Microbial Monitoring of Common Opportunistic Pathogens by Comparing Multiple Real-Time PCR Platforms for Potential Space Applications

NASA Technical Reports Server (NTRS)

Oubre, Cherie M.; Birmele, Michele N.; Castro, Victoria A.; Venkateswaran, Kasthuri J.; Vaishampayan, Parag A.; Jones, Kathy U.; Singhal, Adesh; Johnston, Angela S.; Roman, Monserrate C.; Ozbolt, Tamra A.;

Preparation and evaluation of Apollo 14 composite experiments

NASA Technical Reports Server (NTRS)

Steurer, W. H.; Kaye, S.

1971-01-01

An account is given of the work aimed at flight experiments on Apollo 14, in relation to space manufacturing processes. Evaluation of suitable materials, definition of in-flight processing procedures, preparation of preprocessed materials and delivery, and evaluation of the space-processed samples after return from the Apollo 14 flight are presented.

Sub-orbital flights, a starting point for space tourism

NASA Astrophysics Data System (ADS)

Gaubatz, William A.

2002-07-01

While there is a growing awareness and interest by the general public in space travel neither the market nor the infrastructure exist to make a commercial space tourism business an attractive risk venture. In addition there is much to be learned about how the general public will respond to space flights and what physiological and psychological needs must be met to ensure a pleasurable as well as adventurous experience. Sub-orbital flights offer an incremental approach to develop the market and the infrastructure, demonstrate the safety of space flight, obtain real flight information regarding the needs of general public passengers and demonstrate the profitability of space tourism. This paper will summarize some of the system, operations, and financial aspects of creating a sub-orbital space tourism business as a stepping-stone to public space travel. A sample business case will be reviewed and impacts of markets, operations and vehicle costs and lifetimes will be assessed.

The Apollo 16 microbial response to space environment experiment

NASA Technical Reports Server (NTRS)

Taylor, G. R.

1975-01-01

The effect was evaluated of a particular space flight on the survival rate of nine different species. Although a reasonable variety of organisms (viruses, yeasts, filamentous fungi, bacteria, and an invertebrate) were tested under several different conditions, no statistically valid differences could be detected in the survival of flight samples when compared to corresponding ground-based controls. In general, these evaluations were based on multiple observations of from ten to thirty replicates of up to one million cells each. While the results conflict with those of certain other space flight investigations, it is observed that the conditions of a particular space flight cannot be exactly duplicated, and therefore results from different flights are not directly comparable.

Changes in Monocyte Functions of Astronauts

NASA Technical Reports Server (NTRS)

Kaur, I.; Simons, E.; Castro, V.; Ott, C. Mark; Pierson, Duane L.

2004-01-01

Monocyte cell numbers and functions, including phagocytosis, oxidative burst capacity, and degranulation and expression of related surface molecules, were studied in blood specimens from 25 astronauts and 9 healthy control subjects. Blood samples were obtained 10 days before a space flight, 3 hours after landing and 3 days after landing. The number of monocytes in astronauts did not change significantly among the three sample collection periods. Following space flight, the monocytes ability to phagocytize Escherichia coli, to exhibit an oxidative burst, and to degranulate was reduced as compared to monocytes from control subjects. These alterations in monocyte functions after space flight correlated with alterations in the expression of CD32 and CD64.

Microgravity

NASA Image and Video Library

1999-06-29

Chemist Arna Holmes, left, from the University of Alabama in Huntsville, teaches NaLonda Moorer, center, and Maricar Bana, right, both from Terry Parker High School in Jacksonville, Fl, procedures for preparing protein crystal growth samples for flight aboard the International Space Station (ISS). NASA/Marshall Space Flight Center in Huntsville, AL, is a sponsor for this educational activity. The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aborad the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences (SLS) Lab, Jan Bauer, with Dynamac Corp., places samples of onion tissue in the elemental analyzer, which analyzes for carbon, hydrogen, nitrogen and sulfur. The 100,000 square-foot SLS houses labs for NASA’s ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASA’s Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASA’s Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

NASA Image and Video Library

2004-01-05

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences (SLS) Lab, Jan Bauer, with Dynamac Corp., places samples of onion tissue in the elemental analyzer, which analyzes for carbon, hydrogen, nitrogen and sulfur. The 100,000 square-foot SLS houses labs for NASA’s ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASA’s Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASA’s Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences (SLS) Lab, Jan Bauer, with Dynamac Corp., weighs samples of onion tissue for processing in the elemental analyzer behind it. The equipment analyzes for carbon, hydrogen, nitrogen and sulfur. The 100,000 square-foot SLS houses labs for NASA’s ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASA’s Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASA’s Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

NASA Image and Video Library

2004-01-05

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences (SLS) Lab, Jan Bauer, with Dynamac Corp., weighs samples of onion tissue for processing in the elemental analyzer behind it. The equipment analyzes for carbon, hydrogen, nitrogen and sulfur. The 100,000 square-foot SLS houses labs for NASA’s ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASA’s Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASA’s Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

Student Pave Way for First Microgravity Experiments on International Space Station

NASA Technical Reports Server (NTRS)

1999-01-01

Chemist Arna Holmes, left, from the University of Alabama in Huntsville, teaches NaLonda Moorer, center, and Maricar Bana, right, both from Terry Parker High School in Jacksonville, Fl, procedures for preparing protein crystal growth samples for flight aboard the International Space Station (ISS). NASA/Marshall Space Flight Center in Huntsville, AL, is a sponsor for this educational activity. The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aborad the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)

NASA Johnson Space Center Biomedical Research Resources

NASA Technical Reports Server (NTRS)

Paloski, W. H.

1999-01-01

Johnson Space Center (JSC) medical sciences laboratories constitute a national resource for support of medical operations and life sciences research enabling a human presence in space. They play a critical role in evaluating, defining, and mitigation the untoward effect of human adaption to space flight. Over the years they have developed the unique facilities and expertise required to perform: biomedical sample analysis and physiological performance tests supporting medical evaluations of space flight crew members and scientific investigations of the operationally relevant medical, physiological, cellular, and biochemical issues associated with human space flight. A general overview of these laboratories is presented in viewgraph form.

Effects of space flight on surface marker expression

NASA Astrophysics Data System (ADS)

Sonnenfeld, G.

1999-01-01

Space flight has been shown to affect expression of several cell surface markers. These markers play important roles in regulation of immune responses, including CD4 and CD8. The studies have involved flight of experimental animals and humans followed by analysis of tissue samples (blood in humans, rats and monkeys, spleen, thymus, lymph nodes and bone marrow in rodents). The degree and direction of the changes induced by space flight have been determined by the conditions of the flight. Also, there may be compartmentalization of the response of surface markers to space flight, with differences in the response of cells isolated from blood and local immune tissue. The same type of compartmentalization was also observed with cell adhesion molecules (integrins). In this case, the expression of integrins from lymph node cells differed from that of splenocytes isolated from rats immediately after space flight. Cell culture studies have indicated that there may be an inhibition in conversion of a precursor cell line to cells exhibiting mature macrophage characteristics after space flight, however, these experiments were limited as a result of technical difficulties. In general, it is clear that space flight results in alterations of cell surface markers. The biological significance of these changes remains to be established.

Reentry Capsule for Sample Return from Asteroids in the Planetary Exploration Missions

NASA Astrophysics Data System (ADS)

Inatani, Yoshifumi

2018-04-01

For carrying sample from the bodies of interplanetary space, a wide range of knowledge of reentry technology is needed. HAYABUSA(MUSES-C) was an asteroid explorer returned to the earth after the 7 years of voyage, and its capsule reenters into the Earth’s atmosphere, which was a good example of reentry technology implemented to the flight vehicle. It performed a safe reentry flight and recovery. For the design of the capsule, many considerations were made due to its higher entry velocity and higher aerodynamic heating than those of normal reentry from the low earth orbit. Taking into account the required functions throughout the orbital flight, reentry flight, and descent/recovery phase, the capsule was deigned, tested, manufactured and flight demonstrated finally. The paper presents the concept of the design and qualification approach of the small space capsule of the asteroid sample and return mission. And presented are how the reentry flight was performed and a brief overview of the post flight analysis primarily for these design validation purposes and for the better understanding of the flight results.

The effects of space radiation on flight film

NASA Technical Reports Server (NTRS)

Holly, Mark H.

1995-01-01

The Shuttle and its cargo are occasionally exposed to an amount of radiation large enough to create non-image forming exposures (fog) on photographic flight film. The television/photography working group proposed a test plan to quantify the sensitivity of photographic films to space radiation. This plan was flown on STS-37 and was later incorporated into a detailed supplementary objective (DSO) which was flown on STS48. This DSO addressed the effects of significant space radiation on representative samples of six highly sensitive flight films. In addition, a lead-lined bag was evaluated as a potential shield for flight film against space radiation.

Point of a space experiment proposal.

PubMed

Fukui, Keiji; Shimazu, Toru; Higashibata, Akira; Fujimoto, Nobuyoshi; Ishioka, Noriaki

2003-10-01

JAXA will solicit research proposals for space flight experiments that would be conducted for less than three years after the selection. In principle, available samples will be limited to Arabidopsis and C. elegans and flight hardware and protocol of space flight experiment will be pre-fixed. Proposals using different combinations of species and flight hardware will not be acceptable. Besides scientific issues, it is very important for proposer to write an impressive proposal. Hypothesis basis research proposal is the accepted standard. Reviewers will dislike a descriptive and unfocused research proposal without hypothesis. Ground preparation experiments, which are not related directly to space experiments, should not be included in the solicitation.

Effects of microgravity on renal stone risk assessment

NASA Technical Reports Server (NTRS)

Pietrzyk, R. A.; Pak, C. Y. C.; Cintron, N. M.; Whitson, P. A.

1992-01-01

Physiologic changes induced during human exposure to the microgravity environment of space may contribute to an increased potential for renal stone formation. Renal stone risk factors obtained 10 days before flight and immediately after return to earth indicated that calcium oxalate and uric acid stone-forming potential was increased after space flights of 4-10 days. These data describe the need for examining renal stone risk during in-flight phases of space missions. Because of limited availability of space and refrigerated storage on spacecraft, effective methods must be developed for collecting urine samples in-flight and for preserving (or storing) them at temperatures and under conditions commensurate with mission constraints.

Physics of Colloids in Space--Plus (PCS+) Experiment Completed Flight Acceptance Testing

NASA Technical Reports Server (NTRS)

Doherty, Michael P.

2004-01-01

The Physics of Colloids in Space--Plus (PCS+) experiment successfully completed system-level flight acceptance testing in the fall of 2003. This testing included electromagnetic interference (EMI) testing, vibration testing, and thermal testing. PCS+, an Expedite the Process of Experiments to Space Station (EXPRESS) Rack payload will deploy a second set of colloid samples within the PCS flight hardware system that flew on the International Space Station (ISS) from April 2001 to June 2002. PCS+ is slated to return to the ISS in late 2004 or early 2005.

Biochemical and hematologic changes after short-term space flight

NASA Technical Reports Server (NTRS)

Leach, Carolyn S.

1991-01-01

Clinical laboratory data from blood samples obtained from astronauts before and after 28 flights (average duration = 6 days) of the Space Shuttle were analyzed by the paired t-test and the Wilcoxon signed-rank test and compared with data from the Skylab flights (duration = 28, 56, and 84 days). Angiotensin I and aldosterone were elevated immediately after short-term space flights, but the response of angiotensin I was delayed after Skylab flights. Serum calcium was not elevated after Shuttle flights, but magnesium and uric acid decreased after both Shuttle and Skylab. Creatine phosphokinase in serum was reduced after Shuttle but not Skylab flights, probably because exercises to prevent deconditioning were not performed on the Shuttle. Total cholesterol was unchanged after Shuttle flights, but low density lipoprotein cholesterol increased and high density lipoprotein cholesterol decreased. The concentration of red blood cells was elevated after Shuttle flights and reduced after Skylab flights.

Excretion of amino acids by humans during space flight

NASA Technical Reports Server (NTRS)

Stein, T. P.; Schluter, M. D.

1998-01-01

We measured the urine amino acid distribution patterns before, during and after space flight on the Space Shuttle. The urine samples were collected on two separate flights of the space shuttle. The first flight lasted 9.5 days and the second flight 15 days. Urine was collected continuously on 8 subjects for the period beginning 10 d before launch to 6 d after landing. Results: In contrast to the earlier Skylab missions where a pronounced amino aciduria was found, on shuttle the urinary amino acids showed little change with spaceflight except for a marked decrease in all of the amino acids on FD (flight day) 1 (p<0.05) and a reduction in isoleucine and valine on FD3 and FD4 (p<0.05). Conclusions: (i) Amino aciduria is not an inevitable consequence of space flight. (ii) The occurrence of amino aciduria, like muscle protein breakdown is a mission specific effect rather than part of the general human response to microgravity.

Review of Low Earth Orbital (LEO) flight experiments

NASA Technical Reports Server (NTRS)

Leger, L.; Santosmason, B.; Visentine, J.; Kuminecz, J.

1987-01-01

The atomic oxygen flux exposure experiments flown on Space Shuttle flights STS-5 and STS-8 are described along with the results of measurements made on hardware returned from the Solar Maximum repair mission (Space Shuttle flight 41-C). In general, these experiments have essentially provided for passive exposure of samples to oxygen fluences of approximately 1 to 3.5 x 10(20) atoms/sq cm. Atmospheric density is used to derive fluence and is dependent on solar activity, which has been on the decline side of the 11-year cycle. Thus, relatively low flight altitudes of less than 300 km were used to acquire these exposures. After exposure, the samples were analyzed using various methods ranging from mass loss to extensive scanning electron microscopy and surface analysis techniques. Results are summarized and implications for the space station are discussed.

Nucleolar structure and proliferation activity of Arabidopsis root cells from seedlings germinated on the International Space Station

NASA Astrophysics Data System (ADS)

Matía, Isabel; González-Camacho, Fernando; Marco, Roberto; Kiss, John Z.; Gasset, Gilbert; Medina, Francisco-Javier

Seeds of Arabidopsis thaliana were sent to the International Space Station in the "Cervantes Mission" (Spanish Soyuz Mission). Seed germination was initiated in flight by supplying culture medium. Seedlings were grown for 4 days at 22 °C, and growth was stopped by the addition of paraformaldehyde fixative. Once back on the ground, samples were immediately processed for microscopy. A ground control experiment was simultaneously replicated. Glutaraldehyde-fixed root cells from seedlings grown in the Biorack on board of the Space Shuttle (STS-84 Mission) in similar conditions were also ultrastructurally examined. The length of seedlings grown at 1 g was conspicuously shorter than parallel samples grown under microgravity. We examined the morphology of the root meristematic cells, with a focus on their nucleoli in the cortex and stele. In general, root cortical cells proliferate at a higher rate and their nucleoli are more active than those of stele cells. While the stele showed longer cells with larger nucleoli in the flight samples, cortical cells from space-grown seedlings were shorter, more numerous and more densely packed than ground controls. However, nucleoli were smaller and less active in fast proliferating flight cells than in the ground controls. This reduced level of ribosome synthesis in the flight samples is probably the result of an accelerated cell cycle. An altered rate of cell proliferation may be detrimental for the plant and could be the reason for the reported smaller size of older space-grown seedlings. Finally, two-dimensional protein electrophoresis showed noticeable differences between space samples and ground controls.

Telescience operations with the solar array module plasma interaction experiment

NASA Technical Reports Server (NTRS)

Wald, Lawrence W.; Bibyk, Irene K.

1995-01-01

The Solar Array Module Plasma Interactions Experiment (SAMPIE) is a flight experiment that flew on the Space Shuttle Columbia (STS-62) in March 1994, as part of the OAST-2 mission. The overall objective of SAMPIE was to determine the adverse environmental interactions within the space plasma of low earth orbit (LEO) on modern solar cells and space power system materials which are artificially biased to high positive and negative direct current (DC) voltages. The two environmental interactions of interest included high voltage arcing from the samples to the space plasma and parasitic current losses. High voltage arcing can cause physical damage to power system materials and shorten expected hardware life. parasitic current losses can reduce power system efficiency because electric currents generated in a power system drain into the surrounding plasma via parasitic resistance. The flight electronics included two programmable high voltage DC power supplies to bias the experiment samples, instruments to measure the surrounding plasma environment in the STS cargo bay, and the on-board data acquisition system (DAS). The DAS provided in-flight experiment control, data storage, and communications through the Goddard Space Flight Center (GSFC) Hitchhiker flight avionics to the GSFC Payload Operations Control Center (POCC). The DAS and the SAMPIE POCC computer systems were designed for telescience operations; this paper will focus on the experiences of the SAMPIE team regarding telescience development and operations from the GSFC POCC during STS-62. The SAMPIE conceptual development, hardware design, and system verification testing were accomplished at the NASA Lewis Research Center (LeRC). SAMPIE was developed under the In-Space Technology Experiment Program (IN-STEP), which sponsors NASA, industry, and university flight experiments designed to enable and enhance space flight technology. The IN-STEP Program is sponsored by the Office of Space Access and Technology (OSAT).

Microgravity

NASA Image and Video Library

1999-06-29

Christiane Gumera, right, a student at Stanton College Preparatory High School in Jacksonville, AL, examines a protein sample while preparing an experiment for flight on the International Space Station (ISS). Merle Myers, left, a University of California, Irvine, researcher, prepares to quick-freeze protein samples in nitrogen. The proteins are in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be anlyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)

Sampling and Chemical Analysis of Potable Water for ISS Expeditions 12 and 13

NASA Technical Reports Server (NTRS)

Straub, John E. II; Plumlee, Deborah K.; Schultz, John R.

2007-01-01

The crews of Expeditions 12 and 13 aboard the International Space Station (ISS) continued to rely on potable water from two different sources, regenerated humidity condensate and Russian ground-supplied water. The Space Shuttle launched twice during the 12- months spanning both expeditions and docked with the ISS for delivery of hardware and supplies. However, no Shuttle potable water was transferred to the station during either of these missions. The chemical quality of the ISS onboard potable water supplies was verified by performing ground analyses of archival water samples at the Johnson Space Center (JSC) Water and Food Analytical Laboratory (WAFAL). Since no Shuttle flights launched during Expedition 12 and there was restricted return volume on the Russian Soyuz vehicle, only one chemical archive potable water sample was collected with U.S. hardware and returned during Expedition 12. This sample was collected in March 2006 and returned on Soyuz 11. The number and sensitivity of the chemical analyses performed on this sample were limited due to low sample volume. Shuttle flights STS-121 (ULF1.1) and STS-115 (12A) docked with the ISS in July and September of 2006, respectively. These flights returned to Earth with eight chemical archive potable water samples that were collected with U.S. hardware during Expedition 13. The average collected volume increased for these samples, allowing full chemical characterization to be performed. This paper presents a discussion of the results from chemical analyses performed on Expeditions 12 and 13 archive potable water samples. In addition to the results from the U.S. samples analyzed, results from pre-flight samples of Russian potable water delivered to the ISS on Progress vehicles and in-flight samples collected with Russian hardware during Expeditions 12 and 13 and analyzed at JSC are also discussed.

Altered cytokine production by specific human peripheral blood cell subsets immediately following space flight

NASA Technical Reports Server (NTRS)

Crucian, B. E.; Cubbage, M. L.; Sams, C. F.

2000-01-01

In this study, flow cytometry was used to positively identify the specific lymphocyte subsets exhibiting space flight-induced alterations in cytokine production. Whole blood samples were collected from 27 astronauts at three points (one preflight, two postflight) surrounding four space shuttle missions. Assays performed included serum/urine stress hormones, white blood cell (WBC) phenotyping, and intracellular cytokine production following mitogenic stimulation. Absolute levels of peripheral granulocytes were significantly elevated following space flight, but the levels of circulating lymphocytes and monocytes were unchanged. Lymphocyte subset analysis demonstrated a decreased percentage of T cells, whereas percentages of B cells and natural killer (NK) cells remained unchanged after flight. Nearly all the astronauts exhibited an increased CD4/CD8 T cell ratio. Assessment of naive (CD45RA+) vs. memory (CD45RO+) CD4+ T cell subsets was ambiguous, and subjects tended to group within specific missions. Although no significant trend was seen in absolute monocyte levels, a significant decrease in the percentage of the CD14+ CD16+ monocytes was seen following space flight in all subjects tested. T cell (CD3+) production of interleukin-2 (IL-2) was significantly decreased after space flight, as was IL-2 production by both CD4+ and CD8+ T cell subsets. Production of interferon-gamma (IFN-gamma) was not altered by space flight for the CD8+ cell subset, but there was a significant decrease in IFN-gamma production for the CD4+ T cell subset. Serum and urine stress hormone analysis indicated significant physiologic stresses in astronauts following space flight. Altered peripheral leukocyte subsets, altered serum and urine stress hormone levels, and altered T cell cytokine secretion profiles were all observed postflight. In addition, there appeared to be differential susceptibility to space flight regarding cytokine secretion by T cell subsets. These alterations may be the result of either microgravity exposure or the physiologic stresses of landing and readaptation to unit gravity. Future studies, including in-flight analysis or sampling, will be necessary to determine the cause of these alterations.

Cultivation in space flight produces minimal alterations in the susceptibility of Bacillus subtilis cells to 72 different antibiotics and growth-inhibiting compounds.

PubMed

Morrison, Michael D; Fajardo-Cavazos, Patricia; Nicholson, Wayne L

2017-08-18

Past results have suggested that bacterial antibiotic susceptibility is altered during space flight. To test this notion, Bacillus subtilis cells were cultivated in matched hardware, medium, and environmental conditions either in spaceflight microgravity on the International Space Station, termed Flight (FL) samples, or at Earth-normal gravity, termed Ground Control (GC) samples. Susceptibility of FL and GC samples was compared to 72 antibiotics and growth-inhibitory compounds using the Omnilog Phenotype Microarray (PM) system. Only 9 compounds were identified by PM screening as exhibiting significant differences ( P < 0.05, Student's t-test) in FL vs. GC samples: 6-mercaptopurine, cesium chloride, enoxacin, lomefloxacin, manganese (II) chloride, nalidixic acid, penimepicycline, rolitetracycline, and trifluoperazine. Testing of the same compounds by standard broth dilution assay did not reveal statistically significant differences in the IC 50 values between FL and GC samples. The results indicate that the susceptibility of B. subtilis cells to a wide range of antibiotics and growth inhibitors is not dramatically altered by space flight. Importance: This study addresses a major concern of mission planners for human spaceflight, that bacteria accompanying astronauts on long-duration missions might develop a higher level of resistance to antibiotics due to exposure to the spaceflight environment. The results of this study do not support that notion. Copyright © 2017 American Society for Microbiology.

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences Lab, Lanfang Levine, with Dynamac Corp., transfers material into a sample bottle for analysis. She is standing in front of new equipment in the lab that will provide gas chromatography and mass spectrometry. The equipment will enable analysis of volatile compounds, such as from plants. The 100,000 square-foot facility houses labs for NASA’s ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASA’s Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASA’s Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

NASA Image and Video Library

2004-01-05

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences Lab, Lanfang Levine, with Dynamac Corp., transfers material into a sample bottle for analysis. She is standing in front of new equipment in the lab that will provide gas chromatography and mass spectrometry. The equipment will enable analysis of volatile compounds, such as from plants. The 100,000 square-foot facility houses labs for NASA’s ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASA’s Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASA’s Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

Epidemiology of Staphylococcus aureus during space flight

NASA Technical Reports Server (NTRS)

Pierson, D. L.; Chidambaram, M.; Heath, J. D.; Mallary, L.; Mishra, S. K.; Sharma, B.; Weinstock, G. M.

1996-01-01

Staphylococcus aureus was isolated over 2 years from Space Shuttle mission crewmembers to determine dissemination and retention of bacteria. Samples before and after each mission were from nasal, throat, urine, and feces and from air and surface sampling of the Space Shuttle. DNA fingerprinting of samples by digestion of DNA with SmaI restriction endonuclease followed by pulsed-field gel electrophoresis showed S. aureus from each crewmember had a unique fingerprint and usually only one strain was carried by an individual. There was only one instance of transfer between crewmembers. Strains from interior surfaces after flight matched those of crewmembers, suggesting microbial fingerprinting may have forensic application.

Influence of Microgravity Environment on Root Growth, Soluble Sugars, and Starch Concentration of Sweetpotato Stem Cuttings

PubMed Central

Mortley, Desmond G.; Bonsi, Conrad K.; Hill, Walter A.; Morris, Carlton E.; Williams, Carol S.; Davis, Ceyla F.; Williams, John W.; Levine, Lanfang H.; Petersen, Barbara V.; Wheeler, Raymond M.

2009-01-01

Because sweetpotato [Ipomoea batatas (L.) Lam.] stem cuttings regenerate very easily and quickly, a study of their early growth and development in microgravity could be useful to an understanding of morphological changes that might occur under such conditions for crops that are propagated vegetatively. An experiment was conducted aboard a U.S. Space Shuttle to investigate the impact of microgravity on root growth, distribution of amyloplasts in the root cells, and on the concentration of soluble sugars and starch in the stems of sweetpotatoes. Twelve stem cuttings of ‘Whatley/Loretan’ sweetpotato (5 cm long) with three to four nodes were grown in each of two plant growth units filled with a nutrient agarose medium impregnated with a half-strength Hoagland solution. One plant growth unit was flown on Space Shuttle Colombia for 5 days, whereas the other remained on the ground as a control. The cuttings were received within 2 h postflight and, along with ground controls, processed in ≈45 min. Adventitious roots were counted, measured, and fixed for electron microscopy and stems frozen for starch and sugar assays. Air samples were collected from the headspace of each plant growth unit for postflight determination of carbon dioxide, oxygen, and ethylene levels. All stem cuttings produced adventitious roots and growth was quite vigorous in both ground-based and flight samples and, except for a slight browning of some root tips in the flight samples, all stem cuttings appeared normal. The roots on the flight cuttings tended to grow in random directions. Also, stem cuttings grown in microgravity had more roots and greater total root length than ground-based controls. Amyloplasts in root cap cells of ground-based controls were evenly sedimented toward one end compared with a more random distribution in the flight samples. The concentration of soluble sugars, glucose, fructose, and sucrose and total starch concentration were all substantially greater in the stems of flight samples than those found in the ground-based samples. Carbon dioxide levels were 50% greater and oxygen marginally lower in the flight plants, whereas ethylene levels were similar and averaged less than 10 nL·L −1. Despite the greater accumulation of carbohydrates in the stems, and greater root growth in the flight cuttings, overall results showed minimal differences in cell development between space flight and ground-based tissues. This suggests that the space flight environment did not adversely impact sweetpotato metabolism and that vegetative cuttings should be an acceptable approach for propagating sweetpotato plants for space applications. PMID:20186286

Investigation of periodontal tissue during a long space flights

NASA Astrophysics Data System (ADS)

Solovyeva, Zoya; Viacheslav, Ilyin; Skedina, Marina

Previous studies conducted on the International Space Station found that upon completion of the space flight there are significant changes in the local immunity and periodontal microflora of astronauts. Also research in ground-based experiments that simulate space flight factors showed that prolonged hypokinesia antiorthostatic leads to impaired functional indicators of the periodontal vascular system, an unidirectional change from the microbiota and the immune system. That results in the appearance and progressive increase of the parodontial pathogenic bacteria and increase of the content of immunoglobulins in the oral fluid. All these changes are classified as risk factors for the development of inflammatory periodontal diseases in astronauts. However, the studies were unable to determine whether the changes result from a long space flight and the peculiarities of formation the local immunity and periodontal microbiota during the space flight, or they are one of the specific manifestations of the readaptationary post-flight condition of the body. In this regard, the planned research in a long space flight suggests: to use the means of microbial control, which can retain of the anaerobes periodontal microbiota sampling directly in the space flight; to assess the specificity of changes of the periodontal immune status under the influence of the space flight factors, and to assess the state of microcirculation of periodontal tissue in astronauts. A comprehensive study of the reaction of dentition during the space flight will make it possible to study the pathogenesis of changes for developing an adequate prevention aimed at optimizing the state of dentition of the astronauts.

Microbial Monitoring of Pathogens by Comparing Multiple Real-Time PCR Platforms for Potential Space Applications

NASA Technical Reports Server (NTRS)

Birmele, Michele

2012-01-01

The International Space Station (ISS) is a closed environment wih rotations of crew and equipment each introducing their own microbial flora making it necessary to monitor the air, surfaces, and water for microbial contamination. Current microbial monitoring includes labor and time intensive methods to enumerate total bacterial and fungal cells with limited characterization during in-flight testing. Although this culture-based method has been sufficient for monitoring the ISS, future long duration missions will need to perform more comprehensive characterization in-flight, since sample return and ground characterization may not be available. A workshop was held in 2011 at the Johnson Space Center to discuss alternative methodologies and technologies suitable for microbial monitoring for these longterm exploration missions where molecular-based methodologies, such as polymerase chain reaction (PCR), were recommended. In response, a multi-center (Marshall Space Flight Center, Johnson Space Center, Jet Propulsion Laboratory, and Kennedy Space Center) collaborative research effort was initiated to explore novel commercial-off-the-shelf hardware options for spaceflight environmental monitoring. The goal was to evaluate quantitative/semi-quantitative PCR approaches to space applications for low cost in-flight rapid identification of microorganisms affecting crew safety. The initial phase of this project identified commercially available platforms that could be minimally modified to perform nominally in microgravity followed by proof-of-concept testing on the highest qualifying candidates with a universally available test organism, Salmonella enterica. The platforms evaluated during proof-of-concept testing included the iCubate 2.0(TradeMark) (iCubate, Huntsville, AL), RAZOR EX (BioFire Diagnostics; Salt Lake City, Utah) and SmartCycler(TradeMark) (Cepheid; Sunnyvale, CA). The analysis identified two potential technologies (iCubate 2.0 and RAZOR EX) that were able to perform sample-to-answer testing with cell sample concentrations between SO to 400 cells. In addition, the commercial systems were evaluated for initial flight safety and readiness, sample concentration needs were reviewed, and a competitive procurement of commercially available platforms was initiated.

Kuipers performs Water Sample Analysis

NASA Image and Video Library

2012-05-15

ISS031-E-084619 (15 May 2012) --- After collecting samples from the Water Recovery System (WRS), European Space Agency astronaut Andre Kuipers, Expedition 31 flight engineer, processes the samples for chemical and microbial analysis in the Unity node of the International Space Station.

Thirsk with SSK in JPM

NASA Image and Video Library

2009-07-15

ISS020-E-020652 (15 July 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 20 flight engineer, uses the Surface Sample Kit (SSK) to collect microbiology samples from specific sampling locations in the Harmony node and other modules of the International Space Station.

Skylab environmental and crew microbiology studies

NASA Technical Reports Server (NTRS)

Taylor, G. R.; Graves, R. C.; Brockett, R. M.; Ferguson, J. K.; Mieszkuc, B. J.

1977-01-01

Microbial flora samples were collected from crewmembers before, during, and after missions in order to obtain data on the microbial response to space flight environment. Data showed that, while gross contamination of the Skylab environment was demonstrated and there were several in-flight disease events, such events are not limiting hazards for long term manned space flights. Intercrew transfer of pathogens was demonstrated, but evidence of postflight microbial shock was not found.

Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq

PubMed Central

Wilson, J. W.; Ott, C. M.; zu Bentrup, K. Höner; Ramamurthy, R.; Quick, L.; Porwollik, S.; Cheng, P.; McClelland, M.; Tsaprailis, G.; Radabaugh, T.; Hunt, A.; Fernandez, D.; Richter, E.; Shah, M.; Kilcoyne, M.; Joshi, L.; Nelman-Gonzalez, M.; Hing, S.; Parra, M.; Dumars, P.; Norwood, K.; Bober, R.; Devich, J.; Ruggles, A.; Goulart, C.; Rupert, M.; Stodieck, L.; Stafford, P.; Catella, L.; Schurr, M. J.; Buchanan, K.; Morici, L.; McCracken, J.; Allen, P.; Baker-Coleman, C.; Hammond, T.; Vogel, J.; Nelson, R.; Pierson, D. L.; Stefanyshyn-Piper, H. M.; Nickerson, C. A.

2007-01-01

A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth. PMID:17901201

KSC-04PD-0007

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences (SLS) Lab, Jan Bauer, with Dynamac Corp., places samples of onion tissue in the elemental analyzer, which analyzes for carbon, hydrogen, nitrogen and sulfur. The 100,000 square-foot SLS houses labs for NASAs ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASAs Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASAs Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

NASA's Rodent Research Project: Validation of Capabilities for Conducting Long Duration Experiments in Space

NASA Technical Reports Server (NTRS)

Choi, Sungshin Y.; Cole, Nicolas; Reyes, America; Lai, San-Huei; Klotz, Rebecca; Beegle, Janet E.; Wigley, Cecilia L.; Pletcher, David; Globus, Ruth K.

2015-01-01

Research using rodents is an essential tool for advancing biomedical research on Earth and in space. Prior rodent experiments on the Shuttle were limited by the short flight duration. The International Space Station (ISS) provides a new platform for conducting rodent experiments under long duration conditions. Rodent Research (RR)-1 was conducted to validate flight hardware, operations, and science capabilities that were developed at the NASA Ames Research Center. Twenty C57BL6J adult female mice were launched on Sept 21, 2014 in a Dragon Capsule (SpaceX-4), then transferred to the ISS for a total time of 21-22 days (10 commercial mice) or 37 days (10 validation mice). Tissues collected on-orbit were either rapidly frozen or preserved in RNAlater at -80C (n2group) until their return to Earth. Remaining carcasses on-orbit were rapidly frozen for dissection post-flight. The three controls groups at Kennedy Space Center consisted of: Basal mice euthanized at the time of launch, Vivarium controls housed in standard cages, and Ground Controls (GC) housed in flight hardware within an environmental chamber. Upon return to Earth, there were no differences in body weights between Flight (FLT) and GC at the end of the 37 days in space. Liver enzyme activity levels of FLT mice and all control mice were similar in magnitude to those of the samples that were processed under optimal conditions in the laboratory. Liver samples dissected on-orbit yielded high quality RNA (RIN8.99+-0.59, n7). Liver samples dissected post-flight from the intact, frozen FLT carcasses yielded RIN of 7.27 +- 0.52 (n6). Additionally, wet weights of various tissues were measured. Adrenal glands and spleen showed no significant differences in FLT compared to GC although thymus and livers weights were significantly greater in FLT compared to GC. Over 3,000 tissue aliquots collected post-flight from the four groups of mice were deposited into the Ames Life Science Data Archives for future Biospecimen Sharing Program. Together, the RR validation flight successfully demonstrates the capability to support long-duration experimentation on the ISS to achieve both basic science and biomedical objectives.

Pre-Flight Characterization of Samples for the MISSE-7 Spacesuit Fabric Exposure Experiment

NASA Technical Reports Server (NTRS)

Gaier, James R.; McCue, Terry R.; Clark, Gregory W.; Rogers, Kerry J.; Mengesu, Tsega

2009-01-01

A series of six sample spacesuit pressure garment assembly (PGA) fabric samples were prepared for the Materials International Space Station Experiment 7 (MISSE-7) flight experiment to test the effects of damage by lunar dust on the susceptibility of the fabrics to radiation damage. These included pristine Apollo-era fluorinated ethylene-propylene (FEP) fabric, Apollo-era FEP fabric that had been abraded with JSC-1A lunar simulant, and a piece of Alan Bean s Apollo 12 PGA sectioned from near the left knee. Also included was a sample of pristine orthofabric, and orthofabric that had been abraded to two different levels with JSC-1A. The samples were characterized using optical microscopy, field emission scanning electron microscopy, and atomic force microscopy. Two sets of six samples were then loaded in space environment exposure hardware, one of which was stored as control samples. The other set was affixed to the MISSE-7 experiment package, and will be mounted on the International Space Station, and exposed to the wake-side low Earth orbit environment. It will be retrieved after an exposure of approximately 12 months, and returned for post flight analysis.

3D Printing in Zero-G Experiment, In Space Manufacturing (LPS, 4)

NASA Technical Reports Server (NTRS)

Bean, Quincy; Cooper, Ken; Werkheiser, Niki

2015-01-01

The 3D Printing in Zero-G Experiment has been an ongoing effort for several years. In June 2014 the technology demonstration 3D printer was launched to the International Space Station. In November 2014 the first 21 parts were manufactured in orbit marking the beginning of a paradigm shift that will allow astronauts to be more self-sufficient and pave the way to larger scale orbital manufacturing. Prior to launch the 21 parts were built on the ground with the flight unit with the same feedstock. These ground control samples are to be tested alongside the flight samples in order to determine if there is a measurable difference between parts built on the ground vs. parts built in space. As of this writing, testing has not yet commenced. Tests to be performed are structured light scanning for volume and geometric discrepancies, CT scanning for density measurement, destructive testing of mechanical samples, and SEM analysis for inter-laminar adhesion discrepancies. Additionally, an ABS material characterization was performed on mechanical samples built from the same CAD files as the flight and ground samples on different machine / feedstock combinations. The purpose of this testing was twofold: first to obtain mechanical data in order to have a baseline comparison for the flight and ground samples and second to ascertain if there is a measurable difference between machines and feedstock.

Multiple latent viruses reactivate in astronauts during Space Shuttle missions.

PubMed

Mehta, S K; Laudenslager, M L; Stowe, R P; Crucian, B E; Sams, C F; Pierson, D L

2014-10-01

Latent virus reactivation and diurnal salivary cortisol and dehydroepiandrosterone were measured prospectively in 17 astronauts (16 male and 1 female) before, during, and after short-duration (12-16 days) Space Shuttle missions. Blood, urine, and saliva samples were collected during each of these phases. Antiviral antibodies and viral load (DNA) were measured for Epstein-Barr virus (EBV), varicella-zoster virus (VZV), and cytomegalovirus (CMV). Three astronauts did not shed any virus in any of their samples collected before, during, or after flight. EBV was shed in the saliva in all of the remaining 14 astronauts during all 3 phases of flight. Seven of the 14 EBV-shedding subjects also shed VZV during and after the flight in their saliva samples, and 8 of 14 EBV-shedders also shed CMV in their urine samples before, during, and after flight. In 6 of 14 crewmembers, all 3 target viruses were shed during one or more flight phases. Both EBV and VZV DNA copies were elevated during the flight phase relative to preflight or post-flight levels. EBV DNA in peripheral blood was increased preflight relative to post-flight. Eighteen healthy controls were also included in the study. Approximately 2-5% of controls shed EBV while none shed VZV or CMV. Salivary cortisol measured preflight and during flight were elevated relative to post-flight. In contrast DHEA decreased during the flight phase relative to both preflight and post-flight. As a consequence, the molar ratio of the area under the diurnal curve of cortisol to DHEA with respect to ground (AUCg) increased significantly during flight. This ratio was unrelated to viral shedding. In summary, three herpes viruses can reactivate individually or in combination during spaceflight. Copyright © 2014 Elsevier Inc. All rights reserved.

Biochemical correlates of neurosensory changes in weightlessness

NASA Technical Reports Server (NTRS)

Leach, Carolyn S.; Reschke, Millard F.

1989-01-01

The possible existence of a relationship between space motion sickness and chemical and biochemical variables measured in body fluids is studied. Clinical chemistry and endocrine measurements from blood and urine samples taken before and after Space Shuttle flights were analyzed along with the occurrence of SMS during flight and provocative testing before flight. Significant positive correlations were observed with serum chloride and significant negative correlations with serum phosphate, serum uric acid, and plasma thyroid stimulating hormone.

Test and Analysis Capabilities of the Space Environment Effects Team at Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

Finckenor, M. M.; Edwards, D. L.; Vaughn, J. A.; Schneider, T. A.; Hovater, M. A.; Hoppe, D. T.

2002-01-01

Marshall Space Flight Center has developed world-class space environmental effects testing facilities to simulate the space environment. The combined environmental effects test system exposes temperature-controlled samples to simultaneous protons, high- and low-energy electrons, vacuum ultraviolet (VUV) radiation, and near-ultraviolet (NUV) radiation. Separate chambers for studying the effects of NUV and VUV at elevated temperatures are also available. The Atomic Oxygen Beam Facility exposes samples to atomic oxygen of 5 eV energy to simulate low-Earth orbit (LEO). The LEO space plasma simulators are used to study current collection to biased spacecraft surfaces, arcing from insulators and electrical conductivity of materials. Plasma propulsion techniques are analyzed using the Marshall magnetic mirror system. The micro light gas gun simulates micrometeoroid and space debris impacts. Candidate materials and hardware for spacecraft can be evaluated for durability in the space environment with a variety of analytical techniques. Mass, solar absorptance, infrared emittance, transmission, reflectance, bidirectional reflectance distribution function, and surface morphology characterization can be performed. The data from the space environmental effects testing facilities, combined with analytical results from flight experiments, enable the Environmental Effects Group to determine optimum materials for use on spacecraft.

Characterization of the Protein Crystal Growth Apparatus for Microgravity Aboard the Space Station

NASA Technical Reports Server (NTRS)

Kundrot, Craig E.; Roeber, D.; Achari, A.; Stinson, Thomas N. (Technical Monitor)

2002-01-01

We have conducted experiments to determine the equilibration rates of some major precipitants used in protein crystallography aboard the International Space Station (ISS). The solutions were placed in the Protein Crystallization Apparatus for Microgravity (PCAM) which mimic Cryschem sitting drop trays. The trays were placed in cylinders. These cylinders were placed inside a Single locker Thermal Enclosure System (STES), and were activated for different durations during the flight. Bumpers pressed against elastomers seal drops in a deactivated state during pre-flight and prior to transfer to the ISS. Activation occurs while in flight on the ISS by releasing the bumpers allowing the drops to be exposed to the reservoir. PCAM was flown to the ISS on STS 100, Flight 6A, on April 19, 2001. Six series of equilibration experiments were tested for each precipitant with a small amount of Green Fluorescent Protein (GFP). Cylinder 10 was never activated, 7 was activated for 40 days, 8 was activated for 20 days, 9 was activated for 10 days, 11 was activated for 4 days and 12 was activated for 2 days. Upon the return to Earth by STS 104 on July 24,2001 the samples were transferred to Marshall Space Flight Center. The samples were then brought to the lab and the volumes of each sample were measured.

Nutritional Status Assessment (SMO 016E)

NASA Technical Reports Server (NTRS)

Smith, S. M.; Heer, M. A.; Zwart, S. R.

2014-01-01

The Nutritional Status Assessment Supplemental Medical Objective was initiated to expand nominal clinical nutrition testing of ISS astronauts, and to gain a better understanding of the time course of changes in nutritional status during flight. The primary activity of this effort was collecting blood and urine samples during flight for analysis after return to Earth. Samples were subjected to a battery of tests. The resulting data provide a comprehensive survey of how nutritional status and related systems are affected by 4-6 months of space flight. Analysis of these data has yielded many findings to date, including: Vision. Documented evidence that biochemical markers involved in one-carbon metabolism were altered in crewmembers who experienced vision-related issues during and after flight (1). Iron, Oxidative Stress, and Bone. In-flight data document a clear association of increased iron stores, markers of oxidative damage to DNA, and bone loss (2). Exercise. Documented that well-nourished crewmembers performing heavy resistance exercise returned from ISS with bone mineral densities unchanged from preflight (3). Furthermore, the response of bone to space flight and exercise countermeasures was the same in men and women (4). Body Mass. Crewmembers lose 2-5% of their body mass in the first month of flight, and maintain the lower body mass during flight (5). Additionally, the two devices to measure body mass on orbit, the SLAMMD and BMMD, provide similar results (5). Cytokines. Findings indicated that a pattern of persistent physiological adaptations occurs during space flight that includes shifts in immune and hormonal regulation (6). Fish/Bone. Documented a relationship between fish intake and bone loss in astronauts (that is, those who ate more fish lost less bone) (7). Vitamin K. Documented that in generally well-fed and otherwise healthy individuals, vitamin K status and bone vitamin K-dependent proteins are unaffected by space flight (and bed rest) (8). Testosterone. Documented that blood concentrations of testosterone were unchanged during flight, but a transient decline occurred after landing (9). Calcium. Nutrition SMO data contributed to the ISS Program by helping understand how and why the Urine Processor Assembly clogged with calcium sulfate precipitate (10). Sample Processing. Ground-based analytical testing results have also been published (11).

Dietary and Urinary Sulfur can Predict Changes in Bone Metabolism During Space Flight

NASA Technical Reports Server (NTRS)

Zwart, Sara R.; Heer, Martina; Shackelford, Linda; Smith, Scott M.

2015-01-01

Mitigating space flight-induced bone loss is critical for space exploration, and diet can play a major role in this effort. Previous ground-based studies provide evidence that dietary composition can influence bone resorption during bed rest. In this study we examined the role of dietary intake patterns as one factor that can influence bone mineral loss in astronauts during space flight. Crew members were asked to consume, for 4 days at a time, prescribed menus with either a low (0.3-0.6 g/mEq) or high (1.0-1.3 g/mEq) ratio of animal protein to potassium (APro:K). Menus were developed for each crewmember, and were designed to meet both crew preferences and study constraints. Intakes of energy, total protein, calcium, and sodium were held relatively constant between the two diets. The order of the menus was randomized, and crews completed each set (low and high) once before and twice during space flight, for a total of 6 controlled diet sessions. One inflight session and three postflight sessions (R+30, R+180, R+365) monitored typical dietary intake. As of this writing, data are available from 14 crew members. The final three subjects' inflight samples are awaiting return from the International Space Station via Space-X. On the last day of each of the 4-d controlled diet sessions, 24-h urine samples were collected, along with a fasting blood sample on the morning of the 5th day. Preliminary analyses show that urinary excretion of sulfate (normalized to lean body mass) is a significant predictor of urinary n-telopeptide (NTX). Dietary sulfate (normalized to lean body mass) is also a significant predictor of urinary NTX. The results from this study, will be important to better understand diet and bone interrelationships during space flight as well as on Earth. This study was funded by the Human Health Countermeasures Element of the NASA Human Research Program.

Wakata and Thirsk with MELFI in KIBO

NASA Image and Video Library

2009-06-15

ISS020-E-010028 (15 June 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 20 flight engineer, returns a dewar tray to the Minus Eighty Laboratory Freezer for ISS (MELFI) after inserting biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight. Canadian Space Agency astronaut Robert Thirsk, flight engineer, assisted Wakata.

Gentamicin: effect on E. coli in space

NASA Technical Reports Server (NTRS)

Kacena, M. A.; Todd, P.

1999-01-01

Previous investigations have shown that liquid bacterial cultures grown in space flight were not killed as effectively by antibiotic treatments as were cultures grown on Earth. However, the cause for the decreased antibiotic effectiveness remains unknown. Possible explanations include modified cell proliferation and modified antibiotic transport in the culture medium. Escherichia coli cultures were grown in space flight (STS-69 and STS-73), with and without gentamicin, on a solid agar substrate thus eliminating fluid effects and reducing the unknowns associated with space-flight bacterial cultures in suspension. This research showed that E. coli cultures grown in flight on agar for 24 to 27 hours experienced a heightened growth compared to simultaneous controls. However, addition of gentamicin to the agar killed the bacteria such that both flight and ground control E. coli samples had similar final cell concentrations. Therefore, while the reported existence of a decrease in antibiotic effectiveness in liquid cultures remains unexplained, these data suggest that gentamicin in space flight was at least as effective as, if not more effective than, on Earth, when E. coli cells were grown on agar.

Veggie ISS Validation Test Results and Produce Consumption

NASA Technical Reports Server (NTRS)

Massa, Gioia; Hummerick, Mary; Spencer, LaShelle; Smith, Trent

2015-01-01

The Veggie vegetable production system flew to the International Space Station (ISS) in the spring of 2014. The first set of plants, Outredgeous red romaine lettuce, was grown, harvested, frozen, and returned to Earth in October. Ground control and flight plant tissue was sub-sectioned for microbial analysis, anthocyanin antioxidant phenolic analysis, and elemental analysis. Microbial analysis was also performed on samples swabbed on orbit from plants, Veggie bellows, and plant pillow surfaces, on water samples, and on samples of roots, media, and wick material from two returned plant pillows. Microbial levels of plants were comparable to ground controls, with some differences in community composition. The range in aerobic bacterial plate counts between individual plants was much greater in the ground controls than in flight plants. No pathogens were found. Anthocyanin concentrations were the same between ground and flight plants, while antioxidant and phenolic levels were slightly higher in flight plants. Elements varied, but key target elements for astronaut nutrition were similar between ground and flight plants. Aerobic plate counts of the flight plant pillow components were significantly higher than ground controls. Surface swab samples showed low microbial counts, with most below detection limits. Flight plant microbial levels were less than bacterial guidelines set for non-thermostabalized food and near or below those for fungi. These guidelines are not for fresh produce but are the closest approximate standards. Forward work includes the development of standards for space-grown produce. A produce consumption strategy for Veggie on ISS includes pre-flight assessments of all crops to down select candidates, wiping flight-grown plants with sanitizing food wipes, and regular Veggie hardware cleaning and microbial monitoring. Produce then could be consumed by astronauts, however some plant material would be reserved and returned for analysis. Implementation of this plan is a step toward developing pick-and-eat food production to supplement the packaged diet on ISS and for future exploration missions where plants could make up a larger portion of the diet. Supported by NASA Space Biology Program.

National Aeronautics and Space Administration Biological Specimen Repository

NASA Technical Reports Server (NTRS)

McMonigal, Kathleen A.; Pietrzyk, Robert a.; Johnson, Mary Anne

2008-01-01

The National Aeronautics and Space Administration Biological Specimen Repository (Repository) is a storage bank that is used to maintain biological specimens over extended periods of time and under well-controlled conditions. Samples from the International Space Station (ISS), including blood and urine, will be collected, processed and archived during the preflight, inflight and postflight phases of ISS missions. This investigation has been developed to archive biosamples for use as a resource for future space flight related research. The International Space Station (ISS) provides a platform to investigate the effects of microgravity on human physiology prior to lunar and exploration class missions. The storage of crewmember samples from many different ISS flights in a single repository will be a valuable resource with which researchers can study space flight related changes and investigate physiological markers. The development of the National Aeronautics and Space Administration Biological Specimen Repository will allow for the collection, processing, storage, maintenance, and ethical distribution of biosamples to meet goals of scientific and programmatic relevance to the space program. Archiving of the biosamples will provide future research opportunities including investigating patterns of physiological changes, analysis of components unknown at this time or analyses performed by new methodologies.

Chemical Analysis Results for Potable Water from ISS Expeditions 21 to 25

NASA Technical Reports Server (NTRS)

Straub, John E., II; Plumlee, Debrah K.; Schultz, John R.; McCoy, J. Torin

2010-01-01

The Johnson Space Center Water and Food Analytical Laboratory (WAFAL) performed detailed ground-based analyses of archival water samples for verification of the chemical quality of the International Space Station (ISS) potable water supplies for Expeditions 21 to 25. Over a 14-month period, the Space Shuttle visited the ISS on five occasions to complete construction and deliver supplies. The onboard supplies of potable water available for consumption by the Expeditions 21 to 25 crews consisted of Russian ground-supplied potable water, Russian potable water regenerated from humidity condensate, and US potable water recovered from urine distillate and condensate. Chemical archival water samples that were collected with U.S. hardware during Expeditions 21 to 25 were returned on Shuttle flights STS-129 (ULF3), STS-130 (20A), STS-131 (19A), STS-132 (ULF4) and STS-133 (ULF5), as well as on Soyuz flights 19-22. This paper reports the analytical results for the returned archival water samples and evaluates their compliance with ISS water quality standards. The WAFAL also received and analyzed aliquots of some Russian potable water samples collected in-flight and pre-flight samples of Rodnik potable water delivered to the Station on the Russian Progress vehicle during Expeditions 21 to 25. These additional analytical results are also reported and discussed in this paper.

KSC-04PD-0008

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences (SLS) Lab, Jan Bauer, with Dynamac Corp., weighs samples of onion tissue for processing in the elemental analyzer behind it. The equipment analyzes for carbon, hydrogen, nitrogen and sulfur. The 100,000 square-foot SLS houses labs for NASAs ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASAs Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASAs Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

Characterization of heat transfer in nutrient materials. [space flight feeding

NASA Technical Reports Server (NTRS)

Witte, L. C.

1985-01-01

The processing and storage of foodstuffs in zero-g environments such as in Skylab and the space shuttle were investigated. Particular attention was given to the efficient heating of foodstuffs. The thermophysical properties of various foods were cataloged and critiqued. The low temperature storage of biological samples as well as foodstuffs during shuttle flights was studied. Research and development requirements related to food preparation and storage on the space station are discussed.

Greedy Sampling and Incremental Surrogate Model-Based Tailoring of Aeroservoelastic Model Database for Flexible Aircraft

NASA Technical Reports Server (NTRS)

Wang, Yi; Pant, Kapil; Brenner, Martin J.; Ouellette, Jeffrey A.

2018-01-01

This paper presents a data analysis and modeling framework to tailor and develop linear parameter-varying (LPV) aeroservoelastic (ASE) model database for flexible aircrafts in broad 2D flight parameter space. The Kriging surrogate model is constructed using ASE models at a fraction of grid points within the original model database, and then the ASE model at any flight condition can be obtained simply through surrogate model interpolation. The greedy sampling algorithm is developed to select the next sample point that carries the worst relative error between the surrogate model prediction and the benchmark model in the frequency domain among all input-output channels. The process is iterated to incrementally improve surrogate model accuracy till a pre-determined tolerance or iteration budget is met. The methodology is applied to the ASE model database of a flexible aircraft currently being tested at NASA/AFRC for flutter suppression and gust load alleviation. Our studies indicate that the proposed method can reduce the number of models in the original database by 67%. Even so the ASE models obtained through Kriging interpolation match the model in the original database constructed directly from the physics-based tool with the worst relative error far below 1%. The interpolated ASE model exhibits continuously-varying gains along a set of prescribed flight conditions. More importantly, the selected grid points are distributed non-uniformly in the parameter space, a) capturing the distinctly different dynamic behavior and its dependence on flight parameters, and b) reiterating the need and utility for adaptive space sampling techniques for ASE model database compaction. The present framework is directly extendible to high-dimensional flight parameter space, and can be used to guide the ASE model development, model order reduction, robust control synthesis and novel vehicle design of flexible aircraft.

Embrittlement of MISSE 5 Polymers After 13 Months of Space Exposure

NASA Technical Reports Server (NTRS)

Guo, Aobo; Yi, Grace T.; Ashmead, Claire C.; Mitchell, Gianna G.; deGroh, Kim K.

2012-01-01

Understanding space environment induced degradation of spacecraft materials is essential when designing durable and stable spacecraft components. As a result of space radiation, debris impacts, atomic oxygen interaction, and thermal cycling, the outer surfaces of space materials degrade when exposed to low Earth orbit (LEO). The objective of this study was to measure the embrittlement of 37 thin film polymers after LEO space exposure. The polymers were flown aboard the International Space Station and exposed to the LEO space environment as part of the Materials International Space Station Experiment 5 (MISSE 5). The samples were flown in a nadir-facing position for 13 months and were exposed to thermal cycling along with low doses of atomic oxygen, direct solar radiation and omnidirectional charged particle radiation. The samples were analyzed for space-induced embrittlement using a bend-test procedure in which the strain necessary to induce surface cracking was determined. Bend-testing was conducted using successively smaller mandrels to apply a surface strain to samples placed on a semi-suspended pliable platform. A pristine sample was also tested for each flight sample. Eighteen of the 37 flight samples experienced some degree of surface cracking during bend-testing, while none of the pristine samples experienced any degree of cracking. The results indicate that 49 percent of the MISSE 5 thin film polymers became embrittled in the space environment even though they were exposed to low doses (approx.2.75 krad (Si) dose through 127 mm Kapton) of ionizing radiation.

Effect of Ram and Zenith Exposure on the Optical Properties of Polymers in Space

NASA Technical Reports Server (NTRS)

Li, Yuachun; de Groh, Kim K.; Banks, Bruce A.; Leneghan, Halle; Asmar, Olivia

2017-01-01

The temperature of spacecraft is influenced by the solar absorptance and thermal emittance of the external spacecraft materials. Optical and thermal properties can degrade over time in the harsh low Earth orbital (LEO) space environment where spacecraft external materials are exposed to various forms of radiation, thermal cycling, and atomic oxygen. Therefore, it is important to test the durability of spacecraft materials in the space environment. One objective of the Polymers and Zenith Polymers Experiments was to determine the effect of LEO space exposure on the optical properties of various spacecraft polymers. These experiments were flown as part of the Materials International Space Station Experiment 7 (MISSE 7) mission on the exterior of the International Space Station (ISS) for 1.5 years. Samples were flown in ram, wake or zenith directions, receiving varying amounts of atomic oxygen and solar radiation exposure. Total and diffuse reflectance and transmittance of flight and corresponding control samples were obtained post-flight using a Cary 5000 UV-Vis-NIR Spectrophotometer. Integrated air mass zero solar absorptance (s) of the flight and control samples were computed from the total transmittance and reflectance, and compared. The optical data are compared with similar polymers exposed to space for four years as part of MISSE 2, and with atomic oxygen erosion data, to help understand the degradation of these polymers in the space environment. Results show that prolonged space exposure increases the solar absorptance of some materials. Knowing which polymers remain stable will benefit future spacecraft design.

Sensitivity analysis of the space shuttle to ascent wind profiles

NASA Technical Reports Server (NTRS)

Smith, O. E.; Austin, L. D., Jr.

1982-01-01

A parametric sensitivity analysis of the space shuttle ascent flight to the wind profile is presented. Engineering systems parameters are obtained by flight simulations using wind profile models and samples of detailed (Jimsphere) wind profile measurements. The wind models used are the synthetic vector wind model, with and without the design gust, and a model of the vector wind change with respect to time. From these comparison analyses an insight is gained on the contribution of winds to ascent subsystems flight parameters.

Epstein-Barr Virus Shedding by Astronauts During Space Flight

NASA Technical Reports Server (NTRS)

Pierson, D. L.

2004-01-01

Patterns of Epstein-Barr virus (EBV) reactivation in 32 astronauts and 18 healthy age-matched control subjects were characterized by quantifying EBV shedding. Saliva samples were collected from astronauts before, during, and after 10 space shuttle missions of 5 to 14 d duration. Samples were collected on a similar schedule from control subjects. At one time point or another, EBV was detected in saliva from each of the astronauts. Of 1398 saliva specimens from 32 astronauts, polymerase chain reaction analysis showed that 314 (23%) were positive for EBV DNA. Examination by flight phase showed that 29% of the saliva specimens collected before flight were positive for EBV DNA, as were 16% of those collected during flight and 16% of those collected after flight. The mean number of copies of EBV DNA from samples taken during the flights was 417 plus or minus 31, significantly greater (p less than 0.05) than the number of copies from the preflight (40 plus or minus 2) and postflight (44 plus or minus 5) phases. In contrast, the control subjects shed EBV DNA with a frequency of 3.7% and a mean number of EBV DNA copies of 40 plus or minus 2 per mL of saliva. Ten days before flight and on landing day, titers of antibody to EBV viral capsid antigen were significantly (p less than 0.05) greater than baseline levels. On landing day, urinary levels of cortisol and catecholamines, and plasma levels of substance P and other neuropeptides, were increased over their preflight values. Increases in the number of viral copies and in the amount of EBV-specific antibody were consistent with the occurrence of EBV reactivation before, during, and after space flight.

An Updated Look at the Pro K Experiment: Urinary Acid Excretion Can Predict Changes in Bone Metabolism During Space Flight

NASA Technical Reports Server (NTRS)

Zwart, Sara R.; Heer, Martina; Shackelford, Linda; Smith, Scott M.

2015-01-01

Mitigating space flight-induced bone loss is critical for space exploration, and diet can play a major role in this effort (1). Previous ground-based studies provide evidence that dietary composition can influence bone resorption during bed rest (2). In this study we examined the role of dietary intake patterns as one factor that can influence bone mineral loss in astronauts during space flight. Crew members were asked to consume, for 4 days at a time, prescribed menus with either a low (0.3-0.6 g/mEq) or high (1.0-1.3 g/mEq) ratio of animal protein to potassium (APro:K). Menus were developed for each crewmember, and were designed to meet both crew preferences and study constraints. Intakes of energy, total protein, calcium, and sodium were held relatively constant between the two diets. The order of the menus was randomized, and crews completed each set (low and high) once before and twice during space flight, for a total of 6 controlled diet sessions. One inflight session and three postflight sessions (R+30, R+180, R+365) monitored typical dietary intake. As of this writing, data are available from 14 crew members. Two subject's samples are awaiting return from ISS via Space-X, and the final subject has one more collection session planned in November 2014. On the last day of each of the 4-d controlled diet sessions, 24-h urine samples were collected, along with a fasting blood sample on the morning of the 5th day. Preliminary analyses will show the relationships between diet and flight on markers of bone metabolism. The results from this study, which represent healthy individuals in a unique environment, will be important to better understand diet and bone interrelationships during space flight as well as on Earth. These data will be important as nutritional requirements and food systems are developed for future exploration-class missions. This study was funded by the Human Health Countermeasures Element of NASA Human Research Program.

Regulation of eIF2α phosphorylation in hindlimb-unloaded and STS-135 space-flown mice

NASA Astrophysics Data System (ADS)

Zhao, Liming; Tanjung, Nancy; Swarnkar, Gaurav; Ledet, Eric; Yokota, Hiroki

2012-09-01

Various environmental stresses elevate the phosphorylation level of eukaryotic translation initiation factor 2 alpha (eIF2α) and induce transcriptional activation of a set of stress responsive genes such as activating transcription factors 3 and 6 (ATF3 and ATF6), CCAAT/enhancer-binding protein homologous protein (CHOP), and Xbp1 (X-box binding protein 1). These stress sources include radiation, oxidation, and stress to the endoplasmic reticulum, and it is recently reported that unloading by hindlimb unloading is such a stress source. No studies, however, have examined the phosphorylation level of eIF2α (eIF2α-p) using skeletal samples that have experienced microgravity in space. In this study we addressed a question: Does a mouse tibia flown in space show altered levels of eIF2α-p? To address this question, we obtained STS-135 flown samples that were harvested 4-7 h after landing. The tibia and femur isolated from hindlimb unloaded mice were employed as non-flight controls. The effects of loading were also investigated in non- flight controls. Results indicate that the level of eIF2α-p of the non-flight controls was elevated during hindlimb unloading and reduced after being released from unloading. Second, the eIF2α-p level of space-flown samples was decreased, and mechanical loading to the tibia caused the reduction of the eIF2α-p level. Third, the mRNA levels of ATF3, ATF6, and CHOP were lowered in space-flown samples as well as in the non-flight samples 4-7 h after being released from unloading. Collectively, the results herein indicated that a release from hindlimb unloading and a return to normal weight environment from space provided a suppressive effect to eIF2α-linked stress responses and that a period of 2-4 h is sufficient to induce this suppressive outcome.

The effects of space flight on some rat liver enzymes regulating carbohydrate and lipid metabolism

NASA Technical Reports Server (NTRS)

Abraham, S.; Lin, C. Y.; Klein, H. P.; Volkmann, C.

1981-01-01

The effects of space flight conditions on the activities of certain enzymes regulating carbohydrate and lipid metabolism in rat liver are investigated in an attempt to account for the losses in body weight observed during space flight despite preflight caloric consumption. Liver samples were analyzed for the activities of 32 cytosolic and microsomal enzymes as well as hepatic glycogen and individual fatty acid levels for ground control rats and rats flown on board the Cosmos 936 biosatellite under normal space flight conditions and in centrifuges which were sacrificed upon recovery or 25 days after recovery. Significant decreases in the activities of glycogen phosphorylase, alpha-glycerol phosphate acyl transferase, diglyceride acyl transferase, aconitase and 6-phosphogluconate dehydrogenase and an increase in palmitoyl CoA desaturase are found in the flight stationary relative to the flight contrifuged rats upon recovery, with all enzymes showing alterations returning to normal values 25 days postflight. The flight stationary group is also observed to be characterized by more than twice the amount of liver glycogen of the flight centrifuged group as well as a significant increase in the ratio of palmitic to palmitoleic acid. Results thus indicate metabolic changes which may be involved in the mechanism of weight loss during weightlessness, and demonstrate the equivalence of centrifugation during space flight to terrestrial gravity.

Men and Women in Space: Bone Loss and Kidney Stone Risk after Long-Duration Space Flight

NASA Technical Reports Server (NTRS)

Smith, Scott M.; Zwart, Sara R.; Heer, Martina; Hudson, Edgar, K.; Shackelford, Linda; Morgan, Jennifer L. L.

2014-01-01

Bone loss on Earth is more prevalent in women than men, leading to the assumption that women may be at greater risk from bone loss during flight. Until recently, the number of women having flown long-duration missions was too small to allow any type of statistical analysis. We report here data from 42 astronauts on long-duration missions to the International Space Station, 33 men and 9 women. Bone mineral density (dual-energy X-ray absorptiometry), bone biochemistry (from blood and urine samples), and renal stone risk factors were evaluated before and after flight. Data were analyzed in two groups, based on available resistance exercise equipment. The response of bone mineral density to flight was the same for men and women, and the typical decrease in bone mineral density (whole body and/or regional) after flight was not observed for either sex for those using an Advanced Resistive Exercise Device. Bone biochemistry, specifically markers of formation and resorption, generally responded similarly in male and female astronauts. The response of urinary supersaturation risk to space flight was not significantly different between men and women, although risks were typically increased after flight in both groups and risks were generally greater in men than in women before and after flight. Overall, the bone and renal stone responses of men and women to space flight were not different.

Students Pave Way for First Microgravity Experiments on International Space Station

NASA Technical Reports Server (NTRS)

1999-01-01

Kim Nelson, left, of Sandalwood High School in Jacksonville, FL, helps Steven Nepowada, right, of Terry Parker High School in Jacksonville, practice loading a protein sample into a thermos-like container, known as Dewar. Students from Jacksonville worked with researchers from NASA/Marshall Space Flight Center (MSFC), as well as universities, in Huntsville, AL, on an experiment for the International Space Station (ISS). The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)

Cardiac morphology after conditions of microgravity during Cosmos 2044

NASA Technical Reports Server (NTRS)

Goldstein, Margaret A.; Edwards, Robert J.; Schroeter, John P.

1992-01-01

Light- and electron-microscopic studies were performed on cardiac muscle from rats flown on Cosmos 2044 and from four control groups. Average cross-sectional area of myofibers was measured by video analysis of the light-microscopic images of papillary and ventricular muscle samples from all animals. This cross-sectional area was significantly decreased in flight rats (P = 0.03) compared with synchronous controls. Additional findings at the electron microscopic level consistent with this atrophy were obtained by stereological analysis and optical diffraction analysis of papillary muscle samples. Slightly higher mitochondrial volume density values and mitochondria-to-myofibril ratios as well as normal A-band spacings (d1,0) and Z-band spacings of myofibrils were observed in the tail-suspension and flight groups. General morphological features similar to those in ventricular samples from the previous Cosmos 1887 flight were observed.

Sample Collection for the Russian Biodegradatsiya Experiment

NASA Image and Video Library

2007-10-01

ISS015-E-32031 (October 2007) --- Cosmonaut Oleg V. Kotov, Expedition 15 flight engineer representing Russia's Federal Space Agency, uses a Bioproby Kit to collect surface samples for analysis for the Russian Biodegradation experiment in the Zvezda Service Module of the International Space Station.

Results of the Stable Microgravity Vibration Isolation Flight Experiment

NASA Technical Reports Server (NTRS)

Edberg, Donald; Boucher, Robert; Schenck, David; Nurre, Gerald; Whorton, Mark; Kim, Young; Alhorn, Dean

1996-01-01

This paper presents an overview of the STABLE microgravity isolation system developed and successfully flight tested in October 1995. A description of the hardware design and operational principles is given. A sample of the measured flight data is presented, including an evaluation of attenuation performance provided by the actively controlled electromagnetic isolation system. Preliminary analyses of flight data show that the acceleration environment aboard STABLE's isolated platform was attenuated by a factor of more than 25 between 0.1 and 100 Hz. STABLE was developed under a cooperative agreement between National Aeronautics and Space Administration, Marshall Space Flight Center, and McDonnell Douglas Aerospace. The flight hardware was designed, fabricated, integrated, tested, and delivered to the Cape during a five month period.

The Impact of Apollo-Era Microbiology on Human Space Flight

NASA Technical Reports Server (NTRS)

Elliott, T. F; Castro, V. A.; Bruce, R. J.; Pierson, D. L.

2014-01-01

The microbiota of crewmembers and the spacecraft environment contributes significant risk to crew health during space flight missions. NASA reduces microbial risk with various mitigation methods that originated during the Apollo Program and continued to evolve through subsequent programs: Skylab, Shuttle, and International Space Station (ISS). A quarantine of the crew and lunar surface samples, within the Lunar Receiving Laboratory following return from the Moon, was used to prevent contamination with unknown extraterrestrial organisms. The quarantine durations for the crew and lunar samples were 21 days and 50 days, respectively. A series of infections among Apollo crewmembers resulted in a quarantine before launch to limit exposure to infectious organisms. This Health Stabilization Program isolated the crew for 21 days before flight and was effective in reducing crew illness. After the program developed water recovery hardware for Apollo spacecraft, the 1967 National Academy of Science Space Science Board recommended the monitoring of potable water. NASA implemented acceptability limits of 10 colony forming units (CFU) per mL and the absence of viable E. coli, anaerobes, yeasts, and molds in three separate 150 mL aliquots. Microbiological investigations of the crew and spacecraft environment were conducted during the Apollo program, including the Apollo-Soyuz Test Project and Skylab. Subsequent space programs implemented microbial screening of the crew for pathogens and acceptability limits on spacecraft surfaces and air. Microbiology risk mitigation methods have evolved since the Apollo program. NASA cancelled the quarantine of the crew after return from the lunar surface, reduced the duration of the Health Stabilization Program; and implemented acceptability limits for spacecraft surfaces and air. While microbial risks were not a main focus of the early Mercury and Gemini programs, the extended duration of Apollo flights resulted in the increased scrutiny of impact of the space flight environment on crew health. The lessons learned during that era of space flight continue to impact microbiology risk mitigation in space programs today.

Wakata and Thirsk with MELFI in KIBO

NASA Image and Video Library

2009-06-15

ISS020-E-010025 (15 June 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 20 flight engineer, removes a dewar tray from the Minus Eighty Laboratory Freezer for ISS (MELFI) in order to insert biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight. Canadian Space Agency astronaut Robert Thirsk, flight engineer, assisted Wakata.

Immune System Dysregulation, Viral Reactivation and Stress During Short-Duration Space Flight

NASA Technical Reports Server (NTRS)

Crucian, Brian; Mehta, Satish; Stowe, Raymond; Uchakin, Peter; Quiriarte, Heather; Pierson, Duane; Sams, Clarence

2010-01-01

This slide presentation reviews a study that was conducted to ascertain if the immune system dysregulation, viral reactivation and stress from short duration space flight were a result of the stress of landing and readjustment to gravity. The objectives of the study were to replace several recent immune studies with one comprehensive study that will include in-flight sampling; address lack of in-flight data: (i.e., determine the in-flight status of immunity, physiological stress, viral immunity/reactivation); determine the clinical risk related to immune dysregulation for exploration class spaceflight; and determine the appropriate monitoring strategy for spaceflight-associated immune dysfunction, that could be used for the evaluation of countermeasures.

Modeling Calcium Loss from Bones During Space Flight

NASA Technical Reports Server (NTRS)

Wastney, Meryl E.; Morukov, Boris V.; Larina, Irina M.; Abrams, Steven A.; Nillen, Jeannie L.; Davis-Street, Janis E.; Lane, Helen W.; Smith, Scott M.; Paloski, W. H. (Technical Monitor)

1999-01-01

Calcium loss from bones during space flight creates a risk for astronauts who travel into space, and may prohibit space flights to other planets. The problem of calcium loss during space flight has been studied using animal models, bed rest (as a ground-based model), and humans in-flight. In-flight studies have typically documented bone loss by comparing bone mass before and after flight. To identify changes in metabolism leading to bone loss, we have performed kinetic studies using stable isotopes of calcium. Oral (Ca-43) and intravenous (Ca-46) tracers were administered to subjects (n=3), three-times before flight, once in-flight (after 110 days), and three times post-flight (on landing day, and 9 days and 3 months after flight). Samples of blood, saliva, urine, and feces were collected for up to 5 days after isotope administration, and were analyzed for tracer enrichment. Tracer data in tissues were analyzed using a compartmental model for calcium metabolism and the WinSAAM software. The model was used to: account for carryover of tracer between studies, fit data for all studies using the minimal number of changes between studies, and calculate calcium absorption, excretion, bone calcium deposition and bone calcium resorption. Results showed that fractional absorption decreased by 50% during flight and that bone resorption and urinary excretion increased by 50%. Results were supported by changes in biochemical markers of bone metabolism. Inflight bone loss of approximately 250 mg Ca/d resulted from decreased calcium absorption combined with increased bone resorption and excretion. Further studies will assess the time course of these changes during flight, and the effectiveness of countermeasures to mitigate flight-induced bone loss. The overall goal is to enable human travel beyond low-Earth orbit, and to allow for better understanding and treatment of bone diseases on Earth.

International Space Station (ISS) 3D Printer Performance and Material Characterization Methodology

NASA Technical Reports Server (NTRS)

Bean, Q. A.; Cooper, K. G.; Edmunson, J. E.; Johnston, M. M.; Werkheiser, M. J.

2015-01-01

In order for human exploration of the Solar System to be sustainable, manufacturing of necessary items on-demand in space or on planetary surfaces will be a requirement. As a first step towards this goal, the 3D Printing In Zero-G (3D Print) technology demonstration made the first items fabricated in space on the International Space Station. From those items, and comparable prints made on the ground, information about the microgravity effects on the printing process can be determined. Lessons learned from this technology demonstration will be applicable to other in-space manufacturing technologies, and may affect the terrestrial manufacturing industry as well. The flight samples were received at the George C. Marshall Space Flight Center on 6 April 2015. These samples will undergo a series of tests designed to not only thoroughly characterize the samples, but to identify microgravity effects manifested during printing by comparing their results to those of samples printed on the ground. Samples will be visually inspected, photographed, scanned with structured light, and analyzed with scanning electron microscopy. Selected samples will be analyzed with computed tomography; some will be assessed using ASTM standard tests. These tests will provide the information required to determine the effects of microgravity on 3D printing in microgravity.

Renal stone risk assessment during Space Shuttle flights

NASA Technical Reports Server (NTRS)

Whitson, P. A.; Pietrzyk, R. A.; Pak, C. Y.

1997-01-01

PURPOSE: The metabolic and environmental factors influencing renal stone formation before, during, and after Space Shuttle flights were assessed. We established the contributing roles of dietary factors in relationship to the urinary risk factors associated with renal stone formation. MATERIALS AND METHODS: 24-hr. urine samples were collected prior to, during space flight, and following landing. Urinary and dietary factors associated with renal stone formation were analyzed and the relative urinary supersaturation of calcium oxalate, calcium phosphate (brushite), sodium urate, struvite and uric acid were calculated. RESULTS: Urinary composition changed during flight to favor the crystallization of calcium-forming salts. Factors that contributed to increased potential for stone formation during space flight were significant reductions in urinary pH and increases in urinary calcium. Urinary output and citrate, a potent inhibitor of calcium-containing stones, were slightly reduced during space flight. Dietary intakes were significantly reduced for a number of variables, including fluid, energy, protein, potassium, phosphorus and magnesium. CONCLUSIONS: This is the first in-flight characterization of the renal stone forming potential in astronauts. With the examination of urinary components and nutritional factors, it was possible to determine the factors that contributed to increased risk or protected from risk. In spite of the protective components, the negative contributions to renal stone risk predominated and resulted in a urinary environment that favored the supersaturation of stone-forming salts. Dietary and pharmacologic therapies need to be assessed to minimize the potential for renal stone formation in astronauts during/after space flight.

The Effect of Low Earth Orbit Atomic Oxygen Exposure on Phenylphosphine Oxide-Containing Polymers

NASA Technical Reports Server (NTRS)

Connell, John W.

2000-01-01

Thin films of phenylphosphine oxide-containing polymers were exposed to low Earth orbit aboard a space shuttle flight (STS-85) as part of flight experiment designated Evaluation of Space Environment and Effects on Materials (ESEM). This flight experiment was a cooperative effort between the NASA Langley Research Center (LaRC) and the National Space Development Agency of Japan (NASDA). The thin film samples described herein were part of an atomic oxygen exposure experiment (AOE) and were exposed to primarily atomic oxygen (1 X 1019 atoms/cm2). The thin film samples consisted of three phosphine oxide containing polymers (arylene ether, benzimidazole and imide). Based on post-flight analyses using atomic force microscopy, X-ray photoelectron spectroscopy, and weight loss data, it was found that atomic oxygen exposure of these materials efficiently produces a phosphate layer at the surface of the samples. This layer provides a barrier towards further attack by AO. Consequently, these materials do not exhibit linear erosion rates which is in contrast with most organic polymers. Qualitatively, the results obtained from these analyses compare favorably with those obtained from samples exposed to atomic oxygen and or oxygen plasma in ground based exposure experiments. The results of the low Earth orbit atomic oxygen exposure on these materials will be compared with those of ground based exposure to AO.

Effects of microgravity on osteoblast growth

NASA Technical Reports Server (NTRS)

Hughes-Fulford, M.; Tjandrawinata, R.; Fitzgerald, J.; Gasuad, K.; Gilbertson, V.

1998-01-01

Studies from space flights over the past two decades have demonstrated that basic physiological changes occur in humans during space flight. These changes include cephalic fluid shifts, loss of fluid and electrolytes, loss of muscle mass, space motion sickness, anemia, reduced immune response, and loss of calcium and mineralized bone. The cause of most of these manifestations is not known and until recently, the general approach was to investigate general systemic changes, not basic cellular responses to microgravity. Recently analyzed data from the 1973-1974 Skylabs disclose that there is a rise in the systemic hormone, cortisol, which may play a role in bone loss in flight. In two flights where bone growth was measured (Skylabs 3 and 4), the crew members had a significant loss of calcium accompanied by a rise in 24 hour urinary cortisol during the entire flight period. In ground-based work on osteoblasts, we have demonstrated that equivalent amounts of glucocorticoids can inhibit osteoblast cell growth. In addition, this laboratory has recently studied gene growth and activation of mouse osteoblasts (MC3T3-E1) during spaceflight. Osteoblast cells were grown on glass coverslips, loaded in the Biorack plunger boxes 18 hours before launch and activated 19 hours after launch in the Biorack incubator under microgravity conditions. The osteoblasts were launched in a serum deprived state, activated and collected in microgravity. Samples were collected at 29 hours after sera activation (0-g, n=4; 1-g, n=4). The osteoblasts were examined for changes in gene expression and cell morphology. Approximately one day after growth activation, remarkable differences were observed in gene expression in 0-g and 1-g flight samples. The 0-g activated cells had increased c-fos mRNA when compared to flight 1-g controls. The message of immediate early growth gene, cox-2 was decreased in the microgravity activated cells when compared to ground or 1-g flight controls. Cox-1 was not detected in any of the samples. There were no significant differences in the expression of actin mRNA between the 0-g and 1-g samples. These data indicate that quiescent osteoblasts are slower to enter the cell cycle in microgravity, suggesting that the force of gravity itself may be a significant factor in bone loss in spaceflight. Preliminary data from our STS 76 flight experiment support our hypothesis that a basic biological response occurs at the tissue, cellular, and molecular level in 0-g. Here we examine ground-based and space flown data on osteoblast growth in ground-based experiments mimicking space flight conditions and in microgravity to simulate lack of gravity stress to help us understand the mechanism of bone loss by experiments.

Microbiological Contamination of Spacecraft

NASA Technical Reports Server (NTRS)

Pierson, D. L.; Bruce, R. J.; Groves, T. O.; Novikova, N. D.; Viktorov, A. N.

2000-01-01

The International Space Station (ISS) Phase1 Program resulted in seven US astronauts residing aboard the Russian Space Station Mir between March 1995 and May 1998. Collaboration between U.S. and Russian scientists consisted of collection and analyses of samples from the crewmembers and the Mir and Shuttle environments before, during, and after missions that lasted from 75 to 209 days in duration. The effects of long-duration space flight on the microbial characteristics of closed life support systems and the interactions of microbes with the spacecraft environment and crewmembers were investigated. Air samples were collected using a Russian or U.S.-supplied sampler (SAS, RCS, or Burkard,) while surface samples were collected using contact slides (Hycon) or swabs. Mir recycled condensate and stored potable water sources were analyzed using the U.S.-supplied Water Experiment Kit. In-flight analysis consisted of enumeration of levels of bacteria and fungi. Amounts of microorganisms seen in the air and on surfaces were mostly within acceptability lin1its; observed temporal fluctuations in levels of microbes probably reflect changes in environmental conditions (e.g., humidity). All Mir galley hot water samples were within the standards set for Mir and the ISS. Microbial isolates were returned to Earth for identification of bacterial and fungal isolates. Crew samples (nose, throat, skin, urine, and feces) were analyzed using methods approved for the medical evaluations of Shuttle flight crews. No significant changes in crew microbiota were found during space flight or upon return relative to preflight results. Dissemination of microbes between the crew and environment was demonstrated by D A fingerprinting. Some biodegradation of spacecraft materials was observed. Accumulation of condensate allowed for the recovery of a wide range of bacteria and fungi as well as some protozoa and dust mites.

Microgravity

NASA Image and Video Library

1999-06-28

Kim Nelson, left, of Sandalwood High School in Jacksonville, FL, helps Steven Nepowada, right, of Terry Parker High School in Jacksonville, practice loading a protein sample into a thermos-like container, known as Dewar. Students from Jacksonville worked with researchers from NASA/Marshall Space Flight Center (MSFC), as well as universities, in Huntsville, AL, on an experiment for the International Space Station (ISS). The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)

Wavefront sensing in space: flight demonstration II of the PICTURE sounding rocket payload

NASA Astrophysics Data System (ADS)

Douglas, Ewan S.; Mendillo, Christopher B.; Cook, Timothy A.; Cahoy, Kerri L.; Chakrabarti, Supriya

2018-01-01

A NASA sounding rocket for high-contrast imaging with a visible nulling coronagraph, the Planet Imaging Concept Testbed Using a Rocket Experiment (PICTURE) payload, has made two suborbital attempts to observe the warm dust disk inferred around Epsilon Eridani. The first flight in 2011 demonstrated a 5 mas fine pointing system in space. The reduced flight data from the second launch, on November 25, 2015, presented herein, demonstrate active sensing of wavefront phase in space. Despite several anomalies in flight, postfacto reduction phase stepping interferometer data provide insight into the wavefront sensing precision and the system stability for a portion of the pupil. These measurements show the actuation of a 32 × 32-actuator microelectromechanical system deformable mirror. The wavefront sensor reached a median precision of 1.4 nm per pixel, with 95% of samples between 0.8 and 12.0 nm per pixel. The median system stability, including telescope and coronagraph wavefront errors other than tip, tilt, and piston, was 3.6 nm per pixel, with 95% of samples between 1.2 and 23.7 nm per pixel.

Marshall Space Flight Center Black History Month Program

NASA Image and Video Library

2018-02-21

The Black History Month program was presented at Marshall Space Flight Center with guest speaker Lt. General Stayce Harris. General Harris is the Inspector General of the Air Force and she is the first African American female Lieutenant General in the American military. The topic of her presentation was "African Americans in Times of War". The presentation was followed by an ethnic food sampling.

Estimation of Vasopressin Excretion in the Urine as a Method of Monitoring Vasopressin Secretion During Space Flight

NASA Technical Reports Server (NTRS)

Moran, W. H.

1972-01-01

It is demonstrated that, under the circumstances of space flight, the measurement of plasma ADH levels might be misleading and that only the urinary ADH levels provide reliable information. The results of a partially completed survey of ADH levels in urine samples from human subjects in which simultaneous plasma ADH levels were available are included.

The astrobiological mission EXPOSE-R on board of the International Space Station

NASA Astrophysics Data System (ADS)

Rabbow, Elke; Rettberg, Petra; Barczyk, Simon; Bohmeier, Maria; Parpart, Andre; Panitz, Corinna; Horneck, Gerda; Burfeindt, Jürgen; Molter, Ferdinand; Jaramillo, Esther; Pereira, Carlos; Weiß, Peter; Willnecker, Rainer; Demets, René; Dettmann, Jan

2015-01-01

EXPOSE-R flew as the second of the European Space Agency (ESA) EXPOSE multi-user facilities on the International Space Station. During the mission on the external URM-D platform of the Zvezda service module, samples of eight international astrobiology experiments selected by ESA and one Russian guest experiment were exposed to low Earth orbit space parameters from March 10th, 2009 to January 21st, 2011. EXPOSE-R accommodated a total of 1220 samples for exposure to selected space conditions and combinations, including space vacuum, temperature cycles through 273 K, cosmic radiation, solar electromagnetic radiation at >110, >170 or >200 nm at various fluences up to GJ m-2. Samples ranged from chemical compounds via unicellular organisms and multicellular mosquito larvae and seeds to passive radiation dosimeters. Additionally, one active radiation measurement instrument was accommodated on EXPOSE-R and commanded from ground in accordance with the facility itself. Data on ultraviolet radiation, cosmic radiation and temperature were measured every 10 s and downlinked by telemetry and data carrier every few months. The EXPOSE-R trays and samples returned to Earth on March 9th, 2011 with Shuttle flight, Space Transportation System (STS)-133/ULF 5, Discovery, after successful total mission duration of 27 months in space. The samples were analysed in the individual investigators laboratories. A parallel Mission Ground Reference experiment was performed on ground with a parallel set of hardware and samples under simulated space conditions following to the data transmitted from the flight mission.

KSC-04PD-0006

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. -- In the Space Life Sciences Lab, Lanfang Levine, with Dynamac Corp., transfers material into a sample bottle for analysis. She is standing in front of new equipment in the lab that will provide gas chromatography and mass spectrometry. The equipment will enable analysis of volatile compounds, such as from plants. The 100,000 square-foot facility houses labs for NASAs ongoing research efforts, microbiology/microbial ecology studies and analytical chemistry labs. Also calling the new lab home are facilities for space flight-experiment and flight-hardware development, new plant growth chambers, and an Orbiter Environment Simulator that will be used to conduct ground control experiments in simulated flight conditions for space flight experiments. The SLS Lab, formerly known as the Space Experiment Research and Processing Laboratory or SERPL, provides space for NASAs Life Sciences Services contractor Dynamac Corporation, Bionetics Corporation, and researchers from the University of Florida. NASAs Office of Biological and Physical Research will use the facility for processing life sciences experiments that will be conducted on the International Space Station. The SLS Lab is the magnet facility for the International Space Research Park at KSC being developed in partnership with Florida Space Authority.

Deep Charging Evaluation of Satellite Power and Communication System Components

NASA Technical Reports Server (NTRS)

Schneider, T. A.; Vaughn, J. A.; Chu, B.; Wong, F.; Gardiner, G.; Wright, K. H.; Phillips, B.

2016-01-01

A set of deep charging tests has been carried out by NASA's Marshall Space Flight Center on subscale flight-like samples developed by Space Systems/Loral, LLC. The samples, which included solar array wire coupons, a photovoltaic cell coupon, and a coaxial microwave transmission cable, were placed in passive and active (powered) circuit configurations and exposed to electron radiation. The energy of the electron radiation was chosen to deeply penetrate insulating (dielectric) materials on each sample. Each circuit configuration was monitored to determine if potentially damaging electrostatic discharge events (arcs) were developed on the coupon as a result of deep charging. The motivation for the test, along with charging levels, experimental setup, sample details, and results will be discussed.

NASA Global Atmospheric Sampling Program (GASP) data report for tape VL0014

NASA Technical Reports Server (NTRS)

Briehl, D.; Dudzinski, T. J.; Liu, D. C.

1980-01-01

The data currently available from GASP, including flight routes and dates, instrumentation, data processing procedures, and data tape specifications are described. Measurements of atmospheric ozone, cabin ozine, carbon monoxide, water vapor, particles, clouds, condensation nuclei, filter samples and related meteorological and flight information obtained during 562 flights of aircraft N533PA, N4711U, N655PA, and VH-EBE from October 3, 1977 through January 5, 1978 are reported. Data representing tropopause pressures obtained from time and space interpolation of National Meteorological Center archived data for the dates of the flights are included.

Immune Blood Sample Draw

NASA Image and Video Library

2012-04-26

ISS030-E-257690 (26 April 2012) --- European Space Agency astronaut Andre Kuipers, Expedition 30 flight engineer, prepares for IMMUNE venous blood sample draws in the Columbus laboratory of the International Space Station. Following the blood draws, the samples were temporarily stowed in the Minus Eighty Laboratory Freezer for ISS 1 (MELFI-1) and later packed together with saliva samples on the Soyuz TMA-22 for return to Earth for analysis.

Hadfield prepares to insert biological samples in the MELFI-1

NASA Image and Video Library

2013-01-07

View of Canadian Space Agency (CSA) Chris Hadfield,Expedition 34 Flight Engineer (FE),preparing to insert biological samples in the Minus Eighty Laboratory Freezer for International Space Station (ISS) - (MELFI-1),in the Japanese Experiment Module (JEM) Pressurized Module (JPM). Photo was taken during Expedition 34.

Effect of 90-day space flight (MDS-ISS) on immunological parameters in mice: lymphocyte distribution and function

NASA Astrophysics Data System (ADS)

Roberts, Arthur; Lhuillier, Andrew; Liu, Yi; Ruggiu, Alessandra; Shi, Yufang

Elucidation of the effects of space flight on the immune system of astronauts and other animal species is important for the survival and success of manned space flight, especially long-term missions. Space flight exposes astronauts to microgravity, galactic cosmic radiation (GCR), and various psycho-social stressors. Blood samples from astronauts returning from space flight have shown changes in the numbers and types of circulating leukocytes. Similarly, normal lym-phocyte homeostasis has been shown to be severely affected in mice using ground-based models of microgravity and GCR exposure, as demonstrated by profound effects on several immuno-logical parameters examined by other investigators and ourselves. In particular, lymphocyte numbers are significantly reduced and subpopulation distribution is altered in the spleen, thy-mus, and peripheral blood following hindlimb unloading (HU) in mice. Lymphocyte depletion was found to be mediated through corticosteroid-induced apoptosis, although the molecular mechanism of apoptosis induction is still under investigation. The proliferative capacity of TCR-stimulated lymphocytes was also inhibited after HU. We have similarly shown that mice exposed to high-energy 56Fe ion radiation have decreased lymphocyte numbers and perturba-tions in proportions of various subpopulations, including CD4+ and CD8+ T cells, and B cells in the spleen, and maturation stages of immature T cells in the thymus. To compare these ground-based results to the effects of actual space-flight, fresh spleen and thymus samples were recently obtained from normal and transgenic mice immediately after 90 d. space-flight in the MDS, and identically-housed ground control mice. Total leukocyte numbers in each organ were enumerated, and subpopulation distribution was examined by flow cytometric analysis of CD3, CD4, CD8, CD19, CD25, DX-5, and CD11b. Splenic T cells were stimulated with anti-CD3 and assessed for proliferation after 2-4 d., and production of several cytokines was examined using Luminex technology and quantitative PCR. These data are currently being collected and analyzed.

Spacelab 1 hematology experiment (INS103): Influence of space flight on erythrokinetics in man

NASA Technical Reports Server (NTRS)

Leach, C. S.; Chen, J. P.; Crosby, W.; Dunn, C. D. R.; Johnson, P. C.; Lange, R. D.; Larkin, E.; Tavassoli, M.

1985-01-01

An experiment conducted on the 10-day Spacelab 1 mission aboard the ninth Space Shuttle flight in November to December 1983 was designed to measure factors involved in the control of erythrocyte turnover that might be altered during weightlessness. Blood samples were collected before, during, and after the flight. Immediately after landing, red cell mass showed a mean decrease of 9.3 percent in the four astronauts. Neither hyperoxia nor an increase in blood phosphate was a cause of the decrease. Red cell survival time and iron incorporation postflight were not significantly different from their preflight levels. Serum haptoglobin did not decrease, indicating that intravascular hemolysis was not a major cause of red cell mass change. An increase in serum ferritin after the second day of flight may have been caused by red cell breakdown early in flight. Erythropoietin levels decreased during and after flight, but preflight levels were high and the decrease was not significant. The space flight-induced decrease in red cell mass may result from a failure of erythropoiesis to replace cells destroyed by the spleen soon after weightlessness is attained.

FIR ACE samples

NASA Image and Video Library

2014-06-04

ISS040-E-007368 (5 June 2014) --- NASA astronaut Reid Wiseman, Expedition 40 flight engineer, works with Advanced Colloids Experiment (ACE) samples in the Destiny laboratory of the International Space Station.

Investigation of microgravity effects on solidification phenomena of selected materials

NASA Technical Reports Server (NTRS)

Maag, Carl R.; Hansen, Patricia A.

1992-01-01

A Get Away Special (GAS) experiment payload to investigate microgravity effects on solidification phenomena of selected experimental samples has been designed for flight. It is intended that the first flight of the assembly will (1) study the p-n junction characteristics for advancing semiconductor device applications, (2) study the effects of gravity-driven convection on the growth of HgCd crystals, (3) compare the textures of the sample which crystallizes in microgravity with those found in chondrite meteorites, and (4) modify glass optical characteristics through divalent oxygen exchange. The space flight experiment consists of many small furnaces. While the experiment payload is in the low gravity environment of orbital flight, the payload controller will sequentially activate the furnaces to heat samples to their melt state and then allow cooling to resolidification in a controlled fashion. The materials processed in the microgravity environment of space will be compared to the same materials processed on earth in a one-gravity environment. This paper discusses the design of all subassemblies (furnance, electronics, and power systems) in the experiment. A complete description of the experimental materials is also presented.

Space processing applications rocket project. SPAR 8

NASA Technical Reports Server (NTRS)

Chassay, R. P. (Editor)

1984-01-01

The Space Processing Applications Rocket Project (SPAR) VIII Final Report contains the engineering report prepared at the Marshall Space Flight Center (MSFC) as well as the three reports from the principal investigators. These reports also describe pertinent portions of ground-based research leading to the ultimate selection of the flight sample composition, including design, fabrication, and testing, all of which are expected to contribute immeasurably to an improved comprehension of materials processing in space. This technical memorandum is directed entirely to the payload manifest flown in the eighth of a series of SPAR flights conducted at the White Sands Missile Range (WSMR) and includes the experiments entitled Glass Formation Experiment SPAR 74-42/1R, Glass Fining Experiment in Low-Gravity SPAR 77-13/1, and Dynamics of Liquid Bubbles SPAR Experiment 77-18/2.

Use of animal models for space flight physiology studies, with special focus on the immune system

NASA Technical Reports Server (NTRS)

Sonnenfeld, Gerald

2005-01-01

Animal models have been used to study the effects of space flight on physiological systems. The animal models have been used because of the limited availability of human subjects for studies to be carried out in space as well as because of the need to carry out experiments requiring samples and experimental conditions that cannot be performed using humans. Experiments have been carried out in space using a variety of species, and included developmental biology studies. These species included rats, mice, non-human primates, fish, invertebrates, amphibians and insects. The species were chosen because they best fit the experimental conditions required for the experiments. Experiments with animals have also been carried out utilizing ground-based models that simulate some of the effects of exposure to space flight conditions. Most of the animal studies have generated results that parallel the effects of space flight on human physiological systems. Systems studied have included the neurovestibular system, the musculoskeletal system, the immune system, the neurological system, the hematological system, and the cardiovascular system. Hindlimb unloading, a ground-based model of some of the effects of space flight on the immune system, has been used to study the effects of space flight conditions on physiological parameters. For the immune system, exposure to hindlimb unloading has been shown to results in alterations of the immune system similar to those observed after space flight. This has permitted the development of experiments that demonstrated compromised resistance to infection in rodents maintained in the hindlimb unloading model as well as the beginning of studies to develop countermeasures to ameliorate or prevent such occurrences. Although there are limitations to the use of animal models for the effects of space flight on physiological systems, the animal models should prove very valuable in designing countermeasures for exploration class missions of the future.

Validation of Procedures for Monitoring Crewmember Immune Function - Short Duration Biological Investigation

NASA Technical Reports Server (NTRS)

Sams, Clarence; Crucian, Brian; Stowe, Raymond; Pierson, Duane; Mehta, Satish; Morukov, Boris; Uchakin, Peter; Nehlsen-Cannarella, Sandra

2008-01-01

Validation of Procedures for Monitoring Crew Member Immune Function - Short Duration Biological Investigation (Integrated Immune-SDBI) will assess the clinical risks resulting from the adverse effects of space flight on the human immune system and will validate a flightcompatible immune monitoring strategy. Immune system changes will be monitored by collecting and analyzing blood, urine and saliva samples from crewmembers before, during and after space flight.

Anderson works on the Nutrition Experiment during Expedition 15

NASA Image and Video Library

2007-06-25

ISS015-E-13648 (25 June 2007) --- Astronaut Clayton Anderson, Expedition 15 flight engineer, works with test samples in the Human Research Facility 2 (HRF-2) Refrigerated Centrifuge as a part of the Nutritional Status Assessment (Nutrition) experiment in the Destiny laboratory of the International Space Station. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

Space Processing Applications Rocket (SPAR) project SPAR 7

NASA Technical Reports Server (NTRS)

Poorman, R. M.

1983-01-01

The postflight reports of each of the Principal Investigators of three selected science payloads are presented in addition to the engineering report as documented by the Marshall Space Flight Center (MSFC). Pertinent portions of ground-based research leading to the ultimate selection of the flight sample composition are described including design, fabrication and testing. Containerless processing technology, containerless processing bubble dynamics, and comparative alloy solidification are the experiments discussed.

KENNEDY SPACE CENTER, FLA. - Reporters at the dedication ceremony of a NASA hangar at the San Jose, Costa Rica, airport observe the WB-57f takeoff for its sixth Costa Rican flight. KSC and NASA researchers are testing the Aircraft-based Volcanic Emission Mass Spectrometer (AVEMS) that determines the presence and concentration of various chemicals. It is being tested in flights over the Turrialba volcano in Costa Rica, and in the crater, sampling and analyzing fresh volcanic gases in their natural chemical state. The AVEMS system has been developed for use in the Space Shuttle program, to detect toxic gas leaks and emissions in the Shuttle’s aft compartment and the crew compartment.

NASA Image and Video Library

2003-03-31

KENNEDY SPACE CENTER, FLA. - Reporters at the dedication ceremony of a NASA hangar at the San Jose, Costa Rica, airport observe the WB-57f takeoff for its sixth Costa Rican flight. KSC and NASA researchers are testing the Aircraft-based Volcanic Emission Mass Spectrometer (AVEMS) that determines the presence and concentration of various chemicals. It is being tested in flights over the Turrialba volcano in Costa Rica, and in the crater, sampling and analyzing fresh volcanic gases in their natural chemical state. The AVEMS system has been developed for use in the Space Shuttle program, to detect toxic gas leaks and emissions in the Shuttle’s aft compartment and the crew compartment.

The risk of renal stone formation during and after long duration space flight

NASA Technical Reports Server (NTRS)

Whitson, P. A.; Pietrzyk, R. A.; Morukov, B. V.; Sams, C. F.

2001-01-01

BACKGROUND: The formation of a renal stone during space flight may have serious negative effects on the health of the crewmember and the success of the mission. Urinary biochemical factors and the influence of dietary factors associated with renal stone development were assessed during long duration Mir Space Station missions. METHODS: Twenty-four-hour urine samples were collected prior to, during and following long duration space flight. The relative urinary supersaturation of calcium oxalate, calcium phosphate (brushite), sodium urate, struvite and uric acid were determined. RESULTS: Changes in the urinary biochemistry of crewmembers during long duration spaceflight demonstrated increases in the supersaturation of the stone-forming salts. In-flight hypercalciuria was evident in a number of individual crewmembers and 24-hour dietary fluid intake and urine volume were significantly lower. During flight, there was a significant increase in brushite supersaturation. CONCLUSIONS: These data suggest acute effects of space flight and postflight changes in the urinary biochemistry favoring increased crystallization in the urine. The effects of dietary intake, especially fluid intake, may have a significant impact on the potential for renal stone formation. Efforts are now underway to assess the efficacy of a countermeasure to mitigate the increased risk. Copyright 2001 S. Karger AG, Basel.

Epstein-Barr virus shedding by astronauts during space flight

NASA Technical Reports Server (NTRS)

Pierson, D. L.; Stowe, R. P.; Phillips, T. M.; Lugg, D. J.; Mehta, S. K.

2005-01-01

Patterns of Epstein-Barr virus (EBV) reactivation in 32 astronauts and 18 healthy age-matched control subjects were characterized by quantifying EBV shedding. Saliva samples were collected from astronauts before, during, and after 10 space shuttle missions of 5-14 days duration. At one time point or another, EBV was detected in saliva from each of the astronauts. Of 1398 saliva specimens from 32 astronauts, polymerase chain reaction analysis showed that 314 (23%) were positive for EBV DNA. Examination by flight phase showed that 29% of the saliva specimens collected from 28 astronauts before flight were positive for EBV DNA, as were 16% of those collected from 25 astronauts during flight and 16% of those collected after flight from 23 astronauts. The mean number of EBV copies from samples taken during the flights was 417 per mL, significantly greater (p<.05) than the number of viral copies from the preflight (40) and postflight (44) phases. In contrast, the control subjects shed EBV DNA with a frequency of 3.7% and mean number of EBV copies of 40 per mL of saliva. Ten days before flight and on landing day, titers of antibody to EBV viral capsid antigen were significantly (p<.05) greater than baseline levels. On landing day, urinary levels of cortisol and catecholamines were greater than their preflight values. In a limited study (n=5), plasma levels of substance P and other neuropeptides were also greater on landing day. Increases in the number of viral copies and in the amount of EBV-specific antibody were consistent with EBV reactivation before, during, and after space flight.

Bioavailability of Promethazine during Spaceflight

NASA Technical Reports Server (NTRS)

Boyd, Jason L.; Wang, Zuwei; Putcha, Lakshmi

2009-01-01

Promethazine (PMZ) is the choice anti-motion sickness medication for treating space motion sickness (SMS) during flight. The side effects associated with PMZ include dizziness, drowsiness, sedation, and impaired psychomotor performance which could impact crew performance and mission operations. Early anecdotal reports from crewmembers indicate that these central nervous system side effects of PMZ are absent or greatly attenuated in microgravity, potentially due to changes in pharmacokinetics (PK) and pharmacodynamics in microgravity. These changes could also affect the therapeutic effectiveness of drugs in general and PMZ, in particular. In this investigation, we examined bioavailability and associated pharmacokinetics of PMZ in astronauts during and after space flight. Methods. Nine astronauts received, per their preference, PMZ (25 or 50 mg as intramuscular injection, oral tablet, or rectal suppository) on flight day one for the treatment of SMS and subsequently collected saliva samples and completed sleepiness scores for 72 h post dose. Thirty days after the astronauts returned to Earth, they repeated the protocol. Bioavailability and PK parameters were calculated and compared between flight and ground. Results. Maximum concentration (Cmax) was lower and time to reach Cmax (tmax) was longer in flight than on the ground. Area under the curve (AUC), a measure of bioavailability, was lower and biological half-life (t1/2) was longer in flight than on the ground. Conclusion. Results indicate that bioavailability of PMZ is reduced during spaceflight. Number of samples, sampling method, and sampling schedule significantly affected PK parameter estimates.

Biochemical and hematologic changes after short-term space flight

NASA Technical Reports Server (NTRS)

Leach, C. S.

1992-01-01

Clinical laboratory data from blood samples obtained from astronauts before and after 28 flights (average duration = 6 days) of the Space Shuttle were analyzed by the paired t-test and the Wilcoxon signed-rank test and compared with data from the Skylab flights (duration approximately 28, 59, and 84 days). Angiotensin I and aldosterone were elevated immediately after short-term space flights, but the response of angiotensin I was delayed after Skylab flights. Serum calcium was not elevated after Shuttle flights, but magnesium and uric acid decreased after both Shuttle and Skylab. Creatine phosphokinase in serum was reduced after Shuttle but not Skylab flights, probably because exercises to prevent deconditioning were not performed on the Shuttle. Total cholesterol was unchanged after Shuttle flights, but low density lipoprotein cholesterol increased and high density lipoprotein cholesterol decreased. The concentration of red blood cells was elevated after Shuttle flights and reduced after Skylab flights. Reticulocyte count was decreased after both short- and long-term flights, indicating that a reduction in red blood cell mass is probably more closely related to suppression of red cell production than to an increase in destruction of erythrocytes. Serum ferritin and number of platelets were also elevated after Shuttle flights. In determining the reasons for postflight differences between the shorter and longer flights, it is important to consider not only duration but also countermeasures, differences between spacecraft, and procedures for landing and egress.

Cytogenetic effects of space radiation in lymphocytes of MIR-18 crews

NASA Technical Reports Server (NTRS)

Yang, T. C.; George, K.; Johnson, A. S.; Tavakoli, A.; Durante, M.; Fedorenko, B. S.

1997-01-01

For assessing health risk, the measurement of physical dose received during a space mission, as well as the LETs, energies and charges of particles is important. It is also important to obtain quantitative information regarding the effectiveness of space radiation in causing damage to critical biological targets, e.g., chromosomes, since at present the estimated uncertainty of biological effects of space radiation is more than a factor of two. Such large uncertainty makes accurate health risk assessment very difficult. For this very reason, a study on cytogenetic effects of space radiation in human lymphocytes was proposed and done for MIR-18 mission. This study used FISH technique to score chromosomal translocations and C-banding method to determine dicentrics. Growth kinetics of cells and SCE were examined to ensure that chromosomal aberrations were scored in first mitosis and were induced not by chemical mutagens. Our results showed that chromosomal aberration frequency of post-flight samples was significantly higher than that of pre-flight ones and that SCE frequency was similar between pre- and post-flight samples. Based on a dose-response curve of preflight samples exposed to gamma rays, the absorbed dose received by crews during the mission was estimated to be about 14.5 cSv. Because the absorbed dose measured by physical dosimeters is 4.16 cGy for the entire mission, the RBE is about 3.5.

Stratospheric experiments on curing of composite materials

NASA Astrophysics Data System (ADS)

Chudinov, Viacheslav; Kondyurin, Alexey; Svistkov, Alexander L.; Efremov, Denis; Demin, Anton; Terpugov, Viktor; Rusakov, Sergey

2016-07-01

Future space exploration requires a large light-weight structure for habitats, greenhouses, space bases, space factories and other constructions. A new approach enabling large-size constructions in space relies on the use of the technology of polymerization of fiber-filled composites with a curable polymer matrix applied in the free space environment on Erath orbit. In orbit, the material is exposed to high vacuum, dramatic temperature changes, plasma of free space due to cosmic rays, sun irradiation and atomic oxygen (in low Earth orbit), micrometeorite fluence, electric charging and microgravitation. The development of appropriate polymer matrix composites requires an understanding of the chemical processes of polymer matrix curing under the specific free space conditions to be encountered. The goal of the stratospheric flight experiment is an investigation of the effect of the stratospheric conditions on the uncured polymer matrix of the composite material. The unique combination of low residual pressure, high intensity UV radiation including short-wave UV component, cosmic rays and other aspects associated with solar irradiation strongly influences the chemical processes in polymeric materials. We have done the stratospheric flight experiments with uncured composites (prepreg). A balloon with payload equipped with heater, temperature/pressure/irradiation sensors, microprocessor, carrying the samples of uncured prepreg has been launched to stratosphere of 25-30 km altitude. After the flight, the samples have been tested with FTIR, gel-fraction, tensile test and DMA. The effect of cosmic radiation has been observed. The composite was successfully cured during the stratospheric flight. The study was supported by RFBR grants 12-08-00970 and 14-08-96011.

Autoflora in the upper respiratory tract of Apollo astronauts.

PubMed Central

Decelle, J G; Taylor, G R

1976-01-01

The typical microbial inhabitants of the oral and nasal cavities of Apollo astronauts were identified before space flight and generally found to be similar to those previously reported for healthy male adults. Additional analyses of samples collected immediately after return of the Apollo 13, 14, 15, and 16 crew members to earth were performed to evaluate the effects of space travel on the microbial bioburden of the upper respiratory tract. In-flight cross-contamination and buildup of pathogens such as Staphylococcus aureus were noted, although significant increases in nonpathogenic species were absent. Other proposed alterations, such as dysbacteriosis (flooding of the mouth with a single species) and simplification of the autoflora, did not occur. Generally, the incidence and quantitation of each species after flight was within the preflight range, although the number of viable Haemophilus cells recovered from the mouth decreased significantly after space flight. Except for those minor alterations listed above, the aerobic and anaerobic bacterial components of the upper respiratory autoflora of Apollo astronauts was found to be stable after space flight of up to 295 h. PMID:984836

The influence of space flight on erythrokinetics in man. Space Life Sciences Missions 1 and 2. Experiment E261

NASA Technical Reports Server (NTRS)

Alfrey, Clarence P.

1995-01-01

The purpose of this contract was to design and conduct experiments that would increase our understanding of the influence of space flight on erythrokinetics and the rapid change that occurs in the red blood cell mass during spaceflight. The experiment designated E261, was flown on Space Life Science missions SLS-1 and SLS-2 (STS 40 and STS 58). Unique features of this experiment included radionuclide tracer studies during flight and frequent in-flight blood samples specifically for the first three or four days of the mission. Plasma volume measurements were made early and late in the missions. Radioactive iron kinetics studies were initiated after one or three days in microgravity since the magnitude of the red blood cell mass decrease dictated that bone marrow production must be decreased very early in the flight. The schedule was designed to study the time course of the changes that occur during spaceflight and to possibly define a mechanism for the rapid reduction in red blood cell mass.

Manual for LDEF tensile tests

NASA Technical Reports Server (NTRS)

Witte, W. G., Jr.

1985-01-01

One of the experiments aboard the NASA Long Duration Exposure Facility (LDEF) consists of a tray of approximately one hundred tensile specimens of several candidate space structure composite materials. During the LDEF flight the materials will be subjected to the space environment and to possible contamination during launch and recovery. Tensile tests of representative samples were made before the LDEF flight to obtain baseline data. Similar tests will be made on control specimens stored on earth for the length of the LDEF flight and on recovered flight specimens. This manual codifies the details of testing, data acquisition, and handling used in obtaining the baseline data so that the same procedures and equipment will be used on the subsequent tests.

Bone Density Following Three Years of Recovery from Long-Duration Space-Flight

NASA Technical Reports Server (NTRS)

Amin, S.; Achenbach, S. J.; Atkinson, E. J.; Sibonga, J.

2010-01-01

Bone loss during long-duration space flight is well recognized, but the long-term implications on bone health following return from flight remain unclear. Among US crew who were involved in long-duration missions in space (Mir and ISS), we have previously shown that at approximately 12 months following return, men, but not women, had BMD values at most sites that were still lower than would be expected had they not been exposed to a prolonged period of microgravity. We now extend our observations to 3 years of follow-up post-flight. Using their age, pre-flight BMD and follow-up time, post-flight BMD values for each US crew were predicted based on the model developed from the community sample. We found BMD measures to be either stable or improve by 3 years relative to their immediate post-flight BMD, however only total hip BMD still remains significantly lower than would be expected had they not been exposed to microgravity. Among male US crew, who have had their BMD measured following at least 3 years of recovery post long-duration flight, they continue to have lower BMD at the hip than would be expected, raising potential concerns regarding future hip fracture risk.

Thirsk inserts samples into MELFI in the JPM

NASA Image and Video Library

2009-06-07

ISS020-E-007156 (7 June 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

Global Properties of X-Ray Flashes and X-Ray-Rich Gamma-Ray Bursts Observed by Swift

NASA Astrophysics Data System (ADS)

Sakamoto, Takanori; Yamazaki, Ryo; Barthelmy, Scott; Gehrels, Neil; Osborne, Julian; Hullinger, Derek; Sato, Goro; Barbier, Louis; Cummings, Jay; Fenimore, Ed; Krimm, Hans; Lamb, Don; Markwardt, Craig; Palmer, David; Parsons, Ann; Stamatikos, Michael; Tueller, Jack

Takanori Sakamoto, Taka.Sakamoto@nasa.gov NASA Goddard Space Flight Center, Greenbelt, Maryland, United States Ryo Yamazaki, ryo@theo.phys.sci.hiroshima-u.ac.jp Hiroshima University, Higashi-Hiroshima, Japan Scott Barthelmy, scott@milkyway.gsfc.nasa.gov NASA GSFC, Greenbelt, Maryland, United States Neil Gehrels, gehrels@milkyway.gsfc.nasa.gov NASA Goddard Space Flight Center, Greenbelt, Maryland, United States Julian Osborne, julo@star.le.ac.uk University of Leicester, Leicester, United Kingdom Derek Hullinger, derek.hullinger@gmail.com Moxtek, Inc, Orem, Utah, United States Goro Sato, Goro.Sato@nasa.gov Goddard Space Flight Center, Greenbelt, Maryland, United States Louis Barbier, lmb@milkyway.gsfc.nasa.gov Goddard Space Flight Center, Greenbelt, Maryland, United States Jay Cummings, jayc@milkyway.gsfc.nasa.gov Goddard Space Flight Center, Greenbelt, Maryland, United States Ed Fenimore, efenimore@lanl.gov Los Alamos National Laboratory, Los Alamos, California, United States Hans Krimm, hans.krimm@nasa.gov Goddard Space Flight Center, Greenbelt, Maryland, United States Don Lamb, d-lamb@uchicago.edu University of Chicago, Chicago, Illinois, United States Craig Markwardt, Craig.Markwardt@nasa.gov Goddard Space Flight Center, Greenbelt, Maryland, United States David Palmer, palmer@lanl.gov Los Alamos National Laboratory, Los Alamos, California, United States Ann Parsons, Ann.M.Parsons@nasa.gov Goddard Space Flight Center, Greenbelt, Maryland, United States Michael Stamatikos, michael@milkyway.gsfc.nasa.gov Goddard Space Flight Center, Greenbelt, Maryland, United States Jack Tueller, jack.tueller@nasa.gov Goddard Space Flight Center, Greenbelt, Maryland, United States We present the spectral and temporal characteristics of the prompt emission and X-ray afterglow emission of X-ray flashes (XRFs) and X-ray-rich gamma-ray bursts (XRRs) detected and observed by Swift between December 2004 and September 2006. We compare these characteristics to a sample of conventional classical gamma-ray bursts (C-GRBs) observed during the same period. We confirm the correlation between Epeak and fluence noted by others and find further evidence that XRFs, XRRs and C-GRBs form a continuum. We also confirm that our known redshift sample is consistent with the correlation between the peak energy in the GRB rest frame (Epeak) and the isotropic radiated energy (Eiso), so called the Epeak-Eiso relation. The spectral properties of X-ray afterglows of XRFs and C-GRBs are similar, but the temporal properties of XRFs and C-GRBs are quite different. We found that the light curves of C-GRB afterglows show a break to steeper indices (shallow-to-steep break) at much earlier times than do XRF afterglows. Moreover, the overall luminosity of XRF X-ray afterglows is systematically smaller by a factor of two or more compared to that of C-GRBs. These distinct differences between the X-ray afterglows of XRFs and C-GRBs may be the key to understanding not only the mysterious shallow-to-steep break in X-ray afterglow light curves, but also the unique nature of XRFs.

Williams loads the HRF2 Refrigerated Centrifuge for the Nutrition Experiment during Expedition 15

NASA Image and Video Library

2007-06-01

ISS015-E-10554 (1 June 2007) --- Astronaut Sunita L. Williams, Expedition 15 flight engineer, loads test samples in the Human Research Facility 2 (HRF-2) Refrigerated Centrifuge as a part of the Nutritional Status Assessment (Nutrition) experiment in the Destiny laboratory of the International Space Station. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

Increased Renal Solute Excretion in Rats Following Space Flight

NASA Technical Reports Server (NTRS)

Wade, Charles E.; Moore, A. L.; Morey-Holton, E.

1995-01-01

Following space flight a diuresis, due to an increase in free water clearance, has been suggested in humans. To assess the effects of space flight on renal function, rats were flown in space for 14 days. Rats were divided into three groups; vivarium controls (V;n=6; housed 2/shoe box cage), flight controls (FC;n=6; group housed in a flight cage), and flight animals (F;n=6). Upon landing all animals were placed into individual metabolic cages. Urine was collected daily for 7 days and every other day for 14 days. Urine output was increased (p less than 0.05; ANOVA) following flight for 3 days. On postflight day 1, flow rates were, V=6.8 plus or minus 0.9, FC=8.711.8 and F=16.6 plus or minus 2.7 microliter/min. Excretion rates of Na+ and K+ were increased, resulting in an increased osmotic excretion rate (V=7.9 plus or minus 0.9, FC=6.1 plus or minus 0.7 and F=13.5 plus or minus 0.7 uOsm/min). Creatinine excretion rate was increased over the first two postflight days. In the absence of changes in plasma creatinine, Na+, or K+ (samples obtained immediately post flight from similar rats compared to Day 14), GFR was increased following space flight. The increased excretion of solute was thus the result of increased delivery and decreased reabsorption. Osmotic clearance was increased (V=28, FC=27 and F=51 microliter/min), while free water clearance was decreased post flight (V=-21,FC=-18 and F=-34 microliter/min). In rats, the postflight diuresis is the result of an increase in solute (osmotic) excretion with an accompanying reduction in free water clearance.

Optical Measurements on Solid Specimens of Solid Rocket Motor Exhaust and Solid Rocket Motor Slag

NASA Technical Reports Server (NTRS)

Roberts, F. E., III

1991-01-01

Samples of aluminum slag were investigated to aid the Earth Science and Applications Division at the Marshall Space Flight Center (MSFC). Alumina from space motor propellant exhaust and space motor propellant slag was examined as a component of space refuse. Thermal emittance and solar absorptivity measurements were taken to support their comparison with reflectance measurements derived from actual debris. To determine the similarity between the samples and space motor exhaust or space motor slag, emittance and absorbance results were correlated with an examination of specimen morphology.

Space Processing Applications Rocket (SPAR) project: SPAR 10

NASA Technical Reports Server (NTRS)

Poorman, R. (Compiler)

1986-01-01

The Space Processing Applications Rocket Project (SPAR) X Final Report contains the compilation of the post-flight reports from each of the Principal Investigators (PIs) on the four selected science payloads, in addition to the engineering report as documented by the Marshall Space Flight Center (MSFC). This combined effort also describes pertinent portions of ground-based research leading to the ultimate selection of the flight sample composition, including design, fabrication and testing, all of which are expected to contribute to an improved comprehension of materials processing in space. The SPAR project was coordinated and managed by MSFC as part of the Microgravity Science and Applications (MSA) program of the Office of Space Science and Applications (OSSA) of NASA Headquarters. This technical memorandum is directed entirely to the payload manifest flown in the tenth of a series of SPAR flights conducted at the White Sands Missile Range (WSMR) and includes the experiments entitled, Containerless Processing Technology, SPAR Experiment 76-20/3; Directional Solidification of Magnetic Composites, SPAR Experiment 76-22/3; Comparative Alloy Solidification, SPAR Experiment 76-36/3; and Foam Copper, SPAR Experiment 77-9/1R.

Validation of Procedures for Monitoring Crewmember Immune Function

NASA Technical Reports Server (NTRS)

Crucian, Brian; Stowe, Raymond; Mehta, Satish; Uchakin, Peter; Quiriarte, Heather; Pierson, Duane; Sams, Clarence

2008-01-01

There is ample evidence to suggest that space flight leads to immune system dysregulation. This may be a result of microgravity, confinement, physiological stress, radiation, environment or other mission-associated factors. The clinical risk (if any) from prolonged immune dysregulation during exploration-class space flight has not yet been determined, but may include increased incidence of infection, allergy, hypersensitivity, hematological malignancy or altered wound healing. Each of the clinical events resulting from immune dysfunction has the potential to impact mission critical objectives during exploration-class missions. To date, precious little in-flight immune data has been generated to assess this phenomenon. The majority of recent flight immune studies have been post-flight assessments, which may not accurately reflect the in-flight status of immunity as it resolves over prolonged flight. There are no procedures currently in place to monitor immune function or its effect on crew health. The objective of this Supplemental Medical Objective (SMO) is to develop and validate an immune monitoring strategy consistent with operational flight requirements and constraints. This SMO will assess immunity, latent viral reactivation and physiological stress during both short and long duration flights. Upon completion, it is expected that any clinical risks resulting from the adverse effects of space flight on the human immune system will have been determined. In addition, a flight-compatible immune monitoring strategy will have been developed with which countermeasures validation could be performed. This study will determine, to the best level allowed by current technology, the in-flight status of crewmembers' immune systems. The in-flight samples will allow a distinction between legitimate in-flight alterations and the physiological stresses of landing and readaptation which are believed to alter R+0 assessments. The overall status of the immune system during flight (activation, deficiency, dysregulation) and the response of the immune system to specific latent virus reactivation (known to occur during space flight) will be thoroughly assessed. The first in-flight activity for integrated immunity very recently occurred during the STS-120 Space Shuttle mission. The protocols functioned well from a technical perspective, and accurate in-flight data was obtained from 1 Shuttle and 2 ISS crewmembers. Crew participation rates for the study continue to be robust.

Barratt collects sample from WRS

NASA Image and Video Library

2009-05-19

ISS019-E-017918 (19 May 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, collects a sample from the Water Recovery System (WRS) in the Destiny laboratory of the International Space Station.

Identification of Unknown Contaminants in Water Samples from ISS Employing Liquid Chromatography/Mass Spectrometry/Mass Spectrometry

NASA Technical Reports Server (NTRS)

Rutz, Jeffrey A.; Schultz, John R.

2008-01-01

Mass Spectrometry/Mass Spectrometry (MS/MS) is a powerful technique for identifying unknown organic compounds. For non-volatile or thermally unstable unknowns dissolved in liquids, liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) is often the variety of MS/MS used for the identification. One type of LC/MS/MS that is rapidly becoming popular is time-of-flight (TOF) mass spectrometry. This technique is now in use at the Johnson Space Center for identification of unknown nonvolatile organics in water samples from the space program. An example of the successful identification of one unknown is reviewed in detail in this paper. The advantages of time-of-flight instrumentation are demonstrated through this example as well as the strategy employed in using time-of-flight data to identify unknowns.

Attitudes towards personal and shared space during the flight.

PubMed

Ahmadpour, N; Kühne, M; Robert, J-M; Vink, P

2016-07-25

Aircraft passenger comfort experience was previously defined based on its underlying thematic components representing passengers' perception of the environmental elements and their link to their concerns. This paper aims to 1) identify aircraft passengers' attitudes towards their personal and shared space in the cabin environment during the flight which are linked to their comfort experience and 2) highlight passenger concerns associated with those attitudes. A sample involving 16 participants was conducted, collecting full accounts of their real-time flight experiences onboard commercial aircrafts, using questionnaires. Four types of attitudes were identified in reaction to participants' personal and shared space during the flight. Those were described as adjust, avoid, approach, and shield. Passengers' concerns associated with those attitudes were respectively: control, privacy, connectedness and tolerance. It is concluded that passenger comfort can be improved once the identified concerns and attitudes are addressed in the design of the aircraft seat and interior. Design recommendations are provided accordingly.

Spaceflight Effects and Molecular Responses in the Mouse Eye: Observations After Shuttle Mission STS-133

NASA Technical Reports Server (NTRS)

Prospero-Ponce, Claudia; Zanello, Susana B.; CoreyTheriot, Patricia; Chevez-Barrios, P.

2012-01-01

Microgravity-induced cephalad fluid shift and radiation exposure are some of the stressors seen in space exploration. Ocular changes leading to visual impairment in astronauts are of occupational health relevance. Therefore, we analyzed the effects of space flight in the eyes of mice. Six mice were assigned to Flight (FLT), Animal enclosure Module (AEM), or vivarium (VIV) group, respectively. Mice were sacrificed at 1, 5 or 7 days after landing from space. One eye was used for histological and immunohistoche-mistry analysis and the other eye for gene expression profiling. 8-OHdG and caspase-3 immunoreactivity were increased in the retina in FLT samples at return(R+1) compared to AEM/VIV groups, and decreased at day 7 (R+7). beta-amyloid was seen in the nerve fibers at the post-laminar region of the optic nerve in the flight samples (R+7). In addition, oxidative and cellular stress response genes were upregulated in the retina of FLT samples upon landing, and decreased by R+7. According to the results, a reversible molecular damage may occur in the retina of mice exposed to spaceflight followed by protective cellular response.

Spacecraft Environment May Reduce Resistance To Infection

NASA Technical Reports Server (NTRS)

Pierson, Duane L.; Ott, C. Mark; Castro, V. A.; Leal, Melanie; Mehta, Satish K.

2006-01-01

Living and working in a spacecraft exposes the crew to a unique environment. This environment includes microgravity, increased radiation, chemical and biological contamination, and a variety of stressors. Disturbances in this balance are often manifested by diminished immunity in astronauts/cosmonauts. Reactivation of Epstein- Barr virus (EBV), cytomegalovirus (CMV), and varicella-zoster virus (VZV) has been used as an indicator of immune status. Reactivation of EBV and VZV were detected and quantified in saliva. CMV was measured in urine. The DNA was extracted using a Qiagen Inc. kit and viral DNA was detected by real time polymerase chain reaction (PCR) based assay with Taqman 7700 (PE Biosystems). Patterns of Epstein-Barr virus (EBV) reactivation in 32 astronauts and 18 healthy age-matched control subjects were characterized by quantifying EBV shedding. Saliva samples were collected before, during, and after 10 space shuttle missions of 5 to 14 d duration. Of 1398 saliva specimens from 32 astronauts, 314 (23%) were positive for EBV DNA. Examination by flight phase showed that 29% of the saliva specimens collected from 28 astronauts before flight were positive for EBV DNA, as were 16% of those collected from 25 astronauts during flight and 16% of those collected after flight from 23 astronauts. The mean number of EBV copies/mL from samples taken during the flights was 417, ten-fold greater (p < 0.05) than the copies from the preflight (40) and post flight (44) phases. In contrast, the control subjects shed EBV DNA with a frequency of 3.7% and mean EBV copies of 40 per mL of saliva. Ten days before flight and on landing day, titers of antibody to EBV viral capsid antigen were significantly (p < 0.05) greater than baseline levels. Increases in the number of viral copies and in the amount of EBV-specific antibody were consistent with EBV reactivation before, during, and after space flight. Similarly, CMV and VZV reactivation increased in response to space flight conditions. Data indicates that space flight is a unique stress environment that may produce stress-induced changes in the host-microbe relationship resulting in increased risk of infection.

Sleep and circadian rhythms in four orbiting astronauts.

PubMed

Monk, T H; Buysse, D J; Billy, B D; Kennedy, K S; Willrich, L M

1998-06-01

This experiment measured the sleep and circadian rhythms of four male astronauts aboard a space shuttle (STS-78) orbiting the Earth for 17 days. The space mission was specially scheduled to minimize disruptions in circadian rhythms and sleep so that the effects of space flight and microgravity per se could be studied. Data were collected in 72-h measurement blocks: one block 7 days before launch, one early within the mission (3 days after launch), one late in the mission (12 days after launch), and one 18 days after landing. Within each measurement block, all sleep was recorded both polysomnographically and by sleep diary. Core body temperature was sampled every 6 mins. Actillumes were worn continuously. All urine samples were collected separately. Performance was assessed by a computerized test battery (3/day) and by end-of-shift questionnaires (1/day); mood and alertness were measured by visual analogue scales (5/day). Circadian rhythms in orbit appeared to be very similar in phase and amplitude to those on the ground, and were appropriately aligned for the required work/rest schedule. There was no change from early flight to late flight. This was also reflected in mood, alertness, and performance scores, which were satisfactory at both in-flight time points. However, in-flight sleep showed a decreased amount of sleep obtained (mean = 6.1 h), and all four astronauts showed a decrease in delta sleep. No further degradation in sleep was seen when early flight was compared to late flight, and no other sleep parameters showed reliable trends.

Description of International Caenorhabditis elegans Experiment first flight (ICE-FIRST)

PubMed Central

Szewczyk, N.J.; Tillman, J.; Conley, C.A.; Granger, L.; Segalat, L.; Higashitani, A.; Honda, S.; Honda, Y.; Kagawa, H.; Adachi, R.; Higashibata, A.; Fujimoto, N.; Kuriyama, K.; Ishioka, N.; Fukui, K.; Baillie, D.; Rose, A.; Gasset, G.; Eche, B.; Chaput, D.; Viso, M.

2008-01-01

Traveling, living and working in space is now a reality. The number of people and length of time in space is increasing. With new horizons for exploration it becomes more important to fully understand and provide countermeasures to the effects of the space environment on the human body. In addition, space provides a unique laboratory to study how life and physiologic functions adapt from the cellular level to that of the entire organism. Caenorhabditis elegans is a genetic model organism used to study physiology on Earth. Here we provide a description of the rationale, design, methods, and space culture validation of the ICE-FIRST payload, which engaged C. elegans researchers from four nations. Here we also show C. elegans growth and development proceeds essentially normally in a chemically defined liquid medium on board the International Space Station (10.9 day round trip). By setting flight constraints first and bringing together established C. elegans researchers second, we were able to use minimal stowage space to successfully return a total of 53 independent samples, each containing more than a hundred individual animals, to investigators within one year of experiment concept. We believe that in the future, bringing together individuals with knowledge of flight experiment operations, flight hardware, space biology, and genetic model organisms should yield similarly successful payloads. PMID:22146801

Description of International Caenorhabditis elegans Experiment first flight (ICE-FIRST)

NASA Astrophysics Data System (ADS)

Szewczyk, N. J.; Tillman, J.; Conley, C. A.; Granger, L.; Segalat, L.; Higashitani, A.; Honda, S.; Honda, Y.; Kagawa, H.; Adachi, R.; Higashibata, A.; Fujimoto, N.; Kuriyama, K.; Ishioka, N.; Fukui, K.; Baillie, D.; Rose, A.; Gasset, G.; Eche, B.; Chaput, D.; Viso, M.

2008-09-01

Traveling, living and working in space is now a reality. The number of people and length of time in space is increasing. With new horizons for exploration it becomes more important to fully understand and provide countermeasures to the effects of the space environment on the human body. In addition, space provides a unique laboratory to study how life and physiologic functions adapt from the cellular level to that of the entire organism. Caenorhabditis elegans is a genetic model organism used to study physiology on Earth. Here we provide a description of the rationale, design, methods, and space culture validation of the ICE-FIRST payload, which engaged C. elegans researchers from four nations. Here we also show C. elegans growth and development proceeds essentially normally in a chemically defined liquid medium on board the International Space Station (10.9 day round trip). By setting flight constraints first and bringing together established C. elegans researchers second, we were able to use minimal stowage space to successfully return a total of 53 independent samples, each containing more than a hundred individual animals, to investigators within one year of experiment concept. We believe that in the future, bringing together individuals with knowledge of flight experiment operations, flight hardware, space biology, and genetic model organisms should yield similarly successful payloads.

Description of International Caenorhabditis elegans Experiment first flight (ICE-FIRST).

PubMed

Szewczyk, N J; Tillman, J; Conley, C A; Granger, L; Segalat, L; Higashitani, A; Honda, S; Honda, Y; Kagawa, H; Adachi, R; Higashibata, A; Fujimoto, N; Kuriyama, K; Ishioka, N; Fukui, K; Baillie, D; Rose, A; Gasset, G; Eche, B; Chaput, D; Viso, M

2008-09-15

Traveling, living and working in space is now a reality. The number of people and length of time in space is increasing. With new horizons for exploration it becomes more important to fully understand and provide countermeasures to the effects of the space environment on the human body. In addition, space provides a unique laboratory to study how life and physiologic functions adapt from the cellular level to that of the entire organism. Caenorhabditis elegans is a genetic model organism used to study physiology on Earth. Here we provide a description of the rationale, design, methods, and space culture validation of the ICE-FIRST payload, which engaged C. elegans researchers from four nations. Here we also show C. elegans growth and development proceeds essentially normally in a chemically defined liquid medium on board the International Space Station (10.9 day round trip). By setting flight constraints first and bringing together established C. elegans researchers second, we were able to use minimal stowage space to successfully return a total of 53 independent samples, each containing more than a hundred individual animals, to investigators within one year of experiment concept. We believe that in the future, bringing together individuals with knowledge of flight experiment operations, flight hardware, space biology, and genetic model organisms should yield similarly successful payloads.

Immune Modulation in Normal Human Peripheral Blood Mononuclear Cells (PBMCs) (Lymphocytes) in Response to Benzofuran-2-Carboxylic Acid Derivative KMEG during Spaceflight

NASA Astrophysics Data System (ADS)

Okoro, Elvis; Mann, Vivek; Ellis, Ivory; Mansoor, Elvedina; Olamigoke, Loretta; Marriott, Karla Sue; Denkins, Pamela; Williams, Willie; Sundaresan, Alamelu

2017-08-01

Microgravity and radiation exposure during space flight have been widely reported to induce the suppression of normal immune system function, and increase the risk of cancer development in humans. These findings pose a serious risk to manned space missions. Interestingly, recent studies have shown that benzofuran-2-carboxylic acid derivatives can inhibit the progression of some of these devastating effects on earth and in modeled microgravity. However, these studies had not assessed the impacts of benzofuran-2- carboxylic acid and its derivatives on global gene expression under spaceflight conditions. In this study, the ability of a specific benzofuran-2-carboxylic acid derivative (KMEG) to confer protection from radiation and restore normal immune function was investigated following exposure to space flight conditions on the ISS. Normal human peripheral blood mononuclear cells (lymphocytes) treated with 10 µ g/ml of KMEG together with untreated control samples were flown on Nanoracks hardware on Spacex-3 flight. The Samples were returned one month later and gene expression was analyzed. A 1g-ground control experiment was performed in parallel at the Kennedy spaceflight center. The first overall subtractive unrestricted analysis revealed 78 genes, which were differentially expressed in space flight KMEG, untreated lymphocytes as compared to the corresponding ground controls. However, in KMEG-treated space flight lymphocytes, there was an increased expression of a group of genes that mediate increased transcription, translation and innate immune system mediating functions of lymphocytes as compared to KMEG-untreated samples. Analysis of genes related to T cell proliferation in spaceflight KMEG-treated lymphocytes compared to 1g-ground KMEG- treated lymphocytes revealed six T cell proliferation and signaling genes to be significantly upregulated (p < 0.001) and five related genes were found to be significantly down-regulated. These genes play a significant role in promoting the proliferation of T-lymphocytes, the regulation of membrane trafficking, promote early response, mediating C-myc related proliferation, promote antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Overall, our analysis indicates that KMEG promotes T- cell proliferation and has an anti-inflammatory effect, thereby increasing immunity and possible protection from chronic inflammation setting which is optimally required during long term space flights.

An Update on Mortality in the U.S. Astronaut Corps: 1959-2009

NASA Technical Reports Server (NTRS)

Amirian, E.; Clark, April; Halm, Melissa; Hartnett, Heather

2009-01-01

Although it has now been over 50 years since mankind first ventured into space, the long-term health impacts of human space flight remain largely unknown. Identifying factors that affect survival and prognosis among those who participate in space flight is vitally important, as the era of commercial space flight approaches and NASA prepares for missions to Mars. The Longitudinal Study of Astronaut Health is a prospective study designed to examine trends in astronaut morbidity and mortality. The purpose of this analysis was to describe and explore predictors of overall and cause-specific mortality among individuals selected for the U.S. astronaut corps. All U.S. astronauts (n=321), regardless of flight status, were included in this analysis. Death certificate searches were conducted to ascertain vital status and cause of death through April 2009. Data were collected from medical records and lifestyle questionnaires. Multivariable Cox regression modeling was used to calculate the mortality hazard associated with embarking on space flight, adjusted for sex, race, and age at selection. Between 1959 and 2009, there were 39 (12.1%) deaths. Of these deaths, 18 (42.2%) were due to occupational accidents; 7 (17.9%) were due to other accidents; 6 (15.4%) were attributable to cancer; 6 (15.4%) resulted from cardiovascular/circulatory diseases; and 2 (5.1%) were from other causes. Participation in space flight did not significantly increase mortality hazard over time (adjusted hazard ratio=0.57; 95% confidence interval=0.26-1.26. Because our results are based on a small sample size, future research that includes payload specialists, other space flight participants, and international crew members is warranted to maximize statistical power.

Renal-Stone Risk Assessment During Space Shuttle Flights

NASA Technical Reports Server (NTRS)

Whitson, Peggy A.; Pietrzyk, Robert A.; Pak, Charles Y. C.

1996-01-01

The metabolic and environmental factors influencing renal stone formation before, during, and after Space Shuttle flights were assessed. We established the contributing roles of dietary factors in relationship to the urinary risk factors associated with renal stone formation. 24-hr urine samples were collected prior to, during space flight, and following landing. Urinary factors associated with renal stone formation were analyzed and the relative urinary supersaturation ratios of calcium oxalate, calcium phosphate (brushite), sodium urate, struvite and uric acid were calculated. Food and fluid consumption was recorded for a 48-hr period ending with the urine collection. Urinary composition changed during flight to favor the crystallization of stone-forming salts. Factors that contributed to increased potential for stone formation during space flight were significant reductions in urinary pH and increases in urinary calcium. Urinary output and citrate, a potent inhibitor of calcium-containing stones, were slightly reduced during space flight. Dietary intakes were significantly reduced for a number of variables, including fluid, energy, protein, potassium, phosphorus and magnesium. This is the first in-flight characterization of the renal stone forming potential in astronauts. With the examination of urinary components and nutritional factors, it was possible to determine the factors that contributed to increased risk or protected from risk. In spite of the protective components, the negative contributions to renal stone risk predominated and resulted in a urinary environment that favored the supersaturation of stone-forming salts. The importance of the hypercalciuria was noted since renal excretion was high relative to the intake.

Iron status and its relations with oxidative damage and bone loss during long-duration space flight on the International Space Station.

PubMed

Zwart, Sara R; Morgan, Jennifer L L; Smith, Scott M

2013-07-01

Increases in stored iron and dietary intake of iron during space flight have raised concern about the risk of excess iron and oxidative damage, particularly in bone. The objectives of this study were to perform a comprehensive assessment of iron status in men and women before, during, and after long-duration space flight and to quantify the association of iron status with oxidative damage and bone loss. Fasting blood and 24-h urine samples were collected from 23 crew members before, during, and after missions lasting 50 to 247 d to the International Space Station. Serum ferritin and body iron increased early in flight, and transferrin and transferrin receptors decreased later, which indicated that early increases in body iron stores occurred through the mobilization of iron to storage tissues. Acute phase proteins indicated no evidence of an inflammatory response during flight. Serum ferritin was positively correlated with the oxidative damage markers 8-hydroxy-2'-deoxyguanosine (r = 0.53, P < 0.001) and prostaglandin F2α (r = 0.26, P < 0.001), and the greater the area under the curve for ferritin during flight, the greater the decrease in bone mineral density in the total hip (P = 0.031), trochanter (P = 0.006), hip neck (P = 0.044), and pelvis (P = 0.049) after flight. Increased iron stores may be a risk factor for oxidative damage and bone resorption.

Kotov with Cryogem-03 refrigerator in the Zvezda Service module

NASA Image and Video Library

2007-04-01

ISS014-E-19179 (April 2007) --- Cosmonaut Oleg V. Kotov, Expedition 15 flight engineer representing Russia's Federal Space Agency, works with a test sample in the Zvezda Service Module of the International Space Station.

Infrared On-Orbit RCC Inspection With the EVA IR Camera: Development of Flight Hardware From a COTS System

NASA Technical Reports Server (NTRS)

Gazanik, Michael; Johnson, Dave; Kist, Ed; Novak, Frank; Antill, Charles; Haakenson, David; Howell, Patricia; Jenkins, Rusty; Yates, Rusty; Stephan, Ryan;

Activity of the sympathoadrenal system in cosmonauts during 25-day space flight on station Mir

NASA Astrophysics Data System (ADS)

Kvetňanský, R.; Noskov, V. B.; Blazicek, P.; Gharib, C.; Popova, I. A.; Gauquelin, G.; Macho, L.; Guell, A.; Grigoriev, A. I.

The activity of the sympathoadrenal system in cosmonauts was studied by measuring plasma and urinary catecholamines and their metabolites and conjugates. The appliance Plasma 02 was used for collecting, processing, and storing blood and urine samples from the cosmonauts during the course of a 25-day flight on board the station Mir. Plasma and urine concentrations of adrenaline (A), noradrenaline (NA), and dopamine (DA) as well as urinary levels of vanillylmandelic acid (VMA) and homovanillic acid (HVA), and plasma levels of catecholamine sulphates were determined before, during and after the space flight. Plasma NA levels were slightly elevated on day 9 and plasma A on day 20, whereas plasma DA levels were unchanged. However, most of the changes were within the normal range of control values. Sulphates of plasma catecholamines did not change during flight but they were significantly elevated after landing. Urinary levels of A, NA, DA, VMA, and HVA were comparable with preflight values but were elevated at the different intervals studied after landing. The results obtained suggest that in the short period of about 9 days of the cosmonaut's stay in space the sympathoadrenal system was slightly activated indicating a mild stressful influence of the initial period of flight. This short-term space flight compared to long-term flight did not as markedly activate the sympathoadrenal system during the process of re-adaptation to Earth's gravity after landing. Our data suggest that weightlessness is not a stressful factor activating the sympathoadrenal system but it sensitizes the responsiveness of this system during the re-adaptation period after space flight.

NASA Global Atmospheric Sampling Program (GASP) data report for tapes VL0011 and VL0013

NASA Technical Reports Server (NTRS)

Holdeman, J. D.; Dudzinski, T. J.; Tiefermann, M. W.

1979-01-01

In-situ measurements of atmospheric ozone, carbon monoxide, clouds, and related meteorological and flight information obtained during 1122 flights of aircraft VH-EBE and N655PA from January 10 through October 2, 1977 are reported. In addition, tropopause pressures obtained from time and space interpolation of achieved data for the dates of the flights are included.

The MISSE 7 Flexural Stress Effects Experiment After 1.5 Years of Wake Space Exposure

NASA Technical Reports Server (NTRS)

Snow, Kate E.; De Groh, Kim K.; Banks, Bruce A.

2017-01-01

Low Earth orbit space environment conditions, including ultraviolet radiation, thermal cycling, and atomic oxygen exposure, can cause degradation of exterior spacecraft materials over time. Radiation and thermal exposure often results in bond- breaking and embrittlement of polymers, reducing mechanical strength and structural integrity. An experiment called the Flexural Stress Effects Experiment (FSEE) was flown with the objective of determining the role of space environmental exposure on the degradation of polymers under flexural stress. The FSEE samples were flown in the wake orientation on the exterior of International Space Station for 1.5 years. Twenty-four samples were flown: 12 bent over a 0.375 in. mandrel and 12 were over a 0.25 in. mandrel. This was designed to simulate flight configurations of insulation blankets on spacecraft. The samples consisted of assorted polyimide and fluorinated polymers with various coatings. Half the samples were designated for bend testing and the other half will be tensile tested. A non-standard bend-test procedure was designed to determine the surface strain at which embrittled polymers crack. All ten samples designated for bend testing have been tested. None of the control samples' polymers cracked, even under surface strains up to 19.7%, although one coating cracked. Of the ten flight samples tested, seven show increased embrittlement through bend-test induced cracking at surface strains from 0.70%to 11.73%. These results show that most of the tested polymers are embrittled due to space exposure, when compared to their control samples. Determination of the extent of space induced embrittlement of polymers is important for designing durable spacecraft.

Nutritional Status Assessment (SMO 016E)

NASA Technical Reports Server (NTRS)

Smith, S. M.; Zwart, S. R.; Heer, M.; Ericson, K.; Coburn, S. P.; Booth, S. A.; Jones, J. A.; Lupton, J.

2009-01-01

Until 2006, it was not been possible to assess nutritional status of crewmembers on the ISS during flight because blood and urine could not be collected during ISS missions. Postflight observations of alterations in status of several nutrients are troubling, and we require the ability to monitor the status of these nutrients during flight to determine if there is a specific impetus or timeframe for these changes. In addition to the monitoring of crew nutritional status during flight, in-flight sample collection would allow better assessment of countermeasure effectiveness. Collecting samples during flight is one of the objectives of SMO 016E, and it is also designed to expand the current medical requirement for nutritional assessment (MR016L) to include additional normative markers for assessing crew health and countermeasure effectiveness. Additional markers of bone metabolism will be measured to better monitor bone health and the effectiveness of countermeasures to prevent bone resorption. New markers of oxidative damage will be measured to better assess the type of oxidative insults that occur during space flight. The array of nutritional assessment variables will be expanded to include ones that will allow us to better understand changes in folate, vitamin K, and vitamin B6 status, as well as risk factors for cardiovascular and oxidative damage during and after flight. Stress hormones and hormones that affect bone and muscle metabolism will also be measured. Measuring these additional variables will allow us to better monitor the health of crewmembers and make more accurate recommendations for their rehabilitation. Several nutritional assessment variables are altered at landing, but it is not known how long these changes persist. We extended the original protocol to include an additional postflight blood and urine sample collection 30 days after landing. Data are being collected before, during, and after flight. These data will provide a complete survey of how nutritional status and related systems are affected by space flight. Analyzing the data will help us to define nutritional requirements for long-duration missions. This expanded set of measurements will also aid in the identification of nutritional countermeasures to counteract, for example, the deleterious effects of microgravity on bone and muscle and the effects of space radiation.

Wiseman in with ACE sample

NASA Image and Video Library

2014-05-30

ISS040-E-006569 (2 June 2014) --- NASA astronaut Reid Wiseman, Expedition 40 flight engineer, performs an Advanced Colloids Experiment (ACE) sample 40-minute mixing activity in the Destiny laboratory of the International Space Station.

Wiseman in with ACE sample

NASA Image and Video Library

2014-05-30

ISS040-E-006567 (2 June 2014) --- NASA astronaut Reid Wiseman, Expedition 40 flight engineer, performs an Advanced Colloids Experiment (ACE) sample 40-minute mixing activity in the Destiny laboratory of the International Space Station.

Validation of Procedures for Monitoring Crewmember Immune Function

NASA Technical Reports Server (NTRS)

Crucian, Brian; Stowe, Raymond; Mehta, Satish; Uchakin, Peter; Quiriarte, Heather; Pierson, Duane; Sams, Clarence

2009-01-01

There is ample evidence to suggest that space flight leads to immune system dysregulation, however the nature of the phenomenon as it equilibrates over longer flights has not been determined. This dysregulation may be a result of microgravity, confinement, physiological stress, radiation, environment or other mission-associated factors. The clinical risk (if any) for exploration-class space flight is unknown, but may include increased incidence of infection, allergy, hypersensitivity, hematological malignancy or altered wound healing. The objective of this Supplemental Medical Objective (SMO) is to determine the status of the immune system, physiological stress and latent viral reactivation (a clinical outcome that can be measured) during both short and long-duration spaceflight. In addition, this study will develop and validate an immune monitoring strategy consistent with operational flight requirements and constraints. Pre-mission, in-flight and post-flight blood and saliva samples will be obtained from participating crewmembers. Assays included peripheral immunophenotype, T cell function, cytokine profiles (RNA, intracellular, secreted), viral-specific immunity, latent viral reactivation (EBV, CMV, VZV), and stress hormone measurements. This study is currently ongoing. To date, 10 short duration and 5 long-duration crewmembers have completed the study. Technically, the study is progressing well. In-flight blood samples are being collected, and returned for analysis, including functional assays that require live cells. For all in-flight samples to date, sample viability has been acceptable. Preliminary data (n = 4/7; long/short duration, respectively) indicate that distribution of most peripheral leukocyte subsets is largely unaltered during flight. Exceptions include elevated T cells, reduced B/NK cells, increased memory T cells and increased central memory CD8+ T cells. General T cell function, early blastogenesis response to mitogenic stimulation, is markedly reduced in-flight. In-vivo cytokine production profiles are altered, with in-flight dysregulation observed in the Th1/Th2/Treg equilibrium. EBV specific T cell levels are increased during flight, whereas their function is reduced. VZV reactivation was observed inflight and several days post flight with highest levels measured later during long-duration flight. The shedding of CMV in the urine was detected of 4/5 long duration and 4/7 short duration crewmembers. Plasma cortisol was not elevated during flight. Further data will be required to validate the initial observations.

Air and Space Museum Apollo 40th Celebration

NASA Image and Video Library

2009-07-19

NASA Astronaut and Expedition 19 Flight Engineer Michael Barratt delivers remarks and shows a moon rock sample being flown onboard the International Space Station at the Apollo 40th anniversary celebration held at the National Air and Space Museum, Monday, July 20, 2009 in Washington. Photo Credit: (NASA/Bill Ingalls)

Reactivation of latent herpes viruses in cosmonauts during a soyuz taxi mission

NASA Astrophysics Data System (ADS)

Mehta, Satish K.; Pierson, Duane L.

2007-09-01

The hypothesis tested by this project is that space flight increases the incidence and duration of herpes virus reactivation and shedding in saliva. Saliva, urine, and blood samples were collected from 3 crew members who participated in a 14-day Odessa Soyuz taxi mission. Saliva samples were collected before, during, and after the mission, and blood and urine were collected before and after the mission. The saliva and urine samples were analyzed using the polymerase chain reaction to detect the presence of 3 important herpes viruses. Epstein-Barr virus (EBV) and varicella-zoster virus (VZV) were tested in saliva, and cytomegalovirus (CMV) was measured in urine samples. Plasma antibodies levels to these viruses were determined by enzyme-linked immunosorbent assay before and after flight. EBV reactivated before, during, and after flight; CMV reactivated before and after flight; and VZV reactivated during and after flight. In other studies, greater frequencies of positive samples and greater numbers of copies of viral DNA have been found. No increases in titer of antibodies to these viruses were found, suggesting that an immune response may not be necessary for reactivation.

Urinary Acid Excretion Can Predict Changes in Bone Metabolism During Space Flight

NASA Technical Reports Server (NTRS)

Zwart, Sara R.; Smith, Scott M.

2011-01-01

Mitigating space flight-induced bone loss is critical for space exploration, and a dietary countermeasure would be ideal. We present here preliminary data from a study where we examined the role of dietary intake patterns as one factor that can influence bone mineral loss in astronauts during space flight. Crewmembers (n=5) were asked to consume a prescribed diet with either a low (0.3-0.6) or high (1.0-1.3) ratio of animal protein to potassium (APro:K) before and during space flight for 4-d periods. Diets were controlled for energy, total protein, calcium, and sodium. 24-h urine samples were collected on the last day of each of the 4-d controlled diet sessions. 24-h urinary acid excretion, which was predicted by dietary potential renal acid load, was correlated with urinary n-telopeptide (NTX, Pearson R = 0.99 and 0.80 for the high and low APro:K sessions, respectively, p<0.001). The amount of protein when expressed as the percentage of total energy (but not as total grams) was also correlated with urinary NTX (R = 0.66, p<0.01). These results, from healthy individuals in a unique environment, will be important to better understand diet and bone interrelationships during space flight as well as on Earth. The study was funded by the NASA Human Research Program.

Effect of Sampling Schedule on Pharmacokinetic Parameter Estimates of Promethazine in Astronauts

NASA Technical Reports Server (NTRS)

Boyd, Jason L.; Wang, Zuwei; Putcha, Lakshmi

2005-01-01

Six astronauts on the Shuttle Transport System (STS) participated in an investigation on the pharmacokinetics of promethazine (PMZ), a medication used for the treatment of space motion sickness (SMS) during flight. Each crewmember completed the protocol once during flight and repeated thirty days after returned to Earth. Saliva samples were collected at scheduled times for 72 h after PMZ administration; more frequent samples were collected on the ground than during flight owing to schedule constraints in flight. PMZ concentrations in saliva were determined by a liquid chromatographic/mass spectrometric (LC-MS) assay and pharmacokinetic parameters (PKPs) were calculated using actual flight and ground-based data sets and using time-matched sampling schedule on ground to that during flight. Volume of distribution (V(sub c)) and clearance (Cl(sub s),) decreased during flight compared to that from time-matched ground data set; however, Cl(sub s) and V(sub c) estimates were higher for all subjects when partial ground data sets were used for analysis. Area under the curve (AUC) normalized with administered dose was similar in flight and partial ground data; however AUC was significantly lower using time-matched sampling compared with the full data set on ground. Half life (t(sub 1/2)) was longest during flight, shorter with matched-sampling schedule on ground and shortest when complete data set from ground was used. Maximum concentration (C(sub max)), time for C(sub max), (t(sub max)), parameters of drug absorption, depicted a similar trend with lowest and longest respectively, during flight, lower with time-matched ground data and highest and shortest with full ground data.

Effect of sampling schedule on pharmacokinetic parameter estimates of promethazine in astronauts

NASA Astrophysics Data System (ADS)

Boyd, Jason L.; Wang, Zuwei; Putcha, Lakshmi

2005-08-01

Six astronauts on the Shuttle Transport System (STS) participated in an investigation on the pharmacokinetics of promethazine (PMZ), a medication used for the treatment of space motion sickness (SMS) during flight. Each crewmember completed the protocol once during flight and repeated thirty days after returned to Earth. Saliva samples were collected at scheduled times for 72 h after PMZ administration; more frequent samples were collected on the ground than during flight owing to schedule constraints in flight. PMZ concentrations in saliva were determined by a liquid chromatographic/mass spectrometric (LC-MS) assay and pharmacokinetic parameters (PKPs) were calculated using actual flight and ground-based data sets and using time-matched sampling schedule on ground to that during flight. Volume of distribution (Vc) and clearance (Cls) decreased during flight compared to that from time-matched ground data set; however, ClS and Vc estimates were higher for all subjects when partial ground data sets were used for analysis. Area under the curve (AUC) normalized with administered dose was similar in flight and partial ground data; however AUC was significantly lower using time-matched sampling compared with the full data set on ground. Half life (t1/2) was longest during flight, shorter with matched-sampling schedule on ground and shortest when complete data set from ground was used. Maximum concentration (Cmax), time for Cmax (tmax), parameters of drug absorption, depicted a similar trend with lowest and longest respectively, during flight, lower with time- matched ground data and highest and shortest with full ground data.

Nutritional Status Assessment (SMO -16E)

NASA Technical Reports Server (NTRS)

Smith, Scott M.; Heer, M. A.; Zwart, S. R.

2012-01-01

The Nutritional Status Assessment Supplemental Medical Objective was an experiment initiated to expand nominal pre- and postflight clinical nutrition testing, and to gain a better understanding of the time course of changes during flight. The primary activity of this effort was collecting blood and urine samples 5 times during flight for analysis after return to Earth. Samples were subjected to a battery of tests, including nutritional, physiological, general chemistry, and endocrinology indices. These data provide a comprehensive survey of how nutritional status and related systems are affected by 4-6 months of space flight. Analyzing the data will help us to define nutritional requirements for long-duration missions, and better understand human adaptation to microgravity. This expanded set of measurements will also aid in the identification of nutritional countermeasures to counteract, for example, the deleterious effects of microgravity on bone and muscle and the effects of space radiation.

The Student Spaceflight Experiments Program: Access to the ISS for K-14 Students

NASA Astrophysics Data System (ADS)

Livengood, Timothy A.; Goldstein, J. J.; Vanhala, H. A. T.; Johnson, M.; Hulslander, M.

2012-10-01

The Student Spaceflight Experiments Program (SSEP) has flown 42 experiments to space, on behalf of students from middle school through community college, on 3 missions: each of the last 2 Space Shuttle flights, and the first SpaceX resupply flight to the International Space Station (ISS). SSEP plans 2 missions to the ISS per year for the foreseeable future, and is expanding the program to include 4-year undergraduate college students and home-schooled students. SSEP experiments have explored biological, chemical, and physical phenomena within self-contained enclosures developed by NanoRacks, currently in the form of MixStix Fluid Mixing Enclosures. Over 9000 students participated in the initial 3 missions of SSEP, directly experiencing the entire lifecycle of space science experimentation through community-wide participation in SSEP, taking research from a nascent idea through developing competitive research proposals, down-selecting to three proposals from each participating community and further selection of a single proposal for flight, actual space flight, sample recovery, analysis, and reporting. The National Air and Space Museum has hosted 2 National Conferences for SSEP student teams to report results in keeping with the model of professional research. Student teams have unflinchingly reported on success, failure, and groundbased efforts to develop proposals for future flight opportunities. Community participation extends outside the sciences and the immediate proposal efforts to include design competitions for mission patches (that also fly to space). Student experimenters have rallied around successful proposal teams to support a successful experiment on behalf of the entire community. SSEP is a project of the National Center for Earth and Space Science Education enabled through NanoRacks LLC, working in partnership with NASA under a Space Act Agreement as part of the utilization of the International Space Station as a National Laboratory.

Planetary and Space Simulation Facilities (PSI) at DLR

NASA Astrophysics Data System (ADS)

Panitz, Corinna; Rabbow, E.; Rettberg, P.; Kloss, M.; Reitz, G.; Horneck, G.

2010-05-01

The Planetary and Space Simulation facilities at DLR offer the possibility to expose biological and physical samples individually or integrated into space hardware to defined and controlled space conditions like ultra high vacuum, low temperature and extraterrestrial UV radiation. An x-ray facility stands for the simulation of the ionizing component at the disposal. All of the simulation facilities are required for the preparation of space experiments: - for testing of the newly developed space hardware - for investigating the effect of different space parameters on biological systems as a preparation for the flight experiment - for performing the 'Experiment Verification Tests' (EVT) for the specification of the test parameters - and 'Experiment Sequence Tests' (EST) by simulating sample assemblies, exposure to selected space parameters, and sample disassembly. To test the compatibility of the different biological and chemical systems and their adaptation to the opportunities and constraints of space conditions a profound ground support program has been developed among many others for the ESA facilities of the ongoing missions EXPOSE-R and EXPOSE-E on board of the International Space Station ISS . Several experiment verification tests EVTs and an experiment sequence test EST have been conducted in the carefully equipped and monitored planetary and space simulation facilities PSI of the Institute of Aerospace Medicine at DLR in Cologne, Germany. These ground based pre-flight studies allowed the investigation of a much wider variety of samples and the selection of the most promising organisms for the flight experiment. EXPOSE-E had been attached to the outer balcony of the European Columbus module of the ISS in February 2008 and stayed for 1,5 years in space; EXPOSE-R has been attached to the Russian Svezda module of the ISS in spring 2009 and mission duration will be approx. 1,5 years. The missions will give new insights into the survivability of terrestrial organisms in space and will contribute to the understanding of the organic chemistry processes in space, the biological adaptation strategies to extreme conditions, e.g. on early Earth and Mars, and the distribution of life beyond its planet of origin The results gained during the simulation experiments demonstrated mission preparation as a basic requirement for successful and significant results of every space flight experiment. Hence, the Mission preparation program that was performed in the context of the space missions EXPOSE-E and EXPOSE-R proofed the outstanding importance and accentuated need for ground based experiments before and during a space mission. The facilities are also necessary for the performance of the ground control experiment during the mission, the so-called Mission Simulation Test (MST) under simulated space conditions, by parallel exposure of samples to simulated space parameters according to flight data received by telemetry. Finally the facilities also provide the possibility to simulate the surface and climate conditions of the planet Mars. In this way they offer the possibility to investigate under simulated Mars conditions the chances for development of life on Mars and to gain previous knowledge for the search for life on today's Mars and in this context especially the parameters for a manned mission to Mars. References [1] Rabbow E, Rettberg P, Panitz C, Drescher J, Horneck G, Reitz G (2005) SSIOUX - Space Simulation for Investigating Organics, Evolution and Exobiology, Adv. Space Res. 36 (2) 297-302, doi:10.1016/j.asr.2005.08.040Aman, A. and Bman, B. (1997) JGR, 90,1151-1154. [2] Fekete A, Modos K, Hegedüs M, Kovacs G, Ronto Gy, Peter A, Lammer H, Panitz C (2005) DNA Damage under simulated extraterrestrial conditions in bacteriophage T7 Adv. Space Res. 305-310Aman, A. et al. (1997) Meteoritics & Planet. Sci., 32,A74. [3] Cockell Ch, Schuerger AC, Billi D., Friedmann EI, Panitz C (2005) Effects of a Simulated Martian UV Flux on the Cyanobacterium, Chroococcidiopsis sp. 029, Astrobiology, 5/2 127-140Aman, A. (1996) LPS XXVII, 1344-1 [4] de la Torre Noetzel, R.; Sancho, L.G.; Pintado,A.; Rettberg, Petra; Rabbow, Elke; Panitz,Corinna; Deutschmann, U.; Reina, M.; Horneck, Gerda (2007): BIOPAN experiment LICHENS on the Foton M2 mission Pre-flight verification tests of the Rhizocarpon geographicum-granite ecosystem. COSPAR [Hrsg.]: Advances in Space Research, 40, Elsevier, S. 1665 - 1671, DOI 10.1016/j.asr.2007.02.022

Changes in erythropoietin levels during space flight or space flight simulation

NASA Technical Reports Server (NTRS)

Dunn, C. D. R.; Hen, J. P.

1980-01-01

Two hundred and seventy samples from 24 subjects involved in 3 bedrest studies and from 3 subjects involved in Spacelab Mission Development Test 3 were assayed for erythropoietin (Ep), in an in vitro fetal mouse liver cell assay, and for ferritin using a commercially available immunoradiometric assay kit. No trends or significant changes in serum Ep were observed. Serum ferritin concentrations tended to increases slightly during the 'missions', reflecting a redirection of iron from the suppressed erythron into iron stores.

Marshall Space Flight Center Black History Month Program

NASA Image and Video Library

2018-02-21

The Black History Month program was presented at Marshall Space Flight Center with guest speaker Lt. General Stayce Harris. General Harris is the Inspector General of the Air Force and she is the first African American female Lieutenant General in the American military. The topic of her presentation was "African Americans in Times of War". The presentation was followed by an ethnic food sampling. General Harris is pictured here with local area Air Force JROTC cadets who attended the program.

Microgravity

NASA Image and Video Library

1998-10-10

Breast tissue specimens in traditional sample dishes. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunourous tissues.

Rodent Habitat on ISS: Advances in Capability for Determining Spaceflight Effects on Mammalian Physiology

NASA Technical Reports Server (NTRS)

Globus, R. K.; Choi, S.; Gong, C.; Leveson-Gower, D.; Ronca, A.; Taylor, E.; Beegle, J.

2016-01-01

Rodent research is a valuable essential tool for advancing biomedical discoveries in life sciences on Earth and in space. The National Research Counsel's Decadal survey (1) emphasized the importance of expanding NASAs life sciences research to perform long duration, rodent experiments on the International Space Station (ISS). To accomplish this objective, new flight hardware, operations, and science capabilities were developed at NASA ARC to support commercial and government-sponsored research. The flight phases of two separate spaceflight missions (Rodent Research-1 and Rodent Research-2) have been completed and new capabilities are in development. The first flight experiments carrying 20 mice were launched on Sept 21, 2014 in an unmanned Dragon Capsule, SpaceX4; Rodent Research-1 was dedicated to achieving both NASA validation and CASIS science objectives, while Rodent Reesearch-2 extended the period on orbit to 60 days. Groundbased control groups (housed in flight hardware or standard cages) were maintained in environmental chambers at Kennedy Space Center. Crewmembers previously trained in animal handling transferred mice from the Transporter into Habitats under simultaneous veterinary supervision by video streaming and were deemed healthy. Health and behavior of all mice on the ISS was monitored by video feed on a daily basis, and post-flight quantitative analyses of behavior were performed. The 10 mice from RR-1 Validation (16wk old, female C57Bl6/J) ambulated freely and actively throughout the Habitat, relying heavily on their forelimbs for locomotion. The first on-orbit dissections of mice were performed successfully, and high quality RNA (RIN values>9) and liver enzyme activities were obtained, validating the quality of sample recovery. Post-flight sample analysis revealed that body weights of FLT animals did not differ from ground controls (GC) housed in the same hardware, or vivarium controls (VIV) housed in standard cages. Organ weights analyzed post-flight showed that there were no differences between FLT and GC groups in adrenal gland and spleen weights, whereas FLT thymus and liver weights exceeded those of GC. Minimal differences between the control groups (GC and VIV) were observed. In addition, Over 3,000 aliquots collected post-flight from the four groups of mice were deposited into the Ames Life Science Data Archives for the Biospecimen Sharing Program and Genelab project. New capabilities recently developed include DEXA scanning, grip strength tests and male mice. In conclusion, new capability for long duration rodent habitation of group-housed rodents was developed and includes in-flight sample collection, thus avoiding the complication of reentry. Results obtained to date reveal the possibility of striking differences between the effects of short duration vs. long duration spaceflight. This Rodent Research system enables achievement of both basic science and translational research objectives to advance human exploration of space.

Nutrient Considerations for Plants Grown Under Space Flight Conditions

NASA Technical Reports Server (NTRS)

Levine, Howard G.; Krikorian, Abraham D.

2006-01-01

We present here results on the analysis of 100 mL medium samples extracted from sterilized foam (Smithers-Oasis, Kent OH) used to support the growth of both dicotyledonous (Haplopappus gracilis, n=75) and monocotyledonous (Hemerocallis cv Autumn Blaze, n=25) aseptic plants in NASA's Plant Growth Unit (PGU) during the 5-day CHROMEX-01 Space Shuttle flight (March 1989, STS-29). At recovery, the medium remaining within each of the five floral foam blocks (for both the space flight and ground control experiments) was extracted under vacuum, filtered and subjected to elemental analyses. Concentration levels of some elements remained the same, while some decreased and others increased. A unique aspect of this experiment was that all plants were either aseptic tissue culture generated plantlets or sterile seedling clones, and the design of the PGU facilitated the maintenance of asepsis throughout the mission (confirmed by postflight microbial sampling). This permitted the elimination of microbial considerations in the interpretation of the data. The significance of these findings for growing plants in altered gravity environments are discussed.

Gerst with MSG during BASS session

NASA Image and Video Library

2014-06-13

European Space Agency astronaut Alexander Gerst,Expedition 40 flight engineer,works with samples and hardware for a combustion experiment known as the Burning and Suppression of Solids (BASS) in the Microgravity Science Glovebox (MSG) in the Destiny laboratory of the International Space Station.

Changes in gene expression and signal transduction in microgravity

NASA Technical Reports Server (NTRS)

Hughes-Fulford, M.

2001-01-01

Studies from space flights over the past three decades have demonstrated that basic physiological changes occur in humans during space flight. These changes include cephalic fluid shifts, loss of fluid and electrolytes, loss of muscle mass, space motion sickness, anemia, reduced immune response, and loss of calcium and mineralized bone. The cause of most of these manifestations is not known and until recently, the general approach was to investigate general systemic changes, not basic cellular responses to microgravity. This laboratory has recently studied gene growth and activation of normal osteoblasts (MC3T3-El) during spaceflight. Osteoblast cells were grown on glass coverslips and loaded in the Biorack plunger boxes. The osteoblasts were launched in a serum deprived state, activated in microgravity and collected in microgravity. The osteoblasts were examined for changes in gene expression and signal transduction. Approximately one day after growth activation significant changes were observed in gene expression in 0-G flight samples. Immediate early growth genes/growth factors cox-2, c-myc, bcl2, TGF beta1, bFGF and PCNA showed a significant diminished mRNA induction in microgravity FCS activated cells when compared to ground and 1-G flight controls. Cox-1 was not detected in any of the samples. There were no significant differences in the expression of reference gene mRNA between the ground, 0-G and 1-G samples. The data suggest that quiescent osteoblasts are slower to enter the cell cycle in microgravity and that the lack of gravity itself may be a significant factor in bone loss in spaceflight. Preliminary data from our STS 76 flight experiment support our hypothesis that a basic biological response occurs at the tissue, cellular, and molecular level in 0-G. Here we examine ground-based and space flown data to help us understand the mechanism of bone loss in microgravity.

The dynamics of blood biochemical parameters in cosmonauts during long-term space flights

NASA Astrophysics Data System (ADS)

Markin, Andrei; Strogonova, Lubov; Balashov, Oleg; Polyakov, Valery; Tigner, Timoty

Most of the previously obtained data on cosmonauts' metabolic state concerned certain stages of the postflight period. In this connection, all conclusions, as to metabolism peculiarities during the space flight, were to a large extent probabilistic. The purpose of this work was study of metabolism characteristics in cosmonauts directly during long-term space flights. In the capillary blood samples taken from a finger, by "Reflotron IV" biochemical analyzer, "Boehringer Mannheim" GmbH, Germany, adapted to weightlessness environments, the activity of GOT, GPT, CK, gamma-GT, total and pancreatic amylase, as well as concentration of hemoglobin, glucose, total bilirubin, uric acid, urea, creatinine, total, HDL- and LDL cholesterol, triglycerides had been determined. HDL/LDL-cholesterol ratio also was computed. The crewmembers of 6 main missions to the "Mir" orbital station, a total of 17 cosmonauts, were examined. Biochemical tests were carryed out 30-60 days before lounch, and in the flights different stages between the 25-th and the 423-rd days of flights. In cosmonauts during space flight had been found tendency to increase, in compare with basal level, GOT, GPT, total amylase activity, glucose and total cholesterol concentration, and tendency to decrease of CK activity, hemoglobin, HDL-cholesterol concentration, and HDL/LDL — cholesterol ratio. Some definite trends in variations of other determined biochemical parameters had not been found. The same trends of mentioned biochemical parameters alterations observed in majority of tested cosmonauts, allows to suppose existence of connection between noted metabolic alterations with influence of space flight conditions upon cosmonaut's body. Variations of other studied blood biochemical parameters depends on, probably, pure individual causes.

Medical Research System

NASA Technical Reports Server (NTRS)

1993-01-01

Based on Johnson Space Flight Center's development of a rotating bioreactor cell culture apparatus for Space Shuttle medical research, Johnson Space Flight Center engineers who worked on the original project formed a company called Synthecon, with the intention of commercializing the bioreactor technology. Synthecon grows three dimensional tissues in the bioreactor. These are superior to previous two-dimensional tissue samples in the study of human cell growth. A refined version of the Johnson Space Center technology, Synthecon's Rotary Cell Culture System includes a cell culture chamber that rotates around a horizontal axis. The cells establish an orbit that approximates free fall through the liquid medium in the chamber. The technology has significant applications for cancer research and treatment as well as AIDS research.

MISSE Results Used for RF Plasma Ground Testing-To-Space-Exposure Correlation for Coated Kapton

NASA Technical Reports Server (NTRS)

Miller, Sharon K. R.; Banks, Bruce A.; Tollis, Greg

2008-01-01

The ability to predict the durability of materials in the low Earth orbit (LEO) environment by exposing them in ground-based facilities is important because one can achieve test results sooner, expose more types of materials, and do it much more cost effectively than to test them in flight. However, flight experiments to determine the durability of groups or classes of materials that behave similarly are needed in order to provide correlations of how much time in ground-based facilities represents certain durations in LEO for the material type of interest. An experiment was designed and flown on the Materials International Space Station Experiment (MISSE) 2 (3.95 yr in LEO) and MISSE 4 (1.04 yr in LEO) in order to develop this type of correlation between ground-based RF plasma exposure and LEO exposure for coated Kapton. The experiment consisted of a sample of Kapton H (DuPont) polyimide coated with 1300 of silicon dioxide by Sheldahl, Inc. The samples were exposed to atomic oxygen in a radio frequency (RF) generated atomic oxygen plasma. Mass change was measured for the samples and then the same samples were exposed in flight on MISSE and the mass change was again recorded post-flight. After documentation, the samples were exposed again in the ground-based RF plasma in order to determine if the erosion would be the same as it had been in the same facility pre-flight which would indicate whether or not the sample had been damaged during flight and if the defects on the surface were those that were there preflight. The slopes of the mass change versus fluence plots were then used to develop a correlation factor that can be used to help predict the durability of coated Kapton in ground-based isotropic atomic oxygen plasma systems. This paper describes the experiment and presents the correlation factor results.

The Importance of Contamination Knowledge in Curation - Insights into Mars Sample Return

NASA Technical Reports Server (NTRS)

Harrington, A. D.; Calaway, M. J.; Regberg, A. B.; Mitchell, J. L.; Fries, M. D.; Zeigler, R. A.; McCubbin, F. M.

2018-01-01

The Astromaterials Acquisition and Curation Office at NASA Johnson Space Center (JSC), in Houston, TX (henceforth Curation Office) manages the curation of extraterrestrial samples returned by NASA missions and shared collections from international partners, preserving their integrity for future scientific study while providing the samples to the international community in a fair and unbiased way. The Curation Office also curates flight and non-flight reference materials and other materials from spacecraft assembly (e.g., lubricants, paints and gases) of sample return missions that would have the potential to cross-contaminate a present or future NASA astromaterials collection.

Bioculture System Validation

NASA Technical Reports Server (NTRS)

Sato, Kevin Y.

2012-01-01

The Bioculture System first flight will be to validate the performance of the hardware and its automated and manual operational capabilities in the space flight environment of the International Space Station. Biology, Engineering, and Operations tests will be conducted in the Bioculture System fully characterize its automated and manual functions to support cell culturing for short and long durations. No hypothesis-driven research will be conducted with biological sample, and the science leads have all provided their concurrence that none of the data they collect will be considered as proprietary and can be free distributed to the science community. The outcome of the validation flight will be to commission the hardware for use by the science community. This presentation will provide non-proprietary details about the Bioculture System and information about the activities for the first flight.

NASA's Rodent Research Project: Validation of Flight Hardware, Operations and Science Capabilities for Conducting Long Duration Experiments in Space

NASA Technical Reports Server (NTRS)

Choi, S. Y.; Beegle, J. E.; Wigley, C. L.; Pletcher, D.; Globus, R. K.

2015-01-01

Research using rodents is an essential tool for advancing biomedical research on Earth and in space. Rodent Research (RR)-1 was conducted to validate flight hardware, operations, and science capabilities that were developed at the NASA Ames Research Center. Twenty C57BL/6J adult female mice were launched on Sept 21, 2014 in a Dragon Capsule (SpaceX-4), then transferred to the ISS for a total time of 21-22 days (10 commercial mice) or 37 (10 validation mice). Tissues collected on-orbit were either rapidly frozen or preserved in RNA later at less than or equal to -80 C (n=2/group) until their return to Earth. Remaining carcasses were rapidly frozen for dissection post-flight. The three controls groups at Kennedy Space Center consisted of: Basal mice euthanized at the time of launch, Vivarium controls, housed in standard cages, and Ground Controls (GC), housed in flight hardware within an environmental chamber. FLT mice appeared more physically active on-orbit than GC, and behavior analysis are in progress. Upon return to Earth, there were no differences in body weights between FLT and GC at the end of the 37 days in space. RNA was of high quality (RIN greater than 8.5). Liver enzyme activity levels of FLT mice and all control mice were similar in magnitude to those of the samples that were optimally processed in the laboratory. Liver samples collected from the intact frozen FLT carcasses had RNA RIN of 7.27 +/- 0.52, which was lower than that of the samples processed on-orbit, but similar to those obtained from the control group intact carcasses. Nonetheless, the RNA samples from the intact carcasses were acceptable for the most demanding transcriptomic analyses. Adrenal glands, thymus and spleen (organs associated with stress response) showed no significant difference in weights between FLT and GC. Enzymatic activity was also not significantly different. Over 3,000 tissues collected from the four groups of mice have become available for the Biospecimen Sharing Program. Together, these validation flight findings demonstrate the capability to support long-duration RR on the ISS to achieve both basic science and biomedical objectives.

Chemical Analysis Results for Potable Water from ISS Expeditions 21 Through 25

NASA Technical Reports Server (NTRS)

Straub, John E., II; Plumlee, Debrah K.; Schultz, John R.; McCoy, J. Torin

2011-01-01

The Johnson Space Center Water and Food Analytical Laboratory (WAFAL) performed detailed ground-based analyses of archival water samples for verification of the chemical quality of the International Space Station (ISS) potable water supplies for Expeditions 21 through 25. Over a 14-month period the Space Shuttle visited the ISS on four occasions to complete construction and deliver supplies. The onboard supplies of potable water available for consumption by the Expeditions 21 to 25 crews consisted of Russian ground-supplied potable water, Russian potable water regenerated from humidity condensate, and US potable water recovered from urine distillate and condensate. Chemical archival water samples that were collected with U.S. hardware during Expeditions 21 to 25 were returned on Shuttle flights STS-129 (ULF3), STS-130 (20A), STS-131 (19A), and STS-132 (ULF4), as well as on Soyuz flights 19-23. This paper reports the analytical results for these returned potable water archival samples and their compliance with ISS water quality standards.

Cognitive Assessment During Long-Duration Space Flight

NASA Technical Reports Server (NTRS)

Seaton, Kimberly; Kane, R. L.; Sipes, Walter

2010-01-01

The Space Flight Cognitive Assessment Tool for Windows (WinSCAT) is a computer-based, self-administered battery of five cognitive assessment tests developed for medical operations at NASA's Johnson Space Center in Houston, Texas. WinSCAT is a medical requirement for U.S. long-duration astronauts and has been implemented with U.S. astronauts from one NASA/Mir mission (NASA-7 mission) and all expeditions to date on the International Space Station (ISS). Its purpose is to provide ISS crew surgeons with an objective clinical tool after an unexpected traumatic event, a medical condition, or the cumulative effects of space flight that could negatively affect an astronaut's cognitive status and threaten mission success. WinSCAT was recently updated to add network capability to support a 6-person crew on the station support computers. Additionally, WinSCAT Version 2.0.28 has increased difficulty of items in Mathematics, increased number of items in Match-to-Sample, incorporates a moving rather than a fixed baseline, and implements stricter interpretation rules. ISS performance data were assessed to compare initial to modified interpretation rules for detecting potential changes in cognitive functioning during space flight. WinSCAT tests are routinely taken monthly during an ISS mission. Performance data from these ISS missions do not indicate significant cognitive decrements due to microgravity/space flight alone but have shown decrements. Applying the newly derived rules to ISS data results in a number of off-nominal performances at various times during and after flight.. Correlation to actual events is needed, but possible explanations for off-nominal performances could include actual physical factors such as toxic exposure, medication effects, or fatigue; emotional factors including stress from the mission or life events; or failure to exert adequate effort on the tests.

ISS Potable Water Quality for Expeditions 26 through 30

NASA Technical Reports Server (NTRS)

Straub, John E., II; Plumlee, Debrah K.; Schultz, John R.; McCoy, J. Torin

2012-01-01

International Space Station (ISS) Expeditions 26-30 spanned a 16-month period beginning in November of 2010 wherein the final 3 flights of the Space Shuttle program finished ISS construction and delivered supplies to support the post-shuttle era of station operations. Expedition crews relied on several sources of potable water during this period, including water recovered from urine distillate and humidity condensate by the U.S. water processor, water regenerated from humidity condensate by the Russian water recovery system, and Russian ground-supplied potable water. Potable water samples collected during Expeditions 26-30 were returned on Shuttle flights STS-133 (ULF5), STS-134 (ULF6), and STS-135 (ULF7), as well as Soyuz flights 24-27. The chemical quality of the ISS potable water supplies continued to be verified by the Johnson Space Center s Water and Food Analytical Laboratory (WAFAL) via analyses of returned water samples. This paper presents the chemical analysis results for water samples returned from Expeditions 26-30 and discusses their compliance with ISS potable water standards. The presence or absence of dimethylsilanediol (DMSD) is specifically addressed, since DMSD was identified as the primary cause of the temporary rise and fall in total organic carbon of the U.S. product water that occurred in the summer of 2010.

MISSE PEACE Polymers Atomic Oxygen Erosion Results

NASA Technical Reports Server (NTRS)

deGroh, Kim, K.; Banks, Bruce A.; McCarthy, Catherine E.; Rucker, Rochelle N.; Roberts, Lily M.; Berger, Lauren A.

2006-01-01

Forty-one different polymer samples, collectively called the Polymer Erosion and Contamination Experiment (PEACE) Polymers, have been exposed to the low Earth orbit (LEO) environment on the exterior of the International Space Station (ISS) for nearly 4 years as part of Materials International Space Station Experiment 2 (MISSE 2). The objective of the PEACE Polymers experiment was to determine the atomic oxygen erosion yield of a wide variety of polymeric materials after long term exposure to the space environment. The polymers range from those commonly used for spacecraft applications, such as Teflon (DuPont) FEP, to more recently developed polymers, such as high temperature polyimide PMR (polymerization of monomer reactants). Additional polymers were included to explore erosion yield dependence upon chemical composition. The MISSE PEACE Polymers experiment was flown in MISSE Passive Experiment Carrier 2 (PEC 2), tray 1, on the exterior of the ISS Quest Airlock and was exposed to atomic oxygen along with solar and charged particle radiation. MISSE 2 was successfully retrieved during a space walk on July 30, 2005, during Discovery s STS-114 Return to Flight mission. Details on the specific polymers flown, flight sample fabrication, pre-flight and post-flight characterization techniques, and atomic oxygen fluence calculations are discussed along with a summary of the atomic oxygen erosion yield results. The MISSE 2 PEACE Polymers experiment is unique because it has the widest variety of polymers flown in LEO for a long duration and provides extremely valuable erosion yield data for spacecraft design purposes.

Space Science Payloads Optical Properties Monitor (OPM) Mission Flight Anomalies Thermal Analyses

NASA Technical Reports Server (NTRS)

Schmitz, Craig P.

2001-01-01

The OPM was the first space payload that measured in-situ the optical properties of materials and had data telemetered to ground. The OPM was EVA mounted to the Mir Docking Module for an eight-month stay where flight samples were exposed to the Mir induced and natural environments. The OPM was comprised of three optical instruments; a total hemispherical spectral reflectometer, a vacuum ultraviolet spectrometer, and a total integrated scatterometer. There were also three environmental monitors; an atomic oxygen monitor, solar and infrared radiometers, and two temperature-controlled quartz crystal microbalances (to monitor contamination). Measurements were performed weekly and data telemetered to ground through the Mir data system. This paper will describe the OPM thermal control design and how the thermal math models were used to analyze anomalies which occurred during the space flight mission.

NUTRITION w/Repository

NASA Image and Video Library

2009-06-06

ISS020-E-007566 (6 June 2009) --- European Space Agency astronaut Frank De Winne, Expedition 20 flight engineer, prepares to put samples in the Minus Eighty Laboratory Freezer for ISS (MELFI) in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

Breast Cancer Research at NASA

NASA Technical Reports Server (NTRS)

1998-01-01

Breast tissue specimens in traditional sample dishes. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunourous tissues.

Technology Development for a Hyperspectral Microwave Atmospheric Sounder (HyMAS)

NASA Technical Reports Server (NTRS)

Blackwell, W.; Galbraith, C.; Hilliard, L.; Racette, P.; Thompson, E.

2014-01-01

The Hyperspectral Microwave Atmospheric Sounder (HyMAS) is being developed at Lincoln Laboratories and accommodated by the Goddard Space Flight Center for a flight opportunity on a NASA research aircraft. The term hyperspectral microwave is used to indicate an all-weather sounding instrument that performs equivalent to hyperspectral infrared sounders in clear air with vertical resolution of approximately 1 km. Deploying the HyMAS equipped scanhead with the existing Conical Scanning Microwave Imaging Radiometer (CoSMIR) shortens the path to a flight demonstration. Hyperspectral microwave is achieved through the use of independent RF antennas that sample the volume of the Earths atmosphere through various levels of frequencies, thereby producing a set of dense, spaced vertical weighting functions.

Reduced-gravity environment hardware demonstrations of a prototype miniaturized flow cytometer and companion microfluidic mixing technology.

PubMed

Phipps, William S; Yin, Zhizhong; Bae, Candice; Sharpe, Julia Z; Bishara, Andrew M; Nelson, Emily S; Weaver, Aaron S; Brown, Daniel; McKay, Terri L; Griffin, DeVon; Chan, Eugene Y

2014-11-13

Until recently, astronaut blood samples were collected in-flight, transported to earth on the Space Shuttle, and analyzed in terrestrial laboratories. If humans are to travel beyond low Earth orbit, a transition towards space-ready, point-of-care (POC) testing is required. Such testing needs to be comprehensive, easy to perform in a reduced-gravity environment, and unaffected by the stresses of launch and spaceflight. Countless POC devices have been developed to mimic laboratory scale counterparts, but most have narrow applications and few have demonstrable use in an in-flight, reduced-gravity environment. In fact, demonstrations of biomedical diagnostics in reduced gravity are limited altogether, making component choice and certain logistical challenges difficult to approach when seeking to test new technology. To help fill the void, we are presenting a modular method for the construction and operation of a prototype blood diagnostic device and its associated parabolic flight test rig that meet the standards for flight-testing onboard a parabolic flight, reduced-gravity aircraft. The method first focuses on rig assembly for in-flight, reduced-gravity testing of a flow cytometer and a companion microfluidic mixing chip. Components are adaptable to other designs and some custom components, such as a microvolume sample loader and the micromixer may be of particular interest. The method then shifts focus to flight preparation, by offering guidelines and suggestions to prepare for a successful flight test with regard to user training, development of a standard operating procedure (SOP), and other issues. Finally, in-flight experimental procedures specific to our demonstrations are described.

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

PubMed Central

Bae, Candice; Sharpe, Julia Z.; Bishara, Andrew M.; Nelson, Emily S.; Weaver, Aaron S.; Brown, Daniel; McKay, Terri L.; Griffin, DeVon; Chan, Eugene Y.

2014-01-01

Until recently, astronaut blood samples were collected in-flight, transported to earth on the Space Shuttle, and analyzed in terrestrial laboratories. If humans are to travel beyond low Earth orbit, a transition towards space-ready, point-of-care (POC) testing is required. Such testing needs to be comprehensive, easy to perform in a reduced-gravity environment, and unaffected by the stresses of launch and spaceflight. Countless POC devices have been developed to mimic laboratory scale counterparts, but most have narrow applications and few have demonstrable use in an in-flight, reduced-gravity environment. In fact, demonstrations of biomedical diagnostics in reduced gravity are limited altogether, making component choice and certain logistical challenges difficult to approach when seeking to test new technology. To help fill the void, we are presenting a modular method for the construction and operation of a prototype blood diagnostic device and its associated parabolic flight test rig that meet the standards for flight-testing onboard a parabolic flight, reduced-gravity aircraft. The method first focuses on rig assembly for in-flight, reduced-gravity testing of a flow cytometer and a companion microfluidic mixing chip. Components are adaptable to other designs and some custom components, such as a microvolume sample loader and the micromixer may be of particular interest. The method then shifts focus to flight preparation, by offering guidelines and suggestions to prepare for a successful flight test with regard to user training, development of a standard operating procedure (SOP), and other issues. Finally, in-flight experimental procedures specific to our demonstrations are described. PMID:25490614

Breast Cancer Research at NASA

NASA Technical Reports Server (NTRS)

1998-01-01

Isolation of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Isolate of long-term growth human mammary epithelial cells (HMEC) from outgrowth of duct element; cells shown soon after isolation and early in culture in a dish. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cell (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunorous tissue. Credit: Dr. Robert Tichmond, NASA/Marshall Space Flight Center (MSFC).

Microgravity

NASA Image and Video Library

1998-10-10

Isolation of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Isolate of long-term growth human mammary epithelial cells (HMEC) from outgrowth of duct element; cells shown soon after isolation and early in culture in a dish. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cell (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunorous tissue. Credit: Dr. Robert Tichmond, NASA/Marshall Space Flight Center (MSFC).

Microgravity

NASA Image and Video Library

1998-10-10

Isolation of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Outgrowth of cells from duct element in upper right corner cultured in a standard dish; most cells spontaneously die during early cell divisions, but a few will establish long-term growth. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cell (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunorous tissue. Credit: Dr. Robert Tichmond, NASA/Marshall Space Flight Center (MSFC).

Validation of Procedures for Monitoring Crewmember Immune Function SDBI-1900, SMO-015 - Integrated Immune

NASA Technical Reports Server (NTRS)

Crucian, Brian; Stowe, Raymond; Mehta, Satish; Uchakin, Peter; Nehlsen-Cannarella, Sandra; Morukov, Boris; Pierson, Duane; Sams, Clarence

2007-01-01

There is ample evidence to suggest that space flight leads to immune system dysregulation. This may be a result of microgravity, confinement, physiological stress, radiation, environment or other mission-associated factors. The clinical risk from prolonged immune dysregulation during space flight are not yet determined, but may include increased incidence of infection, allergy, hypersensitivity, hematological malignancy or altered wound healing. Each of the clinical events resulting from immune dysfunction has the potential to impact mission critical objectives during exploration-class missions. To date, precious little in-flight immune data has been generated to assess this phenomenon. The majority of recent flight immune studies have been post-flight assessments, which may not accurately reflect the in-flight condition. There are no procedures currently in place to monitor immune function or its effect on crew health. The objective of this Supplemental Medical Objective (SMO) is to develop and validate an immune monitoring strategy consistent with operational flight requirements and constraints. This SMO will assess the clinical risks resulting from the adverse effects of space flight on the human immune system and will validate a flight-compatible immune monitoring strategy. Characterization of the clinical risk and the development of a monitoring strategy are necessary prerequisite activities prior to validating countermeasures. This study will determine, to the best level allowed by current technology, the in-flight status of crewmembers immune system. Pre-flight, in-flight and post-flight assessments of immune status, immune function, viral reactivation and physiological stress will be performed. The in-flight samples will allow a distinction between legitimate in-flight alterations and the physiological stresses of landing and readaptation which are believed to alter landing day assessments. The overall status of the immune system during flight (activation, deficiency, dysregulation) and the response of the immune system to specific latent virus reactivation (known to occur during space flight) will be thoroughly assessed. Following completion of the SMO the data will be evaluated to determine the optimal set of assays for routine monitoring of crewmember immune system function, should the clinical risk warrant such monitoring.

Experiment requirements: Vitamin D metabolites and bone demineralization, Spacelab 2, experiment no. 1

NASA Technical Reports Server (NTRS)

Schnoes, H. K.; Holton, E. M.; Thirolf, R. G.

1978-01-01

As a contribution toward an understanding of the molecular basis of bone loss, mineral imbalance, and increasing fecal calcium under conditions of prolonged space flight, the blood levels of biologically active vitamin D metabolites of flight crew members will be quantitatively measured. Prior to the mission, the refinement of existing and the development of new techniques for the assay of all vitamin D metabolites will provide an arsenal of methods suitable for a wide range of metabolite levels. In terms of practical application, the analysis of human and animal plasma samples, Spacelab crew plasma samples, and flight hardware are envisioned.

Effect of long real space flight on the whole genome mRNA expression properties in medaka Oryzias latipes

NASA Astrophysics Data System (ADS)

Kozlova, Olga; Gusev, Oleg; Levinskikh, Margarita; Sychev, Vladimir; Poddubko, Svetlana

The current study is addressed to the complex analysis of whole genome mRNA expression profile and properties of splicing variants formation in different organs of medaka fish exposed to prolonged space flight in the frame of joint Russia-Japan research program “Aquarium-AQH”. The fish were kept in the AQH joint-aquariums system in October-December 2013, followed by fixation in RNA-preserving buffers and freezing during the space flight. The samples we returned to the Earth frozen in March 2013 and mRNAs from four fish were sequenced in organ-specific manner using HiSeq Illumina sequencing platform. The ground group fish treated in the same way was used as a control. The comparison between the groups revealed space group-specific specific mRNA expression pattern. More than 50 genes (including several types of myosins) were down-regulated in the space group. Moreover, we found an evidence for formation of space group-specific splicing variants of mRNA. Taking together, the data suggest that in spite of aquatic environment, space flight-associated factors have a strong effect on the activity of fish genome. This work was supported in part by subsidy of the Russian Government to support the Program of competitive growth of Kazan Federal University among world class academic centres and universities.

Parmitano in HTV-4

NASA Image and Video Library

2013-08-09

ISS036-E-030115 (9 Aug. 2013) --- European Space Agency astronaut Luca Parmitano, Expedition 36 flight engineer, using a Russian AK-1M absorber, samples the air in the newly attached Japanese "Kounotori" H2 Transfer Vehicle-4 (HTV-4) docked to the International Space Station's Harmony node.

Urine sampling and collection system

NASA Technical Reports Server (NTRS)

Fogal, G. L.; Mangialardi, J. K.; Reinhardt, C. G.

1971-01-01

This specification defines the performance and design requirements for the urine sampling and collection system engineering model and establishes requirements for its design, development, and test. The model shall provide conceptual verification of a system applicable to manned space flight which will automatically provide for collection, volume sensing, and sampling of urine.

Hubble Space telescope thermal cycle test report for large solar array samples with BSFR cells (Sample numbers 703 and 704)

NASA Technical Reports Server (NTRS)

Alexander, D. W.

1992-01-01

The Hubble space telescope (HST) solar array was designed to meet specific output power requirements after 2 years in low-Earth orbit, and to remain operational for 5 years. The array, therefore, had to withstand 30,000 thermal cycles between approximately +100 and -100 C. The ability of the array to meet this requirement was evaluated by thermal cycle testing, in vacuum, two 128-cell solar cell modules that exactly duplicated the flight HST solar array design. Also, the ability of the flight array to survive an emergency deployment during the dark (cold) portion of an orbit was evaluated by performing a cold-roll test using one module.

Sample Analysis at Mars Instrument, Side Panels Off

NASA Image and Video Library

2012-08-27

An instrument suite that will analyze the chemical ingredients in samples of Martian atmosphere, rocks and soil during the mission of NASA Mars rover Curiosity, is shown here during assembly at NASA Goddard Space Flight Center, Greenbelt, Md., in 2010.

Comparison of chromosome aberration frequencies in pre- and post-flight astronaut lymphocytes irradiated in vitro with gamma rays

NASA Technical Reports Server (NTRS)

Wu, H.; George, K.; Willingham, V.; Cucinotta, F. A.

2001-01-01

If radiosensitivity is altered in a microgravity environment, it will affect the accuracy of assessing astronauts' risk from exposure to space radiation. To investigate the effects of space flight on radiosensitivity, we exposed a crewmember's blood to gamma rays at doses ranging from 0 to 3 Gy and analyzed chromosome aberrations in mitotic lymphocytes. The blood samples were collected 10 days prior to an 8-day Shuttle mission, the day the flight returned, and 14 days after the flight. After exposure, lymphocytes were stimulated to grow in media containing phytohaemagglutinin (PHA) and mitotic cells were harvested for chromosome analysis using a fluorescence in situ hybridization (FISH) with whole chromosome specific probes. The dose response of total exchanges showed no changes in the radiosensitivity after the mission.

Solidification of eutectic system alloys in space (M-19)

NASA Technical Reports Server (NTRS)

Ohno, Atsumi

1993-01-01

It is well known that in the liquid state eutectic alloys are theoretically homogeneous under 1 g conditions. However, the homogeneous solidified structure of this alloy is not obtained because thermal convection and non-equilibrium solidification occur. The present investigators have clarified the solidification mechanisms of the eutectic system alloys under 1 g conditions by using the in situ observation method; in particular, the primary crystals of the eutectic system alloys never nucleated in the liquid, but instead did so on the mold wall, and the crystals separated from the mold wall by fluid motion caused by thermal convection. They also found that the equiaxed eutectic grains (eutectic cells) are formed on the primary crystals. In this case, the leading phase of the eutectic must agree with the phase of the primary crystals. In space, no thermal convection occurs so that primary crystals should not move from the mold wall and should not appear inside the solidified structure. Therefore no equiaxed eutectic grains will be formed under microgravity conditions. Past space experiments concerning eutectic alloys were classified into two types of experiments: one with respect to the solidification mechanisms of the eutectic alloys and the other to the unidirectional solidification of this alloy. The former type of experiment has the problem that the solidified structures between microgravity and 1 g conditions show little difference. This is why the flight samples were prepared by the ordinary cast techniques on Earth. Therefore it is impossible to ascertain whether or not the nucleation and growth of primary crystals in the melt occur and if primary crystals influence the formation of the equiaxed eutectic grains. In this experiment, hypo- and hyper-eutectic aluminum copper alloys which are near eutectic point are used. The chemical compositions of the samples are Al-32.4mass%Cu (Hypo-eutectic) and Al-33.5mass%Cu (hyper-eutectic). Long rods for the samples are cast by the Ohno Continuous Casting Process and they show the unidirectionally solidified structure. Each flight and ground sample was made of these same rods. The dimensions of all samples are 4.5 mm in diameter and 23.5 mm in length. Each sample is put in a graphite capsule and then vacuum sealed in a double silica ampoule. Then the ampoule is put in the tantalum cartridge and sealed by electron beam welding. For onbard experiments, a Continuous Heating Furnance (CHF) will be used for melting and solidifying samples under microgravity conditions. Six flight samples will be used. Four samples are hypo-eutectic and two are hyper-eutectic alloys. The surface of the two hypo-eutectic alloy samples are covered with aluminum oxide film to prevent Marangoni convection expected under microgravity conditions. Each sample will be heated to 700 C and held at that temperature for 5 min. After that the samples will be allowed to cool to 500 C in the furnace and they will be taken out of the furnace for He gas cooling. The heating and cooling diagrams for the flight experiments are shown. After collecting the flight samples, the solidified structures of the samples will be examined and the mechanisms of eutectic solidification under microgravity conditions will be determined. It is likely that successful flight experiment results will lead to production of high quality eutectic alloys and eutectic composite materials in space.

14 CFR 1214.1705 - Selection of space flight participants.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Selection of space flight participants. 1214.1705 Section 1214.1705 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT Space Flight Participants § 1214.1705 Selection of space flight participants. (a) The agency will...

14 CFR 1214.1705 - Selection of space flight participants.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Selection of space flight participants. 1214.1705 Section 1214.1705 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT Space Flight Participants § 1214.1705 Selection of space flight participants. (a) The agency will...

Free-decay time-domain modal identification for large space structures

NASA Technical Reports Server (NTRS)

Kim, Hyoung M.; Vanhorn, David A.; Doiron, Harold H.

1992-01-01

Concept definition studies for the Modal Identification Experiment (MIE), a proposed space flight experiment for the Space Station Freedom (SSF), have demonstrated advantages and compatibility of free-decay time-domain modal identification techniques with the on-orbit operational constraints of large space structures. Since practical experience with modal identification using actual free-decay responses of large space structures is very limited, several numerical and test data reduction studies were conducted. Major issues and solutions were addressed, including closely-spaced modes, wide frequency range of interest, data acquisition errors, sampling delay, excitation limitations, nonlinearities, and unknown disturbances during free-decay data acquisition. The data processing strategies developed in these studies were applied to numerical simulations of the MIE, test data from a deployable truss, and launch vehicle flight data. Results of these studies indicate free-decay time-domain modal identification methods can provide accurate modal parameters necessary to characterize the structural dynamics of large space structures.

The Student Spaceflight Experiments Program: Access to the ISS for K-14 Students

NASA Astrophysics Data System (ADS)

Livengood, Timothy A.; Goldstein, J. J.; Hamel, S.; Manber, J.; Hulslander, M.

2013-10-01

The Student Spaceflight Experiments Program (SSEP) has flown 53 experiments to space, on behalf of students from middle school through community college, on 4 missions: each of the last 2 Space Shuttle flights, the first SpaceX demonstration flight to the International Space Station (ISS), and on SpaceX-1 to ISS. Two more missions to ISS have payloads flying in Fall 2013. SSEP plans 2 missions to the ISS per year for the foreseeable future, and is expanding the program to include 4-year undergraduate college students and home-schooled students. SSEP experiments have explored biological, chemical, and physical phenomena within self-contained enclosures developed by NanoRacks, currently in the form of MixStix Fluid Mixing Enclosures. 21,600 students participated in the initial 6 missions of SSEP, directly experiencing the entire lifecycle of space science experimentation through community-wide participation in SSEP, taking research from a nascent idea through developing competitive research proposals, down-selecting to three proposals from each participating community and further selection of a single proposal for flight, actual space flight, sample recovery, analysis, and reporting. The National Air and Space Museum has hosted 3 National Conferences for SSEP student teams to report results in keeping with the model of professional research. Student teams have unflinchingly reported on success, failure, and groundbased efforts to develop proposals for future flight opportunities. Community participation extends outside the sciences and the immediate proposal efforts to include design competitions for mission patches, which also fly to space. Student experimenters have rallied around successful proposal teams to support a successful experiment on behalf of the entire community. SSEP is a project of the National Center for Earth and Space Science Education enabled through NanoRacks LLC, working in partnership with NASA under a Space Act Agreement as part of the utilization of the International Space Station as a National Laboratory.

Flexible imaging payload for real-time fluorescent biological imaging in parabolic, suborbital and space analog environments

NASA Astrophysics Data System (ADS)

Bamsey, Matthew T.; Paul, Anna-Lisa; Graham, Thomas; Ferl, Robert J.

2014-10-01

Fluorescent imaging offers the ability to monitor biological functions, in this case biological responses to space-related environments. For plants, fluorescent imaging can include general health indicators such as chlorophyll fluorescence as well as specific metabolic indicators such as engineered fluorescent reporters. This paper describes the Flex Imager a fluorescent imaging payload designed for Middeck Locker deployment and now tested on multiple flight and flight-related platforms. The Flex Imager and associated payload elements have been developed with a focus on 'flexibility' allowing for multiple imaging modalities and change-out of individual imaging or control components in the field. The imaging platform is contained within the standard Middeck Locker spaceflight form factor, with components affixed to a baseplate that permits easy rearrangement and fine adjustment of components. The Flex Imager utilizes standard software packages to simplify operation, operator training, and evaluation by flight provider flight test engineers, or by researchers processing the raw data. Images are obtained using a commercial cooled CCD image sensor, with light-emitting diodes for excitation and a suite of filters that allow biological samples to be imaged over wavelength bands of interest. Although baselined for the monitoring of green fluorescent protein and chlorophyll fluorescence from Arabidopsis samples, the Flex Imager payload permits imaging of any biological sample contained within a standard 10 cm by 10 cm square Petri plate. A sample holder was developed to secure sample plates under different flight profiles while permitting sample change-out should crewed operations be possible. In addition to crew-directed imaging, autonomous or telemetric operation of the payload is also a viable operational mode. An infrared camera has also been integrated into the Flex Imager payload to allow concurrent fluorescent and thermal imaging of samples. The Flex Imager has been utilized to assess, in real-time, the response of plants to novel environments including various spaceflight analogs, including several parabolic flight environments as well as hypobaric plant growth chambers. Basic performance results obtained under these operational environments, as well as laboratory-based tests are described. The Flex Imager has also been designed to be compatible with emerging suborbital platforms.

Thirsk in JPM

NASA Image and Video Library

2009-06-06

ISS020-E-007147 (6 June 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

Thirsk in JPM

NASA Image and Video Library

2009-06-06

ISS020-E-007140 (6 June 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

MELFI ops

NASA Image and Video Library

2013-06-06

Astronaut Karen Nyberg,Expedition 36 flight engineer,works with samples in Minus Eighty-Degree Laboratory Freezer for ISS (MELFI-3) in the Destiny laboratory of the Earth-orbiting International Space Station.

EXPOSE-R2: The Astrobiological ESA Mission on Board of the International Space Station.

PubMed

Rabbow, Elke; Rettberg, Petra; Parpart, Andre; Panitz, Corinna; Schulte, Wolfgang; Molter, Ferdinand; Jaramillo, Esther; Demets, René; Weiß, Peter; Willnecker, Rainer

2017-01-01

On July 23, 2014, the Progress cargo spacecraft 56P was launched from Baikonur to the International Space Station (ISS), carrying EXPOSE-R2, the third ESA (European Space Agency) EXPOSE facility, the second EXPOSE on the outside platform of the Russian Zvezda module, with four international astrobiological experiments into space. More than 600 biological samples of archaea, bacteria (as biofilms and in planktonic form), lichens, fungi, plant seeds, triops eggs, mosses and 150 samples of organic compounds were exposed to the harsh space environment and to parameters similar to those on the Mars surface. Radiation dosimeters distributed over the whole facility complemented the scientific payload. Three extravehicular activities later the chemical samples were returned to Earth on March 2, 2016, with Soyuz 44S, having spent 588 days in space. The biological samples arrived back later, on June 18, 2016, with 45S, after a total duration in space of 531 days. The exposure of the samples to Low Earth Orbit vacuum lasted for 531 days and was divided in two parts: protected against solar irradiation during the first 62 days, followed by exposure to solar radiation during the subsequent 469 days. In parallel to the space mission, a Mission Ground Reference (MGR) experiment with a flight identical Hardware and a complete flight identical set of samples was performed at the premises of DLR (German Aerospace Center) in Cologne by MUSC (Microgravity User Support Center), according to the mission data either downloaded from the ISS (temperature data, facility status, inner pressure status) or provided by RedShift Design and Engineering BVBA, Belgium (calculated ultra violet radiation fluence data). In this paper, the EXPOSE-R2 facility, the experimental samples, mission parameters, environmental parameters, and the overall mission and MGR sequences are described, building the background for the research papers of the individual experiments, their analysis and results.

EXPOSE-R2: The Astrobiological ESA Mission on Board of the International Space Station

PubMed Central

Rabbow, Elke; Rettberg, Petra; Parpart, Andre; Panitz, Corinna; Schulte, Wolfgang; Molter, Ferdinand; Jaramillo, Esther; Demets, René; Weiß, Peter; Willnecker, Rainer

2017-01-01

On July 23, 2014, the Progress cargo spacecraft 56P was launched from Baikonur to the International Space Station (ISS), carrying EXPOSE-R2, the third ESA (European Space Agency) EXPOSE facility, the second EXPOSE on the outside platform of the Russian Zvezda module, with four international astrobiological experiments into space. More than 600 biological samples of archaea, bacteria (as biofilms and in planktonic form), lichens, fungi, plant seeds, triops eggs, mosses and 150 samples of organic compounds were exposed to the harsh space environment and to parameters similar to those on the Mars surface. Radiation dosimeters distributed over the whole facility complemented the scientific payload. Three extravehicular activities later the chemical samples were returned to Earth on March 2, 2016, with Soyuz 44S, having spent 588 days in space. The biological samples arrived back later, on June 18, 2016, with 45S, after a total duration in space of 531 days. The exposure of the samples to Low Earth Orbit vacuum lasted for 531 days and was divided in two parts: protected against solar irradiation during the first 62 days, followed by exposure to solar radiation during the subsequent 469 days. In parallel to the space mission, a Mission Ground Reference (MGR) experiment with a flight identical Hardware and a complete flight identical set of samples was performed at the premises of DLR (German Aerospace Center) in Cologne by MUSC (Microgravity User Support Center), according to the mission data either downloaded from the ISS (temperature data, facility status, inner pressure status) or provided by RedShift Design and Engineering BVBA, Belgium (calculated ultra violet radiation fluence data). In this paper, the EXPOSE-R2 facility, the experimental samples, mission parameters, environmental parameters, and the overall mission and MGR sequences are described, building the background for the research papers of the individual experiments, their analysis and results. PMID:28861052

NUTRITION w/Repository

NASA Image and Video Library

2009-06-06

ISS020-E-007577 (6 June 2009) --- European Space Agency astronaut Frank De Winne, Expedition 20 flight engineer, returns a dewar tray to the Minus Eighty Laboratory Freezer for ISS (MELFI) after inserting biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

14 CFR § 1214.1705 - Selection of space flight participants.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Selection of space flight participants. § 1214.1705 Section § 1214.1705 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT Space Flight Participants § 1214.1705 Selection of space flight participants. (a) The...

14 CFR 435.8 - Human space flight.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Human space flight. 435.8 Section 435.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Human space flight. An applicant for a license to conduct a reentry with flight crew or a space flight...

14 CFR 435.8 - Human space flight.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Human space flight. 435.8 Section 435.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Human space flight. An applicant for a license to conduct a reentry with flight crew or a space flight...

14 CFR 435.8 - Human space flight.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Human space flight. 435.8 Section 435.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Human space flight. An applicant for a license to conduct a reentry with flight crew or a space flight...

14 CFR 435.8 - Human space flight.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Human space flight. 435.8 Section 435.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Human space flight. An applicant for a license to conduct a reentry with flight crew or a space flight...

14 CFR 435.8 - Human space flight.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Human space flight. 435.8 Section 435.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Human space flight. An applicant for a license to conduct a reentry with flight crew or a space flight...

Vascular Blood Collection protocol samples into MELFI

NASA Image and Video Library

2011-10-18

iss029e028495 (10/18/2011) --- Japan Aerospace Exploration Agency astronaut Satoshi Furukawa,Expedition 29 flight engineer,prepares to put samples from the CSA (Canadian Space Agency) Vascular Blood Collection protocol into the MELFI-1 (Minus Eighty Laboratory Freezer for ISS 1) unit.

Characterization of neurospora circadian rhythms in space

NASA Technical Reports Server (NTRS)

Ferraro, James S.

1987-01-01

To determine whether the circadian rhythm of conidiation in neurospora crassa is endogenously derived or is driven by some geophysical time cue, an experiment was conducted on space shuttle flight STS-9, where inoculated race tubes were exposed to the microgravity environment of space. The results demonstated that the rhythm can persist in space. However, there were several minor alterations noted; an increase in the period of the oscillation and the variability of the growth rate and a diminished rhythm amplitude, which eventually damped out in 25% of the flight tubes. On day seven of the flight, the tubes were exposed to light while their growth fronts were marked. It appears that some aspects of this marking process reinstated a robust rhythm in all the tubes which continued throughout the remainder of the flight. It was hypothesized that the damping found prior to the marking procedure on STS-9 may have been a result of the hypergravity pulse of launch and not due to the microgravity of the orbital lab; furthermore, that the marking procedure, by exposing the samples to light, had reinstated rhythmicity. To test this, an investigation was conducted into the effects of acute and chronic exposure to hypergravity.

Are Samples Obtained after Return to Earth Reflective of Spaceflight or Increased Gravity?

NASA Technical Reports Server (NTRS)

Wade, C. R.; Holton, E.; Baer, L.; Moran, M.

2001-01-01

Upon return to Earth, following space flight, living systems are immediately exposed to an increase in gravity of 1G. It has been difficult to differentiate between changes that are residuals of the acclimation to space flight from those resulting from acute exposure to an increase in =gravity upon re-entry. We compared previously reported changes observed in male Sprague-Dawley rats upon return to Earth to those induced by centrifugation, because both paradigms result in an increase of 1G. With both treatments there was a reduction in body mass, due to reduced food intake and increased urine output. The decrease in food intake was initially greater with centrifugation. The magnitudes of the changes in food intake and urine output were similar in both treatments. However, the slightly greater initial loss in body mass with centrifugation was due to a decrease in water intake not seen after space flight. The absence of pronounced differences between these treatments suggest the responses observed after landing are not residuals of adaptation to the space flight environment, but the result of adaptation to an increase in the level of gravity.

Biomedical Analyses of Mice Body Hair Exposed to Long-term Space Flight as a Compliment of Human Research

NASA Astrophysics Data System (ADS)

Mukai, Chiaki

Introduction: To understand the effect of space environment characterized by microgravity and radiation on protein and mineral metabolisms is important for developing the countermeasures to the adverse effects happening on the astronauts who stay long-term in space. Thus JAXA has started a human research to study the effects of long-term exposure in space flight on gene expression and mineral metabolism by analyzing astronaut's hair grown in space since December 2009 (Experiment nicknamed "HAIR"). Ten human subjects who are the crew of the International Space Station (ISS) will be expected to complete this experiment. Thanks to the tissue sharing program of space-flown mice which is presented and organized by AGI(Italian Space Agency), we can also have an opportunity to analyze rodents samples which will greatly compliment human hair experiment by enable us to conduct more detailed analysis with the expansion of skin analysis which is not include in human experiment. The purpose of this flown-mice experiment is to study the effects of long-term exposure to space environment such as microgravity and space radiation on mineral and protein metabolism, the biological responses to the stress levels, and the initial process of skin carcinogenesis by analyzing hair shaft, its root cells, and skin. Approach and Method In this experiment, we analyzed hair shaft, hair root and skin. Hair samples with skin were taken from 3-month space-flown mice and ground-control mice in the AGI's tissue sharing program in 2009. The sample numbers of space-flown mice and control-mice were three and six, respectively. And they were at the Mice Drawer System (MDS) in ISS and in the laboratory of Geneva University. For the hair shaft, the mineral balance is investi-gated by energy dispersive X-ray spectroscopy (SEM-EDX). For hair root, the extracted RNA undergoes DNA microarray analysis, and will be further examined particular interests of gene-expression by real time Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. DNA is also extracted from the same samples and mitochondrial DNA copy numbers are ex-amined. For mice skin, the examination of the mutation was performed to investigate whether the space flight will cause the visible mutation which may reflect an evidence of promotion step of carcinogenesis by space radiation. Result: Analysis on the specimen is in progress. A brief results will be introduced during the COSPAR meeting. The mice body hair analysis will give us basic information to understand how space environment such as microgravity and radiation affect on the living organisms.

Low Gravity Freefall Facilities

NASA Technical Reports Server (NTRS)

1981-01-01

Composite of Marshall Space Flight Center's Low-Gravity Free Fall Facilities.These facilities include a 100-meter drop tower and a 100-meter drop tube. The drop tower simulates in-flight microgravity conditions for up to 4.2 seconds for containerless processing experiments, immiscible fluids and materials research, pre-flight hardware design test and flight experiment simulation. The drop tube simulates in-flight microgravity conditions for up to 4.6 seconds and is used extensively for ground-based microgravity convection research in which extremely small samples are studied. The facility can provide deep undercooling for containerless processing experiments that require materials to remain in a liquid phase when cooled below the normal solidification temperature.

Microgravity

NASA Image and Video Library

1981-03-30

Composite of Marshall Space Flight Center's Low-Gravity Free Fall Facilities.These facilities include a 100-meter drop tower and a 100-meter drop tube. The drop tower simulates in-flight microgravity conditions for up to 4.2 seconds for containerless processing experiments, immiscible fluids and materials research, pre-flight hardware design test and flight experiment simulation. The drop tube simulates in-flight microgravity conditions for up to 4.6 seconds and is used extensively for ground-based microgravity convection research in which extremely small samples are studied. The facility can provide deep undercooling for containerless processing experiments that require materials to remain in a liquid phase when cooled below the normal solidification temperature.

Pristine and Surface-Modified Polymers in LEO: MISSE Results versus Predictive Models and Ground-Based Testing

NASA Astrophysics Data System (ADS)

Iskanderova, Zelina; Kleiman, Jacob I.; Tennyson, Rod C.

2009-01-01

Space flight data, collected and published by NASA Glenn Research Center (GRC) team for a set of pristine polymeric materials selected, compiled, and tested in two LEO flight experiments at the International Space Station, as part of the "Materials International Space Station Experiment" (MISSE), has been used for comparison with previously developed atomic oxygen erosion predictive models. The same set of materials was used for a ground-based fast atomic beam (FAO) experimental erosion study at ITL/UTIAS, where the FAO exposure was performed mostly at a standard fluence of 2×1020 cm-2, with the results collected in a database for the development of a prototype of predictive software. A comparison of MISSE-1 flight data with two predictive correlations has shown good agreement, confirming the developed approach to polymers erosion resistance forecast that might be used also for newly developed or untested in space polymeric materials. A number of surface-modified thin film space polymers, treated by two ITL-developed and patented surface modification technologies, Implantox™ [5] and Photosil™ [6], have been also included in MISSE flight experiment. The results from those MISSE samples have shown full protection of AO-sensitive main space-related hydrocarbon polymers, such as Kapton HN, back-metalized Kapton H and Kapton E, and Mylar, when treated by Implantox™ surface modification technology and significant erosion resistance enhancement up to full protection by Photosil™ treatment.

Reiter works with SWAB ASD Filter Kit in the U.S. Laboratory during Expedition 13

NASA Image and Video Library

2006-09-10

ISS013-E-80066 (10 Sept. 2006) --- European Space Agency (ESA) astronaut Thomas Reiter, Expedition 13 flight engineer, works with the surface, water and air biocharacterization (SWAB) air sampling device (ASD) filter kit in the Destiny laboratory of the International Space Station.

Gerst with MSG during BASS session

NASA Image and Video Library

2014-06-13

ISS040-E-011004 (13 June 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, works with samples and hardware for a combustion experiment known as the Burning and Suppression of Solids (BASS) in the Microgravity Science Glovebox (MSG) in the Destiny laboratory of the International Space Station.

Gerst with MSG during BASS session

NASA Image and Video Library

2014-06-13

ISS040-E-011006 (13 June 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, works with samples and hardware for a combustion experiment known as the Burning and Suppression of Solids (BASS) in the Microgravity Science Glovebox (MSG) in the Destiny laboratory of the International Space Station.

Latent virus reactivation in astronauts on the international space station.

PubMed

Mehta, Satish K; Laudenslager, Mark L; Stowe, Raymond P; Crucian, Brian E; Feiveson, Alan H; Sams, Clarence F; Pierson, Duane L

2017-01-01

Reactivation of latent herpes viruses was measured in 23 astronauts (18 male and 5 female) before, during, and after long-duration (up to 180 days) spaceflight onboard the international space station . Twenty age-matched and sex-matched healthy ground-based subjects were included as a control group. Blood, urine, and saliva samples were collected before, during, and after spaceflight. Saliva was analyzed for Epstein-Barr virus, varicella-zoster virus, and herpes simplex virus type 1. Urine was analyzed for cytomegalovirus. One astronaut did not shed any targeted virus in samples collected during the three mission phases. Shedding of Epstein-Barr virus, varicella-zoster virus, and cytomegalovirus was detected in 8 of the 23 astronauts. These viruses reactivated independently of each other. Reactivation of Epstein-Barr virus, varicella-zoster virus, and cytomegalovirus increased in frequency, duration, and amplitude (viral copy numbers) when compared to short duration (10 to 16 days) space shuttle missions. No evidence of reactivation of herpes simplex virus type 1, herpes simplex virus type 2, or human herpes virus 6 was found. The mean diurnal trajectory of salivary cortisol changed significantly during flight as compared to before flight ( P  = 0.010). There was no statistically significant difference in levels of plasma cortisol or dehydoepiandosterone concentrations among time points before, during, and after flight for these international space station crew members, although observed cortisol levels were lower at the mid and late-flight time points. The data confirm that astronauts undertaking long-duration spaceflight experience both increased latent viral reactivation and changes in diurnal trajectory of salivary cortisol concentrations.

Breast Cancer Research at NASA

NASA Technical Reports Server (NTRS)

1998-01-01

Isolation of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Outgrowth of cells from duct element in upper right corner cultured in a standard dish; most cells spontaneously die during early cell divisions, but a few will establish long-term growth. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cell (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunorous tissue. Credit: Dr. Robert Tichmond, NASA/Marshall Space Flight Center (MSFC).

Breast Cancer Research at NASA

NASA Technical Reports Server (NTRS)

1998-01-01

Isolation of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Same long-term growth human mammary epithelial cells (HMEC), but after 3 weeks in concinuous culture. Note attempts to reform duct elements, but this time in two dimensions in a dish rather that in three demensions in tissue. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cell (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunorous tissue. Credit: Dr. Robert Tichmond, NASA/Marshall Space Flight Center (MSFC).

Microgravity

NASA Image and Video Library

1998-10-10

Isolation of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Same long-term growth human mammary epithelial cells (HMEC), but after 3 weeks in concinuous culture. Note attempts to reform duct elements, but this time in two dimensions in a dish rather that in three demensions in tissue. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cell (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunorous tissue. Credit: Dr. Robert Tichmond, NASA/Marshall Space Flight Center (MSFC).

Directional Solidification of Bi-Sn on USMP-4

NASA Technical Reports Server (NTRS)

Abbaschian, Reza; deGroh, H., III; Leonardi, E.; Timchenko, V.; deVahlDavis, G.

1999-01-01

The experiments used MEPHISTO hardware to study the solidification and melting behavior of bismuth alloyed with 1 at% tin. Three samples, each approximately 900 mm long and 6mm in diameter, were used. A portion of each sample also included a 2 mm diameter growth capillary, to assist in the formation of a single grain. One sample provided the Seebeck voltage generated during melting and freezing processes. Another provided temperature data and Peltier pulsed demarcation of the interface shape for post flight analysis. The third sample provided resistance and growth velocity measurements, as well as additional thermal data. The third sample was also quenched at the end of the mission to preserve the composition of the liquid near the interface for post flight determination. A total of 450mm of directionally solidified samples were preserved for post mission structural and compositional characterization. Substantial differences were observed in the Seebeck signal between the ground-based experiments and the space-based experiments. The temperature gradient in the liquid for the ground-based experiments was significantly lower than the temperature gradient in the liquid for the space-based experiments.

Preliminary Results from the Flight of the Solar Array Module Plasma Interactions Experiment (SAMPIE)

NASA Technical Reports Server (NTRS)

Ferguson, Dale C.; Hillard, G. Barry

1994-01-01

SAMPIE, the Solar Array Module Plasma Interactions Experiment, flew in the Space Shuttle Columbia payload bay as part of the OAST-2 mission on STS-62, March, 1994. SAMPIE biased samples of solar arrays and space power materials to varying potentials with respect to the surrounding space plasma, and recorded the plasma currents collected and the arcs which occurred, along with a set of plasma diagnostics data. A large set of high quality data was obtained on the behavior of solar arrays and space power materials in the space environment. This paper is the first report on the data SAMPIE telemetered to the ground during the mission. It will be seen that the flight data promise to help determine arcing thresholds, snapover potentials and floating potentials for arrays and spacecraft in LEO.

Antibodies to myofibril antigens in cosmonauts after spaceflights

NASA Technical Reports Server (NTRS)

Tashpulatov, R. Y.; Danilova, T. A.; Lesnyak, A. T.; Legenkov, V. I.; Znamenskiy, V. S.; Dedyuyeva, Y. Y.

1980-01-01

Serum samples obtained from 15 astronauts before and after spaceflights were studied with the use of the indirect immunofluorescent method. In seven astronauts antibodies to different elements of the human heart muscle appeared after flights. Strong and very strong luminescence of the elements of heart muscle tissue was detected in the astronauts after the third space flight. In a study of the sera on sections of bovine heart muscle tissue the reactions of the sera taken before and after flight were found to show no essential differences.

Preliminary results from the flight of the Solar Array Module Plasma Interactions Experiment (SAMPIE)

NASA Technical Reports Server (NTRS)

Ferguson, Dale C.; Hillard, G. Barry

1994-01-01

SAMPIE, the Solar Array Module Plasma Interactions Experiment, flew in the Space Shuttle Columbia payload bay as part of the Office of Aeronautics and Space Technology-2 (OAST-2) mission on STS-62, March, 1994. SAMPIE biased samples of solar arrays and space power materials to varying potentials with respect to the surrounding space plasma, and recorded the plasma currents collected and the arcs which occurred, along with a set of plasma diagnostics data. A large set of high quality data was obtained on the behavior of solar arrays and space power materials in the space environment. This paper is the first report on the data SAMPIE telemetered to the ground during the mission. It will be seen that the flight data promise to help determine arcing thresholds, snapover potentials, and floating potentials for arrays and spacecraft in LEO.

In Situ Monitoring of Crystal Growth Using MEPHISTO, Mission STS 87-Program USMP-4: Experimental Analysis

NASA Technical Reports Server (NTRS)

Abbaschian, Reza; Chen, F.; Mileham, J. R.; deGroh, H., III; Timchenko, V.; Leonardi, E.; deVahlDavis, G.; Coriell, S.; Cambon, G.

1999-01-01

This report summarizes the results of the In situ Monitoring of Crystal Growth Using MEPHISTO (Material por l'Etude des Phenomenes Interessant de la Solidification sur Terre et en Orbite) experiment on USMP-4. The report includes microstructural and compositional data obtained during the first year of the post flight analysis, as well as numerical simulation of the flight experiment. Additional analyses are being continued and will be reported in the near future. The experiments utilized MEPHISTO hardware to study the solidification and melting behavior of bismuth alloyed with 1 at% tin. The experiments involved repeated melting and solidification of three samples, each approximately 90 cm long and 6mm in diameter. Half of each sample also included a 2 mm. diameter growth capillary, to assist in the formation of single grain inside. One sample provided the Seebeck voltage generated during melting and freezing processes. Another one provided temperature data and Peltier pulsed demarcation of the interface shape for post flight analysis. The third sample provided resistance and velocity measurements, as well as additional thermal data. The third sample was also quenched at the end of the mission to preserve the interface composition for post flight determination. A total of more than 45cm of directionally solidified alloy were directionally solidified at the end of the flight for post mission structural and compositional characterization. Metallurgical analysis of the samples has shown that the interfacial kinetics play a key role in controlling the morphological stability of faceted alloys. Substantial differences were observed in the Seebeck signal between the ground-based experiments and the space-based experiments. The temperature gradient in the liquid for the ground-based experiments was also significantly lower than the temperature gradient in the liquid for the space-based experiments. Both of these observations indicate significant influence of liquid convection for the ground-based experiments.

iss038e053780

NASA Image and Video Library

2014-02-18

ISS038-E-053780 (18 Feb. 2014) --- NASA astronaut Rick Mastracchio, Expedition 38 flight engineer, uses a Microbial Air Sampler to collect air samples in the Unity node of the International Space Station. These air samples will be incubated for five days and tested for signs of microbial contamination.

Thermal expansion behavior of LDEF metal matrix composites

NASA Technical Reports Server (NTRS)

Le, Tuyen D.; Steckel, Gary L.

1993-01-01

The thermal expansion behavior of Long Duration Exposure Facility (LDEF) metal matrix composite materials was studied by (1) analyzing the flight data that was recorded on orbit to determine the effects of orbital time and heating/cooling rates on the performance of the composite materials, and (2) characterizing and comparing the thermal expansion behavior of post-flight LDEF and lab-control samples. The flight data revealed that structures in space are subjected to nonuniform temperature distributions, and thermal conductivity of a material is an important factor in establishing a uniform temperature distribution and avoiding thermal distortion. The flight and laboratory data showed that both Gr/Al and Gr/Mg composites were stabilized after prolonged thermal cycling on orbit. However, Gr/Al composites showed more stable thermal expansion behavior than Gr/Mg composites and offer advantages for space structures particularly where very tight thermal stability requirements in addition to high material performance must be met.

NUTRITION w/Repository

NASA Image and Video Library

2009-06-06

ISS020-E-007603 (7 June 2009) --- European Space Agency astronaut Frank De Winne, Expedition 20 flight engineer, removes a dewar tray from the Minus Eighty Laboratory Freezer for ISS (MELFI) in order to insert biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

DSO 484C, Robinson spits into a sample container

NASA Image and Video Library

1997-08-29

STS085-338-016 (7 - 19 August 1997) --- On the Space Shuttle Discovery's flight deck, astronaut Stephen K. Robinson conducts one phase of the mission's Detailed Supplementary Objectives (DSO). He uses a cotton swab to collect a saliva sample. The wrist band on his left arm is associated with the same DSO. The ongoing test, dealing with circadian rhythm and other biological systems, is in preparation for the International Space Station (ISS).

Flight Test Evaluation of the Airborne Information for Lateral Spacing (AILS) Concept

NASA Technical Reports Server (NTRS)

Abbott, Terence S.

2002-01-01

The Airborne Information for Lateral Spacing (AILS) concept is designed to support independent parallel approach operations to runways spaced as close as 2,500 feet. This report briefly describes the AILS operational concept and the results of a flight test of one implementation of this concept. The focus of this flight test experiment was to validate a prior simulator study, evaluating pilot performance, pilot acceptability, and minimum miss-distances for the rare situation in which an aircraft on one approach intrudes into the path of an aircraft on the other approach. Although the flight data set was not meant to be a statistically valid sample, the trends acquired in flight followed those of the simulator and therefore met the intent of validating the findings from the simulator. Results from this study showed that the design-goal mean miss-distance of 1,200 feet to potential collision situations was surpassed with an actual mean miss-distance of 1,859 feet. Pilot reaction times to the alerting system, which was an operational concern, averaged 0.65 seconds, were well below the design goal reaction time of 2.0 seconds. From the results of both of these tests, it can be concluded that this operational concept, with supporting technology and procedures, may provide an operationally viable means for conducting simultaneous, independent instrument approaches to runways spaced as close as 2500 ft.

Flight projects overview

NASA Technical Reports Server (NTRS)

Levine, Jack

1988-01-01

Information is given in viewgraph form on the activities of the Flight Projects Division of NASA's Office of Aeronautics and Space Technology. Information is given on space research and technology strategy, current space flight experiments, the Long Duration Exposure Facility, the Orbiter Experiment Program, the Lidar In-Space Technology Experiment, the Ion Auxiliary Propulsion System, the Arcjet Flight Experiment, the Telerobotic Intelligent Interface Flight Experiment, the Cryogenic Fluid Management Flight Experiment, the Industry/University In-Space Flight Experiments, and the Aeroassist Flight Experiment.

Spacelab

NASA Image and Video Library

1992-01-01

The IML-1 mission was the first in a series of Shuttle flights dedicated to fundamental materials and life sciences research with the international partners. The participating space agencies included: NASA, the 14-nation European Space Agency (ESA), the Canadian Space Agency (CSA), The French National Center of Space Studies (CNES), the German Space Agency and the German Aerospace Research Establishment (DAR/DLR), and the National Space Development Agency of Japan (NASDA). Dedicated to the study of life and materials sciences in microgravity, the IML missions explored how life forms adapt to weightlessness and investigated how materials behave when processed in space. Both life and materials sciences benefited from the extended periods of microgravity available inside the Spacelab science module in the cargo bay of the Space Shuttle Orbiter. This photograph shows Astronaut Norman Thagard performing the fluid experiment at the Fluid Experiment System (FES) facility inside the laboratory module. The FES facility had sophisticated optical systems for imaging fluid flows during materials processing, such as experiments to grow crystals from solution and solidify metal-modeling salts. A special laser diagnostic technique recorded the experiments, holograms were made for post-flight analysis, and video was used to view the samples in space and on the ground. Managed by the Marshall Space Flight Center (MSFC), the IML-1 mission was launched on January 22, 1992 aboard the Shuttle Orbiter Discovery (STS-42).

An instrument for spatial conductivity measurements of high Tc superconducting (HTSC) materials

NASA Technical Reports Server (NTRS)

Vansant, T.

1991-01-01

High T(sub c) Superconducting (HTSC) thin films are suggested for use in a number of aerospace applications such as an IR bolometer and as electromagnetic shielding. As part of its flight assurance role, the Materials Branch of the Goddard Space Flight Center has initiated development of an instrument capable of measuring variations in conductivity for flat samples using an eddy current testing device and an X-Y positioning table. This instrument was used to examine bulk HTSC samples. System changes that would enable characterization of thin film materials are discussed.

Human cellular immune responsiveness following space flight

NASA Technical Reports Server (NTRS)

Taylor, G. R.; Dardano, J. R.

1983-01-01

Peripheral circulating lymphocytes were separated from astronaut blood samples three times before and two times after the first four US Space Shuttle flights. The ability of the in vitro T lymphocytes to respond to Phytohemagglutinin by blastogenesis was found to be reduced for each crewmember following spaceflight. In addition, the astronauts experienced a postflight increase in neutrophils and a decrease in eosinophils. These postflight changes in leukocytes are shown to increase with subjectively-evaluated increases in the incidence of inflight stress, indicating that stress, and not hypogravity, is likely to be the major effector of these changes.

Williams loads the MELFI for the Nutrition Experiment during Expedition 15

NASA Image and Video Library

2007-06-01

ISS015-E-10572 (1 June 2007) --- Astronaut Sunita L. Williams, Expedition 15 flight engineer, inserts test samples in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as a part of the Nutritional Status Assessment (Nutrition) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

NASA Global Atmospheric Sampling Program (GASP) data report for tape VL0015, VL0016, VL0017, VL0018, VL0019, and VL0020

NASA Technical Reports Server (NTRS)

Papthakos, L. C.; Briehl, D.

1981-01-01

This is the twelfth of a series of reports which describes the data currently available from GASP, including flight routes and dates, instrumentation, data processing procedures, and data tape specifications. In-situ measurements of atmospheric ozone, cabin ozone, carbon monoxide, water vapor, particles, clouds, condensation nuclei, filter samples and related meteorological and flight information obtained during 1732 flights of aircraft N533PA, N4711U, N655PA, and VH-EBE from January 5, 1978 through October 9, 1978 are reported. These data are now available from the National Climatic Center, Asheville, NC, 22801. In addition to the GASP data, tropopause pressures obtained from time ans space interpolation of National Meteorological Center archived data for the dates of the flights are included.

MELFI Urine Sample First Insertion

NASA Image and Video Library

2009-04-11

ISS019-E-005715 (11 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

14 CFR 460.51 - Space flight participant training.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Space flight participant training. 460.51 Section 460.51 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with a Space Flight...

14 CFR 460.51 - Space flight participant training.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Space flight participant training. 460.51 Section 460.51 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with a Space Flight...

14 CFR 460.51 - Space flight participant training.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Space flight participant training. 460.51 Section 460.51 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with a Space Flight...

14 CFR 460.51 - Space flight participant training.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Space flight participant training. 460.51 Section 460.51 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with a Space Flight...

14 CFR 460.51 - Space flight participant training.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Space flight participant training. 460.51 Section 460.51 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with a Space Flight...

Methodology for Flight Relevant Arc-Jet Testing of Flexible Thermal Protection Systems

NASA Technical Reports Server (NTRS)

Mazaheri, Alireza; Bruce, Walter E., III; Mesick, Nathaniel J.; Sutton, Kenneth

2013-01-01

A methodology to correlate flight aeroheating environments to the arc-jet environment is presented. For a desired hot-wall flight heating rate, the methodology provides the arcjet bulk enthalpy for the corresponding cold-wall heating rate. A series of analyses were conducted to examine the effects of the test sample model holder geometry to the overall performance of the test sample. The analyses were compared with arc-jet test samples and challenges and issues are presented. The transient flight environment was calculated for the Hypersonic Inflatable Aerodynamic Decelerator (HIAD) Earth Atmospheric Reentry Test (HEART) vehicle, which is a planned demonstration vehicle using a large inflatable, flexible thermal protection system to reenter the Earth's atmosphere from the International Space Station. A series of correlations were developed to define the relevant arc-jet test environment to properly approximate the HEART flight environment. The computed arcjet environments were compared with the measured arc-jet values to define the uncertainty of the correlated environment. The results show that for a given flight surface heat flux and a fully-catalytic TPS, the flight relevant arc-jet heat flux increases with the arc-jet bulk enthalpy while for a non-catalytic TPS the arc-jet heat flux decreases with the bulk enthalpy.

Brassica rapa plants adapted to microgravity with reduced photosystem I and its photochemical activity

NASA Technical Reports Server (NTRS)

Jiao, Shunxing; Hilaire, Emmanuel; Paulsen, Avelina Q.; Guikema, James A.

2004-01-01

The photosynthetic apparatus contains several protein complexes, many of which are regulated by environmental conditions. In this study, the influences of microgravity on PSI and PSII in Brassica rapa plants grown aboard the space shuttle were examined. We found that Brassica plants grown in space had a normal level of growth relative to controls under similar conditions on Earth. Upon return to Earth, cotyledons were harvested and thylakoid membranes were isolated. Analysis of chlorophyll contents showed that the Chl a/b ratio (3.5) in flight cotyledons was much higher than a ratio of 2.42 in the ground controls. The flight samples also had a reduction of PSI complexes and a corresponding 30% decrease of PSI photochemical activity. Immunoblotting showed that the reaction centre polypeptides of PSI were more apparently decreased (e.g. by 24-33% for PsaA and PsaB, and 57% for PsaC) than the light-harvesting complexes. In comparison, the accumulation of PSII complex was less affected in microgravity, thus only a slight reduction in D1, D2 and LHCII was observed in protein blots. However, there was a 32% decrease of OEC1 in the flight samples, indicating a defective OEC subcomplex. In addition, an average 54% increase of the 54 kDa CF1-beta isoform was found in the flight samples, suggesting that space-grown plants suffered from certain stresses, consistent with implications of the increased Chl a/b ratio. Taken together, the results demonstrated that Brassica plants can adapt to spaceflight microgravity, but with significant alterations in chloroplast structures and photosynthetic complexes, and especially reduction of PSI and its activity.

OSIRIS-REx Flight Dynamics and Navigation Design

NASA Astrophysics Data System (ADS)

Williams, B.; Antreasian, P.; Carranza, E.; Jackman, C.; Leonard, J.; Nelson, D.; Page, B.; Stanbridge, D.; Wibben, D.; Williams, K.; Moreau, M.; Berry, K.; Getzandanner, K.; Liounis, A.; Mashiku, A.; Highsmith, D.; Sutter, B.; Lauretta, D. S.

2018-06-01

OSIRIS-REx is the first NASA mission to return a sample of an asteroid to Earth. Navigation and flight dynamics for the mission to acquire and return a sample of asteroid 101955 Bennu establish many firsts for space exploration. These include relatively small orbital maneuvers that are precise to ˜1 mm/s, close-up operations in a captured orbit about an asteroid that is small in size and mass, and planning and orbit phasing to revisit the same spot on Bennu in similar lighting conditions. After preliminary surveys and close approach flyovers of Bennu, the sample site will be scientifically characterized and selected. A robotic shock-absorbing arm with an attached sample collection head mounted on the main spacecraft bus acquires the sample, requiring navigation to Bennu's surface. A touch-and-go sample acquisition maneuver will result in the retrieval of at least 60 grams of regolith, and up to several kilograms. The flight activity concludes with a return cruise to Earth and delivery of the sample return capsule (SRC) for landing and sample recovery at the Utah Test and Training Range (UTTR).

Blood volume regulating hormones, fluid and electrolyte modifications during 21 and 198-day space flights (Altair-MIR 1993)

NASA Astrophysics Data System (ADS)

Vorobiev, D.; Maillet, A.; Fortrat, J. O.; Pastushkova, L.; Allevard, A. M.; Sigaudo, D.; Cartier, R.; Patricot, M.; Andre-Deshays, C.; Kotovskaya, A.; Grigoriev, A.; Gharib, C.; Gauquelin, G.

During the Altair MIR' 93 mission we studied several parameters involved in blood volume regulation. The experiment was done on two cosmonauts before (B-60, B-30), during (D6, D12, D18 for French and D7, D12, D17 for Russian) and after the flight (R+1, R+3 and R+7). Space flight durations were different for two cosmonauts: for the Russian the flight duration was 198 days and for the French 21 days. On board the MIR station only urinary (volume and electrolytes, atrial natriuretic peptide (ANP), cyclic guanosine monophosphate (cGMP) and catecholamines) and salivary (cGMP and cortisol) samples were collected, centrifuged and stored in freezer. Lithium was used as a tracer to know exactly the 24 h urine output (CNES urine collection Kit). Before and after flight, blood was drawn with an epicite needle and vacutainer system for hormonal assays (renin, antidiuretic hormone, cGMP, ANP and aldosterone) in two positions: after 30 min rest in upright seated position and after 90 min of supine position. Salivary samples were collected simultaneously. During flight a decrease of diuresis and ANP and an increase of osmolality were found. No modifications of hematocrit, but an increase of salivary cGMP and cortisol were also observed. The decrease of urinary ANP is in favor of hypovolemia as described in previous flights. The postflight examinations revealed changes in fluid-electrolyte metabolism which indicate a hypohydration status and a stimulation of hormonal system responsible for water and electrolyte retention in order to readapt to the normal gravity.

CDR De Winne takes Water Samples for analysis in the US Lab

NASA Image and Video Library

2009-10-20

ISS021-E-010368 (20 Oct. 2009) --- European Space Agency astronaut Frank De Winne (foreground), Expedition 21 commander, fills a bag with water in the Destiny laboratory of the International Space Station. NASA astronaut Jeffrey Williams, flight engineer, exercises on the Cycle Ergometer with Vibration Isolation System (CEVIS) at left.

Autonomous support for microorganism research in space

NASA Astrophysics Data System (ADS)

Fleet, M. L.; Smith, J. D.; Klaus, D. M.; Luttges, M. W.

1993-02-01

A preliminary design for performing on orbit, autonomous research on microorganisms and cultured cells/tissues is presented. The payload is designed to be compatible with the COMercial Experiment Transporter (COMET), an orbiter middeck locker interface and with Space Station Freedom. Uplink/downlink capabilities and sample return through controlled reentry are available for all carriers. Autonomous testing activities are preprogrammed with in-flight reprogrammability. Sensors for monitoring temperature, pH, light, gravity levels, vibrations, and radiation are provided for environmental regulation and experimental data collection. Additional data acquisition includes optical density measurement, microscopy, video, and film photography. On-board data storage capabilities are provided. A fluid transfer mechanism is utilized for inoculation, sampling, and nutrient replenishment of experiment cultures. In addition to payload design, research opportunities are explored to illustrate hardware versatility and function. The project is defined to provide biological data pertinent to extended duration crewed space flight including crew health issues and development of a Controlled Ecological Life Support System (CELSS). In addition, opportunities are opened for investigations leading to commercial applications of space, such as pharmaceutical development, modeling of terrestrial diseases, and material processing.

The beginning of Space Life Science in China exploration rockets for biological experiment during 1960's

NASA Astrophysics Data System (ADS)

Jiang, Peidong; Zhang, Jingxue

The first step of space biological experiment in China was a set of five exploration rockets launched during 1964 to 1966, by Shanghai Institute of Machine and Electricity, and Institute of Biophysics of The Chinese Academy of Sciences. Three T-7AS1rockets for rats, mice and other samples in a biological cabin were launched and recovered safely in July of 1964 and June of 1965. Two T-7AS2rockets for dog, rats, mice and other samples in a biological cabin were launched and recovered safely in July of 1966. Institute of Biophysics in charged of the general design of biological experiments, telemetry of physiological parameters, and selection and training of experiment animals. The samples on-board were: rats, mice, dogs, and test tubes with fruit fly, enzyme, bacteria, E. Coli., lysozyme, bacteriaphage, RNAase, DNAase, crystals of enzyme, etc. Physiological, biochemical, bacte-riological, immunological, genetic, histochemical studies had been conducted, in cellular and sub cellular level. The postures of rat and dog were monitored during flight and under weight-lessness. Physiological parameters of ECG, blood pressure, respiration rate, body temperature were recorded. A dog named"Xiao Bao"was flight in 1966 with video monitor, life support system and conditioned reflex equipment. It flighted for more than 20 minutes and about 70km high. After 40 years, the experimental data recorded of its four physiological parameters during the flight process was reviewed. The change of 4 parameters during various phase of total flight process were compared, analyzed and discussed.

Early Results and Spaceflight Implications of the SWAB Flight Experiment

NASA Technical Reports Server (NTRS)

Ott, C. Mark; Pierson, Duane L.

2007-01-01

Microbial monitoring of spacecraft environments provides key information in the assessment of infectious disease risk to the crew. Monitoring aboard the Mir space station and International Space Station (ISS) has provided a tremendous informational baseline to aid in determining the types and concentrations of microorganisms during a mission. Still, current microbial monitoring hardware utilizes culture-based methodology which may not detect many medically significant organisms, such as Legionella pneumophila. We hypothesize that evaluation of the ISS environment using non-culture-based technologies would reveal microorganisms not previously reported in spacecraft, allowing for a more complete health assessment. To achieve this goal, a spaceflight experiment, operationally designated as SWAB, was designed to evaluate the DNA from environmental samples collected from ISS and vehicles destined for ISS. Results from initial samples indicate that the sample collection and return procedures were successful. Analysis of these samples using denaturing gradient gel electrophoresis and targeted PCR primers for fungal contaminants is underway. The current results of SWAB and their implication for in-flight molecular analysis of environmental samples will be discussed.

View of Expedition 15 FE Anderson performing the ANITA Experiment in the Node 1

NASA Image and Video Library

2007-10-06

ISS015-E-32200 (6 Oct. 2007) --- Astronaut Clay Anderson, Expedition 15 flight engineer, uses an air sample pump and 2.5 liter gas sample bag to gather and analyze air samples for the Analyzing Interferometer for Ambient Air (ANITA) experiment in the Unity node of the International Space Station.

The Student Spaceflight Experiments Program: Access to the ISS for K-14 Students

NASA Astrophysics Data System (ADS)

Livengood, T. A.; Goldstein, J. J.; Hamel, S.; Manber, J.; Hulslander, M.

2013-12-01

The Student Spaceflight Experiments Program (SSEP) has flown 53 experiments to space, on behalf of students from middle school through community college, on 4 missions: each of the last 2 Space Shuttle flights, the first SpaceX demonstration flight to the International Space Station (ISS), and on SpaceX-1 to ISS. Two more missions to ISS have payloads flying in Fall 2013. SSEP plans 2 missions to the ISS per year for the foreseeable future, and is expanding the program to include 4-year undergraduate college students and home-schooled students. SSEP experiments have explored biological, chemical, and physical phenomena within self-contained enclosures developed by NanoRacks, currently in the form of MixStix Fluid Mixing Enclosures. 21,600 students participated in the initial 6 missions of SSEP, directly experiencing the entire lifecycle of space science experimentation through community-wide participation in SSEP, taking research from a nascent idea through developing competitive research proposals, down-selecting to three proposals from each participating community and further selection of a single proposal for flight, actual space flight, sample recovery, analysis, and reporting. The National Air and Space Museum has hosted 3 National Conferences for SSEP student teams to report results in keeping with the model of professional research. Student teams have unflinchingly reported on success, failure, and groundbased efforts to develop proposals for future flight opportunities. Community participation extends outside the sciences and the immediate proposal efforts to include design competitions for mission patches, which also fly to space. Student experimenters have rallied around successful proposal teams to support a successful experiment on behalf of the entire community. SSEP is a project of the National Center for Earth and Space Science Education enabled through NanoRacks LLC, working in partnership with NASA under a Space Act Agreement as part of the utilization of the International Space Station as a National Laboratory. 2012 Oct 06 - Astronaut Sunita Williams operating a Fluid Mixing Enclosure during SSEP Mission 2 on the International Space Station.

Wakata and Thirsk with MELFI in KIBO

NASA Image and Video Library

2009-06-15

ISS020-E-010021 (15 June 2009) --- Canadian Space Agency astronaut Robert Thirsk (left) and Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, both Expedition 20 flight engineers, prepare to put samples in the Minus Eighty Laboratory Freezer for ISS (MELFI) in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

Biodosimetry results from space flight Mir-18.

PubMed

Yang, T C; George, K; Johnson, A S; Durante, M; Fedorenko, B S

1997-11-01

Astronauts are classified as radiation workers due to the presence of ionizing radiation in space. For the assessment of health risks, physical dosimetry has been indispensable. However, the change of the location of dosimeters on the crew members, the variation in dose rate with location inside the spacecraft and the unknown biological effects of microgravity can introduce significant uncertainties in estimating exposure. To circumvent such uncertainty, a study on the cytogenetic effects of space radiation in human lymphocytes was proposed and conducted for Mir-18, a 115-day mission. This study used fluorescence in situ hybridization (FISH) with whole-chromosome painting probes to score chromosomal exchanges and the Giemsa staining method to determine the frequency of dicentrics. The growth kinetics of cells and sister chromatid exchanges (SCEs) were examined to ensure that chromosomal aberrations were scored in the first mitosis and were induced primarily by space radiation. Our results showed that the frequency of chromosomal aberrations increased significantly in postflight samples compared to samples drawn prior to flight, and that the frequency of SCEs was similar for both pre- and postflight samples. Based on a dose-response curve for preflight samples exposed to gamma rays, the absorbed dose received by crew members during the mission was estimated to be about 14.75 cSv. Because the absorbed dose measured by physical dosimeters is 5.2 cGy for the entire mission, the RBE is about 2.8.

Biodosimetry results from space flight Mir-18

NASA Technical Reports Server (NTRS)

Yang, T. C.; George, K.; Johnson, A. S.; Durante, M.; Fedorenko, B. S.

1997-01-01

Astronauts are classified as radiation workers due to the presence of ionizing radiation in space. For the assessment of health risks, physical dosimetry has been indispensable. However, the change of the location of dosimeters on the crew members, the variation in dose rate with location inside the spacecraft and the unknown biological effects of microgravity can introduce significant uncertainties in estimating exposure. To circumvent such uncertainty, a study on the cytogenetic effects of space radiation in human lymphocytes was proposed and conducted for Mir-18, a 115-day mission. This study used fluorescence in situ hybridization (FISH) with whole-chromosome painting probes to score chromosomal exchanges and the Giemsa staining method to determine the frequency of dicentrics. The growth kinetics of cells and sister chromatid exchanges (SCEs) were examined to ensure that chromosomal aberrations were scored in the first mitosis and were induced primarily by space radiation. Our results showed that the frequency of chromosomal aberrations increased significantly in postflight samples compared to samples drawn prior to flight, and that the frequency of SCEs was similar for both pre- and postflight samples. Based on a dose-response curve for preflight samples exposed to gamma rays, the absorbed dose received by crew members during the mission was estimated to be about 14.75 cSv. Because the absorbed dose measured by physical dosimeters is 5.2 cGy for the entire mission, the RBE is about 2.8.

Persistence of space radiation induced cytogenetic damage in the blood lymphocytes of astronauts.

PubMed

George, K; Chappell, L J; Cucinotta, F A

2010-08-14

Cytogenetic damage was assessed in blood lymphocytes from 16 astronauts before and after they participated in long-duration space missions of 3 months or more. The frequency of chromosome damage was measured by fluorescence in situ hybridization (FISH) chromosome painting before flight and at various intervals from a few days to many months after return from the mission. For all individuals, the frequency of chromosome exchanges measured within a month of return from space was higher than their preflight yield. However, some individuals showed a temporal decline in chromosome damage with time after flight. Statistical analysis using combined data for all astronauts indicated a significant overall decreasing trend in total chromosome exchanges with time after flight, although this trend was not seen for all astronauts and the yield of chromosome damage in some individuals actually increased with time after flight. The decreasing trend in total exchanges was slightly more significant when statistical analysis was restricted to data collected more than 220 days after return from flight. When analysis was restricted to data collected within 220 days of return from the mission there was no relationship between total exchanges and time. Translocation yields varied more between astronauts and there was only a slight non-significant decrease with time after flight that was similar for both later and earlier sampling times. Copyright (c) 2010. Published by Elsevier B.V.

Kotov works with samples from the Bioscience Experiment ASEPTIC during Joint Operations

NASA Image and Video Library

2010-02-19

ISS022-E-068638 (18 Feb. 2010) --- Russian cosmonaut Oleg Kotov, Expedition 22 flight engineer, works with samples from the bioscience experiment ASEPTIC (BTKh-39) in the new Russian Glavboks-S (Glovebox) located in the Poisk Mini-Research Module 2 (MRM2) of the International Space Station.

Kotov works with samples from the Bioscience Experiment ASEPTIC during Joint Operations

NASA Image and Video Library

2010-02-19

ISS022-E-068640 (18 Feb. 2010) --- Russian cosmonaut Oleg Kotov, Expedition 22 flight engineer, works with samples from the bioscience experiment ASEPTIC (BTKh-39) in the new Russian Glavboks-S (Glovebox) located in the Poisk Mini-Research Module 2 (MRM2) of the International Space Station.

Kotov works with samples from the Bioscience Experiment ASEPTIC during Joint Operations

NASA Image and Video Library

2010-02-19

ISS022-E-068645 (18 Feb. 2010) --- Russian cosmonaut Oleg Kotov, Expedition 22 flight engineer, works with samples from the bioscience experiment ASEPTIC (BTKh-39) in the new Russian Glavboks-S (Glovebox) located in the Poisk Mini-Research Module 2 (MRM2) of the International Space Station.

Nutrition and Repository: Insertion of Urine Sample into MELFI

NASA Image and Video Library

2009-04-18

ISS019-E-010170 (18 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

Coleman prepares to insert samples into the MELFI

NASA Image and Video Library

2010-12-27

ISS026-E-023768 (27 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, prepares to insert samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

Nutrition and Repository: Insertion of Urine Sample into MELFI

NASA Image and Video Library

2009-04-18

ISS019-E-010165 (18 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

Back to the future: the role of the ISS and future space stations in planetary exploration.

NASA Astrophysics Data System (ADS)

Muller, Christian; Moreau, Didier

2010-05-01

Space stations as stepping stones to planets appear already in the1954 Disney-von Braun anticipation TV show but the first study with a specific planetary scientific objective was the ANTEUS project of 1978. This station was an evolution of SPACELAB hardware and was designed to analyse Mars samples with better equipment than the laboratory of the VIKING landers. It would have played the role of the reception facility present in the current studies of Mars sample return, after analysis, the "safe" samples would have been returned to earth by the space shuttle. This study was followed by the flights of SPACELAB and MIR. Finally after 35 years of development, the International Space Station reaches its final configuration in 2010. Recent developments of the international agreement between the space agencies indicate a life extending to 2025, it is already part of the exploration programme as its crews prepare the long cruise flights and missions to the exploration targets. It is now time to envisage also the use of this stable 350 tons spacecraft for planetary and space sciences. Planetary telescopes are an obvious application; the present SOLAR payload on COLUMBUS is an opportunity to use the target pointing capabilities from the ISS. The current exposure facilities are also preparing future planetary protection procedures. Other applications have already been previously considered as experimental collision and impact studies in both space vacuum and microgravity. Future space stations at the Lagrange points could simultaneously combine unique observation platforms with an actual intermediate stepping stone to Mars.

KSC-2009-4925

NASA Image and Video Library

2009-08-28

CAPE CANAVERAL, Fla. – At NASA's Kennedy Space Center in Florida, space shuttle Discovery hurtles toward space on the STS-128 mission. Below the main engine nozzles are the blue mach diamonds, a formation of shock waves in the exhaust plume of an aerospace propulsion system Liftoff from Launch Pad 39A was on time at 11:59 p.m. EDT. The first launch attempt on Aug. 24 was postponed due to unfavorable weather conditions. The second attempt on Aug. 25 also was postponed due to an issue with a valve in space shuttle Discovery's main propulsion system. The STS-128 mission is the 30th International Space Station assembly flight and the 128th space shuttle flight. The 13-day mission will deliver more than 7 tons of supplies, science racks and equipment, as well as additional environmental hardware to sustain six crew members on the International Space Station. The equipment includes a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Photo credit: NASA/Tony Gray-Tom Farrar

Material Science

NASA Image and Video Library

2003-01-22

On Earth when scientists melt metals, bubbles that form in the molten material can rise to the surface, pop and disappear. In microgravity -- the near-weightless environment created as the International Space Station orbits Earth -- the lighter bubbles do not rise and disappear. Prior space experiments have shown that bubbles often become trapped in the final metal or crystal sample -similar to the bubbles trapped in this sample. In the solid, these bubbles, or porosity, are defects that diminish both the material's strength and usefulness. The Pore Formation and Mobility Investigation will melt samples of a transparent modeling material, succinonitrile and succinonitrile water mixtures, shown here in an ampoule being examined by Dr. Richard Grugel, the principal investigator for the experiment at NASA's Marshall Space Flight Center in Huntsville, Ala. As the samples are processed in space, Grugel will be able to observe how bubbles form in the samples and study their movements and interactions.

Analysis of Chromosomal Aberrations in the Blood Lymphocytes of Astronauts after Space Flight

NASA Technical Reports Server (NTRS)

George, K.; Kim, M. Y.; Elliott, T.; Cucinotta, F. A.

2007-01-01

It is a NASA requirement that biodosimetry analysis be performed on all US astronauts who participate in long duration missions of 3 months or more onboard the International Space Station. Cytogenetic analysis of blood lymphocytes is the most sensitive and reliable biodosimetry method available at present, especially if chromosome damage is assessed before as well as after space flight. Results provide a direct measurement of space radiation damage in vivo that takes into account individual radiosensitivity and considers the influence of microgravity and other stress conditions. We present data obtained from all twenty-five of the crewmembers who have participated in the biodosimetry program so far. The yield of chromosome exchanges, measured using fluorescence in situ hybridization (FISH) technique with chromosome painting probes, increased after space flight for all these individuals. In vivo dose was derived from frequencies of chromosome exchanges using preflight calibration curves of in vitro exposed cells from the same individual, and RBE was compared with individually measured physically absorbed dose and projected organ dose equivalents. Biodosimetry estimates using samples collected within a few weeks of return from space lie within the range expected from physical dosimetry. For some of these individuals chromosome aberrations were assessed again several months after their respective missions and a temporal decline in stable exchanges was observed in some cases, suggesting that translocations are unstable with time after whole body exposure to space radiation. This may indicate complications with the use of translocations for retrospective dose reconstruction. Data from one crewmember who has participated in two separate long duration space missions and has been followed up for over 10 years provides limited data on the effect of repeat flights and shows a possible adaptive response to space radiation exposure.

Nutritional Status Assessment (SMO 016E)

NASA Technical Reports Server (NTRS)

Smith, S. M.; Zwart, S. R.; Heer, M.; Coburn, S. P.; Booth, S. A.; Jones, J. A.; Lupton, J.

2007-01-01

It has not been possible to assess nutritional status of crew members on the ISS during flight because blood and urine could not be collected during ISS missions. Postflight observations of alterations in nutritional status for several nutrients are troubling, and we require the ability to monitor the status of these nutrients during flight to determine if there is a specific impetus or timeframe for these changes. In addition to the monitoring of crew nutritional status during flight, in-flight sample collection would allow better assessment of countermeasure effectiveness. SMO 016E is also designed to expand the current medical requirement for nutritional assessment (MR016L) to include additional normative markers for assessing crew health and countermeasure effectiveness. Additional markers of bone metabolism will be measured to better monitor bone health and the effectiveness of countermeasures to prevent bone resorption. New markers of oxidative damage will be measured to better assess the type of oxidative insults that occur during space flight. The array of nutritional assessment parameters will be expanded to include parameters that will allow us to better understand changes in folate and vitamin B6 status, and related cardiovascular risk factors during and after flight. Additionally, stress hormones and hormones that affect bone and muscle metabolism will also be measured. This additional assessment will allow us to better monitor the health of crew members and make more accurate recommendations for their rehabilitation. Several nutritional assessment parameters are altered at landing, but it is not known how long these changes persist. We extended the current protocol to include an additional postflight blood and urine sample collection 30 days after landing. Data are being collected before, during, and after flight. These data will provide a complete survey of how nutritional status and related systems are affected by space flight. Analyzing the data will help us to define nutritional requirements for long-duration missions. This expanded set of measurements will also aid in the identification of nutritional countermeasures to counteract, for example, the deleterious effects of microgravity on bone and muscle and the effects of space radiation.

KSC-2009-4839

NASA Image and Video Library

2009-08-24

CAPE CANAVERAL, Fla. – Xenon lights over Launch Pad 39A at NASA's Kennedy Space Center in Florida compete with the lightning strike seen to the left. Space shuttle Discovery is on the pad waiting for a scheduled liftoff on the STS-128 mission. Launch was scrubbed due to the weather conditions that violated the limitations for liftoff. Another launch attempt was scheduled for 1:10 a.m. Aug. 26. Discovery's 13-day mission will deliver more than 7 tons of supplies, science racks and equipment, as well as additional environmental hardware to sustain six crew members on the International Space Station. The equipment includes a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. The mission is the 128th in the Space Shuttle Program, the 37th flight of Discovery and the 30th station assembly flight. Photo credit: NASA/Ben Cooper

Creamer stows urine samples in MELFI

NASA Image and Video Library

2010-05-02

ISS023-E-030773 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Creamer stows urine samples in MELFI

NASA Image and Video Library

2010-05-02

ISS023-E-030771 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Creamer stows urine samples in MELFI

NASA Image and Video Library

2010-05-02

ISS023-E-030780 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Creamer stows urine samples in MELFI

NASA Image and Video Library

2010-05-02

ISS023-E-030772 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

FIR

NASA Image and Video Library

2013-09-16

ISS037-E-001115 (16 Sept. 2013) ---NASA astronaut Karen Nyberg, Expedition 37 flight engineer, works with test samples housed in the Light Microscopy Module (LMM) inside the Fluids Integrated Rack of the International Space Station’s Destiny laboratory.

Ion beam sputter-deposited thin film coatings for protection of spacecraft polymers in low Earth orbit

NASA Technical Reports Server (NTRS)

Banks, B. A.; Mirtich, M. J.; Rutledge, S. K.; Swec, D. M.; Nahra, H. K.

1985-01-01

Ion beam sputter-deposited thin films of Al2O3, SiO2, and a codeposited mixture of predominantly SiO2 with small amounts of a fluoropolymer were evaluated both in laboratory plasma ashing tests and in space on board shuttle flight STS-8 for effectiveness in preventing oxidation of polyimide Kapton. Measurements of mass loss and optical performance of coated and uncoated polyimide samples exposed to the low Earth orbital environment are presented. Optical techniques were used to measure loss rates of protective films exposed to atomic oxygen. Results of the analysis of the space flight exposed samples indicate that thin film metal oxide coatings are very effective in protecting the polyimide. Metal oxide coatings with a small amount of fluoropolymer codeposited have the additional benefit of great flexibility.

Ion beam sputter-deposited thin film coatings for protection of spacecraft polymers in low earth orbit

NASA Technical Reports Server (NTRS)

Banks, B. A.; Mirtich, M. J.; Rutledge, S. K.; Swec, D. M.; Nahra, H. K.

1985-01-01

Ion beam sputter-deposited thin films at Al2O3, SiO2, and a codeposited mixture of predominantly SiO2 with small amounts of fluoropolymer were evaluated both in laboratory plasma ashing tests and in space on board Shuttle flight STS-8 for effectiveness in preventing oxidation of polyimide Kapton. Measurements of mass loss and optical performance of coated and uncoated polyimide samples exposed to the low earth orbital environment are presented. Optical techniques were used to measure loss rates of protective films exposed to atomic oxygen. Results of the analysis of the space flight exposed samples indicate that thin film metal oxide coatings are very effective in protecting the polyimide. Metal oxide coatings with a small amount of fluoropolymer codeposited have the additional benefit of great flexibility.

NASA Biological Specimen Repository

NASA Technical Reports Server (NTRS)

Pietrzyk, Robert; McMonigal, K. A.; Sams, C. F.; Johnson, M. A.

2009-01-01

The NASA Biological Specimen Repository (NBSR) has been established to collect, process, annotate, store, and distribute specimens under the authority of the NASA/JSC Committee for the Protection of Human Subjects. The International Space Station (ISS) provides a platform to investigate the effects of microgravity on human physiology prior to lunar and exploration class missions. The NBSR is a secure controlled storage facility that is used to maintain biological specimens over extended periods of time, under well-controlled conditions, for future use in approved human spaceflight-related research protocols. The repository supports the Human Research Program, which is charged with identifying and investigating physiological changes that occur during human spaceflight, and developing and implementing effective countermeasures when necessary. The storage of crewmember samples from many different ISS flights in a single repository will be a valuable resource with which researchers can validate clinical hypotheses, study space-flight related changes, and investigate physiological markers All samples collected require written informed consent from each long duration crewmember. The NBSR collects blood and urine samples from all participating long duration ISS crewmembers. These biological samples are collected pre-flight at approximately 45 days prior to launch, during flight on flight days 15, 30, 60 120 and within 2 weeks of landing. Postflight sessions are conducted 3 and 30 days following landing. The number of inflight sessions is dependent on the duration of the mission. Operations began in 2007 and as of October 2009, 23 USOS crewmembers have completed or agreed to participate in this project. As currently planned, these human biological samples will be collected from crewmembers covering multiple ISS missions until the end of U.S. presence on the ISS or 2017. The NBSR will establish guidelines for sample distribution that are consistent with ethical principles, protection of crewmember confidentiality, prevailing laws and regulations, intellectual property policies, and consent form language. A NBSR Advisory Board composed of representatives of all participating agencies will be established to evaluate each request by an investigator for use of the samples to ensure the request reflects the mission of the NBSR.

Nanopore sequencing in microgravity

PubMed Central

McIntyre, Alexa B R; Rizzardi, Lindsay; Yu, Angela M; Alexander, Noah; Rosen, Gail L; Botkin, Douglas J; Stahl, Sarah E; John, Kristen K; Castro-Wallace, Sarah L; McGrath, Ken; Burton, Aaron S; Feinberg, Andrew P; Mason, Christopher E

2016-01-01

Rapid DNA sequencing and analysis has been a long-sought goal in remote research and point-of-care medicine. In microgravity, DNA sequencing can facilitate novel astrobiological research and close monitoring of crew health, but spaceflight places stringent restrictions on the mass and volume of instruments, crew operation time, and instrument functionality. The recent emergence of portable, nanopore-based tools with streamlined sample preparation protocols finally enables DNA sequencing on missions in microgravity. As a first step toward sequencing in space and aboard the International Space Station (ISS), we tested the Oxford Nanopore Technologies MinION during a parabolic flight to understand the effects of variable gravity on the instrument and data. In a successful proof-of-principle experiment, we found that the instrument generated DNA reads over the course of the flight, including the first ever sequenced in microgravity, and additional reads measured after the flight concluded its parabolas. Here we detail modifications to the sample-loading procedures to facilitate nanopore sequencing aboard the ISS and in other microgravity environments. We also evaluate existing analysis methods and outline two new approaches, the first based on a wave-fingerprint method and the second on entropy signal mapping. Computationally light analysis methods offer the potential for in situ species identification, but are limited by the error profiles (stays, skips, and mismatches) of older nanopore data. Higher accuracies attainable with modified sample processing methods and the latest version of flow cells will further enable the use of nanopore sequencers for diagnostics and research in space. PMID:28725742

International Space Station Air Quality Assessed According to Toxicologically-Grouped Compounds

NASA Technical Reports Server (NTRS)

James, John T.; Limero, Thomas F.; Beck, Steve; Cheng, Patti F.; deVera, Vanessa J.; Hand, Jennifer; Macatangay, Ariel

2010-01-01

Scores of compounds are found in the International Space Station (ISS) atmospheric samples that are returned to the Johnson Space Center Toxicology Laboratory for analysis. Spacecraft Maximum Allowable Concentrations (SMACs) are set with the view that each compound is present as if there were no other compounds present. In order to apply SMACs to the interpretation of the analytical data, the toxicologist must employ some method of combining the potential effects of the aggregate of compounds found in the atmospheric samples. The simplest approach is to assume that each quantifiable compound has the potential for some effect in proportion to the applicable SMAC, and then add all the proportions. This simple paradigm disregards the fact that most compounds have potential to adversely affect only a few physiological systems, and their effects would be independent rather than additive. An improved approach to dealing with exposure to mixtures is to add the proportions only for compounds that adversely affect the same physiological system. For example, toxicants that cause respiratory irritation are separated from those that cause neurotoxicity or cardio-toxicity. Herein we analyze ISS air quality data according to toxicological groups with a view that this could be used for understanding any crew symptoms occurring at the time of the sample acquisition. In addition, this approach could be useful in post-flight longitudinal surveys where the flight surgeon may need to identify post-flight, follow-up medical studies because of on-orbit exposures that target specific physiological systems.

International Space Station Air Quality Assessed According to Toxicologically-Grouped Compounds

NASA Technical Reports Server (NTRS)

James, John T.; Limero, Tom; DeVera, Vanessa; Cheng, Patti; Hand, Jennifer; Macatangay, Ariel; Beck, Steve

2009-01-01

Scores of compounds are found in the International Space Station (ISS) atmospheric samples that are returned to the Johnson Space Center Toxicology Laboratory for analysis. Spacecraft Maximum Allowable Concentrations (SMACs) are set with the view that each compound is present as if there were no other compounds present. In order to apply SMACs to the interpretation of the analytical data, the toxicologist must employ some method of combining the potential effects of the aggregate of compounds found in the atmospheric samples. The simplest approach is to assume that each quantifiable compound has the potential for some effect in proportion to the applicable SMAC, and then add all the proportions. This simple paradigm disregards the fact that most compounds have potential to adversely affect only a few physiological systems, and their effects would be independent rather than additive. An improved approach to dealing with exposure to mixtures is to add the proportions only for compounds that adversely affect the same physiological system. For example, toxicants that cause respiratory irritation are separated from those that cause neurotoxicity or cardio-toxicity. Herein we analyze ISS air quality data according to toxicological groups with a view that this could be used for understanding any crew symptoms occurring at the time of the sample. In addition, this approach could be useful in post-flight longitudinal surveys where the flight surgeon may need to identify post-flight, follow-up medical studies because of on-orbit exposures that target specific physiological systems.

Risk of Oxidative Damage to Bone from Increased Iron Stores During Space Flight

NASA Technical Reports Server (NTRS)

Zwart, S. R.; Smith, S. M.

2014-01-01

Iron stores are increased secondary to neocytolysis of red blood cells and a high dietary intake of iron during space flight. This raises concerns about the risk of excess iron causing oxidative damage in many tissues, including bone. Biomarkers of iron status, oxidative damage, and bone resorption during space flight were analyzed for 23 (16 M/7 F) International Space Station crewmembers as part of the Nutrition SMO project. Up to 5 in-flight blood samples and 24-h urine pools were collected over the course of the 4-6 month missions. Serum iron increased slightly during space flight and was decreased at landing (P < 0.0004). An increase in serum ferritin early in flight (217% in women and 68% in men, P < 0.0004), returning to preflight concentrations at landing, and a decrease in transferrin and transferrin receptors during flight indicated that a transient increase in iron stores occurred. No inflammatory response was observed during flight. The oxidative damage markers 8-hydroxy-2'-deoxyguanosine and prostaglandin F(sub 2(alpha)) were positively correlated (both P < 0.001) with serum ferritin. A greater area under the curve for ferritin during flight was correlated with greater changes in bone mineral density of several bone regions after flight (1). In a separate study (2), a ground-based investigation was conducted that examined the combined effects of radiation exposure and iron overload on sensitivity to radiation injury in several physiological systems in 12-wk male Sprague-Dawley rats. The rats were acclimated to an adequate iron diet (45 mg iron (ferric citrate)/kg diet) for 3 wk and then assigned to one of four groups: adequate iron (Fe) diet/no radiation, adequate Fe diet/ radiation, moderately high Fe diet (650 mg Fe (ferric citrate)/kg diet)/no radiation, and moderately high Fe diet/radiation. Animals remained on the assigned diet for 4 wk. Starting on day 14 of experimental diet treatment, animals were exposed to a fractionated dose (0.375 Gy) of Cs-137 every other day (3 Gy total dose). On day 29 (24 h after last radiation exposure), animals were euthanized. Oxidative stress markers in the liver, bone, eyes, and serum were assessed. There was evidence that the iron diet contributed to DNA damage as well as radiation exposure in the liver, eyes, and bone. Together, the results suggest that increased iron stores do constitute a risk factor for oxidative damage and bone resorption, during space flight and on Earth. Funded by the Human Health and Countermeasures Element of the NASA Human Research Program.

Hayabusa Re-Entry: Trajectory Analysis and Observation Mission Design

NASA Technical Reports Server (NTRS)

Cassell, Alan M.; Winter, Michael W.; Allen, Gary A.; Grinstead, Jay H.; Antimisiaris, Manny E.; Albers, James; Jenniskens, Peter

2011-01-01

On June 13th, 2010, the Hayabusa sample return capsule successfully re-entered Earth s atmosphere over the Woomera Prohibited Area in southern Australia in its quest to return fragments from the asteroid 1998 SF36 Itokawa . The sample return capsule entered at a super-orbital velocity of 12.04 km/sec (inertial), making it the second fastest human-made object to traverse the atmosphere. The NASA DC-8 airborne observatory was utilized as an instrument platform to record the luminous portion of the sample return capsule re-entry (60 sec) with a variety of on-board spectroscopic imaging instruments. The predicted sample return capsule s entry state information at 200 km altitude was propagated through the atmosphere to generate aerothermodynamic and trajectory data used for initial observation flight path design and planning. The DC- 8 flight path was designed by considering safety, optimal sample return capsule viewing geometry and aircraft capabilities in concert with key aerothermodynamic events along the predicted trajectory. Subsequent entry state vector updates provided by the Deep Space Network team at NASA s Jet Propulsion Laboratory were analyzed after the planned trajectory correction maneuvers to further refine the DC-8 observation flight path. Primary and alternate observation flight paths were generated during the mission planning phase which required coordination with Australian authorities for pre-mission approval. The final observation flight path was chosen based upon trade-offs between optimal viewing requirements, ground based observer locations (to facilitate post-flight trajectory reconstruction), predicted weather in the Woomera Prohibited Area and constraints imposed by flight path filing deadlines. To facilitate sample return capsule tracking by the instrument operators, a series of two racetrack flight path patterns were performed prior to the observation leg so the instruments could be pointed towards the region in the star background where the sample return capsule was expected to become visible. An overview of the design methodologies and trade-offs used in the Hayabusa re-entry observation campaign are presented.

Dichotomal effect of space flight-associated microgravity on stress-activated protein kinases in innate immunity

PubMed Central

Verhaar, Auke P.; Hoekstra, Elmer; Tjon, Angela S. W.; Utomo, Wesley K.; Deuring, J. Jasper; Bakker, Elvira R. M.; Muncan, Vanesa; Peppelenbosch, Maikel P.

2014-01-01

Space flight strongly moderates human immunity but is in general well tolerated. Elucidation of the mechanisms by which zero gravity interacts with human immunity may provide clues for developing rational avenues to deal with exaggerated immune responses, e.g. as in autoimmune disease. Using two sounding rockets and one manned Soyuz launch, the influence of space flight on immunological signal transduction provoked by lipopolysaccharide (LPS) stimulation was investigated in freshly isolated peripheral blood monocytes and was compared to samples obtained from on-board centrifuge-loaded 1 g controls. The effect of microgravity on immunological signal transduction is highly specific, since LPS dependent Jun-N-terminal kinase activation is impaired in the 0 g condition, while the corresponding LPS dependent activation of p38 MAP kinase remains unaffected. Thus our results identify Jun-N-terminal kinase as a relevant target in immunity for microgravity and support using Jun-N-terminal kinase specific inhibitors for combating autoimmune disease. PMID:24968806

Dichotomal effect of space flight-associated microgravity on stress-activated protein kinases in innate immunity.

PubMed

Verhaar, Auke P; Hoekstra, Elmer; Tjon, Angela S W; Utomo, Wesley K; Deuring, J Jasper; Bakker, Elvira R M; Muncan, Vanesa; Peppelenbosch, Maikel P

2014-06-27

Space flight strongly moderates human immunity but is in general well tolerated. Elucidation of the mechanisms by which zero gravity interacts with human immunity may provide clues for developing rational avenues to deal with exaggerated immune responses, e.g. as in autoimmune disease. Using two sounding rockets and one manned Soyuz launch, the influence of space flight on immunological signal transduction provoked by lipopolysaccharide (LPS) stimulation was investigated in freshly isolated peripheral blood monocytes and was compared to samples obtained from on-board centrifuge-loaded 1 g controls. The effect of microgravity on immunological signal transduction is highly specific, since LPS dependent Jun-N-terminal kinase activation is impaired in the 0 g condition, while the corresponding LPS dependent activation of p38 MAP kinase remains unaffected. Thus our results identify Jun-N-terminal kinase as a relevant target in immunity for microgravity and support using Jun-N-terminal kinase specific inhibitors for combating autoimmune disease.

Metabolic Cages for a Space Flight Model in the Rat

NASA Technical Reports Server (NTRS)

Harper, Jennifer S.; Mulenburg, Gerald M.; Evans, Juli; Navidi, Meena; Wolinsky, Ira; Arnaud, Sara B.

1994-01-01

A variety of space flight models are available to mimic the physiologic changes seen in the rat during weightlessness. The model reported by Wronski and Morey-Holton has been widely used by many investigators, in musculoskeletal physiologic studies especially, resulting in accumulation of an extensive database that enables scientists to mimic space flight effects in the 1-g environment of Earth. However, information on nutrition or gastrointestinal and renal function in this space flight model is limited by the difficulty in acquiring uncontaminated metabolic specimens for analysis. In the Holton system, a traction tape harness is applied to the tail, and the rat's hindquarters are elevated by attaching the harness to a pulley system. Weight-bearing hind limbs are unloaded, and there is a headward fluid shift. The tail-suspended rats are able to move freely about their cages on their forelimbs and tolerate this procedure with minimal signs of stress. The cage used in Holton's model is basically a clear acrylic box set on a plastic grid floor with the pulley and tail harness system attached to the open top of the cage. Food is available from a square food cup recessed into a corner of the floor. In this system, urine, feces, and spilled food fall through the grid floor onto absorbent paper beneath the cage and cannot be separated and recovered quantitatively for analysis in metabolic balance studies. Commercially available metabolic cages are generally cylindrical and have been used with a centrally located suspension apparatus in other space flight models. The large living area, three times as large as most metabolic cages, and the free range of motion unique to Holton's model, essential for musculoskeletal investigations, were sacrificed. Holton's cages can accommodate animals ranging in weight from 70 to 600 g. Although an alternative construction of Holton's cage has been reported, it does not permit collection of separate urine and fecal samples. We describe the modifications to Holton's food delivery system, cage base, and the addition of a separator system for the collection of urine and fecal samples for metabolic and nutrition studies in the tail suspension model.

78 FR 48542 - Agency Information Collection Activities: Requests for Comments; Clearance of Renewed Approval of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-08

... Flight Requirements for Crew and Space Flight Participants AGENCY: Federal Aviation Administration (FAA...-0720. Title: Human Space Flight Requirements for Crew and Space Flight Participants. Form Numbers... information collection. Background: The FAA has established requirements for human space flight of crew and...

78 FR 29425 - Agency Information Collection Activities: Requests for Comments; Clearance of Renewed Approval of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-20

... Flight Requirements for Crew and Space Flight Participants AGENCY: Federal Aviation Administration (FAA...-0720. Title: Human Space Flight Requirements for Crew and Space Flight Participants. Form Numbers... information collection. Background: The FAA has established requirements for human space flight of crew and...

Changes in Neutrophil Functions in Astronauts

NASA Technical Reports Server (NTRS)

Kaur, Indreshpal; Simons, Elizabeth R.; Castro, Victoria; Pierson, Duane L.

2002-01-01

Neutrophil functions (phagocytosis, oxidative burst, degranulation) and expression of surface markers involved in these functions were studied in 25 astronauts before and after 4 space shuttle missions. Space flight duration ranged from 5 to 11 days. Blood specimens were obtained 10 days before launch (preflight or L-10), immediately after landing (landing or R+0), and again at 3 days after landing (postflight or R+3). Blood samples were also collected from 9 healthy low-stressed subjects at 3 time points simulating a 10-day shuttle mission. The number of neutrophils increased at landing by 85 percent when compared to the preflight numbers. Neutrophil functions were studied in whole blood using flow cytometric methods. Phagocytosis of E.coli-FITC and oxidative burst capacity of the neutrophils following the 9 to 11 day missions were lower at all three sampling points than the mean values for control subjects. Phagocytosis and oxidative burst capacity of the astronauts was decreased even 10-days before space flight. Mission duration appears to be a factor in phagocytic and oxidative functions. In contrast, following the short-duration (5-days) mission, these functions were unchanged from control values. No consistent changes in degranulation were observed following either short or medium length space missions. The expression of CD16, CD32, CD11a, CD11b, CD11c, L-selectin and CD36 was measured and found to be variable. Specifically, CD16 and CD32 did not correlate with the changes in oxidative burst and phagocytosis. We can conclude from this study that the stresses associated with space flight can alter the important functions of neutrophils.

Evaluation of Space Power Materials Flown on the Passive Optical Sample Assembly

NASA Technical Reports Server (NTRS)

Jaworske, Donald A.; deGroh, Kim K.; Skowronski, Timothy J.; McCollum, Tim; Pippin, Gary; Bungay, Corey

1999-01-01

Evaluating the performance of materials on the exterior of spacecraft is of continuing interest, particularly in anticipation of those applications that will require a long duration in low Earth orbit. The Passive Optical Sample Assembly (POSA) experiment flown on the exterior of Mir as a risk mitigation experiment for the International Space Station was designed to better understand the interaction of materials with the low Earth orbit environment and to better understand the potential contamination threats that may be present in the vicinity of spacecraft. Deterioration in the optical performance of candidate space power materials due to the low Earth orbit environment, the contamination environment, or both, must be evaluated in order to propose measures to mitigate such deterioration. The thirty two samples of space power materials studied here include solar array blanket materials such as polyimide Kapton H and SiO(x) coated polyimide Kapton H, front surface aluminized sapphire, solar dynamic concentrator materials such as silver on spin coated polyimide and aluminum on spin coated polyimide, CV 1144 silicone, and the thermal control paint Z-93-P. The physical and optical properties that were evaluated prior to and after the POSA flight include mass, total, diffuse, and specular reflectance, solar absorptance, and infrared emittance. Additional post flight evaluation included scanning electron microscopy to observe surface features caused by the low Earth orbit environment and the contamination environment, and variable angle spectroscopic ellipsometry to identify contaminant type and thickness. This paper summarizes the results of pre- and post-flight measurements, identifies the mechanisms responsible for optical properties deterioration, and suggests improvements for the durability of materials in future missions.

Microgravity

NASA Image and Video Library

1999-07-27

A Memphis student working at the University of Alabama in Huntsville prepares samples for the first protein crystal growth experiments plarned to be performed aboard the International Space Station (ISS). The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)

Microgravity

NASA Image and Video Library

1999-07-27

Memphis students working at the University of Alabama in Huntsville prepare samples for the first protein crystal growth experiments plarned to be performed aboard the International Space Station (ISS). The proteins are placed in plastic tubing that is heat-sealed at the ends, then flash-frozen and preserved in a liquid nitrogen Dewar. Aboard the ISS, the nitrogen will be allowed to evaporated so the samples thaw and then slowly crystallize. They will be analyzed after return to Earth. Photo credit: NASA/Marshall Space Flight Center (MSFC)

Spacelab

NASA Image and Video Library

1981-01-01

Spacelab was a versatile laboratory carried in the Space Shuttle's cargo bay for special research flights. Its various elements could be combined to accommodate the many types of scientific research that could best be performed in space. Spacelab consisted of an enclosed, pressurized laboratory module and open U-shaped pallets located at the rear of the laboratory module. The laboratory module contained utilities, computers, work benches, and instrument racks to conduct scientific experiments in astronomy, physics, chemistry, biology, medicine, and engineering. Equipment, such as telescopes, anternas, and sensors, was mounted on pallets for direct exposure to space. A 1-meter (3.3-ft.) diameter aluminum tunnel, resembling a z-shaped tube, connected the crew compartment (mid deck) to the module. The reusable Spacelab allowed scientists to bring experiment samples back to Earth for post-flight analysis. Spacelab was a cooperative venture of the European Space Agency (ESA) and NASA. ESA was responsible for funding, developing, and building of Spacelab, while NASA was responsible for the launch and operational use of Spacelab. Spacelab missions were cooperative efforts between scientists and engineers from around the world. Teams from NASA centers, universities, private industry, government agencies and international space organizations designed the experiments. The Marshall Space Flight Center was NASA's lead center for monitoring the development of Spacelab and managing the program.

The Human in Space: Lesson from ISS

NASA Technical Reports Server (NTRS)

Sams, Clarence F.

2009-01-01

This viewgraph presentation reviews the lessons learned from manned space flight on the International Space Station. The contents include: 1) Overview of space flight effects on crewmembers; 2) General overview of immune system; 3) How does space flight alter immune system? 4) What factors associated with space flight inteact with crewmember immune function and impact health risks? 5) What is the current understanding of space flight effects on the immune system? and 6) Why should NASA be interested in immunology? Why is it significant?

Optical properties monitor: Experiment definition phase

NASA Technical Reports Server (NTRS)

Wilkes, Donald R.; Bennett, Jean M.; Hummer, Leigh L.; Chipman, Russell A.; Hadaway, James B.; Pezzaniti, Larry

1990-01-01

The stability of materials used in the space environment will continue to be a limiting technology for space missions. The Optical Properties Monitor (OPM) Experiment provides a comprehensive space research program to study the effects of the space environment (both natural and induced) on optical, thermal and space power materials. The OPM Experiment was selected for definition under the NASA/OAST In-Space Technology Experiment Program. The results of the OPM Definition Phase are presented. The OPM experiment will expose selected materials to the space environment and measure the effects with in-space optical measurements. In-space measurements include total hemispherical reflectance total integrated scatter and VUV reflectance/transmittance. The in-space measurements will be augmented with extensive pre- and post-flight sample measurements to determine other optical, mechanical, electrical, chemical or surface effects of space exposure. Environmental monitors will provide the amount and time history of the sample exposure to solar irradiation, atomic oxygen and molecular contamination.

Optical properties monitor: Experiment definition phase

NASA Technical Reports Server (NTRS)

Wilkes, Donald R.; Bennett, Jean M.; Hummer, Leigh L.; Chipman, Russell A.; Hadaway, James B.; Pezzaniti, Larry

1989-01-01

The stability of materials used in the space environment will continue to be a limiting technology for space missions. The Optical Properties Monitor (OPM) Experiment provides a comprehensive space research program to study the effects of the space environment-both natural and induced-on optical, thermal and space power materials. The OPM Experiment was selected for definition under the NASA/OAST In-Space Technology Experiment Program. The results of the OPM Definition Phase are presented. The OPM Experiment will expose selected materials to the space environment and measure the effects with in-space optical measurements. In-space measurements include total hemispherical reflectance total integrated scatter and VUV reflectance/transmittance. The in-space measurements will be augmented with extensive pre- and post-flight sample measurements to determine other optical, mechanical, electrical, chemical or surface effects of space exposure. Environmental monitors will provide the amount and time history of the sample exposure to solar irradiation, atomic oxygen and molecular contamination.

Nutrition in space

NASA Technical Reports Server (NTRS)

Smith, S. M.; Davis-Street, J.; Rice, B. L.; Lane, H. W.

1997-01-01

The authors review studies conducted to define nutritional requirements for astronauts during space flight and to assess nutrition before, during, and after space flight. Topics include space food systems, research and limitations on spacecraft, physiological adaptation to weightlessness, energy requirements, dietary intake during space flight, bone demineralization, gastrointestinal function, blood volume, and nutrition requirements for space flight. Benefits of space-related nutrition research are highlighted.

Cosmonauts' haemostasis system status before and after space flights

NASA Astrophysics Data System (ADS)

Kuzichkin, Dmitry; Markin, Andrey; Morukov, Boris

Introduction. It is known that cosmonauts expose themselves to psychophysical effort in different phases of space flights as well as in pre- and post-flight period. Stress affects different body systems functioning changes including haemostasis system. It is shown that adrenalin directly activates XII coagulation cascade factor [McKay D. G., Latour I. G., Parrish M. N.,1970], initiating intrinsic clotting pathway and affects fibrinogen concentration increase in plasma [Zubairov D. M., 1978]. A post-flight increase in the fibrinogen concentration was revealed with its drop up to the pre-flight level within rehabilitation period [T. Peter Stein, Margaret D., 2006]. Stress agents influence on haemostasis system is physiologically determined and directed to body preparation before probable blood loss. One can consider this process as a function of intrinsic clotting pathway. But in case of blood loss absence the preliminary permanent coagulation activation can lead to appearance of thrombosis risk. Purpose. The purpose was to study haemostasis system main components functional activity features before and after space flights. Methods. In the citrated plasma of astronauts who performed short-term (10 to 11 days) or long-term (196 to 199 days) the following values were determined: activated partial thrombin time (APTT); prothrombin time; prothrombin index; international normalized ratio; thrombin time (TT); activity of enzymes influencing the function of proteins involved in the formation and lysis of a clot such as antithrombin III, protein C, plasminogen, antiplasmin; content of fibrinogen, as well as intermediate products of formation and degradation of fibrin such as D-dimer, soluble fibrin-monomer complexes (SFMC). Sampling of biomaterial was perfomed 30 to 45 days prior to the flight, during the 1st day of the post flight period (all the examined persons), and in the 7th and 14th day (long-term flights member only) Results. In pre-flight period cosmonauts’ APTT indices was increased as compared with general population physiological norms. During the 1st day after long- and short-term flights a tendency for activation of coagulation system along inner and terminal pathways emerged (APTT, TT shortening, an increase in the SFMC concentration). After short-term space flights a tendency for activation of fibrin forming (an increase in the fibrin concentration) was evidenced, and, as a compensatory factor, for activation of fibrinolysis (an increase in fibrynolytic activity and D-dimer concentration). On the contrary, after long-term space flights, a tendency for fibrinolysys decline was observed (fibrinolytic activity and D-dimer concentration decreased at this the fibrinogen concentration remained virtually constant relative to the background level). During the 14th day of the post-flight period normalization of all studied parameters was observed. Discussion. After space flights a tendency for activation of haemostasis procoagulant component is observed. However, during short-term space flights compensatory systems become activated, which may be connected with developing of stress reactions of adaptation to weightlessness conditions and post-flight re-adaptation to ground conditions, while after long-term spaceflights the compensatory effect of fibrinolysis is not pronounced, possibly, due to metabolic process intensity reduction developing during long-duration stay in weightlessness conditions [Grigoriev A.I., Kaplansky A.S., Popova I.A., 1992]. Probably the relatively inactivated cosmonauts’ intrinsic pathway coagulation in pre-flight period (prolonged APTT) is one of the prerequisites of the high resistance to stress factors influence. Plausible this status of intrinsic pathway subject to consequent activation by adrenalin promotes body protection against thrombophilic tendency.

Post-Flight Evaluation of PICA and PICA-X - Comparisons of the Stardust SRC and Space-X Dragon 1 Forebody Heatshield Materials

NASA Technical Reports Server (NTRS)

Stackpoole, M.; Kao, D.; Qu, V.; Gonzales, G.

2013-01-01

Phenolic Impregnated Carbon Ablator (PICA) was developed at NASA Ames Research Center. As a thermal protection material, PICA has the advantages of being able to withstand high heat fluxes with a relatively low density. This ablative material was used as the forebody heat shield material for the Stardust sample return capsule, which re-entered the Earths atmosphere in 2006. Based on PICA, SpaceX developed a variant, PICA-X, and used it as the heat shield material for its Dragon spacecraft, which successfully orbited the Earth and re-entered the atmosphere during the COTS Demo Flight 1 in 2010. Post-flight analysis was previously performed on the Stardust PICA heat shield material. Similarly, a near-stagnation core was obtained from the post-flight Dragon 1 heat shield, which was retrieved from the Pacific Ocean. Materials testing and analyses were performed on the core to evaluate its ablation performance and post-flight properties. Comparisons between PICA and PICA-X are made where applicable. Stardust and Dragon offer rare opportunities to evaluate materials post-flight - this data is beneficial in understanding material performance and also improves modeling capabilities.

KSC-03PD-1526

NASA Technical Reports Server (NTRS)

2003-01-01

KENNEDY SPACE CENTER, FLA. Reporters at the dedication ceremony of a NASA hangar at the San Jose, Costa Rica, airport observe the WB-57f takeoff for its sixth Costa Rican flight. KSC and NASA researchers are testing the Aircraft-based Volcanic Emission Mass Spectrometer (AVEMS) that determines the presence and concentration of various chemicals. It is being tested in flights over the Turrialba volcano in Costa Rica, and in the crater, sampling and analyzing fresh volcanic gases in their natural chemical state. The AVEMS system has been developed for use in the Space Shuttle program, to detect toxic gas leaks and emissions in the Shuttles aft compartment and the crew compartment.

The growth of solar radiated yeast

NASA Technical Reports Server (NTRS)

Kraft, Tyrone

1995-01-01

This researcher plans to determine if solar radiation affects the growth of yeast. The irradiated yeast was obtained from a sample exposed in space during a Space Shuttle flight of September 9-20, 1994. Further, the control groups were held at: (1) Goddard Space Flight Center (GSFC) in Greenbelt, Maryland; and (2) South Dakota School of Mines and Technology. The procedure used was based on the fact that yeast is most often used in consumable baked goods. Therefore, the yeast was incorporated into a basic Betty Crocker bread recipe. Data was collected by placing measured amounts of dough into sample containers with fifteen minute growth in height measurements collected and recorded. This researcher assumed the viability of yeast to be relative to its ability to produce carbon dioxide gas and cause the dough to rise. As all ingredients and surroundings were equal, this researcher assumed the yeast will produce the only significant difference in data collected. This researcher noted the approximate use date on all sample packages to be prior to arrival and experiment date. All dates equal, it was then assumed each would act in a similar manner of response. This assumption will allow for equally correct data collection.

The growth of solar radiated yeast

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kraft, T.

This researcher plans to determine if solar radiation affects the growth of yeast. The irradiated yeast was obtained from a sample exposed in space during a Space Shuttle flight of September 9-20, 1994. Further, the control groups were held at: (1) Goddard Space Flight Center (GSFC) in Greenbelt, Maryland; and (2) South Dakota School of Mines and Technology. The procedure used was based on the fact that yeast is most often used in consumable baked goods. Therefore, the yeast was incorporated into a basic Betty Crocker bread recipe. Data was collected by placing measured amounts of dough into sample containersmore » with fifteen minute growth in height measurements collected and recorded. This researcher assumed the viability of yeast to be relative to its ability to produce carbon dioxide gas and cause the dough to rise. As all ingredients and surroundings were equal, this researcher assumed the yeast will produce the only significant difference in data collected. This researcher noted the approximate use date on all sample packages to be prior to arrival and experiment date. All dates equal, it was then assumed each would act in a similar manner of response. This assumption will allow for equally correct data collection.« less

Mass loss of shuttle space suit orthofabric under simulated ionospheric atomic oxygen bombardment

NASA Technical Reports Server (NTRS)

Miller, W. L.

1985-01-01

Many polymeric materials used for thermal protection and insulation on spacecraft degrade significantly under prolonged bombardment by ionospheric atomic oxygen. The covering fabric of the multilayered shuttle space suit is composed of a loose weave of GORE-TEX fibers, Nomex and Kevlar-29, which are all polymeric materials. The complete evaluation of suit fabric degradation from ionospheric atomic oxygen is of importance in reevaluating suit lifetime and inspection procedures. The mass loss and visible physical changes of each test sample was determined. Kapton control samples and data from previous asher and flight tests were used to scale the results to reflect ionospheric conditions at about 220 km altitude. It is predicted that the orthofabric loses mass in the ionosphere at a rate of about 66% of the original orthofabric mass/yr. The outer layer of the two-layer orthofabric test samples shows few easily visible signs of degradation, even when observed at 440X. It is concluded that the orthofabric could suffer significant loss of performance after much less than a year of total exposure time, while the degradation might be undetectable in post flight visual examinations of space suits.

Outgassing and dimensional changes of polymer matrix composites in space

NASA Technical Reports Server (NTRS)

Tennyson, R. C.; Matthews, R.

1993-01-01

A thermal-vacuum outgassing model and test protocol for predicting outgassing times and dimensional changes for polymer matrix composites is described. Experimental results derived from a 'control' sample are used to provide the basis for analytical predictions to compare with the outgassing response of Long Duration Exposure Facility (LDEF) flight samples.

Automated biowaste sampling system urine subsystem operating model, part 1

NASA Technical Reports Server (NTRS)

Fogal, G. L.; Mangialardi, J. K.; Rosen, F.

1973-01-01

The urine subsystem automatically provides for the collection, volume sensing, and sampling of urine from six subjects during space flight. Verification of the subsystem design was a primary objective of the current effort which was accomplished thru the detail design, fabrication, and verification testing of an operating model of the subsystem.

Post-Flight Characterization of Samples for the MISSE-7 Spacesuit Fabric Exposure Experiment

NASA Technical Reports Server (NTRS)

Gaier, James R.; Waters, Deborah L.; Jaworski, Donald A.; McCue, Terry R.; Folz, Angela; Baldwin, Sammantha; Clark, Gregory W.; Batman, Brittany; Bruce, John

2012-01-01

Six samples of pristine and dust-abraded outer layer spacesuit fabrics were included in the Materials International Space Station Experiment-7, in which they were exposed to the wake side low Earth orbit environment (LEO) on the International Space Station (ISS) for 18 months in order to determine whether abrasion by lunar dust increases radiation degradation. The fabric samples were characterized using optical microscopy, field emission scanning electron microscopy, and tensile testing before and after exposure on the ISS. Comparison of pre- and post-flight characterizations showed that wake side LEO environment darkened and reddened all six fabrics, increasing their integrated solar absorptance by 7 to 38 percent. There was a decrease in the ultimate tensile strength and elongation to failure of lunar dust abraded Apollo spacesuit fibers by a factor of four and increased the elastic modulus by a factor of two. The severity of the degradation of the fabric samples over this short exposure time demonstrates the necessity to find ways to prevent or mitigate radiation damage to spacesuits when planning extended missions to the Moon.

BUNDLE: A Novel Furnace for Performing Controlled Directional Solidification Experiments in a Microgravity Environment

NASA Technical Reports Server (NTRS)

Carrasquillo, Edgar J.; Griffin, Mark R.; Hammond, Monica S.; Johnson, Martin L.; Grugel, R. N.

2000-01-01

NASA Marshall Space Flight Center has developed a novel directional solidification furnace prototype for processing metals and alloys experiments in a microgravity environment. The BUNDLE (Bridgman Unidirectional Dendrite in Liquid Experiment) furnace is intended to accommodate the science requirements of Flight Definition Principle Investigators studying cellular/dendritic growth in aluminum and lead alloys at processing temperatures up to 1200 C. The furnace implements a number of innovative features to achieve high thermal gradients and quench rates in a low-power, light-weight design. These include a pyrolytic boron nitride/graphite composite heating element surrounded by layers of self-supporting refractory metal shielding, and a graphite fiber enhanced cold zone allowing high levels of heat extraction from the sample crucible. Novel to the BUNDLE design is an in-situ helium gas quench capability that ensures rapid freezing of the solidifying region (mushy zone) of the metal sample within the furnace without sample disturbance prior to quenching; this is a stringent requirement for subsequent analysis and understanding of microstructural development. The experiment hardware concept features multiple furnaces that may be "bundled" together so many samples, currently up to eight, can be processed at one time. The design of BUNDLE is flexible enough to be implemented on the Shuttle and Space Station in a number of locations (SpaceHab, Express Rack, MPESS, ISPR, etc). BUNDLE prototype furnaces have directionally solidified and quenched 1cm diameter lead - 5.8 weight percent antimony and aluminum - 4 weight percent copper alloys. Quenching of the mushy zone, as recorded by in-situ thermocouples, occurred on the order of 0.5 seconds or less, a rate within the PI's requirements. Subsequent metallographic examination revealed the solidified microstructure to be, as expected, unidirectional. Both the dendrite tips and the eutectic reaction were planar in nature indicating uniform axial heat flow. Delineation between the growing dendrites and eutectic structure with the "quenched-in" liquid was sharp, attesting to the efficacy of the helium quench. BUNDLE's conception, development, capability, and adaptability are presented (in view of Flight PI's needs and science requirements) through viewgraphs depicting actual hardware, generated thermal analysis, and micrographs prepared from BUNDLE processed, flight-like samples.

BUNDLE - A Novel Furnace for Performing Controlled Directional Solidification Experiments in a Microgravity Environment

NASA Technical Reports Server (NTRS)

Carrasquillo, Edgar J.; Griffin, Mark R.; Hammond, Monica S.; Johnson, Martin L.; Grugel, R. N.

2001-01-01

NASA Marshall Space Flight Center has developed a novel directional solidification furnace prototype for processing metals and alloys experiments in a microgravity environment. The BUNDLE (Bridgman Unidirectional Dendrite in Liquid Experiment) furnace is intended to accommodate the science requirements of Flight Definition Principle Investigators studying cellular/dendritic growth in aluminum and lead alloys at processing temperatures up to 1200 C. The furnace implements a number of innovative features to achieve high thermal gradients and quench rates in a low-power, light-weight design. These include a pyrolytic boron nitride/graphite composite heating element surrounded by layers of self-supporting refractory metal shielding, and a graphite fiber enhanced cold zone allowing high levels of heat extraction from the sample crucible. Novel to the BUNDLE design is an in-situ helium gas quench capability that ensures rapid freezing of the solidifying region (mushy zone) of the metal sample within the furnace without sample disturbance prior to quenching; this is a stringent requirement for subsequent analysis and understanding of microstructural development. The experiment hardware concept features multiple furnaces that may be "bundled" together so many samples, currently up to eight, can be processed at one time. The design of BUNDLE is flexible enough to be implemented on the Shuttle and Space Station in a number of locations (SpaceHab, Express Rack, MPESS, ISPR, etc). BUNDLE prototype furnaces have directionally solidified and quenched 1cm diameter lead - 5.8 weight percent antimony and aluminum - 4 weight percent copper alloys. Quenching of the mushy zone, as recorded by in-situ thermocouples, occurred on the order of 0.5 seconds or less, a rate within the PI's requirements. Subsequent metallographic examination revealed the solidified microstructure to be, as expected, unidirectional. Both the dendrite tips and the eutectic reaction were planar in nature indicating uniform axial heat flow. Delineation between the growing dendrites and eutectic structure with the "quenched-in" liquid was sharp, attesting to the efficacy of the helium quench. BUNDLE's conception, development, capability, and adaptability are presented (in view of Flight PI's needs and science requirements) through viewgraphs depicting actual hardware, generated thermal analysis, and micrographs prepared from BUNDLE processed, flight-like samples.

Role of Corticosteroids in Bone Loss During Space Flight

NASA Technical Reports Server (NTRS)

Wronski, Thomas J.; Halloran, Bernard P.; Miller, Scott C.

1998-01-01

The primary objective of this research project is to test the hypothesis that corticosteroids contribute to the adverse skeletal effects of space flight. To achieve this objective, serum corticosteroids, which are known to increase during space flight, must be maintained at normal physiologic levels in flight rats by a combination of adrenalectomy and corticosteroid supplementation via implanted hormone pellets. Bone analyses in these animals will then be compared to those of intact flight rats that, based on past experience, will undergo corticosteroid excess and bone loss during space flight. The results will reveal whether maintaining serum corticosteroids at physiologic levels in flight rats affects the skeletal abnormalities that normally develop during space flight. A positive response to this question would indicate that the bone loss and decreased bone formation associated with space flight are mediated, at least in part, by corticosteroid excess.

14 CFR 437.27 - Pre-flight and post-flight operations.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Pre-flight and post-flight operations. 437.27 Section 437.27 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION... Experimental Permit Operational Safety Documentation § 437.27 Pre-flight and post-flight operations. An...

14 CFR 437.27 - Pre-flight and post-flight operations.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Pre-flight and post-flight operations. 437.27 Section 437.27 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION... Experimental Permit Operational Safety Documentation § 437.27 Pre-flight and post-flight operations. An...

14 CFR 437.27 - Pre-flight and post-flight operations.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Pre-flight and post-flight operations. 437.27 Section 437.27 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION... Experimental Permit Operational Safety Documentation § 437.27 Pre-flight and post-flight operations. An...

14 CFR 437.27 - Pre-flight and post-flight operations.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Pre-flight and post-flight operations. 437.27 Section 437.27 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION... Experimental Permit Operational Safety Documentation § 437.27 Pre-flight and post-flight operations. An...

14 CFR 1214.115 - Standard services.

Code of Federal Regulations, 2010 CFR

2010-01-01

....115 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.... (d) A five-person flight crew: commander, pilot and three mission specialists. (e) Orbiter flight...

14 CFR 1214.115 - Standard services.

Code of Federal Regulations, 2013 CFR

2013-01-01

....115 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.... (d) A five-person flight crew: commander, pilot and three mission specialists. (e) Orbiter flight...

14 CFR 1214.115 - Standard services.

Code of Federal Regulations, 2012 CFR

2012-01-01

....115 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.... (d) A five-person flight crew: commander, pilot and three mission specialists. (e) Orbiter flight...

Use of phytochrome-dependent reaction in evaluating the effect of space flight factors on the plant organism

NASA Technical Reports Server (NTRS)

Shteyne, B. A.; Nevzgodina, L. V.; Miller, A. T.

1982-01-01

The effects of space flight factors on lettuce seeds aboard the Kosmos-936 and Kosmos-1129 satellites for 20 days were studied. The phytochrome dependent (PD) reaction of light sensitive seeds was a sensitive criterion for evaluating the biological effects of space flight factors. The PD reaction of air dry lettuce seeds was suppressed after space flight, especially if the seeds were exposed to open space during the flight. Space flight affects the physiological activity of both phytochrome forms, and both the phi sub 730 dependent reactions of lettuce seeds were suppressed.

Biological changes observed on rice and biological and genetic changes observed on tobacco [correction of tabacco] after space flight in the orbital station Salyut-7 (Biobloc III experiment).

PubMed

Bayonove, J; Burg, M; Delpoux, M; Mir, A

1984-01-01

Caryopses and isolated embryos from Rice (Oryza sativa L.) and Tobacco seeds (Nicotiana tabacum L. variety Xanthi) were studied in the Biobloc III container aboard the Soviet orbital space station SALYUT 7. The recovery from radiation damage under conditions of space flight was observed for rice caryopsis and embryos gamma irradiated (Co 60, 50 grays) prior to launch. There was a large decrease in the percentage of germinating seeds from the Tobacco strain tested when the seeds were exposed to heavy ions. Among the germinating plantlets there were few morphological anomalies. Furthermore, there was a significant greater amount of genetic change in those samples held in grids as compared to those in bags.

STS-114 Flight Day 5 Highlights

NASA Technical Reports Server (NTRS)

2005-01-01

Highlights of Day 5 of the STS-114 Return to Flight mission (Commander Eileen Collins, Pilot James Kelly, Mission Specialists Soichi Noguchi, Stephen Robinson, Andrew Thomas, Wendy Lawrence, and Charles Camarda) include video coverage of an extravehiclular activity (EVA) by Noguchi and Robinson. The other crew members of Discovery are seen on the flight deck and mid-deck helping the astronauts to suit-up. The objectives of the EVA are to test repair techniques on sample tiles in the shuttle's payload bay, to repair electrical equipment for a gyroscope on the International Space Station (ISS), and to install a replacement GPS antenna on the ISS. Noguchi and Robinson use a caulk gun and a putty knife to repair the sample tiles. The video contains several Earth views, including one of Baja California.

Genes In Space-5

NASA Image and Video Library

2018-04-13

iss055e020319 (April 13, 2018) --- Flight Engineer Ricky Arnold processes of samples inside the Miniature Polymerase Chain Reaction (miniPCR) for the Genes In Space-5 experiment. The research gathered from Genes in Space-5 may be valuable in the development of procedures to maintain astronaut health and prevent an increased risk of cancer on deep space missions. The investigation also provides a deeper understanding of the human immune system, while giving student researchers a direct connection to the space program and offering hands-on educational experiences on Earth and promoting involvement in STEM fields.

KSC-2009-5714

NASA Image and Video Library

2009-10-22

CAPE CANAVERAL, Fla. – In the Training Auditorium at NASA's Kennedy Space Center in Florida, STS-128 Commander Rick Sturckow, with the microphone, presents a plaque commemorating the mission to Center Director Bob Cabana. The presentation followed a program for Kennedy employees in which the crew talked about their experiences on the mission. At left is Mission Specialist John "Danny" Olivas; at right is Pilot Kevin Ford. More than 7 tons of supplies, science racks and equipment, as well as additional environmental hardware to sustain six crew members on the International Space Station were delivered to the International Space Station on the STS-128 mission. The equipment included a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. The mission was the 128th in the Space Shuttle Program, the 37th flight of Discovery and the 30th station assembly flight. Launch was Aug. 28, 2009. Photo credit: NASA/Kim Shiflett

Non-Topographic Space-Based Laser Remote Sensing

NASA Technical Reports Server (NTRS)

Yu, Anthony W.; Abshire, James B.; Riris, Haris; Purucker, Michael; Janches, Diego; Getty, Stephanie; Krainak, Michael A.; Stephen, Mark A.; Chen, Jeffrey R.; Li, Steve X.;

MISSE in the Materials and Processes Technical Information System (MAPTIS )

NASA Technical Reports Server (NTRS)

Burns, DeWitt; Finckenor, Miria; Henrie, Ben

2013-01-01

Materials International Space Station Experiment (MISSE) data is now being collected and distributed through the Materials and Processes Technical Information System (MAPTIS) at Marshall Space Flight Center in Huntsville, Alabama. MISSE data has been instrumental in many programs and continues to be an important source of data for the space community. To facilitate great access to the MISSE data the International Space Station (ISS) program office and MAPTIS are working to gather this data into a central location. The MISSE database contains information about materials, samples, and flights along with pictures, pdfs, excel files, word documents, and other files types. Major capabilities of the system are: access control, browsing, searching, reports, and record comparison. The search capabilities will search within any searchable files so even if the desired meta-data has not been associated data can still be retrieved. Other functionality will continue to be added to the MISSE database as the Athena Platform is expanded

Space Environment Effects on Stability of Medications Flown on Space Shuttles and the International Space Station (ISS)

NASA Technical Reports Server (NTRS)

Daniels, Vernie; Du, Jianping; Crady, Camille; Satterfield, Rick; Putcha, Lakshmi

2007-01-01

The purpose is to assess physical and chemical degradation of select pharmaceutical formulations from the Shuttle and ISS medical kits. Eleven pharmaceuticals dispensed as different dosage forms were selected based on their physical / chemical characteristics and susceptibility to environmental factors such as, temperature, humidity and light sensitivity. When available, ground-controls of the study medications with matching brand and lot numbers were used for comparison. Samples retrieved from flight were stored along with their matching controls in a temperature and humidity controlled environmental chamber. Temperature, humidity, and radiation data from the Shuttle and ISS were retrieved from onboard HOBO U12 Temp/RH Data Loggers, and from passive dosimeters. Physical and chemical analyses of the pharmaceuticals were conducted using validated United States Pharmacopeia (USP) methods. Results indicated degradation of 6 of the 11 formulations returned from space flights. Four formulations, Amoxicillin / Clavulanate, promethazine, sulfamethoxazole / trimethoprim, and ciprofloxacin tablets depicted discoloration after flight. Chemical content analyses using High or Ultra Performance Liquid Chromatography (HPLC / UPLC) methods revealed that dosage forms of Amoxicillin / Clavulanate, promethazine, sulfamethoxazole / trimethoprim, lidocaine, ciprofloxacin and mupirocin contained less than 95% of manufacturer s labeled claim of active drug compound. Shuttle and ISS environments affect stability and shelf life of certain mediations flown on these missions. Data analysis is in progress to examine the effect of specific space flight environmental factors on pharmaceutical stability. The degradation profiles generated from ground studies in analog environments will be useful in establishing predictive shelf-life profiles for medications intended for use during long-term space exploration missions.

ARC-2006-ACD06-0091-010

NASA Image and Video Library

2006-06-05

Space shuttle STS-121 FIT (Fly Immunity and Tumors) payload. Using Drosophila (fruit fly) to complete the experiments. Here we have sample preparation for post flight analysis of Drosophila (fruit fly) larva with Oana Marcu and Laura Higgins

Mars Rock Analysis Briefing

NASA Image and Video Library

2013-03-12

Paul Mahaffy (right), principal investigator for Curiosity's Sample Analysis at Mars (SAM) investigation at NASA's Goddard Space Flight Center in Maryland, demonstrates how the SAM instrument drilled and captured rock samples on the surface of Mars at a news conference, Tuesday, March 12, 2013 at NASA Headquarters in Washington. The analysis of the rock sample collected shows ancient Mars could have supported living microbes. Photo Credit: (NASA/Carla Cioffi)

Microgravity

NASA Image and Video Library

1998-10-10

Time-lapse exposure depicts Bioreactor rotation. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunourous tissues.

14 CFR § 1214.115 - Standard services.

Code of Federal Regulations, 2014 CFR

2014-01-01

...§ 1214.115 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.... (d) A five-person flight crew: commander, pilot and three mission specialists. (e) Orbiter flight...

KENNEDY SPACE CENTER, FLA. - Arturo Ramierez, Charles Curley and Duke Follistein, KSC and Costa Rican researchers, carry the hazardous gas detection system AVEMS to the central of the Turrialba volcano. The Aircraft-based Volcanic Emission Mass Spectrometer determines the presence and concentration of various chemicals. It is being tested in flights over the Turrialba volcano and in the crater, sampling and analyzing fresh volcanic gases in their natural chemical state. The AVEMS system has been developed for use in the Space Shuttle program, to detect toxic gas leaks and emissions in the Shuttle’s aft compartment and the crew compartment.

NASA Image and Video Library

2003-03-31

KENNEDY SPACE CENTER, FLA. - Arturo Ramierez, Charles Curley and Duke Follistein, KSC and Costa Rican researchers, carry the hazardous gas detection system AVEMS to the central of the Turrialba volcano. The Aircraft-based Volcanic Emission Mass Spectrometer determines the presence and concentration of various chemicals. It is being tested in flights over the Turrialba volcano and in the crater, sampling and analyzing fresh volcanic gases in their natural chemical state. The AVEMS system has been developed for use in the Space Shuttle program, to detect toxic gas leaks and emissions in the Shuttle’s aft compartment and the crew compartment.

Effects of the space flight environment on the immune system

NASA Technical Reports Server (NTRS)

Sonnenfeld, Gerald; Butel, Janet S.; Shearer, William T.

2003-01-01

Space flight conditions have a dramatic effect on a variety of physiologic functions of mammals, including muscle, bone, and neurovestibular function. Among the physiological functions that are affected when humans or animals are exposed to space flight conditions is the immune response. The focus of this review is on the function of the immune system in space flight conditions during actual space flights, as well as in models of space flight conditions on the earth. The experiments were carried out in tissue culture systems, in animal models, and in human subjects. The results indicate that space flight conditions alter cell-mediated immune responses, including lymphocyte proliferation and subset distribution, and cytokine production. The mechanism(s) of space flight-induced alterations in immune system function remain(s) to be established. It is likely, however, that multiple factors, including microgravity, stress, neuroendocrine factors, sleep disruption, and nutritional factors, are involved in altering certain functions of the immune system. Such alterations could lead to compromised defenses against infections and tumors.

Real-time monitoring of genetically modified Chlamydomonas reinhardtii during the Foton M3 space mission and ground irradiation experiment

NASA Astrophysics Data System (ADS)

Lambreva, Maya; Rea, Giuseppina; Antonacci, Amina; Serafini, Agnese; Damasso, Mario; Margonelli, Andrea; Johanningmeier, Udo; Bertalan, Ivo; Pezzotti, Gianni; Giardi, Maria Teresa

Long-term space exploration, colonization or habitation requires biological life support systems capable to cope with the deleterious space environment. The use of oxygenic photosynthetic microrganisms is an intriguing possibility mainly for food, O2 and nutraceutical compounds production. The critical points of utilizing plantsor algae-based life support systems are the microgravity and the ionizing radiation, which can influence the performance of these organisms. The aim of the present study was to assess the effects of space environment on the photosynthetic activity of various microrganisms and to select space stress-tolerant strains. Site-directed and random mutants of the unicellular green alga Chlamydomonas reinhardtii of Photosystem II D1 protein were used as a model system to test and select the amino acid substitutions capable to account for space stress tolerance. We focussed our studies also on the accumulation of the Photosystem II photoprotective carotenoids (the xantophylls violaxanthin, anteraxanthin and zeaxanthin), powerful antioxidants that epidemiological studies demonstrated to be human vision protectors. Metabolite profiling by quantitative HPLC methods revealed the organisms and the stress conditions capable to accumulate the highest pigment levels. In order to develop a project for a rationale metabolic engineering of algal secondary metabolites overproduction, we are performing expression analyses on the carotenoid biosynthetic pathway under physiological and mimicked space conditions. To identify the consequences of the space environment on the photosynthetic apparatus the changes in the Photosystem II efficiency were monitored in real time during the ESA-Russian Foton-M3 mission in September 2007. For the space flight a high-tech, multicell fluorescence biosensor, Photo-II, was designed and built by the Centre for Advanced Research in Space Optics in collaboration with Kayser-Italy, Biosensor and DAS. Photo-II is an automatic device developed to measure the chlorophyll fluorescence and to provide a living conditions for 24 different algae strains. Twelve different C. reinhardtii strains were analytically selected and two replications for each strain were brought to space, among them, some mutants modified at the level of enzymes involved in the biosynthesis of xanthophylls. We analysed the hourly changes and the daily light/dark trend in the maximum quantum yield of PSII photochemistry as well as some physiological parameters that characterize the post-flight effect on algae viability and photosynthetic performance. The ground control experiments were performed following the same protocol for the sample preparation and the temperature recorded during the pre-flight, flight and post-flight phases. The space flight results in comparison to the ground simulations are discussed.

Autonomous support for microorganism research in space

NASA Technical Reports Server (NTRS)

Fleet, Mary L.; Miller, Mark S.; Shipley, Derek, E.; Smith, Jeff D.

1992-01-01

A preliminary design for performing on orbit, autonomous research on microorganisms and cultured cells/tissues is presented. An understanding of gravity and its effects on cells is crucial for space exploration as well as for terrestrial applications. The payload is designed to be compatible with the Commercial Experiment Transporter (COMET) launch vehicle, an orbiter middeck locker interface, and with Space Station Freedom. Uplink/downlink capabilities and sample return through controlled reentry are available for all carriers. Autonomous testing activities are preprogrammed with in-flight reprogrammability. Sensors for monitoring temperature, pH, light, gravity levels, vibrations, and radiation are provided for environmental regulation and experimental data collection. Additional experimental data acquisition includes optical density measurement, microscopy, video, and film photography. On-board full data storage capabilities are provided. A fluid transfer mechanism is utilized for inoculation, sampling, and nutrient replenishment of experiment cultures. In addition to payload design, representative experiments were developed to ensure scientific objectives remained compatible with hardware capabilities. The project is defined to provide biological data pertinent to extended duration crewed space flight including crew health issues and development of a Controlled Ecological Life Support System (CELSS). In addition, opportunities are opened for investigations leading to commercial applications of space, such as pharmaceutical development, modeling of terrestrial diseases, and material processing.

Sub-orbital commercial Human space flight and informed consent in the United States

NASA Astrophysics Data System (ADS)

Carminati, Maria-Vittoria « Giugi »; Griffith, Doug; Campbell, Mark R.

2013-12-01

Commercial space flight is expected to rapidly develop in the near future. This will begin with sub-orbital missions and then progress to orbital flights. In the United States, technical informed consent of space flight participants is required by the commercial space flight operator for regulatory purposes. Additionally, though not required by U.S. regulation, the aerospace medicine professional involved in the medical screening of both space flight participants and crewmembers will be asked to assist operators in obtaining medical informed consent for liability purposes. The various US federal and state regulations regarding informed consent for sub-orbital commercial space flight are evolving and are unfamiliar to most aerospace medical professionals and are reviewed and discussed.

Comparison of ground-based and space flight energy expenditure and water turnover in middle-aged healthy male US astronauts

NASA Technical Reports Server (NTRS)

Lane, H. W.; Gretebeck, R. J.; Schoeller, D. A.; Davis-Street, J.; Socki, R. A.; Gibson, E. K.

1997-01-01

Energy requirements during space flight are poorly defined because they depend on metabolic-balance studies, food disappearance, and dietary records. Water turnover has been estimated by balance methods only. The purpose of this study was to determine energy requirements and water turnover for short-term space flights (8-14 d). Subjects were 13 male astronauts aged 36-51 y with normal body mass indexes (BMIs). Total energy expenditure (TEE) was determined during both a ground-based period and space flight and compared with the World Health Organization (WHO) calculations of energy requirements and dietary intake. TEE was not different for the ground-based and the space-flight periods (12.40 +/- 2.83 and 11.70 +/- 1.89 MJ/d, respectively), and the WHO calculation using the moderate activity correction was a good predictor of TEE during space flight. During the ground-based period, energy intake and TEE did not differ, but during space flight energy intake was significantly lower than TEE; body weight was also less at landing than before flight. Water turnover was lower during space flight than during the ground-based period (2.7 +/- 0.6 compared with 3.8 +/- 0.5 L/d), probably because of lower fluid intakes and perspiration loss during flight. This study confirmed that the WHO calculation can be used for male crew members' energy requirements during short space flights.

Thermal Control Materials on MISSE-5 with Comparison to Earlier Flight Data

NASA Technical Reports Server (NTRS)

Finckenor, Miria; Zwiener, James M.; Pippin, Gary

2007-01-01

A variety of thermal control materials were flown on the Materials on International Space Station Experiment (MISSE)-5. Several types of beta cloth, as used in multi-layer insulation blankets, were flown, including samples from the same batch as used on the International Space Station. Two candidate sunshade materials for the James Webb Space Telescope were also exposed on MISSE-5. The white thermal control coating AZ93 was applied to Kapton instead of aluminum; this sample maintained good solar absorptance and did not indicate any significant level of contamination to the MISSE-5 experiment. Marker coatings maintained their color. Thermo-optical properties are discussed, along with comparable data from MISSE-2 and the Passive Optical Sample Assembly (POSA) - I experiments.

Time-of-flight radio location system

DOEpatents

McEwan, T.E.

1996-04-23

A bi-static radar configuration measures the direct time-of-flight of a transmitted RF pulse and is capable of measuring this time-of-flight with a jitter on the order of about one pico-second, or about 0.01 inch of free space distance for an electromagnetic pulse over a range of about one to ten feet. A transmitter transmits a sequence of electromagnetic pulses in response to a transmit timing signal, and a receiver samples the sequence of electromagnetic pulses with controlled timing in response to a receive timing signal, and generates a sample signal in response to the samples. A timing circuit supplies the transmit timing signal to the transmitter and supplies the receive timing signal to the receiver. The receive timing signal causes the receiver to sample the sequence of electromagnetic pulses such that the time between transmission of pulses in the sequence and sampling by the receiver sweeps over a range of delays. The receive timing signal sweeps over the range of delays in a sweep cycle such that pulses in the sequence are sampled at the pulse repetition rate, and with different delays in the range of delays to produce a sample signal representing magnitude of a received pulse in equivalent time. Automatic gain control circuitry in the receiver controls the magnitude of the equivalent time sample signal. A signal processor analyzes the sample signal to indicate the time-of-flight of the electromagnetic pulses in the sequence. 7 figs.

Time-of-flight radio location system

DOEpatents

McEwan, Thomas E.

1996-01-01

A bi-static radar configuration measures the direct time-of-flight of a transmitted RF pulse and is capable of measuring this time-of-flight with a jitter on the order of about one pico-second, or about 0.01 inch of free space distance for an electromagnetic pulse over a range of about one to ten feet. A transmitter transmits a sequence of electromagnetic pulses in response to a transmit timing signal, and a receiver samples the sequence of electromagnetic pulses with controlled timing in response to a receive timing signal, and generates a sample signal in response to the samples. A timing circuit supplies the transmit timing signal to the transmitter and supplies the receive timing signal to the receiver. The receive timing signal causes the receiver to sample the sequence of electromagnetic pulses such that the time between transmission of pulses in the sequence and sampling by the receiver sweeps over a range of delays. The receive timing signal sweeps over the range of delays in a sweep cycle such that pulses in the sequence are sampled at the pulse repetition rate, and with different delays in the range of delays to produce a sample signal representing magnitude of a received pulse in equivalent time. Automatic gain control circuitry in the receiver controls the magnitude of the equivalent time sample signal. A signal processor analyzes the sample signal to indicate the time-of-flight of the electromagnetic pulses in the sequence.

Wavefront Sensing with the Fine Guidance Sensor for James Webb Space Telescope

NASA Technical Reports Server (NTRS)

Smith, J. Scott; Aronstein, David; Dean, Bruce H.; Howard,Joe; Shiri, Ron

2008-01-01

An analysis is presented that utilizes the Fine Guidance Sensor (FGS) for focal-plane wavefront sensing (WFS) for the James Webb Space Telescope (JWST). WFS with FGS increases the number of wavefront measurements taken in field of the telescope, but has many challenges over the other JWST instruments that make it unique, such as; less sampling of the Point Spread Function (PSF), a smaller diversity-defocus range, a smaller image detector size, and a polychromatic object or source. Additionally, presented is an analysis of sampling for wavefront sensing. Results are shown based on simulations of flight and the cryogenic optical testing at NASA Johnson Space Center.

Long range planning for the development of space flight emergency systems.

NASA Technical Reports Server (NTRS)

Bolger, P. H.; Childs, C. W.

1972-01-01

The importance of long-range planning for space flight emergency systems is pointed out. Factors in emergency systems planning are considered, giving attention to some of the mission classes which have to be taken into account. Examples of the hazards in space flight include fire, decompression, mechanical structure failures, radiation, collision, and meteoroid penetration. The criteria for rescue vehicles are examined together with aspects regarding the conduction of rescue missions. Future space flight programs are discussed, taking into consideration low earth orbit space stations, geosynchronous orbit space stations, lunar operations, manned planetary missions, future space flight vehicles, the space shuttle, special purpose space vehicles, and a reusable nuclear shuttle.

Young PHD's in Human Space Flight

NASA Technical Reports Server (NTRS)

Wilson, Eleanor

2002-01-01

The Cooperating Hampton Roads Organizations for Minorities in Engineering (CHROME) in cooperation with the NASA Office of Space Flight, Human Exploration and Development of Space Enterprise sponsored a summer institute, Young PHD#s (Persons Having Dreams) in Human Space Flight. This 3-day institute used the curriculum of a workshop designed for space professionals, 'Human Space Flight-Analysis and Design: An Integrated, Systematic Approach.' The content was tailored to a high school audience. This institute seeks to stimulate the interest of pre-college students in space flight and motivate them to pursue further experiences in this field. Additionally, this institute will serve as a pilot model for a pre- collegiate training program that can be replicated throughout the country. The institute was complemented with a trip to the Goddard Space Flight Center.

Proteomic Analysis of Hair Follicles

NASA Astrophysics Data System (ADS)

Ishioka, Noriaki; Terada, Masahiro; Yamada, Shin; Seki, Masaya; Takahashi, Rika; Majima, Hideyuki J.; Higashibata, Akira; Mukai, Chiaki

2013-02-01

Hair root cells actively divide in a hair follicle, and they sensitively reflect physical conditions. By analyzing the human hair, we can know stress levels on the human body and metabolic conditions caused by microgravity environment and cosmic radiation. The Japan Aerospace Exploration Agency (JAXA) has initiated a human research study to investigate the effects of long-term space flight on gene expression and mineral metabolism by analyzing hair samples of astronauts who stayed in the International Space Station (ISS) for 6 months. During long-term flights, the physiological effects on astronauts include muscle atrophy and bone calcium loss. Furthermore, radiation and psychological effects are important issue to consider. Therefore, an understanding of the effects of the space environment is important for developing countermeasures against the effects experienced by astronauts. In this experiment, we identify functionally important target proteins that integrate transcriptome, mineral metabolism and proteome profiles from human hair. To compare the protein expression data with the gene expression data from hair roots, we developed the protein processing method. We extracted the protein from five strands of hair using ISOGEN reagents. Then, these extracted proteins were analyzed by LC-MS/MS. These collected profiles will give us useful physiological information to examine the effect of space flight.

KSC-2009-4917

NASA Image and Video Library

2009-08-28

CAPE CANAVERAL, Fla. – Space shuttle Discovery rises majestically from Launch Pad 39A at NASA's Kennedy Space Center in Florida as it heads for space on the STS-128 mission. Below the main engine nozzles are the blue mach diamonds, a formation of shock waves in the exhaust plume of an aerospace propulsion system. Liftoff from Launch Pad 39A was on time at 11:59 p.m. EDT. The first launch attempt on Aug. 24 was postponed due to unfavorable weather conditions. The second attempt on Aug. 25 also was postponed due to an issue with a valve in space shuttle Discovery's main propulsion system. The STS-128 mission is the 30th International Space Station assembly flight and the 128th space shuttle flight. The 13-day mission will deliver more than 7 tons of supplies, science racks and equipment, as well as additional environmental hardware to sustain six crew members on the International Space Station. The equipment includes a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Photo credit: NASA/Rusty Backer-George Roberts

Results of the First US Manned Orbital Space Flight

NASA Technical Reports Server (NTRS)

1962-01-01

The results of the first United States manned orbital space flight conducted on February 20, 1962 are presented. The prelaunch activities, spacecraft description, flight operations, flight data, and postflight analyses presented form a continuation of the information previously published for the two United States manned suborbital space flights conducted on May 5, 1961, and July 21, 1961, respectively, by the National Aeronautics and Space Administration.

76 FR 24836 - Regulatory Approach for Commercial Orbital Human Spaceflight

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-03

... regulating commercial human space flight. In December 2006, the FAA issued human space flight regulations in... space flight participants until December 23, 2012, or until a design feature or operating practice has... or serious injury, to crew or space flight participants during a licensed or permitted commercial...

Expedition 28 Crew Members remove samples from the JPM MELFI

NASA Image and Video Library

2011-07-08

ISS028-E-014918 (8 July 2011) --- NASA astronauts Ron Garan (left) and Mike Fossum, both Expedition 28 flight engineers, remove samples from the General Laboratory Active Cryogenic ISS Experiment Refrigerator (GLACIER) and insert in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Expedition 28 Crew Members remove samples from the JPM MELFI

NASA Image and Video Library

2011-07-08

ISS028-E-014916 (8 July 2011) --- NASA astronauts Ron Garan (left) and Mike Fossum, both Expedition 28 flight engineers, remove samples from the General Laboratory Active Cryogenic ISS Experiment Refrigerator (GLACIER) and insert in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Energy requirements for space flight

NASA Technical Reports Server (NTRS)

Lane, Helen W.

1992-01-01

Both the United States and the Soviet Union perform human space research. This paper reviews data available on energy metabolism in the microgravity of space flight. The level of energy utilization in space seems to be similar to that on earth, as does energy availability. However, despite adequate intake of energy and protein and in-flight exercise, lean body mass was catabolized, as indicated by negative nitrogen balance. Metabolic studies during simulated microgravity (bed rest) and true microgravity in flight have shown changes in blood glucose, fatty acids and insulin concentrations, suggesting that energy metabolism may be altered during space flight. Future research should focus on the interactions of lean body mass, diet and exercise in space, and their roles in energy metabolism during space flight.

Aerosol measurements over the Pacific Ocean in support of the IR aerosol backscatter program

NASA Technical Reports Server (NTRS)

Prospero, Joseph M.; Savoie, Dennis L.

1995-01-01

The major efforts under NASA contract NAG8-841 included: (1) final analyses of the samples collected during the first GLOBE survey flight that occurred in November 1989 and collections and analysis of aerosol samples during the second GLOBE survey flight in May and June 1990. During the first GLOBE survey flight, daily samples were collected at four stations (Midway, Rarotonga, American Samoa, and Norfolk Island) throughout the month of November 1989. Weekly samples were collected at Shemya, Alaska, and at Karamea, New Zealand. During the second GLOBE survey flight, daily samples were collected at Midway, Oahu, American Samoa, Rarotonga, and Norfolk Island; weekly samples were collected at Shemya. These samples were all analyzed for sodium (sea-salt), chloride, nitrate, sulfate, and methanesulfonate at the University of Miami and for aluminum at the University of Rhode Island (under a subcontract). (2) Samples continued to be collected on a weekly basis at all stations during the periods between and after the survey flights. These weekly samples were also analyzed at the University of Miami for the suite of water-soluble species. (3) In August 1990, the results obtained from the above studies were submitted to the appropriate personnel at NASA Marshall Space Flight Center to become part of the GLOBE data base for comparison with data from instruments used aboard the aircraft. In addition, the data will be compared with data previously obtained at these stations as part of the Sea-Air Exchange (SEAREX) Program. This comparison will provide valuable information on the representativeness of the periods in terms of the longer term aerosol climatology over the Pacific Ocean. (4) Several publications have been written using data from this grant. The data will continue to be used in the future as part of a continuing investigation of the long-term trends and interannual variations in aerosol species concentrations over the Pacific Ocean.

Wakata prepares for Surface Sample Kit (SSK) Collection/Incubation

NASA Image and Video Library

2009-04-29

ISS019-E-012393 (29 April 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 19/20 flight engineer, is pictured near a Microbial Air Sampler floating freely in the Kibo laboratory of the International Space Station.

CASKAD. Manual Mixing in Bioreactor

NASA Image and Video Library

2013-10-05

ISS037-E-005692 (5 Oct. 2013) --- Russian cosmonaut Sergey Ryazanskiy, Expedition 37 flight engineer, prepares to manually mix samples in a Bioreactor for the CASKAD experiment in the Poisk Mini-Research Module 2 (MRM2) of the International Space Station.

CASKAD. Manual Mixing in Bioreactor

NASA Image and Video Library

2013-10-05

ISS037-E-005694 (5 Oct. 2013) --- Russian cosmonaut Sergey Ryazanskiy, Expedition 37 flight engineer, prepares to manually mix samples in a Bioreactor for the CASKAD experiment in the Poisk Mini-Research Module 2 (MRM2) of the International Space Station.

Microgravity

NASA Image and Video Library

1997-03-11

This photo shows the access through the internal airlock on the Microgravity Science Glovebox (MSG) being developed by the European Space Agency (ESA) and NASA for use aboard the International Space Station (ISS). The airlock will allow the insertion or removal of equipment and samples without opening the working volume of the glovebox. Scientists will use the MSG to carry out multidisciplinary studies in combustion science, fluid physics and materials science. The MSG is managed by NASA's Marshall Space Flight Center (MSFC). Photo Credit: NASA/MSFC

Space Flight. Teacher Resources.

ERIC Educational Resources Information Center

2001

This teacher's guide contains information, lesson plans, and diverse student learning activities focusing on space flight. The guide is divided into seven sections: (1) "Drawing Activities" (Future Flight; Space Fun; Mission: Draw); (2) "Geography" (Space Places); (3) "History" (Space and Time); (4)…

Progress on an external occulter testbed at flight Fresnel numbers

NASA Astrophysics Data System (ADS)

Kim, Yunjong; Sirbu, Dan; Galvin, Michael; Kasdin, N. Jeremy; Vanderbei, Robert J.

2016-01-01

An external occulter is a spacecraft flown along the line-of-sight of a space telescope to suppress starlight and enable high-contrast direct imaging of exoplanets. Laboratory verification of occulter designs is necessary to validate the optical models used to design and predict occulter performance. At Princeton, we have designed and built a testbed that allows verification of scaled occulter designs whose suppressed shadow is mathematically identical to that of space occulters. The occulter testbed uses 78 m optical propagation distance to realize the flight Fresnel numbers. We will use an etched silicon mask as the occulter. The occulter is illuminated by a diverging laser beam to reduce the aberrations from the optics before the occulter. Here, we present first light result of a sample design operating at a flight Fresnel number and the mechanical design of the testbed. We compare the experimental results with simulations that predict the ultimate contrast performance.

On the Utilization of In-flight Radiation-induced Performance Data and Anomaly Resolution of Commercial Off the Shelf (COTS) Electronics

NASA Technical Reports Server (NTRS)

LeBel, Kenneth A.; Poivey, Christian; Barth, Janet L.

2003-01-01

This viewgraph presentation presents an overview of the use of in-flight science data to review the radiation effects on commercial off the shelf (COTS) electronics used in recent spacecraft missions. The authors review the hazards that the space radiation environment pose for spacecraft electronics. They specifically discuss long term effects such as total ionizing dose (TID) and short term effects like single particle events (SEE). The advantages of using COTS electronics despite not being radiation hardened are mentioned. The reasons cite for tracking in-flight performance of COTS electronics include: anomaly resolution, validate ground tests and environmental predictions and provide lessons for future designers. Sample radiation impacts of science data from the following missions are analyzed: SOHO/LASCO 3 Coronograph, Microwave Anisotrophy Probe, Hubble Space Telescope and Chandra X-Ray Observatory.

The International Space Station Urine Monitoring System (UMS)

NASA Technical Reports Server (NTRS)

Feeback, Daniel L.; Cibuzar, Branelle R.; Milstead, Jeffery R.; Pietrzyk,, Robert A.; Clark, Mark S.F.

2009-01-01

A device capable of making in-flight volume measurements of single void urine samples, the Urine Monitoring System (UMS), was developed and flown on seven U.S. Space Shuttle missions. This device provided volume data for each urine void from multiple crewmembers and allowed samples of each to be taken and returned to Earth for post-flight analysis. There were a number of design flaws in the original instrument including the presence of liquid carry-over producing invalid "actual" micturition volumes and cross-contamination between successive users from residual urine in "dead" spots". Additionally, high or low volume voids could not be accurately measured, the on-orbit calibration and nominal use sequence was time intensive, and the unit had to be returned and disassembled to retrieve the volume data. These problems have been resolved in a new version, the International Space Station (ISS) UMS, that has been designed to provide real-time in-flight volume data with accuracy and precision equivalent to measurements made on Earth and the ability to provide urine samples that are unadulterated by the device. Originally conceived to be interfaced with a U.S.-built Waste Collection System (WCS), the unit now has been modified to interface with the Russian-supplied Sanitary Hygiene Device (ASY). The ISS UMS provides significant advantages over the current method of collecting urine samples into Urine Collection Devices (UCDs), from which samples are removed and returned to Earth for analyses. A significant future advantage of the UMS is that it can provide an interface to analytical instrumentation that will allow real-time measurement of urine bioanalytes allowing monitoring of crewmember health status during flight and the ability to provide medical interventions based on the results of these measurements. Currently, the ISS UMS is scheduled to launch along with Node-3 on STS-130 (20A) in December 2009. UMS will be installed and scientific/functional verification completed prior to placing the instrument into operation. Samples collected during the verification sequence will be returned for analyses on STS-131 (19A) currently scheduled for launch in March 2010. The presence of a UMS on ISS will provide the capability to conduct additional collaborative human life science investigations among the ISS International Partners.

Preparation, testing and analysis of zinc diffusion samples, NASA Skylab experiment M-558

NASA Technical Reports Server (NTRS)

Braski, D. N.; Kobisk, E. H.; Odonnell, F. R.

1974-01-01

Transport mechanisms of zinc atoms in molten zinc were investigated by radiotracer techniques in unit and in near-zero gravity environments. Each melt in the Skylab flight experiments was maintained in a thermal gradient of 420 C to 790 C. Similar tests were performed in a unit gravity environment for comparison. After melting in the gradient furnace followed by a thermal soak period (the latter was used for flight samples only), the samples were cooled and analyzed for Zn-65 distribution. All samples melted in a unit gravity environment were found to have uniform Zn-65 distribution - no concentration gradient was observed even when the sample was brought rapidly to melting and then quenched. Space-melted samples, however, showed textbook distributions, obviously the result of diffusion. It was evident that convection phenomena were the dominant factors influencing zinc transport in unit gravity experiments, while diffusion was the dominant factor in near-zero gravity experiments.

14 CFR 91.109 - Flight instruction; Simulated instrument flight and certain flight tests.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Flight instruction; Simulated instrument flight and certain flight tests. 91.109 Section 91.109 Aeronautics and Space FEDERAL AVIATION... OPERATING AND FLIGHT RULES Flight Rules General § 91.109 Flight instruction; Simulated instrument flight and...

14 CFR 91.109 - Flight instruction; Simulated instrument flight and certain flight tests.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Flight instruction; Simulated instrument flight and certain flight tests. 91.109 Section 91.109 Aeronautics and Space FEDERAL AVIATION... OPERATING AND FLIGHT RULES Flight Rules General § 91.109 Flight instruction; Simulated instrument flight and...

14 CFR 91.109 - Flight instruction; Simulated instrument flight and certain flight tests.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Flight instruction; Simulated instrument flight and certain flight tests. 91.109 Section 91.109 Aeronautics and Space FEDERAL AVIATION... OPERATING AND FLIGHT RULES Flight Rules General § 91.109 Flight instruction; Simulated instrument flight and...

14 CFR 91.109 - Flight instruction; Simulated instrument flight and certain flight tests.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Flight instruction; Simulated instrument flight and certain flight tests. 91.109 Section 91.109 Aeronautics and Space FEDERAL AVIATION... OPERATING AND FLIGHT RULES Flight Rules General § 91.109 Flight instruction; Simulated instrument flight and...

14 CFR 91.109 - Flight instruction; Simulated instrument flight and certain flight tests.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Flight instruction; Simulated instrument flight and certain flight tests. 91.109 Section 91.109 Aeronautics and Space FEDERAL AVIATION... OPERATING AND FLIGHT RULES Flight Rules General § 91.109 Flight instruction; Simulated instrument flight and...

Properties of electrophoretic fractions of human embryonic kidney cells separated on space shuttle flight STS-8

NASA Technical Reports Server (NTRS)

Morrison, D. R.; Lewis, M. L.; Barlow, G. H.; Todd, P. W.; Kunze, M. E.; Sarnoff, B. E.; Li, Z. K.

1985-01-01

Suspensions of cultured primary human embryonic kidney cells were subjected to continuous flow electrophoresis on Space Shuttle flight STS-8. The objectives of the experiments were to obtain electrophoretically separated fractions of the original cell populations and to test these fractions for the amount and kind of urokinase (a kidney plasminogen activator that is used medically for digesting blood clots), the morphologies of cells in the individual fractions, and their cellular electrophoretic mobilities after separation and subsequent proliferation. Individual fractions were successfully cultured after return from orbit, and they were found to differ substantially from one another and from the starting sample with respect to all of these properties.

Space acceleration measurement system description and operations on the First Spacelab Life Sciences Mission

NASA Technical Reports Server (NTRS)

Delombard, Richard; Finley, Brian D.

1991-01-01

The Space Acceleration Measurement System (SAMS) project and flight units are briefly described. The SAMS operations during the STS-40 mission are summarized, and a preliminary look at some of the acceleration data from that mission are provided. The background and rationale for the SAMS project is described to better illustrate its goals. The functions and capabilities of each SAMS flight unit are first explained, then the STS-40 mission, the SAMS's function for that mission, and the preparation of the SAMS are described. Observations about the SAMS operations during the first SAMS mission are then discussed. Some sample data are presented illustrating several aspects of the mission's microgravity environment.

Thermal control surfaces on the MSFC LDEF experiments

NASA Technical Reports Server (NTRS)

Wilkes, Donald R.; Whitaker, Ann F.; Zwiener, James M.; Linton, Roger C.; Shular, David; Peters, Palmer N.; Gregory, John C.

1992-01-01

There were five Marshall Space Flight Center (MSFC) experiments on the LDEF. Each of those experiments carried thermal control surfaces either as test samples or as operational surfaces. These materials experienced varying degrees of mechanical and optical damage. Some materials were virtually unchanged by the extended exposure while others suffered extensive degradation. The synergistic effects due to the constituents of the space environment are evident in the diversity of these material changes. The sample complement for the MSFC experiments is described along with results of the continuing analyses efforts.

Astronauts Working in Spacelab

NASA Technical Reports Server (NTRS)

1999-01-01

This Quick Time movie captures astronaut Jan Davis and her fellow crew members working in the Spacelab, a versatile laboratory carried in the Space Shuttle's cargo bay for special research flights. Its various elements can be combined to accommodate the many types of scientific research that can best be performed in space. Spacelab consisted of an enclosed, pressurized laboratory module and open U-shaped pallets located at the rear of the laboratory module. The laboratory module contained utilities, computers, work benches, and instrument racks to conduct scientific experiments in astronomy, physics, chemistry, biology, medicine, and engineering. Equipment, such as telescopes, antennas, and sensors, is mounted on pallets for direct exposure to space. A 1-meter (3.3-ft.) diameter aluminum tunnel, resembling a z-shaped tube, connected the crew compartment (mid deck) to the module. The reusable Spacelab allowed scientists to bring experiment samples back to Earth for post-flight analysis. Spacelab was a cooperative venture of the European Space Agency (ESA) and NASA. ESA was responsible for funding, developing, and building Spacelab, while NASA was responsible for the launch and operational use of Spacelab. Spacelab missions were cooperative efforts between scientists and engineers from around the world. Teams from NASA centers, universities, private industry, government agencies and international space organizations designed the experiments. The Marshall Space Flight Center was NASA's lead center for monitoring the development of Spacelab and managing the program.

14 CFR 415.8 - Human space flight.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Human space flight. 415.8 Section 415.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH LICENSE General § 415.8 Human space flight. To obtain a launch license, an...

14 CFR 415.8 - Human space flight.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Human space flight. 415.8 Section 415.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH LICENSE General § 415.8 Human space flight. To obtain a launch license, an...

14 CFR 415.8 - Human space flight.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Human space flight. 415.8 Section 415.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH LICENSE General § 415.8 Human space flight. To obtain a launch license, an...

14 CFR 415.8 - Human space flight.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Human space flight. 415.8 Section 415.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH LICENSE General § 415.8 Human space flight. To obtain a launch license, an...

14 CFR 415.8 - Human space flight.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Human space flight. 415.8 Section 415.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH LICENSE General § 415.8 Human space flight. To obtain a launch license, an...

14 CFR 1214.101 - Eligibility for flight of a non-U.S. government reimbursable payload on the Space Shuttle.

Code of Federal Regulations, 2012 CFR

2012-01-01

.... government reimbursable payload on the Space Shuttle. 1214.101 Section 1214.101 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle... non-U.S. government reimbursable payload on the Space Shuttle. To be eligible for flight on the Space...

14 CFR 1214.101 - Eligibility for flight of a non-U.S. government reimbursable payload on the Space Shuttle.

Code of Federal Regulations, 2013 CFR

2013-01-01

.... government reimbursable payload on the Space Shuttle. 1214.101 Section 1214.101 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle... non-U.S. government reimbursable payload on the Space Shuttle. To be eligible for flight on the Space...

14 CFR 1214.101 - Eligibility for flight of a non-U.S. government reimbursable payload on the Space Shuttle.

Code of Federal Regulations, 2011 CFR

2011-01-01

.... government reimbursable payload on the Space Shuttle. 1214.101 Section 1214.101 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle... non-U.S. government reimbursable payload on the Space Shuttle. To be eligible for flight on the Space...

The effect of space microgravity on the physiological activity of mammalian resident cardiac stem cells

NASA Astrophysics Data System (ADS)

Belostotskaya, Galina; Zakharov, Eugeny

Prolonged exposure to weightlessness during space flights is known to cause depression of heart function in mammals. The decrease in heart weight and its remodeling under the influence of prolonged weightlessness (or space microgravity) is assumed to be due to both morphological changes of working cardiomyocytes and their progressive loss, as well as to possible depletion of resident cardiac stem cells (CSCs) population, or their inability to self-renewal and regeneration of muscle tissue under conditions of weightlessness. We have previously shown that the presence of different maturity clones formed by resident CSCs not only in culture but also in the mammalian myocardium can be used as an indicator of the regenerative activity of myocardial cells [Belostotskaya, et al., 2013: 2014]. In this study, we were interested to investigate whether the 30-day near-Earth space flight on the spacecraft BION-M1 affects the regenerative potential of resident CSCs. Immediately after landing of the spacecraft, we had examined the presence of resident c-kit+, Sca-1+ and Isl1+ CSCs and their development in suspension of freshly isolated myocardial cells of C57BL mice in comparison to controls. Cardiac cell suspension was obtained by enzymatic digestion of the heart [Belostotskaya and Golovanova, 2014]. Immunocytochemically stained preparations of fixed cells were analyzed with confocal microscope Leica TCS SP5 (Germany) in the Resource Center of St-Petersburg State University. CSCs were labeled with appropriate antibodies. CSCs differentiation into mature cardiomyocytes was verified using antibodies to Sarcomeric α-Actinin and Cardiac Troponin T. Antibodies to Connexin43 were used to detect cell-cell contacts. All antibodies were conjugated with Alexa fluorochromes (488, 532, 546, 568, 594 and/or 647 nm), according to Zenon-technology (Invitrogen). It has been shown that, under identical conditions of cell isolation, more complete digestion of heart muscle was observed in weightlessness-treated samples vs. controls. These findings correlated with reduced expression of Connexin43. Typical elongated cardiomyocytes, presenting as both individual cells and conglomerates, were present in the control samples, whereas the shortened and thickened individual cardiac myocytes prevailed in the samples subjected to space microgravity. Both control samples and microgravity-treated samples contained resident CSCs of all subtypes. Both individual CSCs and CSC-derived clones were present in the suspension of myocardial cells. However, the number of CSC-formed clones of different maturity was significantly higher in the samples subjected to space microgravity. Some clones comprised only small undifferentiated cells of one CSCs subtype, while the cells of the other clones expressed some of the specific cardiac antigens (α-Actinin and Troponin T) at varying rate. In addition, large α-actinin- and troponin T-positive individual cardiomyocytes with readily discernible sarcomeric structure still expressing the original CSC antigens were also identified. The data obtained suggest that prolonged space microgravity exposure during space flight causes significant structural changes in the mammalian myocardium which may affect cardiac contractile function. Weightlessness-induced loss in heart muscle weight is assumed to be compensated by an increase in the activity of resident CSCs, which form new cardiomyocytes proliferating and differentiating inside the clones. The authors express their gratitude to the staff of Institute of Biomedical Problems of the Russian Academy of Sciences and Company "Progress" for the preparation of experimental animals for the biosatellite flight. The study was in part supported by grants from BION-M1 Project and Program of Presidium of Russian Academy of Sciences “Fundamental Sciences for Medicine” (2013).

Mission Preparation Program for Exobiological Experiments in Earth Orbit

NASA Astrophysics Data System (ADS)

Panitz, Corinna; Reitz, Guenther; Horneck, Gerda; Rabbow, Elke; Rettberg, Petra

The ESA facilities EXPOSE-R and EXPOSE-E on board of the the International Space Station ISS provide the technology for exposing chemical and biological samples in a controlled manner to outer space parameters, such as high vacuum, intense radiation of galactic and solar origin and microgravity. EXPOSE-E has been attached to the outer balcony of the European Columbus module of the ISS in Febraury 2008 and will stay for about 1 year in space, EXPOSE-R will be attached to the Russian Svezda module of the ISS in fall 2008. The EXPOSE facilities are a further step in the study of the Responses of Organisms to Space Environment (ROSE concortium). The results from the EXPOSE missions will give new insights into the survivability of terrestrial organisms in space and will contribute to the understanding of the organic chemistry processes in space, the biological adaptation strategies to extreme conditions, e.g. on early Earth and Mars, and the distribution of life beyond its planet of origin.To test the compatibility of the different biological and chemical systems and their adaptation to the opportunities and constraints of space conditions a profound ground support program has been developed. It resulted in several experiment verification tests EVTs and an experiment sequence test EST that were conducted in the carefully equipped and monitored planetary and space simulation facilities PSI of the Institute of Aerospace Medicine at DLR in Cologne, Germany. These ground based pre-flight studies allow the investigation of a much wider variety of samples and the selection of the most promising organisms for the flight experiment. The procedure and results of these EVT tests and EST will be presented. These results are an essential prerequisite for the success of the EXPOSE missions and have been done in parallel with the development and construction of the final hardware design of the facility. The results gained during the simulation experiments demonstrated mission preparation as a basic requirement for successful and significant results of every space flight experiment. Hence, the Mission preparation program that was performed in the context of the space missions EXPOSE-E and EXPOSE-R proofed the outstanding importance and accentuated need for ground based experiments.

Metabolic and Regulatory Systems in Space Flight

NASA Technical Reports Server (NTRS)

1997-01-01

In this session, Session JP2, the discussion focuses on the following topics: The Dynamics of Blood Biochemical Parameters in Cosmonauts During Long-Term Space Flights; Efficiency of Functional Loading Test for Investigations of Metabolic Responses to Weightlessness; Human Cellular Immunity and Space Flight; Cytokine Production and Head-Down Tilt Bed Rest; Plasma and Urine Amino Acids During Human Space Flight; and DNA Fingerprinting, Applications to Space Microbiology.

Continuous flow electrophoresis system experiments on shuttle flights STS-6 and STS-7

NASA Technical Reports Server (NTRS)

Snyder, Robert S.; Rhodes, Percy H.; Miller, Teresa Y.

1988-01-01

The development of a space continuous flow electrophoresis system (CFES) is discussed. The objectives of the experiment were: (1) to use a model sample material at a high concentration to evaluate the continuous flow electrophoresis process in the McDonnell Douglass CFES instrument and compare its separation resolution and sample throughput with related devices on Earth, and (2) to expand the basic knowledge of the limitations imposed by fluid flows and particle concentration effects on the electrophoresis process by careful design and evaluation of the space experiment. Hemoglobin and polysaccharide were selected as samples of concentration effects. The results from space show a large band spread of the high concentration of the single species of hemoglobin that was principally due to the mismatch of electrical conductivity between the sample and buffer.

Bacillus subtilis spores on artificial meteorites survive hypervelocity atmospheric entry: implications for Lithopanspermia.

PubMed

Fajardo-Cavazos, Patricia; Link, Lindsey; Melosh, H Jay; Nicholson, Wayne L

2005-12-01

An important but untested aspect of the lithopanspermia hypothesis is that microbes situated on or within meteorites could survive hypervelocity entry from space through Earth's atmosphere. The use of high-altitude sounding rockets to test this notion was explored. Granite samples permeated with spores of Bacillus subtilis strain WN511 were attached to the exterior telemetry module of a sounding rocket and launched from White Sands Missile Range, New Mexico into space, reaching maximum atmospheric entry velocity of 1.2 km/s. Maximum recorded temperature during the flight was measured at 145 degrees C. The surfaces of the post-flight granite samples were swabbed and tested for recovery and survival of WN511 spores, using genetic markers and the unique DNA fingerprint of WN511 as recovery criteria. Spore survivors were isolated at high frequency, ranging from 1.2% to 4.4% compared with ground controls, from all surfaces except the forward-facing surface. Sporulation-defective mutants were noted among the spaceflight survivors at high frequency (4%). These experiments constitute the first report of spore survival to hypervelocity atmospheric transit, and indicate that sounding rocket flights can be used to model the high-speed atmospheric entry of bacteria-laden artificial meteorites.

Bacillus subtilis Spores on Artificial Meteorites Survive Hypervelocity Atmospheric Entry: Implications for Lithopanspermia

NASA Astrophysics Data System (ADS)

Fajardo-Cavazos, Patricia; Link, Lindsey; Melosh, H. Jay; Nicholson, Wayne L.

2005-12-01

An important but untested aspect of the lithopanspermia hypothesis is that microbes situated on or within meteorites could survive hypervelocity entry from space through Earth's atmosphere. The use of high-altitude sounding rockets to test this notion was explored. Granite samples permeated with spores of Bacillus subtilis strain WN511 were attached to the exterior telemetry module of a sounding rocket and launched from White Sands Missile Range, New Mexico into space, reaching maximum atmospheric entry velocity of 1.2 km/s. Maximum recorded temperature during the flight was measured at 145°C. The surfaces of the post-flight granite samples were swabbed and tested for recovery and survival of WN511 spores, using genetic markers and the unique DNA fingerprint of WN511 as recovery criteria. Spore survivors were isolated at high frequency, ranging from 1.2% to 4.4% compared with ground controls, from all surfaces except the forward-facing surface. Sporulation-defective mutants were noted among the spaceflight survivors at high frequency (4%). These experiments constitute the first report of spore survival to hypervelocity atmospheric transit, and indicate that sounding rocket flights can be used to model the high-speed atmospheric entry of bacteria-laden artificial meteorites.

Results from the Joint US/Russian Sensory-Motor Investigations

NASA Technical Reports Server (NTRS)

1997-01-01

In this session, Session FA3, the discussion focuses on the following topics: The Effect of Long Duration Space Flight on the Acquisition of Predictable Targets in Three Dimensional Space; Effects of Microgravity on Spinal Reflex Mechanisms; Three Dimensional Head Movement Control During Locomotion After Long-Duration Space Flight; Human Body Shock Wave Transmission Properties After Long Duration Space Flight; Adaptation of Neuromuscular Activation Patterns During Locomotion After Long Duration Space Flight; Balance Control Deficits Following Long-Duration Space Flight; Influence of Weightlessness on Postural Muscular Activity Coordination; and The Use of Inflight Foot Pressure as a Countermeasure to Neuromuscular Degradation.

14 CFR 460.45 - Operator informing space flight participant of risk.

Code of Federal Regulations, 2014 CFR

2014-01-01

... understanding of the hazards and risks of the mission, and each space flight participant must then provide... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Operator informing space flight participant of risk. 460.45 Section 460.45 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL...

14 CFR 460.45 - Operator informing space flight participant of risk.

Code of Federal Regulations, 2013 CFR

2013-01-01

... understanding of the hazards and risks of the mission, and each space flight participant must then provide... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Operator informing space flight participant of risk. 460.45 Section 460.45 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL...

14 CFR 460.45 - Operator informing space flight participant of risk.

Code of Federal Regulations, 2012 CFR

2012-01-01

... understanding of the hazards and risks of the mission, and each space flight participant must then provide... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Operator informing space flight participant of risk. 460.45 Section 460.45 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL...

14 CFR 431.8 - Human space flight.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Human space flight. 431.8 Section 431.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH AND REENTRY OF A REUSABLE LAUNCH VEHICLE (RLV) General § 431.8 Human space flight...

14 CFR 431.8 - Human space flight.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Human space flight. 431.8 Section 431.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH AND REENTRY OF A REUSABLE LAUNCH VEHICLE (RLV) General § 431.8 Human space flight...

14 CFR 431.8 - Human space flight.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Human space flight. 431.8 Section 431.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH AND REENTRY OF A REUSABLE LAUNCH VEHICLE (RLV) General § 431.8 Human space flight...

14 CFR 431.8 - Human space flight.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Human space flight. 431.8 Section 431.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH AND REENTRY OF A REUSABLE LAUNCH VEHICLE (RLV) General § 431.8 Human space flight...

14 CFR 431.8 - Human space flight.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Human space flight. 431.8 Section 431.8 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH AND REENTRY OF A REUSABLE LAUNCH VEHICLE (RLV) General § 431.8 Human space flight...

Alterations in renal stone risk factors after space flight

NASA Technical Reports Server (NTRS)

Whitson, P. A.; Pietrzyk, R. A.; Pak, C. Y.; Cintron, N. M.

1993-01-01

Exposure to the microgravity environment of space produces a number of physiological changes of metabolic and environmental origin that could increase the potential for renal stone formation. Metabolic, environmental and physicochemical factors that influence renal stone risk potential were examined in 24-hour urine samples from astronauts 10 days before launch and on landing day to provide an immediate postflight assessment of these factors. In addition, comparisons were made between male and female crewmembers, and between crewmembers on missions of less than 6 days and those on 6 to 10-day missions. Results suggest that immediately after space flight the risk of calcium oxalate and uric acid stone formation is increased as a result of metabolic (hypercalciuria, hypocitraturia, pH) and environmental (lower urine volume) derangements, some of which could reflect residual effects of having been exposed to microgravity.

Vacuum deposited optical coatings experiment (AO 138-4)

NASA Technical Reports Server (NTRS)

Charlier, Jean

1991-01-01

The aim of this experiment was to test the optical behavior of 20 components and coatings subjected to space exposure. Most of them are commonly used for their reflective or transmittive properties in spaceborne optics. They consist in several kind of metallic and dielectric mirrors designed for the 0.12 to 10 microns spectrum, UV, and NIR bandpass filters, visible, and IR antireflecting coatings, visible/IR dichroic beam splitters, and visible beam splitter. The coatings were deposited on various substrates such as glasses, germanium, magnesium fluoride, quartz, zinc selenide, and kanigened aluminum. Several coating materials were used such as Al, Ag, Au, MgF2, LaF3, ThF3, ThF4, SiO2, TiO2, ZrO2, Al2O3, MgO, Ge, and ZnSe. Five samples of each component were manufactured. Two flight samples were mounted in such a way that one was directly exposed to space and the other looking backwards. The same arrangement was used for the spare samples stored on ground in a box identical to the flight one and they were kept under vacuum during the LDEF mission. Finally, one set of reference components was stocked in a sealed box under a dry nitrogen atmosphere. By comparing the preflight and postflight optical performances of the five samples of each component, it is possible to detect the degradations due to the space exposure.

Breast Cancer Research at NASA

NASA Technical Reports Server (NTRS)

1998-01-01

Time-lapse exposure depicts Bioreactor rotation. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunourous tissues.

14 CFR 417.219 - Data loss flight time and planned safe flight state analyses.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Data loss flight time and planned safe flight state analyses. 417.219 Section 417.219 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION... flight to a condition where the launch vehicle's hazardous debris impact dispersion extends to any...

14 CFR 417.219 - Data loss flight time and planned safe flight state analyses.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Data loss flight time and planned safe flight state analyses. 417.219 Section 417.219 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION... flight to a condition where the launch vehicle's hazardous debris impact dispersion extends to any...

14 CFR 417.219 - Data loss flight time and planned safe flight state analyses.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Data loss flight time and planned safe flight state analyses. 417.219 Section 417.219 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION... flight to a condition where the launch vehicle's hazardous debris impact dispersion extends to any...

14 CFR 417.219 - Data loss flight time and planned safe flight state analyses.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Data loss flight time and planned safe flight state analyses. 417.219 Section 417.219 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION... flight to a condition where the launch vehicle's hazardous debris impact dispersion extends to any...

14 CFR 417.219 - Data loss flight time and planned safe flight state analyses.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Data loss flight time and planned safe flight state analyses. 417.219 Section 417.219 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION... flight to a condition where the launch vehicle's hazardous debris impact dispersion extends to any...

LIFE experiment: isolation of cryptoendolithic organisms from Antarctic colonized sandstone exposed to space and simulated Mars conditions on the international space station.

PubMed

Scalzi, Giuliano; Selbmann, Laura; Zucconi, Laura; Rabbow, Elke; Horneck, Gerda; Albertano, Patrizia; Onofri, Silvano

2012-06-01

Desiccated Antarctic rocks colonized by cryptoendolithic communities were exposed on the International Space Station (ISS) to space and simulated Mars conditions (LiFE-Lichens and Fungi Experiment). After 1.5 years in space samples were retrieved, rehydrated and spread on different culture media. Colonies of a green alga and a pink-coloured fungus developed on Malt-Agar medium; they were isolated from a sample exposed to simulated Mars conditions beneath a 0.1 % T Suprasil neutral density filter and from a sample exposed to space vacuum without solar radiation exposure, respectively. None of the other flight samples showed any growth after incubation. The two organisms able to grow were identified at genus level by Small SubUnit (SSU) and Internal Transcribed Spacer (ITS) rDNA sequencing as Stichococcus sp. (green alga) and Acarospora sp. (lichenized fungal genus) respectively. The data in the present study provide experimental information on the possibility of eukaryotic life transfer from one planet to another by means of rocks and of survival in Mars environment.

LIFE Experiment: Isolation of Cryptoendolithic Organisms from Antarctic Colonized Sandstone Exposed to Space and Simulated Mars Conditions on the International Space Station

NASA Astrophysics Data System (ADS)

Scalzi, Giuliano; Selbmann, Laura; Zucconi, Laura; Rabbow, Elke; Horneck, Gerda; Albertano, Patrizia; Onofri, Silvano

2012-06-01

Desiccated Antarctic rocks colonized by cryptoendolithic communities were exposed on the International Space Station (ISS) to space and simulated Mars conditions (LiFE— Lichens and Fungi Experiment). After 1.5 years in space samples were retrieved, rehydrated and spread on different culture media. Colonies of a green alga and a pink-coloured fungus developed on Malt-Agar medium; they were isolated from a sample exposed to simulated Mars conditions beneath a 0.1 % T Suprasil neutral density filter and from a sample exposed to space vacuum without solar radiation exposure, respectively. None of the other flight samples showed any growth after incubation. The two organisms able to grow were identified at genus level by Small SubUnit (SSU) and Internal Transcribed Spacer (ITS) rDNA sequencing as Stichococcus sp. (green alga) and Acarospora sp. (lichenized fungal genus) respectively. The data in the present study provide experimental information on the possibility of eukaryotic life transfer from one planet to another by means of rocks and of survival in Mars environment.

Characterization of material surfaces exposed to atomic oxygen on space shuttle missions

NASA Technical Reports Server (NTRS)

Fromhold, A. T.

1985-01-01

Material samples prepared for exposure to ambient atomic oxygen encountered during space shuttle flights in low Earth orbit were characterized by the experimental techniques of ELLIPSOMETRY, ESCA, PIXE, and RBS. The first group of samples, which were exposed during the STS-8 mission, exhibited some very interesting results. The second group of samples, which are to be exposed during the upcoming STS-17 mission, have been especially prepared to yield quantitative information on the optical changes, oxygen solution, and surface layer formation on metal films of silver, gold, nickel, chromium, aluminum, platinum, and palladium evaporated onto optically polished silicon wafers.

Status of NASA In-Space Propulsion Technologies and Their Infusion Potential

NASA Technical Reports Server (NTRS)

Anderson, David; Pencil, Eric; Vento, Dan; Peterson, Todd; Dankanich, John; Hahne, David; Munk, Michelle

2011-01-01

Since 2001, the In-Space Propulsion Technology (ISPT) program has been developing in-space propulsion technologies that will enable or enhance NASA robotic science missions. These in-space propulsion technologies have broad applicability to future competed Discovery and New Frontiers mission solicitations, and are potentially enabling for future NASA flagship and sample return missions currently being considered. This paper provides status of the technology development of several in-space propulsion technologies that are ready for infusion into future missions. The technologies that are ready for flight infusion are: 1) the high-temperature Advanced Material Bipropellant Rocket (AMBR) engine providing higher performance; 2) NASA s Evolutionary Xenon Thruster (NEXT) ion propulsion system, a 0.6-7 kW throttle-able gridded ion system; and 3) Aerocapture technology development with investments in a family of thermal protection system (TPS) materials and structures; guidance, navigation, and control (GN&C) models of blunt-body rigid aeroshells; and aerothermal effect models. Two component technologies that will be ready for flight infusion in FY12/13 are 1) Advanced Xenon Flow Control System, and 2) ultra-lightweight propellant tank technology advancements and their infusion potential will be also discussed. The paper will also describe the ISPT project s future focus on propulsion for sample return missions: 1) Mars Ascent Vehicles (MAV); 2) multi-mission technologies for Earth Entry Vehicles (MMEEV) needed for sample return missions from many different destinations; and 3) electric propulsion for sample return and low cost missions. These technologies are more vehicle-focused, and present a different set of technology infusion challenges. Systems/Mission Analysis focused on developing tools and assessing the application of propulsion technologies to a wide variety of mission concepts.

Water and Energy Dietary Requirements and Endocrinology of Human Space Flight

NASA Technical Reports Server (NTRS)

Lane, Helen W.; Feeback, Daniel L.

2002-01-01

Fluid and energy metabolism and related endocrine changes have been studied nearly from the beginning of human space flight in association with short- and long-duration flights. Fluid and electrolyte nutrition status is affected by many factors including the microgravity environment, stress, changes in body composition, diet, exercise habits, sleep cycles, and ambient temperature and humidity conditions. Space flight exposes astronauts to all these factors and consequently poses significant challenges to establishing dietary water, sodium, potassium, and energy recommendations. The purpose of this article is to review the results of ground-based and space flight research studies that have led to current water, electrolyte, and energy dietary requirements for humans during space flight and to give an overview of related endocrinologic changes that have been observed in humans during short- and long-duration space flight.

Effects of Short- and Long-Duration Space Flight on Neuromuscular Function

NASA Technical Reports Server (NTRS)

Buxton, Roxanne E.; Spiering, Barry A.; Ryder, Jeffrey W.; Ploutz-Snyder, Lori L.; Bloomberg, Jacob J.

2010-01-01

The Functional Task Tests (FTT) is an interdisciplinary study designed to correlate the changes in functional tasks (such as emergency egress, ladder climbing, and hatch opening) with changes in neuromuscular, cardiovascular, and sensorimotor function. One aspect of the FTT, the neuromuscular function test, is used to investigate the neuromuscular component underlying changes in the ability of astronauts to perform functional tasks (representative of critical mission tasks) safely and quickly after flight. PURPOSE: To describe neuromuscular function after short- and long-duration space flight. METHODS: To date, 5 crewmembers on short-duration (10- to 15-day) missions and 3 on long-duration missions have participated. Crewmembers were assessed 30 days before flight, on landing day (short-duration subjects only) and 1, 6, and 30 days after landing. The interpolated twitch technique, which utilizes a combination of maximal voluntary contractions and electrically evoked contractions, was used to assess the maximal voluntary isometric force (MIF) and central activation capacity of the knee extensors. Leg-press and bench-press devices were used to assess MIF and maximal dynamic power of the lower and upper body respectively. Specifically, power was measured during concentric-only ballistic throws of the leg-press sled and bench-press bar loaded to 40% and 30% of MIF respectively. RESULTS: Data are currently being collected from both Shuttle and ISS crewmembers. Emerging data indicate that measures of knee extensor muscle function are decreased with long-duration flight. DISCUSSION: The relationships between flight duration, neural drive, and muscle performance are of particular interest. Ongoing research will add to the current sample size and will focus on defining changes in muscle performance measures after long-duration space flight.

Space Flight Software Development Software for Intelligent System Health Management

NASA Technical Reports Server (NTRS)

Trevino, Luis C.; Crumbley, Tim

2004-01-01

The slide presentation examines the Marshall Space Flight Center Flight Software Branch, including software development projects, mission critical space flight software development, software technical insight, advanced software development technologies, and continuous improvement in the software development processes and methods.

DomeGene Sample Removal from Cell Biology Experiment Facility (CBEF)

NASA Image and Video Library

2009-03-28

ISS018-E-044268 (28 March 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 18/19 flight engineer, works on an experiment at the Saibo biological experiment rack in the Kibo laboratory of the International Space Station.

Vascular Blood Collection stowage in MELFI

NASA Image and Video Library

2010-08-26

ISS024-E-012555 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, replaces a dewar tray containing biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Vascular Blood Collection stowage in MELFI

NASA Image and Video Library

2010-08-26

ISS024-E-012553 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, replaces a dewar tray containing biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

14 CFR § 1214.101 - Eligibility for flight of a non-U.S. government reimbursable payload on the Space Shuttle.

Code of Federal Regulations, 2014 CFR

2014-01-01

.... government reimbursable payload on the Space Shuttle. § 1214.101 Section § 1214.101 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle... non-U.S. government reimbursable payload on the Space Shuttle. To be eligible for flight on the Space...

Effects of long-term space condition on cell ultrastructure and the molecular level change of the tomato

NASA Astrophysics Data System (ADS)

Jinying, L.; Min, L.; Huai, X.; Yi, P.; Chunhua, Z.; Nechitalo, G.

Effects of long-term exposure to physical factors of space flight on dormant seeds were studied on plants derived from tomato seeds flown for 6 years on board of the space station MIR Upon return to the Earth the seeds were germinated and grown to maturity Samples of plants were compared to plants from parallel ground-based controls Various differences of ultrastructure of the tomato leaf cell were observed with an electron microscope One plant carried by space station has the anatomy of leaves with a three-layered palisade tissue and other plants similar with ground controls have the anatomy of leaves with a one-layered palisade tissue The number of starch grains per chloroplast of every space-treated tomato leaf increased significantly compared with that of the ground control The leaf cell walls of two plants carried by space station became contracted and deformed The size of chloroplast in some space-treated plants was larger and the lamellae s structure of some chloroplasts turned curvature and loose The results obtained point out to significant changes occurring on the molecular level among the space-flight treated seedlings and the ground control The leaves of plants were used for AFLP Amplification Fragment Length Polymorphism analysis For the first generation space-flight treated tomato plants among 64 pairs of primers used in this experiment 43 primers generated the same DNA bands type and 21 primers generated a different DNA band type 2582 DNA bands were produced among which 34 DNA bands were polymorphic with the percentage

STS-103 Crew at Breakfast, Suiting, Departing O&C

NASA Technical Reports Server (NTRS)

1999-01-01

The Hubble Space Telescope (HST) team is preparing for NASA's third scheduled service call to Hubble. This mission, STS-103, will launch from Kennedy Space Center aboard the Space Shuttle Discovery. The seven flight crew members for STS-103 are: Commander Curtis L. Brown (his sixth flight), Pilot Scott J. Kelly and European Space Agency (ESA) astronaut Jean-Francois Clervoy (his third flight) will join space walkers Steven L. Smith (his third flight), C. Michael Foale (his fifth flight), John M. Grunsfeld (his third flight) and ESA astronaut Claude Nicollier (his fourth flight). This current video presents a live footage of the seven STS-103 crewmembers eating breakfast, suiting, and departing the O&C (Operations and Checkout) before the 6:50 p.m. lift-off.

Enterprise - Free Flight after Separation from 747

NASA Technical Reports Server (NTRS)

1977-01-01

The Space Shuttle prototype Enterprise flies free of NASA's 747 Shuttle Carrier Aircraft (SCA) during one of five free flights carried out at the Dryden Flight Research Facility, Edwards, California in 1977 as part of the Shuttle program's Approach and Landing Tests (ALT). The tests were conducted to verify orbiter aerodynamics and handling characteristics in preparation for orbital flights with the Space Shuttle Columbia. A tail cone over the main engine area of Enterprise smoothed out turbulent airflow during flight. It was removed on the two last free flights to accurately check approach and landing characteristics. The Space Shuttle Approach and Landings Tests (ALT) program allowed pilots and engineers to learn how the Space Shuttle and the modified Boeing 747 Shuttle Carrier Aircraft (SCA) handled during low-speed flight and landing. The Enterprise, a prototype of the Space Shuttles, and the SCA were flown to conduct the approach and landing tests at the NASA Dryden Flight Research Center, Edwards, California, from February to October 1977. The first flight of the program consisted of the Space Shuttle Enterprise attached to the Shuttle Carrier Aircraft. These flights were to determine how well the two vehicles flew together. Five 'captive-inactive' flights were flown during this first phase in which there was no crew in the Enterprise. The next series of captive flights was flown with a flight crew of two on board the prototype Space Shuttle. Only three such flights proved necessary. This led to the free-flight test series. The free-flight phase of the ALT program allowed pilots and engineers to learn how the Space Shuttle handled in low-speed flight and landing attitudes. For these landings, the Enterprise was flown by a crew of two after it was released from the top of the SCA. The vehicle was released at altitudes ranging from 19,000 to 26,000 feet. The Enterprise had no propulsion system, but its first four glides to the Rogers Dry Lake runway provided realistic, in-flight simulations of how subsequent Space Shuttles would be flown at the end of an orbital mission. The fifth approach and landing test, with the Enterprise landing on the Edwards Air Force Base concrete runway, revealed a problem with the Space Shuttle flight control system that made it susceptible to Pilot-Induced Oscillation (PIO), a potentially dangerous control problem during a landing. Further research using other NASA aircraft, especially the F-8 Digital-Fly-By-Wire aircraft, led to correction of the PIO problem before the first orbital flight. The Enterprise's last free-flight was October 26, 1977, after which it was ferried to other NASA centers for ground-based flight simulations that tested Space Shuttle systems and structure.

Enterprise - Free Flight after Separation from 747

NASA Technical Reports Server (NTRS)

1977-01-01

The Space Shuttle prototype Enterprise flies free after being released from NASA's 747 Shuttle Carrier Aircraft (SCA) during one of five free flights carried out at the Dryden Flight Research Center, Edwards, California in 1977, as part of the Shuttle program's Approach and Landing Tests (ALT). The tests were conducted to verify orbiter aerodynamics and handling characteristics in preparation for orbital flights with the Space Shuttle Columbia. A tail cone over the main engine area of Enterprise smoothed out turbulent airflow during flight. It was removed on the two last free flights to accurately check approach and landing characteristics. The Space Shuttle Approach and Landings Tests (ALT) program allowed pilots and engineers to learn how the Space Shuttle and the modified Boeing 747 Shuttle Carrier Aircraft (SCA) handled during low-speed flight and landing. The Enterprise, a prototype of the Space Shuttles, and the SCA were flown to conduct the approach and landing tests at the NASA Dryden Flight Research Center, Edwards, California, from February to October 1977. The first flight of the program consisted of the Space Shuttle Enterprise attached to the Shuttle Carrier Aircraft. These flights were to determine how well the two vehicles flew together. Five 'captive-inactive' flights were flown during this first phase in which there was no crew in the Enterprise. The next series of captive flights was flown with a flight crew of two on board the prototype Space Shuttle. Only three such flights proved necessary. This led to the free-flight test series. The free-flight phase of the ALT program allowed pilots and engineers to learn how the Space Shuttle handled in low-speed flight and landing attitudes. For these landings, the Enterprise was flown by a crew of two after it was released from the top of the SCA. The vehicle was released at altitudes ranging from 19,000 to 26,000 feet. The Enterprise had no propulsion system, but its first four glides to the Rogers Dry Lake runway provided realistic, in-flight simulations of how subsequent Space Shuttles would be flown at the end of an orbital mission. The fifth approach and landing test, with the Enterprise landing on the Edwards Air Force Base concrete runway, revealed a problem with the Space Shuttle flight control system that made it susceptible to Pilot-Induced Oscillation (PIO), a potentially dangerous control problem during a landing. Further research using other NASA aircraft, especially the F-8 Digital-Fly-By-Wire aircraft, led to correction of the PIO problem before the first orbital flight. The Enterprise's last free-flight was October 26, 1977, after which it was ferried to other NASA centers for ground-based flight simulations that tested Space Shuttle systems and structure.

Intersatellite communications optoelectronics research at the Goddard Space Flight Center

NASA Technical Reports Server (NTRS)

Krainak, Michael A.

1992-01-01

A review is presented of current optoelectronics research and development at the NASA Goddard Space Flight Center for high-power, high-bandwidth laser transmitters; high-bandwidth, high-sensitivity optical receivers; pointing, acquisition, and tracking components; and experimental and theoretical system modeling at the NASA Goddard Space Flight Center. Program hardware and space flight opportunities are presented.

Estimating the Effects of Astronaut Career Ionizing Radiation Dose Limits on Manned Interplanetary Flight Programs

NASA Technical Reports Server (NTRS)

Koontz, Steven L.; Rojdev, Kristina; Valle, Gerard D.; Zipay, John J.; Atwell, William S.

2013-01-01

Space radiation effects mitigation has been identified as one of the highest priority technology development areas for human space flight in the NASA Strategic Space Technology Investment Plan (Dec. 2012). In this paper we review the special features of space radiation that lead to severe constraints on long-term (more than 180 days) human flight operations outside Earth's magnetosphere. We then quantify the impacts of human space radiation dose limits on spacecraft engineering design and development, flight program architecture, as well as flight program schedule and cost. A new Deep Space Habitat (DSH) concept, the hybrid inflatable habitat, is presented and shown to enable a flexible, affordable approach to long term manned interplanetary flight today.

NASA Tests 2nd RS-25 Flight Engine for Space Launch System

NASA Image and Video Library

2018-01-16

On Jan. 16, 2018, engineers at NASA’s Stennis Space Center in Mississippi conducted a certification test of another RS-25 engine flight controller on the A-1 Test Stand at Stennis Space Center. The 365-second, full-duration test came a month after the space agency capped a year of RS-25 testing with a flight controller test in mid-December. For the “green run” test the flight controller was installed on RS-25 developmental engine E0528 and fired just as during an actual launch. Once certified, the flight controller will be removed and installed on a flight engine for use by NASA’s new deep-space rocket, the Space Launch System (SLS).

View of human problems to be addressed for long-duration space flights

NASA Technical Reports Server (NTRS)

Berry, C. A.

1973-01-01

Review of the principal physiological changes seen in space flight, and discussion of various countermeasures which may prove to be useful in combating these changes in long-term space flight. A number of transient changes seen in Apollo astronauts following space flights are discussed, including cardiovascular and hemodynamic responses to weightlessness, musculoskeletal changes, changes in fluid and electrolyte balance, microbiological changes, and vestibular effects. A number of countermeasures to the effects of space flight on man are cited, including exercise, medication, diet, lower-body negative pressure, gradient positive pressure, venous occlusion cuffs, and others. A detailed review is then made of a number of psychological factors bearing on the ability of the human organism to withstand the rigors of long space flights.

Space Shuttle Projects Overview to Columbia Air Forces War College

NASA Technical Reports Server (NTRS)

Singer, Jody; McCool, Alex (Technical Monitor)

2000-01-01

This paper presents, in viewgraph form, a general overview of space shuttle projects. Some of the topics include: 1) Space Shuttle Projects; 2) Marshall Space Flight Center Space Shuttle Projects Office; 3) Space Shuttle Propulsion systems; 4) Space Shuttle Program Major Sites; 5) NASA Office of Space flight (OSF) Center Roles in Space Shuttle Program; 6) Space Shuttle Hardware Flow; and 7) Shuttle Flights To Date.

Legacy of Biomedical Research During the Space Shuttle Program

NASA Technical Reports Server (NTRS)

Hayes, Judith C.

2011-01-01

The Space Shuttle Program provided many opportunities to study the role of spaceflight on human life for over 30 years and represented the longest and largest US human spaceflight program. Outcomes of the research were understanding the effect of spaceflight on human physiology and performance, countermeasures, operational protocols, and hardware. The Shuttle flights were relatively short, < 16 days and routinely had 4 to 6 crewmembers for a total of 135 flights. Biomedical research was conducted on the Space Shuttle using various vehicle resources. Specially constructed pressurized laboratories called Spacelab and SPACEHAB housed many laboratory instruments to accomplish experiments in the Shuttle s large payload bay. In addition to these laboratory flights, nearly every mission had dedicated human life science research experiments conducted in the Shuttle middeck. Most Shuttle astronauts participated in some life sciences research experiments either as test subjects or test operators. While middeck experiments resulted in a low sample per mission compared to many Earth-based studies, this participation allowed investigators to have repetition of tests over the years on successive Shuttle flights. In addition, as a prelude to the International Space Station (ISS), NASA used the Space Shuttle as a platform for assessing future ISS hardware systems and procedures. The purpose of this panel is to provide an understanding of science integration activities required to implement Shuttle research, review biomedical research, characterize countermeasures developed for Shuttle and ISS as well as discuss lessons learned that may support commercial crew endeavors. Panel topics include research integration, cardiovascular physiology, neurosciences, skeletal muscle, and exercise physiology. Learning Objective: The panel provides an overview from the Space Shuttle Program regarding research integration, scientific results, lessons learned from biomedical research and countermeasure development.

Advanced biosensors for monitoring astronauts' health during long-duration space missions.

PubMed

Roda, Aldo; Mirasoli, Mara; Guardigli, Massimo; Zangheri, Martina; Caliceti, Cristiana; Calabria, Donato; Simoni, Patrizia

2018-07-15

Long-duration space missions pose important health concerns for astronauts, especially regarding the adverse effects of microgravity and exposure to high-energy cosmic rays. The long-term maintenance of crew health and performance mainly relies on prevention, early diagnoses, condition management, and medical interventions in situ. In-flight biosensor diagnostic devices and medical procedures must use few resources and operate in a microgravity environment, which complicates the collection and management of biological samples. Moreover, the biosensors must be certified for in-flight operation according to strict design and safety regulations. Herein, we report on the state of the art and recent advances in biosensing diagnostic instrumentation for monitoring astronauts' health during long-duration space missions, including portable and wearable biosensors. We discuss perspectives on new-format biosensors in autonomous space clinics. We also describe our own work in developing biosensing devices for non-invasively diagnosing space-related diseases, and how they are used in long-duration missions. Finally, we discuss the benefits of space exploration for Earth-based medicine. Copyright © 2018 Elsevier B.V. All rights reserved.

Movable Ground Based Recovery System for Reuseable Space Flight Hardware

NASA Technical Reports Server (NTRS)

Sarver, George L. (Inventor)

2013-01-01

A reusable space flight launch system is configured to eliminate complex descent and landing systems from the space flight hardware and move them to maneuverable ground based systems. Precision landing of the reusable space flight hardware is enabled using a simple, light weight aerodynamic device on board the flight hardware such as a parachute, and one or more translating ground based vehicles such as a hovercraft that include active speed, orientation and directional control. The ground based vehicle maneuvers itself into position beneath the descending flight hardware, matching its speed and direction and captures the flight hardware. The ground based vehicle will contain propulsion, command and GN&C functionality as well as space flight hardware landing cushioning and retaining hardware. The ground based vehicle propulsion system enables longitudinal and transverse maneuverability independent of its physical heading.

Animal Enclosure Module (AEM)

NASA Technical Reports Server (NTRS)

1998-01-01

The primary objective of this research project is to test the hypothesis that corticosteroids contribute to the adverse skeletal effects of space flight. To achieve this objective, serum corticosteroids, which are known to increase during space flight, must be maintained at normal physiologic levels in flight rats by a combination of adrenalectomy and corticosteroid supplementation via implanted hormone pellets. Bone analyses in these animals will then be compared to those of intact flight rats that, based on past experience, will undergo corticosteroid excess and bone loss during space flight. The results will reveal whether maintaining serum corticosteroids at physiologic levels in flight rats affects the skeletal abnormalities that normally develop during space flight. A positive response to this question would indicate that the bone loss and decreased bone formation associated with space flight are mediated, at least in part, by corticosteroid excess.

Microgravity

NASA Image and Video Library

1997-03-11

This photo shows the access through the internal airlock (bottom right) on the Microgravity Science Glovebox (MSG) being developed by the European Space Agency (ESA) and NASA for use aboard the International Space Station (ISS). The airlock will allow the insertion or removal of equipment and samples without opening the working volume of the glovebox. Scientists will use the MSG to carry out multidisciplinary studies in combustion science, fluid physics and materials science. The MSG is managed by NASA's Marshall Space Flight Center (MSFC). Photo Credit: NASA/MSFC

Polymers Erosion and Contamination Experiment Being Developed

NASA Technical Reports Server (NTRS)

deGroh, Kim K.; Banks, Bruce A.; Barney-Barton, Elyse A.; Sechkar, Edward; Hunt, Patricia

1999-01-01

The Polymers Erosion and Contamination Experiment (PEACE) is currently being developed at the NASA Lewis Research Center by the Electro-Physics Branch in conjunction with students and faculty from Hathaway Brown School in Cleveland. The experiment is a Get Away Special Canister shuttle flight experiment sponsored by the American Chemical Society. The two goals of this experiment are (1) to measure ram atomic oxygen erosion rates of approximately 40 polymers that have potential use in space applications and (2) to validate a method for identifying sources of silicone contamination that occur in the shuttle bay. Equipment to be used in this flight experiment is shown in the schematic diagram. Spacecraft materials subjected to attack by atomic oxygen in the space environment experience significant degradation over the span of a typical mission. Therefore, learning the rates of atomic oxygen erosion of a wide variety of polymers would be of great benefit to future missions. PEACE will use two independent techniques to determine the atomic oxygen erosion rates of polymers. Large (1-in.-diameter) samples will be used for obtaining mass loss. Preflight and postflight dehydrated masses will be obtained, and the mass lost during flight will be determined. Small (0.5-in.-diameter) samples will be protected with isolated particles (such as NaCl crystals) and then exposed to the space environment. After flight, the protective particles will be removed (washed off) and atomic force microscopy (AFM) will be used to measure the erosion depth from protected mesas. Erosion depth measurements are more sensitive than traditional mass measurements and are very useful for materials with low erosion yields or with very low fluence missions.

Mars Rock Analysis Briefing

NASA Image and Video Library

2013-03-12

Paul Mahaffy, principal investigator for Curiosity's Sample Analysis at Mars (SAM) investigation at NASA's Goddard Space Flight Center in Maryland, answer's a reporters question at a news conference, Tuesday, March 12, 2013 at NASA Headquarters in Washington. The news conference covered the findings that the analysis of the rock sample collected shows ancient Mars could have supported living microbes. Photo Credit: (NASA/Carla Cioffi)

Expedition Six Flight Engineer Pettit uses a chemical/microbial analysis bag to collect water sample

NASA Image and Video Library

2002-12-18

ISS006-E-08628 (18 December 2002) --- Astronaut Donald R. Pettit, Expedition Six NASA ISS science officer, is pictured in the Zvezda Service Module on the International Space Station (ISS) during the scheduled Week 3 potable water sampling and on-orbit chemical/microbial analysis of the SM environment control and life support system.

Expedition Six Flight Engineer Pettit uses a chemical/microbial analysis bag to collect water sample

NASA Image and Video Library

2002-12-18

ISS006-E-08616 (18 December 2002) --- Astronaut Donald R. Pettit, Expedition Six NASA ISS science officer, is pictured in the Zvezda Service Module on the International Space Station (ISS) during the scheduled Week 3 potable water sampling and on-orbit chemical/microbial analysis of the SM environment control and life support system.

STS-125 Flight Controllers on Console During HST Grapple - Orbit 1. Flight Director: Tony Ceccacci

NASA Image and Video Library

2009-05-13

JSC2009-E-119745 (13 May 2009) --- Flight director Tony Ceccacci (left) and astronaut Dan Burbank, STS-125 spacecraft communicator (CAPCOM), monitor data at their consoles in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during flight day three activities. The Hubble Space Telescope, grappled by Space Shuttle Atlantis? remote manipulator system (RMS), is visible on one of the big screens.

STS-125 Flight Controllers on Console During HST Grapple - Orbit 1. Flight Director: Tony Ceccacci

NASA Image and Video Library

2009-05-13

JSC2009-E-119746 (13 May 2009) --- Flight director Tony Ceccacci (left) and astronaut Dan Burbank, STS-125 spacecraft communicator (CAPCOM), monitor data at their consoles in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during flight day three activities. The Hubble Space Telescope, grappled by Space Shuttle Atlantis? remote manipulator system (RMS), is visible on one of the big screens.

Enterprise - Free Flight after Separation from 747

NASA Technical Reports Server (NTRS)

1977-01-01

The Space Shuttle prototype Enterprise flies free after being released from NASA's 747 Shuttle Carrier Aircraft (SCA) over Rogers Dry Lake during the second of five free flights carried out at the Dryden Flight Research Center, Edwards, California, as part of the Shuttle program's Approach and Landing Tests (ALT) in 1977. The tests were conducted to verify orbiter aerodynamics and handling characteristics in preparation for orbital flights with the Space Shuttle Columbia. A tail cone over the main engine area of Enterprise smoothed out turbulent airflow during flight. It was removed on the two last free flights to accurately check approach and landing characteristics. A series of test flights during which Enterprise was taken aloft atop the SCA, but was not released, preceded the free flight tests. The Space Shuttle Approach and Landings Tests (ALT) program allowed pilots and engineers to learn how the Space Shuttle and the modified Boeing 747 Shuttle Carrier Aircraft (SCA) handled during low-speed flight and landing. The Enterprise, a prototype of the Space Shuttles, and the SCA were flown to conduct the approach and landing tests at the NASA Dryden Flight Research Center, Edwards, California, from February to October 1977. The first flight of the program consisted of the Space Shuttle Enterprise attached to the Shuttle Carrier Aircraft. These flights were to determine how well the two vehicles flew together. Five 'captive-inactive' flights were flown during this first phase in which there was no crew in the Enterprise. The next series of captive flights was flown with a flight crew of two on board the prototype Space Shuttle. Only three such flights proved necessary. This led to the free-flight test series. The free-flight phase of the ALT program allowed pilots and engineers to learn how the Space Shuttle handled in low-speed flight and landing attitudes. For these landings, the Enterprise was flown by a crew of two after it was released from the top of the SCA. The vehicle was released at altitudes ranging from 19,000 to 26,000 feet. The Enterprise had no propulsion system, but its first four glides to the Rogers Dry Lake runway provided realistic, in-flight simulations of how subsequent Space Shuttles would be flown at the end of an orbital mission. The fifth approach and landing test, with the Enterprise landing on the Edwards Air Force Base concrete runway, revealed a problem with the Space Shuttle flight control system that made it susceptible to Pilot-Induced Oscillation (PIO), a potentially dangerous control problem during a landing. Further research using other NASA aircraft, especially the F-8 Digital-Fly-By-Wire aircraft, led to correction of the PIO problem before the first orbital flight. The Enterprise's last free-flight was October 26, 1977, after which it was ferried to other NASA centers for ground-based flight simulations that tested Space Shuttle systems and structure.

14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Time...

14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Time...

14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Time...

14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Time...

14 CFR 121.493 - Flight time limitations: Flight engineers and flight navigators.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Flight time limitations: Flight engineers and flight navigators. 121.493 Section 121.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Time...

Acquisition of a Biomedical Database of Acute Responses to Space Flight during Commercial Personal Suborbital Flights

NASA Technical Reports Server (NTRS)

Charles, John B.; Richard, Elizabeth E.

2010-01-01

There is currently too little reproducible data for a scientifically valid understanding of the initial responses of a diverse human population to weightlessness and other space flight factors. Astronauts on orbital space flights to date have been extremely healthy and fit, unlike the general human population. Data collection opportunities during the earliest phases of space flights to date, when the most dynamic responses may occur in response to abrupt transitions in acceleration loads, have been limited by operational restrictions on our ability to encumber the astronauts with even minimal monitoring instrumentation. The era of commercial personal suborbital space flights promises the availability of a large (perhaps hundreds per year), diverse population of potential participants with a vested interest in their own responses to space flight factors, and a number of flight providers interested in documenting and demonstrating the attractiveness and safety of the experience they are offering. Voluntary participation by even a fraction of the flying population in a uniform set of unobtrusive biomedical data collections would provide a database enabling statistical analyses of a variety of acute responses to a standardized space flight environment. This will benefit both the space life sciences discipline and the general state of human knowledge.

NAAMES Photo Essay

NASA Image and Video Library

2017-12-08

A low ceiling of broken clouds offers opportunities for researches to sample clouds during part of the flight and clear air during other parts of the flight. --- The North Atlantic Aerosols and Marine Ecosystems Study (NAAMES) is a five year investigation to resolve key processes controlling ocean system function, their influences on atmospheric aerosols and clouds and their implications for climate. Michael Starobin joined the NAAMES field campaign on behalf of Earth Expeditions and NASA Goddard Space Flight Center’s Office of Communications. He presented stories about the important, multi-disciplinary research being conducted by the NAAMES team, with an eye towards future missions on the NASA drawing board. This is a NAAMES photo essay put together by Starobin, a collection of 49 photographs and captions. Photo and Caption Credit: Michael Starobin NASA image use policy NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Microbial Surveillance of Potable Water Sources of the International Space Station

NASA Technical Reports Server (NTRS)

Bruce, Rebekah J.; Ott, C. Mark; Skuratov, Vladimir M.; Pierson, Duane L.

2005-01-01

To mitigate risk to the crew, the microbial surveillance of the quality of potable water sources of the International Space Station (ISS) has been ongoing since before the arrival of the first permanent crew. These water sources have included stored ground-supplied water, water produced by the shuttle fuel cells during flight, and ISS humidity condensate that is reclaimed and processed. Monitoring was accomplished using a self-contained filter designed to allow bacterial growth and enumeration during flight. Upon return to earth, microbial isolates were identified using 16S ribosomal gene sequencing. While the predominant isolates were common Gramnegative bacteria including Ralstonia eutropha, Methylobacterium fujisawaense, and Spingomonas paucimobilis, opportunistic pathogens such as Stenotrophomonas maltophilia and Pseudomonas aeruginosa were also isolated. Results of in-flight enumeration have indicated a fluctuation of bacterial counts above system design specifications. Additional in-flight monitoring capability for the specific detection of coliforms was added in 2004; no coliforms have been detected from any potable water source. Neither the bacterial concentrations nor the identification of the isolates recovered from these samples has suggested a threat to crew health.

Long-Duration Space Flight Provokes Pathologic Q-Tc Interval Prolongation

NASA Technical Reports Server (NTRS)

D'Aunno, DOminick S.; Dougherty, Anne H.; DeBlock, Heidi F.; Meck, Janice V.

2002-01-01

Space flight has a profound influence on the cardiovascular and autonomic nervous systems. Alterations in baroreflex function, plasma catecholamine concentrations, and arterial pressure regulation have been observed. Changes in autonomic regulation of cardiac function may lead to serious rhythm disturbances. In fact, ventricular tachycardia has been reported during long-duration space flight. The study aim was to determine the effects of space flight on cardiac conduction. Methods and Results: Electrocardiograms (ECGs) and serum electrolytes were obtained before and after short-duration (SD) (4-16 days) and long-duration (LD) (4-6 months) missions. Holter recordings were obtained from 3 different subjects before, during and after a 4-month mission. P-R, R-R, and Q-T intervals were measured manually in a random, blinded fashion and Bazzet's formula used to correct the Q-T interval (Q-Tc). Space flight had no clinically significant effect on electrolyte concentrations. P-R and RR intervals were decreased after SD flight (p<0.05) and recovered 3 days after landing. In the same subjects, P-R and Q-Tc intervals were prolonged after LD flight (p<0.01). Clinically significant Q-Tc prolongation (>0.44 sec) occurred during the first month of flight and persisted until 3 days after landing (p<0.01). Conclusions - Space flight alters cardiac conduction with more ominous changes seen with LD missions. Alterations in autonomic tone may explain ECG changes associated with space flight. Primary cardiac changes may also contribute to the conduction changes with LD flight. Q-Tc prolongation may predispose astronauts to ventricular arrhythmias during and after long-duration space flight.

Electron Micrographs of Quail Limb Bones formed in microgravity

NASA Technical Reports Server (NTRS)

2003-01-01

Electron micrographs of quail limb bones that formed under the influence of microgravity show decreased mineralization compared to bones formed in normal gravity. The letters B and C indicate bone and cartilage sides of the sample, respectively, with the arrows marking the junction between bone and cartilage cells. The asterisks indicate where mineralization begins. The bone that developed during spaceflight (top) shows less mineral compared to the control sample (bottom); the control sample clearly shows mineral deposits (dark spots) that are absent in the flight sample. Quail eggs are small and develop quickly, making them ideal for space experiments. In late 2001, the Avian Development Facility (ADF) made its first flight and carried eggs used in two investigations, development and function of the irner-ear balance system in normal and altered gravity environments, and skeletal development in embryonic quail.

Effect of space flight on cytokine production

NASA Astrophysics Data System (ADS)

Sonnenfeld, Gerald

Space flight has been shown to alter many immunological responses. Among those affected are the production of cytokines, Cytokines are the messengers of the immune system that facilitate communication among cells that allow the interaction among cells leading to the development of immune responses. Included among the cytokines are the interferons, interleukins, and colony stimulating factors. Cytokines also facilitate communication between the immune system and other body systems, such as the neuroendocrine and musculoskeletal systems. Some cytokines also have direct protective effects on the host, such as interferon, which can inhibit the replication of viruses. Studies in both humans and animals indicate that models of space flight as well as actual space flight alter the production and action of cytokines. Included among these changes are altered interferon production, altered responsiveness of bone marrow cells to granulocyte/monocyte-colony stimulating factor, but no alteration in the production of interleukin-3. This suggests that there are selective effects of space flight on immune responses, i.e. not all cytokines are affected in the same fashion by space flight. Tissue culture studies also suggest that there may be direct effects of space flight on the cells responsible for cytokine production and action. The results of the above study indicate that the effects of space flight on cytokines may be a fundamental mechanism by which space flight not only affects immune responses, but also other biological systems of the human.

The main changes in plant exposured during space flight missions and prospectives of biological studies on ISS

NASA Astrophysics Data System (ADS)

Nechitailo, Galina S.; Kuznetsov, Anatoli

The fundamental result of biological investigations with plants in space flight is an experimen-tal evidence of vegetative growth from seeds to harvest, with passing of all those stages of development when the plant can be used for food. The changes of plant observed after space flight mission gives a knowledge, which has to be used for precise selection of the plants for future space missions. The experimental investigation of the plants under space flight condi-tions showed that the germinations ability, rate of growth and biometric parameters decrease in comparison with Earth plants. The first two of these factors can be caused by the influence of specific cultivation in space, but the third factor is caused by the influence of space flight conditions, in particular, microgravity. The investigations of germination, plants deaths at var-ious stages of growth, survival probability, and recessive mutations indicated an impairment of genetic apparatus of meristem cells, which results the lethal effect at various stages of develop-ment. The density of paramagnetic centers in seeds was measured in order to determine the free radical concentration under space flight conditions. The concentration of paramagnetic centers is higher for plants with high density of these centers initially. Perhaps, the observed genetic effects in plants under space flight conditions are connected with free radicals. The changes are observed in cells of the plants. The changes included twist, contraction and deformation of the cell walls, curvature and loose arrangement of lamellae in chloroplasts, break of outer membrane of mitochondria and disappearance of mitochondria cristae. A large number of stach grains is observed in chloroplasts. The seeds of various plants were successfully used in space flights: welsh onion, wheat, peas, maize, barley, tomatoes, etc. Mostly stabe plants to space flight factors are found as peas, wheat and tomatoes. Ten generation of wheat and tomatoues exposed in space flights were grown on Earth after flight. The investigation of these plants is used for recommendations of next space flight missions on ISS including new sorts of plants.

Apollo experience report. Crew-support activities for experiments performed during manned space flight

NASA Technical Reports Server (NTRS)

Mckee, J. W.

1974-01-01

Experiments are performed during manned space flights in an attempt to acquire knowledge that can advance science and technology or that can be applied to operational techniques for future space flights. A description is given of the procedures that the personnel who are directly assigned to the function of crew support at the NASA Lyndon B. Johnson Space Center use to prepare for and to conduct experiments during space flight.

Research and Technology, 1987, Goddard Space Flight Center

NASA Technical Reports Server (NTRS)

Guerny, Gene (Editor); Moe, Karen (Editor); Paddack, Steven (Editor); Soffen, Gerald (Editor); Sullivan, Walter (Editor); Ballard, Jan (Editor)

1987-01-01

Research at Goddard Space Flight Center during 1987 is summarized. Topics addressed include space and earth sciences, technology, flight projects and mission definition studies, and institutional technology.

Current Characteristics and Trends of the Tracked Satellite Population in the Human Space Flight Regime

NASA Technical Reports Server (NTRS)

Johnson, Nicholas L.

2006-01-01

Since the end of the Apollo program in 1972, human space flight has been restricted to altitudes below 600 km above the Earth s surface with most missions restricted to a ceiling below 400 km. An investigation of the tracked satellite population transiting and influencing the human space flight regime during the past 11 years (equivalent to a full solar cycle) has recently been completed. The overall effects of satellite breakups and solar activity are typically less pronounced in the human space flight regime than other regions of low Earth orbit. As of January 2006 nearly 1500 tracked objects resided in or traversed the human space flight regime, although two-thirds of these objects were in orbits of moderate to high eccentricity, significantly reducing their effect on human space flight safety. During the period investigated, the spatial density of tracked objects in the 350-400 km altitude regime of the International Space Station demonstrated a steady decline, actually decreasing by 50% by the end of the period. On the other hand, the region immediately above 600 km experienced a significant increase in its population density. This regime is important for future risk assessments, since this region represents the reservoir of debris which will influence human space flight safety in the future. The paper seeks to put into sharper perspective the risks posed to human space flight by the tracked satellite population, as well as the influences of solar activity and the effects of compliance with orbital debris mitigation guidelines on human space flight missions. Finally, the methods and successes of characterizing the population of smaller debris at human space flight regimes are addressed.

14 CFR 121.425 - Flight engineers: Initial and transition flight training.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Flight engineers: Initial and transition flight training. 121.425 Section 121.425 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.425 Flight engineers: Initial and transition flight training. (a) Initial and transition flight...

14 CFR 121.425 - Flight engineers: Initial and transition flight training.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Flight engineers: Initial and transition flight training. 121.425 Section 121.425 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.425 Flight engineers: Initial and transition flight training. (a) Initial and transition flight...

14 CFR 121.426 - Flight navigators: Initial and transition flight training.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight navigators: Initial and transition flight training. 121.426 Section 121.426 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.426 Flight navigators: Initial and transition flight training. (a) Initial and transition flight...

14 CFR 121.426 - Flight navigators: Initial and transition flight training.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Flight navigators: Initial and transition flight training. 121.426 Section 121.426 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.426 Flight navigators: Initial and transition flight training. (a) Initial and transition flight...

14 CFR 121.425 - Flight engineers: Initial and transition flight training.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Flight engineers: Initial and transition flight training. 121.425 Section 121.425 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.425 Flight engineers: Initial and transition flight training. (a) Initial and transition flight...

14 CFR 121.426 - Flight navigators: Initial and transition flight training.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Flight navigators: Initial and transition flight training. 121.426 Section 121.426 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.426 Flight navigators: Initial and transition flight training. (a) Initial and transition flight...

14 CFR 121.426 - Flight navigators: Initial and transition flight training.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Flight navigators: Initial and transition flight training. 121.426 Section 121.426 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.426 Flight navigators: Initial and transition flight training. (a) Initial and transition flight...

14 CFR 121.425 - Flight engineers: Initial and transition flight training.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Flight engineers: Initial and transition flight training. 121.425 Section 121.425 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.425 Flight engineers: Initial and transition flight training. (a) Initial and transition flight...

14 CFR 121.425 - Flight engineers: Initial and transition flight training.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight engineers: Initial and transition flight training. 121.425 Section 121.425 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... § 121.425 Flight engineers: Initial and transition flight training. (a) Initial and transition flight...

A Technique for the Assessment of Flight Operability Characteristics of Human Rated Spacecraft

NASA Technical Reports Server (NTRS)

Crocker, Alan

2010-01-01

In support of new human rated spacecraft development programs, the Mission Operations Directorate at NASA Johnson Space Center has implemented a formal method for the assessment of spacecraft operability. This "Spacecraft Flight Operability Assessment Scale" defines six key themes of flight operability, with guiding principles and goals stated for each factor. A standardized rating technique provides feedback that is useful to the operations, design and program management communities. Applicability of this concept across the program structure and life cycle is addressed. Examples of operationally desirable and undesirable spacecraft design characteristics are provided, as is a sample of the assessment scale product.

Esrange Space Center, a Gate to Space

NASA Astrophysics Data System (ADS)

Widell, Ola

Swedish Space Corporation (SSC) is operating the Esrange Space Center in northern Sweden. Space operations have been performed for more than 40 years. We have a unique combination of maintaining balloon and rocket launch operations, and building payloads, providing space vehicles and service systems. Sub-orbital rocket flights with land recovery and short to long duration balloon flights up to weeks are offered. The geographical location, land recovery area and the long term experience makes Swedish Space Corporation and Esrange to an ideal gate for space activities. Stratospheric balloons are primarily used in supporting atmospheric research, validation of satellites and testing of space systems. Balloon operations have been carried out at Esrange since 1974. A large number of balloon flights are yearly launched in cooperation with CNES, France. Since 2005 NASA/CSBF and Esrange provide long duration balloon flights to North America. Flight durations up to 5 days with giant balloons (1.2 Million cubic metres) carrying heavy payload (up to 2500kg) with astronomical instruments has been performed. Balloons are also used as a crane for lifting space vehicles or parachute systems to be dropped and tested from high altitude. Many scientific groups both in US, Europe and Japan have indicated a great need of long duration balloon flights. Esrange will perform a technical polar circum balloon flight during the summer 2008 testing balloon systems and flight technique. We are also working on a permission giving us the opportunity on a circular stratospheric balloon flight around the North Pole.

DomeGene Sample Removal from Cell Biology Experiment Facility (CBEF)

NASA Image and Video Library

2009-03-28

ISS018-E-044235 (28 March 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 18/19 flight engineer, works on an experiment at the Ryutai fluid science experiment rack in the Kibo laboratory of the International Space Station.

Walker photographs BCAT-5 (Binary Colloidal Alloy Test-5) payload

NASA Image and Video Library

2010-10-19

ISS025-E-008239 (19 Oct. 2010) --- NASA astronaut Shannon Walker, Expedition 25 flight engineer, uses a digital still camera to photograph Binary Colloidal Alloy Test-5 (BCAT-5) experiment samples in the Kibo laboratory of the International Space Station.

Vascular Blood Collection stowage in MELFI

NASA Image and Video Library

2010-08-26

ISS024-E-012546 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, prepares to insert biological samples in a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Vascular Blood Collection stowage in MELFI

NASA Image and Video Library

2010-08-26

ISS024-E-012548 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, prepares to insert biological samples in a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

Microgravity

NASA Image and Video Library

2000-06-05

Prof. Kerneth Kelton of Washington University in St. Lous, MO, (L) and Dr. Michael Robinson of NASA's Marshall Space Flight Center (MSFC) examine a titanium-iron silicate (TiFeSiO)sample processed in MSFC's Electrostatic Levitator (ESL) Facility (background). Kelton is investigating undercooling of polytetrahedral phase-forming liquids.

Bowersox works with the WMK in Destiny during Expedition Six

NASA Image and Video Library

2003-02-17

ISS006-E-27226 (17 February 2003) --- Astronaut Kenneth D. Bowersox, Expedition Six mission commander, uses the water microbiology kit (WMK) to collect water samples for in-flight chemistry/microbiology analysis in the Destiny laboratory on the International Space Station (ISS).

FE-2 Stott analyzes Water Samples using the CQQMK in the US Lab

NASA Image and Video Library

2009-10-20

ISS021-E-010311 (20 Oct. 2009) --- NASA astronaut Nicole Stott, Expedition 21 flight engineer, conducts a water quality analysis using the Colorimetric Water Quality Monitoring Kit (CWQMK) in the Destiny laboratory of the International Space Station.

Toxicological Risks During Human Space Exploration

NASA Technical Reports Server (NTRS)

James, John T.; Limero, T. F.; Lam, C. W.; Billica, Roger (Technical Monitor)

2000-01-01

The goal of toxicological risk assessment of human space flight is to identify and quantify significant risks to astronaut health from air pollution inside the vehicle or habitat, and to develop a strategy for control of those risks. The approach to completing a toxicological risk assessment involves data and experience on the frequency and severity of toxicological incidents that have occurred during space flight. Control of these incidents depends on being able to understand their cause from in-flight and ground-based analysis of air samples, crew reports of air quality, and known failures in containment of toxic chemicals. Toxicological risk assessment in exploration missions must be based on an evaluation of the unique toxic hazards presented by the habitat location. For example, lunar and Martian dust must be toxicologically evaluated to determine the appropriate control measures for exploration missions. Experience with near-earth flights has shown that the toxic products from fires present the highest risk to crew health from air pollution. Systems and payload leaks also present a significant hazard. The health risk from toxicity associated with materials offgassing or accumulation of human metabolites is generally well controlled. Early tests of lunar and Martian dust simulants have shown that each posses the potential to cause fibrosis in the lung in a murine model. Toxicological risks from air pollutants in space habitats originate from many sources. A number of risks have been identified through near-earth operations; however, the evaluation of additional new risks present during exploration missions will be a challenge.

Research and technology, 1984: Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

Moorehead, T. W. (Editor)

1984-01-01

The Marshall Space Flight Center conducts research programs in space sciences, materials processing in space, and atmospheric sciences, as well as technology programs in such areas as propulsion, materials, processes, and space power. This Marshall Space Flight Center 1984 Annual Report on Research and Technology contains summaries of the more significant scientific and technical results obtained during FY-84.

Space Flight: The First 30 Years

NASA Technical Reports Server (NTRS)

1991-01-01

A history of space flight from Project Mercury to the Space Shuttle is told from the perspective of NASA flight programs. Details are given on Mercury missions, Gemini missions, Apollo missions, Skylab missions, the Apollo-Soyuz Test Project, and the Space Shuttle missions.

Enterprise Separates from 747 SCA for First Tailcone off Free Flight

NASA Technical Reports Server (NTRS)

1977-01-01

The Space Shuttle prototype Enterprise rises from NASA's 747 Shuttle Carrier Aircraft (SCA) to begin a powerless glide flight back to NASA's Dryden Flight Research Center, Edwards, California, on its fourth of the five free flights in the shuttle program's Approach and Landing Tests (ALT), 12 October 1977. The tests were carried out at Dryden to verify the aerodynamic and control characteristics of the orbiters in preparation for the first space mission with the orbiter Columbia in April 1981. The Space Shuttle Approach and Landings Tests (ALT) program allowed pilots and engineers to learn how the Space Shuttle and the modified Boeing 747 Shuttle Carrier Aircraft (SCA) handled during low-speed flight and landing. The Enterprise, a prototype of the Space Shuttles, and the SCA were flown to conduct the approach and landing tests at the NASA Dryden Flight Research Center, Edwards, California, from February to October 1977. The first flight of the program consisted of the Space Shuttle Enterprise attached to the Shuttle Carrier Aircraft. These flights were to determine how well the two vehicles flew together. Five 'captive-inactive' flights were flown during this first phase in which there was no crew in the Enterprise. The next series of captive flights was flown with a flight crew of two on board the prototype Space Shuttle. Only three such flights proved necessary. This led to the free-flight test series. The free-flight phase of the ALT program allowed pilots and engineers to learn how the Space Shuttle handled in low-speed flight and landing attitudes. For these landings, the Enterprise was flown by a crew of two after it was released from the top of the SCA. The vehicle was released at altitudes ranging from 19,000 to 26,000 feet. The Enterprise had no propulsion system, but its first four glides to the Rogers Dry Lake runway provided realistic, in-flight simulations of how subsequent Space Shuttles would be flown at the end of an orbital mission. The fifth approach and landing test, with the Enterprise landing on the Edwards Air Force Base concrete runway, revealed a problem with the Space Shuttle flight control system that made it susceptible to Pilot-Induced Oscillation (PIO), a potentially dangerous control problem during a landing. Further research using other NASA aircraft, especially the F-8 Digital-Fly-By-Wire aircraft, led to correction of the PIO problem before the first orbital flight. The Enterprise's last free-flight was October 26, 1977, after which it was ferried to other NASA centers for ground-based flight simulations that tested Space Shuttle systems and structure.

Effects of space flight and IGF-1 on immune function

NASA Astrophysics Data System (ADS)

1999-01-01

We tested the hypothesis that insulin-like growth factor-1 (IGF-1) would ameliorate space flight-induced effects on the immune system. Twelve male, Sprague-Dawley rats, surgically implanted with mini osmotic pumps, were subjected to space flight for 10 days on STS-77. Six rats received 10 mg/kg/day of IGF-1 and 6 rats received saline. Flight animals had a lymphocytopenia and granulocytosis which were reversed by IGF-1. Flight animals had significantly higher corticosterone levels than ground controls but IGF-1 did not impact this stress hormone. Therefore, the reversed granulocytosis did not correlate with serum corticosterone. Space flight and IGF-1 also combined to induce a monocytopenia that was not evident in ground control animals treated with IGF-1 or in animals subjected to space flight but given physiological saline. There was a significant increase in spleen weights in vivarium animals treated with IGF-1, however, this change did not occur in flight animals. We observed reduced agonist-induced lymph node cell proliferation by cells from flight animals compared to ground controls. The reduced proliferation was not augmented by IGF-1 treatment. There was enhanced secretion of TNF, IL-6 and NO by flight-animal peritoneal macrophages compared to vivarium controls, however, O2- secretion was not affected. These data suggest that IGF-1 can ameliorate some of the effects of space flight but that space flight can also impact the normal response to IGF-1.

Thermoluminescent dosimetry for LDEF experiment M0006

NASA Technical Reports Server (NTRS)

Chang, J. Y.; Giangano, D.; Kantorcik, T.; Stauber, M.; Snead, L.

1992-01-01

Experiment M0006 on the Long Duration Exposure Facility had as its objective the investigation of space radiation effects on various electronic and optical components, as well as on seed germination. The Grumman Corporate Research Center provided the radiation dosimetric measurements for M0006, comprising the preparation of thermoluminescent dosimeters (TLD) and the subsequent measurement and analysis of flight exposed and control samples. In addition, various laboratory exposures of TLD's with gamma rays and protons were performed to obtain a better understanding of the flight exposures.

Integrated Real Time Contamination Monitor IRTCM

NASA Technical Reports Server (NTRS)

Luttges, W. E.

1976-01-01

Engineering and design work was performed on a monitoring device for particulate and gas contamination to be used in the space shuttle cargo area during launch at altitudes up to 50 km and during return phases of the flight. The gas sampling device consists of ampules filled with specific absorber materials which are opened and/or sealed at preprogrammed intervals. The design eliminates the use of valves which, according to experiments, are never sealing properly at hard vacuum. Methods of analysis including in-flight measuring possibilities are discussed.

Pulse Code Modulation (PCM) encoder handbook for Aydin Vector MMP-900 series system

NASA Technical Reports Server (NTRS)

Raphael, David

1995-01-01

This handbook explicates the hardware and software properties of a time division multiplex system. This system is used to sample analog and digital data. The data is then merged with frame synchronization information to produce a serial pulse coded modulation (PCM) bit stream. Information in this handbook is required by users to design congruous interface and attest effective utilization of this encoder system. Aydin Vector provides all of the components for these systems to Goddard Space Flight Center/Wallops Flight Facility.

14 CFR 460.17 - Verification program.

Code of Federal Regulations, 2011 CFR

2011-01-01

... software in an operational flight environment before allowing any space flight participant on board during a flight. Verification must include flight testing. ... TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with Crew § 460.17 Verification...

14 CFR 460.17 - Verification program.

Code of Federal Regulations, 2010 CFR

2010-01-01

... software in an operational flight environment before allowing any space flight participant on board during a flight. Verification must include flight testing. ... TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with Crew § 460.17 Verification...

14 CFR 460.17 - Verification program.

Code of Federal Regulations, 2012 CFR

2012-01-01

... software in an operational flight environment before allowing any space flight participant on board during a flight. Verification must include flight testing. ... TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with Crew § 460.17 Verification...

14 CFR 460.17 - Verification program.

Code of Federal Regulations, 2013 CFR

2013-01-01

... software in an operational flight environment before allowing any space flight participant on board during a flight. Verification must include flight testing. ... TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with Crew § 460.17 Verification...

14 CFR 460.17 - Verification program.

Code of Federal Regulations, 2014 CFR

2014-01-01

... software in an operational flight environment before allowing any space flight participant on board during a flight. Verification must include flight testing. ... TRANSPORTATION LICENSING HUMAN SPACE FLIGHT REQUIREMENTS Launch and Reentry with Crew § 460.17 Verification...

14 CFR 437.39 - Flight rules.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Flight rules. 437.39 Section 437.39 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Documentation § 437.39 Flight rules. An applicant must provide flight rules as required by § 437.71. ...

14 CFR 437.39 - Flight rules.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Flight rules. 437.39 Section 437.39 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Documentation § 437.39 Flight rules. An applicant must provide flight rules as required by § 437.71. ...

14 CFR 437.39 - Flight rules.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Flight rules. 437.39 Section 437.39 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Documentation § 437.39 Flight rules. An applicant must provide flight rules as required by § 437.71. ...

14 CFR 437.39 - Flight rules.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Flight rules. 437.39 Section 437.39 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Documentation § 437.39 Flight rules. An applicant must provide flight rules as required by § 437.71. ...

14 CFR 437.39 - Flight rules.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Flight rules. 437.39 Section 437.39 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Documentation § 437.39 Flight rules. An applicant must provide flight rules as required by § 437.71. ...

Microgravity Science in Space Flight Gloveboxes

NASA Technical Reports Server (NTRS)

Baugher, Charles; Bennett, Nancy; Cockrell, David; Jex, David; Musick, Barry; Poe, James; Roark, Walter

1998-01-01

Microgravity science studies the influences of gravity on phenomena in fluids, materials processes, combustion, and human cell growth in the low acceleration environment of space flight. During the last decade, the accomplishment of the flight research in the field has evolved into an effective cooperation between the flight crew in the Shuttle and the ground-based investigator using real-time communication via voice and video links. This team structure has led to interactive operations in which the crew performs the experimentation while guided, as necessary, by the science investigator who formulated the investigation and who will subsequently interpret and analyze the data. One of the primary challenges to implementing this interactive research has been the necessity of structuring a means of handling fluids, gases, and hazardous materials in a manned laboratory that exhibits the novelty of weightlessness. Developing clever means of designing experiments in closed vessels is part of the solution- but the space flight requirement for one and two failure-tolerant containment systems leads to serious complications in the physical handling of sample materials. In response to the conflict between the clear advantage of human operation and judgment, versus the necessity to isolate the experiment from the crewmember and the spacecraft environment, the Microgravity Research Program has initiated a series of Gloveboxes in the various manned experiment carriers. These units provide a sealed containment vessel whose interior is under a negative pressure with respect to the ambient environment but is accessible to a crewmember through the glove ports.

Sleep in space

NASA Astrophysics Data System (ADS)

Traon, A. Pavy-le; Roussel, B.

1993-09-01

Manned space flights have shown it is possible to sleep in microgravity. However, some sleep disturbances have been reported which influence performance of the crew and safety of space flight. This paper reviews the main studies of in-flight sleep in animal and man. Most disturbances are related to phase lags due to operational requirements. Factors which can disturb in-flight sleep are analysed: • environmental factors. Some of them are secondary to space flight ergonomics. Conversely, effects of microgravity on light-dark alternance are less known and lead to interesting problems of fundamental research, • psychological factors, especially during long duration flights.

CALCIUM. Photography of the Experiment Ops

NASA Image and Video Library

2014-08-22

ISS040-E-104588 (22 Aug. 2014) --- In the International Space Station’s Rassvet Mini-Research Module 1 (MRM-1), Russian cosmonaut Maxim Suraev, Expedition 40 flight engineer, performs a session of the Calcium experiment, which examines the causes of the loss of bone density that occurs in a weightless environment. For this study, Russian researchers are looking at the solubility of calcium phosphates and human bone samples in water in space.

Williams during blood draw in the JPM during Expedition 22

NASA Image and Video Library

2010-03-12

ISS022-E-091395 (12 March 2010) --- NASA astronaut Jeffrey Williams, Expedition 22 commander, works with test samples in the Human Research Facility 2 (HRF-2) Refrigerated Centrifuge as a part of the Nutritional Status Assessment (Nutrition) experiment in the Columbus laboratory of the International Space Station. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

Application of the Chimera overlapped grid scheme to simulation of Space Shuttle ascent flows

NASA Technical Reports Server (NTRS)

Buning, Pieter G.; Parks, Steven J.; Chan, William M.; Renze, Kevin J.

1992-01-01

Several issues relating to the application of Chimera overlapped grids to complex geometries and flowfields are discussed. These include the addition of geometric components with different grid topologies, gridding for intersecting pieces of geometry, and turbulence modeling in grid overlap regions. Sample results are presented for transonic flow about the Space Shuttle launch vehicle. Comparisons with wind tunnel and flight measured pressures are shown.

InSPACE-3 experiment

NASA Image and Video Library

2013-08-18

ISS036-E-033948 (18 Aug. 2013) --- NASA astronaut Karen Nyberg, Expedition 36 flight engineer, works with new test samples for the Advanced Colloids Experiment, or ACE, housed in the Light Microscopy Module (LMM) inside the Fluids Integrated Rack of the International Space Station?s Destiny laboratory. Results from ACE will help researchers understand how to optimize stabilizers to extend the shelf life of products like laundry detergent, paint, ketchup and even salad dressing.

Microgravity

NASA Image and Video Library

1998-10-10

Dr. Robert Richmond extracts breast cell tissue from one of two liquid nitrogen dewars. NASA's Marshall Space Flight Center (MSFC) is sponsoring research with Bioreactors, rotating wall vessels designed to grow tissue samples in space, to understand how breast cancer works. This ground-based work studies the growth and assembly of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue. Radiation can make the cells cancerous, thus allowing better comparisons of healthy vs. tunourous tissues.

Optical Fiber Assemblies for Space Flight from the NASA Goddard Space Flight Center, Photonics Group

NASA Technical Reports Server (NTRS)

Ott, Melanie N.; Thoma, William Joe; LaRocca, Frank; Chuska, Richard; Switzer, Robert; Day, Lance

2009-01-01

The Photonics Group at NASA Goddard Space Flight Center in the Electrical Engineering Division of the Advanced Engineering and Technologies Directorate has been involved in the design, development, characterization, qualification, manufacturing, integration and anomaly analysis of optical fiber subsystems for over a decade. The group supports a variety of instrumentation across NASA and outside entities that build flight systems. Among the projects currently supported are: The Lunar Reconnaissance Orbiter, the Mars Science Laboratory, the James Webb Space Telescope, the Express Logistics Carrier for the International Space Station and the NASA Electronic Parts. and Packaging Program. A collection of the most pertinent information gathered during project support over the past year in regards to space flight performance of optical fiber components is presented here. The objective is to provide guidance for future space flight designs of instrumentation and communication systems.

Lichens survive in space: results from the 2005 LICHENS experiment.

PubMed

Sancho, Leopoldo G; de la Torre, Rosa; Horneck, Gerda; Ascaso, Carmen; de Los Rios, Asunción; Pintado, Ana; Wierzchos, J; Schuster, M

2007-06-01

This experiment was aimed at establishing, for the first time, the survival capability of lichens exposed to space conditions. In particular, the damaging effect of various wavelengths of extraterrestrial solar UV radiation was studied. The lichens used were the bipolar species Rhizocarpon geographicum and Xanthoria elegans, which were collected above 2000 m in the mountains of central Spain and as endolithic communities inhabiting granites in the Antarctic Dry Valleys. Lichens were exposed to space in the BIOPAN-5 facility of the European Space Agency; BIOPAN-5 is located on the outer shell of the Earth-orbiting FOTON-M2 Russian satellite. The lichen samples were launched from Baikonur by a Soyuz rocket on May 31, 2005, and were returned to Earth after 16 days in space, at which time they were tested for survival. Chlorophyll fluorescence was used for the measurement of photosynthetic parameters. Scanning electron microscopy in back-scattered mode, low temperature scanning electron microscopy, and transmission electron microscopy were used to study the organization and composition of both symbionts. Confocal laser scanning microscopy, in combination with the use of specific fluorescent probes, allowed for the assessment of the physiological state of the cells. All exposed lichens, regardless of the optical filters used, showed nearly the same photosynthetic activity after the flight as measured before the flight. Likewise, the multimicroscopy approach revealed no detectable ultrastructural changes in most of the algal and fungal cells of the lichen thalli, though a greater proportion of cells in the flight samples had compromised membranes, as revealed by the LIVE/DEAD BacLight Bacterial Viability Kit. These findings indicate that most lichenized fungal and algal cells can survive in space after full exposure to massive UV and cosmic radiation, conditions proven to be lethal to bacteria and other microorganisms. The lichen upper cortex seems to provide adequate protection against solar radiation. Moreover, after extreme dehydration induced by high vacuum, the lichens proved to be able to recover, in full, their metabolic activity within 24 hours.

Lichens Survive in Space: Results from the 2005 LICHENS Experiment

NASA Astrophysics Data System (ADS)

Sancho, Leopoldo G.; de la Torre, Rosa; Horneck, Gerda; Ascaso, Carmen; de los Rios, Asunción; Pintado, Ana; Wierzchos, J.; Schuster, M.

2007-06-01

This experiment was aimed at establishing, for the first time, the survival capability of lichens exposed to space conditions. In particular, the damaging effect of various wavelengths of extraterrestrial solar UV radiation was studied. The lichens used were the bipolar species Rhizocarpon geographicum and Xanthoria elegans, which were collected above 2000 m in the mountains of central Spain and as endolithic communities inhabiting granites in the Antarctic Dry Valleys. Lichens were exposed to space in the BIOPAN-5 facility of the European Space Agency; BIOPAN-5 is located on the outer shell of the Earth-orbiting FOTON-M2 Russian satellite. The lichen samples were launched from Baikonur by a Soyuz rocket on May 31, 2005, and were returned to Earth after 16 days in space, at which time they were tested for survival. Chlorophyll fluorescence was used for the measurement of photosynthetic parameters. Scanning electron microscopy in back-scattered mode, low temperature scanning electron microscopy, and transmission electron microscopy were used to study the organization and composition of both symbionts. Confocal laser scanning microscopy, in combination with the use of specific fluorescent probes, allowed for the assessment of the physiological state of the cells. All exposed lichens, regardless of the optical filters used, showed nearly the same photosynthetic activity after the flight as measured before the flight. Likewise, the multimicroscopy approach revealed no detectable ultrastructural changes in most of the algal and fungal cells of the lichen thalli, though a greater proportion of cells in the flight samples had compromised membranes, as revealed by the LIVE/DEAD BacLight Bacterial Viability Kit. These findings indicate that most lichenized fungal and algal cells can survive in space after full exposure to massive UV and cosmic radiation, conditions proven to be lethal to bacteria and other microorganisms. The lichen upper cortex seems to provide adequate protection against solar radiation. Moreover, after extreme dehydration induced by high vacuum, the lichens proved to be able to recover, in full, their metabolic activity within 24 hours.

14 CFR 1214.115 - Standard services.

Code of Federal Regulations, 2011 CFR

2011-01-01

...: commander, pilot and three mission specialists. (e) Orbiter flight planning services. (f) One day of... Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SPACE FLIGHT General Provisions Regarding Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.115 Standard...

STS-80 Space Shuttle Mission Report

NASA Technical Reports Server (NTRS)

Fricke, Robert W., Jr.

1997-01-01

The STS-80 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Reusable Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the eightieth flight of the Space Shuttle Program, the fifty-fifth flight since the return-to-flight, and the twenty-first flight of the Orbiter Columbia (OV-102).

STS-125 Entry flight controllers on console with Flight Director Norman Knight

NASA Image and Video Library

2009-05-24

JSC2009-E-121510 (24 May 2009) --- Flight controllers in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center watch the big screens during the landing of Space Shuttle Atlantis (STS-125) at Edwards Air Force Base in California.

STS-125 Entry flight controllers on console with Flight Director Norman Knight

NASA Image and Video Library

2009-05-24

JSC2009-E-121511 (24 May 2009) --- Flight controllers in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center watch the big screens during the landing of Space Shuttle Atlantis (STS-125) at Edwards Air Force Base in California.

STS-125 Entry flight controllers on console with Flight Director Norman Knight

NASA Image and Video Library

2009-05-24

JSC2009-E-121512 (24 May 2009) --- Flight controllers in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center watch the big screens during the landing of Space Shuttle Atlantis (STS-125) at Edwards Air Force Base in California.

STS-125 Entry flight controllers on console with Flight Director Norman Knight

NASA Image and Video Library

2009-05-24

JSC2009-E-121509 (24 May 2009) --- Flight controllers in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center watch the big screens during the landing of Space Shuttle Atlantis (STS-125) at Edwards Air Force Base in California.