The Breed and Sex Effect on the Carcass Size Performance and Meat Quality of Yak in Different Muscles

PubMed Central

Zhang, Li; Sun, Baozhong; Yu, Qunli; Ji, Qiumei; Xie, Peng; Li, Haipeng; Wang, Li; Zhou, Yuchun; Li, Yongpeng; Huang, Caixia; Liu, Xuan

2016-01-01

The carcass size performances and the meat quality of Gannan and Sibu yak were determined using M. supraspinatus (SU), M. longissimus thoracis (LT) and M.quadriceps femoris (QF). It is found that Sibu yak had significantly higher carcass weight (CW) than Gannan yak with difference of nearly 40 kg, as well as significantly higher eye muscle area (EMA), carcass thorax depth (CTD), round perimeter (RP), etc. The carcass performances of steer yak were significantly higher than heifer yak except meat thickness at round (MTR) (p<0.05). The results show that both yak breed and gender had significant effects on carcass performances. It could be seen that the variation of carcass size performances from breeds is as large as from gender (50.22% and 46.25% of total variation, respectively) through principal component analysis (PCA). Sibu yak had significantly higher L*, b*, WBSF, cooking loss and Fat content, while Gannan yak had significantly higher a*, press loss, protein content and moisture (p<0.05). Yak gender and muscle had insignificant effects on meat colour and water holding capacity (p>0.05). The variation of meat quality of yak from breed is up to 59.46% of total variation according to PCA. It is shown that the difference between breeds, for Gannan yak and Sibu yak, plays an important role in carcass size performance and meat quality. PMID:27194931

Seroprevalence and Risk Factors of Fascioliasis in Yaks, Bos grunniens, from Three Counties of Gansu Province, China.

PubMed

Zhang, Xiao-Xuan; Feng, Sheng-Yong; Ma, Jian-Gang; Zheng, Wen-Bin; Yin, Ming-Yang; Qin, Si-Yuan; Zhou, Dong-Hui; Zhao, Quan; Zhu, Xing-Quan

2017-02-01

The aim of this study was to determine the seroprevalence and risk factors of fascioliasis in yaks, Bos grunniens , from 3 counties of Gansu Province in China. A total of 1,584 serum samples, including 974 samples from white yaks from Tianzhu, 464 from black yaks from Maqu, and 146 from black yaks from Luqu County, were collected and analyzed using ELISA to detect IgG antibodies against Fasciola hepatica . The overall F. hepatica seroprevalence was 28.7% (454/1,584), with 29.2% in white yaks (284/974) and 27.9% in black yaks (170/610). The seroprevalence of F. hepatica in yaks from Tianzhu, Luqu, and Maqu was 29.2%, 22.6%, and 29.5%, respectively. Female yaks (30.9%) had higher F. hepatica seroprevalence than male yaks (23.4%). Also, F. hepatica seroprevalence varied by different age group from 24.1% to 33.8%. Further, the seroprevalence ranged from 21.8% to 39.1% over different seasons. Interestingly, the season and age of yaks were associated with F. hepatica infection in yaks in the investigated areas. These findings provided a basis for further studies on this disease in yaks from 3 counties of Gansu Province in northwestern China, which may ultimately support the development of effective control strategies of fascioliasis in these areas.

Y-STR INRA189 polymorphisms in Chinese yak breeds.

PubMed

Ma, Z J; Chen, S M; Sun, Y G; Xi, Y L; Li, R Z; Xu, J T; Lei, C Z

2015-12-29

To further explore Y-STR INRA189 polymorphisms in the yak, and to determine the genetic differences among yak breeds, genotyping analysis of INRA189 in 102 male yak individuals from three yak breeds in Qinghai Province of China was performed. Genotyping revealed the presence of four alleles, with sizes of 149, 155, 157, and 159 bp, respectively. Of these, the 157-bp allele, which was found with the highest frequency in the three yak breeds, was the dominant allele. Interestingly, the 149-bp allele was only detected in the Gaoyuan breed, and the 159-bp allele was only found in the Huanhu and Datong breeds. Only the 157- and 155-bp alleles were found in all three yak breeds. Taking the three yak breeds as a single population, the frequency of these four alleles was 0.0294, 0.0686, 0.8628, and 0.0392, respectively. The average polymorphism information content in the three yak breeds was 0.2379, indicating that the INRA189 was a low polymorphic Y-STR marker in yak.

[Discussion on reduction potential of CH4 emission intensity for early off-take practice of grazing yak].

PubMed

Wang, Shi-Ping; Wilkes, Andreas; Wang, Ya-Yun; Bai, Ling

2014-08-01

The case study preliminarily compared the CH4 reduction potential and CH4 emission intensity of 7 year-old and 4 year-old grazing yak after early off-take practice based on the 2006 IPCC GHG inventory guidelines and under the premise of equal herbage consumption. Our results showed that the total CH4 emission was greater by about 86.3 kg for 2.1 4-year yaks compared with 7 years old yak during their life assuming that their total herbage consumption was the same, because total herbage consumption for a 7-year yak was equal to that of 2.1 4-year yaks. However, CH4 emission per unit body weight (1.374 kg x kg(-1)) for a 7-year yak (i. e. emission intensity) was higher than that of 2.1 4-year yaks (0.973 kg x kg(-1)) because total body weight of 2.1 4-year yaks was higher by 192 kg than that of a 7-year yak. According to CH4 emission intensity, change of the early off-take practice from 7-year to 4-year yak could reduce 77 kg CH4 if producing 192 kg body weight through 2.1 4-year yaks compared with a 7-year yak, i. e. reduction potential was about 1 600 kg CO2 equivalent under the same consuming forage. Therefore, for grassland-based animal husbandry, early off-take practice for grazing animals had a great reduction potential in the intensity of greenhouse gases (GHGs) emissions per unit output rather than total emissions of GHGs.

Toward Understanding the Genetic Basis of Yak Ovary Reproduction: A Characterization and Comparative Analyses of Estrus Ovary Transcriptiome in Yak and Cattle.

PubMed

Lan, Daoliang; Xiong, Xianrong; Huang, Cai; Mipam, Tserang Donko; Li, Jian

2016-01-01

Yaks (Bos grunniens) are endemic species that can adapt well to thin air, cold temperatures, and high altitude. These species can survive in harsh plateau environments and are major source of animal production for local residents, being an important breed in the Qinghai-Tibet Plateau. However, compared with ordinary cattle that live in the plains, yaks generally have lower fertility. Investigating the basic physiological molecular features of yak ovary and identifying the biological events underlying the differences between the ovaries of yak and plain cattle is necessary to understand the specificity of yak reproduction. Therefore, RNA-seq technology was applied to analyze transcriptome data comparatively between the yak and plain cattle estrous ovaries. After deep sequencing, 3,653,032 clean reads with a total of 4,828,772,880 base pairs were obtained from yak ovary library. Alignment analysis showed that 16992 yak genes mapped to the yak genome, among which, 12,731 and 14,631 genes were assigned to Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Furthermore, comparison of yak and cattle ovary transcriptome data revealed that 1307 genes were significantly and differentially expressed between the two libraries, wherein 661 genes were upregulated and 646 genes were downregulated in yak ovary. Functional analysis showed that the differentially expressed genes were involved in various Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. GO annotations indicated that the genes related to "cell adhesion," "hormonal" biological processes, and "calcium ion binding," "cation transmembrane transport" molecular events were significantly active. KEGG pathway analysis showed that the "complement and coagulation cascade" pathway was the most enriched in yak ovary transcriptome data, followed by the "cytochrome P450" related and "ECM-receptor interaction" pathways. Moreover, several novel pathways, such as "circadian rhythm," were significantly enriched despite having no evident associations with the reproductive function. Our findings provide a molecular resource for further investigation of the general molecular mechanism of yak ovary and offer new insights to understand comprehensively the specificity of yak reproduction.

Yak milk fat globules from the Qinghai-Tibetan Plateau: Membrane lipid composition and morphological properties.

PubMed

Luo, Jie; Huang, Ziyu; Liu, Hongna; Zhang, Yan; Ren, Fazheng

2018-04-15

Yak milk fat products constitute the base of Qinghai-Tibetan pastoralists' daily food intake. Despite the great importance of fat in processing and pastoralists' health, studies about yak milk fat are scarce. In this study, the lipid composition and the morphological properties of milk fat globule membranes (MFGMs) of yak milk were investigated. The results demonstrated that the yak milk had a higher cholesterol and sphingomyelin content compared to cow milk. In situ structural investigations performed at 25 °C by confocal microscopy showed the presence of lipid domains in yak MFGM, with a larger number and wider size range compared to cow milk. Moreover, the simultaneous localization of glycosylated molecules and polar lipids indicated that glycosylated molecules could be integrated into the lipid domains in yak MFGM. Different characteristics in yak MFGM could be related to the lipid composition and may affect the functions of yak milk lipids during processing and digestion. Copyright © 2017 Elsevier Ltd. All rights reserved.

A Study on Temperature and Precipitation Variability in Pre-monsoon Period of 2016 in the Dudh Khola River Valley, Manang, Nepal

NASA Astrophysics Data System (ADS)

Kayastha, R.; Kayastha, R. B.; Chand, M. B.; Armstrong, R. L.

2016-12-01

Meteorological data are the key parameter for deeper and better understanding the local to regional climate variability. Temperature and precipitation are highly dependent on elevation and it is foremost important in water resource management. The runoff from glacierized catchments is greatly influenced by the variation in temperature and precipitation. However, inaccessibility limits the hydro-meteorological data observation in high altitudes. In this study, temperature and precipitation data are observed and analyzed from six stations including two weather stations in different elevation ranging from 1926 to 3908 m a.s.l. in the Dudh Khola River basin, a sub basin of Marsyangdi River basin from March to June 2016 (pre-monsoon period). Clear spatial and temporal variability of temperature lapse rate (TLR) is observed which is related to the extent of humid air. The hourly mean TLR shows highly heterogeneous between the different elevations from - 0.72 o C, -0.51 o C, -0.77 o C, -0.68 to +0.42 o C per 100 m and the hourly linear regression of TLR is - 0.54 o C per 100 m. Similarly, vertical precipitation gradients (PG) between Dharapani & Goa, Goa & Yak Kharka, and Yak Kharka & glacier station are 0.040, 0.037 and 0.032 per meter respectively. Horizontal precipitation gradient from lower station to the higher station in a distance of 16 km is 0.0015 mm per meter. The TLR from the recorded period are less than the environmental lapse rate in the Dudh Khola Valley in pre-monsoon season. From this study it can be concluded that hourly and daily lapse rates and PGs can be used to improve the output of the glacio-hydrological and energy balance modelling in glacierized river basin.

The genetics of brown coat color and white spotting in domestic yaks (Bos grunniens).

PubMed

Zhang, M-Q; Xu, X; Luo, S-J

2014-10-01

Domestic yaks (Bos grunniens) exhibit two major coat color variations: a brown vs. wild-type black pigmentation and a white spotting vs. wild-type solid color pattern. The genetic basis for these variations in color and distribution remains largely unknown and may be complicated by a breeding history involving hybridization between yaks and cattle. Here, we investigated 92 domestic yaks from China using a candidate gene approach. Sequence variations in MC1R, PMEL and TYRP1 were surveyed in brown yaks; TYRP1 was unassociated with the coloration and excluded. Recessive mutations from MC1R, or p.Gln34*, p.Met73Leu and possibly p.Arg142Pro, are reported in bovids for the first time and accounted for approximately 40% of the brown yaks in this study. The remaining 60% of brown individuals correlated with a cattle-derived deletion mutation from PMEL (p.Leu18del) in a dominant manner. Degrees of white spotting found in yaks vary from color sidedness and white face, to completely white. After examining the candidate gene KIT, we suggest that color-sided and all-white yaks are caused by the serial translations of KIT (Cs6 or Cs29 ) as reported for cattle. The white-faced phenotype in yaks is associated with the KIT haplotype S(wf) . All KIT mutations underlying the serial phenotypes of white spotting in yaks are identical to those in cattle, indicating that cattle are the likely source of white spotting in yaks. Our results reveal the complex genetic origins of domestic yak coat color as either native in yaks through evolution and domestication or as introduced from cattle through interspecific hybridization. © 2014 Stichting International Foundation for Animal Genetics.

Toward Understanding the Genetic Basis of Yak Ovary Reproduction: A Characterization and Comparative Analyses of Estrus Ovary Transcriptiome in Yak and Cattle

PubMed Central

Huang, Cai; Mipam, Tserang Donko; Li, Jian

2016-01-01

Background Yaks (Bos grunniens) are endemic species that can adapt well to thin air, cold temperatures, and high altitude. These species can survive in harsh plateau environments and are major source of animal production for local residents, being an important breed in the Qinghai–Tibet Plateau. However, compared with ordinary cattle that live in the plains, yaks generally have lower fertility. Investigating the basic physiological molecular features of yak ovary and identifying the biological events underlying the differences between the ovaries of yak and plain cattle is necessary to understand the specificity of yak reproduction. Therefore, RNA-seq technology was applied to analyze transcriptome data comparatively between the yak and plain cattle estrous ovaries. Results After deep sequencing, 3,653,032 clean reads with a total of 4,828,772,880 base pairs were obtained from yak ovary library. Alignment analysis showed that 16992 yak genes mapped to the yak genome, among which, 12,731 and 14,631 genes were assigned to Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Furthermore, comparison of yak and cattle ovary transcriptome data revealed that 1307 genes were significantly and differentially expressed between the two libraries, wherein 661 genes were upregulated and 646 genes were downregulated in yak ovary. Functional analysis showed that the differentially expressed genes were involved in various Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. GO annotations indicated that the genes related to “cell adhesion,” “hormonal” biological processes, and “calcium ion binding,” “cation transmembrane transport” molecular events were significantly active. KEGG pathway analysis showed that the “complement and coagulation cascade” pathway was the most enriched in yak ovary transcriptome data, followed by the “cytochrome P450” related and “ECM–receptor interaction” pathways. Moreover, several novel pathways, such as “circadian rhythm,” were significantly enriched despite having no evident associations with the reproductive function. Conclusion Our findings provide a molecular resource for further investigation of the general molecular mechanism of yak ovary and offer new insights to understand comprehensively the specificity of yak reproduction. PMID:27044040

Parameters of thunderstorm activity and lightning discharges in Central Yakutia from 2009 to 2012

NASA Astrophysics Data System (ADS)

Kozlov, V. I.; Mullayarov, V. A.; Grigorev, Yu. M.; Tarabukina, L. D.

2014-05-01

The results of integrated instrumental observations of thunderstorm activity around Yakutsk at a radius of 400 and 30 km are presented. The seasonal course of thunderstorm activity was found to contain characteristic peaks in the first 10 days of June and the last 10 days of July or early August. The fraction of cloud-to-ground discharges in Central Yakutia is 40-60%, which is consistent with observations in Western Siberia (40-50%). The number of positive discharges to the ground was 8-15% of all cases of discharges to the ground, which is consistent with observations in Germany (17%) and slightly exceeds the observed number in the Caucasus (2.2-8.2%) and United States (4.5%). The thunderstorm activity in Yakutsk is three times higher than in the area around Yakutsk with a radius of 400 km, which can be explained by the fact that the city is a heat island.

Zenith angle distribution of cosmic ray showers measured with the Yakutsk array and its application to the analysis of arrival directions in equatorial coordinates

NASA Astrophysics Data System (ADS)

Ivanov, A. A.

2018-04-01

The Yakutsk array data set in the energy interval (1017,1019) eV is revisited in order to interpret the zenith angle distribution of an extensive air shower event rate of ultra-high-energy cosmic rays. The close relation of the distribution to the attenuation of the main measurable parameter of showers, ρ600, is examined. Measured and expected distributions are used to analyze the arrival directions of cosmic rays on an equatorial map including the energy range below 1018 eV , which was previously avoided due to the reduced trigger efficiency of the array in the range. While the null hypothesis cannot be rejected with data from the Yakutsk array, an upper limit on the fraction of cosmic rays from a separable source in the uniform background is derived as a function of declination and energy.

[Expression levels of Cdc2 and Cdc25A mRNA in cattle, yak, and cattle-yak testis].

PubMed

Dong, Li-Yan; Li, Qi-Fa; Qu, Xu-Guang; Li, Yin-Xia; Li, Xin-Fu; Hu, Hong-Tao; Xie, Zhuang

2009-05-01

The infertility of cattle-yak, which is the hybrid offspring of cattle and yak, is a difficult problem in crossbreeding and improvement of yak. Cdc2 and Cdc25A are the key genes of meiosis. The decline of their expression levels will cause the spermatogenesis failure and lead to infertility. Therefore, this study was conducted to study the relationship between the infertility of cattle-yak and the expression levels of Cdc2/Cdc25A genes. The expression profiles were obtained by RT-PCR. Cdc2 and Cdc25A genes were widely expressed in many tissues, which confirmed their important role in cell division and the progression of cell cycle. Real-time quantitative PCR analysis indicated that the expression levels of Cdc2 and Cdc25A in cattle and yak testis were higher than those in cattle-yak (P<0.05). Therefore, low expression levels of Cdc2 and Cdc25A genes may have a relationship with the infertility of cattle-yak.

Identification and characterization of yak (Bos grunniens) b-Boule gene and its alternative splice variants.

PubMed

Li, Bojiang; Ngo, Sherry; Wu, Wangjun; Xu, Hongtao; Xie, Zhuang; Li, Qifa; Pan, Zengxiang

2014-10-25

Boule is responsible for meiotic arrest of sperms and male sterility during mammalian spermatogenesis. In the present study, we first identified yak b-Boule gene and its two alternative splice variants. The full length coding region of yak b-Boule is 888bp and encodes a 295-amino acid protein with a typical RNA-recognition motif (RRM) and a Deleted in Azoospermia (DAZ) repetitive sequence motif. Two alternative splice variants of yak b-Boule were generated following the consensus "GT-AG" rule and named b-Boule1 (36bp deletion in exon 3) and b-Boule2 (deletion of integral exon 7), respectively. In male yak, b-Boule, b-Boule1 and b-Boule2 were found to be exclusively expressed in the testes at a ratio of 81:0.1:1. Intriguingly, the mRNA expression levels of b-Boule and b-Boule1 in yak testis were significantly higher than those in cattle-yak, although no significant difference was observed for b-Boule2 expression between the yak and cattle-yak. These results suggest that b-Boule gene, which is partially regulated by alternative splicing, may be involved in the process of yak spermatogenesis. Copyright © 2014 Elsevier B.V. All rights reserved.

Transcriptome profile and unique genetic evolution of positively selected genes in yak lungs.

PubMed

Lan, DaoLiang; Xiong, XianRong; Ji, WenHui; Li, Jian; Mipam, Tserang-Donko; Ai, Yi; Chai, ZhiXin

2018-04-01

The yak (Bos grunniens), which is a unique bovine breed that is distributed mainly in the Qinghai-Tibetan Plateau, is considered a good model for studying plateau adaptability in mammals. The lungs are important functional organs that enable animals to adapt to their external environment. However, the genetic mechanism underlying the adaptability of yak lungs to harsh plateau environments remains unknown. To explore the unique evolutionary process and genetic mechanism of yak adaptation to plateau environments, we performed transcriptome sequencing of yak and cattle (Bos taurus) lungs using RNA-Seq technology and a subsequent comparison analysis to identify the positively selected genes in the yak. After deep sequencing, a normal transcriptome profile of yak lung that containing a total of 16,815 expressed genes was obtained, and the characteristics of yak lungs transcriptome was described by functional analysis. Furthermore, Ka/Ks comparison statistics result showed that 39 strong positively selected genes are identified from yak lungs. Further GO and KEGG analysis was conducted for the functional annotation of these genes. The results of this study provide valuable data for further explorations of the unique evolutionary process of high-altitude hypoxia adaptation in yaks in the Tibetan Plateau and the genetic mechanism at the molecular level.

Genome-wide patterns of copy number variation in the Chinese yak genome.

PubMed

Zhang, Xiao; Wang, Kun; Wang, Lizhong; Yang, Yongzhi; Ni, Zhengqiang; Xie, Xiuyue; Shao, Xuemin; Han, Jin; Wan, Dongshi; Qiu, Qiang

2016-05-20

Copy number variation (CNV) represents an important source of genetic divergence that can produce drastic phenotypic differences and may therefore be subject to selection during domestication and environmental adaptation. To investigate the evolutionary dynamics of CNV in the yak genome, we used a read depth approach to detect CNV based on genome resequencing data from 14 wild and 65 domestic yaks and determined CNV regions related to domestication and adaptations to high-altitude. We identified 2,634 CNV regions (CNVRs) comprising a total of 153 megabases (5.7 % of the yak genome) and 3,879 overlapping annotated genes. Comparison between domestic and wild yak populations identified 121 potentially selected CNVRs, harboring genes related to neuronal development, reproduction, nutrition and energy metabolism. In addition, we found 85 CNVRs that are significantly different between domestic yak living in high- and low-altitude areas, including three genes related to hypoxia response and six related to immune defense. This analysis shows that genic CNVs may play an important role in phenotypic changes during yak domestication and adaptation to life at high-altitude. We present the first refined CNV map for yak along with comprehensive genomic analysis of yak CNV. Our results provide new insights into the genetic basis of yak domestication and adaptation to living in a high-altitude environment, as well as a valuable genetic resource that will facilitate future CNV association studies of important traits in yak and other bovid species.

[Genetic diversity of DNA microsatellite for Tibetan Yak].

PubMed

Li, Duo; Chai, Zhi-Xin; Ji, Qiu-Mei; Zhang, Cheng-Fu; Xin, Jin-Wei; Zhong, Jin-Cheng

2013-02-01

To assess the genetic diversity and relationship of the Tibetan yak breeds. The genetic diversity and phylogenies of a total of 480 individual from 11 Tibetan yak groups were analyzed using PCR and multiplex gel electrophoresis of silver staining technology with eight pairs of microsatellite markers.The result showed that these markers were highly polymorphic loci with rich genetic diversity in the Tibetan yak populations.The average polymorphic information content (PIC) in 11 groups of yak were higher than 0.5. The highest HEL13 was 0.8496, and the lowest TGLA57 was 0.7349. Among them, the PICof Dingqing yak was minimum (0.7505), indicating that the group is relatively pure.Sangri Yak had the highest PIC value (0.7949) indicating greater genetic variationwithinthe groups. Among the 11 groups examined, the order of heterozygosity size wasSangri(0.8193)>Jiangda(0.8190)>Sangsang(0.8157)>Baqing(0.8150)>Kangbu(0.8123)> Jiali(0.8087)>Gongbujiangda(0.8054)>Sibu(0.8041)>Leiwuqi(0.8033)>Pali(0.8031)>Dingqing(0.7831). The groups from eastern Tibet had grater genetic diversity than those from Western Tibet, which indicate that Tibet may be one of the cradles of the yak.According to the genetic distance, the cluster relationship constructed with UPGMA and NJ methods showed that 11 yak groups in Tibet could be divided into three forms. In summary,Tibet yak has abundant genetic diversity and the selected microsatellite markers can be used to evaluategenetic diversity of Tibetan yak.

Comparison of longissimus dorsi Fatty Acids Profiles in Gansu Black Yak and Chinese Yellow Cattle Steers and Heifers.

PubMed

Zhang, S G; Liu, T; Brown, M A; Wu, J P

2015-01-01

Fatty acid (FA) composition of longissimus dorsi intramuscular fat in Black Yak and Chinese Yellow Cattle were evaluated in 44 Black Yak and 41 Chinese Yellow Cattle of both genders. Interactions of species with gender were observed for total saturated fatty acid (SFA), unsaturated fatty acid (UFA), monounsaturated fatty acid (MUFA), polyunsaturated fatty acid (PUFA), palmitic acid, stearic acid, oleic acid, linolenic acid, arachidonic acid, EPA, and DHA concentrations, as well as PUFA/SFA ratio in the longissimus dorsi (p<0.05). The SFA percentage was greater in yellow cattle than yak in both genders but the species difference in heifers was greater than in steers (p<0.05). Yak had greater UFA, MUFA and PUFA percentages than yellow cattle in both steers and heifers (p<0.05) but the difference between yak and yellow cattle heifers was greater than yak and yellow cattle steers. The percentages of inolenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid; and PUFA/SFA were greater in yak than yellow cattle in both steers and heifers (p<0.05). In addition, the ratio of n-6/n-3 PUFA in yak was lesser than in yellow cattle (p<0.05). These results indicated that FA composition generally differed between yak and yellow cattle but the differences were not the same in heifers as compared to steers. Results also suggested that species differences in FA composition tended to favor Black Yak over Chinese Yellow Cattle, indicating that the longissimus dorsi of Black Yak may have a higher nutritive value than that of Chinese Yellow Cattle and potential for development as a desirable natural product.

Comparison of longissimus dorsi Fatty Acids Profiles in Gansu Black Yak and Chinese Yellow Cattle Steers and Heifers

PubMed Central

Zhang, S. G.; Liu, T.; Brown, M. A.; Wu, J. P.

2015-01-01

Fatty acid (FA) composition of longissimus dorsi intramuscular fat in Black Yak and Chinese Yellow Cattle were evaluated in 44 Black Yak and 41 Chinese Yellow Cattle of both genders. Interactions of species with gender were observed for total saturated fatty acid (SFA), unsaturated fatty acid (UFA), monounsaturated fatty acid (MUFA), polyunsaturated fatty acid (PUFA), palmitic acid, stearic acid, oleic acid, linolenic acid, arachidonic acid, EPA, and DHA concentrations, as well as PUFA/SFA ratio in the longissimus dorsi (p<0.05). The SFA percentage was greater in yellow cattle than yak in both genders but the species difference in heifers was greater than in steers (p<0.05). Yak had greater UFA, MUFA and PUFA percentages than yellow cattle in both steers and heifers (p<0.05) but the difference between yak and yellow cattle heifers was greater than yak and yellow cattle steers. The percentages of inolenic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid; and PUFA/SFA were greater in yak than yellow cattle in both steers and heifers (p<0.05). In addition, the ratio of n-6/n-3 PUFA in yak was lesser than in yellow cattle (p<0.05). These results indicated that FA composition generally differed between yak and yellow cattle but the differences were not the same in heifers as compared to steers. Results also suggested that species differences in FA composition tended to favor Black Yak over Chinese Yellow Cattle, indicating that the longissimus dorsi of Black Yak may have a higher nutritive value than that of Chinese Yellow Cattle and potential for development as a desirable natural product. PMID:26761841

Rennet-induced coagulation properties of yak casein micelles: A comparison with cow casein micelles.

PubMed

Zhang, Yan; Li, Yuan; Wang, Pengjie; Tian, Yanbao; Liang, Qi; Ren, Fazheng

2017-12-01

It is essential for yak cheese processing to understand the rennet-induced coagulation properties of gel formation from casein micelles. We have previously discovered that yak milk requires a longer incubation time but forms stronger gels compared with cow milk. In this study, we are aiming to understand the rennet-induced coagulation properties of yak casein micelles comparing with cow casein micelles. Rheological analyses revealed that the gelling times of yak and cow casein micelles were 11.6±0.5 and 8.7±0.4min (P<0.05) respectively, but yak casein gel had a higher elastic modulus G' (6.5±0.2Pa) than cow casein gel (2.5±0.2Pa; P<0.05). This is consistent with the results obtained by micro-rheology. Confocal laser scanning microscopic images (CLSM) and cryo-scanning electron microscopic images (cryo-SEM) showed that yak casein gel was more homogeneous and had smaller pore size than cow casein gels. Yak casein micelles had higher calcium (26.00mM), phosphate (19.90mM) and β-casein (relative 32%) concentrations. In addition, yak casein micelles were larger (Z-average 218.6nm) than cow casein micelles, and contained lower κ-casein (relative 13%). By comparison with cow casein micelles, yak casein micelle composition corresponding to their micellar calcium phosphate and κ-casein content may greatly contribute to the longer coagulation time and denser gel structure. An initial slower caseinomacropeptide (CMP) release rate and the slower rate of aggregation between para-casein micelles contributed to a more homogeneous yak gel network. Higher colloidal calcium phosphate is crucial for yak casein micelle aggregation and gel firmness because sufficient colloidal calcium phosphates can firmly glue sub-micelles and links casein micelles. This study provides valuable information for yak cheese production. Copyright © 2017. Published by Elsevier Ltd.

Morphological adaptations of yak (Bos grunniens) tongue to the foraging environment of the Qinghai-Tibetan Plateau.

PubMed

Shao, B; Long, R; Ding, Y; Wang, J; Ding, L; Wang, H

2010-08-01

Using light and scanning electron microscopy, the morphological adaptations of the yak (Bos grunniens) tongue to its foraging environment in the Qinghai-Tibetan Plateau were studied. The tongue of the yak was compared with that of cattle (Bos taurus). Compared with cattle, yak tongues are on average 4 cm shorter (P < 0.001), and yak consume forages using the labia oris, rather than by extending the tongue into the harsh environment. The lingual prominence of yak is greater (P < 0.001) and more developed than in cattle. The conical papillae on the prominence surface of yak are slightly larger (diameter: P = 0.068 and height: P = 0.761) and more numerous (P < 0.001) than in cattle. The lenticular papillae on the prominence surface of yak are larger (diameter: P = 0.002 and height: P = 0.115) and more numerous (P = 0.007) than in cattle. Such characteristics may improve the digestibility of forage by the grinding of food between the tongue and the upper palate. Filiform, conical, lenticular, fungiform, and vallate papillae were observed on the dorsal surface of the tongues studied; no foliate papillae were observed. The papillae were covered by keratinized epithelium, which was thicker (P < 0.001) in the yak than in cattle. It is suggested that the development of characteristic filiform papillae, and more numerous lingual gland ducts and mucus-secreting pores in the lenticular, fungiform and vallate papillae, fungiform papillae, probably having mechanical functions, are all morphological adaptations by yak to diets with greater fiber and DM content as provided by the plants within the Qinghai-Tibetan Plateau environment. On average, yak has 26 vallate papillae and cattle have 28. In the vallate papillae of the yak, the taste buds are arranged in a monolayer within the epithelium, whereas they are multilayered (2 to 4) in those papillae in cattle. The number of taste buds in each vallate papillae was less (P < 0.001) in the yak than in cattle. Therefore, the gustatory function of the yak was weaker than in cattle. Yaks graze throughout the year on diverse natural grasslands and have evolved morphological characteristics enabling them to consume a wide variety of plant species, thereby better adapting them to the typically harsh characteristics of their pastures.

The aggregation behavior and interactions of yak milk protein under thermal treatment.

PubMed

Wang, T T; Guo, Z W; Liu, Z P; Feng, Q Y; Wang, X L; Tian, Q; Ren, F Z; Mao, X Y

2016-08-01

The aggregation behavior and interactions of yak milk protein were investigated after heat treatments. Skim yak milk was heated at temperatures in the range of 65 to 95°C for 10 min. The results showed that the whey proteins in yak milk were denatured after heat treatment, especially at temperatures higher than 85°C. Sodium dodecyl sulfate-PAGE analysis indicated that heat treatment induced milk protein denaturation accompanied with aggregation to a certain extent. When the heating temperature was 75 and 85°C, the aggregation behavior of yak milk proteins was almost completely due to the formation of disulfide bonds, whereas denatured α-lactalbumin and β-lactoglobulin interacted with κ-casein. When yak milk was heated at 85 and 95°C, other noncovalent interactions were found between proteins including hydrophobic interactions. The particle size distributions and microstructures demonstrated that the heat stability of yak milk proteins was significantly lowered by heat treatment. When yak milk was heated at 65 and 75°C, no obvious changes were found in the particle size distribution and microstructures in yak milk. When the temperature was 85 and 95°C, the particle size distribution shifted to larger size trend and aggregates were visible in the heated yak milk. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Assessment of cattle genetic introgression into domestic yak populations using mitochondrial and microsatellite DNA markers

PubMed Central

Qi, X B; Jianlin, H; Wang, G; Rege, J E O; Hanotte, O

2010-01-01

Hybridization between yak Poephagus grunniens and taurine Bos taurus or indicine B. indicus cattle has been widely practiced throughout the yak geographical range, and gene flow is expected to have occurred between these species. To assess the impact of cattle admixture on domestic yak, we examined 1076 domestic yak from 29 populations collected in China, Bhutan, Nepal, India, Pakistan, Kyrgyzstan, Mongolia and Russia using mitochondrial DNA and 17 autosomal microsatellite loci. A cattle diagnostic marker-based analysis reveals cattle-specific mtDNA and/or autosomal microsatellite allele introgression in 127 yak individuals from 22 populations. The mean level of cattle admixture across the populations, calculated using allelic information at 17 autosomal microsatellite loci, remains relatively low (mYcattle = 2.66 ± 0.53% and Qcattle = 0.69 ± 2.58%), although it varies a lot across populations as well as among individuals within population. Although the level of cattle admixture shows a clear geographical structure, with higher levels of admixture in the Qinghai-Tibetan Plateau and Mongolian and Russian regions, and lower levels in the Himalayan and Pamir Plateau region, our results indicate that the level of cattle admixture is not significantly correlated with the altitude across geographical regions as well as within geographical region. Although yak-cattle hybridization is primarily driven to produce F1 hybrids, our results show that the subsequent gene flow between yak and cattle took place and has affected contemporary genetic make-up of domestic yak. To protect yak genetic integrity, hybridization between yak and cattle should be tightly controlled. PMID:19917041

Assessment of cattle genetic introgression into domestic yak populations using mitochondrial and microsatellite DNA markers.

PubMed

Qi, X B; Jianlin, H; Wang, G; Rege, J E O; Hanotte, O

2010-06-01

Hybridization between yak Poephagus grunniens and taurine Bos taurus or indicine B. indicus cattle has been widely practiced throughout the yak geographical range, and gene flow is expected to have occurred between these species. To assess the impact of cattle admixture on domestic yak, we examined 1076 domestic yak from 29 populations collected in China, Bhutan, Nepal, India, Pakistan, Kyrgyzstan, Mongolia and Russia using mitochondrial DNA and 17 autosomal microsatellite loci. A cattle diagnostic marker-based analysis reveals cattle-specific mtDNA and/or autosomal microsatellite allele introgression in 127 yak individuals from 22 populations. The mean level of cattle admixture across the populations, calculated using allelic information at 17 autosomal microsatellite loci, remains relatively low (mY(cattle) = 2.66 +/- 0.53% and Q(cattle) = 0.69 +/- 2.58%), although it varies a lot across populations as well as among individuals within population. Although the level of cattle admixture shows a clear geographical structure, with higher levels of admixture in the Qinghai-Tibetan Plateau and Mongolian and Russian regions, and lower levels in the Himalayan and Pamir Plateau region, our results indicate that the level of cattle admixture is not significantly correlated with the altitude across geographical regions as well as within geographical region. Although yak-cattle hybridization is primarily driven to produce F(1) hybrids, our results show that the subsequent gene flow between yak and cattle took place and has affected contemporary genetic make-up of domestic yak. To protect yak genetic integrity, hybridization between yak and cattle should be tightly controlled.

The yak genome and adaptation to life at high altitude.

PubMed

Qiu, Qiang; Zhang, Guojie; Ma, Tao; Qian, Wubin; Wang, Junyi; Ye, Zhiqiang; Cao, Changchang; Hu, Quanjun; Kim, Jaebum; Larkin, Denis M; Auvil, Loretta; Capitanu, Boris; Ma, Jian; Lewin, Harris A; Qian, Xiaoju; Lang, Yongshan; Zhou, Ran; Wang, Lizhong; Wang, Kun; Xia, Jinquan; Liao, Shengguang; Pan, Shengkai; Lu, Xu; Hou, Haolong; Wang, Yan; Zang, Xuetao; Yin, Ye; Ma, Hui; Zhang, Jian; Wang, Zhaofeng; Zhang, Yingmei; Zhang, Dawei; Yonezawa, Takahiro; Hasegawa, Masami; Zhong, Yang; Liu, Wenbin; Zhang, Yan; Huang, Zhiyong; Zhang, Shengxiang; Long, Ruijun; Yang, Huanming; Wang, Jian; Lenstra, Johannes A; Cooper, David N; Wu, Yi; Wang, Jun; Shi, Peng; Wang, Jian; Liu, Jianquan

2012-07-01

Domestic yaks (Bos grunniens) provide meat and other necessities for Tibetans living at high altitude on the Qinghai-Tibetan Plateau and in adjacent regions. Comparison between yak and the closely related low-altitude cattle (Bos taurus) is informative in studying animal adaptation to high altitude. Here, we present the draft genome sequence of a female domestic yak generated using Illumina-based technology at 65-fold coverage. Genomic comparisons between yak and cattle identify an expansion in yak of gene families related to sensory perception and energy metabolism, as well as an enrichment of protein domains involved in sensing the extracellular environment and hypoxic stress. Positively selected and rapidly evolving genes in the yak lineage are also found to be significantly enriched in functional categories and pathways related to hypoxia and nutrition metabolism. These findings may have important implications for understanding adaptation to high altitude in other animal species and for hypoxia-related diseases in humans.

Yak whole-genome resequencing reveals domestication signatures and prehistoric population expansions.

PubMed

Qiu, Qiang; Wang, Lizhong; Wang, Kun; Yang, Yongzhi; Ma, Tao; Wang, Zefu; Zhang, Xiao; Ni, Zhengqiang; Hou, Fujiang; Long, Ruijun; Abbott, Richard; Lenstra, Johannes; Liu, Jianquan

2015-12-22

Yak domestication represents an important episode in the early human occupation of the high-altitude Qinghai-Tibet Plateau (QTP). The precise timing of domestication is debated and little is known about the underlying genetic changes that occurred during the process. Here we investigate genome variation of wild and domestic yaks. We detect signals of selection in 209 genes of domestic yaks, several of which relate to behaviour and tameness. We date yak domestication to 7,300 years before present (yr BP), most likely by nomadic people, and an estimated sixfold increase in yak population size by 3,600 yr BP. These dates coincide with two early human population expansions on the QTP during the early-Neolithic age and the late-Holocene, respectively. Our findings add to an understanding of yak domestication and its importance in the early human occupation of the QTP.

Yak whole-genome resequencing reveals domestication signatures and prehistoric population expansions

PubMed Central

Qiu, Qiang; Wang, Lizhong; Wang, Kun; Yang, Yongzhi; Ma, Tao; Wang, Zefu; Zhang, Xiao; Ni, Zhengqiang; Hou, Fujiang; Long, Ruijun; Abbott, Richard; Lenstra, Johannes; Liu, Jianquan

2015-01-01

Yak domestication represents an important episode in the early human occupation of the high-altitude Qinghai-Tibet Plateau (QTP). The precise timing of domestication is debated and little is known about the underlying genetic changes that occurred during the process. Here we investigate genome variation of wild and domestic yaks. We detect signals of selection in 209 genes of domestic yaks, several of which relate to behaviour and tameness. We date yak domestication to 7,300 years before present (yr BP), most likely by nomadic people, and an estimated sixfold increase in yak population size by 3,600 yr BP. These dates coincide with two early human population expansions on the QTP during the early-Neolithic age and the late-Holocene, respectively. Our findings add to an understanding of yak domestication and its importance in the early human occupation of the QTP. PMID:26691338

Assessment of Russian VSTOL technology evaluating the YAK-38 'FORGER' and YAK-141 'FREESTYLE'

NASA Technical Reports Server (NTRS)

Nalls, Art; Stortz, Michael

1993-01-01

The dissolution of the Former Soviet Union (FSU) created new relationships between the world superpowers. Overnight, the Commonwealth of Independent States (CIS), formed from the remnants of the FSU, began the difficult transformation to a free market society. Military hardware that had once been highly classified and the basis for our own defense planning was now openly marketed at airshows around the world. 'Test' flights were available for potential customers and cooperative partnerships were explored between former adversaries. This environment permitted a visit to the Yakovlev Design Bureau, (YAK) for a vertical/short takeoff and landing (VSTOL) technology assessment. Yakovlev is the FSU's sole Design Bureau with experience in VSTOL aircraft and has developed two flying examples, the YAK-38 'FORGER' and YAK-141 'FREESTYLE'. This article reviews the performance of the YAK-38 'FORGER' and the YAK-141 'FREESTYLE'.

The distributions of Chinese yak breeds in response to climate change over the past 50 years.

PubMed

Wu, Jianguo

2016-07-01

The effects of prior climate change on yak breed distributions are uncertain. Here, we measured changes in the distributions of 12 yak breeds over the past 50 years in China and examined whether the changes could be attributed to climate change. Long-term records of yak breed distribution, grey relational analysis, fuzzy sets classification techniques and attribution methods were used. Over the past 50 years, the distributions of several yak breeds have changed in multiple directions, mainly shifting northward or westward, and most of these changes are related to the thermal index. Driven by climate change over the past years, the suitable range and the distribution centers of certain yak breeds have changed with fluctuation and have mainly shifted northward, eastward or southward. The consistency of observed versus predicted changes in distribution boundaries or distribution centers is higher for certain yak breeds. Changes in the eastern distribution boundary of two yak breeds over the past 50 years can be attributed to climate change. © 2015 Japanese Society of Animal Science.

Cloning and Expression of Yak Active Chymosin in Pichia pastoris

PubMed Central

Luo, Fan; Jiang, Wei Hua; Yang, Yuan Xiao; Li, Jiang; Jiang, Ming Feng

2016-01-01

Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production. Yaks total RNA was extracted from the abomasum of an unweaned yak. The yak preprochymosin, prochymosin, and chymosin genes from total RNA were isolated using gene specific primers based on cattle chymosin gene sequence respectively and analyzed their expression pattern byreal time-polymerase chain reaction. The result showed that the chymosin gene expression level of the sucking yaks was 11.45 times higher than one of adult yaks and yak chymosin belongs to Bovidae family in phylogenetic analysis. To express each, the preprochymosin, prochymosin, and chymosin genes were ligated into the expression vector pPICZαA, respectively, and were expressed in Pichia pastoris X33. The results showed that all the recombinant clones of P. pastoris containing the preprochymosin, prochymosin or chymosin genes could produce the active form of recombinant chymosin into the culture supernatant. Heterologous expressed prochymosin (14.55 Soxhlet unit/mL) had the highest enzyme activity of the three expressed chymosin enzymes. Therefore, we suggest that the yak chymosin gene recombinant Pichia pastoris strain could provide an alternative source of rennet production. PMID:27004812

Cloning and Expression of Yak Active Chymosin in Pichia pastoris.

PubMed

Luo, Fan; Jiang, Wei Hua; Yang, Yuan Xiao; Li, Jiang; Jiang, Ming Feng

2016-09-01

Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production. Yaks total RNA was extracted from the abomasum of an unweaned yak. The yak preprochymosin, prochymosin, and chymosin genes from total RNA were isolated using gene specific primers based on cattle chymosin gene sequence respectively and analyzed their expression pattern byreal time-polymerase chain reaction. The result showed that the chymosin gene expression level of the sucking yaks was 11.45 times higher than one of adult yaks and yak chymosin belongs to Bovidae family in phylogenetic analysis. To express each, the preprochymosin, prochymosin, and chymosin genes were ligated into the expression vector pPICZαA, respectively, and were expressed in Pichia pastoris X33. The results showed that all the recombinant clones of P. pastoris containing the preprochymosin, prochymosin or chymosin genes could produce the active form of recombinant chymosin into the culture supernatant. Heterologous expressed prochymosin (14.55 Soxhlet unit/mL) had the highest enzyme activity of the three expressed chymosin enzymes. Therefore, we suggest that the yak chymosin gene recombinant Pichia pastoris strain could provide an alternative source of rennet production.

[Comparative analysis of ISSR markers polymorphism in populations of yak (Bos mutus) and in F1 hybrids between yak and cattle in the Sayan-Altai region].

PubMed

Stolpovsky, Yu A; Kol, N V; Evsyukov, A N; Nesteruk, L V; Dorzhu, Ch M; Tsendsuren, Ts; Sulimova, G E

2014-10-01

The genetic variability in seven yak populations from the Sayan-Altai region and in F1 hybrids between yak and cattle (khainags) was investigated with the help of a technique that involves the use of inter simple sequence repeat (ISSR) markers generated with PCR primers (AG)9C and (GA)9C. Samples for the analysis were collected in Mongolia, Tuva, and Altai from 2008 through 2012. The examined yak populations differed in in the presence/absence of ISSR fragments, as well as in their frequency. In total, 46 ISSR fragments were identified using two marker systems; the proportion of polymorphic loci constituted 76% and 90% for the AG-ISSR and GA-ISSR markers, respectively. For the total sample of yaks, total genetic diversity (Ht), within-population diversity (Hs), and interpopulation diversity (Gst) constituted 0.081, 0.044, and 0.459 for the AG-ISSR and 0.137, 0.057, and 0.582 for the GA-ISSR markers, respectively. Based on ISSR finger printing, species- and breed-specific DNA patterns were described for the three groups of animals (yaks, cattle, khainags). For the domestic yak, the species-specific profile was represented by eight ISSR fragments. Genetic relationships between the yak populations, cattle breeds, and khainags were examined with the help of four different approaches used in the analysis of population structure: estimation of phylogenetic similarity, multidimensional scaling, principal component analysis, and cluster analysis. Clear evidence on the differentiation of the populations examined at the interspecific, as well as at intraspecific, level were obtained. Similar (relative); as well as remote (isolated), yak populations were identified. Khainags occupy an intermediate position between yak and cattle. However, the data on the ISSR-PCR marker polymorphism (genome polymorphism, population structure).indicate that part of the analyzed khainag genome was more similar to the yak genome than to the cattle genome.

Characteristics of yak platelet derived growth factors-alpha gene and expression in brain tissues.

PubMed

Huang, Zhenhua; Pan, Yangyang; Liu, Penggang; Yu, Sijiu; Cui, Yan

2017-05-29

Platelet derived growth factors (PDGFs) are key components of autocrine and paracrine signaling, both of which play important roles in mammalian developmental processes. PDGF expression levels also relate to oxygen levels. The characteristics of yak PDGFs, which are indigenous to hypoxic environments, have not been clearly described until the current study. We amplified the open reading frame encoding yak (Bos grunniens) platelet derived growth factor-a (PDGFA) from a yak skin tissue cDNA library by reverse transcriptase polymerase chain reaction (PCR) using specific primers and Sanger dideoxy sequencing. Expression of PDGFA mRNA in different portions of yak brain tissue (cerebrum, cerebellum, hippocampus, and spinal cord) was detected by quantitative real-time PCR (qRT-PCR). PDGFA protein expression levels and its location in different portions of the yak brain were evaluated by western blot and immunohistochemistry. We obtained a yak PDGFA 755 bp cDNA gene fragment containing a 636 bp open reading frame, encoding 211 amino acids (GenBank: KU851801). Phylogenetic analysis shows yak PDGFA to be well conserved, having 98.1% DNA sequence identity to homologous Bubalus bubalus and Bos taurus PDGFA genes. However, eight nucleotides in the yak DNA sequence and four amino acids in the yak protein sequence differ from the other two species. PDGFA is widely expressed in yak brain tissue, and furthermore, PDGFA expression in the cerebrum and cerebellum are higher than in the hippocampus and spinal cord (p > 0.05). PDGFA was observed by immunohistochemistry in glial cells of the cerebrum, cerebellum, and hippocampus, as well as in pyramidal cells of the cerebrum, and Purkinje cell bodies of the hippocampus, but not in glial cells of the spinal cord. The PDGFA gene is well conserved in the animal kingdom; however, the yak PDGFA gene has unique characteristics and brain expression patterns specific to this high elevation species.

Comparison of nitrogen utilization and urea kinetics between yaks (Bos grunniens) and indigenous cattle (Bos taurus).

PubMed

Zhou, J W; Zhong, C L; Liu, H; Degen, A A; Titgemeyer, E C; Ding, L M; Shang, Z H; Guo, X S; Qiu, Q; Li, Z P; Yang, G; Long, R J

2017-10-01

Under traditional management on the Qinghai-Tibetan Plateau, yaks () graze only on natural pasture without supplements and are forced to cope with sparse forage of low N content, especially in winter. In contrast, indigenous Tibetan yellow cattle () require supplements during the cold season. We hypothesized that, in response to harsh conditions, yaks cope with low N intakes better than cattle. To test this hypothesis, a study of whole-body N retention and urea kinetics was conducted in 2 concurrent 4 × 4 Latin squares, with 1 square using yaks and 1 square using cattle. Four isocaloric forage-concentrate diets differing in N concentrations (10.3, 19.5, 28.5, and 37.6 g N/kg DM) were formulated, and by design, DMI were similar between species and across diets. Urea kinetics were determined with continuous intravenous infusion of NN urea for 104 h, and total urine and feces were concomitantly collected. Urea production, urea recycling to the gut, and ruminal microbial protein synthesis all linearly increased ( < 0.001) with increasing dietary N in both yaks and cattle. Urinary N excretion was less ( = 0.04) and N retention was greater ( = 0.01) in yaks than in cattle. Urea production was greater in yaks than in cattle at the 3 lowest N diets but greater in cattle than in yaks at the highest N diet (species × diet, < 0.02). Urea N recycled to the gut ( < 0.001), recycled urea N captured by ruminal bacteria ( < 0.001), and ruminal microbial protein production ( = 0.05) were greater in yaks than in cattle. No more than 12% of urea recycling was through saliva, with no difference between species ( = 0.61). Glomerular filtration rate was lower ( = 0.05) in yaks than in cattle. The higher urea recycling and greater capture of recycled urea by ruminal microbes in yaks than in cattle suggest that yaks use mechanisms to utilize dietary N more efficiently than cattle, which may partially explain the better survival of yaks than cattle when fed low-N diets.

Development of an assay for rapid identification of meat from yak and cattle using polymerase chain reaction technique.

PubMed

Yin, R H; Bai, W L; Wang, J M; Wu, C D; Dou, Q L; Yin, R L; He, J B; Luo, G B

2009-09-01

Yak meat is of good quality with fine texture, high protein and low fat content, and rich in amino acids compared with that of cattle, and it lacks anabolic steroids or other drugs. In general terms, however, the meat yield of yak is relatively low compared with that of the cattle. In order to prevent possible adulteration of yak meat with cattle meat, based on the sequence of mitochondrial 12S rRNA gene, a multiplex PCR-based approach was proposed for rapid identification of the meat from yak and cattle using three primers designed in this work. Through the combinatorial usage of three primers with a single reaction set, two fragments of 290 and 159bp were amplified from the cattle meat DNA, whereas only a fragment of 290bp was obtained from the yak meat DNA. Using the assay described, satisfactory amplification was accomplished in the analysis of raw and heat-treated binary meat mixtures of yak/cattle with a detection limit of 0.1% for cattle meat. The technique is fast and straightforward. It might be a useful tool in the quality control of yak meat and meat products.

DNA-based authentication method for detection of yak (Bos grunniens) in meat products.

PubMed

Wang, Ping; Hu, Yue; Yang, Hairong; Han, Jiangxun; Zhao, Yongsheng; Chen, Ying

2013-01-01

A TaqMan probe real-time PCR method was developed for rapid detection of yak component in raw and cooked meat products. Specific primers and TaqMan probes of yak (Bos grunniens) were designed in the cytochrome b gene. The specificity of the method was evaluated using pure meat of eight yak breeds (Jiulong, Qinghai plateau, Maiwa, Gannan, Bazhou, Sibu, Zhongdian, and Jiali) samples and nine non-Bos grunniens animals (sheep, goat, pig, chicken, cattle, water buffalo, donkey, horse, and rabbit). DNA showed no cross-reaction with non-Bos grunniens animal DNA. This method proved to be sensitive in detecting the presence of low levels of target DNA obtained from 0.001% (w/w) component in a mixed meat sample. The method also successfully identified commercial yak meat products. The results showed that some yak meat might be involved in business fraud by using cattle meat (in this paper, cattle meat means meat of Bos taurus) instead of yak meat. In conclusion, real-time PCR assay used in this study was shown to be a rapid and sensitive method for detection of yak DNA in fresh meat and cooked meat products.

Brief Note :Variability in the cathelicidin 6 (CATHL-6) gene in Tianzhu white yak from Tibetan area in China.

PubMed

E, G X; Na, R S; Zhao, Y J; Chen, L P; Qiu, X Y; Huang, Y F

2015-04-10

Cathelicidins are a major family of antimicrobial peptides (AMPs), an important component of innate immune system, playing a critical role in host defense and disease resistance in virtually all living species. Polymorphism and functional studies on cathelicidin of Tianzhu white yak contribute to understanding the specific innate immune mechanism in animals living at high altitudes in comparison to cattle and domesticated white yak. Thirty-six individuals of Tianzhu white yak, originating from the area of three ecotypes (Gansu in China), were investigated. The total length of the aligned Yak cathelicidin 6 (CATHL-6) sequences was 1923 bp, including six single nucleotide polymorphisms and one indel. Ten haplotypes were identified, and phylogenetic analyses resolved those 10 haplotypes in two clusters. The results indicate that the white yak originated from two domestication sites. In addition, lack of significant pairwise difference between sequences (Tajima's D = 0.92865, P > 0.10) in the CATHL-6 region indicates absence of population size expansion in current white yak population.

Comparison of methanogen diversity of yak (Bos grunniens) and cattle (Bos taurus) from the Qinghai-Tibetan plateau, China.

PubMed

Huang, Xiao Dan; Tan, Hui Yin; Long, Ruijun; Liang, Juan Boo; Wright, André-Denis G

2012-10-19

Methane emissions by methanogen from livestock ruminants have significantly contributed to the agricultural greenhouse gas effect. It is worthwhile to compare methanogen from "energy-saving" animal (yak) and normal animal (cattle) in order to investigate the link between methanogen structure and low methane production. Diversity of methanogens from the yak and cattle rumen was investigated by analysis of 16S rRNA gene sequences from rumen digesta samples from four yaks (209 clones) and four cattle (205 clones) from the Qinghai-Tibetan Plateau area (QTP). Overall, a total of 414 clones (i.e. sequences) were examined and assigned to 95 operational taxonomic units (OTUs) using MOTHUR, based upon a 98% species-level identity criterion. Forty-six OTUs were unique to the yak clone library and 34 OTUs were unique to the cattle clone library, while 15 OTUs were found in both libraries. Of the 95 OTUs, 93 putative new species were identified. Sequences belonging to the Thermoplasmatales-affiliated Linage C (TALC) were found to dominate in both libraries, accounting for 80.9% and 62.9% of the sequences from the yak and cattle clone libraries, respectively. Sequences belonging to the Methanobacteriales represented the second largest clade in both libraries. However, Methanobrevibacter wolinii (QTPC 110) was only found in the cattle library. The number of clones from the order Methanomicrobiales was greater in cattle than in the yak clone library. Although the Shannon index value indicated similar diversity between the two libraries, the Libshuff analysis indicated that the methanogen community structure of the yak was significantly different than those from cattle. This study revealed for the first time the molecular diversity of methanogen community in yaks and cattle in Qinghai-Tibetan Plateau area in China. From the analysis, we conclude that yaks have a unique rumen microbial ecosystem that is significantly different from that of cattle, this may also help to explain why yak produce less methane than cattle.

Comparison of methanogen diversity of yak (Bos grunniens) and cattle (Bos taurus) from the Qinghai-Tibetan plateau, China

PubMed Central

2012-01-01

Background Methane emissions by methanogen from livestock ruminants have significantly contributed to the agricultural greenhouse gas effect. It is worthwhile to compare methanogen from “energy-saving” animal (yak) and normal animal (cattle) in order to investigate the link between methanogen structure and low methane production. Results Diversity of methanogens from the yak and cattle rumen was investigated by analysis of 16S rRNA gene sequences from rumen digesta samples from four yaks (209 clones) and four cattle (205 clones) from the Qinghai-Tibetan Plateau area (QTP). Overall, a total of 414 clones (i.e. sequences) were examined and assigned to 95 operational taxonomic units (OTUs) using MOTHUR, based upon a 98% species-level identity criterion. Forty-six OTUs were unique to the yak clone library and 34 OTUs were unique to the cattle clone library, while 15 OTUs were found in both libraries. Of the 95 OTUs, 93 putative new species were identified. Sequences belonging to the Thermoplasmatales-affiliated Linage C (TALC) were found to dominate in both libraries, accounting for 80.9% and 62.9% of the sequences from the yak and cattle clone libraries, respectively. Sequences belonging to the Methanobacteriales represented the second largest clade in both libraries. However, Methanobrevibacter wolinii (QTPC 110) was only found in the cattle library. The number of clones from the order Methanomicrobiales was greater in cattle than in the yak clone library. Although the Shannon index value indicated similar diversity between the two libraries, the Libshuff analysis indicated that the methanogen community structure of the yak was significantly different than those from cattle. Conclusion This study revealed for the first time the molecular diversity of methanogen community in yaks and cattle in Qinghai-Tibetan Plateau area in China. From the analysis, we conclude that yaks have a unique rumen microbial ecosystem that is significantly different from that of cattle, this may also help to explain why yak produce less methane than cattle. PMID:23078429

Pure cultures and characterization of yak Sertoli cells.

PubMed

Zhang, Hua; Liu, Ben; Qiu, Yuan; Fan, Jiang feng; Yu, Si jiu

2013-12-01

The culture of primary Sertoli cells has become an important resource in the study of their function. However, their use is limited because of contamination of isolated cells with other testicular cells, mainly germ cells. The aim was to establish technique to obtain pure yak Sertoli cells as well as to study the growth kinetics and biological characteristics of Sertoli cells in vitro. Two-step enzyme digestion was used to separate and culture yak Sertoli cells. Cultured using starvation method and the hypotonic treatment were also invented to get pure yak Sertoli cells. Furthermore, the purification of Yak Sertoli cells were identified according to their characteristics, such as bipolar corpuscular around the nucleus and expression of Fasl, in addition to their morphology. The average viability of the Sertoli cells was 97% before freezing and 94.5% after thawing, indicating that cryopreservation in liquid nitrogen had little influence on the viability of Sertoli cells. The growth tendency of yak Sertoli cells was similar to an S-shaped growth curve. Purified yak Sertoli cells frequently exhibited bipolar corpuscula in nucleus after Feulgen staining, and did have a positive reaction of Fasl by the immunocytochemical identification. After recovery chromosomal analysis of Sertoli cells had a normal chromosomal number of 60, comprising 29 pairs of autosomes and one pair of sex chromosomes. Assays for bacteria, fungi and mycoplasmas were negative. In conclusion, yak Sertoli cells have been successfully purified and cultured in vitro, and maintain stable biological characteristics after thawing. Therefore, it will not only preserve the genetic resources of yaks at the cellular level, but also provide valuable materials for transgenic research and feeder layer and nuclear donor cells in yak somatic cell cloning technology. Copyright © 2013 Elsevier Ltd. All rights reserved.

Duplication polymorphisms in exon 4 of κ-casein gene in yak breeds/populations.

PubMed

Pingcuo, S; Gao, J; Jiang, Z R; Jin, S Y; Fu, C Y; Liu, X; Huang, L; Zheng, Y C

2015-08-28

The objective of this study was to compare 12 bp-duplication polymorphisms in exon 4 of the κ-casein gene among 3 breeds/populations of yak (Bos grunniens). Genomic DNA was extracted from yak blood or muscle samples (N = 211) and a partial sequence of exon 4 of κ-casein gene was amplified by polymerase chain reaction. A polyacrylamide gel electrophoresis assay of the products (169 bp) revealed 2 variants. These variants differed in a 12-bp duplication of the nucleotide sequence corresponding to amino acids 147-150 (Glu-Ala-Ser-Pro) or 148-151 (Ala-Ser-Pro-Glu). The genotype frequency and gene frequency of the 2 κ-casein variants differed among the 3 yak breeds/populations. The long form of the κ-casein gene was the predominant allele, and the Jiulong yak showed the highest frequency of the short form variant of the κ-casein gene. In addition, 2 nucleotide differences resulting in amino acid substitutions were also identified in yaks. These results are significant for designing a breeding strategy to improve the genetic makeup of yak herds.

Effect of electrical stimulation and hot boning on the eating quality of Gannan yak longissimus lumborum.

PubMed

Lang, Yumiao; Sha, Kun; Zhang, Rui; Xie, Peng; Luo, Xin; Sun, Baozhong; Li, Haipeng; Zhang, Li; Zhang, Songshan; Liu, Xuan

2016-02-01

The objective of this study was to evaluate the effects of electrical stimulation (ES) versus non-electrical stimulation (NES) and type of boning (hot versus cold) on the eating quality of Gannan yak longissimus lumborum. Eighteen Gannan yak bulls were randomly divided into two groups: ES and NES. Hot boning (HB) and cold boning (CB) were applied to the left and right side of the carcasses, respectively. All of the four treatments missed the "ideal" pH/temperature window. HB reduced the rate of pH decline, decreased meat tenderness and water holding capacity. ES increased the rate of pH decline and improved yak meat tenderness (P<0.05); however, ES explained only 1% of the variation in WBSF. HB and ES had no significant effects on cooking loss, L* or b* values of yak meat. Postmortem aging increased yak meat tenderness and improved meat color parameters. HB had negative effects on yak meat quality, while ES could not reverse these deleterious effects. Copyright © 2015 Elsevier Ltd. All rights reserved.

Differences in mitochondrial gene expression profiles, enzyme activities and myosin heavy chain types in yak versus bovine skeletal muscles.

PubMed

Lin, Y Q; Xu, Y O; Yue, Y; Jin, S Y; Qu, Y; Dong, F; Li, Y P; Zheng, Y C

2012-08-29

Hypoxia can affect energy metabolism. We examined gene expression and enzyme activity related to mitochondrial energy metabolism, as well as myosin heavy chain (MyHC) types in yaks (Bos grunniens) living at high altitudes. Real-time quantitative PCR assays indicated that the yak has significantly lower levels of carnitine palmitoyltransferase (CPT) mRNA in the biceps femoris and lower levels of uncoupling protein 3 (UCP3) mRNA in both biceps femoris and longissimus dorsi than in Yellow cattle. No significant differences between yak and Yellow cattle were observed in the activities of mitochondrial β-hydroxyacyl-CoA dehydrogenase, isocitrate dehydrogenase and cytochrome oxidase in the same muscles. Semi-quantitative RT-PCR analysis showed that the MyHC 1 mRNA levels in yak biceps femoris was lower than in Yellow cattle. We conclude that the yak has significantly lower mRNA levels of CPT, UCP3, and MyHC 1 in biceps femoris than in Yellow cattle, suggesting that the yak biceps femoris has lower fatty acid oxidation capacity and greater glycolytic metabolic potential.

Influence of succinylation on physicochemical property of yak casein micelles.

PubMed

Yang, Min; Yang, Jitao; Zhang, Yuan; Zhang, Weibing

2016-01-01

Succinylation is a chemical-modification method that affects the physicochemical characteristics and functional properties of proteins. This study assessed the influence of succinylation on the physicochemical properties of yak casein micelles. The results revealed that surface hydrophobicity indices decreased with succinylation. Additionally, denaturation temperature and denaturation enthalpy decreased with increasing succinylation level, except at 82%. The buffering properties of yak casein micelles were affected by succinylation. It was found that chemical modification contributed to a slight shift of the buffering peak towards a lower pH value and a markedly increase of the maximum buffering values of yak casein micelles at pH 4.5-6.0 and pH < 3. Succinylation increased yak casein micellar hydration and whiteness values. The findings obtained from this study will provide the basic information on the physicochemical properties of native and succinylated yak casein micelles. Copyright © 2015 Elsevier Ltd. All rights reserved.

Characterization of the complete mitochondrial genome sequence of wild yak (Bos mutus).

PubMed

Chunnian, Liang; Wu, Xiaoyun; Ding, Xuezhi; Wang, Hongbo; Guo, Xian; Chu, Min; Bao, Pengjia; Yan, Ping

2016-11-01

Wild yak is a special breed in China and it is regarded as an important genetic resource for sustainably developing the animal husbandry in Tibetan area and enriching region's biodiversity. The complete mitochondrial genome of wild yak (16,322 bp in length) displayed 37 typical animal mitochondrial genes and A + T-rich (61.01%), with an overall G + C content of only 38.99%. It contained a non-coding control region (D-loop), 13 protein-coding genes, two rRNA genes, and 22 tRNA genes. Most of the genes have ATG initiation codons, whereas ND2, ND3, and ND5 genes start with ATA and were encoded on H-strand. The gene order of wild yak mitogenome is identical to that observed in most other vertebrates. The complete mitochondrial genome sequence of wild yak reported here could provide valuable information for developing genetic markers and phylogenetic analysis in yak.

Fluid-filled porosity of magmatic underplates from joint inversion of P and S receiver functions

NASA Astrophysics Data System (ADS)

Vinnik, Lev; Oreshin, Sergey; Makeyeva, Larissa; Dündar, Süleyman

2017-05-01

Vp/Vs ratio where Vp and Vs are P- and S-wave velocities is an indicator of rock composition, but estimates of Vp/Vs for the lower continental crust remain sparse. We present estimates of Vs, Vp and Vp/Vs in the crust of the Archean-Paleoproterozoic Siberian craton that are obtained by simultaneous inversion of P and S receiver functions from GSN seismograph stations NRIL, YAK and TIXI. These stations are located in the region of the Siberian traps (NRIL), close to the Laptev Sea Rift (TIXI) and the Viluy rift system (YAK). The most conspicuous result of our analysis is a high Vp/Vs ratio (≥2.0) at depths from 20-30 to 40 km. A very high Vp in this layer (from 7 to 8 km s-1) is indicative of magmatic underplating. We find broadly similar data in the western Mediterranean and in India. In a dry lower crust the Vp/Vs ratio is ∼1.8, which is hard to reconcile with the estimates >2.0. A coincidence in depths of zones of high electric conductivity and of anomalously high Vp/Vs in Siberia suggests that both may have the same origin: fluid-filled porosity. The porosity which is required by our seismic observations is on the order of 1 per cent. Origins of the fluids may be linked with processes of magmatic underplating.

Fluid-filled porosity of magmatic underplates from joint inversion of P and S receiver functions

NASA Astrophysics Data System (ADS)

Vinnik, Lev; Oreshin, Sergey; Makeyeva, Larissa; Dündar, Süleyman

2017-04-01

Vp/Vs ratio where Vp and Vs are P- and S-wave velocities is an indicator of rock composition, but estimates of Vp/Vs for the lower continental crust remain sparse. We present estimates of Vs, Vp and Vp/Vs in the crust of the Archean-Paleoproterozoic Siberian craton that are obtained by simultaneous inversion of P and S receiver functions from GSN seismograph stations NRIL, YAK and TIXI. These stations are located in the region of the Siberian traps (NRIL), close to the Laptev Sea Rift (TIXI) and the Viluy rift system (YAK). The most conspicuous result of our analysis is a high Vp/Vs ratio (≥2.0) at depths from 20-30 km to 40 km. A very high Vp in this layer (from 7 to 8 km/s) is indicative of magmatic underplating. We find broadly similar data in the western Mediterranean and in India. In a dry lower crust the Vp/Vs ratio is 1.8, which is hard to reconcile with the estimates > 2.0. A coincidence in depths of zones of high electric conductivity and of anomalously high Vp/Vs in Siberia suggests that both may have the same origin: fluid-filled porosity. The porosity which is required by our seismic observations is on the order of 1%. Origins of the fluids may be linked with processes of magmatic underplating.

PPARα signal pathway gene expression is associated with fatty acid content in yak and cattle longissimus dorsi muscle.

PubMed

Qin, W; Liang, C N; Guo, X; Chu, M; Pei, J; Bao, P J; Wu, X Y; Li, T K; Yan, P

2015-11-19

Intramuscular fatty acid (FA) is related to meat qualities such as juiciness, tenderness, palatability, and shear force. PPARα plays an important role in lipid metabolism in the liver and skeletal muscle. This study investigated FA composition in yaks and cattle, in order to ascertain whether a correlation between PPARα signal pathway genes as candidate genes and meat FA composition in yaks and cattle exists. Statistical analyses revealed that levels of monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) in yaks were significantly higher than those in cattle (P < 0.01), whereas saturated fatty acid (SFA) levels were significantly lower than those in cattle (P < 0.05). The mRNA expression levels of FABP4 (P < 0.05), SCP2 (P < 0.05), and APOA1 (P < 0.01) in yaks were significantly lower than those in cattle. However, LPL expression in yaks was significantly higher than that in cattle (P < 0.05). In yaks, the expression levels of FABP3 (P < 0.05) and LPL (P < 0.01) were negatively correlated with MUFA, and those of FABP4 and SCD were positively correlated with PUFA (P < 0.01). In cattle, the mRNA level of PLTP was positively correlated with SFA (P < 0.05), and LPL was positively correlated with MUFA (P < 0.05). These results suggest that these genes may participate in the regulation and control of intramuscular FA metabolism in yaks, so they could be used as candidate markers to improve yak meat quality.

Developmental competence of embryos derived from reciprocal in vitro fertilization between Yak (Bos grunniens) and cattle (Bos taurus).

PubMed

Zi, Xiang-Dong; Yin, Rong-Hua; Chen, Shao-Wei; Liang, Guan-Nan; Zhang, Da-Wei; Guo, Chun-Hua

2009-10-01

The purpose of this study was to investigate fertilization ability and embryo development to the blastocyst stage after reciprocal in vitro fertilization (IVF) between yak and cattle in an attempt to clarify the problem of low conception rate after mating yak females with cattle bulls. In vitro-matured (IVM) cattle and yak oocytes were inseminated with either Holstein or yak spermatozoa, and after an 18-h of coincubation period, a proportion of the oocytes was fixed and examined for sperm penetration, polyspermy and male pronuclear formation. The remaining oocytes were cultured in vitro and evaluated for cleavage and blastocyst formation rates. The percentage of IVM oocytes penetrated by spermatozoa ranged from 78.5 to 90.5%, and the formation of one or two pronuclei and the incidence of polyspermy did not differ among the different combinations. The cleavage and blastocyst rates were not affected by the species of the sperm, but they were affected by the species of the oocytes (P<0.05), with cattle oocytes having a higher (P<0.05) cleavage and blastocyst rates (69.9 and 31.3%) than yak oocytes (62.7 and 11.5%). The blastocyst formation rate was calculated from the cleaved zygotes. The interaction between sire and oocytes species (P<0.05) influenced blastocyst formation rate, with the highest blastocyst rate occurring in cattle oocytes fertilized with yak spermatozoa (36.5%) and the lowest rate occurring in yak oocytes fertilized with yak spermatozoa (9.4%). The effect of heterosis was apparent at the blastocyst stage, but there was a large reciprocal difference in blastocyst production between crosses. It was concluded that the low conception rate that results from crossing yaks with cattle is not due to either a species-specific block of fertilization or the developmental competence of the early stage embryo.

All particle energy spectrum of cosmic rays in 10 to the 15th power - 10 to the 20th power eV region

NASA Technical Reports Server (NTRS)

Krasilnikov, D. D.; Dyakonov, M. N.; Ivanov, A. A.; Kolosov, V. A.; Lischenyuk, F. F.; Sleptsov, I. Y.

1985-01-01

Average estimations of the shower energy components are presented and their sum gives E sub 0 (Rho sub 600) - an average function of the relation of E sub 0 with the shower size parameter Rho sub 600 measured at the Yakutsk extensive air showers (EAS) array. Using this relation to the EAS spectrum obtained at the Akeno and Yakutsk arrays the energy spectrum of the cosmic ray total flux within 15 lg (E sub 0,eV) 20 by the EAS methods is recovered.

Wildfires, smoke, and burn scars, near Yakutsk, Russia

NASA Technical Reports Server (NTRS)

2002-01-01

The Lena River in central Siberia is hidden beneath a veil of smoke from multiple wildfires burning around the city of Yakutsk, Russia. Fires have been burning in the region off and on since late May 2002, and may be agricultural in cause. This image was acquired by the Moderate Resolution Imaging Spectroradiometer (MODIS) on the Terra satellite on July 23, 2002. In the false=-color image, vegetation is bright green, smoke is blueish-white, and burned areas are reddish-brown. In both images, fire detections are marked with red outlines. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

Seroprevalence and risk factors of Chlamydia abortus infection in free-ranging white yaks in China.

PubMed

Qin, Si-Yuan; Huang, Si-Yang; Yin, Ming-Yang; Tan, Qi-Dong; Liu, Guang-Xue; Zhou, Dong-Hui; Zhu, Xing-Quan; Zhou, Ji-Zhang; Qian, Ai-Dong

2015-01-20

Chlamydia is gram-negative obligate bacteria which causes a wide variety of diseases in humans and animals. To date, there are a few reports about the seroprevalence of Chlamydia and the risk factors associated with Chlamydia infection in yaks in the world. In this study, 974 blood samples were collected from white yaks (Bos grunniens) in Tianzhu Tibetan Autonomous County, Gansu province, northwest China from June 2013 to April 2014. Antibodies against Chlamydia abortus were examined by the indirect hemagglutination (IHA) test, and 158 of 974 (16.22%) white yaks were seropositive for C. abortus antibodies at the cut-off of 1:16. The risk factors associated with seroprevalence were evaluated by a multivariate logistic regression analysis. Region, gender and age of white yak were left out of the final model, due to its insignificance in the logistic regression analysis (P > 0.05). However, season was considered as a major risk factor associated with C. abortus infection in white yaks. To our knowledge, this is the first survey of C. abortus seroprevalence in white yaks in China, which extends the host range for C. abortus and has important implications for public health and the local Tibetan economy.

Differential DNA methylation of the meiosis-specific gene FKBP6 in testes of yak and cattle-yak hybrids.

PubMed

Li, B; Luo, H; Weng, Q; Wang, S; Pan, Z; Xie, Z; Wu, W; Liu, H; Li, Q

2016-12-01

FK506-binding protein 6 (FKBP6) is essential for meiosis during mammalian spermatogenesis. However, the molecular regulation of FKBP6 during spermatogenesis remains unclear. In the present study, we performed molecular characterization of the meiosis-specific gene FKBP6 in yak testes. Yak FKBP6 encodes a polypeptide of 295 amino acid residues with an FK506-binding domain (FKBP_C) and three tetratricopeptide repeat domains. The methylation level of the FKBP6 promoter in testes was significantly higher in cattle-yak with male sterility than in yak, and the FKBP6 promoter was methylated in liver tissues in which FKBP6 is not expressed. FKBP6 promoter activity was significantly decreased after treatment with the M.SssI methyltransferase in vitro. Furthermore, the FKBP6 gene was remarkably activated in bovine mammary epithelial cells treated with the DNA methyltransferase inhibitor 5-aza-2-deoxycytidine. Taken together, our results demonstrate for the first time that the FKBP6 promoter is differentially methylated in testes; together with the functional promoter analysis, this suggests that methylation of this promoter may contribute to cattle-yak male infertility. © 2016 Blackwell Verlag GmbH.

Effects of Starvation on Lipid Metabolism and Gluconeogenesis in Yak

PubMed Central

Yu, Xiaoqiang; Peng, Quanhui; Luo, Xiaolin; An, Tianwu; Guan, Jiuqiang; Wang, Zhisheng

2016-01-01

This research was conducted to investigate the physiological consequences of undernourished yak. Twelve Maiwa yak (110.3±5.85 kg) were randomly divided into two groups (baseline and starvation group). The yak of baseline group were slaughtered at day 0, while the other group of yak were kept in shed without feed but allowed free access to water, salt and free movement for 9 days. Blood samples of the starvation group were collected on day 0, 1, 2, 3, 5, 7, 9 and the starved yak were slaughtered after the final blood sample collection. The liver and muscle glycogen of the starvation group decreased (p<0.01), and the lipid content also decreased while the content of moisture and ash increased (p<0.05) both in Longissimus dorsi and liver compared with the baseline group. The plasma insulin and glucose of the starved yak decreased at first and then kept stable but at a relatively lower level during the following days (p<0.01). On the contrary, the non-esterified fatty acids was increased (p<0.01). Beyond our expectation, the ketone bodies of β-hydroxybutyric acid and acetoacetic acid decreased with prolonged starvation (p<0.01). Furthermore, the mRNA expression of lipogenetic enzyme fatty acid synthase and lipoprotein lipase in subcutaneous adipose tissue of starved yak were down-regulated (p<0.01), whereas the mRNA expression of lipolytic enzyme carnitine palmitoyltransferase-1 and hormone sensitive lipase were up-regulated (p<0.01) after 9 days of starvation. The phosphoenolpyruvate carboxykinase and pyruvate carboxylase, responsible for hepatic gluconeogenesis were up-regulated (p<0.01). It was concluded that yak derive energy by gluconeogenesis promotion and fat storage mobilization during starvation but without ketone body accumulation in the plasma. PMID:26954191

A novel single nucleotide polymorphism in exon 7 of LPL gene and its association with carcass traits and visceral fat deposition in yak (Bos grunniens) steers.

PubMed

Ding, X Z; Liang, C N; Guo, X; Xing, C F; Bao, P J; Chu, M; Pei, J; Zhu, X S; Yan, P

2012-01-01

Lipoprotein lipase (LPL) is considered as a key enzyme in the lipid deposition and metabolism in tissues. It is assumed to be a major candidate gene for genetic markers in lipid deposition. Therefore, the polymorphisms of the LPL gene and associations with carcass traits and viscera fat content were examined in 398 individuals from five yak (Bos grunniens) breeds using PCR-SSCP analysis and DNA sequencing. A novel nucleotide polymorphism (SNP)-C→T (nt19913) was identified located in exon 7 in the coding region of the LPL gene, which replacement was responsible for a Phe-to-Ser substitution at amino acid. Two alleles (A and B) and three genotypes designed as AA, AB and BB were detected in the PCR products. The frequencies of allele A were 0.7928, 0.7421, 0.7357, 0.6900 and 0.7083 for Tianzhu white yak (WY), Gannan yak (GY), Qinghai-Plateau yak (PY), Xinjiang yak (XY) and Datong yak (DY), respectively. The SNP loci was in Hardy-Weinberg equilibrium in five yak populations (P>0.05). Polymorphism of LPL gene was shown to be associated with carcass traits and lipid deposition. Least squares analysis revealed that there was a significant effect on live-weight (LW) (P<0.01), average daily weight gain (ADG) and carcass weight (P<0.05). Individuals with genotype BB had lower mean values than those with genotype AA and AB for loin eye area and viscera fat weight (% of LW) in 25-36 months (P<0.05). The results indicated that LPL gene is a strong candidate gene that affects carcass traits and fat deposition in yak.

Effects of Starvation on Lipid Metabolism and Gluconeogenesis in Yak.

PubMed

Yu, Xiaoqiang; Peng, Quanhui; Luo, Xiaolin; An, Tianwu; Guan, Jiuqiang; Wang, Zhisheng

2016-11-01

This research was conducted to investigate the physiological consequences of undernourished yak. Twelve Maiwa yak (110.3±5.85 kg) were randomly divided into two groups (baseline and starvation group). The yak of baseline group were slaughtered at day 0, while the other group of yak were kept in shed without feed but allowed free access to water, salt and free movement for 9 days. Blood samples of the starvation group were collected on day 0, 1, 2, 3, 5, 7, 9 and the starved yak were slaughtered after the final blood sample collection. The liver and muscle glycogen of the starvation group decreased (p<0.01), and the lipid content also decreased while the content of moisture and ash increased (p<0.05) both in Longissimus dorsi and liver compared with the baseline group. The plasma insulin and glucose of the starved yak decreased at first and then kept stable but at a relatively lower level during the following days (p<0.01). On the contrary, the non-esterified fatty acids was increased (p<0.01). Beyond our expectation, the ketone bodies of β-hydroxybutyric acid and acetoacetic acid decreased with prolonged starvation (p<0.01). Furthermore, the mRNA expression of lipogenetic enzyme fatty acid synthase and lipoprotein lipase in subcutaneous adipose tissue of starved yak were down-regulated (p<0.01), whereas the mRNA expression of lipolytic enzyme carnitine palmitoyltransferase-1 and hormone sensitive lipase were up-regulated (p<0.01) after 9 days of starvation. The phosphoenolpyruvate carboxykinase and pyruvate carboxylase, responsible for hepatic gluconeogenesis were up-regulated (p<0.01). It was concluded that yak derive energy by gluconeogenesis promotion and fat storage mobilization during starvation but without ketone body accumulation in the plasma.

The complete mitochondrial genome sequence of the Datong yak (Bos grunniens).

PubMed

Wu, Xiaoyun; Chu, Min; Liang, Chunnian; Ding, Xuezhi; Guo, Xian; Bao, Pengjia; Yan, Ping

2016-01-01

Datong yak is a famous artificially cultivated breed in China. In the present work, we report the complete mitochondrial genome sequence of Datong yak for the first time. The total length of the mitogenome is 16,323 bp long, containing 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one non-coding region (D-loop region). The gene order of Datong yak mitogenome is identical to that observed in most other vertebrates. The overall base composition is 33.71% A, 25.8.0% C, 13.21% G and 27.27% T, with an A + T content of 60.98%. The complete mitogenome sequence information of Datong yak can provide useful data for further studies on molecular breeding and taxonomic status.

Characterization of the complete mitochondrial genome sequence of Gannan yak (Bos grunniens).

PubMed

Wu, Xiaoyun; Ding, Xuezhi; Chu, Min; Guo, Xian; Bao, Pengjia; Liang, Chunnian; Yan, Ping

2016-01-01

Gannan yak is the native breed of Gansu province in China. In this work, the complete mitochondrial genome sequence of Gannan yak was determined for the first time. The total length of the mitogenome is 16,322 bp long, with the base composition of 33.74% A, 25.84% T, 13.18% C, and 27.24% G. It contained 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one non-coding region (D-loop region). The gene order of Gannan yak mitogenome is identical to that observed in most other vertebrates. The complete mitogenome sequence information of Gannan yak can provide useful data for further studies on protection of genetic resources and phylogenetic relationships within Bos grunniens.

Establishment of mammary gland model in vitro: culture and evaluation of a yak mammary epithelial cell line.

PubMed

Fu, Mei; Chen, Yabing; Xiong, Xianrong; Lan, Daoliang; Li, Jian

2014-01-01

This study aimed to establish yak mammary epithelial cells (YMECs) for an in vitro model of yak mammary gland biology. The primary culture of YMECs was obtained from mammary gland tissues of lactating yak and then characterized using immunocytochemistry, RT-PCR, and western blot analysis. Whether foreign genes could be transfected into the YMECs were examined by transfecting the EGFP gene into the cells. Finally, the effect of Staphylococcus aureus infection on YMECs was determined. The established YMECs retained the mammary epithelial cell characteristics. A spontaneously immortalized yak mammary epithelial cell line was established and could be continuously subcultured for more than 60 passages without senescence. The EGFP gene was successfully transferred into the YMECs, and the transfected cells could be maintained for a long duration in the culture by continuous subculturing. The cells expressed more antimicrobial peptides upon S.aureus invasion. Therefore, the established cell line could be considered a model system to understand yak mammary gland biology.

Detection of cow milk adulteration in yak milk by ELISA.

PubMed

Ren, Q R; Zhang, H; Guo, H Y; Jiang, L; Tian, M; Ren, F Z

2014-10-01

In the current study, a simple, sensitive, and specific ELISA assay using a high-affinity anti-bovine β-casein monoclonal antibody was developed for the rapid detection of cow milk in adulterated yak milk. The developed ELISA was highly specific and could be applied to detect bovine β-casein (10-8,000 μg/mL) and cow milk (1:1,300 to 1:2 dilution) in yak milk. Cross-reactivity was <1% when tested against yak milk. The linear range of adulterant concentration was 1 to 80% (vol/vol) and the minimum detection limit was 1% (vol/vol) cow milk in yak milk. Different treatments, including heating, acidification, and rennet addition, did not interfere with the assay. Moreover, the results were highly reproducible (coefficient of variation <10%) and we detected no significant differences between known and estimated values. Therefore, this assay is appropriate for the routine analysis of yak milk adulterated with cow milk. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Prevalence and risk factors for brucellosis in domestic yak Bos grunniens and their herders in a transhumant pastoralist system of Dolpo, Nepal.

PubMed

Jackson, Daniel S; Nydam, Daryl V; Altier, Craig

2014-01-01

Disease caused by Brucella spp. represents the most common bacterial zoonotic infection worldwide. The distribution and public health impact of these infections in Nepal's mountain regions are poorly characterized. This cross sectional study assesses the burden of brucellosis on transhumant pastoralists and their yak in and around Shey Phoksundo National Park, Nepal. Objectives were to: (1) estimate individual animal prevalence of Brucella-seropositive yak, (2) identify herd- and individual-level risk factors associated with Brucella seropositivity in individual yak, and (3) identify herd-level risk factors associated with reported human brucellosis-like symptoms in a household. A case of household symptoms was defined as the reported occurrence within the previous year of at least one of three acute symptoms (chills, fever, night chills) and one of two chronic symptoms (joint pain, swollen joint(s)) in one or both of two individuals interviewed in a household. Two-hundred-ninety-seven yak from 61 herds were sampled, and 61 household questionnaires were completed. Estimated true prevalence was 0.22 (95% CI: 0.17; 0.28). Poisson regression with generalized estimating equations was used to account for repeated measures within a cluster (herd). Yak in herds reporting abortion occurrence within the previous year were 2.3 times more likely to be seropositive than those in herds not reporting abortion (95% CI: 1.2; 4.2, p = 0.01). For every 10 animal increase in herd number, individual animal seropositivity risk increased by 30% (95% CI: 10%; 50%, p = 0.001). Male yak were 0.7 times as likely to be seropositive as female yak (95% CI: 0.5; 0.9, p = 0.01). Three to five year old yak were 2 times more likely to be seropositive than yak <3 years old (95% CI: 1.3; 3.2, p = 0.003), and yak >5 years old were 4.9 times more likely to be seropositive than yak <3 years old (95% CI: 2.9; 8.1, p < 0.001). Risk of reported brucellosis-like symptoms at the household level was 2 (95% CI: 1.1; 3.5, p = 0.02) times greater for households with herds with >1 reactor, and was 3.6 (95% CI: 1.4; 9.2, p = 0.008) times greater for households reporting the practice of raw milk consumption. These results indicate that yak seropositivity for Brucella spp. is widespread in the region, and is associated with reported human disease. This epidemiologic understanding is essential to the identification of public health opportunities at the interface of Himalayan livestock populations and the transhumant pastoralists that depend on them.

Health and mineral nutrition status of yaks in southern Mustang, Nepal.

PubMed

Kumagai, Hajime; Nakajima, Mitsumi; Anzai, Hiroki; Sakai, Takashi; Oishi, Kazato; Hirooka, Hiroyuki; Shah, Manoj Kumar

2017-08-01

Biochemical values and mineral concentrations in blood plasma were investigated to evaluate the statuses of health and mineral nutrition among yaks in Mustang District, Nepal. In total, 118 plasma samples of female yaks collected in April and September/October of 2013-2015 were offered. Seventy-four percent of yaks showed lower plasma total-cholesterol concentrations than the lowest limit of reference range (100 mg/dL) and the values in spring (83.41 mg/dL) were lower (P < 0.05) than those in autumn (95.05 mg/dL). All the yaks had lower plasma albumin concentrations than the lowest limit of reference range (3.0 g/dL) and 66% of yaks showed lower plasma inorganic phosphorus concentrations than the critical level of phosphorus deficiency (4.5 mg/dL). Thirty-five percent of yaks showed lower plasma calcium concentrations than the lowest limit of normal range (8 mg/dL) and the concentrations were lower in spring than in autumn (P < 0.01). Seventy-five percent of yaks presented lower copper concentrations than the critical level (0.65 mg/L) and the concentrations were lower in spring than in autumn (P < 0.01). Since the low plasma total-cholesterol might have indicated shortage of dry matter and energy intake, attention should be paid to the nutritional statuses of energy, phosphorus, calcium and copper in winter and early spring. © 2016 Japanese Society of Animal Science.

Dietary intakes of energy and macronutrients by lactating women of different ethnic groups living in Yakutia

PubMed Central

Burtseva, Tatiana; Solodkova, Irina; Savvina, Maya; Dranaeva, Galina; Shadrin, Victor; Avrusin, Sergei; Sinelnikova, Elena; Chasnyk, Vyacheslav

2013-01-01

Background There should be a substantial increase in the intake of dietary energy, protein and other nutrients by lactating women, though these special increments can be different in different ethnic groups. Objective To evaluate the influence of maternal ethnicity and diet on the quality of breast milk and its potential effect on early childhood development. Design A total of 185 mothers (150 Native and 35 Russian) living in settlements and small towns of rural Yakutia and 54 mothers (26 Native and 28 Russian) living in Yakutsk were surveyed and average food intake was recorded during 3 successive days before the survey was analyzed. Results The amount of protein varied from 18 to 168.3 g/day, fat – from 12 to 176.1 g/day, energy – from 900 to 3680.4 kcal/day. Protein intake was at the level of current recommended dietary allowances (RDA) in Russians and was higher than in Natives living in rural settlements and small towns (p=0.02) and in Yakutsk (p=0.03). Carbohydrate intake was higher, though not significantly, in both ethnic groups compared with the current recommendations. Protein, fat, carbohydrates and, therefore, energy intake were lower (p<0.03) in Native women living in Yakutsk compared with the intake of Native women living in rural settlements and small towns. Conclusions The dietary intakes of energy and macronutrients depended on the place where a woman lived rather than on her ethnicity. Overall, energy intake was considered to be at the lower limit (basal energy expenditure 2002/2005) for lactating women, with the exception of Native women living in Yakutsk whose energy intake was below the lower limit. PMID:23971015

Dietary intakes of energy and macronutrients by lactating women of different ethnic groups living in Yakutia.

PubMed

Burtseva, Tatiana; Solodkova, Irina; Savvina, Maya; Dranaeva, Galina; Shadrin, Victor; Avrusin, Sergei; Sinelnikova, Elena; Chasnyk, Vyacheslav

2013-01-01

There should be a substantial increase in the intake of dietary energy, protein and other nutrients by lactating women, though these special increments can be different in different ethnic groups. To evaluate the influence of maternal ethnicity and diet on the quality of breast milk and its potential effect on early childhood development. A total of 185 mothers (150 Native and 35 Russian) living in settlements and small towns of rural Yakutia and 54 mothers (26 Native and 28 Russian) living in Yakutsk were surveyed and average food intake was recorded during 3 successive days before the survey was analyzed. The amount of protein varied from 18 to 168.3 g/day, fat--from 12 to 176.1 g/day, energy--from 900 to 3680.4 kcal/day. Protein intake was at the level of current recommended dietary allowances (RDA) in Russians and was higher than in Natives living in rural settlements and small towns (p = 0.02) and in Yakutsk (p = 0.03). Carbohydrate intake was higher, though not significantly, in both ethnic groups compared with the current recommendations. Protein, fat, carbohydrates and, therefore, energy intake were lower (p < 0.03) in Native women living in Yakutsk compared with the intake of Native women living in rural settlements and small towns. The dietary intakes of energy and macronutrients depended on the place where a woman lived rather than on her ethnicity. Overall, energy intake was considered to be at the lower limit (basal energy expenditure 2002/2005) for lactating women, with the exception of Native women living in Yakutsk whose energy intake was below the lower limit.

Lower Troposphere Stratification and Pollutant Transport over Siberia in April 2006

NASA Astrophysics Data System (ADS)

Paris, J.; Nedelec, P.; Ramonet, M.; Golitsyn, G. S.; Belan, B. D.; Granberg, I. G.; Arshinov, M. Y.; Athier, G.; Boumard, F.; Cousin, J.; Ciais, P.

2006-12-01

In-situ airborne measurement of trace gases CO2, CO and O3 were performed during an intensive campaign over Central and Eastern Siberia, as part of the YAK-AEROSIB project. The campaign took place in April 11-14, 2006. At that time, the region was a weak source of CO2 (<1 gC m-2 d-1) but a number of fires occurred (http://maps.geog.umd.edu) south of the flight track, over north-eastern China. A total of 26 vertical profiles were collected from the ground level up to 7 km along a flight track of 5000 km between Novossibirsk and Yakutsk. The signature of local and more remote pollution sources was observed, associated with layers of elevated CO2 and CO concentrations, typically higher than 390 ppm and 250 ppb respectively. In most layers, a positive correlation between CO2 and CO is observed. The spatial extent of these layers can be tracked coherently on profiles up to 800 km apart. Layers are encountered above 3500 m, but they are more marked above 5000 m. This agrees well with a previously established climatology of ozone and water vapour layers in the troposphere from the MOZAIC programme. The representation of such layers in current chemistry transport is challenging, due to the model's limited vertical resolution and vertical mixing parameterization. The CO vertical distribution indicated a high variability near the surface (140-300 ppbv), more stable, but still variable concentrations between 1 and 4 km (120-200 ppbv) and surprisingly elevated CO values aloft, reaching up to 250 ppbv in the Eastern part of the flight track. High ozone values were occasionally found in the free troposphere (50-60 ppbv) with one intrusion of stratospheric air and one occurrence of active photochemistry in the Kemerovo area. Elsewhere, the lack of correlation between CO and ozone suggests the presence of aged air masses, without active photochemistry. Systematic back-trajectories calculated for each profile pointed out to zonal flow conditions, bringing pollution from Europe to Siberia. Some air masses originating from China and Mongolia south of the flight track were however sampled further to the South-East. High altitude CO concentrations are higher in the southern part of the track. The complementary use of the MOPITT space-borne CO observations suggested that the high CO concentrations measured during the YAK-AEROSIB campaign were probably caused by the transport of polluted air from China.

Structural changes in slenderized yak hair induced by heat-humidity conditions using Raman spectroscopy

NASA Astrophysics Data System (ADS)

Liu, H. L.; Zhao, B. Y.; Yu, W. D.

2013-04-01

In this study, estimation of structure was accomplished with the use of deconvolution, secondary derivation and curve-fitting. The structural changes of slenderized yak hair treated by heat-humidity conditions were quantified by analyzing the disulfide bond (S-S), amide I and amide III regions. The results showed that the amount of the disulphide bond in the yak hair decreases with the increase of treating time. The secondary structure of yak hair transforms from the α-helix and β pleated to the disordered conformation during the heat humidity processing.

Physicochemical and functional properties of gelatin extracted from Yak skin.

PubMed

Xu, Mengqi; Wei, Lixin; Xiao, Yuancan; Bi, Hongtao; Yang, Hongxia; Du, Yuzhi

2017-02-01

Different molecular weight distribution (MWD) gelatin was extracted from Yak skin after enzymatic pretreatments and their physicochemical and functional properties (SDS-PAGE, UV-vis absorption spectra, DSC, FT-IR, Amino acid analysis, AFM, emulsibility and foamability) were analyzed. The gelatin was extracted by pepsin and got different MWD of Yak skin gelatin by controlling the enzymolysis time. The SDS-PAGE showed the MWD of the Yak skin gelatin. The UV-vis absorption turned out that the broad MWD of Yak skin gelatin had a higher maximum absorption peaks. The FT-IR and AFM indicated that the gelatin structures and microstructures changed with the change of the MWD. The broad MWD of the Yak skin gelatin had a higher denaturation temperature (T D ), and it was higher than most of the other mammals and marine biological gelatin. The broad MWD gelatin also had higher imino acids (proline and hydroxyproline) contents and lower foamability and emulsibility compared to the narrow MWD gelatin. These findings, obtained for the first time for Yak skin gelatin, showed that it has great potential for application as an alternative to commercial gelatin due to its good thermotolerance, particularly in the applications of the biological materials, stabilizer of thermo-tolerant and so on. Copyright © 2016 Elsevier B.V. All rights reserved.

Molecular characterization and phylogenetic analysis of a yak (Bos grunniens) κ-casein cDNA from lactating mammary gland.

PubMed

Bai, W L; Yin, R H; Dou, Q L; Jiang, W Q; Zhao, S J; Ma, Z J; Luo, G B; Zhao, Z H

2011-04-01

κ-Casein is one of the major proteins in the milk of mammals. It plays an important role in determining the size and specific function of milk micelles. We have previously identified and characterized a genetic variant of yak κ-casein by evaluating genomic DNA. Here, we isolate and characterize a yak κ-casein cDNA harboring the full-length open reading frame (ORF) from lactating mammary gland. Total RNA was extracted from mammary tissue of lactating female yak, and the κ-casein cDNA were synthesized by RT-PCR technique, then cloned and sequenced. The obtained cDNA of 660-bp contained an ORF sufficient to encode the entire amino acid sequence of κ-casein precursor protein consisting of 190 amino acids with a signal peptide of 21 amino acids. Yak κ-casein has a predicted molecular mass of 19,006.588 Da with a calculated isoelectric point of 7.245. Compared with the corresponding sequences in GenBank of cattle, buffalo, sheep, goat, Arabian camel, horse, and rabbit, yak κ-casein sequence had identity of 64.76-98.78% in cDNA, and identity of 44.79-98.42% and similarity of 53.65-98.42% in deduced amino acids, revealing a high homology with the other livestock species. Based on κ-casein cDNA sequences, the phylogenetic analysis indicated that yak κ-casein had a close relationship with that of cattle. This work might be useful in the genetic engineering researches for yak κ-casein.

Profiling of the yak skeletal muscle tissue gene expression and comparison with the domestic cattle by genome array.

PubMed

Wang, H B; Zan, L S; Zhang, Y Y

2014-01-01

Of all the mammals of the world, the yak lives at the highest altitude area of more than 3000 m. Comparison between yak and cattle of the low-altitude areas will be informative in studying animal adaptation to higher altitudes. To investigate the molecular mechanism involved in meat quality differences between the two Chinese special varieties Qinghai yak and Qinchuan cattle, 12 chemical-physical characteristics of the longissimus dorsi muscle related to meat quality were compared at the age of 36 months, and the gene expression profiles were constructed by utilizing the bovine genome array. Significant analysis of microarrays was used to identify the differentially expressed genes. Gene ontology and pathway analysis were performed by a free Web-based Molecular Annotation System 2.0. The results reveal ~11 000 probes representing about 10 000 genes that were detected in both the Qinghai yak and Qinchuan cattle. A total of 1922 genes were shown to be differentially expressed, 633 probes were upregulated and 1259 probes were downregulated in the muscle tissue of Qinghai yak that were mainly involved in ubiquitin-mediated proteolysis, muscle growth regulation, glucose metabolism, immune response and so on. Quantitative real-time PCR (qRT-PCR) was performed to validate some differentially expressed genes identified by microarray. Further analysis implied that animals living at a high altitude may supply energy by more active protein catabolism and glycolysis compared with those living in the plain areas. Our results establish the groundwork for further studies on yaks' meat quality and will be beneficial in improving the yaks' breeding by molecular biotechnology.

Multipolar anisotropy of E{sub 0} Greater-Than-Or-Slanted-Equal-To 10{sup 17} eV cosmic rays according to data of the Yakutsk array for studying extensive air showers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Glushkov, A. V., E-mail: a.v.glushkov@ikfia.ysn.ru

The results obtained by analyzing arrival directions for primary cosmic particles characterized by energies in the region E{sub 0} Greater-Than-Or-Slanted-Equal-To 10{sup 17} eV and zenith angles in the range {theta} Less-Than-Or-Slanted-Equal-To 60 Degree-Sign and detected at the Yakutsk array for studying extensive air showers (EASs) over the period spanning 1974 and 2009 are presented. It is shown that these events exhibit different anisotropies in different energy intervals.

Cloning and polymorphisms of yak lactate dehydrogenase B gene.

PubMed

Wang, Guosheng; Zhao, Xingbo; Zhong, Juming; Cao, Meng; He, Qinghua; Liu, Zhengxin; Lin, Yaqiu; Xu, Yaou; Zheng, Yucai

2013-06-05

The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak.

Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene

PubMed Central

Wang, Guosheng; Zhao, Xingbo; Zhong, Juming; Cao, Meng; He, Qinghua; Liu, Zhengxin; Lin, Yaqiu; Xu, Yaou; Zheng, Yucai

2013-01-01

The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak. PMID:23739677

Arabidopsis YAK1 regulates abscisic acid response and drought resistance.

PubMed

Kim, Dongjin; Ntui, Valentine Otang; Xiong, Liming

2016-07-01

Abscisic acid (ABA) is an important phytohormone that controls several plant processes such as seed germination, seedling growth, and abiotic stress response. Here, we report that AtYak1 plays an important role in ABA signaling and postgermination growth in Arabidopsis. AtYak1 knockout mutant plants were hyposensitive to ABA inhibition of seed germination, cotyledon greening, seedling growth, and stomatal movement. atyak1-1 mutant plants display reduced drought stress resistance, as evidenced by water loss rate and survival rate. Molecular genetic analysis revealed that AtYak1 deficiency led to elevated expression of stomatal-related gene, MYB60, and down-regulation of several stress-responsive genes. Altogether, these results indicate that AtYak1 plays a role as a positive regulator in ABA-mediated drought response in Arabidopsis. © 2016 Federation of European Biochemical Societies.

pH treatment as an effective tool to select the functional and structural properties of yak milk caseins.

PubMed

Liu, H N; Zhang, C; Zhang, H; Guo, H Y; Wang, P J; Zhu, Y B; Ren, F Z

2013-09-01

Qula is made from yak milk after defatting, acidifying, and drying. Yak milk caseins are purified from Qula by dissolving in alkali solution. The effects of different pH treatments on the functional and structural properties of yak milk caseins were investigated. Over a broad range of pH (from 6.0 to 12.0), functional properties of yak milk caseins, including solubility, emulsifying activities, and thermal characteristics, and the structural properties, including 1-anilino-8-naphthalene-sulfonate fluorescence, turbidity and particle diameter, were evaluated. The results showed that the yak milk casein yield increased as the pH increased from 6.0 to 12.0. The solubility dramatically increased as the pH increased from 6.0 to 8.0, and decreased as the pH increased from 9.0 to 12.0. The changes in emulsifying activity were not significant. Caseins were remarkably heat stable at pH 9.0. The turbidity of the casein solution decreased rapidly as the pH increased from 6.0 to 12.0, and the results suggested that reassembled casein micelles were more compact at low pH than high pH. At pH values higher than 8.0, the yield of yak milk caseins reached more than 80%. The highest solubility was at pH 8.0, the best emulsification was at pH 10.0 and the greatest thermal stability was at pH 9.0. According to the functional characteristics of yak milk caseins, alkali conditions (pH 8.0-10.0) should be selected for optimum production. These results suggested that pH-dependent treatment could be used to modify the properties of yak milk caseins by appropriate selection of the pH level. Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Organochlorine pesticides and polychlorinated biphenyls in grass, yak muscle, liver, and milk in Ruoergai high altitude prairie, the eastern edge of Qinghai-Tibet Plateau.

PubMed

Pan, Jing; Gai, Nan; Tang, Hua; Chen, Shu; Chen, Dazhou; Lu, Guohui; Yang, Yongliang

2014-09-01

In highland pastures, where no agricultural and industrial activities exist, organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) are believed to be mainly coming from water-soil-grass system which is subject to air-water and air-soil exchanges and atmospheric precipitation. Samples of grass and yak muscle, liver, and milk were measured for OCPs and PCBs in the summer and winter of 2011. The total concentrations of HCHs, DDTs, endosulfans, HCB, and PCBs in grass samples were in the range of 0.53-2.45, 1.6-6.0, 1.10-4.38, 0.30-1.24, 0.65-2.04 ng g(-1) dw (dry weight), with the means 1.38, 2.86, 2.06, 0.73, and 1.19 ng g(-1) dw, respectively. The mean concentrations of HCHs and DDTs in yak muscle were 1.65 and 0.55 ng g(-1) fw (fresh weight), respectively; no significant seasonal differences. The average total concentrations of HCHs, DDTs, HCB, endosulfans, and PCBs in yak milk were 4.46, 0.59, 1.00, 0.27, and 0.097 ng g(-1) fat, respectively. Among the POPs investigated, β-HCH and HCB were dominant in yak muscle and liver, whereas β-HCH dominated the yak milk. Consistent with the results of other studies, PCB 153, 138, and 180 were detected in yak milk that is in accordance with the case reported for farmed cow milk in China and other countries. A human health risk was conducted based on the intake of OCPs via consumptions of the yak muscle and milk. Since the daily intake of HCHs and DDTs was lower than WHO or USEPA's acceptable daily intake or minimal risk level, showing that the consumptions of the yak muscle and milk would not pose any immediate risk to local people. Copyright © 2014 Elsevier B.V. All rights reserved.

Molecular characterization and expression analysis of osteopontin cDNA from lactating mammary gland in yak (Bos grunniens).

PubMed

Bai, W L; Yang, R J; Yin, R H; Jiang, W Q; Luo, G B; Yin, R L; Zhao, S J; Li, C; Zhao, Z H

2012-04-01

Osteopontin (OPN) is a secreted phosphorylated glycoprotein. It has an important role in mammary gland development and lactation, as well as, is thought to be a potential candidate gene for lactation traits. In the present work, we isolated and characterized a full-length open reading frame (ORF) of yak OPN cDNA from lactating mammary tissue, and examined its expression pattern in mammary gland during different stages of lactation, as well as, the recombinant OPN protein of yak was expressed successfully in E. coli. The sequencing results indicated that the isolated cDNA was 1132-bp in length containing a complete ORF of 837-bp. It encoded a precursor protein of yak OPN consisting of 278 amino acid with a signal peptide of 16 amino acids. Yak OPN has a predicted molecular mass of 29285.975 Da and an isoelectric point of 4.245. It had an identity of 65.50-99.16% in cDNA, identity of 52.06-98.56% and similarity of 65.40-98.56% in deduced amino acids with the corresponding sequences of cattle, buffalo, sheep, goat, pig, human, and rabbit. The phylogenetic analysis indicated that yak OPN had the closest evolutionary relationship with that of cattle, and next buffalo. In mammary gland, yak OPN was generally transcribed in a declining pattern from colostrum period to dry period with an apparent increase of OPN expression being present in the late period of lactation compared with peak period of lactation. Western blot analysis indicated that His-tagged yak OPN protein expressed in E. coli could be recognized not only by an anti-His-tag antibody but also by an anti-human OPN antibody. These results from the present work provided a foundation for further insight into the role of OPN gene in yak lactation.

RNA-Seq analysis of yak ovary: improving yak gene structure information and mining reproduction-related genes.

PubMed

Lan, DaoLiang; Xiong, XianRong; Wei, YanLi; Xu, Tong; Zhong, JinCheng; Zhi, XiangDong; Wang, Yong; Li, Jian

2014-09-01

RNA-Seq, a high-throughput (HT) sequencing technique, has been used effectively in large-scale transcriptomic studies, and is particularly useful for improving gene structure information and mining of new genes. In this study, RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary. After Illumina-Solexa deep sequencing, 26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library. Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing. Gene structure refinement analysis showed that 7340 genes that were annotated in the yak genome could be extended at the 5' or 3' ends based on the alignments been the transcripts and the genome sequence. Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp, and 2267 of them were predicted to code proteins. BLAST analysis of the new transcripts showed that 1200?4933 mapped to the non-redundant (nr), nucleotide (nt) and/or SwissProt sequence databases. Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus (41.4%), Bos grunniens mutus (33.0%), Ovis aries (6.3%), Homo sapiens (2.8%), Mus musculus (1.6%) and other species. Functional analysis showed that these expressed genes were involved in various Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes pathways. GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction. The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance. Moreover, the results confirmed that RNA-Seq HT technology is highly advantageous in improving gene structure information and mining of new genes, as well as in providing valuable data to expand the yak genome information.

Yak response to high-altitude hypoxic stress by altering mRNA expression and DNA methylation of hypoxia-inducible factors.

PubMed

Xiong, Xianrong; Fu, Mei; Lan, Daoliang; Li, Jian; Zi, Xiangdong; Zhong, Jincheng

2015-01-01

Hypoxia-inducible factors (HIFs) are oxygen-dependent transcriptional activators, which play crucial roles in tumor angiogenesis and mammalian development, and regulate the transcription of genes involved in oxygen homeostasis in response to hypoxia. However, information on HIF-1α and HIF-2α in yak (Bos grunniens) is scarce. The complete coding region of yak HIF-2α was cloned, its mRNA expression in several tissues were determined, and the expression levels were compared with those of closely related low-altitude cattle (Bos taurus), and the methylation status of promoter regions were analyzed to better understand the roles of HIF-1α and HIF-2α in domesticated yak. The yak HIF-2α cDNA was cloned and sequenced in the present work reveals the evolutionary conservation through multiple sequence alignment, although 15 bases changed, resulting in 8 amino acid substitutions in the translated proteins in cattle. The tissue-specific expression results showed that HIF-1α is ubiquitously expressed, whereas HIF-2α expression is limited to endothelial tissues (kidney, heart, lung, spleen, and liver) and blood in yak. Both HIF-1α and HIF-2α expressions were higher in yak tissues than in cattle. The HIF-1α expression level is much higher in yak than cattle in these organs, except for the lung (P < 0.05), but the HIF-2α gene is significantly different in the heart, spleen, and kidney (P < 0.05). Furthermore, the methylation levels in the 5' flanking regulatory regions of HIF-1α and HIF-2α in yak kidney were significantly decreased than cattle counterparts (P < 0.05). Identifying these genes and the comparison of different expressions facilitates the understanding of the biological high-altitude hypoxic stress response mechanism and may assist current medical research to understand hypoxia-related diseases.

Cloning of cDNAs for H1F0, TOP1, CLTA and CDK1 and the effects of cryopreservation on the expression of their mRNA transcripts in yak (Bos grunniens) oocytes.

PubMed

Niu, Hui-Ran; Zi, Xiang-Dong; Xiao, Xiao; Xiong, Xian-Rong; Zhong, Jin-Cheng; Li, Jian; Wang, Li; Wang, Yong

2014-08-01

We cloned and sequenced four pivotal cDNAs involved in DNA structural maintenance (H1F0 and TOP1) and the cell cycle (CLTA and CDK1) from yak oocytes. In addition, we studied the consequences of freezing-thawing (F/T) processes on the expression of their mRNA transcripts in yak immature and in vitro matured (MII) oocytes. H1F0, TOP1, CLTA and CDK1 cDNAs were cloned from yak oocytes by reverse transcriptase-polymerase chain reaction (RT-PCR) strategy. The expression of their mRNA transcript analyses were performed upon fresh and frozen-thawed immature germinal vesicle (GV) and MII yak oocytes following normalization of transcripts with GAPDH by real-time PCR. The yak H1F0, TOP1, CLTA and CDK1 cDNA sequences were found to consist of CDK1 585, 2539, 740, and 894 bp, respectively. Their coding regions encoded 195, 768, 244, and 298 amino acids, respectively. The homology with that of cattle was very high (95.2%, 98.8%, 93.6%, and 89.5%, respectively nucleotide sequence level, and 94.3%, 98.2%, 87.7%, and 90.9%, respectively at the deduced amino acid level). The overall mRNA expression levels of these four transcripts were reduced by F/T process, albeit at different levels. TOP1 in GV-oocytes, and H1F0 and CDK1 in MII-oocytes of the yak were significantly down-regulated (P<0.05). This is the first isolation and characterization of H1F0, TOP1, CLTA, and CDK1 cDNAs from yak oocytes. The lower fertility and developmental ability of yak oocytes following fertilization after cryopreservation may be explained by the alterations to their gene expression profiles. Copyright © 2014 Elsevier Inc. All rights reserved.

Cold related mortalities and protection against cold in Yakutsk, eastern Siberia: observation and interview study

PubMed Central

Donaldson, G C; Ermakov, S P; Komarov, Y M; McDonald, C P; Keatinge, W R

1998-01-01

Objective To assess how effectively measures adopted in extreme cold in Yakutsk control winter mortality. Design Interviews to assess outdoor clothing and measure indoor temperatures; regressions of these and of delayed cause-specific mortalities on temperature. Setting Yakutsk, east Siberia, Russia. Subjects: All people aged 50-59 and 65-74 years living within 400 km of Yakutsk during 1989-95 and sample of 1002 men and women who agreed to be interviewed. Main outcome measures Daily mortality from all causes and from ischaemic heart, cerebrovascular, and respiratory disease. Results Mean temperature for October-March 1989-95 was −26.6°C. At 10.2°C people wore 3.30 (95% confidence interval 3.08 to 3.53) layers of clothing outdoors, increasing to 4.39 (4.13 to 4.66; P<0.0001) layers at −20°C. Thick coats, often of fur, replaced anoraks as temperature fell to −48.2°C. 82% of people went out each day when temperatures were 10.2°C to −20°C, but below −20°C the proportion fell steadily to 44% (35% to 53%) at −48.2°C (P<0.001), and overall shivering outdoors did not increase. Living room temperature was 17.9 (17.2 to 18.5)°C at 10.2°C outdoors, 19.6 (18.8 to 20.4)°C at −20°C, and 19.1 (18.6 to 19.6)°C at −48.2°C. Mortality from all causes and from ischaemic heart and respiratory disease was unaffected by the fall in temperature. Mortality from respiratory disease (daily deaths per million) rose from 4.7 (4.3 to 5.1) to 5.1 (4.4 to 5.7) (P=0.03), but this was offset by a fall in deaths from injury. Conclusions People in Yakutsk wore very warm clothing, and in extremely cold weather stayed indoors in warm housing, preventing the increases in mortality seen in winter in milder regions of the world. Only respiratory mortality rose, perhaps because of breathing cold air. Key messagesDeath rates from ischaemic heart, cerebrovascular, and respiratory disease and all causes have been shown to increase as air temperature fallsIn Yakutsk, Russia, mortality from cerebrovascular and ischaemic heart disease and all causes among people aged 50-59 and 65-74 was unchanged as temperature fell to −48.2°CMortality from respiratory disease increased as temperatures fell below −20°C but this was more than offset by a decrease in deaths from accidentsExceptionally warm clothing, with reduction of outdoor excursions at temperatures below −20°C, prevented overall outdoor cold stress PMID:9765165

PREVALENCE OF SULFONAMIDE AND FLORFENICOL RESISTANCE GENES IN ESCHERICHIA COLI ISOLATED FROM YAKS (BOS GRUNNIENS) AND HERDSMEN IN THE TIBETAN PASTURE.

PubMed

Zhang, Anyun; Yang, Yunfei; Wang, Hongning; Lei, Changwei; Xu, Changwen; Guan, Zhongbin; Liu, Bihui; Huang, Xi; Peng, Linyao

2015-07-01

To determine the antimicrobial susceptibility profiles and prevalence of resistance genes in Escherichia coli isolated from yaks (Bos grunniens) and herdsmen in nine plateau pastures in Tibet, we isolated 184 nonidentical strains of E. coli from yaks and herdsmen. Antimicrobial susceptibility testing of 15 antimicrobials was conducted and the prevalence of sulfonamide resistance genes (sul1, sul2, and sul3) and florfenicol resistance genes (floR, cfr, cmlA, fexA, pexA, and estDL136) was determined. Escherichia coli isolated from yaks had a high resistance rate to sulfamethoxazole (44%), sulphafurazole (40.4%), and florfenicol (11.4%). Escherichia coli isolated from herdsmen had a high resistance rate to sulfamethoxazole (57%) and sulphafurazole (51%). In addition, sul genes were present in 93% of sulfonamide-resistant isolates (84/90), and 17 floR genes and four cmlA genes were found in 19 florfenicol-resistant isolates. Even though florfenicol is prohibited from use in humans, three floR genes were detected in strains isolated from herdsmen. The three floR-positive isolates from herdsmen had pulsed-field gel electrophoresis patterns similar to isolates from yaks. In addition to documenting the sul and floR genes in E. coli isolated from yaks and herdsmen in the Tibetan pasture, we demonstrated the potential risk that antimicrobial-resistant E. coli could spread among herdsmen and yaks.

Socio-economic burden of parasitic infections in yaks from 1984 to 2017 on Qinghai Tibetan Plateau of China-A review.

PubMed

Li, Kun; Shahzad, Muhammad; Zhang, Hui; Jiang, Xiong; Mehmood, Khalid; Zhao, Xiaodong; Li, Jiakui

2018-07-01

Yak is an important animal for the Tibetans at Qinghai-Tibetan Plateau of China. The burden of parasitic diseases has been a major threat to the health of yaks at this region presenting a considerable socio-economic losses and impact to yak production and local nomads. Keeping in view, we collected the published papers from 1984 to 2017 on major parasitic infections in yaks by electronic literature search from five databases including CNKI, Google, PubMed, Science Direct and Web of Science. The prevalence of Eimeria, Babesia, Theileria, Hypodermosis, Cystic echinococcosis, Alveolar echinococcosis, Toxoplasma gondii, Neospora caninum, Cryptosporidium, Giardia duodenalis, Enterocytozoon bieneusi, Toxocara vitulorum, and Fascioliasis infection in yaks was found to be 48.02%, 13.06%, 36.11%, 59.85%, 16.93%, 0.99%, 20.50%, 5.14%, 10.00%, 3.68%, 4.07%, 22.23% and 28.7% respectively. Data presented are contemplated to enhance our current understanding on the major parasitic diseases of yaks at Qinghai Tibetan plateau, China. The main aim of this effort is to ameliorate the effects of the parasitic burden in this specie; so that, the attempts are made to minimize the incidence of these infections in future to raise the socio-economic levels of local community. Copyright © 2018 Elsevier B.V. All rights reserved.

Seasonal variations in the fatty acid profile of milk from yaks grazing on the Qinghai-Tibetan plateau.

PubMed

Ding, Luming; Wang, Yupeng; Kreuzer, Michael; Guo, Xusheng; Mi, Jiandui; Gou, Yujiao; Shang, Zhanhuan; Zhang, Ying; Zhou, Jianwei; Wang, Hucheng; Long, Ruijun

2013-11-01

An experiment was conducted to study the seasonal changes in the fatty acid profile of milk from yaks (Bos grunniens) when kept at altitudes of 3000 m above sea level (a.s.l.) and higher. Data and samples were collected in summer (July), autumn (September), winter (November) and spring (March) from ten lactating yaks (four in spring). The yaks grazed pastures adjacent to the farm building throughout the year. In spring only they received 0·6 kg crop by-products per day (dry matter basis). Fresh alpine grasses, available in summer and autumn, showed high concentrations of α-linolenic acid (46-51 g/100 g lipids) compared with the dry, yellow vegetation of winter and spring (16 g/100 g lipids). In autumn and summer, the milk fat had higher concentrations of polyunsaturated fatty acids than in winter. These polyunsaturated fatty acids were comprised of vaccenic acid, rumenic acid and α-linolenic acid, which are all considered beneficial to human health. The rare fatty acid, γ-linolenic acid, was also detected in yak milk, especially in the milk obtained in spring. The results suggest that yak milk, which is the most important basic food of the Tibetan herders, has the most favourable fatty acid profile when yaks grazed green pasture, which also corresponds to the period of highest milk production.

The Yak genome database: an integrative database for studying yak biology and high-altitude adaption

PubMed Central

2012-01-01

Background The yak (Bos grunniens) is a long-haired bovine that lives at high altitudes and is an important source of milk, meat, fiber and fuel. The recent sequencing, assembly and annotation of its genome are expected to further our understanding of the means by which it has adapted to life at high altitudes and its ecologically important traits. Description The Yak Genome Database (YGD) is an internet-based resource that provides access to genomic sequence data and predicted functional information concerning the genes and proteins of Bos grunniens. The curated data stored in the YGD includes genome sequences, predicted genes and associated annotations, non-coding RNA sequences, transposable elements, single nucleotide variants, and three-way whole-genome alignments between human, cattle and yak. YGD offers useful searching and data mining tools, including the ability to search for genes by name or using function keywords as well as GBrowse genome browsers and/or BLAST servers, which can be used to visualize genome regions and identify similar sequences. Sequence data from the YGD can also be downloaded to perform local searches. Conclusions A new yak genome database (YGD) has been developed to facilitate studies on high-altitude adaption and bovine genomics. The database will be continuously updated to incorporate new information such as transcriptome data and population resequencing data. The YGD can be accessed at http://me.lzu.edu.cn/yak. PMID:23134687

Sodium butyrate improves the cloned yak embryo viability and corrects gene expression patterns.

PubMed

Xiong, Xian-rong; Lan, Dao-liang; Li, Jian; Wang, Yong; Zhong, Jin-cheng

2015-02-01

Interspecies somatic cell nuclear transfer (iSCNT), a powerful tool in basic scientific research, has been used widely to increase and preserve the population of endangered species. Yak (Bos grunniens) is one of these species. Development to term of interspecies cloned yak embryos has not been achieved, possibly due to abnormal epigenetic reprogramming. Previous studies have demonstrated that treatment of intraspecies cloned embryos with (NaBu) significantly improves nuclear-cytoplasmic reprogramming and viability in vitro. Therefore, in this study, we evaluated the effect of optimal NaBu concentration and exposure time on preimplantation development of yak iSCNT embryos and on the expression patterns of developmentally important genes. The results showed that 8-cell rate, blastocyst formation rate and total cell number increased significantly compared with their untreated counterparts when yak iSCNT embryos were treated with 5 nM NaBu for 12 h after activation, but that the 2-cell stage embryo rate was not significantly different. The treatment of NaBu also increased significantly the expression levels of Oct-4 and decreased the expression levels of HDAC-2, Dnmt-1 and IGF-1; the expression patterns of these genes were more similar to that of their bovine-yak in vitro fertilization (BY-IVF) counterparts. The results described above indicated that NaBu treatment improved developmental competence in vitro and 'corrected' the gene expression patterns of yak iSCNT embryos.

The histological characteristics, age-related thickness change of skin, and expression of the HSPs in the skin during hair cycle in yak (Bos grunniens)

PubMed Central

Yang, Xue; Cui, Yan; Yue, Jing; He, Honghong; Yu, Chuan; Liu, Penggang; Liu, Jun; Ren, Xiandong; Meng, Yun

2017-01-01

Objective This experiment was conducted to study the histological characteristics, age-related thickness changes, and expression of HSPs in the skin of yak. Methods A total of 20 yaks (10 males and 10 females) were used. Different regions of the normal skin of three different ages (newborn, half-year-old and adult) of yaks were harvested for histological study and thickness measurement. Biopsy samples were taken from the scapula regions of the skin from the same five approximately 1-year-old yaks during the hair cycle (telogen, anagen and catagen). RT-PCR, western blot and immunohistochemistry methods using the mRNA and protein levels were used to detect the expression of HSP27, HSP70 and HSP90. RT-PCR method was used to detect the mRNA expression of CGI-58 and KDF1. The IPP6.0 software was used to analyze the immunohistochemistry and measure the thickness of the skin. Results The general histological structure of hairy yak skin was similar to other domestic mammals. The unique features included prominent cutaneous vascular plexuses, underdeveloped sweat glands, a large number of nasolabial glands in the nasolabial plate, and hair follicle groups composed of one primary follicle and several secondary follicles. The skin, epidermis and dermis thickness did vary significantly between different body regions and different ages. The thickness of the skin, epidermis and dermis increased from newborn to adult in yaks. Yak skin thickness decreased from dorsally to ventrally on the trunk. The skin on the lateral surface was thicker than the skin on the medial surface on the limbs. HSP27, HSP70 and HSP90 showed different expression patterns during the hair cycle using RT-PCR, western blot and immunohistochemistry methods. The expression of HSP27 mRNA and protein in the anagen stage was the highest, followed by the catagen stage, and the expression in the telogen stage was the lowest. The expression of HSP70 mRNA and protein in the telogen stage was the highest, followed by the anagen stage, and the expression in the catagen stage was the lowest. The expression of HSP90 mRNA and protein in the anagen stage was the highest, followed by the telogen stage, and the expression in the catagen stage was the lowest. HSPs were mainly expressed in the outer root sheath of hair follicle during the hair cycle, also expressed in epidermis, sebaceous gland and sweat gland in the skin of Yak. The expression of CGI-58 mRNA in the anagen stage was the highest, followed by the catagen stage, and the expression in the telogen stage was the lowest. The expression of KDF1 mRNA in the telogen stage was the highest, followed by the catagen stage, and the expression in the anagen stage was the lowest. Meaning In this study, we examined and fully described the histology of normal skin in Yak and measured the skin thickness of different ages and different regions in Yak. These data may be useful to better understand and appreciate the adaptability features of yak skin. Our investigation reports the expression patterns of HSPs in yak skin for the first time. The different expression pattern of HSPs during the hair cycle suggests they may play different roles in yak hair follicle biology. PMID:28463974

The histological characteristics, age-related thickness change of skin, and expression of the HSPs in the skin during hair cycle in yak (Bos grunniens).

PubMed

Yang, Xue; Cui, Yan; Yue, Jing; He, Honghong; Yu, Chuan; Liu, Penggang; Liu, Jun; Ren, Xiandong; Meng, Yun

2017-01-01

This experiment was conducted to study the histological characteristics, age-related thickness changes, and expression of HSPs in the skin of yak. A total of 20 yaks (10 males and 10 females) were used. Different regions of the normal skin of three different ages (newborn, half-year-old and adult) of yaks were harvested for histological study and thickness measurement. Biopsy samples were taken from the scapula regions of the skin from the same five approximately 1-year-old yaks during the hair cycle (telogen, anagen and catagen). RT-PCR, western blot and immunohistochemistry methods using the mRNA and protein levels were used to detect the expression of HSP27, HSP70 and HSP90. RT-PCR method was used to detect the mRNA expression of CGI-58 and KDF1. The IPP6.0 software was used to analyze the immunohistochemistry and measure the thickness of the skin. The general histological structure of hairy yak skin was similar to other domestic mammals. The unique features included prominent cutaneous vascular plexuses, underdeveloped sweat glands, a large number of nasolabial glands in the nasolabial plate, and hair follicle groups composed of one primary follicle and several secondary follicles. The skin, epidermis and dermis thickness did vary significantly between different body regions and different ages. The thickness of the skin, epidermis and dermis increased from newborn to adult in yaks. Yak skin thickness decreased from dorsally to ventrally on the trunk. The skin on the lateral surface was thicker than the skin on the medial surface on the limbs. HSP27, HSP70 and HSP90 showed different expression patterns during the hair cycle using RT-PCR, western blot and immunohistochemistry methods. The expression of HSP27 mRNA and protein in the anagen stage was the highest, followed by the catagen stage, and the expression in the telogen stage was the lowest. The expression of HSP70 mRNA and protein in the telogen stage was the highest, followed by the anagen stage, and the expression in the catagen stage was the lowest. The expression of HSP90 mRNA and protein in the anagen stage was the highest, followed by the telogen stage, and the expression in the catagen stage was the lowest. HSPs were mainly expressed in the outer root sheath of hair follicle during the hair cycle, also expressed in epidermis, sebaceous gland and sweat gland in the skin of Yak. The expression of CGI-58 mRNA in the anagen stage was the highest, followed by the catagen stage, and the expression in the telogen stage was the lowest. The expression of KDF1 mRNA in the telogen stage was the highest, followed by the catagen stage, and the expression in the anagen stage was the lowest. In this study, we examined and fully described the histology of normal skin in Yak and measured the skin thickness of different ages and different regions in Yak. These data may be useful to better understand and appreciate the adaptability features of yak skin. Our investigation reports the expression patterns of HSPs in yak skin for the first time. The different expression pattern of HSPs during the hair cycle suggests they may play different roles in yak hair follicle biology.

Effects of yak-activated protein on hematopoiesis and related cytokines in radiation-induced injury in mice.

PubMed

Duan, Yabin; Yao, Xingchen; Zhu, Junbo; Li, Yongping; Zhang, Juanling; Zhou, Xuejiao; Qiao, Yijie; Yang, Meng; Li, Xiangyang

2017-12-01

The aim of the present study was to investigate the protective effects of yak-activated protein on hematopoiesis and cytokine function in radiation-induced injury in mice. A total of 180 Kunming mice were randomly divided into three groups (A, B and C). Of these, 60 were randomly divided into a normal control group, a radiation model group, a positive control group and 3 yak-activated protein groups (high, medium and low dose groups; 10, 5 and 2.5 mg/kg, respectively). The other 120 mice were used for the subsequent experiments on days 7 and 14 following radiation. Yak-activated protein was administered orally to mice in the treatment groups and an equal volume of saline was administered orally to mice in the normal control and radiation model groups for 14 days. The positive control group received amifostine (150 mg/kg) via intraperitoneal injection. With the exception of the control group, the groups of mice received a 5 Gy quantity of X-radiation evenly over their whole body once. Changes in the peripheral hemogram, thymus and spleen indices, DNA content in the bone marrow, interleukin (IL)-2 and IL-6 levels, and the expression levels of B cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) following irradiation were assessed. The low dose of yak-activated protein significantly increased Spleen indices in mice 14 days after irradiation and the high and middle dose of yak-activated protein significantly increased Thymus indices in mice 14 days after irradiation (P<0.05) compared with the control group. In addition, hemogram results increased gradually in the low-yak-activated protein dose group and were significantly higher 7 days after irradiation compared with the radiation model group (P<0.05). The DNA content in the bone marrow was markedly increased in the yak-activated protein groups, and increased significantly in the low dose group at 7 days post-irradiation compared with the radiation model group (P<0.05). The IL-2 content was significantly increased in the yak-activated protein groups (P<0.05). Furthermore, Bcl-2 expression was increased and Bax expression was decreased (P<0.05). These results suggest that yak-activated protein exerts protective effects against radiation-induced injury in mice. The optimal effects of yak-activated protein were observed in the medium dose group 14 days after irradiation.

Effects of yak-activated protein on hematopoiesis and related cytokines in radiation-induced injury in mice

PubMed Central

Duan, Yabin; Yao, Xingchen; Zhu, Junbo; Li, Yongping; Zhang, Juanling; Zhou, Xuejiao; Qiao, Yijie; Yang, Meng; Li, Xiangyang

2017-01-01

The aim of the present study was to investigate the protective effects of yak-activated protein on hematopoiesis and cytokine function in radiation-induced injury in mice. A total of 180 Kunming mice were randomly divided into three groups (A, B and C). Of these, 60 were randomly divided into a normal control group, a radiation model group, a positive control group and 3 yak-activated protein groups (high, medium and low dose groups; 10, 5 and 2.5 mg/kg, respectively). The other 120 mice were used for the subsequent experiments on days 7 and 14 following radiation. Yak-activated protein was administered orally to mice in the treatment groups and an equal volume of saline was administered orally to mice in the normal control and radiation model groups for 14 days. The positive control group received amifostine (150 mg/kg) via intraperitoneal injection. With the exception of the control group, the groups of mice received a 5 Gy quantity of X-radiation evenly over their whole body once. Changes in the peripheral hemogram, thymus and spleen indices, DNA content in the bone marrow, interleukin (IL)-2 and IL-6 levels, and the expression levels of B cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) following irradiation were assessed. The low dose of yak-activated protein significantly increased Spleen indices in mice 14 days after irradiation and the high and middle dose of yak-activated protein significantly increased Thymus indices in mice 14 days after irradiation (P<0.05) compared with the control group. In addition, hemogram results increased gradually in the low-yak-activated protein dose group and were significantly higher 7 days after irradiation compared with the radiation model group (P<0.05). The DNA content in the bone marrow was markedly increased in the yak-activated protein groups, and increased significantly in the low dose group at 7 days post-irradiation compared with the radiation model group (P<0.05). The IL-2 content was significantly increased in the yak-activated protein groups (P<0.05). Furthermore, Bcl-2 expression was increased and Bax expression was decreased (P<0.05). These results suggest that yak-activated protein exerts protective effects against radiation-induced injury in mice. The optimal effects of yak-activated protein were observed in the medium dose group 14 days after irradiation. PMID:29285056

Crustal Structure of the Yakutat Microplate: New Parameters for Understanding the Evolution of the Chugach-St.Elias Orogeny

NASA Astrophysics Data System (ADS)

Worthington, L. L.; Christeson, G. L.; van Avendonk, H. J.; Gulick, S. P.

2009-12-01

We present results of a 2008 marine seismic reflection/refraction survey acquired as part of the St. Elias Erosion and Tectonics Project (STEEP), a multi-disciplinary NSF-Continental Dynamics project aimed at tectonic-climate interaction, structural evolution and geodynamics in the Chugach-St. Elias orogen. The Chugach-St.Elias orogen is the result of flat-slab subduction and collision of the Yakutat (YAK) microplate with North Amercian (NA) on the southern Alaska margin during the last ~10Ma. A fundamental goal of STEEP is to address controversy related to the deep crustal structure of the YAK block itself, describe its offshore structural relationships and constrain its buoyancy in order to understand the orogenic driver. Marine seismic reflection profiles acquired across the offshore YAK microplate provide the first regional images of the top of the subducting YAK basement. The basement reflector is observed near the seafloor at the Dangerous River Zone (DRZ) and is overlain by up to 12 km of sediments near Kayak Island, resulting in a basement dip of ~3° in the direction of subduction. The basement reflector also shallows near the shelf-edge adjacent to the Transition Fault, the YAK-Pacific boundary. These observations are indicative of an overall regional basement tilt towards the NA continent. Two coincident wide-angle refraction profiles constrain YAK crustal thickness between 30-35km, >20km thicker than normal oceanic crust, and lower crustal velocities potentially >7km/s. Crustal velocity and thickness are comparable to the Kerguelen oceanic plateau and the Siletz terrane. These results are the first direct observations in support of the oceanic plateau theory for the origin of the YAK microplate. Crustal velocity and structure are continuous across the DRZ on the YAK shelf, which is historically described as a vertical boundary between continental crust on the east and oceanic basement on the west. Instead, we observe a gradual shallowing of elevated crustal velocities associated with the aforementioned basement high near DRZ. Interestingly, observed Moho arrivals across the profile do not mimic the dipping trajectory of the basement reflector, indicating that the YAK slab may be slightly wedge-shaped, thinning in the direction of subduction. If true, the following implications for the YAK-NA collision must be considered: first, that uplift and deformation have intensified through time as thicker, more buoyant YAK crust attempts to subduct; second, migration of intense uplift from west to east across the orogen is partly controlled by underlying slab structure at depth.

Prevalence, Associated Risk Factors, and Phylogenetic Analysis of Toxocara vitulorum Infection in Yaks on the Qinghai Tibetan Plateau, China

PubMed Central

Li, Kun; Lan, Yanfang; Luo, Houqiang; Zhang, Hui; Liu, Dongyu; Zhang, Lihong; Gui, Rui; Wang, Lei; Shahzad, Muhammad; Sizhu, Suolang; Li, Jiakui; Chamba, Yangzom

2016-01-01

Toxocara vitulorum has been rarely reported in yaks at high altitudes and remote areas of Sichuan Province of Tibetan Plateau of China. The current study was designed to investigate the prevalence, associated risk factors, and phylogenetic characteristics of T. vitulorum in yak calves on the Qinghai Tibetan plateau. Fecal samples were collected from 891 yak calves and were examined for the presence of T. vitulorum eggs by the McMaster technique. A multivariable logistic regression model was employed to explore variables potentially associated with exposure to T. vitulorum infection. T. vitulorum specimens were collected from the feces of yaks in Hongyuan of Sichuan Province, China. DNA was extracted from ascaris. After PCR amplification, the sequencing of ND1 gene was carried out and phylogenetic analyses was performed by MEGA 6.0 software. The results showed that 64 (20.1%; 95% CI 15.8–24.9%), 75 (17.2; 13.8–21.1), 29 (40.9; 29.3–53.2), and 5 (7.6; 2.5–16.8) yak calves were detected out to excrete T. vitulorum eggs in yak calve feces in Qinghai, Tibet, Sichuan, and Gansu, respectively. The present study revealed that high infection and mortality by T. vitulorum is wildly spread on the Qinghai Tibetan plateau, China by fecal examination. Geographical origin, ages, and fecal consistencies are the risk factors associated with T. vitulorum prevalence by logistic regression analysis. Molecular detection and phylogenetic analysis of ND1 gene of T. vitulorum indicated that T. vitulorum in the yak calves on the Qinghai Tibetan plateau are homologous to preveiously studies reported. PMID:27853122

Molecular characterization and expression profile of the melatonin receptor MT1 in the ovary of Tianzhu white yak (Bos grunniens).

PubMed

Hu, Jun Jie; Zhang, Xiao Yu; Zhang, Yong; Zhao, Xing Xu; Li, Fa Di; Tao, Jin Zhong

2017-02-01

Melatonin plays crucial roles in a wide range of ovarian physiological functions via the melatonin receptors (MRs). Structure and function of MRs have been well studied in sheep, cattle, and humans, but little information exists on the genetic characterization and function of these receptors in the ovary of the white yak. In the present study, the melatonin receptor MT1 was cloned by RT-PCR in the ovary of white yak; the MT1 cDNA fragment obtained (843bp) comprised an open reading frame (827bp) encoding a protein containing 275 residues, characterized by seven transmembrane regions and an NRY motif, two distinct amino acid replacements were found. The white yak MT1 had a 83.9-98.6% protein sequence identity with that of nine other mammals. Using RT-PCR, the expression levels of MT1, MT2, and LHR in the ovary of pregnant and non-pregnant white yaks were compared, revealing higher levels of all genes in pregnant yaks: 3.83-fold increase for MT1 (P<0.05), 1.39-fold increase for MT2, and 15.32-fold increase for LHR (P<0.05). The distribution of MT1 in yak ovaries was observed using immunohistochemistry on paraffin embedded ovarian sections: MT1 was mainly present on primordial follicles (PF), granulosa cells (GCs), oocytes, and corpus luteum (CL) cells; MT1 expression showed an increasing tendency from PF to GCs to oocytes and to large CL cells. It is suggested that melatonin and MT1 are associated with the corpus luteum function of pregnancy maintenance and follicular development during oocyte maturation in the white yak. Copyright © 2015 Elsevier Inc. All rights reserved.

Enzymatic characterization of transglutaminase from Streptomyces mobaraensis DSM 40587 in high salt and effect of enzymatic cross-linking of yak milk proteins on functional properties of stirred yogurt.

PubMed

Zhang, L; Zhang, L; Yi, H; Du, M; Ma, C; Han, X; Feng, Z; Jiao, Y; Zhang, Y

2012-07-01

Streptomyces transglutaminase (TGase) purified from high-salt medium was characterized and applied into yak yogurts. The purified enzyme presented a Michaelis constant of 40.47 mmol and a maximum velocity of 44.44 U/mg of protein for N-carboxybenzoyl-l-glutaminyl-glycine in the hydroxamate procedure. The purified TGase exhibited optimum activity at 55°C and pH 6.0. The enzyme was not stable above 50°C and was stable within a pH range of 5.0 to 10.0 at 4°C for 12h and pH 5.0 to 9.0 at 37°C for 30 min. The TGase activity was not affected by Ca(2+), K(+), Ba(2+), or Na(+), but slightly inhibited by Fe(2+), Mg(2+), and Mn(2+), and strongly by Cu(2+) and Zn(2+). To explore yak milk products, it was used to produce yogurt and TGase was used. It was found that TGase-catalyzed cross-linking was effective in improving functional properties of stirred yak yogurt. Treated yogurt produced a strong acid gel, higher consistency, cohesiveness, index of viscosity, and creamier mouth feel than the untreated product. Furthermore, yak yogurt treated with TGase presented lower wet yak hair or sweat odor, or both. Therefore, TGase can be used to pave the way for exploration of novel yak products to overcome the issues of peculiar wet yak hair or sweat odor, or both. Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Yak IGF2 Promotes Fibroblast Proliferation Via Suppression of IGF1R and PI3KCG Expression

PubMed Central

Wang, Qi; Gong, Jishang; Du, Jiaxing; Zhang, Yong; Zhao, Xingxu

2018-01-01

Insulin-like growth factor 2 (IGF2) recapitulates many of the activities of insulin and promotes differentiation of myoblasts and osteoblasts, which likely contribute to genetic variations of growth potential. However, little is known about the functions and signaling properties of IGF2 variants in yaks. The over-expression vector and knockdown sequence of yak IGF2 were transfected into yak fibroblasts, and the effects were detected by a series of assays. IGF2 expression in yak muscle tissues was significantly lower than that of other tissues. In yak fibroblasts, the up-regulated expression of IGF2 inhibits expression of IGF1 and insulin-like growth factor 2 receptor (IGF2R) and significantly up-regulates expression of IGF1R. Inhibition of IGF2 expression caused the up-regulates expression of IGF1, IGF1R and IGF2R. Both over-expression and knockdown of IGF2 resulted in up-regulation of threonine protein kinase 1 (Akt1) expression and down-regulation of phosphatidylinositol 3-kinase, catalytic subunit gamma (PIK3CG). Cell cycle and cell proliferation assays revealed that over-expression of IGF2 enhanced the DNA synthesis phase and promoted yak fibroblasts proliferation. Conversely, knockdown of IGF2 decreased DNA synthesis and inhibited proliferation. These results suggested that IGF2 was negatively correlated with IGF1R and PIK3CG and demonstrated an association with the IGFs-PI3K-Akt (IGFs-phosphatidylinositol 3-kinase- threonine protein kinase) pathway in cell proliferation and provided evidence supporting the functional role of IGF2 for use in improving the production performance of yaks. PMID:29558395

Changes in the Anatomic and Microscopic Structure and the Expression of HIF-1α and VEGF of the Yak Heart with Aging and Hypoxia

PubMed Central

He, Yanyu; Yu, Sijiu; Hu, Junwei; Cui, Yan; Liu, Penggang

2016-01-01

The study aimed to identify the changes of anatomic and microscopic structure and the expression and localization of hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) in the myocardium and coronary artery of the yak heart adapted to chronic hypoxia with aging. Thirty-two yaks (1 day, 6 months, 1 year, 2 years, and 5 year old) were included, and immunoelectronmicroscopy, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) were used. Right ventricular hypertrophy was not present in yaks with aging. There was no intima thickening phenomenon in the coronary artery. The ultrastructure of myofibrils, mitochondria, and collagen fibers and the diameter and quantity of collagen changed significantly with aging. The enzymatic activity of complexes I, II, and V increased with age. Immunogold labeling showed the localization of HIF-1α protein in the cytoplasm and nuclei of endothelial cells and cytoplasm of cardiac muscle cells, and VEGF protein in the nuclei and perinuclei areas of smooth muscle cells of coronary artery, and in the cytoplasm and nuclei of endothelial cells. ELISA results showed that HIF-1α secretion significantly increased in the myocardium and coronary artery from an age of 1 day to 2 years of yaks and decreased in old yaks. However, VEGF protein always increased with aging. The findings of this study suggest that 6 months is a key age of yak before which there are some adaptive changes to deal with low-oxygen environment, and there is a maturation of the yak heart from the age of 6 months to 2 years. PMID:26914488

The Chemical Composition and Nitrogen Distribution of Chinese Yak (Maiwa) Milk

PubMed Central

Li, Haimei; Ma, Ying; Li, Qiming; Wang, Jiaqi; Cheng, Jinju; Xue, Jun; Shi, John

2011-01-01

The paper surveyed the chemical composition and nitrogen distribution of Maiwa yak milk, and compared the results with reference composition of cow milk. Compared to cow milk, yak milk was richer in protein (especially whey protein), essential amino acids, fat, lactose and minerals (except phosphorus). The contents of some nutrients (total protein, lactose, essential amino acids and casein) were higher in the warm season than in the cold season. Higher ratios of total essential amino acids/total amino acids (TEAA/TAA) and total essential amino acids/total non essential amino acids (TEAA/TNEAA) were found in the yak milk from the warm season. However its annual average ratio of EAA/TAA and that of EAA/NEAA were similar to those of cow milk. Yak milk was rich in calcium and iron (p < 0.05), and thus may serve as a nutritional ingredient with a potential application in industrial processing. PMID:21954332

Genetic diversity and classification of Tibetan yak populations based on the mtDNA COIII gene.

PubMed

Song, Q Q; Chai, Z X; Xin, J W; Zhao, S J; Ji, Q M; Zhang, C F; Ma, Z J; Zhong, J C

2015-03-13

To determine the level of genetic diversity and phylogenetic relationships among Tibetan yak populations, the mitochondrial DNA cytochrome c oxidase subunit 3 (COIII) genes of 378 yak individuals from 16 populations were analyzed in this study. The results showed that the length of cytochrome c oxidase subunit 3 gene sequences was 781 bp, with nucleotide frequencies of 29.2, 29.4, 26.1, and 15.2% for T, C, A, and G, respectively. A total of 26 haplotypes were identified, with 69 polymorphic sites, including 11 parsimony-informative sites and 58 single-nucleotide polymorphism sites. No deletions/insertions were found in sequence comparison, indicating that nucleotide mutation types were transitions and transversions. Haplotype and nucleotide diversities were 0.562 and 0.00138, respectively, indicating a high level of genetic diversity in Tibetan yak populations. Phylogenetic relationship analysis indicated that Tibetan yak populations are divided into 2 groups.

Flavor evaluation of yak butter in Tsinghai-Tibet Plateau and isolation of microorganisms contributing flavor.

PubMed

Hu, SongQing; Wei, HaiLiu; Guo, ShaSha; Li, Lin; Hou, Yi

2011-02-01

Yak butter in Tsinghai-Tibet Plateau possesses the characters of high energy, abundant alimentation and a special flavor with certain medical and health care functions. In this paper the organoleptic flavor of yak butter was estimated, and 28 kinds of substance with different flavors were identified with the technique of coupling gas chromatography to mass spectroscopy (GC-MS). The results showed that there are many microorganisms in yak butter with natural inoculation, which contribute to the formation of its special flavors. It was found that three of these 15 microorganisms, identified as Saccharomycetaceae, Penicillium and Asperillus separately, contributed the most to flavors. The microorganisms are expected to be applied in the food industry, especially to produce dairy food with the unique flavor of yak butter. © 2010 The Authors. Journal compilation © 2010 Japanese Society of Animal Science.

The effects of single nucleotide polymorphisms (SNPs) of calpastatin (CAST) gene on meat tenderness of yak.

USDA-ARS?s Scientific Manuscript database

The association of single nucleotide polymorphisms (SNPs) of calpastatin (CAST) gene with shear force of 2.54 cm steaks from M. longissimus dorsi from Gannan yaks (Bos grunniens, n=181) was studied. Yaks were harvested at 2, 3, and 4 yr of age (n=51, 59, and 71, respectively), and samples of each ya...

Quantitative proteomic analysis of whey proteins in the colostrum and mature milk of yak (Bos grunniens).

PubMed

Yang, Yongxin; Zhao, Xiaowei; Yu, Shumin; Cao, Suizhong

2015-02-01

Yak (Bos grunniens) is an important natural resource in mountainous regions. To date, few studies have addressed the differences in the protein profiles of yak colostrum and milk. We used quantitative proteomics to compare the protein profiles of whey from yak colostrum and milk. Milk samples were collected from 21 yaks after calving (1 and 28 d). Whey protein profiles were generated through isobaric tag for relative and absolute quantification (iTRAQ)-labelled proteomics. We identified 183 proteins in milk whey; of these, the expression levels of 86 proteins differed significantly between the whey from colostrum and milk. Haemoglobin expression showed the greatest change; its levels were significantly higher in the whey from colostrum than in mature milk whey. Functional analysis revealed that many of the differentially expressed proteins were associated with biological regulation and response to stimuli. Further, eight differentially expressed proteins involved in the complement and coagulation cascade pathway were enriched in milk whey. These findings add to the general understanding of the protein composition of yak milk, suggest potential functions of the differentially expressed proteins, and provide novel information on the role of colostral components in calf survival. © 2014 Society of Chemical Industry.

Effects of Yak grazing on plant community characteristics of meadow grasslands in the Qinghai-Tibetan Plateau, China

NASA Astrophysics Data System (ADS)

Chai, Linrong; Hou, Fujiang; Bowatte, Saman; Cheng, Yunxiang

2017-04-01

The Qinghai-Tibetan Plateau (QTP) is an important part of the global terrestrial ecosystem that provides many ecological roles such as biodiversity protection, upper watersheds for large rivers, circulation of materials, energy balance and provision of forage and habitat for livestock and wildlife. Approximately 40% of the QTP is alpine meadow grasslands and yak farming is one of the dominant land use activities. In recent years, the rapid increase in the number of yaks grazing on meadow grasslands has raised concerns about grassland degradation. In this study we examined the effects of yak grazing on the grassland community characteristics to evaluate the degradation potential of alpine meadow in the QTP. The experiment was carried out on three farms, in close proximity to each other, that were operating at different grazing intensities in Maqu county (N35°58', E101°53', altitude 3650m) of the QTP in the Gansu province in China. We tested 4 levels of yak grazing intensities; control (no grazing), light (2.6yak/ha), moderate (3.5yak/ha) and high (6.5yak/ha ). We hypothesized that greater intensity of grazing would significantly impact the plant community characteristics through trampling effects above and below ground. We found grazing significantly (P<0.05) impacted the above and below ground biomass. Above ground biomass was highest in the non grazed area and lowest in the high grazing farm. In contrast, below ground biomass was significantly greater (p<0.05) in the moderate grazing farm compared to the non grazed area. The plant community density and the proportion of edible grass biomass were not significantly affected by the grazing treatments. The species richness was significantly reduced (p<0.05) in the moderate and high intensity grazing farms compared to the non grazed area. The soil moisture at 0-10 cm depth was significantly lower at the high grazing intensity farm compared to the non grazed area and in contrast soil temperature was significantly higher. Our results indicate that increasing the yak grazing pressure affected the plant community characteristics in alpine meadow in the QTP, indicating a potential for grassland degradation to occur in future. Therefore, further comprehensive research is warranted. Key words: Qinghai-Tibetan Plateau; Yak grazing; Alpine meadow; Maqu county

Genetic polymorphisms of the IGF-II gene intron 8 coding region and its association with growth and carcass traits in yak.

PubMed

Zeng, Y F; Ding, X Z; Cheng, S R; Yu, S J

2013-12-11

Insulin-like growth factor II (IGF-II) plays a key role in mammalian growth and is involved in stimulating fetal cell division, differentiation, and metabolic regulation. IGF-II is considered a candidate gene for genetic markers of growth and carcass traits. Therefore, in this study, the associations of single nucleotide polymorphisms (SNPs) in the IGF-II gene region with growth and carcass characteristics in five yak breeds were investigated. Two SNPs, G(330)C and A(358)G, were identified by sequencing intron 8 of the IGF-II gene in homozygotes. Two alleles, A and B, and three genotypes, AA, AB, and BB, were identified by polymerase chain reaction. Genotypic frequencies of IGF-II allele B were 0.8623, 0.8936, 0.8535, 0.8676, and 0.8300 for Datong yak, Gannan yak, Tianzhu white yak, Qinghai Plateau yak, and Xinjiang yak, respectively. Allele and the genotype of IGF-II were strongly associated with growth and carcass traits. Least square analysis revealed a significant effect (P < 0.01) of genotypes AA and AB compared with genotype BB on live-weight (at 12, 13-24, and 25-36 months of age), average daily weight gain (P < 0.01) and carcass weight (P < 0.05). Animals with genotype AB had a higher mean rib eye area, and a lower mean yield grade. The results indicated that the IGF-II gene acts by a primarily additive biological mechanism by adding weight independently of skeletal growth.

Serological survey of Toxoplasma gondii in Tibetan mastiffs (Canis lupus familiaris) and yaks (Bos grunniens) in Qinghai, China

PubMed Central

2012-01-01

Background Toxoplasma gondii is an amphixenosis which has extensive hosts. In recent years, the prevalence of T. gondii in China has been reported, while little is known on the survey of T. gondii infection in northwest China, especially in yaks (Bos grunniens) and Tibetan mastiffs (Canis lupus familiaris). The current study survey the infection of T. gondii in Tibetan mastiffs and yaks in Qinghai Province, China. Methods The indirect hemagglutination test (IHAT) was used to examine T. gondii antibodies in 1 795 serums, including 192 Tibetan mastiffs and 1603 yaks in Qinghai Province, China. Results In this study, the seroprevalence of T. gondii infection was 8.52%. Twenty (10.42%) of 192 serums of Tibetan mastiffs and 133 (8.30%) of 1603 serums of yaks were seropositive. The seroprevalence of T.gondii infection in Tibetan mastiffs in breeding farm (1.08%) was lower than that in the field (19.19%), and the difference was statistically significant (P <0.05). The seroprevalence of antibodies to T.gondii in yaks ranged from 5.45% to 13.28% among the four different areas. The seroprevalence in different age groups were determined with apparent association. Conclusions The results indicated that T.gondii infection was prevalent in Tibetan mastiffs and yaks, which have implications for public health in this region. To our knowledge, this is the first seroprevalence survey of Tibetan mastiffs infected by T. gondii in The People’s Republic of China. PMID:22330277

Triallelic SNPs for estimating cattle introgression, inbreeding, and determining parentage in North American yak

USDA-ARS?s Scientific Manuscript database

The current population of yaks in the U.S. is estimated to exceed 5,000 and was derived from about 150 animals imported in the 20th Century. During the expansion of the U.S. herd, some yaks were allowed to hybridize with cattle, although it is not clear to what extent. Our aim was to use next genera...

Origin of mitochondrial DNA diversity of domestic yaks

PubMed Central

Guo, Songchang; Savolainen, Peter; Su, Jianping; Zhang, Qian; Qi, Delin; Zhou, Jie; Zhong, Yang; Zhao, Xinquan; Liu, Jianquan

2006-01-01

Background The domestication of plants and animals was extremely important anthropologically. Previous studies have revealed a general tendency for populations of livestock species to include deeply divergent maternal lineages, indicating that they were domesticated in multiple, independent events from genetically discrete wild populations. However, in water buffalo, there are suggestions that a similar deep maternal bifurcation may have originated from a single population. These hypotheses have rarely been rigorously tested because of a lack of sufficient wild samples. To investigate the origin of the domestic yak (Poephagus grunnies), we analyzed 637 bp of maternal inherited mtDNA from 13 wild yaks (including eight wild yaks from a small population in west Qinghai) and 250 domesticated yaks from major herding regions. Results The domestic yak populations had two deeply divergent phylogenetic groups with a divergence time of > 100,000 yrs BP. We here show that haplotypes clustering with two deeply divergent maternal lineages in domesticated yaks occur in a single, small, wild population. This finding suggests that all domestic yaks are derived from a single wild gene pool. However, there is no clear correlation of the mtDNA phylogenetic clades and the 10 morphological types of sampled yaks indicating that the latter diversified recently. Relatively high diversity was found in Qinghai and Tibet around the current wild distribution, in accordance with previous suggestions that the earliest domestications occurred in this region. Conventional molecular clock estimation led to an unrealistic early dating of the start of the domestication. However, Bayesian estimation of the coalescence time allowing a relaxation of the mutation rate are better in agreement with a domestication during the Holocene as supported by archeological records. Conclusion The information gathered here and the previous studies of other animals show that the demographic histories of domestication of livestock species were highly diverse despite the common general feature of deeply divergent maternal lineages. The results further suggest that domestication of local wild prey ungulate animals was a common occurrence during the development of human civilization following the postglacial colonization in different locations of the world, including the high, arid Qinghai-Tibetan Plateau. PMID:16995938

Yik Yak: a social media sensor

NASA Astrophysics Data System (ADS)

Leskovich, W. Robert

2015-05-01

This is the first academic paper which focuses specifically on the new social media application Yik Yak. To provide a solid foundation, a brief overview of a few anonymous social media platforms is provided. A social media sensor framework is then presented which utilizes a three-layered approach to addressing the use of analytic tools. Specifically the use of keyword, geolocation, sentiment, and network analysis is explored through the perspective of social media as a sensor. Challenges and criticisms are exposed in addition to some possible solutions. A theoretical case study is then offered which outlines a potential use of social media as a senor for emergency managers. The paper culminates with a data collection for the development of a lexicon for Yik Yak. This data collection focuses on an 18 day study which collects Yik Yak posts and Twitter tweets simultaneously. The top 100 keywords for each platform are collected for every 24 hour period and placed through a relative change comparison. Overall, Yik Yak offers a more stable baseline as compared to Twitter.

Differences in proteomic profiles of milk fat globule membrane in yak and cow milk.

PubMed

Ji, Xiaoxi; Li, Xisheng; Ma, Ying; Li, Day

2017-04-15

Milk fat globule membrane (MFGM) is an important milk component which is rich in bioactive proteins. In this work, the isobaric tags for relative and absolute quantitation (iTRAQ) proteomic approach was used to investigate the differences in the MFGM proteins between yak and cow milk. Over 450 proteins were identified between the yak and cow MFGM. The MFGM proteins with significant differences were compared based on the relative abundance. Proteins such as Glycosylation-dependent cell adhesion molecule 1 (GlyCAM1), CD59 molecule and lactadherin, were identified having a much higher abundance (4.6-10.1 fold) in yak MFGM than cow MFGM. These proteins are thought to have biological functions such as the antimicrobial and antitumor effects. This may be due to the need that yak produces high nutritive milk including high levels of bioactive compounds in order to resist the extreme high altitude environment. Copyright © 2016 Elsevier Ltd. All rights reserved.

Developing a Framework for Control of Agile Aircraft Platforms in Autonomous Hover

DTIC Science & Technology

2009-03-01

profiles. Two dynamical systems are considered, a scale YAK -54 aerobatic remote control aircraft and the Flexrotor concept developed by Aerovel. Both models...System [28]. . . . . . . 2 1.2 A YAK -54 in hover in the Real Flight RC Simulator [24]. . . . . . . . 3 1.3 The Aerovel Flexrotor concept...17 3.1 A three-view of the YAK -54 showing all geometry and dimensions (in mm) [15

Constraining Source Terms, Regional Attenuation Models, and Site Effects (Postprint)

DTIC Science & Technology

2012-03-22

YAK ZRNK 10245 10831 11116 11346 12278...MDJ KAR NIL PDG QIZ RAYN SSE TATO TKM2TLG TLY UCHULHL ULN USP VOS WMQ WUS XAN YAK ZRNK 10245 10831 11116 11346 12278 12376 12574 12937 1311713130...LSA LZH MAKZUZ MDJ KAR NIL PDG QIZ RAYN SSE TATO TKM2TLG TLY UCHULHL ULN USP VOS WMQ WUS XAN YAK ZRNK 10245 10831 11116 11346 12278 12376 12574

A CASE OF THEILERIOSIS IN THE TIBETIAN YAK (P. GRUNNEUS),

DTIC Science & Technology

Smears taken from the dead and recovered yaks were coloured by Romanovsky-Giemsa and Leishman method but the Theileria of Tibetian yaks proved to be...different from Theileria annulata; in smears rodlike, anaplasmoid, commashaped, and punctiform; the round or ringshaped forms that predominate in... Theileria annalata were very rarely found. Pearshaped forms were not revealed in smears. The parasites are mononuclear. The nucleus is located in one

Comparative Proteomic Analysis of Yak Follicular Fluid during Estrus

PubMed Central

Guo, Xian; Pei, Jie; Ding, Xuezhi; Chu, Min; Bao, Pengjia; Wu, Xiaoyun; Liang, Chunnian; Yan, Ping

2016-01-01

The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification. The expression level of transferrin and enolase superfamily member 1 (ENOSF1) was determined by Western blotting for verification analysis. The results showed that 2-DE obtained an electrophoresis map of proteins from mature and immature yak FF with high resolution and repeatability. A comparison of protein profiles identified 12 differently expressed proteins, out of which 10 of them were upregulated while 2 were downregulated. Western blotting showed that the expression of transferrin and ENOSF1 was enhanced with follicular development. Both the obtained protein profiles and the differently expressed proteins identified in this study provided experimental data related to follicular development during yak breeding seasons. This study also laid the foundation for understanding the microenvironment during oocyte development. PMID:26954118

Sequence Characterization of the MC1R Gene in Yak (Poephagus grunniens) Breeds with Different Coat Colors

PubMed Central

Chen, Shi-Yi; Huang, Yi; Zhu, Qing; Fontanesi, Luca; Yao, Yong-Gang; Liu, Yi-Ping

2009-01-01

Melanocortin 1 receptor (MC1R) gene plays a key role in determining coat color in several species, including the cattle. However, up to now there is no report regarding the MC1R gene and the potential association of its mutations with coat colors in yak (Poephagus grunniens). In this study, we sequenced the encoding region of the MC1R gene in three yak breeds with completely white (Tianzhu breed) or black coat color (Jiulong and Maiwa breeds). The predicted coding region of the yak MC1R gene resulted of 954 bp, the same to that of the wild-type cattle sequence, with >99% identity. None of the mutation events reported in cattle was found. Comparing the yak obtained sequences, five nucleotide substitutions were detected, which defined three haplotypes (EY1, EY2, and EY3). Of the five mutations, two, characterizing the EY1 haplotype, were nonsynonymous substitutions (c.340C>A and c.871G>A) causing amino acid changes located in the first extracellular loop (p.Q114K) and in the seventh transmembrane region (p.A291T). In silico prediction might indicate a functional effect of the latter substitution. However, all three haplotypes were present in the three yak breeds with relatively consistent frequency distribution, despite of their distinguished coat colors, which suggested that there was no across-breed association between haplotypes or genotypes and black/white phenotypes, at least in the investigated breeds. Other genes may be involved in affecting coat color in the analyzed yaks. PMID:19584942

Characterization of transcriptional complexity during pre-implantation development of the yak (Bos grunniens) using RNA-Seq.

PubMed

Zi, X-D; Luo, B; Xia, W; Zheng, Y-C; Xiong, X-R; Li, J; Zhong, J-C; Zhu, J-J; Zhang, Z-F

2018-06-01

The objective of this study was to investigate the mechanism that regulates pre-implantation development of the yak (Bos grunniens). We determined the transcriptomes of in vitro-produced yak embryos at two-cell, four-cell, eight-cell stages, and morula and blastocyst using the Illumina RNA-seq for the first time. We obtained 47.36-50.86 million clean reads for each stage, of which, 85.65%-90.02% reads were covered in the reference genome. A total of 17,368 genes were expressed during the two-cell stage to blastocyst of the yak, of which 7,236 genes were co-expressed at all stages, whereas 10,132 genes were stage-specific expression. Transcripts from 9,827 to 14,893 different genes were detected in various developmental stages. When |log 2 ratio| ≥ 1 and q-value <0.05 were set as thresholds for identifying differentially expressed genes (DEGs), we detected a total of 6,922-10,555 DEGs between any two consecutive stages. The GO distributions of these DEGs were classified into three categories: biological processes (23 terms), cellular components (22 terms) and molecular functions (22 terms). Pathway analysis revealed 310 pathways of the DEGs that were operative in early pre-implantation yak development, of which 32 were the significantly enriched pathways. In conclusion, this is the first report to investigate the mechanism that regulates yak embryonic development using high-throughput sequencing, which provides a comprehensive framework of transcriptome landscapes of yak pre-implantation embryos. © 2018 Blackwell Verlag GmbH.

Molecular characterization, mRNA expression of prolactin receptor (PRLR) gene during pregnancy, nonpregnancy in the yak (Bos grunniens).

PubMed

Zi, Xiang-Dong; Chen, Da-Wen; Wang, Hong-Mei

2012-02-01

Prolactin (PRL) plays central roles in a wide range of body functions in mammals, and the actions are mediated by the specific cell surface receptor, the prolactin receptor (PRLR). To better understand the role of PRL in the yak (Bos grunniens), in the present study, we first cloned yak PRLR cDNA, and compared its mRNA expression in several tissues with cattle (Bos taurus). By reverse transcriptase-polymerase chain reaction (RT-PCR) strategy, we obtained full-length of yak PRLR cDNA sequence comprised of an open reading frame of 1746bp encoding a 581 amino acid protein, and contained a signal sequence and a transmembrane region. The intracellular domain had two pairs of cysteine residues and a WSXWS motif. The cytoplasmic domain comprised 323 residues and contained box 1 sequence. The yak PRLR shared 66.0-98.5% protein sequence identity with mammalian homologs. Real-time PCR analysis revealed that PRLR mRNA was higher in mammary tissue than in ovary and endometrium (P<0.01). During pregnancy, the ovary and mammary PRLR mRNA expression increased by 33- and 2.9-fold in yak, respectively, and increased by 46- and 3.8-fold in cattle, respectively. PRLR mRNA expression was higher (P<0.05) in mammary tissue and ovary of pregnant cow than that of pregnant yak. It is proposed that the increased ovarian and mammary PRLR mRNA expression during pregnancy may be associated with corpus luteum function for maintenance of pregnancy and mammary development for subsequent lactation. Copyright © 2011 Elsevier Inc. All rights reserved.

Oocyte extract improves epigenetic reprogramming of yak fibroblast cells and cloned embryo development.

PubMed

Xiong, X R; Li, J; Fu, M; Gao, C; Wang, Y; Zhong, J C

2013-02-01

The objective was to investigate the effects of bovine oocyte extract (BOE) on epigenetic reprogramming of yak fibroblast cells, based on their cell cycle status, histone acetylation, DNA methylation, gene expression, and cloned blastocyst formation. Permeabilization of yak fibroblasts after treatment with 10 or 50 μL of BOE (treated-S and treated-L groups, respectively) for 24 hours increased (P < 0.05) the cell population at the G(0)/G(1) phase (85.2 ± 2.3% and 89.6 ± 1.5%, respectively) compared with controls (75.4 ± 1.1%). Acetylation at lysine 9 of histone H3 was also higher (26.1 ± 1.4 and 33.5 ± 2.1) than in the control group (15.3 ± 1.6; P < 0.05). Moreover, BOE reduced methylation of the promoter regions of Oct-4 and Nanog (76.4% and 72.2%; and 35.6% and 30.0%, respectively) compared with the control group (92.1% and 47.8%; P < 0.05). In addition, the relative expression levels of HDAC-1, HADC-2, Dnmt-1, and Dnmt-3a were downregulated (P < 0.05) after yak fibroblasts were treated with BOE. Furthermore, when yak fibroblasts were used for interspecies somatic cell nuclear transfer after BOE treatment, 8-cell and blastocyst formation rates significantly exceeded those of the control. In conclusion, BOE induced epigenetic reprogramming of yak fibroblasts, making them suitable donors for yak interspecies somatic cell nuclear transfer. Crown Copyright © 2013. Published by Elsevier Inc. All rights reserved.

Crustal Structure of the Yakutat Microplate: New Constraints for Understanding the Evolution of Subduction and Collision in southern Alaska

NASA Astrophysics Data System (ADS)

Worthington, L. L.; van Avendonk, H. J.; Gulick, S. P.; Christeson, G. L.; Pavlis, T. L.

2010-12-01

Flat-slab subduction and accretion of the Yakutat (YAK) microplate in southern Alaska characterizes the most recent iteration in the process of terrane accretion that has built the tectonic assemblage of the Canada-Alaska Cordillera since the Mesozoic. Despite the potentially pivotal role of the Yakutat collision in the evolution and deformation of the North American Cordillera, major questions regarding locations of active faults and velocity structure and thickness of the Yakutat block itself have gone unanswered. We present results of a 2008 marine seismic reflection/refraction survey acquired as part of the St. Elias Erosion and Tectonics Project (STEEP), a multi-disciplinary NSF-Continental Dynamics project aimed at structural evolution and geodynamics related to the YAK collision. An onshore-offshore wide-angle refraction profile shows YAK crustal thickness ranging from ~15 km near the Bering Glacier to ~35 km east of the Dangerous River Zone (DRZ), with calculated lower crustal velocities potentially >7km/s. Crustal velocity and structure are continuous across the DRZ on the YAK shelf, which is historically described as a vertical boundary between continental crust on the east and oceanic basement on the west. Instead, we observe a gradual shallowing of elevated crustal velocities associated with a basement high observed on coincident marine reflection data near the DRZ. Crustal velocity and thicknesses are comparable to the Kerguelen oceanic plateau and the Siletz terrane, thus supporting the oceanic plateau theory for the origin of the YAK microplate. The observed variable crustal thickness indicates that the YAK slab may be slightly wedge-shaped, thinning in the direction of subduction. The thickest portion of the offshore YAK is entering the orogen near the eastern syntaxis, where the Fairweather fault system encounters a restraining bend as its orientation changes from north-south to east-west. It follows that observations of elevated exhumation rates and concentrated seismicity in the vicinity of the syntaxis may not be the exclusive result of this corner geometry. Instead, we must consider that underlying crustal structure of the YAK indentor partially determines the large-scale patterns of mountain building in southern Alaska. These observations also imply that uplift and deformation have intensified through time as thicker, more buoyant YAK crust attempts to subduct.

Evaluation of foot and mouth vaccination for yak (Bos grunniens) in Pakistan.

PubMed

Mortenson, J A; Khan, E H Haq; Ali, I; Manzoor, S; Jamil, A; Abubakar, M; Afzal, M; Hussain, M

2017-04-01

In northern Pakistan, many farming communities rely on domestic yak (Bos grunniens) as a principle source of income. A 2006 participatory disease surveillance report from this region indicated that foot-and-mouth disease (FMD) is the most prevalent annual disease of yak. Our objectives of this study were to determine exposure levels of yak to FMD virus; implement a vaccination program based on current, regional FMD virus serotypes and subtypes; and quantify immune responses following vaccination. Blood samples were used to determine pre-vaccination exposure of animals to FMD virus by antibody presence to non-structural proteins of FMD virus using a 3-ABC trapping indirect ELISA. Vaccine used consisted of FMD serotypes 'O' (PanAsia-2), 'A' (Iran-05), and 'Asia-1' (Shamir), but changed later during the study to match newly circulating viruses in the country ('O'-PanAsia-2; 'A'-Turk-06 and Asia-1-Sindh-08). Three hundred sixty-three blood samples were tested from selected villages to determine pre-vaccination FMD virus exposure in yak with an average of 37.7%. Immune responses from initial vaccination and booster dose 30 days later showed clear protective levels (as mean percent inhibition) of antibodies against structural proteins of serotypes 'O,' 'A,' and 'Asia-1.' These responses remained above threshold positive level even at day 210 following initial vaccination. Results of sero-surveillance and anecdotal information of repeated FMD outbreaks demonstrate the persistence of FMD virus of yak in northern Pakistan. Laboratory results and field observations clearly indicated that yak can be protected against FMD with a good quality vaccine with FMD serotype(s) matching current, regionally circulating FMD virus.

Effect of DNA Extraction Methods on the Apparent Structure of Yak Rumen Microbial Communities as Revealed by 16S rDNA Sequencing.

PubMed

Chen, Ya-Bing; Lan, Dao-Liang; Tang, Cheng; Yang, Xiao-Nong; Li, Jian

2015-01-01

To more efficiently identify the microbial community of the yak rumen, the standardization of DNA extraction is key to ensure fidelity while studying environmental microbial communities. In this study, we systematically compared the efficiency of several extraction methods based on DNA yield, purity, and 16S rDNA sequencing to determine the optimal DNA extraction methods whose DNA products reflect complete bacterial communities. The results indicate that method 6 (hexadecyltrimethylammomium bromide-lysozyme-physical lysis by bead beating) is recommended for the DNA isolation of the rumen microbial community due to its high yield, operational taxonomic unit, bacterial diversity, and excellent cell-breaking capability. The results also indicate that the bead-beating step is necessary to effectively break down the cell walls of all of the microbes, especially Gram-positive bacteria. Another aim of this study was to preliminarily analyze the bacterial community via 16S rDNA sequencing. The microbial community spanned approximately 21 phyla, 35 classes, 75 families, and 112 genera. A comparative analysis showed some variations in the microbial community between yaks and cattle that may be attributed to diet and environmental differences. Interestingly, numerous uncultured or unclassified bacteria were found in yak rumen, suggesting that further research is required to determine the specific functional and ecological roles of these bacteria in yak rumen. In summary, the investigation of the optimal DNA extraction methods and the preliminary evaluation of the bacterial community composition of yak rumen support further identification of the specificity of the rumen microbial community in yak and the discovery of distinct gene resources.

Proteomic analysis of cow, yak, buffalo, goat and camel milk whey proteins: quantitative differential expression patterns.

PubMed

Yang, Yongxin; Bu, Dengpan; Zhao, Xiaowei; Sun, Peng; Wang, Jiaqi; Zhou, Lingyun

2013-04-05

To aid in unraveling diverse genetic and biological unknowns, a proteomic approach was used to analyze the whey proteome in cow, yak, buffalo, goat, and camel milk based on the isobaric tag for relative and absolute quantification (iTRAQ) techniques. This analysis is the first to produce proteomic data for the milk from the above-mentioned animal species: 211 proteins have been identified and 113 proteins have been categorized according to molecular function, cellular components, and biological processes based on gene ontology annotation. The results of principal component analysis showed significant differences in proteomic patterns among goat, camel, cow, buffalo, and yak milk. Furthermore, 177 differentially expressed proteins were submitted to advanced hierarchical clustering. The resulting clustering pattern included three major sample clusters: (1) cow, buffalo, and yak milk; (2) goat, cow, buffalo, and yak milk; and (3) camel milk. Certain proteins were chosen as characterization traits for a given species: whey acidic protein and quinone oxidoreductase for camel milk, biglycan for goat milk, uncharacterized protein (Accession Number: F1MK50 ) for yak milk, clusterin for buffalo milk, and primary amine oxidase for cow milk. These results help reveal the quantitative milk whey proteome pattern for analyzed species. This provides information for evaluating adulteration of specific specie milk and may provide potential directions for application of specific milk protein production based on physiological differences among animal species.

Identification and verification of potential piRNAs from domesticated yak testis.

PubMed

Gong, Jishang; Zhang, Quanwei; Wang, Qi; Ma, Youji; Du, Jiaxiang; Zhang, Yong; Zhao, Xingxu

2018-02-01

PIWI-interacting RNAs (piRNA) are small non-coding RNA molecules expressed in animal germ cells that interact with PIWI family proteins to form RNA-protein complexes involved in epigenetic and post-transcriptional gene silencing of retrotransposons and other genetic elements in germ line cells, including reproductive stem cell self-sustainment, differentiation, meiosis and spermatogenesis. In the present study, we performed high-throughput sequencing of piRNAs in testis samples from yaks in different stages of sexual maturity. Deep sequencing of the small RNAs (18-40 nt in length) yielded 4,900,538 unique reads from a total of 53,035,635 reads. We identified yak small RNAs (18-30 nt) and performed functional characterization. Yak small RNAs showed a bimodal length distribution, with two peaks at 22 nt and >28 nt. More than 80% of the 3,106,033 putative piRNAs were mapped to 4637 piRNA-producing genomic clusters using RPKM. 6388 candidate piRNAs were identified from clean reads and the annotations were compared with the yak reference genome repeat region. Integrated network analysis suggested that some differentially expressed genes were involved in spermatogenesis through ECM-receptor interaction and PI3K-Akt signaling pathways. Our data provide novel insights into the molecular expression and regulation similarities and diversities in spermatogenesis and testicular development in yaks at different stages of sexual maturity. © 2018 The authors.

Molecular Cloning, Bioinformatics Analysis and Expression of Insulin-Like Growth Factor 2 from Tianzhu White Yak, Bos grunniens

PubMed Central

Zhang, Quanwei; Gong, Jishang; Wang, Xueying; Wu, Xiaohu; Li, Yalan; Ma, Youji; Zhang, Yong; Zhao, Xingxu

2014-01-01

The IGF family is essential for normal embryonic and postnatal development and plays important roles in the immune system, myogenesis, bone metabolism and other physiological functions, which makes the study of its structure and biological characteristics important. Tianzhu white yak (Bos grunniens) domesticated under alpine hypoxia environments, is well adapted to survive and grow against severe hypoxia and cold temperatures for extended periods. In this study, a full coding sequence of the IGF2 gene of Tianzhu white yak was amplified by reverse transcription PCR and rapid-amplification of cDNA ends (RACE) for the first time. The cDNA sequence revealed an open reading frame of 450 nucleotides, encoding a protein with 179 amino acids. Its expression in different tissues was also studied by Real time PCR. Phylogenetic tree analysis indicated that yak IGF2 was similar to Bos taurus, and 3D structure showed high similarity with the human IGF2. The putative full CDS of yak IGF2 was amplified by PCR in five tissues, and cDNA sequence analysis showed high homology to bovine IGF2. Moreover the super secondary structure prediction showed a similar 3D structure with human IGF2. Its conservation in sequence and structure has facilitated research on IGF2 and its physiological function in yak. PMID:24394317

Identification and verification of potential piRNAs from domesticated yak testis

PubMed Central

Gong, Jishang; Zhang, Quanwei; Wang, Qi; Ma, Youji; Du, Jiaxiang; Zhang, Yong

2018-01-01

PIWI-interacting RNAs (piRNA) are small non-coding RNA molecules expressed in animal germ cells that interact with PIWI family proteins to form RNA–protein complexes involved in epigenetic and post-transcriptional gene silencing of retrotransposons and other genetic elements in germ line cells, including reproductive stem cell self-sustainment, differentiation, meiosis and spermatogenesis. In the present study, we performed high-throughput sequencing of piRNAs in testis samples from yaks in different stages of sexual maturity. Deep sequencing of the small RNAs (18–40 nt in length) yielded 4,900,538 unique reads from a total of 53,035,635 reads. We identified yak small RNAs (18–30 nt) and performed functional characterization. Yak small RNAs showed a bimodal length distribution, with two peaks at 22 nt and >28 nt. More than 80% of the 3,106,033 putative piRNAs were mapped to 4637 piRNA-producing genomic clusters using RPKM. 6388 candidate piRNAs were identified from clean reads and the annotations were compared with the yak reference genome repeat region. Integrated network analysis suggested that some differentially expressed genes were involved in spermatogenesis through ECM–receptor interaction and PI3K-Akt signaling pathways. Our data provide novel insights into the molecular expression and regulation similarities and diversities in spermatogenesis and testicular development in yaks at different stages of sexual maturity. PMID:29101267

Plant dual-specificity tyrosine phosphorylation-regulated kinase optimizes light-regulated growth and development in Arabidopsis.

PubMed

Huang, Wen-Yu; Wu, Yi-Chen; Pu, Hsin-Yi; Wang, Ying; Jang, Geng-Jen; Wu, Shu-Hsing

2017-09-01

Light controls vegetative and reproductive development of plants. For a plant, sensing the light input properly ensures coordination with the ever-changing environment. Previously, we found that LIGHT-REGULATED WD1 (LWD1) and LWD2 regulate the circadian clock and photoperiodic flowering. Here, we identified Arabidopsis YET ANOTHER KINASE1 (AtYAK1), an evolutionarily conserved protein and a member of dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs), as an interacting protein of LWDs. Our study revealed that AtYAK1 is an important regulator for various light responses, including the circadian clock, photomorphogenesis and reproductive development. AtYAK1 could antagonize the function of LWDs in regulating the circadian clock and photoperiodic flowering. By examining phenotypes of atyak1, we found that AtYAK1 regulated light-induced period-length shortening and photomorphogenic development. Moreover, AtYAK1 mediated plant fertility especially under inferior light conditions including low light and short-day length. This study discloses a new regulator connecting environmental light to plant growth. © 2017 John Wiley & Sons Ltd.

Influence of succinylation on the conformation of yak casein micelles.

PubMed

Yang, Min; Cui, Na; Fang, Yan; Shi, Ying; Yang, Jitao; Wang, Jiangyu

2015-07-15

Succinylation modifies the physicochemical characteristics and improves the functional properties of proteins. This study assessed the effects of succinylation on the conformation of yak casein micelles with seven degree of modification. The results revealed that succinylation contributed to the dissociation of casein micelles. With the increase of succinylated degree, soluble nitrogen and minerals content increased, while casein micelle size and polydispersity index of micelles decreased. Succinylation affected the spatial conformation of yak casein micelles: turn decreased, ß-sheet and α-helix increased, and irregular structure were non-significantly affected. The intrinsic and ANS fluorescence intensity decreased and the maximum emission wavelength shifted red with increasing succinylation. Based on the results, the structure of yak casein micelles was characteristic of the sub-micelle model. Copyright © 2015 Elsevier Ltd. All rights reserved.

Effects of GHRP-2 and Cysteamine Administration on Growth Performance, Somatotropic Axis Hormone and Muscle Protein Deposition in Yaks (Bos grunniens) with Growth Retardation

PubMed Central

Hu, Rui; Wang, Zhisheng; Peng, Quanhui; Zou, Huawei; Wang, Hongze; Yu, Xiaoqiang; Jing, Xiaoping; Wang, Yixin; Cao, Binghai; Bao, Shanke; Zhang, Wenhua; Zhao, Suonan; Ji, Hanzhong; Kong, Xiangying; Niu, Quanxi

2016-01-01

The objective of this study was to investigate the effects of growth hormone-releasing peptide-2 (GHRP-2) and cysteamine (CS) administration on growth performance in yaks with growth retardation and try to elucidate its regulatory mechanisms. Trial 1, thirty-six 1-year-old Qinghai high plateau yaks (body weight 38–83.2 kg) were randomly chosen for body weight and jugular blood samples collection. The relationship between body weight and serum GHRH (P < 0.05, R = 0.45), GH (P < 0.05, R = 0.47), IGF-1 (P < 0.05, R = 0.62) was significantly correlated in yaks colonies with lighter body weights. Trial 2, fifteen 1-year-old Qinghai high plateau yaks with growth retardation (average body weight 54.8 ± 8.24 kg) were randomly selected and assigned to negative control group (NG), GHRP-2 injection group (GG) and cysteamine feeding group (CG), with 5 yaks per group. Another five 1-year-old Qinghai high plateau yaks with normal growth performance (average body weight 75.3 ± 2.43 kg) were selected as positive control group (PG). The average daily gain (ADG) of the GG and CG were significantly higher than those in the PG and NG (P < 0.05). Both GHRP-2 and CS administration significantly enhanced the myofiber diameter and area of skeletal muscle (P<0.05). GHRP-2 significantly enhanced the serum GH and IGF-1 levels (P < 0.05), and up-regulated GHR, IGF-1 and IGF-1R mRNA expression in the liver and skeletal muscle (P < 0.05), enhanced the mRNA expression of PI3K, AKt and mTOR in the skeletal muscle (P<0.05). CS significantly reduced the serum SS levels and the hypothalamus SS mRNA expression (P < 0.05), and enhanced GHR and IGF-1 mRNA expression in the liver (P < 0.05), decreased the mRNA expression of muscle atrophy F-box (Atrogin-1) and muscle ring finger 1 (MuRF1) mRNA (P < 0.05). Conclusions: Growth retardation in yaks was primarily due to somatotropic axis hormones secretion deficiency. Both GHRP-2 and CS administration can accelerate growth performance and GH, IGF-1 secretion in yaks with growth retardation. GHRP-2 enhanced muscle protein deposition mainly by up-regulated the protein synthesis pathways, whereas CS worked mainly by down-regulated the ubiquitin-proteasome pathway. PMID:26894743

Effects of GHRP-2 and Cysteamine Administration on Growth Performance, Somatotropic Axis Hormone and Muscle Protein Deposition in Yaks (Bos grunniens) with Growth Retardation.

PubMed

Hu, Rui; Wang, Zhisheng; Peng, Quanhui; Zou, Huawei; Wang, Hongze; Yu, Xiaoqiang; Jing, Xiaoping; Wang, Yixin; Cao, Binghai; Bao, Shanke; Zhang, Wenhua; Zhao, Suonan; Ji, Hanzhong; Kong, Xiangying; Niu, Quanxi

2016-01-01

The objective of this study was to investigate the effects of growth hormone-releasing peptide-2 (GHRP-2) and cysteamine (CS) administration on growth performance in yaks with growth retardation and try to elucidate its regulatory mechanisms. Trial 1, thirty-six 1-year-old Qinghai high plateau yaks (body weight 38-83.2 kg) were randomly chosen for body weight and jugular blood samples collection. The relationship between body weight and serum GHRH (P < 0.05, R = 0.45), GH (P < 0.05, R = 0.47), IGF-1 (P < 0.05, R = 0.62) was significantly correlated in yaks colonies with lighter body weights. Trial 2, fifteen 1-year-old Qinghai high plateau yaks with growth retardation (average body weight 54.8 ± 8.24 kg) were randomly selected and assigned to negative control group (NG), GHRP-2 injection group (GG) and cysteamine feeding group (CG), with 5 yaks per group. Another five 1-year-old Qinghai high plateau yaks with normal growth performance (average body weight 75.3 ± 2.43 kg) were selected as positive control group (PG). The average daily gain (ADG) of the GG and CG were significantly higher than those in the PG and NG (P < 0.05). Both GHRP-2 and CS administration significantly enhanced the myofiber diameter and area of skeletal muscle (P<0.05). GHRP-2 significantly enhanced the serum GH and IGF-1 levels (P < 0.05), and up-regulated GHR, IGF-1 and IGF-1R mRNA expression in the liver and skeletal muscle (P < 0.05), enhanced the mRNA expression of PI3K, AKt and mTOR in the skeletal muscle (P<0.05). CS significantly reduced the serum SS levels and the hypothalamus SS mRNA expression (P < 0.05), and enhanced GHR and IGF-1 mRNA expression in the liver (P < 0.05), decreased the mRNA expression of muscle atrophy F-box (Atrogin-1) and muscle ring finger 1 (MuRF1) mRNA (P < 0.05). Growth retardation in yaks was primarily due to somatotropic axis hormones secretion deficiency. Both GHRP-2 and CS administration can accelerate growth performance and GH, IGF-1 secretion in yaks with growth retardation. GHRP-2 enhanced muscle protein deposition mainly by up-regulated the protein synthesis pathways, whereas CS worked mainly by down-regulated the ubiquitin-proteasome pathway.

Cloning of oligopeptide transport carrier PepT1 and comparative analysis of PepT1 messenger ribonucleic acid expression in response to dietary nitrogen levels in yak () and indigenous cattle () on the Qinghai-Tibetan plateau.

PubMed

Wang, H C; Shi, F Y; Hou, M J; Fu, X Y; Long, R J

2016-08-01

The gastrointestinal lumen can directly absorb all di- and tripeptide protein degradation products, and oligopeptide absorption depends on the specific peptide transport carriers, which are located in gastrointestinal epithelial cells on the brush border membrane. Yak () use N more efficiently than cattle do, which implies that yak have a specific mechanism of nonprotein utilization including a peptide absorption mechanism. However, this mechanism has not been clarified. Our objective was to explore whether yak possess any adaptive mechanisms of peptide absorption to survive in the harsh foraging environment of the Qinghai-Tibetan plateau. Twelve castrated males of each of 2 genotypes, yak () and indigenous cattle (), were fed diets of various N levels. The yak PepT1 (yPepT1) cDNA was cloned in omasum epithelial tissue. Our results showed that the full-length yPepT1 cDNA contains 2,805 bp, and a 2,121-bp open reading frame encodes a putative protein of 707 AA residues. The yPepT1 AA sequence identified 5 putative extracellular N-glycosylation sites (Asn, Asn, Asn, Asn, and Asn), 2 putative intracellular protein kinase A sites (Ser and Thr), and 3 intracellular putative protein kinase C sites (Ser, Ser, and Ser). The yPepT1 AA sequence was 99, 95, 86, and 83% identical to PepT1 from cattle (), sheep (), pigs (), and humans (), respectively. The relative PepT1 mRNA expression for indigenous cattle was greater than yak in the rumen, omasum, duodenum, ileum, and liver ( < 0.001); however, it was lower in jejunum tissue ( < 0.01). The relative PepT1 mRNA expression in response to increasing dietary N for both genotypes were linear in the rumen and jejunum ( < 0.10); quadratic or cubic in the reticulum ( < 0.01); linear or quadratic in the duodenum, ileum, and liver ( ≤ 0.01); and linear, quadratic, or cubic in the omasum ( < 0.001). Moreover, there were significant interactions between genotype and dietary N in rumen, reticulum, omasum, duodenum, jejunum, ileum, and liver tissues. In conclusion, the PepT1 profile and expression in gastrointestinal epithelial cells of yak varied from those of cattle, implying that yak have evolved a peptide transport mechanism to adapt the environment of the Qinghai-Tibetan plateau.

Epidemiological investigation and risk factors of Echinococcus granulosus in yaks (Bos grunniens), Tibetan pigs and Tibetans on Qinghai Tibetan plateau.

PubMed

Li, Kun; Zhang, Lihong; Zhang, Hui; Lei, Zhixin; Luo, Houqiang; Mehmood, Khalid; Shahzad, Muhammad; Lan, Yanfang; Wang, Meng; Li, Jiakui

2017-09-01

Echinococcus granulosus (E. granulosus) is a diverse zoonotic parasite and causes Cystic echinococcosis (CE) disease in humans and livestock. However, scare information is available about the epidemic situation of E. granulosus infection in yaks, Tibetan pigs and native Tibetans on the Qinghai Tibetan plateau. Therefore, a study was carried out to find prevalence and risk factors of E. granulosus in yaks, Tibetan pigs and Tibetans. Serum samples from yaks (1371), Tibetan pigs (454) and Tibetans (600) were collected and assessed by commercial ELISA kits. Multivariable logistic regression model was performed to find the variables possibly associated with exposure of E. granulosus infection in yaks, Tibetan pigs and Tibetan. The overall prevalence of E. granulosus in yaks was 6.49%. In different regions, the prevalence were ranged from 3.43% to 11.79%. In male and female yaks, the prevalence was 5.67% and 7.04%, respectively. In different ages, the prevalence were ranged from 2.20% to 10.9%. While, in different years, the prevalence was 3.61% in 2014, 9.66% in 2015, and 6.33% in 2016. According to the conditional stepwise logistic regression, three factors (region, age and year) were demonstrated to be risk factors influencing the prevalence of E. granulosus in yaks significantly (P<0.05). A total 33/454 of Tibetan pigs were positive for E. granulosus with the distribution of 5.47, 5.70 and 13.27% prevalence in Gongbo'gvamda, Mainling, and Nyingchi region, respectively. In male and female Tibetan pigs, the prevalence was 7.12% and 7.49% respectively, while region was considered as a significant (P<0.05) risk factor influencing the E. granulosus infection in Tibetan pigs. The total prevalence of E. granulosus infection in Tibetans was 1.83%, while in male and female Tibetans, the prevalence was 1.41% and 2.21%, respectively. In different ages, the prevalence were ranged from 0 to 3.21%. In Tibetans contacting animals or not was 2.41% and 0.54% respectively, and breeding dogs or not was 3.0% and 1.09%, respectively. Risk factors (gender age, contact animal and breed dog) were not significant (P>0.05). The present results reported the prevalence and associated risk factors of E. granulosus in yaks, Tibetan pigs and native Tibetans. These findings could have important epidemiological significance and a direct influence on the remote plateau. Copyright © 2017 Elsevier B.V. All rights reserved.

The complete sequence of mitochondrial genome of polled yak (Bos grunniens).

PubMed

Chu, Min; Wu, Xiaoyun; Liang, Chunnian; Pei, Jie; Ding, Xuezhi; Guo, Xian; Bao, Pengjia; Yan, Ping

2016-05-01

Generally speaking, the hornless trait is also known as polled. Although the POLL locus could be assigned to a 1.36-Mb interval in the centromeric region of BTA1 (Georges et al., 1993; Drögemüller et al., 2005)), and (Liu et al., 2014) reported a 147-kb segment that included three protein-coding genes was the most likely location of the POLL mutation in domestic yaks, the underlying genetic basis for the polled trait is still unknown. In this work, the complete mitochondrial genome sequence of polled yak was determined for the first time. The total length of the mitogenome is 16,324 bp long, with the base composition of 33.72% A, 27.25% T, 25.83% C, and 13.20% G. It contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 non-coding region (D-loop region). The gene order of polled yak mitogenome is identical to that observed in most other vertebrates. The complete mitogenome sequence information of polled yak will provide useful data for further studies on protection of genetic resources and phylogenetic relationships within Bos grunniens.

Genetic diversities of MT-ND1 and MT-ND2 genes are associated with high-altitude adaptation in yak.

PubMed

Shi, Yu; Hu, Yongsong; Wang, Jie; Elzo, Mauricio A; Yang, Xue; Lai, Songjia

2018-04-01

Tibetan yak (Bos grunniens) inhabiting the Qinghai-Tibet Plateau (QTP) where the average altitude is 4000 m, is specially adapted to live at these altitudes. Conversely, cattle (B. taurus) has been found to suffer from high-altitude hypertension or heart failure when exposed to these high altitudes. Two mitochondrial genes, MT-ND1 and MT-ND2, encode two subunits of NADH dehydrogenase play an essential role in the electron transport chain of oxidative phosphorylation (OXPHOS). We sequenced these two mitochondrial genes in two bovine groups (70 Tibetan yaks and 70 Xuanhan cattle) and downloaded 300 sequences of B. taurus (cattle), 93 sequences of B. grunniens (domestic yak), and 2 sequences of B. mutus (wild yak) from NCBI to increase our understanding of the mechanisms of adaptability to hypoxia at high altitudes in yaks compared to cattle. MT-ND1 SNP m.3907 C > T, present in all Tibetan yaks, was positively associated with high-altitude adaptation (p < .0006). Specially, mutation m.3638 A > G present in all cattle, resulting in the termination of transcription, was negatively associated with high-altitude adaptation (p < .0006). Additionally, MT-ND2 SNPs m.4351 G > A and m.5218 C > T also showed positive associations with high-altitude adaptation (p < .0004). MT-ND1 haplotypes H2, H3, H4, H6, and H7 showed positive associations but haplotype H20 had a negative association with high-altitude adaptation (p < .0008). Similarly, MT-ND2 haplotypes Ha1 Ha8, Ha10, and Ha11 were positively associated whereas haplotype Ha2 was negatively associated with adaptability to high-altitudes (p < .0008). Thus, MT-ND1 and MT-ND2 can be considered as candidate genes associated with adaptation to high-altitude environments.

Complete mitogenome sequencing and phylogenetic analysis of PaLi yak (Bos grunniens).

PubMed

Bao, Pengjia; Guo, Xian; Pei, Jie; Liang, Chunnian; Ding, Xuezhi; Min, Chu; Wang, Hongbo; Wu, Xiaoyun; Yan, Ping

2016-11-01

PaLi yak is a very important local breed in China; as a year-round grazing animal, it plays a very important role for the economic and native herdsmen. The PaLi yak complete mitochondrial DNA is sequenced in this study, the total length is 16,324 bp, containing 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a non-coding control region (D-loop region). The order and composition are similar to most of the other vertebrates. The base contents are: 33.72% A, 25.80% C, 13.21% G and 27.27% T; A + T (60.99%) was higher than G + C (39.01%). The phylogenetic relationships were analyzed using the complete mitogenome sequence, results showed that the genetic relationship between yak and cattle is distinct. These information provides useful data for further study on protection of genetic resources and the taxonomy of Bovinae.

Genome-wide Association Study Identifies Loci for the Polled Phenotype in Yak

PubMed Central

Wu, Xiaoyun; Wang, Kun; Ding, Xuezhi; Wang, Mingcheng; Chu, Min; Xie, Xiuyue; Qiu, Qiang; Yan, Ping

2016-01-01

The absence of horns, known as the polled phenotype, is an economically important trait in modern yak husbandry, but the genomic structure and genetic basis of this phenotype have yet to be discovered. Here, we conducted a genome-wide association study with a panel of 10 horned and 10 polled yaks using whole genome sequencing. We mapped the POLLED locus to a 200-kb interval, which comprises three protein-coding genes. Further characterization of the candidate region showed recent artificial selection signals resulting from the breeding process. We suggest that expressional variations rather than structural variations in protein probably contribute to the polled phenotype. Our results not only represent the first and important step in establishing the genomic structure of the polled region in yak, but also add to our understanding of the polled trait in bovid species. PMID:27389700

Lower Methane Emissions from Yak Compared with Cattle in Rusitec Fermenters

PubMed Central

Mi, Jiandui; Zhou, Jianwei; Huang, Xiaodan; Long, Ruijun

2017-01-01

Globally methane (CH4) emissions from ruminant livestock account for 29% of total CH4 emissions. Inherited variation about CH4 emissions of different animal species might provide new opportunity for manipulating CH4 production. Six rumen-simulating fermenters (Rusitec) were set up for this study lasting for 16 d. The diet consisted of forage to concentrate ratio of 50:50 with barley straw as the forage. Treated vessels were supplied with rumen fluid from yak or cattle (3 vessels per animal species). Microbial growth was measured using 15N as a marker. The microbial community structure from liquid- and solid-fraction of each vessel was determined based on the 16S rRNA genes targeting both bacteria and archaea with MiSeq platform. CH4 yield was lower when the inoculum used from yak than that from cattle (0.26 and 0.33 mmol CH4/g dry matter intake, respectively). Lower H2 production was observed in Rusitec fermenters with rumen fluid from yak compare with that from cattle (0.28 and 0.86 mmol/d, respectively). The apparent digestibility of neutral detergent fiber, the isovalerate percentage with respect to the total amount of volatile fatty acids, the hydrogen recovery, and the proportion of liquid-associated microbial nitrogen derived from ammonia-nitrogen were higher in Rusitec fermenters incubated with rumen fluid from cattle than that from yak. The relative abundances of methanogens were no difference between two animal species. We hypothesize that more H2 production contributes to the higher methane emissions in cattle compare with yak. PMID:28076447

Lower Methane Emissions from Yak Compared with Cattle in Rusitec Fermenters.

PubMed

Mi, Jiandui; Zhou, Jianwei; Huang, Xiaodan; Long, Ruijun

2017-01-01

Globally methane (CH4) emissions from ruminant livestock account for 29% of total CH4 emissions. Inherited variation about CH4 emissions of different animal species might provide new opportunity for manipulating CH4 production. Six rumen-simulating fermenters (Rusitec) were set up for this study lasting for 16 d. The diet consisted of forage to concentrate ratio of 50:50 with barley straw as the forage. Treated vessels were supplied with rumen fluid from yak or cattle (3 vessels per animal species). Microbial growth was measured using 15N as a marker. The microbial community structure from liquid- and solid-fraction of each vessel was determined based on the 16S rRNA genes targeting both bacteria and archaea with MiSeq platform. CH4 yield was lower when the inoculum used from yak than that from cattle (0.26 and 0.33 mmol CH4/g dry matter intake, respectively). Lower H2 production was observed in Rusitec fermenters with rumen fluid from yak compare with that from cattle (0.28 and 0.86 mmol/d, respectively). The apparent digestibility of neutral detergent fiber, the isovalerate percentage with respect to the total amount of volatile fatty acids, the hydrogen recovery, and the proportion of liquid-associated microbial nitrogen derived from ammonia-nitrogen were higher in Rusitec fermenters incubated with rumen fluid from cattle than that from yak. The relative abundances of methanogens were no difference between two animal species. We hypothesize that more H2 production contributes to the higher methane emissions in cattle compare with yak.

Genetic assessment of leech species from yak (Bos grunniens) in the tract of Northeast India.

PubMed

Chatterjee, Nilkantha; Dhar, Bishal; Bhattarcharya, Debasis; Deori, Sourabh; Doley, Juwar; Bam, Joken; Das, Pranab J; Bera, Asit K; Deb, Sitangshu M; Devi, Ningthoujam Neelima; Paul, Rajesh; Malvika, Sorokhaibam; Ghosh, Sankar Kumar

2018-01-01

Yak is an iconic symbol of Tibet and high altitudes of Northeast India. It is highly cherished for milk, meat, and skin. However, yaks suffer drastic change in milk production, weight loss, etc, when infested by parasites. Among them, infestation by leeches is a serious problem in the Himalayan belt of Northeast India. The parasite feeds on blood externally or from body orifices, like nasopharynx, oral, rectum, etc. But there has been limited data about the leech species infesting the yak in that region because of the difficulties in morphological identification due to plasticity of the body, changes in shape, and surface structure and thus, warrants for the molecular characterization of leech. In anticipation, this study would be influential in proper identification of leech species infesting yak track and also helpful in inventorying of leech species in Northeast India. Here, we investigated, through combined approach of molecular markers and morphological parameters for the identification of leech species infesting yak. The DNA sequences of COI barcode fragment, 18S and 28S rDNA, were analyzed for species identification. The generated sequences were subjected to similarity match in global database and analyzed further through Neighbour-Joining, K2P distance based as well as ML approach. Among the three markers, only COI was successful in delineating species whereas the 18S and 28S failed to delineate the species. Our study confirmed the presence of the species from genus Hirudinaria, Haemadipsa, Whitmania, and one species Myxobdella annandalae, which has not been previously reported from this region.

Isolation and characterization of a new enterovirus F in yak feces in the Qinghai-Tibetan Plateau.

PubMed

He, Huan; Tang, Cheng; Chen, Xinnuo; Yue, Hua; Ren, Yupeng; Liu, Yan; Zhang, Bin

2017-02-01

An enterovirus (EV) strain, designated as SWUN-AB001, was isolated in the Qinghai-Tibetan Plateau from a yak with severe diarrheal disease. The complete genome of strain SWUN-AB001 was 7,382 bp in length and shared 35.1-68.5% nt identities with bovine EVs belonging to a candidate new type EV-F7. Using the sequence difference values in the VP1 gene as a criterion for demarcating a new serotype/genotype in the Enterovirus genus, strain SWUN-AB001 had only a 71.1% nt and a 79.2% aa identity, in the VP1 region, with the most closely matched EV, further indicating that a new type of EV had been identified. Phylogenetic analysis of the nt sequence of the viral polyprotein and of VP1 genes demonstrated that the virus fell within the EV-F cluster, but was located in a unique lineage. Furthermore, a large-scale surveillance study indicated that the prevalence of this EV in yaks was 31.05% (95% CI = 25.5-37.6%) in 235 animals with diarrhea and 24.13% (95% CI = 17.4-32.4%) in 116 healthy yaks. However, there was no significant difference in virus prevalence between diarrheal and healthy samples. Interestingly, in the Tibet region, diarrheal feces had a higher incidence of EVs than feces of healthy yaks (odd ratios = 6.03, 95% CI = 1.93-18.86), indicating that the incidence of EV was potentially correlated with the clinical symptom of diarrhea in yaks.

Prevalence of antimicrobial resistance and integron gene cassettes in Escherichia coli isolated from yaks (Poephagus grunniens) in Aba Tibetan Autonomous Prefecture, China.

PubMed

Yang, Xin; Zou, Wencheng; Zeng, Jinxin; Xie, Shengze; An, Tianwu; Luo, Xiaolin; Chen, Danyu; Feng, Lan; Cheng, Guangyang; Cai, Run; Huang, Qianru; Wang, Hongning

2017-10-01

Escherichia coli (E. coli) is one of the most relevant opportunistic pathogenic bacteria as it may cause severe morbidity and mortality in yaks (poephagus grunniens). In recent years, several kinds of antibiotics have been widely used in Tibetan areas to treat the bacterial diseases, resulting in serious repercussions on the bacterial antibiotic resistance in yaks. This investigation was conducted in order to determine the prevalence of antimicrobial resistance and integron gene cassettes in E. coli isolated from yaks in Aba Tibetan Autonomous Prefecture (Aba TAP), China. A total of 278 non-duplicated fresh samples were collected from the yaks in Aba TAP for the isolation and identification of E. coli isolates. Antimicrobial susceptibility testing is performed by using the disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines (CLSI, 2013). Various antibiotic resistance genes and integron gene cassettes were detected by polymerase chain reaction (PCR) and sequencing. Overall, a total of 228 E. coli bacteria were isolated from the fresh faeces of yaks in four different geographical regions. 58% of those isolates showed multi-drug resistance capabilities (MDR) in our study. These isolated bacteria showed a high resistance rate to streptomycin (84%), cefotaxime (79%), amikacin (61%) and trimethoprim-sulfamethoxazole (54%). The most common antimicrobial resistance genes in the isolates were bla CTX-M , sul1, aph (3')-IIa, aac (3)-IIa, aac (6')-Ib, tetB, with respective detection rates of 65%, 46%, 35%, 13%, 11%, and 10%. Furthermore, 66% and 6% of the strains carried Class 1 and 2 integrons, respectively. However, the class 3 integron was not detected. Gene cassette arrays in the class 1 integron included aadA1, aadA7, aadA5, aadA17, dfrA1, dfrA5, dfrA1-aadA1, dfrA12-aadA2 and dfrA17-aadA5. The most prevalent gene cassette was aadA1 (20%). For the class 2 integron, dfrA1-sat2-aadA1 (6%) and dfrA1-sat1-aadA1 (0.4%) were also detected as part of this research. High multi-drug resistance rates have been discovered, as well as a prevalence of antibiotic resistance genes and integron gene cassettes in the E. coli isolated from the faeces of yak. This might create a potential problem for treatment of the yaks' bacterial infections as well as food hygiene for humans. It is therefore urgently necessary to begin continuous surveillance and analysis of antibiotic resistance and integron cassettes in other bacteria from yaks. Copyright © 2017 Elsevier Ltd. All rights reserved.

Wildfire smoke in the Siberian Arctic in summer: source characterization and plume evolution from airborne measurements

NASA Astrophysics Data System (ADS)

Paris, J.-D.; Stohl, A.; Nédélec, P.; Arshinov, M. Yu.; Panchenko, M. V.; Shmargunov, V. P.; Law, K. S.; Belan, B. D.; Ciais, P.

2009-09-01

We present airborne measurements of carbon dioxide (CO2), carbon monoxide (CO), ozone (O3), equivalent black carbon (EBC) and ultra fine particles over North-Eastern Siberia in July 2008 performed during the YAK-AEROSIB/POLARCAT experiment. During a "golden day" (11 July 2008) a number of biomass burning plumes were encountered with CO concentration enhancements of up to 500 ppb relative to a background of 90 ppb. Number concentrations of aerosols in the size range 3.5-200 nm peaked at 4000 cm-3 and the EBC content reached 1.4 μg m-3. These high concentrations were caused by forest fires in the vicinity of the landing airport in Yakutsk where during the descent measurements in fresh smoke could be made. We estimate a combustion efficiency of 90±3% based on CO and CO2 measurements. The emission factor of CO emitted was 59.6±15.2 g CO per kilogram of dry matter burned, suggesting an increase in the average northern hemispheric CO concentration of 3.0-7.2 ppb per million hectares of Siberian forest burned. For BC, we estimate an emission factor of 0.52±0.07 g BC kg-1, comparable to values reported in the literature. The emission ratio of ultra-fine particles (3.5-200 nm) was 26 cm-3 (ppb CO)-1, consistent with other airborne studies. The transport of identified biomass burning plumes was investigated using the FLEXPART Lagrangian model. Based on sampling of wildfire plumes from the same source but with different atmospheric ages derived from FLEXPART, we estimate that the e-folding lifetimes of EBC and ultra fine particles (between 3.5 and 200 nm in size) against removal and growth processes are 5.1 and 5.5 days, respectively, supporting lifetimes estimates used in various modelling studies.

Wildfire smoke in the Siberian Arctic in summer: source characterization and plume evolution from airborne measurements

NASA Astrophysics Data System (ADS)

Paris, J.-D.; Stohl, A.; Nédélec, P.; Arshinov, M. Yu.; Panchenko, M. V.; Shmargunov, V. P.; Law, K. S.; Belan, B. D.; Ciais, P.

2009-12-01

We present airborne measurements of carbon dioxide (CO2), carbon monoxide (CO), ozone (O3), equivalent black carbon (EBC) and ultra fine particles over North-Eastern Siberia in July 2008 performed during the YAK-AEROSIB/POLARCAT experiment. During a "golden day" (11 July 2008) a number of biomass burning plumes were encountered with CO mixing ratio enhancements of up to 500 ppb relative to a background of 90 ppb. Number concentrations of aerosols in the size range 3.5-200 nm peaked at 4000 cm-3 and the EBC content reached 1.4 μg m-3. These high concentrations were caused by forest fires in the vicinity of the landing airport in Yakutsk where measurements in fresh smoke could be made during the descent. We estimate a combustion efficiency of 90 ± 3% based on CO and CO2 measurements and a CO emission factor of 65.5 ± 10.8 g CO per kilogram of dry matter burned. This suggests a potential increase in the average northern hemispheric CO mixing ratio of 3.0-7.2 ppb per million hectares of Siberian forest burned. For BC, we estimate an emission factor of 0.52 ± 0.07 g BC kg-1, comparable to values reported in the literature. The emission ratio of ultra-fine particles (3.5-200 nm) was 26 cm-3 (ppb CO)-1, consistent with other airborne studies. The transport of identified biomass burning plumes was investigated using the FLEXPART Lagrangian model. Based on sampling of wildfire plumes from the same source but with different atmospheric ages derived from FLEXPART, we estimate that the e-folding lifetimes of EBC and ultra fine particles (between 3.5 and 200 nm in size) against removal and growth processes are 5.1 and 5.5 days respectively, supporting lifetime estimates used in various modelling studies.

Intraplate shearing and basin deformation in the Pacific Plate as a result of the Yakutat Block collision with North America

NASA Astrophysics Data System (ADS)

Reece, R.; Gulick, S. P.; Christeson, G. L.; Worthington, L. L.

2009-12-01

The Yakutat Block (YAK), an allochthonous terrane coupled to the Pacific Plate (PAC), collided with the North American plate ~10Ma and began subducting at the Aleutian Trench. Due to its thickness, the YAK is resistant to subduction compared to the PAC. As a result, the YAK is undergoing flat-slab subduction and now has developed its own vector relative to the PAC. High-resolution bathymetry data shows a 30km N-S trending ridge within the Surveyor Fan between the mouths of the Yakutat Sea Valley and Bering Trough. The ridge originates in the north at the base of the continental slope, which is coincident with the Transition Fault, the strike-slip boundary between the YAK and the PAC. The ridge exhibits greatest relief adjacent to the Transition Fault, and becomes less distinct farther from the shelf edge. As the highest relief feature in this part of the basin, the ridge has completely redefined sediment distribution patterns within the Surveyor Fan. Seismic reflection data reveal a sharp basement high beneath the ridge (1.1 sec of relief above “normal” basement in two-way travel time) as well as multiple strike-slip fault systems that are also N-S oriented. The ridge, basement high, and faults are aligned and co-located with an intraplate earthquake swarm on the PAC, which includes four events > 6.5 Mw that occurred from 1987-1992. This earthquake swarm is defined by mostly right-lateral strike-slip events, and is known as the Gulf of Alaska Shear Zone (GASZ). Based on the extent of seismicity, the GASZ extends 230km into the PAC. Tearing of oceanic crust on this scale is rare. A recent wide-angle seismic study shows the YAK to be a 20-25km thick mafic body while the 30 Myr old Pacific crust in the northern Gulf of Alaska is of normal thickness. Intraplate deformation occurring within the PAC could be the result of PAC-YAK coupling whereby YAK resistance to subduction is expressed as deformation in the thinner (weaker) PAC crust. Although a large tear in normal oceanic crust is unusual, preexisting zones of weakness within the PAC crust that are proximal to and under stress from the YAK may have proven to be a kinematically favorable localization for strain. These results support a recently proposed tectonic model wherein the differing YAK and PAC vectors caused the northern PAC to split into two different blocks, separated by the GASZ. In this model, the eastern block of the PAC would exhibit a counter-clockwise rotation that accounts for motion along the Transition Fault and GASZ. We will analyze this intraplate deformation zone using seismic imaging, bathymetry, and magnetic data in order to examine the cause of the strain localization and its southern termination, the influence of this shear zone on the sedimentary history, and relationship with the PAC-YAK interplate deformation along the Transition Fault.

Expression characteristics of BMP2, BMPR-IA and Noggin in different stages of hair follicle in yak skin.

PubMed

Song, Liang-Li; Cui, Yan; Yu, Si-Jiu; Liu, Peng-Gang; Liu, Jun; Yang, Xue; He, Jun-Feng; Zhang, Qian

2018-05-01

Bone morphogenetic protein 2 (BMP2), BMP receptor-IA (BMPR-IA), and the BMP2 antagonist Noggin are important proteins involved in regulating the hair follicle (HF) cycle in skin. In order to explore the expression profiles of BMP2, BMPR-IA, and Noggin in the HF cycle of yak skin, we collected adult yak skin in the telogen, proanagen, and midanagen phases of HFs and evaluated gene and protein expression by real-time quantitative polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry. qRT-PCR and western blotting results showed that BMP2 and BMPR-IA expression levels were highest in the telogen of HFs and higher than that of Noggin in the same phase. The expression of Noggin was significantly higher in proanagen and midanagen phases of HFs than in the telogen phase, with the highest expression observed in the proanagen phase. Moreover, the expression of Noggin in the proanagen phase was significantly higher than those of BMP2 and BMPR-IA during the same phase. Immunohistochemistry results showed that BMP2, BMPR-IA, and Noggin were expressed in the skin epidermis, sweat glands, sebaceous glands, HF outer root sheath, and hair matrix. In summary, the characteristic expression profiles of BMP2, BMPR-IA, and Noggin suggested that BMP2 and BMPR-IA had inhibitory effects on the growth of HFs in yaks, whereas Noggin promoted the growth of yak HFs, mainly by affecting skin epithelial cell activity. These results provide a basis for further studies of HF development and cycle transition in yak skin. Copyright © 2017. Published by Elsevier Inc.

Evaluation of composition and individual variability of rumen microbiota in yaks by 16S rRNA high-throughput sequencing technology.

PubMed

Guo, Wei; Li, Ying; Wang, Lizhi; Wang, Jiwen; Xu, Qin; Yan, Tianhai; Xue, Bai

2015-08-01

The Yak (Bos grunniens) is a unique species of ruminant animals that is important to agriculture of the Tibetan plateau, and has a complex intestinal microbial community. The objective of the present study was to characterize the composition and individual variability of microbiota in the rumen of yaks using 16S rRNA gene high-throughput sequencing technique. Rumen samples used in the present study were obtained from grazing adult male yaks (n = 6) in a commercial farm in Ganzi Autonomous Prefecture of Sichuan Province, China. Universal prokaryote primers were used to target the V4-V5 hypervariable region of 16S rRNA gene. A total of 7200 operational taxonomic units (OTUs) were obtained after sequence filtering and chimera removal. Within these OTUs, 0.56% belonged to Archaea (40 OTUs), 7.19% to unassigned species (518 OTUs), and the remaining OTUs (6642) in all samples were of bacterial origin. When examining the community structure of bacteria, we identified 23 phyla within 159 families after taxonomic summarization. Bacteroidetes and Firmicutes were the predominant phyla accounting for 39.68% (SD = 0.05) and 45.90% (SD = 0.06), respectively. Moreover, 3764 OTUs were identified as shared OTUs (i.e. represented in all yaks) and belonged to 35 genera, exhibiting highly variable abundance across individual samples. Phylogenetic placement of these genera across individual samples was examined. In addition, we evaluated the distance among the 6 rumen samples by adding taxon phylogeny using UniFrac, representing 24.1% of average distance. In summary, the current study reveals a shared rumen microbiome and phylogenetic lineage and presents novel information on composition and individual variability of the bacterial community in the rumen of yaks. Copyright © 2015. Published by Elsevier Ltd.

MicroRNA-200a regulates adipocyte differentiation in the domestic yak Bos grunniens.

PubMed

Zhang, Yongfeng; Wu, Xiaoyun; Liang, Chunnian; Bao, Pengjia; Ding, Xuezhi; Chu, Min; Jia, Congjun; Guo, Xian; Yan, Ping

2018-04-15

The domestic yak (Bos grunniens) is a culturally important animal that lives at high altitude and is farmed by Tibetan herders for its meat, milk, and other animal by-products. Within the animal, adipose tissue is an important store and source of energy and is used to maintain adequate body temperature during the extended cold seasons. Exploring the biomolecular role of microRNAs (miRNAs) in the regulation of growth, development, and metabolism of yak adipocytes may provide valuable insights into the physiology of adipogenesis in the yak. This study investigated whether and how miR-200a (a miRNA recently reported to promote adipogenesis in ST2 bone marrow stromal cells) regulates adipocyte differentiation in the yak. Expression levels of miR-200a gradually increased during day 0 to day 8 of adipocyte differentiation, and transfection of adipocytes with miR-200a enhanced lipid accumulation and triglyceride content compared to control (un-transfected) adipocytes. We additionally verified (using qRT-PCR analysis) that miR-200a increased the expression of adipocyte-specific genes involved in lipogenic transcription (PPARγ, ELVOL, and C/EBPα), fatty acid synthesis (ACC, ACS, SCD, and FAS), and fatty acid transport (DGAT, LPL, and FABP4). We also found that transfection of adipocytes with miR-200a resulted in suppression of the levels of noncanonical Wnt signaling transcription factors (Wnt5a, TAK1, and NLK). These results indicate that miRNA-200a plays an important role in promoting yak adipocyte differentiation that may operate via the suppression of noncanonical Wnt signaling. Copyright © 2018 Elsevier B.V. All rights reserved.

Isolation and characterization of spermatogenic cells from cattle, yak and cattleyak.

PubMed

Shah, Mujahid Ali; Xu, Chuanfei; Wu, Shixin; Zhao, Wangsheng; Luo, Hui; Yi, Chuanping; Liu, Wenjing; Cai, Xin

2018-06-01

Cattleyak forms the first generation in the cross-breeding of cattle (Bos taurus) and yak (Bos grunniens), the purpose of which is to increase the yak's performance in meat and milk production. The female cattleyak is fertile while the male remains sterile due to spermatogenic arrest. The spermatogenic cells (including spermatogonia and spermatocytes) of cattle, yak and cattleyak have not been successfully isolated so far. In this work, spermatogenic cells were isolated from these bovid species with the STA-PUT method that has been previously used for germ cell sorting in human and mouse, and the isolated cells could be used to investigate the mechanisms involved in male sterility observed in cattleyak. The characteristics and size of the isolated cells were investigated through microscopic examination, and the cell types were identified by RT-PCR amplification of the marker genes. The purity of spermatogonia and spermatocytes isolated from each bovid species was found to be higher than 85%. The spermatogonium diameter of cattle (10.10 ± 1.04 μm) and yak (14.90 ± 2.30 μm) were significantly larger (P < 0.01) than that of cattleyak (8.60 ± 0.92 μm). The spermatocyte diameter of cattle (19.40 ± 1.50 μm) and yak (20.50 ± 2.42 μm) were also significantly larger (P < 0.01) than that of cattleyak (17.70 ± 2.05 μm). Therefore, the STA-PUT was again validated to be a convenient, economical and efficient method for isolation of spermatogenic cells as it yields more cells within a short time frame. Copyright © 2018 Elsevier B.V. All rights reserved.

Effect of mitochondrial apoptotic activation through the mitochondrial membrane permeability transition pore on yak meat tenderness during postmortem aging.

PubMed

Wang, Lin-Lin; Han, Ling; Ma, Xiu-Li; Yu, Qun-Li; Zhao, Suo-Nan

2017-11-01

The effect of membrane permeability transition pore dependent mitochondrial apoptotic activation on yak meat tenderness was investigated. Results indicate that MPTP opening increased significantly and the mitochondrial membrane potential decreased markedly in the early aging process (P<0.05). Cytochrome c was released from the mitochondria to the cytoplasm via the MPTP in the early period. Meanwhile, the activation of procaspase-9 occurred earlier than that of procaspase-3. Cyclosporin A suppressed the MPTP opening, depolarization of the mitochondrial membrane potential, activities of caspase-9 and caspase-3, apoptosis rate, myofibril fragmentation index, reactive oxygen species generation, and Ca 2+ levels. These results demonstrated that MPTP mediated the release of cytochrome c in the mitochondrial apoptotic pathway. Furthermore, yak meat tenderness was improved by mitochondrial apoptotic pathway during aging. MPTP opening may be influenced by the ROS generation and Ca 2+ overloading in yak meat during postmortem aging. Copyright © 2017 Elsevier Ltd. All rights reserved.

Metabolomics Profiling of Serum and Urine in Three Beef Cattle Breeds Revealed Different Levels of Tolerance to Heat Stress.

PubMed

Liao, Yupeng; Hu, Rui; Wang, Zhisheng; Peng, Quanhui; Dong, Xianwen; Zhang, Xiangfei; Zou, Huawei; Pu, Qijian; Xue, Bai; Wang, Lizhi

2018-06-25

This study was to determine differences in the global metabolic profiles of serum and urine of Xuanhan yellow cattle, Simmental crossbred cattle (Simmental × Xuanhan yellow cattle), and cattle-yaks (Jersey × Maiwa yak) under heat stress (temperature-humidity index remained above 80 for 1 week). A total of 55 different metabolites associated with the three breeds were identified in the serum and urine samples by gas chromatography-mass spectrometry. The metabolic adaptations to heat stress are heterogeneous. Cattle-yaks mobilize a greater amount of body protein to release glucogenic amino acids to supply energy, whereas the tricarboxylic acid cycle is inhibited. Simmental crossbred cattle mobilize a greater amount of body fat to use free fatty acids as an energy source. In comparison with Simmental crossbred cattle and cattle-yaks, Xuanhan yellow cattle have higher glycolytic activity and possess a stronger antioxidant defense system and are, in conclusion, more adapted to hot and humid environments.

Effect of Dietary Types on Feed Intakes, Growth Performance and Economic Benefit in Tibetan sheep and Yaks on the Qinghai-Tibet Plateau during Cold Season

PubMed Central

Xu, Shixiao; Hu, Linyong; Zhao, Na; Liu, Zhe; Ma, Li; Liu, Hongjin; Zhao, Xinquan

2017-01-01

Pastoralists on the Tibetan alpine rangeland suffered great economic loss in cold season, due to serious live-weight loss of domestic livestock under traditional grazing management. This study aimed to evaluate the effect of dietary types (crude protein levels) on feed intakes, growth performance and economic returns of local Tibetan sheep and yaks during cold season. Twenty-four yearling Tibetan sheep (25.29±3.95 kg LW) and twenty two-year-old yaks (100.62±4.55 kg LW) with familiar body conditions were randomly assigned to four groups, fed oats hay (OH), oats silage (OS), total mixed ration (TMR) and traditionally grazed on the local cool-season pasture (TG), respectively, over a 135-day experiment. Daily dry matter intake was determined; all animals were weighed at the beginning and every 15 days of the 135-day experiment. Then, the total live-weight gain, average daily live-weight gain, gain rate, feed efficiency and net economic benefit were calculated. Results indicated that feed and nutrient intakes (DMI, DMI/kg LW, DMI/kg LW0.75 and CPI) of TMR, OH and OS were higher than TG (P < 0.05). Grazing animals suffered serious live-weight loss, while TMR, OS and OH significantly (P < 0.05) improved total live-weight gain and gain rate in both Tibetan sheep and yaks during the entire experiment. TMR worked better in animal performance and feed efficiency, obtained the highest breeding profit in both Tibetan sheep and yaks among four treatments (P < 0.05). When expressed on net economic benefit, TMR shared the highest net economic benefit in Tibetan sheep, OH shared the highest net economic benefit in yaks, but, no significant difference of net economic benefit in yaks fed TMR and OH diets was determined (P > 0.05). Results indicated that TMR was a reasonable diet in promoting feed intakes, animal performance, feed efficiency and economic returns in domestic livestock, which should be considered by local herdsmen to increase their breeding profit during cold season. PMID:28056054

Effect of Dietary Types on Feed Intakes, Growth Performance and Economic Benefit in Tibetan sheep and Yaks on the Qinghai-Tibet Plateau during Cold Season.

PubMed

Xu, Tianwei; Xu, Shixiao; Hu, Linyong; Zhao, Na; Liu, Zhe; Ma, Li; Liu, Hongjin; Zhao, Xinquan

2017-01-01

Pastoralists on the Tibetan alpine rangeland suffered great economic loss in cold season, due to serious live-weight loss of domestic livestock under traditional grazing management. This study aimed to evaluate the effect of dietary types (crude protein levels) on feed intakes, growth performance and economic returns of local Tibetan sheep and yaks during cold season. Twenty-four yearling Tibetan sheep (25.29±3.95 kg LW) and twenty two-year-old yaks (100.62±4.55 kg LW) with familiar body conditions were randomly assigned to four groups, fed oats hay (OH), oats silage (OS), total mixed ration (TMR) and traditionally grazed on the local cool-season pasture (TG), respectively, over a 135-day experiment. Daily dry matter intake was determined; all animals were weighed at the beginning and every 15 days of the 135-day experiment. Then, the total live-weight gain, average daily live-weight gain, gain rate, feed efficiency and net economic benefit were calculated. Results indicated that feed and nutrient intakes (DMI, DMI/kg LW, DMI/kg LW0.75 and CPI) of TMR, OH and OS were higher than TG (P < 0.05). Grazing animals suffered serious live-weight loss, while TMR, OS and OH significantly (P < 0.05) improved total live-weight gain and gain rate in both Tibetan sheep and yaks during the entire experiment. TMR worked better in animal performance and feed efficiency, obtained the highest breeding profit in both Tibetan sheep and yaks among four treatments (P < 0.05). When expressed on net economic benefit, TMR shared the highest net economic benefit in Tibetan sheep, OH shared the highest net economic benefit in yaks, but, no significant difference of net economic benefit in yaks fed TMR and OH diets was determined (P > 0.05). Results indicated that TMR was a reasonable diet in promoting feed intakes, animal performance, feed efficiency and economic returns in domestic livestock, which should be considered by local herdsmen to increase their breeding profit during cold season.

[Effect of altitude chronic hypoxia on liver enzymes and its correlation with ACE/ACE2 in yak and migrated cattle].

PubMed

Liu, Feng-yun; Hu, Lin; Li, Yu-xian; Liu, Shi-ming; Tang, Yong-ping; Qi, Sheng-gui; Yang, Lei; Wu, Tian-yi

2015-05-01

To investigate the difference of liver enzyme levels and its correlation with serum ACE/ACE2 among yak and cattle on Qinghai-Tibetan plateau, and to further explore the biochemical mechanism of their liver of altitude adaptation. The serum samples of yak were collected at 3,000 m, 3,500 m, 4,000 m and 4,300 m respectively, meanwhile the serum samples of migrated cattle on plateau (2,500 m) and lowland cattle (1,300 m) were also collected. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholinesterase (CHE), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP), serum lipase (LPS), angiotensin converting enzyme(ACE), angiotensin converting enzyme-2 (ACE2) in serum were measured by using fully automatic blood biochemcal analyzer. We analysed the differences of the above enzymes and its correlation with ACE/ACE2. We used one way analysis of variance (ANOVA). The levels of ALT in 4,000 m group and 4,300 m group of yak increased significantly compared with other groups, there were no statistically significant differences in AST, CHE, GGT, ACE/ACE2 levels of yaks at different altitudes. As compared to lowland cattle, the serum levels of AST and CHE were increased, the level of LPS and ACE was decreased significantly, respectively, and especially, the ratio of ACE/ACE2 of migranted cattle reduced nearly two times. The levels of LPS were significantly correlated to the ratio of ACE/ACE2 in yak (r = 0.357, P < 0.01), and a high correlation between ALP and ACE/ACE2 in lowland cattle( r = 0.418, P < 0.05), But the biggest contribution rate of the ratio of ACE/ACE2 was only 17.5% for the changes of the levels of liver enzyme. The results indicated that with the altitude increased did not significantly influence the changes of liver enzymes' activities in mountainous yaks but not in cattle. However, all above these changes weren't actually correlated to the ratio of ACE/ACE2.

Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes.

PubMed

Xiao, Xiao; Zi, Xiang-Dong; Niu, Hui-Ran; Xiong, Xian-Rong; Zhong, Jin-Cheng; Li, Jian; Wang, Li; Wang, Yong

2014-04-22

The competence for embryonic development after IVF is low in the yak, therefore, we investigated the effects of supplementation of FSH, LH and the proteasome inhibitor MG132 in IVM media on yak oocyte competence for development after IVF. In Experiment 1, yak cumulus-oocyte complexes (COCs) were in vitro matured (IVM) in TCM-199 with 20% fetal calf serum (FCS), 1 microg/mL estradiol-17beta, and different combinations of LH (50 or 100 IU/mL) and FSH (0, 1, 5, 10 microg/mL) at 38.6 degrees C, 5% CO2 in air for 24 h. Matured oocytes were exposed to frozen-thawed, heparin-capacitated yak sperm. Presumptive zygotes were cultured in SOF medium containing 6 mg/ml BSA, 0.5 mg/mL myoinositol, 3% (v/v) essential amino acids, 1% nonessential amino acids and 100 μg/mL L-glutamine (48 h, 38.5 degrees C, 5% CO2, 5% O2, and 90% N2). In Experiment 2, cumulus cells were collected at the end of IVM to determine FSHR and LHR mRNA expression by real-time PCR. In Experiment 3 and 4, COCs were cultured in the presence or absence of the proteasomal inhibitor MG132 from either 0-6 h or 18-24 h after initiation of maturation. The optimum concentration of FSH and LH in IVM media was 5 microg/mL FSH and 50 IU/mL LH which resulted in the greatest cleavage (79.1%) and blastocyst rates (16.1%). Both FSHR and LHR mRNA were detected in yak cumulus cells after IVM. Treatment with MG132 early in maturation reduced (P<0.05) cleavage and blastocyst rates. Conversely, treatment with MG132 late in maturation improved (P<0.05) blastocyst rate. Optimal results with MG132 were achieved at a concentration of 10 microM. An optimum concentration of FSH and LH in IVM medium, and treatment with MG132 late in maturation can improve yak oocytes competence for development after IVF.

Egypt’s Air War in Yemen

DTIC Science & Technology

2010-03-01

transports. 15 They also had Yak -11 piston-engine fighters, which were often used for reconnaissance, and Mi-4 transport helicopters in theater...happened with 20 Yak aircraft received from Czechoslovakia. Most remained on the airfields to rust, with some never leaving their crates. In the late

Reproduction in female yaks (Bos grunniens) and opportunities for improvement.

PubMed

Zi, Xiang-Dong

2003-03-01

This paper reviews seasonal breeding, puberty, postpartum anestrus, embryonic loss and calf survival and their constraints in female yaks. Methods for improving fertility in postpartum yak cows are also considered. Yaks are seasonal breeders with mating and conception restricted in the warm season. Puberty generally occurs in the 2nd to the 4th warm season following birth, i.e. between 13 and 36 months of age. The cows usually have a long postpartum anestrus period; only a small proportion of the cows return to estrus in the 1st breeding season after calving, most come into estrus in the 2nd and 3rd years. Nutritional status is the most important determinant of reproduction in female yaks. Reproductive success is a direct result of the availability of pasture determined by climate, season, and management practices. Milking delays puberty by reducing milk intake (restricted suckling) and growth rate for the calf. Milking interferes with grazing and prolongs the duration of postpartum acyclicity in cows. Calves born early in the season have a longer suckling season than those born later in the season before the onset of winter. Thus, they can have their first cycle in the breeding season of the following year, while those born late in the season may not have their first estrus until 25 or 26 months of age. Cows calving early in the season are more likely to return to estrus in the year of calving because they have a longer period to recover from the demand on body reserves before the onset of winter. Inbreeding in smallholder yak farms is also discussed and minimizing inbreeding by exchanging bulls among different herds is suggested. Reproductive efficiency can be improved by nutritional supplementation during the winter, however, the most cost-effective and practical strategy for this needs to be determined. Early weaning or restricted suckling may shorten the duration of postpartum acyclicity, however, it is impractical due to reduced growth rates and increased winter mortality of early weaned calves. A single treatment with either GnRH, or PGF(2alpha)+GnRH can successfully induce estrus in yak cows that calved in previous years (with or without calf) but did not calve in the current year, however, it has little effect in cows nursing a calf born in the current year. The effects of administration of exogenous progestogens plus GnRH on the fertility of yak cows are worthy of further study.

Key sources and seasonal dynamics of greenhouse gas fluxes from yak grazing systems on the Qinghai-Tibetan Plateau

NASA Astrophysics Data System (ADS)

Liu, Yang; Yan, Caiyu; Matthew, Cory; Wood, Brennon; Hou, Fujiang

2017-01-01

Greenhouse gas (GHG) emissions from livestock grazing systems are contributing to global warming. To examine the influence of yak grazing systems on GHG fluxes and relationships between GHG fluxes and environmental factors, we measured carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes over three key seasons in 2012 and 2013 from a range of potential sources, including: alpine meadows, dung patches, manure heaps and yak night pens, on the Qinghai-Tibetan Plateau. We also estimated the total annual global warming potential (GWP, CO2-equivalents) from family farm grazing yaks using our measured results and other published data. In this study, GHG fluxes per unit area from night pens and composting manure heaps were higher than from dung patches and alpine meadows. Increased moisture content and surface temperature of soil and manure were major factors increasing CO2 and CH4 fluxes. High contributions of CH4 and N2O (21.1% and 44.8%, respectively) to the annual total GWP budget (334.2 tonnes) strongly suggest these GHG other than CO2 should not be ignored when estimating GWP from the family farm grazing yaks on the Qinghai-Tibetan Plateau for the purposes of determining national and regional land use policies or compiling global GHG inventories.

Cloning of HSP90, expression and localization of HSP70/90 in different tissues including lactating/non-lactating yak (Bos grunniens) breast tissue.

PubMed

Liu, Penggang; Yu, Sijiu; Cui, Yan; He, Junfeng; Yu, Chuan; Wen, Zexing; Pan, Yangyang; Yang, Kun; Song, Liangli; Yang, Xue

2017-01-01

The aim of this study is to investigate the expression and localization of HSP70/90 in different tissues and explore the regulation effects of HSP70/90 at lactation period of female yaks. HSP90 mRNA was cloned from the heart samples of female yaks, Quantitative real-time (qRT-PCR), Western blotting (WB), immunohistochemistry and immunofluorescence assays were utilized to analyze the expressions of HSP70/90 mRNA and protein in different tissues. Sequence analysis showed that HSP90 is a conserved molecular chaperone of female yaks. The qRT-PCR, WB results showed that the expressions of HSP70/90 mRNA and protein were significantly different in different tissues, and 3-fold higher expression during the lactation period than the non-lactation period of breast tissue (P < 0.01). Immunohistochemistry and immunofluorescence assays results showed that HSP70/90 were located in the cardiac muscle cells, cerebellar medulla, theca cells lining at the reproductive system, and the mammary epithelia of the breasts. In addition, the expression level of HSP70 was higher than those of HSP90 in all examined tissues. Therefore, our results strongly suggest that the expression and localization of HSP70/90 could provide significant evidence to further research in tissue specific expression, and lactation function of female yaks.

Key sources and seasonal dynamics of greenhouse gas fluxes from yak grazing systems on the Qinghai-Tibetan Plateau

PubMed Central

Liu, Yang; Yan, Caiyu; Matthew, Cory; Wood, Brennon; Hou, Fujiang

2017-01-01

Greenhouse gas (GHG) emissions from livestock grazing systems are contributing to global warming. To examine the influence of yak grazing systems on GHG fluxes and relationships between GHG fluxes and environmental factors, we measured carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes over three key seasons in 2012 and 2013 from a range of potential sources, including: alpine meadows, dung patches, manure heaps and yak night pens, on the Qinghai-Tibetan Plateau. We also estimated the total annual global warming potential (GWP, CO2-equivalents) from family farm grazing yaks using our measured results and other published data. In this study, GHG fluxes per unit area from night pens and composting manure heaps were higher than from dung patches and alpine meadows. Increased moisture content and surface temperature of soil and manure were major factors increasing CO2 and CH4 fluxes. High contributions of CH4 and N2O (21.1% and 44.8%, respectively) to the annual total GWP budget (334.2 tonnes) strongly suggest these GHG other than CO2 should not be ignored when estimating GWP from the family farm grazing yaks on the Qinghai-Tibetan Plateau for the purposes of determining national and regional land use policies or compiling global GHG inventories. PMID:28106070

Key sources and seasonal dynamics of greenhouse gas fluxes from yak grazing systems on the Qinghai-Tibetan Plateau.

PubMed

Liu, Yang; Yan, Caiyu; Matthew, Cory; Wood, Brennon; Hou, Fujiang

2017-01-20

Greenhouse gas (GHG) emissions from livestock grazing systems are contributing to global warming. To examine the influence of yak grazing systems on GHG fluxes and relationships between GHG fluxes and environmental factors, we measured carbon dioxide (CO 2 ), methane (CH 4 ) and nitrous oxide (N 2 O) fluxes over three key seasons in 2012 and 2013 from a range of potential sources, including: alpine meadows, dung patches, manure heaps and yak night pens, on the Qinghai-Tibetan Plateau. We also estimated the total annual global warming potential (GWP, CO 2 -equivalents) from family farm grazing yaks using our measured results and other published data. In this study, GHG fluxes per unit area from night pens and composting manure heaps were higher than from dung patches and alpine meadows. Increased moisture content and surface temperature of soil and manure were major factors increasing CO 2 and CH 4 fluxes. High contributions of CH 4 and N 2 O (21.1% and 44.8%, respectively) to the annual total GWP budget (334.2 tonnes) strongly suggest these GHG other than CO 2 should not be ignored when estimating GWP from the family farm grazing yaks on the Qinghai-Tibetan Plateau for the purposes of determining national and regional land use policies or compiling global GHG inventories.

Observations on the Air War in Syria

DTIC Science & Technology

2013-04-01

comfortable with a trainer aircraft. In January 2012, the Syrian air force attempted to buy 40 Yak -130 trainers from Russia, but in July 2012...election-result-delay-coup-live. 32. “Russia Will Not Deliver Yak -130 Fighter Jets to Syria,” Airforce-technology.com, 9 July 2012, http://www.airforce

A model-based data analysis of the atmospheric methane above Siberia during YAK-AEROSIB airborne campaign in summer 2012

NASA Astrophysics Data System (ADS)

Arzoumanian, Emmanuel; Paris, Jean-Daniel; Pruvost, Arnaud; Berchet, Antoine; Pison, Isabelle; Arshinov, Mikhail; Belan, Boris

2014-05-01

High latitude regions are large sources of CH4 in the atmosphere, both natural from boreal wetlands and wildfires, and anthropogenic from natural gas extraction industry, especially in the Russian Arctic. Our current understanding of the extent and amplitude of the natural sources, as well as the large scale driving factors, remain highly uncertain (Kirschke et al., Nature Geosci., 2013). After a decade of pause, atmospheric methane seems to be increasing again, with a possible significant contribution from the wetlands of the northern high latitudes initiated by an unusual rise of regional temperatures in 2007 (Dlugokencky et al., 2009). This work aims at better understanding high latitude CH4 sources and sinks using atmospheric measurements and transport model. YAK-AEROSIB atmospheric airborne campaigns have been performed in order to provide observational data about the composition of Siberian air. In this work, we focus on the 2012 campaign which has been conducted on July 31st and August 1st. It consisted of five flights, performed in the troposphere from the boundary layer up to 8.5 km, connecting Novosibirsk to Yakutsk and back. This particular campaign was dominated by wildfires in Western and central Siberia. Therefore a chemistry-transport model (CHIMERE), combined with datasets for anthropogenic (EDGAR) emissions and models for wetlands (ORCHIDEE) and wildfire (GFED), has been used to interpret the collected data. From tagged tracers and model observation mismatch we describe results concerning CH4 fluxes in the Siberian territory. This work was funded by CNRS (France), the French Ministry of Foreign Affairs, CEA (France), Presidium of RAS (Program No. 4), Brunch of Geology, Geophysics and Mining Sciences of RAS (Program No. 5), Interdisciplinary integration projects of Siberian Branch of RAS (No. 35, No. 70, No. 131), Russian Foundation for Basic Research (grants No 14-05-00526, 14-05-00590). Kirschke, S., P. Bousquet, P. Ciais, M. Saunois, J.G. Canadell, E.J. Dlugokencky, P. Bergamaschi, D. Bergmann, D.R. Blake, L. Bruhwiler, P. Cameron-Smith, S. Castaldi, F. Chevallier, L. Feng, A. Fraser, M. Heimann, E.L. Hodson, S. Houweling, B. Josse, P.J. Fraser, P.B. Krummel, J.-F. Lamarque, R.L. Langenfelds, C. Le Quéré, V. Naik, S. O'Doherty, P.I. Palmer, I. Pison, D. Plummer, B. Poulter, R.G. Prinn, M. Rigby, B. Ringeval, M. Santini, M. Schmidt, D.T. Shindell, I.J. Simpson, R. Spahni, L.P. Steele, S.A. Strode, K. Sudo, S. Szopa, G.R. van der Werf, A. Voulgarakis, M. van Weele, R.F. Weiss, J.E. Williams, and G. Zeng, 2013: Three decades of global methane sources and sinks. Nature Geosci., 6, 813-823 Dlugokencky, E., J., and Bruhwiler, L. 2009. Observational constraints on recent increases in the atmospheric CH4 burden, Geophys. Res. Lett., 36 (18), 5p

A new tectonic model for southern Alaska

NASA Astrophysics Data System (ADS)

Reeder, J. W.

2013-12-01

S Alaska consists of a complex tectonic boundary that is gradational from subduction of Pacific Plate (PAC) beneath N American Plate (NA) in the W to a transform fault between these two plates in the SE. Adding complexity, the Yakutat Plate (YAK) is in between. The YAK is exposed in NE Gulf of Alaska and has been well mapped (Plafker, 1987). It is bound by the NA to the E at the Fairweather fault and by the PAC to the S. Relative to NA, YAK is moving 47 mm/yr N30°W and PAC is moving 51 mm/yr N20°W (Fletcher & Freymueller, 2003). The YAK and deeper PAC extend NW beneath the NA as flat slabs (Brocher et al., 1994). They subduct to the W and NW in Cook Inlet region (Ratchkovsky et al., 1997), resulting in the Cook Inlet volcanic arc. They also subduct farther NNW toward the Denali volcanic gap and fault. The subducted part of the YAK is split by a transform fault exposed at Montana Creek (MC) at 62°06'N to 62°10'N at 150°W. It extends S60°W toward the most N Cook Inlet volcano, Hayes, and extends N60°E beyond Talkeetna Mts. Right-lateral WSW motion and thick fault gauge have been documented by McGee (1978) on MC and a S60°W fault scarp cutting Quaternary deposits has been mapped (Reed & Nelson, 1980). Fuis et al. (2008) seismically recognized 110 km of missing YAP NW of Talkeetna Mts, which he thought was due to a 'tear' in the YAK to the far S. Nikoli Greenstone has been found in the Talkeetna Mts just S of this transform (Schmidt, 2003) that is 70 km SW of any other mapped Nikoli. This fault offset is also shown by 7.8 km/sec Vp depth contours, which represent the YAK (Eberhart-Phillips et al., 2006), as 110 km at N60°W. Based on magnetic data (Csejtey & Griscom, 1978; Saltus et al., 2007), the fault is regionally recognized as a 10× km zone on the WSW margin of the large S Alaska magnetic high. The fault zone has narrow WSW magnetic highs and depressions. This fault is also recognized on digital relief (Riehle et al., 1996); but, another pronounced N60°E linear feature also exists 20× km S, which trends into Mt. Spurr volcano. It could be another transform. If the MC transform is taking all the discrepancy between PAC and YAK, the S part of the fault would be moving relatively 9 mm/yr to S60°W. This transform has possibly been active for 12 million years. The Wrangell volcanoes with respect to YAK are associated with a spreading ridge. Yet, with respect to PAC, they are associated with a subduction zone (Stevens et al., 1984). The Totschunda and Fairweather faults are the new westward developing Denali transform. The Castle Mountain fault, located about 65 km to the SE of the MC transform, is oriented N65°E. It has had significant right-lateral offset of at least 30 km based on 7.8 km/sec Vp depth contours and of 26 km by magnetic offsets (Haeussler & Saltus, 2004). This older transform probably corresponds to Tertiary volcanics SW of the Mt Spurr/Hayes volcanic complex. Two active megathrust faults exist in south central Alaska; a 1964 type megathrust between PAC and YAK (Plafker, 1969), and a more continental megathrust between YAK and NA (Reeder, 2012). Based on Knik Arm subsidence events, these two types alternate and the next megathrust should occur in 350× years. This more continental megathrust would result in uplift of the N side of the Castle Mountain fault. It might even correspond to significant right-lateral movement on the seismically quiet MC transform.

Fatty acid composition of intramuscular fat from pastoral yak and Tibetan sheep

USDA-ARS?s Scientific Manuscript database

Fatty acid (FA) composition of intramuscular fat from mature male yak (n=6) and mature Tibetan sheep (n=6) grazed on the same pasture in the Qinghai-Tibetan Plateau was analyzed by gas chromatograph/mass spectrometer to characterize fat composition of these species and to evaluate possible differenc...

Paisang (Quercus griffithii): a keystone tree species in sustainable agroecosystem management and livelihoods in Arunachal Pradesh, India.

PubMed

Singh, Ranjay K; Singh, Anshuman; Garnett, Stephen T; Zander, Kerstin K; Lobsang; Tsering, Darge

2015-01-01

In a study of the traditional livelihoods of 12 Monpa and Brokpa villages in Arunachal Pradesh, India using social-ecological and participatory rural appraisal techniques, we found that the forest tree species paisang (Quercus griffithii, a species of oak) is vital to agroecosystem sustainability. Paisang trees are conserved both by individuals and through community governance, because their leaves play a crucial role in sustaining 11 traditional cropping systems of the Monpa peoples. An Indigenous institution, Chhopa, regulates access to paisang leaves, ensuring that the relationship between paisang and traditional field crop species within Monpa agroecosystems is sustainable. The Monpa farmers also exchange leaves and agricultural products for yak-based foods produced by the transhumant Brokpa, who are primarily yak herders. Yak herds also graze in paisang groves during winter. These practices have enabled the conservation of about 33 landraces, yak breeds, and a number of wild plants. Paisang thus emerged as a culturally important keystone species in the cultures and livelihoods of both Monpa and Brokpa. Ecological and conservation knowledge and ethics about paisang vary with gender, social systems, and altitudes. Labor shortages, however, have already caused some changes to the ways in which paisang leaves are used and yak grazing patterns are also changing in the face of changes in attitude among local landowners. Given new competing interests, incentives schemes are now needed to conserve the ecologically sustainable traditional livelihoods.

Paisang ( Quercus griffithii): A Keystone Tree Species in Sustainable Agroecosystem Management and Livelihoods in Arunachal Pradesh, India

NASA Astrophysics Data System (ADS)

Singh, Ranjay K.; Singh, Anshuman; Garnett, Stephen T.; Zander, Kerstin K.; Lobsang; Tsering, Darge

2015-01-01

In a study of the traditional livelihoods of 12 Monpa and Brokpa villages in Arunachal Pradesh, India using social-ecological and participatory rural appraisal techniques, we found that the forest tree species paisang ( Quercus griffithii, a species of oak) is vital to agroecosystem sustainability. Paisang trees are conserved both by individuals and through community governance, because their leaves play a crucial role in sustaining 11 traditional cropping systems of the Monpa peoples. An Indigenous institution, Chhopa, regulates access to paisang leaves, ensuring that the relationship between paisang and traditional field crop species within Monpa agroecosystems is sustainable. The Monpa farmers also exchange leaves and agricultural products for yak-based foods produced by the transhumant Brokpa, who are primarily yak herders. Yak herds also graze in paisang groves during winter. These practices have enabled the conservation of about 33 landraces, yak breeds, and a number of wild plants. Paisang thus emerged as a culturally important keystone species in the cultures and livelihoods of both Monpa and Brokpa. Ecological and conservation knowledge and ethics about paisang vary with gender, social systems, and altitudes. Labor shortages, however, have already caused some changes to the ways in which paisang leaves are used and yak grazing patterns are also changing in the face of changes in attitude among local landowners. Given new competing interests, incentives schemes are now needed to conserve the ecologically sustainable traditional livelihoods.

Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue

PubMed Central

Jiang, Ming Feng; Hu, Ming Jun; Ren, Hong Hui; Wang, Li

2015-01-01

Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp open reading frames, which encodes 148 amino acids (16.54 kDa) with a signal peptide of 18 amino acids, was sequenced. Further analysis indicated that the nucleic acid and amino acid sequences identities between yak and cow milk lysozyme were 89.04% and 80.41%, respectively. Recombinant yak milk lysozyme (rYML) was produced by Escherichia coli BL21 and Pichia pastoris X33. The highest lysozyme activity was detected for heterologous protein rYML5 (M = 1,864.24 U/mg, SD = 25.75) which was expressed in P. pastoris with expression vector pPICZαA and it clearly inhibited growth of Staphylococcus aureus. Result of the YML gene expression using quantitative polymerase chain reaction showed that the YML gene was up-regulated to maximum at 30 day postpartum, that is, comparatively high YML can be found in initial milk production. The phylogenetic tree indicated that the amino acid sequence was similar to cow kidney lysozyme, which implied that the YML may have diverged from a different ancestor gene such as cow mammary glands. In our study, we suggest that YML be a new c-type lysozyme expressed in yak mammary glands that plays a role as host immunity. PMID:26580446

Morphological study of the atrioventricular conduction system and Purkinje fibers in yak.

PubMed

Duan, Deyong; Yu, Sijiu; Cui, Yan; Li, Chaoxu

2017-07-01

We studied the morphology of the atrioventricular conduction system (AVCS) and Purkinje fibers of the yak. Light and transmission electron microscopy were used to study the histological features of AVCS. The distributional characteristics of the His-bundle, the left bundle branch (LBB), right bundle branch (RBB), and Purkinje fiber network of yak hearts were examined using gross dissection, ink injection, and ABS casting. The results showed that the atrioventricular node (AVN) of yak located in the right side of interatrial septum and had a flattened ovoid shape. The AVN of yak is composed of the slender, interweaving cells formed almost entirely of the transitional cells (T-cells). The His-bundle extended from the AVN, and split into left LBB and RBB at the crest of the interventricular septum. The LBB descended along the left side of interventricular septum. At approximately the upper 1/3 of the interventricular septum, the LBB typically divided into three branches. The RBB ran under the endocardium of the right side of interventricular septum, and extended to the base of septal papillary muscle, passed into the moderator band, crossed the right ventricular cavity to reach the base of anterior papillary muscle, and divided into four fascicles under the subendocardial layer. The Purkinje fibers in the ventricle formed a complex spatial network. The distributional and cellular component characteristics of the AVCS and Purkinje fibers ensured normal cardiac function. © 2017 Wiley Periodicals, Inc.

Sequence characterization of S100A8 gene reveals structural differences of protein and transcriptional factor binding sites in water buffalo and yak.

PubMed

Kathiravan, P; Goyal, S; Kataria, R S; Mishra, B P; Jayakumar, S; Joshi, B K

2011-01-01

The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analysis of coding DNA sequences (CDS) of S100A8 gene revealed 95% homology of buffalo sequence with cattle, 85% with pig and horse, 83% with dog, 72-73% with murines, and around 79% with primates and humans. Phylogenetic analysis of predicted CDS revealed distinct clustering of murines, primates, and domestic animals with bovines and bubalines forming a subcluster among farm animals. In silico translation of predicted CDS revealed a sequence of 89 amino acids with 7 amino acid changes between cattle and buffalo and 2 changes between cattle and yak. The search for Pfam family revealed the N-terminal calcium binding domain and the noncanonical EF hand domain in the carboxy terminus, with more variations being observed in the N-terminal domain among different species. Two amino acid changes observed in carboxy terminal EF hand domain resulted in altered secondary structure of yak S100A8 protein. Analysis of S100A8 gene promoter revealed 14 putative motifs for transcriptional factor binding sites. Two putative motifs viz. C/EBP and v-Myb were found to be absent in swamp buffalo as compared to river buffalo and cattle. Differences in the structure of S100A8 protein and the transcriptional factor binding sites identified in the present study need to be analyzed further for their functional significance in yak and swamp buffalo respectively. Copyright © Taylor & Francis Group, LLC

A profile of sphingolipids and related compounds tentatively identified in yak milk.

PubMed

Qu, S; Barrett-Wilt, G; Fonseca, L M; Rankin, S A

2016-07-01

This work characterized a fraction of constituents in yak milk within the realm of approximately 1,000 to 3,000 Da using matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. Eleven samples of yak milk powder from the Sichuan province of China were received by the Department of Food Science, University of Wisconsin-Madison, and stored at room temperature until analysis. Sample preparation involved delipidation and deproteinization of yak milk samples and cold ethanol precipitation. Subsequently, MALDI time-of-flight mass spectrometry was performed in positive ion, reflector mode (AB Sciex TOF/TOF 4800 MALDI; AB Sciex, Foster City, CA). The instrument was first calibrated with the manufacturer's 6-peptide mixture, and each spectrum was internally calibrated using the accurate mass of ACTH Fragment 18-39 standard peptide (protonated mass at m/z 2464.199) present in each sample. Laser power was adjusted for the calibration standards and for each sample so that the signal obtained for the most-abundant ion in each spectrum could be maximized, or kept below ~2×10(4) to preserve spectral quality. Structure and name based on mass were matched using the Metlin metabolite database (https://metlin.scripps.edu/index.php). Results of the current work for yak milk powder showed a large variety of sphingolipid structures with clusters around 1,200, 1,600, and 2,000 Da. The profiling matched several glycosphingolipids, such as gangliosides GA1, GD1a, GD1b, GD3, GM1, GM2, GM3, and GT2 and several other unique moieties, including deaminated neuraminic acid (KDN) oligosaccharides, and fucose containing gangliosides. Matrix preparation and MALDI time-of-flight parameters were important factors established in this work to allow high resolution profiling of complex sphingolipids in yak powder milk. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Technical note: Selection of suitable reference genes for studying gene expression in milk somatic cell of yak (Bos grunniens) during the lactation cycle.

PubMed

Bai, W L; Yin, R H; Zhao, S J; Jiang, W Q; Yin, R L; Ma, Z J; Wang, Z Y; Zhu, Y B; Luo, G B; Yang, R J; Zhao, Z H

2014-02-01

Quantitative real-time PCR is the most sensitive technique for gene expression analysis. Data normalization is essential to correct for potential errors incurred in all steps from RNA isolation to PCR amplification. The commonly accepted approach for normalization is the use of reference gene. Until now, no suitable reference genes have been available for data normalization of gene expression in milk somatic cells of lactating yaks across lactation. In the present study, we evaluated the transcriptional stability of 10 candidate reference genes in milk somatic cells of lactating yak, including ACTB, B2M, GAPDH, GTP, MRPL39, PPP1R11, RPS9, RPS15, UXT, and RN18S1. Four genes, RPS9, PPP1R11, UXT, and MRPL39, were identified as being the most stable genes in milk somatic cells of lactating yak. Using the combination of RPS9, PPP1R11, UXT, and MRPL39 as reference genes, we further assessed the relative expression of 4 genes of interest in milk somatic cells of yak across lactation, including ELF5, ABCG2, SREBF2, and DGAT1. Compared with expression in colostrum, the overall transcription levels of ELF5, ABCG2, and SREBF2 in milk were found to be significantly upregulated in early, peak, and late lactation, and significantly downregulated thereafter, before the dry period. A similar pattern was observed in the relative expression of DGAT1, but no significant difference was revealed in its expression in milk from late lactation compared with colostrum. Based on these results, we suggest that the geometric mean of RPS9, PPP1R11, UXT, and MRPL39 can be used for normalization of real-time PCR data in milk somatic cells of lactating yak, if similar experiments are performed. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Chronological Lifespan in Yeast Is Dependent on the Accumulation of Storage Carbohydrates Mediated by Yak1, Mck1 and Rim15 Kinases

PubMed Central

Tang, Yingzhi; Quan, Zhenzhen; Zhang, Zhe; Oliver, Stephen G.; Zhang, Nianshu

2016-01-01

Upon starvation for glucose or any other macronutrient, yeast cells exit from the mitotic cell cycle and acquire a set of characteristics that are specific to quiescent cells to ensure longevity. Little is known about the molecular determinants that orchestrate quiescence entry and lifespan extension. Using starvation-specific gene reporters, we screened a subset of the yeast deletion library representing the genes encoding ‘signaling’ proteins. Apart from the previously characterised Rim15, Mck1 and Yak1 kinases, the SNF1/AMPK complex, the cell wall integrity pathway and a number of cell cycle regulators were shown to be necessary for proper quiescence establishment and for extension of chronological lifespan (CLS), suggesting that entry into quiescence requires the integration of starvation signals transmitted via multiple signaling pathways. The CLS of these signaling mutants, and those of the single, double and triple mutants of RIM15, YAK1 and MCK1 correlates well with the amount of storage carbohydrates but poorly with transition-phase cell cycle status. Combined removal of the glycogen and trehalose biosynthetic genes, especially GSY2 and TPS1, nearly abolishes the accumulation of storage carbohydrates and severely reduces CLS. Concurrent overexpression of GSY2 and TSL1 or supplementation of trehalose to the growth medium ameliorates the severe CLS defects displayed by the signaling mutants (rim15Δyak1Δ or rim15Δmck1Δ). Furthermore, we reveal that the levels of intracellular reactive oxygen species are cooperatively controlled by Yak1, Rim15 and Mck1, and the three kinases mediate the TOR1-regulated accumulation of storage carbohydrates and CLS extension. Our data support the hypothesis that metabolic reprogramming to accumulate energy stores and the activation of anti-oxidant defence systems are coordinated by Yak1, Rim15 and Mck1 kinases to ensure quiescence entry and lifespan extension in yeast. PMID:27923067

Chronological Lifespan in Yeast Is Dependent on the Accumulation of Storage Carbohydrates Mediated by Yak1, Mck1 and Rim15 Kinases.

PubMed

Cao, Lu; Tang, Yingzhi; Quan, Zhenzhen; Zhang, Zhe; Oliver, Stephen G; Zhang, Nianshu

2016-12-01

Upon starvation for glucose or any other macronutrient, yeast cells exit from the mitotic cell cycle and acquire a set of characteristics that are specific to quiescent cells to ensure longevity. Little is known about the molecular determinants that orchestrate quiescence entry and lifespan extension. Using starvation-specific gene reporters, we screened a subset of the yeast deletion library representing the genes encoding 'signaling' proteins. Apart from the previously characterised Rim15, Mck1 and Yak1 kinases, the SNF1/AMPK complex, the cell wall integrity pathway and a number of cell cycle regulators were shown to be necessary for proper quiescence establishment and for extension of chronological lifespan (CLS), suggesting that entry into quiescence requires the integration of starvation signals transmitted via multiple signaling pathways. The CLS of these signaling mutants, and those of the single, double and triple mutants of RIM15, YAK1 and MCK1 correlates well with the amount of storage carbohydrates but poorly with transition-phase cell cycle status. Combined removal of the glycogen and trehalose biosynthetic genes, especially GSY2 and TPS1, nearly abolishes the accumulation of storage carbohydrates and severely reduces CLS. Concurrent overexpression of GSY2 and TSL1 or supplementation of trehalose to the growth medium ameliorates the severe CLS defects displayed by the signaling mutants (rim15Δyak1Δ or rim15Δmck1Δ). Furthermore, we reveal that the levels of intracellular reactive oxygen species are cooperatively controlled by Yak1, Rim15 and Mck1, and the three kinases mediate the TOR1-regulated accumulation of storage carbohydrates and CLS extension. Our data support the hypothesis that metabolic reprogramming to accumulate energy stores and the activation of anti-oxidant defence systems are coordinated by Yak1, Rim15 and Mck1 kinases to ensure quiescence entry and lifespan extension in yeast.

Adaptation strategies of yak to seasonally driven environmental temperatures in its natural habitat

NASA Astrophysics Data System (ADS)

Krishnan, G.; Paul, V.; Biswas, T. K.; Chouhan, V. S.; Das, P. J.; Sejian, V.

2018-05-01

The gradual increase of ambient temperature (TA) at high altitude can cause heat stress as an effect of climate change and may shift the traditional habitat of yak to further higher altitude. Therefore, an attempt has been made in this study to evaluate the thermo-adaptability of yaks to different seasons at high altitude. The adaptive capabilities of yaks were assessed based on different heat tolerance tests in relation to changes in rectal temperature (RT; °F), respiration rate (RR; breaths/min), pulse rate (PR; beats/min), and plasma heat shock protein (HSP) profile. The experiment was conducted in 24 yaks, divided into three groups based on age as calf (n = 8), adult (n = 8), and lactating cow (n = 8). Thermal adaptability was determined by temperature humidity index (THI), dairy search index (DSI), and Benezra's thermal comfort index (BTCI) along with HSP70 profile. The THI was higher (P < 0.01) in summer than winter which increased from lowest (40.87) to highest (61.03) in summer by 20 points, where yaks were under heat load beyond THI 52. The RT (100.09 ± 0.18 °F), RR (21.76 ± 0.18), and PR (59.78 ± 0.32) increased by 23-35%, and this was correlated to the higher values of DSI exceeding 1 in calves (1.35 ± 0.03), lactating cows (1.29 ± 0.04), and adults (1.23 ± 0.32) during summer in comparison to winter (0.98 ± 0.02). The BTCI also showed values greater (P < 0.01) than 2 in calves (3.47 ± 0.27), lactating cows (3.23 ± 0.28), and adults (2.98 ± 0.29) which reflected 49-75% increase in rectal temperature and respiration rate during summer. Further, heat stress was substantiated by threefold higher (P < 0.01) level of plasma HSP70 in calves (189.61 ± 3.90 pg/ml) followed by lactating cows (168.62 ± 3.03 pg/ml) and adults (155.33 ± 2.30 pg/ml) against the winter average of 87.92 ± 3.19 pg/ml. Present results revealed that yaks were experiencing heat stress in summer at an altitude of 3000 m above sea level and calves were more prone to heat stress followed by lactating cows and adults.

Mitochondrial genome data confirm that yaks can serve as the intermediate host of Echinococcus canadensis (G10) on the Tibetan Plateau.

PubMed

Wu, Yantao; Li, Li; Zhu, Guoqiang; Li, Wenhui; Zhang, Nianzhang; Li, Shuangnan; Yao, Gang; Tian, Wenjun; Fu, Baoquan; Yin, Hong; Zhu, Xingquan; Yan, Hongbin; Jia, Wanzhong

2018-03-09

Cervids used to be considered the only animal intermediate hosts of the G10 genotype of Echinococcus canadensis. Yaks are often herded in the Qinghai-Tibet Plateau, China, where echinococcosis remains prevalent. However, no E. canadensis G10 cases have been recorded in yaks until now. The aim of our study was to identify causative agents of echinococcosis in yaks in this region. Total genomic DNA was extracted from the germinal layer of one hydatid using a Blood and Tissue Kit. Full-length mitochondrial (mt) cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes were amplified by PCR. All purified PCR products were directly sequenced in both directions. Then seven pairs of overlap primers were designed to amplify the entire mt genome sequence of a suspected E. canadensis G10 isolate. Phylogenetic analyses were performed based on concatenated nucleotides from the 12 protein-coding genes of mt genomes of Echinococcus species in a Bayesian framework using MrBayes v3.1 and implementing the GTR + I + G model. Hydatids were found in yaks (n = 129) when organs were inspected at the slaughterhouse in Maqu county, Gannan Tibetan Autonomous Prefecture, Gansu Province, China in October 2016. Of these, 33 (25.6%) harbored up to a dozen hydatid cysts. One cyst from each yak was characterized by sequencing its mitochondrial (mt) cox1 and nad1 genes. On the basis of these sequence data, 32 cysts were identified as Echinococcus granulosus (sensu stricto) (G1-G3) and the remaining one was identified as the G10 genotype of E. canadensis. Its mt genome was then fully sequenced and compared with that of the G10 genotype in GenBank (AB745463). Phylogenetic analysis using complete mt genomes confirmed the Chinese cyst as belonging to the G10 genotype. To our knowledge, this is the first report globally of E. canadensis (G10) from yaks in China, which suggests that the G10 genotype has a wider geographical distribution and broader host range than previously believed. This genotype has therefore potential risks to human health and animal husbandry.

[Polymorphisms of inhibin α gene exon 1 in buffalo (Bubalus bubalis), gayal (Bos frontalis) and yak (Bos grunniens)].

PubMed

Miao, Yong-Wang; Ha, Fu; Gao, Hua-Shan; Yuan, Feng; Li, Da-Lin; Yuan, Yue-Yun

2012-08-01

To elucidate the genetic characteristics of the bovine Inhibin α subunit (INHA) gene, the polymorphisms in exon 1 of INHA and its bilateral sequences were assayed using PCR with direct sequencing in buffalo, gayal and yak. A comparative analysis was conducted by pooled the results in this study with the published data of INHA on some mammals including some bovine species together. A synonymous substitution c.73C>A was identified in exon 1 of INHA for buffalo, which results in identical encoding product in river and swamp buffalo. In gayal, two non-synonymous but same property substitutions in exon 1 of INHA, viz. c.62 C>T and c.187 G>A, were detected, which lead to p. P21L, p. V63M changes in INHA, respectively. In yak, nucleotide substitution c.62C> T, c.129A>G were found in exon 1 of INHA, the former still causes p. P21L substitution and the latter is synonymous. For the sequence of the 5'-flanking region of INHA examined, no SNPs were found within the species, but a substitution, c. -6T>G, was found. The nucleotide in this site in gayal, yak and cattle was c. -6G, whereas in buffalo it was c. -6T. Meanwhile, a 6-bp deletion, namely c. 262+31_262+36delTCTGAC, was found in the intron of buffalo INHA gene. For this deletion, wild types (+/+) account for main part in river buffalo while mutant types (-/-) are predominant in swamp buffalo. This deletion was not found in gayal, yak and cattle, though these all have another deletion in the intron of INHA, c. 262+78_262+79delTG. The results of sequence alignment showed that the substitutions c. 43A and c. 67G in exon 1 of INHA are specific to buffalo, whereas the substitutions c. 173A and c. 255G are exclusive to gayal, yak and cattle, and c. 24C, c. 47G, c. 174T and c. 206T are specific to goat. Furthermore, there are few differences among gayal, yak and cattle, but there relatively great differences between buffalo, goat and other bovine species regarding the sequences of INHA exon 1.

Developmental competence of mature yak vitrified-warmed oocytes is enhanced by IGF-I via modulation of CIRP during in vitro maturation.

PubMed

Pan, Yangyang; Cui, Yan; He, Honghong; Baloch, Abdul Rasheed; Fan, Jiangfeng; Xu, Gengquan; He, Junfeng; Yang, Kun; Li, Guyue; Yu, Sijiu

2015-12-01

The objective of this study was to investigate whether developmental competence of mature vitrified-warmed yak (Bos grunniens) oocytes can be enhanced by supplemented insulin-like growth factor I (IGF-1) during in vitro maturation (IVM), and its relationship with the expression of cold-inducible RNA-binding protein (CIRP). In experiment 1, immature yak oocytes were divided into four groups, and IVM supplemented with 0, 50, 100 and 200 ng/mL IGF-1 was evaluated; the mRNA and protein expression levels of CIRP in mature oocytes in the four groups were evaluated using quantitative real-time PCR and western blotting analyses. In experiment 2, the mature yak oocytes in the four groups were cryopreserved using the Cryotop (CT) method, followed by chemical activation and in vitro culture for two days and eight days to determine cleavage, blastocyst rates, and total cell number in the blastocysts. Mature yak oocytes without vitrification served as a control group. The outcomes were as following: (1) the expression of CIRP in the matured oocytes was up-regulated in the IGF-1 groups and was highest expression was observed in the 100 ng/mL IGF-1 treatment group. (2) In the vitrified-warmed groups, the rates of cleavage and blastocyst were also highest in the 100 ng/mL IGF-1 treatment group (81.04 ± 1.06%% and 32.16 ± 1.01%), which were close to the rates observed in groups without vitrification (83.25 ± 0.85% and 32.54 ± 0.34%). The rates of cleavage and blastocyst in the other vitrified-warmed groups were 70.92 ± 1.32% and 27.33 ± 1.31% (0 ng/mL); 72.73 ± 0.74% and 29.41 ± 0.84% (50 ng/mL); 72.43 ± 0.61% and 27.61 ± 0.59% (200 ng/mL), respectively. There was no significant difference in the total cell number per blastocysts between the vitrified-warmed groups and group without vitrification. Thus, we conclude that the enhancement in developmental competence of mature yak vitrified-warmed oocytes after the addition of IGF-1 during IVM might result from the regulation of CIRP expression in mature yak oocytes prior to vitrification. Copyright © 2015 Elsevier Inc. All rights reserved.

Should We Yak Back? Information Seeking among Yik Yak Users on a University Campus

ERIC Educational Resources Information Center

Price, Elizabeth

2018-01-01

Academic librarians have embraced tools such as e-mail or chat that allow them to deliver reference services virtually, but not many studies have advocated for using social networking sites (SNS) as a medium for answering user questions. Even as reference departments field fewer questions requiring in-depth resources or responses, librarians have…

Nonstationary phenomena in cosmic rays with E 0 ≤ 1018 eV according to the data of the Yakutsk EAS array

NASA Astrophysics Data System (ADS)

Glushkov, A. V.; Pravdin, M. I.

2012-07-01

The energy spectrum of cosmic rays and the fraction of muons with the threshold 1.0secθ GeV in the total number of charged particles in extensive air showers with energy E 0 ≥ 1017 eV according to Yakutsk array data collected during 35 years of its continuous operation in 1978-2012 have been analyzed. It has been shown that these characteristics are noticeably different in different time periods. Before 1996, the integral intensity of the spectrum at E 0 = 1017 eV varied near one stable position and then began to increase. It increased by (45 ± 5)% in seven years and, then, began to decrease. This phenomenon was accompanied a similar change in the fraction of muons and was caused by a significant increase in the average weight of the chemical composition of cosmic rays after 1996 as compared to preceding years.

Zebularine and scriptaid significantly improve epigenetic reprogramming of yak fibroblasts and cloning efficiency.

PubMed

Xiong, Xianrong; Lan, Daoliang; Li, Jian; Zhong, Jincheng; Zi, Xiangdong; Ma, Li; Wang, Yong

2013-08-01

Abnormal epigenetic reprogramming of the donor nucleus after somatic cell nuclear transfer (SCNT) is thought to be the main cause of low cloning efficiency. Following SCNT, the donor nucleus often fails to express early embryonic genes and establish a normal embryonic pattern of chromatin modification. Therefore, in this study, we have attempted to improve epigenetic reprogramming of the donor nucleus and cloned embryos with Zebularine and Scriptaid. Yak fibroblasts were treated with 20 μM Zebularine alone or 20 μM Zebularine plus 0.5 μM Scriptaid for 24 h, whereas yak cloned embryos were treated exclusively with 0.5 μM Scriptaid for 12 h. There was no effect on cellular viability and proliferation after drug treatment. The treatment of fibroblasts with Zebularine or Zebularine plus Scriptaid increased histone acetylation of histone 3 lysine 9 (H3K9), but decreased the level of DNA methylation of Oct-4 and Sox-2 promoter regions. When donor cells were used after Zebularine plus Scriptaid treatment to reconstruct cloned embryos and then treated with Scriptaid, the developmental competence and cryosurvival of embryos were improved significantly. In addition, the relative expression of Oct-4 and Sox-2 were increased significantly. The expression levels of Dnmt-1 and Hdac-1 were significantly decreased when fibroblasts and cloned embryos were treated with Zebularine or Scriptaid. This work provides functional evidence that treatment with Zebularine and Scriptaid modifies the epigenetic status of yak fibroblasts, subsequently enhancing in vitro developmental potential and the quality of yak cloned embryos.

Indoor air pollution from burning yak dung as a household fuel in Tibet

NASA Astrophysics Data System (ADS)

Xiao, Qingyang; Saikawa, Eri; Yokelson, Robert J.; Chen, Pengfei; Li, Chaoliu; Kang, Shichang

2015-02-01

Yak dung is widely used for cooking and heating in Tibet. We measured real-time concentrations of black carbon (BC) and fine particulate matter with an aerodynamic diameter of 2.5 μm or less (PM2.5) emitted by yak dung burning in six households with different living conditions and stove types in the Nam Co region, Tibet. We observed a much lower average BC/PM2.5 mass ratio (0.013, range 0.006-0.028) from dung combustion in this area than previously reported estimates, ranging between 0.05 and 0.11. Based on our measurements, estimated fuel use, and published emission factors of BC and PM2.5, about 0.4-1.7 Gg/year of BC is emitted by yak dung combustion in Tibet in addition to the previously estimated 0.70 Gg/year of BC for Tibetan residential sources. Our survey shows that most residents were aware of adverse health impacts of indoor yak dung combustion and approximately 2/3 of residents had already installed chimney stoves to mitigate indoor air pollution. However, our measurements reveal that, without adequate ventilation, installing a chimney may not ensure good indoor air quality. For instance, the 6-h average BC and PM2.5 concentrations in a stone house using a chimney stove were 24.5 and 873 μg/m3, respectively. We also observed a change in the BC/PM2.5 ratios before and after a snow event. The impact of dung moisture content on combustion efficiency and pollutant emissions needs further investigation.

The expression of epidermal growth factor (EGF) and its receptor (EGFR) during post-natal testes development in the yak.

PubMed

Pan, Y; Cui, Y; Yu, S; Zhang, Q; Fan, J; Abdul Rasheed, B; Yang, K

2014-12-01

Growth factors play critical role in cell proliferation, regulate tissue differentiation and modulate organogenesis. Several growth factors have been identified in the testes of various mammalian species in last few years. In present investigation, the objective was to determine the expression of epidermal growth factor (EGF) and the epidermal growth factor receptor (EGFR) in yak testicular tissue by relative quantitative real time polymerase chain reaction (RT-PCR), Western blot (WB) and immunohistochemistry (IHC) from mRNA and protein levels. The testicular tissues were collected from male yak at 6 and 24 months old. Results of RT-PCR and WB showed that the expression quantity of EGF and EGFR at 24 months of age was higher than at 6 months, and the increase rate of EGFR on mRNA and protein levels was higher than the increase rate EGF during post-natal testes development. Positive staining for EGF and EGFR was very low and mainly localized to Leydig cells testes at 6 months of age with immunohistochemistry, and seminiferous tubules were not observed. At 24 month of age, both the EGF and EGFR could be detected in Leydig cells, peritubular myoid cells, sertoli cells and germ cells of the yak testes. However, EGF and EGFR were localized to preferential adluminal compartment and basal compartment in the seminiferous tubules, respectively. In conclusion, the findings in present studies suggest that EGF and EGFR as important paracrine and/or autocrine regulators in yak testes development and spermatogenesis. © 2014 Blackwell Verlag GmbH.

Segmentation features and structural organization of the intrapulmonary artery of the yak.

PubMed

Zhou, Jinxing; Yu, Sijiu; He, Junfeng; Cui, Yan

2013-11-01

This study aims to systematically investigate intrapulmonary artery segmentation, blood vessel wall characteristics and structure organization, and the interrelation between intrapulmonary artery structure and plateau hypoxia adaptation in yak. The normal intrapulmonary artery structure of the yak had been studied using histological methods and transmission electron microscopy. The intrapulmonary artery of the yak was also examined using morphometric analysis and angiography. Results showed that the elastic intrapulmonary artery is divided into two types, namely, classical and transitional elastic segments. The muscular intrapulmonary artery is divided into three types, namely, transitional, classical muscular, and muscular arteriole segments. In the transitional elastic artery, elastic fibers and smooth muscles are linked through three models of ends, lateral branches, and branch tops. Two phenomena are possible for the transition from the elastic intrapulmonary artery to the muscular artery. One phenomenon postulates that a less elastic membrane is first increased and then suddenly decreased, and another supposes that the elastic membrane is gradually reduced and assembled in one to two layers before entering the transitional muscular artery. The smooth muscle of the intrapulmonary artery tunica media had more apophysis; it was physically connected with elastic membrane or fiber and composed of functionally resilient unit of the intrapulmonary arterial wall. Glycogenosomes increased in the muscular intrapulmonary artery smooth muscle cells. It exist one to two layers intact smooth muscle in intrapulmonary arteriole, the presence of intact smooth muscle in the intrapulmonary arteriole of the yak is a kind of structure adaptation to low-oxygen environment. Copyright © 2013 Wiley Periodicals, Inc.

The pulmonary circulation of some domestic animals at high altitude

NASA Astrophysics Data System (ADS)

Anand, I.; Heath, D.; Williams, D.; Deen, M.; Ferrari, R.; Bergel, D.; Harris, P.

1988-03-01

Pulmonary haemodynamics and the histology of the pulmonary vasculature have been studied at high altitude in the yak, in interbreeds between yaks and cattle, and in domestic goats and sheep indigenous to high altitudes together with crosses between them and low-altitude strains. Cattle at high altitude had a higher pulmonary arterial pressure than cattle at low altitude. The yak and two interbreeds with cattle (dzos and stols) had a low pulmonary arterial pressure compared with cattle, while the medial thickness of the small pulmonary arteries was less than would be expected in cattle, suggesting that the yak has a low capacity for hypoxic pulmonary vasoconstriction and that this characteristic is transmitted genetically. Goats and sheep showed haemodynamic evidence of a limited response of the pulmonary circulation to high altitude, but no evidence that the high altitude breeds had lost this response. There were no measurable differences in the thickness of the media of the small pulmonary arteries between high- and low-altitude breeds of goats and sheep. All these species showed prominent intimal protrusions of muscle into the pulmonary veins but no specific effect of high altitude in this respect.

Milk protein polymorphisms in cattle (Bos indicus), mithun (Bos frontalis) and yak (Bos grunniens) breeds and their hybrids indigenous to Bhutan.

PubMed

Dorji, Tashi; Namikawa, Takao; Mannen, Hideyuki; Kawamoto, Yoshi

2010-10-01

In the current study, milk protein variation was examined in cattle (Bos indicus), mithun (Bos frontalis), yak (Bos grunniens) and their hybrid populations in Bhutan to estimate genetic variability, conduct genetic characterization and assess the possibility of gene flow between mithun and cattle. Isoelectric focusing of 372 milk samples from 11 populations detected four molecular types of β-lactoglobulin (A, B, E and M), five molecular types of α(S1) -casein (A, B, C, E and X) and three molecular types of k-casein (A, B and X). Mithun and yak shared alleles but were found to exhibit different allele frequencies for the proteins studied. The degree of genetic variability within populations was measured by average heterozygosity and ranged from 24-40% in cattle, 26% for yak and 33% for mithun. We also resolved the traditional mithun and cattle hybridization system via principal component analysis. Our results suggested secondary introgression of mithun genes to the village Thrabum population, and a close genetic relationship between Bhutanese indigenous cattle and Indian cattle. © 2010 The Authors; Journal compilation © 2010 Japanese Society of Animal Science.

A large-scale serological survey of Akabane virus infection in cattle, yak, sheep and goats in China.

PubMed

Wang, Jidong; Blasdell, Kim R; Yin, Hong; Walker, Peter J

2017-08-01

Akabane virus (AKAV) is a member of the Simbu serogroup, classified in the genus Orthobunyavirus, family Bunyaviridae. AKAV infection can cause abortion, stillbirth, and congenital arthrogryposis and hydranencephaly in cattle and sheep. The distribution and prevalence of AKAV infection in China is still unknown. A total of 2731 sera collected from 2006 to 2015 in 24 provinces of China from cattle, sheep, goats and yak were examined by serum neutralisation test. The overall seroprevalence rates for AKAV antibodies were 21.3% in cattle (471/2215) and 12.0% (17/142) in sheep or goats, and 0% in yak (0/374). The results indicated widespread AKAV infection in China among cattle and sheep but yak appear to have a low risk of infection. Using a selection of 50 AKAV-positive and 25 AKAV-negative cattle sera, neutralisation tests were also conducted to detect antibodies to several other Simbu serogroup bunyaviruses and closely related Leanyer virus. Although inconclusive, the data suggest that both Aino virus and Peaton virus, which have been reported previously in Japan and Korea, may also be present in cattle in China. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

Adaptation strategies of yak to seasonally driven environmental temperatures in its natural habitat.

PubMed

Krishnan, G; Paul, V; Biswas, T K; Chouhan, V S; Das, P J; Sejian, V

2018-05-04

The gradual increase of ambient temperature (TA) at high altitude can cause heat stress as an effect of climate change and may shift the traditional habitat of yak to further higher altitude. Therefore, an attempt has been made in this study to evaluate the thermo-adaptability of yaks to different seasons at high altitude. The adaptive capabilities of yaks were assessed based on different heat tolerance tests in relation to changes in rectal temperature (RT; °F), respiration rate (RR; breaths/min), pulse rate (PR; beats/min), and plasma heat shock protein (HSP) profile. The experiment was conducted in 24 yaks, divided into three groups based on age as calf (n = 8), adult (n = 8), and lactating cow (n = 8). Thermal adaptability was determined by temperature humidity index (THI), dairy search index (DSI), and Benezra's thermal comfort index (BTCI) along with HSP70 profile. The THI was higher (P < 0.01) in summer than winter which increased from lowest (40.87) to highest (61.03) in summer by 20 points, where yaks were under heat load beyond THI 52. The RT (100.09 ± 0.18 °F), RR (21.76 ± 0.18), and PR (59.78 ± 0.32) increased by 23-35%, and this was correlated to the higher values of DSI exceeding 1 in calves (1.35 ± 0.03), lactating cows (1.29 ± 0.04), and adults (1.23 ± 0.32) during summer in comparison to winter (0.98 ± 0.02). The BTCI also showed values greater (P < 0.01) than 2 in calves (3.47 ± 0.27), lactating cows (3.23 ± 0.28), and adults (2.98 ± 0.29) which reflected 49-75% increase in rectal temperature and respiration rate during summer. Further, heat stress was substantiated by threefold higher (P < 0.01) level of plasma HSP70 in calves (189.61 ± 3.90 pg/ml) followed by lactating cows (168.62 ± 3.03 pg/ml) and adults (155.33 ± 2.30 pg/ml) against the winter average of 87.92 ± 3.19 pg/ml. Present results revealed that yaks were experiencing heat stress in summer at an altitude of 3000 m above sea level and calves were more prone to heat stress followed by lactating cows and adults.

Identification of candidate miRNAs and expression profile of yak oocytes before and after in vitro maturation by high-throughput sequencing.

PubMed

Xiong, X R; Lan, D L; Li, J; Zi, X D; Li, M Y

2016-12-01

Small RNA represents several unique non-coding RNA classes that have important function in a wide range of biological processes including development of germ cells and early embryonic, cell differentiation, cell proliferation and apoptosis in diverse organisms. However, little is known about their expression profiles and effects in yak oocytes maturation and early development. To investigate the function of small RNAs in the maturation process of yak oocyte and early development, two small RNA libraries of oocytes were constructed from germinal vesicle stage (GV) and maturation in vitro to metaphase II-arrested stage (M II) and then sequenced using small RNA high-throughput sequencing technology. A total of 9,742,592 and 12,168,523 clean reads were obtained from GV and M II oocytes, respectively. In total, 801 and 1,018 known miRNAs were acquired from GV and M II oocytes, and 75 miRNAs were found to be significantly differentially expressed: 47 miRNAs were upregulated and 28 miRNAs were downregulated in the M II oocytes compared to the GV stage. Among the upregulated miRNAs, miR-342 has the largest fold change (9.25-fold). Six highly expressed miRNAs (let-7i, miR-10b, miR-10c, miR-143, miR-146b and miR-148) were validated by real-time quantitative PCR (RT-qPCR) and consistent with the sequencing results. Furthermore, the expression patterns of two miRNAs and their potential targets were analysed in different developmental stages of oocytes and early embryos. This study provides the first miRNA profile in the mature process of yak oocyte. Seventy-five miRNAs are expressed differentially in GV and M II oocytes as well as among different development stages of early embryos, suggesting miRNAs involved in regulating oocyte maturation and early development of yak. These results showed specific miRNAs in yak oocytes had dynamic changes during meiosis. Further functional and mechanistic studies on the miRNAs during meiosis may beneficial to understanding the role of miRNAs on meiotic division. © 2016 Blackwell Verlag GmbH.

Insulinlike growth factor I improves yak (Bos grunniens) spermatozoa motility and the oocyte cleavage rate by modulating the expression of Bax and Bcl-2.

PubMed

Pan, Yangyang; Cui, Yan; Baloch, Abdul Rasheed; Fan, Jiangfeng; He, Junfeng; Li, Guyue; Zheng, Hongfei; Zhang, Yifu; Lv, Peng; Yu, Sijiu

2015-09-15

The aim of our present study was to examine the effects of insulinlike growth factor 1 (IGF-1) on yak sperm motility during in vitro capacitation and the relationship between the effects of IGF-1 on yak sperm motility and apoptosis was evaluated. Frozen-thawed yak spermatozoa were incubated at 38 °C for 1 hour in Tyrode's bicarbonate-buffered medium for sperm culture (Sp-TALP) with different concentrations (0, 50, 100, and 200 ng/mL) of IGF-1. In every treatment, the sperm motility was measured by a computer-assisted sperm analyzer system. The fertilizing ability of spermatozoa was evaluated on the basis of oocyte cleavage rate after insemination. The expression of Bax and Bcl-2 was examined by real-time polymerase chain reaction and Western blot for the messenger RNA and protein levels. It is interesting to note that IGF-1 improved yak spermatozoa motility and the cleavage rate of oocytes; these improvements were highest in the 100 ng/mL IGF-1 group, followed by the 200 ng/mL and 50 ng/mL groups, with the lowest improvements in motility and cleavage rates in groups without IGF-1. The expression level of Bax was downregulated by IGF-1, whereas Bcl-2 was upregulated. Both messenger RNA and Bax proteins were lowest in groups with 100 ng/mL IGF-1, where the Bcl-2 was the highest. Bax expression in the groups with IGF-1 was lower than that in the group without IGF-1, and Bcl-2 expression was higher in groups with IGF-1 than that in the group without IGF-1. In conclusion, this research reports that improvements in yak spermatozoa motility and the oocyte cleavage rate after the addition of IGF-I may be a result of the reduction of spermatozoa apoptosis rates by modulating the expression of Bax and Bcl-2. Copyright © 2015 Elsevier Inc. All rights reserved.

The response of gene expression associated with lipid metabolism, fat deposition and fatty acid profile in the longissimus dorsi muscle of Gannan yaks to different energy levels of diets

PubMed Central

Liu, Jianbin; Wu, Xiaoyun; Bao, Pengjia; Long, Ruijun; Guo, Xian; Ding, Xuezhi; Yan, Ping

2017-01-01

The energy available from the diet, which affects fat deposition in vivo, is a major factor in the expression of genes regulating fat deposition in the longissimus dorsi muscle. Providing high-energy diets to yaks might increase intramuscular fat deposition and fatty acid concentrations under a traditional grazing system in cold seasons. A total of fifteen adult castrated male yaks with an initial body weight 274.3 ± 3.14 kg were analyzed for intramuscular adipose deposition and fatty acid composition. The animals were divided into three groups and fed low-energy (LE: 5.5 MJ/kg), medium-energy (ME: 6.2 MJ/kg) and high-energy (HE: 6.9 MJ/kg) diets, respectively. All animals were fed ad libitum twice daily at 08:00–09:00 am and 17:00–18:00 pm and with free access to water for 74 days, including a 14-d period to adapt to the diets and the environment. Intramuscular fat (IMF) content, fatty acid profile and mRNA levels of genes involved in fatty acid synthesis were determined. The energy levels of the diets significantly (P<0.05) affected the content of IMF, total SFA, total MUFA and total PUFA. C16:0, C18:0 and C18:1n9c account for a large proportion of total fatty acids. Relative expression of acetyl-CoA carboxylase (ACACA), fatty acid synthase (FASN), stearoyl-CoA desaturase (SCD), sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ) and fatty acid-binding protein 4 (FABP4) was greater in HE than in LE yaks (P<0.05). Moreover, ME yaks had higher (P<0.05) mRNA expression levels of PPARγ, ACACA, FASN, SCD and FABP4 than did the LE yaks. The results demonstrate that the higher energy level of the diets increased IMF deposition and fatty acid content as well as increased intramuscular lipogenic gene expression during the experimental period. PMID:29121115

Pyrolysis of attapulgite clay blended with yak dung enhances pasture growth and soil health: Characterization and initial field trials.

PubMed

Rafiq, Muhammad Khalid; Joseph, Stephen D; Li, Fei; Bai, Yanfu; Shang, Zhanhuan; Rawal, Aditya; Hook, James M; Munroe, Paul R; Donne, Scott; Taherymoosavi, Sara; Mitchell, David R G; Pace, Ben; Mohammed, Mohanad; Horvat, Joseph; Marjo, Christopher E; Wagner, Avital; Wang, Yanlong; Ye, Jun; Long, Rui-Jun

2017-12-31

Recent studies have shown that the pyrolysis of biomass combined with clay can result in both lower cost and increase in plant yields. One of the major sources of nutrients for pasture growth, as well as fuel and building materials in Tibet is yak dung. This paper reports on the initial field testing in a pasture setting in Tibet using yak dung, biochar, and attapulgite clay/yak dung biochars produced at ratios of 10/90 and 50/50 clay to dung. We found that the treatment with attapulgite clay/yak dung (50/50) biochar resulted in the highest pasture yields and grass nutrition quality. We also measured the properties and yields of mixtures of clay/yak dung biochar used in the field trials produced at 400°C and 500°C to help determine a possible optimum final pyrolysis temperature and dung/clay ratio. It was observed that increasing clay content increased carbon stability, overall biochar yield, pore size, carboxyl and ketone/aldehyde functional groups, hematite and ferrous/ferric sulphate/thiosulphate concentration, surface area and magnetic moment. Decreasing clay content resulted in higher pH, CEC, N content and an enhanced ability to accept and donate electrons. The resulting properties were a complex function of both processing temperature and the percentage of clay for the biochars processed at both 400°C and 500°C. It is possible that the increase in yield and nutrient uptake in the field trial is related to the higher concentration of C/O functional groups, higher surface area and pore volume and higher content of Fe/O/S nanoparticles of multiple oxidation state in the 50/50 clay/dung. These properties have been found to significantly increase the abundance of beneficial microorganisms and hence improve the nutrient cycling and availability in soil. Further field trials are required to determine the optimum pyrolysis production conditions and application rate on the abundance of beneficial microorganisms, yields and nutrient quality. Copyright © 2017 Elsevier B.V. All rights reserved.

Isolation, identification and fibrolytic characteristics of rumen fungi grown with indigenous methanogen from yaks (Bos grunniens) grazing on the Qinghai-Tibetan Plateau.

PubMed

Wei, Y-Q; Yang, H-J; Luan, Y; Long, R-J; Wu, Y-J; Wang, Z-Y

2016-03-01

To obtain co-cultures of anaerobic fungi and their indigenously associated methanogens from the rumen of yaks grazing on the Qinghai-Tibetan Plateau and investigate their morphology features and ability to degrade lignocellulose. Twenty fungus-methanogen co-cultures were obtained by Hungate roll-tube technique. The fungi were identified as Orpinomyces, Neocallimastix and Piromyces genera based on the morphological characteristics and internal transcribed spacer 1 sequences analysis. All methanogens were identified as Methanobrevibacter sp. by 16S rRNA gene sequencing. There were four types of co-cultures: Neocallimastix with Methanobrevibacter ruminantium, Orpinomyces with M. ruminantium, Orpinomyces with Methanobrevibacter millerae and Piromyces with M. ruminantium among 20 co-cultures. In vitro studies with wheat straw as substrate showed that the Neocallimastix with M. ruminantium co-cultures and Piromyces with M. ruminantium co-cultures exhibited higher xylanase, filter paper cellulase (FPase), ferulic acid esterase, acetyl esterase activities, in vitro dry matter digestibility, gas, CH4 , acetate production, ferulic acid and p-coumaric acid releases. The Neocallimastix frontalis Yak16 with M. ruminantium co-culture presented the strongest lignocellulose degradation ability among 20 co-cultures. Twenty fungus-methanogen co-cultures were obtained from the rumen of grazing yaks. The N. frontalis with M. ruminantium co-cultures were highly effective combination for developing a fermentative system that bioconverts lignocellulose to high activity fibre-degrading enzyme, CH4 and acetate. The N. frontalis with M. ruminantium co-cultures from yaks grazing on the Qinghai-Tibetan Plateau present great potential in lignocellulose biodegradation industry. © 2015 The Society for Applied Microbiology.

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in 1-2-month-old highland yaks in Qinghai Province, China.

PubMed

Wang, Geping; Wang, Guanghua; Li, Xiuping; Zhang, Xueyong; Karanis, Gabriele; Jian, Yingna; Ma, Liqing; Karanis, Panagiotis

2018-06-01

Cryptosporidium and Giardia are ubiquitous parasites that infect humans and animals. Few reports are available on the prevalence of these two protozoan parasites in yaks (Bos grunniens). In this study, 344 faecal samples were collected from yaks with diarrhoea in the Chenduo and Nangqian counties of Qinghai Province, China. Cryptosporidium spp. and Giardia duodenalis were detected by light and immunofluorescence microscopy and nested PCR (nPCR). Fifteen samples were positive (4.5%) by Kinyoun staining, 40 (11.6%) samples were positive by immunofluorescence test (IFT), and 39 (11.3%) samples were positive by nPCR for Cryptosporidium spp., Cryptosporidium bovis (11/39, 28.2%) was the most prevalent species, followed by C. ryanae (6/39, 15.4%), C. andersoni (5/39, 12.8%), C. struthionis (5/39, 12.8%), C. parvum (5/39, 12.8%), C. hominis (4/39, 10.3%) and C. canis (3/39, 7.7%). Thirteen out of 344 (3.8%) samples were positive for Giardia by simple microscopy, 20 (5.8%) by IFT and 18 samples (5.2%) yak faecal samples were Giardia positive by nPCR. Two G. duodenalis assemblages (B, E) were detected in this study. Nine positive samples for G. duodenalis assemblage E were from the towns of Xiewu (8/9, 4.9%) and Xiangda (1/9, 1.3%), and nine positive samples (9/9, 8.5%) for G. duodenalis assemblage B were from the town of Zhenqin. This report provides information about infection with Cryptosporidium species and G. duodenalis assemblages in domesticated 1-2-month-old highland yaks living in the Qinghai-Tibet Plateau region of China.

Complete mitochondrial genome of the a rare subspecies of genus Bos, Tianzhu white yak from Tibetan area in China.

PubMed

E, Guangxin; Na, Ri-Su; Zhao, Yong-Ju; Gao, Hui-Jiang; An, Tian-Wu; Huang, Yong-Fu

2016-01-01

The population of domestic yak, Tianzhu white yak, from Tibetan area in China is considered as a rare Bos grunniens species. We first determined and annotated its complete mitochondrial genome. The mitogenome is 16,319 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and a control region. As in other mammals, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes, which are encoded on the light strand. Its overall base composition is A: 33.7%, T: 27.2%, C: 25.8% and G: 13.2%. The complete mitogenome of the new subspecies of Bos grunniens could provide an important data to further explore the taxonomic status of the subspecies.

Does Yik Yak Promote Risky Health Behavior on College Campuses?

PubMed

Wombacher, Kevin; Reno, Jenna E; Williams, Gregory A; Johnson, Lauren

2018-04-01

This study offers an analysis of how healthy and risky behaviors are discussed on an emerging social media platform. We sought to understand what behaviors are communicated and if they are encouraged or discouraged. We completed a content analysis of messages (n = 3,776) posted to Yik Yak captured three times a day on 4 days of the week across two separate weeks. We analyzed messages to determine the category of the behavior, an appraisal of the behavior, and normative voting feedback. Our results show that risky behaviors were discussed with greater frequency and received more user votes than healthy behaviors. Exposure to these messages could influence other students' perceptions of norms associated with these risky behaviors. We suggest further research to determine how Yik Yak may affect normative perceptions on college campuses.

Effects of grazing and precipitation on herbage biomass, herbage nutritive value, and yak performance in an alpine meadow on the Qinghai-Tibetan Plateau.

PubMed

Miao, Fuhong; Guo, Zhenggang; Xue, Ran; Wang, Xianzhi; Shen, Yuying

2015-01-01

The Qinghai-Tibetan Plateau is a very large land unit and an important terrestrial ecosystem within the Eurasian continent. Because of the harsh climate associated with the high altitude, alpine meadows on the plateau are susceptible to degradation from overgrazing. For this region, and for other alpine meadow pastures internationally, there is a need to define the sustainable stocking rate, to develop sound policy pertaining to future land use. Here we report biomass and liveweight gain per animal and per ha for pastures grazed by yaks at high, medium, or low stocking rates over 4 growing seasons from 2010 to 2013. Measures of herbage nutritive value are reported. The influence of inter-year variation in precipitation on standing herbage biomass was also evaluated. Higher precipitation increased standing herbage biomass and herbage nutritive value, indicating that vegetation suffered summer water deficit even in this environment. The sustainable stocking rate in this environment was determined to be approximately 1 yak ha-1 (grown from 80 kg to 120 kg liveweight in 90 d). At this stocking rate, yak weight gain per ha was 88% of that achieved at higher stocking rates typically used by farmers, but with little or no evidence of land degradation.

Effects of Grazing and Precipitation on Herbage Biomass, Herbage Nutritive Value, and Yak Performance in an Alpine Meadow on the Qinghai–Tibetan Plateau

PubMed Central

Miao, Fuhong; Guo, Zhenggang; Xue, Ran; Wang, Xianzhi; Shen, Yuying

2015-01-01

The Qinghai–Tibetan Plateau is a very large land unit and an important terrestrial ecosystem within the Eurasian continent. Because of the harsh climate associated with the high altitude, alpine meadows on the plateau are susceptible to degradation from overgrazing. For this region, and for other alpine meadow pastures internationally, there is a need to define the sustainable stocking rate, to develop sound policy pertaining to future land use. Here we report biomass and liveweight gain per animal and per ha for pastures grazed by yaks at high, medium, or low stocking rates over 4 growing seasons from 2010 to 2013. Measures of herbage nutritive value are reported. The influence of inter-year variation in precipitation on standing herbage biomass was also evaluated. Higher precipitation increased standing herbage biomass and herbage nutritive value, indicating that vegetation suffered summer water deficit even in this environment. The sustainable stocking rate in this environment was determined to be approximately 1 yak ha-1 (grown from 80 kg to 120 kg liveweight in 90 d). At this stocking rate, yak weight gain per ha was 88% of that achieved at higher stocking rates typically used by farmers, but with little or no evidence of land degradation. PMID:26039322

Molecular characterization and epigenetic regulation of Mei1 in cattle and cattle-yak.

PubMed

Li, Bojiang; Wu, Wangjun; Luo, Hua; Liu, Zequn; Liu, Honglin; Li, Qifa; Pan, Zengxiang

2015-11-15

Mei1 is required for the homologous recombination of meiosis during the mammalian spermatogenesis. However, the knowledge about bovine Mei1 (bMei1) is still limited. In the present study, we cloned and characterized the bMei1, and investigated the epigenetic regulatory mechanism of bMei1 expression in vivo and in vitro. The full length coding region of bMei1 was 3819bp, which encoded a polypeptide of 1272 amino acids. Real-time PCR showed that the mRNA expression level of bMei1 in the testis of cattle-yak with meiotic arrest and male infertility was significantly decreased as compared with cattle (P<0.01). Conversely, the methylation levels of bMei1 promoter and gene body in the testis of cattle-yak were significantly increased. Additionally, the expression level of bMei1 in bovine mammary epithelial cells (BMECs) was activated by treatment with the methyltransferase inhibitor 5-aza-2' deoxycytidine (5-Aza-CdR). Our data suggest that bMei1 may play an important role in the meiosis of spermatogenesis and may be involved in cattle-yak male sterility, and its transcription was regulated by DNA methylation. Copyright © 2015 Elsevier B.V. All rights reserved.

Importance of functional ingredients in yak milk-derived food on health of Tibetan nomads living under high-altitude stress: a review.

PubMed

Guo, Xusheng; Long, Ruijun; Kreuzer, Michael; Ding, Luming; Shang, Zhanhuan; Zhang, Ying; Yang, Yang; Cui, Guangxin

2014-01-01

Tibetan nomads have lived since ancient times in the unique and harsh environment of the Qinghai-Tibetan Plateau with average altitudes over 4000 m. These people have been able to live and multiply healthily over numerous generations under the extreme stress of high-altitude environment, including cold, hypoxia, and strong ultraviolet radiation, and with a simple diet devoid of vegetables and fruits for most of the year. Their survival depends heavily on yak milk, and its products comprise the main portion of their daily diet. In this review, yak milk and its derived products are examined in detail and compared with milk from other ruminant species. Yak milk products seem to be particularly rich in functional and bioactive components, which may play a role in maintaining the health status of Tibetan nomads. This includes particular profiles of amino acids and fatty acids, and high levels of antioxidant vitamins, specific enzymes, and bacteria with probiotic activity (yoghurt is the main food). Based on that, it is proposed that the Tibetan nomads have developed a nutritional mechanism adapted to cope with the specific challenges posed by the environment of the world's highest plateau. Systematic studies are required to demonstrate this in a more mechanistic way.

Molecular detection and characterization of Theileria infection in cattle and yaks from Tibet Plateau Region, China.

PubMed

Qin, Gege; Li, Youquan; Liu, Junlong; Liu, Zhijie; Yang, Jifei; Zhang, Lin; Liu, Guangyuan; Guan, Guiquan; Luo, Jianxun; Yin, Hong

2016-07-01

Theileriosis continues to threaten the livestock industry worldwide, but comprehensive epidemiological surveys for this disease have not been conducted in the Tibet Plateau Region, China. In this study, we screened 154 cattle blood samples from the Tibet Plateau Region (Lhasa, Lhoka, and Tianzhu), China, for detection of Theileria pathogens by polymerase chain reaction (PCR) with species-specific primers. The results revealed that the prevalence was 6.9 % (2/29) for Theileria orientalis and 27.6 % (8/29) for Theileria sinensis in Lhasa, 0 % (0/30) for T. orientalis and 26.7 % (8/30) for T. sinensis in Lhoka, and 0 % (0/95) for T. orientalis and 30.5 % (29/95) for T. sinensis in Tianzhu. Interestingly, Theileria luwenshuni, which was a previously reported pathogenic Theileria sp. in sheep and goats, was detected in blood samples from cattle and yaks for the first time, with a prevalence of 10 % (3/30) in Lhoka and 1.1 % (1/95) in Tianzhu. No other Theileria sp. was detected in these samples. T. sinensis and T. orientalis infections were detected in cattle and yaks, and T. luwenshuni was discovered for the first time in cattle and yaks in the Tibet Plateau Region, China.

Ground and aircraft-based methane measurements in Siberia: source attribution using tracers and models

NASA Astrophysics Data System (ADS)

Arzoumanian, E.; Paris, J. D.; Pruvost, A.; Peng, S.; Turquety, S.; Berchet, A.; Pison, I.; Helle, J.; Arshinov, M.; Belan, B. D.

2015-12-01

Methane (CH4) is the second most important anthropogenic greenhouse gas. It is also naturally emitted by a number of processes, including microbial activity in wetlands, permafrost degradation and wildfires. Our current understanding of the extent and amplitude of its natural sources, as well as the large scale driving factors, remain highly uncertain (Kirschke et al., Nature Geosci., 2013). Furthermore, high latitude regions are large natural sources of CH4 in the atmosphere. Observing boreal/Arctic CH4 variability and understanding its main driving processes using atmospheric measurements and transport model is the task of this work. YAK-AEROSIB atmospheric airborne campaigns (flights in the tropospheric layer up to 9 km connecting the two cities of Novosibirsk and Yakutsk) and continuous measurements at Fonovaya Observatory (60 km west of Tomsk - 56° 25'07"N, 84° 04'27"E) have been performed in order to provide observational data on the composition of Siberian air. The study is focused on 2012, during which a strong heat wave impacted Siberia, leading to the highest mean daily temperature values on record since the beginning of the 20th century. This abnormal drought has led to numerous large forest fires. A chemistry-transport model (CHIMERE), combined with datasets for anthropogenic (EDGAR) emissions and models for wetlands (ORCHIDEE) and wildfires (APIFLAME), is used to determine contributions of CH4 sources in the region. Recent results concerning CH4 fluxes and its atmospheric variability in the Siberian territory derived from a modeled-based analysis will be shown and discussed. This work was funded by CNRS (France), the French Ministry of Foreign Affairs, CEA (France), Presidium of RAS (Program No. 4), Brunch of Geology, Geophysics and Mining Sciences of RAS (Program No. 5), Interdisciplinary integration projects of Siberian Branch of RAS (No. 35, No. 70, No. 131), Russian Foundation for Basic Research (grants No 14-05-00526, 14-05-00590). Kirschke, S. et al. : Three decades of global methane sources and sinks. Nature Geosci., 6, 813-823

[The risk factors and dangerous regimens of alcohol consumption in physicians' community].

PubMed

Savvina, I V; Grigor'ev, G I; Tulasynova, N Iu

2014-01-01

The study was carried out to determine percentage of hazardous, dangerous and possibly dependent alcohol consumption among physicians of Yakutsk. The relationship between social hygienic aspects accompanying hazardous and dangerous alcohol consumption was analyzed. The risk factors were established. The means of prevention of alcohol dependence among physicians were proposed.

The effect of space weather on human heart diseases in subauroral latitudes

NASA Astrophysics Data System (ADS)

Samsonov, S. N.; Kleimenova, N. G.; Kozyreva, O. V.; Petrova, P. G.

2014-12-01

In this work the relationship between emergency medical calls for myocardial infarction in Yakutsk (subauroral geomagnetic latitudes) and parameters of the space weather near maximum (1992) and minimum (1998) geomagnetic activity is studied. The comparison of the seasonal behavior of the number of calls with the simultaneous seasonal behavior of deaths from myocardial infarctions at low latitudes (Bulgaria) exhibited significant differences. Namely, in Bulgaria, the maximum and minimum of infarctions were observed in winter and in summer, respectively; in Yakutsk, several observed maximums coincided with the sharp and considerable increase in planetary geomagnetic activity. An analysis of experimental results made it possible to suppose that, in subauroral latitudes, unlike low latitudes, a major role in the increase in the number of infarctions is played by the increase in geomagnetic activity, namely, by the appearance of night magnetospheric substorms, which are also observed in subauroral latitudes in magnetically disturbed times. Substorms are always accompanied by irregular geomagnetic Pi1 pulsations with periods of 0.5-3 Hz. These pulsations can be biotropic, like stable quasi-sinusoidal geomagnetic Pc1 pulsations in middle and low latitudes.

Effects of high pressure treatment and temperature on lipid oxidation and fatty acid composition of yak (Poephagus grunniens) body fat.

PubMed

Wang, Qiang; Zhao, Xin; Ren, Yanrong; Fan, Enguo; Chang, Haijun; Wu, Hongbin

2013-08-01

Effects of high-pressure treatment (100 MPa to 600 MPa) on lipid oxidation and composition of fatty acids in yak body fat at 4 °C and 15 °C were investigated for up to 20 days storage. 400 and 600 MPa treatments increase the level of thiobarbituric acid-reactive substances (TBARS) 335% and 400% (p<0.05), respectively. Composition analysis shows that 600 MPa treatment induces a lower (p<0.05) percentage of polyunsaturated fatty acids, and C22:6 decreased significantly. A significant decrease in PUFA/SFA and n-6/n-3 PUFA values was observed at the end of storage. Samples treated at the lower pressures gave good sensory acceptability. It is concluded that a higher-pressure treatment is important in catalyzing lipid oxidation and the evolution of fatty acids in pressure-treated yak body fat. Copyright © 2013 Elsevier Ltd. All rights reserved.

Study of Ultra-High Energy Cosmic Rays from Extensive Air Showers Radio Emission

NASA Astrophysics Data System (ADS)

Petrov, Igor; Kozlov, Vladimir; Petrov, Zim; Knurenko, Stanislav; Pravdin, Mikhail

The study of cosmic rays with the help of radio detection from extensive air showers may be an alternative to traditional detecting methods, which use a large area array installed with hundreds and thousands of scintillation detectors for charged particles, or the detectors of measuring the emission produced by relativistic particles of EAS in the optical wavelengths. Processes that lead to the emission of electromagnetic radiation are well known and calculations show that the air shower radio emission depends on the processes of development of the electromagnetic cascade, i.e. related with the longitudinal development of the shower, with the magnetic field near sea level etc. In this regard, there is a question to establish the correlation between characteristics of EAS both longitudinal and lateral development and radio emission parameters observed when air shower particles pass through the atmosphere. For this purpose, in Yakutsk, radio array for detecting air shower radio emission was established. The array consists of the antenna field on which crossed antennas are installed; antennas oriented E - W and N - S. Radio emission measurements are conducted at frequency 32 MHz, free from industrial noise. In 2008 - 2013 years, Yakutsk array has measured several seasons of registration of EAS events, including showers with energies above 10 (19) eV. In the course of the data analysis the following results were obtained: a) lateral distribution of the radio signal plotted as a function of distance from the shower axis ; b) a correlation between the amplitude of the radio signal with the energy of the shower, which is determined by measuring the fluxes of charged particles , muons and EAS Cerenkov radiation (energy balance method); c) we made evaluation of the depth of maximum development of the shower using form of radio emission LDF measured in ultra-high energy showers; g) a comparison of the Yakutsk array data with data from other arrays.

Molecular characterization of Cryptosporidium from animal sources in Qinghai province of China.

PubMed

Karanis, Panagiotis; Plutzer, Judit; Halim, Norhaliza Abdul; Igori, Khatanbaatar; Nagasawa, Hideyuki; Ongerth, Jerry; Liqing, Ma

2007-11-01

The presence of Cryptosporidium oocysts in 20 zoo animals of the Xining Zoo, 16 farm yaks and 42 farm goats in Qinghai province, China was investigated by an immunofluorescence test (IFT). The species and/or genotypes were determined by nested polymerase chain reaction (PCR) and sequence analysis of a fragment of the small subunit (SSU) rRNA gene. Cryptosporidium oocysts were found in 16 zoo animals, 2 yaks, and 15 goats by IFT. The IFT positive samples were further investigated by PCR, and 16 of them were found to be positive by that method also. Sequence analysis of the PCR products derived from Cryptosporidium oocysts from Black leopard (Panthera pardus), Heijing He (Grus nigricollis), Barbary sheep (Ammotragus lervia), Takin (Budorcas taxicolor), Lesser panda (Ailurus fulgens), and White-eared pheasant (Crossoptilon crossoptilon) fecal samples matched that of Cryptosporidium parvum mouse genotype. Sequence analyses of other PCR products were consistent with cervine genotype Cryptosporidium from Ibex (Capra ibex), a novel Cryptosporidium genotype from a wild yak (Bos mutus), C. bovis-like genotype from one goat sample and also a novel Cryptosporidium genotype from one other separate goat sample. The present work reports the first data on Cryptosporidium infections in animals from the Qinghai province of mountainous central western China and the first findings of the 'cervine' genotype in Capra ibex, C. bovis-like genotype and the new Cryptosporidium spp. in farm goat and in wild yak.

Experimental mouse lethality of Escherichia coli strains isolated from free ranging Tibetan yaks.

PubMed

Rehman, Mujeeb Ur; Zhang, Hui; Wang, Yajing; Mehmood, Khalid; Huang, Shucheng; Iqbal, Muhammad Kashif; Li, Jiakui

2017-08-01

The present study has examined the virulence potential of Escherichia coli isolates harboring at least one virulence gene (associated with ExPEC or InPEC pathotype and belonging to different phylogenetic groups: A, B1, B2 or D), isolated from free ranging Tibetan yak feces. The E. coli isolates (n = 87) were characterized for different serogroups and a mouse model of subcutaneous-infection was used to envisage the virulence within these E. coli strains. Of the 87 E. coli isolates examined, 23% of the E. coli isolates caused lethal infections in a mouse model of subcutaneous infection and were classified as killer. Moreover, the majority of the killer strains belonged to phylogroup A (65%) and serogroup O 60 or O 101 (35%). Phylogroup B1, serogroups O 60 and O 101 were statistically associated with the killer status (P < 0.05). However, positive associations (OR >1) were observed between the killer status isolates and all other bacterial virulence traits. This study comprises the first report on the virulence potential of E. coli strains isolated from free-ranging Tibetan yaks feces. Our findings suggest that pathogenic E. coli of free ranging yaks is highly worrisome, as these feces are used as manures by farmers and therewith pose a health risk to humans upon exposure. Copyright © 2017 Elsevier Ltd. All rights reserved.

Dynamic changes of yak (Bos grunniens) gut microbiota during growth revealed by polymerase chain reaction-denaturing gradient gel electrophoresis and metagenomics

PubMed Central

Nie, Yuanyang; Zhou, Zhiwei; Guan, Jiuqiang; Xia, Baixue; Luo, Xiaolin; Yang, Yang; Fu, Yu; Sun, Qun

2017-01-01

Objective To understand the dynamic structure, function, and influence on nutrient metabolism in hosts, it was crucial to assess the genetic potential of gut microbial community in yaks of different ages. Methods The denaturing gradient gel electrophoresis (DGGE) profiles and Illumina-based metagenomic sequencing on colon contents of 15 semi-domestic yaks were investigated. Unweighted pairwise grouping method with mathematical averages (UPGMA) clustering and principal component analysis (PCA) were used to analyze the DGGE fingerprint. The Illumina sequences were assembled, predicted to genes and functionally annotated, and then classified by querying protein sequences of the genes against the Kyoto encyclopedia of genes and genomes (KEGG) database. Results Metagenomic sequencing showed that more than 85% of ribosomal RNA (rRNA) gene sequences belonged to the phylum Firmicutes and Bacteroidetes, indicating that the family Ruminococcaceae (46.5%), Rikenellaceae (11.3%), Lachnospiraceae (10.0%), and Bacteroidaceae (6.3%) were dominant gut microbes. Over 50% of non-rRNA gene sequences represented the metabolic pathways of amino acids (14.4%), proteins (12.3%), sugars (11.9%), nucleotides (6.8%), lipids (1.7%), xenobiotics (1.4%), coenzymes, and vitamins (3.6%). Gene functional classification showed that most of enzyme-coding genes were related to cellulose digestion and amino acids metabolic pathways. Conclusion Yaks’ age had a substantial effect on gut microbial composition. Comparative metagenomics of gut microbiota in 0.5-, 1.5-, and 2.5-year-old yaks revealed that the abundance of the class Clostridia, Bacteroidia, and Lentisphaeria, as well as the phylum Firmicutes, Bacteroidetes, Lentisphaerae, Tenericutes, and Cyanobacteria, varied more greatly during yaks’ growth, especially in young animals (0.5 and 1.5 years old). Gut microbes, including Bacteroides, Clostridium, and Lentisphaeria, make a contribution to the energy metabolism and synthesis of amino acid, which are essential to the normal growth of yaks. PMID:28183172

On Robust Key Agreement Based on Public Key Authentication

NASA Astrophysics Data System (ADS)

Hao, Feng

We describe two new attacks on the HMQV protocol. The first attack raises a serious question on the basic definition of "authentication" in HMQV, while the second attack is generally applicable to many other protocols. In addition, we present a new authenticated key agreement protocol called YAK. Our approach is to depend on well-established techniques such as Schnorr's signature. Among all the related protocols, YAK appears to be the simplest so far. We believe simplicity is an important engineering principle.

Comparative analysis of the microRNA transcriptome between yak and cattle provides insight into high-altitude adaptation.

PubMed

Guan, Jiuqiang; Long, Keren; Ma, Jideng; Zhang, Jinwei; He, Dafang; Jin, Long; Tang, Qianzi; Jiang, Anan; Wang, Xun; Hu, Yaodong; Tian, Shilin; Jiang, Zhi; Li, Mingzhou; Luo, Xiaolin

2017-01-01

Extensive and in-depth investigations of high-altitude adaptation have been carried out at the level of morphology, anatomy, physiology and genomics, but few investigations focused on the roles of microRNA (miRNA) in high-altitude adaptation. We examined the differences in the miRNA transcriptomes of two representative hypoxia-sensitive tissues (heart and lung) between yak and cattle, two closely related species that live in high and low altitudes, respectively. In this study, we identified a total of 808 mature miRNAs, which corresponded to 715 pre-miRNAs in the two species. The further analysis revealed that both tissues showed relatively high correlation coefficient between yak and cattle, but a greater differentiation was present in lung than heart between the two species. In addition, miRNAs with significantly differentiated patterns of expression in two tissues exhibited co-operation effect in high altitude adaptation based on miRNA family and cluster. Functional analysis revealed that differentially expressed miRNAs were enriched in hypoxia-related pathways, such as the HIF-1α signaling pathway, the insulin signaling pathway, the PI3K-Akt signaling pathway, nucleotide excision repair, cell cycle, apoptosis and fatty acid metabolism, which indicated the important roles of miRNAs in high altitude adaptation. These results suggested the diverse degrees of miRNA transcriptome variation in different tissues between yak and cattle, and suggested extensive roles of miRNAs in high altitude adaptation.

Comparative analysis of the microRNA transcriptome between yak and cattle provides insight into high-altitude adaptation

PubMed Central

Zhang, Jinwei; He, Dafang; Jin, Long; Tang, Qianzi; Jiang, Anan; Wang, Xun; Hu, Yaodong; Tian, Shilin; Jiang, Zhi

2017-01-01

Extensive and in-depth investigations of high-altitude adaptation have been carried out at the level of morphology, anatomy, physiology and genomics, but few investigations focused on the roles of microRNA (miRNA) in high-altitude adaptation. We examined the differences in the miRNA transcriptomes of two representative hypoxia-sensitive tissues (heart and lung) between yak and cattle, two closely related species that live in high and low altitudes, respectively. In this study, we identified a total of 808 mature miRNAs, which corresponded to 715 pre-miRNAs in the two species. The further analysis revealed that both tissues showed relatively high correlation coefficient between yak and cattle, but a greater differentiation was present in lung than heart between the two species. In addition, miRNAs with significantly differentiated patterns of expression in two tissues exhibited co-operation effect in high altitude adaptation based on miRNA family and cluster. Functional analysis revealed that differentially expressed miRNAs were enriched in hypoxia-related pathways, such as the HIF-1α signaling pathway, the insulin signaling pathway, the PI3K-Akt signaling pathway, nucleotide excision repair, cell cycle, apoptosis and fatty acid metabolism, which indicated the important roles of miRNAs in high altitude adaptation. These results suggested the diverse degrees of miRNA transcriptome variation in different tissues between yak and cattle, and suggested extensive roles of miRNAs in high altitude adaptation. PMID:29109913

Recombinant Human Bone Morphogenetic Protein 6 Enhances Oocyte Reprogramming Potential and Subsequent Development of the Cloned Yak Embryos.

PubMed

Pan, Yangyang; He, Honghong; Cui, Yan; Baloch, Abdul Rasheed; Li, Qin; Fan, Jiangfeng; He, Junfeng; Yu, Sijiu

2015-12-01

This study investigated the effects of bone morphogenetic protein 6 (BMP6) supplementation in the medium during in vitro maturation (IVM) on the developmental potential of oocytes and in the subsequent development of cloned yak embryos. Cumulus-oocyte complexes (COCs) were aspirated from the antral follicles of yak ovaries and cultured with different concentrations of recombinant human BMP6 in oocyte maturation medium. Following maturation, the metaphase II (MII) oocytes were used for somatic cell nuclear transfer (SCNT), and these were cultured in vitro. The development of blastocysts and cell numbers were detected on day 8. The apoptosis and histone modifications of yak cloned blastocysts were evaluated by detecting the expression of relevant genes and proteins (Bax, Bcl-2, H3K9ac, H3K18ac, and H3K9me3) using relative quantitative RT-PCR or immunofluorescence. The presence of 100 ng/mL BMP6 significantly enhanced the oocyte maturation ratios (66.12 ± 2.04% vs. 73.11 ± 1.38%), cleavage rates (69.40 ± 1.03% vs. 78.16 ± 0.93%), and blastocyst formation rates (20.63 ± 1.32% vs. 28.16 ± 1.67%) of cloned yak embryos. The total blastocysts (85.24 ± 3.12 vs. 103.36 ± 5.28), inner cell mass (ICM) cell numbers (19.59 ± 2.17 vs. 32.20 ± 2.61), and ratio of ICM to trophectoderm (TE) (22.93 ± 1.43% vs. 31.21 ± 1.62%) were also enhanced (p < 0.05). The ratio of the Bax to the Bcl-2 gene was lowest in the SCNT + BMP6 groups (p < 0.05). The H3K9ac and H3K18ac levels were increased in SCNT + BMP6 groups (p < 0.05), whereas the H3K9me3 level was decreased; the differences in blastocysts were not significant (p > 0.05). These study results demonstrate that addition of oocyte maturation medium with recombinant BMP6 enhances yak oocyte developmental potential and the subsequent developmental competence of SCNT embryos, and provides evidence that BMP6 is an important determinant of mammalian oocyte developmental reprogramming.

Recombinant Human Bone Morphogenetic Protein 6 Enhances Oocyte Reprogramming Potential and Subsequent Development of the Cloned Yak Embryos

PubMed Central

Pan, Yangyang; He, Honghong; Cui, Yan; Baloch, Abdul Rasheed; Li, Qin; Fan, Jiangfeng; He, Junfeng

2015-01-01

Abstract This study investigated the effects of bone morphogenetic protein 6 (BMP6) supplementation in the medium during in vitro maturation (IVM) on the developmental potential of oocytes and in the subsequent development of cloned yak embryos. Cumulus–oocyte complexes (COCs) were aspirated from the antral follicles of yak ovaries and cultured with different concentrations of recombinant human BMP6 in oocyte maturation medium. Following maturation, the metaphase II (MII) oocytes were used for somatic cell nuclear transfer (SCNT), and these were cultured in vitro. The development of blastocysts and cell numbers were detected on day 8. The apoptosis and histone modifications of yak cloned blastocysts were evaluated by detecting the expression of relevant genes and proteins (Bax, Bcl-2, H3K9ac, H3K18ac, and H3K9me3) using relative quantitative RT-PCR or immunofluorescence. The presence of 100 ng/mL BMP6 significantly enhanced the oocyte maturation ratios (66.12 ± 2.04% vs. 73.11 ± 1.38%), cleavage rates (69.40 ± 1.03% vs. 78.16 ± 0.93%), and blastocyst formation rates (20.63 ± 1.32% vs. 28.16 ± 1.67%) of cloned yak embryos. The total blastocysts (85.24 ± 3.12 vs. 103.36 ± 5.28), inner cell mass (ICM) cell numbers (19.59 ± 2.17 vs. 32.20 ± 2.61), and ratio of ICM to trophectoderm (TE) (22.93 ± 1.43% vs. 31.21 ± 1.62%) were also enhanced (p < 0.05). The ratio of the Bax to the Bcl-2 gene was lowest in the SCNT + BMP6 groups (p < 0.05). The H3K9ac and H3K18ac levels were increased in SCNT + BMP6 groups (p < 0.05), whereas the H3K9me3 level was decreased; the differences in blastocysts were not significant (p > 0.05). These study results demonstrate that addition of oocyte maturation medium with recombinant BMP6 enhances yak oocyte developmental potential and the subsequent developmental competence of SCNT embryos, and provides evidence that BMP6 is an important determinant of mammalian oocyte developmental reprogramming. PMID:26655079

The health of populations living in the indigenous minority settlements of northern Yakutia.

PubMed

Burtseva, Tatiana E; Uvarova, Tatiana E; Tomsky, Mikhail I; Odland, Jon Ø

2014-01-01

This monograph contains the results of a study carried out by the Yakutsk Research Center for Complex Medical Problems, "Evaluating the health of the indigenous minorities of the Sakha Republic (Yakutia) and optimizing medical assistance using innovative technologies and telemedicine in indigenous settlements." The child population was studied in 19 indigenous minority settlements, and the adult population was studied in 12 settlements.

Experimental observations of strengthening the neutron flux during negative lightning discharges of thunderclouds with tripolar configuration

NASA Astrophysics Data System (ADS)

Toropov, A. A.; Kozlov, V. I.; Mullayarov, V. A.; Starodubtsev, S. A.

2013-03-01

We consider neutron bursts (Yakutsk cosmic ray spectrograph,105 m above sea level) and the electric field during lightning discharges. It was found that the neutron bursts are observed in the negative lightning discharg only. We discuss the possibility of generation of neutrons in the lower part (the point of impact into the ground) lightning discharge.

Effect of thawing methods on frozen semen quality of yak (Poephagus grunniens L.) bulls

PubMed Central

Borah, Binod Kumar Dutta; Deka, Bharat Chandra; Biswas, Ranjan Kumar; Chakravarty, Prithiviraj; Deori, Sourabh; Sinha, Sudip; Ahmed, Kutubuddin

2015-01-01

Aim: To evaluate different thawing temperatures and duration on the post-thaw semen quality of Indian yaks bulls. Materials and Methods: Semen ejaculates from four different yak bulls were collected using artificial vagina method and extended with tris extender containing 6.4% glycerol at 35°C, cooled gradually from 35°C to 5°C at 1°C/3 min and equilibrated at 4-5°C for 4 h and frozen in French mini straws using a programmable bio-freezer and finally stored in liquid nitrogen. Thawing of frozen semen straws was carried out using three methods i.e., 35°C for 60 s (thawing method I), 37°C for 30 s (thawing method II) and 75°C for 9 s (thawing method III). The post-thaw semen quality parameters assessed were sperm motility, percent live sperm, hypo-osmotic swelling test (HOST)-reacted sperm, acrosomal changes, and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in the extracellular media. Results: The percent sperm motility, total incidence of acrosomal changes, and extracellular release of AST varied significantly (p<0.01) between thawing methods but live sperm and HOST-reacted sperm did not vary significantly between thawing methods. The percent sperm motility of frozen yak semen for thawing method III was significantly (p<0.05) higher than that for thawing methods I and II, the difference between thawing methods I and II being non-significant. The critical difference test revealed that the total incidence of acrosomal changes and extracellular release of AST were significantly (p<0.05) lower when thawing was done using methods I and II than in method III. Conclusion: On the basis of the present experiment, we can conclude that barring the post-thaw sperm motility, thawing of frozen yak semen in water either at 35°C for 60 s or 37°C for 30 s gives better post-thaw semen quality than at 75°C for 09 s. PMID:27047161

Molecular Characterization of Hypoderma SPP. in Domestic Ruminants from Turkey and Pakistan.

PubMed

Ahmed, Haroon; Simsek, Sami; Saki, Cem Ecmel; Kesik, Harun Kaya; Kilinc, Seyma Gunyakti

2017-08-01

The aim of this study was to determine the morphological and molecular characterization of Hypoderma spp. in cattle and yak from provinces in Turkey and Pakistan. In total, 78 Hypoderma larvae were collected from slaughtered animals in Turkey and Pakistan from October 2015 to January 2016. Thirty-eight of these 78 Hypoderma larvae were morphologically classified as third instar larvae (L3s) of Hypoderma bovis, 37 were classified as Hypoderma lineatum, and 3 were classified as suspected or unidentified. The restriction enzyme TaqI was used to differentiate the Hypoderma spp. by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). According to the sequences and the PCR-RFLP results, all larval samples from cattle from Turkey were classified as H. bovis, except for 1 sample classified as H. lineatum. All Hypoderma larvae from Pakistan were classified as H. lineatum from cattle and as Hypoderma sinense from yak. This study provides the first molecular characterization of H. lineatum (cattle) and H. sinense (yak) in Pakistan based on PCR-RFLP and sequencing results.

Study on the effect of reactive oxygen species-mediated oxidative stress on the activation of mitochondrial apoptosis and the tenderness of yak meat.

PubMed

Wang, Lin-Lin; Yu, Qun-Li; Han, Ling; Ma, Xiu-Li; Song, Ren-De; Zhao, Suo-Nan; Zhang, Wen-Hua

2018-04-01

This study investigated the effect of reactive oxygen species-mediated oxidative stress on activation of mitochondrial apoptosis and tenderness of yak meat during postmortem ageing. Oxidative stress degree, Ca 2+ levels, membrane permeability transition pore opening, mitochondrial membrane potential, apoptotic factors and the shear force were examined. Results showed that the ROS generated by H 2 O 2 significantly increased mitochondrial oxidative stress by decreasing the activities of superoxide dismutase, catalase and glutathione peroxidase, and increasing lipid peroxidation. Furthermore, oxidative stress enhanced Ca 2+ production and cytochrome c release, changed the levels of Bcl-2 family proteins and activated caspase-9 and -3 activities. Ultimately, oxidative stress increased the apoptosis rate and tenderness of yak meat. These observations confirmed that ROS-mediated oxidative stress participates in the activation of the apoptotic cascade reaction involving Ca 2+ and Bcl-2 family proteins. The results further suggested that ROS-mediated oxidative stress plays a significant role in meat tenderization through the mitochondrial apoptotic pathway. Copyright © 2017. Published by Elsevier Ltd.

Structure elucidation of two novel yak milk oligosaccharides and their DFT studies

NASA Astrophysics Data System (ADS)

Singh, Ashish Kumar; Ranjan, Ashok Kr.; Srivastava, Gaurav; Deepak, Desh

2016-03-01

Milk is a primary dynamic biological fluid responsible for development of neonates. Besides the other regular constituents it have oligosaccharides in it which are responsible for antitumor, anticancer, antigenic and immunostimulant activities. In our endeavor to find biologically active novel oligosaccharides, yak milk was taken, which is a rich source of oligosaccharide and its milk is used as antihypertensive, antioxidative and heart strengthening agent in folk medicine. For this purpose yak milk was processed by method of Kobata and Ginsburg followed by gel filtration HPLC and CC which resulted in the isolation of two novel milk oligosaccharides namely (I) Grunniose and (II) Vakose. The structure of purified milk oligosaccharides were elucidated with the help of chemical degradation, chemical transformation, spectroscopic techniques like NMR (1H, 13C and 2D-NMR), structure reporter group theory and mass spectrometry. The optimized geometry of compound Grunniose and Vakose, at B3LYP method and 6-311 + G basis set on Gaussian 09 program, show that the compound Grunniose is lower in energy as compared to compound Vakose.

Different gene expressions between cattle and yak provide insights into high-altitude adaptation.

PubMed

Wang, K; Yang, Y; Wang, L; Ma, T; Shang, H; Ding, L; Han, J; Qiu, Q

2016-02-01

DNA sequence variation has been widely reported as the genetic basis for adaptation, in both humans and other animals, to the hypoxic environment experienced at high altitudes. However, little is known about the patterns of gene expression underlying such hypoxic adaptations. In this study, we examined the differences in the transcriptomes of four organs (heart, kidney, liver and lung) between yak and cattle, a pair of closely related species distributed at high and low altitudes respectively. Of the four organs examined, heart shows the greatest differentiation between the two species in terms of gene expression profiles. Detailed analyses demonstrated that some genes associated with the oxygen supply system and the defense systems that respond to threats of hypoxia are differentially expressed. In addition, genes with significantly differentiated patterns of expression in all organs exhibited an unexpected uniformity of regulation along with an elevated frequency of nonsynonymous substitutions. This co-evolution of protein sequences and gene expression patterns is likely to be correlated with the optimization of the yak metabolic system to resist hypoxia. © 2015 Stichting International Foundation for Animal Genetics.

Developmental competence of frozen-thawed yak (Bos grunniens) oocytes followed by in vitro maturation and fertilization.

PubMed

Niu, Hui-Ran; Zi, Xiang-Dong; Xiao, Xiao; Xiong, Xian-Rong; Zhong, Jin-Cheng; Li, Jian; Wang, Li; Wang, Yong

2014-02-01

In the present study, we examined the ability of immature germinal vesicle (GV) and subjected to in vitro matured (MII) yak oocytes to survive after cryopreservation as well as their subsequent development following in vitro maturation and fertilization. Both GV and MII oocytes were cryopreserved by using two different vitrification solutions (VS); VS-I contained 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in TCM-199 + 20% (v/v) fetal calf serum (FCS) whereas VS-II contained 40% EG + 18% Ficoll + 0.5 M sucrose in TCM-199 + 20% FCS. The percentage of oocytes found to be morphologically normal was greater (P < 0.01) in VS-I group than in VS-II group. Rates of cleavage (30.6-42.2%) and blastocyst formation (2.9-8.9%) did not differ among groups, but were lower than in unfrozen control (55.7% and 25.4%, P < 0.01). These results show that a combination of EG and DMSO or EG, Ficoll and sucrose can be used to cryopreserve yak oocytes in French straws. Copyright © 2014 Elsevier Inc. All rights reserved.

JPRS Report Proliferation Issues

DTIC Science & Technology

1991-12-02

Nuclear Arms [Sao Paulo FOLHA DE SAO PAULO 11 Nov] 36 Ukraine Official Views Defense Plans, Nuclear Weapons [Prague RUDE PRAVO 7Nov] 36 Yakutsk...1992 92WP0057A Sao Paulo GAZETA MERCANTIL in Portuguese 22 Oct 91 p 15 [Article by Sao Paulo correspondent Luis Leonel] [Text] The 1992 budget for...Misinterpretation He explained that Minister of the Infrastructure Joao Santana had been misinterpreted when he said two weeks ago that the

The health of populations living in the indigenous minority settlements of northern Yakutia

PubMed Central

Burtseva, Tatiana E.; Uvarova, Tatiana E.; Tomsky, Mikhail I.; Odland, Jon Ø.

2014-01-01

This monograph contains the results of a study carried out by the Yakutsk Research Center for Complex Medical Problems, “Evaluating the health of the indigenous minorities of the Sakha Republic (Yakutia) and optimizing medical assistance using innovative technologies and telemedicine in indigenous settlements.” The child population was studied in 19 indigenous minority settlements, and the adult population was studied in 12 settlements. PMID:25405106

Molecular characterization of fluoroquinolone and/or cephalosporin resistance in Shigella sonnei isolates from yaks.

PubMed

Zhu, Zhen; Shi, Yuxiang; Zhou, Xuzheng; Li, Bing; Zhang, Jiyu

2018-06-07

Members of the genus Shigella are intestinal pathogens and a major cause of seasonal outbreaks of bacterial diarrhea worldwide. Although humans are the conventional hosts of Shigella species, expansion of the Shigella host range to certain animals was recently reported. To investigate the prevalence of Shigella sonnei (S. sonnei) in yaks and perform molecular characterization, we analyzed 1132 fresh yak diarrheal stool samples and collected a total of 44 S. sonnei isolates. We performed multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) with XbaI-digested DNA to study genetic relatedness among the 44 isolates, which were differentiated into 4 sequence types (STs) and 32 PFGE types (PTs). All isolates harbored virulence genes, and 87.36% tested positive for invasion plasmid antigen H (ipaH), invasion associated locus (ial) and the Shigella enterotoxin gene sen. According to the results of antimicrobial susceptibility tests, 45.45% (20/44) were resistant to fluoroquinolones and/or cephalosporin. By sequencing the quinolone resistance determining region (QRDR) genes, we identified double mutations in gyrA (Ser83-Leu and Asp87-Asn) and a single mutation in parC (Ser80-Ile). All 12 fluoroquinolone-resistant S. sonnei isolates tested positive for the aac(6')-Ib-cr gene but negative for qepA. Three isolates harbored qnr genes, including two with qnrS and one with qnrB. In addition, three types of β-lactamase genes, bla TEM-1 , bla OXA-1 and bla CTX-M-14/79 , were detected in cephalosporin-resistant isolates. The findings of this study have enriched our knowledge of fluoroquinolone- and/or cephalosporin-resistant S. sonnei isolates from yaks, which has important public health significance.

Highly Conserved Keratin-Associated Protein 7-1 Gene in Yak, Taurine and Zebu Cattle.

PubMed

Arlud, S; He, N; Sari, E M; Ma, Z-J; Zhang, H; An, T-W; Han, J-L

2017-01-01

Keratin-associated proteins (KRTAPs) play a critical role in cross-linking the keratin intermediate filaments to build a hair shaft. The genetic polymorphisms of the bovine KRTAP7-1 gene were investigated for the first time in this study. The complete coding sequence of the KRTAP7-1 gene in 108 domestic yak, taurine and zebu cattle from China and Indonesia were successfully amplified using polymerase chain reaction and then directly sequenced. Only two single-nucleotide polymorphisms (one nonsynonymous at c.7C/G and another synonymous at c.21C/T) and three haplotypes (BOVIN-KRTAP7-1*A, B and C) were identified in the complete coding sequence of the bovine KRTAP7-1 gene among all animals. There was no polymorphism across three Chinese indigenous yak breeds and one Indonesian zebu cattle population, all sharing the BOVINKRTAP71*A haplotype. The four taurine cattle populations also had BOVIN-KRTAP7-1*A as the most common haplotype with a frequency of 0.80. The frequency of novel haplotype BOVIN-KRTAP7-1*B was only 0.07 present in one heterozygous animal in each of the four taurine cattle populations, while BOVINKRTAP7- 1*C was only found in a Simmental and a local Chinese Yellow cattle population with frequencies of 0.17 and 0.36, respectively. The monomorphic yak KRTAP7-1 gene in particular, and highly conserved bovine, sheep and goat KRTAP7-1 genes in general, demonstrated its unique intrinsic structural property (e.g., > 21% high glycine content) and primary functional importance in supporting the mechanical strength and shape of hair.

Low-carbon yak cheese: transition to biogas in a Himalayan socio-technical niche

PubMed Central

Campbell, Ben; Sallis, Paul

2013-01-01

This study looks at how potential for resilient low-carbon solutions can be understood and enhanced in the diverse environmental, economic and socio-political contexts in which actual scenarios of energy needs and diverse development pathways take shape. It discusses socio-technical transition approaches to assist implementation of a biogas digester system. This will replace fuelwood use in the high forests of Central Nepal, where yak cheese production provides livelihood income but is under threat from the Langtang National Park, which is concerned to protect biodiversity. Alternatives for digester design are discussed, and the consultative issues for deliberative processes among stakeholders’ varied agendas raised. PMID:24427516

Regularities of changes in fluid composition and properties in Vankor field pools: from light to heavy oil

NASA Astrophysics Data System (ADS)

Goncharov, I. V.; Oblasov, N. V.

2015-02-01

Oil in layers Nkh 3-4, Nkh 1, Sd 9, Yak 3-7 and vYak 2-4 of the Vankor field occurs at the depth of -2,767 to -1,357 meters at strongly different temperatures: from 62 to 26 °C. Such temperature conditions contribute to oil biodegradation processes in the pool. Therefore, oils in different pools significantly differ from each other in terms of composition and properties depending on the intensity of biodegradation. At the same time, pools might embrace both oils that have practically been not exposed to biodegradation processes and significantly biodegraded oils. The most seriously altered oils are found in vYak 2-4 layer pools. They are the heaviest and the most viscous oils among the samples under study. Many typical oil components (alkanes, alkylbenzenes, naphthalenes, phenanthrenes, dibenzothiophenes) are absent in their composition. Besides, the initial distribution of hopanes in the composition of biomarkers is altered. Apart from the molecular composition of degassed oil samples, the work also studies the effect of biodegradation on the properties and the component and isotopic composition of oils, gases and formation fluid samples.

Effect of pH on dissociation of casein micelles in yak skim milk.

PubMed

Yang, M; Zhang, G D; Yang, J T; Sun, D; Wen, P C; Zhang, W B

2018-04-01

The dissociation of yak casein (CN) micelles was evaluated by scanning electron microscopy, particle size, fluorescence properties, and soluble mineral and CN molecule content at pH 4.6 to 8.2. The results showed that the size of CN micelles remained constant with decreasing pH from 8.2 to 5.8 but sharply increased at pH ≤5.4. Casein micelles began to aggregate at pH 5.4, and the serum magnesium, potassium, iron, zinc, copper, and manganese levels had their minimum values at this pH level. During acidification, colloidal calcium phosphate dramatically disassociated from yak CN micelles, but the soluble CN monomer content decreased slightly. During alkalization, the soluble calcium and phosphorus content decreased below pH 6.8 but increased with pH increases from 6.8 to 8.2. However, the soluble CN content increased markedly during alkalization. The emission wavelength of 8-anilino-1-naphthalenesulfonic acid sodium salt fluorescence decreased during both acidification and alkalization from pH 6.6, whereas the opposite was found for intrinsic fluorescence. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Cosmic rays energy determination by radio emission registration method at frequency 30-35 MHz

NASA Astrophysics Data System (ADS)

Knurenko, S. P.; Petrov, I. S.

2017-11-01

The study of cosmic rays (CR) of ultrahigh energies first of all requires an estimate of the energy, the spectrum in terms of energies, masses, and the anisotropy of the arrival of primary particles. This can only be done by calculating the energies of all the secondary particles formed during the interaction of the primary particle with the nuclei of the air atoms [1]. First of all, this is the registration of Cherenkov and ionization radiation [2, 3]. According to model calculations, the loss of ionization of air by particles is spent up to 80% of the total energy of the primary particle [4]. The rest of the energy is spent on nuclear interactions of hadrons (nucleons) and is carried away by high-energy muons at sea level. In the hybrid registration of air showers, i.e. electron, muon, and Cherenkov components, we can empirically estimate the energy of the primary CR particle. Such a method has been developed and is being used to this day at the Yakutsk complex installation of the EAS. A detailed description of this method is given in [5, 6]. As an alternative to the energy balance method, we describe below the method of independent estimation of E0 obtained by measuring the radio emission of EAS at the frequency of 30-35 MHz at the Yakutsk array.

Strong tolerance to freezing is a major survival strategy in insects inhabiting central Yakutia (Sakha Republic, Russia), the coldest region on earth.

PubMed

Li, N G

2016-10-01

Yakutia is a part of eastern Siberia, located in north-eastern Russia. The climate of this area is very harsh even by Siberian standards, and is characterized by the absolute temperature minimum, which is below -64.4 °C, and a long period of low temperatures reaching to a range between -47 and -55 °C. Despite such a severe climate, the fauna and flora of Yakutia present a considerably rich biodiversity, suggesting a high adaptation potential of the organisms in this area. In this study, 30 local species of insects belonging to Coleoptera, Diptera and Lepidoptera were selected to investigate cold adaptation. The identification of the cold adaptation strategy was based on the measurement of the insect body supercooling point (SCP) and hemolymph ice-nucleating activity. According to the data collected, there is a high incidence of freeze tolerant species among the insects found in Yakutsk area (Yakutsk, 62° latitude, 130° longitude): 93.3% of them were freeze tolerant, and only 6.7% were freeze avoiding. It is suggested that the evolution of cold hardiness in this region preferably develops for the selection of the strong freeze tolerance that allow the insects to survive extreme cold conditions. Copyright © 2016 Elsevier Inc. All rights reserved.

Splice variants and promoter methylation status of the Bovine Vasa Homology (Bvh) gene may be involved in bull spermatogenesis

PubMed Central

2013-01-01

Background Vasa is a member of the DEAD-box protein family that plays an indispensable role in mammalian spermatogenesis, particularly during meiosis. Bovine vasa homology (Bvh) of Bos taurus has been reported, however, its function in bovine testicular tissue remains obscure. This study aimed to reveal the functions of Bvh and to determine whether Bvh is a candidate gene in the regulation of spermatogenesis in bovine, and to illustrate whether its transcription is regulated by alternative splicing and DNA methylation. Results Here we report the molecular characterization, alternative splicing pattern, expression and promoter methylation status of Bvh. The full-length coding region of Bvh was 2190 bp, which encodes a 729 amino acid (aa) protein containing nine consensus regions of the DEAD box protein family. Bvh is expressed only in the ovary and testis of adult cattle. Two splice variants were identified and termed Bvh-V4 (2112 bp and 703 aa) and Bvh-V45 (2040 bp and 679 aa). In male cattle, full-length Bvh (Bvh-FL), Bvh-V4 and Bvh-V45 are exclusively expressed in the testes in the ratio of 2.2:1.6:1, respectively. Real-time PCR revealed significantly reduced mRNA expression of Bvh-FL, Bvh-V4 and Bvh-V45 in testes of cattle-yak hybrids, with meiotic arrest compared with cattle and yaks with normal spermatogenesis (P < 0.01). The promoter methylation level of Bvh in the testes of cattle-yak hybrids was significantly greater than in cattle and yaks (P < 0.01). Conclusion In the present study, Bvh was isolated and characterized. These data suggest that Bvh functions in bovine spermatogenesis, and that transcription of the gene in testes were regulated by alternative splice and promoter methylation. PMID:23815438

Molecular Epidemiological Survey and Genetic Characterization of Anaplasma Species in Mongolian Livestock.

PubMed

Ochirkhuu, Nyamsuren; Konnai, Satoru; Odbileg, Raadan; Murata, Shiro; Ohashi, Kazuhiko

2017-08-01

Anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. Few studies on Anaplasma infections in Mongolian livestock have been conducted. This study examined the prevalence of Anaplasma marginale, Anaplasma ovis, Anaplasma phagocytophilum, and Anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (Bos taurus), yaks (Bos grunniens), sheep (Ovis aries), and goats (Capra aegagrus hircus) in four provinces of Ulaanbaatar city in Mongolia. We genetically characterized positive samples through sequencing analysis based on the heat-shock protein groEL, major surface protein 4 (msp4), and 16S rRNA genes. Only A. ovis was detected in Mongolian livestock (cattle, yaks, sheep, and goats), with 413 animals (44.5%) positive for groEL and 308 animals (33.2%) positive for msp4 genes. In the phylogenetic tree, we separated A. ovis sequences into two distinct clusters based on the groEL gene. One cluster comprised sequences derived mainly from sheep and goats, which was similar to that in A. ovis isolates from other countries. The other divergent cluster comprised sequences derived from cattle and yaks and appeared to be newly branched from that in previously published single isolates in Mongolian cattle. In addition, the msp4 gene of A. ovis using same and different samples with groEL gene of the pathogen demonstrated that all sequences derived from all animal species, except for three sequences derived from cattle and yak, were clustered together, and were identical or similar to those in isolates from other countries. We used 16S rRNA gene sequences to investigate the genetically divergent A. ovis and identified high homology of 99.3-100%. However, the sequences derived from cattle did not match those derived from sheep and goats. The results of this study on the prevalence and molecular characterization of A. ovis in Mongolian livestock can facilitate the control of infectious diseases in livestock.

Colony-stimulating factor 2 enhances the developmental competence of yak (Poephagus grunniens) preimplantation embryos by modulating the expression of heat shock protein 70 kDa 1A.

PubMed

Wen, Zexing; Pan, Yangyang; Cui, Yan; Peng, Xiumei; Chen, Ping; Fan, Jiangfeng; Li, Guyue; Zhao, Tian; Zhang, Jian; Qin, Shujian; Yu, Sijiu

2017-04-15

Colony-stimulating factor 2 (CSF 2 ) is known to promote the development and survival of rodents and ruminants preimplantation embryos; however, the effect of CSF 2 on yak embryos has not been reported. The objective of this study was to investigate the effects of CSF 2 on the developmental competence of yak embryos cultured in vitro in modified synthetic oviduct fluid (mSOF) medium and on the expression pattern of heat shock protein 70 kDa 1A (HSPA1A). In each experiment, cumulus-oocyte complexes (COCs) were matured in vitro and fertilized with frozen-thawed semen. Zygotes were treated with varying concentrations of CSF 2 (0, 10, 50, 100 ng/mL) until day 8 after fertilization. Embryo development was calculated as the percentage of oocytes that formed embryos at the 2-cell, 4-cell, 8-cell, 16-cell, morula and blastocyst stages. The total cell numbers (TCN) per blastocyst and their allocation to the inner cell mass (ICM) and trophectoderm (TE) lineages were determined using differential CDX2 staining. The expression of HSPA1A was examined by quantitative real-time PCR (qRT-PCR) and immunochemistry to determine the mRNA and protein levels. The results showed that treatment with 50 ng/mL CSF 2 significantly (P < 0.05) increased the rate of blastocyst formation (19.01% versus 9.93%) and the TCN per blastocyst (96.94 versus 81.41) compared to the control group. However, no significant differences were observed in the other stages of development. qRT-PCR analysis confirmed that treatment with 50 ng/mL CSF 2 significantly (P < 0.05) inhibited the expression of HSPA1A mRNA in blastocysts cultured in vitro relative to the control group, but there were no significant differences between the other treatment groups. Immunocytochemical analysis confirmed that HSPA1A protein accumulation was gradually reduced in yak blastocysts cultured in 0, 10, 100 or 50 ng/mL CSF 2 , however, no significant differences were observed between the 10 and 100 ng/mL treatments (P > 0.05). In conclusion, these findings demonstrate that CSF 2 inhibits the expression of HSPA1A to facilitate yak blastocyst formation and increase cell numbers. Copyright © 2017. Published by Elsevier Inc.

Effects of the Yakutat terrane collision with North America on the neighboring Pacific plate

NASA Astrophysics Data System (ADS)

Reece, R.; Gulick, S. P.; Christeson, G. L.; Barth, G. A.; van Avendonk, H.

2011-12-01

High-resolution bathymetry data show a 30 km N-S trending ridge within the deep-sea Surveyor Fan between the mouths of the Yakutat Sea Valley and Bering Trough in the Gulf of Alaska. The ridge originates in the north, perpendicular to and at the base of the continental slope, coincident with the Transition Fault, the strike-slip boundary between the Yakutat terrane (YAK) and the Pacific plate (PAC). The ridge exhibits greatest relief adjacent to the Transition Fault, and becomes less distinct farther from the shelf edge. Seismic reflection data reveal a sharp basement high beneath the ridge (1.1 sec of relief above "normal" basement in two-way travel time) as well as multiple similarly oriented strike-slip fault segments. The ridge, basement high, and faults are aligned and co-located with an intraplate earthquake swarm on the PAC, which includes four events > 6.5 Mw that occurred from 1987-1992. The swarm is defined by right-lateral strike-slip events, and is collectively called the Gulf of Alaska Shear Zone (GASZ). Based on the extent of historic seismicity, the GASZ extends at least 230 km into the PAC, seemingly ending at the Kodiak-Bowie Seamount Chain. Farther southwest, between the Kodiak-Bowie and Patton-Murray Seamount Chains, there is a large regional bathymetric low with an axis centered along the Aja Fracture Zone, perpendicular to the GASZ and Aleutian Trench. Basement and overlying sediment in the low are irregularly, but pervasively faulted. The GASZ and faulted bathymetric low could represent PAC deformation due to PAC-YAK coupling whereby YAK resistance to subduction is expressed as deformation in the thinner (weaker) PAC crust. The YAK is an allochthonous, basaltic terrane coupled to the PAC that began subducting at a low angle beneath North America (NA) ~25-40 Ma. Due to its 15-25 km thickness, the YAK is resistant to subduction compared to the normal oceanic crust of the PAC. As a result the plates developed differential motion along the Transition Fault and have different, convergent, vectors for motion relative to NA. Although a tear on the scale of the GASZ in normal oceanic crust is unusual, preexisting zones of weakness, such as the Aja Fracture Zone and bending faults at the flexural bulge, may have proven to be a kinematically favorable localization for strain. These results expand on a previous tectonic model wherein the differing YAK and PAC vectors caused the northern PAC to behave as two tectonic blocks, separated by the GASZ. In this model, the eastern block of the PAC exhibits a counter-clockwise rotation that accounts for motion along the Transition Fault and GASZ. We will analyze seismic reflection, bathymetric, magnetic, and gravity data in order to further investigate this intraplate deformation and the cause of strain localization in both areas. New bathymetric and 2D seismic reflection data will allow us to confirm whether the GASZ previously extended beyond the Kodiak-Bowie Seamount Chain and the current zone of active seismicity, as well as to characterize the GASZ at opposite ends.

Isolation, structure elucidation and DFT study on two novel oligosaccharides from yak milk

NASA Astrophysics Data System (ADS)

Singh, Meenakshi; Kumar, Alok; Srivastava, Gaurav; Deepak, Desh; Singh, M. P. V. V.

2016-08-01

Two novel oligosaccharides were isolated from yak milk. The milk was processed by the method of Kobata and Ginsberg involving deproteination, centrifugation and lyophilization followed by gel filtrate chromatography acetylation and silica gel column chromatography of derivatized oligosaccharides while their homogeneity was confirmed by HPLC. The structures of these isolated oligosaccharides were elucidated by chemical transformation, chemical degradation, 1H, 13C NMR, 2D NMR (COSY, TOCSY and HSQC) and mass spectrometry. The geometry of compound A (Bosiose) and B (Bovisose) have been optimized at B3LYP method and 6-311 + G(d,p) basis set. The difference between the energies of A and B is 1.269 a.u. or 796.309 kcal/mol.

[Features of arterial blood pressure in elderly persons of different ethnic groups in Yakutsk].

PubMed

Nikitin, Iu P; Tatarinova, O V; Neustroeva, V N; Shcherbakova, L V; Sidorov, A S

2013-01-01

The differences in arterial blood pressure in the sample of population in the age of 60 and older of different ethnic groups in Yakutsk, as well as its connection with the other cardiovascular diseases risk factors have been analyzed. It was shown that the average values of systolic and diastolic blood pressure in subsample of the Yakuts appeared to be lower than in Caucasoid gerontic persons. The average values of systolic arterial blood pressure both in the Yakuts and in the Caucasoids were detected higher than normal values in all age-dependent subgroups. The average values of diastolic blood pressure in both ethnic groups were within the limits of high normal level. From 60 to 90 years and older the decrease in systolic and diastolic arterial blood pressure was detected; it was more marked in Caucasoid gerontic persons. The average values of pulse pressure in the Yakuts and in the Caucasoids appeared to be higher than the existing standard and didn't have any differences in ethnic groups. In both ethnical subsamples, pulse pressure values increase was observed in persons of 60-89 years old and its decrease after 90. Persons with overweight, obesity, central (abdominal) obesity, dyslypoproteidemias irrespective of belonging to ethnical group were characterized as having higher levels of arterial blood pressure. Statistically significant differences in the levels of arterial blood pressure in the Yakuts and in the Caucasoids depending on hyperglycemia, smoking, the presence of burdened anamnesis, educational level, marital status was not detected.

Estimate of temperature change due to ice and snow accretion in the boreal forest regions

NASA Astrophysics Data System (ADS)

Sugiura, K.; Nagai, S.; Suzuki, R.; Eicken, H.; Maximov, T. C.

2016-12-01

Previous research has demonstrated that there is a wide difference between the surface albedo in winter/spring in snow-covered forest regions in various global climate models. If the forest is covered with snow, the surface albedo would increase. In this study, we carried out field observations to monitor the frequency of ice and snow accretion in the boreal forest regions. The time-lapse digital camera was set up on each side of the observation towers at the site located to the north of Fairbanks (USA) and at the site located to the north of Yakutsk (Russia). It was confirmed that both forests were not necessarily covered with snow without a break from the start of continuous snow cover until the end. In addition, the boreal forest at the Yakutsk site is covered with snow in comparison with the boreal forest at the Fairbanks site for a long term such as for about five month. Using a one-dimensional mathematics model about the energy flow including atmospheric multiple scattering, we estimated temperature change due to ice and snow accretion in the boreal forest regions. The result show that the mean surface temperature rises approximately 0.5 [oC] when the boreal forest is not covered with snow. In this presentation, we discuss the snow albedo parameterization in the boreal forest regions and the one-dimensional mathematics model to provide a basis for a better understanding of the role of snow in the climate system.

Atmospheric methane over Siberia: measurements from the 2014 YAK-AEROSIB aircraft campaign

NASA Astrophysics Data System (ADS)

Paris, Jean-Daniel; Pisso, Ignacio; Ancellet, Gérard; Law, Kathy; Arshinov, Mikhail Yu.; Belan, Boris D.; Nédélec, Philippe; Myhre, Cathrine Lund

2017-04-01

The YAK-AEROSIB program collects high-precision in-situ measurements of the vertical distribution of CO2, CH4, CO, O3, black carbon and ultrafine particles distribution in the Siberian troposphere, as well as other parameters including aerosol lidar profiles, on a pan-Siberian aircraft transect. Recent efforts aim at better understanding the respective role of CH4 emission processes in driving its large scale atmospheric variability over the region. The October 2014 YAK-AEROSIB/MOCA campaign from Novosibirsk to Salekhard and over the Kara sea and the Yamal peninsula sampled air masses affected by local, regional and remote pollution. We analyse the contribution of local anthropogenic sources to measured CH4 enhancements, in relation to atmospheric mixing and transport conditions. Our analysis also attempts to detect CH4 signal from sources of methane in the Siberian shelf and the Arctic ocean during low level flight legs over the Kara sea using the airborne measurements and a Lagrangian model coupled to potential CH4 hydrate and geological sources. The measured CH4 concentrations do not contradict a potential source upstream of our measurements, but the interpretation is challenging due to a very low CH4 signal. The challenging question of the methane budget and its evolution in Siberia leads to a need for new approaches. A new generation of airborne measurements, more flexible, is now needed.

Hydrological and sedimentary controls over fluvial thermal erosion, the Lena River, central Yakutia

NASA Astrophysics Data System (ADS)

Tananaev, Nikita I.

2016-01-01

Water regime and sedimentary features of the middle Lena River reach near Yakutsk, central Yakutia, were studied to assess their control over fluvial thermal erosion. The Lena River floodplain in the studied reach has complex structure and embodies multiple levels varying in height and origin. Two key sites, corresponding to high and medium floodplain levels, were surveyed in 2008 to describe major sedimentary units and properties of bank material. Three units are present in both profiles, corresponding to topsoil, overbank (cohesive), and channel fill (noncohesive) deposits. Thermoerosional activity is mostly confined to a basal layer of frozen channel fill deposits and in general occurs within a certain water level interval. Magnitude-frequency analysis of water level data from Tabaga gauging station shows that a single interval can be deemed responsible for the initiation of thermal action and development of thermoerosional notches. This interval corresponds to the discharges between 21,000 and 31,000 m3 s- 1, observed normally during spring meltwater peak and summer floods. Competence of fluvial thermal erosion depends on the height of floodplain level being eroded, as it acts preferentially in high floodplain banks. In medium floodplain banks, thermal erosion during spring flood is constrained by insufficient bank height, and erosion is essentially mechanical during summer flood season. Bank retreat rate is argued to be positively linked with bank height under periglacial conditions.

Estimation of composition of cosmic rays with E sub zero approximately equals 10(17) - 10(18) eV

NASA Technical Reports Server (NTRS)

Glushkov, A. V.; Efimov, N. N.; Efremov, N. N.; Makarov, I. T.; Pravdin, M. I.; Dedenko, L. I.

1985-01-01

Fluctuations of the shower maximum depth obtained from analysis of electron and muon fluctuations and the extensive air showers (EAS) Cerenkov light on the Yakutsk array data and data of other arrays are considered. On the basis of these the estimation of composition of primaries with E sub 0 = 5.10 to the 17th power eV is received. Estimation of gamma-quanta flux with E sub 0 10 to the 17th power eV is given on the poor-muon showers.

Comparative Study on the Genetic Diversity of GHR Gene in Tibetan Cattle and Holstein Cows.

PubMed

Deng, Feilong; Xia, Chenyang; Jia, Xianbo; Song, Tianzeng; Liu, Jianzhi; Lai, Song-Jia; Chen, Shi-Yi

2015-01-01

Due to the phenotype-based artificial selection in domestic cattle, the underlying functional genes may be indirectly selected and show decreasing diversity in theory. The growth hormone receptor (GHR) gene has been widely proposed to significantly associate with critical economic traits in cattle. In the present study, we comparatively studied the genetic diversity of GHR in Tibetan cattle (a traditional unselected breed, n = 93) and Chinese Holstein cow (the intensively selected breed, n = 94). The Tibetan yak (n = 38) was also included as an outgroup breed. A total of 21 variants were detected by sequencing 1279 bp genomic fragments encompassing the largest exon 9. Twelve haplotypes (H1∼H12) constructed by 15 coding SNPs were presented as a star-like network profile, in which haplotype H2 was located at the central position and almost occupied by Tibetan yaks. Furthermore, H2 was also identical to the formerly reported sequence specific to African cattle. Only haplotype H5 was simultaneously shared by all three breeds. Tibetan cattle showed higher nucleotide diversity (0.00215 ± 0.00015) and haplotype diversity (0.678 ± 0.026) than Holstein cow. Conclusively, we found Tibetan cattle have retained relatively high genetic variation of GHR. The predominant presence of African cattle specific H2 in the outgroup yak breed would highlight its ancestral relationship, which may be used as one informative molecular marker in the phylogenetic studies.

Space weather and myocardial infarction diseases at subauroral latitudes

NASA Astrophysics Data System (ADS)

Samsonov, Sergey; Kleimenova, Natalia; Petrova, Palmira

The relationship of the number of calls for the emergency medical care in Yakutsk (subauroral latitudes) in connection with myocardial infarction diseases during years near the maximum (1992) and minimum (1998) of the 11-year geomagnetic disturbance cycle to space weather parameters has been studied. It is found that at subauroral latitudes, the increase of geomagnetic activity, namely, the occurrence of night magnetospheric substorms, plays the important role in the exacerbation of myocardial infarctions. Substorms are accompanied by Pi1 irregular geomagnetic pulsations with periods of (0.5-3.0) Hz, coinciding with heart rhythms of a human being, thus, these waves can be a biotropic factor negatively influencing on the occurrence of myocardial infarctions. The comparison of seasonal change of the number of calls for emergency medical care to patients at subauroral latitudes with a simultaneous seasonal change of fatal endings because of an infarction at low latitudes (Bulgaria) has shown their essential difference. Thus, in Bulgaria the maximum of infarctions have been marked in winter, and minimum - in summer, and in Yakutsk a few maxima coinciding with the sharp and considerable increases of the level of the planetary geomagnetic disturbances have been observed. In this case, in Bulgaria the infarctions could be connected with availability of the Pc1 geomagnetic pulsations. Thus, the stable quasi-sinusoidal Pc1 pulsations can be a biotropic factor influencing on the development of myocardial infarctions at middle latitudes and the Pi1 irregular geomagnetic pulsations, which do not propagate to the lower latitudes, could be a biotropic factor at subauroral latitudes.

Magnetic and Ionospheric Observations in the Far Eastern Region of Russia During the Magnetic Storm of 5 April 2010

NASA Astrophysics Data System (ADS)

Baishev, D. G.; Moiseyev, A. V.; Boroyev, R. N.; Kobyakova, S. E.; Stepanov, A. E.; Mandrikova, O. V.; Solovev, I. S.; Khomutov, S. Yu.; Polozov, Yu. A.; Yoshikawa, A.; Yumoto, K.

2015-12-01

Magnetic and ionospheric disturbances in the far eastern region of Russia during the magnetic storm of 5 April 2010 are studied using data of geophysical stations operated by IKFIA SB RAS and IKIR FEB RAS. By performing wavelet analysis of experimental data, the wavelet powers of geomagnetic perturbations at different stations are estimated, in an attempt to investigate the dynamical development of a geomagnetic storm. It is shown that, though weak geomagnetic disturbances were present prior to the main phase of magnetic storm, the variations of the magnetic field during a storm development were found to be rather strong. The highest intensity of geomagnetic disturbances during the interplanetary shock at the Earth's magnetosphere was observed at KTN (L~9) while at ZYK (L~4) strongest geomagnetic perturbations occurred during the magnetospheric substorm with the onset at 09:03 UT. Large geomagnetic fluctuations were recorded at TIX and CHD (L~5-6), when the High-Intensity Long-Duration Continuous AE Activity (HILDCAA) was observed on 6 April 2010. Ionospheric conditions at YAK (L~3.4) and PET (L~2.2) were characterized by a pre-storm enhancement in the electron density in the F2 layer on 4 April 2010 and prolonged negative phase of the ionospheric storm during the main and recovery phases of magnetic storm on 6-8 April 2010. These experimental results underscore the importance of multi-instrumental observations and provide clues to the complex interactive processes.

Complete genome sequence of Bacillus velezensis LM2303, a biocontrol strain isolated from the dung of wild yak inhabited Qinghai-Tibet plateau.

PubMed

Chen, Liang

2017-06-10

Bacillus velezensis LM2303 is a biocontrol strain with a broad inhibitory spectrum against plant pathogens, isolated from the dung of wild yak inhabited Qinghai-Tibet plateau, China. Here we present its complete genome sequence, which consists of a single, circular chromosome of 3,989,393bp with a 46.68% G+C content. Genome analysis revealed genes encoding specialized functions for the biosynthesis of antifungal metabolites and antibacterial metabolites, the promotion of plant growth, the alleviation of oxidative stress and nutrient utilization. And the biosynthesis of antimicrobial metabolites in strain LM2303 was confirmed by biochemical analysis, while its plant growth promoting traits were confirmed by inoculation tests. Our results will establish a better foundation for further studies and biocontrol application of B. velezensis LM2303. Copyright © 2017 Elsevier B.V. All rights reserved.

Temporal correlation between dyke swarms and crustal extension in the middle Palaeozoic Vilyui rift basin, Siberian platform

NASA Astrophysics Data System (ADS)

Polyansky, Oleg P.; Prokopiev, Andrei V.; Koroleva, Olga V.; Tomshin, Mikhail D.; Reverdatto, Vladimir V.; Selyatitsky, Alexander Yu.; Travin, Alexei V.; Vasiliev, Dmitry A.

2017-06-01

This paper presents results from new 40Ar/39Ar isotope dating of nine mafic dykes from three large dyke swarms (Vilyui-Markha, Kontai-Dzherba, and Chara-Sinsk) of the Yakutsk-Vilyui large igneous province (LIP), in addition to a reconstruction of the subsidence history of the middle Palaeozoic Vilyui paleorift basin (eastern Siberian platform). All previously published 40Ar/39Ar and U-Pb dates are summarized. Statistical analysis of the dyke ages reveals repeated magmatic events in the study area. Two major pulses of mafic magmatism are identified: one at the Frasnian-Famennian boundary, with a main peak at ca. 374.1 Ma, and another in the latest Devonian with a peak at ca. 363.4 Ma. The time of maximum intensity of dyke intrusion coincides (within uncertainty) with rapid subsidence in the Vilyui basin. The minimum total volume of middle Palaeozoic magmatism produced in the Yakutsk-Vilyui LIP is 100-215 K km3, which is much less than earlier estimates. Most of the mafic material within the Yakutsk-Vilyui LIP is related to the Vilyui basin and associated dyke swarms. Backstripping analysis of sedimentation in depressions of the Vilyui basin was carried out. Estimates were obtained for the spatial distribution of the stretching factor of the crust and mantle lithosphere, averaging 1.17 and 1.44, respectively. The amount of extension due to dyke intrusion is estimated to be 6%. Backstripping analysis of sedimentation in the Vilyui basin was used to assess the effect of both intraplate far-field forces and upwelling magma flows initiated by a mantle plume. A numerical thermomechanical model was developed to investigate the relations between two possible mechanisms by which the Vilyui rift was initiated: intraplate extension (passive rifting) and the ascent of a mantle magmatic diapir (active rifting). A model considering both of these mechanisms shows the contribution of the far-field extension forces and the effect of convective flows around the mantle plume, assuming a temperature of 1500 °C for a heat source operating for ≥ 10 Myr. The best-fit model predicts the formation of the Vilyui basin under extensional conditions with a depth-integrated horizontal driving force of 2.0 × 1013 N/m, which corresponds to a lithostatic pressure of 0.73. Appendix B. Numerical model; Appendix C, Table S1. Major and trace element average data for reference materials W-1, JB-3 and internal standard ST-1A. Table S2. Laboratory precision (Diamond and Precious Metal Geology Institute, Siberian Branch of Russian Academy of Sciences). Table S3. Chemical composition of plagioclase from samples dated in this work. Table S4. Chemical composition of clinopyroxene from samples dated in this work.

Identifying active structures in the Kayak Island and Pamplona Zones: Implications for offshore tectonics of the Yakutat Microplate, Gulf of Alaska

NASA Astrophysics Data System (ADS)

Worthington, Lindsay L.; Gulick, Sean P. S.; Pavlis, Terry L.

Within the northern Gulf of Alaska, the Yakutat (YAK) microplate obliquely collides with and subducts beneath the North American (NA) continent at near-Pacific plate velocities. We investigate the extent that thin-skinned deformation on offshore structures located within the western portion of the unsubducted YAK block accommodates YAK-NA convergence. We compare faulting and folding observed on high-resolution and basin-scale multichannel seismic (MCS) reflection data with earthquake locations and surface ruptures observed on high-resolution bathymetric data. Holocene sediments overlying the Kayak Island fault zone (KIZ), previously interpreted as a region of active contraction, are relatively flat-lying, suggesting that active convergence within the KIZ is waning. Seismic reflection profiles east of KIZ show up to ˜200 m of undisturbed sediments overlying older folds in the Bering Trough, indicating that this area has been tectonically inactive since at least the last ˜1.3 Ma. Farther east, MCS profiles image active deformation in surface sediments along the eastern edge of the Pamplona zone (PZ) fold-and-thrust belt, that are collocated with a concentration of earthquake events that continues southwest to Khitrov Ridge and onshore through Icy Bay. These observations suggest that during the late Quaternary offshore shallow deformation style changed from distributed across the western Yakutat block to localized at the eastern edge of the PZ with extrusion of sediments southwest through the Khitrov Ridge area to the Aleutian Trench. This shallow deformation is interpreted as deformation of an accretionary complex above a shallow decollement.

Epigenetic Regulation of Bovine Spermatogenic Cell-Specific Gene Boule

PubMed Central

Luo, Hua; Xu, Hongtao; Pan, Zengxiang; Xie, Zhuang; Li, Qifa

2015-01-01

Non-primate mammals have two deleted azoospermia (DAZ) family genes, DAZL and Boule; genes in this family encode RNA-binding proteins essential for male fertility in diverse animals. Testicular DAZL transcription is regulated by epigenetic factors such as DNA methylation. However, nothing is known about the epigenetic regulation of Boule. Here, we explored the role of DNA methylation in the regulation of the bovine Boule (bBoule) gene. We found that a long CpG island (CGI) in the bBoule promoter was hypermethylated in the testes of cattle-yak hybrids with low bBoule expression, whereas cattle had relatively low methylation levels (P < 0.01), and there was no difference in the methylation level in the short CGI of the gene body between cattle and cattle-yak hybrids (P > 0.05). We identified a 107 bp proximal core promoter region of bBoule. Intriguingly, the differences in the methylation level between cattle and cattle-yak hybrids were larger in the core promoter than outside the core promoter. An in vitro methylation assay showed that the core promoter activity of bBoule decreased significantly after M.SssI methylase treatment (P < 0.01). We also observed dramatically increased bBoule transcription in bovine mammary epithelial cells (BMECs) after treatment with the methyltransferase inhibitor 5-Aza-dC. Taken together, our results establish that methylation status of the core promoter might be involved in testicular bBoule transcription, and may provide new insight into the epigenetic regulation of DAZ family genes and clinical insights regarding male infertility. PMID:26030766

Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen

PubMed Central

Song, Lei; Liu, Di; Liu, Li; Chen, Furong; Wang, Min; Li, Jiabao; Zeng, Xiaowei; Dong, Zhiyang; Hu, Songnian; Li, Lingyan; Xu, Jian; Huang, Li; Dong, Xiuzhu

2012-01-01

The rumen hosts one of the most efficient microbial systems for degrading plant cell walls, yet the predominant cellulolytic proteins and fibrolytic mechanism(s) remain elusive. Here we investigated the cellulolytic microbiome of the yak rumen by using a combination of metagenome-based and bacterial artificial chromosome (BAC)-based functional screening approaches. Totally 223 fibrolytic BAC clones were pyrosequenced and 10,070 ORFs were identified. Among them 150 were annotated as the glycoside hydrolase (GH) genes for fibrolytic proteins, and the majority (69%) of them were clustered or linked with genes encoding related functions. Among the 35 fibrolytic contigs of >10 Kb in length, 25 were derived from Bacteroidetes and four from Firmicutes. Coverage analysis indicated that the fibrolytic genes on most Bacteroidetes-contigs were abundantly represented in the metagenomic sequences, and they were frequently linked with genes encoding SusC/SusD-type outer-membrane proteins. GH5, GH9, and GH10 cellulase/hemicellulase genes were predominant, but no GH48 exocellulase gene was found. Most (85%) of the cellulase and hemicellulase proteins possessed a signal peptide; only a few carried carbohydrate-binding modules, and no cellulosomal domains were detected. These findings suggest that the SucC/SucD-involving mechanism, instead of one based on cellulosomes or the free-enzyme system, serves a major role in lignocellulose degradation in yak rumen. Genes encoding an endoglucanase of a novel GH5 subfamily occurred frequently in the metagenome, and the recombinant proteins encoded by the genes displayed moderate Avicelase in addition to endoglucanase activities, suggesting their important contribution to lignocellulose degradation in the exocellulase-scarce rumen. PMID:22808161

Examining the Impact of Smoke on Frontal Clouds and Precipitation During the 2002 Yakutsk Wildfires Using the WRF-Chem-SMOKE Model and Satellite Data

NASA Astrophysics Data System (ADS)

Lu, Zheng; Sokolik, Irina N.

2017-12-01

In 2002, an enormous amount of smoke has been emitted from Yakutsk wildfires. In this study, we examine the impact of smoke on cloud properties and precipitation associated with frontal systems using the WRF-Chem-SMOKE model and satellite data. The smoke emissions are computed using the fire radiative power technique. Smoke particles are represented as an internal mixture of organic matter (OM), black carbon (BC), and other inorganic matter, and their microphysical and radiative effects are explicitly modeled. After examining the fire activities, we identified two fire periods (FP1 and FP2). During FP1, in the cloud deck with the high cloud droplet number concentration (CDNC), but the relatively small amount of ice nuclei (IN), the rain and snow water contents (RWC and SWC) were strongly reduced, because of suppressed collision-coalescence and riming processes. The cloud cells acquired the longer lifetime and traveled farther downwind. During FP2, in the cloud deck with relatively high CDNC and IN, RWC was reduced; however, the large amounts of IN triggered the glaciation indirect effect and leaded to increased SWC. Due to the competing effects of CDNC and IN, changes in the cloud lifetime were small. Consequently, smoke-induced changes in the total cloudiness cause a dipole feature. After the smoke was nearly consumed during FP1, the large-scale dynamics of the frontal system was altered by smoke. The onset of the precipitation was delayed by 1 day. In FP2, the onset of the precipitation was not delayed but occurred at different locations, and the area-averaged precipitation was slightly reduced ( 0.5 mm/day).

Greenhouse gas aerosols and pollutants over Siberia: the YAK-AEROSIB French Russian Joint International Laboratory

NASA Astrophysics Data System (ADS)

Paris, Jean-Daniel; Belan, Boris D.; Ancellet, Gérard; Nédélec, Philippe; Arshinov, Mikhail Yu.; Pruvost, Arnaud; Berchet, Antoine; Arzoumanian, Emmanuel; Pison, Isabelle; Ciais, Philippe; Law, Kathy

2014-05-01

Despite the unique scientific value of better knowing atmospheric composition over Siberia, regional observations of the tropospheric composition over this region are still lacking. Large local anthropogenic emissions, strong ecosystem gas exchange across the vast forest expanse, and processes feeding back to global climate such as wetlands CH4 emissions, seabed hydrates destabilization and degrading permafrost make this region particularly crucial to investigate. We aim at addressing this need in the YAK-AEROSIB program by collecting high-precision in-situ measurements of the vertical distribution of CO2, CH4, CO, O3, black carbon and ultrafine particles distribution in the Siberian troposphere, as well as other parameters including aerosol lidar profiles, on a pan-Siberian aircraft transect. Campaigns are performed almost annually since 2006 until now on this regular route, while special campaigns are occasionnally arranged to sample the troposphere elsewere (e.g. Russian Arctic coast). We show the background tropospheric composition obtained from these surveys, the variability and the impact of large-scale transport of anthropogenic emissions from Europe and Asia, as well as the impact of biomass burning plumes both from local wildfires (2012) and from remote sources elsewhere in Asia. Long range transport of anthropogenic emissions is shown to have a discernible impact on O3 distribution, although its lower-tropospheric variability is largely driven by surface deposition. Regional sources and sinks drive the lower troposphere CO2 and CH4 concentrations. Recent efforts aim at better understanding the respective role of CH4 emission processes (including methanogenesis in wetlands and emissions by wildfires) in driving its large scale atmospheric variability over the region. Generally, the YAK AEROSIB provide unique observations over Siberia, documenting both direct impact of regional sources and aged air masses experiencing long range transport toward the high Arctic.

Total suspended particulate matter and toxic elements indoors during cooking with yak dung

NASA Astrophysics Data System (ADS)

Kang, Shichang; Li, Chaoliu; Wang, Feiyue; Zhang, Qianggong; Cong, Zhiyuan

Many herders in the Tibetan Plateau still inherit the traditional lifestyle, including living in tents and burning yak dung for fuel. This short correspondence reports a pilot study on indoor air quality in the nomadic tents in the Nam Co region, inland Tibetan Plateau. The results showed very high concentrations of total suspended particles (TSP), averaging at 4.45 mg m -3 during the cooking/heating period (with daily value of 3.16 mg m -3). Elevated concentrations of toxic element Cd, As and Pb were also found within the tents, averaging 3.16 μg m -3, 35.00 μg m -3, and 81.39 μg m -3 for a day, respectively, which were not only far higher than those of WHO indoor air quality guidelines, but also more than 10 4-10 6 times higher than the outdoor air level in the Nam Co area. The study raises serious concerns over the health of Tibetan herders following their long-term exposure to the tent air.

Organochlorine pesticides and polychlorinated biphenyls in air, grass and yak butter from Namco in the central Tibetan Plateau.

PubMed

Wang, Chuanfei; Wang, Xiaoping; Yuan, Xiaohua; Ren, Jiao; Gong, Ping

2015-06-01

Limited studies on bioaccumulation of persistent organic pollutants (POPs) along terrestrial food chains were conducted. The food chain air-grass-yak (butter) in the pasture region of Namco in the central Tibetan Plateau (TP) was chosen for study. The air, grass and butter POPs in the TP were at the lower end of the concentrations generally found around the globe. HCB was the main pollutant in air and butter. Besides HCB, β-HCH and p,p'-DDE were the other major compounds in butter. Along the food chain, DDTs and high molecular weight PCB-138, 153 and 180 had higher Biological Concentration Factor values. The air-butter transfer factors of POPs were derived and demonstrated the practical advantage in predicting the atmospheric OCPs and PCBs to the TP. This study sheds light on the transfer and accumulation of POPs along the terrestrial food chain of the TP. Copyright © 2015 Elsevier Ltd. All rights reserved.

Left hand polydactyly: a case report.

PubMed

Mumoli, Nicola; Gandini, Daniele; Wamala, Edris Kalanzi; Cei, Marco

2008-11-24

Polydactyly is a congenital anomaly with a wide range of manifestations that occurs in many forms, ranging from varying degrees of mere splitting to completely duplicated thumb. When duplication occurs alone, it is usually unilateral and sporadic. In this case report we describe an otherwise healthy 19-year-old woman of Tibetan heritage with isolated left hand preaxial polydactyly. She experienced working related difficulties in her daily yak's milking. She subsequently underwent surgical correction, and the over number thumb was removed with associated meticulous skeletal and soft tissue reconstruction. Polydactyly is the most common congenital digital anomaly of the hand and foot. It can occur in isolation or as part of a syndrome. Surgery is necessary to create a single, functioning thumb and is indicated to improve cosmesis. Skin, nail, bone, ligament, and musculoskeletal elements must be combined to reconstruct an optimal digit. In this case (Tibetan society is almost exclusively a sheep-breeding one) surgery was necessary to leave a single, functioning thumb for her work as yak milkmaid.

Mass content of ultrahigh-energy cosmic rays within different time periods

NASA Astrophysics Data System (ADS)

Glushkov, A. V.

2014-03-01

Estimates obtained for the average atomic number of nuclei of primary particles with energies in the region of E 0 ⩾ 1015 eV over the past 36 years at the Yakutsk array and other arrays worldwide for studying extensive air showers are presented. It is shown that these estimates are markedly different with in different time periods. Earlier than 1996, the composition of cosmic rays in the energy range of 5 × 1015-1018 eV was markedly lighter than in later years. After 2008, there appeared a trend toward a decrease in . This is likely to be a manifestation of some explosive process in the Milky Way Galaxy after 1996.

7 CFR 1430.102 - Eligible products.

Code of Federal Regulations, 2013 CFR

2013-01-01

... must be manufactured from dairy cow's milk produced in the United States, and must not have been previously owned by CCC. Dairy cow in this instance means an animal of the kind that produces the majority of dairy products in the United States and not, for example, cows of other species of animals such as yaks...

7 CFR 1430.102 - Eligible products.

Code of Federal Regulations, 2011 CFR

2011-01-01

... must be manufactured from dairy cow's milk produced in the United States, and must not have been previously owned by CCC. Dairy cow in this instance means an animal of the kind that produces the majority of dairy products in the United States and not, for example, cows of other species of animals such as yaks...

7 CFR 1430.102 - Eligible products.

Code of Federal Regulations, 2012 CFR

2012-01-01

... must be manufactured from dairy cow's milk produced in the United States, and must not have been previously owned by CCC. Dairy cow in this instance means an animal of the kind that produces the majority of dairy products in the United States and not, for example, cows of other species of animals such as yaks...

7 CFR 1430.102 - Eligible products.

Code of Federal Regulations, 2014 CFR

2014-01-01

... must be manufactured from dairy cow's milk produced in the United States, and must not have been previously owned by CCC. Dairy cow in this instance means an animal of the kind that produces the majority of dairy products in the United States and not, for example, cows of other species of animals such as yaks...

Is the 20th century warming unprecedented in the Siberian north?

NASA Astrophysics Data System (ADS)

Sidorova, Olga V.; Saurer, Matthias; Andreev, Andrei; Fritzsche, Diedrich; Opel, Thomas; Naurzbaev, Mukhtar M.; Siegwolf, Rolf

2013-08-01

To answer the question "Has the recent warming no analogues in the Siberian north?" we analyzed larch tree samples (Larix gmelinii Rupr.) from permafrost zone in the eastern Taimyr (TAY) (72°N, 102°E) using tree-ring and stable isotope analyses for the Climatic Optimum Period (COP) 4111-3806 BC and Medieval Warm Period (MWP) 917-1150 AD, in comparison to the recent period (RP) 1791-2008 AD. We developed a description of the climatic and environmental changes in the eastern Taimyr using tree-ring width and stable isotope (δ13C, δ18O) data based on statistical verification of the relationships to climatic parameters (temperature and precipitation). Additionally, we compared our new tree-ring and stable isotope data sets with earlier published July temperature and precipitation reconstructions inferred from pollen data of the Lama Lake, Taimyr Peninsula, δ18O ice core data from Akademii Nauk ice cap on Severnaya Zemlya (SZ) and δ18O ice core data from Greenland (GISP2), as well as tree-ring width and stable carbon and oxygen isotope data from northeastern Yakutia (YAK). We found that the COP in TAY was warmer and drier compared to the MWP but rather similar to the RP. Our results indicate that the MWP in TAY started earlier and was wetter than in YAK. July precipitation reconstructions obtained from pollen data of the Lama Lake, oxygen isotope data from SZ and our carbon isotopes in tree cellulose agree well and indicate wetter climate conditions during the MWP. Consistent large-scale patterns were reflected in significant links between oxygen isotope data in tree cellulose from TAY and YAK, and oxygen isotope data from SZ and GISP2 during the MWP and the RP. Finally, we showed that the recent warming is not unprecedented in the Siberian north. Similar climate conditions were recorded by tree-rings, stable isotopes, pollen, and ice core data 6000 years ago.

Methods for Scaling to Doubly Stochastic Form,

DTIC Science & Technology

1981-06-26

Frobenius -Konig Theorem (MARCUS and MINC [1964],p 97) A nonnegative n xn matrix without support contains an s x t zero subma- trix where: s +t =n + -3...that YA(k) has row sums 1. Then normalize the columns by a diagonal similarity transform defined as follows: Let x = (zx , • z,,) be a left Perron vector

7 CFR 760.204 - Eligible livestock, honeybees, and farm-raised fish.

Code of Federal Regulations, 2012 CFR

2012-01-01

... beef cows or bulls, (2) Adult buffalo or beefalo cows or bulls, (3) Adult dairy cows or bulls, (4... determined by FSA; (2) Yaks; (3) Ostriches; (4) All beef and dairy cattle, and buffalo and beefalo that... alpacas, adult or non-adult dairy cattle, beef cattle, beefalo, buffalo, deer, elk, emus, equine, goats...

7 CFR 760.204 - Eligible livestock, honeybees, and farm-raised fish.

Code of Federal Regulations, 2014 CFR

2014-01-01

... beef cows or bulls, (2) Adult buffalo or beefalo cows or bulls, (3) Adult dairy cows or bulls, (4... determined by FSA; (2) Yaks; (3) Ostriches; (4) All beef and dairy cattle, and buffalo and beefalo that... alpacas, adult or non-adult dairy cattle, beef cattle, beefalo, buffalo, deer, elk, emus, equine, goats...

7 CFR 760.204 - Eligible livestock, honeybees, and farm-raised fish.

Code of Federal Regulations, 2011 CFR

2011-01-01

... beef cows or bulls, (2) Adult buffalo or beefalo cows or bulls, (3) Adult dairy cows or bulls, (4... determined by FSA; (2) Yaks; (3) Ostriches; (4) All beef and dairy cattle, and buffalo and beefalo that... alpacas, adult or non-adult dairy cattle, beef cattle, beefalo, buffalo, deer, elk, emus, equine, goats...

7 CFR 760.204 - Eligible livestock, honeybees, and farm-raised fish.

Code of Federal Regulations, 2013 CFR

2013-01-01

... beef cows or bulls, (2) Adult buffalo or beefalo cows or bulls, (3) Adult dairy cows or bulls, (4... determined by FSA; (2) Yaks; (3) Ostriches; (4) All beef and dairy cattle, and buffalo and beefalo that... alpacas, adult or non-adult dairy cattle, beef cattle, beefalo, buffalo, deer, elk, emus, equine, goats...

The M-15-aircraft (samolot M-15)

NASA Technical Reports Server (NTRS)

Leiiecki, R.

1978-01-01

The M-15 is an all-metal, semimonocoque, twin-tail-boom sesquiplane aircraft designed exclusively for agricultural support operations involving slow low-level flight. It is powered by a single Al-25 bypass turbojet engine used in the Yak-40 aircraft. Tanks for spraying chemicals are mounted between the lower and upper wings. Dimensions, weights, and performance data are tabulated.

College Students' Experiences with Anonymous Social Media: Implications for Campus Racial Climate

ERIC Educational Resources Information Center

Armstrong, Amanda; Thomas, Jaymi; Smith, Madeline

2017-01-01

The purpose of this exploratory case study was to gather undergraduate students' perceptions of anonymous racist messages found on Yik Yak and to better understand the implications of anonymous platforms on campus racial climate. Given the limited research surrounding students' use of anonymous social media platforms, as well as the predominant…

Social Media and Higher Education: The Problem of Anonymous Electronic Threats to the Campus Community

ERIC Educational Resources Information Center

Reynolds, Cristin Lee; Platt, R. Eric; Malone Schaffer, Lenore; Foster, Holly

2017-01-01

This case is for use in graduate courses pertaining to student affairs and higher education administration. It presents challenges higher education professionals face concerning anonymous social media, and specifically how threats made through anonymous social media platforms such as Yik Yak affect the entire university community. The anonymity of…

Challenging the New World Order: The Arms Transfer Policies of the Russian Republic

DTIC Science & Technology

1993-10-01

SU-22 fighter, SU-24 and SU-25 ground attack planes, MiG-29, MiG-31 fighters, 11-76 transports, " secondhand " AN-24 and Yak-40 passenger aircraft, i.e...notably in the Third World, and is the author of a forthcoming study of the Soviet Commissariat of Nationalities and editor of books on Soviet

Logical account of a terminological tool

NASA Astrophysics Data System (ADS)

Bresciani, Paolo

1991-03-01

YAK (Yet Another Krapfen) is a hybrid knowledge representation environment following the tradition of KL-ONE and KRYPTON. In its terminological box (TBOX) concepts and roles are described by means of a language called KFL that, even if inspired to FL-, captures a different set of descriptions, especially allowing the formulation of structured roles, that are aimed to be more adequate for the representational goal. KFL results to have a valid and complete calculus for the notion of subsumption, and a tractable algorithm that realize it is available. In the present paper it is shown how the semantics of a sufficiently significative subset of KFL can be described in terms of standard first order logic semantics. It is so possible to develop a notion of 'relation' between KFL and a full first order logic language formulated ad hoc and usable as formalization of an assertional box (ABOX). We then use this notion to justify the use of the terminological classification algorithm of the TBOX of YAK as part of the deduction machinery of the ABOX. In this way, the whole hybrid environment can take real and consistent advantages both from the TBOX and its tractable classification algorithm, and from the ABOX, and its wider expressive capability.

Personal PM2.5 and indoor CO in nomadic tents using open and chimney biomass stoves on the Tibetan Plateau

NASA Astrophysics Data System (ADS)

Li, Chaoliu; Kang, Shichang; Chen, Pengfei; Zhang, Qianggong; Guo, Junming; Mi, Jue; Basang, Puchi; Luosang, Quzhen; Smith, Kirk R.

2012-11-01

Yak dung is the primary source of energy for cooking and heating of nomadic Tibetan herders. Personal PM2.5 and indoor CO concentrations and time-activity patterns were investigated in nomadic tents with open stoves and locally available chimney stoves. Personal PM2.5 monitoring using a light-scattering datalogger was performed with women in five tents with open fires and four with chimney stoves over 3 days. Meanwhile, indoor CO variation was also measured. Results showed that 24 h average concentrations of PM2.5 and CO in the tents with open stoves were 1.42 mg m-3 (n = 5, SD = 3.26) and 6.69 mg m-3 (n = 4; SD = 9.11), respectively, which were significantly higher than the tents with chimney stoves having 0.14 mg m-3 (n = 4; SD = 0.65) and 0.12 mg m-3 (n = 4; SD = 1.01) of PM2.5 and CO, respectively. Although chimney stoves significantly reduced indoor air pollution, the concentration of PM2.5 was still higher than annual WHO Air Quality Guideline (0.035 mg m-3). Diurnal variability of PM2.5 and CO was similar and had multiple peaks. This phenomenon was closely connected with behaviors of the participants within the tents. Average 1-h peak concentrations of PM2.5 and CO exceed 24-h mean values by a factor of 5.0 and 4.3, respectively. Significant correlation between hourly PM2.5 and CO concentrations was revealed. Generally, women and children spent 7 h longer than other family members within the tent each day and were thus exposed to higher levels of pollutants. Secondhand tobacco smoke and burning of yak oil lamps are also present in many households, but are much smaller contributors to the exposures. Therefore, yak dung combustion contributes substantially to the personal exposure of householders in this setting even during the warmest time of year in this setting and that although exposures are greatly reduced with chimney stoves; they are still high by comparison to national standards or WHO guidelines.

Yak dung combustion aerosols in the Tibetan Plateau: Chemical characteristics and influence on the local atmospheric environment

NASA Astrophysics Data System (ADS)

Chen, Pengfei; Kang, Shichang; Bai, Jiankun; Sillanpää, Mika; Li, Chaoliu

2015-04-01

The study of the source areas of atmospheric pollutants in the Tibetan Plateau (TP) - one of the most remote regions in the world - has raised a great deal of concern. It is generally considered that the majority of pollutants in this region are transported from outside the TP. This research investigated the water soluble elements and carbonaceous matter from aerosols emitted from yak dung combustion by local residents and re-analyzed previous OC and BC data at Nam Co - a remote area of the TP. The compositions of the water soluble elements of the studied aerosols were similar to those in precipitation and snow samples of the region under investigation. Some heavy metal elements (e.g. Cd and As) even had higher enrichment factor (EF) values (1793 and 2355, respectively) compared to those in precipitation and snow samples, implying that previously reported high EF values for precipitation and snow did not completely reflect the long-range transported pollutants from outside the TP. Accordingly, the contributions of local sources needed to be considered. Organic carbon (OC) and black carbon (BC) accounted for 55.2% and 3.63% of the studied aerosol, respectively. The OC/BC ratio of the studied aerosols was close to the corresponding value for the outdoor aerosols, further indicating the influence of local sources on the atmosphere of Nam Co. It was proposed that air masses from South Asia cause high BC concentrations in the Nam Co region. It was, however, discovered that air masses from the TP itself also induce high BC concentrations, suggesting that not all the BC of Nam Co was transported from South Asia. Therefore, it is proposed that pollutants of atmospheric aerosols of the Nam Co region were derived from a variety of sources from both the TP and outside. In other words, the influence of yak dung burning by local residents on the atmosphere of the TP cannot be overlooked. Correspondingly, long-range transported pollutants can penetrate into the inland TP only when intensified pollution events occur in South Asia.

Continental Evolution Involving Subduction Underplating and Synchronous Foreland Thrusting: Evidence from the Trans-Alaska Crustal Transect

NASA Astrophysics Data System (ADS)

Fuis, G. S.; Moore, T. E.; Plafker, G.; Brocher, T. M.; Fisher, M. A.; Mooney, W. D.; Nokleberg, W. J.; Page, R. A.; Beaudoin, B. C.; Christensen, N. I.; Levander, A.; Lutter, W. J.; Saltus, R. W.; Ruppert, N. A.

2010-12-01

We investigated the crustal structure and tectonic evolution of the North American continent in Alaska, where the continent has grown through magmatism, accretion, and tectonic underplating. In the 1980’s and early 1990’s, we conducted a geological and geophysical investigation, known as the Trans-Alaska Crustal Transect (TACT), along a 1350-km-long corridor from the Aleutian Trench to the Arctic coast. The most distinctive crustal structures and the deepest Moho along the transect are located near the Pacific and Arctic margins. Near the Pacific margin, we infer a stack of tectonically underplated oceanic layers interpreted to be remnants of the extinct Kula (or Resurrection) Plate. Continental Moho just north of this underplated stack is more than 55 km deep. Near the Arctic margin, the Brooks Range is underlain by north-vergent, crustal-scale duplexes that overlie a ramp on autochthonous North Slope crust. There, Moho has been depressed to nearly 50-km depth. In contrast, the Moho of central Alaska is on average 32 km deep. In the Paleogene, tectonic underplating of Kula- (or Resurrection-) Plate fragments overlapped in time with duplexing in the Brooks Range. Possible tectonic models linking these two widely separated regions include “flat-slab” subduction and an “orogenic-float” model. In the Neogene, the collision of the Yakutat terrane (YAK), in southern Alaska, correlates with renewed compression in northeast Alaska and northwest Canada, in a fashion somewhat similar to the tectonics in the Paleogene. The Yakutat terrane, riding atop the subducting Pacific oceanic lithosphere (POL), spans a newly interpreted tear in the POL. East of the tear, POL is interpreted to subduct steeply and alone beneath the Wrangell arc volcanoes because the overlying YAK has been left behind as tectonically underplated rocks beneath the rising St. Elias Range in the coastal region. West of the tear, the YAK and POL are interpreted to subduct together at a gentle angle (a few degrees from 0 to 400 km from the trench), and this thickened package inhibits arc volcanism.

Airborne lead and other elements derived from local fires in the Himalayas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Davidson, C.I.; Grimm, T.C.; Nasta, M.A.

1981-12-18

The combustion of wood and yak dung for heating and cooking in a populated Nepal Himalayan valley contributes significantly to the ambient airborne concentrations of lead, copper, aluminum, magnesium, and elemental and organic carbon. A comparison of the concentrations of these elements in fresh snow with corresponding values in air suggests that the pollution aerosol is confined to the valley, with pristine air aloft.

Genetic variation in N- and C-terminal regions of bovine DNAJA1 heat shock protein gene in African, Asian and American cattle.

PubMed

Ajayi, Oyeyemi O; Peters, Sunday O; De Donato, Marcos; Mujibi, F Denis; Khan, Waqas A; Hussain, Tanveer; Babar, Masroor E; Imumorin, Ikhide G; Thomas, Bolaji N

2018-01-01

DNAJA1 or heat shock protein 40 (Hsp40) is associated with heat adaptation in various organisms. We amplified and sequenced a total of 1,142 bp of bovine Hsp40 gene representing the critical N-terminal (NTR) and C-terminal (CTR) regions in representative samples of African, Asian and American cattle breeds. Eleven and 9 different haplotypes were observed in the NTR in Asian and African breeds respectively while in American Brangus, only two mutations were observed resulting in two haplotypes. The CTR appears to be highly conserved between cattle and yak. In-silico functional analysis with PANTHER predicted putative deleterious functional impact of c.161 T>A; p. V54Q while alignment of bovine and human NTR-J domains revealed that p.Q19H, p.E20Q and p. E21X mutations occurred in helix 2 and p.V54Q missense mutation occurred in helix 3 respectively. The 124 bp insertion found in the yak DNAJA1 ortholog may have significant functional relevance warranting further investigation. Our results suggest that these genetic differences may be concomitant with population genetic history and possible functional consequences for climate adaptation in bovidae.

Metabolomic biomarkers identify differences in milk produced by Holstein cows and other minor dairy animals.

PubMed

Yang, Yongxin; Zheng, Nan; Zhao, Xiaowei; Zhang, Yangdong; Han, Rongwei; Yang, Jinhui; Zhao, Shengguo; Li, Songli; Guo, Tongjun; Zang, Changjiang; Wang, Jiaqi

2016-03-16

Several milk metabolites are associated with breeds or species of dairy animals. A better understanding of milk metabolites from different dairy animals would advance their use in evaluating milk traits and detecting milk adulteration. The objective of this study was to characterize the milk metabolite profiles of Chinese Holstein, Jersey, yak, buffalo, goat, camel, and horse and identify any differences using non-targeted metabolomic approaches. Milk samples were tested using nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography-tandem mass spectrometry (LC-MS). Data were analyzed using a multivariate analysis of variance and differences in milk metabolites between Holstein and the other dairy animals were assessed using orthogonal partial least-squares discriminant analysis. Differential metabolites were identified and some metabolites, such as choline and succinic acid, were used to distinguish Holstein milk from that of the other studied animals. Metabolic pathway analysis of different metabolites revealed that glycerophospholipid metabolism as well as valine, leucine, and isoleucine biosynthesis were shared in the other ruminant animals (Jersey, buffalo, yak, and goat), and biosynthesis of unsaturated fatty acids was shared in the non-ruminant animals (camel and horse). These results can be useful for gaining a better understanding of the differences in milk synthesis between Holstein and the other dairy animals. Copyright © 2016. Published by Elsevier B.V.

Toward understanding the role of the atmosphere in pan Arctic change and sea ice loss; an update on the status of focused campaigns under POLARCAT.

NASA Astrophysics Data System (ADS)

Burkhart, J. F.; Bates, T.; Brock, C. A.; Clerbaux, C.; Crawford, J. H.; Dibb, J. E.; Elansky, N.; Ghan, S.; Hirdman, D.; Honrath, R. E.; Jacob, D. J.; Law, K.; Paris, J.; Quinn, P.; Schlager, H.; Singh, H. B.; Sodemann, H.; Stohl, A.

2008-12-01

Sea ice loss reached an extraordinary extent in 2007, decreasing in area more than 2.5 million square kilometres below the 1979 extent. Sea ice loss is one of many Arctic processes resulting from a warming climate. The dynamics of a changing Arctic system are particularly sensitive to climate change and filled with uncertainties and complex feedback mechanisms - most being simply unknown. During the International Polar Year (IPY) a number of international partnerships were formed to establish the Polar Study using Aircraft, Remote Sensing, Surface Measurements and Models, of Climate, Chemistry, Aerosols, and Transport (POLARCAT). Under the umbrella of POLARCAT projects cooperated with national funding to undertake the most comprehensive assessment of air pollution impacts on the Arctic to date. In the spring and summer of 2008 more than 20 institutes from ten nations participated in intensive aircraft, ship, and station-based campaigns with accompanying efforts from the satellite and modelling communities to provide near real time products for mission planning and analysis. The campaigns provided an assessment of the role that tropospheric chemistry, aerosols, and transport play in the Arctic. The spring campaigns focused on anthropogenic pollution, while the summer campaigns targeted biomass burning. During the spring of 2008, over 80 flights were flown by five different aircraft as part of the ARCTAS, ISDAC, ARCPAC, and French POLARCAT campaigns, the ICEALOT campaign commissioned the R/V Knorr to travel over 12,000 km, and numerous specialty satellite and modelling products were developed with near real time distribution. These same products were again used for flight planning and forecasting in the summer when an additional 50+ flights were flown by the ARCTAS, French POLARCAT, Siberian YAK, and GRACE campaigns. Several ground based stations and the Siberian TROICA campaign also conducted intensive operating periods (IOPs). We present an overview of the individual campaigns, anticipated products, and initial "quicklooks" from these activities.

The cosmic ray spectrum above 10(19) EV at Volcano Ranch and Haverah Park

NASA Technical Reports Server (NTRS)

Linsley, J.

1986-01-01

The cosmic ray energy per particle spectrum above 10 to the 19th power eV is measured the same way that energy spectra are measured at much lower energies, by counting all of the particles in a specified energy range that are incident per unit time with trajectories within specified geometrical limits. Difficulties with background or poorly known detection efficiency are markedly less than in some other cosmic ray measurements. The fraction of primary energy given to muons, neutrinos, and slow hadrons is less than 10% in this region, so the primary energy equals the track length integral of the secondary electrons with only a small correction for the energy given to other kinds of particles. Results from Volcano Ranch and Haverah Park are compared with results from the Yakutsk experiment.

Energy estimation of inclined air showers with help of detector responses

NASA Astrophysics Data System (ADS)

Dedenko, L. G.; Fedorova, G. F.; Fedunin, E. Yu.; Glushkov, A. V.; Kolosov, V. A.; Podgrudkov, D. A.; Pravdin, M. I.; Roganova, T. M.; Sleptsov, I. E.

2004-11-01

The method of groups of muons have been suggested to estimate the detector responses for the inclined giant air shower in terms of quark-gluon string model with the geomagnetic field taken into account. Groups are average numbers of muons of positive or negative sign in small intervals of energy, height production and direction of motion in the atmosphere estimated with help of transport equations. For every group a relativistic equation of motion has been solved with geomagnetic field and ionization losses taken into account. The response of a detector and arrival time for every group which strike a detector has been estimated. The energy of the inclined giant air shower estimated with help of calculated responses and the data observed at the Yakutsk array happens to be above 10 20 eV.

Reactor Application for Coaching Newbies

DOE Office of Scientific and Technical Information (OSTI.GOV)

2015-06-17

RACCOON is a Moose based reactor physics application designed to engage undergraduate and first-year graduate students. The code contains capabilities to solve the multi group Neutron Diffusion equation in eigenvalue and fixed source form and will soon have a provision to provide simple thermal feedback. These capabilities are sufficient to solve example problems found in Duderstadt & Hamilton (the typical textbook of senior level reactor physics classes). RACCOON does not contain any advanced capabilities as found in YAK.

Magnetospheric Effects during the Approach of the Chelyabinsk Meteoroid

NASA Astrophysics Data System (ADS)

Chernogor, L. F.

2018-03-01

We have analyzed the observational results for variations in the main geomagnetic field and its fluctuations in the range of periods 1-1000 s that accompanied the approach of the Chelyabinsk space body to the magnetosphere and ionosphere of the Earth. The measurements were conducted with a magnetometerfluxmeter near the city of Kharkiv, as well as with the network of magnetometers located at the observatories of Novosibirsk, Kyiv, Lviv, Almaty, Khabarovsk, Arti, Borok, and Yakutsk. Variations in the main geomagnetic field and its fluctuations approximately 33-47 min prior to the explosion of the Chelyabinsk meteoroid have been discovered; they persisted for 25-35 min and were probably associated with meteoroid passage through the magnetosphere. The amplitude of variations reached 1-6 nT. We have proposed a model of the generation of aperiodic, quasi-periodic, and noise-like variations in the geomagnetic field induced by the approach of a space body.

Effect of strategic feed supplementation on productive and reproductive performance in yak cows.

PubMed

Long, R J; Zhang, D G; Wang, X; Hu, Z Z; Dong, S K

1999-01-27

A total of 2230 yak cows (5-13 years of age) in two populations with different milking systems were investigated. One population had a system of milking once a day (MOD), and the other population twice a day (MTD). The average milk yield of MOD cows was 0.7 +/- 0.2 kg/day within a milking period of 109 +/- 9 days. This compared with an average of 1.24 +/- 0.3 kg/day in 127 +/- 6 days in MTD yaks (p < 0.01). The cows showed a calving rate of 71% under the MOD system and 51.4% under the MTD system. Three farms with a total of 104 MTD cows between six and 12 years of age were used to provide three different feeding groups. The groups were fed with, or without oat hay or highland barley straw in amounts of 1-1.5 kg/head/day from December to April. The three farms were designated as Farm I, Farm II, and Farm III. Farm I had 41 cows with body weight of 230 +/- 67 kg each for grazing with no supplement (GNS). Farm II had 30 animals with body weight of 216 +/- 28 kg each for grazing + oat hay (GOH). Farm III had 33 animals body weight of 221 +/- 34 kg each for grazing + highland barely straw (GBS). The calving rates of the cows in GOH and GBS were 23 and 19% higher, respectively, than GNS cows (p < 0.01), and the highest rate reached 76.7% in GOH. The live weight loss of the cows in GNS was considerably higher (p < 0.01) than in the two other groups. Ten GOH cows and 12 GNS cows were used to collect milk samples for measuring the progesterone concentration using RIA kits provided by IAEA/FAO: Milk was sampled every five days from calving until 90 days postpartum. In the unsupplemented group, milk progesterone (P4) levels suggested that cows had started cyclic ovarian activity by 40 days postpartum, whereas only 25% had been observed in estrus. In the supplemented group, 80% of cows had started cyclic ovarian activity by the same time and 70% had been seen in estrus. Two types of cyclic activity in terms of progesterone changed were found. With Type I (normal), 50 and 80% of cows from GNS and GOH, respectively, had cyclic changes of P4 in milk at 40 days postpartum. With Type II, the P4 levels in the milk remained 0.89 ng/ml until 90 days postpartum. A total of 46 grazing cows between five and 13 years of age (body weight 214 +/- 68 kg) was used to collect blood samples to measure concentrations of nutrient of metabolites at two weeks pre-calving and at two weeks, two months and four months postcalving, respectively. The concentrations of nutrient metabolites [albumin, globulin, urea, beta-hydroxybutyrate (BHB) and inorganic phosphorus] suggested general underfeeding of energy and protein in the winter/late pregnancy period with some recovery in lactation. Energy constraints appeared again as the summer progressed. No dietary phosphate deficiency was found. BHB and albumin testing on serum yaks could be a useful tool to identify poor nutritional status during the winter and so illustrate the need for supplementation.

Impacts of dung combustion on the carbon cycle of alpine grassland of the north Tibetan plateau.

PubMed

Xu, Zengrang; Cheng, Shengkui; Zhen, Lin; Pan, Ying; Zhang, Xianzhou; Wu, Junxi; Zou, Xiuping; Bijaya, G C Dhruba

2013-08-01

Alpine grassland of Tibet is a frangible ecosystem in terms of carbon (C) emission. Yak dung is an important resident energy with about 80 % of yak dung combusted for energy in the north Tibetan plateau. This paper investigated the impact of dung combustion on the C cycle of the alpine grassland ecosystem in north Tibet, China. During the growing season of 2011, from a field survey and household questionnaires, the main impacts of dung collection for fuel on the C cycle of the ecosystem were identified. (1) The C sequestration and storage capacity, including the dung-derived C stored in soil and C captured by vegetation, decreased. The net primary production decreased remarkably because of the reduction of dung returned to soil. (2) In a given period, more C was emitted to the atmosphere in the dung combustion situation than that in the dung returned to soil situation. (3) The energy grazing alpine meadow ecosystem changed into a net C source, and the net biome production of the ecosystem dropped to -15.18 g C/m2 year in the dung combustion situation, 42.95 g C/m2 year less than that in the dung returned situation. To reduce the CO2 emission derived from dung use, the proportion of dung combustion should be reduced and alternative renewable energy such as solar, wind, or hydro energy should be advocated, which is suitable for, and accessible to, the north Tibetan plateau.

Impacts of Dung Combustion on the Carbon Cycle of Alpine Grassland of the North Tibetan Plateau

NASA Astrophysics Data System (ADS)

Xu, Zengrang; Cheng, Shengkui; Zhen, Lin; Pan, Ying; Zhang, Xianzhou; Wu, Junxi; Zou, Xiuping; Bijaya, G. C. Dhruba

2013-08-01

Alpine grassland of Tibet is a frangible ecosystem in terms of carbon (C) emission. Yak dung is an important resident energy with about 80 % of yak dung combusted for energy in the north Tibetan plateau. This paper investigated the impact of dung combustion on the C cycle of the alpine grassland ecosystem in north Tibet, China. During the growing season of 2011, from a field survey and household questionnaires, the main impacts of dung collection for fuel on the C cycle of the ecosystem were identified. (1) The C sequestration and storage capacity, including the dung-derived C stored in soil and C captured by vegetation, decreased. The net primary production decreased remarkably because of the reduction of dung returned to soil. (2) In a given period, more C was emitted to the atmosphere in the dung combustion situation than that in the dung returned to soil situation. (3) The energy grazing alpine meadow ecosystem changed into a net C source, and the net biome production of the ecosystem dropped to -15.18 g C/m2 year in the dung combustion situation, 42.95 g C/m2 year less than that in the dung returned situation. To reduce the CO2 emission derived from dung use, the proportion of dung combustion should be reduced and alternative renewable energy such as solar, wind, or hydro energy should be advocated, which is suitable for, and accessible to, the north Tibetan plateau.

Phylogeny of anaerobic fungi (phylum Neocallimastigomycota), with contributions from yak in China.

PubMed

Wang, Xuewei; Liu, Xingzhong; Groenewald, Johannes Z

2017-01-01

The phylum Neocallimastigomycota contains eight genera (about 20 species) of strictly anaerobic fungi. The evolutionary relationships of these genera are uncertain due to insufficient sequence data to infer their phylogenies. Based on morphology and molecular phylogeny, thirteen isolates obtained from yak faeces and rumen digesta in China were assigned to Neocallimastix frontalis (nine isolates), Orpinomyces joyonii (two isolates) and Caecomyces sp. (two isolates), respectively. The phylogenetic relationships of the eight genera were evaluated using complete ITS and partial LSU sequences, compared to the ITS1 region which has been widely used in this phylum in the past. Five monophyletic lineages corresponding to six of the eight genera were statistically supported. Isolates of Caecomyces and Cyllamyces were present in a single lineage and could not be separated properly. Members of Neocallimastigomycota with uniflagellate zoospores represented by Piromyces were polyphyletic. The Piromyces-like genus Oontomyces was consistently closely related to the traditional Anaeromyces, and separated the latter genus into two clades. The phylogenetic position of the Piromyces-like genus Buwchfawromyces remained unresolved. Orpinomyces and Neocallimastix, sharing polyflagellate zoospores, were supported as sister genera in the LSU phylogeny. Apparently ITS, specifically ITS1 alone, is not a good marker to resolve the generic affinities of the studied fungi. The LSU sequences are easier to align and appear to work well to resolve generic relationships. This study provides a comparative phylogenetic revision of Neocallimastigomycota isolates known from culture and sequence data.

Convergent Evolution of Rumen Microbiomes in High-Altitude Mammals.

PubMed

Zhang, Zhigang; Xu, Dongming; Wang, Li; Hao, Junjun; Wang, Jinfeng; Zhou, Xin; Wang, Weiwei; Qiu, Qiang; Huang, Xiaodan; Zhou, Jianwei; Long, Ruijun; Zhao, Fangqing; Shi, Peng

2016-07-25

Studies of genetic adaptation, a central focus of evolutionary biology, most often focus on the host's genome and only rarely on its co-evolved microbiome. The Qinghai-Tibetan Plateau (QTP) offers one of the most extreme environments for the survival of human and other mammalian species. Yaks (Bos grunniens) and Tibetan sheep (T-sheep) (Ovis aries) have adaptations for living in this harsh high-altitude environment, where nomadic Tibetan people keep them primarily for food and livelihood [1]. Adaptive evolution affects energy-metabolism-related genes in a way that helps these ruminants live at high altitude [2, 3]. Herein, we report convergent evolution of rumen microbiomes for energy harvesting persistence in two typical high-altitude ruminants, yaks and T-sheep. Both ruminants yield significantly lower levels of methane and higher yields of volatile fatty acids (VFAs) than their low-altitude relatives, cattle (Bos taurus) and ordinary sheep (Ovis aries). Ultra-deep metagenomic sequencing reveals significant enrichment in VFA-yielding pathways of rumen microbial genes in high-altitude ruminants, whereas methanogenesis pathways show enrichment in the cattle metagenome. Analyses of RNA transcriptomes reveal significant upregulation in 36 genes associated with VFA transport and absorption in the ruminal epithelium of high-altitude ruminants. Our study provides novel insights into the contributions of microbiomes to adaptive evolution in mammals and sheds light on the biological control of greenhouse gas emissions from livestock enteric fermentation. Copyright © 2016 Elsevier Ltd. All rights reserved.

A Business Overview & Summary of the SM-27S/T MACHETE RDT&E Program as Undertaken by the Military Aerospace/Tactical Air Warfare Systems Division of STAVATTI

DTIC Science & Technology

2005-06-28

Malaysia , Syria and Saudi Arabia. As a matter of fact, Iraqi resistance fighters today are known throughout the nation as the Mojahedin. The vast...Karakorum, PZL I-22 Iryda, Samsung -Lockheed KTX-2, and Yakolev/Aermachi Yak-130, all of these aircraft are high performance, subsonic jet trainers which...annually. These assembly workers may be unionized, however, it is likely that STAVATTI wages, employee benefit packages and mature treatment of all workforce

Carnivore-caused livestock mortality in Trans-Himalaya.

PubMed

Namgail, Tsewang; Fox, Joseph L; Bhatnagar, Yash Veer

2007-04-01

The loss of livestock to wild predators is an important livelihood concern among Trans-Himalayan pastoralists. Because of the remoteness and inaccessibility of the region, few studies have been carried out to quantify livestock depredation by wild predators. In the present study, we assessed the intensity of livestock depredation by snow leopard Uncia uncia, Tibetan wolf Canis lupus chanku, and Eurasian lynx Lynx l. isabellina in three villages, namely Gya, Rumtse, and Sasoma, within the proposed Gya-Miru Wildlife Sanctuary in Ladakh, India. The three villages reported losses of 295 animals to these carnivores during a period of 2.5 years ending in early 2003, which represents an annual loss rate of 2.9% of their livestock holdings. The Tibetan wolf was the most important predator, accounting for 60% of the total livestock loss because of predation, followed by snow leopard (38%) and lynx (2%). Domestic goat was the major victim (32%), followed by sheep (30%), yak (15%), and horse (13%). Wolves killed horses significantly more and goats less than would be expected from their relative abundance. Snow leopards also killed horses significantly more than expected, whereas they killed other livestock types in proportion to their abundance. The three villages combined incurred an estimated annual monetary loss of approximately $USD 12,120 amounting to approximately $USD 190/household/y. This relatively high total annual loss occurred primarily because of depredation of the most valuable livestock types such as yak and horse. Conservation actions should initially attempt to target decrease of predation on these large and valuable livestock species.

Surveillance of Echinococcus isolates from Qinghai, China.

PubMed

Ma, Junying; Wang, Hu; Lin, Gonghua; Zhao, Fang; Li, Chao; Zhang, Tongzuo; Ma, Xiao; Zhang, Yongguo; Hou, Zhibin; Cai, Huixia; Liu, Peiyun; Wang, Yongshun

2015-01-15

Echinococcosis is highly endemic over large parts of the Qinghai-Tibet Plateau (QTP), China. Based on a large number of samples, we present data on the current presence, host distribution, and genetic diversity of Echinococcus in the Qinghai Province, located in the northeastern corner of the QTP and constituting >25% of the area of the plateau. We used 521 samples (including 451 newly collected samples and 70 samples from our previous study) from humans, yaks, sheep, goats, dogs, fox, plateau pikas, and voles in 36 counties, and genotyped them using the mitochondrial DNA marker cytochrome oxidase subunit I (cox1) gene and the maximum parsimony and Bayesian reconstruction methods. Based on the 792 bp sequence matrix, we recorded 177 variable sites; 157 were parsimony-informative. A total of 105 haplotypes (H1-H105) were detected, of which H1-H15 and H90-H104, H16-H17, H18-H89, and H105 belonged to Echinococcus shiquicus, Echinococcus multilocularis, Echinococcus granulosus, and Echinococcus canadensis, respectively. Our results showed that, (i) the Qinghai Province was under a high burden of Echinococcus epidemiology; (ii) E. granulosus was the main echinococcosis threat to the local people, and the followed is E. multilocularis; (iii) there are a considerable number of haplotypes shared by domestic animals (sheep, yaks, and dogs) and humans, demonstrating the close relationship between human and domestic animals epidemiology; (iv) the threat of E. shiquicus on humans and livestock can be mostly ignored, while the infection risk of E. canadensis echinococcosis should not be neglected. Copyright © 2014 Elsevier B.V. All rights reserved.

Mapping wetland and forest landscapes in Siberia with Landsat data

NASA Astrophysics Data System (ADS)

Maksyutov, Shamil; Kleptsova, Irina; Glagolev, Mikhail; Sedykh, Vladimir; Kuzmenko, Ekaterina; Silaev, Anton; Frolov, Alexander; Nikolaeva, Svetlana; Fedorov, Alexander

2014-05-01

Landsat data availability provides opportunity for improving the knowledge of the Siberian ecosystems necessary for quantifying the response of the regional carbon cycle to the climate change. We developed a new wetland map based on Landsat data for whole West Siberia aiming at scaling up the methane emission observations. Mid-summer Landsat scenes were used in supervised classification method, based on ground truth data obtained during multiple field surveys. The method allows distinguishing following wetland types: pine-dwarf shrubs-sphagnum bogs or ryams, ridge-hollows complexes, shallow-water complexes, sedge-sphagnum poor fens, herbaceous-sphagnum poor fens, sedge-(moss) poor fens and fens, wooded swamps or sogra, palsa complexes. In our estimates wetlands cover 36% of the taiga area. Total methane emission from WS taiga mires is estimated as 3.6 TgC/yr,which is 77% larger as compared to the earlier estimate based on partial Landsat mapping combined with low resolution map due to higher fraction of fen area. We make an attempt to develop a forest typology system useful for a dynamic vegetation modeling and apply it to the analysis of the forest type distribution for several test areas in West and East Siberia, aiming at capability of mapping whole Siberian forests based on Landsat data. Test region locations are: two in West Siberian middle taiga (Laryegan and Nyagan), and one in East Siberia near Yakutsk. The ground truth data are based on analysis of the field survey, forest inventory data from the point of view of the successional forest type classification. Supervised classification was applied to the areas where ample ground truth and inventory data are available, using several limited area maps and vegetation survey. In Laryegan basin the upland forest areas are dominated (as climax forest species) by Scots pine on sandy soils and Siberian pine with presence of fir and spruce on the others. Those types are separable using Landsat spectral data alone. In the permafrost area around Yakutsk the most widespread succession type is birch to larch succession. Three stages of the birch to larch succession are detectable from Landsat image. When Landsat data is used in both West and East Siberia, distinction between deciduous broad-leaved species (birch, aspen, and willow) is difficult due to similarity in spectral signatures. Same problem exists for distinguishing between dark coniferous species (Siberian pine, fir and spruce). Forest classification can be improved by applying landscape type analysis, such as separation into floodplain, terrace, sloping hills.

Novel Serum Proteomic Signatures in a Non-Human Primate Model of Retinal Injury

DTIC Science & Technology

2011-01-01

2 SV=1 DB=tr 5 8 2 4 1.703409 0.496987 Q3YAK0 Phosphatidic acid phosphatase type 2 domain containing 1 (Fragment) OS=Macaca mulatta GN=PPAPDC1 PE=2...wash steps, as follows. In brief, gel pieces were incubated with 40% methanol + 7% acetic acid for 30 min, washed four times in water for 30 min under...from the gel pieces using 70% acetonitrile and 0.1% formic acid and then dried. Extracts were resuspended in 20 µl of 6 M guanidine-HCl, 5 mM potassium

Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from the rumen of yak, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov.

PubMed

Cai, Shichun; Dong, Xiuzhu

2010-04-01

An obligate anaerobic, Gram-staining-negative, mesophilic, cellulolytic bacterium, strain H1(T), was isolated from the rumen content of yak. Cells were straight to slightly curved rods, 0.8-1.0 x 3.0-4.0 microm in size, non-motile and encapsulated with mucous materials. Elliptical and terminal spores that swelled the cells were produced occasionally. The strain grew at 25-45 degrees C (optimum, 38 degrees C) and pH 6.0-7.8 (optimum, pH 6.7). Cellulose, cellobiose, xylan, xylose and maltose were used as carbon and energy sources, but not glucose. Products from cellulose and cellobiose fermentation were formic acid, acetic acid, carbon dioxide and trace amounts of ethanol, lactic acid and succinic acid. The genomic DNA G+C content was 33.7+/-1.2 mol%. The predominant fatty acids were C(16 : 0) (27.1 %), C(14 : 0) (9.2 %) and iso-C( 16 : 0) (6.4%). Based on the 16S rRNA gene sequence analysis, strain H1(T) was affiliated to the clostridial rRNA cluster XIVb and showed the highest 16S rRNA gene sequence similarity to Clostridium lentocellum DSM 5427(T) (96.0 %). These two strains formed a distinct lineage of the family 'Lachnospiraceae '. Based on data from this polyphasic taxonomic study, a new genus, Cellulosilyticum gen. nov., is proposed. Cellulosilyticum ruminicola sp. nov. is proposed for strain H1(T). The type strain of Cellulosilyticum ruminicola sp. nov. is strain H1(T) (=CGMCC 1.5065(T)=JCM 14822(T)). Clostridium lentocellum was reclassified in the new genus as Cellulosilyticum lentocellum comb. nov. (type strain RHM5(T)=ATCC 49066( T)=DSM 5427(T)=NCIMB 11756(T)).

Proteiniclasticum ruminis gen. nov., sp. nov., a strictly anaerobic proteolytic bacterium isolated from yak rumen.

PubMed

Zhang, Kegui; Song, Lei; Dong, Xiuzhu

2010-09-01

Two strictly anaerobic, proteolytic bacterial strains, designated strain D3RC-2(T) and D3RC-3r, were isolated from a cellulose-degrading mixed culture enriched from yak rumen content. The strains were Gram-stain negative and non-spore-forming with cell sizes of 0.5-0.8 x 0.6-2.0 mum. The temperature range for growth was 24-46 degrees C (optimum 38-39 degrees C) and the pH range was between 5.6 and 8.7 (optimum 7.0-7.3). Both strains used soya peptone, tryptone, l-phenylalanine, l-leucine, l-methionine, l-serine, l-valine, l-threonine and l-histidine as carbon and nitrogen sources, but did not use any of the saccharides tested. The major fermentation products from PY medium were acetate, propionate and iso-butyrate. The DNA G+C contents of strains D3RC-2(T) and D3RC-3r were 41.0+/-0.1 mol% and 41.3+/-0.1 mol% (HPLC), respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains represented a new phyletic sublineage within the family Clostridiaceae, with <93.8 % 16S rRNA gene sequence similarity to recognized species. On the basis of the phenotypic, genotypic and physiological evidence, strains D3RC-2(T) and D3RC-3r are proposed as representing a novel species of a new genus, for which the name Proteiniclasticum ruminis gen. nov., sp. nov. is proposed. The type strain of the type species is D3RC-2(T) (=AS 1.5057(T)=JCM 14817(T)).

First description of the endogenous life cycle of Hypoderma sinense affecting yaks and cattle in China.

PubMed

Otranto, D; Paradies, P; Testini, G; Lia, R P; Giangaspero, A; Traversa, D; Colwell, D D

2006-09-01

Larvae belonging to five species of Hypoderma spp. (Diptera, Oestridae) cause myiasis in wild and domestic ruminants that is characterized by migrations within deep tissues. In China hypodermosis is one of the most important arthropod diseases affecting ruminants and, moreover, represents a significant zoonosis, with numerous reports of Hypoderma spp. affecting farmers. Recently, a sixth species, Hypoderma sinense Pleske, has been rediscovered but the endogenous migration pathway within the host body is completely unknown and it represents a major constraint for the control of larval infection. In December 2003 a total of 165 larval stages of Hypoderma spp. were collected from different anatomical sites of 40 yaks slaughtered at an abattoir in the province of Gansu, China. The morphological characters and size of the recovered larvae were used to infer migratory routes and 45 specimens were also subjected to a polymerase chain reaction (PCR) assay of cox1 mtDNA and amplicons sequenced. All the larvae molecularly processed were identified as H. sinense and sequence identity was confirmed by a PCR-restriction fragment length polymorphism (PCR-RFLP) tool carried out using BfaI and HinfI endonucleases. The finding of H. sinense larvae only in the oesophagus or both in oesophagus and subcutaneous tissue of 12 and 15 animals, respectively, indicates that H. sinense larvae migrate through the oesophagus similarly to Hypoderma lineatum (De Villers). The description of the endogenous life cycle of H. sinense will help to determine the timing of specific treatment programmes to guarantee the improvement of animal welfare and health, thus resulting in an increase in livestock production in China.

Bacterial microbiota compositions of naturally fermented milk are shaped by both geographic origin and sample type.

PubMed

Zhong, Z; Hou, Q; Kwok, L; Yu, Z; Zheng, Y; Sun, Z; Menghe, B; Zhang, H

2016-10-01

Naturally fermented dairy products contain a rich microbial biodiversity. This study aimed to provide an overview on the bacterial microbiota biodiversity of 85 samples, previously collected across a wide region of China, Mongolia, and Russia. Data from these 85 samples, including 55 yogurts, 18 naturally fermented yak milks, 6 koumisses, and 6 cheeses, were retrieved and collectively analyzed. The most prevalent phyla shared across samples were Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria, which together accounted for 99% of bacterial sequences. The predominant genera were Lactobacillus, Lactococcus, Streptococcus, Acetobacter, Acinetobacter, Leuconostoc, and Macrococcus, which together corresponded to 96.63% of bacterial sequences. Further multivariate statistical analyses revealed significant differences in the microbiota structure across sample geographic origin and type. First, on the principal coordinate score plot, samples representing the 3 main sample collection regions (Russia, Xinjiang, and Tibet) were mostly located respectively in the upper left, lower right, and lower left quadrants, although slight overlapping occurred. In contrast, samples from the minor sampling areas (Inner Mongolia, Mongolia, Gansu, and Sichuan) were predominantly distributed in the lower left quadrant. These results suggest a possible association between sample geographical origin and microbiota composition. Second, bacterial microbiota structure was stratified by sample type. In particular, the microbiota of cheese was largely distinct from the other sample types due to its high abundances of Lactococcus and Streptococcus. The fermented yak milk microbiota was most like that of the yogurts. Koumiss samples had the lowest microbial diversity and richness. In conclusion, both geographic origin and sample type shape the microbial diversity of naturally fermented milk. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

[Study of biological value of beef produced by interspecies hybrids of domestic cattle and wild yaks].

PubMed

Bagirov, V A; Chernukha, I M; Lisitsin, A V; Zinovieva, N A

2014-01-01

The comparative study of the chemical composition and biological values of beef produced by hybrids of Angus cattle with wild yaks (hybrid beef) and pure-bred Angus cattle (traditional beef) has been carried out. Longissimus muscle samples were used for analysis. It was observed, that the hybrid beef samples had the practically equal protein content comparing to traditional beef (21.1 vs. 21.6 per cent) but were characterized by the lower fat content (1.2 vs. 2.5 per cent). The higher biological value of hybrid beef comparing to traditional beef has been shown. The value of protein-quality index, calculated as the ratio of tryptophan amino acid to oxyprolin and characterizing the ratio of high biological value proteins to low biological value proteins was 8.1 vs. 5.7. The values of amino acid indexes [ratio of essential amino acids (EAA) to non-essential amino acids (NAA) and ratio of EAA to the total amount of amino acids (TAA)] were EAA/NAA = 0.77 vs. 0.65 and EAA/TAA = 0.43 vs. 0.39. The protein of hybrid beef was characterized by the higher content of a number of the essential amino acids: by a factor of 1, 77 for threonin, 1.23--for valin, 1.09--for lysin, 1.17--for leucine and 1.19--for tryptophan. The amount of the essential amino acids in 1 gram of protein of the hybrid beef was 434.7 mg against 393.1 mg for traditional beef It has been shown, that the protein of the hybrid beef comparing to traditional beef is characterized by the higher values of the amino acid scores calculated for EAA.

Offshore Tectonics of the St. Elias Mountains: Insights from Ocean Drilling and Seismic Stratigraphy on the Yakutat Shelf

NASA Astrophysics Data System (ADS)

Worthington, L. L.; Gulick, S. P. S.; Montelli, A.; Jaeger, J. M.; Zellers, S.; Walczak, M. H.; Mix, A. C.

2015-12-01

Ongoing collision of the Yakutat (YAK) microplate with North America (NA) in southern Alaska has driven orogenesis of the St. Elias Mountains and the advance of the offshore deformation front to the southeast. The offshore St. Elias fold-thrust belt records the complex interaction between collisional tectonics and glacial climate variability, providing insight for models of orogenesis and the evolution of glacial depocenters. Glacial erosion and deposition have provided sediment that constructed the upper continental shelf, much of which has been reincorporated into the orogenic wedge through offshore faulting and folding. We integrate core and downhole logging data from IODP Expedition 341 (Sites U1420 and U1421) drilled on the Yakutat shelf and slope with high-resolution and regional seismic profiles to investigate the coupled structural and stratigraphic evolution of the St. Elias margin. Site U1420 lies on the Yakutat shelf within the Bering Trough, a shelf-crossing trough that is within primary depocenter for Bering Glacier sediments. Two faults underlie the glacial packages and have been rendered inactive as the depositional environment has evolved, while faulting elsewhere on the shelf has initiated. Site U1421 lies on the current continental slope, within the backlimb of an active thrust that forms part of the modern YAK-NA deformation front. At each of these sites, we recovered glacigenic diamict (at depths up to ~1015 m at Site U1420), much of which is younger than 0.3 Ma. Age models within the trough indicated that initiation of active deformation away from the Bering Trough depocenter likely occurred since 0.3 Ma, suggesting that possible tectonic reorganization due to mass redistribution by glacial processes can occur at time scales on the order of 100kyr-1Myr.

Sphingobacterium bovisgrunnientis sp. nov., isolated from yak milk.

PubMed

Kaur, Manpreet; Singh, Harjodh; Sharma, Shivani; Mishra, Sunita; Tanuku, Naga Radha Srinivas; Pinnaka, Anil Kumar

2018-02-01

A novel Gram-negative, rod shaped, non-motile bacterium, designated strain YK2 T , was isolated from yak milk from Leh, India. The strain was positive for oxidase- and catalase-activities and negative for starch hydrolysis, nitrate reduction, citrate utilization, urease, lysine decarboxylase and ornithine decarboxylase activities. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C17 : 1ω9c and C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH (summed feature 3). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids. The DNA G+C content of the strain was 38.9 mol%. The 16S rRNA gene sequence analysis indicated that strain YK2 T was a member of the genus Sphingobacterium and closely related to Sphingobacterium alimentarium and Sphingobacterium composti with pair-wise sequence similarity of 98.3 and 97.9 %, respectively. The sequence similarity to other members of the genus Sphingobacterium was between 92.6 to 96.3 %. Phylogenetic analysis showed that strain YK2 T clustered with Sphingobacterium alimentarium and together clustered with Sphingobacterium composti. DNA-DNA hybridization of strain YK2 T with Sphingobacterium alimentarium WCC 4521 T and Sphingobacterium composti T5-12 T showed a relatedness of only 38 and 54 %, respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain YK2 T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium bovisgrunnientis sp. nov. is proposed. The type strain of Sphingobacterium bovisgrunnientis sp. nov. is YK2 T (=MTCC 12631 T =KCTC 52685 T =JCM 31951 T ).

Distribution of trace gases and aerosols in the troposphere over West Siberia and Kara Sea

NASA Astrophysics Data System (ADS)

Belan, Boris D.; Arshinov, Mikhail Yu.; Paris, Jean-Daniel; Nédélec, Philippe; Ancellet, Gérard; Pelon, Jacques; Berchet, Antoine; Arzoumanian, Emmanuel; Belan, Sergey B.; Penner, Johannes E.; Balin, Yurii S.; Kokhanenko, Grigorii; Davydov, Denis K.; Ivlev, Georgii A.; Kozlov, Artem V.; Kozlov, Alexander S.; Chernov, Dmitrii G.; Fofonov, Alexader V.; Simonenkov, Denis V.; Tolmachev, Gennadii

2015-04-01

The Arctic is affected by climate change much stronger than other regions of the globe. Permafrost thawing can lead to additional methane release, which enhances the greenhouse effect and warming, as well as changes of Arctic tundra ecosystems. A great part of Siberian Arctic is still unexplored. Ground-based investigations are difficult to be carried out in this area due to it is an out-of-the-way place. So, in spite of the high cost, aircraft-based in-situ measurements can provide a good opportunity to fill up the gap in data on the atmospheric composition over this region. The ninth YAK-AEROSIB campaign was focused on the airborne survey of Arctic regions of West Siberia. It was performed in October 2014. During the campaign, the high-precision in-situ measurements of CO2, CH4, CO, O3, black carbon and aerososls, including aerosol lidar profiles, have been carried out in the Siberian troposphere from Novosibirsk to Kara Sea. Vertical distributions of the above atmospheric constituents will be presented. This work was supported by LIA YAK-AEROSIB, CNRS (France), the French Ministry of Foreign Affairs, CEA (France), the Branch of Geology, Geophysics and Mining Sciences of RAS (Program No. 5); State contracts of the Ministry of Education and Science of Russia No. 14.604.21.0100, (RFMTFIBBB210290) and No. 14.613.21.0013 (RFMEFI61314X0013); Interdisciplinary integration projects of the Siberian Branch of the Russian Academy of Science No. 35, No. 70 and No. 131; and Russian Foundation for Basic Research (grants No. 14-05-00526 and 14-05-00590).

Human beings' adaptability to extreme environmental changes from medical and physical points of view

NASA Astrophysics Data System (ADS)

Khabarova, Olga; Ragulskaya, Maria; Dimitrova, Svetla; Safaraly-Oghlu Babayev, Elchin; Samsonov, Sergey; Med. Dimitry Markov, Of; Nazarova, Of Med. Olga N.; Rudenchik, Evgeny

The question about features of human reaction on the sharp environmental physical activity (EPA) changes is considered by international group of physicists and physicians on the base of results of monitoring of human health state in different cities spread on latitude and longitude. The typical reaction of human body on the influences, exceeding the organisms' ability to adaptation, is of stress-reaction character. From medical point of view there is no significant difference for human body -what external (EPA) agent shocked an organism (emotional or some physical threats). First attempt of the organism to restore its homeostasis is stress-reaction, being universal for many stress-factors. Its main stages (such as alarm, resistance, and exhaustion) are detectable by different medical equipments, but we tried to find universal, non-traumatic method of daily measurements, enough sensitive and appropriate for observation of people reaction both on weather and space weather (geomagnetic activity) changes. The experiment was based on a method of electrical conductivity measurements of biologically active (acupunctural) points of human skin. The used method (electroacupunctural method by Dr. R.Voll) is very sensitive to current state of an organism and characterize the functional condition of different organs and systems of human body and allows to express so-called "group's health status" in the units, suitable for comparison with meteorological and heliogeophysical parameters. We conduct the parallel investigations as a part of collaborative study in different geographic latitudes-longitudes (Baku:40° 23'43"N -49° 52'56"E, Troitsk (Moscow region): 55° 28'40"N -37° 18'42"E, Yakutsk: 62° 02'00"N -129° 44'00"E). Measurements were carried out on daily basis with permanent group of functionally healthy persons (Moscow -19, Yakutsk -22, CityBaku -12 volunteers). Daily monitoring of nervous, endocrinological, lymphatic systems, blood, lungs, thick and thin intestine, heart and parenhimatic organs, allergy and hypophisis was conducted simultaneously with analyses of space weather (parameters of solar and geomagnetic activities) as well as local meteorological parameters (temperature, atmospheric pressure, humidity, wind speed, etc.). It was proved that it is possible to consider not only weather changes but also geomagnetic field variations as a stressor. It is concluded that : 1. human reaction on the sharp changes of selected external (environmental) physical activity parameters goes like typical stress-reaction; 2. features of stress-reaction depend on history of previous failures of an organism and on state of external background (frequent stresses deplete human organism possibility to adaptation); 3. features of stress-reaction depend on the geographic location (latitude). Possible physi-cal explanation of human organism stress-reaction on changes of geomagnetic oscillatory regime and atmospheric thermobaric variations is discussed.

Diversity of lactic acid bacteria associated with traditional fermented dairy products in Mongolia.

PubMed

Yu, J; Wang, W H; Menghe, B L G; Jiri, M T; Wang, H M; Liu, W J; Bao, Q H; Lu, Q; Zhang, J C; Wang, F; Xu, H Y; Sun, T S; Zhang, H P

2011-07-01

Spontaneous milk fermentation has a long history in Mongolia, and beneficial microorganisms have been handed down from one generation to the next for use in fermented dairy products. The objective of this study was to investigate the diversity of lactic acid bacteria (LAB) communities in fermented yak, mare, goat, and cow milk products by analyzing 189 samples collected from 13 different regions in Mongolia. The LAB counts in these samples varied from 3.41 to 9.03 log cfu/mL. Fermented yak and mare milks had almost identical mean numbers of LAB, which were significantly higher than those in fermented goat milk but slightly lower than those in fermented cow milk. In total, 668 isolates were obtained from these samples using de Man, Rogosa, and Sharpe agar and M17 agar. Each isolate was considered to be presumptive LAB based on gram-positive and catalase-negative properties, and was identified at the species level by 16S rRNA gene sequencing, multiplex PCR assay, and restriction fragment length polymorphism analysis. All isolates from Mongolian dairy products were accurately identified as Enterococcus faecalis (1 strain), Enterococcus durans (3 strains), Lactobacillus brevis (3 strains), Lactobacillus buchneri (2 strains), Lactobacillus casei (16 strains), Lactobacillus delbrueckii ssp. bulgaricus (142 strains), Lactobacillus diolivorans (17 strains), Lactobacillus fermentum (42 strains), Lactobacillus helveticus (183 strains), Lactobacillus kefiri (6 strains), Lactobacillus plantarum ssp. plantarum (7 strains), Lactococcus lactis ssp. lactis (7 strains), Leuconostoc lactis (22 strains), Leuconostoc mesenteroides (21 strains), Streptococcus thermophilus (195 strains), and Weissella cibaria (1 strain). The predominant LAB were Strep. thermophilus and Lb. helveticus, which were isolated from all sampling sites. The results demonstrate that traditional fermented dairy products from different regions of Mongolia have complex compositions of LAB species. Such diversity of LAB provides useful information for further studies of probiotic strain selection and starter culture design, with regard to the industrial production of traditional fermented milk. Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Physicochemical characterization and antitumour activity of exopolysaccharides produced by Lactobacillus casei SB27 from yak milk.

PubMed

Di, Wei; Zhang, Lanwei; Wang, Shumei; Yi, Huaxi; Han, Xue; Fan, Rongbo; Zhang, Yingchun

2017-09-01

Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China. GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated. The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively. Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively. Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain. Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure. The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes. Finally, TEM images revealed the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2. Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs. Copyright © 2017 Elsevier Ltd. All rights reserved.

Early Human Occupation on the Northeast Tibetan Plateau

NASA Astrophysics Data System (ADS)

Rhode, D.; Madsen, D.; Brantingham, P.; Perrault, C.

2010-12-01

The Tibetan Plateau presents great challenges for human occupation: low oxygen, high ultraviolet radiation, harsh seasonal climate, low overall biological productivity. How and when early humans were able to cope physiologically, genetically, and behaviorally with these extremes is important for understanding the history of human adaptive flexibility. Our investigations of prehistoric human settlement on the northeast Tibetan Plateau focus on (a) establishing well-dated evidence for occupation of altitudes >3000 m, (b) the environmental context of high altitude adaptation, and (c) relations of hunting and pastoralism to lower-altitude agrarian systems. We observe two major prehistoric settlement patterns in the Qinghai Lake area. The earliest, ~15,000-7500 yr old, consists of small isolated firehearths with sparse associated stone tools and wild mammal remains (1). Numerous hearths often occur in the same localities, indicating repeated short-duration occupations by small hunting parties. A second pattern, ~9000-4000 yr old, was established during the Holocene climatic optimum. These sites represent prolonged seasonal residential occupation, containing dark anthropogenic midden, hearth and pit constructions, abundant stone tools, occasional ceramics, and abundant diverse faunal remains (including medium-large mammals but lacking domestic sheep/yak)(2). These Plateau-margin base camps allowed greater intensity of use of the high Plateau. Residential occupation was strongly influenced by nearby lower-altitude farming communities; development of the socioeconomic landscape along the Yellow River likely played at least as great a role in Plateau occupation patterns as did Holocene environmental changes. Holocene vegetation changes in the NE Tibetan Plateau have been attributed to climate (3) or anthropogenic modification (4). Our results document changes in shrub/tree presence from ~12,000-4000 BP, similar to pollen records, that likely reflect climate rather than anthropogenic grazing pressure, because domestic yak/sheep remains are absent archaeologically before ~4000 BP. Our results have implications for the age of distinct Tibetan high-altitude physiological adaptations (5), suggested to be as recent as ~2750 BP. Prolonged occupation of Plateau margins commenced much earlier, but year-round occupation above 4000 m likely occurred only with the emergence of yak pastoralism (6). Genetic models need to consider which environmental factors lead to strong selection for genetic divergence (e.g., seasonal vs year-round occupation, occupation at ~3000 m vs >4000 m, etc); settlement history reconstructions such as that presented here can help. (1)Brantingham PJ, et al. 2007, Elsevier Dev Quat Sci 9:129-150; Madsen DB, et al. 2006, J Arch Sci 33:1433-1444. (2)Rhode D, et al. 2007, J Arch Sci 34:600-612. (3)Herzschuh, U, et al. 2010, Glo Ecol Biogeog 19:278-286. (4)Miehe G, et al. 2009, Palaeo Palaeo Palaeo 267:130-147.; Schlütz F., Lehmkuhl F., 2009, Quat Sci Rev 28:1449-1471. (5)Beall C, et al. 2010, PNAS 107:11459-11464; Simonson TS, et al. 2010, Science 329:72-75; Yi X. et al., 2010, Science 329:75-78. (6)Rhode D, et al. 2007, Elsevier Dev Quat Sci 9:205-226.

Modeling of larch forest dynamics under a changing climate in eastern Siberia

NASA Astrophysics Data System (ADS)

Nakai, T.; Kumagai, T.; Iijima, Y.; Ohta, T.; Kotani, A.; Maximov, T. C.; Hiyama, T.

2017-12-01

According to the projection by an earth system model under RCP8.5 scenario, boreal forest in eastern Siberia (near Yakutsk) is predicted to experience significant changes in climate, in which the mean annual air temperature is projected to be positive and the annual precipitation will be doubled by the end of 21st century. Since the forest in this region is underlain by continuous permafrost, both increasing temperature and precipitation can affect the dynamics of forest through the soil water processes. To investigate such effects, we adopted a newly developed terrestrial ecosystem dynamics model named S-TEDy (SEIB-DGVM-originated Terrestrial Ecosystem Dynamics model), which mechanistically simulates "the way of life" of each individual tree and resulting tree mortality under the future climate conditions. This model was first developed for the simulation of the dynamics of a tropical rainforest in the Borneo Island, and successfully reproduced higher mortality of large trees due to a prolonged drought induced by ENSO event of 1997-1998. To apply this model to a larch forest in eastern Siberia, we are developing a soil submodel to consider the effect of thawing-freezing processes. We will present a simulation results using the future climate projection.

Cosmic rays from 1017eV to beyond 1020eV: evidence from the fly's eye experiment and ground arrays.

NASA Astrophysics Data System (ADS)

Sokolsky, P.

The study of cosmic rays with energies well above the knee (1015eV) of the spectrum has a long history. Beginning with the pioneering work of Linsley and the Volcano Ranch array, followed by the SUGAR, Haverah Park, Yakutsk, Akeno and AGASA array and the Fly's Eye detector, evidence has been steadily accumulating that the spectrum exhibits a change in slope near 10×1018eV. This flattening may indicate that the spectrum in this region is largely extragalactic in origin. If the sources of this spectrum are sufficiently distant, a cut-off due to the interaction of near 1020eV protons with black body photons should be observed. Recent data from the AGASA and Fly's Eye detectors have brought to bear information of spectral shape, composition and anisotropy to this study. In addition to clarifying the nature of the spectral structure, these new results lead to the surprising conclusion that nearby cosmic ray sources must exist that produce particles with energies well in excess of 1020eV. New experiments are being designed which will have the enormous apertures necessary for detecting these very rare events.

New estimates of extensive-air-shower energies on the basis of signals in scintillation detectors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anyutin, N. V.; Dedenko, L. G., E-mail: ddn@dec1.sinp.msu.ru; Roganova, T. M.

New formulas for estimating the energy of inclined extensive air showers (EASs) on the basis of signals in detectors by means of an original method and detailed tables of signals induced in scintillation detectors by photons, electrons, positrons, and muons and calculated with the aid of the GEANT4 code package were proposed in terms of the QGSJETII-04, EPOS LHC, and GHEISHA models. The parameters appearing in the proposed formulas were calculated by employing the CORSIKA code package. It is shown that, for showers of zenith angles in the range of 20◦–45◦, the standard constant-intensity-cut method, which is used to interpretmore » data from the Yakutsk EAS array, overestimates the shower energy by a factor of 1.2 to 1.5. It is proposed to employ the calculated VEM (Vertical Equivalent Muon) signal units of 10.8 and 11.4 MeV for, respectively, ground-based and underground scintillation detectors and to take into account the dependence of signals on the azimuthal angle of the detector position and fluctuations in the development of showers.« less

Applying wavelet transforms to analyse aircraft-measured turbulence and turbulent fluxes in the atmospheric boundary layer over eastern Siberia

NASA Astrophysics Data System (ADS)

Strunin, M. A.; Hiyama, T.

2004-11-01

The wavelet spectral method was applied to aircraft-based measurements of atmospheric turbulence obtained during joint Russian-Japanese research on the atmospheric boundary layer near Yakutsk (eastern Siberia) in April-June 2000. Practical ways to apply Fourier and wavelet methods for aircraft-based turbulence data are described. Comparisons between Fourier and wavelet transform results are shown and they demonstrate, in conjunction with theoretical and experimental restrictions, that the Fourier transform method is not useful for studying non-homogeneous turbulence. The wavelet method is free from many disadvantages of Fourier analysis and can yield more informative results. Comparison of Fourier and Morlet wavelet spectra showed good agreement at high frequencies (small scales). The quality of the wavelet transform and corresponding software was estimated by comparing the original data with restored data constructed with an inverse wavelet transform. A Haar wavelet basis was inappropriate for the turbulence data; the mother wavelet function recommended in this study is the Morlet wavelet. Good agreement was also shown between variances and covariances estimated with different mathematical techniques, i.e. through non-orthogonal wavelet spectra and through eddy correlation methods.

Bos grunniens and Bos mutus (Artiodactyla: Bovidae)

USGS Publications Warehouse

Leslie, David M.; Schaller, George B.

2009-01-01

Bos grunniens Linnaeus, 1766, and Bos mutus (Przewalski, 1883) are the domestic and wild forms, respectively, of the bovid commonly called the yak. B. mutus inhabits remote high-elevation alpine meadows and alpine steppe in rolling to mountainous terrain in the Tibetan Plateau, and B. grunniens is maintained widely in China and other parts of Central Asia, and uncommonly elsewhere in the world. Populations of B. mutus are substantially reduced and fragmented throughout its remaining range; the largest numbers occur in northern Tibet and western Qinghai. B. mutus is vulnerable because of poaching and competition with domestic livestock. Although no complete survey of B. mutus has been conducted, there are probably no more than 15,000 remaining in remote areas of the Tibetan Plateau; B. grunniens numbers about 14 million.

Molecular docking revealed the binding of nucleotide/side inhibitors to Zika viral polymerase solved structures.

PubMed

Elfiky, A A; Ismail, A M

2018-05-01

A new Zika virus (ZIKV) outbreak started in 2015. According to the World Health Organization, 84 countries confirmed ZIKV infection. RNA-dependent RNA polymerase (RdRp) was an appealing target for drug designers during the last two decades. Through molecular docking, we screened 16 nucleotide/side inhibitors against ZIKV RdRp. While the mode of interaction with ZIKV is different from that in the hepatitis C virus (HCV), nucleotide/side inhibitors in this study (mostly anti-HCV) showed promising binding affinities (-6.2 to -9.7 kcal/mol calculated by AutoDock Vina) to ZIKV RdRp. Setrobuvir, YAK and, to a lesser extent, IDX-184 reveal promising results compared to other inhibitors in terms of binding ZIKV RdRp. These candidates would be powerful anti-ZIKV drugs.

Distribution of Trace Gases and Aerosols in the Troposphere Over Siberia During Wildfires of Summer 2012

NASA Astrophysics Data System (ADS)

Antokhin, P. N.; Arshinova, V. G.; Arshinov, M. Y.; Belan, B. D.; Belan, S. B.; Davydov, D. K.; Ivlev, G. A.; Fofonov, A. V.; Kozlov, A. V.; Paris, J.-D.; Nedelec, P.; Rasskazchikova, T. M.; Savkin, D. E.; Simonenkov, D. V.; Sklyadneva, T. K.; Tolmachev, G. N.

2018-02-01

The results of sensing of the gas and aerosol composition of the atmosphere with the Optik Tu-134 aircraft laboratory in the period from 31 July to 1 August 2012 are presented. The measurements were conducted along the flight route Novosibirsk-Tomsk-Mirny-Yakutsk-Bratsk-Novosibirsk. A significant part of the Siberian territory during this period was covered by numerous forest fires. The synoptic situation during the measurements was characterized by the presence of low-gradient field. This fact determined the low rate of transport and diffusion of pollutants and their accumulation in the region under study. The maximal concentrations of CO2, CH4, and CO over fire zones achieved 432 ppm, 2367 ppb, and 4036 ppb, respectively. The aerosol particle number density in emission plumes achieved 4400 cm-3. Outside emission plumes, the concentration ranged within 400-1000 cm-3 depending on the region. The mass concentration of aerosol in plumes increased approximately 7 times (6.9). The enrichment of the concentration of some elements and ions in the plume with respect to the background varied from 1.3 to 9.1 times. The generation of ozone from biomass burning products was observed at plume boundaries. Two versions of this process are possible: ozone generation under and above the plume.

Spatial relationship with the grazing pressure and alpine grassland degradation base on the GPS tracing experiment: a case study in the source region of Yellow River

NASA Astrophysics Data System (ADS)

Liu, L.; Zhang, Y.

2014-12-01

It is hard to distinguish the affects from the human activities and climate change on the grassland degradation, especially to quantification the human activities. Grazing is the main kind of human activities on the alpine grassland. To investigate the relationship of the grazing activities and grassland degradation will help the understanding of the effects of human impacts. A GPS tracing experiment was designed to tracing the activities of the Yaks. The spatial distribution of the grazing pressure was simulated under Geographic Information System. The biomass distribution and grazing pressure was compared to judge the distribution of overgrazing. The main research results are: (1) The tracing experiment could record the track of the Yaks very well, and it could be a good tool for the quantification research of grazing pressure. (2) The grazing activities have good relationship with vegetation, residents and landform. The worse vegetation is, the grazing time is longer and the radius is bigger. The closer to the residents, the grazing intensive is higher. The grazing route is influenced by the landform. Usually the herds would like to choose the least cost way. And the grazing intensive is higher in sunny slope. (3) The grazing probability is higher while the elevation is lower and the vegetation is better. The numbers of livestock of different villages determine the spatial distribution of the grazing pressure but it has very big heterogeneity in the same village. It seems the forage is enough in the research area since the available biomass is about 1190058t and the total demand is only 603700t. But because of the heterogeneity of the grazing pressure, there are still overgrazing in some villages. The area of overgrazing is 6 percent in the winter rangeland and 11 percent in summer rangeland. It is important to take care of the spatial heterogeneity of the grazing pressure and grass production in the management of the grassland ecosystem. It should improve the calculation method of the stock capacity to avoid the overrating of it. To arrange the livestock reasonably is the guarantee of the sustainable development in this region.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Garige, Mamatha; Walters, Eric, E-mail: ewalters@howard.edu

The molecular basis for nutraceutical properties of the polyphenol curcumin (Curcuma longa, Turmeric) is complex, affecting multiple factors that regulate cell signaling and homeostasis. Here, we report the effect of curcumin on cellular and developmental mechanisms in the eukaryotic model, Dictyostelium discoideum. Dictyostelium proliferation was inhibited in the presence of curcumin, which also suppressed the prestarvation marker, discoidin I, members of the yakA-mediated developmental signaling pathway, and expression of the extracellular matrix/cell adhesion proteins (DdCAD and csA). This resulted in delayed chemotaxis, adhesion, and development of the organism. In contrast to the inhibitory effects on developmental genes, curcumin induced gstAmore » gene expression, overall GST activity, and generated production of reactive oxygen species. These studies expand our knowledge of developmental and biochemical signaling influenced by curcumin, and lends greater consideration of GST enzyme function in eukaryotic cell signaling, development, and differentiation.« less

Superfund Record of Decision (EPA Region 8): California Gulch, Operable Unit 10, Leadville, CO, August 8, 1997

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1998-01-01

This decision document presents the Selected Remedy for Oregon Gulch OU10 within the California Gulch Superfund Site in Leadville, Colorado. The Selected Remedy includes active management of the seep currently discharging at the toe of the Oregon Gulch Tailing Impoundment during the interim period from implementation until the seep does not negatively impact surface water quality. Active management of the seep discharge will be performed during non-freezing conditions and will include collection and either pumping or transport of the collected flow to the Yak Tunnel Treatment Plant or other suitable treatment options. Design of the Selected Remedy will include amore » drain system at the toe of the embankment to allow the seep discharge to flow unrestricted and to be collected in a controlled manner.« less

Animal Fascioliasis: Perspectives from high altitudinal regions.

PubMed

Lyngdoh, Damanbha; Sharma, Sunil; Roy, Bishnupada; Tandon, Veena

2016-12-15

The parasitic flukes of the genus Fasciola (Platyhelminthes: Trematoda: Digenea) cause fascioliasis or liver-rot disease in ruminant livestock in tropical and sub-tropical regions of the world. Classically, two species of Fasciola- F. hepatica and F. gigantica, are universally recognized as taxonomically valid species. Our survey studies on ovid and bovid animals including yak and mithun from high altitudinal mountainous regions in Northeast India revealed the occurrence of Fasciola gigantica and also Fasciola sp.- an intermediate form, at altitudes between 5000 and 14,085 feet above sea level (asl). Two morphotypes- F. hepatica - like and F. gigantica - like, of Fasciola species were reported from the high altitudinal areas of Northeast India; most of these locales constitute new-locality and first records for the occurrence of these liver flukes. Copyright © 2016 Elsevier B.V. All rights reserved.

A pseudoreceptor modelling study of the varicella-zoster virus and human thymidine kinase binding sites

NASA Astrophysics Data System (ADS)

Greenidge, Paulette A.; Merz, Alfred; Folkers, Gerd

1995-12-01

A representative range of pyrimidine nucleoside analogues that are known to inhibit herpes simplex virus (HSV) replication have been used to construct receptor binding site models for the varicella-zoster virus (VZV), thymidine kinase (TK) and human TK1. Given a set of interacting ligands, superimposed in such a manner as to define a pharmacophore, the pseudoreceptor modelling technique Yak provides a means of building binding site models of macromolecules for which no three-dimensional experimental structures are available. Once the models have been evaluated by their ability to reproduce experimental binding data [Vedani et al., J. Am. Chem. Soc., 117 (1995) 4987], they can be used for predictive purposes. Calculated and experimental values of relative binding affinity are compared. Our models suggest that the substitution of one residue may be sufficient to determine ligand subtype affinity.

[Perception of odor quality by Free Image-Association Test].

PubMed

Ueno, Y

1992-10-01

A method was devised for evaluating odor quality. Subjects were requested to freely describe the images elicited by smelling odors. This test was named the "Free Image-Association Test (FIT)". The test was applied for 20 flavors of various foods, five odors from the standards of T&T olfactometer (Japanese standard olfactory test), butter of yak milk, and incense from Lamaism temples. The words for expressing imagery were analyzed by multidimensional scaling and cluster analysis. Seven clusters of odors were obtained. The feature of these clusters were quite similar to that of primary odors which have been suggested by previous studies. However, the clustering of odors can not be explained on the basis of the primary-odor theory, but the information processing theory originally proposed by Miller (1956). These results support the usefulness of the Free Image-Association Test for investigating odor perception based on the images associated with odors.

Structure-activity relationships of cannabinoids: A joint CoMFA and pseudoreceptor modelling study

NASA Astrophysics Data System (ADS)

Schmetzer, Silke; Greenidge, Paulette; Kovar, Karl-Artur; Schulze-Alexandru, Meike; Folkers, Gerd

1997-05-01

A cannabinoid pseudoreceptor model for the CB1-receptor has been constructed for 31 cannabinoids using the molecular modelling software YAK. Additionally, two CoMFA studies were performed on these ligands, the first of which was conducted prior to the building of the pseudoreceptor. Its pharmacophore is identical with the initial superposition of ligands used for pseudoreceptor construction. In contrast, the ligand alignment for the second CoMFA study was taken directly from the final cannabinoid pseudoreceptor model. This altered alignment gives markedly improved cross-validated r2 values as compared to those obtained from the original alignment with{{r}}_{{{cross}}}^2 values of 0.79 and 0.63, respectively, for five components. However, the pharmacophore alignment has the better predictive ability. Both the CoMFA and pseudoreceptor methods predict the free energy of binding of test ligands well.

From yaks to yogurt: the history, development, and current use of probiotics.

PubMed

McFarland, Lynne V

2015-05-15

The development of probiotics, which are living bacteria or yeasts used to confer a health benefit on the host, has paralleled our research in food preservation, microbiologic identification techniques, and our understanding of how the complex interactions in microbiota impact the host's health and recovery from disease. This review briefly describes the history of probiotics, where probiotic strains were originally isolated, and the types of probiotic products currently available on the global market. In addition, the uses or indications for these probiotics are described, along with the types of clinical investigations that have been done. Continuing challenges persist for the proper probiotic strain identification, regulatory pathways, and how healthcare providers can choose a specific strain to recommend to their patients. © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Characterization of chiral amino acids from different milk origins using ultra-performance liquid chromatography coupled to ion-mobility mass spectrometry

NASA Astrophysics Data System (ADS)

Tian, He; Zheng, Nan; Li, Songli; Zhang, Yangdong; Zhao, Shengguo; Wen, Fang; Wang, Jiaqi

2017-04-01

Milk contains free amino acids (AAs) that play essential roles in maintaining the growth and health of infants, and D-AA isomers are increasingly being recognized as important signalling molecules. However, there are no studies of the different characteristics of chiral AA (C-AA) from different milk origins. Here, UPLC coupled to ion-mobility high-resolution MS (IM-HRMS) was employed to characterize 18 pairs of C-AAs in human, cow, yak, buffalo, goat, and camel milk. The results proved that milk origins can be differentiated based on the D- to L- AA ratio-based projection scores by principal component analysis. The present study gives a deeper understanding of the D- to L- AA ratio underlying the biological functions of different animal milks, and provide a new strategy for the study of AA metabolic pathways.

El proyecto AUGER

NASA Astrophysics Data System (ADS)

Etchegoyen, A.

Hace ya más de 30 años en Volcano Ranch, EE.UU., un extenso chubasco cósmico (ECC) fue detectado con energía en exceso de 1020 eV. Desde entonces, observatorios ubicados en Haverah Park del Reino Unido, Yakutsk de Rusia, AGASA de Japón y Dugway de EE.UU. también han observado ECC con energías mayores que 1020 eV. Poco se sabe de dichos rayos, y en particular cuál es la naturaleza del primario, de dónde provienen, y cómo son acelerados, pero su naturaleza ultrarelativista excluye la mayoría de las respuestas dejando sólo algunas plausibles de ser investigadas experimentalmente. Grupos de científicos de 20 países están trabajando con el fin de construir dos arreglos de detectores gigantes, uno en cada hemisferio a lo largo de 3000 km2 c/u. Dichas dimensiones son necesarias debido al flujo estimado de 1 rayo cósmico/centuria/km2/sr. La sede del Observatorio del Sur es la Argentina. El proyecto fue nombrado Pierre Auger en conmemoración del célebre físico francés que detectó por primera vez chubascos cósmicos en 1938. El proyecto focaliza su interés en rayos cósmicos con energías mayores que 1020 eV.

A Method to Search for Correlations of Ultra-high Energy Cosmic-Ray Masses with the Large-scale Structures in the Local Galaxy Density Field

NASA Astrophysics Data System (ADS)

Ivanov, A. A.

2013-02-01

One of the main goals of investigations using present and future giant extensive air shower (EAS) arrays is the mass composition of ultra-high energy cosmic rays (UHECRs). A new approach to the problem is presented, combining the analysis of arrival directions with the statistical test of the paired EAS samples. One of the ideas of the method is to search for possible correlations between UHECR masses and their separate sources; for instance, if there are two sources in different areas of the celestial sphere injecting different nuclei, but the fluxes are comparable so that arrival directions are isotropic, then the aim is to reveal a difference in the mass composition of cosmic-ray fluxes. The method is based on a non-parametric statistical test—the Wilcoxon signed-rank routine—which does not depend on the populations fitting any parameterized distributions. Two particular algorithms are proposed: first, using measurements of the depth of the EAS maximum position in the atmosphere; and second, relying on the age variance of air showers initiated by different primary particles. The formulated method is applied to the Yakutsk array data, in order to demonstrate the possibility of searching for a difference in average mass composition between the two UHECR sets, arriving particularly from the supergalactic plane and a complementary region.

The organizations for space education and outreach programs in the Republic of Korea

NASA Astrophysics Data System (ADS)

Lee, Jeongwon; Jo, Hyun-Jung; Choi, Jae Dong

2011-09-01

Korea has a short history in space development compared to neighboring countries like Japan, China, India and Russia. During the past 20 years, Korea has focused on developing satellite and rocket space technology under the national space development plan. KOMPSAT-1 and 2, and KSLV-1 are the results of the selection and concentration policy of the Korean government. Due to the arduous mission of developing hardware oriented space technology, the topic of space education and outreach for the general public has not received much in the national space program. But recently, the Korean government has begun planning a space science outreach program in the detailed action plan of the mid-long term national space development plan. This paper introduces and analyzes the organizations performing space education and outreach programs for primary and secondary schools in the Republic of Korea. "Young Astronaut Korea (YAK)" is one such program. This is a non-profit organization established to provide space education for students in 1989 when Korea just started its space development program. "YAK" is a unique group in Korea for space education and outreach activities because it is organized by branches at each school in the nation and it is much like the Boy Scout and Girl Scout programs. Space Science Museum and National Youth Space Center (NYSC), which are located near NARO space center in the southernmost part of the Korean peninsula are other examples of space education and outreach programs. NARO space center, which is the only launch site in Korea became the center of public interest by showing the KSLV-1 launch in 2009 and will be expected to play a key role for the space education of students in the Republic of Korea. The NYSC will perform many mission oriented space education programs for students as Space Camp in the USA does. This paper introduces the status of the space education and outreach programs of each organization and presents the future direction of space education and outreach for the Korean public and students. If these three organizations cooperate with each other and develop systematic programs of space education and outreach for the people, they will prepare a base for growth and progress in future space science and technology in Korea.

Screening of Probiotic Activities of Lactobacilli Strains Isolated from Traditional Tibetan Qula, A Raw Yak Milk Cheese

PubMed Central

Zhang, Bei; Wang, Yanping; Tan, Zhongfang; Li, Zongwei; Jiao, Zhen; Huang, Qunce

2016-01-01

In this study, 69 lactobacilli isolated from Tibetan Qula, a raw yak milk cheese, were screened for their potential use as probiotics. The isolates were tested in terms of: Their ability to survive at pH 2.0, pH 3.0, and in the presence of 0.3% bile salts; tolerance of simulated gastric and intestinal juices; antimicrobial activity; sensitivity against 11 specific antibiotics; and their cell surface hydrophobicity. The results show that out of the 69 strains, 29 strains (42%) had survival rates above 90% after 2 h of incubation at pH values of 2.0 or 3.0. Of these 29 strains, 21 strains showed a tolerance for 0.3% bile salt. Incubation of these 21 isolates in simulated gastrointestinal fluid for 3 h revealed survival rates above 90%; the survival rate for 20 of these isolates remained above 90% after 4 h of incubation in simulated intestinal fluid. The viable counts of bacteria after incubation in simulated gastric fluid for 3 h and simulated intestinal fluid for 4 h were both significantly different compared with the counts at 0 h (p<0.001). Further screening performed on the above 20 isolates indicated that all 20 lactobacilli strains exhibited inhibitory activity against Micrococcus luteus ATCC 4698, Bacillus subtilis ATCC 6633, Listeria monocytogenes ATCC 19115, and Salmonella enterica ATCC 43971. Moreover, all of the strains were resistant to vancomycin and streptomycin. Of the 20 strains, three were resistant to all 11 elected antibiotics (ciprofloxacin, erythromycin, tetracycline, penicillin G, ampicillin, streptomycin, polymyxin B, vancomycin, chloramphenicol, rifampicin, and gentamicin) in this study, and five were sensitive to more than half of the antibiotics. Additionally, the cell surface hydrophobicity of seven of the 20 lactobacilli strains was above 70%, including strains Lactobacillus casei 1,133 (92%), Lactobacillus plantarum 1086-1 (82%), Lactobacillus casei 1089 (81%), Lactobacillus casei 1138 (79%), Lactobacillus buchneri 1059 (78%), Lactobacillus plantarum1141 (75%), and Lactobacillus plantarum 1197 (71%). Together, these results suggest that these seven strains are good probiotic candidates, and that tolerance against bile acid, simulated gastric and intestinal juices, antimicrobial activity, antibiotic resistance, and cell surface hydrophobicity could be adopted for preliminary screening of potentially probiotic lactobacilli. PMID:26954218

Fiber degradation potential of natural co-cultures of Neocallimastix frontalis and Methanobrevibacter ruminantium isolated from yaks (Bos grunniens) grazing on the Qinghai Tibetan Plateau.

PubMed

Wei, Ya-Qin; Long, Rui-Jun; Yang, Hui; Yang, Hong-Jian; Shen, Xi-Hui; Shi, Rui-Fang; Wang, Zhi-Ye; Du, Jun-Guo; Qi, Xiao-Jin; Ye, Qian-Hong

2016-06-01

Several natural anaerobic fungus-methanogen co-cultures have been isolated from rumen and feces source of herbivores with strong fiber degrading ability. In this study, we isolated 7 Neocallimastix with methanogen co-cultures from the rumen of yaks grazing on the Qinghai Tibetan Plateau. Based on morphological characteristics and internal transcribed spacer 1 sequences (ITS1), all the fungi were identified as Neocallimastix frontalis. The co-cultures were confirmed as the one fungus - one methanogen pattern by the PCR-denatured gradient gel electrophoresis (DGGE) assay. All the methanogens were identified as Methanobrevibacter ruminantium by 16s rRNA gene sequencing. We investigated the biodegrading capacity of the co-culture (N. frontalis + M. ruminantium) Yaktz1 on wheat straw, corn stalk and rice straw in a 7 days-incubation. The in vitro dry matter digestibility (IVDMD), acid detergent fiber digestibility (ADFD) and neural detergent fiber digestibility (NDFD) values of the substrates in the co-culture were significantly higher than those in the mono-culture N. frontalis Yaktz1. The co-culture exhibited high polysaccharide hydrolase (xylanase and FPase) and esterase activities. The xylanase in the co-culture reached the highest activity of 12500 mU/ml on wheat straw at the day 3 of the incubation. At the end of the incubation, 3.00 mmol-3.29 mmol/g dry matter of methane were produced by the co-culture. The co-culture also produced high level of acetate (40.00 mM-45.98 mM) as the end-product during the biodegradation. Interestingly, the N. frontalis Yaktz1 mono-culture produced large amount of lactate (8.27 mM-11.60 mM) and ethanol (163.11 mM-242.14 mM), many times more than those recorded in the previously reported anaerobic fungi. Our data suggests that the (N. frontalis + M. ruminantium) Yaktz1 co-culture and the N. frontalis Yaktz1 mono-culture both have great potentials for different industrial use. Copyright © 2016. Published by Elsevier Ltd.

Sources of black carbon to the Himalayan-Tibetan Plateau glaciers

NASA Astrophysics Data System (ADS)

Li, Chaoliu; Bosch, Carme; Kang, Shichang; Andersson, August; Chen, Pengfei; Zhang, Qianggong; Cong, Zhiyuan; Chen, Bing; Qin, Dahe; Gustafsson, Örjan

2016-08-01

Combustion-derived black carbon (BC) aerosols accelerate glacier melting in the Himalayas and in Tibet (the Third Pole (TP)), thereby limiting the sustainable freshwater supplies for billions of people. However, the sources of BC reaching the TP remain uncertain, hindering both process understanding and efficient mitigation. Here we present the source-diagnostic Δ14C/δ13C compositions of BC isolated from aerosol and snowpit samples in the TP. For the Himalayas, we found equal contributions from fossil fuel (46+/-11%) and biomass (54+/-11%) combustion, consistent with BC source fingerprints from the Indo-Gangetic Plain, whereas BC in the remote northern TP predominantly derives from fossil fuel combustion (66+/-16%), consistent with Chinese sources. The fossil fuel contributions to BC in the snowpits of the inner TP are lower (30+/-10%), implying contributions from internal Tibetan sources (for example, yak dung combustion). Constraints on BC sources facilitate improved modelling of climatic patterns, hydrological effects and provide guidance for effective mitigation actions.

Research Results

NASA Astrophysics Data System (ADS)

2012-01-01

In situ Oxidation Study of Pt (110) and Its Interaction with CO Chinese Scientists Published a Paper on Prevention of Drug Craving and Relapse by Memory Retrieval-extinction Procedure in Science Series Papers Published in Energy Policy: Modeling Energy Use of China's Road Transport and Policy Evaluation Breakthrough in the Ambient Catalytic Destruction of Formaldehyde Novel Findings for High Altitude Adaptation from the Yak Genome Binary Colloidal Structures Assembled through Ising Interactions Reemergence of superconductivity at 48K in Compressed Iron Selenide Based Superconductors Nucleosomes Suppress Spontaneous Mutations Base-Specifically in Eukaryotes Single-Chain Fragmented Antibodies Guided SiRNA Delivery in Breast Cancer Does Yeast Suicide? China Scientists Developed Important Methodologies for Spatiotemporal Detecting and Manipulating of Cellular Activities Scorpions Inspire Chinese Scientists in Making Bionic Non-eroding Surfaces for Machinery Research on Phylogenetic Placement of Borthwickia and Description of a New Family of Angiosperms, Borthwickiaceae Plasmoid Ejection and Secondary Current Sheet Generation from Magnetic Reconnection in Laser-plasma Interaction Cotton Bollworm Adapts to Bt Cotton via Diverse Mutations A Histone Acetyltransferase Regulates Active DNA Demethylation in Arabidopsis

16 KB/S Modem (AN/GSC-38) CONUS Test

DTIC Science & Technology

1980-08-01

1.4E-3 1500 MOS 2.5-4 1400 6 LEE 4.3E-3 1640 B MEM 0 1440 POl 2.5E-3 1860 ARL 5.0-5 1580 TOL I.1E-3 1900 DRA 2.0-5 150 NET 3.5E-3 2000 SEG 1.1-4 19yno C...LAM-POL 1.6-3 0.60 0.40 F’I,-LAM 1.2-4 0.63 0.37 LAM- MEM 4.8-3 0.53 0.47 SOC-POL j.,1-4 0.50 0.50 LAM-ORE 7.8-3 O.G2 0.38 SOC-YAK 1.4-3 0.65 0.35 MOU...0.61 0.39 NBD-HEL 4.5-4 0.84 0.16 NBD-CMC 3.2 4 1.0 0 NBD-LAM 7.8-3 0.25 0.75 NBD--MOU 2.7-3 0.48 0.52 NBD- MEM 5.6-3 0.14 0.86 NBD-JAS 5.3-3 0.69

Whole-genome resequencing reveals world-wide ancestry and adaptive introgression events of domesticated cattle in East Asia.

PubMed

Chen, Ningbo; Cai, Yudong; Chen, Qiuming; Li, Ran; Wang, Kun; Huang, Yongzhen; Hu, Songmei; Huang, Shisheng; Zhang, Hucai; Zheng, Zhuqing; Song, Weining; Ma, Zhijie; Ma, Yun; Dang, Ruihua; Zhang, Zijing; Xu, Lei; Jia, Yutang; Liu, Shanzhai; Yue, Xiangpeng; Deng, Weidong; Zhang, Xiaoming; Sun, Zhouyong; Lan, Xianyong; Han, Jianlin; Chen, Hong; Bradley, Daniel G; Jiang, Yu; Lei, Chuzhao

2018-06-14

Cattle domestication and the complex histories of East Asian cattle breeds warrant further investigation. Through analysing the genomes of 49 modern breeds and eight East Asian ancient samples, worldwide cattle are consistently classified into five continental groups based on Y-chromosome haplotypes and autosomal variants. We find that East Asian cattle populations are mainly composed of three distinct ancestries, including an earlier East Asian taurine ancestry that reached China at least ~3.9 kya, a later introduced Eurasian taurine ancestry, and a novel Chinese indicine ancestry that diverged from Indian indicine approximately 36.6-49.6 kya. We also report historic introgression events that helped domestic cattle from southern China and the Tibetan Plateau achieve rapid adaptation by acquiring ~2.93% and ~1.22% of their genomes from banteng and yak, respectively. Our findings provide new insights into the evolutionary history of cattle and the importance of introgression in adaptation of cattle to new environmental challenges in East Asia.

Composition of the spring Siberian troposphere during YAK-AEROSIB 2010: Influence of biomass burning, stratospheric intrusion and the Eyjafjöll eruption

NASA Astrophysics Data System (ADS)

Paris, J.; Berchet, A.; Arshinov, M.; Nedelec, P.; Stohl, A.; Ancellet, G.; Law, K.; Belan, B. D.; Ramonet, M.; Ciais, P.

2010-12-01

Despite their potential relevance to atmospheric environment issues, measurements in Siberia, in the outflow of Europe and upstream of Eastern Asia and the Arctic, are sparse. Impacts of remote and regional sources on this air shed’s composition remain virtually unexplored. We document the tropospheric composition and address these issues using CO2, CO, O3 and novel CH4 measurement data from the 2010 YAK-AEROSIB intensive airborne campaign over Siberia. The campaign took place in April, spring being highly relevant here because of longer lifetime of pollutants, transported especially to the Arctic. We analysed the data by comparing trace gases concentrations enhancements, FLEXPART simulations of backward transport and by comparison with other campaigns. We illustrate our findings by three case studies. Typical observed mixing ratios of trace gases were 394 ppm CO2, 140 ppb CO, 59 ppb O3 and 1843 ppb CH4 across the campaign. CO2 increased by ~4 ppm relative to a similar 2006 spring campaign (Paris et al., 2010), a slightly slower pace than the hemispheric increase of 1.75 ppm/yr (as estimated over the period 2006-2008). This difference could be due to different transport pattern, with more air from the Arctic, and hence less affected by pollution, than in 2006; vegetation respiration is low in largely snow-covered Siberia. CO mixing ratio is also ~5 ppb lower than our 2006 spring values, possibly also explained by a pervasive airmass of Arctic origin in 2010. But large enhancements of CO and other trace gases have been observed. CO enhancements were connected to biomass burning through tracer correlation and FLEXPART simulations convolved with fire emissions. We find that contributions from fires in agricultural areas in Western Russia and Northern Kazakhstan influence significantly CO concentrations over Siberia (+50 ppb) strongly correlated (r=0.86) to an O3 enhancement of ~+5 ppb (regression slope 2.5 ppb/ppb). O3 concentrations as high as 210 ppb were observed during flight from Bratsk to Novosibirsk, simultaneously with lower CO and CO2 concentrations. This high concentration occurred at 6 km altitude immediately before crossing a cold front and are interpreted as a stratospheric dry intrusion in the troposphere. We use this event to discuss the diffusivity of Flexpart in the analysis of such events. Finally, a consistent strong O3 depletion at 6 km altitude over Siberia is tentatively attributed to the Eyjafjoll eruption based on FLEXPART backward simulation.

Interplay between climatic and tectonic processes in the St. Elias foreland, southern Alaska: Evolution of a glaciated convergent margin since the mid-Pleistocene

NASA Astrophysics Data System (ADS)

Worthington, L. L.; Gulick, S. P.; Ridgway, K. R.; Jaeger, J. M.; Cowan, E. A.; Slagle, A. L.; Forwick, M.

2013-12-01

The offshore St. Elias fold-thrust belt records the complex interaction between collisional tectonics and glacial climate variability, providing insight for models of orogenesis and the evolution of glacial depocenters. Ongoing collision of the Yakutat (YAK) microplate with North America (NA) has driven orogenesis of the St. Elias Mountains and the advance of the offshore deformation front to the southeast. Glacial erosion and deposition have provided sediment that constructed the upper continental shelf, much of which has been reincorporated into the orogenic wedge through offshore faulting and folding. We integrate core and downhole logging data from IODP Expedition 341 (Sites U1420 and U1421) drilled on the Yakutat shelf and slope with high-resolution and regional seismic profiles to investigate the coupled structural and stratigraphic evolution of the St. Elias margin. Site U1420 lies on the Yakutat shelf within the Bering Trough, a shelf-crossing trough that is within primary depocenter for Bering Glacier sediments. The sub-seafloor architecture of the Bering Trough region is defined by a regional unconformity that marks the first glacial advance to the shelf edge. Below the unconformity, the shelf is constructed by multiple aggradational packages that are likely a series of pro-glacial outer shelf/slope fans. Two faults underlie the glacial packages and have been rendered inactive as the depositional environment has evolved, while faulting elsewhere on the shelf has initiated. Site U1421 lies on the current continental slope, within the backlimb of an active thrust that forms part of the modern YAK-NA deformation front. At each of these sites, we recovered glacigenic diamict (at depths up to ~1015 m at Site U1420), all of which is younger than 0.781 Ma. Preliminary age models for the Bering Trough region indicate that the entire outer shelf and shelf edge environment have been built since the Mid-Pleistocene Transition (MPT), and is possibly even younger. In stark contrast to previous interpretations, the shelf environment, in addition to the proximal deep-sea fan system, appears to be a primary glacial depocenter since the MPT, with an average accumulation rate >1.3 mm/yr. Additionally, initiation of active deformation away from the Bering Trough depocenter likely occurred since ~1 Ma. These observations suggest that possible tectonic reorganization due to mass redistribution by glacial processes occurs at time scales on the order of 100kyr-1Myr. It follows that the St. Elias orogenic system may be more sensitive to glacial-interglacial cycles than previously recognized.

Holocene glacier fluctuations and migration of Neolithic yak pastoralists into the high valleys of northwest Bhutan

NASA Astrophysics Data System (ADS)

Meyer, M. C.; Hofmann, Ch.-Ch.; Gemmell, A. M. D.; Haslinger, E.; Häusler, H.; Wangda, D.

2009-06-01

Here we present geomorphologic, palaeoenvironmental and archaeo-botanical data which elucidate the Late Pleistocene and Holocene glacial history of the high, mountain-locked Himalayan valleys in northwest Bhutan and provide one of the earliest proofs of human activity yet known for the High Himalaya range. In this area, difficult to access, close linkage between climatic change, glacier fluctuations and human migration patterns has been discovered. Glacier systems in the studied area are characterized by avalanching and debris mantled glacier snouts, with the significant local influence of the Indian summer monsoon causing decoupling of glacier responses from temperature changes but supporting the idea of monsoonal forcing. Geomorphologic mapping, together with Optically Stimulated Luminescence (OSL) and radiocarbon dating of ice-proximal sediments, has been used to construct a local glacial chronology. Local ice-stream networks developed during the Early Holocene (ca 10,000-9000 a ago) and during the early part of the Mid Holocene (6710 ± 90-4680 ± 155 cal a BP) at which times there were ice advances of about 5 km from the modern glacier termini. At such times, the intensity of pro- and periglacial processes would have intensified and ice-dammed lakes were probably common as well, rendering human colonization of the high valleys in northwest Bhutan impossible. An abrupt shift to dry climatic conditions on the Tibetan Plateau between 5000 and 4500 a BP coincided with glacial decay and the onset of morphodynamically stable conditions on the broad valley floors of the high valleys in this part of the Himalaya. Palynological data suggest that the sudden disappearance of juniper and rhododendron pollen, the immediate onset of pollen input from cereals (confirmed by detailed SEM analysis) and a clear pattern of over-grazing, trampling and peat deterioration can be linked to human arrival in the valleys at ca 4280 ± 130 cal a BP. Extensive charcoal horizons dating to 4745 ± 250 and 4680 ± 155 cal a BP are interpreted as evidence for human use of fire and forest clearances and agree spatially and temporally with the pollen-based picture. Charcoal occurrences as old as 6710 ± 90 cal a BP might be linked to yet earlier exploration of these Himalayan valleys during phases of low glacial activity. We provide an account of the colonization of these high valleys in response to glacial and monsoonal change and argue that the most likely founder societies come from the Tibetan Plateau, where yak and barley based pastoralism and Neolithic settlements are known to have existed since the Mid Holocene.

In-Situ Observations of a Subglacial Outflow Plume in a Greenland Fjord

NASA Astrophysics Data System (ADS)

Mankoff, K. D.; Straneo, F.; Singh, H.; Das, S. B.

2014-12-01

We present oceanographic observations collected in and immediately outside of a buoyant, fresh, sediment-laden subglacial outflow plume rising up the marine-terminating front of Sarqardleq Glacier, Greenland (68.9 N, 50.4 W). Subglacial outflow plumes, associated with the discharge at depth of upstream glacial surface melt, entrain the relatively warm fjord waters and are correlated with enhanced submarine melt and increased calving. Few in-situ observations exist due to the challenges of making measurements at the calving front of glaciers. Our data were collected using a small boat, a helicopter, and a JetYak (a remote-controlled jet-ski-powered kayak). Temperature and salinity profiles in, around, and far from the plume are used to described its oceanographic properties, spatial extent, and temporal variability. This plume rises vertically up the ice front expanding laterally and away from the ice, over-shoots its stable isopycnal and reaches the surface. Its surface expression is identified by colder, saltier, sediment-laden water flowing at ~5 m/s away from the ice face. Within ~300 m from the ice it submerges as it seeks buoyant stability.

Population Genetic Analysis of Theileria annulata from Six Geographical Regions in China, Determined on the Basis of Micro- and Mini-satellite Markers

PubMed Central

Yin, Fangyuan; Liu, Zhijie; Liu, Junlong; Liu, Aihong; Salih, Diaeldin A.; Li, Youquan; Liu, Guangyuan; Luo, Jianxun; Guan, Guiquan; Yin, Hong

2018-01-01

Theileria annulata, a tick-borne apicomplexan protozoan, causes a lymphoproliferative disease of cattle with high prevalence in tropical and sub-tropical regions. Understanding the genetic diversity and structure of local populations will provide more fundamental knowledge for the population genetics and epidemics of protozoa. In this study, 78 samples of T. annulata collected from cattle/yaks representing 6 different geographic populations in China were genotyped using eight micro- and mini-satellite markers. High genetic variation within population, moderate genetic differentiation, and high level of diversity co-occurring with significant linkage disequilibrium were observed, which indicates there is gene flow between these populations in spite of the existence of reproductive and geographical barriers among populations. Furthermore, some degree of genetic differentiation was also found between samples from China and Oman. These findings provide a first glimpse of the genetic diversity of the T. annulata populations in China, and might contribute to the knowledge of distribution, dynamics, and epidemiology of T. annulata populations and optimize the management strategies for control. PMID:29515624

Review of the 2012 Epizootic Hemorrhagic Disease Outbreak in Domestic Ruminants in the United States

PubMed Central

Stevens, G.; McCluskey, B.; King, A.; O’Hearn, E.; Mayr, G.

2015-01-01

An unusually large number of cases of Epizootic hemorrhagic disease (EHD) were observed in United States cattle and white-tailed deer in the summer and fall of 2012. USDA APHIS Veterinary Services area offices were asked to report on foreign animal disease investigations and state diagnostic laboratory submissions which resulted in a diagnosis of EHD based on positive PCR results. EHD was reported in the following species: cattle (129 herds), captive white-tailed deer (65 herds), bison (8 herds), yak (6 herds), elk (1 herd), and sheep (1 flock). A majority of the cases in cattle and bison were found in Nebraska, South Dakota, and Iowa. The majority of cases in captive white-tailed deer were found in Ohio, Iowa, Michigan, and Missouri. The most common clinical sign observed in the cattle and bison herds was oral lesions. The major observation in captive white-tailed deer herds was death. Average within-herd morbidity was 7% in cattle and bison herds, and 46% in captive white-tailed deer herds. The average within-herd mortality in captive white-tailed deer herds was 42%. PMID:26244773

Quantitative Structure-Activity Relationship Modeling Coupled with Molecular Docking Analysis in Screening of Angiotensin I-Converting Enzyme Inhibitory Peptides from Qula Casein Hydrolysates Obtained by Two-Enzyme Combination Hydrolysis.

PubMed

Lin, Kai; Zhang, Lanwei; Han, Xue; Meng, Zhaoxu; Zhang, Jianming; Wu, Yifan; Cheng, Dayou

2018-03-28

In this study, Qula casein derived from yak milk casein was hydrolyzed using a two-enzyme combination approach, and high angiotensin I-converting enzyme (ACE) inhibitory activity peptides were screened by quantitative structure-activity relationship (QSAR) modeling integrated with molecular docking analysis. Hydrolysates (<3 kDa) derived from combinations of thermolysin + alcalase and thermolysin + proteinase K demonstrated high ACE inhibitory activities. Peptide sequences in hydrolysates derived from these two combinations were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). On the basis of the QSAR modeling prediction, a total of 16 peptides were selected for molecular docking analysis. The docking study revealed that four of the peptides (KFPQY, MPFPKYP, MFPPQ, and QWQVL) bound the active site of ACE. These four novel peptides were chemically synthesized, and their IC 50 was determined. Among these peptides, KFPQY showed the highest ACE inhibitory activity (IC 50 = 12.37 ± 0.43 μM). Our study indicated that Qula casein presents an excellent source to produce ACE inhibitory peptides.

Investigating the Human Dimension of Unprecedented Global Climate Change in northeastern Siberia, Russia: Understandings, Perceptions and Responses

NASA Astrophysics Data System (ADS)

Crate, S.

2009-12-01

An urgent challenge of the 21st century is to investigate understandings, perceptions and responses of populations confronting the local effects of global climate change. This paper describes the most recent results of one such project working with rural native Viliui Sakha communities, Turkic-speaking horse & cattle breeders in northeastern Siberia, Russia. The research questions are: 1) What local effects of global climate change are Viliui Sakha communities observing, how are Viliui Sakha perceiving these changes and how are the changes affecting both their subsistence survival and their cultural orientations? 2) What local knowledge exists about past climate perturbations and how does that knowledge influence contemporary adaptation to global climate change? 3) How can anecdotal (local) knowledge and regional scientific knowledge about the local effects of global climate change be integrated to enhance both local adaptive responses and policy efforts? The four-village, three-year study is a collaborative effort involving the active participation of the targeted communities, field assistants, native specialists, an in-country research team and an international collaborator. The project is founded on the PI’s 20 years of ongoing research and work with rural Viliui Sakha communities and on her fluency in both the Sakha and Russian languages. A central focus of this project is the integration of local and scientific knowledges. We are documenting local knowledge on the community, elder and archival levels. We are collaborating with scientists in Yakutsk for regional scientific data. Our project team has just returned from the second summer of field work and this presentation will cover the project results to date. Hayfields are inundated with water.

Investigation of the structure and dynamics of the ozone layer in the Eastern Arctic region during EASOE Campaign

NASA Technical Reports Server (NTRS)

Khattatov, V.; Yushkov, V.; Rudakov, V.; Zaitsev, I.; Rosen, J.; Kjome, N.

1994-01-01

Balloon measurements of the vertical distribution of ozone and aerosol were made at Dickson Island (73 deg N, 81 deg E), Kiruna (68 deg N, 20 deg E) and Heiss Island (81 deg N, 58 deg E) from December 1991 to March 1992. To acquire data on the seasonal variability of the vertical ozone distribution, electrochemical ozonesondes ECC-4A were flown three times a week. With ozonesondes on the same balloons, backscattersondes were flown on the average of two or three times per month. Using these instruments, altitude profiles of backscatter ratio were measured at two wavelengths centered at 490 nm and 940 nm. Additionally, at Heiss Island, Dickson, and Yakutsk (63 deg N, 130 deg E) regular total ozone measurements were obtained using Brewer spectrophotometers. Based on measurements of backscatter ratio it was found that after the Pinatubo eruption in June 1991 significant amount of stratospheric aerosols were formed and transported to the Arctic before the polar vortex was well developed. Analysis of ozone data has shown a deep decrease of ozone concentration in the lower stratosphere in times of intensive transportation of air masses from low latitudes to the polar region in the second half of January and also for some periods in February and March of 1992. When the values of backscatter ratio beta were more than 8-10 at a wavelength of 940 nm strong anticorrelation occurred between aerosol loading and ozone concentration in the lower stratosphere. At 50-70 deg N, the mean monthly values of total ozone in winter-spring 1992 proved to be much lower than the climatic mean values.

Chronic Respiratory Diseases in the Regions of Northern Russia: Epidemiological Distinctions in the Results of a National Population Study.

PubMed

Gambaryan, Marine H; Shalnova, Svetlana A; Deev, Alexander D; Drapkina, Oxana M

2017-07-26

The aim of the study is to investigate the epidemiological situation regarding chronic respiratory diseases in populations that inhabit different climatic-geographical regions of Russia, and to develop targeted programs for prevention of these diseases. (1) a comparative analysis of the standardized mortality data in Russia and other selected regions of the Russian North using the European standard for respiratory diseases, in a population aged 25-64; and (2) data from a randomized cross-sectional epidemiological study, with subjects from three different climatic-geographical regions of Russia. (1) the respiratory disease-related mortality rates in the majority of Russian Northern regions were much higher compared to the national average. Although death rates from chronic lower respiratory diseases were higher among the Northern regions and in the whole of Russia relative to the countries of European Union (EU), the cause of death in the populations of the Northern regions tend to be lower respiratory infections and pneumonia; and (2) despite the absence of any significant differences in the prevalence of smoking, the prevalence of chronic respiratory diseases (COPD) is significantly higher in Far North Yakutsk compared to the other two regions in this study-Chelyabinsk and Vologda. The status of hyperborean had the highest chance of a significant contribution to COPD and cardiorespiratory pathology among all other risk factors. The results revealed a need for effective targeted strategies for primary and secondary prevention of chronic respiratory diseases for the populations of the Northern regions of Russia. The revealed regional distinctions regarding the prevalence of, and mortality from, chronic respiratory diseases should be taken into consideration when designing integrated programs for chronic non-communicable disease prevention in these regions.

Pyrosequencing analysis of the microbial diversity of airag, khoormog and tarag, traditional fermented dairy products of mongolia.

PubMed

Oki, Kaihei; Dugersuren, Jamyan; Demberel, Shirchin; Watanabe, Koichi

2014-01-01

Here, we used pyrosequencing to obtain a detailed analysis of the microbial diversities of traditional fermented dairy products of Mongolia. From 22 Airag (fermented mare's milk), 5 Khoormog (fermented camel's milk) and 26 Tarag (fermented milk of cows, goats and yaks) samples collected in the Mongolian provinces of Arhangai, Bulgan, Dundgobi, Tov, Uburhangai and Umnugobi, we obtained a total of 81 operational taxonomic units, which were assigned to 15 families, 21 genera and 41 species in 3 phyla. The genus Lactobacillus is a core bacterial component of Mongolian fermented milks, and Lactobacillus helveticus, Lactobacillus kefiranofaciens and Lactobacillus delbrueckii were the predominant species of lactic acid bacteria (LAB) in the Airag, Khoormog and Tarag samples, respectively. By using this pyrosequencing approach, we successfully detected most LAB species that have been isolated as well as seven LAB species that have not been found in our previous culture-based study. A subsequent analysis of the principal components of the samples revealed that L. delbrueckii, L. helveticus, L. kefiranofaciens and Streptococcus thermophilus were the main factors influencing the microbial diversity of these Mongolian traditional fermented dairy products and that this diversity correlated with the animal species from which the milk was sourced.

Habitat Variability and Ethnic Diversity in Northern Tibetan Plateau.

PubMed

Jia, Xin; Lee, Harry F; Cui, Mengchun; Liu, Chao; Zeng, Lin; Yue, Ricci P H; Zhao, Yang; Lu, Huayu

2017-04-20

There are 56 officially-recognized ethnic groups in China. However, the distinct geographic patterns of various ethnic groups in relation to the physical environment in China have rarely been investigated. Based on the geo-referenced physical environmental parameters of 455 Han, Tu, Hui, Salar, Mongolian, and Tibetan communities in Qinghai, we found that the communities could be statistically demarcated by temperature and aridity threshold according to their ethnicity, implying that the geographic distribution of each ethnic group is mediated by the physical environment. We also observed that the habitat of each ethnic group is ecologically compatible with current subsistence strategies. Tibetans settle in cold and humid high-altitude regions owing to the cultivation of highland barley and the breeding of yak, dzo, Tibetan sheep and Tibetan goat. Mongolians survive by animal husbandry in cold and dry grassland areas. Han and Tu people settle in the Huangshui River Valley, which offers relatively humid climate and flat land for agriculture. Hui and Salar people occupy the Yellow River Valley with its relatively arid environment and grassland vegetation suitable for animal breeding. Our findings offer a new perspective in explaining the geographic patterns and the varieties of ethnic groups in China and elsewhere.

Thermodynamic properties of the Group 1A elements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Alcock, C.B.; Itkin, V.P.; Chase, M.W.

1994-05-01

This review describes thermodynamic properties of condensed phases of the alkali metals, excluding francium for which the amount of information is too limited. The properties considered are: heat capacities from 0 to 1600 K, temperatures and enthalpies of fusion and martensitic transformation in Li and Na; discussion of the Debye temperature and electronic heat capacity coefficient at absolute zero temperature is also included. The paper is the second part of a series. Similar to previous assessment of the IIA group [93ALC/CHA], this paper considers original studies, especially with respect to factors which influence the accuracy and reliability of results. Recommendationsmore » derived from such analyses are compared with most advanced previous reviews made at the Institute for High Temperatures (Moscow) [70SHP/YAK], [82GUR] and the National Institute of Standards and Technology (Washington) [85JAN]. The properties of individual elements of the group are compared and suggestions are made for experimental studies which should improve poorly measured quantities. The review is supplemented by an IBM PC database which contains references, assessed data, brief description of studies and has facilities for fitting and plotting of data and for adding new information.« less

Preliminary raptor surveys in western Mongolia

USGS Publications Warehouse

Ellis, D.H.; Ellis, Merlin H.; Tsengeg, Pu

1995-01-01

Raptors were observed on a 5200 km expedition from Ulaan Baatar through the Hangay Mountains to the Russian Altay Mountains with return through the Gobi Altay Mountains. The focus of the expedition was on nesting ecology of the Saker (Falco cherrug) and Altay falcons (F. altaicus) (25 eyries were located), but nests were also found for seven other species including more than 30 nests found of the upland buzzard (Buteo hemilasius). We conducted 21 day-long counts and 10 more formal raptor road counts. Raptors were concentrated in areas where perches were common and where food was most abundant. Western Mongolia remains a vast undeveloped land where camel trains and yak carts are normal. No developed highway network exists. Raptor work in the interior must be supported by four-wheel drive vehicles traveling cross country. Food and fuel for a research team are difficult to procure, but raptor populations are largely unexploited. Raptors frequently nest on the ground or on very low cliffs and tress, and often nest in close proximity of pairs of their own and other species. Several areas of special significance to raptors are discussed including the Taleen Ulaan (Red Steppe) areas of granite dells which we recommend for status as an international reserve.

Methanogen Diversity in Indigenous and Introduced Ruminant Species on the Tibetan Plateau

PubMed Central

Huang, Xiao Dan; Martinez-Fernandez, Gonzalo; Padmanabha, Jagadish; Long, Ruijun; Denman, Stuart E.; McSweeney, Christopher S.

2016-01-01

Host factors are regarded as important in shaping the archaeal community in the rumen but few controlled studies have been performed to demonstrate this across host species under the same environmental conditions. A study was designed to investigate the structure of the methanogen community in the rumen of two indigenous (yak and Tibetan sheep) and two introduced domestic ruminant (cattle and crossbred sheep) species raised and fed under similar conditions on the high altitude Tibetan Plateau. The methylotrophic Methanomassiliicoccaceae was the predominant archaeal group in all animals even though Methanobrevibacter are usually present in greater abundance in ruminants globally. Furthermore, within the Methanomassiliicoccaceae family members from Mmc. group 10 and Mmc. group 4 were dominant in Tibetan Plateau ruminants compared to Mmc. group 12 found to be highest in other ruminants studied. Small ruminants presented the highest number of sequences that belonged to Methanomassiliicoccaceae compared to the larger ruminants. Although the methanogen community structure was different among the ruminant species, there were striking similarities between the animals in this environment. This indicates that factors such as the extreme environmental conditions and diet on the Tibetan Plateau might have a greater impact on rumen methanogen community compared to host differences. PMID:27274707

Integral Model of Noise of an Engine-Propeller Power Plant

NASA Astrophysics Data System (ADS)

Moshkov, P. A.; Samokhin, V. F.

2018-03-01

A semiempirical model is proposed for estimation of the noise levels produced by aircraft piston power plants in the far acoustic field, which takes account of the main sources of noise. The acoustic field is considered as a superposition of fields formed by the radiations from a propeller and a piston engine. For calculation estimation of the levels of tonal noise of the propeller, it is proposed that a semiempirical method developed by the authors earlier be used. To determine the levels of vortex propeller noise which is presumably dominant in the broad-band noise of tractor propellers, it is proposed that one analytical model of trailing-edge noise be used. An empirical model of noise is proposed for calculation of the acoustic characteristics of a piston engine. Good agreement is shown between calculated and experimental data on the noise of power plants with tractor propellers. The data have been derived in acoustic testing of light aircraft of the An-2, Yak-18T, MAI-223M, and F30 type under static conditions at the aviabase of the Moscow Aviation Institute. Lines of further research are formulated for improvement of this procedure and expansion of the field of its application.

Integral Model of Noise of an Engine-Propeller Power Plant

NASA Astrophysics Data System (ADS)

Moshkov, P. A.; Samokhin, V. F.

2018-05-01

A semiempirical model is proposed for estimation of the noise levels produced by aircraft piston power plants in the far acoustic field, which takes account of the main sources of noise. The acoustic field is considered as a superposition of fields formed by the radiations from a propeller and a piston engine. For calculation estimation of the levels of tonal noise of the propeller, it is proposed that a semiempirical method developed by the authors earlier be used. To determine the levels of vortex propeller noise which is presumably dominant in the broad-band noise of tractor propellers, it is proposed that one analytical model of trailing-edge noise be used. An empirical model of noise is proposed for calculation of the acoustic characteristics of a piston engine. Good agreement is shown between calculated and experimental data on the noise of power plants with tractor propellers. The data have been derived in acoustic testing of light aircraft of the An-2, Yak-18T, MAI-223M, and F30 type under static conditions at the aviabase of the Moscow Aviation Institute. Lines of further research are formulated for improvement of this procedure and expansion of the field of its application.

Habitat Variability and Ethnic Diversity in Northern Tibetan Plateau

NASA Astrophysics Data System (ADS)

Jia, Xin; Lee, Harry F.; Cui, Mengchun; Liu, Chao; Zeng, Lin; Yue, Ricci P. H.; Zhao, Yang; Lu, Huayu

2017-04-01

There are 56 officially-recognized ethnic groups in China. However, the distinct geographic patterns of various ethnic groups in relation to the physical environment in China have rarely been investigated. Based on the geo-referenced physical environmental parameters of 455 Han, Tu, Hui, Salar, Mongolian, and Tibetan communities in Qinghai, we found that the communities could be statistically demarcated by temperature and aridity threshold according to their ethnic populations, implying that the geographic distribution of each ethnic group is mediated by the physical environment. We also observed that the habitat of each ethnic group is ecologically compatible with current subsistence strategies. Tibetans settle in cold high-altitude regions owing to the cultivation of highland barley and the breeding of yak, dzo, Tibetan sheep and Tibetan goat. Mongolians survive by animal husbandry in cold and humid grassland areas. The Han and Tu ethnic groups settle in the Huangshui River Valley, which offers relatively humid climate and flat land for agriculture. The Hui and Salar ethnic groups occupy the Yellow River Valley with its relatively arid environment and grassland vegetation suitable for animal breeding. Our findings offer a new perspective in explaining the geographic pattern and the variety of ethnic groups in China and elsewhere.

Production and Structural Characterization of Lactobacillus helveticus Derived Biosurfactant

PubMed Central

Sharma, Deepansh; Saharan, Baljeet Singh; Chauhan, Nikhil; Bansal, Anshul; Procha, Suresh

2014-01-01

A probiotic strain of lactobacilli was isolated from traditional soft Churpi cheese of Yak milk and found positive for biosurfactant production. Lactobacilli reduced the surface tension of phosphate buffer saline (PBS) from 72.0 to 39.5 mNm−1 pH 7.2 and its critical micelle concentration (CMC) was found to be 2.5 mg mL−1. Low cost production of Lactobacilli derived biosurfactant was carried out at lab scale fermenter which yields 0.8 mg mL−1 biosurfactant. The biosurfactant was found least phytotoxic and cytotoxic as compared to the rhamnolipid and sodium dodecyl sulphate (SDS) at different concentration. Structural attributes of biosurfactant were determined by FTIR, NMR (1H and 13C), UPLC-MS, and fatty acid analysis by GCMS which confirmed the presence of glycolipid type of biosurfactant closely similar to xylolipids. Biosurfactant is mainly constituted by lipid and sugar fractions. The present study outcomes provide valuable information on structural characterization of the biosurfactant produced by L. helveticus MRTL91. These findings are encouraging for the application of Lactobacilli derived biosurfactant as nontoxic surface active agents in the emerging field of biomedical applications. PMID:25506070

Pyrosequencing Analysis of the Microbial Diversity of Airag, Khoormog and Tarag, Traditional Fermented Dairy Products of Mongolia

PubMed Central

OKI, Kaihei; DUGERSUREN, Jamyan; DEMBEREL, Shirchin; WATANABE, Koichi

2014-01-01

Here, we used pyrosequencing to obtain a detailed analysis of the microbial diversities of traditional fermented dairy products of Mongolia. From 22 Airag (fermented mare’s milk), 5 Khoormog (fermented camel’s milk) and 26 Tarag (fermented milk of cows, goats and yaks) samples collected in the Mongolian provinces of Arhangai, Bulgan, Dundgobi, Tov, Uburhangai and Umnugobi, we obtained a total of 81 operational taxonomic units, which were assigned to 15 families, 21 genera and 41 species in 3 phyla. The genus Lactobacillus is a core bacterial component of Mongolian fermented milks, and Lactobacillus helveticus, Lactobacillus kefiranofaciens and Lactobacillus delbrueckii were the predominant species of lactic acid bacteria (LAB) in the Airag, Khoormog and Tarag samples, respectively. By using this pyrosequencing approach, we successfully detected most LAB species that have been isolated as well as seven LAB species that have not been found in our previous culture-based study. A subsequent analysis of the principal components of the samples revealed that L. delbrueckii, L. helveticus, L. kefiranofaciens and Streptococcus thermophilus were the main factors influencing the microbial diversity of these Mongolian traditional fermented dairy products and that this diversity correlated with the animal species from which the milk was sourced. PMID:25003019

Soil bacterial and fungal diversity differently correlated with soil biochemistry in alpine grassland ecosystems in response to environmental changes.

PubMed

Zhang, Yong; Dong, Shikui; Gao, Qingzhu; Liu, Shiliang; Ganjurjav, Hasbagan; Wang, Xuexia; Su, Xukun; Wu, Xiaoyu

2017-03-06

To understand effects of soil microbes on soil biochemistry in alpine grassland ecosystems under environmental changes, we explored relationships between soil microbial diversity and soil total nitrogen, organic carbon, available nitrogen and phosphorus, soil microbial biomass and soil enzyme activities in alpine meadow, alpine steppe and cultivated grassland on the Qinghai-Tibetan plateau under three-year warming, enhanced precipitation and yak overgrazing. Soil total nitrogen, organic carbon and NH 4 -N were little affected by overgrazing, warming or enhanced precipitation in three types of alpine grasslands. Soil microbial biomass carbon and phosphorus along with the sucrase and phosphatase activities were generally stable under different treatments. Soil NO 3 -N, available phosphorus, urease activity and microbial biomass nitrogen were increased by overgrazing in the cultivated grassland. Soil bacterial diversity was positively correlated with, while soil fungal diversity negatively with soil microbial biomass and enzyme activities. Soil bacterial diversity was negatively correlated with, while soil fungal diversity positively with soil available nutrients. Our findings indicated soil bacteria and fungi played different roles in affecting soil nutrients and microbiological activities that might provide an important implication to understand why soil biochemistry was generally stable under environmental changes in alpine grassland ecosystems.

Pollution impacts on Arctic O3 and CO distributions during POLARCAT summer campaign.

NASA Astrophysics Data System (ADS)

Pommier, M.; Law, K. S.; Clerbaux, C.; Turquety, S.; Schlager, H.; Ancellet, G.; Paris, J.-D.; NASA Arctas Data Team

2009-04-01

The Arctic ozone budget is not well quantified and global models fail to reproduce seasonal cycles especially in summertime when anthropogenic and boreal forest fire emissions can contribute. One possible explanation is the underestimation of modelled ozone production in forest fires plumes. Long-range transport of anthropogenic pollution to the Arctic is also not well quantified. This study focuses on analysis of the POLARCAT summer campaign which took place in Kangerlussuaq, Greenland in July 2008. During the campaign different air masses were sampled including clean northerly air, polluted plumes originating from anthropogenic sources in North American and forest fire plumes from Siberia and Canada. Measurements of O3 and CO collected by the ATR-42 aircraft as part of POLARCAT-France and the German DLR-Falcon aircraft as part of POLARCAT-GRACE are compared to satellite observations from the IASI (Infrared Atmospheric Sounding Interferometer) interferometer. Specific IASI validation flights are also used to validate the measurements. Both in-situ and satellite data are compared to results from the LMDz-INCA global chemistry model. Data from other campaigns such as NASA-ARCTAS and YAK flights in Siberia are also available for these comparisons. Preliminary analyses of Lagrangian matches between aircraft measuring in the same air masses using the CiTTyCAT photochemical trajectory model are presented.

Soil bacterial and fungal diversity differently correlated with soil biochemistry in alpine grassland ecosystems in response to environmental changes

NASA Astrophysics Data System (ADS)

Zhang, Yong; Dong, Shikui; Gao, Qingzhu; Liu, Shiliang; Ganjurjav, Hasbagan; Wang, Xuexia; Su, Xukun; Wu, Xiaoyu

2017-03-01

To understand effects of soil microbes on soil biochemistry in alpine grassland ecosystems under environmental changes, we explored relationships between soil microbial diversity and soil total nitrogen, organic carbon, available nitrogen and phosphorus, soil microbial biomass and soil enzyme activities in alpine meadow, alpine steppe and cultivated grassland on the Qinghai-Tibetan plateau under three-year warming, enhanced precipitation and yak overgrazing. Soil total nitrogen, organic carbon and NH4-N were little affected by overgrazing, warming or enhanced precipitation in three types of alpine grasslands. Soil microbial biomass carbon and phosphorus along with the sucrase and phosphatase activities were generally stable under different treatments. Soil NO3-N, available phosphorus, urease activity and microbial biomass nitrogen were increased by overgrazing in the cultivated grassland. Soil bacterial diversity was positively correlated with, while soil fungal diversity negatively with soil microbial biomass and enzyme activities. Soil bacterial diversity was negatively correlated with, while soil fungal diversity positively with soil available nutrients. Our findings indicated soil bacteria and fungi played different roles in affecting soil nutrients and microbiological activities that might provide an important implication to understand why soil biochemistry was generally stable under environmental changes in alpine grassland ecosystems.

High Milk-Clotting Activity Expressed by the Newly Isolated Paenibacillus spp. Strain BD3526.

PubMed

Hang, Feng; Liu, Peiyi; Wang, Qinbo; Han, Jin; Wu, Zhengjun; Gao, Caixia; Liu, Zhenmin; Zhang, Hao; Chen, Wei

2016-01-12

Paenibacillus spp. BD3526, a bacterium exhibiting a protein hydrolysis circle surrounded with an obvious precipitation zone on skim milk agar, was isolated from raw yak (Bos grunniens) milk collected in Tibet, China. Phylogenetic analysis based on 16S rRNA and whole genome sequence comparison indicated the isolate belong to the genus Paenibacillus. The strain BD3526 demonstrated strong ability to produce protease with milk clotting activity (MCA) in wheat bran broth. The protease with MCA was predominantly accumulated during the late-exponential phase of growth. The proteolytic activity (PA) of the BD3526 protease was 1.33-fold higher than that of the commercial R. miehei coagulant. A maximum MCA (6470 ± 281 SU mL(-1)) of the strain BD3526 was reached under optimal cultivation conditions. The protease with MCA was precipitated from the cultivated supernatant of wheat bran broth with ammonium sulfate and purified by anion-exchange chromatography. The molecular weight of the protease with MCA was determined as 35 kDa by sodium dodecyl sulfate-polyacrylamide gels electrophoresis (SDS-PAGE) and gelatin zymography. The cleavage site of the BD3526 protease with MCA in κ-casein was located at the Met106-Ala107 bond, as determined by mass spectrometry analysis.

Proteomic analysis and cross species comparison of casein fractions from the milk of dairy animals

PubMed Central

Wang, Xiaxia; Zhao, Xiaowei; Huang, Dongwei; Pan, Xiaocheng; Qi, Yunxia; Yang, Yongxin; Zhao, Huiling; Cheng, Guanglong

2017-01-01

Casein micelles contribute to the physicochemical properties of milk and may also influence its functionality. At present, however, there is an incomplete understanding of the casein micelle associated proteins and its diversity among the milk obtained from different species. Therefore, milk samples were collected from seven dairy animals groups, casein fractions were prepared by ultracentrifugation and their constituent proteins were identified by liquid chromatography tandem mass spectrometry. A total of 193 distinct proteins were identified among all the casein micelle preparations. Protein interaction analysis indicated that caseins could interact with major whey proteins, including β-lactoglobulin, α-lactalbumin, lactoferrin, and serum albumin, and then whey proteins interacted with other proteins. Pathway analysis found that the peroxisome proliferator-activated receptor signaling pathway is shared among the studied animals. Additionally, galactose metabolism pathway is also found to be commonly involved for proteins derived from camel and horse milk. According to the similarity of casein micelle proteomes, two major sample clusters were classified into ruminant animals (Holstein and Jersey cows, buffaloes, yaks, and goats) and non-ruminants (camels and horses). Our results provide new insights into the protein profile associated with casein micelles and the functionality of the casein micelle from the studied animals. PMID:28240229

Soil bacterial and fungal diversity differently correlated with soil biochemistry in alpine grassland ecosystems in response to environmental changes

PubMed Central

Zhang, Yong; Dong, Shikui; Gao, Qingzhu; Liu, Shiliang; Ganjurjav, Hasbagan; Wang, Xuexia; Su, Xukun; Wu, Xiaoyu

2017-01-01

To understand effects of soil microbes on soil biochemistry in alpine grassland ecosystems under environmental changes, we explored relationships between soil microbial diversity and soil total nitrogen, organic carbon, available nitrogen and phosphorus, soil microbial biomass and soil enzyme activities in alpine meadow, alpine steppe and cultivated grassland on the Qinghai-Tibetan plateau under three-year warming, enhanced precipitation and yak overgrazing. Soil total nitrogen, organic carbon and NH4-N were little affected by overgrazing, warming or enhanced precipitation in three types of alpine grasslands. Soil microbial biomass carbon and phosphorus along with the sucrase and phosphatase activities were generally stable under different treatments. Soil NO3-N, available phosphorus, urease activity and microbial biomass nitrogen were increased by overgrazing in the cultivated grassland. Soil bacterial diversity was positively correlated with, while soil fungal diversity negatively with soil microbial biomass and enzyme activities. Soil bacterial diversity was negatively correlated with, while soil fungal diversity positively with soil available nutrients. Our findings indicated soil bacteria and fungi played different roles in affecting soil nutrients and microbiological activities that might provide an important implication to understand why soil biochemistry was generally stable under environmental changes in alpine grassland ecosystems. PMID:28262753

From ‘third pole’ to north pole: a Himalayan origin for the arctic fox

PubMed Central

Wang, Xiaoming; Tseng, Zhijie Jack; Li, Qiang; Takeuchi, Gary T.; Xie, Guangpu

2014-01-01

The ‘third pole’ of the world is a fitting metaphor for the Himalayan–Tibetan Plateau, in allusion to its vast frozen terrain, rivalling the Arctic and Antarctic, at high altitude but low latitude. Living Tibetan and arctic mammals share adaptations to freezing temperatures such as long and thick winter fur in arctic muskox and Tibetan yak, and for carnivorans, a more predatory niche. Here, we report, to our knowledge, the first evolutionary link between an Early Pliocene (3.60–5.08 Myr ago) fox, Vulpes qiuzhudingi new species, from the Himalaya (Zanda Basin) and Kunlun Mountain (Kunlun Pass Basin) and the modern arctic fox Vulpes lagopus in the polar region. A highly hypercarnivorous dentition of the new fox bears a striking resemblance to that of V. lagopus and substantially predates the previous oldest records of the arctic fox by 3–4 Myr. The low latitude, high-altitude Tibetan Plateau is separated from the nearest modern arctic fox geographical range by at least 2000 km. The apparent connection between an ancestral high-elevation species and its modern polar descendant is consistent with our ‘Out-of-Tibet’ hypothesis postulating that high-altitude Tibet was a training ground for cold-environment adaptations well before the start of the Ice Age. PMID:24920475

Vulnerability on the Roof of the World: Resilience to Climate Change and Natural Resource Policies on the Tibetan Plateau

NASA Astrophysics Data System (ADS)

Klein, J. A.; Hopping, K. A.; Yeh, E.; Hu, J.; Nyima, Y.; Boone, R.; Galvin, K.; Kang, S.; Ojima, D. S.

2010-12-01

Pastoralists on the Tibetan Plateau are a marginalized people living in an extreme environment and may be especially vulnerable as the system approaches critical thresholds. In Tibet, temperatures are increasing several times more than the global average while the frequency and severity of severe snowstorms is predicted to increase. Pastoralists are also experiencing reduced mobility and severe grazing restrictions. We are using interdisciplinary frameworks and methods that include a multifactor ecological experiment, household interviews, remote sensing, and a coupled ecosystem and household decision-making model to examine herder and ecosystem vulnerability to climate change and extreme weather events within the context of changing natural resource policies in China. The fully factorial ecological experiment includes two climate changes (warming and spring snow additions) and two types of grazing (yak and pika). We established the experiment in 2008 within the Tibet Autonomous Region (4,870 m) and are monitoring microclimate, vegetation, nutrient availability, carbon fluxes and stable isotopes. We are investigating the sensitivity of the system, whether it is likely to cross critical thresholds, and how resilient this system may be to predicted climate and land use changes. Semi-structured interviews on indigenous knowledge and vulnerability complement the ecological experimental work. We are asking herders about climatic and ecological change and vulnerability to snow disasters. To integrate our ecological and social findings, we are coupling an ecosystem model to an agent-based pastoral household model. Our results from the experiment and the indigenous knowledge study suggest that Kobresia pygmaea, the dominant species and primary grazing resource, is vulnerable to warming. Snow additions can partially mediate this effect. Herders throughout this region share common knowledge about both climatic and ecological changes, but appear to be more closely attuned to ecological shifts than to gradual climate changes. Herder perceptions about climate trends often contradict local weather station data, but herders tend to be in strong agreement that grassland health has declined. These results suggest that rangeland degradation has occurred, and that climate warming may be one driver responsible for these changes. While additional snow may improve ecological conditions, the warming-induced degradation may make the social-ecological system more vulnerable to large snowstorm events. Our findings suggest that climate adaptation strategies should address the effects of both warming and extreme weather events and should also encourage land use policies that will maintain these systems under change. The vulnerability of ecosystems on the roof of the world has implications for the 1x109 people living downstream and for feedbacks to the Earth’s climate system.

Thermal State Of Permafrost In Urban Environment Under Changing Climatic Conditions

NASA Astrophysics Data System (ADS)

Streletskiy, D. A.; Grebenets, V. I.; Kerimov, A. G.; Kurchatova, A.; Andruschenko, F.; Gubanov, A.

2015-12-01

Risks and damage, caused by deformation of building and constructions in cryolithozone, are growing for decades. Worsening of cryo-ecological situation and loss of engineering-geocryological safety are induced by both technogenic influences on frozen basement and climate change. In such towns on permafrost as Vorkuta, Dixon more than 60% of objects are deformed, in Yakutsk, Igarka- nearly 40%, in Norilsk, Talnakh, Mirnij 35%, in old indigenous villages - approximately 100%; more than 80% ground dams with frozen cores are in poor condition. This situation is accompanied by activation of dangerous cryogenic processes. For example in growing seasonally-thaw layer is strengthening frost heave of pipeline foundation: only on Yamburg gas condensate field (Taz Peninsula) are damaged by frost heave and cut or completely replaced 3000 - 5000 foundations of gas pipelines. Intensity of negative effects strongly depends on regional geocryology, technogenic loads and climatic trends, and in Arctic we see a temperature rise - warming, which cause permafrost temperature rise and thaw). In built areas heat loads are more diverse: cold foundations (under the buildings with ventilated cellars or near termosyphons) are close to warm areas with technogenic beddings (mainly sandy), that accumulate heat, close to underground collectors for communications, growing thaw zones around, close to storages of snows, etc. Note that towns create specific microclimate with higher air temperature. So towns are powerful technogenic (basically, thermal) presses, placed on permafrost; in cooperation with climate changes (air temperature rise, increase of precipitation) they cause permafrost degradation. The analysis of dozens of urban thermal fields, formed in variable cryological and soil conditions, showed, that nearly 70% have warming trend, 20% - cooling and in 10% of cases the situation after construction is stable. Triggered by warming of climate changes of vegetation, depth and temperature of seasonally thaw layer, summer precipitation regime and other natural parameters in combination with developing technogenesis require new strategy of the cryolithozone development.

Impacts of global warming on boreal larch forest in East Siberia: simulations with a coupled carbon cycle and fire regime model

NASA Astrophysics Data System (ADS)

Ito, A.

2005-12-01

Boreal forest is one of the focal areas in the study of global warming and carbon cycle. In this study, a coupled carbon cycle and fire regime model was developed and applied to a larch forest in East Siberia, near Yakutsk. Fire regime is simulated with a cellular automaton (20 km x 20 km), in which fire ignition, propagation, and extinction are parameterized in a stochastic manner, including the effects of fuel accumulation and weather condition. For each grid, carbon cycle is simulated with a 10-box scheme, in which net biome production by photosynthesis, respiration, decomposition, and biomass burning are calculated explicitly. Model parameters were calibrated with field data of biomass, litter stock, and fire statistics; the carbon cycle scheme was examined with flux measurement data. As a result, the model successfully captured average carbon stocks, productivity, fire frequency, and biomass burning. To assess the effects of global warming, a series of simulations were performed using climatic projections based on the IPCC-SRES emission scenarios from 1990 to 2100. The range of uncertainty among the different climate models and emission scenarios was assessed by using multi-model projection data by CCCma, CCSR/NIES, GFDL, and HCCPR corresponding to the SRES A2 and B2 scenarios. The model simulations showed that global warming in the 21st century would considerably enhance the fire regime (e.g., cumulative burnt area increased by 80 to 120 percent), leading to larger carbon emission by biomass burning. The effect was so strong that growth enhancement by elevated atmospheric CO2 concentration and elongated growing period was cancelled out at landscape scale. In many cases, the larch forest was estimated to act as net carbon sources of 2 to 5 kg C m_|2 by the end of the 21st century, underscoring the importance of forest fire monitoring and management in this region.

RESULTS OF MONITORING METALLO-BETA-LACTAMASE-PRODUCING STRAINS OF PSEUDOMONAS AERUGINOSA IN A MULTI-PROFILE HOSPITAL.

PubMed

Shamaeva, S K; Portnyagina, U S; Edelstein, M V; Kuzmina, A A; Maloguloval, S; Varfolomeeva, N A

2015-01-01

The authors present the results of long-term monitoring of metallo-beta-lactamase (MBL) producing strains of Pseudomonas aeruginosa in the Republican Hospital No 2 of Yakutsk, Russian Federation. Hospitals across Russia, as well as the rest of the world, face a rapid appearance and a virtually unchecked spread of multiresistant and panresistant nosocomial pathogens. Especially prevalent are multidrug-resistant isolates of P. aeruginosa, most often found among the patients of intensive care and intensive therapy units, as well as surgery departments. The aim of this study is to investigate the prevalence of metallo-beta-lactamase-producing strains of P. aeruginosa in a multi-profile hospital. 2,135 isolates of P. aeruginosa were studied, collected during a time span of seven years (2008-2014) from clinical specimens of hospitalised patients in acute surgery, purulent surgery, neurosurgery, otolaryngology, coloproctology departments, intensive care and intensive therapy, burn units, as well as intensive care unit for patients with acute cerebrovascular accidents and coronary care unit. Strains were identified and re-identified using established methods, NEFERMtest 24 (MICROLATEST) biochemical microtest and API (bioMerieux) test systems were used. For all carbapenem-resistant strains a phenotype screening for MBL was performed using the double-disks method with EDTA. In order to identify VIM-type and IMP-type MBL genes a real-time multiplex polymerase chain reaction was used. Among the investigated strains the largest number of P. aeruginosa - 35.6% (761 isolates) was found in patients at intensive care and intensive therapy units. Clonal expansion of extensively drug-resistant strain P. aeruginosa ST235 (VIM-2) was determined, the resistance mechanism of which is connected to MBL. Sensitivity determination of MBL-producing isolates of P. aeruginosa has shown that isolated strains have a high level of resistance (100%) to all tested antibacterial agents: piperacillin, piperacillin-tazobactam, ceftazidime, cefepime, cefoperazone-sulbactam, aztreonam, imipenem, meropenem, doripenem, gentamicin, netilmicin, amikacin, ciprofloxacin, levofloxacin, fosfomicin.

47 CFR 97.221 - Automatically controlled digital station.

Code of Federal Regulations, 2013 CFR

2013-10-01

... station. (a) This rule section does not apply to an auxiliary station, a beacon station, a repeater station, an earth station, a space station, or a space telecommand station. (b) A station may be... 47 Telecommunication 5 2013-10-01 2013-10-01 false Automatically controlled digital station. 97...

47 CFR 97.221 - Automatically controlled digital station.

Code of Federal Regulations, 2012 CFR

2012-10-01

... station. (a) This rule section does not apply to an auxiliary station, a beacon station, a repeater station, an earth station, a space station, or a space telecommand station. (b) A station may be... 47 Telecommunication 5 2012-10-01 2012-10-01 false Automatically controlled digital station. 97...

47 CFR 97.221 - Automatically controlled digital station.

Code of Federal Regulations, 2014 CFR

2014-10-01

... station. (a) This rule section does not apply to an auxiliary station, a beacon station, a repeater station, an earth station, a space station, or a space telecommand station. (b) A station may be... 47 Telecommunication 5 2014-10-01 2014-10-01 false Automatically controlled digital station. 97...

47 CFR 97.221 - Automatically controlled digital station.

Code of Federal Regulations, 2011 CFR

2011-10-01

... station. (a) This rule section does not apply to an auxiliary station, a beacon station, a repeater station, an earth station, a space station, or a space telecommand station. (b) A station may be... 47 Telecommunication 5 2011-10-01 2011-10-01 false Automatically controlled digital station. 97...

Index of stations: surface-water data-collection network of Texas, September 1999

USGS Publications Warehouse

Gandara, Susan C.; Barbie, Dana L.

2001-01-01

As of September 30, 1999, the surface-water data-collection network of Texas (table 1) included 321 continuous-record streamflow stations (D), 20 continuous-record gage-height only stations (G), 24 crest-stage partial-record stations (C), 40 floodhydrograph partial-record stations (H), 25 low-flow partial-record stations (L), 1 continuous-record temperature station (M1), 25 continuous-record temperature and specific conductance stations (M2), 17 continuous-record temperature, specific conductance, dissolved oxygen, and pH stations (M4), 4 daily water-quality stations (Qd), 115 periodic water-quality stations (Qp), 17 reservoir/lake surveys for water quality stations (Qs), 85 continuous or daily reservoircontent stations (R), and 10 daily precipitation stations (Pd). Plate 1 identifies the major river basins in Texas and shows the location of the stations listed in table 1. Table 1 shows the station number and name, latitude and longitude, type of station, and office responsible for the collection of the data and maintenance of the record. An 8-digit permanent numerical designation for all gaging stations has been adopted on a nationwide basis; stations are numbered and listed in downstream order. In the downstream direction along the main stem, all stations on a tributary entering between two main-stem stations are listed between these two stations. A similar order is followed in listing stations by first rank, second rank, and other ranks of tributaries. The rank of any tributary, with respect to the stream to which it is an immediate tributary, is indicated by an indention in the table. Each indention represents one rank. This downstream order and system of indention shows which gaging stations are on tributaries between any two stations on a main stem and the rank of the tributary on which each gaging station is situated.

Operation of hydrologic data collection stations by the U.S. Geological Survey in 1987

USGS Publications Warehouse

Condes de la Torre, Alberto

1987-01-01

The U.S. Geological Survey operates hydrologic data collection stations nationwide which serve the needs of all levels of government, the private sector, and the general public, for water resources information. During fiscal year 1987, surface water discharge was determined at 10,624 stations; stage data on streams, reservoirs, and lakes were recorded at 1,806 stations; and various surface water quality characteristics were determined at 2,901 stations. In addition, groundwater levels were measured at 32,588 stations, and the quality of groundwater was determined at 9,120 stations. Data on sediment were collected daily at 174 stations and on a periodic basis at 878 stations. Information on precipitation quantity was collected at 909 stations, and the quality of precipitation was analyzed at 78 stations. Data collection platforms for satellite telemetry of hydrologic information were used at 2,292 Geological Survey stations. Funding for the hydrologic stations was derived, either solely or from a combination, from three major sources - the Geological Survey 's Federal Program appropriation, the Federal-State Cooperative Program, and reimbursements from other Federal agencies. The number of hydrologic stations operated by the Geological Survey declined from fiscal year 1983 to 1987. The number of surface water discharge stations were reduced by 452 stations; surface water quality stations declined by 925 stations; groundwater level stations declined by 1,051 stations; while groundwater quality stations increased by 1,472 stations. (Author 's abstract)

47 CFR 74.635 - Unattended operation.

Code of Federal Regulations, 2013 CFR

2013-10-01

... stations, and TV microwave booster stations may be operated unattended under the following conditions: (1... persons; (3) TV relay stations, TV STL stations, TV translator relay stations, and TV microwave booster..., an STL station (and any TV microwave booster station) associated with a TV broadcast station operated...

47 CFR 74.635 - Unattended operation.

Code of Federal Regulations, 2012 CFR

2012-10-01

... stations, and TV microwave booster stations may be operated unattended under the following conditions: (1... persons; (3) TV relay stations, TV STL stations, TV translator relay stations, and TV microwave booster..., an STL station (and any TV microwave booster station) associated with a TV broadcast station operated...

47 CFR 74.635 - Unattended operation.

Code of Federal Regulations, 2014 CFR

2014-10-01

... stations, and TV microwave booster stations may be operated unattended under the following conditions: (1... persons; (3) TV relay stations, TV STL stations, TV translator relay stations, and TV microwave booster..., an STL station (and any TV microwave booster station) associated with a TV broadcast station operated...

47 CFR 74.635 - Unattended operation.

Code of Federal Regulations, 2011 CFR

2011-10-01

... stations, and TV microwave booster stations may be operated unattended under the following conditions: (1... persons; (3) TV relay stations, TV STL stations, TV translator relay stations, and TV microwave booster..., an STL station (and any TV microwave booster station) associated with a TV broadcast station operated...

Index of stations: surface-water data-collection network of Texas, September 1998

USGS Publications Warehouse

Gandara, Susan C.; Barbie, Dana L.

1999-01-01

As of September 30, 1998, the surface-water data-collection network of Texas (table 1) included 313 continuous-recording streamflow stations (D), 22 gage-height record only stations (G), 23 crest-stage partial-record stations (C), 39 flood-hydrograph partial-record stations (H), 25 low-flow partial-record stations (L), 1 continuous-recording temperature station (M1), 25 continuous-recording temperature and conductivity stations (M2), 3 continuous-recording temperature, conductivity, and dissolved oxygen stations (M3), 13 continuous-recording temperature, conductivity, dissolved oxygen, and pH stations (M4), 5 daily chemical-quality stations (Qd), 133 periodic chemical-quality stations (Qp), 16 reservoir/lake surveys for water quality (Qs), and 70 continuous or daily reservoir-content stations (R). Plate 1 identifies the major river basins in Texas and shows the location of the stations listed in table 1.

47 CFR 80.71 - Operating controls for stations on land.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Operating controls for stations on land. 80.71... SERVICES STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Station Requirements-Land Stations § 80.71 Operating controls for stations on land. Each coast station, Alaska-public fixed station...

Yap4 PKA- and GSK3-dependent phosphorylation affects its stability but not its nuclear localization.

PubMed

Pereira, Jorge; Pimentel, Catarina; Amaral, Catarina; Menezes, Regina A; Rodrigues-Pousada, Claudina

2009-12-01

Yap4 is a nuclear-resident transcription factor induced in Saccharomyces cerevisiae when exposed to several stress conditions, which include mild hyperosmotic and oxidative stress, temperature shift or metal exposure. This protein is also phosphorylated. Here we report that this modification is driven by PKA and GSK3. In order to ascertain whether Yap4 is directly or indirectly phosphorylated by PKA, we searched for stress and PKA-related kinases that could phosphorylate Yap4. We show that phosphorylation is independent of the kinases Rim15, Yak1, Sch9, Slt2, Ste20 and Ptk2. In addition, we showed that Yap4 phosphorylation is also abrogated in the triple GSK3 mutant mck1 rim11 yol128c. Furthermore, our data reveal that Yap4 nuclear localization is independent of its phosphorylation state. This protein has several putative phosphorylation sites, but only the mutation of residues T192 and S196 impairs its phosphorylation under different stress conditions. The ability of the non-phosphorylated forms of Yap4 to partially rescue the hog1 severe sensitivity phenotype is not affected, suggesting that Yap4 activity is maintained in the absence of phosphorylation. However, this modification seems to be required for stability of the protein, as the non-phosphorylated form has a shorter half-life than the phosphorylated one.

Dominant lactic acid bacteria and their technological properties isolated from the Himalayan ethnic fermented milk products.

PubMed

Dewan, Sailendra; Tamang, Jyoti Prakash

2007-10-01

Ethnic people of the Himalayan regions of India, Nepal, Bhutan and China consume a variety of indigenous fermented milk products made from cows milk as well as yaks milk. These lesser-known ethnic fermented foods are dahi, mohi, chhurpi, somar, philu and shyow. The population of lactic acid bacteria (LAB) ranged from 10(7) to 10(8) cfu/g in these Himalayan milk products. A total of 128 isolates of LAB were isolated from 58 samples of ethnic fermented milk products collected from different places of India, Nepal and Bhutan. Based on phenotypic characterization including API sugar test, the dominant lactic acid bacteria were identified as Lactobacillus bifermentans, Lactobacillus paracasei subsp. pseudoplantarum, Lactobacillus kefir, Lactobacillus hilgardii, Lactobacillus alimentarius, Lactobacillus paracasei subsp. paracasei, Lactobacillus plantarum, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris and Enterococcus faecium. LAB produced a wide spectrum of enzymes and showed high galactosidase, leucine-arylamidase and phosphatase activities. They showed antagonistic properties against selected Gram-negative bacteria. None of the strains produced bacteriocin and biogenic amines under the test conditions used. Most strains of LAB coagulated skim milk with a moderate drop in pH. Some strains of LAB showed a high degree of hydrophobicity, suggesting these strains may have useful adhesive potential. This paper is the first report on functional lactic acid bacterial composition in some lesser-known ethnic fermented milk products of the Himalayas.

Aerosol transport over Siberia: analysis of the summer 2013 YAK-AEROSIB aircraft campaign

NASA Astrophysics Data System (ADS)

Ancellet, Gerard; Penner, Johannes; Kokhanenko, Grigorii; Arshinov, Mikhail; Chernov, Dimitry; Kozlov, Valery; Paris, Jean Daniel; Pruvost, Arnaud; Belan, Boris; Nedelec, Philippe; Pelon, Jacques; Law, Kathy

2014-05-01

Transport and transformation of aerosols related to forest fires and Eastern Asia anthropogenic emissions have been identified as very important questions to understand the Arctic climate. Two aircraft campaigns have been conducted over Siberia in summer 2012 and 2013 with in-situ measurements by aerosol spectrometers and also by a 532 nm backscatter lidar in 2013. The aerosol data can be also combined with CO measurements measured on-board the aircraft to identify the aerosol pollution sources. The analysis of the transport processes has been performed with the FLEXPART Lagrangian model run either in the forward or backward mode. While the 2012 campaign is characterized by anticyclonic conditions and strong forest fire emissions, the 2013 campaign corresponds to upward lifting of Northern China emissions. Comparisons with satellite data obtained with the CALIPSO mission for the two summer periods will be presented to identify the spatial extent and the temporal evolution of the pollution plumes and also to test the ability of the satellite data to derive the aerosol types. This work was funded by CNRS (France), the French Ministry of Foreign Affairs, CEA (France), Presidium of RAS (Program No. 4), Brunch of Geology, Geophysics and Mining Sciences of RAS (Program No. 5), Interdisciplinary integration projects of Siberian Branch of RAS (No. 35, No. 70, No. 131), Russian Foundation for Basic Research (grants No 14-05-00526, 14-05-00590).

The YAK-AEROSIB transcontinental aircraft campaigns: new insights on the transport of CO2, CO and O3 across Siberia

NASA Astrophysics Data System (ADS)

Paris, J.-D.; Ciais, P.; Nédélec, P.; Ramonet, M.; Belan, B. D.; Arshinov, M. Yu.; Golitsyn, G. S.; Granberg, I.; Stohl, A.; Cayez, G.; Athier, G.; Boumard, F.; Cousin, J.-M.

2008-09-01

Two airborne campaigns were carried out to measure the tropospheric concentrations and variability of CO2, CO and O3 over Siberia. In order to quantify the influence of remote and regional natural and anthropogenic sources, we analysed a total of 52 vertical profiles of these species collected in April and September 2006, every ~200 km and up to 7 km altitude. CO2 and CO concentrations were high in April 2006 (respectively 385-390 ppm CO2 and 160-200 ppb CO) compared to background values. CO concentrations up to 220 ppb were recorded above 3.5 km over eastern Siberia, with enhancements in 500-1000 m thick layers. The presence of CO enriched air masses resulted from a quick frontal uplift of a polluted air mass exposed to northern China anthropogenic emissions and to fire emissions in northern Mongolia. A dominant Asian origin for CO above 4 km (71.0%) contrasted with a dominant European origin below this altitude (70.9%) was deduced both from a transport model analysis, and from the contrasted ΔCO/ΔCO2 ratio vertical distribution. In September 2006, a significant O3 depletion (~ -30 ppb) was repeatedly observed in the boundary layer, as diagnosed from virtual potential temperature profiles and CO2 gradients, compared to the free troposphere aloft, suggestive of a strong O3 deposition over Siberian forests.

Preliminary results of an aircraft system based on near-IR diode lasers for continuous measurements of the concentration of methane, carbon dioxide, water and its isotopes

NASA Astrophysics Data System (ADS)

Nadezhdinsky, A. I.; Ponurovsky, Ya. Ya.; Shapovalov, Y. P.; Popov, I. P.; Stavrovsky, D. B.; Khattatov, V. U.; Galaktionov, V. V.; Kuzmichev, A. S.

2012-11-01

The Federal Agency for Hydrometeorology of the Russian Federation created the flying laboratory on board the passenger airplane Yak-42D for geophysical monitoring of the environment, including aircraft measurements of vertical concentrations of greenhouse gases in the troposphere. Within the limits of this project, General Physics Institute of the Russian Academy of Science developed airborne tunable diode laser spectrometer (TDLS) on the basis of diode lasers of a near-IR range for measurement of the altitude profiles of CO2, CH4, H2O and its isotopes. TDLS complex was integrated aboard in standard 19-in. rack. Air samples, taken over an aircraft on the pipeline, were injected into the optical cell. Using the system of inflow and heating, the air was set laminar with a flowrate of 0.2 l/s at a reduced pressure of 100 mbar for detecting narrow absorption lines of water vapor isotopes. For registration of the absorption spectra and for the measurement of greenhouse gas concentrations in online mode, modulation-correlation technique was used. Diode laser spectrometer output data were transferred to the airborne central computer. Sensitivity of TDLS measurements was 20-30 ppm for water, 3-4 ppm for CO2 and 20-25 ppb for CH4. Time of one-unit measurement is about 30 ms.

From 'third pole' to north pole: a Himalayan origin for the arctic fox.

PubMed

Wang, Xiaoming; Tseng, Zhijie Jack; Li, Qiang; Takeuchi, Gary T; Xie, Guangpu

2014-07-22

The 'third pole' of the world is a fitting metaphor for the Himalayan-Tibetan Plateau, in allusion to its vast frozen terrain, rivalling the Arctic and Antarctic, at high altitude but low latitude. Living Tibetan and arctic mammals share adaptations to freezing temperatures such as long and thick winter fur in arctic muskox and Tibetan yak, and for carnivorans, a more predatory niche. Here, we report, to our knowledge, the first evolutionary link between an Early Pliocene (3.60-5.08 Myr ago) fox, Vulpes qiuzhudingi new species, from the Himalaya (Zanda Basin) and Kunlun Mountain (Kunlun Pass Basin) and the modern arctic fox Vulpes lagopus in the polar region. A highly hypercarnivorous dentition of the new fox bears a striking resemblance to that of V. lagopus and substantially predates the previous oldest records of the arctic fox by 3-4 Myr. The low latitude, high-altitude Tibetan Plateau is separated from the nearest modern arctic fox geographical range by at least 2000 km. The apparent connection between an ancestral high-elevation species and its modern polar descendant is consistent with our 'Out-of-Tibet' hypothesis postulating that high-altitude Tibet was a training ground for cold-environment adaptations well before the start of the Ice Age. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Travels with the Fossil Hunters

NASA Astrophysics Data System (ADS)

Whybrow, Peter J.

2000-04-01

Whether dodging bullets in West Africa, or rabid dogs in Pakistan, surviving yak-butter tea in Tibet, or eating raw fish in China, the life of a globe-trotting fossil hunter is often hazardous and always filled with surprises. Travels with the Fossil Hunters lets readers share the wonder, joys of discovery, and excitement of these intrepid scientists. Packed with more than 100 beautiful, full-color photographs, the volume takes readers on twelve expeditions to remote parts of the world in search of diverse fossil remains, from those of dinosaurs to human ancestors. Each expedition by paleontologists from London's Natural History Museum reveals the problems and challenges of working in extreme conditions, from the deserts of the Sahara and Yemen to the frozen wastes of Antarctica, from the mountains of India to the forests of Latvia. Along the way they also describe the paleontology and geology of the countries they visit and the scientific reasons for their expeditions. With a foreword from Sir David Attenborough and an introduction from Richard Fortey, this fascinating book will appeal to amateur and professional fossil hunters alike and to readers interested in accounts of exotic locales. Peter Whybrow is a research scientist at the Natural History Museum, London. His research interests include Arabian Miocene vertebrates, paleoclimates, paleogeography, and biotic diversity. He is senior editor with A. Hill of Fossil Vertebrates of Arabia (Yale University Press, New Haven, 1999).

Algal dual-specificity tyrosine phosphorylation-regulated kinase, triacylglycerol accumulation regulator1, regulates accumulation of triacylglycerol in nitrogen or sulfur deficiency.

PubMed

Kajikawa, Masataka; Sawaragi, Yuri; Shinkawa, Haruka; Yamano, Takashi; Ando, Akira; Kato, Misako; Hirono, Masafumi; Sato, Naoki; Fukuzawa, Hideya

2015-06-01

Although microalgae accumulate triacylglycerol (TAG) and starch in response to nutrient-deficient conditions, the regulatory mechanisms are poorly understood. We report here the identification and characterization of a kinase, triacylglycerol accumulation regulator1 (TAR1), that is a member of the yeast (Saccharomyces cerevisiae) Yet another kinase1 (Yak1) subfamily in the dual-specificity tyrosine phosphorylation-regulated kinase family in a green alga (Chlamydomonas reinhardtii). The kinase domain of TAR1 showed auto- and transphosphorylation activities. A TAR1-defective mutant, tar1-1, accumulated TAG to levels 0.5- and 0.1-fold of those of a wild-type strain in sulfur (S)- and nitrogen (N)-deficient conditions, respectively. In N-deficient conditions, tar1-1 showed more pronounced arrest of cell division than the wild type, had increased cell size and cell dry weight, and maintained chlorophyll and photosynthetic activity, which were not observed in S-deficient conditions. In N-deficient conditions, global changes in expression levels of N deficiency-responsive genes in N assimilation and tetrapyrrole metabolism were noted between tar1-1 and wild-type cells. These results indicated that TAR1 is a regulator of TAG accumulation in S- and N-deficient conditions, and it functions in cell growth and repression of photosynthesis in conditions of N deficiency. © 2015 American Society of Plant Biologists. All Rights Reserved.

System for a displaying at a remote station data generated at a central station and for powering the remote station from the central station

NASA Technical Reports Server (NTRS)

Perry, J. C. (Inventor)

1980-01-01

A system for displaying at a remote station data generated at a central station and for powering the remote station from the central station is presented. A power signal is generated at the central station and time multiplexed with the data and then transmitted to the remote station. An energy storage device at the remote station is responsive to the transmitted power signal to provide energizing power for the circuits at the remote station during the time interval data is being transmitted to the remote station. Energizing power for the circuits at the remote station is provided by the power signal itself during the time this signal is transmitted. Preferably the energy storage device is a capacitor which is charged by the power signal during the time the power is transmitted and is slightly discharged during the time the data is transmitted to energize the circuits at the remote station.

[Determination of the numbers of monitoring medical institutions necessary for estimating incidence rates in the surveillance of infectious diseases in Japan].

PubMed

Hashimoto, S; Murakami, Y; Taniguchi, K; Nagai, M

1999-12-01

Our purpose was to determine the number of monitoring stations (medical institutions) necessary for estimating incidence rates in the surveillance system of infectious diseases in Japan. Infectious diseases were selected by the type of monitoring stations: 15 diseases in pediatrics stations, influenza in influenza stations, 3 diseases in ophthalmology stations and 5 diseases in the stations of sexually transmitted diseases (STD). For each type of monitoring station, 5 cases of the number of monitoring stations in each health center, including the number determined from presently established standards and the actual number in 1997, were given. It was assumed that monitoring stations were randomly selected among medical institutions in health centers. For each infectious disease, each case and each type of monitoring station, standard error rates of estimated numbers of incidence cases in the whole country were calculated in 1993-1997 using the data of the surveillance of infectious diseases. Among 5 cases of monitoring stations, the case satisfied the condition that those standard error rates were lower than the critical values, was selected. The critical values were 5% in pediatrics and influenza stations, and 10% in ophthalmology and STD stations. The numbers of monitoring stations in the selected cases were 3,000 in pediatrics stations, 5,000 in influenza stations (including all pediatrics stations), 605 in ophthalmology stations and 900 in STD stations.

47 CFR 80.1119 - Receipt and acknowledgement of distress alerts by coast stations and coast earth stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... by coast stations and coast earth stations. 80.1119 Section 80.1119 Telecommunication FEDERAL... § 80.1119 Receipt and acknowledgement of distress alerts by coast stations and coast earth stations. (a... for coast stations.) (b) Coast earth stations in receipt of distress alerts must ensure that they are...

47 CFR 80.1119 - Receipt and acknowledgement of distress alerts by coast stations and coast earth stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... by coast stations and coast earth stations. 80.1119 Section 80.1119 Telecommunication FEDERAL... § 80.1119 Receipt and acknowledgement of distress alerts by coast stations and coast earth stations. (a... for coast stations.) (b) Coast earth stations in receipt of distress alerts must ensure that they are...

47 CFR 80.1119 - Receipt and acknowledgement of distress alerts by coast stations and coast earth stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... by coast stations and coast earth stations. 80.1119 Section 80.1119 Telecommunication FEDERAL... § 80.1119 Receipt and acknowledgement of distress alerts by coast stations and coast earth stations. (a... for coast stations.) (b) Coast earth stations in receipt of distress alerts must ensure that they are...

47 CFR 80.1119 - Receipt and acknowledgement of distress alerts by coast stations and coast earth stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... by coast stations and coast earth stations. 80.1119 Section 80.1119 Telecommunication FEDERAL... § 80.1119 Receipt and acknowledgement of distress alerts by coast stations and coast earth stations. (a... for coast stations.) (b) Coast earth stations in receipt of distress alerts must ensure that they are...

47 CFR 80.1119 - Receipt and acknowledgement of distress alerts by coast stations and coast earth stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... by coast stations and coast earth stations. 80.1119 Section 80.1119 Telecommunication FEDERAL... § 80.1119 Receipt and acknowledgement of distress alerts by coast stations and coast earth stations. (a... for coast stations.) (b) Coast earth stations in receipt of distress alerts must ensure that they are...

Index of surface-water stations in Texas, January 1984

USGS Publications Warehouse

Carrillo, E.R.; Buckner, H.D.

1984-01-01

This index shows the station number and name, latitude and longitude, type of data collected, and the office principally responsible for the data collection (table 1). An 8-digit permanent numerical designation for gaging stations has been adopted on a nationwide basis; stations are numbered and listed in downstream order. In the downstream direction along the main stem, all stations on a tributary entering above a main-stem station are listed before that station. A tributary entering between two main-stem stations is listed between them. A similar order is followed in listing stations on first rank, second rank, and other ranks of tributaries. To indicate the rank of any tributary on which a gaging station is situated and the stream to which it is an immediate tributary, each indention in the listing of gaging stations represent one rank. This downstream order and system of indention show which gaging stations are on tributaries between any two stations on a main stem and the rank of the tributary on which each gaging station is situated. On plates 1 and 2 the 8-digit station number is abbreviated because of space limitation.

Index of surface-water stations in Texas, January 1985

USGS Publications Warehouse

Carrillo, E.R.; Buckner, H.D.; Rawson, Jack

1984-01-01

This index shows the station number -and name, latitude and longitude, type of data collected, and the office principally responsible for the data collection (table 1). An 8-digit permanent numerical designation for gaging stations has been adopted on a nationwide basis; stations are numbered and listed in downstream order. In the downstream direction along the main stem, all stations on a tributary entering above a main-stem station are listed before that station. A tributary entering between two main-stem stations is listed between them. A similar order is followed in listing stations on first rank, second rank, and other ranks of tributaries. To indicate the rank of any tributary on which a gaging station is situated and the stream to which it is an immediate tributary, each indention in the listing of gaging stations represent one rank. This downstream order and system of indention show which gaging stations are on tributaries between any two stations on a main stem and the rank of the tributary on which each gaging station is situated. On plates 1 and 2, the 8-digit station number is abbreviated because of space limitation.

Index of surface-water stations in Texas, January 1987

USGS Publications Warehouse

Rawson, Jack; Carrillo, E.R.; Buckner, H.D.

1987-01-01

This index shows the station number and name, latitude and longitude, type of data collected, and the office principally responsible for the data collection (table 1). An 8-digit permanent numerical designation for gaging stations has been adopted on a nationwide basis; stations are numbered and listed in downstream order. In the downstream direction along the main stem, all stations on a tributary entering above a main-stem station are listed before that station. A tributary entering between two main-stem stations is listed between them. A similar order is followed in listing stations on first rank, second rank, and other ranks of tributaries. To indicate the rank of any tributary on which a gaging station is situated and the stream to which it is an immediate tributary, each indention in the listing of gaging stations represent one rank. This downstream order and system of indention show which gaging stations are on tributaries between any two stations on a main stem and the rank of the tributary on which each gaging station is situated. On plates 1 and 2, the 8-digit station number is abbreviated because of space limitation.

Index of surface-water stations in Texas, January 1988

USGS Publications Warehouse

Rawson, Jack; Carrillo, E.R.; Buckner, H.D.

1988-01-01

This index shows the station number and name, latitude and longitude, type of data collected, and the office principally responsible for the data collection (table 1). An 8-digit permanent numerical designation for gaging stations has been adopted on a nationwide basis; stations are numbered and listed in downstream order. In the downstream direction along the main stem, all stations on a tributary entering above a main-stem station are listed before that station. A tributary entering between two main-stem stations is listed between them. A similar order is followed in listing stations on first rank, second rank, and other ranks of tributaries. To indicate the rank of any tributary on which a gaging station is situated and the stream to which it is an immediate tributary, each indention in the listing of gaging stations represent one rank. This downstream order and system of indention show which gaging stations are on tributaries between any two stations on a main stem and the rank of the tributary on which each gaging station is situated. On plates 1 and 2, the 8-digit station number is abbreviated because of space limitation.

47 CFR 74.635 - Unattended operation.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Stations § 74.635 Unattended operation. (a) TV relay stations, TV translator relay stations, TV STL... persons; (3) TV relay stations, TV STL stations, TV translator relay stations, and TV microwave booster... control point. Additionally, a TV translator relay station (and any associated TV microwave booster...

47 CFR 95.29 - Channels available.

Code of Federal Regulations, 2010 CFR

2010-10-01

... SERVICES General Mobile Radio Service (GMRS) § 95.29 Channels available. (a) For a base station, fixed station, mobile station, or repeater station (a GMRS station that simultaneously retransmits the... non-individual, a mobile station or a small base station operating in the simplex mode may transmit on...

47 CFR 90.305 - Location of stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... stations. (a) The transmitter site(s) for base station(s), including mobile relay stations, shall be.... (b) Mobile units shall be operated within 48 km. (30 mi.) of their associated base station or...). (c) Control stations must be located within the area of operation of the mobile units. (d) Base and...

Space Station Freedom Utilization Conference

NASA Technical Reports Server (NTRS)

1992-01-01

The topics addressed in Space Station Freedom Utilization Conference are: (1) space station freedom overview and research capabilities; (2) space station freedom research plans and opportunities; (3) life sciences research on space station freedom; (4) technology research on space station freedom; (5) microgravity research and biotechnology on space station freedom; and (6) closing plenary.

47 CFR 74.601 - Classes of TV broadcast auxiliary stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... or other purposes as authorized in § 74.631. (d) TV translator relay station. A fixed station used... low power TV stations, unless specifically otherwise indicated. (f) TV microwave booster station. A... Television Broadcast Auxiliary Stations § 74.601 Classes of TV broadcast auxiliary stations. (a) TV pickup...

47 CFR 74.601 - Classes of TV broadcast auxiliary stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... or other purposes as authorized in § 74.631. (d) TV translator relay station. A fixed station used... low power TV stations, unless specifically otherwise indicated. (f) TV microwave booster station. A... Television Broadcast Auxiliary Stations § 74.601 Classes of TV broadcast auxiliary stations. (a) TV pickup...

47 CFR 74.601 - Classes of TV broadcast auxiliary stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... or other purposes as authorized in § 74.631. (d) TV translator relay station. A fixed station used... low power TV stations, unless specifically otherwise indicated. (f) TV microwave booster station. A... Television Broadcast Auxiliary Stations § 74.601 Classes of TV broadcast auxiliary stations. (a) TV pickup...

47 CFR 74.601 - Classes of TV broadcast auxiliary stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... or other purposes as authorized in § 74.631. (d) TV translator relay station. A fixed station used... low power TV stations, unless specifically otherwise indicated. (f) TV microwave booster station. A... Television Broadcast Auxiliary Stations § 74.601 Classes of TV broadcast auxiliary stations. (a) TV pickup...

47 CFR 74.783 - Station identification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Booster Stations § 74.783 Station identification. (a) Each low power TV and TV translator station not... suffix “-LP.” (f) TV broadcast booster station shall be identified by their primary stations by...

47 CFR 74.783 - Station identification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Booster Stations § 74.783 Station identification. (a) Each low power TV and TV translator station not... suffix “-LP.” (f) TV broadcast booster station shall be identified by their primary stations by...

47 CFR 74.783 - Station identification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Booster Stations § 74.783 Station identification. (a) Each low power TV and TV translator station not... suffix “-LP.” (f) TV broadcast booster station shall be identified by their primary stations by...

Data transmission system with distributed microprocessors

DOEpatents

Nambu, Shigeo

1985-01-01

A data transmission system having a common request line and a special request line in addition to a transmission line. The special request line has priority over the common request line. A plurality of node stations are multi-drop connected to the transmission line. Among the node stations, a supervising station is connected to the special request line and takes precedence over other slave stations to become a master station. The master station collects data from the slave stations. The station connected to the common request line can assign a master control function to any station requesting to be assigned the master control function within a short period of time. Each station has an auto response control circuit. The master station automatically collects data by the auto response controlling circuit independently of the microprocessors of the slave stations.

H2FIRST Reference Station Design Task: Project Deliverable 2-2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pratt, Joseph; Terlip, Danny; Ainscough, Chris

2015-04-20

This report presents near-term station cost results and discusses cost trends of different station types. It compares various vehicle rollout scenarios and projects realistic near-term station utilization values using the station infrastructure rollout in California as an example. It describes near-term market demands and matches those to cost-effective station concepts. Finally, the report contains detailed designs for five selected stations, which include piping and instrumentation diagrams, bills of materials, and several site-specific layout studies that incorporate the setbacks required by NFPA 2, the National Fire Protection Association Hydrogen Technologies Code. This work identified those setbacks as a significant factor affectingmore » the ability to site a hydrogen station, particularly liquid stations at existing gasoline stations. For all station types, utilization has a large influence on the financial viability of the station.« less

47 CFR 80.503 - Cooperative use of facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... station or marine utility station on shore may install ship radio stations on board United States... verifying that the ship station licensee has the sole right of control of the ship stations, that the vessel operators must use the ship stations subject to the orders and instructions of the coast station or marine...

47 CFR 73.6016 - Digital Class A TV station protection of TV broadcast stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 4 2011-10-01 2011-10-01 false Digital Class A TV station protection of TV...) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES Class A Television Broadcast Stations § 73.6016 Digital Class A TV station protection of TV broadcast stations. Digital Class A TV stations must protect...

47 CFR 73.6016 - Digital Class A TV station protection of TV broadcast stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 4 2010-10-01 2010-10-01 false Digital Class A TV station protection of TV...) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES Class A Television Broadcast Stations § 73.6016 Digital Class A TV station protection of TV broadcast stations. Digital Class A TV stations must protect...

47 CFR 74.1283 - Station identification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... FM Broadcast Booster Stations § 74.1283 Station identification. (a) The call sign of an FM broadcast... of an FM booster station will consist of the call sign of the primary station followed by the letters “FM” and the number of the booster station being authorized, e.g., WFCCFM-1. (c) A translator station...

47 CFR 74.1283 - Station identification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... FM Broadcast Booster Stations § 74.1283 Station identification. (a) The call sign of an FM broadcast... of an FM booster station will consist of the call sign of the primary station followed by the letters “FM” and the number of the booster station being authorized, e.g., WFCCFM-1. (c) A translator station...

47 CFR 74.1283 - Station identification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... FM Broadcast Booster Stations § 74.1283 Station identification. (a) The call sign of an FM broadcast... of an FM booster station will consist of the call sign of the primary station followed by the letters “FM” and the number of the booster station being authorized, e.g., WFCCFM-1. (c) A translator station...

47 CFR 74.1283 - Station identification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... FM Broadcast Booster Stations § 74.1283 Station identification. (a) The call sign of an FM broadcast... of an FM booster station will consist of the call sign of the primary station followed by the letters “FM” and the number of the booster station being authorized, e.g., WFCCFM-1. (c) A translator station...

78 FR 53757 - Information Collections Being Submitted for Review and Approval to the Office of Management and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-30

...'s channel number, as stated on the station's license, and/or the station's network affiliation may... Stations, choosing to include the station's channel number in the station identification must use the station's major channel number and may distinguish multicast program streams. For example, a DTV station...

47 CFR 25.172 - Requirements for reporting space station control arrangements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... case of a non-U.S.-licensed space station, prior to commencing operation with U.S. earth stations. (1... earth station(s) communicating with the space station from any site in the United States. (3) The location, by city and country, of any telemetry, tracking, and command earth station that communicates with...

47 CFR 74.709 - Land mobile station protection.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Translator, and TV Booster Stations § 74.709 Land mobile station protection. (a) Stations in the Land Mobile... caused by low power TV or TV translator stations, and low power TV and TV translator stations must accept... translator station application will not be accepted if it specifies a site that is within the protected...

International VLBI Service for Geodesy and Astrometry 2004 Annual Report

NASA Technical Reports Server (NTRS)

Behrend, Dirk (Editor); Baver, Karen D. (Editor)

2005-01-01

Contents include the following: Combination Studies using the Cont02 Campaign. Coordinating Center report. Analysis coordinator report. Network coordinator report. IVS Technology coordinator report. Algonquin Radio observatory. Fortaleza Station report for 2004. Gilmore Creek Geophysical Observatory. Goddard Geophysical and Astronomical observatory. Hartebeesthoek Radio Astronomy Observatory (HartRAO). Hbart, Mt Pleasant, station report for 2004. Kashima 34m Radio Telescope. Kashima and Koganei 11-m VLBI Stations. Kokee Park Geophysical Observatory. Matera GGS VLBI Station. The Medicina Station status report. Report of the Mizusawa 10m Telescope. Noto Station Activity. NYAL Ny-Alesund 20 metre Antenna. German Antarctic receiving Station (GARS) O'higgins. The IVS network station Onsala space Observatory. Sheshan VLBI Station report for 2004. 10 Years of Geodetic Experiments at the Simeiz VLBI Station. Svetloe RAdio Astronomical Observatory. JARE Syowa Station 11-m Antenna, Antarctica. Geodetic Observatory TIGO in Concepcion. Tsukuba 32-m VLBI Station. Nanshan VLBI Station Report. Westford Antenna. Fundamental-station Wettzell 20m Radiotelescope. Observatorio Astroonomico Nacional Yebes. Yellowknife Observatory. The Bonn Geodetic VLBI Operation Center. CORE Operation Center Report. U.S. Naval Observatory Operation Center. The Bonn Astro/Geo Mark IV Correlator.

jsc2018m000321_Destination_Station-MP4

NASA Image and Video Library

2018-05-11

Destination Station---- When you can’t come to the International Space Station, the essence of the space station can come to you! Beginning May 15, Destination Station arrives in Salt Lake City, UT to share the impacts of the station on our daily lives. Here’s a peek at some of the ways you can learn more about what the International Space Station is doing right now. ___________________________ FOLLOW THE SPACE STATION! Twitter: https://twitter.com/Space_Station Facebook: https://www.facebook.com/ISS Instagram: https://instagram.com/iss/

Principal facts for 408 gravity stations in the vicinity of the Talkeetna Mountains, south-central Alaska

USGS Publications Warehouse

Morin, Robert L.; Glen, Jonathan M.G.

2003-01-01

Gravity data were collected between 1999 and 2002 along transects in the Talkeetna Mountains of south-central Alaska as part of a geological and geophysical study of the framework geology of the region. The study area lies between 61° 30’ and 63° 45’ N. latitude and 145° and 151° W. longitude. This data set includes 408 gravity stations. These data, combined with the pre-existing 3,286 stations, brings the total data in this area to 3,694 gravity stations. Principal facts for the 408 new gravity stations and the 15 gravity base stations used for control are listed in this report. During the summer of 1999, a gravity survey was conducted in the western Talkeetna Mountains. Measurements at 55 gravity stations were made. One gravity base station was used for control for this survey. This base station, STEP, is located at the Stephan Lake Lodge on Stephan Lake. The observed gravity of this station was calculated based on an indirect tie to base station ANCL in Anchorage. The temporary base used to tie between STEP and ANCL was REGL in Anchorage. During the summer of 2000, a gravity survey was conducted in the western Talkeetna Mountains. Measurements at 56 gravity stations were made. One gravity base station was used for control for this survey. This base station, GRHS, is located at the Gracious House Lodge on the Denali Highway. The observed gravity of this station was calculated based on multiple ties to base stations D87, and D57 along the Denali Highway. During the summer of 2001, a gravity survey was conducted in the western Talkeetna Mountains. Measurements at 90 gravity stations were made. One gravity base station was used for control for this survey. This base station, HLML, is located at the High Lake Lodge. The observed gravity of this station was calculated based on multiple ties to base stations ANCU in Anchorage, PALH in Palmer, WASA in Wasilla, and TLKM in Talkeetna. Also during the summer of 2001, a gravity survey was conducted in the vicinity of Tangle Lakes. Measurements at 86 gravity stations were made. The Tangle Lakes area is located about 25 km west of Paxson and north of the Denali Highway. One gravity base station was used for control for this survey. This base station, TLIN, is located at the Tangle Lakes Inn. The observed gravity of this station was calculated based on multiple ties to base stations ANCU in Anchorage, PALH in Palmer, BD27 in Gulkana, B-07 on the Richardson Highway, and base stations D42, and D57 along the Denali Highway. During the summer of 2002, measurements at an additional 107 gravity stations were made in the vicinity of Tangle Lakes. Base station TLIN at the Tangle Lakes Inn was again used for control. Additional ties to base stations ANCU and B-07 were made.

Photovoltaic central station step and touch potential considerations in grounding system design

NASA Technical Reports Server (NTRS)

Engmann, G.

1983-01-01

The probability of hazardous step and touch potentials is an important consideration in central station grounding system design. Steam turbine generating station grounding system design is based on accepted industry practices and there is extensive in-service experience with these grounding systems. A photovoltaic (PV) central station is a relatively new concept and there is limited experience with PV station grounding systems. The operation and physical configuration of a PV central station is very different from a steam electric station. A PV station bears some similarity to a substation and the PV station step and touch potentials might be addressed as they are in substation design. However, the PV central station is a generating station and it is appropriate to examine the effect that the differences and similarities of the two types of generating stations have on step and touch potential considerations.

47 CFR 73.6018 - Digital Class A TV station protection of DTV stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 4 2011-10-01 2011-10-01 false Digital Class A TV station protection of DTV... RADIO SERVICES RADIO BROADCAST SERVICES Class A Television Broadcast Stations § 73.6018 Digital Class A TV station protection of DTV stations. Digital Class A TV stations must protect the DTV service that...

47 CFR 73.6018 - Digital Class A TV station protection of DTV stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 4 2010-10-01 2010-10-01 false Digital Class A TV station protection of DTV... RADIO SERVICES RADIO BROADCAST SERVICES Class A Television Broadcast Stations § 73.6018 Digital Class A TV station protection of DTV stations. Digital Class A TV stations must protect the DTV service that...

47 CFR 74.1204 - Protection of FM broadcast, FM Translator and LP100 stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... SERVICES FM Broadcast Translator Stations and FM Broadcast Booster Stations § 74.1204 Protection of FM... interference to the reception of such signal. (g) An application for an FM translator or an FM booster station... A stations and booster stations will be treated the same as their FM radio broadcast station...

47 CFR 74.1204 - Protection of FM broadcast, FM Translator and LP100 stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... SERVICES FM Broadcast Translator Stations and FM Broadcast Booster Stations § 74.1204 Protection of FM... interference to the reception of such signal. (g) An application for an FM translator or an FM booster station... A stations and booster stations will be treated the same as their FM radio broadcast station...

47 CFR 74.1204 - Protection of FM broadcast, FM Translator and LP100 stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... SERVICES FM Broadcast Translator Stations and FM Broadcast Booster Stations § 74.1204 Protection of FM... interference to the reception of such signal. (g) An application for an FM translator or an FM booster station... A stations and booster stations will be treated the same as their FM radio broadcast station...

47 CFR 74.1204 - Protection of FM broadcast, FM Translator and LP100 stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... SERVICES FM Broadcast Translator Stations and FM Broadcast Booster Stations § 74.1204 Protection of FM... interference to the reception of such signal. (g) An application for an FM translator or an FM booster station... A stations and booster stations will be treated the same as their FM radio broadcast station...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

Space Station Human Factors Research Review. Volume 3: Space Station Habitability and Function: Architectural Research

NASA Technical Reports Server (NTRS)

Cohen, Marc M. (Editor); Eichold, Alice (Editor); Heers, Susan (Editor)

1987-01-01

Articles are presented on a space station architectural elements model study, space station group activities habitability module study, full-scale architectural simulation techniques for space stations, and social factors in space station interiors.

47 CFR 80.1185 - Supplemental eligibility for mobile-satellite stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... station license for a ship earth station may be issued to: (1) The owner or operator of a ship. (2) A... operator of the ship aboard which the ship earth station is to be installed and operated. (b) A station license for a portable ship earth station may be issued to the owner or operator of portable earth station...

47 CFR 80.1185 - Supplemental eligibility for mobile-satellite stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... station license for a ship earth station may be issued to: (1) The owner or operator of a ship. (2) A... operator of the ship aboard which the ship earth station is to be installed and operated. (b) A station license for a portable ship earth station may be issued to the owner or operator of portable earth station...

47 CFR 80.1185 - Supplemental eligibility for mobile-satellite stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... station license for a ship earth station may be issued to: (1) The owner or operator of a ship. (2) A... operator of the ship aboard which the ship earth station is to be installed and operated. (b) A station license for a portable ship earth station may be issued to the owner or operator of portable earth station...

47 CFR 80.1185 - Supplemental eligibility for mobile-satellite stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... station license for a ship earth station may be issued to: (1) The owner or operator of a ship. (2) A... operator of the ship aboard which the ship earth station is to be installed and operated. (b) A station license for a portable ship earth station may be issued to the owner or operator of portable earth station...

47 CFR 80.1185 - Supplemental eligibility for mobile-satellite stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... station license for a ship earth station may be issued to: (1) The owner or operator of a ship. (2) A... operator of the ship aboard which the ship earth station is to be installed and operated. (b) A station license for a portable ship earth station may be issued to the owner or operator of portable earth station...

47 CFR 87.261 - Scope of service.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Aeronautical Enroute Stations, Aeronautical Fixed Stations, and Aircraft Data Link Land Test Stations Aeronautical Enroute Stations § 87.261 Scope of service. (a) Aeronautical enroute stations provide operational.... (b) Service must be provided to any aircraft station licensee who makes cooperative arrangements for...

47 CFR 74.682 - Station identification.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 4 2010-10-01 2010-10-01 false Station identification. 74.682 Section 74.682... Stations § 74.682 Station identification. (a) Each television broadcast auxiliary station operating with a transmitter output power of 1 watt or more must, when actually transmitting programs, transmit station...

47 CFR 73.1020 - Station license period.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., 2013. (4) Alabama and Georgia: (i) Radio stations, April 1, 2012. (ii) Television stations, April 1, 2013. (5) Arkansas, Louisiana and Mississippi: (i) Radio stations, June 1, 2012. (ii) Television stations, June 1, 2013. (6) Tennessee, Kentucky and Indiana: (i) Radio stations, August 1, 2012. (ii...

47 CFR 80.109 - Transmission to a plurality of mobile stations by a public coast station.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Transmission to a plurality of mobile stations... Procedures Operating Procedures-Land Stations § 80.109 Transmission to a plurality of mobile stations by a... mobile stations. ...

47 CFR 90.476 - Interconnection of fixed stations and certain mobile stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... mobile stations. 90.476 Section 90.476 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES PRIVATE LAND MOBILE RADIO SERVICES Transmitter Control Interconnected Systems § 90.476 Interconnection of fixed stations and certain mobile stations. (a) Fixed stations and...

47 CFR 95.139 - Adding a small base station or a small control station.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Adding a small base station or a small control... base station or a small control station. (a) Except for a GMRS system licensed to a non-individual, one or more small base stations or a small control station may be added to a GMRS system at any point...

Single transmission line interrogated multiple channel data acquisition system

DOEpatents

Fasching, George E.; Keech, Jr., Thomas W.

1980-01-01

A single transmission line interrogated multiple channel data acquisition system is provided in which a plurality of remote station/sensor circuits each monitors a specific process variable and each transmits measurement values over a single transmission line to a master interrogating station when addressed by said master interrogating station. Typically, as many as 330 remote stations may be parallel connected to the transmission line which may exceed 7,000 feet. The interrogation rate is typically 330 stations/second. The master interrogating station samples each station according to a shared, charging transmit-receive cycle. All remote station address signals, all data signals from the remote stations/sensors and all power for all of the remote station/sensors are transmitted via a single continuous terminated coaxial cable. A means is provided for periodically and remotely calibrating all remote sensors for zero and span. A provision is available to remotely disconnect any selected sensor station from the main transmission line.

47 CFR 90.1333 - Restrictions on the operation of mobile and portable stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... transmitted by a base station. (b) Any mobile/portable stations may communicate with any other mobile/portable... by a base station. (c) Airborne operations by mobile/portable stations is prohibited. ... portable stations. 90.1333 Section 90.1333 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED...

47 CFR 74.1201 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... FM Broadcast Booster Stations § 74.1201 Definitions. (a) FM translator. A station in the broadcasting... retransmitted by an FM broadcast translator station or an FM broadcast booster station. (e) AM or FM radio... indicates otherwise. (f) FM broadcast booster station. A station in the broadcasting service operated for...

47 CFR 74.1201 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... FM Broadcast Booster Stations § 74.1201 Definitions. (a) FM translator. A station in the broadcasting... retransmitted by an FM broadcast translator station or an FM broadcast booster station. (e) AM or FM radio... indicates otherwise. (f) FM broadcast booster station. A station in the broadcasting service operated for...

47 CFR 74.1201 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... FM Broadcast Booster Stations § 74.1201 Definitions. (a) FM translator. A station in the broadcasting... retransmitted by an FM broadcast translator station or an FM broadcast booster station. (e) AM or FM radio... indicates otherwise. (f) FM broadcast booster station. A station in the broadcasting service operated for...

47 CFR 74.1201 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... FM Broadcast Booster Stations § 74.1201 Definitions. (a) FM translator. A station in the broadcasting... retransmitted by an FM broadcast translator station or an FM broadcast booster station. (e) AM or FM radio... indicates otherwise. (f) FM broadcast booster station. A station in the broadcasting service operated for...

47 CFR 80.121 - Public coast stations using telegraphy.

Code of Federal Regulations, 2014 CFR

2014-10-01

... use the ship station selective calling number (5 digits) and its assigned coast station identification number (4 digits). Calls to ship stations must employ the following format: Ship station selective call number, repeated twice; “DE”, sent once; and coast station identification number, repeated twice. When...

47 CFR 80.121 - Public coast stations using telegraphy.

Code of Federal Regulations, 2012 CFR

2012-10-01

... use the ship station selective calling number (5 digits) and its assigned coast station identification number (4 digits). Calls to ship stations must employ the following format: Ship station selective call number, repeated twice; “DE”, sent once; and coast station identification number, repeated twice. When...

47 CFR 80.121 - Public coast stations using telegraphy.

Code of Federal Regulations, 2013 CFR

2013-10-01

... use the ship station selective calling number (5 digits) and its assigned coast station identification number (4 digits). Calls to ship stations must employ the following format: Ship station selective call number, repeated twice; “DE”, sent once; and coast station identification number, repeated twice. When...

47 CFR 80.121 - Public coast stations using telegraphy.

Code of Federal Regulations, 2011 CFR

2011-10-01

... use the ship station selective calling number (5 digits) and its assigned coast station identification number (4 digits). Calls to ship stations must employ the following format: Ship station selective call number, repeated twice; “DE”, sent once; and coast station identification number, repeated twice. When...

47 CFR 80.367 - General uses-radiotelephony.

Code of Federal Regulations, 2010 CFR

2010-10-01

... station. (b) Radiotelephony stations communicating with a Government station may transmit on a Government frequency when authorized to do so by the Government station or agency if the emission, bandwidth and frequency tolerance of the maritime station are within the same limits as the Government station. (c...

47 CFR 95.621 - GMRS transmitter channel frequencies.

Code of Federal Regulations, 2010 CFR

2010-10-01

... only for certain station classes and station locations. See part 95, subpart A. (b) Each GMRS transmitter for mobile station, small base station and control station operation must be maintained within a frequency tolerance of 0.0005%. Each GMRS transmitter for base station (except small base), mobile relay...

47 CFR 80.469 - Maritime mobile repeater stations in Alaska.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Maritime mobile repeater stations in Alaska. 80... RADIO SERVICES STATIONS IN THE MARITIME SERVICES Public Coast Stations Use of Telephony § 80.469 Maritime mobile repeater stations in Alaska. (a) Maritime mobile repeater stations are authorized to extend...

47 CFR 74.1233 - Processing FM translator and booster station applications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 4 2014-10-01 2014-10-01 false Processing FM translator and booster station... SERVICES FM Broadcast Translator Stations and FM Broadcast Booster Stations § 74.1233 Processing FM translator and booster station applications. (a) Applications for FM translator and booster stations are...

47 CFR 74.1233 - Processing FM translator and booster station applications.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 4 2012-10-01 2012-10-01 false Processing FM translator and booster station... SERVICES FM Broadcast Translator Stations and FM Broadcast Booster Stations § 74.1233 Processing FM translator and booster station applications. (a) Applications for FM translator and booster stations are...

47 CFR 74.1233 - Processing FM translator and booster station applications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 4 2011-10-01 2011-10-01 false Processing FM translator and booster station... SERVICES FM Broadcast Translator Stations and FM Broadcast Booster Stations § 74.1233 Processing FM translator and booster station applications. (a) Applications for FM translator and booster stations are...

47 CFR 74.1233 - Processing FM translator and booster station applications.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 4 2013-10-01 2013-10-01 false Processing FM translator and booster station... SERVICES FM Broadcast Translator Stations and FM Broadcast Booster Stations § 74.1233 Processing FM translator and booster station applications. (a) Applications for FM translator and booster stations are...

47 CFR 80.80 - Operating controls for ship stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Operating controls for ship stations. 80.80... SERVICES STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Station Requirements-Ship Stations § 80.80 Operating controls for ship stations. (a) Each control point must be capable of: (1...

47 CFR 97.211 - Space telecommand station.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Space telecommand station. 97.211 Section 97... AMATEUR RADIO SERVICE Special Operations § 97.211 Space telecommand station. (a) Any amateur station designated by the licensee of a space station is eligible to transmit as a telecommand station for that space...

47 CFR 97.211 - Space telecommand station.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Space telecommand station. 97.211 Section 97... AMATEUR RADIO SERVICE Special Operations § 97.211 Space telecommand station. (a) Any amateur station designated by the licensee of a space station is eligible to transmit as a telecommand station for that space...

47 CFR 97.211 - Space telecommand station.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Space telecommand station. 97.211 Section 97... AMATEUR RADIO SERVICE Special Operations § 97.211 Space telecommand station. (a) Any amateur station designated by the licensee of a space station is eligible to transmit as a telecommand station for that space...

47 CFR 97.211 - Space telecommand station.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Space telecommand station. 97.211 Section 97... AMATEUR RADIO SERVICE Special Operations § 97.211 Space telecommand station. (a) Any amateur station designated by the licensee of a space station is eligible to transmit as a telecommand station for that space...

47 CFR 97.211 - Space telecommand station.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Space telecommand station. 97.211 Section 97... AMATEUR RADIO SERVICE Special Operations § 97.211 Space telecommand station. (a) Any amateur station designated by the licensee of a space station is eligible to transmit as a telecommand station for that space...

47 CFR 80.80 - Operating controls for ship stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Operating controls for ship stations. 80.80... SERVICES STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Station Requirements-Ship Stations § 80.80 Operating controls for ship stations. (a) Each control point must be capable of: (1...

47 CFR 80.80 - Operating controls for ship stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Operating controls for ship stations. 80.80... SERVICES STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Station Requirements-Ship Stations § 80.80 Operating controls for ship stations. (a) Each control point must be capable of: (1...

47 CFR 80.80 - Operating controls for ship stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Operating controls for ship stations. 80.80... SERVICES STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Station Requirements-Ship Stations § 80.80 Operating controls for ship stations. (a) Each control point must be capable of: (1...

47 CFR 97.109 - Station control.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Station control. 97.109 Section 97.109... SERVICE Station Operation Standards § 97.109 Station control. (a) Each amateur station must have at least one control point. (b) When a station is being locally controlled, the control operator must be at the...

47 CFR 97.109 - Station control.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Station control. 97.109 Section 97.109... SERVICE Station Operation Standards § 97.109 Station control. (a) Each amateur station must have at least one control point. (b) When a station is being locally controlled, the control operator must be at the...

47 CFR 97.109 - Station control.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Station control. 97.109 Section 97.109... SERVICE Station Operation Standards § 97.109 Station control. (a) Each amateur station must have at least one control point. (b) When a station is being locally controlled, the control operator must be at the...

47 CFR 97.109 - Station control.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Station control. 97.109 Section 97.109... SERVICE Station Operation Standards § 97.109 Station control. (a) Each amateur station must have at least one control point. (b) When a station is being locally controlled, the control operator must be at the...

47 CFR 97.209 - Earth station.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Earth station. 97.209 Section 97.209... SERVICE Special Operations § 97.209 Earth station. (a) Any amateur station may be an Earth station. A holder of any class operator license may be the control operator of an Earth station, subject to the...

47 CFR 97.209 - Earth station.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Earth station. 97.209 Section 97.209... SERVICE Special Operations § 97.209 Earth station. (a) Any amateur station may be an Earth station. A holder of any class operator license may be the control operator of an Earth station, subject to the...

47 CFR 97.209 - Earth station.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Earth station. 97.209 Section 97.209... SERVICE Special Operations § 97.209 Earth station. (a) Any amateur station may be an Earth station. A holder of any class operator license may be the control operator of an Earth station, subject to the...

47 CFR 97.209 - Earth station.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Earth station. 97.209 Section 97.209... SERVICE Special Operations § 97.209 Earth station. (a) Any amateur station may be an Earth station. A holder of any class operator license may be the control operator of an Earth station, subject to the...

47 CFR 97.209 - Earth station.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Earth station. 97.209 Section 97.209... SERVICE Special Operations § 97.209 Earth station. (a) Any amateur station may be an Earth station. A holder of any class operator license may be the control operator of an Earth station, subject to the...

47 CFR 25.276 - Points of communication.

Code of Federal Regulations, 2010 CFR

2010-10-01

... authorization, an earth station is authorized to transmit to any space station in the same radio service provided that permission has been received from the space station operator to access that space station. (b) Space stations licensed under this part are authorized to provide service to earth stations located...

47 CFR 90.119 - Application requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... frequency, the station class, the total number of control stations, the emission, and the output power of... Services, including applications for new base, fixed, or mobile station authorizations governed by this part. (b) If the control station(s) will operate on the same frequency as the mobile station, and if...

47 CFR 90.119 - Application requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... frequency, the station class, the total number of control stations, the emission, and the output power of... Services, including applications for new base, fixed, or mobile station authorizations governed by this part. (b) If the control station(s) will operate on the same frequency as the mobile station, and if...

47 CFR 90.119 - Application requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... frequency, the station class, the total number of control stations, the emission, and the output power of... Services, including applications for new base, fixed, or mobile station authorizations governed by this part. (b) If the control station(s) will operate on the same frequency as the mobile station, and if...

47 CFR 73.1201 - Station identification.

Code of Federal Regulations, 2014 CFR

2014-10-01

...; Provided, That the name of the licensee, the station's frequency, the station's channel number, as stated... number in the station identification must use the station's major channel number and may distinguish multicast program streams. For example, a DTV station with major channel number 26 may use 26.1 to identify...

47 CFR 73.1201 - Station identification.

Code of Federal Regulations, 2013 CFR

2013-10-01

...; Provided, That the name of the licensee, the station's frequency, the station's channel number, as stated... number in the station identification must use the station's major channel number and may distinguish multicast program streams. For example, a DTV station with major channel number 26 may use 26.1 to identify...

47 CFR 90.119 - Application requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... frequency, the station class, the total number of control stations, the emission, and the output power of... Services, including applications for new base, fixed, or mobile station authorizations governed by this part. (b) If the control station(s) will operate on the same frequency as the mobile station, and if...

47 CFR 73.1201 - Station identification.

Code of Federal Regulations, 2011 CFR

2011-10-01

...; Provided, That the name of the licensee, the station's frequency, the station's channel number, as stated... number in the station identification must use the station's major channel number and may distinguish multicast program streams. For example, a DTV station with major channel number 26 may use 26.1 to identify...

47 CFR 73.1201 - Station identification.

Code of Federal Regulations, 2012 CFR

2012-10-01

...; Provided, That the name of the licensee, the station's frequency, the station's channel number, as stated... number in the station identification must use the station's major channel number and may distinguish multicast program streams. For example, a DTV station with major channel number 26 may use 26.1 to identify...

47 CFR 25.161 - Automatic termination of station authorization.

Code of Federal Regulations, 2014 CFR

2014-10-01

...(e) or, in the case of a space station license, an application for extension of the license term has... 47 Telecommunication 2 2014-10-01 2014-10-01 false Automatic termination of station authorization... Station Authorization § 25.161 Automatic termination of station authorization. A station authorization...

47 CFR 25.276 - Points of communication.

Code of Federal Regulations, 2011 CFR

2011-10-01

... authorization, an earth station is authorized to transmit to any space station in the same radio service provided that permission has been received from the space station operator to access that space station. (b) Space stations licensed under this part are authorized to provide service to earth stations located...