Student Attraction, Persistence and Retention in STEM Programs: Successes and Continuing Challenges

ERIC Educational Resources Information Center

Sithole, Alec; Chiyaka, Edward T.; McCarthy, Peter; Mupinga, Davison M.; Bucklein, Brian K.; Kibirige, Joachim

2017-01-01

Low student enrollment and high attrition rates in Science, Technology, Engineering, and Mathematics (STEM) education are major challenges in higher education. Many STEM entrants end-up switching their majors to non-STEM fields, perform poorly relative to their peers in other programs, and/or drop out of college without earning any academic…

Gender Gap in Science, Technology, Engineering, and Mathematics (STEM): Current Knowledge, Implications for Practice, Policy, and Future Directions

PubMed Central

Degol, Jessica L.

2016-01-01

Although the gender gap in math course-taking and performance has narrowed in recent decades, females continue to be underrepresented in math-intensive fields of Science, Technology, Engineering, and Mathematics (STEM). Career pathways encompass the ability to pursue a career as well as the motivation to employ that ability. Individual differences in cognitive capacity and motivation are also influenced by broader sociocultural factors. After reviewing research from the fields of psychology, sociology, economics, and education over the past 30 years, we summarize six explanations for US women’s underrepresentation in math-intensive STEM fields: (a) cognitive ability, (b) relative cognitive strengths, (c) occupational interests or preferences, (d) lifestyle values or work-family balance preferences, (e) field-specific ability beliefs, and (f) gender-related stereotypes and biases. We then describe the potential biological and sociocultural explanations for observed gender differences on cognitive and motivational factors and demonstrate the developmental period(s) during which each factor becomes most relevant. We then propose evidence-based recommendations for policy and practice to improve STEM diversity and recommendations for future research directions. PMID:28458499

Gender Gap in Science, Technology, Engineering, and Mathematics (STEM): Current Knowledge, Implications for Practice, Policy, and Future Directions.

PubMed

Wang, Ming-Te; Degol, Jessica L

2017-03-01

Although the gender gap in math course-taking and performance has narrowed in recent decades, females continue to be underrepresented in math-intensive fields of Science, Technology, Engineering, and Mathematics (STEM). Career pathways encompass the ability to pursue a career as well as the motivation to employ that ability. Individual differences in cognitive capacity and motivation are also influenced by broader sociocultural factors. After reviewing research from the fields of psychology, sociology, economics, and education over the past 30 years, we summarize six explanations for US women's underrepresentation in math-intensive STEM fields: (a) cognitive ability, (b) relative cognitive strengths, (c) occupational interests or preferences, (d) lifestyle values or work-family balance preferences, (e) field-specific ability beliefs, and (f) gender-related stereotypes and biases. We then describe the potential biological and sociocultural explanations for observed gender differences on cognitive and motivational factors and demonstrate the developmental period(s) during which each factor becomes most relevant. We then propose evidence-based recommendations for policy and practice to improve STEM diversity and recommendations for future research directions.

Pathways in STEM: Factors affecting the retention and attrition of talented men and women from the STEM pipeline

NASA Astrophysics Data System (ADS)

Heilbronner, Nancy N.

Many men and women who are talented in science, technology, engineering, and/or mathematics (STEM) choose not to pursue undergraduate majors or careers in these fields. To develop talents in STEM, educators must understand the factors that contribute to an individual's retention in STEM domains, as well as the factors that act as barriers to success, such as the role that gender plays in the underrepresentation of women in certain STEM fields (e.g., computer science and engineering) and changes in recent decades in the process of selecting STEM majors and careers. The purpose of this study was to explore the influences that guide decisions related to the selection of majors and occupations during high school, post-secondary education, and early careers. Survey methodology was used to explore the perceptions of 360 Science Talent Search (STS) semifinalists and finalists during the years 1987-1989 and 1997-1999, and quantitative procedures were used to analyze the data. A majority (74.2%) of STS participants majored in a STEM field in college, and most (68.6%) currently work in a STEM field. A greater percentage of men selected computer science, engineering, physics, and mathematics majors, and a greater percentage of women selected biological science and chemistry. Belief in one's ability to achieve in STEM was a predictor of STEM majors in college and STEM concentrations in graduate school, but differences were found between men's and women's self-efficacy in STEM during high school and in college, as women had lower self-efficacy. Sex was a predictor of STEM majors in college, but perceived quality of academic courses was not. STEM majors also reported more satisfaction with their STEM courses in high school and college than non-STEM majors. In a departure from the results of previous research, the reasons that men and women selected occupations were similar, as were the reasons they chose to leave or not to enter STEM. The most frequently cited reason for attrition was interest in another (non-STEM) field. Participants placed little importance on other reasons for leaving STEM that were identified in previous research, such as competition, social isolation, or financial considerations.

What's So Special about STEM? A Comparison of Women's Retention in STEM and Professional Occupations.

PubMed

Glass, Jennifer L; Sassler, Sharon; Levitte, Yael; Michelmore, Katherine M

2013-01-01

We follow female college graduates in the National Longitudinal Survey of Youth 1979 and compare the trajectories of women in science, technology, engineering, and mathematics (STEM)-related occupations to other professional occupations. Results show that women in STEM occupations are significantly more likely to leave their occupational field than professional women, especially early in their career, while few women in either group leave jobs to exit the labor force. Family factors cannot account for the differential loss of STEM workers compared to other professional workers. Few differences in job characteristics emerge either, so these cannot account for the disproportionate loss of STEM workers. What does emerge is that investments and job rewards that generally stimulate field commitment, such as advanced training and high job satisfaction, fail to build commitment among women in STEM.

Astronomy in Denver: Effects of a summer camp on girls’ preconceived notions of careers in STEM

NASA Astrophysics Data System (ADS)

Hoffman, Jennifer L.; Fetrow, Kirsten J.; Broder, Dale E.; Murphy, Shannon M.; Tinghitella, Robin; Hart, Quyen N.

2018-06-01

Despite gains in recent years, gender disparities persist in fields related to science, technology, engineering, and mathematics (STEM). Although young women can perform as well as their male peers in STEM courses and tests, they are less likely to pursue higher education and careers in STEM. Our study examined the effectiveness of a STEM-focused summer camp at increasing middle-school girls’ career aspirations in STEM and self-confidence with respect to scientific topics. The 15 participants were Denver-area girls ages 10 to 13 years old from groups underrepresented in STEM fields. During the weeklong DU SciTech camp, these girls built telescopes and computers, collected and classified insects, completed inquiry activities, and interacted with female STEM professionals from a variety of scientific fields and racial backgrounds. We hypothesized that camp attendance would expand girls’ perceptions of who does science, increase their awareness of and interest in STEM careers, and increase their scientific self-efficacy, or belief in their ability to succeed at STEM tasks. We found that DU SciTech improved the girls’ scientific self-efficacy and awareness of STEM careers, but it did not increase their (already high) interest in pursuing their own careers in STEM. We will present our results and discuss their implications for future summer camps and efforts to broaden STEM participation by young women from underrepresented groups.

Supporting Students with Disabilities Entering the Science, Technology, Engineering, and Mathematics Field Disciplines

NASA Astrophysics Data System (ADS)

Dishauzi, Karen M.

Extensive research exists on female, African American, and Hispanic students pursuing Science, Technology, Engineering and Mathematics (STEM) field disciplines. However, little research evaluates students with disabilities and career decision-making relating to STEM field disciplines. This study explored the career decision-making experiences and self-efficacy for students with disabilities. The purpose of this research study was to document experiences and perceptions of students with disabilities who pursue, and may consider pursuing, careers in the STEM field disciplines by exploring the career decision-making self-efficacy of students with disabilities. This study documented the level of influence that the students with disabilities had or may not have had encountered from parents, friends, advisors, counselors, and instructors as they managed their decision-making choice relating to their academic major/career in the STEM or non-STEM field disciplines. A total of 85 respondents of approximately 340 students with disabilities at one Midwestern public university completed a quantitatively designed survey instrument. The Career Decision-Making Self-Efficacy Scale-Short Form by Betz and Hackett was the instrument used, and additional questions were included in the survey. Data analysis included descriptive statistics and analysis of variance. Based upon the results, college students with disabilities are not currently being influenced by individuals and groups of individuals to pursue the STEM field disciplines. This is a cohort of individuals who can be marketed to increase enrollment in STEM programs at academic institutions. This research further found that gender differences at the institution under study did not affect the career decision-making self-efficacy scores. The men did not score any higher in confidence in career decision-making than the women. Disability type did not significantly affect the relationship between the Career Decision-Making Self-Efficacy Total Scores or college major choice. Of the three disability types represented more frequently, the Mental Health disability was found to be a growing disability at the institution under study. This research was found to be beneficial in the documentation of specific levels of influence perceived by students with disabilities from parents, friends, advisors, counselors, and instructors that related to their career decision-making and academic major choices.

A House Divided? Examining Persistence for On-Campus STEM and Non-STEM Students

ERIC Educational Resources Information Center

Gansemer-Topf, Ann M.; Kollasch, Aurelia; Sun, Jie

2017-01-01

Improving student persistence, especially in science, technology, engineering, and mathematics (STEM) fields, continues to be at the forefront of national educational policy discussions. Living in university housing, with its focus specifically on assisting students in transition, has consistently been positively related to student persistence.…

Dismantling the Birdcage: Adolescent Girls' Attitudes towards Learning Mathematics with a Relational Pedagogy in a Problem-Based Environment

ERIC Educational Resources Information Center

Schettino, Carmel

2013-01-01

Although the Gender Achievement Gap is closing in mathematics, the "interest gap" in pursuing STEM fields is not. Mathematics education research has discussed constructivist, student-centered and inclusive methods of teaching that have been found to encourage students that have underachieved and been underrepresented in STEM fields. One…

Attitudes about High School Physics in Relationship to Gender and Ethnicity: A Mixed Method Analysis

ERIC Educational Resources Information Center

Hafza, Rabieh Jamal

2012-01-01

There is an achievement gap and lack of participation in science, technology, engineering, and math (STEM) by minority females. The number of minority females majoring in STEM related fields and earning advanced degrees in these fields has not significantly increased over the past 40 years. Previous research has evaluated the relationship between…

Induced pluripotent stem cells--alchemist's tale or clinical reality?

PubMed

Rashid, S Tamir; Vallier, Ludovic

2010-08-13

Following Shinya Yamanaka's first report describing the reprogramming of fibroblasts into stem cells over three years ago, some sceptics initially drew analogies between this new field of research and the quasi-mystical practice of 'alchemy'. Unlike the alchemist, however, stem cell researchers have rigorously tested and repeated experiments, proving their very own brand of cellular 'alchemy' to be a reality, with potentially massive implications for the study of human biology and clinical medicine. These investigations have resulted in an explosion of related publications and initiated the field of stem cell research known as 'induced pluripotency'. In this review, we give an account of the historical development, current technologies and potential clinical applications of induced pluripotency and conclude with a perspective on the possible future directions for this dynamic field.

The missing women in STEM? Assessing gender differentials in the factors associated with transition to first jobs.

PubMed

Sassler, Sharon; Glass, Jennifer; Levitte, Yael; Michelmore, Katherine M

2017-03-01

Women remain underrepresented in the STEM workforce. We assess explanations for women's underrepresentation in STEM jobs, focusing on a cohort that came of age in the 1980s and 1990s, when women dramatically increased their representation in the scientific labor force. Data are from the NLSY79, and our analysis focuses on members of this cohort who received a college degree, with an emphasis on those who completed a degree in a STEM field. Our analyses test the extent to which college major, expectations to work in STEM, and family expectations shaped transitions into STEM occupations within two years of degree completion. Among those majoring in STEM fields there were no gender differences in transitioning into STEM jobs, though there were sizable differences in transitions to STEM employment by field of study. Of note are gender differences in associations between family expectations and transitions into STEM employment. The most career oriented women, who expected to marry late and limit fertility, were no more likely to enter STEM jobs than were women who anticipated marrying young and having two or more children. The men most likely to enter STEM occupations, in contrast, adhered to significantly more conventional gender ideologies than their female counterparts, expecting to marry at younger ages but also to remain childless. Results of our regression decomposition indicated that marriage and family expectations and gender ideology worked in opposite directions for men and women. Nonetheless, the majority of the gender disparity in transitions into STEM jobs was related to women's underrepresentation in engineering and computer science fields of study. Copyright © 2016 Elsevier Inc. All rights reserved.

The missing women in STEM? Assessing gender differentials in the factors associated with transition to first jobs

PubMed Central

Sassler, Sharon; Glass, Jennifer; Levitte, Yael; Michelmore, Katherine M.

2016-01-01

Women remain underrepresented in the STEM workforce. We assess explanations for women's underrepresentation in STEM jobs, focusing on a cohort that came of age in the 1980s and 1990s, when women dramatically increased their representation in the scientific labor force. Data are from the NLSY79, and our analysis focuses on members of this cohort who received a college degree, with an emphasis on those who completed a degree in a STEM field. Our analyses test the extent to which college major, expectations to work in STEM, and family expectations shaped transitions into STEM occupations within two years of degree completion. Among those majoring in STEM fields there were no gender differences in transitioning into STEM jobs, though there were sizable differences in transitions to STEM employment by field of study. Of note are gender differences in associations between family expectations and transitions into STEM employment. The most career oriented women, who expected to marry late and limit fertility, were no more likely to enter STEM jobs than were women who anticipated marrying young and having two or more children. The men most likely to enter STEM occupations, in contrast, adhered to significantly more conventional gender ideologies than their female counterparts, expecting to marry at younger ages but also to remain childless. Results of our regression decomposition indicated that marriage and family expectations and gender ideology worked in opposite directions for men and women. Nonetheless, the majority of the gender disparity in transitions into STEM jobs was related to women's underrepresentation in engineering and computer science fields of study. PMID:28202143

What’s So Special about STEM? A Comparison of Women’s Retention in STEM and Professional Occupations

PubMed Central

Sassler, Sharon; Levitte, Yael; Michelmore, Katherine M.

2014-01-01

We follow female college graduates in the National Longitudinal Survey of Youth 1979 and compare the trajectories of women in science, technology, engineering, and mathematics (STEM)-related occupations to other professional occupations. Results show that women in STEM occupations are significantly more likely to leave their occupational field than professional women, especially early in their career, while few women in either group leave jobs to exit the labor force. Family factors cannot account for the differential loss of STEM workers compared to other professional workers. Few differences in job characteristics emerge either, so these cannot account for the disproportionate loss of STEM workers. What does emerge is that investments and job rewards that generally stimulate field commitment, such as advanced training and high job satisfaction, fail to build commitment among women in STEM. PMID:25554713

Weeded Out? Gendered Responses to Failing Calculus.

PubMed

Sanabria, Tanya; Penner, Andrew

2017-06-01

Although women graduate from college at higher rates than men, they remain underrepresented in science, technology, engineering, and mathematics (STEM) fields. This study examines whether women react to failing a STEM weed-out course by switching to a non-STEM major and graduating with a bachelor's degree in a non-STEM field. While competitive courses designed to weed out potential STEM majors are often invoked in discussions around why students exit the STEM pipeline, relatively little is known about how women and men react to failing these courses. We use detailed individual-level data from the National Educational Longitudinal Study (NELS) Postsecondary Transcript Study (PETS): 1988-2000 to show that women who failed an introductory calculus course are substantially less likely to earn a bachelor's degree in STEM. In doing so, we provide evidence that weed-out course failure might help us to better understand why women are less likely to earn degrees.

Weeded Out? Gendered Responses to Failing Calculus

PubMed Central

Sanabria, Tanya; Penner, Andrew

2018-01-01

Although women graduate from college at higher rates than men, they remain underrepresented in science, technology, engineering, and mathematics (STEM) fields. This study examines whether women react to failing a STEM weed-out course by switching to a non-STEM major and graduating with a bachelor’s degree in a non-STEM field. While competitive courses designed to weed out potential STEM majors are often invoked in discussions around why students exit the STEM pipeline, relatively little is known about how women and men react to failing these courses. We use detailed individual-level data from the National Educational Longitudinal Study (NELS) Postsecondary Transcript Study (PETS): 1988–2000 to show that women who failed an introductory calculus course are substantially less likely to earn a bachelor’s degree in STEM. In doing so, we provide evidence that weed-out course failure might help us to better understand why women are less likely to earn degrees. PMID:29616148

A Nuanced Look at Women in STEM Fields at Two-Year Colleges: Factors That Shape Female Students' Transfer Intent.

PubMed

Wang, Xueli; Chan, Hsun-Yu; Soffa, Sara Jimenez; Nachman, Brett Ranon

2017-01-01

In this study, we explored the relationship between the intent to transfer upward and a set of motivational, contextual, and socio-demographic background factors among 696 female students beginning in science, technology, engineering, and mathematics (STEM) programs or courses at two-year colleges in a Midwestern state. Drawing upon survey data and administrative records, our multinomial logistic regression analysis revealed that students' math and science self-efficacy beliefs, as well as transfer-oriented interaction, were significant and positive predictors for their intent to transfer into STEM fields as opposed to having no intent to transfer. In addition, the association between transfer intent and these key motivational and contextual factors was moderated by students' racial/ethnic backgrounds, marital status, and childcare obligations. For example, despite the positive relationship between transfer-oriented interaction and the intention to transfer into STEM fields, Black women were less likely to have intent to transfer into STEM fields than White students until Black students reported a moderate level of transfer-oriented interaction. Conversely, Hispanic students were more likely to report intent to transfer into STEM fields than their White peers, even when Hispanic students reported a relatively low level of engagement in transfer-oriented interaction. These and other reported findings bear important and nuanced implications as policymakers, educators, and researchers continue to discover ways to better support women's educational pathways and success in STEM fields at and through two-year colleges.

A Nuanced Look at Women in STEM Fields at Two-Year Colleges: Factors That Shape Female Students' Transfer Intent

PubMed Central

Wang, Xueli; Chan, Hsun-yu; Soffa, Sara Jimenez; Nachman, Brett Ranon

2017-01-01

In this study, we explored the relationship between the intent to transfer upward and a set of motivational, contextual, and socio-demographic background factors among 696 female students beginning in science, technology, engineering, and mathematics (STEM) programs or courses at two-year colleges in a Midwestern state. Drawing upon survey data and administrative records, our multinomial logistic regression analysis revealed that students' math and science self-efficacy beliefs, as well as transfer-oriented interaction, were significant and positive predictors for their intent to transfer into STEM fields as opposed to having no intent to transfer. In addition, the association between transfer intent and these key motivational and contextual factors was moderated by students' racial/ethnic backgrounds, marital status, and childcare obligations. For example, despite the positive relationship between transfer-oriented interaction and the intention to transfer into STEM fields, Black women were less likely to have intent to transfer into STEM fields than White students until Black students reported a moderate level of transfer-oriented interaction. Conversely, Hispanic students were more likely to report intent to transfer into STEM fields than their White peers, even when Hispanic students reported a relatively low level of engagement in transfer-oriented interaction. These and other reported findings bear important and nuanced implications as policymakers, educators, and researchers continue to discover ways to better support women's educational pathways and success in STEM fields at and through two-year colleges. PMID:28220102

Report on STEM Graduation and Enrollment Trends. April 2013

ERIC Educational Resources Information Center

Jenkins, Rick; Butler, Sharon; Mitchell, Suzanne

2013-01-01

The purpose of this report on Arkansas STEM (Science, Technology, Engineering, and Mathematics) program activity is to inform education and policy makers about the need to prepare and graduate more students with degrees in STEM-related fields as defined by the U.S. Immigration and Customs Enforcement (ICE). Arkansas is witnessing a significant…

Near-Peer Mentor Model: Synergy within Mentoring

ERIC Educational Resources Information Center

Anderson, Margery K.; Tenenbaum, Laura S.; Ramadorai, Swati B.; Yourick, Debra L.

2015-01-01

The near-peer mentor model provides undergraduates and recent post-baccalaureates in the science, technology, engineering, and mathematics (STEM) fields with an internship in two related disciplines, STEM research and STEM education. The near-peer mentor is both a mentored research intern and a mentor to pre-college students. During the 2013…

Report on STEM Graduation and Enrollment Trends. February 2014

ERIC Educational Resources Information Center

Jenkins, Rick; Butler, Sharon; Mitchell, Suzanne

2014-01-01

The purpose of this report on Arkansas STEM (Science, Technology, Engineering, and Mathematics) program activity is to inform education and policy makers about the need to prepare and graduate more students with degrees in STEM related fields as defined by the U.S. Immigration and Customs Enforcement (ICE). Arkansas is witnessing a significant…

Report on STEM Graduation and Enrollment Trends. June 2012

ERIC Educational Resources Information Center

Jenkins, Rick; Mitchell, Suzanne

2012-01-01

The purpose of this report on Arkansas STEM (Science, Technology, Engineering, and Mathematics) program activity is to inform education and policy makers about the need to prepare and graduate more students with degrees in STEM related fields as defined by the U.S. Immigration and Customs Enforcement (ICE). Arkansas is witnessing a significant…

Report on STEM Graduation and Enrollment Trends. January 2015

ERIC Educational Resources Information Center

Jenkins, Rick; Butler, Sharon; Mitchell, Suzanne

2015-01-01

The purpose of this report on Arkansas STEM (Science, Technology, Engineering, and Mathematics) program activity is to inform education and policy makers about the need to prepare and graduate more students with degrees in STEM related fields as defined by the U.S. Immigration and Customs Enforcement (ICE). Arkansas is witnessing a significant…

Role Models and Informal STEM-Related Activities Positively Impact Female Interest in STEM

ERIC Educational Resources Information Center

Weber, Katherine

2011-01-01

The United States' economy depends greatly on a citizenry that possesses scientific and technical skills within the fields of science, technology, engineering, and mathematics (STEM) for economic growth. In the past few decades, technological advancement has created a demand for a highly skilled workforce possessing scientific and mathematical…

Supply and Demand of STEM Workers: STEM Jobs Are Growing, but Are Enough Massachusetts Students Qualified? Education Research Brief. Issue 2

ERIC Educational Resources Information Center

Conaway, Carrie

2007-01-01

Jobs in science, technology, engineering, and mathematics (STEM) are the backbone of the Massachusetts economy. They comprise a substantial share of employment: about 13 percent of the state's jobs and one-third of its gross state product are related to STEM. And they also generate jobs in other fields, such as business and professional services,…

Ecocritically (Re)Considering STEM Integrated Ecological Inquiry in Teacher Education

ERIC Educational Resources Information Center

Lupinacci, John; Happel-Parkins, Alison

2017-01-01

The acronym STEM is a ubiquitous term for seemingly anything in--or related to--the fields of science, technology, engineering, and mathematics, and the current dominant educational STEM discourse in teacher education is often organized around questions of how to integrate math and science into the other content areas or vice versa. The purpose of…

Modeling the Relation between Students' Implicit Beliefs about Their Abilities and Their Educational STEM Choices

ERIC Educational Resources Information Center

van Aalderen-Smeets, Sandra I.; Walma van der Molen, Juliette H.

2018-01-01

Despite the large body of research on students' educational and career choices in the field of technology, design, and science, we still lack a clear understanding of how to stimulate more students to opt for a study path or career within the STEM fields (Science, Technology, Engineering, Mathematics). In this article, we outline a new theoretical…

When trying hard isn't natural: women's belonging with and motivation for male-dominated STEM fields as a function of effort expenditure concerns.

PubMed

Smith, Jessi L; Lewis, Karyn L; Hawthorne, Lauren; Hodges, Sara D

2013-02-01

Feeling like one exerts more effort than others may influence women's feelings of belonging with science, technology, engineering, and math (STEM) and impede their motivation. In Study 1, women STEM graduate students perceived they exerted more effort than peers to succeed. For women, but not men, this effort expenditure perception predicted a decreased sense of belonging, which in turn decreased motivation. Study 2 tested whether the male-dominated status of a field triggers such effort expectations. We created a fictional "eco-psychology" graduate program, which when depicted as male-dominated resulted in women expecting to exert relatively more effort and decreased their interest in pursuing the field. Study 3 found emphasizing effort as expected (and normal) to achieve success elevated women's feelings of belonging and future motivation. Results suggest effort expenditure perceptions are an indicator women use to assess their fit in STEM. Implications for enhancing women's participation in STEM are discussed.

Effect of Out-of-School Time STEM Education Programs: Implications for Policy

NASA Astrophysics Data System (ADS)

Talbot, Harry A.

Today's world requires greater STEM knowledge for employment and understanding of emerging issues. A predicted 3 million jobs will be created in STEM-related fields but the percentage of earned STEM-related degrees is diminishing. A lack of progress in STEM education for American students is most pronounced among females who make up 48% of the workforce and 24% of STEM employees. A lack of STEM interest among students is compounded by limited time in the school day for STEM topics, lack of teacher confidence in teaching STEM, and a lack of professional development. This study examines the impact of Out-of-School-Time (OST) programs on knowledge acquisition and attitudes toward STEM topics by gender. Program content was delivered by undergraduate pre-teacher candidates and undergraduate STEM majors, using a structured, hands-on engineering program developed for the National Aeronautics and Space Administration (NASA). Monthly professional development was provided to OST staff by NASA content specialists and instructors from Fresno State University. A repeated-measures design analyzed group differences across three points in time: prior to the start of instruction (pretest), immediately following the end of instruction (posttest), and 60 days following (post posttest). A within-group comparison measured posttest and post-post-test changes for each gender. Program students included in the study participated for at least 12 of the 24 program hours offered and completed all three assessments. The findings showed that STEM knowledge acquisition advanced at similar levels for both genders. These results were consistent with the existing research. Findings related to attitudes toward STEM topics showed that female students did not change over time but males students' interest lessened over time. These findings did not support the current research in this area. Recommendations for practice include developing programs that focus on gender differentiated learning styles, linking pre-service teachers with undergraduate STEM majors in the delivery of OST STEM content and skill development, and creating an environment that links the regular day school programs, OST programs, family, media,and cultural institutions to support STEM education. Universities should also play a leading role in the training of future teachers and STEM-field practitioners.

Why are some STEM fields more gender balanced than others?

PubMed

Cheryan, Sapna; Ziegler, Sianna A; Montoya, Amanda K; Jiang, Lily

2017-01-01

Women obtain more than half of U.S. undergraduate degrees in biology, chemistry, and mathematics, yet they earn less than 20% of computer science, engineering, and physics undergraduate degrees (National Science Foundation, 2014a). Gender differences in interest in computer science, engineering, and physics appear even before college. Why are women represented in some science, technology, engineering, and mathematics (STEM) fields more than others? We conduct a critical review of the most commonly cited factors explaining gender disparities in STEM participation and investigate whether these factors explain differential gender participation across STEM fields. Math performance and discrimination influence who enters STEM, but there is little evidence to date that these factors explain why women's underrepresentation is relatively worse in some STEM fields. We introduce a model with three overarching factors to explain the larger gender gaps in participation in computer science, engineering, and physics than in biology, chemistry, and mathematics: (a) masculine cultures that signal a lower sense of belonging to women than men, (b) a lack of sufficient early experience with computer science, engineering, and physics, and (c) gender gaps in self-efficacy. Efforts to increase women's participation in computer science, engineering, and physics may benefit from changing masculine cultures and providing students with early experiences that signal equally to both girls and boys that they belong and can succeed in these fields. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

Alternate Reality Games as an Informal Learning Tool for Generating STEM Engagement among Underrepresented Youth: A Qualitative Evaluation of the Source

ERIC Educational Resources Information Center

Gilliam, Melissa; Jagoda, Patrick; Fabiyi, Camille; Lyman, Phoebe; Wilson, Claire; Hill, Brandon; Bouris, Alida

2017-01-01

This project developed and studied "The Source," an alternate reality game (ARG) designed to foster interest and knowledge related to science, technology, engineering, and math (STEM) among youth from populations underrepresented in STEM fields. ARGs are multiplayer games that engage participants across several media such as shared…

A Qualitative Investigation of African Americans' Decision to Pursue Computing Science Degrees: Implications for Cultivating Career Choice and Aspiration

ERIC Educational Resources Information Center

Charleston, LaVar J.

2012-01-01

According to Pearson (2002), minority groups are not well represented in science, technology, engineering, and mathematics (STEM) occupations. Among these underrepresented groups are African Americans. To ensure the economic vitality of the STEM workforce in the United States, it is imperative to broaden participation in STEM-related fields and…

Stem cells in retinal regeneration: past, present and future.

PubMed

Ramsden, Conor M; Powner, Michael B; Carr, Amanda-Jayne F; Smart, Matthew J K; da Cruz, Lyndon; Coffey, Peter J

2013-06-01

Stem cell therapy for retinal disease is under way, and several clinical trials are currently recruiting. These trials use human embryonic, foetal and umbilical cord tissue-derived stem cells and bone marrow-derived stem cells to treat visual disorders such as age-related macular degeneration, Stargardt's disease and retinitis pigmentosa. Over a decade of analysing the developmental cues involved in retinal generation and stem cell biology, coupled with extensive surgical research, have yielded differing cellular approaches to tackle these retinopathies. Here, we review these various stem cell-based approaches for treating retinal diseases and discuss future directions and challenges for the field.

The effect of video interviews with STEM professionals on STEM-subject attitude and STEM-career interest of middle school students in conservative Protestant Christian schools

NASA Astrophysics Data System (ADS)

Alsup, Philip R.

Inspiring learners toward career options available in STEM fields (Science, Technology, Engineering, and Mathematics) is important not only for economic development but also for maintaining creative thinking and innovation. Limited amounts of research in STEM education have focused on the population of students enrolled in religious and parochial schools, and given the historic conflict between religion and science, this sector of American education is worthy of examination. The purpose of this quantitative study is to extend Gottfredson's (1981) Theory of Circumscription and Compromise as it relates to occupational aspirations. Bem's (1981) Gender Schema Theory is examined as it relates to the role of gender in career expectations, and Crenshaw's (1989) Intersectionality Theory is included as it pertains to religion as a group identifier. Six professionals in STEM career fields were video recorded while being interviewed about their skills and education as well as positive and negative aspects of their jobs. The interviews were compiled into a 25-minute video for the purpose of increasing understanding of STEM careers among middle school viewers. The research questions asked whether middle school students from conservative, Protestant Christian schools in a Midwest region increased in STEM-subject attitude and STEM-career interest as a result of viewing the video and whether gender interacted with exposure to the video. A quasi-experimental, nonequivalent control groups, pretest/posttest factorial design was employed to evaluate data collected from the STEM Semantic Survey. A Two-Way ANCOVA revealed no significant differences in dependent variables from pretest to posttest. Implications of the findings are examined and recommendations for future research are made. Descriptors: STEM career interest, STEM attitude, STEM gender disparity, Occupational aspirations, Conservative Protestant education.

Electric field imaging of single atoms

PubMed Central

Shibata, Naoya; Seki, Takehito; Sánchez-Santolino, Gabriel; Findlay, Scott D.; Kohno, Yuji; Matsumoto, Takao; Ishikawa, Ryo; Ikuhara, Yuichi

2017-01-01

In scanning transmission electron microscopy (STEM), single atoms can be imaged by detecting electrons scattered through high angles using post-specimen, annular-type detectors. Recently, it has been shown that the atomic-scale electric field of both the positive atomic nuclei and the surrounding negative electrons within crystalline materials can be probed by atomic-resolution differential phase contrast STEM. Here we demonstrate the real-space imaging of the (projected) atomic electric field distribution inside single Au atoms, using sub-Å spatial resolution STEM combined with a high-speed segmented detector. We directly visualize that the electric field distribution (blurred by the sub-Å size electron probe) drastically changes within the single Au atom in a shape that relates to the spatial variation of total charge density within the atom. Atomic-resolution electric field mapping with single-atom sensitivity enables us to examine their detailed internal and boundary structures. PMID:28555629

STEM-related, Student-led Service Learning / Community Engagement Projects: Examples and Benefits

NASA Astrophysics Data System (ADS)

Swap, R. J.; Wayland, K.

2015-12-01

Field-based, STEM-related service learning / community engagement projects present an opportunity for undergraduate students to demonstrate proficiencies related to the process of inquiry. These proficiencies include: appreciation of the larger project context, articulation of an informed question/hypothesis, project proposal development, interdisciplinary collaboration, project management (including planning, implementation reconfiguration and synthesis) and lastly the generation and handing off of acquired knowledge. Calls for these types of proficiencies have been expressed by governmental, non-governmental as well as the private sector. Accordingly, institutions of higher learning have viewed such activities as opportunities for enriching the learning experience for undergraduate students and for making such students more marketable, especially those from STEM-related fields. This institutional interest has provided an opportunity to support and expand field-based learning. Here we present examples of student-led/faculty-mentored international service learning and community engagement projects along the arc of preparation, implementation and post-field process. Representative examples that draw upon environmental science and engineering knowledge have been selected from more than 20 international undergraduate student projects over past decade and include: slow-sand water filtration, rainwater harvesting, methane biodigesters, water reticulation schemes and development and implementation of rocket stoves for communal cooking. We discuss these efforts in terms of the development of the aforementioned proficiencies, the utility of such proficiencies to the larger enterprise of STEM and the potential for transformative student learning outcomes. We share these experiences and lessons learned with the hope that others may intelligently borrow from our approach in a manner appropriate for their particular context.

Perceptions of Self-Efficacy among STEM Students with Disabilities

ERIC Educational Resources Information Center

Jenson, Ronda J.; Petri, Alexis N.; Day, Arden D.; Truman, Kevin Z.; Duffy, Kate

2011-01-01

Numerous studies examine the relationship between self-efficacy and positive outcomes for postsecondary students. Collectively they echo that self-efficacy is an essential component to positive outcomes. Relatively few studies focused on students with disabilities majoring in STEM fields. Twenty postsecondary students with disabilities…

Succeeding as a Student in the STEM Fields with an Invisible Disability: A College Handbook for Science, Technology, Engineering, and Math Students with Autism, ADD, Affective Disorders, or Learning Diffculties and Their Families

ERIC Educational Resources Information Center

Oslund, Christy

2013-01-01

The STEM fields (Science, Technology, Engineering and Math) attract many students with autism, ADD, affective disorders and related invisible disabilities who are highly intelligent and analytical, but who, upon entering higher education, may find that they struggle with independent living and a different way of learning. This is a preparation…

STEM Field Persistence: The Impact of Engagement on Postsecondary STEM Persistence for Underrepresented Minority Students

ERIC Educational Resources Information Center

Flynn, Daniel T.

2016-01-01

Persistence studies in science, technology, engineering, and math (STEM) fields indicate that the pipeline to degree attainment is "leaky" and underrepresented minorities are not persisting in the STEM fields. Those students who do not persist in the STEM fields either migrate to other fields of study or drop out of higher education…

Motivational decline and recovery in higher education STEM courses

NASA Astrophysics Data System (ADS)

Young, Anna M.; Wendel, Paul J.; Esson, Joan M.; Plank, Kathryn M.

2018-06-01

Decline in student motivation is a concern for STEM education, especially for underrepresented groups in the sciences. Using the Science Motivation Questionnaire II, 41 foundational STEM courses were surveyed at the beginning and end of each semester in an academic year at a small primarily undergraduate university. Significant pre- to post-semester declines were observed in each of five measured motivational factors (Intrinsic motivation, Career motivation, Self determination, Self-efficacy, and Grade motivation), with effect sizes ranging from 0.21 to 0.41. However, in the second semester pre-survey, four motivational factors rebounded, including three returning to initial levels, suggesting that the observed motivational decline is not long-lasting. Analysis suggests that declines are not related to survey fatigue or student demographics, but rather to grades and, in the case of one motivational factor, to academic field. These findings suggest that a refocus on grading practices across STEM fields may influence student motivation and persistence in STEM.

Cognitive and Motivational Factors that Inspire Hispanic Female Students to Pursue STEM-Related Academic Programs that Lead to Careers in Science, Technology, Engineering, and Mathematics

NASA Astrophysics Data System (ADS)

Morel-Baker, Sonaliz

Hispanics, and women in particular, continue to be underrepresented in the fields of science, technology, engineering, and mathematics (STEM). The purpose of this study was to analyze cognitive and motivational factors that inspired Hispanic female college students to major in STEM programs and aspire to academic success. This mixed methods study was conducted using both quantitative and qualitative data collection and analysis techniques in a sequential phase. Quantitative data were collected through the use of the 80-item Honey and Mumford Learning Styles Questionnaire, which was focused on the students' learning styles and how they impact Hispanic female students upon engaging in a STEM-related curriculum. Qualitative data were collected during interviews focusing on factors that led students to select, participate in, and make a commitment to some aspect of a STEM-related program. The questions that were asked during the interviews were intended to examine whether the existence of role models and STEM initiatives motivate Hispanic female students to major in STEM-related academic programs and aspire to academic success. The participants in this study were undergraduate Hispanic female students majoring in STEM-related academic programs and at a four-year university. The results indicate that the majority of the participants (88%) identified as reflectors, 4% as activists, 4% as theorists, and 4% as pragmatists. The results from the interviews suggested that the existence of role models (family members, educators, or STEM professionals) was a factor that motivated Hispanic females to major in STEM-related subjects and that exposure to STEM initiatives during K-12 education motivated Hispanic females to pursue a career in STEM.

The Single Sex Debate for Girls in Science: a Comparison Between Two Informal Science Programs on Middle School Students' STEM Identity Formation

NASA Astrophysics Data System (ADS)

Hughes, Roxanne M.; Nzekwe, Brandon; Molyneaux, Kristen J.

2013-10-01

Currently, there are policy debates regarding the efficacy and legality of single sex formal and informal education programs. This issue is particularly poignant in science education due to the historical marginalization of women in these fields. This marginalization has resulted in women being positioned as a stigmatized group within many science, technology, engineering, and mathematics (STEM) related fields. Research points to adolescence as the age where this sense of marginalization begins to develop. As a result, policy responses have utilized various frameworks such as: increased access for women, changing pedagogy to address women's learning styles, changing the language and culture of science to prevent marginalization of stigmatized groups, and finally exploring the role that individual identity plays in the marginalization of women. This study adds to the policy debate as it applies to single sex education by comparing middle school participants' STEM identity formation during two informal science learning environments (an all girls' STEM camp and a co-educational STEM camp). Additionally, this study focuses on the influence of camp activities within two informal science education programs: particularly the provision of role models and authentic STEM research activities, as means to improve STEM identity and make these fields relevant to the lives of middle school students. The results indicate that both camps improved girls' STEM identities. These findings suggest that the single sex environment is not as important to STEM identity as the pedagogy used within the program.

The identification of Gender Bias in the U.S. Military

DTIC Science & Technology

2018-03-01

Opinion Form SSA Social Security Administration STEM Science, Technology, Engineering, and Math U.S. United States USN United States Navy xvi THIS...in science, technology, engineering, and math (STEM) related fields, with lifestyle choice being cited by some as the reasoning for this disparity

[Research with human embryo stem cells. Foundations and judicial limits].

PubMed

Eser, Albin; Koch, Hans-Georg

2004-01-01

Research with human embryos, and particularly, the use for scientific purposes of human embryonic stem cells has given raise to different sort of problems at the international level. One of the most strict regulation in this field, is this lecture Professors Albin Eser and Hans-Georg Koch analyse the german legal framework in relation with the use of embryos and human embryonic stem cells for scientific purposes.

Cognitive and Motivational Factors That Inspire Hispanic Female Students to Pursue STEM-Related Academic Programs That Lead to Careers in Science, Technology, Engineering, and Mathematics

ERIC Educational Resources Information Center

Morel-Baker, Sonaliz

2017-01-01

Hispanics, and women in particular, continue to be underrepresented in the fields of science, technology, engineering, and mathematics (STEM). The purpose of this study was to analyze cognitive and motivational factors that inspired Hispanic female college students to major in STEM programs and aspire to academic success. This mixed methods study…

Self-efficacy of college freshmen engaged in STEM outreach

NASA Astrophysics Data System (ADS)

Patchin, Stephen H.

Not since the Cold War and the launch of Sputnik has there been such a focus on producing college graduates in fields related to science, technology, engineering, and mathematics (STEM). As manually driven careers disappear, new diverse careers are created and they have one thing in common, STEM. As students move into these challenging curriculums they will need to have faith in their abilities to achieve their goals. This self-efficacy is vital component for their collegiate and career success. This mixed methods study examines the unique pre-college STEM outreach phenomenon called Mind Trekkers. Mind Trekkers uses the "WOW" of experiential learning in the areas of STEM to motivate K-12 students to engage in STEM related fields. The focus of the study is on the first-year college freshmen that join this program, becoming STEM serviceteers, and how being part of this STEM phenomenon impacts their self-efficacy. The findings can be summed up in a quote. I get to help people understanding in a different way than I would if I was just doing volunteering like I did in high school. It's cool. I just love it and it gives me the confidence that what I am doing is the right thing here at (the university). (Jean). The results of the study indicate that the Mind Trekkers program acted as a catalyst to increase the self-efficacy of the students that participated in it, through personal social and academic impact.

Predicting Undergraduates' Persistence in Science, Technology, Engineering, and Math Fields

NASA Astrophysics Data System (ADS)

Koch, Amanda Joy

A national shortage of workers in Science, Technology, Engineering, and Math (STEM) occupations has led to efforts to identify why people leave these fields. Lower persistence rates in STEM for females than for males have also led to examinations of features that cause females to leave STEM fields. The current study examines individual- and school-level features that influence undergraduate students' decisions to leave STEM majors, focusing on potential explanations for why females are more likely than males to leave. Persistence in STEM was examined in three samples: (a) persistence through the second year of college in a sample of high school seniors interested in STEM majors; (b) persistence through the fourth year of college in a sample of second year undergraduate STEM majors; and (c) persistence through the second, third, and fourth years of college in a sample of high school seniors interested in STEM majors. Differences between persistence in male-dominated and non-male-dominated STEM majors were also examined. In all samples, gender differences were found for most individual-level predictors, with males tending to score higher than females on measures such as SAT-Math, self-rated STEM ability, and high school extracurricular activities and awards in STEM. On the other hand, females earned better high school grades and had stronger relative non-STEM ability and achievement than males. Bivariate analyses indicated that those who persisted in STEM majors typically had higher scores than those who did not persist for SAT-Math, high school achievement, STEM course taking, undergraduate STEM grades, self-rated STEM ability, interest in STEM, extracurricular activities and awards in STEM, degree goals, and socioeconomic status. Multivariate analyses identified SAT-Math as one of the best predictors of persistence in high school samples, and undergraduate STEM GPA was one of the best predictors in the samples of second year undergraduates. In several samples, a significant cross-level interaction was found between gender and undergraduate females' college-level proportional representation in STEM; however, the effects were inconsistent across samples. Even when controlling for various individual- and school-level predictors, gender effects tended to remain significant, with females in most samples leaving STEM majors at higher rates than males.

Cancer stem cell research in Iran: potentials and challenges.

PubMed

Roudi, Raheleh; Ebrahimi, Marzieh; Shariftabrizi, Ahmad; Madjd, Zahra

2017-08-01

Treatment modalities can reduce cancer-related mortality; however, a majority of patients develop drug resistance, metastasis and relapse. It has been proposed that tumorigenic characteristics of tumors are related to a proportion of cancer cells, termed cancer stem cells (CSCs). Following the first evidence regarding the existence of CSC population in acute myeloid leukemia in 1997, publications in CSCs field showed an explosive trend in all cancer types around the world. First research paper in the field of CSCs in Iran was published in 2004 on prostate cancer. Subsequently, an annual number of publications in the field of CSCs displayed a rapidly growing trend. Therefore, in the current review, we have presented a comprehensive evaluation of the CSCs research in Iran.

Toward a Framework for Multicultural STEM-Focused Career Interventions.

PubMed

Byars-Winston, Angela

2014-12-14

Numerous federal and national commissions have called for policies, funds, and initiatives aimed at expanding the nation's science, technology, engineering, and mathematics (STEM) workforce and education investments to create a significantly larger, more diverse talent pool of individuals who pursue technical careers. Career development professionals are poised to contribute to the equity discourse about broadening STEM participation. However, few are aware of STEM-related career development matters, career opportunities and pathways, or strategies for promoting STEM pursuits. The author summarizes STEM education and workforce trends and articulates an equity imperative for broadening and diversifying STEM participation. The author then offers a multicultural STEM-focused career development framework to encourage career development professionals' knowledge and awareness of STEM education and careers and delineates considerations for practice aimed at increasing the attainment and achievement of diverse groups in STEM fields.

Toward a Framework for Multicultural STEM-Focused Career Interventions

PubMed Central

Byars-Winston, Angela

2015-01-01

Numerous federal and national commissions have called for policies, funds, and initiatives aimed at expanding the nation's science, technology, engineering, and mathematics (STEM) workforce and education investments to create a significantly larger, more diverse talent pool of individuals who pursue technical careers. Career development professionals are poised to contribute to the equity discourse about broadening STEM participation. However, few are aware of STEM-related career development matters, career opportunities and pathways, or strategies for promoting STEM pursuits. The author summarizes STEM education and workforce trends and articulates an equity imperative for broadening and diversifying STEM participation. The author then offers a multicultural STEM-focused career development framework to encourage career development professionals' knowledge and awareness of STEM education and careers and delineates considerations for practice aimed at increasing the attainment and achievement of diverse groups in STEM fields. PMID:25750480

Preparing Students for Middle School Through After-School STEM Activities

NASA Astrophysics Data System (ADS)

Moreno, Nancy P.; Tharp, Barbara Z.; Vogt, Gregory; Newell, Alana D.; Burnett, Christopher A.

2016-12-01

The middle school years are a crucial time for cultivating students' interest in and preparedness for future STEM careers. However, not all middle school children are provided opportunities to engage, learn and achieve in STEM subject areas. Engineering, in particular, is neglected in these grades because it usually is not part of science or mathematics curricula. This study investigates the effectiveness of an engineering-integrated STEM curriculum designed for use in an after-school environment. The inquiry-based activities comprising the unit, Think Like an Astronaut, were intended to introduce students to STEM careers—specifically engineering and aerospace engineering—and enhance their skills and knowledge applicable related to typical middle school science objectives. Results of a field test with a diverse population of 5th grade students in nine schools revealed that Think Like an Astronaut lessons are appropriate for an after-school environment, and may potentially help increase students' STEM-related content knowledge and skills.

Perceived Mathematical Ability under Challenge: A Longitudinal Perspective on Sex Segregation among STEM Degree Fields

ERIC Educational Resources Information Center

Nix, Samantha; Perez-Felkner, Lara; Thomas, Kirby

2015-01-01

Students' perceptions of their mathematics ability vary by gender and seem to influence science, technology, engineering, and mathematics (STEM) degree choice. Related, students' perceptions during academic difficulty are increasingly studied in educational psychology, suggesting a link between such perceptions and task persistence. Despite…

Preparing STEM Teachers: The Key to Global Competitiveness

ERIC Educational Resources Information Center

American Association of Colleges for Teacher Education (NJ1), 2007

2007-01-01

Like many sectors in society, the teacher preparation community is working to address challenges the nation faces in relation to global competitiveness. This document highlights institutions around the country that are preparing tomorrow's teachers in the fields of science, technology, engineering, and mathematics (STEM). Well-known shortages of…

Evaluation of alternative planting strategies to reduce wheat stem sawfly (Hymenoptera: Cephidae) damage to spring wheat in the northern Great Plains.

PubMed

Beres, B L; Cárcamo, H A; Bremer, E

2009-12-01

Wheat, Triticum aestivum L., producers are often reluctant to use solid-stemmed wheat cultivars resistant to wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), due to concerns regarding yield, efficacy or market opportunities. We evaluated the impact of several planting strategies on wheat yield and quality and wheat stem sawfly infestation at two locations over a three-year period. Experimental units consisted of large plots (50 by 200 m) located on commercial farms adjacent to wheat stem sawfly-infested fields. Compared with a monoculture of a hollow-stemmed cultivar ('AC Barrie'), planting a monoculture of a solid-stemmed cultivar ('AC Eatonia') increased yield by an average of 16% (0.4 mg ha(-1)) and increased the grade of wheat by one unit at the two most heavily infested site-years. Planting a 1:1 blend of AC Eatonia and AC Barrie increased yield by an average of 11%, whereas planting 20- or 40-m plot margins to AC Eatonia increased yield by an average of 8%. High wheat stem sawfly pressure limited the effectiveness of using resistant cultivars in field margins because plants were often infested beyond the plot margin, with uniform infestation down the length of the plots at the two most heavily infested site-years. The effectiveness of AC Eatonia to reduce wheat stem sawfly survivorship was modest in this study, probably due to weather-related factors influencing pith expression and to the high abundance of wheat stem sawfly. Greater benefits from planting field margins to resistant cultivars or planting a blend of resistant and susceptible cultivars might be achievable under lower wheat stem sawfly pressure.

Malleability in communal goals and beliefs influences attraction to stem careers: evidence for a goal congruity perspective.

PubMed

Diekman, Amanda B; Clark, Emily K; Johnston, Amanda M; Brown, Elizabeth R; Steinberg, Mia

2011-11-01

The goal congruity perspective posits that 2 distinct social cognitions predict attraction to science, technology, engineering, or mathematics (STEM) fields. First, individuals may particularly value communal goals (e.g., working with or helping others), due to either chronic individual differences or the salience of these goals in particular contexts. Second, individuals hold beliefs about the activities that facilitate or impede these goals, or goal affordance stereotypes. Women's tendency to endorse communal goals more highly than do men, along with consensual stereotypes that STEM careers impede communal goals, intersect to produce disinterest in STEM careers. We provide evidence for the foundational predictions that gender differences emerge primarily on communal rather than agentic goals (Studies 1a and 3) and that goal affordance stereotypes reflect beliefs that STEM careers are relatively dissociated from communal goals (Studies 1b and 1c). Most critically, we provide causal evidence that activated communal goals decrease interest in STEM fields (Study 2) and that the potential for a STEM career to afford communal goals elicits greater positivity (Study 3). These studies thus provide a novel demonstration that understanding communal goals and goal affordance stereotypes can lend insight into attitudes toward STEM pursuits.

Pressurizing the STEM Pipeline: an Expectancy-Value Theory Analysis of Youths' STEM Attitudes

NASA Astrophysics Data System (ADS)

Ball, Christopher; Huang, Kuo-Ting; Cotten, Shelia R.; Rikard, R. V.

2017-08-01

Over the past decade, there has been a strong national push to increase minority students' positive attitudes towards STEM-related careers. However, despite this focus, minority students have remained underrepresented in these fields. Some researchers have directed their attention towards improving the STEM pipeline which carries students through our educational system and into STEM careers. Previous research has shown that expectancy-value theory (EVT) is useful for examining the short-term as well as long-term academic motivations and intentions of elementary age minority students. These findings provide insights into ways we may be able to potentially "patch" particular STEM pipeline leaks. In the current study, we advance this research by using EVT as a framework to examine the STEM attitudes of young students directly. We hypothesize that students' academic-related expectancies for success and subjective task values will be associated with an increase in STEM attitudes. Data for this study was gathered over the course of a large-scale computing intervention which sought to increase students' STEM interest. This computing intervention took place in an urban elementary school district located within the southeastern USA. Results from this study indicate that both intrinsic values and utility values predict students' STEM attitudes but they influence attitudes related to the various dimensions of STEM differently. These findings demonstrate that EVT provides a useful framework, which can be integrated into future computing interventions, to help encourage positive STEM attitudes in young children, thus increasing the internal pressure (or flow) within the STEM pipeline.

Nanotechnology and stem cell therapy for cardiovascular diseases: potential applications.

PubMed

La Francesca, Saverio

2012-01-01

The use of stem cell therapy for the treatment of cardiovascular diseases has generated significant interest in recent years. Limitations to the clinical application of this therapy center on issues of stem cell delivery, engraftment, and fate. Nanotechnology-based cell labeling and imaging techniques facilitate stem cell tracking and engraftment studies. Nanotechnology also brings exciting new opportunities to translational stem cell research as it enables the controlled engineering of nanoparticles and nanomaterials that can properly relate to the physical scale of cell-cell and cell-niche interactions. This review summarizes the most relevant potential applications of nanoscale technologies to the field of stem cell therapy for the treatment of cardiovascular diseases.

Application of STEM/EELS to Plasmon-Related Effects in Optical Spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Camden, Jon

In this project we employed EELS/STEM to understand the near-field enhancements that drive current applications of plasmonic nanostructures. In particular, we explore the connection between optical and electron excitation of plasmon modes in metallic nanostructures: (1) Probing the structural parameters and dielectric properties of multimetallic nanoparticles; (2) Characterization of the near-electric-field enhancements obtained upon excitation of the localized surface plasmon resonance and understand the connection between electron- and photon-driven plasmons; (3) Understanding the behavior of molecules in plasmon-enhanced fields which is essential to emerging applications such as plasmon-assisted catalysis and solar energy harvesting.

Novel treatment strategies for feline chronic kidney disease: A critical look at the potential of mesenchymal stem cell therapy.

PubMed

Quimby, J M; Dow, S W

2015-06-01

Stem cell therapy is an innovative field of scientific investigation with tremendous potential for clinical application that holds promise for the treatment of a variety of diseases in veterinary medicine. Based on the known desirable properties of mesenchymal stem cells, the therapy has potential for treatment of both acute kidney injury and chronic kidney disease in cats. This review details terminology commonly used in this field of study, sources of mesenchymal stem cells and their proposed mechanism of action particularly as it relates to renal repair. Studies performed in rodent models of chronic kidney disease and feline clinical trial results are also summarized with the aim of providing an overview of the current status of this treatment modality and its potential for the future. Copyright © 2015 Elsevier Ltd. All rights reserved.

The self-renewal signaling pathways utilized by gastric cancer stem cells.

PubMed

Fu, Ying; Li, Hui; Hao, Xishan

2017-04-01

Gastric cancer is a leading cause of cancer-related mortality worldwide. Cancer stem cells are the source of tumor recurrence and metastasis. Self-renewal is a marker of cancer stem cells and also the basis of long-lasting survival and tumor progression. Although the mechanism of gastric cancer stem cell self-renewal is not clear, there are several signaling pathways and environmental factors known to be involved. This mini review describes recent developments in the self-renewal signaling pathway of gastric cancer stem cell research. Advancements made in this field of research will likely support the development of novel therapeutic strategies for gastric cancer.

Women's decision to major in STEM fields

NASA Astrophysics Data System (ADS)

Conklin, Stephanie

This paper explores the lived experiences of high school female students who choose to enter into STEM fields, and describes the influencing factors which steered these women towards majors in computer science, engineering and biology. Utilizing phenomenological methodology, this study seeks to understand the essence of women's decisions to enter into STEM fields and further describe how the decision-making process varies for women in high female enrollment fields, like biology, as compared with low enrollment fields like, computer science and engineering. Using Bloom's 3-Stage Theory, this study analyzes how relationships, experiences and barriers influenced women towards, and possibly away, from STEM fields. An analysis of women's experiences highlight that support of family, sustained experience in a STEM program during high school as well as the presence of an influential teacher were all salient factors in steering women towards STEM fields. Participants explained that influential teacher worked individually with them, modified and extended assignments and also steered participants towards coursework and experiences. This study also identifies factors, like guidance counselors as well as personal challenges, which inhibited participant's path to STEM fields. Further, through analyzing all six participants' experiences, it is clear that a linear model, like Bloom's 3-Stage Model, with limited ability to include potential barriers inhibited the ability to capture the essence of each participant's decision-making process. Therefore, a revised model with no linear progression which allows for emerging factors, like personal challenges, has been proposed; this model focuses on how interest in STEM fields begins to develop and is honed and then mastered. This study also sought to identify key differences in the paths of female students pursuing different majors. The findings of this study suggest that the path to computer science and engineering is limited. Computer science majors faced few, if any, challenges, hoped to use computers as a tool to innovate and also participated in the same computer science program. For female engineering students, the essence of their experience focused on interaction at a young age with an expert in an engineering-related field as well as a strong desire to help solve world problems using engineering. These participants were able to articulate clearly future careers. In contrast, biology majors, faced more challenges and were undecided about their future career goals. These results suggest that a longitudinal study focused on women pursuing engineering and computer science fields is warranted; this will hopefully allow these findings to be substantiated and also for refinement of the revised theoretical model.

Five Women in Science, Technology, Engineering, and Mathematics Majors: A Portraiture of Their Lived Experiences

ERIC Educational Resources Information Center

Torcivia, Patrice Prusko

2012-01-01

Numerous studies have addressed science, technology, engineering and mathematics (STEM) and their relation to education and gender ranging from elementary school pedagogy to career choices for traditional-aged college students. Little research has addressed nontraditional female students returning to the university to in the STEM fields. This…

The Shared Experiences: Facilitating Successful Transfer of Women and Underrepresented Minorities in STEM Fields

ERIC Educational Resources Information Center

Jackson, Dimitra Lynette; Starobin, Soko S.; Laanan, Frankie Santos

2013-01-01

This chapter addresses critical issues related to the transfer success of women and underrepresented minorities (URMs) in STEM disciplines and will highlight implications for fostering a successful transfer experience for these populations. For the purposes of this chapter, URMs is defined by the National Science Foundation to include African…

Examination of Factors Predicting Secondary Students' Interest in Tertiary STEM Education

ERIC Educational Resources Information Center

Chachashvili-Bolotin, Svetlana; Milner-Bolotin, Marina; Lissitsa, Sabina

2016-01-01

Based on the Social Cognitive Career Theory (SCCT), the study aims to investigate factors that predict students' interest in pursuing science, technology, engineering, and mathematics (STEM) fields in tertiary education both in general and in relation to their gender and socio-economic background. The results of the analysis of survey responses of…

Participation in Science and Technology: Young People's Achievement-Related Choices in Late-Modern Societies

ERIC Educational Resources Information Center

Boe, Maria Vetleseter; Henriksen, Ellen Karoline; Lyons, Terry; Schreiner, Camilla

2011-01-01

Young people's participation in science, technology, engineering and mathematics (STEM) is a matter of international concern. Studies and careers that require physical sciences and advanced mathematics are most affected by the problem and women in particular are under-represented in many STEM fields. This article views international research about…

Choosing a STEM Path: "Course-Sequencing in High School and Postsecondary Outcomes"

ERIC Educational Resources Information Center

Lee, Jonghwan; Judy, Justina

2011-01-01

The College Ambition Program (CAP) model was developed to support high schools in preparing their students to enter STEM fields. CAP includes four programmatic components: mentoring, course counseling and advising, college-related activities and workshops, and teacher professional development and instructional support. This study is part of a…

Breadth-Based Models of Women’s Underrepresentation in STEM Fields: An Integrative Commentary on Schmidt (2011) and Nye et al. (2012)

PubMed Central

Valla, Jeffrey M.; Ceci, Stephen J.

2014-01-01

Relative strength of math and verbal abilities and interests drive science, technology, engineering, and math (STEM) career choices more than absolute math ability alone. Having one dominant aptitude (e.g., for mathematics) increases the likelihood of a strong self-concept in that domain and decreases the likelihood of equivocation about career choices in comparison with individuals with equivalent mathematical aptitude who have comparable strength in non-math areas. Males are more likely than females to have an asymmetrical cognitive profile of higher aptitude in math relative to verbal domains. Together, these two points suggest that the academic and career pursuits of high math ability males may be attributable to their narrower options among STEM fields, whereas females’ more symmetrical cognitive profile means their math and verbal interests compete in the formation of their ability self-concept and, hence, in their broader career choices. Such equivocation about STEM careers is in fact already evident in girls with high math aptitude as early as junior high school. Thus, we argue that asymmetry in interests and aptitudes is an underappreciated factor in sex differences in career choice. To the extent this is true, focusing on strengthening young women’s STEM-related abilities and ability self-concepts to increase female STEM representation may be an unproductive approach; to increase representation, it may be more effective to focus on harvesting the potential of those girls and women whose breadth of interest and high ability spans social/verbal and spatial/numerical domains. The use of interventions that play to this greater breadth by socially contextualizing STEM is one potential solution. PMID:25076979

Gender and grade level differences in interest, perceived personal capacity, and involvement in technology and engineering-related activities

NASA Astrophysics Data System (ADS)

Weber, Katherine

Society has become increasingly technological, demanding that all citizens have a level of technological literacy. In order for this to occur, both males and females must participate in technology-related activities to achieve an adequate level of technological literacy. Despite individual and organizational efforts, females continue to be underrepresented in STEM-related occupations. This is especially true in many engineering-related fields. Jolly, Campbell and Perlman (2004) devised the Engagement, Capacity, and Continuity (ECC) Trilogy. With each factor of the trilogy in place, Jolly et al. found that female representation increased in STEM. The purpose of this study was to identify whether Jolly, Campbell, and Perlman's (2004) Engagement, Capacity, and Continuity Trilogy could be utilized by teachers in technology and engineering program settings to examine their students' interest (engagement), perceived personal capacity (capacity), as well as participation in technology and engineering-related activities (continuity). This descriptive study surveyed 556 female and male middle school and high school students enrolled in Technology and Engineering classes. The results of this study revealed that when students indicated a high interest and a high perceived personal capacity, and when they participated in technology and engineering-related activities, they also indicated an interest in pursuing a career in engineering. The results also revealed that the male students continued to be encouraged by technology and engineering teachers, parents, and counselors to pursue a career in engineering more than female students. This startling finding should draw some concern; both males and females should be equally encouraged to consider engineering as a career. Technology and engineering teachers should implement activities that appeal to both males and females. Parents should encourage their daughters to participate in informal learning opportunities to nurture their daughters' interest in STEM-related areas. Counselors should gain an awareness of the scope and diversity of different engineering fields so they can advise both male and female students to consider careers in engineering. In order for the United States to be competitive and innovative at the global level, female representation and contributions in STEM fields must increase. Key Words: GENDER, ENGAGEMENT, INTEREST, PERCEIVED PERSONAL CAPACITY, TECHNOLOGY AND ENGINEERING ACTIVITIES, WISCONSIN, STEM, AFTERSCHOOL ACTIVITIES.

Ethnic variation in gender-STEM stereotypes and STEM participation: an intersectional approach.

PubMed

O'Brien, Laurie T; Blodorn, Alison; Adams, Glenn; Garcia, Donna M; Hammer, Elliott

2015-04-01

Stereotypes associating men and masculine traits with science, technology, engineering, and mathematics (STEM) fields are ubiquitous, but the relative strength of these stereotypes varies considerably across cultures. The present research applies an intersectional approach to understanding ethnic variation in gender-STEM stereotypes and STEM participation within an American university context. African American college women participated in STEM majors at higher rates than European American college women (Study 1, Study 2, and Study 4). Furthermore, African American women had weaker implicit gender-STEM stereotypes than European American women (Studies 2-4), and ethnic differences in implicit gender-STEM stereotypes partially mediated ethnic differences in STEM participation (Study 2 and Study 4). Although African American men had weaker implicit gender-STEM stereotypes than European American men (Study 4), ethnic differences between men in STEM participation were generally small (Study 1) or nonsignificant (Study 4). We discuss the implications of an intersectional approach for understanding the relationship between gender and STEM participation. (c) 2015 APA, all rights reserved).

Amnion-derived stem cells: in quest of clinical applications

PubMed Central

2011-01-01

In the promising field of regenerative medicine, human perinatal stem cells are of great interest as potential stem cells with clinical applications. Perinatal stem cells could be isolated from normally discarded human placentae, which are an ideal cell source in terms of availability, the fewer number of ethical concerns, less DNA damage, and so on. Numerous studies have demonstrated that some of the placenta-derived cells possess stem cell characteristics like pluripotent differentiation ability, particularly in amniotic epithelial (AE) cells. Term human amniotic epithelium contains a relatively large number of stem cell marker-positive cells as an adult stem cell source. In this review, we introduce a model theory of why so many AE cells possess stem cell characteristics. We also describe previous work concerning the therapeutic applications and discuss the pluripotency of the AE cells and potential pitfalls for amnion-derived stem cell research. PMID:21596003

STEM Education.

PubMed

Xie, Yu; Fang, Michael; Shauman, Kimberlee

2015-08-01

Improving science, technology, engineering, and mathematics (STEM) education, especially for traditionally disadvantaged groups, is widely recognized as pivotal to the U.S.'s long-term economic growth and security. In this article, we review and discuss current research on STEM education in the U.S., drawing on recent research in sociology and related fields. The reviewed literature shows that different social factors affect the two major components of STEM education attainment: (1) attainment of education in general, and (2) attainment of STEM education relative to non-STEM education conditional on educational attainment. Cognitive and social psychological characteristics matter for both major components, as do structural influences at the neighborhood, school, and broader cultural levels. However, while commonly used measures of socioeconomic status (SES) predict the attainment of general education, social psychological factors are more important influences on participation and achievement in STEM versus non-STEM education. Domestically, disparities by family SES, race, and gender persist in STEM education. Internationally, American students lag behind those in some countries with less economic resources. Explanations for group disparities within the U.S. and the mediocre international ranking of US student performance require more research, a task that is best accomplished through interdisciplinary approaches.

STEM Education

PubMed Central

Xie, Yu; Fang, Michael; Shauman, Kimberlee

2015-01-01

Improving science, technology, engineering, and mathematics (STEM) education, especially for traditionally disadvantaged groups, is widely recognized as pivotal to the U.S.’s long-term economic growth and security. In this article, we review and discuss current research on STEM education in the U.S., drawing on recent research in sociology and related fields. The reviewed literature shows that different social factors affect the two major components of STEM education attainment: (1) attainment of education in general, and (2) attainment of STEM education relative to non-STEM education conditional on educational attainment. Cognitive and social psychological characteristics matter for both major components, as do structural influences at the neighborhood, school, and broader cultural levels. However, while commonly used measures of socioeconomic status (SES) predict the attainment of general education, social psychological factors are more important influences on participation and achievement in STEM versus non-STEM education. Domestically, disparities by family SES, race, and gender persist in STEM education. Internationally, American students lag behind those in some countries with less economic resources. Explanations for group disparities within the U.S. and the mediocre international ranking of US student performance require more research, a task that is best accomplished through interdisciplinary approaches. PMID:26778893

The Gender-Equality Paradox in Science, Technology, Engineering, and Mathematics Education.

PubMed

Stoet, Gijsbert; Geary, David C

2018-04-01

The underrepresentation of girls and women in science, technology, engineering, and mathematics (STEM) fields is a continual concern for social scientists and policymakers. Using an international database on adolescent achievement in science, mathematics, and reading ( N = 472,242), we showed that girls performed similarly to or better than boys in science in two of every three countries, and in nearly all countries, more girls appeared capable of college-level STEM study than had enrolled. Paradoxically, the sex differences in the magnitude of relative academic strengths and pursuit of STEM degrees rose with increases in national gender equality. The gap between boys' science achievement and girls' reading achievement relative to their mean academic performance was near universal. These sex differences in academic strengths and attitudes toward science correlated with the STEM graduation gap. A mediation analysis suggested that life-quality pressures in less gender-equal countries promote girls' and women's engagement with STEM subjects.

The Impact of Length of Engagement in After-School STEM Programs on Middle School Girls

NASA Astrophysics Data System (ADS)

Cupp, Garth Meichel

An underrepresentation of females exists in the STEM fields. In order to tackle this issue, work begins early in the education of young women to ensure they are interested and have the confidence to gain a career in the STEM fields. It is important to engage girls in STEM opportunities in and out of school to ignite their interest and build their confidence. Brigid Barron's learning ecology perspective shows that girls pursuing STEM outside of the classroom is critical to their achievement in the STEM pipeline. This study investigated the impact after-school STEM learning opportunities have on middle school girls by investigating (a) how the length of engagement in after-school programs can affect the confidence of female students in their science and math abilities; (b) how length of engagement in after-school programs can affect the interest of female students in attaining a career in STEM; (c) how length of engagement in after-school programs can affect interest in science and math classes; and (d) how length of engagement can affect how female students' view gender parity in the STEM workforce. The major findings revealed no statistical significance when comparing confidence in math or science abilities or the perception that gender plays a role in attaining a career in STEM. The findings revealed statistical significance in the areas when comparing length of engagement in the girls' interest in their math class and attaining a career in three of the four STEM fields: science, technology, and engineering. The findings showed that multiple terms of engagement in the after-school STEM programs appear to be an effective catalyst to maintain the interest of girls pursuing STEM-related careers, in addition to allowing their interest in a topic to provide a new lens for the way they see their math work during the school day. The implications of this study show that schools must engage middle school girls who are interested in STEM in a multitude of settings, including outside of the classroom in order to maintain engagement in the STEM pipeline.

(Un)Hidden Figures: A Synthesis of Research Examining the Intersectional Experiences of Black Women and Girls in STEM Education

ERIC Educational Resources Information Center

Ireland, Danyelle T.; Freeman, Kimberley Edelin; Winston-Proctor, Cynthia E.; DeLaine, Kendra D.; McDonald Lowe, Stacey; Woodson, Kamilah M.

2018-01-01

In this chapter, we argue that intersectionality is a theoretical and methodological framework by which education researchers can critically examine why and how students in STEM fields who are members of intersecting marginalized groups have distinctive experiences related to their social identities, other psychological processes, and educational…

A Mixed Methods Study on Evaluations of Virginia's STEM-Focused Governor's Schools

ERIC Educational Resources Information Center

Stith, Krista M.

2017-01-01

Significant emphasis is currently placed on STEM education as a vehicle to encourage American youth to enter science, technology, engineering, and math-related professions. Gifted students are a natural resource of future innovators for these fields; however gifted programs are largely overlooked for program support. Since 1973, the Virginia…

Inspiring Careers in STEM and Healthcare Fields through Medical Simulation Embedded in High School Science Education

ERIC Educational Resources Information Center

Berk, Louis J.; Muret-Wagstaff, Sharon L.; Goyal, Riya; Joyal, Julie A.; Gordon, James A.; Faux, Russell; Oriol, Nancy E.

2014-01-01

The most effective ways to promote learning and inspire careers related to science, technology, engineering, and mathematics (STEM) remain elusive. To address this gap, we reviewed the literature and designed and implemented a high-fidelity, medical simulation-based Harvard Medical School MEDscience course, which was integrated into high school…

Career Outcomes of STEM and Non-STEM College Graduates: Persistence in Majored-Field and Influential Factors in Career Choices

ERIC Educational Resources Information Center

Xu, Yonghong Jade

2013-01-01

Using data from a nationally representative, longitudinal survey of college graduates, this study examines student transition from college to their chosen career paths and identifies factors influencing college graduates' choosing an occupation related to ones' undergraduate major. Within the context of expanded econometric framework a wide range…

Push and Pull: The Influence of Race/Ethnicity on Agency in Doctoral Student Career Advancement

ERIC Educational Resources Information Center

Jaeger, Audrey J.; Mitchall, Allison; O'Meara, KerryAnn; Grantham, Ashley; Zhang, Jingjing; Eliason, Jennifer; Cowdery, Kelly

2017-01-01

This study examined and enriched our understanding of the career choice process for doctoral students of color in science, technology, engineering, and math (STEM) fields. In addition, it explored the challenges facing all doctoral students in STEM in understanding and making meaning of diversity as it relates to individual perspectives and…

International Baccalaureate Diploma Programs (IBDP) in Oklahoma: A Mixed Methods Study

ERIC Educational Resources Information Center

Hood, Susan J.

2012-01-01

Scope and Method of Study: As Oklahoma grows in technical markets, the need for science, technology, engineering, and mathematics (STEM) educated individuals will continue to increase. Our focus in aviation should be at focusing to attract students into STEM related fields, however, a stronger focus needs to be in retaining the top tier category…

Trends in Gender Segregation in the Choice of Science and Engineering Majors*

PubMed Central

Mann, Allison; DiPrete, Thomas A.

2013-01-01

Numerous theories have been put forward for the high and continuing levels of gender segregation in science, technology, engineering, and mathematics (STEM) fields, but research has not systematically examined the extent to which these theories for the gender gap are consistent with actual trends. Using both administrative data and four separate longitudinal studies sponsored by the U.S. Department of Education’s National Center for Education Statistics (NCES), we evaluate several prominent explanations for the persisting gender gap in STEM fields related to mathematics performance and background and general life goals, and find that none of them are empirically satisfactory. Instead, we suggest that the structure of majors and their linkages to professional training and careers may combine with gender differences in educational goals to influence the persisting gender gap in STEM fields. An analysis of gendered career aspirations, course-taking patterns, and pathways to medical and law school supports this explanation. PMID:24090849

Financial Barriers to STEM Study in College: Causal Effect Estimates of Need-Based Grants on the Pursuit and Completion of Courses and Degrees in STEM Fields

ERIC Educational Resources Information Center

Castleman, Benjamin L.; Long, Bridget Terry; Mabel, Zachary A.

2014-01-01

The fastest growing supply of jobs in the United States today is in Science, Technology, Engineering, and Mathematics (STEM) fields. Yet despite the availability of work in STEM, there is not a sufficient supply of workers to fill open positions. Amidst the growing demand for STEM workers, educational achievement and attainment in STEM fields in…

Comparison of Stem Map Developed from Crown Geometry Allometry Linked Census Data to Airborne and Terrestrial Lidar at Harvard Forest, MA

NASA Astrophysics Data System (ADS)

Sullivan, F.; Palace, M. W.; Ducey, M. J.; David, O.; Cook, B. D.; Lepine, L. C.

2014-12-01

Harvard Forest in Petersham, MA, USA is the location of one of the temperate forest plots established by the Center for Tropical Forest Science (CTFS) as a joint effort with Harvard Forest and the Smithsonian Institute's Forest Global Earth Observatory (ForestGEO) to characterize ecosystem processes and forest dynamics. Census of a 35 ha plot on Prospect Hill was completed during the winter of 2014 by researchers at Harvard Forest. Census data were collected according to CTFS protocol; measured variables included species, stem diameter, and relative X-Y locations. Airborne lidar data were collected over the censused plot using the high spatial resolution Goddard LiDAR, Hyperspectral, and Thermal sensor package (G-LiHT) during June 2012. As part of a separate study, 39 variable radius plots (VRPs) were randomly located and sampled within and throughout the Prospect Hill CTFS/ForestGEO plot during September and October 2013. On VRPs, biometric properties of trees were sampled, including species, stem diameter, total height, crown base height, crown radii, and relative location to plot centers using a 20 Basal Area Factor prism. In addition, a terrestrial-based lidar scanner was used to collect one lidar scan at plot center for 38 of the 39 VRPs. Leveraging allometric equations of crown geometry and tree height developed from 374 trees and 16 different species sampled on 39 VRPs, a 3-dimensional stem map will be created using the Harvard Forest ForestGEO Prospect Hill census. Vertical and horizontal structure of 3d field-based stem maps will be compared to terrestrial and airborne lidar scan data. Furthermore, to assess the quality of allometric equations, a 2d canopy height raster of the field-based stem map will be compared to a G-LiHT derived canopy height model for the 35 ha census plot. Our automated crown delineation methods will be applied to the 2d representation of the census stem map and the G-LiHT canopy height model. For future work related to this study, high quality field-based stem maps with species and crown geometry information will allow for better comparisons and interpretations of individual tree spectra from the G-LiHT hyperspectral sensor as estimated by automated crown delineation of the G-LiHT lidar canopy height model.

Ocular stem cells: a status update!

PubMed Central

2014-01-01

Stem cells are unspecialized cells that have been a major focus of the field of regenerative medicine, opening new frontiers and regarded as the future of medicine. The ophthalmology branch of the medical sciences was the first to directly benefit from stem cells for regenerative treatment. The success stories of regenerative medicine in ophthalmology can be attributed to its accessibility, ease of follow-up and the eye being an immune-privileged organ. Cell-based therapies using stem cells from the ciliary body, iris and sclera are still in animal experimental stages but show potential for replacing degenerated photoreceptors. Limbal, corneal and conjunctival stem cells are still limited for use only for surface reconstruction, although they might have potential beyond this. Iris pigment epithelial, ciliary body epithelial and choroidal epithelial stem cells in laboratory studies have shown some promise for retinal or neural tissue replacement. Trabecular meshwork, orbital and sclera stem cells have properties identical to cells of mesenchymal origin but their potential has yet to be experimentally determined and validated. Retinal and retinal pigment epithelium stem cells remain the most sought out stem cells for curing retinal degenerative disorders, although treatments using them have resulted in variable outcomes. The functional aspects of the therapeutic application of lenticular stem cells are not known and need further attention. Recently, embryonic stem cell-derived retinal pigment epithelium has been used for treating patients with Stargardts disease and age-related macular degeneration. Overall, the different stem cells residing in different components of the eye have shown some success in clinical and animal studies in the field of regenerative medicine. PMID:25158127

Development of a Short-Form Measure of Science and Technology Self-efficacy Using Rasch Analysis

NASA Astrophysics Data System (ADS)

Lamb, Richard L.; Vallett, David; Annetta, Leonard

2014-10-01

Despite an increased focus on science, technology, engineering, and mathematics (STEM) in U.S. schools, today's students often struggle to maintain adequate performance in these fields compared with students in other countries (Cheek in Thinking constructively about science, technology, and society education. State University of New York, Albany, 1992; Enyedy and Goldberg 2004; Mandinach and Lewis 2006). In addition, despite considerable pressure to promote the placement of students into STEM career fields, U.S. placement is relatively low (Sadler et al. in Sci Educ 96(3):411-427, 2012; Subotnik et al. in Identifying and developing talent in science, technology, engineering, and mathematics (STEM): an agenda for research, policy and practice. International handbook, part XII, pp 1313-1326, 2009). One explanation for the decline of STEM career placement in the U.S. rests with low student affect concerning STEM concepts and related content, especially in terms of self-efficacy. Researchers define self-efficacy as the internal belief that a student can succeed in learning, and that understanding student success lies in students' externalized actions or behaviors (Bandura in Psychol Rev 84(2):191-215, 1977). Evidence suggests that high self-efficacy in STEM can result in student selection of STEM in later educational endeavors, culminating in STEM career selection (Zeldin et al. in J Res Sci Teach 45(9):1036-1058, 2007). However, other factors such as proficiency play a role as well. The lack of appropriate measures of self-efficacy can greatly affect STEM career selection due to inadequate targeting of this affective trait and loss of opportunity for early intervention by educators. Lack of early intervention decreases selection of STEM courses and careers (Valla and Williams in J Women Minor Sci Eng 18(1), 2012; Lent et al. in J Couns Psychol 38(4), 1991). Therefore, this study developed a short-form measure of self-efficacy to help identify students in need of intervention.

A Study of the Experience of Female African-American Seventh Graders in a Science, Technology, Engineering, and Math (STEM) Afterschool Program

NASA Astrophysics Data System (ADS)

Hinds, Beverley Fiona

The purpose of this qualitative study was to determine what inspires or leads seventh-grade African-American girls toward an interest in STEM, to characterize and describe the context of an out-of-school STEM learning environment, explore the impact on the seventh- grade African-American girls who participated in the program as it relates to individual STEM identity, and identify personal and academic experiences of seventh-grade African- American girls that contribute to the discouragement or pursuit of science and math-related academic pathways and careers. Notable findings in this study included the following: 1. Participants were interested in STEM and able to identify both external and internal influences that supported their involvement and interest in STEM activities. External influences expanded and elevated exposure to STEM experiences. 2. The MJS program provided an opportunity for participants to overcome challenges related to science and math knowledge and skills in school. 3. The MJS program increased levels of interest in STEM for the participants. 4. All participants increased their capacity to demonstrate increased knowledge in STEM content as a result of the learning experiences within the MJS program, and participants transferred this knowledge to experiences outside of the program including school. 5. The STEM learning environment provided multiple opportunities for participants to meet high expectation and access to engaging activities within a supportive, well-managed setting. 6. The MJS program participants demonstrated behaviors related to building a STEM identity through the components described by Carlone and Johnson (2007), including recognition-internal and external acknowledgement of being a STEM person; competence-demonstrating an understanding of STEM content; and performance-publically exhibiting STEM knowledge and skills. The findings in this study suggested that African-American seventh-grade girls interested in STEM are inspired and encouraged to participate in STEM by both internal and external factors. Highly effective afterschool STEM programs increase interest, knowledge and skills in STEM. The capacity for building a STEM identity was expanded as explored/measured by the components of recognition, competence, and performance (Carlone & Johnson, 2007). The learning environment conditions and support for building a STEM identity enhance the pursuit of STEM-related fields for African-American middle school girls. Application of these factors add to the potential for a decrease in the gap of representation of African-American women engaged in STEM. Future studies may explore how African-American middle schools girls interested in STEM construct identity as it relates to STEM, racial, and gender identity development and how the mentoring experience in afterschool STEM programs impacts the career choices of pre-teaching students.

Staying in STEM or changing course: Do natives and immigrants pursue the path of least resistance?

PubMed

Han, Siqi

2016-07-01

This paper examines why Science, Technology, Engineering and Math (STEM) fields are becoming "immigrant" fields of study as native students shift from STEM fields to law, medicine and business. Using data from the 2010 National Survey of College Graduates, the analyses find that foreign college-educated immigrants with STEM degrees tend to remain in STEM fields, while natives are more likely to shift from STEM fields to law, medicine and business in graduate school. Among those who moved into law, medicine and business, the gains in earnings are larger for natives than for foreign educated immigrants. These results have important implications for the social mobility of highly educated natives and immigrants. Copyright © 2016 Elsevier Inc. All rights reserved.

The Underrepresentation of Women in the Engineering Element of STEM Occupations and Influencers Contributing to the Persistent Gap

ERIC Educational Resources Information Center

Holl, David

2017-01-01

Within Science, Technology, Engineering, and Mathematics (STEM) careers fields, the representation of women remains at an inequitable level when compared to men and to women's representation in other professions. Given the current state of women representing 52% of the professional and management-related workforce (U.S. Bureau of Labor and…

"Failure Is a Major Component of Learning Anything": The Role of Failure in the Development of STEM Professionals

NASA Astrophysics Data System (ADS)

Simpson, Amber; Maltese, Adam

2017-04-01

The term failure typically evokes negative connotations in educational settings and is likely to be accompanied by negative emotional states, low sense of confidence, and lack of persistence. These negative emotional and behavioral states may factor into an individual not pursuing a degree or career in science, technology, engineering, or mathematics (STEM). This is of particular concern considering the low number of women and underrepresented minorities pursing and working in a STEM field. Utilizing interview data with professionals across STEM, we sought to understand the role failure played in the persistence of individuals who enter and pursue paths toward STEM-related careers. Findings highlighted how participants' experiences with failure (1) shaped their outlooks or views of failure, (2) shaped their trajectories within STEM, and (3) provided them with additional skills or qualities. A few differences based on participants' sex, field, and highest degree also manifested in our analysis. We expect the results from this study to add research-based results to the current conversation around whether experiences with failure should be part of formal and informal educational settings and standards-based practices.

Stem cell senescence. Effects of REAC technology on telomerase-independent and telomerase-dependent pathways.

PubMed

Rinaldi, S; Maioli, M; Pigliaru, G; Castagna, A; Santaniello, S; Basoli, V; Fontani, V; Ventura, C

2014-09-16

Decline in the gene expression of senescence repressor Bmi1, and telomerase, together with telomere shortening, underlay senescence of stem cells cultured for multiple passages. Here, we investigated whether the impairment of senescence preventing mechanisms can be efficiently counteracted by exposure of human adipose-derived stem cells to radio electric asymmetrically conveyed fields by an innovative technology, named Radio Electric Asymmetric Conveyer (REAC). Due to REAC exposure, the number of stem cells positively stained for senescence associated β-galactosidase was significantly reduced along multiple culturing passages. After a 90-day culture, REAC-treated cells exhibited significantly higher transcription of Bmi1 and enhanced expression of other stem cell pluripotency genes and related proteins, compared to unexposed cells. Transcription of the catalytic telomerase subunit (TERT) was also increased in REAC-treated cells at all passages. Moreover, while telomere shortening occurred at early passages in both REAC-treated and untreated cells, a significant rescue of telomere length could be observed at late passages only in REAC-exposed cells. Thus, REAC-asymmetrically conveyed radio electric fields acted on a gene and protein expression program of both telomerase-independent and telomerase-dependent patterning to optimize stem cell ability to cope with senescence progression.

Key Action Items for the Stem Cell Field: Looking Ahead to 2014

PubMed Central

Knoepfler, Paul S.

2013-01-01

Abstract The stem cell field is at a critical juncture in late 2013. We find ourselves buoyed by building momentum for both transformative basic science discoveries and clinical translation of stem cells. Cellular reprogramming has given the field exciting new avenues as well. The overall prospect of novel stem cell–based therapies becoming a reality for patients in the coming years has never seemed higher. At the same time, we face serious challenges. Some of these challenges, such as stem cell tourism, are familiar to us, although even those are evolving in ways that require adaptability and action by the stem cell field. Other new challenges are also emerging, including an urgent need for formal physician training in stem cells, regulatory compliance balanced with innovation and U.S. Food and Drug Administration reform, and savvy educational outreach. Looking ahead to 2014, both the challenges and opportunities for the stem cell field require a proactive, thoughtful approach to maximize the potential for a positive impact from stem cell advances. In this study, I discuss the key action items for the field as we look ahead to the coming year and beyond. PMID:24147571

Key action items for the stem cell field: looking ahead to 2014.

PubMed

Knoepfler, Paul S

2013-12-01

The stem cell field is at a critical juncture in late 2013. We find ourselves buoyed by building momentum for both transformative basic science discoveries and clinical translation of stem cells. Cellular reprogramming has given the field exciting new avenues as well. The overall prospect of novel stem cell-based therapies becoming a reality for patients in the coming years has never seemed higher. At the same time, we face serious challenges. Some of these challenges, such as stem cell tourism, are familiar to us, although even those are evolving in ways that require adaptability and action by the stem cell field. Other new challenges are also emerging, including an urgent need for formal physician training in stem cells, regulatory compliance balanced with innovation and U.S. Food and Drug Administration reform, and savvy educational outreach. Looking ahead to 2014, both the challenges and opportunities for the stem cell field require a proactive, thoughtful approach to maximize the potential for a positive impact from stem cell advances. In this study, I discuss the key action items for the field as we look ahead to the coming year and beyond.

Redefining Science, Technology, Engineering, and Mathematics (STEM) Educational Opportunities for Underserved and Underrepresented Students at NASA

ERIC Educational Resources Information Center

Hackler, Amanda Smith

2011-01-01

Underserved and underrepresented students consistently leave science, technology, engineering, and mathematics (STEM) degree fields to pursue less demanding majors. This perpetual problem slowed the growth in STEM degree fields (United States Department of Labor, 2007). Declining enrollment in STEM degree fields among underserved and…

Do STEM fields need a makeover?: The effect of role model femininity on men and women's interest in STEM

NASA Astrophysics Data System (ADS)

Howard, Carolynn

Women continue to be underrepresented in science, technology, engineering, and mathematics (STEM) fields. This lack of women is problematic because it diminishes perspective, input, and expertise that women could provide. Consequently, this thesis examined the benefits of exposure to peer role models for increasing women's interest in STEM, which may ultimately lead more women to enter STEM fields. The role model research to date has amassed considerable evidence showing that role model exposure is beneficial; yet, questions still remain about what makes these role models effective. Accordingly, this thesis investigated whether feminine female role models increase women's interest in STEM and improve their perceptions of female STEM role models relative to "neutral" female role models. Across three experiments men and women were exposed to role models and their interest in STEM was measured. All experiments exposed participants to one of three articles about a peer role model (a female role model who embodies femininity (e.g. wears makeup), a female role model who has gender neutral qualities/behaviors [e.g., works hard], or a male role model who embodies neutral traits) and Experiments 2 and 3 had a fourth control condition in which participants read about the history of SDSU (a control condition). In the first two experiments interest in physics was measured using an adapted version of the STEM Career Interest Survey (CIS). Experiment 3 used an adapted version of the STEM CIS scale, but measured overall interest in STEM by including subscales for each of the four STEM areas with a composite score serving as the primary dependent variable. Experiments 1 and 2 demonstrated that women's interest in physics was no different than men's after exposure to a feminine female role model compared to a neutral female and neutral male role model. Furthermore, women's interest in physics was greater in the feminine condition compared to all other conditions for the first two experiments, thus demonstrating that a competent feminine role model may be useful in piquing women's interest in physics. Experiment 3, however, did not display this pattern for women's interest in STEM overall.

[The fundamental mechanisms of metastatic spread and chemotherapy resistance in lung cancer].

PubMed

Tomuleasa, Ciprian; Kacso, Gabriel; Soritau, Olga; Susman, Sergiu; Petrushev, Bobe; Aldea, Mihaela; Buiga, Rareş; Irimie, Alexandru

2011-01-01

Lung cancer is the leading cause of cancer-related death in the European Union and the United States, accounting for about one third of all cancer deaths. Primary lung cancer may arise from the central (bronchial) or peripheral (bronchiolo-alveolar) compartment of the lung, but the origins of the different histological types of primary lung tumours are not well understood and described in medical literature. Current investigation in the field of cancer research have focused on the "cancer stem cell" hypothesis as stem cells are belived to be crucial players in the homeostasis of all adult tissues. Even if the role of stem cells in lung carcinogenesis is not clear yet, numerous studies indicate that lung cancer is not the result of a sudden transforming event, but of a multistep process of molecular changes of the primordial stem cell niche, leading to the development of noeplasia. In the current review, we present state-of-the-art research in the field of lung stem cell biology, with a special emphasis on lung cancer emergence, development, metastasis and multidrug resistance.

Allogeneic Stem Cell Transplantation: A Historical and Scientific Overview.

PubMed

Singh, Anurag K; McGuirk, Joseph P

2016-11-15

The field of hematopoietic stem cell transplant (HSCT) has made ground-breaking progress in the treatment of many malignant and nonmalignant conditions. It has also pioneered the concepts of stem cell therapy and immunotherapy as a tool against cancer. The success of transplant for hematologic malignancies derives both from the ability to treat patients with intensive chemoradiotherapy and from potent graft-versus-leukemia (GVL) effects mediated by donor immunity. Additionally, HSCT has been a curative therapy for several nonmalignant hematologic disorders through the provision of donor-derived hematopoiesis and immunity. Preclinical and clinical research in the field has contributed to an advanced understanding of histocompatibility, graft-versus-host disease (GVHD), GVL effect, and immune reconstitution after transplant. Improved donor selection, tailored conditioning regimens, and better supportive care have helped reduce transplant-related morbidity and mortality and expanded access. The development of unrelated donor registries and increased utilization of cord blood and partially matched related donor transplants have ensured a donor for essentially everyone who needs a transplant. However, significant barriers still remain in the form of disease relapse, GVHD infectious complications, and regimen-related toxicities. Recent developments in the field of cellular therapy are expected to further improve the efficacy of transplant. In this review, we discuss the current science of HSCT from a historical perspective, highlighting major discoveries. We also speculate on future directions in this field. Cancer Res; 76(22); 6445-51. ©2016 AACR. ©2016 American Association for Cancer Research.

The Application of Nanomaterials in Stem Cell Therapy for Some Neurological Diseases.

PubMed

Zhang, Guilong; Khan, Ahsan Ali; Wu, Hao; Chen, Lukui; Gu, Yuchun; Gu, Ning

2018-02-08

Stem cell therapy provides great promising therapeutic benefits for various neurological disorders. Cell transplantation has emerged as cell replacement application for nerve damage. Recently, nanomaterials obtain wide development in various industrial and medical fields, and nanoparticles have been applied in the neurological field for tracking and treating nervous system diseases. Combining stem cells with nanotechnology has raised more and more attentions; and it has demonstrated that the combination has huge effects on clinical diagnosis and therapeutics in multiple central nervous system diseases, meanwhile, improves prognosis. The aim of this review was to give a brief overview of the application of nanomaterials in stem cell therapy for neurological diseases. Nanoparticles not only promote stem cell proliferation and differentiation in vitro or in vivo, but also play dominant roles on stem cell imaging and tracking. Furthermore, via delivering genes or drugs, nanoparticles can participate in stem cell therapeutic applications for various neurological diseases, such as ischemic stroke, spinal cord injury (SCI), multiple sclerosis (MS), Parkinson's disease (PD), Alzheimer's disease (AD) and gliomas. However, nanoparticles have potential cytotoxic effects on nerve cells, which are related to their physicochemical properties. Nano-stem cell-based therapy as a promising strategy has the ability to affect neuronal repair and regeneration in the central nervous system. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Integrated STEM in secondary education: A case study

NASA Astrophysics Data System (ADS)

De Meester, Jolien; Knipprath, Heidi; Thielemans, Jan; De Cock, Mieke; Langie, Greet; Dehaene, Wim

2016-05-01

Despite many opportunities to study STEM (Science, Technology, Engineering & Mathematics) in Flemish secondary education, only a minority of pupils are actually pursuing STEM fields in higher education and jobs. One reason could be that they do not see the relevance of science and mathematics. In order to draw their pupils' interest in STEM, a Belgian school started a brand new initiative: the school set up and implemented a first year course that integrates various STEM disciplines, hoping to provide an answer to the question pupils often ask themselves about the need to study math and science. The integrated curriculum was developed by the school's teachers and a STEM education research group of the University of Leuven. To examine the pupils' attitude towards STEM and STEM professions and their notion of relevance of STEM at the end of this one-year course, a post-test was administered to the group of pupils who attended the integrated STEM course (the experimental group) and to a group of pupils that took traditional, non-integrated STEM courses (the control group). The results reveal that attending the integrated STEM course is significantly related to pupils' interest in STEM and notion of relevance of STEM. Another post-test was administered only to the experimental group to investigate pupils' understanding of math and physics concepts and their relation when taught in an integrated way. The results reveal that the pupils have some conceptual understanding and can, to a certain extent, make a transfer of concepts across different STEM disciplines. However, the test results did point out that some additional introductory training in pure math context is needed.

All STEM fields are not created equal: People and things interests explain gender disparities across STEM fields

PubMed Central

Su, Rong; Rounds, James

2015-01-01

The degree of women's underrepresentation varies by STEM fields. Women are now overrepresented in social sciences, yet only constitute a fraction of the engineering workforce. In the current study, we investigated the gender differences in interests as an explanation for the differential distribution of women across sub-disciplines of STEM as well as the overall underrepresentation of women in STEM fields. Specifically, we meta-analytically reviewed norm data on basic interests from 52 samples in 33 interest inventories published between 1964 and 2007, with a total of 209,810 male and 223,268 female respondents. We found gender differences in interests to vary largely by STEM field, with the largest gender differences in interests favoring men observed in engineering disciplines (d = 0.83–1.21), and in contrast, gender differences in interests favoring women in social sciences and medical services (d = −0.33 and −0.40, respectively). Importantly, the gender composition (percentages of women) in STEM fields reflects these gender differences in interests. The patterns of gender differences in interests and the actual gender composition in STEM fields were explained by the people-orientation and things-orientation of work environments, and were not associated with the level of quantitative ability required. These findings suggest potential interventions targeting interests in STEM education to facilitate individuals' ability and career development and strategies to reform work environments to better attract and retain women in STEM occupations. PMID:25762964

All STEM fields are not created equal: People and things interests explain gender disparities across STEM fields.

PubMed

Su, Rong; Rounds, James

2015-01-01

The degree of women's underrepresentation varies by STEM fields. Women are now overrepresented in social sciences, yet only constitute a fraction of the engineering workforce. In the current study, we investigated the gender differences in interests as an explanation for the differential distribution of women across sub-disciplines of STEM as well as the overall underrepresentation of women in STEM fields. Specifically, we meta-analytically reviewed norm data on basic interests from 52 samples in 33 interest inventories published between 1964 and 2007, with a total of 209,810 male and 223,268 female respondents. We found gender differences in interests to vary largely by STEM field, with the largest gender differences in interests favoring men observed in engineering disciplines (d = 0.83-1.21), and in contrast, gender differences in interests favoring women in social sciences and medical services (d = -0.33 and -0.40, respectively). Importantly, the gender composition (percentages of women) in STEM fields reflects these gender differences in interests. The patterns of gender differences in interests and the actual gender composition in STEM fields were explained by the people-orientation and things-orientation of work environments, and were not associated with the level of quantitative ability required. These findings suggest potential interventions targeting interests in STEM education to facilitate individuals' ability and career development and strategies to reform work environments to better attract and retain women in STEM occupations.

Therapeutic potential of dental stem cells

PubMed Central

Chalisserry, Elna Paul; Nam, Seung Yun; Park, Sang Hyug; Anil, Sukumaran

2017-01-01

Stem cell biology has become an important field in regenerative medicine and tissue engineering therapy since the discovery and characterization of mesenchymal stem cells. Stem cell populations have also been isolated from human dental tissues, including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, dental follicle progenitor cells, and periodontal ligament stem cells. Dental stem cells are relatively easily obtainable and exhibit high plasticity and multipotential capabilities. The dental stem cells represent a gold standard for neural-crest-derived bone reconstruction in humans and can be used for the repair of body defects in low-risk autologous therapeutic strategies. The bioengineering technologies developed for tooth regeneration will make substantial contributions to understand the developmental process and will encourage future organ replacement by regenerative therapies in a wide variety of organs such as the liver, kidney, and heart. The concept of developing tooth banking and preservation of dental stem cells is promising. Further research in the area has the potential to herald a new dawn in effective treatment of notoriously difficult diseases which could prove highly beneficial to mankind in the long run. PMID:28616151

The Neurovascular Properties of Dental Stem Cells and Their Importance in Dental Tissue Engineering

PubMed Central

Ratajczak, Jessica; Bronckaers, Annelies; Dillen, Yörg; Gervois, Pascal; Vangansewinkel, Tim; Driesen, Ronald B.; Wolfs, Esther; Lambrichts, Ivo

2016-01-01

Within the field of tissue engineering, natural tissues are reconstructed by combining growth factors, stem cells, and different biomaterials to serve as a scaffold for novel tissue growth. As adequate vascularization and innervation are essential components for the viability of regenerated tissues, there is a high need for easily accessible stem cells that are capable of supporting these functions. Within the human tooth and its surrounding tissues, different stem cell populations can be distinguished, such as dental pulp stem cells, stem cells from human deciduous teeth, stem cells from the apical papilla, dental follicle stem cells, and periodontal ligament stem cells. Given their straightforward and relatively easy isolation from extracted third molars, dental stem cells (DSCs) have become an attractive source of mesenchymal-like stem cells. Over the past decade, there have been numerous studies supporting the angiogenic, neuroprotective, and neurotrophic effects of the DSC secretome. Together with their ability to differentiate into endothelial cells and neural cell types, this makes DSCs suitable candidates for dental tissue engineering and nerve injury repair. PMID:27688777

The evolution of policy issues in stem cell research: an international survey.

PubMed

Caulfield, Timothy; Rachul, Christen; Zarzeczny, Amy

2012-12-01

Stem cell research remains a tremendously promising yet controversial field of study. It continues to attract considerable public interest and generate discussion and debate. However, while the high profile of this field has endured, the tone and nature of the discourse that drives this profile appears to be changing. In order to get a better sense of how these potential shifts are perceived by individuals directly embedded in the field, we conducted an international internet survey of members of the stem cell research community. Our participants included individuals publishing on both scientific and ethical, legal and social issues topics. We explored the degree to which participants perceived that key policy issues were becoming more or less contentious over time. We queried views regarding the effect of regulatory frameworks on emerging stem cell research technologies and the extent to which participants experience pressure related to clinical translation. We also explored participants' relationships with industry, experience with patents and perceptions regarding the emphasis placed on the potential economic benefits of stem cell research. Our results suggest that while traditional debates such as those surrounding the moral status of the embryo remain, other issues more closely associated with clinical translation and commercialization are perceived as becoming increasingly contentious. This survey provides useful insight into the perspectives of a sample of active researchers working in countries around the world as well as an opportunity to reflect on the likely direction of future stem cell policy debates.

Bursts of CO2 released during freezing offer a new perspective on avoidance of winter embolism in trees.

PubMed

Lintunen, A; Lindfors, L; Kolari, P; Juurola, E; Nikinmaa, E; Hölttä, T

2014-12-01

Woody plants can suffer from winter embolism as gas bubbles are formed in the water-conducting conduits when freezing occurs: gases are not soluble in ice, and the bubbles may expand and fill the conduits with air during thawing. A major assumption usually made in studies of winter embolism formation is that all of the gas dissolved in the xylem sap is trapped within the conduits and forms bubbles during freezing. The current study tested whether this assumption is actually valid, or whether efflux of gases from the stem during freezing reduces the occurrence of embolism. CO2 efflux measurements were conducted during freezing experiments for saplings of three Scots pine (Pinus sylvestris) and three Norway spruce (Picea abies) trees under laboratory conditions, and the magnitudes of the freezing-related bursts of CO2 released from the stems were analysed using a previously published mechanistic model of CO2 production, storage, diffusion and efflux from a tree stem. The freezing-related bursts of CO2 released from a mature Scots pine tree growing in field conditions were also measured and analysed. Substantial freezing-related bursts of CO2 released from the stem were found to occur during both the laboratory experiments and under field conditions. In the laboratory, the fraction of CO2 released from the stem ranged between 27 and 96 % of the total CO2 content within the stem. All gases dissolved in the xylem sap are not trapped within the ice in the stem during freezing, as has previously been assumed, thus adding a new dimension to the understanding of winter embolism formation. The conduit water volume not only determines the volume of bubbles formed during freezing, but also the efficiency of gas efflux out of the conduit during the freezing process. © The Author 2014. Published by Oxford University Press on behalf of the Annals of Botany Company.

Races of Puccinia graminis f. sp. tritici with combined virulence to Sr13 and Sr9e in a field stem rust screening nursery in Ethiopia

USDA-ARS?s Scientific Manuscript database

North American durum lines, selected for resistance to TTKSK and related races of Puccinia graminis f. sp. tritici in Kenya, became highly susceptible in Debre Zeit, Ethiopia, suggesting the presence of stem rust races that were virulent to the TTKSK-effective genes in durum. The objective of this s...

The intersection of discipline and roles: Dr. Pauline Mack's story as an instrumental case study with implications for leadership in science, technology, engineering, and mathematics

NASA Astrophysics Data System (ADS)

Younkin, Winnifred Gail

Widespread concerns currently exist regarding our nation's ability to attract, educate, and retain talented, diverse individuals in STEM fields. These concerns are exacerbated by globalized competition and critical economic conditions. With these issues in mind, this instrumental case study was designed to examine the life story of Dr. Pauline Beery Mack in order to inform secondary and tertiary educational leadership in science, technology, engineering, and mathematics (STEM). Dr. Mack's life story was written from the interpretive view and analyzed through a number of theoretical frameworks appropriate to understanding the complexity of an individual in time and place: the psychobiosocial model, constructivism, creativity, perspective of the field, and the capstone framework of leadership. Data for the construction of Dr. Mack's life story were obtained from Pennsylvania State University Archives, The Women's Collection at Texas Woman's University (TWU), original publications, news media archives, and other sources. Interviews conducted for TWU's archives and interviews conducted by the author provided personal insight into Dr. Mack's life and work. Dr. Mack (1891-1974) lived, learned, created, and taught in a STEM arena that was predominately male. She pursued research with zeal, and was highly adept in attracting jobs, funding, students, and the right people to get the jobs done. Her longitudinal nutrition research, based on a methodology she invented, was innovative in its scope and scale. She served as an advocate for consumers, the undernourished, women, and minorities. Along the way, she changed perceptions of what women could accomplish. Bone density, school lunches, Victory Gardens, flammability, textile industry scandal, and space travel. Dr. Mack with her insatiable need to know was involved in it all. Her multiplicity of roles and contexts yielded a rich and complex life from which to draw implications for educational leadership in secondary and tertiary STEM fields. Implications for educational leadership in secondary and tertiary STEM fields that emerged from this study, relating to both affective and academic spheres, range from STEM valuing, discipline-related expertise, divergence value modeling, and expectations. Among the other implications addressed are levels of thinking, scaffolding for attribute development, and working on the work.

Graft-versus-host disease after radiation therapy in patients who have undergone allogeneic stem cell transplantation: two case reports.

PubMed

Milgrom, Sarah A; Nieto, Yago; Pinnix, Chelsea C; Smith, Grace L; Wogan, Christine F; Rondon, Gabriela; Medeiros, L Jeffrey; Kebriaei, Partow; Dabaja, Bouthaina S

2016-07-28

Patients who undergo allogeneic stem cell transplantation and subsequent radiation therapy uncommonly develop graft-versus-host disease within the irradiated area. We quantified the incidence of this complication, which is a novel contribution to the field. From 2010 to 2014, 1849 patients underwent allogeneic stem cell transplantation, and 41 (2 %) received radiation therapy afterward. Of these, two patients (5 %) developed graft-versus-host disease within the irradiated tissues during or immediately after radiation therapy. The first patient is a 37-year-old white man who had Hodgkin lymphoma; he underwent allogeneic stem cell transplantation from a matched unrelated donor and received radiation therapy for an abdominal and pelvic nodal recurrence. After 28.8 Gy, he developed grade 4 gastrointestinal graft-versus-host disease, refractory to tacrolimus and steroids, but responsive to pentostatin and photopheresis. The other patient is a 24-year-old white man who had acute leukemia; he underwent allogeneic stem cell transplantation from a matched related donor and received craniospinal irradiation for a central nervous system relapse. After 24 cobalt Gy equivalent, he developed severe cutaneous graft-versus-host disease, sharply delineated within the radiation therapy field, which was responsive to tacrolimus and methylprednisolone. We conclude that graft-versus-host disease within irradiated tissues is an uncommon but potentially serious complication that may follow radiation therapy in patients who have undergone allogeneic stem cell transplantation. Clinicians must be aware of this complication and prepared with strategies to mitigate risk. Patients who have undergone allogeneic stem cell transplantation represent a unique population that may offer novel insight into the pathways involved in radiation-related inflammation.

The Potential of Human Stem Cells for the Study and Treatment of Glaucoma

PubMed Central

Chamling, Xitiz; Sluch, Valentin M.; Zack, Donald J.

2016-01-01

Purpose Currently, the only available and approved treatments for glaucoma are various pharmacologic, laser-based, and surgical procedures that lower IOP. Although these treatments can be effective, they are not always sufficient, and they cannot restore vision that has already been lost. The goal of this review is to briefly assess current developments in the application of stem cell biology to the study and treatment of glaucoma and other forms of optic neuropathy. Methods A combined literature review and summary of the glaucoma-related discussion at the 2015 “Sight Restoration Through Stem Cell Therapy” meeting that was sponsored by the Ocular Research Symposia Foundation (ORSF). Results Ongoing advancements in basic and eye-related developmental biology have enabled researchers to direct murine and human stem cells along specific developmental paths and to differentiate them into a variety of ocular cell types of interest. The most advanced of these efforts involve the differentiation of stem cells into retinal pigment epithelial cells, work that has led to the initiation of several human trials. More related to the glaucoma field, there have been recent advances in developing protocols for differentiation of stem cells into trabecular meshwork and retinal ganglion cells. Additionally, efforts are being made to generate stem cell–derived cells that can be used to secrete neuroprotective factors. Conclusions Advancing stem cell technology provides opportunities to improve our understanding of glaucoma-related biology and develop models for drug development, and offers the possibility of cell-based therapies to restore sight to patients who have already lost vision. PMID:27116666

Trends in gender segregation in the choice of science and engineering majors.

PubMed

Mann, Allison; Diprete, Thomas A

2013-11-01

Numerous theories have been put forward for the high and continuing levels of gender segregation in science, technology, engineering, and mathematics (STEM) fields, but research has not systematically examined the extent to which these theories for the gender gap are consistent with actual trends. Using both administrative data and four separate longitudinal studies sponsored by the U.S. Department of Education's National Center for Education Statistics (NCES), we evaluate several prominent explanations for the persisting gender gap in STEM fields related to mathematics performance and background and general life goals, and find that none of them are empirically satisfactory. Instead, we suggest that the structure of majors and their linkages to professional training and careers may combine with gender differences in educational goals to influence the persisting gender gap in STEM fields. An analysis of gendered career aspirations, course-taking patterns, and pathways to medical and law school supports this explanation. Copyright © 2013 Elsevier Inc. All rights reserved.

The relationships among high school STEM learning experiences, expectations, and mathematics and science efficacy and the likelihood of majoring in STEM in college

NASA Astrophysics Data System (ADS)

Sahin, Alpaslan; Ekmekci, Adem; Waxman, Hersh C.

2017-07-01

This study examines college students' science, technology, engineering, and mathematics (STEM) choices as they relate to high school experiences, parent, teacher, and self-expectations, and mathematics and science efficacy. Participants were 2246 graduates of a STEM-focused public Harmony Public Schools in Texas, Harmony Public Schools (HPS). Descriptive analyses indicated that the overall percentage of HPS graduates who chose a STEM major in college was greater than Texas state and national averages. Logistic regression analyses revealed that males and Asian students are more likely to choose a STEM major in college than females and non-Asian students, respectively. Moreover, students whose parents had a college degree in the U.S. are more likely to major in STEM fields than those who did not. Furthermore, males with higher mathematics efficacy and females with higher science efficacy are more likely to choose a STEM major than their counterparts with lower mathematics and science efficacy.

Are stem cells drugs? The regulation of stem cell research and development.

PubMed

Rosen, Michael R

2006-10-31

Stem cell research and its clinical application have become political, social, and medical lightning rods, polarizing opinion among members of the lay community and among medical/scientific professionals. A potpourri of opinion, near-anecdotal observation, and scientifically sound data has sown confusion in ways rarely seen in the medical arts and sciences. A major issue is regulation, with different aspects of stem cell research falling within the purview of different government agencies and local offices. An overarching clearinghouse to review the field and recommend policy is lacking. In the following pages, I touch on the societal framework for regulation, the known and potential risks and benefits of cardiovascular stem cell therapies, whether stem cells should be regulated as drugs or in analogy to drugs, and if there is to be regulation, then by whom. In so doing, I refer to the stem cell literature only as it relates to the discussion of regulation because this is not a review of stem cell research; it is an opinion regarding regulation.

How important is it to my parents? Transmission of STEM academic values: the role of parents' values and practices and children's perceptions of parental influences

NASA Astrophysics Data System (ADS)

Šimunović, Mara; Reić Ercegovac, Ina; Burušić, Josip

2018-06-01

The success of science education in classroom and out-of-school settings can be influenced by parents' behaviours and STEM-related values. The present study investigated pathways in parent-to-child transmission of STEM (science, technology, engineering, mathematics) values by examining at same time parents' values and behaviours, along with their children's perceptions of these parental influences. The study included 1071 students (Mage = 12.15) and the same number of their parents. Path analysis revealed that children's importance value of the STEM school fields was best explained by their perceptions of parental values and behaviours in STEM. On the other hand, parents' self-reported values and behaviours had a weak effect in predicting children's values, which can be explained by inaccurate children's perceptions of their parents. The results suggest that parents more easily convey beliefs about the utility than the attainment value of STEM. Namely, parents' utility value had a larger effect in predicting children's value, partly mediated through children's perception of parents' encouragement of STEM interests. The study highlights the role of children's perceptions of their parents' beliefs and behaviours and the importance of communicating STEM-related values within the family. Practical implications for parents and science educators are discussed.

Women in STEM -- Why We Need All Hands on Deck

ScienceCinema

Wall, Carter

2018-01-26

Carter Wall developed an interest in science at an early age yet struggled to find examples of women scientists and engineers beyond historical figures, like Marie Curie. A lack of relatable role-models didn't stop Carter from pursuing an undergraduate education and career rooted in STEM -- shorthand for science, technology, engineering and mathematics fields. Now, she plays a key role at one of largest solar developers in the Northeast. This is the first in the Energy Department's #WomenInStem video series. At the Energy Department, we're committed to supporting a diverse talent pool of STEM innovators ready to address the challenges and opportunities of our growing clean energy economy

Upper gastrointestinal carcinogenesis: H. pylori and stem cell cross-talk.

PubMed

Pilpilidis, Ioannis; Kountouras, Jannis; Zavos, Christos; Katsinelos, Panagiotis

2011-04-01

Chronic inflammation of the gastric epithelium has been associated with the pathogenesis of gastric cancer, as it was postulated by Corea's model of gastric carcinogenesis. Helicobacter pylori (Hp) regulates this inflammatory process and promotes gastric carcinogenesis through induction of gene mutations and protein modulation. Recent data raise the cancer stem cell hypothesis, which implies a central role of multipotent cancer cells in oncogenesis of various solid tumors. This review provides a synopsis of gastric cancer initiation and promotion through Hp and stem cell signaling pathways. The expanding research field of Hp-related cancer stem cell biology may offer novel implications for future treatment of upper gastrointestinal cancer. Copyright © 2011 Elsevier Inc. All rights reserved.

Women in STEM -- Why We Need All Hands on Deck

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wall, Carter

2014-01-11

Carter Wall developed an interest in science at an early age yet struggled to find examples of women scientists and engineers beyond historical figures, like Marie Curie. A lack of relatable role-models didn't stop Carter from pursuing an undergraduate education and career rooted in STEM -- shorthand for science, technology, engineering and mathematics fields. Now, she plays a key role at one of largest solar developers in the Northeast. This is the first in the Energy Department's #WomenInStem video series. At the Energy Department, we're committed to supporting a diverse talent pool of STEM innovators ready to address the challengesmore » and opportunities of our growing clean energy economy« less

Data sharing in stem cell translational science: policy statement by the International Stem Cell Forum Ethics Working Party.

PubMed

Bredenoord, Annelien L; Mostert, Menno; Isasi, Rosario; Knoppers, Bartha M

2015-01-01

Data and sample sharing constitute a scientific and ethical imperative but need to be conducted in a responsible manner in order to protect individual interests as well as maintain public trust. In 2014, the Global Alliance for Genomics and Health (GA4GH) adopted a common Framework for Responsible Sharing of Genomic and Health-Related Data. The GA4GH Framework is applicable to data sharing in the stem cell field, however, interpretation is required so as to provide guidance for this specific context. In this paper, the International Stem Cell Forum Ethics Working Party discusses those principles that are specific to translational stem cell science, including engagement, data quality and safety, privacy, security and confidentiality, risk-benefit analysis and sustainability.

Mathematically precocious and female: Self-efficacy and STEM course choices among high achieving middle grade students

NASA Astrophysics Data System (ADS)

Burt, Stacey M.

The problem addressed in this project is the lack of mathematically gifted females choosing to pursue advanced science, technology, engineering, and mathematics (STEM) courses in secondary education due to deficiencies in self-efficacy. The purpose of this project was to study the effects of a child-guided robotics program as it relates to the self-efficacy of mathematically gifted 6th grade female students and their future course choices in the advanced STEM content areas. This mixed-model study utilized a STEM attitude survey, artifacts, interviews, field notes, and standardized tests as measurement tools. Significance was found between genders in the treatment group for the standardized science scores, indicating closure in the achievement gap. Research suggests that STEM enrichment is beneficial for mathematically gifted females.

Students' Attitudes and Enrollment Trends in Physics and Engineering

NASA Astrophysics Data System (ADS)

Banjong, Delphine

Science, Technology, Engineering, and Mathematics (STEM) fields are critical for meeting ever-increasing demands in the U.S. for STEM and related skills, and for ensuring the global competitiveness of the United States in technological advancement and scientific innovation. Nonetheless, few U.S. students consider a STEM degree after high school and fewer STEM students end up graduating with a STEM degree. In 2012, the United States ranked 35th in math and 27th in science out of 64 participating countries in the Program for International Student Assessment (PISA), sponsored by the Organization for Economic Cooperation and Development (OECD). Considering the significant role physics and engineering play in technological advancement, this work investigates the attitudes of students and recent enrollment trends in these important subject areas.

STEM Attrition: College Students' Paths into and out of STEM Fields. Statistical Analysis Report. NCES 2014-001

ERIC Educational Resources Information Center

Chen, Xianglei

2013-01-01

Producing sufficient numbers of graduates who are prepared for science, technology, engineering, and mathematics (STEM) occupations has become a national priority in the United States. To attain this goal, some policymakers have targeted reducing STEM attrition in college, arguing that retaining more students in STEM fields in college is a…

Arts-inspired students sync their assets to a nuts and bolts world: A career mentoring pilot progam

NASA Astrophysics Data System (ADS)

Hudson, Lynn

This research examined how students who are arts-inspired feel about their futures in a STEM-based work climate. Science, Technology, Engineering, and Math are the nuts and bolts, and in education today, the only avenue touted for our country and our students' success in this 21st century economy. This can be disconcerting to those interested in other fields, like the arts. This study was guided by the following questions in an effort to understand if our artists and arts-inspired students realize their options and importance in this 21st century climate. The pilot study was designed to help improve the students' perception of their abilities or self-efficacy in the STEM areas by introducing STEM professionals as mentors who designed hands-on activities that simulate work in the STEM fields. Research Questions: 1. Do arts-inspired students have an interest in a STEM career area prior to participating in the career mentoring program? 2. Does participation in a STEM career mentoring program improve student's self-efficacy in STEM fields? 3. Does participation in STEM career mentoring program increase student's interest in pursuing STEM-related careers? Lent, Brown and Hackett's Social Cognitive Career Theory and Daniel Pink's, "A Whole New Mind: Why Left-Brainers Will Rule the Future" were used as the theoretical framework for this study. Seventeen African-American girls who were enrolled in the "I AM COMPLETE" summer program participated in the pilot study. Data was collected from the College Foundation of North Carolina Career Interest Explorer and the STEM Career Interest Survey, which served as a pre and post-test. This pilot offered limited support for the hypothesis, however, career mentoring and opportunities for young people to experience careers, especially in the STEM areas must continue to grow. The role that the arts play in this process is pivotal in galvanizing females and minorities to join these professions. It is the hope of this researcher that the pilot be replicated using a much larger population of students and school communities.

Transport of Asian Aerosols and Trace Gases to North America During the INTEX-B Field Campaign: A Regional Chemical Transport Model Analysis.

NASA Astrophysics Data System (ADS)

Adhikary, B.; Kulkarni, S.; Carmichael, G. R.; Tang, Y.; Dallura, A.; Mena, M.; Streets, D.; Zhang, Q.

2007-12-01

The Intercontinental Chemical Transport Experiment-Phase B (INTEX-B) was conducted over the Pacific Ocean during the 2006 North American spring season. One of the scientific objectives of the INTEX-B field campaign was to quantify the transport and chemical evolution/aging of Asian air pollution into North America. The field campaign deployed multiple experimental platforms such as satellites, aircrafts and surface measurements stations to study the pollution outflow to North America. Three dimensional chemical transport models were used to provide chemical weather forecasts and assist in flight planning during the mission. The Sulfur Transport and dEposition Model (STEM) is a regional chemical transport model developed at the University of Iowa. The STEM model was involved in providing chemical weather forecasts and assist in flight planning during the INTEX-B intensive field campaign. In this study we will report the STEM model performance of aerosols and trace gases in its ability to capture the pollutant plume with experimental observations obtained from the field campaign. The study will then relate the emissions of trace gases and aerosols to atmospheric composition, sources and sinks using the newly developed emissions inventory for the INTEX-B field campaign.

STEAM: A National Study of the Integration of the Arts Into STEM Instruction and its Impact on Student Achievement

NASA Astrophysics Data System (ADS)

Rabalais, Mark E.

The purpose of this study is to examine the relationship between exposure to the arts and performance in Science, Technology, Engineering, and Math (STEM) subjects. STEAM, an integration of arts-based instruction into science and math related fields, is viewed as an alternative to traditional STEM academies. The literature briefly examines the current state of STEM programs and the deficiencies in graduate quality and quantity and the call from employers for a more innovative workforce. Advocates for STEAM argue for arts as a means to improve creativity, collaboration, risk-taking and exploration. Arguments against arts in STEM are grounded in political opinions concerning arts funding and logistical complications of implementing STEAM. However, some schools and STEM programs have embraced the STEAM premise and have begun to integrate arts into the traditional curriculum. The 2009 National Assessment of Educational Progress (NAEP) dataset was utilized to determine a correlation between the number of arts credits earned and mathematics/science achievement. Results from the NAEP dataset indicated a correlation between the amount of arts credits and increased achievement scores in science and math. The same correlation was found when controlling for demographic factors such as gender, race, and socio-economic status (SES). Overall, the arts' greatest impact was on students identified as "at-risk" or underrepresented in STEM fields. Controlling for these variable groups, one can note the quantifiable differences in scores. Overall, findings of the study provide empirical support for the addition of arts in STEM.

Attitudes, Interests, and Perceived Self-Efficacy toward Science of Middle School Minority Female Students: Considerations for Their Low Achievement and Participation in STEM Disciplines

ERIC Educational Resources Information Center

Dowey, Ana Lucrecia

2013-01-01

The under participation of minority females in STEM fields has been a chronic problem in the United States, mainly when it is analyzed through the lens of their relative representation in the population. The results of the first or quantitative phase, of this two phase sequential, mixed method study, revealed academic achievement or performance in…

Understanding the Factors That Support the Use of Active Learning Teaching in STEM Undergraduate Courses: Case Studies in the Field of Geoscience

ERIC Educational Resources Information Center

Iverson, Ellen A. Roscoe

2016-01-01

The purpose of this study was to understand the factors that support the adoption of active learning teaching strategies in undergraduate courses by faculty members, specifically in the STEM disciplines related to geoscience. The focus of the study centered on the context of the department which was identified as a gap in evaluation and…

Characters related to higher starch accumulation in cassava storage roots

PubMed Central

Li, You-Zhi; Zhao, Jian-Yu; Wu, San-Min; Fan, Xian-Wei; Luo, Xing-Lu; Chen, Bao-Shan

2016-01-01

Cassava (Manihot esculenta) is valued mainly for high content starch in its roots. Our understanding of mechanisms promoting high starch accumulation in the roots is, however, still very limited. Two field-grown cassava cultivars, Huanan 124(H124) with low root starch and Fuxuan 01(F01) with high root starch, were characterised comparatively at four main growth stages. Changes in key sugars in the leaves, stems and roots seemed not to be strongly associated with the final amount of starch accumulated in the roots. However, when compared with H124, F01 exhibited a more compact arrangement of xylem vascular bundles in the leaf axils, much less callose around the phloem sieve plates in the stems, higher starch synthesis-related enzymatic activity but lower amylase activity in the roots, more significantly up-regulated expression of related genes, and a much higher stem flow rate (SFR). In conclusion, higher starch accumulation in the roots results from the concurrent effects of powerful stem transport capacity highlighted by higher SFR, high starch synthesis but low starch degradation in the roots, and high expression of sugar transporter genes in the stems. A model of high starch accumulation in cassava roots was therefore proposed and discussed. PMID:26892156

Characters related to higher starch accumulation in cassava storage roots.

PubMed

Li, You-Zhi; Zhao, Jian-Yu; Wu, San-Min; Fan, Xian-Wei; Luo, Xing-Lu; Chen, Bao-Shan

2016-02-19

Cassava (Manihot esculenta) is valued mainly for high content starch in its roots. Our understanding of mechanisms promoting high starch accumulation in the roots is, however, still very limited. Two field-grown cassava cultivars, Huanan 124(H124) with low root starch and Fuxuan 01(F01) with high root starch, were characterised comparatively at four main growth stages. Changes in key sugars in the leaves, stems and roots seemed not to be strongly associated with the final amount of starch accumulated in the roots. However, when compared with H124, F01 exhibited a more compact arrangement of xylem vascular bundles in the leaf axils, much less callose around the phloem sieve plates in the stems, higher starch synthesis-related enzymatic activity but lower amylase activity in the roots, more significantly up-regulated expression of related genes, and a much higher stem flow rate (SFR). In conclusion, higher starch accumulation in the roots results from the concurrent effects of powerful stem transport capacity highlighted by higher SFR, high starch synthesis but low starch degradation in the roots, and high expression of sugar transporter genes in the stems. A model of high starch accumulation in cassava roots was therefore proposed and discussed.

STEM Sell

ERIC Educational Resources Information Center

Pantic, Zorica

2007-01-01

Between 1994 and 2003, employment in science, technology, engineering and math (STEM) fields grew by a remarkable 23 percent, compared with 17 percent in non-STEM fields, according to federal data. The Bureau of Labor Statistics predicts continued strong growth in STEM job openings through 2014, with emphasis on life sciences, environmental…

[Progress in stem cells and regenerative medicine].

PubMed

Wang, Libin; Zhu, He; Hao, Jie; Zhou, Qi

2015-06-01

Stem cells have the ability to differentiate into all types of cells in the body and therefore have great application potential in regenerative medicine, in vitro disease modelling and drug screening. In recent years, stem cell technology has made great progress, and induced pluripotent stem cell technology revolutionizes the whole stem cell field. At the same time, stem cell research in our country has also achieved great progress and becomes an indispensable power in the worldwide stem cell research field. This review mainly focuses on the research progress in stem cells and regenerative medicine in our country since the advent of induced pluripotent stem cell technology, including induced pluripotent stem cells, transdifferentiation, haploid stem cells, and new gene editing tools.

STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells.

PubMed

Kang, Byung-Ho

2016-01-01

Because of the weak penetrating power of electrons, the signal-to-noise ratio of a transmission electron micrograph (TEM) worsens as section thickness increases. This problem is alleviated by the use of the scanning transmission electron microscopy (STEM). Tomography analyses using STEM of thick sections from yeast and mammalian cells are of higher quality than are bright-field (BF) images. In this study, we compared regular BF tomograms and STEM tomograms from 500-nm thick sections from hypertrophied Golgi stacks of alfalfa root cap cells. Due to their thickness and intense heavy metal staining, BF tomograms of the thick sections suffer from poor contrast and high noise levels. We were able to mitigate these drawbacks by using STEM tomography. When we performed STEM tomography of densely stained chloroplasts of Arabidopsis cotyledon, we observed similar improvements relative to BF tomograms. A longer time is required to collect a STEM tilt series than similar BF TEM images, and dynamic autofocusing required for STEM imaging often fails at high tilt angles. Despite these limitations, STEM tomography is a powerful method for analyzing structures of large or dense organelles of plant cells.

Integration of immunological aspects in the European Human Embryonic Stem Cell Registry.

PubMed

Borstlap, Joeri; Kurtz, Andreas

2008-05-01

The immunological properties of stem cells are of increasing importance in regenerative medicine. Immunomodulatory mechanisms seem to play an important role not only with respect to the understanding of underlying mechanisms of autologous versus allogenic therapeutic approaches, but also for endogeneous tissue regeneration. The newly established European human embryonic stem cell registry (hESCreg) offers an international database for the registration, documentation and characterisation of human embryonic stem cells (hESC) and their use. By doing so, hESCreg aims to develop a model procedure for further standardisation efforts in the field of stem cell research and regenerative medicine, and eventually the registry may lead to a repository of therapy-related information. Currently the stem cell characterisation data acquired by the registry are divided into several categories such as cell derivation, culture conditions, genetic constitution, stem cell marker expression and degree of modification. This article describes immunological aspects of stem cell characterisation and explores the layout and relevance of a possible additional section to the hESCreg repository to include immunological characteristics of human embryonic stem cells.

"It's What We Use as a Community": Exploring Students' STEM Characterizations In Two Montessori Elementary Classrooms

NASA Astrophysics Data System (ADS)

Szostkowski, Alaina Hopkins

Integrated science, technology, engineering, and mathematics (STEM) education promises to enhance elementary students' engagement in science and related fields and to cultivate their problem-solving abilities. While STEM has become an increasingly popular reform initiative, it is still developing within the Montessori education community. There is limited research on STEM teaching and learning in Montessori classrooms, particularly from student perspectives. Previous studies suggest productive connections between reform-based pedagogies in mainstream science education and the Montessori method. Greater knowledge of this complementarity, and student perspectives on STEM, may benefit both Montessori and non-Montessori educators. This instrumental case study of two elementary classrooms documented student characterizations of aspects of STEM in the context of integrated STEM instruction over three months in the 2016-2017 school year. Findings show that the Montessori environment played an important role, and that students characterized STEM in inclusive, agentive, connected, helpful, creative, and increasingly critical ways. Implications for teaching and future research offer avenues to envision STEM education more holistically by leveraging the moral and humanistic aspects of Montessori philosophy.

Lipstick and Labcoats: Undergraduate Women's Gender Negotiation in STEM Fields

ERIC Educational Resources Information Center

Goldman, Emily Grey

2012-01-01

Although women have made significant progress in the work force and in education, gender gaps still exist in many industries and occupations, including science, technology, engineering, and math (STEM) fields. This research aimed to understand how undergraduate women negotiate gender within STEM fields, looking specifically at these women's…

Lipstick and Labcoats: Undergraduate Women's Gender Negotiation in STEM Fields

ERIC Educational Resources Information Center

Goldman, Emily Grey

2010-01-01

While women have made significant progress in the work force and in education, gender gaps still exist in many industries and occupations, including science, technology, engineering, and math (STEM) fields. This research aims to understand how undergraduate women negotiate gender within STEM fields, looking specifically at these women's…

Plumbing the STEM Pipeline: Exploring Areas of Influence for Promoting STEM Education

NASA Astrophysics Data System (ADS)

Linger, Matthew

The U.S. has enjoyed several decades of science, technology, engineering and math (STEM) success but that is changing as U.S. students show less interest in advanced learning in STEM fields. Consequently, U.S. students are less scientifically and mathematically literate than past generations, a state that may negatively impact their chances of educational and career success. The reasons for the decline in STEM interest are unclear as many U.S. students still go on to earn STEM degrees and work in STEM fields. Often, these are students who showed a particular capacity for STEM subjects, identified through existing research as earning higher than average SAT scores, especially SAT-math, or high science and math achievement test scores. This study looked at these and other factors as a means to determine what impact they have on U.S. students' interest in pursuing a STEM line of study. The study aimed to determine in what way the U.S. educational system can positively influence high school students toward pursuing a STEM college education. The High School Longitudinal Study of 2009 (HSLS:09) was used as the data source. More than 21,000 students were part of the observations. Factor analysis was used to turn related variables into larger constructs. Constructs and original HSLS:09 variables were analyzed through logistic regression analysis with STATA software. Few high school level instructional and non-instructional interventions were found to have an impact on a student's career choice in 9 th grade of his or her choice of college major in 12th grade. On the contrary, student attitudinal variables were shown to be most influential.

Facilitating Teaching and Learning across STEM Fields

ERIC Educational Resources Information Center

Ejiwale, James A.

2012-01-01

The reformation of the contents for instruction across STEM fields has changed the role of STEM educators from being a "dictator" in the classroom/laboratory to a facilitator of students' activities. More important, this new paradigm and professional orientation for STEM educators is no more limited to delivering instruction intuitively, but with…

Comprehensive Cocurricular Support Promotes Persistence of Community College STEM Students

ERIC Educational Resources Information Center

Shadduck, Peggy

2017-01-01

Despite good career prospects in science, technology, engineering, and mathematics (STEM) fields, persistence of students in STEM fields of study at the community college and transfer to universities to pursue STEM majors is often quite low. Theories of persistence emphasize the importance of engagement, integration, validation, and financial…

Countries with Higher Levels of Gender Equality Show Larger National Sex Differences in Mathematics Anxiety and Relatively Lower Parental Mathematics Valuation for Girls

PubMed Central

2016-01-01

Despite international advancements in gender equality across a variety of societal domains, the underrepresentation of girls and women in Science, Technology, Engineering, and Mathematics (STEM) related fields persists. In this study, we explored the possibility that the sex difference in mathematics anxiety contributes to this disparity. More specifically, we tested a number of predictions from the prominent gender stratification model, which is the leading psychological theory of cross-national patterns of sex differences in mathematics anxiety and performance. To this end, we analyzed data from 761,655 15-year old students across 68 nations who participated in the Programme for International Student Assessment (PISA). Most importantly and contra predictions, we showed that economically developed and more gender equal countries have a lower overall level of mathematics anxiety, and yet a larger national sex difference in mathematics anxiety relative to less developed countries. Further, although relatively more mothers work in STEM fields in more developed countries, these parents valued, on average, mathematical competence more in their sons than their daughters. The proportion of mothers working in STEM was unrelated to sex differences in mathematics anxiety or performance. We propose that the gender stratification model fails to account for these national patterns and that an alternative model is needed. In the discussion, we suggest how an interaction between socio-cultural values and sex-specific psychological traits can better explain these patterns. We also discuss implications for policies aiming to increase girls’ STEM participation. PMID:27100631

Countries with Higher Levels of Gender Equality Show Larger National Sex Differences in Mathematics Anxiety and Relatively Lower Parental Mathematics Valuation for Girls.

PubMed

Stoet, Gijsbert; Bailey, Drew H; Moore, Alex M; Geary, David C

2016-01-01

Despite international advancements in gender equality across a variety of societal domains, the underrepresentation of girls and women in Science, Technology, Engineering, and Mathematics (STEM) related fields persists. In this study, we explored the possibility that the sex difference in mathematics anxiety contributes to this disparity. More specifically, we tested a number of predictions from the prominent gender stratification model, which is the leading psychological theory of cross-national patterns of sex differences in mathematics anxiety and performance. To this end, we analyzed data from 761,655 15-year old students across 68 nations who participated in the Programme for International Student Assessment (PISA). Most importantly and contra predictions, we showed that economically developed and more gender equal countries have a lower overall level of mathematics anxiety, and yet a larger national sex difference in mathematics anxiety relative to less developed countries. Further, although relatively more mothers work in STEM fields in more developed countries, these parents valued, on average, mathematical competence more in their sons than their daughters. The proportion of mothers working in STEM was unrelated to sex differences in mathematics anxiety or performance. We propose that the gender stratification model fails to account for these national patterns and that an alternative model is needed. In the discussion, we suggest how an interaction between socio-cultural values and sex-specific psychological traits can better explain these patterns. We also discuss implications for policies aiming to increase girls' STEM participation.

Direct Visualization of Local Electromagnetic Field Structures by Scanning Transmission Electron Microscopy.

PubMed

Shibata, Naoya; Findlay, Scott D; Matsumoto, Takao; Kohno, Yuji; Seki, Takehito; Sánchez-Santolino, Gabriel; Ikuhara, Yuichi

2017-07-18

The functional properties of materials and devices are critically determined by the electromagnetic field structures formed inside them, especially at nanointerface and surface regions, because such structures are strongly associated with the dynamics of electrons, holes and ions. To understand the fundamental origin of many exotic properties in modern materials and devices, it is essential to directly characterize local electromagnetic field structures at such defect regions, even down to atomic dimensions. In recent years, rapid progress in the development of high-speed area detectors for aberration-corrected scanning transmission electron microscopy (STEM) with sub-angstrom spatial resolution has opened new possibilities to directly image such electromagnetic field structures at very high-resolution. In this Account, we give an overview of our recent development of differential phase contrast (DPC) microscopy for aberration-corrected STEM and its application to many materials problems. In recent years, we have developed segmented-type STEM detectors which divide the detector plane into 16 segments and enable simultaneous imaging of 16 STEM images which are sensitive to the positions and angles of transmitted/scattered electrons on the detector plane. These detectors also have atomic-resolution imaging capability. Using these segmented-type STEM detectors, we show DPC STEM imaging to be a very powerful tool for directly imaging local electromagnetic field structures in materials and devices in real space. For example, DPC STEM can clearly visualize the local electric field variation due to the abrupt potential change across a p-n junction in a GaAs semiconductor, which cannot be observed by normal in-focus bright-field or annular type dark-field STEM imaging modes. DPC STEM is also very effective for imaging magnetic field structures in magnetic materials, such as magnetic domains and skyrmions. Moreover, real-time imaging of electromagnetic field structures can now be realized through very fast data acquisition, processing, and reconstruction algorithms. If we use DPC STEM for atomic-resolution imaging using a sub-angstrom size electron probe, it has been shown that we can directly observe the atomic electric field inside atoms within crystals and even inside single atoms, the field between the atomic nucleus and the surrounding electron cloud, which possesses information about the atomic species, local chemical bonding and charge redistribution between bonded atoms. This possibility may open an alternative way for directly visualizing atoms and nanostructures, that is, seeing atoms as an entity of electromagnetic fields that reflect the intra- and interatomic electronic structures. In this Account, the current status of aberration-corrected DPC STEM is highlighted, along with some applications in real material and device studies.

Involvement of African-American Girls in Science, Technology, Engineering, and Mathematics (STEM) Education

NASA Astrophysics Data System (ADS)

Nkere, Nsidi

A qualitative case study was conducted by examining the perceptions of fifth-grade African American girls about their experiences with science, technology, engineering and mathematics (STEM) education and potential for STEM as a future career. As the United States suffers from waning participation across all demographics in STEM and a high level of underrepresentation of African American women in STEM, the proposed study examined data collected through open-ended interviews with fifth-grade African American girls to explore how their current experiences and perceptions might relate to the underrepresentation of African American women in the STEM fields. Participants were selected from Miracle Elementary School (pseudonym), and consisted of all five students in a small class of high-achieving fifth-grade girls. Data were collected through in-class observations and open-ended interviews, and were analyzed using computer content analysis. The most important key results threaded through the data were related to the importance and role of the teacher, the importance of math to students, the role of experimentation and discovery, and hands-on and personal experience. Future studies are encouraged to utilize longitudinal design to follow students from elementary to university level in an effort to develop and understand the perception, persistence, and experience of all girls in STEM programs.

Personalizing Stem Cell Research and Therapy: The Arduous Road Ahead or Missed Opportunity?

PubMed Central

Patel, S.A.; King, C.C.; Lim, P.K.; Habiba, U.; Dave, M.; Porecha, R.; Rameshwar, P.

2010-01-01

The euphoria of stem cell therapy has diminished, allowing scientists, clinicians and the general public to seriously re-examine how and what types of stem cells would effectively repair damaged tissue, prevent further tissue damage and/or replace lost cells. Importantly, there is a growing recognition that there are substantial person-to-person differences in the outcome of stem cell therapy. Even though the small molecule pharmaceuticals have long remained a primary focus of the personalized medicine research, individualized or targeted use of stem cells to suit a particular individual could help forecast potential failures of the therapy or identify, early on, the individuals who might benefit from stem cell interventions. This would however demand collaboration among several specialties such as pharmacology, immunology, genomics and transplantation medicine. Such transdisciplinary work could also inform how best to achieve efficient and predictable stem cell migration to sites of tissue damage, thereby facilitating tissue repair. This paper discusses the possibility of polarizing immune responses to rationalize and individualize therapy with stem cell interventions, since generalized “one-size-fits-all” therapy is difficult to achieve in the face of the diverse complexities posed by stem cell biology. We also present the challenges to stem cell delivery in the context of the host related factors. Although we focus on the mesenchymal stem cells in this paper, the overarching rationale can be extrapolated to other types of stem cells as well. Hence, the broader purpose of this paper is to initiate a dialogue within the personalized medicine community by expanding the scope of inquiry in the field from pharmaceuticals to stem cells and related cell-based health interventions. PMID:20563265

Supporting Blind Students in STEM Education in Austria.

PubMed

Petz, Andrea; Miesenberger, Klaus

2015-01-01

Equal access to education will foster a knowledge society for all. In particular for the ICT based information society a benchmark has been set to raise the numbers of graduates in science, technology, engineering and mathematics (STEM) study courses by 15% (748.000) per year, asking for increased efforts in Europe (http://ec.europa.eu/education/policy/strategic-framework/index_en.htm). This holds even more true for people with disabilities who a) participate in and graduate from STEM at a much lower number and b) face a much higher unemployment rate, in particular in STEM related fields. This asks for sound and well-founded education - first and foremost in math - for people with disability and here especially for blind people.

Atomic bonding effects in annular dark field scanning transmission electron microscopy. I. Computational predictions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Odlyzko, Michael L.; Mkhoyan, K. Andre, E-mail: mkhoyan@umn.edu; Himmetoglu, Burak

2016-07-15

Annular dark field scanning transmission electron microscopy (ADF-STEM) image simulations were performed for zone-axis-oriented light-element single crystals, using a multislice method adapted to include charge redistribution due to chemical bonding. Examination of these image simulations alongside calculations of the propagation of the focused electron probe reveal that the evolution of the probe intensity with thickness exhibits significant sensitivity to interatomic charge transfer, accounting for observed thickness-dependent bonding sensitivity of contrast in all ADF-STEM imaging conditions. Because changes in image contrast relative to conventional neutral atom simulations scale directly with the net interatomic charge transfer, the strongest effects are seen inmore » crystals with highly polar bonding, while no effects are seen for nonpolar bonding. Although the bonding dependence of ADF-STEM image contrast varies with detector geometry, imaging parameters, and material temperature, these simulations predict the bonding effects to be experimentally measureable.« less

Doctoral Student Attrition in the STEM Fields: An Exploratory Event History Analysis

ERIC Educational Resources Information Center

Lott, Joe L., II; Gardner, Susan; Powers, Daniel A.

2010-01-01

The STEM fields, otherwise known as the Sciences, Technology, Engineering, and Mathematics areas, have become the focus of multiple studies and funding initiatives in recent years. Despite these efforts, lingering concerns exist about who enters, who is retained, and who completes the doctorate in STEM fields. This study utilizes discrete-time…

The under-representation of African American women in the STEM fields within the academy: A historical profile and current perceptions

NASA Astrophysics Data System (ADS)

Howard, Tenisha Senora

This research project seeks to discover the reasons behind the underrepresentation of African American women (AAW) in higher education, particularly in the Science Technology Engineering and Mathematics fields. Why is there underrepresentation of AAW in the STEM fields? Research evidence has demonstrated that AAW face social disparities such as race, gender, and class in the academy. A lack of adequate mentoring and financial resources to support their research efforts are related to these disparities and present fundamental challenges for them. To conduct the inquiry about the barriers AAW have to overcome to achieve success in STEM disciplines, a qualitative research method was used to "attend to social, historical, and temporal context. The findings of these studies are tentatively applied; that is, they may be applicable in diverse situations based on comparability of other contexts" (Mariano, 1995, p. 464). The researcher collected data by conducting in-depth interviews with five participants, using an open-ended conversational format to facilitate the development of trust, rapport, and maximum elicitation of stories from the participants. The results suggest that AAW overcome barriers to successful STEM careers through their family and social ties, mentoring relationships as well as their religious practices.

Three-dimensional bright-field scanning transmission electron microscopy elucidate novel nanostructure in microbial biofilms.

PubMed

Hickey, William J; Shetty, Ameesha R; Massey, Randall J; Toso, Daniel B; Austin, Jotham

2017-01-01

Bacterial biofilms play key roles in environmental and biomedical processes, and understanding their activities requires comprehension of their nanoarchitectural characteristics. Electron microscopy (EM) is an essential tool for nanostructural analysis, but conventional EM methods are limited in that they either provide topographical information alone, or are suitable for imaging only relatively thin (<300 nm) sample volumes. For biofilm investigations, these are significant restrictions. Understanding structural relations between cells requires imaging of a sample volume sufficiently large to encompass multiple cells and the capture of both external and internal details of cell structure. An emerging EM technique with such capabilities is bright-field scanning transmission electron microscopy (BF-STEM) and in the present report BF-STEM was coupled with tomography to elucidate nanostructure in biofilms formed by the polycyclic aromatic hydrocarbon-degrading soil bacterium, Delftia acidovorans Cs1-4. Dual-axis BF-STEM enabled high-resolution 3-D tomographic recontructions (6-10 nm) visualization of thick (1250 and 1500 nm) sections. The 3-D data revealed that novel extracellular structures, termed nanopods, were polymorphic and formed complex networks within cell clusters. BF-STEM tomography enabled visualization of conduits formed by nanopods that could enable intercellular movement of outer membrane vesicles, and thereby enable direct communication between cells. This report is the first to document application of dual-axis BF-STEM tomography to obtain high-resolution 3-D images of novel nanostructures in bacterial biofilms. Future work with dual-axis BF-STEM tomography combined with correlative light electron microscopy may provide deeper insights into physiological functions associated with nanopods as well as other nanostructures. © 2016 The Authors Journal of Microscopy © 2016 Royal Microscopical Society.

Early Experiences and Integration in the Persistence of First-Generation College Students in STEM and Non-STEM Majors

ERIC Educational Resources Information Center

Dika, Sandra L.; D'Amico, Mark M.

2016-01-01

Representation of diverse groups in science, technology, engineering, and mathematics (STEM) fields is a persistent concern in the United States. Although there have been some strides toward more diverse representation, significant problems of underrepresentation remain in particular STEM fields: physical sciences, engineering, math, and computer…

Understanding the STEM Pipeline. Working Paper 125

ERIC Educational Resources Information Center

Sass, Tim R.

2015-01-01

I investigate the determinants of high school completion and college attendance, the likelihood of taking science, technology, engineering or math (STEM) courses in the first year of college and the probability of earning a degree in a STEM field. The focus is on women and minorities, who tend to be underrepresented in STEM fields. Tracking four…

Female STEM majors wanted: The impact of certain factors on choice of a college major

NASA Astrophysics Data System (ADS)

Conrad, Walter Michael

Although females have made significant strides in educational achievements and substantial inroads into academic majors, such as business and medicine, they have made considerably less progress in the science, technology, engineering, and math (STEM) fields. This translates into a smaller number of female graduates prepared to work in the science career fields and results in American industry looking to other countries for its educated workforce. A mixed-methods research design was used to explore and understand the lived experiences and perceptions of faculty members and working STEM professionals in Northern and Central Virginia. Results indicated that although females are attaining STEM degrees and entering STEM fields in record numbers, obstacles such as a challenging STEM curriculum, bias, feelings of insecurity, lack of female role models, and inadequate preparation for the STEM workforce could impede the progress females have made. This research makes recommendations to the academic community and industry which may be used as retention and recruitment strategies for females considering a career in STEM. The ultimate goal is to significantly increase the number of highly skilled female graduates entering STEM fields, leading the U.S. to regain its previous position atop the world in technological innovation and leadership.

Application of low-frequency alternating current electric fields via interdigitated electrodes: effects on cellular viability, cytoplasmic calcium, and osteogenic differentiation of human adipose-derived stem cells.

PubMed

McCullen, Seth D; McQuilling, John P; Grossfeld, Robert M; Lubischer, Jane L; Clarke, Laura I; Loboa, Elizabeth G

2010-12-01

Electric stimulation is known to initiate signaling pathways and provides a technique to enhance osteogenic differentiation of stem and/or progenitor cells. There are a variety of in vitro stimulation devices to apply electric fields to such cells. Herein, we describe and highlight the use of interdigitated electrodes to characterize signaling pathways and the effect of electric fields on the proliferation and osteogenic differentiation of human adipose-derived stem cells (hASCs). The advantage of the interdigitated electrode configuration is that cells can be easily imaged during short-term (acute) stimulation, and this identical configuration can be utilized for long-term (chronic) studies. Acute exposure of hASCs to alternating current (AC) sinusoidal electric fields of 1 Hz induced a dose-dependent increase in cytoplasmic calcium in response to electric field magnitude, as observed by fluorescence microscopy. hASCs that were chronically exposed to AC electric field treatment of 1 V/cm (4 h/day for 14 days, cultured in the osteogenic differentiation medium containing dexamethasone, ascorbic acid, and β-glycerol phosphate) displayed a significant increase in mineral deposition relative to unstimulated controls. This is the first study to evaluate the effects of sinusoidal AC electric fields on hASCs and to demonstrate that acute and chronic electric field exposure can significantly increase intracellular calcium signaling and the deposition of accreted calcium under osteogenic stimulation, respectively.

Translating stem cell therapies: the role of companion animals in regenerative medicine

PubMed Central

Volk, Susan W.; Theoret, Christine

2013-01-01

Veterinarians and veterinary medicine have been integral to the development of stem cell therapies. The contributions of large animal experimental models to the development and refinement of modern hematopoietic stem cell transplantation were noted nearly five decades ago. More recent advances in adult stem cell/regenerative cell therapies continue to expand knowledge of the basic biology and clinical applications of stem cells. A relatively liberal legal and ethical regulation of stem cell research in veterinary medicine has facilitated the development and in some instances clinical translation of a variety of cell-based therapies involving hematopoietic (HSC) and mesenchymal stem cells (MSC) as well as other adult regenerative cells and recently embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In fact, many of the pioneering developments in these fields of stem cell research have been achieved through collaborations of veterinary and human scientists. This review aims to provide an overview of the contribution of large animal veterinary models in advancing stem cell therapies for both human and clinical veterinary applications. Moreover, in the context of the “One Health Initiative”, the role veterinary patients may play in the future evolution of stem cell therapies for both human and animal patients will be explored. PMID:23627495

STEeM- STEM&eTwinning in my school

NASA Astrophysics Data System (ADS)

Nicolaita, Cristina

2017-04-01

My name is Cristina Nicolăiță, I am a teacher of Physics and Computer Science in Gheorghe Magheru School, Caracal, Romania, and a Romanian Scientix&eTwinning ambassador. My poster presents the two eTwinning projects my students are actively involved in, "STEM Club" and "What's the weather like #eTwCitizen2016", with partners mostly from the Mediterranean area. STEM CLUB project focuses on the introductory level STEM activities, as well as awareness of the STEM fields and occupations. This initial step provides standards-based structured inquiry-based and real world problem-based learning, connecting all four of the STEM subjects. Project's basic aims are: 1. increasing student STEM ability, engagement, participation and aspiration 2. increasing teacher capacity and STEM teaching quality 3. supporting STEM education opportunities 4. facilitating effective school partnerships 5. building a strong evidence base 6. improving ICT skills During the second eTwinning project, What's the weather like #eTwCitizen2016", students will investigate weather in our lives, culture and science, in literature (including popular sayings and informative digital literacy), languages, science and Maths( including astronomy, physics, environmental studies) social sciences, religion, art, music, history, psychology (ways of thinking at different weather conditions, feelings related with the weather forecast, careers related to weather, climate change and sustainability).They will use the different intelligences they possess, bringing their contribution to the team work and developing the ability to identify, find, evaluate, and use information effectively.

HPV-Induced Field Cancerisation: Transformation of Adult Tissue Stem Cell Into Cancer Stem Cell.

PubMed

Olivero, Carlotta; Lanfredini, Simone; Borgogna, Cinzia; Gariglio, Marisa; Patel, Girish K

2018-01-01

Field cancerisation was originally described as a basis for multiple head and neck squamous cell carcinoma (HNSCC) and is a pre-malignant phenomenon that is frequently attributable to oncogenic human papillomavirus (HPV) infection. Our work on β-HPV-induced cutaneous squamous cell carcinomas identified a novel Lrig1+ hair follicle junctional zone keratinocyte stem cell population as the basis for field cancerisation. Herein, we describe the ability for HPV to infect adult tissue stem cells in order to establish persistent infection and induce their proliferation and displacement resulting in field cancerisation. By review of the HPV literature, we reveal how this mechanism is conserved as the basis of field cancerisation across many tissues. New insights have identified the capacity for HPV early region genes to dysregulate adult tissue stem cell self-renewal pathways ensuring that the expanded population preserve its stem cell characteristics beyond the stem cell niche. HPV-infected cells acquire additional transforming mutations that can give rise to intraepithelial neoplasia (IEN), from environmental factors such as sunlight or tobacco induced mutations in skin and oral cavity, respectively. With establishment of IEN, HPV viral replication is sacrificed with loss of the episome, and the tissue is predisposed to multiple cancer stem cell-driven carcinomas.

An exploration of the factors that contribute to the success of African American professionals in STEM-related careers

NASA Astrophysics Data System (ADS)

Alexander Nealy, Yolande Kristine

This study examined factors that contribute to the success of African American professionals in STEM careers. Data were collected through a survey from 40 participants and in-depth interviews with eight of them. The survey was used to explore the participants' educational experiences from elementary school through college and on their STEM-related careers, whereas the individual interviews were used to gain insights into their perspectives as STEM professionals. The results of this study indicate that most of these African American STEM professionals attributed their choice of a STEM career to early exposure to and positive experiences in science and mathematics mediated by teachers and/or parents. Furthermore, the positive experiences and success in science and mathematics continued in high school and college, further solidifying their choice of a STEM career. However, for almost half of the participants, attending a HBCU seems to have played an important role in their enjoyment of and success in a STEM major. HBCUs provided them with role models and the necessary support and encouragement to succeed in their pursuit of a STEM degree. The results of this study illustrate the various factors that play a role in preventing leakage in the minority STEM pipeline: K-12 experiences mediated by parents and teachers; support systems in college and the workplace mediated by counselors, professors, peers, and administrators; and policies that facilitate integration and the development of such support systems. This study contributes to the current body of knowledge on minorities in STEM by focusing on what works, instead of focusing on the deficit model and what does not work. It is hoped that these results help validate the efforts of those who work towards a more equitable representation of the STEM fields.

Emerging trends and new developments in regenerative medicine: a scientometric update (2000 - 2014).

PubMed

Chen, Chaomei; Dubin, Rachael; Kim, Meen Chul

2014-09-01

Our previous scientometric review of regenerative medicine provides a snapshot of the fast-growing field up to the end of 2011. The new review identifies emerging trends and new developments appearing in the literature of regenerative medicine based on relevant articles and reviews published between 2000 and the first month of 2014. Multiple datasets of publications relevant to regenerative medicine are constructed through topic search and citation expansion to ensure adequate coverage of the field. Networks of co-cited references representing the literature of regenerative medicine are constructed and visualized based on a combined dataset of 71,393 articles published between 2000 and 2014. Structural and temporal dynamics are identified in terms of most active topical areas and cited references. New developments are identified in terms of newly emerged clusters and research areas. Disciplinary-level patterns are visualized in dual-map overlays. While research in induced pluripotent stem cells remains the most prominent area in the field of regenerative medicine, research related to clinical and therapeutic applications in regenerative medicine has experienced a considerable growth. In addition, clinical and therapeutic developments in regenerative medicine have demonstrated profound connections with the induced pluripotent stem cell research and stem cell research in general. A rapid adaptation of graphene-based nanomaterials in regenerative medicine is evident. Both basic research represented by stem cell research and application-oriented research typically found in tissue engineering are now increasingly integrated in the scientometric landscape of regenerative medicine. Tissue engineering is an interdisciplinary field in its own right. Advances in multiple disciplines such as stem cell research and graphene research have strengthened the connections between tissue engineering and regenerative medicine.

Variation in energy sorghum hybrid TX08001 biomass composition and lignin chemistry during development under irrigated and non-irrigated field conditions

PubMed Central

Olson, Sara N.; Ritter, Kimberley B.; Herb, Dustin W.; Karlen, Steven D.; Lu, Fachuang; Ralph, John; Rooney, William L.; Mullet, John E.

2018-01-01

This study was conducted to document the extent and basis of compositional variation of shoot biomass of the energy Sorghum bicolor hybrid TX08001 during development under field conditions. TX08001 is capable of accumulating ~40 Mg/ha of dry biomass under good growing conditions and this genotype allocates ~80% of its shoot biomass to stems. After 150 days of growth TX08001 stems had a fresh/dry weight ratio of ~3:1 and soluble biomass accounted for ~30% of stem biomass. A panel of diverse energy sorghum genotypes varied ~6-fold in the ratio of stem structural to soluble biomass after 150 days of growth. Near-infrared spectroscopic analysis (NIRS) showed that TX08001 leaves accumulated higher levels of protein, water extractives and ash compared to stems, which have higher sugar, cellulose, and lignin contents. TX08001 stem sucrose content varied during development, whereas the composition of TX08001 stem cell walls, which consisted of ~45–49% cellulose, ~27–30% xylan, and ~15–18% lignin, remained constant after 90 days post emergence until the end of the growing season (180 days). TX08001 and Della stem syringyl (S)/guaiacyl (G) (0.53–0.58) and ferulic acid (FA)/para-coumaric acid (pCA) ratios were similar whereas ratios of pCA/(S+G) differed between these genotypes. Additionally, an analysis of irrigated versus non-irrigated TX08001 revealed that non-irrigated hybrids exhibited a 50% reduction in total cell wall biomass, an ~2-fold increase in stem sugars, and an ~25% increase in water extractives relative to irrigated hybrids. This study provides a baseline of information to help guide further optimization of energy sorghum composition for various end-uses. PMID:29684037

Predicting Undergraduates' Persistence in Science, Technology, Engineering, and Math Fields

ERIC Educational Resources Information Center

Koch, Amanda Joy

2013-01-01

A national shortage of workers in Science, Technology, Engineering, and Math (STEM) occupations has led to efforts to identify why people leave these fields. Lower persistence rates in STEM for females than for males have also led to examinations of features that cause females to leave STEM fields. The current study examines individual- and…

Physics Education Research: A Research Subfield of Physics with Gender Parity

ERIC Educational Resources Information Center

Barthelemy, Ramón S.; Van Dusen, Ramón S.; Henderson, Charles

2015-01-01

Women currently outnumber men in obtaining undergraduate degrees but are underrepresented within STEM fields. However, women's representation varies by STEM field, and even further by STEM subfield. One field that has held a persistent low representation of women is physics. This paper seeks to uncover the truth behind an anecdotal claim that the…

Nurturing Diversity in STEM Fields through Geography: The Past, the Present, and the Future

ERIC Educational Resources Information Center

Oyana, Tonny J.; Garcia, Sonia J.; Haegele, Jennifer A.; Hawthorne, Timothy L.; Morgan, Joe; Young, Nekya Jenise

2015-01-01

To date, there has been a wealth of research on participation in science, technology, engineering, and mathematics (STEM) fields, but most research focuses on the implementation of programs and whether these programs work. Such research can be expanded and enhanced by considering geographic perspectives on participation in the STEM fields and by…

Creating the Workforce of the Future: The STEM Interest and Proficiency Challenge. BHEF Research Brief

ERIC Educational Resources Information Center

Business-Higher Education Forum (NJ1), 2011

2011-01-01

A strong economy requires a highly educated workforce, especially in science, technology, engineering, and math (STEM) fields. In the United States, STEM degree production has stagnated, despite employment projections forecasting a 17% growth in the field over the next decade. Two key criteria influence progression through the STEM education…

Needle in a Haystack? Seeking Causal Evidence about Using STEM Experts to Improve Student Outcomes

ERIC Educational Resources Information Center

Gamse, Beth C.; Martinez, Alina; Bozzi, Laurie

2015-01-01

In today's technological and global society, STEM disciplines are viewed as fundamental to the nation's economic growth and prosperity. Employment opportunities in STEM fields have increased at a faster rate than in non-STEM fields (Government Accounting Office [GAO], 2006). Additionally, many professions--once perceived not to require STEM…

Science identity possibilities: a look into Blackness, masculinities, and economic power relations

NASA Astrophysics Data System (ADS)

Rosa, Katemari

2018-02-01

This forum paper dialogues with Sheron Mark's A bit of both science and economics: a non-traditional STEM identity narrative. In her paper, she discusses the development of a Science, Technology, Engineering, and Mathematics (STEM) identity by a young African American male during an informal STEM for Social Justice Program. Here, the discussion focuses on Black masculinities, identity formation, and the role of science educators in making STEM fields a welcoming place for young Black men. Drawing from Mark's data and discussion, this paper is a dialogue between science identity possibilities in the United States and in Brazil when we look at the intersections of race, gender, and socioeconomic status. Using the shared colonial past of both countries a connection is established to address race relations within science education. The main argument in this paper is that racism can no longer be denied and dismissed by the science education community worldwide and that intersectional approaches are needed to face this issue.

Assessment of Trichogramma japonicum and T. chilonis as Potential Biological Control Agents of Yellow Stem Borer in Rice

PubMed Central

Tang, Rui; Babendreier, Dirk; Zhang, Feng; Kang, Min; Song, Kai; Hou, Mao-Lin

2017-01-01

Two species of Trichogramma wasps were assessed for their effectiveness against yellow stem borer Scirpophaga incertulas. A laboratory cage test with T. japonicum and T. chilonis showed that both species parasitized yellow stem borer egg masses at 60.0% ± 9.13% and 40.7% ± 7.11%, respectively, with egg parasitism rates of 15.8% ± 22.2% for T. japonicum and 2.8% ± 5.0% for T. chilonis. Once the host eggs were parasitized, emergence rates were high for both species (95.7% ± 0.12% for T. japonicum and 100% for T. chilonis). In paddy field trials, the two Trichogramma species were released at three densities (50,000/ha, 100,000/ha and 200,000/ha) in Southwestern China. Egg mass parasitism was 9% ± 7.7% for T. japonicum and 15% ± 14.1% for T. chilonis, and again only a relatively small fraction of eggs was successfully parasitized. No clear conclusion could be drawn on the most efficient release rate as no significant differences were found among the three release rates. A comparison of field-collected T. japonicum with T. japonicum and T. chilonis mass reared on Corcyra cephalonica showed significantly larger body size and ovipositor length in field-collected wasps, suggesting potentially higher effectiveness on yellow stem borer eggs after at least one generation on the target host. Factors contributing to the low field parasitism rates are discussed. PMID:28208706

A Call for Postdoctoral Positions in Mathematics Education

ERIC Educational Resources Information Center

Lockwood, Elise; Knuth, Eric

2014-01-01

In many STEM-related fields, graduating doctoral students are often expected to assume a postdoctoral position as a prerequisite to a faculty position, yet there is no such expectation in mathematics education. This phenomenon is likely due in large part to an abundance of faculty positions; however, it may also result from the field's…

Specific Adoptive Cellular Immunotherapy in Allogeneic Stem Cell Transplantation.

PubMed

Audehm, Stefan; Krackhardt, Angela M

2017-01-01

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) represents a treatment option for a diversity of advanced hematopoietic malignancies providing hope for long-term responses especially due to immunogenic effects associated with the treatment modality. Despite respectable progress in the field, relapses and/or opportunistic infections are major reasons for the high treatment-related mortality. However, a number of novel immunotherapeutic approaches using defined cell populations have been developed to directly target residual malignant cells as well as defined infectious diseases. We here provide an overview of current adoptive cellular immunotherapies in the context of allo-HSCT and close with an outlook on new directions within the field. © 2017 S. Karger GmbH, Freiburg.

Chromatin remodeling and stem cell theory of relativity.

PubMed

Cerny, Jan; Quesenberry, Peter J

2004-10-01

The field of stem cell biology is currently being redefined. Stem cell (hematopoietic and non-hematopoietic) differentiation has been considered hierarchical in nature, but recent data suggest that there is no progenitor/stem cell hierarchy, but rather a reversible continuum. The stem cell (hematopoietic and non-hematopoietic) phenotype, the total differentiation capacity (hematopoietic and non-hematopoietic), gene expression as well as other stem cell functional characteristics (homing, receptor and adhesion molecule expression) vary throughout a cell-cycle transit widely. This seems to be dependent on shifting chromatin and gene expression with cell-cycle transit. The published data on DNA methylation, histone acetylation, and also RNAi, the major regulators of gene expression, conjoins very well and provides an explanation for the major issues of stem cell biology. Those features of stem cells mentioned above can be rather difficult to apprehend when a classical hierarchy biology view is applied, but they become clear and easier to understand once they are correlated with the underlining epigenetic changes. We are entering a new era of stem cell biology the era of "chromatinomics." We are one step closer to the practical use of cellular therapy for degenerative diseases.

Place Based STEM: Leveraging Local Resources to Engage K-12 Teachers in Teaching Integrated STEM and for Addressing the Local STEM Pipeline

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dr. Louis Nadelson; Anne Louise Seifert; Meagan McKinney

Business, industry, parks, nature settings, government infrastructure, and people, can be invaluable resources for connecting STEM curriculum within context which results in conditions ideal for promoting purposeful learning of authentic STEM content. Thus, community-based STEM resources offer ideal context for teaching STEM content. A benefit of focusing teacher attention on these contextual, content aligned resources is that they are in every community; making place-based STEM education a possibility, regardless of the location of STEM teaching and learning. Further, associating STEM teaching and learning with local resources addresses workforce development and the STEM pipeline by exposing students to STEM careers andmore » applications in their local communities. The desire to align STEM teaching and learning with local STEM related resources guided the design of our week-long integrated STEM K-12 teacher professional development (PD) program, i-STEM. We have completed four years of our i-STEM PD program and have made place-based STEM a major emphasis of our curriculum. This report focuses on the data collected in the fourth year of our program. Our week-long i-STEM PD served over 425 educators last summer (2013), providing them with in depth theme-based integrated STEM short courses which were limited to an average of 15 participants and whole group plenary sessions focused around placed based integrated STEM, inquiry, engineering design, standards and practices of Common Core and 21st Century skills. This state wide PD was distributed in five Idaho community colleges and took place over two weeks. The STEM short courses included topics on engineering for sustainability, using engineering to spark interest in STEM, municipal water systems, health, agriculture, food safety, mining, forestry, energy, and others. Integral to these short courses were field trips designed to connect the K-12 educators to the resources in their local communities that could be leveraged for teaching integrated STEM and provide a relevant context for teaching STEM content. Workplace presentations made by place-based STEM experts and provided teachers field trips to place-base STEM industries and business such as manufacturing plants, waste water treatment systems, mines, nature parks, food processing plants, research, hospitals, and laboratory facilities. We researched the 425 participants’ conceptions of place-based STEM prior to and after their taking part in the summer institutes, which included fieldtrips. Our findings revealed substantial increase in our participants’ knowledge, interest, and plans to use place-based resources for teaching integrated STEM. We detail the data analysis and provide a theoretical foundation and justification for the importance of place-based STEM to address the STEM pipeline for the future workforce.« less

Human adipose-derived stem cells: definition, isolation, tissue-engineering applications.

PubMed

Nae, S; Bordeianu, I; Stăncioiu, A T; Antohi, N

2013-01-01

Recent researches have demonstrated that the most effective repair system of the body is represented by stem cells - unspecialized cells, capable of self-renewal through successive mitoses, which have also the ability to transform into different cell types through differentiation. The discovery of adult stem cells represented an important step in regenerative medicine because they no longer raises ethical or legal issues and are more accessible. Only in 2002, stem cells isolated from adipose tissue were described as multipotent stem cells. Adipose tissue stem cells benefits in tissue engineering and regenerative medicine are numerous. Development of adipose tissue engineering techniques offers a great potential in surpassing the existing limits faced by the classical approaches used in plastic and reconstructive surgery. Adipose tissue engineering clinical applications are wide and varied, including reconstructive, corrective and cosmetic procedures. Nowadays, adipose tissue engineering is a fast developing field, both in terms of fundamental researches and medical applications, addressing issues related to current clinical pathology or trauma management of soft tissue injuries in different body locations.

The Effects of an Academic Environment Intervention on Science Identification among Women in STEM

ERIC Educational Resources Information Center

Ramsey, Laura R.; Betz, Diana E.; Sekaquaptewa, Denise

2013-01-01

Academic environments can feel unwelcoming for women in science, technology, engineering, and math (STEM) fields. Two studies examined academic environments of female undergraduates majoring in STEM fields at a university in the United States. In Study 1, we compared women in STEM who are in a welcoming environment to those in a traditional STEM…

Differential Grading: Meta-Analyses of STEM and Non-STEM Fields, Gender, and Institutional Admission Selectivity. ACT Research Report Series 2015 (7)

ERIC Educational Resources Information Center

Westrick, Paul A.

2015-01-01

This study examined the effects of differential grading in science, technology, engineering, and mathematics (STEM) and non-STEM fields over eight consecutive semesters. Using data from 62,122 students at 26 four-year postsecondary institutions, students were subdivided by institutional admission selectivity levels, gender, and student major…

Evidence of correlated evolution and adaptive differentiation of stem and leaf functional traits in the herbaceous genus, Helianthus.

PubMed

Pilote, Alex J; Donovan, Lisa A

2016-12-01

Patterns of plant stem traits are expected to align with a "fast-slow" plant economic spectrum across taxa. Although broad patterns support such tradeoffs in field studies, tests of hypothesized correlated trait evolution and adaptive differentiation are more robust when taxa relatedness and environment are taken into consideration. Here we test for correlated evolution of stem and leaf traits and their adaptive differentiation across environments in the herbaceous genus, Helianthus. Stem and leaf traits of 14 species of Helianthus (28 populations) were assessed in a common garden greenhouse study. Phylogenetically independent contrasts were used to test for evidence of correlated evolution of stem hydraulic and biomechanical properties, correlated evolution of stem and leaf traits, and adaptive differentiation associated with source habitat environments. Among stem traits, there was evidence for correlated evolution of some hydraulic and biomechanical properties, supporting an expected tradeoff between stem theoretical hydraulic efficiency and resistance to bending stress. Population differentiation for suites of stem and leaf traits was found to be consistent with a "fast-slow" resource-use axis for traits related to water transport and use. Associations of population traits with source habitat characteristics supported repeated evolution of a resource-acquisitive "drought-escape" strategy in arid environments. This study provides evidence of correlated evolution of stem and leaf traits consistent with the fast-slow spectrum of trait combinations related to water transport and use along the stem-to-leaf pathway. Correlations of traits with source habitat characteristics further indicate that the correlated evolution is associated, at least in part, with adaptive differentiation of Helianthus populations among native habitats differing in climate. © 2016 Botanical Society of America.

Muscle Stem Cells: A Model System for Adult Stem Cell Biology.

PubMed

Cornelison, Ddw; Perdiguero, Eusebio

2017-01-01

Skeletal muscle stem cells, originally termed satellite cells for their position adjacent to differentiated muscle fibers, are absolutely required for the process of skeletal muscle repair and regeneration. In the last decade, satellite cells have become one of the most studied adult stem cell systems and have emerged as a standard model not only in the field of stem cell-driven tissue regeneration but also in stem cell dysfunction and aging. Here, we provide background in the field and discuss recent advances in our understanding of muscle stem cell function and dysfunction, particularly in the case of aging, and the potential involvement of muscle stem cells in genetic diseases such as the muscular dystrophies.

Larval feeding behavior and ant association in frosted elfin, Callophrys irus (Lycaenidae)

USGS Publications Warehouse

Albanese, G.; Nelson, M.W.; Vickery, P.D.; Sievert, P.R.

2007-01-01

Callophrys irus is a rare and declining lycaenid found in the eastern U.S., inhabiting xeric and open habitats maintained by disturbance. Populations are localized and monophagous. We document a previously undescribed larval feeding behavior in both field and lab reared larvae in which late instar larvae girdled the main stem of the host plant. Girdled stems provide a unique feeding sign that was useful in detecting the presence of larvae in the field. We also observed frequent association of field larvae with several species of ants and provide a list of ant species. We suggest two hypotheses on the potential benefits of stem-girdling to C. irus larvae: 1) Stem girdling provides phloem sap as a larval food source and increases the leaf nutrient concentration, increasing larval growth rates and providing high quality honeydew for attending ants; 2) Stem girdling reduces stem toxicity by inhibiting transport of toxins from roots to the stem.

Impact of electromagnetic fields on stem cells: common mechanisms at the crossroad between adult neurogenesis and osteogenesis

PubMed Central

Leone, Lucia; Podda, Maria Vittoria; Grassi, Claudio

2015-01-01

In the recent years adult neural and mesenchymal stem cells have been intensively investigated as effective resources for repair therapies. In vivo and in vitro studies have provided insights on the molecular mechanisms underlying the neurogenic and osteogenic processes in adulthood. This knowledge appears fundamental for the development of targeted strategies to manipulate stem cells. Here we review recent literature dealing with the effects of electromagnetic fields on stem cell biology that lends support to their use as a promising tool to positively influence the different steps of neurogenic and osteogenic processes. We will focus on recent studies revealing that extremely-low frequency electromagnetic fields enhance adult hippocampal neurogenesis by inducing epigenetic modifications on the regulatory sequences of genes responsible for neural stem cell proliferation and neuronal differentiation. In light of the emerging critical role played by chromatin modifications in maintaining the stemness as well as in regulating stem cell differentiation, we will also attempt to exploit epigenetic changes that can represent common targets for electromagnetic field effects on neurogenic and osteogenic processes. PMID:26124705

From "fixing women" to "institutional transformation": An ADVANCE case study

NASA Astrophysics Data System (ADS)

Yennello, Sherry; Kaunas, Christine

2015-12-01

The United States' position in the global economy requires an influx of women into science, technology, engineering, and mathematics (STEM) fields in order to remain competitive. Despite this, the representation of women in STEM continues to be low. The National Science Foundation's ADVANCE Program addresses this issue by funding projects that aim to increase the representation of women in academic STEM fields through transformation of institutional structures that impede women's progress in academic STEM fields. This paper includes a case study of the Texas A&M University ADVANCE Program.

HEMATOPOIETIC STEM CELL GENE THERAPY: ASSESSING THE RELEVANCE OF PRE-CLINICAL MODELS

PubMed Central

Larochelle, Andre; Dunbar, Cynthia E.

2013-01-01

The modern laboratory mouse has become a central tool for biomedical research with a notable influence in the field of hematopoiesis. Application of retroviral-based gene transfer approaches to mouse hematopoietic stem cells (HSCs) has led to a sophisticated understanding of the hematopoietic hierarchy in this model. However, the assumption that gene transfer methodologies developed in the mouse could be similarly applied to human HSCs for the treatment of human diseases left the field of gene therapy in a decade-long quandary. It is not until more relevant humanized xenograft mouse models and phylogenetically related large animal species were used to optimize gene transfer methodologies that unequivocal clinical successes were achieved. However, the subsequent reporting of severe adverse events in these clinical trials casted doubts on the predictive value of conventional pre-clinical testing, and encouraged the development of new assays for assessing the relative genotoxicity of various vector designs. PMID:24014892

Stem Cell-based Tissue Engineering Approaches for Musculoskeletal Regeneration

PubMed Central

Brown, Patrick T.; Handorf, Andrew M.; Jeon, Won Bae; Li, Wan-Ju

2014-01-01

The field of regenerative medicine and tissue engineering is an ever evolving field that holds promise in treating numerous musculoskeletal diseases and injuries. An important impetus in the development of the field was the discovery and implementation of stem cells. The utilization of mesenchymal stem cells, and later embryonic and induced pluripotent stem cells, opens new arenas for tissue engineering and presents the potential of developing stem cell-based therapies for disease treatment. Multipotent and pluripotent stem cells can produce various lineage tissues, and allow for derivation of a tissue that may be comprised of multiple cell types. As the field grows, the combination of biomaterial scaffolds and bioreactors provides methods to create an environment for stem cells that better represent their microenvironment for new tissue formation. As technologies for the fabrication of biomaterial scaffolds advance, the ability of scaffolds to modulate stem cell behavior advances as well. The composition of scaffolds could be of natural or synthetic materials and could be tailored to enhance cell self-renewal and/or direct cell fates. In addition to biomaterial scaffolds, studies of tissue development and cellular microenvironments have determined other factors, such as growth factors and oxygen tension, that are crucial to the regulation of stem cell activity. The overarching goal of stem cell-based tissue engineering research is to precisely control differentiation of stem cells in culture. In this article, we review current developments in tissue engineering, focusing on several stem cell sources, induction factors including growth factors, oxygen tension, biomaterials, and mechanical stimulation, and the internal and external regulatory mechanisms that govern proliferation and differentiation. PMID:23432679

Basic Science and Clinical Application of Stem Cells in Veterinary Medicine

NASA Astrophysics Data System (ADS)

Ribitsch, I.; Burk, J.; Delling, U.; Geißler, C.; Gittel, C.; Jülke, H.; Brehm, W.

Stem cells play an important role in veterinary medicine in different ways. Currently several stem cell therapies for animal patients are being developed and some, like the treatment of equine tendinopathies with mesenchymal stem cells (MSCs), have already successfully entered the market. Moreover, animal models are widely used to study the properties and potential of stem cells for possible future applications in human medicine. Therefore, in the young and emerging field of stem cell research, human and veterinary medicine are intrinsically tied to one another. Many of the pioneering innovations in the field of stem cell research are achieved by cooperating teams of human and veterinary medical scientists.

Sino-Canadian collaborations in stem cell research: a scientometric analysis.

PubMed

Ali-Khan, Sarah E; Ray, Monali; McMahon, Dominique S; Thorsteinsdóttir, Halla

2013-01-01

International collaboration (IC) is essential for the advance of stem cell research, a field characterized by marked asymmetries in knowledge and capacity between nations. China is emerging as a global leader in the stem cell field. However, knowledge on the extent and characteristics of IC in stem cell science, particularly China's collaboration with developed economies, is lacking. We provide a scientometric analysis of the China-Canada collaboration in stem cell research, placing this in the context of other leading producers in the field. We analyze stem cell research published from 2006 to 2010 from the Scopus database, using co-authored papers as a proxy for collaboration. We examine IC levels, collaboration preferences, scientific impact, the collaborating institutions in China and Canada, areas of mutual interest, and funding sources. Our analysis shows rapid global expansion of the field with 48% increase in papers from 2006 to 2010. China now ranks second globally after the United States. China has the lowest IC rate of countries examined, while Canada has one of the highest. China-Canada collaboration is rising steadily, more than doubling during 2006-2010. China-Canada collaboration enhances impact compared to papers authored solely by China-based researchers This difference remained significant even when comparing only papers published in English. While China is increasingly courted in IC by developed countries as a partner in stem cell research, it is clear that it has reached its status in the field largely through domestic publications. Nevertheless, IC enhances the impact of stem cell research in China, and in the field in general. This study establishes an objective baseline for comparison with future studies, setting the stage for in-depth exploration of the dynamics and genesis of IC in stem cell research.

Sino-Canadian Collaborations in Stem Cell Research: A Scientometric Analysis

PubMed Central

Ali-Khan, Sarah E.; Ray, Monali; McMahon, Dominique S.; Thorsteinsdóttir, Halla

2013-01-01

Background International collaboration (IC) is essential for the advance of stem cell research, a field characterized by marked asymmetries in knowledge and capacity between nations. China is emerging as a global leader in the stem cell field. However, knowledge on the extent and characteristics of IC in stem cell science, particularly China’s collaboration with developed economies, is lacking. Methods and Findings We provide a scientometric analysis of the China–Canada collaboration in stem cell research, placing this in the context of other leading producers in the field. We analyze stem cell research published from 2006 to 2010 from the Scopus database, using co-authored papers as a proxy for collaboration. We examine IC levels, collaboration preferences, scientific impact, the collaborating institutions in China and Canada, areas of mutual interest, and funding sources. Our analysis shows rapid global expansion of the field with 48% increase in papers from 2006 to 2010. China now ranks second globally after the United States. China has the lowest IC rate of countries examined, while Canada has one of the highest. China–Canada collaboration is rising steadily, more than doubling during 2006–2010. China–Canada collaboration enhances impact compared to papers authored solely by China-based researchers This difference remained significant even when comparing only papers published in English. Conclusions While China is increasingly courted in IC by developed countries as a partner in stem cell research, it is clear that it has reached its status in the field largely through domestic publications. Nevertheless, IC enhances the impact of stem cell research in China, and in the field in general. This study establishes an objective baseline for comparison with future studies, setting the stage for in-depth exploration of the dynamics and genesis of IC in stem cell research. PMID:23468927

Tree water relations can trigger monoterpene emissions from Scots pine stems during spring recovery

NASA Astrophysics Data System (ADS)

Vanhatalo, A.; Chan, T.; Aalto, J.; Korhonen, J. F.; Kolari, P.; Hölttä, T.; Nikinmaa, E.; Bäck, J.

2015-09-01

Tree canopies are known to emit large amounts of VOCs (volatile organic compounds) such as monoterpenes into the surrounding air. High VOC emission rates from boreal forests have been observed during the transition from winter to summer activity. The most important sources of these are considered to be the green foliage, understory vegetation and soil organisms, but emissions from the living stand woody compartments have so far not been quantified. We analyzed whether the non-foliar components could partially explain the springtime high emission rates. We measured the monoterpene emissions from Scots pine (Pinus sylvestris L.) stem and shoots during the dehardening phase of trees in field conditions in two consecutive springs. We observed a large, transient monoterpene burst from the stem, while the shoot monoterpene emissions remained low. The burst lasted about 12 h. Simultaneously, an unusual nighttime sap flow and a non-systematic diurnal pattern of tree diameter were detected. Hence, we suggest that the monoterpene burst was a consequence of the recovery of the stem from wintertime, and likely related to the refilling of embolized tracheids and/or phenological changes in the living cells of the stem. This indicates that the dominant processes and environmental drivers triggering the monoterpene emissions are different between the stem and the foliage.

Concise Review: Human Dermis as an Autologous Source of Stem Cells for Tissue Engineering and Regenerative Medicine.

PubMed

Vapniarsky, Natalia; Arzi, Boaz; Hu, Jerry C; Nolta, Jan A; Athanasiou, Kyriacos A

2015-10-01

The exciting potential for regenerating organs from autologous stem cells is on the near horizon, and adult dermis stem cells (DSCs) are particularly appealing because of the ease and relative minimal invasiveness of skin collection. A substantial number of reports have described DSCs and their potential for regenerating tissues from mesenchymal, ectodermal, and endodermal lineages; however, the exact niches of these stem cells in various skin types and their antigenic surface makeup are not yet clearly defined. The multilineage potential of DSCs appears to be similar, despite great variability in isolation and in vitro propagation methods. Despite this great potential, only limited amounts of tissues and clinical applications for organ regeneration have been developed from DSCs. This review summarizes the literature on DSCs regarding their niches and the specific markers they express. The concept of the niches and the differentiation capacity of cells residing in them along particular lineages is discussed. Furthermore, the advantages and disadvantages of widely used methods to demonstrate lineage differentiation are considered. In addition, safety considerations and the most recent advancements in the field of tissue engineering and regeneration using DSCs are discussed. This review concludes with thoughts on how to prospectively approach engineering of tissues and organ regeneration using DSCs. Our expectation is that implementation of the major points highlighted in this review will lead to major advancements in the fields of regenerative medicine and tissue engineering. Autologous dermis-derived stem cells are generating great excitement and efforts in the field of regenerative medicine and tissue engineering. The substantial impact of this review lies in its critical coverage of the available literature and in providing insight regarding niches, characteristics, and isolation methods of stem cells derived from the human dermis. Furthermore, it provides analysis of the current state-of-the-art regenerative approaches using human-derived dermal stem cells, with consideration of current guidelines, to assist translation toward therapeutic use. ©AlphaMed Press.

Concise Review: Human Dermis as an Autologous Source of Stem Cells for Tissue Engineering and Regenerative Medicine

PubMed Central

Vapniarsky, Natalia; Arzi, Boaz; Hu, Jerry C.; Nolta, Jan A.

2015-01-01

The exciting potential for regenerating organs from autologous stem cells is on the near horizon, and adult dermis stem cells (DSCs) are particularly appealing because of the ease and relative minimal invasiveness of skin collection. A substantial number of reports have described DSCs and their potential for regenerating tissues from mesenchymal, ectodermal, and endodermal lineages; however, the exact niches of these stem cells in various skin types and their antigenic surface makeup are not yet clearly defined. The multilineage potential of DSCs appears to be similar, despite great variability in isolation and in vitro propagation methods. Despite this great potential, only limited amounts of tissues and clinical applications for organ regeneration have been developed from DSCs. This review summarizes the literature on DSCs regarding their niches and the specific markers they express. The concept of the niches and the differentiation capacity of cells residing in them along particular lineages is discussed. Furthermore, the advantages and disadvantages of widely used methods to demonstrate lineage differentiation are considered. In addition, safety considerations and the most recent advancements in the field of tissue engineering and regeneration using DSCs are discussed. This review concludes with thoughts on how to prospectively approach engineering of tissues and organ regeneration using DSCs. Our expectation is that implementation of the major points highlighted in this review will lead to major advancements in the fields of regenerative medicine and tissue engineering. Significance Autologous dermis-derived stem cells are generating great excitement and efforts in the field of regenerative medicine and tissue engineering. The substantial impact of this review lies in its critical coverage of the available literature and in providing insight regarding niches, characteristics, and isolation methods of stem cells derived from the human dermis. Furthermore, it provides analysis of the current state-of-the-art regenerative approaches using human-derived dermal stem cells, with consideration of current guidelines, to assist translation toward therapeutic use. PMID:26253713

Females and STEM: Determining the K-12 Experiences that Influenced Women to Pursue STEM Fields

NASA Astrophysics Data System (ADS)

Petersen, Anne Marie

In the United States, careers in the fields of Science, Technology, Engineering, and Mathematics (STEM) are increasing yet there are not enough trained personnel to meet this demand. In addition, of those that seek to pursue STEM fields in the United States, only 26% are female. In order to increase the number of women seeking STEM based bachelor's degrees, K-12 education must provide a foundation that prepares students for entry into these fields. The purpose of this phenomenological study was to determine the perceived K-12 experiences that influenced females to pursue a STEM field. Twelve college juniors or seniors seeking a degree in Biology, Mathematics, or Physics were interviewed concerning their K-12 experiences. These interviews were analyzed and six themes emerged. Teacher passion and classroom characteristics such as incorporating challenging activities played a significant role in the females' decisions to enter STEM fields. Extra-curricular activities such as volunteer and mentor opportunities and the females' need to benefit others also influenced females in their career choice. Both the formal (within the school) and informal (outside of the traditional classroom) pipeline opportunities that these students encountered helped develop a sense of self-efficacy in science and mathematics; this self-efficacy enabled them to persist in pursuing these career fields. Several participants cited barriers that they encountered in K-12 education, but these barriers were primarily internal as they struggled with overcoming self-imposed obstacles in learning and being competitive in the mathematics and science classrooms. The experiences from these female students can be used by K-12 educators to prepare and encourage current female students to enter STEM occupations.

Parasitism of the wheat stem sawfly (Hymenoptera: Cephidae) by Bracon cephi and B. liflogaster (Hymenoptera: Braconidae) in wheat fields bordering tilled and untilled fallow in Montana

Treesearch

J. B. Runyon; W. L. Morrill; D. K. Weaver; P. R. Miller

2002-01-01

We evaluated wheat stem sawfly, Cephus cinctus Norton, parasitism, infestation, and sawfly-cut stems in wheat fields bordering intensely tilled (no visible stubble residue), minimally tilled (>75% stubble residue visible), and untilled (chemical fallow, herbicide fallow management) summer fallow fields in north-central and south-central Montana....

Where will the stem cells lead us? Prospects for dentistry in the 21st century

PubMed Central

Sreenivas, S. Durga; Rao, Akula Sreenivasa; Satyavani, S. Sri; Reddy, Bavigadda Harish; Vasudevan, Sanjay

2011-01-01

It is dentists’ dream to achieve bone repair with predictability, but without donor site morbidity as well as reconstruction of injured or pathologically damaged complex dental structures, however, this will no longer be a dream as these are being made into a reality using stem cell science. Stem cell science is clearly an intriguing and promising area of science. Stem cells have been isolated from a variety of embryonic and adult tissues. Dental stem cells are multipotent mesenchymal stem cells (MSCs) brought new enthusiasm among the researchers because of their easy accessibility, high quality and they don’t pose the same ethical concerns and controversy in comparison with embryonic stem cells. This review article provides brief insights about stem cell basics, the state of art in human dental stem cell research and its possible impact on future dentistry. Even though most of these modalities are still in infancy, it is evident that the 21st century dentist is going to play a critical role in the field of medicine. The aim of this article is to bring awareness among the dentists about the huge potential associated with the use of stem cells in a clinical setting, as well as proper understanding of related problems. PMID:22028504

Psychosocial Pathways to STEM Engagement among Graduate Students in the Life Sciences

PubMed Central

Clark, Sheri L.; Dyar, Christina; Maung, Nina; London, Bonita

2016-01-01

Despite growing diversity among life sciences professionals, members of historically underrepresented groups (e.g., women) continue to encounter barriers to academic and career advancement, such as subtle messages and stereotypes that signal low value for women, and fewer opportunities for quality mentoring relationships. These barriers reinforce the stereotype that women’s gender is incompatible with their science, technology, engineering, and mathematics (STEM) field, and can interfere with their sense of belonging and self-efficacy within STEM. The present work expands this literature in two ways, by 1) focusing on a distinct period in women’s careers that has been relatively understudied, but represents a critical period when career decisions are made, that is, graduate school; and 2) highlighting the buffering effect of one critical mechanism against barriers to STEM persistence, that is, perceived support from advisors. Results of the present study show that perceived support from one’s advisor may promote STEM engagement among women by predicting greater gender–STEM identity compatibility, which in turn predicts greater STEM importance among women (but not men). STEM importance further predicts higher sense of belonging in STEM for both men and women and increased STEM self-efficacy for women. Finally, we describe the implications of this work for educational policy. PMID:27562961

Day at Goddard

NASA Astrophysics Data System (ADS)

Wawro, Martha; Van Norden, Wendy

2013-03-01

Day at Goddard is an all day event for high school students that the SDO EPO team has been running for 5 years now. During the event, students are given a tour of the integration and testing facilities, shown science on a sphere, participate in a meet and greet with scientists and engineers and participate in a hands-on lab activity. The purpose of these field trips is to increase the students' interest in STEM subjects, expose them to STEM-related careers and increase their awareness of the research that NASA conducts.

Students Who Demonstrate Strong Talent and Interest in STEM Are Initially Attracted to STEM through Extracurricular Experiences

PubMed Central

Frankenfeld, Cara L.; Bases, Jessica; Espina, Virginia; Liotta, Lance A.

2014-01-01

What early experiences attract students to pursue an education and career in science, technology, engineering, and mathematics (STEM)? Does hands-on research influence them to persevere and complete a major course of academic study in STEM? We evaluated survey responses from 149 high school and undergraduate students who gained hands-on research experience in the 2007–2013 Aspiring Scientists Summer Internship Programs (ASSIP) at George Mason University. Participants demonstrated their strong interest in STEM by volunteering to participate in ASSIP and completing 300 h of summer research. The survey queried extracurricular experiences, classroom factors, and hands-on projects that first cultivated students’ interest in the STEM fields, and separately evaluated experiences that sustained their interest in pursuing a STEM degree. The majority of students (65.5%, p < 0.0001) reported extracurricular encounters, such as the influence of a relative or family member and childhood experiences, as the most significant factors that initially ignited their interest in STEM, while hands-on lab work was stated as sustaining their interest in STEM (92.6%). Based on these findings collected from a cohort of students who demonstrated a strong talent and interest in STEM, community-based programs that create awareness about STEM for both children and their family members may be key components for igniting long-term academic interest in STEM. PMID:25452491

Persistence in STEM: An investigation of the relationship between high school experiences in science and mathematics and college degree completion in STEM fields

NASA Astrophysics Data System (ADS)

Maltese, Adam V.

While the number of Bachelor's degrees awarded annually has nearly tripled over the past 40 years (NSF, 2008), the same cannot be said for degrees in the STEM (science, technology, engineering and mathematics) fields. The Bureau of Labor Statistics projects that by the year 2014 the combination of new positions and retirements will lead to 2 million job openings in STEM (BLS, 2005). Thus, the research questions I sought to answer with this study were: (1)What are the most common enrollment patterns for students who enter into and exit from the STEM pipeline during high school and college? (2) Controlling for differences in student background and early interest in STEM careers, what are the high school science and mathematics classroom experiences that characterize student completion of a college major in STEM? Using data from NELS:88 I analyzed descriptive statistics and completed logistic regressions to gain an understanding of factors related to student persistence in STEM. Approximately 4700 students with transcript records and who participated in all survey rounds were included in the analyses. The results of the descriptive analysis demonstrated that most students who went on to complete majors in STEM completed at least three or four years of STEM courses during high school, and enrolled in advanced high school mathematics and science courses at higher rates. At almost every pipeline checkpoint indicators of the level of coursework and achievement were significant in predicting student completion of a STEM degree. The results also support previous research that showed demographic variables have little effect on persistence once the sample is limited to those who have the intrinsic ability and desire to complete a college degree. The most significant finding is that measures of student interest and engagement in science and mathematics were significant in predicting completion of a STEM degree, above and beyond the effects of course enrollment and performance. A final analysis, which involved the comparison of descriptive statistics for students who switched into and out of the STEM pipeline during high school, suggested that attitudes toward mathematics and science play a major role in choices regarding pipeline persistence.

Social class and the STEM career pipeline an ethnographic investigation of opportunity structures in a high-poverty versus affluent high school

NASA Astrophysics Data System (ADS)

Nikischer, Andrea B.

This research investigates science, technology, engineering and mathematics (STEM) high school opportunity structures, including student experiences with math and science course sequences and progress, college guidance and counseling, and STEM extracurricular activities (Weis and Eisenhart, 2009), specifically related to STEM fields and career and college choice, for top-performing math and science students. Differences in these structures and processes as they play out in two representative high schools that vary by social class and racial/ethnic makeup are examined. This comparative ethnography includes 36 school and classroom observations, 56 semi-structured individual interviews, and a review of relevant documents, all gathered during the focal students' junior year of high school. Three data chapters are presented, discussing three distinct, yet interconnected themes. In the first, I examine the ways in which chronic attendance problems and classroom distractions negatively impact math and science instruction time and lead to an instruction (time) deficit. In the second, I compare the math and science course and extra-curricular offerings at each school, and discuss the significant differences between sites regarding available STEM exposure and experience, also known as "STEM educational dose" (Wai, et al., 2010). In the third, I investigate available guidance counseling services and STEM and college-linking at each site. Perceived failures in the counseling services available are discussed. This dissertation is grounded in the literature on differences in academic achievement based on school setting, the nature/distribution of knowledge based on social class, and STEM opportunity structures. The concepts of "social capital" and "STEM capital" are engaged throughout. Ultimately, I argue through this dissertation that segregation by race, and most importantly social class, both between and within districts, damages the STEM pipeline for high-performing math and science students located in high-poverty, low-performing schools. I further argue that both federal and state accountability-based school reform efforts are failing to improve outcomes for students with proficiency and interest in STEM learning and STEM fields, and in fact, these reforms are harming top performing students and high school STEM opportunity structures. Recommendations for changes in policy and practice, and for further research, are provided.

Current applications of human pluripotent stem cells: possibilities and challenges.

PubMed

Ho, Pai-Jiun; Yen, Men-Luh; Yet, Shaw-Fang; Yen, B Linju

2012-01-01

Stem cells are self-renewable cells with the differentiation capacity to develop into somatic cells with biological functions. This ability to sustain a renewable source of multi- and/or pluripotential differentiation has brought new hope to the field of regenerative medicine in terms of cell therapy and tissue engineering. Moreover, stem cells are invaluable tools as in vitro models for studying diverse fields, from basic scientific questions such as developmental processes and lineage commitment, to practical application including drug screening and testing. The stem cells with widest differentiation potential are pluripotent stem cells (PSCs), which are rare cells with the ability to generate somatic cells from all three germ layers. PSCs are considered the most optimal choice for therapeutic potential of stem cells, bringing new impetus to the field of regenerative medicine. In this article, we discuss the therapeutic potential of human PSCs (hPSCs) including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), reviewing the current preclinical and clinical data using these stem cells. We describe the classification of different sources of hPSCs, ongoing research, and currently encountered clinical obstacles of these novel and versatile human stem cells.

The king is dead, long live the king: entering a new era of stem cell research and clinical development.

PubMed

Ichim, Thomas; Riordan, Neil H; Stroncek, David F

2011-12-20

In mid November the biopharma industry was shocked by the announcement from Geron that they were ending work on embryonic stem cell research and therapy. For more than 10 years the public image of all stem cell research has been equated with embryonic stem cells. Unfortunately, a fundamentally important medical and financial fact was being ignored: embryonic stem cell therapy is extremely immature. In parallel to efforts in embryonic stem cell research and development, scientists and physicians in the field of adult stem cells realized that the natural role of adult stem cells in the body is to promote healing and to act like endogenous "repair cells" and, as a result, numerous companies have entered the field of adult stem cell therapy with the goal of expanding numbers of adult stem cells for administration to patients with various conditions. In contrast to embryonic stem cells, which are extremely expensive and potentially dangerous, adult cell cells are inexpensive and have an excellent safety record when used in humans. Many studies are now showing that adult stem cells are practical, patient-applicable, therapeutics that are very close to being available for incorporation into the practice of medicine. These events signal the entrance of the field of stem cells into a new era: an era where hype and misinformation no longer triumph over economic and medical realities.

Cool Astronomy: Education and Public Outreach for the WISE mission

NASA Astrophysics Data System (ADS)

Mendez, Bryan J.

2011-01-01

The Education and Public Outreach (E/PO) program of the Wide-field Infrared Survey Explorer (WISE) aims to educate and engage students, teachers, and the general public in the endeavor of science. We bring a collection of accomplished professionals in formal and informal astronomy education from around the nation to create learning materials and experiences that appeal to broad audiences. Our E/PO program trains teachers in science, technology, engineering, and mathematics (STEM) topics related to WISE; creates standards-based classroom resources and lessons using WISE data and WISE-related STEM topics; develops interactive programming for museums and science centers; and inspires the public with WISE science and images.

Facilitating Collaboration across Science, Technology, Engineering & Mathematics (STEM) Fields in Program Development

ERIC Educational Resources Information Center

Ejiwale, James A.

2014-01-01

Collaboration plays a major role in interdisciplinary activities among Science, Technology, Engineering & Mathematics (STEM) disciplines or fields. It also affects the relationships among cluster members on the management team. Although effective collaboration does not guarantee success among STEM disciplines, its absence usually assures…

Transforming attitudes and lives: Liberating African-American elementary and middle school students in out-of-school time STEM education

NASA Astrophysics Data System (ADS)

Smith, Charisse F.

Statistically, African-Americans, women, and the disabled are underrepresented in the fields of Science, Technology, Engineering, and Math (STEM). Historically, these underrepresented students, are described as being unrecognized and underdeveloped in the American STEM circuit. Many experience deficient and inadequate educational resources, are not encouraged to pursue STEM education and careers, and are confronted with copious obstructions. In this quantitative study, the researcher collected pretest and posttest survey data from a group of 4th, 5th, and 6th-grade African-American students in Title I funded schools. The reseacher used quantitative analysis to determine any significant differences in the science related attitudes between and within groups who participated in Out of School-Time Science, Technology, Engineering, and Mathematics programs and those who did not. Results revealed no significant differences in the science related attitudes between the groups of the students who participated in the Out of School Time-Science, Technology, Engineering, and Mathematics programs and those who did not. Results also revealed no significant differences in the science related attitudes within the groups of students who participated in the Out of School Time-Science, Technology, Engineering, and Mathematics programs and those who did not.

Effects of Participation in a STEM Camp on STEM Attitudes and Anticipated Career Choices of Middle School Girls: A Mixed Methods Study

NASA Astrophysics Data System (ADS)

Kager, Elisabeth

Middle school is a critical time for the development of girls' attitudes toward science, technology, engineering, and mathematics (STEM). Existing research has indicated declining positive attitudes toward these fields among girls throughout adolescence. This study investigated how, to what extent, and for whom participation in a summer STEM Camp at a Midwestern college in the United States affected the STEM attitudes and career aspirations of 23 female participants, ages 10-14 years. Using a concurrent triangulation design, the researcher collected pre- and post-questionnaire data (N = 20), interviewed participants (N = 9), read journal entries (N = 22), and wrote field notes. The researcher adapted the Fennema-Sherman Attitude Scales (FSAS) to measure five of the original nine attitude scales concerning STEM: Male Domain, Confidence, Usefulness, Success, and Motivation. In addition to these standardized, Likert-type scale questions, the questionnaire included demographic items to gauge participants' anticipated career choices and the level of STEM motivation (e.g., extracurricular activities and guardians' STEM involvement). The interview questions elicited information about the participants' Camp experiences and the Camp's influence on participants' attitudes and career aspirations. The journal prompts provoked participants to think about their perceptions of, and relationship with, science and mathematics as well as how supportive their parents and peers had been regarding these two fields. Participants' incoming STEM attitudes were positive. Accordingly, there was no statistically significant difference between pre- and post-scores of attitudes toward STEM. Nevertheless, qualitative results showed that the Camp did strengthen participants' positive attitudes through enthusiastic instructors, STEM-motivated peers, and hands-on activities that allowed for creative freedom. Participating in the STEM Camp challenged participants' prior career aspirations by introducing them to new STEM fields and careers to be considered. Meta-inference showed that participating in the Camp had a positive effect on the participants' attitude toward, motivation in, and awareness of STEM. The results suggest that camp instructors should collaboratively plan inquiry-based activities to maximize interrelatedness of STEM fields and to ensure cognitively challenging tasks for participants and that classroom teachers should adopt interactive, hands-on, and collaborative teaching strategies to boost the positive STEM attitudes of girls.

Chemical genetics and its potential in cardiac stem cell therapy

PubMed Central

Vieira, Joaquim M; Riley, Paul R

2013-01-01

Over the last decade or so, intensive research in cardiac stem cell biology has led to significant discoveries towards a potential therapy for cardiovascular disease; the main cause of morbidity and mortality in humans. The major goal within the field of cardiovascular regenerative medicine is to replace lost or damaged cardiac muscle and coronaries following ischaemic disease. At present, de novo cardiomyocytes can be generated either in vitro, for cell transplantation or disease modelling using directed differentiation of embryonic stem cells or induced pluripotent stem cells, or in vivo via direct reprogramming of resident adult cardiac fibroblast or ectopic stimulation of resident cardiac stem or progenitor cells. A major bottleneck with all of these approaches is the low efficiency of cardiomyocyte differentiation alongside their relative functional immaturity. Chemical genetics, and the application of phenotypic screening with small molecule libraries, represent a means to enhance understanding of the molecular pathways controlling cardiovascular cell differentiation and, moreover, offer the potential for discovery of new drugs to invoke heart repair and regeneration. Here, we review the potential of chemical genetics in cardiac stem cell therapy, highlighting not only the major contributions to the field so far, but also the future challenges. LINKED ARTICLES This article is part of a themed section on Regenerative Medicine and Pharmacology: A Look to the Future. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2013.169.issue-2 PMID:22385148

Queer in STEM: Workplace Experiences Reported in a National Survey of LGBTQA Individuals in Science, Technology, Engineering, and Mathematics Careers.

PubMed

Yoder, Jeremy B; Mattheis, Allison

2016-01-01

A survey of individuals working in science, technology, engineering, and mathematics (STEM) fields who identify as lesbian, gay, bisexual, trans*, queer, or asexual (LGTBQA) was administered online in 2013. Participants completed a 58-item questionnaire to report their professional areas of expertise, levels of education, geographic location, and gender and sexual identities and rated their work and social communities as welcoming or hostile to queer identities. An analysis of 1,427 responses to this survey provided the first broad portrait of this population, and it revealed trends related to workplace practices that can inform efforts to improve queer inclusivity in STEM workplaces.

Understanding Middle School Students' Perceptions of Physics Using Girl-Friendly and Integrated STEM Strategies: A Gender Study

NASA Astrophysics Data System (ADS)

Dare, Emily Anna

According to the American Physical Society, women accounted for only 20% of bachelor's degrees in the fields of physics and engineering in 2010. This low percentage is likely related to young girls' K-12 education experiences, particularly their experiences prior to high school, during which time young women's perceptions of Science, Technology, Engineering, and Math (STEM) and STEM careers are formed (Catsambis, 1995; Maltese & Tai, 2011; National Research Council, 2012; Sadler, Sonnert, Hazari, & Tai, 2012; Tai, Liu, Maltese, & Fan, 2006; Scantlebury, 2014; Sikora & Pokropek, 2012). There are no significant gender differences in academic achievement in middle school, yet young women have less positive attitudes towards careers in science than their male peers (Catsambis, 1995; Scantlebury, 2014). This suggests that the low female representation in certain STEM fields is a result of not their abilities, but their perceptions; for fields like physics where negative perceptions persist (Haussler & Hoffman, 2002; Labudde, Herzog, Neuenschander, Violi, & Gerber, 2000), it is clear that middle school is a critical time to intervene. This study examines the perceptions of 6th grade middle school students regarding physics and physics-related careers. A theoretical framework based on the literature of girl-friendly and integrated STEM strategies (Baker & Leary, 1995; Halpern et al., 2007; Haussler & Hoffman, 2000, 2002; Labudde et al., 2000; Moore et al., 2014b; Newbill & Cennamo, 2008; Rosser, 2000; Yanowitz, 2004) guided this work to understand how these instructional strategies may influence student's perceptions of physics for both girls and boys. The overarching goal of this work was to understand similarities and differences between girls' and boys' perceptions about physics and physics-related careers. This convergent parallel mixed-methods study uses a series of student surveys and focus group interviews to identify and understand these similarities and differences. Classroom observations also helped to identify what instructional strategies teachers used that influence student perceptions. Findings from this study indicate very few differences between the perceptions of physics and physics-related careers for 6th grade girls and boys. However, the differences that exist, though subtle, may indicate how K-12 science instruction could more positively influence girls' perceptions. For instance, while girls are just as interested in science class as their male counterparts, they are more motivated when a social context is included; this has implications for how they view physics-related careers. The findings of this study shed light on not only why fewer females pursue careers in physics, but also how K-12 science reform efforts might help to increase these numbers.

Examining the Impact of Afterschool STEM Programs

ERIC Educational Resources Information Center

Krishnamurthi, Anita; Ballard, Melissa; Noam, Gil G.

2014-01-01

Afterschool programs that provide strong science, technology, engineering and math (STEM) learning experiences are making an impact on participating youth not only become excited and engaged in these fields but develop STEM skills and proficiencies, come to value these fields and their contributions to society, and--significantly--begin to see…

An Innovative Postsecondary Education Program for Students with Disabilities in STEM (Practice Brief)

ERIC Educational Resources Information Center

Dunn, Caroline; Shannon, David; McCullough, Brittany; Jenda, Overtoun; Qazi, Mohammed

2018-01-01

Careers in the science, technology, engineering, and mathematics (STEM) fields have many benefits, including decent salaries, a strong employment outlook, and high job satisfaction. Unfortunately, workers with disabilities are underrepresented in the STEM fields. This practice brief describes a program designed to support college students with…

Toward Inclusive STEM Classrooms: What Personal Role Do Faculty Play?

PubMed Central

Killpack, Tess L.; Melón, Laverne C.

2016-01-01

Private and public policies are increasingly aimed at supporting efforts to broaden participation of a diverse body of students in higher education. Unfortunately, this increase in student diversity does not always occur alongside changes in institutional culture. Unexamined biases in institutional culture can prevent diverse students from thriving and persisting in science, technology, engineering, and mathematics (STEM) fields. Given the daily personal interactions that faculty have with students, we suggest that individual educators have the opportunity, and responsibility, to improve the retention and persistence of diverse students. However, in our experience, faculty professional development programs often limit discussions of diversity to “comfortable” topics (such as learning styles) and miss opportunities to explore deeper issues related to faculty privilege, implicit bias, and cues for stereotype threat that we all bring to the classroom. In this essay, we present a set of social science concepts that we can extend to our STEM courses to inform our efforts at inclusive excellence. We have recommended strategies for meaningful reflection and professional development with respect to diversity and inclusion, and aim to empower faculty to be change agents in their classrooms as a means to broadening participation in STEM fields. PMID:27496362

Resistance in mango against infection by Ceratocystis fimbriata.

PubMed

Araujo, Leonardo; Bispo, Wilka Messner Silva; Cacique, Isaías Severino; Moreira, Wiler Ribas; Rodrigues, Fabrício Ávila

2014-08-01

This study was designed to characterize and describe host cell responses of stem tissue to mango wilt disease caused by the fungus Ceratocystis fimbriata in Brazil. Disease progress was followed, through time, in inoculated stems for two cultivars, 'Ubá' (field resistant) and 'Haden' (field susceptible). Stem sections from inoculated areas were examined using fluorescence light microscopy and transmission and scanning electron microscopy, coupled with energy-dispersive X-ray microanalysis. Tissues from Ubá colonized by C. fimbriata had stronger autofluorescence than those from Haden. The X-ray microanalysis revealed that the tissues of Ubá had higher levels of insoluble sulfur and calcium than those of Haden. Scanning electron microscopy revealed that fungal hyphae, chlamydospores (aleurioconidia), and perithecia-like structures of C. fimbriata were more abundant in Haden relative to Ubá. At the ultrastructural level, pathogen hyphae had grown into the degraded walls of parenchyma, fiber cells, and xylem vessels in the tissue of Haden. However, in Ubá, plant cell walls were rarely degraded and hyphae were often surrounded by dense, amorphous granular materials and hyphae appeared to have died. Taken together, the results of this study characterize the susceptible and resistant basal cell responses of mango stem tissue to infection by C. fimbriata.

A Case Study of an Academia-Industry Partnership to Meet the Education and Training Needs in a Science, Technology, Engineering & Mathematics (STEM) Field

ERIC Educational Resources Information Center

Richardson, Joseph Carl

2013-01-01

The purpose of this case study is to provide a description of the characteristics of an academia-industry partnership that works together with industry to meet the education and training needs in a Science, Technology, Engineering, and Mathematics (STEM) field. After the launch of Sputnik in 1957, U.S. pursued efforts to compete in STEM fields on…

Kidney regeneration by xeno-embryonic nephrogenesis.

PubMed

Yokoo, Takashi; Fukui, Akira; Matsumoto, Kei; Kawamura, Tetsuya

2008-03-01

Establishment of a functional whole kidney de novo has not received much attention because of the formidable challenges and the slow pace of advances in this field of research. This situation has changed recently with publication of data revealing the catastrophic nature of Medicaid costs for dialysis-related diseases. An innovative approach is needed in our search for therapies for kidney diseases and to provide a substitute for dialysis as soon as possible. Regenerative medicine offers great hope for realizing this goal. We established a system by which human mesenchymal stem cells can differentiate into a functional renal unit using a program of nephrogenesis in a developing xeno-embryo. In this article, recent research in the field of developing whole kidneys is reviewed, and possible therapeutic applications for kidney diseases are proposed in combination with our knowledge of the emerging field of kidney stem cell biology.

Farming system and wheat cultivar affect infestation of, and parasitism on, Cephus cinctus in the Northern Great Plains.

PubMed

Adhikari, Subodh; Seipel, Tim; Menalled, Fabian D; Weaver, David K

2018-03-26

Cephus cinctus infestation causes $350 million in annual losses in the Northern Great Plains. We compared infestation and parasitism of C. cinctus in spring (including Kamut; Triticum turgidum ssp. turanicum) and winter wheat cultivars grown in organic and conventional fields in Montana, USA. In the greenhouse, we compared C. cinctus preference and survival in Kamut, Gunnison, and Reeder spring wheat cultivars. Stems cut by C. cinctus varied by farming system and the seasonality of the wheat crop. No stems of Kamut in organic fields were cut by C. cinctus, but 1.5% [±0.35% standard error (SE)] of stems in conventional spring wheat, 5% (±0.70% SE) of stems in organic winter wheat, and 20% (±0.93% SE) of stems in conventional winter wheat fields were cut by C. cinctus. More larvae of C. cinctus were parasitized in organic (27 ± 0.03% SE) compared with conventional (5 ± 0.01% SE) winter wheat fields. Cephus cinctus oviposition, survival, and the number of stems cut were lowest in Kamut compared with Gunnison and Reeder. Cephus cinctus infestation was more common in winter wheat than in spring wheat. Organic fields with fewer cut stems also supported more parasitoids. Kamut is a genetic resource for developing C. cinctus-resistant cultivars. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.

[Related issues in clinical translational application of adipose-derived stem cells].

PubMed

Liu, Hongwei; Cheng, Biao; Fu, Xiaobing

2012-10-01

To introduce the related issues in the clinical translational application of adipose-derived stem cells (ASCs). The latest papers were extensively reviewed, concerning the issues of ASCs production, management, transportation, use, and safety during clinical application. ASCs, as a new member of adult stem cells family, bring to wide application prospect in the field of regenerative medicine. Over 40 clinical trials using ASCs conducted in 15 countries have been registered on the website (http://www.clinicaltrials.gov) of the National Institutes of Health (NIH), suggesting that ASCs represents a promising approach to future cell-based therapies. In the clinical translational application, the related issues included the quality control standard that management and production should follow, the prevention measures of pathogenic microorganism pollution, the requirements of enzymes and related reagent in separation process, possible effect of donor site, age, and sex in sampling, low temperature storage, product transportation, and safety. ASCs have the advantage of clinical translational application, much attention should be paid to these issues in clinical application to accelerate the clinical translation process.

The Need for an Effective Collaboration across Science, Technology, Engineering & Mathematics (STEM) Fields for a Meaningful Technological Development in Nigeria

ERIC Educational Resources Information Center

Haruna, Umar Ibrahim

2015-01-01

Collaboration plays a major role in interdisciplinary activities among Science, Technology, Engineering & Mathematics (STEM) disciplines or fields. It also affects the relationships among cluster members on the management team. Although effective collaboration does not guarantee success among STEM disciplines, its absence usually assures…

Insights into Implementing Research Collaborations between Research-Intensive Universities and Minority-Serving Institutions

ERIC Educational Resources Information Center

Thao, Mao; Lawrenz, Frances; Brakke, Mary; Sherman, Jamie; Matute, Martin

2016-01-01

With the high demand to build the science, technology, engineering, and mathematics (STEM) workforce and the disparity of underrepresented minorities in STEM fields, there have been increased educational efforts to diversify STEM fields. This article describes what works in research collaborations between research-intensive universities (RIUs) and…

STEM Specialty Programs: A Pathway for Under-Represented Students into STEM Fields

ERIC Educational Resources Information Center

Alvarez, Charles Alex; Edwards, Douglas; Harris, Bonnie

2010-01-01

Addressing the under-representation of women, minorities, and persons with disabilities in science, technology, engineering, and mathematics (STEM) fields has been an initiative of the U.S. Congress for the past 30 years, but the challenge still remains unresolved. The National Science Foundation (NSF) and the Congressional Committee on Equal…

Gender Differences in Expressed Interests in Engineering-Related Fields ACT 30-Year Data Analysis Identified Trends and Suggested Avenues to Reverse Trends

ERIC Educational Resources Information Center

Iskander, E. Tiffany; Gore, Paul A., Jr.; Furse, Cynthia; Bergerson, Amy

2013-01-01

Historically, women have been underrepresented in the Science, Technology, Engineering, and Math (STEM) fields both as college majors and in the professional community. This disturbing trend, observed in many countries, is more serious and evident in American universities and is reflected in the U.S. workforce statistics. In this article, we…

The influence of stem initiative programs for middle and high school students on female STEM college majors

NASA Astrophysics Data System (ADS)

McCaslin, Stephanie D.

The areas of Science, Technology, Engineering, and Mathematics have long been overrepresented by men. In the workforce, more men work in these fields than women, and in school, more male students select majors in Science, Technology, Engineering, and Mathematics (STEM) than female students. Research has indicated that female students represent less than a third of college students selecting STEM majors. Several recommendations have been made by prominent educational organizations, such as the American Association of University Women (AAUW), including promoting these subjects to female students through STEM initiatives that are innovative and expose female students to careers in these areas. This qualitative research study sought to analyze the effectiveness of these initiatives by determining what factors are considered when a female student selects a STEM field of study at the college level and to examine how these students perceived the effectiveness of the STEM initiatives in which they participated. A series of interviews were conducted with female college students with declared majors in STEM fields who had participated in STEM initiatives in the state of Maryland. After analysis of the data collected, it was determined that STEM initiatives are not necessarily effective in increasing the number of women who enroll in STEM programs at the college level, however, they are effective in encouraging female students who are already interested in STEM. Female students who participated in these STEM initiatives more frequently were more likely to have a better understanding of STEM options, and were also more likely to complete STEM college degrees in less time than those who did not participate frequently in STEM initiatives.

Unstable identity compatibility: how gender rejection sensitivity undermines the success of women in science, technology, engineering, and mathematics fields.

PubMed

Ahlqvist, Sheana; London, Bonita; Rosenthal, Lisa

2013-09-01

Although the perceived compatibility between one's gender and science, technology, engineering, and mathematics (STEM) identities (gender-STEM compatibility) has been linked to women's success in STEM fields, no work to date has examined how the stability of identity over time contributes to subjective and objective STEM success. In the present study, 146 undergraduate female STEM majors rated their gender-STEM compatibility weekly during their freshman spring semester. STEM women higher in gender rejection sensitivity, or gender RS, a social-cognitive measure assessing the tendency to perceive social-identity threat, experienced larger fluctuations in gender-STEM compatibility across their second semester of college. Fluctuations in compatibility predicted impaired outcomes the following school year, including lower STEM engagement and lower academic performance in STEM (but not non-STEM) classes, and significantly mediated the relationship between gender RS and STEM engagement and achievement in the 2nd year of college. The week-to-week changes in gender-STEM compatibility occurred in response to negative academic (but not social) experiences.

Stem cell bioprinting for applications in regenerative medicine.

PubMed

Tricomi, Brad J; Dias, Andrew D; Corr, David T

2016-11-01

Many regenerative medicine applications seek to harness the biologic power of stem cells in architecturally complex scaffolds or microenvironments. Traditional tissue engineering methods cannot create such intricate structures, nor can they precisely control cellular position or spatial distribution. These limitations have spurred advances in the field of bioprinting, aimed to satisfy these structural and compositional demands. Bioprinting can be defined as the programmed deposition of cells or other biologics, often with accompanying biomaterials. In this concise review, we focus on recent advances in stem cell bioprinting, including performance, utility, and applications in regenerative medicine. More specifically, this review explores the capability of bioprinting to direct stem cell fate, engineer tissue(s), and create functional vascular networks. Furthermore, the unique challenges and concerns related to bioprinting living stem cells, such as viability and maintaining multi- or pluripotency, are discussed. The regenerative capacity of stem cells, when combined with the structural/compositional control afforded by bioprinting, provides a unique and powerful tool to address the complex demands of tissue engineering and regenerative medicine applications. © 2016 New York Academy of Sciences.

The Science and Ethics of Induced Pluripotency: What Will Become of Embryonic Stem Cells?

PubMed Central

Zacharias, David G.; Nelson, Timothy J.; Mueller, Paul S.; Hook, C. Christopher

2011-01-01

For over a decade, the field of stem cell research has advanced tremendously and gained new attention in light of novel insights and emerging developments for regenerative medicine. Invariably, multiple considerations come into play, and clinicians and researchers must weigh the benefits of certain stem cell platforms against the costs they incur. Notably, human embryonic stem (hES) cell research has been a source of continued debate, leading to differing policies and regulations worldwide. This article briefly reviews current stem cell platforms, looking specifically at the two existing pluripotent lines available for potential therapeutic applications: hES cells and induced pluripotent stem (iPS) cells. We submit iPS technology as a viable and possibly superior alternative for future medical and research endeavors as it obviates many ethical and resource-related concerns posed by hES cells while prospectively matching their potential for scientific use. However, while the clinical realities of iPS cells appear promising, we must recognize the current limitations of this technology, avoid hype, and articulate ethically acceptable medical and scientific goals. PMID:21719620

Curvature in Arabidopsis inflorescence stems is limited to the region of amyloplast displacement

NASA Technical Reports Server (NTRS)

Weise, S. E.; Kuznetsov, O. A.; Hasenstein, K. H.; Kiss, J. Z.

2000-01-01

Gravitropic sensing in stems and stem-like organs is hypothesized to occur in the endodermis. However, since the endodermis runs the entire length of the stem, the precise site of gravisensing has been difficult to define. In this investigation of gravisensitivity in inflorescence stems of Arabidopsis, we positioned stems in a high gradient magnetic field (HGMF) on a rotating clinostat. Approximately 40% of the young, wild-type (WT) inflorescences, for all positions tested, curved toward the HGMF in the vicinity of the stem exposed to the field. In contrast, when the wedge was placed in the basal region of older inflorescence stems, no curvature was observed. As a control, the HGMF was applied to a starchless mutant, and 5% of the stems curved toward the field. Microscopy of the endodermis in the WT showed amyloplast displacement in the vicinity of the HGMF. Additional structural studies demonstrated that the basal region of WT stems experienced amyloplast displacement and, therefore, suggest this region is capable of gravity perception. However, increased lignification likely prevented curvature in the basal region. The lack of apical curvature after basal amyloplast displacement indicates that gravity perception in the base is not transmitted to the apex. Thus, these results provide evidence that the signal (and thus, response) resulting from perception in Arabidopsis inflorescence stems is spatially restricted.

The creation and validation of an instrument to measure school STEM Culture

NASA Astrophysics Data System (ADS)

White, Christopher

Although current research exists on school culture, there is a gap in the literature on specialized aspects of culture such as STEM Culture defined as the beliefs, values, practices, resources, and challenges in STEM fields (Science, Technology, Engineering and Mathematics) within a school. The objective of this study was to create a valid and reliable instrument, the STEM Culture Assessment Tool (STEM-CAT), that measures this cultural aspect based on a survey of stakeholder groups within the school community and use empirical data to support the use of this instrument to measure STEM Culture. Items were created and face validity was determined through a focus group and expert review before a pilot study was conducted to determine reliability of the items. Once items were determined reliable, the survey was given to eight high schools and results were correlated to the percentage of seniors who self-reported whether they intend to pursue STEM fields upon graduation. The results of this study indicate further need for research to determine how the STEM-CAT correlates to STEM culture due to some inconsistencies with the dependent variable in this study. Future research could be done correlating the results of the STEM-CAT with participation in Advanced Placement science and mathematics, SAT/ACT scores in science and mathematics or the number of students who actually pursue STEM fields rather than a prediction halfway through the 12th grade.

(Re-)programming of subtype specific cardiomyocytes.

PubMed

Hausburg, Frauke; Jung, Julia Jeannine; Hoch, Matti; Wolfien, Markus; Yavari, Arash; Rimmbach, Christian; David, Robert

2017-10-01

Adult cardiomyocytes (CMs) possess a highly restricted intrinsic regenerative potential - a major barrier to the effective treatment of a range of chronic degenerative cardiac disorders characterized by cellular loss and/or irreversible dysfunction and which underlies the majority of deaths in developed countries. Both stem cell programming and direct cell reprogramming hold promise as novel, potentially curative approaches to address this therapeutic challenge. The advent of induced pluripotent stem cells (iPSCs) has introduced a second pluripotent stem cell source besides embryonic stem cells (ESCs), enabling even autologous cardiomyocyte production. In addition, the recent achievement of directly reprogramming somatic cells into cardiomyocytes is likely to become of great importance. In either case, different clinical scenarios will require the generation of highly pure, specific cardiac cellular-subtypes. In this review, we discuss these themes as related to the cardiovascular stem cell and programming field, including a focus on the emergent topic of pacemaker cell generation for the development of biological pacemakers and in vitro drug testing. Copyright © 2017 Elsevier B.V. All rights reserved.

Nanotechnology in the regulation of stem cell behavior

NASA Astrophysics Data System (ADS)

Wu, King-Chuen; Tseng, Ching-Li; Wu, Chi-Chang; Kao, Feng-Chen; Tu, Yuan-Kun; So, Edmund C.; Wang, Yang-Kao

2013-10-01

Stem cells are known for their potential to repair damaged tissues. The adhesion, growth and differentiation of stem cells are likely controlled by the surrounding microenvironment which contains both chemical and physical cues. Physical cues in the microenvironment, for example, nanotopography, were shown to play important roles in stem cell fate decisions. Thus, controlling stem cell behavior by nanoscale topography has become an important issue in stem cell biology. Nanotechnology has emerged as a new exciting field and research from this field has greatly advanced. Nanotechnology allows the manipulation of sophisticated surfaces/scaffolds which can mimic the cellular environment for regulating cellular behaviors. Thus, we summarize recent studies on nanotechnology with applications to stem cell biology, including the regulation of stem cell adhesion, growth, differentiation, tracking and imaging. Understanding the interactions of nanomaterials with stem cells may provide the knowledge to apply to cell-scaffold combinations in tissue engineering and regenerative medicine.

Preparing Women for Leadership Roles in STEM: The HERS Institutes

NASA Astrophysics Data System (ADS)

Straub, K. H.

2016-12-01

While women's representation in higher education has increased at the undergraduate and graduate levels, female voices remain in the minority in upper administrative positions, particularly in STEM fields. The HERS Institutes prepare women faculty and administrators for leadership roles through either residential (2 week, summer) or mixed on-site and online (academic year) programs. Topics addressed through the HERS curriculum include managing and leading change, the financial environment of higher education, diversity and inclusion, career mapping, fundraising, the legal landscape, institutional budgeting, negotiation, search essentials, conflict management, and much more. Female experts in each field lead interactive sessions and discussions, and are available for one-on-one conversations during breaks and meals. Through a grant from the Henry Luce Foundation, HERS has created opportunities for women in STEM fields to receive scholarships to the Institutes through the Claire Booth Luce (CBL) Program. In addition, the Institutes now support a larger cohort of STEM participants through targeted programming and networking events. Of the 64 participants in the 2016 Bryn Mawr Institute, 21 women were from STEM fields. These women participated in additional programs and expert-led discussions including "The Future of Research in Higher Education," "Supporting Careers of Women in STEM," and "Inclusive Excellence in STEM." An additional component of the CBL Program is the creation of a HERS STEM Community to enhance networking, mentoring, information sharing, and opportunities for gathering at professional conferences among HERS alumnae.

Gendered transitions to adulthood by college field of study in the United States.

PubMed

Han, Siqi; Tumin, Dmitry; Qian, Zhenchao

2016-01-01

Field of study may influence the timing of transitions to the labor market, marriage, and parenthood among college graduates. Research to date has yet to study how field of study is associated with the interweaving of these transitions in the USA. The current study examines gendered influences of college field of study on transitions to a series of adult roles, including full-time work, marriage, and parenthood. We use Cox proportional hazards models and multinomial logistic regression to examine gendered associations between field of study and the three transitions among college graduates of the NLSY97 (National Longitudinal Survey of Youth) cohort. Men majoring in STEM achieve early transitions to full-time work, marriage, and parenthood; women majoring in STEM show no significant advantage in finding full-time work and delayed marriage and childbearing; women in business have earlier transitions to full-time work and marriage than women in other fields, demonstrating an advantage similar to that of men in STEM. The contrast between men and women in STEM shows that transition to adulthood remains gendered; the contrast between women in STEM and women in business illustrates that a prestigious career may not necessarily delay family formation.

Learning from our global competitors: A comparative analysis of science, technology, engineering and mathematics (STEM) education pipelines in the United States, Mainland China and Taiwan

NASA Astrophysics Data System (ADS)

Chow, Christina M.

Maintaining a competitive edge within the 21st century is dependent on the cultivation of human capital, producing qualified and innovative employees capable of competing within the new global marketplace. Technological advancements in communications technology as well as large scale, infrastructure development has led to a leveled playing field where students in the U.S. will ultimately be competing for jobs with not only local, but also international, peers. Thus, the ability to understand and learn from our global competitors, starting with the examination of innovative education systems and best practice strategies, is tantamount to the economic development, and ultimate survival, of the U.S. as a whole. The purpose of this study was to investigate the current state of science, technology, engineering and mathematics (STEM) education and workforce pipelines in the U.S., China, and Taiwan. Two broad research questions examined STEM workforce production in terms of a) structural differences in primary and secondary school systems, including analysis of minimum high school graduation requirements and assessments as well as b) organizational differences in tertiary education and trends in STEM undergraduate and graduate degrees awarded in each region of interest. While each of the systems studied had their relative strengths and weaknesses, each of the Asian economies studied had valuable insights that can be categorized broadly in terms of STEM capacity, STEM interest and a greater understanding of global prospects that led to heightened STEM awareness. In China and Taiwan, STEM capacity was built via both traditional and vocational school systems. Focused and structured curriculum during the primary and early secondary school years built solid mathematics and science skills that translated into higher performance on international assessments and competitions. Differentiated secondary school options, including vocational high school and technical colleges and programs beginning shortly after junior high produced a greater number of alternatives for producing STEM capable students. A heightened interest in the STEM fields was built upon standardized academic core curriculum that ultimately yielded a greater percentage of qualified and interested Asian students pursuing bachelor's and advanced STEM degrees both in their native country and abroad. Rewards and incentives built into school systems, expansion of tertiary degree-granting programs, as well as the development of multiple university entrance pathways has served to heighten interest and perception of STEM careers as well as recruit top students into STEM fields. Further, foreign language classes, starting from either the first or third year of primary school, coupled with information technology and other experimental science and research themed classes, resulted in students who were more aware of global market demands. Analysis of longitudinal data shows that over a nine-year period, this combination of increased STEM capacity, interest and awareness resulted in a far greater percentage of 9th graders who eventually became STEM certificate, bachelor's, and advanced degree holders capable of competing in the global marketplace.

Undergraduate women in STEM: Does participation in STEM extracurricular programs enhance success among students?

NASA Astrophysics Data System (ADS)

Price, Kasey Marie

Women have been underrepresented in the STEM fields since the 1650's to today (Hunter, 2005). This study examined the extracurricular participation of undergraduate women, in Fall 2009, using both quantitative and qualitative methods, who were majoring in at least one (1) of the 49 STEM majors at Southeastern State University participated in STEM extracurricular programs and if any specific program contributed to success more than other programs. A second question was whether participation in an extracurricular program(s) influenced their success. Women who were older, had been enrolled more semesters, had more credit hours, and had families with higher incomes were more likely to be involved in STEM only or STEM and Non-STEM extracurricular activities. Additionally, students who completed a high level of high school math, had a higher high school GPA, had received a regular high school diploma, and who had mothers with a higher level of education were also more likely to be involved in STEM only or STEM and Non-STEM extracurricular activities. Students who had been enrolled in college seven (7) or more semesters, who had selected their current major within their first year of college, were more likely to be involved in STEM extracurricular activities. Students believe that their STEM extracurricular involvement helps them to be successful because it provided them with student relationships, opportunity for the future, advising relationships, mentorship, and exploration of the campus and larger community. This study may be useful for student affairs professionals and academics who take an active role in serving as advisors, mentors, and providers of STEM-related opportunities.

Stem cells in sepsis and acute lung injury.

PubMed

Cribbs, Sushma K; Matthay, Michael A; Martin, Greg S

2010-12-01

Sepsis and acute lung injury continue to be major causes of morbidity and mortality worldwide despite advances in our understanding of pathophysiology and the discovery of new management strategies. Recent investigations show that stem cells may be beneficial as prognostic biomarkers and novel therapeutic strategies in these syndromes. This article reviews the potential use of endogenous adult tissue-derived stem cells in sepsis and acute lung injury as prognostic markers and also as exogenous cell-based therapy. A directed systematic search of the medical literature using PubMed and OVID, with particular emphasis on the time period after 2002, was done to evaluate topics related to 1) the epidemiology and pathophysiology of sepsis and acute lung injury; and 2) the definition, characterization, and potential use of stem cells in these diseases. DATA SYNTHESIS AND FINDINGS: When available, preferential consideration was given to prospective nonrandomized clinical and preclinical studies. Stem cells have shown significant promise in the field of critical care both for 1) prognostic value and 2) treatment strategies. Although several recent studies have identified the potential benefit of stem cells in sepsis and acute lung injury, further investigations are needed to more completely understand stem cells and their potential prognostic and therapeutic value.

Aging and reprogramming: a two-way street

PubMed Central

Mahmoudi, Salah; Brunet, Anne

2012-01-01

Aging is accompanied by the functional decline of cells, tissues, and organs, as well as a striking increase in a wide range of diseases. The reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) opens new avenues for the aging field and has important applications for therapeutic treatments of age-related diseases. Here we review emerging studies on how aging and age-related pathways influence iPSC generation and property. We discuss the exciting possibility that reverting to a pluripotent stem cell stage erases several deficits associated with aging and will provide new strategies for rejuvenation. Finally, we argue that reprogramming provides a unique opportunity to model aging and perhaps exceptional longevity. PMID:23146768

Factors motivating Cambodian American students to go to college and to study STEM fields

NASA Astrophysics Data System (ADS)

Sann, Visna

Cambodian Americans graduate from college at a lower rate than most Asian American groups. This qualitative study involved interviewing five current Cambodian American college students. This study examined how participants' high school experiences contributed to their decisions to go to college and to study STEM fields, how parental influences guided participants to college, and how college experiences influenced their decisions to stay and succeed in STEM fields. Findings from this study suggest: having supportive teachers in high school may have been important in motivating participants to go to college and to study STEM Fields, Cambodian parents tell stories of their lives in Cambodia to motivate their children to go to college, and Cambodian club on campus was a socially and academically supportive place.

High School Math and Science Preparation and Postsecondary STEM Participation for Students with an Autism Spectrum Disorder

ERIC Educational Resources Information Center

Wei, Xin; Yu, Jennifer; Shattuck, Paul; Blackorby, Jose

2017-01-01

Previous studies suggest that individuals with an Autism Spectrum Disorder (ASD) are more likely than other disability groups and the general population to gravitate toward science, technology, engineering, and mathematics (STEM) fields. However, the field knows little about which factors influenced the STEM pipeline between high school and…

High School Math and Science Preparation and Postsecondary STEM Participation for Students with an Autism Spectrum Disorder

ERIC Educational Resources Information Center

Wei, Xin; Yu, Jennifer W.; Shattuck, Paul; Blackorby, Jose

2017-01-01

Previous studies suggest that individuals with an autism spectrum disorder (ASD) are more likely than other disability groups and the general population to gravitate toward science, technology, engineering, and mathematics (STEM) fields. However, the field knows little about which factors influence the STEM pipeline between high school and…

Beyond Smash and Crash: Gender-Friendly Tech Ed

ERIC Educational Resources Information Center

McCarthy, Ray

2009-01-01

In order to increase participation in science, technology, engineering, and math (STEM) fields and careers, one of the problems that needs to be addressed is gender equity of study and careers in STEM fields. In general, women represent less than 30% of all STEM students in college. Furthermore, less than one third of professional engineers and…

Factors that promote success in women enrolled in STEM disciplines in rural North Carolina community colleges

NASA Astrophysics Data System (ADS)

Kincaid, Shannon D.

Women have historically been underrepresented in the fields of science, technology, engineering, and math (STEM fields). The underrepresentation of women in STEM may be attributable to a variety of factors. These may include different choices men and women typically make in response to incentives in STEM education. For example, STEM career paths may be less accommodating to people who are less resilient. Another factor may be that there are relatively few female STEM role models. Perhaps strong gender stereotypes discourage women from pursuing STEM education and STEM jobs. The factors that contribute to success and the barriers that impeded success must be identified before any steps can be taken to improve the educational outcomes for women in STEM disciplines. Consequently, relatively little is known about the role of resilience in academically successful adult women in rural community colleges enrolled in STEM disciplines and the mechanisms that underlie the performance deficits that occur as a result of stereotype threat effect. This mixed method study addressed those knowledge gaps by determining: (1) if high resilience is positively correlated to high grade point average for women enrolled in STEM disciplines in rural community colleges in North Carolina, and (2) if stereotype threat effect is a risk factor for these women. Quantitative data were collected by using "The Resilience Scale" (Wagnild & Young, 1987) and through examination of grade point average of students from Datatel data management software. Qualitative data were collected through semi-structured focus group interviews. Findings from this study indicate high resilience is positively correlated to high grade point average for women enrolled in STEM disciplines in rural community colleges in North Carolina, and stereotype threat effect was a risk factor for low-scoring women (i.e. those women who reported resilience scores less than 121 and grade point averages lower than 2.70) and was not a risk factor for high-scoring women (i.e. those women who reported resilience scores of 147 or higher and grade point averages of 2.70 or higher). Overall, qualitative data analysis revealed both high-scoring and low-scoring women in STEM disciplines were affected by stereotype threat effect. However, low-scoring women were negatively impacted by stereotype threat and high-scoring women were able to use pressures associated with stereotype threat as motivation for success. Based on results from this study four principal factors were found that influence the success of women in STEM disciplines. These factors include elimination of stereotype threat, enhancement of resilience of female students, expansion of female gender representation on community college campuses, and development of positive instructor-student and advisor-student relationships. While this study does not, and cannot, explain why gender differences in STEM exist, it does provide data and insight that will enable more informed policymaking for community college administrators in order to increase success of women in STEM disciplines. The findings provide definitive evidence of a need to encourage and support women in STEM education with a goal of gender parity.

Korean Students' Attitudes toward STEM Project-Based Learning and Major Selection

ERIC Educational Resources Information Center

Han, Sunyoung

2017-01-01

The trend of avoiding science, technology, engineering, and mathematics (STEM) majors has persisted resulting in a lack of professionals in STEM fields. Further, the current STEM education system in Korea does not meet domestic demands for STEM labor. To discover an educational approach encouraging students to choose STEM majors at the…

T-cell and natural killer cell therapies for hematologic malignancies after hematopoietic stem cell transplantation: enhancing the graft-versus-leukemia effect

PubMed Central

Cruz, C. Russell; Bollard, Catherine M.

2015-01-01

Hematopoietic stem cell transplantation has revolutionized the treatment of hematologic malignancies, but infection, graft-versus-host disease and relapse are still important problems. Calcineurin inhibitors, T-cell depletion strategies, and immunomodulators have helped to prevent graft-versus-host disease, but have a negative impact on the graft-versus-leukemia effect. T cells and natural killer cells are both thought to be important in the graft-versus-leukemia effect, and both cell types are amenable to ex vivo manipulation and clinical manufacture, making them versatile immunotherapeutics. We provide an overview of these immunotherapeutic strategies following hematopoietic stem cell transplantation, with discussions centered on natural killer and T-cell biology. We discuss the contributions of each cell type to graft-versus-leukemia effects, as well as the current research directions in the field as related to adoptive cell therapy after hematopoietic stem cell transplantation. PMID:26034113

An examination of undergraduate engineering students' stereotype of scientists and their career intentions

NASA Astrophysics Data System (ADS)

Stara, Michelle M.

The US Government Accountability Office (GAO) (2013) has acknowledged that additional graduates are needed in engineering and related STEM fields. However, the GAO has also noted that it is difficult to determine if the additional graduates will align with employer demand at the time of entry into the workforce. This research study attempts to examine undergraduate engineering students' perceptions of scientists and if they were related to students' intentions to pursue science by examining the constructs of Stereotypes of Scientists (SOS) and Career Intentions in Science (CIS). While results of data analysis were not significant, patterns were seen that provided valuable information with regard to the variability of undergraduate engineering students and the complexity of what goes into stereotype formation and career choice. As a practitioner, there were pertinent applications that could be implemented from the results of this and related studies. From the perspective of practitioners, the findings may be used to target recruitment, retention, and specific teaching strategies to increase enrollment and graduate numbers in the lesser known engineering and STEM fields.

An empirical method that separates irreversible stem radial growth from bark water content changes in trees: theory and case studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mencuccini, Maurizio; Salmon, Yann; Mitchell, Patrick

Substantial uncertainty surrounds our knowledge of tree stem growth, with some of the most basic questions, such as when stem radial growth occurs through the daily cycle, still unanswered. Here, we employed high-resolution point dendrometers, sap flow sensors, and developed theory and statistical approaches, to devise a novel method separating irreversible radial growth from elastic tension-driven and elastic osmotically driven changes in bark water content. We tested this method using data from five case study species. Experimental manipulations, namely a field irrigation experiment on Scots pine and a stem girdling experiment on red forest gum trees, were used to validatemore » the theory. Time courses of stem radial growth following irrigation and stem girdling were consistent with a-priori predictions. Patterns of stem radial growth varied across case studies, with growth occurring during the day and/or night, consistent with the available literature. Importantly, our approach provides a valuable alternative to existing methods, as it can be approximated by a simple empirical interpolation routine that derives irreversible radial growth using standard regression techniques. In conclusion, our novel method provides an improved understanding of the relative source–sink carbon dynamics of tree stems at a sub-daily time scale.« less

An empirical method that separates irreversible stem radial growth from bark water content changes in trees: theory and case studies.

PubMed

Mencuccini, Maurizio; Salmon, Yann; Mitchell, Patrick; Hölttä, Teemu; Choat, Brendan; Meir, Patrick; O'Grady, Anthony; Tissue, David; Zweifel, Roman; Sevanto, Sanna; Pfautsch, Sebastian

2017-02-01

Substantial uncertainty surrounds our knowledge of tree stem growth, with some of the most basic questions, such as when stem radial growth occurs through the daily cycle, still unanswered. We employed high-resolution point dendrometers, sap flow sensors, and developed theory and statistical approaches, to devise a novel method separating irreversible radial growth from elastic tension-driven and elastic osmotically driven changes in bark water content. We tested this method using data from five case study species. Experimental manipulations, namely a field irrigation experiment on Scots pine and a stem girdling experiment on red forest gum trees, were used to validate the theory. Time courses of stem radial growth following irrigation and stem girdling were consistent with a-priori predictions. Patterns of stem radial growth varied across case studies, with growth occurring during the day and/or night, consistent with the available literature. Importantly, our approach provides a valuable alternative to existing methods, as it can be approximated by a simple empirical interpolation routine that derives irreversible radial growth using standard regression techniques. Our novel method provides an improved understanding of the relative source-sink carbon dynamics of tree stems at a sub-daily time scale. © 2016 The Authors Plant, Cell & Environment Published by John Wiley & Sons Ltd.

An empirical method that separates irreversible stem radial growth from bark water content changes in trees: theory and case studies

DOE PAGES

Mencuccini, Maurizio; Salmon, Yann; Mitchell, Patrick; ...

2017-11-12

Substantial uncertainty surrounds our knowledge of tree stem growth, with some of the most basic questions, such as when stem radial growth occurs through the daily cycle, still unanswered. Here, we employed high-resolution point dendrometers, sap flow sensors, and developed theory and statistical approaches, to devise a novel method separating irreversible radial growth from elastic tension-driven and elastic osmotically driven changes in bark water content. We tested this method using data from five case study species. Experimental manipulations, namely a field irrigation experiment on Scots pine and a stem girdling experiment on red forest gum trees, were used to validatemore » the theory. Time courses of stem radial growth following irrigation and stem girdling were consistent with a-priori predictions. Patterns of stem radial growth varied across case studies, with growth occurring during the day and/or night, consistent with the available literature. Importantly, our approach provides a valuable alternative to existing methods, as it can be approximated by a simple empirical interpolation routine that derives irreversible radial growth using standard regression techniques. In conclusion, our novel method provides an improved understanding of the relative source–sink carbon dynamics of tree stems at a sub-daily time scale.« less

Reengineering of MeSH thesauri for term selection to optimize literature retrieval and knowledge reconstruction in support of stem cell research.

PubMed

Su, Yan; Andrews, James; Huang, Hong; Wang, Yue; Kong, Liangliang; Cannon, Peter; Xu, Ping

2016-05-23

PubMed is a widely used database for scientists to find biomedical-related literature. Due to the complexity of the selected research subject and its interdisciplinary nature, as well as the exponential growth in the number of disparate pieces of biomedical literature, it is an overwhelming challenge for scientists to define the right search strategies and quickly locate all related information. Specialized subsets and groupings of controlled vocabularies, such as Medical Subject Headings (MeSH), can enhance information retrieval in specialized domains, such as stem cell research. There is a need to develop effective search strategies and convenient solutions for knowledge organization in stem cell research. The understanding of the interrelationships between these MeSH terms also facilitates the building of knowledge organization systems in related subject fields. This study collected empirical data for MeSH-related terms from stem cell literature and developed a novel approach that uses both automation and expert-selection to create a set of terms that supports enhanced retrieval. The selected MeSH terms were reconstructed into a classified thesaurus that can guide researchers towards a successful search and knowledge organization of stem cell literature. First, 4253 MeSH terms were harvested from a sample of 5527 stem cell related research papers from the PubMed database. Next, unrelated terms were filtered out based on term frequency and specificity. Precision and recall measures were used to help identify additional valuable terms, which were mostly non-MeSH terms. The study identified 15 terms that specifically referred to stem cell research for information retrieval, which would yield a higher precision (97.7 %) and recall (94.4 %) rates in comparison to other approaches. In addition, 128 root MeSH terms were selected to conduct knowledge organization of stem cell research in categories of anatomy, disease, and others. This study presented a novel strategy and procedure to reengineer term selections of the MeSH thesaurus for literature retrieval and knowledge organization using stem cell research as a case. It could help scientists to select their own search terms and build up a thesaurus-based knowledge organization system in interested and interdisciplinary research subject areas.

Potential of Induced Pluripotent Stem Cells (iPSCs) for Treating Age-Related Macular Degeneration (AMD).

PubMed

Fields, Mark; Cai, Hui; Gong, Jie; Del Priore, Lucian

2016-12-08

The field of stem cell biology has rapidly evolved in the last few decades. In the area of regenerative medicine, clinical applications using stem cells hold the potential to be a powerful tool in the treatment of a wide variety of diseases, in particular, disorders of the eye. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are promising technologies that can potentially provide an unlimited source of cells for cell replacement therapy in the treatment of retinal degenerative disorders such as age-related macular degeneration (AMD), Stargardt disease, and other disorders. ESCs and iPSCs have been used to generate retinal pigment epithelium (RPE) cells and their functional behavior has been tested in vitro and in vivo in animal models. Additionally, iPSC-derived RPE cells provide an autologous source of cells for therapeutic use, as well as allow for novel approaches in disease modeling and drug development platforms. Clinical trials are currently testing the safety and efficacy of these cells in patients with AMD. In this review, the current status of iPSC disease modeling of AMD is discussed, as well as the challenges and potential of this technology as a viable option for cell replacement therapy in retinal degeneration.

Phase contrast STEM for thin samples: Integrated differential phase contrast.

PubMed

Lazić, Ivan; Bosch, Eric G T; Lazar, Sorin

2016-01-01

It has been known since the 1970s that the movement of the center of mass (COM) of a convergent beam electron diffraction (CBED) pattern is linearly related to the (projected) electrical field in the sample. We re-derive a contrast transfer function (CTF) for a scanning transmission electron microscopy (STEM) imaging technique based on this movement from the point of view of image formation and continue by performing a two-dimensional integration on the two images based on the two components of the COM movement. The resulting integrated COM (iCOM) STEM technique yields a scalar image that is linear in the phase shift caused by the sample and therefore also in the local (projected) electrostatic potential field of a thin sample. We confirm that the differential phase contrast (DPC) STEM technique using a segmented detector with 4 quadrants (4Q) yields a good approximation for the COM movement. Performing a two-dimensional integration, just as for the COM, we obtain an integrated DPC (iDPC) image which is approximately linear in the phase of the sample. Beside deriving the CTFs of iCOM and iDPC, we clearly point out the objects of the two corresponding imaging techniques, and highlight the differences to objects corresponding to COM-, DPC-, and (HA) ADF-STEM. The theory is validated with simulations and we present first experimental results of the iDPC-STEM technique showing its capability for imaging both light and heavy elements with atomic resolution and a good signal to noise ratio (SNR). Copyright © 2015 Elsevier B.V. All rights reserved.

Arbuscular Mycorrhizal Fungi Increase the Phenolic Compounds Concentration in the Bark of the Stem of Libidibia Ferrea in Field Conditions

PubMed Central

dos Santos, Emanuela Lima; Alves da Silva, Francineyde; Barbosa da Silva, Fábio Sérgio

2017-01-01

Background: Libidibia ferrea is a species particular to the caatinga presenting medicinal properties for containing bioactive compounds. The use of Arbuscular Mycorrhizal Fungi (AMF) can increase the production of biomolecules in the legume leaves; however, no light has been shed on the role of symbiosis in maximizing metabolites production in the bark of L. ferrea stem. Objective: The aim was to select AMF that are efficient at increasing the production of phenolic compounds with medicinal properties in the bark of the L. ferrea stem. Methods: The experiment was designed in randomized blocks with four inoculation treatments (plants pre-inoculated with Claroideoglomus etunicatum, with Gigaspora albida, with Acaulospora longula, and non-inoculated plants – control) with six repetitions. Thirteen months after the transplanting, the plants were pruned and the bark of the stem was collected; subsequently, this plant material was dried in a chamber. After the drying process, fractions of the bark of the stem were macerated in methanol. The extracts were further used for analyses of the biomolecules. Results: The flavonoids concentration had an increase of, respectively, 236% and 186% in relation to the control for the treatments with A. longula and C. etunicatum; plants inoculated with A. longula had an increase of 47% in total tannins concentration compared with the non-inoculated control – a benefit that the proanthocyanidins did not present. Conclusion: Applying inoculation with A. longula may be an alternative to increase the production of biomolecules of the secondary metabolism in the bark of the L. ferrea stem in field conditions. PMID:29204223

The High School Environment and the Gender Gap in Science and Engineering

PubMed Central

Legewie, Joscha; DiPrete, Thomas A.

2016-01-01

Despite the striking reversal of the gender gap in education, women pursue science, technology, engineering, and mathematics (STEM) degrees at much lower rates than those of their male peers. This study extends existing explanations for these gender differences and examines the role of the high school context for plans to major in STEM fields. Building on recent gender theories, we argue that widely shared and hegemonic gender beliefs manifest differently across schools so that the gender-specific formation of study plans is shaped by the local environment of high schools. Using the National Education Longitudinal Study, we first show large variations between high schools in the ability to attract students to STEM fields conditional on a large set of pre–high school measures. Schools that are successful in attracting students to these fields reduce the gender gap by 25 percent or more. As a first step toward understanding what matters about schools, we then estimate the effect of two concrete high school characteristics on plans to major in STEM fields in college—a high school's curriculum in STEM and gender segregation of extracurricular activities. These factors have a substantial effect on the gender gap in plans to major in STEM: a finding that is reaffirmed in a number of sensitivity analyses. Our focus on the high school context opens concrete avenues for policy intervention and is of central theoretical importance to understand the gender gap in orientations toward STEM fields. PMID:27857451

The High School Environment and the Gender Gap in Science and Engineering.

PubMed

Legewie, Joscha; DiPrete, Thomas A

2014-10-01

Despite the striking reversal of the gender gap in education, women pursue science, technology, engineering, and mathematics (STEM) degrees at much lower rates than those of their male peers. This study extends existing explanations for these gender differences and examines the role of the high school context for plans to major in STEM fields. Building on recent gender theories, we argue that widely shared and hegemonic gender beliefs manifest differently across schools so that the gender-specific formation of study plans is shaped by the local environment of high schools. Using the National Education Longitudinal Study, we first show large variations between high schools in the ability to attract students to STEM fields conditional on a large set of pre-high school measures. Schools that are successful in attracting students to these fields reduce the gender gap by 25 percent or more. As a first step toward understanding what matters about schools, we then estimate the effect of two concrete high school characteristics on plans to major in STEM fields in college-a high school's curriculum in STEM and gender segregation of extracurricular activities. These factors have a substantial effect on the gender gap in plans to major in STEM: a finding that is reaffirmed in a number of sensitivity analyses. Our focus on the high school context opens concrete avenues for policy intervention and is of central theoretical importance to understand the gender gap in orientations toward STEM fields.

Using stem cell biology to study and treat ophthalmologic and oculoplastic diseases

PubMed Central

Wu, Albert Y.; Daniel, Michael G.

2017-01-01

With the rapid growth of the stem cell biology field, the prospect of regenerative medicine across multiple tissue types comes closer to reality. Several groundbreaking steps paved the way for applying stem cell biology to the several subfields within ophthalmology and oculoplastic surgery. These steps include the use of stem cell transplants as well as studies of various ophthalmologic pathologies at the molecular level. The necessity of stem cell transplant is readily apparent, having already been used for several studies such as artificial lacrimal gland design and eyelid reconstruction. Investigating the stem cell biology behind oncological diseases of the eye has also developed recently, such as with the identification of specific markers to label cancer stem cells in orbital adenoid cystic carcinoma. The advent of induced pluripotent stem cells led to a burst of productivity in the field of regenerative medicine, making it possible to take a patient's own cells, reprogram them, and use them to either study patient-specific pathology in vitro or use them for eventual patient specific therapeutics. Patient-specific adipose-derived stem cells (ASCs) have been used for a variety of treatments, such as wound healing and burn therapies. As the fields of stem cell biology and regenerative medicine continue to progress, its use will become a mainstay of patient-specific cell therapies in the future. PMID:29018761

A quantitative analysis of factors that influence and predict students' intention to major in and complete an undergraduate program in STEM or non-STEM

NASA Astrophysics Data System (ADS)

Yang, Xuemei

2005-11-01

The goal of this study was to explore and understand the factors that influence students' intention to major in and complete an undergraduate program in a science, technology, engineering, or mathematics (STEM) discipline, in a non-STEM field, and how students' gender directly and indirectly affects their success in college. A quantitative study of three thousand four (3004) ACT-tested students who entered a Midwestern, land-grant university as freshmen in fall, 1999 was conducted based on their ACT Assessment information and their enrollment and graduation status after five years. A wide variety of variables were considered and logistic regression, factor analysis, and path analysis were used to analyze the data. The results show that students who intended to major in or completed STEM programs generally have better academic qualifications than their counterparts who intended to major in non-STEM fields. Students who intended to major in or completed STEM programs came from lower income families and smaller communities than those who intended to major in or graduated from non-STEM programs. In this study, gender's direct effect on students' college achievement is eleven times the total of gender's indirect effects through several major factors for students in both STEM fields and non-STEM fields. Perhaps nature has favored females when students' achievement is measured as their college GPA. The results also show that the overall high dropout rate is strongly associated with students' inadequate preparation in high school and family income. Out-of-school accomplishment in community service is a negative influence on their completion of a college degree. ACT scores are not necessary for prediction of college graduation.

A Research about the Placement of the Top Thousand Students in STEM Fields in Turkey between 2000 and 2014

ERIC Educational Resources Information Center

Akgunduz, Devrim

2016-01-01

STEM (Science, Technology, Engineering and Mathematics), one of the mostly emphasized concepts in the world, is a paradigm that creates interdisciplinary learning and provides achievement of the outcomes of science, mathematics, engineering and technology while doing this. This research was carried out to investigate the STEM fields' placement of…

The Threat of Sexism in a STEM Educational Setting: The Moderating Impacts of Ethnicity and Legitimacy Beliefs on Test Performance

ERIC Educational Resources Information Center

O'Brien, Laurie T.; Garcia, Donna M.; Adams, Glenn; Villalobos, J. Guillermo; Hammer, Elliott; Gilbert, Patricia

2015-01-01

Social identity threat has negative consequences for women in science, technology, engineering, and mathematics (STEM) fields. The present study examined whether legitimacy beliefs--beliefs that status differences between men and women in STEM fields are fair--put women at risk for experiencing social identity threat and poorer performance on a…

Early Urban Field Experiences for Prospective Teachers: A Case Study of Multicultural Field Placements through a University-Based Preservice STEM Teacher Program

ERIC Educational Resources Information Center

Segal, Eden H.

2011-01-01

Educational and political leaders have expressed concern about racial and ethnic disparities in students' readiness for postsecondary study and careers in science, technology, engineering, and mathematics (STEM). A lack of preparedness of STEM teachers in high-need urban districts, which serve predominantly low-income minority students, is often…

STEM Education: Attracting and Retaining Female Students in Secondary STEM Programs

ERIC Educational Resources Information Center

Ruff, Zachary A.

2017-01-01

This narrative case study examines a high achieving STEM based high school and its ability to attract, retains, and engage female students. Given the recent importance placed on STEM graduates and STEM careers it is important for schools to understand how they can engage traditionally underserved minorities in STEM fields. The research used a…

Federal STEM Policy and Politics and Their Impact on Astronomy EPO: Reflections and Provocations

NASA Astrophysics Data System (ADS)

Schultz, G.; Storksdieck, M.; Canright, S.

2015-11-01

The federal government invests more than $3 billion each year across its various units in supporting STEM education and outreach. Efforts in recent years to understand and better coordinate these investments have resulted in considerable pushback, particularly those efforts that aimed at consolidation and elimination of programs deemed ineffective or duplicative. While initial plans to streamline federal STEM education were defeated, many agencies nonetheless saw cuts and elimination, and a high-level effort to coordinate STEM education at the cross-agency level is now gaining steam (CoSTEM: Committee on Science, Technology, Engineering, and Mathematics Education). What do all of these developments mean for education and public outreach in astronomy and related fields? How should this community operate within the opportunities and threats that CoSTEM might pose? Former director of the National Academy of Science's Board on Science Education, and now director of the Center for Research on Lifelong STEM Learning, Martin Storksdieck, reflected on past and recent developments from the perspective of a close observer, and from the perspective of someone who has been involved in astronomy education research and evaluation for nearly 20 years. Shelley Canright, Senior Advisor for Education Integration at the NASA Office of Education, shared her insights and perspectives with respect to CoSTEM and EPO, in particular from co-chairing the Federal Coordination in Science, Technology, Engineering, and Mathematics Education (FC-STEM) group.

High-order above-threshold photoemission from nanotips controlled with two-color laser fields

NASA Astrophysics Data System (ADS)

Seiffert, Lennart; Paschen, Timo; Hommelhoff, Peter; Fennel, Thomas

2018-07-01

We investigate the process of phase-controlled high-order above-threshold photoemission from metallic nanotips under bichromatic laser fields. Experimental photoelectron spectra resulting from two-color excitation with a moderately intense near-infrared fundamental field (1560 nm) and its weak second harmonic show a strong sensitivity on the relative phase and clear indications for a plateau-like structure that is attributed to elastic backscattering. To explore the relevant control mechanisms, characteristic features, and particular signatures from the near-field inhomogeneity, we performed systematic quantum simulations employing a one-dimensional nanotip model. Besides rich phase-dependent structures in the simulated above-threshold ionization photoelectron spectra we find ponderomotive shifts as well as substantial modifications of the rescattering cutoff as function of the decay length of the near-field. To explore the quantum or classical nature of the observed features and to discriminate the two-color effects stemming from electron propagation and from the ionization rate we compare the quantum results to classical trajectory simulations. We show that signatures from direct electrons as well as the modulations in the plateau region mainly stem from control of the ionization probability, while the modulation in the cutoff region can only be explained by the impact of the two-color field on the electron trajectory. Despite the complexity of the phase-dependent features that render two-color strong-field photoemission from nanotips intriguing for sub-cycle strong-field control, our findings support that the recollision features in the cutoff region provide a robust and reliable method to calibrate the relative two-color phase.

STEM development: A study of 6th--12th grade girls' interest and confidence in mathematics and science

NASA Astrophysics Data System (ADS)

Heaverlo, Carol Ann

Researchers, policymakers, business, and industry have indicated that the United States will experience a shortage of professionals in the Science, Technology, Engineering, and Mathematics (STEM) fields. Several strategies have been suggested to address this shortage, one of which includes increasing the representation of girls and women in the STEM fields. In order to increase the representation of women in the STEM fields, it is important to understand the developmental factors that impact girls' interest and confidence in STEM academics and extracurricular programs. Research indicates that greater confidence leads to greater interest and vice versa (Denissen et al., 2007). This study identifies factors that impact girls' interest and confidence in mathematics and science, defined as girls' STEM development. Using Bronfenbrenner's (2005) bioecological model of human development, several factors were hypothesized as having an impact on girls' STEM development; specifically, the macrosystems of region of residence and race/ethnicity, and the microsystems of extracurricular STEM activities, family STEM influence, and math/science teacher influence. Hierarchical regression analysis results indicated that extracurricular STEM involvement and math teacher influence were statistically significant predictors for 6--12th grade girls' interest and confidence in mathematics. Furthermore, hierarchical regression analysis results indicated that the only significant predictor for 6--12th grade girls' interest and confidence in science was science teacher influence. This study provides new knowledge about the factors that impact girls' STEM development. Results can be used to inform and guide educators, administrators, and policy makers in developing programs and policy that support and encourage the STEM development of 6--12th grade girls.

Increasing College Students' Interest and Engagement in STEM: A Comparison of Strategies for Challenging STEM Stereotypes

NASA Astrophysics Data System (ADS)

Shin, Jiyun Elizabeth L.

Increasing science, technology, engineering, and mathematics (STEM) graduates has become an important part of the education agenda in the U.S. in recent years. Stereotypes about STEM (i.e., belief that STEM abilities are innate, and that European American men are best suited for STEM) have been identified as one of the critical factors that may contribute to low recruitment and retention of STEM students. Drawing from the literatures on biological essentialism and role models, this study compared different strategies for challenging STEM stereotypes among undergraduate students in STEM and non-STEM fields. STEM stereotypes were challenged directly with research articles that provided non-biological explanations for STEM success and interest (a strategy used in the essentialism research) and indirectly with biographies of successful STEM role models who are underrepresented in their field and who succeeded through hard work (a strategy used in the role model research). Contrary to the predictions, exposure to the role model biographies, research articles, or combination of both did not have statistically significant effects on participants' reported STEM interest and academic intentions. Possible explanations for the lack of significant findings as well as suggestions for developing effective interventions to promote STEM engagement among students are discussed.

The Perceptions of STEM from Eighth-Grade African-American Girls in a High-Minority Middle School

NASA Astrophysics Data System (ADS)

Hare, LaChanda N.

Even with the existence of STEM curriculum and STEM programs that target women and minorities, African-American females still lag behind other ethnic groups in STEM fields. Reasons for the underrepresentation of females in STEM fields can be traced back to the early years of schooling. The purpose of this study was to identify the factors that impact African-American females' perspectives of STEM subjects and STEM careers. An explanatory sequential mixed-methods approach was used for data collection with a survey, focus group, and interview. Forty male (N=12) and female (N=28) students from different ethnic groups were surveyed. The focus group and interview sessions consisted of 21 African-American females from two distinct groups: those enrolled in the school's STEM program (STEM) and those who were not enrolled in the STEM program (Non-STEM). The self-efficacy theory and social cognitive career theory served as the theoretical constructs guiding the data analysis. Multiple regression results showed that outcome expectation and personal disposition had the greatest influence on the females' interest in STEM content and STEM careers. Results from the qualitative portion of the study revealed that the learning environment and STEM self-efficacy had a significant impact on African-American females' interest in STEM.

Influence of near null magnetic field on in vitro growth of potato and wild Solanum species.

PubMed

Rakosy-Tican, Lenuta; Aurori, C M; Morariu, V V

2005-10-01

The influence of near null magnetic field on in vitro growth of different cultures of potato and related Solanum species was investigated for various exposure times and dates. Potato (Solanum tuberosum L. cv. Désirée) in vitro cultures of shoot tips or nodal segments were used. Three different exposure periods revealed either stimulation or inhibition of root, stem, or leaf in vitro growth after 14 or 28 days of exposure. In one experiment the significant stimulation of leaf growth was also demonstrated at biochemical level, the quantity of chlorophyll a and b and carotenoids increasing more than two-fold. For the wild species Solanum chacoense, S. microdontum, and S. verrucosum, standardized in vitro cultures of nodal stem segments were used. Root and stem growth was either stimulated or slightly inhibited after 9 days exposure to near null magnetic field. Callus cultures obtained from potato dihaploid line 120/19 were maintained in near null magnetic field in 2 different months. For these experiments as well as for Solanum verrucosum, callus cultures recorded either slight inhibition or no effect on fresh weight. For all experiments significant growth variation was brought about only when geomagnetic activity (AP index) showed variations at the beginning of in vitro growth and when the explant had at least one meristematic tissue. Moreover longer maintenance in near null magnetic field, 28 days as compared to 14 days or the controls, can also make a difference in plant growth in response to geomagnetic field variations when static component was reduced to zero value. These results of in vitro plant growth stimulation by variable component of geomagnetic field also sustain the so-called seasonal "window" effect. (c) 2005 Wiley-Liss, Inc.

STEM Education for Girls of Color

NASA Astrophysics Data System (ADS)

Yee, Kam H.

Science, technology, engineering, and math (STEM) fields struggle to increase recruitment and retention of girls of color. The dominant framework in STEM education is the pipeline which assumes girls in general lack motivation and interest to persist in STEM fields. Recent public discourse shifts to address institutionalized discrimination and systemic barriers in STEM culture that filter out underrepresented populations. Informal education or complementary learning STEM programs offer alternative opportunities for students to explore outside of rigid school academic and social systems. Few articles look specifically at STEM complementary learning programs, and even fewer focus on the effects on girls of color. This research is a quantitative study to categorize existing mission statements and training behind organizations that provide STEM programs. The results will provide a better understanding of the relationship between practices of STEM education organizations and the programs they create. Diversity training and inclusive language in mission statements had weak correlations with increased cultural responsiveness in the program offerings. The results suggest organizations must be more intentional and explicit when implementing diversity goals.

Gendered transitions to adulthood by college field of study in the United States

PubMed Central

Han, Siqi; Tumin, Dmitry; Qian, Zhenchao

2017-01-01

BACKGROUND Field of study may influence the timing of transitions to the labor market, marriage, and parenthood among college graduates. Research to date has yet to study how field of study is associated with the interweaving of these transitions in the USA. OBJECTIVE The current study examines gendered influences of college field of study on transitions to a series of adult roles, including full-time work, marriage, and parenthood. METHODS We use Cox proportional hazards models and multinomial logistic regression to examine gendered associations between field of study and the three transitions among college graduates of the NLSY97 (National Longitudinal Survey of Youth) cohort. RESULTS Men majoring in STEM achieve early transitions to full-time work, marriage, and parenthood; women majoring in STEM show no significant advantage in finding full-time work and delayed marriage and childbearing; women in business have earlier transitions to full-time work and marriage than women in other fields, demonstrating an advantage similar to that of men in STEM. CONCLUSIONS The contrast between men and women in STEM shows that transition to adulthood remains gendered; the contrast between women in STEM and women in business illustrates that a prestigious career may not necessarily delay family formation. PMID:29075146

Scanning transmission electron microscopy methods for the analysis of nanoparticles.

PubMed

Ponce, Arturo; Mejía-Rosales, Sergio; José-Yacamán, Miguel

2012-01-01

Here we review the scanning transmission electron microscopy (STEM) characterization technique and STEM imaging methods. We describe applications of STEM for studying inorganic nanoparticles, and other uses of STEM in biological and health sciences and discuss how to interpret STEM results. The STEM imaging mode has certain benefits compared with the broad-beam illumination mode; the main advantage is the collection of the information about the specimen using a high angular annular dark field (HAADF) detector, in which the images registered have different levels of contrast related to the chemical composition of the sample. Another advantage of its use in the analysis of biological samples is its contrast for thick stained sections, since HAADF images of samples with thickness of 100-120 nm have notoriously better contrast than those obtained by other techniques. Combining the HAADF-STEM imaging with the new aberration correction era, the STEM technique reaches a direct way to imaging the atomistic structure and composition of nanostructures at a sub-angstrom resolution. Thus, alloying in metallic nanoparticles is directly resolved at atomic scale by the HAADF-STEM imaging, and the comparison of the STEM images with results from simulations gives a very powerful way of analysis of structure and composition. The use of X-ray energy dispersive spectroscopy attached to the electron microscope for STEM mode is also described. In issues where characterization at the atomic scale of the interaction between metallic nanoparticles and biological systems is needed, all the associated techniques to STEM become powerful tools for the best understanding on how to use these particles in biomedical applications.

Understanding the Factors that Support the Use of Active Learning Teaching in STEM Undergraduate Courses: Case Studies in the Field of Geoscience

NASA Astrophysics Data System (ADS)

Iverson, Ellen A. Roscoe

The purpose of this study was to understand the factors that support the adoption of active learning teaching strategies in undergraduate courses by faculty members, specifically in the STEM disciplines related to geoscience. The focus of the study centered on the context of the department which was identified as a gap in evaluation and educational research studies of STEM faculty development. The study used a mixed-method case study methodology to investigate the influences of departmental context on faculty members' adoption of active-learning teaching practices. The study compared and contrasted the influence of two faculty development strategies initiated in the field of geoscience. Six university geoscience departments were selected that had participated in two national geoscience professional development programs. Data were generated from 19 faculty interviews, 5 key informant interviews, and documents related to departmental and institutional context. The study concluded that two main factors influenced the degree to which faculty who participated in geoscience faculty development reported adoption of active learning pedagogies. These conclusions are a) the opportunity to engage in informal, regular conversations with departmental colleagues about teaching promoted adoption of new teaching approaches and ideas and b) institutional practices regarding the ways in which teaching practices were typically measured, valued, and incentivized tended to inhibit risk taking in teaching. The conclusions have implications related to institutional policy, faculty development, and the role of evaluation.

Bluegill growth as modified by plant density: an exploration of underlying mechanisms

USGS Publications Warehouse

Savino, Jacqueline F.; Marschall, Elizabeth A.; Stein, Roy A.

1992-01-01

Bluegill (Lepomis macrochira) growth varies inconsistently with plant density. In laboratory and field experiments, we explored mechanisms underlying bluegill growth as a function of plant and invertebrate density. In the laboratory, bluegills captured more chironomids (Chironomus riparius) than damselflies (Enallagma spp. and Ischnura spp.), but energy intake per time spent searching did not differ between damselfly and chironomid treatments. From laboratory data, we described prey encounter rates as functions of plant and invertebrate density. In Clark Lake, Ohio, we created 0.05-ha mesocosms of inshore vegetation to generate macrophyte densities of 125, 270, and 385 stems/m2 of Potamogeton and Ceratophyllum and added 46-mm bluegill (1/m2). In these mesocosms, invertebrate density increased as a function of macrophyte density. Combining this function with encounter rate functions derived from laboratory data, we predicted that bluegill growth should peak at a high macrophyte density, greater than 1000 stems/m2, even though growth should change only slightly beyond 100 stems/m2. Consistent with our predictions, bluegills did not grow differentially, nor did their use of different prey taxa differ, across macrophyte densities in the field. Bluegills preferred chironomid pupae, which were relatively few in numbers but vulnerable to predation, whereas more cryptic, chironomid larvae, which were associated with vegetation but were relatively abundant, were eaten as encountered. Bluegill avoided physid snails. Contrary to previous work, vegetation did not influence growth or diet of bluegill beyond relatively low densities owing to the interaction between capture probabilities and macroinvertebrate densities.

The Effect of STEM Learning through the Project of Designing Boat Model toward Student STEM Literacy

NASA Astrophysics Data System (ADS)

Tati, T.; Firman, H.; Riandi, R.

2017-09-01

STEM Learning focusses on development of STEM-literate society, the research about implementation of STEM learning to develope students’ STEM literacy is still limited. This study is aimed to examine the effect of implementation STEM learning through the project of designing boat model on students STEM literacy in energy topic. The method of this study was a quasi-experiment with non-randomized pretest-posttest control group design. There were two classes involved, the experiment class used Project Based Learning with STEM approach and control class used Project-Based Learning without STEM approach. A STEM Literacy test instrument was developed to measure students STEM literacy which consists of science literacy, mathematics literacy, and technology-engineering literacy. The analysis showed that there were significant differences on improvement science literacy, mathematics technology-engineering between experiment class and control class with effect size more than 0.8 (large effect). The difference of improvement of STEM literacy between experiment class and control class is caused by the existence of design engineering activity which required students to apply the knowledge from every field of STEM. The challenge that was faced in STEM learning through design engineering activity was how to give the students practice to integrate STEM field in solving the problems. In additional, most of the students gave positive response toward implementation of STEM learning through design boat model project.

Design of magnetic gene complexes as effective and serum resistant gene delivery systems for mesenchymal stem cells.

PubMed

Zhang, Tian-Yuan; Wu, Jia-He; Xu, Qian-Hao; Wang, Xia-Rong; Lu, Jingxiong; Hu, Ying; Jo, Jun-Ichiro; Yamamoto, Masaya; Ling, Daishun; Tabata, Yasuhiko; Gao, Jian-Qing

2017-03-30

Gene engineered mesenchymal stem cells (MSCs) have been proposed as promising tools for their various applications in biomedicine. Nevertheless, the lack of an effective and safe way to genetically modify these stem cells is still a major obstacle in the current studies. Herein, we designed novel magnetic complexes by assembling cationized pullulan derivatives with magnetic iron oxide nanoparticles for delivering target genes to MSCs. Results showed that this complexes achieved effective gene expression with the assistance of external magnetic field, and resisted the adverse effect induced by serum proteins on the gene delivery. Moreover, neither significant cytotoxicity nor the interference on the osteogenic differentiation to MSCs were observed after magnetofection. Further studies revealed that this effective and serum resistant gene transfection was partly due to the accelerated and enhanced intracellular uptake process driven by external magnetic field. To conclude, the current study presented a novel option for genetic modification of MSCs in an effective, relatively safe and serum compatible way. Copyright © 2017 Elsevier B.V. All rights reserved.

Determining oxygen relaxations at an interface: A comparative study between transmission electron microscopy techniques.

PubMed

Gauquelin, N; van den Bos, K H W; Béché, A; Krause, F F; Lobato, I; Lazar, S; Rosenauer, A; Van Aert, S; Verbeeck, J

2017-10-01

Nowadays, aberration corrected transmission electron microscopy (TEM) is a popular method to characterise nanomaterials at the atomic scale. Here, atomically resolved images of nanomaterials are acquired, where the contrast depends on the illumination, imaging and detector conditions of the microscope. Visualization of light elements is possible when using low angle annular dark field (LAADF) STEM, annular bright field (ABF) STEM, integrated differential phase contrast (iDPC) STEM, negative spherical aberration imaging (NCSI) and imaging STEM (ISTEM). In this work, images of a NdGaO 3 -La 0.67 Sr 0.33 MnO 3 (NGO-LSMO) interface are quantitatively evaluated by using statistical parameter estimation theory. For imaging light elements, all techniques are providing reliable results, while the techniques based on interference contrast, NCSI and ISTEM, are less robust in terms of accuracy for extracting heavy column locations. In term of precision, sample drift and scan distortions mainly limits the STEM based techniques as compared to NCSI. Post processing techniques can, however, partially compensate for this. In order to provide an outlook to the future, simulated images of NGO, in which the unavoidable presence of Poisson noise is taken into account, are used to determine the ultimate precision. In this future counting noise limited scenario, NCSI and ISTEM imaging will provide more precise values as compared to the other techniques, which can be related to the mechanisms behind the image recording. Copyright © 2017 Elsevier B.V. All rights reserved.

Science Fiction Exhibits as STEM Gateways

NASA Astrophysics Data System (ADS)

Robie, Samantha

Women continue to hold less than a quarter of all STEM jobs in the United States, prompting many museums to develop programs and exhibits with the express goal of interesting young girls in scientific fields. At the same time, a number of recent museum exhibits have harnessed the popularity of pop culture and science fiction in order to interest general audiences in STEM subject matter, as well as using the exhibits as springboards to expand or shift mission goals and focus. Because science fiction appears to be successful at raising interest in STEM fields, it may be an effective way to garner the interest of young girls in STEM in particular. This research seeks to describe the ways in which museums are currently using science fiction exhibits to interest young girls in STEM fields and careers. Research focused on four institutions across the country hosting three separate exhibits, and included staff interviews and content analysis of exhibit descriptions, promotional materials, a summative evaluation and supplementary exhibit productions. In some ways, science fiction exhibits do serve young girls, primarily through the inclusion of female role models, staff awareness, and prototype testing to ensure interactives are attractive to girls as well as to boys. However, STEM appears to be underutilized, which may be partly due to a concern within the field that the outcome of targeting a specific gender could be construed as "stereotyping".

Quality of evidence revealing subtle gender biases in science is in the eye of the beholder.

PubMed

Handley, Ian M; Brown, Elizabeth R; Moss-Racusin, Corinne A; Smith, Jessi L

2015-10-27

Scientists are trained to evaluate and interpret evidence without bias or subjectivity. Thus, growing evidence revealing a gender bias against women-or favoring men-within science, technology, engineering, and mathematics (STEM) settings is provocative and raises questions about the extent to which gender bias may contribute to women's underrepresentation within STEM fields. To the extent that research illustrating gender bias in STEM is viewed as convincing, the culture of science can begin to address the bias. However, are men and women equally receptive to this type of experimental evidence? This question was tested with three randomized, double-blind experiments-two involving samples from the general public (n = 205 and 303, respectively) and one involving a sample of university STEM and non-STEM faculty (n = 205). In all experiments, participants read an actual journal abstract reporting gender bias in a STEM context (or an altered abstract reporting no gender bias in experiment 3) and evaluated the overall quality of the research. Results across experiments showed that men evaluate the gender-bias research less favorably than women, and, of concern, this gender difference was especially prominent among STEM faculty (experiment 2). These results suggest a relative reluctance among men, especially faculty men within STEM, to accept evidence of gender biases in STEM. This finding is problematic because broadening the participation of underrepresented people in STEM, including women, necessarily requires a widespread willingness (particularly by those in the majority) to acknowledge that bias exists before transformation is possible.

Design and development of a magnetic device for mesenchymal stem cell retaining in deep targets

NASA Astrophysics Data System (ADS)

Banis, G. C.

2017-12-01

This paper focuses on the retaining of mesenchymal stem cells in blood flow conditions using the appropriate magnetic field. Mesenchymal stem cells can be tagged with magnetic nanoparticles and thus, they can be manipulated from distance, through the application of an external magnetic field. In this paper the case of kidney as target of the therapy is being studied.

What's Life Got to Do with It? The Role of Life Experiences in Shaping Female Community College Students' Transfer Intent in STEM Fields of Study

ERIC Educational Resources Information Center

Wickersham, Kelly; Wang, Xueli

2016-01-01

Transfer in science, technology, engineering, and math (STEM) fields from community colleges to 4-year institutions holds great policy significance in alleviating the female underrepresentation in the STEM pipeline, with proportionately more female students attending community colleges. Considering the knowledge gap on this often overlooked topic,…

Factors That Female Higher Education Faculty in Select Science, Technology, Engineering, and Mathematics (STEM) Fields Perceive as Being Influential to Their Success and Persistence in Their Chosen Professions

ERIC Educational Resources Information Center

Opare, Phyllis Bernice

2012-01-01

The purpose of this study was to determine factors female higher education faculty in select science, technology, engineering, and mathematics (STEM) fields perceived as influential to their success and persistence in their chosen professions. Females are underrepresented in STEM professions including academia, despite the fact that female…

Curbing stem cell tourism in South Africa.

PubMed

Meissner-Roloff, Madelein; Pepper, Michael S

2013-12-01

Stem cells have received much attention globally due in part to the immense therapeutic potential they harbor. Unfortunately, malpractice and exploitation (financial and emotional) of vulnerable patients have also drawn attention to this field as a result of the detrimental consequences experienced by some individuals that have undergone unproven stem cell therapies. South Africa has had limited exposure to stem cells and their applications and, while any exploitation is detrimental to the field of stem cells, South Africa is particularly vulnerable in this regard. The current absence of adequate legislation and the inability to enforce existing legislation, coupled to the sea of misinformation available on the Internet could lead to an increase in illegitimate stem cell practices in South Africa. Circumstances are already precarious because of a lack of understanding of concepts involved in stem cell applications. What is more, credible and easily accessible information is not available to the public. This in turn cultivates fears born out of existing superstitions, cultural beliefs, rituals and practices. Certain cultural or religious concerns could potentially hinder the effective application of stem cell therapies in South Africa and novel ways of addressing these concerns are necessary. Understanding how scientific progress and its implementation will affect each individual and, consequently, the community, will be of cardinal importance to the success of the fields of stem cell therapy and regenerative medicine in South Africa. A failure to understand the ethical, cultural or moral ramifications when new scientific concepts are introduced could hinder the efficacy and speed of bringing discoveries to the patient. Neglecting proper procedure for establishing the field would lead to long delays in gaining public support in South Africa. Understanding the dangers of stem cell tourism - where vulnerable patients are subjected to unproven stem cell therapies that have not undergone peer review or been registered with the relevant local authorities - becomes imperative so that strategies to overcome this threat can be implemented.

Short-term, informal, and low-stakes scientific laboratory and field experiences improve STEM student retention and academic success

NASA Astrophysics Data System (ADS)

Hintz, C.; Pride, C. J.; Cox, T.

2017-12-01

Formal internship experiences strongly improve student success in the STEM fields. Classical programs like NSF's Research Experiences for Undergraduates are highly successful for traditional and non-traditional students. Moreover when early undergraduate and at-risk (e.g., low income, academically-challenged) students engage in these experiences, their career paths are re-enforced or changed, academic progress and retention improves, and they are encouraged to continue into graduate school. Students build connections to their course-based learning and experience the life of a working scientist. However, NSF formal experiences are relatively expensive to provide (>5000 per student per experience) and are available to fewer than 5% of geoscience majors each year. Although other funded formal internship opportunities exist, they are likely available to no more than 10% of total enrolled geoscience students. These high-quality programs cannot impact enough early undergraduate students to encourage their remaining in science and improve the current overall retention and graduation rates in the US. Savannah State University faculty successfully completed multiple grants funding low-stakes undergraduate field-science experiences. These short-term (semester to year), part-time (5-10h/week) experiences provide similar classroom-to-real-world science connections, offer students direct laboratory and field experiences, build skill sets, and provide a small source of revenue assisting financially-challenged students to stay on campus rather than seeking off-campus employment. For a much lower investment in time and grant resources (500-1500 per student per experience), participant graduation rates exceeded 80%, well above the university 27-34% graduation rate during the same time period. Relatively small infusions of research dollars targeting undergraduate experiences in the field and laboratory could significantly impact long-term student outcomes in STEM disciplines. These findings supported by NSF OCE-1460457, OCE-1156525, GEO-0914680; ONR N00014-12-1-0969.

Making stem cells count for global health.

PubMed

McMahon, Dominique S; Thorsteinsdóttir, Halla

2011-11-01

Developing countries such as China, India and Brazil are making large investments in the stem cell field. Here we argue that hands-on involvement in the field by these countries is essential if the products developed are going to be locally relevant, affordable and appropriate. However, stem cells are a high-risk investment and any global health impacts are still likely to be far off. Even if they are eventually successful, better clinical oversight and measures to ensure access are required for stem cells to have a substantial and equitable impact.

Adolescent girls' experiences and gender-related beliefs in relation to their motivation in math/science and english.

PubMed

Leaper, Campbell; Farkas, Timea; Brown, Christia Spears

2012-03-01

Although the gender gap has dramatically narrowed in recent decades, women remain underrepresented in many science, technology, engineering, and mathematics (STEM) fields. This study examined social and personal factors in relation to adolescent girls' motivation in STEM (math/science) versus non-STEM (English) subjects. An ethnically diverse sample of 579 girls ages 13-18 years (M = 15) in the U.S. completed questionnaires measuring their academic achievement, ability beliefs, values, and experiences. Social and personal factors were hypothesized to predict motivation (expectancy-value) differently in math/science (M/S) and English. Social factors included perceived M/S and English support from parents and peers. Personal factors included facets of gender identity (felt conformity pressure, gender typicality, gender-role contentedness), gender-related attitudes, and exposure to feminism. In addition, grades, age, parents' education, and ethnicity were controlled. Girls' M/S motivation was positively associated with mother M/S support, peer M/S support, gender-egalitarian beliefs, and exposure to feminism; it was negatively related to peer English support. Girls' English motivation was positively associated with peer English support as well as felt pressure from parents; it was negatively related to peer M/S support and felt peer pressure. The findings suggest that social and personal factors may influence girls' motivation in domain-specific ways.

Personalized Regenerative Medicine.

PubMed

Arjmand, Babak; Goodarzi, Parisa; Mohamadi-Jahani, Fereshteh; Falahzadeh, Khadijeh; Larijani, Bagher

2017-03-01

Personalized medicine as a novel field of medicine refers to the prescription of specific therapeutics procedure for an individual. This approach has established based on pharmacogenetic and pharmacogenomic information and data. The terms precision and personalized medicines are sometimes applied interchangeably. However, there has been a shift from "personalized medicine" towards "precision medicine". Although personalized medicine emerged from pharmacogenetics, nowadays it covers many fields of healthcare. Accordingly, regenerative medicine and cellular therapy as the new fields of medicine use cell-based products in order to develop personalized treatments. Different sources of stem cells including mesenchymal stem cells, embryonic stem cells and induced pluripotent stem cells (iPSCs) have been considered in targeted therapies which could give many advantages. iPSCs as the novel and individual pluripotent stem cells have been introduced as the appropriate candidates for personalized cell therapies. Cellular therapies can provide a personalized approach. Because of person-to-person and population differences in the result of stem cell therapy, individualized cellular therapy must be adjusted according to the patient specific profile, in order to achieve best therapeutic results and outcomes. Several factors should be considered to achieve personalized stem cells therapy such as, recipient factors, donor factors, and the overall body environment in which the stem cells could be active and functional. In addition to these factors, the source of stem cells must be carefully chosen based on functional and physical criteria that lead to optimal outcomes.

The Relationship between Self-Efficacy and Advanced STEM Coursework in Female Secondary Students

ERIC Educational Resources Information Center

Bernasconi, Bethany

2017-01-01

Despite years of attention, gender inequity persists in science, technology, engineering, and mathematics (STEM). Female STEM faculty, positive social interactions, and enrollment in advanced STEM secondary coursework are supportive factors in promoting female students' persistence in STEM fields. To address the gap in understanding these factors,…

Effect of nitrogen fertilization and fungicides on Botryosphaeria stem blight lesion development on detached stems

USDA-ARS?s Scientific Manuscript database

: Botryosphaeria stem blight is a destructive disease of blueberries that has not been well managed with fungicides. Field observations showed that stem blight is more severe on vigorously growing plants than on slower growing plants. Detached stem assays were used to compare the effect of fertil...

Workforce and graduate school outcomes of NOAA's Educational Partnership Program

NASA Astrophysics Data System (ADS)

Christenson, T.; Kaplan, M.

2017-12-01

Underrepresented groups, including Black, Hispanic, Native American, Alaska Native, Native Hawaiian and Pacific Island professionals remain underrepresented in STEM fields generally, and in the ocean and atmospheric sciences specifically. NOAA has tried to address this disparity through a number of initiatives under the Educational Partnership Program with Minority Serving Institutions (EPP MSI) which currently has two components: four Cooperative Science Centers (CSCs) aligned with NOAA's mission areas; and an Undergraduate Scholarship Program (USP), both established in 2001. In order to determine the outcomes for the program participants and the impacts of these programs on degree completions and on the workforce, the EPP MSI undertook a multi-pronged effort to identify career and education achievements for 80% of the approximately 1750 EPP MSI alumni, 75% of whom are from underrepresented groups. This was accomplished through 1) searching online resources (e.g. professional web pages, LinkedIn, etc.), 2) personal communication with program-associated faculty, 3) National Student Clearinghouse, 4) a survey of former scholars conducted by Insight Policy Research, and 5) self-reporting though NOAA's Voluntary Alumni Update System. Results show that 60% of CSC alumni currently hold an advanced degree in a STEM field with another 8% currently working toward one. 66% of EPP Undergraduate Scholars go to graduate school. 72% of CSC and USP alumni are currently employed in or pursuing a graduate degree in a NOAA-related* field. More than 70 CSC graduates currently work for NOAA as contractors or federal employees while more than 240 work for other government agencies. More than 400 are employed in the private sector. Of more than 225 PhD graduates, 66 have completed or currently hold post-doctoral positions in NOAA mission fields; 71 have held faculty positions at major universities. However, one challenge is retaining diverse STEM talent within the Geosciences in light of the lure of lucrative jobs in other STEM fields and ensuring robust outcomes beyond degree completions.

How can ethics relate to science? The case of stem cell research

PubMed Central

Carvalho, Ana Sofia; Ramalho-Santos, João

2013-01-01

We live in an era of an important turning point in the relationship between ethics (or, more accurately, bioethics) and science, notably due to both public interest and the gradual tightening of the gap in time between scientific discoveries and ethical reflection. The current bioethics debates of emerging situations (pluripotent stem cells, gene therapy, nanotechnology) have undoubtedly contributed to this change. Today, science happens and bioethics reflects on the possibilities, considers the risks, and advances proposals, which, without being scientific, can also imprint a mark on the path of scientific development. In this article, through the narrative of stem cell research, we will try to illustrate how bringing a bioethical viewpoint to the scientific debate can become a healthy exercise in both ethics and science, especially as narratives shift, as was the case in this field due to the introduction of induced pluripotent stem cells, the advent of which is not easily dissociated from the controversies related to embryo research. We should perhaps welcome this trend as promising for the future relationship between ethics and scientific research, providing a stimulus (and not a block) to the ever-evolving scientific discourse. PMID:23150079

How can ethics relate to science? The case of stem cell research.

PubMed

Carvalho, Ana Sofia; Ramalho-Santos, João

2013-06-01

We live in an era of an important turning point in the relationship between ethics (or, more accurately, bioethics) and science, notably due to both public interest and the gradual tightening of the gap in time between scientific discoveries and ethical reflection. The current bioethics debates of emerging situations (pluripotent stem cells, gene therapy, nanotechnology) have undoubtedly contributed to this change. Today, science happens and bioethics reflects on the possibilities, considers the risks, and advances proposals, which, without being scientific, can also imprint a mark on the path of scientific development. In this article, through the narrative of stem cell research, we will try to illustrate how bringing a bioethical viewpoint to the scientific debate can become a healthy exercise in both ethics and science, especially as narratives shift, as was the case in this field due to the introduction of induced pluripotent stem cells, the advent of which is not easily dissociated from the controversies related to embryo research. We should perhaps welcome this trend as promising for the future relationship between ethics and scientific research, providing a stimulus (and not a block) to the ever-evolving scientific discourse.

Near-field photothermal microspectroscopy for adult stem-cell identification and characterization.

PubMed

Grude, Olaug; Hammiche, Azzedine; Pollock, Hubert; Bentley, Adam J; Walsh, Michael J; Martin, Francis L; Fullwood, Nigel J

2007-12-01

The identification of stem cells in adult tissue is a challenging problem in biomedicine. Currently, stem cells are identified by individual epitopes, which are generally tissue specific. The discovery of a stem-cell marker common to other adult tissue types could open avenues in the development of therapeutic stem-cell strategies. We report the use of the novel technique of Fourier transform infrared near-field photothermal microspectroscopy (FTIR-PTMS) for the characterization of stem cells, transit amplifying (TA) cells and terminally differentiated (TD) cells in the corneal epithelium. Principal component analysis (PCA) data demonstrate excellent discrimination of cell type by spectra. PCA in combination with linear discriminant analysis (PCA-LDA) shows that FTIR-PTMS very effectively discriminates between the three cell populations. Statistically significant differences above the 99% confidence level between IR spectra from stem cells and TA cells suggest that nucleic acid conformational changes are an important component of the differences between spectral data from the two cell types. FTIR-PTMS is a new addition to existing spectroscopy methods based on the concept of interfacing a conventional FTIR spectrometer with an atomic force microscope equipped with a near-field thermal sensing probe. FTIR-PTMS spectroscopy currently has spatial resolution that is similar to that of diffraction-limited optical detection FTIR spectroscopy techniques, but as a near-field probing technique has considerable potential for further improvement. Our work also suggests that FTIR-PTMS is potentially more sensitive than synchrotron radiation FTIR spectroscopy for some applications. Microspectroscopy techniques like FTIR-PTMS provide information about the entire molecular composition of cells, in contrast to epitope recognition that only considers the presence or absence of individual molecules. Our results with FTIR-PTMS on corneal stem cells are promising for the potential development of an IR spectral fingerprint for stem cells.

Tested Tools and Techniques for Promoting STEM Programming in Libraries: Fifteen Years of the Lunar and Planetary Institute's Explore Program

NASA Astrophysics Data System (ADS)

LaConte, K.; Shipp, S.; Shupla, C.; Shaner, A.; Buxner, S.; Canipe, M.; Jaksha, A.

2015-11-01

Libraries are evolving to serve the changing needs of their communities—and many now encompass science, technology, engineering, and mathematics (STEM) programming. For 15 years, the Lunar and Planetary Institute (LPI) has partnered with library staff to create over 100 hands-on Earth and space science and engineering activities. In-person and online librarian training has prepared a vibrant network of over 1000 informal educators. Program evaluation has shown that Explore! training increases participants' knowledge, and that participants actively use Explore! materials and feel more prepared to offer science and engineering experiences and more comfortable using related resources. Through training, participants become more committed to providing and advocating for science and engineering programming. Explore! serves as a model for effective product development and training practices for serving library staff, increasingly our partners in the advancement of STEM education. Specific approaches and tools that contributed to the success of Explore! are outlined here for adoption by community STEM experts—including professionals and hobbyists in STEM fields and STEM educators who are seeking to share their passion and experience with others through partnerships with libraries.

Religion and the public ethics of stem-cell research: Attitudes in Europe, Canada and the United States.

PubMed

Allum, Nick; Allansdottir, Agnes; Gaskell, George; Hampel, Jürgen; Jackson, Jonathan; Moldovan, Andreea; Priest, Susanna; Stares, Sally; Stoneman, Paul

2017-01-01

We examine international public opinion towards stem-cell research during the period when the issue was at its most contentious. We draw upon representative sample surveys in Europe and North America, fielded in 2005 and find that the majority of people in Europe, Canada and the United States supported stem-cell research, providing it was tightly regulated, but that there were key differences between the geographical regions in the relative importance of different types of ethical position. In the U.S., moral acceptability was more influential as a driver of support for stem-cell research; in Europe the perceived benefit to society carried more weight; and in Canada the two were almost equally important. We also find that public opinion on stem-cell research was more strongly associated with religious convictions in the U.S. than in Canada and Europe, although many strongly religious citizens in all regions approved of stem-cell research. We conclude that if anything public opinion or 'public ethics' are likely to play an increasingly important role in framing policy and regulatory regimes for sensitive technologies in the future.

Comparison of Marketing Techniques to Enroll Females at a Major Aeronautical University

ERIC Educational Resources Information Center

Cross, David Scott

2012-01-01

Traditionally, industries belonging to the science, technology, engineering, and mathematics (STEM) fields are dominated by males. Recruitment and enrollment of females for STEM fields have become an issue among universities specializing in fields such as aviation and aeronautics. The research problem for this study was that marketing departments…

Why STEM Fields Still Don't Draw More Women

ERIC Educational Resources Information Center

Coger, Robin N.; Cuny, Jan; Klawe, Maria; McGann, Matt; Purcell, Karen D.

2012-01-01

There have been many efforts in recent years to draw more women into STEM fields. While women have made gains, they are still far less likely than men to major in such fields, especially engineering and computer science. Why? This article presents the responses and the thoughts of a group of scholars and experts.

Camp Invention ASL: Inclusive, Relevant, Family-Focused Science

ERIC Educational Resources Information Center

Santini, Joseph

2017-01-01

Among the fields that particularly lack images of diverse participants are those of science, technology, engineering, and math, fields captured under the acronym STEM. Often in STEM fields, images and experiences of deaf and hard of hearing children, children of color, and young women are rare or absent altogether, with the result that these…

Environmental stresses of field growth allow cinnamyl alcohol dehydrogenase-deficient Nicotiana attenuata plants to compensate for their structural deficiencies.

PubMed

Kaur, Harleen; Shaker, Kamel; Heinzel, Nicolas; Ralph, John; Gális, Ivan; Baldwin, Ian T

2012-08-01

The organized lignocellulosic assemblies of cell walls provide the structural integrity required for the large statures of terrestrial plants. Silencing two CINNAMYL ALCOHOL DEHYDROGENASE (CAD) genes in Nicotiana attenuata produced plants (ir-CAD) with thin, red-pigmented stems, low CAD and sinapyl alcohol dehydrogenase activity, low lignin contents, and rubbery, structurally unstable stems when grown in the glasshouse (GH). However, when planted into their native desert habitat, ir-CAD plants produced robust stems that survived wind storms as well as the wild-type plants. Despite efficient silencing of NaCAD transcripts and enzymatic activity, field-grown ir-CAD plants had delayed and restricted spread of red stem pigmentation, a color change reflecting blocked lignification by CAD silencing, and attained wild-type-comparable total lignin contents. The rubbery GH phenotype was largely restored when field-grown ir-CAD plants were protected from wind, herbivore attack, and ultraviolet B exposure and grown in restricted rooting volumes; conversely, it was lost when ir-CAD plants were experimentally exposed to wind, ultraviolet B, and grown in large pots in growth chambers. Transcript and liquid chromatography-electrospray ionization-time-of-flight analysis revealed that these environmental stresses enhanced the accumulation of various phenylpropanoids in stems of field-grown plants; gas chromatography-mass spectrometry and nuclear magnetic resonance analysis revealed that the lignin of field-grown ir-CAD plants had GH-grown comparable levels of sinapaldehyde and syringaldehyde cross-linked into their lignins. Additionally, field-grown ir-CAD plants had short, thick stems with normal xylem element traits, which collectively enabled field-grown ir-CAD plants to compensate for the structural deficiencies associated with CAD silencing. Environmental stresses play an essential role in regulating lignin biosynthesis in lignin-deficient plants.

Environmental Stresses of Field Growth Allow Cinnamyl Alcohol Dehydrogenase-Deficient Nicotiana attenuata Plants to Compensate for their Structural Deficiencies1[C][W][OA

PubMed Central

Kaur, Harleen; Shaker, Kamel; Heinzel, Nicolas; Ralph, John; Gális, Ivan; Baldwin, Ian T.

2012-01-01

The organized lignocellulosic assemblies of cell walls provide the structural integrity required for the large statures of terrestrial plants. Silencing two CINNAMYL ALCOHOL DEHYDROGENASE (CAD) genes in Nicotiana attenuata produced plants (ir-CAD) with thin, red-pigmented stems, low CAD and sinapyl alcohol dehydrogenase activity, low lignin contents, and rubbery, structurally unstable stems when grown in the glasshouse (GH). However, when planted into their native desert habitat, ir-CAD plants produced robust stems that survived wind storms as well as the wild-type plants. Despite efficient silencing of NaCAD transcripts and enzymatic activity, field-grown ir-CAD plants had delayed and restricted spread of red stem pigmentation, a color change reflecting blocked lignification by CAD silencing, and attained wild-type-comparable total lignin contents. The rubbery GH phenotype was largely restored when field-grown ir-CAD plants were protected from wind, herbivore attack, and ultraviolet B exposure and grown in restricted rooting volumes; conversely, it was lost when ir-CAD plants were experimentally exposed to wind, ultraviolet B, and grown in large pots in growth chambers. Transcript and liquid chromatography-electrospray ionization-time-of-flight analysis revealed that these environmental stresses enhanced the accumulation of various phenylpropanoids in stems of field-grown plants; gas chromatography-mass spectrometry and nuclear magnetic resonance analysis revealed that the lignin of field-grown ir-CAD plants had GH-grown comparable levels of sinapaldehyde and syringaldehyde cross-linked into their lignins. Additionally, field-grown ir-CAD plants had short, thick stems with normal xylem element traits, which collectively enabled field-grown ir-CAD plants to compensate for the structural deficiencies associated with CAD silencing. Environmental stresses play an essential role in regulating lignin biosynthesis in lignin-deficient plants. PMID:22645069

Shaking up Pre-Calculus: Incorporating Engineering into K-12 Curricula

ERIC Educational Resources Information Center

Sabo, Chelsea; Burrows, Andrea; Childers, Lois

2014-01-01

Projects highlighting Science, Technology, Engineering, and Mathematics (STEM) education in high schools have promoted student interest in engineering-related fields and enhanced student understanding of mathematics and science concepts. The Science and Technology Enhancement Program (Project STEP), funded by a NSF GK-12 grant at the University of…

76 FR 69242 - Application for New Awards; College Assistance Migrant Program

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-08

... Program: The purpose of CAMP is to provide academic and financial support to help migrant and seasonal... work study, exposure to academic programs and careers in STEM-related fields, and providing support services. These could include services to improve participants' academic skills and knowledge so that they...

Stem cells: a model for screening, discovery and development of drugs.

PubMed

Kitambi, Satish Srinivas; Chandrasekar, Gayathri

2011-01-01

The identification of normal and cancerous stem cells and the recent advances made in isolation and culture of stem cells have rapidly gained attention in the field of drug discovery and regenerative medicine. The prospect of performing screens aimed at proliferation, directed differentiation, and toxicity and efficacy studies using stem cells offers a reliable platform for the drug discovery process. Advances made in the generation of induced pluripotent stem cells from normal or diseased tissue serves as a platform to perform drug screens aimed at developing cell-based therapies against conditions like Parkinson's disease and diabetes. This review discusses the application of stem cells and cancer stem cells in drug screening and their role in complementing, reducing, and replacing animal testing. In addition to this, target identification and major advances in the field of personalized medicine using induced pluripotent cells are also discussed.

Identifying niche-mediated regulatory factors of stem cell phenotypic state: a systems biology approach.

PubMed

Ravichandran, Srikanth; Del Sol, Antonio

2017-02-01

Understanding how the cellular niche controls the stem cell phenotype is often hampered due to the complexity of variegated niche composition, its dynamics, and nonlinear stem cell-niche interactions. Here, we propose a systems biology view that considers stem cell-niche interactions as a many-body problem amenable to simplification by the concept of mean field approximation. This enables approximation of the niche effect on stem cells as a constant field that induces sustained activation/inhibition of specific stem cell signaling pathways in all stem cells within heterogeneous populations exhibiting the same phenotype (niche determinants). This view offers a new basis for the development of single cell-based computational approaches for identifying niche determinants, which has potential applications in regenerative medicine and tissue engineering. © 2017 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

Genetic modification of hematopoietic stem cells: recent advances in the gene therapy of inherited diseases.

PubMed

Bueren, Juan A; Guenechea, Guillermo; Casado, José A; Lamana, María Luisa; Segovia, José C

2003-01-01

Hematopoietic stem cells constitute a rare population of precursor cells with remarkable properties for being used as targets in gene therapy protocols. The last years have been particularly productive both in the fields of gene therapy and stem cell biology. Results from ongoing clinical trials have shown the first unquestionable clinical benefits of immunodeficient patients transplanted with genetically modified autologous stem cells. On the other hand, severe side effects in a few patients treated with gene therapy have also been reported, indicating the usefulness of further improving the vectors currently used in gene therapy clinical trials. In the field of stem cell biology, evidence showing the plastic potential of adult hematopoietic stem cells and data indicating the multipotency of adult mesenchymal precursor cells have been presented. Also, the generation of embryonic stem cells by means of nuclear transfer techniques has appeared as a new methodology with direct implications in gene therapy.

Stem Cell Ophthalmology Treatment Study (SCOTS): bone marrow-derived stem cells in the treatment of Leber's hereditary optic neuropathy

PubMed Central

Weiss, Jeffrey N.; Levy, Steven; Benes, Susan C.

2016-01-01

The Stem Cell Ophthalmology Treatment Study (SCOTS) is currently the largest-scale stem cell ophthalmology trial registered at ClinicalTrials.gov (identifier: NCT01920867). SCOTS utilizes autologous bone marrow-derived stem cells (BMSCs) to treat optic nerve and retinal diseases. Treatment approaches include a combination of retrobulbar, subtenon, intravitreal, intra-optic nerve, subretinal, and intravenous injection of autologous BMSCs according to the nature of the disease, the degree of visual loss, and any risk factors related to the treatments. Patients with Leber's hereditary optic neuropathy had visual acuity gains on the Early Treatment Diabetic Retinopathy Study (ETDRS) of up to 35 letters and Snellen acuity improvements from hand motion to 20/200 and from counting fingers to 20/100. Visual field improvements were noted. Macular and optic nerve head nerve fiber layer typically thickened. No serious complications were seen. The increases in visual acuity obtained in our study were encouraging and suggest that the use of autologous BMSCs as provided in SCOTS for ophthalmologic mitochondrial diseases including Leber's hereditary optic neuropathy may be a viable treatment option. PMID:27904503

Changes in the dielectric properties of a plant stem produced by the application of voltage steps

NASA Astrophysics Data System (ADS)

Hart, F. X.

1983-03-01

Time Domain Dielectric Spectroscopy (TDDS) provides a useful method for monitoring the physiological state of a biological system which may be changing with time. A voltage step is applied to a sample and the Fourier Transform of the resulting current yields the variations of the conductance, capacitance and dielectric loss of the sample with frequency (dielectric spectrum). An important question is whether the application of the voltage step itself can produce changes which obscure those of interest. Long term monitoring of the dielectric properties of plant stems requires the use of needle electrodes with relatively large current densities and field strengths at the electrode-stem interface. Steady currents on the order of those used in TDDS have been observed to modify the distribution of plant growth hormones, to produce wounding at electrode sites, and to cause stem collapse. This paper presents the preliminary results of an investigation into the effects of the application of voltage steps on the observed dielectric spectrum of the stem of the plant Coleus.

Reactive Oxygen Species and Mitochondrial Homeostasis as Regulators of Stem Cell Fate and Function.

PubMed

Tan, Darren Q; Suda, Toshio

2018-07-10

The precise role and impact of reactive oxygen species (ROS) in stem cells, which are essential for lifelong tissue homeostasis and regeneration, remain of significant interest to the field. The long-term regenerative potential of a stem cell compartment is determined by the delicate balance between quiescence, self-renewal, and differentiation, all of which can be influenced by ROS levels. Recent Advances: The past decade has seen a growing appreciation for the importance of ROS and redox homeostasis in various stem cell compartments, particularly those of hematopoietic, neural, and muscle tissues. In recent years, the importance of proteostasis and mitochondria in relation to stem cell biology and redox homeostasis has garnered considerable interest. Here, we explore the reciprocal relationship between ROS and stem cells, with significant emphasis on mitochondria as a core component of redox homeostasis. We discuss how redox signaling, involving cell-fate determining protein kinases and transcription factors, can control stem cell function and fate. We also address the impact of oxidative stress on stem cells, especially oxidative damage of lipids, proteins, and nucleic acids. We further discuss ROS management in stem cells, and present recent evidence supporting the importance of mitochondrial activity and its modulation (via mitochondrial clearance, biogenesis, dynamics, and distribution [i.e., segregation and transfer]) in stem cell redox homeostasis. Therefore, elucidating the intricate links between mitochondria, cellular metabolism, and redox homeostasis is envisioned to be critical for our understanding of ROS in stem cell biology and its therapeutic relevance in regenerative medicine. Antioxid. Redox Signal. 00, 000-000.

Innovations and Challenges in Project-Based STEM Education: Lessons from ITEST

NASA Astrophysics Data System (ADS)

Connors-Kellgren, Alice; Parker, Caroline E.; Blustein, David L.; Barnett, Mike

2016-12-01

For over a decade, the National Science Foundation's Innovative Technology Experiences for Students and Teachers (ITEST) program has funded researchers and educators to build an understanding of best practices, contexts, and processes contributing to K-12 students' motivation and participation in Science, Technology, Engineering, and Mathematics (STEM) activities that lead to STEM career pathways. The outcomes from these projects have contributed significantly to the national body of knowledge about strategies, successes, models, and interventions that support and encourage youth to pursue STEM careers. While the individual projects discussed in this special issue vary by geographic location, institution, populations served, primary focus, and topic, they are unified by ITEST's programmatic intent and goals. This issue offers research-based insights into the knowledge generated by a decade of ITEST-funded work in STEM career development. The articles describe a multitude of approaches to project design, evaluation, and empirical research. Collectively, they contribute to the development of frameworks for STEM education and workforce development that are increasingly relevant for educators, project designers, researchers, and policy makers. The ITEST program has enabled creativity, experimentation, and cultural responsiveness in STEM education and workforce development and broadened participation in STEM initiatives to Native American communities, underresourced urban communities, girls, and populations underrepresented in STEM fields. By approaching research and evaluation with flexibility and resourcefulness, the authors provide empirical evidence for the value of innovative approaches to STEM education that promote STEM interest and career-related outcomes and that build the foundational skills of the scientific and engineering workforce of the future.

Prevention and treatment of relapse after stem cell transplantation by cellular therapies.

PubMed

Falkenburg, Fred; Ruggiero, Eliana; Bonini, Chaira; Porter, David; Miller, Jeff; Malard, Floran; Mohty, Mohamad; Kröger, Nicolaus; Kolb, Hans Jochem

2018-05-24

Despite recent advances in reducing therapy-related mortality after allogeneic stem cell transplantation (alloSCT) relapse remains the major cause of treatment failure and little progress has been achieved in the last decades. At the 3rd International Workshop on Biology, Prevention, and Treatment of Relapse held in Hamburg/Germany in November 2016 international experts presented and discussed recent developments in the field. Here, the potential of cellular therapies including unspecific and specific T cells, genetically modified T cells, CAR-T cells, NK-cells, and second allografting in prevention and treatment of relapse after alloSCT are summarized.

Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation

PubMed Central

Vykoukal, Jody; Vykoukal, Daynene M.; Freyberg, Susanne; Alt, Eckhard U.; Gascoyne, Peter R. C.

2009-01-01

We have applied the microfluidic cell separation method of dielectrophoretic field-flow fractionation (DEP-FFF) to the enrichment of a putative stem cell population from an enzyme-digested adipose tissue derived cell suspension. A DEP-FFF separator device was constructed using a novel microfluidic-microelectronic hybrid flex-circuit fabrication approach that is scaleable and anticipates future low-cost volume manufacturing. We report the separation of a nucleated cell fraction from cell debris and the bulk of the erythrocyte population, with the relatively rare (<2% starting concentration) NG2-positive cell population (pericytes and/or putative progenitor cells) being enriched up to 14-fold. This work demonstrates a potential clinical application for DEP-FFF and further establishes the utility of the method for achieving label-free fractionation of cell subpopulations. PMID:18651083

iSTEM Summer Institute: An Integrated Approach to Teacher Professional Development inSTEM

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anne Seifert; Louis Nadelson

The importance of STEM education to our national prosperity and global competitiveness was recently reinforced by the Obama administration support for Change the Equation. Change the Equation is a multi-entity initiative formed in response to the rapidly increasing demand for STEM related careers and the potential lack of preparation by many Americans to be employed in these positions. To address the issue many are calling for increased emphasis on K-12 STEM education, as early preparation in STEM provides the foundation essential for further learning and competencies (National Research Council, 2007). Achieving and sustaining depth and breadth of K-12 STEM educationmore » is inextricably linked to ongoing professional development of K-12 educators. The need for teacher continuing education in STEM education and the link between teacher effectiveness and student preparation in STEM was the impetus behind our i- STEM professional development summer institute. The i-STEM initiative is a collaborative effort between business, industry, government, K-12, and higher education. Although the organization is working on a number of projects, including policy, research, communication, and collaborations, the i-STEM group has directed significant resources toward K-12 educator professional development opportunities in STEM. Our report focuses on the structure and impact of the intensive four-day i-STEM residential professional development institute which we designed to increase the capacity of grade 4-9 teachers to teach STEM content. We structured the summer institute using the outcome of a survey we conducted of grade 4-9 teachers’ to assess their STEM professional development needs, the extant literature on teacher development, the increasing need for a STEM informed society, and our desire to use evidence based practices to enhance teacher capacity to teach STEM content. We developed this investigation to determine if our summer institute influenced the participating teachers comfort with teaching STEM, efficacy for teaching STEM, content knowledge of STEM, inquiry implementation in STEM, and perceptions of STEM education. These parameters in-part have been gathered previously for specific areas of math or science education, but we are not aware of any study in which these variables have been attended to and assessed in the context of enhancing inservice teacher preparation to teach STEM. Therefore, our project provides a unique contribution to the literature because of our focus on STEM education and our adaptation and use of an array of assessment tools to measure the impact on our participants’ perceptions of teaching STEM, affective perspectives, and knowledge of the related content. Further, we are responding to the position of Putnam and Borko (2000) who contend there has been a dearth of attention paid toward creating teacher professional development experiences consistent with the teacher learning and investigations of the impact of the experience on the participating educators. Before we present our research and results, we discuss the relevant literature establishing the justification for our study. Following the presentation of our study results we discuss the related implications and directions for future research. We conclude with a discussion of study limitations and some closing remarks of our study contributions to the field of teacher professional development in STEM education.« less

Haemopoietic cell renewal in radiation fields

NASA Astrophysics Data System (ADS)

Fliedner, T. M.; Nothdurft, W.; Tibken, B.; Hofer, E.; Weiss, M.; Kindler, H.

1994-10-01

Space flight activities are inevitably associated with a chronic exposure of astronauts to a complex mixture of ionising radiation. Although no acute radiation consequences are to be expected as a rule, the possibility of Solar Particle Events (SPE) associated with relatively high doses of radiation (1 or more Gray) cannot be excluded. It is the responsibility of physicians in charge of the health of astronauts to evaluate before, during and after space flight activities the functional status of haemopoietic cell renewal. Chronic low level exposure of dogs indicate that daily gamma-exposure doses below about 2 cGy are tolerated for several years as far as blood cell concentrations are concerned. However, the stem cell pool may be severely affected. The maintenance of sufficient blood cell counts is possible only through increased cell production to compensate for the radiation inflicted excess cell loss. This behaviour of haemopoietic cell renewal during chronic low level exposure can be simulated by bioengineering models of granulocytopoiesis. It is possible to define a ``turbulence region'' for cell loss rates, below which an prolonged adaptation to increased radiation fields can be expected to be tolerated. On the basis of these experimental results, it is recommended to develop new biological indicators to monitor haemopoietic cell renewal at the level of the stem cell pool using blood stem cells in addition to the determination of cytokine concentrations in the serum (and other novel approaches). To prepare for unexpected haemopoietic effects during prolonged space missions, research should be increased to modify the radiation sensitivity of haemopoietic stem cells (for instance by the application of certain regulatory molecules). In addition, a ``blood stem cell bank'' might be established for the autologous storage of stem cells and for use in space activities keeping them in a radiation protected container.

Multivariate Assessment of Middle School Students' Interest in STEM Career: a Profile from Turkey

NASA Astrophysics Data System (ADS)

Koyunlu Ünlü, Zeynep; Dökme, İlbilge

2018-05-01

According to a report by the Turkish Industry and Business Association, Turkey will need approximately 1 million individuals to be employed in Science Technology Engineering Mathematics (STEM) fields by 2023, and 31% of this requirement will not be met. For continuous economic development, there is a need to integrate STEM into education in Turkey, which brings the need for research in this area. This study, based on a survey model, aimed to determine the level of interest of a sample of Turkish middle school students in STEM careers on the basis of gender, where they lived, grade levels, their end-of-semester grades, and their parents' educational status and levels of income. The research data was collected using the STEM Career Interest Survey (STEM-CIS) and Personal Information Form, which were applied to 851 middle school students (fifth and eighth graders). The collected data was analyzed with SPSS using Mann Whitney U and Kruskal Wallis H tests. It was found that middle school students' interest in STEM careers differed according to sex, where they lived, and grade levels but it did not differ in relation to their parents' educational status and the levels of income of the family. It is believed that the results obtained in this study reflecting the profile in Turkey will guide educational policy makers, curriculum developers, teachers, pre-service teachers, and researchers about STEM education.

Explaining the Gender Gap: Comparing Undergraduate and Graduate/Faculty Beliefs about Talent Required for Success in Academic Fields

NASA Astrophysics Data System (ADS)

Bailey, Kimberlyn; Nanthakumar, Ampalavanar; Preston, Scott; Ilie, Carolina C.

Recent research has proposed that the gender gap in academia is caused by differing perceptions of how much talent is needed to succeed in various fields. It was found that, across the STEM/non-STEM divide, the more that graduate students and faculty see success in their own field as requiring as requiring talent, the fewer women participate in that field. This research examines whether undergraduate students share these attitudes. If these attitudes trickle down to the undergraduate population to influence students to choose different fields of study, then undergraduate beliefs should reflect those of graduate students and faculty. Using a large survey of undergraduates across the country, this study aims to characterize undergraduate attitudes and to determine variables that explain the differences between the attitudes of these two populations. Our findings suggest that the two populations have similar beliefs, but that undergraduate beliefs are strongly influenced by information about the gender ratio in each field and that this strong influence greatly differs between STEM and non-STEM fields. These findings seek to help direct future research to ask the right questions and propose plausible hypotheses about gender the imbalance in academia.

Development and Validation of Scientific Literacy Scale for College Preparedness in STEM with Freshmen from Diverse Institutions

ERIC Educational Resources Information Center

Benjamin, Thomas E.; Marks, Bryant; Demetrikopoulos, Melissa K.; Rose, Jordan; Pollard, Ethen; Thomas, Alicia; Muldrow, Lycurgus L.

2017-01-01

Although a major goal of Science, Technology, Engineering, and Mathematics (STEM) education is to develop scientific literacy, prior efforts at measuring scientific literacy have not attempted to link scientific literacy with success in STEM fields. The current Scientific Literacy Survey for College Preparedness in STEM (SLSCP-STEM) scale was…

Dragons, Ladybugs, and Softballs: Girls' STEM Engagement with Human-Centered Robotics

ERIC Educational Resources Information Center

Gomoll, Andrea; Hmelo-Silver, Cindy E.; Šabanovic, Selma; Francisco, Matthew

2016-01-01

Early experiences in science, technology, engineering, and math (STEM) are important for getting youth interested in STEM fields, particularly for girls. Here, we explore how an after-school robotics club can provide informal STEM experiences that inspire students to engage with STEM in the future. Human-centered robotics, with its emphasis on the…

Supporting the STEM Pipeline: Linking Applied STEM Course-Taking in High School to Declaring a STEM Major in College

ERIC Educational Resources Information Center

Gottfried, Michael A.; Bozick, Robert

2016-01-01

Recently, through the support from the Obama administration, the traditional STEM curricula (science, technology, engineering, and mathematics) in high schools are being updated with integrated, applied STEM courses (e.g., technology and engineering) in order to enhance the "real world" applicability of scientific fields and ultimately…

The Importance of MS PHD'S and SEEDS Mentoring and Professional Development Programs in the Retenion of Underrepresented Minorities in STEM Fields

NASA Astrophysics Data System (ADS)

Strickland, J.; Johnson, A.; Williamson Whitney, V.; Ricciardi, L.

2012-12-01

According to a recent study by the National Academy of Sciences, underrepresented minority (URM) participation in STEM disciplines represents approximately one third of the URM population in the U.S. Thus, the proportion of URM in STEM disciplines would need to triple in order to reflect the demographic makeup in the U.S. Individual programs targeting the recruitment and retention of URM students in STEM have demonstrated that principles of mentoring, community building, networking, and professional skill development are crucial in encouraging URM students to remain in STEM disciplines thereby reducing this disparity in representation. However, to paraphrase an old African proverb, "it takes a village to nurture and develop a URM student entering into the STEM community." Through programs such as the Institute for Broadening Participation's Minorities Striving and Pursuing Higher Degrees of Success (MS PHD'S) Professional Development Program in Earth system science and the Ecological Society of America's Strategies for Ecology Education, Diversity and Sustainability (SEEDS), URM students are successfully identifying and benefitting from meaningful opportunities to develop the professional skills and strategies needed to achieve their academic and career goals. Both programs share a philosophy of professional development, reciprocal mentoring, field trips, internships, employment, research partnerships, collaborations, fellowships, scholarships, grants, and professional meeting travel awards to support URM student retention in STEM. Both programs share a mission to bring more diversity and inclusivity into STEM fields. Both programs share a history of success at facilitating the preparation and advancement of URM students. This success has been documented with the multitude of URM students that have matriculated through the programs and are now actively engaged in the pursuit of advanced degrees in STEM or entering the STEM workforce. Anonymous surveys from participants affirms that these programs provided an excellent environment for advancing interest in, and knowledge of STEM, and for influencing academic career goals for participants. These programs are models and reflect the importance of providing diversity, mentoring and professional development programs to broaden the participation and retention of URM students in STEM fields.

Pathways into STEM among low-income, urban immigrant emergent bilingual/multilingual young adults: Opportunity, access, and persistence

NASA Astrophysics Data System (ADS)

Heyman, Jeremy Benjamin

This project builds upon the author's multi-year critical ethnographic study of urban immigrant students and their trajectories into STEM (science, technology, engineering, or mathematics) from high school through their transition to college. At its core, this study investigates the paths of over three dozen newcomer immigrant English language learner students in high-poverty urban neighborhoods who are not generally considered "legitimate contenders" for Bachelor's degrees in STEM fields on the basis of such characteristics as test scores, high school and prior preparation, and age. The students are followed through their high school experiences, their transition to college, and through their current progress in college, with explicit attention paid to key mediating experiences and relationships in and especially outside of the classroom that were associated with their toward persistence and success. Thick description and analysis of the students and their experiences, among those who persisted as well as the minority who switched out of STEM majors, helps to demonstrate a proof-of-concept of these students' ability to succeed while painting a comprehensive picture of their march forward to degrees in STEM fields against a backdrop of economic, linguistic, and other barriers to entry and success. Using a framework of social and capital and resilience theories, this work has uncovered a number of themes and factors that will help educators to better understand the evolution of these traditionally marginalized students' STEM-related interests, skills, and career plans. The findings center around students' exposure to research internships and other STEM enrichment and outreach experiences, long-term mentoring and other key relationships, and integration of STEM and college access efforts in setting them up for a successful transition to college, as well as an emphasis on the importance of students' calling upon their own resilience and other strengths and prior experiences. The results provide novel insights and recommendations for improving access and persistence in STEM among students in areas of concentrated poverty who are also struggling with mastering a new language and a host of other challenges.

Globalization of Stem Cell Science: An Examination of Current and Past Collaborative Research Networks

PubMed Central

Luo, Jingyuan; Matthews, Kirstin R. W.

2013-01-01

Science and engineering research has becoming an increasingly international phenomenon. Traditional bibliometric studies have not captured the evolution of collaborative partnerships between countries, particularly in emerging technologies such as stem cell science, in which an immense amount of investment has been made in the past decade. Analyzing over 2,800 articles from the top journals that include stem cell research in their publications, this study demonstrates the globalization of stem cell science. From 2000 to 2010, international collaborations increased from 20.9% to 36% of all stem cell publications analyzed. The United States remains the most prolific and the most dominant country in the field in terms of publications in high impact journals. But Asian countries, particularly China are steadily gaining ground. Exhibiting the largest relative growth, the percent of Chinese-authored stem cell papers grew more than ten-fold, while the percent of Chinese-authored international papers increased over seven times from 2000 to 2010. And while the percent of total stem cell publications exhibited modest growth for European countries, the percent of international publications increased more substantially, particularly in the United Kingdom. Overall, the data indicated that traditional networks of collaboration extant in 2000 still predominate in stem cell science. Although more nations are becoming involved in international collaborations and undertaking stem cell research, many of these efforts, with the exception of those in certain Asian countries, have yet to translate into publications in high impact journals. PMID:24069210

Globalization of stem cell science: an examination of current and past collaborative research networks.

PubMed

Luo, Jingyuan; Matthews, Kirstin R W

2013-01-01

Science and engineering research has becoming an increasingly international phenomenon. Traditional bibliometric studies have not captured the evolution of collaborative partnerships between countries, particularly in emerging technologies such as stem cell science, in which an immense amount of investment has been made in the past decade. Analyzing over 2,800 articles from the top journals that include stem cell research in their publications, this study demonstrates the globalization of stem cell science. From 2000 to 2010, international collaborations increased from 20.9% to 36% of all stem cell publications analyzed. The United States remains the most prolific and the most dominant country in the field in terms of publications in high impact journals. But Asian countries, particularly China are steadily gaining ground. Exhibiting the largest relative growth, the percent of Chinese-authored stem cell papers grew more than ten-fold, while the percent of Chinese-authored international papers increased over seven times from 2000 to 2010. And while the percent of total stem cell publications exhibited modest growth for European countries, the percent of international publications increased more substantially, particularly in the United Kingdom. Overall, the data indicated that traditional networks of collaboration extant in 2000 still predominate in stem cell science. Although more nations are becoming involved in international collaborations and undertaking stem cell research, many of these efforts, with the exception of those in certain Asian countries, have yet to translate into publications in high impact journals.

Summer Center for Climate, Energy, and Environmental Decision Making (SUCCEED)

NASA Astrophysics Data System (ADS)

Klima, K.; Hoss, F.; Welle, P.; Larkin, S.

2013-12-01

Science, Technology, and Math (STEM) fields are responsible for more than half of our sustained economic expansion, and over the past 25 years the science and engineering workforce has remained at over 5% of all U.S. jobs. However, America lags behind other nations when it comes to STEM education; globally, American students rank 23th in math and 31st in science. While our youngest students show an interest in STEM subjects, roughly 40% of college students planning to major in STEM switch to other subjects. Women and minorities, 50% and 43% of school-age children, are disproportionally underrepresented in STEM fields (25% and 15%, respectively). Studies show that improved teacher curriculum combined with annual student-centered learning summer programs can promote and sustain student interest in STEM fields. Many STEM fields appear superficially simple, and yet can be truly complex and controversial topics. Carnegie Mellon University's Center for Climate and Energy Decision Making focuses on two such STEM fields: climate and energy. In 2011, we created SUCCEED: the Summer Center for Climate, Energy, and Environmental Decision Making. SUCCEED consisted of two pilot programs: a 2-day workshop for K-12 teacher professional development and a free 5-day summer school targeted at an age gap in the university's outreach, students entering 10th grade. In addition to teaching lessons climate, energy, and environment, the program aimed to highlight different STEM careers so students could better understand the breadth of choices available. SUCCEED, repeated in 2012, was wildly successful. A pre/post test demonstrated a significant increase in understanding of STEM topics. Furthermore, SUCCEED raised excitement for STEM; teachers were enthusiastic about accurate student-centered learning plans and students wanted to know more. To grow these efforts, an additional component has been added to the SUCCEED 2013 effort: online publicly available curricula. Using the curricula form 2011-2013 as base material, we have been developing online publically available Pennsylvania lesson plans meeting Next Generation Science Standards or Common Core Math Standards. The teacher curricula database will greatly increase our ability to correct misconceptions and fill gaps in lessons taught to thousands of students. This talk will share more about the SUCCEED program and the teacher curricula database efforts.

Differences in students' perceived classroom experiences by instructor gender, student gender, and persistence in STEM courses

NASA Astrophysics Data System (ADS)

Fowlkes, Carol

Science, technology, engineering, and mathematics (STEM) fields are growing and have lucrative job opportunities for college graduates. However, the number of students in STEM majors and the number of those who persist in those majors is declining; there is also a growing gender gap in STEM graduates. This study investigated three perceived classroom experiences in STEM courses and the nature of differences in these experiences by student gender, instructor gender, and by those who persisted or did not persist in STEM majors. A factorial MANOVA was the statistical method by which the differences were explored. The statistical analysis revealed non-significant mean differences in three-way interaction, all two-way interactions, and all main effects. There were not gendered differences in students' perceptions of the opportunities for hands-on learning, the instructor cares about students' success, and the instructor encourages students' contributions. Further research is proposed to continue examination of this topic with a larger data set that is consistent with the literature regarding the population of STEM students and the number of STEM persisters, and the male-gendered nature of STEM fields.

The immune boundaries for stem cell based therapies: problems and prospective solutions

PubMed Central

Abdelkrim, Hmadcha; Juan, Domínguez-Bendala; Jane, Wakeman; Mohamed, Arredouani; Bernat, Soria

2009-01-01

Stem cells have fascinated the scientific and clinical communities for over a century. Despite the controversy that surrounds this field, it is clear that stem cells have the potential to revolutionize medicine. However, a number of significant hurdles still stand in the way of the realization of this potential. Chiefly among these are safety concerns, differentiation efficiency and overcoming immune rejection. Here we review current progress made in this field to optimize the safe use of stem cells with particular emphasis on prospective interventions to deal with challenges generated by immune rejection. PMID:19583810

Students who demonstrate strong talent and interest in STEM are initially attracted to STEM through extracurricular experiences.

PubMed

VanMeter-Adams, Amy; Frankenfeld, Cara L; Bases, Jessica; Espina, Virginia; Liotta, Lance A

2014-01-01

What early experiences attract students to pursue an education and career in science, technology, engineering, and mathematics (STEM)? Does hands-on research influence them to persevere and complete a major course of academic study in STEM? We evaluated survey responses from 149 high school and undergraduate students who gained hands-on research experience in the 2007-2013 Aspiring Scientists Summer Internship Programs (ASSIP) at George Mason University. Participants demonstrated their strong interest in STEM by volunteering to participate in ASSIP and completing 300 h of summer research. The survey queried extracurricular experiences, classroom factors, and hands-on projects that first cultivated students' interest in the STEM fields, and separately evaluated experiences that sustained their interest in pursuing a STEM degree. The majority of students (65.5%, p < 0.0001) reported extracurricular encounters, such as the influence of a relative or family member and childhood experiences, as the most significant factors that initially ignited their interest in STEM, while hands-on lab work was stated as sustaining their interest in STEM (92.6%). Based on these findings collected from a cohort of students who demonstrated a strong talent and interest in STEM, community-based programs that create awareness about STEM for both children and their family members may be key components for igniting long-term academic interest in STEM. © 2014 A. VanMeter-Adams et al. CBE—Life Sciences Education © 2014 The American Society for Cell Biology. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

AMS Climate Studies Diversity Project and its Relationship to a Concentration in STEM Discipline at Alabama A&M University

NASA Astrophysics Data System (ADS)

Oluwoye, J.

2017-12-01

The American Meteorological Society (AMS) reported that our nation faces a serious challenge in attracting young people to science and science-related careers (including teaching). This is particularly true for members of groups underrepresented in science, technology, engineering, and mathematics (STEM) and is especially acute in the small number of minority college students majoring in the geosciences. The purpose of this paper is to report on how the author engages Alabama A&M University (AAMU) students in STEM transportation science. Specifically, the objective is to develop a conceptual framework of engaging minority students in transportation concentration in the department of community and regional planning. The students were involved in writing a research paper on direct and indirect climate change impacts on transportation and also involved in classroom discussions during a wk14 module on overview of transportation suitability: climate change and environment. The paper concludes with minority needs to gain access to STEM and participation of minority students in field and site analysis.

Evaluation of crystallographic strain, rotation and defects in functional oxides by the moiré effect in scanning transmission electron microscopy.

PubMed

Naden, A B; O'Shea, K J; MacLaren, D A

2018-04-20

Moiré patterns in scanning transmission electron microscopy (STEM) images of epitaxial perovskite oxides are used to assess strain and defect densities over fields of view extending over several hundred nanometers. The patterns arise from the geometric overlap of the rastered STEM electron beam and the samples' crystal periodicities and we explore the emergence and application of these moiré fringes for rapid strain analysis. Using the epitaxial functional oxide perovskites BiFeO 3 and Pr 1-x Ca x MnO 3 , we discuss the impact of large degrees of strain on the quantification of STEM moiré patterns, identify defects in the fringe patterns and quantify strain and lattice rotation. Such a wide-area analysis of crystallographic strain and defects is crucial for developing structure-function relations of functional oxides and we find the STEM moiré technique to be an attractive means of structural assessment that can be readily applied to low dose studies of damage sensitive crystalline materials.

Evaluation of crystallographic strain, rotation and defects in functional oxides by the moiré effect in scanning transmission electron microscopy

NASA Astrophysics Data System (ADS)

Naden, A. B.; O'Shea, K. J.; MacLaren, D. A.

2018-04-01

Moiré patterns in scanning transmission electron microscopy (STEM) images of epitaxial perovskite oxides are used to assess strain and defect densities over fields of view extending over several hundred nanometers. The patterns arise from the geometric overlap of the rastered STEM electron beam and the samples’ crystal periodicities and we explore the emergence and application of these moiré fringes for rapid strain analysis. Using the epitaxial functional oxide perovskites BiFeO3 and Pr1-x Ca x MnO3, we discuss the impact of large degrees of strain on the quantification of STEM moiré patterns, identify defects in the fringe patterns and quantify strain and lattice rotation. Such a wide-area analysis of crystallographic strain and defects is crucial for developing structure-function relations of functional oxides and we find the STEM moiré technique to be an attractive means of structural assessment that can be readily applied to low dose studies of damage sensitive crystalline materials.

Predictors of Latina/o Community College Student Vocational Choice of STEM Fields: Testing of the STEM-Vocational Choice Model

ERIC Educational Resources Information Center

Johnson, Joel D.

2013-01-01

This study confirmed appropriate measurement model fit for a theoretical model, the STEM vocational choice (STEM-VC) model. This model identifies exogenous factors that successfully predicted, at a statistically significant level, a student's vocational choice decision to pursue a STEM degree at transfer. The student population examined for this…

Women in the Academy: Female Leadership in STEM Education and the Evolution of a Mentoring Web

ERIC Educational Resources Information Center

Gorman, Susan T.; Durmowicz, Meredith C.; Roskes, Ellen M.; Slattery, Susan P.

2010-01-01

Women continue to be underrepresented in science, technology, engineering, and mathematics (STEM) fields and in STEM leadership positions. According to the most recent data available from the National Science Foundation, in academia only 31% of full-time STEM faculty and 27% of STEM deans and department heads are women. By comparison at Stevenson…

Development of bioengineering system for stem cell proliferation

NASA Astrophysics Data System (ADS)

Park, H. S.; Shah, R.; Shah, C.

2016-08-01

From last decades, intensive research in the field of stem cells proliferation had been promoted due to the unique property of stem cells to self-renew themselves into multiples and has potential to replicate into an organ or tissues and so it's highly demanding though challenging. Bioreactor, a mechanical device, works as a womb for stem cell proliferation by providing nutritious environment for the proper growth of stem cells. Various factors affecting stem cells growth are the bioreactor mechanism, feeding of continuous nutrients, healthy environment, etc., but it always remains a challenge for controlling biological parameters. The present paper unveils the design of mechanical device commonly known as bioreactor in tissues engineering and biotech field, use for proliferation of stem cells and imparts the proper growing condition for stem cells. This high functional bioreactor provides automation mixing of cell culture and stem cells. This design operates in conjunction with mechanism of reciprocating motion. Compare to commercial bioreactors, this proposed design is more convenient, easy to operate and less maintenance is required as bioreactor culture bag is made of polyethylene which is single use purpose. Development of this bioengineering system will be beneficial for better growth and expansion of stem cell

Promoting Diversity in STEM through Active Recruiting and Mentoring: The Pre-Major in Astronomy Program (Pre-MAP) at the University of Washington

NASA Astrophysics Data System (ADS)

Schwieterman, Edward; Binder, Breanna; Tremmel, Michael; Garofali, Kristen; Agol, Eric; Meadows, Victoria

2015-11-01

The Pre-Major in Astronomy Program (Pre-MAP) is a research and mentoring program for underclassmen and transfer students offered by the University of Washington Astronomy Department since 2005. The primary goal of Pre-MAP is to recruit and retain students from groups traditionally underrepresented in science, technology, engineering, and mathematics (STEM) through early exposure to research. The Pre-MAP seminar is the core component of the program and offers instruction in computing skills, data manipulation, science writing, statistical analysis, and scientific speaking and presentation skills. Students choose research projects proposed by faculty, post-docs and graduate students in areas related to astrophysics, planetary science, and astrobiology. Pre-MAP has been successful in retaining underrepresented students in STEM fields relative to the broader UW population, and we've found these students are more likely to graduate and excel academically than their peers. As of spring 2015, more than one hundred students have taken the Pre-MAP seminar, and both internal and external evaluations have shown that all groups of participating students report an increased interest in astronomy and science careers at the end of the seminar. Several former Pre-MAP students have obtained or are pursuing doctoral and master’s degrees in STEM fields; many more work at NASA centers, teaching colleges, or as engineers or data analysts. Pre- MAP student research has produced dozens of publications in peer-reviewed research journals. This talk will provide an overview of the program: the structure of the seminar, examples of projects completed by students, cohort-building activities outside the seminar, funding sources, recruitment strategies, and the aggregate demographic and achievement data of our students. It is our hope that similar programs may be adopted successfully at other institutions.

Quality of evidence revealing subtle gender biases in science is in the eye of the beholder

PubMed Central

Handley, Ian M.; Brown, Elizabeth R.; Moss-Racusin, Corinne A.; Smith, Jessi L.

2015-01-01

Scientists are trained to evaluate and interpret evidence without bias or subjectivity. Thus, growing evidence revealing a gender bias against women—or favoring men—within science, technology, engineering, and mathematics (STEM) settings is provocative and raises questions about the extent to which gender bias may contribute to women’s underrepresentation within STEM fields. To the extent that research illustrating gender bias in STEM is viewed as convincing, the culture of science can begin to address the bias. However, are men and women equally receptive to this type of experimental evidence? This question was tested with three randomized, double-blind experiments—two involving samples from the general public (n = 205 and 303, respectively) and one involving a sample of university STEM and non-STEM faculty (n = 205). In all experiments, participants read an actual journal abstract reporting gender bias in a STEM context (or an altered abstract reporting no gender bias in experiment 3) and evaluated the overall quality of the research. Results across experiments showed that men evaluate the gender-bias research less favorably than women, and, of concern, this gender difference was especially prominent among STEM faculty (experiment 2). These results suggest a relative reluctance among men, especially faculty men within STEM, to accept evidence of gender biases in STEM. This finding is problematic because broadening the participation of underrepresented people in STEM, including women, necessarily requires a widespread willingness (particularly by those in the majority) to acknowledge that bias exists before transformation is possible. PMID:26460001

The Potential Role of Science, Technology, Engineering, and Math Programs in Reducing Teen Dating Violence and Intimate Partner Violence.

PubMed

D'Inverno, Ashley Schappell; Kearns, Megan C; Reidy, Dennis E

2016-12-01

Science, technology, engineering, and math (STEM) are growing fields that provide job stability, financial security, and health prosperity for professionals in these fields. Unfortunately, females are underrepresented in STEM, which is potentially both a consequence and precipitant of gender inequity in the United States. In addition to the financial and health benefits, increasing the number of girls and women in STEM fields may also indirectly prevent and/or reduce teen dating violence and intimate partner violence by: (1) increasing women's financial independence, thereby reducing dependence on potentially abusive partners; (2) decreasing household poverty and financial stress, which may lead to reductions in relationship discord; and (3) increasing attitudes and beliefs about women as equals, thereby increasing gender equity. In this commentary, we discuss the potential role of primary and secondary school STEM programs in reducing violence against women. We review the literature on existing evaluations of STEM programs for educational outcomes, discuss the limitations of these evaluations, and offer suggestions for future research.

Abundance and Spatial Dispersion of Rice Stem Borer Species in Kahama, Tanzania

PubMed Central

Leonard, Alfonce; Rwegasira, Gration M.

2015-01-01

Species diversity, abundance, and dispersion of rice stem borers in framer’s fields were studied in four major rice growing areas of Kahama District. Stem borer larvae were extracted from the damaged tillers in 16 quadrants established in each field. Adult Moths were trapped by light traps and collected in vials for identification. Results indicated the presence of Chilo partellus, Maliarpha separatella, and Sesamia calamistis in all study areas. The most abundant species was C. partellus (48.6%) followed by M. separatella (35.4%) and S. calamistis was least abundant (16.1%). Stem borers dispersion was aggregated along the edges of rice fields in three locations (wards) namely: Bulige, Chela, and Ngaya. The dispersion in the fourth ward, Kashishi was uniform as established from two of the three dispersion indices tested. Further studies would be required to establish the available alternative hosts, the extent of economic losses and the distribution of rice stem borers in the rest of the Lake zone of Tanzania. PMID:26411785

Attracting STEM talent: do STEM students prefer traditional or work/life-interaction labs?

PubMed

DeFraine, William C; Williams, Wendy M; Ceci, Stephen J

2014-01-01

The demand for employees trained in science, technology, engineering, and mathematics (STEM) fields continues to increase, yet the number of Millennial students pursuing STEM is not keeping pace. We evaluated whether this shortfall is associated with Millennials' preference for flexibility and work/life-interaction in their careers-a preference that may be inconsistent with the traditional idea of a science career endorsed by many lab directors. Two contrasting approaches to running STEM labs and training students were explored, and we created a lab recruitment video depicting each. The work-focused video emphasized the traditional notions of a science lab, characterized by long work hours and a focus on individual achievement and conducting research above all else. In contrast, the work/life-interaction-focused video emphasized a more progressive view - lack of demarcation between work and non-work lives, flexible hours, and group achievement. In Study 1, 40 professors rated the videos, and the results confirmed that the two lab types reflected meaningful real-world differences in training approaches. In Study 2, we recruited 53 current and prospective graduate students in STEM fields who displayed high math-identification and a commitment to science careers. In a between-subjects design, they watched one of the two lab-recruitment videos, and then reported their anticipated sense of belonging to and desire to participate in the lab depicted in the video. Very large effects were observed on both primary measures: Participants who watched the work/life-interaction-focused video reported a greater sense of belonging to (d = 1.49) and desire to participate in (d = 1.33) the lab, relative to participants who watched the work-focused video. These results suggest Millennials possess a strong desire for work/life-interaction, which runs counter to the traditional lab-training model endorsed by many lab directors. We discuss implications of these findings for STEM recruitment.

Attracting STEM Talent: Do STEM Students Prefer Traditional or Work/Life-Interaction Labs?

PubMed Central

DeFraine, William C.; Williams, Wendy M.; Ceci, Stephen J.

2014-01-01

The demand for employees trained in science, technology, engineering, and mathematics (STEM) fields continues to increase, yet the number of Millennial students pursuing STEM is not keeping pace. We evaluated whether this shortfall is associated with Millennials' preference for flexibility and work/life-interaction in their careers-a preference that may be inconsistent with the traditional idea of a science career endorsed by many lab directors. Two contrasting approaches to running STEM labs and training students were explored, and we created a lab recruitment video depicting each. The work-focused video emphasized the traditional notions of a science lab, characterized by long work hours and a focus on individual achievement and conducting research above all else. In contrast, the work/life-interaction-focused video emphasized a more progressive view – lack of demarcation between work and non-work lives, flexible hours, and group achievement. In Study 1, 40 professors rated the videos, and the results confirmed that the two lab types reflected meaningful real-world differences in training approaches. In Study 2, we recruited 53 current and prospective graduate students in STEM fields who displayed high math-identification and a commitment to science careers. In a between-subjects design, they watched one of the two lab-recruitment videos, and then reported their anticipated sense of belonging to and desire to participate in the lab depicted in the video. Very large effects were observed on both primary measures: Participants who watched the work/life-interaction-focused video reported a greater sense of belonging to (d = 1.49) and desire to participate in (d = 1.33) the lab, relative to participants who watched the work-focused video. These results suggest Millennials possess a strong desire for work/life-interaction, which runs counter to the traditional lab-training model endorsed by many lab directors. We discuss implications of these findings for STEM recruitment. PMID:24587044

Signaling mechanisms regulating adult neural stem cells and neurogenesis

PubMed Central

Faigle, Roland; Song, Hongjun

2012-01-01

Background Adult neurogenesis occurs throughout life in discrete regions of the mammalian brain and is tightly regulated via both extrinsic environmental influences and intrinsic genetic factors. In recent years, several crucial signaling pathways have been identified in regulating self-renewal, proliferation, and differentiation of neural stem cells, as well as migration and functional integration of developing neurons in the adult brain. Scope of review Here we review our current understanding of signaling mechanisms, including Wnt, notch, sonic hedgehog, growth and neurotrophic factors, bone morphogenetic proteins, neurotransmitters, transcription factors, and epigenetic modulators, and crosstalk between these signaling pathways in the regulation of adult neurogenesis. We also highlight emerging principles in the vastly growing field of adult neural stem cell biology and neural plasticity. Major conclusions Recent methodological advances have enabled the field to identify signaling mechanisms that fine-tune and coordinate neurogenesis in the adult brain, leading to a better characterization of both cell-intrinsic and environmental cues defining the neurogenic niche. Significant questions related to niche cell identity and underlying regulatory mechanisms remain to be fully addressed and will be the focus of future studies. General significance A full understanding of the role and function of individual signaling pathways in regulating neural stem cells and generation and integration of newborn neurons in the adult brain may lead to targeted new therapies for neurological diseases in humans. PMID:22982587

Major Gene for Field Stem Rust Resistance Co-Locates with Resistance Gene Sr12 in ‘Thatcher’ Wheat

PubMed Central

Hiebert, Colin W.; Kolmer, James A.; McCartney, Curt A.; Briggs, Jordan; Fetch, Tom; Bariana, Harbans; Choulet, Frederic; Rouse, Matthew N.; Spielmeyer, Wolfgang

2016-01-01

Stem rust, caused by Puccinia graminis (Pgt), is a damaging disease of wheat that can be controlled by utilizing effective stem rust resistance genes. ‘Thatcher’ wheat carries complex resistance to stem rust that is enhanced in the presence of the resistance gene Lr34. The purpose of this study was to examine APR in ‘Thatcher’ and look for genetic interactions with Lr34. A RIL population was tested for stem rust resistance in field nurseries in Canada, USA, and Kenya. BSA was used to find SNP markers associated with reduced stem rust severity. A major QTL was identified on chromosome 3BL near the centromere in all environments. Seedling testing showed that Sr12 mapped to the same region as the QTL for APR. The SNP markers were physically mapped and the region carrying the resistance was searched for sequences with homology to members of the NB-LRR resistance gene family. SNP marker from one NB-LRR-like sequence, NB-LRR3 co-segregated with Sr12. Two additional populations, including one that lacked Lr34, were tested in field nurseries. NB-LRR3 mapped near the maximum LOD for reduction in stem rust severity in both populations. Lines from a population that segregated for Sr12 and Lr34 were tested for seedling Pgt biomass and infection type, as well as APR to field stem rust which showed an interaction between the genes. We concluded that Sr12, or a gene closely linked to Sr12, was responsible for ‘Thatcher’-derived APR in several environments and this resistance was enhanced in the presence of Lr34. PMID:27309724

Major Gene for Field Stem Rust Resistance Co-Locates with Resistance Gene Sr12 in 'Thatcher' Wheat.

PubMed

Hiebert, Colin W; Kolmer, James A; McCartney, Curt A; Briggs, Jordan; Fetch, Tom; Bariana, Harbans; Choulet, Frederic; Rouse, Matthew N; Spielmeyer, Wolfgang

2016-01-01

Stem rust, caused by Puccinia graminis (Pgt), is a damaging disease of wheat that can be controlled by utilizing effective stem rust resistance genes. 'Thatcher' wheat carries complex resistance to stem rust that is enhanced in the presence of the resistance gene Lr34. The purpose of this study was to examine APR in 'Thatcher' and look for genetic interactions with Lr34. A RIL population was tested for stem rust resistance in field nurseries in Canada, USA, and Kenya. BSA was used to find SNP markers associated with reduced stem rust severity. A major QTL was identified on chromosome 3BL near the centromere in all environments. Seedling testing showed that Sr12 mapped to the same region as the QTL for APR. The SNP markers were physically mapped and the region carrying the resistance was searched for sequences with homology to members of the NB-LRR resistance gene family. SNP marker from one NB-LRR-like sequence, NB-LRR3 co-segregated with Sr12. Two additional populations, including one that lacked Lr34, were tested in field nurseries. NB-LRR3 mapped near the maximum LOD for reduction in stem rust severity in both populations. Lines from a population that segregated for Sr12 and Lr34 were tested for seedling Pgt biomass and infection type, as well as APR to field stem rust which showed an interaction between the genes. We concluded that Sr12, or a gene closely linked to Sr12, was responsible for 'Thatcher'-derived APR in several environments and this resistance was enhanced in the presence of Lr34.

Single atom visibility in STEM optical depth sectioning

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ishikawa, Ryo; Pennycook, Stephen J.; Lupini, Andrew R.

The continuing development of aberration correctors for the scanning transmission electron microscope (STEM) offers the possibility of locating single atoms in crystals in 3D via optical depth sectioning. The main factors that determine the feasibility of such an approach are visibility and dose requirements. In this paper, we show how Poisson's statistics can be quantitatively incorporated into STEM image simulations and demonstrate that the 3D location of single cerium atoms in wurtzite-type aluminum nitride is indeed feasible under large-angle illumination conditions with a relatively low dose. We also show that chromatic aberration does not presently represent a limitation provided amore » cold field emission source is used. Finally, these results suggest efforts into improved aberration corrector designs for larger illumination angles that offer significant potential for 3D structure determination of materials.« less

Single atom visibility in STEM optical depth sectioning

DOE PAGES

Ishikawa, Ryo; Pennycook, Stephen J.; Lupini, Andrew R.; ...

2016-10-19

The continuing development of aberration correctors for the scanning transmission electron microscope (STEM) offers the possibility of locating single atoms in crystals in 3D via optical depth sectioning. The main factors that determine the feasibility of such an approach are visibility and dose requirements. In this paper, we show how Poisson's statistics can be quantitatively incorporated into STEM image simulations and demonstrate that the 3D location of single cerium atoms in wurtzite-type aluminum nitride is indeed feasible under large-angle illumination conditions with a relatively low dose. We also show that chromatic aberration does not presently represent a limitation provided amore » cold field emission source is used. Finally, these results suggest efforts into improved aberration corrector designs for larger illumination angles that offer significant potential for 3D structure determination of materials.« less

Changing the Image of CTE

ERIC Educational Resources Information Center

Kidwai, Sabrina

2011-01-01

Times have changed. Global economic competition is increasing and the need to develop a workforce with advanced skills is critical. The push to find sources of sustainable energy, the growing demands of the health care field and that of science, technology, engineering, and math (STEM)-related sectors are all driving the high-demand jobs in…

Interior Design Supports Art Education: A Case Study

ERIC Educational Resources Information Center

Clemons, Stephanie A.

2006-01-01

Interior design, as a field of study, is a rapidly growing area of interest--particularly for teenagers in the United States. Part of this interest stems from the proliferation of design-related reality shows available through television media. Some art educators and curriculum specialists in the nation perceive the study of interior spaces as a…

The Effects of Motivation on Student Performance on Science Assessments

ERIC Educational Resources Information Center

Glenn, Tina Heard

2013-01-01

Academic achievement of public school students in the United States has significantly fallen behind other countries. Students' lack of knowledge of, or interest in, basic science and math has led to fewer graduates of science, technology, engineering, and math-related fields (STEM), a factor that may affect their career success and will certainly…

Transforming a Traditional Inquiry-Based Science Unit into a STEM Unit for Elementary Pre-service Teachers: A View from the Trenches

NASA Astrophysics Data System (ADS)

Schmidt, Matthew; Fulton, Lori

2016-04-01

The need to prepare students with twenty-first-century skills through STEM-related teaching is strong, especially at the elementary level. However, most teacher education preparation programs do not focus on STEM education. In an attempt to provide an exemplary model of a STEM unit, we used a rapid prototyping approach to transform an inquiry-based unit on moon phases into one that integrated technology in a meaningful manner to develop technological literacy and scientific concepts for pre-service teachers (PSTs). Using qualitative case study methodology, we describe lessons learned related to the development and implementation of a STEM unit in an undergraduate elementary methods course, focusing on the impact the inquiry model had on PSTs' perceptions of inquiry-based science instruction and how the integration of technology impacted their learning experience. Using field notes and survey data, we uncovered three overarching themes. First, we found that PSTs held absolutist beliefs and had a need for instruction on inquiry-based learning and teaching. Second, we determined that explicit examples of effective and ineffective technology use are needed to help PSTs develop an understanding of meaningful technology integration. Finally, the rapid prototyping approach resulted in a successful modification of the unit, but caused the usability of our digital instructional materials to suffer. Our findings suggest that while inquiry-based STEM units can be implemented in existing programs, creating and testing these prototypes requires significant effort to meet PSTs' learning needs, and that iterating designs is essential to successful implementation.

Persistence of Undergraduate Women in STEM Fields

ERIC Educational Resources Information Center

Pedone, Maggie Helene

2016-01-01

The underrepresentation of women in science, technology, engineering, and mathematics (STEM) is a complex problem that continues to persist at the postsecondary level, particularly in computer science and engineering fields. This dissertation explored the pre-college and college level factors that influenced undergraduate women's persistence in…

From community college to 4-year institutions: Latinas' successful completion of STEM baccalaureate degrees

NASA Astrophysics Data System (ADS)

Zamudio, Rocio

The purpose of this qualitative study was to explore the narrative of Latina graduates who successfully navigated through community college and 4-year institutions in the STEM fields. Rather than focus on what these students lack as much of the current research does, the study explored what assets these students bring that supports their success in STEM fields. Utilizing an ethnographic interview approach, participants who attained STEM baccalaureates in California were interviewed. Qualitative findings revealed various experiences, attitudes, and cultural influences that led to successful completion of a STEM degree. First, the study found that successful community college Latina STEM graduates exhibit grit, are resilient, determined, and have positive attitudes about their underrepresentation in STEM. Second, participants sought after peer, faculty, and staff relationships that helped them be successful. Lastly, participants had the support of their families and reported a high level of connectedness to their culture. Implications, recommendations for practice, and directions for future research are discussed.

Pathways in STEM: Factors Affecting the Retention and Attrition of Talented Men and Women from the STEM Pipeline

ERIC Educational Resources Information Center

Heilbronner, Nancy N.

2009-01-01

Many men and women who are talented in science, technology, engineering, and/or mathematics (STEM) choose not to pursue undergraduate majors or careers in these fields. To develop talents in STEM, educators must understand the factors that contribute to an individual's retention in STEM domains, as well as the factors that act as barriers to…

The Science Identity and Entering a Science Occupation*

PubMed Central

Stets, Jan E.; Brenner, Philip S.; Burke, Peter J.; Serpe, Richard T.

2016-01-01

The initiative to increase the number of students in STEM disciplines and train them for a science-related job is a current national focus. Using longitudinal panel data from a national study that followed underrepresented college students in STEM fields, we investigate the neglected role that social psychological processes play in influencing science activity among the young. We study the impact of identity processes related to being a science student on entering a science occupation. More broadly, we examine whether an identity formulated in one institutional setting (education) has effects that persist to another institutional setting (the economy). We find that the science identity positively impacts the likelihood of entering a science occupation. It also serves as a mediator for other factors that are related to educational success. This provides insight into how an identity can guide behavior to move persons into structural positions across institutional domains. PMID:28364837

Haploidentical Hematopoietic Stem Cell Transplantation as Platform for Post-transplant Cellular Therapy

PubMed Central

Kongtim, Piyanuch; Lee, Dean A.; Cooper, Laurence J. N.; Kebriaei, Partow; Champlin, Richard E.; Ciurea, Stefan O.

2016-01-01

Haploidentical transplantation can extend the opportunity for transplantation to almost all patients who lack an HLA-matched donor. Advances in the field of haploidentical transplantation have led to a marked decrease in treatment-related mortality, allowing investigators to focus on developing rationale pre- and peri-remission therapies aimed at preventing disease relapse post-transplant. Due to widespread availability, low treatment-related mortality and cost, haploidentical donors may become the preferred “alternative” donors for allogeneic hematopoietic stem cell transplantation. One of the major advantages of using a related donor is the possibility to collect or generate additional cellular products from the same immediate available donor, which will not be rejected. Infusion of these cells in the peri-transplant period, derived from the same immune system, is opening the possibility to markedly enhance the anti-tumor effects of the graft and hasten immunologic reconstitution post-transplant. PMID:26172479

Stem-like plasticity and heterogeneity of circulating tumor cells: current status and prospect challenges in liver cancer

PubMed Central

Correnti, Margherita; Raggi, Chiara

2017-01-01

Poor prognosis and high recurrence remain leading causes of primary liver cancerassociated mortality. The spread of circulating tumor cells (CTCs) in the blood plays a major role in the initiation of metastasis and tumor recurrence after surgery. Nevertheless, only a subset of CTCs can survive, migrate to distant sites and establish secondary tumors. Consistent with cancer stem cell (CSC) hypothesis, stem-like CTCs might represent a potential source for cancer relapse and distant metastasis. Thus, identification of stem-like metastasis-initiating CTC-subset may provide useful clinically prognostic information. This review will emphasize the most relevant findings of CTCs in the context of stem-like biology associated to liver carcinogenesis. In this view, the emerging field of stem-like CTCs may deliver substantial contribution in liver cancer field in order to move to personalized approaches for diagnosis, prognosis and therapy. PMID:27738343

Setting FIRES to Stem Cell Research

ERIC Educational Resources Information Center

Miller, Roxanne Grietz

2005-01-01

The goal of this lesson is to present the basic scientific knowledge about stem cells, the promise of stem cell research to medicine, and the ethical considerations and arguments involved. One of the challenges of discussing stem cell research is that the field is constantly evolving and the most current information changes almost daily. Few…

The Influence of Proactive Personality and Coping on Commitment to STEM Majors

ERIC Educational Resources Information Center

Major, Debra A.; Holland, Jonathan M.; Oborn, Kurt L.

2012-01-01

Despite increasing demand for workers in fields that are grounded in science, technology, engineering, and math (STEM), retention rates are low among relevant college majors. Using Web-based survey data from 290 STEM majors, the authors investigated links among personality, coping strategies, and STEM major commitment. Proactive personality was…

Students with Disabilities' Perspectives of STEM Content and Careers

ERIC Educational Resources Information Center

Davis, Kimberly E. Bryant

2014-01-01

The current global economy has led to an increase in the need for workers in the fields of science, technology, engineering, and mathematics (STEM). Underrepresented populations, such as students with disabilities, are being considered as one way meet the STEM workforce shortages. In order to increase students' participation in STEM careers, it…

The stem heat balance method to measure transpiration:Evaluation of a new sensor

USDA-ARS?s Scientific Manuscript database

The direct measurement of crop transpiration (Tcrop) under field conditions and throughout the growing season is difficult to obtain. An available method uses stem flow gauge sensors, based on the stem heat balance. The sensor consists of a small heater that is wrapped around the stem of the plant a...

Effect of nitrogen fertilization and fungicides on Botryosphaeria stem blight lesion development on detached blueberry stems

USDA-ARS?s Scientific Manuscript database

Botryosphaeria stem blight is a destructive disease of blueberries. Field observations indicate stem blight is more severe on vigorous plants than on slower growing plants. Two studies compared the effect of two types of fertilizers applied at four rates and nine fungicides on lesion development fo...

STEM Attrition among High-Performing College Students in the United States: Scope and Potential Causes

ERIC Educational Resources Information Center

Chen, Xianglei

2015-01-01

Postsecondary education plays a critical role in building a strong workforce in Science, Technology, Engineering, and Mathematics (STEM) fields. The U.S. postsecondary education system, however, frequently loses many potential STEM graduates through attrition. An increasing portion of STEM leavers are top performers who might have made valuable…

Computing Galois Groups of Eisenstein Polynomials Over P-adic Fields

NASA Astrophysics Data System (ADS)

Milstead, Jonathan

The most efficient algorithms for computing Galois groups of polynomials over global fields are based on Stauduhar's relative resolvent method. These methods are not directly generalizable to the local field case, since they require a field that contains the global field in which all roots of the polynomial can be approximated. We present splitting field-independent methods for computing the Galois group of an Eisenstein polynomial over a p-adic field. Our approach is to combine information from different disciplines. We primarily, make use of the ramification polygon of the polynomial, which is the Newton polygon of a related polynomial. This allows us to quickly calculate several invariants that serve to reduce the number of possible Galois groups. Algorithms by Greve and Pauli very efficiently return the Galois group of polynomials where the ramification polygon consists of one segment as well as information about the subfields of the stem field. Second, we look at the factorization of linear absolute resolvents to further narrow the pool of possible groups.

Gendered Expectations: Examining How Peers Shape Female Students' Intent to Pursue STEM Fields.

PubMed

Riegle-Crumb, Catherine; Morton, Karisma

2017-01-01

Building on prior psychological and sociological research on the power of local environments to shape gendered outcomes in STEM fields, this study focuses on the critical stage of adolescence to explore the potential negative impact of exposure to exclusionary messages from peers within girls' science classrooms, as well as the positive potential impact of inclusionary messages. Specifically, utilizing longitudinal data from a diverse sample of adolescent youth, analyses examine how the presence of biased male peers, as well as confident female peers, shape girls' subsequent intentions to pursue different STEM fields, focusing specifically on intentions to pursue the male-dominated fields of computer science and engineering, as well as more gender equitable fields. Results reveal that exposure to a higher percentage of 8th grade male peers in the classroom who endorsed explicit gender/STEM stereotypes significantly and negatively predicted girls' later intentions to pursue a computer science/engineering (CS/E) major. Yet results also reveal that exposure to a higher percentage of confident female peers in the science classroom positively predicted such intentions. These results were specific to CS/E majors, suggesting that peers are an important source of messages regarding whether or not girls should pursue non-traditional STEM fields. This study calls attention to the importance of examining both positive and negative sources of influence within the local contexts where young people live and learn. Limitations and directions for future research are also discussed.

Gendered Expectations: Examining How Peers Shape Female Students' Intent to Pursue STEM Fields

PubMed Central

Riegle-Crumb, Catherine; Morton, Karisma

2017-01-01

Building on prior psychological and sociological research on the power of local environments to shape gendered outcomes in STEM fields, this study focuses on the critical stage of adolescence to explore the potential negative impact of exposure to exclusionary messages from peers within girls' science classrooms, as well as the positive potential impact of inclusionary messages. Specifically, utilizing longitudinal data from a diverse sample of adolescent youth, analyses examine how the presence of biased male peers, as well as confident female peers, shape girls' subsequent intentions to pursue different STEM fields, focusing specifically on intentions to pursue the male-dominated fields of computer science and engineering, as well as more gender equitable fields. Results reveal that exposure to a higher percentage of 8th grade male peers in the classroom who endorsed explicit gender/STEM stereotypes significantly and negatively predicted girls' later intentions to pursue a computer science/engineering (CS/E) major. Yet results also reveal that exposure to a higher percentage of confident female peers in the science classroom positively predicted such intentions. These results were specific to CS/E majors, suggesting that peers are an important source of messages regarding whether or not girls should pursue non-traditional STEM fields. This study calls attention to the importance of examining both positive and negative sources of influence within the local contexts where young people live and learn. Limitations and directions for future research are also discussed. PMID:28360868

Potential feasibility of dental stem cells for regenerative therapies: stem cell transplantation and whole-tooth engineering.

PubMed

Nakahara, Taka

2011-07-01

Multipotent mesenchymal stem cells from bone marrow are expected to be a somatic stem cell source for the development of new cell-based therapy in regenerative medicine. However, dental clinicians are unlikely to carry out autologous cell/tissue collection from patients (i.e., marrow aspiration) as a routine procedure in their clinics; hence, the utilization of bone marrow stem cells seems impractical in the dental field. Dental tissues harvested from extracted human teeth are well known to contain highly proliferative and multipotent stem cell compartments and are considered to be an alternative autologous cell source in cell-based medicine. This article provides a short overview of the ongoing studies for the potential application of dental stem cells and suggests the utilization of 2 concepts in future regenerative medicine: (1) dental stem cell-based therapy for hepatic and other systemic diseases and (2) tooth replacement therapy using the bioengineered human whole tooth, called the "test-tube dental implant." Regenerative therapies will bring new insights and benefits to the fields of clinical medicine and dentistry.

Women are underrepresented in fields where success is believed to require brilliance.

PubMed

Meyer, Meredith; Cimpian, Andrei; Leslie, Sarah-Jane

2015-01-01

Women's underrepresentation in science, technology, engineering, and mathematics (STEM) fields is a prominent concern in our society and many others. Closer inspection of this phenomenon reveals a more nuanced picture, however, with women achieving parity with men at the Ph.D. level in certain STEM fields, while also being underrepresented in some non-STEM fields. It is important to consider and provide an account of this field-by-field variability. The field-specific ability beliefs (FAB) hypothesis aims to provide such an account, proposing that women are likely to be underrepresented in fields thought to require raw intellectual talent-a sort of talent that women are stereotyped to possess less of than men. In two studies, we provide evidence for the FAB hypothesis, demonstrating that the academic fields believed by laypeople to require brilliance are also the fields with lower female representation. We also found that the FABs of participants with college-level exposure to a field were more predictive of its female representation than those of participants without college exposure, presumably because the former beliefs mirror more closely those of the field's practitioners (the direct "gatekeepers"). Moreover, the FABs of participants with college exposure to a field predicted the magnitude of the field's gender gap above and beyond their beliefs about the level of mathematical and verbal skills required. Finally, we found that beliefs about the importance of brilliance to success in a field may predict its female representation in part by fostering the impression that the field demands solitary work and competition with others. These results suggest new solutions for enhancing diversity within STEM and across the academic spectrum.

Biochemistry and biology: heart-to-heart to investigate cardiac progenitor cells.

PubMed

Chimenti, Isotta; Forte, Elvira; Angelini, Francesco; Messina, Elisa; Giacomello, Alessandro

2013-02-01

Cardiac regenerative medicine is a rapidly evolving field, with promising future developments for effective personalized treatments. Several stem/progenitor cells are candidates for cardiac cell therapy, and emerging evidence suggests how multiple metabolic and biochemical pathways strictly regulate their fate and renewal. In this review, we will explore a selection of areas of common interest for biology and biochemistry concerning stem/progenitor cells, and in particular cardiac progenitor cells. Numerous regulatory mechanisms have been identified that link stem cell signaling and functions to the modulation of metabolic pathways, and vice versa. Pharmacological treatments and culture requirements may be exploited to modulate stem cell pluripotency and self-renewal, possibly boosting their regenerative potential for cell therapy. Mitochondria and their many related metabolites and messengers, such as oxygen, ROS, calcium and glucose, have a crucial role in regulating stem cell fate and the balance of their functions, together with many metabolic enzymes. Furthermore, protein biochemistry and proteomics can provide precious clues on the definition of different progenitor cell populations, their physiology and their autocrine/paracrine regulatory/signaling networks. Interdisciplinary approaches between biology and biochemistry can provide productive insights on stem/progenitor cells, allowing the development of novel strategies and protocols for effective cardiac cell therapy clinical translation. This article is part of a Special Issue entitled Biochemistry of Stem Cells. Copyright © 2012 Elsevier B.V. All rights reserved.

Imaging of high-angle annular dark-field scanning transmission electron microscopy and observations of GaN-based violet laser diodes.

PubMed

Shiojiri, M; Saijo, H

2006-09-01

The first part of this paper is devoted to physics, to explain high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) imaging and to interpret why HAADF-STEM imaging is incoherent, instructing a strict definition of interference and coherence of electron waves. Next, we present our recent investigations of InGaN/GaN multiple quantum wells and AlGaN/GaN strained-layer superlattice claddings in GaN-based violet laser diodes, which have been performed by HAADF-STEM and high-resolution field-emission gun scanning electron microscopy.

Ethical aspects of tissue engineering: a review.

PubMed

de Vries, Rob B M; Oerlemans, Anke; Trommelmans, Leen; Dierickx, Kris; Gordijn, Bert

2008-12-01

Tissue engineering (TE) is a promising new field of medical technology. However, like other new technologies, it is not free of ethical challenges. Identifying these ethical questions at an early stage is not only part of science's responsibility toward society, but also in the interest of the field itself. In this review, we map which ethical issues related to TE have already been documented in the scientific literature. The issues that turn out to dominate the debate are the use of human embryonic stem cells and therapeutic cloning. Nevertheless, a variety of other ethical aspects are mentioned, which relate to different phases in the development of the field. In addition, we discuss a number of ethical issues that have not yet been raised in the literature.

The Diaporthe sojae species complex: Phylogenetic re-assessment of pathogens associated with soybean, cucurbits and other field crops.

PubMed

Udayanga, Dhanushka; Castlebury, Lisa A; Rossman, Amy Y; Chukeatirote, Ekachai; Hyde, Kevin D

2015-05-01

Phytopathogenic species of Diaporthe are associated with a number of soybean diseases including seed decay, pod and stem blight and stem canker and lead to considerable crop production losses worldwide. Accurate morphological identification of the species that cause these diseases has been difficult. In this study, we determined the phylogenetic relationships and species boundaries of Diaporthe longicolla, Diaporthe phaseolorum, Diaporthe sojae and closely related taxa. Species boundaries for this complex were determined based on combined phylogenetic analysis of five gene regions: partial sequences of calmodulin (CAL), beta-tubulin (TUB), histone-3 (HIS), translation elongation factor 1-α (EF1-α), and the nuclear ribosomal internal transcribed spacers (ITS). Phylogenetic analyses revealed that this large complex of taxa is comprised of soybean pathogens as well as species associated with herbaceous field crops and weeds. Diaporthe arctii, Diaporthe batatas, D. phaseolorum and D. sojae are epitypified. The seed decay pathogen D. longicolla was determined to be distinct from D. sojae. D. phaseolorum, originally associated with stem and leaf blight of Lima bean, was not found to be associated with soybean. A new species, Diaporthe ueckerae on Cucumis melo, is introduced with description and illustrations. Published by Elsevier Ltd.

Effects of Electromagnetic Fields on Osteogenesis of Human Alveolar Bone-Derived Mesenchymal Stem Cells

PubMed Central

Lim, KiTaek; Hexiu, Jin; Kim, Jangho; Seonwoo, Hoon; Cho, Woo Jae; Choung, Pill-Hoon; Chung, Jong Hoon

2013-01-01

This study was performed to investigate the effects of extremely low frequency pulsed electromagnetic fields (ELF-PEMFs) on the proliferation and differentiation of human alveolar bone-derived mesenchymal stem cells (hABMSCs). Osteogenesis is a complex series of events involving the differentiation of mesenchymal stem cells to generate new bone. In this study, we examined not merely the effect of ELF-PEMFs on cell proliferation, alkaline phosphatase (ALP) activity, and mineralization of the extracellular matrix but vinculin, vimentin, and calmodulin (CaM) expressions in hABMSCs during osteogenic differentiation. Exposure of hABMSCs to ELF-PEMFs increased proliferation by 15% compared to untreated cells at day 5. In addition, exposure to ELF-PEMFs significantly increased ALP expression during the early stages of osteogenesis and substantially enhanced mineralization near the midpoint of osteogenesis within 2 weeks. ELF-PEMFs also increased vinculin, vimentin, and CaM expressions, compared to control. In particular, CaM indicated that ELF-PEMFs significantly altered the expression of osteogenesis-related genes. The results indicated that ELF-PEMFs could enhance early cell proliferation in hABMSCs-mediated osteogenesis and accelerate the osteogenesis. PMID:23862141

Stem Cell Trials for Cardiovascular Medicine: Ethical Rationale

PubMed Central

Teraa, Martin; Hesam, Husna; van Delden, Johannes J.M.; Verhaar, Marianne C.; Bredenoord, Annelien L.

2014-01-01

Stem cell-based interventions provide new treatment prospects for many disease conditions, including cardiovascular disorders. Clinical trials are necessary to collect adequate evidence on (long-term) safety and efficacy of novel interventions such as stem cells, but the design and launch of clinical trials, from first-in-human studies to larger randomized controlled trials (RCTs), is scientifically and ethically challenging. Stem cells are different from traditional pharmaceuticals, surgical procedures, and medical devices in the following ways: the novelty and complexity of stem cells, the invasiveness of the procedures, and the novel aim of regeneration. These specifics, combined with the characteristics of the study population, will have an impact on the design and ethics of RCTs. The recently closed JUVENTAS trial will serve as an example to identify the (interwoven) scientific and ethical challenges in the design and launch of stem cell RCTs. The JUVENTAS trial has investigated the efficacy of autologous bone marrow cells in end-stage vascular patients, in a double-blind sham-controlled design. We first describe the choices, considerations, and experiences of the JUVENTAS team. Subsequently, we identify the main ethical and scientific challenges and discuss what is important to consider in the design of future stem cell RCTs: assessment of risks and benefits, the choice for outcome measures, the choice for the comparator, the appropriate selection of participants, and adequate informed consent. Additionally, the stem cell field is highly in the spotlight due to the (commercial) interests and expectations. This warrants a cautious pace of translation and scrupulous set up of clinical trials, as failures could put the field in a negative light. At the same time, knowledge from clinical trials is necessary for the field to progress. We conclude that in the scientifically and ethically challenging field of stem cell RCTs, researchers and clinicians have to maneuver between the Skylla of hyper accelerated translation without rigorously conducted RCTs and the Charybdis of the missed opportunity of valuable knowledge. PMID:24164351

A STEM Exploration with Gears

ERIC Educational Resources Information Center

Deis, Timothy; Julius, Julie

2017-01-01

Science, engineering, and mathematics are fields that many students see as separate entities. But if these fields are combined with technology, they become STEM. This investigation provides a context and allows students to explore mathematics, science, and engineering within that context. It requires students to model with mathematics and find…

CTE's Role in Science, Technology, Engineering and Mathematics

ERIC Educational Resources Information Center

Hyslop, Alisha

2010-01-01

For the last several years, concern has been brewing about America's underinvestment and underperformance in science, technology, engineering and mathematics--the fields collectively known as STEM. STEM can be described as an initiative for securing America's leadership in science, technology, engineering and mathematics fields and identifying…

Students drop out of STEM fields due to poor grades

NASA Astrophysics Data System (ADS)

Balcerak, Ernie

2013-09-01

College students planning to major in science, technology, engineering, and mathematics (STEM) fields often drop out of those fields because of poorer than expected grades, according to a recent study. Conducted by Ralph Stinebrickner of Berea College in Kentucky and Todd Stinebrickner of the University of Western Ontario, the study is a National Bureau of Economic Research working paper published in June 2013.

AccessSTEM: Building Capacity

ERIC Educational Resources Information Center

DO-IT, 2009

2009-01-01

A series of activities were undertaken to understand the underrepresentation of people with disabilities in science, technology, engineering, and mathematics (STEM) careers and increase their participation in these fields. "AccessSTEM" collaborated with key stakeholders to conduct a "Capacity-Building Institute"…

Atomic electric fields revealed by a quantum mechanical approach to electron picodiffraction.

PubMed

Müller, Knut; Krause, Florian F; Béché, Armand; Schowalter, Marco; Galioit, Vincent; Löffler, Stefan; Verbeeck, Johan; Zweck, Josef; Schattschneider, Peter; Rosenauer, Andreas

2014-12-15

By focusing electrons on probes with a diameter of 50 pm, aberration-corrected scanning transmission electron microscopy (STEM) is currently crossing the border to probing subatomic details. A major challenge is the measurement of atomic electric fields using differential phase contrast (DPC) microscopy, traditionally exploiting the concept of a field-induced shift of diffraction patterns. Here we present a simplified quantum theoretical interpretation of DPC. This enables us to calculate the momentum transferred to the STEM probe from diffracted intensities recorded on a pixel array instead of conventional segmented bright-field detectors. The methodical development yielding atomic electric field, charge and electron density is performed using simulations for binary GaN as an ideal model system. We then present a detailed experimental study of SrTiO3 yielding atomic electric fields, validated by comprehensive simulations. With this interpretation and upgraded instrumentation, STEM is capable of quantifying atomic electric fields and high-contrast imaging of light atoms.

Atomic electric fields revealed by a quantum mechanical approach to electron picodiffraction

NASA Astrophysics Data System (ADS)

Müller, Knut; Krause, Florian F.; Béché, Armand; Schowalter, Marco; Galioit, Vincent; Löffler, Stefan; Verbeeck, Johan; Zweck, Josef; Schattschneider, Peter; Rosenauer, Andreas

2014-12-01

By focusing electrons on probes with a diameter of 50 pm, aberration-corrected scanning transmission electron microscopy (STEM) is currently crossing the border to probing subatomic details. A major challenge is the measurement of atomic electric fields using differential phase contrast (DPC) microscopy, traditionally exploiting the concept of a field-induced shift of diffraction patterns. Here we present a simplified quantum theoretical interpretation of DPC. This enables us to calculate the momentum transferred to the STEM probe from diffracted intensities recorded on a pixel array instead of conventional segmented bright-field detectors. The methodical development yielding atomic electric field, charge and electron density is performed using simulations for binary GaN as an ideal model system. We then present a detailed experimental study of SrTiO3 yielding atomic electric fields, validated by comprehensive simulations. With this interpretation and upgraded instrumentation, STEM is capable of quantifying atomic electric fields and high-contrast imaging of light atoms.

Atomic electric fields revealed by a quantum mechanical approach to electron picodiffraction

PubMed Central

Müller, Knut; Krause, Florian F.; Béché, Armand; Schowalter, Marco; Galioit, Vincent; Löffler, Stefan; Verbeeck, Johan; Zweck, Josef; Schattschneider, Peter; Rosenauer, Andreas

2014-01-01

By focusing electrons on probes with a diameter of 50 pm, aberration-corrected scanning transmission electron microscopy (STEM) is currently crossing the border to probing subatomic details. A major challenge is the measurement of atomic electric fields using differential phase contrast (DPC) microscopy, traditionally exploiting the concept of a field-induced shift of diffraction patterns. Here we present a simplified quantum theoretical interpretation of DPC. This enables us to calculate the momentum transferred to the STEM probe from diffracted intensities recorded on a pixel array instead of conventional segmented bright-field detectors. The methodical development yielding atomic electric field, charge and electron density is performed using simulations for binary GaN as an ideal model system. We then present a detailed experimental study of SrTiO3 yielding atomic electric fields, validated by comprehensive simulations. With this interpretation and upgraded instrumentation, STEM is capable of quantifying atomic electric fields and high-contrast imaging of light atoms. PMID:25501385

Ethical and Safety Issues of Stem Cell-Based Therapy.

PubMed

Volarevic, Vladislav; Markovic, Bojana Simovic; Gazdic, Marina; Volarevic, Ana; Jovicic, Nemanja; Arsenijevic, Nebojsa; Armstrong, Lyle; Djonov, Valentin; Lako, Majlinda; Stojkovic, Miodrag

2018-01-01

Results obtained from completed and on-going clinical studies indicate huge therapeutic potential of stem cell-based therapy in the treatment of degenerative, autoimmune and genetic disorders. However, clinical application of stem cells raises numerous ethical and safety concerns. In this review, we provide an overview of the most important ethical issues in stem cell therapy, as a contribution to the controversial debate about their clinical usage in regenerative and transplantation medicine. We describe ethical challenges regarding human embryonic stem cell (hESC) research, emphasizing that ethical dilemma involving the destruction of a human embryo is a major factor that may have limited the development of hESC-based clinical therapies. With previous derivation of induced pluripotent stem cells (iPSCs) this problem has been overcome, however current perspectives regarding clinical translation of iPSCs still remain. Unlimited differentiation potential of iPSCs which can be used in human reproductive cloning, as a risk for generation of genetically engineered human embryos and human-animal chimeras, is major ethical issue, while undesired differentiation and malignant transformation are major safety issues. Although clinical application of mesenchymal stem cells (MSCs) has shown beneficial effects in the therapy of autoimmune and chronic inflammatory diseases, the ability to promote tumor growth and metastasis and overestimated therapeutic potential of MSCs still provide concerns for the field of regenerative medicine. This review offers stem cell scientists, clinicians and patient's useful information and could be used as a starting point for more in-depth analysis of ethical and safety issues related to clinical application of stem cells.

Stem cells and bone: a historical perspective.

PubMed

Bianco, Paolo

2015-01-01

Bone physiology and stem cells were tightly intertwined with one another, both conceptually and experimentally, long before the current explosion of interest in stem cells and so-called regenerative medicine. Bone is home to the two best known and best characterized systems of postnatal stem cells, and it is the only organ in which two stem cells and their dependent lineages coordinate the overall adaptive responses of two major physiological systems. All along, the nature and the evolutionary significance of the interplay of bone and hematopoiesis have remained a major scientific challenge, but also allowed for some of the most spectacular developments in cell biology-based medicine, such as hematopoietic stem cell transplantation. This question recurs in novel forms at multiple turning points over time: today, it finds in the biology of the "niche" its popular phrasing. Entirely new avenues of investigation emerge as a new view of bone in physiology and medicine is progressively established. Looking at bone and stem cells in a historical perspective provides a unique case study to highlight the general evolution of science in biomedicine since the end of World War II to the present day. A paradigm shift in science and in its relation to society and policies occurred in the second half of the XXth century, with major implications thereof for health, industry, drug development, market and society. Current interest in stem cells in bone as in other fields is intertwined with that shift. New opportunities and also new challenges arise. This article is part of a Special Issue entitled "Stem cells and bone". Copyright © 2014. Published by Elsevier Inc.

Ethical and Safety Issues of Stem Cell-Based Therapy

PubMed Central

Volarevic, Vladislav; Markovic, Bojana Simovic; Gazdic, Marina; Volarevic, Ana; Jovicic, Nemanja; Arsenijevic, Nebojsa; Armstrong, Lyle; Djonov, Valentin; Lako, Majlinda; Stojkovic, Miodrag

2018-01-01

Results obtained from completed and on-going clinical studies indicate huge therapeutic potential of stem cell-based therapy in the treatment of degenerative, autoimmune and genetic disorders. However, clinical application of stem cells raises numerous ethical and safety concerns. In this review, we provide an overview of the most important ethical issues in stem cell therapy, as a contribution to the controversial debate about their clinical usage in regenerative and transplantation medicine. We describe ethical challenges regarding human embryonic stem cell (hESC) research, emphasizing that ethical dilemma involving the destruction of a human embryo is a major factor that may have limited the development of hESC-based clinical therapies. With previous derivation of induced pluripotent stem cells (iPSCs) this problem has been overcome, however current perspectives regarding clinical translation of iPSCs still remain. Unlimited differentiation potential of iPSCs which can be used in human reproductive cloning, as a risk for generation of genetically engineered human embryos and human-animal chimeras, is major ethical issue, while undesired differentiation and malignant transformation are major safety issues. Although clinical application of mesenchymal stem cells (MSCs) has shown beneficial effects in the therapy of autoimmune and chronic inflammatory diseases, the ability to promote tumor growth and metastasis and overestimated therapeutic potential of MSCs still provide concerns for the field of regenerative medicine. This review offers stem cell scientists, clinicians and patient's useful information and could be used as a starting point for more in-depth analysis of ethical and safety issues related to clinical application of stem cells. PMID:29333086

Improving and accelerating the differentiation and functional maturation of human stem cell-derived neurons: role of extracellular calcium and GABA.

PubMed

Kemp, Paul J; Rushton, David J; Yarova, Polina L; Schnell, Christian; Geater, Charlene; Hancock, Jane M; Wieland, Annalena; Hughes, Alis; Badder, Luned; Cope, Emma; Riccardi, Daniela; Randall, Andrew D; Brown, Jonathan T; Allen, Nicholas D; Telezhkin, Vsevolod

2016-11-15

Neurons differentiated from pluripotent stem cells using established neural culture conditions often exhibit functional deficits. Recently, we have developed enhanced media which both synchronize the neurogenesis of pluripotent stem cell-derived neural progenitors and accelerate their functional maturation; together these media are termed SynaptoJuice. This pair of media are pro-synaptogenic and generate authentic, mature synaptic networks of connected forebrain neurons from a variety of induced pluripotent and embryonic stem cell lines. Such enhanced rate and extent of synchronized maturation of pluripotent stem cell-derived neural progenitor cells generates neurons which are characterized by a relatively hyperpolarized resting membrane potential, higher spontaneous and induced action potential activity, enhanced synaptic activity, more complete development of a mature inhibitory GABA A receptor phenotype and faster production of electrical network activity when compared to standard differentiation media. This entire process - from pre-patterned neural progenitor to active neuron - takes 3 weeks or less, making it an ideal platform for drug discovery and disease modelling in the fields of human neurodegenerative and neuropsychiatric disorders, such as Huntington's disease, Parkinson's disease, Alzheimer's disease and Schizophrenia. © 2016 The Authors. The Journal of Physiology © 2016 The Physiological Society.

Stem Cell Therapies in Retinal Disorders.

PubMed

Garg, Aakriti; Yang, Jin; Lee, Winston; Tsang, Stephen H

2017-02-02

Stem cell therapy has long been considered a promising mode of treatment for retinal conditions. While human embryonic stem cells (ESCs) have provided the precedent for regenerative medicine, the development of induced pluripotent stem cells (iPSCs) revolutionized this field. iPSCs allow for the development of many types of retinal cells, including those of the retinal pigment epithelium, photoreceptors, and ganglion cells, and can model polygenic diseases such as age-related macular degeneration. Cellular programming and reprogramming technology is especially useful in retinal diseases, as it allows for the study of living cells that have genetic variants that are specific to patients' diseases. Since iPSCs are a self-renewing resource, scientists can experiment with an unlimited number of pluripotent cells to perfect the process of targeted differentiation, transplantation, and more, for personalized medicine. Challenges in the use of stem cells are present from the scientific, ethical, and political realms. These include transplant complications leading to anatomically incorrect placement, concern for tumorigenesis, and incomplete targeting of differentiation leading to contamination by different types of cells. Despite these limitations, human ESCs and iPSCs specific to individual patients can revolutionize the study of retinal disease and may be effective therapies for conditions currently considered incurable.

Studies of local polarization in complex oxide multiferroic interfaces by aberration corrected STEM-EELS

NASA Astrophysics Data System (ADS)

Sanchez-Santolino, Gabriel; Tornos, Javier; Leon, Carlos; Varela, María; Pennycook, Stephen J.; Santamaría, Jacobo

2014-03-01

Interfaces in complex oxide heterostructures are responsible for exciting new physics, which is directly related to the chemical, structural and electronic properties at the atomic scale. Here, we study artificial multiferroic heterostructures combining ferromagnetic La0.7Sr0.3MnO3 with ferroelectric BaTiO3 by atomic resolution aberration-corrected scanning transmission electron microscopy (STEM) and electron energy-loss spectroscopy. Measurements of the atomic positions in the STEM images permit calculating relative displacements and hence, local polarization. Polarization gradients can be observed in annular bright field images which seem to be correlated to strain gradients associated with the large lattice mismatch between barriers and electrodes. Spectroscopic measurements suggest the presence of O vacancies through the ferroelectric layers. Understanding the effect of the charge carriers associated with the oxygen vacancies may be the key to control the dynamics of domain walls in these heterostructures. Acknowledgements ORNL: U.S. DOE-BES, Materials Sciences and Engineering Division. UCM: ERC Starting Investigator Award, Spanish MICINN MAT2011-27470-C02 and Consolider Ingenio 2010 - CSD2009-00013 (Imagine), CAM S2009/MAT-1756 (Phama).

Self-Definition of Women Experiencing a Nontraditional Graduate Fellowship Program

ERIC Educational Resources Information Center

Buck, Gayle A.; Leslie-Pelecky, Diandra L.; Lu, Yun; Plano Clark, Vicki L.; Creswell, John W.

2006-01-01

Women continue to be underrepresented in the fields of science, technology, engineering, and mathematics (STEM). One factor contributing to this underrepresentation is the graduate school experience. Graduate programs in STEM fields are constructed around assumptions that ignore the reality of women's lives; however, emerging opportunities may…

ADVANCing the Agenda for Gender Equity

ERIC Educational Resources Information Center

Laursen, Sandra L.; Austin, Ann E.; Soto, Melissa; Martinez, Dalinda

2015-01-01

In recent years, women's representation in the science, technology, engineering, and mathematics (STEM) fields has grown at the undergraduate level, with STEM degrees earned by US women reaching parity in some fields and making notable progress in others. Yet the faculty with whom these undergraduates interact in classes and labs are much less…

Voices from the Past: Messages for a STEM Future

ERIC Educational Resources Information Center

Kelley, Todd R.

2012-01-01

The current emphasis in K-12 education on science, technology, engineering, and mathematics (STEM) (Douglas, Iversen, & Kalyandurg, 2004; Sanders, 2009) creates many ways to partner engineering education with these fields. Therefore, it is appropriate to examine the commonalities these fields have with engineering education. Though much of the…

Regenerative Rehabilitation: Applied Biophysics Meets Stem Cell Therapeutics.

PubMed

Rando, Thomas A; Ambrosio, Fabrisia

2018-03-01

The emerging field of regenerative rehabilitation integrates biological and bioengineering advances in regenerative medicine with rehabilitative sciences. Here we highlight recent stem cell-based examples of the regenerative rehabilitation paradigm to promote tissue repair and regeneration, and we discuss remaining challenges and future directions for the field. Published by Elsevier Inc.

Women are underrepresented in fields where success is believed to require brilliance

PubMed Central

Meyer, Meredith; Cimpian, Andrei; Leslie, Sarah-Jane

2015-01-01

Women’s underrepresentation in science, technology, engineering, and mathematics (STEM) fields is a prominent concern in our society and many others. Closer inspection of this phenomenon reveals a more nuanced picture, however, with women achieving parity with men at the Ph.D. level in certain STEM fields, while also being underrepresented in some non-STEM fields. It is important to consider and provide an account of this field-by-field variability. The field-specific ability beliefs (FAB) hypothesis aims to provide such an account, proposing that women are likely to be underrepresented in fields thought to require raw intellectual talent—a sort of talent that women are stereotyped to possess less of than men. In two studies, we provide evidence for the FAB hypothesis, demonstrating that the academic fields believed by laypeople to require brilliance are also the fields with lower female representation. We also found that the FABs of participants with college-level exposure to a field were more predictive of its female representation than those of participants without college exposure, presumably because the former beliefs mirror more closely those of the field’s practitioners (the direct “gatekeepers”). Moreover, the FABs of participants with college exposure to a field predicted the magnitude of the field’s gender gap above and beyond their beliefs about the level of mathematical and verbal skills required. Finally, we found that beliefs about the importance of brilliance to success in a field may predict its female representation in part by fostering the impression that the field demands solitary work and competition with others. These results suggest new solutions for enhancing diversity within STEM and across the academic spectrum. PMID:25814964

An Effective Model of Developing Teacher Leaders in STEM Education

ERIC Educational Resources Information Center

Sublette, Heidi

2013-01-01

In the last 5 years, industries have begun to recognize a growing gap in the production of college graduates in areas of STEM. Researchers in various industries believe this gap will create a significant loss of competitive edge in the STEM fields, which will leave the United States pursuing STEM graduates from foreign countries and may ultimately…

Public Dialogue with Science and Development for Teachers of STEM: Linking Public Dialogue with Pedagogic Praxis

ERIC Educational Resources Information Center

Watermeyer, Richard; Montgomery, Catherine

2018-01-01

Despite evidence of quality teaching in Science, Technology, Engineering and Mathematics (STEM) subject domains and insistence on the part of many national governments on the economic value of STEM, education, recruitment and retention into STEM subject fields and occupations is said to be continually blighted by a "leaky pipeline". In…

Increasing the Number of STEM Graduates: Insights from the U.S. STEM Education & Modeling Project

ERIC Educational Resources Information Center

Business-Higher Education Forum (NJ1), 2010

2010-01-01

The Business-Higher Education Forum's (BHEF's) Securing America's Leadership in STEM Initiative has broken new ground in addressing one of the nation's most critical challenges--increasing the number of students who are interested in and pursue careers in science, technology, engineering or mathematics, the so-called "STEM" fields. The…

The Role of Aspirational Experiences and Behaviors in Cultivating Momentum for Transfer Access in STEM: Variations across Gender and Race

ERIC Educational Resources Information Center

Wang, Xueli; Lee, Seo Young; Prevost, Amy

2017-01-01

Objective: Although upward transfer in science, technology, engineering, and mathematics (STEM) fields represents a prominent national policy concern, community college students' aspirations for transfer in STEM are often impeded, resulting in lower transfer rates. This study investigated four aspects of community college STEM students'…

Designing an Optical Instrument: A Culminating STEM Activity for a Primary Science Light Unit

ERIC Educational Resources Information Center

King, Donna; English, Lyn

2016-01-01

Nationally and internationally there have been calls for a focus on STEM (science, technology, engineering and mathematics) teaching and learning in schools to prepare students for the many future careers in the STEM fields. One way to do this is through engineering activities that provide opportunities for integrating STEM to solve problems using…

Dual enrollment as a factor for women transitioning into STEM majors in Montana two-year colleges

NASA Astrophysics Data System (ADS)

Jakes, Penny Jane

The purpose of this non-experimental, descriptive, quantitative study was to describe the impact high school dual enrollment coursework has had on initial enrollment of women with STEM majors in Montana two-year colleges. The study was designed to find whether or not differences existed for access (initial enrollment), persistence (to third semester), and success (associate's degree, certificate, or transfer to a four-year institution within 150% of program length). The literature review highlighted the need for studies to address the issue of few women in science, technology, engineering, and mathematics (STEM) occupations. One goal of dual enrollment in Montana is to ease transitions from high school to college, including underrepresented populations such as women in STEM fields. The scope of this study was to collect, organize, and interpret data to describe the effect of that effort for women enrolling in STEM majors at two-year colleges in Montana. Baseline information established the demographics of young women who participated in dual enrollment in Montana high schools during 2007-2009. Data analysis described results using attributes of gender, dual enrollment, access, persistence, and success for those enrolled in STEM fields. Results indicated more young women than young men take advantage of dual enrollment in high school and more women than men with dual-enrollment credit initially enroll in college. More men than women major in a STEM field and more men persist and graduate within the STEM fields. Data indicated that 221 students enrolled in a Montana two-year college with DE/DC credit during 2007-2009. Of those, eight women chose STEM majors, six persisted to the third semester, and two completed. It is recommended that a mixed-methods study be conducted to give a deeper level of understanding for enrollment trends and career choice. Longitudinal studies should also be conducted as dual enrollment grows within the state of Montana. Further studies would enable educational stakeholders to make informed decisions to create meaningful change for women in STEM majors.

High-angle annular dark field scanning transmission electron microscopy on carbon-based functional polymer systems.

PubMed

Sourty, Erwan; van Bavel, Svetlana; Lu, Kangbo; Guerra, Ralph; Bar, Georg; Loos, Joachim

2009-06-01

Two purely carbon-based functional polymer systems were investigated by bright-field conventional transmission electron microscopy (CTEM) and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM). For a carbon black (CB) filled polymer system, HAADF-STEM provides high contrast between the CB agglomerates and the polymer matrix so that details of the interface organization easily can be revealed and assignment of the CB phase is straightforward. For a second system, the functional polymer blend representing the photoactive layer of a polymer solar cell, details of its nanoscale organization could be observed that were not accessible with CTEM. By varying the camera length in HAADF-STEM imaging, the contrast can be enhanced between crystalline and amorphous compounds due to diffraction contrast so that nanoscale interconnections between domains are identified. In general, due to its incoherent imaging characteristics HAADF-STEM allows for reliable interpretation of the data obtained.

Psychosocial Pathways to STEM Engagement among Graduate Students in the Life Sciences.

PubMed

Clark, Sheri L; Dyar, Christina; Maung, Nina; London, Bonita

2016-01-01

Despite growing diversity among life sciences professionals, members of historically underrepresented groups (e.g., women) continue to encounter barriers to academic and career advancement, such as subtle messages and stereotypes that signal low value for women, and fewer opportunities for quality mentoring relationships. These barriers reinforce the stereotype that women's gender is incompatible with their science, technology, engineering, and mathematics (STEM) field, and can interfere with their sense of belonging and self-efficacy within STEM. The present work expands this literature in two ways, by 1) focusing on a distinct period in women's careers that has been relatively understudied, but represents a critical period when career decisions are made, that is, graduate school; and 2) highlighting the buffering effect of one critical mechanism against barriers to STEM persistence, that is, perceived support from advisors. Results of the present study show that perceived support from one's advisor may promote STEM engagement among women by predicting greater gender-STEM identity compatibility, which in turn predicts greater STEM importance among women (but not men). STEM importance further predicts higher sense of belonging in STEM for both men and women and increased STEM self-efficacy for women. Finally, we describe the implications of this work for educational policy. © 2016 S. L. Clark et al. CBE—Life Sciences Education © 2016 The American Society for Cell Biology. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

Limitation of Unloading in the Developing Grains Is a Possible Cause Responsible for Low Stem Non-structural Carbohydrate Translocation and Poor Grain Yield Formation in Rice through Verification of Recombinant Inbred Lines

PubMed Central

Li, Guohui; Pan, Junfeng; Cui, Kehui; Yuan, Musong; Hu, Qiuqian; Wang, Wencheng; Mohapatra, Pravat K.; Nie, Lixiao; Huang, Jianliang; Peng, Shaobing

2017-01-01

Remobilisation of non-structural carbohydrates (NSC) from leaves and stems and unloading into developing grains are essential for yield formation of rice. In present study, three recombinant inbred lines of rice, R91, R156 and R201 have been tested for source-flow-sink related attributes determining the nature of NSC accumulation and translocation at two nitrogen levels in the field. Compared to R91 and R156, R201 had lower grain filling percentage, harvest index, and grain yield. Meanwhile, R201 had significantly lower stem NSC translocation during grain filling stage. Grain filling percentage, harvest index, and grain yield showed the consistent trend with stem NSC translocation among the three lines. In comparison with R91 and R156, R201 had similarity in leaf area index, specific leaf weight, stem NSC concentration at heading, biomass, panicles m-2, spikelets per panicle, remobilization capability of assimilation in stems, sink capacity, sink activity, number and cross sectional area of small vascular bundles, greater number and cross sectional area of large vascular bundles, and higher SPAD, suggesting that source, flow, and sink were not the limiting factors for low stem NSC translocation and grain filling percentage of R201. However, R201 had significant higher stem and rachis NSC concentrations at maturity, which implied that unloading in the developing grains might result in low NSC translocation in R201. The results indicate that stem NSC translocation could be beneficial for enhancement of grain yield potential, and poor unloading into caryopsis may be the possible cause of low stem NSC translocation, poor grain filling and yield formation in R201. PMID:28848573

A generalized electro-elastic theory of polymer networks

NASA Astrophysics Data System (ADS)

Cohen, Noy

2018-01-01

A rigorous multi-scale analysis of the electromechanical coupling in dielectric polymers is conducted. The body couples stemming from a misalignment between the electric field and the electric-dipole density vector are studied and the conservation laws for polymer networks are derived. Using variational principles, expressions for the polarization and the stress are determined. Interestingly, it is found that the stress tensor resulting from coupled loadings in which the electric field is misaligned with the principal stretch directions is not symmetric and the asymmetry arises from the body couples. Next, the electro-mechanical response of a chain is analyzed. The deformations of the individual polymer chains are related to the macroscopic deformation via two highly non-linear constraints - the first pertaining to the compatibility of the local deformations with the imposed macroscopic one and the second stems from the symmetric part of the stress at equilibrium. In accord with the proposed framework, an amended three-chains model is introduced. The predictions of this model are found to be in excellent agreement with experimental findings. Lastly, the behavior of a polymer subjected to a simple shear and an electric field is studied. The offset between the electric field and the principal directions gives rise to body couples, a polarization that is not aligned with the electric field, and an asymmetric stress tensor.

Using Written Work to Investigate Stages in Sixth-Grade Students' Construction and Coordination of Units

ERIC Educational Resources Information Center

Ulrich, Catherine; Wilkins, Jesse L. M.

2017-01-01

Background: Students' ability to construct and coordinate units has been found to have far-reaching implications for their ability to develop sophisticated understandings of key middle-grade mathematical topics such as fractions, ratios, proportions, and algebra, topics that form the base of understanding for most STEM-related fields. Most of the…

The Use of De Jure to Maintain a De Facto Status Quo

ERIC Educational Resources Information Center

Gallard Martínez, Alejandro José; Antrop-González, René

2013-01-01

The vignette we use as the introduction works to define and distinguish the concepts of de jure and de facto pedagogical actions, especially as related to Latin@ education and its relationship with STEM fields. The authors assert that de jure educational policies, which are often legal guidelines that mandate minimum levels of compliance,…

Relative susceptibility of oaks to seven species of Phytophthora isolated from oak forest soils

Treesearch

Y. Balci; S. Balci; W.L. MacDonald; K.W. Gottschalk

2008-01-01

Isolates of Phytophthora cambivora, P. cinnamomi, P. citricola, P. europaea, P. quercetorum and two unidentified species were tested for their pathogenicity to eastern US oak species by root and stem inoculations. Experiments were conducted during two different periods and included 1-, 2- and 20- year-old oaks grown under greenhouse and field...

The Algebra Artist

ERIC Educational Resources Information Center

Beigie, Darin

2014-01-01

Most people who are attracted to STEM-related fields are drawn not by a desire to take mathematics tests but to create things. The opportunity to create an algebra drawing gives students a sense of ownership and adventure that taps into the same sort of energy that leads a young person to get lost in reading a good book, building with Legos®,…

Exploration of Factors Related to the Development of Science, Technology, Engineering, and Mathematics Graduate Teaching Assistants' Teaching Orientations

ERIC Educational Resources Information Center

Gilmore, Joanna; Maher, Michelle A.; Feldon, David F.; Timmerman, Briana

2014-01-01

Research indicates that modifying teachers' beliefs about learning and teaching (i.e. teaching orientation) may be a prerequisite to changing their teaching practices. This mixed methods study quantitized data from interviews with 65 graduate teaching assistants (GTAs) from science, technology, engineering, and mathematics (STEM) fields to assess…

Proteomic analysis of responsive stem proteins of resistant and susceptible cashew plants after Lasiodiplodia theobromae infection.

PubMed

Cipriano, Aline K A L; Gondim, Darcy M F; Vasconcelos, Ilka M; Martins, Jorge A M; Moura, Arlindo A; Moreno, Frederico B; Monteiro-Moreira, Ana C O; Melo, Jose G M; Cardoso, Jose E; Paiva, Ana Luiza S; Oliveira, Jose T A

2015-01-15

Gummosis is an aggressive disease caused by the necrotrophic fungus Lasiodiplodia theobromae (Pat.) Griffon & Maubl that threatens commercial cashew orchads in Brazil. To study the molecular mechanisms involved in the cashew response to L. theobromae, a proteomic analysis of stems from the commercial cashew clone BRS 226 (resistant) was conducted at early times post-artificial infection. In addition, changes in the stem proteome profiles of gummosis resistant and susceptible cashew plants grown under field condition and naturally exposed to pathogen were also compared. After two-dimensional gel electrophoresis (2D-PAGE), 73 proteins showed statistically significant differences in spot abundance. Of these, 31 spots were identified in BRS 226 stems compared with mock-inoculated controls and 32 in stems collected from field-grown resistant and susceptible cashew plants. L. theobromae-responsive proteins were mainly involved in energy metabolism pathways, stress and defense, cell signaling and protein metabolism indicating modulation of various cellular functions upon fungal infection. As stress-inducing factors seem to be important for susceptibility to disease, the change in the abundance relative these proteins may possibly indicate an attempt to maintain cellular homeostasis, as resistance determinant factor, related with a possible role in the regulation of oxidative burst. These findings provide the first information about the cellular mechanisms acting in the Anacardium occidentale genotypes associated with the pathophysiological state of infection with L. theobromae. Gummosis caused by Lasiodiplodia theobromae, a necrotrophic fungus, is the major disease of cashew plants in the semi-arid conditions of northeastern Brazil. Although various studies were carried out on this pathosystem, there is no information available on the molecular mechanisms of plant defense related to the incompatible interaction of cashew with L. theobromae. Therefore, this original study comprises a differential proteomic analysis of cashew stems from: (i) resistant dwarf clone BRS 226 mock-inoculated (control) and artificially inoculated with L. theobromae; and (ii) cashew plants bearing resistant and susceptible traits to gummosis, originated from open pollination of BRS 226 in a commercial orchard with high disease incidence. The contribution of the reprogrammed proteins to molecular events triggered in cashew plants challenged by L. theobromae has a great relevance in the identification of the host candidate proteins linked to biological pathways that respond to L. theobromae infection. Furthermore this study may contribute to improve breeding programs aimed at selecting resistant/tolerant cashew clones toward this pathogen. Copyright © 2014 Elsevier B.V. All rights reserved.

Engaging the Public Through an Interactive Astronomy Outreach Program

NASA Astrophysics Data System (ADS)

Thompson, Kristen

2018-01-01

The growing technology sector of the U.S. economy in an increasingly complex world has made it more important than ever for students to gather information, think critically, and solve problems. These skills are often acquired through the study of STEM disciplines. In an effort to inspire students and the public in the Charlotte, NC area to take an interest in STEM related fields, the Physics Department at Davidson College has recently developed an interactive astronomy community engagement program. This program is comprised of off-campus events that bring STEM programming to K-12 children, on-campus public star parties, and a day-long astronomy fair called Davidson Space Day. This presentation will illustrate the implementation of each of these components of our outreach program, present an evaluation of their success, and describe future goals and lessons learned thus far. This outreach program was made possible through funding from the NC Space Grant Consortium.

The use of de jure to maintain a de facto status quo

NASA Astrophysics Data System (ADS)

Gallard Martínez, Alejandro José; Antrop-González, René

2013-12-01

The vignette we use as the introduction works to define and distinguish the concepts of de jure and de facto pedagogical actions, especially as related to Latin@ education and its relationship with STEM fields. The authors assert that de jure educational policies, which are often legal guidelines that mandate minimum levels of compliance, unfortunately become translated to mean the normative way to implement educational practice. Hence, going above and beyond the call of duty to educate Latin@ children and youth through culturally meaningful STEM practices while respecting, affirming, and utilizing decolonizing ways of viewing science and math is not viewed as standard. Thus, it is imperative that STEM educators who work with Latin@ learners demand that de jure education guidelines translated as de facto pedagogical actions are not enough. On the contrary, de jure and de facto ways of teaching and learning should always consist of a counterhegemonic normative.

Computational Tools for Stem Cell Biology

PubMed Central

Bian, Qin; Cahan, Patrick

2016-01-01

For over half a century, the field of developmental biology has leveraged computation to explore mechanisms of developmental processes. More recently, computational approaches have been critical in the translation of high throughput data into knowledge of both developmental and stem cell biology. In the last several years, a new sub-discipline of computational stem cell biology has emerged that synthesizes the modeling of systems-level aspects of stem cells with high-throughput molecular data. In this review, we provide an overview of this new field and pay particular attention to the impact that single-cell transcriptomics is expected to have on our understanding of development and our ability to engineer cell fate. PMID:27318512

Computational Tools for Stem Cell Biology.

PubMed

Bian, Qin; Cahan, Patrick

2016-12-01

For over half a century, the field of developmental biology has leveraged computation to explore mechanisms of developmental processes. More recently, computational approaches have been critical in the translation of high throughput data into knowledge of both developmental and stem cell biology. In the past several years, a new subdiscipline of computational stem cell biology has emerged that synthesizes the modeling of systems-level aspects of stem cells with high-throughput molecular data. In this review, we provide an overview of this new field and pay particular attention to the impact that single cell transcriptomics is expected to have on our understanding of development and our ability to engineer cell fate. Copyright © 2016 Elsevier Ltd. All rights reserved.

Development and Application of STEM for the Biological Sciences

PubMed Central

Sousa, Alioscka A.; Leapman, Richard D.

2012-01-01

The design of the scanning transmission electron microscope (STEM), as conceived originally by Crewe and coworkers, enables the highly efficient and flexible collection of different elastic and inelastic signals resulting from the interaction of a focused probe of incident electrons with a specimen. In the present paper we provide a brief review for how the STEM today can be applied towards a range of different problems in the biological sciences, emphasizing four main areas of application. (1) For three decades, the most widely used STEM technique has been the mass determination of proteins and other macromolecular assemblies. Such measurements can be performed at low electron dose by collecting the high-angle dark-field signal using an annular detector. STEM mass mapping has proven valuable for characterizing large protein assemblies such as filamentous proteins with a well-defined mass per length. (2) The annular dark-field signal can also be used to image ultrasmall, functionalized nanoparticles of heavy atoms for labeling specific aminoacid sequences in protein assemblies. (3) By acquiring electron energy loss spectra (EELS) at each pixel in a hyperspectral image, it is possible to map the distributions of specific bound elements like phosphorus, calcium and iron in isolated macromolecular assemblies or in compartments within sectioned cells. Near single atom sensitivity is feasible provided that the specimen can tolerate a very high incident electron dose. (4) Electron tomography is a new application of STEM that enables three-dimensional reconstruction of micrometer-thick sections of cells. In this technique a probe of small convergence angle gives a large depth of field throughout the thickness of the specimen while maintaining a probe diameter of < 2 nm; and the use of an on-axis bright-field detector reduces the effects of beam broadening and thus improves the spatial resolution compared to that attainable by STEM dark-field tomography. PMID:22749213

Women planning to major in computer science: Who are they and what makes them unique?

NASA Astrophysics Data System (ADS)

Lehman, Kathleen J.; Sax, Linda J.; Zimmerman, Hilary B.

2016-12-01

Despite the current growing popularity of the computer science (CS) major, women remain sorely underrepresented in the field, continuing to earn only 18% of bachelor's degrees. Understanding women's low rates of participation in CS is important given that the demand for individuals with CS training has grown sharply in recent years. Attracting and retaining more women to high-paying fields like CS may also help narrow the gender pay gap. Further, it is important that women participate in developing new technology so that technology advances serve the needs of both women and men. This paper explores the background characteristics, career aspirations, and self-perceptions of 1636 female first-year college students in the United States who intend to major in CS and compares them with 4402 male CS aspirants as well as with 26,642 women planning to major in other STEM sub-fields. The findings reveal a unique profile of women who pursue the CS major and notes many significant differences between men and women in CS and between women in CS and those in other STEM fields. For instance, women in CS tend to earn lower high school grades than women in other STEM fields, but earn higher SAT verbal scores. They also rate themselves higher than men in CS and women in other STEM fields on measures of their artistic ability, but rate themselves lower on other self-ratings, including academic and leadership ability. Further, women in CS are more likely to be undecided in their career plans than men in CS and women in other STEM fields. Understanding the unique characteristics of women in CS will help inform policies and recruitment programs designed to address the gender gap in computing.

Potato stem density effects on canopy development and production

USDA-ARS?s Scientific Manuscript database

Controlled environment studies with potato frequently assume responses from single-stem potato plants can be extrapolated to the field where multiple-stem plants are common. Two controlled environment studies were conducted in order to characterize differences in canopy growth, development, and dry...

STEM Intervention Programs: Funding Practices and Challenges

ERIC Educational Resources Information Center

Rincon, Blanca E.; George-Jackson, Casey E.

2016-01-01

This study examines the funding practices and challenges of diversity initiatives found in the science, technology, engineering, and mathematics (STEM) fields. Interviews with 55 intervention program administrators, representing 48 unique STEM intervention programs, were conducted at nine large research-intensive universities. The interviews,…

Urban Climate Change Resilience as a Teaching Tool for a STEM Summer Bridge Program

NASA Astrophysics Data System (ADS)

Rosenzweig, B.; Vorosmarty, C. J.; Socha, A.; Corsi, F.

2015-12-01

Community colleges have been identified as important gateways for the United States' scientific workforce development. However, students who begin their higher education at community colleges often face barriers to developing the skills needed for higher-level STEM careers, including basic training in mathematics, programming, analytical problem solving, and cross-disciplinary communication. As part of the Business Higher Education Forum's Undergraduate STEM Interventions in Industry (USI2) Consortium, we are developing a summer bridge program for students in STEM fields transferring from community college to senior (4-year) colleges at the City University of New York. Our scientific research on New York City climate change resilience will serve as the foundation for the bridge program curriculum. Students will be introduced to systems thinking and improve their analytical skills through guided problem-solving exercises using the New York City Climate Change Resilience Indicators Database currently being developed by the CUNY Environmental Crossroads Initiative. Students will also be supported in conducting an introductory, independent research project using the database. The interdisciplinary nature of climate change resilience assessment will allow students to explore topics related to their STEM field of interest (i.e. engineering, chemistry, and health science), while working collaboratively across disciplines with their peers. We hope that students that participate in the bridge program will continue with their research projects through their tenure at senior colleges, further enhancing their academic training, while actively contributing to the study of urban climate change resilience. The effectiveness of this approach will be independently evaluated by NORC at the University of Chicago, as well as through internal surveying and long-term tracking of participating student cohorts.

Fe3O4/BSA particles induce osteogenic differentiation of mesenchymal stem cells under static magnetic field.

PubMed

Jiang, Pengfei; Zhang, Yixian; Zhu, Chaonan; Zhang, Wenjing; Mao, Zhengwei; Gao, Changyou

2016-12-01

Differentiation of stem cells is influenced by many factors, yet uptake of the magnetic particles with or without magnetic field is rarely tackled. In this study, iron oxide nanoparticles-loaded bovine serum albumin (BSA) (Fe 3 O 4 /BSA) particles were prepared, which showed a spherical morphology with a diameter below 200 nm, negatively charged surface, and tunable magnetic property. The particles could be internalized into bone marrow mesenchymal stem cells (MSCs), and their release from the cells was significantly retarded under external magnetic field, resulting in almost twice intracellular amount of the particles within 21 d compared to that of the magnetic field free control. Uptake of the Fe 3 O 4 /BSA particles enhanced significantly the osteogenic differentiation of MSCs under a static magnetic field, as evidenced by elevated alkaline phosphatase (ALP) activity, calcium deposition, and expressions of collagen type I and osteocalcin at both mRNA and protein levels. Therefore, uptake of the Fe 3 O 4 /BSA particles brings significant influence on the differentiation of MSCs under magnetic field, and thereby should be paid great attention for practical applications. Differentiation of stem cells is influenced by many factors, yet uptake of the magnetic particles with or without magnetic field is rarely tackled. In this study, iron oxide nanoparticles-loaded bovine serum albumin (BSA) (Fe 3 O 4 /BSA) particles with a diameter below 200nm, negatively charged surface, tunable Fe 3 O 4 content and subsequently adjustable magnetic property were prepared. The particles could be internalized into bone marrow mesenchymal stem cells (MSCs), and their release from the cells was significantly retarded under external magnetic field. Uptake of the Fe 3 O 4 /BSA particles enhanced significantly the osteogenic differentiation of MSCs under a constant static magnetic field, while the magnetic particles and external magnetic field alone do not influence significantly the osteogenic differentiation potential of MSCs regardless of the uptake amount. The results demonstrate a potential magnetic manipulation method for stem cell differentiation, and also convey the significance of careful evaluation of the safety issue of magnetic particles in real an application situation. Copyright © 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Stem Cells: Taking a Closer Look at the Advancements and Hurdles of Stem Cell Research in Australia

ERIC Educational Resources Information Center

Sanderson, Aimee

2008-01-01

The technology surrounding stem cells generates great excitement amongst scientists, media and the community. For science teachers, this means not only embracing and keeping track of the rapid growth and ongoing development in this field but also tackling the ethical and legislative issues surrounding the topic. So what are stem cells, what is all…

High School STEM Teachers' Perceptions of the Work Environment

ERIC Educational Resources Information Center

Pedersen, Daphne E.; West, Robert R.

2017-01-01

How do secondary STEM teachers perceive the environments in which they teach? To what degree is STEM teaching at the secondary level situated in a gendered workplace organization? Using data from the 1999-2000 Schools and Staffing Survey, we examined how men and women who were full-time secondary school teachers in STEM fields (N = 5,617)…

Filling the Gap: Integrating STEM into Career and Technical Education Middle School Programs

ERIC Educational Resources Information Center

Wu-Rorrer, Ray

2017-01-01

The field of STEM education is an educational framework that has surged in application over the past decade. Science, Technology, Engineering, and Math (STEM) is infused in nearly every facet of our society. Filling the gap of current research in middle school career and technical education (CTE) and STEM programs is important as traditional CTE…

Race and Gender Differences in How Sense of Belonging Influences Decisions to Major in STEM

ERIC Educational Resources Information Center

Rainey, Katherine; Dancy, Melissa; Mickelson, Roslyn; Stearns, Elizabeth; Moller, Stephanie

2018-01-01

Background: Women and students of color are widely underrepresented in most STEM fields. In order to investigate this underrepresentation, we interviewed 201 college seniors, primarily women and people of color, who either majored in STEM or started but dropped a STEM major. Here we discuss one section of the longer interview that focused on…

Course-Taking Patterns of Community College Students Beginning in STEM: Using Data Mining Techniques to Reveal Viable STEM Transfer Pathways

ERIC Educational Resources Information Center

Wang, Xueli

2016-01-01

This research focuses on course-taking patterns of beginning community college students enrolled in one or more non-remedial science, technology, engineering, and mathematics (STEM) courses during their first year of college, and how these patterns are mapped against upward transfer in STEM fields of study. Drawing upon postsecondary transcript…

Predicting 9th Graders' Science Self-Efficacy and STEM Career Intent: A Multilevel Approach

ERIC Educational Resources Information Center

Wagstaff, Iris R.

2014-01-01

This study was conducted in response to the growing concern about the lack of U.S. students majoring in STEM fields and pursuing STEM careers (NSF, 2013). In order for the U.S. to compete in a global economy that is increasingly technologically-based, a skilled STEM workforce is a necessity (National Academies, 2010). Understanding the factors…

Conference to focus on solutions to the jobs crisis in STEM fields

NASA Astrophysics Data System (ADS)

Asher, Pranoti

2012-06-01

The inaugural “U.S. News STEM Solutions 2012: A Leadership Summit,” which will take place 27-29 June in Dallas, Tex., will bring together hundreds of leaders in business, education, and government to develop solutions to the jobs crisis in the science, technology, engineering, and math (STEM) fields. With unemployment rates high at the same time that many STEM jobs are going unfilled, the conference will focus on what is working now and what is needed to develop successful local, state, and national action plans to accelerate the development of the STEM workforce in the United States. A broad array of STEM workforce issues will be examined, including how to engage young students and how technology can better align educators with job creators and the skill sets that are required. Summit organizers hope that a key result of the conference will be a national consensus on best practices and the steps needed—in both the short and long term—to ensure a competitive workforce. Another summit objective is to find effective ways to increase public awareness of STEM and its connection to jobs.

Biophysics and dynamics of natural and engineered stem cell microenvironments.

PubMed

Keung, Albert J; Healy, Kevin E; Kumar, Sanjay; Schaffer, David V

2010-01-01

Stem cells are defined by their ability to self-renew and to differentiate into one or more mature lineages, and they reside within natural niches in many types of adult and embryonic tissues that present them with complex signals to regulate these two hallmark properties. The diverse nature of these in vivo microenvironments raises important questions about the microenvironmental cues regulating stem cell plasticity, and the stem cell field has built a strong foundation of knowledge on the biochemical identities and regulatory effects of the soluble, cellular, and extracellular matrix factors surrounding stem cells through the isolation and culture of stem cells in vitro within microenvironments that, in effect, emulate the properties of the natural niche. Recent work, however, has expanded the field's perspective to include biophysical and dynamic characteristics of the microenvironment. These include biomechanical characteristics such as elastic modulus, shear force, and cyclic strain; architectural properties such as geometry, topography, and dimensionality; and dynamic structures and ligand profiles. We will review how these microenvironmental characteristics have been shown to regulate stem cell fate and discuss future research directions that may help expand our current understanding of stem cell biology and aid its application to regenerative medicine.

Concise Review: Stem Cell Population Biology: Insights from Hematopoiesis.

PubMed

MacLean, Adam L; Lo Celso, Cristina; Stumpf, Michael P H

2017-01-01

Stem cells are fundamental to human life and offer great therapeutic potential, yet their biology remains incompletely-or in cases even poorly-understood. The field of stem cell biology has grown substantially in recent years due to a combination of experimental and theoretical contributions: the experimental branch of this work provides data in an ever-increasing number of dimensions, while the theoretical branch seeks to determine suitable models of the fundamental stem cell processes that these data describe. The application of population dynamics to biology is amongst the oldest applications of mathematics to biology, and the population dynamics perspective continues to offer much today. Here we describe the impact that such a perspective has made in the field of stem cell biology. Using hematopoietic stem cells as our model system, we discuss the approaches that have been used to study their key properties, such as capacity for self-renewal, differentiation, and cell fate lineage choice. We will also discuss the relevance of population dynamics in models of stem cells and cancer, where competition naturally emerges as an influential factor on the temporal evolution of cell populations. Stem Cells 2017;35:80-88. © 2016 AlphaMed Press.

Technology advancement for integrative stem cell analyses.

PubMed

Jeong, Yoon; Choi, Jonghoon; Lee, Kwan Hyi

2014-12-01

Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose--by introducing a concept of vertical and horizontal approach--that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment.

Technology Advancement for Integrative Stem Cell Analyses

PubMed Central

Jeong, Yoon

2014-01-01

Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose—by introducing a concept of vertical and horizontal approach—that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment. PMID:24874188

The educational journeys of first-generation college women in STEM: A grounded theory study

NASA Astrophysics Data System (ADS)

Geier, Susan

The purpose of this study was to ascertain the various factors that influenced these first-generation college women as they chose a college and selected a STEM major and subsequently persisted to upper level (junior/senior) status. Twenty-five first-generation college women in STEM majors who attended a research-intensive university in the Midwest were interviewed. Approaching this study using constructivist grounded theory provided the opportunity for deeper insights by examining data at a conceptual level while preserving the voices of the women in this study. The women faced numerous challenges on their journeys, yet they persisted. As the women in this study selected and persisted in STEM, they demonstrated thoughtful determination, experienced shifting identities, established purposeful relationships and applied forward thinking, as they practiced high-stakes decision-making during their journeys. The experiences of these women, namely first-generation women in STEM fields, may inform students, parents, educators, researchers, and policymakers concerned with (a) inspiring students to consider STEM majors, (b) fostering student success in STEM throughout their academic journeys, and (c) ultimately increasing the number of underrepresented minorities and women in the STEM fields.

Biomaterials and Stem Cells for Tissue Engineering

PubMed Central

Zhang, Zhanpeng; Gupte, Melanie J.; Ma, Peter X.

2013-01-01

Importance of the field Organ failure and tissue loss are challenging health issues due to widespread injury, the lack of organs for transplantation, and limitations of conventional artificial implants. The field of tissue engineering aims to provide alternative living substitutes that restore, maintain or improve tissue function. Areas covered in this review In this paper, a wide range of porous scaffolds are reviewed, with an emphasis on phase separation techniques that generate advantageous nanofibrous 3D scaffolds for stem cell-based tissue engineering applications. In addition, methods for presentation and delivery of bioactive molecules to mimic the properties of stem cell niche are summarized. Recent progress in using these bio-instructive scaffolds to support stem cell differentiation and tissue regeneration is also presented. What the reader will gain Stem cells have great clinical potential because of their capability to differentiate into multiple cell types. Biomaterials have served as artificial extracellular environments to regulate stem cell behavior. Biomaterials with various physical, mechanical, and chemical properties can be designed to control stem cell development for regeneration. Take home message The research at the interface of stem cell biology and biomaterials has made and will continue to make exciting advances in tissue engineering. PMID:23327471

Upward Transfer in STEM Fields of Study: A New Conceptual Framework and Survey Instrument for Institutional Research

ERIC Educational Resources Information Center

Wang, Xueli

2016-01-01

This chapter describes a new conceptual framework that informs research on factors influencing transfer in STEM fields of study from 2-year to 4-year institutions, presents a new survey instrument based on the framework, and offers directions for future research in this area.

The Underrepresentation of African American Female Students in STEM Fields: Implications for Classroom Teachers

ERIC Educational Resources Information Center

Farinde, Abiola A.; Lewis, Chance W.

2012-01-01

African American women are underrepresented in STEM (science, technology, engineering and math) fields (Catsambis, 1994). The socialization and "under-education" of African American female students engenders ideas of inferiority, while the presence of an inferior race, sex and class, in one body, may produce an ideology of mediocrity.…

To Stay or Not to Stay: Retention of Asian International Faculty in STEM Fields

ERIC Educational Resources Information Center

Lawrence, Janet H.; Celis, Sergio; Kim, Hee Sun; Lipson, Sarah Ketchen; Tong, Ximeng

2014-01-01

The present study identifies characteristics of individuals and work settings that influence Asian international faculty members' intentions to continue their employment in US research universities. Given the demand for researchers in science, technology, engineering and mathematics fields (STEM), the higher rate of turnover among untenured…

The Effect of Learning Communities on Achievement in STEM Fields for African Americans across Four Campuses

ERIC Educational Resources Information Center

Taylor, Orlando L.; McGowan, Jill; Alston, Sharon T.

2008-01-01

Four historically Black colleges and universities (HBCUs), Howard University, Jackson State University, Talladega College, and Xavier University of Louisiana, participated in a project titled, Learning Communities for STEM Academic Achievement (LCSAA), whose goal was to increase the participation of African American students in the fields of…

The C-MORE Scholars Program: Engaging minority students in STEM through undergraduate research

NASA Astrophysics Data System (ADS)

Gibson, B. A.; Bruno, B. C.

2010-12-01

There have been several studies that show how undergraduate research experiences (REU) have a positive impact on a student’s academic studies and career path, including being a positive influence toward improving the student's lab skills and ability to work independently. Moreover, minority students appear to relate to science, technology, engineering, and mathematics (STEM) concepts better when they are linked with (1) a service learning component, and (2) STEM courses that include a cultural and social aspect that engages the student in a way that does not distract from the student’s technical learning. It is also known that a “place-based” approach that incorporates traditional (indigenous) knowledge can help engage underrepresented minority groups in STEM disciplines and increase science literacy. Based on the methods and best practices used by other minority serving programs and described in the literature, the Center for Microbial Oceanography: Research and Education (C-MORE) has successfully developed an academic-year REU to engage and train the next generation of scientists. The C-MORE Scholars Program provides undergraduate students majoring in an ocean or earth science-related field, especially underrepresented students such as Native Hawaiians and Pacific Islanders, the opportunity to participate in unique and cutting edge hands-on research experiences. The program appoints awardees at one of three levels based on previous research and academic experience, and students can progress through the various tiers as their skills and STEM content knowledge develop. All awardees receive guidance on a research project from a mentor who is a scientist at the university and/or industry. A key component of the program is the inclusion of professional development activities to help the student continue towards post graduation education or prepare for career opportunities after they receive their undergraduate STEM degree.

Whole-cell imaging of the budding yeast Saccharomyces cerevisiae by high-voltage scanning transmission electron tomography.

PubMed

Murata, Kazuyoshi; Esaki, Masatoshi; Ogura, Teru; Arai, Shigeo; Yamamoto, Yuta; Tanaka, Nobuo

2014-11-01

Electron tomography using a high-voltage electron microscope (HVEM) provides three-dimensional information about cellular components in sections thicker than 1 μm, although in bright-field mode image degradation caused by multiple inelastic scattering of transmitted electrons limit the attainable resolution. Scanning transmission electron microscopy (STEM) is believed to give enhanced contrast and resolution compared to conventional transmission electron microscopy (CTEM). Samples up to 1 μm in thickness have been analyzed with an intermediate-voltage electron microscope because inelastic scattering is not a critical limitation, and probe broadening can be minimized. Here, we employed STEM at 1 MeV high-voltage to extend the useful specimen thickness for electron tomography, which we demonstrate by a seamless tomographic reconstruction of a whole, budding Saccharomyces cerevisiae yeast cell, which is ~3 μm in thickness. High-voltage STEM tomography, especially in the bright-field mode, demonstrated sufficiently enhanced contrast and intensity, compared to CTEM tomography, to permit segmentation of major organelles in the whole cell. STEM imaging also reduced specimen shrinkage during tilt-series acquisition. The fidelity of structural preservation was limited by cytoplasmic extraction, and the spatial resolution was limited by the relatively large convergence angle of the scanning probe. However, the new technique has potential to solve longstanding problems of image blurring in biological specimens beyond 1 μm in thickness, and may facilitate new research in cellular structural biology. Copyright © 2014 Elsevier B.V. All rights reserved.

Programming experience promotes higher STEM motivation among first-grade girls.

PubMed

Master, Allison; Cheryan, Sapna; Moscatelli, Adriana; Meltzoff, Andrew N

2017-08-01

The gender gap in science, technology, engineering, and math (STEM) engagement is large and persistent. This gap is significantly larger in technological fields such as computer science and engineering than in math and science. Gender gaps begin early; young girls report less interest and self-efficacy in technology compared with boys in elementary school. In the current study (N=96), we assessed 6-year-old children's stereotypes about STEM fields and tested an intervention to develop girls' STEM motivation despite these stereotypes. First-grade children held stereotypes that boys were better than girls at robotics and programming but did not hold these stereotypes about math and science. Girls with stronger stereotypes about robotics and programming reported lower interest and self-efficacy in these domains. We experimentally tested whether positive experience with programming robots would lead to greater interest and self-efficacy among girls despite these stereotypes. Children were randomly assigned either to a treatment group that was given experience in programming a robot using a smartphone or to control groups (no activity or other activity). Girls given programming experience reported higher technology interest and self-efficacy compared with girls without this experience and did not exhibit a significant gender gap relative to boys' interest and self-efficacy. These findings show that children's views mirror current American cultural messages about who excels at computer science and engineering and show the benefit of providing young girls with chances to experience technological activities. Copyright © 2017 Elsevier Inc. All rights reserved.

Young Women's Conference in STEM: Our starting point for getting women into STEM fields

NASA Astrophysics Data System (ADS)

Ortiz, Deedee; Zwicker, Andrew; Greco, Shannon; Dominguez, Arturo

2015-11-01

The number of women in STEM careers is staggeringly low; just one in seven engineers are female and only 27% of all computer science jobs are held by women. A recent US Dept. of Commerce report found that between 2000-2011 women experienced no employment growth in STEM jobs. According to the AIP, the percentage of women in physics overall is 26% and women make up less than 7% of the fusion energy workforce. To address this problem we have, since 2001, run a conference to introduce young women to the wide range of careers in STEM fields in a way that is not part of their typical education. By introducing students in a meaningful way to successful women in STEM, from graduate students to senior researchers, the intent of the conference is to foster interest, develop mentoring relationships, and to provide role models that will have a positive influence on future educational and career choices. Data from surveys indicate that this is indeed the impact. For example, 86% of 2015 attendees indicated they are more likely to major in a scientific field after attending the conference then they were before. We are now in the process of expanding and improving the conference in order to reach more students and increase the overall impact.

Gender and family influences on Spanish students' aspirations and values in stem fields

NASA Astrophysics Data System (ADS)

Sáinz, Milagros; Müller, Jörg

2018-01-01

Drawing on expectancy-value theory, this study examines gender and family influences on students' career aspirations and attached values. 796 secondary Spanish students (M age = 16 years old, S.D. = 0.81) participated. 53% were boys. The results show that boys and students with mothers who have completed intermediate level education were more interested in science, technology, engineering and mathematics (STEM) architecture and technology. Girls and students with highly educated mothers born in Spain were more likely to aspire to STEM health and experimental studies. Furthermore, boys and students planning to pursue STEM-technology studies attached higher extrinsic values to these studies. On the contrary, girls and participants with interest in experimental and health studies attached less extrinsic values to these studies. Moreover, students with highly educated mothers and interested in STEM architecture and technology reported higher extrinsic values. Understanding the interaction of gender and family factors shaping adolescents' career aspirations in STEM fields seems to be crucial to designing significant and effective school and family grounded interventions.

Biogeographical patterns of biomass allocation in leaves, stems, and roots in China's forests.

PubMed

Zhang, Hao; Wang, Kelin; Xu, Xianli; Song, Tongqing; Xu, Yanfang; Zeng, Fuping

2015-11-03

To test whether there are general patterns in biomass partitioning in relation to environmental variation when stand biomass is considered, we investigated biomass allocation in leaves, stems, and roots in China's forests using both the national forest inventory data (2004-2008) and our field measurements (2011-2012). Distribution patterns of leaf, stem, and root biomass showed significantly different trends according to latitude, longitude, and altitude, and were positively and significantly correlated with stand age and mean annual precipitation. Trade-offs among leaves, stems, and roots varied with forest type and origin and were mainly explained by stand biomass. Based on the constraints of stand biomass, biomass allocation was also influenced by forest type, origin, stand age, stand density, mean annual temperature, precipitation, and maximum temperature in the growing season. Therefore, after stand biomass was accounted for, the residual variation in biomass allocation could be partially explained by stand characteristics and environmental factors, which may aid in quantifying carbon cycling in forest ecosystems and assessing the impacts of climate change on forest carbon dynamics in China.

Biogeographical patterns of biomass allocation in leaves, stems, and roots in China’s forests

PubMed Central

Zhang, Hao; Wang, Kelin; Xu, Xianli; Song, Tongqing; Xu, Yanfang; Zeng, Fuping

2015-01-01

To test whether there are general patterns in biomass partitioning in relation to environmental variation when stand biomass is considered, we investigated biomass allocation in leaves, stems, and roots in China’s forests using both the national forest inventory data (2004–2008) and our field measurements (2011–2012). Distribution patterns of leaf, stem, and root biomass showed significantly different trends according to latitude, longitude, and altitude, and were positively and significantly correlated with stand age and mean annual precipitation. Trade-offs among leaves, stems, and roots varied with forest type and origin and were mainly explained by stand biomass. Based on the constraints of stand biomass, biomass allocation was also influenced by forest type, origin, stand age, stand density, mean annual temperature, precipitation, and maximum temperature in the growing season. Therefore, after stand biomass was accounted for, the residual variation in biomass allocation could be partially explained by stand characteristics and environmental factors, which may aid in quantifying carbon cycling in forest ecosystems and assessing the impacts of climate change on forest carbon dynamics in China. PMID:26525117

Hydraulic Function in Australian Tree Species during Drought-Induced Mortality

NASA Astrophysics Data System (ADS)

Tissue, D.; Maier, C.; Creek, D.; Choat, B.

2016-12-01

Drought induced tree mortality and decline are key issues facing forest ecology and management. Here, we primarily investigated the hydraulic limitations underpinning drought-induced mortality in three Australian tree species. Using field-based large rainout shelters, three angiosperm species (Casuarina cunninghamiana, Eucalyptus sideroxylon, Eucalyptus tereticornis) were subjected to two successive drought and recovery cycles, prior to a subsequent long and extreme drought to mortality; total duration of experiment was 2.5 years. Leaf gas exchange, leaf and stem hydraulics, and carbon reserves were monitored during the experiment. Trees died as a result of failure in the hydraulic transport system, primarily related to water stress induced embolism. Stomatal closure occurred prior to the induction of significant embolism in the stem xylem of all species. Nonetheless, trees suffered a rapid decline in xylem water potential and increase in embolism during the severe drought treatment. Trees died at water potentials causing greater than 90% loss of hydraulic conductivity in the stem, providing support for the theory that lethal water potential is correlated with complete loss of hydraulic function in the stem xylem of angiosperms.

Turning the STEM Tide: An Approach for Mentoring Young Women on How to Thrive in STEM Careers

DTIC Science & Technology

2014-08-01

facilitating candid interactive discussions on trends, professional and personal life balance , and obstacles and opportunities for women in STEM fields...access to successful women in STEM with candid discussions focused on women and STEM careers, achieving balance between profession and personal life ...doctorate degrees were 11% less likely than single women to work in science. Another study by Mason and Goulden (2002) showed that the effect of

Can Financial Aid Help to Address the Growing Need for STEM Education? The Effects of Need-Based Grants on the Completion of Science, Technology, Engineering, and Math Courses and Degrees

ERIC Educational Resources Information Center

Castleman, Benjamin L.; Long, Bridget Terry; Mabel, Zachary

2018-01-01

Although workers in science, technology, engineering, and math (STEM) fields earn above-average wages, the number of college graduates prepared for STEM jobs lags behind employer demand. A key question is how to recruit and retain college students in STEM majors. We offer new evidence on the role of financial aid in supporting STEM attainment.…

Therapeutic Potential, Challenges and Future Perspective of Cancer Stem Cells in Translational Oncology: A Critical Review.

PubMed

Shukla, Gaurav; Khera, Harvinder Kour; Srivastava, Amit Kumar; Khare, Piush; Patidar, Rahul; Saxena, Rajiv

2017-01-01

Stem cell research is a rapidly developing field that offers effective treatment for a variety of malignant and non-malignant diseases. Stem cell is a regenerative medicine associated with the replacement, repair, and restoration of injured tissue. Stem cell research is a promising field having maximum therapeutic potential. Cancer stem cells (CSCs) are the cells within the tumor that posses capacity of selfrenewal and have a root cause for the failure of traditional therapies leading to re-occurrence of cancer. CSCs have been identified in blood, breast, brain, and colon cancer. Traditional therapies target only fast growing tumor mass, but not slow-dividing cancer stem cells. It has been shown that embryonic pathways such as Wnt, Hedgehog and Notch, control self-renewal capacity and involved in cancer stem cell maintenance. Targeting of these pathways may be effective in eradicating cancer stem cells and preventing chemotherapy and radiotherapy resistance. Targeting CSCs has become one of the most effective approaches to improve the cancer survival by eradicating the main root cause of cancer. The present review will address, in brief, the importance of cancer stem cells in targeting cancer as better and effective treatment along with a concluding outlook on the scope and challenges in the implication of cancer stem cells in translational oncology. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Experiences of African American Young Women in Science, Technology, Engineering, and Mathematics (STEM) Education

NASA Astrophysics Data System (ADS)

Kolo, Yovonda Ingram

African American women are underrepresented in science, technology, engineering, and mathematics (STEM) fields throughout the United States. As the need for STEM professionals in the United States increases, it is important to ensure that African American women are among those professionals making valuable contributions to society. The purpose of this phenomenological study was to describe the experiences of African American young women in relation to STEM education. The research question for this study examined how experiences with STEM in K-10 education influenced African American young women's academic choices in their final years in high school. The theory of multicontextuality was used to provide the conceptual framework. The primary data source was interviews. The sample was composed of 11 African American young women in their junior or senior year in high school. Data were analyzed through the process of open coding, categorizing, and identifying emerging themes. Ten themes emerged from the answers to research questions. The themes were (a) high teacher expectations, (b) participation in extra-curricular activities, (c) engagement in group-work, (d) learning from lectures, (e) strong parental involvement, (f) helping others, (g) self-efficacy, (h) gender empowerment, (i) race empowerment, and (j) strategic recruitment practices. This study may lead to positive social change by adding to the understanding of the experiences of African American young women in STEM. By doing so, these findings might motivate other African American young women to pursue advanced STEM classes. These findings may also provide guidance to parents and educators to help increase the number of African American women in STEM.

Indicators of science, technology, engineering, and math (STEM) career interest among middle school students in the USA

NASA Astrophysics Data System (ADS)

Mills, Leila A.

This study examines middle school students' perceptions of a future career in a science, math, engineering, or technology (STEM) career field. Gender, grade, predispositions to STEM contents, and learner dispositions are examined for changing perceptions and development in career-related choice behavior. Student perceptions as measured by validated measurement instruments are analyzed pre and post participation in a STEM intervention energy-monitoring program that was offered in several U.S. middle schools during the 2009-2010, 2010-2011 school years. A multiple linear regression (MLR) model, developed by incorporating predictors identified by an examination of the literature and a hypothesis-generating pilot study for prediction of STEM career interest, is introduced. Theories on the career choice development process from authors such as Ginzberg, Eccles, and Lent are examined as the basis for recognition of career concept development among students. Multiple linear regression statistics, correlation analysis, and analyses of means are used to examine student data from two separate program years. Study research questions focus on predictive ability, RSQ, of MLR models by gender/grade, and significance of model predictors in order to determine the most significant predictors of STEM career interest, and changes in students' perceptions pre and post program participation. Analysis revealed increases in the perceptions of a science career, decreases in perceptions of a STEM career, increase of the significance of science and mathematics to predictive models, and significant increases in students' perceptions of creative tendencies.

Improving estimation of tree carbon stocks by harvesting aboveground woody biomass within airborne LiDAR flight areas

NASA Astrophysics Data System (ADS)

Colgan, M.; Asner, G. P.; Swemmer, A. M.

2011-12-01

The accurate estimation of carbon stored in a tree is essential to accounting for the carbon emissions due to deforestation and degradation. Airborne LiDAR (Light Detection and Ranging) has been successful in estimating aboveground carbon density (ACD) by correlating airborne metrics, such as canopy height, to field-estimated biomass. This latter step is reliant on field allometry which is applied to forest inventory quantities, such as stem diameter and height, to predict the biomass of a given tree stem. Constructing such allometry is expensive, time consuming, and requires destructive sampling. Consequently, the sample sizes used to construct such allometry are often small, and the largest tree sampled is often much smaller than the largest in the forest population. The uncertainty resulting from these sampling errors can lead to severe biases when the allometry is applied to stems larger than those harvested to construct the allometry, which is then subsequently propagated to airborne ACD estimates. The Kruger National Park (KNP) mission of maintaining biodiversity coincides with preserving ecosystem carbon stocks. However, one hurdle to accurately quantifying carbon density in savannas is that small stems are typically harvested to construct woody biomass allometry, yet they are not representative of Kruger's distribution of biomass. Consequently, these equations inadequately capture large tree variation in sapwood/hardwood composition, root/shoot/leaf allocation, branch fall, and stem rot. This study eliminates the "middleman" of field allometry by directly measuring, or harvesting, tree biomass within the extent of airborne LiDAR. This enables comparisons of field and airborne ACD estimates, and also enables creation of new airborne algorithms to estimate biomass at the scale of individual trees. A field campaign was conducted at Pompey Silica Mine 5km outside Kruger National Park, South Africa, in Mar-Aug 2010 to harvest and weigh tree mass. Since harvesting of trees is not possible within KNP, this was a unique opportunity to fell trees already scheduled to be cleared for mining operations. The area was first flown by the Carnegie Airborne Observatory in early May, prior to harvest, to enable correlation of LiDAR-measured tree height and crown diameter to harvested tree mass. Results include over 4,000 harvested stems and 13 species-specific biomass equations, including seven Kruger woody species previously without allometry. We found existing biomass stem allometry over-estimates ACD in the field, whereas airborne estimates based on harvest data avoid this bias while maintaining similar precision to field-based estimates. Lastly, a new airborne algorithm estimating biomass at the tree-level reduced error from tree canopies "leaning" into field plots but whose stems are outside plot boundaries. These advances pave the way to better understanding of savanna and forest carbon density at landscape and regional scales.

The human urothelium consists of multiple clonal units, each maintained by a stem cell.

PubMed

Gaisa, Nadine T; Graham, Trevor A; McDonald, Stuart A C; Cañadillas-Lopez, Sagrario; Poulsom, Richard; Heidenreich, Axel; Jakse, Gerhard; Tadrous, Paul J; Knuechel, Ruth; Wright, Nicholas A

2011-10-01

Little is known about the clonal architecture of human urothelium. It is likely that urothelial stem cells reside within the basal epithelial layer, yet lineage tracing from a single stem cell as a means to show the presence of a urothelial stem cell has never been performed. Here, we identify clonally related cell areas within human bladder mucosa in order to visualize epithelial fields maintained by a single founder/stem cell. Sixteen frozen cystectomy specimens were serially sectioned. Patches of cells deficient for the mitochondrially encoded enzyme cytochrome c oxidase (CCO) were identified using dual-colour enzyme histochemistry. To show that these patches represent clonal proliferations, small CCO-proficient and -deficient areas were individually laser-capture microdissected and the entire mitochondrial genome (mtDNA) in each area was PCR amplified and sequenced to identify mtDNA mutations. Immunohistochemistry was performed for the different cell layers of the urothelium and adjacent mesenchyme. CCO-deficient patches could be observed in normal urothelium of all cystectomy specimens. The two-dimensional length of these negative patches varied from 2-3 cells (about 30 µm) to 4.7 mm. Each cell area within a CCO-deficient patch contained an identical somatic mtDNA mutation, indicating that the patch was a clonal unit. Patches contained all the mature cell differentiation stages present in the urothelium, suggesting the presence of a stem cell. Our results demonstrate that the normal mucosa of human bladder contains stem cell-derived clonal units that actively replenish the urothelium during ageing. The size of the clonal unit attributable to each stem cell was broadly distributed, suggesting replacement of one stem cell clone by another. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

A miniature ultrasonic actuator-control system for plant stem diameter micro-variation measurements

USDA-ARS?s Scientific Manuscript database

Measurements of micro-variations in plant stem diameter are potentially useful to optimize irrigation decision support systems that are based on plant physiological responses. However, for this technology to be suitable for field applications, problems associated with stem softness and micro variati...

Shifting Expectations: Bringing STEM to Scale through Expanded Learning Systems

ERIC Educational Resources Information Center

Donner, Jessica; Wang, Yvonne

2013-01-01

Expanded learning opportunities, such as afterschool and summer programs, are particularly well positioned to help address science, technology, engineering, and mathematics (STEM) education crisis. A large percentage of youth participating in afterschool programs are members of groups traditionally underrepresented in STEM fields. Additionally,…

Diversifying the STEM Pipeline: The Model Replication Institutions Program

ERIC Educational Resources Information Center

Cullinane, Jenna

2009-01-01

In 2006, the National Science Foundation (NSF) began funding the Model Replication Institutions (MRI) program, which sought to improve the quality, availability, and diversity of science, technology, engineering, and mathematics (STEM) education. Faced with pressing national priorities in the STEM fields and chronic gaps in postsecondary…

Ethical clinical translation of stem cell interventions for neurologic disease.

PubMed

Cote, David J; Bredenoord, Annelien L; Smith, Timothy R; Ammirati, Mario; Brennum, Jannick; Mendez, Ivar; Ammar, Ahmed S; Balak, Naci; Bolles, Gene; Esene, Ignatius Ngene; Mathiesen, Tiit; Broekman, Marike L

2017-01-17

The application of stem cell transplants in clinical practice has increased in frequency in recent years. Many of the stem cell transplants in neurologic diseases, including stroke, Parkinson disease, spinal cord injury, and demyelinating diseases, are unproven-they have not been tested in prospective, controlled clinical trials and have not become accepted therapies. Stem cell transplant procedures currently being carried out have therapeutic aims, but are frequently experimental and unregulated, and could potentially put patients at risk. In some cases, patients undergoing such operations are not included in a clinical trial, and do not provide genuinely informed consent. For these reasons and others, some current stem cell interventions for neurologic diseases are ethically dubious and could jeopardize progress in the field. We provide discussion points for the evaluation of new stem cell interventions for neurologic disease, based primarily on the new Guidelines for Stem Cell Research and Clinical Translation released by the International Society for Stem Cell Research in May 2016. Important considerations in the ethical translation of stem cells to clinical practice include regulatory oversight, conflicts of interest, data sharing, the nature of investigation (e.g., within vs outside of a clinical trial), informed consent, risk-benefit ratios, the therapeutic misconception, and patient vulnerability. To help guide the translation of stem cells from the laboratory into the neurosurgical clinic in an ethically sound manner, we present an ethical discussion of these major issues at stake in the field of stem cell clinical research for neurologic disease. © 2016 American Academy of Neurology.

Measuring post-secondary stem majors' engagement in sustainability: The creation, assessment, and validation of an instrument for sustainability curricula evaluation

NASA Astrophysics Data System (ADS)

Little, David L., II

Ongoing changes in values, pedagogy, and curriculum concerning sustainability education necessitate that strong curricular elements are identified in sustainability education. However, quantitative research in sustainability education is largely undeveloped or relies on outdated instruments. In part, this is because no widespread quantitative instrument for measuring related educational outcomes has been developed for the field, though their development is pivotal for future efforts in sustainability education related to STEM majors. This research study details the creation, evaluation, and validation of an instrument -- the STEM Sustainability Engagement Instrument (STEMSEI) -- designed to measure sustainability engagement in post-secondary STEM majors. The study was conducted in three phases, using qualitative methods in phase 1, a concurrent mixed methods design in phase 2, and a sequential mixed methods design in phase 3. The STEMSEI was able to successfully predict statistically significant differences in the sample (n= 1017) that were predicted by prior research in environmental education. The STEMSEI also revealed statistically significant differences between STEM majors' sustainability engagement with a large effect size (.203 ≤ eta2 ≤ .211). As hypothesized, statistically significant differences were found on the environmental scales across gender and present religion. With respect to gender, self-perceived measures of emotional engagement with environmental sustainability was higher with females while males had higher measures in cognitive engagement with respect to knowing information related to environmental sustainability. With respect to present religion, self-perceived measures of general engagement and emotional engagement in environmental sustainability were higher for non-Christians as compared to Christians. On the economic scales, statistically significant differences were found across gender. Specifically, measures of males' self-perceived cognitive engagement in knowing information related to economic sustainability were greater than those of females. Future research should establish the generalizability of these results and further test the validity of the STEMSEI.

Virulence of Hymenoscyphus albidus and H. fraxineus on Fraxinus excelsior and F. pennsylvanica

PubMed Central

Kowalski, Tadeusz; Bilański, Piotr; Holdenrieder, Ottmar

2015-01-01

European ash (Fraxinus excelsior) is currently battling an onslaught of ash dieback, a disease emerging in the greater part of its native area, brought about by the introduction of the ascomycete Hymenoscyphus fraxineus (= Hymenoscyphus pseudoalbidus). The closely-related fungus Hymenoscyphus albidus, which is indigenous to Europe, is non-pathogenic when in contact with F. excelsior, but could pose a potential risk to exotic Fraxinus species. The North American green ash (Fraxinus pennsylvanica) is planted widely throughout Europe and regenerates naturally within this environment but little is known about the susceptibility of this species to ash dieback. We performed wound inoculations with both fungi (nine strains of H. fraxineus and three strains of H. albidus) on rachises and stems of F. excelsior and F. pennsylvanica under field conditions in Southern Poland. Necrosis formation was evaluated after two months on the rachises and after 12 months on the stems. After inoculation of H. albidus, only small lesions (of up to 1.3 cm in length) developed on the F. excelsior and F. pennsylvanica rachises, but with no significant distinction from the controls. Hymenoscyphus albidus did not cause necrotic lesions on the stems of either Fraxinus species. In contrast, H. fraxineus induced necroses on all inoculated rachises of both ash species with mean lengths of 8.4 cm (F. excelsior) and 1.9 cm (F. pennsylvanica). Necroses also developed on all of the inoculated F. excelsior stems (mean length 18.0 cm), whereas on F. pennsylvanica such lesions only occurred on about 5% of the stems (mean length 1.9 cm). The differences between strains were negligible. No necroses were observed on the control plants. Reisolations of H. albidus were only successful in around 8–11% of the cases, while H. fraxineus was reisolated from 50–70% of the inoculated organs showing necrotic lesions. None of the Hymenoscyphus species were isolated from the control plants. Our data confirm H. fraxineus’ high virulence with regards to F. excelsior and demonstrate a low virulence in relation to F. pennsylvanica under field conditions in Poland. Hymenoscyphus albidus did not express any perceivable pathogenicity on both host species. PMID:26517266

STEM Education is Missing This.......

NASA Astrophysics Data System (ADS)

Orr, Laura; Johnson, Milton; Miller, Alexandra; Rebull, Luisa M.

2017-01-01

STEM education gets a lot of attention in schools, media, politics, and funding. But while the acronym grows from STEM to STEAM to STREAM, we still see a lack of student participation in real science, using big data and building partnerships with professionals in the field, and real student growth in science achievement. After the NITARP experience, we believe that NITARP is a rich, demanding, and authentic experience for dedicated teachers and students that provides a caliber of learning that is hard, if not impossible, to achieve in the traditional classroom. This poster looks at what STEM still needs to be and become for it to be the driving force behind greater student involvement, interest, and increased academic performance in the sciences. We focus on our own experiences and that of our students; our different teaching backgrounds and school environments; and the effects we see on our students using traditional and new STEM education and participation in the NITARP program. We come from backgrounds and situations that range from urban to rural, middle to high school, wide socioeconomic variety, gender differences, as well as different exposures to STEM opportunities. We propose that traditional and current standards for STEM education are falling short of what is needed for students to truly experience, understand, and gain the skills to accurately apply and advance in science. Incoming and current science teachers at all levels are not provided with quality, realistic, or applicable preparation. NITARP is truly a STEM experience because it actually integrates the 4 fields and provides opportunities for students to experience the overlap of the 4 fields in an authentic way. The deep, long term exposure to authentic research and technology as well as opportunity to talk with working scientists in a variety of fields have a huge impact on the students and teachers alike. Exposure to programs and experiences like NITARP are needed to help drive and support STEM education to meet its goals and intentions. Support provided for this work by the NASA/IPAC Teacher Archive Research Program (NITARP), which receives funding from the NASA ADP program.

The Longitudinal STEM Identity Trajectories of Middle School Girls who Participated in a Single-Sex Informal STEM Education Program

NASA Astrophysics Data System (ADS)

Hughes, Roxanne

2014-03-01

This study examined the longitudinal effects of participation in an all-girls STEM summer camp on young women's interest in STEM fields and motivation to pursue these fields. The SciGirls camp has been in existence since 2006, with its goal of providing a safe space for young women to explore STEM careers and strengthen their interest in these careers. Over 166 middle school age girls have participated in the program since it began in 2006. Of those participants, 60 responded to at least one of the follow up surveys that are sent every three years - 2009 and 2012. The surveys attempt to determine participants' level of interest in STEM. The survey was qualitative in nature and asked open ended questions. Results indicated that the camp had a positive effect on participants' perceptions of scientists and their work. This study adds to the literature that looks at the longitudinal impacts of informal STEM educational programs that expose young women to female scientist role models and mentors. This study supports the research that claims that exposing young women at an early age to science role models can positively alter their perception of science careers which can eventually increase the number of women who pursue these careers. This increase is important at a time when men still outnumber women in many science and engineering fields. This study was funded in part by the National Science Foundation Division of Materials Research through DMR 0654118.

Determination of thiamethoxam residues in banana stem and fruit through LC-MS/MS.

PubMed

Suganthi, A; Nikita, S A; Kousika, J; Bhuvaneswari, K; Sridharan, S

2018-04-18

An analytical method based on liquid chromatography coupled with mass spectroscopy/mass spectroscopy was developed and validated for the determination of thiamethoxam residues in banana fruit and stem tissue samples. In this study, Waters Alliance LC and Acquity TQD were used with an electrospray ionization interface in the positive ion mode. An isocratic flow of 0.5% HCOOH in water and 0.05% HCOOH in CH 3 CN was used for separation. Thiamethoxam residue was extracted from the samples using CH 3 CN and a dispersive solid-phase extraction method was used for subsequent cleanup. Linearity studies were conducted between 0.001 and 0.1 μg mL -1 of standard solution with three replicates for each concentration. Satisfactory recoveries (107.21 to 115.16% and 90.94 to 109.22%) and high precision (relative standard deviations of 3.71 to 12.83% and 3.24 to 10.78%) were obtained for the banana stem and banana fruit matrix, respectively. The lower limits of detection and quantification achieved were 0.002 and 0.008 μg g -1 for banana stem and 0.001and 0.005 μg g -1 for banana fruit, respectively. The developed method was used to analyze the banana stem and fruit samples collected from thiamethoxam-treated fields and stems from the local market.

The Role of Stem Cell Therapeutics in Wound Healing: Current Understanding and Future Directions.

PubMed

Sorice, Sarah; Rustad, Kristine C; Li, Alexander Y; Gurtner, Geoffrey C

2016-09-01

Chronic wounds present unique challenges for healthcare providers as they place patients at increased risk for various morbidities and mortality. Advances in wound care technology have expanded the treatment options available for wound management, but few products fully address the underlying core deficiencies responsible for the development of poorly healing wounds. In the future, addressing these derangements will undoubtedly play a key role in the treatment of these patients. Broad enthusiasm has surrounded the field of stem cell biology, which has shown great promise in repairing damaged tissues across numerous disease phenotypes. In this review, we provide a comprehensive review of the literature and evaluate the present landscape of wound therapeutics while discussing the rationales and allure behind stem cell-based products. We further propose 2 challenges that remain as new stem cell-based therapies are being developed and as this technology moves toward clinical translation. Given the relatively young age of this newer technology in wound healing, numerous challenges continue to surround its effective use including identifying the ideal population of stem cells to use and determining the optimal cell delivery method. However, significant forward progress has been made, with several clinical trials beginning to demonstrate reliable clinical benefit. The upward trajectory of stem cell technologies provides an exciting opportunity to positively impact patient outcomes through the controlled application of regenerative cell-based therapy.

Do rice water weevils and rice stem borers compete when sharing a host plant?*

PubMed Central

Shi, Sheng-wei; He, Yan; Ji, Xiang-hua; Jiang, Ming-xing; Cheng, Jia-an

2008-01-01

The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest of rice Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to rice, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the rice. At tillering stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 2~4 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice. PMID:18600788

Understanding him in STEM: Sharing the stories of African American male scholars in engineering academic programs at a predominantly White university

NASA Astrophysics Data System (ADS)

Hayes, Robert E., III

Globalization of the world economy has confirmed the need for citizens to exemplify competitive capacities in science, technology, engineering, and mathematics fields. Since the 1970s, American higher education has seen increasing numbers of students entering college but has witnessed a decline in the number of students enrolling in STEM programs. African American men fall behind other students in regards to academic performance, persistence, and success throughout primary, secondary, and tertiary schooling. Accordingly, participation of African American men in STEM disciplines is low in comparison to White males and other race groups. Various factors have been identified as contributing to the academic failures of Black men. Poor academic and social preparedness, racial identity issues, institutional climates, negative stereotypes, and fear of success have been cited as potential contributors to the relative invisibility of African American men in STEM disciplines. This study explores the life stories of five African American male scholars in the college of engineering at a predominantly white university. The goal of the qualitative investigation is to help university faculty and administrators understand the institutional, interpersonal, and collective mechanisms influencing the success identities of African American male undergraduates in STEM academic programs. Understanding the lived experiences of this population may help universities innovate stronger supports for men of color in college and broaden the borders for all students interested in STEM careers.

Do rice water weevils and rice stem borers compete when sharing a host plant?

PubMed

Shi, Sheng-Wei; He, Yan; Ji, Xiang-Hua; Jiang, Ming-Xing; Cheng, Jia-An

2008-07-01

The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest of rice Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to rice, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the rice. At tillering stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 2~4 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice.

Bayer Facts of Science Education XV: A View from the Gatekeepers--STEM Department Chairs at America's Top 200 Research Universities on Female and Underrepresented Minority Undergraduate STEM Students

ERIC Educational Resources Information Center

Journal of Science Education and Technology, 2012

2012-01-01

Diversity and the underrepresentation of women, African-Americans, Hispanics and American Indians in the nation's science, technology, engineering and mathematics (STEM) fields are the subjects of the XV: A View from the Gatekeepers--STEM Department Chairs at America's Top 200 Research Universities on Female and Underrepresented Minority…

Methodology and preliminary results of evaluating stem displacement and assessing root system architecture of longleaf pine saplings

Treesearch

Shi-Jean S. Sung; Daniel J. Leduc; James D. Haywood; Thomas L. Eberhardt; Mary Anne Sword Sayer; Stanley J. Zarnoch

2012-01-01

A field experiment of the effects of container cavity size and root pruning type on longleaf pine was established in November, 2004, in central Louisiana. Sapling stems were first observed to be leaning after hurricane Gustav (September, 2008) and again in August, 2009. To examine the relationship between stem displacement and root system architecture, a stem-displaced...

Development of STEM Readiness Benchmarks to Assist Educational and Career Decision Making. ACT Research Report Series, 2015 (3)

ERIC Educational Resources Information Center

Mattern, Krista; Radunzel, Justine; Westrick, Paul

2015-01-01

Although about 40% of high school graduates who take the ACT® test express interest in pursuing a career in a science, technology, engineering, and mathematics (STEM) field, the percentage of first-year students in college who declare a STEM major is substantially lower. The pool of prospective STEM workers shrinks further as the majority of STEM…

STEM as "Minority": A Phenomenological Case Study of How Students of Color Perceive Their Experience in a STEM Living-Learning Program

ERIC Educational Resources Information Center

Sriram, Rishi; Diaz, Crystal

2016-01-01

The future of the U.S. scientific workforce depends on graduating college students in science, technology, engineering, and math (STEM) fields. The completion rate of STEM students is a national concern, especially among students of color. This qualitative study examines the experiences of students of color in a living-learning program for STEM…

Profile of Pre-Service Science Teachers Based on STEM Career Interest Survey

NASA Astrophysics Data System (ADS)

Winarno, N.; Widodo, A.; Rusdiana, D.; Rochintaniawati, D.; Afifah, R. M. A.

2017-09-01

This study aims to investigate the profile of pre-service science teachers based on STEM (Science, Technology, Engineering, and Mathematics) Career Interest Survey. The study uses descriptive survey method as the research design. Samples collected from 66 preservice science teachers in a university located in Bandung, Indonesia. The results of the study are the profile of pre-service science teachers based on STEM Career Interest Survey shows that the average number of career interest in the field of technology is 4.08, in science 3.80, mathematics 3.39 and engineering 3.30. Pre-service science teachers are found to have interests in the STEM career fields. This research is necessary as there are many instances of people choosing majors or studies that are not in accordance with their interests and talents. The recommendation of this study is to develop learning in pre-service science teachers by using STEM approach.

Women in STEM at a California Community College: A Case Study

ERIC Educational Resources Information Center

Whelan, Garrett C.

2017-01-01

Women are underrepresented in the fields of Science, Technology, Engineering and Mathematics (STEM) from high school to college and into employment. As these are the fastest growing and highest paying fields of the economy this means women are missing the large economic benefits. While there has been a great deal of research into female STEM…

Construction and Contextualization of Authority in STEM Fields: An Exploratory Paper

ERIC Educational Resources Information Center

Kuglitsch, Rebecca; Bordeaux, Julia R.

2017-01-01

In this paper, we explore how librarians can teach students to deconstruct the concept of authority using questions and considering contextual needs in STEM fields. We argue that examining the complexities of common signifiers of authority, such as peer review, citation rates, and types of sources, as well as exploring contextual factors such as…

Moving beyond Cultural Barriers: Successful Strategies of Female Technology Education Teachers

ERIC Educational Resources Information Center

McCarthy, Raymond R.; Berger, Joseph

2008-01-01

Women are underrepresented in Science, Technology, Engineering, and Math (STEM) fields of study and careers with a subset of STEM--Technology Education--possibly one of the least integrated fields for women as students and as professionals. What accounts for this situation and what are potential remedies? The purpose of this study was to learn…

Increasing Access of Female Students in Science Technology, Engineering and Mathematics (STEM), in the University of Malawi (UNIMA)

ERIC Educational Resources Information Center

Mbano, Nellie; Nolan, Kathleen

2017-01-01

In Malawi, in spite of a number of gender equity policies and initiatives that encourage females to pursue careers within the fields of science, technology, engineering, and mathematics (STEM), research indicates that they are under-represented in these fields. One initiative recommended to address the factors contributing to this…

Stem cells: roadmap to the clinic

PubMed Central

Daley, George Q.

2010-01-01

Over the last decade, a remarkable number of papers have been published in which the biology of stem cells is introduced with words and phrases such as “promise,” “rapid progress,” and “future therapies.” To separate myth and hype from reality, the articles in this Stem Cells Review series comprise a rich resource on the state of this fast-paced field and provide a balanced perspective on some of the major advances. They recount what the field has achieved over the past decade and where the field is headed. They also highlight the challenges to be faced in translating what is indeed highly promising science into proven therapies that will regenerate and repair diseased tissues. PMID:20051631

Fitting in or Opting Out: A Review of Key Social-Psychological Factors Influencing a Sense of Belonging for Women in Physics

ERIC Educational Resources Information Center

Lewis, Karyn L.; Stout, Jane G.; Pollack, Steven J.; Finkelstein, Noah D.; Ito, Tiffany A.

2016-01-01

A number of cultural, social, environmental, and biological factors have been suggested to explain women's relatively lower representation in physics and other science, technology, engineering, and math (STEM) fields. Given its persistence, the causes of gender disparities are likely to be complex and multiply determined. In this review paper, we…

Enriching Students' Scientific Thinking through Relational Reasoning: Seeking Evidence in Texts, Tasks, and Talk

ERIC Educational Resources Information Center

Murphy, P. Karen; Firetto, Carla M.; Greene, Jeffrey A.

2017-01-01

As reflected in the Next Generation Science Standards, concerns about the adequacy of education and career preparation in science, technology, engineering, and mathematics (STEM) fields have led to fundamental shifts in the focus of K-12 science education. Such shifts are also highlighted in many of the articles within this special issue, and the…

State performance in pluripotent and adult stem cell research, 2009-2016.

PubMed

Surani, Sana H; Levine, Aaron D

2018-04-01

To examine how the geographic distribution of pluripotent and adult stem cell research publications within the USA differs from other areas of biomedical research. Publication count data for pluripotent stem cell research, adult stem cell research and a comparison group representative of biomedical research more broadly were collected and analyzed for each US state from 2009 to 2016. The distribution of pluripotent stem cell research differed from the other fields with overperformance in pluripotent stem cell research observed in California, as well as Wisconsin, Massachusetts, Maryland and Connecticut. Our analysis suggests that permissive state stem cell policy may be one of the several factors contributing to strong state performance in pluripotent stem cell research.

Rapid Scientific Promotion of Scientific Productions in Stem Cells According to The Indexed Papers in The ISI (web of knowledge).

PubMed

Alijani, Rahim

2015-01-01

In recent years emphasis has been placed on evaluation studies and the publication of scientific papers in national and international journals. In this regard the publication of scientific papers in journals in the Institute for Scientific Information (ISI) database is highly recommended. The evaluation of scientific output via articles in journals indexed in the ISI database will enable the Iranian research authorities to allocate and organize research budgets and human resources in a way that maximises efficient science production. The purpose of the present paper is to publish a general and valid view of science production in the field of stem cells. In this research, outputs in the field of stem cell research are evaluated by survey research, the method of science assessment called Scientometrics in this branch of science. A total of 1528 documents was extracted from the ISI database and analysed using descriptive statistics software in Excel. The results of this research showed that 1528 papers in the stem cell field in the Web of Knowledge database were produced by Iranian researchers. The top ten Iranian researchers in this field have produced 936 of these papers, equivalent to 61.3% of the total. Among the top ten, Soleimani M. has occupied the first place with 181 papers. Regarding international scientific participation, Iranian researchers have cooperated to publish papers with researchers from 50 countries. Nearly 32% (452 papers) of the total research output in this field has been published in the top 10 journals. These results show that a small number of researchers have published the majority of papers in the stem cell field. International participation in this field of research unacceptably low. Such participation provides the opportunity to import modern science and international experience into Iran. This not only causes scientific growth, but also improves the research and enhances opportunities for employment and professional development. Iranian scientific outputs from stem cell research should not be limited to only a few specific journals.

Students' Attitude towards STEM Education

ERIC Educational Resources Information Center

Popa, Roxana-Alexandra; Ciascai, Liliana

2017-01-01

STEM is the acronym of Science, Technology, Engineering, and Mathematics fields. STEM education refers both to teaching and learning in the above-mentioned disciplines, but especially to teaching and learning a new discipline based on the integration of Science, Technology, Engineering, and Mathematics. The present survey aims to investigate the…

The Hidden STEM Economy

ERIC Educational Resources Information Center

Rothwell, Jonathan

2013-01-01

Workers in STEM (science, technology, engineering, and math) fields play a direct role in driving economic growth. Yet, because of how the STEM economy has been defined, policymakers have mainly focused on supporting workers with at least a bachelor's (BA) degree, overlooking a strong potential workforce of those with less than a BA. This report…

STEM | News

Science.gov Websites

the field. STEM Career Expo at Fermilab From NCTV17, April 20, 2018: The next generation of scientists Career Expo. Watch the 90-second segment. In photos: Dare to Dream shares joys of STEM with Latina middle Lab professionals discussed their work, shared their experiences in different career areas and

Advancing the "E" in K-12 STEM Education

ERIC Educational Resources Information Center

Rockland, Ronald; Bloom, Diane S.; Carpinelli, John; Burr-Alexander, Levelle; Hirsch, Linda S.; Kimmel, Howard

2010-01-01

Technological fields, like engineering, are in desperate need of more qualified workers, yet not enough students are pursuing studies in science, technology, engineering, or mathematics (STEM) that would prepare them for technical careers. Unfortunately, many students have no interest in STEM careers, particularly engineering, because they are not…

Diversifying the STEM Pipeline: Recommendations from the Model Replication Institutions Program

ERIC Educational Resources Information Center

Institute for Higher Education Policy, 2010

2010-01-01

Launched in 2006 to address issues of national competitiveness and equity in science, technology, engineering, and mathematics (STEM) fields, the National Science Foundation-funded Model Replication Institutions (MRI) program sought to improve the quality, availability, and diversity of STEM education. The project offered technical assistance to…

Too Few or Perhaps Too Many STEM Graduates

ERIC Educational Resources Information Center

Birrell, Bob

2015-01-01

Industry bodies, research and educational organisations have lobbied intensely for increased funding for training in the STEM disciplines. It is time to reassess this advocacy. Undergraduate commencements in STEM fields have increased strongly since 2009, yet the current employment prospects for these graduates are poor. Advocates have not made a…

iSTEM: Learning Mathematics through Minecraft

ERIC Educational Resources Information Center

Bos, Beth; Wilder, Lucy; Cook, Marcelina; O'Donnell, Ryan

2014-01-01

The Common Core State Standards can be taught with Minecraft, an interactive creative Lego®-like game. Integrating Science, Technology, Engineering, and Mathematics (iSTEM) authors share ideas and activities that stimulate student interest in the integrated fields of science, technology, engineering, and mathematics (STEM) in K-grade 6 classrooms.

Family Friendly Policies in STEM Departments: Awareness and Determinants

ERIC Educational Resources Information Center

Su, Xuhong; Bozeman, Barry

2016-01-01

Focused on academic departments in science, technology, engineering, and mathematics (STEM) fields in the United States, we attempt to map department chairs' awareness of family friendly policies and investigate possible determinants of their knowledge levels. Based on a sample of STEM department chairs in American research universities, we find…

Behavioural responses of wheat stem sawflies to wheat volatiles

Treesearch

D. Piesik; D. K. Weaver; J. B. Runyon; M. Buteler; G. E. Peck; W. L. Morrill

2008-01-01

1) Adult wheat stem sawflies Cephus cinctus, pests of cultivated cereals that also infests wild grasses, migrate into wheat fields where they oviposit in elongating, succulent stems. 2) Volatiles released by wheat plants at susceptible stages were analyzed to determine potential semiochemical compounds. Seven major compounds were identified and...

Using Technology to Support STEM Reading

ERIC Educational Resources Information Center

Schneps, Matthew H.; O'Keeffe, Jamie K.; Heffner-Wong, Amanda; Sonnert, Gerhard

2010-01-01

Tasks in science, technology, engineering, and mathematics (STEM) are unusually varied because they target phenomena occurring in diverse domains and call upon a wide range of abilities to perform them. The fact that STEM tasks cover such a broad spectrum of abilities makes these fields uncharacteristically inclusive: Individuals with disabilities…

STEM Motivation Interventions for Adolescents: A Promising Start, but Further to Go

ERIC Educational Resources Information Center

Rosenzweig, Emily Q.; Wigfield, Allan

2016-01-01

One way to increase students' participation in science, technology, engineering, and mathematics (STEM) fields is to target their motivation. Researchers have conducted a growing number of interventions addressing students' motivation in STEM; however, this body of work has not been adequately reviewed. We systematically reviewed experimental and…

Documenting Instructional Practices in Large Introductory STEM Lecture Courses

ERIC Educational Resources Information Center

Vu, Viet Quoc

2017-01-01

STEM education reform in higher education is framed around the need to improve student learning outcomes, increase student retention, and increase the number of underrepresented minorities and female students in STEM fields, all of which would ultimately contribute to America's competitiveness and prosperity. To achieve these goals, education…

An Educative, Values-Engaged Approach to Evaluating STEM Educational Programs

ERIC Educational Resources Information Center

Greene, Jennifer C.; DeStefano, Lizanne; Burgon, Holli; Hall, Jori

2006-01-01

There is concern that the nation's schools are not preparing students to excel in science, technology, engineering, and mathematics (STEM) fields. At both precollege and postsecondary levels, much effort is needed to create and implement powerful STEM curricula, prepare and support highly qualified teachers, deliver effective instruction, and give…

National Academies Piece on Integrated STEM

ERIC Educational Resources Information Center

Pearson, Greg

2017-01-01

Advocates of more connected approaches to teaching the STEM subjects argue that such integration, especially in the context of real-world issues, can make these fields more relevant to students and ultimately increase their motivation and achievement. However, there is little research on how best to integrate the STEM disciplines or on what…

Trends in the human embryonic stem cell patent field.

PubMed

Karlsson, Ulrika; Hyllner, Johan; Runeberg, Kristina

2007-01-01

The successful derivation of human embryonic stem (hES) cell lines in late 1990s marks the birth of a new era in biomedical research. In the USA, this landmark invention is protected by granted composition-of-matter patents. In addition to these patents, several others have been granted on further development of hES cell research, such as on differentiated cell types and in vitro and in vivo use aspects. In Europe, there is presently no consensus pertaining to the patentability of hES cells, and all patent applications pending at the European patent office are therefore awaiting a principal decision by the Enlarged Board of Appeal. The authors argue that it will be of importance to the stem cell industry that patents are granted on inventions downstream in the value chain, e.g on specialised cell types derived from hES cells and different drug discovery applications. Patents and patent applications on such inventions for the three germ layers ectoderm/neuro, endoderm/hepato and mesoderm/cardio have been examined. The number of patents increased in the period 2001 to 2006 for all three lineages with ectoderm/neuro as the most patent intensive field. There where 9-13 times more US patent applications filed related to the three lineages compared to in Europe.

Human Embryonic and Induced Pluripotent Stem Cell Research Trends: Complementation and Diversification of the Field

PubMed Central

Kobold, Sabine; Guhr, Anke; Kurtz, Andreas; Löser, Peter

2015-01-01

Summary Research in human induced pluripotent stem cells (hiPSCs) is rapidly developing and there are expectations that this research may obviate the need to use human embryonic stem cells (hESCs), the ethics of which has been a subject of controversy for more than 15 years. In this study, we investigated approximately 3,400 original research papers that reported an experimental use of these types of human pluripotent stem cells (hPSCs) and were published from 2008 to 2013. We found that research into both cell types was conducted independently and further expanded, accompanied by a growing intersection of both research fields. Moreover, an in-depth analysis of papers that reported the use of both cell types indicates that hESCs are still being used as a “gold standard,” but in a declining proportion of publications. Instead, the expanding research field is diversifying and hESC and hiPSC lines are increasingly being used in more independent research and application areas. PMID:25866160

Development and Evaluation of Glycine max Germplasm Lines with Quantitative Resistance to Sclerotinia sclerotiorum

PubMed Central

McCaghey, Megan; Willbur, Jaime; Ranjan, Ashish; Grau, Craig R.; Chapman, Scott; Diers, Brian; Groves, Carol; Kabbage, Mehdi; Smith, Damon L.

2017-01-01

Sclerotinia sclerotiorum, the causal agent of Sclerotinia stem rot, is a devastating fungal pathogen of soybean that can cause significant yield losses to growers when environmental conditions are favorable for the disease. The development of resistant varieties has proven difficult. However, poor resistance in commercial cultivars can be improved through additional breeding efforts and understanding the genetic basis of resistance. The objective of this project was to develop soybean germplasm lines that have a high level of Sclerotinia stem rot resistance to be used directly as cultivars or in breeding programs as a source of improved Sclerotinia stem rot resistance. Sclerotinia stem rot-resistant soybean germplasm was developed by crossing two sources of resistance, W04-1002 and AxN-1-55, with lines exhibiting resistance to Heterodera glycines and Cadophora gregata in addition to favorable agronomic traits. Following greenhouse evaluations of 1,076 inbred lines derived from these crosses, 31 lines were evaluated for resistance in field tests during the 2014 field season. Subsequently, 11 Sclerotinia stem rot resistant breeding lines were moved forward for field evaluation in 2015, and seven elite breeding lines were selected and evaluated in the 2016 field season. To better understand resistance mechanisms, a marker analysis was conducted to identify quantitative trait loci linked to resistance. Thirteen markers associated with Sclerotinia stem rot resistance were identified on chromosomes 15, 16, 17, 18, and 19. Our markers confirm previously reported chromosomal regions associated with Sclerotinia stem rot resistance as well as a novel region of chromosome 16. The seven elite germplasm lines were also re-evaluated within a greenhouse setting using a cut petiole technique with multiple S. sclerotiorum isolates to test the durability of physiological resistance of the lines in a controlled environment. This work presents a novel and comprehensive classical breeding method for selecting lines with physiological resistance to Sclerotinia stem rot and a range of agronomic traits. In these studies, we identify four germplasm lines; 91–38, 51–23, SSR51–70, and 52–82B exhibiting a high level of Sclerotinia stem rot resistance combined with desirable agronomic traits, including high protein and oil contents. The germplasm identified in this study will serve as a valuable source of physiological resistance to Sclerotinia stem rot that could be improved through further breeding to generate high-yielding commercial soybean cultivars. PMID:28912790

Recent progress in stem cell differentiation directed by material and mechanical cues.

PubMed

Lin, Xunxun; Shi, Yuan; Cao, Yilin; Liu, Wei

2016-02-02

Stem cells play essential roles in tissue regeneration in vivo via specific lineage differentiation induced by environmental factors. In the past, biochemical signals were the focus of induced stem cell differentiation. As reported by Engler et al (2006 Cell 126 677-89), biophysical signal mediated stem cell differentiation could also serve as an important inducer. With the advancement of material science, it becomes a possible strategy to generate active biophysical signals for directing stem cell fate through specially designed material microstructures. In the past five years, significant progress has been made in this field, and these designed biophysical signals include material elasticity/rigidity, micropatterned structure, extracellular matrix (ECM) coated materials, material transmitted extracellular mechanical force etc. A large number of investigations involved material directed differentiation of mesenchymal stem cells, neural stem/progenitor cells, adipose derived stem cells, hematopoietic stem/progenitor cells, embryonic stem cells and other cells. Hydrogel based materials were commonly used to create varied mechanical properties via modifying the ratio of different components, crosslinking levels, matrix concentration and conjugation with other components. Among them, polyacrylamide (PAM) and polydimethylsiloxane (PDMS) hydrogels remained the major types of material. Specially designed micropatterning was not only able to create a unique topographical surface to control cell shape, alignment, cell-cell and cell-matrix contact for basic stem cell biology study, but also could be integrated with 3D bioprinting to generate micropattered 3D structure and thus to induce stem cell based tissue regeneration. ECM coating on a specific topographical structure was capable of inducing even more specific and potent stem cell differentiation along with soluble factors and mechanical force. The article overviews the progress of the past five years in this particular field.

Stem cell clinical trials for spinal cord injury: readiness, reluctance, redefinition.

PubMed

Illes, J; Reimer, J C; Kwon, B K

2011-11-01

A wealth of scientific and clinical research has focused on the use of stem cells as a potential therapy for spinal cord injury (SCI), culminating most recently in the initiation of clinical trials. However, with the urgency that scientists and clinicians have undertaken to move forward with novel therapies for this devastating injury, the perspectives of stakeholders who live with a SCI have been left behind. Translational research in this rapidly growing field therefore overlooks a critically important viewpoint. We address this concern with a qualitative study of the perspectives on experimental stem cell treatments from individuals who have actually suffered a spinal cord injury. Using focus groups and interviews, we engaged individuals with thoracic and cervical SCIs at sub-acute and chronic stages post-injury. We found four major themes that inform the progression of stem cell research to clinical trials: 'readiness', 'the here and now', 'wait and see', and 'informed hope'. Taken together, the data suggest a profound difference related to target timing of stem cell clinical trials and the perspectives about timing from those who are the end-beneficiaries of therapy. To bridge this gap, we conclude with a number of considerations for the timing disparity of trials and recommendations for improving informed consent.

Employment of the Triple Helix concept for development of regenerative medicine applications based on human pluripotent stem cells

PubMed Central

2014-01-01

Using human pluripotent stem cells as a source to generate differentiated progenies for regenerative medicine applications has attracted substantial interest during recent years. Having the capability to produce large quantities of human cells that can replace damaged tissue due to disease or injury opens novel avenues for relieving symptoms and also potentially offers cures for many severe human diseases. Although tremendous advancements have been made, there is still much research and development left before human pluripotent stem cell derived products can be made available for cell therapy applications. In order to speed up the development processes, we argue strongly in favor of cross-disciplinary collaborative efforts which have many advantages, especially in a relatively new field such as regenerative medicine based on human pluripotent stem cells. In this review, we aim to illustrate how some of the hurdles for bringing human pluripotent stem cell derivatives from bench-to-bed can be effectively addressed through the establishment of collaborative programs involving academic institutions, biotech industries, and pharmaceutical companies. By taking advantage of the strengths from each organization, innovation and productivity can be maximized from a resource perspective and thus, the chances of successfully bringing novel regenerative medicine treatment options to patients increase. PMID:24872863

Human dental pulp stem cells: Applications in future regenerative medicine

PubMed Central

Potdar, Pravin D; Jethmalani, Yogita D

2015-01-01

Stem cells are pluripotent cells, having a property of differentiating into various types of cells of human body. Several studies have developed mesenchymal stem cells (MSCs) from various human tissues, peripheral blood and body fluids. These cells are then characterized by cellular and molecular markers to understand their specific phenotypes. Dental pulp stem cells (DPSCs) are having a MSCs phenotype and they are differentiated into neuron, cardiomyocytes, chondrocytes, osteoblasts, liver cells and β cells of islet of pancreas. Thus, DPSCs have shown great potentiality to use in regenerative medicine for treatment of various human diseases including dental related problems. These cells can also be developed into induced pluripotent stem cells by incorporation of pluripotency markers and use for regenerative therapies of various diseases. The DPSCs are derived from various dental tissues such as human exfoliated deciduous teeth, apical papilla, periodontal ligament and dental follicle tissue. This review will overview the information about isolation, cellular and molecular characterization and differentiation of DPSCs into various types of human cells and thus these cells have important applications in regenerative therapies for various diseases. This review will be most useful for postgraduate dental students as well as scientists working in the field of oral pathology and oral medicine. PMID:26131314