traits are still indecipherable. One example is the influence of sperm dimensions and shape upon transport within the female genital tract towards the oocyte. Biophysical associations between sperm size and motility may influence the progression of spermatozoa through the female reproductive tract, but uncertainties remain concerning how sperm morphology influences the fertilization process, and whether only the sperm dimensions per se are involved. Moreover, such explanations do not allow the possibility that the female tract is capable of distinguishing fertile spermatozoa on the basis of their morphology, as seems to be the case with biochemical, molecular, and genetic properties. This review focuses on the influence of sperm size and shape in evolution and their putative role in sperm transport and selection within the uterus and the ability to fertilize the oocyte. PMID:27624988

  5. Radar Investigations of Asteroids

    NASA Technical Reports Server (NTRS)

    Ostro, S. J.

    1984-01-01

    Radar investigations of asteroids, including observations during 1984 to 1985 of at least 8 potential targets and continued analyses of radar data obtained during 1980 to 1984 for 30 other asteroids is proposed. The primary scientific objectives include estimation of echo strength, polarization, spectral shape, spectral bandwidth, and Doppler shift. These measurements yield estimates of target size, shape, and spin vector; place constraints on topography, morphology, density, and composition of the planetary surface; yield refined estimates of target orbital parameters; and reveals the presence of asteroidal satellites.

  6. Shape - but Not Size - Codivergence between Male and Female Copulatory Structures in Onthophagus Beetles

    PubMed Central

    Macagno, Anna L. M.; Pizzo, Astrid; Parzer, Harald F.; Palestrini, Claudia; Rolando, Antonio; Moczek, Armin P.

    2011-01-01

    Genitalia are among the fastest evolving morphological traits in arthropods. Among the many hypotheses aimed at explaining this observation, some explicitly or implicitly predict concomitant male and female changes of genital traits that interact during copulation (i.e., lock and key, sexual conflict, cryptic female choice and pleiotropy). Testing these hypotheses requires insights into whether male and female copulatory structures that physically interact during mating also affect each other's evolution and patterns of diversification. Here we compare and contrast size and shape evolution of male and female structures that are known to interact tightly during copulation using two model systems: (a) the sister species O. taurus (1 native, 3 recently established populations) and O. illyricus, and (b) the species-complex O. fracticornis-similis-opacicollis. Partial Least Squares analyses indicated very little to no correlation between size and shape of copulatory structures, both in males and females. Accordingly, comparing shape and size diversification patterns of genitalia within each sex showed that the two components diversify readily - though largely independently of each other - within and between species. Similarly, comparing patterns of divergence across sexes showed that relative sizes of male and female copulatory organs diversify largely independent of each other. However, performing this analysis for genital shape revealed a signature of parallel divergence. Our results therefore suggest that male and female copulatory structures that are linked mechanically during copulation may diverge in concert with respect to their shapes. Furthermore, our results suggest that genital divergence in general, and co-divergence of male and female genital shape in particular, can evolve over an extraordinarily short time frame. Results are discussed in the framework of the hypotheses that assume or predict concomitant evolutionary changes in male and female copulatory organs. PMID:22194942

  7. Craniofacial structure variations in patients with palatal anomalies and velopharyngeal dysfunction.

    PubMed

    Nachmani, Ariela; Aizenbud, Dror; Nageris, Ben; Emodi, Omri; Kassem, Firas

    2017-02-01

    Cephalometric evaluation of craniofacial and craniopharyngeal morphology is important for understanding the factors affecting velopharyngeal dysfunction (VPD) in patients with palatal anomalies. In this study, 366 patients with VPD were retrospectively stratified into cleft lip and palate (CLP), cleft palate (CP), submucous cleft palate (SMCP), occult submucous cleft palate (OSMCP), and non-CP groups. Lateral cephalometrics were used to assess craniofacial, craniopharyngeal, and velopharyngeal anatomy. The average craniofacial morphology in patients with VPD differed significantly according to the type of palatal anomaly. The non-CP and OSMCP groups differed from the CLP, CP, and SMCP groups in nasopharyngeal size and shape as depicted by a larger ANS-Ptm-Ve angle, a smaller S-N-Ba and NBa-PP angles, and a shorter linear value of S-Ar in the non-CP group. The CLP and CP groups had shorter ANS-Ptm, shorter Ptm-P, and smaller SNA and SNB angles. VPD patients with overt clefts have different skeletal and nasopharyngeal shapes compared to non-CP and OSMCP. Velopharyngeal function assessment should include the size and shape of the nasopharyngeal space in addition to the size and the activity of the velum and posterior and lateral walls of the nasopharynx. This should enable a more precise understanding of VPD pathology, and lead to improvements in the posterior pharyngeal flap technique in order to obtain better postoperative speech outcomes after surgical management of velopharyngeal dysfunction. Copyright © 2016 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

  8. Dynamic morphology applied to human and animal leukemia cells.

    PubMed

    Haemmerli, G; Felix, H; Sträuli, P

    1979-08-01

    Dynamic morphology, which describes the shape and surface architecture of fixed cells in terms related to their behavior in the living state, is based on the concurrent use of two methods: scanning electron microscopy and microcinematography. This combination has both advantages and disadvantages. In this study on leukemic cells, we were able to draw the following conclusions about the usefulness of dynamic morphology. It confirms that white blood cells do not flatten on a glass substrate; they stay spherical and are either round or polarized. Round cells of similar size, whatever their origin, cannot be classified by dynamic morphology. Polarized cells can be classified as blasts, promyelocytes, myelocytes, granulocytes and lymphocytes, although polarized blast cells of different origins cannot be differentiated. Dynamic morphology cannot classify the same cell type as benign or malignant.

  9. Growth mechanics of bacterial cell wall and morphology of bacteria

    NASA Astrophysics Data System (ADS)

    Jiang, Hongyuan; Sun, Sean

    2010-03-01

    The peptidoglycan cell wall of bacteria is responsible for maintaining the cell shape and integrity. During the bacterial life cycle, the growth of the cell wall is affected by mechanical stress and osmotic pressure internal to the cell. We develop a theory to describe cell shape changes under the influence of mechanical forces. We find that the theory predicts a steady state size and shape for bacterial cells ranging from cocci to spirillum. Moreover, the theory suggest a mechanism by which bacterial cytoskeletal proteins such as MreB and crescentin can maintain the shape of the cell. The theory can also explain the several recent experiments on growing bacteria in micro-environments.

  10. Analysis of reach-scale elevation distribution in braided rivers: Definition of a new morphologic indicator and estimation of mean quantities

    NASA Astrophysics Data System (ADS)

    Redolfi, M.; Tubino, M.; Bertoldi, W.; Brasington, J.

    2016-08-01

    Understanding the role of external controls on the morphology of braided rivers is currently limited by the dearth of robust metrics to quantify and distinguish the diversity of channel form. Most existing measures are strongly dependent on river stage and unable to account for the three-dimensional complexity that is apparent in digital terrain models of braided rivers. In this paper, we introduce a simple, stage-independent morphological indicator that enables the analysis of reach-scale regime morphology as a function of slope, discharge, sediment size, and degree of confinement. The index is derived from the bed elevation frequency distribution and characterizes a statistical width-depth curve averaged longitudinally over multiple channel widths. In this way, we define a "synthetic channel" described by a simple parameter that embeds information about the river morphological complexity. Under the assumption of uniform flow, this approach can be extended to provide estimates of the reach-averaged shear stress distribution, bed load flux, and at-a-station-variability of wetted width. We test this approach using data from a wide range of labile channels including 58 flume experiments and three gravel bed braided rivers. Results demonstrate a strong relationship between the unit discharge and the shape of the elevation distribution, which varies between a U shape for typical single-thread confined channels and a Y shape for multithread reaches. Finally, we discuss the use of the metric as a diagnostic index of river condition that may be used to support inferences about the river morphological trajectory.

  11. Shape Transformation of the Nuclear Envelope during Closed Mitosis.

    PubMed

    Zhu, Qian; Zheng, Fan; Liu, Allen P; Qian, Jin; Fu, Chuanhai; Lin, Yuan

    2016-11-15

    The nuclear envelope (NE) in lower eukaryotes such as Schizosaccharomyces pombe undergoes large morphology changes during closed mitosis. However, which physical parameters are important in governing the shape evolution of the NE, and how defects in the dividing chromosomes/microtubules are reflected in those parameters, are fundamental questions that remain unresolved. In this study, we show that improper separation of chromosomes in genetically deficient cells leads to membrane tethering or asymmetric division in contrast to the formation of two equal-sized daughter nuclei in wild-type cells. We hypothesize that the poleward force is transmitted to the nuclear membrane through its physical contact with the separated sister chromatids at the two spindle poles. A theoretical model is developed to predict the morphology evolution of the NE where key factors such as the work done by the poleward force and bending and surface energies stored in the membrane have been taken into account. Interestingly, the predicted phase diagram, summarizing the dependence of nuclear shape on the size of the load transmission regions, and the pole-to-pole distance versus surface area relationship all quantitatively agree well with our experimental observations, suggesting that this model captures the essential physics involved in closed mitosis. Copyright © 2016 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  12. The phallus in Tettigoniidae (Insecta: Orthoptera: Ensifera): revision of morphology and terminology, and discussion on its taxonomic importance and evolution.

    PubMed

    Chamorro-Rengifo, Juliana; Lopes-Andrade, Cristiano

    2014-06-13

    The phallus in Tettigoniidae (katydids) is a structure informative relative to the systematics of the group. Despite this, it is often not considered in descriptions of taxa. The lack of adequate descriptions of phalli is not only a gap for sytematic and morphological studies, but postpones works on the evolution of copula. Here we study the exoskeletal morphology of the phallus in katydids, its components, and revised the terminology for them. We carried out dissections for morphological comparisons, and complement the observational information with published data. We stained phalli of katydids with chlorazol black, to better contrast membranous versus sclerotized components. We demonstrate that phallic components vary at specific, generic and suprageneric levels, and that internal and external components vary in number, shape, size and position. Currently there is little comparative data to support hypotheses on the evolution of this structure, but possibly the possession of a titillator is an ancestral condition. We identify additional sclerotized components, the sclerites of the ventral fold of the dorsal lobe, which can modify the shape and function of the titillator, being also important for understanding the evolution of the phallus. Potential functional relationships based on hypothetical morphological correlations between the shape of titillator and cerci are proposed, categorized in three main groups: (i) phallus devoid of titillator and cerci simple, (ii) titillator with bifurcated or paired sclerites, and cerci adapted for grasping, and (iii) titillator with single process and/or sclerite and cerci simple, sometimes with a pointed tip. Two explanations for these hypothetical morphological correlations and morphological variation are proposed: first, species with similar structures at the postabdomen would share similar copulatory behaviour, and second, more than one selective pressure would have acted over the structures of the postabdomen.

  13. Advance in quality assessment of Chinese materia medica using microscopic and morphological methods.

    PubMed

    Miao, Xiao-Su; Cui, Qing-Yu; Wang, Zhao-Yi; Liu, Xiao-Na; Zhao, An-Bang; Qiao, Yan-Jiang; Wu, Zhi-Sheng

    2017-09-01

    Quality evaluation plays a vital role in ensuring safety and effectiveness of Chinese materia medica (CMM). Microscopic and morphological technologies can be used to distinguish CMM's characteristics, such as shape, size, texture, section, and smell, for authenticity and quality control of CMM. The microscopic and morphological applications of novel micro-technology, colorimeter, and texture analyzer for CMM identification are summarized and the future prospect is discussed in this paper. Various styles and complex sources of CMM are systemically reviewed, including cormophyte medicinal materials, fruit and seeds, pollen grain, and spore materials. Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  14. Morphology-Induced Information Transfer in Bat Sonar

    NASA Astrophysics Data System (ADS)

    Reijniers, Jonas; Vanderelst, Dieter; Peremans, Herbert

    2010-10-01

    It has been argued that an important part of understanding bat echolocation comes down to understanding the morphology of the bat sound processing apparatus. In this Letter we present a method based on information theory that allows us to assess target localization performance of bat sonar, without a priori knowledge on the position, size, or shape of the reflecting target. We demonstrate this method using simulated directivity patterns of the frequency-modulated bat Micronycteris microtis. The results of this analysis indicate that the morphology of this bat’s sound processing apparatus has evolved to be a compromise between sensitivity and accuracy with the pinnae and the noseleaf playing different roles.

  15. In Vitro Morphological Characteristics of Pyrenophora tritici-repentis Isolates from Several Algerian Agro-Ecological Zones

    PubMed Central

    Benslimane, Hamida; Aouali, Souhila; Khalfi, Assia; Ali, Shaukat; Bouznad, Zouaoui

    2017-01-01

    Tan spot caused by the fungus Pyrenophora triticirepentis is a serious disease of wheat, which is on increase in recent years in Mediterranean region. In the field this fungus produces a diamond-shaped necrotic lesions with a yellow halo on wheat foliage. The objective of this study was to characterize and compare several monospore isolates of P. tritici-repentis collected from different infected wheat fields in various locations of Algeria, and find the morphological differences between them, if any. The results revealed wide morphologically variation among the isolates based on colony colors and texture, mycelial radial growth and conidial size. PMID:28381957

  16. Redescription of Corallobothrium solidum (Cestoda: Proteocephalidea) and erection of a new genus, Essexiella, for tapeworms from channel catfishes (Ictaluridae).

    PubMed

    Scholz, Tomáš; de Chambrier, Alain; Mariaux, Jean; Kuchta, Roman

    2011-12-01

    The proteocephalidean tapeworm, Corallobothrium solidum, type species of the genus, is redescribed on the basis of the examination of its type specimens and extensive material recently collected from Malapterurus electricus (type host). Some morphological characteristics of taxonomic importance are reported for the first time, such as the presence of semispherical (U-shaped) sphincters on the external (outer) margin of the suckers, a vaginal sphincter, a well-developed seminal receptacle, and a unique morphology of the eggs. Corallobothrium solidum differs from the 2 remaining species of the genus, both parasitic in channel catfishes (Ictaluridae), in its scolex shape, morphology of its suckers, presence of longitudinal and transverse grooves on the body surface, dense network of excretory canals in the apical part of the scolex, morphology of the eggs, and uterine development. The non-monophyletic nature of Corallobothrium is further supported by molecular data (partial sequences of the 28S rRNA gene) because C. solidum and the 2 remaining species from ictalurids do not form a monophyletic assemblage. Therefore, Essexiella n. gen. is proposed to accommodate Essexiella fimbriatum new comb. (type and only species; syn. Corallobothrium fimbriatum) from channel catfish. Essexiella n. gen. differs from Corallobothrium, Megathylacoides, and Megathylacus by the absence of a sphincter in the suckers, from Corallotaenia by the shape of the scolex and the number and shape of proglottids, and from Paraproteocephalus by the structure of the uterus. The diagnosis of Corallobothrium, which becomes monotypic and restricted to electric catfishes in Africa, is emended. The remaining species of Corallobothrium, Corallobothrium parafimbriatum, is tentatively transferred to Corallotaenia as Corallotaenia parafimbriata n. comb., based on molecular data, small size of the strobila, and shape of the scolex.

  17. A Genome-Wide Association Study Identifies Five Loci Influencing Facial Morphology in Europeans

    PubMed Central

    Liu, Fan; van der Lijn, Fedde; Schurmann, Claudia; Zhu, Gu; Chakravarty, M. Mallar; Hysi, Pirro G.; Wollstein, Andreas; Lao, Oscar; de Bruijne, Marleen; Ikram, M. Arfan; van der Lugt, Aad; Rivadeneira, Fernando; Uitterlinden, André G.; Hofman, Albert; Niessen, Wiro J.; Homuth, Georg; de Zubicaray, Greig; McMahon, Katie L.; Thompson, Paul M.; Daboul, Amro; Puls, Ralf; Hegenscheid, Katrin; Bevan, Liisa; Pausova, Zdenka; Medland, Sarah E.; Montgomery, Grant W.; Wright, Margaret J.; Wicking, Carol; Boehringer, Stefan; Spector, Timothy D.; Paus, Tomáš; Martin, Nicholas G.; Biffar, Reiner; Kayser, Manfred

    2012-01-01

    Inter-individual variation in facial shape is one of the most noticeable phenotypes in humans, and it is clearly under genetic regulation; however, almost nothing is known about the genetic basis of normal human facial morphology. We therefore conducted a genome-wide association study for facial shape phenotypes in multiple discovery and replication cohorts, considering almost ten thousand individuals of European descent from several countries. Phenotyping of facial shape features was based on landmark data obtained from three-dimensional head magnetic resonance images (MRIs) and two-dimensional portrait images. We identified five independent genetic loci associated with different facial phenotypes, suggesting the involvement of five candidate genes—PRDM16, PAX3, TP63, C5orf50, and COL17A1—in the determination of the human face. Three of them have been implicated previously in vertebrate craniofacial development and disease, and the remaining two genes potentially represent novel players in the molecular networks governing facial development. Our finding at PAX3 influencing the position of the nasion replicates a recent GWAS of facial features. In addition to the reported GWA findings, we established links between common DNA variants previously associated with NSCL/P at 2p21, 8q24, 13q31, and 17q22 and normal facial-shape variations based on a candidate gene approach. Overall our study implies that DNA variants in genes essential for craniofacial development contribute with relatively small effect size to the spectrum of normal variation in human facial morphology. This observation has important consequences for future studies aiming to identify more genes involved in the human facial morphology, as well as for potential applications of DNA prediction of facial shape such as in future forensic applications. PMID:23028347

  18. Forced-folding by laccolith and saucer-shaped sill intrusions on the Earth, planets and icy satellites

    NASA Astrophysics Data System (ADS)

    Michaut, Chloé

    2017-04-01

    Horizontal intrusions probably initially start as cracks, with negligible surface deformation. Once their horizontal extents become large enough compared to their depths, they make room for themselves by lifting up their overlying roofs, creating characteristic surface deformations that can be observed at the surface of planets. We present a model where magma flows below a thin elastic overlying layer characterized by a flexural wavelength Λ and study the dynamics and morphology of such a magmatic intrusion. Our results show that, depending on its size, the intrusion present different shapes and thickness-to-radius relationships. During a first phase, elastic bending of the overlying layer is the main source of driving pressure in the flow; the pressure decreases as the flow radius increases, the intrusion is bell-shaped and its thickness is close to being proportional to its radius. When the intrusion radius becomes larger than 4 times Λ, the flow enters a gravity current regime and progressively develops a pancake shape with a flat top. We study the effect of topography on flow spreading in particular in the case where the flow is constrained by a lithostatic barrier within a depression, such as an impact crater on planets or a caldera on Earth. We show that the resulting shape for the flow depends on the ratio between the flexural wavelength of the layer overlying the intrusion and the depression radius. The model is tested against terrestrial data and is shown to well explain the size and morphology of laccoliths and saucer-shaped sills on Earth. We use our results to detect and characterize shallow solidified magma reservoirs in the crust of terrestrial planets and potential shallow water reservoirs in the ice shell of icy satellites.

  19. Analysis of current-driven oscillatory dynamics of single-layer homoepitaxial islands on crystalline conducting substrates

    NASA Astrophysics Data System (ADS)

    Dasgupta, Dwaipayan; Kumar, Ashish; Maroudas, Dimitrios

    2018-03-01

    We report results of a systematic study on the complex oscillatory current-driven dynamics of single-layer homoepitaxial islands on crystalline substrate surfaces and the dependence of this driven dynamical behavior on important physical parameters, including island size, substrate surface orientation, and direction of externally applied electric field. The analysis is based on a nonlinear model of driven island edge morphological evolution that accounts for curvature-driven edge diffusion, edge electromigration, and edge diffusional anisotropy. Using a linear theory of island edge morphological stability, we calculate a critical island size at which the island's equilibrium edge shape becomes unstable, which sets a lower bound for the onset of time-periodic oscillatory dynamical response. Using direct dynamical simulations, we study the edge morphological dynamics of current-driven single-layer islands at larger-than-critical size, and determine the actual island size at which the migrating islands undergo a transition from steady to time-periodic asymptotic states through a subcritical Hopf bifurcation. At the highest symmetry of diffusional anisotropy examined, on {111} surfaces of face-centered cubic crystalline substrates, we find that more complex stable oscillatory states can be reached through period-doubling bifurcation at island sizes larger than those at the Hopf points. We characterize in detail the island morphology and dynamical response at the stable time-periodic asymptotic states, determine the range of stability of these oscillatory states terminated by island breakup, and explain the morphological features of the stable oscillating islands on the basis of linear stability theory.

  20. Effects of experimental warming on survival, phenology and morphology of an aquatic insect (Odonata)

    PubMed Central

    McCauley, Shannon J.; Hammond, John I.; Frances, Dachin N.; Mabry, Karen E.

    2014-01-01

    1. Organisms can respond to changing climatic conditions in multiple ways including changes in phenology, body size or morphology, and range shifts. Understanding how developmental temperatures affect insect life-history timing and morphology is crucial because body size and morphology affect multiple aspects of life history, including dispersal ability, while phenology can shape population performance and community interactions. 2. We experimentally assessed how developmental temperatures experienced by aquatic larvae affected survival, phenology, and adult morphology of dragonflies (Pachydiplax longipennis). Larvae were reared under 3 environmental temperatures: ambient, +2.5 °C, and +5 °C, corresponding to temperature projections for our study area 50 and 100 years in the future, respectively. Experimental temperature treatments tracked naturally-occurring variation. 3. We found clear effects of temperature in the rearing environment on survival and phenology: dragonflies reared at the highest temperatures had the lowest survival rates, and emerged from the larval stage approximately 3 weeks earlier than animals reared at ambient temperatures. There was no effect of rearing temperature on overall body size. Although neither the relative wing nor thorax size was affected by warming, a non-significant trend towards an interaction between sex and warming in relative thorax size suggests that males may be more sensitive to warming than females, a pattern that should be investigated further. 4. Warming strongly affected survival in the larval stage and the phenology of adult emergence. Understanding how warming in the developmental environment affects later life-history stages is critical to interpreting the consequences of warming for organismal performance. PMID:26028806

  1. Comparison of morphology and phase composition of hydroxyapatite nanoparticles sonochemically synthesized with dual- or single-frequency ultrasonic reactor

    NASA Astrophysics Data System (ADS)

    Deng, Shi-ting; Yu, Hong; Liu, Di; Bi, Yong-guang

    2017-10-01

    To investigate how a dual- or single-frequency ultrasonic reactor changes the morphology and phase composition of hydroxyapatite nanoparticles (nHAPs), we designed and constructed the preparation of nHAPs using dual- or single-frequency ultrasonic devices, i.e., the single frequency ultrasonic generator with ultrasonic horn (25 kHz), the ultrasonic bath (40 kHz) and the dual-frequency sonochemical systems combined with the ultrasonic horn and the ultrasonic bath simultaneously (25 + 40 kHz). The results showed that the sonicated samples displayed a more uniform shape with less agglomeration than non-sonicated sample. The rod-shaped particles with 1.66 stoichiometry and without a second phase were synthesized successfully in the ultrasonic bath or horn systems. The nHAPs obtained from the dual-frequency ultrasonic systems exhibited a regular rod-shaped structure with better dispersion and more uniform shapes than those of obtained in either ultrasonic bath or horn systems. Additionally, the size of rod-shaped particles obtained in the dual-frequency ultrasound with a mean width of 35 nm and a mean length of 64 nm was smaller than other samples. A possible mechanism is that the dual-frequency ultrasound significantly enhances the cavitation yield over single frequency ultrasound and thus improves the dispersion of particles and reduces the size of the crystals. In addition, irregular holes can be observed in the nanoparticles obtained in the dual-frequency ultrasound. Therefore, the dual-frequency ultrasonic systems are expected to become a convenient, efficient and environmentally friendly synthetic technology to obtain well-defined nHAPs for specific biomedical applications.

  2. 3D surface reconstruction and visualization of the Drosophila wing imaginal disc at cellular resolution

    NASA Astrophysics Data System (ADS)

    Bai, Linge; Widmann, Thomas; Jülicher, Frank; Dahmann, Christian; Breen, David

    2013-01-01

    Quantifying and visualizing the shape of developing biological tissues provide information about the morphogenetic processes in multicellular organisms. The size and shape of biological tissues depend on the number, size, shape, and arrangement of the constituting cells. To better understand the mechanisms that guide tissues into their final shape, it is important to investigate the cellular arrangement within tissues. Here we present a data processing pipeline to generate 3D volumetric surface models of epithelial tissues, as well as geometric descriptions of the tissues' apical cell cross-sections. The data processing pipeline includes image acquisition, editing, processing and analysis, 2D cell mesh generation, 3D contourbased surface reconstruction, cell mesh projection, followed by geometric calculations and color-based visualization of morphological parameters. In their first utilization we have applied these procedures to construct a 3D volumetric surface model at cellular resolution of the wing imaginal disc of Drosophila melanogaster. The ultimate goal of the reported effort is to produce tools for the creation of detailed 3D geometric models of the individual cells in epithelial tissues. To date, 3D volumetric surface models of the whole wing imaginal disc have been created, and the apicolateral cell boundaries have been identified, allowing for the calculation and visualization of cell parameters, e.g. apical cross-sectional area of cells. The calculation and visualization of morphological parameters show position-dependent patterns of cell shape in the wing imaginal disc. Our procedures should offer a general data processing pipeline for the construction of 3D volumetric surface models of a wide variety of epithelial tissues.

  3. Light-Induced Indeterminacy Alters Shade-Avoiding Tomato Leaf Morphology1[OPEN

    PubMed Central

    Chitwood, Daniel H.; Kumar, Ravi; Ranjan, Aashish; Pelletier, Julie M.; Townsley, Brad T.; Ichihashi, Yasunori; Martinez, Ciera C.; Zumstein, Kristina; Harada, John J.; Maloof, Julin N.; Sinha, Neelima R.

    2015-01-01

    Plants sense the foliar shade of competitors and alter their developmental programs through the shade-avoidance response. Internode and petiole elongation, and changes in overall leaf area and leaf mass per area, are the stereotypical architectural responses to foliar shade in the shoot. However, changes in leaf shape and complexity in response to shade remain incompletely, and qualitatively, described. Using a meta-analysis of more than 18,000 previously published leaflet outlines, we demonstrate that shade avoidance alters leaf shape in domesticated tomato (Solanum lycopersicum) and wild relatives. The effects of shade avoidance on leaf shape are subtle with respect to individual traits but are combinatorially strong. We then seek to describe the developmental origins of shade-induced changes in leaf shape by swapping plants between light treatments. Leaf size is light responsive late into development, but patterning events, such as stomatal index, are irrevocably specified earlier. Observing that shade induces increases in shoot apical meristem size, we then describe gene expression changes in early leaf primordia and the meristem using laser microdissection. We find that in leaf primordia, shade avoidance is not mediated through canonical pathways described in mature organs but rather through the expression of KNOTTED1-LIKE HOMEOBOX and other indeterminacy genes, altering known developmental pathways responsible for patterning leaf shape. We also demonstrate that shade-induced changes in leaf primordium gene expression largely do not overlap with those found in successively initiated leaf primordia, providing evidence against classic hypotheses that shaded leaf morphology results from the prolonged production of juvenile leaf types. PMID:26381315

  4. Morphometric assessment of pterosaur jaw disparity

    NASA Astrophysics Data System (ADS)

    Navarro, Charlie A.; Martin-Silverstone, Elizabeth; Stubbs, Thomas L.

    2018-04-01

    Pterosaurs were a successful group of Mesozoic flying reptiles. They were the first vertebrate group to achieve powered flight and varied enormously in morphology and ecology, occupying a variety of niches and developing specialized feeding strategies. Ecomorphological principles suggest this variation should be reflected by great morphological diversity in the lower jaw, given that the mandible served as the primary apparatus for prey acquisition. Here we present the first study of mandibular shape disparity in pterosaurs and aim to characterize major aspects of variation. We use a combination of geometric morphometric approaches, incorporating both outline analysis using elliptical Fourier analysis and semi-landmark approaches. Our results show that morphological convergence is prevalent and many pterosaurs, belonging to diverse dietary groups and subclades, overlap in morphospace and possessed relatively simple `rod-shaped' jaws. There is no clear trend of size distributions in pterosaur mandibular morphospace, and larger forms are widely distributed. Additionally, there is limited functional signal within pterosaur lower jaw morphospace. Instead, the development of a large anterior ventral crest represents the major component of disparity. This suggests that a socio-sexual trait was a key driver for innovation in pterosaur lower jaw shape.

  5. Morphometric assessment of pterosaur jaw disparity.

    PubMed

    Navarro, Charlie A; Martin-Silverstone, Elizabeth; Stubbs, Thomas L

    2018-04-01

    Pterosaurs were a successful group of Mesozoic flying reptiles. They were the first vertebrate group to achieve powered flight and varied enormously in morphology and ecology, occupying a variety of niches and developing specialized feeding strategies. Ecomorphological principles suggest this variation should be reflected by great morphological diversity in the lower jaw, given that the mandible served as the primary apparatus for prey acquisition. Here we present the first study of mandibular shape disparity in pterosaurs and aim to characterize major aspects of variation. We use a combination of geometric morphometric approaches, incorporating both outline analysis using elliptical Fourier analysis and semi-landmark approaches. Our results show that morphological convergence is prevalent and many pterosaurs, belonging to diverse dietary groups and subclades, overlap in morphospace and possessed relatively simple 'rod-shaped' jaws. There is no clear trend of size distributions in pterosaur mandibular morphospace, and larger forms are widely distributed. Additionally, there is limited functional signal within pterosaur lower jaw morphospace. Instead, the development of a large anterior ventral crest represents the major component of disparity. This suggests that a socio-sexual trait was a key driver for innovation in pterosaur lower jaw shape.

  6. Electron tomography and 3D molecular simulations of platinum nanocrystals

    NASA Astrophysics Data System (ADS)

    Florea, Ileana; Demortière, Arnaud; Petit, Christophe; Bulou, Hervé; Hirlimann, Charles; Ersen, Ovidiu

    2012-07-01

    This work reports on the morphology of individual platinum nanocrystals with sizes of about 5 nm. By using the electron tomography technique that gives 3D spatial selectivity, access to quantitative information in the real space was obtained. The morphology of individual nanoparticles was characterized using HAADF-STEM tomography and it was shown to be close to a truncated octahedron. Using molecular dynamics simulations, this geometrical shape was found to be the one minimizing the nanocrystal energy. Starting from the tomographic reconstruction, 3D crystallographic representations of the studied Pt nanocrystals were obtained at the nanometer scale, allowing the quantification of the relative amount of the crystallographic facets present on the particle surface.This work reports on the morphology of individual platinum nanocrystals with sizes of about 5 nm. By using the electron tomography technique that gives 3D spatial selectivity, access to quantitative information in the real space was obtained. The morphology of individual nanoparticles was characterized using HAADF-STEM tomography and it was shown to be close to a truncated octahedron. Using molecular dynamics simulations, this geometrical shape was found to be the one minimizing the nanocrystal energy. Starting from the tomographic reconstruction, 3D crystallographic representations of the studied Pt nanocrystals were obtained at the nanometer scale, allowing the quantification of the relative amount of the crystallographic facets present on the particle surface. Electronic supplementary information (ESI) available. See DOI: 10.1039/c2nr30990d

  7. Exploring phylogenetic and functional signals in complex morphologies: the hamate of extant anthropoids as a test-case study.

    PubMed

    Almécija, Sergio; Orr, Caley M; Tocheri, Matthew W; Patel, Biren A; Jungers, William L

    2015-01-01

    Three-dimensional geometric morphometrics (3DGM) is a powerful tool for capturing and visualizing the "pure" shape of complex structures. However, these shape differences are sometimes difficult to interpret from a functional viewpoint, unless specific approaches (mostly based on biomechanical modeling) are employed. Here, we use 3DGM to explore the complex shape variation of the hamate, the disto-ulnar wrist bone, in anthropoid primates. Major trends of shape variation are explored using principal components analysis along with analyses of shape and size covariation. We also evaluate the phylogenetic patterning of hamate shape by plotting an anthropoid phylogenetic tree onto the shape space (i.e., phylomorphospace) and test against complete absence of phylogenetic signal using posterior permutation. Finally, the covariation of hamate shape and locomotor categories is explored by means of 2-block partial least squares (PLS) using shape coordinates and a matrix of data on arboreal locomotor behavior. Our results show that 3DGM is a valuable and versatile tool for characterizing the shape of complex structures such as wrist bones in anthropoids. For the hamate, a significant phylogenetic pattern is found in both hamate shape and size, indicating that closely related taxa are typically the most similar in hamate form. Our allometric analyses show that major differences in hamate shape among taxa are not a direct consequence of differences in hamate size. Finally, our PLS indicates a significant covariation of hamate shape and different types of arboreal locomotion, highlighting the relevance of this approach in future 3DGM studies seeking to capture a functional signal from complex biological structures. © 2014 Wiley Periodicals, Inc.

  8. A morphological and functional basis for maximum prey size in piscivorous fishes

    PubMed Central

    Bellwood, David R.

    2017-01-01

    Fish predation is important in shaping populations and community structure in aquatic systems. These predator-prey interactions can be influenced by environmental, behavioural and morphological factors. Morphological constraints influence the feeding performance of species, and interspecific differences can thus affect patterns of resource use. For piscivorous fishes that swallow prey whole, feeding performance has traditionally been linked to three key morphological constraints: oral gape, pharyngeal gape, and the cleithral gape. However, other constraints may be important. We therefore examine 18 potential morphological constraints related to prey capture and processing, on four predatory species (Cephalopholis urodeta, Paracirrhites forsteri, Pterois volitans, Lates calcarifer). Aquarium-based experiments were then carried out to determine capture and processing behaviour and maximum prey size in two focal species, C. urodeta and P. forsteri. All four species showed a progressive decrease in gape measurements from anterior to posterior with oral gape ≥ buccal ≥ pharyngeal ≥ pectoral girdle ≥ esophagus ≥ stomach. C. urodeta was able to process prey with a maximum depth of 27% of the predators’ standard length; for P. forsteri it was 20%. C. urodeta captured prey head-first in 79% of successful strikes. In P. forsteri head-first was 16.6%, mid-body 44.4%, and tail-first 38.8%. Regardless of capture mode, prey were almost always swallowed head first and horizontally in both focal species. Most internal measurements appeared too small for prey to pass through. This may reflect the compressibility of prey, i.e. their ability to be dorsoventrally compressed during swallowing movements. Despite examining all known potential morphological constraints on prey size, horizontal maxillary oral gape in a mechanically stretched position appears to be the main morphological variable that is likely to affect maximum prey size and resource use by these predatory species. PMID:28886161

  9. Fish-borne Zoonotic Trematode Metacercariae in the Republic of Korea

    PubMed Central

    2009-01-01

    The prevalence of fish-borne trematodes (FBT), including Clonorchis sinensis, is still high in riverside areas of the Republic of Korea. The author reviewed the detection and identification methods, differential keys, fish intermediate hosts, and morphological characteristics of FBT metacercariae. FBT metacercariae found in freshwater fish are classified mainly into 4 families, i.e., Opisthorchiidae, Heterophyidae, Echinostomatidae, and Clinostomidae. The metacercariae of C. sinensis, found in 40 species of freshwater fish, are elliptical and 0.15-0.17 × 0.13-0.15 mm in size, have nearly equal sized oral and ventral suckers, brownish pigment granules, and an O-shaped excretory bladder. Their general morphologies are similar to those of Metorchis orientalis (except in the thickness of the cyst wall). Metagonimus spp. (M. yokogawai, M. takahashii, and M. miyatai) metacercariae are subglobular or disc-shaped, and 0.14-0.16 mm in diameter. They have yellow-brownish pigment granules, a ventral sucker deflectively located from median, and a V-shaped excretory bladder. The metacercariae and fish intermediate hosts of Centrocestus armatus, Clinostomum complanatum, and 3 echinostomatid flukes (Echinostoma hortense, E. cinetorchis, and Echinochasmus japonicus) were summarized. FBT metacercariae detected in brackish water fish are mainly members of the Heterophyidae. The morphological characters, identification keys, and fish intermediate hosts of 7 species (Heterophyes nocens, Heterophyopsis continua, Pygidiopsis summa, Stellantchasmus falcatus, Stictodora fuscata, Stictodora lari, and Acanthotrema felis) were also reviewed. The contents treated in this study will provide assistance at the laboratory bench level to those working on recovery of metacercariae from fish hosts and identifying them. PMID:19885326

  10. Plasticity as Phenotype: G x E Interaction in a Freshwater Snail

    NASA Astrophysics Data System (ADS)

    Brunkow, P. E.; Calloway, S. A.

    2005-05-01

    Plasticity in morphological development allows species to accommodate environmental variation experienced during growth; however, genetic variation for phenotypic plasticity per se has been relatively under-studied. We utilized the well-documented plastic response of shell development to predator cues in a freshwater snail to quantify genetic variation for plasticity in growth rate and shell shape. Field-caught pairs of snails reproduced in the laboratory to create families of full siblings, which were then divided and allowed to grow in control and predator cue treatments. Predator (crayfish) cues had significant effects on both size-corrected growth rate and shell shape; family identity also significantly affected both final shell shape and growth rate. The interaction between predator treatment and family identity significantly affected snail growth rate but not final shell shape, suggesting genetic variation in the plastic response to predator cues for a physiological variable (growth rate) but not for a variable known to mechanically reduce the risk of predation (shell shape), at least in this population of snails. The possibility that risk of multiple modes of predation (i.e., both fish and crayfish) in some populations might maintain genetic variation in morphological plasticity is discussed.

  11. Evolution of Skull and Mandible Shape in Cats (Carnivora: Felidae)

    PubMed Central

    Christiansen, Per

    2008-01-01

    The felid family consists of two major subgroups, the sabretoothed and the feline cats, to which all extant species belong, and are the most anatomically derived of all carnivores for predation on large prey with a precision killing bite. There has been much controversy and uncertainty about why the skulls and mandibles of sabretoothed and feline cats evolved to become so anatomically divergent, but previous models have focused on single characters and no unifying hypothesis of evolutionary shape changes has been formulated. Here I show that the shape of the skull and mandible in derived sabrecats occupy entirely different positions within overall morphospace from feline cats, and that the evolution of skull and mandible shape has followed very different paths in the two subgroups. When normalised for body-size differences, evolution of bite forces differ markedly in the two groups, and are much lower in derived sabrecats, and they show a significant relationship with size and cranial shape, whereas no such relationship is present in feline cats. Evolution of skull and mandible shape in modern cats has been governed by the need for uniform powerful biting irrespective of body size, whereas in sabrecats, shape evolution was governed by selective pressures for efficient predation with hypertrophied upper canines at high gape angles, and bite forces were secondary and became progressively weaker during sabrecat evolution. The current study emphasises combinations of new techniques for morphological shape analysis and biomechanical studies to formulate evolutionary hypotheses for difficult groups. PMID:18665225

  12. Skull shape and size variation within and between mendocinus and torquatus groups in the genus Ctenomys (Rodentia: Ctenomyidae) in chromosomal polymorphism context

    PubMed Central

    Fornel, Rodrigo; Cordeiro-Estrela, Pedro; de Freitas, Thales Renato O.

    2018-01-01

    Abstract We tested the association between chromosomal polymorphism and skull shape and size variation in two groups of the subterranean rodent Ctenomys. The hypothesis is based on the premise that chromosomal rearrangements in small populations, as it occurs in Ctenomys, produce reproductive isolation and allow the independent diversification of populations. The mendocinus group has species with low chromosomal diploid number variation (2n=46-48), while species from the torquatus group have a higher karyotype variation (2n=42-70). We analyzed the shape and size variation of skull and mandible by a geometric morphometric approach, with univariate and multivariate statistical analysis in 12 species from mendocinus and torquatus groups of the genus Ctenomys. We used 763 adult skulls in dorsal, ventral, and lateral views, and 515 mandibles in lateral view and 93 landmarks in four views. Although we expected more phenotypic variation in the torquatus than the mendocinus group, our results rejected the hypothesis of an association between chromosomal polymorphism and skull shape and size variation. Moreover, the torquatus group did not show more variation than mendocinus. Habitat heterogeneity associated to biomechanical constraints and other factors like geography, phylogeny, and demography, may affect skull morphological evolution in Ctenomys. PMID:29668015

  13. Facile one step synthesis of novel TiO2 nanocoral by sol-gel method using Aloe vera plant extract

    NASA Astrophysics Data System (ADS)

    Venkatesh, K. S.; Krishnamoorthi, S. R.; Palani, N. S.; Thirumal, V.; Jose, Sujin P.; Wang, Fu-Ming; Ilangovan, R.

    2015-05-01

    Titanium oxide (TiO2) nanoparticles (NPs) were synthesized by sol gel method using Aloe vera plant extract as a biological capping agent and a cauliflower-nanocoral morphology was observed in this technique. The assynthesized TiO2 nanopowder was calcined at a range of temperatures (300-600 °C) for 1 h. The influence of A. vera plant extract on the thermal, structural and morphological properties of TiO2 nanopowder was evaluated. Thermogravimetric analysis/differential thermal analysis was employed to study the thermal properties of the assynthesized TiO2 nanopowder. The crystallinity, phase transformation and the crystallite size of the calcined samples were studied by X-ray diffraction technique. XRD result confirmed the presence of TiO2 with anatase phase. FT Raman spectra showed the Raman active modes pertaining to the TiO2 anatase phase and Raman band shift was also observed with respect to particle size variation. The different functional group vibrations of as dried pure A. vera plant extract were compared with the mixture of TiO2 and A. vera plant extract by FT-IR analysis. The scanning electron microscopy images apparently showed the formation of spherical shaped NPs and also it demonstrated the effect of A. vera plant extract on the reduction of particles size. The surface area of the TiO2 NPs was measured through Brunauer-Emmett-Teller analysis. Transmission electron microscopy images ascertained that the spherical shaped TiO2 NPs were formed with cauliflower-nanocoral morphology decorated with nanopolyps with the size range between 15 and 30 nm.

  14. Barlenses and X-shaped features compared: two manifestations of boxy/peanut bulges

    NASA Astrophysics Data System (ADS)

    Laurikainen, E.; Salo, H.

    2017-02-01

    Aims: We study the morphological characteristics of boxy/peanut-shaped bulges. In particular, we are interested to determine whether most of the flux associated with bulges in galaxies with masses similar to those of the Milky Way at redshift z 0 might belong to the vertically thick inner part of the bar, in a similar manner as in the Milky Way itself. At high galaxy inclinations, these structures are observed as boxy/peanut/X-shaped features, and when the view is near to face-on, they are observed as barlenses. We also study the possibility that bulges in some fraction of unbarred galaxies might form in a similar manner as the bulges in barred galaxies. Methods: We used the Spitzer Survey of Stellar Structure in Galaxies (S4G) and the Near-IR S0 galaxy Survey (NIRS0S) to compile complete samples of galaxies with barlenses (N = 85) and X-shaped features (N = 88). A sample of unbarred galaxies (N = 41) is also selected. For all 214 galaxies unsharp mask images were created, used to recognize the X-shaped features and to measure their linear sizes. To detect possible boxy isophotes (using the B4-parameter), we also performed an isophotal analysis for the barlens galaxies. We use recently published N-body simulations: the models that exhibit boxy/peanut/X/barlens morphologies are viewed from isotropically chosen directions that cover the full range of galaxy inclinations in the sky. The synthetic images were analyzed in a similar manner as the observations. Results: This is the first time that the observed properties of barlenses and X-shaped features are directly compared across a wide range of galaxy inclinations. A comparison with the simulation models shows that the differences in their apparent sizes, a/rbar ≳ 0.5 for barlenses and a/rbar ≲ 0.5 for X-shapes, can be explained by projection effects. Observations at various inclinations are consistent with intrinsic abl ≈ aX ≈ 0.5rbar: here intrinsic size means the face-on semimajor axis length for bars and barlenses, and the semilength of the X-shape when the bar is viewed exactly edge-on. While X-shapes are quite common at intermediate galaxy inclinations (for I = 40°-60° their frequency is about half that of barlenses), they are seldom observed at smaller inclinations. This is consistent with our simulation models, which have a small compact classical bulge that produces a steep inner rotation slope, whereas bulgeless shallow rotation curve models predict that X-shapes should be visible even in a face-on geometry. The steep rotation curve models are also consistent with the observed trend that B4 is positive at low inclination and with negative values for I ≳ 40°-60°; this implies boxy isophotes. In total, only about one quarter of the barlenses (with I ≤ 60°) show boxy isophotes. Conclusions: Our analyses are consistent with the idea that barlenses and X-shaped features are physically the same phenomenon. However, the observed nearly round face-on barlens morphology is expected only when at least a few percent of the disk mass is located in a central component, within a region much smaller than the size of the barlens itself. Barlenses contribute to secular evolution of galaxies, and might even act as a transition phase between barred and unbarred galaxies. We also discuss that the wide range of stellar population ages obtained for the photometric bulges in the literature are consistent with our interpretation.

  15. Forensic implications of the variation in morphology of marginal serrations on the teeth of the great white shark.

    PubMed

    Nambiar, P; Brown, K A; Bridges, T E

    1996-06-01

    The teeth of the Great White Shark have been examined to ascertain whether there is any commonality in the arrangement or number of the marginal serrations (peaks) or, indeed, whether individual sharks have a unique pattern of shapes or size of the peaks. The teeth of the White Shark are characteristic in size and shape with serrations along almost the entire mesial and distal margins. This study has revealed no consistent pattern of size or arrangement of the marginal serrations that was sufficiently characteristic within an individual shark to serve as a reliable index of identification of a tooth as originating from that particular shark. Nonetheless, the serrations are sufficiently distinctive to enable the potential identification of an individual tooth as having been the cause of a particular bitemark.

  16. Evolution of morphological and optical properties of self-assembled Ag nanostructures on c-plane sapphire (0001) by the precise control of deposition amount

    NASA Astrophysics Data System (ADS)

    Kunwar, Sundar; Li, Ming-Yu; Pandey, Puran; Sui, Mao; Zhang, Quanzhen; Lee, Jihoon

    2016-12-01

    Silver (Ag) nanoparticles (NPs) have been widely adapted in various optoelectronic and sensing applications due to the size, shape and density dependent tunable properties. In this work, the systematic control of the size, configuration and density of self-assembled Ag nanostructures on c-plane sapphire (0001) is demonstrated through the solid state dewetting process by the variation of deposition amount (DA) at two distinctive temperature of 400 °C and 650 °C. The corresponding morphological evolution of Ag nanostructures is systematically discussed based on the diffusion, Volmer-Weber and coalescence growth model. In specific, at the relatively lower temperature of 400 °C, the Ag nanostructures evolve in three distinctive regimes based on the DA control: i.e. the dome-shaped Ag NPs between 2 and 14 nm (regime I), the irregular nano-mounds (NMs) between 20 and 40 nm (regime II), and the coalescence of Ag NMs into a layer between 60 and 200 nm (regime III). Meanwhile, at the relatively higher temperature of 650 °C, due to growth regime shift induced by the enhanced surface diffusion based on the increased thermal energy, the connected Ag NMs are resulted even at higher DAs and evolve along with the gradually increased DAs. The evolution of optical properties such as average reflectivity, plasmonic absorption band and the reflectance maxima (peaks) very sensitively respond to the evolution of size, shape and spacing of Ag nanostructures and discussed based on the surface plasmon, reflection and scattering. Specifically, the dome-shaped configuration exhibits strong absorption in the NIR region and weak absorption in visible region while the elongated NMs show the enhanced absorption in visible region. Furthermore, the Raman spectra (A 1g vibrational mode) of the Ag nanostructures demonstrate the strong correlation with the evolution of size, density and surface coverage of the nanostructures.

  17. Dry powder inhaler performance of spray dried mannitol with tailored surface morphologies as carrier and salbutamol sulphate.

    PubMed

    Mönckedieck, M; Kamplade, J; Fakner, P; Urbanetz, N A; Walzel, P; Steckel, H; Scherließ, R

    2017-05-30

    Nowadays, dry powder inhalation as applied in the therapy of pulmonary diseases is known as a very effective route of drug delivery to the lungs. Here, the system of coarse carrier and fine drug particles attached to the carrier surface has successfully been applied to overcome the cohesiveness of small drug particles. Particle properties of both carrier and drug are known to affect drug dispersion as has widely been discussed for lactose monohydrate and various drugs. This study utilises particle-engineered mannitol as an alternative carrier to discover the effect of mannitol carrier particle properties like particle shape, surface roughness, flowability or particle size on aerodynamic performance during inhalation. Spray drying as a technique to accurately control those properties was chosen for the generation of carrier sizes between 50 and 80 μm and different morphologies and therefore various carrier flowabilities. A set of these carriers has then been blended with different spray dried and jet-milled qualities of salbutamol sulphate as model drug to examine the influence of carrier particle properties on aerodynamic behaviour and at the same time to cover the effect of drug particle properties on particle-particle interactions. This experimental setup allowed a general view on how drug and carrier properties affect the Fine Particle Fraction (FPF) as indicator for inhalation performance and gave the first study to distinguish between mannitol carrier particle shape and surface roughness. Further it was possible to relate carrier particle size and shape to drug accumulation and detachment mechanisms during inhalation as size and shape had the main influence on drug detachment. The addition of jet-milled mannitol fines provided an initial insight into the improving effect of ternary powder blends as has been intensively studied for lactose monohydrate but not for mannitol yet. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Ontogeny of the female femur: geometric morphometric analysis applied on current living individuals of a Spanish population

    PubMed Central

    Pujol, Aniol; Rissech, Carme; Ventura, Jacint; Badosa, Joaquim; Turbón, Daniel

    2014-01-01

    In this study we describe the development of the female femur based on the analysis of high-resolution radiographic images by means of geometric morphometrics, while assessing the usefulness of this method in these kinds of studies. The material analysed consisted of digital images in DICOM format (telemetries), corresponding to 184 left femora in anterior view, obtained from the database of the Hospital Sant Joan de Déu of Barcelona (Spain). Bones analysed corresponded to individuals from 9 to 14 years old. Size and shape variation of the entire femur was quantified by 22 two-dimensional landmarks. Landmark digitisation errors were assessed using Procrustes anova test. Centroid size (CS) variation with age was evaluated by an anova test. Shape variation was assessed by principal component analysis. A mancova test between the first five principal components and age, using the CS as covariable, was applied. Results indicated that both size and shape vary significantly with age. Several age-related shape changes remained significant after removing the allometric effect. In general, an increase in the robustness of the bone and noticeable phenotypic changes in certain areas of the femur were observed. During growth in the proximal region of the femur, the collo-diaphyseal angle decreases, the neck of the femur widens and the fovea moves to a lower position, standing more in line with the plane of the neck. Likewise, the size of the greater and lesser trochanters increase. In the distal region, a significant increase of epiphyseal dimensions was recorded, mainly in the medial condyle. The angular remodelling of the neck and the bicondylar region of the femur in females continues until 13 years old. The information provided in the present study increases our knowledge on the timing and morphology of the femur during development, and in particular the morphology of the different femoral ossification centres during development. PMID:24975495

  19. New self-limiting assembly model for Si quantum rings on Si(100).

    PubMed

    Yu, L W; Chen, K J; Song, J; Xu, J; Li, W; Li, X F; Wang, J M; Huang, X F

    2007-04-20

    We propose a new self-limiting assembly model for Si quantum rings on Si(100) where the ring's formation and evolution are driven by a growth-etching competition mechanism. The as-grown ring structure in a plasma enhanced chemical vapor deposition system has excellent rotational symmetry and superior morphology with a typical diameter, edge width, and height of 150-300, 10, and 5 nm, respectively. Based on this model, the size and morphology can be controlled well by simply tuning the timing procedure. We suggest that this growth model is not limited to certain material system, but provides a general scheme to control and tailor the self-assembly nanostructures into the desired size, shape, and complexity.

  20. Impact of physical and chemical parameters on the hydroxyapatite nanopowder synthesized by chemical precipitation method

    NASA Astrophysics Data System (ADS)

    Thu Trang Pham, Thi; Phuong Nguyen, Thu; Pham, Thi Nam; Phuong Vu, Thi; Tran, Dai Lam; Thai, Hoang; Thanh Dinh, Thi Mai

    2013-09-01

    In this paper, the synthesis of hydroxyapatite (HAp) nanopowder was studied by chemical precipitation method at different values of reaction temperature, settling time, Ca/P ratio, calcination temperature, (NH4)2HPO4 addition rate, initial concentration of Ca(NO3)2 and (NH4)2HPO4. Analysis results of properties, morphology, structure of HAp powder from infrared (IR) spectra, x-ray diffraction (XRD), energy dispersive x-ray (EDX) spectra and scanning electron microscopy (SEM) indicated that the synthesized HAp powder had cylinder crystal shape with size less than 100 nm, single-phase structure. The variation of the synthesis conditions did not affect the morphology but affected the size of HAp crystals.

  1. Simple synthetic route for hydroxyapatite colloidal nanoparticles via a Nd:YAG laser ablation in liquid medium

    NASA Astrophysics Data System (ADS)

    Mhin, Sung Wook; Ryu, Jeong Ho; Kim, Kang Min; Park, Gyeong Seon; Ryu, Han Wool; Shim, Kwang Bo; Sasaki, Takeshi; Koshizaki, Naoto

    2009-08-01

    Pulsed laser ablation (PLA) in liquid medium was successfully employed to synthesize hydroxyapatite (HAp) colloidal nanoparticles. The crystalline phase, particle morphology, size distribution and microstructure of the HAp nanoparticles were investigated in detail. The obtained HAp nanoparticles had spherical shape with sizes ranging from 5 to 20 nm. The laser ablation and the nanoparticle forming process were studied in terms of the explosive ejection mechanism by investigating the change of the surface morphology on target. The stoichiometry and bonding properties were studied by using XPS, FT-IR and Raman spectroscopy. A molar ratio of Ca/P of the prepared HAp nanoparticles was more stoichiometric than the value reported in the case of ablation in vacuum.

  2. Left-right asymmetries and shape analysis on Ceroglossus chilensis (Coleoptera: Carabidae)

    NASA Astrophysics Data System (ADS)

    Bravi, Raffaella; Benítez, Hugo A.

    2013-10-01

    Bilateral symmetry is widespread in animal kingdom, however most animal can deviate from expected symmetry and manifest some kind of asymmetries. Fluctuating asymmetry is considered as a tool for valuating developmental instability, whereas directional asymmetry is inherited and could be used for evaluating evolutionary development. We use the method of geometric morphometrics to analyze left/right asymmetries in the whole body, in two sites and totally six populations of Ceroglossus chilensis with the aim to infer and explain morphological disparities between populations and sexes in this species. In all individuals analyzed we found both fluctuating asymmetry and directional asymmetry for size and shape variation components, and a high sexual dimorphism. Moreover a high morphological variability between the two sites emerged as well. Differences in diet could influence the expression of morphological variation and simultaneously affect body sides, and therefore contribute to the symmetric component of variation. Moreover differences emerged between two sites could be a consequence of isolation and fragmentation, rather than a response to local environmental differences between sampling sites.

  3. Morphology of bone particles after harvesting with 4 different devices.

    PubMed

    Papadimitriou, Dimitrios E V; Schmidt, Erich C; Caton, Jack G; Romanos, Georgios E

    2013-04-01

    Autogenous bone is routinely used for regeneration of osseous defects around teeth and implants, and different instruments are available for bone harvesting. The purpose of this study was to describe the morphology of bone particles after harvesting with 4 different instruments. Bone particles were harvested from fresh cow ribs with 2 different types of back action chisels, a safescraper and a sonic device. The samples were examined morphologically using light microscopy and scanning electron microscopy. The bone particles after the back action chisel I had an appearance similar to "pencil shavings." With the back action chisel II, they were like thin paper with an "accordion bellows" appearance. After removal with the safescraper, they had an irregular shape (with an irregular surface) resembling "crushed stone." Finally, the appearance of the bone particles obtained with the sonic device was homogenous, condensed and continuous, and had a "seaweed" appearance. Harvesting of bone particles with 4 different devices produce distinctly difference sizes and shapes, which may influence the results of grafting procedures.

  4. Cellulose Nanocrystals vs. Cellulose Nanofibrils: A Comparative study on Their Microstructures and Effects as Polymer Reinforcing Agents

    Treesearch

    Xuezhu Xu; Fei Liu; Long Jiang; J.Y. Zhu; Darrin Haagenson; Dennis P. Wiesenborn

    2013-01-01

    Both cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNFs) are nanoscale cellulose fibers that have shown reinforcing effects in polymer nanocomposites. CNCs and CNFs are different in shape, size and composition. This study systematically compared their morphologies, crystalline structure, dispersion properties in polyethylene oxide (PEO) matrix, interactions...

  5. Confirmation of hybrid origin of Cyrtanthus based on the sequence analysis of internal transcribed spacer

    USDA-ARS?s Scientific Manuscript database

    The objectives of this study were to create interspecific hybrids between Cyrtanthus elatus and C. sanguineus and to confirm the hybrid origin of the progeny based on morphological characters and using molecular markers. The tip of the leaves, the shape and size of cells, and stomata distribution i...

  6. Two Echinostome Species, Pegosomum bubulcum and Nephrostomum ramosum (Digenea: Echinostomatidae), from an Eastern Cattle Egret, Bubulcus ibis coromandus, in Republic of Korea

    PubMed Central

    Choe, Seongjun; Lee, Dongmin; Park, Hansol; Jeon, Hyeong-Kyu; Lee, Youngsun; Kim, Eunju; Na, Ki-Jeong; Eom, Keeseon S.

    2016-01-01

    We describe 2 echinostome species recovered from an Eastern cattle egret, Bubulcus ibis coromandus, from Cheongju-si (city), Chungcheongbuk-do (province), Korea. Total 72 Pegosomum bubulcum specimens were recovered from the bile duct. They were 7,566×2,938 μm in average size and had 27 collar spines with vitelline extension from anterior 1/3 level of the esophagus to mid-level of the posterior testis as characteristic features. Total 9 specimens of Nephrostomum ramosum were recovered in the small intestines of the bird. They were ribbon-shaped, 11,378×2,124 μm in average size, and morphologically variable in some organs, i.e., the number of collar spines (47-50), the shape of ovary and testes, and the extension of vitelline follicles. These morphological variations observed in a single host indicated that these features are not critical for the classification of Nephrostomum species and thus were reconsidered taxonomically as synonym of N. ramosum. This study is the first report documenting and describing both flukes and their associated genera in Korea. PMID:27658601

  7. Two Echinostome Species, Pegosomum bubulcum and Nephrostomum ramosum (Digenea: Echinostomatidae), from an Eastern Cattle Egret, Bubulcus ibis coromandus, in Republic of Korea.

    PubMed

    Choe, Seongjun; Lee, Dongmin; Park, Hansol; Jeon, Hyeong-Kyu; Lee, Youngsun; Kim, Eunju; Na, Ki-Jeong; Eom, Keeseon S

    2016-08-01

    We describe 2 echinostome species recovered from an Eastern cattle egret, Bubulcus ibis coromandus, from Cheongju-si (city), Chungcheongbuk-do (province), Korea. Total 72 Pegosomum bubulcum specimens were recovered from the bile duct. They were 7,566×2,938 μm in average size and had 27 collar spines with vitelline extension from anterior 1/3 level of the esophagus to mid-level of the posterior testis as characteristic features. Total 9 specimens of Nephrostomum ramosum were recovered in the small intestines of the bird. They were ribbon-shaped, 11,378×2,124 μm in average size, and morphologically variable in some organs, i.e., the number of collar spines (47-50), the shape of ovary and testes, and the extension of vitelline follicles. These morphological variations observed in a single host indicated that these features are not critical for the classification of Nephrostomum species and thus were reconsidered taxonomically as synonym of N. ramosum. This study is the first report documenting and describing both flukes and their associated genera in Korea.

  8. Electron Transfer Strategies Regulate Carbonate Mineral and Micropore Formation

    NASA Astrophysics Data System (ADS)

    Zeng, Zhirui; Tice, Michael M.

    2018-01-01

    Some microbial carbonates are robust biosignatures due to their distinct morphologies and compositions. However, whether carbonates induced by microbial iron reduction have such features is unknown. Iron-reducing bacteria use various strategies to transfer electrons to iron oxide minerals (e.g., membrane-bound enzymes, soluble electron shuttles, nanowires, as well as different mechanisms for moving over or attaching to mineral surfaces). This diversity has the potential to create mineral biosignatures through manipulating the microenvironments in which carbonate precipitation occurs. We used Shewanella oneidensis MR-1, Geothrix fermentans, and Geobacter metallireducens GS-15, representing three different strategies, to reduce solid ferric hydroxide in order to evaluate their influence on carbonate and micropore formation (micro-size porosity in mineral rocks). Our results indicate that electron transfer strategies determined the morphology (rhombohedral, spherical, or long-chained) of precipitated calcium-rich siderite by controlling the level of carbonate saturation and the location of carbonate formation. Remarkably, electron transfer strategies also produced distinctive cell-shaped micropores in both carbonate and hydroxide minerals, thus producing suites of features that could potentially serve as biosignatures recording information about the sizes, shapes, and physiologies of iron-reducing organisms.

  9. Effect of the growth rate on the morphology and structural properties of hut-shaped Ge islands in Si(001).

    PubMed

    Yakimov, A I; Nikiforov, A I; Dvurechenskii, A V; Ulyanov, V V; Volodin, V A; Groetzschel, R

    2006-09-28

    The effect of Ge deposition rate on the morphology and structural properties of self-assembled Ge/Si(001) islands was studied. Ge/Si(001) layers were grown by solid-source molecular-beam epitaxy at 500 °C. We adjusted the Ge coverage, 6 monolayers (ML), and varied the Ge growth rate by a factor of 100, R = 0.02-2 ML s(-1), to produce films consisting of hut-shaped Ge islands. The samples were characterized by scanning tunnelling microscopy, Raman spectroscopy, and Rutherford backscattering measurements. The mean lateral size of Ge nanoclusters decreases from 14.1 nm at R = 0.02 ML s(-1) to 9.8 nm at R = 2 ML s(-1). The normalized width of the size distribution shows non-monotonic behaviour as a function of R and has a minimum value of 19% at R = 2 ML s(-1). Ge nanoclusters fabricated at the highest deposition rate demonstrate the best structural quality and the highest Ge content (∼0.9).

  10. New insights in morphological analysis for managing activated sludge systems.

    PubMed

    Oliveira, Pedro; Alliet, Marion; Coufort-Saudejaud, Carole; Frances, Christine

    2018-06-01

    In activated sludge (AS) process, the impact of the operational parameters on process efficiency is assumed to be correlated with the sludge properties. This study provides a better insight into these interactions by subjecting a laboratory-scale AS system to a sequence of operating condition modifications enabling typical situations of a wastewater treatment plant to be represented. Process performance was assessed and AS floc morphology (size, circularity, convexity, solidity and aspect ratio) was quantified by measuring 100,000 flocs per sample with an automated image analysis technique. Introducing 3D distributions, which combine morphological properties, allowed the identification of a filamentous bulking characterized by a floc population shift towards larger sizes and lower solidity and circularity values. Moreover, a washout phenomenon was characterized by smaller AS flocs and an increase in their solidity. Recycle ratio increase and COD:N ratio decrease both promoted a slight reduction of floc sizes and a constant evolution of circularity and convexity values. The analysis of the volume-based 3D distributions turned out to be a smart tool to combine size and shape data, allowing a deeper understanding of the dynamics of floc structure under process disturbances.

  11. Gold-Copper alloy “nano-dumplings” with tunable compositions and plasmonic properties

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Verma, Manoj, E-mail: duplasmonic@gmail.com; Kedia, Abhitosh; Kumar, P. Senthil

    The unique yet tunable optical properties of plasmonic metal nanoparticles have made them attractive targets for a wide range of applications including nanophotonics, molecular sensing, catalysis etc. Such diverse applications that require precisely stable / reproducible plasmonic properties depend sensitively on the particle morphology ie. the shape, size and constituents. Herein, we systematically study the size / shape controlled synthesis of gold-copper “dumpling” shaped alloy nanoparticles by simultaneous reduction of gold and copper salts in the PVP-methanol solute-solvent system, by effectively utilizing the efficient but mild reduction as well as capping abilities of Poly (N-vinylpyrrolidone). Introduction of copper salts notmore » only yielded the alloy nanoparticles, but also slowed down the growth process to maintain high mono-dispersity of the new shapes evolved. Copper and gold has different lattice constants (0.361 and 0.408 nm respectively) and hence doping/addition/replacement of copper atoms to gold FCC unit cell introduces strain into the lattice which is key parameter to the shape evolution in anisotropic nanoparticles. Synthesized alloy nanoparticles were characterized by UV-visible absorption spectroscopy, XRD and TEM imaging.« less

  12. Leaf Shape Responds to Temperature but Not CO2 in Acer rubrum

    PubMed Central

    Royer, Dana L.

    2012-01-01

    The degree of leaf dissection and the presence of leaf teeth, along with tooth size and abundance, inversely correlate with mean annual temperature (MAT) across many plant communities. These relationships form the core of several methods for reconstructing MAT from fossils, yet the direct selection of temperature on tooth morphology has not been demonstrated experimentally. It is also not known if atmospheric CO2 concentration affects leaf shape, limiting confidence in ancient climate reconstructions because CO2 has varied widely on geologic timescales. Here I report the results of growing Acer rubrum (red maple) in growth cabinets at contrasting temperature and CO2 conditions. The CO2 treatment imparted no significant differences in leaf size and shape, while plants grown at cooler temperatures tended to have more teeth and more highly dissected leaves. These results provide direct evidence for the selection of temperature on leaf shape in one species, and support a key link in many leaf-climate methods. More broadly, these results increase confidence for using leaf shape in fossils to reconstruct paleoclimate. PMID:23152921

  13. Gold-Copper alloy "nano-dumplings" with tunable compositions and plasmonic properties

    NASA Astrophysics Data System (ADS)

    Verma, Manoj; Kedia, Abhitosh; Kumar, P. Senthil

    2016-05-01

    The unique yet tunable optical properties of plasmonic metal nanoparticles have made them attractive targets for a wide range of applications including nanophotonics, molecular sensing, catalysis etc. Such diverse applications that require precisely stable / reproducible plasmonic properties depend sensitively on the particle morphology ie. the shape, size and constituents. Herein, we systematically study the size / shape controlled synthesis of gold-copper "dumpling" shaped alloy nanoparticles by simultaneous reduction of gold and copper salts in the PVP-methanol solute-solvent system, by effectively utilizing the efficient but mild reduction as well as capping abilities of Poly (N-vinylpyrrolidone). Introduction of copper salts not only yielded the alloy nanoparticles, but also slowed down the growth process to maintain high mono-dispersity of the new shapes evolved. Copper and gold has different lattice constants (0.361 and 0.408 nm respectively) and hence doping/addition/replacement of copper atoms to gold FCC unit cell introduces strain into the lattice which is key parameter to the shape evolution in anisotropic nanoparticles. Synthesized alloy nanoparticles were characterized by UV-visible absorption spectroscopy, XRD and TEM imaging.

  14. Subsurface synthesis and characterization of Ag nanoparticles embedded in MgO

    NASA Astrophysics Data System (ADS)

    Vilayurganapathy, S.; Devaraj, A.; Colby, R.; Pandey, A.; Varga, T.; Shutthanandan, V.; Manandhar, S.; El-Khoury, P. Z.; Kayani, Asghar; Hess, W. P.; Thevuthasan, S.

    2013-03-01

    Metal nanoparticles exhibit a localized surface plasmon resonance (LSPR) which is very sensitive to the size and shape of the nanoparticle and the surrounding dielectric medium. The coupling between the electromagnetic radiation and the localized surface plasmon in metallic nanoparticles results in a sizable enhancement of the incident fields, making them possible candidates for plasmonic applications. In particular, partially exposed metallic nanoparticles distributed in a dielectric matrix can provide prime locations for LSPR spectroscopy and sensing. We report the synthesis and characterization of a plasmonic substrate consisting of Ag nanoparticles partially buried in MgO. Ag nanoparticles of different shapes and size distributions were synthesized below the surface of MgO by implanting 200 keV Ag+ ions followed by annealing at 1000 °C for 10 and 30 h. A detailed optical and structural characterization was carried out to understand the evolution of the Ag nanoparticle and size distribution inside the MgO matrix. Micro x-ray diffraction (Micro-XRD) was employed to investigate the structural properties and estimate the crystallite size. The nanoparticles evolved from a spherical to a faceted morphology with annealing time, assuming an octahedral shape truncated at the (001) planes, as visualized from aberration-corrected transmission electron microscopy (TEM) images. The nanoparticles embedded in MgO were shown to be pure metallic Ag using atom probe tomography (APT). The nanoparticles were partially exposed to the surface by employing plasma etch techniques to remove the overlaying MgO. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were employed to study the surface morphology and obtain a height distribution for the partially exposed nanoparticles.

  15. 3-D Modelling of Megaloolithid Clutches: Insights about Nest Construction and Dinosaur Behaviour

    PubMed Central

    Vila, Bernat; Jackson, Frankie D.; Fortuny, Josep; Sellés, Albert G.; Galobart, Àngel

    2010-01-01

    Background Megaloolithid eggs have long been associated with sauropod dinosaurs. Despite their extensive and worldwide fossil record, interpretations of egg size and shape, clutch morphology, and incubation strategy vary. The Pinyes locality in the Upper Cretaceous Tremp Formation in the southern Pyrenees, Catalonia provides new information for addressing these issues. Nine horizons containing Megaloolithus siruguei clutches are exposed near the village of Coll de Nargó. Tectonic deformation in the study area strongly influenced egg size and shape, which could potentially lead to misinterpretation of reproductive biology if 2D and 3D maps are not corrected for bed dip that results from tectonism. Methodology/Findings Detailed taphonomic study and three-dimensional modelling of fossil eggs show that intact M. siruguei clutches contained 20–28 eggs, which is substantially larger than commonly reported from Europe and India. Linear and grouped eggs occur in three superimposed levels and form an asymmetric, elongate, bowl-shaped profile in lateral view. Computed tomography data support previous interpretations that the eggs hatched within the substrate. Megaloolithid clutch sizes reported from other European and Indian localities are typically less than 15 eggs; however, these clutches often include linear or grouped eggs that resemble those of the larger Pinyes clutches and may reflect preservation of incomplete clutches. Conclusions/Significance We propose that 25 eggs represent a typical megaloolithid clutch size and smaller egg clusters that display linear or grouped egg arrangements reported at Pinyes and other localities may represent eroded remnants of larger clutches. The similarity of megaloolithid clutch morphology from localities worldwide strongly suggests common reproductive behaviour. The distinct clutch geometry at Pinyes and other localities likely resulted from the asymmetrical, inclined, and laterally compressed titanosaur pes unguals of the female, using the hind foot for scratch-digging during nest excavation. PMID:20463953

  16. Puncture-and-Pull Biomechanics in the Teeth of Predatory Coelurosaurian Dinosaurs.

    PubMed

    Torices, Angelica; Wilkinson, Ryan; Arbour, Victoria M; Ruiz-Omeñaca, Jose Ignacio; Currie, Philip J

    2018-05-07

    The teeth of putatively carnivorous dinosaurs are often blade-shaped with well-defined serrated cutting edges (Figure 1). These ziphodont teeth are often easily differentiated based on the morphology and density of the denticles [1, 2]. A tearing function has been proposed for theropod denticles in general [3], but the functional significance of denticle phenotypic variation has received less attention. In particular, the unusual hooked denticles found in troodontids suggest a different feeding strategy or diet compared to other small theropods. We used a two-pronged approach to investigate the function of denticle shape variation across theropods with both congruent body shapes and sizes (e.g., dromaeosaurids versus troodontids) and highly disparate body shapes and sizes (e.g., troodontids versus tyrannosaurids), using microwear and finite element analyses (Figure 1). We found that many toothed coelurosaurian theropods employed a puncture-and-pull feeding movement, in which parallel scratches form while biting down into prey and oblique scratches form as the head is pulled backward with the jaws closed. In finite element simulations, theropod teeth had the lowest stresses when bite forces were aligned with the oblique family of microwear scratches. Different denticle morphologies performed differently under a variety of simulated biting angles: Dromaeosaurus and Saurornitholestes were well-adapted for handling struggling prey, whereas troodontid teeth were more likely to fail at non-optimal bite angles. Troodontids may have favored softer, smaller, or immobile prey. Copyright © 2018 Elsevier Ltd. All rights reserved.

  17. Surface characteristics of isopod digestive gland epithelium studied by SEM.

    PubMed

    Millaku, Agron; Leser, Vladka; Drobne, Damjana; Godec, Matjaz; Torkar, Matjaz; Jenko, Monika; Milani, Marziale; Tatti, Francesco

    2010-05-01

    The structure of the digestive gland epithelium of a terrestrial isopod Porcellio scaber has been investigated by conventional scanning electron microscopy (SEM), focused ion beam-scanning electron microscopy (FIB/SEM), and light microscopy in order to provide evidence on morphology of the gland epithelial surface in animals from a stock culture. We investigated the shape of cells, extrusion of lipid droplets, shape and distribution of microvilli, and the presence of bacteria on the cell surface. A total of 22 animals were investigated and we found some variability in the appearance of the gland epithelial surface. Seventeen of the animals had dome-shaped digestive gland "normal" epithelial cells, which were densely and homogeneously covered by microvilli and varying proportions of which extruded lipid droplets. On the surface of microvilli we routinely observed sparsely distributed bacteria of different shapes. Five of the 22 animals had "abnormal" epithelial cells with a significantly altered shape. In three of these animals, the cells were much smaller, partly or completely flat or sometimes pyramid-like. A thick layer of bacteria was detected on the microvillous border, and in places, the shape and size of microvilli were altered. In two animals, hypertrophic cells containing large vacuoles were observed indicating a characteristic intracellular infection. The potential of SEM in morphological investigations of epithelial surfaces is discussed.

  18. Absorption and emission spectroscopy of individual semiconductor nanostructures

    NASA Astrophysics Data System (ADS)

    McDonald, Matthew P.

    The advent of controllable synthetic methods for the production of semiconductor nanostructures has led to their use in a host of applications, including light-emitting diodes, field effect transistors, sensors, and even television displays. This is, in part, due to the size, shape, and morphologically dependent optical and electrical properties that make this class of materials extremely customizable; wire-, rod- and sphere-shaped nanocrystals are readily synthesized through common wet chemical methods. Most notably, confining the physical dimension of the nanostructure to a size below its Bohr radius (aB) results in quantum confinement effects that increase its optical energy gap. Not only the size, but the shape of a particle can be exploited to tailor its optical and electrical properties. For example, confined CdSe quantum dots (QDs) and nanowires (NWs) of equivalent diameter possess significantly different optical gaps. This phenomenon has been ascribed to electrostatic contributions arising from dielectric screening effects that are more pronounced in an elongated (wire-like) morphology. Semiconducting nanostructures have thus received significant attention over the past two decades. However, surprisingly little work has been done to elucidate their basic photophysics on a single particle basis. What has been done has generally been accomplished through emission-based measurements, and thus does not fully capture the full breadth of these intriguing systems. What is therefore needed then are absorption-based studies that probe the size and shape dependent evolution of nanostructure photophysics. This thesis summarizes the single particle absorption spectroscopy that we have carried out to fill this knowledge gap. Specifically, the diameter-dependent progression of one-dimensional (1D) excitonic states in CdSe NWs has been revealed. This is followed by a study that focuses on the polarization selection rules of 1D excitons within single CdSe NWs. Finally, shape effects are explored by probing the absorption spectra of CdSe nanowires and nanorods of varying length. All experimental studies are complemented by theoretical predictions from an effective mass model that takes electrostatic interactions into account. Thus, this thesis seeks to show the delicate interplay between quantum confinement and dielectric screening effects in single CdSe nanostructures.

  19. Cosmic dust synthesis by accretion and coagulation

    NASA Technical Reports Server (NTRS)

    Praburam, G.; Goree, J.

    1995-01-01

    The morphology of grains grown by accretion and coagulation is revaled by a new laboratory method of synthesizing cosmic dust analogs. Submicron carbon particles, grown by accretion of carbon atoms from a gas, have a spherical shape with a cauliflower-like surface and an internal micro-structure of radial columns. This shape is probably common for grains grown by accretion at a temperature well below the melting point. Coagulated grains, consisting of spheres that collided to form irregular strings, were also synthesized. Another shape we produced had a bumpy non- spherical morphology, like an interplanetary particle collected in the terrestrial stratosphere. Besides these isolated grains, large spongy aggregates of nanometer-size particles were also found for various experimental conditions. Grains were synthesized using ions to sputter a solid target, producing an atomic vapor at a low temperature. The ions were provided by a plasma, which also provided electrostatic levitation of the grains during their growth. The temporal development of grain growth was studied by extinguishing the plasma after various intervals.

  20. Effect of composition, morphology and size of nanozeolite on its in vitro cytotoxicity.

    PubMed

    Kihara, Takanori; Zhang, Yahong; Hu, Yuanyuan; Mao, Qiaofan; Tang, Yi; Miyake, Jun

    2011-06-01

    The extensive applications of nanoparticle materials in biomedical and biotechnological fields trigger the rapid development of nanotoxicology, because nanoparticles are reported to cause more damage than larger ones when human exposure to them. In the present manuscript, we prepared a series of zeolite nanocrystals with different frameworks, sizes, compositions and shapes, and provided the first report on their toxic difference. As our results, the toxicities of zeolite nanoparticles depend on their size, composition and shape when they are exposed to HeLa cells. The pure-silica nanozeolite silicalite-1 displays nontoxicity, but aluminum-containing nanozeolites, such as ZSM-5, LTL, and LTA, show a dose-dependent toxic manner. The different shapes of nanozeolites can lead to different cytotoxicities, while the influences of the surface charge differences of various nanozeolites on their toxicities are unconspicuous. More importantly, caspase-3 activity and LDH released assays showed that the toxic nanozeolites seem to induce cell necrosis rather than cell apoptosis by the damnification for the cell membranes. These results are expected to direct the applications of nanozeolites with different structures and shapes in biomedicine and clinic science. Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  1. Growth of elaborate microbial pinnacles in Lake Vanda, Antarctica.

    PubMed

    Sumner, D Y; Jungblut, A D; Hawes, I; Andersen, D T; Mackey, T J; Wall, K

    2016-11-01

    Microbial pinnacles in ice-covered Lake Vanda, McMurdo Dry Valleys, Antarctica, extend from the base of the ice to more than 50 m water depth. The distribution of microbial communities, their photosynthetic potential, and pinnacle morphology affects the local accumulation of biomass, which in turn shapes pinnacle morphology. This feedback, plus environmental stability, promotes the growth of elaborate microbial structures. In Lake Vanda, all mats sampled from greater than 10 m water depth contained pinnacles with a gradation in size from <1-mm-tall tufts to pinnacles that were centimeters tall. Small pinnacles were cuspate, whereas larger ones had variable morphology. The largest pinnacles were up to ~30 cm tall and had cylindrical bases and cuspate tops. Pinnacle biomass was dominated by cyanobacteria from the morphological and genomic groups Leptolyngbya, Phormidium, and Tychonema. The photosynthetic potential of these cyanobacterial communities was high to depths of several millimeters into the mat based on PAM fluorometry, and sufficient light for photosynthesis penetrated ~5 mm into pinnacles. The distribution of photosynthetic potential and its correlation to pinnacle morphology suggests a working model for pinnacle growth. First, small tufts initiate from random irregularities in prostrate mat. Some tufts grow into pinnacles over the course of ~3 years. As pinnacles increase in size and age, their interiors become colonized by a more diverse community of cyanobacteria with high photosynthetic potential. Biomass accumulation within this subsurface community causes pinnacles to swell, expanding laminae thickness and creating distinctive cylindrical bases and cuspate tops. This change in shape suggests that pinnacle morphology emerges from a specific distribution of biomass accumulation that depends on multiple microbial communities fixing carbon in different parts of pinnacles. Similarly, complex patterns of biomass accumulation may be reflected in the morphology of elaborate ancient stromatolites. © 2016 John Wiley & Sons Ltd.

  2. Ultrasonic assisted production of starch nanoparticles: Structural characterization and mechanism of disintegration.

    PubMed

    Boufi, Sami; Bel Haaj, Sihem; Magnin, Albert; Pignon, Frédéric; Impéror-Clerc, Marianne; Mortha, Gérard

    2018-03-01

    In this paper, the disintegration of starch (waxy and standard starch) granules into nanosized particles under the sole effect of high power ultrasonication treatment in water/isopropanol is investigated, by using wide methods of analysis. The present work aims at a fully characterization of the starch nanoparticles produced by ultrasonication, in terms of size, morphology and structural properties, and the proposition of a possible mechanism explaining the top-down generation of starch nanoparticles (SNPs) via high intensity ultrasonication. Dynamic light scattering measurements have indicated a leveling of the particle size to about 40nm after 75min of ultrasonication. The WAXD, DSC and Raman have revealed the amorphous character of the SNPs. FE-SEM. AFM observations have confirmed the size measured by DLS and suggested that SNPs exhibited 2D morphology of platelet-like shapes. This morphology is further supported by SAXS. On the basis of data collected from the different characterization techniques, a possible mechanism explaining the disintegration process of starch granules into NPs is proposed. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Effects of ornamentation and phylogeny on the evolution of wing shape in stalk-eyed flies (Diopsidae).

    PubMed

    Husak, J F; Ribak, G; Baker, R H; Rivera, G; Wilkinson, G S; Swallow, J G

    2013-06-01

    Exaggerated male ornaments are predicted to be costly to their bearers, but these negative effects may be offset by the correlated evolution of compensatory traits. However, when locomotor systems, such as wings in flying species, evolve to decrease such costs, it remains unclear whether functional changes across related species are achieved via the same morphological route or via alternate changes that have similar function. We conducted a comparative analysis of wing shape in relation to eye-stalk elongation across 24 species of stalk-eyed flies, using geometric morphometrics to determine how species with increased eye span, a sexually selected trait, have modified wing morphology as a compensatory mechanism. Using traditional and phylogenetically informed multivariate analyses of shape in combination with phenotypic trajectory analysis, we found a strong phylogenetic signal in wing shape. However, dimorphic species possessed shifted wing veins with the result of lengthening and narrowing wings compared to monomorphic species. Dimorphic species also had changes that seem unrelated to wing size, but instead may govern wing flexion. Nevertheless, the lack of a uniform, compensatory pattern suggests that stalk-eyed flies used alternative modifications in wing structure to increase wing area and aspect ratio, thus taking divergent morphological routes to compensate for exaggerated eye stalks. © 2013 The Authors. Journal of Evolutionary Biology © 2013 European Society For Evolutionary Biology.

  4. 3D Simulations of Void collapse in Energetic Materials

    NASA Astrophysics Data System (ADS)

    Rai, Nirmal Kumar; Udaykumar, H. S.

    2017-06-01

    Voids present in the microstructure of heterogeneous energetic materials effect the sensitivity towards ignition. It is established that the morphology of voids can play a significant role in sensitivity enhancement of energetic materials. Depending on the void shape, sensitivity can be either increased or decreased under given loading conditions. In the past, effects of different void shapes i.e. triangular, ellipse, cylindrical etc. on the sensitivity of energetic materials have been analyzed. However, most of these studies are performed in 2D and are limited under the plain strain assumption. Axisymmetric studies have also been performed in the past to incorporate the 3D effects, however axisymmetric modeling is limited to only certain geometries i.e. sphere. This work analyzes the effects of various void shapes in three dimensions on the ignition behavior of HMX. Various void shapes are analyzed including spherical, prolate and oblate speheroid oriented at different orientations, etc. Three dimensional void collapse simulations are performed on a single void to quantify the effects void morphology on initiation. A Cartesian grid based Eulerian solver SCIMITAR3D is used to perform the void collapse simulations. Various aspects of void morphology i.e. size, thickness of voids, elongation, orientation etc. are considered to obtain a comprehensive analysis. Also, 2D plane strain calculations are compared with the three dimensional analysis to evaluate the salient differences between 2D and 3D modeling.

  5. Endocranial Casts of Pre-Mammalian Therapsids Reveal an Unexpected Neurological Diversity at the Deep Evolutionary Root of Mammals.

    PubMed

    Benoit, Julien; Fernandez, Vincent; Manger, Paul R; Rubidge, Bruce S

    2017-01-01

    The origin and evolution of the mammalian brain has long been the focus of scientific enquiry. Conversely, little research has focused on the palaeoneurology of the stem group of Mammaliaformes, the Permian and Triassic non-mammaliaform Therapsida (NMT). This is because the majority of the NMT have a non-ossified braincase, making the study of their endocranial cast (sometimes called the "fossil brain") problematic. Thus, descriptions of the morphology and size of NMT endocranial casts have been based largely on approximations rather than reliable determination. Accordingly, here we use micro-CT scans of the skulls of 1 Dinocephalia and 3 Biarmosuchia, which are NMT with a fully ossified braincase and thus a complete endocast. For the first time, our work enables the accurate determination of endocranial shape and size in NMT. This study suggests that NMT brain size falls in the upper range of the reptilian and amphibian variation. Brain size in the dicynodont Kawingasaurus is equivalent to that of early Mammaliaformes, whereas the Dinocephalia show evidence of a secondary reduction of brain size. In addition, unlike other NMT in which the endocast has a tubular shape and its parts are arranged in a linear manner, the biarmosuchian endocast is strongly flexed at the level of the midbrain, creating a near right angle between the fore- and hindbrain. These data highlight an unexpected diversity of endocranial size and morphology in NMT, features that are usually considered conservative in this group. © 2017 S. Karger AG, Basel.

  6. Hearing capacities and otolith size in two ophidiiform species (Ophidion rochei and Carapus acus).

    PubMed

    Kéver, Loïc; Colleye, Orphal; Herrel, Anthony; Romans, Pascal; Parmentier, Eric

    2014-07-15

    Numerous studies have highlighted the diversity of fish inner ear morphology. However, the function of the shape, size and orientation of the different structures remains poorly understood. The saccule (otolithic endorgan) is considered to be the principal hearing organ in fishes and it has been hypothesized that sagitta (saccular otolith) shape and size affect hearing capacities: large sagittae are thought to increase sensitivity. The sagittae of many ophidiids and carapids occupy a large volume inside the neurocranium. Hence they are a good structure with which to test the size hypothesis. The main aim of this study was to investigate hearing capacities and inner ear morphology in two ophidiiform species: Ophidion rochei and Carapus acus. We used a multidisciplinary approach that combines dissections, μCT-scan examinations and auditory evoked potential techniques. Carapus acus and O. rochei sagittae have similar maximal diameters; both species have larger otoliths than many non-ophidiiform species, especially compared with the intra-neurocranium volume. Both species are sensitive to sounds up to 2100 Hz. Relative to the skull, O. rochei has smaller sagittae than the carapid, but better hearing capacities from 300 to 900 Hz and similar sensitivities at 150 Hz and from 1200 to 2100 Hz. Results show that hearing capacities of a fish species cannot be predicted only based on sagitta size. Larger otoliths (in size relative to the skull) may have evolved mainly for performing vestibular functions in fishes, especially those species that need to execute precise and complex movements. © 2014. Published by The Company of Biologists Ltd.

  7. [The comparative morphology of Trichinella spiralis and Trichinella pseudospiralis].

    PubMed

    Skvortsova, F K; Veretennikova, N L

    1997-01-01

    The comparative study of the morphology of invasive larvae and mature males and females T. spiralis and T. pseudospiralis at the head end of the parasites has revealed 14 papillae which are located in 3 circles and 2 which are placed bilaterally, 2 amphidae, and a lanceolar stiletto with a spicular cutting edge. There are no differences in the structure of the heads of these two Trichinella species. The larval cuticle is decorated only with cross folds while longitudinal small-sized folds which are arranged in regular rows appeared in mature species. The vulva in females is located at the levels of a fifth of the body from the head, it is slit-like and oval, that in T. pseudospiralis is situated at the cuticular projection. The anus in T. pseudospiralis larvae is as a cross oval slit, that in adult females is as a hemisphere, and in adult males it is coincident with the copulation pore. As for the structure of the pseudobursa, differences are found in the size and shape of copulation processes, in the arrangement of pre- and postanal papillae against the cloaca, in the size of the formed cuticular projections in T. spiralis, in the shape of the cloacal hole. In the unscrewed copulatory bell, it opens transversely in T. spiralis and vertically in T. pseudospiralis.

  8. Synthesis and characterization of nano-hydroxyapatite using Sapindus Mukorossi extract

    NASA Astrophysics Data System (ADS)

    Subha, B.; Prasath, P. Varun; Abinaya, R.; Kavitha, R. J.; Ravichandran, K.

    2015-06-01

    Nano-Hydroxyapatite (HAP) powders were successfully synthesised by hydrothermal method using Sapindus Mukorossi extract as an additive. The structural and morphological analyses of thus synthesised powders were carried out using FT-IR, XRD and FESEM/EDX. The FT-IR spectra confirm the presence of phosphate and hydroxyl groups corresponding to HAP. The XRD analysis reveals the formation of HAP phase and found to reduce the crystallite size with addition of Sapindus Mukorossi extract. The morphology changes from sphere to flake shape by the influence of extract.

  9. TMA Vessel Segmentation Based on Color and Morphological Features: Application to Angiogenesis Research

    PubMed Central

    Fernández-Carrobles, M. Milagro; Tadeo, Irene; Bueno, Gloria; Noguera, Rosa; Déniz, Oscar; Salido, Jesús; García-Rojo, Marcial

    2013-01-01

    Given that angiogenesis and lymphangiogenesis are strongly related to prognosis in neoplastic and other pathologies and that many methods exist that provide different results, we aim to construct a morphometric tool allowing us to measure different aspects of the shape and size of vascular vessels in a complete and accurate way. The developed tool presented is based on vessel closing which is an essential property to properly characterize the size and the shape of vascular and lymphatic vessels. The method is fast and accurate improving existing tools for angiogenesis analysis. The tool also improves the accuracy of vascular density measurements, since the set of endothelial cells forming a vessel is considered as a single object. PMID:24489494

  10. Size and diet in the evolution of African ape craniodental form.

    PubMed

    Shea, B T

    1983-01-01

    Interspecific differences in craniodental morphology among Pan paniscus, Pan troglodytes, and Gorilla gorilla are analyzed. These apes differ in both diet and body size, and thus present an excellent example in which to apply an allometric criterion of subtraction in order to determine morphological differences which might be related to divergent dietary specialization. The use of ontogenetic allometry in particular as a criterion of subtraction is discussed. Bivariate and multivariate results indicate that most of the variation in skull form among the species relates to the extension of a common growth trend to different sizes. Comparative analysis of growth trajectories reveals a number of differences, but none that appear to relate to a reorganization of skull proportions which might correspond to a dietary shift towards increased folivory. The dentition clearly exhibits non-allometric shape changes corresponding to the dietary differences, however. The meaning of these differences between cranial and dental patterns is discussed.

  11. Geometric morphometric analysis of allometric variation in the mandibular morphology of the hominids of Atapuerca, Sima de los Huesos site.

    PubMed

    Rosas, Antonio; Bastir, Markus

    2004-06-01

    Allometry is an important factor of morphological integration that contributes to the organization of the phenotype and its variation. Variation in the allometric shape of the mandible is particularly important in hominid evolution because the mandible carries important taxonomic traits. Some of these traits are known to covary with size, particularly the retromolar space, symphyseal curvature, and position of the mental foramen. The mandible is a well studied system in the context of the evolutionary development of complex morphological structures because it is composed of different developmental units that are integrated within a single bone. In the present study, we investigated the allometric variation of two important developmental units that are separated by the inferior nerve (a branch of CN V3). We tested the null hypothesis that there would be no difference in allometric variation between the two components. Procrustes-based geometric morphometrics of 20 two-dimensional (2D) landmarks were analyzed by multivariate regressions of shape on size in samples from 121 humans, 48 chimpanzees, and 50 gorillas (all recent specimens), eight fossil hominids from Atapuerca, Sima de los Huesos (AT-SH), and 17 Neandertals. The findings show that in all of the examined species, there was significantly greater allometric variation in the supra-nerve unit than in the infra-nerve unit. The formation of the retromolar space exhibited an allometric relationship with the supra-nerve unit in all of the species studied. The formation of the chin-like morphology is an "apodynamic" feature of the infra-nerve unit in the AT-SH hominids. The results of this study support the hypothesis that allometry contributes to the organization of variation in complex morphological structures. Copyright 2004 Wiley-Liss, Inc.

  12. Fabrication of hexagonal star-shaped and ring-shaped patterns arrays by Mie resonance sphere-lens-lithography

    NASA Astrophysics Data System (ADS)

    Liu, Xianchao; Wang, Jun; Li, Ling; Gou, Jun; Zheng, Jie; Huang, Zehua; Pan, Rui

    2018-05-01

    Mie resonance sphere-lens-lithography has proved to be a good candidate for fabrication of large-area tunable surface nanopattern arrays. Different patterns on photoresist surface are obtained theoretically by adjusting optical coupling among neighboring spheres with different gap sizes. The effect of light reflection from the substrate on the pattern produced on the photoresist with a thin thickness is also discussed. Sub-micron hexagonal star-shaped and ring-shaped patterns arrays are achieved with close-packed spheres arrays and spheres arrays with big gaps, respectively. Changing of star-shaped vertices is induced by different polarization of illumination. Experimental results agree well with the simulation. By using smaller resonance spheres, sub-400 nm star-shaped and ring-shaped patterns can be realized. These tunable patterns are different from results of previous reports and have enriched pattern morphology fabricated by sphere-lens-lithography, which can find application in biosensor and optic devices.

  13. Intrapopulation variation in stature and body proportions: social status and sex differences in an Italian medieval population (Trino Vercellese, VC).

    PubMed

    Vercellotti, Giuseppe; Stout, Sam D; Boano, Rosa; Sciulli, Paul W

    2011-06-01

    The phenotypic expression of adult body size and shape results from synergistic interactions between hereditary factors and environmental conditions experienced during growth. Variation in body size and shape occurs even in genetically relatively homogeneous groups, due to different occurrence, duration, and timing of growth insults. Understanding the causes and patterns of intrapopulation variation can foster meaningful information on early life conditions in living and past populations. This study assesses the pattern of biological variation in body size and shape attributable to sex and social status in a medieval Italian population. The sample includes 52 (20 female, 32 male) adult individuals from the medieval population of Trino Vercellese, Italy. Differences in element size and overall body size (skeletal height and body mass) were assessed through Monte Carlo methods, while univariate non-parametric tests and Principal Component Analysis (PCA) were employed to examine segmental and overall body proportions. Discriminant Analysis was employed to determine the predictive value of individual skeletal elements for social status in the population. Our results highlight a distinct pattern in body size and shape variation in relation to status and sex. Male subsamples exhibit significant postcranial variation in body size, while female subsamples express smaller, nonsignificant differences. The analysis of segmental proportions highlighted differences in trunk/lower limb proportions between different status samples, and PCA indicated that in terms of purely morphological variation high status males were distinct from all other groups. The pattern observed likely resulted from a combination of biological factors and cultural practices. Copyright © 2011 Wiley-Liss, Inc.

  14. Probing defects in ZnO nanostructures by Photoluminescence and Positron Annihilation Spectroscopy

    NASA Astrophysics Data System (ADS)

    Ghosh, Manoranjan; Raychaudhuri, A. K.; Chaudhuri, S. K.; Das, Dipankar

    2008-03-01

    We have investigated defect related emission in the blue green region (2.2 eV -- 2.5 eV) of ZnO nanostructures having spherical (5 nm-15 nm) as well as those with hexagonal platelet and rod like morphologies (20nm-100 nm), synthesized by solvo-thermal route. This emission show anomalous size dependence. Emission energy near 2.2 eV, shifts to higher energy (2.5 eV) for increase in size beyond 20nm when shape of the nanostructures changes. This change in photoluminescence has a close correlation with the size (and shape) induced change in the positron trapping rate which is directly proportional to the defect concentration. The trapping rates show non-monotonous dependence on size. It increases initially as the size increases (5nm-15nm) and then decreases as the size increases beyond 20nm. While increase of the trapping rate on size reduction is expected due to accumulation of more defects at the surface, the initial dependence of the trapping rate on the size (below 20nm) is anomalous. The data are explained by the presence of defects like Zn vacancy and confinement due to size reduction.

  15. Preparation and characterization of Ba0.2Sr0.2La0.6MnO3 nanoparticles and investigation of size & shape effect on microwave absorption

    NASA Astrophysics Data System (ADS)

    Peymanfar, Reza; Javanshir, Shahrzad

    2017-06-01

    In this paper, the design and characterization of a radar absorbing material (RAM) was investigated at microwave frequency. Ba0.2Sr0.2La0.6MnO3 magnetic nanoparticles was synthesized thru a facile hydrothermal method in the presence of polymethyl methacrylate (PMMA) and the possibility of shape and size-controlled synthesis of nanoparticles (NPs) over the range 15-50 Nm was also explored. Afterward, the effect of shape and size of the synthesized Ba0.2Sr0.2La0.6MnO3 NPs on microwave absorption properties was investigated in KU-band. The crystal structures and morphology of as-synthesized nanoparticles were characterized and confirmed by FESEM, XRD, VSM, FTIR analysis. The RAM samples were prepared by dispersion of magnetic NPs in silicone rubber in an ultrasonic bath. The maximum reflection loss (RL) values NPs were 12.04 dB at 14.82 GHz and a broad absorption band (over 1.22 GHz) with RL values <-10 dB are obtained and the maximum reflection loss (RL) values of decrease and shaped NPs were 22.36 dB at 14.78 GHz and a broad absorption band (over 2.67 GHz) with RL values <-10 dB are obtained. The results indicated that the particle size and shape play a major role on the absorption properties of the composites in the 12.4-18 GHz frequency range. It is observed that microwave absorption properties increased with the decrease in average particle size of NPs.

  16. Dislocation, crystallite size distribution and lattice strain of magnesium oxide nanoparticles

    NASA Astrophysics Data System (ADS)

    Sutapa, I. W.; Wahid Wahab, Abdul; Taba, P.; Nafie, N. L.

    2018-03-01

    The oxide of magnesium nanoparticles synthesized using sol-gel method and analysis of the structural properties was conducted. The functional groups of nanoparticles has been analysed by Fourier Transform Infrared Spectroscopy (FT-IR). Dislocations, average size of crystal, strain, stress, the energy density of crystal, crystallite size distribution and morphologies of the crystals were determined based on X-ray diffraction profile analysis. The morphological of the crystal was analysed based on the image resulted from SEM analysis. The crystallite size distribution was calculated with the contention that the particle size has a normal logarithmic form. The most orientations of crystal were determined based on the textural crystal from diffraction data of X-ray diffraction profile analysis. FT-IR results showed the stretching vibration mode of the Mg-O-Mg in the range of 400.11-525 cm-1 as a broad band. The average size crystal of nanoparticles resulted is 9.21 mm with dislocation value of crystal is 0.012 nm-2. The strains, stress, the energy density of crystal are 1.5 x 10-4 37.31 MPa; 0.72 MPa respectively. The highest texture coefficient value of the crystal is 0.98. This result is supported by morphological analysis using SEM which shows most of the regular cubic-shaped crystals. The synthesis method is suitable for simple and cost-effective synthesis model of MgO nanoparticles.

  17. Electrosynthesis and characterization of zinc tungstate nanoparticles

    NASA Astrophysics Data System (ADS)

    Rahimi-Nasrabadi, Mehdi; Pourmortazavi, Seied Mahdi; Ganjali, Mohammad Reza; Hajimirsadeghi, Seiedeh Somayyeh; Zahedi, Mir Mahdi

    2013-09-01

    Zinc tungstate nanoparticles with different sizes are produced through an electrolysis process including a zinc plate anode in sodium tungstate solution. The shape and size of the product was found to be controlled by varying reaction parameters such as electrolysis voltage, stirring rate of electrolyte solution and temperature. The morphological (SEM) characterization analysis was performed on the product and UV-Vis spectrophotometry and FT-IR spectroscopy was utilized to characterize the electrodeposited nanoparticles. Study of the particle size of the product versus the electrolysis voltage showed that, increasing the voltage from 4 to 8 V, led to the particle size of zinc tungstate to decrease, but further increasing the voltage from 8 to 12 V, the particle size of the produced particles increased. The size and shape of the product was also found to be dependent on the stirring rate and temperature of the electrolyte solution. X-ray diffraction (XRD), scanning electron microscopy (SEM), FT-IR spectroscopy, and photoluminescence, were used to study the structure as well as composition of the nano-material prepared under optimum conditions.

  18. A thin-plate spline analysis of the face and tongue in obstructive sleep apnea patients.

    PubMed

    Pae, E K; Lowe, A A; Fleetham, J A

    1997-12-01

    The shape characteristics of the face and tongue in obstructive sleep apnea (OSA) patients were investigated using thin-plate (TP) splines. A relatively new analytic tool, the TP spline method, provides a means of size normalization and image analysis. When shape is one's main concern, various sizes of a biologic structure may be a source of statistical noise. More seriously, the strong size effect could mask underlying, actual attributes of the disease. A set of size normalized data in the form of coordinates was generated from cephalograms of 80 male subjects. The TP spline method envisioned the differences in the shape of the face and tongue between OSA patients and nonapneic subjects and those between the upright and supine body positions. In accordance with OSA severity, the hyoid bone and the submental region positioned inferiorly and the fourth vertebra relocated posteriorly with respect to the mandible. This caused a fanlike configuration of the lower part of the face and neck in the sagittal plane in both upright and supine body positions. TP splines revealed tongue deformations caused by a body position change. Overall, the new morphometric tool adopted here was found to be viable in the analysis of morphologic changes.

  19. Optimization of surface morphology and scattering properties of TCO/AIT textured glass front electrode for thin film solar cells

    NASA Astrophysics Data System (ADS)

    Addonizio, M. L.; Fusco, L.; Antonaia, A.; Cominale, F.; Usatii, I.

    2015-12-01

    Aluminium induced texture (AIT) method has been used for obtaining highly textured glass substrate suitable for silicon based thin film solar cell technology. Wet etch step parameters of AIT process have been varied and effect of different etchants and different etching times on morphological and optical properties has been analyzed. The resulting morphology features (shape, size distribution, inclination angle) have been optimized in order to obtain the best scattering properties. ZnO:Ga (GZO) films have been deposited by sputtering technique on AIT-processed glass. Two different ZnO surface morphologies have been obtained, strongly depending on the underlying glass substrate morphology induced by different etching times. Very rough and porous texture (σrms ∼ 150 nm) was obtained on glass etched 2 min showing cauliflower-like structure, whereas a softer texture (σrms ∼ 78 nm) was obtained on glass etched 7 min giving wider and smoother U-shaped craters. The effect of different glass textures on optical confinement has been tested in amorphous silicon based p-i-n devices. Devices fabricated on GZO/high textured glass showed a quantum efficiency enhancement due to both an effective light trapping phenomenon and an effective anti-reflective optical behaviour. Short etching time produce smaller cavities (<1 μm) with deep U-shape characterized by high roughness, high inclination angle and low autocorrelation length. This surface morphology promoted a large light scattering phenomenon, as evidenced by haze value and by angular resolved scattering (ARS) behaviour, into a large range of diffraction angles, giving high probability of effective light trapping inside a PV device.

  20. Host Diet Affects the Morphology of Monarch Butterfly Parasites.

    PubMed

    Hoang, Kevin; Tao, Leiling; Hunter, Mark D; de Roode, Jacobus C

    2017-06-01

    Understanding host-parasite interactions is essential for ecological research, wildlife conservation, and health management. While most studies focus on numerical traits of parasite groups, such as changes in parasite load, less focus is placed on the traits of individual parasites such as parasite size and shape (parasite morphology). Parasite morphology has significant effects on parasite fitness such as initial colonization of hosts, avoidance of host immune defenses, and the availability of resources for parasite replication. As such, understanding factors that affect parasite morphology is important in predicting the consequences of host-parasite interactions. Here, we studied how host diet affected the spore morphology of a protozoan parasite ( Ophryocystis elektroscirrha ), a specialist parasite of the monarch butterfly ( Danaus plexippus ). We found that different host plant species (milkweeds; Asclepias spp.) significantly affected parasite spore size. Previous studies have found that cardenolides, secondary chemicals in host plants of monarchs, can reduce parasite loads and increase the lifespan of infected butterflies. Adding to this benefit of high cardenolide milkweeds, we found that infected monarchs reared on milkweeds of higher cardenolide concentrations yielded smaller parasites, a potentially hidden characteristic of cardenolides that may have important implications for monarch-parasite interactions.

  1. The Comparative Osteology of the Petrotympanic Complex (Ear Region) of Extant Baleen Whales (Cetacea: Mysticeti)

    PubMed Central

    Ekdale, Eric G.; Berta, Annalisa; Deméré, Thomas A.

    2011-01-01

    Background Anatomical comparisons of the ear region of baleen whales (Mysticeti) are provided through detailed osteological descriptions and high-resolution photographs of the petrotympanic complex (tympanic bulla and petrosal bone) of all extant species of mysticete cetaceans. Salient morphological features are illustrated and identified, including overall shape of the bulla, size of the conical process of the bulla, morphology of the promontorium, and the size and shape of the anterior process of the petrosal. We place our comparative osteological observations into a phylogenetic context in order to initiate an exploration into petrotympanic evolution within Mysticeti. Principal Findings The morphology of the petrotympanic complex is diagnostic for individual species of baleen whale (e.g., sigmoid and conical processes positioned at midline of bulla in Balaenoptera musculus; confluence of fenestra cochleae and perilymphatic foramen in Eschrichtius robustus), and several mysticete clades are united by derived characteristics. Balaenids and neobalaenids share derived features of the bulla, such as a rhomboid shape and a reduced anterior lobe (swelling) in ventral aspect, and eschrichtiids share derived morphologies of the petrosal with balaenopterids, including loss of a medial promontory groove and dorsomedial elongation of the promontorium. Monophyly of Balaenoidea (Balaenidae and Neobalaenidae) and Balaenopteroidea (Balaenopteridae and Eschrichtiidae) was recovered in phylogenetic analyses utilizing data exclusively from the petrotympanic complex. Significance This study fills a major gap in our knowledge of the complex structures of the mysticete petrotympanic complex, which is an important anatomical region for the interpretation of the evolutionary history of mammals. In addition, we introduce a novel body of phylogenetically informative characters from the ear region of mysticetes. Our detailed anatomical descriptions, illustrations, and comparisons provide valuable data for current and future studies on the phylogenetic relationships, evolution, and auditory physiology of mysticetes and other cetaceans throughout Earth's history. PMID:21731700

  2. Multicomponent inorganic Janus particles with controlled compositions, morphologies, and dimensions.

    PubMed

    Lyubarskaya, Yekaterina L; Shestopalov, Alexander A

    2013-08-14

    We report a new protocol for the preparation of shape-controlled multicomponent particles comprising metallic (Au and Ti), magnetic (Ni), and oxide (SiO2, TiO2) layers. Our method allows for a precise control over the composition, shape, and size and permits fabrication of nonsymmetrical particles, whose opposite sides can be orthogonally functionalized using well-established organosilanes and thiol chemistries. Because of their unique geometries and surface chemistries, these colloids represent ideal materials with which to study nonsymmetrical self-assembly at the meso- and microscales.

  3. Morphological and Crystalline Transitions in Monohydrous and Anhydrous Aripiprazole for a Long-Acting Injectable Suspension.

    PubMed

    Tan, Xinyi; Zhong, Yue; He, Luying; Zhang, Yuanyuan; Jing, Guanghui; Li, Song; Wang, Jing; He, Haibing; Tang, Xing

    2017-05-01

    Many formulation and manufacturing processes can lead to morphological and crystalline transitions in many polycrystalline drugs, changing the properties of active pharmaceutical ingredients (APIs) such as solubility and physical stability which influence their therapeutic effects and safety and so limit their usefulness. Here, we report significant changes in crystal forms and morphology, including the shape and size of particles during the manufacture of off-white aripiprazole (APZ) dry powders used for long-acting and injectable suspensions. With the optimal top-down approach, powders were prepared by recrystallizing uniform monohydrous APZ (MA) and polycrystalline anhydrous APZ (AA) form III, characterized by thermal analysis, PXRD, and FT-IR. However, powders involving MA (MAP) with a lower mean size (2.126 μm), narrower distribution (span = 1.90), and higher stability compared with AA dry powders (AAP) were found to exhibit dehydration behavior and morphological changes after completion of the preparation processes based on the results of thermal analysis. In the case of APZ powders, we wished to obtain more information to guide in the industrial production and experimental design of suspensions in the future.

  4. Morphometry of network and nonnetwork space of basins

    NASA Astrophysics Data System (ADS)

    Chockalingam, L.; Daya Sagar, B. S.

    2005-08-01

    Morphometric analysis of channel network of a basin provides several scale- independent measures. To better characterize basin morphology, one requires, besides channel morphometric properties, scale-independent but shape-dependent measures to record the sensitive differences in the morphological organization of nonnetwork spaces. These spaces are planar forms of hillslopes or the retained portion after subtracting the channel network from the basin space. The principal aim of this paper is to focus on explaining the importance of alternative scale-independent but shape-dependent measures of nonnetwork spaces of basins. Toward this goal, we explore how mathematical morphology-based decomposition procedures can be used to derive basic measures required to quantify estimates, such as dimensionless power laws, that are useful to express the importance of characteristics of nonnetwork spaces via decomposition rules. We demonstrate our results through characterization of nonnetwork spaces of eight subbasins of the Gunung Ledang region of peninsular Malaysia. We decompose the nonnetwork spaces of eight fourth-order basins in a two-dimensional discrete space into simple nonoverlapping disks (NODs) of various sizes by employing morphological transformations. Furthermore, we show relationships between the dimensions estimated via morphometries of the network and their corresponding nonnetwork spaces. This study can be extended to characterize hillslope morphologies, where decomposition of three-dimensional hillslopes needs to be addressed.

  5. Electrum, the Gold-Silver Alloy, from the Bulk Scale to the Nanoscale: Synthesis, Properties, and Segregation Rules.

    PubMed

    Guisbiers, Grégory; Mendoza-Cruz, Rubén; Bazán-Díaz, Lourdes; Velázquez-Salazar, J Jesús; Mendoza-Perez, Rafael; Robledo-Torres, José Antonio; Rodriguez-Lopez, José-Luis; Montejano-Carrizales, Juan Martín; Whetten, Robert L; José-Yacamán, Miguel

    2016-01-26

    The alloy Au-Ag system is an important noble bimetallic phase, both historically (as "Electrum") and now especially in nanotechnology, as it is applied in catalysis and nanomedicine. To comprehend the structural characteristics and the thermodynamic stability of this alloy, a knowledge of its phase diagram is required that considers explicitly its size and shape (morphology) dependence. However, as the experimental determination remains quite challenging at the nanoscale, theoretical guidance can provide significant advantages. Using a regular solution model within a nanothermodynamic approach to evaluate the size effect on all the parameters (melting temperature, melting enthalpy, and interaction parameters in both phases), the nanophase diagram is predicted. Besides an overall shift downward, there is a "tilting" effect on the solidus-liquidus curves for some particular shapes exposing the (100) and (110) facets (cube, rhombic dodecahedron, and cuboctahedron). The segregation calculation reveals the preferential presence of silver at the surface for all the polyhedral shapes considered, in excellent agreement with the latest transmission electron microscopy observations and energy dispersive spectroscopy analysis. By reviewing the nature of the surface segregated element of different bimetallic nanoalloys, two surface segregation rules, based on the melting temperatures and surface energies, are deduced. Finally, the optical properties of Au-Ag nanoparticles, calculated within the discrete dipole approximation, show the control that can be achieved in the tuning of the local surface plasmon resonance, depending of the alloy content, the chemical ordering, the morphology, the size of the nanoparticle, and the nature of the surrounding environment.

  6. Scaling of size distributions of C60 and C70 fullerene surface islands

    NASA Astrophysics Data System (ADS)

    Dubrovskii, V. G.; Berdnikov, Y.; Olyanich, D. A.; Mararov, V. V.; Utas, T. V.; Zotov, A. V.; Saranin, A. A.

    2017-06-01

    We present experimental data and a theoretical analysis for the size distributions of C60 and C70 surface islands deposited onto In-modified Si(111)√3 × √3-Au surface under different conditions. We show that both fullerene islands feature an analytic Vicsek-Family scaling shape where the scaled size distributions are given by a power law times an incomplete beta-function with the required normalization. The power exponent in this distribution corresponds to the fractal shape of two-dimensional islands, confirmed by the experimentally observed morphologies. Quite interestingly, we do not see any significant difference between C60 and C70 fullerenes in terms of either scaling parameters or temperature dependence of the diffusion constants. In particular, we deduce the activation energy for surface diffusion of ED = 140 ± 10 meV for both types of fullerenes.

  7. Analysis of Surstylus and Aculeus Shape and Size Using Geometric Morphometrics to Discriminate Rhagoletis pomonella and Rhagoletis zephyria (Diptera: Tephritidae)

    USDA-ARS?s Scientific Manuscript database

    Rhagoletis pomonella (Walsh) and Rhagoletis zephyria Snow both occur in the Pacific Northwest of the U. S. and are frequently confused with one another due to their morphological similarity. The apple maggot, R. pomonella, is a threat to commercial apples in the Pacific Northwest, whereas R. zephyr...

  8. Synthesis of gold nanostructures using fruit extract of Garcinia Indica

    NASA Astrophysics Data System (ADS)

    Krishnaprabha, M.; Pattabi, Manjunatha

    2016-05-01

    Gold nanoparticles having different shapes are synthesized using extract of fresh fruit rinds of Garcinia Indica. The onset of growth and formation of gold nanostructures is confirmed from UV-Vis spectroscopy. Morphological studies are done using FESEM. Size dependent catalytic activity is evaluated with the model reduction reaction of 4-nitrophenol to 4-aminophenol.

  9. Dwarfs in the Deepest Fields at Noon: Studying Size and Shape of Low-mass Galaxies out to z 3 in Five HST Legacy Fields

    NASA Astrophysics Data System (ADS)

    Guo, Yicheng

    2017-08-01

    Galaxies with stellar mass 100x-1000x times smaller than our Milky Way (hereafter dwarf galaxies or DGs) are important for understanding galaxy formation and evolution by being the most sensitive probes of both the macro-physics of dark matter halos and the micro-physics of the different physical mechanisms that regulate star formation and shape galaxies. Currently, however, observations of distant DGs have been hampered by small samples and poor quality due to their faintness. We propose an archival study of the size, morphology, and structures of DGs out to z 3.0 by combining the archived data from five of the deepest regions that HST has ever observed: eXtreme Deep Field (XDF, updated from HUDF) and the Hubble Legacy Fields (HLFs). Our program would be the first to advance the morphology studies of DGs to the Cosmic Noon (z 2), and hence place unprecedented constraints on models of galaxy structure formation. Equally important is the data product of our program: multi-wavelength photometry and morphology catalogs for all detected galaxies in these fields. These catalogs would be a timely treasure for the public to prepare for the coming JWST era by providing detailed information of small, faint, but important objects in some deepest HST fields for JWST observations.

  10. Morphology and ontogenesis of Psilotrichides hawaiiensis nov. gen., nov. spec. and molecular phylogeny of the Psilotrichidae (Ciliophora, Hypotrichia).

    PubMed

    Heber, Domingo; Stoeck, Thorsten; Foissner, Wilhelm

    2014-01-01

    The Psilotrichidae are a family of middle-sized hypotrichs with unique morphological and ontogenetic features (e.g. the oral primordium develops in a deep pouch) that, however, did not provide a definite phylogenetic signal. Thus, we studied the 18S rRNA gene of Urospinula succisa (Müller 1786) Esteban et al., 2001 as well as the morphology and ontogenesis of Psilotrichides hawaiiensis, a new genus and species from an ephemeral swamp on Oahu Island, Hawaii. The molecular data classify the psilotrichids into the oxytrichids but without clear branching position. A brief revision, using the structure of the oral apparatus, the location of the contractile vacuole, and three ontogenetic features, showed four distinct genera: Psilotricha Stein, 1859; Urospinula Corliss, 1960; Hemiholosticha Gelei, 1954; and Psilotrichides nov. gen., which differs from the confamilials mainly by the obliquely oriented buccal cavity and the shape of the undulating membranes as well as by a distinct ridge along the right buccal margin. The pyriform species, P. hawaiiensis, is about 65 × 45 μm in size and is easily recognized by the table tennis racket-shaped appearance due to the elongated last cirrus of the left marginal row. Refined diagnoses are provided for the family Psilotrichidae Bütschli, 1889 and the genera contained. © 2014 The Author(s) Journal of Eukaryotic Microbiology © 2014 International Society of Protistologists.

  11. Stony Endocarp Dimension and Shape Variation in Prunus Section Prunus

    PubMed Central

    Depypere, Leander; Chaerle, Peter; Mijnsbrugge, Kristine Vander; Goetghebeur, Paul

    2007-01-01

    Background and Aims Identification of Prunus groups at subspecies or variety level is complicated by the wide range of variation and morphological transitional states. Knowledge of the degree of variability within and between species is a sine qua non for taxonomists. Here, a detailed study of endocarp dimension and shape variation for taxa of Prunus section Prunus is presented. Method The sample size necessary to obtain an estimation of the population mean with a precision of 5 % was determined by iteration. Two cases were considered: (1) the population represents an individual; and (2) the population represents a species. The intra-individual and intraspecific variation of Prunus endocarps was studied by analysing the coefficients of variance for dimension and shape parameters. Morphological variation among taxa was assessed using univariate statistics. The influence of the time of sampling and the level of hydration on endocarp dimensions and shape was examined by means of pairwise t-tests. In total, 14 endocarp characters were examined for five Eurasian plum taxa. Key Results All linear measurements and index values showed a low or normal variability on the individual and species level. In contrast, the parameter ‘Vertical Asymmetry’ had high coefficients of variance for one or more of the taxa studied. Of all dimension and shape parameters studied, only ‘Triangle’ differed significantly between mature endocarps of P. insititia sampled with a time difference of 1 month. The level of hydration affected endocarp dimensions and shape significantly. Conclusions Index values and the parameters ‘Perimeter’, ‘Area’, ‘Triangle’, ‘Ellipse’, ‘Circular’ and ‘Rectangular’, based on sample sizes and coefficients of variance, were found to be most appropriate for further taxonomic analysis. However, use of one, single endocarp parameter is not satisfactory for discrimination between Eurasian plum taxa, mainly because of overlapping ranges. Before analysing dried endocarps, full hydration is recommended, as this restores the original dimensions and shape. PMID:17965026

  12. A scattering database of marine particles and its application in optical analysis

    NASA Astrophysics Data System (ADS)

    Xu, G.; Yang, P.; Kattawar, G.; Zhang, X.

    2016-12-01

    In modeling the scattering properties of marine particles (e.g. phytoplankton), the laboratory studies imply a need to properly account for the influence of particle morphology, in addition to size and composition. In this study, a marine particle scattering database is constructed using a collection of distorted hexahedral shapes. Specifically, the scattering properties of each size bin and refractive index are obtained by the ensemble average associated with distorted hexahedra with randomly tilted facets and selected aspect ratios (from elongated to flattened). The randomness degree in shape-generation process defines the geometric irregularity of the particles in the group. The geometric irregularity and particle aspect ratios constitute a set of "shape factors" to be accounted for (e.g. in best-fit analysis). To cover most of the marine particle size range, we combine the Invariant Imbedding T-matrix (II-TM) method and the Physical-Geometric Optics Hybrid (PGOH) method in the calculations. The simulated optical properties are shown and compared with those obtained from Lorenz-Mie Theory. Using the scattering database, we present a preliminary optical analysis of laboratory-measured optical properties of marine particles.

  13. Effective density and morphology of particles emitted from small-scale combustion of various wood fuels.

    PubMed

    Leskinen, Jani; Ihalainen, Mika; Torvela, Tiina; Kortelainen, Miika; Lamberg, Heikki; Tiitta, Petri; Jakobi, Gert; Grigonyte, Julija; Joutsensaari, Jorma; Sippula, Olli; Tissari, Jarkko; Virtanen, Annele; Zimmermann, Ralf; Jokiniemi, Jorma

    2014-11-18

    The effective density of fine particles emitted from small-scale wood combustion of various fuels were determined with a system consisting of an aerosol particle mass analyzer and a scanning mobility particle sizer (APM-SMPS). A novel sampling chamber was combined to the system to enable measurements of highly fluctuating combustion processes. In addition, mass-mobility exponents (relates mass and mobility size) were determined from the density data to describe the shape of the particles. Particle size, type of fuel, combustion phase, and combustion conditions were found to have an effect on the effective density and the particle shape. For example, steady combustion phase produced agglomerates with effective density of roughly 1 g cm(-3) for small particles, decreasing to 0.25 g cm(-3) for 400 nm particles. The effective density was higher for particles emitted from glowing embers phase (ca. 1-2 g cm(-3)), and a clear size dependency was not observed as the particles were nearly spherical in shape. This study shows that a single value cannot be used for the effective density of particles emitted from wood combustion.

  14. Morphological operation based dense houses extraction from DSM

    NASA Astrophysics Data System (ADS)

    Li, Y.; Zhu, L.; Tachibana, K.; Shimamura, H.

    2014-08-01

    This paper presents a method of reshaping and extraction of markers and masks of the dense houses from the DSM based on mathematical morphology (MM). Houses in a digital surface model (DSM) are almost joined together in high-density housing areas, and most segmentation methods cannot completely separate them. We propose to label the markers of the buildings firstly and segment them into masks by watershed then. To avoid detecting more than one marker for a house or no marker at all due to its higher neighbour, the DSM is morphologically reshaped. It is carried out by a MM operation using the certain disk shape SE of the similar size to the houses. The sizes of the houses need to be estimated before reshaping. A granulometry generated by opening-by-reconstruction to the NDSM is proposed to detect the scales of the off-terrain objects. It is a histogram of the global volume of the top hats of the convex objects in the continuous scales. The obvious step change in the profile means that there are many objects of similar sizes occur at this scale. In reshaping procedure, the slices of the object are derived by morphological filtering at the detected continuous scales and reconstructed in pile as the dome. The markers are detected on the basis of the domes.

  15. Convergence and Divergence in the Evolution of Cat Skulls: Temporal and Spatial Patterns of Morphological Diversity

    PubMed Central

    Sakamoto, Manabu; Ruta, Marcello

    2012-01-01

    Background Studies of biological shape evolution are greatly enhanced when framed in a phylogenetic perspective. Inclusion of fossils amplifies the scope of macroevolutionary research, offers a deep-time perspective on tempo and mode of radiations, and elucidates life-trait changes. We explore the evolution of skull shape in felids (cats) through morphometric analyses of linear variables, phylogenetic comparative methods, and a new cladistic study of saber-toothed cats. Methodology/Principal Findings A new phylogenetic analysis supports the monophyly of saber-toothed cats (Machairodontinae) exclusive of Felinae and some basal felids, but does not support the monophyly of various saber-toothed tribes and genera. We quantified skull shape variation in 34 extant and 18 extinct species using size-adjusted linear variables. These distinguish taxonomic group membership with high accuracy. Patterns of morphospace occupation are consistent with previous analyses, for example, in showing a size gradient along the primary axis of shape variation and a separation between large and small-medium cats. By combining the new phylogeny with a molecular tree of extant Felinae, we built a chronophylomorphospace (a phylogeny superimposed onto a two-dimensional morphospace through time). The evolutionary history of cats was characterized by two major episodes of morphological divergence, one marking the separation between saber-toothed and modern cats, the other marking the split between large and small-medium cats. Conclusions/Significance Ancestors of large cats in the ‘Panthera’ lineage tend to occupy, at a much later stage, morphospace regions previously occupied by saber-toothed cats. The latter radiated out into new morphospace regions peripheral to those of extant large cats. The separation between large and small-medium cats was marked by considerable morphologically divergent trajectories early in feline evolution. A chronophylomorphospace has wider applications in reconstructing temporal transitions across two-dimensional trait spaces, can be used in ecophenotypical and functional diversity studies, and may reveal novel patterns of morphospace occupation. PMID:22792186

  16. Deep Generative Models of Galaxy Images for the Calibration of the Next Generation of Weak Lensing Surveys

    NASA Astrophysics Data System (ADS)

    Lanusse, Francois; Ravanbakhsh, Siamak; Mandelbaum, Rachel; Schneider, Jeff; Poczos, Barnabas

    2017-01-01

    Weak gravitational lensing has long been identified as one of the most powerful probes to investigate the nature of dark energy. As such, weak lensing is at the heart of the next generation of cosmological surveys such as LSST, Euclid or WFIRST.One particularly crititcal source of systematic errors in these surveys comes from the shape measurement algorithms tasked with estimating galaxy shapes. GREAT3, the last community challenge to assess the quality of state-of-the-art shape measurement algorithms has in particular demonstrated that all current methods are biased to various degrees and, more importantly, that these biases depend on the details of the galaxy morphologies. These biases can be measured and calibrated by generating mock observations where a known lensing signal has been introduced and comparing the resulting measurements to the ground-truth. Producing these mock observations however requires input galaxy images of higher resolution and S/N than the simulated survey, which typically implies acquiring extremely expensive space-based observations.The goal of this work is to train a deep generative model on already available Hubble Space Telescope data which can then be used to sample new galaxy images conditioned on parameters such as magnitude, size or redshift and exhibiting complex morphologies. Such model can allow us to inexpensively produce large set of realistic realistic images for calibration purposes.We implement a conditional generative model based on state-of-the-art deep learning methods and fit it to deep galaxy images from the COSMOS survey. The quality of the model is assessed by computing an extensive set of galaxy morphology statistics on the generated images. Beyond simple second moment statistics such as size and ellipticity, we apply more complex statistics specifically designed to be sensitive to disturbed galaxy morphologies. We find excellent agreement between the morphologies of real and model generated galaxies.Our results suggest that such deep generative models represent a reliable alternative to the acquisition of expensive high quality observations for generating the calibration data needed by the next generation of weak lensing surveys.

  17. The effect of size and composition on structural transitions in monometallic nanoparticles

    NASA Astrophysics Data System (ADS)

    Rossi, Kevin; Pavan, Luca; Soon, YeeYeen; Baletto, Francesca

    2018-02-01

    Predicting the morphological stability of nanoparticles is an essential step towards the accurate modelling of their chemophysical properties. Here we investigate solid-solid transitions in monometallic clusters of 0.5-2.0 nm diameter at finite temperatures and we report the complex dependence of the rearrangement mechanism on the nanoparticle's composition and size. The concerted Lipscomb's Diamond-Square-Diamond mechanisms which connects the decahedral or the cuboctahedral to the icosahedral basins, take place only below a material dependent critical size above which surface diffusion prevails and leads to low-symmetry and defected shapes still belonging to the initial basin.

  18. Morphological Variations in Conidia of Arthrobotrys oligospora on Different Media.

    PubMed

    Singh, R K; Kumar, Niranjan; Singh, K P

    2005-06-01

    Most commonly occurring predacious fungus Arthrobotrys oligospora showed great variation in size and shape of conidia on some media. The formation of larger conidia was recorded on beef extract and nutrient agar media. The length of conidia in Richard's YPSS, Sabouraud's, PDA and corn meal agar media was of medium size while smaller conidia were produced on Czapek's, Jensen's, Martin's medium. Maximum width of conidia was recorded on YPSS medium followed by Sabouraud's medium. The average size of spores on nematode infested corn meal agar medium was slightly increased than those on corn meal agar medium.

  19. A new approach to children's footwear based on foot type classification.

    PubMed

    Mauch, M; Grau, S; Krauss, I; Maiwald, C; Horstmann, T

    2009-08-01

    Current shoe designs do not allow for the comprehensive 3-D foot shape, which means they are unable to reproduce the wide variability in foot morphology. Therefore, the purpose of this study was to capture these variations of children's feet by classifying them into groups (types) and thereby provide a basis for their implementation in the design of children's shoes. The feet of 2867 German children were measured using a 3-D foot scanner. Cluster analysis was then applied to classify the feet into three different foot types. The characteristics of these foot types differ regarding their volume and forefoot shape both within and between shoe sizes. This new approach is in clear contrast to previous systems, since it captures the variability of foot morphology in a more comprehensive way by using a foot typing system and therefore paves the way for the unimpaired development of children's feet. Previous shoe systems do not allow for the wide variations in foot morphology. A new approach was developed regarding different morphological foot types based on 3-D measurements relevant in shoe construction. This can be directly applied to create specific designs for children's shoes.

  20. Vaccination and triploidy increase relative heart weight in farmed Atlantic salmon, Salmo salar L.

    PubMed

    Fraser, T W K; Mayer, I; Hansen, T; Poppe, T T; Skjaeraasen, J E; Koppang, E O; Fjelldal, P G

    2015-02-01

    Heart morphology is particularly plastic in teleosts and differs between farmed and wild Atlantic salmon. However, little is known about how different culture practices and sex affect heart morphology. This study investigated how vaccination, triploidy and sex affected heart size and heart morphology (ventricle shape, angle of the bulbus arteriosus) in farmed Atlantic salmon for 18 months following vaccination (from c. 50-3000 g body weight). In addition, hearts were examined histologically after 7 months in sea water. All fish sampled were sexually immature. Vaccinated fish had significantly heavier hearts relative to body weight and a more triangular ventricle than unvaccinated fish, suggesting a greater cardiac workload. Irrespective of time, triploids had significantly heavier hearts relative to body weight, a more acute angle of the bulbus arteriosus and less fat deposition in the epicardium than diploids. The ventricle was also more triangular in triploids than diploids at seawater transfer. Sex had transient effects on the angle of the bulbus arteriosus, but no effect on relative heart weight or ventricle shape. From a morphological perspective, the results indicate that vaccination and triploidy increase cardiac workload in farmed Atlantic salmon. © 2014 John Wiley & Sons Ltd.

  1. Sol-Gel processing of silica nanoparticles and their applications.

    PubMed

    Singh, Lok P; Bhattacharyya, Sriman K; Kumar, Rahul; Mishra, Geetika; Sharma, Usha; Singh, Garima; Ahalawat, Saurabh

    2014-11-06

    Recently, silica nanoparticles (SNPs) have drawn widespread attention due to their applications in many emerging areas because of their tailorable morphology. During the last decade, remarkable efforts have been made on the investigations for novel processing methodologies to prepare SNPs, resulting in better control of the size, shape, porosity and significant improvements in the physio-chemical properties. A number of techniques available for preparing SNPs namely, flame spray pyrolysis, chemical vapour deposition, micro-emulsion, ball milling, sol-gel etc. have resulted, a number of publications. Among these, preparation by sol-gel has been the focus of research as the synthesis is straightforward, scalable and controllable. Therefore, this review focuses on the recent progress in the field of synthesis of SNPs exhibiting ordered mesoporous structure, their distribution pattern, morphological attributes and applications. The mesoporous silica nanoparticles (MSNPs) with good dispersion, varying morphology, narrow size distribution and homogeneous porous structure have been successfully prepared using organic and inorganic templates. The soft template assisted synthesis using surfactants for obtaining desirable shapes, pores, morphology and mechanisms proposed has been reviewed. Apart from single template, double and mixed surfactants, electrolytes, polymers etc. as templates have also been intensively discussed. The influence of reaction conditions such as temperature, pH, concentration of reagents, drying techniques, solvents, precursor, aging time etc. have also been deliberated. These MSNPs are suitable for a variety of applications viz., in the drug delivery systems, high performance liquid chromatography (HPLC), biosensors, cosmetics as well as construction materials. The applications of these SNPs have also been briefly summarized. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. Diet, bite force and skull morphology in the generalist rodent morphotype.

    PubMed

    Maestri, R; Patterson, B D; Fornel, R; Monteiro, L R; de Freitas, T R O

    2016-11-01

    For many vertebrate species, bite force plays an important functional role. Ecological characteristics of a species' niche, such as diet, are often associated with bite force. Previous evidence suggests a biomechanical trade-off between rodents specialized for gnawing, which feed mainly on seeds, and those specialized for chewing, which feed mainly on green vegetation. We tested the hypothesis that gnawers are stronger biters than chewers. We estimated bite force and measured skull and mandible shape and size in 63 genera of a major rodent radiation (the myomorph sigmodontines). Analysis of the influence of diet on bite force and morphology was made in a comparative framework. We then used phylogenetic path analysis to uncover the most probable causal relationships linking diet and bite force. Both granivores (gnawers) and herbivores (chewers) have a similar high bite force, leading us to reject the initial hypothesis. Path analysis reveals that bite force is more likely influenced by diet than the reverse causality. The absence of a trade-off between herbivores and granivores may be associated with the generalist nature of the myomorph condition seen in sigmodontine rodents. Both gnawing and chewing sigmodontines exhibit similar, intermediate phenotypes, at least compared to extreme gnawers (squirrels) and chewers (chinchillas). Only insectivorous rodents appear to be moving towards a different direction in the shape space, through some notable changes in morphology. In terms of diet, natural selection alters bite force through changes in size and shape, indicating that organisms adjust their bite force in tandem with changes in food items. © 2016 European Society For Evolutionary Biology. Journal of Evolutionary Biology © 2016 European Society For Evolutionary Biology.

  3. Complex-Morphology Metal-Based Nanostructures: Fabrication, Characterization, and Applications

    PubMed Central

    Gentile, Antonella; Ruffino, Francesco; Grimaldi, Maria Grazia

    2016-01-01

    Due to their peculiar qualities, metal-based nanostructures have been extensively used in applications such as catalysis, electronics, photography, and information storage, among others. New applications for metals in areas such as photonics, sensing, imaging, and medicine are also being developed. Significantly, most of these applications require the use of metals in the form of nanostructures with specific controlled properties. The properties of nanoscale metals are determined by a set of physical parameters that include size, shape, composition, and structure. In recent years, many research fields have focused on the synthesis of nanoscale-sized metallic materials with complex shape and composition in order to optimize the optical and electrical response of devices containing metallic nanostructures. The present paper aims to overview the most recent results—in terms of fabrication methodologies, characterization of the physico-chemical properties and applications—of complex-morphology metal-based nanostructures. The paper strongly focuses on the correlation between the complex morphology and the structures’ properties, showing how the morphological complexity (and its nanoscale control) can often give access to a wide range of innovative properties exploitable for innovative functional device production. We begin with an overview of the basic concepts on the correlation between structural and optical parameters of nanoscale metallic materials with complex shape and composition, and the possible solutions offered by nanotechnology in a large range of applications (catalysis, electronics, photonics, sensing). The aim is to assess the state of the art, and then show the innovative contributions that can be proposed in this research field. We subsequently report on innovative, versatile and low-cost synthesis techniques, suitable for providing a good control on the size, surface density, composition and geometry of the metallic nanostructures. The main purpose of this study is the fabrication of functional nanoscale-sized materials, whose properties can be tailored (in a wide range) simply by controlling the structural characteristics. The modulation of the structural parameters is required to tune the plasmonic properties of the nanostructures for applications such as biosensors, opto-electronic or photovoltaic devices and surface-enhanced Raman scattering (SERS) substrates. The structural characterization of the obtained nanoscale materials is employed in order to define how the synthesis parameters affect the structural characteristics of the resulting metallic nanostructures. Then, macroscopic measurements are used to probe their electrical and optical properties. Phenomenological growth models are drafted to explain the processes involved in the growth and evolution of such composite systems. After the synthesis and characterization of the metallic nanostructures, we study the effects of the incorporation of the complex morphologies on the optical and electrical responses of each specific device. PMID:28335236

  4. Scaling and Accommodation of Jaw Adductor Muscles in Canidae

    PubMed Central

    Kemp, Graham J.; Jeffery, Nathan

    2016-01-01

    ABSTRACT The masticatory apparatus amongst closely related carnivoran species raises intriguing questions about the interplay between allometry, function, and phylogeny in defining interspecific variations of cranial morphology. Here we describe the gross structure of the jaw adductor muscles of several species of canid, and then examine how the muscles are scaled across the range of body sizes, phylogenies, and trophic groups. We also consider how the muscles are accommodated on the skull, and how this is influenced by differences of endocranial size. Data were collected for a suite of morphological metrics, including body mass, endocranial volume, and muscle masses and we used geometric morphometric shape analysis to reveal associated form changes. We find that all jaw adductor muscles scale isometrically against body mass, regardless of phylogeny or trophic group, but that endocranial volume scales with negative allometry against body mass. These findings suggest that head shape is partly influenced by the need to house isometrically scaling muscles on a neurocranium scaling with negative allometry. Principal component analysis suggests that skull shape changes, such as the relatively wide zygomatic arches and large sagittal crests seen in species with higher body masses, allow the skull to accommodate a relative enlargement of the jaw adductors compared with the endocranium. Anat Rec, 299:951–966, 2016. © 2016 The Authors The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology Published by Wiley Periodicals, Inc. PMID:27103346

  5. Low Dimensional Non-Crystallographic Metallic Nanostructures:. HRTEM Simulation, Models and Experimental Results

    NASA Astrophysics Data System (ADS)

    Rodríguez-López, J. L.; Montejano-Carrizales, J. M.; José-Yacamán, M.

    Modern nanoparticle research in the field of small metallic systems has confirmed that many nanoparticles take on some Platonic and Archimedean solids related shapes. A Platonic solid looks the same from any vertex, and intuitively they appear as good candidates for atomic equilibrium shapes. A very clear example is the icosahedral (Ih) particle that only shows {111} faces that contribute to produce a more rounded structure. Indeed, many studies report the Ih as the most stable particle at the size range r≤20 Å for noble gases and for some metals. In this review, we report on the structure and shape of mono- and bimetallic nanoparticles in the wide size range from 1-300 nm. First, we present AuPd nanoparticles in the 1-2 nm size range that show dodecahedral atomic growth packing, one of the Platonic solid shapes that have not been identified before in this small size range for metallic particles. Next, with particles in the size range of 2-5 nm, we present an energetic surface reconstruction phenomenon observed also on bimetallic nanoparticle systems of AuPd and AuCu, similar to a re-solidification effect observed during cooling process in lead clusters. These binary alloy nanoparticles show the fivefold edges truncated, resulting in {100} faces on decahedral structures, an effect largely envisioned and reported theoretically, with no experimental evidence in the literature before. Next nanostructure we review is a monometallic system in the size range of ≈5 nm that we termed the decmon. We present here some detailed geometrical analysis and experimental evidence that supports our models. Finally, in the size range of 100-300 nm, we present icosahedrally derived star gold nanocrystals which resembles the great stellated dodechaedron, which is a Kepler-Poisont solid. We conclude then that the shape or morphology of some mono- and bimetallic particles evolves with size following the sequence from atoms to the Platonic solids, and with a slightly greater particle's size, they tend to adopt Archimedean related shapes. If the particle's size is still greater, they tend to adopt shapes beyond the Archimedean (Kepler-Poisont) solids, reaching at the very end the bulk structure of solids. We demonstrate both experimentally and by means of computational simulations for each case that this structural atomic growth sequence is followed in such mono- and bimetallic nanoparticles.

  6. High surface area neodymium phosphate nano particles by modified aqueous sol-gel method

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sankar, Sasidharan; Warrier, Krishna Gopakumar, E-mail: wwarrierkgk@yahoo.co.in; Komban, Rajesh

    2011-12-15

    Graphical abstract: Synthesis of nano rod shaped neodymium phosphate particles with specific surface area as high as 107 m{sup 2} g{sup -1} and particles could be compacted and sintered at as low as 1300 Degree-Sign C to a density of 98.5% (theoretical) with an average grain size of {approx}1 {mu}m. Highlights: Black-Right-Pointing-Pointer Nano size neodymium phosphate is synthesized and characterized using a novel modified aqueous sol gel process. Black-Right-Pointing-Pointer Specific surface area above 100 m{sup 2} g{sup -1} achieved without the addition of any complexing agents. Black-Right-Pointing-Pointer High sintered density reported than the density obtained for powder synthesized through conventionalmore » solid state reaction. Black-Right-Pointing-Pointer The particles are nano sized and have rod shape morphology and are retained at higher temperatures. Black-Right-Pointing-Pointer An average grain size of {approx}1 {mu}m obtained for sintered NdPO{sub 4} after thermal etching at 1400 Degree-Sign C. -- Abstract: Synthesis of nano rod shaped neodymium phosphate (NdPO{sub 4}) particles with specific surface area as high as 107 m{sup 2}g{sup -1} and an average length of 50 nm with aspect ratio 5 was achieved using modified sol gel method. Crystallite size calculated from the X-ray diffraction data by applying Scherer equation was 5 nm for the precursor gel after calcination at 400 Degree-Sign C. NdPO{sub 4} was first precipitated from neodymium nitrate solution using phosphoric acid followed by peptization using dilute nitric acid and further gelation in ammonia atmosphere. The calcined gel powders were further characterized by surface area (Brunauer-Emmet-Teller nitrogen adsorption analysis), Transmission electron microscopy, scanning electron microscopy, UV-vis and FT-IR analysis. Transmission electron microscopy confirms the formation of rod like morphology from the sol, gel and the calcined particles in nano size range. These particles could be compacted and sintered at as low as 1300 Degree-Sign C to a density of 98.5% (theoretical) with an average grain size of {approx}1 {mu}m.« less

  7. Low-Pressure and Low-Temperature Hydriding-Pulverization-Dehydriding Method for Producing Shape Memory Alloy Powders

    NASA Astrophysics Data System (ADS)

    Murguia, Silvia Briseño; Clauser, Arielle; Dunn, Heather; Fisher, Wendy; Snir, Yoav; Brennan, Raymond E.; Young, Marcus L.

    2018-04-01

    Shape memory alloys (SMAs) are of high interest as active, adaptive "smart" materials for applications such as sensors and actuators due to their unique properties, including the shape memory effect and pseudoelasticity. Binary NiTi SMAs have shown the most desirable properties, and consequently have generated the most commercial success. A major challenge for SMAs, in particular, is their well-known compositional sensitivity. Therefore, it is critical to control the powder composition and morphology. In this study, a low-pressure, low-temperature hydriding-pulverization-dehydriding method for preparing well-controlled compositions, size, and size distributions of SMA powders from wires is presented. Starting with three different diameters of as-drawn martensitic NiTi SMA wires, pre-alloyed NiTi powders of various well-controlled sizes are produced by hydrogen charging the wires in a heated H3PO4 solution. After hydrogen charging for different charging times, the wires are pulverized and subsequently dehydrided. The wires and the resulting powders are characterized using scanning electron microscopy, differential scanning calorimetry, and X-ray diffraction. The relationship between the wire diameter and powder size is investigated as a function of hydrogen charging time. The rate of diameter reduction after hydrogen charging of wire is also examined. Finally, the recovery behavior due to the shape memory effect is investigated after dehydriding.

  8. Probing the Interplay between Dendritic Spine Morphology and Membrane-Bound Diffusion.

    PubMed

    Adrian, Max; Kusters, Remy; Storm, Cornelis; Hoogenraad, Casper C; Kapitein, Lukas C

    2017-11-21

    Dendritic spines are protrusions along neuronal dendrites that harbor the majority of excitatory postsynapses. Their distinct morphology, often featuring a bulbous head and small neck that connects to the dendritic shaft, has been shown to facilitate compartmentalization of electrical and cytoplasmic signaling stimuli elicited at the synapse. The extent to which spine morphology also forms a barrier for membrane-bound diffusion has remained unclear. Recent simulations suggested that especially the diameter of the spine neck plays a limiting role in this process. Here, we examine the connection between spine morphology and membrane-bound diffusion through a combination of photoconversion, live-cell superresolution experiments, and numerical simulations. Local photoconversion was used to obtain the timescale of diffusive equilibration in spines and followed by global sparse photoconversion to determine spine morphologies with nanoscopic resolution. These morphologies were subsequently used to assess the role of morphology on the diffusive equilibration. From the simulations, we could determine a robust relation between the equilibration timescale and a generalized shape factor calculated using both spine neck width and neck length, as well as spine head size. Experimentally, we found that diffusive equilibration was often slower, but rarely faster than predicted from the simulations, indicating that other biological confounders further reduce membrane-bound diffusion in these spines. This shape-dependent membrane-bound diffusion in mature spines may contribute to spine-specific compartmentalization of neurotransmitter receptors and signaling molecules and thereby support long-term plasticity of synaptic contacts. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  9. Development of antifouling surfaces to reduce bacterial attachment

    NASA Astrophysics Data System (ADS)

    Graham, Mary Viola

    Bacteria are exceptionally good at adhering to surfaces and forming complex structures known as biofilms. This process, known as biofouling, can cause problems for infrastructure (eg, clogging and damaging pipes), for the food industry (eg, contamination of processing surfaces and equipment, and for the medical industry (eg, contamination of indwelling medical devices). Accordingly, multiple strategies have been explored to combat biofouling, including chemical modification of surfaces, development of antibiotic coatings, and more recently, the use of engineered surface topography. When designed properly, engineered surface topographies can significantly reduce bacterial surface attachment, ultimately limiting surface colonization. In this work, we hypothesized that the morphology, size, spacing, and surface pre-treatment of topographical features should directly correlate with the size and shape of target organisms, in order to reduce biofouling. Topographical features with size and spacing from 0.25 to 2 mum were fabricated in silicone elastomer and tested against rod shaped bacteria with an average size of 0.5 x 2 mum and spherical bacteria (cocci) ranging from 0.5 - 1 μm in diameter. Antifouling properties of the different topographical features were tested in both static and flow-based assays, and under oxygen plasma-treated (hydrophilic) and untreated (hydrophobic) surface conditions. We found that surface pre-treatment universally affects the ability bacteria to attach to surfaces, while surface topography limits attachment in a manner dependent on the bacterial size/shape and the size/spacing of the topography.

  10. Morphology-Patterned Anisotropic Wetting Surface for Fluid Control and Gas-Liquid Separation in Microfluidics.

    PubMed

    Wang, Shuli; Yu, Nianzuo; Wang, Tieqiang; Ge, Peng; Ye, Shunsheng; Xue, Peihong; Liu, Wendong; Shen, Huaizhong; Zhang, Junhu; Yang, Bai

    2016-05-25

    This article shows morphology-patterned stripes as a new platform for directing flow guidance of the fluid in microfluidic devices. Anisotropic (even unidirectional) spreading behavior due to anisotropic wetting of the underlying surface is observed after integrating morphology-patterned stripes with a Y-shaped microchannel. The anisotropic wetting flow of the fluid is influenced by the applied pressure, dimensions of the patterns, including the period and depth of the structure, and size of the channels. Fluids with different surface tensions show different flowing anisotropy in our microdevice. Moreover, the morphology-patterned surfaces could be used as a microvalve, and gas-water separation in the microchannel was realized using the unidirectional flow of water. Therefore, benefiting from their good performance and simple fabrication process, morphology-patterned surfaces are good candidates to be applied in controlling the fluid behavior in microfluidics.

  11. Diversity among African Pygmies

    PubMed Central

    Ramírez Rozzi, Fernando V.; Sardi, Marina L.

    2010-01-01

    Although dissimilarities in cranial and post-cranial morphology among African pygmies groups have been recognized, comparative studies on skull morphology usually pull all pygmies together assuming that morphological characters are similar among them and different with respect to other populations. The main aim of this study is to compare cranial morphology between African pygmies and non-pygmies populations from Equatorial Africa derived from both the Eastern and the Western regions in order to test if the greatest morphological difference is obtained in the comparison between pygmies and non-pygmies. Thirty three-dimensional (3D) landmarks registered with Microscribe in four cranial samples (Western and Eastern pygmies and non-pygmies) were obtained. Multivariate analysis (generalized Procrustes analysis, Mahalanobis distances, multivariate regression) and complementary dimensions of size were evaluated with ANOVA and post hoc LSD. Results suggest that important cranial shape differentiation does occur between pygmies and non-pygmies but also between Eastern and Western populations and that size changes and allometries do not affect similarly Eastern and Western pygmies. Therefore, our findings raise serious doubt about the fact to consider African pygmies as a homogenous group in studies on skull morphology. Differences in cranial morphology among pygmies would suggest differentiation after divergence. Although not directly related to skull differentiation, the diversity among pygmies would probably suggest that the process responsible for reduced stature occurred after the split of the ancestors of modern Eastern and Western pygmies. PMID:21049030

  12. Diversity among African pygmies.

    PubMed

    Ramírez Rozzi, Fernando V; Sardi, Marina L

    2010-10-26

    Although dissimilarities in cranial and post-cranial morphology among African pygmies groups have been recognized, comparative studies on skull morphology usually pull all pygmies together assuming that morphological characters are similar among them and different with respect to other populations. The main aim of this study is to compare cranial morphology between African pygmies and non-pygmies populations from Equatorial Africa derived from both the Eastern and the Western regions in order to test if the greatest morphological difference is obtained in the comparison between pygmies and non-pygmies. Thirty three-dimensional (3D) landmarks registered with Microscribe in four cranial samples (Western and Eastern pygmies and non-pygmies) were obtained. Multivariate analysis (generalized Procrustes analysis, Mahalanobis distances, multivariate regression) and complementary dimensions of size were evaluated with ANOVA and post hoc LSD. Results suggest that important cranial shape differentiation does occur between pygmies and non-pygmies but also between Eastern and Western populations and that size changes and allometries do not affect similarly Eastern and Western pygmies. Therefore, our findings raise serious doubt about the fact to consider African pygmies as a homogenous group in studies on skull morphology. Differences in cranial morphology among pygmies would suggest differentiation after divergence. Although not directly related to skull differentiation, the diversity among pygmies would probably suggest that the process responsible for reduced stature occurred after the split of the ancestors of modern Eastern and Western pygmies.

  13. Morphological and Molecular Characterization of Paramecium (Viridoparamecium nov. subgen.) chlorelligerum Kahl, 1935 (Ciliophora)

    PubMed Central

    KREUTZ, MARTIN; STOECK, THORSTEN; FOISSNER, WILHELM

    2013-01-01

    We redescribe Paramecium chlorelligerum, a forgotten species, which Kahl (1935) briefly but precisely described in the addendum to his ciliate monographs as a Paramecium with symbiotic green algae. The redescription is based on classical morphological methods and the analysis of the small subunit (SSU) rDNA. Morphologically, P. chlorelligerum differs from P. (Chloroparamecium) bursaria, the second green species in the genus, by having a special swimming shape, the length of the caudal cilia, the size of the micronucleus, the size of the symbiotic algae, the contractile vacuoles (with collecting vesicles vs. collecting canals), and the number of excretory pores /contractile vacuole (1 vs. 2--3). The molecular investigations show that P. chlorelligerum forms a distinct branch distant from the P. (Chloroparamecium) bursaria clade. Thus, we classify P. chlorelligerum in a new subgenus: Paramecium (Viridoparamecium) chlorelligerum. The symbiotic alga belongs to the little-known genus Meyerella, as yet recorded only from the plankton of a North American lake. PMID:22827482

  14. Preparation of solid lipid nanoparticles as drug carriers for levothyroxine sodium with in vitro drug delivery kinetic characterization.

    PubMed

    Rostami, E; Kashanian, S; Azandaryani, A H

    2014-05-01

    The aim of this work was to produce and characterize solid lipid nanoparticles (SLN) containing levothyroxine sodium for oral administration, and to evaluate the kinetic release of these colloidal carriers. SLNs were prepared by microemulsion method. The particle size and zeta potential of levothyroxine sodium-loaded SLNs were determined to be around 153 nm,-43 mV (negatively charged), respectively by photon correlation spectroscopy. The levothyroxine entrapment efficiency was over 98%. Shape and surface morphology were determined by TEM and SEM. They revealed fairly spherical shape of nanoparticles.SLN formulation was stable over a period of 6 months. There were no significant changes in particle size, zeta potential and polydispersity index and entrapment efficiency, indicating that the developed SLNs were fairly stable.

  15. Ecological specialization and morphological diversification in Greater Antillean boas.

    PubMed

    Reynolds, R Graham; Collar, David C; Pasachnik, Stesha A; Niemiller, Matthew L; Puente-Rolón, Alberto R; Revell, Liam J

    2016-08-01

    Colonization of islands can dramatically influence the evolutionary trajectories of organisms, with both deterministic and stochastic processes driving adaptation and diversification. Some island colonists evolve extremely large or small body sizes, presumably in response to unique ecological circumstances present on islands. One example of this phenomenon, the Greater Antillean boas, includes both small (<90 cm) and large (4 m) species occurring on the Greater Antilles and Bahamas, with some islands supporting pairs or trios of body-size divergent species. These boas have been shown to comprise a monophyletic radiation arising from a Miocene dispersal event to the Greater Antilles, though it is not known whether co-occurrence of small and large species is a result of dispersal or in situ evolution. Here, we provide the first comprehensive species phylogeny for this clade combined with morphometric and ecological data to show that small body size evolved repeatedly on separate islands in association with specialization in substrate use. Our results further suggest that microhabitat specialization is linked to increased rates of head shape diversification among specialists. Our findings show that ecological specialization following island colonization promotes morphological diversity through deterministic body size evolution and cranial morphological diversification that is contingent on island- and species-specific factors. © 2016 The Author(s). Evolution © 2016 The Society for the Study of Evolution.

  16. Characteristics of Pebble- and Cobble-Sized Clasts Along the Curiosity Rover Traverse from Sol 0 to 800.

    NASA Astrophysics Data System (ADS)

    Aileen Yingst, R.; Cropper, Kevin; Blank, Jennifer; Goetz, Walter; Hamilton, Victoria; Hipkin, Victoria; Kah, Linda; Madsen, Morten Bo; Newsom, Horton; Williams, Rebecca; Bridges, John; Martinez-Frias, Jesús; King, Penelope

    2015-04-01

    The transport, sorting and abrasive processes that modify the loose surface fragments comprising a sedimentary population are best recorded in the morphologic characteristics of those fragments (also termed clasts or particles). Here we assess morphologic characteristics (size, shape, roundness, texture) of clasts in the pebble to cobble size range (2-256 mm) in Gale Crater imaged along the path of the Curisoty rover from sols 0 to 800. Pebble- to cobble-sized clasts along Curiosity's traverse most likely include geologic materials from the walls of fluvial canyons that debouch onto the crater floor, fragments shed from the central mound of the crater, and grains from modern eolian dunes. Our goal is to help constrain the boundaries of potential transport mechanisms important throughout the geologic history of the crater interior. Clast survey observations were taken on 162 sols. Most common clast types include: Type 1. These grey, fine-grained clasts are the most common type between sols 0 to ~650. Particles are angular to sub-angular (though sub-rounded clasts appear beginning ~sol 548), with flat facets terminating in sharp or slightly rounded edges. Surface texture is often smooth, but fresher facets can be rough and knobby at the sub-mm scale; occasionally, faint layers can be discerned. Wind-eroded features are common. Type 2. These clasts are gray and angular to sub-angular, displaying faces with circular or elongated concave-outward ellipsoids averaging 0.5-1.5 mm long-axis. A potential variant of this clast type is one in which ellipsoids are so deeply weathered that the particle takes on a scoria-like shape. Type 3. This class consists of angular to sub-angular void-rich clasts. These are rare, and disappear after ~sol 50, but reappear around sol 672. Type 4. These particles are angular to sub-angular and clast-rich. Clasts protrude as they wear, making surface textures jagged-looking. Type 5. Particles in this class vary in color, are equidimensional or somewhat elongate, and tend to be subrounded to well-rounded. Such fragments are a significant component of the clast population in some areas along traverse, often littering the surface surrounding larger conglomerate fragments. Type 6. These particles are angular to sub-rounded in shape, with a knobby, rugged surface texture. This type weathers to similar shapes as Type 4 but lacks protruding grains. Type 7. This class consists of platy or chunky fragments with a grainy surface texture. This type begins to appear around sol 746 and is the predominant clast type starting sol 780. This type is similar to the surrounding outcrop and thus likely did not travel far. The diverse morphology indicates that a complex interplay of varying lithologies, transport mechanisms, and environmental circumstances is responsible for the morphology observed. Morphology of clasts suggests that the majority of pebbles and cobbles have not been significantly altered in transport. The exceptions are pebbles that likely wore out of conglomerates, and a sub-rounded population near the base of Mt. Sharp that we are beginning to explore.

  17. Correlating hydrodynamic radii with that of two-dimensional nanoparticles

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Yue, Yuan; Kan, Yuwei; Clearfield, Abraham

    2015-12-21

    Dynamic light scattering (DLS) is one of the most adapted methods to measure the size of nanoparticles, as referred to the hydrodynamic radii (R{sub h}). However, the R{sub h} represents only that of three-dimensional spherical nanoparticles. In the present research, the size of two-dimensional (2D) nanoparticles of yttrium oxide (Y{sub 2}O{sub 3}) and zirconium phosphate (ZrP) was evaluated through comparing their hydrodynamic diameters via DLS with lateral sizes obtained using scanning and transmission electron microscopy. We demonstrate that the hydrodynamic radii are correlated with the lateral sizes of both square and circle shaped 2D nanoparticles. Two proportional coefficients, i.e., correctingmore » factors, are proposed for the Brownian motion status of 2D nanoparticles. The correction is possible by simplifying the calculation of integrals in the case of small thickness approximation. The correcting factor has great significance for investigating the translational diffusion behavior of 2D nanoparticles in a liquid and in effective and low-cost measurement in terms of size and morphology of shape-specific nanoparticles.« less

  18. Gonad morphology, oocyte development and spawning cycle of the calanoid copepod Acartia clausi

    NASA Astrophysics Data System (ADS)

    Eisfeld, Sonja M.; Niehoff, Barbara

    2007-09-01

    Information on gonad morphology and its relation to basic reproductive parameters such as clutch size and spawning frequency is lacking for Acartia clausi, a dominant calanoid copepod of the North Sea. To fill this gap, females of this species were sampled at Helgoland Roads from mid March to late May 2001. Gonad structure and oogenesis were studied using a combination of histology and whole-body-analysis. In addition, clutch size and spawning frequency were determined in incubation experiments, during which individual females were monitored at short intervals for 8 and 12 h, respectively. The histological analysis revealed that the ovary of A. clausi is w-shaped with two distinct tips pointing posteriorly. It is slightly different from that of other Acartia species and of other copepod taxa. From the ovary, two anterior diverticula extend into the head region, and two posterior diverticula extend to the genital opening in the abdomen. Developing oocytes change in shape and size, and in the appearance of the nucleus and the ooplasm. Based on these morphological characteristics, different oocyte development stages (OS) were identified. Mitotically dividing oogonia and young oocytes (OS 0) were restricted to the ovary, whereas vitellogenic oocytes (OS 1 4) were present in the diverticula. The development stage of the oocytes increased with distance to the ovary in both, anterior and posterior diverticula. Most advanced oocytes were situated ventrally, and their number varied between 1 and 18, at a median of 4. All oocyte development stages co-occur indicating that oogenesis in A. clausi is a continuous process. These morphological features reflect the reproductive traits of this species. In accordance with the low numbers of mature oocytes in the gonads, females usually produced small clutches of one to five eggs. Clutches were released throughout the entire observation period at intervals of 90 min (median) resulting in mean egg production rates of 18 28 eggs female-1 day-1.

  19. Body size and premolar evolution in the early-middle eocene euprimates of Wyoming.

    PubMed

    Jones, Katrina E; Rose, Kenneth D; Perry, Jonathan M G

    2014-01-01

    The earliest euprimates to arrive in North America were larger-bodied notharctids and smaller-bodied omomyids. Through the Eocene, notharctids generally continued to increase in body size, whereas omomyids generally radiated within small- and increasingly mid-sized niches in the middle Eocene. This study examines the influence of changing body size and diet on the evolution of the lower fourth premolar in Eocene euprimates. The P4 displays considerable morphological variability in these taxa. Despite the fact that most studies of primate dental morphology have focused on the molars, P4 can also provide important paleoecological insights. We analyzed the P4 from 177 euprimate specimens, representing 35 species (11 notharctids and 24 omomyids), in three time bins of approximately equal duration: early Wasatchian, late Wasatchian, and Bridgerian. Two-dimensional surface landmarks were collected from lingual photographs, capturing important variation in cusp position and tooth shape. Disparity metrics were calculated and compared for the three time bins. In the early Eocene, notharctids have a more molarized P4 than omomyids. During the Bridgerian, expanding body size range of omomyids was accompanied by a significant increase in P4 disparity and convergent evolution of the semimolariform condition in the largest omomyines. P4 morphology relates to diet in early euprimates, although patterns vary between families. Copyright © 2013 Wiley Periodicals, Inc.

  20. Cryptic lineage diversity, body size divergence, and sympatry in a species complex of Australian lizards (Gehyra).

    PubMed

    Moritz, Craig C; Pratt, Renae C; Bank, Sarah; Bourke, Gayleen; Bragg, Jason G; Doughty, Paul; Keogh, J Scott; Laver, Rebecca J; Potter, Sally; Teasdale, Luisa C; Tedeschi, Leonardo G; Oliver, Paul M

    2018-01-01

    Understanding the joint evolutionary and ecological underpinnings of sympatry among close relatives remains a key challenge in biology. This problem can be addressed through joint phylogenomic and phenotypic analysis of complexes of closely related lineages within, and across, species and hence representing the speciation continuum. For a complex of tropical geckos from northern Australia-Gehyra nana and close relatives-we combine mtDNA phylogeography, exon-capture sequencing, and morphological data to resolve independently evolving lineages and infer their divergence history and patterns of morphological evolution. Gehyra nana is found to include nine divergent lineages and is paraphyletic with four other species from the Kimberley region of north-west Australia. Across these 13 taxa, 12 of which are restricted to rocky habitats, several lineages overlap geographically, including on the diverse Kimberley islands. Morphological evolution is dominated by body size shifts, and both body size and shape have evolved gradually across the group. However, larger body size shifts are observed among overlapping taxa than among closely related parapatric lineages of G. nana, and sympatric lineages are more divergent than expected at random. Whether elevated body size differences among sympatric lineages are due to ecological sorting or character displacement remains to be determined. © 2017 The Author(s). Evolution © 2017 The Society for the Study of Evolution.

  1. Skyrmion morphology in ultrathin magnetic films

    NASA Astrophysics Data System (ADS)

    Gross, I.; Akhtar, W.; Hrabec, A.; Sampaio, J.; Martínez, L. J.; Chouaieb, S.; Shields, B. J.; Maletinsky, P.; Thiaville, A.; Rohart, S.; Jacques, V.

    2018-02-01

    Nitrogen-vacancy magnetic microscopy is employed in the quenching mode as a noninvasive, high-resolution tool to investigate the morphology of isolated skyrmions in ultrathin magnetic films. The skyrmion size and shape are found to be strongly affected by local pinning effects and magnetic field history. Micromagnetic simulations including a static disorder, based on the physical model of grain-to-grain thickness variations, reproduce all experimental observations and reveal the key role of disorder and magnetic history in the stabilization of skyrmions in ultrathin magnetic films. This work opens the way to an in-depth understanding of skyrmion dynamics in real, disordered media.

  2. Measuring the Symmetry of Supernova Remnants in the Radio

    NASA Astrophysics Data System (ADS)

    Stafford, Jennifer; Lopez, Laura A.

    2017-01-01

    Nearly 300 supernova remnants (SNRs) are known in the MIlky Way galaxy, and they offer an important means to study the explosions and interactions of supernovae at sub-pc scales. In this poster, we present analysis of the morphology of Galactic SNRs at radio wavelengths. Specifically, we measure the symmetry of several tens of SNRs in 6- and 20-cm Very Large Array images using a multipole expansion technique, the power-ratio method. We explore how the SNRs' morphology changes as a function of their size and estimated dynamical ages, with the aim of probing how SNR shapes evolve with time.

  3. Paclitaxel-loaded nanoparticles of star-shaped cholic acid-core PLA-TPGS copolymer for breast cancer treatment

    NASA Astrophysics Data System (ADS)

    Tang, Xiaolong; Cai, Shuyu; Zhang, Rongbo; Liu, Peng; Chen, Hongbo; Zheng, Yi; Sun, Leilei

    2013-10-01

    A system of novel nanoparticles of star-shaped cholic acid-core polylactide- d-α-tocopheryl polyethylene glycol 1000 succinate (CA-PLA-TPGS) block copolymer was developed for paclitaxel delivery for breast cancer treatment, which demonstrated superior in vitro and in vivo performance in comparison with paclitaxel-loaded poly( d, l-lactide- co-glycolide) (PLGA) nanoparticles and linear PLA-TPGS nanoparticles. The paclitaxel- or couramin 6-loaded nanoparticles were fabricated by a modified nanoprecipitation method and then characterized in terms of size, surface charge, surface morphology, drug encapsulation efficiency, and in vitro drug release. The CA-PLA-TPGS nanoparticles were found to be spherical in shape with an average size of around 120 nm. The nanoparticles were found to be stable, showing no change in the particle size and surface charge during 90-day storage of the aqueous solution. The release profiles of the paclitaxel-loaded nanoparticles exhibited typically biphasic release patterns. The results also showed that the CA-PLA-TPGS nanoparticles have higher antitumor efficacy than the PLA-TPGS nanoparticles and PLGA nanoparticles in vitro and in vivo. In conclusion, such nanoparticles of star-shaped cholic acid-core PLA-TPGS block copolymer could be considered as a potentially promising and effective strategy for breast cancer treatment.

  4. Why the Long Face? The Mechanics of Mandibular Symphysis Proportions in Crocodiles

    PubMed Central

    Walmsley, Christopher W.; Smits, Peter D.; Quayle, Michelle R.; McCurry, Matthew R.; Richards, Heather S.; Oldfield, Christopher C.; Wroe, Stephen; Clausen, Phillip D.; McHenry, Colin R.

    2013-01-01

    Background Crocodilians exhibit a spectrum of rostral shape from long snouted (longirostrine), through to short snouted (brevirostrine) morphologies. The proportional length of the mandibular symphysis correlates consistently with rostral shape, forming as much as 50% of the mandible’s length in longirostrine forms, but 10% in brevirostrine crocodilians. Here we analyse the structural consequences of an elongate mandibular symphysis in relation to feeding behaviours. Methods/Principal Findings Simple beam and high resolution Finite Element (FE) models of seven species of crocodile were analysed under loads simulating biting, shaking and twisting. Using beam theory, we statistically compared multiple hypotheses of which morphological variables should control the biomechanical response. Brevi- and mesorostrine morphologies were found to consistently outperform longirostrine types when subject to equivalent biting, shaking and twisting loads. The best predictors of performance for biting and twisting loads in FE models were overall length and symphyseal length respectively; for shaking loads symphyseal length and a multivariate measurement of shape (PC1– which is strongly but not exclusively correlated with symphyseal length) were equally good predictors. Linear measurements were better predictors than multivariate measurements of shape in biting and twisting loads. For both biting and shaking loads but not for twisting, simple beam models agree with best performance predictors in FE models. Conclusions/Significance Combining beam and FE modelling allows a priori hypotheses about the importance of morphological traits on biomechanics to be statistically tested. Short mandibular symphyses perform well under loads used for feeding upon large prey, but elongate symphyses incur high strains under equivalent loads, underlining the structural constraints to prey size in the longirostrine morphotype. The biomechanics of the crocodilian mandible are largely consistent with beam theory and can be predicted from simple morphological measurements, suggesting that crocodilians are a useful model for investigating the palaeobiomechanics of other aquatic tetrapods. PMID:23342027

  5. Electron Transfer Strategies Regulate Carbonate Mineral and Micropore Formation.

    PubMed

    Zeng, Zhirui; Tice, Michael M

    2018-01-01

    Some microbial carbonates are robust biosignatures due to their distinct morphologies and compositions. However, whether carbonates induced by microbial iron reduction have such features is unknown. Iron-reducing bacteria use various strategies to transfer electrons to iron oxide minerals (e.g., membrane-bound enzymes, soluble electron shuttles, nanowires, as well as different mechanisms for moving over or attaching to mineral surfaces). This diversity has the potential to create mineral biosignatures through manipulating the microenvironments in which carbonate precipitation occurs. We used Shewanella oneidensis MR-1, Geothrix fermentans, and Geobacter metallireducens GS-15, representing three different strategies, to reduce solid ferric hydroxide in order to evaluate their influence on carbonate and micropore formation (micro-size porosity in mineral rocks). Our results indicate that electron transfer strategies determined the morphology (rhombohedral, spherical, or long-chained) of precipitated calcium-rich siderite by controlling the level of carbonate saturation and the location of carbonate formation. Remarkably, electron transfer strategies also produced distinctive cell-shaped micropores in both carbonate and hydroxide minerals, thus producing suites of features that could potentially serve as biosignatures recording information about the sizes, shapes, and physiologies of iron-reducing organisms. Key Words: Microbial iron reduction-Micropore-Electron transfer strategies-Microbial carbonate. Astrobiology 18, 28-36.

  6. Nanosensors: towards morphological control of gas sensing activity. SnO2, In2O3, ZnO and WO3 case studies.

    PubMed

    Gurlo, Aleksander

    2011-01-01

    Anisotropy is a basic property of single crystals. Dissimilar facets/surfaces have different geometric and electronic structure that results in dissimilar functional properties. Several case studies unambiguously demonstrated that the gas sensing activity of metal oxides is determined by the nature of surfaces exposed to ambient gas. Accordingly, a control over crystal morphology, i.e. over the angular relationships, size and shape of faces in a crystal, is required for the development of better sensors with increased selectivity and sensitivity in the chemical determination of gases. The first step toward this nanomorphological control of the gas sensing properties is the design and synthesis of well-defined nanocrystals which are uniform in size, shape and surface structure. These materials possess the planes of the symmetrical set {hkl} and must therefore behave identically in chemical reactions and adsorption processes. Because of these characteristics, the form-controlled nanocrystals are ideal candidates for fundamental studies of mechanisms of gas sensing which should involve (i) gas sensing measurements on specific surfaces, (ii) their atomistic/quantum chemical modelling and (ii) spectroscopic information obtained on same surfaces under operation conditions of sensors.

  7. Ligand induced shape transformation of thorium dioxide nanocrystals.

    PubMed

    Wang, Gaoxue; Batista, Enrique R; Yang, Ping

    2018-04-27

    Nanocrystals (NCs) with size and shape dependent properties are a thriving research field. Remarkable progress has been made in the controlled synthesis of NCs of stable elements in the past two decades; however, the knowledge of the NCs of actinide compounds has been considerably limited due the difficulties in handling them both experimentally and theoretically. Actinide compounds, especially actinide oxides, play a critical role in many stages of the nuclear fuel cycle. Recently, a non-aqueous surfactant assisted approach has been developed for the synthesis of actinide oxide NCs with different morphologies, but an understanding of its control factors is still missing to date. Herein we present a comprehensive study on the low index surfaces of thorium dioxide (ThO2) and their interactions with relevant surfactant ligands using density functional calculations. A systematic picture on the thermodynamic stability of ThO2 NCs of different sizes and shapes is obtained employing empirical models based on the calculated surface energies. It is found that bare ThO2 NCs prefer the octahedral shape terminated by (111) surfaces. Oleic acid displays selective adsorption on the (110) surface, leading to the shape transformation from octahedrons to nanorods. Other ligands such as acetylacetone, oleylamine, and trioctylphosphine oxide do not modify the equilibrium shape of ThO2 NCs. This work provides atomic level insights into the anisotropic growth of ThO2 NCs that was recently observed in experiments, and thus may contribute to the controlled synthesis of actinide oxide NCs with well-defined size and shape for future applications.

  8. Stability and morphology of Ag nanoplatelets probed by depolarized dynamic light scattering

    NASA Astrophysics Data System (ADS)

    Zimbone, M.; Contino, A.; Maccarrone, G.; Musumeci, P.; Lo Faro, M. J.; Calcagno, L.

    2018-06-01

    The stability of silver nanoplatelet (NP) suspensions prepared with different concentrations of trisodium citrate (TSC) was studied by depolarized dynamic light scattering (DDLS) and UV–vis spectrometry. The morphology of the nanoparticles, as well as the color and stability of the sols, are tuned by the concentration of the capping agent. The nanoparticles prepared with high TSC concentration (>10‑4 M) are blue triangular NPs showing a slight truncation of the tips with aging. When low TSC concentrations are used, the color of the sols changes from blue to yellow with aging time and a strong modification of the morphology occurs: the nanoparticle shape changes from triangular to spherical. Remarkably, they show a high degree of anisotropy. The aging process was followed by the UV–vis spectra and by measuring the rotational diffusion coefficient by DDLS, providing information on the nanoparticle size and shape evolution. The high intensity of depolarized signal and the high value of rotational diffusion coefficient suggest that the aging process increases the thickness and the roughness of the nanoparticles

  9. Strain induced plasmon tuning in planar square-shaped aluminum nanoparticles array

    NASA Astrophysics Data System (ADS)

    Mokkath, Junais Habeeb

    2018-06-01

    Metal nanoparticle aggregate is an exciting platform for manipulating light-matter interactions at the nanoscale, thanks to the optically driven free electrons couple electrically across the inter-particle gap region. We use time dependent density functional theory calculations to investigate the optical response modulations in planar square-shaped aluminum nanoparticles array via morphology deformation (varying the inter-particle gap distance in the range of 2-20 Å) separately along one and two directions. We report the surprising observation that irrespective of the different morphology deformations, there exists a unique inter-particle gap distance of 12 Å for which, a maximum optical field enhancement can be achieved. We remark that plasmonic interaction between metal nanoparticles in an aggregate is controlled to a large extent by the size of the inter-particle gap distance. We believe that our quantum mechanical calculations will inspire and contribute to the design, control, and exploitation of aluminum based plasmonic devices.

  10. Does gene flow constrain adaptive divergence or vice versa? A test using ecomorphology and sexual isolation in Timema cristinae walking-sticks.

    PubMed

    Nosil, P; Crespi, B J

    2004-01-01

    Population differentiation often reflects a balance between divergent natural selection and the opportunity for homogenizing gene flow to erode the effects of selection. However, during ecological speciation, trait divergence results in reproductive isolation and becomes a cause, rather than a consequence, of reductions in gene flow. To assess both the causes and the reproductive consequences of morphological differentiation, we examined morphological divergence and sexual isolation among 17 populations of Timema cristinae walking-sticks. Individuals from populations adapted to using Adenostoma as a host plant tended to exhibit smaller overall body size, wide heads, and short legs relative to individuals using Ceonothus as a host. However, there was also significant variation in morphology among populations within host-plant species. Mean trait values for each single population could be reliably predicted based upon host-plant used and the potential for homogenizing gene flow, inferred from the size of the neighboring population using the alternate host and mitochondrial DNA estimates of gene flow. Morphology did not influence the probability of copulation in between-population mating trials. Thus, morphological divergence is facilitated by reductions in gene flow, but does not cause reductions in gene flow via the evolution of sexual isolation. Combined with rearing data indicating that size and shape have a partial genetic basis, evidence for parallel origins of the host-associated forms, and inferences from functional morphology, these results indicate that morphological divergence in T. cristinae reflects a balance between the effects of host-specific natural selection and gene flow. Our findings illustrate how data on mating preferences can help determine the causal associations between trait divergence and levels of gene flow.

  11. A morphological and life history comparison between desert populations of a sit-and-pursue antlion, in reference to a co-occurring pit-building antlion.

    PubMed

    Scharf, Inon; Filin, Ido; Subach, Aziz; Ovadia, Ofer

    2009-10-01

    Although most antlion species do not construct pits, the vast majority of studies on antlions focused on pit-building species. We report here on a transplant experiment aiming to test for morphological and life history differences between two desert populations of a sit-and-pursue antlion species, Lopezus fedtschenkoi (Neuroptera: Myrmeleontidae), originating from habitats, which mainly differ in plant cover and productivity. We raised the antlion larvae in environmental chambers simulating either hyper-arid or Mediterranean climate. We found significant differences in the morphology and life history of L. fedtschenkoi larvae between the two populations. For example, the larvae originating from the more productive habitat pupated faster and had a higher growth rate. In agreement with the temperature-size rule, antlions reached higher final mass in the colder Mediterranean climate and exhibited a higher growth rate, but there was no difference in their developmental time. Observed differences in morphology between populations as well as those triggered by climate growing conditions could be explained by differences in size allometry. We also provide a quantitative description of the allometric growth axis, based on 12 morphological traits. Comparing the responses of L. fedtschenkoi with those observed in a co-occurring pit-building antlion indicated that there were neither shape differences that are independent of size nor was there a difference in the plasticity level between the two species.

  12. Facile and green synthesis of mesoporous Co3O4 nanocubes and their applications for supercapacitors.

    PubMed

    Liu, Xiangmei; Long, Qing; Jiang, Chunhui; Zhan, Beibei; Li, Chen; Liu, Shujuan; Zhao, Qiang; Huang, Wei; Dong, Xiaochen

    2013-07-21

    Nanostructured Co3O4 materials attracted significant attention due to their exceptional electrochemical (pseudo-capacitive) properties. However, rigorous preparation conditions are needed to control the size (especially nanosize), morphology and size distribution of the products obtained by conventional methods. Herein, we describe a novel one step shape-controlled synthesis of uniform Co3O4 nanocubes with a size of 50 nm with the existence of mesoporous carbon nanorods (meso-CNRs). In this synthesis process, meso-CNRs not only act as a heat receiver to directly obtain Co3O4 eliminating the high-temperature post-calcination, but also control the morphology of the resulting Co3O4 to form nanocubes with uniform distribution. More strikingly, mesoporous Co3O4 nanocubes are obtained by further thermal treatment. The structure and morphology of the samples were characterized by scanning electron microscopy, transmission electron microscopy and X-ray diffraction. A possible formation mechanism of mesoporous Co3O4 nanocubes is proposed here. Electrochemical tests have revealed that the prepared mesoporous Co3O4 nanocubes demonstrate a remarkable performance in supercapacitor applications due to the porous structure, which endows fast ion and electron transfer.

  13. Relationships between oral morphology and feeding style in the Ungulata: a phylogenetically controlled evaluation.

    PubMed

    Pérez-Barbería, F J; Gordon, I J

    2001-05-22

    In ungulates it is argued that specialization in the consumption of a particular type of food (feeding style) is reflected in morphological adaptations of the organs involved in the selection, processing and digestion of food. We analysed the differences in size and morphology of some oral traits that have been functionally related to food-selection ability (muzzle width, incisor-arcade shape, incisor shape), prehension of food (incisor protrusion), food comminution (molar occlusal surface area, hypsodonty (high-crowned molars)) and intake rate (incisor breadth) between ungulate species with different feeding styles (browser, mixed feeder, grazer). Grazers were characterized by large-body-size species. After controlling only for body mass, we found that grazers had wider muzzles and incisors, more-protruding incisors and more-bulky and higher-crowned molars than did mixed feeders and browsers. When the analyses took into account both body mass and phylogeny, only body mass and two out of the three hypsodonty indexes used remained significantly different between feeding styles. Browsers were smaller, on average, than mixed feeders and grazers, whilst grazers and mixed feeders did not differ in size. Also, browsers had shorter and less-bulky molars than did mixed feeders and grazers; the latter two feeding styles did not differ from each other in any of the hypsodonty indexes. We conclude that the adaptation to different dietary types in most of the oral traits studied is subsumed by the effects of body mass and the sharing of common ancestors. We hypothesize that differences in the ability to exploit different food resources primarily result from differences in body mass between species, and also discuss why hypsodonty characterizes feeding styles.

  14. Relationships between oral morphology and feeding style in the Ungulata: a phylogenetically controlled evaluation.

    PubMed Central

    Pérez-Barbería, F. J.; Gordon, I. J.

    2001-01-01

    In ungulates it is argued that specialization in the consumption of a particular type of food (feeding style) is reflected in morphological adaptations of the organs involved in the selection, processing and digestion of food. We analysed the differences in size and morphology of some oral traits that have been functionally related to food-selection ability (muzzle width, incisor-arcade shape, incisor shape), prehension of food (incisor protrusion), food comminution (molar occlusal surface area, hypsodonty (high-crowned molars)) and intake rate (incisor breadth) between ungulate species with different feeding styles (browser, mixed feeder, grazer). Grazers were characterized by large-body-size species. After controlling only for body mass, we found that grazers had wider muzzles and incisors, more-protruding incisors and more-bulky and higher-crowned molars than did mixed feeders and browsers. When the analyses took into account both body mass and phylogeny, only body mass and two out of the three hypsodonty indexes used remained significantly different between feeding styles. Browsers were smaller, on average, than mixed feeders and grazers, whilst grazers and mixed feeders did not differ in size. Also, browsers had shorter and less-bulky molars than did mixed feeders and grazers; the latter two feeding styles did not differ from each other in any of the hypsodonty indexes. We conclude that the adaptation to different dietary types in most of the oral traits studied is subsumed by the effects of body mass and the sharing of common ancestors. We hypothesize that differences in the ability to exploit different food resources primarily result from differences in body mass between species, and also discuss why hypsodonty characterizes feeding styles. PMID:11375086

  15. Lilium spp. pollen in China (Liliaceae): Taxonomic and Phylogenetic Implications and Pollen Evolution Related to Environmental Conditions

    PubMed Central

    Du, Yun-peng; Wei, Chi; Wang, Zhong-xuan; Li, Shuang; He, Heng-bin; Jia, Gui-xia

    2014-01-01

    Recent molecular and karyologic studies have significantly modified delimitation of Lilium. However, despite the importance of pollen evolution in the genus comprehensive studies with electron microscopy and evaluation of pollen evolution are lacking. Therefore, we studied pollen morphology in a sample of 65 individuals from 37 taxa covering all the sections distributed in the world, using scanning electron microscopy. Our collection of 49 individuals from 21 taxa covering all five sections in China was also included in the database. We found pollen tetrads in L. bakerianum. Based on present and previous studies, our results suggest that pollen from L. formosanum should be classified as a new type, Formosanum. Combined with morphological and molecular evidence, pollen sculpture patterns appear to reflect phylogenetic relationships and are useful for species or subsection delimitation. Based on a comprehensive survey and correlation with potential functional implications, we propose the following hypothesis: evolution of an exine sculpture shows pollen type trends from Martagon → Callose → Concolor → Formosanum. The evolutionary trend regarding pollen sculpture and size could be related to selective pressure to adapt to environmental conditions. Pollen size and shape showed a significantly positive correlation with annual precipitation, and smaller pollen grains appear to adapt better in habitats with extreme conditions. Evolution trends in exine sculpture do not appear to be definitively correlated with pollen size and shape. PMID:24498208

  16. Lilium spp. pollen in China (Liliaceae): taxonomic and phylogenetic implications and pollen evolution related to environmental conditions.

    PubMed

    Du, Yun-peng; Wei, Chi; Wang, Zhong-xuan; Li, Shuang; He, Heng-bin; Jia, Gui-xia

    2014-01-01

    Recent molecular and karyologic studies have significantly modified delimitation of Lilium. However, despite the importance of pollen evolution in the genus comprehensive studies with electron microscopy and evaluation of pollen evolution are lacking. Therefore, we studied pollen morphology in a sample of 65 individuals from 37 taxa covering all the sections distributed in the world, using scanning electron microscopy. Our collection of 49 individuals from 21 taxa covering all five sections in China was also included in the database. We found pollen tetrads in L. bakerianum. Based on present and previous studies, our results suggest that pollen from L. formosanum should be classified as a new type, Formosanum. Combined with morphological and molecular evidence, pollen sculpture patterns appear to reflect phylogenetic relationships and are useful for species or subsection delimitation. Based on a comprehensive survey and correlation with potential functional implications, we propose the following hypothesis: evolution of an exine sculpture shows pollen type trends from Martagon → Callose → Concolor → Formosanum. The evolutionary trend regarding pollen sculpture and size could be related to selective pressure to adapt to environmental conditions. Pollen size and shape showed a significantly positive correlation with annual precipitation, and smaller pollen grains appear to adapt better in habitats with extreme conditions. Evolution trends in exine sculpture do not appear to be definitively correlated with pollen size and shape.

  17. Morphoregulation of teeth: modulating the number, size, shape and differentiation by tuning Bmp activity

    PubMed Central

    Plikus, Maksim V.; Zeichner-David, Maggie; Mayer, Julie-Ann; Reyna, Julia; Bringas, Pablo; Thewissen, J. G. M.; Snead, Malcolm L.; Chai, Yang; Chuong, Cheng-Ming

    2015-01-01

    SUMMARY During development and evolution, the morphology of ectodermal organs can be modulated so that an organism can adapt to different environments. We have proposed that morphoregulation can be achieved by simply tilting the balance of molecular activity. We test the principles by analyzing the effects of partial downregulation of Bmp signaling in oral and dental epithelia of the keratin 14-Noggin transgenic mouse. We observed a wide spectrum of tooth phenotypes. The dental formula changed from 1.0.0.3/1.0.0.3 to 1.0.0.2(1)/1.0.0.0. All mandibular and M3 maxillary molars were selectively lost because of the developmental block at the early bud stage. First and second maxillary molars were reduced in size, exhibited altered crown patterns, and failed to form multiple roots. In these mice, incisors were not transformed into molars. Histogenesis and differentiation of ameloblasts and odontoblasts in molars and incisors were abnormal. Lack of enamel caused misocclusion of incisors, leading to deformation and enlargement in size. Therefore, subtle differences in the level, distribution, and timing of signaling molecules can have major morphoregulatory consequences. Modulation of Bmp signaling exemplifies morphoregulation hypothesis: simple alteration of key signaling pathways can be used to transform a prototypical conical-shaped tooth into one with complex morphology. The involvement of related pathways and the implication of morphoregulation in tooth evolution are discussed. PMID:16174037

  18. METABOLIC AND MORPHOLOGICAL OBSERVATIONS ON THE EFFECT OF SURFACE-ACTIVE AGENTS ON LEUKOCYTES

    PubMed Central

    Graham, R. C.; Karnovsky, M. J.; Shafer, A. W.; Glass, E. A.; Karnovsky, Manfred L.

    1967-01-01

    Morphological and metabolic observations have been made on the effects of endotoxin, deoxycholate, and digitonin (at less than 50 µg/ml) on polymorphonuclear leukocytes and mononuclear cells. The agents stimulate the respiration and glucose oxidation of these cells in a manner similar to that seen during phagocytosis. Electron microscopy revealed no morphological changes with the first two agents, but dramatic membrane changes were seen in the case of digitonin. Here tubular projections of characteristic size and shape formed on and split off the membrane. All the agents stimulated uptake of inulin, but efforts to demonstrate increased pinocytosis by electron microscopy have not so far succeeded, probably due to limitations in present experimental techniques. PMID:6034482

  19. Effects of environmental disturbance on phenotypic variation: an integrated assessment of canalization, developmental stability, modularity, and allometry in lizard head shape.

    PubMed

    Lazić, Marko M; Carretero, Miguel A; Crnobrnja-Isailović, Jelka; Kaliontzopoulou, Antigoni

    2015-01-01

    When populations experience suboptimal conditions, the mechanisms involved in the regulation of phenotypic variation can be challenged, resulting in increased phenotypic variance. This kind of disturbance can be diagnosed by using morphometric tools to study morphological patterns at different hierarchical levels and evaluate canalization, developmental stability, integration, modularity, and allometry. We assess the effect of urbanization on phenotypic variation in the common wall lizard (Podarcis muralis) by using geometric morphometrics to assess disturbance to head shape development. The head shapes of urban lizards were more variable and less symmetric, suggesting that urban living is more likely to disturb development. Head shape variation was congruent within and across individuals, which indicated that canalization and developmental stability are two related phenomena in these organisms. Furthermore, urban lizards exhibited smaller mean head sizes, divergent size-shape allometries, and increased deviation from within-group allometric lines. This suggests that mechanisms regulating head shape allometry may also be disrupted. The integrated evaluation of several measures of developmental instability at different hierarchical levels, which provided in this case congruent results, can be a powerful methodological guide for future studies, as it enhances the detection of environmental disturbances on phenotypic variation and aids biological interpretation of the results.

  20. The role of neurocranial shape in defining the boundaries of an expanded Homo erectus hypodigm.

    PubMed

    Baab, Karen L

    2016-03-01

    The main goals of this study were to evaluate the distinctiveness of Homo erectus neurocranial shape relative to other closely related species, and assess the likelihood that particular fossils were correctly attributed to H. erectus given how shape variation related to geography, time and brain size. This was accomplished through analyses of several sets of landmarks designed to maximize the fossil sample, including 24 putative H. erectus fossils. The question of taxonomic differentiation was initially assessed for the type specimen (Trinil II) and morphologically similar Sangiran fossils and subsequently for increasingly inclusive definitions of H. erectus. Results indicated that H. erectus fossils from China, Indonesia, Georgia and East Africa shared a neurocranial shape that was distinct from that of other Plio-Pleistocene Homo taxa, a pattern only partially accounted for by brain size. Early Indonesian H. erectus formed a morphological "bridge" between earlier and later populations assigned to H. erectus from Africa and Asia, respectively. These results were combined with discrete characters to create a more complete species definition for H. erectus. There were two notable exceptions to the general pattern of H. erectus uniqueness. The 0.8-1.0 Ma (millions of years ago) Daka calvaria from Ethiopia consistently grouped with mid-Pleistocene Homo, including Bodo and Kabwe, rather than African or Asian H. erectus. In addition, Daka also exhibited several traits derived for mid-Pleistocene Homo, and its scaling pattern mirrored mid-Pleistocene Homo rather than H. erectus. Daka may have belonged to an "advanced" H. erectus population close to the root of Homo heidelbergensis sensu lato (s.l.), or to an early population of H. heidelbergensis s.l.. The 1.5 Ma KNM-ER 42700 specimen from Kenya exhibited a unique calvarial shape distinct from H. erectus despite the exclusion of problematic landmarks from the frontal bone. These unique aspects of shape were not present in two other subadult fossils, KNM-WT 15000 and D2700. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Effect of reinforcement morphology on matrix microcracking

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sridhar, N.; Srolovitz, D.J.; Rickman, J.M.

    1996-03-01

    The authors quantitatively examine the conditions under which a particle matrix misfit leads to matrix crack growth as a function of inclusion shape. Such misfit stresses and cracks can be generated by thermal expansion mismatch, generated by cooling a brittle matrix containing ductile inclusions. Using fracture mechanics and perturbation theory, they analyze the case of a penny-shaped crack interacting with a misfitting spheroidal inclusion. A simple and direct relationship is established between the strain energy release rate and the physical and geometrical properties of the system including: the thermal expansion mismatch, temperature change, the crack and inclusion sizes, the elasticmore » properties of the medium and the shape of the inclusion. In particular, the effects of inclusion shape on the stress intensity factors and strain energy release rate are analytically determined for nearly spherical inclusions. The authors use this information to determine the minimum crack size for crack growth to occur and the maximum size to which cracks may grown. The maximum crack size corresponds to the case where the elastic strain energy released upon crack growth is no longer sufficient to compensate for energy expended in extending the crack as the crack is growing into the rapidly decreasing stress field. The authors employ a nominally exact numerical procedure to study the effects of whiskers and platelets (i.e. spheroids very different from spheres) on matrix cracking. It is found that upon cooling a composite containing ductile inclusions, the propensity for matrix cracking is maximized for reinforcement shapes close to that of a sphere.« less

  2. Wrinkling Non-Spherical Particles and Its Application in Cell Attachment Promotion

    NASA Astrophysics Data System (ADS)

    Li, Minggan; Joung, Dehi; Hughes, Bethany; Waldman, Stephen D.; Kozinski, Janusz A.; Hwang, Dae Kun

    2016-07-01

    Surface wrinkled particles are ubiquitous in nature and present in different sizes and shapes, such as plant pollens and peppercorn seeds. These natural wrinkles provide the particles with advanced functions to survive and thrive in nature. In this work, by combining flow lithography and plasma treatment, we have developed a simple method that can rapidly create wrinkled non-spherical particles, mimicking the surface textures in nature. Due to the oxygen inhibition in flow lithography, the non-spherical particles synthesized in a microfluidic channel are covered by a partially cured polymer (PCP) layer. When exposed to plasma treatment, this PCP layer rapidly buckles, forming surface-wrinkled particles. We designed and fabricated various particles with desired shapes and sizes. The surfaces of these shapes were tuned to created wrinkle morphologies by controlling UV exposure time and the washing process. We further demonstrated that wrinkles on the particles significantly promoted cell attachment without any chemical modification, potentially providing a new route for cell attachment for various biomedical applications.

  3. Mechanochemical synthesis of fluorescent carbon dots from cellulose powders

    NASA Astrophysics Data System (ADS)

    Chae, Ari; Ram Choi, Bo; Choi, Yujin; Jo, Seongho; Kang, Eun Bi; Lee, Hyukjin; Park, Sung Young; In, Insik

    2018-04-01

    A novel mechanochemical method was firstly developed to synthesize carbon nanodots (CNDs) or carbon nano-onions (CNOs) through high-pressure homogenization of cellulose powders as naturally abundant resource depending on the treatment times. While CNDs (less than 5 nm in size) showed spherical and amorphous morphology, CNOs (10-50 nm in size) presented polyhedral shape, and onion-like outer lattice structure, graphene-like interlattice spacing of 0.36 nm. CNOs showed blue emissions, moderate dispersibility in aqueous media, and high cell viability, which enables efficient fluorescence imaging of cellular media.

  4. Automatic archaeological feature extraction from satellite VHR images

    NASA Astrophysics Data System (ADS)

    Jahjah, Munzer; Ulivieri, Carlo

    2010-05-01

    Archaeological applications need a methodological approach on a variable scale able to satisfy the intra-site (excavation) and the inter-site (survey, environmental research). The increased availability of high resolution and micro-scale data has substantially favoured archaeological applications and the consequent use of GIS platforms for reconstruction of archaeological landscapes based on remotely sensed data. Feature extraction of multispectral remotely sensing image is an important task before any further processing. High resolution remote sensing data, especially panchromatic, is an important input for the analysis of various types of image characteristics; it plays an important role in the visual systems for recognition and interpretation of given data. The methods proposed rely on an object-oriented approach based on a theory for the analysis of spatial structures called mathematical morphology. The term "morphology" stems from the fact that it aims at analysing object shapes and forms. It is mathematical in the sense that the analysis is based on the set theory, integral geometry, and lattice algebra. Mathematical morphology has proven to be a powerful image analysis technique; two-dimensional grey tone images are seen as three-dimensional sets by associating each image pixel with an elevation proportional to its intensity level. An object of known shape and size, called the structuring element, is then used to investigate the morphology of the input set. This is achieved by positioning the origin of the structuring element to every possible position of the space and testing, for each position, whether the structuring element either is included or has a nonempty intersection with the studied set. The shape and size of the structuring element must be selected according to the morphology of the searched image structures. Other two feature extraction techniques were used, eCognition and ENVI module SW, in order to compare the results. These techniques were applied to different archaeological sites in Turkmenistan (Nisa) and in Iraq (Babylon); a further change detection analysis was applied to the Babylon site using two HR images as a pre-post second gulf war. We had different results or outputs, taking into consideration the fact that the operative scale of sensed data determines the final result of the elaboration and the output of the information quality, because each of them was sensitive to specific shapes in each input image, we had mapped linear and nonlinear objects, updating archaeological cartography, automatic change detection analysis for the Babylon site. The discussion of these techniques has the objective to provide the archaeological team with new instruments for the orientation and the planning of a remote sensing application.

  5. The role of habitat shift in the evolution of lizard morphology: evidence from tropical Tropidurus

    PubMed Central

    Vitt, Laurie J.; Caldwell, Janalee P.; Zani, Peter A.; Titus, Tom A.

    1997-01-01

    We compared morphology of two geographically close populations of the tropical lizard Tropidurus hispidus to test the hypothesis that habitat structure influences the evolution of morphology and ecology at the population level. T. hispidus isolated on a rock outcrop surrounded by tropical forest use rock crevices for refuge and appear dorsoventrally compressed compared with those in open savanna. A principal components analysis revealed that the populations were differentially distributed along an axis representing primarily three components of shape: body width, body height, and hind-leg length. Morphological divergence was supported by a principal components analysis of size-free morphological variables. Mitochondrial DNA sequences of ATPase 6 indicate that these populations are closely related relative to other T. hispidus, the rock outcrop morphology and ecology are derived within T. hispidus, and morphological and ecological divergence has occurred more rapidly than genetic divergence. This suggests that natural selection can rapidly adjust morphology and ecology in response to a recent history of exposure to habitats differing in structure, a result heretofore implied from comparative studies among lizard species. PMID:9108063

  6. The role of habitat shift in the evolution of lizard morphology: evidence from tropical Tropidurus.

    PubMed

    Vitt, L J; Caldwell, J P; Zani, P A; Titus, T A

    1997-04-15

    We compared morphology of two geographically close populations of the tropical lizard Tropidurus hispidus to test the hypothesis that habitat structure influences the evolution of morphology and ecology at the population level. T. hispidus isolated on a rock outcrop surrounded by tropical forest use rock crevices for refuge and appear dorsoventrally compressed compared with those in open savanna. A principal components analysis revealed that the populations were differentially distributed along an axis representing primarily three components of shape: body width, body height, and hind-leg length. Morphological divergence was supported by a principal components analysis of size-free morphological variables. Mitochondrial DNA sequences of ATPase 6 indicate that these populations are closely related relative to other T. hispidus, the rock outcrop morphology and ecology are derived within T. hispidus, and morphological and ecological divergence has occurred more rapidly than genetic divergence. This suggests that natural selection can rapidly adjust morphology and ecology in response to a recent history of exposure to habitats differing in structure, a result heretofore implied from comparative studies among lizard species.

  7. Integration of motor proteins - towards an ATP fueled soft actuator.

    PubMed

    Kakugo, Akira; Shikinaka, Kazuhiro; Gong, Jian Ping

    2008-09-01

    We present a soft bio-machine constructed from biological motors (actin/myosin). We have found that chemically cross-linked polymer-actin complex gel filaments can move on myosin coated surfaces with a velocity as high as that of native F-actin, by coupling to ATP hydrolysis. Additionally, it is shown that the velocity of polymer-actin complex gel depends on the species of polycations binding to the F-actins. Since the design of functional actuators of well-defined size and morphology is important, the structural behavior of polymer-actin complexes has been investigated. Our results show that the morphology and growth size of polymer-actin complex can be controlled by changes in the electrostatic interactions between F-actins and polycations. Our results indicate that bio actuators with desired shapes can be created by using a polymer-actin complex.

  8. Self-assembly of self-limiting monodisperse supraparticles from polydisperse nanoparticles

    NASA Astrophysics Data System (ADS)

    Xia, Yunsheng; Nguyen, Trung Dac; Yang, Ming; Lee, Byeongdu; Santos, Aaron; Podsiadlo, Paul; Tang, Zhiyong; Glotzer, Sharon C.; Kotov, Nicholas A.

    2011-09-01

    Nanoparticles are known to self-assemble into larger structures through growth processes that typically occur continuously and depend on the uniformity of the individual nanoparticles. Here, we show that inorganic nanoparticles with non-uniform size distributions can spontaneously assemble into uniformly sized supraparticles with core-shell morphologies. This self-limiting growth process is governed by a balance between electrostatic repulsion and van der Waals attraction, which is aided by the broad polydispersity of the nanoparticles. The generic nature of the interactions creates flexibility in the composition, size and shape of the constituent nanoparticles, and leads to a large family of self-assembled structures, including hierarchically organized colloidal crystals.

  9. Physical characterization of fine particulate matter inside the public transit buses fueled by biodiesel in Toledo, Ohio.

    PubMed

    Shandilya, Kaushik K; Kumar, Ashok

    2011-06-15

    This study presents the physical characteristics of fine particulate matter (PM) collected inside the urban-public transit buses in Toledo, OH. These buses run on 20% biodiesel blended with ultra-low sulfur diesel (ULSD) (B20). For risk analysis, it is crucial to know the modality of the size distribution and the shape factor of PM collected inside the bus. The number-size distribution, microstructure, and aspect ratio of fine PM filter samples collected in the urban-public transit buses were measured for three years (2007-2009), using an environmental scanning electron microscope (ESEM) coupled with energy dispersive X-ray spectrometry (EDX). Only the reproducible results from repeated experiments on ESEM and size distribution obtained by the GRIMM dust monitor were used in this study. The size distribution was found bi-modal in the winter and fall months and was primarily uni-modal during spring and summer. The aspect ratio for different filter samples collected inside the bus range from 2.4 to 3.6 in average value, with standard deviation ranging from 0.9 to 7.4. The square-shaped and oblong-shaped particles represent the single inhalable particle's morphology characteristics in the air of the Toledo transit buses. Copyright © 2011 Elsevier B.V. All rights reserved.

  10. Hollow Au/Ag nanostars displaying broad plasmonic resonance and high surface-enhanced Raman sensitivity

    NASA Astrophysics Data System (ADS)

    Garcia-Leis, Adianez; Torreggiani, Armida; Garcia-Ramos, Jose Vicente; Sanchez-Cortes, Santiago

    2015-08-01

    Bimetallic Au/Ag hollow nanostar (HNS) nanoparticles with different morphologies were prepared in this work. These nanoplatforms were obtained by changing the experimental conditions (concentration of silver and chemical reductors, hydroxylamine and citrate) and by using Ag nanostars as template nanoparticles (NPs) through galvanic replacement. The goal of this research was to create bimetallic Au/Ag star-shaped nanoparticles with advanced properties displaying a broader plasmonic resonance, a cleaner exposed surface, and a high concentration of electromagnetic hot spots on the surface provided by the special morphology of nanostars. The size, shape, and composition of Ag as well as their optical properties were studied by extinction spectroscopy, hyperspectral dark field microscopy, transmission and scanning electron microscopy (TEM and SEM), and energy dispersive X-ray spectroscopy (EDX). Finally, the surface-enhanced Raman scattering (SERS) activity of these HNS was investigated by using thioflavin T, a biomarker of the β-amyloid fibril formation, responsible for Alzheimer's disease. Lucigenin, a molecule displaying different SERS activities on Au and Ag, was also used to explore the presence of these metals on the NP surface. Thus, a relationship between the morphology, plasmon resonance and SERS activity of these new NPs was made.Bimetallic Au/Ag hollow nanostar (HNS) nanoparticles with different morphologies were prepared in this work. These nanoplatforms were obtained by changing the experimental conditions (concentration of silver and chemical reductors, hydroxylamine and citrate) and by using Ag nanostars as template nanoparticles (NPs) through galvanic replacement. The goal of this research was to create bimetallic Au/Ag star-shaped nanoparticles with advanced properties displaying a broader plasmonic resonance, a cleaner exposed surface, and a high concentration of electromagnetic hot spots on the surface provided by the special morphology of nanostars. The size, shape, and composition of Ag as well as their optical properties were studied by extinction spectroscopy, hyperspectral dark field microscopy, transmission and scanning electron microscopy (TEM and SEM), and energy dispersive X-ray spectroscopy (EDX). Finally, the surface-enhanced Raman scattering (SERS) activity of these HNS was investigated by using thioflavin T, a biomarker of the β-amyloid fibril formation, responsible for Alzheimer's disease. Lucigenin, a molecule displaying different SERS activities on Au and Ag, was also used to explore the presence of these metals on the NP surface. Thus, a relationship between the morphology, plasmon resonance and SERS activity of these new NPs was made. Electronic supplementary information (ESI) available: The SERS spectra of ThT on A-E samples are provided at two different excitations: 532 and 785 nm (Fig. S1). See DOI: 10.1039/c5nr02819a

  11. Extracting hurricane eye morphology from spaceborne SAR images using morphological analysis

    NASA Astrophysics Data System (ADS)

    Lee, Isabella K.; Shamsoddini, Ali; Li, Xiaofeng; Trinder, John C.; Li, Zeyu

    2016-07-01

    Hurricanes are among the most destructive global natural disasters. Thus recognizing and extracting their morphology is important for understanding their dynamics. Conventional optical sensors, due to cloud cover associated with hurricanes, cannot reveal the intense air-sea interaction occurring at the sea surface. In contrast, the unique capabilities of spaceborne synthetic aperture radar (SAR) data for cloud penetration, and its backscattering signal characteristics enable the extraction of the sea surface roughness. Therefore, SAR images enable the measurement of the size and shape of hurricane eyes, which reveal their evolution and strength. In this study, using six SAR hurricane images, we have developed a mathematical morphology method for automatically extracting the hurricane eyes from C-band SAR data. Skeleton pruning based on discrete skeleton evolution (DSE) was used to ensure global and local preservation of the hurricane eye shape. This distance weighted algorithm applied in a hierarchical structure for extraction of the edges of the hurricane eyes, can effectively avoid segmentation errors by reducing redundant skeletons attributed to speckle noise along the edges of the hurricane eye. As a consequence, the skeleton pruning has been accomplished without deficiencies in the key hurricane eye skeletons. A morphology-based analyses of the subsequent reconstructions of the hurricane eyes shows a high degree of agreement with the hurricane eye areas derived from reference data based on NOAA manual work.

  12. MO-C-17A-04: Forecasting Longitudinal Changes in Oropharyngeal Tumor Morphology Throughout the Course of Head and Neck Radiation Therapy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Yock, A; UT Graduate School of Biomedical Sciences, Houston, TX; Rao, A

    2014-06-15

    Purpose: To generate, evaluate, and compare models that predict longitudinal changes in tumor morphology throughout the course of radiation therapy. Methods: Two morphology feature vectors were used to describe the size, shape, and position of 35 oropharyngeal GTVs at each treatment fraction during intensity-modulated radiation therapy. The feature vectors comprised the coordinates of the GTV centroids and one of two shape descriptors. One shape descriptor was based on radial distances between the GTV centroid and 614 GTV surface landmarks. The other was based on a spherical harmonic decomposition of these distances. Feature vectors over the course of therapy were describedmore » using static, linear, and mean models. The error of these models in forecasting GTV morphology was evaluated with leave-one-out cross-validation, and their accuracy was compared using Wilcoxon signed-rank tests. The effect of adjusting model parameters at 1, 2, 3, or 5 time points (adjustment points) was also evaluated. Results: The addition of a single adjustment point to the static model decreased the median error in forecasting the position of GTV surface landmarks by 1.2 mm (p<0.001). Additional adjustment points further decreased forecast error by about 0.4 mm each. The linear model decreased forecast error compared to the static model for feature vectors based on both shape descriptors (0.2 mm), while the mean model did so only for those based on the inter-landmark distances (0.2 mm). The decrease in forecast error due to adding adjustment points was greater than that due to model selection. Both effects diminished with subsequent adjustment points. Conclusion: Models of tumor morphology that include information from prior patients and/or prior treatment fractions are able to predict the tumor surface at each treatment fraction during radiation therapy. The predicted tumor morphology can be compared with patient anatomy or dose distributions, opening the possibility of anticipatory re-planning. American Legion Auxiliary Fellowship; The University of Texas Graduate School of Biomedical Sciences at Houston.« less

  13. Towards a morphological metric of assemblage dynamics in the fossil record: a test case using planktonic foraminifera

    PubMed Central

    Hsiang, Allison Y.; Elder, Leanne E.; Hull, Pincelli M.

    2016-01-01

    With a glance, even the novice naturalist can tell you something about the ecology of a given ecosystem. This is because the morphology of individuals reflects their evolutionary history and ecology, and imparts a distinct ‘look’ to communities—making it possible to immediately discern between deserts and forests, or coral reefs and abyssal plains. Once quantified, morphology can provide a common metric for characterizing communities across space and time and, if measured rapidly, serve as a powerful tool for quantifying biotic dynamics. Here, we present and test a new high-throughput approach for analysing community shape in the fossil record using semi-three-dimensional (3D) morphometrics from vertically stacked images (light microscopic or photogrammetric). We assess the potential informativeness of community morphology in a first analysis of the relationship between 3D morphology, ecology and phylogeny in 16 extant species of planktonic foraminifera—an abundant group in the marine fossil record—and in a preliminary comparison of four assemblages from the North Atlantic. In the species examined, phylogenetic relatedness was most closely correlated with ecology, with all three ecological traits examined (depth habitat, symbiont ecology and biogeography) showing significant phylogenetic signal. By contrast, morphological trees (based on 3D shape similarity) were relatively distantly related to both ecology and phylogeny. Although improvements are needed to realize the full utility of community morphometrics, our approach already provides robust volumetric measurements of assemblage size, a key ecological characteristic. PMID:26977067

  14. Towards a morphological metric of assemblage dynamics in the fossil record: a test case using planktonic foraminifera.

    PubMed

    Hsiang, Allison Y; Elder, Leanne E; Hull, Pincelli M

    2016-04-05

    With a glance, even the novice naturalist can tell you something about the ecology of a given ecosystem. This is because the morphology of individuals reflects their evolutionary history and ecology, and imparts a distinct 'look' to communities--making it possible to immediately discern between deserts and forests, or coral reefs and abyssal plains. Once quantified, morphology can provide a common metric for characterizing communities across space and time and, if measured rapidly, serve as a powerful tool for quantifying biotic dynamics. Here, we present and test a new high-throughput approach for analysing community shape in the fossil record using semi-three-dimensional (3D) morphometrics from vertically stacked images (light microscopic or photogrammetric). We assess the potential informativeness of community morphology in a first analysis of the relationship between 3D morphology, ecology and phylogeny in 16 extant species of planktonic foraminifera--an abundant group in the marine fossil record--and in a preliminary comparison of four assemblages from the North Atlantic. In the species examined, phylogenetic relatedness was most closely correlated with ecology, with all three ecological traits examined (depth habitat, symbiont ecology and biogeography) showing significant phylogenetic signal. By contrast, morphological trees (based on 3D shape similarity) were relatively distantly related to both ecology and phylogeny. Although improvements are needed to realize the full utility of community morphometrics, our approach already provides robust volumetric measurements of assemblage size, a key ecological characteristic. © 2016 The Authors.

  15. Quantitative analysis of fetal facial morphology using 3D ultrasound and statistical shape modeling: a feasibility study.

    PubMed

    Dall'Asta, Andrea; Schievano, Silvia; Bruse, Jan L; Paramasivam, Gowrishankar; Kaihura, Christine Tita; Dunaway, David; Lees, Christoph C

    2017-07-01

    The antenatal detection of facial dysmorphism using 3-dimensional ultrasound may raise the suspicion of an underlying genetic condition but infrequently leads to a definitive antenatal diagnosis. Despite advances in array and noninvasive prenatal testing, not all genetic conditions can be ascertained from such testing. The aim of this study was to investigate the feasibility of quantitative assessment of fetal face features using prenatal 3-dimensional ultrasound volumes and statistical shape modeling. STUDY DESIGN: Thirteen normal and 7 abnormal stored 3-dimensional ultrasound fetal face volumes were analyzed, at a median gestation of 29 +4  weeks (25 +0 to 36 +1 ). The 20 3-dimensional surface meshes generated were aligned and served as input for a statistical shape model, which computed the mean 3-dimensional face shape and 3-dimensional shape variations using principal component analysis. Ten shape modes explained more than 90% of the total shape variability in the population. While the first mode accounted for overall size differences, the second highlighted shape feature changes from an overall proportionate toward a more asymmetric face shape with a wide prominent forehead and an undersized, posteriorly positioned chin. Analysis of the Mahalanobis distance in principal component analysis shape space suggested differences between normal and abnormal fetuses (median and interquartile range distance values, 7.31 ± 5.54 for the normal group vs 13.27 ± 9.82 for the abnormal group) (P = .056). This feasibility study demonstrates that objective characterization and quantification of fetal facial morphology is possible from 3-dimensional ultrasound. This technique has the potential to assist in utero diagnosis, particularly of rare conditions in which facial dysmorphology is a feature. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. The evolution of the avian bill as a thermoregulatory organ.

    PubMed

    Tattersall, Glenn J; Arnaout, Bassel; Symonds, Matthew R E

    2017-08-01

    The avian bill is a textbook example of how evolution shapes morphology in response to changing environments. Bills of seed-specialist finches in particular have been the focus of intense study demonstrating how climatic fluctuations acting on food availability drive bill size and shape. The avian bill also plays an important but under-appreciated role in body temperature regulation, and therefore in energetics. Birds are endothermic and rely on numerous mechanisms for balancing internal heat production with biophysical constraints of the environment. The bill is highly vascularised and heat exchange with the environment can vary substantially, ranging from around 2% to as high as 400% of basal heat production in certain species. This heat exchange may impact how birds respond to heat stress, substitute for evaporative water loss at elevated temperatures or environments of altered water availability, or be an energetic liability at low environmental temperatures. As a result, in numerous taxa, there is evidence for a positive association between bill size and environmental temperatures, both within and among species. Therefore, bill size is both developmentally flexible and evolutionarily adaptive in response to temperature. Understanding the evolution of variation in bill size however, requires explanations of all potential mechanisms. The purpose of this review, therefore, is to promote a greater understanding of the role of temperature on shaping bill size over spatial gradients as well as developmental, seasonal, and evolutionary timescales. © 2016 Cambridge Philosophical Society.

  17. Synthesis of Gold Nanoparticles with Buffer-Dependent Variations of Size and Morphology in Biological Buffers.

    PubMed

    Ahmed, Syed Rahin; Oh, Sangjin; Baba, Rina; Zhou, Hongjian; Hwang, Sungu; Lee, Jaebeom; Park, Enoch Y

    2016-12-01

    The demand for biologically compatible and stable noble metal nanoparticles (NPs) has increased in recent years due to their inert nature and unique optical properties. In this article, we present 11 different synthetic methods for obtaining gold nanoparticles (Au NPs) through the use of common biological buffers. The results demonstrate that the sizes, shapes, and monodispersity of the NPs could be varied depending on the type of buffer used, as these buffers acted as both a reducing agent and a stabilizer in each synthesis. Theoretical simulations and electrochemical experiments were performed to understand the buffer-dependent variations of size and morphology exhibited by these Au NPs, which revealed that surface interactions and the electrostatic energy on the (111) surface of Au were the determining factors. The long-term stability of the synthesized NPs in buffer solution was also investigated. Most NPs synthesized using buffers showed a uniquely wide range of pH stability and excellent cell viability without the need for further modifications.

  18. Characteristics of Non-Cohesive Embankment Failure

    NASA Astrophysics Data System (ADS)

    Yusof, Z. M.; Wahab, A. K. A.; Ismail, Z.; Amerudin, S.

    2018-04-01

    Embankments are important infrastructure built to provide flood control. They also present risks to property and life due to their potential to fail and cause catastrophic flooding. To mitigate these risks, authorities and regulators need to carefully analyse and inspect dams to identify potential failure modes and protect against them. This paper presents morphology of an embankment study and its sediment behaviour of different grain sizes after the embankment fails. A few experiments were carried out for the embankment size of 1V:3H with different sediment grain sizes; medium and coarser sand. The embankment material used is non-cohesive soil with the embankment height of 0.1 m. The embankment is tested with inflows rate of Q = 0.8 L/s. Experimental results showed the peak discharge for the same inflow rate is affected by the shape of embankment breached. The peak discharge of medium grain size of the embankment is highest, which gave 3.63 L/s in comparison with a coarser embankment. This concludes that the embankment morphology patterns are dissimilar to each other. The flow and dimension of embankment are shown to influence the characteristics of embankment failure.

  19. Controlling morphology and crystallite size of Cu(In{sub 0.7}Ga{sub 0.3})Se{sub 2} nano-crystals synthesized using a heating-up method

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hsu, Wei-Hsiang; Hsiang, Hsing-I, E-mail: hsingi@mail.ncku.edu.tw; Chia, Chih-Ta

    2013-12-15

    CuIn{sub 0.7}Ga{sub 0.3}Se{sub 2}(CIGS) nano-crystals were successfully synthesized via a heating-up process. The non-coordinating solvent (1-octadecene) and selenium/cations ratio effects on the crystalline phase and crystallite size of CIGS nano-crystallites were investigated. It was observed that the CIGS nano-crystallite morphology changed from sheet into spherical shape as the amount of 1-octadecene addition was increased. CIGS nano-crystals were obtained in 9–20 nm sizes as the selenium/cations ratio increased. These results suggest that the monomer reactivity in the solution can be adjusted by changing the solvent type and selenium/cations ratio, hence affecting the crystallite size and distribution. - Graphical abstract: CuIn{sub 0.7}Ga{submore » 0.3}Se{sub 2}(CIGS) nano-crystals were successfully synthesized via a heating-up process in this study. The super-saturation in the solution can be adjusted by changing the OLA/ODE ratio and selenium/cation ratio.« less

  20. Estimating the magnitude of morphoscapes: how to measure the morphological component of biodiversity in relation to habitats using geometric morphometrics

    NASA Astrophysics Data System (ADS)

    Fontaneto, Diego; Panisi, Martina; Mandrioli, Mauro; Montardi, Dario; Pavesi, Maurizio; Cardini, Andrea

    2017-08-01

    Ecological indicators are currently developed to account for the different facets of loss of biological diversity due to direct or indirect effects of human activities. Most ecological indicators include species richness as a metric. Others, such as functional traits and phylogenetic diversity, account for differences in species, even when species richness is the same. Here, we describe and apply a different indicator, called morphoscape dimension, accounting for morphological variability across habitats in a geographical region. We use the case of ground beetles (Coleoptera: Carabidae) in four different habitats in the Po Plain in Northern Italy to exemplify how to quantify the magnitude of the morphological space (i.e. the dimension of the morphoscape) occupied by the species in each habitat using geometric morphometrics. To this aim, we employed a variety of metrics of morphological disparity related to univariate size, and more complex multivariate shape and form. Our `proof of concept' suggests that metrics assessing size and form might largely tend to simply mirror the information provided by species richness, whereas shape morphoscape disparity may be able to account for non-trivial differences in species traits amongst habitats. This is indicated by the woodland morphoscape being on average bigger than that of crops, the most species-rich habitat, despite having almost 20% less species. We conclude suggesting that the analysis of morphoscape dimension has the potential to become a new additional and complimentary tool in the hands of conservation biologists and ecologists to explore and quantify habitat complexity and inform decisions on management and conservation based on a wide set of ecological indicators.

  1. Facile and rapid synthesis of Pd nanodendrites for electrocatalysis and surface-enhanced Raman scattering applications

    NASA Astrophysics Data System (ADS)

    Kannan, Palanisamy; Dolinska, Joanna; Maiyalagan, Thandavarayan; Opallo, Marcin

    2014-09-01

    Numerous properties from metal nanostructures can be tuned by controlling both their size and shape. In particular, the latter is extremely important because the type of crystalline surface affects the surface electronic density. This paper describes a simple approach to the synthesis of highly-structured, anisotropic palladium nanostructured dendrites. They were obtained using an eco-friendly biomolecule 5-hydroxytryptophan, which acts as both a reducing and stabilizing agent. The growth mechanism is proposed for the evolution of dendrites morphology. It was found that the concentration of 5-hydroxytryptophan played a vital role on the morphology of the nanostructured Pd dendrites. This nanomaterial shows enhanced electrocatalytic performance towards the oxidation of formic acid, and it exhibits surface-enhanced Raman scattering properties towards the prostate specific antigen. These properties may be explored in fuel cells and biosensors, respectively.Numerous properties from metal nanostructures can be tuned by controlling both their size and shape. In particular, the latter is extremely important because the type of crystalline surface affects the surface electronic density. This paper describes a simple approach to the synthesis of highly-structured, anisotropic palladium nanostructured dendrites. They were obtained using an eco-friendly biomolecule 5-hydroxytryptophan, which acts as both a reducing and stabilizing agent. The growth mechanism is proposed for the evolution of dendrites morphology. It was found that the concentration of 5-hydroxytryptophan played a vital role on the morphology of the nanostructured Pd dendrites. This nanomaterial shows enhanced electrocatalytic performance towards the oxidation of formic acid, and it exhibits surface-enhanced Raman scattering properties towards the prostate specific antigen. These properties may be explored in fuel cells and biosensors, respectively. Electronic supplementary information (ESI) available. See DOI: 10.1039/c4nr02896a

  2. Morphology informed by phylogeny reveals unexpected patterns of species differentiation in the aquatic moss Rhynchostegium riparioides s.l.

    PubMed

    Hutsemékers, Virginie; Vieira, Cristiana C; Ros, Rosa María; Huttunen, Sanna; Vanderpoorten, Alain

    2012-02-01

    Bryophyte floras typically exhibit extremely low levels of endemism. The interpretation, that this might reflect taxonomic shortcomings, is tested here for the Macaronesian flora, using the moss species complex of Rhynchostegium riparioides as a model. The deep polyphyly of R. riparioides across its distribution range reveals active differentiation that better corresponds to geographic than morphological differences. Morphometric analyses are, in fact, blurred by a size gradient that accounts for 80% of the variation observed among gametophytic traits. The lack of endemic diversification observed in R. riparioides in Macaronesia weakens the idea that the low rates of endemism observed in the Macaronesian bryophyte flora might solely be explained by taxonomic shortcomings. To the reverse, the striking polyphyly of North American and European lineages of R. riparioides suggests that the similarity between the floras of these continents has been over-emphasized. Discriminant analyses point to the existence of morphological discontinuities among the lineages resolved by the molecular phylogeny. The global rate of error associated to species identification based on morphology (0.23) indicates, however, that intergradation of shape and size characters among species in the group challenges their identification. Copyright © 2011 Elsevier Inc. All rights reserved.

  3. Smaller beaks for colder winters: Thermoregulation drives beak size evolution in Australasian songbirds.

    PubMed

    Friedman, Nicholas R; Harmáčková, Lenka; Economo, Evan P; Remeš, Vladimír

    2017-08-01

    Birds' beaks play a key role in foraging, and most research on their size and shape has focused on this function. Recent findings suggest that beaks may also be important for thermoregulation, and this may drive morphological evolution as predicted by Allen's rule. However, the role of thermoregulation in the evolution of beak size across species remains largely unexplored. In particular, it remains unclear whether the need for retaining heat in the winter or dissipating heat in the summer plays the greater role in selection for beak size. Comparative studies are needed to evaluate the relative importance of these functions in beak size evolution. We addressed this question in a clade of birds exhibiting wide variation in their climatic niche: the Australasian honeyeaters and allies (Meliphagoidea). Across 158 species, we compared species' climatic conditions extracted from their ranges to beak size measurements in a combined spatial-phylogenetic framework. We found that winter minimum temperature was positively correlated with beak size, while summer maximum temperature was not. This suggests that while diet and foraging behavior may drive evolutionary changes in beak shape, changes in beak size can also be explained by the beak's role in thermoregulation, and winter heat retention in particular. © 2017 The Author(s). Evolution © 2017 The Society for the Study of Evolution.

  4. Variation of the shape and morphological properties of silica and metal oxide powders by electro homogeneous precipitation

    DOEpatents

    Harris, M.T.; Basaran, O.A.; Sisson, W.G.; Brunson, R.R.

    1997-02-18

    The present invention provides a method for preparing irreversible linear aggregates (fibrils) of metal oxide powders by utilizing static or pulsed DC electrical fields across a relatively non-conducting liquid solvent in which organometal compounds or silicon alkoxides have been dissolved. The electric field is applied to the relatively non-conducting solution throughout the particle formation and growth process promoting the formation of either linear aggregates (fibrils) or spherical shaped particles as desired. Thus the present invention provides a physical method for altering the size, shape and porosity of precursor hydrous metal oxide or hydrous silicon oxide powders for the development of advanced ceramics with improved strength and insulating capacity. 3 figs.

  5. A Statistical Skull Geometry Model for Children 0-3 Years Old

    PubMed Central

    Li, Zhigang; Park, Byoung-Keon; Liu, Weiguo; Zhang, Jinhuan; Reed, Matthew P.; Rupp, Jonathan D.; Hoff, Carrie N.; Hu, Jingwen

    2015-01-01

    Head injury is the leading cause of fatality and long-term disability for children. Pediatric heads change rapidly in both size and shape during growth, especially for children under 3 years old (YO). To accurately assess the head injury risks for children, it is necessary to understand the geometry of the pediatric head and how morphologic features influence injury causation within the 0–3 YO population. In this study, head CT scans from fifty-six 0–3 YO children were used to develop a statistical model of pediatric skull geometry. Geometric features important for injury prediction, including skull size and shape, skull thickness and suture width, along with their variations among the sample population, were quantified through a series of image and statistical analyses. The size and shape of the pediatric skull change significantly with age and head circumference. The skull thickness and suture width vary with age, head circumference and location, which will have important effects on skull stiffness and injury prediction. The statistical geometry model developed in this study can provide a geometrical basis for future development of child anthropomorphic test devices and pediatric head finite element models. PMID:25992998

  6. A statistical skull geometry model for children 0-3 years old.

    PubMed

    Li, Zhigang; Park, Byoung-Keon; Liu, Weiguo; Zhang, Jinhuan; Reed, Matthew P; Rupp, Jonathan D; Hoff, Carrie N; Hu, Jingwen

    2015-01-01

    Head injury is the leading cause of fatality and long-term disability for children. Pediatric heads change rapidly in both size and shape during growth, especially for children under 3 years old (YO). To accurately assess the head injury risks for children, it is necessary to understand the geometry of the pediatric head and how morphologic features influence injury causation within the 0-3 YO population. In this study, head CT scans from fifty-six 0-3 YO children were used to develop a statistical model of pediatric skull geometry. Geometric features important for injury prediction, including skull size and shape, skull thickness and suture width, along with their variations among the sample population, were quantified through a series of image and statistical analyses. The size and shape of the pediatric skull change significantly with age and head circumference. The skull thickness and suture width vary with age, head circumference and location, which will have important effects on skull stiffness and injury prediction. The statistical geometry model developed in this study can provide a geometrical basis for future development of child anthropomorphic test devices and pediatric head finite element models.

  7. Investigation of the operating conditions to morphology evolution of β-L-glutamic acid during seeded cooling crystallization

    NASA Astrophysics Data System (ADS)

    Zhang, Fangkun; Liu, Tao; Huo, Yan; Guan, Runduo; Wang, Xue Z.

    2017-07-01

    In this paper the effects of operating conditions including cooling rate, initial supersaturation, and seeding temperature were investigated on the morphology evolution of β-L-glutamic acid (β-LGA) during seeded cooling crystallization. Based on the results of in-situ image acquisition of the crystal morphology evolution during the crystallization process, it was found that the crystal products tend to be plate-like or short rod-like under a slow cooling rate, low initial supersaturation, and low seeding temperature. In the opposite, the operating conditions of a faster cooling rate, higher initial supersaturation, and higher seeding temperature tend to produce long rod-like or needle-like crystals, and meanwhile, the length and width of crystal products will be increased together with a wider crystal size distribution (CSD). The aspect ratio of crystals, defined by the crystal length over width measured from in-situ or sample images, was taken as a shape index to analyze the crystal morphologies. Based on comparative analysis of the experimental results, guidelines on these operating conditions were given for obtaining the desired crystal shapes, along with the strategies for obtaining a narrower CSD for better product quality. Experimental verifications were performed to illustrate the proposed guidelines on the operating conditions for seeded cooling crystallization of LGA solution.

  8. Structure-Preserving Smoothing of Biomedical Images

    NASA Astrophysics Data System (ADS)

    Gil, Debora; Hernàndez-Sabaté, Aura; Burnat, Mireia; Jansen, Steven; Martínez-Villalta, Jordi

    Smoothing of biomedical images should preserve gray-level transitions between adjacent tissues, while restoring contours consistent with anatomical structures. Anisotropic diffusion operators are based on image appearance discontinuities (either local or contextual) and might fail at weak inter-tissue transitions. Meanwhile, the output of block-wise and morphological operations is prone to present a block structure due to the shape and size of the considered pixel neighborhood.

  9. Shape control VO2 nanorods prepared by soft chemistry and electrochemical method

    NASA Astrophysics Data System (ADS)

    Simo, A.; Sibanyoni, J.; Fuku, X.; Numan, N.; Omorogbe, S.; Maaza, M.

    2018-07-01

    "Bottom up" approach is of primary interest for chemistry and materials science because the fundamental building blocks are atoms. Thus colloidal chemical synthetic methods can be utilized to prepare uniform nanocrystals with controlled particle size. In the following work of study, thermochromic VO2 nanostructures were prepared by hydrothermal technique soft chemistry. We concentrate on solution phase synthetic methods that enable a proper shape and size control of metal oxide nanocrystals. Their structural properties were studied by Scanning Electron Microscopy (SEM), Fourier Transform IR (FTIR) and Differential Scanning Calorimetry (DSC). It is demonstrated that the surfactant assistance (NaOH) has great influence on the morphology-control of the material. Electrochemical properties of the nanospheres show good stability after 20 cycles and the surface diffusion coefficient was calculated to be 5 × 10-6 cm2 s-1.

  10. The role of ligands in coinage-metal nanoparticles for electronics

    PubMed Central

    Kanelidis, Ioannis

    2017-01-01

    Coinage-metal nanoparticles are key components of many printable electronic inks. They can be combined with polymers to form conductive composites and have been used as the basis of molecular electronic devices. This review summarizes the multidimensional role of surface ligands that cover their metal cores. Ligands not only passivate crystal facets and determine growth rates and shapes; they also affect size and colloidal stability. Particle shapes can be tuned via the ligand choice while ligand length, size, ω-functionalities, and chemical nature influence shelf-life and stability of nanoparticles in dispersions. When particles are deposited, ligands affect the electrical properties of the resulting film, the morphology of particle films, and the nature of the interfaces. The effects of the ligands on sintering, cross-linking, and self-assembly of particles in electronic materials are discussed. PMID:29259877

  11. 1D vs. 2D shape selectivity in the crystallization-driven self-assembly of polylactide block copolymers.

    PubMed

    Inam, Maria; Cambridge, Graeme; Pitto-Barry, Anaïs; Laker, Zachary P L; Wilson, Neil R; Mathers, Robert T; Dove, Andrew P; O'Reilly, Rachel K

    2017-06-01

    2D materials such as graphene, LAPONITE® clays or molybdenum disulfide nanosheets are of extremely high interest to the materials community as a result of their high surface area and controllable surface properties. While several methods to access 2D inorganic materials are known, the investigation of 2D organic nanomaterials is less well developed on account of the lack of ready synthetic accessibility. Crystallization-driven self-assembly (CDSA) has become a powerful method to access a wide range of complex but precisely-defined nanostructures. The preparation of 2D structures, however, particularly those aimed towards biomedical applications, is limited, with few offering biocompatible and biodegradable characteristics as well as control over self-assembly in two dimensions. Herein, in contrast to conventional self-assembly rules, we show that the solubility of polylactide (PLLA)-based amphiphiles in alcohols results in unprecedented shape selectivity based on unimer solubility. We use log  P oct analysis to drive solvent selection for the formation of large uniform 2D diamond-shaped platelets, up to several microns in size, using long, soluble coronal blocks. By contrast, less soluble PLLA-containing block copolymers yield cylindrical micelles and mixed morphologies. The methods developed in this work provide a simple and consistently reproducible protocol for the preparation of well-defined 2D organic nanomaterials, whose size and morphology are expected to facilitate potential applications in drug delivery, tissue engineering and in nanocomposites.

  12. Formulation of a dry powder influenza vaccine for nasal delivery.

    PubMed

    Garmise, Robert J; Mar, Kevin; Crowder, Timothy M; Hwang, C Robin; Ferriter, Matthew; Huang, Juan; Mikszta, John A; Sullivan, Vincent J; Hickey, Anthony J

    2006-03-10

    The purpose of this research was to prepare a dry powder vaccine formulation containing whole inactivated influenza virus (WIIV) and a mucoadhesive compound suitable for nasal delivery. Powders containing WIIV and either lactose or trehalose were produced by lyophilization. A micro-ball mill was used to reduce the lyophilized cake to sizes suitable for nasal delivery. Chitosan flakes were reduced in size using a cryo-milling technique. Milled powders were sieved between 45 and 125 microm aggregate sizes and characterized for particle size and distribution, morphology, and flow properties. Powders were blended in the micro-ball mill without the ball. Lyophilization followed by milling produced irregularly shaped, polydisperse particles with a median primary particle diameter of approximately 21 microm and a yield of approximately 37% of particles in the 45 to 125 microm particle size range. Flow properties of lactose and trehalose powders after lyophilization followed by milling and sieving were similar. Cryo-milling produced a small yield of particles in the desired size range (<10%). Lyophilization followed by milling and sieving produced particles suitable for nasal delivery with different physicochemical properties as a function of processing conditions and components of the formulation. Further optimization of particle size and morphology is required for these powders to be suitable for clinical evaluation.

  13. Conservatism and Adaptability during Squirrel Radiation: What Is Mandible Shape Telling Us?

    PubMed Central

    Casanovas-Vilar, Isaac; van Dam, Jan

    2013-01-01

    Both functional adaptation and phylogeny shape the morphology of taxa within clades. Herein we explore these two factors in an integrated way by analyzing shape and size variation in the mandible of extant squirrels using landmark-based geometric morphometrics in combination with a comparative phylogenetic analysis. Dietary specialization and locomotion were found to be reliable predictors of mandible shape, with the prediction by locomotion probably reflecting the underlying diet. In addition a weak but significant allometric effect could be demonstrated. Our results found a strong phylogenetic signal in the family as a whole as well as in the main clades, which is in agreement with the general notion of squirrels being a conservative group. This fact does not preclude functional explanations for mandible shape, but rather indicates that ancient adaptations kept a prominent role, with most genera having diverged little from their ancestral clade morphologies. Nevertheless, certain groups have evolved conspicuous adaptations that allow them to specialize on unique dietary resources. Such adaptations mostly occurred in the Callosciurinae and probably reflect their radiation into the numerous ecological niches of the tropical and subtropical forests of Southeastern Asia. Our dietary reconstruction for the oldest known fossil squirrels (Eocene, 36 million years ago) show a specialization on nuts and seeds, implying that the development from protrogomorphous to sciuromorphous skulls was not necessarily related to a change in diet. PMID:23593456

  14. Cranial shape transformation in the evolution of the giant panda ( Ailuropoda melanoleuca)

    NASA Astrophysics Data System (ADS)

    Figueirido, Borja; Palmqvist, Paul; Pérez-Claros, Juan A.; Dong, Wei

    2011-02-01

    In this study, landmark-based methods of geometric morphometrics are used for investigating the main aspects of cranial shape transformation in the evolution of the giant panda, Ailuropoda melanoleuca. Specifically, we explore if the highly derived cranial adaptations for bamboo feeding of the living panda were developed early in the panda's lineage. Results obtained show that the overall cranial morphologies of the oldest known panda, the "pygmy" Ailuropoda microta, and the late Pleistocene Ailuropoda baconi are both very similar to that of their closest living relative, A. melanoleuca, which agrees with a previous proposal based on qualitative criteria. However, we also describe several differences between the crania of A. microta, A. baconi, and A. melanoleuca, including the development of the postorbital process, the orientation of the occipital region, and the expansion of the braincase. As a result, the cranial morphology of A. microta shows a less specialized morphology toward a fibrous and durophagous diet compared to the giant panda. These results are confirmed by a comparative analysis of the dimensions of the upper teeth in bears, which has revealed differences in relative tooth size between A. microta and A. melanoleuca, most probably as a result of mosaic evolution. Therefore, we conclude that cranial shape did not remain essentially uniform in the Ailuropoda lineage, as previously thought, but underwent a number of changes during more than 2 Myr.

  15. Cranial shape transformation in the evolution of the giant panda (Ailuropoda melanoleuca).

    PubMed

    Figueirido, Borja; Palmqvist, Paul; Pérez-Claros, Juan A; Dong, Wei

    2011-02-01

    In this study, landmark-based methods of geometric morphometrics are used for investigating the main aspects of cranial shape transformation in the evolution of the giant panda, Ailuropoda melanoleuca. Specifically, we explore if the highly derived cranial adaptations for bamboo feeding of the living panda were developed early in the panda's lineage. Results obtained show that the overall cranial morphologies of the oldest known panda, the "pygmy" Ailuropoda microta, and the late Pleistocene Ailuropoda baconi are both very similar to that of their closest living relative, A. melanoleuca, which agrees with a previous proposal based on qualitative criteria. However, we also describe several differences between the crania of A. microta, A. baconi, and A. melanoleuca, including the development of the postorbital process, the orientation of the occipital region, and the expansion of the braincase. As a result, the cranial morphology of A. microta shows a less specialized morphology toward a fibrous and durophagous diet compared to the giant panda. These results are confirmed by a comparative analysis of the dimensions of the upper teeth in bears, which has revealed differences in relative tooth size between A. microta and A. melanoleuca, most probably as a result of mosaic evolution. Therefore, we conclude that cranial shape did not remain essentially uniform in the Ailuropoda lineage, as previously thought, but underwent a number of changes during more than 2 Myr.

  16. Extracellular biosynthesis of monodispersed gold nanoparticles by a SAM capping route

    NASA Astrophysics Data System (ADS)

    Wen, Li; Lin, Zhonghua; Gu, Pingying; Zhou, Jianzhang; Yao, Bingxing; Chen, Guoliang; Fu, Jinkun

    2009-02-01

    Monodispersed gold nanoparticles capped with a self-assembled monolayer of dodecanethiol were biosynthesized extracellularly by an efficient, simple, and environmental friendly procedure, which involved the use of Bacillus megatherium D01 as the reducing agent and the use of dodecanethiol as the capping ligand at 26 °C. The kinetics of gold nanoparticle formation was followed by transmission electron microscope (TEM) and UV-vis spectroscopy. It was shown that reaction time was an important parameter in controlling the morphology of gold nanoparticles. The effect of thiol on the shape, size, and dispersity of gold nanoparticles was also studied. The results showed that the presence of thiol during the biosynthesis could induce the formation of small size gold nanoparticles (<2.5 nm), hold the shape of spherical nanoparticles, and promote the monodispersity of nanoparticles. Through the modulation of reaction time and the use of thiol, monodispersed spherical gold nanoparticles capped with thiol of 1.9 ± 0.8 nm size were formed by using Bacillus megatherium D01.

  17. Synthesis of silver nanoparticles by silver salt reduction and its characterization

    NASA Astrophysics Data System (ADS)

    Muzamil, Muhammad; Khalid, Naveed; Danish Aziz, M.; Aun Abbas, S.

    2014-06-01

    The wet chemical method route by metal salt reduction has been used to synthesize nanoparticles, using silver nitrate as an inorganic salt, aldehyde as a reducing agent and amino acid as a catalyst. During the reaction aldehyde oxidizes to carboxylic acid and encapsulates the silver nanoparticles to prevent agglomeration and provide barrier in the growth of particle. The existing work produces particles using lab grade chemical, here the presented work is by using industrial grade chemicals to make the process more cost & time effective. The nano silver powder has been studied for their formation, particle size, shape & compositional analysis using Scanning Electron Microscope (SEM) equipped with EDS. The particles size distributions were analyzed by Laser Particle Analyzer (LPA), structure & morphological analysis using x-ray diffraction (XRD) and Fourier-transform-infrared Spectroscopy (FTIR) confirmed the stabilization of particles by coating of carboxylic group. These studies infer that the particles are mostly spherical in shape and have an average size between 70 to 350 nm.

  18. An experimental investigation of localised surface plasmon resonance (LSPR) for Cu nanoparticles depending as a function of laser pulse number in Pulsed Laser Deposition

    NASA Astrophysics Data System (ADS)

    Gezgin, Serap Yiǧit; Kepceoǧlu, Abdullah; Kılıç, Hamdi Şükür

    2017-02-01

    Copper is a low cost metal and its nanoparticles have a unique optical properties such as LSPR. The location of LSPR wavelength can be tuned by controlling nanoparticles sizes and size distributions of nanoparticles, shapes and interparticle distances. This morphological changes are provided by controlling system parameters in PLD. For this work, 48000 and 36000 laser pulses from Nd:YAG laser were applied to produce Cu nanoparticle thin films. These thin films were characterised by performing UV-VIS absorption spectroscopy, Atomic Force Microscopy (AFM) analysis. When the number of laser pulse decreases, the size of Cu nanoparticles and the number of nanoparticles arriving on the substrate are reduced, and LSPR peak of thin films are red shifted depending on the geometrical shapes of the Cu nanoparticles. We have driven a conclusion in this work that LSPR properties of Cu nanoparticles can be tuned by proposed method.

  19. Homogeneous Analysis of the Dust Morphology of Transition Disks Observed with ALMA: Investigating Dust Trapping and the Origin of the Cavities

    NASA Astrophysics Data System (ADS)

    Pinilla, P.; Tazzari, M.; Pascucci, I.; Youdin, A. N.; Garufi, A.; Manara, C. F.; Testi, L.; van der Plas, G.; Barenfeld, S. A.; Canovas, H.; Cox, E. G.; Hendler, N. P.; Pérez, L. M.; van der Marel, N.

    2018-05-01

    We analyze the dust morphology of 29 transition disks (TDs) observed with Atacama Large (sub-)Millimeter Array (ALMA) at (sub-)millimeter emission. We perform the analysis in the visibility plane to characterize the total flux, cavity size, and shape of the ring-like structure. First, we found that the M dust–M ⋆ relation is much flatter for TDs than the observed trends from samples of class II sources in different star-forming regions. This relation demonstrates that cavities open in high (dust) mass disks, independent of the stellar mass. The flatness of this relation contradicts the idea that TDs are a more evolved set of disks. Two potential reasons (not mutually exclusive) may explain this flat relation: the emission is optically thick or/and millimeter-sized particles are trapped in a pressure bump. Second, we discuss our results of the cavity size and ring width in the context of different physical processes for cavity formation. Photoevaporation is an unlikely leading mechanism for the origin of the cavity of any of the targets in the sample. Embedded giant planets or dead zones remain as potential explanations. Although both models predict correlations between the cavity size and the ring shape for different stellar and disk properties, we demonstrate that with the current resolution of the observations, it is difficult to obtain these correlations. Future observations with higher angular resolution observations of TDs with ALMA will help discern between different potential origins of cavities in TDs.

  20. Morphometry of terrestrial shield volcanoes

    NASA Astrophysics Data System (ADS)

    Grosse, Pablo; Kervyn, Matthieu

    2018-03-01

    Shield volcanoes are described as low-angle edifices built primarily by the accumulation of successive lava flows. This generic view of shield volcano morphology is based on a limited number of monogenetic shields from Iceland and Mexico, and a small set of large oceanic islands (Hawaii, Galápagos). Here, the morphometry of 158 monogenetic and polygenetic shield volcanoes is analyzed quantitatively from 90-meter resolution SRTM DEMs using the MORVOLC algorithm. An additional set of 24 lava-dominated 'shield-like' volcanoes, considered so far as stratovolcanoes, are documented for comparison. Results show that there is a large variation in shield size (volumes from 0.1 to > 1000 km3), profile shape (height/basal width (H/WB) ratios mostly from 0.01 to 0.1), flank slope gradients (average slopes mostly from 1° to 15°), elongation and summit truncation. Although there is no clear-cut morphometric difference between shield volcanoes and stratovolcanoes, an approximate threshold can be drawn at 12° average slope and 0.10 H/WB ratio. Principal component analysis of the obtained database enables to identify four key morphometric descriptors: size, steepness, plan shape and truncation. Hierarchical cluster analysis of these descriptors results in 12 end-member shield types, with intermediate cases defining a continuum of morphologies. The shield types can be linked in terms of growth stages and shape evolution, related to (1) magma composition and rheology, effusion rate and lava/pyroclast ratio, which will condition edifice steepness; (2) spatial distribution of vents, in turn related to the magmatic feeding system and the tectonic framework, which will control edifice plan shape; and (3) caldera formation, which will condition edifice truncation.

  1. Quantitative 3D shape description of dust particles from treated seeds by means of X-ray micro-CT.

    PubMed

    Devarrewaere, Wouter; Foqué, Dieter; Heimbach, Udo; Cantre, Dennis; Nicolai, Bart; Nuyttens, David; Verboven, Pieter

    2015-06-16

    Crop seeds are often treated with pesticides before planting. Pesticide-laden dust particles can be abraded from the seed coating during planting and expelled into the environment, damaging nontarget organisms. Drift of these dust particles depends on their size, shape and density. In this work, we used X-ray micro-CT to examine the size, shape (sphericity) and porosity of dust particles from treated seeds of various crops. The dust properties quantified in this work were very variable in different crops. This variability may be a result of seed morphology, seed batch, treatment composition, treatment technology, seed cleaning or an interaction of these factors. The intraparticle porosity of seed treatment dust particles varied from 0.02 to 0.51 according to the crop and generally increased with particle size. Calculated settling velocities demonstrated that accounting for particle shape and porosity is important in drift studies. For example, the settling velocity of dust particles with an equivalent diameter of 200 μm may vary between 0.1 and 1.2 m s(-1), depending on their shape and density. Our analysis shows that in a wind velocity of 5 m s(-1), such particles ejected at 1 m height may travel between 4 and 50 m from the source before settling. Although micro-CT is a valuable tool to characterize dust particles, the current image processing methodology limits the number of particles that can be analyzed.

  2. Influence of Size and Shape of Silica Supports on the Sol⁻Gel Surface Molecularly Imprinted Polymers for Selective Adsorption of Gossypol.

    PubMed

    Zhi, Keke; Wang, Lulu; Zhang, Yagang; Jiang, Yingfang; Zhang, Letao; Yasin, Akram

    2018-05-11

    The influence of various silica gel supports with different shapes and sizes on the recognition properties of surface molecular imprinted polymers (MIPs) was investigated. MIPs for selective recognition and adsorption of gossypol were synthesized via the sol⁻gel process with a surface imprinting technique on silica gel substrates. 3-aminopropyltriethoxysilane (APTES) and tetraethoxysilane (TEOS) were chosen as the functional monomer and the cross-linker. The morphology and structure of the gossypol-MIPs were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and a standard Brunauer⁻Emett⁻Teller (BET) analysis. Results indicated that the surface imprinted polymer layer facilitated the removal and rebinding of the template, and thus, achieved fast binding kinetics. Compared with the MIPs prepared on irregularly shaped silica with a broad particle size distribution, the MIPs using regularly-shaped silica of uniform size showed higher imprinting factor (IF), and the MIP made with a relatively larger sized (60 μm) spherical silica, demonstrated higher adsorption capacity compared to the MIPs made with smaller sized, spherical silica. The MIP prepared with 60 μm spherically shaped silica, featured a fast adsorption kinetic of 10 min, and a saturated adsorption capacity of 204 mg·g −1 . The gossypol-MIP had higher selectivity (IF = 2.20) for gossypol over its structurally-similar analogs ellagic acid (IF = 1.13) and quercetin (IF = 1.20). The adsorption data of the MIP correlated well with the pseudo-second-order kinetic model and the Freundlich isotherm model, which implied that chemical adsorption dominated, and that multilayer adsorption occurred. Furthermore, the MIP exhibited an excellent regeneration performance, and the adsorption capacity of the MIP for gossypol only decreased by 6% after six reused cycles, indicating good application potential for selective adsorption of gossypol.

  3. Morphology of the Physiological Apical Foramen in Maxillary and Mandibular First Molars

    PubMed Central

    Abarca, J.; Zaror, C.; Monardes, H.; Hermosilla, V.; Muñoz, C.; Cantin, M.

    2015-01-01

    SUMMARY Information regarding the anatomy of the physiological apical foramen is limited. Knowing its diameter and shapes contributes to clinical work, specifically to the cleaning and shaping of the apical third. The aim of this ex vivo study was to determine the minimum and maximum diameters and shape of the physiological apical foramen in the roots of maxillary and mandibular first molars. A descriptive study was conducted on 89 recently extracted first molars. Roots 3–5 mm from the apex were sectioned and prepared for analysis at 40× magnification. The minimum and maximum diameters of each physiological foramen were measured using the program Motic Images plus 2.0 ML. The shape of the foramina, classified as round, oval or irregular, was determined by the difference between the maximum and minimum diameters. A total of 174 physiological foramina were analyzed. The average of the minimum and maximum diameters was between 0.24–0.33 mm in maxillary first molars and between 0.25–0.33 mm in mandibular first molars. In maxillary molars, the most common shape of the foramen was oval (50%), then irregular (32%), then round (18%). In mandibular molars, the oval shape was also the most frequent (59%), followed by irregular (23%) and round (18%). The findings of this study regarding the morphology of physiological apical foramina in first molars make it easier for the operator to choose the appropriately-sized instruments to perform endodontic therapy successfully. PMID:25937698

  4. Morphology of the Physiological Apical Foramen in Maxillary and Mandibular First Molars.

    PubMed

    Abarca, J; Zaror, C; Monardes, H; Hermosilla, V; Muñoz, C; Cantin, M

    2014-06-01

    Information regarding the anatomy of the physiological apical foramen is limited. Knowing its diameter and shapes contributes to clinical work, specifically to the cleaning and shaping of the apical third. The aim of this ex vivo study was to determine the minimum and maximum diameters and shape of the physiological apical foramen in the roots of maxillary and mandibular first molars. A descriptive study was conducted on 89 recently extracted first molars. Roots 3-5 mm from the apex were sectioned and prepared for analysis at 40× magnification. The minimum and maximum diameters of each physiological foramen were measured using the program Motic Images plus 2.0 ML. The shape of the foramina, classified as round, oval or irregular, was determined by the difference between the maximum and minimum diameters. A total of 174 physiological foramina were analyzed. The average of the minimum and maximum diameters was between 0.24-0.33 mm in maxillary first molars and between 0.25-0.33 mm in mandibular first molars. In maxillary molars, the most common shape of the foramen was oval (50%), then irregular (32%), then round (18%). In mandibular molars, the oval shape was also the most frequent (59%), followed by irregular (23%) and round (18%). The findings of this study regarding the morphology of physiological apical foramina in first molars make it easier for the operator to choose the appropriately-sized instruments to perform endodontic therapy successfully.

  5. On the morphometry of terrestrial shield volcanoes

    NASA Astrophysics Data System (ADS)

    Grosse, Pablo; Kervyn, Matthieu

    2016-04-01

    Shield volcanoes are described as low angle edifices that have convex up topographic profiles and are built primarily by the accumulation of lava flows. This generic view of shields' morphology is based on a limited number of monogenetic shields from Iceland and Mexico, and a small set of large oceanic islands (Hawaii, Galapagos). Here, the morphometry of over 150 monogenetic and polygenetic shield volcanoes, identified inthe Global Volcanism Network database, are analysed quantitatively from 90-meter resolution DEMs using the MORVOLC algorithm. An additional set of 20 volcanoes identified as stratovolcanoes but having low slopes and being dominantly built up by accumulation of lava flows are documented for comparison. Results show that there is a large variation in shield size (volumes range from 0.1 to >1000 km3), profile shape (height/basal width ratios range from 0.01 to 0.1), flank slope gradients, elongation and summit truncation. Correlation and principal component analysis of the obtained quantitative database enables to identify 4 key morphometric descriptors: size, steepness, plan shape and truncation. Using these descriptors through clustering analysis, a new classification scheme is proposed. It highlights the control of the magma feeding system - either central, along a linear structure, or spatially diffuse - on the resulting shield volcano morphology. Genetic relationships and evolutionary trends between contrasted morphological end-members can be highlighted within this new scheme. Additional findings are that the Galapagos-type morphology with a central deep caldera and steep upper flanks are characteristic of other shields. A series of large oceanic shields have slopes systematically much steeper than the low gradients (<4-8°) generally attributed to large Hawaiian-type shields. Finally, the continuum of morphologies from flat shields to steeper complex volcanic constructs considered as stratovolcanoes calls for a revision of this oversimplified distinction, taking into account the lava/pyroclasts ratio and the spatial distribution of eruptive vents.

  6. Morphological and behavioral differences in the gastropod Trophon geversianus associated to distinct environmental conditions, as revealed by a multidisciplinary approach

    NASA Astrophysics Data System (ADS)

    Márquez, Federico; Nieto Vilela, Rocío Aimé; Lozada, Mariana; Bigatti, Gregorio

    2015-01-01

    The gastropod Trophon geversianus exhibits shell polymorphisms along its distribution in subtidal and intertidal habitats. Our hypothesis is that morphological and behavioral patterns of T. geversianus represent habitat-specific constrains; subsequently we expect an association between shell morphology, attachment behavior, and habitat. In order to test this hypothesis we compared individuals from intertidal and subtidal habitats, at three sites in Golfo Nuevo (Argentina). We analyzed shell morphology using classic morphometric variables, 3D geometric morphometrics and computing tomography scan. The results were complemented with field observations of attachment to substrate and turning time behavior, as well as of the number of shell scars produced by crab predation. Our results showed differences in shell size and shape between intertidal and subtidal-collected individuals. Centroid size, total weight and shell weight, as well as shell density and thickness were significantly lower in intertidal individuals than in subtidal ones. Gastropods from intertidal habitats presented a low-spired shell and an expanded aperture which might allow better attachment to the bottom substrate, while subtidal individuals presented a slender and narrower shell shape. The number of crab scars was significantly higher in shells from subtidal individuals. Observations of the behavior of gastropods placed at the intertidal splash zone showed 100% of attachment to the bottom in the intertidal individuals, while subtidal specimens only attached in average in 32% of the cases. These latter took 12 times longer to re-attach to the bottom when faced up. Phylogenetic analysis of COI gene fragments showed no consistent differences among individuals sampled in both habitats. All these results suggest that T. geversianus has developed two ecomorphs with distinct morphological and behavioral responses to physically stressful conditions registered in north Patagonian intertidals, as opposed to lower physical stress but higher predation pressure in the subtidal habitats. The findings of this work constitute a starting point in the study of ecological adaptation processes in gastropods from Patagonian coastal environments.

  7. Illustrating ontogenetic change in the dentition of the Nile monitor lizard, Varanus niloticus: a case study in the application of geometric morphometric methods for the quantification of shape-size heterodonty.

    PubMed

    D'Amore, Domenic C

    2015-05-01

    Many recent attempts have been made to quantify heterodonty in non-mammalian vertebrates, but the majority of these are limited to Euclidian measurements. One taxon frequently investigated is Varanus niloticus, the Nile monitor. Juveniles possess elongate, pointed teeth (caniniform) along the entirety of the dental arcade, whereas adults develop large, bulbous distal teeth (molariform). The purpose of this study was to present a geometric morphometric method to quantify V. niloticus heterodonty through ontogeny that may be applied to other non-mammalian taxa. Data were collected from the entire tooth row of 19 dry skull specimens. A semilandmark analysis was conducted on the outline of the photographed teeth, and size and shape were derived. Width was also measured with calipers. From these measures, sample ranges and allometric functions were created using multivariate statistical analyses for each tooth position separately, as well as overall measures of heterodonty for each specimen based on morphological disparity. The results confirm and expand upon previous studies, showing measurable shape-size heterodonty in the species with significant differences at each tooth position. Tooth size increases with body size at most positions, and the allometric coefficient increases at more distal positions. Width shows a dramatic increase at the distal positions with ontogeny, often displaying pronounced positive allometry. Dental shape varied in two noticeable ways, with the first composing the vast majority of shape variance: (i) caniniformy vs. molariformy and (ii) mesially leaning, 'rounded' apices vs. distally leaning, 'pointed' apices. The latter was twice as influential in the mandible, a consequence of host bone shape. Mesial teeth show no significant shape change with growth, whereas distal teeth change significantly due primarily to an increase in molariformy. Overall, heterodonty increases with body size concerning both tooth size and shape, but shape heterodonty changes in the mandible are much less pronounced. Although it is unclear to what degree V. niloticus specializes in hard prey items (durophagy), previous studies of varanid feeding behavior, along with research on analogous durophagous vertebrates, indicate a division of labor along the tooth row in adults, due to a possible transition to at least a partial durophagous niche. The geometric morphometric method proposed here, although not without its own limitations, may be ideal for use with a number of dental morphotypes in the future. © 2015 Anatomical Society.

  8. Shape-engineering substrate-based plasmonic nanomaterials

    NASA Astrophysics Data System (ADS)

    Gilroy, Kyle D.

    The advancement of next generation technologies is reliant on our ability to engineer matter at the nanoscale. Since the morphological features of nanomaterials dictate their chemical and physical properties, a significant effort has been put forth to develop syntheses aimed at fine tuning their size, shape and composition. This massive effort has resulted in a maturing colloidal chemistry containing an extensive collection of morphologies with compositions nearly spanning the entire transition of the periodic table. While colloidal nanoparticles have opened the door to promising applications in fields such as cancer theranostics, drug delivery, catalysis and sensing; the synthetic protocols for the placement of nanomaterials on surfaces, a requisite for chip-based devices, are ill-developed. This dissertation serves to address this limitation by highlighting a series of syntheses related to the design of substrate-based nanoparticles whose size, shape and composition are controllably engineered to a desired endpoint. The experimental methods are based on a template-mediated approach which sees chemical modifications made to prepositioned thermally assembled metal nanostructures which are well bonded to a sapphire substrate. The first series of investigations will highlight synthetic routes utilizing galvanic replacement reactions, where the prepositioned templates are chemically transformed into hollow nanoshells. Detailed studies are provided highlighting discoveries related to (i) hollowing, (ii) defect transfer, (iii) strain induction, (iv) interdiffusion, (v) crystal structure and (vi) the localized surface plasmon resonance (LSPR). The second series of investigations, based on heterogeneous nucleation, have Au templates serve as nucleation sites for metal atoms arriving in either the solution- or vapor phase. The solution-phase heterogeneous nucleation of Ag on Au reveals that chemical kinetics (injection rate & precursor concentration) can be used to control the nature of how Ag atoms grow on the Au template. It was discovered that (i) slow kinetics leads to an anisotropic growth mode (heterodimeric structures), (ii) fast kinetics causes a very uniform deposition (Au-Ag coreshell morphology, or Au Ag) and (iii) medium kinetics produces structures with an intermediate morphology (truncated octahedron). In the second case, where the nucleation event is carried out at high temperatures, the Ag vapor is sourced from a sublimating foil onto adjacent Au templates. This process drives the composition and morphology from a Au Wulff-shape to a homogeneous Au-Ag nanoprism. By tracking over time the (i) morphological features, (ii) LSPR and (iii) composition; insights into the fundamental atomic scale growth mechanisms are elucidated. Overall, substrate-based template-mediated syntheses have proven to be an effective route for directing growth pathways toward a desired endpoint giving rise to an impressive new group of complex substrate-based nanostructures with asymmetric, core-shell and hollowed morphologies. While this dissertation is focused heavily on the development of synthetic procedures aimed at generating substrate-based plasmonic nanomaterials, the last chapter will serve to highlight a series of on-going studies aimed at defining these nanomaterials as highly effective heterogeneous catalysts. Several examples are shown including (i) nanoparticle films synthesize via sputter deposition, (ii) mechanically induced nanotexturing of bulk copper foils, (iii) ultra-small AuPd nanoparticles synthesized via pulse laser, (iv) substrate-based AuCu nanoprisms and (v) the Wulff in a Cage Morphology.

  9. Effect of size and shape dependent anisotropy on superparamagnetic property of CoFe2O4 nanoparticles and nanoplatelets

    NASA Astrophysics Data System (ADS)

    Chandekar, Kamlesh V.; Kant, K. Mohan

    2017-09-01

    Superparamagnetic cobalt ferrite (CoFe2O4) spherical nanoparticles and rhomboidal nanoplatelets were synthesized by co-precipitation at 80 °C (S1) and hydrothermal route at 150 °C (S2). X-ray diffraction (XRD) pattern confirms formation of cubic inverse spinel structure of as prepared cobalt ferrite samples (S1 and S2) with average crystallite size of 13 nm and 18.7 nm for S1 and S2 respectively. Transmission electron microscopy (TEM) reveals spherical and rhomboidal shaped with average particle size 16.7 nm (S1) and 19.8 nm (S2). The zero field cooled magnetization MZFCvs. T exhibit a broad maxima at 400 K and 510 K for S1 and S2 respectively. The blocking temperature TB is obtained as 310 K and 341 K for S1 and S2 respectively, by fitting coercive field at different temperatures to T 1 / 2 law. The morphology of S1 and S2 corresponds to shape dependence of continuum approach. The effective demagnetization factors estimated as ΔN1 = 0 and ΔN2 = 0 . 749 for S1 and S2 samples respectively. The uniaxial anisotropy and shape anisotropy observed to be dominant in spherical shaped and rhomboidal shaped CoFe2O4 nanoparticles respectively. The uniaxial anisotropy constant of S1 sample is estimated as 56 (kJ/m3) at TB = 310 K whereas the effective anisotropy constant for S2 sample is 627 (kJ/m3) at TB = 341 K , in which shape anisotropy constant 605 (kJ/m3) dominates over contribution from uniaxial anisotropy constant 22 (kJ/m3) in S2 sample.

  10. Measurement, variation, and scaling of osteocyte lacunae: a case study in birds.

    PubMed

    D'Emic, Michael D; Benson, Roger B J

    2013-11-01

    Basic issues surrounding osteocyte biology are still poorly understood, including the variability of osteocyte morphology within and among bones, individuals, and species. Several studies have suggested that the volume or shape of osteocytes (or their lacunae) is related to bone and/or organismal growth rate or metabolism, but the nature of this relationship, if any, is unclear. Furthermore, several studies have linked osteocyte lacuna volume with genome size or growth rate and suggested that osteocyte lacuna volume is unrelated to body size. Herein the scaling of osteocyte lacuna volume with body mass, growth and basal metabolic rates, genome size, and red blood cell size is examined using a broad sample of extant birds within a phylogenetic framework. Over 12,000 osteocyte lacuna axes were measured in a variety of bones from 34 avian and four non-avian dinosaur species. Osteocyte lacunae in parallel-fibered bone are scalene ellipsoids; their morphology and volume cannot be reliably estimated from any single thin section, and using a prolate ellipsoid model to estimate osteocyte lacuna volume results in a substantial (ca. 2-7 times) underestimate relative to true lacunar volume. Orthogonal thin sections reveal that in birds, even when only observing parallel-fibered, primary, cortical bone, intra-skeletal variation in osteocyte lacuna volume and shape is very high (volumes vary by a factor of 5.4 among different bones), whereas variation among homologous bones of the same species is low (1.2-44%; mean=12%). Ordinary and phylogenetically informed bivariate and multiple regressions demonstrate that in birds, osteocyte volume scales significantly but weakly with body mass and mass-specific basal metabolic rate and moderately with genome size, but not with erythrocyte size. Avian whole-body growth rate and osteocyte lacuna volume are weakly and inversely related. Finally, we present the first three-dimensionally calculated osteocyte volumes for several non-avian dinosaurs, which are much larger than previously reported values and smaller than those of large extant avians. Osteocyte volumes estimated from a single transverse section and assuming prolate morphology, as done in previous studies, are relative underestimates in theropod dinosaurs compared to sauropod dinosaurs, raising the possibility that no major change in osteocyte volumes (and genome size) occurred within Theropoda on the lineage leading to birds. Osteocyte volume is intertwined with several organismal attributes whose relative importance varies at a number of hierarchical levels. © 2013.

  11. Actively targeted delivery of anticancer drug to tumor cells by redox-responsive star-shaped micelles.

    PubMed

    Shi, Chunli; Guo, Xing; Qu, Qianqian; Tang, Zhaomin; Wang, Yi; Zhou, Shaobing

    2014-10-01

    In cancer therapy nanocargos based on star-shaped polymer exhibit unique features such as better stability, smaller size distribution and higher drug capacity in comparison to linear polymeric micelles. In this study, we developed a multifunctional star-shaped micellar system by combination of active targeting ability and redox-responsive behavior. The star-shaped micelles with good stability were self-assembled from four-arm poly(ε-caprolactone)-poly(ethylene glycol) copolymer. The redox-responsive behaviors of these micelles triggered by glutathione were evaluated from the changes of micellar size, morphology and molecular weight. In vitro drug release profiles exhibited that in a stimulated normal physiological environment, the redox-responsive star-shaped micelles could maintain good stability, whereas in a reducing and acid environment similar with that of tumor cells, the encapsulated agent was promptly released. In vitro cellular uptake and subcellular localization of these micelles were further studied with confocal laser scanning microscopy and flow cytometry against the human cervical cancer cell line HeLa. In vivo and ex vivo DOX fluorescence imaging displayed that these FA-functionalized star-shaped micelles possessed much better specificity to target solid tumor. Both the qualitative and quantitative results of the antitumor effect in 4T1 tumor-bearing BALB/c mice demonstrated that these redox-responsive star-shaped micelles have a high therapeutic efficiency to artificial solid tumor. Therefore, the multifunctional star-shaped micelles are a potential platform for targeted anticancer drug delivery. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Co(II)-doped MOF-5 nano/microcrystals: Solvatochromic behaviour, sensing solvent molecules and gas sorption property

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Yang, Ji-Min; School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005; Liu, Qing

    2014-10-15

    Co(II)-doped MOF-5 nano/microcrystals with controllable morphology and size were successfully obtained by solvothermal method. The products were characterized by powder X-ray diffraction (PXRD), energy dispersive spectrometry (EDS), field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TGA), inductively coupled plasma optical emission spectrometer (ICP-OES), elemental analysis, UV–vis and infrared (IR) spectroscopy. The factors influencing the crystal morphology and size were investigated. The gas sorption measurements reveal that highly crystalline particles have large Langmuir surface area. It was found that the Co(II)-doped MOF-5 shows enhanced hydrostability and the sorption profiles of the Co(II)-doped MOF-5 nano/microcrystals are dependent on the morphology and sizemore » of the particles. Porous Co(II)-doped MOF-5 is stable upon the removal of guest molecules and exhibits different colour with accommodating different solvent molecule, which means that it can act as solvatochromic sensing materials for recognition of solvent molecules. - Graphical abstract: Co(II)-doped MOF-5 nano/microcrystals with different shapes and sizes were synthesized by a facile hydrothermal method, which not only enhance gas sorption properties and structural stability of MOFs towards moisture, but also act as new sensing materials for sensing small molecules. - Highlights: • Co(II)-doped MOF-5 nano/microcrystals with controllable morphology and size were obtained. • Co(II)-doped MOF-5 nano/microcrystals enhance the structural stability towards moisture. • Co(II)-doped MOF-5 can act as new sensing material for sensing small molecules.« less

  13. Quantum dot solar cells. Tuning photoresponse through size and shape control of CdSe-TiO2 architecture.

    PubMed

    Kongkanand, Anusorn; Tvrdy, Kevin; Takechi, Kensuke; Kuno, Masaru; Kamat, Prashant V

    2008-03-26

    Different-sized CdSe quantum dots have been assembled on TiO2 films composed of particle and nanotube morphologies using a bifunctional linker molecule. Upon band-gap excitation, CdSe quantum dots inject electrons into TiO2 nanoparticles and nanotubes, thus enabling the generation of photocurrent in a photoelectrochemical solar cell. The results presented in this study highlight two major findings: (i) ability to tune the photoelectrochemical response and photoconversion efficiency via size control of CdSe quantum dots and (ii) improvement in the photoconversion efficiency by facilitating the charge transport through TiO2 nanotube architecture. The maximum IPCE (photon-to-charge carrier generation efficiency) obtained with 3 nm diameter CdSe nanoparticles was 35% for particulate TiO2 and 45% for tubular TiO2 morphology. The maximum IPCE observed at the excitonic band increases with decreasing particle size, whereas the shift in the conduction band to more negative potentials increases the driving force and favors fast electron injection. The maximum power-conversion efficiency

  14. A remote sensing study of regional variation in sinkhole morphology-Florida karst vs. Minnesota karst

    NASA Astrophysics Data System (ADS)

    Ernst, C. L.; Hadizadeh, J.; McCarty, J. L.

    2010-12-01

    In many regions of the United States, database technologies and GIS have facilitated spatial analysis of karst. The purpose of this research was to compare regional latitudinal variation in sinkhole karst morphology via remote sensing techniques. Such comparison may be significant because the development of a karst landscape depends primarily on climate and availability of water as well as lithology. Sinkhole karst, a common karst in the U.S., is morphologically defined as cone-shaped depressions with circular or oval opening to the surface that result from the dissolution of relatively soluble bedrock such as limestone or gypsum. The two regions of interest, north-central Florida and southeastern Minnesota, were selected based on structural and lithological similarity of limestone bedrock and the fact that the bedrock study areas are located in clearly different climate zones. This approach utilized topographic maps, digital elevation models, state karst feature databases, and high resolution 0.6m QuickBirdTM and 0.5m WorldView 1TM satellite images in a GIS environment. Morphological parameters - area, perimeter, minor axis and major axis length - were calculated on a total of 80 sinkholes in the study regions using the zonal geometry function, a tool in the spatial analysis extension provided by ESRITM. Our results show that north-central Florida and southeastern Minnesota karst are statistically different in terms of sinkhole shape and size distribution. Florida has larger sinkholes (2,835 square meter Mean) that are closer to circular shape. Minnesota has smaller (1,213 square meter Mean) and more elliptical sinkholes with a comparatively shorter minor axis. Of the possible explanations, climate appears to be the most likely cause for the observed differences. The higher amount of precipitation in Florida coupled with warmer year round temperatures provides an environment conducive to a more chemically involved hydrological regime, which may be responsible for the greater average sinkhole size in Florida. The integration of high resolution satellite imagery was successfully implemented to delineate sinkhole features. There were several advantages to using QuickBirdTM and WorldView1TM satellite images such as easy integration into GIS, regional coverage and high spatial resolution.

  15. Ecological explanations to island gigantism: dietary niche divergence, predation, and size in an endemic lizard.

    PubMed

    Runemark, Anna; Sagonas, Kostas; Svensson, Erik I

    2015-08-01

    Although rapid evolution of body size on islands has long been known, the ecological mechanisms behind this island phenomenon remain poorly understood. Diet is an important selective pressure for morphological divergence. Here we investigate if selection for novel diets has contributed to the multiple independent cases of island gigantism in the Skyros wall lizard (Podarcis gaigeae) and if diet, predation, or both factors best explain island gigantism. We combined data on body size, shape, bite force, and realized and available diets to address this. Several lines of evidence suggest that diet has contributed to the island gigantism. The larger islet lizards have relatively wider heads and higher bite performance in relation to mainland lizards than would be expected from size differences alone. The proportions of consumed and available hard prey are higher on islets than mainland localities, and lizard body size is significantly correlated with the proportion of hard prey. Furthermore, the main axis of divergence in head shape is significantly correlated with dietary divergence. Finally, a model with only diet and one including diet and predation regime explain body size divergence equally well. Our results suggest that diet is an important ecological factor behind insular body size divergence, but could be consistent with an additional role for predation.

  16. Phalangeal morphology of Shanghuang fossil primates.

    PubMed

    Gebo, Daniel L; Dagosto, Marian; Ni, Xijun; Beard, K Christopher

    2017-12-01

    Here, we describe hundreds of isolated phalanges attributed to middle Eocene fossil primates from the Shanghuang fissure-fillings from southern Jiangsu Province, China. Extending knowledge based on previous descriptions of postcranial material from Shanghuang, this sample of primate finger and toe bones includes proximal phalanges, middle phalanges, and over three hundred nail-bearing distal phalanges. Most of the isolated proximal and middle phalanges fall within the range of small-bodied individuals, suggesting an allocation to the smaller haplorhine primates identified at Shanghuang, including eosimiids. In contrast to the proximal and middle phalanges from Shanghuang, there are a variety of shapes, sizes, and possible taxonomic allocations for the distal phalanges. Two distal phalangeal morphologies are numerically predominant at Shanghuang. The sample of larger bodied specimens is best allocated to the medium-sized adapiform Adapoides while the smaller ones are allocated to eosimiids on the basis of the commonality of dental and tarsal remains of these taxa at Shanghuang. The digit morphology of Adapoides is similar morphologically to that of notharctines and cercamoniines, while eosimiid digit morphology is unlike living anthropoids. Other primate distal phalangeal morphologies at Shanghuang include grooming "claws" as well as specimens attributable to tarsiids, tarsiiforms, the genus Macrotarsius, and a variety of adapiforms. One group of distal phalanges at Shanghuang is morphologically indistinguishable from those of living anthropoids. All of the phalanges suggest long fingers and toes for the fossil primates of Shanghaung, and their digit morphology implies arboreality with well-developed digital flexion and strong, grasping hands and feet. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Interactions among mechanisms of sexual selection on male body size and head shape in a sexually dimorphic fly.

    PubMed

    Bonduriansky, Russell; Rowe, Locke

    2003-09-01

    Darwin envisaged male-male and male-female interactions as mutually supporting mechanisms of sexual selection, in which the best armed males were also the most attractive to females. Although this belief continues to predominate today, it has been challenged by sexual conflict theory, which suggests that divergence in the interests of males and females may result in conflicting sexual selection. This raises the empirical question of how multiple mechanisms of sexual selection interact to shape targeted traits. We investigated sexual selection on male morphology in the sexually dimorphic fly Prochyliza xanthostoma, using indices of male performance in male-male and male-female interactions in laboratory arenas to calculate gradients of direct, linear selection on male body size and an index of head elongation. In male-male combat, the first interaction with a new opponent selected for large body size but reduced head elongation, whereas multiple interactions with the same opponent favored large body size only. In male-female interactions, females preferred males with relatively elongated heads, but male performance of the precopulatory leap favored large body size and, possibly, reduced head elongation. In addition, the amount of sperm transferred (much of which is ingested by females) was an increasing function of both body size and head elongation. Thus, whereas both male-male and male-female interactions favored large male body size, male head shape appeared to be subject to conflicting sexual selection. We argue that conflicting sexual selection may be a common result of divergence in the interests of the sexes.

  18. Chewing on the trees: Constraints and adaptation in the evolution of the primate mandible.

    PubMed

    Meloro, Carlo; Cáceres, Nilton Carlos; Carotenuto, Francesco; Sponchiado, Jonas; Melo, Geruza Leal; Passaro, Federico; Raia, Pasquale

    2015-07-01

    Chewing on different food types is a demanding biological function. The classic assumption in studying the shape of feeding apparatuses is that animals are what they eat, meaning that adaptation to different food items accounts for most of their interspecific variation. Yet, a growing body of evidence points against this concept. We use the primate mandible as a model structure to investigate the complex interplay among shape, size, diet, and phylogeny. We find a weak but significant impact of diet on mandible shape variation in primates as a whole but not in anthropoids and catarrhines as tested in isolation. These clades mainly exhibit allometric shape changes, which are unrelated to diet. Diet is an important factor in the diversification of strepsirrhines and platyrrhines and a phylogenetic signal is detected in all primate clades. Peaks in morphological disparity occur during the Oligocene (between 37 and 25 Ma) supporting the notion that an adaptive radiation characterized the evolution of South American monkeys. In all primate clades, the evolution of mandible size is faster than its shape pointing to a strong effect of allometry on ecomorphological diversification in this group. © 2015 The Author(s). Evolution © 2015 The Society for the Study of Evolution.

  19. The ontogenetic origins of skull shape disparity in the Triturus cristatus group.

    PubMed

    Cvijanović, Milena; Ivanović, Ana; Kalezić, Miloš L; Zelditch, Miriam L

    2014-09-01

    Comparative studies of ontogenies of closely related species provide insights into the mechanisms responsible for morphological diversification. Using geometric morphometrics, we investigated the ontogenetic dynamics of postlarval skull shape and disparity in three closely related crested newt species. The skull shapes of juveniles just after metamorphosis (hereafter metamorphs) and adult individuals were sampled by landmark configurations that describe the shape of the dorsal and ventral side of the newt skull, and analyzed separately. The three species differ in skull size and shape in metamorphs and adults. The ontogenies of dorsal and ventral skull differ in the orientation but not lengths of the ontogenetic trajectories. The disparity of dorsal skull shape increases over ontogeny, but that of ventral skull shape does not. Thus, modifications of ontogenetic trajectories can, but need not, increase the disparity of shape. In species with biphasic life-cycles, when ontogenetic trajectories for one stage can be decoupled from those of another, increases and decreases in disparity are feasible, but our results show that they need not occur. © 2014 Wiley Periodicals, Inc.

  20. European early modern humans and the fate of the Neandertals

    PubMed Central

    Trinkaus, Erik

    2007-01-01

    A consideration of the morphological aspects of the earliest modern humans in Europe (more than ≈33,000 B.P.) and the subsequent Gravettian human remains indicates that they possess an anatomical pattern congruent with the autapomorphic (derived) morphology of the earliest (Middle Paleolithic) African modern humans. However, they exhibit a variable suite of features that are either distinctive Neandertal traits and/or plesiomorphic (ancestral) aspects that had been lost among the African Middle Paleolithic modern humans. These features include aspects of neurocranial shape, basicranial external morphology, mandibular ramal and symphyseal form, dental morphology and size, and anteroposterior dental proportions, as well as aspects of the clavicles, scapulae, metacarpals, and appendicular proportions. The ubiquitous and variable presence of these morphological features in the European earlier modern human samples can only be parsimoniously explained as a product of modest levels of assimilation of Neandertals into early modern human populations as the latter dispersed across Europe. This interpretation is in agreement with current analyses of recent and past human molecular data. PMID:17452632

  1. Three-Dimensional Morphological and Chemical Evolution of Nanoporous Stainless Steel by Liquid Metal Dealloying [3D Morphological and Chemical Evolution of Nanoporous Stainless Steel by Liquid Metal Dealloying

    DOE PAGES

    Zhao, Chonghang; Wada, Takeshi; De Andrade, Vincent; ...

    2017-09-04

    Nanoporous materials, especially those fabricated by liquid metal dealloying processes, possess great potential in a wide range of applications due to their high surface area, bicontinuous structure with both open pores for transport and solid phase for conductivity or support, and low material cost. Here, we used X-ray nanotomography and X-ray fluorescence microscopy to reveal the three-dimensional (3D) morphology and elemental distribution within materials. Focusing on nanoporous stainless steel, we evaluated the 3D morphology of the dealloying front and established a quantitative processing-structure-property relationship at a later stage of dealloying. The morphological differences of samples created by liquid metal dealloyingmore » and aqueous dealloying methods were also discussed. Here, we concluded that it is particularly important to consider the dealloying, coarsening, and densification mechanisms in influencing the performance-determining, critical 3D parameters, such as tortuosity, pore size, porosity, curvature, and interfacial shape.« less

  2. Three-Dimensional Morphological and Chemical Evolution of Nanoporous Stainless Steel by Liquid Metal Dealloying [3D Morphological and Chemical Evolution of Nanoporous Stainless Steel by Liquid Metal Dealloying

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zhao, Chonghang; Wada, Takeshi; De Andrade, Vincent

    Nanoporous materials, especially those fabricated by liquid metal dealloying processes, possess great potential in a wide range of applications due to their high surface area, bicontinuous structure with both open pores for transport and solid phase for conductivity or support, and low material cost. Here, we used X-ray nanotomography and X-ray fluorescence microscopy to reveal the three-dimensional (3D) morphology and elemental distribution within materials. Focusing on nanoporous stainless steel, we evaluated the 3D morphology of the dealloying front and established a quantitative processing-structure-property relationship at a later stage of dealloying. The morphological differences of samples created by liquid metal dealloyingmore » and aqueous dealloying methods were also discussed. Here, we concluded that it is particularly important to consider the dealloying, coarsening, and densification mechanisms in influencing the performance-determining, critical 3D parameters, such as tortuosity, pore size, porosity, curvature, and interfacial shape.« less

  3. Wear Debris Characterization and Corresponding Biological Response: Artificial Hip and Knee Joints

    PubMed Central

    Nine, Md J.; Choudhury, Dipankar; Hee, Ay Ching; Mootanah, Rajshree; Osman, Noor Azuan Abu

    2014-01-01

    Wear debris, of deferent sizes, shapes and quantities, generated in artificial hip and knees is largely confined to the bone and joint interface. This debris interacts with periprosthetic tissue and may cause aseptic loosening. The purpose of this review is to summarize and collate findings of the recent demonstrations on debris characterization and their biological response that influences the occurrence in implant migration. A systematic review of peer-reviewed literature is performed, based on inclusion and exclusion criteria addressing mainly debris isolation, characterization, and biologic responses. Results show that debris characterization largely depends on their appropriate and accurate isolation protocol. The particles are found to be non-uniform in size and non-homogeneously distributed into the periprosthetic tissues. In addition, the sizes, shapes, and volumes of the particles are influenced by the types of joints, bearing geometry, material combination, and lubricant. Phagocytosis of wear debris is size dependent; high doses of submicron-sized particles induce significant level of secretion of bone resorbing factors. However, articles on wear debris from engineered surfaces (patterned and coated) are lacking. The findings suggest considering debris morphology as an important parameter to evaluate joint simulator and newly developed implant materials. PMID:28788496

  4. Three-dimensional morphology of first molars in relation to ethnicity and the occurrence of cleft lip and palate.

    PubMed

    Echtermeyer, Sandra; Metelmann, Philine H; Hemprich, Alexander; Dannhauer, Karl-Heinz; Krey, Karl-Friedrich

    2017-01-01

    This study aims to describe morphological peculiarities of maxillary and mandibular first molars in Europeans, Asians and Europeans with cleft lip and palate. Reflex microscopy was used to obtain three-dimensional morphometric landmarks from 40 models (11 Europeans and 13 Asians without cleft lip and palate, 16 Europeans with unilateral cleft lip and palate). The cases were examined using traditional morphometry and geometric morphometry, and visualized using thin-plate splines. Classic morphometry showed no right/left differences in the study groups and no significant differences with regard to the cleft side in patients with cleft lip and palate. In Asians, a significantly greater mesiodistal width was found. Geometric morphometry showed an enlarged centroid size in Asians (maxilla and mandible). In cleft patients, the cleft site did not appear to impact the morphology of first molars. Unilateral clefting did not affect the size and shape of molars; however, characteristic ethnicity-based differences were in fact identified. The results are relevant for orthodontic treatment with preadjusted appliances, and prosthetic CAD/CAM restorations.

  5. Morphological and molecular characterization of Paramecium (Viridoparamecium nov. subgen.) chlorelligerum Kahl (Ciliophora).

    PubMed

    Kreutz, Martin; Stoeck, Thorsten; Foissner, Wilhelm

    2012-01-01

    We redescribe Paramecium chlorelligerum, a forgotten species, which Kahl (Tierwelt Dtl., 1935, 30:651) briefly but precisely described in the addendum to his ciliate monographs as a Paramecium with symbiotic green algae. The redescription is based on classical morphological methods and the analysis of the small subunit (SSU) rDNA. Morphologically, P. chlorelligerum differs from P. (C.) bursaria, the second green species in the genus, by having a special swimming shape, the length of the caudal cilia, the size of the micronucleus, the size of the symbiotic algae, the contractile vacuoles (with collecting vesicles vs. collecting canals), and the number of excretory pores/contractile vacuole (1 vs. 2-3). The molecular investigations show that P. chlorelligerum forms a distinct branch distant from the P. (Chloroparamecium) bursaria clade. Thus, we classify P. chlorelligerum in a new subgenus: Paramecium (Viridoparamecium) chlorelligerum. The symbiotic alga belongs to the little-known genus Meyerella, as yet recorded only from the plankton of a North American lake. © 2012 The Author(s) Journal of Eukaryotic Microbiology © 2012 International Society of Protistologists.

  6. Effect of Prior Austenite Grain Size on the Morphology of Nano-Bainitic Steels

    NASA Astrophysics Data System (ADS)

    Singh, Kritika; Kumar, Avanish; Singh, Aparna

    2018-04-01

    The strength in nanostructured bainitic steels primarily arises from the fine platelets of bainitic ferrite embedded in carbon-enriched austenite. However, the toughness is dictated by the shape and volume fraction of the retained austenite. Therefore, the exact determination of processing-morphology relationships is necessary to design stronger and tougher bainite. In the current study, the morphology of bainitic ferrite in Fe-0.89C-1.59Si-1.65Mn-0.37Mo-1Co-0.56Al-0.19Cr (wt pct) bainitic steel has been investigated as a function of the prior austenite grain size (AGS). Specimens were austenitized at different temperatures ranging from 900 °C to 1150 °C followed by isothermal transformation at 300 °C. Detailed microstructural characterization has been carried out using scanning electron microscopy and X-ray diffraction. The results showed that the bainitic laths transformed in coarse austenite grains are finer resulting in higher hardness, whereas smaller austenite grains lead to the formation of thicker bainitic laths with a large fraction of blocky type retained austenite resulting in lower hardness.

  7. Effect of inductively coupled plasma surface treatment on silica gel and mesoporous MCM-41 particles

    NASA Astrophysics Data System (ADS)

    J, A. JUAREZ-MORENO; U, CHACON-ARGAEZ; J, BARRON-ZAMBRANO; C, CARRERA-FIGUEIRAS; P, QUINTANA-OWEN; W, TALAVERA-PECH; Y, PEREZ-PADILLA; A, AVILA-ORTEGA

    2018-06-01

    Silica gel and MCM-41 synthesized mesoporous materials were treated with either oxygen (O2), hexamethyldisiloxane (HMDSO) and organic vapors like ethanol (EtOH), and acrylonitrile (AN) inductive plasma. The radiofrequency power for the modification was fixed to 120 W and 30 min, assuring a high degree of organic ionization energy in the plasma. The surface properties were studied by infrared spectroscopy (FTIR), scanning electron microscopy, x-ray photoelectron spectroscopy and dynamic light scattering technique was used for characterizing size distributions. When the silica and MCM-41 particles were modified by AN and HMDSO plasma gases, the surface morphology of the particles was changed, presenting another color, size or shape. In contrast, the treatments of oxygen and EtOH did not affect the surface morphology of both particles, but increased the oxygen content at the surface bigger than the AN and HMDSO plasma treatments. In this study, we investigated the influence of different plasma treatments on changes in morphology and the chemical composition of the modified particles which render them a possible new adsorbent for utilization in sorptive extraction techniques for polar compounds.

  8. Beak and skull shapes of human commensal and non-commensal house sparrows Passer domesticus

    PubMed Central

    2013-01-01

    Background The granivorous house sparrow Passer domesticus is thought to have developed its commensal relationship with humans with the rise of agriculture in the Middle East some 10,000 years ago, and to have expanded with the spread of agriculture in Eurasia during the last few thousand years. One subspecies, P. d. bactrianus, residing in Central Asia, has apparently maintained the ancestral ecology, however. This subspecies is not associated with human settlements; it is migratory and lives in natural grass- and wetland habitats feeding on wild grass seeds. It is well documented that the agricultural revolution was associated with an increase in grain size and changes in seed structure in cultivated cereals, the preferred food source of commensal house sparrow. Accordingly, we hypothesize that correlated changes may have occurred in beak and skull morphology as adaptive responses to the change in diet. Here, we test this hypothesis by comparing the skull shapes of 101 house sparrows from Iran, belonging to five different subspecies, including the non-commensal P. d. bactrianus, using geometric morphometrics. Results The various commensal house sparrow subspecies share subtle but consistent skeletal features that differ significantly from those of the non-commensal P. d. bactrianus. Although there is a marked overall size allometry in the data set, the shape difference between the ecologically differentiated sparrows cannot be explained by differences in size alone. Relative to the size allometry commensal house sparrows exhibit a skull shape consistent with accelerated development (heterochrony), resulting in a more robust facial cranium and a larger, more pointed beak. Conclusion The difference in skull shape and robustness of the beak between commensal and non-commensal house sparrows is consistent with adaptations to process the larger and rachis encapsulated seeds of domesticated cereals among human associated populations. PMID:24044497

  9. Ontogeny of head and caudal fin shape of an apex marine predator: The tiger shark (Galeocerdo cuvier).

    PubMed

    Fu, Amy L; Hammerschlag, Neil; Lauder, George V; Wilga, Cheryl D; Kuo, Chi-Yun; Irschick, Duncan J

    2016-05-01

    How morphology changes with size can have profound effects on the life history and ecology of an animal. For apex predators that can impact higher level ecosystem processes, such changes may have consequences for other species. Tiger sharks (Galeocerdo cuvier) are an apex predator in tropical seas, and, as adults, are highly migratory. However, little is known about ontogenetic changes in their body form, especially in relation to two aspects of shape that influence locomotion (caudal fin) and feeding (head shape). We captured digital images of the heads and caudal fins of live tiger sharks from Southern Florida and the Bahamas ranging in body size (hence age), and quantified shape of each using elliptical Fourier analysis. This revealed changes in the shape of the head and caudal fin of tiger sharks across ontogeny. Smaller juvenile tiger sharks show an asymmetrical tail with the dorsal (upper) lobe being substantially larger than the ventral (lower) lobe, and transition to more symmetrical tail in larger adults, although the upper lobe remains relatively larger in adults. The heads of juvenile tiger sharks are more conical, which transition to relatively broader heads over ontogeny. We interpret these changes as a result of two ecological transitions. First, adult tiger sharks can undertake extensive migrations and a more symmetrical tail could be more efficient for swimming longer distances, although we did not test this possibility. Second, adult tiger sharks expand their diet to consume larger and more diverse prey with age (turtles, mammals, and elasmobranchs), which requires substantially greater bite area and force to process. In contrast, juvenile tiger sharks consume smaller prey, such as fishes, crustaceans, and invertebrates. Our data reveal significant morphological shifts in an apex predator, which could have effects for other species that tiger sharks consume and interact with. © 2016 Wiley Periodicals, Inc.

  10. Bythaelurus bachi n. sp., a new deep-water catshark (Carcharhiniformes, Scyliorhinidae) from the southwestern Indian Ocean, with a review of Bythaelurus species and a key to their identification.

    PubMed

    Weigmann, Simon; Ebert, David A; Clerkin, Paul J; Stehmann, Matthias F W; Naylor, Gavin J P

    2016-12-19

    A new deep-water catshark, Bythaelurus bachi, is described based on 44 specimens caught on the southern Madagascar Ridge in the southwestern Indian Ocean. The new species is the only stout-bodied Bythaelurus with oral papillae in the region and is distinguished from all congeners by the plain beige to light gray-brown coloration, high diversity in dermal denticle morphology, and presence of composite oral papillae. Despite resemblance in body shape, Bythaelurus bachi n. sp. is distinguished from its closest congener, B. naylori Ebert & Clerkin, 2015, by the presence of numerous large, partially composite papillae on the tongue and roof of the mouth (vs. papillae lacking), plain light coloration (vs. medium to dark brown ground color, light fin edges and a distinctly dark dusky-colored snout), only slightly enlarged dermal denticles on the anterior upper caudal-fin margin (vs. dermal denticles distinctly enlarged), a higher diversity in dermal denticle morphology in general, and smaller maximum size and size at maturity. The distinction of both species is also supported by molecular results. The new species differs from all other congeners in the western Indian Ocean in the stout body shape of large specimens, coloration, larger size, as well as several morphometrics, including larger claspers, longer eyes and dorsal fins, and shorter pelvic-anal and pelvic-caudal spaces. The genus is reviewed, a key to its species given.

  11. Shaping electrocatalysis through tailored nanomaterials

    DOE PAGES

    Kang, Yijin; Yang, Peidong; Markovic, Nenad M.; ...

    2016-09-21

    Electrocatalysis is a subclass of heterogeneous catalysis that is aimed towards increase of the electrochemical reaction rates that are taking place at the surface of electrodes. Real-world electrocatalysts are usually based on precious metals in the form of nanoparticles due to their high surface-to-volume ratio, which enables better utilization of employed materials. Ability to tailor nanostructure of an electrocatalyst is critical in order to tune their electrocatalytic properties. Over the last decade, that has mainly been achieved through implementation of fundamental studies performed on well-defined extended surfaces with distinct single crystalline and polycrystalline structures. Based on these studies, it hasmore » been demonstrated that performance of an electrocatalyst could be significantly changed through the control of size, composition, morphology and architecture of employed nanomaterials. Here, this review outlines the following steps in the process of rational development of an efficient electrocatalyst: 1) electrochemical properties of well-defined surfaces, 2) synthesis and characterization of different classes of electrocatalysts, and 3) correlation between physical properties (size, shape, composition and morphology) and electrochemical behavior (adsorption, electrocatalytic activity and durability) of electrocatalyst. In addition, this is a brief summary of the novel research platforms in the development of functional nano materials for energy conversion and storage applications such as fuel cells electrolyzers and batteries.« less

  12. Cuprous Oxide Scale up: Gram Production via Bulk Synthesis using Classic Solvents at Low Temperatures

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hall, A.; Han, T. Y.

    Cuprous oxide is a p-type semiconducting material that has been highly researched for its interesting properties. Many small-scale syntheses have exhibited excellent control over size and morphology. As the demand for cuprous oxide grows, the synthesis method need to evolve to facilitate large-scale production. This paper supplies a facile bulk synthesis method for Cu₂O on average, 1-liter reaction volume can produce 1 gram of particles. In order to study the shape and size control mechanisms on such a scale, the reaction volume was diminished to 250 mL producing on average 0.3 grams of nanoparticles per batch. Well-shaped nanoparticles have beenmore » synthesized using an aqueous solution of CuCl₂, NaOH, SDS surfactant, and NH₂OH-HCl at mild temperatures. The time allotted between the addition of NaOH and NH₂OH-HCl was determined to be critical for Cu(OH)2 production, an important precursor to the final produce The effects of stirring rates on a large scale was also analyzed during reagent addition and post reagent addition. A morphological change from rhombic dodecahedra to spheres occurred as the stirring speed was increased. The effects of NH₂OH-HCl concentration were also studied to control the etching effects of the final product.« less

  13. Morphological and mechanical analyses of laminates manufactured from randomly positioned carbon fibre/epoxy resin prepreg scraps

    NASA Astrophysics Data System (ADS)

    Souza, Christiane S. R.; Cândido, Geraldo M.; Alves, Wellington; Marlet, José Maria F.; Rezende, Mirabel C.

    2017-10-01

    This study aims to contribute to sustainability by proposing the reuse of composite prepreg scrap as an added value from discards. The research evaluates the microstructure and mechanical properties of laminates processed by the reuse of uncured carbon fibre/F155-epoxy resin prepreg scraps, waste from the ply cutting area of an aeronautical industry. The composite scraps were used as collected and were randomly positioned to produce laminates to be cured at an autoclave. The mechanical characterization shows a decrease of 39% for the compression property due to the discontinuous fibres in the laminate and an increase of 34% for the interlaminar shear strength, when compared to continuous fibre laminates. This increase is attributed to the higher crosslink density of the epoxy resin, as a result of the cure temperature used in autoclave (60 °C higher than suggested by supplier) and also to the randomly positioned scraps. Microscopic analyses confirm the consolidation of laminates, although show resin rich areas with different sizes and shapes attributed to the overlapping of the scraps with different sizes and shapes. These resin rich areas may contribute to decrease the mechanical properties of laminates. The correlation between mechanical and morphological results shows potential to be used on non-critical structural application, as composite jigs, contributing to sustainability.

  14. Is the area of the orbital opening in humans related to climate?

    PubMed

    Tomaszewska, Agnieszka; Kwiatkowska, Barbara; Jankauskas, Rimantas

    2015-01-01

    The aim of this study was to evaluate whether climatic conditions impact the size of the anterior orbital opening in humans. The previous research has shown that morphology of the human orbit, a trait strongly related to the shape of the cranium, varies significantly among populations. However, the mechanisms of this variation are still debatable. Besides such evolutionary forces as genetic drift, climatic conditions may be involved. Thermoregulatory processes affect skull shape, and thus may also influence orbital morphology. A total of 846 dry skulls of male and female adults from three climatic areas (i.e., warm, temperate, and cold) of Europe were evaluated. The areas of the left and right orbital openings were measured using the three-dimensional contact scanner MicroScribe G2L, and analyzed with regard to climate. The results reveal a statistically significant association with climatic conditions on the area of orbital opening in accordance with Bergmann's rule. The anterior orbital opening area was smaller in male individuals from the cold climate, and larger in individuals from the warm climate areas. These data may support the hypothesized association between size of the orbital opening and adaptation to different climatic conditions, but only in males. © 2015 Wiley Periodicals, Inc.

  15. Morphology of blazar-induced gamma ray halos due to a helical intergalactic magnetic field

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Long, Andrew J.; Vachaspati, Tanmay, E-mail: andrewjlong@asu.edu, E-mail: tvachasp@asu.edu

    We study the characteristic size and shape of idealized blazar-induced cascade halos in the 1–100,GeV energy range assuming various non-helical and helical configurations for the intergalactic magnetic field (IGMF). While the magnetic field creates an extended halo, the helicity provides the halo with a twist. Under simplifying assumptions, we assess the parameter regimes for which it is possible to measure the size and shape of the halo from a single source and then to deduce properties of the IGMF. We find that blazar halo measurements with an experiment similar to Fermi-LAT are best suited to probe a helical magnetic fieldmore » with strength and coherence length today in the ranges 10{sup −17} ∼< B{sub 0} / Gauss ∼< 10{sup −13} and 10 Mpc ∼< λ ∼< 10 Gpc where H ∼ B{sub 0}{sup 2} / λ is the magnetic helicity density. Stronger magnetic fields or smaller coherence scales can still potentially be investigated, but the connection between the halo morphology and the magnetic field properties is more involved. Weaker magnetic fields or longer coherence scales require high photon statistics or superior angular resolution.« less

  16. The genus Krithe (Ostracoda) from the Campanian and Maastrichtian (Upper Cretaceous) of the northern US Gulf Coastal Plain

    USGS Publications Warehouse

    Puckett, T.M.

    1997-01-01

    The ostracode genus Krithe is one of the most common genera in the Upper Cretaceous (late Santonian to Maastrichtian) deposits of the northern Gulf Coastal Plain of North America. Although it is never abundant, the genus occurs in sediments that were deposited under a wide range of palaeoenvironments, including nearshore sandy marls to offshore, nearly pure, chalk. The taxonomy of this taxon has been problematical, and what is herein considered to be a single species, K. cushmani, has been referred to in the literature under five different names. Two morphotypes were observed: relatively large individuals with 'mushroom'-shaped vestibules collected from chalk, and smaller individuals with pocket-shaped vestibules collected from nearshore deposits. Species of Krithe have been hypothesized to be useful in estimating dissolved oxygen concentration in ancient ocean floors, based on details of their morphology. Whereas the relationship between size and environment corroborates with previous predictions (larger individuals live in deeper water), the morphology of the vestibules contradicts predictions (the larger vestibules occur in the nearshore deposits and the smaller, more constricted vestibules occur in the chalk). A causal relationship between environment and morphology is discussed.

  17. Morphometric assessment of pterosaur jaw disparity

    PubMed Central

    Navarro, Charlie A.; Martin-Silverstone, Elizabeth

    2018-01-01

    Pterosaurs were a successful group of Mesozoic flying reptiles. They were the first vertebrate group to achieve powered flight and varied enormously in morphology and ecology, occupying a variety of niches and developing specialized feeding strategies. Ecomorphological principles suggest this variation should be reflected by great morphological diversity in the lower jaw, given that the mandible served as the primary apparatus for prey acquisition. Here we present the first study of mandibular shape disparity in pterosaurs and aim to characterize major aspects of variation. We use a combination of geometric morphometric approaches, incorporating both outline analysis using elliptical Fourier analysis and semi-landmark approaches. Our results show that morphological convergence is prevalent and many pterosaurs, belonging to diverse dietary groups and subclades, overlap in morphospace and possessed relatively simple ‘rod-shaped’ jaws. There is no clear trend of size distributions in pterosaur mandibular morphospace, and larger forms are widely distributed. Additionally, there is limited functional signal within pterosaur lower jaw morphospace. Instead, the development of a large anterior ventral crest represents the major component of disparity. This suggests that a socio-sexual trait was a key driver for innovation in pterosaur lower jaw shape. PMID:29765665

  18. Determination of channel-morphology characteristics, bankfull discharge, and various design-peak discharges in western Montana

    USGS Publications Warehouse

    Lawlor, Sean M.

    2004-01-01

    Stream-restoration projects using natural stream designs typically are based on channel configurations that can accommodate a wide range of streamflow and sediment-transport conditions without excessive erosion or deposition. Bankfull discharge is an index of streamflow considered to be closely related to channel shape, size, and slope (channel morphology). Because of the need for more information about the relation between channel morphology and bankfull discharge, the U.S. Geological Survey (USGS), in cooperation with the Montana Department of Transportation and the U.S. Department of Agriculture-Lolo National Forest, conducted a study to collect channel-morphology and bankfull-discharge data at gaged sites and use these data to improve current (2004) methods of estimation of bankfull discharge and various design-peak discharges at ungaged sites. This report presents channel-morphology characteristics, bankfull discharge, and various design-peak discharges for 41 sites in western Montana. Channel shape, size, and slope and bankfull discharge were determined at 41 active or discontinued USGS streamflow-gaging sites in western Montana. The recurrence interval for the bankfull discharge for this study ranged from 1.0 to 4.4 years with a median value of 1.5 years. The relations between channel-morphology characteristics and various design-peak discharges were examined using regression analysis. The analyses showed that the only characteristics that were significant for all peak discharges were either bankfull width or bankfull cross-sectional area. Bankfull discharge at ungaged sites in most of the study area can be estimated by application of a multiplier after determining the 2-year peak discharge at the ungaged site. The multiplier, which is the ratio of bankfull discharge to the 2-year peak discharge determined at the 41 sites, ranged from 0.21 to 3.7 with a median value of 0.84. Regression relations between bankfull discharge and drainage area and between bankfull width and drainage area were examined for three ranges of mean annual precipitation. The results of the regression analyses indicated that both drainage area and mean annual precipitation were significantly related (p values less than 0.05) to bankfull discharge.

  19. Source Identification Of Airborne Antimony On The Basis Of The Field Monitoring And The Source Profiling

    NASA Astrophysics Data System (ADS)

    Iijima, A.; Sato, K.; Fujitani, Y.; Fujimori, E.; Tanabe, K.; Ohara, T.; Shimoda, M.; Kozawa, K.; Furuta, N.

    2008-12-01

    The results of the long-term monitoring of airborne particulate matter (APM) in Tokyo indicated that APM have been extremely enriched with antimony (Sb) compared to crustal composition. This observation suggests that the airborne Sb is distinctly derived from human activities. According to the material flow analysis, automotive brake abrasion dust and fly ash from waste incinerator were suspected as the significant Sb sources. To clarify the emission sources of the airborne Sb, elemental composition, particle size distribution, and morphological profiles of dust particles collected from two possible emission sources were characterized and compared to the field observation data. Brake abrasion dust samples were generated by using a brake dynamometer. During the abrasion test, particle size distribution was measured by an aerodynamic particle sizer spectrometer. Concurrently, size- classified dust particles were collected by an Andersen type air sampler. Fly ash samples were collected from several municipal waste incinerators, and the bulk ash samples were re-dispersed into an enclosed chamber. The measurement of particle size distribution and the collection of size-classified ash particles were conducted by the same methodologies as described previously. Field observations of APM were performed at a roadside site and a residential site by using an Andersen type air sampler. Chemical analyses of metallic elements were performed by an inductively coupled plasma atomic emission spectrometry and an inductively coupled plasma mass spectrometr. Morphological profiling of the individual particle was conducted by a scanning electron microscope equipped with an energy dispersive X-ray spectrometer. High concentration of Sb was detected from both of two possible sources. Particularly, Sb concentrations in a brake abrasion dust were extremely high compared to that in an ambient APM, suggesting that airborne Sb observed at the roadside might have been largely derived from mechanical abrasion of automotive brake pads. The peak of the mass-based particle size distribution of brake abrasion dust was found in a diameter of 2-3 μm. From the morphological viewpoints, shape of brake abrasion dust particle was typically edge- shaped, and high concentrated Sb and sulfur were simultaneously detected in a brake abrasion dust particle because Sb2S3 is used as a solid lubricant for automotive brake pad. Indeed, at the roadside site, total concentration of airborne Sb was twice as much as that observed at residential site. Moreover, the most concentrated Sb was found in a diameter of 2.1-3.6 μm for the roadside APM. Furthermore, in the collected particles with this size range, we found a number of particles of which morphological profiles were similar to those of the brake abrasion dust. Consequently, an automotive brake abrasion dust is expected as the predominant source of airborne Sb in the roadside atmosphere.

  20. Men's facial masculinity: when (body) size matters.

    PubMed

    Holzleitner, Iris J; Hunter, David W; Tiddeman, Bernard P; Seck, Alassane; Re, Daniel E; Perrett, David I

    2014-01-01

    Recent studies suggest that judgments of facial masculinity reflect more than sexually dimorphic shape. Here, we investigated whether the perception of masculinity is influenced by facial cues to body height and weight. We used the average differences in three-dimensional face shape of forty men and forty women to compute a morphological masculinity score, and derived analogous measures for facial correlates of height and weight based on the average face shape of short and tall, and light and heavy men. We found that facial cues to body height and weight had substantial and independent effects on the perception of masculinity. Our findings suggest that men are perceived as more masculine if they appear taller and heavier, independent of how much their face shape differs from women's. We describe a simple method to quantify how body traits are reflected in the face and to define the physical basis of psychological attributions.

  1. Reduction-responsive interlayer-crosslinked micelles prepared from star-shaped copolymer via click chemistry for drug controlled release

    NASA Astrophysics Data System (ADS)

    Dai, Yu; Wang, Hongquan; Zhang, Xiaojin

    2017-12-01

    To improve the stability of polymeric micelles, here we describe interlayer-crosslinked micelles prepared from star-shaped copolymer via click chemistry. The formation of interlayer-crosslinked micelles was investigated and confirmed by proton nuclear magnetic resonance, Fourier-transform infrared spectroscopy, and fluorescence spectroscopy. The morphology of un-crosslinked micelles and crosslinked micelles observed by transmission electron microscope is both uniform nano-sized spheres (approximately 20 nm). The crosslinking enhances the stability of polymeric micelles and improves the drug loading capacity of polymeric micelles. The interlayer-crosslinked micelles prepared from star-shaped copolymer and a crosslinker containing a disulfide bond are reduction-responsive and can release the drug quickly in the presence of the reducing agents such as glutathione (GSH).

  2. Morphological and XPS study of ball milled Fe1-xAlx (0.3≤x≤0.6) alloys

    NASA Astrophysics Data System (ADS)

    Rajan, Sandeep; Kumar, Anil; Vyas, Anupam; Brajpuriya, Ranjeet

    2018-05-01

    The paper presents mechanical and XPS study of ball milled Fe1-xAlx (0.3≤x≤0.6) alloys. The author prepared the solid solution of Fe(Al) with different composition of Al by using mechanical alloying (MA) technique. The MA process induces a progressive dissolution of Al into Fe, resulted in the formation of an extended Fe(Al) solid solution with the bcc structure after 5 hr of milling. The SEM Images shows that the initial shape of particles disappeared completely, and their structure became a mixture of small and large angular-shaped crystallites with different sizes. The TEM micrograph also confirms the reduction in crystallite size and alloy formation. XPS study shows the shift in the binding energy position of both Fe and Al Peaks provide strong evidence of Fe(Al) phase formation after milling.

  3. Tuning the anticancer activity of a novel pro-apoptotic peptide using gold nanoparticle platforms

    PubMed Central

    Akrami, Mohammad; Balalaie, Saeed; Hosseinkhani, Saman; Alipour, Mohsen; Salehi, Fahimeh; Bahador, Abbas; Haririan, Ismaeil

    2016-01-01

    Pro-apoptotic peptides induce intrinsic apoptosis pathway in cancer cells. However, poor cellular penetration of the peptides is often associated with limited therapeutic efficacy. In this report, a series of peptide-gold nanoparticle platforms were developed to evaluate the anticancer activity of a novel alpha-lipoic acid-peptide conjugate, LA-WKRAKLAK, with respect to size and shape of nanoparticles. Gold nanoparticles (AuNPs) were found to enhance cell internalization as well as anticancer activity of the peptide conjugates. The smaller nanospheres showed a higher cytotoxicity, morphological change and cellular uptake compared to larger nanospheres and nanorods, whereas nanorods showed more hemolytic activity compared to nanospheres. The findings suggested that the anticancer and biological effects of the peptides induced by intrinsic apoptotic pathway were tuned by peptide-functionalized gold nanoparticles (P-AuNPs) as a function of their size and shape. PMID:27491007

  4. Temperature dependent localized surface plasmon resonance properties of supported gold nanoparticles

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Laha, Ranjit; Ranjan, Pranay

    2016-05-23

    The well known localized surface plasmon resonance (LSPR) of gold nanoparticles (AuNPs) supported on a dielectric substrate depends on the particle shape, size and type of dielectric material. The particle size and shape mainly vary with the method of preparation and the parameters involved there in. In this report, we show preparation of AuNPs supported on quartz substrate by direct current sputtering followed by thermal annealing at an optimized temperature of 400 °C. The samples were characterized using optical absorption spectra, scanning electron microscopy (SEM) and the energy dispersive x-ray spectrum. The LSPR position could be tuned by varying annealingmore » temperature. The LSPR was found to be blue shifted up to 10 nm with annealing temperature varying from 400 °C to 800 °C. The change in LSPR was ascribed to the morphology of AuNPs over quartz.« less

  5. Tuning the anticancer activity of a novel pro-apoptotic peptide using gold nanoparticle platforms

    NASA Astrophysics Data System (ADS)

    Akrami, Mohammad; Balalaie, Saeed; Hosseinkhani, Saman; Alipour, Mohsen; Salehi, Fahimeh; Bahador, Abbas; Haririan, Ismaeil

    2016-08-01

    Pro-apoptotic peptides induce intrinsic apoptosis pathway in cancer cells. However, poor cellular penetration of the peptides is often associated with limited therapeutic efficacy. In this report, a series of peptide-gold nanoparticle platforms were developed to evaluate the anticancer activity of a novel alpha-lipoic acid-peptide conjugate, LA-WKRAKLAK, with respect to size and shape of nanoparticles. Gold nanoparticles (AuNPs) were found to enhance cell internalization as well as anticancer activity of the peptide conjugates. The smaller nanospheres showed a higher cytotoxicity, morphological change and cellular uptake compared to larger nanospheres and nanorods, whereas nanorods showed more hemolytic activity compared to nanospheres. The findings suggested that the anticancer and biological effects of the peptides induced by intrinsic apoptotic pathway were tuned by peptide-functionalized gold nanoparticles (P-AuNPs) as a function of their size and shape.

  6. Nanoparticle characterization by means of scanning free grazing emission X-ray fluorescence

    NASA Astrophysics Data System (ADS)

    Kayser, Yves; Sá, Jacinto; Szlachetko, Jakub

    2015-05-01

    Nanoparticles are considered for applications in domains as various as medical and pharmaceutical sciences, opto- and microelectronics, catalysis, photovoltaics, spintronics or nano- and biotechnology. The applications realized with nanocrystals depend strongly on the physical dimensions (shape and size) and elemental constitution. We demonstrate here that grazing emission X-ray fluorescence (GEXRF) is an element sensitive technique that presents the potential for a reliable and accurate determination of the morphology of nanoparticles deposited on a flat substrate (ready-to-use devices). Thanks to the scanning-free approach of the used GEXRF setup, the composition, shape and average size of nanoparticles are determined in short time intervals, minimizing the exposure to radiation. The (scanning-free) GEXRF technique allows for in situ investigations of the nanoparticulate systems thanks to the penetration properties of both the probe X-ray beam and the emitted X-ray fluorescence signal.

  7. Temperature dependent localized surface plasmon resonance properties of supported gold nanoparticles

    NASA Astrophysics Data System (ADS)

    Laha, Ranjit; Ranjan, Pranay

    2016-05-01

    The well known localized surface plasmon resonance (LSPR) of gold nanoparticles (AuNPs) supported on a dielectric substrate depends on the particle shape, size and type of dielectric material. The particle size and shape mainly vary with the method of preparation and the parameters involved there in. In this report, we show preparation of AuNPs supported on quartz substrate by direct current sputtering followed by thermal annealing at an optimized temperature of 400 °C. The samples were characterized using optical absorption spectra, scanning electron microscopy (SEM) and the energy dispersive x-ray spectrum. The LSPR position could be tuned by varying annealing temperature. The LSPR was found to be blue shifted up to 10 nm with annealing temperature varying from 400 °C to 800 °C. The change in LSPR was ascribed to the morphology of AuNPs over quartz.

  8. Size- and Shape-Dependent Antibacterial Studies of Silver Nanoparticles Synthesized by Wet Chemical Routes

    PubMed Central

    Raza, Muhammad Akram; Kanwal, Zakia; Rauf, Anum; Sabri, Anjum Nasim; Riaz, Saira; Naseem, Shahzad

    2016-01-01

    Silver nanoparticles (AgNPs) of different shapes and sizes were prepared by solution-based chemical reduction routes. Silver nitrate was used as a precursor, tri-sodium citrate (TSC) and sodium borohydride as reducing agents, while polyvinylpyrrolidone (PVP) was used as a stabilizing agent. The morphology, size, and structural properties of obtained nanoparticles were characterized by scanning electron microscopy (SEM), UV-visible spectroscopy (UV-VIS), and X-ray diffraction (XRD) techniques. Spherical AgNPs, as depicted by SEM, were found to have diameters in the range of 15 to 90 nm while lengths of the edges of the triangular particles were about 150 nm. The characteristic surface plasmon resonance (SPR) peaks of different spherical silver colloids occurring in the wavelength range of 397 to 504 nm, whereas triangular particles showed two peaks, first at 392 nm and second at 789 nm as measured by UV-VIS. The XRD spectra of the prepared samples indicated the face-centered cubic crystalline structure of metallic AgNPs. The in vitro antibacterial properties of all synthesized AgNPs against two types of Gram-negative bacteria, Pseudomonas aeruginosa and Escherichia coli were examined by Kirby–Bauer disk diffusion susceptibility method. It was noticed that the smallest-sized spherical AgNPs demonstrated a better antibacterial activity against both bacterial strains as compared to the triangular and larger spherical shaped AgNPs. PMID:28335201

  9. Electrochemical synthesis and characterization of zinc oxalate nanoparticles

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shamsipur, Mojtaba, E-mail: mshamsipur@yahoo.com; Roushani, Mahmoud; Department of Chemistry, Ilam University, Ilam

    2013-03-15

    Highlights: ► Synthesis of zinc oxalate nanoparticles via electrolysis of a zinc plate anode in sodium oxalate solutions. ► Design of a Taguchi orthogonal array to identify the optimal experimental conditions. ► Controlling the size and shape of particles via applied voltage and oxalate concentration. ► Characterization of zinc oxalate nanoparticles by SEM, UV–vis, FT-IR and TG–DTA. - Abstract: A rapid, clean and simple electrodeposition method was designed for the synthesis of zinc oxalate nanoparticles. Zinc oxalate nanoparticles in different size and shapes were electrodeposited by electrolysis of a zinc plate anode in sodium oxalate aqueous solutions. It was foundmore » that the size and shape of the product could be tuned by electrolysis voltage, oxalate ion concentration, and stirring rate of electrolyte solution. A Taguchi orthogonal array design was designed to identify the optimal experimental conditions. The morphological characterization of the product was carried out by scanning electron microscopy. UV–vis and FT-IR spectroscopies were also used to characterize the electrodeposited nanoparticles. The TG–DTA studies of the nanoparticles indicated that the main thermal degradation occurs in two steps over a temperature range of 350–430 °C. In contrast to the existing methods, the present study describes a process which can be easily scaled up for the production of nano-sized zinc oxalate powder.« less

  10. Nanoscale Morphology of PTB7 Based Organic Photovoltaics as a Function of Fullerene Size

    DOE PAGES

    Roehling, John D.; Baran, Derya; Sit, Joseph; ...

    2016-08-08

    High efficiency polymer:fullerene photovoltaic device layers self-assemble with hierarchical features from ångströms to 100’s of nanometers. The feature size, shape, composition, orientation, and order all contribute to device efficiency and are simultaneously difficult to study due to poor contrast between carbon based materials. This study seeks to increase device efficiency and simplify morphology measurements by replacing the typical fullerene acceptor with endohedral fullerene Lu 3N@PC 80BEH. The metal atoms give excellent scattering contrast for electron beam and x-ray experiments. Additionally, Lu 3N@PC 80BEH has a lower electron affinity than standard fullerenes, which can raise the open circuit voltage of photovoltaicmore » devices. Electron microscopy techniques are used to produce a detailed account of morphology evolution in mixtures of Lu 3N@PC 80BEH with the record breaking donor polymer, PTB7 and coated using solvent mixtures. We demonstrate that common solvent additives like 1,8-diiodooctane or chloronapthalene do not improve the morphology of endohedral fullerene devices as expected. The poor device performance is attributed to the lack of mutual miscibility between this particular polymer:fullerene combination and to co-crystallization of Lu 3N@PC 80BEH with 1,8-diiodooctane. This negative result explains why solvent additives mixtures are not necessarily a morphology cure-all.« less

  11. Nanoscale Morphology of PTB7 Based Organic Photovoltaics as a Function of Fullerene Size

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Roehling, John D.; Baran, Derya; Sit, Joseph

    High efficiency polymer:fullerene photovoltaic device layers self-assemble with hierarchical features from ångströms to 100’s of nanometers. The feature size, shape, composition, orientation, and order all contribute to device efficiency and are simultaneously difficult to study due to poor contrast between carbon based materials. This study seeks to increase device efficiency and simplify morphology measurements by replacing the typical fullerene acceptor with endohedral fullerene Lu 3N@PC 80BEH. The metal atoms give excellent scattering contrast for electron beam and x-ray experiments. Additionally, Lu 3N@PC 80BEH has a lower electron affinity than standard fullerenes, which can raise the open circuit voltage of photovoltaicmore » devices. Electron microscopy techniques are used to produce a detailed account of morphology evolution in mixtures of Lu 3N@PC 80BEH with the record breaking donor polymer, PTB7 and coated using solvent mixtures. We demonstrate that common solvent additives like 1,8-diiodooctane or chloronapthalene do not improve the morphology of endohedral fullerene devices as expected. The poor device performance is attributed to the lack of mutual miscibility between this particular polymer:fullerene combination and to co-crystallization of Lu 3N@PC 80BEH with 1,8-diiodooctane. This negative result explains why solvent additives mixtures are not necessarily a morphology cure-all.« less

  12. Effect of Zirconium Addition on Microstructure and Mechanical Property of TiC/Ti6A14V Composites

    NASA Astrophysics Data System (ADS)

    Ma, Xuliang; Wang, Xiang; Li, Li; Gai, Pengtao; Zhu, Chengwu

    TiC/Ti6A14V composites with different Zr additions were prepared successfully in a consumable vacuum arc furnace equipped with a water-cooled copper crucible and the effect of the Zr content on the microstructure and mechanical property of 15 vol.%TiC/Ti6A14V composites was investigated by XRD, SEM and hardness testing. The results show that when the level of Zr addition is less than 4 wt.%, the morphology of the primary TiC in the composites is dendrite, and the petal-shape, piece-shape or palpus-shape eutectic TiC separates out around the primary TiC. The average size of the primary TiC decreases and the amount of eutectic TiC increases gradually with increasing Zr content. The effects of Zr on morphology of the primary TiC weaken with further addition of Zr. And the hardness (HRC) of composites was obviously increased in the whole range of Zr addition. The refinement mechanism of Zr was attributed to the combined effects of increase in nucleation rate at the constitutionally supercooled zone ahead of the solidification front and reduction in growth rate.

  13. Alterations in mineral properties of zebrafish skeletal bone induced by liliput dtc232 gene mutation

    NASA Astrophysics Data System (ADS)

    Wang, Xiu-Mei; Cui, Fu-Zhai; Ge, Jun; Ma, Chen

    2003-11-01

    The alterations of mineral properties of bone by gene mutation in the zebrafish, which is associated with abnormal bone mineralization and bone diseases, were reported for the first time in this paper. Transmission electron microscope (TEM), Fourier transform infrared microspectroscopy (FTIRM) and thermogravimetric analysis (TGA) were used to investigate the changes in the mineral. Significant variations of the morphologies of the minerals and the mineral/matrix ratio after liliputdtc232(lil) gene mutation have been observed. The morphologies of the minerals, examined by TEM, revealed that the mutated mineral was in bigger size and the shape was block shaped but not plate shaped. The results of FTIRM indicated that the lil mutant zebrafish skeleton exhibited a greater mineral/matrix ratio (phosphate/matrix=4.86±0.28) than that of wild-type zebrafish bone (phosphate/matrix=4.17±0.67), which was confirmed by TGA analysis. Furthermore, the mineral of lil bone became less mature and crystalline with more ion substitutions. And the selected areas electron diffraction (SAED) patterns showed that the main crystal phases of the two type fishes were both hydroxyapatite. In addition, we have discussed the relationship among the mineral properties, nanomechanical properties and biomineralization process.

  14. Cellular basis of morphological variation and temperature-related plasticity in Drosophila melanogaster strains with divergent wing shapes.

    PubMed

    Torquato, Libéria Souza; Mattos, Daniel; Matta, Bruna Palma; Bitner-Mathé, Blanche Christine

    2014-12-01

    Organ shape evolves through cross-generational changes in developmental patterns at cellular and/or tissue levels that ultimately alter tissue dimensions and final adult proportions. Here, we investigated the cellular basis of an artificially selected divergence in the outline shape of Drosophila melanogaster wings, by comparing flies with elongated or rounded wing shapes but with remarkably similar wing sizes. We also tested whether cellular plasticity in response to developmental temperature was altered by such selection. Results show that variation in cellular traits is associated with wing shape differences, and that cell number may play an important role in wing shape response to selection. Regarding the effects of developmental temperature, a size-related plastic response was observed, in that flies reared at 16 °C developed larger wings with larger and more numerous cells across all intervein regions relative to flies reared at 25 °C. Nevertheless, no conclusive indication of altered phenotypic plasticity was found between selection strains for any wing or cellular trait. We also described how cell area is distributed across different intervein regions. It follows that cell area tends to decrease along the anterior wing compartment and increase along the posterior one. Remarkably, such pattern was observed not only in the selected strains but also in the natural baseline population, suggesting that it might be canalized during development and was not altered by the intense program of artificial selection for divergent wing shapes.

  15. Morphology and histology of the alimentary canal of Lygus hesperus (Heteroptera: Cimicomoropha: Miridae)

    USGS Publications Warehouse

    Habibi, J.; Coudron, T.A.; Backus, E.A.; Brandt, S.L.; Wagner, R.M.; Wright, M.K.; Huesing, J.E.

    2008-01-01

    Microdissection and transverse semithin sections were used to perform a light microscopy survey of the gross morphology and cellular anatomy of the alimentary canal, respectively, of Lygus hesperus Knight, a key pest of cotton (Gossypium hirsutum L.), alfalfa (Medicago sativa L.), and other crops. The gross morphology of the alimentary canal showed a relatively unadorned tube compared with other hemipterans, with variably shaped compartments and one small diverticulum. However, the epithelial cell anatomy of the gut was relatively complex, with the midgut having the most diverse structure and cell types. The midgut was typical of the "Lygus-type gut" seen in the older literature, i.e., it consisted of three major regions, the first (descending), second (ascending), and third (descending) ventriculi, with different variants of three major epithelial cell types in each region. Our light microscopy (LM) study suggests that the three cell types are nondifferentiated regenerative cells (which sparsely occurred throughout the midgut but were abundant in the anterior region of the first ventriculus), endocrine cells, and columnar cells. Although the Lygus gut cells strongly resemble those cell types seen in other insects, their identification should be confirmed via transmission electron microscopy to be considered definitive. These cell types differed in the size and opacity of vesicles, geometry of cell surface in the gut lumen, and size, shape, and concentration of brush-border microvilli and location within the gut. Comparison of gut structure in L. hesperus with that of other hemipterans, especially in relation to hemipteran phylogeny and feeding strategies, is discussed.

  16. Embryonic staging and external features of development of the Chimaeroid fish, Callorhinchus milii (Holocephali, Callorhinchidae).

    PubMed

    Didier, Dominique A; LeClair, Elizabeth E; Vanbuskirk, Dana R

    1998-04-01

    The development of Callorhinchus milii, a primitive chondrichthyan fish (Subclass Holocephali) is described in detail based on a complete series of embryos from stage 17 to hatching. The external features of these specimens, in comparison with other chondrichthyan embryos, are used to establish the first staging table for any chimaeroid species. Each stage of C. milii is defined by a suite of morphological characters in addition to total length, including the number of somites, extent of external pigmentation, eye size and shape, head flexure, heart morphology, and size and shape of paired and unpaired fins. Particular attention is given to features of the gill arches and associated structures, including external gill filaments and the opercular flap. Embryos of this species also possess a transient rostral bulb, a feature unique to chimaeroids. Embryological development of Callorhinchus milii is similar to that previously described for sharks and batoids (Subclass Elasmobranchii), including the spiny dogfish, Squalus acanthias, the Japanese bullshark, Heterodontus japonicus, the lesser spotted dogfish, Scyliorhinus canicula, the frill shark, Chlamydoselachus anguineus, the guitarfish, Rhinobatus halavi, and the skate, Raja brachyura. Callorhinchus milii is also similar in overall development to another holocephalan, Hydrolagus colliei. A review of previous staging schemes confirms that early morphological development in all three major chondrichthyan lineages (sharks, batoids, and chimaeras) can be correlated using a common set of stages. A uniform staging system is provided that should prove useful in continuing ontogenetic and phylogenetic studies of this entire clade of fishes. J. Morphol. 236:25-47, 1998. © 1998 Wiley-Liss, Inc. Copyright © 1998 Wiley-Liss, Inc.

  17. Genetic Dissection of Leaf Development in Brassica rapa Using a Genetical Genomics Approach1[W

    PubMed Central

    Xiao, Dong; Wang, Huange; Basnet, Ram Kumar; Zhao, Jianjun; Lin, Ke; Hou, Xilin; Bonnema, Guusje

    2014-01-01

    The paleohexaploid crop Brassica rapa harbors an enormous reservoir of morphological variation, encompassing leafy vegetables, vegetable and fodder turnips (Brassica rapa, ssp. campestris), and oil crops, with different crops having very different leaf morphologies. In the triplicated B. rapa genome, many genes have multiple paralogs that may be regulated differentially and contribute to phenotypic variation. Using a genetical genomics approach, phenotypic data from a segregating doubled haploid population derived from a cross between cultivar Yellow sarson (oil type) and cultivar Pak choi (vegetable type) were used to identify loci controlling leaf development. Twenty-five colocalized phenotypic quantitative trait loci (QTLs) contributing to natural variation for leaf morphological traits, leaf number, plant architecture, and flowering time were identified. Genetic analysis showed that four colocalized phenotypic QTLs colocalized with flowering time and leaf trait candidate genes, with their cis-expression QTLs and cis- or trans-expression QTLs for homologs of genes playing a role in leaf development in Arabidopsis (Arabidopsis thaliana). The leaf gene BRASSICA RAPA KIP-RELATED PROTEIN2_A03 colocalized with QTLs for leaf shape and plant height; BRASSICA RAPA ERECTA_A09 colocalized with QTLs for leaf color and leaf shape; BRASSICA RAPA LONGIFOLIA1_A10 colocalized with QTLs for leaf size, leaf color, plant branching, and flowering time; while the major flowering time gene, BRASSICA RAPA FLOWERING LOCUS C_A02, colocalized with QTLs explaining variation in flowering time, plant architectural traits, and leaf size. Colocalization of these QTLs points to pleiotropic regulation of leaf development and plant architectural traits in B. rapa. PMID:24394778

  18. Comparative ontogeny in humans and chimpanzees: similarities, differences and paradoxes in postnatal growth and development of the skull.

    PubMed

    Bastir, Markus; Rosas, Antonio

    2004-12-01

    The hypothesis of retarded development is a classic and controversial issue in human evolution. It depends directly on the understanding of ontogenetic trajectories and their basic constituents: timing, rate and associated patterns of maturation. In the present study, we applied geometric morphometrics to investigate postnatal ontogeny in human and chimpanzee skulls (N = 302). We evaluated postnatal ontogenetic rates, based on comparisons of properties of size and shape in adults. At different dental ages the percentage of the adult mean size (growth) and adult mean shape (development) was used to quantify patterns of maturation. We found significantly higher levels of ontogenetic maturity in humans than chimpanzees during pre-M1 and M1 eruption. However, during this ontogenetic period the human increments were lower than those of chimpanzees suggesting lower rates. During and after M2-eruption species did not differ in their ontogenetic trajectories. The results indicate that higher prenatal and lower peri- and postnatal maturation rates characterize human ontogeny when compared with chimpanzees. If mandibular ontogeny is considered alone, a paradox was found. Whereas growth maturation proceeded in an expected trajectory continuously approximating 100% adult mean size, developmental maturity was different. After M1-eruption in both species the morphological distance, which had increased before, became reduced again, and reached adult mean shape in a second developmental peak. Such a tendency was found in humans and chimpanzees. This indicates that both size and shape maturation must be considered to understand the complexity of postnatal mandibular ontogeny.

  19. Identification, sexual dimorphism, and allometric effects of three psyllid species of the genus Psyllopsis by geometric morphometric analysis (Hemiptera, Liviidae)

    PubMed Central

    Gushki, Roghayeh Shamsi; Lashkari, Mohammadreza; Mirzaei, Saeid

    2018-01-01

    Abstract Jumping plant lice (Hemiptera: Psylloidea) are considered important vectors of plant diseases and also economically important pests in agriculture and forest ecosystems. Three psyllid species Psyllopsis repens Loginova, 1963, Psyllopsis securicola Loginova, 1963, and Psyllopsis machinosus Loginova, 1963 associated with the ash tree Fraxinus are morphologically very similar. So far, their distinction has been possible only by comparing their male and female genitalia. In this research, forewing shape and size characteristics, sexual dimorphism and their allometric effects, using geometric morphometric analysis, were examined for identification purposes. The results showed significant differences in wing shape and size between the species studied. Based on the results, two species P. machinosus and P. securicola can be differentiated with the vein M1+2, as in P. securicola the vein M1+2 is located between Rs and M3+4 veins, but the vein M1+2 is closer to the vein M3+4 in P. machinosus; also, P. repens can be differentiated from the two species P. machinosus and P. securicola by vein M. Hence, the veins M1+2, M3+4, Rs and M were the most important wing characters for discrimination of the three species, especially in the field. The analysis also showed significant differences in wing shape and size between male and female of the three species, and the allometric analysis showed that significant shape differences still remain in constant size in P. machinosus and P. repens. Geometric changes in the forewings of both sexes for the three species are illustrated. PMID:29674872

  20. Particle morphology dependent superhydrophobicity in treated diatomaceous earth/polystyrene coatings

    NASA Astrophysics Data System (ADS)

    Sedai, Bhishma R.; Alavi, S. Habib; Harimkar, Sandip P.; McCollum, Mark; Donoghue, Joseph F.; Blum, Frank D.

    2017-09-01

    Superhydrophobic surfaces have been prepared from three different types of diatomaceous earth (DE) particles treated with 3-(heptafluoroisopropoxy)propyltrimethoxysilane (HFIP-TMS) and low molecular mass polystyrene. The untreated particles, consisting of CelTix DE (disk shape), DiaFil DE (rod shape) and EcoFlat DE (irregular), were studied using particle size analysis, bulk density, pore volume and surface area analysis (via Brunauer-Emmett-Teller, BET, methods). The treated particles were characterized with thermogravimetric analysis (TGA), contact angles, scanning electron microscopy, profilometry, and FTIR spectroscopy. The minimum amount of silane coupling agent on the DE surfaces required to obtain superhydrophobicity of the particles was determined and found to be dependent on the particle morphology. In the coatings made from different particles with 2.4 wt% HFIP-TMS, the minimum amounts of treated particles (loadings) for superhydrophobicity was determined with the less dense CelTix DE requiring about 30 wt%, DiaFil DE requiring about 40 wt%, and EcoFlat DE each requiring about 60 wt% loading of treated particles.

  1. Synthesis and Characterization of Aqueous Lead Selenide Quantum Dots for Solar Cell Application

    NASA Astrophysics Data System (ADS)

    Albert, Ancy; Sreekala, C. O.; Prabhakaran, Malini

    2018-02-01

    High quality, colloidal lead selenide (PbSe) nanoparticles possessing cube shaped morphology have been successfully synthesized by organometallic synthesis method, using oleic acid (OA) as capping agent. The use of non-coordinating solvent, 1-Octadecene (ODE), during the synthesis results in good quality nanocrystals. Morphology analysis by transmission electron microscopy reveals that cube-shaped nanocrystals with a size range of 10 nm have been produced during the synthesis. The absorption and PL spectra analysis showed an emission peak at 675 nm when excited to a wavelength of 610 nm, further confirmed the formation of PbSe nanocrystals. The surface modification of this colloidal quantum dots was then carried out using L- cysteine ligand, to make them water soluble, for solar cell application. The J-V characteristics study of this PbSe quantum dots solar cell (PbSe QDSC) showed a little power conversion efficiency which intern it shows significant advance toward effective utilization of PbSe nanocrystals sensitized in solar cells.

  2. Nucleation and growth of zinc oxide nanorods directly on metal wire by sonochemical method.

    PubMed

    Rayathulhan, Ruzaina; Sodipo, Bashiru Kayode; Aziz, Azlan Abdul

    2017-03-01

    ZnO nanorods were directly grown on four different wires (silver, nickel, copper, and tungsten) using sonochemical method. Zinc nitrate hexahydrate and hexamethylenetetramine (HMT) were used as precursors. Influence of growth parameters such as precursors' concentration and ultrasonic power on the grown nanorods were determined. The results demonstrated that the precursor concentration affected the growth structure and density of the nanorods. The morphology, distribution, and orientation of nanorods changed as the ultrasonic power changed. Nucleation of ZnO nanorods on the wire occurred at lower ultrasonic power and when the power increased, the formation and growth of ZnO nanorods on the wires were initiated. The best morphology, size, distribution, and orientation of the nanorods were observed on the Ag wire. The presence of single crystal nanorod with hexagonal shaped was obtained. This shape indicates that the ZnO nanorods corresponded to the hexagonal wurtzite structure with growth preferential towards the (002) direction. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Hydrophilic polyurethane matrix promotes chondrogenesis of mesenchymal stem cells☆

    PubMed Central

    Nalluri, Sandeep M.; Krishnan, G. Rajesh; Cheah, Calvin; Arzumand, Ayesha; Yuan, Yuan; Richardson, Caley A.; Yang, Shuying; Sarkar, Debanjan

    2016-01-01

    Segmental polyurethanes exhibit biphasic morphology and can control cell fate by providing distinct matrix guided signals to increase the chondrogenic potential of mesenchymal stem cells (MSCs). Polyethylene glycol (PEG) based hydrophilic polyurethanes can deliver differential signals to MSCs through their matrix phases where hard segments are cell-interactive domains and PEG based soft segments are minimally interactive with cells. These coordinated communications can modulate cell–matrix interactions to control cell shape and size for chondrogenesis. Biphasic character and hydrophilicity of polyurethanes with gel like architecture provide a synthetic matrix conducive for chondrogenesis of MSCs, as evidenced by deposition of cartilage-associated extracellular matrix. Compared to monophasic hydrogels, presence of cell interactive domains in hydrophilic polyurethanes gels can balance cell–cell and cell–matrix interactions. These results demonstrate the correlation between lineage commitment and the changes in cell shape, cell–matrix interaction, and cell–cell adhesion during chondrogenic differentiation which is regulated by polyurethane phase morphology, and thus, represent hydrophilic polyurethanes as promising synthetic matrices for cartilage regeneration. PMID:26046282

  4. Turning maneuvers in sharks: Predicting body curvature from axial morphology.

    PubMed

    Porter, Marianne E; Roque, Cassandra M; Long, John H

    2009-08-01

    Given the diversity of vertebral morphologies among fishes, it is tempting to propose causal links between axial morphology and body curvature. We propose that shape and size of the vertebrae, intervertebral joints, and the body will more accurately predict differences in body curvature during swimming rather than a single meristic such as total vertebral number alone. We examined the correlation between morphological features and maximum body curvature seen during routine turns in five species of shark: Triakis semifasciata, Heterodontus francisci, Chiloscyllium plagiosum, Chiloscyllium punctatum, and Hemiscyllium ocellatum. We quantified overall body curvature using three different metrics. From a separate group of size-matched individuals, we measured 16 morphological features from precaudal vertebrae and the body. As predicted, a larger pool of morphological features yielded a more robust prediction of maximal body curvature than vertebral number alone. Stepwise linear regression showed that up to 11 features were significant predictors of the three measures of body curvature, yielding highly significant multiple regressions with r(2) values of 0.523, 0.537, and 0.584. The second moment of area of the centrum was always the best predictor, followed by either centrum length or transverse height. Ranking as the fifth most important variable in three different models, the body's total length, fineness ratio, and width were the most important non-vertebral morphologies. Without considering the effects of muscle activity, these correlations suggest a dominant role for the vertebral column in providing the passive mechanical properties of the body that control, in part, body curvature during swimming. (c) 2009 Wiley-Liss, Inc.

  5. Au nanostructure arrays for plasmonic applications: annealed island films versus nanoimprint lithography

    NASA Astrophysics Data System (ADS)

    Lopatynskyi, Andrii M.; Lytvyn, Vitalii K.; Nazarenko, Volodymyr I.; Guo, L. Jay; Lucas, Brandon D.; Chegel, Volodymyr I.

    2015-03-01

    This paper attempts to compare the main features of random and highly ordered gold nanostructure arrays (NSA) prepared by thermally annealed island film and nanoimprint lithography (NIL) techniques, respectively. Each substrate possesses different morphology in terms of plasmonic enhancement. Both methods allow such important features as spectral tuning of plasmon resonance position depending on size and shape of nanostructures; however, the time and cost is quite different. The respective comparison was performed experimentally and theoretically for a number of samples with different geometrical parameters. Spectral characteristics of fabricated NSA exhibited an expressed plasmon peak in the range from 576 to 809 nm for thermally annealed samples and from 606 to 783 nm for samples prepared by NIL. Modelling of the optical response for nanostructures with typical shapes associated with these techniques (parallelepiped for NIL and semi-ellipsoid for annealed island films) was performed using finite-difference time-domain calculations. Mathematical simulations have indicated the dependence of electric field enhancement on the shape and size of the nanoparticles. As an important point, the distribution of electric field at so-called `hot spots' was considered. Parallelepiped-shaped nanoparticles were shown to yield maximal enhancement values by an order of magnitude greater than their semi-ellipsoid-shaped counterparts; however, both nanoparticle shapes have demonstrated comparable effective electrical field enhancement values. Optimized Au nanostructures with equivalent diameters ranging from 85 to 143 nm and height equal to 35 nm were obtained for both techniques, resulting in the largest electrical field enhancement. The application of island film thermal annealing method for nanochips fabrication can be considered as a possible cost-effective platform for various surface-enhanced spectroscopies; while the NIL-fabricated NSA looks like more effective for sensing of small-size objects.

  6. The influence of digit size and proportions on dexterity during cold exposure.

    PubMed

    Payne, Stephanie; Macintosh, Alison; Stock, Jay

    2018-04-20

    The current study investigated whether size and proportions of the hands and digits affect dexterity during severe cold exposure. As wide hands are known to lose less heat than narrow hands, and narrow digits are associated with greater dexterity, this study aimed to test whether there was a direct trade-off between dexterity and thermoregulation that shapes hand morphology. Participants (25 women, 15 men) carried out the Purdue Pegboard test before and after a 3-min ice-water immersion of the hand. Their hand length, hand width, digit lengths, and digit widths were measured using standard anthropometric methods. Wide first and third digits associated with significantly reduced dexterity after immersion relative to individuals with narrower first and third digits. Second digit width positively correlated with average digit temperature after immersion. Hand length and hand width did not influence dexterity. The current study suggests that digit width influences dexterity in cold conditions, reflecting patterns found at room temperature. Hand and digit morphology may be the product of two significant constraints on the hand: dexterity and thermoregulation. In cold conditions, hand morphology appears to be predominantly constrained by thermal stress, at the expense of dexterity. This may have important implications for interpreting the morphology of extinct and extant hominins. © 2018 Wiley Periodicals, Inc.

  7. Size and shape of uniform particles precipitated in homogeneous solutions

    NASA Astrophysics Data System (ADS)

    Sevonkaev, Igor V.

    The assembly of nanosize crystals into larger uniform colloids is a fundamental process that plays a critical role in the formation of a very broad range of fine-particles used in numerous applications in technology, medicine, and national security. It is widely accepted that, along with size, in most of these applications the shape of the particles represents a critical factor. In the current research, we investigate the size and shape control of uniform particles prepared by precipitation in homogeneous solutions. In the first---theoretical---part a combinational mechanism of the shape control during particle growth was proposed and analyzed numerically. The main finding of our simulation is that a proper balance of two processes, preferential attachment of transported monomers at the protruding features of the growing cluster and monomer rearrangement at the cluster surface, can yield a well-defined particle shape that persist for sizes much larger than the original seed over a large interval of time. In the experimental part, three chemically simple systems were selected MgF2, NaMgF3, and PbS for defining and evaluating the key parameters of the shape and size control of the precipitates. Thus, uniform dispersions of particles of different morphologies (spherical, cubic, platelet, and prismatic) were prepared by precipitation in aqueous solutions. The mechanisms of the formation of the resulting particles of different shapes are explained by the role of the pH, temperature, solubility, and ionic strength. Stages of particles growth were evaluated on short and long time scales, winch allowed to propose multistage mechanisms of NaMgF3 growth and estimate induction time and critical nuclei size for MgF2. In addition, for prospective numerical modeling the surface tensions of spherical and platelet particles of MgF2 were evaluated from the X-ray data by a lattice parameter change method. Also, a new method for the evaluation of the variation in the density distribution in colloidal spherical particles was proposed. This method utilizes transmission electron microscopy without high resolution mode and processes acquired images. Suggested method eliminates the dependency of the image contrast on sample crystallinity. The advantage of such approach manifested by the short time sample preparation, fast instrument tune-up, rapid image acquisition and analysis, all of which shortens the processing time.

  8. The evolution of bat vestibular systems in the face of potential antagonistic selection pressures for flight and echolocation.

    PubMed

    Davies, Kalina T J; Bates, Paul J J; Maryanto, Ibnu; Cotton, James A; Rossiter, Stephen J

    2013-01-01

    The vestibular system maintains the body's sense of balance and, therefore, was probably subject to strong selection during evolutionary transitions in locomotion. Among mammals, bats possess unique traits that place unusual demands on their vestibular systems. First, bats are capable of powered flight, which in birds is associated with enlarged semicircular canals. Second, many bats have enlarged cochleae associated with echolocation, and both cochleae and semicircular canals share a space within the petrosal bone. To determine how bat vestibular systems have evolved in the face of these pressures, we used micro-CT scans to compare canal morphology across species with contrasting flight and echolocation capabilities. We found no increase in canal radius in bats associated with the acquisition of powered flight, but canal radius did correlate with body mass in bat species from the suborder Yangochiroptera, and also in non-echolocating Old World fruit bats from the suborder Yinpterochiroptera. No such trend was seen in members of the Yinpterochiroptera that use laryngeal echolocation, although canal radius was associated with wing-tip roundedness in this group. We also found that the vestibular system scaled with cochlea size, although the relationship differed in species that use constant frequency echolocation. Across all bats, the shape of the anterior and lateral canals was associated with large cochlea size and small body size respectively, suggesting differential spatial constraints on each canal depending on its orientation within the skull. Thus in many echolocating bats, it seems that the combination of small body size and enlarged cochlea together act as a principal force on the vestibular system. The two main groups of echolocating bats displayed different canal morphologies, in terms of size and shape in relation to body mass and cochlear size, thus suggesting independent evolutionary pathways and offering tentative support for multiple acquisitions of echolocation.

  9. The Evolution of Bat Vestibular Systems in the Face of Potential Antagonistic Selection Pressures for Flight and Echolocation

    PubMed Central

    Davies, Kalina T. J.; Bates, Paul J. J.; Maryanto, Ibnu; Cotton, James A.; Rossiter, Stephen J.

    2013-01-01

    The vestibular system maintains the body’s sense of balance and, therefore, was probably subject to strong selection during evolutionary transitions in locomotion. Among mammals, bats possess unique traits that place unusual demands on their vestibular systems. First, bats are capable of powered flight, which in birds is associated with enlarged semicircular canals. Second, many bats have enlarged cochleae associated with echolocation, and both cochleae and semicircular canals share a space within the petrosal bone. To determine how bat vestibular systems have evolved in the face of these pressures, we used micro-CT scans to compare canal morphology across species with contrasting flight and echolocation capabilities. We found no increase in canal radius in bats associated with the acquisition of powered flight, but canal radius did correlate with body mass in bat species from the suborder Yangochiroptera, and also in non-echolocating Old World fruit bats from the suborder Yinpterochiroptera. No such trend was seen in members of the Yinpterochiroptera that use laryngeal echolocation, although canal radius was associated with wing-tip roundedness in this group. We also found that the vestibular system scaled with cochlea size, although the relationship differed in species that use constant frequency echolocation. Across all bats, the shape of the anterior and lateral canals was associated with large cochlea size and small body size respectively, suggesting differential spatial constraints on each canal depending on its orientation within the skull. Thus in many echolocating bats, it seems that the combination of small body size and enlarged cochlea together act as a principal force on the vestibular system. The two main groups of echolocating bats displayed different canal morphologies, in terms of size and shape in relation to body mass and cochlear size, thus suggesting independent evolutionary pathways and offering tentative support for multiple acquisitions of echolocation. PMID:23637943

  10. The rise and fall of stellar across the peak of cosmic star formation history: effects of mergers versus diffuse stellar mass acquisition

    NASA Astrophysics Data System (ADS)

    Welker, C.; Dubois, Y.; Devriendt, J.; Pichon, C.; Kaviraj, S.; Peirani, S.

    2017-02-01

    Building galaxy merger trees from a state-of-the-art cosmological hydrodynamical simulation, Horizon-AGN, we perform a statistical study of how mergers and diffuse stellar mass acquisition processes drive galaxy morphologic properties above z > 1. By diffuse mass acquisition here, we mean both accretion of stars by unresolved mergers (relative stellar mass growth smaller than 4.5 per cent) as well as in situ star formation when no resolved mergers are detected along the main progenitor branch of a galaxy. We investigate how stellar densities, galaxy sizes and galaxy morphologies (defined via shape parameters derived from the inertia tensor of the stellar density) depend on mergers of different mass ratios. We investigate how stellar densities, effective radii and shape parameters derived from the inertia tensor depend on mergers of different mass ratios. We find strong evidence that diffuse stellar accretion and in situ formation tend to flatten small galaxies over cosmic time, leading to the formation of discs. On the other hand, mergers, and not only the major ones, exhibit a propensity to puff up and destroy stellar discs, confirming the origin of elliptical galaxies. We confirm that mergers grow galaxy sizes more efficiently than diffuse processes (r_{0.5}∝ M_s^{0.85} and r_{0.5}∝ M_s^{0.1} on average, respectively) and we also find that elliptical galaxies are more susceptible to grow in size through mergers than disc galaxies with a size-mass evolution r_{0.5}∝ M_s^{1.2} instead of r_{0.5}∝ M_s^{-0.5}-M^{0.5} for discs depending on the merger mass ratio. The gas content drives the size-mass evolution due to merger with a faster size growth for gas-poor galaxies r_{0.5}∝ M_s2 than for gas-rich galaxies r0.5 ∝ Ms.

  11. Preparation and characterization of superfine ammonium perchlorate (AP) crystals through ceramic membrane anti-solvent crystallization

    NASA Astrophysics Data System (ADS)

    Ma, Zhenye; Li, Cheng; Wu, Rujun; Chen, Rizhi; Gu, Zhenggui

    2009-10-01

    In this paper, a novel ceramic membrane anti-solvent crystallization (CMASC) method was proposed for the safe and rapid preparation ammonium perchlorate (AP) crystals, in which the acetone and ethyl acetate were chosen as solvent and anti-solvent, respectively. Comparing with the conventional liquid anti-solvent crystallization (LASC), CMASC which successfully introduces ceramic membrane with regular pore structure to the LASC as feeding medium, is favorable to control the rate of feeding rate and, therefore, to obtain size and morphology controllable AP. Several kinds of micro-sized AP particles with different morphology were obtained including polyhedral-like, quadrate-like to rod-like. The effect of processing parameters on the crystal size and shape of AP crystals such as volume ratio of anti-solvent to solvent, feeding pressure and crystallization temperature were investigated. It is found that higher volume ratio of anti-solvent to solvent, higher feeding pressure and higher temperature result in smaller particle size. Scaning electron microscopy (SEM) and X-ray diffraction (XRD) were used to characterize the resulting AP crystals. The nucleation and growth kinetic of the resulting AP crystals were also discussed.

  12. Hemocytes of Rhipicephalus sanguineus (Acari: Ixodidae): Characterization, Population Abundance, and Ultrastructural Changes Following Challenge with Leishmania infantum.

    PubMed

    Feitosa, A P S; Alves, L C; Chaves, M M; Veras, D L; Silva, E M; Aliança, A S S; França, I R S; Gonçalves, G G A; Lima-Filho, J L; Brayner, F A

    2015-11-01

    Few studies have examined the cellular immune response of ticks, and further research on the characterization of the hemocytes of ticks is required, particularly on those of Rhipicephalus sanguineus (Latreille) because of the medical and veterinary importance of this tick. The aims of this study were to characterize the morphology and the ultrastructure of the different types of hemocytes of adult R. sanguineus and to determine the population abundance and the ultrastructural changes in the hemocytes of ticks infected with Leishmania infantum. The hemocytes were characterized through light and transmission electron microscopy. Within the variability of circulating cells in the hemolymph of adult R. sanguineus, five cell types were identified, which were the prohemocytes, plasmatocytes, granulocytes, spherulocytes, and adipohemocytes. The prohemocytes were the smallest cells found in the hemolymph. The plasmatocytes had polymorphic morphology with vesicles and cytoplasmic projections. The granulocytes had an elliptical shape with the cytoplasm filled with granules of different sizes and electrodensities. The spherulocytes were characterized by several spherules of uniform shapes and sizes that filled the entire cytoplasm, whereas the adipohemocytes had an irregular shape with multiple lipid inclusions that occupied almost the entire cytoplasmic space. The total counts of the hemocyte population increased in the group that was infected with L. infantum. Among the different cell types, the numbers increased and the ultrastructural changes occurred in the granulocytes and the plasmatocytes in the infected group of ticks. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  13. Drug particle size influence on enteric beads produced by a droplet extrusion/precipitation method.

    PubMed

    Cerdeira, A M; Gouveia, L F; Goucha, P; Almeida, A J

    2000-01-01

    The influence of drug particle size on the production of enteric beads by a polymer precipitation technique was investigated. Drug particle dimensions are known to play an important role in most microencapsulation techniques. Bead morphology was greatly influenced by drug particle size, and spherical shaped beads could only be obtained after size reduction of nimesulide crystals. This is confirmed by the angle of repose measurements, which show a significant decrease in theta values when beads are formulated with smaller drug particles. Furthermore, results show that drug encapsulation efficiency and in vitro drug release rates are also greatly dependent on both drug particle size and drug/polymer ratio in the initial suspension. Preparations containing 10.2 microm drug particles show a two-fold increase in the release rates when compared to those prepared with 40 microm particles.

  14. Influence of morphology and hemodynamic factors on rupture of multiple intracranial aneurysms: matched-pairs of ruptured-unruptured aneurysms located unilaterally on the anterior circulation.

    PubMed

    Zhang, Ying; Yang, Xinjian; Wang, Yang; Liu, Jian; Li, Chuanhui; Jing, Linkai; Wang, Shengzhang; Li, Haiyun

    2014-12-31

    The authors evaluated the impact of morphological and hemodynamic factors on the rupture of matched-pairs of ruptured-unruptured intracranial aneurysms on one patient's ipsilateral anterior circulation with 3D reconstruction model and computational fluid dynamic method simulation. 20 patients with intracranial aneurysms pairs on the same-side of anterior circulation but with different rupture status were retrospectively collected. Each pair was divided into ruptured-unruptured group. Patient-specific models based on their 3D-DSA images were constructed and analyzed. The relative locations, morphologic and hemodynamic factors of these two groups were compared. There was no significant difference in the relative bleeding location. The morphological factors analysis found that the ruptured aneurysms more often had irregular shape and had significantly higher maximum height and aspect ratio. The hemodynamic factors analysis found lower minimum wall shear stress (WSSmin) and more low-wall shear stress-area (LSA) in the ruptured aneurysms than that of the unruptured ones. The ruptured aneurysms more often had WSSmin on the dome. Intracranial aneurysms pairs with different rupture status on unilateral side of anterior circulation may be a good disease model to investigate possible characteristics linked to rupture independent of patient characteristics. Irregular shape, larger size, higher aspect ratio, lower WSSmin and more LSA may indicate a higher risk for their rupture.

  15. Morphology-controlled electrodeposition of Cu{sub 2}O microcrystalline particle films for application in photocatalysis under sunlight

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wu, Guodong; Zhai, Wei; Sun, Fengqiang, E-mail: fqsun@scnu.edu.cn

    2012-12-15

    Graphical abstract: Display Omitted Highlights: ► PEG was used to electro-deposit Cu{sub 2}O microcrystalline particle films. ► Morphologies of Cu{sub 2}O microcrystals could be controlled by the amount of PEG. ► The films showed regularly varied photocatalytic activities under sunlight. ► The films could be recycled and showed stable activities. -- Abstract: Morphology-controlled Cu{sub 2}O microcrystalline particle films had been successfully electrodeposited on tin-doped indium oxide glass substrates in CuSO{sub 4} solutions containing different amounts of polyethylene glycol (PEG) additives. With an increase of PEG, microcrystals gradually changed from irregular shapes to cubes, octahedrons, and spherical shapes. Sizes increasingly becamemore » smaller with an increase of PEG under the same deposition time. These films had been first used as recyclable photocatalysts and showed excellent and photocatalytic activities in photodegradation of methylene blue (MB) under sunlight. Activities were regularly varied relative to the morphologies of films controlled by the amount of PEG and could be further enhanced by adding a little amount of hydrogen peroxide in the MB solution. The method for controllable preparation of Cu{sub 2}O microcrystals with photocatalytic activities was simple and inexpensive. The as-prepared particle films could also be used in photodegradation of many other pollutants under sunlight.« less

  16. A quantitative framework for flower phenotyping in cultivated carnation (Dianthus caryophyllus L.).

    PubMed

    Chacón, Borja; Ballester, Roberto; Birlanga, Virginia; Rolland-Lagan, Anne-Gaëlle; Pérez-Pérez, José Manuel

    2013-01-01

    Most important breeding goals in ornamental crops are plant appearance and flower characteristics where selection is visually performed on direct offspring of crossings. We developed an image analysis toolbox for the acquisition of flower and petal images from cultivated carnation (Dianthus caryophyllus L.) that was validated by a detailed analysis of flower and petal size and shape in 78 commercial cultivars of D. caryophyllus, including 55 standard, 22 spray and 1 pot carnation cultivars. Correlation analyses allowed us to reduce the number of parameters accounting for the observed variation in flower and petal morphology. Convexity was used as a descriptor for the level of serration in flowers and petals. We used a landmark-based approach that allowed us to identify eight main principal components (PCs) accounting for most of the variance observed in petal shape. The effect and the strength of these PCs in standard and spray carnation cultivars are consistent with shared underlying mechanisms involved in the morphological diversification of petals in both subpopulations. Our results also indicate that neighbor-joining trees built with morphological data might infer certain phylogenetic relationships among carnation cultivars. Based on estimated broad-sense heritability values for some flower and petal features, different genetic determinants shall modulate the responses of flower and petal morphology to environmental cues in this species. We believe our image analysis toolbox could allow capturing flower variation in other species of high ornamental value.

  17. Tooth wear and dentoalveolar remodeling are key factors of morphological variation in the Dmanisi mandibles

    PubMed Central

    Margvelashvili, Ann; Zollikofer, Christoph P. E.; Lordkipanidze, David; Peltomäki, Timo; Ponce de León, Marcia S.

    2013-01-01

    The Plio-Pleistocene hominin sample from Dmanisi (Georgia), dated to 1.77 million years ago, is unique in offering detailed insights into patterns of morphological variation within a paleodeme of early Homo. Cranial and dentoalveolar morphologies exhibit a high degree of diversity, but the causes of variation are still relatively unexplored. Here we show that wear-related dentoalveolar remodeling is one of the principal mechanisms causing mandibular shape variation in fossil Homo and in modern human hunter–gatherer populations. We identify a consistent pattern of mandibular morphological alteration, suggesting that dental wear and compensatory remodeling mechanisms remained fairly constant throughout the evolution of the genus Homo. With increasing occlusal and interproximal tooth wear, the teeth continue to erupt, the posterior dentition tends to drift in a mesial direction, and the front teeth become more upright. The resulting changes in dentognathic size and shape are substantial and need to be taken into account in comparative taxonomic analyses of isolated hominin mandibles. Our data further show that excessive tooth wear eventually leads to a breakdown of the normal remodeling mechanisms, resulting in dentognathic pathologies, tooth loss, and loss of masticatory function. Complete breakdown of dentognathic homeostasis, however, is unlikely to have limited the life span of early Homo because this effect was likely mediated by the preparation of soft foods. PMID:24101504

  18. A family of membrane-shaping proteins at ER subdomains regulates pre-peroxisomal vesicle biogenesis.

    PubMed

    Joshi, Amit S; Huang, Xiaofang; Choudhary, Vineet; Levine, Tim P; Hu, Junjie; Prinz, William A

    2016-11-21

    Saccharomyces cerevisiae contains three conserved reticulon and reticulon-like proteins that help maintain ER structure by stabilizing high membrane curvature in ER tubules and the edges of ER sheets. A mutant lacking all three proteins has dramatically altered ER morphology. We found that ER shape is restored in this mutant when Pex30p or its homologue Pex31p is overexpressed. Pex30p can tubulate membranes both in cells and when reconstituted into proteoliposomes, indicating that Pex30p is a novel ER-shaping protein. In contrast to the reticulons, Pex30p is low abundance, and we found that it localizes to subdomains in the ER. We show that these ER subdomains are the sites where most preperoxisomal vesicles (PPVs) are generated. In addition, overproduction or deletion of Pex30p or Pex31p alters the size, shape, and number of PPVs. Our findings suggest that Pex30p and Pex31p help shape and generate regions of the ER where PPV biogenesis occurs.

  19. Brief Communication: Buoyancy-Induced Differences in Soot Morphology

    NASA Technical Reports Server (NTRS)

    Ku, Jerry C.; Griffin, Devon W.; Greenberg, Paul S.; Roma, John

    1995-01-01

    Reduction or elimination of buoyancy in flames affects the dominant mechanisms driving heat transfer, burning rates and flame shape. The absence of buoyancy produces longer residence times for soot formation, clustering and oxidation. In addition, soot pathlines are strongly affected in microgravity. We recently conducted the first experiments comparing soot morphology in normal and reduced-gravity laminar gas jet diffusion flames. Thermophoretic sampling is a relatively new but well-established technique for studying the morphology of soot primaries and aggregates. Although there have been some questions about biasing that may be induced due to sampling, recent analysis by Rosner et al. showed that the sample is not biased when the system under study is operating in the continuum limit. Furthermore, even if the sampling is preferentially biased to larger aggregates, the size-invariant premise of fractal analysis should produce a correct fractal dimension.

  20. Mineral Biomarkers in Martian Meteorite Allan Hills 84001?

    NASA Technical Reports Server (NTRS)

    Thomas-Keprta, K. L.; Bazylinski, D. A.; Wentworth, S. J.; McKay, D. S.; Golden, D. C.; Gibson, E. K., Jr.; Romanek, C. S.

    1998-01-01

    The occurrence of fine-grained magnetite in the Fe-rich rims surrounding carbonate globules in the martian meteorite ALH84001, originally described in , have been proposed as fossil remains of primitive martian organisms. Here we report observations on size and shape distributions of magnetites from ALH84001 and compare them to biogenic and inorganic magnetite crystals of terrestrial origin. While some magnetite morphology is not unequivocally diagnostic for its biogenicity, such as cubodial forms of magnetite, which are common in inorganically formed magnetites, other morphologies of magnetite (parallel-epiped or elongated prismatic and arrowhead forms) are more likely signatures of biogenic activity. Some ALH 84001 magnetite particles described below have unique morphology and length-to-width ratios that are indistinguishable from a variety of terrestrial biogenic magnetite and distinct from all known inorganic forms of magnetite.

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