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Sample records for solar-assisted desiccant cooling

  1. A Field-Test of Solar Assisted Adsorptive Desiccant Cooling System

    NASA Astrophysics Data System (ADS)

    Ohkura, Masashi; Kodama, Akio; Hirose, Tsutomu

    A field-test of solar assisted desiccant evaporative cooling process has been carried out, which is a quite attractive cooling / dehumidification process considering various environmental problems caused by conventional electricity driven air conditioners. The process performance has been examined by means of temperature drop between outside air and supply air and COPs (COP value based on solar irradiation). This cooling performance was strongly influenced by solar irradiation and ambient air condition. Stable irradiation produced a higher regeneration temperature resulting higher dehumidifying performance. At one day with as table solar irradiation, the cooling process could produce cool supply air of 18.7°C against the ambient air of 30.1°C and averaged COP, was 0.41. On the other hand, unstable irradiation due to some clouds made the dehumidifying performance lower. However, decrease in the cooling performance was small compared to that obtained at the stable irradiation condition. This is due to buffering by thermal storage of the water circulating in solar collectors. Influence of ambient humidity on the cooling performance was rather serious. At higher humidity condition, the amount of dehumidified water became larger due to increase of effective adsorption capacity of the desiccant rotor. However, the temperature drop was decreased to 6.9°C. This behavior was mainly due to simultaneous increase of humidity and temperature in the dehumidified air. In this situation, an effective evaporation in the following water spray evaporative cooler did not occur.

  2. Closed cycle desiccant cooling system

    NASA Astrophysics Data System (ADS)

    Tchernev, D. I.; Emerson, D. T.

    1986-10-01

    The breadboard prototype of a closed cycle desiccant cooling system was designed, constructed and its performance tested. The system combines the sorption properties of solid zeolite/refrigerant vapor pairs with the principle of regenerative heat exchangers. Since solid zeolites are difficult to move in vacuum tight containers and in order to avoid intermittent operation, the desiccant is housed in two separate containers which are alternately heated and cooled by a heat transfer fluid. Using the principle of energy regeneration, the heat removed from the container being cooled is recycled in the container being heated. The breadboard system, with 90 pounds of zeolite, demonstrated a recycling efficiency of 75%, while the system capacity was 2,000 Btu/hr. This significantly increased the system thermal Coefficient of Performance (COP) to 1.1 at ARI conditions from the single container thermal COP of 0.4.

  3. Options of desiccant cooling and dehumidification technology

    SciTech Connect

    Kweller, E. ); Mei, V.C.; Chen, F.C. )

    1991-01-01

    The recent CFC issue regarding the depleting of ozone layer and the greenhouse effect has become an impetus in research and development work for non-CFC air conditioning alternatives. Desiccant cooling is considered a good candidate for replacement of vapor compression chillers. A review of the present status of the desiccant based systems indicate that the technology has significantly advanced in recent years, and has become one of the most promising alternatives. New and better desiccants have been developed and novel cycles and more efficient system designs have been studied. As a result, the thermal coefficient of performance (COP) of desiccant cooling systems has been revised from around 0.6 to 1.0 or higher. These advances coupled with potentially reduced production cost could become very competitive with conventional systems in the near future. This study gives a review of the present status of desiccant materials, system designs, and computer models. 17 refs., 4 figs.

  4. Performance of cross-cooled desiccant dehumidifiers

    SciTech Connect

    Mei, V.C.; Lavan, Z.

    1980-01-01

    A cross-cooled silica gel desiccant dehumidifier model was designed, built and tested. The performance of the unit was studied as a function of inlet process stream dew point, process stream and cooling stream flowrates and regeneration stream temperature and dew point. The tests were also simulated by a computer program and were compared to the experimental results.

  5. Desiccant cooling using unglazed transpired solar collectors

    SciTech Connect

    Pesaran, A.A. ); Wipke, K. )

    1992-05-01

    The use of unglazed solar collectors for desiccant regeneration in a solid desiccant cooling cycle was investigated because these collectors are lower in cost than conventional glazed flat-plate collectors. Using computer models, the performance of a desiccant cooling ventilation cycle integrated with either unglazed transpired collectors or conventional glazed flat-plate collectors was obtained. We found that the thermal performance of the unglazed system was lower than the thermal performance of the glazed system because the unglazed system could not take advantage of the heat of adsorption released during the dehumidification process. For a 3-ton cooling system, although the area required for the unglazed collector was 69% more than that required for the glazed collector, the cost of the unglazed collector array was 44% less than the cost of the glazed collector array. The simple payback period of the unglazed system was half of the payback period of the glazed collector when compared to an equivalent gas-fired system. Although the use of unglazed transpired collectors makes economic sense, some practical considerations may limit their use in desiccant regeneration. 8 refs.

  6. Desiccant cooling: State-of-the-art assessment

    SciTech Connect

    Pesaran, A.A.; Penney, T.R.; Czanderna, A.W.

    1992-10-01

    The objectives of this document are to present an overview of the work accomplished to date on desiccant cooling to provide assessment of the state of the art of desiccant cooling technology in the field of desiccant material dehumidifier components, desiccant systems, and models. The report also discusses the factors that affect the widespread acceptance of desiccant cooling technology. This report is organized as follows. First, a basic description and historical overview of desiccant cooling technology is provided. Then, the recent research and development (R&D) program history (focusing on DOE`s funded efforts) is discussed. The status of the technology elements (materials, components, systems) is discussed in detail and a preliminary study on the energy impact of desiccant technology is presented. R&D needs for advancing the technology in the market are identified. The National Renewable Energy Laboratory`s unique desiccant test facilities and their typical outputs are described briefly. Finally, the results of a comprehensive literature search on desiccant cooling are presented in a bibliography. The bibliography contains approximately 900 citations on desiccant cooling.

  7. Desiccant cooling: State-of-the-art assessment

    SciTech Connect

    Pesaran, A.A.; Penney, T.R.; Czanderna, A.W.

    1992-10-01

    The objectives of this document are to present an overview of the work accomplished to date on desiccant cooling to provide assessment of the state of the art of desiccant cooling technology in the field of desiccant material dehumidifier components, desiccant systems, and models. The report also discusses the factors that affect the widespread acceptance of desiccant cooling technology. This report is organized as follows. First, a basic description and historical overview of desiccant cooling technology is provided. Then, the recent research and development (R D) program history (focusing on DOE's funded efforts) is discussed. The status of the technology elements (materials, components, systems) is discussed in detail and a preliminary study on the energy impact of desiccant technology is presented. R D needs for advancing the technology in the market are identified. The National Renewable Energy Laboratory's unique desiccant test facilities and their typical outputs are described briefly. Finally, the results of a comprehensive literature search on desiccant cooling are presented in a bibliography. The bibliography contains approximately 900 citations on desiccant cooling.

  8. Testing of novel desiccant materials and dehumidifier matrices for desiccant cooling applications

    SciTech Connect

    Pesaran, A.A.; Bingham, C.E.

    1989-03-01

    This paper presents the results of testing of desiccant materials and dehumidifier matrices for desiccant cooling and dehumidification applications. In testing desiccant materials, we used a gravimetric technique to measure the moisture capacity of four desiccant materials. These materials were microporous silica gel powder, macroporous silica gel powder, polystyrene sulfonic acid sodium salt, and a silica-gel/epoxy composite. The microporous silica gel powder had the most desirable moisture capacity properties of the four materials tested for desiccant cooling applications. The polystyrene sulfonic acid sodium salt showed some promise. Our testing of dehumidifier matrices included measuring the pressure drop and heat- and mass-transfer rate characteristics of a silica-gel/corrugated dehumidifier matrix under conditions typical of desiccant cooling systems. The matrix is a section of a commercial dehumidifier. The transient dehumidification capacity of the matrix was calculated from the tests and compared with previously tested matrices. 9 refs., 10 figs., 2 tabs.

  9. Integrated Modeling of Building Energy Requirements IncorporatingSolar Assisted Cooling

    SciTech Connect

    Firestone, Ryan; Marnay, Chris; Wang, Juan

    2005-08-10

    This paper expands on prior Berkeley Lab work on integrated simulation of building energy systems by the addition of active solar thermal collecting devices, technology options not previously considered (Siddiqui et al 2005). Collectors can be used as an alternative or additional source of hot water to heat recovery from reciprocating engines or microturbines. An example study is presented that evaluates the operation of solar assisted cooling at a large mail sorting facility in southern California with negligible heat loads and year-round cooling loads. Under current conditions solar thermal energy collection proves an unattractive option, but is a viable carbon emission control strategy.

  10. Desiccant dehumidification and cooling systems assessment and analysis

    SciTech Connect

    Collier, R.K. Jr.

    1997-09-01

    The objective of this report is to provide a preliminary analysis of the principles, sensitivities, and potential for national energy savings of desiccant cooling and dehumidification systems. The report is divided into four sections. Section I deals with the maximum theoretical performance of ideal desiccant cooling systems. Section II looks at the performance effects of non-ideal behavior of system components. Section III examines the effects of outdoor air properties on desiccant cooling system performance. Section IV analyzes the applicability of desiccant cooling systems to reduce primary energy requirements for providing space conditioning in buildings. A basic desiccation process performs no useful work (cooling). That is, a desiccant material drying air is close to an isenthalpic process. Latent energy is merely converted to sensible energy. Only when heat exchange is applied to the desiccated air is any cooling accomplished. This characteristic is generic to all desiccant cycles and critical to understanding their operation. The analyses of Section I show that desiccant cooling cycles can theoretically achieve extremely high thermal CoP`s (>2). The general conclusion from Section II is that ventilation air processing is the most viable application for the solid desiccant equipment analyzed. The results from the seasonal simulations performed in Section III indicate that, generally, the seasonal performance of the desiccant system does not change significantly from that predicted for outdoor conditions. Results from Section IV show that all of the candidate desiccant systems can save energy relative to standard vapor-compression systems. The largest energy savings are achieved by the enthalpy exchange devise.

  11. An assessment of desiccant cooling and dehumidification technology

    SciTech Connect

    Mei, V.C.; Chen, F.C. ); Lavan, Z. ); Collier, R.K. Jr. ); Meckler, G. )

    1992-07-01

    Desiccant systems are heat-actuated cooling and dehumidification technology. With the recent advances in this technology, desiccant systems can now achieve a primary energy coefficient of performance (COP) between 1.3 and 1.5, with potential to go to 1.7 and higher. It is becoming one of the most promising alternatives to conventional cooling systems. Two important and well-known advantages of desiccant cooling systems are that they are CFC free and they can reduce the electricity peak load. Another important but lesser-known advantage of desiccant technology is its potential for energy conservation. The energy impact study in this report indicated that a possible 13% energy saving in residential cooling and 8% in commercial cooling is possible. Great energy saving potential also exists in the industrial sector if industrial waste heat can be used for desiccant regeneration. The latest study on desiccant-integrated building heating, ventilating, and air conditioning (HVAC) systems indicated that the initial cost for the conventional cooling equipment was greatly reduced by using desiccant technology because of downsized compressors, fans, and ductworks. This cost reduction was more than enough to offset the cost of desiccant equipment. Besides, the system operation cost was also reduced. All these indicate that desiccant systems are also cost effective. This study provides an updated state-of-the-art assessment forsiccant technology in the field of desiccant materials, systems, computer models, and theoretical analyses. From this information the technology options were derived and the future research and development needs were identified. Because desiccant technology has already been applied in the commercial building sector with very encouraging results, it is expected that future market breakthroughs will probably start in this sector. A market analysis for the commercial building application is therefore included.

  12. Development of Membrane-Based Desiccant Fiber for Vacuum Desiccant Cooling.

    PubMed

    Yang, Yifan; Rana, Dipak; Lan, Christopher Q; Matsuura, Takeshi

    2016-06-22

    A novel hydrophobic membrane-based desiccant fiber (MDF) was developed by loading lithium chloride into hydrophobic hollow fiber membranes. The MDF thus made was then tested for vapor absorption under controlled conditions. Furthermore, an MDF pad, which was made by weaving MDF into a piece of garment, was built into a laboratory vacuum desiccant cooling (VDC) setup, which included the MDF pad as the desiccant layer and a cooling towel saturated with water as the water reservoir, to test the cooling effects at atmospheric pressure and vacuum of 25 in. of Hg. Results indicate that MDF is suitable for applications such as in VDC. Mass and heat transfer of vapor absorption by MDF were also analyzed.

  13. Development of Membrane-Based Desiccant Fiber for Vacuum Desiccant Cooling.

    PubMed

    Yang, Yifan; Rana, Dipak; Lan, Christopher Q; Matsuura, Takeshi

    2016-06-22

    A novel hydrophobic membrane-based desiccant fiber (MDF) was developed by loading lithium chloride into hydrophobic hollow fiber membranes. The MDF thus made was then tested for vapor absorption under controlled conditions. Furthermore, an MDF pad, which was made by weaving MDF into a piece of garment, was built into a laboratory vacuum desiccant cooling (VDC) setup, which included the MDF pad as the desiccant layer and a cooling towel saturated with water as the water reservoir, to test the cooling effects at atmospheric pressure and vacuum of 25 in. of Hg. Results indicate that MDF is suitable for applications such as in VDC. Mass and heat transfer of vapor absorption by MDF were also analyzed. PMID:27253330

  14. Advances in open-cycle solid desiccant cooling

    SciTech Connect

    Penney, T R; Maclaine-cross, I

    1985-05-01

    Of the solar cooling options available open cycle solid desiccant cooling looks very promising. A brief review of the experimental and analytical efforts to date shows that within the last 10 years thermal performance has doubled. Research centers have been developed to explore new materials and geometry options and to improve and validate mathematical models that can be used by design engineers to develop new product lines. Typical results from the Solar Energy Research Institute's (SERI) Desiccant Cooling Research Program are shown. Innovative ideas for new cycles and spinoff benefits provide incentives to continue research in this promising field.

  15. Solar space heating and cooling by selective use of the components of a desiccant cooling system

    NASA Astrophysics Data System (ADS)

    Abbud, Ihsan Aladdin

    The economic advantages of by-passing various components of a desiccant cooling system under conditions not requiring their use are estimated by evaluating the annual costs of heating and cooling a commercial building in three representative U.S. cities. Life-cycle costs of systems employing solar heat for space heating and desiccant regeneration are compared with those using electric heat. The costs of purchasing and operating heating and desiccant cooling systems, with and without solar heat supply, are compared with those employing conventional heating and vapor compression cooling. The conditions under which commercial buildings can be cooled with desiccant systems at costs competitive with conventional systems are identified. A commercially available vapor compression air conditioner is used as a standard of comparison for energy consumption and room comfort. Heating and cooling requirements of the building are determined by use of the BLAST computer model in a simulation of long term system operation. Performance of the desiccant cooling system and life cycle savings obtained by its use are determined by simulation employing the TRNSYS computer model. TRNSYS compatible subroutines are developed to simulate operation of the desiccant equipment, the building, and the controllers that operate and monitor the system components. The results are presented in tabular and graphical form. This study shows that in the widely different climates represented in Los Angeles, New York, and Miami, by-passing various components in the desiccant cooling system when they are not needed is economically advantageous. Operation cost of the complete system decreased by 47.3% in Los Angeles, by 30.9% in New York City, and by 23.9% in Miami by not operating the desiccant wheel and other elements. The ventilation desiccant cooling system has major economic advantage over conventional systems under conditions of moderate humidity, as in Los Angeles and New York City. In Miami, however

  16. Photovoltaic-electrodialysis regeneration method for liquid desiccant cooling system

    SciTech Connect

    Li, Xiu-Wei; Zhang, Xiao-Song

    2009-12-15

    Liquid desiccant cooling system (LDCS) is an (a novel) air-conditioning system with good energy saving potential. Regenerator is the power centre for LDCS. Currently, the regeneration process is always fuelled by thermal energy. Nevertheless, this regeneration pattern has some disadvantages in that its performance will become poor when the surrounding atmosphere is of high humidity, and the heat provided for regeneration will be unfavourable to the following dehumidification process. To ameliorate that, a new regeneration method is proposed in this paper: a membrane regenerator is employed to regenerate the liquid desiccant in an electrodialysis way; while solar photovoltaic generator is adopted to supply electric power for this process. Analysis has been made about this new regeneration method and the result reveals: this new manner achieves good stability with the immunity against the adverse impact from the outside high humidity; its performance is much higher than that of the thermal regeneration manner while putting aside the low efficiency of the photovoltaic system. Besides, purified water can be obtained in company with the regeneration process. (author)

  17. Impact of ambient pressure on performance of desiccant cooling systems

    SciTech Connect

    Pesaran, A.A.

    1991-12-01

    The impact of ambient pressure on the performance of the ventilation cycle desiccant cooling system and its components was studied using computer simulations. The impact of ambient pressure depended on whether the system was designed for fixed-mass flow rate or fixed-volume flow rate operation. As ambient pressure decreased from 1.0 to 0.8 atm, the system thermal coefficient of performance increased by 8% for both fixed-mass and fixed-volume flow rate, the cooling capacity of the system (in kW) was decreased by 14% for the fixed-volume flow rate system and increased by 7% for the fixed-mass flow rate system, the electric power requirements for the system with fixed-volume flow rate did not change, and the electric power requirement for the fixed-mass flow rate system increased by 44%. The overall coefficient of performance increased up to 5% for the fixed-volume flow rate systems, and decreased up to 4% for the fixed-mass flow rate system. 16 refs.

  18. 4-rotor Desiccant Cooling Process Equipped with a Double Stage Dehumidification

    NASA Astrophysics Data System (ADS)

    Ando, Kosuke; Kodama, Akio; Okano, Hiroshi; Asada, Toshinobu

    2-rotor desiccant cooling process consisting of a honeycomb rotor dehumidifier and a sensible heat exchanger is a mainstream of the desiccant cooling process which are practically applied to supermarket, hospital and so on. However, this process cannot produce a sufficient dehumidifying performance in high humidity region. In this study, 4-rotor desiccant cooling process equipped with a double stage dehumidification was proposed and investigated experimentally. In this process, regeneration temperature around 60°C could produce a sufficient dehumidifying performance even at high ambient humidity. “Serial heat supply” mode was considered to improve the heat efficiency by the cascade use of regeneration heat inside the cooling cycle. The dehumidifying performance of the “serial heat supply” mode was only slightly lower than that of a “parallel heat supply” mode at which the same temperature hot air was supplied to the both heaters. However, dehumidifying performance of this “serial heat supply” mode was much higher than that of conventional 2-rotor desiccant cooling process. Furthermore, the desiccant rotors of 0.1m in thickness were mounted to 4-rotor desiccant cooling process in place of the 0.2m desiccant rotors. It was found that the drop of dehumidifying performance of the process equipped with 0.1m desiccant rotors was only by 10 percents comparing with the 0.2m rotor process. Moreover, it was found that optimization of rotation speed of the desiccant rotor was needed to improve the energy efficiency. Regarding the supply point of return air, it was also found that return air should be supplied to the regeneration inlet of the second stage for higher dehumidifying performance.

  19. Research and development needs for desiccant cooling technology 1992--1997. (Supplement to the NREL report, Desiccant Cooling: State-of-the-Art Assessment)

    SciTech Connect

    Pesaran, A A

    1992-12-01

    This report is a supplement to Desiccant Cooling: State-of-the-Art Assessment (NREL/TP-254-4147, DE93000013). In this supplement document we have described a detailed program assuming sufficient funding to implement the R&D activities needed. Desiccant dehumidification is a mature technology for industrial applications, and in recent years the technology has been used for air conditioning a number of institutional and commercial buildings. Our proposal is based on argumentative discussions at various national meetings with leaders of the technology. The goal is the penetration of the broad air conditioning market. This work is funded by the Buildings technology Office of the US Department of Energy.

  20. Controlling rotary desiccant wheels for dehumidification and cooling

    SciTech Connect

    Crooks, K.W.; Banks, N.J.

    1996-12-31

    With greater focus on indoor air quality (IAQ) and ventilation, humidity control within spaces such as office buildings, hotels, schools, ice-skating rinks, nursing homes, and operating rooms has become paramount during the past decade. Control of relative humidity (RH) has been linked to increased comfort and the improved health of building occupants. The desiccant wheel process can be utilized in these applications to provide increased dehumidification while introducing minimal additional control parameters, often at lower cost.

  1. A low-cost-solar liquid desiccant system for residential cooling

    NASA Astrophysics Data System (ADS)

    Ware, Joel D., III

    The use of liquid desiccants for dehumidification of heating, ventilation, and air conditioning (HVAC) process air is becoming a more promising concept as the drive for energy conservation continues to grow. Recently, liquid desiccant systems have been implemented on the commercial level in conjunction with evaporative coolers and have recorded energy savings upwards of 50%. The aim of this research is to test the potential liquid desiccant systems have on the residential level when paired with a conventional vapor compression cycle and to construct a system that would overcome some of its barriers to the residential market. A complete low-cost-solar liquid desiccant system was designed, constructed, and tested in the Off-Grid Zero Emissions Building (OGZEB) at the Florida State University. Key design characteristics include turbulent process air flow through the conditioner and airside heating in the regenerator. The system was tested in the two following ways: (1) for the energy savings while maintaining a constant temperature over a twenty four hour period and (2) for the energy savings over a single cooling cycle. The liquid desiccant system achieved a maximum energy savings of 38% over a complete day and 52% over a single cooling cycle. It was projected that the system has the potential to save 1064 kWh over the course of a year. When combined with a renewable source of heat for regeneration, liquid desiccant systems become very cost effective. The levelized cost of energy for the combination of the liquid desiccant system and solar thermal collectors was calculated to be 7.06 C/kWh with a payback period of 4.4 years. This research provides evidence of the technology's potential on the residential sector and suggests ways for it to become competitive in the market.

  2. Research and development needs for desiccant cooling technology 1992--1997

    SciTech Connect

    Pesaran, A.A.

    1992-12-01

    This report is a supplement to Desiccant Cooling: State-of-the-Art Assessment (NREL/TP-254-4147, DE93000013). In this supplement document we have described a detailed program assuming sufficient funding to implement the R D activities needed. Desiccant dehumidification is a mature technology for industrial applications, and in recent years the technology has been used for air conditioning a number of institutional and commercial buildings. Our proposal is based on argumentative discussions at various national meetings with leaders of the technology. The goal is the penetration of the broad air conditioning market. This work is funded by the Buildings technology Office of the US Department of Energy.

  3. Energy and economic assessment of desiccant cooling systems coupled with single glazed air and hybrid PV/thermal solar collectors for applications in hot and humid climate

    SciTech Connect

    Beccali, Marco; Finocchiaro, Pietro; Nocke, Bettina

    2009-10-15

    This paper presents a detailed analysis of the energy and economic performance of desiccant cooling systems (DEC) equipped with both single glazed standard air and hybrid photovoltaic/thermal (PV/t) collectors for applications in hot and humid climates. The use of 'solar cogeneration' by means of PV/t hybrid collectors enables the simultaneous production of electricity and heat, which can be directly used by desiccant air handling units, thereby making it possible to achieve very energy savings. The present work shows the results of detailed simulations conducted for a set of desiccant cooling systems operating without any heat storage. System performance was investigated through hourly simulations for different systems and load combinations. Three configurations of DEC systems were considered: standard DEC, DEC with an integrated heat pump and DEC with an enthalpy wheel. Two kinds of building occupations were considered: office and lecture room. Moreover, three configurations of solar-assisted air handling units (AHU) equipped with desiccant wheels were considered and compared with standard AHUs, focusing on achievable primary energy savings. The relationship between the solar collector's area and the specific primary energy consumption for different system configurations and building occupation patterns is described. For both occupation patterns, sensitivity analysis on system performance was performed for different solar collector areas. Also, this work presents an economic assessment of the systems. The cost of conserved energy and the payback time were calculated, with and without public incentives for solar cooling systems. It is worth noting that the use of photovoltaics, and thus the exploitation of related available incentives in many European countries, could positively influence the spread of solar air cooling technologies (SAC). An outcome of this work is that SAC systems equipped with PV/t collectors are shown to have better performance in terms of

  4. Analysis of a field-installed hybrid solar desiccant cooling system

    SciTech Connect

    West, M.K.; Iyer, S.V.

    1995-08-01

    Many hotels, restaurants, and supermarkets report problems with high humidity. Also, heating, ventilating, and air-conditioning (HVAC) humidity issues were found to be major contributors to energy inefficiency in these applications. Desiccant cooling systems, thermally regenerated with solar heat, waste heat, natural gas, or other thermal sources, are becoming a viable air-conditioning option to control humidity. The objective of this work is to monitor and assess the performance of a commercial hybrid solar solid desiccant air-conditioning system in a high-humidity restaurant application. Air is heated in the regeneration section by a heat recirculator coil, a condenser waste heat recovery coil, and a solar coil, accounting for roughly 27%, 24%, and 49% of the regeneration heat, respectively, when solar energy is available. The specific humidity change across the desiccant wheel varies from 4 to 90 gr/lb (0.57 to 12.86 g/kg) over the 108 F to 197 F (42.2 C to 91.7 C) regeneration temperature range. The coefficient of performance (COP), defined as the usable cooling capacity divided by the heat input, increased from 0.2 to 1.0 at the rate of 0.01 for every 10 F rise in the regeneration temperature. The results from this project provide much valued field data as well as a design future work.

  5. Double-stage Dehumidification achieved in 2-rotor Desiccant Cooling Process

    NASA Astrophysics Data System (ADS)

    Asada, Toshinobu; Kodama, Akio

    A multi-divided rotary adsorber was proposed to realize a double-stage dehumidification in 2-rotor desiccant cooling process. In this process, once dehumidified and cooled air was dehumidified again at the different adsorption zone of the same desiccant rotor. Four process flow configurations were experimentally tested for investigations of the appropriate outside air inlet position and rotating directions of the rotary adsorber and rotary heat exchanger. It was found that dehumidifying performance was strongly influenced by the supply position of outside air to the adsorbent rotor. Humid outside air should be supplied to the latter half of the adsorption zone and once dehumidified air should be sent to the first half of the adsorption zone. This is because the increase in the amount adsorbed at the first half of the adsorption step resulted in a poor adsorbabilty at the latter half of the adsorption step for the once dehumidified dry air. Therefore, the effective use of the adsorbent rotor could be done by considering the angular distribution of the amount of adsorbed. Consequently, 30% larger amount of dehumidification than that of conventional processes under a humid summer condition was confirmed. Regarding the product air temperature, the process configurations at which secondary dehumidified air was cooled at the first half of the sensible rotor produced roughly 5 °C lower temperature air than that of the other. This implied that the directions of rotation of the adsorbent rotor and the rotary heat exchanger, or dehumidification - cooling sequence, also had a great impact on the product air condition.

  6. Effective Operation for an Adsorptive Desiccant Cooling Process using a Double-Stage Regeneration of Honeycomb Rotary Dehumidifier

    NASA Astrophysics Data System (ADS)

    Kodama, Akio; Ando, Kouke; Hirose, Tsutomu; Goto, Motonobu; Tuziguchi, Takuya; Okano, Hiroshi

    A double-stage regeneration concept has been applied to a rotary dehumidifier to achieve a high efficient desiccant cooling process. In this concept, regeneration zone of the dehumidifier was divided into two zones. One was pre-regeneration zone where the desiccant rotor was regenerated by warm air heated at the sensible heat exchanger, and the other was heating regeneration zone where the rotor was further regenerated by hot air heated up to the controlled temperature at a heating system. In this paper, the influence of the area ratio of these two zones on the dehumidifying/cooling performance and energy efficiency of this desiccant system were mainly discussed at several levels of the regeneration temperature, supply air velocity and inlet air humidity. Experimental results indicated that the area ratio of the heating regeneration zone should be optimized to produce a sufficient dehumidifying/cooling performance with high energy efficiency, considering humidity requested in supply air. It was also found that the amount of energy input was rather decreased than the decrease of the cooling performance as the area ratio of the heating regeneration zone decreased, and this tendency was more remarkable at higher regeneration temperature. Finally, the double-stage regeneration was confirmed to be an effective operating method for the desiccant cooling process equipped with a otary dehumidifier.

  7. Moving Advanced Desiccant Materials into Mainstream Non-CFC Cooling Products

    SciTech Connect

    Sand, J R; Grossman, G; Rice, C K; Fairchild, P D; Gross, I L

    1994-01-01

    Desiccant air-conditioning systems can be used as alternatives for conventional air-conditioning equipment in any commercial or residential building. Recent breakthroughs in desiccant materials technology and the creation of new markets by Indoor Air Quality issues make desiccant-based air-conditioning equipment practical for many space-conditioning applications.

  8. Economic and technical assessment of the desiccant wheel effect on the thermal performance of cross flow cooling towers in variable wet bulb temperature

    NASA Astrophysics Data System (ADS)

    Banooni, Salem; Chitsazan, Ali

    2014-05-01

    Performance improvements of cross flow cooling towers in variable wet bulb temperature were performed. A conventional mathematical model is used to predict desiccant wheel effect on the performance of cooling tower. It is found that by using optimum parameters of desiccant wheel, the inlet air wet bulb temperature into the cooling tower would decrease more than 6 °C and outlet water temperature would decrease more than 4 °C.

  9. Solar-Assisted Hemodialysis

    PubMed Central

    Agar, John W. M.; Perkins, Anthony; Tjipto, Alwie

    2012-01-01

    Summary Background and objectives Hemodialysis resource use—especially water and power, smarter processing and reuse of postdialysis waste, and improved ecosensitive building design, insulation, and space use—all need much closer attention. Regarding power, as supply diminishes and costs rise, alternative power augmentation for dialysis services becomes attractive. The first 12 months of a solar-assisted dialysis program in southeastern Australia is reported. Design, setting, participants, & measurements A 24-m2, 3-kWh rated solar array and inverter—total cost of A$16,219—has solar-assisted the dialysis-related power needs of a four-chair home hemodialysis training service. All array-created, grid-donated power and all grid-drawn power to the four hemodialysis machines and minireverse osmosis plant pairings are separately metered. After the grid-drawn and array-generated kilowatt hours have been billed and reimbursed at their respective commercial rates, financial viability, including capital repayment, can be assessed. Results From July of 2010 to July of 2011, the four combined equipment pairings used 4166.5 kWh, 9% more than the array-generated 3811.0 kWh. Power consumption at 26.7 c/kWh cost A$1145.79. Array-generated power reimbursements at 23.5 c/kWh were A$895.59. Power costs were, thus, reduced by 76.5%. As new reimbursement rates (60 c/kWh) take effect, system reimbursements will more than double, allowing both free power and potential capital pay down over 7.7 years. With expected array life of ∼30 years, free power and an income stream should accrue in the second and third operative decades. Conclusions Solar-assisted power is feasible and cost-effective. Dialysis services should assess their local solar conditions and determine whether this ecosensitive power option might suit their circumstance. PMID:22223614

  10. Anisotropic stress accumulation in cooling lava flows and resulting fracture patterns: Insights from starch-water desiccation experiments

    NASA Astrophysics Data System (ADS)

    Lodge, Robert W. D.; Lescinsky, David T.

    2009-09-01

    Desiccation of starch-water slurries is a useful analog for the production of polygonal fractures/columnar joints in cooling lava flows. When left to dry completely, a simple mixture of 1:1 starch and water will produce columns that appear remarkably similar to natural columnar joints formed in cooled lava flows. Columns form when the accumulation of isotropic stress exceeds the tensile strength of a material, at which point a fracture forms and advances through the material perpendicular to the desiccating surface. Individual fractures will initially form orthogonal to the desiccation surface but will quickly evolve into a hexagonal fracture network that advances incrementally through the material. However, some fracture patterns found within natural lava flows are not hexagonal ( Lodge and Lescinsky, 2009-this issue), but rather have fracture lengths that are much longer than the distance to adjacent fractures. These fractures are commonly found at lava flows that have interacted with glacial ice during emplacement. The purpose of this study is to utilize starch analog experiments to better understand the formation of these fractures and the stress regimes responsible for their non-hexagonal patterns. To simulate anisotropic conditions during cooling, the starch slurry was poured into a container with a movable wall that was attached to a screw-type jack. The jack was then set to slowly extend or retract while the slurry desiccated. This resulted in either a decrease or increase in the chamber cross-sectional area thus creating compressional or extensional regimes. Decreasing chamber area (DCA) experiments resulted in fractures with larger lengths parallel to the direction of wall movement (also direction of compression). It also caused localized thrust faulting and curved column development. Increasing chamber area (ICA) experiments produced a zone of horizontal column development along the expanding margin (produced when the wall detached from the sample

  11. Seminar 14 - Desiccant Enhanced Air Conditioning: Desiccant Enhanced Evaporative Air Conditioning (Presentation)

    SciTech Connect

    Kozubal, E.

    2013-02-01

    This presentation explains how liquid desiccant based coupled with an indirect evaporative cooler can efficiently produce cool, dry air, and how a liquid desiccant membrane air conditioner can efficiently provide cooling and dehumidification without the carryover problems of previous generations of liquid desiccant systems. It provides an overview to a liquid desiccant DX air conditioner that can efficiently provide cooling and dehumidification to high latent loads without the need for reheat, explains how liquid desiccant cooling and dehumidification systems can outperform vapor compression based air conditioning systems in hot and humid climates, explains how liquid desiccant cooling and dehumidification systems work, and describes a refrigerant free liquid desiccant based cooling system.

  12. Moving Advanced Desiccant Materials into Mainstream Non-CFC Cooling Products

    SciTech Connect

    Sand, J. R.; Grossman, T.; Rice, C. K.; Fairchild, P. D.; Gross, I. L.

    2004-12-30

    Desiccant dehumidification technology is emerging as a technically viable alternative for comfort conditioning in many commercial and institutional buildings. Attempts to improve the indoor air quality of buildings has resulted in increasingly stringent guidelines for occupant outdoor air ventilation rates. Additionally, revised building heating, ventilating, and air-conditioning (HVAC) design criteria based on regional peak dew point data highlight the important of the latent (moisture removal) building load relative to the sensible (temperature) building load.

  13. The performance of a solar-regenerated open-cycle desiccant bed grain cooling system

    SciTech Connect

    Ismail, M.Z.; Angus, D.E. ); Thorpe, G.R. )

    1991-01-01

    The cooling of stored food grains suppresses the growth of populations of insect pests, inhibits spoilage by fungi and helps to preserve grain quality. In temperate and subtropical climates, grains may be effectively cooled by ventilating them with ambient air. In tropical climates, the enthalpy of the air must be reduced before it can be used for cooling grain. One method of achieving this is to isothermally reduce the humidity of the air. This paper describes experiments carried out on a simple-to-build solar-regenerated open-cycle grain cooling system. The device consists of a 5.85 m{sup 2} collector coupled with two beds of silica gel. Results from a series of experiments suggest that the device may be used to cool up to 200 tons of grain. The electrical power consumption of the device is of the order of 0.3 watt per ton of grain cooled, and the total electrical energy consumption is of the order of 0.7 kWh per ton of grain stored for a six-month period. The effectiveness of the device is a function of air flow rate and the enthalpy of ambient air, and results presented in this paper suggest that the solar cooling device is particularly effective in tropical climates.

  14. Analysis of the adsorption process and of desiccant cooling systems: a pseudo- steady-state model for coupled heat and mass transfer. [DESSIM, DESSIM2, DESSIM4

    SciTech Connect

    Barlow, R.S.

    1982-12-01

    A computer model to simulate the adiabatic adsorption/desorption process is documented. Developed to predict the performance of desiccant cooling systems, the model has been validated through comparison with experimental data for single-blow adsorption and desorption. A literature review on adsorption analysis, detailed discussions of the adsorption process, and an initial assessment of the potential for performance improvement through advanced component development are included.

  15. Energy and economic performance analysis of an open cycle solar desiccant dehumidification air-conditioning system for application in Hong Kong

    SciTech Connect

    Li, Yutong; Lu, Lin; Yang, Hongxing

    2010-12-15

    In this article, a transient simulation model and the EnergyPlus were used to study the energy performance and economical feasibility for integrating a solar liquid desiccant dehumidification system with a conventional vapor compression air-conditioning system for the weather condition of Hong Kong. The vapor compression system capacity in the solar assisted air-conditioning system can be reduced to 19 kW from original 28 kW of a conventional air-conditioning system as a case study due to the solar desiccant cooling. The economical performance of the solar desiccant dehumidification system is compared with that of the conventional air-conditioning system. The results show that the energy saving potentials due to incorporation of the solar desiccant dehumidification system in a traditional air-conditioning system is significant for the hot wet weather in Hong Kong due to higher COP resulted from higher supply chilled water temperature from chiller plants. The annual operation energy savings for the hybrid system is 6760 kWh and the payback period of the hybrid system is around 7 years. The study shows that the solar assisted air-conditioning is a viable technology for utilizations in subtropical areas. (author)

  16. Advanced desiccant materials research

    SciTech Connect

    Czanderna, A.W.; Thomas, T.M.

    1986-05-01

    The long-range goal of this task is to understand the role of surface phenomena in desiccant cooling materials. The background information includes a brief introduction to desiccant cooling systems (DCS) and the role of the desiccant as a system component. The purpose, background, rationale, and long-term technical approach for studying advanced desiccant materials are then treated. Experimental methods for measuring water vapor sorption by desiccants are described, and the rationale is then given for choosing a quartz crystal microbalance (QCM) for measuring sorption isotherms, rates, and cyclic stability. Background information is given about the QCM, including the quartz crystal resonator itself, the support structure for the quartz crystal, and the advantages and limitations of a QCM. The apparatus assembled and placed into operation during CY 1985 is described. The functions of the principal components of the equipment, i.e., the QCM, vacuum system, pressure gauges, residual gas analyzer, constant temperature bath, and data acquisition system, are described as they relate to the water vapor sorption measurements now under way. The criteria for narrowing the potential candidates as advanced desiccant materials for the initial studies are given. Also given is a list of 20 principal candidate materials identified based on the criteria and data available in the literature.

  17. Solar-regenerated desiccant dehumidification

    NASA Astrophysics Data System (ADS)

    Haves, P.

    1982-02-01

    The dehumidification requirements of buildings are discussed, and the most suitable desiccant material is identified as silica gel. Several conceptual designs for solar regenerated desiccant dehumidifiers using a solid desiccant are described. The construction and operation of a laboratory experiment to determine the performance of a packed bed of silica gel at low flow rate is described. The experimental results are presented and compared to the predictions of a simple computer model which assumes local equilibrium between the desiccant and the airstream. The simulations used to predict desiccant bed performance and the integration of the desiccant bed simulation with a simulation of the thermal performance of a passively cooled residence are described. Results for an average July day are presented. Sizing relationships derived from the simulation are described, and an economic analysis and recommendations for further work are presented.

  18. SERI Desiccant Cooling Test Facility. Status report. Preliminary data on the performance of a rotary parallel-passage silica-gel dehumidifier

    SciTech Connect

    Schultz, K.J.

    1986-04-01

    This report describes the SERI Desiccant Cooling Test Facility. The facility can test bench-scale rotary dehumidifiers over a wide range of controlled conditions. We constructed and installed in the test loop a prototype parallel-passage rotary dehumidifier that has spirally wound polyester tape coated with silica gel. The initial tests gave satisfactory results indicating that approximately 90% of the silica gel was active and the overall Lewis number of the wheel was near unity. The facility has several minor difficulties including an inability to control humidity satisfactorily and nonuniform and highly turbulent inlet velocities. To completely validate the facility requires a range of dehumidifier designs. Several choices are available including constructing a second parallel-passage dehumidifier with the passage spacing more uniform.

  19. Solar-assisted low energy dwellings

    SciTech Connect

    Esbensen, T V

    1980-02-01

    The Zero Energy House Group was formed as a subproject of the CCMS Solar Energy Pilot Study in 1974 by seven participating countries experimenting with solar-assisted low-energy dwellings for temperate and northern European climatic conditions. A Zero Energy House is one in which solar energy is used to meet the reduced energy needs of buildings incorporating various thermal energy conservation features. This final report of the Zero Energy House Group includes brief descriptions of 13 major low-energy dwellings in the participating CCMS countries. An overall assessment of the state-of-the-art in solar-assisted low-energy dwellings is also included.

  20. Solar-Assisted Oxidation of Toxic Cyanide

    NASA Technical Reports Server (NTRS)

    Byvik, C. E.; Miles, A.

    1985-01-01

    In solar-assisted oxidation technique, oxygen-bearing air bubbled through cyanide solution in which platinized powdered TiO2 is suspended. Light from either artifical source or natural Sunlight irradiates. Experiments demonstrated this technique effective in reducing concentration of cyanide to levels well below those achieved by other methods. Results suggest effective and inexpensive method for oxidizing cyanide in industrial wastewaters.

  1. Desiccant contamination research: Report on the desiccant contamination test facility

    SciTech Connect

    Pesaran, A.A.; Bingham, C.E.

    1991-07-01

    The activity in the cooling systems research involves research on high performance dehumidifiers and chillers that can operate efficiently with the variable thermal outputs and delivery temperatures associated with solar collectors. It also includes work on advanced passive cooling techniques. This report describes the work conducted to improve the durability of solid desiccant dehumidifiers by investigating the causes of degradation of desiccant materials from airborne contaminants and thermal cycling. The performance of a dehumidifier strongly depends on the physical properties and durability of the desiccant material. To make durable and reliable dehumidifiers, an understanding is needed of how and to what degree the performance of a dehumidifier is affected by desiccant degradation. This report, an account of work under Cooling Systems Research, documents the efforts to design and fabricate a test facility to investigate desiccant contamination based on industry and academia recommendations. It also discusses the experimental techniques needed for obtaining high-quality data and presents plans for next year. Researchers of the Mechanical and Industrial Technology Division performed this work at the Solar Energy Research Institute in FY 1988 for DOE's Office of Solar Heat Technologies. 7 refs., 19 figs., 1 tab.

  2. Experiments on sorption hysteresis of desiccant materials

    SciTech Connect

    Pesaran, A.; Zangrando, F.

    1984-08-01

    Solid desiccant cooling systems take advantage of solar energy for air conditioning. The process involves passing air through a desiccant bed for drying and subsequent evaporative cooling to provide the air conditioning. The desiccant is then regenerated with hot air provided by a gas burner or solar collectors. This performance is limited by the capacity of the desiccant, its sorption properties, and the long-term stability of the desiccant material under cyclic operation conditions. Therefore, we have developed a versatile test facility to measure the sorption properties of candidate solid desiccant materials under dynamic conditions, under different geometrical configurations, and under a broad range of process air stream conditions, characteristic of desiccant dehumidifer operation. We identified a dependence of the sorption processes on air velocity and the test cell aspect ratio and the dynamic hysteresis between adsorption and desorption processes. These experiments were geared to provide data on the dynamic performance of silica gel in a parallel-passage configuration to prepare for tests with a rotary dehumidifier that will be conducted at SERI in late FY 1984. We also recommend improving the accuracy of the isotopic perturbation technique.

  3. Solar Assisted Ground Source Heat Pump Performance in Nearly Zero Energy Building in Baltic Countries

    NASA Astrophysics Data System (ADS)

    Januševičius, Karolis; Streckienė, Giedrė

    2013-12-01

    In near zero energy buildings (NZEB) built in Baltic countries, heat production systems meet the challenge of large share domestic hot water demand and high required heating capacity. Due to passive solar design, cooling demand in residential buildings also needs an assessment and solution. Heat pump systems are a widespread solution to reduce energy use. A combination of heat pump and solar thermal collectors helps to meet standard requirements and increases the share of renewable energy use in total energy balance of country. The presented paper describes a simulation study of solar assisted heat pump systems carried out in TRNSYS. The purpose of this simulation was to investigate how the performance of a solar assisted heat pump combination varies in near zero energy building. Results of three systems were compared to autonomous (independent) systems simulated performance. Different solar assisted heat pump design solutions with serial and parallel solar thermal collector connections to the heat pump loop were modelled and a passive cooling possibility was assessed. Simulations were performed for three Baltic countries: Lithuania, Latvia and Estonia.

  4. Zero Carryover Liquid-Desiccant Air Conditioner for Solar Applications: Preprint

    SciTech Connect

    Lowenstein, A.; Slayzak, S.; Kozubal, E.

    2006-07-01

    A novel liquid-desiccant air conditioner that dries and cools building supply air will transform the use of direct-contact liquid-desiccant systems in HVAC applications, improving comfort, air quality, and providing energy-efficient humidity control.

  5. Polymers as advanced materials for desiccant applications, 1988

    SciTech Connect

    Czanderna, A.W.; Neidlinger, H.H.

    1990-09-01

    This report documents work to identify a next-generation, low-cost material with which solar energy or heat from another low-cost energy source can be used for regenerating the water vapor sorption activity of the desiccant. The objective of the work is to determine how the desired sorption performance of advanced desiccant materials can be predicted by understanding the role of the material modifications and material surfaces. The work concentrates on solid materials to be used for desiccant cooling systems and which process water vapor in an atmosphere to produce cooling. The work involved preparing modifications of polystyrene sulfonic acid sodium salt, synthesizing a hydrogel, and evaluating the sorption performances of these and similar commercially available polymeric materials; all materials were studied for their potential application in solid commercial desiccant cooling systems. Background information is also provided on desiccant cooling systems and the role of a desiccant material within such a system, and it includes the use of polymers as desiccant materials. 31 refs., 16 figs., 5 tabs.

  6. Breakthrough Video: Desiccant Enhanced Evaporative Air Conditioning

    SciTech Connect

    2012-01-01

    Researchers at the National Renewable Energy Laboratory (NREL) invented a breakthrough technology that improves air conditioning in a novel way—with heat. NREL combined desiccant materials, which remove moisture from the air using heat, and advanced evaporative technologies to develop a cooling unit that uses 90% less electricity and up to 80% less total energy than traditional air conditioning (AC). This solution, called the desiccant enhanced evaporative air conditioner (DEVAP), also controls humidity more effectively to improve the comfort of people in buildings.

  7. Experimental evaluation of commercial desiccant dehumidifier wheels

    SciTech Connect

    Slayzak, S.J.; Pesaran, A.A.; Hancock, C.E.

    1996-01-01

    The National Renewable Energy Laboratory is currently characterizing the state-of-the-art in desiccant dehumidifiers, the key component of desiccant cooling systems. The data are being obtained in our HVAC Equipment Test Facility in accordance with the proposed ASHRAE test standard. The experimental data will provide industry and end users with independent performance evaluation and the United States Department of Energy and NREL with the information necessary to assess advances in the energy savings potential of the technology. This paper proposes several figures of merit for evaluating performance. The results of these tests indicate that dehumidification capacity performance parameters can be correlated to process inlet air relative humidity.

  8. Active Desiccant-Based Preconditioning Market Analysis and Product Development

    SciTech Connect

    Fischer, J.

    2001-01-11

    The Phase 1 report (ORNL/Sub/94-SVO44/1), completed earlier in this program, involved a comprehensive field survey and market analysis comparing various specialized outdoor air handling units. This initial investigation included conventional cooling and reheat, conventional cooling with sensible recovery, total energy recovery systems (passive desiccant technology) and various active desiccant systems. The report concluded that several markets do promise a significant sales opportunity for a Climate Changer-based active desiccant system offering. (Climate Changer is a registered trademark of Trane Company.) This initial market analysis defined the wants and needs of the end customers (design engineers and building owners), which, along with subsequent information included in this report, have been used to guide the determination of the most promising active desiccant system configurations. This Phase 2 report begins with a summary of a more thorough investigation of those specific markets identified as most promising for active desiccant systems. Table 1 estimates the annual sales potential for a cost-effective product line of active desiccant systems, such as that built from Climate Changer modules. The Product Development Strategy section describes the active desiccant system configurations chosen to best fit the needs of the marketplace while minimizing system options. Key design objectives based on market research are listed in this report for these active desiccant systems. Corresponding performance goals for the dehumidification wheel required to meet the overall system design objectives are also defined. The Performance Modeling section describes the strategy used by SEMCO to design the dehumidification wheels integrated into the prototype systems currently being tested as part of the U.S. Department of Energy's Advanced Desiccant Technology Program. Actual performance data from wheel testing was used to revise the system performance and energy analysis

  9. Desiccant Enhanced Evaporative Air Conditioning: Parametric Analysis and Design; Preprint

    SciTech Connect

    Woods, J.; Kozubal, E.

    2012-10-01

    This paper presents a parametric analysis using a numerical model of a new concept in desiccant and evaporative air conditioning. The concept consists of two stages: a liquid desiccant dehumidifier and a dew-point evaporative cooler. Each stage consists of stacked air channel pairs separated by a plastic sheet. In the first stage, a liquid desiccant film removes moisture from the process (supply-side) air through a membrane. An evaporatively-cooled exhaust airstream on the other side of the plastic sheet cools the desiccant. The second-stage indirect evaporative cooler sensibly cools the dried process air. We analyze the tradeoff between device size and energy efficiency. This tradeoff depends strongly on process air channel thicknesses, the ratio of first-stage to second-stage area, and the second-stage exhaust air flow rate. A sensitivity analysis reiterates the importance of the process air boundary layers and suggests a need for increasing airside heat and mass transfer enhancements.

  10. Solar assisted heat pump for a swine nursery barn

    SciTech Connect

    Havard, P.L.

    1981-01-01

    The raising of hogs in Canada and Northern United States may require heating year round in the nursery area of the operation. The use of a solar assisted heat pump system can lead to substantial energy savings. The heat system and the computer simulation output for a demonstration project built in this area are summarized.

  11. Degradation of desiccants upon contamination: An experimental study

    SciTech Connect

    Pesaran, A A

    1990-11-01

    Experiments were conducted to quantify the effects of thermal cycling and exposure to contamination on solid desiccant materials that may be used in desiccant cooling systems. A test apparatus was used to thermally cycle several desiccant samples and expose them to ambient or contaminated humid air. The source of contamination was cigarette smoke. Six different solid desiccants were tested: two types of silica gel, activated alumina, activated carbon, molecular sieves, and lithium chloride. The exposed desiccant samples were removed after 0.5, 1, 2, 4, or 11 months of exposure and their moisture capacities were measured. Other tests were conducted to characterize pollutants deposited on the exposed samples or to evaluate impact of exposure on internal structure of the samples. Compared to fresh samples, the capacity loss due to thermal cycling with ambient air was generally 10% to 30%. The capacity loss due to only cigarette smoke was generally between 20% to 50%. 7 refs., 8 figs., 3 tabs.

  12. An economic analysis of a solar open cycle desiccant dehumidification system

    SciTech Connect

    Thornbloom, M.; Nimmo, B.

    1995-11-01

    Desiccants can be used to remove moisture from an air stream prior to sensible cooling. This may lead to a significant reduction in total air conditioning load. In addition, solar thermal energy can be used to regenerate the desiccant and thereby further increase the energy savings. This paper compares a solar-regenerated Open Cycle Liquid Desiccant Dehumidification System to a Conventional (vapor compression) System by comparing performance and costs. The liquid desiccant selected for the study is a solution of calcium chloride and water. The desiccant solution in the glazed collector/ regenerator comes in direct contact with the ambient air. The use of conventional materials and roofing construction methods reduces costs.

  13. How worms survive desiccation

    PubMed Central

    Erkut, Cihan; Penkov, Sider; Fahmy, Karim; Kurzchalia, Teymuras V.

    2012-01-01

    While life requires water, many organisms, known as anhydrobiotes, can survive in the absence of water for extended periods of time. Although discovered 300 years ago, we know very little about the fascinating phenomenon of anhydrobiosis. In this paper, we summarize our previous findings on the desiccation tolerance of the Caenorhabditis elegans dauer larva. A special emphasis is given to the role of trehalose in protecting membranes against desiccation. We also propose a simple mechanism for this process. PMID:24058825

  14. Polymers as advanced materials for desiccant applications

    SciTech Connect

    Czanderna, A.W.

    1990-12-01

    This research is concerned with solid materials used as desiccants for desiccant cooling systems (DCSs) that process water vapor in an atmosphere to produce cooling. Background information includes an introduction to DCSs and the role of the desiccant as a system component. The water vapor sorption performance criteria used for screening the modified polymers prepared include the water sorption capacity from 5% to 80% relative humidity (R.H.), isotherm shape, and rate of adsorption and desorption. Measurements are presented for the sorption performance of modified polymeric advanced desiccant materials with the quartz crystal microbalance. Isotherms of polystyrene sulfonic acid (PSSA) taken over a 5-month period show that the material has a dramatic loss in capacity and that the isotherm shape is time dependent. The adsorption and desorption kinetics for PSSA and all the ionic salts of it studied are easily fast enough for commercial DCS applications with a wheel rotation speed of 6 min per revolution. Future activities for the project are addressed, and a 5-year summary of the project is included as Appendix A. 34 refs., 20 figs., 3 tabs.

  15. Second generation ground coupled solar assisted heat pump systems. Six month progress report

    SciTech Connect

    Rhodes, G W; Backlund, J C; Helm, J M

    1981-01-01

    Progress is reported on an investigation of the technical and commercial viability of a novel ground coupled, solar assisted heat pump system for residential space heating and cooling applications. Specific areas of study are solar collector/heat rejector performance, flat plate earth heat exchanger performance, system performance simulations, and commercialization and marketing analysis. Collector/rejector performance, determined by various thermal experiments, is discussed. The design and construction of an experimental site to study ground coupling is discussed. Theoretical analysis is also presented. The performance of the GCSAHP system and conventional alternatives, as determined by simple computer models, is presented and discussed. Finally, the commercial viability of this unique space conditioning system is examined.

  16. Solar-Assisted Electric Vehicle Charging Station Interim Report

    SciTech Connect

    Lapsa, Melissa Voss; Durfee, Norman; Maxey, L Curt; Overbey, Randall M

    2011-09-01

    Oak Ridge National Laboratory (ORNL) has been awarded $6.8 million in the Department of Energy (DOE) American Recovery and Reinvestment Act (ARRA) funds as part of an overall $114.8 million ECOtality grant with matching funds from regional partners to install 125 solar-assisted Electric Vehicle (EV) charging stations across Knoxville, Nashville, Chattanooga, and Memphis. Significant progress has been made toward completing the scope with the installation of 25 solar-assisted charging stations at ORNL; six stations at Electric Power Research Institute (EPRI); and 27 stations at Nissan's Smyrna and Franklin sites, with three more stations under construction at Nissan's new lithium-ion battery plant. Additionally, the procurement process for contracting the installation of 34 stations at Knoxville, the University of Tennessee Knoxville (UTK), and Nashville sites is underway with completion of installation scheduled for early 2012. Progress is also being made on finalizing sites and beginning installations of 30 stations in Nashville, Chattanooga, and Memphis by EPRI and Tennessee Valley Authority (TVA). The solar-assisted EV charging station project has made great strides in fiscal year 2011. A total of 58 solar-assisted EV parking spaces have been commissioned in East and Middle Tennessee, and progress on installing the remaining 67 spaces is well underway. The contract for the 34 stations planned for Knoxville, UTK, and Nashville should be underway in October with completion scheduled for the end of March 2012; the remaining three Nissan stations are under construction and scheduled to be complete in November; and the EPRI/TVA stations for Chattanooga, Vanderbilt, and Memphis are underway and should be complete by the end of March 2012. As additional Nissan LEAFs are being delivered, usage of the charging stations has increased substantially. The project is on course to complete all 125 solar-assisted EV charging stations in time to collect meaningful data by the

  17. Experimental results on advanced rotary desiccant dehumidifiers

    SciTech Connect

    Bharathan, D; Parsons, J; Maclaine-cross, I

    1986-08-01

    The Solar Energy Research Institute (SERI) has developed the Cyclic Test Facility (CTF) to develop and validate analytical methods for evaluating and predicting the performance of advanced rotary dehumidifiers. This paper describes the CTF, the dehumidifiers tested at the CTF, and the analytical methods used. The results reported provide an engineering data base and a design tool for evaluating rotary dehumidifiers for desiccant cooling applications.

  18. Solar-assisted heat pumps and alternative-energy-awareness demonstration. Final report

    SciTech Connect

    Wetzel, O.K. Jr.

    1981-09-11

    The water to air solar assisted heat pump installation is added to an existing service/training center in Dallas, Texas. The design uses three separate water systems. The first consists of the solar collectors, circulating pump, draindown tank, heat exchanger, attendant piping and controls. The second is a closed loop system consisting of an insulated buried steel tank, an above ground expansion tank, the loop water circulating pump, the backup gas boiler, and the cooling tower. It serves six heat pumps. The third system is an open system for the cooling tower, consisting of a pump, spray heads in the cooling tower. The operation of each of the three systems is described. The system's thermodynamic efficiency is discussed, as well as a solar domestic hot water system. System performance is found to be much less than expected, with a solar fraction estimated at 30% as opposed to an expected 80%. A self-teaching solar awareness demonstration in the building is described. (LEW)

  19. Characterization of a starch based desiccant wheel dehumidifier

    NASA Astrophysics Data System (ADS)

    Beery, Kyle Edward

    Starch, cellulose, and hemicellulose have an affinity for water, and adsorb water vapor from air. Materials made from combinations of these biobased sugar polymers also have been found to possess adsorptive properties. An interesting possible application of these starch-based adsorbents is the desiccant wheel dehumidifier. The desiccant wheel dehumidifier is used in conjunction with a standard air conditioning system. In this process, ambient air is passed through a stationary section while a wheel packed with desiccant rotates through that section. The desiccant adsorbs humidity (latent load) from the air, and the air conditioning system then cools the air (sensible load). Several starch based adsorbents were developed and tested for adsorptive capacity in a new high throughput screening system. The best formulations from the high throughput screening system, also taking into account economic considerations and structural integrity, were considered for use in the desiccant wheel dehumidifier. A suitable adsorbent was chosen and formulated into a matrix structure for the desiccant wheel system. A prototype desiccant wheel system was constructed and the performance was investigated under varying regeneration temperatures and rotation speeds. The results from the experiments showed that the starch based desiccant wheel dehumidification system does transfer moisture from the inlet process stream to the outlet regeneration stream. The DESSIM model was modified for the starch based adsorbent and compared to the experimental results. Also, the results when the wheel parameters were varied were compared to the predicted results from the model. The results given by the starch based desiccant wheel system show the desired proof of concept.

  20. Design of a test facility for gas-fired desiccant-based air conditioning systems

    SciTech Connect

    Jalalzadeh-Azar, A.A.; Steele, W.G.; Hodge, B.K.

    1996-12-31

    The design of a facility for testing desiccant-based air conditioning systems is presented. The determination of the performance parameters of desiccant systems is discussed including moisture removal capacity, latent and total cooling capacities, and efficiency indexes. The appropriate procedures and key measurements for determining these parameters are identified using uncertainty analysis.

  1. Desiccation tolerance of prokaryotes.

    PubMed Central

    Potts, M

    1994-01-01

    The removal of cell-bound water through air drying and the addition of water to air-dried cells are forces that have played a pivotal role in the evolution of the prokaryotes. In bacterial cells that have been subjected to air drying, the evaporation of free cytoplasmic water (Vf) can be instantaneous, and an equilibrium between cell-bound water (Vb) and the environmental water (vapor) potential (psi wv) may be achieved rapidly. In the air-dried state some bacteria survive only for seconds whereas others can tolerate desiccation for thousands, perhaps millions, of years. The desiccated (anhydrobiotic) cell is characterized by its singular lack of water--with contents as low as 0.02 g of H2O g (dry weight)-1. At these levels the monolayer coverage by water of macromolecules, including DNA and proteins, is disturbed. As a consequence the mechanisms that confer desiccation tolerance upon air-dried bacteria are markedly different from those, such as the mechanism of preferential exclusion of compatible solutes, that preserve the integrity of salt-, osmotically, and freeze-thaw-stressed cells. Desiccation tolerance reflects a complex array of interactions at the structural, physiological, and molecular levels. Many of the mechanisms remain cryptic, but it is clear that they involve interactions, such as those between proteins and co-solvents, that derive from the unique properties of the water molecule. A water replacement hypothesis accounts for how the nonreducing disaccharides trehalose and sucrose preserve the integrity of membranes and proteins. Nevertheless, we have virtually no insight into the state of the cytoplasm of an air-dried cell. There is no evidence for any obvious adaptations of proteins that can counter the effects of air drying or for the occurrence of any proteins that provide a direct and a tangible contribution to cell stability. Among the prokaryotes that can exist as anhydrobiotic cells, the cyanobacteria have a marked capacity to do so. One

  2. Desiccant Enhanced Evaporative Air-Conditioning (DEVap): Evaluation of a New Concept in Ultra Efficient Air Conditioning

    SciTech Connect

    Kozubal, E.; Woods, J.; Burch, J.; Boranian, A.; Merrigan, T.

    2011-01-01

    NREL has developed the novel concept of a desiccant enhanced evaporative air conditioner (DEVap) with the objective of combining the benefits of liquid desiccant and evaporative cooling technologies into an innovative 'cooling core.' Liquid desiccant technologies have extraordinary dehumidification potential, but require an efficient cooling sink. DEVap's thermodynamic potential overcomes many shortcomings of standard refrigeration-based direct expansion cooling. DEVap decouples cooling and dehumidification performance, which results in independent temperature and humidity control. The energy input is largely switched away from electricity to low-grade thermal energy that can be sourced from fuels such as natural gas, waste heat, solar, or biofuels.

  3. Composite desiccant structure

    DOEpatents

    Fraioli, Anthony V.; Schertz, William W.

    1987-01-01

    A composite formed of small desiccant particles retained in a dark matrix composed of a porous binder containing a transition metal oxide with pores to provide moisture transport with respect to the particles, and metallic fibers to remove the heat of condensation during dehumidification and provide heat for the removal of moisture during regeneration. The moisture absorbing properties of the composite may be regenerated by exposure of the dark matrix to solar radiation with dehumidification occurring at night.

  4. Composite desiccant structure

    DOEpatents

    Fraioli, A.V.; Schertz, W.W.

    1984-06-06

    This patent discloses a composite formed of small desiccant particles retained in a dark matrix composed of a porous binder containing a transition metal oxide with pores to provide moisture transport with respect to the particles, and metallic fibers to remove the heat of condensation during dehumidification and provide heat for the removal of moisture during regeneration. The moisture absorbing properties of the composite may be regenerated by exposure of the dark matrix to solar radiation with dehumidification occurring at night.

  5. Low-Cost "Vacuum Desiccator"

    NASA Astrophysics Data System (ADS)

    Sweet, Frederick

    2004-10-01

    Described are individualized, low-cost, and safe desiccators that can be efficiently and rapidly made with an inexpensive kitchen aid sold for shrink-wrapping food. The device can be used for enclosing small vials or bottles and also jars that are too large to be placed in conventional glass or plastic desiccators. This shrink-wrapping device is proposed for producing "vacuum desiccators" in large undergraduate chemistry laboratories or in graduate and research laboratories.

  6. Influence of Ventilation Ratio on Desiccant Air Conditioning System's Efficiency Performance

    NASA Astrophysics Data System (ADS)

    Tran, Thien Nha; Akisawa, Atsushi; Kashiwagi, Takao; Hamamoto, Yoshinori

    Ventilation air is a concern for engineers since ventilated air controls indoor air contamination; additional ventilation, however, increases the energy consumption of buildings. The study investigates the energy efficiency performance of the desiccant dehumidification air conditioning system in the context of ventilation for a hot-humid climate such as summer in Japan. The investigation focuses on the variable ratio of ventilation air as required by the application of air conditioning system. The COP of the desiccant air conditioning system is determined. The evaluation is subsequently performed by comparing the desiccant based system with the conventional absorption cooling system and the vapor compression cooling system. Based on 12 desiccant rotor simulations, it is found that the desiccant regeneration temperature required varies between 47°C to 85°C as ventilation ratio increases from 0. 0 to 100%, and up to 52. 5°C as the ventilation ratio achieves 14%. The heat required for regenerating desiccant accounts for 55% and higher of the system's total heat consumption; the system is expected to be energy efficient by using wasted heat from the absorption chiller for desiccant regeneration; and its energy efficiency expands as the ratio of ventilation air rises above 15% compared with the conventional absorption cooling system. The energy efficiency also benefits as the ratio rises beyond 70% against the conventional vapor compression cooling system.

  7. Desiccant Dehumidification Wheel Test Guide

    SciTech Connect

    Slayzak, S.J.; Ryan, J.P.

    2001-01-25

    NREL's Desiccant Dehumidification Wheel Test Guide intends to facilitate execution of the new standards by certification labs and manufacturers. The Test Guide is a product of more than 20 years of experience gained at NREL's desiccant research facilities. It details practical experimental experience with rotary mass exchangers in relation to the standards and aims to develop testing expertise in industry quickly and cost effectively.

  8. Potential market analysis for residential solar assisted in-line heat pumps

    SciTech Connect

    Not Available

    1981-01-01

    The method of studying the performance of the solar-assisted heat pump using the FCHART 4.0 computer program is described. The solar-assisted heat pump's performance was compared to that of an air-to-air heat pump and found to be inferior. The lifetime energy requirement is expected to be greater, as is its life-cycle cost. Moreover, conventional heat pumps are available now and are more easily suited to retrofit applications. It is recommended that the solar-assisted heat pump program be terminated in favor of more identifiable significant residential energy programs. (LEW)

  9. Low-Flow Liquid Desiccant Air-Conditioning: Demonstrated Performance and Cost Implications

    SciTech Connect

    Kozubal, E.; Herrmann, L.; Deru, M.; Clark, J.; Lowenstein, A.

    2014-09-01

    Cooling loads must be dramatically reduced when designing net-zero energy buildings or other highly efficient facilities. Advances in this area have focused primarily on reducing a building's sensible cooling loads by improving the envelope, integrating properly sized daylighting systems, adding exterior solar shading devices, and reducing internal heat gains. As sensible loads decrease, however, latent loads remain relatively constant, and thus become a greater fraction of the overall cooling requirement in highly efficient building designs, particularly in humid climates. This shift toward latent cooling is a challenge for heating, ventilation, and air-conditioning (HVAC) systems. Traditional systems typically dehumidify by first overcooling air below the dew-point temperature and then reheating it to an appropriate supply temperature, which requires an excessive amount of energy. Another dehumidification strategy incorporates solid desiccant rotors that remove water from air more efficiently; however, these systems are large and increase fan energy consumption due to the increased airside pressure drop of solid desiccant rotors. A third dehumidification strategy involves high flow liquid desiccant systems. These systems require a high maintenance separator to protect the air distribution system from corrosive desiccant droplet carryover and so are more commonly used in industrial applications and rarely in commercial buildings. Both solid desiccant systems and most high-flow liquid desiccant systems (if not internally cooled) add sensible energy which must later be removed to the air stream during dehumidification, through the release of sensible heat during the sorption process.

  10. Review of open-cycle desiccant air-conditioning concepts and systems

    SciTech Connect

    Wurm, J.

    1986-08-01

    This paper attempts to overview the development status of desiccant cooling. Over the past 30 years of progressively intensifying attention, this promising technology has become a domain of interest of many research agencies and manufacturing companies. As a result, the market potential for machines based on desiccant processes, particularly in comfort cooling and agricultural applications, is getting close to realization. One of the most important incentives of developing heat-activated, open-cycle desiccant cooling machines (air conditioners) has always been its potential simplicity. Such premise has been deceiving to a degree that in many instances has slowed the progress. However, the persistent analytical and material research brought some desiccant systems close to the marketplace. They provide attractive alternatives to consumers and utilities, offering particularly effective humidity and temperature control in cases of high fresh-air-makeup requirements. The control of bacteria, airborne particulates, as well as CO/sub 2/, combined with effective heating capability make them attractive for controlled-atmosphere agriculture. Finally, the capability of using low-temperature waste heat to drive the cycle becomes an important attribute of a desiccant concept, specifically when combined with a regular vapor-compression cooling machine in energy saving space-conditioning concepts. The presented assessment concludes that, particularly for specialized applications, machines based on open-cycle desiccant cooling processes are very close to playing an important role in the space-conditioning (including comfort control) marketplace.

  11. Measurement uncertainty of adsorption testing of desiccant materials

    SciTech Connect

    Bingham, C E; Pesaran, A A

    1988-12-01

    The technique of measurement uncertainty analysis as described in the current ANSI/ASME standard is applied to the testing of desiccant materials in SERI`s Sorption Test Facility. This paper estimates the elemental precision and systematic errors in these tests and propagates them separately to obtain the resulting uncertainty of the test parameters, including relative humidity ({plus_minus}.03) and sorption capacity ({plus_minus}.002 g/g). Errors generated by instrument calibration, data acquisition, and data reduction are considered. Measurement parameters that would improve the uncertainty of the results are identified. Using the uncertainty in the moisture capacity of a desiccant, the design engineer can estimate the uncertainty in performance of a dehumidifier for desiccant cooling systems with confidence. 6 refs., 2 figs., 8 tabs.

  12. Desiccant outdoor air preconditioners maximize heat recovery ventilation potentials

    SciTech Connect

    Meckler, M.

    1995-12-31

    Microorganisms are well protected indoors by the moisture surrounding them if the relative humidity is above 70%. They can cause many acute diseases, infections, and allergies. Humidity also has an effect on air cleanliness and causes the building structure and its contents to deteriorate. Therefore, controlling humidity is a very important factor to human health and comfort and the structural longevity of a building. To date, a great deal of research has been done, and is continuing, in the use of both solid and liquid desiccants. This paper introduces a desiccant-assisted system that combines dehumidification and mechanical refrigeration by means of a desiccant preconditioning module that can serve two or more conventional air-conditioning units. It will be demonstrated that the proposed system, also having indirect evaporative cooling within the preconditioning module, can reduce energy consumption and provide significant cost savings, independent humidity and temperature control, and, therefore, improved indoor air quality and enhanced occupant comfort.

  13. Performance comparisons of naturally and mechanically ventilated solar-assisted nurseries

    SciTech Connect

    Milanuk, M.; Bodman, G.R.; DeShazer, J.A.; Schulte, D.

    1983-12-01

    When combined with solar floor heating and careful management, naturally ventilated swine nurseries can result in energy savings and comparable pig responses to mechanically ventilated nurseries of the current design that were also solar assisted.

  14. Development of marketable solar assisted heat pumps. Phase II. Summary report, technical results

    SciTech Connect

    Hundt, R.; Heard, S.

    1981-02-20

    A water source heat pump has been designed that is capable of operating over the range from 40 to 110/sup 0/F entering water temperature and has a heating coefficient of performance greater than six in the upper portion of this range. A computerized heat pump balance program was written to allow the performance of either a water-to-water or a water-to-air heat pump to be predicted in either the heating or cooling mode. A detailed program description, flow charts, and sample outputs are appended. The balance program was used to specify components for a high efficiency water-to-water and a high efficiency water-to-air heat pump. Performance predictions for both units in heating and in cooling are included. The water-to-water and water-to-air performance predictions were compared. The water-to-air approach was clearly superior. A detailed design and layout was done for the three solar-assisted water-to-air heat pump. A horizontal configuration was chosen for marketability reasons. The design was made consistent with high quantity production equipment available in our factories and should result in the lowest possible manufacturing cost. A key question to be answered in this project is whether a reciprocating compressor can operate without damage at the high suction pressures characteristic to the inline SAHP. Reciprocating compressors are normally applied in applications with maximum suction pressures equal to a 55/sup 0/F saturated suction temperature. An accelerated life test was performed on low speed at 85/sup 0/F saturated suction temperature and 100/sup 0/F saturated condensing temperature. After 465 hours at these severe conditions no performance degradation or physical damage was noted. Tests on high speed at 70/sup 0/F saturated suction temperature are in progress and should define the limits where the compressor may be safely operated.

  15. Review of Desiccant Dehumidification Technology

    SciTech Connect

    Pesaran, A. A.

    1994-10-01

    This paper overviews applications of desiccant technology for dehumidifying commercial and institutional buildings. Because of various market, policy, and regulatory factors, this technology is especially attractive for dehumidification applications in the I990s.

  16. Low-Flow Liquid Desiccant Air Conditioning: General Guidance and Site Considerations

    SciTech Connect

    Kozubal, E.; Herrmann, L.; Deru, M.; Clark, J.

    2014-09-01

    Dehumidification or latent cooling in buildings is an area of growing interest that has been identified as needing more research and improved technologies for higher performance. Heating, ventilating, and air-conditioning (HVAC) systems typically expend excessive energy by using overcool-and-reheat strategies to dehumidify buildings. These systems first overcool ventilation air to remove moisture and then reheat the air to meet comfort requirements. Another common strategy incorporates solid desiccant rotors that remove moisture from the air more efficiently; however, these systems increase fan energy consumption because of the high airside pressure drop of solid desiccant rotors and can add heat of absorption to the ventilation air. Alternatively, liquid desiccant air-conditioning (LDAC) technology provides an innovative dehumidification solution that: (1) eliminates the need for overcooling and reheating from traditional cooling systems; and (2) avoids the increased fan energy and air heating from solid desiccant rotor systems.

  17. Desiccant-Based Preconditioning Market Analysis

    SciTech Connect

    Fischer, J.

    2001-01-11

    A number of important conclusions can be drawn as a result of this broad, first-phase market evaluation. The more important conclusions include the following: (1) A very significant market opportunity will exist for specialized outdoor air-handling units (SOAHUs) as more construction and renovation projects are designed to incorporate the recommendations made by the ASHRAE 62-1989 standard. Based on this investigation, the total potential market is currently $725,000,000 annually (see Table 6, Sect. 3). Based on the market evaluations completed, it is estimated that approximately $398,000,000 (55%) of this total market could be served by DBC systems if they were made cost-effective through mass production. Approximately $306,000,000 (42%) of the total can be served by a non-regenerated, desiccant-based total recovery approach, based on the information provided by this investigation. Approximately $92,000,000 (13%) can be served by a regenerated desiccant-based cooling approach (see Table 7, Sect. 3). (2) A projection of the market selling price of various desiccant-based SOAHU systems was prepared using prices provided by Trane for central-station, air-handling modules currently manufactured. The wheel-component pricing was added to these components by SEMCO. This resulted in projected pricing for these systems that is significantly less than that currently offered by custom suppliers (see Table 4, Sect. 2). Estimated payback periods for all SOAHU approaches were quite short when compared with conventional over-cooling and reheat systems. Actual paybacks may vary significantly depending on site-specific considerations. (3) In comparing cost vs benefit of each SOAHU approach, it is critical that the total system design be evaluated. For example, the cost premium of a DBC system is very significant when compared to a conventional air handling system, yet the reduced chiller, boiler, cooling tower, and other expense often equals or exceeds this premium, resulting in a

  18. Implications of the lack of desiccation tolerance in recalcitrant seeds

    PubMed Central

    Berjak, Patricia; Pammenter, Norman W.

    2013-01-01

    A suite of interacting processes and mechanisms enables tolerance of desiccation and storage (conservation) of orthodox seeds in the dry state. While this is a long-term option under optimized conditions, dry orthodox seeds are not immortal, with life spans having been characterized as short, intermediate and long. Factors facilitating desiccation tolerance are metabolic “switch-off” and intracellular dedifferentiation. Recalcitrant seeds lack these mechanisms, contributing significantly to their desiccation sensitivity. Consequently, recalcitrant seeds, which are shed at high water contents, can be stored only in the short-term, under conditions not allowing dehydration. The periods of such hydrated storage are constrained by germination that occurs without the need for extraneous water, and the proliferation of seed-associated fungi. Cryopreservation is viewed as the only option for long-term conservation of the germplasm of recalcitrant-seeded species. This is not easily achieved, as each of the necessary procedures imposes oxidative damage. Intact recalcitrant seeds cannot be cryopreserved, the common practice being to use excised embryos or embryonic axes as explants. Dehydration is a necessary procedure prior to exposure to cryogenic temperatures, but this is associated with metabolism-linked injury mediated by uncontrolled reactive oxygen species generation and failing anti-oxidant systems. While the extent to which this occurs can be curtailed by maximizing drying rate (flash drying) it cannot be completely obviated. Explant cooling for, and rewarming after, cryostorage must necessarily be rapid, to avoid ice crystallization. The ramifications of desiccation sensitivity are discussed, as are problems involved in cryostorage, particularly those accompanying dehydration and damage consequent upon ice crystallization. While desiccation sensitivity is a “fact” of seed recalcitrance, resolutions of the difficulties involved germplasm conservation are

  19. Microbiological quality of desiccated coconut.

    PubMed Central

    Kinderlerer, J. L.; Clark, R. A.

    1986-01-01

    A microbial survey of Sri Lankan desiccated coconut has been made on material purchased in supermarkets in Sheffield or on material obtained directly from the processing company. The total viable count (TVC) was reduced by spoilage and pasteurization from 10(4)/g to 10(3)/g. Most samples contained low levels of coagulase-positive Staphylococcus aureus suggesting that this commodity had been handled during production. One focus of contamination with Aspergillus flavus was found for each 8.34 g of desiccated coconut (mean contamination). The number of bacteria and moulds in spoiled coconut was significantly lower than that in coconut obtained from the processor or purchased from retail outlets. It is suggested that the accumulation of free fatty acids, aliphatic methyl ketones and secondary alcohols produced during fungal spoilage has had a bactericidal and fungicidal effect. The use of microbial specifications for foods is questioned in situations where there is evidence of microbial spoilage having taken place. PMID:3081627

  20. Microbiological quality of desiccated coconut.

    PubMed

    Kinderlerer, J L; Clark, R A

    1986-02-01

    A microbial survey of Sri Lankan desiccated coconut has been made on material purchased in supermarkets in Sheffield or on material obtained directly from the processing company. The total viable count (TVC) was reduced by spoilage and pasteurization from 10(4)/g to 10(3)/g. Most samples contained low levels of coagulase-positive Staphylococcus aureus suggesting that this commodity had been handled during production. One focus of contamination with Aspergillus flavus was found for each 8.34 g of desiccated coconut (mean contamination). The number of bacteria and moulds in spoiled coconut was significantly lower than that in coconut obtained from the processor or purchased from retail outlets. It is suggested that the accumulation of free fatty acids, aliphatic methyl ketones and secondary alcohols produced during fungal spoilage has had a bactericidal and fungicidal effect. The use of microbial specifications for foods is questioned in situations where there is evidence of microbial spoilage having taken place.

  1. Development of Desiccant System using Wakkanai Siliceous Shale

    NASA Astrophysics Data System (ADS)

    Nakabayashi, Saya; Nagano, Katsunori; Nakamura, Makoto; Togawa, Junya; Kurokawa, Asami

    The aim of this study is to develop a desiccant system using Wakkanai siliceous shale. A honeycombed desiccant rotor containing this shale's powder and chlorides was made and evaluated. However a specific surface area and a pore volume were smaller than a silica-gel rotor or a zeolite rotor, the maximum amount of water adsorption was twice as other rotors. We have verified the function of this desiccant rotor concerning adsorption and desorption of moisture from the draft experiments. The rotor containing the shale could adsorb moisture stably in the cyclic test, and be regenerated by 40°C air under this experimental condition. This means that the exhaust heat from the heat pump can be used for regenerating rotor. Furthermore, the numerical simulation was carried out on the assumption that this rotor was used for a dehumidification for the residential air conditioning in Tokyo. This rotor could adsorb 37.1% moisture of the required dehumidification amount for the hottest day in 2008. When we employed a pre-cooling before dehumidification, the amount of adsorption increased to 66.2%.

  2. Active Desiccant Dehumidification Module Integration with Rooftop Packaged HVAC

    SciTech Connect

    Fischer, J

    2002-04-17

    This report summarizes a research and development program that produced a stand-alone active desiccant module (ADM) that can be easily integrated with new or existing packaged cooling equipment. The program also produced a fully integrated hybrid system, combining the active desiccant section with a conventional direct expansion air-conditioning unit, that resulted in a compact, low-cost, energy-efficient end product. Based upon the results of this investigation, both systems were determined to be highly viable products for commercialization. Major challenges--including wheel development, compact packaging, regeneration burner development, control optimization, and low-cost design--were all successfully addressed by the final prototypes produced and tested as part of this program. Extensive laboratory testing was completed in the SEMCO laboratory for each of the two ADM system approaches. This testing confirmed the performance of the ADM systems to be attractive compared with that of alternate approaches currently used to precondition outdoor air, where a return air path is not readily available for passive desiccant recovery or where first cost is the primary design criterion. Photographs, schematics, and performance maps are provided for the ADM systems that were developed; and many of the control advantages are discussed. Based upon the positive results of this research and development program, field tests are under way for fully instrumented pilot installations of ADM systems in both a hotel/motel and a restaurant.

  3. Cost analysis of new and retrofit hot-air type solar assisted heating systems

    NASA Technical Reports Server (NTRS)

    Stewart, R. D.; Hawkins, B. J.

    1978-01-01

    A detailed cost analysis/cost improvement study was performed on two Department of Energy/National Aeronautics and Space Administration operational test sites to determine actual costs and potential cost improvements of new and retrofit hot air type, solar assisted heating and hot water systems for single family sized structures. This analysis concentrated on the first cost of a system which included procurement, installation, and integration of a solar assisted heating and hot water system on a new or retrofit basis; it also provided several cost projections which can be used as inputs to payback analyses, depending upon the degree of optimism or future improvements assumed. Cost definitions were developed for five categories of cost, and preliminary estimates were developed for each. The costing methodology, approach, and results together with several candidate low cost designs are described.

  4. Thermal and economic analysis of solar-assisted heat pumps for low-temperature IPH applications

    NASA Astrophysics Data System (ADS)

    Chaturvedi, S. K.; Murphy, L. M.

    1981-10-01

    The potential technical and economic merits of solar assisted heat pump (SAHP) systems (which are high temperature when compared with residential applications) for low-temperature IPH applications are considered. The system looks more promising as the weather becomes more severe, and thus appears to have significant benefit in the less clement industrial areas of the country and in areas where land contraints are a concern.

  5. Monitoring Vadose Zone Desiccation with Geophysical Methods

    SciTech Connect

    Truex, Michael J.; Johnson, Timothy C.; Strickland, Christopher E.; Peterson, John E.; Hubbard, Susan S.

    2013-05-01

    Soil desiccation was recently field tested as a potential vadose zone remediation technology. Desiccation removes water from the vadose zone and significantly decreases the aqueous-phase permeability of the desiccated zone, thereby decreasing movement of moisture and contaminants. The 2-D and 3-D distribution of moisture content reduction over time provides valuable information for desiccation operations and for determining when treatment goals have been reached. This type of information can be obtained through use of geophysical methods. Neutron moisture logging, cross-hole electrical resistivity tomography, and cross-hole ground penetrating radar approaches were evaluated with respect to their ability to provide effective spatial and temporal monitoring of desiccation during a treatability study conducted in the vadose zone of the DOE Hanford Site in WA.

  6. Desiccation response of mammalian cells: anhydrosignaling.

    PubMed

    Huang, Zebo; Tunnacliffe, Alan

    2007-01-01

    Dehydration through evaporation, or air drying, is expected to have both similarities and differences to osmostress. Both stresses involve water loss, but the degree of dehydration will ultimately be more severe during desiccation. Despite the severity of desiccation stress, there are examples of organisms that can survive almost complete water loss, including resurrection plants and plant seeds, certain invertebrates among the nematodes, brine shrimps, tardigrades and bdelloid rotifers, and many microorganisms, including bakers' yeast. During desiccation, these organisms enter a state of suspended animation, a process known as anhydrobiosis ("life without water"). For other organisms, desiccation is lethal, but there is considerable interest in using what is known about anhydrobiosis to confer desiccation tolerance on sensitive cell types, such as mammalian cells. Success with this approach, which we have termed anhydrobiotic engineering, will require a more complete knowledge of the mechanisms of desiccation tolerance and the sensing and response of nontolerant organisms to extreme dehydration. With this goal in mind, we have attempted to characterize the response of human tissue culture cells to desiccation and to compare this response with osmotic upshift. This chapter describes some of the methods used to begin to uncover the response to evaporative water loss in human cell cultures.

  7. Performance predictions of silica-gel desiccant dehumidifiers. Technical report No. 3

    SciTech Connect

    Mathiprakasam, B.; Lavan, Z.

    1980-01-01

    The analysis of a cross-cooled desiccant dehumidifier using silica gel in the form of sheets is described. This unit is the principal component of solar powered desiccant air conditioning system. The mathematical model has first been formulated describing the dynamics of the dehumidifier. The model leads to a system of nonlinear coupled heat and mass transfer equations for the sorption processes and linear heat transfer equations for the purging processes. The model accounts for the gas film resistance and for the moisture diffusion in the desiccant. The governing equations are solved by a finite difference scheme to obtain periodic steady state solutions. The accuracy of the theoretical predictions is ascertained by comparing them with the experimental results. The performance of the dehumidifier, for a chosen set of initial conditions and dehumidifier parameters, has also been given.

  8. Water isotopes in desiccating lichens.

    PubMed

    Hartard, Britta; Cuntz, Matthias; Máguas, Cristina; Lakatos, Michael

    2009-12-01

    The stable isotopic composition of water is routinely used as a tracer to study water exchange processes in vascular plants and ecosystems. To date, no study has focussed on isotope processes in non-vascular, poikilohydric organisms such as lichens and bryophytes. To understand basic isotope exchange processes of non-vascular plants, thallus water isotopic composition was studied in various green-algal lichens exposed to desiccation. The study indicates that lichens equilibrate with the isotopic composition of surrounding water vapour. A model was developed as a proof of concept that accounts for the specific water relations of these poikilohydric organisms. The approach incorporates first their variable thallus water potential and second a compartmentation of the thallus water into two isotopically distinct but connected water pools. Moreover, the results represent first steps towards the development of poikilohydric organisms as a recorder of ambient vapour isotopic composition.

  9. Water isotopes in desiccating lichens

    PubMed Central

    Cuntz, Matthias; Máguas, Cristina; Lakatos, Michael

    2009-01-01

    The stable isotopic composition of water is routinely used as a tracer to study water exchange processes in vascular plants and ecosystems. To date, no study has focussed on isotope processes in non-vascular, poikilohydric organisms such as lichens and bryophytes. To understand basic isotope exchange processes of non-vascular plants, thallus water isotopic composition was studied in various green-algal lichens exposed to desiccation. The study indicates that lichens equilibrate with the isotopic composition of surrounding water vapour. A model was developed as a proof of concept that accounts for the specific water relations of these poikilohydric organisms. The approach incorporates first their variable thallus water potential and second a compartmentation of the thallus water into two isotopically distinct but connected water pools. Moreover, the results represent first steps towards the development of poikilohydric organisms as a recorder of ambient vapour isotopic composition. PMID:19888598

  10. [Desiccation cracking of soil body: a review].

    PubMed

    Pei, Yin-Ge; Xu, Ze-Min; Zhang, Jia-Ming

    2012-04-01

    Desiccation cracking of soil body is a complex physical process, which can affect the strength, stability, and permeability of soil body, and involve in several disciplines such as soil science, agricultural science, engineering geology, and environmental science. This paper introduced the significances of the study on the desiccation cracking of soil body, reviewed the related theoretical and applied researches and the quantitative analysis of crack morphology, and discussed the deficiencies in the research fields, research contents, and research methods. The future research directions about the desiccation cracking of soil body were pointed out.

  11. Parametric study of the cyclic behaviour of a hygroscopic matrix in a desiccant airflow system

    NASA Astrophysics Data System (ADS)

    Ruivo, C. R.; Costa, J. J.; Figueiredo, A. R.

    2011-09-01

    The study of the transport phenomena in desiccant airflow systems has been addressed in numerous research works, some of them concerning combined processes of cooling, dehumidification and energy recovery. In this paper a detailed numerical model is used to simulate the behaviour of a parallel-plate channel, cyclically exposed to two airflows with different inlet conditions, the plate being composed by a substrate and a desiccant porous layer. The modelled channel is considered to be representative of a real channel of a hygroscopic matrix that is operating at steady state regime, like it occurs in desiccant or enthalpy rotors. The numerical results are treated in order to represent the global behaviour of the hygroscopic rotor under steady state conditions. Results of a parametric study are presented as maps of isovalues of the heat and mass transfer rates and of the outlet states of both airflows, considering channels of distinct wall thickness, of different thickness of the desiccant and the subtract layers, together with wide ranges of the rotation speed and of the wheel partition. The mapped results presented provide an overview of the operation characteristics of hygroscopic rotors, allowing a quick determination of the optimum range of values for relevant parameters, such as the rotation speed and the wheel partition. The model is thus an interesting tool for design and manufacture purposes of enthalpy and desiccant wheels.

  12. Optimization of solar assisted heat pump systems via a simple analytic approach

    SciTech Connect

    Andrews, J W

    1980-01-01

    An analytic method for calculating the optimum operating temperature of the collector/storage subsystem in a solar assisted heat pump is presented. A tradeoff exists between rising heat pump coefficient of performance and falling collector efficiency as this temperature is increased, resulting in an optimum temperature whose value increases with increasing efficiency of the auxiliary energy source. Electric resistance is shown to be a poor backup to such systems. A number of options for thermally coupling the system to the ground are analyzed and compared.

  13. Junction formation during desiccation cracking.

    PubMed

    Toga, K B; Alaca, B Erdem

    2006-08-01

    In order to provide a sound physical basis for the understanding of the formation of desiccation crack networks, an experimental study is presented addressing junction formation. Focusing on junctions, basic features of the network determining the final pattern, provides an elemental approach and imparts conceptual clarity to the rather complicated problem of the evolution of crack patterns. Using coffee-water mixtures a clear distinction between junction formation during nucleation and propagation is achieved. It is shown that for the same drying suspension, one can switch from the well-known symmetric triple junctions that are unique to the nucleation phase to propagation junctions that are purely dictated by the variations of the stress state. In the latter case, one can even manipulate the path of a propagating crack in a deterministic fashion by changing the stress state within the suspension. Clear microscopic evidence is provided for the formation of propagation junctions, and material inhomogeneity is observed to be reflected by a broad distribution of angles, in stark contrast to shrinkage cracks in homogeneous solid films.

  14. Preliminary design package for residential heating/cooling system: Rankine air conditioner redesign

    NASA Technical Reports Server (NTRS)

    1978-01-01

    A summary of the preliminary redesign and development of a marketable single family heating and cooling system is presented. The interim design and schedule status of the residential (3-ton) redesign, problem areas and solutions, and the definition of plans for future design and development activities were discussed. The proposed system for a single-family residential heating and cooling system is a single-loop, solar-assisted, hydronic-to-warm-air heating subsystem with solar-assisted domestic water heating and a Rankine-driven expansion air-conditioning subsystem.

  15. Desiccation tolerance in Bryophytes: relevance to the evolution of desiccation tolerance in Land Plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The majority of desiccation-tolerant plants are found in the less complex clades that constitute the algae, lichens and bryophytes. However, within the larger and more complex groups of vascular land plants there are some 120-130 species that exhibit some degree of vegetative desiccation tolerance. ...

  16. Solar-Powered, Liquid-Desiccant Air Conditioner for Low-Electricity Humidity Control: Report and Summary Report

    SciTech Connect

    Dean, J.; Kozubal, E.; Herrmann, L.; Miller, J.; Lowenstein, A.; Barker, G.; Slayzak, S.

    2012-11-01

    The primary objective of this project was to demonstrate the capabilities of a new high-performance, liquid-desiccant dedicated outdoor air system (DOAS) to enhance cooling efficiency and comfort in humid climates while substantially reducing electric peak demand at Tyndall Air Force Base (AFB), which is 12 miles east of Panama City, Florida.

  17. Review of combined photovoltaic/thermal collector: solar assisted heat pump system options

    SciTech Connect

    Sheldon, D. B.; Russell, M. C.

    1980-01-01

    The advantages of using photovoltaic (PV) and combined photovoltaic/thermal (PV/T) collectors in conjunction with residential heat pumps are examined. The thermal and electrical power requirements of similar residences in New York City and Fort Worth are the loads under consideration. The TRNSYS energy balance program is used to simulate the operations of parallel, series, and cascade solar assisted heat pump systems. Similar work involving exclusively thermal collectors is reviewed, and the distinctions between thermal and PV/T systems are emphasized. Provided the defrost problem can be satisfactorily controlled, lifecycle cost analyses show that at both locations the optimum collector area is less than 50 m/sup 2/ and that the parallel system is preferred.

  18. Heat and mass transfer analysis of a desiccant dehumidifier matrix

    SciTech Connect

    Pesaran, A.A.

    1986-07-01

    This report documents the SERI Single-Blow Test Facility's design, fabrication, and testing for characterizing desiccant dehumidifiers for solar cooling applications. The first test article, a silica-gel parallel-plate dehumidifier with highly uniform passages, was designed and fabricated. Transient heat and mass transfer data and pressure drop data across the dehumidifier were obtained. Available heat and mass transfer models were extended to the parallel-place geometry, and the experimental data were compared with model predictions. Pressure drop measurements were also compared with model predictions of the fully developed laminar flow theory. The comparisons between the lumped-capacitance model and the experimental data were satisfactory. The pressure drop data compared satisfactorily with the theory (within 15%). A solid-side resistance model that is more detailed and does not assume symmetrical diffusion in particles was recommended for performance. This study has increased our understanding of the heat and mass transfer in silica gel parallel-plate dehumidifiers.

  19. Desiccation of unsaturated porous media: Intermediate-scale experiments and numerical simulation

    SciTech Connect

    Oostrom, Martinus; Wietsma, Thomas W.; Dane, J. H.; Truex, Michael J.; Ward, Anderson L.

    2009-08-01

    Soil desiccation (drying) is recognized as a potentially robust vadose zone remediation process involving water evaporation induced by air injection and extraction. Desiccation has the potential to immobilize contaminants and could potentially improve access for other gas-phase treatments by reducing water saturation and therefore increasing sediment gas-phase permeability. Before this technology could be deployed in the field, concerns related to energy limitations, osmotic effects, and potential contaminant remobilization after rewetting need to be addressed. A series of detailed wedge-shaped, intermediate-scale laboratory experiments in unsaturated homogeneous and simple heterogeneous systems was conducted to improve the understanding of the impact of energy balance issues on soil desiccation. The experiments were simulated with the multifluid flow simulator STOMP, using independently obtained hydraulic and thermal porous medium properties. In all the experiments, the injection of dry air proved to be an effective means for removing essentially all moisture from the test media. Evaporative cooling was observed which generally decreased with increased distance from the gas inlet chamber. Observations of temperature in fine-grained sands in the heterogeneous systems show two local temperature minima associated with the cooling. The first one occurs because of evaporation in the adjacent medium-grained sand whereas the second minimum is attributed to evaporative cooling in the fine-grained sand itself. Results of the laboratory tests were simulated accurately when thermal properties of the flow cell walls and insulation material were taken into account, indicating that the proper physics were incorporated into the simulator.

  20. Vapor pressures of the aqueous desiccants

    SciTech Connect

    Chung, T.W.; Luo, C.M.

    1999-09-01

    The vapor pressures of the aqueous desiccants lithium chloride, lithium bromide, calcium chloride, ethylene glycol, propylene glycol, and their mixtures were measured at their typical operating concentrations and at temperatures from 298 K to 313 K. The experimental data were fitted to an Antoine type of equation, ln[P(kPa)] = A {minus} B/[T(K) + C], where A, B, and C are constants and are concentration dependent. Vapor pressure data were further used to predict the effectiveness of dehumidification in liquid desiccant dehumidifiers.

  1. Experimental performance study of a proposed desiccant based air conditioning system.

    PubMed

    Bassuoni, M M

    2014-01-01

    An experimental investigation on the performance of a proposed hybrid desiccant based air conditioning system referred as HDBAC is introduced in this paper. HDBAC is mainly consisted of a liquid desiccant dehumidification unit integrated with a vapor compression system (VCS). The VCS unit has a cooling capacity of 5.27 kW and uses 134a as refrigerant. Calcium chloride (CaCl2) solution is used as the working desiccant material. HDBAC system is used to serve low sensible heat factor applications. The effect of different parameters such as, process air flow rate, desiccant solution flow rate, evaporator box and condenser box solution temperatures, strong solution concentration and regeneration temperature on the performance of the system is studied. The performance of the system is evaluated using some parameters such as: the coefficient of performance (COPa), specific moisture removal and energy saving percentage. A remarkable increase of about 54% in the coefficient of performance of the proposed system over VCS with reheat is achieved. A maximum overall energy saving of about 46% is observed which emphasizes the use of the proposed system as an energy efficient air conditioning system. PMID:25685475

  2. Experimental performance study of a proposed desiccant based air conditioning system.

    PubMed

    Bassuoni, M M

    2014-01-01

    An experimental investigation on the performance of a proposed hybrid desiccant based air conditioning system referred as HDBAC is introduced in this paper. HDBAC is mainly consisted of a liquid desiccant dehumidification unit integrated with a vapor compression system (VCS). The VCS unit has a cooling capacity of 5.27 kW and uses 134a as refrigerant. Calcium chloride (CaCl2) solution is used as the working desiccant material. HDBAC system is used to serve low sensible heat factor applications. The effect of different parameters such as, process air flow rate, desiccant solution flow rate, evaporator box and condenser box solution temperatures, strong solution concentration and regeneration temperature on the performance of the system is studied. The performance of the system is evaluated using some parameters such as: the coefficient of performance (COPa), specific moisture removal and energy saving percentage. A remarkable increase of about 54% in the coefficient of performance of the proposed system over VCS with reheat is achieved. A maximum overall energy saving of about 46% is observed which emphasizes the use of the proposed system as an energy efficient air conditioning system.

  3. Adsorption / Desorption Behavior of Water Vapor in an Adsorbent Desiccant Rotor

    NASA Astrophysics Data System (ADS)

    Tsujiguchi, Takuya; Kodama, Akio

    Adsorption / desorption behavior of water vapor onto desiccant rotor has been investigated to improve the desiccant cooling system by means of computer simulation. In this paper, we paid attention to the relationship between the equilibrium amount of water adsorbed onto the desiccant material and the relative humidity, that is adsorption isotherm as a principal characteristic feature of adsorbent. Considering actual adsorbents, five types of adsorption isotherms were assumed to clarify the influence of adsorption isotherm on the dehumidifying performance. After the investigation on the influences of some operating conditions on the dehumidifying performance at each selected adsorption isotherm, it was found that higher dehumidifying performance and reduction of length of desiccant rotor could be achieved by selecting appropriate adsorption isotherm. It was also predicted that S-shaped adsorption isotherm which is raised sharply at relative humidity around 15 % could produce the lowest air humidity at regeneration air temperature 80 °C. Moreover influence of the intraparticle diffusion coefficient which significantly influence on the adsorption / desorption rate was discussed choosing two adsorption isotherm from the above five isotherms. It seems that effective range of the intraparticle diffusion coefficient for the significant improvement of the dehumidifying performance was strongly influenced by the shape of adsorption isotherm.

  4. Adsorption/Desorption Behavior of Water Vapor in an Adsorbent Desiccant Rotor

    NASA Astrophysics Data System (ADS)

    Tsujiguchi, Takuya; Kodama, Akio

    To clarify the operating and design concept of desiccant rotor, which is a most important component of an adsorptive desiccant cooling process, adsorption / desorption behavior of water vapor in a desiccant rotor has been investigated by means of computer simulation. Mass transfer coefficient in the mathematical model could be related to cycle time by applying the penetration theory. Considering this relationship, influences of the rotation speed of the desiccant rotor, process / regeneration air velocity and their velocity ratio were investigated. It was found that the optimum rotation speed tended to disappear when the regeneration air temperature was low and its humidity was considerably small compared to the process inlet air, since the product air condition approached to regeneration air condition as the rotation speed increased. Decrease of the dehumidifying performance was observed at higher air velocity and the corresponding higher rotation speed since the adsorbent rotor was not fully regenerated due to shorter regeneration time and shorter residence time of process / regeneration air in the adsorbent rotor prevented the mass transfer between air and adsorbent. It was also found that the dehumidifying performance was not improved even though the adsorbent was fully regenerated by higher regeneration air velocity as the sensible heat transferred from the regeneration zone via adsorbent itself increased and disturbed adsorption.

  5. C4 grasses prosper as carbon dioxide eliminates desiccation in warmed semi-arid grassland.

    PubMed

    Morgan, Jack A; LeCain, Daniel R; Pendall, Elise; Blumenthal, Dana M; Kimball, Bruce A; Carrillo, Yolima; Williams, David G; Heisler-White, Jana; Dijkstra, Feike A; West, Mark

    2011-08-03

    Global warming is predicted to induce desiccation in many world regions through increases in evaporative demand. Rising CO(2) may counter that trend by improving plant water-use efficiency. However, it is not clear how important this CO(2)-enhanced water use efficiency might be in offsetting warming-induced desiccation because higher CO(2) also leads to higher plant biomass, and therefore greater transpirational surface. Furthermore, although warming is predicted to favour warm-season, C(4) grasses, rising CO(2) should favour C(3), or cool-season plants. Here we show in a semi-arid grassland that elevated CO(2) can completely reverse the desiccating effects of moderate warming. Although enrichment of air to 600 p.p.m.v. CO(2) increased soil water content (SWC), 1.5/3.0 °C day/night warming resulted in desiccation, such that combined CO(2) enrichment and warming had no effect on SWC relative to control plots. As predicted, elevated CO(2) favoured C(3) grasses and enhanced stand productivity, whereas warming favoured C(4) grasses. Combined warming and CO(2) enrichment stimulated above-ground growth of C(4) grasses in 2 of 3 years when soil moisture most limited plant productivity. The results indicate that in a warmer, CO(2)-enriched world, both SWC and productivity in semi-arid grasslands may be higher than previously expected.

  6. Experimental performance study of a proposed desiccant based air conditioning system

    PubMed Central

    Bassuoni, M.M.

    2013-01-01

    An experimental investigation on the performance of a proposed hybrid desiccant based air conditioning system referred as HDBAC is introduced in this paper. HDBAC is mainly consisted of a liquid desiccant dehumidification unit integrated with a vapor compression system (VCS). The VCS unit has a cooling capacity of 5.27 kW and uses 134a as refrigerant. Calcium chloride (CaCl2) solution is used as the working desiccant material. HDBAC system is used to serve low sensible heat factor applications. The effect of different parameters such as, process air flow rate, desiccant solution flow rate, evaporator box and condenser box solution temperatures, strong solution concentration and regeneration temperature on the performance of the system is studied. The performance of the system is evaluated using some parameters such as: the coefficient of performance (COPa), specific moisture removal and energy saving percentage. A remarkable increase of about 54% in the coefficient of performance of the proposed system over VCS with reheat is achieved. A maximum overall energy saving of about 46% is observed which emphasizes the use of the proposed system as an energy efficient air conditioning system. PMID:25685475

  7. Analytical and experimental evaluation of solar absorption and vapor compression residential cooling systems

    SciTech Connect

    Darkazalli, G.; Lawley, T.J.

    1981-01-01

    Results from the University of Texas at Arlington/Solar Energy Research Facility (UTA/SERF) analytical and experimental study on residential solar heating and cooling systems are given. A description of the 145 m/sup 2/ solar residence, its basic components, operation, and controls of various combinations of system components is presented. A comparison of solar powered absorption cooling and solar assisted electric powered heat pump cooling for a single family residence is also presented. 2 refs.

  8. Comparative metabolic profiling between desiccation-sensitive and desiccation-tolerant species of Selaginella reveals insights into the resurrection trait

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Spike-mosses (Selaginellaceae) represent an ancient lineage of vascular plants in which some species have evolved or revolved desiccation tolerance (DT). A sister group comparison was conducted between a desiccation-tolerant species, Selaginella lepidophylla, and a desiccation-sensitive species, S. ...

  9. Desiccation tolerance in bryophytes: a review

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Desiccation tolerance, the ability to lose virtually all free intracellular water and then recover normal function upon rehydration, is one of the most remarkable features of bryophytes. The physiology of bryophytes differs in major respects from that of vascular plants by virtue of their smaller s...

  10. Trehalose Accumulation Triggers Autophagy during Plant Desiccation

    PubMed Central

    Moghaddam, Lalehvash; Long, Hao; Dickman, Martin B; Zhang, Xiuren; Mundree, Sagadevan

    2015-01-01

    Global climate change, increasingly erratic weather and a burgeoning global population are significant threats to the sustainability of future crop production. There is an urgent need for the development of robust measures that enable crops to withstand the uncertainty of climate change whilst still producing maximum yields. Resurrection plants possess the unique ability to withstand desiccation for prolonged periods, can be restored upon watering and represent great potential for the development of stress tolerant crops. Here, we describe the remarkable stress characteristics of Tripogon loliiformis, an uncharacterised resurrection grass and close relative of the economically important cereals, rice, sorghum, and maize. We show that T. loliiformis survives extreme environmental stress by implementing autophagy to prevent Programmed Cell Death. Notably, we identified a novel role for trehalose in the regulation of autophagy in T.loliiformis. Transcriptome, Gas Chromatography Mass Spectrometry, immunoblotting and confocal microscopy analyses directly linked the accumulation of trehalose with the onset of autophagy in dehydrating and desiccated T. loliiformis shoots. These results were supported in vitro with the observation of autophagosomes in trehalose treated T. loliiformis leaves; autophagosomes were not detected in untreated samples. Presumably, once induced, autophagy promotes desiccation tolerance in T.loliiformis, by removal of cellular toxins to suppress programmed cell death and the recycling of nutrients to delay the onset of senescence. These findings illustrate how resurrection plants manipulate sugar metabolism to promote desiccation tolerance and may provide candidate genes that are potentially useful for the development of stress tolerant crops. PMID:26633550

  11. Performance analysis of solar-assisted chemical heat-pump dryer

    SciTech Connect

    Fadhel, M.I.; Sopian, K.; Daud, W.R.W.

    2010-11-15

    A solar-assisted chemical heat-pump dryer has been designed, fabricated and tested. The performance of the system has been studied under the meteorological conditions of Malaysia. The system consists of four main components: solar collector (evacuated tubes type), storage tank, solid-gas chemical heat pump unit and dryer chamber. A solid-gas chemical heat pump unit consists of reactor, condenser and evaporator. The reaction used in this study (CaCl2-NH{sub 3}). A simulation has been developed, and the predicted results are compared with those obtained from experiments. The maximum efficiency for evacuated tubes solar collector of 80% has been predicted against the maximum experiment of 74%. The maximum values of solar fraction from the simulation and experiment are 0.795 and 0.713, respectively, whereas the coefficient of performance of chemical heat pump (COP{sup h}) maximum values 2.2 and 2 are obtained from simulation and experiments, respectively. The results show that any reduction of energy at condenser as a result of the decrease in solar radiation will decrease the coefficient of performance of chemical heat pump as well as decrease the efficiency of drying. (author)

  12. Development of a Solar Assisted Drying System Using Double-Pass Solar Collector with Finned Absorber

    NASA Astrophysics Data System (ADS)

    Azmi, M. S. M.; Othman, M. Y.; Sopian, K.; Ruslan, M. H.; Majid, Z. A. A.; Fudholi, A.; Yasin, J. M.

    2012-09-01

    The Solar Energy Research Group, Universiti Kebangsaan Malaysia, International Islamic University Malaysia and Yayasan FELDA has designed and constructed a solar assisted drying system at OPF FELDA Factory, Felda Bukit Sagu 2, Kuantan, Pahang. The drying system has a total of six double-pass solar collectors. Each collector has a length of 480 cm and a width of 120 cm. The first channel depth is 3.5 cm and the second channel depth is 7 cm. Longitudinal fins made of angle aluminium, 0.8 mm thickness were attached to the bottom surface of the absorber plate. The solar collectors are arranged as two banks of three collectors each in series. Internal manifold are used to connect the collectors. Air enters through the first channel and then through the second channel of the collector. An auxiliary heater source is installed to supply heat under unfavourable solar radiation condition. An on/off controller is used to control the startup and shutdown of the auxiliary heater. An outlet temperature of 70-75 °C can be achieved at solar radiation range of 800-900 W/m2 and flow rate of 0.12 kg/s. The average thermal efficiency of a solar collector is approximately 37%.

  13. Solar-assisted gas-energy water-heating feasibility for apartments

    NASA Technical Reports Server (NTRS)

    Davis, E. S.

    1975-01-01

    Studies of residential energy use, solar-energy technology for buildings, and the requirements for implementing technology in the housing industry led to a project to develop a solar water heater for apartments. A design study for a specific apartment was used to establish a solar water-heater cost model which is based on plumbing contractor bids and manufacturer estimates. The cost model was used to size the system to minimize the annualized cost of hot water. The annualized cost of solar-assisted gas-energy water heating is found to be less expensive than electric water heating but more expensive than gas water heating. The feasibility of a natural gas utility supplying the auxiliary fuel is evaluated. It is estimated that gas-utilizing companies will find it profitable to offer solar water heating as part of a total energy service option or on a lease basis when the price of new base-load supplies of natural gas reaches $2.50-$3.00 per million Btu.

  14. Indirect evaporative cooler using membrane-contained, liquid desiccant for dehumidification

    SciTech Connect

    Kozubal, Eric Joseph; Slayzak, Steven Joseph

    2014-07-08

    An indirect evaporative cooler for cooling inlet supply air from a first temperature to a second, lower temperature using a stream of liquid coolant and a stream of exhaust or purge air. The cooler includes a first flow channel for inlet supply air and a second flow channel adjacent the first for exhaust air. The first and second flow channels are defined in part by sheets of a membrane permeable to water vapor such that mass is transferred as a vapor through the membrane from the inlet supply air to a contained liquid desiccant for dehumidification and also to the exhaust air as heat is transferred from the inlet supply air to the liquid coolant. A separation wall divides the liquid desiccant and the coolant but allows heat to be transferred from the supply air to the coolant which releases water vapor to the counter or cross flowing exhaust air.

  15. Liquid-Desiccant Vapor Separation Reduces the Energy Requirements of Atmospheric Moisture Harvesting.

    PubMed

    Gido, Ben; Friedler, Eran; Broday, David M

    2016-08-01

    An innovative atmospheric moisture harvesting system is proposed, where water vapor is separated from the air prior to cooling and condensation. The system was studied using a model that simulates its three interconnected cycles (air, desiccant, and water) over a range of ambient conditions, and optimal configurations are reported for different operation conditions. Model results were compared to specifications of commercial atmospheric moisture harvesting systems and found to represent saving of 5-65% of the electrical energy requirements due to the vapor separation process. We show that the liquid desiccant separation stage that is integrated into atmospheric moisture harvesting systems can work under a wide range of environmental conditions using low grade or solar heating as a supplementary energy source, and that the performance of the combined system is superior.

  16. Frost Growth CFD Model of an Integrated Active Desiccant Rooftop Unit

    SciTech Connect

    Geoghegan, Patrick J; Petrov, Andrei Y; Vineyard, Edward Allan; Zaltash, Abdolreza; Linkous, Randall Lee

    2008-01-01

    A frost growth model is incorporated into a Computational Fluid Dynamics (CFD) simulation of a heat pump by means of a user-defined function in FLUENT, a commercial CFD code. The transient model is applied to the outdoor section of an Integrated Active Desiccant Rooftop (IADR) unit in heating mode. IADR is a hybrid vapor compression and active desiccant unit capable of handling 100% outdoor air (dedicated outdoor air system) or as a total conditioning system, handling both outdoor air and space cooling or heating loads. The predicted increase in flow resistance and loss in heat transfer capacity due to frost build-up are compared to experimental pressure drop readings and thermal imaging. The purpose of this work is to develop a CFD model that is capable of predicting frost growth, an invaluable tool in evaluating the effectiveness of defrost-on-demand cycles.

  17. Liquid-Desiccant Vapor Separation Reduces the Energy Requirements of Atmospheric Moisture Harvesting.

    PubMed

    Gido, Ben; Friedler, Eran; Broday, David M

    2016-08-01

    An innovative atmospheric moisture harvesting system is proposed, where water vapor is separated from the air prior to cooling and condensation. The system was studied using a model that simulates its three interconnected cycles (air, desiccant, and water) over a range of ambient conditions, and optimal configurations are reported for different operation conditions. Model results were compared to specifications of commercial atmospheric moisture harvesting systems and found to represent saving of 5-65% of the electrical energy requirements due to the vapor separation process. We show that the liquid desiccant separation stage that is integrated into atmospheric moisture harvesting systems can work under a wide range of environmental conditions using low grade or solar heating as a supplementary energy source, and that the performance of the combined system is superior. PMID:27435379

  18. Desiccant-Based Combined Systems: Integrated Active Desiccant Rooftop Hybrid System Development and Testing Final Report- Phase 4

    SciTech Connect

    Fischer, J

    2005-05-06

    provide individual sensible and latent loads required by an occupied space without over-cooling and reheating air. The product was developed using a housing construction similar to that of a conventional packaged rooftop unit. The resulting integrated active desiccant rooftop (IADR) is similar in size to a currently available conventional rooftop unit sized to provide an equivalent total cooling capacity. Unlike a conventional rooftop unit, the IADR can be operated as a dedicated outdoor air system processing 100% outdoor air, as well as a total conditioning system capable of handling any ratio of return air to outdoor air. As part of this R&D program, a detailed investigation compared the first cost and operating cost of the IADR with costs for a conventional packaged approach for an office building located in Jefferson City, MO. The results of this comparison suggest that the IADR approach, once commercialized, could be cost-competitive with existing technology--exhibiting a one-year to two-year payback period--while simultaneously offering improved humidity control, indoor air quality, and energy efficiency.

  19. Field Demonstration of Active Desiccant-Based Outdoor Air Preconditioning Systems, Final Report: Phase 3

    SciTech Connect

    Fischer, J.

    2001-07-09

    This report summarizes an investigation of the performance of two active desiccant cooling systems that were installed as pilot systems in two locations--a college dormitory and a research laboratory--during the fall of 1999. The laboratory system was assembled in the field from commercially available Trane air-handling modules combined with a standard total energy recovery module and a customized active desiccant wheel, both produced by SEMCO. The dormitory system was a factory-built, integrated system produced by SEMCO that included both active desiccant and sensible-only recovery wheels, a direct-fired gas regeneration section, and a pre-piped Trane heat pump condensing section. Both systems were equipped with direct digital control systems, complete with full instrumentation and remote monitoring capabilities. This report includes detailed descriptions of these two systems, installation details, samples of actual performance, and estimations of the energy savings realized. These pilot sites represent a continuation of previous active desiccant product development research (Fischer, Hallstrom, and Sand 2000; Fischer 2000). Both systems performed as anticipated, were reliable, and required minimal maintenance. The dehumidification/total-energy-recovery hybrid approach was particularly effective in all respects. System performance showed remarkable improvement in latent load handling capability and operating efficiency compared with the original conventional cooling system and with the conventional system that remained in another, identical wing of the facility. The dehumidification capacity of the pilot systems was very high, the cost of operation was very low, and the system was cost-effective, offering a simple payback for these retrofit installations of approximately 5 to 6 years. Most important, the dormitory system resolved numerous indoor air quality problems in the dormitory by providing effective humidity control and increased, continuous ventilation air.

  20. TOR and RAS pathways regulate desiccation tolerance in Saccharomyces cerevisiae

    PubMed Central

    Welch, Aaron Z.; Gibney, Patrick A.; Botstein, David; Koshland, Douglas E.

    2013-01-01

    Tolerance to desiccation in cultures of Saccharomyces cerevisiae is inducible; only one in a million cells from an exponential culture survive desiccation compared with one in five cells in stationary phase. Here we exploit the desiccation sensitivity of exponentially dividing cells to understand the stresses imposed by desiccation and their stress response pathways. We found that induction of desiccation tolerance is cell autonomous and that there is an inverse correlation between desiccation tolerance and growth rate in glucose-, ammonia-, or phosphate-limited continuous cultures. A transient heat shock induces a 5000–fold increase in desiccation tolerance, whereas hyper-ionic, -reductive, -oxidative, or -osmotic stress induced much less. Furthermore, we provide evidence that the Sch9p-regulated branch of the TOR and Ras-cAMP pathway inhibits desiccation tolerance by inhibiting the stress response transcription factors Gis1p, Msn2p, and Msn4p and by activating Sfp1p, a ribosome biogenesis transcription factor. Among 41 mutants defective in ribosome biogenesis, a subset defective in 60S showed a dramatic increase in desiccation tolerance independent of growth rate. We suggest that reduction of a specific intermediate in 60S biogenesis, resulting from conditions such as heat shock and nutrient deprivation, increases desiccation tolerance. PMID:23171550

  1. Desiccant-based dehumidification system and method

    DOEpatents

    Fischer, John C.

    2004-06-22

    The present invention provides an apparatus for dehumidifying air supplied to an enclosed space by an air conditioning unit. The apparatus includes a partition separating the interior of the housing into a supply portion and a regeneration portion. The supply portion has an inlet for receiving supply air from the air conditioning unit and an outlet for supplying air to the enclosed space. A regeneration fan creates the regeneration air stream. The apparatus includes an active desiccant wheel positioned such that a portion of the wheel extends into the supply portion and a portion of the wheel extends into the regeneration portion, so that the wheel can rotate through the supply air stream and the regeneration air stream to dehumidify the supply air stream. A heater warms the regeneration air stream as necessary to regenerate the desiccant wheel. The invention also comprises a hybrid system that combines air conditioning and dehumidifying components into a single integrated unit.

  2. A review of desiccant dehumidification technology

    SciTech Connect

    Pesaran, A.A.

    1994-10-01

    This paper overviews applications of desiccant technology for dehumidifying commercial and institutional buildings. Because of various market, policy, and regulatory factors, this technology is especially attractive for dehumidification applications in the 1990s. After briefly reviewing the principle of operation, the authors present three case studies-for supermarkets, a hotel, and an office building. The authors also discuss recent advances and ongoing research and development activities.

  3. Energy-efficient regenerative liquid desiccant drying process

    DOEpatents

    Ko, Suk M.; Grodzka, Philomena G.; McCormick, Paul O.

    1980-01-01

    This invention relates to the use of desiccants in conjunction with an open oop drying cycle and a closed loop drying cycle to reclaim the energy expended in vaporizing moisture in harvested crops. In the closed loop cycle, the drying air is brought into contact with a desiccant after it exits the crop drying bin. Water vapor in the moist air is absorbed by the desiccant, thus reducing the relative humidity of the air. The air is then heated by the used desiccant and returned to the crop bin. During the open loop drying cycle the used desiccant is heated (either fossil or solar energy heat sources may be used) and regenerated at high temperature, driving water vapor from the desiccant. This water vapor is condensed and used to preheat the dilute (wet) desiccant before heat is added from the external source (fossil or solar). The latent heat of vaporization of the moisture removed from the desiccant is reclaimed in this manner. The sensible heat of the regenerated desiccant is utilized in the open loop drying cycle. Also, closed cycle operation implies that no net energy is expended in heating drying air.

  4. Proteome analysis of leaves of the desiccation-tolerant grass, sporobolus stapfianus, in response to desiccation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sporobolus stapfianus is a resurrection grass native to South Africa which can tolerate the complete drying of its vegetative tissue structure; i.e., desiccation, and recover fully within hours of rewetting. Gene expression studies have demonstrated that the grass employs a strategy of gene inductio...

  5. A desiccant dehumidifier for electric vehicle heating

    SciTech Connect

    Aceves, S.M.; Smith, J.R.

    1996-09-01

    Vehicle heating requires a substantial amount of energy. Engines in conventional cars produce enough waste heat to provide comfort heating and defogging/defrosting, even under very extreme conditions. Electric vehicles (EVs), however, generate little waste heat. Using battery energy for heating may consume a substantial fraction of the energy storage capacity, reducing the vehicle range, which is one of the most important parameters in determining EV acceptability. Water vapor generated by the vehicle passengers is in large part responsible for the high heating loads existing in vehicles. In cold climates, the generation of water vapor inside the car may result in water condensation on the windows, diminishing visibility. Two strategies are commonly used to avoid condensation on windows: windows are kept warm, and a large amount of ambient air is introduced in the vehicle. Either strategy results in a substantial heating load. These strategies are often used in combination, and a trade-off exists between them. If window temperature is decreased, ventilation rate has to be increased. Reducing the ventilation rate requires an increase of the temperature of the windows to prevent condensation. An alternative solution is a desiccant dehumidifier, which adsorbs water vapor generated by the passengers. Window temperatures and ventilation rates can then be reduced, resulting in a substantially lower heating load. This paper explores the dehumidifier heating concept. The first part shows the energy savings that could be obtained by using this technology. The second part specifies the required characteristics and dimensions of the system. The results indicate that the desiccant system can reduce the steady-state heating load by 60% or more under typical conditions. The reduction in heating load is such that waste heat may be enough to provide the required heating under most ambient conditions. Desiccant system dimensions and weight appear reasonable for packaging in an EV.

  6. Molecular mechanisms of desiccation tolerance in resurrection plants.

    PubMed

    Gechev, Tsanko S; Dinakar, Challabathula; Benina, Maria; Toneva, Valentina; Bartels, Dorothea

    2012-10-01

    Resurrection plants are a small but diverse group of land plants characterized by their tolerance to extreme drought or desiccation. They have the unique ability to survive months to years without water, lose most of the free water in their vegetative tissues, fall into anabiosis, and, upon rewatering, quickly regain normal activity. Thus, they are fundamentally different from other drought-surviving plants such as succulents or ephemerals, which cope with drought by maintaining higher steady state water potential or via a short life cycle, respectively. This review describes the unique physiological and molecular adaptations of resurrection plants enabling them to withstand long periods of desiccation. The recent transcriptome analysis of Craterostigma plantagineum and Haberlea rhodopensis under drought, desiccation, and subsequent rehydration revealed common genetic pathways with other desiccation-tolerant species as well as unique genes that might contribute to the outstanding desiccation tolerance of the two resurrection species. While some of the molecular responses appear to be common for both drought stress and desiccation, resurrection plants also possess genes that are highly induced or repressed during desiccation with no apparent sequence homologies to genes of other species. Thus, resurrection plants are potential sources for gene discovery. Further proteome and metabolome analyses of the resurrection plants contributed to a better understanding of molecular mechanisms that are involved in surviving severe water loss. Understanding the cellular mechanisms of desiccation tolerance in this unique group of plants may enable future molecular improvement of drought tolerance in crop plants.

  7. Potential of solar cooling systems for peak demand reduction

    SciTech Connect

    Pesaran, A A; Neymark, J

    1994-11-01

    We investigated the technical feasibility of solar cooling for peak demand reduction using a building energy simulation program (DOE2.1D). The system studied was an absorption cooling system with a thermal coefficient of performance of 0.8 driven by a solar collector system with an efficiency of 50% with no thermal storage. The analysis for three different climates showed that, on the day with peak cooling load, about 17% of the peak load could be met satisfactorily with the solar-assisted cooling system without any thermal storage. A performance availability analysis indicated that the solar cooling system should be designed for lower amounts of available solar resources that coincide with the hours during which peak demand reduction is required. The analysis indicated that in dry climates, direct-normal concentrating collectors work well for solar cooling; however, in humid climates, collectors that absorb diffuse radiation work better.

  8. Experimental study on performance of celdek packed liquid desiccant dehumidifier

    NASA Astrophysics Data System (ADS)

    Kumar, Rakesh; Asati, A. K.

    2016-09-01

    Dehumidifier is the main component of liquid desiccant dehumidification system. Effect of the inlet parameters on various outlet parameters of the dehumidifier is studied in the present paper with structured pads as packing material and calcium chloride as liquid desiccant to process the air. The outlet parameters are change in specific humidity, mass transfer coefficient, moisture removal rate, air temperature, solution temperature, effectiveness and the corresponding inlet process parameters; mass flow rate of air, temperature of air, temperature and flow rate of desiccant solution. It is observed that mass transfer coefficient and moisture removal rate increase with increasing mass flow rate of the air and desiccant while these parameters decrease with increasing temperature of air and desiccant solution. Dehumidifier effectiveness gets increased with increasing solution flow rate. The present investigations are compared with the results of the researchers in the past.

  9. Desiccation resistance in four Drosophila species: sex and population effects.

    PubMed

    Matzkin, Luciano M; Watts, Thomas D; Markow, Therese A

    2007-01-01

    Desiccation resistance and body mass were measured in multiple populations of each of four species of Drosophila: two desert endemic species (D. nigrospiracula and D. mojavensis), and two with more widespread distributions (D. melanogaster and D. pseudoobscura). While flies from the desert species were more desiccation tolerant, there was, in certain cases, significant variation in desiccation resistance among populations of the same species. A significant difference in desiccation resistance was observed between the sexes, females were more resistant than males, but this relationship was reversed when taking into account body mass differences between the sexes. The degree of observed within-species variability demonstrates that studies focusing upon differences between species can produce different conclusions if they rely on observations for only single populations of a given species. Our data also suggest the existence of multiple mechanisms for desiccation resistance. PMID:18836314

  10. Effect of supply/regeneration section area ratio on the performance of desiccant wheels in hot and humid climates: an experimental investigation

    NASA Astrophysics Data System (ADS)

    Zendehboudi, Alireza; Esmaeili, Hossein

    2016-06-01

    Desiccant cooling system is a suitable alternative option for conventional cooling system in humid climates. It is an environmental protection technique for cooling buildings. This study has investigated the effect of supply/regeneration section area ratio on the performance of desiccant wheels in hot and humid climates, using Silica Gel (WSG) and Molecular Sieve (LT3) desiccants. To this end, some parameters such as outlet air humidity ratio, process removed moisture, process outlet temperature, reactivation outlet temperature and reactivation outlet moisture have been examined as a function of rotational speed and inlet air humidity ratio in 1:3, 1:2 and 1:1 split. In this study, desiccant materials are regenerated using a constant regeneration temperature of 80 °C, wheel rotation speed range of 4-12 RPH (revolutions per hour) and variable humidity. The results show that a rise in area ratio causes an increase in process removed moisture, process outlet temperature, reactivation outlet temperature and a drop in reactivation outlet moisture and outlet humidity ratio of process air.

  11. Desiccation stress induces developmental heterochrony in Drosophila melanogaster.

    PubMed

    Thorat, Leena; Oulkar, Dasharath P; Banerjee, Kaushik; Nath, Bimalendu B

    2016-09-01

    Stressful environments are known to perturb developmental patterns in insects. In the purview of desiccation as a stressor, relatively little is known about the developmental consequences linked with desiccation tolerance. In this study, we have particularly focused on the exploration of the temporal profile of postembryonic development in response to desiccation exposure in Drosophila melanogaster and the associated trade-offs. We document a correlation between variations in 20-hydroxyecdysone levels and the altered timing of metamorphic events during the life cycle. Following desiccation, we observed an extension in the larval longevity whereas the duration of the pupal and adult stages was significantly shortened. Alternately, feeding of 20-hydroxyecdysone apparently led to the restoration of the normal temporal pattern of development in the desiccated group. In spite of the desiccation-responsive heterochronic shifts in development, the overall lifespan post recovery remained almost unaltered among the desiccated and undesiccated groups suggesting plasticity in developmental control. This observation reminisces 'canalization-like' phenomenon that buffers alterations in the overall lifespan. We thus identified a desiccationresponsive period in the lifespan of D. melanogaster during which variations in ecdysone levels are capable to alter the temporal course of development. PMID:27581925

  12. Desiccation stress induces developmental heterochrony in Drosophila melanogaster.

    PubMed

    Thorat, Leena; Oulkar, Dasharath P; Banerjee, Kaushik; Nath, Bimalendu B

    2016-09-01

    Stressful environments are known to perturb developmental patterns in insects. In the purview of desiccation as a stressor, relatively little is known about the developmental consequences linked with desiccation tolerance. In this study, we have particularly focused on the exploration of the temporal profile of postembryonic development in response to desiccation exposure in Drosophila melanogaster and the associated trade-offs. We document a correlation between variations in 20-hydroxyecdysone levels and the altered timing of metamorphic events during the life cycle. Following desiccation, we observed an extension in the larval longevity whereas the duration of the pupal and adult stages was significantly shortened. Alternately, feeding of 20-hydroxyecdysone apparently led to the restoration of the normal temporal pattern of development in the desiccated group. In spite of the desiccation-responsive heterochronic shifts in development, the overall lifespan post recovery remained almost unaltered among the desiccated and undesiccated groups suggesting plasticity in developmental control. This observation reminisces 'canalization-like' phenomenon that buffers alterations in the overall lifespan. We thus identified a desiccationresponsive period in the lifespan of D. melanogaster during which variations in ecdysone levels are capable to alter the temporal course of development.

  13. Thermodynamic performance of a hybrid air cycle refrigeration system using a desiccant rotor

    NASA Astrophysics Data System (ADS)

    Hwang, Kyudae; Song, Chan Ho; Kim, Sung Ki; Saito, Kiyoshi; Kawai, Sunao

    2013-03-01

    Due to the concern on global warming, the demand for a system using natural refrigerant is increasing and many researches have been devoted to develop systems with natural refrigerants. Among natural refrigerant systems, an air cycle system has emerged as one of alternatives of Freon gas system due to environmentally friendly feature in spite of the inherent low efficiency. To overcome the technical barrier, this study proposed combination of multiple systems as a hybrid cycle to achieve higher efficiency of an air cycle system. The hybrid air cycle adopts a humidity control units such as an adsorber and a desorber to obtain the cooling effect from latent heat as well as sensible heat. To investigate the efficacy of the hybrid air cycle, the cooling performance of a hybrid air cycle is investigated analytically and experimentally. From the simulation result, it is found that COP of the hybrid air cycle is two times higher than that of the conventional air cycle. The experiments are conducted on the performance of the desiccant system according to the rotation speed in the system and displayed the feasibility of the key element in the hybrid air cycle system. From the results, it is shown that the system efficiency can be enhanced by utilization of the exhausted heat through the ambient heat exchanger with advantage of controlling the humidity by the desiccant rotor.

  14. Solid desiccant dehumidification systems for residential applications

    NASA Astrophysics Data System (ADS)

    Marciniak, T. J.; Grolmes, M. A.; Epstein, M.

    1985-03-01

    It is shown that gas regenerated desiccant dehumidifiers (GRDD) can be economically superior to vapor compression units in the hot, humid climates of the southeast and south. Altough the first cost of a GRDD unit is significantly higher than a vapor compression dehumidifier, reduced operating costs and savings in the installed cost of smaller air-conditioning units can provide an economic means for effective humidity control. The economic benefits are dependent upon: (1) the number of hours of dehumidifier operation per year; and (2) electricity and natural gas cost differentials. Of secondary importance is the coefficient of performance (COP) of the units. Recommendations for additional research and development for a commercial GRDD product line are outlined.

  15. Assessment of solar-assisted gas-fired heat pump systems

    NASA Technical Reports Server (NTRS)

    Lansing, F. L.

    1981-01-01

    As a possible application for the Goldstone Energy Project, the performance of a 10 ton heat pump unit using a hybrid solar gas energy source was evaluated in an effort to optimize the solar collector size. The heat pump system is designed to provide all the cooling and/or heating requirements of a selected office building. The system performance is to be augmented in the heating mode by utilizing the waste heat from the power cycle. A simplified system analysis is described to assess and compute interrrelationships of the engine, heat pump, and solar and building performance parameters, and to optimize the solar concentrator/building area ratio for a minimum total system cost. In addition, four alternative heating cooling systems, commonly used for building comfort, are described; their costs are compared, and are found to be less competitive with the gas solar heat pump system at the projected solar equipment costs.

  16. Economic analysis of solar assisted absorption chiller for a commercial building

    NASA Astrophysics Data System (ADS)

    Antonyraj, Gnananesan

    Dwindling fossil fuels coupled with changes in global climate intensified the drive to make use of renewable energy resources that have negligible impact on the environment. In this attempt, the industrial community produced various devices and systems to make use of solar energy for heating and cooling of building space as well as generate electric power. The most common components employed for collection of solar energy are the flat plate and evacuated tube collectors that produce hot water that can be employed for heating the building space. In order to cool the building, the absorption chiller is commonly employed that requires hot water at high temperatures for its operation. This thesis deals with economic analysis of solar collector and absorption cooling system to meet the building loads of a commercial building located in Chattanooga, Tennessee. Computer simulations are employed to predict the hourly building loads and performance of the flat plate and evacuated tube solar collectors using the hourly weather data. The key variables affecting the economic evaluation of such system are identified and the influence of these parameters is presented. The results of this investigation show that the flat plate solar collectors yield lower payback period compared to the evacuated tube collectors and economic incentives offered by the local and federal agencies play a major role in lowering the payback period.

  17. Liquid Desiccant in Air Conditioners: Nano-Engineered Porous Hollow Fiber Membrane-Based Air Conditioning System

    SciTech Connect

    2010-09-02

    BEETIT Project: UTRC is developing an air conditioning system that is optimized for use in warm and humid climates. UTRC’s air conditioning system integrates a liquid drying agent or desiccant and a traditional vapor compression system found in 90% of air conditioners. The drying agent reduces the humidity in the air before it is cooled, using less energy. The technology uses a membrane as a barrier between the air and the liquid salt stream allowing only water vapor to pass through and not the salt molecules. This solves an inherent problem with traditional liquid desiccant systems—carryover of the liquid drying agent into the conditioned air stream—which eliminates corrosion and health issues

  18. Zero Energy Communities with Central Solar Plants using Liquid Desiccants and Local Storage: Preprint

    SciTech Connect

    Burch, J.; Woods, J.; Kozubal, E.; Boranian, A.

    2012-08-01

    The zero energy community considered here consists of tens to tens-of-thousands of residences coupled to a central solar plant that produces all the community's electrical and thermal needs. A distribution network carries fluids to meet the heating and cooling loads. Large central solar systems can significantly reduce cost of energy vs. single family systems, and they enable economical seasonal heat storage. However, the thermal distribution system is costly. Conventional district heating/cooling systems use a water/glycol solution to deliver sensible energy. Piping is sized to meet the peak instantaneous load. A new district system introduced here differs in two key ways: (i) it continuously distributes a hot liquid desiccant (LD) solution to LD-based heating and cooling equipment in each home; and (ii) it uses central and local storage of both LD and heat to reduce flow rates to meet average loads. Results for piping sizes in conventional and LD thermal communities show that the LD zero energy community reduces distribution piping diameters meeting heating loads by {approx}5X and meeting cooling loads by {approx}8X for cooling, depending on climate.

  19. Annual DOE active solar heating and cooling contractors' review meeting. Premeeting proceedings and project summaries

    SciTech Connect

    None,

    1981-09-01

    Ninety-three project summaries are presented which discuss the following aspects of active solar heating and cooling: Rankine solar cooling systems; absorption solar cooling systems; desiccant solar cooling systems; solar heat pump systems; solar hot water systems; special projects (such as the National Solar Data Network, hybrid solar thermal/photovoltaic applications, and heat transfer and water migration in soils); administrative/management support; and solar collector, storage, controls, analysis, and materials technology. (LEW)

  20. Genetic Analysis of Desiccation Tolerance in Saccharomyces cerevisiae

    PubMed Central

    Calahan, Dean; Dunham, Maitreya; DeSevo, Chris; Koshland, Douglas E.

    2011-01-01

    Desiccation tolerance, the ability to survive nearly total dehydration, is a rare strategy for survival and reproduction observed in all taxa. However, the mechanism and regulation of this phenomenon are poorly understood. Correlations between desiccation tolerance and potential effectors have been reported in many species, but their physiological significance has not been established in vivo. Although the budding yeast Saccharomyces cerevisiae exhibits extreme desiccation tolerance, its usefulness has been hampered by an inability to reduce tolerance more than a few fold by physiological or genetic perturbations. Here we report that fewer than one in a million yeast cells from low-density logarithmic cultures survive desiccation, while 20–40% of cells from saturated cultures survive. Using this greatly expanded metric, we show that mutants defective in trehalose biosynthesis, hydrophilins, responses to hyperosmolarity, and hypersalinity, reactive oxygen species (ROS) scavenging and DNA damage repair nevertheless retain wild-type levels of desiccation tolerance, suggesting that this trait involves a unique constellation of stress factors. A genome-wide screen for mutants that render stationary cells as sensitive as log phase cells identifies only mutations that block respiration. Respiration as a prerequisite for acquiring desiccation tolerance is corroborated by respiration inhibition and by growth on nonfermentable carbon sources. Suppressors bypassing the respiration requirement for desiccation tolerance reveal at least two pathways, one of which, involving the Mediator transcription complex, is associated with the shift from fermentative to respiratory metabolism. Further study of these regulators and their targets should provide important clues to the sensors and effectors of desiccation tolerance. PMID:21840858

  1. Genome structure of bdelloid rotifers: shaped by asexuality or desiccation?

    PubMed

    Gladyshev, Eugene A; Arkhipova, Irina R

    2010-01-01

    Bdelloid rotifers are microscopic invertebrate animals best known for their ancient asexuality and the ability to survive desiccation at any life stage. Both factors are expected to have a profound influence on their genome structure. Recent molecular studies demonstrated that, although the gene-rich regions of bdelloid genomes are organized as colinear pairs of closely related sequences and depleted in repetitive DNA, subtelomeric regions harbor diverse transposable elements and horizontally acquired genes of foreign origin. Although asexuality is expected to result in depletion of deleterious transposons, only desiccation appears to have the power to produce all the uncovered genomic peculiarities. Repair of desiccation-induced DNA damage would require the presence of a homologous template, maintaining colinear pairs in gene-rich regions and selecting against insertion of repetitive DNA that might cause chromosomal rearrangements. Desiccation may also induce a transient state of competence in recovering animals, allowing them to acquire environmental DNA. Even if bdelloids engage in rare or obscure forms of sexual reproduction, all these features could still be present. The relative contribution of asexuality and desiccation to genome organization may be clarified by analyzing whole-genome sequences and comparing foreign gene and transposon content in species which lost the ability to survive desiccation.

  2. Hydraulic conductivity of desiccated geosynthetic clay liners

    SciTech Connect

    Boardman, B.T.; Daniel, D.E.

    1996-03-01

    Large-scale tests were performed to determine the effect of a cycle of wetting and drying on the hydraulic conductivity of several geosynthetic clay liners (GCLs). The GCLs were covered with 0.6 m of pea gravel and permeated with water. After steady seepage had developed, the water was drained away, and the GCL was desiccated by circulating heated air through the overlying gravel. The drying caused severe cracking in the bentonite component of the GCLs. The GCLs were again permeated with water. As the cracked bentonite hydrated and swelled, the hydraulic conductivity slowly decreased from an initially high value. The long-term, steady value of hydraulic conductivity after the wetting and drying cycle was found to be essentially the same as the value for the undesiccated GCL. It is concluded that GCLs possess the ability to self-heal after a cycle of wetting and drying, which is important for applications in which there may be alternate wetting and drying of a hydraulic barrier (e.g. within a landfill final cover).

  3. The modern office environment desiccates the eyes?

    PubMed

    Wolkoff, P; Nøjgaard, J K; Franck, C; Skov, P

    2006-08-01

    Eye irritation is a common complaint in the office environment. The purpose of this overview is to merge knowledge within indoor air science, ophthalmology, and occupational health to promote understanding eye irritation symptomatology, the cause of which is still partly unknown. High periocular relative humidity appears to protect the pre-corneal tear film against desiccation and sensory irritating pollutants and reduces the development of eye irritation symptoms. This is particularly relevant for intensive computer work, where the pre-corneal tear film is altered resulting in dry spot formation and eye dryness, in addition to enhanced susceptibility towards sensory irritating pollutants. The workplace, thermal conditions, and work schedule (including breaks) should be planned in such a way to help maintain a normal eye blink frequency to minimize alterations of the pre-corneal tear film. The role of relative humidity on eye irritation symptoms should not be underestimated. Multiple short breaks are justified by the beneficial effect on the pre-corneal tear film. In addition, longer breaks in tasks, which require demanding visual work, should be considered. In addition, air temperature as well as certain alkene oxidation products by ozone may worsen eye irritation symptoms, but the latter factor may be smaller at higher relative humidity.

  4. Prototype solar heating and cooling systems, including potable hot water

    NASA Technical Reports Server (NTRS)

    Bloomquist, D.; Oonk, R. L.

    1977-01-01

    Progress made in the development, delivery, and support of two prototype solar heating and cooling systems including potable hot water is reported. The system consists of the following subsystems: collector, auxiliary heating, potable hot water, storage, control, transport, and government-furnished site data acquisition. A comparison of the proposed Solaron Heat Pump and Solar Desiccant Heating and Cooling Systems, installation drawings, data on the Akron House at Akron, Ohio, and other program activities are included.

  5. Developing sporophytes transition from an inducible to a constitutive ecological strategy of desiccation tolerance in the moss Aloina ambigua: effects of desiccation on fitness

    PubMed Central

    Stark, Lloyd R.; Brinda, John C.

    2015-01-01

    Background and Aims Two ecological strategies of desiccation tolerance exist in plants, constitutive and inducible. Because of difficulties in culturing sporophytes, very little is known about desiccation tolerance in this generation and how desiccation affects sexual fitness. Methods Cultured sporophytes and vegetative shoots from a single genotype of the moss Aloina ambigua raised in the laboratory were tested for their strategy of desiccation tolerance by desiccating the shoot–sporophyte complex and vegetative shoots at different intensities, and comparing outcomes with those of undried shoot–sporophyte complexes and vegetative shoots. By using a dehardened clonal line, the effects of field, age and genetic variance among plants were removed. Key Results The gametophyte and embryonic sporophyte were found to employ a predominantly inducible strategy of desiccation tolerance, while the post-embryonic sporophyte was found to employ a moderately constitutive strategy of desiccation tolerance. Further, desiccation reduced sporophyte fitness, as measured by sporophyte mass, seta length and capsule size. However, the effects of desiccation on sporophyte fitness were reduced if the stress occurred during embryonic development as opposed to postembryonic desiccation. Conclusions The effects of desiccation on dehardened sporophytes of a bryophyte are shown for the first time. The transition from one desiccation tolerance strategy to the other in a single structure or generation is shown for only the second time in plants and for the first time in bryophytes. Finding degrees of inducible strategies of desiccation tolerance in different life phases prompts the formulation of a continuum hypothesis of ecological desiccation tolerance in mosses, where desiccation tolerance is not an either/or phenomenon, but varies in degree along a gradient of ecological inducibility. PMID:25578378

  6. Desiccation resistance in tropical insects: causes and mechanisms underlying variability in a Panama ant community.

    PubMed

    Bujan, Jelena; Yanoviak, Stephen P; Kaspari, Michael

    2016-09-01

    Desiccation resistance, the ability of an organism to reduce water loss, is an essential trait in arid habitats. Drought frequency in tropical regions is predicted to increase with climate change, and small ectotherms are often under a strong desiccation risk. We tested hypotheses regarding the underexplored desiccation potential of tropical insects. We measured desiccation resistance in 82 ant species from a Panama rainforest by recording the time ants can survive desiccation stress. Species' desiccation resistance ranged from 0.7 h to 97.9 h. We tested the desiccation adaptation hypothesis, which predicts higher desiccation resistance in habitats with higher vapor pressure deficit (VPD) - the drying power of the air. In a Panama rainforest, canopy microclimates averaged a VPD of 0.43 kPa, compared to a VPD of 0.05 kPa in the understory. Canopy ants averaged desiccation resistances 2.8 times higher than the understory ants. We tested a number of mechanisms to account for desiccation resistance. Smaller insects should desiccate faster given their higher surface area to volume ratio. Desiccation resistance increased with ant mass, and canopy ants averaged 16% heavier than the understory ants. A second way to increase desiccation resistance is to carry more water. Water content was on average 2.5% higher in canopy ants, but total water content was not a good predictor of ant desiccation resistance or critical thermal maximum (CT max), a measure of an ant's thermal tolerance. In canopy ants, desiccation resistance and CT max were inversely related, suggesting a tradeoff, while the two were positively correlated in understory ants. This is the first community level test of desiccation adaptation hypothesis in tropical insects. Tropical forests do contain desiccation-resistant species, and while we cannot predict those simply based on their body size, high levels of desiccation resistance are always associated with the tropical canopy.

  7. Desiccation resistance in tropical insects: causes and mechanisms underlying variability in a Panama ant community.

    PubMed

    Bujan, Jelena; Yanoviak, Stephen P; Kaspari, Michael

    2016-09-01

    Desiccation resistance, the ability of an organism to reduce water loss, is an essential trait in arid habitats. Drought frequency in tropical regions is predicted to increase with climate change, and small ectotherms are often under a strong desiccation risk. We tested hypotheses regarding the underexplored desiccation potential of tropical insects. We measured desiccation resistance in 82 ant species from a Panama rainforest by recording the time ants can survive desiccation stress. Species' desiccation resistance ranged from 0.7 h to 97.9 h. We tested the desiccation adaptation hypothesis, which predicts higher desiccation resistance in habitats with higher vapor pressure deficit (VPD) - the drying power of the air. In a Panama rainforest, canopy microclimates averaged a VPD of 0.43 kPa, compared to a VPD of 0.05 kPa in the understory. Canopy ants averaged desiccation resistances 2.8 times higher than the understory ants. We tested a number of mechanisms to account for desiccation resistance. Smaller insects should desiccate faster given their higher surface area to volume ratio. Desiccation resistance increased with ant mass, and canopy ants averaged 16% heavier than the understory ants. A second way to increase desiccation resistance is to carry more water. Water content was on average 2.5% higher in canopy ants, but total water content was not a good predictor of ant desiccation resistance or critical thermal maximum (CT max), a measure of an ant's thermal tolerance. In canopy ants, desiccation resistance and CT max were inversely related, suggesting a tradeoff, while the two were positively correlated in understory ants. This is the first community level test of desiccation adaptation hypothesis in tropical insects. Tropical forests do contain desiccation-resistant species, and while we cannot predict those simply based on their body size, high levels of desiccation resistance are always associated with the tropical canopy. PMID:27648242

  8. The limits and frontiers of desiccation-tolerant life.

    PubMed

    Alpert, Peter

    2005-11-01

    Drying to equilibrium with the air is lethal to most species of animals and plants, making drought (i.e., low external water potential) a central problem for terrestrial life and a major cause of agronomic failure and human famine. Surprisingly, a wide taxonomic variety of animals, microbes, and plants do tolerate complete desiccation, defined as water content below 0.1 g H(2)O g(-1) dry mass. Species in five phyla of animals and four divisions of plants contain desiccation-tolerant adults, juveniles, seeds, or spores. There seem to be few inherent limits on desiccation tolerance, since tolerant organisms can survive extremely intense and prolonged desiccation. There seems to be little phylogenetic limitation of tolerance in plants but may be more in animals. Physical constraints may restrict tolerance of animals without rigid skeletons and to plants shorter than 3 m. Physiological constraints on tolerance in plants may include control by hormones with multiple effects that could link tolerance to slow growth. Tolerance tends to be lower in organisms from wetter habitats, and there may be selection against tolerance when water availability is high. Our current knowledge of limits to tolerance suggests that they pose few obstacles to engineering tolerance in prokaryotes and in isolated cells and tissues, and there has already been much success on this scientific frontier of desiccation tolerance. However, physical and physiological constraints and perhaps other limits may explain the lack of success in extending tolerance to whole, desiccation-sensitive, multicellular animals and plants. Deeper understanding of the limits to desiccation tolerance in living things may be needed to cross this next frontier.

  9. Performance characteristics of a commercially available gas-fired desiccant system

    SciTech Connect

    Jalalzadeh-Azar, A.A.; Steele, W.G.; Hodge, B.K.

    2000-07-01

    Performance characteristics of a commercially available desiccant dehumidification system are experimentally determined. The system under consideration utilizes a rotary desiccant wheel that is regenerated by a gas-fired hot water heating system. The constituents of the study are (1) evaluation of the issues critical to accurate data acquisition and (2) examination of the sensitivities of system performance parameters to perturbations in certain design set points. The effectiveness of a mixing device in providing a uniform-property supply air is demonstrated, and its importance is discussed from the standpoint of the uncertainties in the experimental data. Sample data are presented to demonstrate the temporal variation of the supply air properties, temperature and humidity ratio, when the regeneration heating system is operated by an on-off control scheme. Two design variables, regeneration-to-process air ratio and regeneration temperature, are allowed to deviate from their respective set points, while the responses of the system performance parameters (an overall coefficient of performance [COP] for dehumidification, a local COP, the moisture removal capacity, and the effective equivalent cooling capacity) are observed. The local COP is a means for evaluating the efficiency of the dehumidification process based on the net heat output of the regeneration heating system. The distinction between these definitions for COP is useful in identifying the regeneration heating system or the dehumidification process as the potential source of inefficiency in the overall system performance.

  10. Heat-activated cooling devices: A guidebook for general audiences

    SciTech Connect

    Wiltsee, G.

    1994-02-01

    Heat-activated cooling is refrigeration or air conditioning driven by heat instead of electricity. A mill or processing facility can us its waste fuel to air condition its offices or plant; using waste fuel in this way can save money. The four basic types of heat-activated cooling systems available today are absorption cycle, desiccant system, steam jet ejector, and steam turbine drive. Each is discussed, along with cool storage and biomass boilers. Steps in determining the feasibility of heat-activated cooling are discussed, as are biomass conversion, system cost and integration, permits, and contractor selection. Case studies are given.

  11. Insect capa neuropeptides impact desiccation and cold tolerance

    PubMed Central

    Terhzaz, Selim; Teets, Nicholas M.; Cabrero, Pablo; Henderson, Louise; Ritchie, Michael G.; Nachman, Ronald J.; Dow, Julian A. T.; Denlinger, David L.; Davies, Shireen-A.

    2015-01-01

    The success of insects is linked to their impressive tolerance to environmental stress, but little is known about how such responses are mediated by the neuroendocrine system. Here we show that the capability (capa) neuropeptide gene is a desiccation- and cold stress-responsive gene in diverse dipteran species. Using targeted in vivo gene silencing, physiological manipulations, stress-tolerance assays, and rationally designed neuropeptide analogs, we demonstrate that the Drosophila melanogaster capa neuropeptide gene and its encoded peptides alter desiccation and cold tolerance. Knockdown of the capa gene increases desiccation tolerance but lengthens chill coma recovery time, and injection of capa peptide analogs can reverse both phenotypes. Immunohistochemical staining suggests that capa accumulates in the capa-expressing Va neurons during desiccation and nonlethal cold stress but is not released until recovery from each stress. Our results also suggest that regulation of cellular ion and water homeostasis mediated by capa peptide signaling in the insect Malpighian (renal) tubules is a key physiological mechanism during recovery from desiccation and cold stress. This work augments our understanding of how stress tolerance is mediated by neuroendocrine signaling and illustrates the use of rationally designed peptide analogs as agents for disrupting protective stress tolerance. PMID:25730885

  12. Insect capa neuropeptides impact desiccation and cold tolerance.

    PubMed

    Terhzaz, Selim; Teets, Nicholas M; Cabrero, Pablo; Henderson, Louise; Ritchie, Michael G; Nachman, Ronald J; Dow, Julian A T; Denlinger, David L; Davies, Shireen-A

    2015-03-01

    The success of insects is linked to their impressive tolerance to environmental stress, but little is known about how such responses are mediated by the neuroendocrine system. Here we show that the capability (capa) neuropeptide gene is a desiccation- and cold stress-responsive gene in diverse dipteran species. Using targeted in vivo gene silencing, physiological manipulations, stress-tolerance assays, and rationally designed neuropeptide analogs, we demonstrate that the Drosophila melanogaster capa neuropeptide gene and its encoded peptides alter desiccation and cold tolerance. Knockdown of the capa gene increases desiccation tolerance but lengthens chill coma recovery time, and injection of capa peptide analogs can reverse both phenotypes. Immunohistochemical staining suggests that capa accumulates in the capa-expressing Va neurons during desiccation and nonlethal cold stress but is not released until recovery from each stress. Our results also suggest that regulation of cellular ion and water homeostasis mediated by capa peptide signaling in the insect Malpighian (renal) tubules is a key physiological mechanism during recovery from desiccation and cold stress. This work augments our understanding of how stress tolerance is mediated by neuroendocrine signaling and illustrates the use of rationally designed peptide analogs as agents for disrupting protective stress tolerance.

  13. Desiccation tolerance of protoplasts isolated from pea embryos.

    PubMed

    Xiao, L; Koster, K L

    2001-11-01

    To facilitate studies of desiccation tolerance at the cellular level, a technique to isolate protoplasts from desiccation-tolerant pea (Pisum sativum L. cv. Alaska) embryos has been developed. Using FDA (fluorescein diacetate) as a probe, viability of the protoplasts was investigated before and after drying to determine whether the protoplasts could survive desiccation in a manner similar to the tissue from which they were isolated. Protoplasts were isolated from 12 h imbibed pea axes, suspended in several different sugar solutions, then dried to water contents less than 0.2 g H(2)O g(-1) DW. Protoplasts only survived drying if the rate was rapid (<2 h), while slow drying (24 h) was lethal. Maximal survival (75%) was obtained after drying protoplasts with a mixture of sucrose and raffinose, while pure sucrose and trehalose were somewhat less effective protectants. Low survival was obtained after drying protoplasts with monosaccharides and pure raffinose. Protoplasts isolated from germinated seedlings did not survive dehydration below 0.2 g H(2)O g(-1) DW. Transmission electron microscopy revealed that dried desiccation-tolerant protoplasts appeared shrunken, with folded membranes, while dried protoplasts from sensitive tissue had disrupted membranes. While isolated protoplasts maintained some of the desiccation tolerance of orthodox seeds, their inability to survive complete drying and their sensitivity to drying rate is similar to the behaviour of recalcitrant embryos.

  14. Increasing intracellular trehalose is sufficient to confer desiccation tolerance to Saccharomyces cerevisiae.

    PubMed

    Tapia, Hugo; Young, Lindsey; Fox, Douglas; Bertozzi, Carolyn R; Koshland, Douglas

    2015-05-12

    Diverse organisms capable of surviving desiccation, termed anhydrobiotes, include species from bacteria, yeast, plants, and invertebrates. However, most organisms are sensitive to desiccation, likely due to an assortment of different stresses such as protein misfolding and aggregation, hyperosmotic stress, membrane fracturing, and changes in cell volume and shape leading to an overcrowded cytoplasm and metabolic arrest. The exact stress(es) that cause lethality in desiccation-sensitive organisms and how the lethal stresses are mitigated in desiccation-tolerant organisms remain poorly understood. The presence of trehalose in anhydrobiotes has been strongly correlated with desiccation tolerance. In the yeast Saccharomyces cerevisiae, trehalose is essential for survival after long-term desiccation. Here, we establish that the elevation of intracellular trehalose in dividing yeast by its import from the media converts yeast from extreme desiccation sensitivity to a high level of desiccation tolerance. This trehalose-induced tolerance is independent of utilization of trehalose as an energy source, de novo synthesis of other stress effectors, or the metabolic effects of trehalose biosynthetic intermediates, indicating that a chemical property of trehalose is directly responsible for desiccation tolerance. Finally, we demonstrate that elevated intracellular maltose can also make dividing yeast tolerant to short-term desiccation, indicating that other disaccharides have stress effector activity. However, trehalose is much more effective than maltose at conferring tolerance to long-term desiccation. The effectiveness and sufficiency of trehalose as an antagonizer of desiccation-induced damage in yeast emphasizes its potential to confer desiccation tolerance to otherwise sensitive organisms.

  15. Open cycle liquid desiccant dehumidifier and hybrid solar/electric absorption refrigeration system. Annual report, January 1993--December 1993. Calendar year 1993

    SciTech Connect

    Nimmo, B.G.; Thornbloom, M.D.

    1995-04-01

    This annual report presents work performed during calendar year 1993 by the Florida Solar Energy Center under contract to the US Department of Energy. Two distinctively different solar powered indoor climate control systems were analyzed: the open cycle liquid desiccant dehumidifier, and an improved efficiency absorption system which may be fired by flat plate solar collectors. Both tasks represent new directions relative to prior FSEC research in Solar Cooling and Dehumidification.

  16. Desiccation resistance and persistence of Cronobacter species in infant formula.

    PubMed

    Osaili, T; Forsythe, S

    2009-12-31

    Cronobacter is a newly described genus which includes opportunistic pathogens formerly known as 'Enterobacter sakazakii'. These organisms have been isolated from a wide variety of sources, including powdered infant formula (PIF). This review focuses on the desiccation survival of Cronobacter, and its relevance to vehicles of infection. Due to its probable natural habitat of plant material, the organism has an array of survival mechanisms which includes resistance to desiccation and osmotic stresses. The organism can survive for long periods of time (>2years) in the desiccated state, and can be recovered from a large number of powdered foods in addition to powdered infant formula. On reconstitution, the organism may rapidly multiply and present a risk to immunocompromised infants. It is expected that an improved understanding of the nature of Cronobacter persistence may aid in further improved control measures and eliminate the bacterium from the critical food production environments.

  17. Function of desiccate in gustatory sensilla of drosophila melanogaster

    PubMed Central

    Kawano, Takeshi; Ryuda, Masasuke; Matsumoto, Hitoshi; Ochiai, Masanori; Oda, Yasunori; Tanimura, Teiichi; Csikos, Gyorge; Moriya, Megumi; Hayakawa, Yoichi

    2015-01-01

    Desiccate (Desi), initially discovered as a gene expressing in the epidermis of Drosophila larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that Desi is expressed in two different types of non-neuronal cells of the labellum, the epidermis and thecogen accessory cells. Labellar Desi expression was significantly elevated under arid conditions, accompanied by an increase in water ingestion by adults. Desi overexpression also promoted water ingestion. In contrast, a knockdown of Desi expression reduced feeding as well as water ingestion due to a drastic decrease in the gustatory sensillar sensitivity for all tested tastants. These results indicate that Desi helps protect insects from desiccation damage by not only preventing dehydration through the integument but also accelerating water ingestion via elevated taste sensitivities of the sensilla. PMID:26610608

  18. Function of desiccate in gustatory sensilla of drosophila melanogaster.

    PubMed

    Kawano, Takeshi; Ryuda, Masasuke; Matsumoto, Hitoshi; Ochiai, Masanori; Oda, Yasunori; Tanimura, Teiichi; Csikos, Gyorge; Moriya, Megumi; Hayakawa, Yoichi

    2015-11-27

    Desiccate (Desi), initially discovered as a gene expressing in the epidermis of Drosophila larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that Desi is expressed in two different types of non-neuronal cells of the labellum, the epidermis and thecogen accessory cells. Labellar Desi expression was significantly elevated under arid conditions, accompanied by an increase in water ingestion by adults. Desi overexpression also promoted water ingestion. In contrast, a knockdown of Desi expression reduced feeding as well as water ingestion due to a drastic decrease in the gustatory sensillar sensitivity for all tested tastants. These results indicate that Desi helps protect insects from desiccation damage by not only preventing dehydration through the integument but also accelerating water ingestion via elevated taste sensitivities of the sensilla.

  19. Bacterial survival responses to extreme desiccation and high humidity

    NASA Astrophysics Data System (ADS)

    Yang, Yinjie; Yokobori, Shinichi; Yamagishi, Akihiko

    The presence of water is thought to be essential for life and strongly considered in life searching operation on extraterrestrial planets. In this study we show different survival responses of bacterial species to water availability and temperatures (25, 4 and - 70 o C). At these temperatures, E.coli lost viability much faster under extreme desiccation than under high humidity. Deinococcus radiodurans exhibited much higher survival rate under desiccation than under high humidity at 25 o C, while its survivals under desiccation and high humidity increased to the same level at 4 and - 70 o C. Bacillus pumilus spores generally survived well under all tested conditions. Water is favorable for the survival of most microorganisms but not a "safeguard" for all microorganisms. Microbial survival at low temperatures may not be affected by water availability. Water absence should not preclude us from seeking life on other planets.

  20. Using liquid desiccant as a regenerable filter for capturing and deactivating contaminants

    DOEpatents

    Slayzak, Steven J.; Anderson, Ren S.; Judkoff, Ronald D.; Blake, Daniel M.; Vinzant, Todd B.; Ryan, Joseph P.

    2007-12-11

    A method, and systems for implementing such method, for purifying and conditioning air of weaponized contaminants. The method includes wetting a filter packing media with a salt-based liquid desiccant, such as water with a high concentration of lithium chloride. Air is passed through the wetted filter packing media and the contaminants in are captured with the liquid desiccant while the liquid desiccant dehumidifies the air. The captured contaminants are then deactivated in the liquid desiccant, which may include heating the liquid desiccant. The liquid desiccant is regenerated by applying heat to the liquid desiccant and then removing moisture. The method includes repeating the wetting with the regenerated liquid desiccant which provides a regenerable filtering process that captures and deactivates contaminants on an ongoing basis while also conditioning the air. The method may include filtration effectiveness enhancement by electrostatic or inertial means.

  1. Traits underpinning desiccation resistance explain distribution patterns of terrestrial isopods.

    PubMed

    Dias, André T C; Krab, Eveline J; Mariën, Janine; Zimmer, Martin; Cornelissen, Johannes H C; Ellers, Jacintha; Wardle, David A; Berg, Matty P

    2013-07-01

    Predicted changes in soil water availability regimes with climate and land-use change will impact the community of functionally important soil organisms, such as macro-detritivores. Identifying and quantifying the functional traits that underlie interspecific differences in desiccation resistance will enhance our ability to predict both macro-detritivore community responses to changing water regimes and the consequences of the associated species shifts for organic matter turnover. Using path analysis, we tested (1) how interspecific differences in desiccation resistance among 22 northwestern European terrestrial isopod species could be explained by three underlying traits measured under standard laboratory conditions, namely, body ventral surface area, water loss rate and fatal water loss; (2) whether these relationships were robust to contrasting experimental conditions and to the phylogenetic relatedness effects being excluded; (3) whether desiccation resistance and hypothesized underlying traits could explain species distribution patterns in relation to site water availability. Water loss rate and (secondarily) fatal water loss together explained 90% of the interspecific variation in desiccation resistance. Our path model indicated that body surface area affects desiccation resistance only indirectly via changes in water loss rate. Our results also show that soil moisture determines isopod species distributions by filtering them according to traits underpinning desiccation resistance. These findings reveal that it is possible to use functional traits measured under standard conditions to predict soil biota responses to water availability in the field over broad spatial scales. Taken together, our results demonstrate an increasing need to generate mechanistic models to predict the effect of global changes on functionally important organisms.

  2. Bactericidal activity of TiO[sub 2] photocatalyst in aqueous media. Toward a solar-assisted water disinfection system

    SciTech Connect

    Wei, C.; Lin, W.Y.; Zainal, Z.; Williams, N.E.; Zhu, K.; Kruzic, A.P.; Smith, R.L.; Rajeshwar, K. )

    1994-05-01

    Irradiation of suspensions of Escherichia coli ([approximately] 10[sup 6] cells/mL) and TiO[sub 2] (anatase) with UV-visible light of wave-lengths longer than 380 nm resulted in the killing of the bacteria within minutes. Oxygen was found to be a prerequisite for the bactericidal properties of the photocatalyst. Bacterial killing was found to adhere to first-order kinetics. The rate constant was proportional to the square root of the concentration of TiO[sub 2] and proportional to the incident light intensity in the range [approximately] 180- [approximately] 1660 [mu]E s[sup [minus]1] m[sup [minus]2]. The trends in these simulated laboratory experiments were mimicked by outdoor tests conducted under the summer noonday sun in Texas. The implications of these results as well as those of previous investigations in terms of practical applicability to solar-assisted water treatment and disinfection at remote sites are discussed relative to water technologies currently considered as viable as alternatives to chlorination. 24 refs., 8 figs.

  3. Efficient mineralization of the antibiotic trimethoprim by solar assisted photoelectro-Fenton process driven by a photovoltaic cell.

    PubMed

    Zhang, Yanyu; Wang, Aimin; Tian, Xiujun; Wen, Zhenjun; Lv, Hanjiao; Li, Desheng; Li, Jiuyi

    2016-11-15

    In this study, a novel self-sustainable solar assisted photoelectro-Fenton (SPEF) system driven by a solar photovoltaic cell was developed for the efficient mineralization of antibiotic trimethoprim (TMP) in water. A comparative degradation of 200mgL(-1) TMP by RuO2/Ti anodic oxidation (AO), anodic oxidation with H2O2 electrogeneration (AO-H2O2), electro-Fenton (EF) and SPEF was investigated. SPEF was proved to exhibit the highest oxidation power, i.e., more than 80% TOC was removed after 360min SPEF treatment of 200mgL(-1) of TMP under optimal conditions at pH 3.0, 1.0mM Fe(2+) and 18mAcm(-2). Influences of current density, pH, initial Fe(2+) and initial TMP concentration on SPEF process were also studied. Ten aromatic intermediates generated from hydroxylation, carbonylation and demethylation reactions were identified using UPLC-QTOF-MS/MS system during the SPEF treatment, together with three carboxylic acids (oxamic, oxalic and formic acids) and two inorganic ions (NH4(+) and NO3(-)) measured. Therefore, a reasonable pathway of TMP degradation in SPEF process was proposed.

  4. Efficient mineralization of the antibiotic trimethoprim by solar assisted photoelectro-Fenton process driven by a photovoltaic cell.

    PubMed

    Zhang, Yanyu; Wang, Aimin; Tian, Xiujun; Wen, Zhenjun; Lv, Hanjiao; Li, Desheng; Li, Jiuyi

    2016-11-15

    In this study, a novel self-sustainable solar assisted photoelectro-Fenton (SPEF) system driven by a solar photovoltaic cell was developed for the efficient mineralization of antibiotic trimethoprim (TMP) in water. A comparative degradation of 200mgL(-1) TMP by RuO2/Ti anodic oxidation (AO), anodic oxidation with H2O2 electrogeneration (AO-H2O2), electro-Fenton (EF) and SPEF was investigated. SPEF was proved to exhibit the highest oxidation power, i.e., more than 80% TOC was removed after 360min SPEF treatment of 200mgL(-1) of TMP under optimal conditions at pH 3.0, 1.0mM Fe(2+) and 18mAcm(-2). Influences of current density, pH, initial Fe(2+) and initial TMP concentration on SPEF process were also studied. Ten aromatic intermediates generated from hydroxylation, carbonylation and demethylation reactions were identified using UPLC-QTOF-MS/MS system during the SPEF treatment, together with three carboxylic acids (oxamic, oxalic and formic acids) and two inorganic ions (NH4(+) and NO3(-)) measured. Therefore, a reasonable pathway of TMP degradation in SPEF process was proposed. PMID:27434735

  5. Is cooling still cool?

    PubMed

    Subramaniam, Ashwin; Tiruvoipati, Ravindranath; Botha, John

    2015-03-01

    Therapeutic hypothermia (TH), where patients are cooled to between 32°C and 36°C for a period of 12-24 hours and then gradually rewarmed, may reduce the risk of ischemic injury to cerebral tissue following a period of insufficient blood flow. This strategy of TH could improve mortality and neurological function in patients who have experienced out-of-hospital cardiac arrest (OOHCA). The necessity of TH in OOHCA was challenged in late 2013 by a fascinating and potentially practice changing publication, which found that targeting a temperature of 36°C had similar outcomes to cooling patients to 33°C. This article reviews the current literature and summarizes the uncertainties and questions raised when considering cooling of patients at risk of hypoxic brain injury. Irrespective of whether TH or targeted temperature management is deployed in patients at risk of hypoxic brain injury, it would seem that avoiding hyperpyrexia is important and that a more rigorous approach to neurological evaluation is mandated. PMID:25423577

  6. Electron cooling

    NASA Astrophysics Data System (ADS)

    Meshkov, I.; Sidorin, A.

    2004-10-01

    The brief review of the most significant and interesting achievements in electron cooling method, which took place during last two years, is presented. The description of the electron cooling facilities-storage rings and traps being in operation or under development-is given. The applications of the electron cooling method are considered. The following modern fields of the method development are discussed: crystalline beam formation, expansion into middle and high energy electron cooling (the Fermilab Recycler Electron Cooler, the BNL cooler-recuperator, cooling with circulating electron beam, the GSI project), electron cooling in traps, antihydrogen generation, electron cooling of positrons (the LEPTA project).

  7. Stochastic Cooling

    SciTech Connect

    Blaskiewicz, M.

    2011-01-01

    Stochastic Cooling was invented by Simon van der Meer and was demonstrated at the CERN ISR and ICE (Initial Cooling Experiment). Operational systems were developed at Fermilab and CERN. A complete theory of cooling of unbunched beams was developed, and was applied at CERN and Fermilab. Several new and existing rings employ coasting beam cooling. Bunched beam cooling was demonstrated in ICE and has been observed in several rings designed for coasting beam cooling. High energy bunched beams have proven more difficult. Signal suppression was achieved in the Tevatron, though operational cooling was not pursued at Fermilab. Longitudinal cooling was achieved in the RHIC collider. More recently a vertical cooling system in RHIC cooled both transverse dimensions via betatron coupling.

  8. Solar heating and cooling system installed at Leavenworth, Kansas

    NASA Technical Reports Server (NTRS)

    1980-01-01

    A solar heating and cooling is described which is designed to furnish 90 percent of the overall heating load, 70 percent of the cooling load and 100 percent of the domestic hot water load. The building has two floors with a total of 12,000 square feet gross area. The system has 120 flat-plate liquid solar panels with a net area of 2,200 square feet. Five 3 ton Arkla solar assisted absorption units provide the cooling, in conjunction with a 3,000 gallon chilled water storage tank. Two 3,000 gallon storage tanks are provided with one designated for summer use, whereas both tanks are utilized during winter.

  9. Solar heating and cooling system installed at Leavenworth, Kansas

    NASA Astrophysics Data System (ADS)

    1980-06-01

    A solar heating and cooling is described which is designed to furnish 90 percent of the overall heating load, 70 percent of the cooling load and 100 percent of the domestic hot water load. The building has two floors with a total of 12,000 square feet gross area. The system has 120 flat-plate liquid solar panels with a net area of 2,200 square feet. Five 3 ton Arkla solar assisted absorption units provide the cooling, in conjunction with a 3,000 gallon chilled water storage tank. Two 3,000 gallon storage tanks are provided with one designated for summer use, whereas both tanks are utilized during winter.

  10. Colorado State University program for developing, testing, evaluating and optimizing solar heating and cooling systems

    SciTech Connect

    Not Available

    1993-01-07

    This program includes six tasks, including (1) a project measuring the performance of unique solar system components, (2) a project to develop a methodology for determining annual performance ratings of solar domestic hot water systems, (3) a project that will identify, analyze, design, build, and experimentally evaluate SDHW systems incorporating advanced concepts and components, (4) a liquid desiccant cooling system development project, (5) a project that will perform TRNSYS simulations to determine potential energy savings for desiccant cooling systems, especially in humid climates, and (6) a management task. The objectives and progress in each task are described.

  11. Lessons on dehydration tolerance from desiccation tolerant plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Extremophiles: organisms that thrive (a relative term) in environments where conditions are such that the majority of organisms cannot survive. This is not strictly true if one is describing desiccation-tolerant plants, as other plants do grow around them, but it is certainly true that they can surv...

  12. Desiccation-crack-induced salinization in deep clay sediment

    NASA Astrophysics Data System (ADS)

    Baram, S.; Ronen, Z.; Kurtzman, D.; Külls, C.; Dahan, O.

    2013-04-01

    A study on water infiltration and solute transport in a clayey vadose zone underlying a dairy farm waste source was conducted to assess the impact of desiccation cracks on subsurface evaporation and salinization. The study is based on five years of continuous measurements of the temporal variation in the vadose zone water content and on the chemical and isotopic composition of the sediment and pore water in it. The isotopic composition of water stable isotopes (δ18O and δ2H) in water and sediment samples, from the area where desiccation crack networks prevail, indicated subsurface evaporation down to ~ 3.5 m below land surface, and vertical and lateral preferential transport of water, following erratic preferential infiltration events. Chloride (Cl-) concentrations in the vadose zone pore water substantially increased with depth, evidence of deep subsurface evaporation and down flushing of concentrated solutions from the evaporation zones during preferential infiltration events. These observations led to development of a desiccation-crack-induced salinization (DCIS) conceptual model. DCIS suggests that thermally driven convective air flow in the desiccation cracks induces evaporation and salinization in relatively deep sections of the subsurface. This conceptual model supports previous conceptual models on vadose zone and groundwater salinization in fractured rock in arid environments and extends its validity to clayey soils in semi-arid environments.

  13. Desiccation-crack-induced salinization in deep clay sediment

    NASA Astrophysics Data System (ADS)

    Baram, S.; Ronen, Z.; Kurtzman, D.; Küells, C.; Dahan, O.

    2012-11-01

    A study on water infiltration and solute transport in a clayey vadose zone underlying a dairy farm waste source was conducted to assess the impact of desiccation cracks on subsurface evaporation and salinization. The study is based on five years of continuous measurements of the temporal variation in the vadose zone water-content and on the chemical and isotopic composition of the sediment and pore-water in it. The isotopic composition of water stable isotopes (δ18O and δ2H) in water and sediment samples, from the area where desiccation crack networks prevail, indicated subsurface evaporation down to ∼3.5 m below land surface, and vertical and lateral preferential transport of water, following erratic preferential infiltration events. Chloride (Cl-) concentrations in the vadose zone pore water substantially increased with depth, evidence of deep subsurface evaporation and down flushing of concentrated solutions from the evaporation zones during preferential infiltration events. These observations led to development of a Desiccation-Crack-Induced Salinization (DCIS) conceptual model. DCIS suggests that thermally driven convective air flow in the desiccation cracks induces evaporation and salinization in relatively deep sections of the subsurface. This conceptual model supports previous conceptual models on vadose zone and groundwater salinization in fractured rock in arid environments and extends its validity to clayey soils in semi-arid environments.

  14. Characterization of the desiccation of wheat kernels by multivariate imaging.

    PubMed

    Jaillais, B; Perrin, E; Mangavel, C; Bertrand, D

    2011-06-01

    Variations in the quality of wheat kernels can be an important problem in the cereal industry. In particular, desiccation conditions play an essential role in both the technological characteristics of the kernel and its ability to sprout. In planta desiccation constitutes a key stage in the determinism of the functional properties of seeds. The impact of desiccation on the endosperm texture of seed is presented in this work. A simple imaging system had previously been developed to acquire multivariate images to characterize the heterogeneity of food materials. A special algorithm for the use under principal component analysis (PCA) was developed to process the acquired multivariate images. Wheat grains were collected at physiological maturity, and were subjected to two types of drying conditions that induced different kinetics of water loss. A data set containing 24 images (dimensioned 702 × 524 pixels) corresponding to the different desiccation stages of wheat kernels was acquired at different wavelengths and then analyzed. A comparison of the images of kernel sections highlighted changes in kernel texture as a function of their drying conditions. Slow drying led to a floury texture, whereas fast drying caused a glassy texture. The automated imaging system thus developed is sufficiently rapid and economical to enable the characterization in large collections of grain texture as a function of time and water content.

  15. Transcriptional Response of Saccharomyces cerevisiae to Desiccation and Rehydration†

    PubMed Central

    Singh, Jatinder; Kumar, Deept; Ramakrishnan, Naren; Singhal, Vibha; Jervis, Jody; Garst, James F.; Slaughter, Stephen M.; DeSantis, Andrea M.; Potts, Malcolm; Helm, Richard F.

    2005-01-01

    A transcriptional analysis of the response of Saccharomyces cerevisiae strain BY4743 to controlled air-drying (desiccation) and subsequent rehydration under minimal glucose conditions was performed. Expression of genes involved in fatty acid oxidation and the glyoxylate cycle was observed to increase during drying and remained in this state during the rehydration phase. When the BY4743 expression profile for the dried sample was compared to that of a commercially prepared dry active yeast, strikingly similar expression changes were observed. The fact that these two samples, dried by different means, possessed very similar transcriptional profiles supports the hypothesis that the response to desiccation is a coordinated event independent of the particular conditions involved in water removal. Similarities between “stationary-phase-essential genes” and those upregulated during desiccation were also noted, suggesting commonalities in different routes to reduced metabolic states. Trends in extracellular and intracellular glucose and trehalose levels suggested that the cells were in a “holding pattern” during the rehydration phase, a concept that was reinforced by cell cycle analyses. Application of a “redescription mining” algorithm suggested that sulfur metabolism is important for cell survival during desiccation and rehydration. PMID:16332871

  16. Desiccation tolerance of prokaryotes: application of principles to human cells.

    PubMed

    Potts, Malcolm; Slaughter, Stephen M; Hunneke, Frank-U; Garst, James F; Helm, Richard F

    2005-11-01

    The loss of water from cells is a stress that was likely imposed very early in evolution. An understanding of the sensitivity or tolerance of cells to depletion of intracellular water is relevant to the study of quiescence, longevity and aging, because one consequence of air-drying is full metabolic arrest, sometimes for extended periods. When considering the adaptation of cells to physiological extremes of pH, temperature or pressure, it is generally assumed that evolution is driven toward optimum function rather than maximum stability. However, adaptation to desiccation has the singular and crucial distinction that dried cells do not grow, and the time the cell is dried may represent the greater part of the life (the time the cell remains viable) of that cell and its component macromolecules. Is a consideration of "function" relevant in the context of desiccated cells? The response of prokaryotic cells to desiccation, and the mechanisms they employ to tolerate this stress at the level of the cell, genome and proteome are considered. Fundamental principles were then implemented in the design of strategies to achieve air-dry stabilization of sensitive eukaryotic (human) cells. The responses of the transcriptomes and proteomes of prokaryotic cells and eukaryotic cells (yeast and human) to drying in air are compared and contrasted to achieve an evolutionary context. The concept of the "desiccome" is developed to question whether there is common set of structural, physiological and molecular mechanisms that constitute desiccation tolerance. PMID:21676831

  17. [Desiccation tolerance in seeds of Prosopisferox and Pterogyne nitens (Fabaceae)].

    PubMed

    Morandini, Marcelo Nahuel; Giamminola, Eugenia Mabel; de Viana, Marta Leonor

    2013-03-01

    The high number of endemisms and species diversity together with the accelerated biodiversity loss by deforestation, especially in North Western Argentina, points out the need to work on species conservation combining ex situ and in situ strategies. The aim of this work was to study the desiccation tolerance in seeds of P ferox and P nitens for long term ex situ conservation at the Germplasm Bank of Native Species (BGEN) of the National University of Salta (Argentina). The fruits were collected from ten individuals in P ferox at the National Park Los Cardones and from two sites (Orán and Rivadavia) for P nitens. Desiccation tolerance was assessed following previous established methodologies. The moisture content (MC) of the seeds was determined by keeping them in oven at 103 degreeC and weighting the samples at different intervals till constant weight. Germination essays were carried out with two treatments (control and scarification), with different seed MC (fresh, 10-12%, 3-5%) and in desiccated seeds (3-5% MC) stored six months at -20 degreeC. The MC in P ferox seeds was 14.2% and 10% in P nitens, for both populations studied. Percentage germination in P ferox was higher in the scarification treatments (<82%). The difference between treatments increased with the reduction in MC and the storage for six months at -20 degreeC. Fresh seeds of P nitens do not need scarification treatment, but it is required with the reduction in MC and storage. Mean germination percentage of desiccated seeds stored six months at -20 degreeC was similar in both populations and greater than 82%.We concluded that both species are probably orthodox because seeds tolerated desiccation to 3-5% and storage for six months at -20 degree C. PMID:23894986

  18. Deep Vadose Zone Treatability Test for the Hanford Central Plateau: Interim Post-Desiccation Monitoring Results

    SciTech Connect

    Truex, Michael J.; Oostrom, Martinus; Strickland, Christopher E.; Johnson, Timothy C.; Johnson, Christian D.; Clayton, Ray E.; Chronister, Glen B.

    2013-09-01

    A field test of desiccation is being conducted as an element of the deep vadose zone treatability test program. Desiccation technology relies on removal of water from a portion of the subsurface such that the resultant low moisture conditions inhibit downward movement of water and dissolved contaminants. Previously, a field test report (Truex et al. 2012a) was prepared describing the active desiccation portion of the test and initial post-desiccation monitoring data. Additional monitoring data have been collected at the field test site during the post-desiccation period and is reported herein along with interpretation with respect to desiccation performance. This is an interim report including about 2 years of post-desiccation monitoring data.

  19. Alternatives to compressor cooling in California climates

    SciTech Connect

    Feustel, H. ); de Almeida, A. . Dept. of Electrical Engineering); Blumstein, C. . Universitywide Energy Research Group)

    1991-01-01

    This review and discussion has been prepared for the California Institute for Energy Efficiency (CIEE) to examine research on alternatives to compressor cooling. The report focuses on strategies for eliminating compressors in California's transition climates -- moderately warm areas located between the cool coastal regions and the hot central regions. Many of these strategies could also help reduce compressor use in hotter climates. Compressor-driven cooling of residences in California's transition climate regions is an undesirable load for California's electric utilities because load factor is poor and usage is typically high during periods of system peak demand. We review a number of alternatives to compressors, including low-energy strategies: evaporative cooling, natural and induced ventilation, reflective coatings, shading with vegetation and improved glazing, thermal storage, and radiative cooling. Also included are two energy-intensive strategies: absorption cooling and desiccant cooling. Our literature survey leads us to conclude that many of these strategies, used either singly or in combination, are technically and economically feasible alternatives to compressor-driven cooling. 78 refs., 8 figs.

  20. Deep Vadose Zone Treatability Test for the Hanford Central Plateau. Interim Post-Desiccation Monitoring Results, Fiscal Year 2015

    SciTech Connect

    Truex, Michael J.; Strickland, Christopher E.; Oostrom, Martinus; Johnson, Christian D.; Tartakovsky, Guzel D.; Johnson, Timothy C.; Clayton, Ray E.; Chronister, Glen B.

    2015-09-01

    A field test of desiccation is being conducted as an element of the Deep Vadose Zone Treatability Test Program. The active desiccation portion of the test has been completed. Monitoring data have been collected at the field test site during the post-desiccation period and are reported herein. This is an interim data summary report that includes about 4 years of post-desiccation monitoring data. The DOE field test plan proscribes a total of 5 years of post-desiccation monitoring.

  1. Desiccation stress at sub-zero temperatures in polar terrestrial arthropods.

    PubMed

    Worland, M Roger; Block, William

    2003-03-01

    Cold tolerant polar terrestrial arthropods have evolved a range of survival strategies which enable them to survive the most extreme environmental conditions (cold and drought) they are likely to encounter. Some species are classified as being freeze tolerant but the majority of those found in the Antarctic survive sub-zero temperatures by avoiding freezing by supercooling. For many arthropods, not just polar species, survival of desiccating conditions is equally important to survival of low temperatures. At sub-zero temperatures freeze avoiding arthropods are susceptible to desiccation and may lose water due to a vapour diffusion gradient between their supercooled body fluids and ice in their surroundings. This process ceases once the body fluids are frozen and so is not a problem for freeze tolerant species. This paper compares five polar arthropods, which have evolved different low temperature survival strategies, and the effects of exposure to sub-zero temperatures on their supercooling points (SCP) and water contents. The Antarctic oribatid mite (Alaskozetes antarcticus) reduced its supercooling point temperature from -6 to -30 degrees C, when exposed to decreasing sub-zero temperatures (cooled from 5 to -10 degrees C over 42 days) with little loss of body water during that period. However, Cryptopygus antarcticus, a springtail which occupies similar habitats in the Antarctic, showed a decrease in both water content and supercooling ability when exposed to the same experimental protocol. Both these Antarctic arthropods have evolved a freeze avoiding survival strategy. The Arctic springtail (Onychiurus arcticus), which is also freeze avoiding, dehydrated (from 2.4 to 0.7 g water g(-1) dry weight) at sub-zero temperatures and its SCP was lowered from c. -3 to below -15 degrees C in direct response to temperature (5 to -5.5 degrees C). In contrast, the freeze tolerant larvae of an Arctic fly (Heleomyza borealis) froze at c. -7 degrees C with little change in

  2. Clay with Desiccation Cracks is an Advection Dominated Environment

    NASA Astrophysics Data System (ADS)

    Baram, S.; Kurtzman, D.; Sher, Y.; Ronen, Z.; Dahan, O.

    2012-04-01

    Heavy clay sediments are regarded "safe" from the hydrological point of view due to their low hydraulic conductivities. However, the formation of desiccation cracks in dispersive clays may dramatically change their bulk hydraulic properties. The impact of desiccation cracks on water percolation, dissolved salts and contaminants transport and redox related reactions (microbial ammonium oxidation and denitrification) were investigated in 6 -12 m clay layer near a diary farm waste lagoon. The study implemented unique vadose-zone monitoring systems that enable in-situ measurements of the temporal variation of the sediment's water content along with frequent sampling of the sediment's pore water along the entire vadose zone (> 30 m). Results from four years of continuous measurements showed quick rises in sediment water content following rain events and temporal wastewater overflows. The percolation pattern indicated dominance of preferential flow through a desiccation-cracks network crossing the entire clay sediment layer. High water-propagation velocities (0.4 - 23.6 m h-1) were observed, indicating that the desiccation-crack network remains open and serves as a preferential flow pathway year-round, even at high sediment water content (~0.50 m3 m-3). The rapid percolation bypassed the most bio-geo-active parts of the soil, transporting even highly sorptive contaminants (testosterone and estrogen) in to the deep sections of the vadose zone, accelerating the underlying groundwater contamination. The ammonium and nitrate concentrations in the vadose zone and the high number of nitrifying and denitrifying bacteria (~108 gene copies gdry-sediemt-1, each) found in the sediment indicated that the entire vadose zone is aerated even at high water content conditions (~0.55 m3 m-3). The dissolved salts concentration in the pore-water and the δ2H-H2O and δ18O-H2O values of the pore-water substantially increased with depth (becoming less depleted) in the clay sediment

  3. Desiccation Tolerance in the Moss Polytrichum formosum: Physiological and Fine-structural Changes during Desiccation and Recovery

    PubMed Central

    Proctor, Michael C. F.; Ligrone, Roberto; Duckett, Jeffrey G.

    2007-01-01

    Background and Aims This study explores basic physiological features and time relations of recovery of photosynthetic activity and CO2 uptake following rehydration of a desiccation-tolerant moss in relation to the full temporal sequence of cytological changes associated with recovery to the normal hydrated state. It seeks reconciliation of the apparently conflicting published physiological and cytological evidence on recovery from desiccation in bryophytes. Methods Observations were made of water-stress responses and recovery using infrared gas analysis and modulated chlorophyll fluorescence, and of structural and ultrastructural changes by light and transmission electron microscopy. Key Results Net CO2 uptake fell to zero at approx. 40 % RWC, paralleling the fluorescence parameter ΦPSII at 200 µmol m–2 s–1 PPFD. On re-wetting the moss after 9–18 d desiccation, the initially negative net CO2 uptake became positive 10–30 min after re-wetting, restoring a net carbon balance after approx. 0·3–1 h. The parameter Fv/Fm reached approx. 80 % of its pre-desiccation value within approx. 10 min of re-wetting. In the presence of the protein-synthesis inhibitors chloramphenicol and cycloheximide, recovery of Fv/Fm (and CO2 exchange) proceeded normally in the dark, but declined rapidly in the light. Though initial recovery was rapid, both net CO2 uptake and Fv/Fm required approx. 24 h to recover completely to pre-desiccation values. The fixation protocols produced neither swelling of tissues nor plasmolysis. Thylakoids, grana and mitochondrial cristae remained intact throughout the drying–re-wetting cycle, but there were striking changes in the form of the organelles, especially the chloroplasts, which had prominent lobes and lamellar extensions in the normally hydrated state, but rounded off when desiccated, returning slowly to their normal state within approx. 24 h of re-wetting. Sub-cellular events during desiccation and re-wetting were generally

  4. Experimental heat and mass transfer of the separated and coupled rotating desiccant wheel and heat wheel

    SciTech Connect

    Enteria, Napoleon; Yoshino, Hiroshi; Mochida, Akashi; Takaki, Rie; Satake, Akira; Yoshie, Ryuichiro; Mitamura, Tiruaki; Baba, Seizo

    2010-07-15

    The experimental evaluation of the separated and coupled rotating desiccant wheel and heat wheel is reported. The study aims to investigate the performance of the desiccant wheel and of the heat wheel both when operated separately and jointly. The performance evaluation of the desiccant wheel is based on its moisture removal capacity (MRC), moisture removal regeneration (MRR), and moisture mass balance (MMB). In addition, the study used the total energy balance (TEB), sensible coefficient of performance (COP{sub Sensible}), latent coefficient of performance (COP{sub Latent}) and, total coefficient of performance (COP{sub Total}). The performance of the heat wheel is based on its effectiveness. The COP{sub Sensible}, COP{sub Latent} and, COP{sub Total} are used in the performance evaluation of the coupled desiccant wheel and heat wheel. The general results of the study show that the MRC, MRR and MMB coupled with the TEB, COP{sub Latent}, COP{sub Sensible} and COP{sub Total} predict adequately the performance of the desiccant wheel. In addition, the coupled operation of the desiccant wheel and heat wheel, contributed to the reduction of the external thermal energy requirement for the regeneration of the desiccant wheel. This study can be applied in other researches seeking evaluation of the desiccant wheel, heat wheel, and their combined operation. Moreover, the data presented here are significant for the desiccant wheel benchmarking and for evaluation of the desiccant wheel models. (author)

  5. STANDARDS CONTROLLING AIR EMISSIONS FOR THE SOIL DESICCATION PILOT TEST

    SciTech Connect

    BENECKE MW

    2010-09-08

    This air emissions document supports implementation of the Treatability Test Plan for Soil Desiccation as outlined in the Deep Vadose Zone Treatability Test Plan for the Hanford Central Plateau (DOE/RL-2007-56). Treatability testing supports evaluation of remedial technologies for technetium-99 (Tc-99) contamination in the vadose zone at sites such as the BC Cribs and Trenches. Soil desiccation has been selected as the first technology for testing because it has been recommended as a promising technology in previous Hanford Site technology evaluations and because testing of soil desiccation will provide useful information to enhance evaluation of other technologies, in particular gas-phase remediation technologies. A soil desiccation pilot test (SDPT) will evaluate the desiccation process (e.g., how the targeted interval is dried) and the long-term performance for mitigation of contaminant transport. The SDPT will dry out a moist zone contaminated by Tc-99 and nitrate that has been detected at Well 299-E13-62 (Borehole C5923). This air emissions document applies to the activities to be completed to conduct the SDPT in the 200-BC-1 operable unit located in the 200 East Area of the Hanford Site. Well 299-E13-62 is planned to be used as an injection well. This well is located between and approximately equidistant from cribs 216-B-16, 216-B-17, 216-B-18. and 216-B-19. Nitrogen gas will be pumped at approximately 300 ft{sup 3}/min into the 299-EI3-62 injection well, located approximately 12 m (39 ft) away from extraction well 299-EI3-65. The soil gas extraction rate will be approximately 150 ft{sup 3}/min. The SDPT will be conducted continuously over a period of approximately six months. The purpose of the test is to evaluate soil desiccation as a potential remedy for protecting groundwater. A conceptual depiction is provided in Figure 1. The soil desiccation process will physically dry, or evaporate, some of the water from the moist zone of interest. As such, it is

  6. Thrips domiciles protect larvae from desiccation in an arid environment.

    PubMed

    Gilbert, James D J

    2014-11-01

    Desiccation is a particular risk for small animals in arid environments. In response, many organisms "construct niches," favorable microenvironments where they spend part or all of their life cycle. Some maintain such environments for their offspring via parental care. Insect eggs are often protected from desiccation by parentally derived gels, casings, or cocoons, but active parental protection of offspring from desiccation has never been demonstrated. Most free-living thrips (Thysanoptera) alleviate water loss via thigmotaxis (crevice seeking). In arid Australia, Acacia thrips (Phlaeothripidae) construct many kinds of niche. Some thrips induce galls; others, like Dunatothrips aneurae, live and breed within "domiciles" made from loosely glued phyllodes. The function of domiciles is unknown; like other constructed niches, they may 1) create favorable microenvironments, 2) facilitate feeding, 3) protect from enemies, or a combination. To test the first 2 alternatives experimentally, field-collected domiciles were destroyed or left intact. Seven-day survival of feeding and nonfeeding larval stages was monitored at high (70-80%) or low (8-10%, approximately ambient) humidity. Regardless of humidity, most individuals survived in intact domiciles, whereas for destroyed domiciles, survival depended on humidity, suggesting parents construct and maintain domiciles to prevent offspring desiccating. Feeding and nonfeeding larvae had similar survival patterns, suggesting the domicile's role is not nutritional. Outside domiciles, survival at "high" humidity was intermediate, suggesting very high humidity requirements, or energetic costs of wandering outside domiciles. D. aneurae commonly cofound domiciles; cofoundresses may benefit both from shared nestbuilding costs, and from "deferred byproduct mutualism," that is, backup parental care in case of mortality.

  7. Development and Analysis of Desiccant Enhanced Evaporative Air Conditioner Prototype

    SciTech Connect

    Kozubal, E.; Woods, J.; Judkoff, R.

    2012-04-01

    This report documents the design of a desiccant enhanced evaporative air conditioner (DEVAP AC) prototype and the testing to prove its performance. Previous numerical modeling and building energy simulations indicate a DEVAP AC can save significant energy compared to a conventional vapor compression AC (Kozubal et al. 2011). The purposes of this research were to build DEVAP prototypes, test them to validate the numerical model, and identify potential commercialization barriers.

  8. Thrips domiciles protect larvae from desiccation in an arid environment

    PubMed Central

    2014-01-01

    Desiccation is a particular risk for small animals in arid environments. In response, many organisms “construct niches,” favorable microenvironments where they spend part or all of their life cycle. Some maintain such environments for their offspring via parental care. Insect eggs are often protected from desiccation by parentally derived gels, casings, or cocoons, but active parental protection of offspring from desiccation has never been demonstrated. Most free-living thrips (Thysanoptera) alleviate water loss via thigmotaxis (crevice seeking). In arid Australia, Acacia thrips (Phlaeothripidae) construct many kinds of niche. Some thrips induce galls; others, like Dunatothrips aneurae, live and breed within “domiciles” made from loosely glued phyllodes. The function of domiciles is unknown; like other constructed niches, they may 1) create favorable microenvironments, 2) facilitate feeding, 3) protect from enemies, or a combination. To test the first 2 alternatives experimentally, field-collected domiciles were destroyed or left intact. Seven-day survival of feeding and nonfeeding larval stages was monitored at high (70–80%) or low (8–10%, approximately ambient) humidity. Regardless of humidity, most individuals survived in intact domiciles, whereas for destroyed domiciles, survival depended on humidity, suggesting parents construct and maintain domiciles to prevent offspring desiccating. Feeding and nonfeeding larvae had similar survival patterns, suggesting the domicile’s role is not nutritional. Outside domiciles, survival at “high” humidity was intermediate, suggesting very high humidity requirements, or energetic costs of wandering outside domiciles. D. aneurae commonly cofound domiciles; cofoundresses may benefit both from shared nestbuilding costs, and from “deferred byproduct mutualism,” that is, backup parental care in case of mortality. PMID:25419084

  9. Reversible Inactivation and Desiccation Tolerance of Silicified Viruses

    SciTech Connect

    Laidler, James J.; Shugart, Jessica A.; Cady, Sherry L.; Bahjat, Keith S.; Stedman, Kenneth M.

    2013-11-19

    Long-distance host-independent virus dispersal is poorly understood, especially for viruses found in isolated ecosystems. To demonstrate a possible dispersal mechanism, we show that bacteriophage T4, archaeal virus SSV-K and Vaccinia are reversibly inactivated by mineralization in silica under conditions similar to volcanic hot springs. By contrast, bacteriophage PRD1 is not silicified. Moreover silicification provides viruses with remarkable desiccation resistance, which could allow extensive aerial dispersal.

  10. Colorado State University program for developing, testing, evaluation and optimizing solar heating and cooling systems. Project status report, August--September 1993

    SciTech Connect

    Not Available

    1993-09-01

    This report describes activities of the Colorado State University program on solar heating and cooling systems for the months of August and September 1993. The topics include: rating and certification of domestic water heating systems, unique solar system components, advanced residential solar domestic hot water systems, and desiccant cooling of buildings.

  11. Photosynthesis in desiccation tolerant plants: energy metabolism and antioxidative stress defense.

    PubMed

    Dinakar, Challabathula; Djilianov, Dimitar; Bartels, Dorothea

    2012-01-01

    Resurrection plants are regarded as excellent models to study the mechanisms associated with desiccation tolerance. During the past years tremendous progress has been made in understanding the phenomenon of desiccation tolerance in resurrection plants, but many questions are open concerning the mechanisms enabling these plants to survive desiccation. The photosynthetic apparatus is very sensitive to reactive oxygen species mediated injury during desiccation and must be maintained or quickly repaired upon rehydration. The photosynthetic apparatus is a primary source of generating reactive oxygen species. The unique ability of plants to withstand the oxidative stress imposed by reactive oxygen species during desiccation depends on the production of antioxidants. The present review considers the overall strategies and the mechanisms involved in the desiccation tolerance in the first part and will focus on the effects on photosynthesis, energy metabolism and antioxidative stress defenses in the second part.

  12. An Experimental Evolution Test of the Relationship between Melanism and Desiccation Survival in Insects

    PubMed Central

    Rajpurohit, Subhash; Peterson, Lisa Marie; Orr, Andrew J.; Marlon, Anthony J.; Gibbs, Allen G.

    2016-01-01

    We used experimental evolution to test the ‘melanism-desiccation’ hypothesis, which proposes that dark cuticle in several Drosophila species is an adaptation for increased desiccation tolerance. We selected for dark and light body pigmentation in replicated populations of D. melanogaster and assayed several traits related to water balance. We also scored pigmentation and desiccation tolerance in populations selected for desiccation survival. Populations in both selection regimes showed large differences in the traits directly under selection. However, after over 40 generations of pigmentation selection, dark-selected populations were not more desiccation-tolerant than light-selected and control populations, nor did we find significant changes in mass or carbohydrate amounts that could affect desiccation resistance. Body pigmentation of desiccation-selected populations did not differ from control populations after over 140 generations of selection, although selected populations lost water less rapidly. Our results do not support an important role for melanization in Drosophila water balance. PMID:27658246

  13. Cooling wall

    SciTech Connect

    Nosenko, V.I.

    1995-07-01

    Protecting the shells of blast furnaces is being resolved by installing cast iron cooling plates. The cooling plates become non-operational in three to five years. The problem is that defects occur in manufacturing the cooling plates. With increased volume and intensity of work placed on blast furnaces, heat on the cast iron cooling plates reduces their reliability that limits the interim repair period of blast furnaces. Scientists and engineers from the Ukraine studied this problem for several years, developing a new method of cooling the blast furnace shaft called the cooling wall. Traditional cast iron plates were replaced by a screen of steel tubes, with the area between the tubes filled with fireproof concrete. Before placing the newly developed furnace shaft into operation, considerable work was completed such as theoretical calculations, design, research of temperature fields and tension. Continual testing over many years confirms the value of this research in operating blast furnaces. The cooling wall works with water cooling as well as vapor cooling and is operating in 14 blast furnaces in the Ukraine and two in Russia, and has operated for as long as 14 years.

  14. Desiccation resistance and contamination as mechanisms of gaia.

    PubMed

    Brown, S; Margulis, L; Ibarra, S; Siqueiros, D

    1985-01-01

    The gaia hypothesis, formulated by J.E. Lovelock, asserts the composition of the reactive gases, the oxidation-reduction state and the temperature of the lower atmosphere of the planet Earth are actively regulated by the biota. Lovelock and Watson, using highly simplified mathematical models, have shown that the modulation of atmospheric temperature can be achieved by exponentially growing populations of differently colored organisms ("dark and light daisies"). It is more likely that the modulation of atmospheric gas composition is based on the colligative properties of exponentially growing mixed populations of microorganisms rather than on "daisies". Exponential growth of one population of microorganisms leads to gaseous and other metabolic products released to the environment, which favor the exponential growth of different populations, each with their own unique emissions. Extremely high densities of mixed populations of microorganisms ensue. These populations form structured microbial communities composed of members in varying states of activity. Growth potential of metabolically diverse populations most likely provides the basis for the responsiveness of the biota to changing environments. We have attempted to measure an aspect of the growth potential and diversity of one microbial community, that from a flat laminated microbial mat dominated by the cyanobacterium, Microcoleus. Microbial mat samples collected at yearly intervals between 1977 and 1982 were allowed to dry. Subsamples were revived under laboratory conditions by rewetting, and the resulting complex microbial populations were analyzed. Greater than 10(4) viable organisms per ml were estimated to be present in the desiccated samples. Only a portion of the diverse community could be characterized. There were at least 115 different types of desiccation resistant microorganisms present in these samples, primarily bacteria. However, more than a dozen types of rather uncommon fungi and protoctists were

  15. Desiccation resistance and contamination as mechanisms of gaia.

    PubMed

    Brown, S; Margulis, L; Ibarra, S; Siqueiros, D

    1985-01-01

    The gaia hypothesis, formulated by J.E. Lovelock, asserts the composition of the reactive gases, the oxidation-reduction state and the temperature of the lower atmosphere of the planet Earth are actively regulated by the biota. Lovelock and Watson, using highly simplified mathematical models, have shown that the modulation of atmospheric temperature can be achieved by exponentially growing populations of differently colored organisms ("dark and light daisies"). It is more likely that the modulation of atmospheric gas composition is based on the colligative properties of exponentially growing mixed populations of microorganisms rather than on "daisies". Exponential growth of one population of microorganisms leads to gaseous and other metabolic products released to the environment, which favor the exponential growth of different populations, each with their own unique emissions. Extremely high densities of mixed populations of microorganisms ensue. These populations form structured microbial communities composed of members in varying states of activity. Growth potential of metabolically diverse populations most likely provides the basis for the responsiveness of the biota to changing environments. We have attempted to measure an aspect of the growth potential and diversity of one microbial community, that from a flat laminated microbial mat dominated by the cyanobacterium, Microcoleus. Microbial mat samples collected at yearly intervals between 1977 and 1982 were allowed to dry. Subsamples were revived under laboratory conditions by rewetting, and the resulting complex microbial populations were analyzed. Greater than 10(4) viable organisms per ml were estimated to be present in the desiccated samples. Only a portion of the diverse community could be characterized. There were at least 115 different types of desiccation resistant microorganisms present in these samples, primarily bacteria. However, more than a dozen types of rather uncommon fungi and protoctists were

  16. Salinity effects on the dynamics and patterns of desiccation cracks

    NASA Astrophysics Data System (ADS)

    Shokri, N.; Zhou, P.

    2012-12-01

    Cracking arising from desiccation is a ubiquitous phenomenon encountered in various industrial and geo-environmental applications including drying of clayey soil, cement, ceramics, gels, and many more colloidal suspensions. Presence of cracks in muddy sediments modifies the characteristics of the medium such as pore structure, porosity, and permeability which in turn influence various flow and transport processes. Thus it remains a topic of great interest in many disciplines to describe the dynamics of desiccation cracking under various boundary conditions. To this end, we conducted a comprehensive study to investigate effects of NaCl concentrations on cracking dynamics and patterns during desiccation of Bentonite. Mixtures of Bentonite and NaCl solutions were prepared with NaCl concentration varying from 2 to 10 percent in 0.5 percent increment (totally 17 configurations). The slurry was placed in a Petri dish mounted on a digital balance to record the evaporation dynamics. The atmospheric conditions were kept constant using an environmental chamber. An automatic camera was used to record the dynamics of macro-cracks (mm scale) at the surface of desiccating clay each minute. The obtained results illustrate the significant effects of salt concentration on the initiation, propagation, morphology and general dynamics of macro-cracks. We found that higher salt concentrations results in larger macro cracks' lengths attributed to the effects of NaCl on compressing the electric double layer of particles at increasing electrolyte concentrations which reduce considerably the repulsive forces among the particles and causing instability of the slurry and flocculation of the colloidal particles. Rheological measurements by means of a stress controlled rheometer revealed that the yield stress of the slurry decreases as NaCl concentration increases which may indicate aggregation of larger units in the slurry as a result of flocculation causing larger cracks' lengths due to

  17. Method and composition for molding low density desiccant syntactic foam articles

    DOEpatents

    Lula, James W.; Schicker, James R.

    1984-01-01

    A method and a composition are provided for molding low density desiccant syntactic foam articles. A low density molded desiccant article may be made as a syntactic foam by blending a thermosetting resin, microspheres and molecular sieve desiccant powder, molding and curing. Such articles have densities of 0.2-0.9 g/cc, moisture capacities of 1-12% by weight, and can serve as light weight structural supports.

  18. Desiccation as a Long-Term Survival Mechanism for the Archaeon Methanosarcina barkeri

    PubMed Central

    Anderson, Kimberly L.; Apolinario, Ethel E.

    2012-01-01

    Viable methanogens have been detected in dry, aerobic environments such as dry reservoir sediment, dry rice paddies and aerobic desert soils, which suggests that methanogens have mechanisms for long-term survival in a desiccated state. In this study, we quantified the survival rates of the methanogenic archaeon Methanosarcina barkeri after desiccation under conditions equivalent to the driest environments on Earth and subsequent exposure to different stress factors. There was no significant loss of viability after desiccation for 28 days for cells grown with either hydrogen or the methylotrophic substrates, but recovery was affected by growth phase, with cells desiccated during the stationary phase of growth having a higher rate of recovery after desiccation. Synthesis of methanosarcinal extracellular polysaccharide (EPS) significantly increased the viability of desiccated cells under both anaerobic and aerobic conditions compared with that of non-EPS-synthesizing cells. Desiccated M. barkeri exposed to air at room temperature did not lose significant viability after 28 days, and exposure of M. barkeri to air after desiccation appeared to improve the recovery of viable cells compared with that of desiccated cells that were never exposed to air. Desiccated M. barkeri was more resistant to higher temperatures, and although resistance to oxidative conditions such as ozone and ionizing radiation was not as robust as in other desiccation-resistant microorganisms, the protection mechanisms are likely adequate to maintain cell viability during periodic exposure events. The results of this study demonstrate that after desiccation M. barkeri has the innate capability to survive extended periods of exposure to air and lethal temperatures. PMID:22194299

  19. Preparation of perlite-based magnesium perchlorate desiccant with colour indicator.

    PubMed

    Wu, L; He, H

    1994-05-01

    A new desiccant consisting of magnesium perchlorate, expanded perlite and metal chelate was prepared. The performance tests show that the desiccant is superior to magnesium perchlorate desiccant in dehydration efficiency, absorption capacity for water, flow resistance, color indicator and regeneration. It can reduce the amount of water in gases to approximately 0.7 ppm(v/v). Its applications in gas analysis and purification were investigated.

  20. Effect of nitrogen starvation on desiccation tolerance of Arctic Microcoleus strains (cyanobacteria)

    PubMed Central

    Tashyreva, Daria; Elster, Josef

    2015-01-01

    Although desiccation tolerance of Microcoleus species is a well-known phenomenon, there is very little information about their limits of desiccation tolerance in terms of cellular water content, the survival rate of their cells, and the environmental factors inducing their resistance to drying. We have discovered that three Microcoleus strains, isolated from terrestrial habitats of the High Arctic, survived extensive dehydration (to 0.23 g water g-1 dry mass), but did not tolerate complete desiccation (to 0.03 g water g-1 dry mass) regardless of pre-desiccation treatments. However, these treatments were critical for the survival of incomplete desiccation: cultures grown under optimal conditions failed to survive even incomplete desiccation; a low temperature enabled only 0–15% of cells to survive, while 39.8–65.9% of cells remained alive and intact after nitrogen starvation. Unlike Nostoc, which co-exists with Microcoleus in Arctic terrestrial habitats, Microcoleus strains are not truly anhydrobiotic and do not possess constitutive desiccation tolerance. Instead, it seems that the survival strategy of Microcoleus in periodically dry habitats involves avoidance of complete desiccation, but tolerance to milder desiccation stress, which is induced by suboptimal conditions (e.g., nitrogen starvation). PMID:25904909

  1. Cool Shelter

    ERIC Educational Resources Information Center

    Praeger, Charles E.

    2005-01-01

    Amid climbing energy costs and tightening budgets, administrators at school districts, colleges and universities are looking for all avenues of potential savings while promoting sustainable communities. Cool metal roofing can save schools money and promote sustainable design at the same time. Cool metal roofing keeps the sun's heat from collecting…

  2. 40 CFR Appendix A to Subpart F of... - List of Qualified Energy Conservation Measures, Qualified Renewable Generation, and Measures...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...-outs) • Air conditioner (central and room) upgrades/replacements • Heat pump (ground source, solar... use • Improved equipment controls • Solar assisted space conditioning (ventilation, air-conditioning, and desiccant cooling) • Passive solar designs • Air conditioner and heat pump clean and...

  3. Desiccation tolerance of iron bacteria biofilms on Mars regolith simulants

    NASA Astrophysics Data System (ADS)

    Feyh, Nina; Szewzyk, Ulrich

    2010-05-01

    Iron oxidizing bacteria play an important role in the geological redox cycling of iron on earth. The redox change between Fe(II) and Fe(III) can be used for biological energy production [1]. Therefore iron oxidation in the iron rich martian soils may be or may have been microbially mediated. The microbial conversion of iron is considered to be an ancient form of metabolism [2], so it might have evolved on Mars as well. However, to exist in recent martian soils, bacteria must be able to endure dry and cold conditions. Neutrophilic iron oxidizers can be found in various iron rich aquatic environments, where they lead to the precipitation of insoluble ferric hydroxides. Some of these environments fall temporarily dry, what could have led to an adaptation to desiccation by bacteria, existing there. One strategy of iron bacteria to endure drought stress might be the formation of biofilms by excreting Extracellular Polymeric Substances (EPS). The deposition of iron hydroxides could enable them to endure dry conditions as well. For our experiments, neutrophilic iron oxidizing bacteria have been isolated from a creek in Bad Salzhausen/Hesse and temporarily drying out pools in Tierra del Fuego. Strains from aquatic environments in the national park "Unteres Odertal" and from water wells in Berlin/Brandenburg are included in the tests as well. In desiccation experiments, the capability of iron bacteria to tolerate dry conditions are investigated. The aim of our first experiment is the adaptation to dry conditions. Biofilms of 15 strains are grown on ceramic beads in liquid medium containing complexed Fe(II), established biofilms contain Fe(III) precipitates. The cultures are desiccated in a sterile airflow until the weight of the cultures remained constant. After a desiccation period of 9 h up to 7 d, the beads are transferred to fresh liquid medium. Adapted strains are used in further desiccation experiments, where biofilms are grown on two martian regolith simulants. These

  4. Cooled railplug

    DOEpatents

    Weldon, William F.

    1996-01-01

    The railplug is a plasma ignitor capable of injecting a high energy plasma jet into a combustion chamber of an internal combustion engine or continuous combustion system. An improved railplug is provided which has dual coaxial chambers (either internal or external to the center electrode) that provide for forced convective cooling of the electrodes using the normal pressure changes occurring in an internal combustion engine. This convective cooling reduces the temperature of the hot spot associated with the plasma initiation point, particularly in coaxial railplug configurations, and extends the useful life of the railplug. The convective cooling technique may also be employed in a railplug having parallel dual rails using dual, coaxial chambers.

  5. Cool Vest

    NASA Technical Reports Server (NTRS)

    1982-01-01

    ILC, Dover Division's lightweight cooling garment, called Cool Vest was designed to eliminate the harmful effects of heat stress; increases tolerance time in hot environments by almost 300 percent. Made of urethane-coated nylon used in Apollo, it works to keep the body cool, circulating chilled water throughout the lining by means of a small battery-powered pump. A pocket houses the pump, battery and the coolant which can be ice or a frozen gel, a valve control allows temperature regulation. One version is self-contained and portable for unrestrained movement, another has an umbilical line attached to an external source of coolant, such as standard tap water, when extended mobility is not required. It is reported from customers that the Cool Vest pays for itself in increased productivity in very high temperatures.

  6. Cool School.

    ERIC Educational Resources Information Center

    Stephens, Suzanne

    1980-01-01

    The design for Floyd Elementary School in Miami (Florida) seeks to harness solar energy to provide at least 70 percent of the annual energy for cooling needs and 90 percent for hot water. (Author/MLF)

  7. Development of a desiccated cadaver delivery system to apply entomopathogenic nematodes for control of soil pests

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pentomopathogenic nematodes may be more capable of controlling soil pests when they are harbored by desiccated cadavers. A small-scale system was developed from a modified crop seed planter to effectively deliver desiccated nematode-infected cadavers into the soil. The system mainly consists of a me...

  8. Competitive assessment of desiccant solar/gas systems for single family residences

    NASA Astrophysics Data System (ADS)

    1982-01-01

    The solar/gas desiccant space conditioning system was compared with competing gas and electric technologies. Benefits and costs to the residential gas customer were evaluated, and practical recommendations regarding an appropriate R&D agenda to maximize the probability of successful development of an advanced desiccant system for that market were provided.

  9. Antioxidant defences in hydrated and desiccated states of the tardigrade Paramacrobiotus richtersi.

    PubMed

    Rizzo, Angela M; Negroni, Manuela; Altiero, Tiziana; Montorfano, Gigliola; Corsetto, Paola; Berselli, Patrizia; Berra, Bruno; Guidetti, Roberto; Rebecchi, Lorena

    2010-06-01

    Reactive oxygen species (ROS) are formed in all aerobic organisms, potentially leading to oxidative damage of all biological molecules. A number of defence mechanisms have developed to protect the organism from attack by ROS. Desiccation tolerance is correlated with an increase in the antioxidant potential in several organisms, but the regulation of the antioxidant defence system is complex and its role in desiccation-tolerant organisms is not yet firmly established. To determine if anhydrobiotic tardigrades have an antioxidant defence system, capable of counteracting ROS, we compared the activity of several antioxidant enzymes, the fatty acid composition and Heat shock protein expression in two physiological states (desiccated vs. hydrated) of the tardigrade Paramacrobiotus richtersi. In hydrated tardigrades, superoxide dismutase and catalase show comparable activities, while in desiccated specimens the activity of superoxide dismutase increases. Both glutathione peroxidase and glutathione were induced by desiccation. The percentage of fatty acid composition of polyunsaturated fatty acids and the amount of thiobarbituric acid reactive substances are higher in desiccated animals than in hydrated ones. Lastly, desiccated tardigrades did not differ significantly from the hydrated ones in the relative levels of Hsp70 and Hsp90. These results indicate that the possession of antioxidant metabolism could represent a crucial strategy to avoid damages during desiccation in anhydrobiotic tardigrades.

  10. Rapid desiccation hardening changes the cuticular hydrocarbon profile of Drosophila melanogaster.

    PubMed

    Stinziano, Joseph R; Sové, Richard J; Rundle, Howard D; Sinclair, Brent J

    2015-02-01

    The success of insects in terrestrial environments is due in large part to their ability to resist desiccation stress. Since the majority of water is lost across the cuticle, a relatively water-impermeable cuticle is a major component of insect desiccation resistance. Cuticular permeability is affected by the properties and mixing effects of component hydrocarbons, and changes in cuticular hydrocarbons can affect desiccation tolerance. A pre-exposure to a mild desiccation stress increases duration of desiccation survival in adult female Drosophila melanogaster, via a decrease in cuticular permeability. To test whether this acute response to desiccation stress is due to a change in cuticular hydrocarbons, we treated male and female D. melanogaster to a rapid desiccation hardening (RDH) treatment and used gas chromatography to examine the effects on cuticular hydrocarbon composition. RDH led to reduced proportions of unsaturated and methylated hydrocarbons compared to controls in females, but although RDH modified the cuticular hydrocarbon profile in males, there was no coordinated pattern. These data suggest that the phenomenon of RDH leading to reduced cuticular water loss occurs via an acute change in cuticular hydrocarbons that enhances desiccation tolerance in female, but not male, D. melanogaster.

  11. Extended periods of hydration do not elicit dehardening to desiccation tolerance in regeneration trials of the moss Syntrichia caninervis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Dehardening (deacclimation) to water stress is seldom studied in plants, and yet is an integral phase of desiccation tolerance. Most bryophytes are desiccation tolerant (DT), and yet even fully DT species lose a significant portion of their ability to withstand desiccation if dehardened. Shoots of t...

  12. Solar assisted alkali pretreatment of garden biomass: Effects on lignocellulose degradation, enzymatic hydrolysis, crystallinity and ultra-structural changes in lignocellulose.

    PubMed

    Gabhane, Jagdish; William, S P M Prince; Vaidya, Atul N; Das, Sera; Wate, Satish R

    2015-06-01

    A comprehensive study was carried out to assess the effectiveness of solar assisted alkali pretreatment (SAAP) on garden biomass (GB). The pretreatment efficiency was assessed based on lignocellulose degradation, conversion of cellulose into reducing sugars, changes in the ultra-structure and functional groups of lignocellulose and impact on the crystallinity of cellulose, etc. SAAP was found to be efficient for the removal of lignin and hemicellulose that facilitated enzymatic hydrolysis of cellulose. FTIR and XRD studies provided details on the effectiveness of SAAP on lignocellulosic moiety and crystallinity of cellulose. Scanning electron microscopic analysis showed ultra-structural disturbances in the microfibrils of GB as a result of pretreatment. The mass balance closer of 97.87% after pretreatment confirmed the reliability of SAAP pretreatment. Based on the results, it is concluded that SAAP is not only an efficient means of pretreatment but also economical as it involved no energy expenditure for heat generation during pretreatment.

  13. Colorado State University program for developing, testing, evaluating and optimizing solar heating and cooling systems

    SciTech Connect

    Not Available

    1992-03-23

    The objective is to develop and test various integrated solar heating, cooling and domestic hot water systems, and to evaluate their performance. Systems composed of new, as well as previously tested, components are carefully integrated so that effects of new components on system performance can be clearly delineated. The SEAL-DOE program includes six tasks which have received funding for the 1991--92 fifteen-month period. These include: (1) a project employing isothermal operation of air and liquid solar space heating systems, (2) a project to build and test several generic solar water heaters, (3) a project that will evaluate advanced solar domestic hot water components and concepts and integrate them into solar domestic hot water systems, (4) a liquid desiccant cooling system development project, (5) a project that will perform system modeling and analysis work on solid desiccant cooling systems research, and (6) a management task. The objectives and progress in each task are described in this report.

  14. Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons

    PubMed Central

    Arcaz, Arthur C.; Huestis, Diana L.; Dao, Adama; Yaro, Alpha S.; Diallo, Moussa; Andersen, John; Blomquist, Gary J.; Lehmann, Tovi

    2016-01-01

    ABSTRACT The African malaria mosquitoes Anopheles gambiae and Anopheles coluzzii range over forests and arid areas, where they withstand dry spells and months-long dry seasons, suggesting variation in their desiccation tolerance. We subjected a laboratory colony (G3) and wild Sahelian mosquitoes during the rainy and dry seasons to desiccation assays. The thoracic spiracles and amount and composition of cuticular hydrocarbons (CHCs) of individual mosquitoes were measured to determine the effects of these traits on desiccation tolerance. The relative humidity of the assay, body water available, rate of water loss and water content at death accounted for 88% of the variation in desiccation tolerance. Spiracle size did not affect the rate of water loss or desiccation tolerance of the colony mosquitoes, as was the case for the total CHCs. However, six CHCs accounted for 71% of the variation in desiccation tolerance and three accounted for 72% of the variation in the rate of water loss. Wild A. coluzzii exhibited elevated desiccation tolerance during the dry season. During that time, relative thorax and spiracle sizes were smaller than during the rainy season. A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant. Seasonal changes in CHC composition were detected in Sahelian A. coluzzii. Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance. In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism. PMID:27207644

  15. Colorado State University program for developing, testing, evaluating and optimizing solar heating and cooling systems. Project status report, March--November 1992

    SciTech Connect

    Not Available

    1993-01-07

    This program includes six tasks, including (1) a project measuring the performance of unique solar system components, (2) a project to develop a methodology for determining annual performance ratings of solar domestic hot water systems, (3) a project that will identify, analyze, design, build, and experimentally evaluate SDHW systems incorporating advanced concepts and components, (4) a liquid desiccant cooling system development project, (5) a project that will perform TRNSYS simulations to determine potential energy savings for desiccant cooling systems, especially in humid climates, and (6) a management task. The objectives and progress in each task are described.

  16. Cooling Vest

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Because quadriplegics are unable to perspire below the level of spinal injury, they cannot tolerate heat stress. A cooling vest developed by Ames Research Center and Upjohn Company allows them to participate in outdoor activities. The vest is an adaptation of Ames technology for thermal control garments used to remove excess body heat of astronauts. The vest consists of a series of corrugated channels through which cooled water circulates. Its two outer layers are urethane coated nylon, and there is an inner layer which incorporates the corrugated channels. It can be worn as a backpack or affixed to a wheelchair. The unit includes a rechargeable battery, mini-pump, two quart reservoir and heat sink to cool the water.

  17. Cooled railplug

    DOEpatents

    Weldon, W.F.

    1996-05-07

    The railplug is a plasma ignitor capable of injecting a high energy plasma jet into a combustion chamber of an internal combustion engine or continuous combustion system. An improved railplug is provided which has dual coaxial chambers (either internal or external to the center electrode) that provide for forced convective cooling of the electrodes using the normal pressure changes occurring in an internal combustion engine. This convective cooling reduces the temperature of the hot spot associated with the plasma initiation point, particularly in coaxial railplug configurations, and extends the useful life of the railplug. The convective cooling technique may also be employed in a railplug having parallel dual rails using dual, coaxial chambers. 10 figs.

  18. Method and apparatus for extracting water from air using a desiccant

    DOEpatents

    Spletzer, Barry L.; Callow, Diane Schafer

    2003-01-01

    The present invention provides a method and apparatus for extracting liquid water from moist air using minimal energy input. The method can be considered as four phases: (1) adsorbing water from air into a desiccant, (2) isolating the water-laden desiccant from the air source, (3) desorbing water as vapor from the desiccant into a chamber, and (4) isolating the desiccant from the chamber, and compressing the vapor in the chamber to form liquid condensate. The liquid condensate can be removed for use. Careful design of the dead volumes and pressure balances can minimize the energy required. The dried air can be exchanged for fresh moist air and the process repeated. An apparatus comprises a first chamber in fluid communication with a desiccant, and having ports to intake moist air and exhaust dried air. The apparatus also comprises a second chamber in fluid communication with the desiccant. The second chamber allows variable internal pressure, and has a port for removal of liquid condensate. Each chamber can be configured to be isolated or in communication with the desiccant. The first chamber can be configured to be isolated or in communication with a course of moist air. Various arrangements of valves, pistons, and chambers are described.

  19. Properties of a new liquid desiccant solution - Lithium chloride and calcium chloride mixture

    SciTech Connect

    Ertas, A.; Anderson, E.E.; Kiris, I. )

    1992-09-01

    Desiccants, broadly classified as solid and liquid desiccants, have the property of extracting and retaining moisture from air brought into contact with them. By using either type, moisture in the air is removed and the resulting dry air can be used for air-conditioning or drying purposes. Because of its properties, lithium chloride is the most stable liquid desiccant and has a large dehydration concentration (30% to 45%), but its cost is relatively high ($9.00-13.00 per kg). It is expected that lithium chloride will reduce the relative humidity to as low as 15%. Calcium chloride is the cheapest (45 cents per kg) and most readily available desiccant, but it has the disadvantage of being unstable depending on the air inlet conditions and the concentration of the desiccant in the solution. To stabilize calcium chloride and to decrease the high cost of lithium chloride, the two can be mixed in different weight combinations. The main objective of this research is to measure the physical properties of different combinations of this mixture such as density, viscosity, and vapor pressure which are necessary for analysis of heat and mass transfer in a packed tower desiccant-air contact system. The solubility of this new liquid desiccant under certain temperature-concentrations will also be studied.

  20. Effects of periodic desiccation on the synthesis of the UV-screening compound, scytonemin, in cyanobacteria.

    PubMed

    Fleming, Erich D; Castenholz, Richard W

    2007-06-01

    Scytonemin is an ultraviolet radiation (UVR)-screening compound synthesized by some sheathed cyanobacteria exposed to high solar and sky radiation. It is primarily produced in response to UVA radiation, but certain environmental stresses can enhance synthesis. This study focuses on the effects of periodic desiccation on scytonemin synthesis in three desiccation-tolerant cyanobacterial strains, Nostoc punctiforme PCC 73102, Chroococcidiopsis CCMEE 5056 and Chroococcidiopsis CCMEE 246. Nostoc punctiforme and Chroococcidiopsis CCMEE 5056 exposed to UVA radiation produced more concentrated scytonemin screens when experiencing periodic desiccation (i.e. 1 day desiccated for every 2 days hydrated) than when continuously hydrated. A more concentrated scytonemin screen would reduce the amount of UVR damage accrued when cells are desiccated and metabolically inactive. This might allow the cyanobacteria to allocate more energy to systems other than UVR damage repair during rehydration, which would facilitate recovery. The scytonemin screen is extremely stable, remaining largely intact in the sheaths of desiccated N. punctiforme even when continuously exposed to UVA radiation for about 2 months. In contrast to the above findings, scytonemin synthesis in Chroococcidiopsis CCMEE 246, a strain that produces scytonemin constitutively under low visible light (no UVA), was partially inhibited by periodic desiccation.

  1. Desiccation tolerance in resurrection plants: new insights from transcriptome, proteome and metabolome analysis

    PubMed Central

    Dinakar, Challabathula; Bartels, Dorothea

    2013-01-01

    Most higher plants are unable to survive desiccation to an air-dried state. An exception is a small group of vascular angiosperm plants, termed resurrection plants. They have evolved unique mechanisms of desiccation tolerance and thus can tolerate severe water loss, and mostly adjust their water content with the relative humidity in the environment. Desiccation tolerance is a complex phenomenon and depends on the regulated expression of numerous genes during dehydration and subsequent rehydration. Most of the resurrection plants have a large genome and are difficult to transform which makes them unsuitable for genetic approaches. However, technical advances have made it possible to analyze changes in gene expression on a large-scale. These approaches together with comparative studies with non-desiccation tolerant plants provide novel insights into the molecular processes required for desiccation tolerance and will shed light on identification of orphan genes with unknown functions. Here, we review large-scale recent transcriptomic, proteomic, and metabolomic studies that have been performed in desiccation tolerant plants and discuss how these studies contribute to understanding the molecular basis of desiccation tolerance. PMID:24348488

  2. Identification of proteins involved in desiccation tolerance in the red seaweed Pyropia orbicularis (Rhodophyta, Bangiales).

    PubMed

    López-Cristoffanini, Camilo; Zapata, Javier; Gaillard, Fanny; Potin, Philippe; Correa, Juan A; Contreras-Porcia, Loretto

    2015-12-01

    Extreme reduction in cellular water content leads to desiccation, which, if persistent, affects the physiology of organisms, mainly through oxidative stress. Some organisms are highly tolerant to desiccation, including resurrection plants and certain intertidal seaweeds. One such species is Pyropia orbicularis, a rhodophycean that colonizes upper intertidal zones along the Chilean coast. Despite long, daily periods of air exposure due to tides, this alga is highly tolerant to desiccation. The present study examined the proteome of P. orbicularis by 2DE and LC-MS/MS analyses to determine the proteins associated with desiccation tolerance (DT). The results showed that, under natural conditions, there were significant changes in the protein profile during low tide as compared to naturally hydrated plants at high tide. These changes were mainly in newly appeared proteins spots such as chaperones, monodehydroascorbate reductase, and manganese superoxide dismutase, among others. Previously undescribed proteins under desiccation conditions included phycobiliproteins, glyoxalase I, and phosphomannomutase. These changes evidenced that several physiological responses involved in DT are activated during low tide, including decreased photosynthetic activity, increased antioxidant capacity, and the preservation of cell physiology by regulating water content, cell wall structure, and cell volume. Similar responses have been observed in resurrection plants and bryophytes exposed to desiccation. Therefore, the coordinated activation of different desiccation tolerance pathways in P. orbicularis could explain the successful biological performance of this seaweed in the upper intertidal rocky zones.

  3. Field-Scale Assessment of Desiccation Implementation for Deep Vadose Zone Contaminants

    SciTech Connect

    Truex, Michael J.; Oostrom, Martinus; Strickland, Christopher E.; Chronister, Glen B.; Benecke, Mark W.; Johnson, Christian D.

    2012-11-01

    Desiccation of the vadose zone has the potential to reduce the flux of contaminants to underlying groundwater by removing moisture and decreasing the aqueous-phase permeability of the desiccated zone. However, data to evaluate implementation of desiccation are needed to enable consideration of desiccation as a potential remedy. Implementation of desiccation was field tested by injecting dry nitrogen gas to a target treatment zone and monitoring the spatial and temporal progress of the drying process. Aqueous waste discharges to disposal cribs approximately 50 years ago distributed water and contaminants, including primarily technetium-99 and nitrate, within the 100-m deep vadose zone at the test site. A field test location was selected adjacent to one of the former disposal cribs. The test was conducted in a contaminated portion of the vadose zone dominated by fine sands with lenses of silt material. Desiccation reduced volumetric moisture content to as low as 0.01. The lateral and vertical distribution of drying from the injection well was influenced by the subsurface heterogeneity. However, over time, desiccation occurred in the initially wetter, lower permeability lenses.

  4. Relaxation nuclear magnetic resonance imaging (R-NMRI) of desiccation in M9787 silicone pads.

    SciTech Connect

    Alam, Todd M; Cherry, Brian Ray; Alam, Mary Kathleen

    2004-06-01

    The production and aging of silicone materials remains an important issue in the weapons stockpile due to their utilization in a wide variety of components and systems within the stockpile. Changes in the physical characteristics of silicone materials due to long term desiccation has been identified as one of the major aging effects observed in silicone pad components. Here we report relaxation nuclear magnetic resonance imaging (R-NMRI) spectroscopy characterization of the silica-filled and unfilled polydimethylsiloxane (PDMS) and polydiphenylsiloxane (PDPS) copolymer (M9787) silicone pads within desiccating environments. These studies were directed at providing additional details about the heterogeneity of the desiccation process. Uniform NMR spin-spin relaxation time (T2) images were observed across the pad thickness indicating that the drying process is approximately uniform, and that the desiccation of the M9787 silicone pad is not a H2O diffusion limited process. In a P2O5 desiccation environment, significant reduction of T2 was observed for the silica-filled and unfilled M9787 silicone pad for desiccation up to 225 days. A very small reduction in T2 was observed for the unfilled copolymer between 225 and 487 days. The increase in relative stiffness with desiccation was found to be higher for the unfilled copolymer. These R-NMRI results are correlated to local changes in the modulus of the material

  5. Aquatic insects dealing with dehydration: do desiccation resistance traits differ in species with contrasting habitat preferences?

    PubMed Central

    Velasco, Josefa; Millán, Andrés; Bilton, David T.; Arribas, Paula

    2016-01-01

    Background Desiccation resistance shapes the distribution of terrestrial insects at multiple spatial scales. However, responses to drying stress have been poorly studied in aquatic groups, despite their potential role in constraining their distribution and diversification, particularly in arid and semi-arid regions. Methods We examined desiccation resistance in adults of four congeneric water beetle species (Enochrus, family Hydrophilidae) with contrasting habitat specificity (lentic vs. lotic systems and different salinity optima from fresh- to hypersaline waters). We measured survival, recovery capacity and key traits related to desiccation resistance (fresh mass, % water content, % cuticle content and water loss rate) under controlled exposure to desiccation, and explored their variability within and between species. Results Meso- and hypersaline species were more resistant to desiccation than freshwater and hyposaline ones, showing significantly lower water loss rates and higher water content. No clear patterns in desiccation resistance traits were observed between lotic and lentic species. Intraspecifically, water loss rate was positively related to specimens’ initial % water content, but not to fresh mass or % cuticle content, suggesting that the dynamic mechanism controlling water loss is mainly regulated by the amount of body water available. Discussion Our results support previous hypotheses suggesting that the evolution of desiccation resistance is associated with the colonization of saline habitats by aquatic beetles. The interespecific patterns observed in Enochrus also suggest that freshwater species may be more vulnerable than saline ones to drought intensification expected under climate change in semi-arid regions such as the Mediterranean Basin. PMID:27635346

  6. Crucial role of extracellular polysaccharides in desiccation and freezing tolerance in the terrestrial cyanobacterium Nostoc commune.

    PubMed

    Tamaru, Yoshiyuki; Takani, Yayoi; Yoshida, Takayuki; Sakamoto, Toshio

    2005-11-01

    The cyanobacterium Nostoc commune is adapted to the terrestrial environment and has a cosmopolitan distribution. In this study, the role of extracellular polysaccharides (EPS) in the desiccation tolerance of photosynthesis in N. commune was examined. Although photosynthetic O2 evolution was not detected in desiccated colonies, the ability of the cells to evolve O2 rapidly recovered after rehydration. The air-dried colonies contained approximately 10% (wt/wt) water, and field-isolated, natural colonies with EPS were highly water absorbent and were rapidly hydrated by atmospheric moisture. The cells embedded in EPS in Nostoc colonies were highly desiccation tolerant, and O2 evolution was not damaged by air drying. Although N. commune was determined to be a mesophilic cyanobacterium, the cells with EPS were heat tolerant in a desiccated state. EPS could be removed from cells by homogenizing colonies with a blender and filtering with coarse filter paper. This treatment to remove EPS did not damage Nostoc cells or their ability to evolve O2, but O2 evolution was significantly damaged by desiccation treatment of the EPS-depleted cells. Similar to the EPS-depleted cells, the laboratory culture strain KU002 had only small amount of EPS and was highly sensitive to desiccation. In the EPS-depleted cells, O2 evolution was also sensitive to freeze-thaw treatment. These results strongly suggest that EPS of N. commune is crucial for the stress tolerance of photosynthesis during desiccation and during freezing and thawing.

  7. Aquatic insects dealing with dehydration: do desiccation resistance traits differ in species with contrasting habitat preferences?

    PubMed Central

    Velasco, Josefa; Millán, Andrés; Bilton, David T.; Arribas, Paula

    2016-01-01

    Background Desiccation resistance shapes the distribution of terrestrial insects at multiple spatial scales. However, responses to drying stress have been poorly studied in aquatic groups, despite their potential role in constraining their distribution and diversification, particularly in arid and semi-arid regions. Methods We examined desiccation resistance in adults of four congeneric water beetle species (Enochrus, family Hydrophilidae) with contrasting habitat specificity (lentic vs. lotic systems and different salinity optima from fresh- to hypersaline waters). We measured survival, recovery capacity and key traits related to desiccation resistance (fresh mass, % water content, % cuticle content and water loss rate) under controlled exposure to desiccation, and explored their variability within and between species. Results Meso- and hypersaline species were more resistant to desiccation than freshwater and hyposaline ones, showing significantly lower water loss rates and higher water content. No clear patterns in desiccation resistance traits were observed between lotic and lentic species. Intraspecifically, water loss rate was positively related to specimens’ initial % water content, but not to fresh mass or % cuticle content, suggesting that the dynamic mechanism controlling water loss is mainly regulated by the amount of body water available. Discussion Our results support previous hypotheses suggesting that the evolution of desiccation resistance is associated with the colonization of saline habitats by aquatic beetles. The interespecific patterns observed in Enochrus also suggest that freshwater species may be more vulnerable than saline ones to drought intensification expected under climate change in semi-arid regions such as the Mediterranean Basin.

  8. Identification of proteins involved in desiccation tolerance in the red seaweed Pyropia orbicularis (Rhodophyta, Bangiales).

    PubMed

    López-Cristoffanini, Camilo; Zapata, Javier; Gaillard, Fanny; Potin, Philippe; Correa, Juan A; Contreras-Porcia, Loretto

    2015-12-01

    Extreme reduction in cellular water content leads to desiccation, which, if persistent, affects the physiology of organisms, mainly through oxidative stress. Some organisms are highly tolerant to desiccation, including resurrection plants and certain intertidal seaweeds. One such species is Pyropia orbicularis, a rhodophycean that colonizes upper intertidal zones along the Chilean coast. Despite long, daily periods of air exposure due to tides, this alga is highly tolerant to desiccation. The present study examined the proteome of P. orbicularis by 2DE and LC-MS/MS analyses to determine the proteins associated with desiccation tolerance (DT). The results showed that, under natural conditions, there were significant changes in the protein profile during low tide as compared to naturally hydrated plants at high tide. These changes were mainly in newly appeared proteins spots such as chaperones, monodehydroascorbate reductase, and manganese superoxide dismutase, among others. Previously undescribed proteins under desiccation conditions included phycobiliproteins, glyoxalase I, and phosphomannomutase. These changes evidenced that several physiological responses involved in DT are activated during low tide, including decreased photosynthetic activity, increased antioxidant capacity, and the preservation of cell physiology by regulating water content, cell wall structure, and cell volume. Similar responses have been observed in resurrection plants and bryophytes exposed to desiccation. Therefore, the coordinated activation of different desiccation tolerance pathways in P. orbicularis could explain the successful biological performance of this seaweed in the upper intertidal rocky zones. PMID:26154304

  9. Effect of desiccation and resubmersion on the oxidative stress response of the kuruma shrimp Marsupenaeus japonicus.

    PubMed

    Duan, Yafei; Zhang, Jiasong; Dong, Hongbiao; Wang, Yun; Liu, Qingsong; Li, Hua

    2016-02-01

    In the present study, the oxidative stress response in hepatopancreas of Marsupenaeus japonicus to desiccation stress and resubmersed in seawater were studied, such as respiratory burst, ROS production ( [Formula: see text] ), activities of antioxidant enzymes (CAT, GPx, SOD, POD and GST) and oxidative damage to lipid and protein (indexed by contents of MDA). The duration of desiccation significantly influenced shrimp survival, and the mortality rates were 37.5% and 87.5% after desiccation 5 h and 10 h, respectively. After desiccation stress 3 h, the respiratory burst, ROS production, and the activity of SOD and CAT were up-regulated significantly. The activity of GPx and POD, and the content of MDA decreased significantly at 0.5 h and 1 h, and then increased significantly at 3 h. But GST activity was no significant change after desiccation. During the resubmersion period, most of the antioxidant enzymes activities could recover to the control level at 24 h, but a small quantity of the oxidative stress still existed in tissues. HE staining showed that desiccation stress induced damage symptoms in hepatopancreas of M. japonicus. These results revealed that desiccation influenced the antioxidative status and caused oxidative stress and tissue damage via confusion of antioxidant enzymes in M. japonicus, but the oxidative stress could be eliminated within a certain range after the shrimps were resubmersed in seawater.

  10. Field Demonstration of Active Desiccant Modules Designed to Integrate with Standard Unitary Rooftop Package Equipment - Final Report: Phase 3

    SciTech Connect

    Fischer, J

    2004-03-15

    overall electrical energy consumption and a 12.5-kW reduction in peak demand. The cost of gas used for regeneration of the desiccant wheel over this period of time is estimated to be only $740, using a gas cost of $0.50 per therm--the summer rate in 2001. The estimated net savings is $5400 annually, resulting in a 1-2 year payback. It is likely that similar energy/cost savings were realized at the Callaway Gardens hotel. In this installation, however, a central plant supplied the chilled water serving fan coil units in the hotel wing retrofitted with the ADM, so it was not metered separately. Consequently, the owner could not provide actual energy consumption data specific to the facility. The energy and operating cost savings at both sites are directly attributable to higher cooling-season thermostat settings and decreased conventional system run times. These field installations were selected as an immediate and appropriate response to correct indoor humidity and fresh air ventilation problems being experienced by building occupants and owners, so no rigorous baseline-building vs. test-building energy use/operating cost savings results can be presented. The report presents several simulated comparisons between the ADM/roof HVAC approach and other equipment combinations, where both desiccant and conventional systems are modeled to provide comparable fresh air ventilation rates and indoor humidity levels. The results obtained from these simulations demonstrate convincingly the energy and operating cost savings obtainable with this hybrid desiccant/vapor-compression technology, verifying those actually seen at the pilot installations. The ADM approach is less expensive than conventional alternatives providing similar performance and indoor air quality and provides a very favorable payback (1 year or so) compared with oversized rooftop units that cannot be operated effectively with the necessary high outdoor air percentages.

  11. Comparison of ground-coupled solar-heat-pump systems to conventional systems for residential heating, cooling and water heating

    NASA Astrophysics Data System (ADS)

    Choi, M. K.; Morehouse, J. H.; Hughes, P. J.

    1981-07-01

    An analysis is performed of ground-coupled stand-alone and series configured solar-assisted liquid-to-air heat pump systems for residences. The year-round thermal performance of these systems for space heating, space cooling, and water heating is determined by simulation and compared against non-ground-coupled solar heat pump systems as well as conventional heating and cooling systems in three geographic locations: Washington, DC; Fort Worth, Texas; and Madison, Wisconsin. The results indicate that without tax credits a combined solar/ground-coupled heat pump system for space heating and cooling is not cost competitive with conventional systems. Its thermal performance is considerably better than non-ground-coupled solar heat pumps in Fort Worth. Though the ground-coupled stand-alone heat pump provides 51 percent of the heating and cooling load with non-purchased energy in Fort Worth, its thermal performance in Washington and Madison is poor.

  12. Experimental and numerical investigation on the performance of an internally cooled dehumidifier

    NASA Astrophysics Data System (ADS)

    Turgut, Oguz Emrah; Çoban, Mustafa Turhan

    2016-02-01

    Liquid desiccant based dehumidifiers are important components of the air conditioning applications. Internally cooled dehumidifiers with liquid desiccants are deemed to be superior to the adiabatic types, thanks to the cooling medium which takes away the latent heat of vaporization occured when moist air contacts with liquid desiccant. However, its utilization in industrial applications is restricted due to the inherent corrosive characteristics of the liquid desiccants. In this study, an experimental chamber is built for epoxy coated plate fin type dehumidifier which is used in order to diminish the corrosive effect of the lithium chloride aqueous solution. Dehumidification effectiveness and moisture removal rate, two parameter indices, are adopted to measure the performance of the air conditioning system. The effect of inlet operating parameters on moisture removal rates is extensively analyzed. Two dimensional numerical model adapted from the conservation principles is utilized for obtainment of output parameters. Experimental results are compared with the numerical model and comparisons show that numerical outputs agrees with the experimental results. And also, dehumidification performance of lithium chloride and lithium bromide aqueous solutions are evaluated and compared against each other.

  13. Gene Transfer to the Desiccation-Tolerant Cyanobacterium Chroococcidiopsis

    PubMed Central

    Billi, Daniela; Friedmann, E. Imre; Helm, Richard F.; Potts, Malcolm

    2001-01-01

    The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe the first system for genetic manipulation of Chroococcidiopsis. Plasmids pDUCA7 and pRL489, based on the pDU1 replicon of Nostoc sp. strain PCC 7524, were transferred to different isolates of Chroococcidiopsis via conjugation and electroporation. This report provides the first evidence that pDU1 replicons can be maintained in cyanobacteria other than Nostoc and Anabaena. Following conjugation, both plasmids replicated in Chroococcidiopsis sp. strains 029, 057, and 123 but not in strains 171 and 584. Both plasmids were electroporated into strains 029 and 123 but not into strains 057, 171, and 584. Expression of PpsbA-luxAB on pRL489 was visualized through in vivo luminescence. Efficiencies of conjugative transfer for pDUCA7 and pRL489 into Chroococcidiopsis sp. strain 029 were approximately 10−2 and 10−4 transconjugants per recipient cell, respectively. Conjugative transfer occurred with a lower efficiency into strains 057 and 123. Electrotransformation efficiencies of about 10−4 electrotransformants per recipient cell were achieved with strains 029 and 123, using either pDUCA7 or pRL489. Extracellular deoxyribonucleases were associated with each of the five strains. Phylogenetic analysis, based upon the V6 to V8 variable regions of 16S rRNA, suggests that desert strains 057, 123, 171, and 029 are distinct from the type species strain Chroococcidiopsis thermalis PCC 7203. The high efficiency of conjugative transfer of Chroococcidiopsis sp. strain 029, from the Negev Desert, Israel, makes this a suitable experimental strain for genetic studies on desiccation tolerance. PMID:11244070

  14. The precipitation response to the desiccation of Lake Chad

    SciTech Connect

    Lauwaet D.; VanWeverberg K.; vanLipzig, N. P. M., Weverberg, K. V., Ridderb, K. D., and Goyens, C.

    2012-04-01

    Located in the semi-arid African Sahel, Lake Chad has shrunk from a surface area of 25000 km2 in 1960 to about 1350 km2 due to a series of droughts and anthropogenic influences. The disappearance of such a large open-water body can be expected to have a noticeable effect on the meteorology in the surroundings of the lake. The impact could extend even further to the west as westward propagating convective systems pass Lake Chad in the rainfall season. This study examines the sensitivity of the regional hydrology and convective processes to the desiccation of the lake using a regional atmospheric model. Three Lake Chad scenarios are applied reflecting the situation in 1960, the current situation and a potential future scenario in which the lake and the surrounding wetlands have disappeared. The model simulations span the months July-September in 2006, which includes the rainfall season in the Lake Chad area. Total precipitation amounts and the components of the hydrological cycle are found to be hardly affected by the existence of the lake. A filled Lake Chad does, however, increase the precipitation at the east side of the lake. The model results indicate that the boundary layer moisture and temperature are significantly altered downwind of the lake. By investigating a mesoscale convective system (MCS) case, this is found to affect the development and progress of the system. At first, the MCS is intensified by the more unstable boundary layer air but the persistence of the system is altered as the cold pool propagation becomes less effective. The proposed mechanism is able to explain the differences in the rainfall patterns nearby Lake Chad between the scenarios. This highlights the local sensitivity to the desiccation of Lake Chad whereas the large-scale atmospheric processes are not affected.

  15. Tolerance to oxidative stress induced by desiccation in Porphyra columbina (Bangiales, Rhodophyta)

    PubMed Central

    Contreras-Porcia, Loretto; Thomas, Daniela; Flores, Verónica; Correa, Juan A.

    2011-01-01

    Unravelling the mechanisms underlying desiccation tolerance is crucial in order to understand the position of algal species in the intertidal zone. The alga Porphyra columbina lives in the uppermost part of the rocky intertidal zones around the world and was selected as a model for this study. Naturally desiccated plants were collected during low tide and studied for morphological changes, oxidative burst induction, biomolecule oxidation, antioxidant responses, and photosynthetic status. Naturally hydrated plants collected during high tides were used for comparative purposes. In addition, changes induced by desiccation were assessed in vitro and the capacity to recover from desiccation was determined by rehydrating the fronds in seawater. The global results show that desiccation induces morphological and cellular alterations accompanied by a loss of ∼96% of the water content. Overproduction of reactive oxygen species (ROS) was induced by desiccation and two peaks of H2O2 were detected at 1 and 3 h of desiccation. However, during in vitro rehydration post-desiccation, the ROS quickly returned to the basal levels. At the biomolecular level, only a low production of oxidized proteins was recorded during desiccation, whereas the activity of diverse antioxidant enzymes increased. However, this activity diminished to near basal levels during rehydration. The photosynthetic efficiency (Fv/Fm) during desiccation declined by 94–96% of the values recorded in hydrated plants. This reduction was generated by the low levels of trapped energy flux per cross-section (TRo/CS), electron transport flux per CS (ETo/CS), and density of reaction centres (RC/SCo) as well as the chlorophyll content. The inverse pattern was observed for the levels of phycocyanin and phycoerythrin content. Fv/Fm and the photosynthetic indicators were restored to normal levels after only 5 min of rehydration. The results indicate that desiccation in P. columbina causes overproduction of ROS that is

  16. Parametric analysis of variables that affect the performance of a desiccant dehumidification system

    SciTech Connect

    Vineyard, E.A.; Sand, J.R.; Durfee, D.J.

    2000-07-01

    Desiccant dehumidification systems, which are used to reduce the moisture (latent load) of the conditioned air in buildings, are typically specified on the basis of grain depression (pounds of water removed per hour) for a given volumetric flow rate of air at a specified dry-bulb or wet-bulb temperature. While grain depression gives some indication of the performance of the system, it does not adequately describe the efficiency of the moisture removal process. Several operating parameters, such as desiccant wheel speed, regeneration temperature, volumetric air flow rate, wheel thickness, sector angle, and desiccant loading, affect the ability of the desiccant dehumidification system to remove moisture. There are so many design parameters that influence the operation of a desiccant system that it is difficult to quantify the impact from the interactions on system performance. The purpose of this study is to investigate the impact of varying some of these operating parameters on the performance of a desiccant dehumidification system and to report the results using more quantitative measures, such as latent capacity and latent coefficient of performance (COP), that better describe the efficiency of the moisture removal process. The results will be used to improve the understanding of the operation of desiccant systems and to optimize their performance by changing certain operating parameters or improving components. Two desiccant loadings were tested: one at normal production level and the other with 25% more desiccant applied to the wheel. For both desiccant loadings, the latent capacity and COP increased as desiccant wheel speed increased. As expected, latent capacity improved significantly as air flow rates increased. It is noted, however, that the efficiency (latent COP) was quite sensitive to air flow rate and showed a maximum at a particular flow rate that best matched the other operating/design conditions of the system. Finally, higher regeneration temperatures

  17. Laboratory and Modeling Evaluations in Support of Field Testing for Desiccation at the Hanford Site

    SciTech Connect

    Truex, Michael J.; Oostrom, Martinus; Freedman, Vicky L.; Strickland, Christopher E.; Wietsma, Thomas W.; Tartakovsky, Guzel D.; Ward, Anderson L.

    2011-02-23

    The Deep Vadose Zone Treatability Test Plan for the Hanford Central Plateau includes testing of the desiccation technology as a potential technology to be used in conjunction with surface infiltration control to limit the flux of technetium and other contaminants in the vadose zone to the groundwater. Laboratory and modeling efforts were conducted to investigate technical uncertainties related to the desiccation process and its impact on contaminant transport. This information is intended to support planning, operation, and interpretation of a field test for desiccation in the Hanford Central Plateau.

  18. The response of foodborne pathogens to osmotic and desiccation stresses in the food chain.

    PubMed

    Burgess, Catherine M; Gianotti, Andrea; Gruzdev, Nadia; Holah, John; Knøchel, Susanne; Lehner, Angelika; Margas, Edyta; Esser, Stephan Schmitz; Sela Saldinger, Shlomo; Tresse, Odile

    2016-03-16

    In combination with other strategies, hyperosmolarity and desiccation are frequently used by the food processing industry as a means to prevent bacterial proliferation, and particularly that of foodborne pathogens, in food products. However, it is increasingly observed that bacteria, including human pathogens, encode mechanisms to survive and withstand these stresses. This review provides an overview of the mechanisms employed by Salmonella spp., Shiga toxin producing E. coli, Cronobacter spp., Listeria monocytogenes and Campylobacter spp. to tolerate osmotic and desiccation stresses and identifies gaps in knowledge which need to be addressed to ensure the safety of low water activity and desiccated food products.

  19. Cool Sportswear

    NASA Technical Reports Server (NTRS)

    1982-01-01

    New athletic wear design based on the circulating liquid cooling system used in the astronaut's space suits, allows athletes to perform more strenuous activity without becoming overheated. Techni-Clothes gear incorporates packets containing a heat-absorbing gel that slips into an insulated pocket of the athletic garment and is positioned near parts of the body where heat transfer is most efficient. A gel packet is good for about one hour. Easily replaced from a supply of spares in an insulated container worn on the belt. The products, targeted primarily for runners and joggers and any other athlete whose performance may be affected by hot weather, include cooling headbands, wrist bands and running shorts with gel-pack pockets.

  20. Global cooling?

    PubMed

    Damon, P E; Kunen, S M

    1976-08-01

    The world's inhabitants, including Scientists, live primarily in the Northern Hemisphere. It is quite natural to be concerned about events that occur close to home and neglect faraway events. Hence, it is not surprising that so little attention has been given to the Southern Hemisphere. Evidence for global cooling has been based, in large part, on a severe cooling trend at high northern latitudes. This article points out that the Northern Hemisphere cooling trend appears to be out of phase with a warming trend at high latitudes in the Southern Hemisphere. The data are scanty. We cannot be sure that these temperature fluctuations are be not the result of natural causes. How it seems most likely that human activity has already significantly perturbed the atmospheric weather system. The effect of particulate matter pollution should be most severe in the highly populated and industrialized Northern Hemisphere. Because of the rapid diffusion of CO(2) molecules within the atmosphere, both hemispheres will be subject to warming due to the atmospheric (greenhouse) effect as the CO(2) content of the atmosphere builds up from the combustion of fossil fuels. Because of the differential effects of the two major sources of atmospheric pollution, the CO(2) greenhouse effect warming trend should first become evident in the Southern Hemisphere. The socioeconomic and political consequences of climate change are profound. We need an early warning system such as would be provided by a more intensive international world weather watch, particularly at high northern and southern latitudes.

  1. cis-3-Chloroacrylic Acid: A New Cotton Defoliant and Crop Desiccant.

    PubMed

    Herrett, R A; Kurtz, A N

    1963-09-20

    cis-3-Chloroacrylic acid is a potent cotton defoliant and a crop desiccant. Relationships between structure and activity indicate a relatively high degree of specificity, since minor modifications in structure result in loss of activity. PMID:17751800

  2. An effective desiccant system to regulate the humidity inside the chambers of the solid dosage forms.

    PubMed

    Lehto, Vesa-Pekka; Erling, Ida

    2007-11-01

    The most common way to protect moisture-sensitive pharmaceutical powders is to utilize protective packaging. However, the most convenient package materials are all permeable to water molecules to some extent and limited protection is normally achieved with this arrangement even though desiccants are employed. In the present study we introduce a novel system that can regulate the internal humidity of the containers used with solid dosage forms for a desired time at a requested level. Instead of the widely used solid adsorbents the system utilizes saturated salt solutions loaded in desiccant bags made of various polymer materials with appropriate permeation properties. By utilizing salt solutions the size of the desiccant bag can be further reduced. A wide variety of commonly used powder chambers and desiccant bags are tested, proving the effectiveness of the introduced system.

  3. The glyoxylate shunt is essential for desiccation tolerance in C. elegans and budding yeast

    PubMed Central

    Erkut, Cihan; Gade, Vamshidhar R; Laxman, Sunil; Kurzchalia, Teymuras V

    2016-01-01

    Many organisms, including species from all kingdoms of life, can survive desiccation by entering a state with no detectable metabolism. To survive, C. elegans dauer larvae and stationary phase S. cerevisiae require elevated amounts of the disaccharide trehalose. We found that dauer larvae and stationary phase yeast switched into a gluconeogenic mode in which metabolism was reoriented toward production of sugars from non-carbohydrate sources. This mode depended on full activity of the glyoxylate shunt (GS), which enables synthesis of trehalose from acetate. The GS was especially critical during preparation of worms for harsh desiccation (preconditioning) and during the entry of yeast into stationary phase. Loss of the GS dramatically decreased desiccation tolerance in both organisms. Our results reveal a novel physiological role for the GS and elucidate a conserved metabolic rewiring that confers desiccation tolerance on organisms as diverse as worm and yeast. DOI: http://dx.doi.org/10.7554/eLife.13614.001 PMID:27090086

  4. Development of desiccant based air conditioning for hotels and motels. Final report, phase 1

    SciTech Connect

    Banks, N.J.

    1994-10-01

    This report contains final reports from two phases of field tests: Phase I at the Marriott Courtyard in West Palm Beach, completed in October 1991, and Phase II at the Walt Disney World Swan Hotel in Orlando, completed in August 1993. The goal was to measure the performance and reliability of the desiccant units. The successfull program proved the value of the gas-fired desiccant technology to the hospitality industry. The desiccant units reduced moisture by 15 to 20% relative humidity. Measurements of temperature, humidity, and wallboard moisture content showed a dramatic reduction in the humidity levels experienced prior to installation of the units and in the control areas without desiccants. Moisture damage was kept in check and remodelling due to it was eliminated.

  5. cis-3-Chloroacrylic Acid: A New Cotton Defoliant and Crop Desiccant.

    PubMed

    Herrett, R A; Kurtz, A N

    1963-09-20

    cis-3-Chloroacrylic acid is a potent cotton defoliant and a crop desiccant. Relationships between structure and activity indicate a relatively high degree of specificity, since minor modifications in structure result in loss of activity.

  6. Efficiency of cold hardiness induction by desiccation stress in four winter cereals.

    PubMed

    Cloutier, Y; Andrews, C J

    1984-11-01

    A number of defined desiccation treatments without low temperature exposure were able to induce freezing tolerance in 20 cultivars of winter cereals. A maximal degree of freezing tolerance was induced in epicotyls at 24 degrees C in 24 hours at 40% relative humidity in rye and wheat, 7 days at 54% RH in barley, and 4 days at 70% RH in oats. Freezing tolerance was not correlated to water content of the plants after desiccation treatment but was related to the genetic capacity of the cultivars to frost harden. Levels of freezing tolerance induced by desiccation were similar to those induced by cold acclimation in rye and wheat, but considerably less in barley and oats. This is associated with a more rapid desiccation injury in barley and oats, precluding the completion of the hardening process. PMID:16663889

  7. Responses of the Lichen Photobiont Trebouxia erici to Desiccation and Rehydration (II) Proteomics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Lichen desiccation tolerance is associated with cellular protection mechanisms directed against the oxidative stress produced during dehydration and/or rehydration, however, these mechanisms are not well understood. In other poikilohydric organisms, changes in the synthesis of proteins have bee...

  8. Inorganic fluoride uptake as a measure of relative compatibility of molecular sieve desiccants with fluorocarbon refrigerants

    SciTech Connect

    Cohen, A.P.; Blackwell, C.S.

    1995-12-31

    The fluoride content of molecular sieve desiccants after exposure to R-32 in compatibility tests indicates the extent of the reaction of refrigerant with desiccant. The objective is to determine this fluoride content in a way that reports fluorine that has reacted with the desiccant, not fluorine that is present as adsorbed refrigerant. A conditioning procedure is described to remove adsorbed refrigerant by displacement with water vapor. The efficacy of this procedure is substantiated by {sup 19}F NMR spectroscopy. The conditioned desiccant undergoes pyrohydrolysis at a high temperature (975 C, 1787 F) to remove reacted fluorine as HF. Fluoride is determined in the resulting condensate using an ion-selective electrode. The ability of this technique to report accurate fluoride values is confirmed with standard reference materials.

  9. Experimental and Numerical Investigations of Soil Desiccation for Vadose Zone Remediation: Report for Fiscal Year 2007

    SciTech Connect

    Ward, Andy L.; Oostrom, Mart; Bacon, Diana H.

    2008-02-04

    Apart from source excavation, the options available for the remediation of vadose zone metal and radionuclide contaminants beyond the practical excavation depth (0 to 15 m) are quite limited. Of the available technologies, very few are applicable to the deep vadose zone with the top-ranked candidate being soil desiccation. An expert panel review of the work on infiltration control and supplemental technologies identified a number of knowledge gaps that would need to be overcome before soil desiccation could be deployed. The report documents some of the research conducted in the last year to fill these knowledge gaps. This work included 1) performing intermediate-scale laboratory flow cell experiments to demonstrate the desiccation process, 2) implementing a scalable version of Subsurface Transport Over Multiple Phases–Water-Air-Energy (STOMP-WAE), and 3) performing numerical experiments to identify the factors controlling the performance of a desiccation system.

  10. Theoretical study of the effect of liquid desiccant mass flow rate on the performance of a cross flow parallel-plate liquid desiccant-air dehumidifier

    NASA Astrophysics Data System (ADS)

    Mohammad, Abdulrahman Th.; Mat, Sohif Bin; Sulaiman, M. Y.; Sopian, K.; Al-abidi, Abduljalil A.

    2013-11-01

    A computer simulation using MATLAB is investigated to predict the distribution of air stream parameters (humidity ratio and temperature) as well as desiccant parameters (temperature and concentration) inside the parallel plate absorber. The present absorber consists of fourteen parallel plates with a surface area per unit volume ratio of 80 m2/m3. Calcium chloride as a liquid desiccant flows through the top of the plates to the bottom while the air flows through the gap between the plates making it a cross flow configuration. The model results show the effect of desiccant mass flow rate on the performance of the dehumidifier (moisture removal and dehumidifier effectiveness). Performance comparisons between present cross-flow dehumidifier and another experimental cross-flow dehumidifier in the literature are carried out. The simulation is expected to help in optimizing of a cross flow dehumidifier.

  11. Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags

    PubMed Central

    Adhikari, Bishwo N; Wall, Diana H; Adams, Byron J

    2009-01-01

    Background Nematodes are the dominant soil animals in Antarctic Dry Valleys and are capable of surviving desiccation and freezing in an anhydrobiotic state. Genes induced by desiccation stress have been successfully enumerated in nematodes; however we have little knowledge of gene regulation by Antarctic nematodes which can survive multiple environmental stresses. To address this problem we investigated the genetic responses of a nematode species, Plectus murrayi, that is capable of tolerating Antarctic environmental extremes, in particular desiccation and freezing. In this study, we provide the first insight into the desiccation induced transcriptome of an Antarctic nematode through cDNA library construction and suppressive subtractive hybridization. Results We obtained 2,486 expressed sequence tags (ESTs) from 2,586 clones derived from the cDNA library of desiccated P. murrayi. The 2,486 ESTs formed 1,387 putative unique transcripts of which 523 (38%) had matches in the model-nematode Caenorhabditis elegans, 107 (7%) in nematodes other than C. elegans, 153 (11%) in non-nematode organisms and 605 (44%) had no significant match to any sequences in the current databases. The 1,387 unique transcripts were functionally classified by using Gene Ontology (GO) hierarchy and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The results indicate that the transcriptome contains a group of transcripts from diverse functional areas. The subtractive library of desiccated nematodes showed 80 transcripts differentially expressed during desiccation stress, of which 28% were metabolism related, 19% were involved in environmental information processing, 28% involved in genetic information processing and 21% were novel transcripts. Expression profiling of 14 selected genes by quantitative Real-time PCR showed 9 genes significantly up-regulated, 3 down-regulated and 2 continuously expressed in response to desiccation. Conclusion The establishment of a desiccation EST

  12. Global changes in DNA methylation in seeds and seedlings of Pyrus communis after seed desiccation and storage.

    PubMed

    Michalak, Marcin; Barciszewska, Mirosława Z; Barciszewski, Jan; Plitta, Beata P; Chmielarz, Paweł

    2013-01-01

    The effects of storage and deep desiccation on structural changes of DNA in orthodox seeds are poorly characterized. In this study we analyzed the 5-methylcytosine (m(5)C) global content of DNA isolated from seeds of common pear (Pyrus communis L.) that had been subjected to extreme desiccation, and the seedlings derived from these seeds. Germination and seedling emergence tests were applied to determine seed viability after their desiccation. In parallel, analysis of the global content of m(5)C in dried seeds and DNA of seedlings obtained from such seeds was performed with a 2D TLC method. Desiccation of fresh seeds to 5.3% moisture content (mc) resulted in a slight reduction of DNA methylation, whereas severe desiccation down to 2-3% mc increased DNA methylation. Strong desiccation of seeds resulted in the subsequent generation of seedlings of shorter height. A 1-year period of seed storage induced a significant increase in the level of DNA methylation in seeds. It is possible that alterations in the m(5)C content of DNA in strongly desiccated pear seeds reflect a reaction of desiccation-tolerant (orthodox) seeds to severe desiccation. Epigenetic changes were observed not only in severely desiccated seeds but also in 3-month old seedlings obtained from these seeds. With regard to seed storage practices, epigenetic assessment could be used by gene banks for early detection of structural changes in the DNA of stored seeds.

  13. Global Changes in DNA Methylation in Seeds and Seedlings of Pyrus communis after Seed Desiccation and Storage

    PubMed Central

    Michalak, Marcin; Barciszewska, Mirosława Z.; Barciszewski, Jan; Plitta, Beata P.; Chmielarz, Paweł

    2013-01-01

    The effects of storage and deep desiccation on structural changes of DNA in orthodox seeds are poorly characterized. In this study we analyzed the 5-methylcytosine (m5C) global content of DNA isolated from seeds of common pear (Pyrus communis L.) that had been subjected to extreme desiccation, and the seedlings derived from these seeds. Germination and seedling emergence tests were applied to determine seed viability after their desiccation. In parallel, analysis of the global content of m5C in dried seeds and DNA of seedlings obtained from such seeds was performed with a 2D TLC method. Desiccation of fresh seeds to 5.3% moisture content (mc) resulted in a slight reduction of DNA methylation, whereas severe desiccation down to 2–3% mc increased DNA methylation. Strong desiccation of seeds resulted in the subsequent generation of seedlings of shorter height. A 1-year period of seed storage induced a significant increase in the level of DNA methylation in seeds. It is possible that alterations in the m5C content of DNA in strongly desiccated pear seeds reflect a reaction of desiccation-tolerant (orthodox) seeds to severe desiccation. Epigenetic changes were observed not only in severely desiccated seeds but also in 3-month old seedlings obtained from these seeds. With regard to seed storage practices, epigenetic assessment could be used by gene banks for early detection of structural changes in the DNA of stored seeds. PMID:23940629

  14. Thermal Inactivation of Desiccation-Adapted Salmonella spp. in Aged Chicken Litter

    PubMed Central

    Chen, Zhao; Diao, Junshu; Dharmasena, Muthu; Ionita, Claudia; Rieck, James

    2013-01-01

    Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with that in a nonadapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20, 30, 40, and 50% moisture contents for a 24-h desiccation adaptation. Afterwards, the compost with desiccation-adapted cells was inoculated into the aged chicken litter with the same moisture content for heat treatments at 70, 75, 80, 85, and 150°C. Recovery media were used to allow heat-injured cells to resuscitate. A 5-log reduction in the number of the desiccation-adapted cells in aged chicken litter with a 20% moisture content required >6, >6, ∼4 to 5, and ∼3 to 4 h of exposure at 70, 75, 80, and 85°C, respectively. As a comparison, a 5-log reduction in the number of nonadapted control cells in the same chicken litter was achieved within ∼1.5 to 2, ∼1 to 1.5, ∼0.5 to 1, and <0.5 h at 70, 75, 80, and 85°C, respectively. The exposure time required to obtain a 5-log reduction in the number of desiccation-adapted cells gradually became shorter as temperature and moisture content were increased. At 150°C, desiccation-adapted Salmonella cells survived for 50 min in chicken litter with a 20% moisture content, whereas control cells were detectable by enrichment for only 10 min. Our results demonstrated that the thermal resistance of Salmonella in aged chicken litter was increased significantly when the cells were adapted to desiccation. This study also validated the effectiveness of thermal processing being used for producing chicken litter free of Salmonella contamination. PMID:24014540

  15. Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter.

    PubMed

    Chen, Zhao; Diao, Junshu; Dharmasena, Muthu; Ionita, Claudia; Jiang, Xiuping; Rieck, James

    2013-11-01

    Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with that in a nonadapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20, 30, 40, and 50% moisture contents for a 24-h desiccation adaptation. Afterwards, the compost with desiccation-adapted cells was inoculated into the aged chicken litter with the same moisture content for heat treatments at 70, 75, 80, 85, and 150°C. Recovery media were used to allow heat-injured cells to resuscitate. A 5-log reduction in the number of the desiccation-adapted cells in aged chicken litter with a 20% moisture content required >6, >6, ∼4 to 5, and ∼3 to 4 h of exposure at 70, 75, 80, and 85°C, respectively. As a comparison, a 5-log reduction in the number of nonadapted control cells in the same chicken litter was achieved within ∼1.5 to 2, ∼1 to 1.5, ∼0.5 to 1, and <0.5 h at 70, 75, 80, and 85°C, respectively. The exposure time required to obtain a 5-log reduction in the number of desiccation-adapted cells gradually became shorter as temperature and moisture content were increased. At 150°C, desiccation-adapted Salmonella cells survived for 50 min in chicken litter with a 20% moisture content, whereas control cells were detectable by enrichment for only 10 min. Our results demonstrated that the thermal resistance of Salmonella in aged chicken litter was increased significantly when the cells were adapted to desiccation. This study also validated the effectiveness of thermal processing being used for producing chicken litter free of Salmonella contamination.

  16. Molecular Strategies of the Caenorhabditis elegans Dauer Larva to Survive Extreme Desiccation

    PubMed Central

    Erkut, Cihan; Vasilj, Andrej; Boland, Sebastian; Habermann, Bianca; Shevchenko, Andrej; Kurzchalia, Teymuras V.

    2013-01-01

    Massive water loss is a serious challenge for terrestrial animals, which usually has fatal consequences. However, some organisms have developed means to survive this stress by entering an ametabolic state called anhydrobiosis. The molecular and cellular mechanisms underlying this phenomenon are poorly understood. We recently showed that Caenorhabditis elegans dauer larva, an arrested stage specialized for survival in adverse conditions, is resistant to severe desiccation. However, this requires a preconditioning step at a mild desiccative environment to prepare the organism for harsher desiccation conditions. A systems approach was used to identify factors that are activated during this preconditioning. Using microarray analysis, proteomics, and bioinformatics, genes, proteins, and biochemical pathways that are upregulated during this process were identified. These pathways were validated via reverse genetics by testing the desiccation tolerances of mutants. These data show that the desiccation response is activated by hygrosensation (sensing the desiccative environment) via head neurons. This leads to elimination of reactive oxygen species and xenobiotics, expression of heat shock and intrinsically disordered proteins, polyamine utilization, and induction of fatty acid desaturation pathway. Remarkably, this response is specific and involves a small number of functional pathways, which represent the generic toolkit for anhydrobiosis in plants and animals. PMID:24324795

  17. Shoot desiccation and hydraulic failure in temperate woody angiosperms during an extreme summer drought.

    PubMed

    Nardini, Andrea; Battistuzzo, Marta; Savi, Tadeja

    2013-10-01

    Plant water status and hydraulics were measured in six woody angiosperms growing in a karstic woodland, during an extreme summer drought. Our aim was to take advantage of an unusual climatic event to identify key traits related to species-specific drought damage. The damage suffered by different species was assessed in terms of percentage of individuals showing extensive crown desiccation. Stem water potential (Ψstem ) and percent loss of hydraulic conductivity (PLC) were measured in healthy and desiccated individuals. Vulnerability to cavitation was assessed in terms of stem water potential inducing 50% PLC (Ψ50 ). Stem density (ρstem ) was also measured. Species-specific percentage of desiccated individuals was correlated to Ψ50 and ρstem . Crown desiccation was more widespread in species with less negative Ψ50 and lower ρstem . Desiccated individuals had lower Ψstem and higher PLC than healthy ones, suggesting that hydraulic failure was an important mechanism driving shoot dieback. Drought-vulnerable species showed lower safety margins (Ψstem  - Ψ50 ) than resistant ones. The Ψ50 , safety margins and ρstem values emerge as convenient traits to be used for tentative predictions of differential species-specific impact of extreme drought events on a local scale. The possibility that carbohydrate depletion was also involved in induction of desiccation symptoms is discussed.

  18. Method and composition for molding low-density desiccant syntactic-foam articles

    DOEpatents

    Not Available

    1981-12-07

    These and other objects of the invention are achieved by a process for molding to size a desiccant syntactic foam article having a density of 0.2 to 0.9 g/cc and a moisture capacity of 1 to 12% by weight, comprising the steps of: charging a mold with a powdery mixture of an activated desiccant, microspheres and a thermosetting resin, the amount of the desiccant being sufficient to provide the required moisture capacity, and the amounts of the microspheres and resin being such that the microspheres/desiccant volume fraction exceeds the packing factor by an amount sufficient to substantially avoid shrinkage without causing excessively high molding pressures; covering the mold and heating the covered mold to a temperature and for an amount of time sufficient to melt the resin; and tightly closing the mold and heating the closed mold to a temperature and for an amount of time sufficient to cure the resin, and removing the resultant desiccant syntactic foam article from the mold. In a composition of matter aspect, the present invention provides desiccant syntactic foam articles, and a composition of matter for use in molding the same.

  19. A desiccant/steam-injected gas-turbine industrial cogeneration system

    SciTech Connect

    Jody, B.J.; Daniels, E.J.; Karvelas, D.E.; Teotia, A.P.S.

    1993-01-01

    An integrated desiccant/steam-injected gas-turbine system was evaluated as an industrial cogenerator for the production of electricity and dry, heated air for product drying applications. The desiccant can be regenerated using the heated, compressed air leaving the compressor. The wet stream leaves the regenerator at a lower temperature than when it entered the desiccant regenerator, but with little loss of energy. The wet stream returns to the combustion chamber of the gas-turbine system after preheating by exchanging heat with the turbine exhaust strewn. Therefore, the desiccant is regenerated virtually energy-free. In the proposed system, the moisture-laden air exiting the desiccant is introduced into the combustion chamber of the gas-turbine power system. This paper discusses various possible design configurations, the impact of increased moisture content on the combustion process, the pressure drop across the desiccant regenerator, and the impact of these factors on the overall performance of the integrated system. A preliminary economic analysis including estimated potential energy savings when the system is used in several drying applications, and equipment and operating costs are also presented.

  20. A desiccant/steam-injected gas-turbine industrial cogeneration system

    SciTech Connect

    Jody, B.J.; Daniels, E.J.; Karvelas, D.E.; Teotia, A.P.S.

    1993-12-31

    An integrated desiccant/steam-injected gas-turbine system was evaluated as an industrial cogenerator for the production of electricity and dry, heated air for product drying applications. The desiccant can be regenerated using the heated, compressed air leaving the compressor. The wet stream leaves the regenerator at a lower temperature than when it entered the desiccant regenerator, but with little loss of energy. The wet stream returns to the combustion chamber of the gas-turbine system after preheating by exchanging heat with the turbine exhaust strewn. Therefore, the desiccant is regenerated virtually energy-free. In the proposed system, the moisture-laden air exiting the desiccant is introduced into the combustion chamber of the gas-turbine power system. This paper discusses various possible design configurations, the impact of increased moisture content on the combustion process, the pressure drop across the desiccant regenerator, and the impact of these factors on the overall performance of the integrated system. A preliminary economic analysis including estimated potential energy savings when the system is used in several drying applications, and equipment and operating costs are also presented.

  1. Response of the Leaf Cell Wall to Desiccation in the Resurrection Plant Myrothamnus flabellifolius1

    PubMed Central

    Moore, John P.; Nguema-Ona, Eric; Chevalier, Laurence; Lindsey, George G.; Brandt, Wolf F.; Lerouge, Patrice; Farrant, Jill M.; Driouich, Azeddine

    2006-01-01

    The Myrothamnus flabellifolius leaf cell wall and its response to desiccation were investigated using electron microscopic, biochemical, and immunocytochemical techniques. Electron microscopy revealed desiccation-induced cell wall folding in the majority of mesophyll and epidermal cells. Thick-walled vascular tissue and sclerenchymous ribs did not fold and supported the surrounding tissue, thereby limiting the extent of leaf shrinkage and allowing leaf morphology to be rapidly regained upon rehydration. Isolated cell walls from hydrated and desiccated M. flabellifolius leaves were fractionated into their constituent polymers and the resulting fractions were analyzed for monosaccharide content. Significant differences between hydrated and desiccated states were observed in the water-soluble buffer extract, pectin fractions, and the arabinogalactan protein-rich extract. A marked increase in galacturonic acid was found in the alkali-insoluble pectic fraction. Xyloglucan structure was analyzed and shown to be of the standard dicotyledonous pattern. Immunocytochemical analysis determined the cellular location of the various epitopes associated with cell wall components, including pectin, xyloglucan, and arabinogalactan proteins, in hydrated and desiccated leaf tissue. The most striking observation was a constitutively present high concentration of arabinose, which was associated with pectin, presumably in the form of arabinan polymers. We propose that the arabinan-rich leaf cell wall of M. flabellifolius possesses the necessary structural properties to be able to undergo repeated periods of desiccation and rehydration. PMID:16603665

  2. Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds.

    PubMed

    Roach, Thomas; Beckett, Richard P; Minibayeva, Farida V; Colville, Louise; Whitaker, Claire; Chen, Hongying; Bailly, Christophe; Kranner, Ilse

    2010-01-01

    Reactive oxygen species (ROS) are implicated in seed death following dehydration in desiccation-intolerant 'recalcitrant' seeds. However, it is unknown if and how ROS are produced in the apoplast and if they play a role in stress signalling during desiccation. We studied intracellular damage and extracellular superoxide (O(2)(.-)) production upon desiccation in Castanea sativa seeds, mechanisms of O(2)(.-) production and the effect of exogenously supplied ROS. A transient increase in extracellular O(2)(.-) production by the embryonic axes preceded significant desiccation-induced viability loss. Thereafter, progressively more oxidizing intracellular conditions, as indicated by a significant shift in glutathione half-cell reduction potential, accompanied cell and axis death, coinciding with the disruption of nuclear membranes. Most hydrogen peroxide (H(2)O(2))-dependent O(2)(.-) production was found in a cell wall fraction that contained extracellular peroxidases (ECPOX) with molecular masses of approximately 50 kDa. Cinnamic acid was identified as a potential reductant required for ECPOX-mediated O(2)(.-) production. H(2)O(2), applied exogenously to mimic the transient ROS burst at the onset of desiccation, counteracted viability loss of sub-lethally desiccation-stressed seeds and of excised embryonic axes grown in tissue culture. Hence, extracellular ROS produced by embryonic axes appear to be important signalling components involved in wound response, regeneration and growth.

  3. Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids.

    PubMed

    Dresch, Daiane M; Masetto, Tathiana E; Scalon, Silvana P Q

    2015-01-01

    The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1% or less by desiccation using silica gel, and to 17.2% or less by desiccation under laboratory conditions; impairment of the physiological potential of the seeds was observed at these low moisture content levels. The integrity of the seed genomic DNA was not affected after drying seeds in the two methods. However, drying in silica gel to 4.5% moisture content and drying under laboratory conditions to 5.4% moisture content resulted in the loss of seed RNA integrity. PMID:26648543

  4. Photosynthetic recovery following desiccation of desert green algae (Chlorophyta) and their aquatic relatives.

    PubMed

    Gray, Dennis W; Lewis, Louise A; Cardon, Zoe G

    2007-10-01

    Recent molecular data suggest that desert green algae have evolved from freshwater ancestors at least 14 times in three major classes (Chlorophyceae, Trebouxiophyceae and Charophyceae), offering a unique opportunity to study the adaptation of photosynthetic organisms to life on land in a comparative phylogenetic framework. We examined the photorecovery of phylogenetically matched desert and aquatic algae after desiccation in darkness and under illumination. Desert algae survived desiccation for at least 4 weeks when dried in darkness, and recovered high levels of photosynthetic quantum yield within 1 h of rehydration in darkness. However, when 4 weeks of desiccation was accompanied by illumination, three of six desert taxa lost their ability to recover quantum yield during rehydration in the dark. Aquatic algae, in contrast, recovered very little during dark rehydration following even just 24 h of desiccation. Re-illuminating rehydrated algae produced a nearly complete recovery of quantum yield in all desert and two of five aquatic taxa. These contrasts provide physiological evidence that desert green algae possess mechanisms for photosynthetic recovery after desiccation distinct from those in aquatic relatives, corroborating molecular evidence that they are not happenstance, short-term visitors from aquatic environments. Photosensitivity during desiccation among desert algae further suggests that they may reside in protected microsites within crusts, and species specificity of photosensitivity suggests that disturbances physically disrupting crusts could lead to shifts or losses of taxonomic diversity within these habitats.

  5. Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids.

    PubMed

    Dresch, Daiane M; Masetto, Tathiana E; Scalon, Silvana P Q

    2015-01-01

    The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1% or less by desiccation using silica gel, and to 17.2% or less by desiccation under laboratory conditions; impairment of the physiological potential of the seeds was observed at these low moisture content levels. The integrity of the seed genomic DNA was not affected after drying seeds in the two methods. However, drying in silica gel to 4.5% moisture content and drying under laboratory conditions to 5.4% moisture content resulted in the loss of seed RNA integrity.

  6. A Comparative Study between Solenopsis invicta and Solenopsis richteri on Tolerance to Heat and Desiccation Stresses

    PubMed Central

    Chen, Jian; Rashid, Tahir; Feng, Guolei

    2014-01-01

    Solenopsis invicta and Solenopsis richteri are two very closely related invasive ant species; however, S. invicta is a much more successful invader. Physiological tolerance to abiotic stress has been hypothesized to be important to the success of an invasive species. In this study, we tested the hypothesis that S. invicta is more tolerant to heat and desiccation stress than S. richteri. The data strongly support our hypothesis. S. invicta was found to be significantly less vulnerable than S. richteri to both heat and desiccation stress. Despite S. richteri having significantly higher body water content, S. invicta was less sensitive to desiccation stress due to its significantly lower water loss rate (higher desiccation resistance). After the cuticular lipid was removed, S. invicta still had a significantly lower water loss rate than S. richteri, indicating that cuticular lipids were not the only factors accounting for difference in the desiccation resistance between these two species. Since multiple biological and/or ecological traits can contribute to the invasion success of a particular species, whether the observed difference in tolerance to heat and desiccation stresses is indeed associated with the variation in invasion success between these two species can only be confirmed by further extensive comparative study. PMID:24915009

  7. Calorimetric Properties of Dehydrating Pollen (Analysis of a Desiccation-Tolerant and an Intolerant Species).

    PubMed

    Buitink, J.; Walters-Vertucci, C.; Hoekstra, F. A.; Leprince, O.

    1996-05-01

    The physical state of water in the desiccation-tolerant pollen of Typha latifolia L. and the desiccation-sensitive pollen of Zea mays L. was studied using differential scanning calorimetry in an attempt to further unravel the complex mechanisms of desiccation tolerance. Melting transitions of water were not observed at water content (wc) values less than 0.21 (T. latifolia) and 0.26 (Z. mays) g H2O/g dry weight. At moisture levels at which melting transitions were not observable, water properties could be characterized by changes in heat capacity. Three hydration regions could be distinguished with the defining wc values changing as a function of temperature. Shifts in baseline power resembling second-order transitions were observed in both species and were interpreted as glass-to-liquid transitions, the glass-transition temperatures being dependent on wc. Irrespective of the extent of desiccation tolerance, both pollens exhibited similar state diagrams. The viability of maize pollen at room temperature decreased gradually with the removal of the unfrozen water fraction. In maize, viability was completely lost before grains were sufficiently dried to enter into a glassy state. Apparently, the glassy state per se cannot provide desiccation tolerance. From the existing data, we conclude that, although no major differences in the physical behavior of water could be distinguished between desiccation-tolerant and -intolerant pollens, the physiological response to the loss of water varies between the two pollen types. PMID:12226289

  8. Solar assisted alkali pretreatment of garden biomass: Effects on lignocellulose degradation, enzymatic hydrolysis, crystallinity and ultra-structural changes in lignocellulose

    SciTech Connect

    Gabhane, Jagdish; William, S.P.M. Prince; Vaidya, Atul N.; Das, Sera; Wate, Satish R.

    2015-06-15

    Highlights: • SAAP is an efficient and economic means of pretreatment. • SAAP was found to be efficient in lignin and hemicellulose removal. • SAAP enhanced the enzymatic hydrolysis. • FTIR, XRD and SEM provided vivid understanding about the mode of action of SAAP. • Mass balance closer of 98% for pretreated GB confirmed the reliability of SAAP. - Abstract: A comprehensive study was carried out to assess the effectiveness of solar assisted alkali pretreatment (SAAP) on garden biomass (GB). The pretreatment efficiency was assessed based on lignocellulose degradation, conversion of cellulose into reducing sugars, changes in the ultra-structure and functional groups of lignocellulose and impact on the crystallinity of cellulose, etc. SAAP was found to be efficient for the removal of lignin and hemicellulose that facilitated enzymatic hydrolysis of cellulose. FTIR and XRD studies provided details on the effectiveness of SAAP on lignocellulosic moiety and crystallinity of cellulose. Scanning electron microscopic analysis showed ultra-structural disturbances in the microfibrils of GB as a result of pretreatment. The mass balance closer of 97.87% after pretreatment confirmed the reliability of SAAP pretreatment. Based on the results, it is concluded that SAAP is not only an efficient means of pretreatment but also economical as it involved no energy expenditure for heat generation during pretreatment.

  9. REACTOR COOLING

    DOEpatents

    Quackenbush, C.F.

    1959-09-29

    A nuclear reactor with provisions for selectively cooling the fuel elements is described. The reactor has a plurality of tubes extending throughout. Cylindrical fuel elements are disposed within the tubes and the coolant flows through the tubes and around the fuel elements. The fuel elements within the central portion of the reactor are provided with roughened surfaces of material. The fuel elements in the end portions of the tubes within the reactor are provlded with low conduction jackets and the fuel elements in the region between the central portion and the end portions are provided with smooth surfaces of high heat conduction material.

  10. Gas-fired desiccant system for retail super center

    SciTech Connect

    Spears, J.W.; Judge, J.

    1997-10-01

    Concerns about indoor air quality have led to increasing outside air requirements that have prompted HVAC system designers to rethink how to handle outside air. The resulting increase in latent load can cause a variety of problems such as uncomfortably high humidity, mold and mildew, sweating ducts and higher energy cost. These problems occur not only in very humid climates but also in moderate climates during the swing season when the sensible load is low and the outside humidity is high. This combined with increasing concern for occupant comfort has led engineers to look for HVAC designs that provide good temperature and humidity control while still providing adequate quantities of outside air ventilation. This article describes the results of a one-year monitored evaluation of a gas-fired desiccant makeup air system used in a Wal-Mart super center. The system provides continuous fresh-air ventilation and independent temperature and humidity control. It also demonstrates the potential for energy savings and reduced first cost of the HVAC system. This approach, investigated by the owners` design team and independently monitored and verified in this Gas Research Institute-funded field study, has proven to be a cost-effective solution to meeting the new ventilation standard.

  11. Proteomics of seed development, desiccation tolerance, germination and vigor.

    PubMed

    Wang, Wei-Qing; Liu, Shu-Jun; Song, Song-Quan; Møller, Ian Max

    2015-01-01

    Proteomics, the large-scale study of the total complement of proteins in a given sample, has been applied to all aspects of seed biology mainly using model species such as Arabidopsis or important agricultural crops such as corn and rice. Proteins extracted from the sample have typically been separated and quantified by 2-dimensional polyacrylamide gel electrophoresis followed by liquid chromatography and mass spectrometry to identify the proteins in the gel spots. In this way, qualitative and quantitative changes in the proteome during seed development, desiccation tolerance, germination, dormancy release, vigor alteration and responses to environmental factors have all been studied. Many proteins or biological processes potentially important for each seed process have been highlighted by these studies, which greatly expands our knowledge of seed biology. Proteins that have been identified to be particularly important for at least two of the seed processes are involved in detoxification of reactive oxygen species, the cytoskeleton, glycolysis, protein biosynthesis, post-translational modifications, methionine metabolism, and late embryogenesis-abundant (LEA) proteins. It will be useful for molecular biologists and molecular plant breeders to identify and study genes encoding particularly interesting target proteins with the aim to improve the yield, stress tolerance or other critical properties of our crop species. PMID:25461695

  12. Adiabatic air dehumidification in laminar flow desiccant matrices

    SciTech Connect

    Pesaran, A A

    1987-07-01

    Adiabatic step transient heat- and mass-transfer and pressure drop experimental data were obtained for a dehumidifier test matrix that contained microbead-silica-gel desiccant in a parallel-plate geometry. The data were analyzed and compared with the results of two other test dehumidifiers: a parallel-plate matrix using crushed silica gel, and a staggered, parallel-strip matrix using microbead silica gel. The analysis showed that the overall heat- and mass-transfer Nusselt numbers of the staggered, parallel-strip matrix were about 70% to 80% larger than those of the parallel-plate matrices. It also showed that the solid-side resistance to moisture diffusion in the smaller microbead silica gel was about 45% less than that of crushed silica gel because the particle size was 60% smaller. The ratio of heat- or mass-transfer coefficient to pressure drop of the microbead-silica-gel staggered, parallel-strip matrix was higher than the other two test dehumidifiers. Based on these findings, a dehumidifier using microbead silica-gel in a staggered, parallel-strip geometry can be made more compact than the other combinations. 15 refs., 9 figs., 5 tabs.

  13. Genes Associated with Desiccation and Osmotic Stress in Listeria monocytogenes as Revealed by Insertional Mutagenesis

    PubMed Central

    Hingston, Patricia A.; Piercey, Marta J.

    2015-01-01

    Listeria monocytogenes is a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. To probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700 L. monocytogenes mutants was screened, using a microplate assay, for strains displaying increased or decreased desiccation survival (43% relative humidity, 15°C) in tryptic soy broth (TSB). The desiccation phenotypes of selected mutants were subsequently assessed on food-grade stainless steel (SS) coupons in TSB plus 1% glucose (TSB-glu). Single transposon insertions in mutants exhibiting a change in desiccation survival of >0.5 log CFU/cm2 relative to that of the wild type were determined by sequencing arbitrary PCR products. Strain morphology, motility, and osmotic stress survival (in TSB-glu plus 20% NaCl) were also analyzed. The initial screen selected 129 desiccation-sensitive (DS) and 61 desiccation-tolerant (DT) mutants, out of which secondary screening on SS confirmed 15 DT and 15 DS mutants. Among the DT mutants, seven immotile and flagellum-less strains contained transposons in genes involved in flagellum biosynthesis (fliP, flhB, flgD, flgL) and motor control (motB, fliM, fliY), while others harbored transposons in genes involved in membrane lipid biosynthesis, energy production, potassium uptake, and virulence. The genes that were interrupted in the 15 DS mutants included those involved in energy production, membrane transport, protein metabolism, lipid biosynthesis, oxidative damage control, and putative virulence. Five DT and 14 DS mutants also demonstrated similar significantly (P < 0.05) different survival relative to that of the wild type when exposed to osmotic stress, demonstrating that some genes likely have similar roles in allowing the organism to survive the two water stresses. PMID:26025900

  14. [Photosynthetic activity of Gloiopeltis furcata (intertidal red macroalga) in response to desiccation].

    PubMed

    Liu, Hong-Liang; Liu, Hong-Liang; Li, Xue-Meng; Nan, Guo-Ning; Zhang, Quan-Sheng

    2014-05-01

    In this study, the diurnal change of photosynthesis activity in response to various tidal patterns, the relationship between photosynthetic activity and tissue water content, and the interactive effect of desiccation and irradiance on photosynthetic activity in Gloiopeltis furcata were investigated by using portable pulse amplitude modulated (PAM) fluorometer. Results showed that Fv/Fm decreased more rapidly during the noon low tide than during the morning- or evening low tide. F/Fm decreased slowly at the beginning of desiccation during the morning low tide, but decreased rapidly throughout the evening low tide. Fv/Fm recovered to the initial values on the same day no matter when the low tide occurred, suggesting the occurrence of dynamic photoinhibition. These features endowed G. furcata with an ability to adapt to the periodic desiccation on high intertidal rocks. The maximum (Fv/Fm) and effective (Phi(PSII)) quantum yield declined with the decrease of tissue water content (TWC). However, photosynthetic activity could recover completely when TWC exceeded 6%, showing a strong ability of G. furcata to tolerate desiccation. The relationships between TWC and Fv/Fm and Phi (PS II) as were as follows: F/Fm = 0.68 + (0.44-0.68)/[1 +(TWC/ 66.96)]5 , R2 = 0.99; Phi(PSII) = 0.585 + (0.004-0.585)/[1+(TWC/73)10], R2 = 0.99. ANOVA result further showed that the interactive effect of irradiance and desiccation on photosynthetic activity was significant, and that the photoinhibition degree increased with elevation of irradiation and duration of desiccation. The extreme condition (6 h desiccation at 1000 micromol photons x m(-2) x s(-1)) resulted in a serious photoinhibition, with the longest period of complete recovery for photosynthesis activity.

  15. The use of desiccation to treat Staphylococcus aureus biofilm-infected wounds.

    PubMed

    Park, Eugene; Long, Sarah A; Seth, Akhil K; Geringer, Matthew; Xu, Wei; Chavez-Munoz, Claudia; Leung, Kai; Hong, Seok Jong; Galiano, Robert D; Mustoe, Thomas A

    2016-03-01

    Chronic wounds colonized with biofilm present a major burden to our healthcare system. While the current paradigm for wound healing is to maintain a moist environment, we sought to evaluate the effects of desiccation, and the ability of honey to desiccate wounds, on wound healing characteristics in Staphylococcus aureus biofilm wounds. In vivo biofilm wound healing after exposure to open-air desiccation, honey, molasses, and saline was analyzed using a rabbit ear model of S. aureus biofilm wounds previously developed by our group. Wound morphology was examined using scanning electron microscopy and granulation tissue deposition was measured using light microscopy with hematoxylin and eosin staining. Viable bacterial counts in rabbit ear biofilm wounds and scabs were measured using a drop dilution method. In vitro S. aureus growth curves were established using tryptic soy broth containing honey and glycerol. Gene expression analysis of rabbit ear wounds was performed using reverse transcription quantitative PCR. Rabbit ear S. aureus biofilm wounds exposed to open-air desiccation, honey, and molasses developed a dry scab, which displaced the majority of biofilm bacteria off of the wound bed. Wounds treated with open-air desiccation, honey, and molasses expressed lower levels of the inflammatory markers tumor necrosis factor-α and interleukin-1β at postoperative day 12 compared with wounds treated with saline, and had increased levels of granulation tissue formation. In vitro growth of S. aureus in tryptic soy broth was inhibited by the presence of honey to a greater extent than by the presence of osmolality-matched glycerol. Desiccation of chronic wounds colonized with biofilm via exposure to open air or honey leads to improved wound healing by decreasing bacterial burden and inflammation, and increasing granulation tissue formation. The ability of honey to help heal chronic wounds is at least in part due to its ability to desiccate bacterial biofilm, but other

  16. Desiccation stress and tolerance in green algae: consequences for ultrastructure, physiological and molecular mechanisms

    PubMed Central

    Holzinger, Andreas; Karsten, Ulf

    2013-01-01

    Although most green algae typically occur in aquatic ecosystems, many species also live partly or permanently under aeroterrestrial conditions, where the cells are exposed to the atmosphere and hence regularly experience dehydration. The ability of algal cells to survive in an air-dried state is termed desiccation tolerance. The mechanisms involved in desiccation tolerance of green algae are still poorly understood, and hence the aim of this review is to summarize recent findings on the effects of desiccation and osmotic water loss. Starting from structural changes, physiological, and biochemical consequences of desiccation will be addressed in different green-algal lineages. The available data clearly indicate a range of strategies, which are rather different in streptophycean and non-streptophycean green algae. While members of the Trebouxiophyceae exhibit effective water loss-prevention mechanisms based on the biosynthesis and accumulation of particular organic osmolytes such as polyols, these compounds are so far not reported in representatives of the Streptophyta. In members of the Streptophyta such as Klebsormidium, the most striking observation is the appearance of cross-walls in desiccated samples, which are strongly undulating, suggesting a high degree of mechanical flexibility. This aids in maintaining structural integrity in the dried state and allows the cell to maintain turgor pressure for a prolonged period of time during the dehydration process. Physiological strategies in aeroterrestrial green algae generally include a rapid reduction of photosynthesis during desiccation, but also a rather quick recovery after rewetting, whereas aquatic species are sensitive to drying. The underlying mechanisms such as the affected molecular components of the photosynthetic machinery are poorly understood in green algae. Therefore, modern approaches based on transcriptomics, proteomics, and/or metabolomics are urgently needed to better understand the molecular

  17. Desiccation stress and tolerance in green algae: consequences for ultrastructure, physiological and molecular mechanisms.

    PubMed

    Holzinger, Andreas; Karsten, Ulf

    2013-01-01

    Although most green algae typically occur in aquatic ecosystems, many species also live partly or permanently under aeroterrestrial conditions, where the cells are exposed to the atmosphere and hence regularly experience dehydration. The ability of algal cells to survive in an air-dried state is termed desiccation tolerance. The mechanisms involved in desiccation tolerance of green algae are still poorly understood, and hence the aim of this review is to summarize recent findings on the effects of desiccation and osmotic water loss. Starting from structural changes, physiological, and biochemical consequences of desiccation will be addressed in different green-algal lineages. The available data clearly indicate a range of strategies, which are rather different in streptophycean and non-streptophycean green algae. While members of the Trebouxiophyceae exhibit effective water loss-prevention mechanisms based on the biosynthesis and accumulation of particular organic osmolytes such as polyols, these compounds are so far not reported in representatives of the Streptophyta. In members of the Streptophyta such as Klebsormidium, the most striking observation is the appearance of cross-walls in desiccated samples, which are strongly undulating, suggesting a high degree of mechanical flexibility. This aids in maintaining structural integrity in the dried state and allows the cell to maintain turgor pressure for a prolonged period of time during the dehydration process. Physiological strategies in aeroterrestrial green algae generally include a rapid reduction of photosynthesis during desiccation, but also a rather quick recovery after rewetting, whereas aquatic species are sensitive to drying. The underlying mechanisms such as the affected molecular components of the photosynthetic machinery are poorly understood in green algae. Therefore, modern approaches based on transcriptomics, proteomics, and/or metabolomics are urgently needed to better understand the molecular

  18. Genes Associated with Desiccation and Osmotic Stress in Listeria monocytogenes as Revealed by Insertional Mutagenesis.

    PubMed

    Hingston, Patricia A; Piercey, Marta J; Truelstrup Hansen, Lisbeth

    2015-08-15

    Listeria monocytogenes is a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. To probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700 L. monocytogenes mutants was screened, using a microplate assay, for strains displaying increased or decreased desiccation survival (43% relative humidity, 15°C) in tryptic soy broth (TSB). The desiccation phenotypes of selected mutants were subsequently assessed on food-grade stainless steel (SS) coupons in TSB plus 1% glucose (TSB-glu). Single transposon insertions in mutants exhibiting a change in desiccation survival of >0.5 log CFU/cm(2) relative to that of the wild type were determined by sequencing arbitrary PCR products. Strain morphology, motility, and osmotic stress survival (in TSB-glu plus 20% NaCl) were also analyzed. The initial screen selected 129 desiccation-sensitive (DS) and 61 desiccation-tolerant (DT) mutants, out of which secondary screening on SS confirmed 15 DT and 15 DS mutants. Among the DT mutants, seven immotile and flagellum-less strains contained transposons in genes involved in flagellum biosynthesis (fliP, flhB, flgD, flgL) and motor control (motB, fliM, fliY), while others harbored transposons in genes involved in membrane lipid biosynthesis, energy production, potassium uptake, and virulence. The genes that were interrupted in the 15 DS mutants included those involved in energy production, membrane transport, protein metabolism, lipid biosynthesis, oxidative damage control, and putative virulence. Five DT and 14 DS mutants also demonstrated similar significantly (P < 0.05) different survival relative to that of the wild type when exposed to osmotic stress, demonstrating that some genes likely have similar roles in allowing the organism to survive the two water stresses.

  19. Cellular Biochemical Changes in Selaginella tamariscina (Beauv.) Spring and Sellaginella plana (Desv. ex Poir.) Heiron. as Induced by Desiccation.

    PubMed

    Agduma, Angelo Rellama; Sese, Maribel Dionisio

    2016-08-01

    The biochemical changes in two Selaginella species namely, S. tamariscina (Beauv.) Spring and S. plana (Desv. ex Poir.) Heiron., as induced by desiccation and subsequent rehydration were explored. Plants were allowed to dehydrate naturally by withholding irrigation until shoot's relative water content (RWC) reached <10%. After which, dehydrated plants were watered until fully rehydrated states were obtained which was about 90% RWC or more. Desiccation-tolerance characteristics were observed in S. tamariscina while desiccation-sensitivity features were seen in S. plana. Membrane integrity was maintained in S. tamariscina but not in S. plana as evidenced in the relative electrolyte leakage measurements during desiccation phase and the subsequent rehydration stage. Pigment analyses revealed conservation of some chlorophylls and carotenoids during desiccation and reaching control levels following rehydration in S. tamariscina. Very low pigment contents were found in S. plana during desiccation phase and the pigments were not recovered during rehydration attempt. Meanwhile, compatible solute determination showed rise in total sugar and proline contents of desiccated S. tamariscina only, indicating presence of biochemical protection machineries in this species and absence of such in S. plana during dehydrating conditions. These data indicate that one key element for desiccation-tolerance in lower vascular plants is the ability to protect tissues from severe damages caused by intense desiccation. PMID:27688850

  20. Cellular Biochemical Changes in Selaginella tamariscina (Beauv.) Spring and Sellaginella plana (Desv. ex Poir.) Heiron. as Induced by Desiccation

    PubMed Central

    Agduma, Angelo Rellama; Sese, Maribel Dionisio

    2016-01-01

    The biochemical changes in two Selaginella species namely, S. tamariscina (Beauv.) Spring and S. plana (Desv. ex Poir.) Heiron., as induced by desiccation and subsequent rehydration were explored. Plants were allowed to dehydrate naturally by withholding irrigation until shoot’s relative water content (RWC) reached <10%. After which, dehydrated plants were watered until fully rehydrated states were obtained which was about 90% RWC or more. Desiccation-tolerance characteristics were observed in S. tamariscina while desiccation-sensitivity features were seen in S. plana. Membrane integrity was maintained in S. tamariscina but not in S. plana as evidenced in the relative electrolyte leakage measurements during desiccation phase and the subsequent rehydration stage. Pigment analyses revealed conservation of some chlorophylls and carotenoids during desiccation and reaching control levels following rehydration in S. tamariscina. Very low pigment contents were found in S. plana during desiccation phase and the pigments were not recovered during rehydration attempt. Meanwhile, compatible solute determination showed rise in total sugar and proline contents of desiccated S. tamariscina only, indicating presence of biochemical protection machineries in this species and absence of such in S. plana during dehydrating conditions. These data indicate that one key element for desiccation-tolerance in lower vascular plants is the ability to protect tissues from severe damages caused by intense desiccation.

  1. Cellular Biochemical Changes in Selaginella tamariscina (Beauv.) Spring and Sellaginella plana (Desv. ex Poir.) Heiron. as Induced by Desiccation

    PubMed Central

    Agduma, Angelo Rellama; Sese, Maribel Dionisio

    2016-01-01

    The biochemical changes in two Selaginella species namely, S. tamariscina (Beauv.) Spring and S. plana (Desv. ex Poir.) Heiron., as induced by desiccation and subsequent rehydration were explored. Plants were allowed to dehydrate naturally by withholding irrigation until shoot’s relative water content (RWC) reached <10%. After which, dehydrated plants were watered until fully rehydrated states were obtained which was about 90% RWC or more. Desiccation-tolerance characteristics were observed in S. tamariscina while desiccation-sensitivity features were seen in S. plana. Membrane integrity was maintained in S. tamariscina but not in S. plana as evidenced in the relative electrolyte leakage measurements during desiccation phase and the subsequent rehydration stage. Pigment analyses revealed conservation of some chlorophylls and carotenoids during desiccation and reaching control levels following rehydration in S. tamariscina. Very low pigment contents were found in S. plana during desiccation phase and the pigments were not recovered during rehydration attempt. Meanwhile, compatible solute determination showed rise in total sugar and proline contents of desiccated S. tamariscina only, indicating presence of biochemical protection machineries in this species and absence of such in S. plana during dehydrating conditions. These data indicate that one key element for desiccation-tolerance in lower vascular plants is the ability to protect tissues from severe damages caused by intense desiccation. PMID:27688850

  2. Thermal and economic assessment of hot side sensible heat and cold side phase change storage combination fo absorption solar cooling system

    NASA Astrophysics Data System (ADS)

    Choi, M. K.; Morehouse, J. H.

    An analysis of a solar assisted absorption cooling system which employs a combination of phase change on the cold side and sensible heat storage on the hot side of the cooling machine for small commercial buildings is given. The year-round thermal performance of this system for space cooling were determined by simulation and compared against conventional cooling systems in three geographic locations: Phoenix, Arizona; Miami, Florida and Washington, D.C. The results indicate that the hot-cold storage combination has a considerable amount of energy and economical savings over hot side sensible heat storage. Using the hot-cold storage combination, the optimum collector areas for Washington, D.C., Phoenix and Miami are 355 m squared, 250 m squared and 495 m squared, respectively. Compared against conventional vapor compression chiller, the net solar fractions are 61, 67 and 69 percent, respectively.

  3. Assessment of desiccants and their instructions for use in rapid diagnostic tests

    PubMed Central

    2012-01-01

    Background Malaria rapid diagnostic tests (RDTs) are protected from humidity-caused degradation by a desiccant added to the device packaging. The present study assessed malaria RDT products for the availability, type and design of desiccants and their information supplied in the instructions for use (IFU). Methods Criteria were based on recommendations of the World Health Organization (WHO), the European Community (CE) and own observations. Silica gel sachets were defined as self-indicating (all beads coated with a humidity indicator that changes colour upon saturation), partial-indicating (part of beads coated) and non-indicating (none of the beads coated). Indicating silica gel sachets were individually assessed for humidity saturation and (in case of partial-indicating silica gels) for the presence of indicating beads. Results Fifty malaria RDT products from 25 manufacturers were assessed, 14 (28%) products were listed by the “Global Fund Quality Assurance Policy” and 31 (62%) were CE-marked. All but one product contained a desiccant, mostly (47/50, 94%) silica gel. Twenty (40%) RDT products (one with no desiccant and 19 with non-indicating desiccant) did not meet the WHO guidelines recommending indicating desiccant. All RDT products with self- or partial-indicating silica gel (n = 22 and 8 respectively) contained the toxic cobalt dichloride as humidity indicator. Colour change indicating humidity saturation was observed for 8/16 RDT products, at a median incidence of 0.8% (range 0.05%-4.6%) of sachets inspected. In all RDTs with partial-indicating silica gel, sachets with no colour indicating beads were found (median proportion 13.5% (0.6% - 17.8%) per product) and additional light was needed to assess the humidity colour. Less than half (14/30, 47%) IFUs of RDT products with indicating desiccants mentioned to check the humidity saturation before using the test. Information on properties, safety hazards and disposal of the desiccant was not included in

  4. The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae

    NASA Astrophysics Data System (ADS)

    Leedham Elvidge, E. C.; Phang, S.-M.; Sturges, W. T.; Malin, G.

    2015-01-01

    Exposure of intertidal macroalgae during low tide has been linked to the emission of a variety of atmospherically-important trace gases into the coastal atmosphere. In recent years, several studies have investigated the role of inorganic iodine and organoiodides as antioxidants and their emission during exposure to combat oxidative stress, yet the role of organic bromine species during desiccation is less well understood. In this study the emission of dibromomethane (CH2Br2) and bromoform (CHBr3) during exposure and desiccation of two common temperate macroalgae, Fucus vesiculosus and Ulva intestinalis, is reported. Determination of the impact exposure may have on algal physiological processes is difficult as intertidal species are adapted to desiccation and may undergo varying degrees of desiccation before their physiology is affected. For this reason we include comparisons between photosynthetic capacity (Fv/Fm) and halocarbon emissions during a desiccation time series. In addition, the role of rewetting with freshwater to simulate exposure to rain was also investigated. Our results show that an immediate flux of bromocarbons occurs upon exposure, followed by a decline in bromocarbon emissions. We suggest that this immediate bromocarbon pulse may be linked to volatilisation or emissions of existing bromocarbon stores from the algal surface rather than the production of bromocarbons as an antioxidant response.

  5. The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae

    NASA Astrophysics Data System (ADS)

    Leedham Elvidge, E. C.; Phang, S.-M.; Sturges, W. T.; Malin, G.

    2014-07-01

    Exposure of intertidal macroalgae during low tide has been linked to the emission of a variety of atmospherically-important trace gases into the coastal atmosphere. In recent years, several studies have investigated the role of inorganic iodine and organoiodides as antioxidants and their emission during exposure to combat oxidative stress, yet the role of organic bromine species during desiccation is less well understood. In this study the emission of dibromomethane (CH2Br2) and bromoform (CHBr3) during exposure and desiccation of two common temperate macroalgae, Fucus vesiculosus and Ulva intestinalis, is reported. Determination of the impact exposure may have on algal physiological processes is difficult as intertidal species are adapted to desiccation and may undergo varying degrees of desiccation before their physiology is affected. For this reason we include comparisons between photosynthetic capacity (Fv / Fm) and halocarbon emissions during a desiccation time series. In addition, the role of rewetting with freshwater to simulate exposure to rain was also investigated. Our results show that an immediate flux of bromocarbons occurs upon exposure, followed by a decline in bromocarbon emissions. We suggest that this immediate bromocarbon pulse may be linked to volatilisation or emissions of existing bromocarbon stores from the algal surface rather than the production of bromocarbons as an antioxidant response.

  6. Intraspecific variation in desiccation survival time of Aedes aegypti (L.) mosquito eggs of Australian origin.

    PubMed

    Faull, Katherine J; Williams, Craig R

    2015-12-01

    Aedes aegypti (L.) mosquitoes preferentially oviposit in natural and artificial receptacles where their eggs are able to withstand drying as water levels fluctuate. Desiccation-resistant eggs also increase the potential for establishment in non-native habitats while providing logistical impediments to control programs. Viability and mean survival times of eggs stored under three dryness conditions for up to 367 days were investigated among three field-derived colonies of Australian Ae. aegypti to understand variation in desiccation survival. Further investigations compared egg survival between an established colony and its wild counterpart. Our results confirmed that Ae. aegypti eggs can withstand desiccation for extended periods of time with approximately 2-15% egg viability recorded after one year and viability remaining above 88% under all conditions through 56 days. Intraspecific variations in egg survival times were recorded, suggesting local adaptation while each of the colonies demonstrated a consistent preference for higher humidity. Egg volume varied between the populations, suggesting a relationship between egg volume and survival time, with the marginally larger eggs (Charters Towers and Innisfail) having greater desiccation resistance over the range of conditions. The strong survivorship of Charters Towers eggs in dry, warm conditions demonstrates the adaptive significance of a desiccation-resistant egg. PMID:26611964

  7. Rapid Reactivation of Cyanobacterial Photosynthesis and Migration upon Rehydration of Desiccated Marine Microbial Mats

    PubMed Central

    Chennu, Arjun; Grinham, Alistair; Polerecky, Lubos; de Beer, Dirk; Al-Najjar, Mohammad A. A.

    2015-01-01

    Desiccated cyanobacterial mats are the dominant biological feature in the Earth’s arid zones. While the response of desiccated cyanobacteria to rehydration is well-documented for terrestrial systems, information about the response in marine systems is lacking. We used high temporal resolution hyperspectral imaging, liquid chromatography, pulse-amplitude fluorometry, oxygen microsensors, and confocal laser microscopy to study this response in a desiccated microbial mat from Exmouth Gulf, Australia. During the initial 15 min after rehydration chlorophyll a concentrations increased 2–5 fold and cyanobacterial photosynthesis was re-established. Although the mechanism behind this rapid increase of chlorophyll a remains unknown, we hypothesize that it involves resynthesis from a precursor stored in desiccated cyanobacteria. The subsequent phase (15 min–48 h) involved migration of the reactivated cyanobacteria toward the mat surface, which led, together with a gradual increase in chlorophyll a, to a further increase in photosynthesis. We conclude that the response involving an increase in chlorophyll a and recovery of photosynthetic activity within minutes after rehydration is common for cyanobacteria from desiccated mats of both terrestrial and marine origin. However, the response of upward migration and its triggering factor appear to be mat-specific and likely linked to other factors. PMID:26733996

  8. Effects of pre-harvest chemical application on rice desiccation and seed quality*

    PubMed Central

    HE, Yong-qi; CHENG, Jin-ping; LIU, Liang-feng; LI, Xiao-dan; YANG, Bin; ZHANG, Hong-sheng; WANG, Zhou-fei

    2015-01-01

    Pre-harvest desiccation may increase the efficiency of seed production. Field studies were conducted to determine the effects of diquat, paraquat, and ethephon applications on grain moisture, grain weight, and seed germination of hybrid rice Yanliangyou 88 (Oryza sativa ssp. indica) and conventional rice Wuyunjing 7 (Oryza sativa ssp. japonica). In 2013, we tested 12 treatments applied at four weeks (Yanliangyou 88) and six weeks (Wuyunjing 7) after heading. Results showed that reductions in moisture content were significant two and four days after chemical application. Chemical applications had no adverse effects on 1000-grain weight, germination percentage, or germination index, but there were negative effects on the percentage of normal seedlings. Desiccation effects increased with increase in the period after application, while the effect of ethephon combined with diquat or paraquat on desiccation was limited compared with that of diquat or paraquat alone in a short period after application. In 2013, chemical applications reduced the moisture content by from 0.5% to 6.4%, the germination percentage by from 0% to 3.3%, and the percentage of normal seedlings by from 13.3% to 100.0%. Among the treatments, diquat applied at 120 g/ha resulted in effective desiccation with fewer negative effects on grain weight and seed germination in 2013 and 2014. Therefore, diquat may have potential as a pre-harvest chemical desiccation treatment for rice. These results may provide a basis for developing and implementing protocols for large scale field trials. PMID:26465129

  9. Two Hymenophyllaceae species from contrasting natural environments exhibit a homoiochlorophyllous strategy in response to desiccation stress.

    PubMed

    Flores-Bavestrello, Alejandra; Król, Marianna; Ivanov, Alexander G; Hüner, Norman P A; García-Plazaola, José Ignacio; Corcuera, Luis J; Bravo, León A

    2016-02-01

    Hymenophyllaceae is a desiccation tolerant family of Pteridophytes which are poikilohydric epiphytes. Their fronds are composed by a single layer of cells and lack true mesophyll cells and stomata. Although they are associated with humid and shady environments, their vertical distribution varies along the trunk of the host plant with some species inhabiting the drier sides with a higher irradiance. The aim of this work was to compare the structure and function of the photosynthetic apparatus during desiccation and rehydration in two species, Hymenophyllum dentatum and Hymenoglossum cruentum, isolated from a contrasting vertical distribution along the trunk of their hosts. Both species were subjected to desiccation and rehydration kinetics to analyze frond phenotypic plasticity, as well as the structure, composition and function of the photosynthetic apparatus. Minimal differences in photosynthetic pigments were observed upon dehydration. Measurements of ϕPSII (effective quantum yield of PSII), ϕNPQ (quantum yield of the regulated energy dissipation of PSII), ϕNO (quantum yield of non-regulated energy dissipation of PSII), and TL (thermoluminescence) indicate that both species convert a functional photochemical apparatus into a structure which exhibits maximum quenching capacity in the dehydrated state with minimal changes in photosynthetic pigments and polypeptide compositions. This dehydration-induced conversion in the photosynthetic apparatus is completely reversible upon rehydration. We conclude that H. dentatum and H. cruentum are homoiochlorophyllous with respect to desiccation stress and exhibited no correlation between inherent desiccation tolerance and the vertical distribution along the host tree trunk. PMID:26720213

  10. Rapid Reactivation of Cyanobacterial Photosynthesis and Migration upon Rehydration of Desiccated Marine Microbial Mats.

    PubMed

    Chennu, Arjun; Grinham, Alistair; Polerecky, Lubos; de Beer, Dirk; Al-Najjar, Mohammad A A

    2015-01-01

    Desiccated cyanobacterial mats are the dominant biological feature in the Earth's arid zones. While the response of desiccated cyanobacteria to rehydration is well-documented for terrestrial systems, information about the response in marine systems is lacking. We used high temporal resolution hyperspectral imaging, liquid chromatography, pulse-amplitude fluorometry, oxygen microsensors, and confocal laser microscopy to study this response in a desiccated microbial mat from Exmouth Gulf, Australia. During the initial 15 min after rehydration chlorophyll a concentrations increased 2-5 fold and cyanobacterial photosynthesis was re-established. Although the mechanism behind this rapid increase of chlorophyll a remains unknown, we hypothesize that it involves resynthesis from a precursor stored in desiccated cyanobacteria. The subsequent phase (15 min-48 h) involved migration of the reactivated cyanobacteria toward the mat surface, which led, together with a gradual increase in chlorophyll a, to a further increase in photosynthesis. We conclude that the response involving an increase in chlorophyll a and recovery of photosynthetic activity within minutes after rehydration is common for cyanobacteria from desiccated mats of both terrestrial and marine origin. However, the response of upward migration and its triggering factor appear to be mat-specific and likely linked to other factors. PMID:26733996

  11. Sealed tube comparisons of the compatibility of desiccants with refrigerants and lubricants

    SciTech Connect

    Field, J.E.

    1994-04-01

    Continuing environmental concerns mandate replacement of CFC`s with alternate refrigeration fluids. At this time relatively little testing has been reported in the literature for compatibility of desiccants in these new working fluids. Work has begun, and some results are reported, on a project that will determine the compatibility of virtually all of the currently used desiccant types -- both bead and molded core, with thirteen refrigerant/lubricant combinations. The desiccants are tested by exposure to refrigerant and lubricant in sealed, glass tubes in accordance with ASHRAE/ANSI Standard 97-1989. After aging the lubricants are evaluated for change in color and acid formation. The refrigerants are analyzed for charges by gas chromatography and halide ion formation using ion chromatography. The desiccants are evaluated for changes in crush strength, and for retention of acids and halide ions. Metal catalysts, also present in the sealed tubes, are visually examined for corrosion, copper plating, and appearance change. Results are reported for 4{Angstrom} and 3{Angstrom} molecular sieve desiccants aged in R-12, R-134a and R-32.

  12. Resurrection kinetics of photosynthesis in desiccation-tolerant terrestrial green algae (Chlorophyta) on tree bark.

    PubMed

    Lüttge, U; Büdel, B

    2010-05-01

    The rough bark of orchard trees (Malus) around Darmstadt is predominantly covered in red to purple-brown layers (biofilms) of epiphytic terrestrial alga of Trentepohlia umbrina. The smooth bark of forest trees (Fagus sylvatica L. and Acer sp.) in the same area is covered by bright green biofilms composed of the green algae Desmococcus, Apatococcus and Trebouxia, with a few cells of Coccomyxa and 'Chlorella' trebouxioides between them. These algae are desiccation tolerant. After samples of bark with the biofilms were kept in dry air in darkness for various periods of time, potential quantum yield of PSII, F(v)/F(m), recovered during rehydration upon rewetting. The kinetics and degree of recovery depended on the length of time that the algae were kept in dry air in the desiccated state. Recovery was better for green biofilm samples, i.e. quite good even after 80 days of desiccation (F(v)/F(m) = ca. 50% of initial value), than the red samples, where recovery was only adequate up to ca. 30-40 days of desiccation (F(v)/F(m) = ca. 20-55% of initial value). It is concluded that the different bark types constitute different ecophysiological niches that can be occupied by the algae and that can be distinguished by their capacity to recover from desiccation after different times in the dry state.

  13. Intraspecific variation in desiccation survival time of Aedes aegypti (L.) mosquito eggs of Australian origin.

    PubMed

    Faull, Katherine J; Williams, Craig R

    2015-12-01

    Aedes aegypti (L.) mosquitoes preferentially oviposit in natural and artificial receptacles where their eggs are able to withstand drying as water levels fluctuate. Desiccation-resistant eggs also increase the potential for establishment in non-native habitats while providing logistical impediments to control programs. Viability and mean survival times of eggs stored under three dryness conditions for up to 367 days were investigated among three field-derived colonies of Australian Ae. aegypti to understand variation in desiccation survival. Further investigations compared egg survival between an established colony and its wild counterpart. Our results confirmed that Ae. aegypti eggs can withstand desiccation for extended periods of time with approximately 2-15% egg viability recorded after one year and viability remaining above 88% under all conditions through 56 days. Intraspecific variations in egg survival times were recorded, suggesting local adaptation while each of the colonies demonstrated a consistent preference for higher humidity. Egg volume varied between the populations, suggesting a relationship between egg volume and survival time, with the marginally larger eggs (Charters Towers and Innisfail) having greater desiccation resistance over the range of conditions. The strong survivorship of Charters Towers eggs in dry, warm conditions demonstrates the adaptive significance of a desiccation-resistant egg.

  14. Drying R-407C and R-410A refrigerant blends with molecular sieve desiccants

    SciTech Connect

    Cohen, A.P.; Tucker, D.M.

    1998-10-01

    The hydrofluorocarbon (HFC) R-32 (CF{sub 2}H{sub 2}) is a component of refrigerant blends in the 407 and 410 series being tested and commercialized for use as replacements for R-502 and the hydrochlorofluorocarbon (HCFC) R-22. The molecular sieve desiccants used with chlorofluorocarbon (CFC) and HCFC mineral oil systems in the past have achieved high water capacity by excluding the refrigerant and adsorbing only the water. Unfortunately, R-32 is adsorbed on commercial type 3A molecular sieve desiccant products. The result of this adsorption is a loss of water capacity when drying R-32 compared to drying R-22 or R-502 and a reduced level of chemical compatibility of the desiccant with the refrigerant. Some compressor manufacturers are seeking a water concentration as low as 10 mg/kg (ppm[wt]) in the circulating refrigerant of polyolester-lubricated refrigerating equipment using these HFC blends. This paper compares unmodified commercial type 3A molecular sieve desiccants with a recently developed, modified 3A molecular sieve that excludes R-32. The modified 3A has better chemical compatibility with R-32 and high water capacity in liquid R-407C and R-410A. The drying rates of the two desiccants in R-407C and R-410A are similar. Data and test methods are reported on refrigerant adsorption, water capacity, drying rate, and chemical compatibility.

  15. Surviving metabolic arrest: photosynthesis during desiccation and rehydration in resurrection plants.

    PubMed

    Challabathula, Dinakar; Puthur, Jos T; Bartels, Dorothea

    2016-02-01

    Photosynthesis is the key process that is affected by dehydration in plants. Desiccation-tolerant resurrection plants can survive conditions of very low relative water content. During desiccation, photosynthesis is not operational, but is recovered within a short period after rehydration. While homoiochlorophyllous resurrection plants retain their photosynthetic apparatus during desiccation, poikilochlorophyllous resurrection species dismantle chloroplasts and degrade chlorophyll but resynthesize them again during rehydration. Dismantling the chloroplasts avoids the photooxidative stress in poikilochlorophyllous resurrection plants, whereas it is minimized in homoiochlorophyllous plants through the synthesis of antioxidant enzymes and protective proteins or metabolites. Although the cellular protection mechanisms in both of these species vary, these mechanisms protect cells from desiccation-induced damage and restore photosynthesis upon rehydration. Several of the proteins synthesized during dehydration are localized in chloroplasts and are believed to play major roles in the protection of photosynthetic structures and in recovery in resurrection species. This review focuses on the strategies of resurrection plants in terms of how they protect their photosynthetic apparatus from oxidative stress during desiccation without membrane damage and with full recovery during rehydration. We review the role of the dehydration-induced protection mechanisms in chloroplasts and how photosynthesis is restored during rehydration.

  16. Do subtoxic levels of chlorate influence the desiccation tolerance of Egeria densa?

    PubMed

    Palma, Alvaro T; Schwarz, Alex; Henríquez, Luís A; Alvarez, Ximena; Fariña, José M; Lu, Qimiao

    2013-02-01

    Among the different factors hypothesized to be responsible for the virtual disappearance of Egeria densa, once a dominant aquatic macrophyte in a southern Chile wetland ecosystem, are the negative effects of certain chemical compounds (mainly chlorate) and harsh environmental conditions (desiccation caused by prolonged atmospheric exposure). The authors performed an integrated experiment in which E. densa plants were first exposed for four weeks inside a mesocosm system to levels of chlorate that existed in the wetland at the time of the plant's demise and then exposed to desiccation conditions that also resembled those that the system had experienced. Hence, the authors tested the hypothesis that E. densa plants exposed to sublethal levels of chlorate are more susceptible to the deleterious effect of desiccation compared with plants that had not been exposed to chlorate. This hypothesis was tested by means of quantifying physiologically related parameters in plants right after the four weeks under water and then after the desiccation period of 6 h. Their results rejected this hypothesis, because all plants, regardless of their history, are equally affected by desiccation. PMID:23161751

  17. Effects of pre-harvest chemical application on rice desiccation and seed quality.

    PubMed

    He, Yong-qi; Cheng, Jin-ping; Liu, Liang-feng; Li, Xiao-dan; Yang, Bin; Zhang, Hong-sheng; Wang, Zhou-fei

    2015-10-01

    Pre-harvest desiccation may increase the efficiency of seed production. Field studies were conducted to determine the effects of diquat, paraquat, and ethephon applications on grain moisture, grain weight, and seed germination of hybrid rice Yanliangyou 88 (Oryza sativa ssp. indica) and conventional rice Wuyunjing 7 (Oryza sativa ssp. japonica). In 2013, we tested 12 treatments applied at four weeks (Yanliangyou 88) and six weeks (Wuyunjing 7) after heading. Results showed that reductions in moisture content were significant two and four days after chemical application. Chemical applications had no adverse effects on 1000-grain weight, germination percentage, or germination index, but there were negative effects on the percentage of normal seedlings. Desiccation effects increased with increase in the period after application, while the effect of ethephon combined with diquat or paraquat on desiccation was limited compared with that of diquat or paraquat alone in a short period after application. In 2013, chemical applications reduced the moisture content by from 0.5% to 6.4%, the germination percentage by from 0% to 3.3%, and the percentage of normal seedlings by from 13.3% to 100.0%. Among the treatments, diquat applied at 120 g/ha resulted in effective desiccation with fewer negative effects on grain weight and seed germination in 2013 and 2014. Therefore, diquat may have potential as a pre-harvest chemical desiccation treatment for rice. These results may provide a basis for developing and implementing protocols for large scale field trials.

  18. SOIL DESICCATION TECHNIQUES STRATEGIES FOR IMMOBILIZATION OF DEEP VADOSE CONTAMINANTS AT THE HANFORD CENTRAL PLATEAU

    SciTech Connect

    BENECKE MW; CHRONISTER GB; TRUEX MJ

    2012-01-30

    Deep vadose zone contamination poses some of the most difficult remediation challenges for the protection of groundwater at the Hanford Site where processes and technologies are being developed and tested for use in the on-going effort to remediate mobile contamination in the deep vadose zone, the area deep beneath the surface. Historically, contaminants were discharged to the soil along with significant amounts of water, which continues to drive contaminants deeper in the vadose zone toward groundwater. Soil desiccation is a potential in situ remedial technology well suited for the arid conditions and the thick vadose zone at the Hanford Site. Desiccation techniques could reduce the advance of contaminants by removing the pore water to slow the rate of contaminants movement toward groundwater. Desiccation technologies have the potential to halt or slow the advance of contaminants in unsaturated systems, as well as aid in reduction of contaminants from these same areas. Besides reducing the water flux, desiccation also establishes capillary breaks that would require extensive rewetting to resume pore water transport. More importantly, these techniques have widespread application, whether the need is to isolate radio nuclides or address chemical contaminant issues. Three different desiccation techniques are currently being studied at Hanford.

  19. Negative effects of desiccation on the protein sorting and post-translational modification.

    PubMed

    Wang, Xiaoqin; He, Yikun

    2009-05-01

    Bryophytes as the first land plants are believed to have colonized the land from a fresh water origin, requiring adaptive mechanisms that survival of dehydration. Physcomitrella patens is such a non-vascular bryophyte and shows rare desiccation tolerance in its vegetative tissues. Previous studies showed that during the course of dehydration, several related processes are set in motion: plasmolysis, chloroplast remodeling and microtubule depolymerization. And proteomic alteration supported the cellular structural changes in respond to desiccation stress. In this addendum, we report that Golgi bodies are absent and adaptor protein complex AP-1 large subunit is downregulated during the course of dehydration. Those phenomena may be adverse in protein posttranslational modification, protein sorting and cell walls synthesis under the desiccation condition. PMID:19816114

  20. An Overview of the Biology of the Desiccation-tolerant Resurrection Plant Myrothamnus flabellifolia

    PubMed Central

    Moore, John P.; Lindsey, George G.; Farrant, Jill M.; Brandt, Wolf F.

    2007-01-01

    Background Myrothamnus flabellifolia is unique as the only woody resurrection plant. It is an important plant in southern Africa because of its widespread occurrence and usage in African medicine and traditional culture. Many reports have investigated facets of its biology and the mechanisms associated with its desiccation tolerance. Scope The general biology of the woody resurrection plant Myrothamnus flabellifolia is reviewed. The review focuses on the geography and ecology, systematic placement, evolution, morphology and reproductive ecology of M. flabellifolia as well as the wood anatomy and re-filling mechanism. In addition, the desiccation tolerance, ethnobotanical importance and medicinal properties of the plant are reviewed. Also, future research avenues are suggested, in particular the necessity to research the biogeography and systematics of the species and the role of the polyphenols present, as well as the molecular basis of the plant's desiccation tolerance. PMID:17218343

  1. Induction of tolerance to desiccation and cryopreservation in silver maple (Acer saccharinum) embryonic axes.

    PubMed

    Beardmore, T; Whittle, C-A

    2005-08-01

    Twenty percent of of the world's flowering plants produce recalcitrant seeds (i.e., seeds that cannot withstand drying or freezing). We investigated whether the embryonic axis from the normally recalcitrant seeds of silver maple (Acer saccharinum L.) can be made tolerant to desiccation (10% water content) and low temperature (-196 degrees C, cryopreservation) by pretreatment with ABA or the compound tetcyclacis, which enhances endogenous ABA concentrations. Pretreatment of axes with both ABA and tetcyclacis increased germination after desiccation and freezing to 55% from a control value of zero. Pretreatment of axes with ABA and tetcyclacis increased the ABA content of the axes, as measured by enzyme-linked immunoassay, and stimulated the synthesis of storage and dehydrin-like proteins, believed to have a role in the desiccation tolerance of orthodox seeds.

  2. Induction of tolerance to desiccation and cryopreservation in silver maple (Acer saccharinum) embryonic axes.

    PubMed

    Beardmore, T; Whittle, C-A

    2005-08-01

    Twenty percent of of the world's flowering plants produce recalcitrant seeds (i.e., seeds that cannot withstand drying or freezing). We investigated whether the embryonic axis from the normally recalcitrant seeds of silver maple (Acer saccharinum L.) can be made tolerant to desiccation (10% water content) and low temperature (-196 degrees C, cryopreservation) by pretreatment with ABA or the compound tetcyclacis, which enhances endogenous ABA concentrations. Pretreatment of axes with both ABA and tetcyclacis increased germination after desiccation and freezing to 55% from a control value of zero. Pretreatment of axes with ABA and tetcyclacis increased the ABA content of the axes, as measured by enzyme-linked immunoassay, and stimulated the synthesis of storage and dehydrin-like proteins, believed to have a role in the desiccation tolerance of orthodox seeds. PMID:15929927

  3. DESICCATION INDEX: A MEASURE OF DAMAGE CAUSED BY ADVERSE AERIAL EXPOSURE ON INTERTIDAL EELGRASS (ZOSTERA MARINA) IN AN OREGON (USA) ESTUARY

    EPA Science Inventory

    Eelgrass (Zostera marina) blade necrosis resulting from intertidal aerial exposure is describe. A desiccation index was developed to quantitatively assess this damage. This index was then used to evaluate the extent of desiccation damage across intertidal bathymetric slopes (st...

  4. Addition of Alarm Pheromone Components Improves the Effectiveness of Desiccant Dusts Against Cimex lectularius

    PubMed Central

    BENOIT, JOSHUA B.; PHILLIPS, SETH A.; CROXALL, TRAVIS J.; CHRISTENSEN, BRADY S.; YODER, JAY A.; DENLINGER, DAVID L.

    2009-01-01

    We demonstrate that the addition of bed bug, Cimex lectularius, alarm pheromone to desiccant formulations greatly enhances their effectiveness during short-term exposure. Two desiccant formulations, diatomaceous earth (DE) and Dri-die (silica gel), were applied at the label rate with and without bed bug alarm pheromone components, (E)-2-hexenal, (E)-2-octenal, and a (E)-2-hexenal:(E)-2-octenal blend. First-instar nymphs and adult females were subjected to 10-min exposures, and water loss rates were used to evaluate the response. Optimal effectiveness was achieved with a pheromone concentration of 0.01 M. With Dri-die alone, the water loss was 21% higher than in untreated controls, and water loss increased nearly two times with (E)-2-hexenal and (E)-2-octenal and three times with the (E)-2-hexenal: (E)-2-octenal blend. This shortened survival of first-instar nymphs from 4 to 1 d, with a similar reduction noted in adult females. DE was effective only if supplemented with pheromone, resulting in a 50% increase in water loss over controls with the (E)-2-hexenal:(E)-2-octenal blend, and a survival decrease from 4 to 2 d in first-instar nymphs. Consistently, the addition of the pheromone blend to desiccant dust was more effective than adding either component by itself or by using Dri-die or DE alone. Based on observations in a small microhabitat, the addition of alarm pheromone components prompted bed bugs to leave their protective harborages and to move through the desiccant, improving the use of desiccants for control. We concluded that short exposure to Dri-die is a more effective treatment against bed bugs than DE and that the effectiveness of the desiccants can be further enhanced by incorporation of alarm pheromone. Presumably, the addition of alarm pheromone elevates excited crawling activity, thereby promoting cuticular changes that increase water loss. PMID:19496429

  5. Addition of alarm pheromone components improves the effectiveness of desiccant dusts against Cimex lectularius.

    PubMed

    Benoit, Joshua B; Phillips, Seth A; Croxall, Travis J; Christensen, Brady S; Yoder, Jay A; Denlinger, David L

    2009-05-01

    We demonstrate that the addition of bed bug, Cimex lectularius, alarm pheromone to desiccant formulations greatly enhances their effectiveness during short-term exposure. Two desiccant formulations, diatomaceous earth (DE) and Dri-die (silica gel), were applied at the label rate with and without bed bug alarm pheromone components, (E)-2-hexenal, (E)-2-octenal, and a (E)-2-hexenal:(E)-2-octenal blend. First-instar nymphs and adult females were subjected to 10-min exposures, and water loss rates were used to evaluate the response. Optimal effectiveness was achieved with a pheromone concentration of 0.01 M. With Dri-die alone, the water loss was 21% higher than in untreated controls, and water loss increased nearly two times with (E)-2-hexenal and (E)-2-octenal and three times with the (E)-2-hexenal: (E)-2-octenal blend. This shortened survival of first-instar nymphs from 4 to 1 d, with a similar reduction noted in adult females. DE was effective only if supplemented with pheromone, resulting in a 50% increase in water loss over controls with the (E)-2-hexenal:(E)-2-octenal blend, and a survival decrease from 4 to 2 d in first-instar nymphs. Consistently, the addition of the pheromone blend to desiccant dust was more effective than adding either component by itself or by using Dri-die or DE alone. Based on observations in a small microhabitat, the addition of alarm pheromone components prompted bed bugs to leave their protective harborages and to move through the desiccant, improving the use of desiccants for control. We concluded that short exposure to Dri-die is a more effective treatment against bed bugs than DE and that the effectiveness of the desiccants can be further enhanced by incorporation of alarm pheromone. Presumably, the addition of alarm pheromone elevates excited crawling activity, thereby promoting cuticular changes that increase water loss.

  6. Desiccation tolerance of Hymenophyllacea filmy ferns is mediated by constitutive and non-inducible cellular mechanisms.

    PubMed

    Garcés Cea, Marcelo; Claverol, Stephan; Alvear Castillo, Carla; Rabert Pinilla, Claudia; Bravo Ramírez, León

    2014-04-01

    The Hymenophyllaceae is a primitive family within the Filicopsidae. One of the most exceptional features of this family of ferns is the presence of fronds with one or just a few cell layers (hence their name of filmy ferns), and the absence of stomata. Hymenophyllum caudiculatum and Hymenophyllum dentatum are able to lose more than 82% of their fully hydrated water content, to remain dry for extended periods of time (days or weeks), and to survive and remain viable following rehydration. The aim of this work was to understand whether the adaptive strategy of the Hymenophyllaceae for desiccation tolerance is constitutive or inducible. A proteomic approach was adopted in combination with physiological parameters to assess whether there were changes in the protein content during dehydration and following rehydration. Detached fronds were used to monitor the rates of photosynthesis in desiccation experiments, sugar accumulation, and high-resolution 2-DE to analyze proteome variation during a desiccation-rehydration cycle. The analyzed proteome exhibited little variation (3-4%) between hydrated and desiccated states, while variation was greater between the desiccated and rehydrated states (8.7-10%). Eighty-two discrete proteins were analyzed by MS/MS, and 65 were identified. About 21% of the analyzed proteins (17) were mixtures of two or more different polypeptides. Of the identified proteins, more than a half (33 spots, 55%) had functions related to energy-photosynthesis. The second largest category with known function (five spots, 8%) was related to cell rescue, defense, and virulence. More than one in every four proteins analyzed belonged to a group of hypothetical proteins (18 spots, 28%). The results suggest that the Hymenophyllaceae represent an example of a change in adaptive strategy from a typical vascular to the poikilohydric homoiochlorophyllous adaptation, which they share with the bryophytes that grow in profusion in the same habitats. The speed at which

  7. Photoprotection of green plants: a mechanism of ultra-fast thermal energy dissipation in desiccated lichens.

    PubMed

    Heber, Ulrich

    2008-09-01

    In order to survive sunlight in the absence of water, desiccation-tolerant green plants need to be protected against photooxidation. During drying of the chlorolichen Cladonia rangiformis and the cyanolichen Peltigera neckeri, chlorophyll fluorescence decreased and stable light-dependent charge separation in reaction centers of the photosynthetic apparatus was lost. The presence of light during desiccation increased loss of fluorescence in the chlorolichen more than that in the cyanolichen. Heating of desiccated Cladonia thalli, but not of Peltigera thalli, increased fluorescence emission more after the lichen had been dried in the light than after drying in darkness. Activation of zeaxanthin-dependent energy dissipation by protonation of the PsbS protein of thylakoid membranes was not responsible for the increased loss of chlorophyll fluorescence by the chlorolichen during drying in the light. Glutaraldehyde inhibited loss of chlorophyll fluorescence during drying. Desiccation-induced loss of chlorophyll fluorescence and of light-dependent charge separation are interpreted to indicate activation of a highly effective mechanism of photoprotection in the lichens. Activation is based on desiccation-induced conformational changes of a pigment-protein complex. Absorbed light energy is converted into heat within a picosecond or femtosecond time domain. When present during desiccation, light interacts with the structural changes of the protein providing increased photoprotection. Energy dissipation is inactivated and structural changes are reversed when water becomes available again. Reversibility of ultra-fast thermal dissipation of light energy avoids photo-damage in the absence of water and facilitates the use of light for photosynthesis almost as soon as water becomes available.

  8. A simple analytical method to estimate all exit parameters of a cross-flow air dehumidifier using liquid desiccant.

    PubMed

    Bassuoni, M M

    2014-03-01

    The dehumidifier is a key component in liquid desiccant air-conditioning systems. Analytical solutions have more advantages than numerical solutions in studying the dehumidifier performance parameters. This paper presents the performance results of exit parameters from an analytical model of an adiabatic cross-flow liquid desiccant air dehumidifier. Calcium chloride is used as desiccant material in this investigation. A program performing the analytical solution is developed using the engineering equation solver software. Good accuracy has been found between analytical solution and reliable experimental results with a maximum deviation of +6.63% and -5.65% in the moisture removal rate. The method developed here can be used in the quick prediction of the dehumidifier performance. The exit parameters from the dehumidifier are evaluated under the effects of variables such as air temperature and humidity, desiccant temperature and concentration, and air to desiccant flow rates. The results show that hot humid air and desiccant concentration have the greatest impact on the performance of the dehumidifier. The moisture removal rate is decreased with increasing both air inlet temperature and desiccant temperature while increases with increasing air to solution mass ratio, inlet desiccant concentration, and inlet air humidity ratio.

  9. A simple analytical method to estimate all exit parameters of a cross-flow air dehumidifier using liquid desiccant

    PubMed Central

    Bassuoni, M.M.

    2013-01-01

    The dehumidifier is a key component in liquid desiccant air-conditioning systems. Analytical solutions have more advantages than numerical solutions in studying the dehumidifier performance parameters. This paper presents the performance results of exit parameters from an analytical model of an adiabatic cross-flow liquid desiccant air dehumidifier. Calcium chloride is used as desiccant material in this investigation. A program performing the analytical solution is developed using the engineering equation solver software. Good accuracy has been found between analytical solution and reliable experimental results with a maximum deviation of +6.63% and −5.65% in the moisture removal rate. The method developed here can be used in the quick prediction of the dehumidifier performance. The exit parameters from the dehumidifier are evaluated under the effects of variables such as air temperature and humidity, desiccant temperature and concentration, and air to desiccant flow rates. The results show that hot humid air and desiccant concentration have the greatest impact on the performance of the dehumidifier. The moisture removal rate is decreased with increasing both air inlet temperature and desiccant temperature while increases with increasing air to solution mass ratio, inlet desiccant concentration, and inlet air humidity ratio. PMID:25685485

  10. Maintenance or Collapse: Responses of Extraplastidic Membrane Lipid Composition to Desiccation in the Resurrection Plant Paraisometrum mileense

    PubMed Central

    Yu, Buzhu; Yu, Xiaomei; Li, Weiqi

    2014-01-01

    Resurrection plants usually grow in specific or extreme habitats and have the capacity to survive almost complete water loss. We characterized the physiological and biochemical responses of Paraisometrum mileense to extreme desiccation and found that it is a resurrection plant. We profiled the changes in lipid molecular species during dehydration and rehydration in P. mileense, and compared these with corresponding changes in the desiccation-sensitive plant Arabidopsis thaliana. One day of desiccation was lethal for A. thaliana but not for P. mileense. After desiccation and subsequent rewatering, A. thaliana showed dramatic lipid degradation accompanied by large increases in levels of phosphatidic acid (PA) and diacylglycerol (DAG). In contrast, desiccation and rewatering of P. mileense significantly decreased the level of monogalactosyldiacylglycerol and increased the unsaturation of membrane lipids, without changing the level of extraplastidic lipids. Lethal desiccation in P. mileense caused massive lipid degradation, whereas the PA content remained at a low level similar to that of fresh leaves. Neither damage nor repair processes, nor increases in PA, occurred during non-lethal desiccation in P. mileense. The activity of phospholipase D, the main source of PA, was much lower in P. mileense than in A. thaliana under control conditions, or after either dehydration or rehydration. It was demonstrated that low rates of phospholipase D-mediated PA formation in P. mileense might limit its ability to degrade lipids to PA, thereby maintaining membrane integrity following desiccation. PMID:25068901

  11. Freezing and Desiccation Tolerance in Entomopathogenic Nematodes: Diversity and Correlation of Traits

    PubMed Central

    Shapiro-Ilan, David I.; Brown, Ian; Lewis, Edwin E.

    2014-01-01

    The ability of entomopathogenic nematodes to tolerate environmental stress such as desiccating or freezing conditions, can contribute significantly to biocontrol efficacy. Thus, in selecting which nematode to use in a particular biocontrol program, it is important to be able to predict which strain or species to use in target areas where environmental stress is expected. Our objectives were to (i) compare inter- and intraspecific variation in freeze and desiccation tolerance among a broad array of entomopathogenic nematodes, and (ii) determine if freeze and desiccation tolerance are correlated. In laboratory studies we compared nematodes at two levels of relative humidity (RH) (97% and 85%) and exposure periods (24 and 48 h), and nematodes were exposed to freezing temperatures (-2°C) for 6 or 24 h. To assess interspecific variation, we compared ten species including seven that are of current or recent commercial interest: Heterorhabditis bacteriophora (VS), H. floridensis, H. georgiana, (Kesha), H. indica (HOM1), H. megidis (UK211), Steinernema carpocapsae (All), S. feltiae (SN), S. glaseri (VS), S. rarum (17C&E), and S. riobrave (355). To assess intraspecific variation we compared five strains of H. bacteriophora (Baine, Fl1-1, Hb, Oswego, and VS) and four strains of S. carpocapsae (All, Cxrd, DD136, and Sal), and S. riobrave (355, 38b, 7-12, and TP). S. carpocapsae exhibited the highest level of desiccation tolerance among species followed by S. feltiae and S. rarum; the heterorhabditid species exhibited the least desiccation tolerance and S. riobrave and S. glaseri were intermediate. No intraspecific variation was observed in desiccation tolerance; S. carpocapsae strains showed higher tolerance than all H. bacteriophora or S. riobrave strains yet there was no difference detected within species. In interspecies comparisons, poor freeze tolerance was observed in H. indica, and S. glaseri, S. rarum, and S. riobrave whereas H. georgiana and S. feltiae exhibited the

  12. Effects of temperature on desiccant catalysis of refrigerant and lubricant decomposition. Final report

    SciTech Connect

    Rohatgi, N.D.T.

    1998-06-01

    Accelerated aging at high temperatures (149 C) for short aging times (28 days) is effective in screening the compatibility of different materials in refrigeration systems. However, in actual applications temperatures are usually lower and operating times much longer. Therefore plots to allow for interpolation or extrapolation of experimental data to actual operating conditions are needed. In the current study, aging of refrigerant/lubricant/desiccant/metal systems was conducted at five different temperatures, and for each temperature at four different aging times. The data collected from this study provided plots relating refrigerant or lubricant decomposition to aging time, aging temperature, and type of desiccant, which can be used for interpolation or extrapolation.

  13. QTLs and candidate genes for desiccation and abscisic acid content in maize kernels

    PubMed Central

    2010-01-01

    Background Kernel moisture at harvest is an important trait since a low value is required to prevent unexpected early germination and ensure seed preservation. It is also well known that early germination occurs in viviparous mutants, which are impaired in abscisic acid (ABA) biosynthesis. To provide some insight into the genetic determinism of kernel desiccation in maize, quantitative trait loci (QTLs) were detected for traits related to kernel moisture and ABA content in both embryo and endosperm during kernel desiccation. In parallel, the expression and mapping of genes involved in kernel desiccation and ABA biosynthesis, were examined to detect candidate genes. Results The use of an intermated recombinant inbred line population allowed for precise QTL mapping. For 29 traits examined in an unreplicated time course trial of days after pollination, a total of 78 QTLs were detected, 43 being related to kernel desiccation, 15 to kernel weight and 20 to ABA content. Multi QTL models explained 35 to 50% of the phenotypic variation for traits related to water status, indicating a large genetic control amenable to breeding. Ten of the 20 loci controlling ABA content colocated with previously detected QTLs controlling water status and ABA content in water stressed leaves. Mapping of candidate genes associated with kernel desiccation and ABA biosynthesis revealed several colocations between genes with putative functions and QTLs. Parallel investigation via RT-PCR experiments showed that the expression patterns of the ABA-responsive Rab17 and Rab28 genes as well as the late embryogenesis abundant Emb5 and aquaporin genes were related to desiccation rate and parental allele effect. Database searches led to the identification and mapping of two zeaxanthin epoxidase (ZEP) and five novel 9-cis-epoxycarotenoid dioxygenase (NCED) related genes, both gene families being involved in ABA biosynthesis. The expression of these genes appeared independent in the embryo and endosperm

  14. Restaurant food cooling practices.

    PubMed

    Brown, Laura Green; Ripley, Danny; Blade, Henry; Reimann, Dave; Everstine, Karen; Nicholas, Dave; Egan, Jessica; Koktavy, Nicole; Quilliam, Daniela N

    2012-12-01

    Improper food cooling practices are a significant cause of foodborne illness, yet little is known about restaurant food cooling practices. This study was conducted to examine food cooling practices in restaurants. Specifically, the study assesses the frequency with which restaurants meet U.S. Food and Drug Administration (FDA) recommendations aimed at reducing pathogen proliferation during food cooling. Members of the Centers for Disease Control and Prevention's Environmental Health Specialists Network collected data on food cooling practices in 420 restaurants. The data collected indicate that many restaurants are not meeting FDA recommendations concerning cooling. Although most restaurant kitchen managers report that they have formal cooling processes (86%) and provide training to food workers on proper cooling (91%), many managers said that they do not have tested and verified cooling processes (39%), do not monitor time or temperature during cooling processes (41%), or do not calibrate thermometers used for monitoring temperatures (15%). Indeed, 86% of managers reported cooling processes that did not incorporate all FDA-recommended components. Additionally, restaurants do not always follow recommendations concerning specific cooling methods, such as refrigerating cooling food at shallow depths, ventilating cooling food, providing open-air space around the tops and sides of cooling food containers, and refraining from stacking cooling food containers on top of each other. Data from this study could be used by food safety programs and the restaurant industry to target training and intervention efforts concerning cooling practices. These efforts should focus on the most frequent poor cooling practices, as identified by this study.

  15. Primary Energy Efficiency Analysis of Different Separate Sensible and Latent Cooling Techniques

    SciTech Connect

    Abdelaziz, Omar

    2015-01-01

    Separate Sensible and Latent cooling (SSLC) has been discussed in open literature as means to improve air conditioning system efficiency. The main benefit of SSLC is that it enables heat source optimization for the different forms of loads, sensible vs. latent, and as such maximizes the cycle efficiency. In this paper I use a thermodynamic analysis tool in order to analyse the performance of various SSLC technologies including: multi-evaporators two stage compression system, vapour compression system with heat activated desiccant dehumidification, and integrated vapour compression with desiccant dehumidification. A primary coefficient of performance is defined and used to judge the performance of the different SSLC technologies at the design conditions. Results showed the trade-off in performance for different sensible heat factor and regeneration temperatures.

  16. Comparative genome sequencing reveals genomic signature of extreme desiccation tolerance in the anhydrobiotic midge

    PubMed Central

    Gusev, Oleg; Suetsugu, Yoshitaka; Cornette, Richard; Kawashima, Takeshi; Logacheva, Maria D.; Kondrashov, Alexey S.; Penin, Aleksey A.; Hatanaka, Rie; Kikuta, Shingo; Shimura, Sachiko; Kanamori, Hiroyuki; Katayose, Yuichi; Matsumoto, Takashi; Shagimardanova, Elena; Alexeev, Dmitry; Govorun, Vadim; Wisecaver, Jennifer; Mikheyev, Alexander; Koyanagi, Ryo; Fujie, Manabu; Nishiyama, Tomoaki; Shigenobu, Shuji; Shibata, Tomoko F.; Golygina, Veronika; Hasebe, Mitsuyasu; Okuda, Takashi; Satoh, Nori; Kikawada, Takahiro

    2014-01-01

    Anhydrobiosis represents an extreme example of tolerance adaptation to water loss, where an organism can survive in an ametabolic state until water returns. Here we report the first comparative analysis examining the genomic background of extreme desiccation tolerance, which is exclusively found in larvae of the only anhydrobiotic insect, Polypedilum vanderplanki. We compare the genomes of P. vanderplanki and a congeneric desiccation-sensitive midge P. nubifer. We determine that the genome of the anhydrobiotic species specifically contains clusters of multi-copy genes with products that act as molecular shields. In addition, the genome possesses several groups of genes with high similarity to known protective proteins. However, these genes are located in distinct paralogous clusters in the genome apart from the classical orthologues of the corresponding genes shared by both chironomids and other insects. The transcripts of these clustered paralogues contribute to a large majority of the mRNA pool in the desiccating larvae and most likely define successful anhydrobiosis. Comparison of expression patterns of orthologues between two chironomid species provides evidence for the existence of desiccation-specific gene expression systems in P. vanderplanki. PMID:25216354

  17. Preharvest herbicide treatments affect black bean desiccation, yield, and canned bean color

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A field trial was conducted near Richville, Michigan in 2013 and 2014 to evaluate the effects of preharvest herbicide treatments on desiccation, yield, and canned black bean quality and color. Three Type II black bean varieties, Zorro, Eclipse, and Zenith, were planted on two different dates in each...

  18. Using experimental evolution to study the physiological mechanisms of desiccation resistance in Drosophila melanogaster.

    PubMed

    Archer, Margaret A; Bradley, Timothy J; Mueller, Laurence D; Rose, Michael R

    2007-01-01

    Data from populations undergoing experimental evolution can be used to make comparisons between physiologically differentiated populations and to determine evolutionary trajectories. Comparisons of long-established laboratory populations of Drosophila melanogaster that are strongly differentiated with respect to desiccation resistance are used to test alternative hypotheses concerning the mechanisms that fruit flies use to survive bouts of extreme desiccation. This comparative study supports the hypothesis that, in at least one case, D. melanogaster can evolve increased resistance to desiccation by decreasing water loss rates and by increasing bulk water content but not by increasing metabolic water content or dehydration tolerance. While glycogen was involved in water storage, its primary role was in water binding, not the production of metabolic water. Measurement of the trajectories of these component mechanisms during selection for desiccation resistance is used to demonstrate that water loss rate quickly plateaus in response to selection, while water content continues to improve. This disparity reveals the value of studying evolutionary trajectories and the need for longer-term selection studies in evolutionary physiology.

  19. Thermoluminescence study of photosystem II activity in Haberlea rhodopensis and spinach leaves during desiccation.

    PubMed

    Peeva, V; Maslenkova, L

    2004-05-01

    Thermoluminescence glow curve parameters were used to access the functional features of PS II in the Balkan endemic Haberlea rhodopensis. This representative of the higher desiccation-tolerant plants is unique for the European flora. An unusual high temperature of TL emission from Haberlea leaves after excitation by one flash at 5 degrees C was observed. The position of the main TL B band (S (2)Q (B)(-)) was at 45 - 47 degrees C, while this temperature was 30 - 32 degrees C in drought-sensitive mesophytic spinach. Consistent with the up-shift in TL emission, the lifetime of the S (2) state was also increased, showing a stabilization of charge storage in PS II complex in this resurrection plant. In addition, a part of PS II centres was less susceptible to DCMU. We consider the observed unusual TL characteristics of Haberlea rhodopensis reflect some structural modifications in PS II (especially in D1 protein), which could be related to the desiccation tolerance of this plant. This suggestion was supported by the different manner in which dehydration affected the TL properties in desiccation-tolerant Haberlea and desiccation-sensitive spinach plants.

  20. Osmotic damage as a predictor of motility loss during convective desiccation of bovine sperm.

    PubMed

    Sitaula, Ranjan; Jimenez, Jorge; Bhowmick, Sankha

    2013-12-01

    Current state-of-the art technologies are lagging in the application of desiccation storage to mammalian cells using nonreducing sugars. For bovine sperm, motility is irreversibly lost before reaching a sufficiently low moisture content necessary for preservation. It is hypothesized that much of the damage during drying is related to the osmotic stress encountered due to increased osmolarity of the extracellular environment. To test this hypothesis, we subjected sperm to liquid hyperosmotic environments for varying time-periods and measured their motility. We then extracted parameters for two models for motility loss based on these experiments: a first-order rate injury model (Fast or Slow) and a multi-modal (MM) injury model. The MM injury model incorporated an additional function accounting for damage induced by a time-independent osmotic change. Based on these models, we predicted sperm motility loss measured from natural and forced convective desiccation experiments. The MM injury model was able to closely bracket motility loss for desiccation as an osmotic change event with time-independent and time-dependent components. While the mechanistic basis of osmotic damage requires further exploration, the model can serve as a bracketing tool for predicting motility loss during desiccation based on excipients designed to minimize osmotic damage.

  1. Desiccation increases the efficacy of Beauveria bassiana for stored grain pest insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effect of desiccation stress on the efficacy of Beauveria bassiana for stored-product insects was investigated in laboratory bioassays. The mortality of B. bassiana-treated Plodia interpunctella larvae was greater at a vapor pressure deficit (VPD) of 2.42 kPa or 1.87 kPa than at 1.06 kPa. Moist...

  2. Toward an index of desiccation times to tree mortality under drought

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Research in plant hydraulics has provided important insights into plant responses to drought and species absolute drought tolerance. However our ability to predict when plants will die under extreme drought may be limited by a lack of knowledge with regards to the dynamics of plant desiccation from ...

  3. Giant desiccation fissures on the Black Rock and Smoke Creek Deserts, Nevada

    USGS Publications Warehouse

    Willden, R.; Mabey, D.R.

    1961-01-01

    Open fissures, from 100 to several hundred feet apart, that have produced polygonal patterns on the Black Rock Desert, Nevada, are believed to be giant desiccation cracks resulting from a secular trend toward aridity in the last few decades. Similar features on the Smoke Creek Desert probably have the same origin.

  4. The impact of desiccation on the adhesion of barnacles attached to non-stick coatings.

    PubMed

    Wiegemann, Maja; Watermann, Burkard

    2004-06-01

    Fouling-release coatings prevent fouling of ships' hulls through hydrodynamic forces generated as the ship moves through the water. The effectiveness of such coatings may be evaluated by measuring the adhesion strength of settled organisms, e.g. barnacles. The influence of desiccation of the barnacle adhesive on such measurements was investigated. Shear forces required to remove barnacles of the genus Balanus increased during the course of desiccation up to the point when the barnacles suddenly self-detached. The increase was thought to be due to the rising cohesive strength of the adhesive. Growing tensile forces within the weakly cross-linked adhesive, however, are suggested to have led to self-detachment. The shear forces required to remove barnacles of the genus Elminius were generally low and did not differ significantly during the course of desiccation. The different results may be attributed to specific base morphologies. It was concluded that measuring the adhesion strength of members of the Balanidae on non-stick surfaces in air could produce flawed results due to the influence of desiccation of the barnacle adhesive. The investigations have also provided new insights into the characteristics of barnacle adhesive.

  5. Some thoughts about the desiccation tolerance continuum and the basis for “intermediate” seed physiologies

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Desiccation-sensitive seeds succumb when dried below a critical water content. Critical water contents range widely among species and with experimental conditions, implying a continuum in the amount of water that can be non-lethally removed from diverse cells. However, water potentials correspondi...

  6. Sensor and numerical simulator evaluation for porous medium desiccation and rewetting at the intermediate laboratory scale

    SciTech Connect

    Oostrom, Martinus; Wietsma, Thomas W.; Strickland, Christopher E.; Freedman, Vicky L.; Truex, Michael J.

    2012-02-01

    Soil desiccation, in conjunction with surface infiltration control, is considered at the Hanford Site as a potential technology to limit the flux of technetium and other contaminants in the vadose zone to the groundwater. An intermediate-scale experiment was conducted to test the response of a series of instruments to desiccation and subsequent rewetting of porous media. The instruments include thermistors, thermocouple psychrometers, dual-probe heat pulse sensors, heat dissipation units, and humidity probes. The experiment was simulated with the multifluid flow simulator STOMP, using independently obtained hydraulic and thermal porous medium properties. All instrument types used for this experiment were able to indicate when the desiccation front passed a certain location. In most cases the changes were sharp, indicating rapid changes in moisture content, water potential, or humidity. However, a response to the changing conditions was recorded only when the drying front was very close to a sensor. Of the tested instruments, only the heat dissipation unit and humidity probes were able to detect rewetting. The numerical simulation results reasonably match the experimental data, indicating that the simulator captures the pertinent gas flow and transport processes related to desiccation and rewetting and may be useful in the design and analysis of field tests.

  7. Desiccation tolerance of Botryococcus braunii (Trebouxiophyceae, Chlorophyta) and extreme temperature tolerance of dehydrated cells.

    PubMed

    Demura, Mikihide; Ioki, Motohide; Kawachi, Masanobu; Nakajima, Nobuyoshi; Watanabe, Makoto M

    2014-01-01

    Botryococcus braunii Kützing, a green colonial microalga, occurs worldwide in both freshwater and brackish water environments. Despite considerable attention to B. braunii as a potential source of renewable fuel, many ecophysiological properties of this alga remain unknown. Here, we examined the desiccation and temperature tolerances of B. braunii using two newly isolated strains BOD-NG17 and BOD-GJ2. Both strains survived through 6- and 8-month desiccation treatments but not through a 12-month treatment. Interestingly, the desiccation-treated cells of B. braunii gained tolerance to extreme temperature shifts, i.e., high temperature (40 °C) and freezing (-20 °C). Both strains survived for at least 4 and 10 days at 40 and -20 °C, respectively, while the untreated cells barely survived at these temperatures. These traits would enable long-distance dispersal of B. braunii cells and may account for the worldwide distribution of this algal species. Extracellular substances such as polysaccharides and hydrocarbons seem to confer the desiccation tolerance. PMID:24600162

  8. Molecular approaches for improving desiccation tolerance: Insights from the brine shrimp Artemia franciscana

    PubMed Central

    Hand, Steven C.; Menze, Michael A.

    2015-01-01

    Organisms inhabiting both aquatic and terrestrial ecosystems frequently are confronted with the problem of water loss for multiple reasons – exposure to hypersalinity, evaporative water loss, and restriction of intracellular water due to freezing of extracellular fluids. Seasonal desiccation can become severe and lead to the production of tolerant propagules and entry into the state of anhydrobiosis at various stages of the life cycle. Such is the case for gastrula-stage embryos of the brine shrimp, Artemia franciscana. Physiological and biochemical responses to desiccation are central for survival and are multifaceted. This review will evaluate the impact of multiple Late Embryogenesis Abundant (LEA) proteins originating from A. franciscana, together with the non-reducing sugar trehalose, on prevention of desiccation damage at multiple levels of biological organization. Survivorship of desiccation-sensitive cells during water stress can be improved by use of the above protective agents, coupled to metabolic preconditioning and rapid cell drying. However, obtaining long-term stability of cells in the dried state at room temperature has not been accomplished and will require continued efforts on both the physicochemical and biological fronts. PMID:25809151

  9. Understanding Vegetative Desiccation Tolerance using Integrated Functional Genomics Approaches within a Comparative Evolutionary Framework

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Desiccation tolerance (DT) is defined as the equilibration of protoplasmic water potential with that of the surrounding air (generally dry) without loss of viability upon rehydration. Vegetative DT is widespread amongst mosses and lichens, but is relatively rare in vascular plants (0.15%). Recent st...

  10. The response of Asterochloris erici (Ahmadjian) Skaloud et Peksa to desiccation: a proteomic approach

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The study of desiccation tolerance of lichens, and of their photobionts in particular, has frequently focused on the antioxidant system that protects the cell against photo-oxidative stress during dehydration/rehydration cycles. Thus, in this work we carried out proteomic and transcript analyses of ...

  11. Ecological and evolutionary consequences of desiccation tolerance in tropical fern gametophytes.

    PubMed

    Watkins, James E; Mack, Michelle C; Sinclair, Thomas R; Mulkey, Stephen S

    2007-01-01

    Ferns have radiated into the same diverse environments as spermatophytes, and have done so with an independent gametophyte that is not protected by the parent plant. The degree and extent of desiccation tolerance (DT) in the gametophytes of tropical fern species was assessed to understand mechanisms that have allowed ferns to radiate into a diversity of habitats. Species from several functional groups were subjected to a series of desiccation events, including varying degrees of intensity and multiple desiccation cycles. Measurements of chlorophyll fluorescence were used to assess recovery ability and compared with species ecology and gametophyte morphology. It is shown that vegetative DT (rare in vascular plants) is widely exhibited in fern gametophytes and the degree of tolerance is linked to species habitat preference. It is proposed that gametophyte morphology influences water-holding capacity, a novel mechanism that may help to explain how ferns have radiated into drought-prone habitats. Fern gametophytes have often been portrayed as extreme mesophytes with little tolerance for desiccation. The discovery of DT in gametophytes holds potential for improving our understanding of both the controls on fern species distribution and their evolution. It also advances a new system with which to study the evolution of DT in vascular plants.

  12. C4 grasses prosper as carbon dioxide eliminates desiccation in warmed semi-arid grasslands

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Global warming is predicted to induce desiccation in many world regions through increases in evaporative demand. Rising CO2 may counter that trend by improving plant water use efficiency (WUE). However, it is not clear how important this CO2-enhanced WUE might be in off-setting warming-induced desi...

  13. A new microscopic method to analyse desiccation-induced volume changes in aeroterrestrial green algae.

    PubMed

    Lajos, K; Mayr, S; Buchner, O; Blaas, K; Holzinger, A

    2016-08-01

    Aeroterrestrial green algae are exposed to desiccation in their natural habitat, but their actual volume changes have not been investigated. Here, we measure the relative volume reduction (RVRED ) in Klebsormidium crenulatum and Zygnema sp. under different preset relative air humidities (RH). A new chamber allows monitoring RH during light microscopic observation of the desiccation process. The RHs were set in the range of ∼4 % to ∼95% in 10 steps. RVRED caused by the desiccation process was determined after full acclimation to the respective RHs. In K. crenulatum, RVRED (mean ± SE) was 46.4 ± 1.9%, in Zygnema sp. RVRED was only 34.3 ± 2.4% at the highest RH (∼95%) tested. This indicates a more pronounced water loss at higher RHs in K. crenulatum versus Zygnema sp. By contrast, at the lowest RH (∼4%) tested, RVRED ranged from 75.9 ± 2.7% in K. crenulatum to 83.9 ± 2.2% in Zygnema sp. The final volume reduction is therefore more drastic in Zygnema sp. These data contribute to our understanding of the desiccation process in streptophytic green algae, which are considered the closest ancestors of land plants. PMID:27075881

  14. Exploring the role of trehalose metabolism in resistance to oxidative and desiccation stress in Fusarium verticillioides

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fusarium verticillioides is a pathogenic filamentous fungus that primarily affects maize. We are exploring stress response mechanisms in F. verticillioides, particularly the role of trehalose, a disaccharide known to be involved in the ability of several organisms to withstand desiccation or drought...

  15. Desiccation sensitivity and tolerance in the moss Physcomitrella patens: assessing limits and damage.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The moss Physcomitrella patens is becoming the model of choice for functional genomic studies at the cellular level. Studies report that P. patens survives moderate osmotic and salt stress, and that desiccation tolerance can be induced by exogenous ABA. Our goal was to quantify the extent of dehydr...

  16. Biofilm resilience to desiccation in groundwater aquifers: a laboratory and field study.

    PubMed

    Weaver, L; Webber, J B; Hickson, A C; Abraham, P M; Close, M E

    2015-05-01

    Groundwater is used as a precious resource for drinking water worldwide. Increasing anthropogenic activity is putting increasing pressure on groundwater resources. One impact of increased groundwater abstraction coupled with increasing dry weather events is the lowering of groundwater levels within aquifers. Biofilms within groundwater aquifers offer protection to the groundwater by removing contaminants entering the aquifer systems from land use activities. The study presented investigated the impact of desiccation events on the biofilms present in groundwater aquifers using field and laboratory experiments. In both field and laboratory experiments a reduction in enzyme activity (glucosidase, esterase and phosphatase) was seen during desiccation compared to wet controls. However, comparing all the data together no significant differences were seen between either wet or desiccated samples or between the start and end of the experiments. In both field and laboratory experiments enzyme activity recovered to start levels after return to wet conditions. The study shows that biofilms within groundwater systems are resilient and can withstand periods of desiccation (4 months).

  17. Food availability determines the response to pond desiccation in anuran tadpoles.

    PubMed

    Enriquez-Urzelai, Urtzi; San Sebastián, Olatz; Garriga, Núria; Llorente, Gustavo A

    2013-09-01

    Food availability and pond desiccation are two of the most studied factors that condition amphibian metamorphosis. It is well known that, when food is abundant, organisms undergo metamorphosis early and when they are relatively large. The capability of anurans to accelerate their developmental rate in response to desiccation is also common knowledge. These two variables must act together in nature, since we know that, as a pond dries, the per capita resources decrease. We conduct an experiment to evaluate the effects of desiccation and food availability separately and in combination in tadpoles of the painted frog (Discoglossus pictus). We demonstrate that food deprivation leads to slow growth rates, which delay metamorphosis and produce smaller size and weight. The capability to accelerate metamorphosis when facing a drying pond is also confirmed, but, nevertheless, with factor interaction (when the pool is drying and resources are scarce) the capacity to respond to desiccation is lost. In addition, slow drying rates are shown to be stressful situations, but not enough to provoke a shortening of the larval period; in fact, the larval period becomes longer. We also demonstrate that the interaction of these factors changes the allometric relationship of different parts of the hind limb, which has implications for the biomechanics of jumping. Due to low mortality rates and an adequate response to both environmental factors, we expect D. pictus to have a great invasive potential in its new Mediterranean distribution area, where lots of temporary and ephemeral ponds are present.

  18. Loss of desiccation tolerance in Copaifera langsdorffii Desf. seeds during germination.

    PubMed

    Pereira, W V S; Faria, J M R; Tonetti, O A O; Silva, E A A

    2014-05-01

    This study evaluated the loss of desiccation tolerance in C. langsdorffii seeds during the germination process. Seeds were imbibed for 24, 48, 72, 96, 120 and 144 hours and dried to the initial moisture content, kept in this state for 3 days after which they were submitted to pre-humidification and rehydration. Ultraestructural evaluations were done aiming to observe the cell damage caused by the dry process. Desiccation tolerance was evaluated in terms of the percentage of normal seedlings. Seeds not submitted to the drying process presented 61% of normal seedlings, and after 24 hours of imbibition, followed by drying, the seeds presented the same percentage of survival. However, after 48 hours of imbibition, seeds started to lose the desiccation tolerance. There was twenty six percent of normal seedlings formed from seeds imbibed for 96 hours and later dried and rehydrated. Only 5% of seeds imbibed for 144 hours, dried and rehydrated formed normal seedlings. At 144 hours of imbibition followed the dry process, there was damage into the cell structure, indicating that the seeds were unable to keep the cell structure during the drying process. Copaifera langsdorffii seeds loses the desiccation tolerance at the start of Phase 2 of imbibition.

  19. Abscisic acid (ABA) sensitivity regulates desiccation tolerance in germinated Arabidopsis seeds.

    PubMed

    Maia, Julio; Dekkers, Bas J W; Dolle, Miranda J; Ligterink, Wilco; Hilhorst, Henk W M

    2014-07-01

    During germination, orthodox seeds lose their desiccation tolerance (DT) and become sensitive to extreme drying. Yet, DT can be rescued, in a well-defined developmental window, by the application of a mild osmotic stress before dehydration. A role for abscisic acid (ABA) has been implicated in this stress response and in DT re-establishment. However, the path from the sensing of an osmotic cue and its signaling to DT re-establishment is still largely unknown. Analyses of DT, ABA sensitivity, ABA content and gene expression were performed in desiccation-sensitive (DS) and desiccation-tolerant Arabidopsis thaliana seeds. Furthermore, loss and re-establishment of DT in germinated Arabidopsis seeds was studied in ABA-deficient and ABA-insensitive mutants. We demonstrate that the developmental window in which DT can be re-established correlates strongly with the window in which ABA sensitivity is still present. Using ABA biosynthesis and signaling mutants, we show that this hormone plays a key role in DT re-establishment. Surprisingly, re-establishment of DT depends on the modulation of ABA sensitivity rather than enhanced ABA content. In addition, the evaluation of several ABA-insensitive mutants, which can still produce normal desiccation-tolerant seeds, but are impaired in the re-establishment of DT, shows that the acquisition of DT during seed development is genetically different from its re-establishment during germination.

  20. Hybrid radiator cooling system

    DOEpatents

    France, David M.; Smith, David S.; Yu, Wenhua; Routbort, Jules L.

    2016-03-15

    A method and hybrid radiator-cooling apparatus for implementing enhanced radiator-cooling are provided. The hybrid radiator-cooling apparatus includes an air-side finned surface for air cooling; an elongated vertically extending surface extending outwardly from the air-side finned surface on a downstream air-side of the hybrid radiator; and a water supply for selectively providing evaporative cooling with water flow by gravity on the elongated vertically extending surface.

  1. Sorption and desorption characteristics of a packed bed of clay-CaCl{sub 2} desiccant particles

    SciTech Connect

    Tretiak, C.S.; Abdallah, N. Ben

    2009-10-15

    Desiccants can be used in conjunction with solar energy to provide a viable alternative to traditional air conditioning techniques. A desiccant consisting of clay and calcium chloride was developed and tested using multiple sorption and desorption cycles. During sorption, inlet air temperatures from 23 to 36 C with corresponding relative humidities of 42-66% were tested. Additionally, superficial air velocities from 0.17 to 0.85 m/s were tested. During desorption, inlet air temperatures from 50 to 57 C and superficial air velocities of approximately 0.30 and 0.60 m/s were tested. A regression equation was determined for the mass of water sorbed by the clay-CaCl2 desiccant with a R{sup 2} value of 0.917. The desorption data was regressed to an exponential function and significant k-values were determined. An equation for pressure drop through the desiccant was determined and compared to existing models. The desiccant was found to perform well during the repeated test cycles though small masses of desiccant were lost due to surface disintegration of the desiccant spheres. (author)

  2. Gateway to genetic exchange? DNA double-strand breaks in the bdelloid rotifer Adineta vaga submitted to desiccation.

    PubMed

    Hespeels, B; Knapen, M; Hanot-Mambres, D; Heuskin, A-C; Pineux, F; LUCAS, S; Koszul, R; Van Doninck, K

    2014-07-01

    The bdelloid rotifer lineage Adineta vaga inhabits temporary habitats subjected to frequent episodes of drought. The recently published draft sequence of the genome of A. vaga revealed a peculiar genomic structure incompatible with meiosis and suggesting that DNA damage induced by desiccation may have reshaped the genomic structure of these organisms. However, the causative link between DNA damage and desiccation has never been proven to date in rotifers. To test for the hypothesis that desiccation induces DNA double-strand breaks (DSBs), we developed a protocol allowing a high survival rate of desiccated A. vaga. Using pulsed-field gel electrophoresis to monitor genomic integrity, we followed the occurrence of DSBs in dried bdelloids and observed an accumulation of these breaks with time spent in dehydrated state. These DSBs are gradually repaired upon rehydration. Even when the genome was entirely shattered into small DNA fragments by proton radiation, A. vaga individuals were able to efficiently recover from desiccation and repair a large amount of DSBs. Interestingly, when investigating the influence of UV-A and UV-B exposure on the genomic integrity of desiccated bdelloids, we observed that these natural radiations also caused important DNA DSBs, suggesting that the genome is not protected during the desiccated stage but that the repair mechanisms are extremely efficient in these intriguing organisms.

  3. Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation

    PubMed Central

    Bai, Xuegui; Yang, Liming; Tian, Meihua; Chen, Jinhui; Shi, Jisen; Yang, Yongping; Hu, Xiangyang

    2011-01-01

    The viability of recalcitrant seeds is lost following stress from either drying or freezing. Reactive oxygen species (ROS) resulting from uncontrolled metabolic activity are likely responsible for seed sensitivity to drying. Nitric oxide (NO) and the ascorbate-glutathione cycle can be used for the detoxification of ROS, but their roles in the seed response to desiccation remain poorly understood. Here, we report that desiccation induces rapid accumulation of H2O2, which blocks recalcitrant Antiaris toxicaria seed germination; however, pretreatment with NO increases the activity of antioxidant ascorbate-glutathione pathway enzymes and metabolites, diminishes H2O2 production and assuages the inhibitory effects of desiccation on seed germination. Desiccation increases the protein carbonylation levels and reduces protein S-nitrosylation of these antioxidant enzymes; these effects can be reversed with NO treatment. Antioxidant protein S-nitrosylation levels can be further increased by the application of S-nitrosoglutathione reductase inhibitors, which further enhances NO-induced seed germination rates after desiccation and reduces desiccation-induced H2O2 accumulation. These findings suggest that NO reinforces recalcitrant seed desiccation tolerance by regulating antioxidant enzyme activities to stabilize H2O2 accumulation at an appropriate concentration. During this process, protein carbonylation and S-nitrosylation patterns are used as a specific molecular switch to control antioxidant enzyme activities. PMID:21674063

  4. The role of recovery of mitochondrial structure and function in desiccation tolerance of pea seeds.

    PubMed

    Wang, Wei-Qing; Cheng, Hong-Yan; Møller, Ian M; Song, Song-Quan

    2012-01-01

    Mitochondrial repair is of fundamental importance for seed germination. When mature orthodox seeds are imbibed and germinated, they lose their desiccation tolerance in parallel. To gain a better understanding of this process, we studied the recovery of mitochondrial structure and function in pea (Pisum sativum cv. Jizhuang) seeds with different tolerance to desiccation. Mitochondria were isolated and purified from the embryo axes of control and imbibed-dehydrated pea seeds after (re-)imbibition for various times. Recovery of mitochondrial structure and function occurred both in control and imbibed-dehydrated seed embryo axes, but at different rates and to different maximum levels. The integrity of the outer mitochondrial membrane reached 96% in all treatments. However, only the seeds imbibed for 12 h and then dehydrated recovered the integrity of the inner mitochondrial membrane (IMM) and State 3 (respiratory state in which substrate and ADP are present) respiration (with NADH and succinate as substrate) to the control level after re-imbibition. With increasing imbibition time, the degree to which each parameter recovered decreased in parallel with the decrease in desiccation tolerance. The tolerance of imbibed seeds to desiccation increased and decreased when imbibed in CaCl(2) and methylviologen solution, respectively, and the recovery of the IMM integrity similarly improved and weakened in these two treatments, respectively. Survival of seeds after imbibition-dehydration linearly increased with the increase in ability to recover the integrity of IMM and State 3 respiration, which indicates that recovery of mitochondrial structure and function during germination has an important role in seed desiccation tolerance.

  5. Trehalose as an indicator of desiccation stress in Drosophila melanogaster larvae: A potential marker of anhydrobiosis

    SciTech Connect

    Thorat, Leena J.; Gaikwad, Sushama M.; Nath, Bimalendu B.

    2012-03-23

    Highlights: Black-Right-Pointing-Pointer First report confirming anhydrobiosis in Drosophila melanogaster larvae. Black-Right-Pointing-Pointer Trehalose synthesis and accumulation in larvae that hydrolyzed on rehydration. Black-Right-Pointing-Pointer Trehalose synthesis in concert with the enzymes involved in trehalose metabolism. Black-Right-Pointing-Pointer Inhibition of trehalose hydrolysis in presence of a specific trehalase inhibitor. Black-Right-Pointing-Pointer Trehalose proposed as a reliable marker for biomonitoring of climate change studies. -- Abstract: In the current scenario of global climate change, desiccation is considered as one of the major environmental stressors for the biota exposed to altered levels of ambient temperature and humidity. Drosophila melanogaster, a cosmopolitan terrestrial insect has been chosen as a humidity-sensitive bioindicator model for the present study since its habitat undergoes frequent stochastic and/or seasonally aggravated dehydration regimes. We report here for the first time the occurrence of anhydrobiosis in D. melanogaster larvae by subjecting them to desiccation stress under laboratory conditions. Larvae desiccated for ten hours at <5% relative humidity could enter anhydrobiosis and could revive upon rehydration followed by resumption of active metabolism. As revealed by FTIR and HPLC analyzes, our findings strongly indicated the synthesis and accumulation of trehalose in the desiccating larvae. Biochemical measurements pointed out the desiccation-responsive trehalose metabolic pathway that was found to be coordinated in concert with the enzymes trehalose 6-phosphate synthase and trehalase. Further, an inhibitor-based experimental approach using deoxynojirimycin, a specific trehalase inhibitor, demonstrated the pivotal role of trehalose in larval anhydrobiosis of D. melanogaster. We therefore propose trehalose as a potential marker for the assessment of anhydrobiosis in Drosophila. The present findings thus add

  6. Chemical and morphological changes in archaeological seeds and fruits during preservation by desiccation

    NASA Astrophysics Data System (ADS)

    Van Bergen, P. F.; Bland, H. A.; Horton, M. C.; Evershed, R. P.

    1997-05-01

    Soluble and insoluble constituents of modern and ancient (ca. 600 AD) desiccated barley kernels and radish seeds have been studied using high-temperature gas chromatography-mass spectrometry, scanning electron microscopy, and Curie-point pyrolysis-gas chromatography-mass spectrometry. Although some changes in colour are seen, the morphology and anatomy of the desiccated specimens are largely unchanged compared with their extant counterparts. The main chemical alterations arising through long-term desiccation are chemically rather than microbially mediated. Comparison between the lipid data from the modern and ancient barley and radish reveals that extensive ester hydrolysis has occurred over time, while oxidation has been retarded. The insoluble material of the modern barley kernel walls, which is composed of a lignin-cellulose complex characteristic of monocotyledons, undergoes upon desiccation chemical alterations resulting in a significant decrease in the abundance of polysaccharides and cinnamic acids moieties. In marked contrast, the insoluble constituents of the modern radish seed coat yields primarily amino acid moieties upon pyrolysis, most likely deriving from proteins. The seed coal also contains a polyphenolic macromolecule and a small contribution from a dicotyledon lignincellulose complex. This is the first time such a distinct chemical composition has been reported for modern sclerotic plant tissues. The chemical composition of the tissues of the ancient radish specimens appears little altered compared with their modern counterparts; the only obvious difference is the decrease in abundance of 2,6-dimethoxyphenol moieties in the lignin-cellulose pyrolysis products. Comparison of the microscopic and chemical data with that of walls of propagules, i.e., fruits and seeds, deposited in aquatic environments reveals no differences between the material deposited under desiccating and aquatic conditions.

  7. Determination of dimethyl fumarate and other potential allergens in desiccant and antimould sachets.

    PubMed

    Lamas, J Pablo; Sanchez-Prado, Lucia; Regueiro, Jorge; Llompart, Maria; Garcia-Jares, Carmen

    2009-08-01

    A method for the determination of dimethyl fumarate (DMF), benzothiazole (BT) and tert-butylphenol (TBP) in desiccant and antimould agents employed for protecting consumer products from humidity and mould has been developed. The method is based on ultrasound-assisted extraction (UAE) followed by GC-MS analysis. Parameters that could affect the extraction of the compounds have been optimised using a multivariate approach. In the final conditions, the extraction is performed using only 0.5 or 1 mL ethyl acetate and applying ultrasound energy for 5 min. Simultaneous extractions could also be carried out in 5 min without losing efficiency. The method was validated showing good linearity (R2 >0.995). Both intra- and inter-day precisions were studied at several concentration levels, being satisfactory in all cases (RSD <10%). Recovery was evaluated in four real desiccant samples at different compound concentrations, ranging between 87% and 109%. Limits of detection and quantification were in the low nanogramme per gramme level, thus allowing the determination of DMF at concentrations well below the limit established by the recent EU Directive (0.1 microg/g). The proposed procedure was applied to the determination of the target compounds in several desiccant and antimould samples. Although most of them were simply labelled as "silica gel", more than 70% of the tested samples contained high amounts of DMF, many of them at the high microgram per gramme level. Many samples also showed the presence of the other two potential allergens. These results demonstrate that the content of the "desiccant" sachets and tablets in consumer products does not usually belong with the label of the desiccant, and hence, the high risk of exposition to the powerful allergen DMF and other potentially harmful chemicals through consumer goods should be a matter of concern.

  8. Desiccation sensitivity and cryopreservation of excised embryonic axes of Citrus suhuiensis cv. limau madu, Citrumelo [Citrus paradisi macf. × Poncirus trifoliata (l.) raf.] and Fortunella polyandra.

    PubMed

    Al Zoubi, O M; Normah, M N

    2012-01-01

    Excised embryonic axes from seeds of three taxa, namely, Citrus suhuiensis cv. limau madu, Citrumelo (Citrus paradisi x Poncirus trifoliate) and Fortunella polyandra, were desiccated in a laminar airflow, over silica gel, and ultra-rapidly. Desiccation sensitivity (WC50) was estimated for each taxon using the quantal response model. High desiccation tolerance (WC50 = 0.11 g water per g dry mass. g/gdw) was observed for limau madu embryonic axes desiccated in a laminar airflow and ultra-rapidly (WC50 =0.10 g/gdw). Desiccation tolerance was substantially lower (WC50 = 0.19 g/gdw) for silica gel dehydration. Similarly, high desiccation tolerance (WC50 = 0.15 g/gdw) was associated with F. polyandra embryonic axes when desiccated in a laminar airflow, while a lower desiccation tolerance (WC50 = 0.17 g/gdw) was observed with silica gel dehydration. Ultra-rapid desiccation led to the highest desiccation tolerance (WC50 = 0.14 g/gdw). The dehydration rate, however, had no influence on desiccation tolerance (WC50 ~ 0.14 g/gdw) for Citrumelo embryonic axes. After each desiccation period, embryonic axes were directly immersed in liquid nitrogen (LN) followed by rapid rewarming. Normal seedling recovery of 80 to 83% for excised embryonic axes of limau madu was observed for laminar airflow and ultra-rapid dehydration, but for silica gel dehydration, 57% recovery was obtained. Similarly, for Citrumelo, high recoveries of 100% and 97% were obtained from axes desiccated in a laminar airflow and using ultra-rapid dehydration, respectively, whereas a lower value was associated with silica gel dehydration (80%). For F. polyandra, 50% recovery was obtained both for laminar airflow and ultra-rapid dehydration, while much lower recovery (43%) was associated with silica gel dehydration. Regardless of the drying method employed, axis survival percentages following exposure to LN were commensurate with the desiccation sensitivity pattern.

  9. Restaurant food cooling practices.

    PubMed

    Brown, Laura Green; Ripley, Danny; Blade, Henry; Reimann, Dave; Everstine, Karen; Nicholas, Dave; Egan, Jessica; Koktavy, Nicole; Quilliam, Daniela N

    2012-12-01

    Improper food cooling practices are a significant cause of foodborne illness, yet little is known about restaurant food cooling practices. This study was conducted to examine food cooling practices in restaurants. Specifically, the study assesses the frequency with which restaurants meet U.S. Food and Drug Administration (FDA) recommendations aimed at reducing pathogen proliferation during food cooling. Members of the Centers for Disease Control and Prevention's Environmental Health Specialists Network collected data on food cooling practices in 420 restaurants. The data collected indicate that many restaurants are not meeting FDA recommendations concerning cooling. Although most restaurant kitchen managers report that they have formal cooling processes (86%) and provide training to food workers on proper cooling (91%), many managers said that they do not have tested and verified cooling processes (39%), do not monitor time or temperature during cooling processes (41%), or do not calibrate thermometers used for monitoring temperatures (15%). Indeed, 86% of managers reported cooling processes that did not incorporate all FDA-recommended components. Additionally, restaurants do not always follow recommendations concerning specific cooling methods, such as refrigerating cooling food at shallow depths, ventilating cooling food, providing open-air space around the tops and sides of cooling food containers, and refraining from stacking cooling food containers on top of each other. Data from this study could be used by food safety programs and the restaurant industry to target training and intervention efforts concerning cooling practices. These efforts should focus on the most frequent poor cooling practices, as identified by this study. PMID:23212014

  10. Response of the methanogenic microbial communities in Amazonian oxbow lake sediments to desiccation stress.

    PubMed

    Conrad, Ralf; Ji, Yang; Noll, Matthias; Klose, Melanie; Claus, Peter; Enrich-Prast, Alex

    2014-06-01

    Methanogenic microbial communities in soil and sediment function only when the environment is inundated and anoxic. In contrast to submerged soils, desiccation of lake sediments happens only rarely. However, some predictions suggest that extreme events of drying will become more common in the Amazon region, and this will promote an increase in sediments drying and exposure. We asked whether and how such methanogenic communities can withstand desiccation stress. Therefore, we determined the rates and pathways of CH(4) production (analysis of CH(4) and δ(13) C of CH(4), CO(2) and acetate), the copy numbers of bacterial and archaeal 16S rRNA genes and mcrA genes (quantitative PCR), and the community composition of Archaea and Bacteria (T-RFLP and pyrosequencing) in oxbow lake sediments of rivers in the Brazilian Amazon region. The rivers were of white water, black water and clear water type. The measurements were done with sediment in fresh state and after drying and rewetting. After desiccation and rewetting the composition of both, the archaeal and bacterial community changed. Since lake sediments from white water rivers exhibited only negligible methanogenic activity, probably because of relatively high iron and low organic matter content, they were not further analysed. The other sediments produced CH(4), with hydrogenotrophic methanogenesis usually accounting for > 50% of total activity. After desiccation and rewetting, archaeal and bacterial gene copy numbers decreased. The bacterial community showed a remarkable increase of Clostridiales from about 10% to > 30% of all Bacteria, partially caused by proliferation of specific taxa as the numbers of OTU shared with fresh sediment decreased from about 9% to 3%. Among the Archaea, desiccation specifically enhanced the relative abundance of either Methanocellales (black water) and/or Methanosarcinaceae (clear water). Despite the changes in gene copy numbers and composition of the microbial community, rates of CH

  11. Laboratory Colonization, Life History Observations, and Desiccation Tolerance of the Canyon Fly Fannia conspicua (Diptera: Fanniidae).

    PubMed

    Zhang, Congcong; Gerry, Alec C

    2015-07-01

    "Canyon flies" are flies within the Fannia benjamini Malloch species complex. These flies can be considerable pests to humans and animals due to their habit of feeding on animal body secretions such as tears, mucus, sweat, and blood. Adult "canyon flies" (Fannia conspicua Malloch) were collected from the coastal mountain community of La Habra Heights in Los Angeles County, CA, during late spring and early summer of 2011. Canyon flies were colonized in the laboratory and maintained for over two years. Larval growth, sex-specific adult emergence, and fecundity were evaluated quantitatively. At 25°C, male flies required a minimum of 22 d postoviposition to develop from egg to adult, with peak emergence occurring at 24-26 d; female flies required a minimum of 25 d postoviposition, with peak emergence occurring at 26-28 d. Female flies were capable of oviposition starting at 7 d postemergence, and produced a mean of 90.6±54.7 eggs over a 19-41-d life span. Canyon fly eggs were quite resistant to desiccation, with hatching rate of eggs reduced only after ≥12 wk of desiccation, and some successful hatching even when eggs were desiccated up to 28 wk. When immature flies were removed from their food source and subjected to continuous desiccation and starvation, flies at 3-d-old posthatching did not survive, and only 8% of flies at 5-d posthatching survived and completed development to the adult stage. Immature flies that were 7- or 11-d-old posthatching survived to adulthood in good numbers, having reached the mid to late L3 stage at the time of food removal. Exposure to desiccation in either the egg or larval stage had no effect on fecundity of surviving females. This extreme level of desiccation tolerance is likely an adaptation to increase survival in desert climates of the southwestern United States with long, hot summers and little precipitation, where Fannia conspicua are typically distributed. PMID:26335459

  12. Laboratory Colonization, Life History Observations, and Desiccation Tolerance of the Canyon Fly Fannia conspicua (Diptera: Fanniidae).

    PubMed

    Zhang, Congcong; Gerry, Alec C

    2015-07-01

    "Canyon flies" are flies within the Fannia benjamini Malloch species complex. These flies can be considerable pests to humans and animals due to their habit of feeding on animal body secretions such as tears, mucus, sweat, and blood. Adult "canyon flies" (Fannia conspicua Malloch) were collected from the coastal mountain community of La Habra Heights in Los Angeles County, CA, during late spring and early summer of 2011. Canyon flies were colonized in the laboratory and maintained for over two years. Larval growth, sex-specific adult emergence, and fecundity were evaluated quantitatively. At 25°C, male flies required a minimum of 22 d postoviposition to develop from egg to adult, with peak emergence occurring at 24-26 d; female flies required a minimum of 25 d postoviposition, with peak emergence occurring at 26-28 d. Female flies were capable of oviposition starting at 7 d postemergence, and produced a mean of 90.6±54.7 eggs over a 19-41-d life span. Canyon fly eggs were quite resistant to desiccation, with hatching rate of eggs reduced only after ≥12 wk of desiccation, and some successful hatching even when eggs were desiccated up to 28 wk. When immature flies were removed from their food source and subjected to continuous desiccation and starvation, flies at 3-d-old posthatching did not survive, and only 8% of flies at 5-d posthatching survived and completed development to the adult stage. Immature flies that were 7- or 11-d-old posthatching survived to adulthood in good numbers, having reached the mid to late L3 stage at the time of food removal. Exposure to desiccation in either the egg or larval stage had no effect on fecundity of surviving females. This extreme level of desiccation tolerance is likely an adaptation to increase survival in desert climates of the southwestern United States with long, hot summers and little precipitation, where Fannia conspicua are typically distributed.

  13. Liquid-Cooled Garment

    NASA Technical Reports Server (NTRS)

    1977-01-01

    A liquid-cooled bra, offshoot of Apollo moon suit technology, aids the cancer-detection technique known as infrared thermography. Water flowing through tubes in the bra cools the skin surface to improve resolution of thermograph image.

  14. Liquid cooled garments

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Liquid cooled garments employed in several applications in which severe heat is encountered are discussed. In particular, the use of the garments to replace air line cooling units in a variety of industrial processing situations is discussed.

  15. Radial turbine cooling

    NASA Astrophysics Data System (ADS)

    Roelke, Richard J.

    The technology of high temperature cooled radial turbines is reviewed. Aerodynamic performance considerations are described. Heat transfer and structural analysis are addressed, and in doing so the following topics are covered: cooling considerations, hot side convection, coolant side convection, and rotor mechanical analysis. Cooled rotor concepts and fabrication are described, and the following are covered in this context: internally cooled rotor, hot isostatic pressure bonded rotor, laminated rotor, split blade rotor, and the NASA radial turbine program.

  16. Radial turbine cooling

    NASA Technical Reports Server (NTRS)

    Roelke, Richard J.

    1992-01-01

    The technology of high temperature cooled radial turbines is reviewed. Aerodynamic performance considerations are described. Heat transfer and structural analysis are addressed, and in doing so the following topics are covered: cooling considerations, hot side convection, coolant side convection, and rotor mechanical analysis. Cooled rotor concepts and fabrication are described, and the following are covered in this context: internally cooled rotor, hot isostatic pressure bonded rotor, laminated rotor, split blade rotor, and the NASA radial turbine program.

  17. Data center cooling system

    DOEpatents

    Chainer, Timothy J; Dang, Hien P; Parida, Pritish R; Schultz, Mark D; Sharma, Arun

    2015-03-17

    A data center cooling system may include heat transfer equipment to cool a liquid coolant without vapor compression refrigeration, and the liquid coolant is used on a liquid cooled information technology equipment rack housed in the data center. The system may also include a controller-apparatus to regulate the liquid coolant flow to the liquid cooled information technology equipment rack through a range of liquid coolant flow values based upon information technology equipment temperature thresholds.

  18. Comparative study of different solar cooling systems for buildings in subtropical city

    SciTech Connect

    Fong, K.F.; Chow, T.T.; Lee, C.K.; Lin, Z.; Chan, L.S.

    2010-02-15

    In recent years, more and more attention has been paid on the application potential of solar cooling for buildings. Due to the fact that the efficiency of solar collectors is generally low at the time being, the effectiveness of solar cooling would be closely related to the availability of solar irradiation, climatic conditions and geographical location of a place. In this paper, five types of solar cooling systems were involved in a comparative study for subtropical city, which is commonly featured with long hot and humid summer. The solar cooling systems included the solar electric compression refrigeration, solar mechanical compression refrigeration, solar absorption refrigeration, solar adsorption refrigeration and solar solid desiccant cooling. Component-based simulation models of these systems were developed, and their performances were evaluated throughout a year. The key performance indicators are solar fraction, coefficient of performance, solar thermal gain, and primary energy consumption. In addition, different installation strategies and types of solar collectors were compared for each kind of solar cooling system. Through this comparative study, it was found that solar electric compression refrigeration and solar absorption refrigeration had the highest energy saving potential in the subtropical Hong Kong. The former is to make use of the solar electric gain, while the latter is to adopt the solar thermal gain. These two solar cooling systems would have even better performances through the continual advancement of the solar collectors. It will provide a promising application potential of solar cooling for buildings in the subtropical region. (author)

  19. Impact of body melanisation on desiccation resistance in montane populations of D. melanogaster: Analysis of seasonal variation.

    PubMed

    Parkash, Ravi; Sharma, Vineeta; Kalra, Bhawna

    2009-10-01

    In the montane localities of subtropical regions, winter is the dry season and ectothermic drosophilids are expected to evolve desiccation resistance to cope with drier climatic conditions. An analysis of six montane populations (600-2226m) of D. melanogaster showed variations for body melanisation (i.e. pigmentation) and desiccation resistance across seasons as well as along altitude. During winter season, plastic changes for melanisation of three posterior abdominal segments (5th+6th+7th) correspond with higher desiccation resistance. Thus, we analyzed genetic and plastic effects for these ecophysiological traits by comparing wild-caught and laboratory reared individuals of D. melanogaster for autumn as well as winter season. A ratio of slope values in wild vs. laboratory populations has shown a 1.64-fold plastic effect during autumn; and a two-fold effect during winter. For body melanisation and desiccation resistance, evolutionary response to altitudinal environmental gradient is similar to the phenotypic response across seasons. Thus, our observations are in agreement with the co-gradient hypothesis. Further, we tested the hypothesis whether a thicker cuticle (either due to melanisation or cuticular lipids) leads to lesser cuticular water loss and higher desiccation resistance across seasons as well as according to altitude. Based on within and between population analyses, body melanisation was found to be positively correlated with desiccation resistance but negatively with cuticular water loss. Interestingly, there were no changes in the amount of cuticular lipids per fly across seasons as well as along altitude; and therefore cuticular lipids did not account for desiccation resistance. Cuticular water loss exhibited negative correlation with body melanisation but not with cuticular lipids as well as with changes in body size across seasons. Thus, our data suggest that seasonal changes in body melanisation confer desiccation resistance in montane populations

  20. Cool flame quench distances

    NASA Technical Reports Server (NTRS)

    Ryason, P. R.; Hirsch, E.

    1974-01-01

    The results of a brief experimental investigation are presented which confirm the expectation that cool flame quenching distances should be larger than hot flame quenching distances. It is also discovered that whereas quenching distances for hot flames reach their minimum values near stoichiometric conditions, cool flame quenching distances are least under rich conditions. Rich conditions are well known to favor cool flame formation.

  1. Cooling of stored beams

    SciTech Connect

    Mills, F.E.

    1986-10-15

    Beam cooling methods developed for the accumulation of antiprotons are being employed to assist in the performance of experiments in Nuclear and Particle Physics with ion beams stored in storage rings. The physics of beam cooling, and the ranges of utility of stochastic and electron cooling are discussed in this paper.

  2. Stochastic cooling in RHIC

    SciTech Connect

    Brennan,J.M.; Blaskiewicz, M. M.; Severino, F.

    2009-05-04

    After the success of longitudinal stochastic cooling of bunched heavy ion beam in RHIC, transverse stochastic cooling in the vertical plane of Yellow ring was installed and is being commissioned with proton beam. This report presents the status of the effort and gives an estimate, based on simulation, of the RHIC luminosity with stochastic cooling in all planes.

  3. Plant Drought Tolerance Enhancement by Trehalose Production of Desiccation-Tolerant Microorganisms

    PubMed Central

    Vílchez, Juan I.; García-Fontana, Cristina; Román-Naranjo, Desireé; González-López, Jesús; Manzanera, Maximino

    2016-01-01

    A collection of desiccation-tolerant xeroprotectant-producing microorganisms was screened for their ability to protect plants against drought, and their role as plant growth-promoting rhizobacteria was investigated in two different crops (tomato and pepper). The most commonly described biochemical mechanisms for plant protection against drought by microorganisms including the production of phytohormones, antioxidants and xeroprotectants were analyzed. In particular, the degree of plant protection against drought provided by these microorganisms was characterized. After studying the findings and comparing them with results of the closest taxonomic relatives at the species and strain levels, we propose that trehalose produced by these microorganisms is correlated with their ability to protect plants against drought. This proposal is based on the increased protection of plants against drought by the desiccation-sensitive microorganism Pseudomonas putida KT2440, which expresses the otsAB genes for trehalose biosynthesis in trans. PMID:27746776

  4. Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells

    PubMed Central

    Bissoyi, Akalabya; Gusev, Oleg; Patra, Pradeep Kumar

    2016-01-01

    Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context. Loading of the protectant in the live cell can be a challenging issue but recent studies have shown encouraging results. This paper deals with a review of the protectants, methods of their delivery, and physical boundary conditions adopted for the desiccation and lyophilisation of mammalian cells, including MSCs. A hybrid technique combining both methods is also proposed as a promising way of MSCs dry preservation. PMID:27597869

  5. Contrasting assembly processes in a bacterial metacommunity along a desiccation gradient

    PubMed Central

    Valverde, Angel; Makhalanyane, Thulani P.; Cowan, Don A.

    2014-01-01

    Understanding the relative influence of deterministic and stochastic processes in driving community assembly is a major goal in microbial ecology. Here, we have investigated the influence of these processes on bacterial community assembly in the lateral sediments of a salt pan along a desiccation gradient over a three-year period. We show that the role of deterministic processes increases in communities distant from the water line (shaped by drought), probably as a result of the interplay between abiotic and biotic factors. By contrast, the influence of stochastic processes on bacterial community assembly was higher in the sediments closest to the water line, more likely due to lower levels of abiotic stress. Our results demonstrate that both deterministic and stochastic processes influence bacterial community assembly in salt pan sediments, and that their relative influence varies along a desiccation gradient. PMID:25520714

  6. Dehumidification Performance of Hybrid Type Humidity Control System Coupling a Desiccant Rotor in a Refrigeration Cycle

    NASA Astrophysics Data System (ADS)

    Horibe, Akihiko; Takaki, Sadao; Inaba, Hideo; Haruki, Naoto

    Desiccant air-conditioning system is a promising technology because the exhaust heat can be effectively used in the future. We have reported the proposed system that combines a desiccant rotor with a vapor compression refrigerator. The confirmation experiment of stability and the performance was conducted with the experimental prototype. The result showed that it had the performance that was necessary for dehumidification driving in the summer and the humidification driving in the winter. In this report, we examined the influence on humidity controlling performance of the processing air temperature and humidity. As a result, we got high dehumidification efficiency and clarified the dehumidification characteristic in dehumidification driving in the summer. Dehumidification efficiency about 4.0 kg/kWh and COP of the system about 2.0 in summer driving mode were obtained.

  7. Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells

    PubMed Central

    Bissoyi, Akalabya; Gusev, Oleg; Patra, Pradeep Kumar

    2016-01-01

    Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context. Loading of the protectant in the live cell can be a challenging issue but recent studies have shown encouraging results. This paper deals with a review of the protectants, methods of their delivery, and physical boundary conditions adopted for the desiccation and lyophilisation of mammalian cells, including MSCs. A hybrid technique combining both methods is also proposed as a promising way of MSCs dry preservation.

  8. Seed storage proteins of spermatophytes share a common ancestor with desiccation proteins of fungi.

    PubMed

    Bäumlein, H; Braun, H; Kakhovskaya, I A; Shutov, A D

    1995-12-01

    The legumin- and vicilin-like seed storage globulins of spermatophytes are specifically accumulated during embryogenesis and seed development. Previous studies have shown that a precursor common to both legumin and vicilin genes might have evolved by duplication from a single-domain ancestral gene. We here report that amino acid sequences of legumin and vicilin domains share statistically significant similarity to the germination-specific germins of wheat as well as to the spherulation-specific spherulins of myxomycetes. This conclusion is further supported by the derived intron-exon structure of a spherulin gene. Spherulins are thought to be involved in tissue desiccation or hydration. It is suggested that the present-day seed globulins of spermatophytes have evolved from a group of ancient proteins functional in cellular desiccation/hydration processes.

  9. Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells.

    PubMed

    Bissoyi, Akalabya; Kumar, Awanish; Rizvanov, Albert A; Nesmelov, Alexander; Gusev, Oleg; Patra, Pradeep Kumar; Bit, Arindam

    2016-01-01

    Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context. Loading of the protectant in the live cell can be a challenging issue but recent studies have shown encouraging results. This paper deals with a review of the protectants, methods of their delivery, and physical boundary conditions adopted for the desiccation and lyophilisation of mammalian cells, including MSCs. A hybrid technique combining both methods is also proposed as a promising way of MSCs dry preservation.

  10. Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells.

    PubMed

    Bissoyi, Akalabya; Kumar, Awanish; Rizvanov, Albert A; Nesmelov, Alexander; Gusev, Oleg; Patra, Pradeep Kumar; Bit, Arindam

    2016-01-01

    Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context. Loading of the protectant in the live cell can be a challenging issue but recent studies have shown encouraging results. This paper deals with a review of the protectants, methods of their delivery, and physical boundary conditions adopted for the desiccation and lyophilisation of mammalian cells, including MSCs. A hybrid technique combining both methods is also proposed as a promising way of MSCs dry preservation. PMID:27597869

  11. Differential effects of abscisic acid on desiccation tolerance and carbohydrates in three species of liverworts.

    PubMed

    Pence, Valerie C; Dunford, Susan S; Redella, Steven

    2005-12-01

    Tissues of three species of in vitro grown liverworts, Riccia fluitans, Pallavicinia lyellii, and Marchantia polymorpha, were subjected to rapid drying with and without preculture for 1 week on medium containing 10 microM ABA. ABA preculture initiated total desiccation tolerance in R. fluitans, whereas control tissues were killed after 30 min of drying. Survival was also improved in P. lyellii, whereas ABA did not affect survival of M. polymorpha after rapid drying. ABA treatment did, however, reduce the rate of water loss in M. polymorpha. Total soluble carbohydrates were increased in ABA-treated R. fluitans and P. lyellii, but not in M. polymorpha, although there was no correlation between survival and changes in the percentage of these carbohydrates as reducing sugars. These differences in response to ABA and desiccation likely reflect different adaptations of these three species to conditions in situ.

  12. Towards clarifying what distinguishes cyanobacteria able to resurrect after desiccation from those that cannot: The photosynthetic aspect.

    PubMed

    Raanan, Hagai; Oren, Nadav; Treves, Haim; Keren, Nir; Ohad, Itzhak; Berkowicz, Simon M; Hagemann, Martin; Koch, Moriz; Shotland, Yoram; Kaplan, Aaron

    2016-06-01

    Organisms inhabiting biological soil crusts (BSCs) are able to cope with extreme environmental conditions including daily hydration/dehydration cycles, high irradiance and extreme temperatures. The photosynthetic machinery, potentially the main source of damaging reactive oxygen species during cessation of CO(2) fixation in desiccating cells, must be protected to avoid sustained photodamage. We compared certain photosynthetic parameters and the response to excess light of BCS-inhabiting, desiccation-tolerant cyanobacteria Leptolyngbya ohadii and Nostoc reinholdii with those observed in the "model" organisms Nostoc sp. PCC 7120, able to resurrect after mild desiccation, and Synechococcus elongatus PCC 7942 and Synechocystis sp. PCC 6803 that are unable to recover from dehydration. Desiccation-tolerant strains exhibited a transient decline in the photosynthetic rate at light intensities corresponding to the inflection point in the PI curve relating the O(2) evolution rate to light intensity. They also exhibited a faster and larger loss of variable fluorescence and profoundly faster Q(A)(-) re-oxidation rates after exposure to high illumination. Finally, a smaller difference was found in the temperature of maximal thermoluminescence signal in the absence or presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) than observed in "model" cyanobacteria. These parameters indicate specific functional differences of photosystem II (PSII) between desiccation tolerant and sensitive cyanobacteria. We propose that exposure to excess irradiation activates a non-radiative electron recombination route inside PSII that minimizes formation of damaging singlet oxygen in the desiccation-tolerant cyanobacteria and thereby reduces photodamage. PMID:26896589

  13. Dehydration-Induced Redistribution of Amphiphilic Molecules between Cytoplasm and Lipids Is Associated with Desiccation Tolerance in Seeds1

    PubMed Central

    Buitink, Julia; Leprince, Olivier; Hoekstra, Folkert A.

    2000-01-01

    This study establishes a relationship between desiccation tolerance and the transfer of amphiphilic molecules from the cytoplasm into lipids during drying, using electron paramagnetic resonance spectroscopy of amphiphilic spin probes introduced into imbibed radicles of pea (Pisum sativum) and cucumber (Cucumis sativa) seeds. Survival following drying and a membrane integrity assay indicated that desiccation tolerance was present during early imbibition and lost in germinated radicles. In germinated cucumber radicles, desiccation tolerance could be re-induced by an incubation in polyethylene glycol (PEG) before drying. In desiccation-intolerant radicles, partitioning of spin probes into lipids during dehydration occurred at higher water contents compared with tolerant and PEG-induced tolerant radicles. The difference in partitioning behavior between desiccation-tolerant and -intolerant tissues could not be explained by the loss of water. Consequently, using a two-phase model system composed of sunflower or cucumber oil and water, physical properties of the aqueous solvent that may affect the partitioning of amphiphilic spin probes were investigated. A significant relationship was found between the partitioning of spin probes and the viscosity of the aqueous solvent. Moreover, in desiccation-sensitive radicles, the rise in cellular microviscosity during drying commenced at higher water contents compared with tolerant or PEG-induced tolerant radicles, suggesting that the microviscosity of the cytoplasm may control the partitioning behavior in dehydrating seeds. PMID:11080316

  14. Towards clarifying what distinguishes cyanobacteria able to resurrect after desiccation from those that cannot: The photosynthetic aspect.

    PubMed

    Raanan, Hagai; Oren, Nadav; Treves, Haim; Keren, Nir; Ohad, Itzhak; Berkowicz, Simon M; Hagemann, Martin; Koch, Moriz; Shotland, Yoram; Kaplan, Aaron

    2016-06-01

    Organisms inhabiting biological soil crusts (BSCs) are able to cope with extreme environmental conditions including daily hydration/dehydration cycles, high irradiance and extreme temperatures. The photosynthetic machinery, potentially the main source of damaging reactive oxygen species during cessation of CO(2) fixation in desiccating cells, must be protected to avoid sustained photodamage. We compared certain photosynthetic parameters and the response to excess light of BCS-inhabiting, desiccation-tolerant cyanobacteria Leptolyngbya ohadii and Nostoc reinholdii with those observed in the "model" organisms Nostoc sp. PCC 7120, able to resurrect after mild desiccation, and Synechococcus elongatus PCC 7942 and Synechocystis sp. PCC 6803 that are unable to recover from dehydration. Desiccation-tolerant strains exhibited a transient decline in the photosynthetic rate at light intensities corresponding to the inflection point in the PI curve relating the O(2) evolution rate to light intensity. They also exhibited a faster and larger loss of variable fluorescence and profoundly faster Q(A)(-) re-oxidation rates after exposure to high illumination. Finally, a smaller difference was found in the temperature of maximal thermoluminescence signal in the absence or presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) than observed in "model" cyanobacteria. These parameters indicate specific functional differences of photosystem II (PSII) between desiccation tolerant and sensitive cyanobacteria. We propose that exposure to excess irradiation activates a non-radiative electron recombination route inside PSII that minimizes formation of damaging singlet oxygen in the desiccation-tolerant cyanobacteria and thereby reduces photodamage.

  15. Phenomic and transcriptomic analyses reveal that autophagy plays a major role in desiccation tolerance in Saccharomyces cerevisiae.

    PubMed

    Ratnakumar, Sooraj; Hesketh, Andy; Gkargkas, Konstantinos; Wilson, Michael; Rash, Bharat M; Hayes, Andrew; Tunnacliffe, Alan; Oliver, Stephen G

    2011-01-01

    Saccharomyces cerevisiae can survive extreme desiccation, but the molecular mechanisms are poorly understood. To define genes involved in desiccation tolerance, two complementary genome-wide approaches, phenomics and transcriptomics, have been used, together with a targeted analysis of gene deletion mutants tested individually for their ability to survive drying. Genome-wide phenotypic analyses carried out on a pooled library of single-gene deletion mutants subjected to three cycles of desiccation and re-growth to post-diauxic phase identified about 650 genes that contributed to strain survival in the drying process. Air-drying desiccation-tolerant post-diauxic phase cells significantly altered transcription in 12% of the yeast genome, activating expression of over 450 genes and down-regulating 330. Autophagy processes were significantly over-represented in both the phenomics study and the genes up-regulated on drying, indicating the importance of the clearance of protein aggregates/damaged organelles and the recycling of nutrients for the survival of desiccation in yeast. Functional carbon source sensing networks governed by the PKA, Tor and Snf1 protein kinase complexes were important for the survival of desiccation, as indicated by phenomics, transcriptomics, and individual analyses of mutant strains. Changes in nitrogen metabolism were evident during the drying process and parts of the environmental stress response were activated, repressing ribosome production and inducing genes for coping with oxidative and osmotic stress. PMID:20963216

  16. Dehydration-induced redistribution of amphiphilic molecules between cytoplasm and lipids is associated with desiccation tolerance in seeds.

    PubMed

    Buitink, J; Leprince, O; Hoekstra, F A

    2000-11-01

    This study establishes a relationship between desiccation tolerance and the transfer of amphiphilic molecules from the cytoplasm into lipids during drying, using electron paramagnetic resonance spectroscopy of amphiphilic spin probes introduced into imbibed radicles of pea (Pisum sativum) and cucumber (Cucumis sativa) seeds. Survival following drying and a membrane integrity assay indicated that desiccation tolerance was present during early imbibition and lost in germinated radicles. In germinated cucumber radicles, desiccation tolerance could be re-induced by an incubation in polyethylene glycol (PEG) before drying. In desiccation-intolerant radicles, partitioning of spin probes into lipids during dehydration occurred at higher water contents compared with tolerant and PEG-induced tolerant radicles. The difference in partitioning behavior between desiccation-tolerant and -intolerant tissues could not be explained by the loss of water. Consequently, using a two-phase model system composed of sunflower or cucumber oil and water, physical properties of the aqueous solvent that may affect the partitioning of amphiphilic spin probes were investigated. A significant relationship was found between the partitioning of spin probes and the viscosity of the aqueous solvent. Moreover, in desiccation-sensitive radicles, the rise in cellular microviscosity during drying commenced at higher water contents compared with tolerant or PEG-induced tolerant radicles, suggesting that the microviscosity of the cytoplasm may control the partitioning behavior in dehydrating seeds.

  17. Localization and Quantification of Callose in the Streptophyte Green Algae Zygnema and Klebsormidium: Correlation with Desiccation Tolerance

    PubMed Central

    Herburger, Klaus; Holzinger, Andreas

    2015-01-01

    Freshwater green algae started to colonize terrestrial habitats about 460 million years ago, giving rise to the evolution of land plants. Today, several streptophyte green algae occur in aero-terrestrial habitats with unpredictable fluctuations in water availability, serving as ideal models for investigating desiccation tolerance. We tested the hypothesis that callose, a β-d-1,3-glucan, is incorporated specifically in strained areas of the cell wall due to cellular water loss, implicating a contribution to desiccation tolerance. In the early diverging genus Klebsormidium, callose was drastically increased already after 30 min of desiccation stress. Localization studies demonstrated an increase in callose in the undulating cross cell walls during cellular water loss, allowing a regulated shrinkage and expansion after rehydration. This correlates with a high desiccation tolerance demonstrated by a full recovery of the photosynthetic yield visualized at the subcellular level by Imaging-PAM. Furthermore, abundant callose in terminal cell walls might facilitate cell detachment to release dispersal units. In contrast, in the late diverging Zygnema, the callose content did not change upon desiccation for up to 3.5 h and was primarily localized in the corners between individual cells and at terminal cells. While these callose deposits still imply reduction of mechanical damage, the photosynthetic yield did not recover fully in the investigated young cultures of Zygnema upon rehydration. The abundance and specific localization of callose correlates with the higher desiccation tolerance in Klebsormidium when compared with Zygnema. PMID:26412780

  18. Prediction of Desiccation Sensitivity in Seeds of Woody Species: A Probabilistic Model Based on Two Seed Traits and 104 Species

    PubMed Central

    DAWS, M. I.; GARWOOD, N. C.; PRITCHARD, H. W.

    2006-01-01

    • Background and Aims Seed desiccation sensitivity limits the ex situ conservation of up to 47 % of plant species, dependent on habitat. Whilst desirable, empirically determining desiccation tolerance levels in seeds of all species is unrealistic. A probabilistic model for the rapid identification of woody species at high risk of displaying seed desiccation sensitivity is presented. • Methods The model was developed using binary logistic regression on seed trait data [seed mass, moisture content, seed coat ratio (SCR) and rainfall in the month of seed dispersal] for 104 species from 37 families from a semi-deciduous tropical forest in Panamá. • Key Results For the Panamanian species, only seed mass and SCR were significantly related to the response to desiccation, with the desiccation-sensitive seeds being large and having a relatively low SCR (i.e. thin ‘seed’ coats). Application of this model to a further 38 species, of known seed storage behaviour, from two additional continents and differing vegetation types (dryland Africa and temperate Europe) correctly predicted the response to desiccation in all cases, and resolved conflicting published data for two species (Acer pseudoplatanus and Azadirachta indica). • Conclusions This model may have application as a decision-making tool in the handling of species of unknown seed storage behaviour in species from three disparate habitats. PMID:16464874

  19. Microbial biosynthesis of wax esters during desiccation: an adaptation for colonization of the earliest terrestrial environments?

    NASA Astrophysics Data System (ADS)

    Finkelstein, D. B.; Brassell, S. C.; Pratt, L. M.

    2008-12-01

    Biosynthesis of wax esters (WE) by prokaryotes in natural systems, notably bacteria from hot springs and marine phytoplankton, is poorly documented, primarily because saponification is a routine step in the analysis of microbial mat lipids. Use of this preparative procedure, critical for characterization of the diagnostic distributions of carboxylic acids in phospholipids, precludes recovery of intact WE. Examination of non-saponified lipids in emergent and desiccated mats with comparable microbial communities from the Warner Lake region, Oregon, reveals increases in the relative abundance (18.6 to 59.9μg/g Corg) and average chain length (C38 to C46) of WE in the latter, combined with assimilation of phytol and tocopherol moieties. Prokaryotes can accumulate WE as storage lipids in vitro, notably at elevated temperature or under nitrogen limiting conditions, but we propose that biosynthesis of long-chain WE that have a low solubility and are resistant to degradation/oxidation may represent an evolutionary strategy to survive desiccation in evaporative environments. Moreover, aeolian transport of desiccated mat-rip-ups between lake flats allows for migration of microbial communities within and between lake flats and basins during arid conditions. Subsequent rehydration within an alkaline environment would naturally saponify WE, and thereby regenerate alcohol and acid moieties that could serve as membrane lipids for the next viable microbial generation. The evolutionary cradle of WE was likely abiotic generation under hydrothermal conditions, which is consistent with the antiquity of the ester linkage necessitated by its integral role in the membranes of Eubacteria (though not Archaea) and in bacteriochlorophyll. The subsequent capability of microbes to biosynthesize WE may have facilitated their survival when nutrients were limiting, and production of long-chain WE (>C40) may represent a further critical evolutionary threshold that enabled their persistence through

  20. Coherent optical analysis of crystal-like patterns induced by human blood plasma desiccation

    NASA Astrophysics Data System (ADS)

    Kulyabina, Tatyana V.; Drajevsky, Roman A.; Kochubey, Vyacheslav I.; Zimnyakov, Dmitry A.

    2001-05-01

    The comparative investigation of blood plasma and serum polycrystal layers properties was executed. Formation of microcrystals by desiccation of blood plasma is shown. Such crystals are absent in serum layers. The formation of thrombus at blood serum preparation and, as a result, lack of protein molecules is the reason of the difference. The possibility of Wiener spectra application for analysis of formed crystal structure disorder is shown.

  1. Desiccation tolerance, longevity and seed-siring ability of entomophilous pollen from UK native orchid species

    PubMed Central

    Marks, Timothy R.; Seaton, Philip T.; Pritchard, Hugh W.

    2014-01-01

    Background and Aims Pollinator-limited seed-set in some terrestrial orchids is compensated for by the presence of long-lived flowers. This study tests the hypothesis that pollen from these insect-pollinated orchids should be desiccation tolerant and relatively long lived using four closely related UK terrestrial species; Anacamptis morio, Dactylorhiza fuchsii, D. maculata and Orchis mascula. Methods Pollen from the four species was harvested from inflorescences and germinated in vitro, both immediately and also after drying to simulate interflower transit. Their tolerance to desiccation and short-term survival was additionally assessed after 3 d equilibration at a range of relative humidities (RHs), and related to constructed sorption isotherms (RH vs. moisture content, MC). Ageing of D. fuchsii pollen was further tested over 2 months against temperature and RH, and the resultant survival curves were subjected to probit analysis, and the distribution of pollen death in time (σ) was determined. The viability and siring ability, following artificial pollinations, were determined in D. fuchsii pollen following storage for 6 years at –20 °C. Key Results The pollen from all four species exhibited systematic increases in germinability and desiccation tolerance as anthesis approached, and pollen from open flowers generally retained high germinability. Short-term storage revealed sensitivity to low RH, whilst optimum survival occurred at comparable RHs in all species. Similarly, estimated pollen life spans (σ) at differing temperatures were longest under the dry conditions. Despite a reduction in germination and seeds per capsule, long-term storage of D. fuchsii pollen did not impact on subsequent seed germination in vitro. Conclusions Substantial pollen desiccation tolerance and life span of the four entomophilous orchids reflects a resilient survival strategy in response to unpredictable pollinator visitation, and presents an alternative approach to germplasm

  2. Desiccation cracks in vertic palaeosols of the Cretaceous Hasandong Formation, Korea: genesis and palaeoenvironmental implications

    NASA Astrophysics Data System (ADS)

    Paik, In Sung; Lee, Yong Il

    1998-07-01

    The Hasandong Formation (Upper Cretaceous), Korea contains diagnostic vertic features such as pedogenic slickensides, pseudoanticlines, and large desiccation cracks. The Hasandong vertic palaeosols developed on floodplain deposits and display a diverse range of desiccation cracks, including calcite-filled, vertically stratified, and thin varieties. The calcite-filled cracks are 5-10 cm wide on the surface and up to 60 cm deep, and may have been formed by selective calcite precipitation in the cracks due to preferential vegetation. The vertically stratified cracks are those with crack-fillings having alternating sandstone and mudstone subparallel to crack walls. They are interpreted to have been formed by repeated crack opening and filling at crack margins due to seasonal drying and wetting. The thin cracks are less than 1 mm to a few mm wide and a few cm long, and are recognized by light colour mottling around them. Thin crack development may have resulted from intermittent incomplete cracking during sedimentation. The Hasandong vertic palaeosols have stratigraphic and lateral variations in vertic features, which are attributed to the changes in palaeoenvironments. Deep and large desiccation cracks with unbridged sediment-infilling formed in flood basins. The low topographic position of the distal floodbasin area resulted in weakly developed vertic palaeosols with calcite-filled desiccation cracks and some rhizocretions, and proximal location to a main channel may be responsible for rare development of deep and wide cracks and intercalation with calcrete-intraclast conglomerates. Differences in these vertic features along with interbedded calcic palaeosols may provide useful criteria for the palaeoenvironmental changes in and correlation of the floodplain deposits.

  3. Functional genomic and phenotypic responses to desiccation in natural populations of a desert drosophilid

    PubMed Central

    Rajpurohit, Subhash; Oliveira, Cássia C.; Etges, William J.; Gibbs, Allen G.

    2014-01-01

    We used whole transcriptome microarrays to assess changes in gene expression and monitored mortality rates and epicuticular hydrocarbons (CHCs) in response to desiccation stress in four natural populations of Drosophila mojavensis from Baja California and mainland Mexico. Desiccation had the greatest effect on gene expression, followed by biogeographical variation at regional and population levels. Genes involved in environmental sensing and cuticular structure were up-regulated in dry conditions, while genes involved in transcription itself were down-regulated. Flies from Baja California had higher expression of reproductive and mitochondrial genes, suggesting that these populations have greater fecundity and higher metabolic rates. Host plant differences had a surprisingly minor effect on the transcriptome. In most cases, desiccation-caused mortality was greater in flies reared on fermenting cactus tissues than laboratory media. Water content of adult females and males was significantly different, and was lower in Baja California males. Different groups of CHCs simultaneously increased and decreased in amounts due to desiccation exposure of 9 and 18 hr and were population-specific and dependent on larval rearing substrates. Overall, we observed that changes in gene expression involved a coordinated response of behavioral, cuticular and metabolic genes. Together with differential expression of cuticular hydrocarbons, this study revealed some of the mechanisms that have allowed D. mojavensis to exploit its harsh desert conditions. Certainly, for D. mojavensis that uses different host plants, population-level understanding of responses to stressors associated with future climate change in desert regions must be evaluated across geographical and local ecological scales. PMID:23505972

  4. Cooling water distribution system

    DOEpatents

    Orr, Richard

    1994-01-01

    A passive containment cooling system for a nuclear reactor containment vessel. Disclosed is a cooling water distribution system for introducing cooling water by gravity uniformly over the outer surface of a steel containment vessel using an interconnected series of radial guide elements, a plurality of circumferential collector elements and collector boxes to collect and feed the cooling water into distribution channels extending along the curved surface of the steel containment vessel. The cooling water is uniformly distributed over the curved surface by a plurality of weirs in the distribution channels.

  5. Heating and cooling system

    SciTech Connect

    Imig, L.A.; Gardner, M.R.

    1982-08-01

    A heating and cooling apparatus capable of cyclic heating and cooling of a test specimen undergoing fatigue testing is discussed. Cryogenic fluid is passed through a block clamped to the speciment to cool the block and the specimen. Heating cartridges penetrate the block to heat the block and the specimen to very hot temperaures. Control apparatus is provided to alternatively activate the cooling and heating modes to effect cyclic heating and cooling between very hot and very cold temperatures. The block is constructed of minimal mass to facilitate the rapid temperature changes. Official Gazette of the U.S. Patent and Trademark Office.

  6. Postexercise Cooling Rates in 2 Cooling Jackets

    PubMed Central

    Brade, Carly; Dawson, Brian; Wallman, Karen; Polglaze, Ted

    2010-01-01

    Abstract Context: Cooling jackets are a common method for removing stored heat accumulated during exercise. To date, the efficiency and practicality of different types of cooling jackets have received minimal investigation. Objective: To examine whether a cooling jacket containing a phase-change material (PC17) results in more rapid postexercise cooling than a gel cooling jacket and a no-jacket (control) condition. Design: Randomized, counterbalanced design with 3 experimental conditions. Setting: Participants exercised at 75% V̇o2max workload in a hot climate chamber (temperature  =  35.0 ± 1.4°C, relative humidity  =  52 ± 4%) for 30 minutes, followed by postexercise cooling for 30 minutes in cool laboratory conditions (ambient temperature  =  24.9 ± 1.8°C, relative humidity  =  39% ± 10%). Patients or Other Participants: Twelve physically active men (age  =  21.3 ± 1.1 years, height  =  182.7 ± 7.1 cm, body mass  =  76.2 ± 9.5 kg, sum of 6 skinfolds  =  50.5 ± 6.9 mm, body surface area  =  1.98 ± 0.14 m2, V̇o2max  =  49.0 ± 7.0 mL·kg−1·min−1) participated. Intervention(s): Three experimental conditions, consisting of a PC17 jacket, a gel jacket, and no jacket. Main Outcome Measure(s): Core temperature (TC), mean skin temperature (TSk), and TC cooling rate (°C/min). Results: Mean peak TC postexercise was 38.49 ± 0.42°C, 38.57 ± 0.41°C, and 38.55 ± 0.40°C for the PC17 jacket, gel jacket, and control conditions, respectively. No differences were observed in peak TC cooling rates among the PC17 jacket (0.038 ± 0.007°C/min), gel jacket (0.040 ± 0.009°C/min), and control (0.034 ± 0.010°C/min, P > .05) conditions. Between trials, no differences were calculated for mean TSk cooling. Conclusions: Similar cooling rates for all 3 conditions indicate that there is no benefit associated with wearing the PC17 or gel jacket. PMID:20210620

  7. Late embryogenesis abundant proteins protect human hepatoma cells during acute desiccation

    PubMed Central

    Li, Shumin; Chakraborty, Nilay; Borcar, Apurva; Menze, Michael A.; Toner, Mehmet; Hand, Steven C.

    2012-01-01

    Expression of late embryogenesis abundant (LEA) proteins is highly correlated with desiccation tolerance in anhydrobiotic animals, selected land plants, and bacteria. Genes encoding two LEA proteins, one localized to the cytoplasm/nucleus (AfrLEA2) and one targeted to mitochondria (AfrLEA3m), were stably transfected into human HepG2 cells. A trehalose transporter was used for intracellular loading of this disaccharide. Cells were rapidly and uniformly desiccated to low water content (<0.12 g H2O/g dry weight) with a recently developed spin-drying technique. Immediately on rehydration, control cells without LEA proteins or trehalose exhibited 0% membrane integrity, compared with 98% in cells loaded with trehalose and expressing AfrLEA2 or AfrLEA3m; surprisingly, AfrLEA3m without trehalose conferred 94% protection. Cell proliferation across 7 d showed an 18-fold increase for cells dried with AfrLEA3m and trehalose, compared with 27-fold for nondried controls. LEA proteins dramatically enhance desiccation tolerance in mammalian cells and offer the opportunity for engineering biostability in the dried state. PMID:23185012

  8. Design and development of desiccant seed dryer with airflow inversion and recirculation.

    PubMed

    Gill, R S; Singh, Sukhmeet; Singh, Parm Pal

    2014-11-01

    A desiccant seed dryer has been developed to dry seed in deep bed at safe temperatures for good shelf life and germination. The dryer consists of two chambers viz., air conditioning control unit and seed drying chamber. It operates in seed drying mode and desiccant regeneration mode. It has provision for recirculation of the drying air to minimise the moisture removal from drying air. Also, it has provision of airflow inversion through deep seed bed for uniform drying. Moisture removal from drying air has been done using silica gel desiccant. Chilly 'Punjab Surakh', Chilly 'Punjab Guchhedaar', Paddy, Coriander, Fenugreek and Radish seeds was dried with hot air at 38 °C from initial moisture content of 26.9 to 5 % (wb) in 2 h, 46.52 to 4.19 % (wb) in 4.25 h, 13.3 to 2.61 % (wb) in 4 h, 13.4 to 10.08 % (wb) in 3 h, 12.4 to 8.22 % (wb) in 4¼ h and 10.6 to 6.08 % (wb) in 4 h respectively. The statistical analysis based on paired t-test showed that seed drying in this dryer has no adverse effect on seed germination. PMID:26396340

  9. Taken to the limit--Is desiccation stress causing precocious encystment of trematode parasites in snails?

    PubMed

    O'Dwyer, Katie; Poulin, Robert

    2015-12-01

    When hosts experience environmental stress, the quantity and quality of resources they provide for parasites may be diminished, and host longevity may be decreased. Under stress, parasites may adopt alternative strategies to avoid fitness reductions. Trematode parasites typically have complex life cycles, involving asexual reproduction in a gastropod first intermediate host. A rare phenomenon, briefly mentioned in the literature, and termed 'precocious encystment' involves the next stage in the parasites' life cycle (metacercarial cyst) forming within the preceding stage (redia), while still inside the snail. In the trematode Parorchis sp. NZ using rocky shore snails exposed to long periods outside water, we hypothesised that this might be an adaptive strategy against desiccation, preventing parasite emergence from the snail. To test this, we first investigated the effect of prolonged desiccation on the survival of two species of high intertidal snails. Secondly, we measured the reproductive output (cercarial production) of the parasite under wet and dry conditions. Finally, we quantified the influence of desiccation stress on the occurrence of precocious encystment. Snail mortality was higher under dry conditions, indicating stress, and it was somewhat exacerbated for infected snails. Parasite reproductive output differed between wet and dry conditions, with parasites of snails kept in dry conditions producing more cercariae when placed in water. Little variation was observed in the occurrence of precocious encystment, although some subtle patterns emerged. Given the stresses associated with living in high intertidal environments, we discuss precocious encystment as a possible stress response in this trematode parasite. PMID:26344863

  10. Arabinose-rich polymers as an evolutionary strategy to plasticize resurrection plant cell walls against desiccation.

    PubMed

    Moore, John P; Nguema-Ona, Eric E; Vicré-Gibouin, Mäite; Sørensen, Iben; Willats, William G T; Driouich, Azeddine; Farrant, Jill M

    2013-03-01

    A variety of Southern African resurrection plants were surveyed using high-throughput cell wall profiling tools. Species evaluated were the dicotyledons, Myrothamnus flabellifolia and Craterostigma plantagineum; the monocotyledons, Xerophyta viscosa, Xerophyta schlecterii, Xerophyta humilis and the resurrection grass Eragrostis nindensis, as well as a pteridophyte, the resurrection fern, Mohria caffrorum. Comparisons were made between hydrated and desiccated leaf and frond material, with respect to cell wall composition and polymer abundance, using monosaccharide composition analysis, FT-IR spectroscopy and comprehensive microarray polymer profiling in combination with multivariate data analysis. The data obtained suggest that three main functional strategies appear to have evolved to prepare plant cell walls for desiccation. Arabinan-rich pectin and arabinogalactan proteins are found in the resurrection fern M. caffrorum and the basal angiosperm M. flabellifolia where they appear to act as 'pectic plasticizers'. Dicotyledons with pectin-rich walls, such as C. plantagineum, seem to use inducible mechanisms which consist of up-regulating wall proteins and osmoprotectants. The hemicellulose-rich walls of the grass-like Xerophyta spp. and the resurrection grass E. nindensis were found to contain highly arabinosylated xylans and arabinogalactan proteins. These data support a general mechanism of 'plasticising' the cell walls of resurrection plants to desiccation and implicate arabinose-rich polymers (pectin-arabinans, arabinogalactan proteins and arabinoxylans) as the major contributors in ensuring flexibility is maintained and rehydration is facilitated in these plants.

  11. Polypropylene Fiber Amendments to Alleviate Initiation and Evolution of Desiccation Cracks in Bentonite Liners

    NASA Astrophysics Data System (ADS)

    Tuller, M.; Gebrenegus, T. B.

    2009-12-01

    Sodium saturated bentonite is a major constituent of compacted and geosynthetic liners and covers for hydraulic isolation of hazardous waste, playing a crucial role in protecting groundwater and other environmental resources from harmful landfill leachates. Due to favorable hydraulic properties (i.e., low permeability), large surface area and associated adsorption capacity for particular contaminants, and relative abundance and low cost, bentonite is the material of choice in many modern waste containment systems. However, long-term interactions between bentonite and waste leachate and exposure of bentonite to desiccative conditions may significantly deteriorate liner or cover performance and ultimately lead to failure of containment systems. In the presented study, the potential usefulness of polypropylene fiber amendments for preventing initiation and evolution of desiccation cracks, while maintaining acceptably low permeability under saturated conditions was investigated. Well-controlled desiccation experiments were conducted using initially saturated bentonite-sand mixtures that contained varying amounts of polypropylene fibers. Initiation and evolution of surface cracks were observed by means of X-Ray Computed Tomography (CT). Advanced image analysis techniques were employed to characterize and quantify 2-D and 3-D features of the evolving crack networks. Potential negative effects of employed additives on saturated hydraulic conductivity were determined with fully-automated Flexible Wall Permeametry (FWP).

  12. An easily reversible structural change underlies mechanisms enabling desert crust cyanobacteria to survive desiccation.

    PubMed

    Bar-Eyal, Leeat; Eisenberg, Ido; Faust, Adam; Raanan, Hagai; Nevo, Reinat; Rappaport, Fabrice; Krieger-Liszkay, Anja; Sétif, Pierre; Thurotte, Adrien; Reich, Ziv; Kaplan, Aaron; Ohad, Itzhak; Paltiel, Yossi; Keren, Nir

    2015-10-01

    Biological desert sand crusts are the foundation of desert ecosystems, stabilizing the sands and allowing colonization by higher order organisms. The first colonizers of the desert sands are cyanobacteria. Facing the harsh conditions of the desert, these organisms must withstand frequent desiccation-hydration cycles, combined with high light intensities. Here, we characterize structural and functional modifications to the photosynthetic apparatus that enable a cyanobacterium, Leptolyngbya sp., to thrive under these conditions. Using multiple in vivo spectroscopic and imaging techniques, we identified two complementary mechanisms for dissipating absorbed energy in the desiccated state. The first mechanism involves the reorganization of the phycobilisome antenna system, increasing excitonic coupling between antenna components. This provides better energy dissipation in the antenna rather than directed exciton transfer to the reaction center. The second mechanism is driven by constriction of the thylakoid lumen which limits diffusion of plastocyanin to P700. The accumulation of P700(+) not only prevents light-induced charge separation but also efficiently quenches excitation energy. These protection mechanisms employ existing components of the photosynthetic apparatus, forming two distinct functional modes. Small changes in the structure of the thylakoid membranes are sufficient for quenching of all absorbed energy in the desiccated state, protecting the photosynthetic apparatus from photoinhibitory damage. These changes can be easily reversed upon rehydration, returning the system to its high photosynthetic quantum efficiency.

  13. Understanding desiccation tolerance using the resurrection plant Boea hygrometrica as a model system

    PubMed Central

    Mitra, Jayeeta; Xu, Guanghui; Wang, Bo; Li, Meijing; Deng, Xin

    2013-01-01

    Vegetative tissues of Boea hygrometrica, a member of the Gesneriaceae family, can tolerate severe water loss to desiccated state and fully recover upon rehydration. Unlike many other so called “resurrection plants,” the detached leaves of B. hygrometrica also possess the same level of capacity for desiccation tolerance (DT) as that of whole plant. B. hygrometrica is distributed widely from the tropics to northern temperate regions in East Asia and grows vigorously in areas around limestone rocks, where dehydration occurs frequently, rapidly, and profoundly. The properties of detached B. hygrometrica leaves and relative ease of culture have made it a useful system to study the adaptive mechanisms of DT. Extensive studies have been conducted to identify the physiological, cellular, and molecular mechanisms underlying DT in the last decade, including specific responses to water stress, such as cell wall folding and pigment-protein complex stabilizing in desiccated leaves. In this review, the insight into the structural, physiological, and biochemical, and molecular alterations that accompany the acquisition of DT in B. hygrometrica is described. Finally a future perspective is proposed, with an emphasis on the emerging regulatory roles of retroelements and histone modifications in the acquisition of DT, and the need of establishment of genome sequence database and high throughput techniques to identify novel regulators for fully understanding of the matrix of DT. PMID:24273545

  14. Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thaliana.

    PubMed

    González-Morales, Sandra Isabel; Chávez-Montes, Ricardo A; Hayano-Kanashiro, Corina; Alejo-Jacuinde, Gerardo; Rico-Cambron, Thelma Y; de Folter, Stefan; Herrera-Estrella, Luis

    2016-08-30

    Desiccation tolerance (DT) is a remarkable process that allows seeds in the dry state to remain viable for long periods of time that in some instances exceed 1,000 y. It has been postulated that seed DT evolved by rewiring the regulatory and signaling networks that controlled vegetative DT, which itself emerged as a crucial adaptive trait of early land plants. Understanding the networks that regulate seed desiccation tolerance in model plant systems would provide the tools to understand an evolutionary process that played a crucial role in the diversification of flowering plants. In this work, we used an integrated approach that included genomics, bioinformatics, metabolomics, and molecular genetics to identify and validate molecular networks that control the acquisition of DT in Arabidopsis seeds. Two DT-specific transcriptional subnetworks were identified related to storage of reserve compounds and cellular protection mechanisms that act downstream of the embryo development master regulators LEAFY COTYLEDON 1 and 2, FUSCA 3, and ABSCICIC ACID INSENSITIVE 3. Among the transcription factors identified as major nodes in the DT regulatory subnetworks, PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-intolerant mutant background to play an important role in seed DT. Additionally, we found that constitutive expression of PLATZ1 in WT plants confers partial DT in vegetative tissues. PMID:27551092

  15. Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thaliana

    PubMed Central

    González-Morales, Sandra Isabel; Chávez-Montes, Ricardo A.; Hayano-Kanashiro, Corina; Alejo-Jacuinde, Gerardo; Rico-Cambron, Thelma Y.; de Folter, Stefan; Herrera-Estrella, Luis

    2016-01-01

    Desiccation tolerance (DT) is a remarkable process that allows seeds in the dry state to remain viable for long periods of time that in some instances exceed 1,000 y. It has been postulated that seed DT evolved by rewiring the regulatory and signaling networks that controlled vegetative DT, which itself emerged as a crucial adaptive trait of early land plants. Understanding the networks that regulate seed desiccation tolerance in model plant systems would provide the tools to understand an evolutionary process that played a crucial role in the diversification of flowering plants. In this work, we used an integrated approach that included genomics, bioinformatics, metabolomics, and molecular genetics to identify and validate molecular networks that control the acquisition of DT in Arabidopsis seeds. Two DT-specific transcriptional subnetworks were identified related to storage of reserve compounds and cellular protection mechanisms that act downstream of the embryo development master regulators LEAFY COTYLEDON 1 and 2, FUSCA 3, and ABSCICIC ACID INSENSITIVE 3. Among the transcription factors identified as major nodes in the DT regulatory subnetworks, PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccation-intolerant mutant background to play an important role in seed DT. Additionally, we found that constitutive expression of PLATZ1 in WT plants confers partial DT in vegetative tissues. PMID:27551092

  16. Effects of Porous Medium Heterogeneity on Vadose Zone Desiccation: Intermediate-scale Laboratory Experiments and Simulations

    SciTech Connect

    Oostrom, Martinus; Freedman, Vicky L.; Wietsma, Thomas W.; Dane, Jacob H.; Truex, Michael J.

    2012-11-01

    Soil desiccation (drying), involving water evaporation induced by dry gas injection, is a potentially robust vadose zone remediation process to limit contaminant transport through the vadose zone. A series of four intermediate-scale flow cell experiments was conducted in homogeneous and simple layered heterogeneous porous medium systems to investigate the effects of heterogeneity on desiccation of unsaturated porous media. The permeability ratios of porous medium layers ranged from about five to almost two orders of magnitude. The insulated flow cell was equipped with twenty humidity and temperature sensors and a dual-energy gamma system was used to determine water saturations at various times. The multiphase code STOMP was used to simulate the desiccation process. Results show that injected dry gas flowed predominantly in the higher permeability layer and delayed water removal from the lower permeability material. For the configurations tested, water vapor diffusion from the lower to the higher permeability zone was considerable over the duration of the experiments, resulting in much larger relative humidity values of the outgoing air than based on permeability ratios alone. Acceptable numerical matches with the experimental data were obtained when an extension of the saturation-capillary pressure relation below the residual water saturation was used. The agreements between numerical and experimental results suggest that the correct physics are implemented in the simulator and that the thermal and hydraulic properties of the porous media, flow cell wall and insulation materials were properly represented.

  17. Re-induction of desiccation tolerance after germination of Cedrela fissilis Vell. seeds.

    PubMed

    Masetto, Tathiana E; Faria, Jose M; Fraiz, Ana C R

    2014-09-01

    This work aimed to characterize the re-induction of desiccation tolerance (DT) in germinated seeds, using polyethylene glycol (PEG 8000). Cell changes were investigated through cytological assays (cell viability and transmission electronic microscopy) as well as DNA integrity during loss and re-establishment of DT. The loss of DT was characterized by drying germinated seeds with different radicle lengths (1, 2, 3, 4 and 5 mm) in silica gel, decreasing the moisture content to ten percentage points intervals, followed by pre-humidification (100% RH / 24 h) and rehydration. To re-induce DT, germinated seeds were treated for 72 h with PEG (-2.04 MPa) and PEG (-2.04 MPa) + ABA (100 µM) before dehydration. Germinated seeds did not tolerate desiccation to 10% moisture content, irrespectively of the radicle length. However, when incubated in PEG, those with 1 and 2 mm long radicle attained 71% and 29% survival, respectively. The PEG+ABA treatment was efficient to re-establish DT in seeds with 1 mm long radicles (100% survival). The ultrastructural assays of the cells of germinated seeds with 2 and 5 mm length confirmed the obtained physiological results. Germinated seeds of C. fissilis constitute a useful tool for desiccation tolerance investigations.

  18. Extreme resistance to desiccation in overwintering larvae of the gall fly Eurosta solidaginis (Diptera, tephritidae).

    PubMed

    Ramløv, H; Lee, R E

    2000-02-01

    During winter, larvae of the goldenrod gall fly Eurosta solidaginis are exposed for extended periods to severe low ambient temperatures and low humidities within plant galls. The resistance of these larvae to desiccation at various temperatures and humidities, the transition (critical) temperature, and the effects of treatment with organic solvents on the larval rates of water loss and on changes in osmolality during desiccation were examined. The water loss rates of the flesh fly Sarcophaga crassipalpis under desiccating conditions were also measured. The water permeability of the cuticle of E. solidaginis larvae was very low (0.038 microgram h(-1 )cm(-2 )Pa(-1) at 20 C and 4% relative humidity) compared with that of larvae of other species. The value for E. solidaginis is equivalent to that of the very drought-resistant larvae of the tenebrionid beetle Tenebrio molitor (0.038 microgram h(-1)cm(-2)Pa(-1) at 30 C). In contrast, the permeability of larvae of the flesh fly Sarcophaga crassipalpis at 20 C and 4 % relative humidity was 0.331 microgram h(-1) cm(-2)Pa(-1). The thermal dependence of the cuticular permeability increased with temperature by approximately 0.0010 +microgram h(-1) cm(-2)Pa(-1) C(-1) in the interval between 4 and 40 C. At the transition temperature of 40 C, the thermal dependence of the permeability increased abruptly to 0.0400 microgram h(-1)cm(-2)Pa(-1) C(-1). Larvae treated with hexane and acetone remained remarkably resistant to water loss. However, treatment with chloroform:methanol increased the water loss rate approximately 25-fold. During desiccation at 4 C and 4% relative humidity for 21 days, E. solidaginis larvae showed a mass loss of 18.5+/-4.4 % (mean +/- s.e.m., N=6). Animals dried under the same conditions over the same period showed a haemolymph osmolality of 851+/-75 mosmol kg(-1) (N=4). Larvae freshly removed from the galls showed a haemolymph osmolality of 918+/-67 mosmol kg(-1)(N=3). A higher osmolality in the dried compared

  19. Wheat seedlings as a model to understand desiccation tolerance and sensitivity.

    PubMed

    Farrant, Jill M.; Bailly, Christophe; Leymarie, Juliette; Hamman, Brigitte; Côme, Daniel; Corbineau, Françoise

    2004-04-01

    The coleoptiles of wheat (Triticum aestivum L.) seedlings of cultivar Trémie are desiccation tolerant when 3 days old, although the roots are not. Cutting some of the coleoptiles open prior to dehydration rapidly increased the drying rate. This rendered the coleoptiles sensitive to desiccation, providing a useful model with which to study desiccation tolerance. Both sensitive and tolerant seedlings were dehydrated to 0.3 g H(2)O g(-1) dry mass (g.g) and thereafter rehydrated. Sensitive tissues accr- ued the lipid peroxidation products H(2)O(2)and MDA, and substantial subcellular damage was evident in dry tissues. H(2)O(2) and MDA accumulated slightly only in dry tolerant coleoptiles and no subcellular damage was evident. The activity of antioxidant enzymes glutathione reductase (EC1.6.2.4), superoxide dismutase (EC 1.14.1.1) and catalase (EC 1.11.1.6) increased on drying in both tolerant and sensitive tissues, but were sustained on rehydration in only the tolerant tissues. It is proposed that free radical damage sustained during rapid drying exceeded the ameliorating capacity of antioxidant systems, allowed accrual of lethal subcellular damage. Slow drying enabled sufficient detoxification by antioxidants to minimize damage and allow tolerance to drying. Three LEA- (p11 and Asp 52) and dehydrin- (XV8) like proteins were detected by western blots in tolerant coleoptiles dried to 3.0 g.g and below. Only one (Asp 52) was induced at low water content in rapidly dried sensitive coleoptiles. None were present in root tissues. XV8 RNA (northern analyses) was induced on drying only in tolerant coleoptiles and correlated with protein expression. These stress-putative protein protectants (and XV8 transcripts) appear to be down-regulated during germination but wheat seedlings temporarily retain the ability to reproduce them if drying is slow. Sucrose accumulation during dehydration was similar for both sensitive and tolerant tissues, suggesting that this sugar has little

  20. Airway cooling and mucosal injury during cold weather exercise.

    PubMed

    Davis, M S; Lockard, A J; Marlin, D J; Freed, A N

    2002-09-01

    In human subjects that exercise strenuously in cold weather, there is evidence that hyperventilation with cold air leads to peripheral airway cooling, desiccation and mucosal injury. Our hypothesis was that hyperventilation with cold air can result in penetration of unconditioned air (air that is not completely warmed and humidified) into the peripheral airways of exercising horses, resulting in peripheral airway mucosal injury. To test this hypothesis, a thermister-tipped catheter was inserted through the midcervical trachea and advanced into a sublobar bronchus in three horses that cantered on a treadmill at 6.6 m/s while breathing cold (5 degrees C) air. The mean (+/- s.e.) intra-airway temperature during cantering was 33.3 +/- 0.4 degrees C, a value comparable to the bronchial lumen temperatures measured in man during maximal exercise while breathing subfreezing dry air. In a second experiment, 6 fit Thoroughbred racehorses with satisfactory performance were used to determine whether strenuous exercise in cold conditions can produce airway injury. Horses were assigned to Exercise (E) or Control (C) groups in a random crossover design. Samples of bronchoalveolar lavage fluid (BALF) in the E treatment were recovered within 30 min of galloping exercise in 4 degrees C, 100% relative humidity (E), while in C BALF samples were obtained when the horses had not performed any exercise for at least 48 h prior. Ciliated epithelial cells in BALF were higher in E than in the C treatment. Similar results have been found in human athletes and laboratory animal models of cold weather exercise. These results support the hypothesis that, similar to man, horses that exercise in cold weather experience peripheral airway mucosal injury due to the penetration of unconditioned air. Furthermore, these results suggest that airway cooling and desiccation may be a factor in airway inflammation commonly found in equine athletes.

  1. Effect of insect cadaver desiccation and soil water potential during rehydration on entomopathogenic nematode (Rhabditida: Steinernematidae and Heterorhabditidae) production and virulence.

    PubMed

    Spence, K O; Stevens, G N; Arimoto, H; Ruiz-Vega, J; Kaya, H K; Lewis, E E

    2011-02-01

    We examined the influence of insect cadaver desiccation on the virulence and production of entomopathogenic nematodes (EPNs), common natural enemies of many soil-dwelling insects. EPNs are often used in biological control, and we investigated the feasibility of applying EPNs within desiccated insect cadavers. Desiccation studies were conducted using the factitious host, Galleria mellonella (Lepidoptera: Pyralidae, wax moth larvae) and three EPN species (Heterorhabditis bacteriophora 'HB1', Steinernema carpocapsae 'All', and Steinernema riobrave). Weights of individual insect cadavers were tracked daily during the desiccation process, and cohorts were placed into emergence traps when average mass losses reached 50%, 60%, and 70% levels. We tracked the proportion of insect cadavers producing infective juveniles (IJs), the number and virulence of IJs produced from desiccated insect cadavers, and the influence of soil water potentials on IJ production of desiccated insect cadavers. We observed apparent differences in the desiccation rate of the insect cadavers among the three species, as well as apparent differences among the three species in both the proportion of insect cadavers producing IJs and IJ production per insect cadaver. Exposure of desiccated insect cadavers to water potentials greater than -2.75 kPa stimulated IJ emergence. Among the nematode species examined, H. bacteriophora exhibited lower proportions of desiccated insect cadavers producing IJs than the other two species. Desiccation significantly reduced the number of IJs produced from insect cadavers. At the 60% mass loss level, however, desiccated insect cadavers from each of the three species successfully produced IJs when exposed to moist sand, suggesting that insect cadaver desiccation may be a useful approach for biological control of soil insect pests.

  2. Solar heating and cooling system installed at Leavenworth, Kansas. Final report

    SciTech Connect

    Perkins, R. M.

    1980-06-01

    The solar heating and cooling system installed at the headquarters of Citizens Mutual Savings Association in Leavenworth, Kansas, is described in detail. The project is part of the U.S. Department of Energy's solar demonstration program and became operational in March, 1979. The designer was TEC, Inc. Consulting Engineers, Kansas City, Missouri and contractor was Norris Brothers, Inc., Lawrence, Kansas. The solar system is expected to furnish 90 percent of the overall heating load, 70 percent of the cooling load and 100 percent of the domestic hot water load. The building has two floors with a total of 12,000 square feet gross area. The system has 120 flat-plate liquid solar panels with a net area of 2200 square feet. Five, 3-ton Arkla solar assisted absorption units provide the cooling, in conjunction with a 3000 gallon chilled water storage tank. Two, 3000 gallon storage tanks are provided with one designated for summer use, whereas both tanks are utilized during winter.

  3. High Efficiency Liquid-Desiccant Regenerator for Air Conditioning and Industrial Drying

    SciTech Connect

    Andrew Lowenstein

    2005-12-19

    Over 2 quads of fossil fuels are used each year for moisture removal. This includes industrial and agricultural processes where feedstocks and final products must be dried, as well as comfort conditioning of indoor spaces where the control of humidity is essential to maintaining healthy, productive and comfortable working conditions. Desiccants, materials that have a high affinity for water vapor, can greatly reduce energy use for both drying and dehumidification. An opportunity exists to greatly improve the competitiveness of advanced liquid-desiccant systems by increasing the efficiency of their regenerators. It is common practice within the chemical process industry to use multiple stage boilers to improve the efficiency of thermal separation processes. The energy needed to regenerate a liquid desiccant, which is a thermal separation process, can also be reduced by using a multiple stage boiler. In this project, a two-stage regenerator was developed in which the first stage is a boiler and the second stage is a scavenging-air regenerator. The only energy input to this regenerator is the natural gas that fires the boiler. The steam produced in the boiler provides the thermal energy to run the second-stage scavenging-air regenerator. This two-stage regenerator is referred to as a 1?-effect regenerator. A model of the high-temperature stage of a 1?-effect regenerator for liquid desiccants was designed, built and successfully tested. At nominal operating conditions (i.e., 2.35 gpm of 36% lithium chloride solution, 307,000 Btu/h firing rate), the boiler removed 153 lb/h of water from the desiccant at a gas-based efficiency of 52.9 % (which corresponds to a COP of 0.95 when a scavenging-air regenerator is added). The steam leaving the boiler, when condensed, had a solids concentration of less than 10 ppm. This low level of solids in the condensate places an upper bound of about 6 lb per year for desiccant loss from the regenerator. This low loss will not create

  4. Influence of Protoplasmic Water Loss on the Control of Protein Synthesis in the Desiccation-Tolerant Moss Tortula ruralis 1

    PubMed Central

    Oliver, Melvin J.

    1991-01-01

    Desiccation tolerance of the moss Tortula ruralis is characterized by a desiccation-induced change in gene expression that becomes evident upon rehydration. As reported earlier, this change in gene expression is apparently brought about by a change in the control of translation and does not include a major shift in mRNA abundance. A full qualitative and quantitative analysis of the alteration in gene expression, which is characterized by the loss of (or greater than fivefold decrease in) the synthesis of 25 hydration (h) proteins and initiation (or greater than fivefold increase) of the synthesis of 74 rehydration (r) proteins, is given in this report. Exposure to a desiccating atmosphere, for times that result in varying levels of water loss, enabled the determination that the control of synthesis of r proteins is different from the control of synthesis of h proteins. The r and h protein synthesis responses are internally coordinate, however. Similarly, the return to normal levels of h protein synthesis differs from that of the r proteins. The return to normal synthetic levels for all h proteins is synchronous, but the rate of loss of r protein synthesis varies with each individual r protein. Run-off translation of polysomes isolated from gametophytes during the drying phase demonstrates that there are no novel mRNAs recruited and no particular mRNA is favored for translation during desiccation. These findings add credence to the argument that translational control is the major component of the desiccation-induced alteration in gene expression in this plant, as discussed. Aspects of the response of protein synthesis to desiccation are consistent with the hypothesis that T. ruralis exhibits a repair-based mechanism of desiccation tolerance. ImagesFigure 2Figure 3Figure 5Figure 6Figure 7 PMID:16668577

  5. LEA polypeptide profiling of recalcitrant and orthodox legume seeds reveals ABI3-regulated LEA protein abundance linked to desiccation tolerance.

    PubMed

    Delahaie, Julien; Hundertmark, Michaela; Bove, Jérôme; Leprince, Olivier; Rogniaux, Hélène; Buitink, Julia

    2013-11-01

    In contrast to orthodox seeds that acquire desiccation tolerance during maturation, recalcitrant seeds are unable to survive drying. These desiccation-sensitive seeds constitute an interesting model for comparative analysis with phylogenetically close species that are desiccation tolerant. Considering the importance of LEA (late embryogenesis abundant) proteins as protective molecules both in drought and in desiccation tolerance, the heat-stable proteome was characterized in cotyledons of the legume Castanospermum australe and it was compared with that of the orthodox model legume Medicago truncatula. RNA sequencing identified transcripts of 16 homologues out of 17 LEA genes for which polypeptides are detected in M. truncatula seeds. It is shown that for 12 LEA genes, polypeptides were either absent or strongly reduced in C. australe cotyledons compared with M. truncatula seeds. Instead, osmotically responsive, non-seed-specific dehydrins accumulated to high levels in the recalcitrant cotyledons compared with orthodox seeds. Next, M. truncatula mutants of the abscisic acid insensitive3 (ABI3) gene were characterized. Mature Mtabi3 seeds were found to be desiccation sensitive when dried below a critical water content of 0.4 g H2O g DW(-1). Characterization of the LEA proteome of the Mtabi3 seeds revealed a subset of LEA proteins with severely reduced abundance that were also found to be reduced or absent in C. australe cotyledons. Transcripts of these genes were indeed shown to be ABI3 responsive. The results highlight those LEA proteins that are critical to desiccation tolerance and suggest that comparable regulatory pathways responsible for their accumulation are missing in both desiccation-sensitive genotypes, revealing new insights into the mechanistic basis of the recalcitrant trait in seeds.

  6. Gas turbine cooling system

    SciTech Connect

    Bancalari, Eduardo E.

    2001-01-01

    A gas turbine engine (10) having a closed-loop cooling circuit (39) for transferring heat from the hot turbine section (16) to the compressed air (24) produced by the compressor section (12). The closed-loop cooling system (39) includes a heat exchanger (40) disposed in the flow path of the compressed air (24) between the outlet of the compressor section (12) and the inlet of the combustor (14). A cooling fluid (50) may be driven by a pump (52) located outside of the engine casing (53) or a pump (54) mounted on the rotor shaft (17). The cooling circuit (39) may include an orifice (60) for causing the cooling fluid (50) to change from a liquid state to a gaseous state, thereby increasing the heat transfer capacity of the cooling circuit (39).

  7. Solar heating and cooling

    NASA Technical Reports Server (NTRS)

    Bartera, R. E.

    1978-01-01

    To emphasize energy conservation and low cost energy, the systems of solar heating and cooling are analyzed and compared with fossil fuel systems. The application of solar heating and cooling systems for industrial and domestic use are discussed. Topics of discussion include: solar collectors; space heating; pools and spas; domestic hot water; industrial heat less than 200 F; space cooling; industrial steam; and initial systems cost. A question and answer period is generated which closes out the discussion.

  8. The cooling of particle beams

    SciTech Connect

    Sessler, A.M.

    1994-10-01

    A review is given of the various methods which can be employed for cooling particle beams. These methods include radiation damping, stimulated radiation damping, ionization cooling, stochastic cooling, electron cooling, laser cooling, and laser cooling with beam coupling. Laser Cooling has provided beams of the lowest temperatures, namely 1 mK, but only for ions and only for the longitudinal temperature. Recent theoretical work has suggested how laser cooling, with the coupling of beam motion, can be used to reduce the ion beam temperature in all three directions. The majority of this paper is devoted to describing laser cooling and laser cooling with beam coupling.

  9. Semioptimal practicable algorithmic cooling

    NASA Astrophysics Data System (ADS)

    Elias, Yuval; Mor, Tal; Weinstein, Yossi

    2011-04-01

    Algorithmic cooling (AC) of spins applies entropy manipulation algorithms in open spin systems in order to cool spins far beyond Shannon’s entropy bound. Algorithmic cooling of nuclear spins was demonstrated experimentally and may contribute to nuclear magnetic resonance spectroscopy. Several cooling algorithms were suggested in recent years, including practicable algorithmic cooling (PAC) and exhaustive AC. Practicable algorithms have simple implementations, yet their level of cooling is far from optimal; exhaustive algorithms, on the other hand, cool much better, and some even reach (asymptotically) an optimal level of cooling, but they are not practicable. We introduce here semioptimal practicable AC (SOPAC), wherein a few cycles (typically two to six) are performed at each recursive level. Two classes of SOPAC algorithms are proposed and analyzed. Both attain cooling levels significantly better than PAC and are much more efficient than the exhaustive algorithms. These algorithms are shown to bridge the gap between PAC and exhaustive AC. In addition, we calculated the number of spins required by SOPAC in order to purify qubits for quantum computation. As few as 12 and 7 spins are required (in an ideal scenario) to yield a mildly pure spin (60% polarized) from initial polarizations of 1% and 10%, respectively. In the latter case, about five more spins are sufficient to produce a highly pure spin (99.99% polarized), which could be relevant for fault-tolerant quantum computing.

  10. Passive containment cooling system

    DOEpatents

    Conway, Lawrence E.; Stewart, William A.

    1991-01-01

    A containment cooling system utilizes a naturally induced air flow and a gravity flow of water over the containment shell which encloses a reactor core to cool reactor core decay heat in two stages. When core decay heat is greatest, the water and air flow combine to provide adequate evaporative cooling as heat from within the containment is transferred to the water flowing over the same. The water is heated by heat transfer and then evaporated and removed by the air flow. After an initial period of about three to four days when core decay heat is greatest, air flow alone is sufficient to cool the containment.

  11. High energy electron cooling

    SciTech Connect

    Parkhomchuk, V.

    1997-09-01

    High energy electron cooling requires a very cold electron beam. The questions of using electron cooling with and without a magnetic field are presented for discussion at this workshop. The electron cooling method was suggested by G. Budker in the middle sixties. The original idea of the electron cooling was published in 1966. The design activities for the NAP-M project was started in November 1971 and the first run using a proton beam occurred in September 1973. The first experiment with both electron and proton beams was started in May 1974. In this experiment good result was achieved very close to theoretical prediction for a usual two component plasma heat exchange.

  12. Body cooling after death.

    PubMed

    Kuehn, L A; Tikuisis, P; Livingstone, S; Limmer, R

    1980-09-01

    In the analyses of cases of death in cold air environments, it is often of interest to determine the time required for the body of the individual to cool to ambient temperature. Usually such determinations have been based on Newton's law of cooling. This paper describes a case history in which this technique was experimentally tested and consequently abandoned in favour of a more complex biophysical model which more accurately described the thermo-physical events inherent in body cooling. This model is recommended for determination of the times required for various body parts to cool to ambient environmental temperatures.

  13. Power electronics cooling apparatus

    SciTech Connect

    Sanger, Philip Albert; Lindberg, Frank A.; Garcen, Walter

    2000-01-01

    A semiconductor cooling arrangement wherein a semiconductor is affixed to a thermally and electrically conducting carrier such as by brazing. The coefficient of thermal expansion of the semiconductor and carrier are closely matched to one another so that during operation they will not be overstressed mechanically due to thermal cycling. Electrical connection is made to the semiconductor and carrier, and a porous metal heat exchanger is thermally connected to the carrier. The heat exchanger is positioned within an electrically insulating cooling assembly having cooling oil flowing therethrough. The arrangement is particularly well adapted for the cooling of high power switching elements in a power bridge.

  14. Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia

    PubMed Central

    Ma, Chao; Wang, Hong; Macnish, Andrew J; Estrada-Melo, Alejandro C; Lin, Jing; Chang, Youhong; Reid, Michael S; Jiang, Cai-Zhong

    2015-01-01

    The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia. PMID:26504577

  15. Synergistic interaction between the fungus Beauveria bassiana and desiccant dusts applied against poultry red mites (Dermanyssus gallinae).

    PubMed

    Steenberg, Tove; Kilpinen, Ole

    2014-04-01

    The poultry red mite, Dermanyssus gallinae, is a major pest in egg production, feeding on laying hens. Widely used non-chemical control methods include desiccant dusts, although their persistence under field conditions is often short. Entomopathogenic fungi may also hold potential for mite control, but these fungi often take several days to kill mites. Laboratory experiments were carried out to study the efficacy of 3 types of desiccant dusts, the fungus Beauveria bassiana and combinations of the two control agents against D. gallinae. There was significant synergistic interaction between each of the desiccant dusts and the fungus, with observed levels of mite mortality significantly higher than those expected for an additive effect (up to 38 % higher). Synergistic interaction between desiccant dust and fungus was found also when different application methods were used for the fungus and at different levels of relative humidity. Although increased levels of mortality were reached due to the synergistic interaction, the speed of lethal action was not influenced by combining the two components. The persistence of the control agents applied separately or in combination did not change over a period of 4 weeks. Overall, combinations of desiccant dusts and fungus conidia seem to hold considerable promise for future non-chemical control of poultry red mites. PMID:24253584

  16. Proteomic analysis of oil body membrane proteins accompanying the onset of desiccation phase during sunflower seed development.

    PubMed

    Thakur, Anita; Bhatla, Satish C

    2015-01-01

    A noteworthy metabolic signature accompanying oil body (OB) biogenesis during oilseed development is associated with the modulation of the oil body membranes proteins. Present work focuses on 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE)-based analysis of the temporal changes in the OB membrane proteins analyzed by LC-MS/MS accompanying the onset of desiccation (20-30 d after anthesis; DAA) in the developing seeds of sunflower (Helianthus annuus L.). Protein spots unique to 20-30 DAA stages were picked up from 2-D gels for identification and the identified proteins were categorized into 7 functional classes. These include proteins involved in energy metabolism, reactive oxygen scavenging, proteolysis and protein turnover, signaling, oleosin and oil body biogenesis-associated proteins, desiccation and cytoskeleton. At 30 DAA stage, exclusive expressions of enzymes belonging to energy metabolism, desiccation and cytoskeleton were evident which indicated an increase in the metabolic and enzymatic activity in the cells at this stage of seed development (seed filling). Increased expression of cruciferina-like protein and dehydrin at 30 DAA stage marks the onset of desiccation. The data has been analyzed and discussed to highlight desiccation stage-associated metabolic events during oilseed development.

  17. Proteomic analysis of oil body membrane proteins accompanying the onset of desiccation phase during sunflower seed development.

    PubMed

    Thakur, Anita; Bhatla, Satish C

    2015-01-01

    A noteworthy metabolic signature accompanying oil body (OB) biogenesis during oilseed development is associated with the modulation of the oil body membranes proteins. Present work focuses on 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE)-based analysis of the temporal changes in the OB membrane proteins analyzed by LC-MS/MS accompanying the onset of desiccation (20-30 d after anthesis; DAA) in the developing seeds of sunflower (Helianthus annuus L.). Protein spots unique to 20-30 DAA stages were picked up from 2-D gels for identification and the identified proteins were categorized into 7 functional classes. These include proteins involved in energy metabolism, reactive oxygen scavenging, proteolysis and protein turnover, signaling, oleosin and oil body biogenesis-associated proteins, desiccation and cytoskeleton. At 30 DAA stage, exclusive expressions of enzymes belonging to energy metabolism, desiccation and cytoskeleton were evident which indicated an increase in the metabolic and enzymatic activity in the cells at this stage of seed development (seed filling). Increased expression of cruciferina-like protein and dehydrin at 30 DAA stage marks the onset of desiccation. The data has been analyzed and discussed to highlight desiccation stage-associated metabolic events during oilseed development. PMID:26786011

  18. Proteomic analysis of oil body membrane proteins accompanying the onset of desiccation phase during sunflower seed development

    PubMed Central

    Thakur, Anita; Bhatla, Satish C

    2015-01-01

    A noteworthy metabolic signature accompanying oil body (OB) biogenesis during oilseed development is associated with the modulation of the oil body membranes proteins. Present work focuses on 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE)-based analysis of the temporal changes in the OB membrane proteins analyzed by LC-MS/MS accompanying the onset of desiccation (20–30 d after anthesis; DAA) in the developing seeds of sunflower (Helianthus annuus L.). Protein spots unique to 20–30 DAA stages were picked up from 2-D gels for identification and the identified proteins were categorized into 7 functional classes. These include proteins involved in energy metabolism, reactive oxygen scavenging, proteolysis and protein turnover, signaling, oleosin and oil body biogenesis-associated proteins, desiccation and cytoskeleton. At 30 DAA stage, exclusive expressions of enzymes belonging to energy metabolism, desiccation and cytoskeleton were evident which indicated an increase in the metabolic and enzymatic activity in the cells at this stage of seed development (seed filling). Increased expression of cruciferina-like protein and dehydrin at 30 DAA stage marks the onset of desiccation. The data has been analyzed and discussed to highlight desiccation stage-associated metabolic events during oilseed development. PMID:26786011

  19. Synergistic interaction between the fungus Beauveria bassiana and desiccant dusts applied against poultry red mites (Dermanyssus gallinae).

    PubMed

    Steenberg, Tove; Kilpinen, Ole

    2014-04-01

    The poultry red mite, Dermanyssus gallinae, is a major pest in egg production, feeding on laying hens. Widely used non-chemical control methods include desiccant dusts, although their persistence under field conditions is often short. Entomopathogenic fungi may also hold potential for mite control, but these fungi often take several days to kill mites. Laboratory experiments were carried out to study the efficacy of 3 types of desiccant dusts, the fungus Beauveria bassiana and combinations of the two control agents against D. gallinae. There was significant synergistic interaction between each of the desiccant dusts and the fungus, with observed levels of mite mortality significantly higher than those expected for an additive effect (up to 38 % higher). Synergistic interaction between desiccant dust and fungus was found also when different application methods were used for the fungus and at different levels of relative humidity. Although increased levels of mortality were reached due to the synergistic interaction, the speed of lethal action was not influenced by combining the two components. The persistence of the control agents applied separately or in combination did not change over a period of 4 weeks. Overall, combinations of desiccant dusts and fungus conidia seem to hold considerable promise for future non-chemical control of poultry red mites.

  20. Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia.

    PubMed

    Ma, Chao; Wang, Hong; Macnish, Andrew J; Estrada-Melo, Alejandro C; Lin, Jing; Chang, Youhong; Reid, Michael S; Jiang, Cai-Zhong

    2015-01-01

    The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia. PMID:26504577

  1. Comparative study of Saccharomyces cerevisiae wine strains to identify potential marker genes correlated to desiccation stress tolerance.

    PubMed

    Capece, Angela; Votta, Sonia; Guaragnella, Nicoletta; Zambuto, Marianna; Romaniello, Rossana; Romano, Patrizia

    2016-05-01

    The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its survival. The molecular mechanisms underlying cell stress response to desiccation have been mostly studied in laboratory and commercial yeast strains, but a growing interest is currently developing for indigenous yeast strains which represent a valuable and alternative source of genetic and molecular biodiversity to be exploited. In this work, a comparative study of different Saccharomyces cerevisiae indigenous wine strains, previously selected for their technological traits, has been carried out to identify potentially relevant genes involved in desiccation stress tolerance. Cell viability was evaluated along desiccation treatment and gene expression was analyzed by real-time PCR before and during the stress. Our data show that the observed differences in individual strain sensitivity to desiccation stress could be associated to specific gene expression over time. In particular, either the basal or the stress-induced mRNA levels of certain genes, such as HSP12, SSA3, TPS1, TPS2, CTT1 and SOD1, result tightly correlated to the strain survival advantage. This study provides a reliable and sensitive method to predict desiccation stress tolerance of indigenous wine yeast strains which could be preliminary to biotechnological applications.

  2. Comparative study of Saccharomyces cerevisiae wine strains to identify potential marker genes correlated to desiccation stress tolerance.

    PubMed

    Capece, Angela; Votta, Sonia; Guaragnella, Nicoletta; Zambuto, Marianna; Romaniello, Rossana; Romano, Patrizia

    2016-05-01

    The most diffused formulation of starter for winemaking is active dry yeast (ADY). ADYs production process is essentially characterized by air-drying stress, a combination of several stresses, including thermal, hyperosmotic and oxidative and cell capacity to counteract such multiple stresses will determine its survival. The molecular mechanisms underlying cell stress response to desiccation have been mostly studied in laboratory and commercial yeast strains, but a growing interest is currently developing for indigenous yeast strains which represent a valuable and alternative source of genetic and molecular biodiversity to be exploited. In this work, a comparative study of different Saccharomyces cerevisiae indigenous wine strains, previously selected for their technological traits, has been carried out to identify potentially relevant genes involved in desiccation stress tolerance. Cell viability was evaluated along desiccation treatment and gene expression was analyzed by real-time PCR before and during the stress. Our data show that the observed differences in individual strain sensitivity to desiccation stress could be associated to specific gene expression over time. In particular, either the basal or the stress-induced mRNA levels of certain genes, such as HSP12, SSA3, TPS1, TPS2, CTT1 and SOD1, result tightly correlated to the strain survival advantage. This study provides a reliable and sensitive method to predict desiccation stress tolerance of indigenous wine yeast strains which could be preliminary to biotechnological applications. PMID:26882930

  3. Liquid metal cooled nuclear reactors with passive cooling system

    DOEpatents

    Hunsbedt, Anstein; Fanning, Alan W.

    1991-01-01

    A liquid metal cooled nuclear reactor having a passive cooling system for removing residual heat resulting from fuel decay during reactor shutdown. The passive cooling system comprises a plurality of cooling medium flow circuits which cooperate to remove and carry heat away from the fuel core upon loss of the normal cooling flow circuit to areas external thereto.

  4. Mild desiccation rapidly increases freeze tolerance of the goldenrod gall fly, Eurosta solidaginis: evidence for drought-induced rapid cold-hardening.

    PubMed

    Levis, Nicholas A; Yi, Shu-Xia; Lee, Richard E

    2012-11-01

    Overwintering insects may experience extreme cold and desiccation stress. Both freezing and desiccation require cells to tolerate osmotic challenge as solutes become concentrated in the hemolymph. Not surprisingly, physiological responses to low temperature and desiccation share common features and may confer cross-tolerance against these stresses. Freeze-tolerant larvae of the goldenrod gall fly, Eurosta solidaginis (Diptera: Tephritidae), experience extremely dry and cold conditions in winter. To determine whether mild desiccation can improve freeze tolerance at organismal and cellular levels, we assessed survival, hemolymph osmolality and glycerol levels of control and desiccated larvae. Larvae that lost only 6-10% of their body mass, in as little as 6 h, had markedly higher levels of freeze tolerance. Mild, rapid desiccation increased freezing tolerance at -15°C in September-collected larvae (33.3±6.7 to 73.3±12%) and at -20°C in October-collected larvae (16.7±6.7 to 46.7±3.3%). Similarly, 6 h of desiccation improved in vivo survival by 17-43% in fat body, Malpighian tubule, salivary gland and tracheal cells at -20°C. Desiccation also enhanced intrinsic levels of cold tolerance in midgut cells frozen ex vivo (38.7±4.6 to 89.2±5.5%). Whereas hemolymph osmolality increased significantly with desiccation treatment from 544±16 to 720±26 mOsm, glycerol levels did not differ between control and desiccated groups. The rapidity with which a mild desiccation stress increased freeze tolerance closely resembles the rapid cold-hardening response, which occurs during brief sub-lethal chilling, and suggests that drought stress can induce rapid cold-hardening.

  5. Role of sigB and osmolytes in desiccation survival of Listeria monocytogenes in simulated food soils on the surface of food grade stainless steel.

    PubMed

    Huang, Yannan; Ells, Timothy C; Truelstrup Hansen, Lisbeth

    2015-04-01

    This research aimed to determine whether the SigB (σ(B)) regulon and osmolytes impact the survival of the foodborne pathogen, Listeria monocytogenes, during desiccation in simulated food soils with varying salt and nutrient contents on food grade stainless steel (SS) surfaces. L. monocytogenes 568 (Lm568, serotype 1/2a), its isogenic sigB mutant (ΔsigB) and the back-complemented ΔsigB were desiccated in BHI, TSB with 1% glucose (TSB-glu), peptone physiological saline (PPS) and minimal media (MM) for 21 days at 43% relative humidity (RH) and 15 °C on SS. The effect of food related osmolytes (proline, betaine and carnitine) on desiccation survival was studied by (a) pre-culturing strains in MM with an osmolyte followed by desiccation in MM and (b) by desiccating strains in MM with an osmolyte. Desiccation survival of L. monocytogenes was positively correlated to the nutrient and osmolyte concentrations in the desiccation substrates. Initial Lm568 levels of 8 Log(CFU/cm(2)) decreased by 0.9 Log(CFU/cm(2)) in BHI and 1.1-2.9 Log(CFU/cm(2)) in TSB-glu, PPS and MM after 21 days. Comparatively, the initial survival of ΔsigB was reduced in PS and MM, while no differences were observed among the three strains in BHI and TSB-glu. Pre-culture in osmolyte containing MM enhanced (p < 0.05) desiccation survival of all strains. Desiccation in osmolyte-containing MM improved desiccation survival of all strains, albeit the protection was less than that observed after pre-culture with the osmolytes. Complementation of the ΔsigB mutant restored the wildtype phenotype. In conclusion, this work shows the protective effect of osmolytes in desiccation survival of L. monocytogenes, while the σ(B) regulon only improved the initial survival in nutrient and osmolyte poor environments.

  6. Loss of fertilization potential of desiccated rhesus macaque spermatozoa following prolonged storage.

    PubMed

    Klooster, Katie L; Burruel, Victoria R; Meyers, Stuart A

    2011-06-01

    Desiccation provides a novel spermatozoal preservation technique because it eliminates the need to store spermatozoa in liquid nitrogen, resulting in decreased opportunities for cross-contamination of samples and lower costs of spermatozoal banking, storage, and shipping. The objective of this study was to desiccate rhesus macaque spermatozoa and to evaluate the fertility following storage. Semen from four male rhesus macaques (Macaca mulatta) were collected using electroejaculation, washed through a Percoll gradient, and resuspended to 100×10(6) spermatozoa/mL in a simple vacuum drying buffer containing the disaccharide trehalose (10mM HEPES, 5mM KCl, 65mMNaCl, 150mMtrehalose, 5.7% bovine serum albumin, BSA). Cells were desiccated in 50μl drops under vacuum (22inHg) at ambient temperature until the water content was less than 1g H(2)O/g dry weight. Initial motility was high (90-70%) and was reduced by desiccation (0%). Membrane integrity was investigated using the two fluorochromes, SYBR 14 and propidium iodide (PI, Molecular Probes, Inc.), with flow cytometry. After desiccation, 100% of the spermatozoa were stained red with PI indicating plasma membrane compromise. Samples were stored in air-tight polyvinyl plastic bags purged with N(2) gas for 10s and vacuum sealed. The samples were protected from light and either stored at room temperature (treatment 1) or at -80°C (treatment 2). Samples were rehydrated 7-10 days post desiccation in 150μl BWW containing 0.5% BSA and used for intracytoplasmic spermatozoa injection (ICSI) to compare fertilization and embryo development to freshly collected samples. For control embryos, one freshly ejaculated motile sperm with normal morphology was immobilized by scoring a small incision in the plasma membrane over the sperm tail before injecting into an oocyte. For the dried sperm treatments, one sperm with normal morphology was selected and scored before injecting into an oocyte. After injection, the embryos were

  7. Elastocaloric cooling: Stretch to actively cool

    NASA Astrophysics Data System (ADS)

    Ossmer, Hinnerk; Kohl, Manfred

    2016-10-01

    The elastocaloric effect can be exploited in solid-state cooling technologies as an alternative to conventional vapour compression. Now, an elastocaloric device based on the concept of active regeneration achieves a temperature lift of 15.3 K and efficiencies competitive with other caloric-based approaches.

  8. District cooling in Scandinavia

    SciTech Connect

    Andersson, B.

    1996-11-01

    This paper will present the status of the development of district cooling systems in Scandinavia over the last 5 years. It will describe the technologies used in the systems that have been constructed as well as the options considered in different locations. It will identify the drivers for the development of the cooling business to-date, and what future drivers for a continuing development of district cooling in Sweden. To-date, approximately 25 different cities of varying sizes have completed feasibility studies to determine if district cooling is an attractive option. In a survey, that was conducted by the Swedish District Heating Association, some 25 cities expected to have district cooling systems in place by the year 2000. In Sweden, district heating systems with hot water is very common. In many cases, it is simply an addition to the current service for the district heating company to also supply district cooling to the building owners. A parallel from this can be drawn to North America where district cooling systems now are developing rapidly. I am convinced that in these cities a district heating service will be added as a natural expansion of the district cooling company`s service.

  9. Cool Earth Solar

    ScienceCinema

    Lamkin, Rob; McIlroy, Andy; Swalwell, Eric; Rajan, Kish

    2016-07-12

    In a public-private partnership that takes full advantage of the Livermore Valley Open Campus (LVOC) for the first time, Sandia National Laboratories and Cool Earth Solar have signed an agreement that could make solar energy more affordable and accessible. In this piece, representatives from Sandia, Cool Earth Solar, and leaders in California government all discuss the unique partnership and its expected impact.

  10. Data center cooling method

    DOEpatents

    Chainer, Timothy J.; Dang, Hien P.; Parida, Pritish R.; Schultz, Mark D.; Sharma, Arun

    2015-08-11

    A method aspect for removing heat from a data center may use liquid coolant cooled without vapor compression refrigeration on a liquid cooled information technology equipment rack. The method may also include regulating liquid coolant flow to the data center through a range of liquid coolant flow values with a controller-apparatus based upon information technology equipment temperature threshold of the data center.

  11. Measure Guideline: Ventilation Cooling

    SciTech Connect

    Springer, D.; Dakin, B.; German, A.

    2012-04-01

    The purpose of this measure guideline on ventilation cooling is to provide information on a cost-effective solution for reducing cooling system energy and demand in homes located in hot-dry and cold-dry climates. This guideline provides a prescriptive approach that outlines qualification criteria, selection considerations, and design and installation procedures.

  12. Cool Earth Solar

    SciTech Connect

    Lamkin, Rob; McIlroy, Andy; Swalwell, Eric; Rajan, Kish

    2013-04-22

    In a public-private partnership that takes full advantage of the Livermore Valley Open Campus (LVOC) for the first time, Sandia National Laboratories and Cool Earth Solar have signed an agreement that could make solar energy more affordable and accessible. In this piece, representatives from Sandia, Cool Earth Solar, and leaders in California government all discuss the unique partnership and its expected impact.

  13. S'COOL Science

    ERIC Educational Resources Information Center

    Bryson, Linda

    2004-01-01

    This article describes one fifth grade's participation in in NASA's S'COOL (Students' Cloud Observations On-Line) Project, making cloud observations, reporting them online, exploring weather concepts, and gleaning some of the things involved in authentic scientific research. S?COOL is part of a real scientific study of the effect of clouds on…

  14. Why Cool Roofs?

    ScienceCinema

    Chu, Steven

    2016-07-12

    By installing a cool roof at DOE, the federal government and Secretary Chu are helping to educate families and businesses about the important energy and cost savings that can come with this simple, low-cost technology. Cool roofs have the potential to quickly and dramatically reduce global carbon emissions while saving money every month on consumers' electrical bills.

  15. DOAS, Radiant Cooling Revisited

    SciTech Connect

    Hastbacka, Mildred; Dieckmann, John; Bouza, Antonio

    2012-12-01

    The article discusses dedicated outdoor air systems (DOAS) and radiant cooling technologies. Both of these topics were covered in previous ASHRAE Journal columns. This article reviews the technologies and their increasing acceptance. The two steps that ASHRAE is taking to disseminate DOAS information to the design community, available energy savings and the market potential of radiant cooling systems are addressed as well.

  16. Why Cool Roofs?

    SciTech Connect

    Chu, Steven

    2010-01-01

    By installing a cool roof at DOE, the federal government and Secretary Chu are helping to educate families and businesses about the important energy and cost savings that can come with this simple, low-cost technology. Cool roofs have the potential to quickly and dramatically reduce global carbon emissions while saving money every month on consumers' electrical bills.

  17. A small stress protein acts synergistically with trehalose to confer desiccation tolerance on mammalian cells.

    PubMed

    Ma, Xiaocui; Jamil, Kamran; Macrae, Thomas H; Clegg, James S; Russell, Joseph M; Villeneuve, Tania S; Euloth, Michelle; Sun, Yu; Crowe, John H; Tablin, Fern; Oliver, Ann E

    2005-08-01

    The ability to desiccate mammalian cells while maintaining a high degree of viability would be very important in many areas of biological science, including tissue engineering, cell transplantation, and biosensor technologies. Certain proteins and sugars found in animals capable of surviving desiccation might aid this process. We report here that human embryonic kidney (293H) cells transfected with the gene for the stress protein p26 from Artemia and loaded with trehalose showed a sharp increase in survival during air-drying. Further, we find vacuum-drying greatly improved the ability of the cells to survive, and that the physical shape and structure of the cellular sample had a large influence on recovery following rehydration. Cells suspended in a rounded droplet survived desiccation markedly better than those spread as a thin film. Finally, we used alamarBlue to monitor cellular metabolism and Hema 3 to assess colony formation after vacuum-drying. AlamarBlue fluorescence indicated that the transfected 293H cells expressing p26 (E11'L) grew much better than the control 293H cells. In fact, immediate survival and colony formation in E11'L cells increased as much as 34-fold compared with control cells when the samples were dried to a water content of 0.2 g H2O/g dry weight, as measured by gravimetric analysis. These results indicate that p26 improves cell survival following drying and rehydration, and suggest that dry storage of mammalian cells is a likely possibility in the future. PMID:15963489

  18. Facing the Heat: Does Desiccation and Thermal Stress Explain Patterns of Orientation in an Intertidal Invertebrate?

    PubMed Central

    Fraser, Clarissa M. L.; Seebacher, Frank; Lathlean, Justin; Coleman, Ross A.

    2016-01-01

    A key challenge for ecologists is to quantify, explain and predict the ecology and behaviour of animals from knowledge of their basic physiology. Compared to our knowledge of many other types of distribution and behaviour, and how these are linked to individual function, we have a poor level of understanding of the causal basis for orientation behaviours. Most explanations for patterns of animal orientation assume that animals will modify their exposure to environmental factors by altering their orientation. We used a keystone grazer on rocky shores, the limpet Cellana tramoserica, to test this idea. Manipulative experiments were done to evaluate whether orientation during emersion affected limpet desiccation or body temperature. Body temperature was determined from infrared thermography, a technique that minimises disturbance to the test organism. No causal relationships were found between orientation and (i) level of desiccation and (ii) their body temperature. These results add to the growing knowledge that responses to desiccation and thermal stress may be less important in modifying the behaviour of intertidal organisms than previously supposed and that thermoregulation does not always reflect patterns of animal orientation. Much of what we understand about orientation comes from studies of animals able to modify orientation over very short time scales. Our data suggests that for animals whose location is less flexible, orientation decisions may have less to do with responses to environmental factors and more to do with structural habitat properties or intrinsic individual attributes. Therefore we suggest future studies into processes affecting orientation must include organisms with differing levels of behavioural plasticity. PMID:26959815

  19. Effect of different artificial tears against desiccation in cultured human epithelial cells

    PubMed Central

    Tost, Frank; Keiss, Ramona; Großjohann, Rico; Jürgens, Clemens; Giebel, Jürgen

    2012-01-01

    Summary Background A large number of artificial tears is widely used to treat dry eye symptoms. To test the efficacy of these drugs independent of individual parameters in vitro models are required. As described previously, we employed a reproducible in vitro cell culture system to evaluate the desiccation protection capability of some artificial tears. In the present paper data is presented of another set of pharmaceutical agents. Material/Methods Conjunctival epithelial cell line Chang 1-5c-4 (series 1) and the corneal cell line 2.040 pRSV-T (series 2) were cultured under standard conditions. Confluent cells were wetted for 20 min with artificial tears (Arufil® Uno, Arufil®, Lacrimal®, Lacophthal® sine, Siccaprotect®, Tears Again®, Vidisept® EDO, Vistil®, Wet Comod®) or PBS as a control. After exposure to a constant air flow for 0, 15, 30 and 45 minutes respectively, cells were incubated with the vital dye alamarBlue. Subsequently, absorption of the oxidised form of the dye was assessed using an ELISA-Reader. Results Cell best survival rates in series 1 after 15 min were found for Lacrimal® (0.89), Wet Comod® (0.84) compared to PBS (0.66) and in series 2 for Vidisept® EDO (0.57) and Lacrimal® (0.56) compared to PBS (0.01). After 45 min highest survival was seen in series 1 for Lacrimal® (0.46) and Lacophthal® sine (0.36) compared to PBS (0.33) and in series 2 for Lacrimal® (−0.06) and Arufil (−0.16) compared to PBS (−0.23). Conclusions Both cell lines tested showed different susceptibility towards desiccation and the artificial tears showed differences in preventing cells from desiccation. PMID:22534701

  20. Water cooled steam jet

    DOEpatents

    Wagner, Jr., Edward P.

    1999-01-01

    A water cooled steam jet for transferring fluid and preventing vapor lock, or vaporization of the fluid being transferred, has a venturi nozzle and a cooling jacket. The venturi nozzle produces a high velocity flow which creates a vacuum to draw fluid from a source of fluid. The venturi nozzle has a converging section connected to a source of steam, a diffuser section attached to an outlet and a throat portion disposed therebetween. The cooling jacket surrounds the venturi nozzle and a suction tube through which the fluid is being drawn into the venturi nozzle. Coolant flows through the cooling jacket. The cooling jacket dissipates heat generated by the venturi nozzle to prevent vapor lock.

  1. Turbine blade cooling

    DOEpatents

    Staub, F.W.; Willett, F.T.

    1999-07-20

    A turbine rotor blade comprises a shank portion, a tip portion and an airfoil. The airfoil has a pressure side wall and a suction side wall that are interconnected by a plurality of partition sidewalls, defining an internal cooling passageway within the airfoil. The internal cooling passageway includes at least one radial outflow passageway to direct a cooling medium flow from the shank portion towards the tip portion and at least one radial inflow passageway to direct a cooling medium flow from the tip portion towards the shank portion. A number of mixing ribs are disposed on the partition sidewalls within the radial outflow passageways so as to enhance the thermal mixing of the cooling medium flow, thereby producing improved heat transfer over a broad range of the Buoyancy number. 13 figs.

  2. Turbine blade cooling

    DOEpatents

    Staub, Fred Wolf; Willett, Fred Thomas

    2000-01-01

    A turbine rotor blade comprises a shank portion, a tip portion and an airfoil. The airfoil has a pressure side wall and a suction side wall that are interconnected by a plurality of partition sidewalls, defining an internal cooling passageway within the airfoil. The internal cooling passageway includes at least one radial outflow passageway to direct a cooling medium flow from the shank portion towards the tip portion and at least one radial inflow passageway to direct a cooling medium flow from the tip portion towards the shank portion. A number of mixing ribs are disposed on the partition sidewalls within the radial outflow passageways so as to enhance the thermal mixing of the cooling medium flow, thereby producing improved heat transfer over a broad range of the Buoyancy number.

  3. Turbine blade cooling

    DOEpatents

    Staub, Fred Wolf; Willett, Fred Thomas

    1999-07-20

    A turbine rotor blade comprises a shank portion, a tip portion and an airfoil. The airfoil has a pressure side wall and a suction side wall that are interconnected by a plurality of partition sidewalls, defining an internal cooling passageway within the airfoil. The internal cooling passageway includes at least one radial outflow passageway to direct a cooling medium flow from the shank portion towards the tip portion and at least one radial inflow passageway to direct a cooling medium flow from the tip portion towards the shank portion. A number of mixing ribs are disposed on the partition sidewalls within the radial outflow passageways so as to enhance the thermal mixing of the cooling medium flow, thereby producing improved heat transfer over a broad range of the Buoyancy number.

  4. Hydronic rooftop cooling systems

    DOEpatents

    Bourne, Richard C.; Lee, Brian Eric; Berman, Mark J.

    2008-01-29

    A roof top cooling unit has an evaporative cooling section that includes at least one evaporative module that pre-cools ventilation air and water; a condenser; a water reservoir and pump that captures and re-circulates water within the evaporative modules; a fan that exhausts air from the building and the evaporative modules and systems that refill and drain the water reservoir. The cooling unit also has a refrigerant section that includes a compressor, an expansion device, evaporator and condenser heat exchangers, and connecting refrigerant piping. Supply air components include a blower, an air filter, a cooling and/or heating coil to condition air for supply to the building, and optional dampers that, in designs that supply less than 100% outdoor air to the building, control the mixture of return and ventilation air.

  5. Stochastic cooling at Fermilab

    SciTech Connect

    Marriner, J.

    1986-08-01

    The topics discussed are the stochastic cooling systems in use at Fermilab and some of the techniques that have been employed to meet the particular requirements of the anti-proton source. Stochastic cooling at Fermilab became of paramount importance about 5 years ago when the anti-proton source group at Fermilab abandoned the electron cooling ring in favor of a high flux anti-proton source which relied solely on stochastic cooling to achieve the phase space densities necessary for colliding proton and anti-proton beams. The Fermilab systems have constituted a substantial advance in the techniques of cooling including: large pickup arrays operating at microwave frequencies, extensive use of cryogenic techniques to reduce thermal noise, super-conducting notch filters, and the development of tools for controlling and for accurately phasing the system.

  6. Water cooled steam jet

    DOEpatents

    Wagner, E.P. Jr.

    1999-01-12

    A water cooled steam jet for transferring fluid and preventing vapor lock, or vaporization of the fluid being transferred, has a venturi nozzle and a cooling jacket. The venturi nozzle produces a high velocity flow which creates a vacuum to draw fluid from a source of fluid. The venturi nozzle has a converging section connected to a source of steam, a diffuser section attached to an outlet and a throat portion disposed there between. The cooling jacket surrounds the venturi nozzle and a suction tube through which the fluid is being drawn into the venturi nozzle. Coolant flows through the cooling jacket. The cooling jacket dissipates heat generated by the venturi nozzle to prevent vapor lock. 2 figs.

  7. Late Pleistocene desiccation of Lake Tana, source of the Blue Nile

    NASA Astrophysics Data System (ADS)

    Lamb, Henry F.; Bates, C. Richard; Coombes, Paul V.; Marshall, Michael H.; Umer, Mohammed; Davies, Sarah J.; Dejen, Eshete

    2007-02-01

    High-resolution seismic data from Lake Tana, the source of the Blue Nile in northern Ethiopia, reveal a deep sedimentary sequence divided by four strong reflectors. Data from nearshore cores show that the uppermost strong reflector represents a stiff silt unit, interpreted as a desiccation surface. Channel cuts in this surface, bordered by levee-like structures, are apparent in the seismic data from near the lake margin, suggesting fluvial downcutting and over-bank deposition during seasonal flood events. Periphytic diatoms and peat at the base of a core from the deepest part of the lake overlie compacted sediments, indicating that desiccation was followed by development of shallow-water environments and papyrus swamp in the central basin between 16,700 and 15,100 cal BP. As the lake level rose, open-water evaporation from the closed lake caused it to become slightly saline, as indicated by halophytic diatoms. An abrupt return to freshwater conditions occurred at 14,750 cal BP, when the lake overflowed into the Blue Nile. Further reflection surfaces with downcut structures are identifiable in seismic images of the overlying sediments, suggesting at least two lesser lake-level falls, tentatively dated to about 12,000 and 8000 cal BP. Since Lake Victoria, the source of the White Nile, was also dry until 15,000 cal BP, and did not reach overflow until 14,500 cal BP, the entire Nile system must have been reduced to intermittent seasonal flow until about 14,500 cal BP, when baseflow was re-established with almost simultaneous overflow of the headwater lakes of both the White and Blue Nile rivers. Desiccation of the Nile sources coincides with Heinrich event 1, when cessation of northward heat transport from the tropical Atlantic disrupted the Atlantic monsoon, causing drought in north tropical Africa. The strong reflectors at deeper levels in the seismic sequence of Lake Tana may represent earlier desiccation events, possibly contemporaneous with previous Late

  8. Investigation of Desiccants and CO2 Sorbents for Advanced Exploration Systems 2015-2016

    NASA Technical Reports Server (NTRS)

    Knox, James C.; Watson, David W.; Wingard, Charles D.; West, Phillip W.; Cmarik, Gregory E.; Miller, Lee A.

    2016-01-01

    Advanced Exploration Systems are integral to crewed missions beyond low earth orbit and beyond the moon. The long-term goal is to reach Mars and return to Earth, but current air revitalization systems are not capable of extended operation within the mass, power, and volume requirements of such a mission. Two primary points are the mechanical stability of sorbent pellets and recovery of sorbent productivity after moisture exposure in the event of a leak. In this paper, we discuss the present efforts towards screening and characterizing commercially-available sorbents for extended operation in desiccant and CO2 removal beds.

  9. The influence of abdominal pigmentation on desiccation and ultraviolet resistance in two species of Drosophila.

    PubMed

    Matute, Daniel R; Harris, Alexandra

    2013-08-01

    Drosophila yakuba and D. santomea are sister species that differ in their levels of abdominal pigmentation; D. yakuba shows heavily pigmented posterior abdominal segments in both sexes, whereas D. santomea lacks dark pigment anywhere on its body. Using naturally collected lines, we demonstrate the existence of altitudinal variation in abdominal pigmentation in D. yakuba but not in D. santomea. We use the variation in pigmentation within D. yakuba and two body-color mutants in D. yakuba to elucidate selective advantage of differences in pigmentation. Our results indicate that although differences in abdominal pigmentation have no effect on desiccation resistance, lighter pigmentation confers ultraviolet radiation resistance in this pair of species.

  10. Subtask 5.10 - Testing of an Advanced Dry Cooling Technology for Power Plants

    SciTech Connect

    Martin, Christopher; Pavlish, John

    2013-09-30

    The University of North Dakota’s Energy & Environmental Research Center (EERC) is developing a market-focused dry cooling technology that is intended to address the key shortcomings of conventional dry cooling technologies: high capital cost and degraded cooling performance during daytime temperature peaks. The unique aspect of desiccant dry cooling (DDC) is the use of a hygroscopic working fluid—a liquid desiccant—as a heat-transfer medium between a power plant’s steam condenser and the atmosphere. This configuration enables a number of beneficial features for large-scale heat dissipation to the atmosphere, without the consumptive use of cooling water. The overall goal of this project was to accurately define the performance and cost characteristics of DDC to determine if further development of the concept is warranted. A balanced approach of modeling grounded in applied experimentation was pursued to substantiate DDC-modeling efforts and outline the potential for this technology to cool full-scale power plants. The resulting analysis shows that DDC can be a lower-cost dry cooling alternative to an air-cooled condenser (ACC) and can even be competitive with conventional wet recirculating cooling under certain circumstances. This project has also highlighted the key technological steps that must be taken in order to transfer DDC into the marketplace. To address these issues and to offer an extended demonstration of DDC technology, a next-stage project should include the opportunity for outdoor ambient testing of a small DDC cooling cell. This subtask was funded through the EERC–U.S. Department of Energy (DOE) Joint Program on Research and Development for Fossil Energy-Related Resources Cooperative Agreement No. DE-FC26-08NT43291. Nonfederal funding was provided by the Wyoming State Legislature under an award made through the Wyoming Clean Coal Technologies Research Program.

  11. Gas-fired desiccant dehumidification system field evaluation in a quick-service restaurant. Final report, October 1989

    SciTech Connect

    Koopman, R.N.; Marciniak, T.J.

    1989-10-01

    This report describes the results of a field evaluation of state-of-art desiccant dehumidification equipment in Houston, TX. The evaluation demonstrated that comfort control in a quick-service restaurant could be improved dramatically. However, available gas-fired desiccant dehumidification equipment is too expensive, inefficient, and unreliable to be considered for wide application in the restaurant industry. Results of a technical and economic analysis of four HVAC options in four U.S. cities indicated that improved comfort control could be achieved with only a modest increase in operating costs with an advanced system. This, coupled with the economic benefits achieved through lower indoor humidity such as improved crew performance and reduced maintenance costs, could justify the introduction of an advanced, integrated, HVAC system using desiccant technology which has an installed cost similar to current equipment.

  12. Solvent-Substitution Effects on Weight and Volume Changes during the Desiccation Process of Egg-White Gel

    NASA Astrophysics Data System (ADS)

    Kanaya, Haruichi; Hara, Kazuhiro; Takushi, Eisei; Matsushige, Kazumi

    1993-06-01

    The substitution effects for ethanol and acetone were investigated by means of simultaneous measurements of weight and volume during the desiccating process of egg-white gel. The time variations of the weight and volume in ethanol- and acetone-substituted gels were much different from those in the gel without substitution; the former showed gradual decrease, while the latter showed linear time dependence approximated by two lines in the semi-log plots. The structures in the desiccated specimens substituted with ethanol or acetone were revealed, through the scanning electron microscope (SEM) and X-ray photographs to differ greatly from those prepared from the nonsubstituted gel. Moreover, in the dehydration measurements carried out after soaking the desiccated specimens in water, all of the specimens showed almost the same features as the gel without substitution.

  13. Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativa L.).

    PubMed

    Siddique, Abu Baker; Ara, Israt; Islam, S M Shahinul; Tuteja, Narendra

    2014-01-01

    Enhancement of callus induction and its regeneration efficiency through in vitro techniques has been optimized for 2 abiotic stresses (salt and air desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI dhan47. The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL(-1)) and Kin (1.0 mgL(-1)). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7 gL(-1)) were used and its effect was recorded on the basis of viability of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content (RWC). It was observed that in all cases BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL(-1) NaCl. Plant regeneration capability was recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h. In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h. It was observed that air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls. Furthermore, desiccated calli also showed the better capability to survive in NaCl induced abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7 gL(-1) salt level for BRRI dhan47. Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.

  14. Nitrogen Limitation and Slow Drying Induce Desiccation Tolerance in Conjugating Green Algae (Zygnematophyceae, Streptophyta) from Polar Habitats

    PubMed Central

    Pichrtová, Martina; Kulichová, Jana; Holzinger, Andreas

    2014-01-01

    Background Filamentous Zygnematophyceae are typical components of algal mats in the polar hydro-terrestrial environment. Under field conditions, they form senescent vegetative cells, designated as pre-akinetes, which are tolerant to desiccation and osmotic stress. Key Findings Pre-akinete formation and desiccation tolerance was investigated experimentally under monitored laboratory conditions in four strains of Arctic and Antarctic isolates with vegetative Zygnema sp. morphology. Phylogenetic analyses of rbcL sequences revealed one Arctic strain as genus Zygnemopsis, phylogenetically distant from the closely related Zygnema strains. Algae were cultivated in liquid or on solidified medium (9 weeks), supplemented with or lacking nitrogen. Nitrogen-free cultures (liquid as well as solidified) consisted of well-developed pre-akinetes after this period. Desiccation experiments were performed at three different drying rates (rapid: 10% relative humidity, slow: 86% rh and very slow); viability, effective quantum yield of PS II, visual and ultrastructural changes were monitored. Recovery and viability of pre-akinetes were clearly dependent on the drying rate: slower desiccation led to higher levels of survival. Pre-akinetes survived rapid drying after acclimation by very slow desiccation. Conclusions The formation of pre-akinetes in polar Zygnema spp. and Zygnemopsis sp. is induced by nitrogen limitation. Pre-akinetes, modified vegetative cells, rather than specialized stages of the life cycle, can be hardened by mild desiccation stress to survive rapid drying. Naturally hardened pre-akinetes play a key role in stress tolerance and dispersal under the extreme conditions of polar regions, where sexual reproduction and production of dormant stages is largely suppressed. PMID:25398135

  15. Effect of desiccation on the dynamics of genome-wide DNA methylation in orthodox seeds of Acer platanoides L.

    PubMed

    Plitta, Beata P; Michalak, Marcin; Bujarska-Borkowska, Barbara; Barciszewska, Mirosława Z; Barciszewski, Jan; Chmielarz, Paweł

    2014-12-01

    5-methylcytosine, an abundant epigenetic mark, plays an important role in the regulation of plant growth and development, but there is little information about stress-induced changes in DNA methylation in seeds. In the present study, changes in a global level of m5C were measured in orthodox seeds of Acer platanoides L. during seed desiccation from a WC of 1.04 to 0.05-0.06 g H2O g g(-1) dry mass (g g(-1)). Changes in the level of DNA methylation were measured using 2D TLC e based method. Quality of desiccated seeds was examined by germination and seedling emergence tests. Global m5C content (R2)increase was observed in embryonic axes isolated from seeds collected at a high WC of 1.04 g g(-1) after their desiccation to significantly lower WC of 0.17 and 0.19 g g(-1). Further desiccation of these seeds to a WC of 0.06 g g(-1), however, resulted in a significant DNA demethylation to R2 ¼ 11.52-12.22%. Similar m5C decrease was observed in seeds which undergo maturation drying on the tree and had four times lower initial WC of 0.27 g g(-1) at the time of harvest, as they were dried to a WC of 0.05 g g(-1). These data confirm that desiccation induces changes in seed m5C levels. Results were validated by seed lots derived from tree different A. platanoides provenances. It is plausible that sine wave-like alterations in m5C amount may represent a specific response of orthodox seeds to drying and play a relevant role in desiccation tolerance in seeds.

  16. The desert moss Pterygoneurum lamellatum (Pottiaceae) exhibits an inducible ecological strategy of desiccation tolerance: effects of rate of drying on shoot damage and regeneration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Premise of the study: Bryophytes are regarded as a clade incorporating constitutive desiccation tolerance, especially terrestrial species. Here we test the hypothesis that the opposing ecological strategy of desiccation tolerance, inducibility, is present in a desert moss, and addressed by varying r...

  17. Evaluation of the desiccation tolerance of blastospores of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomyces)using a lab- scale, air-drying chamber with controlled relative humidity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The stabilization of living microbial agents for use as biological control agents is often accomplished through desiccation. The drying process must be conducive to the survival of the living microbial agent during desiccation and storage. Our air-drying studies with liquid culture-produced blasto...

  18. MEIC electron cooling program

    SciTech Connect

    Derbenev, Yaroslav S.; Zhang, Yuhong

    2014-12-01

    Cooling of proton and ion beams is essential for achieving high luminosities (up to above 1034 cm-2s-1) for MEIC, a Medium energy Electron-Ion Collider envisioned at JLab [1] for advanced nuclear science research. In the present conceptual design, we utilize the conventional election cooling method and adopted a multi-staged cooling scheme for reduction of and maintaining low beam emittances [2,3,4]. Two electron cooling facilities are required to support the scheme: one is a low energy (up to 2 MeV) DC cooler installed in the MEIC ion pre-booster (with the proton kinetic energy up to 3 GeV); the other is a high electron energy (up to 55 MeV) cooler in the collider ring (with the proton kinetic energy from 25 to 100 GeV). The high energy cooler, which is based on the ERL technology and a circulator ring, utilizes a bunched electron beam to cool bunched proton or ion beams. To complete the MEIC cooling concept and a technical design of the ERL cooler as well as to develop supporting technologies, an R&D program has been initiated at Jefferson Lab and significant progresses have been made since then. In this study, we present a brief description of the cooler design and a summary of the progress in this cooling R&D.

  19. MEIC electron cooling program

    DOE PAGESBeta

    Derbenev, Yaroslav S.; Zhang, Yuhong

    2014-12-01

    Cooling of proton and ion beams is essential for achieving high luminosities (up to above 1034 cm-2s-1) for MEIC, a Medium energy Electron-Ion Collider envisioned at JLab [1] for advanced nuclear science research. In the present conceptual design, we utilize the conventional election cooling method and adopted a multi-staged cooling scheme for reduction of and maintaining low beam emittances [2,3,4]. Two electron cooling facilities are required to support the scheme: one is a low energy (up to 2 MeV) DC cooler installed in the MEIC ion pre-booster (with the proton kinetic energy up to 3 GeV); the other is amore » high electron energy (up to 55 MeV) cooler in the collider ring (with the proton kinetic energy from 25 to 100 GeV). The high energy cooler, which is based on the ERL technology and a circulator ring, utilizes a bunched electron beam to cool bunched proton or ion beams. To complete the MEIC cooling concept and a technical design of the ERL cooler as well as to develop supporting technologies, an R&D program has been initiated at Jefferson Lab and significant progresses have been made since then. In this study, we present a brief description of the cooler design and a summary of the progress in this cooling R&D.« less

  20. Transcriptomics of Desiccation Tolerance in the Streptophyte Green Alga Klebsormidium Reveal a Land Plant-Like Defense Reaction

    PubMed Central

    Holzinger, Andreas; Kaplan, Franziska; Blaas, Kathrin; Zechmann, Bernd; Komsic-Buchmann, Karin; Becker, Burkhard

    2014-01-01

    Background Water loss has significant effects on physiological performance and survival rates of algae. However, despite the prominent presence of aeroterrestrial algae in terrestrial habitats, hardly anything is known about the molecular events that allow aeroterrestrial algae to survive harsh environmental conditions. We analyzed the transcriptome and physiology of a strain of the alpine aeroterrestrial alga Klebsormidium crenulatum under control and strong desiccation-stress conditions. Principal Findings For comparison we first established a reference transcriptome. The high-coverage reference transcriptome includes about 24,183 sequences (1.5 million reads, 636 million bases). The reference transcriptome encodes for all major pathways (energy, carbohydrates, lipids, amino acids, sugars), nearly all deduced pathways are complete or missing only a few transcripts. Upon strong desiccation, more than 7000 transcripts showed changes in their expression levels. Most of the highest up-regulated transcripts do not show similarity to known viridiplant proteins, suggesting the existence of some genus- or species-specific responses to desiccation. In addition, we observed the up-regulation of many transcripts involved in desiccation tolerance in plants (e.g. proteins similar to those that are abundant in late embryogenesis (LEA), or proteins involved in early response to desiccation ERD), and enzymes involved in the biosynthesis of the raffinose family of oligosaccharides (RFO) known to act as osmolytes). Major physiological shifts are the up-regulation of transcripts for photosynthesis, energy production, and reactive oxygen species (ROS) metabolism, which is supported by elevated cellular glutathione content as revealed by immunoelectron microscopy as well as an increase in total antiradical power. However, the effective quantum yield of Photosystem II and CO2 fixation decreased sharply under the applied desiccation stress. In contrast, transcripts for cell integrative

  1. Cryogenic generator cooling

    NASA Astrophysics Data System (ADS)

    Eckels, P. W.; Fagan, T. J.; Parker, J. H., Jr.; Long, L. J.; Shestak, E. J.; Calfo, R. M.; Hannon, W. F.; Brown, D. B.; Barkell, J. W.; Patterson, A.

    The concept for a hydrogen cooled aluminum cryogenic generator was presented by Schlicher and Oberly in 1985. Following their lead, this paper describes the thermal design of a high voltage dc, multimegawatt generator of high power density. The rotor and stator are cooled by saturated liquid and supercritical hydrogen, respectively. The brushless exciter on the same shaft is also cooled by liquid hydrogen. Component development testing is well under way and some of the test results concerning the thermohydraulic performance of the conductors are reported. The aluminum cryogenic generator's characteristics are attractive for hydrogen economy applications.

  2. The Effect of Sub-MIC β-Lactam Antibiotic Exposure of Pseudomonas aeruginosa Strains from People with Cystic Fibrosis in a Desiccation Survival Model.

    PubMed

    Clifton, I J; Denton, M; M'zali, F; Peckham, D G

    2011-01-01

    Prior to modern typing methods, cross-infection of P. aeruginosa between people with cystic fibrosis (CF) was felt to be rare. Recently a number of studies have demonstrated the presence of clonal strains of P. aeruginosa infecting people with CF. The aim of this study was to determine whether strains of P. aeruginosa demonstrated differences in resistance to desiccation and whether preincubation in subminimum inhibitory concentrations (MICs) of β-lactam affected desiccation resistance. The experimental data were modelled to a first-order decay model and a Weibull decay model using least squares nonlinear regression. The Weibull model was the preferred model for the desiccation survival. The presence of a mucoid phenotype promoted desiccation survival. Preincubation with antibiotics did not have a consistent effect on the strains of P. aeruginosa. Meropenem reduced desiccation resistance, whereas ceftazidime had much less effect on the strains studied.

  3. Desiccation Tolerance in the Tardigrade Richtersius coronifer Relies on Muscle Mediated Structural Reorganization

    PubMed Central

    Halberg, Kenneth Agerlin; Jørgensen, Aslak; Møbjerg, Nadja

    2013-01-01

    Life unfolds within a framework of constraining abiotic factors, yet some organisms are adapted to handle large fluctuations in physical and chemical parameters. Tardigrades are microscopic ecdysozoans well known for their ability to endure hostile conditions, such as complete desiccation – a phenomenon called anhydrobiosis. During dehydration, anhydrobiotic animals undergo a series of anatomical changes. Whether this reorganization is an essential regulated event mediated by active controlled processes, or merely a passive result of the dehydration process, has not been clearly determined. Here, we investigate parameters pivotal to the formation of the so-called "tun", a state that in tardigrades and rotifers marks the entrance into anhydrobiosis. Estimation of body volume in the eutardigrade Richtersius coronifer reveals an 87 % reduction in volume from the hydrated active state to the dehydrated tun state, underlining the structural stress associated with entering anhydrobiosis. Survival experiments with pharmacological inhibitors of mitochondrial energy production and muscle contractions show that i) mitochondrial energy production is a prerequisite for surviving desiccation, ii) uncoupling the mitochondria abolishes tun formation, and iii) inhibiting the musculature impairs the ability to form viable tuns. We moreover provide a comparative analysis of the structural changes involved in tun formation, using a combination of cytochemistry, confocal laser scanning microscopy and 3D reconstructions as well as scanning electron microscopy. Our data reveal that the musculature mediates a structural reorganization vital for anhydrobiotic survival, and furthermore that maintaining structural integrity is essential for resumption of life following rehydration. PMID:24391987

  4. Rapid Recovery of Cyanobacterial Pigments in Desiccated Biological Soil Crusts following Addition of Water

    PubMed Central

    Abed, Raeid M. M.; Polerecky, Lubos; Al-Habsi, Amal; Oetjen, Janina; Strous, Marc; de Beer, Dirk

    2014-01-01

    We examined soil surface colour change to green and hydrotaxis following addition of water to biological soil crusts using pigment extraction, hyperspectral imaging, microsensors and 13C labeling experiments coupled to matrix-assisted laser desorption and ionization time of flight-mass spectrometry (MALD-TOF MS). The topsoil colour turned green in less than 5 minutes following water addition. The concentrations of chlorophyll a (Chl a), scytonemin and echinenon rapidly increased in the top <1 mm layer while in the deeper layer, their concentrations remained low. Hyperspectral imaging showed that, in both wet and dehydrated crusts, cyanobacteria formed a layer at a depth of 0.2–0.4 mm and this layer did not move upward after wetting. 13C labeling experiments and MALDI TOF analysis showed that Chl a was already present in the desiccated crusts and de novo synthesis of this molecule started only after 2 days of wetting due to growth of cyanobacteria. Microsensor measurements showed that photosynthetic activity increased concomitantly with the increase of Chl a, and reached a maximum net rate of 92 µmol m−2 h−1 approximately 2 hours after wetting. We conclude that the colour change of soil crusts to green upon water addition was not due to hydrotaxis but rather to the quick recovery and reassembly of pigments. Cyanobacteria in crusts can maintain their photosynthetic apparatus intact even under prolonged periods of desiccation with the ability to resume their photosynthetic activities within minutes after wetting. PMID:25375172

  5. Need for desiccant in containers exposed to atmospheric conditions for long periods of time

    SciTech Connect

    Mead, K.E.

    1981-11-01

    Current component and system designs are required to perform satisfactorily up to 25 years. A maximum leak rate of 1 x 10/sup -6/ cc(STP) helium/sec-atm is a frequent requirement for component containers. Calculations show that undesiccated component containers continuously exposed to 50% relative humidity at 20/sup 0/C and having an internal free volume of less than 300 cc and the above leak rate will allow the internal dew point to rise enough for potential liquid condensation in less than four years. For the same vapor pressure differential, the moisture permeation rate through one linear inch of silicone o-ring is 750 times as fast as moisture enters a welded container whose leak rate is 1 x 10/sup -6/ cc(STP) helium/sec-atm. For ethylene propylene o-ring material this ratio is about 13. These values correspond to the ratios of the quantities of desiccant required to maintain an acceptable dew point temperature when the moisture capacity of the free volume is not included. Charts are provided for estimating the amount of desiccant required for helium leak tested containers and for containers sealed with elastomeric o-rings.

  6. Antarctic glacio-eustatic contributions to late Miocene Mediterranean desiccation and reflooding.

    PubMed

    Ohneiser, Christian; Florindo, Fabio; Stocchi, Paolo; Roberts, Andrew P; DeConto, Robert M; Pollard, David

    2015-11-10

    The Messinian Salinity Crisis (MSC) was a marked late Neogene oceanographic event during which the Mediterranean Sea evaporated. Its causes remain unresolved, with tectonic restrictions to the Atlantic Ocean or glacio-eustatic restriction of flow during sea-level lowstands, or a mixture of the two mechanisms, being proposed. Here we present the first direct geological evidence of Antarctic ice-sheet (AIS) expansion at the MSC onset and use a δ(18)O record to model relative sea-level changes. Antarctic sedimentary successions indicate AIS expansion at 6 Ma coincident with major MSC desiccation; relative sea-level modelling indicates a prolonged ∼50 m lowstand at the Strait of Gibraltar, which resulted from AIS expansion and local evaporation of sea water in concert with evaporite precipitation that caused lithospheric deformation. Our results reconcile MSC events and demonstrate that desiccation and refilling were timed by the interplay between glacio-eustatic sea-level variations, glacial isostatic adjustment and mantle deformation in response to changing water and evaporite loads.

  7. Endogenous Small-Noncoding RNAs and Potential Functions in Desiccation Tolerance in Physcomitrella Patens.

    PubMed

    Xia, Jing; Wang, Xiaoqin; Perroud, Pierre-François; He, Yikun; Quatrano, Ralph; Zhang, Weixiong

    2016-01-01

    Early land plants like moss Physcomitrella patens have developed remarkable drought tolerance. Phytohormone abscisic acid (ABA) protects seeds during water stress by activating genes through transcription factors such as ABSCISIC ACID INSENSITIVE (ABI3). Small noncoding RNA (sncRNA), including microRNAs (miRNAs) and endogenous small-interfering RNAs (endo-siRNAs), are key gene regulators in eukaryotes, playing critical roles in stress tolerance in plants. Combining next-generation sequencing and computational analysis, we profiled and characterized sncRNA species from two ABI3 deletion mutants and the wild type P. patens that were subject to ABA treatment in dehydration and rehydration stages. Small RNA profiling using deep sequencing helped identify 22 novel miRNAs and 6 genomic loci producing trans-acting siRNAs (ta-siRNAs) including TAS3a to TAS3e and TAS6. Data from degradome profiling showed that ABI3 genes (ABI3a/b/c) are potentially regulated by the plant-specific miR536 and that other ABA-relevant genes are regulated by miRNAs and ta-siRNAs. We also observed broad variations of miRNAs and ta-siRNAs expression across different stages, suggesting that they could potentially influence desiccation tolerance. This study provided evidence on the potential roles of sncRNA in mediating desiccation-responsive pathways in early land plants. PMID:27443635

  8. The re-establishment of desiccation tolerance in germinated Arabidopsis thaliana seeds and its associated transcriptome.

    PubMed

    Maia, Julio; Dekkers, Bas J W; Provart, Nicholas J; Ligterink, Wilco; Hilhorst, Henk W M

    2011-01-01

    The combination of robust physiological models with "omics" studies holds promise for the discovery of genes and pathways linked to how organisms deal with drying. Here we used a transcriptomics approach in combination with an in vivo physiological model of re-establishment of desiccation tolerance (DT) in Arabidopsis thaliana seeds. We show that the incubation of desiccation sensitive (DS) germinated Arabidopsis seeds in a polyethylene glycol (PEG) solution re-induces the mechanisms necessary for expression of DT. Based on a SNP-tile array gene expression profile, our data indicates that the re-establishment of DT, in this system, is related to a programmed reversion from a metabolic active to a quiescent state similar to prior to germination. Our findings show that transcripts of germinated seeds after the PEG-treatment are dominated by those encoding LEA, seed storage and dormancy related proteins. On the other hand, a massive repression of genes belonging to many other classes such as photosynthesis, cell wall modification and energy metabolism occurs in parallel. Furthermore, comparison with a similar system for Medicago truncatula reveals a significant overlap between the two transcriptomes. Such overlap may highlight core mechanisms and key regulators of the trait DT. Taking into account the availability of the many genetic and molecular resources for Arabidopsis, the described system may prove useful for unraveling DT in higher plants. PMID:22195004

  9. Sediment desiccation as a driver of phosphate availability in the water column of Mediterranean wetlands.

    PubMed

    Gilbert, Juan Diego; Guerrero, Francisco; de Vicente, Inmaculada

    2014-01-01

    Sediment desiccation is expected to drastically affect nutrient cycling in Mediterranean wetlands as global climate change models predict that many areas will become significantly drier than they currently are. In this study, we selected two Mediterranean wetlands that clearly differ in their water chemical composition (Honda and Hituelo wetlands) in order to determine the impact of sediment desiccation on phosphate (PO₄(3-)) adsorption and desorption properties. A decrease in PO₄(3-) sorption properties was observed in transects from the littoral zone to dry land in both lakes concomitantly with a reduction in organic matter content, revealing a critical role of organic matter for sequestering P in the lake sediment. Our experiments designed to determine if drying events would lead to an enhanced P release upon re-wetting have shown that, simulating natural conditions of re-flooding (that is without adding sodium azide), PO₄(3-) concentrations were notably higher in the overlying water than those initially measured in the lake water. These results highlight the impact of drying sediment and the subsequent re-wetting on increasing P concentrations in lake water and accordingly, affecting to lake trophic state. Finally, we aimed on determining the overall effect of biotic versus abiotic activity on P release patterns observed upon re-wetting. Our results have evidenced that while in Honda, biotic processes upon re-wetting are crucial for increasing P retention in the sediment; P exchange across sediment and water upon dry sediment re-wetting is basically mediated by abiotic processes in Hituelo.

  10. Dynamic performance of packed-bed dehumidifiers: experimental results from the SERI desiccant test loop

    SciTech Connect

    Kutscher, C F; Barlow, R S

    1982-08-01

    Discussed are the design and construction of a desiccant test loop and results of tests with a silica-gel-packed bed. The test loop consists of two centrifugal fans, two duct heaters, a steam humidifier, 24.4m (80 ft) of 30-cm (12-in.) circular duct, instrumentation, and a test section. The loop is capable of testing adsorption and desorption modes at flow rates up to 0.340 kg/s (600 scfm) and at regeneration temperatures up to 120/sup 0/C (248/sup 0/F). Tests of a 74-cm(29-in.)-diameter, 3.2-cm(1.25-in.)-thick silica gel bed indicated that mass transfer occurs more readily in the adsorption direction than in the desorption direction. Pressure drop data indicated that the resistance of each of the two screens that hold the silica gel in place was equivalent to 2.5-cm(1-in.) of silica gel due to plugging. Results of the tests were also used to validate a SERI desiccant computer model, DESSIM.

  11. Desiccation tolerance in the tardigrade Richtersius coronifer relies on muscle mediated structural reorganization.

    PubMed

    Halberg, Kenneth Agerlin; Jørgensen, Aslak; Møbjerg, Nadja

    2013-01-01

    Life unfolds within a framework of constraining abiotic factors, yet some organisms are adapted to handle large fluctuations in physical and chemical parameters. Tardigrades are microscopic ecdysozoans well known for their ability to endure hostile conditions, such as complete desiccation--a phenomenon called anhydrobiosis. During dehydration, anhydrobiotic animals undergo a series of anatomical changes. Whether this reorganization is an essential regulated event mediated by active controlled processes, or merely a passive result of the dehydration process, has not been clearly determined. Here, we investigate parameters pivotal to the formation of the so-called "tun", a state that in tardigrades and rotifers marks the entrance into anhydrobiosis. Estimation of body volume in the eutardigrade Richtersius coronifer reveals an 87 % reduction in volume from the hydrated active state to the dehydrated tun state, underlining the structural stress associated with entering anhydrobiosis. Survival experiments with pharmacological inhibitors of mitochondrial energy production and muscle contractions show that i) mitochondrial energy production is a prerequisite for surviving desiccation, ii) uncoupling the mitochondria abolishes tun formation, and iii) inhibiting the musculature impairs the ability to form viable tuns. We moreover provide a comparative analysis of the structural changes involved in tun formation, using a combination of cytochemistry, confocal laser scanning microscopy and 3D reconstructions as well as scanning electron microscopy. Our data reveal that the musculature mediates a structural reorganization vital for anhydrobiotic survival, and furthermore that maintaining structural integrity is essential for resumption of life following rehydration.

  12. Desiccation induces viable but Non-Culturable cells in Sinorhizobium meliloti 1021

    PubMed Central

    2012-01-01

    Sinorhizobium meliloti is a microorganism commercially used in the production of e.g. Medicago sativa seed inocula. Many inocula are powder-based and production includes a drying step. Although S. meliloti survives drying well, the quality of the inocula is reduced during this process. In this study we determined survival during desiccation of the commercial strains 102F84 and 102F85 as well as the model strain USDA1021. The survival of S. meliloti 1021 was estimated during nine weeks at 22% relative humidity. We found that after an initial rapid decline of colony forming units, the decline slowed to a steady 10-fold reduction in colony forming units every 22 days. In spite of the reduction in colony forming units, the fraction of the population identified as viable (42-54%) based on the Baclight live/dead stain did not change significantly over time. This change in the ability of viable cells to form colonies shows (i) an underestimation of the survival of rhizobial cells using plating methods, and that (ii) in a part of the population desiccation induces a Viable But Non Culturable (VBNC)-like state, which has not been reported before. Resuscitation attempts did not lead to a higher recovery of colony forming units indicating the VBNC state is stable under the conditions tested. This observation has important consequences for the use of rhizobia. Finding methods to resuscitate this fraction may increase the quality of powder-based seed inocula. PMID:22260437

  13. Ground penetrating radar and direct current resistivity evaluation of the desiccation test cap, Savannah River Site

    SciTech Connect

    Wyatt, D.E.; Cumbest, R.J.

    1996-04-01

    The Savannah River Site (SRS) has a variety of waste units that may be temporarily or permanently stabilized by closure using an impermeable cover to prevent groundwater infiltration. The placement of an engineered kaolin clay layer over a waste unit is an accepted and economical technique for providing an impermeable cover but the long term stability and integrity of the clay in non-arid conditions is unknown. A simulated kaolin cap has been constructed at the SRA adjacent to the Burial Ground Complex. The cap is designed to evaluate the effects of desiccation on clay integrity, therefore half of the cap is covered with native soil to prevent drying, while the remainder of the cap is exposed. Measurements of the continuing impermeability of a clay cap are difficult because intrusive techniques may locally compromise the structure. Point measurements made to evaluate clay integrity, such as those from grid sampling or coring and made through a soil cover, may miss cracks, joints or fissures, and may not allow for mapping of the lateral extent of elongate features. Because of these problems, a non-invasive technique is needed to map clay integrity, below a soil or vegetation cover, which is capable of moderate to rapid investigation speeds. Two non-intrusive geophysical techniques, direct current resistivity and ground penetrating radar (GPR), have been successful at the SRS in geologically mapping shallow subsurface clay layers. The applicability of each technique in detecting the clay layer in the desiccation test cap and associated anomalies was investigated.

  14. Antarctic glacio-eustatic contributions to late Miocene Mediterranean desiccation and reflooding

    PubMed Central

    Ohneiser, Christian; Florindo, Fabio; Stocchi, Paolo; Roberts, Andrew P.; DeConto, Robert M.; Pollard, David

    2015-01-01

    The Messinian Salinity Crisis (MSC) was a marked late Neogene oceanographic event during which the Mediterranean Sea evaporated. Its causes remain unresolved, with tectonic restrictions to the Atlantic Ocean or glacio-eustatic restriction of flow during sea-level lowstands, or a mixture of the two mechanisms, being proposed. Here we present the first direct geological evidence of Antarctic ice-sheet (AIS) expansion at the MSC onset and use a δ18O record to model relative sea-level changes. Antarctic sedimentary successions indicate AIS expansion at 6 Ma coincident with major MSC desiccation; relative sea-level modelling indicates a prolonged ∼50 m lowstand at the Strait of Gibraltar, which resulted from AIS expansion and local evaporation of sea water in concert with evaporite precipitation that caused lithospheric deformation. Our results reconcile MSC events and demonstrate that desiccation and refilling were timed by the interplay between glacio-eustatic sea-level variations, glacial isostatic adjustment and mantle deformation in response to changing water and evaporite loads. PMID:26556503

  15. Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds.

    PubMed

    Soares, Giuliana C M; Dias, Denise C F S; Faria, José M R; Borges, Eduardo E L

    2015-01-01

    We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds.

  16. ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging.

    PubMed

    Parkhey, Suruchi; Naithani, S C; Keshavkant, S

    2012-08-01

    Reactive oxygen species (ROS) and lipid peroxidation products appear to correlate strongly with the desiccation induced loss of viability in recalcitrant sal seeds. The 100% germination in fresh sal seeds declined with dehydration under natural storage conditions (26 ± 1 °C, relative humidity 52 ± 2%). Seeds became non-viable within 8 days. Desiccation induced disturbances in the metabolic activity of seeds resulted in generation of enormous amounts of ROS that are responsible for cellular damage and viability loss. Oxidative stress in the dehydrating aging sal seeds was further aggravated by inducing lipid peroxidation as the amounts of free fatty acid, conjugated diene, lipid hydroperoxide and secondary free radicals; malondialdehyde and 4-hydroxy-2-nonenal, were also promoted. In addition, significant rise in lipid degrading enzymes; lipase (EC 3.1.1.3) and lipoxygenase (LOX, EC 1.13.11.12) were detected in dehydrating sal seeds. Our results indicated multiple pathways (ROS, lipid peroxidation & lipase and LOX) that operate in the dehydrating recalcitrant sal seeds finally contributing to loss of viability.

  17. Bacterial survival in response to desiccation and high humidity at above zero and subzero temperatures

    NASA Astrophysics Data System (ADS)

    Yang, Yinjie; Yokobori, Shin-ichi; Yamagishi, Akihiko

    2009-04-01

    Earthly microorganisms might have contaminated Mars for millions of years by intellectual activities or natural transfer. Knowledge on the preservation of microorganisms may help our searching for life on outer planets, particularly Mars-contaminated earthly microorganisms at ancient time. Extreme dryness is one of the current Mars characteristics. However, a humid or watery Mars at earlier time was suggested by evidence accumulated in recent decades. It raises the question that whether water helps preservation of the microorganisms or not, particularly those with high possibility of interplanetary transfer like spores and Deinococci. In this study, we examined the effects of desiccation and high humidity on survival and DNA double strand breaks (DSB) of Escherichia coli, Deinococcus radiodurans and spores of Bacillus pumilus at 25, 4 and -70 °C. They exhibited different survival rates and DSB patterns under desiccation and high humidity. Higher survival and less DSB occurred at lower temperature. We suggest that some Mars-contaminated bacteria might have been viably preserved on cold Mars regions for long periods, regardless of water availability. It is more likely to find ancient spores than ancient Deinococci on Mars. In our search for preserved extraterrestrial life, priority should be given to the Mars Polar Regions.

  18. Combined effects of copper, desiccation, and frost on the viability of earthworm cocoons

    SciTech Connect

    Holmstrup, M.; Petersen, B.F. |; Larsen, M.M.

    1998-01-01

    The effects of heavy metal pollution on earthworms have been extensively studied, but no studies have examined how earthworms react if they are simultaneously exposed to metal pollution and climatic stress. This question has been addressed in a laboratory study where cocoons of Aporrectodea caliginosa and Dendrobaena octaedra were initially exposed to copper in aqueous solutions of copper chloride and thereafter exposed to realistic degrees of either desiccation or frost. Earthworm embryos absorbed copper in amounts comparable to concentrations found in various tissues of earthworms from metal-polluted soils. Desiccation and copper exposure in combination had synergistic effects on survival rates for both species. For example, at full saturation, the NOEC (the highest tested concentration with no statistically significant effect) for copper of A. caliginosa was 12 mg/L, whereas at 97% relative humidity it was only 6 mg/L. Frost and copper exposure in combination also showed synergistic effects in some experiments. No cocoons of A. caliginosa exposed to 20 mg copper/L were viable after exposure to {minus}3 C but at 0 C viability was as high as 95%. The same tendency was seen in D. octaedra but not as clearly as in A/. caliginosa. A change of the environmental conditions (moisture, temperature) to increasing severity caused a shift in the statistically derived NOEC toward lower critical values of copper. The involvement of combination effects in ecotoxicological tests could therefore improve risk assessment of soil-polluting compounds.

  19. Antarctic glacio-eustatic contributions to late Miocene Mediterranean desiccation and reflooding.

    PubMed

    Ohneiser, Christian; Florindo, Fabio; Stocchi, Paolo; Roberts, Andrew P; DeConto, Robert M; Pollard, David

    2015-01-01

    The Messinian Salinity Crisis (MSC) was a marked late Neogene oceanographic event during which the Mediterranean Sea evaporated. Its causes remain unresolved, with tectonic restrictions to the Atlantic Ocean or glacio-eustatic restriction of flow during sea-level lowstands, or a mixture of the two mechanisms, being proposed. Here we present the first direct geological evidence of Antarctic ice-sheet (AIS) expansion at the MSC onset and use a δ(18)O record to model relative sea-level changes. Antarctic sedimentary successions indicate AIS expansion at 6 Ma coincident with major MSC desiccation; relative sea-level modelling indicates a prolonged ∼50 m lowstand at the Strait of Gibraltar, which resulted from AIS expansion and local evaporation of sea water in concert with evaporite precipitation that caused lithospheric deformation. Our results reconcile MSC events and demonstrate that desiccation and refilling were timed by the interplay between glacio-eustatic sea-level variations, glacial isostatic adjustment and mantle deformation in response to changing water and evaporite loads. PMID:26556503

  20. Desiccation tolerance in the tardigrade Richtersius coronifer relies on muscle mediated structural reorganization.

    PubMed

    Halberg, Kenneth Agerlin; Jørgensen, Aslak; Møbjerg, Nadja

    2013-01-01

    Life unfolds within a framework of constraining abiotic factors, yet some organisms are adapted to handle large fluctuations in physical and chemical parameters. Tardigrades are microscopic ecdysozoans well known for their ability to endure hostile conditions, such as complete desiccation--a phenomenon called anhydrobiosis. During dehydration, anhydrobiotic animals undergo a series of anatomical changes. Whether this reorganization is an essential regulated event mediated by active controlled processes, or merely a passive result of the dehydration process, has not been clearly determined. Here, we investigate parameters pivotal to the formation of the so-called "tun", a state that in tardigrades and rotifers marks the entrance into anhydrobiosis. Estimation of body volume in the eutardigrade Richtersius coronifer reveals an 87 % reduction in volume from the hydrated active state to the dehydrated tun state, underlining the structural stress associated with entering anhydrobiosis. Survival experiments with pharmacological inhibitors of mitochondrial energy production and muscle contractions show that i) mitochondrial energy production is a prerequisite for surviving desiccation, ii) uncoupling the mitochondria abolishes tun formation, and iii) inhibiting the musculature impairs the ability to form viable tuns. We moreover provide a comparative analysis of the structural changes involved in tun formation, using a combination of cytochemistry, confocal laser scanning microscopy and 3D reconstructions as well as scanning electron microscopy. Our data reveal that the musculature mediates a structural reorganization vital for anhydrobiotic survival, and furthermore that maintaining structural integrity is essential for resumption of life following rehydration. PMID:24391987

  1. Endogenous Small-Noncoding RNAs and Potential Functions in Desiccation Tolerance in Physcomitrella Patens

    PubMed Central

    Xia, Jing; Wang, Xiaoqin; Perroud, Pierre-François; He, Yikun; Quatrano, Ralph; Zhang, Weixiong

    2016-01-01

    Early land plants like moss Physcomitrella patens have developed remarkable drought tolerance. Phytohormone abscisic acid (ABA) protects seeds during water stress by activating genes through transcription factors such as ABSCISIC ACID INSENSITIVE (ABI3). Small noncoding RNA (sncRNA), including microRNAs (miRNAs) and endogenous small-interfering RNAs (endo-siRNAs), are key gene regulators in eukaryotes, playing critical roles in stress tolerance in plants. Combining next-generation sequencing and computational analysis, we profiled and characterized sncRNA species from two ABI3 deletion mutants and the wild type P. patens that were subject to ABA treatment in dehydration and rehydration stages. Small RNA profiling using deep sequencing helped identify 22 novel miRNAs and 6 genomic loci producing trans-acting siRNAs (ta-siRNAs) including TAS3a to TAS3e and TAS6. Data from degradome profiling showed that ABI3 genes (ABI3a/b/c) are potentially regulated by the plant-specific miR536 and that other ABA-relevant genes are regulated by miRNAs and ta-siRNAs. We also observed broad variations of miRNAs and ta-siRNAs expression across different stages, suggesting that they could potentially influence desiccation tolerance. This study provided evidence on the potential roles of sncRNA in mediating desiccation-responsive pathways in early land plants. PMID:27443635

  2. Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds.

    PubMed

    Soares, Giuliana C M; Dias, Denise C F S; Faria, José M R; Borges, Eduardo E L

    2015-01-01

    We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds. PMID:26628022

  3. Key genes involved in desiccation tolerance and dormancy across life forms.

    PubMed

    Costa, Maria Cecília D; Farrant, Jill M; Oliver, Melvin J; Ligterink, Wilco; Buitink, Julia; Hilhorst, Henk M W

    2016-10-01

    Desiccation tolerance (DT, the ability of certain organisms to survive severe dehydration) was a key trait in the evolution of life in terrestrial environments. Likely, the development of desiccation-tolerant life forms was accompanied by the acquisition of dormancy or a dormancy-like stage as a second powerful adaptation to cope with variations in the terrestrial environment. These naturally stress tolerant life forms may be a good source of genetic information to generate stress tolerant crops to face a future with predicted higher occurrence of drought. By mining for key genes and mechanisms related to DT and dormancy conserved across different species and life forms, unique candidate key genes may be identified. Here we identify several of these putative key genes, shared among multiple organisms, encoding for proteins involved in protection, growth and energy metabolism. Mutating a selection of these genes in the model plant Arabidopsis thaliana resulted in clear DT-, dormancy- and other seed-associated phenotypes, showing the efficiency and power of our approach and paves the way for the development of drought-stress tolerant crops. Our analysis supports a co-evolution of DT and dormancy by shared mechanisms that favour survival and adaptation to ever-changing environments with strong seasonal fluctuations. PMID:27593474

  4. Evaporative Cooling Membrane Device

    NASA Technical Reports Server (NTRS)

    Lomax, Curtis (Inventor); Moskito, John (Inventor)

    1999-01-01

    An evaporative cooling membrane device is disclosed having a flat or pleated plate housing with an enclosed bottom and an exposed top that is covered with at least one sheet of hydrophobic porous material having a thin thickness so as to serve as a membrane. The hydrophobic porous material has pores with predetermined dimensions so as to resist any fluid in its liquid state from passing therethrough but to allow passage of the fluid in its vapor state, thereby, causing the evaporation of the fluid and the cooling of the remaining fluid. The fluid has a predetermined flow rate. The evaporative cooling membrane device has a channel which is sized in cooperation with the predetermined flow rate of the fluid so as to produce laminar flow therein. The evaporative cooling membrane device provides for the convenient control of the evaporation rates of the circulating fluid by adjusting the flow rates of the laminar flowing fluid.

  5. Warm and Cool Dinosaurs.

    ERIC Educational Resources Information Center

    Mannlein, Sally

    2001-01-01

    Presents an art activity in which first grade students draw dinosaurs in order to learn about the concept of warm and cool colors. Explains how the activity also helped the students learn about the concept of distance when drawing. (CMK)

  6. Stimulated radiative laser cooling

    NASA Astrophysics Data System (ADS)

    Muys, P.

    2008-04-01

    Building a refrigerator based on the conversion of heat into optical energy is an ongoing engineering challenge. Under well-defined conditions, spontaneous anti-Stokes fluorescence of a dopant material in a host matrix is capable of lowering the host temperature. The fluorescence is conveying away a part of the thermal energy stored in the vibrational oscillations of the host lattice. In particular, applying this principle to the cooling of (solid-state) lasers opens up many potential device applications, especially in the domain of high-power lasers. In this paper, an alternative optical cooling scheme is outlined, leading to the radiative cooling of solid-state lasers. It is based on converting the thermal energy stored in the host into optical energy by means of a stimulated nonlinear process, rather than a spontaneous process. This should lead to better cooling efficiencies and a higher potential of applying the principle for device applications.

  7. Why Exercise Is Cool

    MedlinePlus

    ... Homework? Here's Help White House Lunch Recipes Why Exercise Is Cool KidsHealth > For Kids > Why Exercise Is ... day and your body will thank you later! Exercise Makes Your Heart Happy You may know that ...

  8. Laser cooling of solids

    SciTech Connect

    Epstein, Richard I; Sheik-bahae, Mansoor

    2008-01-01

    We present an overview of solid-state optical refrigeration also known as laser cooling in solids by fluorescence upconversion. The idea of cooling a solid-state optical material by simply shining a laser beam onto it may sound counter intuitive but is rapidly becoming a promising technology for future cryocooler. We chart the evolution of this science in rare-earth doped solids and semiconductors.

  9. Refrigerant directly cooled capacitors

    DOEpatents

    Hsu, John S.; Seiber, Larry E.; Marlino, Laura D.; Ayers, Curtis W.

    2007-09-11

    The invention is a direct contact refrigerant cooling system using a refrigerant floating loop having a refrigerant and refrigeration devices. The cooling system has at least one hermetic container disposed in the refrigerant floating loop. The hermetic container has at least one electronic component selected from the group consisting of capacitors, power electronic switches and gating signal module. The refrigerant is in direct contact with the electronic component.

  10. WATER COOLED RETORT COVER

    DOEpatents

    Ash, W.J.; Pozzi, J.F.

    1962-05-01

    A retort cover is designed for use in the production of magnesium metal by the condensation of vaporized metal on a collecting surface. The cover includes a condensing surface, insulating means adjacent to the condensing surface, ind a water-cooled means for the insulating means. The irrangement of insulation and the cooling means permits the magnesium to be condensed at a high temperature and in massive nonpyrophoric form. (AEC)

  11. Weld electrode cooling study

    NASA Astrophysics Data System (ADS)

    Masters, Robert C.; Simon, Daniel L.

    1999-03-01

    The U.S. auto/truck industry has been mandated by the Federal government to continuously improve their fleet average gas mileage, measured in miles per gallon. Several techniques are typically used to meet these mandates, one of which is to reduce the overall mass of cars and trucks. To help accomplish this goal, lighter weight sheet metal parts, with smaller weld flanges, have been designed and fabricated. This paper will examine the cooling characteristics of various water cooled weld electrodes and shanks used in resistance spot welding applications. The smaller weld flanges utilized in modern vehicle sheet metal fabrications have increased industry's interest in using one size of weld electrode (1/2 inch diameter) for certain spot welding operations. The welding community wants more data about the cooling characteristics of these 1/2 inch weld electrodes. To hep define the cooling characteristics, an infrared radiometer thermal vision system (TVS) was used to capture images (thermograms) of the heating and cooling cycles of several size combinations of weld electrodes under typical production conditions. Tests results will show why the open ended shanks are more suitable for cooling the weld electrode assembly then closed ended shanks.

  12. Comparing Social Stories™ to Cool versus Not Cool

    ERIC Educational Resources Information Center

    Leaf, Justin B.; Mitchell, Erin; Townley-Cochran, Donna; McEachin, John; Taubman, Mitchell; Leaf, Ronald

    2016-01-01

    In this study we compared the cool versus not cool procedure to Social Stories™ for teaching various social behaviors to one individual diagnosed with autism spectrum disorder. The researchers randomly assigned three social skills to the cool versus not cool procedure and three social skills to the Social Stories™ procedure. Naturalistic probes…

  13. Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens.

    PubMed

    Yotsui, Izumi; Serada, Satoshi; Naka, Tetsuji; Saruhashi, Masashi; Taji, Teruaki; Hayashi, Takahisa; Quatrano, Ralph S; Sakata, Yoichi

    2016-03-18

    Desiccation tolerance is an ancestral feature of land plants and is still retained in non-vascular plants such as bryophytes and some vascular plants. However, except for seeds and spores, this trait is absent in vegetative tissues of vascular plants. Although many studies have focused on understanding the molecular basis underlying desiccation tolerance using transcriptome and proteome approaches, the critical molecular differences between desiccation tolerant plants and non-desiccation plants are still not clear. The moss Physcomitrella patens cannot survive rapid desiccation under laboratory conditions, but if cells of the protonemata are treated by the phytohormone abscisic acid (ABA) prior to desiccation, it can survive 24 h exposure to desiccation and regrow after rehydration. The desiccation tolerance induced by ABA (AiDT) is specific to this hormone, but also depends on a plant transcription factor ABSCISIC ACID INSENSITIVE3 (ABI3). Here we report the comparative proteomic analysis of AiDT between wild type and ABI3 deleted mutant (Δabi3) of P. patens using iTRAQ (Isobaric Tags for Relative and Absolute Quantification). From a total of 1980 unique proteins that we identified, only 16 proteins are significantly altered in Δabi3 compared to wild type after desiccation following ABA treatment. Among this group, three of the four proteins that were severely affected in Δabi3 tissue were Arabidopsis orthologous genes, which were expressed in maturing seeds under the regulation of ABI3. These included a Group 1 late embryogenesis abundant (LEA) protein, a short-chain dehydrogenase, and a desiccation-related protein. Our results suggest that at least three of these proteins expressed in desiccation tolerant cells of both Arabidopsis and the moss are very likely to play important roles in acquisition of desiccation tolerance in land plants. Furthermore, our results suggest that the regulatory machinery of ABA- and ABI3-mediated gene expression for desiccation

  14. Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens.

    PubMed

    Yotsui, Izumi; Serada, Satoshi; Naka, Tetsuji; Saruhashi, Masashi; Taji, Teruaki; Hayashi, Takahisa; Quatrano, Ralph S; Sakata, Yoichi

    2016-03-18

    Desiccation tolerance is an ancestral feature of land plants and is still retained in non-vascular plants such as bryophytes and some vascular plants. However, except for seeds and spores, this trait is absent in vegetative tissues of vascular plants. Although many studies have focused on understanding the molecular basis underlying desiccation tolerance using transcriptome and proteome approaches, the critical molecular differences between desiccation tolerant plants and non-desiccation plants are still not clear. The moss Physcomitrella patens cannot survive rapid desiccation under laboratory conditions, but if cells of the protonemata are treated by the phytohormone abscisic acid (ABA) prior to desiccation, it can survive 24 h exposure to desiccation and regrow after rehydration. The desiccation tolerance induced by ABA (AiDT) is specific to this hormone, but also depends on a plant transcription factor ABSCISIC ACID INSENSITIVE3 (ABI3). Here we report the comparative proteomic analysis of AiDT between wild type and ABI3 deleted mutant (Δabi3) of P. patens using iTRAQ (Isobaric Tags for Relative and Absolute Quantification). From a total of 1980 unique proteins that we identified, only 16 proteins are significantly altered in Δabi3 compared to wild type after desiccation following ABA treatment. Among this group, three of the four proteins that were severely affected in Δabi3 tissue were Arabidopsis orthologous genes, which were expressed in maturing seeds under the regulation of ABI3. These included a Group 1 late embryogenesis abundant (LEA) protein, a short-chain dehydrogenase, and a desiccation-related protein. Our results suggest that at least three of these proteins expressed in desiccation tolerant cells of both Arabidopsis and the moss are very likely to play important roles in acquisition of desiccation tolerance in land plants. Furthermore, our results suggest that the regulatory machinery of ABA- and ABI3-mediated gene expression for desiccation

  15. Osmotic stress and recovery in field populations of Zygnema sp. (Zygnematophyceae, Streptophyta) on Svalbard (High Arctic) subjected to natural desiccation.

    PubMed

    Pichrtová, Martina; Hájek, Tomáš; Elster, Josef

    2014-08-01

    Zygnema is a genus of filamentous green algae belonging to the class of Zygnematophyceae (Streptophyta). In the Arctic, it typically forms extensive mats in habitats that regularly dry out during summer, and therefore, mechanisms of stress resistance are expected. We investigated its natural populations with respect to production of specialized desiccation-resistant cells and osmotic acclimation. Six populations in various stages of natural desiccation were selected, from wet biomass floating in water to dried paper-like crusts. After rewetting, plasmolysis and osmotic stress effects were studied using hypertonic sorbitol solutions, and the physiological state was estimated using chlorophyll a fluorescence parameters. All populations of Zygnema sp. formed stationary-phase cells filled with storage products. In green algal research, such cells are traditionally called akinetes. However, the populations differed in their reaction to osmotic stress. Whereas the wet-collected samples were strongly impaired, the osmotic stress resistance of the naturally dried samples was comparable to that of true aeroterrestrial algae. We showed that arctic populations of Zygnema acclimate well to natural desiccation via hardening that is mediated by slow desiccation. As no other types of specialized cells were observed, we assume that the naturally hardened akinetes also play a key role in winter survival.

  16. Downregulation of dTps1 in Drosophila melanogaster larvae confirms involvement of trehalose in redox regulation following desiccation.

    PubMed

    Thorat, Leena; Mani, Krishna-Priya; Thangaraj, Pradeep; Chatterjee, Suvro; Nath, Bimalendu B

    2016-03-01

    As a survival strategy to environmental water deficits, desiccation-tolerant organisms are commonly known for their ability to recruit stress-protective biomolecules such as trehalose. We have previously reported the pivotal role of trehalose in larval desiccation tolerance in Drosophila melanogaster. Trehalose has emerged as a versatile molecule, serving mainly as energy source in insects and also being a stress protectant. While several recent reports have revealed the unconventional role of trehalose in scavenging reactive oxygen species in yeast and plants, this aspect has not received much attention in animals. We examined the status of desiccation-induced generation of reactive oxygen species in D. melanogaster larvae and the possible involvement of trehalose in ameliorating the harmful consequences thereof. Insect trehalose synthesis is governed by the enzyme trehalose 6-phosphate synthase 1 (TPS1). Using the ubiquitous da-GAL4-driven expression of the dTps1-RNAi transgene, we generated dTps1-downregulated Drosophila larvae possessing depleted levels of dTps1 transcripts. This resulted in the inability of the larvae for trehalose synthesis, thereby allowing us to elucidate the significance of trehalose in the regulation of desiccation-responsive redox homeostasis. Furthermore, the results from molecular genetics studies, biochemical assays, electron spin resonance analyses and a simple, non-invasive method of whole larval live imaging suggested that trehalose in collaboration with superoxide dismutase (SOD) is involved in the maintenance of redox state in D. melanogaster.

  17. Sealed tube comparisons of the compatibility of desiccants with refrigerants and lubricants. Final report, August 1993--January 1995

    SciTech Connect

    Field, J.E.

    1995-05-01

    Continuing environmental concerns mandate replacement of CFC`s with alternate refrigeration fluids. Until now, relatively little testing had been reported in the literature for compatibility of desiccants in these new working fluids. Using bench scale test methods generally accepted throughout the industry today, this work provides data necessary to assess the compatibility of virtually all of the currently used desiccant types - both bead and molded core, with thirteen refrigerant/lubricant combinations. The desiccants have been tested by exposure to refrigerant and lubricant in sealed, glass tubes in accordance with ASHRAE/ANSI Standard 97-1989. After aging, the liquid phase was evaluated for acid anion formation, change in color, and presence of halide ions, the gas phase was analyzed for refrigerant decomposition by gas chromatography, and the desiccants were evaluated for changes in crush strength and for retention of acids and halide ions. Metal catalysts, also present in the sealed tubes, were visually examined for corrosion, copper plating, and appearance changes.

  18. An oxidative burst of superoxide in embryonic axes of recalcitrant sweet chestnut seeds as induced by excision and desiccation.

    PubMed

    Roach, Thomas; Ivanova, Mariyana; Beckett, Richard P; Minibayeva, Farida V; Green, Ian; Pritchard, Hugh W; Kranner, Ilse

    2008-06-01

    Recalcitrant seeds are intolerant of desiccation and cannot be stored in conventional seed banks. Cryopreservation allows storage of the germplasm of some recalcitrant seeded species, but application to a wide range of plant diversity is still limited. The present work aimed at understanding the stresses that accompany the first steps in cryopreservation protocols, wounding and desiccation, both of which are likely to lead to the formation of reactive oxygen species (ROS). Extracellular ROS production was studied in isolated embryonic axes of sweet chestnut (Castanea sativa). Axis excision was accompanied by a burst of superoxide (O(2)(*-)), demonstrated by a colorimetric assay using epinephrine, electron spin resonance and staining with nitroblue tetrazolium. Superoxide was immediately produced on the cut surface after isolation of the axis from the seed, with an initial 'burst' in the first 5 min. Isolated axes subjected to variable levels of desiccation stress showed a decrease in viability and vigour and increased electrolyte leakage, indicative of impaired membrane integrity. The pattern of O(2)(*-) production showed a typical Gaussian pattern in response to increasing desiccation stress. The results indicate a complex interaction between excision and subsequent drying and are discussed with a view of manipulating ROS production for optimisation of cryopreservation protocols.

  19. Identification of two hydrophilins that contribute to the desiccation and freezing tolerance of yeast (Saccharomyces cerevisiae) cells.

    PubMed

    Dang, Nghiem X; Hincha, Dirk K

    2011-06-01

    Hydrophilins are a group of proteins that are present in all organisms and that have been defined as being highly hydrophilic and rich in glycine. They are assumed to play important roles in cellular dehydration tolerance. There are 12 genes in the yeast Saccharomyces cerevisiae that encode hydrophilins and most of these genes are stress responsive. However, the functional role of yeast hydrophilins, especially in desiccation and freezing tolerance, is largely unknown. Here, we selected six candidate hydrophilins for further analysis. All six proteins were predicted to be intrinsically disordered, i.e. to have no stable structure in solution. The contribution of these proteins to the desiccation and freezing tolerance of yeast was investigated in the respective knock-out strains. Only the disruption of the genes YJL144W and YMR175W (SIP18) resulted in significantly reduced desiccation tolerance, while none of the strains was affected in its freezing tolerance under our experimental conditions. Complementation experiments showed that yeast cells overexpressing these two genes were both more desiccation and freezing tolerant, confirming the role of these two hydrophilins in yeast dehydration stress tolerance.

  20. Osmotic stress and recovery in field populations of Zygnema sp. (Zygnematophyceae, Streptophyta) on Svalbard (High Arctic) subjected to natural desiccation.

    PubMed

    Pichrtová, Martina; Hájek, Tomáš; Elster, Josef

    2014-08-01

    Zygnema is a genus of filamentous green algae belonging to the class of Zygnematophyceae (Streptophyta). In the Arctic, it typically forms extensive mats in habitats that regularly dry out during summer, and therefore, mechanisms of stress resistance are expected. We investigated its natural populations with respect to production of specialized desiccation-resistant cells and osmotic acclimation. Six populations in various stages of natural desiccation were selected, from wet biomass floating in water to dried paper-like crusts. After rewetting, plasmolysis and osmotic stress effects were studied using hypertonic sorbitol solutions, and the physiological state was estimated using chlorophyll a fluorescence parameters. All populations of Zygnema sp. formed stationary-phase cells filled with storage products. In green algal research, such cells are traditionally called akinetes. However, the populations differed in their reaction to osmotic stress. Whereas the wet-collected samples were strongly impaired, the osmotic stress resistance of the naturally dried samples was comparable to that of true aeroterrestrial algae. We showed that arctic populations of Zygnema acclimate well to natural desiccation via hardening that is mediated by slow desiccation. As no other types of specialized cells were observed, we assume that the naturally hardened akinetes also play a key role in winter survival. PMID:24476153