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Sample records for sja muutuv ngu

  1. 32 CFR 516.17 - SJA or legal adviser procedures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true SJA or legal adviser procedures. 516.17 Section 516.17 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Reporting Legal Proceedings to HQDA § 516.17 SJA or legal...

  2. 32 CFR 516.17 - SJA or legal adviser procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... procedures. (a) Immediate notice to HQDA. When an SJA or legal adviser learns of litigation in which the... following: (1) Title or style of the proceeding. (2) Full names and addresses of the parties. (3) Tribunal...

  3. 32 CFR 516.17 - SJA or legal adviser procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... procedures. (a) Immediate notice to HQDA. When an SJA or legal adviser learns of litigation in which the... following: (1) Title or style of the proceeding. (2) Full names and addresses of the parties. (3) Tribunal...

  4. 32 CFR 516.17 - SJA or legal adviser procedures.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... procedures. (a) Immediate notice to HQDA. When an SJA or legal adviser learns of litigation in which the... following: (1) Title or style of the proceeding. (2) Full names and addresses of the parties. (3) Tribunal...

  5. 32 CFR 516.17 - SJA or legal adviser procedures.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... procedures. (a) Immediate notice to HQDA. When an SJA or legal adviser learns of litigation in which the... following: (1) Title or style of the proceeding. (2) Full names and addresses of the parties. (3) Tribunal...

  6. Analysis of the "-sja" Passive of Russian Verbs of Governing and Wanting as a Conceptual Integration

    ERIC Educational Resources Information Center

    Min, Junghee

    2011-01-01

    This dissertation represents a study of the formation of "-sja" passives of Russian verbs of governing and wanting. I explore five imperfective verbs of governing: "zavedovat" "manage"; "komandovat" "command"; "pravit" "govern"; "rukovdit" "direct"; and "upravljat" "manage"; as well as four imperfective verbs of wanting: "iskat" "seek"; "trebovat"…

  7. Analysis of the "-sja" Passive of Russian Verbs of Governing and Wanting as a Conceptual Integration

    ERIC Educational Resources Information Center

    Min, Junghee

    2011-01-01

    This dissertation represents a study of the formation of "-sja" passives of Russian verbs of governing and wanting. I explore five imperfective verbs of governing: "zavedovat" "manage"; "komandovat" "command"; "pravit" "govern"; "rukovdit" "direct"; and "upravljat" "manage"; as well as four imperfective verbs of wanting: "iskat" "seek"; "trebovat"…

  8. SJA Deskbook

    DTIC Science & Technology

    1991-10-01

    security zones, beyond the territorial sea, of varying breadth in which they purport to regulate the activities of warships and military aircraft of...given time, prohibitions on various operational activities , or complete exclusion. These restrictions are not recognized under international law...under bareboat charter, and assigned to MSC); (2) the National Defense Reserve Fleet and the Ready Reserve Force (RRF) (when activated and assigned to

  9. Fermentation, isolation, purification and biological activity of SJA-95, a heptaene polyene macrolide antibiotic produced by the Streptomyces sp. strain S24.

    PubMed

    Naik, Suresh R; Desai, Sandhya K; Nanda, Rabindra K; Narayanan, Mangalam S

    2007-01-01

    A new strain, Streptomyces sp. S24 was isolated from a soil sample collected from Japan. Preliminary morphological, cultural, physiological and biochemical studies on this strain were carried out. Under submerged culture conditions the strain produced heptaene polyene macrolide (SJA-95), which elicits promising antifungal activity in vitro against yeasts, filamentous fungi including clinical isolates and plant pathogens. Its antifungal activity is comparable to that of hamycin and amphotericin B. SJA-95 was found to be toxic when given by the parenteral route in mice and not absorbed by the oral route like other polyene heptaene macrolides. The physicochemical data, spectral studies and elemental analysis suggest that SJA-95 is a polyene macrolide antibiotic. However, the chemical structure needs to be elucidated by further spectroscopic studies viz. 13C NMR, FEB-MS, EL MS and other tests.

  10. Study of immunological aspects of aspergillosis in mice and effect of polyene macrolide antibiotic (SJA-95) and IFN-γ: a possible role of IFN-γ as an adjunct in antifungal therapy.

    PubMed

    Naik, Suresh R; Thakare, Vishnu N; Desai, Sandhya K; Rahalkar, Prabhakar R

    2011-12-30

    New polyene macrolide antibiotic SJA-95 in free as well as liposomal (lip.) forms, with and without interferon-γ (IFN-γ) was studied in mice model of aspergillosis using biological and biochemical parameters viz. colony forming units (CFU) in liver, spleen, kidney, lung and brain, and serum IgG, and interleukin-4 (IL-4). Treatment with free and lip SJA-95 along with IFN-γ prolonged the survival time, reduced CFU in vital organs, decreased serum IgG and IL-4 levels. SJA-95 lip form showed greater antifungal activity as compared to free form. The combined treatment of lip SJA-95 with IFN-γ showed further enhancement in antifungal activity of SJA-95 (lip). The present experimental findings demonstrated IFN-γ might act as a potent modulator in immune reaction during fungal infection and can be a useful adjunctive in antifungal therapy in the management of deep seated systemic mycoses.

  11. Retention of clinical trial participants in a study of nongonococcal urethritis (NGU), a sexually transmitted infection in men.

    PubMed

    Lee, Jeannette Y; Lensing, Shelly Y; Schwebke, Jane R

    2012-07-01

    Nongonococcal urethritis (NGU), an inflammation of the urethra not caused by gonorrhea, is the most common urethritis syndrome seen in men in the United States. It is a sexually transmitted infection commonly caused by Chlamydia trachomatis, a pathogen which occurs more frequently in African-American men compared to white men. The purpose of this study was to investigate factors related to retention of study participants in a randomized, double-blinded clinical trial that evaluated four treatment regimens for the treatment of NGU. After the one-week treatment period, follow-up visits were scheduled during days 15-19 and days 35-45. Participants were phoned prior to scheduled appointments to encourage attendance, and contacted after missed appointments to reschedule their clinic visits. Of the 305 male study participants, 298 (98%) were African-American, 164 (54%) were 25 years of age or younger, and 80 (31%) had a post-secondary school education. The overall retention rate was 75%. Factors associated with study completion were educational level attained and clinical center. Participants with higher levels of education were more likely to complete the study. Clinical centers with the highest retention rates also provided the highest monetary incentives for participation. The retention rate for this study suggests that strategies are needed for improving the proportion of study participants that complete a clinical trial among young men with a sexually transmitted disease. These strategies may include increasing contacts with study participants to remind them of scheduled study visits using text messaging or social media and the use of financial incentives.

  12. B cells that simultaneously express surface IgM and IgE in Nippostrongylus brasiliensis-infected SJA/9 mice do not provide evidence for isotype switching without gene deletion.

    PubMed Central

    Katona, I M; Urban, J F; Finkelman, F D

    1985-01-01

    Recently, it has been reported that in SJA/9 mice infected with Nippostrongylus brasiliensis there are increased numbers of lymphoid cells positive for surface IgM and IgE (sIgM+ and sIgE+) even though they fail to secrete IgE, that both the sIgM and sIgE on these cells are intrinsic, and that there has been no deletion of genes for the Ig heavy chain constant region in these cells. These observations support a nondeletional model for Ig isotype switching. We have now reexamined the nature of sIgE on sIgE+ spleen and mesenteric lymph node cells of N. brasiliensis-infected SJA/9 mice, and the following observations lead us to believe that this sIgE is cytophilic rather than intrinsic: (i) Only approximately 50% of the N. brasiliensis-infected SJA/9 mice have detectable percentages of sIgE+ lymphoid cells. All mice with detectable sIgE+ lymphocytes have lymphocytes positive for intracytoplasmic IgE (cIgE+) and secrete IgE in vitro, while cIgE+ cells and IgE secretion are absent from N. brasiliensis-infected SJA/9 mice that lack sIgE+ cells. (ii) SJA/9 B lymphocytes have receptors for IgE: expression of these receptors is increased in N. brasiliensis-infected mice that have sIgE+ lymphocytes, but not in infected SJA/9 mice that lack sIgE+ lymphocytes. (iii) Treatment of sIgM+ sIgD+ sIgE+ cells for 1 min with dilute acid removes most sIgE but does not affect expression of sIgM or sIgD. (iv) The removal of mouse IgE from sIgE+ B cells facilitates the binding of exogenous rat IgE. (v) The small amount of sIgE that is reexpressed during a period of in vitro culture after acid treatment is blocked by inclusion of exogenous rat IgE in the culture medium. These observations show that most sIgM+ sIgE+ B cells in N. brasiliensis-infected SJA/9 mice do not express intrinsic sIgE; thus studies using these cells to determine mechanisms of Ig isotype switching are inconclusive. PMID:3155861

  13. Azithromycin as the first-line treatment of non-gonococcal urethritis (NGU): a study of follow-up rates, contact attendance and patients' treatment preference.

    PubMed

    Carlin, E M; Barton, S E

    1996-01-01

    To identify any differences in follow-up rates or sexual contact attendance rates in men presenting with non-gonococcal urethritis (NGU) after treatment by single dose azithromycin rather than longer standard duration therapies and to identify patients' treatment preferences. A prospective study was performed on 200 consecutive men attending a genito-urinary medicine (GUM) clinic with new episode, microscopically confirmed NGU. The first 100 patients were treated with standard duration therapy (Group S) whilst the second 100 patients received a single 1 g oral dose of azithromycin (Group A). Patient-led contact tracing was arranged and patients were asked to return for review when a test of cure was performed, contact attendance noted and the patient's treatment preference ascertained. Both groups were predominantly heterosexual and over 60% gave a history of previous sexually transmitted disease (STD). There were no significant differences in efficacy between Groups S and A. However, the index follow-up rate and percentage of traceable sexual contacts attending was higher in Group A. In both groups contacts of homosexual men were more likely to attend the GUM clinic. More additional visits were made by Group S due to mislaid medication or compliance problems. Over 70% of patients questioned expressed a preference for single dose therapy. Single dose therapy with 1 g of azithromycin is as efficacious as longer duration therapies with advantages in patient follow-up rates and contact attendance and for the majority of patients would be their treatment of choice. A cost analysis supports the practical application of this regimen.

  14. Novel multiplex real-time PCR system using the SNP technology for the simultaneous diagnosis of Chlamydia trachomatis, Ureaplasma parvum and Ureaplasma urealyticum and genetic typing of serovars of C. trachomatis and U. parvum in NGU.

    PubMed

    Tang, Jingfeng; Zhou, Li; Liu, Xiaoying; Zhang, Changming; Zhao, Youyun; Wang, Yefu

    2011-02-01

    To explore the possibilities of a novel multiplex real-time PCR system for rapid diagnosis, genetic typing of serovars and clinical application in NGU, we developed a multiplex real-time PCR system for the simultaneous diagnosis of Chlamydia trachomatis, Ureaplasma parvum and Ureaplasma urealyticum and molecular detection of serovars of C. trachomatis and U. parvum in NGU using the SNP technology and TaqMan-LNA probe. In 57 pathogen-positive clinical specimens, we identified the following C. trachomatis serovars: D (20.05%, 12/57), E (36.84%, 21/57), F (19.30%, 11/57), G (8.77%, 5/57), H (5.26%, 3/57), J (3.51%, 2/57), and K (5.26%, 3/57). In 115 pathogen-positive clinical specimens, we identified the following U. parvum serovars: 1 (0.87%, 2/115), 3 (55.65%, 64/115), 6 (20.87%, 24/115) and 14 (21.74%, 25/115). Our fast pathogen diagnosis and serotyping assay using real-time TaqMan-LNA PCR may improve our ability to study the pathogenesis and epidemiology of NGU.

  15. Numerical Simulations of Jet Mixing Enhancement in a Round Jet Exhaust Using SJA

    NASA Astrophysics Data System (ADS)

    Hong, Liang; Zhang, Rubing; Liu, Zheng

    2017-03-01

    Symmetric synthetic jet is used to stimulate a Mach number 0.9 and high temperature jet for the mixing enhancement. The movement of the piston-type synthetic jet is simulated by dynamic mesh based on URANS numerical methods. Jet flapping, streamwise vortex and geometric axis transformation are the jet mixing mechanism. However, the jet mixing enhancement effect depends on penetration area, which consists of penetration depth and spanwise. And the penetration area is determined by actuator nozzle diameter and blowing jet momentum. In addition, a 54% reduction in potential core length and maximum 45.8K decrease in potential temperature are achieved.

  16. Replacing the CCSDS Telecommand Protocol with the Next Generation Uplink (NGU)

    NASA Technical Reports Server (NTRS)

    Kazz, Greg J.; Greenberg, Ed; Burleigh, Scott C.

    2012-01-01

    The current CCSDS Telecommand (TC) Recommendations 1-3 have essentially been in use since the early 1960s. The purpose of this paper is to propose a successor protocol to TC. The current CCSDS recommendations can only accommodate telecommand rates up to approximately 1 mbit/s. However today's spacecraft are storehouses for software including software for Field Programmable Gate Arrays (FPGA) which are rapidly replacing unique hardware systems. Changes to flight software occasionally require uplinks to deliver very large volumes of data. In the opposite direction, high rate downlink missions that use acknowledged CCSDS File Delivery Protocol (CFDP)4 will increase the uplink data rate requirements. It is calculated that a 5 mbits/s downlink could saturate a 4 kbits/s uplink with CFDP downlink responses: negative acknowledgements (NAKs), FINISHs, End-of-File (EOF), Acknowledgements (ACKs). Moreover, it is anticipated that uplink rates of 10 to 20 mbits/s will be required to support manned missions. The current TC recommendations cannot meet these new demands. Specifically, they are very tightly coupled to the Bose-Chaudhuri-Hocquenghem (BCH) code in Ref. 2. This protocol requires that an uncorrectable BCH codeword delimit the TC frame and terminate the randomization process. This method greatly limits telecom performance since only the BCH code can support the protocol. More modern techniques such as the CCSDS Low Density Parity Check (LDPC)5 codes can provide a minimum performance gain of up to 6 times higher command data rates as long as sufficient power is available in the data. This paper will describe the proposed protocol format, trade-offs, and advantages offered, along with a discussion of how reliable communications takes place at higher nominal rates.

  17. Replacing the CCSDS Telecommand Protocol with the Next Generation Uplink (NGU)

    NASA Technical Reports Server (NTRS)

    Kazz, Greg J.; Greenberg, Ed; Burleigh, Scott C.

    2012-01-01

    The current CCSDS Telecommand (TC) Recommendations 1-3 have essentially been in use since the early 1960s. The purpose of this paper is to propose a successor protocol to TC. The current CCSDS recommendations can only accommodate telecommand rates up to approximately 1 mbit/s. However today's spacecraft are storehouses for software including software for Field Programmable Gate Arrays (FPGA) which are rapidly replacing unique hardware systems. Changes to flight software occasionally require uplinks to deliver very large volumes of data. In the opposite direction, high rate downlink missions that use acknowledged CCSDS File Delivery Protocol (CFDP)4 will increase the uplink data rate requirements. It is calculated that a 5 mbits/s downlink could saturate a 4 kbits/s uplink with CFDP downlink responses: negative acknowledgements (NAKs), FINISHs, End-of-File (EOF), Acknowledgements (ACKs). Moreover, it is anticipated that uplink rates of 10 to 20 mbits/s will be required to support manned missions. The current TC recommendations cannot meet these new demands. Specifically, they are very tightly coupled to the Bose-Chaudhuri-Hocquenghem (BCH) code in Ref. 2. This protocol requires that an uncorrectable BCH codeword delimit the TC frame and terminate the randomization process. This method greatly limits telecom performance since only the BCH code can support the protocol. More modern techniques such as the CCSDS Low Density Parity Check (LDPC)5 codes can provide a minimum performance gain of up to 6 times higher command data rates as long as sufficient power is available in the data. This paper will describe the proposed protocol format, trade-offs, and advantages offered, along with a discussion of how reliable communications takes place at higher nominal rates.

  18. Bilingual Dictionary of Mathematical Terms. English-Vietnamese = Tu-Dien Song Ngu Danh-Tu Toan. Anh-Viet.

    ERIC Educational Resources Information Center

    New York State Education Dept., Albany. Bureau of Bilingual Education.

    This bilingual dictionary was developed for use by Vietnamese junior high and high school students to assist them in their understanding of mathematical vocabulary and concepts in English. Terminology and definitions are provided in English, with Vietnamese translations directly below each entry. When possible, an illustration is included.…

  19. A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae).

    PubMed

    Deng, Yunyan; Wang, Xiuliang; Guo, Hui; Duan, Delin

    2014-01-01

    The full-length cDNA sequence of a trehalose-6-phosphate synthase gene from Saccharina japonica (designated as SjaTPS) (Accession: KC578568) was isolated based on homologous cloning and RACE-PCR. It was 4,127 bp, with 320 bp 5'-UTR, 21 bp 3'-UTR, and open reading frame (ORF) of 3,786 bp. The deduced 1,261 amino acids characterized with predicted molecular weight of 137.84 kDa and theoretical isoelectric point of 7.12. The SjaTPS had one N-terminal CBM20 (family 20 carbohydrate-binding module) domain, one TPS domain (trehalose-6-phosphate synthase) in the middle region and a single TPP (trehalose-6-phosphate phosphatase) domain near the C-terminus. Structural analysis suggested that the SjaTPS putatively functioned as trehalose-6-phosphate synthase, and might be related to laminaran metabolism in S. japonica. Homology analysis indicated that the SjaTPS shared 49-70 % similarities with the 13 known TPS sequences of other algae; only 55 % amino acid similarities were detected between SjaTPS and the previously reported TPS sequence of S. japonica (Accession: DQ666325). Phylogenetic analysis revealed close affinity between SjaTPS and TPS of brown alga Ectocarpus siliculosus (Accession: CBJ29609). Transcriptional analysis showed that desiccation greatly enhanced SjaTPS expression and the maximum appeared at 3 h, which was about 300-fold compared to that of the start, implied that SjaTPS was involved with drought adaption in kelp. In vitro expression of SjaTPS showed that one distinct band existed at ~115 kDa, and western blot detection proved that it was positive to the anti-His antibody with high specificity. Our results increased the knowledge of trehalose-6-phosphate synthase properties in S. japonica and also important for better understanding the role trehalose plays in kelp abiotic tolerance for adaption to the sublittoral habitats.

  20. Numerical Study of a Novel Piston-type Synthetic Jet Actuator with a Quick-return Characteristic

    NASA Astrophysics Data System (ADS)

    Liu, Zheng; Eriqitai; Hong, Liang

    2017-03-01

    A novel designed piston-type synthetic jet actuator has been developed, which has a quick-return characteristic. When moving to the Top Dead Centre, the piston in high speed route moves faster than the classical one. Compared with classical piston-type SJA, the pressure ratio of the cavity, jet velocity and the jet momentum of the novel SJA increase significantly, which greatly enhances the performance of the actuator. A parametric study has been carried out focusing on the affection in different actuation frequencies and duty cycle. Results show that the performance of the quick-return piston-type SJA is significantly improved.

  1. US Army Armor Reference Data in Three Volumes. Volume I. The Army Division.

    DTIC Science & Technology

    1981-01-01

    SP5 (Chauffeur) R I SP5 (Chauffeur) R 1 SP5 (Secy- Steno ) R I CSM (Comd SGT Major) R 2 SSG (Ent Aide) R I PFC (Dvr) R 1 SFC (Enl Aide) R 1 SP4 (Secy... Steno ) R 1 SP6 (Secy- Steno ) R I PFC (Secy- Steno ) R oe- IG SECTION - CHAPLAIN SECTION SJA SECTION I LTC linspector Gen) P 1 LTC (Div Chaplain) I MAJ...SJA) P I MAJ (Ch Trial Counsel) P I MSG (Chief Admin NCO) R I SP4 (Chapel Act Sp) (Dvr) R 2 CPT (Asst SJA) R 4 CPT (Defense Counsel) R 1 SP4 ( Steno

  2. 32 CFR 516.50 - Interference with mission.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Witnesses in Private Litigation § 516.50 Interference with mission. If the absence of a witness from duty will seriously interfere with the accomplishment of a military mission, the SJA or legal adviser...

  3. 32 CFR 516.50 - Interference with mission.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Witnesses in Private Litigation § 516.50 Interference with mission. If the absence of a witness from duty will seriously interfere with the accomplishment of a military mission, the SJA or legal adviser...

  4. 32 CFR 516.20 - Habeas Corpus.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... any civil officer having authority to apprehend offenders under the laws of the United States or of... the nature of a writ of habeas corpus, the SJA or legal adviser will immediately report the matter...

  5. Is a Simple Food-Diverting Operation the Solution for Type 2 Diabetes Treatment? Experimental Study in a Non-Obese Rat Model.

    PubMed

    Melissas, John; Peirasmakis, Drakos; Lamprou, Vasileios; Papadakis, John

    2016-05-01

    The feasibility of a side-to-side jejunoileal anastomosis (SJA) to control type 2 diabetes mellitus (T2DM) was studied in non-obese diabetic Goto-Kakizaki (GK) rats. Seventeen 14-week-old male GK rats were divided into three groups: SJA bypassing 60% of the small bowel length, sham-operated jejunoileal bypass (Sham group), and control animals. Rats were observed for 10 weeks after surgery. Fasting blood glucose (FBG) levels and oral glucose tolerance test (OGTT) were measured before and after the procedure. Animals with SJA exhibited normalization of FBG levels from the 1st and up to the 10th postoperative week when the experiment terminated. OGTT compared with sham-operated and control groups was also significantly better at 3 and 8 weeks postoperatively. A simple SJA, diverting the food and biliopancreatic secretion to the distal small bowel, was able to normalize both FBG levels and OGTT in a non-obese diabetic rat model.

  6. [Pathogen spectrum of non-gonorrhea urethritis].

    PubMed

    Schmidt, U; Hartmann, M; Pfützner, H

    1988-05-01

    By cultural investigations in 333 males with non-gonorrhoical urethritis (NGU) and 158 control probands it was demonstrated that Chlamydia trachomatis is the main germ of this disease also in the GDR with a frequency of 40% of the cases. A chlamydia-negative NGU was caused in 15% by Ureaplasma urealyticum in a number of greater than or equal to 10(4) germs/ml urine and in 5% by Trichomonas vaginalis, respectively. Candida albicans occurred significant more frequently in the patient group (21%) than in the control group (4%). Other optional pathogenic bacterias were the cause of NGU in single cases. The diagnosis and therapy of NGU should be considered this germ spectrum and the sexual partner also.

  7. The possible role of anaerobic bacteria in the aetiology of non-gonococcal urethritis in men.

    PubMed Central

    Hallén, A; Rydén, A C; Schwan, A; Wallin, J

    1977-01-01

    Men with non-gonococcal urethritis (NGU) were divided into two groups and treated with either lymecycline or tinidazole; anaerobic cultures were performed before and after treatment. Neither treatment affected the anaerobic flora. However, the men treated with lymecycline were relieved of symptoms and signs, while those treated with tinidazole were not. The anaerobic bacteria found are considered normal inhabitants of the urethral tract and without significance in the aetiology of NGU. PMID:606333

  8. The possible role of anaerobic bacteria in the aetiology of non-gonococcal urethritis in men.

    PubMed

    Hallén, A; Rydén, A C; Schwan, A; Wallin, J

    1977-12-01

    Men with non-gonococcal urethritis (NGU) were divided into two groups and treated with either lymecycline or tinidazole; anaerobic cultures were performed before and after treatment. Neither treatment affected the anaerobic flora. However, the men treated with lymecycline were relieved of symptoms and signs, while those treated with tinidazole were not. The anaerobic bacteria found are considered normal inhabitants of the urethral tract and without significance in the aetiology of NGU.

  9. Ureaplasma urealyticum is associated with nongonococcal urethritis among men with fewer lifetime sexual partners: a case-control study.

    PubMed

    Wetmore, Catherine M; Manhart, Lisa E; Lowens, M Sylvan; Golden, Matthew R; Jensen, Nicole L; Astete, Sabina G; Whittington, William L H; Totten, Patricia A

    2011-10-15

    Ureaplasmas have been inconsistently associated with nongonococcal urethritis (NGU). We evaluated the association of the newly differentiated species Ureaplasma urealyticum (UU) and Ureaplasma parvum (UP) with NGU using 2 separate control groups. Case patients were men who attended a sexually transmitted disease (STD) clinic in Seattle, Washington, during the period 2007-2009 with NGU (defined as visible urethral discharge and/or ≥5 polymorphonuclear neutrophils per high-powered field; n = 329). Control subjects were STD clinic attendees (n = 191) and emergency department (ED) attendees (n = 193) without NGU. Polymerase chain reaction assays detected UU and UP in ureaplasma culture-positive urine. Multivariable logistic regression was used to assess the associations of UU and UP with NGU. UU was only marginally associated with NGU in aggregate multivariable analyses, irrespective of control group (adjusted odds ratio [aOR](STD-control), 1.6 [95% confidence interval {CI}, 0.9-2.8]; aOR(ED-control), 1.7 [95% CI, 0.97-3.0]). This association was significantly stronger when analyses were restricted to men with fewer lifetime sex partners (<10 vaginal partners: aOR(STD-control), 2.9 [95% CI, 1.2-6.7]; aOR(ED-control), 3.2 [95% CI, 1.3-7.6]; <5 vaginal partners: aOR(STD-control), 6.2 [95% CI, 1.8-21.0]; aOR(ED-control), 5.2 [95% CI, 1.3-20.2]). UP was not positively associated with NGU overall or among subgroups. The absence of an association of UU with NGU among men with more lifetime sex partners suggests that adaptive immunity may attenuate the clinical manifestation of UU infection. Similar relationships were not observed with UP, which suggests that it is not a urethral pathogen.

  10. Differential association of ureaplasma species with non-gonococcal urethritis in heterosexual men

    PubMed Central

    Ondondo, Raphael O; Whittington, William L H; Astete, Sabina G; Totten, Patricia A

    2015-01-01

    Objective To assess the role of Ureaplasma urealyticum and Ureaplasma parvum in patients with non-gonococcal urethritis (NGU) using specimens from a previously reported study of NGU. Methods Species-specific PCR assays for U urealyticum and U parvum were used to detect these organisms in specimens from men enrolled in a case–control study based in a Seattle STD clinic in order to evaluate their association with NGU. Urethritis was defined by clinical examination and the presence of inflammation on Gram stained smear. Controls had normal examination findings and no evidence of inflammation on Gram stain smear or by the leucocyte esterase test. Results U urealyticum was detected in 26% (31/119) of cases and 16% (19/117) of controls, resulting in an association with NGU (adjusted odds ratio (aOR)=2.3, 95% CI 1.04 to 4.9) after adjusting for age, race, history of prior urethritis and other NGU pathogens (Chlamydia trachomatis, Mycoplasma genitalium). The association of U urealyticum and NGU was strongest in white men <28 years of age (OR=5.4, 95% CI 1.3 to 22.2). U parvum was detected in 14% (17/119) cases and 31% (36/117 controls) and thus was negatively associated with NGU (aOR=0.4, 95% CI 0.2 to 0.8). The prevalence of U urealyticum (16%) in controls was higher than that of C trachomatis (3.4%) or M genitalium (4.3%, p<0.05, each comparison). Conclusions Unlike U parvum, U urealyticum was associated with urethritis. The strong effect in younger white men and high rates in controls may suggest variability in virulence among U urealyticum strains or in host innate or acquired immunity. PMID:20460265

  11. Urethral inflammatory response to ureaplasma is significantly lower than to Mycoplasma genitalium and Chlamydia trachomatis.

    PubMed

    Moi, Harald; Reinton, Nils; Randjelovic, Ivana; Reponen, Elina J; Syvertsen, Line; Moghaddam, Amir

    2017-07-01

    A non-syndromic approach to treatment of people with non-gonococcal urethritis (NGU) requires identification of pathogens and understanding of the role of those pathogens in causing disease. The most commonly detected and isolated micro-organisms in the male urethral tract are bacteria belonging to the family of Mycoplasmataceae, in particular Ureaplasma urealyticum and Ureaplasma parvum. To better understand the role of these Ureaplasma species in NGU, we have performed a prospective analysis of male patients voluntarily attending a drop in STI clinic in Oslo. Of 362 male patients who were tested for NGU using microscopy of urethral smears, we found the following sexually transmissible micro-organisms: 16% Chlamydia trachomatis, 5% Mycoplasma genitalium, 14% U. urealyticum, 14% U. parvum and 5% Mycoplasma hominis. We found a high concordance in detecting in turn U. urealyticum and U. parvum using 16s rRNA gene and ureD gene as targets for nucleic acid amplification testing (NAAT). Whilst there was a strong association between microscopic signs of NGU and C. trachomatis infection, association of M. genitalium and U. urealyticum infections in turn were found only in patients with severe NGU (>30 polymorphonuclear leucocytes, PMNL/high powered fields, HPF). U. parvum was found to colonise a high percentage of patients with no or mild signs of NGU (0-9 PMNL/HPF). We conclude that urethral inflammatory response to ureaplasmas is less severe than to C. trachomatis and M. genitalium in most patients and that testing and treatment of ureaplasma-positive patients should only be considered when other STIs have been ruled out.

  12. 32 CFR 536.3 - Command and organizational relationships.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... also be the chief of the command claims service, however, the SJA may designate a field grade officer... offices (ACOs): (1) An office under the supervision of the senior judge advocate (JA) of each command or organization so designated by the Commander USARCS. The senior JA is the head of the ACO. (2) An office under...

  13. 32 CFR 842.121 - Referring a claim to the US Attorney.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Referring a claim to the US Attorney. 842.121... to the US Attorney. Only HQ USAF/JACC authorizes referral of a claim to the US Attorney. The base SJA...-case basis, HQ USAF/JACC will authorize referral of a case to the US Attorney by telephone....

  14. 32 CFR 842.121 - Referring a claim to the US Attorney.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Referring a claim to the US Attorney. 842.121... to the US Attorney. Only HQ USAF/JACC authorizes referral of a claim to the US Attorney. The base SJA...-case basis, HQ USAF/JACC will authorize referral of a case to the US Attorney by telephone....

  15. 32 CFR 842.121 - Referring a claim to the US Attorney.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Referring a claim to the US Attorney. 842.121... to the US Attorney. Only HQ USAF/JACC authorizes referral of a claim to the US Attorney. The base SJA...-case basis, HQ USAF/JACC will authorize referral of a case to the US Attorney by telephone....

  16. 32 CFR 842.121 - Referring a claim to the US Attorney.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Referring a claim to the US Attorney. 842.121... to the US Attorney. Only HQ USAF/JACC authorizes referral of a claim to the US Attorney. The base SJA...-case basis, HQ USAF/JACC will authorize referral of a case to the US Attorney by telephone....

  17. 32 CFR 842.121 - Referring a claim to the US Attorney.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Referring a claim to the US Attorney. 842.121... to the US Attorney. Only HQ USAF/JACC authorizes referral of a claim to the US Attorney. The base SJA...-case basis, HQ USAF/JACC will authorize referral of a case to the US Attorney by telephone....

  18. 32 CFR 516.50 - Interference with mission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Interference with mission. 516.50 Section 516.50... Witnesses in Private Litigation § 516.50 Interference with mission. If the absence of a witness from duty will seriously interfere with the accomplishment of a military mission, the SJA or legal adviser will...

  19. 32 CFR 842.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... in the regular procurement of services, supplies, equipment, or real estate. An oral demand made... of claims responsibility. The base Staff Judge Advocate's (SJA's) jurisdiction for claims. CONUS... responsibility to each military department. (f) HQ USAF/JACC. Claims and Tort Litigation Staff, Office of The...

  20. 32 CFR 516.51 - Response to subpoenas, orders, or requests for witnesses.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Witnesses Scope Litigation in Which the United States Has An Interest § 516.51 Response to subpoenas, orders... case and trial is imminent, the SJA or legal adviser should make informal arrangements to retain the witness in the command until trial. If this is not feasible, or if a satisfactory arrangement cannot be...

  1. 32 CFR 516.51 - Response to subpoenas, orders, or requests for witnesses.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Witnesses Scope Litigation in Which the United States Has An Interest § 516.51 Response to subpoenas, orders... case and trial is imminent, the SJA or legal adviser should make informal arrangements to retain the witness in the command until trial. If this is not feasible, or if a satisfactory arrangement cannot be...

  2. 32 CFR 516.51 - Response to subpoenas, orders, or requests for witnesses.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Witnesses Scope Litigation in Which the United States Has An Interest § 516.51 Response to subpoenas, orders... case and trial is imminent, the SJA or legal adviser should make informal arrangements to retain the witness in the command until trial. If this is not feasible, or if a satisfactory arrangement cannot be...

  3. 32 CFR 516.51 - Response to subpoenas, orders, or requests for witnesses.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Witnesses Scope Litigation in Which the United States Has An Interest § 516.51 Response to subpoenas, orders... case and trial is imminent, the SJA or legal adviser should make informal arrangements to retain the witness in the command until trial. If this is not feasible, or if a satisfactory arrangement cannot be...

  4. 32 CFR 516.51 - Response to subpoenas, orders, or requests for witnesses.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Witnesses Scope Litigation in Which the United States Has An Interest § 516.51 Response to subpoenas, orders... case and trial is imminent, the SJA or legal adviser should make informal arrangements to retain the witness in the command until trial. If this is not feasible, or if a satisfactory arrangement cannot be...

  5. 32 CFR 842.93 - Delegations of authority.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....93 Delegations of authority. (a) Settlement authority. (1) The following individuals have delegated... Staff. (2) The SJA of HQ 9AF (for CENTCOM), and the SJAs of PACAF and USAFE have delegated authority to... delegated authority to settle, compromise, suspend, or terminate action on claims for $15,000 or less and to...

  6. 32 CFR Appendix A to Part 584 - Reference

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... Army Reserve Components Personnel and Administration Center SJA Staff Judge Advocate SSN Social... USAFAC U.S. Army Finance and Accounting Center USAR U.S. Army Reserve VHA Variable Housing Allowance Section II—Terms Arrearage The total amount of money a soldier owes a family member for prior months in...

  7. 32 CFR Appendix A to Part 584 - Reference

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... Army Reserve Components Personnel and Administration Center SJA Staff Judge Advocate SSN Social... USAFAC U.S. Army Finance and Accounting Center USAR U.S. Army Reserve VHA Variable Housing Allowance Section II—Terms Arrearage The total amount of money a soldier owes a family member for prior months in...

  8. 32 CFR Appendix A to Part 584 - Reference

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Army Reserve Components Personnel and Administration Center SJA Staff Judge Advocate SSN Social... USAFAC U.S. Army Finance and Accounting Center USAR U.S. Army Reserve VHA Variable Housing Allowance Section II—Terms Arrearage The total amount of money a soldier owes a family member for prior months in...

  9. 32 CFR Appendix A to Part 584 - Reference

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... Army Reserve Components Personnel and Administration Center SJA Staff Judge Advocate SSN Social... USAFAC U.S. Army Finance and Accounting Center USAR U.S. Army Reserve VHA Variable Housing Allowance Section II—Terms Arrearage The total amount of money a soldier owes a family member for prior months in...

  10. 32 CFR Appendix A to Part 584 - Reference

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... Army Reserve Components Personnel and Administration Center SJA Staff Judge Advocate SSN Social... USAFAC U.S. Army Finance and Accounting Center USAR U.S. Army Reserve VHA Variable Housing Allowance Section II—Terms Arrearage The total amount of money a soldier owes a family member for prior months in...

  11. 32 CFR 513.1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... news media organizations to the unit, installation, or command public affairs officer for response. (6... received from news media organizations. (ii) Coordinate with the SJA before making any response. (e) Policy... include the soldier's full name, rank, and social security number (SSN). They should include the date and...

  12. 32 CFR 536.10 - Responsibilities and operations of claims processing offices.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Responsibilities and operations of claims processing offices. (a) Heads of CPOs. Heads of CPOs will: (1... Medical Center, may be designated by the SJA or head of the ACO for the installation on which the center... command claims service, or the head of an ACO may designate special CPOs within his or her command for...

  13. 32 CFR 536.10 - Responsibilities and operations of claims processing offices.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Responsibilities and operations of claims processing offices. (a) Heads of CPOs. Heads of CPOs will: (1... Medical Center, may be designated by the SJA or head of the ACO for the installation on which the center... command claims service, or the head of an ACO may designate special CPOs within his or her command for...

  14. 32 CFR 105.13 - Case management for Unrestricted Reports of sexual assault.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...; chaplain, legal representative, or SJA; installation personnel trained to do a safety assessment of current... on that trend data. (8) There will be a safety assessment capability. The CMG chair will identify installation personnel who have been trained and are able to perform a safety assessment of each sexual...

  15. The Delivery of Legal Services in USAREUR (United States Army, Europe): Lessons for all Staff Judge Advocates.

    DTIC Science & Technology

    1986-04-07

    unately they receive insufficient training in the managmenet principles and theories that should be applied by the SJA in supervising the delivery of...Chuck R. Pardue, "Ten Steps to a More Successful Legal Assistance Practice," DA Pam 27-50-154, October 1985. 20. An excellent introdution to time

  16. 32 CFR 516.46 - Records determined not to be releasable.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the records and the nonavailability of a claim of privilege. (ii) The clerk of the court is empowered... medical records would prejudice the cause of action, the matter will be reported to Litigation Division. (c) Referral to Litigation Division. If the SJA or legal adviser is not able to resolve a request for...

  17. 32 CFR 516.46 - Records determined not to be releasable.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the records and the nonavailability of a claim of privilege. (ii) The clerk of the court is empowered... medical records would prejudice the cause of action, the matter will be reported to Litigation Division. (c) Referral to Litigation Division. If the SJA or legal adviser is not able to resolve a request for...

  18. 32 CFR 516.23 - Litigation reports.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Litigation reports. 516.23 Section 516.23 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Reporting Legal Proceedings to HQDA § 516.23 Litigation reports. The SJA...

  19. Optimal Control of Airfoil Flow Separation using Fluidic Excitation

    NASA Astrophysics Data System (ADS)

    Shahrabi, Arireza F.

    This thesis deals with the control of flow separation around a symmetric airfoils with the aid of multiple synthetic jet actuators (SJAs). CFD simulation methods have been implemented to uncover the flow separation regimes and associated properties such as frequencies and momentum ratio. In the first part of the study, the SJA was studied thoroughly. Large Eddy Simulations (LES) were performed for one individual cavity; the time history of SJA of the outlet velocity profile and the net momentum imparted to the flow were analyzed. The studied SJA is asymmetrical and operates with the aid of a piezoelectric (PZT) ceramic circular plate actuator. A three-dimensional mesh for the computational domain of the SJA and the surrounding volume was developed and was used to evaluate the details of the airflow conditions inside the SJA as well as at the outlet. The vibration of the PZT ceramic actuator was used as a boundary condition in the computational model to drive the SJA. Particular attention was given to developing a predictive model of the SJA outlet velocity. Results showed that the SJA velocity output is correlated to the PZT ceramic plate vibration, especially for the first frequency mode. SJAs are a particular class of zero net mass flux (ZNMF) fluidic devices with net imparted momentum to the flow. The net momentum imparted to the flow in the separated region is such that positive enhancement during AFC operations is achieved. Flows around the NACA 0015 airfoil were simulated for a range of operating conditions. Attention was given to the active open and closed loop control solutions for an airfoil with SJA at different angles of attack and flap angles. A large number of simulations using RANS & LES models were performed to study the effects of the momentum ratio (Cμ) in the range of 0 to 11% and of the non-dimensional frequency, F+, in the range of 0 to 2 for the control of flow separation at a practical angle of attack and flap angle. The optimum value of C

  20. If Your Child Learns in Two Languages: A Parent's Guide for Improving Educational Opportunities for Children Acquiring English as a Second Language = Si su nino aprende en dos idiomes: Una guia para que las familias sepan como mejorar las oportunidades educativas de los ninos que adquieren el ingles como segunda lengua = Neu lon Ban Hoc Bang Hai Thu Tieng: Chi-nam cua phu-huynh de cai tien co hoi hoc van cua con em dang hoc Anh van nhu sinh ngu thu hai.

    ERIC Educational Resources Information Center

    Zelasko, Nancy; Antunez, Beth

    This guide, in English, Spanish, and Vietnamese, aims to inform parents of students who have learned or are learning English as a Second Language about appropriate approaches for educating children so that they can work with schools to ensure a high quality education for their children. The emphasis is on explaining the laws, schools, and research…

  1. Ureaplasma urealyticum and Mycoplasma hominis in chlamydial and non-chlamydial nongonococcal urethritis.

    PubMed Central

    Taylor-Robinson, D; Evans, R T; Coufalik, E D; Prentice, M J; Munday, P E; Csonka, G W; Oates, J K

    1979-01-01

    Urethral specimens from 726 patients with nongonococcal urethritis (NGU) were examined for Chlamydia trachomatis, Ureaplasma urealyticum, and Mycoplasma hominis. Chlamydiae were isolated from 35.9% of ureaplasma-positive patients and from 36.5% of ureaplasma-negative patients. Ureaplasmas were isolated from 52.5% of chlamydia-positive patients and from 53.1% of chlamydia-negative patients, an observation which contrasts with that of some workers who have suggested that ureaplasmas are significantly associated with chlamydia-negative NGU. Furthermore, the numbers of ureaplasmas isolated from patients who did or did not harbour chlamydiae were not significantly different nor was there a particular association of ureaplasmas with chlamydia-negative NGU in patients experiencing their first episode of disease. In addition, M. hominis was not isolated more frequently from those from whom chlamydiae were or were not isolated. The only significant associations were the isolation of M. hominis from patients who were ureaplasma-positive and of ureaplasmas from those who were M. hominis-positive. These findings do not necessarily mitigate against ureaplasmas being responsible for some cases of chlamydia-negative NGU. PMID:427513

  2. Sexually Transmitted Diseases - Multiple Languages: MedlinePlus

    MedlinePlus

    ... sharing features on this page, please enable JavaScript. Amharic (amarunya) Arabic (العربية) Bosnian (Bosanski) Chinese - Simplified (简体中文) ... Русский) Somali (af Soomaali) Spanish (español) Tagalog (Tagalog) Amharic (amarunya) Nongonococcal Urethritis (NGU) English amarunya (Amharic) PDF ...

  3. Comparative performance of traps in catching tsetse flies (Diptera: Glossinidae) in Tanzania.

    PubMed

    Malele, Imna I; Ouma, Johnson O; Nyingilili, Hamisi S; Kitwika, Winston A; Malulu, Deusdedit J; Magwisha, Henry B; Kweka, Eliningeya J

    2016-06-23

    This study was conducted to determine the efficiency of different tsetse traps in 28 sites across Tanzania. The traps used were biconical, H, NGU, NZI, pyramidal, S3, mobile, and sticky panels. Stationary traps were deployed at a distance of 200 m apart and examined 72 h after deployment. The results showed that 117 (52.2%) out of the 224 traps deployed captured at least one Glossina species. A total of five Glossina species were captured, namely Glossina brevipalpis, Glossina pallidipes, Glossina swynnertoni, Glossina morsitans, and Glossina fuscipes martinii. Biconical traps caught tsetse flies in 27 sites, pyramidal in 26, sticky panel in 20, mobile in 19, S3 in 15, NGU in 7, H in 2 and NZI in 1. A total of 21 107 tsetse flies were trapped, with the most abundant species being G. swynnertoni (55.9%), followed by G. pallidipes (31.1%), G. fuscipes martinii (6.9%) and G. morsitans (6.0%). The least caught was G. brevipalpis (0.2%). The highest number of flies were caught by NGU traps (32.5%), followed by sticky panel (16%), mobile (15.4%), pyramidal (13.0%), biconical (11.3%) and S3 (10.2%). NZI traps managed to catch 0.9% of the total flies and H traps 0.7%. From this study, it can be concluded that the most efficient trap was NGU, followed by sticky panel and mobile, in that order. Therefore, for tsetse fly control programmes, NGU traps could be the better choice. Conversely, of the stationary traps, pyramidal and biconical traps captured tsetse flies in the majority of sites, covering all three ecosystems better than any other traps; therefore, they would be suitable for scouting for tsetse infestation in any given area, thus sparing the costs of making traps for each specific Glossina species.

  4. Design & Development of a High Mass Flow Piston Synthetic Jet Actuator

    NASA Astrophysics Data System (ADS)

    Ashraf, Hamad Muhammad; Murugan Illikkal, Karthika; D'souza, Francis; Alsayed Mahmood, Mohamed; Mahmud Mostafa, Suhail; Kim, Young Hwan

    2015-05-01

    The idea of having a device that is capable of working in a systematic process allowing control of the boundary layer by means of operated on high-frequency, small-scale, and low energy actuators has caught the interest of the aerodynamicist community. With an eye on the available data and potential flow control advantages, our research team set out to manufacture a compact SJA (Synthetic Jet Actuator) of its own, which would be capable of being installed inside an airfoil. It consists of components such as a single piston cylinder, with variable exit geometry along with the control system that has an electrical actuator which can be regulated in order for it to be capable of producing various operating frequencies. This paper consists of a study into the design of a single piston device SJA and will present all significant data both theoretical and computational regarding its design and performance.

  5. Expulsion of Hymenolepis nana from mice with congenital deficiencies of IgE production or of mast cell development.

    PubMed

    Watanabe, N; Nawa, Y; Okamoto, K; Kobayashi, A

    1994-03-01

    The roles of IgE and mast cells on expulsion of adult Hymenolepis nana from the intestine were examined in mice. IgE-dependency was determined by comparing congenitally IgE-deficient SJA/9 and IgE-producing SJL/J mice infected with 50 H. nana eggs. Anti-H. nana IgE antibody was detected at three weeks post infection (p.i.) in SJL but not in SJA mice. The number of adult worms in the intestines of SJA and of SJL mice were similar at two weeks, but significantly more were found in SJA mice at three weeks p.i. Treatment of mice with anti-epsilon antibody also resulted in an increased worm burden at three weeks, suggesting participation of IgE in expulsion of H. nana. Intestinal mastocytosis was induced by infection regardless of the IgE status of the mice. Mast cell-dependency was tested in mast cell-deficient W/Wv and in normal littermate +/+ mice infected with 100 H. nana eggs. Anti-H. nana antibody was detected in both groups of mice at three weeks p.i. Worm expulsion seemed to be mast cell dependent because expulsion was less complete in W/Wv mice at three weeks p.i. Peripheral blood eosinophilia was comparable at three weeks p.i. in both IgE and mast cell sufficient and deficient mice. These results suggest that IgE and mast cells participate in the expulsion of H. nana adults from intestine in mice.

  6. Inactivation: A Blueprint for a Division

    DTIC Science & Technology

    1991-04-05

    installations to address any proposed actions potentially effecting the quality of the environment . The format of the mandated environmental assessment...authority as the divison incrementally stands down. Finally, the SJA will fulfill a major responsibility in the preparation of the Environmental ...Assessment (EA) as discussed in the following section. ENBI R OWENTAL ISSUES THE NATIONAL ENVIRONMENTAL POLICY ACT OF 1969 (NEPA) requires all federal

  7. Staff Judge Advocate Handbook. Revision

    DTIC Science & Technology

    1990-04-01

    working cooperation for keeping each other informed and ensuring service record entries are made, pay stops and starts as appropriate, division... Work closely with TC 1. Serve accused when he is aboard 2. Supply sufficient copies of charge sheet, etc. 3. Ensure that service record entries are...accurate 4. Make DC work through TC SJA-7 r. Accused works for- command, not for DC 1. Use check-in/check-out chits for visits to DC, and retain them in

  8. A Simple Food-Diverting Operation for Type 2 Diabetes Treatment. Preliminary Results in Humans with BMI 28-32 kg/m(2).

    PubMed

    Melissas, John; ErenTaskin, Halit; Peirasmakis, Drakos; Dimitriadis, Efstathios; Papadakis, Marios; Zengin, Seniyye Ulgen; Yumuk, Volkan; Taskin, Mustafa

    2017-01-01

    The feasibility of a simple side-to-side jejunoileal anastomosis (SJA) in non-morbidly obese individuals to control type 2 diabetes mellitus (T2DM) was studied in six diabetic patients with BMI 28-32. This novel procedure was performed in two Academic Centers and preliminary data is presented. SJA bypassing a significant part of the small bowel and diverting food and biliopancretic secretions to the distal ileum was performed laparoscopically in six diabetic patients with BMI 28-32 kg/m(2). SJA was performed at a distance of 100 cm from the Treitz ligament and 150 cm from the ileocecal valve, thus a total bowel alimentary and biliopancreatic loop length of 2.5 m was created. Three of the patients (two female, one male) experienced complete remission of diabetes from the immediate postoperative period and up to 3 years after the procedure. Two other patients remained medication-free after the operation experiencing partial diabetes remission, and the third patient significantly reduced his daily insulin requirements immediately after surgery. There were no early or late postoperative complications. A simple SJA, diverting the food and biliopancreatic secretion to the distal small bowel, was able to control T2DM in all patients offering total or partial remission of the disease soon after the procedure. Those preliminary data and the simplicity of the operation are encouraging and promising. However, further studies are in progress in both Institutions to reveal the possible mechanism of diabetes control and investigate the effect of this operation on larger number of patients, with longer follow-up period.

  9. Solubility-insolubility interconversion of sophoragrin, a mannose/glucose-specific lectin in Sophora japonica (Japanese pagoda tree) bark, regulated by the sugar-specific interaction.

    PubMed

    Ueda, Haruko; Fukushima, Hisako; Hatanaka, Yasumaru; Ogawa, Haruko

    2004-09-15

    Sophoragrin, a mannose/glucose-specific lectin in Sophora japonica (Japanese pagoda tree) bark, was the first lectin found to show self-aggregation that is dependent on the sugar concentration accompanying the interconversion between solubility and insolubility [Ueno, Ogawa, Matsumoto and Seno (1991) J. Biol. Chem. 266, 3146-3153]. The interconversion is regulated by the concentrations of Ca(2+) and specific sugars: mannose, glucose or sucrose. The specific glycotopes for sophoragrin were found in the sophoragrin subunit and an endogenous galactose-specific lectin, B-SJA-I (bark S. japonica agglutinin I), and the lectin subunit that binds to the glycotope was identified by photoaffinity glycan probes. Remarkably, the insoluble polymer of sophoragrin is dissociated by interaction with B-SJA-I into various soluble complexes. Based on these results, self-aggregation of sophoragrin was shown to be a unique homopolymerization due to the sugar-specific interaction. An immunostaining study indicated that sophoragrin localizes mainly in vacuoles of parenchymal cells coincidently with B-SJA-I. These results indicate that sophoragrin can sequester endogenous glycoprotein ligands via sugar-specific interactions, thus providing new insights into the occurrence and significance of the intravacuolar interaction shown by a legume lectin.

  10. Inbred strains of zebrafish exhibit variation in growth performance and myostatin expression following fasting.

    PubMed

    Meyer, Ben M; Froehlich, Jacob M; Galt, Nicholas J; Biga, Peggy R

    2013-01-01

    Although the zebrafish (Danio rerio) has been widely utilized as a model organism for several decades, there is little information available on physiological variation underlying genetic variation among the most commonly used inbred strains. This study evaluated growth performance using physiological and molecular markers of growth in response to fasting in six commonly used zebrafish strains [AB, TU, TL, SJA, WIK, and petstore (PET) zebrafish]. Fasting resulted in a standard decrease in whole blood glucose levels, a typical vertebrate glucose metabolism pattern, in AB, PET, TL, and TU zebrafish strains. Alternatively, fasting did not affect glucose levels in SJA and WIK zebrafish strains. Similarly, fasting had no effect on myostatin mRNA levels in AB, PET, TU, and WIK zebrafish strains, but decreased myostatin-1 and -2 mRNA levels in SJA zebrafish. Consistent with previous work, fasting increased myostatin-2 mRNA levels in TL zebrafish. These data demonstrate that variation is present in growth performance between commonly used inbred strains of zebrafish. These data can help future research endeavors by highlighting the attributes of each strain with regard to growth performance so that the most fitting strain may be utilized. Copyright © 2012 Elsevier Inc. All rights reserved.

  11. Inbred strains of zebrafish exhibit variation in growth performance and myostatin expression following fasting

    PubMed Central

    Meyer, Ben M.; Froehlich, Jacob M.; Galt, Nicholas J.; Biga, Peggy R.

    2012-01-01

    Although the zebrafish (Danio rerio) has been widely utilized as a model organism for several decades, there is little information available on physiological variation underlying genetic variation among the most commonly used inbred strains. This study evaluated growth performance using physiological and molecular markers of growth in response to fasting in six commonly used zebrafish strains [AB, TU, TL, SJA, WIK, and petstore (PET) zebrafish]. Fasting resulted in a standard decrease in whole blood glucose levels, typical vertebrate glucose metabolism pattern, in AB, PET, TL, and TU zebrafish strains. Alternatively, fasting did not affect glucose levels in SJA and WIK zebrafish strains. Similarly, fasting had no effect on myostatin mRNA levels in AB, PET, TU, and WIK zebrafish strains, but decreased myostatin-1 and -2 mRNA levels in SJA zebrafish. Consistent with previous work, fasting increased myostatin-2 mRNA levels in TL zebrafish. These data demonstrate that variation is present in growth performance between commonly used inbred strains of zebrafish. These data can help future research endeavors by highlighting the attributes of each strain with regard to growth performance so that the most fitting strain may be utilized. PMID:23047051

  12. Evaluation of Johnson-Cook model constants for aluminum based particulate metal matrix composites

    NASA Astrophysics Data System (ADS)

    Hilfi, H.; Brar, N. S.

    1996-05-01

    High strain rate and high temperature response of three types of aluminum based particulate metal matrix ceramic composites is investigated by performing split Hopkinson pressure bar (SHPB) experiments. The composites are: NGP-2014 (15% SiC), NGT-6061 (15% SiC), and NGU-6061 (15% Al2O3), in which all the reinforcement materials are percentage by volume. Johnson-Cook constitutive model constants are evaluated from the high strain rate/high temperature data and implemented in a two dimensional finite element computer code (EPIC-2D) to simulate the penetration of an ogive nose tungsten projectile (23 grams) at a velocity 1.17 km/sec into the base 6061-T6 aluminum alloy and the composite NGU-6061. The simulated penetrations in the composite and in 6061-T6 aluminum agree with in 2%, in both materials, with the measured values.

  13. Managing Geological Profiles in Databases for 3D Visualisation

    NASA Astrophysics Data System (ADS)

    Jarna, A.; Grøtan, B. O.; Henderson, I. H. C.; Iversen, S.; Khloussy, E.; Nordahl, B.; Rindstad, B. I.

    2016-10-01

    Geology and all geological structures are three-dimensional in space. GIS and databases are common tools used by geologists to interpret and communicate geological data. The NGU (Geological Survey of Norway) is the national institution for the study of bedrock, mineral resources, surficial deposits and groundwater and marine geology. 3D geology is usually described by geological profiles, or vertical sections through a map, where you can look at the rock structure below the surface. The goal is to gradually expand the usability of existing and new geological profiles to make them more available in the retail applications as well as build easier entry and registration of profiles. The project target is to develop the methodology for acquisition of data, modification and use of data and its further presentation on the web by creating a user-interface directly linked to NGU's webpage. This will allow users to visualise profiles in a 3D model.

  14. Conference Proceedings of The Human-Electronic Crew: Can They Work Together? Held in Ingolstadt, FRG on 19-22 September 1988

    DTIC Science & Technology

    1989-07-01

    these constraints, there is also the problem of memory volume: Al applications are generally ’greedy’ In memory requirements and all use a ’ garbage ...rAau Om nom TOM sm06- iMOM 152 1.2 Al TECNOLOGY - UNRESOLVED OVA$ -*M i swam .. MGM * I mMm a a~~M amuji hi "num.m W u L WW " NOu-M I ssw nGU"m on"a

  15. Nonlinear Theory for Partially Cavitating Cascade Flows.

    DTIC Science & Technology

    1978-09-01

    velocity normalized by U2 = £n(q/U2)~ potential function stream function w hodograph variable = e+iT) vi Subscripts: 1 and 2 denotes quantities...a hodograph variable ~ = q e~~ 8 = U2e~~~ (6) or (7) ~ L n(g/U 2 ) (8) where denotes the ve locity at downstream inf ini ty , we can express the

  16. The DOD Problem in the Mechanical Translation Field

    DTIC Science & Technology

    1961-01-01

    an4 disse~tion . in translated.for.m); b.. the quality of translationj c. the la.ngu&ges .to be traslated ; aDd d. the cost of translation...exteugb to warr&.l!ll.t aec®pt~e · ot the IEM proposal for a NatiolMJ. Traslation Ce;1mter {nor that the Air :!force should. support., alort\\e

  17. Phylogeny-Based Rapid Identification of Mycoplasmas and Ureaplasmas from Urethritis Patients

    PubMed Central

    Yoshida, Takashi; Maeda, Shin-Ichi; Deguchi, Takashi; Ishiko, Hiroaki

    2002-01-01

    Some strains of mycoplasmas and ureaplasmas (family Mycoplasmataceae) are associated with nongonococcal urethritis (NGU) or other genitourinary infections. We have developed a rapid and reliable method of identifying the presence and prevalence of mycoplasmas and ureaplasmas in men with NGU. This method is based on the amplification of a part of the 16S rRNA gene by PCR and phylogenetic analysis. A portion of the 16S rRNA gene from 15 prototype strains was amplified with a set of common primers, and their nucleotides were sequenced. The nucleotide sequence of the V4 and V5 regions was analyzed by the neighbor-joining method. The 15 prototype strains were grouped into three distinct clusters, allowing us to clearly segregate the strains into distinct lineages. To determine the prevalence of these pathogens among patients with NGU, this protocol was tested with 148 urine samples. Amplifications were observed for 42 samples, and their nucleotide sequences were analyzed along with those of the 15 prototype strains. The phylogenetic tree thus constructed indicated that 15 of the 42 formed a cluster with Mycoplasma genitalium. Among the remaining specimens, 2 formed a cluster with Mycoplasma hominis, 19 with Ureaplasma urealyticum, and 5 with Ureaplasma parvum; the remaining sample contained both M. genitalium and U. urealyticum. This phylogeny-based identification of mycoplasmas and ureaplasmas provides not only a powerful tool for rapid diagnosis but also the basis for etiological studies of these pathogens. PMID:11773101

  18. The enigma of non-gonococcal urethritis: role for Bacteroides ureolyticus.

    PubMed Central

    Hawkins, D A; Fontaine, E A; Thomas, B J; Boustouller, Y L; Taylor-Robinson, D

    1988-01-01

    Although up to about half the cases of acute non-gonococcal urethritis (NGU) are caused by Chlamydia trachomatis organisms (chlamydiae) and a smaller, ill-defined, proportion probably by Ureaplasma urealyticum organisms (ureaplasmas), the aetiology of all cases is not understood. Clarification of the role of the anaerobe, Bacteroides ureolyticus, was sought in the current study. Seventy five chlamydia negative patients with NGU were treated on a double blind placebo controlled basis with metronidazole. After seven days more of the 35 patients given this drug tended to improve clinically than the 40 given the placebo, but the difference was not significant. Of 23 chlamydia negative but anaerobe positive men, however, 78% (7/9) receiving metronidazole responded clinically, but only 7% (1/14) receiving placebo responded (p less than 0.001). Furthermore, whereas 78% of the anaerobe positive men given metronidazole recovered, only 23% (6/26) of the anaerobe negative men did so (p less than 0.02). No further evidence for the role of ureaplasmas in the aetiology of NGU was obtained, but the data suggest that B ureolyticus organisms, and perhaps other anaerobes, have an important role in a small proportion of cases and that the beneficial effects of metronidazole given on an empirical basis will be confined to anaerobe positive urethritis. PMID:3278969

  19. Features of cellular effects of combined exposure to ionizing radiation and copper ions.

    PubMed

    Hapieienko, D D

    2013-01-01

    Postijne zrostannja masshtabiv vykorystannja himichnyh rechovyn ta dzherel ionizujuchogo vyprominjuvannja v riznyh galuzjah promyslovosti, medycyni, nauci zbil'shuje i'h vplyv na vsi komponenty pryrodnogo seredovyshha. Tozh zrostaje jmovirnist' odnochasnogo vplyvu radiacijnogo ta himichnogo faktoriv na biologichni ob'jekty, a v zv’jazku z cym pytannja osoblyvostej kombinovanoi' dii' riznyh za svojeju pryrodoju faktoriv staje vse bil'sh aktual'nym. Metoju roboty stalo doslidzhennja osoblyvostej okremogo ta kombinovanogo vplyvu ionizujuchogo vyprominjuvannja ta solej midi na zhyttjezdatnist' klityn in vitro. Materialy i metody. Doslidzhennja vykonani na pereshhepljuvanij kul'turi klityn linii' L929. Acetat midi (Cu(CH3COOH)2) dodavaly do kul'tury klityn cherez 1 godynu pislja i'h oprominennja. Oprominennja klityn provodyly cherez 24 godyny pislja posadky. Proliferatyvnu aktyvnist' klityn ocinjuvaly za kinetykoju rostu. Rezul'taty doslidzhen' pokazaly, shho cytotoksychnist' ioniv midi projavljajet'sja pry koncentracii' 1 mkmol'/l. Kombinovana dija ionizujuchogo vyprominjuvannja v subletal'nyh dozah ta ioniv midi v koncentracijah 0,001 ta 1 mkmol'/l pryzvela do zbil'shennja kil'kosti atypovyh polikariocytiv. Vysnovky. Pry inkubacii' klityn z ionamy midi v riznyh koncentracijah sposterigajet'sja jak aktyvacija, tak i ingibuvannja proliferatyvnoi' ta mitotychnoi' aktyvnosti klityn. Vyzhyvannja klityn ta mitotychna aktyvnist' pry oprominenni zmenshujut'sja z pidvyshhennjam dozy, a indeks polikariocytiv zrostaje. Pry kombinovanomu vplyvi ionizujuchogo vyprominjuvannja ta ioniv midi na klityny in vitro sposterigaly zminy morfofunkcional'nyh vlastyvostej klityn, jaki prjamo proporcijno ne zalezhaly ni vid dozy oprominennja, ni vid koncentracii' ioniv midi.

  20. Development of high-power, compact synthetic jet actuators for flow separation control

    NASA Astrophysics Data System (ADS)

    Gilarranz Runge, Jose Luis

    This work presents the development of high-power, compact synthetic jet actuators (SJA) for flow separation control. The developed actuator is compact enough to fit in the interior of a NACA0015 profiled wing with a chord of 0.375 in. Test bench experiments showed that the multi-piston actuator array was capable of producing exit velocities of up to 90 m/s for an actuator frequency of 130 Hz. The actuator was placed in a NACA 0015 wing and tested in a wind tunnel. An experimental investigation into the effects of a synthetic jet actuator on the performance of the wing is described. Emphasis is placed on the capabilities of the actuator to control the separation of the flow over the wing at high angles of attack. The investigation included the use of force balance measurements, on-surface flow visualization with oil and tufts, off-surface flow visualizations with smoke, surface pressure distribution measurements and wake surveys. In addition to flow separation control data, results corresponding to hot wire measurements at the exit of the slot, are also presented and are used for the characterization of the flowfield generated by the synthetic jet actuators. Most of the tests were performed at a freestream velocity of 35 m/s, corresponding to a Reynolds number of 8.96 x 105. The angle of attack was varied from -2.0 to 29 degrees. For the tests presented here, at angles of attack lower than 10 degrees the actuator tends to increase the lift curve slope as the actuation frequency is increased. At higher angles of attack, the SJA extends the range of angle of attack for which the wing may be operated without stalling. The use of the actuator causes an 80% increase in the value of maximum lift coefficient, and the angle at which stall occurs is increased from 12 to 18 degrees (for the Reynolds number range of the test). The drag on the wing is decreased as a consequence of SJA actuation. This was verified with the force balance measurements and by analysis of the wake

  1. Advanced Modified High Performance Synthetic Jet Actuator with Curved Chamber

    NASA Technical Reports Server (NTRS)

    Xu, Tian-Bing (Inventor); Su, Ji (Inventor); Jiang, Xiaoning (Inventor)

    2014-01-01

    The advanced modified high performance synthetic jet actuator with optimized curvature shape chamber (ASJA-M) is a synthetic jet actuator (SJA) with a lower volume reservoir or chamber. A curved chamber is used, instead of the conventional cylinder chamber, to reduce the dead volume of the jet chamber and increase the efficiency of the synthetic jet actuator. The shape of the curvature corresponds to the maximum displacement (deformation) profile of the electroactive diaphragm. The jet velocity and mass flow rate for the ASJA-M will be several times higher than conventional piezoelectric actuators.

  2. STD/HIV control in Malawi and the search for affordable and effective urethritis therapy: a first field evaluation.

    PubMed Central

    Lule, G; Behets, F M; Hoffman, I F; Dallabetta, G; Hamilton, H A; Moeng, S; Liomba, G; Cohen, M S

    1994-01-01

    OBJECTIVES--To evaluate gonococcal (GU) and nongonococcal urethritis (NGU), chlamydia antigen, and serostatus for syphilis and human immunodeficiency virus (HIV) among males attending a Malawian STD clinic with complaints of urethral discharge and/or dysuria. To collect demographic and behavioural data and to determine the effectiveness of five treatments for urethritis. METHODS--Urethritis was diagnosed using microscopy and culture for Neisseria gonorrhoeae. Sera were screened with rapid plasma reagin (RPR) and if reactive, with microhaemagglutination for Treponema pallidum (MHA-TP). HIV antibodies and chlamydia antigen were detected using enzyme immunoassay. Patients were randomised for treatment, cure was assessed 8-10 days later. RESULTS--At enrolment, GU was diagnosed in 415 (80.3%) and NGU in 59 (11.2%) of 517 males. Chlamydia antigen was found in 26 (5.2%) of 497 specimens tested. Syphilis seropositivity rate (RPR and MHA-TP reactive) was 10.7%. Overall HIV seroprevalence was 44.2%; 71.7% of men with reactive syphilis serology were HIV(+) compared with 40.9% of syphilis seronegatives (OR: 3.6, p < 0.001). Trimethoprim 320 mg/sulphamethoxazole 1600 mg by mouth for 2 days (TMPSMX), or the combination of amoxicillin 3 gm, probenicid 1 gm, and clavulanate 125 mg by mouth once (APC), failed to cure gonorrhoea effectively. Amoxicillin 3 gm, probenicid 1 gm, and clavulanate 125 mg, by mouth once with doxycycline 100 mg BID for 7 days (APC-D), gentamicin 240 mg IM once (GENT), ciprofloxacin 250 mg by mouth once (CIPRO) cured 92.9% to 95% of gonorrhoea. APC-D treatment did not generate less NGU at follow-up. HIV serostatus did not affect cure of urethritis. CONCLUSION--All patients presenting with urethritis should be treated syndromically using a simple algorithm and screened for syphilis seroreactivity for appropriate treatment and counselling. PMID:7705854

  3. Efficacy of standard therapies against Ureaplasma species and persistence among men with non-gonococcal urethritis enrolled in a randomized controlled trial

    PubMed Central

    Khosropour, Christine M.; Manhart, Lisa E.; Gillespie, Catherine W.; Lowens, M. Sylvan; Golden, Matthew R.; Jensen, Nicole L.; Kenny, George E.; Totten, Patricia A.

    2015-01-01

    Objective U. urealyticum biovar 2 (UU-2) but not U. parvum (UP) has been associated with non-gonococcal urethritis (NGU), but little is known about species-specific responses to standard therapies. We examined species-specific treatment outcomes and followed men with treatment failure for 9 weeks. Methods From May 2007-July 2011, men aged ≥16 attending an STD clinic in Seattle, Washington with NGU (urethral discharge or urethral symptoms plus ≥5 PMNs/HPF) were enrolled in a double-blind, randomized trial. Participants received active azithromycin (1g) + placebo doxycycline or active doxycycline (100mg bid × 7d) + placebo azithromycin. Ureaplasmas were detected in culture followed by species-specific PCR. Outcomes were assessed at 3, 6, and 9 weeks. At 3 weeks, men with persistent Ureaplasmas received “reverse therapy” (e.g., active doxycycline if they first received active azithromycin). At 6 weeks, persistently-positive men received moxifloxacin (400mg × 7d). Results Of 490 men, 107 (22%) and 60 (12%) were infected with UU-2 and UP, respectively, and returned at 3 weeks. Persistent infection was similar for UU-2-infected men initially treated with azithromycin or doxycycline (25% vs. 31%, P=0.53), but differed somewhat for men with UP (45% vs. 24%; P=0.11). At 6 weeks, 57% of UU-2-infected and 63% of UP-infected men who received both drugs had persistent infection. Failure after moxifloxacin occurred in 30% and 36%, respectively. Persistent detection of UU-2 or UP was not associated with signs/symptoms of NGU. Conclusion Persistent infection after treatment with doxycycline, azithromycin, and moxifloxacin was common for UU and UP, but not associated with persistent urethritis. PMID:25616607

  4. A Model for Translating MAC (Military Airlift Command) Flying Hours into Airlift Capability

    DTIC Science & Technology

    1988-09-01

    DOVER (DOV) to: From PATRICK (COF) to: Germany (GER) Africa (AFR) Mediterranean (MED) Middle East (M/E) From MCGUIRE (WRI) to: From NORFOLK (NGU) to...portion of C-5 hours. In the Atlantic region (Table 4.4), the MAI WRI-MED ( McGuire to the Mediterranean) has the largest portion of C-141 hours while DOV...Thumrait 1 x 20 = 20 04V7 Norfolk - Rota 4 x 20 80 Total Capability in Tons 1700 438 MAW 0707 McGuire - FSS 30 x 20 = 600 0759 McGuire - MED 4 x 20

  5. A Random Finite Set Approach to Space Junk Tracking and Identification

    DTIC Science & Technology

    2014-09-03

    Final 3. DATES COVERED (From - To) 31 Jan 13 – 29 Apr 14 4. TITLE AND SUBTITLE A Random Finite Set Approach to Space Junk Tracking and...01-2013 to 29-04-2014 4. TITLE AND SUBTITLE A Random Finite Set Approach to Space Junk Tracking and Identification 5a. CONTRACT NUMBER FA2386-13...Prescribed by ANSI Std Z39-18 A Random Finite Set Approach to Space Junk Tracking and Indentification Ba-Ngu Vo1, Ba-Tuong Vo1, 1Department of

  6. The Toxicity of Nitroguanidine and Photolyzed Nitroguandine to Freshwater Aquatic Organisms

    DTIC Science & Technology

    1985-03-01

    Paratanytarsus dissimlils), and aquatic worms ( Lumbriculus variegatus ). The. IN) tWS UCLASSIFIED SEURIY4T CLAISIPICAtIOW OF TNIS PAGE (1160 Date U40*4. I. II...2868 0 ( Lumbriculus variegatue ) 1785 0 (1.49 0 a. The high, low, and control NGu concentrations are listed for each test. The top concentration tested...Bailey, H.C. and D.H.W. Liu. 1980. Lumbriculus variegatus , a benthic oligochaete, as a bioassay organism. In: A.L. Buikema and J. Cairns, Jr., eds

  7. Measurement of Antibody to Ureaplasma urealyticum by an Enzyme-Linked Immunosorbent Assay and Detection of Antibody Responses in Patients with Nongonococcal Urethritis

    PubMed Central

    Brown, Mary B.; Cassell, Gail H.; Taylor-Robinson, David; Shepard, Maurice C.

    1983-01-01

    The optimum conditions for the detection of human immunoglobulin G (IgG), IgM, and IgA antibodies to Ureaplasma urealyticum by an enzyme-linked immunosorbent assay (ELISA) were established by using a cell lysate antigen and commercially available alkaline phosphatase conjugates. No significant cross-reactions were observed among rabbit antisera to a variety of mycoplasmas of human origin and ureaplasma antigen, thus demonstrating the specificity of the ELISA. All human sera were assayed at a 1:200 dilution. Antigen was used at 20 μg of protein/ml and conjugates were diluted 1:500. Presence of IgG antibody to U. urealyticum was significantly associated with isolation of U. urealyticum (P < 0.001) in 110 women. Seventeen acute-phase and 19 convalescent-phase sera from male nongonococcal urethritis (NGU) patients were tested for the presence of antibody by both the metabolism inhibition assay and by ELISA, with overall agreements of 82 and 95% for acute- and convalescent-phase sera, respectively. Serum antibody responses were demonstrated to selected serotypes in the metabolism inhibition test, but the response as measured by the ELISA was independent of the serotype of the antigen used. Serum antibody levels in NGU patients were significantly higher (P < 0.002) than the normal serum standard in the IgG, IgM, and IgA classes. Additionally, the magnitude of change between acute- and convalescent-phase sera was greater for NGU patients than for normal asymptomatic ureaplasma-positive male controls. A significant change in antibody levels of one or more antibody classes was detected for 12 of 18 (67%) NGU patients by ELISA. Ten of the 12 (83%) individuals had a change in the IgM class, which is suggestive of an active infectious process. The ELISA is advantageous in that it requires only a single serotype antigen, uses one serum dilution, is class specific, and allows quantitative detection of differences between acute- and convalescent-phase sera. PMID:6833482

  8. [Research progress in pathogenicity of Ureaplasma urealyticum].

    PubMed

    Huang, Jun; Zhang, Jun; Song, Tiejun; Xie, Xinyou

    2013-07-01

    Ureaplasma urealyticum (UU) is closely related to human diseases including non-gonococcal urethritis (NGU), infertility, premature membranes and neonatal bronchopulmonary dysplasia. Researches on the pathogenicity of UU have become a hot topic in recent years, and suggest that many potential pathogenicity genes or putative pathogenicity islands are involved in its virulence. Moreover, the biovar and serum types of UU, the infection concentration and the state of the host immune system are also important to determine whether UU can cause human disease or not. In this article the recent progress of researches in the pathogenicity of UU is reviewed.

  9. Model of High-Energy-Density Battery Based on SiC Schottky Diodes

    DTIC Science & Technology

    2006-10-01

    ARL -D J M MILLER ATTN AMSRD- ARL -CI-OK-T TECHL PUB (2 COPIES ) ATTN AMSRD- ARL -CI-OK-TL TECHL LIB (2 COPIES) ATTN AMSRD- ARL -SE-DE M LITZ ...Model of High-Energy-Density Battery Based on SiC Schottky Diodes by Yves Ngu, Marc Litz , and Bruce Geil ARL -TR-3981 Ocotber 2006...Spectrum September 2004, 36-41. 6. Litz , M .; et al. On-Demand High Energy Density Materials. Amer. Inst. of Aero. and Astro. 7. Litz , M

  10. Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)

    PubMed Central

    Kang, Jaeyoun; Park, Changnam; Jang, Yeounghwan; Ahn, Meejung; Shin, Taekyun

    2016-01-01

    The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acetylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, Jacalin, PNA, ECL), complex type N-glycans (PHA-E and PHA-L), and fucose (UEA-I). Spores encased by a plasmodial membrane were labeled for the majority of these lectins, with the exception of LCA, PSA, PNA, and PHA-L. Four lectins (RCA 120, BSL-I, DBA, and SJA) belonging to the galactose/N-acetylgalactosamine group, only labeled spores, but not the plasmodial membrane. This is the first confirmation that various sugar residues are present in spores and plasmodial membranes of K. septempunctata ST3. PMID:27169676

  11. Subsets of epidermal Langerhans cells as defined by lectin binding profiles.

    PubMed

    Schuler, G; Romani, N; Linert, J; Shevach, E M; Stingl, G

    1983-11-01

    In this study we characterize the cell surface glycoconjugate moieties of strain 2 guinea pig epidermal Langerhans cells (LC) in single cell suspension by using a battery of 17 fluorescent lectins. All LC displayed binding sites for concanavalin A, succinylated concanavalin A, Lens culinaris agglutinin, Pisum sativum agglutinin, wheat germ agglutinin, succinylated wheat germ agglutinin, Griffonia simplicifolia agglutinin I, Ricinus communis agglutinin I, Phaseolus vulgaris E agglutinin, and Phaseolus vulgaris L agglutinin, but failed to bind Sophora japonica agglutinin (SJA), Dolichos biflorus agglutinin (DBA), and Ulex europaeus agglutinin I (UEA I). Neuraminidase pretreatment rendered LC reactive for SJA, but not for DBA and UEA I. The binding profiles of certain lectins point to the existence of LC subpopulations in that Griffonia simplicifolia I-B4 isolectin, peanut agglutinin (PNA), Helix pomatia agglutinin, and soybean agglutinin bound to only 80% (range 70-90%) of Ia-positive epidermal cells; binding sites for these lectins on primarily unreactive Ia-positive cells were unmasked when epidermal cells were treated with neuraminidase prior to lectin labeling. Ultrastructural PNA labeling studies revealed that the vast majority of Birbeck granule-containing LC displayed PNA binding sites, whereas indeterminate cells were consistently PNA-negative. Identification of carbohydrate configurations expressed on LC surfaces by lectin binding may provide a clue for the elucidation of the mechanisms of established LC functions and possibly the discovery of as yet unknown properties of this cell type.

  12. Western equatorial African forest-savanna mosaics: a legacy of late Holocene climatic change?

    NASA Astrophysics Data System (ADS)

    Ngomanda, A.; Chepstow-Lusty, A.; Makaya, M.; Favier, C.; Schevin, P.; Maley, J.; Fontugne, M.; Oslisly, R.; Jolly, D.

    2009-02-01

    Past vegetation and climate changes reconstructed using two pollen records from Lakes Maridor and Nguène, Gabon, provide new insights into the environmental history of western equatorial African rainforests during the last 4500 cal yr BP. The Lake Maridor pollen record indicates that the coastal savannas of western equatorial Africa did not exist during the mid-Holocene and instead the region was covered by evergreen rainforests. In the Lake Nguène pollen record, a rapid decline of hygrophilous evergreen rainforest occurred around 4000 cal yr BP, synchronously with grassland expansion around Lake Maridor. The establishment of coastal savannas in Gabon suggests decreasing humidity at the onset of the late Holocene. The marked reduction in evergreen rainforest and subsequent savanna expansion was associated with the colonization of secondary forests dominated by the palm, Elaeis guineensis, in the coastal region and the shrub, Alchornea cordifolia, further inland. A return to wetter climatic conditions from about 1400 cal yr BP led to the renewed spread of evergreen rainforest inland, whereas a forest-savanna mosaic still persists in the coastal region. There is no evidence to suggest that the major environmental changes observed are driven by human impact.

  13. Mycoplasma genitalium: An emerging sexually transmitted pathogen

    PubMed Central

    Sethi, Sunil; Singh, Gagandeep; Samanta, Palash; Sharma, Meera

    2012-01-01

    Mycoplasma genitalium is a member of genital mycoplasmas, which is emerging as an important causative agent of sexually transmitted infections both in males and females. The advent of polymerase chain reaction and other molecular methods have made studies on M. genitalium more feasible, which is otherwise a difficult organism to isolate. Besides Chlamydia trachomatis, M. genitalium is now an important and established cause of non gonococcal urethritis (NGU) in men, more so in persistent and recurrent NGU. Multiple studies have also shown a positive association of M. genitalium with mucopurulent cervicitis and vaginal discharge in females as well. The evidences for M. genitalium pelvic inflammatory diseases and infertility are quite convincing and indicate that this organism has potential to cause ascending infection. Lack of clear association with M. genitalium has been reported for bacterial vaginosis and adverse pregnancy outcomes. Diagnosis of M. genitalium infections is performed exclusively using nucleic acid amplification tests (NAATs), owing to poor or slow growth of bacterium in culture. Although there are no guidelines available regarding treatment, macrolide group of antimicrobials appear to be more effective than tetracyclines. The present review provides an overview of the epidemiology, pathogenesis, clinical presentation and management of sexually transmitted infections due to M. genitalium. PMID:23391789

  14. A Deep Analysis of the Small Non-Coding RNA Population in Schistosoma japonicum Eggs

    PubMed Central

    Cai, Pengfei; Piao, Xianyu; Hao, Lili; Liu, Shuai; Hou, Nan; Wang, Heng; Chen, Qijun

    2013-01-01

    Background Schistosoma japonicum is a parasitic flatworm that causes zoonotic schistosomiasis. The typical outcome of schistosomiasis is hepatic granuloma and fibrosis, which is primarily induced by soluble egg-derived antigens. Although schistosomal eggs represent an important pathogenic stage to the host, the biology of this critical stage is largely unknown. We previously investigated the expression profiles of sncRNAs during different developmental stages of this parasite. However, using small RNA extracted from egg-deposited liver tissues generated limited information about sncRNAs in eggs. Here, we characterized the complete small RNAome in this stage of the parasite after optimization of RNA purification. Methodology and Principal Findings A library, SjE, was constructed with the small RNA extracted from S. japonicum eggs and subjected to high-throughput sequencing. The data were depicted by comprehensive bioinformatic analysis to explore the expression features of sncRNAs in the egg stage. MicroRNAs accounted for about one quarter of the total small RNA population in this stage, with a strongly biased expression pattern of certain miRNA family members. Sja-miR-71, sja-miR-71-5p, and sja-miR-36-3p were suggested to play important roles in embryo development. A panel of transfer RNA fragments (tRFs) precisely processed from the 5′ end of mature tRNAs was identified for the first time, which represented a strong egg stage-biased expression. The tRNA-Ala derived small RNAs were the most highly expressed Sj-tRFs in eggs. Further, the expression of siRNAs from 29 types of well-defined transposable elements (TEs) was observed to be relatively stable among different developmental stages. Conclusions and Significance In this study, we characterized the sncRNA profile in the egg stage of S. japonicum. Featured expression of sncRNAs, especially the tRNA-derived small RNAs, was identified, which was further compared with that of other developmental stages. These

  15. Efficacy of standard therapies against Ureaplasma species and persistence among men with non-gonococcal urethritis enrolled in a randomised controlled trial.

    PubMed

    Khosropour, Christine M; Manhart, Lisa E; Gillespie, Catherine W; Lowens, M Sylvan; Golden, Matthew R; Jensen, Nicole L; Kenny, George E; Totten, Patricia A

    2015-08-01

    Ureaplasma urealyticum biovar 2 (UU-2), but not Ureaplasma parvum (UP), has been associated with non-gonococcal urethritis (NGU), but little is known about species-specific responses to standard therapies. We examined species-specific treatment outcomes and followed men with treatment failure for 9 weeks. From May 2007 to July 2011, men aged ≥16 attending a sexually transmitted disease (STD) clinic in Seattle, Washington, with NGU (urethral discharge or urethral symptoms plus ≥5 polymorphonuclear leucocytes /high-powered field) enrolled in a double-blind, randomised trial. Participants received active azithromycin (1 g) + placebo doxycycline or active doxycycline (100 mg twice a day ×7 days) + placebo azithromycin. Ureaplasma were detected in culture followed by species-specific PCR. Outcomes were assessed at 3, 6 and 9 weeks. At 3 weeks, men with persistent Ureaplasma detection received 'reverse therapy' (e.g., active doxycycline if they first received active azithromycin). At 6 weeks, persistently positive men received moxifloxacin (400 mg×7 days). Of 490 men, 107 (22%) and 60 (12%) were infected with UU-2 and UP, respectively, and returned at 3 weeks. Persistent detection was similar for UU-2-infected men initially treated with azithromycin or doxycycline (25% vs. 31%; p=0.53), but differed somewhat for men with UP (45% vs. 24%; p=0.11). At 6 weeks, 57% of UU-2-infected and 63% of UP-infected men who received both drugs had persistent detection. Failure after moxifloxacin occurred in 30% and 36%, respectively. Persistent detection of UU-2 or UP was not associated with signs/symptoms of NGU. Persistent detection after treatment with doxycycline, azithromycin and moxifloxacin was common for UU and UP, but not associated with persistent urethritis. NCT00358462. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  16. Effect of low dose ionising radiation and nitric oxide on the state of animal blood system.

    PubMed

    Rodionova, N; Ganzha, O; Makovetska, L; Druzhyna, M; Muzalov, I; Mikhailenko, V

    2013-01-01

    Meta: doslidyty tryvalyj vplyv oksydu azotu ta malyh doz ionizujuchoi' radiacii' na stan systemy krovi. Materialy i metody. V eksperymenti vykorystani neinbredni samci-shhury vagoju 120–140 g. Tvaryny zaznavaly dii' riznyh chynnykiv: ingaljacii' oksydom azotu protjagom 30 dib (po 14 godyn na dobu), frakcionovanogo oprominennja malymy dozamy ionizujuchoi' radiacii' v sumarnij dozi 1,0 Gr ta i'h sumisnogo vplyvu. Doslidzhuvaly gematologichni, biohimichni ta biofizychni pokaznyky. Rezul'taty doslidzhennja. Pokazana radiozahysna dija oksydu azotu na morfofunkcional'ni pokaznyky, shho projavljalos' u reakcii' erytroi'dnogo pulu krovotvorennja i limfocytarnoi' lanky, u rivni porushennja okysnogo metabolizmu, znyzhenni vmistu atypovyh limfocytiv u peryferychnij krovi. Vysnovok. Oksyd azotu za sumisnoi' prolongovanoi' dii' z malymy dozamy ionizujuchogo vyprominjuvannja vyjavljaje radiozahysnyj efekt u systemi krovi. Kombinovana dija zaznachenyh chynnykiv, shho harakteryzujut'sja vplyvom na rizni metabolichni shljahy, pryzvodyt' do bil'sh efektyvnoi' adaptacii', nizh i'h okreme zastosuvannja.

  17. Testing and validation of methods for dose measuring in anisotropic fields under occupational exposure in interventional cardiology.

    PubMed

    Voloskyi, V N; Chumak, V V; Bakhanova, O V; Morgun, A A

    2013-01-01

    Metoju roboty bula eksperymental'na perevirka praktychnogo zastosuvannja rozroblenoi' dlja umov AES metodyky ocinky indyvidual'noi' efektyvnoi' dozy za pokazannjamy chotyr'oh dozymetriv v umovah neodnoridnogo zovnishn'ogo gamma-oprominennja z vidomymy kutovymy harakterystykamy polja vyprominjuvannja. Materialy ta metody doslidzhen': provedeno dvi serii' eksperymental'nyh fantomnyh doslidzhen' v umovah rentgen-operacijnoi' na robochyh miscjah likariv intervencijnoi' kardiologii' pid chas provedennja operacij. Rezul'taty ta vysnovky: pokazano, shho dozovi polja v tili medychnogo pracivnyka harakteryzujut'sja znachnymy gradijentamy, pokazannja indyvidual'nyh dozymetriv, zalezhno vid miscja roztashuvannja, mozhut' vidriznjatysja vid 1,5 do 10 raziv, otzhe, vykorystannja odnogo dozymetra ne daje dostatn'oi' informacii' dlja dostovirnoi' ocinky efektyvnoi' dozy. Doslidzhennja pidtverdyly mozhlyvist' zastosuvannja metodyky AES na robochomu misci intervencijnogo kardiologa. Prodemonstrovano neobhidnist' kontrolju doz na kryshtalyk oka.

  18. Characterization of a Charge Injection Device Camera System for Spectrochemical Measurements.

    DTIC Science & Technology

    1985-11-25

    sj.L 40 aflJLA ke *U0LILDDLdde LeSJaA~ufl j04 a [qPe.ns IOU aJP Sa-DA,3p iusi inq ’sanbLUq:.gZ :)do:)soj;:.ads LP:)4i Leut ) 40 sad,(i awos joj aSLwojd...AD-AI62 472 CHARACTERIZATION OF A CHARGE INJECTION DEVICE CAMERA i/7 .524SYSTEM FOR SPECTROCH (U) ARIZONA UNIV TUCSON DEPT OF 1)~CHEMISTRY G2 R SINS...TYOE OF REPORT &PERIOD CO VCRC0 , aracterization of a Charge Injection Device eri Cu17era System for Spect-roahemical M.easurements . EORMING ORG

  19. Research and design of intelligent distributed traffic signal light control system based on CAN bus

    NASA Astrophysics Data System (ADS)

    Chen, Yu

    2007-12-01

    Intelligent distributed traffic signal light control system was designed based on technologies of infrared, CAN bus, single chip microprocessor (SCM), etc. The traffic flow signal is processed with the core of SCM AT89C51. At the same time, the SCM controls the CAN bus controller SJA1000/transceiver PCA82C250 to build a CAN bus communication system to transmit data. Moreover, up PC realizes to connect and communicate with SCM through USBCAN chip PDIUSBD12. The distributed traffic signal light control system with three control styles of Vehicle flux, remote and PC is designed. This paper introduces the system composition method and parts of hardware/software design in detail.

  20. Advanced high performance horizontal piezoelectric hybrid synthetic jet actuator

    NASA Technical Reports Server (NTRS)

    Xu, Tian-Bing (Inventor); Jiang, Xiaoning (Inventor); Su, Ji (Inventor)

    2012-01-01

    The present invention comprises a high performance, horizontal, zero-net mass-flux, synthetic jet actuator for active control of viscous, separated flow on subsonic and supersonic vehicles. The present invention is a horizontal piezoelectric hybrid zero-net mass-flux actuator, in which all the walls of the chamber are electrically controlled synergistically to reduce or enlarge the volume of the synthetic jet actuator chamber in three dimensions simultaneously and to reduce or enlarge the diameter of orifice of the synthetic jet actuator simultaneously with the reduction or enlargement of the volume of the chamber. The present invention is capable of installation in the wing surface as well as embedding in the wetted surfaces of a supersonic inlet. The jet velocity and mass flow rate for the SJA-H will be several times higher than conventional piezoelectric actuators.

  1. Design of a CAN bus interface for photoelectric encoder in the spaceflight camera

    NASA Astrophysics Data System (ADS)

    Sun, Ying; Wan, Qiu-hua; She, Rong-hong; Zhao, Chang-hai; Jiang, Yong

    2009-05-01

    In order to make photoelectric encoder usable in a spaceflight camera which adopts CAN bus as the communication method, CAN bus interface of the photoelectric encoder is designed in this paper. CAN bus interface hardware circuit of photoelectric encoder consists of CAN bus controller SJA 1000, CAN bus transceiver TJA1050 and singlechip. CAN bus interface controlling software program is completed in C language. A ten-meter shield twisted pair line is used as the transmission medium in the spaceflight camera, and speed rate is 600kbps.The experiments show that: the photoelectric encoder with CAN bus interface which has the advantages of more reliability, real-time, transfer rate and transfer distance overcomes communication line's shortcomings of classical photoelectric encoder system. The system works well in automatic measuring and controlling system.

  2. Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra)

    PubMed Central

    CARMANCHAHI, P. D.; FERRARI, C. C.; ALDANA MARCOS, H. J.; AFFANNI, J. M.; SONEZ, C. A.; PAZ, D. A.

    2000-01-01

    Conventional carbohydrate histochemistry and the binding patterns of 21 lectins were analysed to characterise the glycoconjugate content in the components of the vomeronasal organ of the armadillo Chaetophractus villosus. The mucomicrovillous complex of the sensory epithelium bound most of the lectins studied. No reaction was observed with Con A, PSA, S-Con A and SBA, and the sustentacular cells were stained with UEA-I, DSL, LEL, STL and Con A. The vomeronasal receptor neurons were labelled with S-WGA, WGA, PNA, UEA-I, STL, Con A, S-Con A, ECL and RCA120. The basal cell layer reacted with S-WGA, WGA, LCA, UEA-I, DSL, LEL, STL, Con A, JAC and VVA. The nonsensory epithelium exhibited a differential staining in relation to the different components. The mucociliary complex stained with ECL, DBA, JAC, RCA120, STL, LCA, PHA-E, PHA-L, LEL, BSL-I and VVA. However, SJA and UEA-I stained the mucus complex lining a subpopulation of columnar cells. The cytoplasm and cell membranes of columnar cells was labelled with DBA, DSL and LCA. The apical region of these cells exhibited moderate reactivity with LEL and SJA. None of the lectins bound specifically to secretory granules of the nonsecretory cells. Basal cells of the nonsensory epithelium were labelled with DSL, LEL, LCA, BSL-I and STL. The vomeronasal glands showed a positive reaction with WGA, DSL, LEL, LCA, DBA, PNA, RCA120 and SBA. Subpopulations of acinar cells were observed with ECL, S-WGA, Con A, S-Con A and DBA. PNA and RCA120 stained the cells lining the glandular ducts. In comparison with previous results obtained in the olfactory mucosa of the same group of armadillos, the carbohydrate composition of the vomeronasal organ sensory epithelium differed from the olfactory sensory epithelium. This is probably related to the different nature of molecules involved in the perireceptor processes. PMID:10853958

  3. Understanding Grammars through Diachronic Change

    PubMed Central

    Madariaga, Nerea

    2017-01-01

    In this paper, I will vindicate the importance of syntactic change for the study of synchronic stages of natural languages, according to the following outline. First, I will analyze the relationship between the diachrony and synchrony of grammars, introducing some basic concepts: the notions of I-language/E-language, the role of Chomsky's (2005) three factors in language change, and some assumptions about language acquisition. I will briefly describe the different approaches to syntactic change adopted in generative accounts, as well as their assumptions and implications (Lightfoot, 1999, 2006; van Gelderen, 2004; Biberauer et al., 2010; Roberts, 2012). Finally, I will illustrate the convenience of introducing the diachronic dimension into the study of at least certain synchronic phenomena with the help of a practical example: variation in object case marking of several verbs in Modern Russian, namely, the verbs denoting avoidance and the verbs slušat'sja “obey” and dožidat'sja “expect,” which show two object case-marking patterns, genitive case in standard varieties and accusative case in colloquial varieties. To do so, I will review previous descriptive and/or functionalist accounts on this or equivalent phenomena (Jakobson, 1984 [1936]; Clancy, 2006; Nesset and Kuznetsova, 2015a,b). Then, I will present a formal—but just synchronic—account, applying Sigurðsson (2011) hypothesis on the expression of morphological case to this phenomenon. Finally, I will show that a formal account including the diachronic dimension is superior (i.e., more explanative) than purely synchronic accounts. PMID:28824474

  4. Blow-up in p-Laplacian heat equations with nonlinear boundary conditions

    NASA Astrophysics Data System (ADS)

    Ding, Juntang; Shen, Xuhui

    2016-10-01

    In this paper, we investigate the blow-up of solutions to the following p-Laplacian heat equations with nonlinear boundary conditions: {l@{quad}l}(h(u))_t =nabla\\cdot(|nabla u|pnabla u)+k(t)f(u) &{in } Ω×(0,t^{*}), |nabla u|ppartial u/partial n=g(u) &on partialΩ×(0,t^{*}), u(x,0)=u0(x) ≥ 0 & {in } overline{Ω},. where {p ≥ 0} and {Ω} is a bounded convex domain in {RN}, {N ≥ 2} with smooth boundary {partialΩ}. By constructing suitable auxiliary functions and using a first-order differential inequality technique, we establish the conditions on the nonlinearities and data to ensure that the solution u( x, t) blows up at some finite time. Moreover, the upper and lower bounds for the blow-up time, when blow-up does occur, are obtained.

  5. Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology

    PubMed Central

    Tabrizi, Sepehr N.; Tan, Lit Y.; Walker, Samantha; Twin, Jimmy; Poljak, Marin; Bradshaw, Catriona S.; Fairley, Christopher K.; Bissessor, Melanie; Mokany, Elisa; Todd, Alison V.; Garland, Suzanne M.

    2016-01-01

    Mycoplasma genitalium is a cause of non-gonoccocal urethritis (NGU) in men and cervicitis and pelvic inflammatory disease in women. Recent international data also indicated that the first line treatment, 1 gram stat azithromycin therapy, for M. genitalium is becoming less effective, with the corresponding emergence of macrolide resistant strains. Increasing failure rates of azithromycin for M. genitalium has significant implications for the presumptive treatment of NGU and international clinical treatment guidelines. Assays able to predict macrolide resistance along with detection of M. genitalium will be useful to enable appropriate selection of antimicrobials to which the organism is susceptible and facilitate high levels of rapid cure. One such assay recently developed is the MG 23S assay, which employs novel PlexZyme™ and PlexPrime™ technology. It is a multiplex assay for detection of M. genitalium and 5 mutations associated with macrolide resistance. The assay was evaluated in 400 samples from 254 (186 males and 68 females) consecutively infected participants, undergoing tests of cure. Using the MG 23S assay, 83% (331/440) of samples were positive, with 56% of positives carrying a macrolide resistance mutation. Comparison of the MG 23S assay to a reference qPCR method for M. genitalium detection and high resolution melt analysis (HRMA) and sequencing for detection of macrolide resistance mutations, resulted in a sensitivity and specificity for M. genitalium detection and for macrolide resistance of 99.1/98.5% and 97.4/100%, respectively. The MG 23S assay provides a considerable advantage in clinical settings through combined diagnosis and detection of macrolide resistance. PMID:27271704

  6. Biology and Trapping of Stable Flies (Diptera: Muscidae) Developing in Pineapple Residues (Ananas comosus) in Costa Rica

    PubMed Central

    Solórzano, José-Arturo; Gilles, Jeremie; Bravo, Oscar; Vargas, Cristina; Gomez-Bonilla, Yannery; Bingham, Georgina V.; Taylor, David B.

    2015-01-01

    Pineapple production in Costa Rica increased nearly 300-fold during the last 30 yr, and >40,000 hectares of land are currently dedicated to this crop. At the end of the pineapple cropping cycle, plants are chopped and residues incorporated into the soil in preparation for replanting. Associated with increased pineapple production has been a large increase in stable fly, Stomoxys calcitrans (L.), populations. Stable flies are attracted to, and oviposit in, the decomposing, chopped pineapple residues. In conjunction with chemical control of developing larvae, adult trapping is an important control strategy. In this study, four blue-black fabric traps, Nzi, Vavoua, Model H, and Ngu, were compared with a white sticky trap currently used for stable fly control in Costa Rica. Overall, the white sticky trap caught the highest number of stable flies, followed by the Nzi, Vavoua, Model H, and Ngu. Collections on the white sticky trap increased 16 d after residues were chopped; coinciding with the expected emergence of flies developing in the pineapple residues. During this same time period, collections in the blue-black fabric traps decreased. Sex ratio decreased from >7:1 (females:males) 3–7 d after chopping to 1:1 at 24–28 d. White sticky, Nzi and Vavoua traps collected similar numbers of colonizing flies 3–7 d after residues were chopped. However, white sticky traps collected more flies once emergence from the pineapple residues began. Although white sticky traps collected more flies than fabric traps, they remain labor intensive and environmentally unsound because of their disposable and nonbiodegradable nature. PMID:26454479

  7. Biology and trapping of stable flies (Diptera: Muscidae) developing in pineapple residues (Ananas comosus) in Costa Rica.

    PubMed

    Solórzano, José-Arturo; Gilles, Jeremie; Bravo, Oscar; Vargas, Cristina; Gomez-Bonilla, Yannery; Bingham, Georgina V; Taylor, David B

    2015-01-01

    Pineapple production in Costa Rica increased nearly 300-fold during the last 30 yr, and >40,000 hectares of land are currently dedicated to this crop. At the end of the pineapple cropping cycle, plants are chopped and residues incorporated into the soil in preparation for replanting. Associated with increased pineapple production has been a large increase in stable fly, Stomoxys calcitrans (L.), populations. Stable flies are attracted to, and oviposit in, the decomposing, chopped pineapple residues. In conjunction with chemical control of developing larvae, adult trapping is an important control strategy. In this study, four blue-black fabric traps, Nzi, Vavoua, Model H, and Ngu, were compared with a white sticky trap currently used for stable fly control in Costa Rica. Overall, the white sticky trap caught the highest number of stable flies, followed by the Nzi, Vavoua, Model H, and Ngu. Collections on the white sticky trap increased 16 d after residues were chopped; coinciding with the expected emergence of flies developing in the pineapple residues. During this same time period, collections in the blue-black fabric traps decreased. Sex ratio decreased from >7:1 (females:males) 3-7 d after chopping to 1:1 at 24-28 d. White sticky, Nzi and Vavoua traps collected similar numbers of colonizing flies 3-7 d after residues were chopped. However, white sticky traps collected more flies once emergence from the pineapple residues began. Although white sticky traps collected more flies than fabric traps, they remain labor intensive and environmentally unsound because of their disposable and nonbiodegradable nature. Published by Oxford University Press on behalf of the Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  8. Remarkable increase in fluoroquinolone-resistant Mycoplasma genitalium in Japan.

    PubMed

    Kikuchi, Mina; Ito, Shin; Yasuda, Mitsuru; Tsuchiya, Tomohiro; Hatazaki, Kyoko; Takanashi, Masaki; Ezaki, Takayuki; Deguchi, Takashi

    2014-09-01

    We determined the prevalence of macrolide and fluoroquinolone resistance-associated mutations in Mycoplasma genitalium DNA specimens from men with non-gonococcal urethritis (NGU) and analysed their effects on antibiotic treatments of M. genitalium infections. In this retrospective study, we examined antibiotic resistance-associated mutations in the 23S rRNA, gyrA and parC genes of M. genitalium and the association of the mutations with microbiological outcomes of antibiotic treatments in men with M. genitalium-positive NGU. No macrolide resistance-associated mutations in the 23S rRNA gene were observed in 27 M. genitalium DNA specimens in 2011 and in 24 in 2012. However, 5 of 17 in 2013 had 23S rRNA mutations. Three of 15 in 2011, 6 of 19 in 2012 and 8 of 17 in 2013 had fluoroquinolone resistance-associated alterations in ParC. Three in 2013 had both the antibiotic resistance-associated alterations coincidentally. In two men with M. genitalium harbouring 23S rRNA mutations, the mycoplasma persisted after treatment with a regimen of 2 g of extended-release azithromycin (AZM-SR) once daily for 1 day. All nine men with mycoplasma harbouring ParC alterations were microbiologically cured with a regimen of 100 mg of sitafloxacin twice daily for 7 days. Macrolide- or fluoroquinolone-resistant M. genitalium appears to be increasing, and the increase in fluoroquinolone-resistant mycoplasmas is especially remarkable in Japan. Mycoplasmas harbouring 23S rRNA mutations would be resistant to the AZM-SR regimen, but those harbouring ParC alterations would still be susceptible to the sitafloxacin regimen. © The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  9. Meatal Swabs Contain Less Cellular Material and Are Associated with a Decrease in Gram Stain Smear Quality Compared to Urethral Swabs in Men.

    PubMed

    Jordan, Stephen J; Schwebke, Jane R; Aaron, Kristal J; Van Der Pol, Barbara; Hook, Edward W

    2017-07-01

    Urethral swabs are the samples of choice for point-of-care Gram stain testing to diagnose Neisseria gonorrhoeae infection and nongonococcal urethritis (NGU) in men. As an alternative to urethral swabs, meatal swabs have been recommended for the collection of urethral discharge to diagnose N. gonorrhoeae and Chlamydia trachomatis infection in certain populations by nucleic acid amplification testing (NAAT), as they involve a less invasive collection method. However, as meatal swabs could be sampling a reduced surface area and result in fewer collected epithelial cells compared to urethral swabs, the adequacy of meatal swab specimens to collect sufficient cellular material for Gram stain testing remains unknown. We enrolled 66 men who underwent either urethral or meatal swabbing and compared the cellular content and Gram stain failure rate. We measured the difference in swab cellular content using the Cepheid Xpert CT/NG sample adequacy control crossing threshold (SACCT) and determined the failure rate of Gram stain smears (GSS) due to insufficient cellular material. In the absence of discharge, meatal smears were associated with a significant reduction in cellular content (P = 0.0118), which corresponded with a GSS failure rate significantly higher than that for urethral swabs (45% versus 3%, respectively; P < 0.0001). When discharge was present, there was no difference among results from urethral and meatal swabs. Therefore, if GSS testing is being considered for point-of-care diagnosis of N. gonorrhoeae infection or NGU in men, meatal swabs should be avoided in the absence of a visible discharge. Copyright © 2017 American Society for Microbiology.

  10. Performance evaluation of automated urine microscopy as a rapid, non-invasive approach for the diagnosis of non-gonococcal urethritis

    PubMed Central

    Pond, Marcus J; Nori, Achyuta V; Patel, Sheel; Laing, Ken; Ajayi, Margarita; Copas, Andrew J; Butcher, Philip D; Hay, Phillip; Sadiq, Syed Tariq

    2015-01-01

    Objectives Gram-stained urethral smear (GSUS), the standard point-of-care test for non-gonococcal urethritis (NGU) is operator dependent and poorly specific. The performance of rapid automated urine flow cytometry (AUFC) of first void urine (FVU) white cell counts (UWCC) for predicting Mycoplasma genitalium and Chlamydia trachomatis urethral infections was assessed and its application to asymptomatic infection was evaluated. Methods Receiver operating characteristic curve analysis, determining FVU-UWCC threshold for predicting M. genitalium or C. trachomatis infection was performed on 208 ‘training’ samples from symptomatic patients and subsequently validated using 228 additional FVUs obtained from prospective unselected patients. Results An optimal diagnostic threshold of >29 UWC/µL gave sensitivities and specificities for either infection of 81.5% (95% CI 65.1% to 91.6%) and 85.8% (79.5% to 90.4%), respectively, compared with 86.8% (71.1% to 95%) and 64.7% (56.9% to 71.7%), respectively, for GSUS, using the training set samples. FVU-UWCC demonstrated sensitivities and specificities of 69.2% (95% CI 48.1% to 84.9%) and 92% (87.2% to 95.2%), respectively, when using validation samples. In asymptomatic patients where GSUS was not used, AUFC would have enabled more infections to be detected compared with clinical considerations only (71.4% vs 28.6%; p=0.03). The correlation between UWCC and bacterial load was stronger for M. genitalium compared with C. trachomatis (τ=0.426, p≤0.001 vs τ=0.295, p=0.022, respectively). Conclusions AUFC offers improved specificity over microscopy for predicting C. trachomatis or M. genitalium infection. Universal AUFC may enable non-invasive diagnosis of asymptomatic NGU at the PoC. The degree of urethral inflammation exhibits a stronger association with pathogen load for M. genitalium compared with C. trachomatis. PMID:25614466

  11. Sexually transmitted diseases in Poland in 2011.

    PubMed

    Majewski, Sławomir; Rudnicka, Iwona

    2013-01-01

    Was to assess epidemiological situation of sexually transmitted diseases in Poland in 2011. Analysis of the data on prevalence of syphilis, gonorrhoea, non-gonococcal urethritis, genital herpes and genital warts was gathered from yearly reports MZ-14 from several didtricts. In 2011 there were 841 reported cases of all types of syphilis, which was higher by 24 than in 2010. Within the reported cases, 554 cases were of early syphilis, 274 cases of late syphilis, and 11 cases of congenital syphilis. Syphilis during pregnancy and childbirth was reported in 13 women. The highest incidence of syphilis in 2011, similarly to previous years was in Mazowieckie district (4.7/100,000) and the lowest in Swietokrzyskie district (0.2/100,000), the average in a whole country accounted to 2.2/100,000. In 2011 there were 351 cases of gonorrhoea reported, which was higher by 77 cases than reported in the previous year. The highest incidence was reported in Mazowieckie voivodeship. Non-gonococcal urethritis -NGU was identified in 484 persons; this was less by 294 cases than reported in the previous year. The highest incidence rate was reported in Dolnoślaskie voivodeship 9.6/100,000. There were 428 cases of genital warts reported which was less by 174 cases than reported in 2010. The highest incidence rate was reported in Mazowieckie voivodeship, Warminsko- Mazurskie voivodeship and Kujawsko-Pomorskie voivodeship. As in previous years the most unfavourable epidemiological situation in terms of all registered sexually transmitted diseases was in Mazowieckie voivodeship. In 2011 among sexually transmitted diseases NGU and genital warts were reported in lower numbers then in previous year. In the same time numbers of reported cases of gonorrhoea and syphilis increased. There is a continued decrease in the number of serological tests done for syphilis. Epidemiological indicators of treatment for gonorrhoea and syphilis are very low for number of years. The epidemiological data is

  12. Integrated actuation and energy harvesting in prestressed piezoelectric synthetic jets

    NASA Astrophysics Data System (ADS)

    Mane, Poorna

    With the looming energy crisis compounded by the global economic downturn there is an urgent need to increase energy efficiency and to discover new energy sources. An approach to solve this problem is to improve the efficiency of aerodynamic vehicles by using active flow control tools such as synthetic jet actuators. These devices are able to reduce fuel consumption and streamlined vehicle design by reducing drag and weight, and increasing maneuverability. Hence, the main goal of this dissertation is to study factors that affect the efficiency of synthetic jets by incorporating energy harvesting into actuator design using prestressed piezoelectric composites. Four state-of-the-art piezoelectric composites were chosen as active diaphragms in synthetic jet actuators. These composites not only overcome the inherent brittle and fragile nature of piezoelectric materials but also enhance domain movement which in turn enhances intrinsic contributions. With these varying characteristics among different types of composites, the intricacies of the synthetic jet design and its implementation increases. In addition the electrical power requirements of piezoelectric materials make the new SJA system a coupled multiphysics problem involving electro-mechanical and structural-fluid interactions. Due to the nature of this system, a design of experiments approach, a method of combining experiments and statistics, is utilized. Geometric and electro-mechanical factors are investigated using a fractional factorial design with peak synthetic jet velocity as a response variable. Furthermore, energy generated by the system oscillations is harvested with a prestressed composite and a piezo-polymer. Using response surface methodology the process is optimized under different temperatures and pressures to simulate harsh environmental conditions. Results of the fractional factorial experimental design showed that cavity dimensions and type of signal used to drive the synthetic jet actuator

  13. Implementation of numerical simulations for rockfall hazard mapping in the Norddal municipality, Norway.

    NASA Astrophysics Data System (ADS)

    Yugsi Molina, Freddy Xavier; Oppikofer, Thierry; Otterå, Solveig; Hermanns, Reginald; Taurisano, Andrea; Wasrud, Jaran; Are Jensen, Odd; Rødseth Kvakland, Marte

    2013-04-01

    The Norwegian Water Resources and Energy Directorate (NVE) in cooperation with the Geological Survey of Norway (NGU) are implementing a nationwide program to systematically produce hazard maps for rockfalls, debris flows and snow avalanches in steep terrains. Activities during this program mapping are being carried out by both institutions, and for some areas, outsourced to the private sector. The results presented in this contribution focus on the rockfall component only, and are part of the hazard mapping activities carried out by NGU. Results from all parties involved will further lead in future, in combination with the components on debris flows and snow avalanches, to the preparation of guidelines for landslide hazard mapping. Those will be presented and recommended for the use of private consultants that work on municipality level. The first goal of the project is the preparation of hazard maps for critical areas where a large number of people are exposed to the threat of such type of mass movements. Results from a pilot area in Sylte (Norddal municipality) were presented in the EGU general assembly in 2012. The main objective of this contribution is to present the first finished rockfall hazard maps generated by NGU during the execution of the program. The results presented in this contribution were obtained for the Norddal municipality (Møre og Romsdal county). The area was selected based on the hazard mapping plan of Norway published in 2011, where Norddal is considered a priority area. The area is located in a valley over-steepened by glacial erosion that is characterized by high cliffs of medium to coarse-grained quartz-dioritic to granitic gneisses of Proterozoic age. Multiple scree deposits product of older and recent rockfall activity can be seen along the bottom at both valley flanks. Sylte, the main locality in the Norddal municipality, is located at the valley outlet to the fjord. Several other smaller localities are found along the valley. A

  14. The SPARDIG project - Transforming analogue sparker records from the Norwegian continental shelf into SEG-Y format, first results

    NASA Astrophysics Data System (ADS)

    Schaming, Marc; Rise, Leif; Chand, Shyam; Reidulv, Bøe; Terje Osmundsen, Per; Redfield, Tim

    2017-04-01

    A large number of sparker lines were acquired on the Norwegian continental shelf during the years 1970-1982, by IKU (Sintef Petroleum Research). The responsibility of the analogue seismic database was transferred to NGU in 1998; this included storage of the physical data (original paper rolls and half-scale film copies) and the digital navigation database. The data (from 60°N to 71°30N) were in the early eighties subdivided in 6 data packages, and offered for sale to oil companies as half scale folded paper copies (25 cm width). Navigation applied was mainly Decca Main Chain. The 2014-2016 SPARDIG project (Chand et al., 2016) was supported by NGU, AkerBP (Det Norske), Lundin Norway and the Seabed Project. In the project, IPGS has transformed 374 rolls of analogue sparker lines in 17 different surveys into SEG-Y format. The total length of converted survey lines is 31 261 kilometers. Rolls were scanned at 600 dpi and converted into SEG-Y using the SeisTrans (Caldera software) application (Miles et al., 2007). SeisTrans uses interactive, iterative and repeatable steps in a dedicated graphics window. A first step allows definition of axes and scales, then record time lines (horizontal TWT times and navigation time lines down the record) are picked and removed, and traces are defined. At this step, control tools are available to ensure the quality of the traces. After that, navigation information extracted and interpolated from excel files are added to trace headers. A continuous QC process allows production of SEG-Y files directly readable by interpretation software. The SEG-Y data will be delivered to the Norwegian Discos National Repository (https://portal.diskos.cgg.com/whereoil-data/) but access will be restricted to participants until 1st April 2019. IKU sparker lines have higher resolution than conventional 2D lines, but the penetration is limited. The data sets are complementary to each other. In 2D seismic lines, it is often difficult to delineate units in

  15. NASA's New Millennium ST-8 Mission

    NASA Astrophysics Data System (ADS)

    Bothwell, M.; Stevens, C. M.; Chmielewski, A. B.; Fujita, T.; Minning, C.; Nelson, R. M.; Ku, J.; McEachen, M. E.; Sampson, J. R.

    2005-12-01

    NASA's Space Technology 8 project (ST-8) is subsystem demonstration which will validate four technologies that have been identified as necessary to enable future NASA space science missions. NASA's New Millennium Program, with input from the science community, identified the following four technologies: Miniature Loop Heat Pipe with Multiple Evaporators Thermal Management System (MLHP), Scalable Architecture for the Investigation of the Load Managing Attributes of a Slender Truss (SAILMAST), Next Generation UltraFlex (NGU), and Environmentally Adaptive Fault Tolerant Computing (EAFTC). Once validated on a deep space mission, these technologies will be available for use on future science missions. Examples of previous enabling technologies demonstrated by NMP are the ion engines on Deep Space 1 and the Autonomous Sciencecraft Experiment demonstrated by ST6. The MLHP, provided by NASA's Goddard Space Flight Center will demonstrate that a loop heat pipe with multiple evaporators and condensers can transport large heat loads over long distances without external pumping. It is expected that this technology will enable more precise temperature control decreasing the mass, power, and volume of small remote sensing and surface-based spacecraft. The SAILMAST experiment is a deployable gossamer mast technology, which will validate its load-carrying characteristics by correlating in-flight measurements with analytical predictions. It will enable a new class of missions, which employ solar sail propulsion technology. The NGU will demonstrate the next generation in ultra-lightweight fan-folded flexible solar arrays, providing ultra-high specific power (170-220 W/kg BOL), ultra-compact stowage volume (>33 W/m3), and high deployed stiffness. The result is less mass and volume needed for power generation on future spacecraft. The EAFTC will integrate commercial-off-the-shelf (COTS) processing components and fault-tolerant control algorithms to provide an adaptable, high

  16. Management of non-gonococcal urethritis.

    PubMed

    Moi, Harald; Blee, Karla; Horner, Patrick J

    2015-07-29

    Non-gonococcal urethritis (NGU), or inflammation of the urethra, is the most common treatable sexually transmitted syndrome in men, with approximately 20-50 % of cases being due to infection with Chlamydia trachomatis and 10-30 % Mycoplasma genitalium. Other causes are Ureaplasma urealyticum, Trichomonas vaginalis, anaerobes, Herpes simplex virus (HSV) and adenovirus. Up to half of the cases are non-specific. Urethritis is characterized by discharge, dysuria and/or urethral discomfort but may be asymptomatic. The diagnosis of urethritis is confirmed by demonstrating an excess of polymorpho-nuclear leucocytes (PMNLs) in a stained smear. An excess of mononuclear leucocytes in the smear indicates a viral etiology. In patients presenting with symptoms of urethritis, the diagnosis should be confirmed by microscopy of a stained smear, ruling out gonorrhea. Nucleid acid amplifications tests (NAAT) for Neisseria gonorrhoeae, C. trachomatis and for M. genitalium. If viral or protozoan aetiology is suspected, NAAT for HSV, adenovirus and T. vaginalis, if available. If marked symptoms and urethritis is confirmed, syndromic treatment should be given at the first appointment without waiting for the laboratory results. Treatment options are doxycycline 100 mg x 2 for one week or azithromycin 1 gram single dose or 1,5 gram distributed in five days. However, azithromycin as first line treatment without test of cure for M. genitalium and subsequent Moxifloxacin treatment of macrolide resistant strains will select and increase the macrolide resistant strains in the population. If positive for M. genitalium, test of cure samples should be collected no earlier than three weeks after start of treatment. If positive in test of cure, moxifloxacin 400 mg 7-14 days is indicated. Current partner(s) should be tested and treated with the same regimen. They should abstain from intercourse until both have completed treatment. Persistent or recurrent NGU must be confirmed with microscopy

  17. Comparative Gut Microbiomes of Four Species Representing the Higher and the Lower Termites.

    PubMed

    Su, LiJuan; Yang, LeLe; Huang, Shi; Su, XiaoQuan; Li, Yan; Wang, FengQin; Wang, EnTao; Kang, Ning; Xu, Jian; Song, AnDong

    2016-01-01

    Aiming at learning the association between the gut microbiota and termites with different diet habits and phylogenetic positions, the gut bacteria of three populations for each of the two higher termites (wood-feeding Mironasutitermes shangchengensis and fungus-feeding Odontotermes formosanus) and two wood-feeding lower termites (Tsaitermes ampliceps and Reticulitermes flaviceps) were analyzed by high-throughput 454 pyrosequencing of 16S V1-V3 amplicons. As results, 132 bacterial genera and some unidentified operational taxonomic units within 29 phyla in the gut bacteria were detected, with Spirochaetes (11-55%), Firmicutes (7-18%), Bacteroidetes (7-31%), and Proteobacteria (8-14%) as the main phyla, and Treponema, TG5, Dysgonomonas, Tannerella, za29, Lactococcus, Pseudomonas, and SJA-88 as the common genera in all the four termites. The diversity of gut bacterial communities in the higher termite guts was significantly greater than that in the lower termites; while the gut microbiota in M. shangchengensis (wood-feeding higher termite) was more similar to those of the wood-feeding lower termites rather than that of O. formosanus (fungus-feeding higher termite), and phylum Spirochaetes and nitrogen-fixing bacteria were super-dominant in the wood-feeding termites, despite of their phylogenetic relations. This study reported for the first time the gut bacterial communities for the termites of M. shangchengensis and T. ampliceps and the comparative analyses showed that the gut microbial communities varied according to the phylogeny and the diet habits of termites.

  18. Plant-mediated synthesis of highly active iron nanoparticles for Cr (VI) removal: Investigation of the leading biomolecules.

    PubMed

    Xiao, Zhengli; Yuan, Min; Yang, Bin; Liu, Zhaoyan; Huang, Jiale; Sun, Daohua

    2016-05-01

    The eco-friendly synthesis and application of Fe nanoparticles (Fe NPs) with higher activity and stability has aroused wide attention in the field of pollutant remediation. In this work, 15 plants extracts were selected for the plant-mediated synthesis of Fe NPs. The as-synthesized particles' morphology and structure were characterized by transmission electron microscopy, X-ray diffraction and UV-Vis spectroscopy. The contents of four main active biomolecules in the 15 extracts were determined, and comparative studies were further carried out to clarify the key component closely related to the reducing capacity. The results demonstrate that polyphenol is the leading ingredient involved in the biosynthesis of Fe NPs. Then Fe products synthesized by three extracts with distinct content of polyphenol were employed to remove Cr (VI) in the aqueous solution, indicating that the activity of the Fe NPs for Cr (VI) removal is consistent with the reducing capacity of the extracts. Furthermore, the Fe NPs synthesized by S. jambos(L.) Alston extract (SJA-Fe NPs) showed significant removal capacity of Cr(VI) with 698.6 mg Cr(VI) per g of iron. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Alteration of liver glycopatterns during cirrhosis and tumor progression induced by HBV.

    PubMed

    Qin, Yannan; Zhong, Yaogang; Ma, Tianran; Wu, Fei; Wu, Haoxiang; Yu, Hanjie; Huang, Chen; Li, Zheng

    2016-04-01

    The incidence of hepatocellular carcinoma (HCC) is closely correlated with hepatitis B virus (HBV)-induced liver cirrhosis. Structural changes in the glycans of serum and tissue proteins are reliable indicators of liver damage. However, little is known about the alteration of liver glycopatterns during cirrhosis and tumor progression induced by HBV infection. This study compared the differential expression of liver glycopatterns in 7 sets of normal pericarcinomatous tissues (PCTs), cirrhotic, and tumor tissues from patients with liver cirrhosis and HCC induced by HBV using lectin microarrays. Fluorescence-based lectin histochemistry and lectin blotting were further utilized to validate and assess the expression and distribution of certain glycans in 9 sets of corresponding liver tissue sections. Eight lectins (e.g., Jacalin and AAL) revealed significant difference in cirrhotic tissues versus PCTs. Eleven lectins (e.g., EEL and SJA) showed significant alteration during cirrhotic and tumor progression. The expression of Galα1-3(Fucα1-2)Gal (EEL) and fucosyltransferase 1 was mainly increasing in the cytoplasm of hepatocytes during PCTs-cirrhotic-tumor tissues progression, while the expression of T antigen (ACA and PNA) was decreased sharply in cytoplasm of tumor hepatocytes. Understanding the precision alteration of liver glycopatterns related to the development of hepatitis, cirrhosis, and tumor induced by HBV infection may help elucidate the molecular mechanisms underlying the progression of chronic liver diseases and develop new antineoplastic therapeutic strategies.

  20. Community Assembly and Ecology of Activated Sludge Under Photosynthetic Feast/Famine Conditions.

    PubMed

    Oyserman, Ben O; Martirano, Joseph M; Wipperfurth, Spenser; Owen, Brian R; Noguera, Daniel R; McMahon, Katherine D

    2017-02-27

    Here we demonstrate that photosynthetic oxygen production under light/dark, feast/famine cycles with no mechanical aeration and negligible oxygen diffusion is able to maintain phosphorus cycling activity associated with the enrichment of polyphosphate accumulating organisms (PAOs). We investigate the ecology of this novel system by conducting a time series analysis of prokaryotic and eukaryotic biodiversity using the V3-V4 and V4 region of the 16S and 18S rRNA gene sequences, respectively. In the Eukaryotic community, the initial dominant alga observed was Desmodesmus. During operation, the algal community became a more diverse consortium of Desmodesmus, Parachlorella, Characiopodium and Bacillariophytina. In the Prokaryotic community, there was an initial enrichment of the PAO Candidatus Accumulibacter phosphatis (Accumulibacter) Acc-SG2, and the dominant ammonia-oxidizing organism was Nitrosomonas oligotropha, however these populations decreased in relative abundance, becoming dominated by Accumulibacter Acc-SG3 and Nitrosomonas ureae. Furthermore, functional guilds that were not abundant initially became enriched including the putative Cyanobacterial PAOs Obscuribacterales and Leptolyngbya, and the H2-oxidizing denitrifying autotroph Sulfuritalea. After a month of operation, the most abundant prokaryote belonged to an uncharacterized clade of Chlorobi classified as Chlorobiales;SJA-28 Clade III, the first reported enrichment of this lineage. This experiment represents the first investigation into the ecological interactions and community assembly during photosynthetic feast/famine conditions. Our findings suggest that photosynthesis may provide sufficient oxygen to drive polyphosphate cycling.

  1. Genetic control of BCG-induced granulomatous inflammation in mice.

    PubMed

    Sternick, J L; Schrier, D J; Moore, V L

    1983-11-01

    The genetics of BCG-induced pulmonary granulomatous inflammation (PGI) and splenomegaly was studied by breeding experiments and by the use of BXD recombinant inbred (RI) and allotype-congenic mice. Breeding studies indicated that the genetic control was multifactorial; this observation was confirmed using BXD RI mice. In addition, studies with BXD RI mice suggested that genes linked to the Igh complex influence responsiveness. The influence of the Igh-linked genes was studied further using allotype-congenic mice; SJL mice (Ighb) developed significantly greater PGI than their congenic partner, SJA/9 (Igha). Data from BALB.Igb, CB-20, and BAB-14 mice suggested that Igh-linked genes influencing PGI were a considerable distance from Igh-1. Igh-linked genes that influence BCG-induced splenomegaly were located on the centrometric side of the Igh-1 locus. This was shown by data in which splenomegaly in BALB.Igb and CB-20, but not BAB-14, mice was significantly augmented over BALB/c mice. Further studies are necessary to understand the significance of these observations.

  2. Inhibition of Pasteurella multocida Adhesion to Rabbit Respiratory Epithelium Using Lectins

    PubMed Central

    Carrillo, Magda Patricia; Martinez, Nhora María; Patiño, María del Pilar; Iregui, Carlos Arturo

    2015-01-01

    This study aimed to evaluate the ability of a panel of lectins to inhibit the ability of Pasteurella multocida to adhere to and affect the rabbit respiratory epithelium. Nasal septa from rabbit fetuses were cultured with various lectins before the addition of P. multocida. The percentage of bacteria adhering to the epithelium was evaluated semiquantitatively by indirect immunoperoxidase (IIP) staining. The goblet cells (GCs) were counted in semithin sections stained with toluidine blue and served as the main morphological criterion to evaluate the inhibitory effect of the lectins. The lectins PNA, WGA, RCA120, and DBA significantly inhibited the adhesion of P. multocida to the ciliated epithelium (P < 0.05) and prevented the pathogen-induced increase in the number of GCs (P < 0.05) compared with those of positive control tissues. In addition, VVA, SJA, UEA I, DSL, SBA, and ECL significantly inhibited the increase in GCs compared with that of the control tissues. The results suggest that less aggressive therapeutic strategies, such as treatment with lectins, may represent alternative approaches to control bacterial respiratory infections. PMID:25810949

  3. Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus

    PubMed Central

    FERRARI, C. C.; CARMANCHAHI, P. D.; ALDANA MARCOS, H. J.; MUGNAINI, M. T.; AFFANNI, J. M.; PAZ, D. A.

    1999-01-01

    Conventional histochemistry and the binding patterns of 22 biotinylated lectins were examined for characterisation of glycoconjugates in the components of the olfactory mucosa of the armadillo Chaetophractus villosus. The mucous lining the olfactory epithelium showed binding sites for DSL, WGA, STL, LEL, PHA-E and JAC. Only the basilar processes of the supporting cells stained for Con-A and S-Con A. The olfactory receptor neurons stained with LEL, LCA, Con A, S-Con A, JAC and PNA. The layer of basal cells did not react with any of the lectins studied. Bowman's glands in the lamina propria showed subpopulations of acinar cells reacting with SBA, S-WGA, WGA, STL, Con A, PSA, PNA, SJA, VVA, JAC and S-Con A, but in our optical studies with lectins we were unable to differentiate between mucous and serous cells in the way that is possible on electron microscopy. The ducts of Bowman's glands were labelled with S-WGA, STL, LEL, PHA-E, BSL-I and JAC. This histochemical study on the glycoconjugates of the olfactory mucosa in the order Xenarthra provides a basis for further experimental investigations. PMID:10386777

  4. Variation in the saccharide lectin binding pattern from different isolates of Tritrichomonas foetus.

    PubMed

    Doumecq, María Laura; Soto, Pedro; Casalini, María Belén; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Monteavaro, Cristina Esther

    2014-12-01

    Tritrichomonas foetus (T. foetus) is the causal agent of bovine tritrichomonosis (BT), a venereal disease that causes significant economic losses in the bovine livestock industry. The structural organization of T. foetus presents a cell membrane, an undulating membrane which extends along the parasite, three anterior flagella and a recurrent posterior flagellum. The interaction between the superficial glycoconjugates of the parasite and the host cell is one of the most relevant pathogenic mechanisms. In the present study, we analyzed the saccharide pattern through lectincytochemistry of the cell membrane, undulating membrane, cytoplasm and flagella of 28 isolates of T. foetus. Lectins that labeled most of the isolates were WGA, Con-A, RCA-I, LCA, GS-II and PHA-E showing the presence of D-mannose, D-glucose, N-acetylglucosamine and sialic acid. On the other hand, no labeling was observed in any of the structures with VVA, STA, LEA, Jacalin, GS-I, SJA, PHA-L, DSA, and weak labeling was observed with DBA, PNA, SBA and UEA I, showing therefore a low expression of N-acetylgalactosamine, L-fucose and galactose. In addition, GS II labeled in a granular pattern when lectincytochemistry was positive, whereas LCA strongly labeled the membranes and weakly the cytoplasms. The labeling variations observed among the isolates analyzed in the present work, could be related to differences in the pathogenic behavior of the isolates.

  5. Inhibition of calpain attenuates encephalitogenicity of MBP-specific T cells

    PubMed Central

    Guyton, Mary K.; Das, Arabinda; Inoue, Jun; Azuma, Mitsuyoshi; Ray, Swapan K.; Brahmachari, Saurav; Banik, Naren L.

    2009-01-01

    Multiple sclerosis (MS) is a T cell-mediated autoimmune disease of the CNS, possessing both immune and neurodegenerative events that lead to disability. Adoptive transfer (AT) of myelin basic protein (MBP)-specific T cells into naïve female SJL/J mice results in a relapsing-remitting (RR) form of experimental autoimmune encephalomyelitis (EAE). Blocking the mechanisms by which MBP-specific T cells are activated before AT may help characterize the immune arm of MS and offer novel targets for therapy. One such target is calpain, which is involved in activation of T cells, migration of immune cells into the CNS, degradation of axonal and myelin proteins, and neuronal apoptosis. Thus, the hypothesis that inhibiting calpain in MBP-specific T cells would diminish their encephalitogenicity in RR-EAE mice was tested. Incubating MBP-specific T cells with the calpain inhibitor SJA6017 before AT markedly suppressed the ability of these T cells to induce clinical symptoms of RR-EAE. These reductions correlated with decreases in demyelination, inflammation, axonal damage, and loss of oligodendrocytes and neurons. Also, calpain:calpastatin ratio, production of tBid, and Bax:Bcl-2 ratio, and activities of calpain and caspases, and internucleosomal DNA fragmentation were attenuated. Thus, these data suggest calpain as a promising target for treating EAE and MS. PMID:19627443

  6. Western equatorial African forest-savanna mosaics: a legacy of late Holocene climatic change?

    NASA Astrophysics Data System (ADS)

    Ngomanda, A.; Chepstow-Lusty, A.; Makaya, M.; Favier, C.; Schevin, P.; Maley, J.; Fontugne, M.; Oslisly, R.; Jolly, D.

    2009-10-01

    Past vegetation and climate changes reconstructed using two pollen records from Lakes Maridor and Nguène, located in the coastal savannas and inland rainforest of Gabon, respectively, provide new insights into the environmental history of western equatorial African rainforests during the last 4500 cal yr BP. These pollen records indicate that the coastal savannas of western equatorial Africa did not exist during the mid-Holocene and instead the region was covered by evergreen rainforests. From ca. 4000 cal yr BP a progressive decline of inland evergreen rainforest, accompanied by the expansion of semi-deciduous rainforest, occurred synchronously with grassland colonisation in the coastal region of Gabon. The contraction of moist evergreen rainforest and the establishment of coastal savannas in Gabon suggest decreasing humidity from ca. 4000 cal yr BP. The marked reduction in evergreen rainforest and subsequent savanna expansion was followed from 2700 cal yr BP by the colonization of secondary forests dominated by the palm, Elaeis guineensis, and the shrub, Alchornea cordifolia (Euphorbiaceae). A return to wetter climatic conditions from about 1400 cal yr BP led to the renewed spread of evergreen rainforest inland, whereas a forest-savanna mosaic still persists in the coastal region. There is no evidence to suggest that the major environmental changes observed were driven by human impact.

  7. The DYNAFLUX / DYNACOLD (Dynamics, Fluxes, Stability, Succession and Landscape Formation in Cold Environments) Network (2004-2017)

    NASA Astrophysics Data System (ADS)

    Beylich, Achim A.

    2017-04-01

    There is a wide range of high-latitude and high-altitude cold climate landscapes within Europe, covering a significant proportion of the total land surface area. This spectrum of defined cold-climate landscapes represents a variety of stages of deglaciation history and landscape formation. We can find landscapes at different levels of postglacial stabilization which is providing the unique opportunity to study the interactions between geo-, bio-, social and socio-economic systems at the land surface. The DYNAFLUX / DYNACOLD Network (2004-2017) bridges across the geo-, bio-, social and socio-economic sciences in order to analyze the complex dynamics of adjustment, stabilization, succession and landscape formation during and after ice retreat and under ongoing anthropogenic influences. The network provides a multidisciplinary forum where researchers come together and discuss. In addition, this network is linking a number of other scientific networks, working groups and programs and creates an umbrella network and a forum for sharing knowledge and experience. The scientific focus of DYNAFLUX / DYNACOLD is also relevant for a number of end users, including risk and vulnerability assessment, sustainable land use, land management and conservation. In addition, present key questions related to environmental change like, e.g., hazards, permafrost degradation and loss of biodiversity are addressed and discussed. Further information is found under http://www.ngu.no/sediflux.

  8. Automated paleomagnetic and rock magnetic data acquisition with an in-line horizontal "2G" system

    NASA Astrophysics Data System (ADS)

    Mullender, Tom A. T.; Frederichs, Thomas; Hilgenfeldt, Christian; de Groot, Lennart V.; Fabian, Karl; Dekkers, Mark J.

    2016-09-01

    Today's paleomagnetic and magnetic proxy studies involve processing of large sample collections while simultaneously demanding high quality data and high reproducibility. Here we describe a fully automated interface based on a commercial horizontal pass-through "2G" DC-SQUID magnetometer. This system is operational at the universities of Bremen (Germany) and Utrecht (Netherlands) since 1998 and 2006, respectively, while a system is currently being built at NGU Trondheim (Norway). The magnetometers are equipped with "in-line" alternating field (AF) demagnetization, a direct-current bias field coil along the coaxial AF demagnetization coil for the acquisition of anhysteretic remanent magnetization (ARM) and a long pulse-field coil for the acquisition of isothermal remanent magnetization (IRM). Samples are contained in dedicated low magnetization perspex holders that are manipulated by a pneumatic pick-and-place-unit. Upon desire samples can be measured in several positions considerably enhancing data quality in particular for magnetically weak samples. In the Bremen system, the peak of the IRM pulse fields is actively measured which reduces the discrepancy between the set field and the field that is actually applied. Techniques for quantifying and removing gyroremanent overprints and for measuring the viscosity of IRM further extend the range of applications of the system. Typically c. 300 paleomagnetic samples can be AF demagnetized per week (15 levels) in the three-position protocol. The versatility of the system is illustrated by several examples of paleomagnetic and rock magnetic data processing.

  9. Populations of domesticated cattle and buffalo in the Western Forest Complex of Thailand and their possible impacts on the wildlife community.

    PubMed

    Chaiyarat, Rattanawat; Srikosamatara, Sompod

    2009-03-01

    The Western Forest Complex (WEFCOM) of Thailand is comprised of many protected areas and has one of the highest wildlife populations in the country. Populations of wildlife in the WEFCOM have decreased dramatically over recent years. Rapid economic development has resulted in the conversion of forest into agricultural and pastoral land, which has directly and indirectly impacted the wildlife community. This research aimed to evaluate populations of domesticated cattle (Bos indicus) and buffalo (Bubalus bubalis) in the WEFCOM and their possible impacts on the wildlife community. Domesticated cattle and buffalo keepers from 1561 (or 3.3%) of houses in and near WEFCOM were interviewed. The average number of animals per household was 15.6 cattle and 8.5 buffalo. Most villagers released domesticated cattle and buffalo to forage in the protected areas. This tended to have a high impact on the wildlife community in Huai Kha Khaeng Wildlife Sanctuary and Tungyai Naresuan Wildlife Sanctuary. The least impacted areas were Luam Khlong Ngu National Park, Thong Pha Phum National Park and Chaleam Ratanakosin National Park. With a high risk to the wildlife community, law enforcement should be used in combination with a certain level of co-management with local communities.

  10. AB38. Microorganisms in Chronic prostatitis in outpatient clinic Mongolia

    PubMed Central

    Samdankhuu, Khongorzul; Sanjmyatav, Purevjal; Damiran, Naransukh; Naidan, Nansalmaa

    2014-01-01

    Background Recent years, morbidity of chronic prostatis is increasing in Mongolia. Most common cause of the chronic prostatis is Non-Gonococcal Urethritis (NGU) such as chlamydia trachomatis, mycoplasma hominis, mycoplasma genitalium, ureaplasma urealyticum and ureaplasma parvum or mixed infections. Purpose The purpose of the study was to research possible relationships between signs or symptoms of the chronic prostatitis and its cause. Method A total of 466 males who have possible signs of chronic prostatitis were enrolled in the study. All patients were checked for urinalysis and expressed prostate secretion (EPS) and Polymerase Chain Reaction (PCR) of EPS detection for neisser a gonorrhea, chlamydia trachomatis, mycoplasma hominis, mycoplasma genitalium, ureaplasma urealyticum and ureaplasma parvum at an outpatient center of andrology office, Ulaanbaatar, Mongolia. Results Two hundred and eighty three of all participants have positive PCR results. Mean age of in our study was 33.1±8 years. U. realyticum was the most common (48.6%), followed by M. hominis and C. trachomatis mixed infection. In Mach, April and August were highest episodes of morbidity were registered 16.2%, 11.69% and 10.71%. Chronic pelvic pain syndrome and lower urinary tract symptoms were the most common complaints of main reason to visit outpatient clinic. Conclusions U. urealyticum was most common infection detected by PCR analyses among men who have chronic prostatitis, who have chronic pelvic pain syndrome. Further research is needed to determine if there are associations between signs or symptoms and cause of the chronic prostatis.

  11. Susceptibility of mixed infection of Ureaplasma Urealyticum and Mycoplasma Hominis to seven antimicrobial agents and comparison with that of Ureaplasma Urealyticum infection.

    PubMed

    Huang, Changzheng; Liu, Zhixiang; Lin, Nengxing; Tu, Yating; Li, Jiawen; Zhang, Demei

    2003-01-01

    In order to investigate the susceptibility of mixed infection of Ureaplasma Urealyticum (UU) and Mycoplasma Hominis (MH) to 7 kinds of antimicrobial agents and comparison with that of UU infection in NGU patients, the in vitro susceptibility was determined by using microdilution method. The positive results were analyzed. The results showed that the sequence of susceptibility to 7 kinds of antimicrobial agents for both UU infection group and UU-MH mixed infection group was almost the same from the highest susceptibility to the lowest accordingly: Josamycin, Doxycycline, Minocycline, Sparfloxacin, Roxithromycin, Ofloxacin and Azithromycin. The total drug resistance rate for UU-MH mixed infection group (97.67%) was significantly higher than that for UU infection group (44.67%, P < 0.01). The highest drug resistance rate in UU group and UU-MH mixed infection group was 31.33% (Ofloxacin) and 90.48% (Azithromycin) respectively. UU-MH mixed infection showed an increased drug resistance and changes of drug resistance spectrum.

  12. Sexually transmitted diseases in Ulaanbaatar, Mongolia

    PubMed Central

    Schwebke, J R; Aira, T; Jordan, N; Jolly, P E; Vermund, S H

    2009-01-01

    Summary Although human immunodeficiency virus (HIV) rates are increasing rapidly in Asia, a full understanding of the extent of other sexually transmitted diseases (STDs) in many of these areas is lacking. There have been anecdotes of rising rates of STDs in Mongolia, a country thus far relatively unaffected by HIV. To further the understanding of STDs, a prevalence study was conducted in the STD clinic in Ulaanbaatar, the capital and largest city in Mongolia. Among 260 patients, the prevalence of gonorrhoea, chlamydia and syphilis was 31.1%, 8.1% and 8.6% respectively for males and 10.3%, 9.9% and 6.0% for females. Sixty-seven per cent of females had trichomoniasis and 19.7% of males had non-gonococcal urethritis (NGU). Forty-two per cent of gonococcal isolates had plasmid mediated resistance to penicillin, and chromosomal resistance to penicillin, tetracycline, and ciprofloxacin was documented. No patients were infected with HIV. STDs are a significant problem in Mongolia. Improved control efforts are urgently needed to prevent the emergence of HIV. PMID:9671251

  13. Vortex Ring Formation Characteristics in Synthetic Jet due to Changes of Excitation Frequency in the ½-Ball Cavity Actuator

    NASA Astrophysics Data System (ADS)

    Kosasih, Engkos A.; Harinaldi; Trisno, Ramon

    2017-04-01

    A jet flow that contains vortex ring has a large energy compared to a regular jet. As one of the causes of the aerodynamic drag to the vehicle, the flow separation that occurs behind the bluff body must be controlled, so that aerodynamic drag can be significantly reduced. This study is a basic work on the development of turbulent flow separation control for aerodynamic purpose, especially in the design of the vehicle body. The main objective of this study is to analyze the performance of the synthetic jet (SJA) as one of flow control tool to reduce separation area. To get the maximum performance of the synthetic jet actuator, the research starts by characterizing the actuator. Characterization of ½ ball-shaped cavity is done with excitation frequency changes and orifice diameter of 3, 5 and 8 mm. The study was conducted using computational and experimental methods. The experimental data was obtained by testing synthetic jet actuator with providing sinusoidal signal to drive the membrane and at the orifice end a hotwire probe that is set and plugged into a CTA (Constant Temperature Anemometry) to obtain the speed velocity of the exhaust jet. Computational methods used a commercial CFD software (FLUENT 6.3) with a Reynolds Stress Model as a model of turbulence. Each of these calculations or measurements was conducted under the same conditions. The research result is displayed in frequency testing curve to get the maximum velocity of the jet stream. The results are further indicative of the synthetic jet actuator capability to generate vortex rings. In the experimental results, the determination of ring vortex formation taken from the calculation of the flow velocity, while the CFD simulations, the formation of vortex rings can be seen from the visualization of the flow contour. Vortex ring formed from this ½ -ball cavity, occurred at 3 mm and 5 mm orifice diameter, while the 8 mm orifice diameter cavity cannot form a ring vortex.

  14. Calpain- and caspase-mediated alphaII-spectrin and tau proteolysis in rat cerebrocortical neuronal cultures after ecstasy or methamphetamine exposure.

    PubMed

    Warren, Matthew W; Zheng, Wenrong; Kobeissy, Firas H; Cheng Liu, Ming; Hayes, Ronald L; Gold, Mark S; Larner, Stephen F; Wang, Kevin K W

    2007-08-01

    Abuse of 3,4-methylenedioxymethamphetamine (MDMA or Ecstasy) and methamphetamine (Meth or Speed) is a growing international problem with an estimated 250 million users of psychoactive drugs worldwide. It is important to demonstrate and understand the mechanism of neurotoxicity so potential prevention and treatment therapies can be designed. In this study rat primary cerebrocortical neuron cultures were challenged with MDMA and Meth (1 or 2 mM) for 24 and 48 h and compared to the excitotoxin N-methyl-D-aspartate (NMDA). The neurotoxicity of these drugs, as assessed by microscopy, lactate dehydrogenase release and immunoblot, was shown to be both dose- and time-dependent. Immunoblot analysis using biomarkers of cell death showed significant proteolysis of both alphaII-spectrin and tau proteins. Breakdown products of alphaII-spectrin (SBDPs) of 150, 145, and 120 kDa and tau breakdown products (TBDPs) of 45, 32, 26, and 14 kDa were observed. The use of the protease inhibitors calpain inhibitor SJA6017 and caspase inhibitors z-VAD-fmk and Z-D-DCB, attenuated drug-induced alphaII-spectrin and tau proteolysis. The calpain inhibitor reduced the calpain-induced breakdown products SBDP145 and TBDP14, but there was an offset increase in the caspase-mediated breakdown products SBDP120 and TBDP45. The caspase inhibitors, on the other hand, decreased SBDP120 and TBDP45. These data suggest that both MDMA and Meth trigger concerted proteolytic attacks of the structural proteins by both calpain and caspase family of proteases. The ability of the protease inhibitors to reduce the damage caused by these drugs suggests that the treatment arsenal could include similar drugs as possible tools to combat the drug-induced neurotoxicity in vivo.

  15. Substrate-Driven Convergence of the Microbial Community in Lignocellulose-Amended Enrichments of Gut Microflora from the Canadian Beaver (Castor canadensis) and North American Moose (Alces americanus).

    PubMed

    Wong, Mabel T; Wang, Weijun; Lacourt, Michael; Couturier, Marie; Edwards, Elizabeth A; Master, Emma R

    2016-01-01

    Strategic enrichment of microcosms derived from wood foragers can facilitate the discovery of key microbes that produce enzymes for the bioconversion of plant fiber (i.e., lignocellulose) into valuable chemicals and energy. In this study, lignocellulose-degrading microorganisms from the digestive systems of Canadian beaver (Castor canadensis) and North American moose (Alces americanus) were enriched under methanogenic conditions for over 3 years using various wood-derived substrates, including (i) cellulose (C), (ii) cellulose + lignosulphonate (CL), (iii) cellulose + tannic acid (CT), and (iv) poplar hydrolysate (PH). Substantial improvement in the conversion of amended organic substrates into biogas was observed in both beaver dropping and moose rumen enrichment cultures over the enrichment phases (up to 0.36-0.68 ml biogas/mg COD added), except for enrichments amended with tannic acid where conversion was approximately 0.15 ml biogas/mg COD added. Multiplex-pyrosequencing of 16S rRNA genes revealed systematic shifts in the population of Firmicutes, Bacteroidetes, Chlorobi, Spirochaetes, Chloroflexi, and Elusimicrobia in response to the enrichment. These shifts were predominantly substrate driven, not inoculum driven, as revealed by both UPGMA clustering pattern and OTU distribution. Additionally, the relative abundance of multiple OTUs from poorly defined taxonomic lineages increased from less than 1% to 25-50% in microcosms amended with lignocellulosic substrates, including OTUs from classes SJA-28, Endomicrobia, orders Bacteroidales, OPB54, and family Lachnospiraceae. This study provides the first direct comparison of shifts in microbial communities that occurred in different environmental samples in response to multiple relevant lignocellulosic carbon sources, and demonstrates the potential of enrichment to increase the abundance of key lignocellulolytic microorganisms and encoded activities.

  16. Substrate-Driven Convergence of the Microbial Community in Lignocellulose-Amended Enrichments of Gut Microflora from the Canadian Beaver (Castor canadensis) and North American Moose (Alces americanus)

    PubMed Central

    Wong, Mabel T.; Wang, Weijun; Lacourt, Michael; Couturier, Marie; Edwards, Elizabeth A.; Master, Emma R.

    2016-01-01

    Strategic enrichment of microcosms derived from wood foragers can facilitate the discovery of key microbes that produce enzymes for the bioconversion of plant fiber (i.e., lignocellulose) into valuable chemicals and energy. In this study, lignocellulose-degrading microorganisms from the digestive systems of Canadian beaver (Castor canadensis) and North American moose (Alces americanus) were enriched under methanogenic conditions for over 3 years using various wood-derived substrates, including (i) cellulose (C), (ii) cellulose + lignosulphonate (CL), (iii) cellulose + tannic acid (CT), and (iv) poplar hydrolysate (PH). Substantial improvement in the conversion of amended organic substrates into biogas was observed in both beaver dropping and moose rumen enrichment cultures over the enrichment phases (up to 0.36–0.68 ml biogas/mg COD added), except for enrichments amended with tannic acid where conversion was approximately 0.15 ml biogas/mg COD added. Multiplex-pyrosequencing of 16S rRNA genes revealed systematic shifts in the population of Firmicutes, Bacteroidetes, Chlorobi, Spirochaetes, Chloroflexi, and Elusimicrobia in response to the enrichment. These shifts were predominantly substrate driven, not inoculum driven, as revealed by both UPGMA clustering pattern and OTU distribution. Additionally, the relative abundance of multiple OTUs from poorly defined taxonomic lineages increased from less than 1% to 25–50% in microcosms amended with lignocellulosic substrates, including OTUs from classes SJA-28, Endomicrobia, orders Bacteroidales, OPB54, and family Lachnospiraceae. This study provides the first direct comparison of shifts in microbial communities that occurred in different environmental samples in response to multiple relevant lignocellulosic carbon sources, and demonstrates the potential of enrichment to increase the abundance of key lignocellulolytic microorganisms and encoded activities. PMID:27446004

  17. Quantitative Analysis of the Spectrum of the B3 Abundance Standard iota Herculis

    NASA Astrophysics Data System (ADS)

    Peters, Geraldine J.; Adelman, S. J.; Gulliver, A.

    2010-01-01

    Iota Herculis is an ultrasharp-lined B3IV star that historically has been considered as an abundance standard for the early B stars. New abundances of the lighter and Fe group elements have been determined with the aid of high resolution spectral data from HST/STIS and the Dominion Astrophysical Observatory. The HST spectra were obtained with the STIS E140M and E230M gratings and cover the region 1160-2350 A with a 2-pixel spectral resolution that varies from 0.03-0.08 A. The DAO spectral atlas covers 3820-5350 A in the second order with a 2-pixel resolution 0.072 A. We compare theoretical spectra computed from the NLTE code TLUSTY/SYNSPEC (I. Hubeny & T. Lanz, ApJ, 439,875,1995) and the LTE code ATLAS9/SYNTHE (R. Kurucz, 1993, CD-ROMs 13 & 18) with the observations. The model parameters adopted for the star are Teff = 17,500 K, log g = 3.75, Vturb = 0 km/s, and vsin i = 5 km/s. Solar abundances appear to prevail for the lighter elements but the abundances of Fe group elements are 0.1-0.3 dex below the solar values given by Asplund, Grevesse, & Sauval (2005). In general the NLTE calculations fit the observations better, especially for lines formed high in the atmosphere such as the C I lines near 1260 A. The detailed analysis of the spectrum of this standard star not only allows one to determine the abundances in the atmosphere of a typical field B star but also presents the opportunity to assess the accuracy of atomic parameters for UV lines and find astrophysical f-values. The authors appreciate support from NASA and STScI grants NAG5-11802 and GO-09848. SJA was a guest observer at DAO.

  18. Processing of cdk5 activator p35 to its truncated form (p25) by calpain in acutely injured neuronal cells.

    PubMed

    Nath, R; Davis, M; Probert, A W; Kupina, N C; Ren, X; Schielke, G P; Wang, K K

    2000-07-21

    Recently, it was shown that conversion of cdk5 activator protein p35 to a C-terminal fragment p25 promotes a deregulation of cdk5 activity, which may contribute to neurodegeneration in Alzheimer's disease. In this study, we present evidence that calpain is a protease involved in the conversion of p35 to p25. To activate calpain, rat cerebellar granule neurons were treated with maitotoxin (MTX). A C-terminus-directed anti-p35 antibody detected that p35 conversion to p25 paralleled the formation of calpain-generated alpha-spectrin (alpha-fodrin) breakdown products (SBDP's) in a maitotoxin-dose-dependent manner. Two calpain inhibitors (MDl28170 and SJA6017) reduced p35 processing but were unchanged when exposed to the caspase inhibitor carbobenzoxy-Asp-CH(2)OC(=O)-2, 6-dichlorobenzene or the proteasome inhibitors (lactacystin and Z-Ile-Glu(OtBu)Ala-Leu-CHO). p35 protein was also degraded to p25 when rat brain lysate was subjected to in vitro digestion with purified mu- and m-calpains. Additionally, in a rat temporary middle cerebral artery occlusion model, p35 processing to p25 again paralleled SBDP formation in the ischemic core. Lastly, in malonate-injured rat brains, the ipsilateral side showed a striking correlation of SBDP formation with p35 to p25 conversion and tau phosphorylation (at Ser202 and Thr205) increase. These data suggest that calpain is a major neuronal protease capable of converting p35 to p25 and might play a pathological role of activating cdk5 and its phosphorylation of tau in Alzheimer's disease.

  19. Comparative Gut Microbiomes of Four Species Representing the Higher and the Lower Termites

    PubMed Central

    Su, LiJuan; Yang, LeLe; Huang, Shi; Su, XiaoQuan; Li, Yan; Wang, FengQin; Wang, EnTao; Kang, Ning; Xu, Jian; Song, AnDong

    2016-01-01

    Aiming at learning the association between the gut microbiota and termites with different diet habits and phylogenetic positions, the gut bacteria of three populations for each of the two higher termites (wood-feeding Mironasutitermes shangchengensis and fungus-feeding Odontotermes formosanus) and two wood-feeding lower termites (Tsaitermes ampliceps and Reticulitermes flaviceps) were analyzed by high-throughput 454 pyrosequencing of 16S V1–V3 amplicons. As results, 132 bacterial genera and some unidentified operational taxonomic units within 29 phyla in the gut bacteria were detected, with Spirochaetes (11–55%), Firmicutes (7–18%), Bacteroidetes (7–31%), and Proteobacteria (8–14%) as the main phyla, and Treponema, TG5, Dysgonomonas, Tannerella, za29, Lactococcus, Pseudomonas, and SJA-88 as the common genera in all the four termites. The diversity of gut bacterial communities in the higher termite guts was significantly greater than that in the lower termites; while the gut microbiota in M. shangchengensis (wood-feeding higher termite) was more similar to those of the wood-feeding lower termites rather than that of O. formosanus (fungus-feeding higher termite), and phylum Spirochaetes and nitrogen-fixing bacteria were super-dominant in the wood-feeding termites, despite of their phylogenetic relations. This study reported for the first time the gut bacterial communities for the termites of M. shangchengensis and T. ampliceps and the comparative analyses showed that the gut microbial communities varied according to the phylogeny and the diet habits of termites. PMID:27638955

  20. Heterogeneity of Igh-linked allotypic determinants expressed on functional T cell subsets as detected by monoclonal antibodies.

    PubMed

    Karasuyama, H; Kim, M; Okumura, K; Tada, T

    1984-05-01

    Hybridomas producing monoclonal antibodies (mAb) specific for immunoglobulin heavy chain (Igh) allotype-linked gene products expressed only on functional T cells but not on B cells and macrophages were established by fusion of allotype congenic SJL (Igh-1b) and SJA /9 (Igh-1a) B cells immunized reciprocally with partner spleen cells with a myeloma P3-X63-Ag8-653 of BALB/c origin. Nine mAb have been selected on the criteria that they can specifically block various antigen-dependent functions of known T cell subsets in in vitro immune responses of mouse strains having the corresponding Igh allotype, but not the other one. These included (a) four mAb that augment the in vitro secondary antibody response of either Igh-1a or Igh-1b strains and thus are considered to react with the Igh-linked allotypic determinant expressed on suppressor T cells, (b) one mAb that inhibits the helper T cell activity of Igh-1b but not of Igh-1a strains, (c) two mAb that inhibit the antigen-induced proliferative response of Igh-1a but not Igh-1b strains, and (d) two mAb that block the cytotoxicity of alloreactive cytotoxic T cells of Igh-1a strains. The linkage to Igh-1 allotype of the T cell products was established by testing with Igh-1-congenic strains with different backgrounds including the H-2 complex. Some of the mAb were able to react with cloned hybridomas and a continuous cell line of the given allotype and functions. Each mAb was able to block one of the known functions of T cell subsets, but not others, indicating the existence of the heterogeneity and multiplicity of the Igh allotype-linked products on T cells.

  1. Peculiarities of internal radiation doses due to ¹³⁷Cs and ⁹⁰Sr intake in population from Zhytomyr oblast in a late period after the Chornobyl NPP accident.

    PubMed

    Vasylenko, V V; Tsigankov, M Y; Nechaev, S Y; Pikta, V O; Zadorozhna, G M; Bilonyk, A B

    2013-01-01

    Meta doslidzhennja – vyznachennja osoblyvostej formuvannja doz vnutrishn'ogo oprominennja meshkanciv Zhytomyrs'koi' oblasti ta naukove obgruntuvannja zahodiv shhodo zmenshennja doz vnutrishn'ogo oprominennja naselennja radioaktyvno zabrudnenyh terytorij u viddalenyj period avarii' na ChAES. Materialy i metody. Bulo provedeno kompleksnyj radiacijnyj monitoryng u 4 naselenyh punktah Narodyc'kogo rajonu Zhytomyrs'koi' oblasti. Dlja doslidzhen' buly zastosovani metody – dozymetrychni (instrumental'ni ta rozrahunkovi), radiohimichni. Rezul'taty i vysnovky. Provedenyj LVL-monitoryng u 2012 roci u Narodyc'komu rajoni Zhytomyrs'koi' oblasti zarejestruvav znachne u 2–3,5 raza sezonne pidvyshhennja rivniv inkorporacii' 137Cs v organizmi meshkanciv obstezhenyh naselenyh punktiv, jak u doroslyh, tak i u ditej. Majzhe u 5 % osib z chysla obstezhenyh vstanovleno perevyshhennja dozovogo rivnja 1,0 mZv x rik-1. Maksymal'ne znachennja vmistu inkorporovanogo 137Cs, zarejestrovane u cyh naselenyh punktah, stanovyt' 350 kBk, shho formuje richnu dozu vnutrishn'ogo oprominennja 10 mZv x rik-1. Vmist 137Cs u probah moloka v obstezhenyh gospodarstvah vprodovzh roku malo zminyvsja – u 30–50 % prob moloka u smt Narodychi ta s. Selec' zarejestrovano perevyshhennja dopustymogo rivnja vmistu 137Cs – 100 Bk x l-1, u selah Motijky ta Hrystynivka vmist 137Cs u probah moloka ne perevyshhuje dopustymyh rivniv. Vmist 90Sr jak u moloci, tak i v kartopli, znachno nyzhchyj dopustymogo rivnja – 20 Bk x l-1. Vmist 137Cs u kartopli ne perevyshhuje dopustymi rivni. Vstanovleno, shho osnovna chastka dozy vnutrishn'ogo oprominennja meshkanciv obstezhenyh naselenyh punktiv Zhytomyrs'koi' oblasti formujet'sja za rahunok spozhyvannja miscevyh produktiv domashn'ogo gospodarstva - moloka, ta produktiv pryrodnogo, lisovogo pohodzhennja – jagid i grybiv.

  2. Lectin histochemical studies on the vomeronasal organ of the sheep.

    PubMed

    Ibrahim, Dalia; Nakamuta, Nobuaki; Taniguchi, Kazumi; Taniguchi, Kazuyuki

    2013-01-01

    The vomeronasal organ of sheep was examined using lectin histochemistry in order to compare the types and amounts of the glycoconjugates among various components of the vomeronasal sensory and non-sensory epithelia. In the vomeronasal sensory epithelium, Dolichos biflorus agglutinin (DBA) stained particular cells, located at the same level as the vomeronasal receptor cells, while the distribution, shape and number of the stained cells did not correspond to those of the vomeronasal receptor cells. Datura stramonium lectin (DSL), Concanavalin A (Con A), Phaseolus vulgaris agglutinin-E (PHA-E) and Phaseolus vulgaris agglutinin-L (PHA-L) labeled the basal cells of both vomeronasal sensory and non-sensory epithelia. While, Wheat germ agglutinin (WGA), Succinylated-wheat germ agglutinin (s-WGA), Lycopersicon esculentum lectin (LEL), Solanum tuberosum lectin (STL) and Ricinus communis agglutinin-I (RCA-120) labeled the basal cells of the sensory epithelium, and Bandeiraea simplicifolia lectin-I (BSL-I) stained the basal cells of the non-sensory epithelium, respectively. Seventeen lectins labeled the free border of both vomeronasal sensory and non-sensory epithelia, while Sophora japonica agglutinin (SJA), Jacalin and Pisum sativum agglutinin (PSA) labeled neither free border of the sensory nor that of non-sensory epithelia. The expression pattern of glycoconjugate was similar, but not identical, in the free border between the sensory and non-sensory epithelia. These results indicate that there are dissimilar features in the type and amount of glycoconjugates between the vomeronasal sensory and non-sensory epithelia, and at the same time, among the various cell types either in the vomeronasal sensory or non-sensory epithelium.

  3. Heidal revisited: new light on critical elements in the allochthon of the classical Otta region (Oppland)

    NASA Astrophysics Data System (ADS)

    Heim, Michael; Corfu, Fernando

    2017-04-01

    lenses of serpentinite and augengneiss are also present. The unit possibly reflects high strain between middle and upper allochthon, resulting in repeated imbrication of different tectonic slices. Both Rudihø and Heidal units were, at a late stage of the medium-grade shear, intruded by numerous granitic to trondhjemitic dykes ranging from dm to hundreds of meters in size. Dating of these intrusives is made extremely difficult because of the pervasiveness of xenocrystic zircon, but the present available data suggest that they are Early Silurian (435-420 Ma), just like those in the Jotun nappe and the Trondheim region. Gjelsvik, T. 1947: Anorthosittkomplekset i Heidal. NGT 26. 58 pp. Siedlecka, A. et al. 1987: Berggrunnskart Lillehammer, 1:250 000. NGU. Strand, T. 1951: The Sel and Vågå map areas. Geology and petrology of a part of the Caledonides of Central Southern Norway. NGU 178, 117 pp.

  4. Impact of tsetse and trypanosomiasis control on cattle herd composition and calf growth and mortality at Arbaminch District (Southern Rift Valley, Ethiopia).

    PubMed

    Gechere, Geja; Terefe, Getachew; Belihu, Kelay

    2012-10-01

    The effect of tsetse/trypanosomiasis control on cattle herd composition and growth and mortality of calves in tsetse controlled (by Southern Tsetse Eradication Project (STEP)) and uncontrolled blocks in southern Ethiopia was assessed. Structured questionnaire was used to interview 182 households to estimate cattle herd composition and calf mortality. Calves were bled to examine the presence of trypanosomes by the buffy coat technique. Forty NGU traps were deployed and fly catches determined. A case-control study was performed on 40 calves for 6 months to estimate calve growth parameters. Accordingly, the mean cattle herd size was lower in tsetse-controlled block than in the uncontrolled block, whereas the relative number of calves in a herd tend to be higher in the tsetse-controlled block (P = 0.06). While there was no report of cattle mortality in tsetse-controlled block, 16.48 % of the respondents have lost calves in tsetse-uncontrolled block in 1 year time. The prevalence of trypanosome positive calves was 2.95 % for uncontrolled block but no positive case in tsetse-controlled block. The apparent densities of flies/trap/day in tsetse-uncontrolled block were 30-fold higher than in tsetse-controlled block (P < 0.01). The case-control study revealed that the mean body weight gain of calves in tsetse-controlled block (40.23 ± 0.7 kg) was significantly higher than that of the uncontrolled block (34.74 ± 0.68 kg). The above findings strongly suggest that the intervention by the STEP project has significantly reduced tsetse population and trypanosomiasis consequently contributing to improved calf growth and survival.

  5. Prevalence and correlates of Mycoplasma genitalium infection among prostatitis patients in Shanghai, China.

    PubMed

    Mo, Xiaohui; Zhu, Caixia; Gan, Jin; Wang, Chong; Wei, Fang; Gong, Weiming; Cai, Qiliang

    2016-07-04

    Background: Mycoplasma genitalium (M. genitalium) has been shown to be involved in chronic non-gonococcal urethritis (NGU). However, the prevalence and determinants of this emerging sexually transmissible infection among prostatitis patients remain obscure. Methods: Two hundred and thirty-five patients diagnosed with prostatitis and 152 health controls from sexually transmitted diseases (STD) clinics in Shanghai, China, were selected. M. genitalium was detected in the initial voided urine (VB1), midstream of urine (VB2), expressed prostatic secretion (EPS) and the opening urine after massage (VB3) by quantitative polymerase chain reaction (Q-PCR) targeting the Mycoplasma genitalium adhesion protein (MgPa). An infection of the prostate was considered positive if a uropathogen was found only in the EPS sample or VB3, or if it was at least four-fold greater in EPS or VB3 than in VB1 or VB2. The prostatitis patients with M. genitalium infection were treated with azithromycin. Results: The prevalence of M. genitalium infection was significantly higher among the prostatitis group than the control group (10 vs 3%, P = 0.005). Among the prostatitis group, M. genitalium infection was significantly associated with those patients who received treatment for genitourinary infection previously than those patients who did not (17 vs 6%; adjusted OR, 4.011; 95% CI, 1.562-10.300). The symptoms were totally or partially improved in 83% per cent (19/23) of prostatitis patients with M. genitalium, positive in EPS and M. genitalium turned negative after azithromycin treatment. Conclusions: M. genitalium was prevalent in the patients with prostatitis, particularly in those who received ineffective antibiotic treatment for the bacterium, and was identified as having a significant association of prostatitis.

  6. Monitoring and modelling snow avalanches in Svalbard

    NASA Astrophysics Data System (ADS)

    Humlum, O.; Christiansen, H.; Neumann, U.; Eckerstorfer, M.; Sjöblom, A.; Stalsberg, K.; Rubensdotter, L.

    2009-04-01

    Monitoring and modelling snow avalanches in Svalbard Ole Humlum 1,3, Hanne H. Christiansen 1, Ulrich Neumann 1, Markus Eckerstorfer 1, Anna Sjöblom 1, Knut Stalsberg 2 and Lena Rubensdotter 2. 1: The University Centre in Svalbard (UNIS). 2: Geological Survey of Norway (NGU) 3: University of Oslo Ground based transportation in Svalbard landscape all takes place across mountainous terrain affected by different geomorphological slope processes. Traffic in and around the Svalbard settlements is increasing, and at the same time global climate models project substantial increases in temperature and precipitation in northern high latitudes for coming century. Therefore improved knowledge on the effect of climatic changes on slope processes in such high arctic landscapes is becoming increasingly important. Motivated by this, the CRYOSLOPE Svalbard research project since 2007 has carried out field observations on snow avalanche frequency and associated meteorological conditions. Snow avalanches are important geomorphic agents of erosion and deposition, and have long been a source of natural disasters in many mid-latitude mountain areas. Avalanches as a natural hazard has thereby been familiar to inhabitants of the Alps and Scandinavia for centuries, while it is a more recent experience in high arctic Svalbard. In addition, overall climate, topography and especially high winter wind speeds makes it difficult to apply snow avalanche models (numerical or empirical) developed for use at lower latitudes, e.g. in central Europe. In the presentation we examplify results from the ongoing (since winter 2006-07) monitoring of snow avalanches in Svalbard along a 70 km long observational route in the mountains. In addition, we present observations on the geomorphological impact of avalanches, with special reference to the formation of rock glaciers. Finally, we also present some initial results from numerical attempts of snow avalanche risk modelling within the study area.

  7. European Plate Observing System - the Arctic dimension and the Nordic collaboration

    NASA Astrophysics Data System (ADS)

    Atakan, K.; Heikkinen, P.; Juhlin, C.; Thybo, H.; Vogfjord, K.

    2012-04-01

    strong motion networks monitor seismic activity and hazard in the North Atlantic. Vigorous volcanic activity along the plate boundary in Iceland and associated hazards are monitored by the Icelandic, seismic, geodetic, meteorological and hydrological networks. Recent eruptions, like the 2010 Eyjafjallajökull eruptions have demonstrated the far-reaching hazard to aviation caused by volcanic eruptions in Iceland. The high-sensitivity seismic and geodetic networks of Sweden monitor isostatic rebound of Fennoscandia. In this context, the varied Nordic monitoring networks provide a significant contribution to the main objectives of EPOS. There are already existing links with the other ESFRI initiatives where strong Nordic participation is established, such as SIOS and EMSO. As such EPOS provides the necessary platform to collaborate and develop an important Nordic dimension in the European Research Area. There is a long tradition of collaboration at the governmental level between the Nordic countries, Norway, Sweden, Denmark, Finland and Iceland. Within the fields of research and education, the Nordic Ministries have a dedicated program, where research networks are being promoted. Recently a Nordic collaborative network in seismology, "NordQuake" (coordinated by Denmark) was established within this program. This collaboration which is now formalized and supported by the Nordic Ministries is based on a cooperation which was initiated more than 40 years ago, where annual Nordic Seminars in seismology (previously on detection seismology) was the central element. EPOS Nordic collaboration, building upon a long lasting history, has a significant potential for synergy effects in the region and therefore represents an important dimension within EPOS. Nordic EPOS Team: Lars Ottemöller (UiB), Mathilde B. Sørensen (UiB), Louise W. Bjerrum (UiB), Conrad Lindholm (Norsar), Halfdan Kjerulf (SK), Amir Kaynia (NGI), Valerie Maupin (UiO), Tor Langeland (CMR), Joerg Ebbing (NGU), John

  8. Identification and characterization of microRNAs in the pancreatic fluke Eurytrema pancreaticum

    PubMed Central

    2013-01-01

    Background Eurytrema pancreaticum is one of the most common flukes, which mainly infects ruminants globally and infects human beings accidentally; causing eurytremiasis that has high veterinary and economic importance. MicroRNAs (miRNAs) are small non-coding RNAs and are now considered as a key mechanism of gene regulation at the post-transcription level. Methods We investigated the global miRNA expression profile of E. pancreaticum adults using next-generation sequencing technology combined with real-time quantitative PCR. Results By using the genome of the closely-related species Schistosoma japonicum as reference, we obtained 27 miRNA candidates out of 16.45 million raw sequencing reads, with 13 of them found as known miRNAs in S. japonicum and/or S. mansoni, and the remaining 14 miRNAs were considered as novel. Five out of the 13 known miRNAs coming from one family named as sja-miR-2, including family members from miR-2a to miR-2e. Targets of 19 miRNAs were successfully predicated out of the 17401 mRNA and EST non-redundant sequences of S. japonicum. It was found that a significant high number of targets were related to “chch domain-containing protein mitochondrial precursor” (n = 29), “small subunit ribosomal protein s30e” (n = 21), and “insulin-induced gene 1 protein” (n = 9). Besides, “egg protein cp3842” (n = 2), “fumarate hydratase” (n = 2), “ubiquitin-conjugating enzyme” (n = 2), and “sperm-associated antigen 6” (n = 1) were also found as targets of the miRNAs of E. pancreaticum. Conclusions The present study represents the first global characterization of E. pancreaticum miRNAs, which provides novel resources for a better understanding of the parasite, which, in turn, has implications for the effective control of the disease it causes. PMID:23351883

  9. IL-24 Inhibits Lung Cancer Cell Migration and Invasion by Disrupting The SDF-1/CXCR4 Signaling Axis

    PubMed Central

    Panneerselvam, Janani; Jin, Jiankang; Shanker, Manish; Lauderdale, Jason; Bates, Jonathan; Wang, Qi; Zhao, Yan D.; Archibald, Stephen J.; Hubin, Timothy J.; Ramesh, Rajagopal

    2015-01-01

    Background The stromal cell derived factor (SDF)-1/chemokine receptor (CXCR)-4 signaling pathway plays a key role in lung cancer metastasis and is molecular target for therapy. In the present study we investigated whether interleukin (IL)-24 can inhibit the SDF-1/CXCR4 axis and suppress lung cancer cell migration and invasion in vitro. Further, the efficacy of IL-24 in combination with CXCR4 antagonists was investigated. Methods Human H1299, A549, H460 and HCC827 lung cancer cell lines were used in the present study. The H1299 lung cancer cell line was stably transfected with doxycycline-inducible plasmid expression vector carrying the human IL-24 cDNA and used in the present study to determine the inhibitory effects of IL-24 on SDF-1/CXCR4 axis. H1299 and A549 cell lines were used in transient transfection studies. The inhibitory effects of IL-24 on SDF1/CXCR4 and its downstream targets were analyzed by quantitative RT-PCR, western blot, luciferase reporter assay, flow cytometry and immunocytochemistry. Functional studies included cell migration and invasion assays. Principal Findings Endogenous CXCR4 protein expression levels varied among the four human lung cancer cell lines. Doxycycline-induced IL-24 expression in the H1299-IL24 cell line resulted in reduced CXCR4 mRNA and protein expression. IL-24 post-transcriptionally regulated CXCR4 mRNA expression by decreasing the half-life of CXCR4 mRNA (>40%). Functional studies showed IL-24 inhibited tumor cell migration and invasion concomitant with reduction in CXCR4 and its downstream targets (pAKTS473, pmTORS2448, pPRAS40T246 and HIF-1α). Additionally, IL-24 inhibited tumor cell migration both in the presence and absence of the CXCR4 agonist, SDF-1. Finally, IL-24 when combined with CXCR4 inhibitors (AMD3100, SJA5) or with CXCR4 siRNA demonstrated enhanced inhibitory activity on tumor cell migration. Conclusions IL-24 disrupts the SDF-1/CXCR4 signaling pathway and inhibits lung tumor cell migration and invasion

  10. The Fe Group Abundances in the B3 IV Standard ι Herculis Determined from ASTRAL II Observations

    NASA Astrophysics Data System (ADS)

    Peters, Geraldine J.; Proffitt, Charles R.; Adelman, Saul J.; Ayres, Thomas R.

    2017-01-01

    Iota Herculis is an ultrasharp-lined B3 IV star that historically has been considered as an abundance standard for the early B stars. This star was one of the targets in the HST Treasury Program Advanced Spectral Library II: Hot Stars (ASTRAL II) that produced uninterrupted spectra of high to medium resolution in the region 1150-3100 Å. The abundances for the Fe group elements (Ti, V, Cr, Mn, Fe, Co, & Ni) in ι Her were determined mostly from STIS E140H and E230H (resolving power of 114,000) observations. Measurable lines from the Fe group, except for a very few multiplets of Fe II, III are not found in optical spectra. Whereas the light elements are delivered to the ISM by core-collapse supernovae (CCSNe), the Fe group elements are believed to come mostly from low/intermediate mass binaries containing white dwarfs that undergo SNe Ia explosions. A single SNe Ia can deliver 0.5 solar masses of pure Fe (and maybe Mn) to the ISM compared with about 0.07 solar masses from a CCSNe. The HST/STIS data were supplemented with optical spectra obtained at the Dominion Astrophysical Observatory (resolving power about 60,000). The abundance analysis was carried through with the NLTE code TLUSTY/SYNSPEC (Hubeny & Lanz, ApJ, 439,875,1995). The model parameters adopted for the ι Her are Teff = 17750 ± 250 K, log g = 3.75 ± 0.05 dex, Vturb = 0 km s-1, and v sin i = 5 km s-1. Solar abundances appear to prevail for the lighter elements but the abundances of Fe group elements are 0.3 - 0.7 dex below solar values determined by Grevesse et al. (2010, Ap&SpSci, 328, 179). It appears that ι Her was formed in a region our Galaxy mostly enriched by CCSNe.The authors appreciate support from STScI grants HST-GO-09848 and HST-GO-13346. SJA was a guest observer at DAO.

  11. International Centre for Geohazards (ICG) Established at the Norwegian Geotechnical Institute in Oslo, Norway.

    NASA Astrophysics Data System (ADS)

    Solheim, A.; Nadim, F.

    2003-12-01

    As one of 13 new `Centres of Excellence' awarded by the Norwegian Research Council with a 10-year funding schedule, the International Centre for Geohazards (ICG) was established at the Norwegian Geotechnical Institute (NGI), in January 2003. The Centre is formed through a co-operation between several institutions, which in addition to NGI are the Gelogical Survey of Norway (NGU), Norwegian Seismic Array (NORSAR), University of Oslo (UiO) and the Norwegian University for Science and Technology (NTNU). The Centre is located in the NGI building in Oslo, Norway. Funding is for 10 years, and the centre is staffed by researchers from the partner institutions, visiting scientists, post-doctoral fellows and Ph.D. students. With the ultimate goal of geohazard mitigation and preventing the loss of lives and damage to infrastructure and environment, key research topics of the Centre are: Unsaturated soils and mechanisms for precipitation-induced slides in steep slopes; Risk and vulnerability analysis for geohazards; Earthquake hazard, vulnerability and risk evaluation; Rock slope failures - models and risks; Landslides in soft clay slopes (quick clay), fjord margins and coastal zones; GIS applications to geohazards; SAR applications to geohazards; Slide dynamics and mechanics of disintegration; Tsunami modelling and prediction; and Offshore Geohazards. As prospecting for hydrocarbons move into increasingly deeper waters of the world's continental margins, research on offshore geohazard forms an important activity of the new centre. Main offshore geohazards include slope instability, effects of shallow gas and gas hydrates on the behaviour of seafloor sediments, mud volcanism and diapirism. Of these, slope instability is considered to be the major hazard, because of the potentially serious third party impact. The current offshore geohazards project within ICG consists of three main themes: Assessment of offshore geohazards (site surveys); Geophysical methods for offshore

  12. Bovine trypanosomosis and vector density in Omo-Ghibe tsetse belt, South Ethiopia.

    PubMed

    Abebe, Rahmeto; Gute, Solomon; Simon, Ijigu

    2017-03-01

    African animal trypanosomosis (AAT) is a parasitic disease that causes serious economic losses in livestock from anemia, loss of condition, emaciation and death in untreated cases. It is one of the major constraints to improved livestock production and productivity in Ethiopia. Entomological and parasitological surveys were conducted in the Omo-Ghibe tsetse belt of south Ethiopia to estimate the prevalence of bovine trypanosomosis and the apparent tsetse density (AD), and identify the potential risk factors. For the parasitological study, blood samples were collected from 1508 cattle sampled from 11 districts and assayed using the buffy coat technique and Giemsa-stained thin smears. For the entomological survey, a total of 216 biconical and NGU traps were deployed in all districts. The overall animal-level prevalence of trypanosomosis was 7.8% (95% CI: 6.5, 9.3). The trypanosome species identified were Trypanosoma congolense (75.4%), T. vivax (20.3%), T. brucei brucei (1.7%) and mixed T. congolense/T. vivax (2.6%). Regarding the entomological survey result, a total of 2243 tsetse flies were captured which identified to be Glossina pallidipes (85.1%) and G. f. fuscipes (14.9%). Besides, other biting flies of the genus Stomoxys (n=146) and Tabanus (n=17) were also trapped. The AD noted in the present study was 3.5 flies/trap/day. Both the prevalence of trypanosomosis and AD of tsetse flies were significantly (p<0.05) influenced by altitude. The prevalence of trypanosomosis was also significantly (p<0.05) associated with poor body condition score, black coat color and lower mean packed cell volume while no significant prevalence difference was noted along with age and sex category. In conclusion, the present study suggested that trypanosomosis is an important disease of cattle in the Omo-Ghibe tsetse belt in dry season. The disease is mainly caused by the most pathogenic T. congolense and transmission is predominantly by tsetse flies, particularly G. pallidipes. The

  13. Geo-Seas - building a unified e-infrastructure for marine geoscientific data management in Europe

    NASA Astrophysics Data System (ADS)

    Glaves, H.; Schaap, D.

    2012-04-01

    collaborative links with other European projects including EMODNET, Eurofleets. Genesi-DEC and iMarine as well as extending to the wider marine geoscientific and oceanographic community including projects in the USA such as the Rolling Deck Repository (R2R) initiative and also organisations in both the USA and Australia. On behalf of the Geo-Seas consortium partners: NERC-BGS (United Kingdom), NERC-BODC (United Kingdom), NERC-NOCS (United Kingdom), MARIS (Netherlands), IFREMER (France), BRGM (France), TNO (Netherlands), BSH (Germany), IGME (Spain), LNEG (Portugal), GSI (Ireland), BGR (Germany), OGS (Italy), GEUS (Denmark), NGU (Norway), PGI (Poland), EGK (Estonia), NRC-IGG (Lithuania), IO-BAS (Bulgaria), NOA (Greece), CIRIA (United Kingdom), MUMM (Belgium), UB (Spain), UCC (Ireland), EU-Consult (Netherlands), CNRS (France), SHOM (France), CEFAS (United Kingdom), and LU (Latvia).

  14. P-Cable: New High-Resolution 3D Seismic Acquisition Technology

    NASA Astrophysics Data System (ADS)

    Planke, Sverre; Berndt, Christian; Mienert, Jürgen; Bünz, Stefan; Eriksen, Frode N.; Eriksen, Ola K.

    2010-05-01

    ø, VBPR, P-Cable 3D Seismic AS (P3S), and IFM-GEOMAR. Presently, a Norwegian national infrastructure consortium (Univ. of Tromsø, P3S, Univ. of Bergen, NGU) assembles a mobile P-Cable2 high-resolution 3D seismic system for fully operational use of the technology for scientific purposes.

  15. The hazard and risk classification for large unstable rock slopes in Norway

    NASA Astrophysics Data System (ADS)

    Hermanns, R. L.; Oppikofer, T.; Anda, E.; Berg, H.; Blikra, L. H.; Böhme, M.; Bunkholt, H.; Crosta, G. B.; Dahle, H.; Fischer, L.; Jaboyedoff, M.; Loew, S.; Yugsi Molina, F. X.

    2012-12-01

    Catastrophic failure of large rock slopes led several times per century in Norway to rock avalanches or large rock falls, which impacted into settlements directly but also caused either a displacement wave when impacting a water body or damming of narrow valleys that impacred settlements in distance with often deadly consequences. Such events will also occur in future. In order to make mitigation of the negative consequences of such events possible, the Geological Survey of Norway (NGU) following its obligation towards society and the Norwegian Water and Energy Directorate (NVE) carries out systematic geologic mapping of potentially unstable rock slopes that might fail catastrophically. Within the last years systematic mapping in only three of the 17 counties of Norway has revealed more than 300 sites of potential future rock slope failures. This number necessitates prioritization of follow-up activities, such as periodic and permanent monitoring and early-warning. This quantitative hazard and risk classification system was build to compare sites so that political and financial decisions can be taken. The classification system is scenario-based as intensity and rate of displacement, as well as the geological structures activated by the sliding rock mass vary significantly on slopes. In addition, for each scenario the secondary effects, such as generation of displacement waves or landslide damming of valleys with the potential of later outburst floods, have to be evaluated. The hazard classification is based on two sets of criteria: 1) Structural site investigations including analysis of the development of the back-scarp, lateral limits and basal sliding surface. This includes a kinematic analysis that tests if rock sliding or toppling is kinematically feasible with respect to the slope orientation, the persistence of main structures and the morphologic expression of the sliding surface. 2) The analysis of the activity of the slope is primarily based on the slide

  16. Studying the basement structure of the Ramså field on Andøya, northern Norway

    NASA Astrophysics Data System (ADS)

    Broenner, M.; Olesen, O.; Koziel, J.; Gellein, J.; Midbøe, P.; Henningsen, T.

    2009-12-01

    Whereas offshore on the Norwegian shelf and between the islands and the mainland deep Mesozoic basins still exist, Mesozoic sediments are generally absent on mainland Norway due to erosion processes. The Ramså field on Andøya, Vesterålen, Northern Norway is the only place in Norway with exposed Mesozoic rocks on land and thus provides an excellent place to study extensively the link between basement structures and sedimentary successions. A combined observation and interpretation of faulting and basement surfaces as well as a better knowledge of the different basement types allow a more accurate and differentiated interpretation of the tectonic setting and evolution of the region. With the aim to gain a better knowledge of the basement and the sedimentary structures, new high resolution aeromagnetic data and densely measured gravity data were acquired on Andøya, processed and interpreted to update the present geotectonic picture for the Ramså field. Magnetic data were used to map the structural elements of the area and to link them with geological observations. Furthermore Euler deconvolution techniques were applied to estimate the depth to magnetic sources and to identify fault systems. Gravity data were most sensitive to the varying sediment thickness and were consequently helpful to map the depth-to-basement. Stratigraphic information from four wells, drilled down to and into different basement types were used to establish a structural layer model of the Ramså field, which were assigned with petrophysical parameters based on NGU's comprehensive petrophysical database. The Ramså field is generally considered to represent a down-faulted block, bounded by sharp and steep faults to the surrounding exposed basement blocks. But the structural models, so far based on surface observations and well correlations, were never controlled and refined by using gravity and magnetic data. State of the art techniques and densely measured potential field data have the

  17. Robust control methods for nonlinear systems with uncertain dynamics and unknown control direction

    NASA Astrophysics Data System (ADS)

    Ton, Chau T.

    consideration is required in control design for systems that also include unknown bounded disturbances. To cope with these challenges, a robust continuous controller is developed using an ISMC technique, which achieves asymptotic trajectory tracking for systems with unknown bounded disturbances, while simultaneously compensating for parametric uncertainty in the input gain matrix. The ISMC design is rigorously proven to achieve asymptotic trajectory tracking for a quadrotor system and a synthetic jet actuator (SJA)-based aircraft system. In the ISMC designs, it is assumed that the signs in the uncertain input-multiplicative gain matrix (i.e., the actuator control directions) are known. A much more challenging scenario is encountered in designing controllers for classes of systems, where the uncertainty in the input gain matrix is extreme enough to result in an a priori-unknown control direction. Such a scenario can result when dealing with highly inaccurate dynamic models, unmodeled parameter variations, actuator anomalies, unknown external or internal disturbances, and/or other adversarial operating conditions. To address this challenge, a SMCbased self-recongurable control algorithm is presented, which automatically adjusts for unknown control direction via periodic switching between sliding manifolds that ultimately forces the state to a converging manifold. Rigorous mathematical analyses are presented to prove the theoretical results, and simulation results are provided to demonstrate the effectiveness of the three proposed control algorithms.

  18. Trans people's experiences with assisted reproduction services: a qualitative study.

    PubMed

    James-Abra, S; Tarasoff, L A; Green, D; Epstein, R; Anderson, S; Marvel, S; Steele, L S; Ross, L E

    2015-06-01

    assisting trans people to become parents through the use of AR, our study demonstrates that they are already accessing or attempting to access these services. This reality necessitates a shift toward exploring the ways in which AR services can be improved to better meet the needs of this population, from the perspectives of both service users and service providers. This project was supported by the Canadian Institutes of Health Research-Institute of Gender and Health, in partnership with the Assisted Human Reproduction Canada: Catalyst Grant: Psychosocial Issues Associated with Assisted Human Reproduction (FRN-103595). S.M. was supported by a Canada Graduate Scholarship from the Social Science and Humanities Research Council, as well as research funding from Osgoode Hall Law School, York University. S.J.-A. was supported by an Ontario Graduate Scholarship funded by the Province of Ontario and the University of Toronto. N/A. © The Author 2015. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  19. Web-GIS approach for integrated analysis of heterogeneous georeferenced data

    NASA Astrophysics Data System (ADS)

    Okladnikov, Igor; Gordov, Evgeny; Titov, Alexander; Shulgina, Tamara

    2014-05-01

    atmospheric composition and climate (MACC) Collaborated Project, NOAA-CIRES Twentieth Century Global Reanalysis Version II, NCEP Climate Forecast System Reanalysis (CFSR), meteorological observational data for the territory of the former USSR for the 20th century, results of modeling by global and regional climatological models, and others are available for processing by the system. The Web-GIS information-computational system for heterogeneous geophysical data analysis provides specialists involved into multidisciplinary research projects with reliable and practical instruments for integrated research of climate and ecosystems changes on global and regional scales. With its help even an unskilled in programming user is able to process and visualize multidimensional observational and model data through unified web-interface using a common graphical web-browser. This work is partially supported by SB RAS project VIII.80.2.1, RFBR grant #13-05-12034, grant #14-05-00502, and integrated project SB RAS #131. References 1. Gordov E.P., Lykosov V.N., Krupchatnikov V.N., Okladnikov I.G., Titov A.G., Shulgina T.M. Computational and information technologies for monitoring and modeling of climate changes and their consequences. - Novosibirsk: Nauka, Siberian branch, 2013. - 195 p. (in Russian) 2. Felice Frankel, Rosalind Reid. Big data: Distilling meaning from data // Nature. Vol. 455. N. 7209. P. 30. 3. T.M. Shulgina, E.P. Gordov, I.G. Okladnikov, A.G., Titov, E.Yu. Genina, N.P. Gorbatenko, I.V. Kuzhevskaya, A.S. Akhmetshina. Software complex for a regional climate change analysis. // Vestnik NGU. Series: Information technologies. 2013. Vol. 11. Issue 1. P. 124-131 (in Russian). 4. I.G. Okladnikov, A.G. Titov, T.M. Shulgina, E.P. Gordov, V.Yu. Bogomolov, Yu.V. Martynova, S.P. Suschenko, A.V. Skvortsov. Software for analysis and visualization of climate change monitoring and forecasting data // Numerical methods and programming, 2013. Vol. 14. P. 123-131 (in Russian).

  20. Variations in Pb concentrations and Pb-isotope ratios in soils collected along an east-west transect across the United States

    NASA Astrophysics Data System (ADS)

    Smith, David; Woodruff, Laurel; Reimann, Clemens; Flem, Belinda

    2014-05-01

    Soil A-horizon and C-horizon samples were collected along a 4000 km long transect cutting the USA from the west to the east coast. For purposes of site selection, the transect was divided into approximately 40-km segments. For each segment, a 1-km2 target area was selected at random. Soil A- and C-horizon samples were collected at a site within each target area that was most representative of the surrounding landscape. The samples were air-dried at ambient temperature, disaggregated, and sieved through a 2-mm stainless steel screen. The <2-mm material was crushed to <150 µm in a ceramic mill prior to chemical analysis. Lead was analyzed in all the A- and C-horizon samples by inductively coupled plasma-mass spectrometry following a 4-acid digestion. The complete dataset can be found in Smith et al., 2005. Pb-isotope ratio measurements were carried out on 159 soil A-horizon and 137 soil C-horizon samples on an inductively coupled sector field plasma mass spectrometer (SF-ICP-MS; ELEMENT 1, Finnigan MAT) in the laboratory of the Geological Survey of Norway (NGU), following a 7 N HNO3 digestion. Lead concentrations along the transect show (1) generally higher values in the soil A-horizon than the C-horizon (median 21 vs. 16.5 mg/kg), (2) an increase in the median value of the soil A-horizon for the central to eastern U.S. (Missouri to Maryland) when compared to the western U.S. (California to Kansas) (median 26 vs. 20 mg/kg) and (3) a higher A/C ratio for the central to eastern US (1.35 vs. 1.14). Lead isotopes show a distinct trend across the U.S., with the highest 206Pb/207Pb ratios occurring in the centre (Missouri, median A-horizon: 1.245; C-horizon: 1.251) and the lowest at both coasts (e.g. California, median A-horizon: 1.195; C-horizon:1.216). The soil C-horizon samples show generally higher 206Pb/207Pb ratios than the A-horizon (median C-horizon: 1.224; A-horizon: 1.219). The 206Pb/207Pb isotope ratios in the soil A horizon show a correlation with the total

  1. Silurian and Devonian in Vietnam—Stratigraphy and facies

    NASA Astrophysics Data System (ADS)

    Thanh, Tống Duy; Phương, Tạ Hoàng; Janvier, Philippe; Hùng, Nguyễn Hữu; Cúc, Nguyễn Thị Thu; Dương, Nguyễn Thùy

    2013-09-01

    Silurian and Devonian deposits in Viet Nam are present in several zones and regions, including Quang Ninh, East Bac Bo, and West Bac Bo Zones of the Bac Bo Region, the Dien Bien-Nghe An and Binh Tri Thien Zones of the Viet-Lao Region, and the South Trung Bo, and Western Nam Bo Zones of the South Viet Nam Region (Fig. 1). The main lithological features and faunal composition of the Silurian and Devonian Units in all these zones are briefly described. The Silurian consists of deep-water deposits of the upper parts of the Co To and Tan Mai Formations in the Quang Ninh Zone, the upper parts of the Phu Ngu Formation in the East Bac Bo Zone and the upper parts of the Long Dai and Song Ca Formations in the Viet-Lao Region. Shallow water facies Silurian units containing benthic faunas are more widely distributed, including the upper part of the Sinh Vinh and Bo Hieng Formations in the West Bac Bo Zone, the Kien An Formation in the Quang Ninh Zone, and, in the Viet-Lao Region, the Dai Giang Formation and the upper part of the Tay Trang Formation. No Lower and Middle Devonian deposits indicate deep water facies, but they are characterized by different shallow water facies. Continental to near shore, deltaic facies characterize the Lower Devonian Song Cau Group in the East Bac Bo Zone, the Van Canh Formation in the Quang Ninh Zone, and the A Choc Formation in the Binh Tri Thien Zone. Similar facies also occur in the Givetian Do Son Formation of the Quang Ninh Zone, and the Tan Lap Formation in the East Bac Bo Zone, and consist of coarse terrigenous deposits—cross-bedded conglomerates, sandstone, etc. Most Devonian units are characterized by shallow marine shelf facies. Carbonate and terrigenous-carbonate facies dominate, and terrigenous facies occur in the Lower and Middle Devonian sections in some areas only. The deep-water-like facies is characteriztic for some Upper Devonian formations in the Bac Bo (Bang Ca and Toc Tat Formations) and Viet-Lao Regions (Thien Nhan and

  2. Hyperextension in the pre-Caledonian margin of Baltica: new observations and U-Pb ages from the Samnanger Complex, Major Bergen Arc, western Norway.

    NASA Astrophysics Data System (ADS)

    Jakob, Johannes; Alsaif, Manar; Andersen, Torgeir; Corfu, Fernando

    2015-04-01

    -term weakening of the crust and zones of exhumed mantle may therefore be prone to localized compressive deformation during later stages of a Wilson Cycle. The Ordovician magmatic rocks within the SC might therefore be produced by incipient subduction, which was contemporaneous with the formation of supra-subduction ophiolite and island arc complexes in the oceanic terranes (e.g. Slagstad et al., 2014). Andersen, T.B. et al., 2012. Evidence for hyperextension along the pre-Caledonian margin of Baltica. Journal of the Geological Society, 169, pp.601-612. Færseth, R.B. et al., 1977. Geology of the lower paleozoic rocks in the Samnanger-Osterøy Area, Major Bergen Arc, Western Norway. Offprint NGU, 334, pp.19-58. Slagstad, T. et al., 2015. Tectonomagmatic evolution of the Early Ordovician suprasubduction-zone ophiolites of the Trondheim Region, Mid-Norwegian Caledonides. In: F. Corfu, D. Gasser, & D. M. Chew, eds. New Perspectives on the Caledonides of Scandinavia and Related Areas. Geological Society, London, Speacial Publications 390, pp. 541-561.

  3. Ethical questions in landslide management and risk reduction in Norway

    NASA Astrophysics Data System (ADS)

    Taurisano, A.; Lyche, E.; Thakur, V.; Wiig, T.; Øvrelid, K.; Devoli, G.

    2012-04-01

    The loss of lives caused by landslides in Norway is smaller than in other countries due to the low population density in exposed areas. However, annual economic losses from damage to properties and infrastructures are vast. Yet nationally coordinated efforts to manage and reduce landslide and snow avalanche risk are a recent challenge, having started only in the last decade. Since 2009, this has been a task of the Norwegian Water Resources and Energy Directorate (NVE) under the Ministry of Petroleum and Energy. Ongoing work includes collection of landslide data, production of susceptibility and hazard maps, planning of mitigation measures along with monitoring and early warning systems, assistance to areal planning, providing expertise in emergencies and disseminating information to the public. These activities are realized in collaboration with the Norwegian Geological Survey (NGU), the Meteorological Institute, the Road and Railway authorities, universities and private consultant companies. As the total need for risk mitigating initiatives is by far larger than the annual budget, priority assessment is crucial. This brings about a number of ethical questions. 1. Susceptibility maps have been produced for the whole country and provide a first indication of areas with potential landslide or snow avalanche hazard, i.e. areas where special attention and expert assessments are needed before development. Areas where no potential hazard is shown can in practice be developed without further studies, which call for relatively conservative susceptibility maps. However, conservative maps are problematic as they too often increase both cost and duration of building projects beyond the reasonable. 2. Areas where hazard maps or risk mitigation initiatives will be funded are chosen by means of cost-benefits analyses which are often uncertain. How to estimate the benefits if the real probability for damage can only be judged on a very subjective level but not really calculated

  4. Holocene to contemporary source-to-sink fluxes in a valley-fjord system in western Norway: Erdalen and Bødalen site project (SedyMONT - IP Norway)

    NASA Astrophysics Data System (ADS)

    Beylich, A. A.; Laute, K.; Liermann, S.

    2012-04-01

    The focus of this Norwegian Research Council (NFR) funded Norwegian Individual Project (IP) (http://www.ngu.no/sedymont) within the European Science Foundation (ESF) SedyMONT (http://www.sedymont.eu) (EUROCORES TOPO-EUROPE) Programme (http://www.esf.org/topoeurope) is on Holocene, subrecent and contemporary sedimentary fluxes and budgets in the Erdalen and Bødalen valley systems in Nordfjord, western Norway. The innovative approach of this international research project is the integrated quantitative study of longer-term (Holocene), subrecent and contemporary source-to-sink fluxes and geomorphic process rates in selected representative valley-fjord systems using a novel combination of advanced methods and techniques. With respect to the main aims and objectives of ESF SedyMONT, the following main aims of the Erdalen and Bødalen site project can be stressed: - Analyse how the inheritance of the landscape due to the influence of the Last Glacial Maximum (LGM) has affected process rates over time (paraglacial system), - Document changes in process rates over different timescales by combining quantitative knowledge on Holocene process rates with newly generated data on subrecent and contemporary process rates. High-resolution monitoring of denudative surface processes in Erdalen and Bødalen, in combination with repeated analyses of surface water chemistry, atmospheric solute inputs and granulometric as well as shape analyses of suspended sediments provide high-resolution data to analyse and quantify present-day sedimentary and solute fluxes as well as sediment sources, denudation rates, and meteorological and topographical / landscape morphometric controls of denudative processes. In addition to standard methods for monitoring bedload transport, innovative techniques like horizontally installed impact sensors and biofilm analysis are applied in combination with advanced flume experiments (for calibration of field data) to analyse channel stability / mobility and to

  5. Glacial isostatic adjustment and Holocene to contemporary source-to-sink fluxes in valley-fjord systems in western Norway

    NASA Astrophysics Data System (ADS)

    Beylich, Achim A.; Laute, Katja; Liermann, Susan

    2013-04-01

    The focus of this Norwegian Research Council (NFR) funded Norwegian Individual Project (http://www.ngu.no/sedymont) within the European Science Foundation (ESF) SedyMONT (as part of EUROCORES TOPO-EUROPE) Programme (http://www.esf.org/topoeurope) is on Holocene, subrecent and contemporary sedimentary fluxes and budgets in selected valley-fjord systems (Erdalen and Bødalen) in Nordfjord, western Norway. The following main aims can be pointed out: - Analyse in which way glacial isostatic adjustment and surface denudational processes have been interacting over the Holocene - Analyse how the inheritance of the landscape due to the influence of the Last Glacial Maximum (LGM) has affected surface process rates over time - Document changes in surface process rates over different timescales by combining knowledge on Holocene surface process rates with data on subrecent and contemporary surface process rates High-resolution monitoring of denudational surface processes in the Erdalen and Bødalen drainage basin systems, in combination with repeated analyses of surface water chemistry, atmospheric solute inputs and granulometric as well as shape analyses of suspended sediments provide high-resolution data to analyse and quantify present-day sedimentary and solute fluxes as well as sediment sources, denudation rates, and meteorological and topographical / landscape morphometric controls of denudational surface processes. In addition to standard methods for monitoring bedload transport, innovative techniques like impact sensors and biofilm analysis are applied in combination with advanced flume experiments (for calibration of field data) to analyse channel stability / mobility and to estimate bedload transport rates in both drainage basin systems. Lakes are functioning as significant sediment traps within both drainage basins and the volume and composition of lake sediments are studied using echo-sounder, georadar and different coring techniques. Investigations on volumes and

  6. Circum-Arctic mapping project: new magnetic anomaly map of the Arctic (to 60 degrees N)

    NASA Astrophysics Data System (ADS)

    Gaina, Carmen

    2010-05-01

    An international effort to compile Circum-Arctic geophysical and bedrock data has been conducted by several national agencies (Russia-VSEGEI and VNIIO, Sweden-SGU, Finland-GTK, Denmark-GEUS, USA-USGS, Canada-GSC, Germany-BGR and Norway-NGU) since 2005. This project aims to produce an atlas that will comprise geological and geophysical digital maps at a scale of 1: 5 million scale for the Arctic region limited by the 60 degree North latitude. New published and classified magnetic anomaly gridded data from each participant group were gathered and converted to a common datum (WGS84) and format. The Greenland region magnetic anomaly grid (Verhoef et al., 1996) has been updated with new aeromagnetic surveys performed in West Greenland between 1992-2001 (Rasmussen, 2002), and in the Nares Strait area (Damaske & Oakey, 2006; Oakey & Damaske, 2006). The oceanic area east of Greenland (NE Atlantic) contains most of the aeromagnetic data used in the Verhoef et al., (1996)'s compilation (pre-1990) plus new aeromagnetic surveys over offshore Norway collected up to 2007 (Olesen et al., 1997; Olesen et al., 2007; Gernigon et al., 2008). The gridded data has been upward continued to 1 km above ground or sea-level and trimmed around the areas of major overlaps. The Alaska USGS aeromagnetic compilation has been used as the "master grid" for merging the major gridded data sets together and the downward continued lithospheric magnetic field model MF6 derived from satellite data (Maus et al., 2008) has been used as a regional reference surface. We have used a blending function over the area of overlap in order to smooth the transition from one grid to the other (GridKnit, GEOSOFT). The resulting grid has been re-sampled to a 2 km grid cell. In order to construct the final Circum-Arctic magnetic anomaly grid (CAMP-M) we have adopted the approach used by several research groups for compiling the World Digital Magnetic Anomaly Map (WDMAM) and used near-surface magnetic data for the short

  7. Geo-Seas - a pan-European infrastructure for the management of marine geological and geophysical data.

    NASA Astrophysics Data System (ADS)

    Glaves, Helen; Graham, Colin

    2010-05-01

    countries. This makes the direct use of primary data in an integrated way very difficult and also hampers use of the data sets in a harmonised way to produce multidisciplinary data products and services. To ensure interoperability with other marine environmental data types Geo-Seas ISO19115 metadata, OGC and GeoSciML standards will be used as the basis for the metadata profiles for the geological and geophysical data. This will be largely achieved by modifying the SeaDataNet metadata standard profile (Common Data Index or CDI), which is itself based upon the ISO19115 standard, to accommodate the requirements of the Geo-Seas project. The overall objective of Geo-Seas project is to build and deploy a unified marine geoscientific data infrastructure within Europe which will in effect provide a data grid for the sharing of marine geological and geophysical data. This will result in a major improvement in the locating, accessing and delivery of federated marine geological and geophysical data and data products from national geological surveys and research institutes across Europe. There is an emphasis on interoperability both with other disciplines as well as with other key framework projects including the European Marine Observation and Data Network (EMODNet) and One Geology - Europe. In addition, a key objective of the Geo-Seas project is to underpin European directives such as INSPIRE as well as recent framework programmes on both the global and European scale, for example Global Earth Observation System of Systems (GEOSS) and Global Monitoring for Environment and Security (GMES), all of which are intended to encourage the exchange of data and information. Geo-Seas consortium partners: NERC-BGS (United Kingdom), NERC-BODC (United Kingdom), NERC-NOCS (United Kingdom), MARIS (Netherlands), IFREMER (France), BRGM (France), TNO (Netherlands), BSH (Germany), IGME (Spain), INETI (Portugal), IGME (Greece), GSI (Ireland), BGR (Germany), OGS (Italy), GEUS (Denmark), NGU (Norway), PGI

  8. Consequence assessment of large rock slope failures in Norway

    NASA Astrophysics Data System (ADS)

    Oppikofer, Thierry; Hermanns, Reginald L.; Horton, Pascal; Sandøy, Gro; Roberts, Nicholas J.; Jaboyedoff, Michel; Böhme, Martina; Yugsi Molina, Freddy X.

    2014-05-01

    Steep glacially carved valleys and fjords in Norway are prone to many landslide types, including large rockslides, rockfalls, and debris flows. Large rockslides and their secondary effects (rockslide-triggered displacement waves, inundation behind landslide dams and outburst floods from failure of landslide dams) pose a significant hazard to the population living in the valleys and along the fjords shoreline. The Geological Survey of Norway performs systematic mapping of unstable rock slopes in Norway and has detected more than 230 unstable slopes with significant postglacial deformation. This large number necessitates prioritisation of follow-up activities, such as more detailed investigations, periodic displacement measurements, continuous monitoring and early-warning systems. Prioritisation is achieved through a hazard and risk classification system, which has been developed by a panel of international and Norwegian experts (www.ngu.no/en-gb/hm/Publications/Reports/2012/2012-029). The risk classification system combines a qualitative hazard assessment with a consequences assessment focusing on potential life losses. The hazard assessment is based on a series of nine geomorphological, engineering geological and structural criteria, as well as displacement rates, past events and other signs of activity. We present a method for consequence assessment comprising four main steps: 1. computation of the volume of the unstable rock slope; 2. run-out assessment based on the volume-dependent angle of reach (Fahrböschung) or detailed numerical run-out modelling; 3. assessment of possible displacement wave propagation and run-up based on empirical relations or modelling in 2D or 3D; and 4. estimation of the number of persons exposed to rock avalanches or displacement waves. Volume computation of an unstable rock slope is based on the sloping local base level technique, which uses a digital elevation model to create a second-order curved surface between the mapped extent of

  9. Development of virtual research environment for regional climatic and ecological studies and continuous education support

    NASA Astrophysics Data System (ADS)

    Gordov, Evgeny; Lykosov, Vasily; Krupchatnikov, Vladimir; Bogomolov, Vasily; Gordova, Yulia; Martynova, Yulia; Okladnikov, Igor; Titov, Alexander; Shulgina, Tamara

    2014-05-01

    measurements data and support profound statistical analysis of storaged and modeled on demand data. In particular, one can run the integrated models, preprocess modeling results data, using dedicated modules for numerical processing perform analysys and visualize obtained results. New functionality recently has been added to the statistical analysis tools set aimed at detailed studies of climatic extremes occurring in Northern Asia. The VRE element is also supporting thematic educational courses for students and post-graduate students of the Tomsk State University. In particular, it allow students to perform on-line thematic laboratory work cycles on the basics of analysis of current and potential future regional climate change using Siberia territory as an example (Gordova et al, 2013). We plan to expand the integrated models set and add comprehensive surface and Arctic Ocean description. Developed VRE element "Climate" provides specialists involved into multidisciplinary research projects with reliable and practical instruments for integrated research of climate and ecosystems changes on global and regional scales. With its help even a user without programming skills can process and visualize multidimensional observational and model data through unified web-interface using a common graphical web-browser. This work is partially supported by SB RAS project VIII.80.2.1, RFBR grant 13-05-12034, grant 14-05-00502, and integrated project SB RAS 131. References 1. Gordov E.P., Lykosov V.N., Krupchatnikov V.N., Okladnikov I.G., Titov A.G., Shulgina T.M. Computationaland information technologies for monitoring and modeling of climate changes and their consequences. Novosibirsk: Nauka, Siberian branch, 2013. - 195 p. (in Russian) 2. T.M. Shulgina, E.P. Gordov, I.G. Okladnikov, A.G., Titov, E.Yu. Genina, N.P. Gorbatenko, I.V. Kuzhevskaya,A.S. Akhmetshina. Software complex for a regional climate change analysis. // Vestnik NGU. Series: Information technologies. 2013. Vol. 11. Issue 1. P