Science.gov

Sample records for species file nos

  1. 77 FR 1061 - Endangered Species; File Nos. 16229 and 16548

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XA881 Endangered Species; File Nos. 16229 and... issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... Zoo, 4401 Zoo ] Parkway, Asheboro, NC 27203 , and the Springfield Science Museum, 21 Edwards Street...

  2. 78 FR 32622 - Endangered Species; File Nos. 17557 and 17273

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ...Notice is hereby given that the National Ocean Service Marine Forensic Lab (NOS Lab) [Responsible Party: M. Katherine Moore], 219 Fort Johnson Road, Charleston, SC 29412 (File No. 17557), and the NOAA Fisheries Northeast Region, Protected Resources Division [Responsible Party: Mary Colligan], 1 Blackburn Drive, Gloucester, MA 01930 (File No. 17273), have applied in due form for permits to take......

  3. 78 FR 23225 - Endangered Species; File Nos. 14759-01 and 16375-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-18

    ... Public Comment'' from the Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File Nos. 14759-01 and 16375-01 from the list of...

  4. 78 FR 50395 - Endangered Species; File Nos. 17557 and 17273

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ...: Permits and Conservation Division, Office of Protected Resources, NMFS, 1315 East-West Highway, Room 13705... NOS Lab and used to support law enforcement actions, research studies (primarily genetics), and... parts and samples would be used to support law enforcement actions, research studies (primarily genetics...

  5. 77 FR 67631 - Endangered Species; File Nos. 17367 and 17364

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-13

    ..., physiology, genetics, health, cryopreservation, and other methods for population conservation, recovery, or... appointment in the following offices: Permits and Conservation Division, Office of Protected Resources, NMFS..., Permits and Conservation Division By email to NMFS.Pr1Comments@noaa.gov (include the File No. in the...

  6. 75 FR 9580 - Marine Mammals and Endangered Species; File Nos. 13544 and 14586

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-03

    ... cetacean species including fin whales (Balaenoptera physalus), sperm whales (Physeter macrocephalus), humpback whales (Megaptera novaengliae), and the North Atlantic right whale (Eubalaena glacialis). The... distribution and possible migrations in the waters of the southeastern Gulf. File No. 14586 is requested under...

  7. 77 FR 21754 - Endangered Species; File Nos. 16526, 16323, 16436, 16422, 16438, 16431, 16507, 16547, 16375...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ..., 16436, 16422, 16438, 16431, 16507, 16547, 16375, 16442, 16482, and 16508 AGENCY: National Marine... 222-226). File No. 16526 The Maine Department of Marine Resources, Gail Wippelhauser, Ph.D... Department of Environmental Protection Marine Fisheries, Tom Savoy (RP), PO Box 719, Old Lyme, CT 06371,...

  8. 78 FR 21112 - Marine Mammals; File Nos. 16992 and 14535

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-09

    ..., Ph.D., Hawaii Institute of Marine Biology, University of Hawaii, P.O. Box 1106, Kailua, HI 96734, has... of Marine Biology in Kaneohe, HI. Researchers would conduct hearing measurements using suction cup... National Oceanic and Atmospheric Administration RIN 0648-XB161 Marine Mammals; File Nos. 16992 and...

  9. 77 FR 12010 - Marine Mammals; File Nos. 1076-1789 and 14502

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XB040 Marine Mammals; File Nos. 1076-1789 and... to Scientific Research Permit Nos. 1076-1789 and 14502. ADDRESSES: The amendment and related... importing of marine mammals (50 CFR part 216). Permit No. 1076-1789: This permit, issued on March 13, 2007...

  10. 76 FR 18725 - Marine Mammals; File Nos. 14330 and 14335

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    .... 14330) the Aleut Community of St. Paul Island, Tribal Government, Ecosystem Conservation Office, St. Paul Island, AK; and (File No. 14335) the Alaska SeaLife Center, Seward, AK. ADDRESSES: The permit... submitted by the Aleut Community of St. Paul Island. The permit has been amended to include...

  11. 75 FR 42689 - Marine Mammals; File Nos. 15498 and 15500

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... Quest Bermuda, Hamilton, Bermuda, to the Brookfield Zoo, Brookfield, IL. File No. 15500 requested the... Island, The Bahamas, and three female captive born bottlenose dolphins from Dolphin Quest Bermuda, Hamilton, Bermuda, to the Georgia Aquarium, Atlanta, Georgia. The requested permits have been issued...

  12. 77 FR 27719 - Marine Mammals; File Nos. 16109 and 15575

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... targeted for research are listed as threatened or endangered: Blue whale (Balaenoptera musculus), fin whale (B. physalus), humpback whale (Megaptera novaeangliae), North Atlantic right whale (Eubalaena... species to be targeted for research are listed as threatened or endangered: Blue whale, fin...

  13. 76 FR 51980 - Atlantic Shipping Company, Inc. v. Di Nos Shipping, Inc.; Notice of Filing of Complaint and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... COMMISSION Atlantic Shipping Company, Inc. v. Di Nos Shipping, Inc.; Notice of Filing of Complaint and...) by Atlantic Shipping Company, Inc., hereinafter ``Complainant,'' against DI Nos Shipping, Inc... Respondent is in violation of the Shipping Act of 1984, 46 U.S.C. 40901 and 40902, by operating as a freight...

  14. 76 FR 37327 - Endangered Species; File No. 16253

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-27

    ... National Oceanic and Atmospheric Administration RIN 0648-XA518 Endangered Species; File No. 16253 AGENCY... requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 16253 from the...

  15. 75 FR 33578 - Endangered Species; File Nos. 14508 and 14655

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-14

    ...Notice is hereby given that Inwater Research Group, Inc. [Permit no. 14508, Principal Investigator, Michael Bresette] Jensen Beach, FL and Jane Provancha [Permit No. 14655], Cape Canaveral, FL have been issued permits to take take green (Chelonia mydas), loggerhead (Caretta caretta), hawksbill (Eretmochelys imbricata), and Kemp's ridley (Lepidochelys kempii) sea turtles for purposes of scientific research.

  16. 78 FR 17640 - Endangered Species; File Nos. 17367 and 17364

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-22

    .... Research would include, but is not limited to, nutrition, physiology, propagation, contaminants, genetics... research animals for studies related to tagging, tracking, behavior, physiology, genetics, health...

  17. 76 FR 15300 - Endangered Species; File Nos. 16266 and 16291

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-21

    ... and educational display of eight captive-bred, non-releaseable adult shortnose sturgeon, as well as.... 1472. Activities would include the acquisition of four captive- ] bred, non-releaseable adult shortnose... the wild, nor would it authorize any release of captive sturgeon into the wild. The permits are...

  18. 76 FR 37065 - Endangered Species; File Nos. 16266 and 16291

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    ..., respectively. Activities include the continued maintenance, transport and educational display of captive-bred... does it authorize any release of captive sturgeon into the wild. The permit is issued for a duration of...

  19. 77 FR 21753 - Endangered Species; File Nos. 13599 and 1614

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... Northeast Region Protected Resources Division [Responsible Party: Mary Colligan], One Blackburn Drive..., NMFS, One Blackburn Drive, Gloucester, MA 01930- 2298; phone (978) 281-9300; fax (978) 281-9394;...

  20. 76 FR 80890 - Endangered Species; File Nos. 13599 and 1614

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-27

    ... samples would be used to support law enforcement actions, research studies (primarily genetics), and...), Commerce. ACTION: Notice; receipt of applications for permit modifications. SUMMARY: Notice is hereby given that the following applicants have applied in due form for modifications to their scientific research...

  1. 77 FR 21750 - Endangered Species; File Nos. 16549 and 17095

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... animal research in laboratory tests of up- and downstream fish passage studies, swimming performance.... Conte Anadromous Fish Research Center, U.S. Geological Survey; Box 796, 1 Migratory Way, Turners Falls... (Battery Park, Manhattan, NY) to River Mile 152 at Troy Dam (Albany, NY). The focus of the monitoring...

  2. 78 FR 6072 - Endangered Species; File No. 17095-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ...'' from the Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov/ , and then selecting File No. 17095-01 from the list of available applications...

  3. 75 FR 67682 - Endangered Species; File No. 15566

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-03

    ... National Oceanic and Atmospheric Administration RIN 0648-XA013 Endangered Species; File No. 15566 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226). The...

  4. 76 FR 22677 - Endangered Species; File No. 14949

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-22

    ... National Oceanic and Atmospheric Administration RIN 0648-XA382 Endangered Species; File No. 14949 AGENCY... under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq..., (2) will not operate to the disadvantage of such endangered or threatened species, and (3) is...

  5. 76 FR 35842 - Endangered Species; File No. 15685

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... Endangered Species; File No. 15685 AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and... subject permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16... endangered and threatened species (50 CFR 222-226). The PIFSC requests a five-year research permit to...

  6. 75 FR 26715 - Endangered Species; File No. 1596

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... Endangered Species; File No. 1596 AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and... July 29, 2009 (74 FR 38585), is requested under the authority of the Endangered Species Act of 1973, as... endangered and threatened species (50 CFR 222-226). Permit No. 1596-02 authorizes the SWFSC to capture...

  7. 76 FR 34967 - Endangered Species; File No. 10027

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    ... National Oceanic and Atmospheric Administration RIN 0648-XA494 Endangered Species; File No. 10027 AGENCY.... 10027, issued on July 30, 2008 (73 FR 44224), is requested under the authority of the Endangered Species..., and exporting of endangered and threatened species (50 CFR 222-226). The permit currently authorizes...

  8. 77 FR 13096 - Endangered Species; File No. 16598

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... National Oceanic and Atmospheric Administration RIN 0648-XB054 Endangered Species; File No. 16598 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... exporting of endangered and threatened species (50 CFR 222-226). The applicant requests a 5-year permit to...

  9. 78 FR 22239 - Endangered Species; File No. 16556

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-15

    ... National Oceanic and Atmospheric Administration RIN 0648-XC037 Endangered Species; File No. 16556 AGENCY... authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts...

  10. 78 FR 50396 - Endangered Species; File No. 17405

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XC519 Endangered Species; File No. 17405 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts 222-226). Permit No...

  11. 75 FR 13256 - Endangered Species; File No. 14176

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XV28 Endangered Species; File No. 14176 AGENCY... permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C... Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov/ , and then...

  12. 76 FR 22877 - Endangered Species; File No. 15566

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-25

    ... National Oceanic and Atmospheric Administration RIN 0648-XA387 Endangered Species; File No. 15566 AGENCY.... The requested permit has been issued under the authority of the Endangered Species Act of 1973, as... of endangered and threatened species (50 CFR parts 222-226). The five-year permit authorizes the...

  13. 78 FR 3882 - Endangered Species; File No. 13543

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-17

    ... National Oceanic and Atmospheric Administration RIN 0648-XJ40 Endangered Species; File No. 13543 AGENCY... requested under the authority of the Endangered Species Act of 1973, as amended (16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species...

  14. 75 FR 13255 - Endangered Species; File No. 15338

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XU83 Endangered Species; File No. 15338 AGENCY... the Endangered Species Act of 1973, as amended (ESA). The Permit application is for the incidental... regulations prohibit the ``taking'' of a species listed as endangered or threatened. The term ``take'' is...

  15. 78 FR 15706 - Endangered Species; File No. 17316

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XC252 Endangered Species; File No. 17316 AGENCY... individual. The requested permit has been issued under the authority of the Endangered Species Act of 1973... exporting of endangered and threatened species (50 CFR parts 222-226). The permit holder is authorized to...

  16. 77 FR 15997 - Endangered Species; File No. 15672

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XB083 Endangered Species; File No. 15672 AGENCY... permit has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S... endangered or threatened species, and (3) is consistent with the purposes and policies set forth in section 2...

  17. 75 FR 4043 - Endangered Species; File No. 14396

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-26

    ... National Oceanic and Atmospheric Administration RIN 0648-XT92 Endangered Species; File No. 14396 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts 222-226). The...

  18. 76 FR 6118 - Endangered Species; File No. 14726

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ...-XA179 Endangered Species; File No. 14726 AGENCY: National Marine Fisheries Service (NMFS), National... subject modification to Permit No. 14726 is requested under the authority of the Endangered Species Act of..., importing, and exporting of endangered and threatened species (50 CFR 222-226). Permit No. 14726, issued on...

  19. 76 FR 66042 - Endangered Species; File No. 1551

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ... National Oceanic and Atmospheric Administration RIN 0648-XA785 Endangered Species; File No. 1551 AGENCY... of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226). The...

  20. 78 FR 57132 - Endangered Species; File No. 16230

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-17

    ... National Oceanic and Atmospheric Administration RIN 0648-XC289 Endangered Species; File No. 16230 AGENCY... incidental take of sea turtles listed under the Endangered Species Act (ESA) associated with commercial and..., importing, and exporting of endangered and threatened species (50 CFR parts 222-226). This permit authorizes...

  1. 78 FR 39258 - Endangered Species; File No. 18069

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-01

    ...-XC725 Endangered Species; File No. 18069 AGENCY: National Marine Fisheries Service (NMFS), National..., importing, and exporting of endangered and threatened species (50 CFR parts 222-226). The applicant requests... Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting [[Page 39259

  2. 77 FR 58812 - Endangered Species; File No. 16733

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... National Oceanic and Atmospheric Administration RIN 0648-XC253 Endangered Species; File No. 16733 AGENCY... permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C... Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa...

  3. 77 FR 37877 - Endangered Species; File No. 17022

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-25

    ... National Oceanic and Atmospheric Administration RIN 0648-XC059 Endangered Species; File No. 17022 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... exporting of endangered and threatened species (50 CFR 222-226). The PIFSC requests a five-year research...

  4. 78 FR 2659 - Endangered Species; File No. 16645

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-14

    ... National Oceanic and Atmospheric Administration RIN 0648-XB152 Endangered Species; File No. 16645 AGENCY... appointment in the following office: Endangered Species Conservation Division, Office of Protected Resources... issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq...

  5. 77 FR 34349 - Endangered Species; File No. 16803

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XC058 Endangered Species; File No. 16803 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226). The...

  6. 77 FR 24686 - Endangered Species; File No. 15634

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-25

    ... National Oceanic and Atmospheric Administration RIN 0648-XA714 Endangered Species; File No. 15634 AGENCY... authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts...

  7. 78 FR 29114 - Endangered Species; File No. 17304

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-17

    ... National Oceanic and Atmospheric Administration RIN 0648-XC667 Endangered Species; File No. 17304 AGENCY... INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of 1973, as... of endangered and threatened species (50 CFR parts 222-226). The applicant requests a five-year...

  8. 78 FR 59657 - Endangered Species; File No. 17304

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ... National Oceanic and Atmospheric Administration RIN 0648-XC667 Endangered Species; File No. 17304 AGENCY... requested permit has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA... endangered and threatened species (50 CFR parts 222-226). Permit No. 17304 authorizes researchers to capture...

  9. 76 FR 48146 - Endangered Species; File No. 1551

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-08

    ... National Oceanic and Atmospheric Administration RIN 0648-XA620 Endangered Species; File No. 1551 AGENCY.... 1551- 02 is requested under the authority of the Endangered Species Act of 1973, as amended (16 U.S.C... the Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps...

  10. 78 FR 51712 - Endangered Species; File No. 16733

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-21

    ... National Oceanic and Atmospheric Administration RIN 0648-XC253 Endangered Species; File No. 16733 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts 222-226). The permit...

  11. 75 FR 26715 - Endangered Species; File No. 10022

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... 0648-XW37 Endangered Species; File No. 10022 AGENCY: National Marine Fisheries Service (NMFS), National... modification has been granted under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U... operate to the disadvantage of such endangered or threatened species, and (3) is consistent with the...

  12. 77 FR 57559 - Endangered Species; File No. 13330

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-18

    ... National Oceanic and Atmospheric Administration RIN 0648-XB144 Endangered Species; File No. 13330 AGENCY... modification has been granted under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U... the disadvantage of such endangered or threatened species, and (3) is consistent with the purposes and...

  13. 77 FR 39220 - Endangered Species; File No. 17183

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-02

    ... National Oceanic and Atmospheric Administration RIN 0648-XC078 Endangered Species; File No. 17183 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... exporting of endangered and threatened species (50 CFR 222-226). The applicant requests a five-year permit...

  14. 76 FR 71515 - Endangered Species; File No. 16174

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ... National Oceanic and Atmospheric Administration RIN 0648-XA826 Endangered Species; File No. 16174 AGENCY... permit has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S... endangered or threatened species, and (3) is consistent with the purposes and policies set forth in section 2...

  15. 76 FR 40699 - Endangered Species; File No. 16229

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XA516 Endangered Species; File No. 16229 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226). The...

  16. 78 FR 31519 - Endangered Species; File No. 13543

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ...-XJ40 Endangered Species; File No. 13543 AGENCY: National Marine Fisheries Service (NMFS), National... modification has been granted under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U... endangered or threatened species, and (3) is consistent with the purposes and policies set forth in section 2...

  17. 76 FR 51945 - Endangered Species; File No. 16548

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XA648 Endangered Species; File No. 16548 AGENCY... subject permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16... endangered and threatened species (50 CFR parts 222-226). The Springfield Science Museum is requesting a...

  18. 75 FR 53278 - Endangered Species; File No. 14759

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... National Oceanic and Atmospheric Administration RIN 0648-XY58 Endangered Species; File No. 14759 AGENCY... individual. The requested permit has been issued under the authority of the Endangered Species Act of 1973... exporting of endangered and threatened species (50 CFR parts 222-226). The applicant is authorized to...

  19. 77 FR 65673 - Endangered Species; File No. 16248

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... National Oceanic and Atmospheric Administration RIN 0648-XC322 Endangered Species; File No. 16248 AGENCY... requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... history and the reasons for the species decline. The proposed project to display endangered cultured...

  20. 78 FR 16255 - Endangered Species; File No. 17022

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-14

    ... National Oceanic and Atmospheric Administration RIN 0648-XC059 Endangered Species; File No. 17022 AGENCY... authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.), and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts...

  1. 75 FR 78227 - Endangered Species; File No. 14400

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-15

    ... National Oceanic and Atmospheric Administration RIN 0648-XA085 Endangered Species; File No. 14400 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts 222-226). This permit...

  2. 77 FR 67341 - Endangered Species; File No. 15809

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XC325 Endangered Species; File No. 15809 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts 222-226). The...

  3. 77 FR 31586 - Endangered Species; File No. 16556

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... National Oceanic and Atmospheric Administration RIN 0648-XC037 Endangered Species; File No. 16556 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... exporting of endangered and threatened species (50 CFR parts 222-226). The NEFSC requests a five-year permit...

  4. 78 FR 17355 - Endangered Species; File No. 17787

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-21

    ... National Oceanic and Atmospheric Administration RIN 0648-XC576 Endangered Species; File No. 17787 AGENCY... INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of 1973, as... of endangered and threatened species (50 CFR parts 222-226). The applicant proposes to gather life...

  5. 75 FR 11863 - Endangered Species; File No. 15135

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XV11 Endangered Species; File No. 15135 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226). The applicant has...

  6. 77 FR 40588 - Endangered Species; File No. 16598

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-10

    ... National Oceanic and Atmospheric Administration RIN 0648-XB054 Endangered Species; File No. 16598 AGENCY... authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts...

  7. 77 FR 72326 - Endangered Species; File No. 17381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... National Oceanic and Atmospheric Administration RIN 0648-XC372 Endangered Species; File No. 17381 AGENCY... INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of 1973, as... of endangered and threatened species (50 CFR 222-226). The applicant requests a 5-year permit to...

  8. 77 FR 30261 - Endangered Species; File No. 16306

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-22

    ... National Oceanic and Atmospheric Administration RIN 0648-XA712 Endangered Species; File No. 16306 AGENCY... requested permit has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA... endangered and threatened species (50 CFR parts 222-226). The Maine Department of Marine Resources was issued...

  9. 78 FR 22517 - Endangered Species; File No. 16549

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-16

    ... National Oceanic and Atmospheric Administration RIN 0648-XB155 Endangered Species; File No. 16549 AGENCY... applicant. The requested permit has been issued under the authority of the Endangered Species Act of 1973... exporting of endangered and threatened species (50 CFR parts 222-226). The Permit Holder is issued a five...

  10. 78 FR 41034 - Endangered Species; File No. 18102

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XC711 Endangered Species; File No. 18102 AGENCY... for a permit pursuant to the Endangered Species Act of 1973, as amended (ESA). The permit application... also available upon written request or by appointment in the following office: Endangered Species...

  11. 76 FR 58471 - Endangered Species; File No. 15634

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ... National Oceanic and Atmospheric Administration RIN 0648-XA714 Endangered Species; File No. 15634 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226). The SWFSC...

  12. 76 FR 58471 - Endangered Species; File No. 16306

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ... National Oceanic and Atmospheric Administration RIN 0648-XA712 Endangered Species; File No. 16306 AGENCY... requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and...

  13. 75 FR 11862 - Endangered Species; File No. 14759

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XV04 Endangered Species; File No. 14759 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.), and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226). The...

  14. 75 FR 13488 - Endangered Species; File No. 14949

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-22

    ... National Oceanic and Atmospheric Administration RIN 0648-XV33 Endangered Species; File No. 14949 AGENCY... permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C...'' from the Features box on the Applications and Permits for Protected Species (APPS) home page, https...

  15. 76 FR 20324 - Endangered Species; File No. 15606

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XA354 Endangered Species; File No. 15606 AGENCY... authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts...

  16. 76 FR 45230 - Endangered Species; File No. 15802

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XA603 Endangered Species; File No. 15802 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... exporting of endangered and threatened species (50 CFR 222-226). The applicant proposes to monitor...

  17. 77 FR 1062 - Endangered Species; File No. 16146

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XA900 Endangered Species; File No. 16146 AGENCY... been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531... good faith, (2) will not operate to the disadvantage of such endangered or threatened species, and (3...

  18. 78 FR 44096 - Endangered Species; File No. 17381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    ... National Oceanic and Atmospheric Administration RIN 0648-XC372 Endangered Species; File No. 17381 AGENCY.... The requested permit has been issued under the authority of the Endangered Species Act of 1973, as... of endangered and threatened species (50 CFR parts 222-226). The above-named individual has been...

  19. 77 FR 58812 - Endangered Species; File No. 17316

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... National Oceanic and Atmospheric Administration RIN 0648-XC252 Endangered Species; File No. 17316 AGENCY.... SUPPLEMENTARY INFORMATION: The subject permit is requested under the authority of the Endangered Species Act of... exporting of endangered and threatened species (50 CFR 222-226). The applicant proposes to gather life...

  20. 77 FR 21751 - Endangered Species; File No. 16645

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XB152 Endangered Species; File No. 16645 AGENCY... Natural Resources (GA DNR) has applied in due form for a permit pursuant to the Endangered Species Act of... following office: Endangered Species Division, Office of Protected Resources, NMFS, 1315 East-West Highway...

  1. 76 FR 44306 - Endangered Species; File No. 16146

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ... National Oceanic and Atmospheric Administration RIN 0648-XA583 Endangered Species; File No. 16146 AGENCY... under the ] authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq... Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then...

  2. 75 FR 71670 - Endangered Species; File No. 15606

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-24

    ... National Oceanic and Atmospheric Administration RIN 0648-XA054 Endangered Species; File No. 15606 AGENCY... under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species...

  3. 76 FR 31597 - Endangered Species; File No. 15614

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    ... National Oceanic and Atmospheric Administration RIN 0648-XA452 Endangered Species; File No. 15614 AGENCY... has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C... endangered or threatened species, and (3) is consistent with the purposes and policies set forth in section 2...

  4. 78 FR 38952 - Endangered Species; File No. 17506

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XC518 Endangered Species; File No. 17506 AGENCY... Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR parts 222-226). Ms...

  5. 78 FR 5779 - Endangered Species; File No. 16248

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-28

    ... National Oceanic and Atmospheric Administration RIN 0648-XC322 Endangered Species; File No. 16248 AGENCY... organization. The requested permit has been issued under the authority of the Endangered Species Act of 1973... exporting of endangered and threatened species (50 CFR parts 222-226). The Riverbanks Zoo and Garden has...

  6. 77 FR 1671 - Endangered Species; File No. 16194

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XA925 Endangered Species; File No. 16194 AGENCY... been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531... good faith, (2) will not operate to the disadvantage of such endangered or threatened species, and (3...

  7. 76 FR 48806 - Endangered Species; File No. 16194

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XA625 Endangered Species; File No. 16194 AGENCY... the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222...

  8. 76 FR 23305 - Endangered Species; File No. 15672

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-26

    ... National Oceanic and Atmospheric Administration RIN 0648-XA385 Endangered Species; File No. 15672 AGENCY... subject permit is requested under the authority of the Endangered Species Act of 1973, as amended (ESA; 16... endangered and threatened species (50 CFR 222-226). Research authorized under Permit No. 15672 would...

  9. 76 FR 77780 - Endangered Species; File No. 10022

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-14

    ... National Oceanic and Atmospheric Administration RIN 0648-XA867 Endangered Species; File No. 10022 AGENCY... the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226)....

  10. 75 FR 61133 - Endangered Species; File No. 14176

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-04

    ... National Oceanic and Atmospheric Administration RIN 0648-XZ31 Endangered Species; File No. 14176 AGENCY... issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened...

  11. 77 FR 10724 - Endangered Species; File No. 16253

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-23

    ... National Oceanic and Atmospheric Administration RIN 0648-XB027 Endangered Species; File No. 16253 AGENCY... permit has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered...

  12. 76 FR 32144 - Endangered Species; File No. 15677

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-03

    ... National Oceanic and Atmospheric Administration RIN 0648-XA467 Endangered Species; File No. 15677 AGENCY... permit has been issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered...

  13. 75 FR 7443 - Endangered Species; File No. 14381

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-19

    ... National Oceanic and Atmospheric Administration RIN 0648-XU26 Endangered Species; File No. 14381 AGENCY... issued under the authority of the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened...

  14. 76 FR 19052 - Endangered Species; File No. 14344

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ... National Oceanic and Atmospheric Administration RIN 0648-XA340 Endangered Species; File No. 14344 AGENCY..., importing, and exporting of endangered and threatened species (50 CFR parts 222-226). The permit authorizes... above-named organization. The requested permit has been issued under the authority of the Endangered...

  15. 78 FR 14078 - Endangered Species; File No. 17405

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-04

    ... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 17405 from the..., 1315 East-West Highway, Room 13705, Silver Spring, MD 20910; phone (301) 427-8401; fax (301) 713-0376; and Southwest Region, NMFS, 501 West Ocean Blvd., Suite 4200, Long Beach, CA 90802-4213; phone...

  16. [COMPARATIVE CHARACTERISTICS OF uNOS-POSITIVE STRUCTURES IN THE CNS OF SOME SPECIES OF CRUSTACEANS].

    PubMed

    Chertok, V M; Kotsyuba, E P

    2015-01-01

    We conducted a comparative study of NO-ergic system in the CNS of 10 species of crustaceans subclass Malacostraca, belonging to orders Stomatopoda and Decapoda, with a common habitat in Ussuri Bay (Sea of Japan). Both similar characteristics and differences in content and distribution of universal NO-synthase (uNOS) were revealed in homologous parts of the brain and ventral nerve cord of the investigated species of crustaceans. We discuss the involvement of nitric oxide in the regulation of physiological functions of decapod crustaceans and its role in the processes of adaptation to the environmental conditions.

  17. 75 FR 43150 - Marine Mammals; File Nos. 10018, 13846, 14451, 14585, 14599, 14122, 14296, and 14353

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... behavioral observations. Four other species of cetaceans (killer whales (Orcinus orca), Pacific white-sided... dalli)) may be incidentally harassed during research. Observed killer whales will be photographed for... killer whales (Pseudorca crassidens) may be incidentally harassed during research. Killer whales and...

  18. 77 FR 13097 - Endangered Species; File Nos. 15661, 10027, and 15685

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... composition, foraging ecology, and migration patterns for green and hawksbill sea turtles in the Northern... focusing on distribution and abundance, ecology, health, and threats to sea turtles at the Palmyra Atoll in... Hawaiian Islands to determine growth rates, health status, stock and population structure, foraging ecology...

  19. 76 FR 23571 - Marine Mammals and Endangered Species; File Nos. 15415 and 14622

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-27

    ...; (2) characterize the aggregations of loggerhead, Kemp's ridley, and hawksbill sea turtles; and (3... during non-linear transect surveys and hand capture loggerhead, Kemp's ridley, and hawksbill sea turtles... given that NMFS has issued two permits to conduct research on marine mammals or sea turtles....

  20. 75 FR 76704 - Endangered Species; File Nos. 13307, 13544, and 14586

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-09

    ..., loggerhead, green, and hawksbill sea turtles in the nearshore waters of Lee County in southwest Florida by... tagging, and sampling (blood, tissue, feces, and lavage) green, hawksbill, and loggerhead sea turtles, a... given that NMFS has issued a permit and two permit modifications to take sea turtles and marine...

  1. 75 FR 16428 - Endangered Species; File Nos. 15112 and 13307-02

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... ridley (Lepidochelys kempii), green (Chelonia mydas), and hawksbill (Eretmochelys imbricata) sea turtles... ridley, 50 green, 10 hawksbill, and 50 leatherback sea turtles caught in commercial fisheries would be.... 13307-02: Dr. Hart is authorized to capture up to 30 green, 20 hawksbill, and 20 loggerhead sea...

  2. 76 FR 58469 - Endangered Species; File Nos. 16526, 16323, 16436, 16422, 16438, 16431, 16507, 16547, 16375...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ..., 16436, 16422, 16438, 16431, 16507, 16547, 16375, 16442, 16482, and 16508. AGENCY: National Marine.... Gail Wippelhauser, PhD, of the Maine Department of Marine Resources, 21 State House Station, Augusta... telemetry tag. Tom Savoy of the Connecticut Department of Environmental Protection, Marine Fisheries,...

  3. 78 FR 38013 - Endangered Species; File No. 15661

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-25

    ... authority of the Endangered Species Act of 1973, as amended (16 U.S.C. 1531 et seq.) and the regulations governing the taking, importing, and exporting of endangered and threatened species (50 CFR 222-226). Permit... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XA425 Endangered...

  4. 78 FR 32623 - Endangered Species; File No. 17452

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ... permit to take shortnose (Acipenser brevirostrum) and Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus... shortnose sturgeon. For each species, up to 20 adult and juvenile animals would be collected annually using gill nets. Once captured, sturgeon would be measured, weighed, tissue sampled, and passive...

  5. 78 FR 66901 - Endangered Species; File No. 16482-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-07

    ... Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa..., MD 20910; phone (301) 427-8401; fax (301) 713-0376; and Southeast Region, NMFS, 263 13th Avenue South, Saint Petersburg, Florida 33701; phone (727) 824-5312; fax (727) 824-5309. Written comments on...

  6. 76 FR 45517 - Endangered Species; File No. 13330-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-29

    ... unchanged from the original permit: to collect data on the biology, distribution and abundance of the endangered smalltooth sawfish to facilitate recovery of the species. Sampling with the goal of taking 45... transmitting (PAT) tags, and Smart Position Only Transmitting (SPOT) tags. Sampling methods also include...

  7. iNOS null MRL+/+ mice show attenuation of trichloroethene-mediated autoimmunity: contribution of reactive nitrogen species and lipid-derived reactive aldehydes.

    PubMed

    Wang, Gangduo; Wakamiya, Maki; Wang, Jianling; Ansari, G A S; Firoze Khan, M

    2015-12-01

    Earlier studies from our laboratory in MRL+/+ mice suggest that free radicals, especially overproduction of reactive nitrogen species (RNS) and lipid-derived reactive aldehydes (LDRAs), are associated with trichloroethene (TCE)-mediated autoimmune response. The current study was undertaken to further assess the contribution of RNS and LDRAs in TCE-mediated autoimmunity by using iNOS-null MRL+/+ mice. iNOS-null MRL+/+ mice were obtained by backcrossing iNOS-null mice (B6.129P2-Nos2(tm1Lau)/J) to MRL +/+ mice. Female MRL+/+ and iNOS-null MRL+/+ mice were given TCE (10 mmol/kg, i.p., every 4(th) day) for 6 weeks; their respective controls received corn oil only. TCE exposure led to significantly increased iNOS mRNA in livers, iNOS protein in livers and sera, increased nitrotyrosine (NT) formation in both livers and sera, induction of MDA-/HNE-protein adducts in livers and their respective antibodies in sera along with significant increases in serum antinuclear antibodies (ANA) and anti-dsDNA in MRL+/+ mice. Even though in iNOS-null MRL+/+ mice, the iNOS and NT levels were negligible in both TCE-treated and untreated groups, TCE treatment still led to significant increases in MDA-/HNE-protein adducts and their respective antibodies along with increases in serum ANA and anti-dsDNA compared to controls. Most remarkably, the increases in serum ANA and anti-dsDNA induced by TCE in the iNOS-null MRL+/+ mice were significantly less pronounced compared to that in MRL+/+ mice. Our results provide further evidence that both RNS and LDRAs contribute to TCE-induced autoimmunity in MRL+/+ mice, and iNOS deficiency attenuates this autoimmune response.

  8. iNOS null MRL+/+ mice show attenuation of trichloroethene-mediated autoimmunity: contribution of reactive nitrogen species and lipid-derived reactive aldehydes

    PubMed Central

    Wang, Gangduo; Wakamiya, Maki; Wang, Jianling; Ansari, G.A.S.; Khan, M. Firoze

    2015-01-01

    Earlier studies from our laboratory in MRL+/+ mice suggest that free radicals, especially overproduction of reactive nitrogen species (RNS) and lipid-derived reactive aldehydes (LDRAs), are associated with trichloroethene (TCE)-mediated autoimmune response. The current study was undertaken to further assess the contribution of RNS and LDRAs in TCE-mediated autoimmunity by using iNOS-null MRL+/+ mice. iNOS-null MRL+/+ mice were obtained by backcrossing iNOS-null mice (B6.129P2-Nos2tm1Lau/J) to MRL +/+ mice. Female MRL+/+ and iNOS-null MRL+/+ mice were given TCE (10 mmol/kg, i.p., every 4th day) for 6 weeks; their respective controls received corn oil only. TCE exposure led to significantly increased iNOS mRNA in livers, iNOS protein in livers and sera, increased nitrotyrosine (NT) formation in both livers and sera, induction of MDA-/HNE-protein adducts in livers and their respective antibodies in sera along with significant increases in serum antinuclear antibodies (ANA) and anti-dsDNA in MRL+/+ mice. Even though in iNOS-null MRL+/+ mice, the iNOS and NT levels were negligible in both TCE-treated and untreated groups, TCE treatment still led to significant increases in MDA-/HNE-protein adducts and their respective antibodies along with increases in serum ANA and anti-dsDNA compared to controls. Most remarkably, the increases in serum ANA and anti-dsDNA induced by TCE in the iNOS-null MRL+/+ mice were significantly less pronounced compared to that in MRL+/+ mice. Our results provide further evidence that both RNS and LDRAs contribute to TCE-induced autoimmunity in MRL+/+ mice, and iNOS deficiency attenuates this autoimmune response. PMID:26472195

  9. Role of reactive oxygen species in the signalling cascade of cyclosporine A-mediated up-regulation of eNOS in vascular endothelial cells

    PubMed Central

    López-Ongil, S; Hernández-Perera, O; Navarro-Antolín, J; Pérez de Lema, G; Rodríguez-Puyol, M; Lamas, S; Rodríguez-Puyol, D

    1998-01-01

    Cyclosporine A (CsA) increases eNOS mRNA expression in bovine cultured aortic endothelial cells (BAEC). As some effects of CsA may be mediated by reactive oxygen species (ROS), present experiments were devoted to test the hypothesis that the CsA-induced eNOS up-regulation could be dependent on an increased synthesis of ROS.CsA induced a dose-dependent increase of ROS synthesis, with the two fluorescent probes used, DHR123 (CsA 1 μM: 305±7% over control) and H2DCFDA (CsA 1 μM: 178±6% over control).Two ROS generating systems, xanthine plus xanthine oxidase (XXO) and glucose oxidase (GO), increased the expression of eNOS mRNA in BAEC, an effect which was maximal after 8 h of incubation (XXO: 168±21% of control values. GO: 208±18% of control values). The ROS-dependent increased eNOS mRNA expression was followed by an increase in eNOS activity.The effect of CsA on eNOS mRNA expression was abrogated by catalase, and superoxide dismutase (SOD). In contrast, the antioxidant PDTC augmented eNOS mRNA expression, both in basal conditions and in the presence of CsA.The potential participation of the transcription factor AP-1 was explored. Electrophoretic mobility shift assays were consistent with an increase in AP-1 DNA-binding activity in BAEC treated with CsA or glucose oxidase.The present results support a role for ROS, particularly superoxide anion and hydrogen peroxide, as mediators of the CsA-induced eNOS mRNA up-regulation. Furthermore, they situate ROS as potential regulators of gene expression in endothelial cells, both in physiological and pathophysiological situations. PMID:9647467

  10. 76 FR 30309 - Marine Mammals and Endangered Species; File Nos. 14245, 1596-03, and 14726-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    .../sonic tag/GPS unit, or VHR/TDR/sonic tag/GPS/video camera system to leatherback sea turtles. Animals... directly attach a tag to the animal's medial ridge in place of the previously authorized harness attachment... behavior in the Pacific Ocean between their foraging areas and nesting beaches. The modification is valid...

  11. 75 FR 61133 - Marine Mammals and Endangered Species; File Nos. 808-1735, 14233, 14506, 14603, and 14726

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-04

    ... research on green, hawksbill, Kemp's ridley, loggerhead, and leatherback sea turtles had been submitted by... ridley, hawksbill, and leatherback sea turtles. A subset of green sea turtles may have a transmitter... amendment to conduct research ] on marine mammals or sea turtles. See SUPPLEMENTARY INFORMATION...

  12. Biocorrosion of Endodontic Files through the Action of Two Species of Sulfate-reducing Bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis.

    PubMed

    Heggendorn, Fabiano Luiz; Gonçalves, Lucio Souza; Dias, Eliane Pedra; de Oliveira Freitas Lione, Viviane; Lutterbach, Márcia Teresa Soares

    2015-08-01

    This study assessed the biocorrosive capacity of two bacteria: Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis on endodontic files, as a preliminary step in the development of a biopharmaceutical, to facilitate the removal of endodontic file fragments from root canals. In the first stage, the corrosive potential of the artificial saliva medium (ASM), modified Postgate E medium (MPEM), 2.5 % sodium hypochlorite (NaOCl) solution and white medium (WM), without the inoculation of bacteria was assessed by immersion assays. In the second stage, test samples were inoculated with the two species of sulphur-reducing bacteria (SRB) on ASM and modified artificial saliva medium (MASM). In the third stage, test samples were inoculated with the same species on MPEM, ASM and MASM. All test samples were viewed under an infinite focus Alicona microscope. No test sample became corroded when immersed only in media, without bacteria. With the exception of one test sample between those inoculated with bacteria in ASM and MASM, there was no evidence of corrosion. Fifty percent of the test samples demonstrated a greater intensity of biocorrosion when compared with the initial assays. Desulfovibrio desulfuricans and D. fairfieldensis are capable of promoting biocorrosion of the steel constituent of endodontic files. This study describes the initial development of a biopharmaceutical to facilitate the removal of endodontic file fragments from root canals, which can be successfully implicated in endodontic therapy in order to avoiding parendodontic surgery or even tooth loss in such events.

  13. Permanent-File-Validation Utility Computer Program

    NASA Technical Reports Server (NTRS)

    Derry, Stephen D.

    1988-01-01

    Errors in files detected and corrected during operation. Permanent File Validation (PFVAL) utility computer program provides CDC CYBER NOS sites with mechanism to verify integrity of permanent file base. Locates and identifies permanent file errors in Mass Storage Table (MST) and Track Reservation Table (TRT), in permanent file catalog entries (PFC's) in permit sectors, and in disk sector linkage. All detected errors written to listing file and system and job day files. Program operates by reading system tables , catalog track, permit sectors, and disk linkage bytes to vaidate expected and actual file linkages. Used extensively to identify and locate errors in permanent files and enable online correction, reducing computer-system downtime.

  14. SNPAAMapperT2K: A genome-wide SNP downstream analysis and annotation pipeline for species annotated with NCBI.tbl data files.

    PubMed

    Bai, Yongsheng

    2014-01-01

    SNPAAMapper, a genome-wide SNP downstream analysis and annotation pipeline, was designed to classify detected variants according to genomic regions and report the mutation class by processing whole-genome and/or whole-exome sequencing data. A widely used sequence and data annotation table format "knownGene.txt" has not yet been created for many popular model organisms (e.g. Arabidopsis). Instead, NCBI .tbl annotation format files are provided for these species. Therefore, it is of interest to describe SNPAAMapperT2K, a genome-wide SNP downstream analysis and annotation pipeline for species annotated with NCBI .tbl data files (e.g. Arabidopsis). The pipeline is tested with a deeply sequenced Arabidopsis thaliana strain (Seattle-0). The SNPAAMapperT2K can also annotate and report SNP classes for other species, whose chromosome files are annotated as NCBI .tbl format, but do not have their annotated knownGene.txt files available. Perl scripts and required input files are available on the web at http://isu.indstate.edu/ybai2/SNPAAMapperT2K.

  15. Langley VEDIT for NOS/VE usage manual

    NASA Technical Reports Server (NTRS)

    Heaney, Margaret A.

    1987-01-01

    The use of the VEDIT editor on the CDC Network Operating System/Virtual Environment (NOS/VE) is discussed. The VEDIT, a utility, allows a user to edit files line by line (line mode). How to access and the use of VEDIT are explained. The parameters and the format of the individual commands are defined. Examples are included.

  16. 15. Dry Dock No. 4. Longitudinal Section. Subdivision Nos. I ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Dry Dock No. 4. Longitudinal Section. Subdivision Nos. I and II (Frederic R. Harris, Inc., January 10, 1941). In Files of Cushman & Wakefield, Building no. 501, Philadelphia Naval Business Center. - Naval Base Philadelphia-Philadelphia Naval Shipyard, Dry Dock No. 4, Broad Street south of Government Avenue, Philadelphia, Philadelphia County, PA

  17. Service building. Cross section thru dry dock nos. 4 & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Service building. Cross section thru dry dock nos. 4 & 5 showing service bldg & 20-75-150 ton cranes (dry dock associates, May 23, 1941). In files of Cushman & Wakefield, building no. 501, Philadelphia Naval Business Center. - Naval Base Philadelphia-Philadelphia Naval Shipyard, Service Building, Dry Docks No. 4 & 5, League Island, Philadelphia, Philadelphia County, PA

  18. 16. Dry Dock No. 4. Longitudinal Section. Subdivision Nos. III ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Dry Dock No. 4. Longitudinal Section. Subdivision Nos. III and IV (Frederic R. Harris, Inc., January 10, 1941). In Files of Cushman & Wakefield, Building no. 501, Philadelphia Naval Business Center. - Naval Base Philadelphia-Philadelphia Naval Shipyard, Dry Dock No. 4, Broad Street south of Government Avenue, Philadelphia, Philadelphia County, PA

  19. mNos2 Deletion and Human NOS2 Replacement in Alzheimer Disease Models

    PubMed Central

    Colton, Carol A.; Wilson, Joan G.; Everhart, Angela; Wilcock, Donna M.; Puoliväli, Jukka; Heikkinen, Taneli; Oksman, Juho; Jääskeläinen, Olli; Lehtimäki, Kimmo; Laitinen, Teemu; Vartiainen, Nina; Vitek, Michael P.

    2014-01-01

    Abstract Understanding the pathophysiologic mechanisms underlying Alzheimer disease relies on knowledge of disease onset and the sequence of development of brain pathologies. We present a comprehensive analysis of early and progressive changes in a mouse model that demonstrates a full spectrum of characteristic Alzheimer disease–like pathologies. This model demonstrates an altered immune redox state reminiscent of the human disease and capitalizes on data indicating critical differences between human and mouse immune responses, particularly in nitric oxide levels produced by immune activation of the NOS2 gene. Using the APPSwDI+/+/mNos2−/− (CVN-AD) mouse strain, we show a sequence of pathologic events leading to neurodegeneration,which include pathologically hyperphosphorylated tau in the perforant pathway at 6 weeks of age progressing to insoluble tau, early appearance of β-amyloid peptides in perivascular deposits around blood vessels in brain regions known to be vulnerable to Alzheimer disease, and progression to damage and overt loss in select vulnerable neuronal populations in these regions. The role of species differences between hNOS2 and mNos2 was supported by generating mice in which the human NOS2 gene replaced mNos2. When crossed with CVN-AD mice, pathologic characteristics of this new strain (APPSwDI+/−/HuNOS2tg+/+/mNos2−/−) mimicked the pathologic phenotypes found in the CVN-AD strain. PMID:25003233

  20. mNos2 deletion and human NOS2 replacement in Alzheimer disease models.

    PubMed

    Colton, Carol A; Wilson, Joan G; Everhart, Angela; Wilcock, Donna M; Puoliväli, Jukka; Heikkinen, Taneli; Oksman, Juho; Jääskeläinen, Olli; Lehtimäki, Kimmo; Laitinen, Teemu; Vartiainen, Nina; Vitek, Michael P

    2014-08-01

    Understanding the pathophysiologic mechanisms underlying Alzheimer disease relies on knowledge of disease onset and the sequence of development of brain pathologies. We present a comprehensive analysis of early and progressive changes in a mouse model that demonstrates a full spectrum of characteristic Alzheimer disease-like pathologies. This model demonstrates an altered immune redox state reminiscent of the human disease and capitalizes on data indicating critical differences between human and mouse immune responses, particularly in nitric oxide levels produced by immune activation of the NOS2 gene. Using the APPSwDI(+)/(+)mNos2(-/-) (CVN-AD) mouse strain, we show a sequence of pathologic events leading to neurodegeneration,which include pathologically hyperphosphorylated tau in the perforant pathway at 6 weeks of age progressing to insoluble tau, early appearance of β-amyloid peptides in perivascular deposits around blood vessels in brain regions known to be vulnerable to Alzheimer disease, and progression to damage and overt loss in select vulnerable neuronal populations in these regions. The role of species differences between hNOS2 and mNos2 was supported by generating mice in which the human NOS2 gene replaced mNos2. When crossed with CVN-AD mice, pathologic characteristics of this new strain (APPSwDI(+)/(-)/HuNOS2(tg+)/(+)/mNos2(-/-)) mimicked the pathologic phenotypes found in the CVN-AD strain.

  1. The NOS Challenge

    ERIC Educational Resources Information Center

    Quigley, Cassie; Buck, Gayle; Akerson, Valarie

    2011-01-01

    "The picture of a scientist is me!" exclaims first grader Kendra during a nature of science (NOS) lesson. She drew a picture of a scientist and explained that she was going to be a scientist when she grew up because she "loved to observe like a scientist." Kendra's experience was a part of a 30-day unit designed specifically for first graders.…

  2. The NOS Challenge

    ERIC Educational Resources Information Center

    Quigley, Cassie; Buck, Gayle; Akerson, Valarie

    2011-01-01

    "The picture of a scientist is me!" exclaims first grader Kendra during a nature of science (NOS) lesson. She drew a picture of a scientist and explained that she was going to be a scientist when she grew up because she "loved to observe like a scientist." Kendra's experience was a part of a 30-day unit designed specifically for first graders.…

  3. 77 FR 24481 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-24

    .... Description: Revisions to Attachments H and T to Implement Balanced Portfolio Transfers to be effective 6/1... General Electric Company. Description: Annual Informational Filing on Operational Penalty Assessments and... Penalty Assessments and Distributions in Accordance with Order Nos. 890 and 890-A. Filed Date:...

  4. Expression of nNOS in the human larynx.

    PubMed

    Şelaru, Mircea; Rusu, Mugurel Constantin; Jianu, Adelina Maria

    2015-09-01

    Although intrinsic laryngeal neurons and ganglia have been studied in various species, they have been overlooked in humans. We aimed to investigate the presence of intrinsic laryngeal neurons in humans and, if present, to analyze their neuronal nitric oxide synthase (nNOS) expression. An immunohistochemical study using anti-nNOS antibodies was performed on samples obtained from four cadavers. Intrinsic laryngeal nNOS+ neurons were assessed in the submucosal layer, but nNOS+ nerves were found in all histological layers of the larynx. nNOS expression was also found in striated muscle fibers of larynx. This might reveal the anatomical basis of an upwards extension of the nonadrenergic noncholinergic system in human airways, but further experiments are needed to assess an exact role of NO influence on neural transmission and muscular functions of human larynx.

  5. Nitric oxide synthase (NOS) in the Japanese fireflies Luciola lateralis and Luciola cruciata.

    PubMed

    Ohtsuki, Hajime; Yokoyama, Jun; Ohba, Nobuyoshi; Ohmiya, Yoshihiro; Kawata, Masakado

    2008-12-01

    Species-specific flash patterns in firefly species are important for the investigation of the evolution of Lampyridae. Since nitric oxide synthase (NOS) is one of the key enzymes controlling flash patterns, we determined the cDNA sequences of NOS in the Japanese fireflies Luciola lateralis and L. cruciata. The identity of the NOS sequences was very high between these 2 species. Firefly NOS also exhibited a high identity with those of other insect species, and the cofactor-binding domains were particularly well conserved. Many negatively selected sites were detected throughout the NOS sequences; however, no positive selection was detected. The phylogenetic relationship of insect NOS was different from that of the general classification system, although the lineages corresponded to the major recognized taxonomic groups.

  6. Endothelial NOS (NOS3) impairs myocardial function in developing sepsis.

    PubMed

    van de Sandt, Annette M; Windler, Rainer; Gödecke, Axel; Ohlig, Jan; Zander, Simone; Reinartz, Michael; Graf, Jürgen; van Faassen, Ernst E; Rassaf, Tienush; Schrader, Jürgen; Kelm, Malte; Merx, Marc W

    2013-03-01

    Endothelial nitric oxide synthase (NOS)3-derived nitric oxide (NO) modulates inotropic response and diastolic interval for optimal cardiac performance under non-inflammatory conditions. In sepsis, excessive NO production plays a key role in severe hypotension and myocardial dysfunction. We aimed to determine the role of NOS3 on myocardial performance, NO production, and time course of sepsis development. NOS3(-/-) and C57BL/6 wildtype mice were rendered septic by cecum ligation and puncture (CLP). Cardiac function was analyzed by serial echocardiography, in vivo pressure and isolated heart measurements. Cardiac output (CO) increased to 160 % of baseline at 10 h after sepsis induction followed by a decline to 63 % of baseline after 18 h in wildtype mice. CO was unaltered in septic NOS3(-/-) mice. Despite the hyperdynamic state, cardiac function and mean arterial pressure were impaired in septic wildtype as early as 6 h post CLP. At 12 h, cardiac function in septic wildtype was refractory to catecholamines in vivo and respective isolated hearts showed impaired pressure development and limited coronary flow reserve. Hemodynamics remained stable in NOS3(-/-) mice leading to significant survival benefit. Unselective NOS inhibition in septic NOS3(-/-) mice diminished this survival benefit. Plasma NO( x )- and local myocardial NO( x )- and NO levels (via NO spin trapping) demonstrated enhanced NO( x )- and bioactive NO levels in septic wildtype as compared to NOS3(-/-) mice. Significant contribution by inducible NOS (NOS2) during this early phase of sepsis was excluded. Our data suggest that NOS3 relevantly contributes to bioactive NO pool in developing sepsis resulting in impaired cardiac contractility.

  7. 77 FR 51985 - Archon Energy 1, Inc.; Notice of Intent To File License Application, Filing of Pre-Application...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-28

    ... of Pre-Application Document, and Approving Use of the Traditional Licensing Process a. Type of Filing..., pursuant to section 7 of the Endangered Species Act. m. Archon filed a Pre-Application Document (PAD)...

  8. 76 FR 20971 - Free Flow Power Corporation; Notice of Intent To File License Application, Filing of Pre...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-14

    ..., Filing of Pre-Application Document, and Approving Use of the Traditional Licensing Process a. Type of... Species Act and section 106 of the National Historic Preservation Act. m. Free Flow Power filed a...

  9. 75 FR 37389 - Marine Mammals; File Nos. 14628 and 15471

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-29

    ... purposes:National Museum of Natural History, Smithsonian Institution (Charles W. Potter, Responsible Party... Museum of Natural History (NMNH) is requesting authorization to collect, import, export, possess,...

  10. 77 FR 64959 - Marine Mammals; File Nos. 15777 and 17670

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-24

    ... seals (Halichoerus grypus), harp seals (Pagophilus groenlandicus), and hooded seals (Cystophora cristata..., hair, swab samples, vibrissae), and attachment of telemetry devices. Up to 200 harp seals, 50 hooded... seals, 8 harbor seals, 2 harp seals, and 2 hooded seals per year. Permission is also sought to import...

  11. 76 FR 51001 - Marine Mammals; File Nos. 16109 and 15575

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE... appointment in the following offices: Permits, Conservation and Education Division, Office of Protected... the Chief, Permits, Conservation and Education Division, at the address listed above. Comments may...

  12. 75 FR 23242 - Marine Mammals; File Nos. 15498 and 15500

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-03

    ... female captive born bottlenose dolphin from Dolphin Quest Bermuda, Hamilton, Bermuda, to the Brookfield... captive born bottlenose dolphins from Dolphin Quest Bermuda, Hamilton, Bermuda, to its facility in...

  13. 78 FR 60852 - Marine Mammals; File Nos. 16239 and 17312

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    ... issued to Dan Engelhaupt, Ph.D., HDR EOC, 5700 Lake Wright Drive, Norfolk, VA 23502- 1859, and Scripps Institution of Oceanography [Responsible Party: John Hildebrand, Ph.D.], University of California,...

  14. Contribution of the nos-pdt operon to virulence phenotypes in methicillin-sensitive Staphylococcus aureus.

    PubMed

    Sapp, April M; Mogen, Austin B; Almand, Erin A; Rivera, Frances E; Shaw, Lindsey N; Richardson, Anthony R; Rice, Kelly C

    2014-01-01

    Nitric oxide (NO) is emerging as an important regulator of bacterial stress resistance, biofilm development, and virulence. One potential source of endogenous NO production in the pathogen Staphylococcus aureus is its NO-synthase (saNOS) enzyme, encoded by the nos gene. Although a role for saNOS in oxidative stress resistance, antibiotic resistance, and virulence has been recently-described, insights into the regulation of nos expression and saNOS enzyme activity remain elusive. To this end, transcriptional analysis of the nos gene in S. aureus strain UAMS-1 was performed, which revealed that nos expression increases during low-oxygen growth and is growth-phase dependent. Furthermore, nos is co-transcribed with a downstream gene, designated pdt, which encodes a prephenate dehydratase (PDT) enzyme involved in phenylalanine biosynthesis. Deletion of pdt significantly impaired the ability of UAMS-1 to grow in chemically-defined media lacking phenylalanine, confirming the function of this enzyme. Bioinformatics analysis revealed that the operon organization of nos-pdt appears to be unique to the staphylococci. As described for other S. aureus nos mutants, inactivation of nos in UAMS-1 conferred sensitivity to oxidative stress, while deletion of pdt did not affect this phenotype. The nos mutant also displayed reduced virulence in a murine sepsis infection model, and increased carotenoid pigmentation when cultured on agar plates, both previously-undescribed nos mutant phenotypes. Utilizing the fluorescent stain 4-Amino-5-Methylamino-2',7'-Difluorofluorescein (DAF-FM) diacetate, decreased levels of intracellular NO/reactive nitrogen species (RNS) were detected in the nos mutant on agar plates. These results reinforce the important role of saNOS in S. aureus physiology and virulence, and have identified an in vitro growth condition under which saNOS activity appears to be upregulated. However, the significance of the operon organization of nos-pdt and potential

  15. Therapeutic effect of enhancing endothelial nitric oxide synthase (eNOS) expression and preventing eNOS uncoupling

    PubMed Central

    Förstermann, Ulrich; Li, Huige

    2011-01-01

    Nitric oxide (NO) produced by the endothelium is an important protective molecule in the vasculature. It is generated by the enzyme endothelial NO synthase (eNOS). Similar to all NOS isoforms, functional eNOS transfers electrons from nicotinamide adenine dinucleotide phosphate (NADPH), via the flavins flavin adenine dinucleotide and flavin mononucleotide in the carboxy-terminal reductase domain, to the heme in the amino-terminal oxygenase domain. Here, the substrate L-arginine is oxidized to L-citrulline and NO. Cardiovascular risk factors such as diabetes mellitus, hypertension, hypercholesterolaemia or cigarette smoking reduce bioactive NO. These risk factors lead to an enhanced production of reactive oxygen species (ROS) in the vessel wall. NADPH oxidases represent major sources of this ROS and have been found upregulated in the presence of cardiovascular risk factors. NADPH-oxidase-derived superoxide avidly reacts with eNOS-derived NO to form peroxynitrite (ONOO-). The essential NOS cofactor (6R-)5,6,7,8-tetrahydrobiopterin (BH4) is highly sensitive to oxidation by this ONOO-. In BH4 deficiency, oxygen reduction uncouples from NO synthesis, thereby converting NOS to a superoxide-producing enzyme. Among conventional drugs, compounds interfering with the renin-angiotensin-aldosterone system and statins can reduce vascular oxidative stress and increase bioactive NO. In recent years, we have identified a number of small molecules that have the potential to prevent eNOS uncoupling and, at the same time, enhance eNOS expression. These include the protein kinase C inhibitor midostaurin, the pentacyclic triterpenoids ursolic acid and betulinic acid, the eNOS enhancing compounds AVE9488 and AVE3085, and the polyphenolic phytoalexin trans-resveratrol. Such compounds enhance NO production from eNOS also under pathophysiological conditions and may thus have therapeutic potential. PMID:21198553

  16. Computer files.

    PubMed

    Malik, M

    1995-02-01

    From what has been said, several recommendations can be made for users of small personal computers regardless of which operating system they use. If your computer has a large hard disk not specially required by any single application, organize the disk into a small number of volumes. You will then be using the computer as if it had several smaller disks, which will help you to create a logical file structure. The size of individual volumes has to be selected carefully with respect to the files kept in each volume. Otherwise, it may be that you will have too much space in one volume and not enough in another. In each volume, organize the structure of directories and subdirectories logically so that they correspond to the logic of your file content. Be aware of the fact that the directories suggested as default when installing new software are often not the optimum. For instance, it is better to put different graphics packages under a common subdirectory rather than to install them at the same level as all other packages including statistics, text processors, etc. Create a special directory for each task you use the computer. Note that it is a bad practice to keep many different and logically unsorted files in the root directory of any of your volumes. Only system and important service files should be kept there. Although any file may be written all over the disk, access to it will be faster if it is written over the minimum number of cylinders. From time to time, use special programs that reorganize your files in this way.(ABSTRACT TRUNCATED AT 250 WORDS)

  17. Compress Your Files

    ERIC Educational Resources Information Center

    Branzburg, Jeffrey

    2005-01-01

    File compression enables data to be squeezed together, greatly reducing file size. Why would someone want to do this? Reducing file size enables the sending and receiving of files over the Internet more quickly, the ability to store more files on the hard drive, and the ability pack many related files into one archive (for example, all files…

  18. Compress Your Files

    ERIC Educational Resources Information Center

    Branzburg, Jeffrey

    2005-01-01

    File compression enables data to be squeezed together, greatly reducing file size. Why would someone want to do this? Reducing file size enables the sending and receiving of files over the Internet more quickly, the ability to store more files on the hard drive, and the ability pack many related files into one archive (for example, all files…

  19. 75 FR 39915 - Marine Mammals; File No. 15483

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-13

    ... National Oceanic and Atmospheric Administration RIN 0648-XX23 Marine Mammals; File No. 15483 AGENCY... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15483 from the... include the File No. in the subject line of the email comment. Those individuals requesting a...

  20. Association analysis of nitric oxide synthases: NOS1, NOS2A and NOS3 genes, with multiple sclerosis.

    PubMed

    AlFadhli, Suad; Mohammed, Eiman M A; Al Shubaili, Asmahan

    2013-07-01

    Multiple sclerosis (MS) is a chronic inflammatory autoimmune disorder of the central nervous system. To explore the genetic basis of three nitric oxide synthase (NOS) genes: NOS1, NOS2A and NOS3, with susceptibility to MS. A total of 122 MS patients and 118 healthy controls screened for NOS1 (rs2682826, rs41279104), NOS2A (CCTTT)n/(TAAA)n and NOS3 (rs1800783, rs1800779, rs2070744, 27bpVNTR) markers, using TaqMan®SNP Genotyping Assays and fragment analysis were enrolled in this study. QRT-PCR and ELISA were used to analyse the expression of NOS3 mRNA and Nitric Oxide (NO) levels. Two NOS3 markers were associated with susceptibility to MS and early disease development. The NOS3 rs1800779 G-allele (p = 0.04) and GG-genotype (p = 0.02) showed association with susceptibility to MS. Short NOS2 (CCTTT)n (p = 0.03) and short/long repeat (p = 0.04) genotypes also showed associations with MS. These associations were intensified by sub-division of patients into Kuwaiti Arabs and Persians (p < 0.05). The NOS3-27 bp-VNTR a-allele was associated with early MS disease onset ≤26 years (p = 0.04). The NOS3-27 bp-VNTR a/b-genotype resulted in 23% lower NO production and the NOS3-rs1800779 AA-genotype resulted in lower NOS3 expression. Haplotypes obtained from NOS2A and NOS3 showed increased susceptibility to MS. NOS1 showed no significant association with MS. This study provides evidence for the association between selected NOS2 and NOS3 markers and MS susceptibility.

  1. 75 FR 9201 - DCP Raptor Pipeline, LLC; Notice of Compliance Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-01

    ... Energy Regulatory Commission DCP Raptor Pipeline, LLC; Notice of Compliance Filing February 22, 2010. Take notice that on January 28, 2010, DCP Raptor Pipeline, LLC (Raptor) filed its Statement of... Docket Nos. PR09-32-000 and PR09-32-001. Raptor states that it made revisions to include a statement of...

  2. Common File Formats.

    PubMed

    Mills, Lauren

    2014-03-21

    An overview of the many file formats commonly used in bioinformatics and genome sequence analysis is presented, including various data file formats, alignment file formats, and annotation file formats. Example workflows illustrate how some of the different file types are typically used.

  3. Morphological and genetic variation in North Atlantic giant file clams, Acesta spp. (Bivalvia: Limidae), with description of a new cryptic species in the northwest Atlantic.

    PubMed

    Gagnon, Jean-Marc; Kenchington, Ellen; Port, Antony; Anstey, Lynne J; Murillo, Francisco Javier

    2015-08-27

    We analyze the morphological and genetic variability within and between seven species of Acesta and specimens recently collected in the northwest Atlantic using traditional morphological measurements, landmark-based geometric morphometrics, and the cytochrome oxidase subunit I (COI) gene sequences, with particular emphasis on North Atlantic species. Shell morphology and external shell appearance do not allow reliable distinction between the widely recognized northeastern Atlantic A. excavata and other northwest Atlantic species or populations of Acesta, with the exception of A. oophaga. Similarly, shape analysis reveals a wide variability within northeastern Atlantic A. excavata, and significant morphological overlap with A. bullisi from the Gulf of Mexico and A. rathbuni from the southwestern Pacific and South China Sea. Specimens from the northwestern and Mid-Atlantic display shell shapes marginally similar to that of A. excavata. These differences are at least partly related to anterior or posterior shifting of the shell body and to the opposite shifting of the hinge line/dorsal region and upper lunule. These morphological variations, along with the midline-width-ratio, explain much of the variability extracted by principal component analysis. Results from a mitochondrial DNA barcode approach (COI), however, suggest that the northwest Atlantic specimens belong to a new species for which we propose the name Acesta cryptadelphe sp. nov. Differences in larval shell sizes between northeastern and northwestern Atlantic specimens are consistent with this result.

  4. 43 CFR 4.1352 - Who may file; where to file; when to file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file; where to file; when to file... Indian Lands) § 4.1352 Who may file; where to file; when to file. (a) The applicant or operator may file... to file a timely request constitutes a waiver of the opportunity for a hearing before OSM makes...

  5. NOS1 — EDRN Public Portal

    Cancer.gov

    NOS1, or nitric oxide synthase 1 (neuronal), along with inducible nitric oxide synthase (NOS2) and endothelial nitric oxide synthase (NOS3), catalyze the generation of nitric oxide and L-citrulline from L-arginine and molecular oxygen. Nitric oxide is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter. NOS1 also displays antimicrobial and antitumoral activities.

  6. Report filing in histopathology.

    PubMed Central

    Blenkinsopp, W K

    1977-01-01

    An assessment of alternative methods of filing histopathology report forms in alphabetical order showed that orthodox card index filing is satisfactory up to about 100000 reports but, because of the need for long-term retrieval, when the reports filed exceed this number they should be copied on jacketed microfilm and a new card index file begun. PMID:591645

  7. 76 FR 74109 - Self-Regulatory Organizations; NASDAQ OMX BX, Inc.; Notice of Filing and Immediate Effectiveness...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-30

    .... See Securities Exchange Act Release No. 62252 (June 10, 2010), 75 FR 34186 (June 16, 2010) (File Nos...-CBOE- 2010-047), and Securities Exchange Act Release No. 62251 (June 10, 2010), 75 FR 34183 (June 16... Securities Exchange Act Release No. 62884 (September 10, 2010), 75 FR 56618 (September 16, 2010) (File...

  8. The impact of human and mouse differences in NOS2 gene expression on the brain's redox and immune environment.

    PubMed

    Hoos, Michael D; Vitek, Michael P; Ridnour, Lisa A; Wilson, Joan; Jansen, Marilyn; Everhart, Angela; Wink, David A; Colton, Carol A

    2014-11-17

    Mouse models are used in the study of human disease. Despite well-known homologies, the difference in immune response between mice and humans impacts the application of data derived from mice to human disease outcomes. Nitric oxide synthase-2 (NOS2) is a key gene that displays species-specific outcomes via altered regulation of the gene promoter and via post-transcriptional mechanisms in humans that are not found in mice. The resulting levels of NO produced by activation of human NOS2 are different from the levels of NO produced by mouse Nos2. Since both tissue redox environment and immune responsiveness are regulated by the level of NO and its interactions, we investigated the significance of mouse and human differences on brain oxidative stress and on immune activation in HuNOS2tg/mNos2-/- mice that express the entire human NOS2 gene and that lack a functional mNos2 compared to wild type (WT) mice that express normal mNos2. Similarly to human, brain tissue from HuNOS2tg/mNos2-/- mice showed the presence of a NOS2 gene 3'UTR binding site. We also identified miRNA-939, the binding partner for this site, in mouse brain lysates and further demonstrated reduced levels of nitric oxide (NO) typical of the human immune response on injection with lipopolysaccharide (LPS). HuNOS2tg/mNos2-/- brain samples were probed for characteristic differences in redox and immune gene profiles compared to WT mice using gene arrays. Selected genes were also compared against mNos2-/- brain lysates. Reconstitution of the human NOS2 gene significantly altered genes that encode multiple anti-oxidant proteins, oxidases, DNA repair, mitochondrial proteins and redox regulated immune proteins. Expression levels of typical pro-inflammatory, anti-inflammatory and chemokine genes were not significantly different with the exception of increased TNFα and Ccr1 mRNA expression in the HuNOS2tg/mNos2-/- mice compared to WT or mNos2-/- mice. NO is a principle factor in establishing the tissue redox

  9. COMPARISON OF OXYGEN–INDUCED RADICAL INTERMEDIATES IN iNOS OXYGENASE DOMAIN WITH THOSE FROM nNOS and eNOS

    PubMed Central

    Berka, Vladimir; Liu, Wen; Wu, Gang; Tsai, Ah-lim

    2014-01-01

    Inducible nitric-oxide synthase (iNOS) produces the reactive oxygen and nitrogen species (ROS/RNS) involved in bacteria killing and is crucial in the host defense mechanism. However, high level ROS/RNS can also be detrimental to normal cells and thus their production has to be tightly controlled. Availability or deficiency of tetrahydrobiopterin (BH4) cofactor and L-arginine substrate control coupling or uncoupling of NOS catalysis. Fully coupled reaction, with abundant BH4 and L-arginine, produces NO whereas the uncoupled NOS (in the absence of BH4 and/or L-arginine) generates ROS/RNS. In the current work we focus on direct rapid freeze EPR to characterize the structure and kinetics of oxygen-induced radical intermediates produced by ferrous inducible NOS oxygenase domain (iNOSox) in the presence or absence of BH4 and/or L-arginine. Fully reconstituted iNOSox (+BH4, +L-Arg) forms a dimer and yields a typical BH4 radical that indicates coupled reaction. iNOSox (–BH4) remains mainly monomeric and produces exclusively superoxide, that is only marginally affected by the presence of L-arginine. iNOSox (+BH4, −L-Arg) exists as a monomer/dimer mixture and yields both BH4 radical and superoxide. Present study is a natural extension of our previous work on the ferrous endothelial NOSox (eNOSox) [V. Berka, G. Wu, H.C. Yeh, G. Palmer, A.L. Tsai, J. Biol. Chem. 279 ( 2004) 32243–32251] and ferrous neuronal NOSox (nNOSox) [V. Berka, L.H. Wang, A.L. Tsai, Biochemistry 47 (2008) 405–420]. Overall, our data suggests different regulatory roles of L-arginine and BH4 in the production of oxygen-induced radical intermediates in NOS isoforms which nicely serve individual functional role. PMID:25016313

  10. Comparison of oxygen-induced radical intermediates in iNOS oxygenase domain with those from nNOS and eNOS.

    PubMed

    Berka, Vladimír; Liu, Wen; Wu, Gang; Tsai, Ah-Lim

    2014-10-01

    Inducible nitric-oxide synthase (iNOS) produces the reactive oxygen and nitrogen species (ROS/RNS) involved in bacteria killing and is crucial in the host defense mechanism. However, high level ROS/RNS can also be detrimental to normal cells and thus their production has to be tightly controlled. Availability or deficiency of tetrahydrobiopterin (BH4) cofactor and l-arginine substrate controls coupling or uncoupling of NOS catalysis. Fully coupled reaction, with abundant BH4 and l-arginine, produces NO whereas the uncoupled NOS (in the absence of BH4 and/or l-arginine) generates ROS/RNS. In the current work we focus on direct rapid freeze EPR to characterize the structure and kinetics of oxygen-induced radical intermediates produced by ferrous inducible NOS oxygenase domain (iNOSox) in the presence or absence of BH4 and/or l-arginine. Fully reconstituted iNOSox (+BH4, +L-Arg) forms a dimer and yields a typical BH4 radical that indicates coupled reaction. iNOSox (-BH4) remains mainly monomeric and produces exclusively superoxide, that is only marginally affected by the presence of l-arginine. iNOSox (+BH4, -L-Arg) exists as a monomer/dimer mixture and yields both BH4 radical and superoxide. Present study is a natural extension of our previous work on the ferrous endothelial NOSox (eNOSox) [V. Berka, G. Wu, H.C. Yeh, G. Palmer, A.L. Tsai, J. Biol. Chem. 279 (2004) 32243-32251] and ferrous neuronal NOSox (nNOSox) [V. Berka, L.H. Wang, A.L. Tsai, Biochemistry 47 (2008) 405-420]. Overall, our data suggests different regulatory roles of l-arginine and BH4 in the production of oxygen-induced radical intermediates in NOS isoforms which nicely serve individual functional role.

  11. Reversal of SIN-1-induced eNOS dysfunction by the spin trap, DMPO, in bovine aortic endothelial cells via eNOS phosphorylation

    PubMed Central

    Das, Amlan; Gopalakrishnan, Bhavani; Druhan, Lawrence J; Wang, Tse-Yao; De Pascali, Francesco; Rockenbauer, Antal; Racoma, Ira; Varadharaj, Saradhadevi; Zweier, Jay L; Cardounel, Arturo J; Villamena, Frederick A

    2014-01-01

    Background and Purpose Nitric oxide (NO) derived from eNOS is mostly responsible for the maintenance of vascular homeostasis and its decreased bioavailability is characteristic of reactive oxygen species (ROS)-induced endothelial dysfunction (ED). Because 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), a commonly used spin trap, can control intracellular nitroso-redox balance by scavenging ROS and donating NO, it was employed as a cardioprotective agent against ED but the mechanism of its protection is still not clear. This study elucidated the mechanism of protection by DMPO against SIN-1-induced oxidative injury to bovine aortic endothelial cells (BAEC). Experimental Approach BAEC were treated with SIN-1, as a source of peroxynitrite anion (ONOO−), and then incubated with DMPO. Cytotoxicity following SIN-1 alone and cytoprotection by adding DMPO was assessed by MTT assay. Levels of ROS and NO generation from HEK293 cells transfected with wild-type and mutant eNOS cDNAs, tetrahydrobiopterin bioavailability, eNOS activity, eNOS and Akt kinase phosphorylation were measured. Key Results Post-treatment of cells with DMPO attenuated SIN-1-mediated cytotoxicity and ROS generation, restoration of NO levels via increased in eNOS activity and phospho-eNOS levels. Treatment with DMPO alone significantly increased NO levels and induced phosphorylation of eNOS Ser1179 via Akt kinase. Transfection studies with wild-type and mutant human eNOS confirmed the dual role of eNOS as a producer of superoxide anion (O2−) with SIN-1 treatment, and a producer of NO in the presence of DMPO. Conclusion and Implications Post-treatment with DMPO of oxidatively challenged cells reversed eNOS dysfunction and could have pharmacological implications in the treatment of cardiovascular diseases. PMID:24405159

  12. 43 CFR 4.1381 - Who may file; when to file; where to file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file; when to file; where to file... may file; when to file; where to file. (a) Any person who receives a written decision issued by OSM under 30 CFR 773.28 on a challenge to an ownership or control listing or finding may file a request...

  13. The return of the Scarlet Pimpernel: cobalamin in inflammation II — cobalamins can both selectively promote all three nitric oxide synthases (NOS), particularly iNOS and eNOS, and, as needed, selectively inhibit iNOS and nNOS

    PubMed Central

    Wheatley, Carmen

    2007-01-01

    The up-regulation of transcobalamins [hitherto posited as indicating a central need for cobalamin (Cbl) in inflammation], whose expression, like inducible nitric oxide synthase (iNOS), is Sp1- and interferondependent, together with increased intracellular formation of glutathionylcobalamin (GSCbl), adenosylcobalamin (AdoCbl), methylcobalamin (MeCbl), may be essential for the timely promotion and later selective inhibition of iNOS and concordant regulation of endothelial and neuronal NOS (eNOS/nNOS.) Cbl may ensure controlled high output of nitric oxide (NO) and its safe deployment, because: (1) Cbl is ultimately responsible for the synthesis or availability of the NOS substrates and cofactors heme, arginine, BH4 flavin adenine dinucleotide/flavin mononucleotide (FAD/FMN) and NADPH, via the far-reaching effects of the two Cbl coenzymes, methionine synthase (MS) and methylmalonyl CoA mutase (MCoAM) in, or on, the folate, glutathione, tricarboxylic acid (TCA) and urea cycles, oxidative phosphorylation, glycolysis and the pentose phosphate pathway. Deficiency of any of theNOS substrates and cofactors results in ‘uncoupled’ NOS reactions, decreasedNO production and increased or excessive O2−, H2O2, ONOO− and other reactive oxygen species (ROS), reactive nitric oxide species (RNIS) leading to pathology. (2) Cbl is also the overlooked ultimate determinant of positive glutathione status, which favours the formation of more benign NO species, s-nitrosothiols, the predominant form in which NO is safely deployed. Cbl status may consequently act as a ‘back-up disc’ that ensures the active status of antioxidant systems, as well as reversing and modulating the effects of nitrosylation in cell signal transduction.New evidence shows that GSCbl can significantly promote iNOS/ eNOS NO synthesis in the early stages of inflammation, thus lowering high levels of tumour necrosis factor-a that normally result in pathology, while existing evidence shows that in extreme

  14. Nox2-dependent glutathionylation of endothelial NOS leads to uncoupled superoxide production and endothelial barrier dysfunction in acute lung injury.

    PubMed

    Wu, Feng; Szczepaniak, William S; Shiva, Sruti; Liu, Huanbo; Wang, Yinna; Wang, Ling; Wang, Ying; Kelley, Eric E; Chen, Alex F; Gladwin, Mark T; McVerry, Bryan J

    2014-12-15

    Microvascular barrier integrity is dependent on bioavailable nitric oxide (NO) produced locally by endothelial NO synthase (eNOS). Under conditions of limited substrate or cofactor availability or by enzymatic modification, eNOS may become uncoupled, producing superoxide in lieu of NO. This study was designed to investigate how eNOS-dependent superoxide production contributes to endothelial barrier dysfunction in inflammatory lung injury and its regulation. C57BL/6J mice were challenged with intratracheal LPS. Bronchoalveolar lavage fluid was analyzed for protein accumulation, and lung tissue homogenate was assayed for endothelial NOS content and function. Human lung microvascular endothelial cell (HLMVEC) monolayers were exposed to LPS in vitro, and barrier integrity and superoxide production were measured. Biopterin species were quantified, and coimmunoprecipitation (Co-IP) assays were performed to identify protein interactions with eNOS that putatively drive uncoupling. Mice exposed to LPS demonstrated eNOS-dependent increased alveolar permeability without evidence for altered canonical NO signaling. LPS-induced superoxide production and permeability in HLMVEC were inhibited by the NOS inhibitor nitro-l-arginine methyl ester, eNOS-targeted siRNA, the eNOS cofactor tetrahydrobiopterin, and superoxide dismutase. Co-IP indicated that LPS stimulated the association of eNOS with NADPH oxidase 2 (Nox2), which correlated with augmented eNOS S-glutathionylation both in vitro and in vivo. In vitro, Nox2-specific inhibition prevented LPS-induced eNOS modification and increases in both superoxide production and permeability. These data indicate that eNOS uncoupling contributes to superoxide production and barrier dysfunction in the lung microvasculature after exposure to LPS. Furthermore, the results implicate Nox2-mediated eNOS-S-glutathionylation as a mechanism underlying LPS-induced eNOS uncoupling in the lung microvasculature.

  15. Expression profiles of NOS isoforms in gingiva of nNOS knockout mice.

    PubMed

    Ishioka, M; Ishizuka, Y; Shintani, S; Yanagisawa, T; Inoue, T; Sasaki, J; Watanabe, H

    2014-04-01

    Nitric oxide is a gaseous molecule associated with many distinct physiological functions, and is derived from L-arginine catalyzed by nitric oxide synthase (NOS). Nitric oxide synthase has 3 isoforms: nNOS, iNOS and eNOS. Although these NOS isoforms are believed to play an important role in gingival tissue, little information is available on their morphological dynamics. The aim of this study was to investigate the profiles of NOS isoforms in deficiency of nNOS in gingiva of mice. Twelve male (6 normal (C57BL/6) and 6 nNOS knockout) mice were used. All mice were 5-week-old, weighing approximately 20-25 g each. After sacrifice, the jaws of the mice were removed by mechanical means and specimens analyzed by histology, in situ hybridization and immunohistochemistry. Immunohistochemical observation revealed positive staining for iNOS and eNOS, especially in lamina propria. Similar results in the mRNA expression levels were shown by in situ hybridization analysis. It may suggest that iNOS and eNOS compensated nNOS deficiency in the gingiva of nNOS knockout mice.

  16. Liposomal tetrahydrobiopterin preserves eNOS coupling in the post-ischemic heart conferring in vivo cardioprotection.

    PubMed

    Xie, Lin; Talukder, M A Hassan; Sun, Jian; Varadharaj, Saradhadevi; Zweier, Jay L

    2015-09-01

    Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthase (NOS), and reduced BH4 availability leads to endothelial NOS (eNOS) uncoupling and increased reactive oxygen species (ROS) generation. Questions remain regarding the functional state of eNOS and role of BH4 availability in the process of in vivo myocardial ischemia-reperfusion (I/R) injury. Rats were subjected to 60min of in vivo left coronary artery occlusion and varying periods of reperfusion with or without pre-ischemic liposomal BH4 supplementation (1mg/kg, iv). Myocardial infarction was correlated with cardiac BH4 content, eNOS protein level, NOS enzyme activity, and ROS generation. In the vehicle group, 60-min ischemia drastically reduced myocardial BH4 content in the area at risk (AAR) compared to non-ischemic (NI) area and the level remained lower during early reperfusion followed by recovery after 24-h reperfusion. Total eNOS, activated eNOS protein level (eNOS Ser1177 phosphorylation) and NOS activity were also significantly reduced during ischemia and/or early reperfusion, but recovered after 24-h reperfusion. With liposomal BH4 treatment, BH4 levels were identical in the AAR and NI area during ischemia and/or early reperfusion, and were significantly higher than with vehicle. BH4 pre-treatment preserved eNOS Ser1177 phosphorylation and NOS activity in the AAR, and significantly reduced myocardial ROS generation and infarction compared to vehicle. These findings provide direct evidence that in vivo I/R induces eNOS dysfunction secondary to BH4 depletion, and that pre-ischemic liposomal BH4 administration preserves eNOS function conferring cardioprotection with reduced oxidative stress. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Role of muscular eNOS in skeletal arteries: Endothelium-independent hypoxic vasoconstriction of the femoral artery is impaired in eNOS-deficient mice.

    PubMed

    Kim, Hae Jin; Yoo, Hae Young; Lin, Hai Yue; Oh, Goo Taeg; Zhang, Yin Hua; Kim, Sung Joon

    2016-09-01

    We previously reported that hypoxia augments α-adrenergic contraction (hypoxic vasoconstriction, HVC) of skeletal arteries in rats. The underlying mechanism may involve hypoxic inhibition of endothelial nitric oxide synthase (eNOS) expressed in skeletal arterial myocytes (16). To further explore the novel role of muscular eNOS in the skeletal artery, we compared HVC in femoral arteries (FAs) from eNOS knockout (KO) mice with that from wild-type (WT) and heterozygous (HZ) mice. Immunohistochemical assays revealed that, in addition to endothelia, eNOS is also expressed in the medial layer of FAs, albeit at a much lower level. However, the medial eNOS signal was not evident in HZ FAs, despite strong expression in the endothelium; similar observations were made in WT carotid arteries (CAs). The amplitude of contraction induced by 1 μM phenylephrine (PhE) was greater in HZ than in WT FAs. Hypoxia (3% Po2) significantly augmented PhE-induced contraction in WT FAs but not in HZ or KO FAs. No HVC was observed in PhE-pretreated WT CAs. The NOS inhibitor nitro-l-arginine methyl ester (0.1 mM) also augmented PhE contraction in endothelium-denuded WT FAs but not in WT CAs. Inhibitors specific to neuronal NOS and inducible NOS did not augment PhE-induced contraction of WT FAs. NADPH oxidase 4 (NOX4) inhibitor (GKT137831, 5 μM), but not NOX2 inhibitor (apocynin, 100 μM), suppressed HVC. Consistent with the role of reactive oxygen species (ROS), HVC was also inhibited by pretreatment with tiron or polyethylene glycol-catalase. Taken together, these data suggest that the eNOS expressed in smooth muscle cells in FAs attenuates α-adrenergic vasoconstriction; this suppression is alleviated under hypoxia, which potentiates vasoconstriction in a NOX4/ROS-dependent mechanism. Copyright © 2016 the American Physiological Society.

  18. Vascular and Perivascular NO Release and Transport: Biochemical Pathways of NOS1 and NOS3

    PubMed Central

    Chen, Kejing; Popel, Aleksander S.

    2007-01-01

    Nitric oxide (NO) derived from nitric oxide synthase (NOS) is an important paracrine effector that maintains vascular tone. The release of NO mediated by NOS isozymes under various O2 conditions critically determines the NO bioavailability in tissues. Because of experimental difficulties, there has been no direct information on how enzymatic NO production and distribution change around arterioles under various oxygen conditions. In this study, we used computational models based on the analysis of biochemical pathways of enzymatic NO synthesis and the availability of NOS isozymes to quantify the NO production by neuronal NOS (NOS1) and endothelial NOS (NOS3). We compared the catalytic activities of NOS1 and NOS3 and their sensitivities to the concentration of substrate O2. Based on the NO release rates predicted from kinetic models, the geometric distribution of NO sources and mass balance analysis, we predicted the NO concentration profiles around an arteriole under various O2 conditions. The results indicated that NOS1-catalyzed NO production was significantly more sensitive to ambient O2 concentration than that catalyzed by NOS3. Also, the high sensitivity of NOS1 catalytic activity to O2 was associated with significantly reduced NO production and therefore NO concentrations, upon hypoxia. Moreover, the major source determining the distribution of NO was NOS1, which was abundantly expressed in the nerve fibers and mast cells close to arterioles, rather than NOS3, which was expressed in the endothelium. Finally, the perivascular NO concentration predicted by the models under conditions of normoxia was paradoxically at least an order of magnitude lower than a number of experimental measurements, suggesting a higher abundance of NOS1 or NOS3 and/or the existence of other enzymatic or non-enzymatic sources of NO in the microvasculature. PMID:17320763

  19. Text File Display Program

    NASA Technical Reports Server (NTRS)

    Vavrus, J. L.

    1986-01-01

    LOOK program permits user to examine text file in pseudorandom access manner. Program provides user with way of rapidly examining contents of ASCII text file. LOOK opens text file for input only and accesses it in blockwise fashion. Handles text formatting and displays text lines on screen. User moves forward or backward in file by any number of lines or blocks. Provides ability to "scroll" text at various speeds in forward or backward directions.

  20. Standard interface file handbook

    SciTech Connect

    Shapiro, A.; Huria, H.C. )

    1992-10-01

    This handbook documents many of the standard interface file formats that have been adopted by the US Department of Energy to facilitate communications between and portability of, various large reactor physics and radiation transport software packages. The emphasis is on those files needed for use of the VENTURE/PC diffusion-depletion code system. File structures, contents and some practical advice on use of the various files are provided.

  1. Text File Comparator

    NASA Technical Reports Server (NTRS)

    Kotler, R. S.

    1983-01-01

    File Comparator program IFCOMP, is text file comparator for IBM OS/VScompatable systems. IFCOMP accepts as input two text files and produces listing of differences in pseudo-update form. IFCOMP is very useful in monitoring changes made to software at the source code level.

  2. Text File Comparator

    NASA Technical Reports Server (NTRS)

    Kotler, R. S.

    1983-01-01

    File Comparator program IFCOMP, is text file comparator for IBM OS/VScompatable systems. IFCOMP accepts as input two text files and produces listing of differences in pseudo-update form. IFCOMP is very useful in monitoring changes made to software at the source code level.

  3. Bioinformatic comparisons and tissue expression of the neuronal nitric oxide synthase (nNOS) gene from the red drum (Sciaenops ocellatus).

    PubMed

    Zhou, Libin; Bai, Ru; Tian, Jianxiao; Liu, Xiaochun; Lu, Danqi; Zhu, Pei; Liu, Yun; Zeng, Lujiao; Luo, Wenna; Zhang, Yong; Wang, Anli

    2009-10-01

    The full length cDNA sequence for neuronal nitric oxide synthase (nNOS) gene from red drum (Sciaenops ocellatus) has been cloned, subjected to bioinformatic analysis, and examined for expression in different tissues. Red drum nNOS showed high identity to nNOS of mammals and other fish species. Notably, a unique 7-aa insertion was found in the important catalytic sites of the NO synthase domain, possibly affecting the function of red drum nNOS. Furthermore, this nNOS was expressed not only in brain but also in most of the internal organs including liver, intestine, spleen, head kidney and thymus.

  4. Liposomal Tetrahydrobiopterin Preserves eNOS Coupling in the Post-ischemic Heart Conferring in vivo Cardioprotection

    PubMed Central

    Xie, Lin; Talukder, M A Hassan; Sun, Jian; Varadharaj, Saradhadevi; Zweier, Jay L.

    2015-01-01

    Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthase (NOS), and reduced BH4 availability leads to endothelial NOS (eNOS) uncoupling and increased reactive oxygen species (ROS) generation. Questions remain regarding the functional state of eNOS and role of BH4 availability in the process of in vivo myocardial ischemia-reperfusion (I/R) injury. Rats were subjected to 60-minutes of in vivo left coronary artery occlusion and varying periods of reperfusion with or without pre-ischemic liposomal BH4 supplementation (1 mg/kg, iv). Myocardial infarction was correlated with cardiac BH4 content, eNOS protein level, NOS enzyme activity, and ROS generation. In the vehicle group, 60-min ischemia drastically reduced myocardial BH4 content in the area at risk (AAR) compared to non-ischemic (NI) area and the level remained lower during early reperfusion followed by recovery after 24-hr reperfusion. Activated eNOS protein level (eNOS Ser1177 phosphorylation) and NOS activity were also significantly reduced during ischemia and/or early reperfusion, but recovered after 24-hr reperfusion. With liposomal BH4 treatment, BH4 levels were identical in the AAR and NI area during ischemia and/or early reperfusion, and were significantly higher than with vehicle. BH4 pre-treatment preserved eNOS Ser1177 phosphorylation and NOS activity in the AAR, and significantly reduced myocardial ROS generation and infarction compared to vehicle. These findings provide direct evidence that in vivo I/R induces eNOS dysfunction secondary to BH4 depletion, and that pre-ischemic liposomal BH4 administration preserves eNOS function conferring cardioprotection with reduced oxidative stress. PMID:26116866

  5. 77 FR 39999 - Marine Mammals; File No. 17278

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-06

    ...'' from the Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 17278 from the list of available applications. These...

  6. 77 FR 48967 - Marine Mammals; File No. 17350

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-15

    ... marine mammals of the following species: bearded seal (Erignathus ] barbatus), ringed seal (Phoca hispida... National Oceanic and Atmospheric Administration RIN 0648-XC067 Marine Mammals; File No. 17350 AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA),...

  7. 43 CFR 4.1371 - Who may file, where to file, when to file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file, where to file, when to file... Permits § 4.1371 Who may file, where to file, when to file. (a) A permittee that is served with a notice... under 30 CFR 773.23 may file a request for review with the Hearings Division, Office of Hearings...

  8. Nitric oxide synthase enzymes in the airways of mice exposed to ovalbumin: NOS2 expression is NOS3 dependent.

    PubMed

    Bratt, Jennifer M; Williams, Keisha; Rabowsky, Michelle F; Last, Michael S; Franzi, Lisa M; Last, Jerold A; Kenyon, Nicholas J

    2010-01-01

    The function of the airway nitric oxide synthase (NOS) isoforms and the lung cell types responsible for its production are not fully understood. We hypothesized that NO homeostasis in the airway is important to control inflammation, which requires upregulation, of NOS2 protein expression by an NOS3-dependent mechanism. Mice from a C57BL/6 wild-type, NOS1(-/-), NOS2(-/-), and NOS3(-/-) genotypes were used. All mice strains were systemically sensitized and exposed to filtered air or ovalbumin (OVA) aerosol for two weeks to create a subchronic model of allergen-induced airway inflammation. We measured lung function, lung lavage inflammatory and airway epithelial goblet cell count, exhaled NO, nitrate and nitrite concentration, and airway NOS1, NOS2, and NOS3 protein content. Deletion of NOS1 or NOS3 increases NOS2 protein present in the airway epithelium and smooth muscle of air-exposed animals. Exposure to allergen significantly reduced the expression of NOS2 protein in the airway epithelium and smooth muscle of the NOS3(-/-) strain only. This reduction in NOS2 expression was not due to the replacement of epithelial cells with goblet cells as remaining epithelial cells did not express NOS2. NOS1(-/-) animals had significantly reduced goblet cell metaplasia compared to C57Bl/6 wt, NOS2(-/-), and NOS3(-/-) allergen-exposed mice. The airway epithelial and smooth muscle cells maintain a stable airway NO concentration under noninflammatory conditions. This "homeostatic" mechanism is unable to distinguish between NOS derived from the different constitutive NOS isoforms. NOS3 is essential for the expression of NOS2 under inflammatory conditions, while NOS1 expression contributes to allergen-induced goblet cell metaplasia.

  9. Nitric Oxide Synthase Enzymes in the Airways of Mice Exposed to Ovalbumin: NOS2 Expression Is NOS3 Dependent

    PubMed Central

    Bratt, Jennifer M.; Williams, Keisha; Rabowsky, Michelle F.; Last, Michael S.; Franzi, Lisa M.; Last, Jerold A.; Kenyon, Nicholas J.

    2010-01-01

    Objectives and Design. The function of the airway nitric oxide synthase (NOS) isoforms and the lung cell types responsible for its production are not fully understood. We hypothesized that NO homeostasis in the airway is important to control inflammation, which requires upregulation, of NOS2 protein expression by an NOS3-dependent mechanism. Materials or Subjects. Mice from a C57BL/6 wild-type, NOS1−/−, NOS2−/−, and NOS3−/− genotypes were used. All mice strains were systemically sensitized and exposed to filtered air or ovalbumin (OVA) aerosol for two weeks to create a subchronic model of allergen-induced airway inflammation. Methods. We measured lung function, lung lavage inflammatory and airway epithelial goblet cell count, exhaled NO, nitrate and nitrite concentration, and airway NOS1, NOS2, and NOS3 protein content. Results. Deletion of NOS1 or NOS3 increases NOS2 protein present in the airway epithelium and smooth muscle of air-exposed animals. Exposure to allergen significantly reduced the expression of NOS2 protein in the airway epithelium and smooth muscle of the NOS3−/− strain only. This reduction in NOS2 expression was not due to the replacement of epithelial cells with goblet cells as remaining epithelial cells did not express NOS2. NOS1−/− animals had significantly reduced goblet cell metaplasia compared to C57Bl/6 wt, NOS2−/−, and NOS3−/− allergen-exposed mice. Conclusion. The airway epithelial and smooth muscle cells maintain a stable airway NO concentration under noninflammatory conditions. This “homeostatic” mechanism is unable to distinguish between NOS derived from the different constitutive NOS isoforms. NOS3 is essential for the expression of NOS2 under inflammatory conditions, while NOS1 expression contributes to allergen-induced goblet cell metaplasia. PMID:20953358

  10. Sensitivity Data File Formats

    SciTech Connect

    Rearden, Bradley T.

    2016-04-01

    The format of the TSUNAMI-A sensitivity data file produced by SAMS for cases with deterministic transport solutions is given in Table 6.3.A.1. The occurrence of each entry in the data file is followed by an identification of the data contained on each line of the file and the FORTRAN edit descriptor denoting the format of each line. A brief description of each line is also presented. A sample of the TSUNAMI-A data file for the Flattop-25 sample problem is provided in Figure 6.3.A.1. Here, only two profiles out of the 130 computed are shown.

  11. 21 CFR 516.147 - Refuse to file a request for addition to the index.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 6 2014-04-01 2014-04-01 false Refuse to file a request for addition to the index. 516.147 Section 516.147 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.147 Refuse to file...

  12. 21 CFR 516.171 - Confidentiality of data and information in an index file.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... index file. 516.171 Section 516.171 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... USE AND MINOR SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.171 Confidentiality of data and information in an index file. (a) For purposes of this section,...

  13. 21 CFR 516.147 - Refuse to file a request for addition to the index.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Refuse to file a request for addition to the index. 516.147 Section 516.147 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.147 Refuse to file...

  14. 21 CFR 516.147 - Refuse to file a request for addition to the index.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 6 2011-04-01 2011-04-01 false Refuse to file a request for addition to the index. 516.147 Section 516.147 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.147 Refuse to file...

  15. 21 CFR 516.171 - Confidentiality of data and information in an index file.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... index file. 516.171 Section 516.171 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... USE AND MINOR SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.171 Confidentiality of data and information in an index file. (a) For purposes of this section,...

  16. 21 CFR 516.147 - Refuse to file a request for addition to the index.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 6 2013-04-01 2013-04-01 false Refuse to file a request for addition to the index. 516.147 Section 516.147 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.147 Refuse to file...

  17. 21 CFR 516.147 - Refuse to file a request for addition to the index.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 6 2012-04-01 2012-04-01 false Refuse to file a request for addition to the index. 516.147 Section 516.147 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.147 Refuse to file...

  18. 21 CFR 516.171 - Confidentiality of data and information in an index file.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... index file. 516.171 Section 516.171 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... USE AND MINOR SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.171 Confidentiality of data and information in an index file. (a) For purposes of this section,...

  19. 75 FR 30876 - Self-Regulatory Organizations; Municipal Securities Rulemaking Board; Notice of Filing of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Self-Regulatory Organizations; Municipal Securities Rulemaking Board; Notice of Filing of Amendment No. 2 and Order Granting Accelerated Approval of Proposed Rule Change, as Modified by Amendment Nos. 1 and 2 Thereto, Relating to Additional...

  20. Register file soft error recovery

    DOEpatents

    Fleischer, Bruce M.; Fox, Thomas W.; Wait, Charles D.; Muff, Adam J.; Watson, III, Alfred T.

    2013-10-15

    Register file soft error recovery including a system that includes a first register file and a second register file that mirrors the first register file. The system also includes an arithmetic pipeline for receiving data read from the first register file, and error detection circuitry to detect whether the data read from the first register file includes corrupted data. The system further includes error recovery circuitry to insert an error recovery instruction into the arithmetic pipeline in response to detecting the corrupted data. The inserted error recovery instruction replaces the corrupted data in the first register file with a copy of the data from the second register file.

  1. Multifaceted NOS Instruction: Contextualizing Nature of Science with Documentary Films

    ERIC Educational Resources Information Center

    Bloom, Mark; Binns, Ian C.; Koehler, Catherine

    2015-01-01

    This research focuses on inservice science teachers' conceptions of nature of science (NOS) before and after a two-week intensive summer professional development (PD). The PD combined traditional explicit NOS instruction, numerous interactive interventions that highlighted NOS aspects, along with documentary films that portrayed NOS in context of…

  2. Viewing Files | Smokefree 60+

    Cancer.gov

    In addition to standard HTML webpages, our website contains files in other formats. You may need additional software or browser plug-ins to view some of these files. The following list shows each format along with links to the corresponding freely available plug-ins or viewers. Documents  Adobe Acrobat Reader (.pdf)

  3. Hopper File Management Tool

    SciTech Connect

    Long, J W; O'Neill, N J; Smith, N G; Springmeyer, R R; Remmele, S; Richards, D A; Southon, J

    2004-11-15

    Hopper is a powerful interactive tool that allows users to transfer and manipulate files and directories by means of a graphical user interface. Users can connect to and manage resources using the major file transfer protocols. Implemented in Java, Hopper can be run almost anywhere: from an individual's desktop machine to large production machines. In a high-performance computing environment, managing files can become a difficult and time-consuming task that distracts from scientific work. Users must deal with multiple file transfer protocols, transferring enormous amounts of files between computer platforms, repeated authentication, organizing massive amounts of data, and other detailed but necessary tasks. This is often accomplished with a set of several different tools, each with its own interface and idiosyncrasies. Our goal is to develop tools for a more automated approach to file management that substantially improves users' ability to transfer, organize, search, and operate on collections of files. This paper describes the Hopper tool for advanced file management, including the software architecture, the functionality, and the user interface.

  4. NASA Uniform Files Index

    NASA Technical Reports Server (NTRS)

    1987-01-01

    This handbook is a guide for the use of all personnel engaged in handling NASA files. It is issued in accordance with the regulations of the National Archives and Records Administration, in the Code of Federal Regulations Title 36, Part 1224, Files Management; and the Federal Information Resources Management Regulation, Subpart 201-45.108, Files Management. It is intended to provide a standardized classification and filing scheme to achieve maximum uniformity and ease in maintaining and using agency records. It is a framework for consistent organization of information in an arrangement that will be useful to current and future researchers. The NASA Uniform Files Index coding structure is composed of the subject classification table used for NASA management directives and the subject groups in the NASA scientific and technical information system. It is designed to correlate files throughout NASA and it is anticipated that it may be useful with automated filing systems. It is expected that in the conversion of current files to this arrangement it will be necessary to add tertiary subjects and make further subdivisions under the existing categories. Established primary and secondary subject categories may not be changed arbitrarily. Proposals for additional subject categories of NASA-wide applicability, and suggestions for improvement in this handbook, should be addressed to the Records Program Manager at the pertinent installation who will forward it to the NASA Records Management Office, Code NTR, for approval. This handbook is issued in loose-leaf form and will be revised by page changes.

  5. JENDL Dosimetry File 99.

    SciTech Connect

    KEIICHI,; SHIBATA,

    2001-01-22

    Version 00 JENDL/D-99 contains information for 47 nuclides and 67 reactions in the SAND-II group structure (although it was observed by RSICC that not all of the processed files are in the SAND-II group structure) and as 0K preprocessed pointwise files.

  6. NADPH Oxidase 4 Promotes Endothelial Angiogenesis Through eNOS Activation

    PubMed Central

    Craige, Siobhan M.; Kai, Chen; Pei, Yongmei; Chunying, Li; Xiaoyun, Huang; Christine, Chen; Shibata, Rei; Sato, Kaori; Walsh, Kenneth; Keaney, John F.

    2013-01-01

    Background Reactive Oxygen Species (ROS) serve signaling functions in the vasculature, and hypoxia has been associated with increased ROS production. NADPH oxidase 4 (Nox4) is an ROS-producing enzyme that is highly expressed in the endothelium, yet its specific role is unknown. We sought to determine the role of Nox4 in the endothelial response to hypoxia. Methods and Results Hypoxia induced Nox4 expression both in vitro and in vivo and overexpression of Nox4 was sufficient to promote endothelial proliferation, migration, and tube formation. To determine the in vivo relevance of our observations, we generated transgenic mice with endothelial-specific Nox4 overexpression using the VE-cadherin promoter (VECad-Nox4 mice). In vivo, the VECad-Nox4 mice had accelerated recovery from hind limb ischemia and enhanced aortic capillary sprouting. Because endothelial nitric oxide synthase (eNOS) is involved in endothelial angiogenic responses and eNOS is activated by ROS, we probed the effect of Nox4 on eNOS. In cultured ECs overexpressing Nox4 we observed a significant increase in eNOS protein expression and activity. To causally address the link between eNOS and Nox4 we crossed our transgenic Nox4 mice with eNOS-/- mice. Aorta from these mice did not demonstrate enhanced aortic sprouting and VECad-Nox4 mice on the eNOS-/- background did not demonstrate enhanced recovery from hind limb ischemia. Conclusions Collectively, we demonstrate that augmented endothelial Nox4 expression promotes angiogenesis and recovery from hypoxia in an eNOS-dependent manner. PMID:21788590

  7. Carbon monoxide increases inducible NOS expression that mediates CO-induced myocardial damage during ischemia-reperfusion.

    PubMed

    Meyer, Grégory; André, Lucas; Kleindienst, Adrien; Singh, François; Tanguy, Stéphane; Richard, Sylvain; Obert, Philippe; Boucher, François; Jover, Bernard; Cazorla, Olivier; Reboul, Cyril

    2015-04-01

    We investigated the role of inducible nitric oxide (NO) synthase (iNOS) on ischemic myocardial damage in rats exposed to daily low nontoxic levels of carbon monoxide (CO). CO is a ubiquitous environmental pollutant that impacts on mortality and morbidity from cardiovascular diseases. We have previously shown that CO exposure aggravates myocardial ischemia-reperfusion (I/R) injury partly because of increased oxidative stress. Nevertheless, cellular mechanisms underlying cardiac CO toxicity remain hypothetical. Wistar rats were exposed to simulated urban CO pollution for 4 wk. First, the effects of CO exposure on NO production and NO synthase (NOS) expression were evaluated. Myocardial I/R was performed on isolated perfused hearts in the presence or absence of S-methyl-isothiourea (1 μM), a NOS inhibitor highly specific for iNOS. Finally, Ca(2+) handling was evaluated in isolated myocytes before and after an anoxia-reoxygenation performed with or without S-methyl-isothiourea or N-acetylcystein (20 μM), a nonspecific antioxidant. Our main results revealed that 1) CO exposure altered the pattern of NOS expression, which is characterized by increased neuronal NOS and iNOS expression; 2) cardiac NO production increased in CO rats because of its overexpression of iNOS; and 3) the use of a specific inhibitor of iNOS reduced myocardial hypersensitivity to I/R (infarct size, 29 vs. 51% of risk zone) in CO rat hearts. These last results are explained by the deleterious effects of NO and reactive oxygen species overproduction by iNOS on diastolic Ca(2+) overload and myofilaments Ca(2+) sensitivity. In conclusion, this study highlights the involvement of iNOS overexpression in the pathogenesis of simulated urban CO air pollution exposure.

  8. Registered File Support for Critical Operations Files at SIRTF

    NASA Astrophysics Data System (ADS)

    Turek, G.; Handley, T.; Jacobson, J.; Rector, J.

    The SIRTF Science Center's (SSC) Science Operations System (SOS) has to contend with nearly one hundred critical operations files via comprehensive file management services. The management is accomplished via the registered file system (otherwise known as TFS) which manages these files in a registered file repository composed of a virtual file system accessible via a TFS server and a file registration database. The TFS server provides controlled, reliable, and secure file transfer and storage by registering all file transactions and meta-data in the file registration database. An API is provided for application programs to communicate with TFS servers and the repository. A command line client implementing this API has been developed as a client tool. This paper describes the architecture, current implementation, and more importantly, the evolution of these services based on evolving community use cases and emerging information system technology.

  9. Construction of file database management

    SciTech Connect

    MERRILL,KYLE J.

    2000-03-01

    This work created a database for tracking data analysis files from multiple lab techniques and equipment stored on a central file server. Experimental details appropriate for each file type are pulled from the file header and stored in a searchable database. The database also stores specific location and self-directory structure for each data file. Queries can be run on the database according to file type, sample type or other experimental parameters. The database was constructed in Microsoft Access and Visual Basic was used for extraction of information from the file header.

  10. Catalytic Promiscuity of the Radical S-adenosyl-L-methionine Enzyme NosL

    PubMed Central

    Ding, Wei; Ji, Xinjian; Li, Yongzhen; Zhang, Qi

    2016-01-01

    Catalytic promiscuity plays a key role in enzyme evolution and the acquisition of novel biological functions. Because of the high reactivity of radical species, in our view enzymes involving radical-mediated mechanisms could intrinsically be more prone to catalytic promiscuity. This mini-review summarizes the recent advances in the study of NosL, a radical S-adenosyl-L-methionine (SAM)-dependent L-tryptophan (L-Trp) lyase. We demonstrate here the interesting chemistry and remarkable catalytic promiscuity of NosL, and attempt to highlight the high evolvability of radical SAM enzymes and the potential to engineer these enzymes for novel and improved activities. PMID:27446906

  11. Selective File Dumper

    NASA Astrophysics Data System (ADS)

    Bassetti, Nanni; Frati, Denis

    During a computer forensics investigation we faced a problem how to get all the interesting files we need fast. We work, mainly, using the Open Source software products and Linux OS, and we consider the Sleuthkit and the Foremost two very useful tools, but for reaching our target they were too complicated and time consuming to use. For this reason we developed the Selective File Dumper, a Linux Bash script which makes it possible to extract all the referenced, deleted and unallocated files and finally to perform a keyword search, in a simple way.

  12. 75 FR 30017 - Electronic Tariff Filings; Notice of Posting Regarding Filing Procedures for Electronically Filed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-28

    ..., and for a transition period through September 30, 2010, all tariffs, rate schedules, and... tariff filings related to periods earlier than the baseline filing. Once the Office of the Secretary... required). Electronic Tariff Filings for Periods Earlier Than the Baseline Filing. The electronic tariff...

  13. MAWST file generator (MFG)

    SciTech Connect

    Henriksen, P.W.; Hurdle, S.; Hafer, J.F.

    1993-12-01

    The software program MAWST was developed as a tool to deal with common materials accounting problems. The key to successful usage of this program is in the generation of input files for measurement values, measurement errors, and measurement methods. The program MFG was developed as an aid to creating input files for MAWST. MFG contains three commonly used measurements -- nondestructive assay, (G-T)*C, and V*C -- and a GENERIC measurement as models for data entry for the measurement value file. Sufficient data is collected from the user to produce the measurement error and measurement method files. This report is written as a tutorial presenting and explaining all the options available in MFG by giving examples of execution and the resulting screens that MFG produces.

  14. Converting CSV Files to RKSML Files

    NASA Technical Reports Server (NTRS)

    Trebi-Ollennu, Ashitey; Liebersbach, Robert

    2009-01-01

    A computer program converts, into a format suitable for processing on Earth, files of downlinked telemetric data pertaining to the operation of the Instrument Deployment Device (IDD), which is a robot arm on either of the Mars Explorer Rovers (MERs). The raw downlinked data files are in comma-separated- value (CSV) format. The present program converts the files into Rover Kinematics State Markup Language (RKSML), which is an Extensible Markup Language (XML) format that facilitates representation of operations of the IDD and enables analysis of the operations by means of the Rover Sequencing Validation Program (RSVP), which is used to build sequences of commanded operations for the MERs. After conversion by means of the present program, the downlinked data can be processed by RSVP, enabling the MER downlink operations team to play back the actual IDD activity represented by the telemetric data against the planned IDD activity. Thus, the present program enhances the diagnosis of anomalies that manifest themselves as differences between actual and planned IDD activities.

  15. The SPRY domain–containing SOCS box protein SPSB2 targets iNOS for proteasomal degradation

    PubMed Central

    Kuang, Zhihe; Lewis, Rowena S.; Curtis, Joan M.; Zhan, Yifan; Saunders, Bernadette M.; Babon, Jeffrey J.; Kolesnik, Tatiana B.; Low, Andrew; Masters, Seth L.; Willson, Tracy A.; Kedzierski, Lukasz; Yao, Shenggen; Handman, Emanuela

    2010-01-01

    Inducible nitric oxide (NO) synthase (iNOS; NOS2) produces NO and related reactive nitrogen species, which are critical effectors of the innate host response and are required for the intracellular killing of pathogens such as Mycobacterium tuberculosis and Leishmania major. We have identified SPRY domain–containing SOCS (suppressor of cytokine signaling) box protein 2 (SPSB2) as a novel negative regulator that recruits an E3 ubiquitin ligase complex to polyubiquitinate iNOS, resulting in its proteasomal degradation. SPSB2 interacts with the N-terminal region of iNOS via a binding interface on SPSB2 that has been mapped by nuclear magnetic resonance spectroscopy and mutational analyses. SPSB2-deficient macrophages showed prolonged iNOS expression, resulting in a corresponding increase in NO production and enhanced killing of L. major parasites. These results lay the foundation for the development of small molecule inhibitors that could disrupt the SPSB–iNOS interaction and thus prolong the intracellular lifetime of iNOS, which may be beneficial in chronic and persistent infections. PMID:20603330

  16. 76 FR 54460 - Combined Notice of Filings; Filings Instituting Proceedings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-01

    ... Numbers: RP11-2414-000. Applicants: Pine Needle LNG Company, LLC. Description: Pine Needle LNG Company, LLC submits tariff filing per 154.402: Pine Needle LNG Company 2011 ACA Filing to be effective 10/1...

  17. DSSTOX MASTER STRUCTURE-INDEX FILE: SDF FILE AND DOCUMENTATION

    EPA Science Inventory

    The DSSTox Master Structure-Index File serves to consolidate, manage, and ensure quality and uniformity of the chemical and substance information spanning all DSSTox Structure Data Files, including those in development but not yet published separately on this website.

  18. DSSTOX MASTER STRUCTURE-INDEX FILE: SDF FILE AND DOCUMENTATION

    EPA Science Inventory

    The DSSTox Master Structure-Index File serves to consolidate, manage, and ensure quality and uniformity of the chemical and substance information spanning all DSSTox Structure Data Files, including those in development but not yet published separately on this website.

  19. 15 CFR Supplement Nos. 3-4 to Part... - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Nos. Supplement Nos. 3-4 to Part 742 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF... CONTROLS Supplement Nos. 3-4 to Part 742 ...

  20. 15 CFR Supplement Nos. 3-4 to Part... - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Nos. Supplement Nos. 3-4 to Part 742 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF... CONTROLS Supplement Nos. 3-4 to Part 742 ...

  1. 15 CFR Supplement Nos. 3-4 to Part... - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Nos. Supplement Nos. 3-4 to Part 742 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF... CONTROLS Supplement Nos. 3-4 to Part 742 ...

  2. 15 CFR Supplement Nos. 3-4 to Part... - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Nos. Supplement Nos. 3-4 to Part 742 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF... CONTROLS Supplement Nos. 3-4 to Part 742 ...

  3. 15 CFR Supplement Nos. 3-4 to Part... - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Nos. Supplement Nos. 3-4 to Part 742 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF... CONTROLS Supplement Nos. 3-4 to Part 742 ...

  4. DSSTOX MASTER STRUCTURE-INDEX FILE: SDF FILE AND ...

    EPA Pesticide Factsheets

    The DSSTox Master Structure-Index File serves to consolidate, manage, and ensure quality and uniformity of the chemical and substance information spanning all DSSTox Structure Data Files, including those in development but not yet published separately on this website. The DSSTox Master Structure-Index File serves to consolidate, manage, and ensure quality and uniformity of the chemical and substance information spanning all DSSTox Structure Data Files, including those in development but not yet published separately on this website.

  5. 76 FR 348 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-04

    ... Transmission, LLC submits tariff filing per 154.204: AGT Modifications for Bobcat's Link Implementation Filing... Transmission, L.L.C. submits tariff filing per 154.204: Modifications for Bobcat's LINK Implementation Filing... Transmission, LP submits tariff filing per 154.204: TETLP Filing for Bobcat's Link Implementation to...

  6. XML Files: MedlinePlus

    MedlinePlus

    ... Spanish topic groups. Files generated on October 11, 2017 MedlinePlus Health Topic XML (26957 K) ( DTD , 5 ... DTD , 3 K) Files generated on October 10, 2017 MedlinePlus Health Topic XML (26942 K) ( DTD , 5 ...

  7. Pulmonary hypertension and vascular oxidative damage in cigarette smoke exposed eNOS(-/-) mice and human smokers.

    PubMed

    Wright, J L; Zhou, S; Churg, A

    2012-09-01

    Cigarette smoke is known to be associated with pulmonary hypertension in humans and in animal models. Although the etiology of pulmonary hypertension in smokers is not understood, recent work has suggested a role for inducible nitric oxide synthase (iNOS) in inducing oxidative stress. To further evaluate this question, we assessed eNOS-/- mice exposed to air or cigarette smoke for the presence of pulmonary hypertension and examined vascular remodeling and expression of nitrotyrosine, a marker of reactive nitrogen species-induced oxidative damage, using immunohistochemistry. To ascertain whether oxidants may play a role in humans, we also examined lung tissue from nonsmokers, and patients with chronic obstructive pulmonary disease (COPD) with and without pulmonary hypertension. We found that eNOS(-/-) mice developed increased pulmonary arterial pressure after six months cigarette smoke exposure, and this was associated with vascular remodeling and increased vascular nitrotyrosine staining. iNOS gene expression was decreased in the pulmonary arteries of the smoke exposed animals, and no protein was detectable by immunohistochemistry. In humans, vascular nitrotyrosine staining intensity was increased in smokers with COPD compared to nonsmokers, and further increased in smokers with combined COPD and pulmonary hypertension. We conclude that cigarette smoke-induced pulmonary hypertension is associated with evidence of oxidative vascular damage by reactive nitrogen species, but that iNOS does not appear to be the major contributor to such damage. Most likely the source of reactive nitrogen species is the cigarette smoke itself.

  8. Morphological and physiological study of the cardiac NOS/NO system in the Antarctic (Hb-/Mb-) icefish Chaenocephalus aceratus and in the red-blooded Trematomus bernacchii.

    PubMed

    Garofalo, Filippo; Amelio, Daniela; Cerra, Maria C; Tota, Bruno; Sidell, Bruce D; Pellegrino, Daniela

    2009-03-01

    The nitric oxide synthase (NOS)/nitric oxide (NO) system integrates cellular biochemical machinery and energetics. In heart microenvironment, dynamic NO behaviour depends upon the presence of superoxide anions, haemoglobin (Hb), and myoglobin (Mb), being hemoproteins are major players disarming NO bioactivity. The Antarctic icefish, which lack Hb and, in some species, also cardiac Mb, represent a unique model for exploring Hb and Mb impact on NOS/NO function. We report in the (Hb(-)/Mb(-)) icefish Chaenocephalus aceratus the presence of cardiac NOSs activity (NADPH-diaphorase) and endothelial NOS (eNOS)/inducible NOS (iNOS) zonal immuno-localization in the myocardium. eNOS is localized on endocardium and, to a lesser extent, in myocardiocytes, while iNOS is localized exclusively in myocardiocytes. Confronting eNOS and iNOS expression in Trematomus bernacchii (Hb(+)/Mb(+)), C. hamatus (Hb(-)/Mb(+)) and C. aceratus (Hb(-)/Mb(-)) is evident a lower expression in the Mb-less icefish. NO signaling was analyzed using isolated working heart preparations. In T. bernacchii, L-arginine and exogenous (SIN-1) NO donor dose-dependently decreased stroke volume, indicating decreased inotropism. L-arginine-induced inotropism was NOSs-dependent, being abolished by NOSs-inhibitor NG-monomethyl-L-arginine (L-NMMA). A SIN-1-induced negative inotropism was found in presence of SOD. NOS inhibition by L-N5-N-iminoethyl-L-ornithine (L-NIO) and L-NMMA confirmed the NO-mediated negative inotropic influence on cardiac performance. In contrast, in C. aceratus, L-arginine elicited a positive inotropism. SIN-1 induced a negative inotropism, which disappeared in presence of SOD, indicating peroxynitrite involvement. Cardiac performance was unaffected by L-NIO and L-NIL. NO signaling acted via a cGMP-independent mechanism. This high conservation degree of NOS localization pattern and signaling highlights its importance for cardiac biology.

  9. A Handbook on File Structuring.

    ERIC Educational Resources Information Center

    Shapiro, Robert M.; And Others

    An initial attempt at presenting a coherent approach to the design and analysis of file structures include a discussion of the relative effeciency of different file implementations as a function of usage statistics. The fundamental differences between item and descriptor-organized files are discussed in terms of input-output requirements.…

  10. Functional significance of differential eNOS translocation

    PubMed Central

    Sánchez, Fabiola A.; Savalia, Nirav B.; Durán, Ricardo G.; Lal, Brajesh K.; Boric, Mauricio P.; Durán, Walter N.

    2006-01-01

    Nitric oxide (NO) regulates flow and permeability. ACh and platelet-activating factor (PAF) lead to endothelial NO synthase (eNOS) phosphorylation and NO release. While ACh causes only vasodilation, PAF induces vasoconstriction and hyperpermeability. The key differential signaling mechanisms for discriminating between vasodilation and hyperpermeability are unknown. We tested the hypothesis that differential translocation may serve as a regulatory mechanism of eNOS to determine specific vascular responses. We used ECV-304 cells permanently transfected with eNOS-green fluorescent protein (ECVeNOS-GFP) and demonstrated that the agonists activate eNOS and reproduce their characteristic endothelial permeability effects in these cells. We evaluated eNOS localization by lipid raft analysis and immunofluorescence microscopy. After PAF and ACh, eNOS moves away from caveolae. eNOS distributes both in the plasma membrane and Golgi in control cells. ACh (10−5 M, 10−4 M) translocated eNOS preferentially to the trans-Golgi network (TGN) and PAF (10−7 M) preferentially to the cytosol. We suggest that PAF-induced eNOS translocation preferentially to cytosol reflects a differential signaling mechanism related to changes in permeability, whereas ACh-induced eNOS translocation to the TGN is related to vasodilation. PMID:16679407

  11. NASA Thesaurus Data File

    NASA Technical Reports Server (NTRS)

    2012-01-01

    The NASA Thesaurus contains the authorized NASA subject terms used to index and retrieve materials in the NASA Aeronautics and Space Database (NA&SD) and NASA Technical Reports Server (NTRS). The scope of this controlled vocabulary includes not only aerospace engineering, but all supporting areas of engineering and physics, the natural space sciences (astronomy, astrophysics, planetary science), Earth sciences, and the biological sciences. The NASA Thesaurus Data File contains all valid terms and hierarchical relationships, USE references, and related terms in machine-readable form. The Data File is available in the following formats: RDF/SKOS, RDF/OWL, ZThes-1.0, and CSV/TXT.

  12. NASA Thesaurus Data File

    NASA Technical Reports Server (NTRS)

    2012-01-01

    The NASA Thesaurus contains the authorized NASA subject terms used to index and retrieve materials in the NASA Aeronautics and Space Database (NA&SD) and NASA Technical Reports Server (NTRS). The scope of this controlled vocabulary includes not only aerospace engineering, but all supporting areas of engineering and physics, the natural space sciences (astronomy, astrophysics, planetary science), Earth sciences, and the biological sciences. The NASA Thesaurus Data File contains all valid terms and hierarchical relationships, USE references, and related terms in machine-readable form. The Data File is available in the following formats: RDF/SKOS, RDF/OWL, ZThes-1.0, and CSV/TXT.

  13. Accessing files in an internet - The Jade file system

    NASA Technical Reports Server (NTRS)

    Rao, Herman C.; Peterson, Larry L.

    1993-01-01

    Jade is a new distribution file system that provides a uniform way to name and access files in an internet environment. It makes two important contributions. First, Jade is a logical system that integrates a heterogeneous collection of existing file systems, where heterogeneous means that the underlying file systems support different file access protocols. Jade is designed under the restriction that the underlying file system may not be modified. Second, rather than providing a global name space, Jade permits each user to define a private name space. These private name spaces support two novel features: they allow multiple file systems to be mounted under one directory, and they allow one logical name space to mount other logical name spaces. A prototype of the Jade File System was implemented on Sun Workstations running Unix. It consists of interfaces to the Unix file system, the Sun Network File System, the Andrew File System, and FTP. This paper motivates Jade's design, highlights several aspects of its implementation, and illustrates applications that can take advantage of its features.

  14. Accessing files in an Internet: The Jade file system

    NASA Technical Reports Server (NTRS)

    Peterson, Larry L.; Rao, Herman C.

    1991-01-01

    Jade is a new distribution file system that provides a uniform way to name and access files in an internet environment. It makes two important contributions. First, Jade is a logical system that integrates a heterogeneous collection of existing file systems, where heterogeneous means that the underlying file systems support different file access protocols. Jade is designed under the restriction that the underlying file system may not be modified. Second, rather than providing a global name space, Jade permits each user to define a private name space. These private name spaces support two novel features: they allow multiple file systems to be mounted under one directory, and they allow one logical name space to mount other logical name spaces. A prototype of the Jade File System was implemented on Sun Workstations running Unix. It consists of interfaces to the Unix file system, the Sun Network File System, the Andrew File System, and FTP. This paper motivates Jade's design, highlights several aspects of its implementation, and illustrates applications that can take advantage of its features.

  15. 75 FR 39206 - Marine Mammals; File Nos. 87-1851 and 555-1870

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-08

    ... robust survival model and to apply the new surgical protocols in the field if the pilot study proves... (50 CFR part 216). Permit No. 87-1851-02 authorizes tagging studies and physiological research on... capture participate in a metabolic study in addition to currently permitted procedures. The amendment...

  16. 78 FR 2955 - Marine Mammals; File Nos. 14451, 14353, and 13846

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... authorization under the MMPA to take 205 false killer whales (Pseudorca crassidens) in Pacific waters off of Hawaii. Insular Hawaiian false killer whales were listed as endangered under the ESA in November 2012; therefore, the permit holder now requires authorization under the ESA to take Insular Hawaiian false killer...

  17. 76 FR 68161 - Marine Mammals; File Nos. 16163, 16160, and 15569

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-03

    ...), killer whale (Orcinus orca) Southern Resident stock, and sperm whale (Physeter macrocephalus). The false killer whale (Pseudorca crassidens) Hawaiian insular stock is proposed for listing under the ESA. Seven...-cup tagged. Ultrasound sampling would be directed at killer whales including the Southern Resident...

  18. 78 FR 30872 - Marine Mammals; File Nos. 14451, 14353, and 13846

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ..., and behavioral observations. The permit includes authorization under the MMPA to take 205 false killer whales (Pseudorca crassidens) in Pacific waters off of Hawaii. Insular Hawaiian false killer whales were... authorization under the ESA to take Insular Hawaiian false killer whales. The permit amendment, Permit No. 14451...

  19. 77 FR 35657 - Marine Mammals; File Nos. 16163, 16160, and 15569

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... Investigator) , 2725 Montlake Blvd. East, Seattle, WA 98112-2097; The Whale Museum (Jenny Atkinson, Responsible Party) , P.O. Box 945, Friday Harbor, WA 98250; and The Center for Whale Research (CWR; Kenneth C... suction- cup tagged. Ultrasound sampling will be directed at killer whales including the Southern...

  20. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or...) Except for documents electronically filed under paragraph (c) of this section, a document is timely filed upon delivery to the Federal Election Commission, 999 E Street, NW., Washington, DC 20463; or...

  1. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or...) Except for documents electronically filed under paragraph (c) of this section, a document is timely filed upon delivery to the Federal Election Commission, 999 E Street, NW., Washington, DC 20463; or...

  2. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or...) Except for documents electronically filed under paragraph (c) of this section, a document is timely filed upon delivery to the Federal Election Commission, 999 E Street, NW., Washington, DC 20463; or...

  3. 78 FR 17394 - Filing via the Internet; Electronic Tariff Filings; Revisions to Electric Quarterly Report Filing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Filing via the Internet; Electronic Tariff Filings; Revisions to Electric Quarterly Report Filing Process; Notice of Technical Conference Take notice that on April 16, 2013,...

  4. Nitric oxide pathway genes (NOS1AP and NOS1) are involved in PTSD severity, depression, anxiety, stress and resilience.

    PubMed

    Bruenig, Dagmar; Morris, Charles P; Mehta, Divya; Harvey, Wendy; Lawford, Bruce; Young, Ross McD; Voisey, Joanne

    2017-08-20

    The nitric oxide pathway in the hippocampus is involved in the biological stress response with detrimental consequences to cells and HPA axis feedback. Hippocampal atrophy and HPA axis feedback dysfunction are associated with posttraumatic stress disorder (PTSD). This study systematically investigates two genes of the nitric oxide pathway NOS1AP and NOS1 for a potential involvement in PTSD, comorbidities and resilience. A cohort of age and gender matched Vietnam veterans including trauma-exposed cases and controls was recruited and comprehensively assessed (n=299). A total of 49 NOS1AP and 16 NOS1 polymorphisms were analysed and genotypes correlated with gold standard clinical measures to assess PTSD severity and related phenotypes (depression, anxiety, stress, resilience) based on diagnostic status. Multiple NOS1AP polymorphisms were associated across all measures, and NOS1 polymorphisms were associated with PTSD severity, stress and resilience. The GG genotype of NOS1 polymorphism rs10744891 was associated with PTSD severity (surviving multiple correction) while the combined TT-TG genotypes were associated with resilience (p=0.005; p=0.033, respectively). This study indicates that NOS1AP and NOS1 from the nitric oxide pathway are likely to play a key role in PTSD, its comorbidities and resilience. Given the essential role of NOS1AP and NOS1 in stress response they may be reliable targets for screening and intervention strategies. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

  5. "The Bean Files."

    ERIC Educational Resources Information Center

    Haq, Krystyna; Longnecker, Nancy; Hickey, Ruth

    1999-01-01

    Describes classroom use and effectiveness of "The Bean Files," an internet package that uses humorous stories to introduce students to life on a wheat-sheep farm in the Mediterranean climate areas of Australia. The focus of the program is on the role of legume-cereal rotations in the farming system and the science underpinning this…

  6. "The Bean Files."

    ERIC Educational Resources Information Center

    Haq, Krystyna; Longnecker, Nancy; Hickey, Ruth

    1999-01-01

    Describes classroom use and effectiveness of "The Bean Files," an internet package that uses humorous stories to introduce students to life on a wheat-sheep farm in the Mediterranean climate areas of Australia. The focus of the program is on the role of legume-cereal rotations in the farming system and the science underpinning this…

  7. The Global File System

    NASA Technical Reports Server (NTRS)

    Soltis, Steven R.; Ruwart, Thomas M.; OKeefe, Matthew T.

    1996-01-01

    The global file system (GFS) is a prototype design for a distributed file system in which cluster nodes physically share storage devices connected via a network-like fiber channel. Networks and network-attached storage devices have advanced to a level of performance and extensibility so that the previous disadvantages of shared disk architectures are no longer valid. This shared storage architecture attempts to exploit the sophistication of storage device technologies whereas a server architecture diminishes a device's role to that of a simple component. GFS distributes the file system responsibilities across processing nodes, storage across the devices, and file system resources across the entire storage pool. GFS caches data on the storage devices instead of the main memories of the machines. Consistency is established by using a locking mechanism maintained by the storage devices to facilitate atomic read-modify-write operations. The locking mechanism is being prototyped in the Silicon Graphics IRIX operating system and is accessed using standard Unix commands and modules.

  8. The New Resource File

    ERIC Educational Resources Information Center

    Luck, Donald D.

    2011-01-01

    The development of the resource file is a common experience in teacher preparation programs. The author examines strategies for transforming what has been a project composed of physical resources to one emphasizing digital resources. Methods for finding, tagging, storing and retrieving resources are explored.

  9. Master File Feasibility Study.

    ERIC Educational Resources Information Center

    Broadbent, William A.

    The three purposes for which this study was conducted involved collection and summarization of otherwise unavailable data on student characteristics and aspirations, editing and summarization of reading test data collected over the past six years, and testing of the feasibility and utility of a computerized master file combining student data…

  10. Illegal File Sharing 101

    ERIC Educational Resources Information Center

    Wada, Kent

    2008-01-01

    Much of higher education's unease arises from the cost of dealing with illegal file sharing. Illinois State University, for example, calculated a cost of $76 to process a first claim of copyright infringement and $146 for a second. Responses range from simply passing along claims to elaborate programs architected with specific goals in mind.…

  11. Single nucleotide polymorphisms in the NOS2 and NOS3 genes are associated with exhaled nitric oxide.

    PubMed

    Dahgam, Santosh; Nyberg, Fredrik; Modig, Lars; Naluai, Asa Torinsson; Olin, Anna-Carin

    2012-03-01

    Polymorphisms in nitric oxide synthase genes (NOS1, NOS2, and NOS3) have been suggested to have a major impact on fraction of exhaled nitric oxide (FENO), a biomarker of airway inflammation. However, the genetic contribution of NOS polymorphisms to FENO is not fully understood. The aim of this study was to investigate comprehensively the association between single nucleotide polymorphisms (SNPs) in all three NOS genes and FENO in an adult population, and to assess whether such associations are modified by asthma or atopy. In 1737 adults from a Swedish general population sample, FENO was measured and genetic variation in the NOS genes was assessed using 49 SNPs. The genetic effect of NOS polymorphisms on FENO, asthma, and atopy was estimated using multiple regression methods. In a multi-SNP model based on stepwise regression analysis, two SNPs in NOS2 and one in NOS3 showed independent associations with levels of FENO. For NOS2 SNP rs9901734, subjects had 5.3% (95% CI 1.0% to 9.7%) higher levels of FENO per G allele, and for rs3729508, subjects with CC or CT genotypes had 9.4% (95% CI 3.1% to 15.2%) higher levels compared with TT. For NOS3 SNP rs7830, subjects with GT or TT had 5.6% (95% CI 0.4% to 11.1%) higher levels than GG; the genetic effect of this SNP was stronger in asthmatics (21.9%, 95% CI 4.6% to 42.0%). These results suggest that NOS2 is the major NOS gene determining variability in exhaled nitric oxide in the healthy adult population, while NOS3 may play a more important role in asthmatic adults.

  12. The dual role of iNOS in cancer☆

    PubMed Central

    Vanini, Frederica; Kashfi, Khosrow; Nath, Niharika

    2015-01-01

    Nitric oxide (NO) is one of the 10 smallest molecules found in nature. It is a simple gaseous free radical whose predominant functions is that of a messenger through cGMP. In mammals, NO is synthesized by the enzyme nitric oxide synthase (NOS) of which there are three isoforms. Neuronal (nNOS, NOS1) and endothelial (eNOS, NOS3) are constitutive calcium-dependent forms of the enzyme that regulate neural and vascular function respectively. The third isoform (iNOS, NOS2), is calcium-independent and is inducible. In many tumors, iNOS expression is high, however, the role of iNOS during tumor development is very complex and quite perplexing, with both promoting and inhibiting actions having been described. This review will aim to summarize the dual actions of iNOS-derived NO showing that the microenvironment of the tumor is a contributing factor to these observations and ultimately to cellular outcomes. PMID:26335399

  13. In situ observation of reactive oxygen species forming on oxygen-evolving iridium surfaces† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc04622c Click here for additional data file.

    PubMed Central

    Pfeifer, Verena; Velasco Vélez, Juan J.; Piccinin, Simone; Hävecker, Michael; Knop-Gericke, Axel; Schlögl, Robert

    2017-01-01

    Water splitting performed in acidic media relies on the exceptional performance of iridium-based materials to catalyze the oxygen evolution reaction (OER). In the present work, we use in situ X-ray photoemission and absorption spectroscopy to resolve the long-standing debate about surface species present in iridium-based catalysts during the OER. We find that the surface of an initially metallic iridium model electrode converts into a mixed-valent, conductive iridium oxide matrix during the OER, which contains OII– and electrophilic OI– species. We observe a positive correlation between the OI– concentration and the evolved oxygen, suggesting that these electrophilic oxygen sites may be involved in catalyzing the OER. We can understand this observation by analogy with photosystem II; their electrophilicity renders the OI– species active in O–O bond formation, i.e. the likely potential- and rate-determining step of the OER. The ability of amorphous iridium oxyhydroxides to easily host such reactive, electrophilic species can explain their superior performance when compared to plain iridium metal or crystalline rutile-type IrO2. PMID:28507666

  14. Ivabradine Prevents Low Shear Stress Induced Endothelial Inflammation and Oxidative Stress via mTOR/eNOS Pathway

    PubMed Central

    Li, Bing; Zhang, Junxia; Wang, Zhimei; Chen, Shaoliang

    2016-01-01

    Ivabradine not only reduces heart rate but has other cardiac and vascular protective effects including anti-inflammation and anti-oxidation. Since endothelial nitric oxide synthase (eNOS) is a crucial enzyme in maintaining endothelial activity, we aimed to investigate the impact of ivabradine in low shear stress (LSS) induced inflammation and endothelial injury and the role of eNOS played in it. Endothelial cells (ECs) were subjected to LSS at 2dyne/cm2, with 1 hour of ivabradine (0.04μM) or LY294002 (10μM) pre-treatment. The mRNA expression of IL-6, VCAM-1 along with eNOS were measured by QPCR. Reactive oxygen species (ROS) was detected by dihydroethidium (DHE) and DCF, and protein phosphorylation was detected by western blot. It demonstrated that ivabradine decreased LSS induced inflammation and oxidative stress in endothelial cells. Western blot showed reduced rictor and Akt-Ser473 as well as increased eNOS-Thr495 phosphorylation. However, mTORC1 pathway was only increased when LSS applied within 30 minutes. These effects were reversed by ivabradine. It would appear that ivabradine diminish ROS generation by provoking mTORC2/Akt phosphorylation and repressing mTORC1 induced eNOS-Thr495 activation. These results together suggest that LSS induced endothelial inflammation and oxidative stress are suppressed by ivabradine via mTORC2/Akt activation and mTORC1/eNOS reduction. PMID:26890696

  15. Ivabradine Prevents Low Shear Stress Induced Endothelial Inflammation and Oxidative Stress via mTOR/eNOS Pathway.

    PubMed

    Li, Bing; Zhang, Junxia; Wang, Zhimei; Chen, Shaoliang

    2016-01-01

    Ivabradine not only reduces heart rate but has other cardiac and vascular protective effects including anti-inflammation and anti-oxidation. Since endothelial nitric oxide synthase (eNOS) is a crucial enzyme in maintaining endothelial activity, we aimed to investigate the impact of ivabradine in low shear stress (LSS) induced inflammation and endothelial injury and the role of eNOS played in it. Endothelial cells (ECs) were subjected to LSS at 2dyne/cm2, with 1 hour of ivabradine (0.04μM) or LY294002 (10μM) pre-treatment. The mRNA expression of IL-6, VCAM-1 along with eNOS were measured by QPCR. Reactive oxygen species (ROS) was detected by dihydroethidium (DHE) and DCF, and protein phosphorylation was detected by western blot. It demonstrated that ivabradine decreased LSS induced inflammation and oxidative stress in endothelial cells. Western blot showed reduced rictor and Akt-Ser473 as well as increased eNOS-Thr495 phosphorylation. However, mTORC1 pathway was only increased when LSS applied within 30 minutes. These effects were reversed by ivabradine. It would appear that ivabradine diminish ROS generation by provoking mTORC2/Akt phosphorylation and repressing mTORC1 induced eNOS-Thr495 activation. These results together suggest that LSS induced endothelial inflammation and oxidative stress are suppressed by ivabradine via mTORC2/Akt activation and mTORC1/eNOS reduction.

  16. Icariin improves eNOS/NO pathway to prohibit the atherogenesis of apolipoprotein E-null mice.

    PubMed

    Xiao, Hong-Bo; Sui, Guo-Guang; Lu, Xiang-Yang

    2016-12-22

    Impaired endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) pathway induces atherogenesis. The present study examined whether icariin improves the eNOS/NO pathway to prohibit the atherogenesis of apolipoprotein E-null (ApoE(-/-)) mice. In vitro, primary human umbilical vein endothelial cells (HUVECs) were randomly divided into 7 groups: control; vehicle; icariin 10; lyphosphatidylcholine (LPC) group; LPC + icariin 1; LPC + icariin 3; and LPC + icariin 10. In vivo, 80 mice were separated randomly into 4 groups (n = 20): control, ApoE(-/-), ApoE(-/-) + icariin 10, and ApoE(-/-) + icariin 30. ApoE(-/-) mice had significantly more atherosclerosis in the aortic root together with increased aortic ROS production, body mass, plasma triglyceride (TG) and total cholesterol (TC) concentration, decreased aortic eNOS expression, and plasma NO concentration. LPC (10 μg/mL) treatment induced a big decline in NO level in the conditioned medium and eNOS expression, and an increase in intracellular reactive oxygen species (ROS) production in HUVECs. Icariin treatment decreased atherogenesis, ROS production, body mass, plasma TG concentration, and plasma TC concentration, and increased NO concentration and eNOS expression. These findings suggested icariin could improve eNOS/NO-pathway to prohibit the atherogenesis of apolipoprotein E-null mice by restraining oxidative stress.

  17. Expression of the nos gene and firefly flashing: a test of the nitric-oxide-mediated flash control model.

    PubMed

    Ohtsuki, Hajime; Yokoyama, Jun; Ohba, Nobuyoshi; Ohmiya, Yoshihiro; Kawata, Masakado

    2014-04-19

    Fireflies (Coleoptera: Lampyridae) emit various types of light that differ among species and populations of the same species. Their lights are assumed to be biological properties that play important ecological and evolutionary roles. Some species in the Lampyridae emit periodic luminescence, the patterns of which are characterized by species-specific intervals. In previous work, it was predicted that the nitric oxide (NO) regulates the oxygen supply required for the bioluminescence reaction of fireflies. Here, the expression of the NO synthase (NOS) mRNA in some fireflies was examined to verify the predictive model of nitric-oxide-mediated flash control in these insects. The expression of the nos gene in the lantern organ was observed not only in nocturnal flashing species but also in diurnal non-flashing species. It was shown that the expression levels of nos were higher in the lantern of Luciola cruciata (Motschulsky) larvae, which that emits continuous light, than in other body parts, although expression in the lantern of the adults, who flash periodically, was not high. Furthermore, there was no significant difference in expression levels among adults of Luciola cruciata characterized by different flashing intervals. The data do not support the model of an NO-mediated flash control mechanism, during which oxygen becomes available for the luciferin-luciferase reaction through NO-mediated inhibition of mitochondrial respiration. It is also indicated that flash patterns do not co-vary with NOS production. However, high nos expression in the larval lantern suggests that NO may play a role in producing continuous light by functioning as a neurotransmitter signal for bioluminescence.

  18. Expression of the nos gene and Firefly Flashing: A Test of the Nitric-Oxide-Mediated Flash Control Model

    PubMed Central

    Ohtsuki, Hajime; Yokoyama, Jun; Ohba, Nobuyoshi; Ohmiya, Yoshihiro; Kawata, Masakado

    2014-01-01

    Fireflies (Coleoptera: Lampyridae) emit various types of light that differ among species and populations of the same species. Their lights are assumed to be biological properties that play important ecological and evolutionary roles. Some species in the Lampyridae emit periodic luminescence, the patterns of which are characterized by species-specific intervals. In previous work, it was predicted that the nitric oxide (NO) regulates the oxygen supply required for the bioluminescence reaction of fireflies. Here, the expression of the NO synthase (NOS) mRNA in some fireflies was examined to verify the predictive model of nitric-oxide-mediated flash control in these insects. The expression of the nos gene in the lantern organ was observed not only in nocturnal flashing species but also in diurnal non-flashing species. It was shown that the expression levels of nos were higher in the lantern of Luciola cruciata (Motschulsky) larvae, which that emits continuous light, than in other body parts, although expression in the lantern of the adults, who flash periodically, was not high. Furthermore, there was no significant difference in expression levels among adults of Luciola cruciata characterized by different flashing intervals. The data do not support the model of an NO-mediated flash control mechanism, during which oxygen becomes available for the luciferin-luciferase reaction through NO-mediated inhibition of mitochondrial respiration. It is also indicated that flash patterns do not co-vary with NOS production. However, high nos expression in the larval lantern suggests that NO may play a role in producing continuous light by functioning as a neurotransmitter signal for bioluminescence. PMID:25373203

  19. iNOS-dependent sweating and eNOS-dependent cutaneous vasodilation are evident in younger adults, but are diminished in older adults exercising in the heat.

    PubMed

    Fujii, Naoto; Meade, Robert D; Alexander, Lacy M; Akbari, Pegah; Foudil-Bey, Imane; Louie, Jeffrey C; Boulay, Pierre; Kenny, Glen P

    2016-02-01

    Nitric oxide synthase (NOS) contributes to sweating and cutaneous vasodilation during exercise in younger adults. We hypothesized that endothelial NOS (eNOS) and neuronal NOS (nNOS) mediate NOS-dependent sweating, whereas eNOS induces NOS-dependent cutaneous vasodilation in younger adults exercising in the heat. Further, aging may upregulate inducible NOS (iNOS), which may attenuate sweating and cutaneous vasodilator responses. We hypothesized that iNOS inhibition would augment sweating and cutaneous vasodilation in exercising older adults. Physically active younger (n = 12, 23 ± 4 yr) and older (n = 12, 60 ± 6 yr) adults performed two 30-min bouts of cycling at a fixed rate of metabolic heat production (400 W) in the heat (35°C). Sweat rate and cutaneous vascular conductance (CVC) were evaluated at four intradermal microdialysis sites with: 1) lactated Ringer (control), 2) nNOS inhibitor (nNOS-I, NPLA), 3) iNOS inhibitor (iNOS-I, 1400W), or 4) eNOS inhibitor (eNOS-I, LNAA). In younger adults during both exercise bouts, all inhibitors decreased sweating relative to control, albeit a lower sweat rate was observed at iNOS-I compared with eNOS-I and nNOS-I sites (all P < 0.05). CVC at the eNOS-I site was lower than control in younger adults throughout the intermittent exercise protocol (all P < 0.05). In older adults, there were no differences between control and iNOS-I sites for sweating and CVC during both exercise bouts (all P > 0.05). We show that iNOS and eNOS are the main contributors to NOS-dependent sweating and cutaneous vasodilation, respectively, in physically active younger adults exercising in the heat, and that iNOS inhibition does not alter sweating or cutaneous vasodilation in exercising physically active older adults. Copyright © 2016 the American Physiological Society.

  20. iNOS-dependent sweating and eNOS-dependent cutaneous vasodilation are evident in younger adults, but are diminished in older adults exercising in the heat

    PubMed Central

    Fujii, Naoto; Meade, Robert D.; Alexander, Lacy M.; Akbari, Pegah; Foudil-bey, Imane; Louie, Jeffrey C.; Boulay, Pierre

    2015-01-01

    Nitric oxide synthase (NOS) contributes to sweating and cutaneous vasodilation during exercise in younger adults. We hypothesized that endothelial NOS (eNOS) and neuronal NOS (nNOS) mediate NOS-dependent sweating, whereas eNOS induces NOS-dependent cutaneous vasodilation in younger adults exercising in the heat. Further, aging may upregulate inducible NOS (iNOS), which may attenuate sweating and cutaneous vasodilator responses. We hypothesized that iNOS inhibition would augment sweating and cutaneous vasodilation in exercising older adults. Physically active younger (n = 12, 23 ± 4 yr) and older (n = 12, 60 ± 6 yr) adults performed two 30-min bouts of cycling at a fixed rate of metabolic heat production (400 W) in the heat (35°C). Sweat rate and cutaneous vascular conductance (CVC) were evaluated at four intradermal microdialysis sites with: 1) lactated Ringer (control), 2) nNOS inhibitor (nNOS-I, NPLA), 3) iNOS inhibitor (iNOS-I, 1400W), or 4) eNOS inhibitor (eNOS-I, LNAA). In younger adults during both exercise bouts, all inhibitors decreased sweating relative to control, albeit a lower sweat rate was observed at iNOS-I compared with eNOS-I and nNOS-I sites (all P < 0.05). CVC at the eNOS-I site was lower than control in younger adults throughout the intermittent exercise protocol (all P < 0.05). In older adults, there were no differences between control and iNOS-I sites for sweating and CVC during both exercise bouts (all P > 0.05). We show that iNOS and eNOS are the main contributors to NOS-dependent sweating and cutaneous vasodilation, respectively, in physically active younger adults exercising in the heat, and that iNOS inhibition does not alter sweating or cutaneous vasodilation in exercising physically active older adults. PMID:26586908

  1. eNOS-Dependent Antisenscence Effect of a Calcium Channel Blocker in Human Endothelial Cells

    PubMed Central

    Hayashi, Toshio; Yamaguchi, Tomoe; Sakakibara, Yasufumi; Taguchi, Kumiko; Maeda, Morihiko; Kuzuya, Masafumi; Hattori, Yuichi

    2014-01-01

    Senescence of vascular endothelial cells is an important contributor to the pathogenesis of age-associated vascular disorders such as atherosclerosis. We investigated the effects of antihypertensive agents on high glucose-induced cellular senescence in human umbilical venous endothelial cells (HUVECs). Exposure of HUVECs to high glucose (22 mM) for 3 days increased senescence-associated- β-galactosidase (SA-β-gal) activity, a senescence marker, and decreased telomerase activity, a replicative senescence marker. The calcium channel blocker nifedipine, but not the β1-adrenergic blocking agent atenolol or the angiotensin-converting enzyme inhibitor perindopril, reduced SA-β-gal positive cells and prevented a decrease in telomerase activity in a high-glucose environment. This beneficial effect of nifedipine was associated with reduced reactive oxygen species (ROS) and increased endothelial nitric oxide synthase (eNOS) activity. Thus, nifedipine prevented high glucose-induced ROS generation and increased basal eNOS phosphorylation level at Ser-1177. Treatment with NG-nitro-L-arginine (L-NAME) and transfection of small interfering RNA (siRNA) targeting eNOS eliminated the anti-senscence effect of nifedipine. These results demonstrate that nifedipine can prevent endothelial cell senescence in an eNOS-dependent manner. The anti-senescence action of nifedipine may represent a novel mechanism by which it protects against atherosclerosis. PMID:24520379

  2. Diminished Neurogenic Femoral Artery Vasoconstrictor Response in a Zucker Obese Rat Model: Differential Regulation of NOS and COX Derivatives

    PubMed Central

    Martínez, Ana Cristina; Hernández, Medardo; Novella, Susana; Martínez, María Pilar; Pagán, Rosa María; Hermenegildo, Carlos; García-Sacristán, Albino; Prieto, Dolores; Benedito, Sara

    2014-01-01

    Objective Peripheral arterial disease is one of the macrovascular complications of type 2 diabetes mellitus. This study addresses femoral artery regulation in a prediabetic model of obese Zucker rats (OZR) by examining cross-talk between endothelial and neural factors. Methods and Results Arterial preparations from lean (LZR) and OZR were subjected to electrical field stimulation (EFS) on basal tone. Nitric oxide synthase (NOS) and cyclooxygenase (COX) isoform expression patterns were determined by immunohistochemical labelling and Western blotting. Results indicate significantly reduced noradrenergic contractions in preparations from OZR compared with those of LZR. Functional inhibition of endothelial NOS (eNOS) indicated a predominant role of this isoform in LZR and its modified activity in OZR. Neural (nNOS) and inducible NOS (iNOS) were activated and their expression was higher in femoral arteries from OZR. Neurotransmission modulated by large-conductance Ca2+-activated (BKCa) or voltage-dependent (KV) K+ channels did not seem compromised in the obese animals. Endothelial COX-1 and COX-2 were expressed in LZR and an additional adventitial location of COX-2 was also observed in OZR, explaining the higher COX-2 protein levels detected in this group. Prostanoids derived from both isoforms helped maintain vasoconstriction in LZR while in OZR only COX-2 was active. Superoxide anion inhibition reduced contractions in endothelium-intact arteries from OZR. Conclusions Endothelial dysfunction led to reduced neurogenic vasoconstriction in femoral arteries from OZR. In a setting of obesity, NO-dependent nNOS and iNOS dilation activity could be an alternative mechanism to offset COX-2- and reactive oxygen species-mediated vasoconstriction, along with impaired endothelial NO relaxation. PMID:25216050

  3. Effect of melatonin and vitamin C on expression of endothelial NOS in heart of chronic alcoholic rats.

    PubMed

    Sönmez, M F; Narin, F; Akkuş, D; Ozdamar, S

    2009-07-01

    The aim of this study was to investigate the effects of melatonin and vitamin C on expression of endothelial nitric oxide synthase (NOS) in heart tissue of chronic alcoholic rats. Twenty-four adult male Wistar rats weighing 200-250 g were used in this study. Rats were divided into four groups. The first group served as control (n = 6). The second group was treated with ethanol (%7.2) for 28 days (n = 6), which was administered in artificial isocaloric diets. The third group was given ethanol and supplemented with 40 mg/kg vitamin C [intraperitoneally (i.p.)] (n = 6). The fourth group was given ethanol and supplemented with 4 mg/kg melatonin (i.p.) (n = 6). At the end of the experiment, rats were sacrificed and heart tissues were processed for immunohistochemistry analysis to endothelial NOS (eNOS). eNOS immunoreactivity showed heterogeneous distribution in control group. eNOS immunoreactivity was (+) in some myocytes and (++) in some others. Expression of eNOS in alcohol group was heterogeneous like control group but also stronger than that. Immunoreactivity was (+++) in myocytes near the epicardial zone and (++) in myocytes near the endocardium border. In melatonin and vitamin C-treated groups, eNOS immunoreactivity was diffuse and the intensity of reaction was (+++) in subepicardial region. However, eNOS immunoreactivity scores were weaker in these groups when compared with the alcohol group. Our results indicate that alleviation of oxidative stress by antioxidant therapy reduces reactive oxygen species-mediated nitric oxide inactivation.

  4. Relationships between polymorphisms in NOS3 and MPO genes, cigarette smoking and risk of post-menopausal breast cancer.

    PubMed

    Yang, Jun; Ambrosone, Christine B; Hong, Chi-Chen; Ahn, Jiyoung; Rodriguez, Carmen; Thun, Michael J; Calle, Eugenia E

    2007-06-01

    NOS3 and MPO genes encode endothelial nitric oxide synthase and myeloperoxidase (MPO), respectively, which generate nitric oxide and reactive oxygen species. Because cigarette smoking generates reactive species, we hypothesized that NOS3 and MPO polymorphisms could influence susceptibility to breast cancer, particularly among smokers. We examined the associations between NOS3 Glu298Asp and MPO G-463A polymorphisms and breast cancer risk by cigarette smoking among post-menopausal women in the American Cancer Society's Cancer Prevention Study II Nutrition Cohort. Included in this analysis were 502 women who provided blood samples and were diagnosed with breast cancer between 1992 and 2001 and 505 cancer-free controls who were matched to the cases by age, race/ethnicity and date of blood donation. Genotyping for NOS3 and MPO was performed using TaqMan, and unconditional logistic regression was used to compute odds ratios (ORs) and 95% confidence intervals (CIs). No statistically significant relationships were found between NOS3 and MPO genotypes and breast cancer risk. When considering smoking, variant NOS3 genotypes (GT and TT) were significantly associated with reduced breast cancer risk among never smokers (OR = 0.67, 95% CI = 0.45-0.99), but were associated with higher risk among ever smokers (OR = 1.59, 95% CI = 1.05-2.41) and 2-fold increase in risk for those who smoked >10 cigarettes per day (OR = 2.19, 95% CI = 1.21-3.97). NOS3 genotypes appeared to be associated with risk of post-menopausal breast cancer among smokers, supporting the hypothesis that subgroups of women based upon genetic profiles may be at higher risk of breast cancer when exposed to tobacco smoke.

  5. 76 FR 11762 - Marine Mammals; File No. 15330; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ...'' from the Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15530 from the list of available applications. These... East-West Highway, Room 13705, Silver Spring, MD 20910; phone (301)713-2289; fax...

  6. 75 FR 20565 - Marine Mammals; File No. 14636

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-20

    ...'' from the Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 14636 from the list of available applications. These..., Silver Spring, MD 20910; phone (301) 713-2289; fax (301) 713-0376; and Southwest Region, NMFS, 501...

  7. 76 FR 28421 - Marine Mammals; File No. 15646

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ...'' from the Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15646 from the list of available applications. These..., Silver Spring, MD 20910; phone (301) 713-2289; fax (301) 713-0376; Southwest Region, NMFS, 501 West...

  8. 76 FR 13603 - Marine Mammals; File No. 15748

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... Public Comment'' from the Features box on the Applications and Permits for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15748 from the list of available...-West Highway, Room 13705, Silver Spring, MD 20910; phone (301) 713-2289; fax (301) 713-0376; and...

  9. 77 FR 31585 - Marine Mammals; File No. 16388

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... National Oceanic and Atmospheric Administration RIN 0648-XB154 Marine Mammals; File No. 16388 AGENCY... Mammal Protection Act of 1972, as amended (MMPA; 16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as amended...

  10. 76 FR 72178 - Marine Mammals; File No. 14334

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-22

    ... National Oceanic and Atmospheric Administration RIN 0648-XP18 Marine Mammals; File No. 14334 AGENCY... Marine Mammal Protection Act of 1972, as amended (16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as amended...

  11. 78 FR 53732 - Marine Mammals; File No. 14325

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-30

    ... National Oceanic and Atmospheric Administration RIN 0648-XC010 Marine Mammals; File No. 14325 AGENCY... amendment to Permit No. 14325-02 is requested under the authority of the Marine Mammal Protection Act of... mammals (50 CFR part 216), the Endangered Species Act of 1973, as amended (16 U.S.C. 1531 et seq.), the...

  12. 77 FR 40859 - Marine Mammals; File No. 14097

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-11

    ... National Oceanic and Atmospheric Administration RIN 0648-XX47 Marine Mammals; File No. 14097 AGENCY... Marine Mammal Protection Act of 1972, as amended (16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as...

  13. 78 FR 21113 - Marine Mammals; File No. 17845

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-09

    ... National Oceanic and Atmospheric Administration RIN 0648-XC599 Marine Mammals; File No. 17845 AGENCY... Mammal Protection Act of 1972, as amended (MMPA; 16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as amended...

  14. 76 FR 67151 - Marine Mammals; File No. 13927

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... National Oceanic and Atmospheric Administration RIN 0648-XA774 Marine Mammals; File No. 13927 AGENCY... the activities requested by the applicant has been issued under the authority of the Marine Mammal... importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as amended (ESA; 16 U.S.C...

  15. 75 FR 39665 - Marine Mammals; File No. 14791

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-12

    ... National Oceanic and Atmospheric Administration RIN 0648-XX38 Marine Mammals; File No. 14791 AGENCY... authority of the Marine Mammal Protection Act of 1972, as amended (16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973...

  16. 76 FR 329 - Marine Mammals; File No. 14330

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-04

    ... National Oceanic and Atmospheric Administration RIN 0648-XA122 Marine Mammals; File No. 14330 AGENCY... Marine Mammal Protection Act of 1972, as amended (16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as amended...

  17. 77 FR 25963 - Marine Mammals; File No. 14325

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-02

    ...-XC010 Marine Mammals; File No. 14325 AGENCY: National Marine Fisheries Service (NMFS), National Oceanic... Marine Mammal Protection Act of 1972, as amended (16 U.S.C. 1361 et seq.), the regulations governing the taking and importing of marine mammals (50 CFR part 216), the Endangered Species Act of 1973, as amended...

  18. 77 FR 27441 - Marine Mammals; File No. 13927

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... National Oceanic and Atmospheric Administration RIN 0648-XA774 Marine Mammals; File No. 13927 AGENCY... amendment to Permit No. 13927 is requested under the authority of the Marine Mammal Protection Act of 1972... mammals (50 CFR part 216), the Endangered Species Act of 1973, as amended (16 U.S.C. 1531 et seq.), and...

  19. 77 FR 20810 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-06

    ... Sequent 39754 & 39755 Capacity Release Negotiated Rate Agreement filing to be effective 5/1/2012. Filed... Capacity Release Negotiated Rate Agreement filing to be effective 4/1/2012. Filed Date: 3/30/12. Accession...: Bison Pipeline LLC. Description: Bison Pipeline LLC Company Use Gas Annual Report. Filed Date: 3/30/12...

  20. 48 CFR 4.802 - Contract files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Contract files. 4.802... MATTERS Government Contract Files 4.802 Contract files. (a) A contract file should generally consist of— (1) The contracting office contract file, that documents the basis for the acquisition and the...

  1. 48 CFR 4.802 - Contract files.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Contract files. 4.802... MATTERS Government Contract Files 4.802 Contract files. (a) A contract file should generally consist of— (1) The contracting office contract file, that documents the basis for the acquisition and the award...

  2. Break up a File with EDLIN.

    ERIC Educational Resources Information Center

    Koga, James S.

    Online searchers who download large amounts of data into a single ASCII file often have difficulty fitting that large file into their MS-DOS word processors for post-processing. This paper gives step-by-step instructions for using EDLIN, a text editor for DOS files, to break up a large downloaded file into smaller files. (1 reference) (Author/EW)

  3. 75 FR 50757 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-17

    ..., Inc. submits tariff filing per 154.204: DTI--Non-Conforming Service Agreements to be effective 8/30... Line Company, LLC submits tariff filing per 154.403: S-2 Tracker Filing to be effective 8/1/2010. Filed.... Description: Transwestern Pipeline Company, LLC submits its baseline tariff filing of its FERC Gas...

  4. 12 CFR 5.4 - Filing required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CORPORATE ACTIVITIES Rules of General Applicability § 5.4 Filing required. (a) Filing. A depository institution shall file an application or notice with the OCC to engage in corporate activities and... advise an applicant through a pre-filing communication to send the filing or submission directly to...

  5. 77 FR 53882 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    .... Filed Date: 8/23/12. Accession Number: 20120823-5123. Comments Due: 5 p.m. ET 9/4/12. Docket Numbers... Release Filing to be effective 9/25/2012. Filed Date: 8/24/12. Accession Number: 20120824-5042. Comments...: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676...

  6. 78 FR 76291 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-17

    ... Pipeline Company, L.L.C. Description: Revise System Map to be effective 1/1/2014. Filed Date: 12/4/13... Approval of Stipulation and Agreement. Filed Date: 12/4/13. Accession Number: 20131204-5130. Comments Due... qualifying facilities filings can be found at: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For...

  7. Effects of Paracetamol on NOS, COX, and CYP Activity and on Oxidative Stress in Healthy Male Subjects, Rat Hepatocytes, and Recombinant NOS

    PubMed Central

    Trettin, Arne; Böhmer, Anke; Suchy, Maria-Theresia; Probst, Irmelin; Staerk, Ulrich; Stichtenoth, Dirk O.; Frölich, Jürgen C.

    2014-01-01

    Paracetamol (acetaminophen) is a widely used analgesic drug. It interacts with various enzyme families including cytochrome P450 (CYP), cyclooxygenase (COX), and nitric oxide synthase (NOS), and this interplay may produce reactive oxygen species (ROS). We investigated the effects of paracetamol on prostacyclin, thromboxane, nitric oxide (NO), and oxidative stress in four male subjects who received a single 3 g oral dose of paracetamol. Thromboxane and prostacyclin synthesis was assessed by measuring their major urinary metabolites 2,3-dinor-thromboxane B2 and 2,3-dinor-6-ketoprostaglandin F1α, respectively. Endothelial NO synthesis was assessed by measuring nitrite in plasma. Urinary 15(S)-8-iso-prostaglanding F2α was measured to assess oxidative stress. Plasma oleic acid oxide (cis-EpOA) was measured as a marker of cytochrome P450 activity. Upon paracetamol administration, prostacyclin synthesis was strongly inhibited, while NO synthesis increased and thromboxane synthesis remained almost unchanged. Paracetamol may shift the COX-dependent vasodilatation/vasoconstriction balance at the cost of vasodilatation. This effect may be antagonized by increasing endothelial NO synthesis. High-dosed paracetamol did not increase oxidative stress. At pharmacologically relevant concentrations, paracetamol did not affect NO synthesis/bioavailability by recombinant human endothelial NOS or inducible NOS in rat hepatocytes. We conclude that paracetamol does not increase oxidative stress in humans. PMID:24799980

  8. A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense

    PubMed Central

    Jiang, Qiufen; Zhou, Zhi; Wang, Leilei; Wang, Lingling; Yue, Feng; Wang, Jingjing; Song, Linsheng

    2013-01-01

    Background Nitric oxide synthase (NOS) is responsible for synthesizing nitric oxide (NO) from L-arginine, and involved in multiple physiological functions. However, its immunological role in mollusc was seldom reported. Methodology In the present study, an NOS (CfNOS) gene was identified from the scallop Chlamys farreri encoding a polypeptide of 1486 amino acids. Its amino acid sequence shared 50.0~54.7, 40.7~47.0 and 42.5~44.5% similarities with vertebrate neuronal (n), endothelial (e) and inducible (i) NOSs, respectively. CfNOS contained PDZ, oxygenase and reductase domains, which resembled those in nNOS. The CfNOS mRNA transcripts expressed in all embryos and larvae after the 2-cell embryo stage, and were detectable in all tested tissues with the highest level in the gonad, and with the immune tissues hepatopancreas and haemocytes included. Moreover, the immunoreactive area of CfNOS distributed over the haemocyte cytoplasm and cell membrane. After LPS, β-glucan and PGN stimulation, the expression level of CfNOS mRNA in haemocytes increased significantly at 3 h (4.0-, 4.8- and 2.7-fold, respectively, P < 0.01), and reached the peak at 12 h (15.3- and 27.6-fold for LPS and β-glucan respectively, P < 0.01) and 24 h (17.3-fold for PGN, P < 0.01). In addition, TNF-α also induced the expression of CfNOS, which started to increase at 1 h (5.2-fold, P < 0.05) and peaked at 6 h (19.9-fold, P < 0.01). The catalytic activity of the native CfNOS protein was 30.3 ± 0.3 U mgprot-1, and it decreased significantly after the addition of the selective inhibitors of nNOS and iNOS (26.9 ± 0.4 and 29.3 ± 0.1 U mgprot-1, respectively, P < 0.01). Conclusions These results suggested that CfNOS, with identical structure with nNOS and similar enzymatic characteristics to nNOS and iNOS, played the immunological role of iNOS to be involved in the scallop immune defense against PAMPs and TNF-α. PMID:23922688

  9. 43 CFR 4.1362 - Where to file; when to file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Where to file; when to file. 4.1362... file; when to file. (a) The request for review shall be filed with the Hearings Division, Office of... bear the expense for this service. (b) Failure to file a request for review within the time...

  10. The Relationship Between Endothelial Nitric Oxide Synthase Gene (NOS3) Polymorphisms, NOS3 Expression, and Varicocele.

    PubMed

    Kahraman, Cigdem Yuce; Tasdemir, Sener; Sahin, Ibrahim; Marzioglu Ozdemir, Ebru; Yaralı, Oguzhan; Ziypak, Tevfik; Adanur, Senol; Kahraman, Mustafa; Tatar, Abdulgani

    2016-04-01

    Varicocele is an abnormal enlargement of the pampiniform venous plexus in the scrotum. Varicocele is the most common cause of secondary male infertility. Nitric oxide (NO), which has a role on varicocele pathophysiology, is synthesized by endothelial nitric oxide synthase gene (NOS3). In our study, we aimed to explain the relationship between varicocele, three common NOS3 polymorphisms (T-786C, G894T, 4b/a), and NOS3 mRNA expression levels. We investigated NOS3 T-786C, G894T, and 4b/a polymorphisms in 102 patients with varicocele and 100 healthy controls. Twenty-four patients and 17 controls were chosen for expression studies based on polymorphism subgroupings. Subgroup 1 includes patients who have no minor allele polymorphisms, and subgroups 2, 3, and 4 have T-786C, G894T, and 4b/a polymorphisms, respectively. The 4b/a polymorphism demonstrated significantly elevated levels in both allele and genotype analysis in the control group compared to the patient group. The G894T polymorphism was statistically elevated for genotypic frequencies in the patient group compared to the control group, but this finding did not extend to allelic frequencies. There were no statistically significant differences in either the allelic or genotypic frequencies between patients and control groups for the T-786C polymorphism. When patient and control expression levels were compared without considering the subgroups, the NOS3 expression level was found to be statistically higher in the patient group. There were no statistically significant differences in the patient and control group expression levels when stratified by subgroup, nor was there any effect of the polymorphisms under study on expression levels. The 4b/a polymorphism may have a protective effect for varicocelem and G894T polymorphism may contribute to varicocele occurrence by lowering the level of NO. The higher NOS3 expression levels in the patient group may be a kind of dilator compensatory mechanism to protect vascular

  11. Large File System Backup: NERSC Global File System Experience

    SciTech Connect

    Mokhtarani, Akbar; Andrews, Matthew; Hick, Jason; Kramer, William

    2008-10-23

    NERSC's Global File system (NGF), accessible from all compute systems at NERSC, holds files and data from many scientific projects. A full backup of this file system to our High Performance Storage System (HPSS) is performed periodically. Disk storage usage by projects at NERSC has grown seven fold over a two year period, from ~;;20TB in June 2006 to ~;;140 TB in June 2008. The latest full backup took about 13 days and more than 200 T10k tape cartridges (.5 TB capacity). Petabyte file systems are becoming a reality in the next few years and the existing utilities are already strained in handling backup tasks.

  12. MERRA-2: File Specification

    NASA Technical Reports Server (NTRS)

    Bosilovich, M. G.; Lucchesi, R.; Suarez, M.

    2015-01-01

    The second Modern-Era Retrospective analysis for Research and Applications (MERRA-2) is a NASA atmospheric reanalysis that begins in 1980. It replaces the original MERRA reanalysis (Rienecker et al., 2011) using an upgraded version of the Goddard Earth Observing System Model, Version 5 (GEOS-5) data assimilation system. The file collections for MERRA-2 are described in detail in this document, including some important changes from those of the MERRA dataset (Lucchesi, 2012).

  13. Secure Distributed File Systems

    DTIC Science & Technology

    1994-09-01

    practicality and applicability of one such system, Trusted Ficus File System ( TRUFFLES ), in the DoD infrastructure. Integrated into this research are...discussions of Privacy Enhanced Mail (PEM), which is currently an integral part of the TRUFFLES implementation. This thesis concludes with a discussion of the...actual installation of a PEM reference implementation, and future requirements for the TRUFFLES installation at the Naval Postgraduate School. 14

  14. The Galley Parallel File System

    NASA Technical Reports Server (NTRS)

    Nieuwejaar, Nils; Kotz, David

    1996-01-01

    As the I/O needs of parallel scientific applications increase, file systems for multiprocessors are being designed to provide applications with parallel access to multiple disks. Many parallel file systems present applications with a conventional Unix-like interface that allows the application to access multiple disks transparently. The interface conceals the parallelism within the file system, which increases the ease of programmability, but makes it difficult or impossible for sophisticated programmers and libraries to use knowledge about their I/O needs to exploit that parallelism. Furthermore, most current parallel file systems are optimized for a different workload than they are being asked to support. We introduce Galley, a new parallel file system that is intended to efficiently support realistic parallel workloads. We discuss Galley's file structure and application interface, as well as an application that has been implemented using that interface.

  15. The Galley Parallel File System

    NASA Technical Reports Server (NTRS)

    Nieuwejaar, Nils; Kotz, David

    1996-01-01

    Most current multiprocessor file systems are designed to use multiple disks in parallel, using the high aggregate bandwidth to meet the growing I/0 requirements of parallel scientific applications. Many multiprocessor file systems provide applications with a conventional Unix-like interface, allowing the application to access multiple disks transparently. This interface conceals the parallelism within the file system, increasing the ease of programmability, but making it difficult or impossible for sophisticated programmers and libraries to use knowledge about their I/O needs to exploit that parallelism. In addition to providing an insufficient interface, most current multiprocessor file systems are optimized for a different workload than they are being asked to support. We introduce Galley, a new parallel file system that is intended to efficiently support realistic scientific multiprocessor workloads. We discuss Galley's file structure and application interface, as well as the performance advantages offered by that interface.

  16. Ageing single file motion

    NASA Astrophysics Data System (ADS)

    Metzler, R.; Sanders, L.; Lomholt, M. A.; Lizana, L.; Fogelmark, K.; Ambjörnsson, Tobias

    2014-12-01

    The mean squared displacement of a tracer particle in a single file of identical particles with excluded volume interactions shows the famed Harris scaling ≃ K1/2t1/2 as function of time. Here we study what happens to this law when each particle of the single file interacts with the environment such that it is transiently immobilised for times τ with a power-law distribution ψ(τ) ≃ (τ★)α, and different ranges of the exponent α are considered. We find a dramatic slow-down of the motion of a tracer particle from Harris' law to an ultraslow, logarithmic time evolution ≃ K0 log 1/2(t) when 0 < α < 1. In the intermediate case 1 < α < 2, we observe a power-law form for the mean squared displacement, with a modified scaling exponent as compared to Harris' law. Once α is larger than two, the Brownian single file behaviour and thus Harris' law are restored. We also point out that this process is weakly non-ergodic in the sense that the time and ensemble averaged mean squared displacements are disparate.

  17. San Francisco Bay Area Endangered Species Litigation: The Complaint

    EPA Pesticide Factsheets

    The Center for Biological Diversity filed a lawsuit in the United States District Court for the Northern District of California alleging that EPA failed to comply with the ESA related to 47 pesticides and 11 endangered or threatened species.

  18. Reactive oxygen species in iridium-based OER catalysts† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc01860b Click here for additional data file.

    PubMed Central

    Pfeifer, Verena; Wrabetz, Sabine; Massué, Cyriac; Velasco Vélez, Juan J.; Arrigo, Rosa; Scherzer, Michael; Piccinin, Simone; Hävecker, Michael; Knop-Gericke, Axel; Schlögl, Robert

    2016-01-01

    Tremendous effort has been devoted towards elucidating the fundamental reasons for the higher activity of hydrated amorphous IrIII/IV oxyhydroxides (IrOx) in the oxygen evolution reaction (OER) in comparison with their crystalline counterpart, rutile-type IrO2, by focusing on the metal oxidation state. Here we demonstrate that, through an analogy to photosystem II, the nature of this reactive species is not solely a property of the metal but is intimately tied to the electronic structure of oxygen. We use a combination of synchrotron-based X-ray photoemission and absorption spectroscopies, ab initio calculations, and microcalorimetry to show that holes in the O 2p states in amorphous IrOx give rise to a weakly bound oxygen that is extremely susceptible to nucleophilic attack, reacting stoichiometrically with CO already at room temperature. As such, we expect this species to play the critical role of the electrophilic oxygen involved in O–O bond formation in the electrocatalytic OER on IrOx. We propose that the dynamic nature of the Ir framework in amorphous IrOx imparts the flexibility in Ir oxidation state required for the formation of this active electrophilic oxygen. PMID:28042464

  19. Regulation of eNOS-derived superoxide by endogenous methylarginines.

    PubMed

    Druhan, Lawrence J; Forbes, Scott P; Pope, Arthur J; Chen, Chun-An; Zweier, Jay L; Cardounel, Arturo J

    2008-07-08

    The endogenous methylarginines, asymmetric dimethylarginine (ADMA) and N (G)-monomethyl- l-arginine (L-NMMA) regulate nitric oxide (NO) production from endothelial NO synthase (eNOS). Under conditions of tetrahydrobiopterin (BH 4) depletion eNOS also generates (*)O 2 (-); however, the effects of methylarginines on eNOS-derived (*)O 2 (-) generation are poorly understood. Therefore, using electron paramagnetic resonance spin trapping techniques we measured the dose-dependent effects of ADMA and L-NMMA on (*)O 2 (-) production from eNOS under conditions of BH 4 depletion. In the absence of BH 4, ADMA dose-dependently increased NOS-derived (*)O 2 (-) generation, with a maximal increase of 151% at 100 microM ADMA. L-NMMA also dose-dependently increased NOS-derived (*)O 2 (-), but to a lesser extent, demonstrating a 102% increase at 100 microM L-NMMA. Moreover, the native substrate l-arginine also increased eNOS-derived (*)O 2 (-), exhibiting a similar degree of enhancement as that observed with ADMA. Measurements of NADPH consumption from eNOS demonstrated that binding of either l-arginine or methylarginines increased the rate of NADPH oxidation. Spectrophotometric studies suggest, just as for l-arginine and L-NMMA, the binding of ADMA shifts the eNOS heme to the high-spin state, indicative of a more positive heme redox potential, enabling enhanced electron transfer from the reductase to the oxygenase site. These results demonstrate that the methylarginines can profoundly shift the balance of NO and (*)O 2 (-) generation from eNOS. These observations have important implications with regard to the therapeutic use of l-arginine and the methylarginine-NOS inhibitors in the treatment of disease.

  20. 77 FR 35370 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-13

    .... Description: El Paso Natural Gas Company's 2011 (Jan-Mar) Penalty Credit Report. Filed Date: 5/31/12... following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket...

  1. 18 CFR 34.7 - Filing requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... SECURITIES OR THE ASSUMPTION OF LIABILITIES § 34.7 Filing requirements. Applications must be filed with the Secretary of the Commission in accordance with filing procedures posted on the Commission's Web site at http...

  2. 18 CFR 34.7 - Filing requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... SECURITIES OR THE ASSUMPTION OF LIABILITIES § 34.7 Filing requirements. Applications must be filed with the Secretary of the Commission in accordance with filing procedures posted on the Commission's Web site at...

  3. 76 FR 48836 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... Line Company, LLC submits tariff filing per 154.403: S-2 Tracker Filing to be effective 8/1/2011. Filed...-000. Applicants: Cimarron River Pipeline, LLC. Description: 2011 Annual Report of Cash Out Activity...

  4. 78 FR 55694 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-11

    ....ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free...--September 2013--Tenaska Attachment A to be effective 12/31/9998. Filed Date: 9/4/13. Accession Number...

  5. Cytoscape file of chemical networks

    EPA Pesticide Factsheets

    The maximum connectivity scores of pairwise chemical conditions summarized from Cmap results in a file with Cytoscape format (http://www.cytoscape.org/). The figures in the publication were generated from this file. The Cytoscape file is formed from importing the eight text file therein.This dataset is associated with the following publication:Wang , R., A. Biales , N. Garcia-Reyero, E. Perkins, D. Villeneuve, G. Ankley, and D. Bencic. Fish Connectivity Mapping: Linking Chemical Stressors by Their MOA-Driven Transcriptomic Profiles. BMC Genomics. BioMed Central Ltd, London, UK, 17(84): 1-20, (2016).

  6. The Internet Toolkit: File Compression and Archive Utilities.

    ERIC Educational Resources Information Center

    Delfino, Erik

    1993-01-01

    Describes utility programs available for posttransfer processing files that have been downloaded from the Internet. Highlights include file compression; viewing graphics files; converting binary files to ASCII files; and how to find utility programs. (LRW)

  7. Face and Emotion Recognition in MCDD versus PDD-NOS

    ERIC Educational Resources Information Center

    Herba, Catherine M.; de Bruin, Esther; Althaus, Monika; Verheij, Fop; Ferdinand, Robert F.

    2008-01-01

    Previous studies indicate that Multiple Complex Developmental Disorder (MCDD) children differ from PDD-NOS and autistic children on a symptom level and on psychophysiological functioning. Children with MCDD (n = 21) and PDD-NOS (n = 62) were compared on two facets of social-cognitive functioning: identification of neutral faces and facial…

  8. Face and Emotion Recognition in MCDD versus PDD-NOS

    ERIC Educational Resources Information Center

    Herba, Catherine M.; de Bruin, Esther; Althaus, Monika; Verheij, Fop; Ferdinand, Robert F.

    2008-01-01

    Previous studies indicate that Multiple Complex Developmental Disorder (MCDD) children differ from PDD-NOS and autistic children on a symptom level and on psychophysiological functioning. Children with MCDD (n = 21) and PDD-NOS (n = 62) were compared on two facets of social-cognitive functioning: identification of neutral faces and facial…

  9. 15 CFR Supplement Nos. 2-3 to Part... - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Nos. Supplement Nos. 2-3 to Part 716 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS INITIAL AND ROUTINE...

  10. 15 CFR Supplement Nos. 2-3 to Part... - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Nos. Supplement Nos. 2-3 to Part 716 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS INITIAL AND ROUTINE...

  11. 15 CFR Supplement Nos. 2-3 to Part... - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Nos. Supplement Nos. 2-3 to Part 716 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS INITIAL AND ROUTINE...

  12. 15 CFR Supplement Nos. 2-3 to Part... - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Nos. Supplement Nos. 2-3 to Part 716 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS INITIAL AND ROUTINE...

  13. 15 CFR Supplement Nos. 2-3 to Part... - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Nos. Supplement Nos. 2-3 to Part 716 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS INITIAL AND ROUTINE...

  14. Converting Inhouse Subject Card Files to Electronic Keyword Files.

    ERIC Educational Resources Information Center

    Culmer, Carita M.

    The library at Phoenix College developed the Controversial Issues Files (CIF), a "home made" card file containing references pertinent to specific ongoing assignments. Although the CIF had proven itself to be an excellent resource tool for beginning researchers, it was cumbersome to maintain in the card format, and was limited to very…

  15. 76 FR 53676 - Combined Notice of Filings; Filings Instituting Proceedings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-29

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings; Filings Instituting Proceedings Take... Northern's FERC Gas Tariff to resolve a prior period imbalance with the high Monthly Index Price...

  16. Converting Inhouse Subject Card Files to Electronic Keyword Files.

    ERIC Educational Resources Information Center

    Culmer, Carita M.

    The library at Phoenix College developed the Controversial Issues Files (CIF), a "home made" card file containing references pertinent to specific ongoing assignments. Although the CIF had proven itself to be an excellent resource tool for beginning researchers, it was cumbersome to maintain in the card format, and was limited to very…

  17. IFN-γ regulates xanthine oxidase-mediated iNOS-independent oxidative stress in maneb- and paraquat-treated rat polymorphonuclear leukocytes.

    PubMed

    Singh, Deepali; Kumar, Vinod; Singh, Chetna

    2017-03-01

    Maneb (MB) and paraquat (PQ) provoke oxidative stress-mediated cell damage. Role of xanthine oxidase (XO) in oxidative stress and its association with nitric oxide (NO)/NO synthase (NOS) have been widely reported. While inducible NOS (iNOS) is implicated in MB+PQ-induced toxicity in rat polymorphonuclear leukocytes (PMNs), role of XO and its alliance with iNOS have not yet been established. The study investigated the role of XO in MB+PQ-induced oxidative stress in rat PMNs and its regulation by iNOS and inflammatory cytokines. MB+PQ-augmented reactive oxygen species (ROS), superoxide, nitro-tyrosine, lipid peroxidation (LPO), and nitrite levels along with the catalytic activity of iNOS, superoxide dismutase (SOD), and XO. XO inhibitor, allopurinol (AP), alleviated MB+PQ-induced changes except nitrite content and iNOS activity. Conversely, an iNOS inhibitor, aminoguanidine, mitigated MB+PQ-induced LPO, nitrite, iNOS, and nitro-tyrosine levels; however, no change was observed in ROS, SOD, and XO. Nuclear factor-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), tumor necrosis factor-alpha (TNF-α) inhibitor, pentoxyfylline, and an anti-inflammatory agent, dexamethasone, attenuated MB+PQ-induced increase in XO, superoxide, and ROS with parallel reduction in the expression of interferon-gamma (IFN-γ), TNF-α, and interleukin-1β (IL-1β) in rat PMNs. Exogenous IFN-γ, TNF-α, and IL-1β enhanced superoxide, ROS, and XO in the PMNs of control and MB+PQ-treated rats; however, IFN- γ was found to be the most potent inducer. Moreover, AP ameliorated cytokine-induced free radical generation and restored XO activity towards normalcy. The results thus demonstrate that XO mediates oxidative stress in MB+PQ-treated rat PMNs via iNOS-independent but cytokine (predominantly IFN-γ)-dependent mechanism.

  18. Storing files in a parallel computing system using list-based index to identify replica files

    DOEpatents

    Faibish, Sorin; Bent, John M.; Tzelnic, Percy; Zhang, Zhenhua; Grider, Gary

    2015-07-21

    Improved techniques are provided for storing files in a parallel computing system using a list-based index to identify file replicas. A file and at least one replica of the file are stored in one or more storage nodes of the parallel computing system. An index for the file comprises at least one list comprising a pointer to a storage location of the file and a storage location of the at least one replica of the file. The file comprises one or more of a complete file and one or more sub-files. The index may also comprise a checksum value for one or more of the file and the replica(s) of the file. The checksum value can be evaluated to validate the file and/or the file replica(s). A query can be processed using the list.

  19. Endothelial function does not improve with high-intensity continuous exercise training in SHR: implications of eNOS uncoupling.

    PubMed

    Battault, Sylvain; Singh, François; Gayrard, Sandrine; Zoll, Joffrey; Reboul, Cyril; Meyer, Grégory

    2016-02-01

    Exercise training is a well-recognized way to improve vascular endothelial function by increasing nitric oxide (NO) bioavailability. However, in hypertensive subjects, unlike low- and moderate-intensity exercise training, the beneficial effects of continuous high-intensity exercise on endothelial function are not clear, and the underlying mechanisms remain unknown. The aim of this study was to investigate the impact of high-intensity exercise on vascular function, especially on the NO pathway, in spontaneous hypertensive rats (SHR). These effects were studied on WKY, sedentary SHR and SHR that exercised at moderate (SHR-MOD) and high intensity (SHR-HI) on a treadmill (1 h per day; 5 days per week for 6 weeks at 55% and 80% of their maximal aerobic velocity, respectively). Endothelial function and specific NO contributions to acetylcholine-mediated relaxation were evaluated by measuring the aortic ring isometric forces. Endothelial nitric oxide synthase (eNOS) expression and phosphorylation (ser1177) were evaluated by western blotting. The total aortic and eNOS-dependent reactive oxygen species (ROS) production was assessed using electron paramagnetic resonance in aortic tissue. Although the aortas of SHR-HI had increased eNOS levels without alteration of eNOS phosphorylation, high-intensity exercise had no beneficial effect on endothelium-dependent vasorelaxation, unlike moderate exercise. This result was associated with increased eNOS-dependent ROS production in the aortas of SHR-HI. Notably, the use of the recoupling agent BH4 or a thiol-reducing agent blunted eNOS-dependent ROS production in the aortas of SHR-HI. In conclusion, the lack of a positive effect of high-intensity exercise on endothelial function in SHR was mainly explained by redox-dependent eNOS uncoupling, resulting in a switch from NO to O2(-) generation.

  20. DISTRIBUTION OF NOS ISOFORMS IN A PORCINE ENDOTOXIN SHOCK MODEL

    PubMed Central

    Doursout, Marie-Francoise; Oguchi, Takeshi; Fischer, Uwe M.; Liang, YangYan; Chelly, Brice; Hartley, Craig J.; Chelly, Jacques E.

    2012-01-01

    Sepsis is a major cause of morbidity and mortality. NO, an endogenous vasodilator, has been associated with the hypotension, catecholamine hyporesponsiveness, and myocardial depression of septic shock. Although iNOS is thought to be responsible for the hypotension and loss of vascular tone occurring several hours after endotoxin administration, little is known on the effects of constitutive eNOS on LPS-induced organ dysfunction. This study assessed the distribution of eNOS and iNOS in various vascular beds in conscious pigs challenged with LPS. Cardiac and regional hemodynamic parameters were recorded over 8 h in the presence and absence of aminoguanidine, a rather selective inhibitor of iNOS activity, and N-methyl-L-arginine, a nonspecific NOS inhibitor. Our data show that LPS-induced cardiac depression was associated with coronary, renal, and mesenteric vasoconstrictions and a hepatic vasodilatation. LPS also induced increases in eNOS in the heart and lungs, whereas iNOS was mostly detected in the liver. Nitrotyrosine formation was mainly detected in the lungs, with traces in the kidney, liver, and gut. Accordingly, our results suggest that the early decrease in blood pressure and cardiac depression are likely due to activated eNOS, whereas both isoforms are involved in the hepatic vasodilation. In contrast, carotid, coronary, mesenteric, and renal vasoconstrictions were significant at 5 and/or 6 h after LPS infusion, suggesting that NO is not the primary mediator, facilitating and/or unmasking the release of vasoconstrictor mediators. Consequently, developing newer tissue- or isoform-specific NOS inhibitors can lead to novel therapeutic agents in septic shock. PMID:17909454

  1. Vascular nitric oxide: Beyond eNOS.

    PubMed

    Zhao, Yingzi; Vanhoutte, Paul M; Leung, Susan W S

    2015-10-01

    As the first discovered gaseous signaling molecule, nitric oxide (NO) affects a number of cellular processes, including those involving vascular cells. This brief review summarizes the contribution of NO to the regulation of vascular tone and its sources in the blood vessel wall. NO regulates the degree of contraction of vascular smooth muscle cells mainly by stimulating soluble guanylyl cyclase (sGC) to produce cyclic guanosine monophosphate (cGMP), although cGMP-independent signaling [S-nitrosylation of target proteins, activation of sarco/endoplasmic reticulum calcium ATPase (SERCA) or production of cyclic inosine monophosphate (cIMP)] also can be involved. In the blood vessel wall, NO is produced mainly from l-arginine by the enzyme endothelial nitric oxide synthase (eNOS) but it can also be released non-enzymatically from S-nitrosothiols or from nitrate/nitrite. Dysfunction in the production and/or the bioavailability of NO characterizes endothelial dysfunction, which is associated with cardiovascular diseases such as hypertension and atherosclerosis.

  2. Advancing the Perceptions of the Nature of Science (NOS): Integrating Teaching the NOS in a Science Content Course

    ERIC Educational Resources Information Center

    Aflalo, Ester

    2014-01-01

    Background: Understanding the nature of science (NOS) has been a key objective in teaching sciences for many years. Despite the importance of this goal it is, until this day, a complex challenge that we are far from achieving. Purpose: The study was conducted in order to further the understanding of the NOS amongst preservice teachers. It explores…

  3. Advancing the Perceptions of the Nature of Science (NOS): Integrating Teaching the NOS in a Science Content Course

    ERIC Educational Resources Information Center

    Aflalo, Ester

    2014-01-01

    Background: Understanding the nature of science (NOS) has been a key objective in teaching sciences for many years. Despite the importance of this goal it is, until this day, a complex challenge that we are far from achieving. Purpose: The study was conducted in order to further the understanding of the NOS amongst preservice teachers. It explores…

  4. The School Library Vertical File.

    ERIC Educational Resources Information Center

    Smallwood, Carol

    1990-01-01

    Discusses the maintenance of vertical files in the school library. Topics covered include circulation, weeding, using materials for special displays, acquiring materials, policies on advertising and controversial issues, cross-references, subject headings, introducing students to vertical files, beginning a collection, and preservation. (MES)

  5. Software for Managing Personal Files.

    ERIC Educational Resources Information Center

    Lundeen, Gerald

    1989-01-01

    Discusses the special characteristics of personal file management software and compares four microcomputer software packages: Notebook II with Bibliography and Convert, Pro-Cite with Biblio-Links, askSam, and Reference Manager. Each package is evaluated in terms of the user interface, file maintenance, retrieval capabilities, output, and…

  6. Portable File Format (PFF) specifications

    SciTech Connect

    Dolan, Daniel H.

    2015-02-01

    Created at Sandia National Laboratories, the Portable File Format (PFF) allows binary data transfer across computer platforms. Although this capability is supported by many other formats, PFF files are still in use at Sandia, particularly in pulsed power research. This report provides detailed PFF specifications for accessing data without relying on legacy code.

  7. The flavinyl transferase ApbE of Pseudomonas stutzeri matures the NosR protein required for nitrous oxide reduction.

    PubMed

    Zhang, Lin; Trncik, Christian; Andrade, Susana L A; Einsle, Oliver

    2017-02-01

    The copper-containing enzyme nitrous oxide reductase (N2OR) catalyzes the transformation of nitrous oxide (N2O) to dinitrogen (N2) in microbial denitrification. Several accessory factors are essential for assembling the two copper sites CuA and CuZ, and for maintaining the activity. In particular, the deletion of either the transmembrane iron-sulfur flavoprotein NosR or the periplasmic protein NosX, a member of the ApbE family, abolishes N2O respiration. Here we demonstrate through biochemical and structural studies that the ApbE protein from Pseudomonas stutzeri, where the nosX gene is absent, is a monomeric FAD-binding protein that can serve as the flavin donor for NosR maturation via covalent flavinylation of a threonine residue. The flavin transfer reaction proceeds both in vivo and in vitro to generate post-translationally modified NosR with covalently bound FMN. Only FAD can act as substrate and the reaction requires a divalent cation, preferably Mg(2+) that was also present in the crystal structure. In addition, the reaction is species-specific to a certain extent.

  8. Endothelial NOS-dependent activation of c-Jun NH(2)- terminal kinase by oxidized low-density lipoprotein

    NASA Technical Reports Server (NTRS)

    Go, Y. M.; Levonen, A. L.; Moellering, D.; Ramachandran, A.; Patel, R. P.; Jo, H.; Darley-Usmar, V. M.

    2001-01-01

    Oxidized low-density lipoprotein (oxLDL) is known to activate a number of signal transduction pathways in endothelial cells. Among these are the c-Jun NH(2)-terminal kinase (JNK), also known as stress-activated protein kinase, and extracellular signal-regulated kinase (ERK). These mitogen-activated protein kinases (MAP kinase) determine cell survival in response to environmental stress. Interestingly, JNK signaling involves redox-sensitive mechanisms and is activated by reactive oxygen and nitrogen species derived from both NADPH oxidases, nitric oxide synthases (NOS), peroxides, and oxidized low-density lipoprotein (oxLDL). The role of endothelial NOS (eNOS) in the activation of JNK in response to oxLDL has not been examined. Herein, we show that on exposure of endothelial cells to oxLDL, both ERK and JNK are activated through independent signal transduction pathways. A key role of eNOS activation through a phosphatidylinositol-3-kinase-dependent mechanism leading to phosphorylation of eNOS is demonstrated for oxLDL-dependent activation of JNK. Moreover, we show that activation of ERK by oxLDL is critical in protection against the cytotoxicity of oxLDL.

  9. Endothelial NOS-dependent activation of c-Jun NH(2)- terminal kinase by oxidized low-density lipoprotein

    NASA Technical Reports Server (NTRS)

    Go, Y. M.; Levonen, A. L.; Moellering, D.; Ramachandran, A.; Patel, R. P.; Jo, H.; Darley-Usmar, V. M.

    2001-01-01

    Oxidized low-density lipoprotein (oxLDL) is known to activate a number of signal transduction pathways in endothelial cells. Among these are the c-Jun NH(2)-terminal kinase (JNK), also known as stress-activated protein kinase, and extracellular signal-regulated kinase (ERK). These mitogen-activated protein kinases (MAP kinase) determine cell survival in response to environmental stress. Interestingly, JNK signaling involves redox-sensitive mechanisms and is activated by reactive oxygen and nitrogen species derived from both NADPH oxidases, nitric oxide synthases (NOS), peroxides, and oxidized low-density lipoprotein (oxLDL). The role of endothelial NOS (eNOS) in the activation of JNK in response to oxLDL has not been examined. Herein, we show that on exposure of endothelial cells to oxLDL, both ERK and JNK are activated through independent signal transduction pathways. A key role of eNOS activation through a phosphatidylinositol-3-kinase-dependent mechanism leading to phosphorylation of eNOS is demonstrated for oxLDL-dependent activation of JNK. Moreover, we show that activation of ERK by oxLDL is critical in protection against the cytotoxicity of oxLDL.

  10. Efficient algorithms for multi-file caching

    SciTech Connect

    Otoo, Ekow J.; Rotem, Doron; Seshadri, Sridhar

    2004-03-15

    Multi-File Caching issues arise in applications where a set of jobs are processed and each job requests one or more input files. A given job can only be started if all its input files are preloaded into a disk cache. Examples of applications where Multi-File caching may be required are scientific data mining, bit-sliced indexes, and analysis of sets of vertically partitioned files. The difference between this type of caching and traditional file caching systems is that in this environment, caching and replacement decisions are made based on ''combinations of files (file bundles),'' rather than single files. In this work we propose new algorithms for Multi-File caching and analyze their performance. Extensive simulations are presented to establish the effectiveness of the Multi-File caching algorithm in terms of job response time and job queue length.

  11. The DNA Files

    SciTech Connect

    1998-06-09

    The DNA Files is a radio documentary which disseminates genetics information over public radio. The documentaries explore subjects which include the following: How genetics affects society. How human life began and how it evolved. Could new prenatal genetic tests hold the key to disease prevention later in life? Would a national genetic data base sacrifice individual privacy? and Should genes that may lead to the cure for cancer be privately owned? This report serves as a project update for the second quarter of 1998. It includes the spring/summer 1998 newsletter, the winter 1998 newsletter, the program clock, and the latest flyer.

  12. Species Conservation and Management: Case Studies

    USGS Publications Warehouse

    Akcakaya, H.R.; Burgman, M.A.; Kindvall, O.; Wood, C.C.; Sjogren-Gulve, P.; Hatfield, J.S.; McCarthy, M.A.

    2004-01-01

    This edited volume is a collection of population and metapopulation models for a wide variety of species, including plants, invertebrates, fishes, amphibians, reptiles, birds, and mammals. Each chapter of the book describes the application of RAMAS GIS 4.0 to one species, with the aim of demonstrating how various life history characteristics of the species are incorporated into the model, and how the results of the model has been or can be used in conservation and management of the species. The book comes with a CD that includes a demo version of the program, and the data files for each species.

  13. Using a Professional Development Program for Enhancing Chilean Biology Teachers' Understanding of Nature of Science (NOS) and Their Perceptions About Using History of Science to Teach NOS

    NASA Astrophysics Data System (ADS)

    Pavez, José M.; Vergara, Claudia A.; Santibañez, David; Cofré, Hernán

    2016-05-01

    A number of authors have recognized the importance of understanding the nature of science (NOS) for scientific literacy. Different instructional strategies such as decontextualized, hands-on inquiry, and history of science (HOS) activities have been proposed for teaching NOS. This article seeks to understand the contribution of HOS in enhancing biology teachers' understanding of NOS, and their perceptions about using HOS to teach NOS. These teachers ( N = 8), enrolled in a professional development program in Chile are, according to the national curriculum, expected to teach NOS, but have no specific NOS and HOS training. Teachers' views of NOS were assessed using the VNOS-D+ questionnaire at the beginning and at the end of two modules about science instruction and NOS. Both the pre- and the post-test were accompanied by interviews, and in the second session we collected information about teachers' perceptions of which interventions had been more significant in changing their views on NOS. Finally, the teachers also had to prepare a lesson plan for teaching NOS that included HOS. Some of the most important study results were: significant improvements were observed in teachers' understanding of NOS, although they assigned different levels of importance to HOS in these improvements; and although the teachers improved their understanding of NOS, most had difficulties in planning lessons about NOS and articulating historical episodes that incorporated NOS. The relationship between teachers' improved understanding of NOS and their instructional NOS skills is also discussed.

  14. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... to be filed under 11 CFR parts 101, 102, 104, 105, 107, 108, and 109, and any modifications or...) Where to deliver reports. Except for documents electronically filed under paragraph (c) of this section...., Washington, DC 20463; or the Secretary of the United States Senate, Office of Public Records, 119 D Street...

  15. NOS-2 Inhibition in Phosgene-Induced Acute Lung Injury.

    PubMed

    Filipczak, Piotr T; Senft, Albert P; Seagrave, JeanClare; Weber, Waylon; Kuehl, Philip J; Fredenburgh, Laura E; McDonald, Jacob D; Baron, Rebecca M

    2015-07-01

    Phosgene exposure via an industrial or warfare release produces severe acute lung injury (ALI) with high mortality, characterized by massive pulmonary edema, disruption of epithelial tight junctions, surfactant dysfunction, and oxidative stress. There are no targeted treatments for phosgene-induced ALI. Previous studies demonstrated that nitric oxide synthase 2 (NOS-2) is upregulated in the lungs after phosgene exposure; however, the role of NOS-2 in the pathogenesis of phosgene-induced ALI remains unknown. We previously demonstrated that NOS-2 expression in lung epithelium exacerbates inhaled endotoxin-induced ALI in mice, mediated partially through downregulation of surfactant protein B (SP-B) expression. Therefore, we hypothesized that a selective NOS-2 inhibitor delivered to the lung epithelium by inhalation would mitigate phosgene-induced ALI. Inhaled phosgene produced increases in bronchoalveolar lavage fluid protein, histologic lung injury, and lung NOS-2 expression at 24 h. Administration of the selective NOS-2 inhibitor 1400 W via inhalation, but not via systemic delivery, significantly attenuated phosgene-induced ALI and preserved epithelial barrier integrity. Furthermore, aerosolized 1400 W augmented expression of SP-B and prevented downregulation of tight junction protein zonula occludens 1 (ZO-1), both critical for maintenance of normal lung physiology and barrier integrity. We also demonstrate for the first time that NOS-2-derived nitric oxide downregulates the ZO-1 expression at the transcriptional level in human lung epithelial cells, providing a novel target for ameliorating vascular leak in ALI. Our data demonstrate that lung NOS-2 plays a critical role in the development of phosgene-induced ALI and suggest that aerosolized NOS-2 inhibitors offer a novel therapeutic strategy for its treatment. © The Author 2015. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please e

  16. NOS-2 Inhibition in Phosgene-Induced Acute Lung Injury

    PubMed Central

    Filipczak, Piotr T.; Senft, Albert P.; Seagrave, JeanClare; Weber, Waylon; Kuehl, Philip J.; Fredenburgh, Laura E.; McDonald, Jacob D.; Baron, Rebecca M.

    2015-01-01

    Phosgene exposure via an industrial or warfare release produces severe acute lung injury (ALI) with high mortality, characterized by massive pulmonary edema, disruption of epithelial tight junctions, surfactant dysfunction, and oxidative stress. There are no targeted treatments for phosgene-induced ALI. Previous studies demonstrated that nitric oxide synthase 2 (NOS-2) is upregulated in the lungs after phosgene exposure; however, the role of NOS-2 in the pathogenesis of phosgene-induced ALI remains unknown. We previously demonstrated that NOS-2 expression in lung epithelium exacerbates inhaled endotoxin-induced ALI in mice, mediated partially through downregulation of surfactant protein B (SP-B) expression. Therefore, we hypothesized that a selective NOS-2 inhibitor delivered to the lung epithelium by inhalation would mitigate phosgene-induced ALI. Inhaled phosgene produced increases in bronchoalveolar lavage fluid protein, histologic lung injury, and lung NOS-2 expression at 24 h. Administration of the selective NOS-2 inhibitor 1400 W via inhalation, but not via systemic delivery, significantly attenuated phosgene-induced ALI and preserved epithelial barrier integrity. Furthermore, aerosolized 1400 W augmented expression of SP-B and prevented downregulation of tight junction protein zonula occludens 1 (ZO-1), both critical for maintenance of normal lung physiology and barrier integrity. We also demonstrate for the first time that NOS-2-derived nitric oxide downregulates the ZO-1 expression at the transcriptional level in human lung epithelial cells, providing a novel target for ameliorating vascular leak in ALI. Our data demonstrate that lung NOS-2 plays a critical role in the development of phosgene-induced ALI and suggest that aerosolized NOS-2 inhibitors offer a novel therapeutic strategy for its treatment. PMID:25870319

  17. 25 CFR 580.5 - What happens if I file late or fail to file?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false What happens if I file late or fail to file? 580.5 Section 580.5 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR APPEAL PROCEEDINGS... What happens if I file late or fail to file? (a) Failure to file an appeal within the time provided...

  18. Regulation of eNOS enzyme activity by posttranslational modification.

    PubMed

    Heiss, Elke H; Dirsch, Verena M

    2014-01-01

    The regulation of endothelial NO synthase (eNOS) employs multiple different cellular control mechanisms impinging on level and activity of the enzyme. This review aims at summarizing the current knowledge on the posttranslational modifications of eNOS, including acylation, nitrosylation, phosphorylation, acetylation, glycosylation and glutathionylation. Sites, mediators and impact on enzyme localization and activity of the single modifications will be discussed. Moreover, interdependence, cooperativity and competition between the different posttranslational modifications will be elaborated with special emphasis on the susceptibility of eNOS to metabolic cues.

  19. Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum.

    PubMed

    Velasco, Leonardo; Mesa, Socorro; Xu, Chang-Ai; Delgado, María J; Bedmar, Eulogio J

    2004-04-01

    The nosRZDFYLX gene cluster for the respiratory nitrous oxide reductase from Bradyrhizobium japonicum strain USDA110 has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid sequence exhibited a high degree of similarity to other nitrous oxide reductases from various sources. The NosZ protein included a signal peptide for protein export. Mutant strains carrying either a nosZ or a nosR mutation accumulated nitrous oxide when cultured microaerobically in the presence of nitrate. Maximal expression of a P nosZ-lacZ fusion in strain USDA110 required simultaneously both low level oxygen conditions and the presence of nitrate. Microaerobic activation of the fusion required FixLJ and FixK(2).

  20. Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle.

    PubMed

    Delgado, F; Estrada-Chávez, C; Romano, M; Paolicchi, F; Blanco-Viera, F; Capellino, F; Chavez-Gris, G; Pereira-Suárez, A L

    2010-09-01

    Paratuberculosis (PTB) is a chronic disease caused by M. avium subsp. paratuberculosis (MAP) that affects several animal species, and some studies have suggested that there may be a relationship between Crohn's disease and PTB. Significant aspects of PTB pathogenesis are not yet completely understood, such as the role of macrophages. Natural resistance-associated macrophage protein 1 (NRAMP1) and the inducible nitric oxide synthase (iNOS) molecules have shown nonspecific effects against several intracellular pathogens residing within macrophages. However, these molecules have been scarcely studied during natural infection with MAP. In this work, changes in NRAMP1 and iNOS expression were surveyed by immunohistochemistry in tissue samples from MAP-infected cattle and healthy controls. Our findings show strong specific immunolabeling against both NRAMP1 and iNOS molecules, throughout granulomatous PTB-compatible lesions in ileum and ileocaecal lymph nodes from paratuberculous cattle compared with uninfected controls, suggesting a relationship between the expression of these molecules and the pathogenesis of PTB disease. Copyright 2009 Elsevier Ltd. All rights reserved.

  1. Mitochondrial metabolic states and membrane potential modulate mtNOS activity.

    PubMed

    Valdez, Laura B; Zaobornyj, Tamara; Boveris, Alberto

    2006-03-01

    The mitochondrial metabolic state regulates the rate of NO release from coupled mitochondria: NO release by heart, liver and kidney mitochondria was about 40-45% lower in state 3 (1.2, 0.7 and 0.4 nmol/min mg protein) than in state 4 (2.2, 1.3 and 0.7 nmol/min mg protein). The activity of mtNOS, responsible for NO release, appears driven by the membrane potential component and not by intramitochondrial pH of the proton motive force. The intramitochondrial concentrations of the NOS substrates, L-arginine (about 310 microM) and NADPH (1.04-1.78 mM) are 60-1000 times higher than their KM values. Moreover, the changes in their concentrations in the state 4-state 3 transition are not enough to explain the changes in NO release. Nitric oxide release was exponentially dependent on membrane potential as reported for mitochondrial H2O2 production [S.S. Korshunov, V.P. Skulachev, A.A. Satarkov, High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria. FEBS Lett. 416 (1997) 15-18.]. Agents that decrease or abolish membrane potential minimize NO release while the addition of oligomycin that produces mitochondrial hyperpolarization generates the maximal NO release. The regulation of mtNOS activity, an apparently voltage-dependent enzyme, by membrane potential is marked at the physiological range of membrane potentials.

  2. Geranylgeranylacetone protects against cerebral ischemia and reperfusion injury: HSP90 and eNOS phosphorylation involved.

    PubMed

    He, Dake; Song, Xiaoqing; Li, Ling

    2015-03-02

    Cerebral ischemia and reperfusion (I/R) can trigger a cytotoxic cascade with overflow of reactive oxygen species, paradoxically causing neurological dysfunction, redox imbalance, inflammation and apoptosis. The present study aims to investigate the effect of geranylgeranylacetone(GGA) on cerebral I/R injury and the underlying mechanism. The results demonstrated that cerebral I/R increased the neurological function abnormality, brain edema, inflammation and oxidative injury in rats as well as the cognitive impairment, which was significantly reversed by GGA in a dose-dependent manner. GGA also suppressed the cell injury and apoptosis caused by cerebral I/R. Moreover, the protective effect of GGA was found to involve heat shock protein 90 (HSP90) and phosphorylated endothelial nitric oxide synthase (eNOS) expression and activity. Both the HSP90 and eNOS inhibitor abolished the effect of GGA. The data showed that GGA could protect rats against cerebral I/R injury, which may be related to the induction of HSP90 and activation of eNOS. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. 48 CFR 204.802 - Contract files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contract files. 204.802... OF DEFENSE GENERAL ADMINISTRATIVE MATTERS Contract Files 204.802 Contract files. Official contract files shall consist of— (1) Only original, authenticated or conformed copies of contractual...

  4. 10 CFR 603.1020 - File documents.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false File documents. 603.1020 Section 603.1020 Energy... Reporting Information About the Award § 603.1020 File documents. The award file should include an analysis..., the file must document the analysis required (see § 603.560) to set the fixed level of Federal...

  5. Tropospheric Emission Spectrometer Product File Readers

    NASA Technical Reports Server (NTRS)

    Fisher, Brendan M.

    2010-01-01

    TES Product File Reader software extracts data from publicly available Tropospheric Emission Spectrometer (TES) HDF (Hierarchical Data Format) product data files using publicly available format specifications for scientific analysis in IDL (interactive data language). In this innovation, the software returns data fields as simple arrays for a given file. A file name is provided, and the contents are returned as simple IDL variables.

  6. 18 CFR 1308.27 - Appeal files.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Appeal files. 1308.27... Board of Contract Appeals § 1308.27 Appeal files. (a) Notices of appeal shall be filed as provided in... an appeal file consisting of: (1) The Contracting Officer's decision, if any, from which the...

  7. 50 CFR 20.155 - Public file.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Public file. 20.155 Section 20.155... § 20.155 Public file. (a) Establishment. A public file will be established for each rulemaking to which.... 552, a public file established pursuant to paragraph (a) of this section will contain: (1) The...

  8. 47 CFR 1.1704 - Station files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Station files. 1.1704 Section 1.1704... System (COALS) § 1.1704 Station files. Applications, notifications, correspondence, electronic filings.... These files constitute the official records for these stations and supersede any other records,...

  9. 77 FR 56197 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-12

    ... received the following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket Numbers: RP11-2473-000. Applicants: Gulf South Pipeline Company, LP. Description: Annual Cash-In/Cash-Out Filing--2012. Filed Date: 8/31/12. Accession Number: 20120831-5044. Comments Due: 5 p.m. ET...

  10. 48 CFR 1404.802 - Contract files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract files. 1404.802 Section 1404.802 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL ADMINISTRATIVE MATTERS Contract Files 1404.802 Contract files. In addition to the requirements in FAR 4.802, files shall...

  11. 12 CFR 11.4 - Filing fees.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 1 2011-01-01 2011-01-01 false Filing fees. 11.4 Section 11.4 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY SECURITIES EXCHANGE ACT DISCLOSURE RULES § 11.4 Filing fees. (a) The OCC may require filing fees to accompany certain filings made under...

  12. 76 FR 1421 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ... filing per 154.203: Annual Flowthrough Crediting Mechanism to be effective N/ A. Filed Date: 12/30/2010... Transmission Company, LLC submits tariff filing per 154.203: Annual Accounting Report to be effective N/A.... Description: National Fuel Gas Supply Corporation submits tariff filing per 154.204: 2011 January IG Rate to...

  13. 76 FR 79169 - Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-21

    ... filing per 35.13(a)(2)(iii): Corn Belt Agreements to be effective 2/13/2012. Filed Date: 12/12/11....ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll...

  14. 12 CFR 622.18 - Filing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Formal Hearings § 622.18 Filing. (a) Filing. Papers required or permitted to be filed with the Board shall be filed with the Chairman of the Board, FCA, 1501 Farm Credit Drive, McLean, VA 22102-5090 or... testimony and exhibits), unless otherwise specifically provided by the Board. ...

  15. 12 CFR 1102.24 - Filing requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 9 2012-01-01 2012-01-01 false Filing requirements. 1102.24 Section 1102.24 Banks and Banking FEDERAL FINANCIAL INSTITUTIONS EXAMINATION COUNCIL APPRAISER REGULATION Rules of Practice for Proceedings § 1102.24 Filing requirements. (a) Filing. All papers filed with the ASC in...

  16. 12 CFR 303.121 - Filing procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Filing procedures. 303.121 Section 303.121 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES OF PRACTICE FILING PROCEDURES Activities of Insured State Banks § 303.121 Filing procedures. (a) Where to file. A notice...

  17. 12 CFR 303.201 - Filing procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Filing procedures. 303.201 Section 303.201 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES OF PRACTICE FILING PROCEDURES Prompt Corrective Action § 303.201 Filing procedures. Applications shall be filed with...

  18. 78 FR 27372 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-10

    ... 2013 Filing to be effective 8/ 1/2013. Filed Date: 5/1/13. Accession Number: 20130501-5062. Comments Due: 5 p.m. ET 5/13/13. Docket Numbers: RP13-863-000. Applicants: Big Sandy Pipeline, LLC. Description: FOSA Modifications May 2013 Filing to be effective 8/ 1/2013. Filed Date: 5/1/13. Accession...

  19. 76 FR 6611 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    .... Description: Williston Basin Interstate Pipeline Company submits tariff filing per 154.204: Non-Conforming... tariff filing per 154.204: Non-Conforming Agreement--Chevron 1-26-11 to be effective 2/1/2011. Filed Date... tariff filing per 154.204: Incorporation of Previously Accepted Tariff Provisions to be effective...

  20. 76 FR 1422 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ... has received the following Natural Gas Pipeline Rate and Refund Report filings: Docket Numbers: RP11... submits tariff filing per 154.312: General Section 4 Rate Case to be effective 2/1/ 2011. Filed Date: 12...: Iroquois Gas Transmission System, L.P. submits tariff filing per 154.204: 12/30/10 Negotiated...

  1. 30 CFR 40.3 - Filing procedures.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Filing procedures. 40.3 Section 40.3 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR FILING AND OTHER ADMINISTRATIVE REQUIREMENTS REPRESENTATIVE OF MINERS § 40.3 Filing procedures. (a) The following information shall be filed by...

  2. 30 CFR 40.3 - Filing procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Filing procedures. 40.3 Section 40.3 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR FILING AND OTHER ADMINISTRATIVE REQUIREMENTS REPRESENTATIVE OF MINERS § 40.3 Filing procedures. (a) The following information shall be filed by...

  3. 5 CFR 2422.5 - Filing petitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Filing petitions. 2422.5 Section 2422.5... FEDERAL LABOR RELATIONS AUTHORITY REPRESENTATION PROCEEDINGS § 2422.5 Filing petitions. (a) Where to file. Petitions must be filed with the Regional Director for the region in which the unit or employee(s) affected...

  4. 77 FR 27044 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-08

    ... Pipeline Company, LP submits tariff filing per 154.204: HK 37731 to Sequent 39880 Capacity Release... Capacity Release Negotiated Rate Agreement filing to be effective 5/1/2012. Filed Date: 4/30/12. Accession....204: HK 37731 to Texla 39887 Capacity Release Negotiated Rate Agreement filing to be effective...

  5. 78 FR 8119 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-05

    ... Revenue Report. Filed Date: 1/22/13. Accession Number: 20130122-5241. Comments Due: 5 p.m. ET 2/4/13... Scheduling Priorities to be effective 4/1/ 2013. Filed Date: 1/24/13. Accession Number: 20130124-5072...: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676...

  6. 77 FR 63809 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ....ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free... Total Penalty Revenue Credits of CenterPoint Energy Gas Transmission Company, LLC. Filed Date: 10/4/12... Revenue Credits of CenterPoint Energy Gas Transmission Company, LLC. ] Filed Date: 10/4/12. Accession...

  7. 78 FR 36181 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-17

    ...-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free). For TTY, call.... Description: RP13-189 Compliance Filing to be effective 4/28/2013. Filed Date: 6/5/13. Accession Number...

  8. 75 FR 47296 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-05

    ...: Negative Rate 2010-07-28 to be effective 8/1/2010. Filed Date: 07/28/2010. Accession Number: 20100728-5157... has received the following Natural Gas Pipeline Rate and Refund Report filings: Docket Numbers: RP10....203: Baseline Filing and Amendment to Non-Conf Agmt and Negotiated Rate Agmt Filing to be effective...

  9. The canonical ensemble via symplectic integrators using Nosé and Nosé-Poincaré chains.

    PubMed

    Leimkuhler, Benedict J; Sweet, Christopher R

    2004-07-01

    Simulations that sample from the canonical ensemble can be generated by the addition of a single degree of freedom, provided that the system is ergodic, as described by Nosé with subsequent modifications by Hoover to allow sampling in real time. Nosé-Hoover dynamics is not ergodic for small or stiff systems and the addition of auxiliary thermostats is needed to overcome this deficiency. Nosé-Hoover dynamics, like its derivatives, does not have a Hamiltonian structure, precluding the use of symplectic integrators which are noted for their long term stability and structure preservation. As an alternative to Nosé-Hoover, the Hamiltonian Nosé-Poincaré method was proposed by Bond, Laird, and Leimkuhler [J. Comput. Phys. 151, 114 (1999)], but the straightforward addition of thermostatting chains does not sample from the canonical ensemble. In this paper a method is proposed whereby additional thermostats can be applied to a Hamiltonian system while retaining sampling from the canonical ensemble. This technique has been used to construct thermostatting chains for the Nosé and Nosé-Poincaré methods. (c) 2004 American Institute of Physics.

  10. Association of common eNOS/NOS3 polymorphisms with preeclampsia in Tunisian Arabs.

    PubMed

    Ben Ali Gannoun, Marwa; Zitouni, Hedia; Raguema, Nozha; Maleh, Wided; Gris, Jean-Christophe; Almawi, Wassim; Mahjoub, Touhami

    2015-09-15

    We investigated the association of endothelial nitric oxide synthase (NOS3) polymorphisms -786T>C, 27-bp repeat 4b/4a, and Glu298Asp with preeclampsia (PE). This was a case-control study involving 345 unrelated Tunisian women with PE and 289 unrelated age- and ethnically matched control women. The -786C allele was significantly increased in PA patients when compared to healthy controls (P=0.015). In contrast, MAF of Glu298Asp (P=0.103) and 4b/4a (P=0.168) were not significantly different between the study groups. Higher frequencies of heterozygous Glu298/298Asp and homozygous -786T/-786T genotypes were seen in PE cases compared to healthy subjects. The combination of genotypes 221 (-786T>C, Glu298Asp, 4a/4a) was more in PE cases compared with control women (17.68% vs. 8.36%; P=0.029). Multivariate regression analysis confirmed this association. Genetic variation at the NOS3 locus represents a genetic risk factor for increased susceptibility to PE.

  11. Virtual file system for PSDS

    NASA Technical Reports Server (NTRS)

    Runnels, Tyson D.

    1993-01-01

    This is a case study. It deals with the use of a 'virtual file system' (VFS) for Boeing's UNIX-based Product Standards Data System (PSDS). One of the objectives of PSDS is to store digital standards documents. The file-storage requirements are that the files must be rapidly accessible, stored for long periods of time - as though they were paper, protected from disaster, and accumulative to about 80 billion characters (80 gigabytes). This volume of data will be approached in the first two years of the project's operation. The approach chosen is to install a hierarchical file migration system using optical disk cartridges. Files are migrated from high-performance media to lower performance optical media based on a least-frequency-used algorithm. The optical media are less expensive per character stored and are removable. Vital statistics about the removable optical disk cartridges are maintained in a database. The assembly of hardware and software acts as a single virtual file system transparent to the PSDS user. The files are copied to 'backup-and-recover' media whose vital statistics are also stored in the database. Seventeen months into operation, PSDS is storing 49 gigabytes. A number of operational and performance problems were overcome. Costs are under control. New and/or alternative uses for the VFS are being considered.

  12. The effect of high protein diet and exercise on irisin, eNOS, and iNOS expressions in kidney.

    PubMed

    Tastekin, Ebru; Palabiyik, Orkide; Ulucam, Enis; Uzgur, Selda; Karaca, Aziz; Vardar, Selma Arzu; Yilmaz, Ali; Aydogdu, Nurettin

    2016-08-01

    Long-term effects of high protein diets (HPDs) on kidneys are still not sufficiently studied. Irisin which increases oxygen consumption and thermogenesis in white fat cells was shown in skeletal muscles and many tissues. Nitric oxide synthases (NOS) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. We aimed to investigate the effects of HPD, irisin and NO expression in kidney and relation of them with exercise and among themselves. Animals were grouped as control, exercise, HPD and exercise combined with HPD (exercise-HPD). Rats were kept on a HPD for 5 weeks and an exercise program was given them as 5 exercise and 2 rest days per week exercising on a treadmill with increasing speed and angle. In our study, while HPD group had similar total antioxidant capacity (TAC) levels with control group, exercise and exercise-HPD groups had lower levels (p < 0.05). Kidneys of exercising rats had no change in irisin or eNOS expression but their iNOS expression had increased (p < 0.001). HPD-E group has not been observed to cause kidney damage and not have a significant effect on rat kidney irisin, eNOS, or iNOS expression. Localization of irisin, eNOS, and iNOS staining in kidney is highly selective and quite clear in this study. Effects of exercise and HPD on kidney should be evaluated with different exercise protocols and contents of the diet. İrisin, eNOS, and iNOS staining localizations should be supported with various research studies.

  13. Differential roles of iNOS and nNOS at rostral ventrolateral medulla during experimental endotoxemia in the rat.

    PubMed

    Chan, J Y; Wang, S H; Chan, S H

    2001-01-01

    We investigated the differential contribution of inducible and neuronal nitric oxide synthase (iNOS and nNOS) at the rostral ventrolateral medulla (RVLM) to endotoxemia induced by E. coli lipopolysaccharide (LPS). In Sprague-Dawley rats maintained under propofol anesthesia, i.v. administration of LPS (15, 30, or 45 mg/kg) induced a reduction (phase I), followed by an augmentation (phase II) and a secondary decrease (phase III) in the power density of the vasomotor components (0-0.8 Hz) in systemic arterial pressure (SAP) signals. LPS also induced an immediate hypotension, followed by a rebound increase and a secondary decrease in SAP. In addition, the level of iNOS mRNA exhibited a significant surge that began with phase I endotoxemia, reaching progressively its peak at phase III. Discernible down-regulation of nNOS mRNA was not detected until the last phase of endotoxemia. Pretreatment with microinjection of the selective iNOS inhibitor, aminoguanidine (250 pmol), into the bilateral RVLM significantly prolonged phases II and III endotoxemia, blunted the initial and secondary hypotension, and antagonized the upregulation of iNOS mRNA. Similar pretreatment with the selective nNOS inhibitor, 7-nitroindazole (1 pmol), on the other hand, discernibly shortened phase II and prolonged phase III endotoxemia, and induced progressive hypotension by antagonizing the rebound increase in SAP. We conclude that the relative prevalence of functional expression and molecular synthesis of iNOS over nNOS in the RVLM may be a crucial determinant for the reduction or loss in power density of the vasomotor components of SAP signals during experimental endotoxemia.

  14. eNOS/iNOS and endoplasmic reticulum stress-induced apoptosis in the placentas of patients with preeclampsia

    PubMed Central

    Du, L; He, F; Kuang, L; Tang, W; Li, Y; Chen, D

    2017-01-01

    Disruption of nitric oxide pathway and endoplasmic reticulum (ER) stress had been observed in preeclampsia (PE). However, the correlation and overall detailed expression profiles of ER stress-related markers and endothelial nitric oxide synthase/inducible nitric oxide synthase (eNOS/iNOS) in patients with PE were poorly understood. In this study, placental protein expression of ER stress-related markers as well as eNOS/iNOS in normotensive control (n=32) and PE pregnancies (n=32) was examined by western blot. In addition, apoptosis was detected by terminal deoxynucleotidyl transferase-mediated nick-end labelling (TUNEL) staining in placentas. Compared with control, we found elevated ER stress response was agreeable with iNOS upregulation in placenta tissue of PE patients. Placental protein expression of ER stress-related markers, including GRP78, GRP94, p-PERK, eIF2a, p-eIF2a, XBP1, CHOP, Ire1, p-Ire1 and iNOS, was higher, and eNOS expression was lower in PE (P<0.05 for all); however, the expression of ATF6 and PERK was similar in the PE and control groups. Upregulation of CHOP and iNOS was consistent of apoptosis increasing indicated by TUNEL staining and caspase 4 expression upregulation in PE placenta. Our datas suggest that the exaggerated ER stress response and upregulated iNOS are probably associated with increased apoptosis in placenta of PE patients and may contribute to the pathophysiology of PE. PMID:27030287

  15. Regulation of iNOS-Derived ROS Generation by HSP90 and Cav-1 in Porcine Reproductive and Respiratory Syndrome Virus-Infected Swine Lung Injury.

    PubMed

    Yan, Meiping; Hou, Make; Liu, Jie; Zhang, Songlin; Liu, Bang; Wu, Xiaoxiong; Liu, Guoquan

    2017-08-01

    In the lungs, endothelial nitric oxide synthase (eNOS) is usually expressed in endothelial cells and inducible nitric oxide synthase (iNOS) is mainly expressed in alveolar macrophages and epithelial cells. Both eNOS and iNOS are involved in lung inflammation. While they play several roles in lung inflammation formation and resolution, their expression and activity are also regulated by inflammatory factors. Their expression relationship in virus infection-induced lung injury is not well addressed. In this report, we analyzed expression of both eNOS and iNOS, the production of nitric oxide (NO) and reactive oxygen species (ROS), and expression of their associated regulatory proteins, heat shock protein 90 (HSP90) and caveolin-1 (Cav-1), in a swine lung injury model induced by porcine reproductive and respiratory syndrome virus (PRRSV) infection. The combination of upregulation of iNOS and downregulation of eNOS was observed in both natural and experimental PRRSV-infected lungs, while the combination is much enhanced in natural infected lungs. While NO production is much reduced in both infections, ROS was enhanced only in natural infected lungs. Moreover, HSP90 is increased in both natural and experimental infection and less Cav-1 expressed was observed only in the natural PRRSV-infected lungs. Therefore, the increased ROS generation is likely due to the increased iNOS and its unbalanced regulation by HSP90 and Cav-1, and it also likely causes higher endothelial dysfunction in clinical PRRSV-infected lungs.

  16. The Brain NO Levels and NOS Activities Ascended in the Early and Middle Stages and Descended in the Terminal Stage in Scrapie-Infected Animal Models.

    PubMed

    Chen, Li-Na; Sun, Jing; Yang, Xiao-Dong; Xiao, Kang; Lv, Yan; Zhang, Bao-Yun; Zhou, Wei; Chen, Cao; Gao, Chen; Shi, Qi; Dong, Xiao-Ping

    2017-04-01

    The infections of prion agents may cause progressive and fatal neurodegenerative diseases in humans and a serial of animal species. Previous studies have proposed that the levels of nitric oxide (NO) and nitric oxide synthase (NOS) in the brains of some neurodegeneration diseases changed, while S-nitrosylation (SNO) of many brain proteins altered in prion diseases. To elucidate the potential changes of brain NO levels during prion infection, the NO levels and NOS activities in the brain tissues of three scrapie experimental rodents were measured, including scrapie agent 263 K-infected hamsters and 139A- and ME7-infected mice. Both NO levels and NOS activities, including total NOS (TNOS) and inducible NOS (iNOS), were increased at the terminal stages of scrapie-infected animals. Assays of the brain samples collected at different time points during scrapie infection showed that the NO levels and NOS activities started to increase at early stage, reached to the peak in the middle stage, and dropped down at late stage. Western blots for brain iNOS revealed increased firstly and decreased late, especially in the brains of 139A- and ME7-infected mice. In line with those alterations, the levels of the SNO forms of several selected brain proteins such as aquaporin-1 (AQP1), calcium/calmodulin-dependent protein kinase II (CaMKII), neurogranin, and opalin, underwent similar changing trends, while their total protein levels did not change obviously during scrapie infection. Our data here for the first time illustrate the changing profile of brain NO and NOS during prion infection. Time-dependent alterations of brain NO level and the associated protein S-nitrosylation process may contribute greatly to the neuropathological damage in prion diseases.

  17. 21 CFR 516.163 - Change in ownership of an index file.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS NEW ANIMAL DRUGS FOR MINOR USE AND MINOR SPECIES Index of Legally Marketed Unapproved New Animal Drugs for Minor Species § 516.163 Change in ownership of an... owner shall submit in writing to FDA a statement that all rights in the index file have been transferred...

  18. 29 CFR 4007.3 - Filing requirement; method of filing.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... site (www.pbgc.gov). Subject to the provisions of § 4007.13, the plan administrator of each covered... does not apply to information filed to comply with a PBGC request under (4007.10(c) (dealing with...

  19. Hazardous Materiel Data File (HMDF), quarterly update. Data file

    SciTech Connect

    Not Available

    1989-05-01

    The Hazardous Materiel Data File's purpose is to provide storage and transportation information on chemical, radioactive, and ammunition items in the Army inventory. Data are taken from the Army Central Logistics Data Bank.

  20. Hazardous Materiel Data File (HMDF), quarterly update. Data file

    SciTech Connect

    Not Available

    1990-05-01

    The Hazardous Materiel Data File (HMDF) purpose is to provide storage and transportation information on chemical, radioactive and ammunition items in the Army inventory. Data are taken from the Army Central Logistics Data Bank (ACLDB).

  1. Species concepts and species delimitation.

    PubMed

    De Queiroz, Kevin

    2007-12-01

    The issue of species delimitation has long been confused with that of species conceptualization, leading to a half century of controversy concerning both the definition of the species category and methods for inferring the boundaries and numbers of species. Alternative species concepts agree in treating existence as a separately evolving metapopulation lineage as the primary defining property of the species category, but they disagree in adopting different properties acquired by lineages during the course of divergence (e.g., intrinsic reproductive isolation, diagnosability, monophyly) as secondary defining properties (secondary species criteria). A unified species concept can be achieved by treating existence as a separately evolving metapopulation lineage as the only necessary property of species and the former secondary species criteria as different lines of evidence (operational criteria) relevant to assessing lineage separation. This unified concept of species has several consequences for species delimitation, including the following: First, the issues of species conceptualization and species delimitation are clearly separated; the former secondary species criteria are no longer considered relevant to species conceptualization but only to species delimitation. Second, all of the properties formerly treated as secondary species criteria are relevant to species delimitation to the extent that they provide evidence of lineage separation. Third, the presence of any one of the properties (if appropriately interpreted) is evidence for the existence of a species, though more properties and thus more lines of evidence are associated with a higher degree of corroboration. Fourth, and perhaps most significantly, a unified species concept shifts emphasis away from the traditional species criteria, encouraging biologists to develop new methods of species delimitation that are not tied to those properties.

  2. 78 FR 75554 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-12

    ...-Conforming to be effective 1/1/2014. Filed Date: 11/27/13. Accession Number: 20131127-5063. Comments Due: 5 p.... Description: Clean-up Filing to be effective 1/1/2014. Filed Date: 11/27/13. Accession Number: 20131127-5076... Company, Ltd. Description: Young Fuel Reimbursement Filing to be effective 1/1/ 2014. Filed Date: 11/27/13...

  3. 77 FR 25713 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-01

    ... Interstate Company, L.L.C. Description: Permanent Release Filing to be effective 5/24/2012. Filed Date: 4/23... be effective 5/21/2012. Filed Date: 4/20/12. Accession Number: 20120420-5235. Comments Due: 5 p.m. ET... Negotiated Rate Agreement 31033 filing to be effective 4/1/2012. Filed Date: 4/23/12. Accession Number...

  4. 77 FR 34376 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    .../docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free). For TTY... Sequent 39962 Cap Rel Neg Rate Agmt filing to be effective 6/2/2012. Filed Date: 6/4/12. Accession Number.../1/2010. Filed Date: 6/4/12. Accession Number: 20120604-5095. Comments Due: 5 p.m. ET 6/18/12. Any...

  5. Interior view of connector between Building Nos. 608 & 609 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of connector between Building Nos. 608 & 609 (HABS CO-172-DS) facing north - Fitzsimons General Hospital, Neuropsychiatric Ward, Southeast Corner of East Nineteenth Avenue & Wheeling Street (formerly South Van Valzuh Street), Aurora, Adams County, CO

  6. 36. ISLAND PLANT: Nos. 1 AND 2 TWENTYSIX INCH HORIZONTAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. ISLAND PLANT: Nos. 1 AND 2 TWENTY-SIX INCH HORIZONTAL SAMSON TURBINES - American Falls Water, Power & Light Company, Island Power Plant, Snake River, below American Falls Dam, American Falls, Power County, ID

  7. 37. ISLAND PLANT: Nos. 1 AND 2 TWENTYSIX INCH SPECIAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    37. ISLAND PLANT: Nos. 1 AND 2 TWENTY-SIX INCH SPECIAL HORIZONTAL SAMSON TURBINE (RIVITED CASE) - American Falls Water, Power & Light Company, Island Power Plant, Snake River, below American Falls Dam, American Falls, Power County, ID

  8. Interior of Left Powerhouse showing generator Nos. 14. This view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior of Left Powerhouse showing generator Nos. 1-4. This view is from the catwalk at the level of the overhead crane, looking west. - Columbia Basin Project, Grand Coulee Dam Powerplant Complex, Grand Coulee, Grant County, WA

  9. 9. DETAIL OF BENTS NOS 3 AND 4. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. DETAIL OF BENTS NOS 3 AND 4. VIEW TO NORTH-NORTHEAST - Milwaukee Road Railroad Overpass, Spanning Chicago, Milwaukee, St. Paul, & Pacific Railroad Grade (Milwaukee Road) at Orange Street, Missoula, Missoula County, MT

  10. 11. BENTS NOS. 3 AND 2. VIEW TO SOUTHSOUTHWEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. BENTS NOS. 3 AND 2. VIEW TO SOUTH-SOUTHWEST - Milwaukee Road Railroad Overpass, Spanning Chicago, Milwaukee, St. Paul, & Pacific Railroad Grade (Milwaukee Road) at Orange Street, Missoula, Missoula County, MT

  11. 5. VIEW OF NORTH SECTION OF STRUCTURE SHOWING BRIDGE NOS. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. VIEW OF NORTH SECTION OF STRUCTURE SHOWING BRIDGE NOS. 10 AND 9 FROM CENTRAL STAIRWAY. LOOKING NORTH. - Greenville Yard, Transfer Bridge System, Port of New York/New Jersey, Upper New York Bay, Jersey City, Hudson County, NJ

  12. 6. VIEW OF SOUTH SECTION OF STRUCTURE SHOWING BRIDGE NOS. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW OF SOUTH SECTION OF STRUCTURE SHOWING BRIDGE NOS. 12, 13, AND 14 FROM CENTRAL STAIRWAY. LOOKING SOUTH. - Greenville Yard, Transfer Bridge System, Port of New York/New Jersey, Upper New York Bay, Jersey City, Hudson County, NJ

  13. Vault Area (original section), east corridor, looking north (Vault Nos. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Vault Area (original section), east corridor, looking north (Vault Nos. 1-9 - Fort McNair, Film Store House, Fort Lesley J. McNair, P Street between Third & Fourth Streets, Southwest, Washington, District of Columbia, DC

  14. Los Angeles County Poor Farm, Patient Ward Nos. 210 & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Los Angeles County Poor Farm, Patient Ward Nos. 210 & 211 - Type B Plan, 7601 Imperial Highway; bounded by Esperanza Street, Laurel Street, Flores Street, and Descanso Street, Downey, Los Angeles County, CA

  15. Investigating the Role of TNF-α and IFN-γ Activation on the Dynamics of iNOS Gene Expression in LPS Stimulated Macrophages

    PubMed Central

    Salim, Taha; Sershen, Cheryl L.; May, Elebeoba E.

    2016-01-01

    Macrophage produced inducible nitric oxide synthase (iNOS) is known to play a critical role in the proinflammatory response against intracellular pathogens by promoting the generation of bactericidal reactive nitrogen species. Robust and timely production of nitric oxide (NO) by iNOS and analogous production of reactive oxygen species are critical components of an effective immune response. In addition to pathogen associated lipopolysaccharides (LPS), iNOS gene expression is dependent on numerous proinflammatory cytokines in the cellular microenvironment of the macrophage, two of which include interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α). To understand the synergistic effect of IFN-γ and TNF-α activation, and LPS stimulation on iNOS expression dynamics and NO production, we developed a systems biology based mathematical model. Using our model, we investigated the impact of pre-infection cytokine exposure, or priming, on the system. We explored the essentiality of IFN-γ priming to the robustness of initial proinflammatory response with respect to the ability of macrophages to produce reactive species needed for pathogen clearance. Results from our theoretical studies indicated that IFN-γ and subsequent activation of IRF1 are essential in consequential production of iNOS upon LPS stimulation. We showed that IFN-γ priming at low concentrations greatly increases the effector response of macrophages against intracellular pathogens. Ultimately the model demonstrated that although TNF-α contributed towards a more rapid response time, measured as time to reach maximum iNOS production, IFN-γ stimulation was significantly more significant in terms of the maximum expression of iNOS and the concentration of NO produced. PMID:27276061

  16. eNOS-uncoupling in age-related erectile dysfunction

    PubMed Central

    Johnson, JM; Bivalacqua, TJ; Lagoda, GA; Burnett, AL; Musicki, B

    2011-01-01

    Aging is associated with ED. Although age-related ED is attributed largely to increased oxidative stress and endothelial dysfunction in the penis, the molecular mechanisms underlying this effect are not fully defined. We evaluated whether endothelial nitric oxide synthase (eNOS) uncoupling in the aged rat penis is a contributing mechanism. Correlatively, we evaluated the effect of replacement with eNOS cofactor tetrahydrobiopterin (BH4) on erectile function in the aged rats. Male Fischer 344 ‘young’ (4-month-old) and ‘aged’ (19-month-old) rats were treated with a BH4 precursor sepiapterin (10 mg/kg intraperitoneally) or vehicle for 4 days. After 1-day washout, erectile function was assessed in response to electrical stimulation of the cavernous nerve. Endothelial dysfunction (eNOS uncoupling) and oxidative stress (thiobarbituric acid reactive substances, TBARS) were measured by conducting western blot in penes samples. Erectile response was significantly reduced in aged rats, whereas eNOS uncoupling and TBARS production were significantly increased in the aged rat penis compared with young rats. Sepiapterin significantly improved erectile response in aged rats and prevented increase in TBARS production, but did not affect eNOS uncoupling in the penis of aged rats. These findings suggest that aging induces eNOS uncoupling in the penis, resulting in increased oxidative stress and ED. PMID:21289638

  17. Defining viral species: making taxonomy useful.

    PubMed

    Peterson, A Townsend

    2014-07-23

    Virus taxonomy at present is best characterized as a categorization of convenience, without a firm basis in the principles of evolutionary biology. Specifically, virus species definitions appear to depend more on tradition and popular opinion among virologists than on firm, quantitative biological evidence. I suggest a series of changes to underlying species concepts that would shift the field from one that simply files viruses away in taxonomic boxes to one that can learn important biological lessons from its taxonomy.

  18. Direct evidence of iNOS-mediated in vivo free radical production and protein oxidation in acetone-induced ketosis.

    PubMed

    Stadler, Krisztian; Bonini, Marcelo G; Dallas, Shannon; Duma, Danielle; Mason, Ronald P; Kadiiska, Maria B

    2008-08-01

    Diabetic patients frequently encounter ketosis that is characterized by the breakdown of lipids with the consequent accumulation of ketone bodies. Several studies have demonstrated that reactive species are likely to induce tissue damage in diabetes, but the role of the ketone bodies in the process has not been fully investigated. In this study, electron paramagnetic resonance (EPR) spectroscopy combined with novel spin-trapping and immunological techniques has been used to investigate in vivo free radical formation in a murine model of acetone-induced ketosis. A six-line EPR spectrum consistent with the alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone radical adduct of a carbon-centered lipid-derived radical was detected in the liver extracts. To investigate the possible enzymatic source of these radicals, inducible nitric oxide synthase (iNOS) and NADPH oxidase knockout mice were used. Free radical production was unchanged in the NADPH oxidase knockout but much decreased in the iNOS knockout mice, suggesting a role for iNOS in free radical production. Longer-term exposure to acetone revealed iNOS overexpression in the liver together with protein radical formation, which was detected by confocal microscopy and a novel immunospin-trapping method. Immunohistochemical analysis revealed enhanced lipid peroxidation and protein oxidation as a consequence of persistent free radical generation after 21 days of acetone treatment in control and NADPH oxidase knockout but not in iNOS knockout mice. Taken together, our data demonstrate that acetone administration, a model of ketosis, can lead to protein oxidation and lipid peroxidation through a free radical-dependent mechanism driven mainly by iNOS overexpression.

  19. Direct evidence of iNOS-mediated in vivo free radical production and protein oxidation in acetone-induced ketosis

    PubMed Central

    Stadler, Krisztian; Bonini, Marcelo G.; Dallas, Shannon; Duma, Danielle; Mason, Ronald P.; Kadiiska, Maria B.

    2008-01-01

    Diabetic patients frequently encounter ketosis that is characterized by the breakdown of lipids with the consequent accumulation of ketone bodies. Several studies have demonstrated that reactive species are likely to induce tissue damage in diabetes, but the role of the ketone bodies in the process has not been fully investigated. In this study, electron paramagnetic resonance (EPR) spectroscopy combined with novel spin-trapping and immunological techniques has been used to investigate in vivo free radical formation in a murine model of acetone-induced ketosis. A six-line EPR spectrum consistent with the α-(4-pyridyl-1-oxide)-N-t-butylnitrone radical adduct of a carbon-centered lipid-derived radical was detected in the liver extracts. To investigate the possible enzymatic source of these radicals, inducible nitric oxide synthase (iNOS) and NADPH oxidase knockout mice were used. Free radical production was unchanged in the NADPH oxidase knockout but much decreased in the iNOS knockout mice, suggesting a role for iNOS in free radical production. Longer-term exposure to acetone revealed iNOS overexpression in the liver together with protein radical formation, which was detected by confocal microscopy and a novel immunospin-trapping method. Immunohistochemical analysis revealed enhanced lipid peroxidation and protein oxidation as a consequence of persistent free radical generation after 21 days of acetone treatment in control and NADPH oxidase knockout but not in iNOS knockout mice. Taken together, our data demonstrate that acetone administration, a model of ketosis, can lead to protein oxidation and lipid peroxidation through a free radical-dependent mechanism driven mainly by iNOS overexpression. PMID:18559982

  20. Effect of exercise training on eNOS expression, NO production and oxygen metabolism in human placenta.

    PubMed

    Ramírez-Vélez, Robinson; Bustamante, Juanita; Czerniczyniec, Analia; Aguilar de Plata, Ana C; Lores-Arnaiz, Silvia

    2013-01-01

    To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. The study included 20 nulliparous in gestational week 16-20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55-75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1-3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn

  1. Redox signal regulation via nNOS phosphorylation at Ser847 in PC12 cells and rat cerebellar granule neurons.

    PubMed

    Kasamatsu, Shingo; Watanabe, Yasuo; Sawa, Tomohiro; Akaike, Takaaki; Ihara, Hideshi

    2014-04-15

    Phosphorylation is considered a main mechanism modulating nNOS (neuronal nitric oxide synthase) function to reduce NO production. In the present study, the effects of nNOS phosphorylation on redox signalling, including that of NO, ROS (reactive oxygen species), and 8-nitro-cGMP (8-nitroguanosine 3',5'-cyclic monophosphate), a downstream messenger of redox signalling, were investigated. In vitro experiments revealed that a phosphorylation-mimic mutant of nNOS (Ser847 replaced with aspartic acid, 847D) increased uncoupling to produce a superoxide. In addition, nicotine, which triggers an influx of Ca2+, induced more ROS and 8-nitro-cGMP production in 847D-expressing PC12 cells than WT (wild-type)-expressing cells. Additionally, nicotine-induced phosphorylation of nNOS at Ser847 and increased ROS and 8-nitro-cGMP production in rat CGNs (cerebellar granule neurons). In CGNs, the NOS (nitric oxide synthase) inhibitor L-NAME (NG-nitro-L-arginine methyl ester) and superoxide dismutase completely inhibited ROS and 8-nitro-cGMP production, whereas the CaMK (Ca2+/calmodulin-dependent protein kinase) inhibitor KN93 mildly reduced this effect. Nicotine induced HO-1 (haem oxygenase 1) expression in CGNs and showed cytoprotective effects against apoptosis. Moreover, 8-nitro-cGMP treatment showed identical effects that were attenuated by KN93 pre-treatment. The present paper provides the first substantial corroboration for the biological effects of nNOS phosphorylation at Ser847 on redox signalling, including ROS and intracellular 8-nitro-cGMP generation in neurons, which possibly play roles in neuroprotection.

  2. FRS Geospatial Return File Format

    EPA Pesticide Factsheets

    The Geospatial Return File Format describes format that needs to be used to submit latitude and longitude coordinates for use in Envirofacts mapping applications. These coordinates are stored in the Geospatail Reference Tables.

  3. 76 FR 39757 - Filing Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... deemed signed by that registered user. (4) Upon learning of the potential compromise of the... tool, embedded files, embedded sounds or other multimedia); forms actions; JavaScript actions; external...

  4. 76 FR 62092 - Filing Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ... From the Federal Register Online via the Government Publishing Office INTERNATIONAL TRADE... filed with the Commission, reduce Commission expenditures, and conform agency processes to federal government initiatives. DATES: Effective Date: November 7, 2011. FOR FURTHER INFORMATION CONTACT: James...

  5. 43 CFR 4.1391 - Who may file; where to file; when to file; filing of administrative record.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., 801 N. Quincy Street, Suite 300, Arlington, VA 22203 (telephone 703-235-3750). OSM shall file the complete administrative record of the determination under review with the Board as soon as practicable....

  6. The beneficial effects of melatonin against heart mitochondrial impairment during sepsis: inhibition of iNOS and preservation of nNOS.

    PubMed

    Ortiz, Francisco; García, José A; Acuña-Castroviejo, Darío; Doerrier, Carolina; López, Ana; Venegas, Carmen; Volt, Huayqui; Luna-Sánchez, Marta; López, Luis C; Escames, Germaine

    2014-01-01

    While it is accepted that the high production of nitric oxide (NO˙) by the inducible nitric oxide synthase (iNOS) impairs cardiac mitochondrial function during sepsis, the role of neuronal nitric oxide synthase (nNOS) may be protective. During sepsis, there is a significantly increase in the expression and activity of mitochondrial iNOS (i-mtNOS), which parallels the changes in cytosolic iNOS. The existence of a constitutive NOS form (c-mtNOS) in heart mitochondria has been also described, but its role in the heart failure during sepsis remains unclear. Herein, we analyzed the changes in mitochondrial oxidative stress and bioenergetics in wild-type and nNOS-deficient mice during sepsis, and the role of melatonin, a known antioxidant, in these changes. Sepsis was induced by cecal ligation and puncture, and heart mitochondria were analyzed for NOS expression and activity, nitrites, lipid peroxidation, glutathione and glutathione redox enzymes, oxidized proteins, and respiratory chain activity in vehicle- and melatonin-treated mice. Our data show that sepsis produced a similar induction of iNOS/i-mtNOS and comparable inhibition of the respiratory chain activity in wild-type and in nNOS-deficient mice. Sepsis also increased mitochondrial oxidative/nitrosative stress to a similar extent in both mice strains. Melatonin administration inhibited iNOS/i-mtNOS induction, restored mitochondrial homeostasis in septic mice, and preserved the activity of nNOS/c-mtNOS. The effects of melatonin were unrelated to the presence or the absence of nNOS. Our observations show a lack of effect of nNOS on heart bioenergetic impairment during sepsis and further support the beneficial actions of melatonin in sepsis.

  7. File servers, networking, and supercomputers

    NASA Technical Reports Server (NTRS)

    Moore, Reagan W.

    1992-01-01

    One of the major tasks of a supercomputer center is managing the massive amount of data generated by application codes. A data flow analysis of the San Diego Supercomputer Center is presented that illustrates the hierarchical data buffering/caching capacity requirements and the associated I/O throughput requirements needed to sustain file service and archival storage. Usage paradigms are examined for both tightly-coupled and loosely-coupled file servers linked to the supercomputer by high-speed networks.

  8. File servers, networking, and supercomputers

    NASA Technical Reports Server (NTRS)

    Moore, Reagan W.

    1991-01-01

    One of the major tasks of a supercomputer center is managing the massive amount of data generated by application codes. A data flow analysis of the San Diego Supercomputer Center is presented that illustrates the hierarchical data buffering/caching capacity requirements and the associated I/O throughput requirements needed to sustain file service and archival storage. Usage paradigms are examined for both tightly-coupled and loosely-coupled file servers linked to the supercomputer by high-speed networks.

  9. Files in an Interactive Environment.

    DTIC Science & Technology

    1980-04-01

    programs that exploit the existing file system and leaves the limitations to be dealt with by the user. In contrast, in the VM/ CMS environment, almost all...described here have been implemented using SIMULA-67 in the VM/ CMS operating system . The code con- sists of approximately 1500 lines of SIMULA of which...the SIMULA run time system code which is inadequate in this environment and the SIMULA code makes it possible to associate CMS DD names with CMS files

  10. Directory of astronomical data files

    NASA Technical Reports Server (NTRS)

    1978-01-01

    This Directory of Astronomical Data Files was prepared by the Data Task Force of the Interagency Coordination Committee for Astronomy (ICCA) in cooperation with the National Space Science Data Center (NSSDC). The purpose of the Directory is to provide a listing which will enable a user to locate stellar and extragalactic data sources keyed along with sufficient descriptive information to permit him to assess the value of the files for his use as well as the status and availability of the compilations.

  11. In vitro and in vivo induction and activation of nNOS by LPS in oligodendrocytes

    PubMed Central

    Yao, SY.; Ljunggren-Rose, A.; Chandramohan, N.; Whetsell, W.O.; Sriram, S.

    2014-01-01

    There are currently four known isoforms of nitric oxide synthase (NOS). Of these, neuronal NOS (nNOS) is known to be present exclusively in neurons, endothelial NOS (eNOS) in vascular endothelium, while the inducible form of NOS (iNOS) is known to be activated in oligodendrocytes, astrocytes and microglia. The fourth isoform, mitochondrial NOS (mtNOS), represents a post translational modification of nNOS. Using western blotting and real time-PCR, we show induction and activation of nNOS following culture of oligodendrocyte progenitor cells (OPC) with lipopolysaccharide (LPS). Activation of nNOS results in accumulation of peroxynitrite and tyrosine nitration of proteins in oligodendrocytes resulting in reduced cell viability. Injection of LPS in vivo into the corpus callosum of rats leads to the development of extensive demyelination of the white matter tracts. Immunostaining of regions close to the injection site shows the presence of nNOS, but not iNOS, in oligodendrocytes. Neither iNOS nor nNOS was seen in astrocytes in areas of demyelination. These studies suggest that activation of nNOS in oligodendrocytes leads to oligodendrocyte injury resulting in demyelination. PMID:20724006

  12. Safety Evaluation Report related to the operation of Watts Bar Nuclear Plant, Units 1 and 2 (Docket Nos. 50-390 and 50-391). Supplement No. 12

    SciTech Connect

    Tam, P.S.

    1993-10-01

    Supplement No. 12 to the Safety Evaluation Report for the application filed by the Tennessee Valley Authority for license to operate Watts Bar Nuclear Plant, Units 1 and 2, Docket Nos. 50-390 and 50-391, located in Rhea County, Tennessee, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation of (1) additional information submitted by the applicant since Supplement No. 11 was issued, and (2) matters that the staff had under review when Supplement No. 11 was issued.

  13. Safety evaluation report related to the operation of Watts Bar Nuclear Plant, Units 1 and 2 (Docket Nos. 50-390 and 50-391): Supplement No. 19

    SciTech Connect

    1995-11-01

    Supplement No. 19 to the Safety Evaluation Report for the application filed by the Tennessee Valley Authority for license to operate Watts Bar Nuclear Plant, Units 1 and 2, Docket Nos. 50-390 and 50-391, located in Rhea County Tennessee, has been prepared by the Office of Nuclear Reactor Regulation of the Nuclear Regulatory Commission. The purpose of this supplement is to update the Safety Evaluation with (1) additional information submitted by the applicant since Supplement No. 18 was issued, and (2) matters that the staff had under review when Supplement No. 18 was issued.

  14. Zebra: A striped network file system

    NASA Technical Reports Server (NTRS)

    Hartman, John H.; Ousterhout, John K.

    1992-01-01

    The design of Zebra, a striped network file system, is presented. Zebra applies ideas from log-structured file system (LFS) and RAID research to network file systems, resulting in a network file system that has scalable performance, uses its servers efficiently even when its applications are using small files, and provides high availability. Zebra stripes file data across multiple servers, so that the file transfer rate is not limited by the performance of a single server. High availability is achieved by maintaining parity information for the file system. If a server fails its contents can be reconstructed using the contents of the remaining servers and the parity information. Zebra differs from existing striped file systems in the way it stripes file data: Zebra does not stripe on a per-file basis; instead it stripes the stream of bytes written by each client. Clients write to the servers in units called stripe fragments, which are analogous to segments in an LFS. Stripe fragments contain file blocks that were written recently, without regard to which file they belong. This method of striping has numerous advantages over per-file striping, including increased server efficiency, efficient parity computation, and elimination of parity update.

  15. Invasive Species

    EPA Pesticide Factsheets

    Invasive species have significantly changed the Great Lakes ecosystem. An invasive species is a plant or animal that is not native to an ecosystem, and whose introduction is likely to cause economic, human health, or environmental damage.

  16. 78 FR 19217 - Endangered Species; File No. 16547-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    ... above and below 22 parts per thousand salinity. Researchers are currently authorized to capture adult... telemetry tags dependent on the life stage (adult, sub-adult and juvenile) and the salinity level where... salinity level in the waters of Virginia and Maryland. All previous activities are authorized; however, the...

  17. 77 FR 73024 - Endangered Species; File No. 16547-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-07

    ... parts per thousand salinity. Researchers are currently authorized to capture adult, juvenile and early..., sub-adult and juvenile) and the salinity level where captured; or ] also externally satellite tagged... with either internal or external telemetry tags without respect to salinity level of the Chesapeake Bay...

  18. 77 FR 61745 - Endangered Species; File No. 16803

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-11

    ... ratio, health status, diving behavior, local movements, habitat use, and migration routes. Up to 50... attachment of up to two transmitters. Animals with transmitters may be tracked by vessel after release...

  19. 76 FR 77781 - Endangered Species; File No. 15802

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-14

    ... authorization to capture green (Chelonia mydas), hawksbill (Eretmochelys imbricata), Kemp's ridley (Lepidochelys kempii), leatherback (Dermochelys coriacea), and loggerhead (Caretta caretta) sea turtles. Sea...

  20. 76 FR 33245 - Endangered Species; File No. 15135

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... turtles, 5 Kemp's ridleys, 5 loggerheads, 15 greens, 2 hawksbills, and 2 leatherbacks may be mortalities... Isle, NC 28594, has been issued a permit to take threatened and endangered sea turtles for purposes... Register (75 FR 11863) that a request for a scientific research permit to take sea turtles had...

  1. 78 FR 13642 - Endangered Species; File No. 17506

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... captures, green, loggerhead, hawksbill, Kemp's ridley, and leatherback sea turtles would be counted during... to take green (Chelonia mydas), loggerhead (Caretta caretta), hawksbill (Eretmochelys imbricata), Kemp's ridley (Lepidochelys kempii), and leatherback (Dermochelys coriaceae) sea turtles for...

  2. 76 FR 4635 - Endangered Species; File No. 15552

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ..., loggerhead, hawksbill, leatherback, Kemp's ridley, olive ridley, and unidentified hardshell sea turtles by..., has applied in due form for a permit to take green (Chelonia mydas), hawksbill (Eretmochelys imbricata...), leatherback (Dermochelys coriacea), and unidentified hardshell sea turtles for purposes of scientific...

  3. 76 FR 45781 - Endangered Species; File No. 15552

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... to take green (Chelonia mydas), hawksbill (Eretmochelys imbricata), loggerhead (Caretta caretta... coriacea), and ] unidentified hardshell sea turtles for the purposes of scientific research. ADDRESSES: The... a scientific research permit to take green, loggerhead, hawksbill, leatherback, Kemp's ridley,...

  4. 77 FR 27718 - Endangered Species; File No. 15566

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... coriacea), and 1 hawksbill (Eretmochelys imbricata) sea turtle to assess temporal change in catch rates, size distributions, sex and genetic ratios, and health of sea turtles. Captures occur annually in... leatherback, and one hawksbill sea turtle could be accidentally killed over the life of the permit. Permit...

  5. 78 FR 26323 - Endangered Species; File No. 17183

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-06

    ...), hawksbill (Eretmochelys imbricata), and Kemp's ridley (Lepidochelys kempii) sea turtles for purposes of... on the demographics and movements of green, loggerhead, hawksbill, and Kemp's ridley sea turtles off... 39220) that a request for a scientific research permit to take green, loggerhead, hawksbill, and...

  6. 75 FR 9868 - Endangered Species; File No. 14622

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-04

    ... aggregations of loggerhead, Kemp's ridley, and hawksbill sea turtles; and (3) determine the movements... hawksbill sea turtles annually during capture- mark-recapture studies. Captured turtles would be examined..., Jacksonville, FL 32218, has applied in due form for a permit to take green (Chelonia mydas),...

  7. 76 FR 78890 - Endangered Species; File No. 15566

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    ... (Chelonia mydas), 1 leatherback (Dermochelys coriacea), and 1 hawksbill (Eretmochelys imbricata) sea turtle... loggerhead, one Kemp's ridley, one green, one leatherback, and one hawksbill sea turtle could be accidentally... health of sea turtles. Captures occur annually in coastal waters between Winyah Bay, SC and St....

  8. 77 FR 54566 - Endangered Species; File No. 16134

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ... (Chelonia mydas), Kemp's ridley (Lepidochelys kempii), hawksbill (Eretmochelys imbricata), leatherback (Dermochelys coriacea), and loggerhead (Caretta caretta) sea turtles for purposes of scientific research... 222-226). The five-year permit authorizes research on leatherback, loggerhead, green, hawksbill,...

  9. 78 FR 57353 - Endangered Species; File No. 14726

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-18

    ... (Lepidochelys kempii), hawksbill (Eretmochelys imbricata), and leatherback (Dermochelys coriacea) sea turtles, to quantify threats to pelagic sea turtles, and to gather information on their life-history, genetics... integrated transponder tag, measure, weigh, and oral swab sea turtles. A subset of animals may be...

  10. 76 FR 76950 - Endangered Species; File No. 16134

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-09

    ... permit to conduct research on leatherback, loggerhead, green, hawksbill, and Kemp's ridley sea turtles in... a permit to take green (Chelonia mydas), Kemp's ridley (Lepidochelys kempii), hawksbill (Eretmochelys imbricata), leatherback (Dermochelys coriacea), and loggerhead (Caretta caretta) sea turtles...

  11. 76 FR 18725 - Endangered Species; File No. 16174

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    ... permit to take green sea turtles (Chelonia mydas) for purposes of scientific research. ] DATES: Written.... Annually, up to 30 green sea turtles would be captured by hand, transported to shore, measured, weighed...

  12. 75 FR 19363 - Endangered Species; File No. 14754

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... 25,000 fertilized shortnose sturgeon eggs of Saint John River ancestry from Acadian Sturgeon and Caviar Inc., Saint John, NB, Canada. The initial proposed research will take place during two sampling.... Dated: April 7, 2010. Jolie Harrison, Acting Chief, Permits, Conservation and Education Division,...

  13. 75 FR 61424 - Endangered Species; File No. 15596

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... educational display of five captive-bred, non-releaseable adult shortnose sturgeon. This display would be used... any release of captive sturgeon into the wild. The permit is requested for a duration of 5 years...

  14. 76 FR 27306 - Endangered Species; File No. 15661

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-11

    ...; fax (301) 713-0376; and Pacific Islands Region, NMFS, 1601 Kapiolani Blvd., Rm 1110, Honolulu, HI... patterns for green and hawksbill sea turtles in the Northern Mariana Islands with an eye toward identifying... and 50 hawksbill sea turtles would be captured, measured, weighed, flipper tagged, passive integrated...

  15. 77 FR 55194 - Endangered Species; File No. 17095

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-07

    .... ACTION: Issuance of permit. SUMMARY: Notice is hereby given that Entergy Nuclear Operations Inc., 450... purposes of scientific research. ADDRESSES: The permit and related documents are available for review upon... Federal Register (77 FR 21750) that a request for a scientific research permit to take shortnose sturgeon...

  16. 78 FR 17359 - Endangered Species; File No. 17095-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-21

    ... Operations Inc., 450 Broadway, Suite 3, Buchanan, NY 10511 [Responsible Party: John Ventosa], has been issued... (Acipenser oxyrinchus oxyrinchus) for purposes of scientific research. ADDRESSES: The permit modification and... scientific research permit modification to take shortnose sturgeon and Atlantic sturgeon had been submitted...

  17. 75 FR 22106 - Endangered Species; File No. 14510

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-27

    ... (Chelonia mydas), loggerhead (Caretta caretta), olive ridley (Lepidochelys olivacea), and leatherback..., inject tetracycline, and release up to: ten green, one olive ridley, and three loggerhead sea turtles taken in power plant entrainments; four green, one olive ridley, one loggerhead, and two leatherback...

  18. 75 FR 60734 - Endangered Species; File No. 13599-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-01

    ... Lab and used to support law enforcement actions, research studies (primarily genetics), and outreach.... ACTION: Notice; issuance of permit modification. SUMMARY: Notice is hereby given that National Ocean..., Charleston, SC 29412, has been issued a modification to scientific research Permit No. 13599. ADDRESSES: The...

  19. 75 FR 6184 - Endangered Species; File No. 14754

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ... form for a permit to import and take shortnose sturgeon (Acipenser brevirostrum) early life stages (ELS..., to conduct research on Atlantic and shortnose sturgeon. Dr. Wirgin is requesting to conduct a study to determine if early life-stages of Atlantic and shortnose sturgeon are sensitive to PCB...

  20. 75 FR 21601 - Endangered Species; File No. 14604

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-26

    ... blood samples taken for analyses. Another subset of 20 adults per year will be included in hydroacoustic... seasonal spawning activity with gear such as artificial substrate, D-frame ichthyoplankton net, and/or...

  1. Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

    PubMed Central

    Nissen, Silke; Park, Doyoung; Sanford, Robert A.

    2016-01-01

    ABSTRACT Bacteria capable of reduction of nitrous oxide (N2O) to N2 separate into clade I and clade II organisms on the basis of nos operon structures and nosZ sequence features. To explore the possible ecological consequences of distinct nos clusters, the growth of bacterial isolates with either clade I (Pseudomonas stutzeri strain DCP-Ps1, Shewanella loihica strain PV-4) or clade II (Dechloromonas aromatica strain RCB, Anaeromyxobacter dehalogenans strain 2CP-C) nosZ with N2O was examined. Growth curves did not reveal trends distinguishing the clade I and clade II organisms tested; however, the growth yields of clade II organisms exceeded those of clade I organisms by 1.5- to 1.8-fold. Further, whole-cell half-saturation constants (Kss) for N2O distinguished clade I from clade II organisms. The apparent Ks values of 0.324 ± 0.078 μM for D. aromatica and 1.34 ± 0.35 μM for A. dehalogenans were significantly lower than the values measured for P. stutzeri (35.5 ± 9.3 μM) and S. loihica (7.07 ± 1.13 μM). Genome sequencing demonstrated that Dechloromonas denitrificans possessed a clade II nosZ gene, and a measured Ks of 1.01 ± 0.18 μM for N2O was consistent with the values determined for the other clade II organisms tested. These observations provide a plausible mechanistic basis for why the relative activity of bacteria with clade I nos operons compared to that of bacteria with clade II nos operons may control N2O emissions and determine a soil's N2O sink capacity. IMPORTANCE Anthropogenic activities, in particular fertilizer application for agricultural production, increase N2O emissions to the atmosphere. N2O is a strong greenhouse gas with ozone destruction potential, and there is concern that nitrogen may become the major driver of climate change. Microbial N2O reductase (NosZ) catalyzes N2O reduction to environmentally benign dinitrogen gas and represents the major N2O sink process. The observation that bacterial groups with clade I nosZ versus those

  2. VizieR Online Data Catalog: Namelists of variable stars Nos.67-73 (Khopolov+, 1985-97)

    NASA Astrophysics Data System (ADS)

    Kholopov, P. N.; Samus, N. N.; Kazarovets, E. V.; Perova, N. B.

    1997-09-01

    We present a computer-readable version of six Name-Lists of Variable Stars (Nos.67-72) published in the system introduced during the preparation of the 4th GCVS edition. For printed versions of these Name-Lists see IBVS No.2681,1985; No.3058,1987; No.3323,1989; No.3530,1990; No.3840,1993; No.4140,1995. The main body of the 4th GCVS edition is already available in computer-readable form (see , , ). There are 2735 stars in the Name-Lists Nos.67-72 (file "nl.dat"). The variables are arranged in the order of their names inside constellations. One star of the 67th Name-List (V1810 Cyg=No.67247) turned out to be non-existing, it has been excluded from the table. The identification table (file "ident.dat") and the list of references (file "ref.dat") follow. The total number of designated variable stars has now reached 31193. In the new computer-readable version 1.1 of the GCVS, vol. IV (catalog ), we now present identifications also for name-list stars. We make no special attempt to present the material in a completely homogeneous form. There happened minor style evolution from the 67th to the 70th Name-List: particularly, the 67th Name-List normally refers in the identification table only to the catalogues cited in the papers mentioned in the references in the files "nl.dat" and "ident.dat", and to the 70th Name-List it became a common practice to give more catalogue identifications. From the Name-List No.70, there is no completeness in identifications with the old catalogues of suspected variables (CSV), in many cases we consider the NSV identification sufficient. We also do not try to update the information of the older Name-Lists, we only put the information together and correct known mistakes. The corrections to the Name-Lists have been taken into account. It is necessary to reproduce here two remarks from forewords of the printed Name-Lists: Name-List No.69. In the case of the variable stars in the open cluster NGC 3766 (V843-V849 Cen

  3. 77 FR 48511 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-14

    ...: New York Independent System Operator, Inc. Description: NYISO Compliance Filing--Buyer-side Market... qualifying facilities filings can be found at: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf ....

  4. Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus).

    PubMed

    Yadav, Vijay Pratap; Dangi, Satyaveer Singh; Chouhan, Vikrant Singh; Gupta, Mahesh; Dangi, Saroj K; Singh, Gyanendra; Maurya, Vijay Prakash; Kumar, Puneet; Sarkar, Mihir

    2016-03-01

    Approximately 50 genes other than heat shock protein (HSP) expression changes during thermal stress. These genes like nitric oxide synthase (NOS) need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals. So, the present study was undertaken to demonstrate the expressions of inducible form type II NOS (iNOS), endothelial type III NOS (eNOS), constitutively expressed enzyme NOS (cNOS), HSP70, and HSP90 in peripheral blood mononuclear cells (PBMCs) during different seasons in Barbari goats. Real-time polymerase chain reaction, western blot, and immunocytochemistry were applied to investigate messenger RNA (mRNA) expression, protein expression, and immunolocalization of examined factors. The mRNA and protein expressions of iNOS, eNOS, cNOS, HSP70, and HSP90 were significantly higher (P < 0.05) during peak summer, and iNOS and eNOS expressions were also observed to be significantly higher (P < 0.05) during peak winter season as compared with moderate season. The iNOS, eNOS, cNOS, HSP70, and HSP90 were mainly localized in plasma membrane and cytoplasm of PBMCs. To conclude, data generated in the present study indicate the possible involvement of the NOS family genes in amelioration of thermal stress so as to maintain cellular integrity and homeostasis in goats.

  5. Expression analysis of NOS family and HSP genes during thermal stress in goat ( Capra hircus)

    NASA Astrophysics Data System (ADS)

    Yadav, Vijay Pratap; Dangi, Satyaveer Singh; Chouhan, Vikrant Singh; Gupta, Mahesh; Dangi, Saroj K.; Singh, Gyanendra; Maurya, Vijay Prakash; Kumar, Puneet; Sarkar, Mihir

    2016-03-01

    Approximately 50 genes other than heat shock protein (HSP) expression changes during thermal stress. These genes like nitric oxide synthase (NOS) need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals. So, the present study was undertaken to demonstrate the expressions of inducible form type II NOS (iNOS), endothelial type III NOS (eNOS), constitutively expressed enzyme NOS (cNOS), HSP70, and HSP90 in peripheral blood mononuclear cells (PBMCs) during different seasons in Barbari goats. Real-time polymerase chain reaction, western blot, and immunocytochemistry were applied to investigate messenger RNA (mRNA) expression, protein expression, and immunolocalization of examined factors. The mRNA and protein expressions of iNOS, eNOS, cNOS, HSP70, and HSP90 were significantly higher ( P < 0.05) during peak summer, and iNOS and eNOS expressions were also observed to be significantly higher ( P < 0.05) during peak winter season as compared with moderate season. The iNOS, eNOS, cNOS, HSP70, and HSP90 were mainly localized in plasma membrane and cytoplasm of PBMCs. To conclude, data generated in the present study indicate the possible involvement of the NOS family genes in amelioration of thermal stress so as to maintain cellular integrity and homeostasis in goats.

  6. Pedagogical Reflections by Secondary Science Teachers at Different NOS Implementation Levels

    NASA Astrophysics Data System (ADS)

    Herman, Benjamin C.; Clough, Michael P.; Olson, Joanne K.

    2017-02-01

    This study investigated what 13 secondary science teachers at various nature of science (NOS) instruction implementation levels talked about when they reflected on their teaching. We then determined if differences exist in the quality of those reflections between high, medium, and low NOS implementers. This study sought to answer the following questions: (1) What do teachers talk about when asked general questions about their pedagogy and NOS pedagogy and (2) what qualitative differences, if any, exist within variables across teachers of varying NOS implementation levels? Evidence derived from these teachers' reflections indicated that self-efficacy and perceptions of general importance for NOS instruction were poor indicators of NOS implementation. However, several factors were associated with the extent that these teachers implemented NOS instruction, including the utility value they hold for NOS teaching, considerations of how people learn, understanding of NOS pedagogy, and their ability to accurately and deeply self-reflect about teaching. Notably, those teachers who effectively implemented the NOS at higher levels value NOS instruction for reasons that transcend immediate instructional objectives. That is, they value teaching NOS for achieving compelling ends realized long after formal schooling (e.g., lifelong socioscientific decision-making for civic reasons), and they deeply reflect about how to teach NOS by drawing from research about how people learn. Low NOS implementers' simplistic notions and reflections about teaching and learning appeared to be impeding factors to accurate and consistent NOS implementation. This study has implications for science teacher education efforts that promote NOS instruction.

  7. Pedagogical Reflections by Secondary Science Teachers at Different NOS Implementation Levels

    NASA Astrophysics Data System (ADS)

    Herman, Benjamin C.; Clough, Michael P.; Olson, Joanne K.

    2015-10-01

    This study investigated what 13 secondary science teachers at various nature of science (NOS) instruction implementation levels talked about when they reflected on their teaching. We then determined if differences exist in the quality of those reflections between high, medium, and low NOS implementers. This study sought to answer the following questions: (1) What do teachers talk about when asked general questions about their pedagogy and NOS pedagogy and (2) what qualitative differences, if any, exist within variables across teachers of varying NOS implementation levels? Evidence derived from these teachers' reflections indicated that self-efficacy and perceptions of general importance for NOS instruction were poor indicators of NOS implementation. However, several factors were associated with the extent that these teachers implemented NOS instruction, including the utility value they hold for NOS teaching, considerations of how people learn, understanding of NOS pedagogy, and their ability to accurately and deeply self-reflect about teaching. Notably, those teachers who effectively implemented the NOS at higher levels value NOS instruction for reasons that transcend immediate instructional objectives. That is, they value teaching NOS for achieving compelling ends realized long after formal schooling (e.g., lifelong socioscientific decision-making for civic reasons), and they deeply reflect about how to teach NOS by drawing from research about how people learn. Low NOS implementers' simplistic notions and reflections about teaching and learning appeared to be impeding factors to accurate and consistent NOS implementation. This study has implications for science teacher education efforts that promote NOS instruction.

  8. Modulating DDAH/NOS Pathway to Discover Vasoprotective Insulin Sensitizers

    PubMed Central

    Lai, Li; Ghebremariam, Yohannes T.

    2016-01-01

    Insulin resistance syndrome (IRS) is a configuration of cardiovascular risk factors involved in the development of metabolic disorders including type 2 diabetes mellitus. In addition to diet, age, socioeconomic, and environmental factors, genetic factors that impair insulin signaling are centrally involved in the development and exacerbation of IRS. Genetic and pharmacological studies have demonstrated that the nitric oxide (NO) synthase (NOS) genes are critically involved in the regulation of insulin-mediated glucose disposal. The generation of NO by the NOS enzymes is known to contribute to vascular homeostasis including insulin-mediated skeletal muscle vasodilation and insulin sensitivity. By contrast, excessive inhibition of NOS enzymes by exogenous or endogenous factors is associated with insulin resistance (IR). Asymmetric dimethylarginine (ADMA) is an endogenous molecule that competitively inhibits all the NOS enzymes and contributes to metabolic perturbations including IR. The concentration of ADMA in plasma and tissue is enzymatically regulated by dimethylarginine dimethylaminohydrolase (DDAH), a widely expressed enzyme in the cardiovascular system. In preclinical studies, overexpression of DDAH has been shown to reduce ADMA levels, improve vascular compliance, and increase insulin sensitivity. This review discusses the feasibility of the NOS/DDAH pathway as a novel target to develop vasoprotective insulin sensitizers. PMID:26770984

  9. 75 FR 42435 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ... and CBR Baseline Filing to be effective 7/14/2010. Filed Date: 07/14/2010. Accession Number: 20100714... filing per 35.12: MBR and CBR Baseline Filing to be effective 7/14/ 2010. Filed Date: 07/14/2010... filing per 35.12: MBR and CBR Baseline Filing to be effective 7/14/2010. Filed Date: 07/14/2010...

  10. Tumor-expressed iNOS controls induction of functional myeloid derived suppressor cells (MDSC) through modulation of VEGF release1

    PubMed Central

    Jayaraman, Padmini; Parikh, Falguni; Lopez-Rivera, Esther; Hailemichael, Yared; Clark, Amelia; Ma, Ge; Cannan, David; Ramacher, Marcel; Kato, Masashi; Overwijk, Willem W.; Chen, Shu-Hsia; Umansky, Viktor Y.; Sikora, Andrew G.

    2012-01-01

    Inducible nitric oxide synthase (iNOS) is a hallmark of chronic inflammation which is also overexpressed in melanoma and other cancers. While iNOS is a known effector of myeloid-derived suppressor cell (MDSC)-mediated immunosuppression, its pivotal position at the interface of inflammation and cancer also makes it an attractive candidate regulator of MDSC recruitment. We hypothesized that tumor-expressed iNOS controls MDSC accumulation and acquisition of suppressive activity in melanoma. CD11b+Gr1+ MDSC derived from mouse bone marrow cells cultured in the presence of MT-RET-1 mouse melanoma cells or conditioned supernatants expressed STAT3 and reactive oxygen species (ROS) and efficiently suppressed T cell proliferation. Inhibition of tumor-expressed iNOS with the small molecule inhibitor L-NIL blocked accumulation of STAT3/ROS-expressing MDSC, and abolished their suppressive function. Experiments with VEGF-depleting antibody and recombinant VEGF identified a key role for VEGF in the iNOS-dependent induction of MDSC. These findings were further validated in mice bearing transplantable MT-RET-1 melanoma, where L-NIL normalized elevated serum VEGF levels; downregulated activated STAT3 and ROS production in MDSC; and reversed tumor-mediated immunosuppression. These beneficial effects were not observed in iNOS “knockout” mice, suggesting L-NIL acts primarily on tumor-rather than host-expressed iNOS to regulate MDSC function. A significant decrease in tumor growth and a trend towards increased tumor-infiltrating CD8+ T cells was also observed in MT-RET transgenic mice bearing spontaneous tumors. These data suggest a critical role for tumor-expressed iNOS in the recruitment and induction of functional MDSC by modulation of tumor VEGF secretion and upregulation of STAT3 and ROS in MDSC. PMID:22529296

  11. Usage analysis of user files in UNIX

    NASA Technical Reports Server (NTRS)

    Devarakonda, Murthy V.; Iyer, Ravishankar K.

    1987-01-01

    Presented is a user-oriented analysis of short term file usage in a 4.2 BSD UNIX environment. The key aspect of this analysis is a characterization of users and files, which is a departure from the traditional approach of analyzing file references. Two characterization measures are employed: accesses-per-byte (combining fraction of a file referenced and number of references) and file size. This new approach is shown to distinguish differences in files as well as users, which cam be used in efficient file system design, and in creating realistic test workloads for simulations. A multi-stage gamma distribution is shown to closely model the file usage measures. Even though overall file sharing is small, some files belonging to a bulletin board system are accessed by many users, simultaneously and otherwise. Over 50% of users referenced files owned by other users, and over 80% of all files were involved in such references. Based on the differences in files and users, suggestions to improve the system performance were also made.

  12. Gamma-decanolactone inhibits iNOS and TNF-alpha production by lipopolysaccharide-activated microglia in N9 cells.

    PubMed

    Pflüger, Pricila; Viau, Cassiana Macagnan; Coelho, Vanessa Rodrigues; Berwig, Natália Alice; Staub, Renata Bartolomeu; Pereira, Patrícia; Saffi, Jenifer

    2016-06-05

    Activated microglia that produce reactive nitrogen species (RNS), inflammatory factors, reactive oxygen species (ROS), and other neurovirulent factors may lead to the development of neurodegenerative diseases. Certain compounds can inhibit the activation of microglia. However, these mechanisms remain unclear. In the present study, we investigated the inhibitory effect of Gamma-decanolactone (GD) on the production of reactive oxygen species and inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS) - stimulated N9 murine microglial cells through the p38 MAPK signaling pathway. The results showed that GD attenuated the activation of N9 cells and inhibited intracellular reactive oxygen species and the expression of iNOS and TNF-α induced by LPS in the cells. In addition, GD blocked the phosphorylation of p38 and inhibited cleaved caspase-9 and DNA damage. These data indicate that GD has therapeutic potential for the treatment of neurodegenerative diseases, and that it exerts its effects by inhibiting inflammation.

  13. An isolated SNM model for high-stability multi-port register file in 65 nm CMOS

    NASA Astrophysics Data System (ADS)

    Zhang, Yuejun; Wang, Pengjun; Li, Gang

    2017-09-01

    In modern microprocessors, the multi-port register file is one of the key modules which provides fast and multiple data access for instructions. As the number of access ports in register files increases, stability becomes a key issue due to the voltage fluctuation on bit lines. We propose to apply an isolated inverter to address the voltage fluctuation. To assess the register stability, we derive a closed-form expression of static noise margin (SNM) for our register file. The proposed SNM model can be used as a guideline to predict the impact of several register parameters on the stability and optimize register file designs. To validate the proposed SNM model, we fabricated a test chip of two-write-four-read (2W4R) 1024 bits register file in a TSMC 65 nm low-power CMOS technology. The experimental result shows that the stability of our register file cells with an isolated inverter improve the conventional cells by approximately 2.4 times. Also, the supply voltage causes a fluctuation of SNM of about 65%, while temperature and transistor mismatch cause a fluctuation of SNM of about 20%. Project supported by the National Natural Science Foundation of China (Nos, 61404076, 61474068), the Zhejiang Provincial Natural Science Foundation of China (No. LQ14F040001), the S&T Plan of Zhejiang Provincial Science and Technology Department (No. 2015C31010), the China Spark Program (No. 2015GA701053), the Ningbo Natural Science Foundation (Nos. 2014A610148, 2015A610107), and the K. C. Wong Magna Fund in Ningbo University, China.

  14. 29 CFR 24.103 - Filing of retaliation complaint.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... complaint may be filed in any language. (c) Place of Filing. The complaint should be filed with the OSHA...://www.osha.gov. (d) Time for Filing. (1) Except as provided in paragraph (d)(2) of this section, within... considered the date of filing. The time for filing a complaint may be tolled for reasons warranted by...

  15. 29 CFR 24.103 - Filing of retaliation complaint.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... complaint may be filed in any language. (c) Place of Filing. The complaint should be filed with the OSHA...://www.osha.gov. (d) Time for Filing. (1) Except as provided in paragraph (d)(2) of this section, within... considered the date of filing. The time for filing a complaint may be tolled for reasons warranted by...

  16. 29 CFR 24.103 - Filing of retaliation complaint.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... complaint may be filed in any language. (c) Place of Filing. The complaint should be filed with the OSHA...://www.osha.gov. (d) Time for Filing. (1) Except as provided in paragraph (d)(2) of this section, within... considered the date of filing. The time for filing a complaint may be tolled for reasons warranted by...

  17. 12 CFR 1780.9 - Filing of papers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 7 2011-01-01 2011-01-01 false Filing of papers. 1780.9 Section 1780.9 Banks... papers. (a) Filing. Any papers required to be filed shall be addressed to the presiding officer and filed... Director or the presiding officer. All papers filed by electronic media shall also concurrently be filed in...

  18. 78 FR 2979 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... Biomass Power Ltd. Description: MBR Deficiency Filing to be effective 10/29/2012. Filed Date: 1/7/13...: DTE Stoneman, LLC. Description: MBR Deficiency Filing to be effective 10/29/2012. Filed Date: 1/7/13...: DTE Pontiac North, LLC. Description: MBR Deficiency Filing to be effective 10/29/2012. Filed Date: 1/7...

  19. 76 FR 59673 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-27

    ... Services, LLC submits tariff filing per 35.1: Major Energy Electric Services, LLC MBR Re-file to be.... Description: Respond Power, LLC submits tariff filing per 35.1: Respond Power, LLC MBR Re-file to be effective...., submits tariff filing per 35.1: Alternate Power Source, Inc. MBR Re-file to be effective 9/16/ 2011 under...

  20. 77 FR 35371 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-13

    .... Description: MBR Filing to be effective 10/1/2012. Filed Date: 6/5/12. Accession Number: 20120605-5113.... Description: MBR Filing to be effective 10/1/2012. Filed Date: 6/5/12. Accession Number: 20120605-5115.... Description: MBR Filing to be effective 10/1/2012. Filed Date: 6/5/12. Accession Number: 20120605-5116...

  1. 76 FR 63291 - Combined Notice Of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... II,LLC. submits tariff filing per 35: MBR Tariff to be effective 9/23/2011. Filed Date: 09/23/2011... Electric Development Corp. submits tariff filing per 35: MBR Tariff to be effective 9/23/2011. Filed Date... Eastern Generating Corp. submits tariff filing per 35: MBR Tariff to be effective 9/23/2011. Filed Date...

  2. 75 FR 53289 - Combined Notice of Filings No. 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... Electric Corporation submits tariff filing per 35.12: RG&E MBR Baseline eTariff Filing to be effective 8....P. submits tariff filing per 35.12: Covanta Delaware Valley MBR Baseline eTariff Filing to be.... Description: Covanta Essex Company submits tariff filing per 35.12: Covanta Essex MBR Baseline eTariff Filing...

  3. 12 CFR 1780.9 - Filing of papers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Filing of papers. 1780.9 Section 1780.9 Banks... papers. (a) Filing. Any papers required to be filed shall be addressed to the presiding officer and filed... Director or the presiding officer. All papers filed by electronic media shall also concurrently be filed...

  4. 75 FR 12540 - Electronic Tariff Filings; Notice of Technical Conference

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... Energy Regulatory Commission Electronic Tariff Filings; Notice of Technical Conference March 9, 2010. As... electronic tariff filing's data elements is the Type of Filing Code. \\1\\ Electronic Tariff Filings, Order No... addressed the tariff filing definitions used for electronic filings and the attachments that are required...

  5. Brown Norway rats show impaired nNOS-mediated information transfer in renal autoregulation.

    PubMed

    Wang, Xuemei; Cupples, William A

    2009-01-01

    Nonselective inhibition of NO synthase (NOS) augments myogenic autoregulation of renal blood flow (RBF) and profoundly reduces RBF. Previously in Wistar rats, we showed that augmented autoregulation, but not vasoconstriction, is duplicated by intrarenal inhibition of neuronal NOS (nNOS), whereas intrarenal inhibition of inducible NOS (iNOS) has no effect on RBF or on RBF dynamics. Thus macula densa nNOS transfers information from tubuloglomerular feedback to the afferent arteriole. This information flow requires that macula densa nNOS can sufficiently alter ambient NO concentration, that is, that endothelial NOS (eNOS) and iNOS do not alter local NO concentration. Because the Brown Norway rat often shows exaggerated responses to NOS inhibition and has peculiarities of renal autoregulation that are related to NO, we used this strain to study systemic and renal vascular responses to NOS inhibition. The first experiment showed transient blood pressure reduction by bolus i.v. acetylcholine that was dose-dependent in both strains and substantially prolonged in Brown Norway rats. The depressor response decayed more rapidly after nonselective NOS inhibition and the difference between strains was lost, indicating a greater activity of eNOS in Brown Norway rats. In Brown Norway rats, selective inhibition of iNOS reduced RBF (-16% +/- 7%) and augmented myogenic autoregulation, whereas nNOS inhibition reduced RBF (-25% +/- 4%) and did not augment myogenic autoregulation. The significant responses to intrarenal iNOS inhibition, the reduced modulation of autoregulation by nNOS inhibition, and the enhanced endothelial depressor response suggest that physiological signalling by NO within the kidney is impaired in Brown Norway rats because of irrelevant or inappropriate input of NO by eNOS and iNOS.

  6. OTS: a program for converting Noldus Observer data files to SDIS files.

    PubMed

    Bakeman, R; Quera, V

    2000-02-01

    A program for converting Noldus Observer data files (ODF) to sequential data interchange standard (SDIS) files is described. Observer users who convert their data files can then take advantage of various flexible and powerful data modification and computational procedures available in the Generalized Sequential Querier, a program that assumes SDIS-formatted files.

  7. Exploring Elementary Science Methods Course Contexts to Improve Preservice Teachers' NOS of Science Conceptions and Understandings of NOS Teaching Strategies

    ERIC Educational Resources Information Center

    Akerson, Valarie L.; Weiland, Ingrid; Rogers, Meredith Park; Pongsanon, Khemmawaddee; Bilican, Kader

    2014-01-01

    We explored adaptations to an elementary science methods course to determine how varied contexts could improve elementary preservice teachers' conceptions of NOS as well as their ideas for teaching NOS to elementary students. The contexts were (a) NOS Theme in which the course focused on the teaching of science through the consistent teaching…

  8. Exploring Elementary Science Methods Course Contexts to Improve Preservice Teachers' NOS of Science Conceptions and Understandings of NOS Teaching Strategies

    ERIC Educational Resources Information Center

    Akerson, Valarie L.; Weiland, Ingrid; Rogers, Meredith Park; Pongsanon, Khemmawaddee; Bilican, Kader

    2014-01-01

    We explored adaptations to an elementary science methods course to determine how varied contexts could improve elementary preservice teachers' conceptions of NOS as well as their ideas for teaching NOS to elementary students. The contexts were (a) NOS Theme in which the course focused on the teaching of science through the consistent teaching…

  9. 27 CFR 26.275 - Filing.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... TREASURY LIQUORS LIQUORS AND ARTICLES FROM PUERTO RICO AND THE VIRGIN ISLANDS Records and Reports of Liquors From the Virgin Islands Filing and Retention of Records and Reports § 26.275 Filing. (a) All...

  10. 27 CFR 26.275 - Filing.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... TREASURY LIQUORS LIQUORS AND ARTICLES FROM PUERTO RICO AND THE VIRGIN ISLANDS Records and Reports of Liquors From the Virgin Islands Filing and Retention of Records and Reports § 26.275 Filing. (a) All...

  11. 76 FR 12096 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    .... Description: Southern Star Central Gas Pipeline, Inc. submits tariff filing per 154.204: Elk City Tariff Sheet... link to log on and submit the intervention or protests. Persons unable to file electronically...

  12. Fail-over file transfer process

    NASA Technical Reports Server (NTRS)

    Semancik, Susan K. (Inventor); Conger, Annette M. (Inventor)

    2005-01-01

    The present invention provides a fail-over file transfer process to handle data file transfer when the transfer is unsuccessful in order to avoid unnecessary network congestion and enhance reliability in an automated data file transfer system. If a file cannot be delivered after attempting to send the file to a receiver up to a preset number of times, and the receiver has indicated the availability of other backup receiving locations, then the file delivery is automatically attempted to one of the backup receiving locations up to the preset number of times. Failure of the file transfer to one of the backup receiving locations results in a failure notification being sent to the receiver, and the receiver may retrieve the file from the location indicated in the failure notification when ready.

  13. 75 FR 66749 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-29

    .... Docket Numbers: RP11-1420-000. Applicants: ETC Tiger Pipeline, LLC. Description: ETC Tiger Pipeline, LLC submits tariff filing per 154.203: Tiger NAESB Version 1.9 Compliance Filing to be effective 12/ 1/2010...

  14. 75 FR 67356 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-02

    .... Docket Numbers: RP11-1420-000. Applicants: ETC Tiger Pipeline, LLC. Description: ETC Tiger Pipeline, LLC submits tariff filing per 154.203: Tiger NAESB Version 1.9 Compliance Filing to be effective 12/ 1/2010...

  15. 76 FR 12962 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-09

    ... Working Agreement. Parties: CMA CGM S.A. and Mediterranean Shipping Company S.A. Filing Party: Draughn..., CMA CGM S.A., and Mediterranean Shipping Company S.A. Filing Party: Wayne R. Rohde, Esq.; Cozen O...

  16. 76 FR 11473 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... Space Charter Agreement. Parties: A.P. Moeller-Maersk A/S and Mediterranean Shipping Company S.A. Filing.... Parties: Hapag-Lloyd AG; and Mediterranean Shipping Co. S.A. (``MSC''). Filing Party: Wayne Rohde, Esq...

  17. 77 FR 14358 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-09

    ...: RP12-469-000. Applicants: Sea Robin Pipeline Company, LLC. Description: Hurricane Surcharge Filing on 3...: RP12-313-001. Applicants: Sea Robin Pipeline Company, LLC. Description: Hurricane Compliance Filing...

  18. 78 FR 30914 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ... Sharing Profits from Base Gas Sales with Customers of Columbia Gas Transmission, LLC. Filed Date: 5/2/13...: Millennium Pipeline Company, LLC. Description: NAESB 2.0 Waiver Removal to be effective 12/1/2012. Filed Date...

  19. 78 FR 64490 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-29

    ...: Negotiated Rate--Tenaska LPS RO to be effective 10/16/ 2013. Filed Date: 10/16/13. Accession Number: 20131016... Company of America. Description: Tenaska LPS RO Negotiated Rate to be effective 10/16/ 2013. Filed...

  20. 78 FR 76624 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-18

    ... & Logistic GmbH & Co. KG; Industrial Maritime Carriers LLC; Seaboard Marine, Ltd. Filing Party: Wade S...H & Cie. KG. Filing Party: Wade S. Hooker, Esq.; 211 Central Park W; New York, NY 10024. Synopsis...

  1. 77 FR 60433 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-03

    ... Express, Inc. and Great White Fleet Liner Services Ltd. Filing Party: Wade S. Hooker, Esq., 211 Central... White Fleet Liner Services Ltd. and Crowley Latin American Services, LLC. Filing Party: Wade S. Hooker...

  2. 77 FR 506 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-05

    ... Express, LLC. Filing Party: Wade S. Hooker, Esq.; 211 Central Park W; New York, NY 10024. Synopsis: The... Interoceanica, S.A and Hamburg Sudamerikanische Dampfschiffahrts-Gesellschaft KG. Filing Party: Wade S. Hooker...

  3. 77 FR 63832 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ... Autoliners AS and Nippon Yusen Kaisha (``NYK''). Filing Party: Wayne R. Rohde, Esq.; Cozen O'Connor; 1627 I...-Lloyd AG. Filing Party: Wayne R. Rohde, Esq.; Cozen O'Connor; 1627 I Street NW., Suite 1100;...

  4. 77 FR 35384 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-13

    ...; and Mediterranean Shipping Company S.A. Filing Party: Wayne R. Rohde, Esq.; Cozen O'Connor; 1627 I.... Ltd. Filing Party: Wayne R. Rohde, Esquire, Cozen O'Connor, 1627 I Street NW., Suite 1100;...

  5. 78 FR 49515 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-14

    .... Parties: Nippon Yusen Kaisha, and Hanjin Shipping Co. Ltd. Filing Parties: Wayne R. Rohde, Esq.; Cozen O... Industria Armamento Meridionale S.p.A. (Inarme). Filing Party: Brooke F. Shapiro; Winston & Strawn LLP; 200...

  6. 76 FR 55375 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ... 154.204: Enbridge 34685 to Texla 39017 Capacity Release Negotiated Rate Agreement Filing to be... Company, LP. Description: Gulf South Pipeline Company, LP submits tariff filing per 154.204: Enbridge...

  7. 75 FR 79362 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-20

    ...-000. Applicants: Bobcat Gas Storage. Description: Bobcat Gas Storage submits tariff filing per 154.204...-000. Applicants: Bobcat Gas Storage. Description: Bobcat Gas Storage submits tariff filing per...

  8. 75 FR 57754 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-22

    ... 22, 2010. Docket Numbers: RP10-1289-000. Applicants: Bobcat Gas Storage. ] Description: Bobcat Gas Storage submits tariff filing per 154.203: Bobcat Baseline Tariff Filing to be effective 9/10/2010....

  9. 75 FR 39229 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-08

    ..., July 06, 2010. Docket Numbers: RP10-875-000. Applicants: NGO Transmission, Inc. Description: NGO Transmission, Inc. submits tariff filing per 154.203: NGO Transmission--Baseline eTariff Filing to be...

  10. 78 FR 58533 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-24

    ... received the following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings... Docket Numbers: CP13-548-000 Applicants: Montana-Dakota Utilities Co. Description: Application for...

  11. 75 FR 24702 - Notice of Agreements Filed

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-05

    ... Line, Ltd. Filing Party: Wayne R. Rohde, Esq.; Sher & Blackwell LLP; 1850 M Street, NW.; Suite 900... Authority. Filing Parties: Greg Lovelace, Director Cargo & Cruise Marketing; Tampa Port Authority; 1101...

  12. 12 CFR 303.221 - Filing procedures.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... PROCEDURES Section 19 of the FDI Act (Consent to Service of Persons Convicted of Certain Criminal Offenses) § 303.221 Filing procedures. (a) Where to file. An application under section 19 of the FDI Act shall...

  13. 12 CFR 303.221 - Filing procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... PROCEDURES Section 19 of the FDI Act (Consent to Service of Persons Convicted of Certain Criminal Offenses) § 303.221 Filing procedures. (a) Where to file. An application under section 19 of the FDI Act shall...

  14. 12 CFR 303.221 - Filing procedures.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... PROCEDURES Section 19 of the FDI Act (Consent to Service of Persons Convicted of Certain Criminal Offenses) § 303.221 Filing procedures. (a) Where to file. An application under section 19 of the FDI Act shall...

  15. 12 CFR 303.221 - Filing procedures.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... PROCEDURES Section 19 of the FDI Act (Consent to Service of Persons Convicted of Certain Criminal Offenses) § 303.221 Filing procedures. (a) Where to file. An application under section 19 of the FDI Act shall...

  16. 47 CFR 1.1113 - Filing locations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Schedule of Statutory Charges and Procedures for Payment § 1.1113 Filing locations. (a) Except as noted in... returned to the applicant or filer. (c) Fees for applications and other filings pertaining to the Wireless...

  17. 47 CFR 1.1113 - Filing locations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Schedule of Statutory Charges and Procedures for Payment § 1.1113 Filing locations. (a) Except as noted in... returned to the applicant or filer. (c) Fees for applications and other filings pertaining to the Wireless...

  18. 78 FR 46935 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-02

    ...: 20130725-5032. Comments Due: 5 p.m. ET 8/6/13. Docket Numbers: RP13-1098-000. Applicants: Bison Pipeline LLC. Description: Bison Pipeline LLC submits tariff filing per 154.203: ACA Filing 2013 to...

  19. 78 FR 35017 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-11

    ... found at: http://www.ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free). For TTY, call (202) 502-8659. Dated: June 4, 2013. Nathaniel J. Davis, Sr., Deputy...

  20. 77 FR 34983 - Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-12

    ... Meridian, Oklahoma (OK) The plat, representing the dependent resurvey and survey in Township 24 ] North... reasons must be filed with the State Director within thirty (30) days after the protest is filed. Stephen...

  1. 75 FR 1764 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-13

    ... Numbers: RP10-271-000. Applicants: Southern LNG Inc. Description: Southern LNG, Inc submits for filing... Interstate Company, Ltd submits for filing and acceptance a firm transportation service agreement with Devon...

  2. 75 FR 61728 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ...: Negative Rate 2010-09-27 MGE to be effective 11/1/2010. Filed Date: 09/27/2010. Accession Number: 20100927... Commission has received the following Natural Gas Pipeline Rate and Refund Report filings: Docket...

  3. National Plan to Expand NOS' Operational Modeling Capabilities

    NASA Astrophysics Data System (ADS)

    Zhang, A.; Burke, P.

    2016-02-01

    Real-time marine forecast products based on numerical ocean models are a powerful tool serving a variety of uses, such as marine navigation planning for commercial port operations, harmful algal bloom formation and tracking, hazard response, and emergency search and rescue. Advances in data acquisition technology, ocean dynamics, numerical schemes and visualization tools have made marine forecasting products more accurate and reliable, and the dissemination of operational model products more timely and user friendly. NOAA's National Ocean Service (NOS) has been developing and maintaining a national network of hydrodynamic operational oceanographic nowcast and forecast modeling systems to support navigational and environmental applications in U.S. coastal and estuarine waters, and the Great Lakes. These operational forecast systems (OFS) provide the maritime community with nowcast and forecast guidance of water levels, currents, water temperature, and salinity for 48 to 120 hours. NOS has collaborated with the National Weather Service (NWS) and the Office of Oceanic and Atmospheric Research (OAR) and extramural partners from academia and the local maritime community to develop and implement the current suite of NOS OFS. NOS currently operates and maintains 15 OFS for the U.S. East and West coasts, the Gulf of Mexico, and the Great Lakes, to cover approximately 35% of the CONUS coast. Over the next 8 to 10 years, NOS intends to provide complete coverage of the continental U.S and establish the necessary national infrastructure to enable other types of forecasts. This presentation will provide an overview of NOS' new regional approach to expand its OFS capabilities and services nationally, a pathway to transition research models and applications to operations, and anticipated challenges to implement this plan.

  4. 33 CFR 148.246 - When is a document considered filed and where should I file it?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... filed and where should I file it? 148.246 Section 148.246 Navigation and Navigable Waters COAST GUARD... Formal Hearings § 148.246 When is a document considered filed and where should I file it? (a) If a document to be filed is submitted by mail, it is considered filed on the date it is postmarked. If a...

  5. 33 CFR 148.246 - When is a document considered filed and where should I file it?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... filed and where should I file it? 148.246 Section 148.246 Navigation and Navigable Waters COAST GUARD... Formal Hearings § 148.246 When is a document considered filed and where should I file it? (a) If a document to be filed is submitted by mail, it is considered filed on the date it is postmarked. If...

  6. 78 FR 23244 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-18

    ....ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free...--RP12-1021 & RP13-584. Filed Date: 4/10/13. Accession Number: 20130410-5105. Comments Due: 5 p.m. ET 4...) to be effective 4/9/2013. Filed Date: 4/9/13. Accession Number: 20130409-5095. Comments Due: 5 p.m...

  7. 77 FR 1927 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-12

    .../docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free). For TTY...: Removal of Expired Agreements to be effective 2/4/ 2012. Filed Date: 1/4/12. Accession Number: 20120104... effective 1/1/2012. Filed Date: 1/4/12. Accession Number: 20120104-5144. Comments Due: 5 p.m. ET 1/17/12...

  8. 78 FR 79432 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings Take notice that the Commission has received the following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket Numbers: RP14-293-000....

  9. 40 CFR 78.4 - Filings.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 16 2010-07-01 2010-07-01 false Filings. 78.4 Section 78.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPEAL PROCEDURES § 78.4 Filings. (a) All original filings made under this part shall be signed by the person making the...

  10. 40 CFR 78.4 - Filings.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 17 2013-07-01 2013-07-01 false Filings. 78.4 Section 78.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPEAL PROCEDURES § 78.4 Filings. (a)(1) All original filings made under this part shall be signed by the person making...

  11. 78 FR 49494 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-14

    .... Comments Due: 5 p.m. ET 8/12/13. Docket Numbers: RP13-1134-000. Applicants: Panther Interstate Pipeline Energy, LLC. Description: Panther Order No. 776 Compliance Filing to be effective 10/1/2013. Filed Date...-000. Applicants: Florida Gas Transmission Company, LLC. Description: ACA Compliance Filing on...

  12. 47 CFR 1.1405 - File numbers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false File numbers. 1.1405 Section 1.1405... Procedures § 1.1405 File numbers. Each complaint which appears to be essentially complete under § 1.1404 will be accepted and assigned a file number. Such assignment is for administrative purposes only and...

  13. 47 CFR 1.911 - Station files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Station files. 1.911 Section 1.911... Applications and Proceedings Application Requirements and Procedures § 1.911 Station files. Applications... maintained by the Commission in ULS. These files constitute the official records for these stations...

  14. 21 CFR 211.198 - Complaint files.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Complaint files. 211.198 Section 211.198 Food and... files. (a) Written procedures describing the handling of all written and oral complaints regarding a...) A written record of each complaint shall be maintained in a file designated for drug...

  15. 37 CFR 1.251 - Unlocatable file.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Unlocatable file. 1.251 Section 1.251 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF... § 1.251 Unlocatable file. (a) In the event that the Office cannot locate the file of an...

  16. 36 CFR 703.20 - File copies.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false File copies. 703.20 Section... Is Not a Party § 703.20 File copies. The Office of the General Counsel will maintain the official file of copies of all demands served on the Library and deciding officials' responses....

  17. 21 CFR 225.115 - Complaint files.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Complaint files. 225.115 Section 225.115 Food and... CURRENT GOOD MANUFACTURING PRACTICE FOR MEDICATED FEEDS Records and Reports § 225.115 Complaint files. (a... feed manufacturer shall maintain on the premises a file which contains the following information:...

  18. 46 CFR 502.161 - Commission's files.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Commission's files. 502.161 Section 502.161 Shipping...; Presiding Officers; Evidence § 502.161 Commission's files. Where any matter contained in a tariff, report, or other document on file with the Commission is offered in evidence, such document need not...

  19. 10 CFR 501.35 - Public file.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Public file. 501.35 Section 501.35 Energy DEPARTMENT OF... Hearings and Conferences During Administrative Proceedings § 501.35 Public file. (a) Contents. The public file shall consist of the rule, order, or petition, with supporting data and supplemental...

  20. 21 CFR 1271.320 - Complaint file.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Complaint file. 1271.320 Section 1271.320 Food and... CELLULAR AND TISSUE-BASED PRODUCTS Current Good Tissue Practice § 1271.320 Complaint file. (a) Procedures... the investigation of complaints as appropriate. (b) Complaint file. You must maintain a record...