Study of Chemotaxis and Cell–Cell Interactions in Cancer with Microfluidic Devices

PubMed Central

Sai, Jiqing; Rogers, Matthew; Hockemeyer, Kathryn; Wikswo, John P.; Richmond, Ann

2017-01-01

Microfluidic devices have very broad applications in biological assays from simple chemotaxis assays to much more complicated 3D bioreactors. In this chapter, we describe the design and methods for performing chemotaxis assays using simple microfluidic chemotaxis chambers. With these devices, using real-time video microscopy we can examine the chemotactic responses of neutrophil-like cells under conditions of varying gradient steepness or flow rate and then utilize software programs to calculate the speed and angles of cell migration as gradient steepness and flow are varied. Considering the shearing force generated on the cells by the constant flow that is required to produce and maintain a stable gradient, the trajectories of the cell migration will reflect the net result of both shear force generated by flow and the chemotactic force resulting from the chemokine gradient. Moreover, the effects of mutations in chemokine receptors or the presence of inhibitors of intracellular signals required for gradient sensing can be evaluated in real time. We also describe a method to monitor intracellular signals required for cells to alter cell polarity in response to an abrupt switch in gradient direction. Lastly, we demonstrate an in vitro method for studying the interactions of human cancer cells with human endothelial cells, fibroblasts, and leukocytes, as well as environmental chemokines and cytokines, using 3D microbioreactors that mimic the in vivo microenvironment. PMID:26921940

Directional phytoscreening: contaminant gradients in trees for plume delineation.

PubMed

Limmer, Matt A; Shetty, Mikhil K; Markus, Samantha; Kroeker, Ryan; Parker, Beth L; Martinez, Camilo; Burken, Joel G

2013-08-20

Tree sampling methods have been used in phytoscreening applications to delineate contaminated soil and groundwater, augmenting traditional investigative methods that are time-consuming, resource-intensive, invasive, and costly. In the past decade, contaminant concentrations in tree tissues have been shown to reflect the extent and intensity of subsurface contamination. This paper investigates a new phytoscreening tool: directional tree coring, a concept originating from field data that indicated azimuthal concentrations in tree trunks reflected the concentration gradients in the groundwater around the tree. To experimentally test this hypothesis, large diameter trees were subjected to subsurface contaminant concentration gradients in a greenhouse study. These trees were then analyzed for azimuthal concentration gradients in aboveground tree tissues, revealing contaminant centroids located on the side of the tree nearest the most contaminated groundwater. Tree coring at three field sites revealed sufficiently steep contaminant gradients in trees reflected nearby groundwater contaminant gradients. In practice, trees possessing steep contaminant gradients are indicators of steep subsurface contaminant gradients, providing compass-like information about the contaminant gradient, pointing investigators toward higher concentration regions of the plume.

Integration of shallow gradients of Shh and Netrin-1 guides commissural axons.

PubMed

Sloan, Tyler F W; Qasaimeh, Mohammad A; Juncker, David; Yam, Patricia T; Charron, Frédéric

2015-03-01

During nervous system development, gradients of Sonic Hedgehog (Shh) and Netrin-1 attract growth cones of commissural axons toward the floor plate of the embryonic spinal cord. Mice defective for either Shh or Netrin-1 signaling have commissural axon guidance defects, suggesting that both Shh and Netrin-1 are required for correct axon guidance. However, how Shh and Netrin-1 collaborate to guide axons is not known. We first quantified the steepness of the Shh gradient in the spinal cord and found that it is mostly very shallow. We then developed an in vitro microfluidic guidance assay to simulate these shallow gradients. We found that axons of dissociated commissural neurons respond to steep but not shallow gradients of Shh or Netrin-1. However, when we presented axons with combined Shh and Netrin-1 gradients, they had heightened sensitivity to the guidance cues, turning in response to shallower gradients that were unable to guide axons when only one cue was present. Furthermore, these shallow gradients polarized growth cone Src-family kinase (SFK) activity only when Shh and Netrin-1 were combined, indicating that SFKs can integrate the two guidance cues. Together, our results indicate that Shh and Netrin-1 synergize to enable growth cones to sense shallow gradients in regions of the spinal cord where the steepness of a single guidance cue is insufficient to guide axons, and we identify a novel type of synergy that occurs when the steepness (and not the concentration) of a guidance cue is limiting.

Integration of Shallow Gradients of Shh and Netrin-1 Guides Commissural Axons

PubMed Central

Sloan, Tyler F. W.; Qasaimeh, Mohammad A.; Juncker, David; Yam, Patricia T.; Charron, Frédéric

2015-01-01

During nervous system development, gradients of Sonic Hedgehog (Shh) and Netrin-1 attract growth cones of commissural axons toward the floor plate of the embryonic spinal cord. Mice defective for either Shh or Netrin-1 signaling have commissural axon guidance defects, suggesting that both Shh and Netrin-1 are required for correct axon guidance. However, how Shh and Netrin-1 collaborate to guide axons is not known. We first quantified the steepness of the Shh gradient in the spinal cord and found that it is mostly very shallow. We then developed an in vitro microfluidic guidance assay to simulate these shallow gradients. We found that axons of dissociated commissural neurons respond to steep but not shallow gradients of Shh or Netrin-1. However, when we presented axons with combined Shh and Netrin-1 gradients, they had heightened sensitivity to the guidance cues, turning in response to shallower gradients that were unable to guide axons when only one cue was present. Furthermore, these shallow gradients polarized growth cone Src-family kinase (SFK) activity only when Shh and Netrin-1 were combined, indicating that SFKs can integrate the two guidance cues. Together, our results indicate that Shh and Netrin-1 synergize to enable growth cones to sense shallow gradients in regions of the spinal cord where the steepness of a single guidance cue is insufficient to guide axons, and we identify a novel type of synergy that occurs when the steepness (and not the concentration) of a guidance cue is limiting. PMID:25826604

Local patterns of biological N fixation and N-to-P limitations along steep atmospheric deposition gradients

NASA Astrophysics Data System (ADS)

Scott, Dalton; Bradley, Robert; Bellenger, Jean-Philippe; Kathrin, Rousk; Michael, Gundale; DeLuca, Tom

2017-04-01

A major challenge facing biogeochemists is being able to predict how environmental changes alter the functioning of forest ecosystems. In particular, atmospheric N deposition (AND) from fossil fuel combustion is fertilizing forest ecosystems worldwide at an unprecedented rate. While much attention has been paid to regional and continental-scale AND patterns, very little is known about local scale patterns resulting from human activities. For example, busy roads have recently been identified as hotspots for AND, with steep gradients occurring within 100-400 m margins along busy roadsides. It was previously found that such gradients along boreal forest roadsides correlated negatively with changes in biological N fixation (BNF) by moss dwelling cyanobacteria. Here, we present data from a recent experiment designed to answer specific questions regarding this phenomenon, namely: (1) Can AND lead to shifts from N to P limitation of BNF in mosses? (2) Can AND shift the stoichiometry of P and Mo (i.e. nitrogenase enzyme cofactor) limiting BNF in mosses? (3) Do roadside BNF patterns occur because of a down regulation in nitrogenase enzyme activity, or as the result of changes in moss biomass? (4) Do roadside AND and BNF patterns correlate predictably with the relative N-to-P limitation of trees? Preliminary results confirm that roadside BNF gradients are site specific, with moisture and light availability as major environmental controls. P-limitations of BNF were observed along roadside gradients on some sites, as were changes in spruce needle N and P concentrations. Decreases in BNF due to high AND may partly be due to changes in moss biomass. Collectively, our project provides important insights that improve our knowledge of site-specific stoichiometric gradients due to AND, which can be used to improve the precision of biogeochemical models required to predict ecosystem responses to global changes.

Light sheet microscopy reveals more gradual light attenuation in light green versus dark green soybean leaves

USDA-ARS?s Scientific Manuscript database

Light wavelengths preferentially absorbed by chlorophyll (chl) often display steep absorption gradients. This oversaturates photosynthesis in upper chloroplasts and deprives lower chloroplasts of blue and red light, causing a steep gradient in carbon fixation. Reducing chl content could create a mor...

Cell Blebbing in Confined Microfluidic Environments

PubMed Central

Ibo, Markela; Srivastava, Vasudha; Robinson, Douglas N.; Gagnon, Zachary R.

2016-01-01

Migrating cells can extend their leading edge by forming myosin-driven blebs and F-actin-driven pseudopods. When coerced to migrate in resistive environments, Dictyostelium cells switch from using predominately pseudopods to blebs. Bleb formation has been shown to be chemotactic and can be influenced by the direction of the chemotactic gradient. In this study, we determine the blebbing responses of developed cells of Dictyostelium discoideum to cAMP gradients of varying steepness produced in microfluidic channels with different confining heights, ranging between 1.7 μm and 3.8 μm. We show that microfluidic confinement height, gradient steepness, buffer osmolarity and Myosin II activity are important factors in determining whether cells migrate with blebs or with pseudopods. Dictyostelium cells were observed migrating within the confines of microfluidic gradient channels. When the cAMP gradient steepness is increased from 0.7 nM/μm to 20 nM/μm, cells switch from moving with a mixture of blebs and pseudopods to moving only using blebs when chemotaxing in channels with confinement heights less than 2.4 μm. Furthermore, the size of the blebs increases with gradient steepness and correlates with increases in myosin-II localization at the cell cortex. Reduction of intracellular pressure by high osmolarity buffer or inhibition of myosin-II by blebbistatin leads to a decrease in bleb formation and bleb size. Together, our data reveal that the protrusion type formed by migrating cells can be influenced by the channel height and the steepness of the cAMP gradient, and suggests that a combination of confinement-induced myosin-II localization and cAMP-regulated cortical contraction leads to increased intracellular fluid pressure and bleb formation. PMID:27706201

Adaptive Traits Are Maintained on Steep Selective Gradients despite Gene Flow and Hybridization in the Intertidal Zone

PubMed Central

Canovas, Fernando; Ferreira Costa, Joana; Serrão, Ester A.; Pearson, Gareth A.

2011-01-01

Gene flow among hybridizing species with incomplete reproductive barriers blurs species boundaries, while selection under heterogeneous local ecological conditions or along strong gradients may counteract this tendency. Congeneric, externally-fertilizing fucoid brown algae occur as distinct morphotypes along intertidal exposure gradients despite gene flow. Combining analyses of genetic and phenotypic traits, we investigate the potential for physiological resilience to emersion stressors to act as an isolating mechanism in the face of gene flow. Along vertical exposure gradients in the intertidal zone of Northern Portugal and Northwest France, the mid-low shore species Fucus vesiculosus, the upper shore species Fucus spiralis, and an intermediate distinctive morphotype of F. spiralis var. platycarpus were morphologically characterized. Two diagnostic microsatellite loci recovered 3 genetic clusters consistent with prior morphological assignment. Phylogenetic analysis based on single nucleotide polymorphisms in 14 protein coding regions unambiguously resolved 3 clades; sympatric F. vesiculosus, F. spiralis, and the allopatric (in southern Iberia) population of F. spiralis var. platycarpus. In contrast, the sympatric F. spiralis var. platycarpus (from Northern Portugal) was distributed across the 3 clades, strongly suggesting hybridization/introgression with both other entities. Common garden experiments showed that physiological resilience following exposure to desiccation/heat stress differed significantly between the 3 sympatric genetic taxa; consistent with their respective vertical distribution on steep environmental clines in exposure time. Phylogenetic analyses indicate that F. spiralis var. platycarpus is a distinct entity in allopatry, but that extensive gene flow occurs with both higher and lower shore species in sympatry. Experimental results suggest that strong selection on physiological traits across steep intertidal exposure gradients acts to maintain the 3 distinct genetic and morphological taxa within their preferred vertical distribution ranges. On the strength of distributional, genetic, physiological and morphological differences, we propose elevation of F. spiralis var. platycarpus from variety to species level, as F. guiryi. PMID:21695117

The role of selection and historical factors in driving population differentiation along an elevational gradient in an island bird.

PubMed

Bertrand, J A M; Delahaie, B; Bourgeois, Y X C; Duval, T; García-Jiménez, R; Cornuault, J; Pujol, B; Thébaud, C; Milá, B

2016-04-01

Adaptation to local environmental conditions and the range dynamics of populations can influence evolutionary divergence along environmental gradients. Thus, it is important to investigate patterns of both phenotypic and genetic variations among populations to reveal the respective roles of these two types of factors in driving population differentiation. Here, we test for evidence of phenotypic and genetic structure across populations of a passerine bird (Zosterops borbonicus) distributed along a steep elevational gradient on the island of Réunion. Using 11 microsatellite loci screened in 401 individuals from 18 localities distributed along the gradient, we found that genetic differentiation occurred at two spatial levels: (i) between two main population groups corresponding to highland and lowland areas, respectively, and (ii) within each of these two groups. In contrast, several morphological traits varied gradually along the gradient. Comparison of neutral genetic differentiation (FST ) and phenotypic differentiation (PST ) showed that PST largely exceeds FST at several morphological traits, which is consistent with a role for local adaptation in driving morphological divergence along the gradient. Overall, our results revealed an area of secondary contact midway up the gradient between two major, cryptic, population groups likely diverged in allopatry. Remarkably, local adaptation has shaped phenotypic differentiation irrespective of population history, resulting in different patterns of variation along the elevational gradient. Our findings underscore the importance of understanding both historical and selective factors when trying to explain variation along environmental gradients. © 2016 European Society For Evolutionary Biology. Journal of Evolutionary Biology © 2016 European Society For Evolutionary Biology.

Neutrophil migration under spatially-varying chemoattractant gradient profiles.

PubMed

Halilovic, Iris; Wu, Jiandong; Alexander, Murray; Lin, Francis

2015-01-01

Chemotaxis plays an important role in biological processes such as cancer metastasis, embryogenesis, wound healing, and immune response. Neutrophils are the frontline defenders against invasion of foreign microorganisms into our bodies. To achieve this important immune function, a neutrophil can sense minute chemoattractant concentration differences across its cell body and effectively migrate toward the chemoattractant source. Furthermore, it has been demonstrated in various studies that neutrophils are highly sensitive to changes in the surrounding chemoattractant environments, suggesting the role of a chemotactic memory for processing the complex spatiotemporal chemical guiding signals. Using a microfluidic device, in the present study we characterized neutrophil migration under spatially varying profiles of interleukine-8 gradients, which consist of three spatially ordered regions of a shallow gradient, a steep gradient and a nearly saturated gradient. This design allowed us to examine how neutrophils migrate under different chemoattractant gradient profiles, and how the migratory response is affected when the cell moves from one gradient profile to another in a single experiment. Our results show robust neutrophil chemotaxis in the shallow and steep gradient, but not the saturated gradient. Furthermore, neutrophils display a transition from chemotaxis to flowtaxis when they migrate across the steep gradient interface, and the relative efficiency of this transition depends on the cell's chemotaxis history. Finally, some neutrophils were observed to adjust their morphology to different gradient profiles.

Environmental Assessment for North Central Texas Relay Node Site NO. RN8C915TX

DTIC Science & Technology

1993-02-11

Relatively steep escarpments, reaching up to 20 percent in gradient , form boundaries between these incised river valleys and the surrounding plain...Pleistocene, approximately 11,500 to 7,500 years ago, when small bands composed of several families hunted megafauna such as bison and mammoth...conditions in the upper part Megafauna Large mammals such as bison and mammoths Native A generalized reference to an individual whose ancestry may be

Unveiling a phytoplankton hotspot at a narrow boundary between coastal and offshore waters

PubMed Central

Ribalet, Francois; Marchetti, Adrian; Hubbard, Katherine A.; Brown, Kristina; Durkin, Colleen A.; Morales, Rhonda; Robert, Marie; Swalwell, Jarred E.; Tortell, Philippe D.; Armbrust, E. Virginia

2010-01-01

In terrestrial ecosystems, transitional areas between different plant communities (ecotones) are formed by steep environmental gradients and are commonly characterized by high species diversity and primary productivity, which in turn influences the foodweb structure of these regions. Whether comparable zones of elevated diversity and productivity characterize ecotones in the oceans remains poorly understood. Here we describe a previously hidden hotspot of phytoplankton diversity and productivity in a narrow but seasonally persistent transition zone at the intersection of iron-poor, nitrate-rich offshore waters and iron-rich, nitrate-poor coastal waters of the Northeast Pacific Ocean. Novel continuous measurements of phytoplankton cell abundance and composition identified a complex succession of blooms of five distinct size classes of phytoplankton populations within a 100-km–wide transition zone. The blooms appear to be fueled by natural iron enrichment of offshore communities as they are transported toward the coast. The observed succession of phytoplankton populations is likely driven by spatial gradients in iron availability or time since iron enrichment. Regardless of the underlying mechanism, the resulting communities have a strong impact on the regional biogeochemistry as evidenced by the low partial pressure of CO2 and the nearly complete depletion of nutrients. Enhanced phytoplankton productivity and diversity associated with steep environmental gradients are expected wherever water masses with complementary nutrient compositions mix to create a region more favorable for phytoplankton growth. The ability to detect and track these important but poorly characterized marine ecotones is critical for understanding their impact on productivity and ecosystem structure in the oceans. PMID:20823224

Unveiling a phytoplankton hotspot at a narrow boundary between coastal and offshore waters.

PubMed

Ribalet, Francois; Marchetti, Adrian; Hubbard, Katherine A; Brown, Kristina; Durkin, Colleen A; Morales, Rhonda; Robert, Marie; Swalwell, Jarred E; Tortell, Philippe D; Armbrust, E Virginia

2010-09-21

In terrestrial ecosystems, transitional areas between different plant communities (ecotones) are formed by steep environmental gradients and are commonly characterized by high species diversity and primary productivity, which in turn influences the foodweb structure of these regions. Whether comparable zones of elevated diversity and productivity characterize ecotones in the oceans remains poorly understood. Here we describe a previously hidden hotspot of phytoplankton diversity and productivity in a narrow but seasonally persistent transition zone at the intersection of iron-poor, nitrate-rich offshore waters and iron-rich, nitrate-poor coastal waters of the Northeast Pacific Ocean. Novel continuous measurements of phytoplankton cell abundance and composition identified a complex succession of blooms of five distinct size classes of phytoplankton populations within a 100-km-wide transition zone. The blooms appear to be fueled by natural iron enrichment of offshore communities as they are transported toward the coast. The observed succession of phytoplankton populations is likely driven by spatial gradients in iron availability or time since iron enrichment. Regardless of the underlying mechanism, the resulting communities have a strong impact on the regional biogeochemistry as evidenced by the low partial pressure of CO(2) and the nearly complete depletion of nutrients. Enhanced phytoplankton productivity and diversity associated with steep environmental gradients are expected wherever water masses with complementary nutrient compositions mix to create a region more favorable for phytoplankton growth. The ability to detect and track these important but poorly characterized marine ecotones is critical for understanding their impact on productivity and ecosystem structure in the oceans.

Drift and Behavior of E. coli Cells

NASA Astrophysics Data System (ADS)

Micali, Gabriele; Colin, Rémy; Sourjik, Victor; Endres, Robert G.

2017-12-01

Chemotaxis of the bacterium Escherichia coli is well understood in shallow chemical gradients, but its swimming behavior remains difficult to interpret in steep gradients. By focusing on single-cell trajectories from simulations, we investigated the dependence of the chemotactic drift velocity on attractant concentration in an exponential gradient. While maxima of the average drift velocity can be interpreted within analytical linear-response theory of chemotaxis in shallow gradients, limits in drift due to steep gradients and finite number of receptor-methylation sites for adaptation go beyond perturbation theory. For instance, we found a surprising pinning of the cells to the concentration in the gradient at which cells run out of methylation sites. To validate the positions of maximal drift, we recorded single-cell trajectories in carefully designed chemical gradients using microfluidics.

Water resources of the River Rouge basin southeastern Michigan

USGS Publications Warehouse

Knutilla, R.L.

1971-01-01

The River Rouge basin is characterized by moderately hilly topography to the northwest graduating to a relatively level land surface to the south east.Stream gradients near the northwestern basin divide are relatively steep; but many become more steep in reaches where they cross beach lines of former glacial lakes. In the lower reaches of the River Rouge gradients lessen.

Range-Wide Latitudinal and Elevational Temperature Gradients for the World's Terrestrial Birds: Implications under Global Climate Change

PubMed Central

La Sorte, Frank A.; Butchart, Stuart H. M.; Jetz, Walter; Böhning-Gaese, Katrin

2014-01-01

Species' geographical distributions are tracking latitudinal and elevational surface temperature gradients under global climate change. To evaluate the opportunities to track these gradients across space, we provide a first baseline assessment of the steepness of these gradients for the world's terrestrial birds. Within the breeding ranges of 9,014 bird species, we characterized the spatial gradients in temperature along latitude and elevation for all and a subset of bird species, respectively. We summarized these temperature gradients globally for threatened and non-threatened species and determined how their steepness varied based on species' geography (range size, shape, and orientation) and projected changes in temperature under climate change. Elevational temperature gradients were steepest for species in Africa, western North and South America, and central Asia and shallowest in Australasia, insular IndoMalaya, and the Neotropical lowlands. Latitudinal temperature gradients were steepest for extratropical species, especially in the Northern Hemisphere. Threatened species had shallower elevational gradients whereas latitudinal gradients differed little between threatened and non-threatened species. The strength of elevational gradients was positively correlated with projected changes in temperature. For latitudinal gradients, this relationship only held for extratropical species. The strength of latitudinal gradients was better predicted by species' geography, but primarily for extratropical species. Our findings suggest threatened species are associated with shallower elevational temperature gradients, whereas steep latitudinal gradients are most prevalent outside the tropics where fewer bird species occur year-round. Future modeling and mitigation efforts would benefit from the development of finer grain distributional data to ascertain how these gradients are structured within species' ranges, how and why these gradients vary among species, and the capacity of species to utilize these gradients under climate change. PMID:24852009

Ecological and morphological traits predict depth-generalist fishes on coral reefs

PubMed Central

Bridge, Tom C. L.; Luiz, Osmar J.; Coleman, Richard R.; Kane, Corinne N.; Kosaki, Randall K.

2016-01-01

Ecological communities that occupy similar habitats may exhibit functional convergence despite significant geographical distances and taxonomic dissimilarity. On coral reefs, steep gradients in key environmental variables (e.g. light and wave energy) restrict some species to shallow depths. We show that depth-generalist reef fishes are correlated with two species-level traits: caudal fin aspect ratio and diet. Fishes with high aspect ratio (lunate) caudal fins produce weaker vortices in the water column while swimming, and we propose that ‘silent swimming’ reduces the likelihood of detection and provides an advantage on deeper reefs with lower light irradiance and water motion. Significant differences in depth preference among trophic guilds reflect variations in the availability of different food sources along a depth gradient. The significance of these two traits across three geographically and taxonomically distinct assemblages suggests that deep-water habitats exert a strong environmental filter on coral reef-fish assemblages. PMID:26791616

Historical Contingencies in Microbial Responses to Drought

NASA Astrophysics Data System (ADS)

Hawkes, C.; Waring, B.; Rocca, J.; Kivlin, S.; Giauque, H.; Averill, C.

2014-12-01

Although water is a primary controller of microbial function and we expect climate change to alter water availability in the future, our understanding of how microbial communities respond to a change in moisture and what that means for soil carbon cycling remain poorly understood. In part, this uncertainty arises from a lack of understanding of microbial response mechanisms and how those lead to aggregate soil function. Environmental tracking would be facilitated if microbial communities respond to new climatic conditions via rapid physiological acclimatization, shifts in community composition, or adaptation. In contrast, historical contingencies could be created by dispersal limitation or local adaptation to previous conditions. To address environmental tracking vs. legacies, we examined how soil microbial communities were affected by precipitation at multiple scales and asked whether rainfall was a primary driver of the observed responses. We leveraged a local steep rainfall gradient with field surveys, lab incubations, reciprocal transplants, and rainfall manipulations to approach this problem. Across a steep rainfall gradient, we found that soil microbial communities were strongly associated with historical rainfall, with two-thirds of the variation in community composition explained by mean annual precipitation. In 12-month experimental lab manipulations of soil moisture, soil functional responses were constrained by historical rainfall, with greater activity in soils subjected to their original moisture condition. The constraints of historical rainfall held even after 18 months in reciprocal transplant common gardens along the rainfall gradient and with manipulated dispersal of regional microbial communities. Yet, when water was manipulated at a single site over 4 years, legacies did not develop. Overall, these findings are consistent with long-term rainfall acting as a strong habitat filter and resulting in a legacy of both microbial community composition and physiological capacity that can affect soil carbon cycling. Placing the ecological and evolutionary dynamics of microbial communities in the context of historical and future environmental variation may thus provide us with a framework for improving prediction of ecosystem responses to climate change.

Combined solvent- and non-uniform temperature-programmed gradient liquid chromatography. I - A theoretical investigation.

PubMed

Gritti, Fabrice

2016-11-18

An new class of gradient liquid chromatography (GLC) is proposed and its performance is analyzed from a theoretical viewpoint. During the course of such gradients, both the solvent strength and the column temperature are simultaneously changed in time and space. The solvent and temperature gradients propagate along the chromatographic column at their own and independent linear velocity. This class of gradient is called combined solvent- and temperature-programmed gradient liquid chromatography (CST-GLC). The general expressions of the retention time, retention factor, and of the temporal peak width of the analytes at elution in CST-GLC are derived for linear solvent strength (LSS) retention models, modified van't Hoff retention behavior, linear and non-distorted solvent gradients, and for linear temperature gradients. In these conditions, the theory predicts that CST-GLC is equivalent to a unique and apparent dynamic solvent gradient. The apparent solvent gradient steepness is the sum of the solvent and temperature steepness. The apparent solvent linear velocity is the reciprocal of the steepness-averaged sum of the reciprocal of the actual solvent and temperature linear velocities. The advantage of CST-GLC over conventional GLC is demonstrated for the resolution of protein digests (peptide mapping) when applying smooth, retained, and linear acetonitrile gradients in combination with a linear temperature gradient (from 20°C to 90°C) using 300μm×150mm capillary columns packed with sub-2 μm particles. The benefit of CST-GLC is demonstrated when the temperature gradient propagates at the same velocity as the chromatographic speed. The experimental proof-of-concept for the realization of temperature ramps propagating at a finite and constant linear velocity is also briefly described. Copyright © 2016 Elsevier B.V. All rights reserved.

Steep, Transient Density Gradients in the Martian Ionosphere Similar to the Ionopause at Venus

NASA Astrophysics Data System (ADS)

Duru, Firdevs; Gurnett, Donald; Frahm, Rudy; Winningham, D. L.; Morgan, David; Howes, Gregory

Using Mars Advanced Radar for Subsurface and Ionospheric Sounding (MARSIS) on the Mars Express (MEX) spacecraft, the electron density can be measured by two methods: from the excitation of local plasma oscillations and from remote sounding. A study of the local electron density versus time for 1664 orbits revealed that in 132 orbits very sharp gradients in the electron density occurred that are similar to the ionopause boundary commonly observed at Venus. In 40 of these cases, remote sounding data have also confirmed identical locations of steep ionopause-like density gradients. Measurements from the Analyzer of Space Plasma and Energetic Atoms (ASPERA-3) Electron Spectrometer (ELS) and Ion Mass Analyzer (IMA) instruments (also on Mars Express) verify that these sharp decreases in the electron density occur somewhere between the end of the region where ionospheric photoelectrons are dominant and the magnetosheath. Combined studies of the two experiments reveal that the steep density gradients define a boundary where the magnetic fields change from open to closed. This study shows that, although the individual cases are from a wide range of altitudes, the average altitude of the boundary as a function of solar zenith angle is almost constant. The average altitude is approximately 500 km up to solar zenith angles of 60o, after which it shows a slight increase. The average thickness of the boundary is about 22 km according to remote sounding measurements. The altitude of the steep gradients shows an increase at locations with strong crustal magnetic fields.

The metallicity and elemental abundance gradients of simulated galaxies and their environmental dependence

NASA Astrophysics Data System (ADS)

Taylor, Philip; Kobayashi, Chiaki

2017-11-01

The internal distribution of heavy elements, in particular the radial metallicity gradient, offers insight into the merging history of galaxies. Using our cosmological, chemodynamical simulations that include both detailed chemical enrichment and feedback from active galactic nuclei (AGN), we find that stellar metallicity gradients in the most massive galaxies (≳3 × 1010M⊙) are made flatter by mergers and are unable to regenerate due to the quenching of star formation by AGN feedback. The fitting range is chosen on a galaxy-by-galaxy basis in order to mask satellite galaxies. The evolutionary paths of the gradients can be summarized as follows: (I) creation of initial steep gradients by gas-rich assembly, (II) passive evolution by star formation and/or stellar accretion at outskirts, and (III) sudden flattening by mergers. There is a significant scatter in gradients at a given mass, which originates from the last path, and therefore from galaxy type. Some variation remains at given galaxy mass and type because of the complexity of merging events, and hence we find only a weak environmental dependence. Our early-type galaxies (ETGs), defined from the star formation main sequence rather than their morphology, are in excellent agreement with the observed stellar metallicity gradients of ETGs in the SAURON and ATLAS3D surveys. We find small positive [O/Fe] gradients of stars in our simulated galaxies, although they are smaller with AGN feedback. Gas-phase metallicity and [O/Fe] gradients also show variation, the origin of which is not as clear as for stellar populations.

Monolithic stationary phases with a longitudinal gradient of porosity.

PubMed

Urban, Jiří; Hájek, Tomáš; Svec, Frantisek

2017-04-01

The duration of the hypercrosslinking reaction has been used to control the extent of small pores formation in polymer-based monolithic stationary phases. Segments of five columns hypercrosslinked for 30-360 min were coupled via zero-volume unions to prepare columns with segmented porosity gradients. The steepness of the porosity gradient affected column efficiency, mass transfer resistance, and separation of both small-molecule alkylbenzenes and high-molar-mass polystyrene standards. In addition, the segmented column with the steepest porosity gradient was prepared as a single column with a continuous porosity gradient. The steepness of porosity gradient in this type column was tuned. Compared to a completely hypercrosslinked column, the column with the shallower gradient produced comparable size-exclusion separation of polystyrene standards but allowed higher column permeability. The completely hypercrosslinked column and the column with porosity gradient were successfully coupled in online two-dimensional liquid chromatography of polymers. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Adaptation to a steep environmental gradient and an associated barrier to gene exchange in Littorina saxatilis.

PubMed

Grahame, John W; Wilding, Craig S; Butlin, Roger K

2006-02-01

Steep environmental gradients offer important opportunities to study the interaction between natural selection and gene flow. Allele frequency clines are expected to form at loci under selection, but unlinked neutral alleles may pass easily across these clines unless a generalized barrier evolves. Here we consider the distribution of forms of the intertidal gastropod Littorina saxatilis, analyzing shell shape and amplified fragment length polymorphism (AFLP) loci on two rocky shores in Britain. On the basis of previous work, the AFLP loci were divided into differentiated and undifferentiated groups. On both shores, we have shown a sharp cline in allele frequencies between the two morphs for differentiated AFLP loci. This is coincident with a habitat transition on the shore where the two habitats (cliff and boulder field) are immediately contiguous. The allele frequency clines coincide with a cline in shell morphology. In the middle of the cline, linkage disequilibrium for the differentiated loci rises in accordance with expectation. The clines are extremely narrow relative to dispersal, probably as a result of both strong selection and habitat choice. An increase in F(ST) for undifferentiated AFLPs between morphs, relative to within-morph comparisons, is consistent with there being a general barrier to gene flow across the contact zone. These features are consistent either with an episode of allopatric divergence followed by secondary contact or with primary, nonallopatric divergence. Further data will be needed to distinguish between these alternatives.

Source-to-sink sediment transfers, environmental engineering and hazard mitigation in the steep Var River catchment, French Riviera, southeastern France

NASA Astrophysics Data System (ADS)

Anthony, Edward J.; Julian, Maurice

1999-12-01

Steep coastal margins are potentially subject to mass wasting processes involving notable landslide activity and sediment evacuation downstream by steep-gradient streams. Sediment transfer from short source-to-sink segments, coupled with mountain hydrological regimes, regulate patterns of river channel aggradation and coastal sediment supply in such geomorphic settings. On the steep French Riviera margin, sediment transfers from existing landslides or from various minor mass wasting processes to stream channels may result following bursts of heavy, concentrated rainfall. High-magnitude flooding and massive sediment transport downstream are generally related to unpredictable extreme rainfalls. Both mass movements and channel sediment storage pose serious hazards to downvalley settlements and infrastructure. A consideration of channel sediment storage patterns in the Var River catchment, the most important catchment in this area, highlights two important shortcomings relative to environmental engineering and hazard mitigation practices. In the first place, the appreciation of geomorphic processes is rather poor. This is illustrated by the undersized nature of engineering works constructed to mitigate hazards in the upstream bedload-dominated channels, and by the unforeseen effects that ten rock dams, constructed in the early 1970s, have had on downstream and coastal sediment storage and on sediment dispersal patterns and, consequently, valley flooding. Secondly, planners and environmental engineers have lacked foresight in valley and coastal management issues on this steep setting, notably as regards the reclaimed areas of the lower Var channel and delta liable to flooding. Urbanization and transport and environmental engineering works have progressively affected patterns of storage and transport of fine-grained sediments in the lower Var channel and delta. Meanwhile the problems raised by these changes have not been adequately addressed in terms of scientific research. A necessary future step in bettering the engineering solutions implemented to contain natural hazards or to harness water and sediment resources is that of fine-scale analysis of source-to-sink sediment transfer processes, of sediment budgets, of time-scales of storage in stream channels, and, finally, of high-magnitude hydrometeorological forcing events in this area. The way all these aspects have been modulated by engineering practices and socioeconomic development should also be an important part of such an analysis.

Fluvial signatures of modern and paleo orographic rainfall gradients

NASA Astrophysics Data System (ADS)

Schildgen, Taylor; Strecker, Manfred

2016-04-01

The morphology of river profiles is intimately linked to both climate and tectonic forcing. While much interest recently has focused on how river profiles can be inverted to derive uplift histories, here we show how in regions of strong orographic rainfall gradients, rivers may primarily record spatial patterns of precipitation. As a case study, we examine the eastern margin of the Andean plateau in NW Argentina, where the outward (eastward) growth of a broken foreland has led to a eastward shift in the main orographic rainfall gradient over the last several million years. Rivers influenced by the modern rainfall gradient are characterized by normalized river steepness values in tributary valleys that closely track spatial variations in rainfall, with higher steepness values in drier areas and lower steepness values in wetter areas. The same river steepness pattern has been predicted in landscape evolution models that apply a spatial gradient in rainfall to a region of uniform erosivity and uplift rate (e.g., Han et al., 2015). Also, chi plots from river networks on individual ranges affected by the modern orographic rainfall reveal patterns consistent with assymmetric precipitation across the range: the largest channels on the windward slopes are characterized by capture, while the longest channels on the leeward slopes are dominated by beheadings. Because basins on the windward side both lengthen and widen, tributary channels in the lengthening basins are characterized by capture, while tributary channels from neighboring basins on the windward side are dominated by beheadings. These patterns from the rivers influenced by the modern orographic rainfall gradient provide a guide for identifying river morphometric signatures of paleo orographic rainfall gradients. Mountain ranges to the west of the modern orographic rainfall have been interpreted to mark the location of orographic rainfall in the past, but these ranges are now in spatially near-uniform semi-arid to arid precipitation regimes. Indeed, despite uniform lithology and uplift history, we see patterns in river steepness values and in chi plots that are consistest a rainfall gradient on the (former) windward side of the range and asymmetric precipitation across the range. We suggest that morphological aspects of the river networks in such regions are dominated by their history of changing climate. These morphologic signatures appear to persist for millions of years in NW Argentina, most likely because the transition from a wetter to a drier climate has prevented a rapid readjustment to new forcing conditions. Reference: Han, J., Gasparini, N.M., and Johnson, J.P., 2015, Measuring the imprint of orographic rainfall gradients on the morphology of steady-state numerical fluvial landscapes. Earth Surf. Process. Landforms, 40(10), 1334-1350.

Restoring Wood-Rich Hotspots in Mountain Stream Networks

NASA Astrophysics Data System (ADS)

Wohl, E.; Scott, D.

2016-12-01

Mountain streams commonly include substantial longitudinal variability in valley and channel geometry, alternating repeatedly between steep, narrow and relatively wide, low gradient segments. Segments that are wider and lower gradient than neighboring steeper sections are hotspots with respect to: retention of large wood (LW) and finer sediment and organic matter; uptake of nutrients; and biomass and biodiversity of aquatic and riparian organisms. These segments are also more likely to be transport-limited with respect to floodplain and instream LW. Management designed to protect and restore riverine LW and the physical and ecological processes facilitated by the presence of LW is likely to be most effective if focused on relatively low-gradient stream segments. These segments can be identified using a simple, reach-scale gradient analysis based on high-resolution DEMs, with field visits to identify factors that potentially limit or facilitate LW recruitment and retention, such as forest disturbance history or land use. Drawing on field data from the western US, this presentation outlines a procedure for mapping relatively low-gradient segments in a stream network and for identifying those segments where LW reintroduction or retention is most likely to balance maximizing environmental benefits derived from the presence of LW while minimizing hazards associated with LW.

Numerical solution of transport equation for applications in environmental hydraulics and hydrology

NASA Astrophysics Data System (ADS)

Rashidul Islam, M.; Hanif Chaudhry, M.

1997-04-01

The advective term in the one-dimensional transport equation, when numerically discretized, produces artificial diffusion. To minimize such artificial diffusion, which vanishes only for Courant number equal to unity, transport owing to advection has been modeled separately. The numerical solution of the advection equation for a Gaussian initial distribution is well established; however, large oscillations are observed when applied to an initial distribution with sleep gradients, such as trapezoidal distribution of a constituent or propagation of mass from a continuous input. In this study, the application of seven finite-difference schemes and one polynomial interpolation scheme is investigated to solve the transport equation for both Gaussian and non-Gaussian (trapezoidal) initial distributions. The results obtained from the numerical schemes are compared with the exact solutions. A constant advective velocity is assumed throughout the transport process. For a Gaussian distribution initial condition, all eight schemes give excellent results, except the Lax scheme which is diffusive. In application to the trapezoidal initial distribution, explicit finite-difference schemes prove to be superior to implicit finite-difference schemes because the latter produce large numerical oscillations near the steep gradients. The Warming-Kutler-Lomax (WKL) explicit scheme is found to be better among this group. The Hermite polynomial interpolation scheme yields the best result for a trapezoidal distribution among all eight schemes investigated. The second-order accurate schemes are sufficiently accurate for most practical problems, but the solution of unusual problems (concentration with steep gradient) requires the application of higher-order (e.g. third- and fourth-order) accurate schemes.

Adaptive microfluidic gradient generator for quantitative chemotaxis experiments.

PubMed

Anielski, Alexander; Pfannes, Eva K B; Beta, Carsten

2017-03-01

Chemotactic motion in a chemical gradient is an essential cellular function that controls many processes in the living world. For a better understanding and more detailed modelling of the underlying mechanisms of chemotaxis, quantitative investigations in controlled environments are needed. We developed a setup that allows us to separately address the dependencies of the chemotactic motion on the average background concentration and on the gradient steepness of the chemoattractant. In particular, both the background concentration and the gradient steepness can be kept constant at the position of the cell while it moves along in the gradient direction. This is achieved by generating a well-defined chemoattractant gradient using flow photolysis. In this approach, the chemoattractant is released by a light-induced reaction from a caged precursor in a microfluidic flow chamber upstream of the cell. The flow photolysis approach is combined with an automated real-time cell tracker that determines changes in the cell position and triggers movement of the microscope stage such that the cell motion is compensated and the cell remains at the same position in the gradient profile. The gradient profile can be either determined experimentally using a caged fluorescent dye or may be alternatively determined by numerical solutions of the corresponding physical model. To demonstrate the function of this adaptive microfluidic gradient generator, we compare the chemotactic motion of Dictyostelium discoideum cells in a static gradient and in a gradient that adapts to the position of the moving cell.

Trail impacts in Sagarmatha (Mt. Everest) National Park, Nepal: a logistic regression analysis.

PubMed

Nepal, S K

2003-09-01

A trail study was conducted in the Sagarmatha (Mt. Everest) National Park, Nepal, during 1997-1998. Based on that study, this paper examines the spatial variability of trail conditions and analyzes factors that influence trail conditions. Logistic regression (multinomial logit model) is applied to examine the influence of use and environmental factors on trail conditions. The assessment of trail conditions is based on a four-class rating system: (class I, very little damaged; class II, moderately damaged, class III, heavily damaged; and class IV, severely damaged). Wald statistics and a model classification table have been used for data interpretation. Results indicate that altitude, trail gradient, hazard potential, and vegetation type are positively associated with trail condition. Trails are more degraded at higher altitude, on steep gradients, in areas with natural hazard potential, and within shrub/grassland zones. Strong correlations between high levels of trail degradation and higher frequencies of visitors and lodges were found. A detailed analysis of environmental and use factors could provide valuable information to park managers in their decisions about trail design, layout and maintenance, and efficient and effective visitor management strategies. Comparable studies on high alpine environments are needed to predict precisely the effects of topographic and climatic extremes. More refined approaches and experimental methods are necessary to control the effects of environmental factors.

Radial deformation of the solar current sheet as a cause of geomagnetic storms

NASA Technical Reports Server (NTRS)

Akasofu, S.-I.

1979-01-01

It is suggested that the solar current sheet, extending from a coronal streamer, develops a large-scale radial deformation, at times with a very steep gradient at the earth's distance. The associated magnetic field lines (namely, the interplanetary magnetic field (IMF) lines) are expected to have also a large gradient in the vicinity of the current sheet. It is also suggested that some of the major geomagnetic storms occur when the earth is located in the region where IMF field lines have a large dip angle with respect to the ecliptic plane for an extended period (6-48 h), as a result of a steep radial deformation of the current sheet.

Topographic and ecological controls on root reinforcement

Treesearch

T.C. Hales; C.R. Ford; T. Hwang; J.M. Vose; L.E. Band

2009-01-01

Shallow landslides are a significant hazard in steep, soil-mantled landscapes. During intense rainfall events, the distribution of shallow landslides is controlled by variations in landscape gradient, the frictional and cohesive properties of soil and roots, and the subsurface hydrologic response. While gradients can be estimated from digital elevation models,...

Topographic and ecologic controls on root reinforcement

Treesearch

T.C. Hales; C.R. Ford; T. Hwang; J.M. Vose; L.E. Band

2009-01-01

Shallow landslides are a significant hazard in steep, soil-mantled landscapes. During intense rainfall events, the distribution of shallow landslides is controlled by variations in landscape gradient, the frictional and cohesive properties of soil and roots, and the subsurface hydrologic response. While gradients can be estimated from digital elevation models,...

Centimeter-scale characterization of biogeochemical gradients at a wetland-aquifer interface using capillary electrophoresis

USGS Publications Warehouse

Baez-Cazull, S.; McGuire, J.T.; Cozzarelli, I.M.; Raymond, A.; Welsh, L.

2007-01-01

Steep biogeochemical gradients were measured at mixing interfaces in a wetland-aquifer system impacted by landfill leachate in Norman, Oklahoma. The system lies within a reworked alluvial plain and is characterized by layered low hydraulic conductivity wetland sediments interbedded with sandy aquifer material. Using cm-scale passive diffusion samplers, "peepers", water samples were collected in a depth profile to span interfaces between surface water and a sequence of deeper sedimentary layers. Geochemical indicators including electron acceptors, low-molecular-weight organic acids, base cations, and NH4+ were analyzed by capillary electrophoresis (CE) and field techniques to maximize the small sample volumes available from the centimeter-scale peepers. Steep concentration gradients of biogeochemical indicators were observed at various interfaces including those created at sedimentary boundaries and boundaries created by heterogeneities in organic C and available electron acceptors. At the sediment-water interface, chemical profiles with depth suggest that SO42 - and Fe reduction dominate driven by inputs of organic C from the wetland and availability of electron acceptors. Deeper in the sediments (not associated with a lithologic boundary), a steep gradient of organic acids (acetate maximum 8.8 mM) and NH4+ (maximum 36 mM) is observed due to a localized source of organic matter coupled with the lack of electron acceptor inputs. These findings highlight the importance of quantifying the redox reactions occurring in small interface zones and assessing their role on biogeochemical cycling at the system scale. ?? 2007 Elsevier Ltd. All rights reserved.

Fault-dominated deformation in an ice dam during annual filling and drainage of a marginal lake

USGS Publications Warehouse

Walder, J.S.; Trabant, D.C.; Cunico, M.; Anderson, S.P.; Anderson, R. Scott; Fountain, A.G.; Malm, A.

2005-01-01

Ice-dammed Hidden Creek Lake, Alaska, USA, outbursts annually in about 2-3 days. As the lake fills, a wedge of water penetrates beneath the glacier, and the surface of this 'ice dam' rises; the surface then falls as the lake drains. Detailed optical surveying of the glacier near the lake allows characterization of ice-dam deformation. Surface uplift rate is close to the rate of lake-level rise within about 400 m of the lake, then decreases by 90% over about 100 m. Such a steep gradient in uplift rate cannot be explained in terms of ice-dam flexure. Moreover, survey targets spanning the zone of steep uplift gradient move relative to one another in a nearly reversible fashion as the lake fills and drains. Evidently, the zone of steep uplift gradient is a fault zone, with the faults penetrating the entire thickness of the ice dam. Fault motion is in a reverse sense as the lake fills, but in a normal sense as the lake drains. As the overall fault pattern is the same from year to year, even though ice is lost by calving, the faults must be regularly regenerated, probably by linkage of surface and bottom crevasses as ice is advected toward the lake basin.

Landslides control the spatial and temporal variation of channel width in southern Taiwan: implications for landscape evolution and cascading hazards in steep, tectonically active landscapes

NASA Astrophysics Data System (ADS)

Yanites, B.; Bregy, J. C.; Carlson, G.; Cataldo, K.; Holahan, M.; Johnston, G.; Mitchell, N. A.; Nelson, A.; Valenza, J.; Wanker, M.

2017-12-01

Intense precipitation or seismic events can generate clustered mass movement processes across a landscape. These rare events have significant impacts on the landscape, however, the rarity of such events leads to uncertainty in how these events impact the entire geomorphic system over a range of timescales. Taiwan is a steep, seismically active region and is highly prone to landslide and debris flows, especially when exposed to heavy rainfall events. Typhoon Morakot made landfall in Taiwan in August of 2009, delivering record-breaking rainfall and inducing more than 22,000 landslides in southern Taiwan. The topographic gradient in southern Taiwan leads to spatial variability in landslide susceptibility providing an opportunity to infer the long-term impact of landslides on channel morphology. The availability of pre and post typhoon imagery allows a quantitative reconstruction on the propagating impact of this event on channel width. The pre and post typhoon patterns of channel width to river and hillslope gradients in 20 basins in the study area reveal the importance of cascading hazards from landslides on landscape evolution. Prior to Typhoon Morakot, the river channels in the central part of the study area were about 3-10 times wider than the channels in the south. Aggradation and widening was also a maximum in these basins where hillslope gradients and channel steepness is high. The results further show that the narrowest channels are located where channel steepness is the lowest, an observation inconsistent with a detachment-limited model for river evolution. We infer this pattern is indicative of a strong role of sediment supply, and associated landslide events, on long-term channel evolution. These findings have implications across a range of spatial and temporal scales including understanding the cascade of hazards in steep landscapes and geomorphic interpretation of channel morphology.

Landscape-scale processes influence riparian plant composition along a regulated river

USGS Publications Warehouse

Palmquist, Emily C.; Ralston, Barbara; Merritt, David M.; Shafroth, Patrick B.

2018-01-01

Hierarchical frameworks are useful constructs when exploring landscape- and local-scale factors affecting patterns of vegetation in riparian areas. In drylands, which have steep environmental gradients and high habitat heterogeneity, landscape-scale variables, such as climate, can change rapidly along a river's course, affecting the relative influence of environmental variables at different scales. To assess how landscape-scale factors change the structure of riparian vegetation, we measured riparian vegetation composition along the Colorado River through Grand Canyon, determined which factors best explain observed changes, identified how richness and functional diversity vary, and described the implications of our results for river management. Cluster analysis identified three divergent floristic groups that are distributed longitudinally along the river. These groups were distributed along gradients of elevation, temperature and seasonal precipitation, but were not associated with annual precipitation or local-scale factors. Species richness and functional diversity decreased as a function of distance downstream showing that changing landscape-scale factors result in changes to ecosystem characteristics. Species composition and distribution remain closely linked to seasonal precipitation and temperature. These patterns in floristic composition in a semiarid system inform management and provide insights into potential future changes as a result of shifts in climate and changes in flow management.

Unravelling environmental conditions during the Holocene in the Dead Sea region using multiple archives

NASA Astrophysics Data System (ADS)

Rambeau, Claire; van Leeuwen, Jacqueline; van der Knaap, Pim; Gobet, Erika

2016-04-01

For the most arid parts of the Southern Levant (roughly corresponding to modern Jordan, Israel and Palestine), environmental reconstructions are impeded by the limited number of archives, and the frequent contradictions between individual palaeoenvironmental records. The Southern Levant is characterised by steep climate gradients; local conditions presently range from arid to dry Mediterranean, with limits that may have fluctuated during the Holocene. This further complicates the determination of site-specific past environmental conditions. Understanding past climate and environmental evolution through time, at a local level, is however crucial to compare these with societal evolution during the Holocene, which features major cultural developments such as cereal cultivation, animal domestication, water management, as well as times of preferential settlement growth or site abandonment. This contribution proposes to examine the different archives available for the Dead Sea region, paying special attention to the most recent pollen data obtained from the area. It will particularly critically compare local to regional-scale information, and try to decipher the main evolutions of environmental conditions during the Holocene in arid and semi-arid Southern Levant.

Vegetation-environment relationships in zero-order basins in coastal Oregon.

Treesearch

Chris D. Sheridan; Thomas A. Spies

2005-01-01

Zero-order basins, where hillslope topography converges to form drainages, are common in steep, forested landscapes but we know little about their ecological structure. We used indirect gradient analysis to characterize gradients in plant species composition and cluster analysis to characterize groups of plant species associated with specific geomorphic areas. We...

Phylogenetic diversity of macromycetes and woody plants along an elevational gradient in Eastern Mexico

Treesearch

Marko Gomez-Hernandez; Guadalupe Williams-Linera; D. Jean Lodge; Roger Guevara; Eduardo Ruiz-Sanchez; Etelvina Gandara

2016-01-01

Phylogenetic information provides insight into the ecological and evolutionary processes that organize species assemblages. We compared patterns of phylogenetic diversity among macromycete and woody plant communities along a steep elevational gradient in eastern Mexico to better understand the evolutionary processes that structure their communities. Macrofungi and...

Valleys and Hillslopes: A Geomorphic Foundation for Landscape Ecology

NASA Astrophysics Data System (ADS)

Martin, Y. E.; Johnson, E. A.

2004-12-01

Moisture-nutrient gradients have been found to be the most important environmental gradients determining the distribution and composition of plant communities. Landscapes on which plant communities exist are composed of valleys and ridgelines, with hillslopes in between them. Since water flow paths are directed down slopes, processes determining hillslope morphology and arrangement play an essential role in plant community organization and dynamics. Hillslope morphology, substrate characteristics and climate determine flow routing and water budgets along slopes. Wetness is a function of transmissivity, contributing area and slope gradient. Movement of nutrients along hillslopes generally follows wetness values, and is affected by soil type. Plant species have different tolerances to wetness and nutrients; hillslope length and slope angle determine the moisture-nutrient gradient, and in turn the shape of plant tolerance curves. Temporal scales required for significant topographic change along hillslopes may often be long compared to those for plant community dynamics. When considered in landscape ecology, hillslope shape and arrangement are thus often considered constants. Although landscape morphology may change over time and among different regions (with tectonic, geomorphic and climatic processes leaving their imprints on landscapes), an attempt has been made in the literature to put forth robust topographic scaling relations. This paper, using a series of examples, explores connections between landscape structure and plant communities. For example, Hack's law states that drainage basins become more elongate as area increases. This implies that basins should have approximately the same proportion of landscape in each hillslope position, suggesting some constancy in contributing area patterns for hillslopes in different-sized basins. Distributions of wetness values and plant population tolerance curves seem to confirm this for smaller basins. Hillslope length and steepness are related to drainage density and relative relief. Various studies have sought relations between drainage density and slope gradient; the latter is a determinant of wetness values. Studies have found both negative and positive correlations between drainage density and slope gradient. The nature of hillslope processes (e.g., overland flow vs. mass wasting dominated, or quickly eroding vs. slowly eroding landscapes) has been used to explain the correlation. It has also been suggested that the degree of channelization may be important in determining slope steepness. Plant species respond to steeper slopes by having narrower tolerance curves and less overlap with other species. This has important implications for biodiversity and plant community organization.

Enhanced stochastic fluctuations to measure steep adhesive energy landscapes

PubMed Central

Haider, Ahmad; Potter, Daniel; Sulchek, Todd A.

2016-01-01

Free-energy landscapes govern the behavior of all interactions in the presence of thermal fluctuations in the fields of physical chemistry, materials sciences, and the biological sciences. From the energy landscape, critical information about an interaction, such as the reaction kinetic rates, bond lifetimes, and the presence of intermediate states, can be determined. Despite the importance of energy landscapes to understanding reaction mechanisms, most experiments do not directly measure energy landscapes, particularly for interactions with steep force gradients that lead to premature jump to contact of the probe and insufficient sampling of transition regions. Here we present an atomic force microscopy (AFM) approach for measuring energy landscapes that increases sampling of strongly adhesive interactions by using white-noise excitation to enhance the cantilever’s thermal fluctuations. The enhanced fluctuations enable the recording of subtle deviations from a harmonic potential to accurately reconstruct interfacial energy landscapes with steep gradients. Comparing the measured energy landscape with adhesive force measurements reveals the existence of an optimal excitation voltage that enables the cantilever fluctuations to fully sample the shape and depth of the energy surface. PMID:27911778

Flux tube gyrokinetic simulations of the edge pedestal

NASA Astrophysics Data System (ADS)

Parker, Scott; Wan, Weigang; Chen, Yang

2011-10-01

The linear instabilities of DIII-D H-mode pedestal are studied with gyrokinetic micro-turbulence simulations. The simulation code GEM is an electromagnetic δf code with global tokamak geometry in the form of Miller equilibrium. Local flux tube simulations are carried out for multiple positions of two DIII-D profiles: shot #98889 and shot #131997. Near the top of the pedestal, the instability is clearly ITG. The dominant instability of the pedestal appears at the steep gradient region, and it is identified as a low frequency mode mostly driven by electron temperature gradient. The mode propagates along the electron diamagnetic direction for low n and may propagate along the ion direction for high n. At some positions near the steep gradient region, an ion instability is found which shows some characteristics of kinetic ballooning mode (KBM). These results will be compared to the results of E. Wang et al. and D. Fulton et al. in the same session. We thank R. Groebner and P. Snyder for providing experimental profiles and helpful discussions.

Kinetic simulation of edge instability in fusion plasmas

NASA Astrophysics Data System (ADS)

Fulton, Daniel Patrick

In this work, gyrokinetic simulations in edge plasmas of both tokamaks and field reversed. configurations (FRC) have been carried out using the Gyrokinetic Toroidal Code (GTC) and A New Code (ANC) has been formulated for cross-separatrix FRC simulation. In the tokamak edge, turbulent transport in the pedestal of an H-mode DIII-D plasma is. studied via simulations of electrostatic driftwaves. Annulus geometry is used and simulations focus on two radial locations corresponding to the pedestal top with mild pressure gradient and steep pressure gradient. A reactive trapped electron instability with typical ballooning mode structure is excited in the pedestal top. At the steep gradient, the electrostatic instability exhibits unusual mode structure, peaking at poloidal angles theta=+- pi/2. Simulations find this unusual mode structure is due to steep pressure gradients in the pedestal but not due to the particular DIII-D magnetic geometry. Realistic DIII-D geometry has a stabilizing effect compared to a simple circular tokamak geometry. Driftwave instability in FRC is studied for the first time using gyrokinetic simulation. GTC. is upgraded to treat realistic equilibrium calculated by an MHD equilibrium code. Electrostatic local simulations in outer closed flux surfaces find ion-scale modes are stable due to the large ion gyroradius and that electron drift-interchange modes are excited by electron temperature gradient and bad magnetic curvature. In the scrape-off layer (SOL) ion-scale modes are excited by density gradient and bad curvature. Collisions have weak effects on instabilities both in the core and SOL. Simulation results are consistent with density fluctuation measurements in the C-2 experiment using Doppler backscattering (DBS). The critical density gradients measured by the DBS qualitatively agree with the linear instability threshold calculated by GTC simulations. One outstanding critical issue in the FRC is the interplay between turbulence in the FRC. core and SOL regions. While the magnetic flux coordinates used by GTC provide a number of computational advantages, they present unique challenges at the magnetic field separatrix. To address this limitation, a new code, capable of coupled core-SOL simulations, is formulated, implemented, and successfully verified.

Physiological community ecology: variation in metabolic activity of ecologically important rocky intertidal invertebrates along environmental gradients.

PubMed

Dahlhoff, Elizabeth P; Stillman, Jonathon H; Menge, Bruce A

2002-08-01

Rocky intertidal invertebrates live in heterogeneous habitats characterized by steep gradients in wave activity, tidal flux, temperature, food quality and food availability. These environmental factors impact metabolic activity via changes in energy input and stress-induced alteration of energetic demands. For keystone species, small environmentally induced shifts in metabolic activity may lead to disproportionately large impacts on community structure via changes in growth or survival of these key species. Here we use biochemical indicators to assess how natural differences in wave exposure, temperature and food availability may affect metabolic activity of mussels, barnacles, whelks and sea stars living at rocky intertidal sites with different physical and oceanographic characteristics. We show that oxygen consumption rate is correlated with the activity of key metabolic enzymes (e.g., citrate synthase and malate dehydrogenase) for some intertidal species, and concentrations of these enzymes in certain tissues are lower for starved individuals than for those that are well fed. We also show that the ratio of RNA to DNA (an index of protein synthetic capacity) is highly variable in nature and correlates with short-term changes in food availability. We also observed striking patterns in enzyme activity and RNA/DNA in nature, which are related to differences in rocky intertidal community structure. Differences among species and habitats are most pronounced in summer and are linked to high nearshore productivity at sites favored by suspension feeders and to exposure to stressful low-tide air temperatures in areas of low wave splash. These studies illustrate the great promise of using biochemical indicators to test ecological models, which predict changes in community structure along environmental gradients. Our results also suggest that biochemical indices must be carefully validated with laboratory studies, so that the indicator selected is likely to respond to the environmental variables of interest.

Heterotrophic Proteobacteria in the vicinity of diffuse hydrothermal venting.

PubMed

Meier, Dimitri V; Bach, Wolfgang; Girguis, Peter R; Gruber-Vodicka, Harald R; Reeves, Eoghan P; Richter, Michael; Vidoudez, Charles; Amann, Rudolf; Meyerdierks, Anke

2016-12-01

Deep-sea hydrothermal vents are highly dynamic habitats characterized by steep temperature and chemical gradients. The oxidation of reduced compounds dissolved in the venting fluids fuels primary production providing the basis for extensive life. Until recently studies of microbial vent communities have focused primarily on chemolithoautotrophic organisms. In our study, we targeted the change of microbial community compositions along mixing gradients, focusing on distribution and capabilities of heterotrophic microorganisms. Samples were retrieved from different venting areas within the Menez Gwen hydrothermal field, taken along mixing gradients, including diffuse fluid discharge points, their immediate surroundings and the buoyant parts of hydrothermal plumes. High throughput 16S rRNA gene amplicon sequencing, fluorescence in situ hybridization, and targeted metagenome analysis were combined with geochemical analyses. Close to diffuse venting orifices dominated by chemolithoautotrophic Epsilonproteobacteria, in areas where environmental conditions still supported chemolithoautotrophic processes, we detected microbial communities enriched for versatile heterotrophic Alpha- and Gammaproteobacteria. The potential for alkane degradation could be shown for several genera and yet uncultured clades. We propose that hotspots of chemolithoautotrophic life support a 'belt' of heterotrophic bacteria significantly different from the dominating oligotrophic microbiota of the deep sea. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

Fast, high-resolution 3D dosimetry utilizing a novel optical-CT scanner incorporating tertiary telecentric collimation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sakhalkar, H. S.; Oldham, M.

2008-01-15

This study introduces a charge coupled device (CCD) area detector based optical-computed tomography (optical-CT) scanner for comprehensive verification of radiation dose distributions recorded in nonscattering radiochromic dosimeters. Defining characteristics include: (i) a very fast scanning time of {approx}5 min to acquire a complete three-dimensional (3D) dataset, (ii) improved image formation through the use of custom telecentric optics, which ensures accurate projection images and minimizes artifacts from scattered and stray-light sources, and (iii) high resolution (potentially 50 {mu}m) isotropic 3D dose readout. The performance of the CCD scanner for 3D dose readout was evaluated by comparison with independent 3D readout frommore » the single laser beam OCTOPUS-scanner for the same PRESAGE dosimeters. The OCTOPUS scanner was considered the 'gold standard' technique in light of prior studies demonstrating its accuracy. Additional comparisons were made against calculated dose distributions from the ECLIPSE treatment-planning system. Dose readout for the following treatments were investigated: (i) a single rectangular beam irradiation to investigate small field and very steep dose gradient dosimetry away from edge effects, (ii) a 2-field open beam parallel-opposed irradiation to investigate dosimetry along steep dose gradients, and (iii) a 7-field intensity modulated radiation therapy (IMRT) irradiation to investigate dosimetry for complex treatment delivery involving modulation of fluence and for dosimetry along moderate dose gradients. Dose profiles, dose-difference plots, and gamma maps were employed to evaluate quantitative estimates of agreement between independently measured and calculated dose distributions. Results indicated that dose readout from the CCD scanner was in agreement with independent gold-standard readout from the OCTOPUS-scanner as well as the calculated ECLIPSE dose distribution for all treatments, except in regions within a few millimeters of the edge of the dosimeter, where edge artifact is predominant. Agreement of line profiles was observed, even along steep dose gradients. Dose difference plots indicated that the CCD scanner dose readout differed from the OCTOPUSscanner readout and ECLIPSE calculations by {approx}10% along steep dose gradients and by {approx}5% along moderate dose gradients. Gamma maps (3% dose-difference and 3 mm distance-to-agreement acceptance criteria) revealed agreement, except for regions within 5 mm of the edge of the dosimeter where the edge artifact occurs. In summary, the data demonstrate feasibility of using the fast, high-resolution CCD scanner for comprehensive 3D dosimetry in all applications, except where dose readout is required close to the edges of the dosimeter. Further work is ongoing to reduce this artifact.« less

Highly local environmental variability promotes intrapopulation divergence of quantitative traits: an example from tropical rain forest trees.

PubMed

Brousseau, Louise; Bonal, Damien; Cigna, Jeremy; Scotti, Ivan

2013-10-01

In habitat mosaics, plant populations face environmental heterogeneity over short geographical distances. Such steep environmental gradients can induce ecological divergence. Lowland rainforests of the Guiana Shield are characterized by sharp, short-distance environmental variations related to topography and soil characteristics (from waterlogged bottomlands on hydromorphic soils to well-drained terra firme on ferralitic soils). Continuous plant populations distributed along such gradients are an interesting system to study intrapopulation divergence at highly local scales. This study tested (1) whether conspecific populations growing in different habitats diverge at functional traits, and (2) whether they diverge in the same way as congeneric species having different habitat preferences. Phenotypic differentiation was studied within continuous populations occupying different habitats for two congeneric, sympatric, and ecologically divergent tree species (Eperua falcata and E. grandiflora, Fabaceae). Over 3000 seeds collected from three habitats were germinated and grown in a common garden experiment, and 23 morphological, biomass, resource allocation and physiological traits were measured. In both species, seedling populations native of different habitats displayed phenotypic divergence for several traits (including seedling growth, biomass allocation, leaf chemistry, photosynthesis and carbon isotope composition). This may occur through heritable genetic variation or other maternally inherited effects. For a sub-set of traits, the intraspecific divergence associated with environmental variation coincided with interspecific divergence. The results indicate that mother trees from different habitats transmit divergent trait values to their progeny, and suggest that local environmental variation selects for different trait optima even at a very local spatial scale. Traits for which differentiation within species follows the same pattern as differentiation between species indicate that the same ecological processes underlie intra- and interspecific variation.

Highly local environmental variability promotes intrapopulation divergence of quantitative traits: an example from tropical rain forest trees

PubMed Central

Brousseau, Louise; Bonal, Damien; Cigna, Jeremy; Scotti, Ivan

2013-01-01

Background and Aims In habitat mosaics, plant populations face environmental heterogeneity over short geographical distances. Such steep environmental gradients can induce ecological divergence. Lowland rainforests of the Guiana Shield are characterized by sharp, short-distance environmental variations related to topography and soil characteristics (from waterlogged bottomlands on hydromorphic soils to well-drained terra firme on ferralitic soils). Continuous plant populations distributed along such gradients are an interesting system to study intrapopulation divergence at highly local scales. This study tested (1) whether conspecific populations growing in different habitats diverge at functional traits, and (2) whether they diverge in the same way as congeneric species having different habitat preferences. Methods Phenotypic differentiation was studied within continuous populations occupying different habitats for two congeneric, sympatric, and ecologically divergent tree species (Eperua falcata and E. grandiflora, Fabaceae). Over 3000 seeds collected from three habitats were germinated and grown in a common garden experiment, and 23 morphological, biomass, resource allocation and physiological traits were measured. Key Results In both species, seedling populations native of different habitats displayed phenotypic divergence for several traits (including seedling growth, biomass allocation, leaf chemistry, photosynthesis and carbon isotope composition). This may occur through heritable genetic variation or other maternally inherited effects. For a sub-set of traits, the intraspecific divergence associated with environmental variation coincided with interspecific divergence. Conclusions The results indicate that mother trees from different habitats transmit divergent trait values to their progeny, and suggest that local environmental variation selects for different trait optima even at a very local spatial scale. Traits for which differentiation within species follows the same pattern as differentiation between species indicate that the same ecological processes underlie intra- and interspecific variation. PMID:24023042

Forest reproduction along a climatic gradient in the Sierra Nevada, California

USGS Publications Warehouse

van Mantgem, Phillip J.; Stephenson, Nathan L.; Keeley, Jon E.

2006-01-01

To elucidate broad-scale environmental controls of coniferous forest reproduction in the Sierra Nevada, California, we monitored reproduction for 5 years in 47 plots arrayed across a steep elevational (climatic) gradient. We found that both absolute seedling densities (stems < 1.37 m) and seedling densities relative to overstory parent tree basal area declined sharply with elevation. Rates of seedling turnover (the average of birth and death rates) also declined with elevation. In contrast, seed production was not predicted by elevation and was highly variable from year to year. During a mast year of seed production, the intensity of masting was uneven among plots. Seedling densities were elevated only during the single year immediately following the mast year, suggesting reproduction in our forests may be primarily limited by abiotic factors such as the availability of suitable sites and weather. Disturbance also clearly affected reproduction; plots that had recently burned had significantly higher seedling to parent tree ratios for Abies species, suggesting that even though established Abies concolor may be relatively susceptible to fire, the species can recover rapidly through prolific reproduction. Since reproductive failures may be our earliest signal of changing forest conditions, seedling dynamics could provide a sensitive, if variable, indicator of environmental changes.

Ultrasensitivity by Molecular Titration in Spatially Propagating Enzymatic Reactions

PubMed Central

Semenov, Sergey N.; Markvoort, Albert J.; Gevers, Wouter B.L.; Piruska, Aigars; de Greef, Tom F.A.; Huck, Wilhelm T.S.

2013-01-01

Delineating design principles of biological systems by reconstitution of purified components offers a platform to gauge the influence of critical physicochemical parameters on minimal biological systems of reduced complexity. Here we unravel the effect of strong reversible inhibitors on the spatiotemporal propagation of enzymatic reactions in a confined environment in vitro. We use micropatterned, enzyme-laden agarose gels which are stamped on polyacrylamide films containing immobilized substrates and reversible inhibitors. Quantitative fluorescence imaging combined with detailed numerical simulations of the reaction-diffusion process reveal that a shallow gradient of enzyme is converted into a steep product gradient by addition of strong inhibitors, consistent with a mathematical model of molecular titration. The results confirm that ultrasensitive and threshold effects at the molecular level can convert a graded input signal to a steep spatial response at macroscopic length scales. PMID:23972857

The role of shelf morphology and antecedent setting in the preservation of palaeo-shoreline (beachrock and aeolianite) sequences: the SE African shelf

NASA Astrophysics Data System (ADS)

Green, Andrew N.; Cooper, J. Andrew G.; Salzmann, Leslee

2018-02-01

On the SE African shelf, a submerged shoreline at a depth of 60 m is examined and its attributes compared between two shelf sectors with different morphologies, yet similar energy regimes. The aim is to assess the controls of antecedent conditioning on shoreline development and later preservation from transgressive ravinement. Using a combination of multibeam bathymetry and single-channel seismic profiles, the stratigraphy and morphology of the shoreline is investigated. Low-gradient bedrock examples reveal several distinctive seismic facies, including onlapping chaotic reflector packages which are interpreted as calcarenite rubble fields. These palaeo-shorelines possess planform equilibrium morphologies, including parabolic dunes and blowout forms along with relict shore platforms. They are strongly associated with incised valleys of last glacial maximum age which underlie the shoreline locations; these provide wide, back -barrier accommodation space during transgression. In contrast, palaeo-shorelines on the steeper-gradient shelf have a simpler stratigraphic arrangement. They are not as well preserved, are generally covered by thick drapes of sediment, and lack the elaborate planform morphologies of their lower-shelf gradient equivalents. Isolated incised valleys and the steep bedrock gradient limit accommodation space. The comparison indicates that antecedent bedrock slope and available accommodation are amongst the dominant controls on overstepping, and thus potential preservation, of palaeo-shorelines on the shelf. Lower-gradient shelves not only promote rapid shoreline translation but, together with wide, sandy back -barrier accommodation, also foster larger barrier volumes. In suitable climates such as in the Mediterranean and other sub-tropical areas, the ensuing shoreline stability promotes rapid and effective cementation of the barrier. In comparison, steep bedrock profiles with limited back -barrier accommodation have much lower preservation potential. Transgressive ravinement is more focussed on steep slopes, effectively removing more material during the ravinement process. The more dynamic environment may also reduce the effectiveness of diagenesis. The potential of beachrock and aeolianite palaeo-shorelines as submerged sea-level indicators may be optimal in low-gradient settings in Mediterranean to subtropical environments.

`Surface-Layer' momentum fluxes in nocturnal slope flows over steep terrain

NASA Astrophysics Data System (ADS)

Oldroyd, H. J.; Pardyjak, E.; Higgins, C. W.; Parlange, M. B.

2017-12-01

A common working definition for the `surface layer' is the lowest 10% of the atmospheric boundary layer (ABL) where the turbulent fluxes are essentially constant. The latter part of this definition is a critical assumption that must hold for accurate flux estimations from land-surface models, wall models, similarity theory, flux-gradient relations and bulk transfer methods. We present cases from observed momentum fluxes in nocturnal slope flows over steep (35.5 degree), alpine terrain in Val Ferret, Switzerland that satisfy the classical definitions of the surface layer and other cases where no traditional surface layer is observed. These cases broadly fall into two distinct flow regimes occurring under clear-sky conditions: (1) buoyancy-driven, `katabatic flow', characterized by an elevated velocity maximum (katabatic jet peak) and (2) `downslope winds', for which larger-scale forcing prevents formation of a katabatic jet. Velocity profiles in downslope wind cases are quite similar to logarithmic profiles typically observed over horizontal and homogeneous terrain, and the corresponding momentum fluxes roughly resemble a constant-flux surface-layer. Contrastingly, velocity profiles in the katabatic regime exhibit a jet-like shape. This jet strongly modulates the corresponding momentum fluxes, which exhibit strong gradients over the shallow katabatic layer and usually change sign near the jet peak, where the velocity gradients also change sign. However, a counter-gradient momentum flux is frequently observed near the jet peak (and sometimes at higher levels), suggesting strong non-local turbulent transport within the katabatic jet layer. We compare our observations with katabatic flow theories and observational studies over shallow-angle slopes and use co-spectral analyses to better identify and understand the non-local transport dynamics. Finally, we show that because of the counter-gradient momentum fluxes, surface layer stability and even local stability can be difficult to characterize because the counter-gradient momentum flux represents a sink in the shear term of turbulence kinetic energy budget equation. These results have broad implications for stability-based modeling and general definitions and assumptions used for the ABL and so-called `surface layer' over steep terrain.

Physiological Limits along an Elevational Gradient in a Radiation of Montane Ground Beetles

PubMed Central

Slatyer, Rachel A.; Schoville, Sean D.

2016-01-01

A central challenge in ecology and biogeography is to determine the extent to which physiological constraints govern the geographic ranges of species along environmental gradients. This study tests the hypothesis that temperature and desiccation tolerance are associated with the elevational ranges of 12 ground beetle species (genus Nebria) occurring on Mt. Rainier, Washington, U.S.A. Species from higher elevations did not have greater cold tolerance limits than lower-elevation species (all species ranged from -3.5 to -4.1°C), despite a steep decline in minimum temperature with elevation. Although heat tolerance limits varied among species (from 32.0 to 37.0°C), this variation was not generally associated with the relative elevational range of a species. Temperature gradients and acute thermal tolerance do not support the hypothesis that physiological constraints drive species turnover with elevation. Measurements of intraspecific variation in thermal tolerance limits were not significant for individuals taken at different elevations on Mt. Rainier, or from other mountains in Washington and Oregon. Desiccation resistance was also not associated with a species’ elevational distribution. Our combined results contrast with previously-detected latitudinal gradients in acute physiological limits among insects and suggest that other processes such as chronic thermal stress or biotic interactions might be more important in constraining elevational distributions in this system. PMID:27043311

A Diffusion Approximation Based on Renewal Processes with Applications to Strongly Biased Run-Tumble Motion.

PubMed

Thygesen, Uffe Høgsbro

2016-03-01

We consider organisms which use a renewal strategy such as run-tumble when moving in space, for example to perform chemotaxis in chemical gradients. We derive a diffusion approximation for the motion, applying a central limit theorem due to Anscombe for renewal-reward processes; this theorem has not previously been applied in this context. Our results extend previous work, which has established the mean drift but not the diffusivity. For a classical model of tumble rates applied to chemotaxis, we find that the resulting chemotactic drift saturates to the swimming velocity of the organism when the chemical gradients grow increasingly steep. The dispersal becomes anisotropic in steep gradients, with larger dispersal across the gradient than along the gradient. In contrast to one-dimensional settings, strong bias increases dispersal. We next include Brownian rotation in the model and find that, in limit of high chemotactic sensitivity, the chemotactic drift is 64% of the swimming velocity, independent of the magnitude of the Brownian rotation. We finally derive characteristic timescales of the motion that can be used to assess whether the diffusion limit is justified in a given situation. The proposed technique for obtaining diffusion approximations is conceptually and computationally simple, and applicable also when statistics of the motion is obtained empirically or through Monte Carlo simulation of the motion.

Trade-Offs between Growth Rate, Tree Size and Lifespan of Mountain Pine (Pinus montana) in the Swiss National Park

PubMed Central

Bigler, Christof

2016-01-01

A within-species trade-off between growth rates and lifespan has been observed across different taxa of trees, however, there is some uncertainty whether this trade-off also applies to shade-intolerant tree species. The main objective of this study was to investigate the relationships between radial growth, tree size and lifespan of shade-intolerant mountain pines. For 200 dead standing mountain pines (Pinus montana) located along gradients of aspect, slope steepness and elevation in the Swiss National Park, radial annual growth rates and lifespan were reconstructed. While early growth (i.e. mean tree-ring width over the first 50 years) correlated positively with diameter at the time of tree death, a negative correlation resulted with lifespan, i.e. rapidly growing mountain pines face a trade-off between reaching a large diameter at the cost of early tree death. Slowly growing mountain pines may reach a large diameter and a long lifespan, but risk to die young at a small size. Early growth was not correlated with temperature or precipitation over the growing period. Variability in lifespan was further contingent on aspect, slope steepness and elevation. The shade-intolerant mountain pines follow diverging growth trajectories that are imposed by extrinsic environmental influences. The resulting trade-offs between growth rate, tree size and lifespan advance our understanding of tree population dynamics, which may ultimately improve projections of forest dynamics under changing environmental conditions. PMID:26930294

Non-Native Plant Invasion along Elevation and Canopy Closure Gradients in a Middle Rocky Mountain Ecosystem

PubMed Central

Averett, Joshua P.; McCune, Bruce; Parks, Catherine G.; Naylor, Bridgett J.; DelCurto, Tim; Mata-González, Ricardo

2016-01-01

Mountain environments are currently among the ecosystems least invaded by non-native species; however, mountains are increasingly under threat of non-native plant invasion. The slow pace of exotic plant invasions in mountain ecosystems is likely due to a combination of low anthropogenic disturbances, low propagule supply, and extreme/steep environmental gradients. The importance of any one of these factors is debated and likely ecosystem dependent. We evaluated the importance of various correlates of plant invasions in the Wallowa Mountain Range of northeastern Oregon and explored whether non-native species distributions differed from native species along an elevation gradient. Vascular plant communities were sampled in summer 2012 along three mountain roads. Transects (n = 20) were evenly stratified by elevation (~70 m intervals) along each road. Vascular plant species abundances and environmental parameters were measured. We used indicator species analysis to identify habitat affinities for non-native species. Plots were ordinated in species space, joint plots and non-parametric multiplicative regression were used to relate species and community variation to environmental variables. Non-native species richness decreased continuously with increasing elevation. In contrast, native species richness displayed a unimodal distribution with maximum richness occurring at mid–elevations. Species composition was strongly related to elevation and canopy openness. Overlays of trait and environmental factors onto non-metric multidimensional ordinations identified the montane-subalpine community transition and over-story canopy closure exceeding 60% as potential barriers to non-native species establishment. Unlike native species, non-native species showed little evidence for high-elevation or closed-canopy specialization. These data suggest that non-native plants currently found in the Wallowa Mountains are dependent on open canopies and disturbance for establishment in low and mid elevations. Current management objectives including restoration to more open canopies in dry Rocky Mountain forests, may increase immigration pressure of non-native plants from lower elevations into the montane and subalpine zones. PMID:26824750

Non-Native Plant Invasion along Elevation and Canopy Closure Gradients in a Middle Rocky Mountain Ecosystem.

PubMed

Averett, Joshua P; McCune, Bruce; Parks, Catherine G; Naylor, Bridgett J; DelCurto, Tim; Mata-González, Ricardo

2016-01-01

Mountain environments are currently among the ecosystems least invaded by non-native species; however, mountains are increasingly under threat of non-native plant invasion. The slow pace of exotic plant invasions in mountain ecosystems is likely due to a combination of low anthropogenic disturbances, low propagule supply, and extreme/steep environmental gradients. The importance of any one of these factors is debated and likely ecosystem dependent. We evaluated the importance of various correlates of plant invasions in the Wallowa Mountain Range of northeastern Oregon and explored whether non-native species distributions differed from native species along an elevation gradient. Vascular plant communities were sampled in summer 2012 along three mountain roads. Transects (n = 20) were evenly stratified by elevation (~70 m intervals) along each road. Vascular plant species abundances and environmental parameters were measured. We used indicator species analysis to identify habitat affinities for non-native species. Plots were ordinated in species space, joint plots and non-parametric multiplicative regression were used to relate species and community variation to environmental variables. Non-native species richness decreased continuously with increasing elevation. In contrast, native species richness displayed a unimodal distribution with maximum richness occurring at mid-elevations. Species composition was strongly related to elevation and canopy openness. Overlays of trait and environmental factors onto non-metric multidimensional ordinations identified the montane-subalpine community transition and over-story canopy closure exceeding 60% as potential barriers to non-native species establishment. Unlike native species, non-native species showed little evidence for high-elevation or closed-canopy specialization. These data suggest that non-native plants currently found in the Wallowa Mountains are dependent on open canopies and disturbance for establishment in low and mid elevations. Current management objectives including restoration to more open canopies in dry Rocky Mountain forests, may increase immigration pressure of non-native plants from lower elevations into the montane and subalpine zones.

Hardwood silviculture and skyline yarding on steep slopes: economic and environmental impacts

Treesearch

John E. Baumgras; Chris B. LeDoux

1995-01-01

Ameliorating the visual and environmental impact associated with harvesting hardwoods on steep slopes will require the efficient use of skyline yarding along with silvicultural alternatives to clearcutting. In evaluating the effects of these alternatives on harvesting revenue, results of field studies and computer simulations were used to estimate costs and revenue for...

Are income-related differences in active travel associated with physical environmental characteristics? A multi-level ecological approach.

PubMed

Rind, Esther; Shortt, Niamh; Mitchell, Richard; Richardson, Elizabeth A; Pearce, Jamie

2015-06-05

Rates of active travel vary by socio-economic position, with higher rates generally observed among less affluent populations. Aspects of both social and built environments have been shown to affect active travel, but little research has explored the influence of physical environmental characteristics, and less has examined whether physical environment affects socio-economic inequality in active travel. This study explored income-related differences in active travel in relation to multiple physical environmental characteristics including air pollution, climate and levels of green space, in urban areas across England. We hypothesised that any gradient in the relationship between income and active travel would be least pronounced in the least physically environmentally-deprived areas where higher income populations may be more likely to choose active transport as a means of travel. Adults aged 16+ living in urban areas (n = 20,146) were selected from the 2002 and 2003 waves of the UK National Travel Survey. The mode of all short non-recreational trips undertaken by the sample was identified (n = 205,673). Three-level binary logistic regression models were used to explore how associations between the trip being active (by bike/walking) and three income groups, varied by level of multiple physical environmental deprivation. Likelihood of making an active trip among the lowest income group appeared unaffected by physical environmental deprivation; 15.4% of their non-recreational trips were active in both the least and most environmentally-deprived areas. The income-related gradient in making active trips remained steep in the least environmentally-deprived areas because those in the highest income groups were markedly less likely to choose active travel when physical environment was 'good', compared to those on the lowest incomes (OR = 0.44, 95% CI = 0.22 to 0.89). The socio-economic gradient in active travel seems independent of physical environmental characteristics. Whilst more affluent populations enjoy advantages on some health outcomes, they will still benefit from increasing their levels of physical activity through active travel. Benefits of active travel to the whole community would include reduced vehicle emissions, reduced carbon consumption, the preservation or enhancement of infrastructure and the presentation of a 'normalised' behaviour.

Heterogeneous Landscapes on Steep Slopes at Low Altitudes as Hotspots of Bird Diversity in a Hilly Region of Nepal in the Central Himalayas.

PubMed

Basnet, Tej B; Rokaya, Maan B; Bhattarai, Bishnu P; Münzbergová, Zuzana

2016-01-01

Understanding factors determining the distribution of species is a key requirement for protecting diversity in a specific area. The aim of this study was to explore the factors affecting diversity and distribution of species of birds on different forested hills in central Nepal. The area is rich in species of birds. Because the area is characterized by steep gradients, we were also interested in the importance of altitude in determining the diversity and species composition of the bird communities. We assessed bird diversity and species composition based on point observations along a gradient of increasing altitude in two valleys (Kathmandu and Palung) in central Nepal. Data on environmental variables were also collected in order to identify the main determinants of bird diversity and species composition of the bird communities. We recorded 6522 individual birds belonging to 146 species, 77 genera and 23 families. Resident birds made up 80% (117 species) of the total dataset. The study supported the original expectation that altitude is a major determinant of species richness and composition of bird communities in the area. More diverse bird communities were found also in areas with steeper slopes. This together with the positive effect of greater heterogeneity suggests that forests on steep slopes intermixed with patches of open habitats on shallow soil at large spatial scales are more important for diverse bird communities than more disturbed habitats on shallow slopes. In addition, we demonstrated that while different habitat characteristics such as presence of forests edges and shrubs play an important role in driving species composition, but they do not affect species richness. This indicates that while habitat conditions are important determinants of the distribution of specific species, the number of niches is determined by large scale characteristics, such as landscape level habitat heterogeneity and altitude. Thus, to protect bird diversity in the mid-hills of central Nepal, we should maintain diverse local habitats (viz. forest, shrubs, open land, etc.) but also make sure the natural habitats on steeper slopes with large scale heterogeneity are maintained.

Heterogeneous Landscapes on Steep Slopes at Low Altitudes as Hotspots of Bird Diversity in a Hilly Region of Nepal in the Central Himalayas

PubMed Central

Basnet, Tej B.; Rokaya, Maan B.; Bhattarai, Bishnu P.; Münzbergová, Zuzana

2016-01-01

Understanding factors determining the distribution of species is a key requirement for protecting diversity in a specific area. The aim of this study was to explore the factors affecting diversity and distribution of species of birds on different forested hills in central Nepal. The area is rich in species of birds. Because the area is characterized by steep gradients, we were also interested in the importance of altitude in determining the diversity and species composition of the bird communities. We assessed bird diversity and species composition based on point observations along a gradient of increasing altitude in two valleys (Kathmandu and Palung) in central Nepal. Data on environmental variables were also collected in order to identify the main determinants of bird diversity and species composition of the bird communities. We recorded 6522 individual birds belonging to 146 species, 77 genera and 23 families. Resident birds made up 80% (117 species) of the total dataset. The study supported the original expectation that altitude is a major determinant of species richness and composition of bird communities in the area. More diverse bird communities were found also in areas with steeper slopes. This together with the positive effect of greater heterogeneity suggests that forests on steep slopes intermixed with patches of open habitats on shallow soil at large spatial scales are more important for diverse bird communities than more disturbed habitats on shallow slopes. In addition, we demonstrated that while different habitat characteristics such as presence of forests edges and shrubs play an important role in driving species composition, but they do not affect species richness. This indicates that while habitat conditions are important determinants of the distribution of specific species, the number of niches is determined by large scale characteristics, such as landscape level habitat heterogeneity and altitude. Thus, to protect bird diversity in the mid-hills of central Nepal, we should maintain diverse local habitats (viz. forest, shrubs, open land, etc.) but also make sure the natural habitats on steeper slopes with large scale heterogeneity are maintained. PMID:26938616

The urban environmental gradient: Anthropogenic influences on the spatial and temporal distributions of lead and zinc in sediments

USGS Publications Warehouse

Callender, Edward; Rice, Karen C.

2000-01-01

Urban settings are a focal point for environmental contamination due to emissions from industrial and municipal activities and the widespread use of motor vehicles. As part of the National Water-Quality Assessment Program of the U.S. Geological Survey, streambed-sediment and dated reservoir-sediment samples were collected from the Chattahoochee River Basin and analyzed for total lead (Pb) and zinc (Zn) concentrations. The sampling transect extends from northern Georgia, through Atlanta, to the Gulf of Mexico and reflects a steep gradient in population density from nearly 1000 people/km2 in the Atlanta Metropolitan Area to fewer than 50 people/km2 in rural areas of southern Georgia and northern Florida. Correlations among population density, traffic density, and total and anthropogenic Pb and Zn concentrations indicate that population density is strongly related to traffic density and is a predictor of Pb and Zn concentrations in the environment derived from anthropogenic activities. Differences in the distributions of total Pb and Zn concentrations along the urban−suburban−rural gradient from Atlanta to the Florida Panhandle are related to temporal and spatial processes. That is, with the removal of leaded gasoline starting in the late 1970s, peak Pb concentrations have decreased to the present. Conversely, increased vehicular usage has kept Zn concentrations elevated in runoff from population centers, which is reflected in the continued enrichment of Zn in aquatic sediments. Sediments from rural areas also contain elevated concentrations of Zn, possibly in response to substantial power plant emissions for the region, as well as vehicular traffic.

Exploring the limits of the ``SNB'' multi-group diffusion nonlocal model

NASA Astrophysics Data System (ADS)

Brodrick, Jonathan; Ridgers, Christopher; Kingham, Robert

2014-10-01

A correct treatment of nonlocal transport in the presence of steep temperature gradients found in laser and inertial fusion plasmas has long been highly desirable over the use of an ad-hoc flux limiter. Therefore, an implementation of the ``SNB'' nonlocal model (G P Schurtz, P D Nicolaï & M Busquet, Phys. Plas. 7, 4238 (2000)) has been benchmarked against a fully-implicit kinetic code: IMPACT. A variety of scenarios, including relaxation of temperature sinusoids and Gaussians in addition to continuous laser heating have been investigated. Results highlight the effect of neglecting electron inertia (∂f1/∂ t) as well as question the feasibility of a nonlocal model that does not continuously track the evolution of the distribution function. Deviations from the Spitzer electric fields used in the model across steep gradients are also investigated. Regimes of validity for such a model are identified and discussed, and possible improvements to the model are suggested.

The Growth of the Disk Galaxy UGC8802

NASA Astrophysics Data System (ADS)

Chang, R. X.; Shen, S. Y.; Hou, J. L.

2012-07-01

The disk galaxy UGC8802 has high neutral gas content and a flat profile of star formation rate compared to other disk galaxies with similar stellar mass. It also shows a steep metallicity gradient. We construct a chemical evolution model to explore its growth history by assuming its disk grows gradually from continuous gas infall, which is shaped by a free parameter—the infall-peak time. By adopting the recently observed molecular surface density related star formation law, we show that a late infall-peak time can naturally explain the observed high neutral gas content, while an inside-out disk formation scenario can fairly reproduce the steep oxygen abundance gradient. Our results show that most of the observed features of UGC8802 can be well reproduced by simply "turning the knob" on gas inflow with one single parameter, which implies that the observed properties of gas-rich galaxies could also be modeled in a similar way.

Socioeconomic hierarchy and health gradient in Europe: the role of income inequality and of social origins.

PubMed

Chauvel, Louis; Leist, Anja K

2015-11-14

Health inequalities reflect multidimensional inequality (income, education, and other indicators of socioeconomic position) and vary across countries and welfare regimes. To which extent there is intergenerational transmission of health via parental socioeconomic status has rarely been investigated in comparative perspective. The study sought to explore if different measures of stratification produce the same health gradient and to which extent health gradients of income and of social origins vary with level of living and income inequality. A total of 299,770 observations were available from 18 countries assessed in EU-SILC 2005 and 2011 data, which contain information on social origins. Income inequality (Gini) and level of living were calculated from EU-SILC. Logit rank transformation provided normalized inequalities and distributions of income and social origins up to the extremes of the distribution and was used to investigate net comparable health gradients in detail. Multilevel random-slope models were run to post-estimate best linear unbiased predictors (BLUPs) and related standard deviations of residual intercepts (median health) and slopes (income-health gradients) per country and survey year. Health gradients varied across different measures of stratification, with origins and income producing significant slopes after controls. Income inequality was associated with worse average health, but income inequality and steepness of the health gradient were only marginally associated. Linear health gradients suggest gains in health per rank of income and of origins even at the very extremes of the distribution. Intergenerational transmission of status gains in importance in countries with higher income inequality. Countries differ in the association of income inequality and income-related health gradient, and low income inequality may mask health problems of vulnerable individuals with low status. Not only income inequality, but other country characteristics such as familial orientation play a considerable role in explaining steepness of the health gradient.

Marine Microbial Mats and the Search for Evidence of Life in Deep Time and Space

NASA Technical Reports Server (NTRS)

Des Marais, David J.

2011-01-01

Cyanobacterial mats in extensive seawater evaporation ponds at Guerrero Negro, Baja California, Mexico, have been excellent subjects for microbial ecology research. The studies reviewed here have documented the steep and rapidly changing environmental gradients experienced by mat microorganisms and the very high rates of biogeochemical processes that they maintained. Recent genetic studies have revealed an enormous diversity of bacteria as well as the spatial distribution of Bacteria, Archaea and Eukarya. These findings, together with emerging insights into the intimate interactions between these diverse populations, have contributed substantially to our understanding of the origins, environmental impacts, and biosignatures of photosynthetic microbial mats. The biosignatures (preservable cells, sedimentary fabrics, organic compounds, minerals, stable isotope patterns, etc.) potentially can serve as indicators of past life on early Earth. They also can inform our search for evidence of any life on Mars. Mars exploration has revealed evidence of evaporite deposits and thermal spring deposits; similar deposits on Earth once hosted ancient microbial mat ecosystems.

Stereo transparency and the disparity gradient limit

NASA Technical Reports Server (NTRS)

McKee, Suzanne P.; Verghese, Preeti

2002-01-01

Several studies (Vision Research 15 (1975) 583; Perception 9 (1980) 671) have shown that binocular fusion is limited by the disparity gradient (disparity/distance) separating image points, rather than by their absolute disparity values. Points separated by a gradient >1 appear diplopic. These results are sometimes interpreted as a constraint on human stereo matching, rather than a constraint on fusion. Here we have used psychophysical measurements on stereo transparency to show that human stereo matching is not constrained by a gradient of 1. We created transparent surfaces composed of many pairs of dots, in which each member of a pair was assigned a disparity equal and opposite to the disparity of the other member. For example, each pair could be composed of one dot with a crossed disparity of 6' and the other with uncrossed disparity of 6', vertically separated by a parametrically varied distance. When the vertical separation between the paired dots was small, the disparity gradient for each pair was very steep. Nevertheless, these opponent-disparity dot pairs produced a striking appearance of two transparent surfaces for disparity gradients ranging between 0.5 and 3. The apparent depth separating the two transparent planes was correctly matched to an equivalent disparity defined by two opaque surfaces. A test target presented between the two transparent planes was easily detected, indicating robust segregation of the disparities associated with the paired dots into two transparent surfaces with few mismatches in the target plane. Our simulations using the Tsai-Victor model show that the response profiles produced by scaled disparity-energy mechanisms can account for many of our results on the transparency generated by steep gradients.

On the metallicity gradients of the Galactic disk as revealed by LSS-GAC red clump stars

NASA Astrophysics Data System (ADS)

Huang, Yang; Liu, Xiao-Wei; Zhang, Hua-Wei; Yuan, Hai-Bo; Xiang, Mao-Sheng; Chen, Bing-Qiu; Ren, Juan-Juan; Sun, Ning-Chen; Wang, Chun; Zhang, Yong; Hou, Yong-Hui; Wang, Yue-Fei; Yang, Ming

2015-08-01

Using a sample of over 70 000 red clump (RC) stars with 5%-10% distance accuracy selected from the LAMOST Spectroscopic Survey of the Galactic Anti-center (LSS-GAC), we study the radial and vertical gradients of the Galactic disk(s) mainly in the anti-center direction, covering a significant volume of the disk in the range of projected Galactocentric radius 7 ≤ RGC ≤ 14 kpc and height from the Galactic midplane 0 ≤ |Z| ≤ 3 kpc. Our analysis shows that both the radial and vertical metallicity gradients are negative across much of the volume of the disk that is probed, and they exhibit significant spatial variations. Near the solar circle (7 ≤ RGC ≤ 115 kpc), the radial gradient has a moderately steep, negative slope of -0.08 dex kpc-1 near the midplane (|Z| < 0.1 kpc), and the slope flattens with increasing |Z|. In the outer disk (11.5 < RGC ≤ 14 kpc), the radial gradients have an essentially constant, much less steep slope of -0.01 dex kpc-1 at all heights above the plane, suggesting that the outer disk may have experienced an evolutionary path different from that of the inner disk. The vertical gradients are found to flatten largely with increasing RGC. However, the vertical gradient of the lower disk (0 ≤ |Z| ≤ 1 kpc) is found to flatten with RGC quicker than that of the upper disk (1 < |Z| ≤ 3 kpc). Our results should provide strong constraints on the theory of disk formation and evolution, as well as the underlying physical processes that shape the disk (e.g. gas flows, radial migration, and internal and external perturbations).

Microbial community phylogenetic and trait diversity declines with depth in a marine oxygen minimum zone.

PubMed

Bryant, Jessica A; Stewart, Frank J; Eppley, John M; DeLong, Edward F

2012-07-01

Oxygen minimum zones (OMZs) are natural physical features of the world's oceans. They create steep physiochemical gradients in the water column, which most notably include a dramatic draw down in oxygen concentrations over small vertical distances (<100 m). Microbial communities within OMZs play central roles in ocean and global biogeochemical cycles, yet we still lack a fundamental understanding of how microbial biodiversity is distributed across OMZs. Here, we used metagenomic sequencing to investigate microbial diversity across a vertical gradient in the water column during three seasons in the Eastern Tropical South Pacific (ETSP) OMZ. Based on analysis of small subunit ribosomal RNA (SSU rRNA) gene fragments, we found that both taxonomic and phylogenetic diversity declined steeply along the transition from oxygen-rich surface water to the permanent OMZ. We observed similar declines in the diversity of protein-coding gene categories, suggesting a decrease in functional (trait) diversity with depth. Metrics of functional and trait dispersion indicated that microbial communities are phylogenetically and functionally more overdispersed in oxic waters, but clustered within the OMZ. These dispersion patterns suggest that community assembly drivers (e.g., competition, environmental filtering) vary strikingly across the oxygen gradient. To understand the generality of our findings, we compared OMZ results to two marine depth gradients in subtropical oligotrophic sites and found that the oligotrophic sites did not display similar patterns, likely reflecting unique features found in the OMZ. Finally, we discuss how our results may relate to niche theory, diversity-energy relationships and stress gradients.

Neutral recycling effects on ITG turbulence

DOE PAGES

Stotler, D. P.; Lang, J.; Chang, C. S.; ...

2017-07-04

Here, the effects of recycled neutral atoms on tokamak ion temperature gradient (ITG) driven turbulence have been investigated in a steep edge pedestal, magnetic separatrix configuration, with the full-f edge gryokinetic code XGC1. An adiabatic electron model has been used; hence, the impacts of neutral particles and turbulence on the density gradient are not considered, nor are electromagnetic turbulence effects. The neutral atoms enhance the ITG turbulence, first, by increasing the ion temperature gradient in the pedestal via the cooling effects of charge exchange and, second, by a relative reduction in themore » $$E\\times B$$ shearing rate.« less

Neutral recycling effects on ITG turbulence

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stotler, D. P.; Lang, J.; Chang, C. S.

Here, the effects of recycled neutral atoms on tokamak ion temperature gradient (ITG) driven turbulence have been investigated in a steep edge pedestal, magnetic separatrix configuration, with the full-f edge gryokinetic code XGC1. An adiabatic electron model has been used; hence, the impacts of neutral particles and turbulence on the density gradient are not considered, nor are electromagnetic turbulence effects. The neutral atoms enhance the ITG turbulence, first, by increasing the ion temperature gradient in the pedestal via the cooling effects of charge exchange and, second, by a relative reduction in themore » $$E\\times B$$ shearing rate.« less

Why Are Indian Children So Short? The Role of Birth Order and Son Preference.

PubMed

Jayachandran, Seema; Pandi, Rohini

2017-09-01

Child stunting in India exceeds that in poorer regions like sub-Saharan Africa. Data on over 168,000 children show that, relative to Africa, India's height disadvantage increases sharply with birth order. We posit that India’s steep birth order gradient is due to favoritism toward eldest sons, which affects parents' fertility decisions and resource allocation across children. We show that, within India, the gradient is steeper for high-son-preference regions and religions. The gradient also varies with sibling gender as predicted. A back-of-the-envelope calculation suggests that India's steeper birth order gradient can explain over one-half of the India-Africa gap in average child height.

Climate change and the northern Russian treeline zone.

PubMed

MacDonald, G M; Kremenetski, K V; Beilman, D W

2008-07-12

The Russian treeline is a dynamic ecotone typified by steep gradients in summer temperature and regionally variable gradients in albedo and heat flux. The location of the treeline is largely controlled by summer temperatures and growing season length. Temperatures have responded strongly to twentieth-century global warming and will display a magnified response to future warming. Dendroecological studies indicate enhanced conifer recruitment during the twentieth century. However, conifers have not yet recolonized many areas where trees were present during the Medieval Warm period (ca AD 800-1,300) or the Holocene Thermal Maximum (HTM; ca 10,000-3,000 years ago). Reconstruction of tree distributions during the HTM suggests that the future position of the treeline due to global warming may approximate its former Holocene maximum position. An increased dominance of evergreen tree species in the northern Siberian forests may be an important difference between past and future conditions. Based on the slow rates of treeline expansion observed during the twentieth century, the presence of steep climatic gradients associated with the current Arctic coastline and the prevalence of organic soils, it is possible that rates of treeline expansion will be regionally variable and transient forest communities with species abundances different from today's may develop.

Absorption and Modification of Lower Hybrid Waves in the Scrape Off Layer

NASA Astrophysics Data System (ADS)

Parker, R.; Wallace, G.; Shiraiwa, S.; Baek, S.-G.; Faust, I.

2015-11-01

Loss of current drive efficiency of lower hybrid waves at high density in Alcator C-Mod current drive experiments has been attributed, at least in part, to interactions in the SOL. While ray-tracing calculations indicate that collisional absorption and modification of n|| during reflections in the SOL can be significant, their validity can be called into question owing to steep SOL gradients. In order to further quantify these losses, full-wave calculations using a plane-stratified SOL model have been carried out. The results show that the loss resulting from reflections in the SOL can be substantial, with collisional losses accounting for a loss of up to 50% per bounce of the incident wave power. The loss is sensitive to the SOL parameters with the strongest collisional absorption occurring in the case of steep temperature and weak density gradients. Modification of n|| can also be significant when the density gradient and normal to the flux surfaces are not aligned. These effects are less severe for the fast wave since its penetration into the SOL is significantly less than that of the slow wave. Work supported by USDoE awards DE-FC02-99ER54512 and DE-AC02-09CH11466.

Do Mexican immigrants "import" social gradients in health to the US?

PubMed

Buttenheim, Alison; Goldman, Noreen; Pebley, Anne R; Wong, Rebeca; Chung, Chang

2010-10-01

Greater educational attainment is consistently associated with lower mortality and better health, a pattern known as the social gradient. However, recent research suggests that Mexican-origin adults in the US have weak or flat gradients, in contrast to steep gradients for non-Hispanic whites. In this study we evaluate one hypothesis for this finding: Is the relative weakness of education gradients in health behaviors observed among Mexican-origin adults in the US due to weak gradients in the sending population? We test this "imported gradients" hypothesis with data from two nationally-representative datasets: the US National Health Interview Survey (NHIS) and the Mexican National Health Survey (ENSA 2000). We compare education gradients in smoking and obesity for recently-arrived Mexican immigrants in the US to the corresponding gradients in high-migration regions of Mexico. Results partially support the imported gradients hypothesis and have implications for health education and promotion programs targeted to immigrant populations to reduce racial and ethnic disparities in health in the US.

On the feasibility to conduct gradient liquid chromatography separations in narrow-bore columns at pressures up to 2000bar.

PubMed

De Pauw, Ruben; Swier, Tim; Degreef, Bart; Desmet, Gert; Broeckhoven, Ken

2016-11-18

The limits in operating pressures are extended for narrow-bore columns in gradient elution up to 2000bar. As the required pumps for these pressures are incompatible with common chromatographic solvents and are not suitable to apply a mobile phase composition gradient, a mobile phase delivery and injection system is described and experimentally validated which allows to use any possible chromatographic solvent in isocratic and gradient elution. The mobile phase delivery and injection system also allows to perform multiple separations without the need to depressurize the column. This system consists out of 5 dual on/off valves and two large volume loops in which the gradient and equilibration volume of initial mobile phase are loaded by a commercial liquid chromatography pump. The loops are then flushed toward the column at extreme pressures. The mobile phase delivery and injection system is first evaluated in isocratic elution and shows a comparable performance to a state-of-the-art commercial flow-through-needle injector but with twice the pressure rating. Distortion of the loaded gradient by dispersion in the gradient storage loop is studied. The effect of the most important parameters (such as flow rate, pressure and gradient steepness) is experimentally investigated. Different gradient steepnesses and volumes can be applied at different flow rates and operating pressures with a good repeatability. Due to the isobaric operation of the pumps, the gradient is monitored in real-time by a mass flow meter installed at the detector outlet. The chromatograms are then converted from time to volume-base. A separation of a 19-compound sample is performed on a 300×2.1mm column at 1000bar and on a 600×2.1mm column at 2000bar. The peak capacity was found to increase from 141 to 199 and thus scales with L as is predicted by theory. This allows to conclude that the inlet pressure for narrow-bore columns in gradient elution can be increased up to 2000bar without fundamental pressure-induced limitations. Copyright © 2016 Elsevier B.V. All rights reserved.

Maintaining microendemic primate species along an environmental gradient – parasites as drivers for species differentiation

PubMed Central

Sommer, Simone; Rakotondranary, Solofomalla Jacques; Ganzhorn, Jörg U

2014-01-01

Understanding the drivers of species adaptations to changing environments on the one hand and the limits for hybridization on the other hand is among the hottest questions in evolutionary biology. Parasites represent one of the major selective forces driving host evolution and at least those with free-living stages are at the same time dependent on the ecological conditions of their host's habitat. Local immunological adaptations of host species to varying parasite pressure are therefore expected and might represent the genetic basis for ecological speciation and the maintenance of recently diverged species. Madagascar provides one of the rare examples where two partially sympatric primate species (Microcebus griseorufus, M. murinus) and their hybrids, as well as an allopatric species (M. cf rufus) live in close proximity along a very steep environmental gradient ranging from southern dry spiny bush to gallery forest to evergreen eastern humid rain forest, thus mimicking the situation encountered during extensions and retreats of vegetation formations under changing climatic conditions. This system was used to study parasite infection and immune gene (MHC) adaptations to varying parasite pressure that might provide selective advantages to pure species over hybrids. Parasite burdens increased with increasing humidity. M. griseorufus, M. murinus, and their hybrids but not M. rufus shared the same MHC alleles, indicating either retention of ancestral polymorphism or recent gene flow. The hybrids had much higher prevalence of intestinal parasites than either of the parent species living under identical environmental conditions. The different representation of parasites can indicate a handicap for hybrids that maintains species identities. PMID:25558366

Maintaining microendemic primate species along an environmental gradient - parasites as drivers for species differentiation.

PubMed

Sommer, Simone; Rakotondranary, Solofomalla Jacques; Ganzhorn, Jörg U

2014-12-01

Understanding the drivers of species adaptations to changing environments on the one hand and the limits for hybridization on the other hand is among the hottest questions in evolutionary biology. Parasites represent one of the major selective forces driving host evolution and at least those with free-living stages are at the same time dependent on the ecological conditions of their host's habitat. Local immunological adaptations of host species to varying parasite pressure are therefore expected and might represent the genetic basis for ecological speciation and the maintenance of recently diverged species. Madagascar provides one of the rare examples where two partially sympatric primate species (Microcebus griseorufus, M. murinus) and their hybrids, as well as an allopatric species (M. cf rufus) live in close proximity along a very steep environmental gradient ranging from southern dry spiny bush to gallery forest to evergreen eastern humid rain forest, thus mimicking the situation encountered during extensions and retreats of vegetation formations under changing climatic conditions. This system was used to study parasite infection and immune gene (MHC) adaptations to varying parasite pressure that might provide selective advantages to pure species over hybrids. Parasite burdens increased with increasing humidity. M. griseorufus, M. murinus, and their hybrids but not M. rufus shared the same MHC alleles, indicating either retention of ancestral polymorphism or recent gene flow. The hybrids had much higher prevalence of intestinal parasites than either of the parent species living under identical environmental conditions. The different representation of parasites can indicate a handicap for hybrids that maintains species identities.

Towards an integrated soil moisture drought monitor for East Africa

USDA-ARS?s Scientific Manuscript database

Drought in East Africa is a recurring phenomenon with significant humanitarian impacts. Given the steep climatic gradients, topographic contrasts, general data scarcity, and, in places, political instability that characterize the region, there is a need for spatially distributed, remotely derived mo...

Characterization of the Navy Fan Channel-to-Lobe Transition: Geomorphology, Gradient, and Structure Imaged through High-Resolution AUV Bathymetry

NASA Astrophysics Data System (ADS)

Carvajal, C.; Paull, C. K.; Caress, D. W.; Anderson, K.; Lundsten, E. M.; Gwiazda, R.; Fildani, A.; Dykstra, M.; McGann, M.; Maier, K. L.; Herguera, J. C.

2016-12-01

Channel to lobe transition zones (CLTZ) are elusive sectors of the seafloor. They record complex interactions between sediment-gravity flows, flow confinement, and gradient that can result in contrasting geomorphologies. If present, structural controls can add additional intricacies. We illustrate such complexities in the Navy Fan CLTZ offshore California/Mexico using AUV-collected high-resolution (1x1x0.25 m) bathymetry and chirp profiles. The AUV bathymetry images the fine scale details of the seafloor, otherwise unresolved in surface-ship-mounted multibeam bathymetry. Three morphological areas standout that in a direction transverse to sediment transport are: 1) An unconfined area with variable but overall steep gradients (0.5o-1.7o), and considerable erosion shown by numerous large scours that truncate underlying strata. These scours are elongate (<500x180 m), deep (<18 m), asymmetric (steeper proximally), and more prominent along steeper gradients; 2) An area of moderate confinement along a smoother, gentler gradient (0.2o-0.5o; 0.9o locally). This area is 8 km long with a channel (WxD: 233x11 m) that transitions basinwards to low confinement (WxD: 1000x4 m); and 3) An area with an escarpment (<25 m high, <19o) and ridge of the San Clemente Fault. We hypothesize that the erosional morphologies of the unconfined areas reflect swifter turbidity currents due to high gradients, which resulted from relief along the San Clemente Fault and probably from differential seafloor aggradation. In the moderate confinement area, the smoother and gentler seafloor may be related to more efficient sediment dispersal able to transfer/deposit sediment to heal structural relief (though not completely) while avoiding significant local aggradation, hence preventing major gradient build up. In the faulted area, the steep and prominent structure reroutes the sediments. The findings of this study have broad application to any seafloor areas with rapid changes of gradient.

The GALAH survey: properties of the Galactic disc(s) in the solar neighbourhood

NASA Astrophysics Data System (ADS)

Duong, L.; Freeman, K. C.; Asplund, M.; Casagrande, L.; Buder, S.; Lind, K.; Ness, M.; Bland-Hawthorn, J.; De Silva, G. M.; D'Orazi, V.; Kos, J.; Lewis, G. F.; Lin, J.; Martell, S. L.; Schlesinger, K.; Sharma, S.; Simpson, J. D.; Zucker, D. B.; Zwitter, T.; Anguiano, B.; Da Costa, G. S.; Hyde, E.; Horner, J.; Kafle, P. R.; Nataf, D. M.; Reid, W.; Stello, D.; Ting, Y.-S.; Wyse, R. F. G.

2018-06-01

Using data from the GALAH pilot survey, we determine properties of the Galactic thin and thick discs near the solar neighbourhood. The data cover a small range of Galactocentric radius (7.9 ≲ R_GC ≲ 9.5 kpc), but extend up to 4 kpc in height from the Galactic plane, and several kpc in the direction of Galactic anti-rotation (at longitude 260° ≤ ℓ ≤ 280°). This allows us to reliably measure the vertical density and abundance profiles of the chemically and kinematically defined `thick' and `thin' discs of the Galaxy. The thin disc (low-α population) exhibits a steep negative vertical metallicity gradient, at d[M/H]/dz = -0.18 ± 0.01 dex kpc-1, which is broadly consistent with previous studies. In contrast, its vertical α-abundance profile is almost flat, with a gradient of d[α/M]/dz = 0.008 ± 0.002 dex kpc-1. The steep vertical metallicity gradient of the low-α population is in agreement with models where radial migration has a major role in the evolution of the thin disc. The thick disc (high-α population) has a weaker vertical metallicity gradient d[M/H]/dz = -0.058 ± 0.003 dex kpc-1. The α-abundance of the thick disc is nearly constant with height, d[α/M]/dz = 0.007 ± 0.002 dex kpc-1. The negative gradient in metallicity and the small gradient in [α/M] indicate that the high-α population experienced a settling phase, but also formed prior to the onset of major Type Ia supernova enrichment. We explore the implications of the distinct α-enrichments and narrow [α/M] range of the sub-populations in the context of thick disc formation.

Do Mexican immigrants “import” social gradients in health to the US?

PubMed Central

Buttenheim, Alison; Goldman, Noreen; Pebley, Anne R; Wong, Rebeca; Chung, Chang

2011-01-01

Greater educational attainment is consistently associated with lower mortality and better health, a pattern known as the social gradient. However, recent research suggests that Mexican-origin adults in the US have weak or flat gradients, in contrast to steep gradients for non-Hispanic whites. In this study we evaluate one hypothesis for this finding: Is the relative weakness of education gradients in health behaviors observed among Mexican-origin adults in the US due to weak gradients in the sending population? We test this “imported gradients” hypothesis with data from two nationally-representative datasets: the US National Health Interview Survey (NHIS) and the Mexican National Health Survey (ENSA 2000). We compare education gradients in smoking and obesity for recently-arrived Mexican immigrants in the US to the corresponding gradients in high-migration regions of Mexico. Results partially support the imported gradients hypothesis and have implications for health education and promotion programs targeted to immigrant populations to reduce racial and ethnic disparities in health in the US. PMID:20692753

Freshwater biodissolution rates of limestone in the temperate climate of the Dinaric karst in Slovenia

NASA Astrophysics Data System (ADS)

Mulec, J.; Prelovšek, M.

2015-01-01

Dissolution rates in two freshwater karst systems were determined by using tablets of dense micrite-biopelmicrite Cretaceous limestone. Submerged limestone tablets in riverbeds were subjected to a natural gradient from complete darkness to direct sunlight. Higher light rates significantly (p < 0.05) increased the epilithic biomass of phototrophs and the overall dissolution rates, which were highest at the Unica spring (- 49.2 μm a- 1), but the exact portion of light-dependent dissolution remains elusive. In the karst river Unica, with its big fluctuations in environmental parameters (e.g., discharge), light rates can be used in estimating the dissolution rates enhanced by phototrophs. Natural biofilms in aquatic systems have important implications for landform evolution, and the impact on limestone dissolution rates is comparable with rates of debris falling from steep slopes.

Speed and thickness of the magnetopause.

NASA Technical Reports Server (NTRS)

Kaufmann, R. L.; Konradi, A.

1973-01-01

We have used the finite gyroradius of protons with energies greater than 140 keV to determine the location of the magnetopause when the satellite is within the adjacent steep proton flux gradient. This steep gradient region is usually two to four 140-keV proton gyroradii, or about 1000 to 4000 km thick. The measurements described here were made within 45 deg of the earth-sun line on moderately disturbed days, when proton fluxes were unusually high. On these days, the magnetopause usually moves at a speed of less than 20 km/sec. The magnetopause velocity sometimes changes abruptly, while remaining below 20 km/sec. Very rapid (about 50 km/sec) radial motion appears to be associated with the propagation of single, isolated waves along the magnetopause. The thickness of the electric current sheet that produces the magnetic field rotation in the magnetopause is usually on the order of 10 times the gyroradius of a 1-keV proton, or about 1000 km.

Measurement of deuterium density profiles in the H-mode steep gradient region using charge exchange recombination spectroscopy on DIII-D

DOE PAGES

Haskey, S. R.; Grierson, B. A.; Burrell, K. H.; ...

2016-09-26

Recent completion of a thirty two channel main-ion (deuterium) charge exchange recombination spectroscopy (CER) diagnostic on the DIII-D tokamak enables detailed comparisons between impurity and main-ion temperature, density, and toroidal rotation. In a H-mode DIII-D discharge, these new measurement capabilities are used to provide the deuterium density profile, demonstrate the importance of profile alignment between Thomson scattering and CER diagnostics, and aid in determining the electron temperature at the separatrix. Sixteen sightlines cover the core of the plasma and another sixteen are densely packed towards the plasma edge, providing high resolution measurements across the pedestal and steep gradient region inmore » H-mode plasmas. Extracting useful physical quantities such as deuterium density is challenging due to multiple photoemission processes. Finally, these challenges are overcome using a detailed fitting model and by forward modeling the photoemission using the FIDASIM code, which implements a comprehensive collisional radiative model. Published by AIP Publishing.« less

Preliminary isostatic residual gravity map of the Tremonton 30' x 60' quadrangle, Box Elder and Cache Counties, Utah, and Franklin and Oneida Counties, Idaho

USGS Publications Warehouse

Langenheim, Victoria; Oaks, R.Q.; Willis, H.; Hiscock, A.I.; Chuchel, Bruce A.; Rosario, Jose J.; Hardwick, C.L.

2014-01-01

A new isostatic residual gravity map of the Tremonton 30' x 60' quadrangle of Utah is based on compilation of preexisting data and new data collected by the Utah and U.S. Geological Surveys. Pronounced gravity lows occur over North Bay, northwest of Brigham City, and Malad and Blue Creek Valleys, indicating significant thickness of low-density Tertiary sedimentary rocks and deposits. Gravity highs coincide with exposures of dense pre-Cenozoic rocks in the Promontory, Clarkston, and Wellsville Mountains. The highest gravity values are located in southern Curlew Valley and may be produced in part by deeper crustal density variations or crustal thinning. Steep, linear gravity gradients coincide with Quaternary faults bounding the Wellsville and Clarkston Mountains. Steep gradients also coincide with the margins of the Promontory Mountains, Little Mountain, West Hills, and the eastern margin of the North Promontory Mountains and may define concealed basin-bounding faults.

Measurement of deuterium density profiles in the H-mode steep gradient region using charge exchange recombination spectroscopy on DIII-D.

PubMed

Haskey, S R; Grierson, B A; Burrell, K H; Chrystal, C; Groebner, R J; Kaplan, D H; Pablant, N A; Stagner, L

2016-11-01

Recent completion of a thirty two channel main-ion (deuterium) charge exchange recombination spectroscopy (CER) diagnostic on the DIII-D tokamak [J. L. Luxon, Nucl. Fusion 42, 614 (2002)] enables detailed comparisons between impurity and main-ion temperature, density, and toroidal rotation. In a H-mode DIII-D discharge, these new measurement capabilities are used to provide the deuterium density profile, demonstrate the importance of profile alignment between Thomson scattering and CER diagnostics, and aid in determining the electron temperature at the separatrix. Sixteen sightlines cover the core of the plasma and another sixteen are densely packed towards the plasma edge, providing high resolution measurements across the pedestal and steep gradient region in H-mode plasmas. Extracting useful physical quantities such as deuterium density is challenging due to multiple photoemission processes. These challenges are overcome using a detailed fitting model and by forward modeling the photoemission using the FIDASIM code, which implements a comprehensive collisional radiative model.

Measurement of deuterium density profiles in the H-mode steep gradient region using charge exchange recombination spectroscopy on DIII-D

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haskey, S. R.; Grierson, B. A.; Burrell, K. H.

Recent completion of a thirty two channel main-ion (deuterium) charge exchange recombination spectroscopy (CER) diagnostic on the DIII-D tokamak enables detailed comparisons between impurity and main-ion temperature, density, and toroidal rotation. In a H-mode DIII-D discharge, these new measurement capabilities are used to provide the deuterium density profile, demonstrate the importance of profile alignment between Thomson scattering and CER diagnostics, and aid in determining the electron temperature at the separatrix. Sixteen sightlines cover the core of the plasma and another sixteen are densely packed towards the plasma edge, providing high resolution measurements across the pedestal and steep gradient region inmore » H-mode plasmas. Extracting useful physical quantities such as deuterium density is challenging due to multiple photoemission processes. Finally, these challenges are overcome using a detailed fitting model and by forward modeling the photoemission using the FIDASIM code, which implements a comprehensive collisional radiative model. Published by AIP Publishing.« less

Measurement of deuterium density profiles in the H-mode steep gradient region using charge exchange recombination spectroscopy on DIII-D

NASA Astrophysics Data System (ADS)

Haskey, S. R.; Grierson, B. A.; Burrell, K. H.; Chrystal, C.; Groebner, R. J.; Kaplan, D. H.; Pablant, N. A.; Stagner, L.

2016-11-01

Recent completion of a thirty two channel main-ion (deuterium) charge exchange recombination spectroscopy (CER) diagnostic on the DIII-D tokamak [J. L. Luxon, Nucl. Fusion 42, 614 (2002)] enables detailed comparisons between impurity and main-ion temperature, density, and toroidal rotation. In a H-mode DIII-D discharge, these new measurement capabilities are used to provide the deuterium density profile, demonstrate the importance of profile alignment between Thomson scattering and CER diagnostics, and aid in determining the electron temperature at the separatrix. Sixteen sightlines cover the core of the plasma and another sixteen are densely packed towards the plasma edge, providing high resolution measurements across the pedestal and steep gradient region in H-mode plasmas. Extracting useful physical quantities such as deuterium density is challenging due to multiple photoemission processes. These challenges are overcome using a detailed fitting model and by forward modeling the photoemission using the FIDASIM code, which implements a comprehensive collisional radiative model.

Wetlands of the Prairie Pothole Region: Invertebrate species composition, ecology, and management

USGS Publications Warehouse

Euliss, N.H.; Wrubleski, D.A.; Mushet, D.M.; Batzer, D.P.; Rader, R.B.; Wissinger, S.A.

1999-01-01

The Prairie Pothole Region (PPR) of the United States and Canada is a unique area where shallow depressions created by the scouring action of Pleistocene glaciation interact with mid-continental climate variations to create and maintain a variety of wetland classes. These wetlands possess unique environmental and biotic characteristics that add to the overall regional diversity and production of aquatic invertebrates and the vertebrate wildlife that depend upon them as food. Climatic extremes in the PPR have a profound and dynamic influence on wetland hydrology, hydroperiod, chemistry, and ultimately the biota. Available knowledge of aquatic invertebrates in the PPR suggests that diversity of invertebrates within each wetland class is low. Harsh environmental conditions range from frigid winter temperatures that freeze wetlands and their sediments to hot summer temperatures and drought conditions that create steep salinity gradients and seasonally dry habitats. Consequently, the invertebrate community is composed mostly of ecological generalists that possess the necessary adaptations to tolerate environmental extremes. In this review, we describe the highly dynamic nature of prairie pothole wetlands and suggest that invertebrate studies be evaluated within a conceptual framework that considers important hydrologic, chemical, and climatic events.

Acclimatization of symbiotic corals to mesophotic light environments through wavelength transformation by fluorescent protein pigments

PubMed Central

D'Angelo, Cecilia; Sharon, Yoni; Tchernov, Dan; Wiedenmann, Joerg

2017-01-01

The depth distribution of reef-building corals exposes their photosynthetic symbionts of the genus Symbiodinium to extreme gradients in the intensity and spectral quality of the ambient light environment. Characterizing the mechanisms used by the coral holobiont to respond to the low intensity and reduced spectral composition of the light environment in deeper reefs (greater than 20 m) is fundamental to our understanding of the functioning and structure of reefs across depth gradients. Here, we demonstrate that host pigments, specifically photoconvertible red fluorescent proteins (pcRFPs), can promote coral adaptation/acclimatization to deeper-water light environments by transforming the prevalent blue light into orange-red light, which can penetrate deeper within zooxanthellae-containing tissues; this facilitates a more homogeneous distribution of photons across symbiont communities. The ecological importance of pcRFPs in deeper reefs is supported by the increasing proportion of red fluorescent corals with depth (measured down to 45 m) and increased survival of colour morphs with strong expression of pcRFPs in long-term light manipulation experiments. In addition to screening by host pigments from high light intensities in shallow water, the spectral transformation observed in deeper-water corals highlights the importance of GFP-like protein expression as an ecological mechanism to support the functioning of the coral–Symbiodinium association across steep environmental gradients. PMID:28679724

Acclimatization of symbiotic corals to mesophotic light environments through wavelength transformation by fluorescent protein pigments.

PubMed

Smith, Edward G; D'Angelo, Cecilia; Sharon, Yoni; Tchernov, Dan; Wiedenmann, Joerg

2017-07-12

The depth distribution of reef-building corals exposes their photosynthetic symbionts of the genus Symbiodinium to extreme gradients in the intensity and spectral quality of the ambient light environment. Characterizing the mechanisms used by the coral holobiont to respond to the low intensity and reduced spectral composition of the light environment in deeper reefs (greater than 20 m) is fundamental to our understanding of the functioning and structure of reefs across depth gradients. Here, we demonstrate that host pigments, specifically photoconvertible red fluorescent proteins (pcRFPs), can promote coral adaptation/acclimatization to deeper-water light environments by transforming the prevalent blue light into orange-red light, which can penetrate deeper within zooxanthellae-containing tissues; this facilitates a more homogeneous distribution of photons across symbiont communities. The ecological importance of pcRFPs in deeper reefs is supported by the increasing proportion of red fluorescent corals with depth (measured down to 45 m) and increased survival of colour morphs with strong expression of pcRFPs in long-term light manipulation experiments. In addition to screening by host pigments from high light intensities in shallow water, the spectral transformation observed in deeper-water corals highlights the importance of GFP-like protein expression as an ecological mechanism to support the functioning of the coral- Symbiodinium association across steep environmental gradients. © 2017 The Authors.

Mountain Plant Community Sentinels: AWOL

NASA Astrophysics Data System (ADS)

Malanson, G. P.

2017-12-01

Mountain plant communities are thought to be sensitive to climate change. Because climatic gradients are steep on mountain slopes, the spatial response of plant communities to climate change should be compressed and easier to detect. These expectations have led to identifying mountain plant communities as sentinels for climate change. This idea has, however, been criticized. Two critiques, for alpine treeline and alpine tundra, are rehearsed and supplemented. The critique of alpine treeline as sentinel is bolstered with new model results on the confounding role of dispersal mechanisms and sensitivity to climatic volatility. In alpine tundra, for which background turnover rates have yet to be established, community composition may reflect environmental gradients only for extremes where effects of climate are most indirect. Both plant communities, while primarily determined by energy at broad scales, may respond to water as a proximate driver at local scales. These plant communities may not be in equilibrium with climate, and differently scaled time lags may mean that ongoing vegetation change may not signal ongoing climate change (or lack thereof). In both cases a double-whammy is created by scale dependence for time lags and for drivers leading to confusion, but these cases present opportunities for insights into basic ecology.

Cognitive Tests in Early Childhood: Psychometric and Cultural Considerations

ERIC Educational Resources Information Center

Williams, Marian E.; Sando, Lara; Soles, Tamara Glen

2014-01-01

Cognitive assessment of young children contributes to high-stakes decisions because results are often used to determine eligibility for early intervention and special education. Previous reviews of cognitive measures for young children highlighted concerns regarding adequacy of standardization samples, steep item gradients, and insufficient floors…

Multiple zonal jets and convective heat transport barriers in a quasi-geostrophic model of planetary cores

NASA Astrophysics Data System (ADS)

Guervilly, C.; Cardin, P.

2017-10-01

We study rapidly rotating Boussinesq convection driven by internal heating in a full sphere. We use a numerical model based on the quasi-geostrophic approximation for the velocity field, whereas the temperature field is 3-D. This approximation allows us to perform simulations for Ekman numbers down to 10-8, Prandtl numbers relevant for liquid metals (˜10-1) and Reynolds numbers up to 3 × 104. Persistent zonal flows composed of multiple jets form as a result of the mixing of potential vorticity. For the largest Rayleigh numbers computed, the zonal velocity is larger than the convective velocity despite the presence of boundary friction. The convective structures and the zonal jets widen when the thermal forcing increases. Prograde and retrograde zonal jets are dynamically different: in the prograde jets (which correspond to weak potential vorticity gradients) the convection transports heat efficiently and the mean temperature tends to be homogenized; by contrast, in the cores of the retrograde jets (which correspond to steep gradients of potential vorticity) the dynamics is dominated by the propagation of Rossby waves, resulting in the formation of steep mean temperature gradients and the dominance of conduction in the heat transfer process. Consequently, in quasi-geostrophic systems, the width of the retrograde zonal jets controls the efficiency of the heat transfer.

Distinguishing between debris flows and floods from field evidence in small watersheds

USGS Publications Warehouse

Pierson, Thomas C.

2005-01-01

Post-flood indirect measurement techniques to back-calculate flood magnitude are not valid for debris flows, which commonly occur in small steep watersheds during intense rainstorms. This is because debris flows can move much faster than floods in steep channel reaches and much slower than floods in low-gradient reaches. In addition, debris-flow deposition may drastically alter channel geometry in reaches where slope-area surveys are applied. Because high-discharge flows are seldom witnessed and automated samplers are commonly plugged or destroyed, determination of flow type often must be made on the basis of field evidence preserved at the site.

Arbuscular mycorrhizal fungal communities are phylogenetically clustered at small scales

PubMed Central

Horn, Sebastian; Caruso, Tancredi; Verbruggen, Erik; Rillig, Matthias C; Hempel, Stefan

2014-01-01

Next-generation sequencing technologies with markers covering the full Glomeromycota phylum were used to uncover phylogenetic community structure of arbuscular mycorrhizal fungi (AMF) associated with Festuca brevipila. The study system was a semi-arid grassland with high plant diversity and a steep environmental gradient in pH, C, N, P and soil water content. The AMF community in roots and rhizosphere soil were analyzed separately and consisted of 74 distinct operational taxonomic units (OTUs) in total. Community-level variance partitioning showed that the role of environmental factors in determining AM species composition was marginal when controlling for spatial autocorrelation at multiple scales. Instead, phylogenetic distance and spatial distance were major correlates of AMF communities: OTUs that were more closely related (and which therefore may have similar traits) were more likely to co-occur. This pattern was insensitive to phylogenetic sampling breadth. Given the minor effects of the environment, we propose that at small scales closely related AMF positively associate through biotic factors such as plant-AMF filtering and interactions within the soil biota. PMID:24824667

Metacommunity analysis of amoeboid protists in grassland soils

PubMed Central

Fiore-Donno, Anna Maria; Weinert, Jan; Wubet, Tesfaye; Bonkowski, Michael

2016-01-01

This study reveals the diversity and distribution of two major ubiquitous groups of soil amoebae, the genus Acanthamoeba and the Myxomycetes (plasmodial slime-moulds) that are rarely, if ever, recovered in environmental sampling studies. We analyzed 150 grassland soil samples from three Biodiversity Exploratories study regions in Germany. We developed specific primers targeting the V2 variable region in the first part of the small subunit of the ribosomal RNA gene for high-throughput pyrotag sequencing. From ca. 1 million reads, applying very stringent filtering and clustering parameters to avoid overestimation of the diversity, we obtained 273 acanthamoebal and 338 myxomycete operational taxonomic units (OTUs, 96% similarity threshold). This number is consistent with the genetic diversity known in the two investigated lineages, but unequalled to date by any environmental sampling study. Only very few OTUs were identical to already known sequences. Strikingly different OTUs assemblages were found between the three German regions (PerMANOVA p.value = 0.001) and even between sites of the same region (multiple-site Simpson-based similarity indices <0.4), showing steep biogeographical gradients. PMID:26750872

Metacommunity analysis of amoeboid protists in grassland soils.

PubMed

Fiore-Donno, Anna Maria; Weinert, Jan; Wubet, Tesfaye; Bonkowski, Michael

2016-01-11

This study reveals the diversity and distribution of two major ubiquitous groups of soil amoebae, the genus Acanthamoeba and the Myxomycetes (plasmodial slime-moulds) that are rarely, if ever, recovered in environmental sampling studies. We analyzed 150 grassland soil samples from three Biodiversity Exploratories study regions in Germany. We developed specific primers targeting the V2 variable region in the first part of the small subunit of the ribosomal RNA gene for high-throughput pyrotag sequencing. From ca. 1 million reads, applying very stringent filtering and clustering parameters to avoid overestimation of the diversity, we obtained 273 acanthamoebal and 338 myxomycete operational taxonomic units (OTUs, 96% similarity threshold). This number is consistent with the genetic diversity known in the two investigated lineages, but unequalled to date by any environmental sampling study. Only very few OTUs were identical to already known sequences. Strikingly different OTUs assemblages were found between the three German regions (PerMANOVA p.value = 0.001) and even between sites of the same region (multiple-site Simpson-based similarity indices <0.4), showing steep biogeographical gradients.

The Footprint of Continental-Scale Ocean Currents on the Biogeography of Seaweeds

PubMed Central

Wernberg, Thomas; Thomsen, Mads S.; Connell, Sean D.; Russell, Bayden D.; Waters, Jonathan M.; Zuccarello, Giuseppe C.; Kraft, Gerald T.; Sanderson, Craig; West, John A.; Gurgel, Carlos F. D.

2013-01-01

Explaining spatial patterns of biological organisation remains a central challenge for biogeographic studies. In marine systems, large-scale ocean currents can modify broad-scale biological patterns by simultaneously connecting environmental (e.g. temperature, salinity and nutrients) and biological (e.g. amounts and types of dispersed propagules) properties of adjacent and distant regions. For example, steep environmental gradients and highly variable, disrupted flow should lead to heterogeneity in regional communities and high species turnover. In this study, we investigated the possible imprint of the Leeuwin (LC) and East Australia (EAC) Currents on seaweed communities across ~7,000 km of coastline in temperate Australia. These currents flow poleward along the west and east coasts of Australia, respectively, but have markedly different characteristics. We tested the hypothesis that, regional seaweed communities show serial change in the direction of current flow and that, because the LC is characterised by a weaker temperature gradient and more un-interrupted along-shore flow compared to the EAC, then coasts influenced by the LC have less variable seaweed communities and lower species turnover across regions than the EAC. This hypothesis was supported. We suggest that this pattern is likely caused by a combination of seaweed temperature tolerances and current-driven dispersal. In conclusion, our findings support the idea that the characteristics of continental-scale currents can influence regional community organisation, and that the coupling of ocean currents and marine biological structure is a general feature that transcends taxa and spatial scales. PMID:24260352

40 CFR 230.45 - Riffle and pool complexes.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Riffle and pool complexes. 230.45... Impacts on Special Aquatic Sites § 230.45 Riffle and pool complexes. (a) Steep gradient sections of streams are sometimes characterized by riffle and pool complexes. Such stream sections are recognizable by...

40 CFR 230.45 - Riffle and pool complexes.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Riffle and pool complexes. 230.45... Impacts on Special Aquatic Sites § 230.45 Riffle and pool complexes. (a) Steep gradient sections of streams are sometimes characterized by riffle and pool complexes. Such stream sections are recognizable by...

Topographic separation of two sympatric palms in the central Amazon - does dispersal play a role?

NASA Astrophysics Data System (ADS)

Gomes de Freitas, Cintia; Capellotto Costa, Flávia Regina; Svenning, Jens-Christian; Balslev, Henrik

2012-02-01

Despite broadly overlapping geographic distributions in the central Amazon basin, two congeneric palm species (Attalea attaleoides and Attalea microcarpa) have topographically separated distributions on a local scale in Reserva Ducke near Manaus. Our aim here was to determine if this local scale separation can be linked to (1) seedling stage specialization to different habitat conditions of the two species, and/or (2) environmentally-controlled seed dispersal. We assessed the role of these potential drivers by mapping the local distribution of the two species over a 25-km2 grid and testing for correlation to seed removal and seed germination patterns using seed sowing experiments. 360 seeds of each species were sown in 30 uniformly distributed plots (12 seeds of each species in each plot), and seed removal and germination were subsequently monitored. Adult populations of the two species showed opposite distribution patterns linked to topography. However, there was little evidence for specialization to different habitat conditions at the seedling stage: after 11 months, 26.1% of seeds of A. microcarpa had germinated along the entire topographic gradient, albeit with a tendency toward higher germination in more inclined areas. For A. attaleoides, only 2.2% seeds had germinated, and again along the entire topographic gradient. In contrast, there was evidence for environmentally-controlled seed dispersal: for both species, seed removal was higher in flat areas. Presence of adults did not affect germination or seed removal. Our results suggest that topographically differentiated distributions of A. attaleoides and A. microcarpa may be reinforced by steep slope avoidance by their seed dispersers. A direct environmental control mechanism remains to be identified to explain the consistent topographic associations, but our results show that this mechanism does not work at the seed germination stage.

Orographic Barriers, Rainshadows, and Earth Surface Processes in the Central Andes

NASA Astrophysics Data System (ADS)

Bookhagen, B.; Strecker, M. R.

2016-12-01

The Central Andes of NW Argentina, northern Chile, and SW Bolivia are characterized by a steep E-W topographic, climatic and environmental gradient. The first windward topographic rise in the eastern Central Andes forces high orographic rainfall and dense vegetation. In contrast, the higher-elevation areas of the windward flanks become progressively drier, until arid conditions are attained in the orogen interior. On seasonal, annual, and inter-annual timescales, large rainstorms may propagate into the semi-arid to arid high-elevation sectors and cause erosion and mass-transport processes that impact infrastructure and the natural environment. Similar to these present-day effects of climate variability the Central Andes experienced pronounced paleoclimatic changes with deeper penetration of moisture into the orogen and thus an orogenward shift of the climate gradient during Pleistocene and Holocene times, lasting several millennia. In this presentation, we demonstrate the impact of climate change on Earth surface processes at different timescales ranging from the late Pleistocene to the past decade. For millennial timescales and beyond, we rely on field observations, dating of geomorphic markers, erosion rates from cosmogenic nuclide dating, and the analysis of sedimentary archives to reconstruct past environmental conditions. For the last decades we use, satellite-derived rainfall and landcover observations, climate models, hydrometeorologic data, and riverbed-elevation changes are used to characterize environmental and atmospheric conditions. Decadal-scale climate variability shows statistically significant hydrometeorologic trends and exhibits changes of fluvial-transport magnitudes. Hydrometeorologic data, their trends and change points suggest that highest rainfall magnitudes have increased most in the past decades, resulting in large, event-driven mass-transport processes with fundamental impacts on population and infrastructure.

The changing hydro-ecological dynamics of rivers and deltas of the Western Indian Ocean: Anthropogenic and environmental drivers, local adaptation and policy response

NASA Astrophysics Data System (ADS)

Duvail, Stéphanie; Hamerlynck, Olivier; Paron, Paolo; Hervé, Dominique; Nyingi, Wanja D.; Leone, Michele

2017-10-01

The rivers flowing into the Western Indian Ocean have steep headwater gradients and carry high sediment loads. In combination with strong tides and seasonal rainfall, these rivers create dynamic deltas with biodiversity-rich and productive ecosystems that, through flooding, have sustained indigenous use systems for centuries. However, river catchments are rapidly changing due to deforestation. Hydropower dams also increasingly alter flood characteristics, reduce sediment supply and contribute to coastal erosion. These impacts are compounded by climate change. Altogether, these changes affect the livelihoods of the delta users. Here, based on prior works that we and others have conducted in the region, we analyse the drivers of these hydro-ecological changes. We then provide recommendations for improved dam design and operations to sustain the underlying delta-building processes, the ecosystem values and the needs of the users.

Pervasive phosphorus limitation of tree species but not communities in tropical forests

NASA Astrophysics Data System (ADS)

Turner, Benjamin L.; Brenes-Arguedas, Tania; Condit, Richard

2018-03-01

Phosphorus availability is widely assumed to limit primary productivity in tropical forests, but support for this paradigm is equivocal. Although biogeochemical theory predicts that phosphorus limitation should be prevalent on old, strongly weathered soils, experimental manipulations have failed to detect a consistent response to phosphorus addition in species-rich lowland tropical forests. Here we show, by quantifying the growth of 541 tropical tree species across a steep natural phosphorus gradient in Panama, that phosphorus limitation is widespread at the level of individual species and strengthens markedly below a threshold of two parts per million exchangeable soil phosphate. However, this pervasive species-specific phosphorus limitation does not translate into a community-wide response, because some species grow rapidly on infertile soils despite extremely low phosphorus availability. These results redefine our understanding of nutrient limitation in diverse plant communities and have important implications for attempts to predict the response of tropical forests to environmental change.

Oxygen sensing and signaling.

PubMed

van Dongen, Joost T; Licausi, Francesco

2015-01-01

Oxygen is an indispensable substrate for many biochemical reactions in plants, including energy metabolism (respiration). Despite its importance, plants lack an active transport mechanism to distribute oxygen to all cells. Therefore, steep oxygen gradients occur within most plant tissues, which can be exacerbated by environmental perturbations that further reduce oxygen availability. Plants possess various responses to cope with spatial and temporal variations in oxygen availability, many of which involve metabolic adaptations to deal with energy crises induced by low oxygen. Responses are induced gradually when oxygen concentrations decrease and are rapidly reversed upon reoxygenation. A direct effect of the oxygen level can be observed in the stability, and thus activity, of various transcription factors that control the expression of hypoxia-induced genes. Additional signaling pathways are activated by the impact of oxygen deficiency on mitochondrial and chloroplast functioning. Here, we describe the molecular components of the oxygen-sensing pathway.

Observations of wave-induced pore pressure gradients and bed level response on a surf zone sandbar

NASA Astrophysics Data System (ADS)

Anderson, Dylan; Cox, Dan; Mieras, Ryan; Puleo, Jack A.; Hsu, Tian-Jian

2017-06-01

Horizontal and vertical pressure gradients may be important physical mechanisms contributing to onshore sediment transport beneath steep, near-breaking waves in the surf zone. A barred beach was constructed in a large-scale laboratory wave flume with a fixed profile containing a mobile sediment layer on the crest of the sandbar. Horizontal and vertical pore pressure gradients were obtained by finite differences of measurements from an array of pressure transducers buried within the upper several centimeters of the bed. Colocated observations of erosion depth were made during asymmetric wave trials with wave heights between 0.10 and 0.98 m, consistently resulting in onshore sheet flow sediment transport. The pore pressure gradient vector within the bed exhibited temporal rotations during each wave cycle, directed predominantly upward under the trough and then rapidly rotating onshore and downward as the wavefront passed. The magnitude of the pore pressure gradient during each phase of rotation was correlated with local wave steepness and relative depth. Momentary bed failures as deep as 20 grain diameters were coincident with sharp increases in the onshore-directed pore pressure gradients, but occurred at horizontal pressure gradients less than theoretical critical values for initiation of the motion for compact beds. An expression combining the effects of both horizontal and vertical pore pressure gradients with bed shear stress and soil stability is used to determine that failure of the bed is initiated at nonnegligible values of both forces.