Hyperspectral sensing for turbid water quality monitoring in freshwater rivers: Empirical relationship between reflectance and turbidity and total solids.

PubMed

Wu, Jiunn-Lin; Ho, Chung-Ru; Huang, Chia-Ching; Srivastav, Arun Lal; Tzeng, Jing-Hua; Lin, Yao-Tung

2014-11-28

Total suspended solid (TSS) is an important water quality parameter. This study was conducted to test the feasibility of the band combination of hyperspectral sensing for inland turbid water monitoring in Taiwan. The field spectral reflectance in the Wu river basin of Taiwan was measured with a spectroradiometer; the water samples were collected from the different sites of the Wu river basin and some water quality parameters were analyzed on the sites (in situ) as well as brought to the laboratory for further analysis. To obtain the data set for this study, 160 in situ sample observations were carried out during campaigns from August to December, 2005. The water quality results were correlated with the reflectivity to determine the spectral characteristics and their relationship with turbidity and TSS. Furthermore, multiple-regression (MR) and artificial neural network (ANN) were used to model the transformation function between TSS concentration and turbidity levels of stream water, and the radiance measured by the spectroradiometer. The value of the turbidity and TSS correlation coefficient was 0.766, which implies that turbidity is significantly related to TSS in the Wu river basin. The results indicated that TSS and turbidity are positively correlated in a significant way across the entire spectrum, when TSS concentration and turbidity levels were under 800 mg·L(-1) and 600 NTU, respectively. Optimal wavelengths for the measurements of TSS and turbidity are found in the 700 and 900 nm range, respectively. Based on the results, better accuracy was obtained only when the ranges of turbidity and TSS concentration were less than 800 mg·L(-1) and less than 600 NTU, respectively and used rather than using whole dataset (R(2) = 0.93 versus 0.88 for turbidity and R(2) = 0.83 versus 0.58 for TSS). On the other hand, the ANN approach can improve the TSS retrieval using MR. The accuracy of TSS estimation applying ANN (R(2) = 0.66) was better than with the MR approach (R(2) = 0.58), as expected due to the nonlinear nature of the transformation model.

Hyperspectral Sensing for Turbid Water Quality Monitoring in Freshwater Rivers: Empirical Relationship between Reflectance and Turbidity and Total Solids

PubMed Central

Wu, Jiunn-Lin; Ho, Chung-Ru; Huang, Chia-Ching; Srivastav, Arun Lal; Tzeng, Jing-Hua; Lin, Yao-Tung

2014-01-01

Total suspended solid (TSS) is an important water quality parameter. This study was conducted to test the feasibility of the band combination of hyperspectral sensing for inland turbid water monitoring in Taiwan. The field spectral reflectance in the Wu river basin of Taiwan was measured with a spectroradiometer; the water samples were collected from the different sites of the Wu river basin and some water quality parameters were analyzed on the sites (in situ) as well as brought to the laboratory for further analysis. To obtain the data set for this study, 160 in situ sample observations were carried out during campaigns from August to December, 2005. The water quality results were correlated with the reflectivity to determine the spectral characteristics and their relationship with turbidity and TSS. Furthermore, multiple-regression (MR) and artificial neural network (ANN) were used to model the transformation function between TSS concentration and turbidity levels of stream water, and the radiance measured by the spectroradiometer. The value of the turbidity and TSS correlation coefficient was 0.766, which implies that turbidity is significantly related to TSS in the Wu river basin. The results indicated that TSS and turbidity are positively correlated in a significant way across the entire spectrum, when TSS concentration and turbidity levels were under 800 mg·L−1 and 600 NTU, respectively. Optimal wavelengths for the measurements of TSS and turbidity are found in the 700 and 900 nm range, respectively. Based on the results, better accuracy was obtained only when the ranges of turbidity and TSS concentration were less than 800 mg·L−1 and less than 600 NTU, respectively and used rather than using whole dataset (R2 = 0.93 versus 0.88 for turbidity and R2 = 0.83 versus 0.58 for TSS). On the other hand, the ANN approach can improve the TSS retrieval using MR. The accuracy of TSS estimation applying ANN (R2 = 0.66) was better than with the MR approach (R2 = 0.58), as expected due to the nonlinear nature of the transformation model. PMID:25460816

TSS concentration in sewers estimated from turbidity measurements by means of linear regression accounting for uncertainties in both variables.

PubMed

Bertrand-Krajewski, J L

2004-01-01

In order to replace traditional sampling and analysis techniques, turbidimeters can be used to estimate TSS concentration in sewers, by means of sensor and site specific empirical equations established by linear regression of on-site turbidity Tvalues with TSS concentrations C measured in corresponding samples. As the ordinary least-squares method is not able to account for measurement uncertainties in both T and C variables, an appropriate regression method is used to solve this difficulty and to evaluate correctly the uncertainty in TSS concentrations estimated from measured turbidity. The regression method is described, including detailed calculations of variances and covariance in the regression parameters. An example of application is given for a calibrated turbidimeter used in a combined sewer system, with data collected during three dry weather days. In order to show how the established regression could be used, an independent 24 hours long dry weather turbidity data series recorded at 2 min time interval is used, transformed into estimated TSS concentrations, and compared to TSS concentrations measured in samples. The comparison appears as satisfactory and suggests that turbidity measurements could replace traditional samples. Further developments, including wet weather periods and other types of sensors, are suggested.

Structure of the ent -Copalyl Diphosphate Synthase PtmT2 from Streptomyces platensis CB00739, a Bacterial Type II Diterpene Synthase

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rudolf, Jeffrey D.; Dong, Liao-Bin; Cao, Hongnan

Terpenoids are the largest and most structurally diverse family of natural products found in nature, yet their presence in bacteria is underappreciated. The carbon skeletons of terpenoids are generated through carbocation-dependent cyclization cascades catalyzed by terpene synthases (TSs). Type I and type II TSs initiate cyclization via diphosphate ionization and protonation, respectively, and protein structures of both types are known. Most plant diterpene synthases (DTSs) possess three alpha-helical domains (alpha beta gamma), which are thought to have arisen from the fusion of discrete, ancestral bacterial type I TSs (alpha) and type II TSs (beta gamma). Type II DTSs of bacterialmore » origin, of which there are no structurally characterized members, are a missing piece in the structural evolution of TSs. Here, we report the first crystal structure of a type II DTS from bacteria. PtnaT2 from Streptomyces platensis CB00739 was verified as an ent-copalyl diphosphate synthase involved in the biosynthesis of platensimycin and platencin. The crystal structure of PtmT2 was solved at a resolution of 1.80 angstrom, and docking studies suggest the catalytically active conformation of geranylgeranyl diphosphate (GGPP). Site-directed mutagenesis confirmed residues involved in binding the diphosphate moiety of GGPP and identified DxxxxE as a potential Mg2+-binding motif for type II DTSs of bacterial origin. Finally, both the shape and physicochemical properties of the active sites are responsible for determining specific catalytic outcomes of TSs. The structure of PtmT2 fundamentally advances the knowledge of bacterial TSs, their mechanisms, and their role in the evolution of TSs.« less

Structure of the ent-Copalyl Diphosphate Synthase PtmT2 from Streptomyces platensis CB00739, a Bacterial Type II Diterpene Synthase

PubMed Central

2016-01-01

Terpenoids are the largest and most structurally diverse family of natural products found in nature, yet their presence in bacteria is underappreciated. The carbon skeletons of terpenoids are generated through carbocation-dependent cyclization cascades catalyzed by terpene synthases (TSs). Type I and type II TSs initiate cyclization via diphosphate ionization and protonation, respectively, and protein structures of both types are known. Most plant diterpene synthases (DTSs) possess three α-helical domains (αβγ), which are thought to have arisen from the fusion of discrete, ancestral bacterial type I TSs (α) and type II TSs (βγ). Type II DTSs of bacterial origin, of which there are no structurally characterized members, are a missing piece in the structural evolution of TSs. Here, we report the first crystal structure of a type II DTS from bacteria. PtmT2 from Streptomyces platensis CB00739 was verified as an ent-copalyl diphosphate synthase involved in the biosynthesis of platensimycin and platencin. The crystal structure of PtmT2 was solved at a resolution of 1.80 Å, and docking studies suggest the catalytically active conformation of geranylgeranyl diphosphate (GGPP). Site-directed mutagenesis confirmed residues involved in binding the diphosphate moiety of GGPP and identified DxxxxE as a potential Mg2+-binding motif for type II DTSs of bacterial origin. Finally, both the shape and physicochemical properties of the active sites are responsible for determining specific catalytic outcomes of TSs. The structure of PtmT2 fundamentally advances the knowledge of bacterial TSs, their mechanisms, and their role in the evolution of TSs. PMID:27490479

Structure of the ent-Copalyl Diphosphate Synthase PtmT2 from Streptomyces platensis CB00739, a Bacterial Type II Diterpene Synthase.

PubMed

Rudolf, Jeffrey D; Dong, Liao-Bin; Cao, Hongnan; Hatzos-Skintges, Catherine; Osipiuk, Jerzy; Endres, Michael; Chang, Chin-Yuan; Ma, Ming; Babnigg, Gyorgy; Joachimiak, Andrzej; Phillips, George N; Shen, Ben

2016-08-31

Terpenoids are the largest and most structurally diverse family of natural products found in nature, yet their presence in bacteria is underappreciated. The carbon skeletons of terpenoids are generated through carbocation-dependent cyclization cascades catalyzed by terpene synthases (TSs). Type I and type II TSs initiate cyclization via diphosphate ionization and protonation, respectively, and protein structures of both types are known. Most plant diterpene synthases (DTSs) possess three α-helical domains (αβγ), which are thought to have arisen from the fusion of discrete, ancestral bacterial type I TSs (α) and type II TSs (βγ). Type II DTSs of bacterial origin, of which there are no structurally characterized members, are a missing piece in the structural evolution of TSs. Here, we report the first crystal structure of a type II DTS from bacteria. PtmT2 from Streptomyces platensis CB00739 was verified as an ent-copalyl diphosphate synthase involved in the biosynthesis of platensimycin and platencin. The crystal structure of PtmT2 was solved at a resolution of 1.80 Å, and docking studies suggest the catalytically active conformation of geranylgeranyl diphosphate (GGPP). Site-directed mutagenesis confirmed residues involved in binding the diphosphate moiety of GGPP and identified DxxxxE as a potential Mg(2+)-binding motif for type II DTSs of bacterial origin. Finally, both the shape and physicochemical properties of the active sites are responsible for determining specific catalytic outcomes of TSs. The structure of PtmT2 fundamentally advances the knowledge of bacterial TSs, their mechanisms, and their role in the evolution of TSs.

40 CFR 424.15 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... With Wet Air Pollution Control Devices Subcategory § 424.15 Standards of performance for new sources... values for 30 consecutive days shall not exceed— Metric units (kg/Mwh) TSS 0.024 0.012 Chromium total .0008 .0004 Chromium VI .00008 .00004 Manganese total .008 .0039 pH (1) (1) English units (lb/Mwh) TSS...

Evaluation of compost/mulch as highway embankment erosion control in Louisiana at the plot-scale

NASA Astrophysics Data System (ADS)

Bakr, Noura; Weindorf, David C.; Zhu, Yuanda; Arceneaux, Allen E.; Selim, H. M.

2012-10-01

SummaryTotal suspended solids (TSS) and associated turbidity in runoff water are considered the most problematic nonpoint source pollutant of Louisiana surface waters. With high precipitation in Louisiana, attention should be given to controlling highway right-of-way erosion. The use of compost/mulch for erosion control enhances soil conservation and substantially reduces erosion. The main objective of this study was to assess the effect of compost/mulch placement on runoff water quality on roadsides. Our hypothesis was that the use of compost/mulch would significantly reduce TSS and turbidity in runoff from highway right-of-ways in Louisiana. Two locations constituting four sites and eight individual plots were chosen; one in an active highway construction area and another in an established area plagued by continual rill and sheet erosion. Thicknesses of compost/mulch (5 and 10 cm), slope inclination (10-34%), and tillage practices (till vs. no-till) were evaluated. Runoff, triggered by storm water events, was collected using ISCO auto-samplers from June 2010 to August 2011 and the samples were analyzed for TSS, turbidity, biochemical oxygen demand, electrical conductivity, and pH. The results of factor analysis showed that the compost/mulch thickness was the most influential variable affecting water quality. Two samples t-test results indicated that TSS and turbidity were significantly different across all comparative variables; construction activities, compost/mulch applications, and tillage practices. The results confirmed the effectiveness of compost/mulch cover as a successful best management practice. Specifically decreases in TSS of 70% and 74% were achieved for the 5 cm and 10 cm compost/mulch application when compared to no compost/mulch, respectively. Light tillage application increased TSS as much as 67%. Therefore, light tillage is not recommended since it decreased the effectiveness of compost/mulch in reducing runoff and sediment losses.

Toxic shock syndrome in children: epidemiology, pathogenesis, and management.

PubMed

Chuang, Yu-Yu; Huang, Yhu-Chering; Lin, Tzou-Yien

2005-01-01

Toxic shock syndrome (TSS) is an acute, toxin-mediated illness, like endotoxic shock, and is characterized by fever, rash, hypotension, multiorgan involvement, and desquamation. TSS reflects the most severe form of the disease caused by Staphylococcus aureus and Streptococcus pyogenes. A case definition for staphylococcal TSS was well established in the early 1980s and helped in defining the epidemiology. Since the late 1980s, a resurgence of highly invasive streptococcal infections, including a toxic shock-like syndrome, was noted worldwide and a consensus case definition for streptococcal TSS was subsequently proposed in 1993. Both TSS and the toxic shock-like syndrome occur at a lower incidence in children than in adults. Changes in the manufacturing and use of tampons led to a decline in staphylococcal TSS over the past decade, while the incidence of nonmenstrual staphylococcal TSS increased. Nonmenstrual TSS and menstrual TSS are now reported with almost equal frequency. The incidence of streptococcal TSS remains constant after its resurgence, but varies with geographic location. Streptococcal TSS occurs most commonly following varicella or during the use of NSAIDs. Sites of infection in streptococcal TSS are much deeper than in staphylococcal TSS, such as infection caused by blunt trauma, and necrotizing fasciitis. Bacteremia is more common in streptococcal TSS than in staphylococcal TSS. Mortality associated with streptococcal TSS is 5-10% in children, much lower than in adults (30-80%), and is 3-5% for staphylococcal TSS in children.TSS is thought to be a superantigen-mediated disease. Toxins produced by staphylococci and streptococci act as superantigens that can activate the immune system by bypassing the usual antigen-mediated immune-response sequence. The host-pathogen interaction, virulence factors, and the absence or presence of host immunity determines the epidemiology, clinical syndrome, and outcome. Early recognition of this disease is important, because the clinical course is fulminant and the outcome depends on the prompt institution of therapy. Management of a child with TSS includes hemodynamic stabilization and appropriate antimicrobial therapy to eradicate the bacteria. Supportive therapy, aggressive fluid resuscitation, and vasopressors remain the main elements. An adjuvant therapeutic strategy may include agents that can block superantigens, such as intravenous immunoglobulin that contains superantigen neutralizing antibodies.

Suspended-sediment concentrations, loads, total suspended solids, turbidity, and particle-size fractions for selected rivers in Minnesota, 2007 through 2011

USGS Publications Warehouse

Ellison, Christopher A.; Savage, Brett E.; Johnson, Gregory D.

2014-01-01

Sediment-laden rivers and streams pose substantial environmental and economic challenges. Excessive sediment transport in rivers causes problems for flood control, soil conservation, irrigation, aquatic health, and navigation, and transports harmful contaminants like organic chemicals and eutrophication-causing nutrients. In Minnesota, more than 5,800 miles of streams are identified as impaired by the Minnesota Pollution Control Agency (MPCA) due to elevated levels of suspended sediment. The U.S. Geological Survey, in cooperation with the MPCA, established a sediment monitoring network in 2007 and began systematic sampling of suspended-sediment concentrations (SSC), total suspended solids (TSS), and turbidity in rivers across Minnesota to improve the understanding of fluvial sediment transport relations. Suspended-sediment samples collected from 14 sites from 2007 through 2011 indicated that the Zumbro River at Kellogg in the driftless region of southeast Minnesota had the highest mean SSC of 226 milligrams per liter (mg/L) followed by the Minnesota River at Mankato with a mean SSC of 193 mg/L. During the 2011 spring runoff, the single highest SSC of 1,250 mg/L was measured at the Zumbro River. The lowest mean SSC of 21 mg/L was measured at Rice Creek in the northern Minneapolis- St. Paul metropolitan area. Total suspended solids (TSS) have been used as a measure of fluvial sediment by the MPCA since the early 1970s; however, TSS concentrations have been determined to underrepresent the amount of suspended sediment. Because of this, the MPCA was interested in quantifying the differences between SSC and TSS in different parts of the State. Comparisons between concurrently sampled SSC and TSS indicated significant differences at every site, with SSC on average two times larger than TSS concentrations. The largest percent difference between SSC and TSS was measured at the South Branch Buffalo River at Sabin, and the smallest difference was observed at the Des Moines River at Jackson. Regression analysis indicated that 7 out of 14 sites had poor or no relation between SSC and streamflow. Only two sites, the Knife River and the Wild Rice River at Twin Valley, had strong correlations between SSC and streamflow, with coefficient of determination (R2) values of 0.82 and 0.80, respectively. In contrast, turbidity had moderate to strong relations with SSC at 10 of 14 sites and was superior to streamflow for estimating SSC at all sites. These results indicate that turbidity may be beneficial as a surrogate for SSC in many of Minnesota’s rivers. Suspended-sediment loads and annual basin yields indicated that the Minnesota River had the largest average annual sediment load of 1.8 million tons per year and the largest mean annual sediment basin yield of 120 tons of sediment per year per square mile. Annual TSS loads were considerably lower than suspended-sediment loads. Overall, the largest suspended-sediment and TSS loads were transported during spring snowmelt runoff, although loads during the fall and summer seasons occasionally exceeded spring runoff at some sites. This study provided data from which to characterize suspended sediment across Minnesota’s diverse geographical settings. The data analysis improves understanding of sediment transport relations, provides information for improving sediment budgets, and documents baseline data to aid in understanding the effects of future land use/land cover on water quality. Additionally, the data provides insight from which to evaluate the effectiveness and efficiency of best management practices at the watershed scale.

Laboratory upwelled radiance and reflectance spectra of Kerr reservoir sediment waters

NASA Technical Reports Server (NTRS)

Witte, W. G.; Whitlock, C. H.; Morris, W. D.; Gurganus, E. A.

1982-01-01

Reflectance, chromaticity, and several other physical and chemical properties were measured for various water mixtures of bottom sediments taken from two sites at Kerr Reservoir, Virginia. Mixture concentrations ranged from 5 to 1000 ppm by weight of total suspended solids (TSS) in filtered deionized tap water. The two sets of radiance and reflectance spectra obtained were similar in shape and magnitude for comparable values of TSS. Upwelled reflectance was observed to be a nonlinear function of TSS with the degree of curvature a function of wavelength. Sediment from the downstream site contained a greater amount of particulate organic carbon than from the upstream site. No strong conclusions can be made regarding the effects of this difference on the radiance and reflectance spectra. Near-infrared wavelengths appear useful for measuring highly turbid water with concentrations up to 1000 ppm or more. Chromaticity characteristics do not appear useful for monitoring sediment loads above 150 ppm.

Fort Dix Remedial Investigation/Feasibility Study for 13 Sites, Final Technical Plan, Data Item A004

DTIC Science & Technology

1995-09-01

39 oxygen demand (COD), TSS, total dissolved solids ( TDS ), nitrate/nitrite, sulfate, W0109314.M80 7133-04 5-4 SECTION 5 phosphateand alkalinity...TSS, TDS , BOD-5, COD, alkalinity, hardness, 38 and gross alpha, beta, and gamma radiation (Table 2). 39 W0109314.M80 12-2 7133-°4 SECTION 12 l...wells. Groundwater samples 28 will be analyzed for TCL VOCs, TCL SVOCs, TAL metals (nonfiltered and filtered) 29 TSS, TDS , BOD-5, COD, alkalinity

40 CFR 417.166 - Pretreatment standards for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... pollutant property Pretreatment standard BOD5 No limitation. COD Do. TSS Do. Surfactants Do. Oil and grease... No limitation. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. [40 FR 27455, June 30, 1975...

40 CFR 417.166 - Pretreatment standards for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... pollutant property Pretreatment standard BOD5 No limitation. COD Do. TSS Do. Surfactants Do. Oil and grease... No limitation. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. [40 FR 27455, June 30, 1975...

40 CFR 417.166 - Pretreatment standards for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... pollutant property Pretreatment standard BOD5 No limitation. COD Do. TSS Do. Surfactants Do. Oil and grease... No limitation. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. [40 FR 27455, June 30, 1975...

40 CFR 417.166 - Pretreatment standards for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... pollutant property Pretreatment standard BOD5 No limitation. COD Do. TSS Do. Surfactants Do. Oil and grease... No limitation. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. [40 FR 27455, June 30, 1975...

Concentration-discharge responses to storm events in coastal California watersheds

NASA Astrophysics Data System (ADS)

Aguilera, R.; Melack, J. M.

2017-12-01

Storm events in montane catchments are the main cause of mobilization of solutes and particulates into and within stream channels in coastal California. Non-linear behavior of nutrients and suspended sediments during storms is evident in the hysteresis that arises in concentration-discharge (C-Q) relationships. We examined patterns in the C-Q hysteresis of nutrients (NO3-, NH4+, DON and PO43-) and total suspended solids (TSS) during storms across ten sites and water years 2002 to 2015 by quantifying the slope of the C-Q relationship and the rotational pattern of the hysteresis loop. We observed several hysteresis types: constituents associated with sediment transport (PO43- and TSS) were flushed during storm events, whereas nitrogen species had hysteretic responses such as dilution with clockwise rotation in urban sites and enrichment with anti-clockwise rotation in undeveloped sites. The wide range of C-Q responses that occurred among sites and seasons reflected the variable hydrological and biogeochemical characteristics of catchments and storms. Storm responses for nitrate in nested catchments differed in slope and rotation of C-Q hysteresis. Upland undeveloped and lowland urban sites had anti-clockwise rotation at the onset of the rainy season following a dry year, which implied a delay in the transport of this solute to the streams. By the middle of the season, the urban site switched from dilution to enrichment, and then again to dilution with clockwise rotation, which implied high initial concentrations and proximal sources by the end of the season.

40 CFR 406.65 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice Processing Subcategory... units (kilograms per 1,000 kg of rice) BOD5 0.21 0.07 TSS 0.09 .03 pH (1) (1) English units (pounds per hundredweight of rice) BOD5 0.021 0.007 TSS 0.009 .003 pH (1) (1) 1 Within the range 6.0 to 9.0. ...

Streamflow and water-quality properties in the West Fork San Jacinto River Basin and regression models to estimate real-time suspended-sediment and total suspended-solids concentrations and loads in the West Fork San Jacinto River in the vicinity of Conroe, Texas, July 2008-August 2009

USGS Publications Warehouse

Bodkin, Lee J.; Oden, Jeannette H.

2010-01-01

To better understand the hydrology (streamflow and water quality) of the West Fork San Jacinto River Basin downstream from Lake Conroe near Conroe, Texas, including spatial and temporal variation in suspended-sediment (SS) and total suspended-solids (TSS) concentrations and loads, the U.S. Geological Survey, in cooperation with the Houston-Galveston Area Council and the Texas Commission on Environmental Quality, measured streamflow and collected continuous and discrete water-quality data during July 2008-August 2009 in the West Fork San Jacinto River Basin downstream from Lake Conroe. During July 2008-August 2009, discrete samples were collected and streamflow measurements were made over the range of flow conditions at two streamflow-gaging stations on the West Fork San Jacinto River: West Fork San Jacinto River below Lake Conroe near Conroe, Texas (station 08067650) and West Fork San Jacinto River near Conroe, Texas (station 08068000). In addition to samples collected at these two main monitoring sites, discrete sediment samples were also collected at five additional monitoring sites to help characterize water quality in the West Fork San Jacinto River Basin. Discrete samples were collected semimonthly, regardless of flow conditions, and during periods of high flow resulting from storms or releases from Lake Conroe. Because the period of data collection was relatively short (14 months) and low flow was prevalent during much of the study, relatively few samples collected were representative of the middle and upper ranges of historical daily mean streamflows. The largest streamflows tended to occur in response to large rainfall events and generally were associated with the largest SS and TSS concentrations. The maximum SS and TSS concentrations at station 08067650 (180 and 133 milligrams per liter [mg/L], respectively) were on April 19, 2009, when the instantaneous streamflow was the third largest associated with a discrete sample at the station. SS concentrations were 25 mg/L or less in 26 of 29 environmental samples and TSS concentrations were 25 mg/L or less in 25 of 28 environmental samples. Median SS and TSS concentrations were 7.0 and 7.6 mg/L, respectively. At station 08068000, the maximum SS concentration (1,270 mg/L) was on April 19, 2009, and the maximum TSS concentration (268 mg/L) was on September 18, 2008. SS concentrations were 25 mg/L or less in 16 of 27 of environmental samples and TSS concentrations were 25 mg/L or less in 18 of 26 environmental samples at the station. Median SS and TSS concentrations were 18.0 and 14.0 mg/L, respectively. The maximum SS and TSS concentrations for all five additional monitoring sites were 3,110 and 390 mg/L, respectively, and the minimum SS and TSS concentrations were 5.0 and 1.0 mg/L, respectively. Median concentrations ranged from 14.0 to 54.0 mg/L for SS and from 11.0 to 14.0 mg/L for TSS. Continuous measurements of streamflow and selected water-quality properties at stations 08067650 and 08068000 were evaluated as possible variables in regression equations developed to estimate SS and TSS concentrations and loads. Surrogate regression equations were developed to estimate SS and TSS loads by using real-time turbidity and streamflow data; turbidity and streamflow resulted in the best regression models for estimating near real-time SS and TSS concentrations for stations 08097650 and 08068000. Relatively large errors are associated with the regression-computed SS and TSS concentrations; the 90-percent prediction intervals for SS and TSS concentrations were (+/-)48.9 and (+/-)43.2 percent, respectively, for station 08067650 and (+/-)47.7 and (+/-)43.2 percent, respectively, for station 08068000. Regression-computed SS and TSS concentrations were corrected for bias before being used to compute SS and TSS loads. The total estimated SS and TSS loads during July 2008-August 2009 were about 3,540 and 1,900 tons, respectively, at station 08067650 and about 156,000 an

40 CFR 424.35 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

40 CFR 424.35 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

40 CFR 424.35 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

40 CFR 432.85 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

..., fecal coliform, O&G, and TSS specified in § 432.82(a). (b) Facilities that generate more than 50 million pounds per year of finished products must achieve the limitations for BOD5, fecal coliform, O&G, and TSS...

40 CFR 432.65 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

..., fecal coliform, O&G, and TSS specified in § 432.62(a). (b) Facilities that generate more than 50 million pounds per year of finished products must achieve the limitations for BOD5, fecal coliform, O&G, and TSS...

40 CFR 432.85 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

..., fecal coliform, O&G, and TSS specified in § 432.82(a). (b) Facilities that generate more than 50 million pounds per year of finished products must achieve the limitations for BOD5, fecal coliform, O&G, and TSS...

40 CFR 424.35 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

40 CFR 424.35 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

...— Metric units (kg/kkg processed) TSS 0.271 0.136 Chromium total .0054 .0027 Manganese total 0.054 .027 pH (1) (1) English units (lb/ton processed) TSS .542 .271 Chromium total .011 .0054 Manganese total .108...

Global Transcriptional Start Site Mapping Using Differential RNA Sequencing Reveals Novel Antisense RNAs in Escherichia coli

PubMed Central

Thomason, Maureen K.; Bischler, Thorsten; Eisenbart, Sara K.; Förstner, Konrad U.; Zhang, Aixia; Herbig, Alexander; Nieselt, Kay

2014-01-01

While the model organism Escherichia coli has been the subject of intense study for decades, the full complement of its RNAs is only now being examined. Here we describe a survey of the E. coli transcriptome carried out using a differential RNA sequencing (dRNA-seq) approach, which can distinguish between primary and processed transcripts, and an automated prediction algorithm for transcriptional start sites (TSS). With the criterion of expression under at least one of three growth conditions examined, we predicted 14,868 TSS candidates, including 5,574 internal to annotated genes (iTSS) and 5,495 TSS corresponding to potential antisense RNAs (asRNAs). We examined expression of 14 candidate asRNAs by Northern analysis using RNA from wild-type E. coli and from strains defective for RNases III and E, two RNases reported to be involved in asRNA processing. Interestingly, nine asRNAs detected as distinct bands by Northern analysis were differentially affected by the rnc and rne mutations. We also compared our asRNA candidates with previously published asRNA annotations from RNA-seq data and discuss the challenges associated with these cross-comparisons. Our global transcriptional start site map represents a valuable resource for identification of transcription start sites, promoters, and novel transcripts in E. coli and is easily accessible, together with the cDNA coverage plots, in an online genome browser. PMID:25266388

Comparability of river suspended-sediment sampling and laboratory analysis methods

USGS Publications Warehouse

Groten, Joel T.; Johnson, Gregory D.

2018-03-06

Accurate measurements of suspended sediment, a leading water-quality impairment in many Minnesota rivers, are important for managing and protecting water resources; however, water-quality standards for suspended sediment in Minnesota are based on grab field sampling and total suspended solids (TSS) laboratory analysis methods that have underrepresented concentrations of suspended sediment in rivers compared to U.S. Geological Survey equal-width-increment or equal-discharge-increment (EWDI) field sampling and suspended sediment concentration (SSC) laboratory analysis methods. Because of this underrepresentation, the U.S. Geological Survey, in collaboration with the Minnesota Pollution Control Agency, collected concurrent grab and EWDI samples at eight sites to compare results obtained using different combinations of field sampling and laboratory analysis methods.Study results determined that grab field sampling and TSS laboratory analysis results were biased substantially low compared to EWDI sampling and SSC laboratory analysis results, respectively. Differences in both field sampling and laboratory analysis methods caused grab and TSS methods to be biased substantially low. The difference in laboratory analysis methods was slightly greater than field sampling methods.Sand-sized particles had a strong effect on the comparability of the field sampling and laboratory analysis methods. These results indicated that grab field sampling and TSS laboratory analysis methods fail to capture most of the sand being transported by the stream. The results indicate there is less of a difference among samples collected with grab field sampling and analyzed for TSS and concentration of fines in SSC. Even though differences are present, the presence of strong correlations between SSC and TSS concentrations provides the opportunity to develop site specific relations to address transport processes not captured by grab field sampling and TSS laboratory analysis methods.

Odors from Sewage Sludge and Livestock: Associations with Self-Reported Health

PubMed Central

Lowman, Amy; Keil, Alex; Marshall, Stephen W.

2014-01-01

Objectives Class B treated sewage sludge (TSS) contains microbes and toxicants and is applied to land in areas where livestock wastes may be present. We evaluated relationships of reports of TSS and livestock odors with acute symptoms and excessive flies. Methods A total of 158 adults living near liquid TSS application sites, 85 living near cake TSS application sites, and 188 living in comparison areas responded to a household survey regarding odors, health, and demographics. We identified symptom groups using factor analysis. We used generalized estimating equations to fit linear models for associations between factor scores and odors, and Poisson models for associations with specific symptoms. Results Most factor scores were similar between exposure groups. Covariate-adjusted z-scores for lower respiratory symptoms were 0.28 (95% confidence interval [CI] –0.10, 0.65) higher among residents who reported moderate to very strong liquid TSS odor than among residents in comparison areas, and 0.28 (95% CI 0.05, 0.50) higher among residents who reported moderate to very strong livestock odor compared with residents reporting no or faint livestock odor. The factor score for dermatologic conditions was higher among residents who reported higher liquid sludge odor (0.27, 95% CI –0.13, 0.68), primarily due to skin rash (prevalence ratio = 2.21, 95% CI 1.13, 4.32). Excessive flies were reported twice as commonly among respondents who reported moderate to very strong TSS odor than among other residents. Conclusions Reported odors from TSS and livestock were associated with some acute symptoms. Health departments should monitor land applications of human and animal wastes and conduct surveillance of health problems reported by neighbors. PMID:25364052

40 CFR 432.75 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... achieve the standards for BOD5, fecal coliform, O&G, and TSS specified in § 432.72(a). (b) Facilities that..., fecal coliform, O&G, and TSS specified in § 432.72(b) and the limitations for ammonia (as N) and total...

40 CFR 432.95 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... standards for BOD 5, fecal coliform, O&G, and TSS specified in § 432.92(a). (b) Facilities that generate... coliform, O&G, and TSS specified in § 432.92(b) and the limitations for ammonia (as N) and total nitrogen...

40 CFR 471.53 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... 4.29 TSS 6.44 5.15 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (c) Drawing spent... Nickel 0.655 0.441 Fluoride 70.8 31.4 Molybdenum 5.99 2.66 Oil and grease 11.9 11.9 TSS 17.9 14.3 pH (1... and grease 2.81 2.81 TSS 4.22 3.37 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (j...

40 CFR 471.53 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... 4.29 TSS 6.44 5.15 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (c) Drawing spent... Nickel 0.655 0.441 Fluoride 70.8 31.4 Molybdenum 5.99 2.66 Oil and grease 11.9 11.9 TSS 17.9 14.3 pH (1... and grease 2.81 2.81 TSS 4.22 3.37 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (j...

40 CFR 406.25 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling Subcategory § 406.25... (kilograms per 1,000 kg of corn) BOD5 0.11 0.036 TSS 0.054 0.18 pH (1) (1) English units (pounds per 1,000 stdbu of corn) BOD5 6.0 2.0 TSS 3.0 1.0 pH (1) (1) 1 Within the range 6.0 to 9.0. ...

40 CFR 406.15 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Wet Milling Subcategory § 406.15... (kilograms per 1,000 kg of corn) BOD5 1.08 0.36 TSS 1.35 .45 pH (1) (1) English units (pounds per 1,000 stdbu of corn) BOD5 60 20 TSS 75 25 pH (1) (1) 1 Within the range 6.0 to 9.0. [41 FR 50823, Nov. 18, 1976] ...

40 CFR 406.105 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Wheat Starch and Gluten Subcategory... units (kg/kkg of raw material (wheat flour)) BOD5 3.0 1.0 TSS 3.0 1.0 pH (1) (1) English units (lb/1,000 lb of raw material (wheat flour)) BOD5 3.0 1.0 TSS 3.0 1.0 pH (1) (1) 1 Within the range 6.0 to 9.0. ...

Trace metals partitioning between particulate and dissolved phases along a tropical mangrove estuary (Can Gio, Vietnam).

PubMed

Thanh-Nho, Nguyen; Strady, Emilie; Nhu-Trang, Tran-Thi; David, Frank; Marchand, Cyril

2018-04-01

Mangroves can be considered as biogeochemical reactors along (sub)tropical coastlines, acting both as sinks or sources for trace metals depending on environmental factors. In this study, we characterized the role of a mangrove estuary, developing downstream a densely populated megacity (Ho Chi Minh City, Vietnam), on the fate and partitioning of trace metals. Surface water and suspended particulate matter were collected at four sites along the estuarine salinity gradient during 24 h cycling in dry and rainy seasons. Salinity, pH, DO, TSS, POC, DOC, dissolved and particulate Fe, Mn, Cr, As, Cu, Ni, Co and Pb were measured. TSS was the main trace metals carrier during their transit in the estuary. However, TSS variations did not explain the whole variability of metals distribution. Mn, Cr and As were highly reactive metals while the other metals (Fe, Ni, Cu, Co and Pb) presented stable log K D values along the estuary. Organic matter dynamic appeared to play a key role in metals fractioning. Its decomposition during water transit in the estuary induced metal desorption, especially for Cr and As. Conversely, dissolved Mn concentrations decreased along the estuary, which was suggested to result from Mn oxidative precipitation onto solid phase due to oxidation and pH changes. Extra sources as pore-water release, runoff from adjacent soils, or aquaculture effluents were suggested to be involved in trace metal dynamic in this estuary. In addition, the monsoon increased metal loads, notably dissolved and particulate Fe, Cr, Ni and Pb. Copyright © 2018 Elsevier Ltd. All rights reserved.

Suspended sediment projections in Apalachicola Bay in response to altered river flow and sediment loads under climate change and sea level rise

NASA Astrophysics Data System (ADS)

Huang, Wenrui; Hagen, Scott C.; Wang, Dingbao; Hovenga, Paige A.; Teng, Fei; Weishampel, John F.

2016-10-01

Suspended sediments, or total suspended solids (TSS), are an important factor for oyster habitat. While high concentrations of suspended sediments can cause a reduction of oyster density, some level of suspended sediment is required to supply oysters with necessary nutrients. In this study, characteristics of TSS variations in response to sea level rise (SLR) at two oyster reefs in Apalachicola Bay are investigated by coupled estuarine hydrodynamic and sediment transport modeling. A storm event in 1993 and a year-long period in 2010 under recent sea level conditions are selected as the baseline conditions. Scenarios of river flow and sediment loads under SLR and climate change are obtained by downscaled global climate modeling. Compared to the baseline conditions, simulations of TSS indicate that predicted SLR yields a substantial decrease in TSS near the two oyster reefs. However, TSS levels differed at the two study locations. TSS changes by SLR revealed minimal impact on oyster habitat at the Dry Bar site (to the west of the mouth of the Apalachicola River) but are projected to have a significant impact at the Cat Point site (to the east of the Apalachicola River). At Cat Point, because SLR causes the increase of salt water intrusion from the Gulf through a large tidal inlet (East Pass), maximum sediment concentration is near zero for 0.2-m SLR and equal to zero for 0.5- and 1.2-m SLR. Therefore, SLR may result in a substantial loss of nutrients from suspended sediment in the oyster reef at Cat Point.

Global transcriptional start site mapping using differential RNA sequencing reveals novel antisense RNAs in Escherichia coli.

PubMed

Thomason, Maureen K; Bischler, Thorsten; Eisenbart, Sara K; Förstner, Konrad U; Zhang, Aixia; Herbig, Alexander; Nieselt, Kay; Sharma, Cynthia M; Storz, Gisela

2015-01-01

While the model organism Escherichia coli has been the subject of intense study for decades, the full complement of its RNAs is only now being examined. Here we describe a survey of the E. coli transcriptome carried out using a differential RNA sequencing (dRNA-seq) approach, which can distinguish between primary and processed transcripts, and an automated prediction algorithm for transcriptional start sites (TSS). With the criterion of expression under at least one of three growth conditions examined, we predicted 14,868 TSS candidates, including 5,574 internal to annotated genes (iTSS) and 5,495 TSS corresponding to potential antisense RNAs (asRNAs). We examined expression of 14 candidate asRNAs by Northern analysis using RNA from wild-type E. coli and from strains defective for RNases III and E, two RNases reported to be involved in asRNA processing. Interestingly, nine asRNAs detected as distinct bands by Northern analysis were differentially affected by the rnc and rne mutations. We also compared our asRNA candidates with previously published asRNA annotations from RNA-seq data and discuss the challenges associated with these cross-comparisons. Our global transcriptional start site map represents a valuable resource for identification of transcription start sites, promoters, and novel transcripts in E. coli and is easily accessible, together with the cDNA coverage plots, in an online genome browser. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Assessment of the contribution of sewer deposits to suspended solids loads in combined sewer systems during rain events.

PubMed

Hannouche, A; Chebbo, G; Joannis, C

2014-04-01

Within the French observatories network SOERE "URBIS," databases of continuous turbidity measurements accumulating hundreds of events and many dry weather days are available for two sites with different features (Clichy in Paris and Ecully in Lyon). These measurements, converted into total suspended solids (TSS) concentration using TSS-turbidity relationships and combined with a model of runoff event mean concentration, enable the assessment of the contribution of sewer deposits to wet weather TSS loads observed at the outlet of the two watersheds. Results show that the contribution of sewer deposits to wet weather suspended solid's discharges is important but variable (between 20 and 80 % of the mass at the outlet depending on the event), including a site allegedly free of (coarse) sewer deposits. The uncertainties associated to these results are assessed too.

Concentration-Discharge Responses to Storm Events in Coastal California Watersheds

NASA Astrophysics Data System (ADS)

Aguilera, Rosana; Melack, John M.

2018-01-01

Storm events in montane catchments are the main cause of mobilization of solutes and particulates into and within stream channels in coastal California. Nonlinear behavior of nutrients and suspended sediments during storms is evident in the hysteresis that arises in concentration-discharge (C-Q) relationships. We examined patterns in the C-Q hysteresis of nutrients (NO3-, NH4+, DON, and PO43-) and total suspended solids (TSS) during storms across 10 sites and water years 2002-2015 by quantifying the slope of the C-Q relationship and the rotational pattern of the hysteresis loop. We observed several hysteresis types in the ˜400 storms included in our study. Concentrations of constituents associated with sediment transport (PO43- and TSS) peaked during high flows. Conversely, nitrogen species had hysteretic responses such as dilution with clockwise rotation in urban sites and enrichment with anticlockwise rotation in undeveloped sites. The wide range of C-Q responses that occurred among sites and seasons reflected the variable hydrological and biogeochemical characteristics of catchments and storms. Responses for nitrate in nested catchments differed in slope and rotation of C-Q hysteresis. Upland undeveloped and lowland urban sites had anticlockwise rotation at the onset of the rainy season following a dry year, which implied a delay in the transport of this solute to the streams. Slopes by the middle of the rainy season showed that the urban site switched from dilution to enrichment, and then again to dilution with clockwise rotation at the end of the season, which implied high initial concentrations and proximal sources.

Quantifying suspended solids in small rivers using satellite data.

PubMed

Isidro, Celso M; McIntyre, Neil; Lechner, Alex M; Callow, Ian

2018-09-01

The management of suspended solids and associated contaminants in rivers requires knowledge of sediment sources. In-situ sampling can only describe the integrated impact of the upstream sources. Empirical models that use surface reflectance from satellite images to estimate total suspended solid (TSS) concentrations can be used to supplement measurements and provide spatially continuous maps. However, there are few examples, especially in narrow, shallow and hydrologically dynamic rivers found in mountainous areas. A case study of the Didipio catchment in Philippines was used to address these issues. Four 5-m resolution RapidEye images, from between the years 2014 and 2016, and near-simultaneous ground measurements of TSS concentrations were used to develop a power law model that approximates the relationship between TSS and reflectance for each of four spectral bands. A second dataset using two 2-m resolution Pleiades-1A and a third using a 6-m resolution SPOT-6 image along with ground-based measurements, were consistent with the model when using the red band data. Using that model, encompassing data from all three datasets, gave an R 2 value of 65% and a root mean square error of 519mgL -1 . A linear relationship between reflectance and TSS exists from 1mgL -1 to approximately 500mgL -1 . In contrast, for TSS measurements between 500mgL -1 and 3580mgL -1 reflectance increases at a generally lower and more variable rate. The results were not sensitive to changing the pixel location within the vicinity of the ground sampling location. The model was used to generate a continuous map of TSS concentration within the catchment. Further ground-based measurements including TSS concentrations that are higher than 3580mgL -1 would allow the model to be developed and applied more confidently over the full relevant range of TSS. Copyright © 2018 Elsevier B.V. All rights reserved.

Resiliency of the western Chesapeake Bay to total suspended solid concentrations following storms and accounting for land-cover

NASA Astrophysics Data System (ADS)

Hasan, Mejs; Benninger, Larry

2017-05-01

The effect of storms on water quality in the Chesapeake Bay has been studied in a patchwork fashion at various sites for short periods of time. In this paper, we use a relationship between MODIS-Terra red light reflectance and total suspended solid (TSS) concentrations in order to develop a fourteen-year time period of TSS estimates during which effects of storms and land cover can be studied over a large portion of the Bay. Exponential fits were most appropriate, resulting in viable reflectance-TSS relationships for the five major Western Shore rivers and the Mainstem of the Bay. Other tributaries were less well-disposed for such relationships due to lack of a large range of TSS concentrations, shallow river beds, or low number of data points. Treating the entire Chesapeake as a single entity and modeling a single reflectance-TSS relationship for the entire estuary produced poorer models with less significance compared to treating each channel separately. Over 2800 rain events were studied in the Lower Western Shore between 2000 and 2014. We found some evidence that higher rainfall amounts correspond to a lower distribution of TSS concentrations 1 day following the event in forested watersheds. At rainfall events of <50 mm, maximum TSS within one day of the storm was highly variable, suggesting that rainfall amounts alone cannot explain variation in TSS levels. Finally, we found value in the use of prediction intervals around TSS estimates, a statistical procedure uncommonly used heretofore with satellite-based estimates but which can help to determine if results are significant or not.

Water-quality characteristics of stormwater runoff in Rapid City, South Dakota, 2008-14

USGS Publications Warehouse

Hoogestraat, Galen K.

2015-01-01

For the Arrowhead and Meade-Hawthorne sites, event-mean concentrations typically exceeded the TSS and bacteria beneficial-use criteria for Rapid Creek by 1–2 orders of magnitude. Comparing the two drainage basins, median TSS event-mean concentrations were more than two times greater at the Meade-Hawthorne outlet (520 milligrams per liter) than the Arrowhead outlet (200 milligrams per liter). Median fecal coliform bacteria event-mean concentrations also were greater at the Meade-Hawthorne outlet site (30,000 colony forming units per 100 milliliters) than the Arrowhead outlet site (17,000 colony forming units per 100 milliliters). A comparison to relevant standards indicates that stormwater runoff from the Downtown drainage basin exceeded criteria for bacteria and TSS, but concentrations generally were below standards for nutrients and metals. Stormwater-quality conditions from the Downtown drainage basin outfalls were similar to or better than stormwater-quality conditions observed in the Arrowhead and Meade-Hawthorne drainage basins. Three wetland channels located at the outlet of the Downtown drainage basin were evaluated for their pollutant reduction capability. Mean reductions in TSS and lead concentrations were greater than 40 percent for all three wetland channels. Total nitrogen, phosphorus, copper, and zinc concentrations also were reduced by at least 20 percent at all three wetlands. Fecal coliform bacteria concentrations typically were reduced by about 21 and 36 percent at the 1st and 2nd Street wetlands, respectively, but the reduction at the 3rd Street wetland channel was nearly zero percent. Total wetland storage volume affected pollutant reductions because TSS, phosphorus, and ammonia reductions were greatest in the wetland with the greatest volume. Chloride concentrations typically increased from inflow to outflow at the 2nd and 3rd Street wetland channels.

Toxic shock syndrome - the seven Rs of management and treatment.

PubMed

Wilkins, Amanda L; Steer, Andrew C; Smeesters, Pierre R; Curtis, Nigel

2017-06-01

Staphylococcal and streptococcal toxic shock syndrome (TSS) are associated with significant morbidity and mortality. There has been considerable progress in understanding the pathophysiology and delineating optimal management and treatment. This article reviews the management of TSS, outlining the 'Seven Rs of Managing and Treating TSS': Recognition, Resuscitation, Removal of source of infection, Rational choice of antibiotics, Role of adjunctive treatment (clindamycin and intravenous immunoglobulin), Review of progress and Reduce risk of secondary cases in close contacts. © 2017 The British Infection Association. Published by Elsevier Ltd. All rights reserved.

40 CFR 471.83 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... Zinc 0.365 0.151 Oil and grease 3.58 3.58 TSS 5.37 4.30 pH (1) (1) 1 Within the range of 7.5 to 10.0 at....17 1.03 Cyanide 0.338 0.135 Zinc 1.73 0.710 Oil and grease 16.9 16.9 TSS 25.4 20.3 pH (1) (1) 1... Cyanide 0.0003 0.0001 Zinc 0.002 0.0006 Oil and grease 0.014 0.014 TSS 0.021 0.017 pH (1) (1) 1 Within the...

40 CFR 471.33 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... Nickel 0.094 0.063 Fluoride 10.1 4.49 Oil and grease 1.70 1.70 TSS 2.55 2.04 pH (1) (1) 1 Within the....103 0.069 Fluoride 11.2 4.94 Oil and grease 1.87 1.87 TSS 2.81 2.25 pH (1) (1) 1 Within the range of 7....122 Nickel 0.450 0.300 Fluoride 48.2 21.4 Oil and grease 8.1 8.1 TSS 12.2 9.72 pH (1) (1) 1 Within the...

40 CFR 471.63 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

....86 TSS 20.0 9.52 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (c) Drawing spent neat... Zinc 0.260 0.109 Ammonia 23.7 10.5 Fluoride 10.6 4.70 Oil and grease 3.56 2.14 TSS 7.30 3.47 pH (1) (1... Zinc 0.146 0.061 Ammonia 13.3 5.86 Fluoride 5.95 2.64 Oil and grease 2.00 1.20 TSS 4.10 1.95 pH (1) (1...

40 CFR 471.63 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

....86 TSS 20.0 9.52 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (c) Drawing spent neat... Zinc 0.260 0.109 Ammonia 23.7 10.5 Fluoride 10.6 4.70 Oil and grease 3.56 2.14 TSS 7.30 3.47 pH (1) (1... Zinc 0.146 0.061 Ammonia 13.3 5.86 Fluoride 5.95 2.64 Oil and grease 2.00 1.20 TSS 4.10 1.95 pH (1) (1...

40 CFR 471.83 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... Zinc 0.365 0.151 Oil and grease 3.58 3.58 TSS 5.37 4.30 pH (1) (1) 1 Within the range of 7.5 to 10.0 at....17 1.03 Cyanide 0.338 0.135 Zinc 1.73 0.710 Oil and grease 16.9 16.9 TSS 25.4 20.3 pH (1) (1) 1... Cyanide 0.0003 0.0001 Zinc 0.002 0.0006 Oil and grease 0.014 0.014 TSS 0.021 0.017 pH (1) (1) 1 Within the...

40 CFR 471.33 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... Nickel 0.094 0.063 Fluoride 10.1 4.49 Oil and grease 1.70 1.70 TSS 2.55 2.04 pH (1) (1) 1 Within the....103 0.069 Fluoride 11.2 4.94 Oil and grease 1.87 1.87 TSS 2.81 2.25 pH (1) (1) 1 Within the range of 7....122 Nickel 0.450 0.300 Fluoride 48.2 21.4 Oil and grease 8.1 8.1 TSS 12.2 9.72 pH (1) (1) 1 Within the...

Cavβ2 transcription start site variants modulate calcium handling in newborn rat cardiomyocytes.

PubMed

Moreno, Cristian; Hermosilla, Tamara; Morales, Danna; Encina, Matías; Torres-Díaz, Leandro; Díaz, Pablo; Sarmiento, Daniela; Simon, Felipe; Varela, Diego

2015-12-01

In the heart, the main pathway for calcium influx is mediated by L-type calcium channels, a multi-subunit complex composed of the pore-forming subunit CaV1.2 and the auxiliary subunits CaVα2δ1 and CaVβ2. To date, five distinct CaVβ2 transcriptional start site (TSS) variants (CaVβ2a-e) varying only in the composition and length of the N-terminal domain have been described, each of them granting distinct biophysical properties to the L-type current. However, the physiological role of these variants in Ca(2+) handling in the native tissue has not been explored. Our results show that four of these variants are present in neonatal rat cardiomyocytes. The contribution of those CaVβ2 TSS variants on endogenous L-type current and Ca(2+) handling was explored by adenoviral-mediated overexpression of each CaVβ2 variant in cultured newborn rat cardiomyocytes. As expected, all CaVβ2 TSS variants increased L-type current density and produced distinctive changes on L-type calcium channel (LTCC) current activation and inactivation kinetics. The characteristics of the induced calcium transients were dependent on the TSS variant overexpressed. Moreover, the amplitude of the calcium transients varied depending on the subunit involved, being higher in cardiomyocytes transduced with CaVβ2a and smaller in CaVβ2d. Interestingly, the contribution of Ca(2+) influx and Ca(2+) release on total calcium transients, as well as the sarcoplasmic calcium content, was found to be TSS-variant-dependent. Remarkably, determination of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) messenger RNA (mRNA) abundance and cell size change indicates that CaVβ2 TSS variants modulate the cardiomyocyte hypertrophic state. In summary, we demonstrate that expression of individual CaVβ2 TSS variants regulates calcium handling in cardiomyocytes and, consequently, has significant repercussion in the development of hypertrophy.

Human T-cell leukemia virus-I tax oncoprotein functionally targets a subnuclear complex involved in cellular DNA damage-response.

PubMed

Haoudi, Abdelali; Daniels, Rodney C; Wong, Eric; Kupfer, Gary; Semmes, O John

2003-09-26

The virally encoded oncoprotein Tax has been implicated in HTLV-1-mediated cellular transformation. The exact mechanism by which this protein contributes to the oncogenic process is not known. However, it has been hypothesized that Tax induces genomic instability via repression of cellular DNA repair. We examined the effect of de novo Tax expression upon the cell cycle, because appropriate activation of cell cycle checkpoints is essential to a robust damage-repair response. Upon induction of tax expression, Jurkat T-cells displayed a pronounced accumulation in G2/M that was reversible by caffeine. We examined the G2-specific checkpoint signaling response in these cells and found activation of the ATM/chk2-mediated pathway, whereas the ATR/chk1-mediated response was unaffected. Immunoprecipitation with anti-chk2 antibody results in co-precipitation of Tax demonstrating a direct interaction of Tax with a chk2-containing complex. We also show that Tax targets a discrete nuclear site and co-localizes with chk2 and not chk1. This nuclear site, previously identified as Tax Speckled Structures (TSS), also contains the early damage response factor 53BP1. The recruitment of 53BP1 to TSS is dependent upon ATM signaling and requires expression of Tax. Specifically, Tax expression induces redistribution of diffuse nuclear 53BP1 to the TSS foci. Taken together these data suggest that the TSS describe a unique nuclear site involved in DNA damage recognition, repair response, and cell cycle checkpoint activation. We suggest that association of Tax with this multifunctional subnuclear site results in disruption of a subset of the site-specific activities and contributes to cellular genomic instability.

40 CFR 426.55 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

...— Metric units (g/kkg of product) TSS 0.70 0.70 Oil 1.40 1 .40 Phosphorus 0.05 .05 pH (1) (1) English units (lb/ton of product) TSS 0.0014 0.0014 Oil 0.0028 .0028 Phosphorus 0.0001 .0001 pH (1) (1) 1 Within the...

40 CFR 426.55 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

...— Metric units (g/kkg of product) TSS 0.70 0.70 Oil 1.40 1 .40 Phosphorus 0.05 .05 pH (1) (1) English units (lb/ton of product) TSS 0.0014 0.0014 Oil 0.0028 .0028 Phosphorus 0.0001 .0001 pH (1) (1) 1 Within the...

40 CFR 417.175 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.01 .05 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.145 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02...

40 CFR 417.185 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.145 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02...

40 CFR 417.195 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.60 0.30 COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20...

40 CFR 417.185 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.175 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.01 .05 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.195 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.60 0.30 COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20...

40 CFR 417.175 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.01 .05 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.185 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.195 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.60 0.30 COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20...

40 CFR 417.175 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.01 .05 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.145 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02...

40 CFR 417.175 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.01 .05 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.14 .07 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.195 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.60 0.30 COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20...

40 CFR 417.195 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.60 0.30 COD 2.70 1.35 TSS 0.20 .10 Surfactants 0.40 .20...

40 CFR 417.135 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... COD 1.50 .75 TSS 0.04 .02 Surfactants 0.30 .15 Oil and grease 0.06 .03 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.30 0.15 COD 1.50 .75 TSS 0.04 .02 Surfactants 0.30 .15...

40 CFR 417.145 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02...

40 CFR 417.145 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.08 .04 TSS 0.06 .03 Surfactants 0.04 .02...

40 CFR 417.185 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01...

40 CFR 417.185 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01 Oil and grease 0.02 .01 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.10 .05 TSS 0.02 .01 Surfactants 0.02 .01...

Organochlorine pesticides in the Johnson Creek Basin, Oregon, 1988-2002

USGS Publications Warehouse

Tanner, Dwight Q.; Lee, Karl K.

2004-01-01

Organochlorine pesticides were detected in unfiltered samples from Johnson Creek that were collected during a storm in March, 2002. Total DDT (the sum of DDT and its metabolites), as well as dieldrin, potentially exceeded Oregon chronic, freshwater criteria at all four Johnson Creek stream-sampling sites. The total DDT criterion was also potentially exceeded at a storm drain at SE 45th Avenue and Umatilla Street. The concentration of total DDT in water samples has decreased by an order of magnitude since previous sampling was done on Johnson Creek in 1989?1990. This decrease was probably due to the movement of these compounds out of the basin and to degradation processes. Concentrations and loads of the organochlorine pesticides were largest at the most upstream sampling site, Johnson Creek at Palmblad Road, which has historically been primarily affected by agricultural land cover. Concentrations and loads were smaller at downstream locations, and there were only a few detections from storm drains. For the purposes of assessing trends in total DDT concentration in Johnson Creek, data for total suspended solids (TSS) were examined, because TSS is often correlated with DDT concentrations, and TSS data are collected routinely by regulatory agencies. As an intermediate step, linear regression was used to relate TSS (measured in the recent study) and turbidity (measured both in the earlier and in the recent studies). For 77 samples, TSS (in mg/L [milligrams per liter]) = 0.88 x Turbidity (in nephleometric turbidity units). The r2 value was 0.82. The TSS concentration (measured, or estimated by the regression) was compared to the concentration of total DDT using linear regression. The TSS concentration associated with meeting the Oregon water-quality criterion for total DDT was 15 to 18 mg/L in the lower and middle part of the basin and 8 mg/L in the upper reaches of the basin. This TSS/DDT relationship is based on only one storm and may not be valid for other conditions of streamflow and runoff. Dieldrin concentration was not well correlated with TSS. Organochlorine compounds also were detected in significant concentrations in Kelley Creek, an important tributary to Johnson Creek, but quality-control considerations made it difficult to interpret some of the data. It does appear, however, that some of the metabolites of DDT were positively associated with TSS. The high concentrations of the DDT metabolites and dieldrin were correlated with agricultural areas.

Biogeochemical cycle of Mercury in an urban stream in Hartford CT

NASA Astrophysics Data System (ADS)

Aragon-jose, A. T.; Bushey, J. T.; Perkins, C.; Mendes, M.; Ulatowski, G.

2012-12-01

Mercury (Hg) toxicity and the potential for bioaccumulation in the food chain result in exposure risk even at low Hg levels. The presence of urban activities can substantially alter Hg fate and transport mechanisms and Hg biogeochemical cycles. Urban watersheds are characterized by high imperviousness and some may even be impacted by combined sewer overflows, both being fundamental factors contributing to Hg loading, mobilization, and shifts in bioavailability in urban watersheds. Research is still needed to characterize the fate and dynamics of Hg in urban streams. To address this gap in knowledge, we collected and characterized stream water and suspended sediment samples in the Park River watershed in Hartford, CT (USA) during baseflow and precipitation events. Sampling sites were selected across an urbanization gradient. Water samples are analyzed for total, dissolved, and particulate Hg and methyl Hg (MeHg), major ions (Cl-, NO3-, SO42-)-, total suspended solids (TSS), and dissolved organic carbon (DOC). Our results show that both total and dissolved Hg concentrations increase in the streams during precipitation events, however, the greatest portion of Hg is associated, and consequently transported, with suspended sediments, as suggested by the high correlation coefficient (R2 ~ 0.80) between TSS and total Hg. No significant correlation was observed between dissolved or total Hg and DOC, contrary to the observations in forested systems, which indicates that the sources and mechanisms governing mobilization and transport of dissolved Hg in an urban watershed differ from those at forested systems. However, during select events, a significant portion of Hg flux occurs in the dissolved phase. Unfiltered MeHg samples exhibited a similar pattern relative to the hydrograph to that of total Hg. Concentrations increase during the rising limb with TSS followed by a decrease as the storm progresses. Dissolved MeHg is mostly below our detection limit. Area normalized THg flux is generally higher at the more developed sites for all but the May storm, whereas the opposite trend is observed for MeHg except for the August storm, indicative of different sources of Hg contributing to the stream. To assist in elucidating the potential sources, dissolved organic matter in the water samples was analyzed for specific ultra violet absorbance at 254 nm (SUVA254) and for excitation-emission matrix (EEMs) to assess differences in organic matter loading to the stream. Additionally, Hg association with sediment was analyzed by collecting four sets of suspended sediment samples over 3-month periods at five sites across the watershed to assess potential sediment sources into the stream. Solid samples were analyzed for total carbon, nitrogen, and hydrogen, organic and inorganic carbon, mercury, acid volatile sulfide, chromium reducible sulfide, PAHs, QACs, and select metals.

Characterization and first flush analysis in road and roof runoff in Shenyang, China.

PubMed

Li, Chunlin; Liu, Miao; Hu, Yuanman; Gong, Jiping; Sun, Fengyun; Xu, Yanyan

2014-01-01

As urbanization increases, urban runoff is an increasingly important component of total urban non-point source pollution. In this study, the properties of urban runoff were examined in Shenyang, in northeastern China. Runoff samples from a tiled roof, a concrete roof and a main road were analyzed for key pollutants (total suspended solids (TSS), total nitrogen (TN), total phosphorus (TP), chemical oxygen demand (COD), Pb, Cd, Cr, Cu, Ni, and Zn). The event mean concentration, site mean concentration, M(V) curves (dimensionless cumulative curve of pollutant load with runoff volume), and mass first flush ratio (MFF30) were used to analyze the characteristics of pollutant discharge and first flush (FF) effect. For all events, the pollutant concentration peaks occurred in the first half-hour after the runoff appeared and preceded the flow peaks. TN is the main pollutant in roof runoff. TSS, TN, TP, Pb, and Cr are the main pollutants in road runoff in Shenyang. There was a significant correlation between TSS and other pollutants except TN in runoff, which illustrated that TSS was an important carrier of organic matter and heavy metals. TN had strong positive correlations with total rainfall (Pearson's r = 0.927), average rainfall (Pearson's r = 0.995), and maximum rainfall intensity (Pearson's r = 0.991). TP had a strong correlation with rainfall intensity (Pearson's r = 0.940). A significant positive correlation between COD and rainfall duration (Pearson's r = 0.902, significance level = 0.05) was found. The order of FF intensity in different surfaces was concrete roof > tile roof > road. Rainfall duration and the length of the antecedent dry period were positively correlated with the FF. TN tended to exhibit strong flush for some events. Heavy metals showed a substantially stronger FF than other pollutant.

The Evaluation and Management of Toxic Shock Syndrome in the Emergency Department: A Review of the Literature.

PubMed

Gottlieb, Michael; Long, Brit; Koyfman, Alex

2018-01-20

Toxic shock syndrome (TSS) is a severe, toxin-mediated illness that can mimic several other diseases and is lethal if not recognized and treated appropriately. This review provides an emergency medicine evidence-based summary of the current evaluation and treatment of TSS. The most common etiologic agents are Staphylococcus aureus and Streptococcus pyogenes. Sources of TSS include postsurgical wounds, postpartum, postabortion, burns, soft tissue injuries, pharyngitis, and focal infections. Symptoms are due to toxin production and infection focus. Early symptoms include fever, chills, malaise, rash, vomiting, diarrhea, and hypotension. Diffuse erythema and desquamation may occur later in the disease course. Laboratory assessment may demonstrate anemia, thrombocytopenia, elevated liver enzymes, and abnormal coagulation studies. Diagnostic criteria are available to facilitate the diagnosis, but they should not be relied on for definitive diagnosis. Rather, specific situations should trigger consideration of this disease process. Treatment involves intravenous fluids, source control, and antibiotics. Antibiotics should include a penicillinase-resistant penicillin, cephalosporin, or vancomycin (in methicillin-resistant S. aureus prevalent areas) along with either clindamycin or linezolid. TSS is a potentially deadly disease requiring prompt recognition and treatment. Focused history, physical examination, and laboratory testing are important for the diagnosis and management of this disease. Understanding the evaluation and treatment of TSS can assist providers with effectively managing these patients. Published by Elsevier Inc.

40 CFR 426.75 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... not exceed— Metric units (q/sq m of product) TSS 0.88 0.88 Oil 1.76 1.76 Phosphorus 0.30 .30 pH (1) (1) English units (lb/1,000 lb of product) TSS 0.18 0.18 Oil 0.36 .36 Phosphorus 0.06 .06 pH (1) (1) 1 Within...

40 CFR 426.75 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... not exceed— Metric units (q/sq m of product) TSS 0.88 0.88 Oil 1.76 1.76 Phosphorus 0.30 .30 pH (1) (1) English units (lb/1,000 lb of product) TSS 0.18 0.18 Oil 0.36 .36 Phosphorus 0.06 .06 pH (1) (1) 1 Within...

40 CFR 466.13 - New source performance standards.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Oil and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units....87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within...

40 CFR 466.13 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Oil and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units....87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within...

40 CFR 466.13 - New source performance standards.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Oil and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units....87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within...

40 CFR 424.15 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/Mwh) TSS 0.024 0.012 Chromium total .0008 .0004 Chromium VI .00008 .00004 Manganese total .008 .0039 pH (1) (1) English units (lb/Mwh) TSS .052 .026 Chromium total .0017 .0009 Chromium VI .0002 .0001 Manganese total .017 .0086 pH (1) (1) 1...

40 CFR 424.15 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/Mwh) TSS 0.024 0.012 Chromium total .0008 .0004 Chromium VI .00008 .00004 Manganese total .008 .0039 pH (1) (1) English units (lb/Mwh) TSS .052 .026 Chromium total .0017 .0009 Chromium VI .0002 .0001 Manganese total .017 .0086 pH (1) (1) 1...

40 CFR 424.15 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/Mwh) TSS 0.024 0.012 Chromium total .0008 .0004 Chromium VI .00008 .00004 Manganese total .008 .0039 pH (1) (1) English units (lb/Mwh) TSS .052 .026 Chromium total .0017 .0009 Chromium VI .0002 .0001 Manganese total .017 .0086 pH (1) (1) 1...

40 CFR 432.125 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... BOD5 26 16 Fecal Coliform (2) (3) O&G (as HEM) 14 8.0 TSS 30 20 1 mg/L (ppm). 2 Maximum of 400 MPN or... Ammonia (as N) 8.0 4.0 BOD5 26 16 Fecal Coliform (2) (3) O&G (as HEM) 14 8.0 TSS 30 20 Total Nitrogen 147...

40 CFR 432.115 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... Coliform ( 2 ) ( 3 ) O&G (as HEM) 14 8.0 TSS 30 20 1 mg/L (ppm). 2 Maximum of 400 MPN or CFU per 100 mL at... Coliform (2) (3) O&G (as HEM) 14 8.0 TSS 30 20 Total Nitrogen 147 103 1 mg/L (ppm). 2 Maximum of 400 MPN or...

40 CFR 432.125 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... BOD5 26 16 Fecal Coliform (2) (3) O&G (as HEM) 14 8.0 TSS 30 20 1 mg/L (ppm). 2 Maximum of 400 MPN or... Ammonia (as N) 8.0 4.0 BOD5 26 16 Fecal Coliform (2) (3) O&G (as HEM) 14 8.0 TSS 30 20 Total Nitrogen 147...

40 CFR 432.115 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... Coliform ( 2 ) ( 3 ) O&G (as HEM) 14 8.0 TSS 30 20 1 mg/L (ppm). 2 Maximum of 400 MPN or CFU per 100 mL at... Coliform (2) (3) O&G (as HEM) 14 8.0 TSS 30 20 Total Nitrogen 147 103 1 mg/L (ppm). 2 Maximum of 400 MPN or...

40 CFR 417.156 - Pretreatment standards for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

...: Pollutant or pollutant property Pretreatment standard BOD5 No limitations. COD Do. TSS Do. Surfactants Do... No limitations. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. (3) For fast turnaround.... Surfactants Do. Oil and grease Do. pH Do. [40 FR 27454, June 30, 1975, as amended at 60 FR 33955, June 29...

40 CFR 417.156 - Pretreatment standards for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

...: Pollutant or pollutant property Pretreatment standard BOD5 No limitations. COD Do. TSS Do. Surfactants Do... No limitations. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. (3) For fast turnaround.... Surfactants Do. Oil and grease Do. pH Do. [40 FR 27454, June 30, 1975, as amended at 60 FR 33955, June 29...

40 CFR 417.156 - Pretreatment standards for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

...: Pollutant or pollutant property Pretreatment standard BOD5 No limitations. COD Do. TSS Do. Surfactants Do... No limitations. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. (3) For fast turnaround.... Surfactants Do. Oil and grease Do. pH Do. [40 FR 27454, June 30, 1975, as amended at 60 FR 33955, June 29...

40 CFR 417.156 - Pretreatment standards for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

...: Pollutant or pollutant property Pretreatment standard BOD5 No limitations. COD Do. TSS Do. Surfactants Do... No limitations. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. (3) For fast turnaround.... Surfactants Do. Oil and grease Do. pH Do. [40 FR 27454, June 30, 1975, as amended at 60 FR 33955, June 29...

A Systematic Review of the Time Series Studies Addressing the Endemic Risk of Acute Gastroenteritis According to Drinking Water Operation Conditions in Urban Areas of Developed Countries

PubMed Central

Beaudeau, Pascal

2018-01-01

Time series studies (TSS) can be viewed as an inexpensive way to tackle the non-epidemic health risk from fecal pathogens in tap water in urban areas. Following the PRISMA recommendations, I reviewed TSS addressing the endemic risk of acute gastroenteritis risk according to drinking water operation conditions in urban areas of developed countries. Eighteen studies were included, covering 17 urban sites (seven in North-America and 10 in Europe) with study populations ranging from 50,000 to 9 million people. Most studies used general practitioner consultations or visits to hospitals for acute gastroenteritis (AGE) as health outcomes. In 11 of the 17 sites, a significant and plausible association was found between turbidity (or particle count) in finished water and the AGE indicator. When provided and significant, the interquartile excess of relative risk estimates ranged from 3–13%. When examined, water temperature, river flow, and produced flow were strongly associated with the AGE indicator. The potential of TSS for the study of the health risk from fecal pathogens in tap water is limited by the lack of specificity of turbidity and its site-sensitive value as an exposure proxy. Nevertheless, at the DWS level, TSS could help water operators to identify operational conditions most at risk, almost if considering other water operation indicators, in addition to turbidity, as possible relevant proxies for exposure. PMID:29701701

Traits of surface water pollution under climate and land use changes: A remote sensing and hydrological modeling approach

NASA Astrophysics Data System (ADS)

Jordan, Yuyan C.; Ghulam, Abduwasit; Hartling, Sean

2014-01-01

In this paper, spatial and temporal trajectories of land cover/land use change (LCLUC) derived from Landsat data record are combined with hydrological modeling to explore the implication of vegetation dynamics on soil erosion and total suspended sediment (TSS) loading to surface rivers. The inter-annual coefficient of variation (CoV) of normalized difference vegetation index (NDVI) is used to screen the LCLUC and climate change. The Soil and Water Assessment Tool (SWAT) is employed to identify the monthly TSS for two times interval (1991 to 2001 and 2001 to 2011) at subbasin levels. SWAT model is calibrated from 1991 to 2001 and validated from 2002 to 2011 at three USGS gauging sites located in the study area. The Spearman's rank correlation of annual mean TSS is used to assess the temporal trends of TSS dynamics in the subbasins in the two study periods. The spatial correlation among NDVI, LCLUC, climate change and TSS loading rate changes is quantified by using linear regression model and negative/positive trend analysis. Our results showed that higher rainfall yields contribute to higher TSS loading into surface waters. A higher inter-annual accumulated vegetation index and lower inter-annual CoV distributed over the uplands resulted in a lower TSS loading rate, while a relatively low vegetation index with larger CoV observed over lowlands resulted in a higher TSS loading rate. The TSS loading rate at the basin outlet increased with the decrease of annual NDVI due to expanding urban areas in the watershed. The results also suggested nonlinearity between the trends of TSS loading with any of a specific land cover change because of the fact that the contribution of a factor can be influenced by the effects of other factors. However, dominant factors that shape the relationship between the trend of TSS loading and specific land cover changes were detected. The change of forest showed a negative relationship while agriculture and pasture demonstrated positive relationships with TSS loading change. Our results do not show any significant causal relationship between urbanization and the TSS loading change suggesting that further investigation needs to be carried out to understand the mechanism of the impact of urban sprawl on surface water quality.

Overview of Research Transition Products

NASA Technical Reports Server (NTRS)

Robinson, John

2014-01-01

Demonstrate increased, more consistent use of Performance- Based Navigation (PBN). Accelerate transfer of NASA scheduling and spacing technologies for inclusion in late mid-term NAS. During high-fidelity human-in-the-loop simulations of Terminal Sequencing and Spacing, air traffic controllers have significantly improved their use of PBN procedures during busy traffic periods without increased workload. Executed an aggressive, short timeframe development schedule. Developed TSS prototype based upon FAA operational systems. Conducted multiple joint FAA/NASA human-in-the-loop simulations. Performed repeated incremental deliveries of tech transfer material to non-traditional RTT stakeholders. Will continue to participate in later phases of FAA acquisition process. ATD-1 transferred Terminal Sequencing and Spacing (TSS) technologies to the FAA. TSS enables routine use of underutilized advanced avionics and PBN procedures. Potential benefits to airlines operating at initial TSS sites estimated to be $300-400M/year. FAA is planning for an initial capability in the NAS in 2018.

Evaluation of Streamflow, Water Quality, and Permitted and Nonpermitted Loads and Yields in the Raritan River Basin, New Jersey, Water Years 1991-98

USGS Publications Warehouse

Reiser, Robert G.

2003-01-01

Seventeen water-quality constituents were analyzed in samples collected from 21 surface-water sampling sites in the Raritan River Basin during water years 1991-97. Loads were computed for seven constituents. Thirteen constituents have associated instream water-quality standards that are used as reference levels when evaluating the data. Nine of the 13 constituents did not meet water-quality reference levels in all samples at all sites. The constituents that most commonly failed to meet the water-quality reference levels in the 801 samples analyzed were total phosphorus (greater than 0.1 mg/L (milligrams per liter) in 32 percent of samples), fecal coliform bacteria (greater than 400 counts/100 milliliters in 29 percent), hardness (less than 50 mg/L in 21 percent), pH (greater than 8.5 or less than 6.5 in 17 percent), and water temperature in designated trout waters (greater than 20 degrees Celsius in 12 percent of samples). Concentrations of chloride, total dissolved solids, nitrate plus nitrite, and sulfate did not exceed water-quality reference levels in any sample. Results from previous studies on pesticides and volatile organic compounds in streamwater during 1996-98, and organic compounds and trace elements in sediments during 1976-93, were summarized for this study. Concentrations of pesticides in some samples exceeded the relevant standards. Water-quality data varied significantly as season and streamflow changed. Concentrations or values of 12 constituents were significantly higher in the growing season than in the nongrowing season at 1 to 21 sites, and concentrations of 6 constituents were significantly higher in the nongrowing season at 1 to 21 sites. Concentrations or values of seven constituents decreased significantly with increased streamflow, indicating a more significant contribution from base flow or permitted sources than from runoff. Concentrations or values of four constituents increased with increased flow, indicating a more significant contribution from runoff than from base flow or permitted sources. Phosphorus concentrations increased with flow at two sites with no point sources and decreased with flow at five sites with four or more permitted point sources. Concentrations of five constituents did not vary significantly with changes in streamflow at any of the sites. Concentrations of constituents differed significantly between sites. The sites with the most desirable values for the most constituents were Mulhockaway Creek, Spruce Run, Millstone River at Manalapan, Manalapan Brook, and Lamington River at Pottersville. The sites with the least desirable values for the most constituents were Millstone River at Blackwells Mills, Matchaponix Brook, Raritan River at Bound Brook, Neshanic River, and Millstone River at Grovers Mill. The total instream loads of seven constituents - total ammonia plus organic nitrogen (TKN), biochemical oxygen demand (BOD), total dissolved solids (TDS), nitrate plus nitrite (NO3+NO2), total organic carbon (TOC), total phosphorus, and total suspended solids (TSS) - were analyzed at low, median, and high flows. The quantities of total instream load that originated from facilities with permits issued by the New Jersey Department of Environmental Protection to discharge effluent to streams (permitted sources) and from other sources (nonpermitted sources) were estimated for each sampling site. TOC and TSS loads primarily were contributed by nonpermitted sources at all flows. BOD and TDS loads primarily were contributed by nonpermitted sources at median and high flows. At low flow, permitted sources contributed more than one-third of the TDS load at 10 sites and more than one-third of the BOD load at 3 sites. Permitted sources contributed more than one-third of the total phosphorus load at 15 and 14 sites at low and median flows, respectively. Permitted sources accounted for more than one-third of total instream load of NO3+NO2 at low- and median-flow conditions at nearly

Loads of nitrate, phosphorus, and total suspended solids from Indiana watersheds

USGS Publications Warehouse

Bunch, Aubrey R.

2016-01-01

Transport of excess nutrients and total suspended solids (TSS) such as sediment by freshwater systems has led to degradation of aquatic ecosystems around the world. Nutrient and TSS loads from Midwestern states to the Mississippi River are a major contributor to the Gulf of Mexico Hypoxic Zone, an area of very low dissolved oxygen concentration in the Gulf of Mexico. To better understand Indiana’s contribution of nutrients and TSS to the Mississippi River, annual loads of nitrate plus nitrite as nitrogen, total phosphorus, and TSS were calculated for nine selected watersheds in Indiana using the load estimation model, S-LOADEST. Discrete water-quality samples collected monthly by the Indiana Department of Environmental Management’s Fixed Stations Monitoring Program from 2000–2010 and concurrent discharge data from the U. S. Geological Survey streamflow gages were used to create load models. Annual nutrient and TSS loads varied across Indiana by watershed and hydrologic condition. Understanding the loads from large river sites in Indiana is important for assessing contributions of nutrients and TSS to the Mississippi River Basin and in determining the effectiveness of best management practices in the state. Additionally, evaluation of loads from smaller upstream watersheds is important to characterize improvements at the local level and to identify priorities for reduction.

40 CFR 426.126 - Pretreatment standards for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... that animal and vegetable oils can be adequately removed in a publicly owned treatment works, whereas... vegetable) (1) (1) Oil (mineral) 230.0 115.0 TSS (1) (1) pH (1) (1) English units (lb/1,000 lb of furnace pull) Oil (animal and vegetable) (1) (1) Oil (mineral) 0.23 0.115 TSS (1) (1) pH (1) (1) Metric units...

40 CFR 426.126 - Pretreatment standards for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... that animal and vegetable oils can be adequately removed in a publicly owned treatment works, whereas... vegetable) (1) (1) Oil (mineral) 230.0 115.0 TSS (1) (1) pH (1) (1) English units (lb/1,000 lb of furnace pull) Oil (animal and vegetable) (1) (1) Oil (mineral) 0.23 0.115 TSS (1) (1) pH (1) (1) Metric units...

Specific insertions of zinc finger domains into Gag-Pol yield engineered retroviral vectors with selective integration properties

PubMed Central

Lim, Kwang-il; Klimczak, Ryan; Yu, Julie H.; Schaffer, David V.

2010-01-01

Retroviral vectors offer benefits of efficient delivery and stable gene expression; however, their clinical use raises the concerns of insertional mutagenesis and potential oncogenesis due to genomic integration preferences in transcriptional start sites (TSS). We have shifted the integration preferences of retroviral vectors by generating a library of viral variants with a DNA-binding domain inserted at random positions throughout murine leukemia virus Gag-Pol, then selecting for variants that are viable and exhibit altered integration properties. We found seven permissive zinc finger domain (ZFD) insertion sites throughout Gag-Pol, including within p12, reverse transcriptase, and integrase. Comprehensive genome integration analysis showed that several ZFD insertions yielded retroviral vector variants with shifted integration patterns that did not favor TSS. Furthermore, integration site analysis revealed selective integration for numerous mutants. For example, two retroviral variants with a given ZFD at appropriate positions in Gag-Pol strikingly integrated primarily into four common sites out of 3.1 × 109 possible human genome locations (P = 4.6 × 10-29). Our findings demonstrate that insertion of DNA-binding motifs into multiple locations in Gag-Pol can make considerable progress toward engineering safer retroviral vectors that integrate into a significantly narrowed pool of sites on human genome and overcome the preference for TSS. PMID:20616052

A unique H2A histone variant occupies the transcriptional start site of active genes.

PubMed

Soboleva, Tatiana A; Nekrasov, Maxim; Pahwa, Anuj; Williams, Rohan; Huttley, Gavin A; Tremethick, David J

2011-12-04

Transcriptional activation is controlled by chromatin, which needs to be unfolded and remodeled to ensure access to the transcription start site (TSS). However, the mechanisms that yield such an 'open' chromatin structure, and how these processes are coordinately regulated during differentiation, are poorly understood. We identify the mouse (Mus musculus) H2A histone variant H2A.Lap1 as a previously undescribed component of the TSS of active genes expressed during specific stages of spermatogenesis. This unique chromatin landscape also includes a second histone variant, H2A.Z. In the later stages of round spermatid development, H2A.Lap1 dynamically loads onto the inactive X chromosome, enabling the transcriptional activation of previously repressed genes. Mechanistically, we show that H2A.Lap1 imparts unique unfolding properties to chromatin. We therefore propose that H2A.Lap1 coordinately regulates gene expression by directly opening the chromatin structure of the TSS at genes regulated during spermatogenesis.

40 CFR 420.134 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... Source Performance Standards (NSPS) Pollutant Maximum daily 1 Maximum monthly avg. 1 TSS 0.00998 0.00465... operations. Subpart M—New Source Performance Standards (NSPS) Pollutant Maximum daily 1 Maximum monthly avg...

40 CFR 420.134 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... Source Performance Standards (NSPS) Pollutant Maximum daily 1 Maximum monthly avg. 1 TSS 0.00998 0.00465... operations. Subpart M—New Source Performance Standards (NSPS) Pollutant Maximum daily 1 Maximum monthly avg...

40 CFR 466.43 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

....55 10.60 0.79 Oil and grease 168.23 12.60 168.23 12.60 TSS 252.35 18.91 201.88 15.12 pH (1) (1) (1....32 Iron 4.24 0.32 2.17 0.16 Oil and grease 34.46 2.58 34.46 2.58 TSS 51.69 3.87 41.35 3.10 pH (1) (1...

Comparative impacts of stormwater runoff on water quality of an urban, a suburban, and a rural stream.

PubMed

Mallin, Michael A; Johnson, Virginia L; Ensign, Scott H

2009-12-01

Water quality data at 12 sites within an urban, a suburban, and a rural stream were collected contemporaneously during four wet and eight dry periods. The urban stream yielded the highest biochemical oxygen demand (BOD), orthophosphate, total suspended sediment (TSS), and surfactant concentrations, while the most rural stream yielded the highest total organic carbon concentrations. Percent watershed development and percent impervious surface coverage were strongly correlated with BOD (biochemical oxygen demand), orthophosphate, and surfactant concentrations but negatively with total organic carbon. Excessive fecal coliform abundance most frequently occurred in the most urbanized catchments. Fecal coliform bacteria, TSS, turbidity, orthophosphate, total phosphorus, and BOD were significantly higher during rain events compared to nonrain periods. Total rainfall preceding sampling was positively correlated with turbidity, TSS, BOD, total phosphorus, and fecal coliform bacteria concentrations. Turbidity and TSS were positively correlated with phosphorus, fecal coliform bacteria, BOD, and chlorophyll a, which argues for better sedimentation controls under all landscape types.

40 CFR 417.152 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE... COD 0.15 .05 TSS 0.03 .01 Surfactants 0.06 .02 Oil and grease 0.015 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.03 0.01 COD 0.15 .05 TSS 0.03 .01 Surfactants 0.06 .02...

40 CFR 417.152 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE... COD 0.15 .05 TSS 0.03 .01 Surfactants 0.06 .02 Oil and grease 0.015 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.03 0.01 COD 0.15 .05 TSS 0.03 .01 Surfactants 0.06 .02...

40 CFR 420.104 - New source performance standards (NSPS).

Code of Federal Regulations, 2014 CFR

2014-07-01

... TSS 0.00125 0.000626 O&G 0.000522 0.000209 Chromium 1 0.0000209 0.0000084 Lead 0.0000094 0.0000031... pH (2) (2) 1 The limitations for chromium and nickel shall be applicable in lieu of those for lead... consecutive days Kg/kkg (pounds per 1,000 lb) of product TSS 0.00250 0.00125 O&G 0.00104 0.000417 Chromium 1 0...

40 CFR 420.104 - New source performance standards (NSPS).

Code of Federal Regulations, 2012 CFR

2012-07-01

... TSS 0.00125 0.000626 O&G 0.000522 0.000209 Chromium 1 0.0000209 0.0000084 Lead 0.0000094 0.0000031... pH (2) (2) 1 The limitations for chromium and nickel shall be applicable in lieu of those for lead... consecutive days Kg/kkg (pounds per 1,000 lb) of product TSS 0.00250 0.00125 O&G 0.00104 0.000417 Chromium 1 0...

40 CFR 420.104 - New source performance standards (NSPS).

Code of Federal Regulations, 2013 CFR

2013-07-01

... TSS 0.00125 0.000626 O&G 0.000522 0.000209 Chromium 1 0.0000209 0.0000084 Lead 0.0000094 0.0000031... pH (2) (2) 1 The limitations for chromium and nickel shall be applicable in lieu of those for lead... consecutive days Kg/kkg (pounds per 1,000 lb) of product TSS 0.00250 0.00125 O&G 0.00104 0.000417 Chromium 1 0...

40 CFR 411.25 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (maximum for any 1 day) Metric units (kg/kkg of dust leached) TSS 0.4. Temperature (heat) Not to exceed 3 °C rise above inlet temperature. pH Within the range 6.0 to 9.0. English units (lb/1,000 lb of dust leached) TSS 0.4. Temperature (heat) Not to exceed 3 °C rise above inlet temperature. pH Within the range...

40 CFR 411.25 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (maximum for any 1 day) Metric units (kg/kkg of dust leached) TSS 0.4. Temperature (heat) Not to exceed 3 °C rise above inlet temperature. pH Within the range 6.0 to 9.0. English units (lb/1,000 lb of dust leached) TSS 0.4. Temperature (heat) Not to exceed 3 °C rise above inlet temperature. pH Within the range...

40 CFR 420.104 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... TSS 0.00125 0.000626 O&G 0.000522 0.000209 Chromium 1 0.0000209 0.0000084 Lead 0.0000094 0.0000031... pH (2) (2) 1 The limitations for chromium and nickel shall be applicable in lieu of those for lead... consecutive days Kg/kkg (pounds per 1,000 lb) of product TSS 0.00250 0.00125 O&G 0.00104 0.000417 Chromium 1 0...

40 CFR 420.104 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... TSS 0.00125 0.000626 O&G 0.000522 0.000209 Chromium 1 0.0000209 0.0000084 Lead 0.0000094 0.0000031... pH (2) (2) 1 The limitations for chromium and nickel shall be applicable in lieu of those for lead... consecutive days Kg/kkg (pounds per 1,000 lb) of product TSS 0.00250 0.00125 O&G 0.00104 0.000417 Chromium 1 0...

40 CFR 455.25 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

...(d), and subject to 455.20(a), must meet the following standards for BOD5, TSS, COD and pH: New... of daily values shall not exceed** COD 9.360 6.480 BOD5 5.328 1.1520 TSS 4.392 1.2960 pH * * * Within... concentrations listed in table 4 or table 5 of this part, as appropriate, of this subpart. [58 FR 50690, Sept. 28...

40 CFR 455.25 - New source performance standards (NSPS).

Code of Federal Regulations, 2013 CFR

2013-07-01

... CFR 455.20(d), and subject to 455.20(a), must meet the following standards for BOD5, TSS, COD and pH...** Average of daily values shall not exceed** COD 9.360 6.480 BOD5 5.328 1.1520 TSS 4.392 1.2960 pH... concentrations listed in table 4 or table 5 of this part, as appropriate, of this subpart. [58 FR 50690, Sept. 28...

40 CFR 455.25 - New source performance standards (NSPS).

Code of Federal Regulations, 2014 CFR

2014-07-01

... CFR 455.20(d), and subject to 455.20(a), must meet the following standards for BOD5, TSS, COD and pH...** Average of daily values shall not exceed** COD 9.360 6.480 BOD5 5.328 1.1520 TSS 4.392 1.2960 pH... concentrations listed in table 4 or table 5 of this part, as appropriate, of this subpart. [58 FR 50690, Sept. 28...

Prevalence of toxic shock syndrome toxin 1 (TSST-1)-producing strains of Staphylococcus aureus and antibody to TSST-1 among healthy Japanese women.

PubMed

Parsonnet, Jeffrey; Goering, Richard V; Hansmann, Melanie A; Jones, Michaelle B; Ohtagaki, Kumiko; Davis, Catherine C; Totsuka, Kyoichi

2008-08-01

Many cases of neonatal toxic shock syndrome (TSS)-like exanthematous disease but few cases of menstrual TSS (mTSS) have been reported in Japan. We determined the prevalence of mucosal colonization with Staphylococcus aureus and of positive antibodies to TSS toxin 1 (TSST-1) among 209 healthy Japanese women in Tokyo. S. aureus isolates from mucosal sites were characterized with respect to TSST-1 production and resistance genotype. Antibody titers were determined for test subjects and for 133 Japanese and 137 Caucasian control women living in the United States. S. aureus was isolated from at least one site in 108 of 209 women (52%) in Tokyo. Of the 159 S. aureus isolates recovered, 14 (9%) were TSST-1 positive (12 unique strains). Twelve of 209 women (6%) were colonized with a TSST-1-producing strain; two (<1%) had vaginal colonization. Only 2 of 12 unique toxigenic strains (14%) were methicillin resistant. Of the 12 TSST-1-positive strains isolated, 6 (50%) were pulsed-field gel electrophoresis type USA200, multilocus sequence type clonal complex 30. Fewer Japanese women in Tokyo (47%) than Caucasian and Japanese women in the United States (89% and 75%, respectively) had TSST-1 antibodies. The prevalences of colonization with TSST-1-producing S. aureus were comparable in Japan and the United States, despite low seropositivity to TSST-1 in Japan. Environmental factors appear to be important in promoting the development of anti-TSST-1 antibodies, as there was a significant difference in titers between Japanese women living in Tokyo and those living in the United States. Most colonizing TSST-1-producing S. aureus strains in Japan were genotypically similar to mTSS strains found in the United States.

NATURAL ATTENUATION OF CHLORINATED SOLVENTS AT MULTIPLE AIR FORCE BASE DEMONSTRATION SITES

EPA Science Inventory

Natural attenuation treatability studies(TSs) were conducted at 14 US Air Force bases. Only sites where biodegradation of CAHs was suspected were selected for the study. The major initiative was to evaluate the effectiveness of monitored natural attenuation(MNA) at sites contam...

DNA context represents transcription regulation of the gene in mouse embryonic stem cells

NASA Astrophysics Data System (ADS)

Ha, Misook; Hong, Soondo

2016-04-01

Understanding gene regulatory information in DNA remains a significant challenge in biomedical research. This study presents a computational approach to infer gene regulatory programs from primary DNA sequences. Using DNA around transcription start sites as attributes, our model predicts gene regulation in the gene. We find that H3K27ac around TSS is an informative descriptor of the transcription program in mouse embryonic stem cells. We build a computational model inferring the cell-type-specific H3K27ac signatures in the DNA around TSS. A comparison of embryonic stem cell and liver cell-specific H3K27ac signatures in DNA shows that the H3K27ac signatures in DNA around TSS efficiently distinguish the cell-type specific H3K27ac peaks and the gene regulation. The arrangement of the H3K27ac signatures inferred from the DNA represents the transcription regulation of the gene in mESC. We show that the DNA around transcription start sites is associated with the gene regulatory program by specific interaction with H3K27ac.

Turbidity and Total Suspended Solids on the Lower Cache River Watershed, AR.

PubMed

Rosado-Berrios, Carlos A; Bouldin, Jennifer L

2016-06-01

The Cache River Watershed (CRW) in Arkansas is part of one of the largest remaining bottomland hardwood forests in the US. Although wetlands are known to improve water quality, the Cache River is listed as impaired due to sedimentation and turbidity. This study measured turbidity and total suspended solids (TSS) in seven sites of the lower CRW; six sites were located on the Bayou DeView tributary of the Cache River. Turbidity and TSS levels ranged from 1.21 to 896 NTU, and 0.17 to 386.33 mg/L respectively and had an increasing trend over the 3-year study. However, a decreasing trend from upstream to downstream in the Bayou DeView tributary was noted. Sediment loading calculated from high precipitation events and mean TSS values indicate that contributions from the Cache River main channel was approximately 6.6 times greater than contributions from Bayou DeView. Land use surrounding this river channel affects water quality as wetlands provide a filter for sediments in the Bayou DeView channel.

DNA context represents transcription regulation of the gene in mouse embryonic stem cells.

PubMed

Ha, Misook; Hong, Soondo

2016-04-14

Understanding gene regulatory information in DNA remains a significant challenge in biomedical research. This study presents a computational approach to infer gene regulatory programs from primary DNA sequences. Using DNA around transcription start sites as attributes, our model predicts gene regulation in the gene. We find that H3K27ac around TSS is an informative descriptor of the transcription program in mouse embryonic stem cells. We build a computational model inferring the cell-type-specific H3K27ac signatures in the DNA around TSS. A comparison of embryonic stem cell and liver cell-specific H3K27ac signatures in DNA shows that the H3K27ac signatures in DNA around TSS efficiently distinguish the cell-type specific H3K27ac peaks and the gene regulation. The arrangement of the H3K27ac signatures inferred from the DNA represents the transcription regulation of the gene in mESC. We show that the DNA around transcription start sites is associated with the gene regulatory program by specific interaction with H3K27ac.

Characterization of a spliced variant of human IRF-3 promoter and its regulation by the transcription factor Sp1.

PubMed

Ren, Wei; Zhu, Liang-Hua; Xu, Hua-Guo; Jin, Rui; Zhou, Guo-Ping

2012-06-01

Interferon regulatory factor 3 (IRF-3), an essential transcriptional regulator of the interferon genes, plays an important role in host defense against viral and microbial infection as well as in cell growth regulation. Promoter plays a crucial role in gene transcription. We have reported the characterization of the wide type of human IRF-3 promoter, but the characterization of the spliced variant of human IRF-3 Int2V1 promoter has not been systematically analyzed. To observe the spliced variant of human IRF-3 promoter, we have cloned the human IRF-3 gene promoter region containing 300 nucleotides upstream the transcription start site (TSS). Transient transfection of 5' deleted promoter-reporter constructs and luciferase assay illustrated the region -159/-100 relative to the TSS is sufficient for full promoter activity. This region contains GATA1 and specific protein-1 (Sp1) transcription factor binding sites. Interestingly, mutation of this Sp1 site reduced the promoter activity by 50%. However, overexpression of Sp1 increased the transcription activity by 2.4-fold. These results indicated that the spliced variant of human IRF-3 gene core promoter was located within the region -159/-100 relative to the TSS. Sp1 transcription factor upregulates the spliced variant of human IRF-3 gene promoter.

40 CFR 406.62 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice...— Metric units (kilograms per 1,000 kg of rice) BOD5 0.42 0.14 TSS 0.24 .08 pH (1) (1) English units (pounds per hundredweight of rice) BOD5 0.042 0.014 TSS 0.024 .008 pH (1) (1) 1 Within the range 6.0 to 9...

40 CFR 406.62 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice...— Metric units (kilograms per 1,000 kg of rice) BOD5 0.42 0.14 TSS 0.24 .08 pH (1) (1) English units (pounds per hundredweight of rice) BOD5 0.042 0.014 TSS 0.024 .008 pH (1) (1) 1 Within the range 6.0 to 9...

40 CFR 417.122 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE... COD 4.05 1.35 TSS 0.09 .03 Surfactants 0.90 .30 Oil and grease 0.15 .05 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.90 0.30 COD 4.05 1.35 TSS 0.09 .03 Surfactants 0.90 .30...

40 CFR 417.122 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE... COD 4.05 1.35 TSS 0.09 .03 Surfactants 0.90 .30 Oil and grease 0.15 .05 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.90 0.30 COD 4.05 1.35 TSS 0.09 .03 Surfactants 0.90 .30...

40 CFR 417.122 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE... COD 4.05 1.35 TSS 0.09 .03 Surfactants 0.90 .30 Oil and grease 0.15 .05 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.90 0.30 COD 4.05 1.35 TSS 0.09 .03 Surfactants 0.90 .30...

40 CFR 464.34 - New source performance standards.

Code of Federal Regulations, 2010 CFR

2010-07-01

... scrubbed) effluent standards for copper, lead, zinc, total phenols, oil and grease, and TSS. For non...) 0.0129 0.0071 Lead (T) 0.0237 0.0116 Zinc (T) 0.0437 0.0165 Oil and grease 1.34 0.446 TSS 0.67 0.536... average Annual average 1 (mg/l) 2 (mg/l) 2 Copper (T) 0.29 0.16 0.0029 Lead (T) 0.53 0.26 0.0067 Zinc (T...

40 CFR 464.34 - New source performance standards.

Code of Federal Regulations, 2011 CFR

2011-07-01

... scrubbed) effluent standards for copper, lead, zinc, total phenols, oil and grease, and TSS. For non...) 0.0129 0.0071 Lead (T) 0.0237 0.0116 Zinc (T) 0.0437 0.0165 Oil and grease 1.34 0.446 TSS 0.67 0.536... average Annual average 1 (mg/l) 2 (mg/l) 2 Copper (T) 0.29 0.16 0.0029 Lead (T) 0.53 0.26 0.0067 Zinc (T...

40 CFR 417.155 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... COD 0.08 .04 TSS 0.04 .02 Surfactants 0.04 .02 Oil and grease 0.01 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.08 .04 TSS 0.04 .02 Surfactants 0.04 .02... Surfactants 0.20 .10 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous...

[Staphylococcal toxic shock syndrome after breast surgery].

PubMed

Pelissier, A; Dumesnil, J; Levy, R; Charron, C; Rouzier, R

2014-09-01

The surgical site infection occurs within 30 days after surgery. It is the most common complication of surgery, with a rate of 1 to 5% without antibiotic prophylaxis and less than 1% with antibiotic prophylaxis. The toxic shock syndrome (TSS) is a dramatic complication. We report the case 39-year-old woman who presented a life-threatening TSS acquired after breast surgery. We describe the signs and symptoms of this condition as well as treatment principles. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

Determination of the effect of wind velocity and direction changes on turbidity removal in rectangular sedimentation tanks.

PubMed

Khezri, Seyed Mostafa; Biati, Aida; Erfani, Zeynab

2012-01-01

In the present study, a pilot-scale sedimentation tank was used to determine the effect of wind velocity and direction on the removal efficiency of particles. For this purpose, a 1:20 scale pilot simulated according to Frude law. First, the actual efficiency of total suspended solids (TSS) removal was calculated in no wind condition. Then, the wind was blown in the same and the opposite directions of water flow. At each direction TSS removal was calculated at three different velocities from 2.5 to 7 m/s. Results showed that when the wind was in the opposite direction of water flow, TSS removal efficiency initially increased with the increase of wind velocity from 0 to 2.5 m/s, then it decreased with the increase of velocity to 5 m/s. This mainly might happen because the opposite direction of wind can increase particles' retention time in the sedimentation tank. However, higher wind velocities (i.e. 3.5 and 5.5 m/s) could not increase TSS removal efficiency. Thus, if sedimentation tanks are appropriately exposed to the wind, TSS removal efficiency increases by approximately 6%. Therefore, energy consumption will be reduced by a proper site selection for sedimentation tank unit in water and waste water treatment plants.

40 CFR 406.102 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Wheat Starch and...— Metric units (kg/kkg of raw material (wheat flour)) BOD5 6.0 2.0 TSS 6.0 2.0 pH (1) (1) English units (lb/1,000 lb of raw material (wheat flour)) BOD5 6.0 2.0 TSS 6.0 2.0 pH (1) (1) 1 Within the range 6.0...

40 CFR 406.42 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour...— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.025 0.0083 TSS 0.025 .0083 pH (1) (1) English units (pounds per 1,000 stdbu of wheat) BOD5 1.50 0.50 TSS 1.50 .50 pH (1) (1) 1 Within the range 6.0 to 9.0...

40 CFR 417.165 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.10 0.05 COD 0.44 .22 TSS 0.01 .005 Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1... units (kilograms per 1,000 kg of anhydrous product) BOD5 0.02. COD 0.07. TSS 0.002. Surfactants 0.02...

40 CFR 417.165 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.10 0.05 COD 0.44 .22 TSS 0.01 .005 Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1... units (kilograms per 1,000 kg of anhydrous product) BOD5 0.02. COD 0.07. TSS 0.002. Surfactants 0.02...

40 CFR 417.165 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.10 0.05 COD 0.44 .22 TSS 0.01 .005 Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1... units (kilograms per 1,000 kg of anhydrous product) BOD5 0.02. COD 0.07. TSS 0.002. Surfactants 0.02...

40 CFR 417.165 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.10 0.05 COD 0.44 .22 TSS 0.01 .005 Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1... units (kilograms per 1,000 kg of anhydrous product) BOD5 0.02. COD 0.07. TSS 0.002. Surfactants 0.02...

40 CFR 417.165 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.10 0.05 COD 0.44 .22 TSS 0.01 .005 Surfactants 0.10 .05 Oil and grease 0.01 .005 pH (1) (1... units (kilograms per 1,000 kg of anhydrous product) BOD5 0.02. COD 0.07. TSS 0.002. Surfactants 0.02...

CHD1 regulates cell fate determination by activation of differentiation-induced genes

PubMed Central

Baumgart, Simon J.; Najafova, Zeynab; Hossan, Tareq; Xie, Wanhua; Nagarajan, Sankari; Kari, Vijayalakshmi; Ditzel, Nicholas; Kassem, Moustapha

2017-01-01

Abstract The coordinated temporal and spatial activation of gene expression is essential for proper stem cell differentiation. The Chromodomain Helicase DNA-binding protein 1 (CHD1) is a chromatin remodeler closely associated with transcription and nucleosome turnover downstream of the transcriptional start site (TSS). In this study, we show that CHD1 is required for the induction of osteoblast-specific gene expression, extracellular-matrix mineralization and ectopic bone formation in vivo. Genome-wide occupancy analyses revealed increased CHD1 occupancy around the TSS of differentiation-activated genes. Furthermore, we observed that CHD1-dependent genes are mainly induced during osteoblast differentiation and are characterized by higher levels of CHD1 occupancy around the TSS. Interestingly, CHD1 depletion resulted in increased pausing of RNA Polymerase II (RNAPII) and decreased H2A.Z occupancy close to the TSS, but not at enhancer regions. These findings reveal a novel role for CHD1 during osteoblast differentiation and provide further insights into the intricacies of epigenetic regulatory mechanisms controlling cell fate determination. PMID:28475736

CHD1 regulates cell fate determination by activation of differentiation-induced genes.

PubMed

Baumgart, Simon J; Najafova, Zeynab; Hossan, Tareq; Xie, Wanhua; Nagarajan, Sankari; Kari, Vijayalakshmi; Ditzel, Nicholas; Kassem, Moustapha; Johnsen, Steven A

2017-07-27

The coordinated temporal and spatial activation of gene expression is essential for proper stem cell differentiation. The Chromodomain Helicase DNA-binding protein 1 (CHD1) is a chromatin remodeler closely associated with transcription and nucleosome turnover downstream of the transcriptional start site (TSS). In this study, we show that CHD1 is required for the induction of osteoblast-specific gene expression, extracellular-matrix mineralization and ectopic bone formation in vivo. Genome-wide occupancy analyses revealed increased CHD1 occupancy around the TSS of differentiation-activated genes. Furthermore, we observed that CHD1-dependent genes are mainly induced during osteoblast differentiation and are characterized by higher levels of CHD1 occupancy around the TSS. Interestingly, CHD1 depletion resulted in increased pausing of RNA Polymerase II (RNAPII) and decreased H2A.Z occupancy close to the TSS, but not at enhancer regions. These findings reveal a novel role for CHD1 during osteoblast differentiation and provide further insights into the intricacies of epigenetic regulatory mechanisms controlling cell fate determination. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

40 CFR 415.365 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Copper Salts Production... producing copper sulfate, copper chloride, copper iodide, or copper nitrate must achieve the following new source performance standards (NSPS): The limitations for pH, TSS, copper (T), nickel (T), and selenium (T...

40 CFR 415.365 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Copper Salts Production... producing copper sulfate, copper chloride, copper iodide, or copper nitrate must achieve the following new source performance standards (NSPS): The limitations for pH, TSS, copper (T), nickel (T), and selenium (T...

40 CFR 422.65 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) EFFLUENT GUIDELINES AND STANDARDS PHOSPHATE MANUFACTURING POINT SOURCE CATEGORY Sodium Phosphates... consecutive days shall not exceed— TSS 0.35 0.18 Total phosphorus (as P) .56 .28 Fluoride (as F) .21 .11 pH (1...

40 CFR 422.65 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) EFFLUENT GUIDELINES AND STANDARDS PHOSPHATE MANUFACTURING POINT SOURCE CATEGORY Sodium Phosphates... consecutive days shall not exceed— TSS 0.35 0.18 Total phosphorus (as P) .56 .28 Fluoride (as F) .21 .11 pH (1...

40 CFR 406.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling...— Metric units (kilograms per 1,000 kg of corn) BOD5 0.21 0.07 TSS 0.18 .06 pH (1) (1) English units (pounds per 1,000 stdbu of corn) BOD5 12.0 4.0 TSS 10.5 3.5 pH (1) (1) 1 Within the range 6.0 to 9.0. [39...

40 CFR 406.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling...— Metric units (kilograms per 1,000 kg of corn) BOD5 0.21 0.07 TSS 0.18 .06 pH (1) (1) English units (pounds per 1,000 stdbu of corn) BOD5 12.0 4.0 TSS 10.5 3.5 pH (1) (1) 1 Within the range 6.0 to 9.0. [39...

40 CFR 471.23 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... 4.37 Fluoride 4.44 1.97 Oil and grease 0.746 0.746 TSS 1.12 0.895 pH (1) (1) 1 Within the range of 7... 7.63 Oil and grease 2.89 2.89 TSS 4.34 3.47 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all... 59.3 47.4 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (f) Surface treatment spent...

40 CFR 434.55 - New source performance standards (NSPS).

Code of Federal Regulations, 2013 CFR

2013-07-01

... consecutive days Concentrations in mg/1 Iron, total 6.0 3.0 TSS 70.0 35.0 pH (1) (1) 1 Within the range 6.0 to... property Limitations Settleable Solids 0.5 ml/1 maximum not to be exceeded. pH (1) (1) Within the range 6.0..., total 4.0 2.0 TSS 70.0 35.0 pH (1) (1) 1 Within the range 6.0 to 9.0 at all times. (2) Except as...

[Toxic-shock syndrome. Three cases (author's transl)].

PubMed

Rapin, M; D'Enfert, J; Cabane, J

1981-06-13

Several cases of toxic shock syndrome (T.S.S) have been recently reported from the U.S.A. Clinical features of this new syndrome include fever, desquamative scarlatiniform rash, hypotension and involvement of central nervous system, liver, kidney and muscles. More than 90% of cases are women with staphylococcic vaginitis using tampons during menstruations. A toxin produced by staphylococcus aureus is thought to be the causative agent, because the germ has been isolated in local (vaginal, pharyngeal, subcutaneous or other sites) but not systemic (blood, cerebrospinal fluid) cultures. The mortality rate is 3-10%, and relapse can occur. We report the first three french cases of T.S.S.: a 17 year old girl with typical tampon-associated vaginitis, a 36 year old woman with a postoperative peritonitis and a 20 year old man with a popliteal abscess. Staphylococcus aureus of type I or IV was identified at the site of infection in all cases, but never in blood cultures. These three patients recovered with antistaphylococcic antibiotics and supportive therapy, but local treatment of infections seems to have been of utmost importance. These cases suggest that T.S.S. can occur with several staphylococcus serotypes and confirm that this syndrome is not always associated with tampons and vaginitis.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zeng, Yanli; Li, Hui; Zhang, Xiaoju

Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G, A3G) exert antiviral defense as an important factor of innate immunity. A variety of cytokines such as IFN-γ,IL2,IL15,IL7 could induce the transcription of A3G. However, the regulation of other nuclear factor on the transcription of A3G have not been reported at the present. To gain new insights into the transcriptional regulation of this restriction factor, we cloned and characterized the promoter region of A3G and investigate the modulation of USF1 gene on the transcription of A3G. We identified a 232 bp region that was sufficient to regulate the activity of full promoter. Transcriptionalmore » start sites (TSS) were identified by the luciferase reporter assays of plasmids containing full or shorter fragments of the A3G promoter. The results demonstrated that the core promoter of A3G is located within the region -159/-84 relative to the TSS. Transcriptional activity of A3G core promoter regulated by USF1 was dependent on an E-box (located at position -91/-86 relative to the major TSS) and was abolished after mutation of this DNA element. USF1 gene can take part in basal transcription regulation of the human A3G gene in hepatocyte, and the identified E-box represented a binding site for the USF1. - Highlights: • The core promoter of A3G is located within the region −159/−84 relative to the TSS. • Transcriptional activity of A3G core promoter regulated by USF1 was dependent on an E-box (located at position −91/−86 relative to the major TSS). • USF1 gene can take part in basal transcription regulation of the human A3G gene in hepatocyte.« less

Effects of sub-Arctic shrub canopies on snowmelt energetics

NASA Astrophysics Data System (ADS)

Bewley, D.; Essery, R.; Pomeroy, J.

2006-12-01

Much of the low Arctic is covered with shrub tundra, and there is increasing evidence that snowmelt rates are substantially different between shrub tundra and poorly vegetated sites. The cause of this remains uncertain, however, and extends beyond simple differences in albedo. Results are presented in this study from a detailed field investigation at Wolf Creek Research Basin in 2004 to determine the effect of two different shrub canopy structures on both melt rates and the partitioning of melt energy. The low shrub site (LSS) was essentially an unvegetated snowfield prior to melt (mean albedo ~0.85), and shrubs only became exposed during the last few days of melt reaching a mean height of 0.31 m and mean Plant Area Index (PAI) of 0.32. Shrubs at the tall shrub site (TSS) were partially buried initially (shrub fraction, mean height and PAI of 0.2, 0.9 m and 0.41) but dominated the landscape by the end of melt (corresponding values of 0.71, 1.6 m and 0.6). Melt rates were higher at TSS up until the exposure of shrubs and bare ground at LSS, after which the rates converged. A Shrub-Snow Canopy Model (SSCM) is developed to improve snowmelt simulations for shrub canopies by parameterizing the key shrub effects on surface fluxes, including the extinction of shortwave irradiance beneath shrubs and in canopy gaps, and the enhancement of snow surface fluxes of longwave radiation and sensible heat. SSCM was run for LSS assuming no shrubs were present above the variable snow and bare ground tiles, whereas for TSS an increasing shrub fraction above each tile was prescribed from observations. Results from both sites suggest that sensible heat fluxes contributed more melt energy than net radiation, and were greater during early melt at TSS due to the warming of exposed shrubs. SWE was accurately predicted against transect measurements at TSS (rms error 4 mm), but was overestimated at LSS (rms error 13 mm) since both air temperatures and turbulent transport were underestimated by not incorporating shrubs. This demonstrates the need to incorporate the rapid change in surface conditions associated with any shrub canopy (low or tall) within land surface and hydrological models. Most of the information required for running SSCM at other (tall) shrub canopies can be obtained remotely from photos or images of sufficiently high resolution to delineate individual shrub patches and canopy gaps.

A Satellite Imagery Approach to Monitor Turbidity and Total Suspended Sediments in Green Bay, WI

NASA Astrophysics Data System (ADS)

Khazaei, B.; Hamidi, S.; Hosseiny, S. M. H.; Ekhtari, N.

2017-12-01

Fox River is a major source of land-based pollutants, nutrients, and sediment that flows into the southern Green Bay (GB). GB supplies one-third of the total nutrient loading to Lake Michigan. This can play a significant role in the biological functioning of the Bay and development of managerial scenarios. To name a few, it can degrade the quality of the aquatic life, add to the costs for treatment processes, and reduce coastal quality. Water quality evaluation is a time consuming and costly process. Spaceborne imagery data provides a cheap and valuable source of information as an alternative for field monitoring of the water resources. Sediment is an optically active variable; hence; remote sensing techniques can be utilized to estimate Total Suspended Sediments (TSS) and Turbidity (TU) of water. In this study, we developed relationships between remote sensing imagery data with daily in situ measurements of TSS and TU in the summers of 2011 to 2014. Surface reflectance (SR) values obtained from Band 1 of MYD09GQ dataset-a level 2 product of MODerate Resolution Imaging Spectroradiometer (MODIS). This band covers SR between 620 and 670nm, in which, the wavelength is sensitive to mineral suspended matters most. After elimination of days with cloud contamination, 118 pairs of data remained for analysis. Several possible functions were tested and exponential function was the best estimator of the SR-TSS and SR-TU relationships with R2 values of 0.8269 and 0.8688, respectively. We then used 2014 data to validate the proposed functions. The model was able to estimate TSS and TU with NRMSE values of 0.36 and 0.30. It indicates that the model can be well-applied to predict TSS and TU within a reasonable margin of error. Then, equations were used to map the spatiotemporal dynamics of sediment in GB. Area of the plume ranges between 12 to 180 km2 while 50% of the time the area of the turbid plume is more than 106 km2. Expectedly, the concentration of sediment is much higher near the Fox River mouth. It suggests that the main source of sediment in the southern GB is river discharge, even though other factors such as wind driven wave, gyres, and circulation affect the TSS concentration. The proposed approach can be extended to other coastal regions of the Great Lakes and provides a framework to study pollution transportation in coastal areas.

Folding behavior of a T-shaped, ribosome-binding translation enhancer implicated in a wide-spread conformational switch

PubMed Central

Le, My-Tra; Kasprzak, Wojciech K; Kim, Taejin; Gao, Feng; Young, Megan YL; Yuan, Xuefeng; Shapiro, Bruce A; Seog, Joonil; Simon, Anne E

2017-01-01

Turnip crinkle virus contains a T-shaped, ribosome-binding, translation enhancer (TSS) in its 3’UTR that serves as a hub for interactions throughout the region. The viral RNA-dependent RNA polymerase (RdRp) causes the TSS/surrounding region to undergo a conformational shift postulated to inhibit translation. Using optical tweezers (OT) and steered molecular dynamic simulations (SMD), we found that the unusual stability of pseudoknotted element H4a/Ψ3 required five upstream adenylates, and H4a/Ψ3 was necessary for cooperative association of two other hairpins (H5/H4b) in Mg2+. SMD recapitulated the TSS unfolding order in the absence of Mg2+, showed dependence of the resistance to pulling on the 3D orientation and gave structural insights into the measured contour lengths of the TSS structure elements. Adenylate mutations eliminated one-site RdRp binding to the 3’UTR, suggesting that RdRp binding to the adenylates disrupts H4a/Ψ3, leading to loss of H5/H4b interaction and promoting a conformational switch interrupting translation and promoting replication. DOI: http://dx.doi.org/10.7554/eLife.22883.001 PMID:28186489

40 CFR 415.65 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

...) Any new source subject to this subpart and using the mercury cell process must achieve the following new source performance standards (NSPS): Subpart F—Chlor-Alkali-Mercury Cells Pollutant or pollutant... pounds per 1,000 lb) of product TSS 0.64 0.32 Mercury (T) 0.00023 0.00010 Total Residual Chlorine 0.0032...

40 CFR 415.65 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Any new source subject to this subpart and using the mercury cell process must achieve the following new source performance standards (NSPS): Subpart F—Chlor-Alkali-Mercury Cells Pollutant or pollutant... pounds per 1,000 lb) of product TSS 0.64 0.32 Mercury (T) 0.00023 0.00010 Total Residual Chlorine 0.0032...

40 CFR 415.65 - New source performance standards (NSPS).

Code of Federal Regulations, 2014 CFR

2014-07-01

...) Any new source subject to this subpart and using the mercury cell process must achieve the following new source performance standards (NSPS): Subpart F—Chlor-Alkali-Mercury Cells Pollutant or pollutant... pounds per 1,000 lb) of product TSS 0.64 0.32 Mercury (T) 0.00023 0.00010 Total Residual Chlorine 0.0032...

40 CFR 415.65 - New source performance standards (NSPS).

Code of Federal Regulations, 2013 CFR

2013-07-01

...) Any new source subject to this subpart and using the mercury cell process must achieve the following new source performance standards (NSPS): Subpart F—Chlor-Alkali-Mercury Cells Pollutant or pollutant... pounds per 1,000 lb) of product TSS 0.64 0.32 Mercury (T) 0.00023 0.00010 Total Residual Chlorine 0.0032...

40 CFR 415.65 - New source performance standards (NSPS).

Code of Federal Regulations, 2012 CFR

2012-07-01

...) Any new source subject to this subpart and using the mercury cell process must achieve the following new source performance standards (NSPS): Subpart F—Chlor-Alkali-Mercury Cells Pollutant or pollutant... pounds per 1,000 lb) of product TSS 0.64 0.32 Mercury (T) 0.00023 0.00010 Total Residual Chlorine 0.0032...

40 CFR 415.675 - New source performance standards (NSPS):

Code of Federal Regulations, 2010 CFR

2010-07-01

... GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Zinc Chloride Production... achieve the following new source performance standards (NSPS): Subpart BO—Zinc Chloride Pollutant or... consecutive days Milligrams per liter (mg/l) TSS 28 17 Arsenic (T) 3.0 1.0. Zinc (T) 2.3 0.76 Lead (T) 0.18 0...

40 CFR 415.675 - New source performance standards (NSPS):

Code of Federal Regulations, 2011 CFR

2011-07-01

... GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Zinc Chloride Production... achieve the following new source performance standards (NSPS): Subpart BO—Zinc Chloride Pollutant or... consecutive days Milligrams per liter (mg/l) TSS 28 17 Arsenic (T) 3.0 1.0. Zinc (T) 2.3 0.76 Lead (T) 0.18 0...

40 CFR 466.13 - New source performance standards.

Code of Federal Regulations, 2011 CFR

2011-07-01

... and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units—pounds... Nickel 2.46 0.31 1.29 0.16 Zinc 2.09 0.27 0.86 0.11 Aluminum 6.21 0.78 2.54 0.32 Iron 5.74 0.72 2.87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within the range 7...

40 CFR 466.13 - New source performance standards.

Code of Federal Regulations, 2010 CFR

2010-07-01

... and grease 100.0 12.60 100.0 12.60 TSS 150.0 18.91 120.0 15.12 pH (1) (1) (1) (1) English units—pounds... Nickel 2.46 0.31 1.29 0.16 Zinc 2.09 0.27 0.86 0.11 Aluminum 6.21 0.78 2.54 0.32 Iron 5.74 0.72 2.87 0.36 Oil and grease 20.48 2.58 20.48 2.58 TSS 30.72 3.87 24.58 3.10 pH (1) (1) (1) (1) 1 Within the range 7...

High-throughput detection of RNA processing in bacteria.

PubMed

Gill, Erin E; Chan, Luisa S; Winsor, Geoffrey L; Dobson, Neil; Lo, Raymond; Ho Sui, Shannan J; Dhillon, Bhavjinder K; Taylor, Patrick K; Shrestha, Raunak; Spencer, Cory; Hancock, Robert E W; Unrau, Peter J; Brinkman, Fiona S L

2018-03-27

Understanding the RNA processing of an organism's transcriptome is an essential but challenging step in understanding its biology. Here we investigate with unprecedented detail the transcriptome of Pseudomonas aeruginosa PAO1, a medically important and innately multi-drug resistant bacterium. We systematically mapped RNA cleavage and dephosphorylation sites that result in 5'-monophosphate terminated RNA (pRNA) using monophosphate RNA-Seq (pRNA-Seq). Transcriptional start sites (TSS) were also mapped using differential RNA-Seq (dRNA-Seq) and both datasets were compared to conventional RNA-Seq performed in a variety of growth conditions. The pRNA-Seq library revealed known tRNA, rRNA and transfer-messenger RNA (tmRNA) processing sites, together with previously uncharacterized RNA cleavage events that were found disproportionately near the 5' ends of transcripts associated with basic bacterial functions such as oxidative phosphorylation and purine metabolism. The majority (97%) of the processed mRNAs were cleaved at precise codon positions within defined sequence motifs indicative of distinct endonucleolytic activities. The most abundant of these motifs corresponded closely to an E. coli RNase E site previously established in vitro. Using the dRNA-Seq library, we performed an operon analysis and predicted 3159 potential TSS. A correlation analysis uncovered 105 antiparallel pairs of TSS that were separated by 18 bp from each other and were centered on single palindromic TAT(A/T)ATA motifs (likely - 10 promoter elements), suggesting that, consistent with previous in vitro experimentation, these sites can initiate transcription bi-directionally and may thus provide a novel form of transcriptional regulation. TSS and RNA-Seq analysis allowed us to confirm expression of small non-coding RNAs (ncRNAs), many of which are differentially expressed in swarming and biofilm formation conditions. This study uses pRNA-Seq, a method that provides a genome-wide survey of RNA processing, to study the bacterium Pseudomonas aeruginosa and discover extensive transcript processing not previously appreciated. We have also gained novel insight into RNA maturation and turnover as well as a potential novel form of transcription regulation. NOTE: All sequence data has been submitted to the NCBI sequence read archive. Accession numbers are as follows: [NCBI sequence read archive: SRX156386, SRX157659, SRX157660, SRX157661, SRX157683 and SRX158075]. The sequence data is viewable using Jbrowse on www.pseudomonas.com .

Emerging investigator series: the effect of wildfire on streamwater mercury and organic carbon in a forested watershed in the southeastern United States.

PubMed

Jensen, Allison M; Scanlon, Todd M; Riscassi, Ami L

2017-12-13

Wildfires alter forested ecosystems, which include large stores of mercury (Hg) and organic carbon, two compounds that are closely linked in vegetation, soils, and streamwater. Studies have shown that wildfires release elevated levels of mercury to the atmosphere which can be locally redeposited and leave charred organic material (vegetation and litter) on the soil surface. Both can contribute to the elevated mobilization of Hg into lakes and streams. However, no studies have conducted a detailed examination of hydrological transport of Hg following a wildfire. This study investigates the coupled transport of mercury and carbon at Twomile Run, a headwater stream located in the forested mountains of Shenandoah National Park, in the year following a low-severity wildfire. Weekly baseflow samples and bi-hourly high-flow storm samples were analyzed for dissolved and particulate mercury (Hg D and Hg P , respectively), dissolved organic carbon (DOC), UV absorbance at 254 nm (UV 254 , surrogate for DOC quantity and character), and total suspended solids (TSS), and were compared with identical measurements taken from a nearby unburned watershed. For all flow conditions sampled at the burned site (which did not include the 2 months following the fire), streamwater Hg D and DOC concentrations, and corresponding UV 254 , were similar to the unburned system. TSS concentrations varied between sites but overall differences were relatively small in magnitude and likely attributable to site differences rather than fire effects. Notably, the Hg P per unit of TSS at the burned site was an order of magnitude higher than the unburned site (2.66 and 0.13 ng Hg P per mg TSS, respectively) for 8 months following the fire, resulting in elevated Hg P concentrations for the range of flow conditions, after which there was a rapid return to non-disturbed conditions. Streamwater total Hg fluxes roughly doubled (0.55 to 1.04 μg m -2 yr -1 ) as a consequence of the fire, indicating that in addition to changing atmospheric and terrestrial Hg cycling, fires can rapidly and significantly alter the streamwater Hg which has implication for downstream ecosystems. These findings are particularly relevant as the occurrence and severity of wildfires are expected to increase in the mid-latitudes in response to climate change.

Transcription-associated processes cause DNA double-strand breaks and translocations in neural stem/progenitor cells.

PubMed

Schwer, Bjoern; Wei, Pei-Chi; Chang, Amelia N; Kao, Jennifer; Du, Zhou; Meyers, Robin M; Alt, Frederick W

2016-02-23

High-throughput, genome-wide translocation sequencing (HTGTS) studies of activated B cells have revealed that DNA double-strand breaks (DSBs) capable of translocating to defined bait DSBs are enriched around the transcription start sites (TSSs) of active genes. We used the HTGTS approach to investigate whether a similar phenomenon occurs in primary neural stem/progenitor cells (NSPCs). We report that breakpoint junctions indeed are enriched around TSSs that were determined to be active by global run-on sequencing analyses of NSPCs. Comparative analyses of transcription profiles in NSPCs and B cells revealed that the great majority of TSS-proximal junctions occurred in genes commonly expressed in both cell types, possibly because this common set has higher transcription levels on average than genes transcribed in only one or the other cell type. In the latter context, among all actively transcribed genes containing translocation junctions in NSPCs, those with junctions located within 2 kb of the TSS show a significantly higher transcription rate on average than genes with junctions in the gene body located at distances greater than 2 kb from the TSS. Finally, analysis of repair junction signatures of TSS-associated translocations in wild-type versus classical nonhomologous end-joining (C-NHEJ)-deficient NSPCs reveals that both C-NHEJ and alternative end-joining pathways can generate translocations by joining TSS-proximal DSBs to DSBs on other chromosomes. Our studies show that the generation of transcription-associated DSBs is conserved across divergent cell types.

The Results of Surgical Treatment of Cushing Tumors in the Republic of Uzbekistan: Establishing Transsphenoidal Surgery in A Developing Nation.

PubMed

Powell, Michael P; Narimova, Gulshekra J; Halimova, Zamira J

2017-01-01

Transsphenoidal surgery (TSS) was introduced into the Republic of Uzbekistan in 2005 and has been developing since then. The principal center for the management of all pituitary disease is a single site for a nation with a population of approximately 30 million. Results in surgery for Cushing disease are a marker of surgical technical skill in TSS. All previously published series come from the developed world, where sophisticated investigations and management are available. Many of these investigations are not available in Uzbekistan. This mixed series of 154 patients from 2000 to 2013 presents the data from Uzbekistan. The management of Cushing disease before the introduction of TSS was with unilateral adrenalectomy, a procedure virtually unknown in the West. It reduces cortisol burden but is, in the long-term, only a temporary measure because it allows the tumor to continue to grow. The results of this procedure are presented and show that in the short-term, surprisingly reasonable remission is obtained. These results are contrasted with the experience with TSS, for which the impact of the input from an experienced team including an endocrinologist and neurosurgeon from the United Kingdom allowed a transformation in management and enabled outcomes to match what can be expected in the West. TSS for Cushing's Disease achieved an early remission of 68.7% (early morning cortisol of <50 nmol/L), although 4 patients relapsed. Copyright © 2016 Elsevier Inc. All rights reserved.

Contributions of wastewater, runoff and sewer deposit erosion to wet weather pollutant loads in combined sewer systems.

PubMed

Gasperi, J; Gromaire, M C; Kafi, M; Moilleron, R; Chebbo, G

2010-12-01

An observatory of urban pollutants was created in Paris for the purpose of assessing the dynamics of wastewater and wet weather flow (WW and WWF) pollutant loads within combined sewers. This observatory is composed of six urban catchments, covering land areas ranging in size from 42 ha to 2581 ha. For a wide array of parameters including total suspended solids (TSS), chemical and biochemical oxygen demand (COD and BOD(5)), total organic carbon (TOC), total Kjeldahl nitrogen (TKN), heavy metals (Cu and Zn) and polycyclic aromatic hydrocarbons (PAHs), this article is intended to evaluate the contributions of wastewater, runoff and in-sewer processes to WWF pollutant loads through the use of an entry-exit mass balance approach. To achieve this objective, a total of 16 rain events were sampled on these sites between May 2003 and February 2006. This study has confirmed that at the considered catchment scale (i.e. from 42 ha to 2581 ha) the production and transfer processes associated with WWF pollutant loads do not vary with basin scale. Entry-exit chemical mass balances over all catchments and for a large number of rain events indicate that wastewater constitutes the main source of organic and nitrogenous pollution, while runoff is the predominant source of Zn. For Cu, PAHs and TSS, the calculation underscores the major role played by in-sewer processes, specifically by sediment erosion, as a source of WWF pollution. A significant loss of dissolved metals was also observed during their transfer within the sewer network, likely as a consequence of the adsorption of dissolved metals on TSS and/or on sewer deposits. Moreover, the nature of eroded particles was examined and compared to the various sewer deposits. This comparison has highlighted that such particles exhibit similar organic and PAH contents to those measured in the organic layer, thus suggesting that the deposit eroded during a wet weather period is organic and of a nature comparable to the organic layer. Despite the extent of initial field investigations, no organic deposit was observed to be present on sewer lines within the catchments, which implies that this organic deposit is probably present in another form or to be found elsewhere in the main trunks. Copyright © 2010 Elsevier Ltd. All rights reserved.

Untreated runoff quality from roof and road surfaces in a low intensity rainfall climate.

PubMed

Charters, Frances J; Cochrane, Thomas A; O'Sullivan, Aisling D

2016-04-15

Sediment and heavy metals in stormwater runoff are key pollutants of urban waterways, and their presence in stormwater is driven by climatic factors such as rainfall intensity. This study describes the total suspended solids (TSS) and heavy metal concentrations found in runoff from four different urban surfaces within a residential/institutional catchment, in a climate where rainfall is typically of low intensity (<5.1mm·h(-1)). The results were compared to untreated runoff quality from a compilation of international studies. The road runoff had the highest TSS concentrations, while copper and galvanized roof runoff had the highest copper and zinc concentrations, respectively. Pollutant concentrations were found to be significantly different between surfaces; quantification and prediction of pollutant contributions from urban surfaces should thus take account of the different surface materials, instead of being aggregated into more generalized categories such as land use. The TSS and heavy metal concentrations were found to be at the low to medium end of ranges observed internationally, except for total copper and zinc concentrations generated by dissolution of copper and galvanized roofing material respectively; these concentrations were at least as high as those reported internationally. TSS wash-off from the roofs was seen to be a source-limited process, where all available TSS is washed off during the rain event despite the low intensity rainfall, whereas both road TSS and heavy metals wash-off from roof and road surfaces appeared to all be transport-limited and therefore some carryover of pollutants occurs between rain events. A first flush effect was seen from most surfaces for TSS, but not for heavy metals. This study demonstrates that in low intensity rainfall climates, quantification of untreated runoff quality from key individual surface types in a catchment are needed to enable development of targeted and appropriately sized stormwater treatment systems. Copyright © 2016 Elsevier B.V. All rights reserved.

Human-Specific Histone Methylation Signatures at Transcription Start Sites in Prefrontal Neurons

PubMed Central

Cheung, Iris; Bharadwaj, Rahul; Chou, Hsin-Jung; Houston, Isaac B.; Peter, Cyril J.; Mitchell, Amanda C.; Yao, Wei-Dong; Myers, Richard H.; Chen, Jiang-fan; Preuss, Todd M.; Rogaev, Evgeny I.; Jensen, Jeffrey D.; Weng, Zhiping; Akbarian, Schahram

2012-01-01

Cognitive abilities and disorders unique to humans are thought to result from adaptively driven changes in brain transcriptomes, but little is known about the role of cis-regulatory changes affecting transcription start sites (TSS). Here, we mapped in human, chimpanzee, and macaque prefrontal cortex the genome-wide distribution of histone H3 trimethylated at lysine 4 (H3K4me3), an epigenetic mark sharply regulated at TSS, and identified 471 sequences with human-specific enrichment or depletion. Among these were 33 loci selectively methylated in neuronal but not non-neuronal chromatin from children and adults, including TSS at DPP10 (2q14.1), CNTN4 and CHL1 (3p26.3), and other neuropsychiatric susceptibility genes. Regulatory sequences at DPP10 and additional loci carried a strong footprint of hominid adaptation, including elevated nucleotide substitution rates and regulatory motifs absent in other primates (including archaic hominins), with evidence for selective pressures during more recent evolution and adaptive fixations in modern populations. Chromosome conformation capture at two neurodevelopmental disease loci, 2q14.1 and 16p11.2, revealed higher order chromatin structures resulting in physical contact of multiple human-specific H3K4me3 peaks spaced 0.5–1 Mb apart, in conjunction with a novel cis-bound antisense RNA linked to Polycomb repressor proteins and downregulated DPP10 expression. Therefore, coordinated epigenetic regulation via newly derived TSS chromatin could play an important role in the emergence of human-specific gene expression networks in brain that contribute to cognitive functions and neurological disease susceptibility in modern day humans. PMID:23185133

The effect of in-stream activities on the Njoro River, Kenya. Part II: Microbial water quality

NASA Astrophysics Data System (ADS)

Yillia, Paul T.; Kreuzinger, Norbert; Mathooko, Jude M.

The influence of periodic in-stream activities of people and livestock on the microbial water quality of the Njoro River in Kenya was monitored at two disturbed pools (Turkana Flats and Njoro Bridge) at the middle reaches. A total of 96 sets of samples were obtained from the two pools in six weeks during dry weather (January-April) in 2006. On each sampling day, two trips were made before and during in-stream activities and on each trip, two sets of samples were collected upstream and downstream of activities. This schedule was repeated four times each for Wednesday, Saturday and Sunday. Samples were processed for heterotrophic plate count bacteria (HPC), total coliform (TC), presumptive Escherichia coli and presumptive Enterococci. Additional samples were analysed for total suspended solids (TSS), turbidity, BOD 5 and ammonium-N. The microbial water quality deteriorated significant ( p < 0.05) downstream during activities at both pools. A similar trend was observed with the chemical indicators (TSS, turbidity, BOD 5 and ammonium-N). The two groups of indicators demonstrated high capacity for site segregation based on pollution levels. Pollution levels for specific days were not significantly different ( p > 0.05). This was incompatible with the variability of in-stream activities with specific days. The pooled data was explained largely by three significant principal components - recent pollution (PC1), metabolic activity (PC2) and residual pollution (PC3). It was concluded that the empirical site parity/disparity in the levels of microbial and non-microbial indicators reflected the diurnal periodicity of in-stream activities and the concomitant pollution they caused. However, microbial source tracking studies are required to distinguish faecal sources. In the meantime, measures should be undertaken to regulate in-stream activities along the stream and minimize the movement of livestock in the catchment.

40 CFR 464.44 - New source performance standards.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 29 2010-07-01 2010-07-01 false New source performance standards. 464...

40 CFR 464.14 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 31 2013-07-01 2013-07-01 false New source performance standards. 464...

40 CFR 464.14 - New source performance standards.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 29 2010-07-01 2010-07-01 false New source performance standards. 464...

40 CFR 464.44 - New source performance standards.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 30 2014-07-01 2014-07-01 false New source performance standards. 464...

40 CFR 464.24 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 31 2013-07-01 2013-07-01 false New source performance standards. 464...

40 CFR 464.24 - New source performance standards.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 30 2011-07-01 2011-07-01 false New source performance standards. 464...

40 CFR 464.24 - New source performance standards.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 29 2010-07-01 2010-07-01 false New source performance standards. 464...

40 CFR 464.44 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 31 2013-07-01 2013-07-01 false New source performance standards. 464...

40 CFR 464.44 - New source performance standards.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 31 2012-07-01 2012-07-01 false New source performance standards. 464...

40 CFR 464.24 - New source performance standards.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 30 2014-07-01 2014-07-01 false New source performance standards. 464...

40 CFR 464.44 - New source performance standards.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 30 2011-07-01 2011-07-01 false New source performance standards. 464...

40 CFR 464.24 - New source performance standards.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 31 2012-07-01 2012-07-01 false New source performance standards. 464...

40 CFR 464.14 - New source performance standards.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 30 2014-07-01 2014-07-01 false New source performance standards. 464...

40 CFR 464.14 - New source performance standards.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 30 2011-07-01 2011-07-01 false New source performance standards. 464...

40 CFR 464.14 - New source performance standards.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., total phenols, oil and grease, and TSS. For non-continuous dischargers, annual average mass standards.... Concentration standards and annual average mass standards shall only apply to non-continuous dischargers. (a... 40 Protection of Environment 31 2012-07-01 2012-07-01 false New source performance standards. 464...

40 CFR 406.16 - Pretreatment standards for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Wet Milling Subcategory § 406.16 Pretreatment... new corn wet milling source to be discharged to the POTW (gallons per one hour for flow and pounds per day for BOD5 and TSS). Q = average existing waste load to POTW. R = average waste load for the new...

40 CFR 406.16 - Pretreatment standards for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Wet Milling Subcategory § 406.16 Pretreatment... new corn wet milling source to be discharged to the POTW (gallons per one hour for flow and pounds per day for BOD5 and TSS). Q = average existing waste load to POTW. R = average waste load for the new...

40 CFR 406.16 - Pretreatment standards for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Wet Milling Subcategory § 406.16 Pretreatment... new corn wet milling source to be discharged to the POTW (gallons per one hour for flow and pounds per day for BOD5 and TSS). Q = average existing waste load to POTW. R = average waste load for the new...

40 CFR 418.76 - Pretreatment standard for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... GUIDELINES AND STANDARDS FERTILIZER MANUFACTURING POINT SOURCE CATEGORY Mixed and Blend Fertilizer Production... the Act for a new source within the mixed and blend fertilizer subcategory which is a user of a... BOD5 No limitations. TSS Do. pH Do. Ammonia (as N) 30 mg/l. Nitrate (as N) Do. Total phosphorus (as P...

40 CFR 418.76 - Pretreatment standard for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... GUIDELINES AND STANDARDS FERTILIZER MANUFACTURING POINT SOURCE CATEGORY Mixed and Blend Fertilizer Production... the Act for a new source within the mixed and blend fertilizer subcategory which is a user of a... BOD5 No limitations. TSS Do. pH Do. Ammonia (as N) 30 mg/l. Nitrate (as N) Do. Total phosphorus (as P...

40 CFR 418.76 - Pretreatment standard for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... GUIDELINES AND STANDARDS FERTILIZER MANUFACTURING POINT SOURCE CATEGORY Mixed and Blend Fertilizer Production... the Act for a new source within the mixed and blend fertilizer subcategory which is a user of a... BOD5 No limitations. TSS Do. pH Do. Ammonia (as N) 30 mg/l. Nitrate (as N) Do. Total phosphorus (as P...

40 CFR 418.76 - Pretreatment standard for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... GUIDELINES AND STANDARDS FERTILIZER MANUFACTURING POINT SOURCE CATEGORY Mixed and Blend Fertilizer Production... the Act for a new source within the mixed and blend fertilizer subcategory which is a user of a... BOD5 No limitations. TSS Do. pH Do. Ammonia (as N) 30 mg/l. Nitrate (as N) Do. Total phosphorus (as P...

40 CFR 418.76 - Pretreatment standard for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... GUIDELINES AND STANDARDS FERTILIZER MANUFACTURING POINT SOURCE CATEGORY Mixed and Blend Fertilizer Production... the Act for a new source within the mixed and blend fertilizer subcategory which is a user of a... BOD5 No limitations. TSS Do. pH Do. Ammonia (as N) 30 mg/l. Nitrate (as N) Do. Total phosphorus (as P...

Epidemiology and Clinical Relevance of Toxic Shock Syndrome in US Children.

PubMed

Gaensbauer, James T; Birkholz, Meghan; Smit, Michael A; Garcia, Roger; Todd, James K

2018-03-27

It is important for clinicians to recognize the contribution of toxic shock syndrome (TSS) to the overall burden of pediatric septic shock because the clinical features, optimal therapy and prognosis differ from non-TSS septic shock. We analyzed cases of pediatric septic shock reported to the Pediatric Health Information Systems (PHIS) database between 2009 and 2013 to define the clinical and demographic characteristics of pediatric TSS in the US. Using a validated ICD-9 coding strategy we identified patients with infectious shock among inpatients age 1 to 18 years, and classified cases of staphylococcal and streptococcal TSS for comparison with non-TSS cases. Of 8,226 cases of pediatric septic shock, 909 (11.1%) were classified as TSS and 562 (6.8%) were possible TSS cases. Staphylococcal TSS represented the majority (83%) of TSS cases, and occurred more commonly in females and at an older age. Compared with non-TSS septic shock, TSS had significantly lower fatality rates, disease severity, and length of hospital stay, and was present more often at the time of admission (p<0.001 for each). Streptococcal TSS was associated with poorer outcomes than staphylococcal TSS. Treatment for TSS differed from non-TSS septic shock in use of more clindamycin, vancomycin and IVIG, and less need for vasopressors. Results demonstrate a significant contribution of TSS to the burden of pediatric septic shock in the US. The findings emphasize the importance of inclusion of TSS diagnostic and therapeutic considerations in sepsis treatment protocols for children.

40 CFR 437.24 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

..., TSS, arsenic, cadmium, chromium, cobalt, copper, lead, mercury, tin, zinc, butylbenzyl phthalate, carbazole, n-decane, bis(2-ethylhexyl) phthalate, fluoranthene, and n-octadecane are the same as the...

40 CFR 437.24 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

..., TSS, arsenic, cadmium, chromium, cobalt, copper, lead, mercury, tin, zinc, butylbenzyl phthalate, carbazole, n-decane, bis(2-ethylhexyl) phthalate, fluoranthene, and n-octadecane are the same as the...

40 CFR 410.35 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Low Water Use Processing Subcategory § 410.35... product BOD5 1.4 0.7 COD 2.8 1.4 TSS 1.4 0.7 pH (1) (1) 1 Within the range 6.0 to 9.0 at all times. Water...

40 CFR 410.35 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Low Water Use Processing Subcategory § 410.35... product BOD5 1.4 0.7 COD 2.8 1.4 TSS 1.4 0.7 pH (1) (1) 1 Within the range 6.0 to 9.0 at all times. Water...

North Carolina DOT traffic separation studies. Volume I, Assessment.

DOT National Transportation Integrated Search

2004-09-01

The Federal Railroad Administration requested the Volpe National Transportation Systems Center to assess ten sites in depth that used the Traffic Separation Study (TSS) process. The assessment involved a quantitative and qualitative analysis of the i...

40 CFR 466.33 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 6.13 0.79 Oil and grease 97.24 12.60 97.24 12.60 TSS 145.86 18.91 116.69 15.12 pH (1) (1) (1) (1... 0.03 0.18 0.20 Nickel 1.10 0.14 0.74 0.10 Zinc 2.03 0.27 0.84 0.11 Aluminum 6.03 0.78 2.47 0.32 Iron 2.45 0.32 1.26 0.16 Oil and grease 19.92 2.58 19.92 2.58 TSS 29.88 3.87 23.90 3.10 pH (1) (1) (1) (1...

40 CFR 466.33 - New source performance standards.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 6.13 0.79 Oil and grease 97.24 12.60 97.24 12.60 TSS 145.86 18.91 116.69 15.12 pH (1) (1) (1) (1... 0.03 0.18 0.20 Nickel 1.10 0.14 0.74 0.10 Zinc 2.03 0.27 0.84 0.11 Aluminum 6.03 0.78 2.47 0.32 Iron 2.45 0.32 1.26 0.16 Oil and grease 19.92 2.58 19.92 2.58 TSS 29.88 3.87 23.90 3.10 pH (1) (1) (1) (1...

40 CFR 466.33 - New source performance standards.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 6.13 0.79 Oil and grease 97.24 12.60 97.24 12.60 TSS 145.86 18.91 116.69 15.12 pH (1) (1) (1) (1... 0.03 0.18 0.20 Nickel 1.10 0.14 0.74 0.10 Zinc 2.03 0.27 0.84 0.11 Aluminum 6.03 0.78 2.47 0.32 Iron 2.45 0.32 1.26 0.16 Oil and grease 19.92 2.58 19.92 2.58 TSS 29.88 3.87 23.90 3.10 pH (1) (1) (1) (1...

Turbidity - a Semi-Continuous Monitoring Option for Suspended Solids

NASA Astrophysics Data System (ADS)

Lendvay, J. M.; Rosasco, M. V.; David, K. E.

2012-12-01

Redwood Creek, a third order coastal stream flowing through Muir Woods National Monument and Golden Gate National Recreation Area in Marin County, California, was once the spawning grounds for a relatively large population of Coho Salmon (Oncorhynchus kisutch). In recent years less than 1% of historic populations have been returning to the stream. Redwood creek is currently undergoing extensive ecological restoration in an attempt to improve the spawning habitat for the salmon. The original stream path has been altered in the past to make way for development and the National Park Service has been working towards restoring much of the stream's natural functionality with the hope that the salmon population will increase. The restoration process has altered the surrounding riparian landscape in the Redwood Creek watershed. Riparian disturbance caused by vegetation and levee removal as a part of the restoration process followed by installation of seedlings raises concern about the concentration of sediments in the water. Throughout 2011-2012 three parameters for water quality were monitored at Redwood Creek. Suspended sediment concentration (SSC) and total suspended solids (TSS) measurements to determine the concentration of suspended particles in the water column at a given point in time. Turbidity, measured in Nephelometric Turbidity Units (NTU) is a measure of the water's cloudiness caused by suspended particles. Turbidity measurements are favored as they provide a semi-automated monitoring option. Therefore, development of a relationship between turbidity and SSC and TSS is desired. Water samples were analyzed for TSS and SSC using the EPA standard methods, and Turbidity was measured using a Hach 2100Q portable turbidimeter. Additional semi-continuous monitoring of turbidity was completed in situ using Hydrolab DS5X datasondes (with self-cleaning turbidity sensor). The relationship between TSS, SSC and turbidity was determined using a linear regression model for two separate sites within Redwood creek. The linear correlation coefficient values for the two sites were high for both TSS vs. Turbidity (R2=0.929) and SSC vs. Turbidity (R2=0.929). Measures of suspended particle concentrations in the water column are important indicators of water quality, as sediments can be transporters of both nutrients and contaminants alike, particularly those with high binding affinities for soils. Moreover, the relationships between TSS, SSC and turbidity may serve as an important predictor when developing a model for nutrient and contaminant dynamics in coastal California streams. Determining the best way to measure suspended solids in the water column will help elucidate the most practicable in-stream monitoring methods.

Photolytic Release of Dissolved Vanadium and Copper from Resuspended Coastal Marine Sediments

NASA Astrophysics Data System (ADS)

Skrabal, S. A.; Hammaker, S. N.; McBurney, A. W.; Avery, G. B., Jr.; Kieber, R. J.; Mead, R. N.

2016-02-01

Sunlight photolysis engenders release of dissolved vanadium (V), copper (Cu), and dissolved organic carbon (DOC) from a wide variety of resuspendable coastal marine sediments. Net photoreleases after 6 h of simulated sunlight were as high as 12 nM for Cu and as high as 15 nM for V. Release of Cu significantly correlated with sediment Cu. Photoreleased Cu (but not V) correlated with sediment Fe content, suggesting that photoreduction of Fe oxide carrier phases may be an important photoproduction mechanism for Cu. Longer term experiments showed continued release of metals that were not immediately readsorbed back onto sediments after 24 h of irradiation suggesting that photoproduced metals persist in the dissolved phase and are not immediately scavenged onto particles. Experiments utilizing differing total suspended sediment (TSS) levels show that, although higher TSS causes more photoproduction of Cu and V, the amount produced per mass of sediment is greatest at the lowest TSS. Vanadium photoproduction increased in Macondo oil-amended sediments compared to controls after a one-month incubation period suggesting that the oil may be a source of this metal to the water column. These results imply that photoproduction is an unrecognized source of the micronutrient metals Cu and V to coastal waters.

Clinical and Molecular Epidemiology of Staphylococcal Toxic Shock Syndrome in the United Kingdom.

PubMed

Sharma, Hema; Smith, Debra; Turner, Claire E; Game, Laurence; Pichon, Bruno; Hope, Russell; Hill, Robert; Kearns, Angela; Sriskandan, Shiranee

2018-02-01

Staphylococcal toxic shock syndrome (TSS) was originally described in menstruating women and linked to TSS toxin 1 (TSST-1)-producing Staphylococcus aureus. Using UK national surveillance data, we ascertained clinical, molecular and superantigenic characteristics of TSS cases. Average annual TSS incidence was 0.07/100,000 population. Patients with nonmenstrual TSS were younger than those with menstrual TSS but had the same mortality rate. Children <16 years of age accounted for 39% of TSS cases, most caused by burns and skin and soft tissue infections. Nonmenstrual TSS is now more common than menstrual TSS in the UK, although both types are strongly associated with the tst+ clonal complex (CC) 30 methicillin-sensitive S. aureus lineage, which accounted for 49.4% of all TSS and produced more TSST-1 and superantigen bioactivity than did tst+ CC30 methicillin-resistant S. aureus strains. Better understanding of this MSSA lineage and infections in children could focus interventions to prevent TSS in the future.

Clinical and Molecular Epidemiology of Staphylococcal Toxic Shock Syndrome in the United Kingdom

PubMed Central

Sharma, Hema; Smith, Debra; Turner, Claire E.; Game, Laurence; Pichon, Bruno; Hope, Russell; Hill, Robert; Kearns, Angela

2018-01-01

Staphylococcal toxic shock syndrome (TSS) was originally described in menstruating women and linked to TSS toxin 1 (TSST-1)–producing Staphylococcus aureus. Using UK national surveillance data, we ascertained clinical, molecular and superantigenic characteristics of TSS cases. Average annual TSS incidence was 0.07/100,000 population. Patients with nonmenstrual TSS were younger than those with menstrual TSS but had the same mortality rate. Children <16 years of age accounted for 39% of TSS cases, most caused by burns and skin and soft tissue infections. Nonmenstrual TSS is now more common than menstrual TSS in the UK, although both types are strongly associated with the tst+ clonal complex (CC) 30 methicillin-sensitive S. aureus lineage, which accounted for 49.4% of all TSS and produced more TSST-1 and superantigen bioactivity than did tst+ CC30 methicillin-resistant S. aureus strains. Better understanding of this MSSA lineage and infections in children could focus interventions to prevent TSS in the future. PMID:29350159

Watershed-scale Hydrology and Water Quality Impact of Switchgrass Intercropping in Southern Managed Pine Forests

NASA Astrophysics Data System (ADS)

Chescheir, G. M.; Birgand, F.; Allen, E.; Bennett, E.; Carter, T.; Dobbs, N.; Muwamba, A.; Amatya, D. M.; Youssef, M.; Nettles, J. E.

2016-12-01

The use of marginal land for cellulosic biofuel crop production is an attractive solution to preserve agricultural land for food production. The space available between rows of young loblolly pine (Pinus taeda) trees offers enough light to support growth of biofuel crops for several years. A five year field study was conducted to assess the hydrology and water quality impacts of switchgrass (Panicum virgatum) intercropping with pine trees in watersheds of the southeastern US. Paired-watershed studies were replicated in Mississippi and Alabama on upland sites, and in North Carolina on a flat lowland site. In each state, the impact of switchgrass intercropping was assessed from differences in water and nutrient yields from contiguous 20-40 ha watersheds established as: conventional young pine plantation, switchgrass intercropped in young pine plantation, switchgrass only, and mid-rotation mature pine plantation. A total of 14 watersheds were equipped with continuous flow monitoring stations, flow proportional water samplers, groundwater wells, soil moisture sensors and weather stations. Data collection continued through a two year pre-treatment period, a one year treatment period when field operations were conducted to establish switchgrass, and a two year post-treatment period when the established switchgrass was fertilized and harvested annually. Our results showed that significant increases in total suspended solids (TSS) and nitrogen (N) loading occurred during the treatment periods at the upland sites in MS and AL. During the post treatment periods, TSS and N loading decreased to levels near those observed in pretreatment. At the lowland site, only nitrogen loading was increased during the treatment period. Concentrations of TSS at the lowland site were two orders of magnitude lower than those observed at the upland sites and were not significantly affected by the treatment. Inherent flow variability between watersheds within sites made detection of subtle differences in hydrology and water quality difficult to detect. Increases in N loading were not significant in response to annual fertilization of switchgrass. Our results suggest that intercropping switchgrass in managed pine forests does not significantly change the typically benign hydrology and water quality of the managed forests.

Source-Based Modeling Of Urban Stormwater Quality Response to the Selected Scenarios Combining Future Changes in Climate and Socio-Economic Factors

NASA Astrophysics Data System (ADS)

Borris, Matthias; Leonhardt, Günther; Marsalek, Jiri; Österlund, Heléne; Viklander, Maria

2016-08-01

The assessment of future trends in urban stormwater quality should be most helpful for ensuring the effectiveness of the existing stormwater quality infrastructure in the future and mitigating the associated impacts on receiving waters. Combined effects of expected changes in climate and socio-economic factors on stormwater quality were examined in two urban test catchments by applying a source-based computer model (WinSLAMM) for TSS and three heavy metals (copper, lead, and zinc) for various future scenarios. Generally, both catchments showed similar responses to the future scenarios and pollutant loads were generally more sensitive to changes in socio-economic factors (i.e., increasing traffic intensities, growth and intensification of the individual land-uses) than in the climate. Specifically, for the selected Intermediate socio-economic scenario and two climate change scenarios (RSP = 2.6 and 8.5), the TSS loads from both catchments increased by about 10 % on average, but when applying the Intermediate climate change scenario (RCP = 4.5) for two SSPs, the Sustainability and Security scenarios (SSP1 and SSP3), the TSS loads increased on average by 70 %. Furthermore, it was observed that well-designed and maintained stormwater treatment facilities targeting local pollution hotspots exhibited the potential to significantly improve stormwater quality, however, at potentially high costs. In fact, it was possible to reduce pollutant loads from both catchments under the future Sustainability scenario (on average, e.g., TSS were reduced by 20 %), compared to the current conditions. The methodology developed in this study was found useful for planning climate change adaptation strategies in the context of local conditions.

Source-Based Modeling Of Urban Stormwater Quality Response to the Selected Scenarios Combining Future Changes in Climate and Socio-Economic Factors.

PubMed

Borris, Matthias; Leonhardt, Günther; Marsalek, Jiri; Österlund, Heléne; Viklander, Maria

2016-08-01

The assessment of future trends in urban stormwater quality should be most helpful for ensuring the effectiveness of the existing stormwater quality infrastructure in the future and mitigating the associated impacts on receiving waters. Combined effects of expected changes in climate and socio-economic factors on stormwater quality were examined in two urban test catchments by applying a source-based computer model (WinSLAMM) for TSS and three heavy metals (copper, lead, and zinc) for various future scenarios. Generally, both catchments showed similar responses to the future scenarios and pollutant loads were generally more sensitive to changes in socio-economic factors (i.e., increasing traffic intensities, growth and intensification of the individual land-uses) than in the climate. Specifically, for the selected Intermediate socio-economic scenario and two climate change scenarios (RSP = 2.6 and 8.5), the TSS loads from both catchments increased by about 10 % on average, but when applying the Intermediate climate change scenario (RCP = 4.5) for two SSPs, the Sustainability and Security scenarios (SSP1 and SSP3), the TSS loads increased on average by 70 %. Furthermore, it was observed that well-designed and maintained stormwater treatment facilities targeting local pollution hotspots exhibited the potential to significantly improve stormwater quality, however, at potentially high costs. In fact, it was possible to reduce pollutant loads from both catchments under the future Sustainability scenario (on average, e.g., TSS were reduced by 20 %), compared to the current conditions. The methodology developed in this study was found useful for planning climate change adaptation strategies in the context of local conditions.

40 CFR 463.24 - New source performance standards.

Code of Federal Regulations, 2014 CFR

2014-07-01

... usage flow rate for cleaning processes at a new source times the following pollutant concentrations: Subpart B [Cleaning water] Concentration used to calculate NSPS Pollutant or pollutant property Maximum for any 1 day (mg/l) Maximum for monthly average (mg/l) BOD5 49 22 Oil and Grease 71 17 TSS 117 36 pH...

40 CFR 463.24 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

... usage flow rate for cleaning processes at a new source times the following pollutant concentrations: Subpart B [Cleaning water] Concentration used to calculate NSPS Pollutant or pollutant property Maximum for any 1 day (mg/l) Maximum for monthly average (mg/l) BOD5 49 22 Oil and Grease 71 17 TSS 117 36 pH...

Land Use Land Cover Changes in Detection of Water Quality: A Study Based on Remote Sensing and Multivariate Statistics.

PubMed

Hua, Ang Kean

2017-01-01

Malacca River water quality is affected due to rapid urbanization development. The present study applied LULC changes towards water quality detection in Malacca River. The method uses LULC, PCA, CCA, HCA, NHCA, and ANOVA. PCA confirmed DS, EC, salinity, turbidity, TSS, DO, BOD, COD, As, Hg, Zn, Fe, E. coli , and total coliform. CCA confirmed 14 variables into two variates; first variate involves residential and industrial activities; and second variate involves agriculture, sewage treatment plant, and animal husbandry. HCA and NHCA emphasize that cluster 1 occurs in urban area with Hg, Fe, total coliform, and DO pollution; cluster 3 occurs in suburban area with salinity, EC, and DS; and cluster 2 occurs in rural area with salinity and EC. ANOVA between LULC and water quality data indicates that built-up area significantly polluted the water quality through E. coli , total coliform, EC, BOD, COD, TSS, Hg, Zn, and Fe, while agriculture activities cause EC, TSS, salinity, E. coli , total coliform, arsenic, and iron pollution; and open space causes contamination of turbidity, salinity, EC, and TSS. Research finding provided useful information in identifying pollution sources and understanding LULC with river water quality as references to policy maker for proper management of Land Use area.

40 CFR 426.115 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (the fluoride and lead limitations are applicable to the abrasive polishing and acid polishing waste water streams while the TSS, oil, and pH limitations are applicable to the entire process waste water...

Influence of total soluble salt concentration on growth and elemental concentration of winged bean seedlings, Psophocarpus tetragonolobus (L. ) DC

DOE Office of Scientific and Technical Information (OSTI.GOV)

Csizinszky, A.A.

Winged bean (Psophocarpus tetragonolobus) (L.) DC) seedlings of the accession TPT-1, were grown in a greenhouse with graded, balanced total soluble salt (TSS) concentrations. After 45 days, plant height increased quadratically, with a maximum (149 cm) at 3000 ppm TSS. Seedlings were shortest at 1000 and 10,000 ppm TSS, 44.0 and 79.0 cm, respectively. Fresh weight of shoots increased quadratically with greatest weight, 29.03 g, at 5000 ppm TSS. Percent dry matter increased linearly with increasing TSS. Concentration of N, K and P increased quadratically with an increase in the TSS concentration in the growth medium. Concentration of Ca decreasedmore » quadratically with increasing TSS. Among the micronutrients, Fe and Mo concentration was quadratic, both elements were highest in the seedlings at 1000 and 10,000 ppm TSS rates. Concentrations of Mn and Zn increased linearly with increasing TSS. Winged bean seedlings at the 1000 to 3000 ppm TSS rates had spindly stems and a sparse, yellow foliage, typical for winged bean seedlings observed in the field during the first 4 to 5 weeks of growth. Seedlings at the 4000 and 5000 ppm TSS rates had sturdy stems and an abundant green foliage. At higher TSS concentrations, 5000 to 10,000 ppm TSS, seedlings had short intermodes and dark green foliage.« less

Toxic shock syndrome surveillance in UK children.

PubMed

Adalat, S; Dawson, T; Hackett, S J; Clark, J E

2014-12-01

Toxic shock syndrome (TSS) is an acute toxin-mediated illness caused by toxin-producing strains of Staphylococcus aureus and Streptococcus pyogenes. There is no recent data regarding incidence, management and mortality of TSS in UK children. Consultants from paediatric and burns units in the UK and Ireland, reported cases of TSS seen between November 2008 and December 2009, via the British Paediatric Surveillance Unit. Respondents were sent questionnaires requesting detailed information about TSS cases. Established criteria were used to divide cases into staphylococcal or streptococcal TSS. Forty-nine cases were identified overall; 29 cases of streptococcal TSS (18 confirmed and 11 probable) and 20 cases of staphylococcal TSS (15 confirmed and 5 probable). The incidence of TSS children in the UK & the Republic of Ireland was calculated to be 0.38 per 100 000 children. Children with staphylococcal TSS were older than those with streptococcal TSS (9.5 vs 3.8 years; p<0.003). Paediatric intensive care facilities were used for 78% of cases (invasive ventilatory support 69%; inotropic support 67%; haemofiltration 12%). Agents with antitoxin effects were underused; clindamycin 67%, intravenous immunoglobulin (IVIG) 20%, fresh frozen plasma 40%. There were eight deaths, all in the streptococcal group (28% of streptococcal cases)-none were given IVIG. Streptococcal TSS was as frequent as staphylococcal TSS, contrasting with previous literature. Children with streptococcal TSS had a higher mortality than those with staphylococcal TSS (28% vs 0%; p<0.05). Recommended immunomodulatory agents (IVIG and clindamycin) were underused. This study highlights the need for a guideline to improve management of TSS in children. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Review of Oceanographic and Geochemical Data Collected in Massachusetts Bay during a Large Discharge of Total Suspended Solids from Boston's Sewage-Treatment System and Ocean Outfall in August 2002

USGS Publications Warehouse

Bothner, Michael H.; Butman, Bradford; Casso, Michael A.

2010-01-01

During the period August 14-23, 2002, the discharge of total suspended solids (TSS) from the Massachusetts Water Resources Authority sewage-treatment plant ranged from 32 to 132 milligrams per liter, causing the monthly average discharge to exceed the limit specified in the National Pollution Discharge Elimination System permit. Time-series monitoring data collected by the U.S. Geological Survey in western Massachusetts Bay were examined to evaluate changes in environmental conditions during and after this exceedance event. The rate of sediment trapping and the concentrations of near-bottom suspended sediment measured near the outfall in western Massachusetts Bay increased during this period. Because similar increases in sediment-trapping rate were observed in the summers of 2003 and 2004, however, the increase in 2002 cannot be definitively attributed to the increased TSS discharge. Concentrations of copper and silver in trapped sediment collected 10 and 20 days following the 2002 TSS event were elevated compared to those in pre-event samples. Maximum concentrations were less than 50 percent of toxicity guidelines. Photographs of surficial bottom sediments obtained before and after the TSS event do not show sediment accumulation on the sea floor. Concentrations of silver, Clostridium perfringens, and clay in surficial bottom sediments sampled 10 weeks after the discharge event at a depositional site 3 kilometers west of the outfall were unchanged from those in samples obtained before the event. Simulation of the TSS event by using a coupled hydrodynamic-wave-sediment-transport model could enhance understanding of these observations and of the effects of the exceedance on the local marine environment.

40 CFR 463.34 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

... pollutant concentrations: Subpart C [Finishing water] Concentration used to calculate NSPS Pollutant or pollutant property Maximum for any 1 day (mg/l) Maximum for monthly average (mg/l) TSS 130 37 pH (1) (1) 1...

COMPARISON OF PREDICTED AND OBSERVED PLUME TRENDS AT CONTAMINATED SITES

EPA Science Inventory

Over 45 natural attenuation treatability studies (TSs) were performed from 1993 to 1999 by Parsons Corporation (Parsons) for the Technology Transfer Division of the Air Force Center for Environmental Excellence (AFCEE/ERT) in conjunction with researchers from the US EPA - NRMRL. ...

40 CFR 406.45 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour Milling Subcategory... limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.015 0.005 TSS 0.0099 .0033 pH (1) (1) English units...

40 CFR 406.45 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour Milling Subcategory... limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.015 0.005 TSS 0.0099 .0033 pH (1) (1) English units...

40 CFR 406.65 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice Processing Subcategory... limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of rice) BOD5 0.21 0.07 TSS 0.09 .03 pH (1) (1) English units (pounds per...

40 CFR 406.65 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice Processing Subcategory... limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of rice) BOD5 0.21 0.07 TSS 0.09 .03 pH (1) (1) English units (pounds per...

40 CFR 406.45 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour Milling Subcategory... limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.015 0.005 TSS 0.0099 .0033 pH (1) (1) English units...

40 CFR 406.65 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice Processing Subcategory... limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of rice) BOD5 0.21 0.07 TSS 0.09 .03 pH (1) (1) English units (pounds per...

40 CFR 406.45 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour Milling Subcategory... limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.015 0.005 TSS 0.0099 .0033 pH (1) (1) English units...

40 CFR 463.14 - New source performance standards.

Code of Federal Regulations, 2011 CFR

2011-07-01

... contact cooling and heating water processes at a new source times the following pollutant concentrations: Subpart A [Contact cooling and heating water] Concentration used to calculate NSPS Pollutant or pollutant property Maximum for any 1 day (mg/l) BOD5 26 Oil and grease 29 TSS 19 pH (1) 1 Within the range of 6.0 to...

Treatment and potential reuse of greywater from schools: a pilot study.

PubMed

Alsulaili, Abdalrahman D; Hamoda, Mohamed F; Al-Jarallah, Rawa; Alrukaibi, Duaij

2017-05-01

This study presented performance data on a low cost and easy maintenance pilot system for on-site treatment and reuse of water collected from wash sinks and fountains, as major sources of greywater (GW) at schools. Various treatment options were studied including screening, sand filtration, chlorination, and UV disinfection operated at different flow rates. Results showed that filtration operated at low rates is very effective in total suspended solids (TSS) removal, while UV proved to be more effective than chlorination for reduction of biochemical oxygen demand (BOD), chemical oxygen demand (COD), and total coliforms. Removal efficiencies up to 63%, 30% and 20% were obtained for TSS, COD and BOD, respectively and reductions of log TC (CFU/100 ml) from 6.5 to 2 were obtained at a filtration rate of 14 m 3 /d·m 2 . Treated effluent satisfied WHO standards for reclaimed water reuse in landscape irrigation and toilet flushing. The filtration-UV system is robust, showing the best and most reliable performance for low and high strength GW treatment even under a 10-fold increase in flow rate. A 5 m 3 /d pilot plant was developed for schools having 500 students and detailed cost-benefit analysis indicated a net saving value, a surplus of $1,600 per year, and pay back after 6 years and 11 months.

Structural Characterization and Oligomerization of the TssL Protein, a Component Shared by Bacterial Type VI and Type IVb Secretion Systems*

PubMed Central

Durand, Eric; Zoued, Abdelrahim; Spinelli, Silvia; Watson, Paul J. H.; Aschtgen, Marie-Stéphanie; Journet, Laure; Cambillau, Christian; Cascales, Eric

2012-01-01

The Type VI secretion system (T6SS) is a macromolecular system distributed in Gram-negative bacteria, responsible for the secretion of effector proteins into target cells. The T6SS has a broad versatility as it can target both eukaryotic and prokaryotic cells. It is therefore involved in host pathogenesis or killing neighboring bacterial cells to colonize a new niche. At the architecture level, the T6SS core apparatus is composed of 13 proteins, which assemble in two subcomplexes. One of these subcomplexes, composed of subunits that share structural similarities with bacteriophage tail and baseplate components, is anchored to the cell envelope by the membrane subcomplex. This latter is constituted of at least three proteins, TssL, TssM, and TssJ. The crystal structure of the TssJ outer membrane lipoprotein and its interaction with the inner membrane TssM protein have been recently reported. TssL and TssM share sequence homology and characteristics with two components of the Type IVb secretion system (T4bSS), IcmH/DotU and IcmF, respectively. In this study, we report the crystal structure of the cytoplasmic domain of the TssL inner membrane protein from the enteroaggregative Escherichia coli Sci-1 T6SS. It folds as a hook-like structure composed of two three-helix bundles. Two TssL molecules associate to form a functional complex. Although the TssL trans-membrane segment is the main determinant of self-interaction, contacts between the cytoplasmic domains are required for TssL function. Based on sequence homology and secondary structure prediction, we propose that the TssL structure is the prototype for the members of the TssL and IcmH/DotU families. PMID:22371492

Cap-proximal nucleotides via differential eIF4E binding and alternative promoter usage mediate translational response to energy stress.

PubMed

Tamarkin-Ben-Harush, Ana; Vasseur, Jean-Jacques; Debart, Françoise; Ulitsky, Igor; Dikstein, Rivka

2017-02-08

Transcription start-site (TSS) selection and alternative promoter (AP) usage contribute to gene expression complexity but little is known about their impact on translation. Here we performed TSS mapping of the translatome following energy stress. Assessing the contribution of cap-proximal TSS nucleotides, we found dramatic effect on translation only upon stress. As eIF4E levels were reduced, we determined its binding to capped-RNAs with different initiating nucleotides and found the lowest affinity to 5'cytidine in correlation with the translational stress-response. In addition, the number of differentially translated APs was elevated following stress. These include novel glucose starvation-induced downstream transcripts for the translation regulators eIF4A and Pabp, which are also translationally-induced despite general translational inhibition. The resultant eIF4A protein is N-terminally truncated and acts as eIF4A inhibitor. The induced Pabp isoform has shorter 5'UTR removing an auto-inhibitory element. Our findings uncovered several levels of coordination of transcription and translation responses to energy stress.

Resuspended contaminated sediments cause sublethal stress to oysters: A biomarker differentiates total suspended solids and contaminant effects.

PubMed

Edge, Katelyn J; Dafforn, Katherine A; Simpson, Stuart L; Ringwood, Amy H; Johnston, Emma L

2015-06-01

Resuspended contaminated sediments represent an important route of contaminant exposure for aquatic organisms. During resuspension events, filter-feeding organisms are exposed to contaminants, in both the dissolved form (at the gills) and the particulate form (in the digestive system). In addition, these organisms must manage the physical stress associated with an increase in total suspended solids (TSS). To date, few studies have experimentally compared the contributions to biological stress of contaminated and clean suspended solids. The authors mixed field-collected sediments (<63 μm) from clean and contaminated field sites to create 4 treatments of increasing metal concentrations. Sydney rock oysters were then exposed to sediment treatments at different TSS concentrations for 4 d, and cellular biomarkers (lysosomal membrane stability, lipid peroxidation, and glutathione) were measured to evaluate sublethal toxicity. Lysosomal membrane stability was the most sensitive biomarker for distinguishing effects from resuspended contaminated sediments, as increasing amounts of contaminated TSS increased lysosomal membrane destabilization. The authors' results illustrate the importance of considering contaminant exposures from resuspended sediments when assessing the toxicity of contaminants to aquatic organisms. © 2015 SETAC.

Cold climate performance analysis of on-site domestic wastewater treatment systems.

PubMed

Williamson, Eric

2010-06-01

Household on-site septic systems with secondary wastewater treatment in Anchorage, Alaska, were sampled and analyzed for performance parameters during the winter to spring months. System types included intermittent dosing sand filters (ISF), three types of recirculating trickling filters (RTF), and suspended-growth aeration tanks. Total nitrogen from the trickling filter and aeration tank effluent was fairly uniform, at approximately 30 mg/L. Total suspended solids (TSS) means were mostly less than 15 mg/L. The 5-day biochemical oxygen demand (BODs) showed considerable variability, with means ranging from 9.2 mg/ L for ISFs up to 39.5 mg/L for one type of RTF, even though this type has shown excellent results in several test programs. The data suggested that effluent temperature within the sample range had almost no effect on effluent concentrations of BOD5 or TSS and only a small effect on the removal of total nitrogen. Non-climatic factors were probably of equal importance to treatment results.

Anaerobic on-site treatment of black water and dairy parlour wastewater in UASB-septic tanks at low temperatures.

PubMed

Luostarinen, Sari A; Rintala, Jukka A

2005-01-01

Anaerobic on-site treatment of synthetic black water (BW) and dairy parlour wastewater (DPWW) was studied in two-phased upflow anaerobic sludge blanket (UASB)-septic tanks at low temperatures (10-20 degrees C). At all temperatures, total chemical oxygen demand (COD(t)) removal was above 90% with BW and above 80% with DPWW and removal of total suspended solids (TSS) above 90% with both wastewaters. Moreover, dissolved COD (COD(dis)) removal was approx. 70% with both wastewaters indicating good biological activity of the sludges. With BW, a single-phased reactor was found sufficient for good COD removals, while with DPWW, a two-phased process was required. Temperature optimum of reactor sludges was still 35 degrees C after long (398d) operation. Most of the nutrients from BW were removed with TSS, while with DPWW nutrient removal was low. In conclusion, UASB-septic tank was found feasible for (pre)treatment of BW and DPWW at low temperatures.

Highway runoff quality models for the protection of environmentally sensitive areas

NASA Astrophysics Data System (ADS)

Trenouth, William R.; Gharabaghi, Bahram

2016-11-01

This paper presents novel highway runoff quality models using artificial neural networks (ANN) which take into account site-specific highway traffic and seasonal storm event meteorological factors to predict the event mean concentration (EMC) statistics and mean daily unit area load (MDUAL) statistics of common highway pollutants for the design of roadside ditch treatment systems (RDTS) to protect sensitive receiving environs. A dataset of 940 monitored highway runoff events from fourteen sites located in five countries (Canada, USA, Australia, New Zealand, and China) was compiled and used to develop ANN models for the prediction of highway runoff suspended solids (TSS) seasonal EMC statistical distribution parameters, as well as the MDUAL statistics for four different heavy metal species (Cu, Zn, Cr and Pb). TSS EMCs are needed to estimate the minimum required removal efficiency of the RDTS needed in order to improve highway runoff quality to meet applicable standards and MDUALs are needed to calculate the minimum required capacity of the RDTS to ensure performance longevity.

Retention of duplicated ITAM-containing transmembrane signaling subunits in the tetraploid amphibian species Xenopus laevis

PubMed Central

Guselnikov, S.V.; Grayfer, L.; De Jesús Andino, F.; Rogozin, I.B.; Robert, J.; Taranin, A.V.

2015-01-01

The ITAM-bearing transmembrane signaling subunits (TSS) are indispensable components of activating leukocyte receptor complexes. The TSS-encoding genes map to paralogous chromosomal regions, which are thought to arise from ancient genome tetraploidization(s). To assess a possible role of tetraploidization in the TSS evolution, we studied TSS and other functionally linked genes in the amphibian species Xenopus laevis whose genome was duplicated about 40 MYR ago. We found that X. laevis has retained a duplicated set of sixteen TSS genes, all except one being transcribed. Furthermore, duplicated TCRα loci and genes encoding TSS-coupling protein kinases have also been retained. No clear evidence for functional divergence of the TSS paralogs was obtained from gene expression and sequence analyses. We suggest that the main factor of maintenance of duplicated TSS genes in X. laevis was a protein dosage effect and that this effect might have facilitated the TSS set expansion in early vertebrates. PMID:26170006

Mitigation and treatment of pollutants from railway and highway runoff by pocket wetland system; A case study.

PubMed

Senduran, Cem; Gunes, Kemal; Topaloglu, Duygu; Dede, Omer Hulusi; Masi, Fabio; Kucukosmanoglu, Ozen Arli

2018-08-01

This study performed in Sapanca Lake catchment area, used as a drinking water resource. Two highways located at northern and southern shores, and a railway at its south are significant sources of pollution. As a possible solution for protecting water quality a pocket wetland constructed and operated. Performances statistically interpreted by Spearman's Correlation test and univariate analysis of variance on collected data. The mean removal efficiencies obtaited were 52% (TSS), 4% (Nitrate), 26% (TN), -5% (TOC), 63% (TP), 4.5% (Chloride), 3% (Sulfate), 33% (Cr), 39% (Co), -19.5% (Ni), 7% (Cu), 55% (Zn), 36% (As), 38% (Cd) and 18% (Pb). TSS removal was in positive significant medium correlation with Co, Cu, Zn, and Pb removal respectively (p < 0.05). Other statistically significant positive high correlations calculated between removal efficiency of Nitrate-TN, Chloride-Sulfate, Cr-Co-Cu-As-Cd. According to ANOVA and Kruskal-Wallis test results, removal efficiencies of TSS and TOC partially affected by different temperature (p < 0.1 for TSS and p < 0.05 for TOC) and pH ranges (p < 0.1 for both removal efficiencies), TP removal efficiency significantly affected by different pH ranges (p < 0.001), and Chloride and Sulfate removal efficiencies were significantly (p < 0.001) affected by different temperature ranges. Regardless of geographical location and climatic factors, pocket wetland systems can be relied upon for minimizing heavy metals such as Cr, Co, Zn, As, Cd and Pb and critical pollutants such as TP and TSS caused by highway runoff. Copyright © 2018 Elsevier Ltd. All rights reserved.

Extreme anthropogenic erosion: Topsoil Selling in the Mekong Delta and consequences for soil quality

NASA Astrophysics Data System (ADS)

Weigand, Susanne; Sebesvari, Zita; Vien, Duong Minh; Kruse, Jens; Guong, Vo Thi; Amelung, Wulf

2017-04-01

Increasing urbanization and industrialization leads to increasing demands for construction material, especially in low income countries. For this purpose topsoil is sometimes removed and used as construction material. Topsoil Selling is practiced around the world from America, Europe and Africa to Asia. In the Mekong Delta, Vietnam farmers physically remove the upper 10-40 cm of their paddy fields and sell it to contractors (= Topsoil Selling, TSS). The excavated material is used for road construction or brick production and therefore the most fertile part of the paddy soil is irrecoverably lost. The temporal effects of topsoil removal on soil quality are not yet fully understood. We hypothesized that after soil removal, soil quality and yield potential are significantly lower compared to the original topsoil. To test this hypothesis, we sampled two chronosequences in two different provinces of the Mekong Delta. The provinces are Sóc Trăng (Control, 1, 2, 3, 8 years after TSS) and Trà Vinh (Control, 3, 5, 8 years after TSS). The sampling areas differ in texture and cultivation practice: clayey-loamy vs. sandy-loamy and double vs. triple rice cropping. For each year of the chronosequence, 4 field sites were investigated. We sampled the Ap, Bg1, and Bg2 horizon up to 40 cm depth as composite samples from 6 to 8 cores per field. Soil organic carbon (Corg) stocks at TSS sites were up to 20 t/ha lower than at Control sites (Control: 50 t/ha) in Sóc Trăng and up to 15 t/ha lower in Tra Vinh (Control: 30 t/ha). Especially the Bg horizons revealed a continuous decline in Corg with time after soil removal. Analysis of available nutrients (Na, K, Ca, Mg, S, Fe, Al, Mn, Zn, Cu) determined by the Mehlich3-Method are still ongoing. Preliminary results, however, suggest that there is not sustainable loss of these elements after selling, but that initial risk of losses are reverted under prolonged management. Phosphorus fractionation according to the Hedley method indicate, however, that easily and moderately plant available P forms are depleted, which has to be accounted for in land-restoration measures. Overall, the current data we received so far revealed that TSS induces mainly a dramatic loss of soil organic matter. It was ongoing up to the 8th year of our chronosequence; yet, it was not necessarily accompanied by losses in inorganic nutrients. As a result, there is a chance also for the farmers to overcome risks in yield decline because the former subsoils overtake the role of the former topsoils in maintaining rice production.

Effect of Hand Antisepsis Agent Selection and Population Characteristics on Surgical Site Infection Pathogens.

PubMed

Oriel, Brad S; Chen, Qi; Wong, Kevin; Itani, Kamal M F

Selection of a pre-operative hand antisepsis agent has not been studied in relation to surgical site infection (SSI) culture data. In our hospital, we introduced an alcohol-based hand rub (ABR) in 2012 as an alternative to traditional aqueous surgical scrubs (TSS). It was the goal of this study to review any effect of this implementation on SSI pathogen characteristics. In addition, we sought to compare our SSI culture data with available National Healthcare Safety Network (NHSN) data. We hypothesized that SSI pathogens and resistant isolates are affected by surgical hand antisepsis technique. Data collected prospectively between 2007 and 2014 were retrospectively analyzed for two time periods at the Veterans Affairs Boston Healthcare System (VABHS): Before ABR implementation (TSS group) and after (ABR group). Pathogen distribution and pathogenic isolate resistance profiles were compared for TSS and ABR, and similar comparisons, along with procedure-associated SSI comparisons, were made between VABHS and NHSN. All VABHS data were interpreted and categorized according to NHSN definitions. Compared with TSS (n = 4,051), ABR (n = 2,293) had a greater rate of Staphylococcus aureus (42.6% vs. 38.0%), Escherichia coli (12.8% vs. 9.9%), Pseudomonas aeruginosa (8.5% vs. 2.8%), and Enterobacter spp. (10.6% vs. 2.8%), and a lower rate of Klebsiella pneumoniae/K. oxytoca (4.3% vs. 8.5%) cultured from superficial and deep SSIs (p < 0.05). Of the S. aureus isolates, 35.0% and 44.4% were resistant to oxacillin/methicillin (MRSA) in ABR and TSS, respectively (p = 0.06). Looking at all SSIs, coagulase-negative staphylococci and K. pneumoniae/K. oxytoca at VABHS (4.0% and 10.4%, respectively) accounted for the biggest difference from NHSN (11.7% and 4.0%, respectively). Aside from MRSA, where there was no difference between VABHS and NHSN (42.9% vs. 43.7%, respectively; p = 0.87), statistically significant (p < 0.05) differences were observed among multi-drug-resistant K. pneumoniae/K. oxytoca (0% vs. 6.8%, respectively) and Escherichia coli (10.0% vs. 1.6%, respectively), as well as among extended-spectrum cephalosporin-resistant K. pneumoniae/K. oxytoca (4.8% vs. 13.2%, respectively) and Enterobacter (58.3% vs. 27.7%, respectively). VABHS had a greater proportion of SSIs in abdominal and vascular cases than did NHSN (48.6% vs. 22.5% and 13.2% vs. 1.5%, respectively). Overall, these differences were significant (p < 0.05). The TSS and ABR groups differed in the distribution of pathogens recovered. Those differences, along with SSI pathogen distribution, pathogenic isolate resistance profiles, and procedure-associated SSIs between VABHS and NHSN, warrant further investigation.

Identification of Two Loci in Tomato Reveals Distinct Mechanisms for Salt Tolerance

PubMed Central

Borsani, Omar; Cuartero, Jesus; Fernández, José A.; Valpuesta, Victoriano; Botella, Miguel A.

2001-01-01

Salt stress is one of the most serious environmental factors limiting the productivity of crop plants. To understand the molecular basis for salt responses, we used mutagenesis to identify plant genes required for salt tolerance in tomato. As a result, three tomato salt-hypersensitive (tss) mutants were isolated. These mutants defined two loci and were caused by single recessive nuclear mutations. The tss1 mutant is specifically hypersensitive to growth inhibition by Na+ or Li+ and is not hypersensitive to general osmotic stress. The tss2 mutant is hypersensitive to growth inhibition by Na+ or Li+ but, in contrast to tss1, is also hypersensitive to general osmotic stress. The TSS1 locus is necessary for K+ nutrition because tss1 mutants are unable to grow on a culture medium containing low concentrations of K+. Increased Ca2+ in the culture medium suppresses the growth defect of tss1 on low K+. Measurements of membrane potential in apical root cells were made with an intracellular microelectrode to assess the permeability of the membrane to K+ and Na+. K+-dependent membrane potential measurements indicate impaired K+ uptake in tss1 but not tss2, whereas no differences in Na+ uptake were found. The TSS2 locus may be a negative regulator of abscisic acid signaling, because tss2 is hypersensitive to growth inhibition by abscisic acid. Our results demonstrate that the TSS1 locus is essential for K+ nutrition and NaCl tolerance in tomato. Significantly, the isolation of the tss2 mutant demonstrates that abscisic acid signaling is also important for salt and osmotic tolerance in glycophytic plants. PMID:11283342

40 CFR 463.14 - New source performance standards.

Code of Federal Regulations, 2014 CFR

2014-07-01

... concentrations: Subpart A [Contact cooling and heating water] Concentration used to calculate NSPS Pollutant or pollutant property Maximum for any 1 day (mg/l) BOD5 26 Oil and grease 29 TSS 19 pH (1) 1 Within the range...

40 CFR 463.14 - New source performance standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

... concentrations: Subpart A [Contact cooling and heating water] Concentration used to calculate NSPS Pollutant or pollutant property Maximum for any 1 day (mg/l) BOD5 26 Oil and grease 29 TSS 19 pH (1) 1 Within the range...

TssA forms a gp6-like ring attached to the type VI secretion sheath.

PubMed

Planamente, Sara; Salih, Osman; Manoli, Eleni; Albesa-Jové, David; Freemont, Paul S; Filloux, Alain

2016-08-01

The type VI secretion system (T6SS) is a supra-molecular bacterial complex that resembles phage tails. It is a killing machine which fires toxins into target cells upon contraction of its TssBC sheath. Here, we show that TssA1 is a T6SS component forming dodecameric ring structures whose dimensions match those of the TssBC sheath and which can accommodate the inner Hcp tube. The TssA1 ring complex binds the T6SS sheath and impacts its behaviour in vivo In the phage, the first disc of the gp18 sheath sits on a baseplate wherein gp6 is a dodecameric ring. We found remarkable sequence and structural similarities between TssA1 and gp6 C-termini, and propose that TssA1 could be a baseplate component of the T6SS Furthermore, we identified similarities between TssK1 and gp8, the former interacting with TssA1 while the latter is found in the outer radius of the gp6 ring. These observations, combined with similarities between TssF and gp6N-terminus or TssG and gp53, lead us to propose a comparative model between the phage baseplate and the T6SS. © 2016 The Authors. Published under the terms of the CC BY 4.0 license.

Stochastic modeling of total suspended solids (TSS) in urban areas during rain events.

PubMed

Rossi, Luca; Krejci, Vladimir; Rauch, Wolfgang; Kreikenbaum, Simon; Fankhauser, Rolf; Gujer, Willi

2005-10-01

The load of total suspended solids (TSS) is one of the most important parameters for evaluating wet-weather pollution in urban sanitation systems. In fact, pollutants such as heavy metals, polycyclic aromatic hydrocarbons (PAHs), phosphorous and organic compounds are adsorbed onto these particles so that a high TSS load indicates the potential impact on the receiving waters. In this paper, a stochastic model is proposed to estimate the TSS load and its dynamics during rain events. Information on the various simulated processes was extracted from different studies of TSS in urban areas. The model thus predicts the probability of TSS loads arising from combined sewer overflows (CSOs) in combined sewer systems as well as from stormwater in separate sewer systems in addition to the amount of TSS retained in treatment devices in both sewer systems. The results of this TSS model illustrate the potential of the stochastic modeling approach for assessing environmental problems.

Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria.

PubMed

Li, Xin; Sandh, Gustaf; Nenninger, Anja; Muro-Pastor, Alicia M; Stensjö, Karin

2015-03-01

In cyanobacteria, DNA-binding proteins from starved cells (Dps) play an important role in the cellular response to oxidative and nutritional stresses. In this study, we have characterized the cell-type specificity and the promoter regions of two orthologous dps genes, Npun_R5799 in Nostoc punctiforme and alr3808 in Anabaena sp. PCC 7120. A transcriptional start site (TSS), identical in location to the previously identified proximal TSS of alr3808, was identified for Npun_R5799 under both combined nitrogen supplemented and N2-fixing growth conditions. However, only alr3808 was also transcribed from a second distal TSS. Sequence homologies suggest that the promoter region containing the distal TSS is not conserved upstream of orthologous genes among heterocyst-forming cyanobacteria. The analysis of promoter GFP-reporter strains showed a different role in governing cell-type specificity between the proximal and distal promoter of alr3808. We here confirmed the heterocyst specificity of the distal promoter of alr3808 and described a very early induction of its expression during proheterocyst differentiation. In contrast, the complete promoters of both genes were active in all cells. Even though Npun_R5799 and alr3808 are orthologs, the regulation of their respective expression differs, indicating distinctions in the function of these cyanobacterial Dps proteins depending on the strain and cell type. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Surgical Outcome of Endoscopic Endonasal Surgery for Non-Functional Pituitary Adenoma by a Team of Neurosurgeons and Otolaryngologists Adenoma by a Team of Neurosurgeons and Otolaryngologists.

PubMed

Kikuchi, Ryogo; Toda, Masahiro; Tomita, Toshiki; Ogawa, Kaoru; Yoshida, Kazunari

2017-01-01

This study aimed to assess the efficacy of endoscopic endonasal surgery, conducted by a team of neurosurgeons and otolaryngologists. We studied 40 patients who were undergoing surgery for primary non-functional pituitary adenomas with Knosp grades 1 to 3, at Keio University Hospital between 2005 and 2012. We compared the endoscopic endonasal transsphenoidal approach (team-eTSS; T-eTSS), with a microscopic transsphenoidal approach (mTSS). Analyses were conducted for differences between the two groups in tumor resection rates, operating durations, and complications from the non-functional pituitary adenomas. We also compared the heminostril and binostril approaches for T-eTSS. Tumor resection rates were higher when the surgeries were conducted by T-eTSS than mTSS. In particular, when the maximum tumor diameter was more than 25 mm, resection rates were significantly higher for T-eTSS than for mTSS. There were no unexpected complications in either group. There was no significant difference in resection rates between the heminostril and binostril approaches when T-eTSS was performed. T-eTSS is an efficacious surgical option for non-functional pituitary adenomas, particularly when the adenoma is of large size. Benefits of the heminostril approach are evident.

Denitrifying bioreactor clogging potential during wastewater treatment.

PubMed

Christianson, Laura E; Lepine, Christine; Sharrer, Kata L; Summerfelt, Steven T

2016-11-15

Chemoheterotrophic denitrification technologies using woodchips as a solid carbon source (i.e., woodchip bioreactors) have been widely trialed for treatment of diffuse-source agricultural nitrogen pollution. There is growing interest in the use of this simple, relatively low-cost biological wastewater treatment option in waters with relatively higher total suspended solids (TSS) and chemical oxygen demand (COD) such as aquaculture wastewater. This work: (1) evaluated hydraulic retention time (HRT) impacts on COD/TSS removal, and (2) assessed the potential for woodchip clogging under this wastewater chemistry. Four pilot-scale woodchip denitrification bioreactors operated for 267 d showed excellent TSS removal (>90%) which occurred primarily near the inlet, and that COD removal was maximized at lower HRTs (e.g., 56% removal efficiency and 25 g of COD removed per m 3 of bioreactor per d at a 24 h HRT). However, influent wastewater took progressively longer to move into the woodchips likely due to a combination of (1) woodchip settling, (2) clogging due to removed wastewater solids and/or accumulated bacterial growth, and (3) the pulsed flow system pushing the chips away from the inlet. The bioreactor that received the highest loading rate experienced the most altered hydraulics. Statistically significant increases in woodchip P content over time in woodchip bags placed near the bioreactor outlets (0.03 vs 0.10%P 2 O 5 ) and along the bioreactor floor (0.04 vs. 0.12%P 2 O 5 ) confirmed wastewater solids were being removed and may pose a concern for subsequent nutrient mineralization and release. Nevertheless, the excellent nitrate-nitrogen and TSS removal along with notable COD removal indicated woodchip bioreactors are a viable water treatment technology for these types of wastewaters given they are used downstream of a filtration device. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Development of an indicator for characterizing particle size distribution and quality of stormwater runoff.

PubMed

Wang, Qian; Zhang, Qionghua; Dzakpasu, Mawuli; Lian, Bin; Wu, Yaketon; Wang, Xiaochang C

2018-03-01

Stormwater particles washed from road-deposited sediments (RDS) are traditionally characterized as either turbidity or total suspended solids (TSS). Although these parameters are influenced by particle sizes, neither of them characterizes the particle size distribution (PSD), which is of great importance in pollutant entrainment and treatment performance. Therefore, the ratio of turbidity to TSS (Tur/TSS) is proposed and validated as a potential surrogate for the bulk PSD and quality of stormwater runoff. The results show an increasing trend of Tur/TSS with finer sizes of both RDS and stormwater runoff. Taking heavy metals (HMs, including Cu, Pb, Zn, Cr, and Ni) as typical pollutants in stormwater runoff, the concentrations (mg/kg) were found to vary significantly during rainfall events and tended to increase significantly with Tur/TSS. Therefore, Tur/TSS is a valid parameter to characterize the PSD and quality of stormwater. The high negative correlations between Tur/TSS and rainfall intensity demonstrate that stormwater with higher Tur/TSS generates under low intensity and, thus, characterizes small volume, finer sizes, weak settleability, greater mobility, and bioavailability. Conversely, stormwater with lower Tur/TSS generates under high intensity and, thus, characterizes large volume, coarser sizes, good settleability, low mobility, and bioavailability. These results highlight the need to control stormwater with high Tur/TSS. Moreover, Tur/TSS can aid the selection of stormwater control measures with appropriate detention storage, pollution loading, and removal effectiveness of particles.

Origins, seasonality, and fluxes of organic matter in the Congo River

NASA Astrophysics Data System (ADS)

Spencer, Robert G. M.; Hernes, Peter J.; Dinga, Bienvenu; Wabakanghanzi, Jose N.; Drake, Travis W.; Six, Johan

2016-07-01

The Congo River in central Africa represents a major source of organic matter (OM) to the Atlantic Ocean. This study examined elemental (%OC, %N, and C:N), stable isotopic (δ13C and δ15N), and biomarker composition (lignin phenols) of particulate OM (POM) and dissolved OM (DOM) across the seasonal hydrograph. Even though the Congo exhibits an extremely stable intra-annual discharge regime, seasonal variability in OM composition was evident. DOM appears predominantly derived from vascular plant inputs with greater relative contribution during the rising limb and peak in discharge associated with the major November-December discharge maximum. Generally, POM appears to be sourced from soil-derived mineral-associated OM (low C:N, low Λ8, and higher (Ad:Al)v) but the relative proportion of fresh vascular plant material (higher C:N, higher Λ8, and lower (Ad:Al)v) increases with higher discharge. During the study period (September 2009 to November 2010) the Congo exported 29.21 Tg yr-1 of total suspended sediment (TSS), 1.96 Tg yr-1 of particulate organic carbon (POC), and 12.48 Tg yr-1 of dissolved organic carbon. The Congo exports an order of magnitude lower TSS load in comparison to other major riverine sources of TSS (e.g., Ganges and Brahmaputra), but due to its OM-rich character it actually exports a comparable amount of POC. The Congo is also 2.5 times more efficient at exporting dissolved lignin per unit volume compared to the Amazon. Including Congo dissolved lignin data in residence time calculations for lignin in the Atlantic Ocean results in an approximately 10% reduction from the existing estimate, suggesting that this material is more reactive than previously thought.

40 CFR 406.15 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Wet Milling Subcategory § 406.15... Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 1.08 0.36 TSS 1.35 .45 pH (1) (1) English units (pounds per 1,000 stdbu...

40 CFR 406.25 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling Subcategory § 406.25... Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 0.11 0.036 TSS 0.054 0.18 pH (1) (1) English units (pounds per 1,000...

40 CFR 406.25 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling Subcategory § 406.25... Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 0.11 0.036 TSS 0.054 0.18 pH (1) (1) English units (pounds per 1,000...

40 CFR 406.25 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling Subcategory § 406.25... Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 0.11 0.036 TSS 0.054 0.18 pH (1) (1) English units (pounds per 1,000...

40 CFR 406.15 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Wet Milling Subcategory § 406.15... Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 1.08 0.36 TSS 1.35 .45 pH (1) (1) English units (pounds per 1,000 stdbu...

40 CFR 406.15 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Wet Milling Subcategory § 406.15... Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 1.08 0.36 TSS 1.35 .45 pH (1) (1) English units (pounds per 1,000 stdbu...

The Aplication of Landsat 8 OLI for Total Suspended Solid (TSS) Mapping in Gajahmungkur Reservoir Wonogiri Regency 2016

NASA Astrophysics Data System (ADS)

Yanti, Apriwida; Susilo, Bowo; Wicaksono, Pramaditya

2016-11-01

Gajahmungkur reservoir is administratively located in Wonogiri Regency, Central Java, with the main function as a flood control in the upstream of Bengawan Solo River. Other functions of the reservoir are as hydroelectric power plant (PLTA), water supply, irrigation, fisheries and tourism. Economic utilization of the reservoir is estimated until 100 years, but it is begun to be threatened by the silting of the reservoir. Eroded materials entering water body will be suspended and accumulated. Suspended Material or TSS (Total Suspended Solid) will increase the turbidity of water, which can affect the quality of water and silting the reservoir. Remote sensing technology can be used to determine the spatial distribution of TSS. The purposes of this study were to 1) utilize and compare the accuracy of single band Landsat 8 OLI for mapping the spatial distribution of TSS and 2) estimate the TSS on Gajahmungkur reservoir surface waters up to the depth of 30 cm. The method used for modelling the TSS spatial distribution is the empirical modelling that integrates image pixel values and field data using correlation analysis and regression analysis. The data used in the empirical modelling are single band of visible, NIR, and SWIR of Landsat 8 OLI, which was acquired on 8 May 2016, and field-measured TSS values based on the field data collection conducted on 12 April 2016. The results revealed that mapping the distribution and the estimated value of TSS in Reservoir Gajahmungkur can be performed more accurately using band 4 (red band). The determinant coefficient between TSS field and TSS value of image using band 4 is 0.5431. The Standard Error (SE) of the predicted TSS value is 16.16 mg/L. The results also showed that the estimated total TSS of May 2016 according to band 4 is 1.087,56 tons. The average estimation of TSS value in up to the depth of 30 cm is 61.61 mg/L. The highest TSS distribution is in the northern parts, which was dominated by eroded materials from Keduang River.

Gross total resection of pituitary adenomas after endoscopic vs. microscopic transsphenoidal surgery: a meta-analysis.

PubMed

Almutairi, Reem D; Muskens, Ivo S; Cote, David J; Dijkman, Mark D; Kavouridis, Vasileios K; Crocker, Erin; Ghazawi, Kholoud; Broekman, Marike L D; Smith, Timothy R; Mekary, Rania A; Zaidi, Hasan A

2018-05-01

Microscopic transsphenoidal surgery (mTSS) is a well-established method to address adenomas of the pituitary gland. Endoscopic transsphenoidal surgery (eTSS) has become a viable alternative, however. Advocates suggest that the greater illumination, panoramic visualization, and angled endoscopic views afforded by eTSS may allow for higher rates of gross total tumor resection (GTR). The aim of this meta-analysis was to determine the rate of GTR using mTSS and eTSS. A meta-analysis of the literature was conducted using PubMed, EMBASE, and Cochrane databases through July 2017 in accordance with PRISMA guidelines. Seventy case series that reported GTR rate in 8257 pituitary adenoma patients were identified. For all pituitary adenomas, eTSS (GTR=74.0%; I 2  = 92.1%) was associated with higher GTR as compared to mTSS (GTR=66.4%; I 2  = 84.0%) in a fixed-effect model (P-interaction < 0.01). For functioning pituitary adenomas (FPAs) (n = 1170 patients), there was no significant difference in GTR rate between eTSS (GTR=75.8%; I 2  = 63.9%) and mTSS (GTR=75.5%; I 2  = 79.0%); (P-interaction = 0.92). For nonfunctioning pituitary adenomas (NFPAs) (n = 2655 patients), eTSS (GTR=71.0%; I 2  = 86.4%) was associated with higher GTR as compared to mTSS (GTR=60.7%; I 2  = 87.5%) in a fixed-effect model (P-interaction < 0.01). None of the associations were significant in a random-effect model (all P-interaction > 0.05). No significant publication bias was identified for any of the outcomes. Among patients who were not randomly allocated to either approach, eTSS resulted in a higher rate of GTR as compared to mTSS for all patients and for NFPA patients alone, but only in a fixed-effect model. For FPA, however, eTSS did not seem to offer a significantly higher rate of GTR. These conclusions should be interpreted with caution because of the nature of the included non-comparative studies.

Municipal Leachate Treatment by Fenton Process: Effect of Some Variable and Kinetics

PubMed Central

Ahmadian, Mohammad; Reshadat, Sohyla; Yousefi, Nader; Mirhossieni, Seyed Hamed; Zare, Mohammad Reza; Ghasemi, Seyed Ramin; Rajabi Gilan, Nader; Khamutian, Razieh; Fatehizadeh, Ali

2013-01-01

Due to complex composition of leachate, the comprehensive leachate treatment methods have been not demonstrated. Moreover, the improper management of leachate can lead to many environmental problems. The aim of this study was application of Fenton process for decreasing the major pollutants of landfill leachate on Kermanshah city. The leachate was collected from Kermanshah landfill site and treated by Fenton process. The effect of various parameters including solution pH, Fe2+ and H2O2 dosage, Fe2+/H2O2 molar ratio, and reaction time was investigated. The result showed that with increasing Fe2+ and H2O2 dosage, Fe2+/H2O2 molar ratio, and reaction time, the COD, TOC, TSS, and color removal increased. The maximum COD, TOC, TSS, and color removal were obtained at low pH (pH: 3). The kinetic data were analyzed in term of zero-order, first-order, and second-order expressions. First-order kinetic model described the removal of COD, TOC, TSS, and color from leachate better than two other kinetic models. In spite of extremely difficulty of leachate treatment, the previous results seem rather encouraging on the application of Fenton's oxidation. PMID:23840229

An evaluation of the urban stormwater pollutant removal efficiency of catch basin inserts.

PubMed

Morgan, Robert A; Edwards, Findlay G; Brye, Kristofor R; Burian, Stephen J

2005-01-01

In a storm sewer system, the catch basin is the interface between surface runoff and the sewer. Responding to the need to improve the quality of stormwater from urban areas and transportation facilities, and spurred by Phase I and II Stormwater Rules from the U.S. Environmental Protection Agency, several companies market catch basin inserts as best management practices for urban water quality management. However, little data have been collected under controlled tests that indicate the pollutant removal efficiency of these inserts when the inflow is near what can be expected to occur in the field. A stormwater simulator was constructed to test inserts under controlled and replicable conditions. The inserts were tested for removal efficiency of total suspended solids (TSS) and total petroleum hydrocarbons (TPH) at an inflow rate of 757 to 814 L/min, with influent pollutant concentrations of 225 mg/L TSS and 30 mg/L TPH. These conditions are similar to stormwater runoff from small commercial sites in the southeastern United States. Results from the tests indicate that at the test flowrate and pollutant concentration, average TSS removal efficiencies ranged from 11 to 42% and, for TPH, the removal efficiency ranged from 10 to 19%.

Comprehensive comparative analysis of 5'-end RNA-sequencing methods.

PubMed

Adiconis, Xian; Haber, Adam L; Simmons, Sean K; Levy Moonshine, Ami; Ji, Zhe; Busby, Michele A; Shi, Xi; Jacques, Justin; Lancaster, Madeline A; Pan, Jen Q; Regev, Aviv; Levin, Joshua Z

2018-06-04

Specialized RNA-seq methods are required to identify the 5' ends of transcripts, which are critical for studies of gene regulation, but these methods have not been systematically benchmarked. We directly compared six such methods, including the performance of five methods on a single human cellular RNA sample and a new spike-in RNA assay that helps circumvent challenges resulting from uncertainties in annotation and RNA processing. We found that the 'cap analysis of gene expression' (CAGE) method performed best for mRNA and that most of its unannotated peaks were supported by evidence from other genomic methods. We applied CAGE to eight brain-related samples and determined sample-specific transcription start site (TSS) usage, as well as a transcriptome-wide shift in TSS usage between fetal and adult brain.

Load limit of a UASB fed septic tank-treated domestic wastewater.

PubMed

Lohani, Sunil Prasad; Bakke, Rune; Khanal, Sanjay N

2015-01-01

Performance of a 250 L pilot-scale up-flow anaerobic sludge blanket (UASB) reactor, operated at ambient temperatures, fed septic tank effluents intermittently, was monitored for hydraulic retention time (HRT) from 18 h to 4 h. The total suspended solids (TSS), total chemical oxygen demand (CODT), dissolved chemical oxygen demand (CODdis) and suspended chemical oxygen demand (CODss) removal efficiencies ranged from 20 to 63%, 15 to 56%, 8 to 35% and 22 to 72%, respectively, for the HRT range tested. Above 60% TSS and 47% CODT removal were obtained in the combined septic tank and UASB process. The process established stable UASB treatment at HRT≥6 h, indicating a hydraulic load design limit. The tested septic tank-UASB combined system can be a low-cost and effective on-site sanitation solution.

A Landsat-based model for retrieving total suspended solids concentration of estuaries and coasts in China

NASA Astrophysics Data System (ADS)

Wang, Chongyang; Chen, Shuisen; Li, Dan; Wang, Danni; Liu, Wei; Yang, Ji

2017-11-01

Retrieving total suspended solids (TSS) concentration accurately is essential for sustainable management of estuaries and coasts, which plays a key role in the interaction between hydrosphere, pedosphere and atmosphere. Although many TSS retrieval models have been published, the general inversion method that is applicable to different field conditions is still under research. In order to obtain a TSS remote sensing model that is suitable for estimating TSS concentrations with wide range in estuaries and coasts by Landsat imagery, after reviewing a number of Landsat-based TSS retrieval models and improving a comparatively better one among them, this study developed a quadratic model using the ratio of logarithmic transformation of red band and near-infrared band and logarithmic transformation of TSS concentration (QRLTSS) based on 119 in situ samples collected in 2006-2013 from five regions of China. It was found that the QRLTSS model works well and shows a satisfactory performance. The QRLTSS model based on Landsat TM (Thematic Mapper), ETM+ (Enhanced Thematic Mapper Plus) and OLI (Operational Land Imager) sensors explained about 72 % of the TSS concentration variation (TSS: 4.3-577.2 mg L-1, N = 84, P value < 0.001) and had an acceptable validation accuracy (TSS: 4.5-474 mg L-1, root mean squared error (RMSE) ≤ 25 mg L-1, N = 35). In addition, a threshold method of red-band reflectance (OLI: 0.032, ETM+ and TM: 0.031) was proposed to solve the two-valued issue of the QRLTSS model and to retrieve TSS concentration from Landsat imagery. After a 6S model-based atmospheric correction of Landsat OLI and ETM+ imagery, the TSS concentrations of three regions (Moyangjiang River estuary, Pearl River estuary and Hanjiang River estuary) in Guangdong Province in China were mapped by the QRLTSS model. The results indicated that TSS concentrations in the three estuaries showed large variation ranging from 0.295 to 370.4 mg L-1. Meanwhile we found that TSS concentrations retrieved from Landsat imagery showed good validation accuracies with the synchronous water samples (TSS: 7-160 mg L-1, RMSE: 11.06 mg L-1, N = 22). The further validation from EO-1 Hyperion imagery also showed good performance (in situ synchronous measurement of TSS: 106-220.7 mg L-1, RMSE: 26.66 mg L-1, N = 13) of the QRLTSS model for the area of high TSS concentrations in the Lingding Bay of the Pearl River estuary. Evidently, the QRLTSS model is potentially applied to simulate high-dynamic TSS concentrations of other estuaries and coasts by Landsat imagery, improving the understanding of the spatial and temporal variation of TSS concentrations on regional and global scales. Furthermore, the QRLTSS model can be optimized to establish a regional or unified TSS retrieval model of estuaries and coasts in the world for different satellite sensors with medium- and high-resolution similar to Landsat TM, ETM+ and OLI sensors or with similar red bands and near-infrared bands, such as ALI, HJ-1 A and B, LISS, CBERS, ASTER, ALOS, RapidEye, Kanopus-V, and GF.

Scanning Laser Polarimetry with Variable and Enhanced Corneal Compensation in Normal and Glaucomatous Eyes

PubMed Central

Sehi, Mitra; Guaqueta, Delia C.; Feuer, William J.; Greenfield, David S.

2007-01-01

Purpose To investigate whether correction for atypical birefringence pattern (ABP) using scanning laser polarimetry with enhanced corneal compensation (SLP-ECC) reduces the severity of ABP compared with variable corneal compensation (SLP-VCC), and improves the correlation with visual function. Design Prospective observational study. Methods Normal and glaucomatous eyes enrolled from 4 clinical sites underwent complete examination, automated perimetry, SLP-ECC and SLP-VCC. Eyes were characterized in 3 groups based upon the typical scan score (TSS): normal birefringence pattern (NBP) was defined as TSS 80, mild ABP as TSS 61–79, moderate-severe ABP as TSS 60. For each of 4 SLP parameters the area under the ROC curve (AUROC) was calculated to compare the ability of SLP-ECC and SLP-VCC to discriminate between normal and glaucomatous eyes. Results Eighty-four normal volunteers and 45 glaucoma patients were enrolled. Mean TSS was significantly (p<0.001) greater using SLP-ECC (98.0 ± 6.6) compared to SLP-VCC (83.4 ± 22.5). The frequency of moderate-severe ABP images was significantly (p<0.001, McNemar test) higher using SLP-VCC (18 of 129, 14%) compared to SLP-ECC (1 of 129, 0.8%). Two SLP-ECC parameters (TSNIT average and inferior average) had significantly (p=0.01, p<0.001) higher correlation (r=0.45, r=0.50 respectively) with MD compared to SLP-VCC (r=0.34, r=0.37). Among eyes with moderate-severe ABP (N = 18), inferior average obtained using SLP-ECC had significantly (p=0.03) greater AUROC (0.91 ± 0.07) compared with SLP-VCC (0.78 ± 0.11). Conclusions SLP-ECC significantly reduces the frequency and severity of ABP compared to SLP-VCC and improves the correlation between RNFL measures and visual function. PMID:17157800

Evaluating suitability of MODIS-Terra images for reproducing historic sediment concentrations in water bodies: Lake Tana, Ethiopia

NASA Astrophysics Data System (ADS)

Kaba, Essayas; Philpot, William; Steenhuis, Tammo

2014-02-01

Government and NGO funded conservation programs are being implemented in developing countries with the potential benefit of reduced sediment inflow into fresh water lakes. However, these claims are difficult to verify due to limited historical sediment concentration data in lakes and rivers. Remote sensing can potentially aid in monitoring sediment concentration. With almost daily availability over the past ten years and consistent atmospheric correction applied to the images, Moderate Resolution Imaging Spectroradiometer (MODIS) 250 meter images are potential resources capable of monitoring future concentrations and reconstructing historical sediment concentration records. In this paper, site-specific relationships are developed between reflectance in near-infrared (NIR) images and three factors: total suspended solids (TSS), turbidity and Secchi depth for Lake Tana near the mouth of the Gumara River. The first two sampling campaigns on November 27, 2010 and May 13, 2011 are used in calibration. Reflectance in the NIR varies linearly with turbidity (R2 = 0.89) and TSS (R2 = 0.95). Secchi depth fit best to an exponential relation with R2 of 0.74. The relationships are validated using a third sample set collected on November 7, 2011 with RMSE of 11 Nephelometric Turbidity Units (NTU) for Turbidity, 16.5 mg l-1 for TSS and 0.12 meters for Secchi depth. The MAE was 10% for TSS, 14% for turbidity and 0.1% for Secchi depth. Using the relationship for TSS, a 10-year time series of sediment concentration in Lake Tana near the Gumara River was plotted. It was found that after the severe drought of 2002 and 2003 the concentration in the lake increased significantly. The results showed that MODIS images are potential cost effective tools to monitor suspended sediment concentration and obtain a past history of concentration for evaluating the effect of best management practices.

40 CFR 411.15 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... exceed 3 °C rise above inlet temperature. pH Within the range 6.0 to 9.0. English units (lb/1,000 lb of product) TSS 0.005. Temperature (heat) Not to exceed 3 °C rise above inlet temperature. pH Within the...

40 CFR 408.275 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... steamed and canned oyster processing facilities which utilize air flotation treatment systems to meet the....42 pH (1) (1) English units (lb/1,000 lb of product) BOD5 67 17 TSS 56 39 Oil and grease 0.64 0.42 pH...

40 CFR 471.53 - New source performance standards (NSPS).

Code of Federal Regulations, 2014 CFR

2014-07-01

... TSS 123 97.9 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (p) Molten salt rinse. Subpart... (pounds per million off-pounds) of refractory metals treated with molten salt Copper 0.810 0.386 Nickel 0...

40 CFR 471.53 - New source performance standards (NSPS).

Code of Federal Regulations, 2012 CFR

2012-07-01

... TSS 123 97.9 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (p) Molten salt rinse. Subpart... (pounds per million off-pounds) of refractory metals treated with molten salt Copper 0.810 0.386 Nickel 0...

40 CFR 428.32 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS RUBBER MANUFACTURING POINT SOURCE CATEGORY Solution...) (1) English units (lb/1,000 lb of product) COD 5.91 3.94 BOD5 0.60 .40 TSS 0.98 .65 Oil and grease 0...

40 CFR 411.15 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... exceed 3 °C rise above inlet temperature. pH Within the range 6.0 to 9.0. English units (lb/1,000 lb of product) TSS 0.005. Temperature (heat) Not to exceed 3 °C rise above inlet temperature. pH Within the...

Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages.

PubMed

Shanks, John; Burtnick, Mary N; Brett, Paul J; Waag, David M; Spurgers, Kevin B; Ribot, Wilson J; Schell, Mark A; Panchal, Rekha G; Gherardini, Frank C; Wilkinson, Keith D; Deshazer, David

2009-04-01

Burkholderia mallei, a category B biothreat agent, is a facultative intracellular pathogen that causes the zoonotic disease glanders. The B. mallei VirAG two-component regulatory system activates the transcription of approximately 60 genes, including a large virulence gene cluster encoding a type VI secretion system (T6SS). The B. mallei tssM gene encodes a putative ubiquitin-specific protease that is physically linked to, and transcriptionally coregulated with, the T6SS gene cluster. Mass spectrometry and immunoblot analysis demonstrated that TssM was secreted in a virAG-dependent manner in vitro. Surprisingly, the T6SS was found to be dispensable for the secretion of TssM. The C-terminal half of TssM, which contains Cys and His box motifs conserved in eukaryotic deubiquitinases, was purified and biochemically characterized. Recombinant TssM hydrolyzed multiple ubiquitinated substrates and the cysteine at position 102 was critical for enzymatic activity. The tssM gene was expressed within 1 h after uptake of B. mallei into RAW 264.7 murine macrophages, suggesting that the TssM deubiquitinase is produced in this intracellular niche. Although the physiological substrate(s) is currently unknown, the TssM deubiquitinase may provide B. mallei a selective advantage in the intracellular environment during infection.

The Impact of Retardance Pattern Variability on Nerve Fiber Layer Measurements over Time Using GDx with Variable and Enhanced Corneal Compensation

PubMed Central

Grewal, Dilraj S.; Sehi, Mitra; Cook, Richard J.

2011-01-01

Purpose. To examine the impact of retardance pattern variability on retinal nerve fiber layer (RNFL) measurements over time using scanning laser polarimetry with variable (GDxVCC) and enhanced corneal compensation (GDxECC; both by Carl Zeiss Meditec, Inc., Dublin, CA). Methods. Glaucoma suspect and glaucomatous eyes with 4 years of follow-up participating in the Advanced Imaging in Glaucoma Study were prospectively enrolled. All eyes underwent standard automated perimetry (SAP), GDxVCC, and GDxECC imaging every 6 months. SAP progression was determined with point-wise linear regression analysis of SAP sensitivity values. Typical scan score (TSS) values were extracted as a measure of retardance image quality; an atypical retardation pattern (ARP) was defined as TSS < 80. TSS fluctuation over time was measured using three parameters: change in TSS from baseline, absolute difference (maximum minus minimum TSS value), and TSS variance. Linear mixed-effects models that accommodated the association between the two eyes were constructed to evaluate the relationship between change in TSS and RNFL thickness over time. Results. Eighty-six eyes (51 suspected glaucoma, 35 glaucomatous) of 45 patients were enrolled. Twenty (23.3%) eyes demonstrated SAP progression. There was significantly greater fluctuation in TSS over time with GDxVCC compared with GDxECC as measured by absolute difference (18.40 ± 15.35 units vs. 2.50 ± 4.69 units; P < 0.001), TSS variance (59.63 ± 87.27 units vs. 3.82 ± 9.63 units, P < 0.001), and change in TSS from baseline (−0.83 ± 11.2 vs. 0.25 ± 2.9, P = 0.01). The change in TSS over time significantly (P = 0.006) influenced the TSNIT average RNFL thickness when measured by GDxVCC but not by GDxECC. Conclusions. Longitudinal images obtained with GDxECC have significantly less variability in TSS and retardance patterns and have reduced bias produced by ARP on RNFL progression assessment. PMID:21296821

40 CFR 434.22 - Effluent limitation guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... consecutive days Concentration in mg/l Iron, total 7.0 3.5 Manganese, total 4.0 2.0 TSS 70 35 pH 1 1 1 Within... if discharges from such point sources normally exhibit a pH of less than 6.0 prior to treatment: BPT... practicable control technology currently available if discharges from such point sources normally exhibit a pH...

40 CFR 434.22 - Effluent limitation guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... consecutive days Concentration in mg/l Iron, total 7.0 3.5 Manganese, total 4.0 2.0 TSS 70 35 pH 1 1 1 Within... if discharges from such point sources normally exhibit a pH of less than 6.0 prior to treatment: BPT... practicable control technology currently available if discharges from such point sources normally exhibit a pH...

40 CFR 434.22 - Effluent limitation guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... consecutive days Concentration in mg/l Iron, total 7.0 3.5 Manganese, total 4.0 2.0 TSS 70 35 pH 1 1 1 Within... if discharges from such point sources normally exhibit a pH of less than 6.0 prior to treatment: BPT... practicable control technology currently available if discharges from such point sources normally exhibit a pH...

40 CFR 434.22 - Effluent limitation guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... consecutive days Concentration in mg/l Iron, total 7.0 3.5 Manganese, total 4.0 2.0 TSS 70 35 pH 1 1 1 Within... if discharges from such point sources normally exhibit a pH of less than 6.0 prior to treatment: BPT... practicable control technology currently available if discharges from such point sources normally exhibit a pH...

40 CFR 434.22 - Effluent limitation guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... consecutive days Concentration in mg/l Iron, total 7.0 3.5 Manganese, total 4.0 2.0 TSS 70 35 pH 1 1 1 Within... if discharges from such point sources normally exhibit a pH of less than 6.0 prior to treatment: BPT... practicable control technology currently available if discharges from such point sources normally exhibit a pH...

The spatial and temporal variation of total suspended solid concentration in Pearl River Estuary during 1987-2015 based on remote sensing.

PubMed

Wang, Chongyang; Li, Weijiao; Chen, Shuisen; Li, Dan; Wang, Danni; Liu, Jia

2018-03-15

The movement and migration of total suspended solid (TSS) are the essential component of global material cycling and change. Based on the TSS concentrations retrieved from 112 scenes of Landsat remote sensing imageries during 1987-2015, the spatial and temporal variations of TSS concentration in high flow season and low flow seasons of six sub-regions (west shoal, west channel, middle shoal, east channel, east shoal and Pearl River Estuary Chinese White Dolphin National Nature Reserve and its adjacent waters (NNR)) of Pearl River Estuary (PRE) were analyzed and compared by statistical simulation. It was found that TSS concentrations in east and west shoals were about 23mg/L and 64mg/L higher than that of the middle shoal, respectively. There was a significant decreasing trend of TSS concentration from the northwest (223.7mg/L) to southeast (51.4mg/L) of study area, with an average reduction of 5.86mg/Lperkm, which mainly attributes to unique interaction of runoff and tide in PRE. In high flow season, there existed a significant and definite annual cycle period (5-8years) of TSS concentration change primarily responding to the periodic variation of precipitation. There were five full-fledged period changes of TSS detected in west shoal and west channel (the years of changes in 1988, 1994, 1998, 2003, 2010, 2015), while there were the last four cycle periods found in middle shoal, east channel, east shoal and NNR only. TSS concentrations in shoals and channels of PRE showed a significant decreased trend mainly due to the dam construction at the same time, with an average annual TSS concentration decrease of 5.7-10.1mg/L in high flow season from 1988 to 2015. There was no significant change trend of TSS concentration in NNR before 2003, but the TSS concentration decreased significantly after the establishment of the NNR since June 2003, with an average annual decrease of 9.7mg/L from 2004 to 2015. It was deduced that man-made protection measures had a great influence on the variation trend and intensity of TSS concentration in PRE, but had little effect on the cycle of TSS changes, indicating that the cyclical change is a very strong natural law. In low flow season, there was no significant change trend of TSS concentrations in PRE except that TSS concentrations in west channel and middle shoal showed a weak increasing trend (2.1mg/L and 2.9mg/L, respectively), which is probably because of controlled discharge for avoiding the intrusion of saltwater in PRE. Evidently, the change trend and cycle periods of TSS concentration in high- and low-flow seasons in six sub-regions of PRE had significant difference. The decreasing trend and cycle periods of TSS concentration mainly occurred in high flow season. The change trend and cycle periods of TSS concentration in low flow season was relatively small in PRE. The study shows that long series mapping of Landsat remote sensing images is an effective way to help understanding the spatial and temporal variation of TSS concentrations of estuaries and coasts, and to increase awareness of environmental change and human activity effects. Copyright © 2017 Elsevier B.V. All rights reserved.

Enhancers Are Major Targets for Murine Leukemia Virus Vector Integration

PubMed Central

De Ravin, Suk See; Su, Ling; Theobald, Narda; Choi, Uimook; Macpherson, Janet L.; Poidinger, Michael; Symonds, Geoff; Pond, Susan M.; Ferris, Andrea L.; Hughes, Stephen H.

2014-01-01

ABSTRACT Retroviral vectors have been used in successful gene therapies. However, in some patients, insertional mutagenesis led to leukemia or myelodysplasia. Both the strong promoter/enhancer elements in the long terminal repeats (LTRs) of murine leukemia virus (MLV)-based vectors and the vector-specific integration site preferences played an important role in these adverse clinical events. MLV integration is known to prefer regions in or near transcription start sites (TSS). Recently, BET family proteins were shown to be the major cellular proteins responsible for targeting MLV integration. Although MLV integration sites are significantly enriched at TSS, only a small fraction of the MLV integration sites (<15%) occur in this region. To resolve this apparent discrepancy, we created a high-resolution genome-wide integration map of more than one million integration sites from CD34+ hematopoietic stem cells transduced with a clinically relevant MLV-based vector. The integration sites form ∼60,000 tight clusters. These clusters comprise ∼1.9% of the genome. The vast majority (87%) of the integration sites are located within histone H3K4me1 islands, a hallmark of enhancers. The majority of these clusters also have H3K27ac histone modifications, which mark active enhancers. The enhancers of some oncogenes, including LMO2, are highly preferred targets for integration without in vivo selection. IMPORTANCE We show that active enhancer regions are the major targets for MLV integration; this means that MLV preferentially integrates in regions that are favorable for viral gene expression in a variety of cell types. The results provide insights for MLV integration target site selection and also explain the high risk of insertional mutagenesis that is associated with gene therapy trials using MLV vectors. PMID:24501411

On-site phytoremediation applicability assessment in Alur Ilmu, Universiti Kebangsaan Malaysia based on spatial and pollution removal analyses.

PubMed

Mahmud, Mohd Hafiyyan; Lee, Khai Ern; Goh, Thian Lai

2017-10-01

The present paper aims to assess the phytoremediation performance based on pollution removal efficiency of the highly polluted region of Alur Ilmu urban river for its applicability of on-site treatment. Thirteen stations along Alur Ilmu were selected to produce thematic maps through spatial distribution analysis based on six water quality parameters of Malaysia's Water Quality Index (WQI) for dry and raining seasons. The maps generated were used to identify the highly polluted region for phytoremediation applicability assessment. Four free-floating plants were tested in treating water samples from the highly polluted region under three different conditions, namely controlled, aerated and normal treatments. The selected free-floating plants were water hyacinth (Eichhornia crassipes), water lettuce (Pistia stratiotes), rose water lettuce (Pistia sp.) and pennywort (Centella asiatica). The results showed that Alur Ilmu was more polluted during dry season compared to raining season based on the water quality analysis. During dry season, four parameters were marked as polluted along Alur Ilmu, namely dissolve oxygen (DO), 4.72 mg/L (class III); ammoniacal nitrogen (NH 3 -N), 0.85 mg/L (class IV); total suspended solid (TSS), 402 mg/L (class V) and biological oxygen demand (BOD), 3.89 mg/L (class III), whereas, two parameters were classed as polluted during raining season, namely total suspended solid (TSS), 571 mg/L (class V) and biological oxygen demand (BOD), 4.01 mg/L (class III). The thematic maps generated from spatial distribution analysis using Kriging gridding method showed that the highly polluted region was recorded at station AL 5. Hence, water samples were taken from this station for pollution removal analysis. All the free-floating plants were able to reduce TSS and COD in less than 14 days. However, water hyacinth showed the least detrimental effect from the phytoremediation process compared to other free-floating plants, thus made it a suitable free-floating plants to be used for on-site treatment.

Infectious shock and toxic shock syndrome diagnoses in hospitals, Colorado, USA.

PubMed

Smit, Michael A; Nyquist, Ann-Christine; Todd, James K

2013-11-01

In Colorado, USA, diagnoses coded as toxic shock syndrome (TSS) constituted 27.3% of infectious shock cases during 1993-2006. The incidence of staphylococcal TSS did not change significantly overall or in female patients 10-49 years of age but increased for streptococcal TSS. TSS may be underrecognized among all ages and both sexes.

Atmospheric verification mission for the TSS/STARFAC tethered satellite

NASA Technical Reports Server (NTRS)

Wood, George M., Jr.; Stuart, Thomas D.; Crouch, Donald S.; Deloach, Richard; Brown, Kenneth G.

1991-01-01

Two types of a tethered satellite system (TSS) - a basic 1.8-m-diameter spherical spacecraft and the Shuttle Tethered Aerothermodynamic Research Facility (STARFAC) are considered. Issues related to the deployment and retrieval of a large satellite with exceedingly long tethers are discussed, and the objectives of an Atmospheric Verification Mission (ATM) are outlined. Focus is concentrated on the ATM satellite which will fly after TSS-1 and before the fully instrumented and costlier TSS-2. The differences between the AVM and TSS-2, including the configuration of the aerodynamic stabilizers, instrumentation, and the materials of construction are outlined. The basic Kevlar tether defined for the TSS-2 is being considered for use with the AVM, however, a complex tether is under consideration as well.

Identification of thyroid hormone receptor binding sites and target genes using ChIP-on-chip in developing mouse cerebellum.

PubMed

Dong, Hongyan; Yauk, Carole L; Rowan-Carroll, Andrea; You, Seo-Hee; Zoeller, R Thomas; Lambert, Iain; Wade, Michael G

2009-01-01

Thyroid hormone (TH) is critical to normal brain development, but the mechanisms operating in this process are poorly understood. We used chromatin immunoprecipitation to enrich regions of DNA bound to thyroid receptor beta (TRbeta) of mouse cerebellum sampled on post natal day 15. Enriched target was hybridized to promoter microarrays (ChIP-on-chip) spanning -8 kb to +2 kb of the transcription start site (TSS) of 5000 genes. We identified 91 genes with TR binding sites. Roughly half of the sites were located in introns, while 30% were located within 1 kb upstream (5') of the TSS. Of these genes, 83 with known function included genes involved in apoptosis, neurodevelopment, metabolism and signal transduction. Two genes, MBP and CD44, are known to contain TREs, providing validation of the system. This is the first report of TR binding for 81 of these genes. ChIP-on-chip results were confirmed for 10 of the 13 binding fragments using ChIP-PCR. The expression of 4 novel TH target genes was found to be correlated with TH levels in hyper/hypothyroid animals providing further support for TR binding. A TRbeta binding site upstream of the coding region of myelin associated glycoprotein was demonstrated to be TH-responsive using a luciferase expression system. Motif searches did not identify any classic binding elements, indicating that not all TR binding sites conform to variations of the classic form. These findings provide mechanistic insight into impaired neurodevelopment resulting from TH deficiency and a rich bioinformatics resource for developing a better understanding of TR binding.

Screening and evaluation of polymers as flocculation aids for the treatment of aquacultural effluents

USGS Publications Warehouse

Ebeling, J.M.; Rishel, K.L.; Sibrell, P.L.

2005-01-01

As environmental regulations become more stringent, environmentally sound waste management and disposal are becoming increasingly more important in all aquaculture operations. One of the primary water quality parameters of concern is the suspended solids concentration in the discharged effluent. For example, EPA initially considered the establishment of numerical limitations for only one single pollutant: total suspended solids (TSS). For recirculation systems, the proposed TSS limitations would have applied to solids polishing or secondary solids removal technology. The new rules and regulations from EPA (August 23, 2004) require only qualitative TSS limits, in the form of solids control best management practices (BMP), allowing individual regional and site specific conditions to be addressed by existing state or regional programs through NPDES permits. In recirculation systems, microscreen filters are commonly used to remove the suspended solids from the process water. Further concentration of suspended solids from the backwash water of the microscreen filter could significantly reduce quantity of discharge water. And in some cases, the backwash water from microscreen filters needs to be further concentrated to minimize storage volume during over wintering for land disposal or other final disposal options. In addition, this may be required to meet local, state, and regional discharge water quality. The objective of this research was an initial screening of several commercially available polymers routinely used as coagulation-flocculation aids in the drinking and wastewater treatment industry and determination of their effectiveness for the treatment of aquaculture wastewater. Based on the results of the initial screening, a further evaluation of six polymers was conducted to estimate the optimum polymer dosage for flocculation of aquaculture microscreen effluent and overall solids removal efficiency. Results of these evaluations show TSS removal was close to 99% via settling, with final TSS values ranging from as low as 10-17 mg/L. Although not intended to be used for reactive phosphorus (RP) removal, RP was reduced by 92-95% by removing most of the TSS in the wastewater to approximately 1 mg/L-P. Dosage requirements were fairly uniform, requiring between 15 and 20 mg/L of polymer. Using these dosages, estimated costs range from $4.38 to $13.08 per metric tonne of feed. ?? 2005 Elsevier B.V. All rights reserved.

Description logic-based methods for auditing frame-based medical terminological systems.

PubMed

Cornet, Ronald; Abu-Hanna, Ameen

2005-07-01

Medical terminological systems (TSs) play an increasingly important role in health care by supporting recording, retrieval and analysis of patient information. As the size and complexity of TSs are growing, the need arises for means to audit them, i.e. verify and maintain (logical) consistency and (semantic) correctness of their contents. This is not only important for the management of TSs but also for providing their users with confidence about the reliability of their contents. Formal methods have the potential to play an important role in the audit of TSs, although there are few empirical studies to assess the benefits of using these methods. In this paper we propose a method based on description logics (DLs) for the audit of TSs. This method is based on the migration of the medical TS from a frame-based representation to a DL-based one. Our method is characterized by a process in which initially stringent assumptions are made about concept definitions. The assumptions allow the detection of concepts and relations that might comprise a source of logical inconsistency. If the assumptions hold then definitions are to be altered to eliminate the inconsistency, otherwise the assumptions are revised. In order to demonstrate the utility of the approach in a real-world case study we audit a TS in the intensive care domain and discuss decisions pertaining to building DL-based representations. This case study demonstrates that certain types of inconsistencies can indeed be detected by applying the method to a medical terminological system. The added value of the method described in this paper is that it provides a means to evaluate the compliance to a number of common modeling principles in a formal manner. The proposed method reveals potential modeling inconsistencies, helping to audit and (if possible) improve the medical TS. In this way, it contributes to providing confidence in the contents of the terminological system.

40 CFR 469.27 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... days Milligrams per liter(mg/l) TTO 1 1.37 (2) Arsenic(T) 3 2.09 0.83 Fluoride(T) 32.0 17.4 TSS 61.0 23.0 pH (4) (4) 1 Total toxic organics. 2 Not applicable. 3 The arsenic(T) limitation only applies to...

40 CFR 428.33 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS RUBBER MANUFACTURING POINT SOURCE CATEGORY Solution Crumb...) English units (lb/1,000 lb of product) COD 3.12 2.08 BOD5 0.12 .08 TSS 0.24 .16 Oil and grease 0.12 .08 pH...

Atypical retardation patterns in scanning laser polarimetry are associated with low peripapillary choroidal thickness.

PubMed

Tornow, Ralf P; Schrems, Wolfgang A; Bendschneider, Delia; Horn, Folkert K; Mayer, Markus; Mardin, Christian Y; Lämmer, Robert

2011-09-29

Scanning laser polarimetry (SLP) results can be affected by an atypical retardation pattern (ARP). One reason for an ARP is the birefringence of the sclera. The purpose of this study was to investigate the influence of the peripapillary choroidal thickness (pChTh) on the occurrence of ARP. One hundred ten healthy subjects were investigated with SLP and spectral domain OCT. pChTh was measured in B-scan images at 768 positions using semiautomatic software. Values were averaged to 32 sectors and the total peripapillary mean. Subjects were divided into four groups according to the typical scan score (TSS) provided by the GDxVCC: group 1 TSS, 100; group 2 TSS, 90-99; group 3 TSS, 80-89; group 4 TSS, <80. Mean pChTh (± SD) in 110 healthy subjects was 141 μm (±49 μm). There was a significant correlation between pChTh and TSS (r = 0.608; P < 0.001). In TSS groups 1 to 4, mean pChTh was 168 μm (±38 μm), 148 μm (± 48 μm), 119 μm (±35 μm), and 92 (±42 μm). Mean pChTh of TSS groups 3 and 4 was significantly lower than that of TSS group 1 (P < 0.001). Low values of TSS resulting from the appearance of ARP in SLP are associated with low peripapillary choroidal thickness. Reduced choroidal thickness may result in an increased amount of confounding light getting to the SLP light detectors.

Burkholderia mallei tssM Encodes a Putative Deubiquitinase That Is Secreted and Expressed inside Infected RAW 264.7 Murine Macrophages▿ †

PubMed Central

Shanks, John; Burtnick, Mary N.; Brett, Paul J.; Waag, David M.; Spurgers, Kevin B.; Ribot, Wilson J.; Schell, Mark A.; Panchal, Rekha G.; Gherardini, Frank C.; Wilkinson, Keith D.; DeShazer, David

2009-01-01

Burkholderia mallei, a category B biothreat agent, is a facultative intracellular pathogen that causes the zoonotic disease glanders. The B. mallei VirAG two-component regulatory system activates the transcription of ∼60 genes, including a large virulence gene cluster encoding a type VI secretion system (T6SS). The B. mallei tssM gene encodes a putative ubiquitin-specific protease that is physically linked to, and transcriptionally coregulated with, the T6SS gene cluster. Mass spectrometry and immunoblot analysis demonstrated that TssM was secreted in a virAG-dependent manner in vitro. Surprisingly, the T6SS was found to be dispensable for the secretion of TssM. The C-terminal half of TssM, which contains Cys and His box motifs conserved in eukaryotic deubiquitinases, was purified and biochemically characterized. Recombinant TssM hydrolyzed multiple ubiquitinated substrates and the cysteine at position 102 was critical for enzymatic activity. The tssM gene was expressed within 1 h after uptake of B. mallei into RAW 264.7 murine macrophages, suggesting that the TssM deubiquitinase is produced in this intracellular niche. Although the physiological substrate(s) is currently unknown, the TssM deubiquitinase may provide B. mallei a selective advantage in the intracellular environment during infection. PMID:19168747

Infectious Shock and Toxic Shock Syndrome Diagnoses in Hospitals, Colorado, USA

PubMed Central

Smit, Michael A.; Nyquist, Ann-Christine

2013-01-01

In Colorado, USA, diagnoses coded as toxic shock syndrome (TSS) constituted 27.3% of infectious shock cases during 1993–2006. The incidence of staphylococcal TSS did not change significantly overall or in female patients 10–49 years of age but increased for streptococcal TSS. TSS may be underrecognized among all ages and both sexes. PMID:24188357

Space Shuttle Projects

NASA Image and Video Library

1996-02-23

An STS-75 onboard photo of the Tethered Satellite System-1 Reflight (TSS-1R) atop its extended boom. The TSS-1R was a reflight of TSS-1, which was flown on the Space Shuttle in July/August, 1992. Building on the knowledge gained on the TSS-1 about tether dynamics, the TSS will circle the Earth at an altitude of 296 kilometers (184 miles), placing the tether system well within the rarefield, electrically charged layer of the atmosphere known as the ionosphere. The satellite was plarned to be deployed 20.7 kilometers (12.9 miles) above the Shuttle. The conducting tether, generating high voltage and electrical currents as it moves through the ionosphere cutting magnetic field lines, would allow scientists to examine the electrodynamics of a conducting tether system. In addition, the TSS would increase our understanding of physical processes in the near-Earth space environment, such as plasma waves and currents. The tether on the TSS broke as the Satellite was nearing the full extent of its 12.5 mile deployment from the Shuttle. The TSS was a cooperative development effort by the Italian Space Agency (ASI) and NASA, and was managed by scientists at the Marshall Space Flight Center.

Assessing the ecological effects of human impacts on coral reefs in Bocas del Toro, Panama.

PubMed

Seemann, Janina; González, Cindy T; Carballo-Bolaños, Rodrigo; Berry, Kathryn; Heiss, Georg A; Struck, Ulrich; Leinfelder, Reinhold R

2014-03-01

Environmental and biological reef monitoring was conducted in Almirante Bay (Bahía Almirante) in Bocas del Toro, Panama, to assess impacts from anthropogenic developments. An integrated monitoring investigated how seasonal temperature stress, turbidity, eutrophication and physical impacts threatened reef health and biodiversity throughout the region. Environmental parameters such as total suspended solids [TSS], carbon isotopes (δ(13)C), C/N ratios, chlorophyll a, irradiance, secchi depth, size fractions of the sediments and isotope composition of dissolved inorganic carbon [DIC] of the water were measured throughout the years 2010 and 2011 and were analysed in order to identify different impact sources. Compared to data from Collin et al. (Smithsonian Contributions to the Marine Sciences 38:324-334, 2009) chlorophyll a has doubled at sites close to the city and the port Almirante (from 0.46-0.49 to 0.78-0.97 μg l(-1)) and suspension load increased, visible by a decrease in secchi depth values. Visibility decreased from 9-13 m down to 4 m at the bay inlet Boca del Drago, which is strongly exposed to river run off and dredging for the shipping traffic. Eutrophication and turbidity levels seemed to be the determining factor for the loss of hard coral diversity, most significant at chlorophyll a levels higher than 0.5 μg l(-1) and TSS levels higher than 4.7 mg l(-1). Hard coral cover within the bay has also declined, at some sites down to <10 % with extremely low diversities (7 hard coral species). The hard coral species Porites furcata dominated the reefs in highly impacted areas and showed a strong recovery after bleaching and a higher tolerance to turbidity and eutrophication compared to other hard coral species in the bay. Serious overfishing was detected in the region by a lack of adult and carnivorous fish species, such as grunts, snappers and groupers. Study sites less impacted by anthropogenic activities and/or those with local protection showed a higher hard coral cover and fish abundance; however, an overall loss of hard coral diversity was observed.

Toxic-shock syndrome not associated with menstruation. A review of 54 cases.

PubMed

Reingold, A L; Dan, B B; Shands, K N; Broome, C V

1982-01-02

An increasing proportion of reported cases of toxic-shock syndrome (TSS) are not associated with menstruation (13.2% of reported cases with onset in 1981). The 54 cases of TSS not associated with menstruation reported through the U.S.A. national surveillance system between January, 1980, and June, 1981, were either associated with Staphylococcus aureus infections (cutaneous or subcutaneous lesions, infected surgical wounds, bursitis, mastitis, adenitis, lung abscess, or primary bacteraemia) or followed childbirth by vaginal delivery and caesarean section. Patients with TSS not associated with menstruation differed significantly in age and racial distributions from those with menstruation-associated TSS, and 17 of them were male. The clinical features of TSS not associated with menstruation and the characteristics of the S. aureus strains isolated from these patients were similar to those observed in TSS related to menstruation. The median incubation period of the disease in the post-surgical cases was 2 days. TSS can occur in many clinical settings in patients of both sexes and of all ages and racial groups.

The Boston Harbor Project, and large decreases in loadings of eutrophication-related materials to Boston Harbor.

PubMed

Taylor, David I

2010-04-01

Boston Harbor, a bay-estuary in the north-east USA, has recently been the site of one of the largest wastewater infrastructure projects conducted in the USA, the Boston Harbor Project (BHP). The BHP, which was conducted from 1991 to 2000, ended over a century of direct wastewater treatment facility discharges to the harbor. The BHP caused the loadings of total nitrogen (TN), total phosphorus (TP), total suspended solids (TSS) and particulate organic carbon (POC) to the harbor, to decrease by between 80% and 90%. Approximately one-third of the decreases in TSS and POC loadings occurred between 1991 and 1992; the remaining two-thirds, between 1995 and 2000. For TN and TP, the bulk of the decreases occurred between 1997 or 1998, and 2000. (c) 2009 Elsevier Ltd. All rights reserved.

Tumour suppressor protein p53 regulates the stress activated bilirubin oxidase cytochrome P450 2A6

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hu, Hao, E-mail: hao.hu1@uqconnect.edu.au; Yu, Ting, E-mail: t.yu2@uq.edu.au; Arpiainen, Satu, E-mail: Satu.Juhila@orion.fi

2015-11-15

Human cytochrome P450 (CYP) 2A6 enzyme has been proposed to play a role in cellular defence against chemical-induced oxidative stress. The encoding gene is regulated by various stress activated transcription factors. This paper demonstrates that p53 is a novel transcriptional regulator of the gene. Sequence analysis of the CYP2A6 promoter revealed six putative p53 binding sites in a 3 kb proximate promoter region. The site closest to transcription start site (TSS) is highly homologous with the p53 consensus sequence. Transfection with various stepwise deletions of CYP2A6-5′-Luc constructs – down to − 160 bp from the TSS – showed p53 responsivenessmore » in p53 overexpressed C3A cells. However, a further deletion from − 160 to − 74 bp, including the putative p53 binding site, totally abolished the p53 responsiveness. Electrophoretic mobility shift assay with a probe containing the putative binding site showed specific binding of p53. A point mutation at the binding site abolished both the binding and responsiveness of the recombinant gene to p53. Up-regulation of the endogenous p53 with benzo[α]pyrene – a well-known p53 activator – increased the expression of the p53 responsive positive control and the CYP2A6-5′-Luc construct containing the intact p53 binding site but not the mutated CYP2A6-5′-Luc construct. Finally, inducibility of the native CYP2A6 gene by benzo[α]pyrene was demonstrated by dose-dependent increases in CYP2A6 mRNA and protein levels along with increased p53 levels in the nucleus. Collectively, the results indicate that p53 protein is a regulator of the CYP2A6 gene in C3A cells and further support the putative cytoprotective role of CYP2A6. - Highlights: • CYP2A6 is an immediate target gene of p53. • Six putative p53REs located on 3 kb proximate CYP2A6 promoter region. • The region − 160 bp from TSS is highly homologous with the p53 consensus sequence. • P53 specifically bind to the p53RE on the − 160 bp region. • HNF4α may interact with p53 in regulating CYP2A6 expression.« less

Build-up and wash-off dynamics of atmospherically derived Cu, Pb, Zn and TSS in stormwater runoff as a function of meteorological characteristics.

PubMed

Murphy, Louise U; Cochrane, Thomas A; O'Sullivan, Aisling

2015-03-01

Atmospheric pollutants deposited on impermeable surfaces can be an important source of pollutants to stormwater runoff; however, modelling atmospheric pollutant loads in runoff has rarely been done, because of the challenges and uncertainties in monitoring their contribution. To overcome this, impermeable concrete boards (≈ 1m(2)) were deployed for 11 months in different locations within an urban area (industrial, residential and airside) throughout Christchurch, New Zealand, to capture spatially distributed atmospheric deposition loads in runoff over varying meteorological conditions. Runoff was analysed for total and dissolved Cu, Zn, Pb, and total suspended solids (TSS). Mixed-effect regression models were developed to simulate atmospheric pollutant loads in stormwater runoff. In addition, the models were used to explain the influence of different meteorological characteristics (e.g. antecedent dry days and rain depth) on pollutant build-up and wash-off dynamics. The models predicted approximately 53% to 69% of the variation in pollutant loads and were successful in predicting pollutant-load trends over time which can be useful for general stormwater planning processes. Results from the models illustrated the importance of antecedent dry days on pollutant build-up. Furthermore, results indicated that peak rainfall intensity and rain duration had a significant relationship with TSS and total Pb, whereas, rain depth had a significant relationship with total Cu and total Zn. This suggested that the pollutant speciation phase plays an important role in surface wash-off. Rain intensity and duration had a greater influence when the pollutants were predominantly in their particulate phase. Conversely, rain depth exerted a greater influence when a high fraction of the pollutants were predominantly in their dissolved phase. For all pollutants, the models were represented by a log-arctan relationship for pollutant build-up and a log-log relationship for pollutant wash-off. The modelling approach enables the site-specific relationships between individual pollutants and rainfall characteristics to be investigated. Copyright © 2014 Elsevier B.V. All rights reserved.

Spatial and temporal variation in suspended sediment, organic matter, and turbidity in a Minnesota prairie river: implications for TMDLs.

PubMed

Lenhart, Christian F; Brooks, Kenneth N; Heneley, Daniel; Magner, Joseph A

2010-06-01

The Minnesota River Basin (MRB), situated in the prairie pothole region of the Upper Midwest, contributes excessive sediment and nutrient loads to the Upper Mississippi River. Over 330 stream channels in the MRB are listed as impaired by the Minnesota Pollution Control Agency, with turbidity levels exceeding water quality standards in much of the basin. Addressing turbidity impairment requires an understanding of pollutant sources that drive turbidity, which was the focus of this study. Suspended volatile solids (SVS), total suspended solids (TSS), and turbidity were measured over two sampling seasons at ten monitoring stations in Elm Creek, a turbidity impaired tributary in the MRB. Turbidity levels exceeded the Minnesota standard of 25 nephelometric units in 73% of Elm Creek samples. Turbidity and TSS were correlated (r (2) = 0.76), yet they varied with discharge and season. High levels of turbidity occurred during periods of high stream flow (May-June) because of excessive suspended inorganic sediment from watershed runoff, stream bank, and channel contributions. Both turbidity and TSS increased exponentially downstream with increasing stream power, bank height, and bluff erosion. However, organic matter discharged from wetlands and eutrophic lakes elevated SVS levels and stream turbidity in late summer when flows were low. SVS concentrations reached maxima at lake outlets (50 mg/l) in August. Relying on turbidity measurements alone fails to identify the cause of water quality impairment whether from suspended inorganic sediment or organic matter. Therefore, developing mitigation measures requires monitoring of both TSS and SVS from upstream to downstream reaches.

Quantification of Lycopene, β-Carotene, and Total Soluble Solids in Intact Red-Flesh Watermelon (Citrullus lanatus) Using On-Line Near-Infrared Spectroscopy.

PubMed

Tamburini, Elena; Costa, Stefania; Rugiero, Irene; Pedrini, Paola; Marchetti, Maria Gabriella

2017-04-11

A great interest has recently been focused on lycopene and β-carotene, because of their antioxidant action in the organism. Red-flesh watermelon is one of the main sources of lycopene as the most abundant carotenoid. The use of near-infrared spectroscopy (NIRS) in post-harvesting has permitted us to rapidly quantify lycopene, β-carotene, and total soluble solids (TSS) on single intact fruits. Watermelons, harvested in 2013-2015, were submitted to near-infrared (NIR) radiation while being transported along a conveyor belt system, stationary and in movement, and at different positions on the belt. Eight hundred spectra from 100 samples were collected as calibration set in the 900-1700 nm interval. Calibration models were performed using partial least squares (PLS) regression on pre-treated spectra (derivatives and SNV) in the ranges 2.65-151.75 mg/kg (lycopene), 0.19-9.39 mg/kg (β-carotene), and 5.3%-13.7% (TSS). External validation was carried out with 35 new samples and on 35 spectra. The PLS models for intact watermelon could predict lycopene with R² = 0.877 and SECV = 15.68 mg/kg, β-carotene with R² = 0.822 and SECV = 0.81 mg/kg, and TSS with R² = 0.836 and SECV = 0.8%. External validation has confirmed predictive ability with R² = 0.805 and RMSEP = 16.19 mg/kg for lycopene, R2 = 0.737 and RMSEP = 0.96 mg/kg for β-carotene, and R² = 0.707 and RMSEP = 1.4% for TSS. The results allow for the market valorization of fruits.

Pollutant concentrations and pollution loads in stormwater runoff from different land uses in Chongqing.

PubMed

Wang, Shumin; He, Qiang; Ai, Hainan; Wang, Zhentao; Zhang, Qianqian

2013-03-01

To investigate the distribution of pollutant concentrations and pollution loads in stormwater runoff in Chongqing, six typical land use types were selected and studied from August 2009 to September 2011. Statistical analysis on the distribution of pollutant concentrations in all water samples shows that pollutant concentrations fluctuate greatly in rainfall-runoff, and the concentrations of the same pollutant also vary greatly in different rainfall events. In addition, it indicates that the event mean concentrations (EMCs) of total suspended solids (TSS) and chemical oxygen demand (COD) from urban traffic roads (UTR) are significantly higher than those from residential roads (RR), commercial areas (CA), concrete roofs (CR), tile roofs (TRoof), and campus catchment areas (CCA); and the EMCs of total phosphorus (TP) and NH3-N from UTR and CA are 2.35-5 and 3 times of the class-II standard values specified in the Environmental Quality Standards for Surface Water (GB 3838-2002). The EMCs of Fe, Pb and Cd are also much higher than the class-III standard values. The analysis of pollution load producing coefficients (PLPC) reveals that the main pollution source of TSS, COD and TP is UTR. The analysis of correlations between rainfall factors and EMCs/PLPC indicates that rainfall duration is correlated with EMCs/PLPC of TSS for TRoof and TP for UTR, while rainfall intensity is correlated with EMCs/PLPC of TP for both CR and CCA. The results of this study provide a reference for better management of non-point source pollution in urban regions.

Mesenchymal stromal (stem) cells suppress pro-inflammatory cytokine production but fail to improve survival in experimental staphylococcal toxic shock syndrome.

PubMed

Kim, Hani; Darwish, Ilyse; Monroy, Maria-Fernanda; Prockop, Darwin J; Liles, W Conrad; Kain, Kevin C

2014-01-14

Toxic shock syndrome (TSS) is caused by an overwhelming host-mediated response to bacterial superantigens produced mainly by Staphylococcus aureus and Streptococcus pyogenes. TSS is characterized by aberrant activation of T cells and excessive release of pro-inflammatory cytokines ultimately resulting in capillary leak, septic shock, multiple organ dysfunction and high mortality rates. No therapeutic or vaccine has been approved by the U.S. Food and Drug Administration for TSS, and novel therapeutic strategies to improve clinical outcome are needed. Mesenchymal stromal (stem) cells (MSCs) are stromal cells capable of self-renewal and differentiation. Moreover, MSCs have immunomodulatory properties, including profound effects on activities of T cells and macrophages in specific contexts. Based on the critical role of host-derived immune mediators in TSS, we hypothesized that MSCs could modulate the host-derived proinflammatory response triggered by Staphylococcal enterotoxin B (SEB) and improve survival in experimental TSS. Effects of MSCs on proinflammatory cytokines in peripheral blood were measured in wild-type C57BL/6 mice injected with 50 μg of SEB. Effects of MSCs on survival were monitored in fatal experimental TSS induced by consecutive doses of D-galactosamine (10 mg) and SEB (10 μg) in HLA-DR4 transgenic mice. Despite significantly decreasing serum levels of IL-2, IL-6 and TNF induced by SEB in wild-type mice, human MSCs failed to improve survival in experimental TSS in HLA-DR4 transgenic mice. Similarly, a previously described downstream mediator of human MSCs, TNF-stimulated gene 6 (TSG-6), did not significantly improve survival in experimental TSS. Furthermore, murine MSCs, whether unstimulated or pre-treated with IFNγ, failed to improve survival in experimental TSS. Our results suggest that the immunomodulatory effects of MSCs are insufficient to rescue mice from experimental TSS, and that mediators other than IL-2, IL-6 and TNF are likely to play critical mechanistic roles in the pathogenesis of experimental TSS.

Impact of the spatial resolution of satellite remote sensing sensors in the quantification of total suspended sediment concentration: A case study in turbid waters of Northern Western Australia.

PubMed

Dorji, Passang; Fearns, Peter

2017-01-01

The impact of anthropogenic activities on coastal waters is a cause of concern because such activities add to the total suspended sediment (TSS) budget of the coastal waters, which have negative impacts on the coastal ecosystem. Satellite remote sensing provides a powerful tool in monitoring TSS concentration at high spatiotemporal resolution, but coastal managers should be mindful that the satellite-derived TSS concentrations are dependent on the satellite sensor's radiometric properties, atmospheric correction approaches, the spatial resolution and the limitations of specific TSS algorithms. In this study, we investigated the impact of different spatial resolutions of satellite sensor on the quantification of TSS concentration in coastal waters of northern Western Australia. We quantified the TSS product derived from MODerate resolution Imaging Spectroradiometer (MODIS)-Aqua, Landsat-8 Operational Land Image (OLI), and WorldView-2 (WV2) at native spatial resolutions of 250 m, 30 m and 2 m respectively and coarser spatial resolution (resampled up to 5 km) to quantify the impact of spatial resolution on the derived TSS product in different turbidity conditions. The results from the study show that in the waters of high turbidity and high spatial variability, the high spatial resolution WV2 sensor reported TSS concentration as high as 160 mg L-1 while the low spatial resolution MODIS-Aqua reported a maximum TSS concentration of 23.6 mg L-1. Degrading the spatial resolution of each satellite sensor for highly spatially variable turbid waters led to variability in the TSS concentrations of 114.46%, 304.68% and 38.2% for WV2, Landsat-8 OLI and MODIS-Aqua respectively. The implications of this work are particularly relevant in the situation of compliance monitoring where operations may be required to restrict TSS concentrations to a pre-defined limit.

Impact of the spatial resolution of satellite remote sensing sensors in the quantification of total suspended sediment concentration: A case study in turbid waters of Northern Western Australia

PubMed Central

Fearns, Peter

2017-01-01

The impact of anthropogenic activities on coastal waters is a cause of concern because such activities add to the total suspended sediment (TSS) budget of the coastal waters, which have negative impacts on the coastal ecosystem. Satellite remote sensing provides a powerful tool in monitoring TSS concentration at high spatiotemporal resolution, but coastal managers should be mindful that the satellite-derived TSS concentrations are dependent on the satellite sensor’s radiometric properties, atmospheric correction approaches, the spatial resolution and the limitations of specific TSS algorithms. In this study, we investigated the impact of different spatial resolutions of satellite sensor on the quantification of TSS concentration in coastal waters of northern Western Australia. We quantified the TSS product derived from MODerate resolution Imaging Spectroradiometer (MODIS)-Aqua, Landsat-8 Operational Land Image (OLI), and WorldView-2 (WV2) at native spatial resolutions of 250 m, 30 m and 2 m respectively and coarser spatial resolution (resampled up to 5 km) to quantify the impact of spatial resolution on the derived TSS product in different turbidity conditions. The results from the study show that in the waters of high turbidity and high spatial variability, the high spatial resolution WV2 sensor reported TSS concentration as high as 160 mg L-1 while the low spatial resolution MODIS-Aqua reported a maximum TSS concentration of 23.6 mg L-1. Degrading the spatial resolution of each satellite sensor for highly spatially variable turbid waters led to variability in the TSS concentrations of 114.46%, 304.68% and 38.2% for WV2, Landsat-8 OLI and MODIS-Aqua respectively. The implications of this work are particularly relevant in the situation of compliance monitoring where operations may be required to restrict TSS concentrations to a pre-defined limit. PMID:28380059

Atypical birefringence pattern and the diagnostic ability of scanning laser polarimetry with enhanced corneal compensation in glaucoma.

PubMed

Rao, Harsha L; Yadav, Ravi K; Begum, Viquar U; Addepalli, Uday K; Senthil, Sirisha; Choudhari, Nikhil S; Garudadri, Chandra S

2015-03-01

To evaluate the effect of typical scan score (TSS), when within the acceptable limits, on the diagnostic performance of retinal nerve fibre layer (RNFL) parameters with the enhanced corneal compensation (ECC) protocol of scanning laser polarimetry (SLP) in glaucoma. In a cross-sectional study, 203 eyes of 160 glaucoma patients and 140 eyes of 104 control subjects underwent RNFL imaging with the ECC protocol of SLP. TSS was used to quantify atypical birefringence pattern (ABP) images. Influence of TSS on the diagnostic ability of SLP parameters was evaluated by receiver operating characteristic (ROC) regression models after adjusting for the effect of disease severity [based on mean deviation (MD)] on standard automated perimetry). Diagnostic abilities of all RNFL parameters of SLP increased when the TSS values were higher. This effect was statistically significant for TSNIT (coefficient: 0.08, p<0.001) and inferior average parameters (coefficient: 0.06, p=0.002) but not for nerve fibre indicator (NFI, coefficient: 0.03, p=0.21). In early glaucoma (MD of -5 dB), predicted area under ROC curve (AUC) for TSNIT average parameter improved from 0.642 at a TSS of 90 to 0.845 at a TSS of 100. In advanced glaucoma (MD of -15 dB), AUC for TSNIT average improved from 0.832 at a TSS of 90 to 0.947 at 100. Diagnostic performances of TSNIT and inferior average RNFL parameters with ECC protocol of SLP were significantly influenced by TSS even when the TSS values were within the acceptable limits. Diagnostic ability of NFI was unaffected by TSS values. © 2014 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

Regulation of K+ Transport in Tomato Roots by the TSS1 Locus. Implications in Salt Tolerance1

PubMed Central

Rubio, Lourdes; Rosado, Abel; Linares-Rueda, Adolfo; Borsani, Omar; García-Sánchez, María J.; Valpuesta, Victoriano; Fernández, José A.; Botella, Miguel A.

2004-01-01

The tss1 tomato (Lycopersicon esculentum) mutant exhibited reduced growth in low K+ and hypersensitivity to Na+ and Li+. Increased Ca2+ in the culture medium suppressed the Na+ hypersensitivity and the growth defect on low K+ medium of tss1 seedlings. Interestingly, removing NH4+ from the growth medium suppressed all growth defects of tss1, suggesting a defective NH4+-insensitive component of K+ transport. We performed electrophysiological studies to understand the contribution of the NH4+-sensitive and -insensitive components of K+ transport in wild-type and tss1 roots. Although at 1 mm Ca2+ we found no differences in affinity for K+ uptake between wild type and tss1 in the absence of NH4+, the maximum depolarization value was about one-half in tss1, suggesting that a set of K+ transporters is inactive in the mutant. However, these transporters became active by raising the external Ca2+ concentration. In the presence of NH4+, a reduced affinity for K+ was observed in both types of seedlings, but tss1 at 1 mm Ca2+ exhibited a 2-fold higher Km than wild type did. This defect was again corrected by raising the external concentration of Ca2+. Therefore, membrane potential measurements in root cells indicated that tss1 is affected in both NH4+-sensitive and -insensitive components of K+ transport at low Ca2+ concentrations and that this defective transport is rescued by increasing the concentration of Ca2+. Our results suggest that the TSS1 gene product is part of a crucial pathway mediating the beneficial effects of Ca2+ involved in K+ nutrition and salt tolerance. PMID:14684839

Theory and Modeling in Support of Tether

NASA Technical Reports Server (NTRS)

Chang, C. L.; Bergeron, G.; Drobot, A. D.; Papadopoulos, K.; Riyopoulos, S.; Szuszczewicz, E.

1999-01-01

This final report summarizes the work performed by SAIC's Applied Physics Operation on the modeling and support of Tethered Satellite System missions (TSS-1 and TSS-1R). The SAIC team, known to be Theory and Modeling in Support of Tether (TMST) investigation, was one of the original twelve teams selected in July, 1985 for the first TSS mission. The accomplishments described in this report cover the period December 19, 1985 to September 31, 1999 and are the result of a continuous effort aimed at supporting the TSS missions in the following major areas. During the contract period, the SAIC's TMST investigation acted to: Participate in the planning and the execution on both of the TSS missions; Provide scientific understanding on the issues involved in the electrodynamic tether system operation prior to the TSS missions; Predict ionospheric conditions encountered during the re-flight mission (TSS-lR) based on realtime global ionosounde data; Perform post mission analyses to enhance our understanding on the TSS results. Specifically, we have 1) constructed and improved current collection models and enhanced our understanding on the current-voltage data; 2) investigated the effects of neutral gas in the current collection processes; 3) conducted laboratory experiments to study the discharge phenomena during and after tether-break; and 4) perform numerical simulations to understand data collected by plasma instruments SPES onboard the TSS satellite; Design and produce multi-media CD that highlights TSS mission achievements and convey the knowledge of the tether technology to the general public. Along with discussions of this work, a list of publications and presentations derived from the TMST investigation spanning the reporting period is compiled.

Theoretical Analysis of Competing Conformational Transitions in Superhelical DNA

PubMed Central

Zhabinskaya, Dina; Benham, Craig J.

2012-01-01

We develop a statistical mechanical model to analyze the competitive behavior of transitions to multiple alternate conformations in a negatively supercoiled DNA molecule of kilobase length and specified base sequence. Since DNA superhelicity topologically couples together the transition behaviors of all base pairs, a unified model is required to analyze all the transitions to which the DNA sequence is susceptible. Here we present a first model of this type. Our numerical approach generalizes the strategy of previously developed algorithms, which studied superhelical transitions to a single alternate conformation. We apply our multi-state model to study the competition between strand separation and B-Z transitions in superhelical DNA. We show this competition to be highly sensitive to temperature and to the imposed level of supercoiling. Comparison of our results with experimental data shows that, when the energetics appropriate to the experimental conditions are used, the competition between these two transitions is accurately captured by our algorithm. We analyze the superhelical competition between B-Z transitions and denaturation around the c-myc oncogene, where both transitions are known to occur when this gene is transcribing. We apply our model to explore the correlation between stress-induced transitions and transcriptional activity in various organisms. In higher eukaryotes we find a strong enhancement of Z-forming regions immediately 5′ to their transcription start sites (TSS), and a depletion of strand separating sites in a broad region around the TSS. The opposite patterns occur around transcript end locations. We also show that susceptibility to each type of transition is different in eukaryotes and prokaryotes. By analyzing a set of untranscribed pseudogenes we show that the Z-susceptibility just downstream of the TSS is not preserved, suggesting it may be under selection pressure. PMID:22570598

Toxic shock syndrome in Australian children.

PubMed

Chen, Katherine Y H; Cheung, Michael; Burgner, David P; Curtis, Nigel

2016-08-01

There are limited data describing the epidemiology, management and outcomes of children with toxic shock syndrome (TSS), a potentially life-threatening illness. Here, we describe the incidence, clinical features, treatment and outcome of children with staphylococcal and streptococcal TSS in Victoria, Australia. Retrospective chart review of children admitted between 2003 and 2014 to two tertiary paediatric referral centres who fulfilled the Centers for Disease Control and Prevention case definition of TSS. There were 62 cases over the 11-year period; 43 staphylococcal TSS and 19 streptococcal TSS. The majority (46 (74%)) of cases were admitted to an intensive care unit and 44 (71%) required inotropic support. Compared with those with staphylococcal TSS, patients with streptococcal TSS were younger, more unwell and more likely to have residual morbidity. Adjunctive therapy with intravenous immunoglobulin or clindamycin was used in 58 (94%) of cases and fresh frozen plasma was used in 15 (24%). Seven patients received extracorporeal membrane oxygenation. All patients survived. This study highlights the importance of early recognition of TSS and supports rapid tertiary referral and intensive care management, which includes the use of adjunctive therapy with intravenous immunoglobulin and clindamycin. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

Monitoring and predicting the fecal indicator bacteria concentrations from agricultural, mixed land use and urban stormwater runoff.

PubMed

Paule-Mercado, M A; Ventura, J S; Memon, S A; Jahng, D; Kang, J-H; Lee, C-H

2016-04-15

While the urban runoff are increasingly being studied as a source of fecal indicator bacteria (FIB), less is known about the occurrence of FIB in watershed with mixed land use and ongoing land use and land cover (LULC) change. In this study, Escherichia coli (EC) and fecal streptococcus (FS) were monitored from 2012 to 2013 in agricultural, mixed and urban LULC and analyzed according to the most probable number (MPN). Pearson correlation was used to determine the relationship between FIB and environmental parameters (physicochemical and hydrometeorological). Multiple linear regressions (MLR) were used to identify the significant parameters that affect the FIB concentrations and to predict the response of FIB in LULC change. Overall, the FIB concentrations were higher in urban LULC (EC=3.33-7.39; FS=3.30-7.36log10MPN/100mL) possibly because of runoff from commercial market and 100% impervious cover (IC). Also, during early-summer season; this reflects a greater persistence and growth rate of FIB in a warmer environment. During intra-event, however, the FIB concentrations varied according to site condition. Anthropogenic activities and IC influenced the correlation between the FIB concentrations and environmental parameters. Stormwater temperature (TEMP), turbidity, and TSS positively correlated with the FIB concentrations (p>0.01), since IC increased, implying an accumulation of bacterial sources in urban activities. TEMP, BOD5, turbidity, TSS, and antecedent dry days (ADD) were the most significant explanatory variables for FIB as determined in MLR, possibly because they promoted the FIB growth and survival. The model confirmed the FIB concentrations: EC (R(2)=0.71-0.85; NSE=0.72-0.86) and FS (R(2)=0.65-0.83; NSE=0.66-0.84) are predicted to increase due to urbanization. Therefore, these findings will help in stormwater monitoring strategies, designing the best management practice for FIB removal and as input data for stormwater models. Copyright © 2016 Elsevier B.V. All rights reserved.

Prevalence of toxic shock syndrome toxin 1-producing Staphylococcus aureus and the presence of antibodies to this superantigen in menstruating women.

PubMed

Parsonnet, Jeffrey; Hansmann, Melanie A; Delaney, Mary L; Modern, Paul A; Dubois, Andrea M; Wieland-Alter, Wendy; Wissemann, Kimberly W; Wild, John E; Jones, Michaelle B; Seymour, Jon L; Onderdonk, Andrew B

2005-09-01

Menstrual toxic shock syndrome (mTSS) is thought to be associated with colonization with toxic shock syndrome toxin 1 (TSST-1)-producing Staphylococcus aureus in women with insufficient antibody titers. mTSS has been associated with menstruation and tampon use, and although it is rare, the effects can be life threatening. It remains of interest because of the widespread use of tampons, reported to be about 70% of women in the United States, Canada, and much of Western Europe. This comprehensive study was designed to determine S. aureus colonization and TSST-1 serum antibody titers in 3,012 menstruating women in North America between the ages of 13 and 40, particularly among age and racial groups that could not be assessed reliably in previous small studies. One out of every four subjects was found to be colonized with S. aureus in at least one of three body sites (nose, vagina, or anus), with approximately 9% colonized vaginally. Eighty-five percent of subjects had antibody titers (> or =1:32) to TSST-1, and the vast majority (81%) of teenaged subjects (13 to 18 years) had already developed antibody titers. Among carriers of toxigenic S. aureus, a significantly lower percentage of black women than of white or Hispanic women were found to have antibody titers (> or =1:32) to TSST-1 (89% versus 98% and 100%). These findings demonstrate that the majority of teenagers have antibody titers (> or =1:32) to TSST-1 and are presumed to be protected from mTSS. These findings also suggest that black women may be more susceptible to mTSS than previously thought.

Prevalence of Toxic Shock Syndrome Toxin 1-Producing Staphylococcus aureus and the Presence of Antibodies to This Superantigen in Menstruating Women

PubMed Central

Parsonnet, Jeffrey; Hansmann, Melanie A.; Delaney, Mary L.; Modern, Paul A.; DuBois, Andrea M.; Wieland-Alter, Wendy; Wissemann, Kimberly W.; Wild, John E.; Jones, Michaelle B.; Seymour, Jon L.; Onderdonk, Andrew B.

2005-01-01

Menstrual toxic shock syndrome (mTSS) is thought to be associated with colonization with toxic shock syndrome toxin 1 (TSST-1)-producing Staphylococcus aureus in women with insufficient antibody titers. mTSS has been associated with menstruation and tampon use, and although it is rare, the effects can be life threatening. It remains of interest because of the widespread use of tampons, reported to be about 70% of women in the United States, Canada, and much of Western Europe. This comprehensive study was designed to determine S. aureus colonization and TSST-1 serum antibody titers in 3,012 menstruating women in North America between the ages of 13 and 40, particularly among age and racial groups that could not be assessed reliably in previous small studies. One out of every four subjects was found to be colonized with S. aureus in at least one of three body sites (nose, vagina, or anus), with approximately 9% colonized vaginally. Eighty-five percent of subjects had antibody titers (≥1:32) to TSST-1, and the vast majority (81%) of teenaged subjects (13 to 18 years) had already developed antibody titers. Among carriers of toxigenic S. aureus, a significantly lower percentage of black women than of white or Hispanic women were found to have antibody titers (≥1:32) to TSST-1 (89% versus 98% and 100%). These findings demonstrate that the majority of teenagers have antibody titers (≥1:32) to TSST-1 and are presumed to be protected from mTSS. These findings also suggest that black women may be more susceptible to mTSS than previously thought. PMID:16145118

40 CFR 424.25 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... and Other Smelting Operations With Wet Air Pollution Control Devices Subcategory § 424.25 Standards of... 0.016 Chromium total .001 .0005 Chromium VI .0001 .00005 Manganese total .011 .005 Cyanide total .0005 .0003 Phenols .0004 .0002 pH (1) (1) English units (lb/Mwh) TSS .071 .035 Chromium total .002...

40 CFR 424.25 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... and Other Smelting Operations With Wet Air Pollution Control Devices Subcategory § 424.25 Standards of... 0.016 Chromium total .001 .0005 Chromium VI .0001 .00005 Manganese total .011 .005 Cyanide total .0005 .0003 Phenols .0004 .0002 pH (1) (1) English units (lb/Mwh) TSS .071 .035 Chromium total .002...

40 CFR 424.25 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 0.016 Chromium total .001 .0005 Chromium VI .0001 .00005 Manganese total .011 .005 Cyanide total .0005 .0003 Phenols .0004 .0002 pH (1) (1) English units (lb/Mwh) TSS .071 .035 Chromium total .002 .0012 Chromium VI .0002 .0001 Manganese total .023 .012 Cyanide total .001 .0006 Phenols .0009 .0005 pH...

40 CFR 424.25 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 0.016 Chromium total .001 .0005 Chromium VI .0001 .00005 Manganese total .011 .005 Cyanide total .0005 .0003 Phenols .0004 .0002 pH (1) (1) English units (lb/Mwh) TSS .071 .035 Chromium total .002 .0012 Chromium VI .0002 .0001 Manganese total .023 .012 Cyanide total .001 .0006 Phenols .0009 .0005 pH...

40 CFR 424.25 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 0.016 Chromium total .001 .0005 Chromium VI .0001 .00005 Manganese total .011 .005 Cyanide total .0005 .0003 Phenols .0004 .0002 pH (1) (1) English units (lb/Mwh) TSS .071 .035 Chromium total .002 .0012 Chromium VI .0002 .0001 Manganese total .023 .012 Cyanide total .001 .0006 Phenols .0009 .0005 pH...

Evaluation of accuracy of linear regression models in predicting urban stormwater discharge characteristics.

PubMed

Madarang, Krish J; Kang, Joo-Hyon

2014-06-01

Stormwater runoff has been identified as a source of pollution for the environment, especially for receiving waters. In order to quantify and manage the impacts of stormwater runoff on the environment, predictive models and mathematical models have been developed. Predictive tools such as regression models have been widely used to predict stormwater discharge characteristics. Storm event characteristics, such as antecedent dry days (ADD), have been related to response variables, such as pollutant loads and concentrations. However it has been a controversial issue among many studies to consider ADD as an important variable in predicting stormwater discharge characteristics. In this study, we examined the accuracy of general linear regression models in predicting discharge characteristics of roadway runoff. A total of 17 storm events were monitored in two highway segments, located in Gwangju, Korea. Data from the monitoring were used to calibrate United States Environmental Protection Agency's Storm Water Management Model (SWMM). The calibrated SWMM was simulated for 55 storm events, and the results of total suspended solid (TSS) discharge loads and event mean concentrations (EMC) were extracted. From these data, linear regression models were developed. R(2) and p-values of the regression of ADD for both TSS loads and EMCs were investigated. Results showed that pollutant loads were better predicted than pollutant EMC in the multiple regression models. Regression may not provide the true effect of site-specific characteristics, due to uncertainty in the data. Copyright © 2014 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.

Electric Transport Traction Power Supply System With Distributed Energy Sources

NASA Astrophysics Data System (ADS)

Abramov, E. Y.; Schurov, N. I.; Rozhkova, M. V.

2016-04-01

The paper states the problem of traction substation (TSS) leveling of daily-load curve for urban electric transport. The circuit of traction power supply system (TPSS) with distributed autonomous energy source (AES) based on photovoltaic (PV) and energy storage (ES) units is submitted here. The distribution algorithm of power flow for the daily traction load curve leveling is also introduced in this paper. In addition, it illustrates the implemented experiment model of power supply system.

Investigators unable to substantiate suspected link between sponge, TSS.

PubMed

1983-12-01

Federal investigators have failed to substantiate a suspected link between the contraceptive sponge and toxic shock syndrome (TSS). In September the US Food and Drug Administration (FDA) reported the case of a woman who inserted the contraceptive sponge last July 17 and removed itthe following day. About 6 hours later she noted the sudden onset of a fever of 104 degrees Farenheit, nausea, redness, shaking chills, and an inflamed vagina. Cultures from the sponge revealed S. aureus and S. epidemis. There was initial concern that the case may have represented early TSS. A toxin produced by certain strains of S. aureus is thought to cause TSS. The syndrome includes a fever greater than 102 degrees, rash, blood pressure less than 90mmHG, peelin g skin on the palms and soles 1-2 weeks after onset, and involvement of 3 or more of the following organ systems: gastrointestinal, muscular, mucous membrane, renal, hepatic, hematologic,or central nervous system. FDA medical Officers Dr. William J. McCann told "Contraceptive Technology Update" (CTU) that the reported case failed to fill the Centers for Disease Control criteriaof the diagnosis of TSS. Because the woman has been treated with antibiotics early in the course of her disease, McCann said he could not entirely exclude the possibility that she might have developed TSS if she had gone untreated. He added that the possibility was "remote". Dr. Gail Bolan, CDC epidemiologist, told CTU that "antibiotics do not seem to affect the outcome of the original episode" of TSS cases. She commented that milder forms of TSS might exist that may not meet CDC's strict case definition. Without a specific test, there is no way to separate milder TSS cases from viral or other diseases that may appear similar. According to Deborah Gaynor, the sponge's package insert states that clinical trials were not large enough to assess the risk of TSS. The sponge is not recommended for use during menstration. Gaynor cites a variety of reasons why the contraceptive sponge does not cause TSS: Remarketing trials of the sponge included more than 1000 women years of experience without a reported case of TSS; nonoxynol 9 spermicide and citric, ascorbic, and benzoic acids in the sponge are hostile to S. aureus; unlike tampons, the sponge is not highly absorbant and does not cause microabrasions of the vagina; and during testing, contraceptive sponges were innoculated with the Harrisburg strain of S. aureus known to cause TSS fatality, and after incubation in growth medium for 28 days the S. aureus failed to grow in the innoculated sponges.

TSSAR: TSS annotation regime for dRNA-seq data.

PubMed

Amman, Fabian; Wolfinger, Michael T; Lorenz, Ronny; Hofacker, Ivo L; Stadler, Peter F; Findeiß, Sven

2014-03-27

Differential RNA sequencing (dRNA-seq) is a high-throughput screening technique designed to examine the architecture of bacterial operons in general and the precise position of transcription start sites (TSS) in particular. Hitherto, dRNA-seq data were analyzed by visualizing the sequencing reads mapped to the reference genome and manually annotating reliable positions. This is very labor intensive and, due to the subjectivity, biased. Here, we present TSSAR, a tool for automated de novo TSS annotation from dRNA-seq data that respects the statistics of dRNA-seq libraries. TSSAR uses the premise that the number of sequencing reads starting at a certain genomic position within a transcriptional active region follows a Poisson distribution with a parameter that depends on the local strength of expression. The differences of two dRNA-seq library counts thus follow a Skellam distribution. This provides a statistical basis to identify significantly enriched primary transcripts.We assessed the performance by analyzing a publicly available dRNA-seq data set using TSSAR and two simple approaches that utilize user-defined score cutoffs. We evaluated the power of reproducing the manual TSS annotation. Furthermore, the same data set was used to reproduce 74 experimentally validated TSS in H. pylori from reliable techniques such as RACE or primer extension. Both analyses showed that TSSAR outperforms the static cutoff-dependent approaches. Having an automated and efficient tool for analyzing dRNA-seq data facilitates the use of the dRNA-seq technique and promotes its application to more sophisticated analysis. For instance, monitoring the plasticity and dynamics of the transcriptomal architecture triggered by different stimuli and growth conditions becomes possible.The main asset of a novel tool for dRNA-seq analysis that reaches out to a broad user community is usability. As such, we provide TSSAR both as intuitive RESTful Web service ( http://rna.tbi.univie.ac.at/TSSAR) together with a set of post-processing and analysis tools, as well as a stand-alone version for use in high-throughput dRNA-seq data analysis pipelines.

Septic wastewater treatment using recycled rubber particles as biofiltration media.

PubMed

Oh, Jin Hwan; Park, Jaeyoung; Ellis, Timothy G

2014-01-01

Performance of the laboratory-scale recycled rubber particles (RRP) biofilter was compared to a conventional gravel system and a peat biofilter for treatment of septic tank effluent. During the study, the RRP biofilter provided similar or better performance than other systems in terms of organic removal and hydraulic capacity. After the start-up period, RRP biofilter achieved removal efficiencies for BOD5, total suspended solids (TSS), ammonia nitrogen of 96%, 93%, and 90%, respectively, over the range of hydraulic loading rates of 57-204 L/m2/d. On the other hand, the peat biofilter failed hydraulically and the gravel system showed high TSS concentrations in the effluent. RRP provided high surface area and sufficient time for biological treatment. In addition, RRP was observed to provide ammonia adsorption capacity. The results showed that RRP has the potential to be used as substitutes for natural aggregate such as gravel in septic system drainfields. The RRP biofilter can be used as alternative septic systems for the sites where an existing septic system has failed or site conditions, such as high groundwater table or small lot size, are not suitable for the installation of conventional septic systems.

[Research on stormwater runoff quality of mountain city by source area monitoring].

PubMed

Li, Li-Qing; Shan, Bao-Qing; Zhao, Jian-Wei; Guo, Shu-Gang; Gao, Yong

2012-10-01

Stormwater runoff samples were collected from 10 source areas in Mountain City, Chongqing, during five rain events in an attempt to investigate the characteristics of runoff quality and influencing factors. The outcomes are expected to offer practical guidance of sources control of urban runoff pollution. The results indicated that the stormwater runoff of Mountain City presented a strong first flush for almost all events and constituents. The runoff quality indices were also influenced by the rainfall intensity. The concentration of TSS, COD, TN and TP decreased as the rainfall intensity increased. The concentrations of COD and TP in stormwater runoff were highly correlated with TSS concentrations. Suspended solid matter were not only the main pollutant of stormwater runoff but also served as the vehicle for transport of organic matter and phosphorus. Organic matter and phosphorus in stormwatrer runoff were mainly bound to particles, whereas nitrogen was predominantly dissolved, with ammonia and nitrate. A significant difference of stormwater runoff quality was observed among the ten monitored source areas. The highest magnitude of urban stormwater runoff pollution was expected in the commercial area and the first trunk road, followed by the minor road, residential area, parking lot and roof. Urban surface function, traffic volume, population density, and street sweeping practice are the main factors determining spatial differentiation of urban surface runoff quality. Commercial area, the first trunk road and residential area with high population density are the critical sources areas of urban stormwater runoff pollution.

Operating microscope light-induced phototoxic maculopathy after transscleral sutured posterior chamber intraocular lens implantation.

PubMed

Kweon, Eui Yong; Ahn, Min; Lee, Dong Wook; You, In Cheon; Kim, Min Jung; Cho, Nam Chun

2009-01-01

The purpose of this study is to report the features of operating microscope light-induced retinal phototoxic maculopathy after transscleral sutured posterior chamber intraocular lens (TSS PC-IOL) implantation. The charts of 118 patients who underwent TSS PC-IOL implantation surgery at Chonbuk National University Hospital (Jeonju, Korea) between March 1999 and February 2008 were retrospectively reviewed. Fourteen patients underwent combined 3-port pars plana vitrectomy and TSS PC-IOL implantation (vitrectomy group), and 104 patients underwent TSS PC-IOL implantation only (nonvitrectomy group). All surgeries were performed under the same coaxial illuminated microscope. All diagnoses were confirmed through careful fundus examination and fluorescein angiography (FA). Diagnoses of retinal phototoxic maculopathy were established in 10 (8.47%) of 118 TSS PC-IOL implantation cases. Phototoxic maculopathy occurred more frequently in the vitrectomy group than in the nonvitrectomy group (6/14 versus 4/104, respectively; P < 0.001, chi-square = 24.21). Affected patients reported decreased vision and were found to have coarse alterations of the retinal pigment epithelium (RPE). In 5 of the phototoxic maculopathy cases (50%), the visual acuity was 20/200 or worse. Operating microscope light-induced retinal phototoxic maculopathy can occur more frequently after TSS PC-IOL implantation than after casual cataract surgery, especially when TSS PC-IOL is combined with vitrectomy surgery. Surgeons should take precautions to prevent retinal phototoxicity after TSS PC-IOL implantation and vitrectomy.

Comparison between snowmelt-runoff and rainfall-runoff nonpoint source pollution in a typical urban catchment in Beijing, China.

PubMed

Chen, Lei; Zhi, Xiaosha; Shen, Zhenyao; Dai, Ying; Aini, Guzhanuer

2018-01-01

As a climate-driven event, nonpoint source (NPS) pollution is caused by rainfall- or snowmelt-runoff processes; however, few studies have compared the characteristics and mechanisms of these two kinds of NPS processes. In this study, three factors relating to urban NPS, including surface dust, snowmelt, and rainfall-runoff processes, were analyzed comprehensively by both field sampling and laboratory experiments. The seasonal variation and leaching characteristics of pollutants in surface dust were explored, and the runoff quality of snowmelt NPS and rainfall NPS were compared. The results indicated that dusts are the main sources of urban NPS and more pollutants are deposited in dust samples during winter and spring. However, pollutants in surface dust showed a low leaching ratio, which indicated most NPS pollutants would be carried as particulate forms. Compared to surface layer, underlying snow contained higher chemical oxygen demand, total suspended solids (TSS), Cu, Fe, Mn, and Pb concentrations, while the event mean concentration of most pollutants in snowmelt tended to be higher in roads. Moreover, the TSS and heavy metal content of snowmelt NPS was always higher than those of rainfall NPS, which indicated the importance of controlling snowmelt pollution for effective water quality management.

Adjustment of total suspended solids data for use in sediment studies

USGS Publications Warehouse

Glysson, G. Douglas; Gray, John R.; Conge, L.M.; Hotchkiss, Rollin H.; Glade, Michael

2000-01-01

The U.S. Environmental Protection Agency identifies fluvial sediment as the single most widespread pollutant in the Nation's rivers and streams, affecting aquatic habitat, drinking water treatment processes, and recreational uses of rivers, lakes, and estuaries. A significant amount of suspended-sediment data has been produced using the total suspended solids (TSS) laboratory analysis method. An evaluation of data collected and analyzed by the U.S. Geological Survey and others has shown that the variation in TSS analytical results is considerably larger than that for traditional suspended-sediment concentration analyses (SSC) and that the TSS data show a negative bias when compared to SSC data. This paper presents the initial results of a continuing investigation into the differences between TSS and SSC results. It explores possible relations between these differences and other hydrologic data collected at the same stations. A general equation was developed to relate TSS data to SSC data. However, this general equation is not applicable for data from individual stations. Based on these analyses, there appears to be no simple, straightforward way to relate TSS and SSC data unless pairs of TSS and SSC results are available for a station.

Identifying Distribution of Chlorophyll-a Concentration Using Landsat 8 OLI on Marine Waters Area of Cirebon

NASA Astrophysics Data System (ADS)

Buditama, Gilang; Damayanti, Astrid; Giok Pin, Tjiong

2017-12-01

Phytoplankton is a microscopic plant that has a function to produces oxygen and organic substances. It also plays a role as a main producer in the food chain of a marine ecosystem. Chlorophyll-a is a colour pigment most common in phytoplankton species, so that the concentration level of chlorophyll-a can be used as an indicator of the abundance of phytoplankton cells, and as a reference for predicting organic potency in the aquatic area. This research discusses about the spatial and temporal distribution of chlorophyll-a and its correlation with salinity and total suspended solid (TSS), in the seawaters of Cirebon, West Java. The goal of this research is to be a source of information for fishermen, and other stakeholders whose related subjects in the field of marine and fisheries to predict fertile water regions and can also be used as an indicator in discovering potential areas to catch pelagic fish in Cirebon seawaters. Chlorophyll-a concentration, salinity, and TSS are identified using remote sensing data such as Landsat-8 OLI multi temporal images according to dry and wet month parameters in the 2014-2015. The results of the processed image are then validated between in-situ measurements in the field and remote sensing imagery at the same time. This research utilizes descriptive analysis, and statistics with spatial approach. The results of the research show that temporally, chlorophyll-a levels have a tendency to be higher in wet months compared to dry months, while chlorophyll-a is higher in areas near the coastline compared to open sea areas. The distribution of chlorophyll-a concentration is affected by salinity and TSS distribution, where salinity has the negative relationship and TSS has the positive relationship with chlorophyll-a distribution.

Temporal variability of combined sewer overflow contaminants: evaluation of wastewater micropollutants as tracers of fecal contamination.

PubMed

Madoux-Humery, Anne-Sophie; Dorner, Sarah; Sauvé, Sébastien; Aboulfadl, Khadija; Galarneau, Martine; Servais, Pierre; Prévost, Michèle

2013-09-01

A monitoring program was initiated for two sewage outfalls (OA and OB) with different land uses (mainly residential versus institutional) over the course of a year. Eleven CSO events resulting from fall and summer precipitations and a mixture of snowmelt and precipitation in late winter and early spring were monitored. Median concentrations measured in CSOs were 1.5 × 10(6)Escherichia coli/100 mL, 136.0 mg/L of Total Suspended Solids (TSS), 4599.0 ng/L of caffeine (CAF), 158.9 ng/L of carbamazepine (CBZ), in outfall OA and 5.1 × 10(4)E. coli/100 mL, 167.0 mg TSS/L, 300.8 ng CAF/L, 4.1 ng CBZ/L, in outfall OB. Concentration dynamics in CSOs were mostly related to the dilution by stormwater and the time of day of the onset of overflows. Snowmelt was identified as a critical period with regards to the protection of drinking water sources given the high contaminant concentrations and long duration of events in addition to a lack of restrictions on overflows during this period. Correlations among measured parameters reflected the origins and transport pathways of the contaminants, with E. coli being correlated with CBZ. TSS were not correlated with E. coli because E. coli was found to be mostly associated with raw sewage whereas TSS were additionally from the resuspension of in-sewer deposits and surface runoff. In receiving waters, E. coli remained the best indicator of fecal contamination in strongly diluted water samples as compared to WWMPs because WWMPs can be diluted to below their detection limits. Copyright © 2013 Elsevier Ltd. All rights reserved.

Efficient MRI labeling of endothelial progenitor cells: design of thiolated surface stabilized superparamagnetic iron oxide nanoparticles.

PubMed

Shahnaz, Gul; Kremser, Christian; Reinisch, Andreas; Vetter, Anja; Laffleur, Flavia; Rahmat, Deni; Iqbal, Javed; Dünnhaupt, Sarah; Salvenmoser, Willi; Tessadri, Richard; Griesser, Ulrich; Bernkop-Schnürch, Andreas

2013-11-01

The aim of this study was to design thiolated surface stabilized superparamagnetic iron oxide nanoparticles (TSS-SPIONs) for efficient internalization with high MRI sensitivity. TSS-SPIONs were developed by chelation between thiolated chitosan-thioglycolic acid (chitosan-TGA) hydrogel and iron ions (Fe(2+)/Fe(3+)). Likely, unmodified chitosan hydrogel SPIONs (UC-SPIONs) and uncoated SPIONs were used as control. Moreover, TSS-SPIONs were investigated regarding to their iron core size, hydrodynamic diameter, zeta potential, iron contents, molar relaxivities (r1 and r2), and cellular internalization. TSS-SPIONs demonstrated an iron oxide core diameter (crystallite size by XRD) of 3.1 ± 0.02 nm, a hydrodynamic diameter of 94 ± 20 nm, a zeta potential of +21 ± 5 mV, and an iron content of 3.6 ± 0.9 mg/mL. In addition, internalization of TSS-SPIONs into human endothelial progenitor cells (EPC) from umbilical cord blood was more than threefold and 17-fold higher in contrast to UC-SPIONs and SPIONs, respectively. With twofold lower incubation iron concentration of TSS-SPIONs, more than threefold higher internalization was achieved as compared to Resovist®. Also, cell viability of more than 90% was observed in the presence of TSS-SPIONs after 24h. The molar MR relaxivities (r2) value at 1.5 T was threefold higher than that of Resovist® and demonstrated that TSS-SPIONs have the potential as very effective T2 contrast-enhancement agent. According to these findings, TSS-SPIONs with efficient internalization, lower cytotoxicity, and high MRI sensitivity seem to be promising for cell tracking. Copyright © 2013 Elsevier B.V. All rights reserved.

Developing the remote sensing-based early warning system for monitoring TSS concentrations in Lake Mead.

PubMed

Imen, Sanaz; Chang, Ni-Bin; Yang, Y Jeffrey

2015-09-01

Adjustment of the water treatment process to changes in water quality is a focus area for engineers and managers of water treatment plants. The desired and preferred capability depends on timely and quantitative knowledge of water quality monitoring in terms of total suspended solids (TSS) concentrations. This paper presents the development of a suite of nowcasting and forecasting methods by using high-resolution remote-sensing-based monitoring techniques on a daily basis. First, the integrated data fusion and mining (IDFM) technique was applied to develop a near real-time monitoring system for daily nowcasting of the TSS concentrations. Then a nonlinear autoregressive neural network with external input (NARXNET) model was selected and applied for forecasting analysis of the changes in TSS concentrations over time on a rolling basis onward using the IDFM technique. The implementation of such an integrated forecasting and nowcasting approach was assessed by a case study at Lake Mead hosting the water intake for Las Vegas, Nevada, in the water-stressed western U.S. Long-term monthly averaged results showed no simultaneous impact from forest fire events on accelerating the rise of TSS concentration. However, the results showed a probable impact of a decade of drought on increasing TSS concentration in the Colorado River Arm and Overton Arm. Results of the forecasting model highlight the reservoir water level as a significant parameter in predicting TSS in Lake Mead. In addition, the R-squared value of 0.98 and the root mean square error of 0.5 between the observed and predicted TSS values demonstrates the reliability and application potential of this remote sensing-based early warning system in terms of TSS projections at a drinking water intake. Copyright © 2015 Elsevier Ltd. All rights reserved.

40 CFR 420.124 - New source performance standards (NSPS).

Code of Federal Regulations, 2012 CFR

2012-07-01

... Zinc 0.000376 0.000125 Chromium (hexavalent) 1 0.0000376 0.0000125 pH (2) (2) 1 The limitations for hexavalent chromium shall be applicable only to galvanizing operations which discharge wastewaters from the... product TSS 0.175 0.0751 O&G 0.0751 0.0250 Lead 0.00113 0.000376 Zinc 0.00150 0.000500 Chromium...

40 CFR 420.124 - New source performance standards (NSPS).

Code of Federal Regulations, 2013 CFR

2013-07-01

... Zinc 0.000376 0.000125 Chromium (hexavalent) 1 0.0000376 0.0000125 pH (2) (2) 1 The limitations for hexavalent chromium shall be applicable only to galvanizing operations which discharge wastewaters from the... product TSS 0.175 0.0751 O&G 0.0751 0.0250 Lead 0.00113 0.000376 Zinc 0.00150 0.000500 Chromium...

40 CFR 420.124 - New source performance standards (NSPS).

Code of Federal Regulations, 2014 CFR

2014-07-01

... Zinc 0.000376 0.000125 Chromium (hexavalent) 1 0.0000376 0.0000125 pH (2) (2) 1 The limitations for hexavalent chromium shall be applicable only to galvanizing operations which discharge wastewaters from the... product TSS 0.175 0.0751 O&G 0.0751 0.0250 Lead 0.00113 0.000376 Zinc 0.00150 0.000500 Chromium...

40 CFR 419.36 - Standards of performance for new sources (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... grease 6.6 3.5 Phenolic compounds 0.158 .077 Ammonia as N 23.4 10.7 Sulfide 0.140 0.063 Total chromium 0...) BOD5 7.7 4.1 TSS 5.2 3.3 COD 1 47.0 24.0 Oil and grease 2.4 1.3 Phenolic compounds 0.056 0.027 Ammonia...

40 CFR 463.32 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... for the finishing water processes at a point source times the following pollutant concentrations: Subpart C [Finishing water] Concentration used to calculate BPT effluent limitations Pollutant or pollutant property Maximum for any 1 day (mg/l) Maximum for monthly average (mg/l) TSS 130 37 pH (1) (1) 1...

40 CFR 463.32 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... finishing water processes at a point source times the following pollutant concentrations: Subpart C [Finishing water] Concentration used to calculate BPT effluent limitations Pollutant or pollutant property Maximum for any 1 day (mg/l) Maximum for monthly average (mg/l) TSS 130 37 pH (1) (1) 1 Within the range...

40 CFR 463.32 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... for the finishing water processes at a point source times the following pollutant concentrations: Subpart C [Finishing water] Concentration used to calculate BPT effluent limitations Pollutant or pollutant property Maximum for any 1 day (mg/l) Maximum for monthly average (mg/l) TSS 130 37 pH (1) (1) 1...

40 CFR 463.32 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... for the finishing water processes at a point source times the following pollutant concentrations: Subpart C [Finishing water] Concentration used to calculate BPT effluent limitations Pollutant or pollutant property Maximum for any 1 day (mg/l) Maximum for monthly average (mg/l) TSS 130 37 pH (1) (1) 1...

Characterization of Transcription from TATA-Less Promoters: Identification of a New Core Promoter Element XCPE2 and Analysis of Factor Requirements

PubMed Central

Anish, Ramakrishnan; Hossain, Mohammad B.; Jacobson, Raymond H.; Takada, Shinako

2009-01-01

Background More than 80% of mammalian protein-coding genes are driven by TATA-less promoters which often show multiple transcriptional start sites (TSSs). However, little is known about the core promoter DNA sequences or mechanisms of transcriptional initiation for this class of promoters. Methodology/Principal Findings Here we identify a new core promoter element XCPE2 (X core promoter element 2) (consensus sequence: A/C/G-C-C/T-C-G/A-T-T-G/A-C-C/A+1-C/T) that can direct specific transcription from the second TSS of hepatitis B virus X gene mRNA. XCPE2 sequences can also be found in human promoter regions and typically appear to drive one of the start sites within multiple TSS-containing TATA-less promoters. To gain insight into mechanisms of transcriptional initiation from this class of promoters, we examined requirements of several general transcription factors by in vitro transcription experiments using immunodepleted nuclear extracts and purified factors. Our results show that XCPE2-driven transcription uses at least TFIIB, either TFIID or free TBP, RNA polymerase II (RNA pol II) and the MED26-containing mediator complex but not Gcn5. Therefore, XCPE2-driven transcription can be carried out by a mechanism which differs from previously described TAF-dependent mechanisms for initiator (Inr)- or downstream promoter element (DPE)-containing promoters, the TBP- and SAGA (Spt-Ada-Gcn5-acetyltransferase)-dependent mechanism for yeast TATA-containing promoters, or the TFTC (TBP-free-TAF-containing complex)-dependent mechanism for certain Inr-containing TATA-less promoters. EMSA assays using XCPE2 promoter and purified factors further suggest that XCPE2 promoter recognition requires a set of factors different from those for TATA box, Inr, or DPE promoter recognition. Conclusions/Significance We identified a new core promoter element XCPE2 that are found in multiple TSS-containing TATA-less promoters. Mechanisms of promoter recognition and transcriptional initiation for XCPE2-driven promoters appear different from previously shown mechanisms for classical promoters that show single “focused” TSSs. Our studies provide insight into novel mechanisms of RNA Pol II transcription from multiple TSS-containing TATA-less promoters. PMID:19337366

Coincident patterns of waste water suspended solids reduction, water transparency increase and chlorophyll decline in Narragansett Bay.

PubMed

Borkman, David G; Smayda, Theodore J

2016-06-15

Dramatic changes occurred in Narragansett Bay during the 1980s: water clarity increased, while phytoplankton abundance and chlorophyll concentration decreased. We examine how changes in total suspended solids (TSS) loading from wastewater treatment plants may have influenced this decline in phytoplankton chlorophyll. TSS loading, light and phytoplankton observations were compiled and a light- and temperature-dependent Skeletonema-based phytoplankton growth model was applied to evaluate chlorophyll supported by TSS nitrogen during 1983-1995. TSS loading declined 75% from ~0.60×10(6)kgmonth(-1) to ~0.15×10(6)kgmonth(-1) during 1983-1995. Model results indicate that nitrogen reduction related to TSS reduction was minor and explained a small fraction (~15%) of the long-term chlorophyll decline. The decline in NBay TSS loading appears to have increased water clarity and in situ irradiance and contributed to the long-term chlorophyll decline by inducing a physiological response of a ~20% reduction in chlorophyll per cell. Copyright © 2016 Elsevier Ltd. All rights reserved.

Effect of Toki-Shakuyaku-San on Regional Cerebral Blood Flow in Patients with Mild Cognitive Impairment and Alzheimer's Disease

PubMed Central

Matsuoka, Teruyuki; Narumoto, Jin; Shibata, Keisuke; Okamura, Aiko; Taniguchi, Shogo; Kitabayashi, Yurinosuke; Fukui, Kenji

2012-01-01

The aim of this study was to examine the effect of toki-shakuyaku-san (TSS) on mild cognitive impairment (MCI) and Alzheimer's disease (AD) using single-photon emission computed tomography (SPECT). All subjects were administered TSS (7.5 g/day) for eight weeks. SPECT and evaluations using the Mini Mental State Examination (MMSE), Neuropsychiatric Inventory, and Physical Self-Maintenance Scale were performed before and after treatment with TSS. Three patients with MCI and five patients with AD completed the study. No adverse events occurred during the study period. After treatment with TSS, regional cerebral blood flow (rCBF) in the posterior cingulate was significantly higher than that before treatment. No brain region showed a significant decrease in rCBF. TSS treatment also tended to improve the score for orientation to place on the MMSE. These results suggest that TSS could be useful for treatment of MCI and AD. PMID:22454658

Optimization and validation of indirect ELISA using truncated TssB protein for the serodiagnosis of glanders amongst equines.

PubMed

Singha, Harisankar; Malik, Praveen; Goyal, Sachin K; Khurana, Sandip K; Mukhopadhyay, Chiranjay; Eshwara, Vandana K; Singh, Raj K

2014-01-01

To express truncated TssB protein of Burkholderia mallei and to evaluate its diagnostic efficacy for serological detection of glanders among equines. In an attempt to develop recombinant protein based enzyme-linked immunosorbent assay (ELISA), N-terminal 200 amino acid sequences of B. mallei TssB protein-a type 6 secretory effector protein--were expressed in prokaryotic expression system. Diagnostic potential of recombinant TssB protein was evaluated in indirect ELISA using a panel of glanders positive (n = 49), negative (n = 30), and field serum samples (n = 1811). Cross-reactivity of the assay was assessed with equine disease control serum and human melioidosis positive serum. In comparison to CFT, diagnostic sensitivity and specificity of ELISA were 99.7% and 100%, respectively. The indirect ELISA method using the truncated TssB offered safer and more rapid and efficient means of serodiagnosis of glanders in equines. These data highlight the use of TssB as potential diagnostic antigen for serological diagnosis of glanders.

Awareness of the Tuskegee syphilis study: impact on offenders' decisions to decline research participation.

PubMed

Poythress, Norman; Epstein, Monica; Stiles, Paul; Edens, John F

2011-01-01

Ethnic and racial minorities are often under-represented in research. There is considerable speculation that Blacks, in particular, are discouraged from research participation because of researcher improprieties in the U.S. Public Health Service (USPHS) Syphilis Study at Tuskegee (aka the Tuskegee Syphilis Study [TSS]), a 40-year (1932-1972) study in which investigators withheld medical treatment from African-American men infected with syphilis. We debriefed 281 offenders who declined participation in a research study to assess the extent to which knowledge of the TSS impacted their decisions not to enroll. Relatively few (44/281; 15.6%) reported awareness of the TSS. Half (n = 22) of these "aware" individuals could cite factually accurate information about the TSS, and only four individuals indicated that awareness of TSS had "somewhat" influenced their decision to not participate. Findings suggest that the legacy of the TSS played a relatively minor role in these offenders' decisions to decline research participation. 2011 John Wiley & Sons, Ltd.

Influence of atypical retardation pattern on the peripapillary retinal nerve fibre distribution assessed by scanning laser polarimetry and optical coherence tomography.

PubMed

Schrems, W A; Laemmer, R; Hoesl, L M; Horn, F K; Mardin, C Y; Kruse, F E; Tornow, R P

2011-10-01

To investigate the influence of atypical retardation pattern (ARP) on the distribution of peripapillary retinal nerve fibre layer (RNFL) thickness measured with scanning laser polarimetry in healthy individuals and to compare these results with RNFL thickness from spectral domain optical coherence tomography (OCT) in the same subjects. 120 healthy subjects were investigated in this study. All volunteers received detailed ophthalmological examination, GDx variable corneal compensation (VCC) and Spectralis-OCT. The subjects were divided into four subgroups according to their typical scan score (TSS): very typical with TSS=100, typical with 99 ≥ TSS ≥ 91, less typical with 90 ≥ TSS ≥ 81 and atypical with TSS ≤ 80. Deviations from very typical normal values were calculated for 32 sectors for each group. There was a systematic variation of the RNFL thickness deviation around the optic nerve head in the atypical group for the GDxVCC results. The highest percentage deviation of about 96% appeared temporal with decreasing deviation towards the superior and inferior sectors, and nasal sectors exhibited a deviation of 30%. Percentage deviations from very typical RNFL values decreased with increasing TSS. No systematic variation could be found if the RNFL thickness deviation between different TSS-groups was compared with the OCT results. The ARP has a major impact on the peripapillary RNFL distribution assessed by GDx VCC; thus, the TSS should be included in the standard printout.

Human Leukocyte Antigen Class II Transgenic Mouse Model Unmasks the Significant Extrahepatic Pathology in Toxic Shock Syndrome

PubMed Central

Tilahun, Ashenafi Y.; Marietta, Eric V.; Wu, Tsung-Teh; Patel, Robin; David, Chella S.; Rajagopalan, Govindarajan

2011-01-01

Among the exotoxins produced by Staphylococcus aureus and Streptococcus pyogenes, the superantigens (SAgs) are the most potent T-cell activators known to date. SAgs are implicated in several serious diseases including toxic shock syndrome (TSS), Kawasaki disease, and sepsis. However, the immunopathogenesis of TSS and other diseases involving SAgs are still not completely understood. The commonly used conventional laboratory mouse strains do not respond robustly to SAgs in vivo. Therefore, they must be artificially rendered susceptible to TSS by using sensitizing agents such as d-galactosamine (d-galN), which skews the disease exclusively to the liver and, hence, is not representative of the disease in humans. SAg-induced TSS was characterized using transgenic mice expressing HLA class II molecules that are extremely susceptible to TSS without d-galN. HLA-DR3 transgenic mice recapitulated TSS in humans with extensive multiple-organ inflammation affecting the lung, liver, kidneys, heart, and small intestines. Heavy infiltration with T lymphocytes (both CD4+ and CD8+), neutrophils, and macrophages was noted. In particular, the pathologic changes in the small intestines were extensive and accompanied by significantly altered absorptive functions of the enterocytes. In contrast to massive liver failure alone in the d-galN sensitization model of TSS, findings of the present study suggest that gut dysfunction might be a key pathogenic event that leads to high morbidity and mortality in humans with TSS. PMID:21641398

Knowledge and Self-Perceived Risk for Toxic Shock Syndrome.

ERIC Educational Resources Information Center

Colbry, Sheila Lynds; Boutsen, Frank R.

1993-01-01

Survey investigated female college students' knowledge about toxic shock syndrome (TSS), noting relationships between knowledge, self-perceived risk of developing TSS, and menstrual product use. Data analysis indicated knowledge about TSS was inadequate, particularly among young females. Neither risk perception nor product use were mediated by…

The effects of stream crossings on total suspended sediment in North Carolina Piedmont forests

Treesearch

Johnny Boggs; Ge Sun; Steve McNulty

2017-01-01

This study determined total suspended sediment (TSS) at six stream crossings that represented a range of site conditions and forest operations in the Piedmont of North Carolina. Two wood and three steel bridgemats and one culvert were installed to cross the streams. The road classes for the crossings included four temporary skid trails and two permanent forest haul...

Special Advanced Studies for Pollution Prevention. Delivery Order 0058: The Monitor - Winter 2000

DTIC Science & Technology

2001-04-01

Burning/Open Detonation of Energetic Materials ➨Emission factors from a draft EPA report are incorporated into the guidance Site Restoration ➨Method...Aqueous Cleaner Recycle System Microfiltration Removes oil/grease & TSS from alkaline and acid cleaning baths Commodore Separation Technologies, Inc... Microfiltration Removes all heavy metals from wastewater and recycles water Infinity Chemicals Group Infinity Prep-L Deoxidizing Chemical

Small RNAs Targeting Transcription Start Site Induce Heparanase Silencing through Interference with Transcription Initiation in Human Cancer Cells

PubMed Central

Pu, Jiarui; Mei, Hong; Zhao, Jun; Huang, Kai; Zeng, Fuqing; Tong, Qiangsong

2012-01-01

Heparanase (HPA), an endo-h-D-glucuronidase that cleaves the heparan sulfate chain of heparan sulfate proteoglycans, is overexpressed in majority of human cancers. Recent evidence suggests that small interfering RNA (siRNA) induces transcriptional gene silencing (TGS) in human cells. In this study, transfection of siRNA against −9/+10 bp (siH3), but not −174/−155 bp (siH1) or −134/−115 bp (siH2) region relative to transcription start site (TSS) locating at 101 bp upstream of the translation start site, resulted in TGS of heparanase in human prostate cancer, bladder cancer, and gastric cancer cells in a sequence-specific manner. Methylation-specific PCR and bisulfite sequencing revealed no DNA methylation of CpG islands within heparanase promoter in siH3-transfected cells. The TGS of heparanase did not involve changes of epigenetic markers histone H3 lysine 9 dimethylation (H3K9me2), histone H3 lysine 27 trimethylation (H3K27me3) or active chromatin marker acetylated histone H3 (AcH3). The regulation of alternative splicing was not involved in siH3-mediated TGS. Instead, siH3 interfered with transcription initiation via decreasing the binding of both RNA polymerase II and transcription factor II B (TFIIB), but not the binding of transcription factors Sp1 or early growth response 1, on the heparanase promoter. Moreover, Argonaute 1 and Argonaute 2 facilitated the decreased binding of RNA polymerase II and TFIIB on heparanase promoter, and were necessary in siH3-induced TGS of heparanase. Stable transfection of the short hairpin RNA construct targeting heparanase TSS (−9/+10 bp) into cancer cells, resulted in decreased proliferation, invasion, metastasis and angiogenesis of cancer cells in vitro and in athymic mice models. These results suggest that small RNAs targeting TSS can induce TGS of heparanase via interference with transcription initiation, and significantly suppress the tumor growth, invasion, metastasis and angiogenesis of cancer cells. PMID:22363633

Sensitivity and Specificity of Double-Track Sign in the Detection of Transverse Sinus Stenosis: A Multicenter Retrospective Study

PubMed Central

Zhu, De-Sheng; Fu, Jue; Zhang, Yi; Xie, Chong; Wang, Xiao-Qing; Zhang, Yue; Yang, Jie; Li, Shi-Xu; Liu, Xiao-Bei; Wan, Zhi-Wen; Dong, Qiang; Guan, Yang-Tai

2015-01-01

Background Transverse sinus stenosis (TSS) is common among patients with cerebral venous sinus thrombosis. No previous studies have reported on double-track sign detected on axial Gd-enhanced T1WI in TSS. This study aimed to determine the sensitivity and specificity of the double-track sign in the detection of TSS. Methods We retrospectively reviewed medical records of 383 patients with transverse sinus thrombosis (TST) and 30 patients with normal transverse sinus from 5 participating hospitals in china from January 2008 to June 2014. 167 feasible transverse sinuses included in this study were categorized into TSS (n = 76), transverse sinus occlusion (TSO) (n = 52) and transverse sinus normal (TSN) groups (n = 39) according to imaging diagnosis on digital subtraction angiography (DSA) or magnetic resonance venography (MRV). Double-track sign on axial Gd-enhanced T1WI was compared among the three groups. Sensitivity and specificity of double-track sign in detection of TSS were calculated, with final imaging diagnosis of TSS on DSA or MRV as the reference standard. Results Of 383 patients with TST recruited over a 6.5-year period, 128 patients were enrolled in the study, 255 patients were excluded because of insufficient clinical data, imaging finding and delay time, and 30 matched patients with normal transverse sinus were enrolled in the control group. Therefore, double-track sign assessment was conducted in 167 available transverse sinuses of 158 patients. Of the 76 sinuses in TSS group, 51 had double-track sign. Of the other 91 sinuses in TSO and TSN groups, 3 had a false-positive double-track sign. Thus, double-track sign on axial Gd-enhanced T1WI was 67.1% (95% CI 55.3–77.2) sensitive and 96.7% (95% CI 89.9–99.1) specific for detection of TSS. Conclusions The double-track sign on axial Gd-enhanced T1WI is highly specific and moderate sensitive for detection of TSS. Nevertheless, it could be a direct sign and might provide an early clue for TSS. PMID:26291452

Sensitivity and Specificity of Double-Track Sign in the Detection of Transverse Sinus Stenosis: A Multicenter Retrospective Study.

PubMed

Zhu, De-Sheng; Fu, Jue; Zhang, Yi; Xie, Chong; Wang, Xiao-Qing; Zhang, Yue; Yang, Jie; Li, Shi-Xu; Liu, Xiao-Bei; Wan, Zhi-Wen; Dong, Qiang; Guan, Yang-Tai

2015-01-01

Transverse sinus stenosis (TSS) is common among patients with cerebral venous sinus thrombosis. No previous studies have reported on double-track sign detected on axial Gd-enhanced T1WI in TSS. This study aimed to determine the sensitivity and specificity of the double-track sign in the detection of TSS. We retrospectively reviewed medical records of 383 patients with transverse sinus thrombosis (TST) and 30 patients with normal transverse sinus from 5 participating hospitals in china from January 2008 to June 2014. 167 feasible transverse sinuses included in this study were categorized into TSS (n = 76), transverse sinus occlusion (TSO) (n = 52) and transverse sinus normal (TSN) groups (n = 39) according to imaging diagnosis on digital subtraction angiography (DSA) or magnetic resonance venography (MRV). Double-track sign on axial Gd-enhanced T1WI was compared among the three groups. Sensitivity and specificity of double-track sign in detection of TSS were calculated, with final imaging diagnosis of TSS on DSA or MRV as the reference standard. Of 383 patients with TST recruited over a 6.5-year period, 128 patients were enrolled in the study, 255 patients were excluded because of insufficient clinical data, imaging finding and delay time, and 30 matched patients with normal transverse sinus were enrolled in the control group. Therefore, double-track sign assessment was conducted in 167 available transverse sinuses of 158 patients. Of the 76 sinuses in TSS group, 51 had double-track sign. Of the other 91 sinuses in TSO and TSN groups, 3 had a false-positive double-track sign. Thus, double-track sign on axial Gd-enhanced T1WI was 67.1% (95% CI 55.3-77.2) sensitive and 96.7% (95% CI 89.9-99.1) specific for detection of TSS. The double-track sign on axial Gd-enhanced T1WI is highly specific and moderate sensitive for detection of TSS. Nevertheless, it could be a direct sign and might provide an early clue for TSS.

Sampling Methodologies for Epidemiologic Surveillance of Men Who Have Sex with Men and Transgender Women in Latin America: An Empiric Comparison of Convenience Sampling, Time Space Sampling, and Respondent Driven Sampling

PubMed Central

Clark, J. L.; Konda, K. A.; Silva-Santisteban, A.; Peinado, J.; Lama, J. R.; Kusunoki, L.; Perez-Brumer, A.; Pun, M.; Cabello, R.; Sebastian, J. L.; Suarez-Ognio, L.; Sanchez, J.

2014-01-01

Alternatives to convenience sampling (CS) are needed for HIV/STI surveillance of most-at-risk populations in Latin America. We compared CS, time space sampling (TSS), and respondent driven sampling (RDS) for recruitment of men who have sex with men (MSM) and transgender women (TW) in Lima, Peru. During concurrent 60-day periods from June–August, 2011, we recruited MSM/TW for epidemiologic surveillance using CS, TSS, and RDS. A total of 748 participants were recruited through CS, 233 through TSS, and 127 through RDS. The TSS sample included the largest proportion of TW (30.7 %) and the lowest percentage of subjects who had previously participated in HIV/STI research (14.9 %). The prevalence of newly diagnosed HIV infection, according to participants’ self-reported previous HIV diagnosis, was highest among TSS recruits (17.9 %) compared with RDS (12.6 %) and CS (10.2 %). TSS identified diverse populations of MSM/TW with higher prevalences of HIV/STIs not accessed by other methods. PMID:24362754

Sampling methodologies for epidemiologic surveillance of men who have sex with men and transgender women in Latin America: an empiric comparison of convenience sampling, time space sampling, and respondent driven sampling.

PubMed

Clark, J L; Konda, K A; Silva-Santisteban, A; Peinado, J; Lama, J R; Kusunoki, L; Perez-Brumer, A; Pun, M; Cabello, R; Sebastian, J L; Suarez-Ognio, L; Sanchez, J

2014-12-01

Alternatives to convenience sampling (CS) are needed for HIV/STI surveillance of most-at-risk populations in Latin America. We compared CS, time space sampling (TSS), and respondent driven sampling (RDS) for recruitment of men who have sex with men (MSM) and transgender women (TW) in Lima, Peru. During concurrent 60-day periods from June-August, 2011, we recruited MSM/TW for epidemiologic surveillance using CS, TSS, and RDS. A total of 748 participants were recruited through CS, 233 through TSS, and 127 through RDS. The TSS sample included the largest proportion of TW (30.7 %) and the lowest percentage of subjects who had previously participated in HIV/STI research (14.9 %). The prevalence of newly diagnosed HIV infection, according to participants' self-reported previous HIV diagnosis, was highest among TSS recruits (17.9 %) compared with RDS (12.6 %) and CS (10.2 %). TSS identified diverse populations of MSM/TW with higher prevalences of HIV/STIs not accessed by other methods.

40 CFR 417.156 - Pretreatment standards for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... be no discharge of waste water streams in which both the COD/BOD7 ratio exceeds 10.0 and the COD... when a high rate of wet scrubbing is in operation which produces more waste water than can be recycled... No limitations. COD Do. TSS Do. Surfactants Do. Oil and grease Do. pH Do. (3) For fast turnaround...

40 CFR 426.115 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... (the fluoride and lead limitations are applicable to the abrasive polishing and acid polishing waste water streams while the TSS, oil, and pH limitations are applicable to the entire process waste water... 130.0 Fluoride 120.0 60.0 Lead 30.9 0.45 pH (1) (1) English units (lb/1,000 lb of furnace pull) Oil 0...

40 CFR 426.115 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... (the fluoride and lead limitations are applicable to the abrasive polishing and acid polishing waste water streams while the TSS, oil, and pH limitations are applicable to the entire process waste water... 130.0 Fluoride 120.0 60.0 Lead 30.9 0.45 pH (1) (1) English units (lb/1,000 lb of furnace pull) Oil 0...

40 CFR 426.115 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... (the fluoride and lead limitations are applicable to the abrasive polishing and acid polishing waste water streams while the TSS, oil, and pH limitations are applicable to the entire process waste water... 130.0 Fluoride 120.0 60.0 Lead 30.9 0.45 pH (1) (1) English units (lb/1,000 lb of furnace pull) Oil 0...

40 CFR 426.115 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (the fluoride and lead limitations are applicable to the abrasive polishing and acid polishing waste water streams while the TSS, oil, and pH limitations are applicable to the entire process waste water... 130.0 Fluoride 120.0 60.0 Lead 30.9 0.45 pH (1) (1) English units (lb/1,000 lb of furnace pull) Oil 0...

40 CFR 423.15 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... exceed (mg/l) TSS 100.0 30.0 Oil and grease 20.0 15.0 Copper, total 1.0 1.0 Iron, total 1.0 1.0 (e... pollutants from fly ash transport water. (h)(1) For any plant with a total rated electric generating capacity... or pollutant property NSPS effluent limitations Maximum concentration (mg/l) Total residual chlorine...

40 CFR 471.83 - New source performance standards (NSPS).

Code of Federal Regulations, 2012 CFR

2012-07-01

... all times. (j) Alkaline cleaning spent baths. Subpart H—NSPS Pollutant or pollutant property Maximum for any 1 day Maximum for monthly average mg/off-kg (pounds per million off-pounds) of zinc alkaline....036 0.036 TSS 0.054 0.043 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (k) Alkaline...

40 CFR 471.83 - New source performance standards (NSPS).

Code of Federal Regulations, 2014 CFR

2014-07-01

... all times. (j) Alkaline cleaning spent baths. Subpart H—NSPS Pollutant or pollutant property Maximum for any 1 day Maximum for monthly average mg/off-kg (pounds per million off-pounds) of zinc alkaline....036 0.036 TSS 0.054 0.043 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (k) Alkaline...

40 CFR 471.83 - New source performance standards (NSPS).

Code of Federal Regulations, 2013 CFR

2013-07-01

... all times. (j) Alkaline cleaning spent baths. Subpart H—NSPS Pollutant or pollutant property Maximum for any 1 day Maximum for monthly average mg/off-kg (pounds per million off-pounds) of zinc alkaline....036 0.036 TSS 0.054 0.043 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (k) Alkaline...

40 CFR 471.43 - New source performance standards (NSPS).

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Alkaline cleaning spent baths. Subpart D—NSPS Pollutant or pollutant property Maximum for any 1 day Maximum for monthly average mg/off-kg (pounds per million off-pounds) of precious metals alkaline cleaned... TSS 2.46 1.17 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (q) Alkaline cleaning rinse...

40 CFR 471.63 - New source performance standards (NSPS).

Code of Federal Regulations, 2013 CFR

2013-07-01

... times. (p) Alkaline cleaning spent baths. Subpart F—NSPS Pollutant or pollutant property Maximum for any 1 day Maximum for monthly average mg/off-kg (pounds per million off-pounds) of titanium alkaline... grease 4.80 2.88 TSS 9.84 4.68 pH (1) (1) 1 Within the range of 7.5 to 10.0 at all times. (q) Alkaline...

40 CFR 464.42 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS METAL MOLDING AND CASTING POINT SOURCE CATEGORY Zinc... for copper, lead, zinc, total phenols, oil and grease, and TSS. For non-continuous dischargers, annual... metal poured Copper (T) 0.0344 0.0187 Lead (T) 0.0353 0.0174 Zinc (T) 0.0509 0.0192 Oil and grease 1.34...

40 CFR 464.42 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS METAL MOLDING AND CASTING POINT SOURCE CATEGORY Zinc... for copper, lead, zinc, total phenols, oil and grease, and TSS. For non-continuous dischargers, annual... metal poured Copper (T) 0.0344 0.0187 Lead (T) 0.0353 0.0174 Zinc (T) 0.0509 0.0192 Oil and grease 1.34...

40 CFR 419.16 - Standards of performance for new sources (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... (kilograms per cubic meter of flow) BOD5 11.8 6.3 TSS 8.3 4.9 COD 1 61.0 32 Oil and grease 3.6 1.9 Phenolic... 21.7 11.2 Oil and grease 1.3 0.70 Phenolic compounds 0.031 0.016 Ammonia as N 1.0 0.45 Sulfide 0.027...

40 CFR 426.116 - Pretreatment standards for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... the recognition that animal and vegetable oils can be adequately removed in a publicly owned treatment... (animal and vegetable) (1) (1) Oil (mineral) 260.0 130.0 TSS (1) (1) Fluoride 120.0 60.0 Lead (1) (1) pH (1) (1) English units (lb/1,000 lb of furnace pull) Oil (animal and vegetable) (1) (1) Oil 0.26 0.13...

40 CFR 426.116 - Pretreatment standards for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... the recognition that animal and vegetable oils can be adequately removed in a publicly owned treatment... (animal and vegetable) (1) (1) Oil (mineral) 260.0 130.0 TSS (1) (1) Fluoride 120.0 60.0 Lead (1) (1) pH (1) (1) English units (lb/1,000 lb of furnace pull) Oil (animal and vegetable) (1) (1) Oil 0.26 0.13...

40 CFR 463.17 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... contact cooling and heating water processes at a point source times the following pollutant concentrations: Subpart A [Contact cooling and heating water] Concentration used to calculate BCT effluent limitations Pollutant or pollutant property Maximum for any 1 day (mg/l) BOD5 26 Oil and grease 29 TSS 19 pH (1) 1...

40 CFR 463.17 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... contact cooling and heating water processes at a point source times the following pollutant concentrations: Subpart A [Contact cooling and heating water] Concentration used to calculate BCT effluent limitations Pollutant or pollutant property Maximum for any 1 day (mg/l) BOD5 26 Oil and grease 29 TSS 19 pH (1) 1...

40 CFR 463.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... TSS 117 36 pH (1) (1) 1 Within the range of 6.0 to 9.0 at all times. The permit authority will obtain... for the cleaning water processes at a point source times the following pollutant concentrations: Subpart B [Cleaning water] Concentration used to calculate BPT effluent limitations Pollutant or pollutant...

40 CFR 463.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... TSS 117 36 pH (1) (1) 1 Within the range of 6.0 to 9.0 at all times. The permit authority will obtain... for the cleaning water processes at a point source times the following pollutant concentrations: Subpart B [Cleaning water] Concentration used to calculate BPT effluent limitations Pollutant or pollutant...

40 CFR 463.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... TSS 117 36 pH (1) (1) 1 Within the range of 6.0 to 9.0 at all times. The permit authority will obtain... for the cleaning water processes at a point source times the following pollutant concentrations: Subpart B [Cleaning water] Concentration used to calculate BPT effluent limitations Pollutant or pollutant...

40 CFR 463.17 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... contact cooling and heating water processes at a point source times the following pollutant concentrations: Subpart A [Contact cooling and heating water] Concentration used to calculate BCT effluent limitations Pollutant or pollutant property Maximum for any 1 day (mg/l) BOD5 26 Oil and grease 29 TSS 19 pH (1) 1...

40 CFR 463.17 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... cooling and heating water processes at a point source times the following pollutant concentrations: Subpart A [Contact cooling and heating water] Concentration used to calculate BCT effluent limitations Pollutant or pollutant property Maximum for any 1 day (mg/l) BOD5 26 Oil and grease 29 TSS 19 pH (1) 1...

Bistatic scattering from submerged unexploded ordnance lying on a sediment.

PubMed

Bucaro, J A; Simpson, H; Kraus, L; Dragonette, L R; Yoder, T; Houston, B H

2009-11-01

The broadband bistatic target strengths (TSs) of two submerged unexploded ordnance (UXO) targets have been measured in the NRL sediment pool facility. The targets-a 5 in. rocket and a 155 mm projectile-were among the targets whose monostatic TSs were measured and reported previously by the authors. Bistatic TS measurements were made for 0 degrees (target front) and 90 degrees (target side) incident source directions, and include both backscattered and forward scattered echo angles over a complete 360 degrees with the targets placed proud of the sediment surface. For the two source angles used, each target exhibits two strong highlights: a backscattered specular-like echo and a forward scattered response. The TS levels of the former are shown to agree reasonably well with predictions, based on scattering from rigid disks and cylinders, while the levels of the latter with predictions from radar cross section models, based on simple geometric optics appropriately modified. The bistatic TS levels observed for the proud case provide comparable or higher levels of broadband TS relative to free-field monostatic measurements. It is concluded that access to bistatic echo information in operations aimed at detecting submerged UXO targets could provide an important capability.

Prevalence, comorbidities and mortality of toxic shock syndrome in children and adults in the USA.

PubMed

Strom, Mark A; Hsu, Derek Y; Silverberg, Jonathan I

2017-11-01

Toxic Shock Syndrome (TSS), a superantigen-mediated illness, is characterized by rash, hypotension and multi-organ dysfunction. Predictors of TSS and related morbidity and mortality are poorly defined. In this study, data on 61,959,084 hospitalizations from the 2003-2012 Nationwide Inpatient Sample, a 20% stratified sample of US hospitalizations, were analyzed and ICD-9-CM coding used to identify 4491 hospitalizations with a diagnosis of TSS. Incidence, in-hospital mortality rate, comorbidities, length of stay and costs of care attributable to TSS were determined. In multivariate survey logistic regression models, TSS was associated with female sex (adjusted odds ratio [95% confidence interval], 1.54 [1.48-1.60]), younger age (0-17 years, 2.17 [2.06-2.29]; 40-59: 0.53 [0.50-0.56]; 60-79: 0.28 [0.26-0.30]; 80+: 0.13 [0.11-0.14] compared with 18-39) and race/ethnicity (black, 0.63 [0.59-0.67]; Hispanic: 0.60 [0.56-0.64]; Asian, 1.11 [1.00-1.11]; and other, 0.83 [0.75-0.92] compared with white). Patients with TSS had a three-fold greater cost of care (mean: $36,656 ± 942) and length of stay (LOS) (mean: 10.65 ± 0.23 days) than patients without TSS. Shared predictors of increased LOS and costs in patients with TSS were male sex; age 40-79 years; Black, Hispanic, Asian and other race/ethnicity; and more than one chronic condition. Predictors of in-hospital mortality included respiratory failure (13.66 [11.37-16.43]), liver disease/failure (3.36 [2.59-4.34]), chickenpox (91.26 [27.74-300.25]), coagulopathy (2.14 [1.85-2.48]), and higher age. In conclusion, there are significant racial/ethnic, socioeconomic, and comorbid disparities in the incidence and mortality of TSS in adults and children in the USA. © 2017 The Societies and John Wiley & Sons Australia, Ltd.

Glaucoma diagnostic performance of GDxVCC and spectralis OCT on eyes with atypical retardation pattern.

PubMed

Hoesl, Laura Maria; Tornow, Ralf P; Schrems, Wolfgang A; Horn, Folkert K; Mardin, Christian Y; Kruse, Friedrich E; Juenemann, Anselm G M; Laemmer, Robert

2013-01-01

To investigate the impact of typical scan score (TSS) on discriminating glaucomatous and healthy eyes by scanning laser polarimetry and spectral domain optical coherence tomography (SD-OCT) in 32 peripapillary sectors. One hundred two glaucoma patients and 32 healthy controls underwent standard automated perimetry, 24-hour intraocular pressure profile, optic disc photography, GDxVCC, and SD-OCT measurements. For controls, only very typical scans (TSS=100) were accepted. Glaucoma patients were divided into 3 subgroups (very typical: TSS=100; typical: 99≥TSS≥80, atypical: TSS<80). Receiver operating characteristic curves were constructed for mean retinal nerve fiber layer values, sector data, and nerve fiber indicator (NFI). Sensitivity was estimated at ≥90% specificity to compare the discriminating ability of each imaging modality. For discrimination between healthy and glaucomatous eyes with very typical scans, the NFI and inferior sector analyses 26 to 27 demonstrated the highest sensitivity at ≥90% specificity in GDxVCC and SD-OCT, respectively. For the typical and atypical groups, sensitivity at ≥90% specificity decreased for all 32 peripapillary sectors on an average by 10.9% and 17.9% for GDxVCC and by 4.9% and 0.8% for SD-OCT. For GDxVCC, diagnostic performance of peripapillary sectors decreased with lower TSS, especially in temporosuperior and inferotemporal sectors (sensitivity at ≥90% specificity decreased by 55.3% and by 37.8% in the atypical group). Diagnostic accuracy is comparable for SD-OCT and GDxVCC if typical scans (TSS=100) are investigated. Decreasing TSS is associated with a decrease in diagnostic accuracy for discriminating healthy and glaucomatous eyes by scanning laser polarimetry. NFI is less influenced than the global or sector retinal nerve fiber layer thickness. The TSS score should be included in the standard printout. Diagnostic accuracy of SD-OCT is barely influenced by low TSS.

Coevolution of the ATPase ClpV, the Sheath Proteins TssB and TssC, and the Accessory Protein TagJ/HsiE1 Distinguishes Type VI Secretion Classes*

PubMed Central

Förster, Andreas; Planamente, Sara; Manoli, Eleni; Lossi, Nadine S.; Freemont, Paul S.; Filloux, Alain

2014-01-01

The type VI secretion system (T6SS) is a bacterial nanomachine for the transport of effector molecules into prokaryotic and eukaryotic cells. It involves the assembly of a tubular structure composed of TssB and TssC that is similar to the tail sheath of bacteriophages. The sheath contracts to provide the energy needed for effector delivery. The AAA+ ATPase ClpV disassembles the contracted sheath, which resets the systems for reassembly of an extended sheath that is ready to fire again. This mechanism is crucial for T6SS function. In Vibrio cholerae, ClpV binds the N terminus of TssC within a hydrophobic groove. In this study, we resolved the crystal structure of the N-terminal domain of Pseudomonas aeruginosa ClpV1 and observed structural alterations in the hydrophobic groove. The modification in the ClpV1 groove is matched by a change in the N terminus of TssC, suggesting the existence of distinct T6SS classes. An accessory T6SS component, TagJ/HsiE, exists predominantly in one of the classes. Using bacterial two-hybrid approaches, we showed that the P. aeruginosa homolog HsiE1 interacts strongly with ClpV1. We then resolved the crystal structure of HsiE1 in complex with the N terminus of HsiB1, a TssB homolog and component of the contractile sheath. Phylogenetic analysis confirmed that these differences distinguish T6SS classes that resulted from a functional co-evolution between TssB, TssC, TagJ/HsiE, and ClpV. The interaction of TagJ/HsiE with the sheath as well as with ClpV suggests an alternative mode of disassembly in which HsiE recruits the ATPase to the sheath. PMID:25305017

Managing toxic shock syndrome with antibiotics.

PubMed

Annane, Djillali; Clair, Bernard; Salomon, Jérôme

2004-08-01

Toxic shock syndrome (TSS) is a serious disorder with a worldwide prevalence of approximately 3/100,000 persons. TSS is mainly caused by Streptococcus pyogenes or Staphylococcus aureus. Thus, beta-lactam and lincosamides, such as clindamycin, are the first-line drugs. Yet, the mortality rate remains unacceptably high; highlighting the role of bacterial toxin-mediated activation of the inflammatory cascade in TSS pathogenesis. Further strategies should be targeted towards interfering with the interaction between bacterial toxins and host T cells. This paper aims to provide an overview of the epidemiology, pathomechanisms, and clinical presentation of TSS, and criteria for selecting drugs among available antibiotics.

Determinants of G quadruplex-induced epigenetic instability in REV1-deficient cells

PubMed Central

Schiavone, Davide; Guilbaud, Guillaume; Murat, Pierre; Papadopoulou, Charikleia; Sarkies, Peter; Prioleau, Marie-Noëlle; Balasubramanian, Shankar; Sale, Julian E

2014-01-01

REV1-deficient chicken DT40 cells are compromised in replicating G quadruplex (G4)-forming DNA. This results in localised, stochastic loss of parental chromatin marks and changes in gene expression. We previously proposed that this epigenetic instability arises from G4-induced replication fork stalls disrupting the accurate propagation of chromatin structure through replication. Here, we test this model by showing that a single G4 motif is responsible for the epigenetic instability of the BU-1 locus in REV1-deficient cells, despite its location 3.5 kb from the transcription start site (TSS). The effect of the G4 is dependent on it residing on the leading strand template, but is independent of its in vitro thermal stability. Moving the motif to more than 4 kb from the TSS stabilises expression of the gene. However, loss of histone modifications (H3K4me3 and H3K9/14ac) around the transcription start site correlates with the position of the G4 motif, expression being lost only when the promoter is affected. This supports the idea that processive replication is required to maintain the histone modification pattern and full transcription of this model locus. PMID:25190518

21 CFR 801.430 - User labeling for menstrual tampons.

Code of Federal Regulations, 2010 CFR

2010-04-01

... risk of TSS to all women using tampons during their menstrual period, especially the reported higher risks to women under 30 years of age and teenage girls, the estimated incidence of TSS of 1 to 17 per 100,000 menstruating women and girls per year, and the risk of death from contracting TSS; (3) The...

Characterization of the Truncated Androgen Receptor Generated by Calpain-Dependent Proteolysis in Prostate Cancer

DTIC Science & Technology

2009-08-31

hierarchical network of transcription factors governs androgen receptor-dependent prostate cancer growth. Mol Cell, 2007. 27(3): p. 380 -92. 26. Takayama, K...TSS_upstream * 69570641 69571326 0.02 UGT2B15 -5 TSS_upstream 71417985 71418195 0.05 UNQ689 -796 TSS_upstream 29 83631386 83632082 0.02 MASA 60948

Tanshinone IIA Sodium Sulfonate Attenuates LPS-Induced Intestinal Injury in Mice

PubMed Central

Yang, Xin-Jing; Qian, Jin-Xian; Wei, Yao; Guo, Qiang; Jin, Jun; Sun, Xue; Liu, Sheng-Lan

2018-01-01

Background Tanshinone IIA sodium sulfonate (TSS) is known to possess anti-inflammatory effects and has exhibited protective effects in various inflammatory conditions; however, its role in lipopolysaccharide- (LPS-) induced intestinal injury is still unknown. Objective The present study is designed to explore the role and possible mechanism of TSS in LPS-induced intestinal injury. Methods Male C57BL/6J mice, challenged with intraperitoneal LPS injection, were treated with or without TSS 0.5 h prior to LPS exposure. At 1, 6, and 12 h after LPS injection, mice were sacrificed, and the small intestine was excised. The intestinal tissue injury was analyzed by HE staining. Inflammatory factors (TNF-α, IL-1β, and IL-6) in the intestinal tissue were examined by ELISA and RT-PCR. In addition, expressions of autophagy markers (microtubule-associated light chain 3 (LC3) and Beclin-1) were detected by western blot and RT-PCR. A number of autophagosomes were also observed under electron microscopy. Results TSS treatment significantly attenuated small intestinal epithelium injury induced by LPS. LPS-induced release of inflammatory mediators, including TNF-α, IL-1β, and IL-6, were markedly inhibited by TSS. Furthermore, TSS treatment could effectively upregulate LPS-induced decrease of autophagy levels, as evidenced by the increased expression of LC3 and Beclin-1, and more autophagosomes. Conclusion The protective effect of TSS on LPS-induced small intestinal injury may be attributed to the inhibition of inflammatory factors and promotion of autophagy levels. The present study may provide novel insight into the molecular mechanisms of TSS on the treatment of intestinal injury. PMID:29706995

Transsphenoidal surgery and diabetes mellitus: An analysis of inpatient data and complications.

PubMed

Pines, Morgan J; Raikundalia, Milap D; Svider, Peter F; Baredes, Soly; Liu, James K; Eloy, Jean Anderson

2015-10-01

Transsphenoidal surgery (TSS) has emerged as the standard approach for pituitary resection due to its minimally invasive nature. There has been little analysis examining the impact of diabetes mellitus (DM) on patients undergoing TSS. In this study, we characterize DM's association with postoperative TSS complications. In addition to analysis of associated charges and patient demographics, we performed comparison of complication rates between DM and non-DM patients who have undergone TSS in recent years. The Nationwide Inpatient Sample, a database encompassing nearly 8 million inpatient hospitalizations, was evaluated for patients undergoing TSS from 2002 to 2010. Of 12,938 TSS patients, 2,173 (16.8%) had a DM diagnosis. The non-DM cohort was younger (50.1 y ± 16.6SD vs. 56.8 y ± 14.1; P < 0.001) and had shorter hospitalizations and lesser charges. DM patients had a greater incidence of pulmonary, cardiac, urinary/renal, and fluid/electrolyte complications, and had a lesser incidence of diabetes insipidus (P < 0.05). Upon controlling for age, the greater incidence of pulmonary and fluid/electrolyte complications was present only among patients < 60 years of age. Higher occurrence of cerebrospinal fluid rhinorrhea was noted among black diabetics when compared to non-DM blacks. DM is associated with greater length of stay and hospital charges among TSS patients. DM patients undergoing TSS have a significantly greater incidence of pulmonary and fluid/electrolyte complications among patients under the age of 60, and greater risk for urinary/renal complications across all ages. Despite a theoretical concern due to an impaired wound-healing in DM patients, association with cerebrospinal fluid rhinorrhea was only noted among black diabetics. 2C. © 2015 The American Laryngological, Rhinological and Otological Society, Inc.

Characterization of transsphenoidal complications in patients with acromegaly: an analysis of inpatient data in the United States from 2002 to 2010.

PubMed

Raikundalia, Milap D; Pines, Morgan J; Svider, Peter F; Baredes, Soly; Folbe, Adam J; Liu, James K; Eloy, Jean Anderson

2015-05-01

Transsphenoidal surgery (TSS) is a common procedure for a variety of pituitary lesions. This procedure can be associated with complications related to the surgery or specific pathology. In this study, we evaluate inpatient postoperative complications among patients who underwent TSS for growth hormone adenomas using a nationally representative database, and compare patient characteristics and complications to patients who underwent TSS for other benign pituitary neoplasms. Analysis of the Nationwide Inpatient Sample revealed 13,070 TSS patients (including 892 with acromegaly) between 2002 and 2010. Complication rates, outcomes, patient demographics, hospital stay, and total charges were evaluated among TSS patients with and without acromegaly. There was an increase in TSS performed in both cohorts from 2002 to 2010. Acromegaly patients were younger, had shorter hospital stays, and incurred fewer charges. Acromegaly patients had a lower occurrence of postoperative urinary/renal complications (0.2% vs 1.1%), thromboembolic events (0% vs 0.4%), fluid/electrolyte abnormalities (5.7% vs 9.1%), and iatrogenic hypopituitarism (0.3% vs 1.1%) compared to other TSS patients (all p < 0.05). After adjusting for age, acromegalic patients maintained a statistically lower occurrence of fluid/electrolyte abnormalities (p = 0.007). Cerebrospinal fluid leak occurrence in acromegaly patients was 2.6% vs 1.7% in non-acromegaly patients, a result that did not reach significance (p = 0.054). Upon comparison of inpatient hospitalizations for patients undergoing TSS for growth hormone adenomas and other benign pituitary neoplasms, acromegaly patients had a significantly lower occurrence of postoperative fluid/electrolyte abnormalities. Acromegaly patients had shorter hospitalizations and subsequently fewer total charges. © 2015 ARS-AAOA, LLC.

Distribution of CpG Motifs in Upstream Gene Domains in a Reef Coral and Sea Anemone: Implications for Epigenetics in Cnidarians.

PubMed

Marsh, Adam G; Hoadley, Kenneth D; Warner, Mark E

2016-01-01

Coral reefs are under assault from stressors including global warming, ocean acidification, and urbanization. Knowing how these factors impact the future fate of reefs requires delineating stress responses across ecological, organismal and cellular scales. Recent advances in coral reef biology have integrated molecular processes with ecological fitness and have identified putative suites of temperature acclimation genes in a Scleractinian coral Acropora hyacinthus. We wondered what unique characteristics of these genes determined their coordinate expression in response to temperature acclimation, and whether or not other corals and cnidarians would likewise possess these features. Here, we focus on cytosine methylation as an epigenetic DNA modification that is responsive to environmental stressors. We identify common conserved patterns of cytosine-guanosine dinucleotide (CpG) motif frequencies in upstream promoter domains of different functional gene groups in two cnidarian genomes: a coral (Acropora digitifera) and an anemone (Nematostella vectensis). Our analyses show that CpG motif frequencies are prominent in the promoter domains of functional genes associated with environmental adaptation, particularly those identified in A. hyacinthus. Densities of CpG sites in upstream promoter domains near the transcriptional start site (TSS) are 1.38x higher than genomic background levels upstream of -2000 bp from the TSS. The increase in CpG usage suggests selection to allow for DNA methylation events to occur more frequently within 1 kb of the TSS. In addition, observed shifts in CpG densities among functional groups of genes suggests a potential role for epigenetic DNA methylation within promoter domains to impact functional gene expression responses in A. digitifera and N. vectensis. Identifying promoter epigenetic sequence motifs among genes within specific functional groups establishes an approach to describe integrated cellular responses to environmental stress in reef corals and potential roles of epigenetics on survival and fitness in the face of global climate change.

A role for 11C-methionine PET imaging in ACTH-dependent Cushing's syndrome.

PubMed

Koulouri, Olympia; Steuwe, Andrea; Gillett, Daniel; Hoole, Andrew C; Powlson, Andrew S; Donnelly, Neil A; Burnet, Neil G; Antoun, Nagui M; Cheow, Heok; Mannion, Richard J; Pickard, John D; Gurnell, Mark

2015-10-01

We report our experience of functional imaging with (11)C-methionine positron emission tomography-computed tomography (PET-CT) co-registered with 3D gradient echo (spoiled gradient recalled (SPGR)) magnetic resonance imaging (MRI) in the investigation of ACTH-dependent Cushing's syndrome. Twenty patients with i) de novo Cushing's disease (CD, n=10), ii) residual or recurrent hypercortisolism following first pituitary surgery (±radiotherapy; n=8) or iii) ectopic Cushing's syndrome (n=2) were referred to our centre for functional imaging studies between 2010 and 2015. Six of the patients with de novo CD and five of those with persistent/relapsed disease had a suspected abnormality on conventional MRI. All patients underwent (11)C-methionine PET-CT. For pituitary imaging, co-registration of PET-CT images with contemporaneous SPGR MRI (1 mm slice thickness) was performed, followed by detailed mapping of (11)C-methionine uptake across the sella in three planes (coronal, sagittal and axial). This allowed us to determine whether suspected adenomas seen on structural imaging exhibited focal tracer uptake on functional imaging. In seven of ten patients with de novo CD, asymmetric (11)C-methionine uptake was observed within the sella, which co-localized with the suspected site of a corticotroph microadenoma visualised on SPGR MRI (and which was subsequently confirmed histologically following successful transsphenoidal surgery (TSS)). Focal (11)C-methionine uptake that correlated with a suspected abnormality on pituitary MRI was seen in five of eight patients with residual or recurrent Cushing's syndrome following first TSS (and pituitary radiotherapy in two cases). Two patients elected to undergo repeat TSS with histology confirming a corticotroph tumour in each case. In two patients with the ectopic ACTH syndrome, (11)C-methionine was concentrated in sites of distant metastases, with minimal uptake in the sellar region. (11)C-methionine PET-CT can aid the detection of ACTH-secreting tumours in Cushing's syndrome and facilitate targeted therapy. © 2015 European Society of Endocrinology.

Toxic shock syndrome post open reduction and Kirschner wire fixation of a humeral lateral condyle fracture

PubMed Central

Chan, Yuen; Selvaratnam, Veenesh; Garg, Neeraj

2015-01-01

Use of Kirschner wires (K-wires) is the most common method of fracture stabilisation in lateral condyle fracture fixation in children. We report a case of toxic shock syndrome (TSS) following an open reduction and internal fixation using K-wires for a humeral lateral condyle fracture in a 5-year-old girl. TSS is a toxin-mediated multisystem illness. It typically presents with shock and it is most often attributed to toxin-producing strains of Staphylococcus aureus and Streptococcus pyogenes. It can lead to multiorgan failure and, ultimately, death. It is important to be aware of TSS, as it can present within any setting. Patients often have non-specific symptoms and their condition can worsen rapidly. TSS postorthopaedic surgery is rare; however, due to the serious nature of this disease, it is important to promptly recognise and diagnose TSS, and to ensure appropriate treatment is started without delay. PMID:26264942

Toxic shock syndrome caused by suture abscess with methicillin-resistant Staphylococcus aureus (MRSA) with late onset after Caesarean section.

PubMed

Komuro, Hiroyasu; Kato, Takaharu; Okada, Shinichiro; Nakatani, Kensuke; Matsumoto, Risa; Nishida, Kazuhiro; Iida, Hiroyuki; Iida, Maki; Tsujimoto, Shiro; Suganuma, Toshiyuki

2017-01-01

Toxic shock syndrome (TSS) is a rare but life-threatening multisystem disease known to develop in the early postoperative period after various surgery. We report a rare case in which a patient who underwent Caesarean section developed TSS caused by methicillin-resistant Staphylococcus aureus (MRSA) on the 39th postoperative day. She was treated with debridement because of the possible diagnosis of necrotizing soft tissue infections. Culture test from the resected specimen was positive for MRSA. She was diagnosed with TSS caused by suture abscess and was treated with intensive care including antimicrobials. After a good postoperative course, she was discharged on the 30th postoperative day. TSS occurring 4 weeks after operation is extremely rare, but late-onset of suture abscess is known to occur. We should becognizant of development with TSS beyond early postoperative period.

Polymyxin-B immobilized column-direct hemoperfusion for adolescent toxic shock syndrome.

PubMed

Nanishi, Etsuro; Hirata, Yuichirou; Lee, Sooyoung; Kaku, Noriyuki; Momii, Kenta; Kubota, Kensuke; Nishio, Hisanori; Maehara, Yoshihiko; Hara, Toshiro

2016-10-01

Toxic shock syndrome (TSS) is a critical illness associated with toxin from Staphylococcus aureus. Despite recent advances in critical care, mortality remains high and additional effective therapy is required. We report an adolescent case of TSS successfully treated with direct hemoperfusion using polymyxin-B immobilized fiber (PMX-DHP). The patient with spina bifida also had ischial pressure ulcer, and developed TSS associated with methicillin-resistant S. aureus. Despite conventional treatment, the patient developed refractory shock, which was immediately improved with PMX-DHP. PMX-DHP has been widely used for the treatment of sepsis to remove circulating endotoxins produced by Gram-negative bacteria, but beneficial effects have also been reported for Gram-positive bacterial infection. To our knowledge, this is the first report on PMX-DHP for TSS in an adolescent patient, and we propose that PMX-DHP could be a new treatment strategy for severe TSS in children as well. © 2016 Japan Pediatric Society.

Food-service establishment wastewater characterization.

PubMed

Lesikar, B J; Garza, O A; Persyn, R A; Kenimer, A L; Anderson, M T

2006-08-01

Food-service establishments that use on-site wastewater treatment systems are experiencing pretreatment system and/or drain field hydraulic and/or organic overloading. This study included characterization of four wastewater parameters (five-day biochemical oxygen demand [BOD5]; total suspended solids [TSS]; food, oil, and grease [FOG]; and flow) from 28 restaurants located in Texas during June, July, and August 2002. The field sampling methodology included taking a grab sample from each restaurant for 6 consecutive days at approximately the same time each day, followed by a 2-week break, and then sampling again for another 6 consecutive days, for a total of 12 samples per restaurant and 336 total observations. The analysis indicates higher organic (BOD5) and hydraulic values for restaurants than those typically found in the literature. The design values for this study for BOD5, TSS, FOG, and flow were 1523, 664, and 197 mg/L, and 96 L/day-seat respectively, which captured over 80% of the data collected.

Influence of scary beliefs about the Tuskegee Syphilis Study on willingness to participate in research.

PubMed

Davis, Jenna L; Green, B Lee; Katz, Ralph V

2012-01-01

To assess whether scary/alarming beliefs about details on the Tuskegee Syphilis Study (TSS) are associated with willingness and/or fear to participate in biomedical research. Scary beliefs about TSS were examined for 565 Black and White adults who had heard of the TSS. Multivariate analyses by race were used to measure association. No association between scary beliefs and willingness or fear to participate in research was found (P > 0.05). These findings provide additional evidence that awareness or detailed knowledge about the TSS does not appear today to be a major factor influencing Blacks' willingness to participate in research.

Modelling the influence of total suspended solids on E. coli removal in river water.

PubMed

Qian, Jueying; Walters, Evelyn; Rutschmann, Peter; Wagner, Michael; Horn, Harald

2016-01-01

Following sewer overflows, fecal indicator bacteria enter surface waters and may experience different lysis or growth processes. A 1D mathematical model was developed to predict total suspended solids (TSS) and Escherichia coli concentrations based on field measurements in a large-scale flume system simulating a combined sewer overflow. The removal mechanisms of natural inactivation, UV inactivation, and sedimentation were modelled. For the sedimentation process, one, two or three particle size classes were incorporated separately into the model. Moreover, the UV sensitivity coefficient α and natural inactivation coefficient kd were both formulated as functions of TSS concentration. It was observed that the E. coli removal was predicted more accurately by incorporating two particle size classes. However, addition of a third particle size class only improved the model slightly. When α and kd were allowed to vary with the TSS concentration, the model was able to predict E. coli fate and transport at different TSS concentrations accurately and flexibly. A sensitivity analysis revealed that the mechanisms of UV and natural inactivation were more influential at low TSS concentrations, whereas the sedimentation process became more important at elevated TSS concentrations.

Ratios of total suspended solids to suspended sediment concentrations by particle size

USGS Publications Warehouse

Selbig, W.R.; Bannerman, R.T.

2011-01-01

Wet-sieving sand-sized particles from a whole storm-water sample before splitting the sample into laboratory-prepared containers can reduce bias and improve the precision of suspended-sediment concentrations (SSC). Wet-sieving, however, may alter concentrations of total suspended solids (TSS) because the analytical method used to determine TSS may not have included the sediment retained on the sieves. Measuring TSS is still commonly used by environmental managers as a regulatory metric for solids in storm water. For this reason, a new method of correlating concentrations of TSS and SSC by particle size was used to develop a series of correction factors for SSC as a means to estimate TSS. In general, differences between TSS and SSC increased with greater particle size and higher sand content. Median correction factors to SSC ranged from 0.29 for particles larger than 500m to 0.85 for particles measuring from 32 to 63m. Great variability was observed in each fraction-a result of varying amounts of organic matter in the samples. Wide variability in organic content could reduce the transferability of the correction factors. ?? 2011 American Society of Civil Engineers.

Evolutionary analysis of a novel zinc ribbon in the N-terminal region of threonine synthase.

PubMed

Kaur, Gurmeet; Subramanian, Srikrishna

2017-10-18

Threonine synthase (TS) catalyzes the terminal reaction in the biosynthetic pathway of threonine and requires pyridoxal phosphate as a cofactor. TSs share a common catalytic domain with other fold type II PALP dependent enzymes. TSs are broadly grouped into two classes based on their sequence, quaternary structure, and enzyme regulation. We report the presence of a novel zinc ribbon domain in the N-terminal region preceding the catalytic core in TS. The zinc ribbon domain is present in TSs belonging to both classes. Our sequence analysis reveals that archaeal TSs possess all zinc chelating residues to bind a metal ion that are lacking in the structurally characterized homologs. Phylogenetic analysis suggests that TSs with an N-terminal zinc ribbon likely represents the ancestral state of the enzyme while TSs without a zinc ribbon must have diverged later in specific lineages. The zinc ribbon and its N- and C-terminal extensions are important for enzyme stability, activity and regulation. It is likely that the zinc ribbon domain is involved in higher order oligomerization or mediating interactions with other biomolecules leading to formation of larger metabolic complexes.

Evaluation of Total Suspended Sediment (TSS) Distribution Using ASTER, ALOS, SPOT-4 Satellite Imagery in 2005-2012

NASA Astrophysics Data System (ADS)

Hariyanto, T.; Krisna, T. C.; Pribadi, C. B.; Kurniawan, A.; Sukojo, B. M.; Taufik, M.

2017-12-01

Lapindo mud thrown to Porong River from September 27, 2006 brought an enormous impact to the environment and surrounding communities. This will exacerbate the damage Porong ecosystems, and pollute the Madura Strait and surrounding areas (Wibisono, 2006). Disposal of sludge in large quantities and continuously to Porong also indicated sedimentation resulted in Porong River, Porong River estuary and along coastal of Surabaya-Pasuruan. This is because the material sediment transport along water flow, and the influence of geographical conditions, and the waves of the sea water. Satellite image data used in this study is the ASTER in 2005-2008, ALOS/AVNIR-2 in 2010, and SPOT-4 years 2009.2011 and 2012. In the satellite image processing, for obtain the value of is used TSS algorithm of Jing Li (2008) for ASTER satellite imagery, algorithms of Hendrawan and Asai (2008) for the ALOS satellite imagery, and algorithm of Budiman (2004) for the SPOT-4 satellite imagery. TSS value of the image processing results then performed validation / test precision using reference data TSS In-Situ to obtain linear correlation (R2). R2 value was obtained is 0.854 in 2009, 0.761 in 2011, and 0712 in 2013. That indicates that the value of TSS in the field is proportional with the TSS value in image and has a very good correlation. The results show the value of TSS in the study area ranged from 25 until more than 150 mg/L and according to the results of the analysis showed an upward trend of TSS values over time. There are several locations that indicated experiencing severe sedimentation impacts such as in Porong River, Porong River Estuary, Alo River Estuary, and the surrounding area of the estuary. According to Government Regulation Number 82 in 2001, the maximum value of TSS in the river or water is must less than 50 mg/L and so the value of TSS in the study area is very improper that if allowed to continue may damage the ecosystem in the area. Results from this study is expected to be referenced in the rehabilitation, development, and utilization of the area in the research area and coastal zone management in the future.

The NTI-tss device for the therapy of bruxism, temporomandibular disorders, and headache – Where do we stand? A qualitative systematic review of the literature

PubMed Central

Stapelmann, Henrike; Türp, Jens C

2008-01-01

Background The NTI-tss device is an anterior bite stop, which, according to the manufacturer, is indicated for the prevention and treatment of bruxism, temporomandibular disorders (TMDs), tension-type headaches, and migraine. The aim of this systematic review was to appraise the currently available evidence regarding the efficacy and safety of the NTI-tss splint. Methods We performed a systematic search in nine electronic databases and in NTI-tss-associated websites (last update: December 31, 2007). The reference lists of all relevant articles were perused. Five levels of scientific quality were distinguished. Reporting quality of articles about randomized controlled trials (RCTs) was evaluated using the Jadad score. To identify adverse events, we searched in the identified publications and in the MAUDE database. Results Nine of 68 relevant publications reported about the results of five different RCTs. Two RCTs concentrated on electromyographic (EMG) investigations in patients with TMDs and concomitant bruxism (Baad-Hansen et al 2007, Jadad score: 4) or with bruxism alone (Kavaklı 2006, Jadad score: 2); in both studies, compared to an occlusal stabilization splint the NTI-tss device showed significant reduction of EMG activity. Two RCTs focused exclusively on TMD patients; in one trial (Magnusson et al 2004, Jadad score: 3), a stabilization appliance led to greater improvement than an NTI-tss device, while in the other study (Jokstad et al 2005, Jadad score: 5) no difference was found. In one RCT (Shankland 2002, Jadad score: 1), patients with tension-type headache or migraine responded more favorably to the NTI-tss splint than to a bleaching tray. NTI-tss-induced complications related predominantly to single teeth or to the occlusion. Conclusion Evidence from RCTs suggests that the NTI-tss device may be successfully used for the management of bruxism and TMDs. However, to avoid potential unwanted effects, it should be chosen only if certain a patient will be compliant with follow-up appointments. The NTI-tss bite splint may be justified when a reduction of jaw closer muscle activity (e.g., jaw clenching or tooth grinding) is desired, or as an emergency device in patients with acute temporomandibular pain and, possibly, restricted jaw opening. PMID:18662411

Differences in Surface Water Quality Draining Four Road Surface Types in the Southern Appalachians

Treesearch

Barton D. Clinton; James M. Vose

2003-01-01

Improved and unimproved roads can be the primary source of stream sediment in forested watersheds. We assessed differences in production of total suspended solids (TSS; ppm) from four road sulfate conditions in a Southern Appalachian watershed: (1) a 2-yr-old paved surface (P), (2) an improved gravel sulfate with controlled drainage and routine maintenance (RG), (3) an...

40 CFR 406.62 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of rice) BOD5 0.42 0.14 TSS 0.24 .08 pH (1) (1) English units...

40 CFR 406.62 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of rice) BOD5 0.42 0.14 TSS 0.24 .08 pH (1) (1) English units...

40 CFR 406.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 0.21 0.07 TSS 0.18 .06 pH (1) (1) English units...

40 CFR 406.42 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.025 0.0083 TSS 0.025 .0083 pH (1) (1) English units...

40 CFR 406.42 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.025 0.0083 TSS 0.025 .0083 pH (1) (1) English units...

40 CFR 406.42 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.025 0.0083 TSS 0.025 .0083 pH (1) (1) English units...

40 CFR 406.42 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Bulgur Wheat Flour... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of wheat) BOD5 0.025 0.0083 TSS 0.025 .0083 pH (1) (1) English units...

40 CFR 406.62 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Parboiled Rice... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of rice) BOD5 0.42 0.14 TSS 0.24 .08 pH (1) (1) English units...

40 CFR 406.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 0.21 0.07 TSS 0.18 .06 pH (1) (1) English units...

40 CFR 406.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Corn Dry Milling... Effluent limitations Maximum for any 1 day Average of daily values for 30 consecutive days shall not exceed— Metric units (kilograms per 1,000 kg of corn) BOD5 0.21 0.07 TSS 0.18 .06 pH (1) (1) English units...

Unique Results and Lessons Learned From the TSS Missions

NASA Technical Reports Server (NTRS)

Stone, Nobie H.

2016-01-01

The Tethered Satellite System (TSS) Space Shuttle missions, TSS-1 in 1993 and TSS-1R in 1996, were the height of space tether technology development in the U.S. Altogether, the investment made by NASA and the Italian Space Agency (ASI) over the thirteen-year period of the TSS Program totaled approximately $400M-exclusive of the two Space Shuttle flights provided by NASA. Since those two pioneering missions, there have been several smaller tether flight experiments, but interest in this promising technology has waned within NASA as well as the DOD agencies. This is curious in view of the unique capabilities of space tether systems and the fact that they have been flight validated in earth orbit and shown to perform better than the preflight dynamic or electrodynamic theoretical predictions. While it is true that the TSS-1 and TSS-1R missions experienced technical difficulties, the causes of these early developmental problems are now known to have been engineering design flaws, material selection, and procedural issues that (1) are unrelated to the basic viability of space tether technology, and (2) can be readily corrected. The purpose of this paper is to review the dynamic and electrodynamic fundamentals of space tethers and the unique capabilities they afford (that are enabling to certain types of space missions); to elucidate the nature, cause, and solution of the early developmental problems; and to provide an update on progress made in development of the technology.

Stochastic evaluation of annual micropollutant loads and their uncertainties in separate storm sewers.

PubMed

Hannouche, Ali; Chebbo, Ghassan; Joannis, Claude; Gasperi, Johnny; Gromaire, Marie-Christine; Moilleron, Régis; Barraud, Sylvie; Ruban, Véronique

2017-12-01

This article describes a stochastic method to calculate the annual pollutant loads and its application over several years at the outlet of three catchments drained by separate storm sewers. A stochastic methodology using Monte Carlo simulations is proposed for assessing annual pollutant load, as well as the associated uncertainties, from a few event sampling campaigns and/or continuous turbidity measurements (representative of the total suspended solids concentration (TSS)). Indeed, in the latter case, the proposed method takes into account the correlation between pollutants and TSS. The developed method was applied to data acquired within the French research project "INOGEV" (innovations for a sustainable management of urban water) at the outlet of three urban catchments drained by separate storm sewers. Ten or so event sampling campaigns for a large range of pollutants (46 pollutants and 2 conventional water quality parameters: TSS and total organic carbon (TOC)) are combined with hundreds of rainfall events for which, at least one among three continuously monitored parameters (rainfall intensity, flow rate, and turbidity) is available. Results obtained for the three catchments show that the annual pollutant loads can be estimated with uncertainties ranging from 10 to 60%, and the added value of turbidity monitoring for lowering the uncertainty is demonstrated. A low inter-annual and inter-site variability of pollutant loads, for many of studied pollutants, is observed with respect to the estimated uncertainties, and can be explained mainly by annual precipitation.

Staphylococcal toxic shock syndrome 2000-2006: epidemiology, clinical features, and molecular characteristics.

PubMed

DeVries, Aaron S; Lesher, Lindsey; Schlievert, Patrick M; Rogers, Tyson; Villaume, Lourdes G; Danila, Richard; Lynfield, Ruth

2011-01-01

Circulating strains of Staphylococcus aureus (SA) have changed in the last 30 years including the emergence of community-associated methicillin-resistant SA (MRSA). A report suggested staphylococcal toxic shock syndrome (TSS) was increasing over 2000-2003. The last population-based assessment of TSS was 1986. Population-based active surveillance for TSS meeting the CDC definition using ICD-9 codes was conducted in the Minneapolis-St. Paul area (population 2,642,056) from 2000-2006. Medical records of potential cases were reviewed for case criteria, antimicrobial susceptibility, risk factors, and outcome. Superantigen PCR testing and PFGE were performed on available isolates from probable and confirmed cases. Of 7,491 hospitalizations that received one of the ICD-9 study codes, 61 TSS cases (33 menstrual, 28 non-menstrual) were identified. The average annual incidence per 100,000 of all, menstrual, and non-menstrual TSS was 0.52 (95% CI, 0.32-0.77), 0.69 (0.39-1.16), and 0.32 (0.12-0.67), respectively. Women 13-24 years had the highest incidence at 1.41 (0.63-2.61). No increase in incidence was observed from 2000-2006. MRSA was isolated in 1 menstrual and 3 non-menstrual cases (7% of TSS cases); 1 isolate was USA400. The superantigen gene tst-1 was identified in 20 (80%) of isolates and was more common in menstrual compared to non-menstrual isolates (89% vs. 50%, p = 0.07). Superantigen genes sea, seb and sec were found more frequently among non-menstrual compared to menstrual isolates [100% vs 25% (p = 0.4), 60% vs 0% (p<0.01), and 25% vs 13% (p = 0.5), respectively]. TSS incidence remained stable across our surveillance period of 2000-2006 and compared to past population-based estimates in the 1980s. MRSA accounted for a small percentage of TSS cases. tst-1 continues to be the superantigen associated with the majority of menstrual cases. The CDC case definition identifies the most severe cases and has been consistently used but likely results in a substantial underestimation of the total TSS disease burden.

Interferon gamma-dependent intestinal pathology contributes to the lethality in bacterial superantigen-induced toxic shock syndrome.

PubMed

Tilahun, Ashenafi Y; Holz, Marah; Wu, Tsung-Teh; David, Chella S; Rajagopalan, Govindarajan

2011-02-03

Toxic shock syndrome (TSS) caused by the superantigen exotoxins of Staphylococcus aureus and Streptococcus pyogenes is characterized by robust T cell activation, profound elevation in systemic levels of multiple cytokines, including interferon-γ (IFN-γ), followed by multiple organ dysfunction and often death. As IFN-γ possesses pro- as well as anti-inflammatory properties, we delineated its role in the pathogenesis of TSS. Antibody-mediated in vivo neutralization of IFN-γ or targeted disruption of IFN-γ gene conferred significant protection from lethal TSS in HLA-DR3 transgenic mice. Following systemic high dose SEB challenge, whereas the HLA-DR3.IFN-γ(+/+) mice became sick and succumbed to TSS, HLA-DR3.IFN-γ(-/-) mice appeared healthy and were significantly protected from SEB-induced lethality. SEB-induced systemic cytokine storm was significantly blunted in HLA-DR3.IFN-γ(-/-) transgenic mice. Serum concentrations of several cytokines (IL-4, IL-10, IL-12p40 and IL-17) and chemokines (KC, rantes, eotaxin and MCP-1) were significantly lower in HLA-DR3.IFN-γ(-/-) transgenic mice. However, SEB-induced T cell expansion in the spleens was unaffected and expansion of SEB-reactive TCR Vβ8(+) CD4(+) and CD8(+) T cells was even more pronounced in HLA-DR3.IFN-γ(-/-) transgenic mice when compared to HLA-DR3.IFN-γ(+/+) mice. A systematic histopathological examination of several vital organs revealed that both HLA-DR3.IFN-γ(+/+) and HLA-DR3.IFN-γ(-/-) transgenic mice displayed comparable severe inflammatory changes in lungs, and liver during TSS. Remarkably, whereas the small intestines from HLA-DR3.IFN-γ(+/+) transgenic mice displayed significant pathological changes during TSS, the architecture of small intestines in HLA-DR3.IFN-γ(-/-) transgenic mice was preserved. In concordance with these histopathological changes, the gut permeability to macromolecules was dramatically increased in HLA-DR3.IFN-γ(+/+) but not HLA-DR3.IFN-γ(-/-) mice during TSS. Overall, IFN-γ seemed to play a lethal role in the immunopathogenesis of TSS by inflicting fatal small bowel pathology. Our study thus identifies the important role for IFN-γ in TSS.

A Modified Hopfield Neural Network Algorithm (MHNNA) Using ALOS Image for Water Quality Mapping

PubMed Central

Kzar, Ahmed Asal; Mat Jafri, Mohd Zubir; Mutter, Kussay N.; Syahreza, Saumi

2015-01-01

Decreasing water pollution is a big problem in coastal waters. Coastal health of ecosystems can be affected by high concentrations of suspended sediment. In this work, a Modified Hopfield Neural Network Algorithm (MHNNA) was used with remote sensing imagery to classify the total suspended solids (TSS) concentrations in the waters of coastal Langkawi Island, Malaysia. The adopted remote sensing image is the Advanced Land Observation Satellite (ALOS) image acquired on 18 January 2010. Our modification allows the Hopfield neural network to convert and classify color satellite images. The samples were collected from the study area simultaneously with the acquiring of satellite imagery. The sample locations were determined using a handheld global positioning system (GPS). The TSS concentration measurements were conducted in a lab and used for validation (real data), classification, and accuracy assessments. Mapping was achieved by using the MHNNA to classify the concentrations according to their reflectance values in band 1, band 2, and band 3. The TSS map was color-coded for visual interpretation. The efficiency of the proposed algorithm was investigated by dividing the validation data into two groups. The first group was used as source samples for supervisor classification via the MHNNA. The second group was used to test the MHNNA efficiency. After mapping, the locations of the second group in the produced classes were detected. Next, the correlation coefficient (R) and root mean square error (RMSE) were calculated between the two groups, according to their corresponding locations in the classes. The MHNNA exhibited a higher R (0.977) and lower RMSE (2.887). In addition, we test the MHNNA with noise, where it proves its accuracy with noisy images over a range of noise levels. All results have been compared with a minimum distance classifier (Min-Dis). Therefore, TSS mapping of polluted water in the coastal Langkawi Island, Malaysia can be performed using the adopted MHNNA with remote sensing techniques (as based on ALOS images). PMID:26729148

Influence of scary beliefs about the Tuskegee Syphilis Study on willingness to participate in research

PubMed Central

Davis, Jenna L.; Green, B. Lee; Katz, Ralph V.

2013-01-01

Objectives To assess whether scary/alarming beliefs about details on the Tuskegee Syphilis Study (TSS) are associated with willingness and/or fear to participate in biomedical research. Methods Scary beliefs about TSS were examined for 565 Black and White adults who had heard of the TSS. Multivariate analyses by race were used to measure association. Results No association between scary beliefs and willingness or fear to participate in research was found (P>0.05). Conclusions These findings provide additional evidence that awareness or detailed knowledge about the TSS does not appear today to be a major factor influencing Blacks’ willingness to participate in research. PMID:22924230

Enhancing dewaterability of waste activated sludge by combined oxidative conditioning process with zero-valent iron and peroxymonosulfate.

PubMed

Zhou, Xu; Jin, Wenbiao; Chen, Hongyi; Chen, Chuan; Han, Songfang; Tu, Renjie; Wei, Wei; Gao, Shu-Hong; Xie, Guo-Jun; Wang, Qilin

2017-11-01

The enhancement of sludge dewaterability is of great importance for facilitating the sludge disposal during the operation of wastewater treatment plants. In this study, a novel oxidative conditioning approach was applied to enhance the dewaterability of waste activated sludge by the combination of zero-valent iron (ZVI) and peroxymonosulfate (PMS). It was found that the dewaterability of sludge was significantly improved after the addition of ZVI (0-4 g/g TSS) (TSS: total suspended solids) and PMS (0-1 g/g TSS). The optimal addition amount of ZVI and PMS was 0.25 g/g TSS and 0.1 g/g TSS, respectively, under which the capillary suction time of the sludge was reduced by approximately 50%. The decomposition of sludge flocs could contribute to the improved sludge dewaterability. Economic analysis demonstrated that the proposed conditioning process with ZVI and PMS was more economical than the ZVI + peroxydisulfate and the traditional Fenton conditioning processes.

Toxic shock syndrome post open reduction and Kirschner wire fixation of a humeral lateral condyle fracture.

PubMed

Chan, Yuen; Selvaratnam, Veenesh; Garg, Neeraj

2015-08-11

Use of Kirschner wires (K-wires) is the most common method of fracture stabilisation in lateral condyle fracture fixation in children. We report a case of toxic shock syndrome (TSS) following an open reduction and internal fixation using K-wires for a humeral lateral condyle fracture in a 5-year-old girl. TSS is a toxin-mediated multisystem illness. It typically presents with shock and it is most often attributed to toxin-producing strains of Staphylococcus aureus and Streptococcus pyogenes. It can lead to multiorgan failure and, ultimately, death. It is important to be aware of TSS, as it can present within any setting. Patients often have non-specific symptoms and their condition can worsen rapidly. TSS postorthopaedic surgery is rare; however, due to the serious nature of this disease, it is important to promptly recognise and diagnose TSS, and to ensure appropriate treatment is started without delay. 2015 BMJ Publishing Group Ltd.

Unusual staphylococcal toxic shock syndrome presenting as a scarlet-like fever.

PubMed

Andrey, D O; Ferry, T; Siegenthaler, N; Fletcher, C; Calmy, A; Lina, G; Emonet, S

2015-11-01

Diagnosis of nonmenstrual staphylococcal toxic shock syndrome (TSS) is often challenging. A female medical colleague had a rare entity, a staphylococcal pharyngitis complicated by TSS. The diagnosis was confirmed by isolation of tst-positive Staphylococcus aureus in throat culture and by identification of a specific Vβ2 expansion pattern of her T lymphocytes. Recent improvements in microbiology can be of great help for the diagnosis of nonmenstrual TSS.

40 CFR 437.43 - Effluent limitations attainable by the application of the best conventional pollutant control...

Code of Federal Regulations, 2010 CFR

2010-07-01

...&G, pH, and TSS are the same as the corresponding limitation specified in § 437.42(b). (c) Combined waste receipts from subparts A and B of this part: Limitations for O&G, pH, and TSS are the same as the... part: Limitations for BOD5, O&G, pH, and TSS are the same as the corresponding limitation specified in...

40 CFR 437.43 - Effluent limitations attainable by the application of the best conventional pollutant control...

Code of Federal Regulations, 2011 CFR

2011-07-01

...&G, pH, and TSS are the same as the corresponding limitation specified in § 437.42(b). (c) Combined waste receipts from subparts A and B of this part: Limitations for O&G, pH, and TSS are the same as the... part: Limitations for BOD5, O&G, pH, and TSS are the same as the corresponding limitation specified in...

Ozonation strategies to reduce sludge production of a seafood industry WWTP.

PubMed

Campos, J L; Otero, L; Franco, A; Mosquera-Corral, A; Roca, E

2009-02-01

In this work, several alternatives related to the application of ozone in different streams of a seafood industry WWTP were evaluated to minimize the production of waste sludge. The WWTP was composed of two coagulation-flocculation units and a biological unit and generated around of 6550 kg/d of sludge. Ozone was applied to sludge coming from flotation units (110 g TSS/L) at doses up to 0.03 g O(3)/g TSS during batch tests, no solids solubilization being observed. Ozone doses ranging from 0.007 to 0.02 g O(3)/g TSS were also applied to the raw wastewater in a bubble column reaching a 6.8% of TSS removal for the highest ozone dose. Finally, the effect of the pre-ozonation (0.05 g O(3)/g TSS) of wastewater coming from the first flotation unit was tested in two activated sludge systems during 70 days. Ozonation caused a reduction of the observed yield coefficient of biomass from 0.14 to 0.07g TSS/g COD(Tremoved) and a slight improvement of COD removal efficiencies. On the basis of the capacity for ozone production available in the industry, a maximum reduction of sludge generated by the WWTP of 7.5% could be expected.

Analysing a cycling grand tour: Can we monitor fatigue with intensity or load ratios?

PubMed

Sanders, Dajo; Heijboer, Mathieu; Hesselink, Matthijs K C; Myers, Tony; Akubat, Ibrahim

2018-06-01

This study evaluated the changes in ratios of different intensity (rating of perceived exertion; RPE, heart rate; HR, power output; PO) and load measures (session-RPE; sRPE, individualized TRIMP; iTRIMP, Training Stress Score™; TSS) in professional cyclists. RPE, PO and HR data was collected from twelve professional cyclists (VO 2max 75 ± 6 ml∙min∙kg -1 ) during a two-week baseline training period and during two cycling Grand Tours. Subjective:objective intensity (RPE:HR, RPE:PO) and load (sRPE:iTRIMP, sRPE:TSS) ratios and external:internal intensity (PO:HR) and load (TSS:iTRIMP) ratios were calculated for every session. Moderate to large increases in the RPE:HR, RPE:PO and sRPE:TSS ratios (d = 0.79-1.79) and small increases in the PO:HR and sRPE:iTRIMP ratio (d = 0.21-0.41) were observed during Grand Tours compared to baseline training data. Differences in the TSS:iTRIMP ratio were trivial to small (d = 0.03-0.27). Small to moderate week-to-week changes (d = 0.21-0.63) in the PO:HR, RPE:PO, RPE:HR, TSS:iTRIMP, sRPE:iTRIMP and sRPE:TSS were observed during the Grand Tour. Concluding, this study shows the value of using ratios of intensity and load measures in monitoring cyclists. Increases in ratios could reflect progressive fatigue that is not readily detected by changes in solitary intensity/load measures.

Impact of pH and Total Soluble Solids on Enzyme Inactivation Kinetics during High Pressure Processing of Mango (Mangifera indica) Pulp.

PubMed

Kaushik, Neelima; Nadella, Tejaswi; Rao, P Srinivasa

2015-11-01

This study was undertaken with an aim to enhance the enzyme inactivation during high pressure processing (HPP) with pH and total soluble solids (TSS) as additional hurdles. Impact of mango pulp pH (3.5, 4.0, 4.5) and TSS (15, 20, 25 °Brix) variations on the inactivation of pectin methylesterase (PME), polyphenol oxidase (PPO), and peroxidase (POD) enzymes were studied during HPP at 400 to 600 MPa pressure (P), 40 to 70 °C temperature (T), and 6- to 20-min pressure-hold time (t). The enzyme inactivation (%) was modeled using second order polynomial equations with a good fit that revealed that all the enzymes were significantly affected by HPP. Response surface and contour models predicted the kinetic behavior of mango pulp enzymes adequately as indicated by the small error between predicted and experimental data. The predicted kinetics indicated that for a fixed P and T, higher pulse pressure effect and increased isobaric inactivation rates were possible at lower levels of pH and TSS. In contrast, at a fixed pH or TSS level, an increase in P or T led to enhanced inactivation rates, irrespective of the type of enzyme. PPO and POD were found to have similar barosensitivity, whereas PME was found to be most resistant to HPP. Furthermore, simultaneous variation in pH and TSS levels of mango pulp resulted in higher enzyme inactivation at lower pH and TSS during HPP, where the effect of pH was found to be predominant than TSS within the experimental domain. Exploration of additional hurdles such as pH, TSS, and temperature for enzyme inactivation during high pressure processing of fruits is useful from industrial point of view, as these parameters play key role in preservation process design. © 2015 Institute of Food Technologists®

Efficacy and safety of iota-carrageenan nasal spray versus placebo in early treatment of the common cold in adults: the ICICC trial.

PubMed

Eccles, R; Winther, B; Johnston, S L; Robinson, P; Trampisch, M; Koelsch, S

2015-10-05

Iota-carrageenan (I-C) is active against respiratory viruses in vitro and was effective as nasal spray in three previous clinical trials. The current trial served to further investigate I-C in patients with early common cold symptoms. This randomized, placebo-controlled, double-blind phase IV trial was conducted in 200 adult patients with self-diagnosed colds of <48 h' duration that were confirmed by baseline cold symptom scores. Patients were to self-administer 0.12 % I-C or placebo spray (NaCl 0.5 %) four times daily for four to ten days and record symptom information for ten days. Common respiratory viruses were quantified by RT-PCR during pretreatment and on Day 3 or 4. The primary endpoint was the mean total symptom score (TSS) of eight cold symptoms on Days 2-4 (TSS2-4). Patients in both treatment groups had similar baseline TSSs (mean TSS: 6.75 for I-C and 6.79 for placebo). Viruses were detected in baseline samples from 53 of 98 I-C patients (54.1 %) and 54 of 97 placebo patients (55.7 %). Mean ± SE for TSS2-4 was 5.78 ± 0.25 for I-C patients and 6.39 ± 0.25 for placebo (p = 0.0895). Exploratory analyses after unblinding (TSS2-4 excluding a patient with aberrantly high symptom scores [TSS2-4, ex 1pt]; mean of TSS over Days 1-4 [TSS1-4]; change in TSS1-4 relative to baseline [TSS1-4, rel]) demonstrated treatment differences in favor of I-C (p = 0.0364, p = 0.0495 and p = 0.0421, respectively). For patients with quantifiable rhinovirus/enterovirus at baseline, there was a trend towards greater reduction of virus load at Day 3 or 4 (p = 0.0958; I-C: 90.2 % reduction in viral load; placebo: 72.0 %). Treatments were well tolerated with no differences in adverse event rates. The primary endpoint did not demonstrate a statistically significant difference between I-C and placebo but showed a trend towards I-C benefit. Exploratory analyses indicated significant reduction of cold symptoms in the I-C group relative to placebo during the first four days when symptoms were most severe, and also substantiated I-C's activity against rhinovirus/enterovirus. NCT01944631 (clinicaltrials.gov).

40 CFR 428.75 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/kkg of raw material) Oil and grease 0.26 0.093 TSS 0.50 0.25 pH (1) (1) English units (lb/1,000 lb of raw material) Oil and grease 0.26 0...— Metric units (kg/kkg of raw material) Lead 0.0017 0.0007 English units (lb/1,000 lb of raw material) Lead...

40 CFR 428.75 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/kkg of raw material) Oil and grease 0.26 0.093 TSS 0.50 0.25 pH (1) (1) English units (lb/1,000 lb of raw material) Oil and grease 0.26 0...— Metric units (kg/kkg of raw material) Lead 0.0017 0.0007 English units (lb/1,000 lb of raw material) Lead...

40 CFR 417.93 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Oleum... 0.09 .03 Surfactants 0.09 .03 Oil and grease 0.21 .07 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.07 0.02 COD 0.27 .09 TSS 0.09 .03 Surfactants 0.09 .03 Oil and grease 0...

40 CFR 417.93 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Oleum... 0.09 .03 Surfactants 0.09 .03 Oil and grease 0.21 .07 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.07 0.02 COD 0.27 .09 TSS 0.09 .03 Surfactants 0.09 .03 Oil and grease 0...

40 CFR 417.113 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY SO3... 0.02 .01 Surfactants 0.20 .10 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.20 0.10 COD 0.90 .45 TSS 0.02 .01 Surfactants 0.20 .10 Oil and grease 0...

40 CFR 417.133 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY... 0.04 .02 Surfactants 0.30 .15 Oil and grease 0.06 .03 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.30 0.15 COD 1.50 .75 TSS 0.04 .02 Surfactants 0.30 .15 Oil and grease 0...

40 CFR 417.123 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Sulfamic... 0.02 .01 Surfactants 0.20 .10 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.20 0.10 COD 0.90 .48 TSS 0.02 .01 Surfactants 0.20 .10 Oil and grease 0...

40 CFR 417.133 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY... 0.04 .02 Surfactants 0.30 .15 Oil and grease 0.06 .03 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.30 0.15 COD 1.50 .75 TSS 0.04 .02 Surfactants 0.30 .15 Oil and grease 0...

40 CFR 417.133 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY... 0.04 .02 Surfactants 0.30 .15 Oil and grease 0.06 .03 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.30 0.15 COD 1.50 .75 TSS 0.04 .02 Surfactants 0.30 .15 Oil and grease 0...

40 CFR 417.113 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY SO3... 0.02 .01 Surfactants 0.20 .10 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.20 0.10 COD 0.90 .45 TSS 0.02 .01 Surfactants 0.20 .10 Oil and grease 0...

40 CFR 417.123 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Sulfamic... 0.02 .01 Surfactants 0.20 .10 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.20 0.10 COD 0.90 .48 TSS 0.02 .01 Surfactants 0.20 .10 Oil and grease 0...

40 CFR 417.123 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Sulfamic... 0.02 .01 Surfactants 0.20 .10 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.20 0.10 COD 0.90 .48 TSS 0.02 .01 Surfactants 0.20 .10 Oil and grease 0...

40 CFR 417.113 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY SO3... 0.02 .01 Surfactants 0.20 .10 Oil and grease 0.04 .02 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.20 0.10 COD 0.90 .45 TSS 0.02 .01 Surfactants 0.20 .10 Oil and grease 0...

40 CFR 417.93 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Oleum... 0.09 .03 Surfactants 0.09 .03 Oil and grease 0.21 .07 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.07 0.02 COD 0.27 .09 TSS 0.09 .03 Surfactants 0.09 .03 Oil and grease 0...

Non-lethal Clostridium sordellii bacteraemia in an immunocompromised patient with pleomorphic sarcoma.

PubMed

Bonnecaze, Alex K; Stephens, Sarah Ellen Elza; Miller, Peter John

2016-08-03

Clostridium sordellii is a spore-forming anaerobic Gram-positive rod that has rarely been reported to cause disease in humans. Resultant mortality from infection is estimated at nearly 70% and is most often correlated with gynaecological procedures, intravenous drug abuse or trauma. C. sordellii infection often presents similarly to toxic shock syndrome (TSS); notable features of infection include refractory hypotension, haemoconcentration and marked leucocytosis. Although clinically similar to TSS, a notable difference is C. sordellii infections rarely involve fever. The organism's major toxins include haemorrhagic (TcsH) and lethal factor (TcsL), which function to disrupt cytoskeletal integrity. Current literature suggests treating C. sordelli infection with a broad-spectrum penicillin, metronidazole and clindamycin. We present a case of C. sordellii bacteraemia and septic shock in an immunocompromised patient who was recently diagnosed with pleomorphic gluteal sarcoma. Despite presenting in critical condition, the patient improved after aggressive hemodynamic resuscitation, source control and intravenous antibiotic therapy. 2016 BMJ Publishing Group Ltd.

Spatial and temporal characterizations of water quality in Kuwait Bay.

PubMed

Al-Mutairi, N; Abahussain, A; El-Battay, A

2014-06-15

The spatial and temporal patterns of water quality in Kuwait Bay have been investigated using data from six stations between 2009 and 2011. The results showed that most of water quality parameters such as phosphorus (PO4), nitrate (NO3), dissolved oxygen (DO), and Total Suspended Solids (TSS) fluctuated over time and space. Based on Water Quality Index (WQI) data, six stations were significantly clustered into two main classes using cluster analysis, one group located in western side of the Bay, and other in eastern side. Three principal components are responsible for water quality variations in the Bay. The first component included DO and pH. The second included PO4, TSS and NO3, and the last component contained seawater temperature and turbidity. The spatial and temporal patterns of water quality in Kuwait Bay are mainly controlled by seasonal variations and discharges from point sources of pollution along Kuwait Bay's coast as well as from Shatt Al-Arab River. Copyright © 2014 Elsevier Ltd. All rights reserved.

Investigation on the effectiveness of pretreatment in stormwater management technologies.

PubMed

Maniquiz-Redillas, Marla C; Geronimo, Franz Kevin F; Kim, Lee-Hyung

2014-09-01

The effectiveness of presettling basins as component of stormwater best management practice (BMP) technologies was investigated. Storm event monitoring and sediment collection were conducted from May 2009 to November 2012 on the presettling basins of the three BMP technologies designed to capture and treat stormwater runoff from highly impervious roads and parking lots. Data on captured runoff and sediment, total suspended solids (TSS) loadings, rainfall and runoff rate, sediment accumulation rate, as well as particle distribution and pollutant concentrations of sediment were gathered and analyzed along with the physical design characteristics of the presettling basins such as surface area and storage volume. Regression models were generated to determine significant relationships between design parameters. Results revealed that the storage volume ratio (ratio of storage volume of presettling basin to BMP) was an important parameter in designing the presettling basin of the BMP. For practicality, optimizing the design of the presettling basin means that the storage volume ratio should be determined based on the desired captured amount of runoff and sediment from runoff to limit the frequency of maintenance caused by the accumulation of sediment. It was recommended that pretreatment of runoff should be employed when the site in which the BMP is to be sited has high TSS loading and runoff rate, and is subjected to high intensity rainfall. Copyright © 2014. Published by Elsevier B.V.

Aspects of design, structure, performance and operation of reed beds--eight years' experience in northeastern New South Wales, Australia.

PubMed

Davison, L; Headley, T; Pratt, K

2005-01-01

Reed beds (horizontal subsurface flow constructed wetlands) have been employed as secondary treatment devices in on-site and decentralised wastewater management systems in the northeast of the Australian state of New South Wales (NSW) for over a decade. This paper summarises some of the practical and research findings that have come to light in that time. Experience with various aspects of reed bed structure is discussed. A study of the evaporative performance of four small beds planted with Phragmites australis yielded an annual crop factor of 2.6. A total of 28 studies on reed beds treating a variety of commonly encountered wastewater streams yielded the following mean pollutant removal efficiencies: total suspended solids (TSS) 83%, biochemical oxygen demand (BOD) 81%, total nitrogen (TN) 57%, total phosphorus (TP) 35% and faecal coliforms (FC) 1.9 logs. The reed bed is becoming the preferred on-site technology for removing TN and BOD and polishing TSS from primary settled domestic wastewater. Sizing beds for a residence time of approximately five days has become standard practice. A study of six reed beds found six different species of earthworm present, mainly Perionyx excavatus (Indian Blue). A mesocosm experiment subsequently showed that the worms were translocating clogging material from the substrate interstices to the surface of the bed thereby indicating a possible method for prolonging reed bed life.

Dissolved organic C and N pools in soils amended with composted and thermally-dried sludge as affected by soil tillage systems and sampling depth

NASA Astrophysics Data System (ADS)

García-Gil, Juan Carlos; Soler-Rovira, Pedro Angel; García López de Sa, Esther; Polo, Alfredo

2013-04-01

Soil tillage practices exert a significant influence on the dynamic of soluble organic C and N pools, affecting nutrient cycling in agricultural systems by enhancing its mineralization through microbial activities or stabilization in soil microaggregates, which contribute to mitigate greenhouse gases emissions. The objective of the present research was to determine the influence of three different soil management systems (moldboard plowing, chisel and no-tillage) and the application of composted sludge (CS) and thermally-dried sewage sludge (TSS) obtained from wastewater treatment processes on dissolved organic C (water-soluble organic C -WSOC-, carbohydrates, phenolic compounds) and soluble N (total-N, NH4+, NO3-) pools in a long-term field experiment (27 years) conducted on a sandy-loam soil at the experimental station "La Higueruela" (40° 03'N, 4° 24'W) under semi-arid conditions. Both organic amendments were applied at a rate of 30 tonnes per hectare prior to tillage practices. Unamended soils were used as control for each tillage system. Soil sampling was performed two months after tillage practices at the following depths for each treatment: 0-10 cm, 10-20 cm and 20-30 cm. Results obtained for unamended soils showed that no-tillage management increased total-N, NH4+ and NO3- contents at the 0-10 cm depth samples, meanwhile WSC and carbohydrates contents were larger at 20-30 cm depth samples in both moldboard and no-tillage plots. CS and TSS-amended soils presented a general increase in soluble C and N compounds, being significantly higher in TSS-amended soils, as TSS contains a great amount of labile organic C and N substrates due to the lack of stabilization treatment. TSS-amended soils under no-tillage and chisel plowing showed larger N, NH4+ and NO3- content at the 0-10 cm samples, meanwhile moldboard management exhibited larger NH4+ and NO3- content at 10-20 and 20-30 cm samples, possibly due to the incorporation of TSS at deeper depths (20-40 cm). CS and TSS-amended soils in no-tillage system showed the largest content of organic C pools at 0-10 cm depth samples due to less soil disturbance and the input of organic substrates with CS and TSS on soil surface. CS and TSS-amended soils under chisel plowing exhibited similar contents of soluble organic C pools at 10-20 and 20-30 cm depth samples and only TSS-amended soils increased significantly WSOC content at 0-10 cm samples. Similarly, contents of WSOC and carbohydrates in moldboard plowing were distributed more uniformly throughout the soil profile due to the turnover of soil and CS and TSS amendments into the plow layer. Acknowledgements: this research was supported by the Spanish CICYT, Project no. CTM2011-25557.

Interferon Gamma-Dependent Intestinal Pathology Contributes to the Lethality in Bacterial Superantigen-Induced Toxic Shock Syndrome

PubMed Central

Tilahun, Ashenafi Y.; Holz, Marah; Wu, Tsung-Teh; David, Chella S.; Rajagopalan, Govindarajan

2011-01-01

Toxic shock syndrome (TSS) caused by the superantigen exotoxins of Staphylococcus aureus and Streptococcus pyogenes is characterized by robust T cell activation, profound elevation in systemic levels of multiple cytokines, including interferon-γ (IFN-γ), followed by multiple organ dysfunction and often death. As IFN-γ possesses pro- as well as anti-inflammatory properties, we delineated its role in the pathogenesis of TSS. Antibody-mediated in vivo neutralization of IFN-γ or targeted disruption of IFN-γ gene conferred significant protection from lethal TSS in HLA-DR3 transgenic mice. Following systemic high dose SEB challenge, whereas the HLA-DR3.IFN-γ+/+ mice became sick and succumbed to TSS, HLA-DR3.IFN-γ−/− mice appeared healthy and were significantly protected from SEB-induced lethality. SEB-induced systemic cytokine storm was significantly blunted in HLA-DR3.IFN-γ−/− transgenic mice. Serum concentrations of several cytokines (IL-4, IL-10, IL-12p40 and IL-17) and chemokines (KC, rantes, eotaxin and MCP-1) were significantly lower in HLA-DR3.IFN-γ−/− transgenic mice. However, SEB-induced T cell expansion in the spleens was unaffected and expansion of SEB-reactive TCR Vβ8+ CD4+ and CD8+ T cells was even more pronounced in HLA-DR3.IFN-γ−/− transgenic mice when compared to HLA-DR3.IFN-γ+/+ mice. A systematic histopathological examination of several vital organs revealed that both HLA-DR3.IFN-γ+/+ and HLA-DR3.IFN-γ−/− transgenic mice displayed comparable severe inflammatory changes in lungs, and liver during TSS. Remarkably, whereas the small intestines from HLA-DR3.IFN-γ+/+ transgenic mice displayed significant pathological changes during TSS, the architecture of small intestines in HLA-DR3.IFN-γ−/− transgenic mice was preserved. In concordance with these histopathological changes, the gut permeability to macromolecules was dramatically increased in HLA-DR3.IFN-γ+/+ but not HLA-DR3.IFN-γ−/− mice during TSS. Overall, IFN-γ seemed to play a lethal role in the immunopathogenesis of TSS by inflicting fatal small bowel pathology. Our study thus identifies the important role for IFN-γ in TSS. PMID:21304813

Staphylococcal Toxic Shock Syndrome 2000–2006: Epidemiology, Clinical Features, and Molecular Characteristics

PubMed Central

DeVries, Aaron S.; Lesher, Lindsey; Schlievert, Patrick M.; Rogers, Tyson; Villaume, Lourdes G.; Danila, Richard; Lynfield, Ruth

2011-01-01

Introduction Circulating strains of Staphylococcus aureus (SA) have changed in the last 30 years including the emergence of community-associated methicillin-resistant SA (MRSA). A report suggested staphylococcal toxic shock syndrome (TSS) was increasing over 2000–2003. The last population-based assessment of TSS was 1986. Methods Population-based active surveillance for TSS meeting the CDC definition using ICD-9 codes was conducted in the Minneapolis-St. Paul area (population 2,642,056) from 2000–2006. Medical records of potential cases were reviewed for case criteria, antimicrobial susceptibility, risk factors, and outcome. Superantigen PCR testing and PFGE were performed on available isolates from probable and confirmed cases. Results Of 7,491 hospitalizations that received one of the ICD-9 study codes, 61 TSS cases (33 menstrual, 28 non-menstrual) were identified. The average annual incidence per 100,000 of all, menstrual, and non-menstrual TSS was 0.52 (95% CI, 0.32–0.77), 0.69 (0.39–1.16), and 0.32 (0.12–0.67), respectively. Women 13–24 years had the highest incidence at 1.41 (0.63–2.61). No increase in incidence was observed from 2000–2006. MRSA was isolated in 1 menstrual and 3 non-menstrual cases (7% of TSS cases); 1 isolate was USA400. The superantigen gene tst-1 was identified in 20 (80%) of isolates and was more common in menstrual compared to non-menstrual isolates (89% vs. 50%, p = 0.07). Superantigen genes sea, seb and sec were found more frequently among non-menstrual compared to menstrual isolates [100% vs 25% (p = 0.4), 60% vs 0% (p<0.01), and 25% vs 13% (p = 0.5), respectively]. Discussion TSS incidence remained stable across our surveillance period of 2000–2006 and compared to past population-based estimates in the 1980s. MRSA accounted for a small percentage of TSS cases. tst-1 continues to be the superantigen associated with the majority of menstrual cases. The CDC case definition identifies the most severe cases and has been consistently used but likely results in a substantial underestimation of the total TSS disease burden. PMID:21860665

Role of testis sparing surgery in the conservative management of small testicular masses: oncological and functional perspectives.

PubMed

Borghesi, M; Brunocilla, E; Schiavina, R; Gentile, G; Dababneh, H; Della Mora, L; del Prete, C; Franceschelli, A; Colombo, F; Martorana, G

2015-01-01

Radical orchiectomy (RO) is still considered the standard of care for malignant germ cell tumours, which represent the vast majority of the palpable testicular masses. In those patients diagnosed with small testicular masses (STMs), testis-sparing surgery (TSS) could be an alternative treatment to RO. The aim of this updated review is to evaluate the current indications for TSS, and discuss the oncological and functional results of patients who had undergone organ-sparing surgery for STMs. A non-systematic review of the Literature using the Medline database has been performed, including a free-text protocol using the terms "testis-sparing surgery", "testicular sparing surgery", "partial orchiectomy", "testis tumour", "sex cord tumour", and "testis function". Other significant studies cited in the reference lists of the selected papers were also evaluated. No randomized controlled trials comparing TSS with radical orchiectomy have been reported yet. In those patients with normal contra-lateral testis, the use of TSS is still controversial. In selected cases of gonadal masses < 2 cm, TSS seems to be a safe and feasible treatment option. Frozen section examination allows us to discriminate between benign and malignant neoplasms during TSS. Intermediate and long-term follow-up results showed no significant risk of local and distant recurrences in the main series reported in the literature. TSS is an effective treatment for STMs in selected patients, limiting the unnecessary surgical over-treatments, without compromising the oncological and functional outcomes. Further studies are needed in order to confirm the oncological safety. Copyright © 2013 AEU. Publicado por Elsevier España, S.L.U. All rights reserved.

Systemic Inflammatory Response Elicited by Superantigen Destabilizes T Regulatory Cells Rendering them Ineffective during Toxic Shock Syndrome 1

PubMed Central

Tilahun, Ashenafi Y.; Chowdhary, Vaidehi R.; David, Chella S.; Rajagopalan, Govindarajan

2014-01-01

Life-threatening infections caused by Staphylococcus aureus, particularly the community-acquired methicillin-resistant strains of S. aureus (CA-MRSA), continue to pose serious problems. Greater virulence and increased pathogenicity of certain S. aureus strains are attributed to higher prevalence of exotoxins. Of these exotoxins, the superantigens (SAg) are likely most pathogenic because of their ability to rapidly and robustly activate the T cells even in extremely small quantities. Therefore, countering SAg-mediated T cell activation using T regulatory cells (Tregs) might be beneficial in diseases such as toxic shock syndrome (TSS). As the normal numbers of endogenous Tregs in a typical host are insufficient, we hypothesized that increasing the Treg numbers by administration of IL2-anti-IL2 antibody complexes (IL2C) or by adoptive transfer of ex vivo expanded Tregs might be more effective in countering SAg-mediated immune activation. HLA-DR3 transgenic mice that closely recapitulate human TSS, were treated with IL2C to increase endogenous Tregs or received ex vivo expanded Tregs. Subsequently, they were challenged with SAg to induce TSS. Analyses of various parameters reflective of TSS (serum cytokine/chemokine levels, multiple organ pathology and SAg-induced peripheral T cell expansion) indicated that increasing the Tregs failed to mitigate TSS. On the contrary, serum IFN-γ levels were increased in IL2C treated mice. Exploration into the reasons behind the lack of protective effect of Tregs revealed IL-17 and IFN-γ-dependent loss of Tregs during TSS. In addition, significant upregulation of GITR on conventional T cells during TSS could render them resistant to Treg mediated suppression, contributing to failure of Treg-mediated immune regulation. PMID:25092888

Long-term remission and recurrence rates after first and second transsphenoidal surgery for Cushing's disease: care reality in the Munich Metropolitan Region.

PubMed

Dimopoulou, C; Schopohl, J; Rachinger, W; Buchfelder, M; Honegger, J; Reincke, M; Stalla, G K

2014-02-01

Transsphenoidal surgery (TSS) presents the treatment of choice for Cushing's disease (CD). Remission and recurrence rates vary dependent on tumor size, extension, adenoma visibility on magnetic resonance imaging, and neurosurgical expertise. Other than published from single-surgeon neurosurgical series so far, we have aimed to describe long-term remission and recurrence rates of CD in a series incorporating different neurosurgeons, trying to reflect care reality in the Munich Metropolitan Region, which is accommodated by three tertiary university and multiple, smaller neurosurgical centers. We conducted a retrospective analysis of 120 patients who underwent first and 36 patients who underwent second TSS as treatment for CD between 1990 and 2012. Patients were divided into three groups according to remission status. Potential risk factors for recurrence, pituitary function, and strategy in persistent disease were assessed. THREE OUTCOME GROUPS WERE IDENTIFIED ACCORDING TO REMISSION STATUS AFTER FIRST TSS (MEAN FOLLOW-UP 79 MONTHS): remission, 71% (85/120), disease persistence, 29% (35/120), and disease recurrence, 34% (29/85) (mean time to recurrence 54 months). After second TSS (n=36, mean follow-up 62 months), we documented remission in 42% (15/36), disease persistence in 58% (21/36), and disease recurrence in 40% (6/15) (mean time to recurrence 42 months). Postoperative hypocortisolism after first, though not after second, TSS was associated with a lower risk of suffering disease recurrence (risk=0.72; 95% CI 0.60-0.88; exact significance (two-sided) P=0.035). Our study shows higher recurrence rates of CD after first TSS than previously reported. Second TSS leads an additional 8% of the patients to long-term CD remission.

Feeding strategies during dry summer for lactating desert goats in a rainfed area under tropical conditions.

PubMed

Ahmed, M M.M.; El Hag, F M.; Wahab, F S.; Salih, S F.

2001-02-01

Feeding strategies aimed at improving the nutritive value of crop residues through chemical treatment or utilizing other available feed resources as small ruminant feeds were carried out during the hot dry summer season when rangeland pasture deteriorates drastically. The study utilized small-scale farmer animals and crop residues chemically treated as animal feeds. Other feeds were groundnut haulms (GNH) and tree pods (TP) of Faidherbia albida. A total of 30 adult lactating goats with their kids (30) were allocated in completely randomized design to the following treatments: untreated sorghum straw (USS, control), sorghum straw treated with 4.7% urea solution (TSS), TSS+treepods (TSS+TP), USS+groundnuthaulms (USS+GNH) and TSS+GNH. Parameters investigated were: changes in body weight (BW) of the does and their kids as well as milk composition of the does. The results showed that dry matter intake (DMI) was reduced (P<0.05) by urea treatment, but it was increased (P<0.05) by supplementation. In vitro organic matter digestibility (IVOMD) was improved (P<0.05) by urea treatment and supplementation with either GNH or TP. BW of does decreased (P<0.05) in the TSS group, while a significant increase was obtained in the TSS+GNH group. BW of the kids belonging to the does given USS+GNH or TSS+TP was higher (P<0.05) than BW of the kids that belonged to the does fed other rations. However, no losses in BW were detected. It could be concluded that the combination of urea treatment and supplementation with either GNH or TP improved DMI, BW, digestibility and milk yield.

Ozonation of return activated sludge for disintegration and solubilisation with synthesized titanium oxide as catalyst

NASA Astrophysics Data System (ADS)

Sarif, S. F. Z. Mohd; Alias, S. S.; Ridwan, F. Muhammad; Salim, K. S. Ku; Abidin, C. Z. A.; Ali, U. F. Md.

2018-03-01

Ozonation of activated sludge in the present of titanium dioxide (TiO2) as catalyst to enhance the production of hydroxyl radical was evaluated in comparison to the sole ozonation process. In this process, the catalytic ozontion showed improvement in increasing ozone consumption and improving activated sludge disintegration and solubilisation. The reduction of total suspended solid (TSS), volatile suspended solid (VSS) and soluble chemical oxygen demand (SCOD) solubilisation was better in the catalytic ozonation system. Initial pH 7 of activated sludge was found best to disintegrate and solubilise the sludge flocs. However upon additional of sodium hydroxide (NaOH) in pH adjustment enhanced the solubilisation of organic matter from the flocs and cells, making the initial pH 9 is the best condition for activated sludge solubilisation. Yet the initial pH 7 of activated sludge supernatant was the best condition to achieve SCOD solubilisation due to sludge floc disintegration, when it had stronger correlation between TSS reduction and SCOD solubilisation (R2=0.961). Lower amount of catalyst of 100 mgTiO2/gTSS was found to disintegrate and solubilise the activated sludge better with 30.4% TSS reduction and 25.2% SCOD solubilisation efficiency, compared to 200 mgTiO2/gTSS with 21.9% and 17.1% TSS reduction and SCOD solubilisation, respectively.

Rotating belt sieves for primary treatment, chemically enhanced primary treatment and secondary solids separation.

PubMed

Rusten, B; Rathnaweera, S S; Rismyhr, E; Sahu, A K; Ntiako, J

2017-06-01

Fine mesh rotating belt sieves (RBS) offer a very compact solution for removal of particles from wastewater. This paper shows examples from pilot-scale testing of primary treatment, chemically enhanced primary treatment (CEPT) and secondary solids separation of biofilm solids from moving bed biofilm reactors (MBBRs). Primary treatment using a 350 microns belt showed more than 40% removal of total suspended solids (TSS) and 30% removal of chemical oxygen demand (COD) at sieve rates as high as 160 m³/m²-h. Maximum sieve rate tested was 288 m³/m²-h and maximum particle load was 80 kg TSS/m²-h. When the filter mat on the belt increased from 10 to 55 g TSS/m², the removal efficiency for TSS increased from about 35 to 60%. CEPT is a simple and effective way of increasing the removal efficiency of RBS. Adding about 1 mg/L of cationic polymer and about 2 min of flocculation time, the removal of TSS typically increased from 40-50% without polymer to 60-70% with polymer. Using coagulation and flocculation ahead of the RBS, separation of biofilm solids was successful. Removal efficiencies of 90% TSS, 83% total P and 84% total COD were achieved with a 90 microns belt at a sieve rate of 41 m³/m²-h.

Hybrid zero valent iron (ZVI)/H2O2 oxidation process for landfill leachate treatment with novel nanosize metallic calcium/iron composite.

PubMed

Lee, Son Dong; Mallampati, Srinivasa Reddy; Lee, Byoung Ho

2017-04-01

A novel nanosize metallic calcium/iron dispersed reagent was synthesized and tested as coagulant/catalyst in a hybrid zero valent iron (ZVI)/H 2 O 2 oxidation process to treat leachate. Two different types of leachates, one from municipal solid waste (MSW) tipping hall (MSWIL) and second from an MSW landfill site (MSWLL), were collected and characterized. The morphology, elemental composition, and mineral phases of the nano-Ca/CaO and nano-Fe/Ca/CaO were characterized by scanning electron microscopy-electron dispersive spectroscopy (SEM-EDS) and x-ray powder diffraction (XRD) analysis. The coagulation process with 2.5 g L -1 nano-Ca/CaO attained 64.0, 56.0, and 20.7% removal of color, chemical oxygen demand (COD), and total suspended solids (TSS) in MSWLL. With only 1.0 g L -1 of nano-Fe/Ca/CaO, relatively high color, COD and TSS removal was achieved in MSWLL at 67.5, 60.2, and 37.7%, respectively. The heavy metal removal efficiency reached 91-99% after treatment with nano-Fe/Ca/CaO in both leachate samples. The coupling process, using 1.0 g L -1 of nano-Fe/Ca/CaO and 20 mM H 2 O 2 doses, achieved enhancement removal of color, COD, and TSS, up to 95%, 96%, and 66%, respectively, without initial pH control. After this treatment, the color, COD, TSS, and heavy metals were significantly decreased, fitting the Korean discharge regulation limit. A hybrid coupled zero valent iron (ZVI)/H 2 O 2 oxidation process with novel nanosized metallic calcium/iron dispersed reagent proved to be a suitable treatment for dealing with leachate samples. Conventional treatments (biological or physicochemical) are not sufficient anymore to reach the level of purification needed to fully reduce the negative impact of landfill leachates on the environment. This implies that new treatment alternatives species must be proposed. A coupled zero valent iron (ZVI)/H 2 O 2 oxidation process proved to be a suitable treatment for dealing with leachate samples. Coagulation with nFe/Ca/CaO allows 91-99% of heavy metals removal. The coupled coagulation-oxidation process by nFe/Ca/CaO reveals excellent ability to treat leachate. After coupled treatment the color, COD, and TSS were also much lower than the discharge regulation limit.

Lesson of the month 2: toxic shock syndrome.

PubMed

Shalaby, Tamer; Anandappa, Samantha; Pocock, Nicholas John; Keough, Alexander; Turner, Angus

2014-06-01

Toxic shock syndrome (TSS) represents a fascinating example of immune activation caused by infection resulting in a dramatic and challenging clinical syndrome. TSS is commonly associated with tampon use and still causes significant morbidity and mortality in young healthy women. A misconception is that TSS presents with a skin rash and only occurs in women and children; however, it can occur in males and can present without skin changes. TSS presents initially as a febrile illness and within a few hours can progress to severe hypotension and multiple organ failure (MOF). Staphylococcus aureus and group A beta haemolytic streptococcus (GABHS) can secrete toxins from a small or hidden focus of infection and hence blood culture and sensitivity (C+S) tests can be negative, thereby making diagnosing this condition challenging. Clindamycin is superior to penicillin in the treatment of this condition and significantly decreases the mortality rate in TSS. However, there is also an important role for intravenous immunoglobulins (IVIG). Early intensive care unit (ICU) as well as surgical team involvement (in selected cases) is required to avoid mortality which may approach 70%. © 2014 Royal College of Physicians.

Characterizing potential water quality impacts from soils treated with dust suppressants.

PubMed

Beighley, R Edward; He, Yiping; Valdes, Julio R

2009-01-01

Two separate laboratory experiment series, surface runoff and steady-state seepage, were performed to determine if dust suppressant products can be applied to soils with an expected minimal to no negative impact on water quality. The experiments were designed to mimic arid field conditions and used two soils (clayey and sandy) and six different dust suppressants. The two experiments consisted of: (i) simulated rainfall (intensities of 18, 33, or 61 mm h(-1)) and associated runoff from soil trays at a surface slope of 33%; and (ii) steady-state, constant head seepage through soil columns. Both experiment series involved two product application scenarios and three application ages (i.e., to account for degradation effects) for a total of 126 surface runoff and 80 column experiments. One composite effluent sample was collected from each experiment and analyzed for pH, electrical conductivity, total suspended solids (TSS), total dissolved solids, dissolved oxygen, total organic carbon, nitrate, nitrite, and phosphate. Paired t tests at 1 and 5% levels of significance and project specific data quality objectives are used to compare water quality parameters from treated and untreated soils. Overall, the results from this laboratory scale study suggest that the studied dust suppressants have minimal potential for adverse impacts to selected water quality parameters. The primary impacts were increased TSS for two synthetic products from the surface runoff experiments on both soils. The increase in TSS was not expected based on previous studies and may be attributed to this study's focus on simulating real-world soil agitation/movement at an active construction site subjected to rough grading.

Klang River water quality modelling using music

NASA Astrophysics Data System (ADS)

Zahari, Nazirul Mubin; Zawawi, Mohd Hafiz; Muda, Zakaria Che; Sidek, Lariyah Mohd; Fauzi, Nurfazila Mohd; Othman, Mohd Edzham Fareez; Ahmad, Zulkepply

2017-09-01

Water is an essential resource that sustains life on earth; changes in the natural quality and distribution of water have ecological impacts that can sometimes be devastating. Recently, Malaysia is facing many environmental issues regarding water pollution. The main causes of river pollution are rapid urbanization, arising from the development of residential, commercial, industrial sites, infrastructural facilities and others. The purpose of the study was to predict the water quality of the Connaught Bridge Power Station (CBPS), Klang River. Besides that, affects to the low tide and high tide and. to forecast the pollutant concentrations of the Biochemical Oxygen Demand (BOD) and Total Suspended Solid (TSS) for existing land use of the catchment area through water quality modeling (by using the MUSIC software). Besides that, to identifying an integrated urban stormwater treatment system (Best Management Practice or BMPs) to achieve optimal performance in improving the water quality of the catchment using the MUSIC software in catchment areas having tropical climates. Result from MUSIC Model such as BOD5 at station 1 can be reduce the concentration from Class IV to become Class III. Whereas, for TSS concentration from Class III to become Class II at the station 1. The model predicted a mean TSS reduction of 0.17%, TP reduction of 0.14%, TN reduction of 0.48% and BOD5 reduction of 0.31% for Station 1 Thus, from the result after purposed BMPs the water quality is safe to use because basically water quality monitoring is important due to threat such as activities are harmful to aquatic organisms and public health.

Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design.

PubMed

Smith, Justin D; Suresh, Sundari; Schlecht, Ulrich; Wu, Manhong; Wagih, Omar; Peltz, Gary; Davis, Ronald W; Steinmetz, Lars M; Parts, Leopold; St Onge, Robert P

2016-03-08

Genome-scale CRISPR interference (CRISPRi) has been used in human cell lines; however, the features of effective guide RNAs (gRNAs) in different organisms have not been well characterized. Here, we define rules that determine gRNA effectiveness for transcriptional repression in Saccharomyces cerevisiae. We create an inducible single plasmid CRISPRi system for gene repression in yeast, and use it to analyze fitness effects of gRNAs under 18 small molecule treatments. Our approach correctly identifies previously described chemical-genetic interactions, as well as a new mechanism of suppressing fluconazole toxicity by repression of the ERG25 gene. Assessment of multiple target loci across treatments using gRNA libraries allows us to determine generalizable features associated with gRNA efficacy. Guides that target regions with low nucleosome occupancy and high chromatin accessibility are clearly more effective. We also find that the best region to target gRNAs is between the transcription start site (TSS) and 200 bp upstream of the TSS. Finally, unlike nuclease-proficient Cas9 in human cells, the specificity of truncated gRNAs (18 nt of complementarity to the target) is not clearly superior to full-length gRNAs (20 nt of complementarity), as truncated gRNAs are generally less potent against both mismatched and perfectly matched targets. Our results establish a powerful functional and chemical genomics screening method and provide guidelines for designing effective gRNAs, which consider chromatin state and position relative to the target gene TSS. These findings will enable effective library design and genome-wide programmable gene repression in many genetic backgrounds.

Comparative transcriptomics of two environmentally relevant cyanobacteria reveals unexpected transcriptome diversity

PubMed Central

Voigt, Karsten; Sharma, Cynthia M; Mitschke, Jan; Joke Lambrecht, S; Voß, Björn; Hess, Wolfgang R; Steglich, Claudia

2014-01-01

Prochlorococcus is a genus of abundant and ecologically important marine cyanobacteria. Here, we present a comprehensive comparison of the structure and composition of the transcriptomes of two Prochlorococcus strains, which, despite their similarities, have adapted their gene pool to specific environmental constraints. We present genome-wide maps of transcriptional start sites (TSS) for both organisms, which are representatives of the two most diverse clades within the two major ecotypes adapted to high- and low-light conditions, respectively. Our data suggest antisense transcription for three-quarters of all genes, which is substantially more than that observed in other bacteria. We discovered hundreds of TSS within genes, most notably within 16 of the 29 prochlorosin genes, in strain MIT9313. A direct comparison revealed very little conservation in the location of TSS and the nature of non-coding transcripts between both strains. We detected extremely short 5′ untranslated regions with a median length of only 27 and 29 nt for MED4 and MIT9313, respectively, and for 8% of all protein-coding genes the median distance to the start codon is only 10 nt or even shorter. These findings and the absence of an obvious Shine–Dalgarno motif suggest that leaderless translation and ribosomal protein S1-dependent translation constitute alternative mechanisms for translation initiation in Prochlorococcus. We conclude that genome-wide antisense transcription is a major component of the transcriptional output from these relatively small genomes and that a hitherto unrecognized high degree of complexity and variability of gene expression exists in their transcriptional architecture. PMID:24739626

Neutralization of Staphylococcal Enterotoxin B by an Aptamer Antagonist

PubMed Central

Wang, Kaiyu; Gan, Longjie; Jiang, Li; Zhang, Xianhui; Yang, Xiangyue; Chen, Min

2015-01-01

Staphylococcal enterotoxin B (SEB) is a major virulence factor for staphylococcal toxic shock syndrome (TSS). SEB activates a large subset of the T lymphocytic population, releasing proinflammatory cytokines. Blocking SEB-initiated toxicity may be an effective strategy for treating TSS. Using a process known as systematic evolution of ligands by exponential enrichment (SELEX), we identified an aptamer that can antagonize SEB with nanomolar binding affinity (Kd = 64 nM). The aptamer antagonist effectively inhibits SEB-mediated proliferation and cytokine secretion in human peripheral blood mononuclear cells. Moreover, a PEGylated aptamer antagonist significantly reduced mortality in a “double-hit” mouse model of SEB-induced TSS, established via sensitization with d-galactosamine followed by SEB challenge. Therefore, our novel aptamer antagonist may offer potential therapeutic efficacy against SEB-mediated TSS. PMID:25624325

40 CFR 437.11 - Effluent limitations attainable by the application of the best practicable control technology...

Code of Federal Regulations, 2010 CFR

2010-07-01

... CENTRALIZED WASTE TREATMENT POINT SOURCE CATEGORY Metals Treatment and Recovery § 437.11 Effluent limitations... (2) (2) TSS 60.0 31.0 Metal Parameters Antimony 0.249 0.206 Arsenic 0.162 0.104 Cadmium 0.474 0.0962 Chromium 15.5 3.07 Cobalt 0.192 0.124 Copper 4.14 1.06 Lead 1.32 0.283 Mercury 0.00234 0.000739 Nickel 3.95...

40 CFR 428.55 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/kkg of raw material) Oil and grease 0.70 0.25 TSS 1.28 0.64 pH (1) (1) English units (lb/1,000 lb of raw material) Oil and grease 0.70 0.25...— Metric units (kg/kkg of raw material) Lead 0.0017 0.0007 English units (lb/1,000 lb of raw material) Lead...

40 CFR 428.55 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/kkg of raw material) Oil and grease 0.70 0.25 TSS 1.28 0.64 pH (1) (1) English units (lb/1,000 lb of raw material) Oil and grease 0.70 0.25...— Metric units (kg/kkg of raw material) Lead 0.0017 0.0007 English units (lb/1,000 lb of raw material) Lead...

40 CFR 428.105 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... consecutive days shall not exceed— Metric units (kg/kkg of raw material) Oil and grease 2.0 0.73 BOD5 3.72 2.20 TSS 6.96 2.90 pH (1) (1) English units (lb/1,000 lb of raw material) Oil and grease 2.0 0.73 BOD5... daily values for 30 consecutive days shall not exceed— Metric units (kg/kkg of raw material) Chromium 0...

40 CFR 428.105 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... consecutive days shall not exceed— Metric units (kg/kkg of raw material) Oil and grease 2.0 0.73 BOD5 3.72 2.20 TSS 6.96 2.90 pH (1) (1) English units (lb/1,000 lb of raw material) Oil and grease 2.0 0.73 BOD5... daily values for 30 consecutive days shall not exceed— Metric units (kg/kkg of raw material) Chromium 0...

40 CFR 428.65 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/kkg of raw material) Oil and grease 0.42 0.15 TSS 0.80 0.40 pH (1) (1) English units (lb/1,000 lb of raw material) Oil and grease 0.42 0.0...— Metric units (kg/kkg of raw material) Lead 0.0017 0.0007 English units (lb/1,000 lb of raw material) Lead...

40 CFR 428.65 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... values for 30 consecutive days shall not exceed— Metric units (kg/kkg of raw material) Oil and grease 0.42 0.15 TSS 0.80 0.40 pH (1) (1) English units (lb/1,000 lb of raw material) Oil and grease 0.42 0.0...— Metric units (kg/kkg of raw material) Lead 0.0017 0.0007 English units (lb/1,000 lb of raw material) Lead...

40 CFR 471.71 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2012 CFR

2012-07-01

... SOURCE CATEGORY Uranium Forming Subcategory § 471.71 Effluent limitations representing the degree of... off-pounds) of uranium extruded Cadium 0.117 0.052 Chromium 0.152 0.062 Copper 0.654 0.344 Lead 0.145 0.069 Nickel 0.661 0.437 Fluoride 20.5 9.08 Molybdenum 2.28 1.18 Oil and grease 6.88 4.13 TSS 14.1 6...

40 CFR 471.71 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2014 CFR

2014-07-01

... SOURCE CATEGORY Uranium Forming Subcategory § 471.71 Effluent limitations representing the degree of... off-pounds) of uranium extruded Cadium 0.117 0.052 Chromium 0.152 0.062 Copper 0.654 0.344 Lead 0.145 0.069 Nickel 0.661 0.437 Fluoride 20.5 9.08 Molybdenum 2.28 1.18 Oil and grease 6.88 4.13 TSS 14.1 6...

40 CFR 471.71 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2013 CFR

2013-07-01

... SOURCE CATEGORY Uranium Forming Subcategory § 471.71 Effluent limitations representing the degree of... off-pounds) of uranium extruded Cadium 0.117 0.052 Chromium 0.152 0.062 Copper 0.654 0.344 Lead 0.145 0.069 Nickel 0.661 0.437 Fluoride 20.5 9.08 Molybdenum 2.28 1.18 Oil and grease 6.88 4.13 TSS 14.1 6...

40 CFR 417.103 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Air-SO3... 0.04 .02 Surfactants 0.36 .18 Oil and grease 0.08 .04 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.30 0.19 COD 1.10 .55 TSS 0.04 .02 Surfactants 0.36 .18 Oil and grease 0...

40 CFR 417.153 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY... Surfactants 0.04 .02 Oil and grease 0.01 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.08 .04 TSS 0.04 .02 Surfactants 0.04 .02 Oil and grease 0.01 .005 pH (1) (1...

40 CFR 417.153 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY... Surfactants 0.04 .02 Oil and grease 0.01 .005 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.02 0.01 COD 0.08 .04 TSS 0.04 .02 Surfactants 0.04 .02 Oil and grease 0.01 .005 pH (1) (1...

40 CFR 417.103 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Air-SO3... 0.04 .02 Surfactants 0.36 .18 Oil and grease 0.08 .04 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.30 0.19 COD 1.10 .55 TSS 0.04 .02 Surfactants 0.36 .18 Oil and grease 0...

40 CFR 417.103 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Air-SO3... 0.04 .02 Surfactants 0.36 .18 Oil and grease 0.08 .04 pH (1) (1) English units (pounds per 1,000 lb of anhydrous product) BOD5 0.30 0.19 COD 1.10 .55 TSS 0.04 .02 Surfactants 0.36 .18 Oil and grease 0...

CapZyme-Seq Comprehensively Defines Promoter-Sequence Determinants for RNA 5' Capping with NAD.

PubMed

Vvedenskaya, Irina O; Bird, Jeremy G; Zhang, Yuanchao; Zhang, Yu; Jiao, Xinfu; Barvík, Ivan; Krásný, Libor; Kiledjian, Megerditch; Taylor, Deanne M; Ebright, Richard H; Nickels, Bryce E

2018-05-03

Nucleoside-containing metabolites such as NAD + can be incorporated as 5' caps on RNA by serving as non-canonical initiating nucleotides (NCINs) for transcription initiation by RNA polymerase (RNAP). Here, we report CapZyme-seq, a high-throughput-sequencing method that employs NCIN-decapping enzymes NudC and Rai1 to detect and quantify NCIN-capped RNA. By combining CapZyme-seq with multiplexed transcriptomics, we determine efficiencies of NAD + capping by Escherichia coli RNAP for ∼16,000 promoter sequences. The results define preferred transcription start site (TSS) positions for NAD + capping and define a consensus promoter sequence for NAD + capping: HRRASWW (TSS underlined). By applying CapZyme-seq to E. coli total cellular RNA, we establish that sequence determinants for NCIN capping in vivo match the NAD + -capping consensus defined in vitro, and we identify and quantify NCIN-capped small RNAs (sRNAs). Our findings define the promoter-sequence determinants for NCIN capping with NAD + and provide a general method for analysis of NCIN capping in vitro and in vivo. Copyright © 2018 Elsevier Inc. All rights reserved.

High voltage characteristics of the electrodynamic tether and the generation of power and propulsion

NASA Technical Reports Server (NTRS)

Williamson, P. R.

1986-01-01

The Tethered Satellite System (TSS) will deploy and retrieve a satellite from the Space Shuttle orbiter with a tether of up to 100 km in length attached between the satellite and the orbiter. The characteristics of the TSS which are related to high voltages, electrical currents, energy storage, power, and the generation of plasma waves are described. A number of specific features of the tether system of importance in assessing the operational characteristics of the electrodynamic TSS are identified.

40 CFR 436.42 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

...— TSS 45 mg/l 25 mg/l. pH (1) (1) 1 Within the range 6.0 to 9.0. (2) Except as provided in paragraphs (a... daily values for 30 consecutive days shall not exceed— TSS 0.046 0.023 Total fluoride .006 .003 pH (1... consecutive days shall not exceed— TSS 45 mg/l 25 mg/l. pH (1) (1) 1 Within the range 6.0 to 9.0. (b) Any...