Type II Radio Bursts Observed by STEREO/Waves and Wind/Waves instruments

NASA Astrophysics Data System (ADS)

Krupar, V.; Magdalenic, J.; Zhukov, A.; Rodriguez, L.; Mierla, M.; Maksimovic, M.; Cecconi, B.; Santolik, O.

2013-12-01

Type II radio bursts are slow-drift emissions triggered by suprathermal electrons accelerated on shock fronts of propagating CMEs. We present several events at kilometric wavelengths observed by radio instruments onboard the STEREO and Wind spacecraft. The STEREO/Waves and Wind/Waves have goniopolarimetric (GP, also referred to as direction finding) capabilities that allow us to triangulate radio sources when an emission is observed by two or more spacecraft. As the GP inversion has high requirements on the signal-to-noise ratio we only have a few type II radio bursts with sufficient intensity for this analysis. We have compared obtained radio sources with white-light observations of STEREO/COR and STEREO/HI instruments. Our preliminary results indicate that radio sources are located at flanks of propagating CMEs.

The Stereo Electron Spikes and the Interplanetary Magnetic Field

NASA Astrophysics Data System (ADS)

Jokipii, J. R.; Sheeley, N. R., Jr.; Wang, Y. M.; Giacalone, J.

2016-12-01

A recent paper (Klassen etal, 2015) discussed observations of a spike event of 55-65 keV electrons which occurred very nearly simultaneously at STEREO A and STEREO B, which at the time were separated in longitude by 38 degrees. The authors associated the spikes with a flare at the Sun near the footpoint of the nominal Archimedean spiral magnetic field line passing through STEREO A. The spike at STEREO A was delayed by 2.2 minutes from that at STEREOB. We discuss the observations in terms of a model in which the electrons, accelerated at the flare, propagate without significant scattering along magnetic field lines which separate or diverge as a function of radial distance from the Sun. The near simultaneity of the spikes at the two spacecraft is a natural consequence of this model. We interpret the divergence of the magnetic field lines as a consequence of field-line random walk and flux-tube expansion. We show that the field-line random walk in the absence of flux-tube expansion produces an rms spread of field lines significantly less than that which is required to produce to observed divergence. We find that observations of the solar wind and its source region at the time of the event can account for the observations in terms of propagation along interplanetary magnetic field-lines. Klassen, A., Dresing, N., Gomez-Herrero, R, and Heber, B., A&A 580, A115 (2015) Financial support for NS and YMW was provided by NASA and CNR.

Large Amplitude Whistler Waves and Electron Acceleration in the Earth's Radiation Belts: A Review of STEREO and Wind Observations

NASA Technical Reports Server (NTRS)

Cattell, Cynthia; Breneman, A.; Goetz, K.; Kellogg, P.; Kersten, K.; Wygant, J.; Wilson, L. B., III; Looper, Mark D.; Blake, J. Bernard; Roth, I.

2012-01-01

One of the critical problems for understanding the dynamics of Earth's radiation belts is determining the physical processes that energize and scatter relativistic electrons. We review measurements from the Wind/Waves and STEREO S/Waves waveform capture instruments of large amplitude whistler-mode waves. These observations have provided strong evidence that large amplitude (100s mV/m) whistler-mode waves are common during magnetically active periods. The large amplitude whistlers have characteristics that are different from typical chorus. They are usually nondispersive and obliquely propagating, with a large longitudinal electric field and significant parallel electric field. We will also review comparisons of STEREO and Wind wave observations with SAMPEX observations of electron microbursts. Simulations show that the waves can result in energization by many MeV and/or scattering by large angles during a single wave packet encounter due to coherent, nonlinear processes including trapping. The experimental observations combined with simulations suggest that quasilinear theoretical models of electron energization and scattering via small-amplitude waves, with timescales of hours to days, may be inadequate for understanding radiation belt dynamics.

Combined application of electron backscatter diffraction and stereo-photogrammetry in fractography studies.

PubMed

Davies, P A; Randle, V

2001-10-01

The main aim of this paper is to report on recent experimental developments that have succeeded in combining electron back-scatter diffraction (EBSD) with stereo-photogrammetry, compared with two other methods for study of fracture surfaces, namely visual fractography analysis in the scanning electron microscope (SEM) and EBSD directly from facets. These approaches will be illustrated with data relating to the cleavage plane orientation analysis in a ferritic and C-Mn steel. It is demonstrated that the combined use of EBSD and stereo-photogrammetry represents a significant advance in the methodology for facet crystallography analysis. The results of point counting from fractograph characterization determined that the proportions of intergranular fracture in C-Mn and ferritic steels were 10.4% and 9.4%, respectively. The crystallographic orientation was determined directly from the fracture surface of a ferritic steel sample and produced an orientation distribution with a clear trend towards the [001] plane. A stereo-photogrammetry technique was validated using the known geometry of a Vickers hardness indent. The technique was then successfully employed to measure the macroscopic orientation of individual cleavage facets in the same reference frame as the EBSD measurements. Correlating the results of these measurements indicated that the actual crystallographic orientation of every cleavage facet identified in the steel specimens is [001].

Analysis of tooth marks in a homicide case. Observations by means of visual description, stereo-photography, scanning electron microscopy and stereometric graphic plotting.

PubMed

Bang, G

1976-01-01

In 1957 a woman was murdered in Oslo. Her left breast exhibited tooth marks. A man was arrested and sentenced to life imprisonment partly because of the dental evidence. He never admitted guilt and filed a petition for retrial. The present author was appointed as new dental expert. The material consisted of the fixed breast, models of the bite mark and models of the teeth of the convict, and several photographs. By means of visual examination, a magnifying glass, a lens stereoscope and a stereomicroscope characteristic details were noted. Stereoscopic picture pairs were taken, the material was studied by means of scanning electron microscopy and a stereometricgraphic plotting method permitting the outline of the tooth mark or the biting edge of a tooth to be registered in great detail in all three dimensions in the form of a contour map. This method has not previously been applied in the analysis of tooth marks in human skin. These examinations revealed no discrepancies but showed many corresponding characteristic features between the tooth marks and the teeth of the convict, resulting in the conclusion that it is highly probable that the tooth marks in the breast were made by the teeth of the convict.

Comparative evaluation of topographical data of dental implant surfaces applying optical interferometry and scanning electron microscopy.

PubMed

Kournetas, N; Spintzyk, S; Schweizer, E; Sawada, T; Said, F; Schmid, P; Geis-Gerstorfer, J; Eliades, G; Rupp, F

2017-08-01

Comparability of topographical data of implant surfaces in literature is low and their clinical relevance often equivocal. The aim of this study was to investigate the ability of scanning electron microscopy and optical interferometry to assess statistically similar 3-dimensional roughness parameter results and to evaluate these data based on predefined criteria regarded relevant for a favorable biological response. Four different commercial dental screw-type implants (NanoTite Certain Prevail, TiUnite Brånemark Mk III, XiVE S Plus and SLA Standard Plus) were analyzed by stereo scanning electron microscopy and white light interferometry. Surface height, spatial and hybrid roughness parameters (Sa, Sz, Ssk, Sku, Sal, Str, Sdr) were assessed from raw and filtered data (Gaussian 50μm and 5μm cut-off-filters), respectively. Data were statistically compared by one-way ANOVA and Tukey-Kramer post-hoc test. For a clinically relevant interpretation, a categorizing evaluation approach was used based on predefined threshold criteria for each roughness parameter. The two methods exhibited predominantly statistical differences. Dependent on roughness parameters and filter settings, both methods showed variations in rankings of the implant surfaces and differed in their ability to discriminate the different topographies. Overall, the analyses revealed scale-dependent roughness data. Compared to the pure statistical approach, the categorizing evaluation resulted in much more similarities between the two methods. This study suggests to reconsider current approaches for the topographical evaluation of implant surfaces and to further seek after proper experimental settings. Furthermore, the specific role of different roughness parameters for the bioresponse has to be studied in detail in order to better define clinically relevant, scale-dependent and parameter-specific thresholds and ranges. Copyright © 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Stereo-selective binding of chlorobenzene on Si(111)-7×7

NASA Astrophysics Data System (ADS)

Cao, Y.; Deng, J. F.; Xu, G. Q.

2000-03-01

The adsorption and binding of chlorobenzene (C6H5Cl) on clean and D-modified Si(111)-7×7 surfaces have been investigated using high resolution electron energy loss spectroscopy (HREELS) and thermal desorption spectroscopy (TDS). On a clean surface, both chemisorbed and physisorbed C6H5Cl are observed at an adsorption temperature of 110 K. The HREEL spectra show direct evidence for the presence of both sp2 and sp3 carbon atoms in chemisorbed C6H5Cl molecules on Si(111)-7×7. Upon D-modification, the chemisorption of C6H5Cl decreases rapidly with increasing D-coverage (θD). At θD=1/3 ML, only physisorbed chlorobenzene is detected, which strongly suggests the involvement of rest-atoms in the chemisorption of C6H5Cl. Combined with the scanning tunneling microscopy (STM) results by Chen et al. [Surf. Sci. 340, 224 (1995)] showing the participation of adatoms in the binding, we propose that the 2,5-carbon atoms in C6H5Cl are stereo-selectively di-σ bonded to a pair of adjacent adatom and rest-atom on the Si(111)-7×7 surface, yielding a 2,5-chlorocyclohexadienelike surface adduct.

STEREO In-situ Data Analysis

NASA Astrophysics Data System (ADS)

Schroeder, P. C.; Luhmann, J. G.; Davis, A. J.; Russell, C. T.

2006-12-01

STEREO's IMPACT (In-situ Measurements of Particles and CME Transients) investigation provides the first opportunity for long duration, detailed observations of 1 AU magnetic field structures, plasma and suprathermal electrons, and energetic particles at points bracketing Earth's heliospheric location. The PLASTIC instrument takes plasma ion composition measurements completing STEREO's comprehensive in-situ perspective. Stereoscopic/3D information from the STEREO SECCHI imagers and SWAVES radio experiment make it possible to use both multipoint and quadrature studies to connect interplanetary Coronal Mass Ejections (ICME) and solar wind structures to CMEs and coronal holes observed at the Sun. The uniqueness of the STEREO mission requires novel data analysis tools and techniques to take advantage of the mission's full scientific potential. An interactive browser with the ability to create publication-quality plots has been developed which integrates STEREO's in-situ data with data from a variety of other missions including WIND and ACE. Also, an application program interface (API) is provided allowing users to create custom software that ties directly into STEREO's data set. The API allows for more advanced forms of data mining than currently available through most web-based data services. A variety of data access techniques and the development of cross-spacecraft data analysis tools allow the larger scientific community to combine STEREO's unique in-situ data with those of other missions, particularly the L1 missions, and, therefore, to maximize STEREO's scientific potential in gaining a greater understanding of the heliosphere.

Multipoint connectivity analysis of the May 2007 solar energetic particle events

NASA Astrophysics Data System (ADS)

Chollet, E. E.; Mewaldt, R. A.; Cummings, A. C.; Gosling, J. T.; Haggerty, D. K.; Hu, Q.; Larson, D.; Lavraud, B.; Leske, R. A.; Opitz, A.; Roelof, E. C.; Russell, C. T.; Sauvaud, J.-A.

2010-12-01

In May of 2007, the STEREO Ahead and Behind spacecraft, along with the ACE spacecraft situated between the two STEREO spacecraft, observed two small solar energetic particle (SEP) events. STEREO-A and -B observed nearly identical time profiles in the 19 May event, but in the 23 May event, the protons arrived significantly earlier at STEREO-A than at STEREO-B and the time-intensity profiles were markedly different. We present SEP anisotropy, suprathermal electron pitch angle and solar wind data to demonstrate distortion in the magnetic field topology produced by the passage of multiple interplanetary coronal mass ejections on 22 and 23 May, causing the two spacecraft to magnetically connect to different points back at the Sun. This pair of events illustrates the power of multipoint observations in detailed interpretation of complex events, since only a small shift in observer location results in different magnetic field line connections and different SEP time-intensity profiles.

Assessment of engineered surfaces roughness by high-resolution 3D SEM photogrammetry.

PubMed

Gontard, L C; López-Castro, J D; González-Rovira, L; Vázquez-Martínez, J M; Varela-Feria, F M; Marcos, M; Calvino, J J

2017-06-01

We describe a methodology to obtain three-dimensional models of engineered surfaces using scanning electron microscopy and multi-view photogrammetry (3DSEM). For the reconstruction of the 3D models of the surfaces we used freeware available in the cloud. The method was applied to study the surface roughness of metallic samples patterned with parallel grooves by means of laser. The results are compared with measurements obtained using stylus profilometry (PR) and SEM stereo-photogrammetry (SP). The application of 3DSEM is more time demanding than PR or SP, but it provides a more accurate representation of the surfaces. The results obtained with the three techniques are compared by investigating the influence of sampling step on roughness parameters. Copyright © 2017 Elsevier B.V. All rights reserved.

In situ analysis of the organic framework in the prismatic layer of mollusc shell.

PubMed

Tong, Hua; Hu, Jiming; Ma, Wentao; Zhong, Guirong; Yao, Songnian; Cao, Nianxing

2002-06-01

A novel in situ analytic approach was constructed by means of ion sputtering, decalcification and deprotein techniques combining with scanning electron microscopy (SEM) and transmission electron microscope (TEM) ultrastructural analysis. The method was employed to determine the spatial distribution of the organic framework outside and the inner crystal and organic/inorganic interface spatial geometrical relationship in the prismatic layer of cristaris plicate (leach). The results show that there is a substructure of organic matrix in the intracrystalline region. The prismatic layer forms according to strict hierarchical configuration of regular pattern. Each unit of organic template of prismatic layer can uniquely determine the column crystal growth direction, spatial orientation and size. Cavity templates are responsible for supporting. limiting size and shape and determining the crystal growth spatial orientation, while the intracrystal organic matrix is responsible for providing nucleation point and inducing the nucleation process of calcite. The stereo hierarchical fabrication of prismatic layer was elucidated for the first time.

NASA Tech Briefs, March 2008

NASA Technical Reports Server (NTRS)

2008-01-01

Topics covered include: WRATS Integrated Data Acquisition System; Breadboard Signal Processor for Arraying DSN Antennas; Digital Receiver Phase Meter; Split-Block Waveguide Polarization Twist for 220 to 325 GHz; Nano-Multiplication-Region Avalanche Photodiodes and Arrays; Tailored Asymmetry for Enhanced Coupling to WGM Resonators; Disabling CNT Electronic Devices by Use of Electron Beams; Conical Bearingless Motor/Generators; Integrated Force Method for Indeterminate Structures; Carbon-Nanotube-Based Electrodes for Biomedical Applications; Compact Directional Microwave Antenna for Localized Heating; Using Hyperspectral Imagery to Identify Turfgrass Stresses; Shaping Diffraction-Grating Grooves to Optimize Efficiency; Low-Light-Shift Cesium Fountain without Mechanical Shutters; Magnetic Compensation for Second-Order Doppler Shift in LITS; Nanostructures Exploit Hybrid-Polariton Resonances; Microfluidics, Chromatography, and Atomic-Force Microscopy; Model of Image Artifacts from Dust Particles; Pattern-Recognition System for Approaching a Known Target; Orchestrator Telemetry Processing Pipeline; Scheme for Quantum Computing Immune to Decoherence; Spin-Stabilized Microsatellites with Solar Concentrators; Phase Calibration of Antenna Arrays Aimed at Spacecraft; Ring Bus Architecture for a Solid-State Recorder; and Image Compression Algorithm Altered to Improve Stereo Ranging.

Focus and perspective adaptive digital surgical microscope: optomechanical design and experimental implementation

NASA Astrophysics Data System (ADS)

Claus, Daniel; Reichert, Carsten; Herkommer, Alois

2017-05-01

This paper relates to the improvement of conventional surgical stereo microscopy via the application of digital recording devices and adaptive optics. The research is aimed at improving the working conditions of the surgeon during the operation, such that free head movement is possible. The depth clues known from conventional stereo microscopy in interaction with the human eye's functionality, such as convergence, disparity, angular elevation, parallax, and accommodation, are implemented in a digital recording system via adaptive optomechanical components. Two laterally moving pupil apertures have been used mimicking the digital implementation of the eye's vergence and head motion. The natural eye's accommodation is mimicked via the application of a tunable lens. Additionally, another system has been built, which enables tracking the surgeon's eye pupil through a digital displaying stereoscopic microscope to supply the necessary information for steering the recording system. The optomechanical design and experimental results for both systems, digital recording stereoscopic microscope and pupil tracking system, are shown.

Polarized Light Microscopy

NASA Technical Reports Server (NTRS)

Frandsen, Athela F.

2016-01-01

Polarized light microscopy (PLM) is a technique which employs the use of polarizing filters to obtain substantial optical property information about the material which is being observed. This information can be combined with other microscopy techniques to confirm or elucidate the identity of an unknown material, determine whether a particular contaminant is present (as with asbestos analysis), or to provide important information that can be used to refine a manufacturing or chemical process. PLM was the major microscopy technique in use for identification of materials for nearly a century since its introduction in 1834 by William Fox Talbot, as other techniques such as SEM (Scanning Electron Microscopy), FTIR (Fourier Transform Infrared spectroscopy), XPD (X-ray Powder Diffraction), and TEM (Transmission Electron Microscopy) had not yet been developed. Today, it is still the only technique approved by the Environmental Protection Agency (EPA) for asbestos analysis, and is often the technique first applied for identification of unknown materials. PLM uses different configurations in order to determine different material properties. With each configuration additional clues can be gathered, leading to a conclusion of material identity. With no polarizing filter, the microscope can be used just as a stereo optical microscope, and view qualities such as morphology, size, and number of phases. With a single polarizing filter (single polars), additional properties can be established, such as pleochroism, individual refractive indices, and dispersion staining. With two polarizing filters (crossed polars), even more can be deduced: isotropy vs. anisotropy, extinction angle, birefringence/degree of birefringence, sign of elongation, and anomalous polarization colors, among others. With the use of PLM many of these properties can be determined in a matter of seconds, even for those who are not highly trained. McCrone, a leader in the field of polarized light microscopy, often advised, If you cant determine a specific optical property of a particle after two minutes, move onto another configuration. Since optical properties can be seen so very quickly and easily under polarized light, it is only necessary to spend a maximum of two minutes on a technique to determine a particular property, though often only a few seconds are required.

Television monitor field shifter and an opto-electronic method for obtaining a stereo image of optimal depth resolution and reduced depth distortion on a single screen

NASA Technical Reports Server (NTRS)

Diner, Daniel B. (Inventor)

1989-01-01

A method and apparatus is developed for obtaining a stereo image with reduced depth distortion and optimum depth resolution. Static and dynamic depth distortion and depth resolution tradeoff is provided. Cameras obtaining the images for a stereo view are converged at a convergence point behind the object to be presented in the image, and the collection-surface-to-object distance, the camera separation distance, and the focal lengths of zoom lenses for the cameras are all increased. Doubling the distances cuts the static depth distortion in half while maintaining image size and depth resolution. Dynamic depth distortion is minimized by panning a stereo view-collecting camera system about a circle which passes through the convergence point and the camera's first nodal points. Horizontal field shifting of the television fields on a television monitor brings both the monitor and the stereo views within the viewer's limit of binocular fusion.

STEREO In-situ Data Analysis

NASA Astrophysics Data System (ADS)

Schroeder, P. C.; Luhmann, J. G.; Davis, A. J.; Russell, C. T.

2007-05-01

STEREO's IMPACT (In-situ Measurements of Particles and CME Transients) investigation provides the first opportunity for long duration, detailed observations of 1 AU magnetic field structures, plasma and suprathermal electrons, and energetic particles at points bracketing Earth's heliospheric location. The PLASTIC instrument takes plasma ion composition measurements completing STEREO's comprehensive in-situ perspective. Stereoscopic/3D information from the STEREO SECCHI imagers and SWAVES radio experiment make it possible to use both multipoint and quadrature studies to connect interplanetary Coronal Mass Ejections (ICME) and solar wind structures to CMEs and coronal holes observed at the Sun. The uniqueness of the STEREO mission requires novel data analysis tools and techniques to take advantage of the mission's full scientific potential. An interactive browser with the ability to create publication-quality plots has been developed which integrates STEREO's in-situ data with data from a variety of other missions including WIND and ACE. Static summary plots and a key-parameter type data set with a related online browser provide alternative data access. Finally, an application program interface (API) is provided allowing users to create custom software that ties directly into STEREO's data set. The API allows for more advanced forms of data mining than currently available through most web-based data services. A variety of data access techniques and the development of cross- spacecraft data analysis tools allow the larger scientific community to combine STEREO's unique in-situ data with those of other missions, particularly the L1 missions, and, therefore, to maximize STEREO's scientific potential in gaining a greater understanding of the heliosphere.

Sensor fusion of phase measuring profilometry and stereo vision for three-dimensional inspection of electronic components assembled on printed circuit boards.

PubMed

Hong, Deokhwa; Lee, Hyunki; Kim, Min Young; Cho, Hyungsuck; Moon, Jeon Il

2009-07-20

Automatic optical inspection (AOI) for printed circuit board (PCB) assembly plays a very important role in modern electronics manufacturing industries. Well-developed inspection machines in each assembly process are required to ensure the manufacturing quality of the electronics products. However, generally almost all AOI machines are based on 2D image-analysis technology. In this paper, a 3D-measurement-method-based AOI system is proposed consisting of a phase shifting profilometer and a stereo vision system for assembled electronic components on a PCB after component mounting and the reflow process. In this system information from two visual systems is fused to extend the shape measurement range limited by 2pi phase ambiguity of the phase shifting profilometer, and finally to maintain fine measurement resolution and high accuracy of the phase shifting profilometer with the measurement range extended by the stereo vision. The main purpose is to overcome the low inspection reliability problem of 2D-based inspection machines by using 3D information of components. The 3D shape measurement results on PCB-mounted electronic components are shown and compared with results from contact and noncontact 3D measuring machines. Based on a series of experiments, the usefulness of the proposed sensor system and its fusion technique are discussed and analyzed in detail.

Approaching Solar Maximum 24 with Stereo-Multipoint Observations of Solar Energetic Particle Events

NASA Technical Reports Server (NTRS)

Dresing, N.; Cohen, C. M. S.; Gomez-Herrero, R.; Heber, B.; Klassen, A.; Leske, R. A.; Mason, G. M.; Mewaldt, R. A.; von Rosenvinge, T. T.

2014-01-01

Since the beginning of the Solar Terrestrial Relations Observatory (STEREO) mission at the end of 2006, the two spacecraft have now separated by more than 130? degrees from the Earth. A 360-degree view of the Sun has been possible since February 2011, providing multipoint in situ and remote sensing observations of unprecedented quality. Combining STEREO observations with near-Earth measurements allows the study of solar energetic particle (SEP) events over a wide longitudinal range with minimal radial gradient effects. This contribution provides an overview of recent results obtained by the STEREO/IMPACT team in combination with observations by the ACE and SOHO spacecraft. We focus especially on multi-spacecraft investigations of SEP events. The large longitudinal spread of electron and 3He-rich events as well as unusual anisotropies will be presented and discussed.

STEREO in-situ data analysis

NASA Astrophysics Data System (ADS)

Schroeder, P.; Luhmann, J.; Davis, A.; Russell, C.

STEREO s IMPACT In-situ Measurements of Particles and CME Transients investigation provides the first opportunity for long duration detailed observations of 1 AU magnetic field structures plasma and suprathermal electrons and energetic particles at points bracketing Earth s heliospheric location The PLASTIC instrument will make plasma ion composition measurements completing STEREO s comprehensive in-situ perspective Stereoscopic 3D information from the STEREO SECCHI imagers and SWAVES radio experiment will make it possible to use both multipoint and quadrature studies to connect interplanetary Coronal Mass Ejections ICME and solar wind structures to CMEs and coronal holes observed at the Sun The uniqueness of the STEREO mission requires novel data analysis tools and techniques to take advantage of the mission s full scientific potential An interactive browser with the ability to create publication-quality plots is being developed which will integrate STEREO s in-situ data with data from a variety of other missions including WIND and ACE Also an application program interface API will be provided allowing users to create custom software that ties directly into STEREO s data set The API will allow for more advanced forms of data mining than currently available through most data web services A variety of data access techniques and the development of cross-spacecraft data analysis tools will allow the larger scientific community to combine STEREO s unique in-situ data with those of other missions particularly the L1 missions and therefore to maximize

Spine micromorphology of normal and hyperhydric Mammillaria gracilis Pfeiff. (Cactaceae) shoots.

PubMed

Peharec, P; Posilović, H; Balen, B; Krsnik-Rasol, M

2010-07-01

Artificial conditions of tissue culture affect growth and physiology of crassulacean acid metabolism plants which often results in formation of hyperhydric shoots. In in vitro conditions Mammillaria gracilis Pfeiff. (Cactaceae) growth switches from organized to unorganized way, producing a habituated organogenic callus which simultaneously regenerates morphologically normal as well as altered hyperhydric shoots. In this study, influence of tissue culture conditions on morphology of cactus spines of normal and hyperhydric shoots was investigated. Spines of pot-grown Mammillaria plants and of in vitro regenerated shoots were examined with stereo microscope and scanning electron microscope. The pot-grown plants had 16-17 spines per areole. In vitro grown normal shoots, even though they kept typical shoot morphology, had lower number of spines (11-12) and altered spine morphology. This difference was even more pronounced in spine number (six to seven) and morphology of the hyperhydric shoots. Scanning electron microscopy analysis revealed remarkable differences in micromorphology of spine surface between pot-grown and in vitro grown shoots. Spines of in vitro grown normal shoots showed numerous long trichomes, which were more elongated on spines of the hyperhydric shoots; the corresponding structures on spine surface of pot-grown plants were noticed only as small protrusions. Scanning electron microscopy morphometric studies showed that the spines of pot-grown plants were significantly longer compared to the spines of shoots grown in tissue culture. Moreover, transverse section shape varies from elliptical in pot-grown plants to circular in normal and hyperhydric shoots grown in vitro. Cluster and correspondence analyses performed on the scanning electron microscope obtained results suggest great variability among spines of pot-grown plants. Spines of in vitro grown normal and hyperhydric shoots showed low level of morphological variation among themselves despite the significant difference in shoot morphology.

Stereo imaging and cytocompatibility of a model dental implant surface formed by direct laser fabrication.

PubMed

Mangano, Carlo; Raspanti, Mario; Traini, Tonino; Piattelli, Adriano; Sammons, Rachel

2009-03-01

Direct laser fabrication (DLF) allows solids with complex geometry to be produced by sintering metal powder particles in a focused laser beam. In this study, 10 Ti6Al4V alloy model dental root implants were obtained by DLF, and surface characterization was carried out using stereo scanning electron microscopy to produce 3D reconstructions. The surfaces were extremely irregular, with approximately 100 microm deep, narrow intercommunicating crevices, shallow depressions and deep, rounded pits of widely variable shape and size, showing ample scope for interlocking with the host bone. Roughness parameters were as follows: R(t), 360.8 microm; R(z), 358.4 microm; R(a), 67.4 microm; and R(q), 78.0 microm. Disc specimens produced by DLF with an identically prepared surface were used for biocompatibility studies with rat calvarial osteoblasts: After 9 days, cells had attached and spread on the DLF surface, spanning across the crevices, and voids. Cell density was similar to that on a commercial rough microtextured surface but lower than on commercial smooth machined and smooth-textured grit-blasted, acid-etched surfaces. Human fibrin clot extension on the DLF surface was slightly improved by inorganic acid etching to increase the microroughness. With further refinements, DLF could be an economical means of manufacturing implants from titanium alloys. (c) 2008 Wiley Periodicals, Inc.

A Real-Time Imaging System for Stereo Atomic Microscopy at SPring-8's BL25SU

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matsushita, Tomohiro; Guo, Fang Zhun; Muro, Takayuki

2007-01-19

We have developed a real-time photoelectron angular distribution (PEAD) and Auger-electron angular distribution (AEAD) imaging system at SPring-8 BL25SU, Japan. In addition, a real-time imaging system for circular dichroism (CD) studies of PEAD/AEAD has been newly developed. Two PEAD images recorded with left- and right-circularly polarized light can be regarded as a stereo image of the atomic arrangement. A two-dimensional display type mirror analyzer (DIANA) has been installed at the beamline, making it possible to record PEAD/AEAD patterns with an acceptance angle of {+-}60 deg. in real-time. The twin-helical undulators at BL25SU enable helicity switching of the circularly polarized lightmore » at 10Hz, 1Hz or 0.1Hz. In order to realize real-time measurements of the CD of the PEAD/AEAD, the CCD camera must be synchronized to the switching frequency. The VME computer that controls the ID is connected to the measurement computer with two BNC cables, and the helicity information is sent using TTL signals. For maximum flexibility, rather than using a hardware shutter synchronizing with the TTL signal we have developed software to synchronize the CCD shutter with the TTL signal. We have succeeded in synchronizing the CCD camera in both the 1Hz and 0.1Hz modes.« less

Catalogue of {>} 55 MeV Wide-longitude Solar Proton Events Observed by SOHO, ACE, and the STEREOs at {≈} 1 AU During 2009 - 2016

NASA Astrophysics Data System (ADS)

Paassilta, Miikka; Papaioannou, Athanasios; Dresing, Nina; Vainio, Rami; Valtonen, Eino; Heber, Bernd

2018-04-01

Based on energetic particle observations made at {≈} 1 AU, we present a catalogue of 46 wide-longitude ({>} 45°) solar energetic particle (SEP) events detected at multiple locations during 2009 - 2016. The particle kinetic energies of interest were chosen as {>} 55 MeV for protons and 0.18 - 0.31 MeV for electrons. We make use of proton data from the Solar and Heliospheric Observatory/Energetic and Relativistic Nuclei and Electron Experiment (SOHO/ERNE) and the Solar Terrestrial Relations Observatory/High Energy Telescopes (STEREO/HET), together with electron data from the Advanced Composition Explorer/Electron, Proton, and Alpha Monitor (ACE/EPAM) and the STEREO/ Solar Electron and Proton Telescopes (SEPT). We consider soft X-ray data from the Geostationary Operational Environmental Satellites (GOES) and coronal mass ejection (CME) observations made with the SOHO/ Large Angle and Spectrometric Coronagraph (LASCO) and STEREO/ Coronagraphs 1 and 2 (COR1, COR2) to establish the probable associations between SEP events and the related solar phenomena. Event onset times and peak intensities are determined; velocity dispersion analysis (VDA) and time-shifting analysis (TSA) are performed for protons; TSA is performed for electrons. In our event sample, there is a tendency for the highest peak intensities to occur when the observer is magnetically connected to solar regions west of the flare. Our estimates for the mean event width, derived as the standard deviation of a Gaussian curve modelling the SEP intensities (protons {≈} 44°, electrons {≈} 50°), largely agree with previous results for lower-energy SEPs. SEP release times with respect to event flares, as well as the event rise times, show no simple dependence on the observer's connection angle, suggesting that the source region extent and dominant particle acceleration and transport mechanisms are important in defining these characteristics of an event. There is no marked difference between the speed distributions of the CMEs related to wide events and the CMEs related to all near-Earth SEP events of similar energy range from the same time period.

Study of a stereo electro-optical tracker system for the measurement of model deformations at the national transonic facility

NASA Technical Reports Server (NTRS)

Hertel, R. J.

1979-01-01

An electro-optical method to measure the aeroelastic deformations of wind tunnel models is examined. The multitarget tracking performance of one of the two electronic cameras comprising the stereo pair is modeled and measured. The properties of the targets at the model, the camera optics, target illumination, number of targets, acquisition time, target velocities, and tracker performance are considered. The electronic camera system is shown to be capable of locating, measuring, and following the positions of 5 to 50 targets attached to the model at measuring rates up to 5000 targets per second.

The High Energy Telescopes for the STEREO Mission

NASA Astrophysics Data System (ADS)

von Rosenvinge, T.; Cummings, A.; Cohen, C.; Leske, R.; Mewaldt, R.; Stone, E.; Wiedenbeck, M.

2007-05-01

The High Energy Telescopes (HETs) described in this paper are part of the IMPACT investigation for the STEREO mission (Principal Investigator: Janet Luhmann, University of California at Berkeley). The two STEREO spacecraft were launched from Cape Canaveral, FL on October 25, 2006. High energy electrons (~ 0.7 -6 MeV) and nuclei from hydrogen to iron (~ 13 - 200 MeV/nucleon) are detected by the HETs, one on each spacecraft. The HET design, the associated electronics, and the on-board software are described in some detail. Observations from one pass through the Earth's magnetosphere and from four X-class solar events in December, 2006 are presented to illustrate the capabilities of the HETs. The event of December 13th was a Ground Level Event. We will compare the elemental composition of this event with that of a previous Ground Level Event on January 20, 2005. This work was supported by NASA (at Caltech and JPL under contract NAS5-00133).

The Dynamic Photometric Stereo Method Using a Multi-Tap CMOS Image Sensor.

PubMed

Yoda, Takuya; Nagahara, Hajime; Taniguchi, Rin-Ichiro; Kagawa, Keiichiro; Yasutomi, Keita; Kawahito, Shoji

2018-03-05

The photometric stereo method enables estimation of surface normals from images that have been captured using different but known lighting directions. The classical photometric stereo method requires at least three images to determine the normals in a given scene. However, this method cannot be applied to dynamic scenes because it is assumed that the scene remains static while the required images are captured. In this work, we present a dynamic photometric stereo method for estimation of the surface normals in a dynamic scene. We use a multi-tap complementary metal-oxide-semiconductor (CMOS) image sensor to capture the input images required for the proposed photometric stereo method. This image sensor can divide the electrons from the photodiode from a single pixel into the different taps of the exposures and can thus capture multiple images under different lighting conditions with almost identical timing. We implemented a camera lighting system and created a software application to enable estimation of the normal map in real time. We also evaluated the accuracy of the estimated surface normals and demonstrated that our proposed method can estimate the surface normals of dynamic scenes.

Three-dimensional reconstruction of highly complex microscopic samples using scanning electron microscopy and optical flow estimation.

PubMed

Baghaie, Ahmadreza; Pahlavan Tafti, Ahmad; Owen, Heather A; D'Souza, Roshan M; Yu, Zeyun

2017-01-01

Scanning Electron Microscope (SEM) as one of the major research and industrial equipment for imaging of micro-scale samples and surfaces has gained extensive attention from its emerge. However, the acquired micrographs still remain two-dimensional (2D). In the current work a novel and highly accurate approach is proposed to recover the hidden third-dimension by use of multi-view image acquisition of the microscopic samples combined with pre/post-processing steps including sparse feature-based stereo rectification, nonlocal-based optical flow estimation for dense matching and finally depth estimation. Employing the proposed approach, three-dimensional (3D) reconstructions of highly complex microscopic samples were achieved to facilitate the interpretation of topology and geometry of surface/shape attributes of the samples. As a byproduct of the proposed approach, high-definition 3D printed models of the samples can be generated as a tangible means of physical understanding. Extensive comparisons with the state-of-the-art reveal the strength and superiority of the proposed method in uncovering the details of the highly complex microscopic samples.

Inexpensive driver for stereo videogame glasses

NASA Astrophysics Data System (ADS)

Pique, Michael; Coogan, Anthony

1990-09-01

We have adapted home videogame glasses from Sega as workstation stereo viewers. A small (4x7x9 cm.) box of electronics receives sync signals in parallel with the monitor (either separate ROB-Sync or composite video) and drives the glasses.The view is dimmer than with costlier shutters, there is more ghosting, and the user is feuered by the wires. But the glasses are so much cheaper than the full-screen shutters (250 instead of about 10 000) that it is practical to provide the benefits of stereo to many more workstation users. We are using them with Sun TAAC-1 workstations; the interlaced video can also be recorded on ordinary NTSC videotape and played on television monitors.

Large Amplitude Whistlers in the Magnetosphere Observed with Wind-Waves

NASA Technical Reports Server (NTRS)

Kellogg, P. J.; Cattell, C. A.; Goetz, K.; Monson, S. J.; Wilson, L. B., III

2011-01-01

We describe the results of a statistical survey of Wind-Waves data motivated by the recent STEREO/Waves discovery of large-amplitude whistlers in the inner magnetosphere. Although Wind was primarily intended to monitor the solar wind, the spacecraft spent 47 h inside 5 R(sub E) and 431 h inside 10 R(sub E) during the 8 years (1994-2002) that it orbited the Earth. Five episodes were found when whistlers had amplitudes comparable to those of Cattell et al. (2008), i.e., electric fields of 100 m V/m or greater. The whistlers usually occurred near the plasmapause. The observations are generally consistent with the whistlers observed by STEREO. In contrast with STEREO, Wind-Waves had a search coil, so magnetic measurements are available, enabling determination of the wave vector without a model. Eleven whistler events with useable magnetic measurements were found. The wave vectors of these are distributed around the magnetic field direction with angles from 4 to 48deg. Approximations to observed electron distribution functions show a Kennel-Petschek instability which, however, does not seem to produce the observed whistlers. One Wind episode was sampled at 120,000 samples/s, and these events showed a signature that is interpreted as trapping of electrons in the electrostatic potential of an oblique whistler. Similar waveforms are found in the STEREO data. In addition to the whistler waves, large amplitude, short duration solitary waves (up to 100 mV/m), presumed to be electron holes, occur in these passes, primarily on plasma sheet field lines mapping to the auroral zone.

Epifluorescence and stereomicroscopy of trichomes associated with resistant and susceptible host plant genotypes of the Asian citrus psyllid (Hemiptera: Liviidae)

USDA-ARS?s Scientific Manuscript database

Epifluorescence, light and stereo-microscopy were used to characterize foliar trichomes associated with young flush leaves and stems of six plant genotypes that are hosts of the Asian citrus psyllid, Diaphorina citri, vector of the economically important citrus greening disease. These genotypes incl...

[Fractographic analysis of clinically failed anterior all ceramic crowns].

PubMed

DU, Qian; Zhou, Min-bo; Zhang, Xin-ping; Zhao, Ke

2012-04-01

To identify the site of crack initiation and propagation path of clinically failed all ceramic crowns by fractographic analysis. Three clinically failed anterior IPS Empress II crowns and two anterior In-Ceram alumina crowns were retrieved. Fracture surfaces were examined using both optical stereo and scanning electron microscopy. Fractographic theory and fracture mechanics principles were applied to disclose the damage characteristics and fracture mode. All the crowns failed by cohesive failure within the veneer on the labial surface. Critical crack originated at the incisal contact area and propagated gingivally. Porosity was found within the veneer because of slurry preparation and the sintering of veneer powder. Cohesive failure within the veneer is the main failure mode of all ceramic crown. Veneer becomes vulnerable when flaws are present. To reduce the chances of chipping, multi-point occlusal contacts are recommended, and layering and sintering technique of veneering layer should also be improved.

Identification of modes of fracture in a 2618-T6 aluminum alloy using stereophotogrammetry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Salas Zamarripa, A., E-mail: a.salaszamarripa@gmail.com; Pinna, C.; Brown, M.W.

2011-12-15

The identification and the development of a quantification technique of the modes of fracture in fatigue fracture surfaces of a 2618-T6 aluminum alloy were developed during this research. Fatigue tests at room and high temperature (230 Degree-Sign C) were carried out to be able to compare the microscopic fractographic features developed by this material under these testing conditions. The overall observations by scanning electron microscopy (SEM) of the fracture surfaces showed a mixture of transgranular and ductile intergranular fracture. The ductile intergranular fracture contribution appears to be more significant at room temperature than at 230 Degree-Sign C. A quantitative methodologymore » was developed to identify and to measure the contribution of these microscopic fractographic features. The technique consisted of a combination of stereophotogrammetry and image analysis. Stereo-pairs were randomly taken along the crack paths and were then analyzed using the profile module of MeX software. The analysis involved the 3-D surface reconstruction, the trace of primary profile lines in both vertical and horizontal directions within the stereo-pair area, the measurements of the contribution of the modes of fracture in each profile, and finally, the calculation of the average contribution in each stereo-pair. The technique results confirmed a higher contribution of ductile intergranular fracture at room temperature than at 230 Degree-Sign C. Moreover, there was no indication of a direct relationship between this contribution and the strain amplitudes range applied during the fatigue testing. - Highlights: Black-Right-Pointing-Pointer Stereophotogrammetry and image analysis as a measuring tool of modes of fracture in fatigue fracture surfaces. Black-Right-Pointing-Pointer A mixture of ductile intergranular and transgranular fracture was identified at room temperature and 230 Degree-Sign C testing. Black-Right-Pointing-Pointer Development of a quantitative methodology to obtain the percentage of modes of fracture within the fracture surface.« less

Quantitative fractography by digital image processing: NIH Image macro tools for stereo pair analysis and 3-D reconstruction.

PubMed

Hein, L R

2001-10-01

A set of NIH Image macro programs was developed to make qualitative and quantitative analyses from digital stereo pictures produced by scanning electron microscopes. These tools were designed for image alignment, anaglyph representation, animation, reconstruction of true elevation surfaces, reconstruction of elevation profiles, true-scale elevation mapping and, for the quantitative approach, surface area and roughness calculations. Limitations on time processing, scanning techniques and programming concepts are also discussed.

System for clinical photometric stereo endoscopy

NASA Astrophysics Data System (ADS)

Durr, Nicholas J.; González, Germán.; Lim, Daryl; Traverso, Giovanni; Nishioka, Norman S.; Vakoc, Benjamin J.; Parot, Vicente

2014-02-01

Photometric stereo endoscopy is a technique that captures information about the high-spatial-frequency topography of the field of view simultaneously with a conventional color image. Here we describe a system that will enable photometric stereo endoscopy to be clinically evaluated in the large intestine of human patients. The clinical photometric stereo endoscopy system consists of a commercial gastroscope, a commercial video processor, an image capturing and processing unit, custom synchronization electronics, white light LEDs, a set of four fibers with diffusing tips, and an alignment cap. The custom pieces that come into contact with the patient are composed of biocompatible materials that can be sterilized before use. The components can then be assembled in the endoscopy suite before use. The resulting endoscope has the same outer diameter as a conventional colonoscope (14 mm), plugs into a commercial video processor, captures topography and color images at 15 Hz, and displays the conventional color image to the gastroenterologist in real-time. We show that this system can capture a color and topographical video in a tubular colon phantom, demonstrating robustness to complex geometries and motion. The reported system is suitable for in vivo evaluation of photometric stereo endoscopy in the human large intestine.

The Dynamic Photometric Stereo Method Using a Multi-Tap CMOS Image Sensor †

PubMed Central

Yoda, Takuya; Nagahara, Hajime; Taniguchi, Rin-ichiro; Kagawa, Keiichiro; Yasutomi, Keita; Kawahito, Shoji

2018-01-01

The photometric stereo method enables estimation of surface normals from images that have been captured using different but known lighting directions. The classical photometric stereo method requires at least three images to determine the normals in a given scene. However, this method cannot be applied to dynamic scenes because it is assumed that the scene remains static while the required images are captured. In this work, we present a dynamic photometric stereo method for estimation of the surface normals in a dynamic scene. We use a multi-tap complementary metal-oxide-semiconductor (CMOS) image sensor to capture the input images required for the proposed photometric stereo method. This image sensor can divide the electrons from the photodiode from a single pixel into the different taps of the exposures and can thus capture multiple images under different lighting conditions with almost identical timing. We implemented a camera lighting system and created a software application to enable estimation of the normal map in real time. We also evaluated the accuracy of the estimated surface normals and demonstrated that our proposed method can estimate the surface normals of dynamic scenes. PMID:29510599

Multi-spacecraft observations of recurrent {sup 3}He-rich solar energetic particles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bučík, R.; Innes, D. E.; Mall, U.

2014-05-01

We study the origin of {sup 3}He-rich solar energetic particles (<1 MeV nucleon{sup –1}) that are observed consecutively on STEREO-B, Advanced Composition Explorer (ACE), and STEREO-A spacecraft when they are separated in heliolongitude by more than 90°. The {sup 3}He-rich period on STEREO-B and STEREO-A commences on 2011 July 1 and 2011 July 16, respectively. The ACE {sup 3}He-rich period consists of two sub-events starting on 2011 July 7 and 2011 July 9. We associate the STEREO-B July 1 and ACE July 7 {sup 3}He-rich events with the same sizeable active region (AR) producing X-ray flares accompanied by prompt electronmore » events, when it was near the west solar limb as seen from the respective spacecraft. The ACE July 9 and STEREO-A July 16 events were dispersionless with enormous {sup 3}He enrichment, lacking solar energetic electrons and occurring in corotating interaction regions. We associate these events with a small, recently emerged AR near the border of a low-latitude coronal hole that produced numerous jet-like emissions temporally correlated with type III radio bursts. For the first time we present observations of (1) solar regions with long-lasting conditions for {sup 3}He acceleration and (2) solar energetic {sup 3}He that is temporarily confined/re-accelerated in interplanetary space.« less

Langmuir waveforms at interplanetary shocks: STEREO statistical analysis

NASA Astrophysics Data System (ADS)

Briand, C.

2016-12-01

Wave-particle interactions and particle acceleration are the two main processes allowing energy dissipation at non collisional shocks. Ion acceleration has been deeply studied for many years, also for their central role in the shock front reformation. Electron dynamics is also important in the shock dynamics through the instabilities they can generate which may impact the ion dynamics.Particle measurements can be efficiently completed by wave measurements to determine the characteristics of the electron beams and study the turbulence of the medium. Electric waveforms obtained from the S/WAVES instrument of the STEREO mission between 2007 to 2014 are analyzed. Thus, clear signature of Langmuir waves are observed on 41 interplanetary shocks. These data enable a statistical analysis and to deduce some characteristics of the electron dynamics on different shocks sources (SIR or ICME) and types (quasi-perpendicular or quasi-parallel). The conversion process between electrostatic to electromagnetic waves has also been tested in several cases.

Profiling with the electron microscope.

NASA Technical Reports Server (NTRS)

Vedder, J. F.; Lem, H. Y.

1972-01-01

Discussion of a profiling technique using a scanning electron microscope for obtaining depth information on a single micrograph of a small specimen. A stationary electron beam is used to form a series of contamination spots in a line across the specimen. Micrographs obtained by this technique are useful as a means of projection and display where stereo viewers are not practical.

The molecular shape and the field similarities as criteria to interpret SAR studies for fragment-based design of platinum(IV) anticancer agents. Correlation of physicochemical properties with cytotoxicity.

PubMed

Lorenzo, Julia; Montaña, Ángel M

2016-09-01

Molecular shape similarity and field similarity have been used to interpret, in a qualitative way, the structure-activity relationships in a selected series of platinum(IV) complexes with anticancer activity. MM and QM calculations have been used to estimate the electron density, electrostatic potential maps, partial charges, dipolar moments and other parameters to correlate the stereo-electronic properties with the differential biological activity of complexes. Extended Electron Distribution (XED) field similarity has been also evaluated for the free 1,4-diamino carrier ligands, in a fragment-based drug design approach, comparing Connolly solvent excluded surface, hydrophobicity field surface, Van der Waals field surface, nucleophilicity field surface, electrophilicity field surface and the extended electron-distribution maxima field points. A consistency has been found when comparing the stereo-electronic properties of the studied series of platinum(IV) complexes and/or the free ligands evaluated and their in vitro anticancer activity. Copyright © 2016 Elsevier Inc. All rights reserved.

Ultrastructural networks in growth cones and neurites of cultured central nervous system neurons.

PubMed

Tsui, H C; Ris, H; Klein, W L

1983-09-01

We have examined growth cones and neurites of cultured central nervous system neurons by high-voltage electron microscopy. Embryonic chicken retina cells were cultured on polylysine-treated and Formvar-coated gold grids for 2-6 days, fixed, and critical point dried. Growth cones and neurites were examined as unembedded whole mounts. Three-dimensional images from stereo-pair electron micrographs of these regions showed a high degree of ultrastructural articulation, with distinct, non-tapering filaments (5-9 nm in diameter) joining both cytoskeletal and membranous components. In the central regions of growth cones, interconnected structures included microtubules, large membranous sacs (up to 400 nm), and irregular vesicles (25-75 nm). A denser filamentous network was prevalent at the edges of growth cones. This network, which frequently adjoined the surface membrane, linked vesicles of uniform size (35-40 nm). Such vesicles often were seen densely packed in growth cone protrusions that were about the size of small synaptic boutons. Prevalent structural interconnections within growth cones conceivably could play a logistic role in specific membrane assembly, intracellular transport, endocytosis, and secretion. Because such processes are not unique to growth cones, the extensive linkages we have observed may have implications for cytoplasmic structure in general.

The research on calibration methods of dual-CCD laser three-dimensional human face scanning system

NASA Astrophysics Data System (ADS)

Wang, Jinjiang; Chang, Tianyu; Ge, Baozhen; Tian, Qingguo; Yang, Fengting; Shi, Shendong

2013-09-01

In this paper, on the basis of considering the performance advantages of two-step method, we combines the stereo matching of binocular stereo vision with active laser scanning to calibrate the system. Above all, we select a reference camera coordinate system as the world coordinate system and unity the coordinates of two CCD cameras. And then obtain the new perspective projection matrix (PPM) of each camera after the epipolar rectification. By those, the corresponding epipolar equation of two cameras can be defined. So by utilizing the trigonometric parallax method, we can measure the space point position after distortion correction and achieve stereo matching calibration between two image points. Experiments verify that this method can improve accuracy and system stability is guaranteed. The stereo matching calibration has a simple process with low-cost, and simplifies regular maintenance work. It can acquire 3D coordinates only by planar checkerboard calibration without the need of designing specific standard target or using electronic theodolite. It is found that during the experiment two-step calibration error and lens distortion lead to the stratification of point cloud data. The proposed calibration method which combining active line laser scanning and binocular stereo vision has the both advantages of them. It has more flexible applicability. Theory analysis and experiment shows the method is reasonable.

Comparative Analysis of Cutting Efficiency and Surface Maintenance Between Different Types of Implant Drills: An In Vitro Study.

PubMed

Hochscheidt, Celso João; Shimizu, Roberto Hideo; Andrighetto, Augusto Ricardo; Pierezan, Rodrigo; Thomé, Geninho; Salatti, Rafael

2017-10-01

This study evaluated cutting efficiency (CE) and linear wear of dental implant drills after 450 standardized osteotomies on bovine ribs. Diamond-like carbon-coated steel drills (SG), acid-treated steel drills (EG), and ceramic drills (ZG) were divided into 6 subgroups according to the number of uses. A robot-controlled program performed systematic instrumentation, timing, axial loading, and managed feed rate. CE was recorded in a polyurethane resin blank and end wear (VBBmax) was analyzed under stereo microscopy. After osteotomies in beef ribs, CE for the Ø2.0-mm drill decreased 10.2% in SG and 10.9% in ZG; for the Ø3.0-mm drill, CE decreased 30.6% in SG, 8.5% in ZG, and improved in EG. The greatest wear occurred in Ø2.0-mm drills; ZG drills (Ø3.0 mm) exhibited only edge frittering, as confirmed on scanning electron microscopy. After 50 exposures to mechanical loads, steel and ceramic drills lost CE. Whereas cutting and thermal performance improved in experimental drills, the Ø2.0-mm drill exhibited the most signs of wear proportional to use. These findings suggest that, with the methodology employed, the life of these drills exceeds 50 osteotomies.

Structured light optical microscopy for three-dimensional reconstruction of technical surfaces

NASA Astrophysics Data System (ADS)

Kettel, Johannes; Reinecke, Holger; Müller, Claas

2016-04-01

In microsystems technology quality control of micro structured surfaces with different surface properties is playing an ever more important role. The process of quality control incorporates three-dimensional (3D) reconstruction of specularand diffusive reflecting technical surfaces. Due to the demand on high measurement accuracy and data acquisition rates, structured light optical microscopy has become a valuable solution to solve this problem providing high vertical and lateral resolution. However, 3D reconstruction of specular reflecting technical surfaces still remains a challenge to optical measurement principles. In this paper we present a measurement principle based on structured light optical microscopy which enables 3D reconstruction of specular- and diffusive reflecting technical surfaces. It is realized using two light paths of a stereo microscope equipped with different magnification levels. The right optical path of the stereo microscope is used to project structured light onto the object surface. The left optical path is used to capture the structured illuminated object surface with a camera. Structured light patterns are generated by a Digital Light Processing (DLP) device in combination with a high power Light Emitting Diode (LED). Structured light patterns are realized as a matrix of discrete light spots to illuminate defined areas on the object surface. The introduced measurement principle is based on multiple and parallel processed point measurements. Analysis of the measured Point Spread Function (PSF) by pattern recognition and model fitting algorithms enables the precise calculation of 3D coordinates. Using exemplary technical surfaces we demonstrate the successful application of our measurement principle.

New Data Products and Tools from STEREO's IMPACT Investigation

NASA Astrophysics Data System (ADS)

Schroeder, P. C.; Luhmann, J. G.; Li, Y.; Huttunen, E.; Toy, V.; Russell, C. T.; Davis, A.

2008-05-01

STEREO's IMPACT (In-situ Measurements of Particles and CME Transients) investigation provides the first opportunity for long duration, detailed observations of 1 AU magnetic field structures, plasma and suprathermal electrons, and energetic particles at points bracketing Earth's heliospheric location. The IMPACT team has developed both human-friendly web portals and API's (application program interfaces) which provide straight- foward data access to scientists and the Virtual Observatories. Several new data products and web features have been developed including the release of more and higher-level data products, a library of TPLOT-based IDL data analysis routines, and web sites devoted to event analysis and inter-mission and inter-disciplinary collaboration. A web browser integrating STEREO and L1 in situ data with imaging and models provides a powerful venue for exploring the Sun-Earth connection. These latest releases enable the larger scientific community to use STEREO's unique in-situ data in novel ways along side "third eye" data sets from Wind, ACE and other heliospheric missions contributing to a better understanding of three dimensional structures in the solar wind.

Investigation of Different Colloidal Porous Silicon Solutions and Their Composite Solid Matrix Rods by Optical Techniques

NASA Astrophysics Data System (ADS)

Khan, M. Naziruddin; Aldalbahi, Ali; Almohammedi, Abdullah

2018-03-01

Colloidal porous silicon (PSi) in different solvents was synthesized by simple chemical etching. Colloidal solutions were then prepared using different quantities of silicon wafer pieces (Pcs) and chloroplatinic (Pt) acid in catalyst solution. The effect on the properties of the colloidal solutions and composite rods were investigated using various optical characterization techniques. Absorption and photoluminescence (PL) intensity of the colloidal PSi solutions are observed to depend on the quantity of wafer Pcs, the Pt-solution, and the porosity formation on the wafer surface. The morphological structure of the PSi in a solvent and the solid-rod environments were studied using field-emission scanning electron microscopy (FE-SEM) and were observed to have different structures. A mono-oriented structure of PSi exists in tetrahydrofuran, which has stereo orientation in dioxane and dimethylsulfoxide (approximately 5-8 nm as confirmed using high resolution transmission electron microscopy). Subsequently, some colloidal PSi solutions were directly embedded in three types of sol-gel-based matrices, silica, ormosils (or organically modified silica) and polymer, which easily generated solid rods. Spontaneous emission (SE) of the PSi solutions and their composite rods were examined using a high power picosecond 355 nm laser source. The emitted PL and SE signals of the colloidal PSi solutions were dependent on the Pt volume, nature of the solvent, quantity of Si wafer piece, and pumping energy. The response of SE signals from the PSi composites rods is an interesting phenomenon, and such nanocomposites may be used for future research on light amplification.

Investigation of Different Colloidal Porous Silicon Solutions and Their Composite Solid Matrix Rods by Optical Techniques

NASA Astrophysics Data System (ADS)

Khan, M. Naziruddin; Aldalbahi, Ali; Almohammedi, Abdullah

2018-07-01

Colloidal porous silicon (PSi) in different solvents was synthesized by simple chemical etching. Colloidal solutions were then prepared using different quantities of silicon wafer pieces (Pcs) and chloroplatinic (Pt) acid in catalyst solution. The effect on the properties of the colloidal solutions and composite rods were investigated using various optical characterization techniques. Absorption and photoluminescence (PL) intensity of the colloidal PSi solutions are observed to depend on the quantity of wafer Pcs, the Pt-solution, and the porosity formation on the wafer surface. The morphological structure of the PSi in a solvent and the solid-rod environments were studied using field-emission scanning electron microscopy (FE-SEM) and were observed to have different structures. A mono-oriented structure of PSi exists in tetrahydrofuran, which has stereo orientation in dioxane and dimethylsulfoxide (approximately 5-8 nm as confirmed using high resolution transmission electron microscopy). Subsequently, some colloidal PSi solutions were directly embedded in three types of sol-gel-based matrices, silica, ormosils (or organically modified silica) and polymer, which easily generated solid rods. Spontaneous emission (SE) of the PSi solutions and their composite rods were examined using a high power picosecond 355 nm laser source. The emitted PL and SE signals of the colloidal PSi solutions were dependent on the Pt volume, nature of the solvent, quantity of Si wafer piece, and pumping energy. The response of SE signals from the PSi composites rods is an interesting phenomenon, and such nanocomposites may be used for future research on light amplification.

STEREO's Extreme UltraViolet Imager (EUVI)

NASA Technical Reports Server (NTRS)

2007-01-01

At a pixel resolution of 2048x2048, the STEREO EUVI instrument provides views of the Sun in ultraviolet light that rivals the full-disk views of SOHO/EIT. This image is through the 171 Angstrom (ultraviolet) filter which is characteristic of iron ions (missing eight and nine electrons) at 1 million degrees. There is a short data gap in the latter half of the movie that creates a freeze and then jump in the data view. This is a movie of the Sun in 171 Angstrom ultraviolet light. The time frame is late January, 2007

Solid state electro-optic color filter and iris

NASA Technical Reports Server (NTRS)

1975-01-01

A pair of solid state electro-optic filters (SSEF) in a binocular holder were designed and fabricated for evaluation of field sequential stereo TV applications. The electronic circuitry for use with the stereo goggles was designed and fabricated, requiring only an external video input. A polarizing screen suitable for attachment to various size TV monitors for use in conjunction with the stereo goggles was designed and fabricated. An improved engineering model 2 filter was fabricated using the bonded holder technique developed previously and integrated to a GCTA color TV camera. An engineering model color filter was fabricated and assembled using PLZT control elements. In addition, a ruggedized holder assembly was designed, fabricated and tested. This assembly provides electrical contacts, high voltage protection, and support for the fragile PLZT disk, and also permits mounting and optical alignment of the associated polarizers.

Chains of magnetite crystals in the meteorite ALH84001: Evidence of biological origin

PubMed Central

Friedmann, E. Imre; Wierzchos, Jacek; Ascaso, Carmen; Winklhofer, Michael

2001-01-01

The presence of magnetite crystal chains, considered missing evidence for the biological origin of magnetite in ALH84001 [Thomas-Keprta, K. L., Bazylinski, D. A., Kirschvink, J. L., Clemett, S. J., McKay, D. S., Wentworth, S. J., Vali, H., Gibson, E. K., Jr., & Romanek, C. S. (2000) Geochim. Cosmochim. Acta 64, 4049–4081], is demonstrated by high-power stereo backscattered scanning electron microscopy. Five characteristics of such chains (uniform crystal size and shape within chains, gaps between crystals, orientation of elongated crystals along the chain axis, flexibility of chains, and a halo that is a possible remnant of a membrane around chains), observed or inferred to be present in magnetotactic bacteria but incompatible with a nonbiological origin, are shown to be present. Although it is unlikely that magnetotactic bacteria were ever alive in ALH84001, decomposed remains of such organisms could have been deposited in cracks in the rock while it was still on the surface on Mars. PMID:11226212

LPG sensing characteristics of electrospray deposited SnO2 nanoparticles

NASA Astrophysics Data System (ADS)

Gürbüz, Mevlüt; Günkaya, Göktuğ; Doğan, Aydın

2014-11-01

In this study, SnO2 films were fabricated on conductive substrate such as aluminum and platinum coated alumina using electro-spray deposition (ESD) method for gas sensor applications. Solution flow rate, coating time, substrate-nozzle distance and solid/alcohol ratio were studied to optimize SnO2 film structure. The morphology of the deposited films was characterized by stereo and scanning electron microscopy (SEM). The gas sensing properties of tin oxide films were investigated using liquid petroleum gas (LPG) for various lower explosive limit (LEL). The results obtained from microscopic analyses show that optimum SnO2 films were evaluated at flow rate of 0.05 ml/min, at distance of 6 cm, for 10 min deposition time, for 20 gSnO2/Lethanol ratio and at 7 kV DC electric field. By the results obtained from the gas sensing behavior, the sensitivity of the films was increased with operating temperature. The films showed better sensitivity for 20 LEL LPG concentration at 450 °C operating temperature.

Jovian decametric radiation seen from Juno, Cassini, STEREO A, WIND, and Earth-based radio observatories

NASA Astrophysics Data System (ADS)

Imai, M.; Kurth, W. S.; Hospodarsky, G. B.; Bolton, S. J.; Connerney, J. E. P.; Levin, S. M.; Lecacheux, A.; Lamy, L.; Zarka, P.; Clarke, T. E.; Higgins, C. A.

2017-09-01

Jupiter's decametric (DAM) radiation is generated very close to the local gyrofrequency by the electron cyclotron maser instability (CMI). The first two-point common detections of Jovian DAM radiation were made using the Voyager spacecraft and ground-based radio observatories in early 1979, but, due to geometrical constraints and limited flyby duration, a full understanding of the latitudinal beaming of Jovian DAM radiation remains elusive. The stereoscopic DAM radiation viewed from Juno, Cassini, STEREO A, WIND, and Earth-based radio observatories provides a unique opportunity to analyze the CMI emission mechanism and beaming properties.

Robust surface reconstruction by design-guided SEM photometric stereo

NASA Astrophysics Data System (ADS)

Miyamoto, Atsushi; Matsuse, Hiroki; Koutaki, Gou

2017-04-01

We present a novel approach that addresses the blind reconstruction problem in scanning electron microscope (SEM) photometric stereo for complicated semiconductor patterns to be measured. In our previous work, we developed a bootstrapping de-shadowing and self-calibration (BDS) method, which automatically calibrates the parameter of the gradient measurement formulas and resolves shadowing errors for estimating an accurate three-dimensional (3D) shape and underlying shadowless images. Experimental results on 3D surface reconstruction demonstrated the significance of the BDS method for simple shapes, such as an isolated line pattern. However, we found that complicated shapes, such as line-and-space (L&S) and multilayered patterns, produce deformed and inaccurate measurement results. This problem is due to brightness fluctuations in the SEM images, which are mainly caused by the energy fluctuations of the primary electron beam, variations in the electronic expanse inside a specimen, and electrical charging of specimens. Despite these being essential difficulties encountered in SEM photometric stereo, it is difficult to model accurately all the complicated physical phenomena of electronic behavior. We improved the robustness of the surface reconstruction in order to deal with these practical difficulties with complicated shapes. Here, design data are useful clues as to the pattern layout and layer information of integrated semiconductors. We used the design data as a guide of the measured shape and incorporated a geometrical constraint term to evaluate the difference between the measured and designed shapes into the objective function of the BDS method. Because the true shape does not necessarily correspond to the designed one, we use an iterative scheme to develop proper guide patterns and a 3D surface that provides both a less distorted and more accurate 3D shape after convergence. Extensive experiments on real image data demonstrate the robustness and effectiveness of our method.

Electron Trapping and Charge Transport by Large Amplitude Whistlers

NASA Technical Reports Server (NTRS)

Kellogg, P. J.; Cattell, C. A.; Goetz, K.; Monson, S. J.; Wilson, L. B., III

2010-01-01

Trapping of electrons by magnetospheric whistlers is investigated using data from the Waves experiment on Wind and the S/WAVES experiment on STEREO. Waveforms often show a characteristic distortion which is shown to be due to electrons trapped in the potential of the electrostatic part of oblique whistlers. The density of trapped electrons is significant, comparable to that of the unperturbed whistler. Transport of these trapped electrons to new regions can generate potentials of several kilovolts, Trapping and the associated potentials may play an important role in the acceleration of Earth's radiation belt electrons.

Analysis of Performance of Stereoscopic-Vision Software

NASA Technical Reports Server (NTRS)

Kim, Won; Ansar, Adnan; Steele, Robert; Steinke, Robert

2007-01-01

A team of JPL researchers has analyzed stereoscopic vision software and produced a document describing its performance. This software is of the type used in maneuvering exploratory robotic vehicles on Martian terrain. The software in question utilizes correlations between portions of the images recorded by two electronic cameras to compute stereoscopic disparities, which, in conjunction with camera models, are used in computing distances to terrain points to be included in constructing a three-dimensional model of the terrain. The analysis included effects of correlation- window size, a pyramidal image down-sampling scheme, vertical misalignment, focus, maximum disparity, stereo baseline, and range ripples. Contributions of sub-pixel interpolation, vertical misalignment, and foreshortening to stereo correlation error were examined theoretically and experimentally. It was found that camera-calibration inaccuracy contributes to both down-range and cross-range error but stereo correlation error affects only the down-range error. Experimental data for quantifying the stereo disparity error were obtained by use of reflective metrological targets taped to corners of bricks placed at known positions relative to the cameras. For the particular 1,024-by-768-pixel cameras of the system analyzed, the standard deviation of the down-range disparity error was found to be 0.32 pixel.

Sunward-propagating Solar Energetic Electrons inside Multiple Interplanetary Flux Ropes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gómez-Herrero, Raúl; Hidalgo, Miguel A.; Carcaboso, Fernando

2017-05-10

On 2013 December 2 and 3, the SEPT and STE instruments on board STEREO-A observed two solar energetic electron events with unusual sunward-directed fluxes. Both events occurred during a time interval showing typical signatures of interplanetary coronal mass ejections (ICMEs). The electron timing and anisotropies, combined with extreme-ultraviolet solar imaging and radio wave spectral observations, are used to confirm the solar origin and the injection times of the energetic electrons. The solar source of the ICME is investigated using remote-sensing observations and a three-dimensional reconstruction technique. In situ plasma and magnetic field data combined with energetic electron observations and amore » flux-rope model are used to determine the ICME magnetic topology and the interplanetary electron propagation path from the Sun to 1 au. Two consecutive flux ropes crossed the STEREO-A location and each electron event occurred inside a different flux rope. In both cases, the electrons traveled from the solar source to 1 au along the longest legs of the flux ropes still connected to the Sun. During the December 2 event, energetic electrons propagated along the magnetic field, while during the December 3 event they were propagating against the field. As found by previous studies, the energetic electron propagation times are consistent with a low number of field line rotations N < 5 of the flux rope between the Sun and 1 au. The flux rope model used in this work suggests an even lower number of rotations.« less

Demonstration of a viable quantitative theory for interplanetary type II radio bursts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmidt, J. M., E-mail: jschmidt@physics.usyd.edu.au; Cairns, Iver H.

Between 29 November and 1 December 2013 the two widely separated spacecraft STEREO A and B observed a long lasting, intermittent, type II radio burst for the extended frequency range ≈ 4 MHz to 30 kHz, including an intensification when the shock wave of the associated coronal mass ejection (CME) reached STEREO A. We demonstrate for the first time our ability to quantitatively and accurately simulate the fundamental (F) and harmonic (H) emission of type II bursts from the higher corona (near 11 solar radii) to 1 AU. Our modeling requires the combination of data-driven three-dimensional magnetohydrodynamic simulations for the CME andmore » plasma background, carried out with the BATS-R-US code, with an analytic quantitative kinetic model for both F and H radio emission, including the electron reflection at the shock, growth of Langmuir waves and radio waves, and the radiations propagation to an arbitrary observer. The intensities and frequencies of the observed radio emissions vary hugely by factors ≈ 10{sup 6} and ≈ 10{sup 3}, respectively; the theoretical predictions are impressively accurate, being typically in error by less than a factor of 10 and 20 %, for both STEREO A and B. We also obtain accurate predictions for the timing and characteristics of the shock and local radio onsets at STEREO A, the lack of such onsets at STEREO B, and the z-component of the magnetic field at STEREO A ahead of the shock, and in the sheath. Very strong support is provided by these multiple agreements for the theory, the efficacy of the BATS-R-US code, and the vision of using type IIs and associated data-theory iterations to predict whether a CME will impact Earth’s magnetosphere and drive space weather events.« less

Demonstration of a viable quantitative theory for interplanetary type II radio bursts

NASA Astrophysics Data System (ADS)

Schmidt, J. M.; Cairns, Iver H.

2016-03-01

Between 29 November and 1 December 2013 the two widely separated spacecraft STEREO A and B observed a long lasting, intermittent, type II radio burst for the extended frequency range ≈ 4 MHz to 30 kHz, including an intensification when the shock wave of the associated coronal mass ejection (CME) reached STEREO A. We demonstrate for the first time our ability to quantitatively and accurately simulate the fundamental (F) and harmonic (H) emission of type II bursts from the higher corona (near 11 solar radii) to 1 AU. Our modeling requires the combination of data-driven three-dimensional magnetohydrodynamic simulations for the CME and plasma background, carried out with the BATS-R-US code, with an analytic quantitative kinetic model for both F and H radio emission, including the electron reflection at the shock, growth of Langmuir waves and radio waves, and the radiations propagation to an arbitrary observer. The intensities and frequencies of the observed radio emissions vary hugely by factors ≈ 106 and ≈ 103, respectively; the theoretical predictions are impressively accurate, being typically in error by less than a factor of 10 and 20 %, for both STEREO A and B. We also obtain accurate predictions for the timing and characteristics of the shock and local radio onsets at STEREO A, the lack of such onsets at STEREO B, and the z-component of the magnetic field at STEREO A ahead of the shock, and in the sheath. Very strong support is provided by these multiple agreements for the theory, the efficacy of the BATS-R-US code, and the vision of using type IIs and associated data-theory iterations to predict whether a CME will impact Earth's magnetosphere and drive space weather events.

Depth Acuity Methodology for Electronic 3D Displays: eJames (eJ)

DTIC Science & Technology

2016-07-01

AFRL-RH-WP-TR-2016-0060 Depth Acuity Methodology for Electronic 3D Displays: eJames (eJ) Eric L. Heft, John McIntire...AND SUBTITLE Depth Acuity Methodology for Electronic 3D Displays: eJames (eJ) 5a. CONTRACT NUMBER FA8650-08-D-6801-0050 5b. GRANT NUMBER...of 3D electronic displays: one active-eyewear Stereo 3D (S3D) and two non-eyewear full parallax Field-of-Light Display (FoLD) systems. The two FoLD

High voltage electron microscopy studies of axoplasmic transport in neurons: a possible regulatory role for divalent cations

PubMed Central

1982-01-01

Light and high voltage electron microscopy (HVEM) procedures have been employed to examine the processes regulating saltatory motion in neurons. Light microscope studies demonstrate that organelle transport occurs by rapid bidirectional saltations along linear pathways in cultured neuroblastoma cells. HVEM stereo images of axons reveal that microtubules (Mts) and organelles are suspended in a continuous latticework of fine microtrabecular filaments and that the Mts and lattice constitute a basic cytoskeletal structure mediating the motion of particles along axons. We propose that particle transport depends on dynamic properties of nonstatic microtrabecular lattice components. EXperiments were initiated to determine the effects of changes in divalent cation concentrations (Ca2+ and Mg2+) on: (a)the continuation of transport and (b) the corresponding structural properties of the microtrabecular lattice. We discovered that transport continues or is stimulated to a limited extent in cells exposed to small amounts of exogenously supplied Ca2+ and Mg2+ ions (less than 0.1 mM). Exposure of neurons to increased dosages of Ca2+ and Mg2+ (0.2-1.0 mM) stimulates transport for 2-4 min at 37 degrees C, but after a 5- to 20-min exposure the saltatory movements of organelles are observed gradually to become shorter in duration and rate particle motion ceases to occur. HVEM observations demonstrated that Ca2+ - and with the cessation of motion. Ca2+-containing solutions produced contractions of the microtrabecular filaments, whereas Mg2+-containing solutions had the opposing effect of stimulating an elongation and assembly (expansion) of microtrabeculae. On the basis of these observations we hypothesize that cycles of Ca2+/Mg2+-coupled contractions and expansions of the microtrabecular lattice probably regulate organelle motion in nerve cells. PMID:6177704

Ultrastructural networks in growth cones and neurites of cultured central nervous system neurons.

PubMed Central

Tsui, H C; Ris, H; Klein, W L

1983-01-01

We have examined growth cones and neurites of cultured central nervous system neurons by high-voltage electron microscopy. Embryonic chicken retina cells were cultured on polylysine-treated and Formvar-coated gold grids for 2-6 days, fixed, and critical point dried. Growth cones and neurites were examined as unembedded whole mounts. Three-dimensional images from stereo-pair electron micrographs of these regions showed a high degree of ultrastructural articulation, with distinct, non-tapering filaments (5-9 nm in diameter) joining both cytoskeletal and membranous components. In the central regions of growth cones, interconnected structures included microtubules, large membranous sacs (up to 400 nm), and irregular vesicles (25-75 nm). A denser filamentous network was prevalent at the edges of growth cones. This network, which frequently adjoined the surface membrane, linked vesicles of uniform size (35-40 nm). Such vesicles often were seen densely packed in growth cone protrusions that were about the size of small synaptic boutons. Prevalent structural interconnections within growth cones conceivably could play a logistic role in specific membrane assembly, intracellular transport, endocytosis, and secretion. Because such processes are not unique to growth cones, the extensive linkages we have observed may have implications for cytoplasmic structure in general. Images PMID:6577454

Relationship between SEP Peak intensity and CME Acceleration, Speed and Width

NASA Astrophysics Data System (ADS)

Xie, H.; St Cyr, O. C.; Makela, P. A.; Gopalswamy, N.

2017-12-01

We study the large solar energetic particle (SEP) events that were detected by GOES in the >10 MeV energy channel during December 2006 to January 2016. Data used in this study includes the Solar Electron Proton Telescope (SEPT) and High Energy Telescopes (HET) on STEREO A and B, the Electron, Proton, and Alpha Monitor (EPAM) on ACE, and the Energetic and Relativistic Nuclei and Electron instrument (ERNE) on SOHO. By choosing the smallest connection angles between SEP solar locations and magnetic foot-points of each spacecraft, we divide SEP events as SOHO SEPs or STEREO SEPs. We then compute the SEP peak intensity I0 at the center of the Gausssian using the Gausssian expression from Richardson et al. (2014) and study the relationship between SEP electron and proton peak intensity and CME acceleration, speed and width. By using I0 derived from multi-spacecraft observations we found that the correlations between SEP peak intensity and CME acceleration and speed improved. We also found that this correlation can be further improved by taking into account the effects of CME width and its solar source latitude. The implication for the SEP forecast of our obtained results will be discussed.

STEREO and Wind Observations of Intense Cyclotron Harmonic Waves at the Earth's Bow Shock and Inside the Magnetosheath

NASA Technical Reports Server (NTRS)

Breneman, A. W.; Cattell, C.

2013-01-01

We present the first observations of electron cyclotron harmonic waves at the Earth's bow shock from STEREO and Wind burst waveform captures. These waves are observed at magnetic field gradients at a variety of shock geometries ranging from quasi-parallel to nearly perpendicular along with whistler mode waves, ion acoustic waves, and electrostatic solitary waves. Large amplitude cyclotron harmonic waveforms are also observed in the magnetosheath in association with magnetic field gradients convected past the bow shock. Amplitudes of the cyclotron harmonic waves range from a few tens to more than 500 millivolts/meter peak-peak. A comparison between the short (15 meters) and long (100 meters) Wind spin plane antennas shows a similar response at low harmonics and a stronger response on the short antenna at higher harmonics. This indicates that wavelengths are not significantly larger than 100 meters, consistent with the electron cyclotron radius. Waveforms are broadband and polarizations are distinctively comma-shaped with significant power both perpendicular and parallel to the magnetic field. Harmonics tend to be more prominent in the perpendicular directions. These observations indicate that the waves consist of a combination of perpendicular Bernstein waves and field-aligned waves without harmonics. A likely source is the electron cyclotron drift instability which is a coupling between Bernstein and ion acoustic waves. These waves are the most common type of high-frequency wave seen by STEREO during bow shock crossings and magnetosheath traversals and our observations suggest that they are an important component of the high-frequency turbulent spectrum in these regions.

The High Energy Telescopes for the STEREO Mission

NASA Astrophysics Data System (ADS)

von Rosenvinge, T.T.; Cummings, A.C.; Leske, R.A.; Mewaldt, R.A.; Reames, D.V.; Stone, E.C.; Wiedenbeck, M.E.

We describe the High Energy Telescopes (HETs), which are part of the IMPACT investigation for the STEREO mission (Principal Investigator: Janet Luhmann, University of California at Berkeley). The two STEREO spacecraft were launched from Cape Canaveral, FL on October 25, 2006. High energy electrons (~ 0.7 -6 MeV) and nuclei from hydrogen to iron (~ 13 - 200 MeV/nucleon) are detected by the HETs, one on each spacecraft. Observations from one pass through the Earth’s magnetosphere and from four X-class solar events in December, 2006 are presented to illustrate the capabilities of the HETs. The HET observations are also compared with observations from other spacecraft. The event of December 13th was the first Ground Level Event in almost two years. We will compare the elemental composition of this event with that of the previous Ground Level Event on January 20, 2005. This work was supported by NASA (at Caltech and JPL under contract NAS5-00133 and grant NAG5-12929).

Analysis of Cutmarks on Bone: Can Light Microscopy Be of Any Help?

PubMed

Cerutti, Elisa; Spagnoli, Laura; Araujo, Nadezhda; Gibelli, Daniele; Mazzarelli, Debora; Cattaneo, Cristina

2016-12-01

One of the main issues in forensic anthropology consists of the identification of signs of trauma in skeletal remains, including sharp-force injuries. So far, several studies have been performed to assess differences between injuries caused by different instruments, not, however, through light microscopy.In this study, 152 sharp-force injuries were performed by 5 different tools through 2 different orientations on 2 humeral diaphyses and were analyzed by stereo and light microscopy to assess possible morphological differences.This study showed that although W-shaped injuries are frequently reported in cases of wood-cutting saws, other shapes are often observed; lesions due to metal-cutting saws are almost always U shaped, whereas injuries caused by knives are V shaped. Although cut marks may represent a variable range of features, the present study was able to highlight typical profiles that may be of some help for the diagnosis of weapon and the intentionality of the action.

In Amnio MRI of Mouse Embryos

PubMed Central

Roberts, Thomas A.; Norris, Francesca C.; Carnaghan, Helen; Savery, Dawn; Wells, Jack A.; Siow, Bernard; Scambler, Peter J.; Pierro, Agostino; De Coppi, Paolo; Eaton, Simon; Lythgoe, Mark F.

2014-01-01

Mouse embryo imaging is conventionally carried out on ex vivo embryos excised from the amniotic sac, omitting vital structures and abnormalities external to the body. Here, we present an in amnio MR imaging methodology in which the mouse embryo is retained in the amniotic sac and demonstrate how important embryonic structures can be visualised in 3D with high spatial resolution (100 µm/px). To illustrate the utility of in amnio imaging, we subsequently apply the technique to examine abnormal mouse embryos with abdominal wall defects. Mouse embryos at E17.5 were imaged and compared, including three normal phenotype embryos, an abnormal embryo with a clear exomphalos defect, and one with a suspected gastroschisis phenotype. Embryos were excised from the mother ensuring the amnion remained intact and stereo microscopy was performed. Embryos were next embedded in agarose for 3D, high resolution MRI on a 9.4T scanner. Identification of the abnormal embryo phenotypes was not possible using stereo microscopy or conventional ex vivo MRI. Using in amnio MRI, we determined that the abnormal embryos had an exomphalos phenotype with varying severities. In amnio MRI is ideally suited to investigate the complex relationship between embryo and amnion, together with screening for other abnormalities located outside of the mouse embryo, providing a valuable complement to histology and existing imaging methods available to the phenotyping community. PMID:25330230

Use of stereo vision and 24-bit false-color imagery to enhance visualization of multimodal confocal images

NASA Astrophysics Data System (ADS)

Beltrame, Francesco; Diaspro, Alberto; Fato, Marco; Martin, I.; Ramoino, Paola; Sobel, Irwin E.

1995-03-01

Confocal microscopy systems can be linked to 3D data oriented devices for the interactive navigation of the operator through a 3D object space. Sometimes, such environments are named `virtual reality' or `augmented reality' systems. We consider optical confocal laser scanning microscopy images, in fluorescence with various excitations and emissions, and versus time The aim of our study has been the quantitative spatial analysis of confocal data using the false-color composition technique. Starting from three 2D confocal fluorescent images at the same slice location in a given biological specimen, a new single image representation of all three parameters has been generated by the false-color technique on a HP 9000/735 workstation, connected to the confocal microscope. The color composite result of the mapping of the three parameters is displayed using a resolution of 24 bits per pixel. The operator may independently vary the mix of each of the three components in the false-color composite via three (R, G, B) mixing sliders. Furthermore, by using the pixel data in the three fluorescent component images, a 3D space containing the density distribution of these three parameters has been constructed. The histogram has been displayed in stereo: it can be used for clustering purposes from the operator, through an original thresholding algorithm.

STEREO OBSERVATIONS OF FAST MAGNETOSONIC WAVES IN THE EXTENDED SOLAR CORONA ASSOCIATED WITH EIT/EUV WAVES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kwon, Ryun-Young; Ofman, Leon; Kramar, Maxim

2013-03-20

We report white-light observations of a fast magnetosonic wave associated with a coronal mass ejection observed by STEREO/SECCHI/COR1 inner coronagraphs on 2011 August 4. The wave front is observed in the form of density compression passing through various coronal regions such as quiet/active corona, coronal holes, and streamers. Together with measured electron densities determined with STEREO COR1 and Extreme UltraViolet Imager (EUVI) data, we use our kinematic measurements of the wave front to calculate coronal magnetic fields and find that the measured speeds are consistent with characteristic fast magnetosonic speeds in the corona. In addition, the wave front turns outmore » to be the upper coronal counterpart of the EIT wave observed by STEREO EUVI traveling against the solar coronal disk; moreover, stationary fronts of the EIT wave are found to be located at the footpoints of deflected streamers and boundaries of coronal holes, after the wave front in the upper solar corona passes through open magnetic field lines in the streamers. Our findings suggest that the observed EIT wave should be in fact a fast magnetosonic shock/wave traveling in the inhomogeneous solar corona, as part of the fast magnetosonic wave propagating in the extended solar corona.« less

A conjugated microporous polymer based visual sensing platform for aminoglycoside antibiotics in water.

PubMed

Bhunia, Subhajit; Dey, Nilanjan; Pradhan, Anirban; Bhattacharya, Santanu

2018-06-20

A donor-acceptor based conjugated microporous polymer, PER@NiP-CMOP-1, has been synthesized which can achieve highly sensitive stereo-specific "Turn ON" biosensing of an aminoglycoside up to the ppb level. The coordination-driven inhibition of photo-induced electron transfer (d-PET) for d-electrons and the rotational freezing are the key factors for the recovery of the emission.

SD-SEM: sparse-dense correspondence for 3D reconstruction of microscopic samples.

PubMed

Baghaie, Ahmadreza; Tafti, Ahmad P; Owen, Heather A; D'Souza, Roshan M; Yu, Zeyun

2017-06-01

Scanning electron microscopy (SEM) imaging has been a principal component of many studies in biomedical, mechanical, and materials sciences since its emergence. Despite the high resolution of captured images, they remain two-dimensional (2D). In this work, a novel framework using sparse-dense correspondence is introduced and investigated for 3D reconstruction of stereo SEM images. SEM micrographs from microscopic samples are captured by tilting the specimen stage by a known angle. The pair of SEM micrographs is then rectified using sparse scale invariant feature transform (SIFT) features/descriptors and a contrario RANSAC for matching outlier removal to ensure a gross horizontal displacement between corresponding points. This is followed by dense correspondence estimation using dense SIFT descriptors and employing a factor graph representation of the energy minimization functional and loopy belief propagation (LBP) as means of optimization. Given the pixel-by-pixel correspondence and the tilt angle of the specimen stage during the acquisition of micrographs, depth can be recovered. Extensive tests reveal the strength of the proposed method for high-quality reconstruction of microscopic samples. Copyright © 2017 Elsevier Ltd. All rights reserved.

Computer graphics of SEM images facilitate recognition of chromosome position in isolated human metaphase plates.

PubMed

Hodge, L D; Barrett, J M; Welter, D A

1995-04-01

There is general agreement that at the time of mitosis chromosomes occupy precise positions and that these positions likely affect subsequent nuclear function in interphase. However, before such ideas can be investigated in human cells, it is necessary to determine first the precise position of each chromosome with regard to its neighbors. It has occurred to us that stereo images, produced by scanning electron microscopy, of isolated metaphase plates could form the basis whereby these positions could be ascertained. In this paper we describe a computer graphic technique that permits us to keep track of individual chromosomes in a metaphase plate and to compare chromosome positions in different metaphase plates. Moreover, the computer graphics provide permanent, easily manipulated, rapid recall of stored chromosome profiles. These advantages are demonstrated by a comparison of the relative position of group A-specific and groups D- and G-specific chromosomes to the full complement of chromosomes in metaphase plates isolated from a nearly triploid human-derived cell (HeLa S3) to a hypo-diploid human fetal lung cell.

STEREO/SEPT particle observations during the CIR event on 2011 August 9

NASA Astrophysics Data System (ADS)

Dresing, N.; Heber, B.; Klassen, A.; Kühl, P.; Boettcher, S. I.; Gomez-Herrero, R.; Wraase, S.

2017-12-01

Among others, shocks are known to be accelerators of energetic charged particles. However, many questions regarding the acceleration efficiency and the required conditions are not fully understood. In particular, the acceleration of electrons by shocks is often questioned. Recurrent energetic particle events are caused by the passage of Corotating Interaction Regions (CIRs) that have been extensively analysed by different instrumentation close to Earth. Measurements of the Solar Electron and Proton Telescope aboard the Solar TErrestrial RElations Observatory are utilized in the solar heliospheric community to investigate electron events. Due to its measurement principle, the magnet foil technique, ions can contribute to the electron channel. This effect is well known. During recurrent energetic particle events the averaged helium to proton ration is enhanced to more than 10%. The energy per nucleon spectra are nearly the same for protons and helium. Although the electron intensity profile is influenced by an ion contamination during the shock crossings it is not obvious that electrons are not enhanced during such periods. Computation using a GEANT4 simulation of the SEPT instrument resulted in response function for ions and electrons. These response functions have been utilzed to analyze the recurrent energetic particle event that was was measured by STEREO B on August 9, 2011. Assuming a constant helium to proton ratio and energy spectra described by a Band function we found that electron and ion measurement can be explained by the contribution of helium and protons with an helium to proton ratio of about 16%. Thus no electron enhancements are needed to explain the SEPT measurements.

Passive perception system for day/night autonomous off-road navigation

NASA Astrophysics Data System (ADS)

Rankin, Arturo L.; Bergh, Charles F.; Goldberg, Steven B.; Bellutta, Paolo; Huertas, Andres; Matthies, Larry H.

2005-05-01

Passive perception of terrain features is a vital requirement for military related unmanned autonomous vehicle operations, especially under electromagnetic signature management conditions. As a member of Team Raptor, the Jet Propulsion Laboratory developed a self-contained passive perception system under the DARPA funded PerceptOR program. An environmentally protected forward-looking sensor head was designed and fabricated in-house to straddle an off-the-shelf pan-tilt unit. The sensor head contained three color cameras for multi-baseline daytime stereo ranging, a pair of cooled mid-wave infrared cameras for nighttime stereo ranging, and supporting electronics to synchronize captured imagery. Narrow-baseline stereo provided improved range data density in cluttered terrain, while wide-baseline stereo provided more accurate ranging for operation at higher speeds in relatively open areas. The passive perception system processed stereo images and outputted over a local area network terrain maps containing elevation, terrain type, and detected hazards. A novel software architecture was designed and implemented to distribute the data processing on a 533MHz quad 7410 PowerPC single board computer under the VxWorks real-time operating system. This architecture, which is general enough to operate on N processors, has been subsequently tested on Pentium-based processors under Windows and Linux, and a Sparc based-processor under Unix. The passive perception system was operated during FY04 PerceptOR program evaluations at Fort A. P. Hill, Virginia, and Yuma Proving Ground, Arizona. This paper discusses the Team Raptor passive perception system hardware and software design, implementation, and performance, and describes a road map to faster and improved passive perception.

Equipment and New Products

ERIC Educational Resources Information Center

Poitras, Adrian W., Ed.

1973-01-01

The following items are discussed: Digital Counters and Readout Devices, Automatic Burette Outfits, Noise Exposure System, Helium-Cadmium Laser, New pH Buffers and Flip-Top Dispenser, Voltage Calibrator Transfer Standard, Photomicrographic Stereo Zoom Microscope, Portable pH Meter, Micromanipulators, The Snuffer, Electronic Top-Loading Balances,…

LONGITUDINAL AND RADIAL DEPENDENCE OF SOLAR ENERGETIC PARTICLE PEAK INTENSITIES: STEREO, ACE, SOHO, GOES, AND MESSENGER OBSERVATIONS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lario, D.; Ho, G. C.; Decker, R. B.

Simultaneous measurements of solar energetic particle (SEP) events by two or more of the spacecraft located near 1 AU during the rising phase of solar cycle 24 (i.e., STEREO-A, STEREO-B, and near-Earth spacecraft such as ACE, SOHO, and GOES) are used to determine the longitudinal dependence of 71-112 keV electron, 0.7-3 MeV electron, 15-40 MeV proton, and 25-53 MeV proton peak intensities measured in the prompt component of SEP events. Distributions of the peak intensities for the selected 35 events with identifiable solar origin are approximated by the form exp [ - ({phi} - {phi}{sub 0}){sup 2}/2{sigma}{sup 2}], where {phi}more » is the longitudinal separation between the parent active region and the footpoint of the nominal interplanetary magnetic field (IMF) line connecting each spacecraft with the Sun, {phi}{sub 0} is the distribution centroid, and {sigma} determines the longitudinal gradient. The MESSENGER spacecraft, at helioradii R < 1 AU, allows us to determine a lower limit to the radial dependence of the 71-112 keV electron peak intensities measured along IMF lines. We find five events for which the nominal magnetic footpoint of MESSENGER was less than 20 Degree-Sign apart from the nominal footpoint of a spacecraft near 1 AU. Although the expected theoretical radial dependence for the peak intensity of the events observed along the same field line can be approximated by a functional form R {sup -{alpha}} with {alpha} < 3, we find two events for which {alpha} > 3. These two cases correspond to SEP events occurring in a complex interplanetary medium that favored the enhancement of peak intensities near Mercury but hindered the SEP transport to 1 AU.« less

KSC-06pd1235

NASA Image and Video Library

2006-06-26

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the Solar Electron and Proton Telescope (SEPT) is seen on the STEREO observatory "B." SEPT is part of the Solar Energetic Particles Package of four telescopes, all part of the In situ Measurements of Particles & CME Transients (IMPACT) instrument suite. STEREO, which stands for Solar Terrestrial Relations Observatory, consists of two spacecraft whose mission is to take measurements of the sun and solar wind in 3-D, for the first time. This new view will improve our understanding of space weather and its impact on the Earth. Preparations are under way for a liftoff aboard a Delta rocket no earlier than July 30. Photo credit: NASA/George Shelton

Treatment of subgingival implant surfaces with Teflon-coated sonic and ultrasonic scaler tips and various implant curettes. An in vitro study.

PubMed

Rühling, A; Kocher, T; Kreusch, J; Plagmann, H C

1994-03-01

Removal of plaque and calculus by means of sonic and ultrasonic scalers causes considerable damage to implants. With a view to avoiding the aggressive effects of these instruments, an experimental study was carried out for which conventional sonic and ultrasonic scalers were coated with Teflon. The effects of these instruments on implant surfaces was then compared with that of plastic and metal implant curettes. Stereo-microscopy, scanning electron microscopy and surface profilometry were used to detect and record damage to implant surfaces and changes in surface roughness. Generation and propagation of heat in subgingival simulation of use of sonic and ultrasonic scalers were also recorded by means of temperature measurements at the implant surface. The results revealed that no discernible damage was caused by Teflon-coated sonic and ultrasonic scalers or implant curettes made of plastic on smooth titanium surfaces. Instrument material residues were found on rough implant surfaces. It was not the intention of this study to provide an analysis of the prerequisites for the cleaning of rough implant surfaces, but rather to determine what type of damage is to be expected when contact is made with smooth and rough surfaces unintentionally. Temperature measurements during the subgingival use of sonic and ultrasonic scalers indicated satisfactory functioning of the cooling system. Coating of sonic and ultrasonic scaler tips with Teflon thus facilitates the use of high-frequency instruments to achieve professional cleaning of implants.

Observations of energetic particles between a pair of corotating interaction regions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, Z.; Chen, Y.; Tang, C. L.

We report observations of the acceleration and trapping of energetic ions and electrons between a pair of corotating interaction regions (CIRs). The event occurred in Carrington Rotation 2060. Observed by the STEREO-B spacecraft, the two CIRs were separated by less than 5 days. In contrast to other CIR events, the fluxes of the energetic ions and electrons in this event reached their maxima between the trailing edge of the first CIR and the leading edge of the second CIR. The radial magnetic field (B{sub r} ) reversed its sense and the anisotropy of the flux also changed from Sunward tomore » anti-Sunward between the two CIRs. Furthermore, there was an extended period of counterstreaming suprathermal electrons between the two CIRs. Similar observations for this event were also obtained with the Advanced Composition Explorer and STEREO-A. We conjecture that these observations were due to a U-shaped, large-scale magnetic field topology connecting the reverse shock of the first CIR and the forward shock of the second CIR. Such a disconnected U-shaped magnetic field topology may have formed due to magnetic reconnection in the upper corona.« less

How to Generate Chaos at Home.

ERIC Educational Resources Information Center

Smith, Douglas

1992-01-01

Describes an electronic circuit that can function as a prototype for chaotic systems. Specific applied voltages produce chaotic signals that can be viewed with an oscilloscope or be made audible with a home stereo system. Provides directions for assembly with typical costs, mathematical basis of chaos theory, and experimental extensions. (JJK)

New developments in electron microscopy for serial image acquisition of neuronal profiles.

PubMed

Kubota, Yoshiyuki

2015-02-01

Recent developments in electron microscopy largely automate the continuous acquisition of serial electron micrographs (EMGs), previously achieved by laborious manual serial ultrathin sectioning using an ultramicrotome and ultrastructural image capture process with transmission electron microscopy. The new systems cut thin sections and capture serial EMGs automatically, allowing for acquisition of large data sets in a reasonably short time. The new methods are focused ion beam/scanning electron microscopy, ultramicrotome/serial block-face scanning electron microscopy, automated tape-collection ultramicrotome/scanning electron microscopy and transmission electron microscope camera array. In this review, their positive and negative aspects are discussed. © The Author 2015. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Interpretation of increased energetic particle flux measurements by SEPT aboard the STEREO spacecraft and contamination

NASA Astrophysics Data System (ADS)

Wraase, S.; Heber, B.; Böttcher, S.; Bucik, R.; Dresing, N.; Gómez-Herrero, R.; Klassen, A.; Müller-Mellin, R.

2018-04-01

Context. Interplanetary (IP) shocks are known to be accelerators of energetic charged particles observed in-situ in the heliosphere. However, the acceleration of near-relativistic electrons by shocks in the interplanetary medium is often questioned. On 9 August 2011 a corotating interaction region (CIR) passed STEREO B (STB), which resulted in a flux increase in the electron and ion channels of the Solar Electron and Proton Telescope (SEPT). Because electron measurements in the few keV to several 100 keV range rely on the so-called magnet foil technique, which is utilized by SEPT, ions can contribute to the electron channels. Aim. We aim to investigate whether the flux increase in the electron channels of SEPT during the CIR event on 9 August 2011 is caused by ion contamination only. Methods: We compute the SEPT response functions for protons and helium utilizing an updated GEANT4 model of SEPT. The CIR energetic particle ion spectra for protons and helium are assumed to follow a Band function in energy per nucleon with a constant helium to proton ratio. Results: Our analysis leads to a helium to proton ratio of 16.9% and a proton flux following a Band function with the parameters I0 = 1.24 × 104 (cm2 s sr MeV nuc-1)-1, Ec = 79 keV nuc-1, and spectral indices of γ1 = -0.94 and γ2 = -3.80, which are in good agreement with measurements by the Suprathermal Ion Telescope (SIT) aboard STB. Conclusions: Since our results explain the SEPT measurements, we conclude that no significant amount of electrons were accelerated between 55 and 425 keV by the CIR.

Transition state analogue imprinted polymers as artificial amidases for amino acid p-nitroanilides: morphological effects of polymer network on catalytic efficiency.

PubMed

Mathew, Divya; Thomas, Benny; Devaky, K S

2017-11-13

The morphology of the polymer network - porous/less porous - plays predominant role in the amidase activities of the polymer catalysts in the hydrolytic reactions of amino acid p-nitroanilides. Polymers with the imprints of stable phosphonate analogue of the intermediate of hydrolytic reactions were synthesized as enzyme mimics. Molecular imprinting was carried out in thermodynamically stable porogen dimethyl sulphoxide and unstable porogen chloroform, to investigate the morphological effects of polymers on catalytic amidolysis. It was found that the medium of polymerization has vital influence in the amidase activities of the enzyme mimics. The morphological studies of the polymer catalysts were carried out by scanning electron microscopy and Bruner-Emmett-Teller analysis. The morphology of the polymer catalysts and their amidase activities are found to be dependent on the composition of reaction medium. The polymer catalyst prepared in dimethyl sulphoxide is observed to be efficient in 1:9 acetonitrile (ACN)-Tris HCl buffer and that prepared in chloroform is noticed to be stereo specifically and shape-selectively effective in 9:1 ACN-Tris HCl buffer. The solvent memory effect in catalytic amidolysis was investigated using the polymer prepared in acetonitrile.

The MVACS Robotic Arm Camera

NASA Astrophysics Data System (ADS)

Keller, H. U.; Hartwig, H.; Kramm, R.; Koschny, D.; Markiewicz, W. J.; Thomas, N.; Fernades, M.; Smith, P. H.; Reynolds, R.; Lemmon, M. T.; Weinberg, J.; Marcialis, R.; Tanner, R.; Boss, B. J.; Oquest, C.; Paige, D. A.

2001-08-01

The Robotic Arm Camera (RAC) is one of the key instruments newly developed for the Mars Volatiles and Climate Surveyor payload of the Mars Polar Lander. This lightweight instrument employs a front lens with variable focus range and takes images at distances from 11 mm (image scale 1:1) to infinity. Color images with a resolution of better than 50 μm can be obtained to characterize the Martian soil. Spectral information of nearby objects is retrieved through illumination with blue, green, and red lamp sets. The design and performance of the camera are described in relation to the science objectives and operation. The RAC uses the same CCD detector array as the Surface Stereo Imager and shares the readout electronics with this camera. The RAC is mounted at the wrist of the Robotic Arm and can characterize the contents of the scoop, the samples of soil fed to the Thermal Evolved Gas Analyzer, the Martian surface in the vicinity of the lander, and the interior of trenches dug out by the Robotic Arm. It can also be used to take panoramic images and to retrieve stereo information with an effective baseline surpassing that of the Surface Stereo Imager by about a factor of 3.

Whole-field macro- and micro-deformation characteristic of unbound water-loss in dentin hard tissue.

PubMed

Chen, Zhenning; Nadeau, Bobby; Yu, Kevin; Shao, Xinxing; He, Xiaoyuan; Goh, M Cynthia; Kishen, Anil

2018-04-06

High-resolution deformation measurements in a functionally graded hard tissue such as human dentin are essential to understand the unbound water-loss mediated changes and their role in its mechanical integrity. Yet a whole-field, 3-dimensional (3D) measurement and characterization of fully hydrated dentin in both macro- and micro-scales remain to be a challenge. This study was conducted in 2 stages. In stage-1, a stereo-digital image correlation approach was utilized to determine the water-loss and load-induced 3D deformations of teeth in a sagittal section over consecutively acquired frames, from a fully hydrated state to nonhydrated conditions for a period up to 2 hours. The macroscale analysis revealed concentrated residual deformations at the dentin-enamel-junction and the apical regions of root in the direction perpendicular to the dentinal tubules. Significant difference in the localized deformation characteristics was observed between the inner and outer aspects of the root dentin. During quasi-static loadings, further increase in the residual deformation was observed in the dentin. In stage-2, dentin microstructural variations induced by dynamic water-loss were assessed with environmental scanning electron microscopy and atomic force microscopy (AFM), showing that the dynamic water-loss induced distention of dentinal tubules with concave tubular edges, and concurrent contraction of intertubular dentin with convex profile. The findings from the current macro- and micro-scale analysis provided insight on the free-water-loss induced regional deformations and ultrastructural changes in human dentin. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Platinum replica electron microscopy: Imaging the cytoskeleton globally and locally.

PubMed

Svitkina, Tatyana M

2017-05-01

Structural studies reveal how smaller components of a system work together as a whole. However, combining high resolution of details with full coverage of the whole is challenging. In cell biology, light microscopy can image many cells in their entirety, but at a lower resolution, whereas electron microscopy affords very high resolution, but usually at the expense of the sample size and coverage. Structural analyses of the cytoskeleton are especially demanding, because cytoskeletal networks are unresolvable by light microscopy due to their density and intricacy, whereas their proper preservation is a challenge for electron microscopy. Platinum replica electron microscopy can uniquely bridge the gap between the "comfort zones" of light and electron microscopy by allowing high resolution imaging of the cytoskeleton throughout the entire cell and in many cells in the population. This review describes the principles and applications of platinum replica electron microscopy for studies of the cytoskeleton. Copyright © 2017 Elsevier Ltd. All rights reserved.

Platinum Replica Electron Microscopy: Imaging the Cytoskeleton Globally and Locally

PubMed Central

SVITKINA, Tatyana M.

2017-01-01

Structural studies reveal how smaller components of a system work together as a whole. However, combining high resolution of details with full coverage of the whole is challenging. In cell biology, light microscopy can image many cells in their entirety, but at a lower resolution, whereas electron microscopy affords very high resolution, but usually at the expense of the sample size and coverage. Structural analyses of the cytoskeleton are especially demanding, because cytoskeletal networks are unresolvable by light microscopy due to their density and intricacy, whereas their proper preservation is a challenge for electron microscopy. Platinum replica electron microscopy can uniquely bridge the gap between the “comfort zones” of light and electron microscopy by allowing high resolution imaging of the cytoskeleton throughout the entire cell and in many cells in the population. This review describes the principles and applications of platinum replica electron microscopy for studies of the cytoskeleton. PMID:28323208

The contribution of stereo vision to the control of braking.

PubMed

Tijtgat, Pieter; Mazyn, Liesbeth; De Laey, Christophe; Lenoir, Matthieu

2008-03-01

In this study the contribution of stereo vision to the control of braking in front of a stationary target vehicle was investigated. Participants with normal (StereoN) and weak (StereoW) stereo vision drove a go-cart along a linear track towards a stationary vehicle. They could start braking from a distance of 4, 7, or 10m from the vehicle. Deceleration patterns were measured by means of a laser. A lack of stereo vision was associated with an earlier onset of braking, but the duration of the braking manoeuvre was similar. During the deceleration, the time of peak deceleration occurred earlier in drivers with weak stereo vision. Stopping distance was greater in those lacking in stereo vision. A lack of stereo vision was associated with a more prudent brake behaviour, in which the driver took into account a larger safety margin. This compensation might be caused either by an unconscious adaptation of the human perceptuo-motor system, or by a systematic underestimation of distance remaining due to the lack of stereo vision. In general, a lack of stereo vision did not seem to increase the risk of rear-end collisions.

Silver stain for electron microscopy

NASA Technical Reports Server (NTRS)

Corbett, R. L.

1972-01-01

Ammoniacal silver stain used for light microscopy was adapted advantageously for use with very thin biological sections required for electron microscopy. Silver stain can be performed in short time, has more contrast, and is especially useful for low power electron microscopy.

Correlated Light and Electron Microscopy/Electron Tomography of Mitochondria In Situ

PubMed Central

Perkins, Guy A.; Sun, Mei G.; Frey, Terrence G.

2009-01-01

Three-dimensional light microscopy and three-dimensional electron microscopy (electron tomography) separately provide very powerful tools to study cellular structure and physiology, including the structure and physiology of mitochondria. Fluorescence microscopy allows one to study processes in live cells with specific labels and stains that follow the movement of labeled proteins and changes within cellular compartments but does not have sufficient resolution to define the ultrastructure of intracellular organelles such as mitochondria. Electron microscopy and electron tomography provide the highest resolution currently available to study mitochondrial ultrastructure but cannot follow processes in living cells. We describe the combination of these two techniques in which fluorescence confocal microscopy is used to study structural and physiologic changes in mitochondria within apoptotic HeLa cells to define the apoptotic timeframe. Cells can then be selected at various stages of the apoptotic timeframe for examination at higher resolution by electron microscopy and electron tomography. This is a form of “virtual” 4-dimensional electron microscopy that has revealed interesting structural changes in the mitochondria of HeLa cells during apoptosis. The same techniques can be applied, with modification, to study other dynamic processes within cells in other experimental contexts. PMID:19348881

Graphene-enabled electron microscopy and correlated super-resolution microscopy of wet cells.

PubMed

Wojcik, Michal; Hauser, Margaret; Li, Wan; Moon, Seonah; Xu, Ke

2015-06-11

The application of electron microscopy to hydrated biological samples has been limited by high-vacuum operating conditions. Traditional methods utilize harsh and laborious sample dehydration procedures, often leading to structural artefacts and creating difficulties for correlating results with high-resolution fluorescence microscopy. Here, we utilize graphene, a single-atom-thick carbon meshwork, as the thinnest possible impermeable and conductive membrane to protect animal cells from vacuum, thus enabling high-resolution electron microscopy of wet and untreated whole cells with exceptional ease. Our approach further allows for facile correlative super-resolution and electron microscopy of wet cells directly on the culturing substrate. In particular, individual cytoskeletal actin filaments are resolved in hydrated samples through electron microscopy and well correlated with super-resolution results.

Constraint-based stereo matching

NASA Technical Reports Server (NTRS)

Kuan, D. T.

1987-01-01

The major difficulty in stereo vision is the correspondence problem that requires matching features in two stereo images. Researchers describe a constraint-based stereo matching technique using local geometric constraints among edge segments to limit the search space and to resolve matching ambiguity. Edge segments are used as image features for stereo matching. Epipolar constraint and individual edge properties are used to determine possible initial matches between edge segments in a stereo image pair. Local edge geometric attributes such as continuity, junction structure, and edge neighborhood relations are used as constraints to guide the stereo matching process. The result is a locally consistent set of edge segment correspondences between stereo images. These locally consistent matches are used to generate higher-level hypotheses on extended edge segments and junctions to form more global contexts to achieve global consistency.

Stereo-hologram in discrete depth of field (Conference Presentation)

NASA Astrophysics Data System (ADS)

Lee, Kwanghoon; Park, Min-Chul

2017-05-01

In holographic space, continuous object space can be divided as several discrete spaces satisfied each of same depth of field (DoF). In the environment of wearable device using holography, specially, this concept can be applied to macroscopy filed in contrast of the field of microscopy. Since the former has not need to high depth resolution because perceiving power of eye in human visual system, it can distinguish clearly among the objects in depth space, has lower than optical power of microscopic field. Therefore continuous but discrete depth of field (DDoF) for whole object space can present the number of planes included sampled space considered its DoF. Each DoF plane has to consider the occlusion among the object's areas in its region to show the occluded phenomenon inducing by the visual axis around the eye field of view. It makes natural scene in recognition process even though the combined discontinuous DoF regions are altered to the continuous object space. Thus DDoF pull out the advantages such as saving consuming time of the calculation process making the hologram and the reconstruction. This approach deals mainly the properties of several factors required in stereo hologram HMD such as stereoscopic DoF according to the convergence, least number of DDoFs planes in normal visual circumstance (within to 10,000mm), the efficiency of saving time for taking whole holographic process under the our method compared to the existing. Consequently this approach would be applied directly to the stereo-hologram HMD field to embody a real-time holographic imaging.

Scanning ultrafast electron microscopy.

PubMed

Yang, Ding-Shyue; Mohammed, Omar F; Zewail, Ahmed H

2010-08-24

Progress has been made in the development of four-dimensional ultrafast electron microscopy, which enables space-time imaging of structural dynamics in the condensed phase. In ultrafast electron microscopy, the electrons are accelerated, typically to 200 keV, and the microscope operates in the transmission mode. Here, we report the development of scanning ultrafast electron microscopy using a field-emission-source configuration. Scanning of pulses is made in the single-electron mode, for which the pulse contains at most one or a few electrons, thus achieving imaging without the space-charge effect between electrons, and still in ten(s) of seconds. For imaging, the secondary electrons from surface structures are detected, as demonstrated here for material surfaces and biological specimens. By recording backscattered electrons, diffraction patterns from single crystals were also obtained. Scanning pulsed-electron microscopy with the acquired spatiotemporal resolutions, and its efficient heat-dissipation feature, is now poised to provide in situ 4D imaging and with environmental capability.

Microtubule dynamics in nerve cells: analysis using microinjection of biotinylated tubulin into PC12 cells

PubMed Central

1988-01-01

To study microtubule (MT) dynamics in nerve cells, we microinjected biotin-labeled tubulin into the cell body of chemically fused and differentiated PC12 cells and performed the immunofluorescence or immunogold procedure using an anti-biotin antibody followed by secondary antibodies coupled to fluorescent dye or colloidal gold. Incorporation of labeled subunits into the cytoskeleton of neurites was observed within minutes after microinjection. Serial electron microscopic reconstruction revealed that existing MTs in PC12 neurites incorporated labeled subunits mainly at their distal ends and the elongation rate of labeled segments was estimated to be less than 0.3 micron/min. Overall organization of MTs in the nerve cells was different from that in undifferentiated cells such as fibroblasts. Namely, we have not identified any MT-organizing centers from which labeled MTs are emanating in the cell bodies of the injected cells. Stereo electron microscopy revealed that some fully labeled segments seemed to start in the close vicinity of electron dense material within the neurites. This suggests new nucleation off some structures in the neurites. We have also studied the overall pattern of the incorporation of labeled subunits which extended progressively from the proximal part of the neurites toward their tips. To characterize the mechanism of tubulin incorporation, we have measured mean density of gold labeling per unit length of labeled segments at different parts of the neurites. The results indicate access of free tubulin subunits into the neurites and local incorporation into the neurite cytoskeleton. Our results lead to the conclusion that MTs are not static polymers but dynamic structures that continue to elongate even within the differentiated nerve cell processes. PMID:3047145

High-Resolution of Electron Microscopy of Montmorillonite and Montmorillonite/Epoxy Nanocomposites

DTIC Science & Technology

2005-01-01

AFRL-ML-WP-TP-2006-464 HIGH-RESOLUTION OF ELECTRON MICROSCOPY OF MONTMORILLONITE AND MONTMORILLONITE /EPOXY NANOCOMPOSITES Lawrence F...HIGH-RESOLUTION OF ELECTRON MICROSCOPY OF MONTMORILLONITE AND MONTMORILLONITE /EPOXY NANOCOMPOSITES 5c. PROGRAM ELEMENT NUMBER 62102F 5d...transmission electron microscopy the structure and morphology of montmorillonite (MMT), a material of current interest for use in polymer nanocomposites, was

Correlative fluorescence microscopy and scanning transmission electron microscopy of quantum-dot-labeled proteins in whole cells in liquid.

PubMed

Dukes, Madeline J; Peckys, Diana B; de Jonge, Niels

2010-07-27

Correlative fluorescence microscopy and transmission electron microscopy (TEM) is a state-of-the-art microscopy methodology to study cellular function, combining the functionality of light microscopy with the high resolution of electron microscopy. However, this technique involves complex sample preparation procedures due to its need for either thin sections or frozen samples for TEM imaging. Here, we introduce a novel correlative approach capable of imaging whole eukaryotic cells in liquid with fluorescence microscopy and with scanning transmission electron microscopy (STEM); there is no additional sample preparation necessary for the electron microscopy. Quantum dots (QDs) were bound to epidermal growth factor (EGF) receptors of COS7 fibroblast cells. Fixed whole cells in saline water were imaged with fluorescence microscopy and subsequently with STEM. The STEM images were correlated with fluorescence images of the same cellular regions. QDs of dimensions 7x12 nm were visible in a 5 microm thick layer of saline water, consistent with calculations. A spatial resolution of 3 nm was achieved on the QDs.

Correlative Fluorescence Microscopy and Scanning Transmission Electron Microscopy of Quantum Dot Labeled Proteins in Whole Cells in Liquid

PubMed Central

Dukes, Madeline J.; Peckys, Diana B.; de Jonge, Niels

2010-01-01

Correlative fluorescence microscopy and transmission electron microscopy (TEM) is a state-of-the-art microscopy methodology to study cellular function, combining the functionality of light microscopy with the high resolution of electron microscopy. However, this technique involves complex sample preparation procedures due to its need for either thin sections or frozen samples for TEM imaging. Here, we introduce a novel correlative approach capable of imaging whole eukaryotic cells in liquid with fluorescence microscopy and with scanning transmission electron microscopy (STEM); there is no additional sample preparation necessary for the electron microscopy. Quantum dots (QDs) were bound to epidermal growth factor (EGF) receptors of COS7 fibroblast cells. Fixed whole cells in saline water were imaged with fluorescence microscopy and subsequently with STEM. The STEM images were correlated with fluorescence images of the same cellular regions. QDs of dimensions 7 × 12 nm were visible in a 5 μm thick layer of saline water, consistent with calculations. A spatial resolution of 3 nm was achieved on the QDs. PMID:20550177

Strong non-radial propagation of energetic electrons in solar corona

NASA Astrophysics Data System (ADS)

Klassen, A.; Dresing, N.; Gómez-Herrero, R.; Heber, B.; Veronig, A.

2018-06-01

Analyzing the sequence of solar energetic electron events measured at both STEREO-A (STA) and STEREO-B (STB) spacecraft during 17-21 July 2014, when their orbital separation was 34°, we found evidence of a strong non-radial electron propagation in the solar corona below the solar wind source surface. The impulsive electron events were associated with recurrent flare and jet (hereafter flare/jet) activity at the border of an isolated coronal hole situated close to the solar equator. We have focused our study on the solar energetic particle (SEP) event on 17 July 2014, during which both spacecraft detected a similar impulsive and anisotropic energetic electron event suggesting optimal connection of both spacecraft to the parent particle source, despite the large angular separation between the parent flare and the nominal magnetic footpoints on the source surface of STA and STB of 68° and 90°, respectively. Combining the remote-sensing extreme ultraviolet (EUV) observations, in-situ plasma, magnetic field, and energetic particle data we investigated and discuss here the origin and the propagation trajectory of energetic electrons in the solar corona. We find that the energetic electrons in the energy range of 55-195 keV together with the associated EUV jet were injected from the flare site toward the spacecraft's magnetic footpoints and propagate along a strongly non-radial and inclined magnetic field below the source surface. From stereoscopic (EUV) observations we estimated the inclination angle of the jet trajectory and the respective magnetic field of 63° ± 11° relative to the radial direction. We show how the flare accelerated electrons reach very distant longitudes in the heliosphere, when the spacecraft are nominally not connected to the particle source. This example illustrates how ballistic backmapping can occasionally fail to characterize the magnetic connectivity during SEP events. This finding also provides an additional mechanism (one among others), which may explain the origin of widespread SEP events.

Electronic equipment vulnerability to fire released carbon fibers

NASA Technical Reports Server (NTRS)

Pride, R. A.; Mchatton, A. D.; Musselman, K. A.

1980-01-01

The vulnerability of electronic equipment to damage by carbon fibers released from burning aircraft type structural composite materials was investigated. Tests were conducted on commercially available stereo power amplifiers which showed that the equipment was damaged by fire released carbon fibers but not by the composite resin residue, soot and products of combustion of the fuel associated with burning the carbon fiber composites. Results indicate that the failure rates of the equipment exposed to the fire released fiber were consistent with predictions based on tests using virgin fibers.

Few-cycle carrier envelope phase-dependent stereo detection of electrons.

PubMed

Verhoef, Aart J; Fernández, Alma; Lezius, Matthias; O'Keeffe, Kevin; Uiberacker, Matthias; Krausz, Ferenc

2006-12-01

The spatial distribution of electrons emitted from atoms by few-cycle optical fields is known to be dependent on the carrier envelope phase, i.e., the phase of the field with respect to the pulse envelope. With respect to Paulus et al. [Phys. Rev. Lett.91, 253004 (2003)] we propose a greatly simplified device to measure and control the carrier envelope phase of few-cycle pulses with an accuracy of better than pi/10 based on this principle. We compared different schemes to control the carrier envelope phase of our pulses.

Generalized parallel-perspective stereo mosaics from airborne video.

PubMed

Zhu, Zhigang; Hanson, Allen R; Riseman, Edward M

2004-02-01

In this paper, we present a new method for automatically and efficiently generating stereoscopic mosaics by seamless registration of images collected by a video camera mounted on an airborne platform. Using a parallel-perspective representation, a pair of geometrically registered stereo mosaics can be precisely constructed under quite general motion. A novel parallel ray interpolation for stereo mosaicing (PRISM) approach is proposed to make stereo mosaics seamless in the presence of obvious motion parallax and for rather arbitrary scenes. Parallel-perspective stereo mosaics generated with the PRISM method have better depth resolution than perspective stereo due to the adaptive baseline geometry. Moreover, unlike previous results showing that parallel-perspective stereo has a constant depth error, we conclude that the depth estimation error of stereo mosaics is in fact a linear function of the absolute depths of a scene. Experimental results on long video sequences are given.

Electron Microscopy.

ERIC Educational Resources Information Center

Beer, Michael

1980-01-01

Reviews technical aspects of structure determination in biological electron microscopy (EM). Discusses low dose EM, low temperature microscopy, electron energy loss spectra, determination of mass or molecular weight, and EM of labeled systems. Cites 34 references. (CS)

Relationship Between the Expansion Speed and Radial Speed of CMEs Confirmed Using Quadrature Observations from SOHO and STEREO

NASA Technical Reports Server (NTRS)

Gopalswamy, Nat; Makela, Pertti; Yashiro, Seiji

2011-01-01

It is difficult to measure the true speed of Earth-directed CMEs from a coronagraph along the Sun-Earth line because of the occulting disk. However, the expansion speed (the speed with which the CME appears to spread in the sky plane) can be measured by such coronagraph. In order to convert the expansion speed to radial speed (which is important for space weather applications) one can use empirical relationship between the two that assumes an average width for all CMEs. If we have the width information from quadrature observations, we can confirm the relationship between expansion and radial speeds derived by Gopalswamy et al. (2009, CEAB, 33, 115,2009). The STEREO spacecraft were in quadrature with SOHO (STEREO-A ahead of Earth by 87 and STEREO-B 94 behind Earth) on 2011 February 15, when a fast Earth-directed CME occurred. The CME was observed as a halo by the Large-Angle and Spectrometric Coronagraph (LASCO) on board SOHO. The sky-plane speed was measured by SOHO/LASCO as the expansion speed, while the radial speed was measured by STEREO-A and STEREO-B. In addition, STEREO-A and STEREO-B images measured the width of the CME, which is unknown from Earth view. From the SOHO and STEREO measurements, we confirm the relationship between the expansion speed (Vexp ) and radial speed (Vrad ) derived previously from geometrical considerations (Gopalswamy et al. 2009): Vrad = 1/2 (1 + cot w) Vexp, where w is the half width of the CME. STEREO-B images of the CME, we found that CME had a full width of 75 degrees, so w = 37.5 degrees. This gives the relation as Vrad = 1.15 Vexp. From LASCO observations, we measured Vexp = 897 km/s, so we get the radial speed as 1033 km/s. Direct measurement of radial speed from STEREO gives 945 km/s (STEREO-A) and 1057 km/s (STEREO-B). These numbers are different only by 2.3% and 8.5% (for STEREO-A and STEREO-B, respectively) from the computed value.

STEREO observations of insitu waves in the vicinity of interplanetary shocks

NASA Astrophysics Data System (ADS)

Golla, T.; MacDowall, R. J.

2017-12-01

We present the high time resolution observations of the in situ waves obtained by the time domain sampler (TDS) of the WAVES experiment on the STEREO spacecraft in the vicinity of typical quasi-perpendicular super-critical interplanetary shocks. We show that often Langmuir waves occur as coherent one dimensional magnetic field aligned wave packets in the upstream regions and persist over large distances. The characteristics of these wave packets are consistent with those of Langmuir solitons formed as a result of oscillatting two stream instability (OTSI). Very intense high frequency waves which are completely different from Langmuir waves occur in the transition regions. These waves occur as very incoherent emissions and exhibit broad fundamental and second harmonic spectral peaks. We identify these waves as electron acoustic waves excited by the electron beams in the transition regions. We also show that very intense low frequency ion sound waves occur in the downstream regions. We discuss the implications of these observations on the theories of (1) strong Langmuir turbulence, (2) beam stabilization, (3) emission mechanisms of solar type II radio bursts, (4) wave-particle interactions responsible for collisionless dissipation, and (5) heating of the downstream plasmas.

Wide Swath Stereo Mapping from Gaofen-1 Wide-Field-View (WFV) Images Using Calibration

PubMed Central

Chen, Shoubin; Liu, Jingbin; Huang, Wenchao

2018-01-01

The development of Earth observation systems has changed the nature of survey and mapping products, as well as the methods for updating maps. Among optical satellite mapping methods, the multiline array stereo and agile stereo modes are the most common methods for acquiring stereo images. However, differences in temporal resolution and spatial coverage limit their application. In terms of this issue, our study takes advantage of the wide spatial coverage and high revisit frequencies of wide swath images and aims at verifying the feasibility of stereo mapping with the wide swath stereo mode and reaching a reliable stereo accuracy level using calibration. In contrast with classic stereo modes, the wide swath stereo mode is characterized by both a wide spatial coverage and high-temporal resolution and is capable of obtaining a wide range of stereo images over a short period. In this study, Gaofen-1 (GF-1) wide-field-view (WFV) images, with total imaging widths of 800 km, multispectral resolutions of 16 m and revisit periods of four days, are used for wide swath stereo mapping. To acquire a high-accuracy digital surface model (DSM), the nonlinear system distortion in the GF-1 WFV images is detected and compensated for in advance. The elevation accuracy of the wide swath stereo mode of the GF-1 WFV images can be improved from 103 m to 30 m for a DSM with proper calibration, meeting the demands for 1:250,000 scale mapping and rapid topographic map updates and showing improved efficacy for satellite imaging. PMID:29494540

Imaging of endodontic biofilms by combined microscopy (FISH/cLSM - SEM).

PubMed

Schaudinn, C; Carr, G; Gorur, A; Jaramillo, D; Costerton, J W; Webster, P

2009-08-01

Scanning electron microscopy is a useful imaging approach for the visualization of bacterial biofilms in their natural environments including their medical and dental habitats, because it allows for the exploration of large surfaces with excellent resolution of topographic features. Most biofilms in nature, however, are embedded in a thick layer of extracellular matrix that prevents a clear identification of individual bacteria by scanning electron microscopy. The use of confocal laser scanning microscopy on the other hand in combination with fluorescence in situ hybridization enables the visualization of matrix embedded bacteria in multi-layered biofilms. In our study, fluorescence in situ hybridization/confocal laser scanning microscopy and scanning electron microscopy were applied to visualize bacterial biofilm in endodontic root canals. The resulting fluorescence in situ hybridization /confocal laser scanning microscopy and scanning electron microscopy and pictures were subsequently combined into one single image to provide high-resolution information on the location of hidden bacteria. The combined use of scanning electron microscopy and fluorescence in situ hybridization / confocal laser scanning microscopy has the potential to overcome the limits of each single technique.

New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography.

PubMed

Schorb, Martin; Gaechter, Leander; Avinoam, Ori; Sieckmann, Frank; Clarke, Mairi; Bebeacua, Cecilia; Bykov, Yury S; Sonnen, Andreas F-P; Lihl, Reinhard; Briggs, John A G

2017-02-01

Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same sample. Rare dynamic events or specific objects can be identified, targeted and imaged by electron microscopy or tomography. To combine it with structural studies using cryo-electron microscopy or tomography, fluorescence microscopy must be performed while maintaining the specimen vitrified at liquid-nitrogen temperatures and in a dry environment during imaging and transfer. Here we present instrumentation, software and an experimental workflow that improves the ease of use, throughput and performance of correlated cryo-fluorescence and cryo-electron microscopy. The new cryo-stage incorporates a specially modified high-numerical aperture objective lens and provides a stable and clean imaging environment. It is combined with a transfer shuttle for contamination-free loading of the specimen. Optimized microscope control software allows automated acquisition of the entire specimen area by cryo-fluorescence microscopy. The software also facilitates direct transfer of the fluorescence image and associated coordinates to the cryo-electron microscope for subsequent fluorescence-guided automated imaging. Here we describe these technological developments and present a detailed workflow, which we applied for automated cryo-electron microscopy and tomography of various specimens. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Scanning ultrafast electron microscopy

PubMed Central

Yang, Ding-Shyue; Mohammed, Omar F.; Zewail, Ahmed H.

2010-01-01

Progress has been made in the development of four-dimensional ultrafast electron microscopy, which enables space-time imaging of structural dynamics in the condensed phase. In ultrafast electron microscopy, the electrons are accelerated, typically to 200 keV, and the microscope operates in the transmission mode. Here, we report the development of scanning ultrafast electron microscopy using a field-emission-source configuration. Scanning of pulses is made in the single-electron mode, for which the pulse contains at most one or a few electrons, thus achieving imaging without the space-charge effect between electrons, and still in ten(s) of seconds. For imaging, the secondary electrons from surface structures are detected, as demonstrated here for material surfaces and biological specimens. By recording backscattered electrons, diffraction patterns from single crystals were also obtained. Scanning pulsed-electron microscopy with the acquired spatiotemporal resolutions, and its efficient heat-dissipation feature, is now poised to provide in situ 4D imaging and with environmental capability. PMID:20696933

STEREO Education and Public Outreach Efforts

NASA Technical Reports Server (NTRS)

Kucera, Therese

2007-01-01

STEREO has had a big year this year with its launch and the start of data collection. STEREO has mostly focused on informal educational venues, most notably with STEREO 3D images made available to museums through the NASA Museum Alliance. Other activities have involved making STEREO imagery available though the AMNH network and Viewspace, continued partnership with the Christa McAuliffe Planetarium, data sonification projects, preservice teacher training, and learning activity development.

Accuracy aspects of stereo side-looking radar. [analysis of its visual perception and binocular vision

NASA Technical Reports Server (NTRS)

Leberl, F. W.

1979-01-01

The geometry of the radar stereo model and factors affecting visual radar stereo perception are reviewed. Limits to the vertical exaggeration factor of stereo radar are defined. Radar stereo model accuracies are analyzed with respect to coordinate errors caused by errors of radar sensor position and of range, and with respect to errors of coordinate differences, i.e., cross-track distances and height differences.

Probabilistic fusion of stereo with color and contrast for bilayer segmentation.

PubMed

Kolmogorov, Vladimir; Criminisi, Antonio; Blake, Andrew; Cross, Geoffrey; Rother, Carsten

2006-09-01

This paper describes models and algorithms for the real-time segmentation of foreground from background layers in stereo video sequences. Automatic separation of layers from color/contrast or from stereo alone is known to be error-prone. Here, color, contrast, and stereo matching information are fused to infer layers accurately and efficiently. The first algorithm, Layered Dynamic Programming (LDP), solves stereo in an extended six-state space that represents both foreground/background layers and occluded regions. The stereo-match likelihood is then fused with a contrast-sensitive color model that is learned on-the-fly and stereo disparities are obtained by dynamic programming. The second algorithm, Layered Graph Cut (LGC), does not directly solve stereo. Instead, the stereo match likelihood is marginalized over disparities to evaluate foreground and background hypotheses and then fused with a contrast-sensitive color model like the one used in LDP. Segmentation is solved efficiently by ternary graph cut. Both algorithms are evaluated with respect to ground truth data and found to have similar performance, substantially better than either stereo or color/ contrast alone. However, their characteristics with respect to computational efficiency are rather different. The algorithms are demonstrated in the application of background substitution and shown to give good quality composite video output.

Serial block face scanning electron microscopy--the future of cell ultrastructure imaging.

PubMed

Hughes, Louise; Hawes, Chris; Monteith, Sandy; Vaughan, Sue

2014-03-01

One of the major drawbacks in transmission electron microscopy has been the production of three-dimensional views of cells and tissues. Currently, there is no one suitable 3D microscopy technique that answers all questions and serial block face scanning electron microscopy (SEM) fills the gap between 3D imaging using high-end fluorescence microscopy and the high resolution offered by electron tomography. In this review, we discuss the potential of the serial block face SEM technique for studying the three-dimensional organisation of animal, plant and microbial cells.

Virtual-stereo fringe reflection technique for specular free-form surface testing

NASA Astrophysics Data System (ADS)

Ma, Suodong; Li, Bo

2016-11-01

Due to their excellent ability to improve the performance of optical systems, free-form optics have attracted extensive interest in many fields, e.g. optical design of astronomical telescopes, laser beam expanders, spectral imagers, etc. However, compared with traditional simple ones, testing for such kind of optics is usually more complex and difficult which has been being a big barrier for the manufacture and the application of these optics. Fortunately, owing to the rapid development of electronic devices and computer vision technology, fringe reflection technique (FRT) with advantages of simple system structure, high measurement accuracy and large dynamic range is becoming a powerful tool for specular free-form surface testing. In order to obtain absolute surface shape distributions of test objects, two or more cameras are often required in the conventional FRT which makes the system structure more complex and the measurement cost much higher. Furthermore, high precision synchronization between each camera is also a troublesome issue. To overcome the aforementioned drawback, a virtual-stereo FRT for specular free-form surface testing is put forward in this paper. It is able to achieve absolute profiles with the help of only one single biprism and a camera meanwhile avoiding the problems of stereo FRT based on binocular or multi-ocular cameras. Preliminary experimental results demonstrate the feasibility of the proposed technique.

Quantitative Evaluation of Stereo Visual Odometry for Autonomous Vessel Localisation in Inland Waterway Sensing Applications

PubMed Central

Kriechbaumer, Thomas; Blackburn, Kim; Breckon, Toby P.; Hamilton, Oliver; Rivas Casado, Monica

2015-01-01

Autonomous survey vessels can increase the efficiency and availability of wide-area river environment surveying as a tool for environment protection and conservation. A key challenge is the accurate localisation of the vessel, where bank-side vegetation or urban settlement preclude the conventional use of line-of-sight global navigation satellite systems (GNSS). In this paper, we evaluate unaided visual odometry, via an on-board stereo camera rig attached to the survey vessel, as a novel, low-cost localisation strategy. Feature-based and appearance-based visual odometry algorithms are implemented on a six degrees of freedom platform operating under guided motion, but stochastic variation in yaw, pitch and roll. Evaluation is based on a 663 m-long trajectory (>15,000 image frames) and statistical error analysis against ground truth position from a target tracking tachymeter integrating electronic distance and angular measurements. The position error of the feature-based technique (mean of ±0.067 m) is three times smaller than that of the appearance-based algorithm. From multi-variable statistical regression, we are able to attribute this error to the depth of tracked features from the camera in the scene and variations in platform yaw. Our findings inform effective strategies to enhance stereo visual localisation for the specific application of river monitoring. PMID:26694411

Applications of microscopy to genetic therapy of cystic fibrosis and other human diseases.

PubMed

Moninger, Thomas O; Nessler, Randy A; Moore, Kenneth C

2006-01-01

Gene therapy has become an extremely important and active field of biomedical research. Microscopy is an integral component of this effort. This chapter presents an overview of imaging techniques used in our facility in support of cystic fibrosis gene therapy research. Instrumentation used in these studies includes light and confocal microscopy, transmission electron microscopy, and scanning electron microscopy. Techniques outlined include negative staining, cryo-electron microscopy, three-dimentional reconstruction, enzyme cytochemistry, immunocytochemistry, and fluorescence imaging.

Application of environmental scanning electron microscopy to determine biological surface structure.

PubMed

Kirk, S E; Skepper, J N; Donald, A M

2009-02-01

The use of environmental scanning electron microscopy in biology is growing as more becomes understood about the advantages and limitations of the technique. These are discussed and we include new evidence about the effect of environmental scanning electron microscopy imaging on the viability of mammalian cells. We show that although specimen preparation for high-vacuum scanning electron microscopy introduces some artefacts, there are also challenges in the use of environmental scanning electron microscopy, particularly at higher resolutions. This suggests the two technologies are best used in combination. We have used human monocyte-derived macrophages as a test sample, imaging their complicated and delicate membrane ruffles and protrusions. We have also explored the possibility of using environmental scanning electron microscopy for dynamic experiments, finding that mammalian cells cannot be imaged and kept alive in the environmental scanning electron microscopy. The dehydration step in which the cell surface is exposed causes irreversible damage, probably via loss of membrane integrity during liquid removal in the specimen chamber. Therefore, mammalian cells should be imaged after fixation where possible to protect against damage as a result of chamber conditions.

CINEMA (Cubesat for Ion, Neutral, Electron, MAgnetic fields)

NASA Astrophysics Data System (ADS)

Lin, R. P.; Parks, G. K.; Halekas, J. S.; Larson, D. E.; Eastwood, J. P.; Wang, L.; Sample, J. G.; Horbury, T. S.; Roelof, E. C.; Lee, D.; Seon, J.; Hines, J.; Vo, H.; Tindall, C.; Ho, J.; Lee, J.; Kim, K.

2009-12-01

The NSF-funded CINEMA mission will provide cutting-edge magnetospheric science and critical space weather measurements, including high sensitivity mapping and high cadence movies of ring current, >4 keV Energetic Neutral Atom (ENA), as well as in situ measurements of suprathermal electrons (>~2 keV) and ions (>~ 4 keV) in the auroral and ring current precipitation regions, all with ~1 keV FWHM resolution and uniform response up to ~100 keV. A Suprathermal Electron, Ion, Neutral (STEIN) instrument adds an electrostatic deflection system to the STEREO STE (SupraThermal Electron) 4-pixel silicon semiconductor sensor to separate ions from electrons and from ENAs up to ~20 keV. In addition, inboard and outboard (on an extendable 1m boom) magnetoresistive sensor magnetometers will provide high cadence 3-axis magnetic field measurements. A new attitude control system (ACS) uses torque coils, a solar aspect sensor and the magnetometers to de-tumble the 3u CINEMA spacecraft, then spin it up to ~1 rpm with the spin axis perpendicular to the ecliptic, so STEIN can sweep across most of the sky every minute. Ideally, CINEMA will be placed into a high inclination low earth orbit that crosses the auroral zone and cusp. An S-band transmitter will be used to provide > ~8 kbps orbit-average data downlink to the ~11m diameter antenna of the Berkeley Ground Station. Two more identical CINEMA spacecraft will be built by Kyung Hee University (KHU) in Korea under their World Class University (WCU) program, to provide stereo ENA imaging and multi-point in situ measurements. Furthermore, CINEMA’s development of miniature particle and magnetic field sensors, and cubesat-size spinning spacecraft will be important for future nanosatellite space missions.

Overview of Solar Radio Bursts and their Sources

NASA Astrophysics Data System (ADS)

Golla, Thejappa; MacDowall, Robert J.

2018-06-01

Properties of radio bursts emitted by the Sun at frequencies below tens of MHz are reviewed. In this frequency range, the most prominent radio emissions are those of solar type II, complex type III and solar type IV radio bursts, excited probably by the energetic electron populations accelerated in completely different environments: (1) type II bursts are due to non-relativistic electrons accelerated by the CME driven interplanetary shocks, (2) complex type III bursts are due to near-relativistic electrons accelerated either by the solar flare reconnection process or by the SEP shocks, and (3) type IV bursts are due to relativistic electrons, trapped in the post-eruption arcades behind CMEs; these relativistic electrons probably are accelerated by the continued reconnection processes occurring beneath the CME. These radio bursts, which can serve as the natural plasma probes traversing the heliosphere by providing information about various crucial space plasma parameters, are also an ideal instrument for investigating acceleration mechanisms responsible for the high energy particles. The rich collection of valuable high quality radio and high time resolution in situ wave data from the WAVES experiments of the STEREO A, STEREO B and WIND spacecraft has provided an unique opportunity to study these different radio phenomena and understand the complex physics behind their excitation. We have developed Monte Carlo simulation techniques to estimate the propagation effects on the observed characteristics of these low frequency radio bursts. We will present some of the new results and describe how one can use these radio burst observations for space weather studies. We will also describe some of the non-linear plasma processes detected in the source regions of both solar type III and type II radio bursts. The analysis and simulation techniques used in these studies will be of immense use for future space based radio observations.

A search for Ganymede stereo images and 3D mapping opportunities

NASA Astrophysics Data System (ADS)

Zubarev, A.; Nadezhdina, I.; Brusnikin, E.; Giese, B.; Oberst, J.

2017-10-01

We used 126 Voyager-1 and -2 as well as 87 Galileo images of Ganymede and searched for stereo images suitable for digital 3D stereo analysis. Specifically, we consider image resolutions, stereo angles, as well as matching illumination conditions of respective stereo pairs. Lists of regions and local areas with stereo coverage are compiled. We present anaglyphs and we selected areas, not previously discussed, for which we constructed Digital Elevation Models and associated visualizations. The terrain characteristics in the models are in agreement with our previous notion of Ganymede morphology, represented by families of lineaments and craters of various sizes and degradation stages. The identified areas of stereo coverage may serve as important reference targets for the Ganymede Laser Altimeter (GALA) experiment on the future JUICE (Jupiter Icy Moons Explorer) mission.

Fully Hydrated Yeast Cells Imaged with Electron Microscopy

PubMed Central

Peckys, Diana B.; Mazur, Peter; Gould, Kathleen L.; de Jonge, Niels

2011-01-01

We demonstrate electron microscopy of fully hydrated eukaryotic cells with nanometer resolution. Living Schizosaccaromyces pombe cells were loaded in a microfluidic chamber and imaged in liquid with scanning transmission electron microscopy (STEM). The native intracellular (ultra)structures of wild-type cells and three different mutants were studied without prior labeling, fixation, or staining. The STEM images revealed various intracellular components that were identified on the basis of their shape, size, location, and mass density. The maximal achieved spatial resolution in this initial study was 32 ± 8 nm, an order of magnitude better than achievable with light microscopy on pristine cells. Light-microscopy images of the same samples were correlated with the corresponding electron-microscopy images. Achieving synergy between the capabilities of light and electron microscopy, we anticipate that liquid STEM will be broadly applied to explore the ultrastructure of live cells. PMID:21575587

Fully hydrated yeast cells imaged with electron microscopy.

PubMed

Peckys, Diana B; Mazur, Peter; Gould, Kathleen L; de Jonge, Niels

2011-05-18

We demonstrate electron microscopy of fully hydrated eukaryotic cells with nanometer resolution. Living Schizosaccharomyces pombe cells were loaded in a microfluidic chamber and imaged in liquid with scanning transmission electron microscopy (STEM). The native intracellular (ultra)structures of wild-type cells and three different mutants were studied without prior labeling, fixation, or staining. The STEM images revealed various intracellular components that were identified on the basis of their shape, size, location, and mass density. The maximal achieved spatial resolution in this initial study was 32 ± 8 nm, an order of magnitude better than achievable with light microscopy on pristine cells. Light-microscopy images of the same samples were correlated with the corresponding electron-microscopy images. Achieving synergy between the capabilities of light and electron microscopy, we anticipate that liquid STEM will be broadly applied to explore the ultrastructure of live cells. Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Solar Eclipse Video Captured by STEREO-B

NASA Technical Reports Server (NTRS)

2007-01-01

No human has ever witnessed a solar eclipse quite like the one captured on this video. The NASA STEREO-B spacecraft, managed by the Goddard Space Center, was about a million miles from Earth , February 25, 2007, when it photographed the Moon passing in front of the sun. The resulting movie looks like it came from an alien solar system. The fantastically-colored star is our own sun as STEREO sees it in four wavelengths of extreme ultraviolet light. The black disk is the Moon. When we observe a lunar transit from Earth, the Moon appears to be the same size as the sun, a coincidence that produces intoxicatingly beautiful solar eclipses. The silhouette STEREO-B saw, on the other hand, was only a fraction of the Sun. The Moon seems small because of the STEREO-B location. The spacecraft circles the sun in an Earth-like orbit, but it lags behind Earth by one million miles. This means STEREO-B is 4.4 times further from the Moon than we are, and so the Moon looks 4.4 times smaller. This version of the STEREO-B eclipse movie is a composite of data from the coronagraph and extreme ultraviolet imager of the spacecraft. STEREO-B has a sister ship named STEREO-A. Both are on a mission to study the sun. While STEREO-B lags behind Earth, STEREO-A orbits one million miles ahead ('B' for behind, 'A' for ahead). The gap is deliberate as it allows the two spacecraft to capture offset views of the sun. Researchers can then combine the images to produce 3D stereo movies of solar storms. The two spacecraft were launched in Oct. 2006 and reached their stations on either side of Earth in January 2007.

STEREO Space Weather and the Space Weather Beacon

NASA Technical Reports Server (NTRS)

Biesecker, D. A.; Webb, D F.; SaintCyr, O. C.

2007-01-01

The Solar Terrestrial Relations Observatory (STEREO) is first and foremost a solar and interplanetary research mission, with one of the natural applications being in the area of space weather. The obvious potential for space weather applications is so great that NOAA has worked to incorporate the real-time data into their forecast center as much as possible. A subset of the STEREO data will be continuously downlinked in a real-time broadcast mode, called the Space Weather Beacon. Within the research community there has been considerable interest in conducting space weather related research with STEREO. Some of this research is geared towards making an immediate impact while other work is still very much in the research domain. There are many areas where STEREO might contribute and we cannot predict where all the successes will come. Here we discuss how STEREO will contribute to space weather and many of the specific research projects proposed to address STEREO space weather issues. We also discuss some specific uses of the STEREO data in the NOAA Space Environment Center.

Preliminary measurements of kinetic dust temperature using stereoscopic particle image velocimetry

NASA Astrophysics Data System (ADS)

Williams, Jeremiah; Thomas, Edward

2004-11-01

A dusty (or complex) plasma is a four-component system composed of ions, electrons, neutral particles and charged microparticles. The presence of the microparticle (i.e., dust) component alters the plasma environment, giving rise to a wide variety of new plasma phenomena. Recently, the Auburn Plasma Sciences Laboratory (PSL) has acquired and installed a stereoscopic PIV (stereo-PIV) diagnostic tool for dusty plasma investigations [Thomas, et. al., Phys. Plasmas, 11, L37 (2004)]. This presentation discusses the use of the stereo-PIV technique for determining the velocity space distribution function of the microparticle component of a dc glow discharge dusty plasma. These distribution functions are then used to make preliminary estimates of the kinetic temperature of the dust component. The data is compared to a simple energy balance model that relates the dust temperature to the electric field and neutral pressure.

A Heuristic Approach to Remove the Background Intensity on White-light Solar Images. I. STEREO /HI-1 Heliospheric Images

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stenborg, Guillermo; Howard, Russell A.

White-light coronal and heliospheric imagers observe scattering of photospheric light from both dust particles (the F-Corona) and free electrons in the corona (the K-corona). The separation of the two coronae is thus vitally important to reveal the faint K-coronal structures (e.g., streamers, co-rotating interaction regions, coronal mass ejections, etc.). However, the separation of the two coronae is very difficult, so we are content in defining a background corona that contains the F- and as little K- as possible. For both the LASCO-C2 and LASCO-C3 coronagraphs aboard the Solar and Heliospheric Observatory ( SOHO ) and the white-light imagers of themore » SECCHI suite aboard the Solar Terrestrial Relationships Observatory ( STEREO ), a time-dependent model of the background corona is generated from about a month of similar images. The creation of such models is possible because the missions carrying these instruments are orbiting the Sun at about 1 au. However, the orbit profiles for the upcoming Solar Orbiter and Solar Probe Plus missions are very different. These missions will have elliptic orbits with a rapidly changing radial distance, hence invalidating the techniques in use for the SOHO /LASCO and STEREO /SECCHI instruments. We have been investigating techniques to generate background models out of just single images that could be used for the Solar Orbiter Heliospheric Imager and the Wide-field Imager for the Solar Probe Plus packages on board the respective spacecraft. In this paper, we introduce a state-of-the-art, heuristic technique to create the background intensity models of STEREO /HI-1 data based solely on individual images, report on new results derived from its application, and discuss its relevance to instrumental and operational issues.« less

Solar Terrestrial Relations Observatory (STEREO)

NASA Technical Reports Server (NTRS)

Davila, Joseph M.; SaintCyr, O. C.

2003-01-01

The solar magnetic field is constantly generated beneath the surface of the Sun by the solar dynamo. To balance this flux generation, there is constant dissipation of magnetic flux at and above the solar surface. The largest phenomenon associated with this dissipation is the Coronal Mass Ejection (CME). The Solar and Heliospheric Observatory (SOHO) has provided remarkable views of the corona and CMEs, and served to highlight how these large interplanetary disturbances can have terrestrial consequences. STEREO is the next logical step to study the physics of CME origin, propagation, and terrestrial effects. Two spacecraft with identical instrument complements will be launched on a single launch vehicle in November 2007. One spacecraft will drift ahead and the second behind the Earth at a separation rate of 22 degrees per year. Observation from these two vantage points will for the first time allow the observation of the three-dimensional structure of CMEs and the coronal structures where they originate. Each STEREO spacecraft carries a complement of 10 instruments, which include (for the first time) an extensive set of both remote sensing and in-situ instruments. The remote sensing suite is capable of imaging CMEs from the solar surface out to beyond Earth's orbit (1 AU), and in-situ instruments are able to measure distribution functions for electrons, protons, and ions over a broad energy range, from the normal thermal solar wind plasma to the most energetic solar particles. It is anticipated that these studies will ultimately lead to an increased understanding of the CME process and provide unique observations of the flow of energy from the corona to the near-Earth environment. An international research program, the International Heliophysical Year (IHY) will provide a framework for interpreting STEREO data in the context of global processes in the Sun-Earth system.

Sampling artifacts in perspective and stereo displays

NASA Astrophysics Data System (ADS)

Pfautz, Jonathan D.

2001-06-01

The addition of stereo cues to perspective displays is generally expected to improve the perception of depth. However, the display's pixel array samples both perspective and stereo depth cues, introducing inaccuracies and inconsistencies into the representation of an object's depth. The position, size and disparity of an object will be inaccurately presented and size and disparity will be inconsistently presented across depth. These inconsistencies can cause the left and right edges of an object to appear at different stereo depths. This paper describes how these inconsistencies result in conflicts between stereo and perspective depth information. A relative depth judgement task was used to explore these conflicts. Subjects viewed two objects and reported which appeared closer. Three conflicts resulting from inconsistencies caused by sampling were examined: (1) Perspective size and location versus stereo disparity. (2) Perspective size versus perspective location and stereo disparity. (3) Left and right edge disparity versus perspective size and location. In the first two cases, subjects achieved near-perfect accuracy when perspective and disparity cues were complementary. When size and disparity were inconsistent and thus in conflict, stereo dominated perspective. Inconsistency between the disparities of the horizontal edges of an object confused the subjects, even when complementary perspective and stereo information was provided. Since stereo was the dominant cue and was ambiguous across the object, this led to significantly reduced accuracy. Edge inconsistencies also led to more complaints about visual fatigue and discomfort.

Demonstration of transmission high energy electron microscopy

DOE PAGES

Merrill, F. E.; Goett, J.; Gibbs, J. W.; ...

2018-04-06

High energy electrons have been used to investigate an extension of transmission electron microscopy. This technique, transmission high energy electron microscopy (THEEM), provides two additional capabilities to electron microscopy. First, high energy electrons are more penetrating than low energy electrons, and thus, they are able to image through thicker samples. Second, the accelerating mode of a radio-frequency linear accelerator provides fast exposures, down to 1 ps, which are ideal for flash radiography, making THEEM well suited to study the evolution of fast material processes under dynamic conditions. Lastly, initial investigations with static objects and during material processing have been performedmore » to investigate the capabilities of this technique.« less

An Evaluation of the Effectiveness of Stereo Slides in Teaching Geomorphology.

ERIC Educational Resources Information Center

Giardino, John R.; Thornhill, Ashton G.

1984-01-01

Provides information about producing stereo slides and their use in the classroom. Describes an evaluation of the teaching effectiveness of stereo slides using two groups of 30 randomly selected students from introductory geomorphology. Results from a pretest/postttest measure show that stereo slides significantly improved understanding. (JM)

Introduction: A Symposium in Honor of Professor Sir John Meurig Thomas

NASA Astrophysics Data System (ADS)

Gai, P. L.; Saka, H.; Tomokiyo, Y.; Boyes, E. D.

2002-02-01

This issue is dedicated to Professor Sir John Meurig Thomas for his renowned contributions to electron microscopy in the chemical sciences. It is a collection of peer-reviewed leading articles in electron microscopy, based on the presentations at the Microscopy and Microanalysis (M&M) 2000 symposium, which was held to honor Professor Thomas's exceptional scientific leadership and wide-ranging fundamental contributions in the chemical applications of electron microscopy.The issue contains key papers by leading international researchers on the recent developments and applications of electron microscopy in the solid state and liquid state sciences. They include synthesis and characterization of silicon nitride nanorods, nanostructures of amorphous silica, electron microscopy studies of nanoscale structure and chemistry of Pt-Ru electrocatalysts of interest in direct methanol fuel cells, development of in situ wet-environmental transmission electron microscopy for the first nanoscale studies of dynamic liquid-catalyst reactions, strain analysis of silicon by finite element method and energy filtering convergent beam electron diffraction, applications of chemistry with electron microscopy, bismuth nanowires for applications in nanoelectronics technology, synthesis and characterization of quantum dots for superlattices and in situ electron microscopy at very high temperatures to study the motion of W5Si3 on [alpha][beta]-SiN3 substrates.We thank all the participants, including the invited speakers, contributors, and session chairs, who made the symposium successful. We also thank the authors and reviewers of the papers who worked assiduously towards the publication of this issue.We are very grateful to the Microscopy Society of America (MSA) for providing the opportunity to honor Professor Sir John Meurig Thomas. Organizational support from the MSA is also gratefully acknowledged.We thank Charles E. Lyman, editor in chief of Microscopy and Microanalysis for coordinating the publication of this issue and the entire journal staff for their efforts.

A correlative optical microscopy and scanning electron microscopy approach to locating nanoparticles in brain tumors.

PubMed

Kempen, Paul J; Kircher, Moritz F; de la Zerda, Adam; Zavaleta, Cristina L; Jokerst, Jesse V; Mellinghoff, Ingo K; Gambhir, Sanjiv S; Sinclair, Robert

2015-01-01

The growing use of nanoparticles in biomedical applications, including cancer diagnosis and treatment, demands the capability to exactly locate them within complex biological systems. In this work a correlative optical and scanning electron microscopy technique was developed to locate and observe multi-modal gold core nanoparticle accumulation in brain tumor models. Entire brain sections from mice containing orthotopic brain tumors injected intravenously with nanoparticles were imaged using both optical microscopy to identify the brain tumor, and scanning electron microscopy to identify the individual nanoparticles. Gold-based nanoparticles were readily identified in the scanning electron microscope using backscattered electron imaging as bright spots against a darker background. This information was then correlated to determine the exact location of the nanoparticles within the brain tissue. The nanoparticles were located only in areas that contained tumor cells, and not in the surrounding healthy brain tissue. This correlative technique provides a powerful method to relate the macro- and micro-scale features visible in light microscopy with the nanoscale features resolvable in scanning electron microscopy. Copyright © 2014 Elsevier Ltd. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Buck, E.C.; Dietz, N.L.; Bates, J.K.

Uranium contaminated soils from the Fernald Operation Site, Ohio, have been examined by a combination of optical microscopy, scanning electron microscopy with backscattered electron detection (SEM/BSE), and analytical electron microscopy (AEM). A method is described for preparing of transmission electron microscopy (TEM) thin sections by ultramicrotomy. By using these thin sections, SEM and TEM images can be compared directly. Uranium was found in iron oxides, silicates (soddyite), phosphates (autunites), and fluorite. Little uranium was associated with clays. The distribution of uranium phases was found to be inhomogeneous at the microscopic level.

Electron Microscopy of Ebola Virus-Infected Cells.

PubMed

Noda, Takeshi

2017-01-01

Ebola virus (EBOV) replicates in host cells, where both viral and cellular components show morphological changes during the process of viral replication from entry to budding. These steps in the replication cycle can be studied using electron microscopy (EM), including transmission electron microscopy (TEM) and scanning electron microscopy (SEM), which is one of the most useful methods for visualizing EBOV particles and EBOV-infected cells at the ultrastructural level. This chapter describes conventional methods for EM sample preparation of cultured cells infected with EBOV.

Optimization of Stereo Matching in 3D Reconstruction Based on Binocular Vision

NASA Astrophysics Data System (ADS)

Gai, Qiyang

2018-01-01

Stereo matching is one of the key steps of 3D reconstruction based on binocular vision. In order to improve the convergence speed and accuracy in 3D reconstruction based on binocular vision, this paper adopts the combination method of polar constraint and ant colony algorithm. By using the line constraint to reduce the search range, an ant colony algorithm is used to optimize the stereo matching feature search function in the proposed search range. Through the establishment of the stereo matching optimization process analysis model of ant colony algorithm, the global optimization solution of stereo matching in 3D reconstruction based on binocular vision system is realized. The simulation results show that by the combining the advantage of polar constraint and ant colony algorithm, the stereo matching range of 3D reconstruction based on binocular vision is simplified, and the convergence speed and accuracy of this stereo matching process are improved.

In Brief: NASA's Phoenix spacecraft lands on Mars

NASA Astrophysics Data System (ADS)

Showstack, Randy; Kumar, Mohi

2008-06-01

After a 9.5-month, 679-million-kilometer flight from Florida, NASA's Phoenix spacecraft made a soft landing in Vastitas Borealis in Mars's northern polar region on 25 May. The lander, whose camera already has returned some spectacular images, is on a 3-month mission to examine the area and dig into the soil of this site-chosen for its likelihood of having frozen water near the surface-and analyze samples. In addition to a robotic arm and robotic arm camera, the lander's instruments include a surface stereo imager; thermal and evolved-gas analyzer; microscopy, electrochemistry, and conductivity analyzer; and a meteorological station that is tracking daily weather and seasonal changes.

A direct electron detector for time-resolved MeV electron microscopy

DOE PAGES

Vecchione, T.; Denes, P.; Jobe, R. K.; ...

2017-03-15

The introduction of direct electron detectors enabled the structural biology revolution of cryogenic electron microscopy. Direct electron detectors are now expected to have a similarly dramatic impact on time-resolved MeV electron microscopy, particularly by enabling both spatial and temporal jitter correction. Here in this paper, we report on the commissioning of a direct electron detector for time-resolved MeV electron microscopy. The direct electron detector demonstrated MeV single electron sensitivity and is capable of recording megapixel images at 180 Hz. The detector has a 15-bit dynamic range, better than 30-μm spatial resolution and less than 20 analogue-to-digital converter count RMS pixelmore » noise. The unique capabilities of the direct electron detector and the data analysis required to take advantage of these capabilities are presented. The technical challenges associated with generating and processing large amounts of data are also discussed.« less

A direct electron detector for time-resolved MeV electron microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vecchione, T.; Denes, P.; Jobe, R. K.

The introduction of direct electron detectors enabled the structural biology revolution of cryogenic electron microscopy. Direct electron detectors are now expected to have a similarly dramatic impact on time-resolved MeV electron microscopy, particularly by enabling both spatial and temporal jitter correction. Here we report on the commissioning of a direct electron detector for time-resolved MeV electron microscopy. The direct electron detector demonstrated MeV single electron sensitivity and is capable of recording megapixel images at 180 Hz. The detector has a 15-bit dynamic range, better than 30-μmμm spatial resolution and less than 20 analogue-to-digital converter count RMS pixel noise. The uniquemore » capabilities of the direct electron detector and the data analysis required to take advantage of these capabilities are presented. The technical challenges associated with generating and processing large amounts of data are also discussed.« less

A direct electron detector for time-resolved MeV electron microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vecchione, T.; Denes, P.; Jobe, R. K.

The introduction of direct electron detectors enabled the structural biology revolution of cryogenic electron microscopy. Direct electron detectors are now expected to have a similarly dramatic impact on time-resolved MeV electron microscopy, particularly by enabling both spatial and temporal jitter correction. Here in this paper, we report on the commissioning of a direct electron detector for time-resolved MeV electron microscopy. The direct electron detector demonstrated MeV single electron sensitivity and is capable of recording megapixel images at 180 Hz. The detector has a 15-bit dynamic range, better than 30-μm spatial resolution and less than 20 analogue-to-digital converter count RMS pixelmore » noise. The unique capabilities of the direct electron detector and the data analysis required to take advantage of these capabilities are presented. The technical challenges associated with generating and processing large amounts of data are also discussed.« less

The Relationship Between the Expansion Speed and Radial Speed of CMEs Confirmed Using Quadrature Observations of the 2011 February 15 CME

NASA Astrophysics Data System (ADS)

Gopalswamy, N.; Makela, P.; Yashiro, S.; Davila, J. M.

2012-08-01

It is difficult to measure the true speed of Earth-directed CMEs from a coronagraph along the Sun-Earth line because of the occulting disk. However, the expansion speed (the speed with which the CME appears to spread in the sky plane) can be measured by such coronagraph. In order to convert the expansion speed to radial speed (which is important for space weather applications) one can use empirical relationship between the two that assumes an average width for all CMEs. If we have the width information from quadrature observations, we can confirm the relationship between expansion and radial speeds derived by Gopalswamy et al. (2009a). The STEREO spacecraft were in qudrature with SOHO (STEREO-A ahead of Earth by 87oand STEREO-B 94obehind Earth) on 2011 February 15, when a fast Earth-directed CME occurred. The CME was observed as a halo by the Large-Angle and Spectrometric Coronagraph (LASCO) on board SOHO. The sky-plane speed was measured by SOHO/LASCO as the expansion speed, while the radial speed was measured by STEREO-A and STEREO-B. In addition, STEREO-A and STEREO-B images measured the width of the CME, which is unknown from Earth view. From the SOHO and STEREO measurements, we confirm the relationship between the expansion speed (Vexp) and radial speed (Vrad) derived previously from geometrical considerations (Gopalswamy et al. 2009a): Vrad=1/2 (1 + cot w)Vexp, where w is the half width of the CME. STEREO-B images of the CME, we found that CME had a full width of 7 6o, so w=3 8o. This gives the relation as Vrad=1.1 4 Vexp. From LASCO observations, we measured Vexp=897 km/s, so we get the radial speed as 10 2 3 km/s. Direct measurement of radial speed yields 945 km/s (STEREO-A) and 105 8 km/s (STEREO-B). These numbers are different only by 7.6 % and 3.4 % (for STEREO-A and STEREO-B, respectively) from the computed value.

Visualization of electronic density

DOE PAGES

Grosso, Bastien; Cooper, Valentino R.; Pine, Polina; ...

2015-04-22

An atom’s volume depends on its electronic density. Although this density can only be evaluated exactly for hydrogen-like atoms, there are many excellent numerical algorithms and packages to calculate it for other materials. 3D visualization of charge density is challenging, especially when several molecular/atomic levels are intertwined in space. We explore several approaches to 3D charge density visualization, including the extension of an anaglyphic stereo visualization application based on the AViz package to larger structures such as nanotubes. We will describe motivations and potential applications of these tools for answering interesting questions about nanotube properties.

Correlative Stochastic Optical Reconstruction Microscopy and Electron Microscopy

PubMed Central

Kim, Doory; Deerinck, Thomas J.; Sigal, Yaron M.; Babcock, Hazen P.; Ellisman, Mark H.; Zhuang, Xiaowei

2015-01-01

Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure. Recent development of super-resolution fluorescence microscopy allows the location of molecules to be determined with nanometer-scale spatial resolution. However, correlative super-resolution fluorescence microscopy and electron microscopy (EM) still remains challenging because the optimal specimen preparation and imaging conditions for super-resolution fluorescence microscopy and EM are often not compatible. Here, we have developed several experiment protocols for correlative stochastic optical reconstruction microscopy (STORM) and EM methods, both for un-embedded samples by applying EM-specific sample preparations after STORM imaging and for embedded and sectioned samples by optimizing the fluorescence under EM fixation, staining and embedding conditions. We demonstrated these methods using a variety of cellular targets. PMID:25874453

High-resolution imaging by scanning electron microscopy of semithin sections in correlation with light microscopy.

PubMed

Koga, Daisuke; Kusumi, Satoshi; Shodo, Ryusuke; Dan, Yukari; Ushiki, Tatsuo

2015-12-01

In this study, we introduce scanning electron microscopy (SEM) of semithin resin sections. In this technique, semithin sections were adhered on glass slides, stained with both uranyl acetate and lead citrate, and observed with a backscattered electron detector at a low accelerating voltage. As the specimens are stained in the same manner as conventional transmission electron microscopy (TEM), the contrast of SEM images of semithin sections was similar to TEM images of ultrathin sections. Using this technique, wide areas of semithin sections were also observed by SEM, without the obstruction of grids, which was inevitable for traditional TEM. This study also applied semithin section SEM to correlative light and electron microscopy. Correlative immunofluorescence microscopy and immune-SEM were performed in semithin sections of LR white resin-embedded specimens using a FluoroNanogold-labeled secondary antibody. Because LR white resin is hydrophilic and electron stable, this resin is suitable for immunostaining and SEM observation. Using correlative microscopy, the precise localization of the primary antibody was demonstrated by fluorescence microscopy and SEM. This method has great potential for studies examining the precise localization of molecules, including Golgi- and ER-associated proteins, in correlation with LM and SEM. © The Author 2015. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

On the Progress of Scanning Transmission Electron Microscopy (STEM) Imaging in a Scanning Electron Microscope.

PubMed

Sun, Cheng; Müller, Erich; Meffert, Matthias; Gerthsen, Dagmar

2018-04-01

Transmission electron microscopy (TEM) with low-energy electrons has been recognized as an important addition to the family of electron microscopies as it may avoid knock-on damage and increase the contrast of weakly scattering objects. Scanning electron microscopes (SEMs) are well suited for low-energy electron microscopy with maximum electron energies of 30 keV, but they are mainly used for topography imaging of bulk samples. Implementation of a scanning transmission electron microscopy (STEM) detector and a charge-coupled-device camera for the acquisition of on-axis transmission electron diffraction (TED) patterns, in combination with recent resolution improvements, make SEMs highly interesting for structure analysis of some electron-transparent specimens which are traditionally investigated by TEM. A new aspect is correlative SEM, STEM, and TED imaging from the same specimen region in a SEM which leads to a wealth of information. Simultaneous image acquisition gives information on surface topography, inner structure including crystal defects and qualitative material contrast. Lattice-fringe resolution is obtained in bright-field STEM imaging. The benefits of correlative SEM/STEM/TED imaging in a SEM are exemplified by structure analyses from representative sample classes such as nanoparticulates and bulk materials.

Wisdom Appliance Control System

NASA Astrophysics Data System (ADS)

Hendrick; Jheng, Jyun-Teng; Tsai, Chen-Chai; Liou, Jia-Wei; Wang, Zhi-Hao; Jong, Gwo-Jia

2017-07-01

Intelligent appliances wisdom involves security, home care, convenient and energy saving, but the home automation system is still one of the core unit, and also using micro-processing electronics technology to centralized and control the home electrical products and systems, such as: lighting, television, fan, air conditioning, stereo, it composed of front-controller systems and back-controller panels, user using front-controller to control command, and then through the back-controller to powered the device.

Solar Eclipse, STEREO Style

NASA Technical Reports Server (NTRS)

2007-01-01

There was a transit of the Moon across the face of the Sun - but it could not be seen from Earth. This sight was visible only from the STEREO-B spacecraft in its orbit about the sun, trailing behind the Earth. NASA's STEREO mission consists of two spacecraft launched in October, 2006 to study solar storms. The transit starts at 1:56 am EST and continued for 12 hours until 1:57 pm EST. STEREO-B is currently about 1 million miles from the Earth, 4.4 times farther away from the Moon than we are on Earth. As the result, the Moon will appear 4.4 times smaller than what we are used to. This is still, however, much larger than, say, the planet Venus appeared when it transited the Sun as seen from Earth in 2004. This alignment of STEREO-B and the Moon is not just due to luck. It was arranged with a small tweak to STEREO-B's orbit last December. The transit is quite useful to STEREO scientists for measuring the focus and the amount of scattered light in the STEREO imagers and for determining the pointing of the STEREO coronagraphs. The Sun as it appears in these the images and each frame of the movie is a composite of nearly simultaneous images in four different wavelengths of extreme ultraviolet light that were separated into color channels and then recombined with some level of transparency for each.

Electron transparent graphene windows for environmental scanning electron microscopy in liquids and dense gases.

PubMed

Stoll, Joshua D; Kolmakov, Andrei

2012-12-21

Due to its ultrahigh electron transmissivity in a wide electron energy range, molecular impermeability, high electrical conductivity and excellent mechanical stiffness, suspended graphene membranes appear to be a nearly ideal window material for in situ (in vivo) environmental electron microscopy of nano- and mesoscopic objects (including bio-medical samples) immersed in liquids and/or in dense gaseous media. In this paper, taking advantage of a small modification of the graphene transfer protocol onto metallic and SiN supporting orifices, reusable environmental cells with exchangeable graphene windows have been designed. Using colloidal gold nanoparticles (50 nm) dispersed in water as model objects for scanning electron microscopy in liquids as proof of concept, different conditions for imaging through the graphene membrane were tested. Limiting factors for electron microscopy in liquids, such as electron beam induced water radiolysis and damage of the graphene membrane at high electron doses, are discussed.

Ultrafast Science Opportunities with Electron Microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Durr, Hermann

X-rays and electrons are two of the most fundamental probes of matter. When the Linac Coherent Light Source (LCLS), the world’s first x-ray free electron laser, began operation in 2009, it transformed ultrafast science with the ability to generate laser-like x-ray pulses from the manipulation of relativistic electron beams. This document describes a similar future transformation. In Transmission Electron Microscopy, ultrafast relativistic (MeV energy) electron pulses can achieve unsurpassed spatial and temporal resolution. Ultrafast temporal resolution will be the next frontier in electron microscopy and can ideally complement ultrafast x-ray science done with free electron lasers. This document describes themore » Grand Challenge science opportunities in chemistry, material science, physics and biology that arise from an MeV ultrafast electron diffraction & microscopy facility, especially when coupled with linac-based intense THz and X-ray pump capabilities.« less

Acquisition of stereo panoramas for display in VR environments

NASA Astrophysics Data System (ADS)

Ainsworth, Richard A.; Sandin, Daniel J.; Schulze, Jurgen P.; Prudhomme, Andrew; DeFanti, Thomas A.; Srinivasan, Madhusudhanan

2011-03-01

Virtual reality systems are an excellent environment for stereo panorama displays. The acquisition and display methods described here combine high-resolution photography with surround vision and full stereo view in an immersive environment. This combination provides photographic stereo-panoramas for a variety of VR displays, including the StarCAVE, NexCAVE, and CORNEA. The zero parallax point used in conventional panorama photography is also the center of horizontal and vertical rotation when creating photographs for stereo panoramas. The two photographically created images are displayed on a cylinder or a sphere. The radius from the viewer to the image is set at approximately 20 feet, or at the object of major interest. A full stereo view is presented in all directions. The interocular distance, as seen from the viewer's perspective, displaces the two spherical images horizontally. This presents correct stereo separation in whatever direction the viewer is looking, even up and down. Objects at infinity will move with the viewer, contributing to an immersive experience. Stereo panoramas created with this acquisition and display technique can be applied without modification to a large array of VR devices having different screen arrangements and different VR libraries.

JAVA Stereo Display Toolkit

NASA Technical Reports Server (NTRS)

Edmonds, Karina

2008-01-01

This toolkit provides a common interface for displaying graphical user interface (GUI) components in stereo using either specialized stereo display hardware (e.g., liquid crystal shutter or polarized glasses) or anaglyph display (red/blue glasses) on standard workstation displays. An application using this toolkit will work without modification in either environment, allowing stereo software to reach a wider audience without sacrificing high-quality display on dedicated hardware. The toolkit is written in Java for use with the Swing GUI Toolkit and has cross-platform compatibility. It hooks into the graphics system, allowing any standard Swing component to be displayed in stereo. It uses the OpenGL graphics library to control the stereo hardware and to perform the rendering. It also supports anaglyph and special stereo hardware using the same API (application-program interface), and has the ability to simulate color stereo in anaglyph mode by combining the red band of the left image with the green/blue bands of the right image. This is a low-level toolkit that accomplishes simply the display of components (including the JadeDisplay image display component). It does not include higher-level functions such as disparity adjustment, 3D cursor, or overlays all of which can be built using this toolkit.

Development of a Stereo Vision Measurement System for a 3D Three-Axial Pneumatic Parallel Mechanism Robot Arm

PubMed Central

Chiang, Mao-Hsiung; Lin, Hao-Ting; Hou, Chien-Lun

2011-01-01

In this paper, a stereo vision 3D position measurement system for a three-axial pneumatic parallel mechanism robot arm is presented. The stereo vision 3D position measurement system aims to measure the 3D trajectories of the end-effector of the robot arm. To track the end-effector of the robot arm, the circle detection algorithm is used to detect the desired target and the SAD algorithm is used to track the moving target and to search the corresponding target location along the conjugate epipolar line in the stereo pair. After camera calibration, both intrinsic and extrinsic parameters of the stereo rig can be obtained, so images can be rectified according to the camera parameters. Thus, through the epipolar rectification, the stereo matching process is reduced to a horizontal search along the conjugate epipolar line. Finally, 3D trajectories of the end-effector are computed by stereo triangulation. The experimental results show that the stereo vision 3D position measurement system proposed in this paper can successfully track and measure the fifth-order polynomial trajectory and sinusoidal trajectory of the end-effector of the three- axial pneumatic parallel mechanism robot arm. PMID:22319408

Stereo and IMU-Assisted Visual Odometry for Small Robots

NASA Technical Reports Server (NTRS)

2012-01-01

This software performs two functions: (1) taking stereo image pairs as input, it computes stereo disparity maps from them by cross-correlation to achieve 3D (three-dimensional) perception; (2) taking a sequence of stereo image pairs as input, it tracks features in the image sequence to estimate the motion of the cameras between successive image pairs. A real-time stereo vision system with IMU (inertial measurement unit)-assisted visual odometry was implemented on a single 750 MHz/520 MHz OMAP3530 SoC (system on chip) from TI (Texas Instruments). Frame rates of 46 fps (frames per second) were achieved at QVGA (Quarter Video Graphics Array i.e. 320 240), or 8 fps at VGA (Video Graphics Array 640 480) resolutions, while simultaneously tracking up to 200 features, taking full advantage of the OMAP3530's integer DSP (digital signal processor) and floating point ARM processors. This is a substantial advancement over previous work as the stereo implementation produces 146 Mde/s (millions of disparities evaluated per second) in 2.5W, yielding a stereo energy efficiency of 58.8 Mde/J, which is 3.75 better than prior DSP stereo while providing more functionality.

Microstructure of milk

USDA-ARS?s Scientific Manuscript database

The fat and protein in milk may be examined by scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy, and any bacteria present may be viewed by light microscopy. The fat exists as globules, the bulk of the protein is in the form of casein micelles, a...

Improved stereo matching applied to digitization of greenhouse plants

NASA Astrophysics Data System (ADS)

Zhang, Peng; Xu, Lihong; Li, Dawei; Gu, Xiaomeng

2015-03-01

The digitization of greenhouse plants is an important aspect of digital agriculture. Its ultimate aim is to reconstruct a visible and interoperable virtual plant model on the computer by using state-of-the-art image process and computer graphics technologies. The most prominent difficulties of the digitization of greenhouse plants include how to acquire the three-dimensional shape data of greenhouse plants and how to carry out its realistic stereo reconstruction. Concerning these issues an effective method for the digitization of greenhouse plants is proposed by using a binocular stereo vision system in this paper. Stereo vision is a technique aiming at inferring depth information from two or more cameras; it consists of four parts: calibration of the cameras, stereo rectification, search of stereo correspondence and triangulation. Through the final triangulation procedure, the 3D point cloud of the plant can be achieved. The proposed stereo vision system can facilitate further segmentation of plant organs such as stems and leaves; moreover, it can provide reliable digital samples for the visualization of greenhouse tomato plants.

Real-Time Visualization Tool Integrating STEREO, ACE, SOHO and the SDO

NASA Astrophysics Data System (ADS)

Schroeder, P. C.; Luhmann, J. G.; Marchant, W.

2011-12-01

The STEREO/IMPACT team has developed a new web-based visualization tool for near real-time data from the STEREO instruments, ACE and SOHO as well as relevant models of solar activity. This site integrates images, solar energetic particle, solar wind plasma and magnetic field measurements in an intuitive way using near real-time products from NOAA and other sources to give an overview of recent space weather events. This site enhances the browse tools already available at UC Berkeley, UCLA and Caltech which allow users to visualize similar data from the start of the STEREO mission. Our new near real-time tool utilizes publicly available real-time data products from a number of missions and instruments, including SOHO LASCO C2 images from the SOHO team's NASA site, SDO AIA images from the SDO team's NASA site, STEREO IMPACT SEP data plots and ACE EPAM data plots from the NOAA Space Weather Prediction Center and STEREO spacecraft positions from the STEREO Science Center.

Stereoscopy and the Human Visual System

PubMed Central

Banks, Martin S.; Read, Jenny C. A.; Allison, Robert S.; Watt, Simon J.

2012-01-01

Stereoscopic displays have become important for many applications, including operation of remote devices, medical imaging, surgery, scientific visualization, and computer-assisted design. But the most significant and exciting development is the incorporation of stereo technology into entertainment: specifically, cinema, television, and video games. In these applications for stereo, three-dimensional (3D) imagery should create a faithful impression of the 3D structure of the scene being portrayed. In addition, the viewer should be comfortable and not leave the experience with eye fatigue or a headache. Finally, the presentation of the stereo images should not create temporal artifacts like flicker or motion judder. This paper reviews current research on stereo human vision and how it informs us about how best to create and present stereo 3D imagery. The paper is divided into four parts: (1) getting the geometry right, (2) depth cue interactions in stereo 3D media, (3) focusing and fixating on stereo images, and (4) how temporal presentation protocols affect flicker, motion artifacts, and depth distortion. PMID:23144596

Stereo Cameras for Clouds (STEREOCAM) Instrument Handbook

DOE Office of Scientific and Technical Information (OSTI.GOV)

Romps, David; Oktem, Rusen

2017-10-31

The three pairs of stereo camera setups aim to provide synchronized and stereo calibrated time series of images that can be used for 3D cloud mask reconstruction. Each camera pair is positioned at approximately 120 degrees from the other pair, with a 17o-19o pitch angle from the ground, and at 5-6 km distance from the U.S. Department of Energy (DOE) Central Facility at the Atmospheric Radiation Measurement (ARM) Climate Research Facility Southern Great Plains (SGP) observatory to cover the region from northeast, northwest, and southern views. Images from both cameras of the same stereo setup can be paired together tomore » obtain 3D reconstruction by triangulation. 3D reconstructions from the ring of three stereo pairs can be combined together to generate a 3D mask from surrounding views. This handbook delivers all stereo reconstruction parameters of the cameras necessary to make 3D reconstructions from the stereo camera images.« less

Development of a stereo 3-D pictorial primary flight display

NASA Technical Reports Server (NTRS)

Nataupsky, Mark; Turner, Timothy L.; Lane, Harold; Crittenden, Lucille

1989-01-01

Computer-generated displays are becoming increasingly popular in aerospace applications. The use of stereo 3-D technology provides an opportunity to present depth perceptions which otherwise might be lacking. In addition, the third dimension could also be used as an additional dimension along which information can be encoded. Historically, the stereo 3-D displays have been used in entertainment, in experimental facilities, and in the handling of hazardous waste. In the last example, the source of the stereo images generally has been remotely controlled television camera pairs. The development of a stereo 3-D pictorial primary flight display used in a flight simulation environment is described. The applicability of stereo 3-D displays for aerospace crew stations to meet the anticipated needs for 2000 to 2020 time frame is investigated. Although, the actual equipment that could be used in an aerospace vehicle is not currently available, the lab research is necessary to determine where stereo 3-D enhances the display of information and how the displays should be formatted.

Fabrication of [001]-oriented tungsten tips for high resolution scanning tunneling microscopy

PubMed Central

Chaika, A. N.; Orlova, N. N.; Semenov, V. N.; Postnova, E. Yu.; Krasnikov, S. A.; Lazarev, M. G.; Chekmazov, S. V.; Aristov, V. Yu.; Glebovsky, V. G.; Bozhko, S. I.; Shvets, I. V.

2014-01-01

The structure of the [001]-oriented single crystalline tungsten probes sharpened in ultra-high vacuum using electron beam heating and ion sputtering has been studied using scanning and transmission electron microscopy. The electron microscopy data prove reproducible fabrication of the single-apex tips with nanoscale pyramids grained by the {011} planes at the apexes. These sharp, [001]-oriented tungsten tips have been successfully utilized in high resolution scanning tunneling microscopy imaging of HOPG(0001), SiC(001) and graphene/SiC(001) surfaces. The electron microscopy characterization performed before and after the high resolution STM experiments provides direct correlation between the tip structure and picoscale spatial resolution achieved in the experiments. PMID:24434734

Towards native-state imaging in biological context in the electron microscope

PubMed Central

Weston, Anne E.; Armer, Hannah E. J.

2009-01-01

Modern cell biology is reliant on light and fluorescence microscopy for analysis of cells, tissues and protein localisation. However, these powerful techniques are ultimately limited in resolution by the wavelength of light. Electron microscopes offer much greater resolution due to the shorter effective wavelength of electrons, allowing direct imaging of sub-cellular architecture. The harsh environment of the electron microscope chamber and the properties of the electron beam have led to complex chemical and mechanical preparation techniques, which distance biological samples from their native state and complicate data interpretation. Here we describe recent advances in sample preparation and instrumentation, which push the boundaries of high-resolution imaging. Cryopreparation, cryoelectron microscopy and environmental scanning electron microscopy strive to image samples in near native state. Advances in correlative microscopy and markers enable high-resolution localisation of proteins. Innovation in microscope design has pushed the boundaries of resolution to atomic scale, whilst automatic acquisition of high-resolution electron microscopy data through large volumes is finally able to place ultrastructure in biological context. PMID:19916039

An overview of state-of-the-art image restoration in electron microscopy.

PubMed

Roels, J; Aelterman, J; Luong, H Q; Lippens, S; Pižurica, A; Saeys, Y; Philips, W

2018-06-08

In Life Science research, electron microscopy (EM) is an essential tool for morphological analysis at the subcellular level as it allows for visualization at nanometer resolution. However, electron micrographs contain image degradations such as noise and blur caused by electromagnetic interference, electron counting errors, magnetic lens imperfections, electron diffraction, etc. These imperfections in raw image quality are inevitable and hamper subsequent image analysis and visualization. In an effort to mitigate these artefacts, many electron microscopy image restoration algorithms have been proposed in the last years. Most of these methods rely on generic assumptions on the image or degradations and are therefore outperformed by advanced methods that are based on more accurate models. Ideally, a method will accurately model the specific degradations that fit the physical acquisition settings. In this overview paper, we discuss different electron microscopy image degradation solutions and demonstrate that dedicated artefact regularisation results in higher quality restoration and is applicable through recently developed probabilistic methods. © 2018 The Authors Journal of Microscopy © 2018 Royal Microscopical Society.

Epipolar Rectification for CARTOSAT-1 Stereo Images Using SIFT and RANSAC

NASA Astrophysics Data System (ADS)

Akilan, A.; Sudheer Reddy, D.; Nagasubramanian, V.; Radhadevi, P. V.; Varadan, G.

2014-11-01

Cartosat-1 provides stereo images of spatial resolution 2.5 m with high fidelity of geometry. Stereo camera on the spacecraft has look angles of +26 degree and -5 degree respectively that yields effective along track stereo. Any DSM generation algorithm can use the stereo images for accurate 3D reconstruction and measurement of ground. Dense match points and pixel-wise matching are prerequisite in DSM generation to capture discontinuities and occlusions for accurate 3D modelling application. Epipolar image matching reduces the computational effort from two dimensional area searches to one dimensional. Thus, epipolar rectification is preferred as a pre-processing step for accurate DSM generation. In this paper we explore a method based on SIFT and RANSAC for epipolar rectification of cartosat-1 stereo images.

Multi-modal Registration for Correlative Microscopy using Image Analogies

PubMed Central

Cao, Tian; Zach, Christopher; Modla, Shannon; Powell, Debbie; Czymmek, Kirk; Niethammer, Marc

2014-01-01

Correlative microscopy is a methodology combining the functionality of light microscopy with the high resolution of electron microscopy and other microscopy technologies for the same biological specimen. In this paper, we propose an image registration method for correlative microscopy, which is challenging due to the distinct appearance of biological structures when imaged with different modalities. Our method is based on image analogies and allows to transform images of a given modality into the appearance-space of another modality. Hence, the registration between two different types of microscopy images can be transformed to a mono-modality image registration. We use a sparse representation model to obtain image analogies. The method makes use of corresponding image training patches of two different imaging modalities to learn a dictionary capturing appearance relations. We test our approach on backscattered electron (BSE) scanning electron microscopy (SEM)/confocal and transmission electron microscopy (TEM)/confocal images. We perform rigid, affine, and deformable registration via B-splines and show improvements over direct registration using both mutual information and sum of squared differences similarity measures to account for differences in image appearance. PMID:24387943

Hybrid fluorescence and electron cryo-microscopy for simultaneous electron and photon imaging.

PubMed

Iijima, Hirofumi; Fukuda, Yoshiyuki; Arai, Yoshihiro; Terakawa, Susumu; Yamamoto, Naoki; Nagayama, Kuniaki

2014-01-01

Integration of fluorescence light and transmission electron microscopy into the same device would represent an important advance in correlative microscopy, which traditionally involves two separate microscopes for imaging. To achieve such integration, the primary technical challenge that must be solved regards how to arrange two objective lenses used for light and electron microscopy in such a manner that they can properly focus on a single specimen. To address this issue, both lateral displacement of the specimen between two lenses and specimen rotation have been proposed. Such movement of the specimen allows sequential collection of two kinds of microscopic images of a single target, but prevents simultaneous imaging. This shortcoming has been made up by using a simple optical device, a reflection mirror. Here, we present an approach toward the versatile integration of fluorescence and electron microscopy for simultaneous imaging. The potential of simultaneous hybrid microscopy was demonstrated by fluorescence and electron sequential imaging of a fluorescent protein expressed in cells and cathodoluminescence imaging of fluorescent beads. Copyright © 2013 Elsevier Inc. All rights reserved.

[application of the analytical transmission electron microscopy techniques for detection, identification and visualization of localization of nanoparticles of titanium and cerium oxides in mammalian cells].

PubMed

Shebanova, A S; Bogdanov, A G; Ismagulova, T T; Feofanov, A V; Semenyuk, P I; Muronets, V I; Erokhina, M V; Onishchenko, G E; Kirpichnikov, M P; Shaitan, K V

2014-01-01

This work represents the results of the study on applicability of the modern methods of analytical transmission electron microscopy for detection, identification and visualization of localization of nanoparticles of titanium and cerium oxides in A549 cell, human lung adenocarcinoma cell line. A comparative analysis of images of the nanoparticles in the cells obtained in the bright field mode of transmission electron microscopy, under dark-field scanning transmission electron microscopy and high-angle annular dark field scanning transmission electron was performed. For identification of nanoparticles in the cells the analytical techniques, energy-dispersive X-ray spectroscopy and electron energy loss spectroscopy, were compared when used in the mode of obtaining energy spectrum from different particles and element mapping. It was shown that the method for electron tomography is applicable to confirm that nanoparticles are localized in the sample but not coated by contamination. The possibilities and fields of utilizing different techniques for analytical transmission electron microscopy for detection, visualization and identification of nanoparticles in the biological samples are discussed.

Chemistry Viewed through the Eyes of High-Resolution Microscopy.

ERIC Educational Resources Information Center

Beer, Michael; And Others

1981-01-01

This special report, prepared by several chemists working in the field of electron microscopy, provides information regarding the most recent developments in transmission and scanning electron microscopy that have chemical significance. (CS)

Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles.

PubMed

Burlaud-Gaillard, Julien; Sellin, Caroline; Georgeault, Sonia; Uzbekov, Rustem; Lebos, Claude; Guillaume, Jean-Marc; Roingeard, Philippe

2014-01-01

The intracellular morphogenesis of flaviviruses has been well described, but flavivirus release from the host cell remains poorly documented. We took advantage of the optimized production of an attenuated chimeric yellow fever/dengue virus for vaccine purposes to study this phenomenon by microscopic approaches. Scanning electron microscopy (SEM) showed the release of numerous viral particles at the cell surface through a short-lived process. For transmission electron microscopy (TEM) studies of the intracellular ultrastructure of the small number of cells releasing viral particles at a given time, we developed a new correlative microscopy method: CSEMTEM (for correlative scanning electron microscopy - transmission electron microscopy). CSEMTEM analysis suggested that chimeric flavivirus particles were released as individual particles, in small exocytosis vesicles, via a regulated secretory pathway. Our morphological findings provide new insight into interactions between flaviviruses and cells and demonstrate that CSEMTEM is a useful new method, complementary to SEM observations of biological events by intracellular TEM investigations.

Correlative Scanning-Transmission Electron Microscopy Reveals that a Chimeric Flavivirus Is Released as Individual Particles in Secretory Vesicles

PubMed Central

Burlaud-Gaillard, Julien; Sellin, Caroline; Georgeault, Sonia; Uzbekov, Rustem; Lebos, Claude; Guillaume, Jean-Marc; Roingeard, Philippe

2014-01-01

The intracellular morphogenesis of flaviviruses has been well described, but flavivirus release from the host cell remains poorly documented. We took advantage of the optimized production of an attenuated chimeric yellow fever/dengue virus for vaccine purposes to study this phenomenon by microscopic approaches. Scanning electron microscopy (SEM) showed the release of numerous viral particles at the cell surface through a short-lived process. For transmission electron microscopy (TEM) studies of the intracellular ultrastructure of the small number of cells releasing viral particles at a given time, we developed a new correlative microscopy method: CSEMTEM (for correlative scanning electron microscopy - transmission electron microscopy). CSEMTEM analysis suggested that chimeric flavivirus particles were released as individual particles, in small exocytosis vesicles, via a regulated secretory pathway. Our morphological findings provide new insight into interactions between flaviviruses and cells and demonstrate that CSEMTEM is a useful new method, complementary to SEM observations of biological events by intracellular TEM investigations. PMID:24681578

Simultaneous Correlative Scanning Electron and High-NA Fluorescence Microscopy

PubMed Central

Liv, Nalan; Zonnevylle, A. Christiaan; Narvaez, Angela C.; Effting, Andries P. J.; Voorneveld, Philip W.; Lucas, Miriam S.; Hardwick, James C.; Wepf, Roger A.; Kruit, Pieter; Hoogenboom, Jacob P.

2013-01-01

Correlative light and electron microscopy (CLEM) is a unique method for investigating biological structure-function relations. With CLEM protein distributions visualized in fluorescence can be mapped onto the cellular ultrastructure measured with electron microscopy. Widespread application of correlative microscopy is hampered by elaborate experimental procedures related foremost to retrieving regions of interest in both modalities and/or compromises in integrated approaches. We present a novel approach to correlative microscopy, in which a high numerical aperture epi-fluorescence microscope and a scanning electron microscope illuminate the same area of a sample at the same time. This removes the need for retrieval of regions of interest leading to a drastic reduction of inspection times and the possibility for quantitative investigations of large areas and datasets with correlative microscopy. We demonstrate Simultaneous CLEM (SCLEM) analyzing cell-cell connections and membrane protrusions in whole uncoated colon adenocarcinoma cell line cells stained for actin and cortactin with AlexaFluor488. SCLEM imaging of coverglass-mounted tissue sections with both electron-dense and fluorescence staining is also shown. PMID:23409024

LROC Stereo Observations

NASA Astrophysics Data System (ADS)

Beyer, Ross A.; Archinal, B.; Li, R.; Mattson, S.; Moratto, Z.; McEwen, A.; Oberst, J.; Robinson, M.

2009-09-01

The Lunar Reconnaissance Orbiter Camera (LROC) will obtain two types of multiple overlapping coverage to derive terrain models of the lunar surface. LROC has two Narrow Angle Cameras (NACs), working jointly to provide a wider (in the cross-track direction) field of view, as well as a Wide Angle Camera (WAC). LRO's orbit precesses, and the same target can be viewed at different solar azimuth and incidence angles providing the opportunity to acquire `photometric stereo' in addition to traditional `geometric stereo' data. Geometric stereo refers to images acquired by LROC with two observations at different times. They must have different emission angles to provide a stereo convergence angle such that the resultant images have enough parallax for a reasonable stereo solution. The lighting at the target must not be radically different. If shadows move substantially between observations, it is very difficult to correlate the images. The majority of NAC geometric stereo will be acquired with one nadir and one off-pointed image (20 degree roll). Alternatively, pairs can be obtained with two spacecraft rolls (one to the left and one to the right) providing a stereo convergence angle up to 40 degrees. Overlapping WAC images from adjacent orbits can be used to generate topography of near-global coverage at kilometer-scale effective spatial resolution. Photometric stereo refers to multiple-look observations of the same target under different lighting conditions. LROC will acquire at least three (ideally five) observations of a target. These observations should have near identical emission angles, but with varying solar azimuth and incidence angles. These types of images can be processed via various methods to derive single pixel resolution topography and surface albedo. The LROC team will produce some topographic models, but stereo data collection is focused on acquiring the highest quality data so that such models can be generated later.

Writing silica structures in liquid with scanning transmission electron microscopy.

PubMed

van de Put, Marcel W P; Carcouët, Camille C M C; Bomans, Paul H H; Friedrich, Heiner; de Jonge, Niels; Sommerdijk, Nico A J M

2015-02-04

Silica nanoparticles are imaged in solution with scanning transmission electron microscopy (STEM) using a liquid cell with silicon nitride (SiN) membrane windows. The STEM images reveal that silica structures are deposited in well-defined patches on the upper SiN membranes upon electron beam irradiation. The thickness of the deposits is linear with the applied electron dose. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) demonstrate that the deposited patches are a result of the merging of the original 20 nm-diameter nanoparticles, and that the related surface roughness depends on the electron dose rate used. Using this approach, sub-micrometer scale structures are written on the SiN in liquid by controlling the electron exposure as function of the lateral position. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Mastcam Stereo Analysis and Mosaics (MSAM)

NASA Astrophysics Data System (ADS)

Deen, R. G.; Maki, J. N.; Algermissen, S. S.; Abarca, H. E.; Ruoff, N. A.

2017-06-01

Describes a new PDART task that will generate stereo analysis products (XYZ, slope, etc.), terrain meshes, and mosaics (stereo, ortho, and Mast/Nav combos) for all MSL Mastcam images and deliver the results to PDS.

BRDF invariant stereo using light transport constancy.

PubMed

Wang, Liang; Yang, Ruigang; Davis, James E

2007-09-01

Nearly all existing methods for stereo reconstruction assume that scene reflectance is Lambertian and make use of brightness constancy as a matching invariant. We introduce a new invariant for stereo reconstruction called light transport constancy (LTC), which allows completely arbitrary scene reflectance (bidirectional reflectance distribution functions (BRDFs)). This invariant can be used to formulate a rank constraint on multiview stereo matching when the scene is observed by several lighting configurations in which only the lighting intensity varies. In addition, we show that this multiview constraint can be used with as few as two cameras and two lighting configurations. Unlike previous methods for BRDF invariant stereo, LTC does not require precisely configured or calibrated light sources or calibration objects in the scene. Importantly, the new constraint can be used to provide BRDF invariance to any existing stereo method whenever appropriate lighting variation is available.

Near real-time stereo vision system

NASA Technical Reports Server (NTRS)

Anderson, Charles H. (Inventor); Matthies, Larry H. (Inventor)

1993-01-01

The apparatus for a near real-time stereo vision system for use with a robotic vehicle is described. The system is comprised of two cameras mounted on three-axis rotation platforms, image-processing boards, a CPU, and specialized stereo vision algorithms. Bandpass-filtered image pyramids are computed, stereo matching is performed by least-squares correlation, and confidence ranges are estimated by means of Bayes' theorem. In particular, Laplacian image pyramids are built and disparity maps are produced from the 60 x 64 level of the pyramids at rates of up to 2 seconds per image pair. The first autonomous cross-country robotic traverses (of up to 100 meters) have been achieved using the stereo vision system of the present invention with all computing done onboard the vehicle. The overall approach disclosed herein provides a unifying paradigm for practical domain-independent stereo ranging.

X-ray microscopy as an approach to increasing accuracy and efficiency of serial block-face imaging for correlated light and electron microscopy of biological specimens.

PubMed

Bushong, Eric A; Johnson, Donald D; Kim, Keun-Young; Terada, Masako; Hatori, Megumi; Peltier, Steven T; Panda, Satchidananda; Merkle, Arno; Ellisman, Mark H

2015-02-01

The recently developed three-dimensional electron microscopic (EM) method of serial block-face scanning electron microscopy (SBEM) has rapidly established itself as a powerful imaging approach. Volume EM imaging with this scanning electron microscopy (SEM) method requires intense staining of biological specimens with heavy metals to allow sufficient back-scatter electron signal and also to render specimens sufficiently conductive to control charging artifacts. These more extreme heavy metal staining protocols render specimens light opaque and make it much more difficult to track and identify regions of interest (ROIs) for the SBEM imaging process than for a typical thin section transmission electron microscopy correlative light and electron microscopy study. We present a strategy employing X-ray microscopy (XRM) both for tracking ROIs and for increasing the efficiency of the workflow used for typical projects undertaken with SBEM. XRM was found to reveal an impressive level of detail in tissue heavily stained for SBEM imaging, allowing for the identification of tissue landmarks that can be subsequently used to guide data collection in the SEM. Furthermore, specific labeling of individual cells using diaminobenzidine is detectable in XRM volumes. We demonstrate that tungsten carbide particles or upconverting nanophosphor particles can be used as fiducial markers to further increase the precision and efficiency of SBEM imaging.

X-ray Microscopy as an Approach to Increasing Accuracy and Efficiency of Serial Block-face Imaging for Correlated Light and Electron Microscopy of Biological Specimens

PubMed Central

Bushong, Eric A.; Johnson, Donald D.; Kim, Keun-Young; Terada, Masako; Hatori, Megumi; Peltier, Steven T.; Panda, Satchidananda; Merkle, Arno; Ellisman, Mark H.

2015-01-01

The recently developed three-dimensional electron microscopic (EM) method of serial block-face scanning electron microscopy (SBEM) has rapidly established itself as a powerful imaging approach. Volume EM imaging with this scanning electron microscopy (SEM) method requires intense staining of biological specimens with heavy metals to allow sufficient back-scatter electron signal and also to render specimens sufficiently conductive to control charging artifacts. These more extreme heavy metal staining protocols render specimens light opaque and make it much more difficult to track and identify regions of interest (ROIs) for the SBEM imaging process than for a typical thin section transmission electron microscopy correlative light and electron microscopy study. We present a strategy employing X-ray microscopy (XRM) both for tracking ROIs and for increasing the efficiency of the workflow used for typical projects undertaken with SBEM. XRM was found to reveal an impressive level of detail in tissue heavily stained for SBEM imaging, allowing for the identification of tissue landmarks that can be subsequently used to guide data collection in the SEM. Furthermore, specific labeling of individual cells using diaminobenzidine is detectable in XRM volumes. We demonstrate that tungsten carbide particles or upconverting nanophosphor particles can be used as fiducial markers to further increase the precision and efficiency of SBEM imaging. PMID:25392009

Correlative cryo-fluorescence light microscopy and cryo-electron tomography of Streptomyces.

PubMed

Koning, Roman I; Celler, Katherine; Willemse, Joost; Bos, Erik; van Wezel, Gilles P; Koster, Abraham J

2014-01-01

Light microscopy and electron microscopy are complementary techniques that in a correlative approach enable identification and targeting of fluorescently labeled structures in situ for three-dimensional imaging at nanometer resolution. Correlative imaging allows electron microscopic images to be positioned in a broader temporal and spatial context. We employed cryo-correlative light and electron microscopy (cryo-CLEM), combining cryo-fluorescence light microscopy and cryo-electron tomography, on vitrified Streptomyces bacteria to study cell division. Streptomycetes are mycelial bacteria that grow as long hyphae and reproduce via sporulation. On solid media, Streptomyces subsequently form distinct aerial mycelia where cell division leads to the formation of unigenomic spores which separate and disperse to form new colonies. In liquid media, only vegetative hyphae are present divided by noncell separating crosswalls. Their multicellular life style makes them exciting model systems for the study of bacterial development and cell division. Complex intracellular structures have been visualized with transmission electron microscopy. Here, we describe the methods for cryo-CLEM that we applied for studying Streptomyces. These methods include cell growth, fluorescent labeling, cryo-fixation by vitrification, cryo-light microscopy using a Linkam cryo-stage, image overlay and relocation, cryo-electron tomography using a Titan Krios, and tomographic reconstruction. Additionally, methods for segmentation, volume rendering, and visualization of the correlative data are described. © 2014 Elsevier Inc. All rights reserved.

Precision Relative Positioning for Automated Aerial Refueling from a Stereo Imaging System

DTIC Science & Technology

2015-03-01

PRECISION RELATIVE POSITIONING FOR AUTOMATED AERIAL REFUELING FROM A STEREO IMAGING SYSTEM THESIS Kyle P. Werner, 2Lt, USAF AFIT-ENG-MS-15-M-048...REFUELING FROM A STEREO IMAGING SYSTEM THESIS Presented to the Faculty Department of Electrical and Computer Engineering Graduate School of...RELEASE; DISTRIBUTION UNLIMITED. AFIT-ENG-MS-15-M-048 PRECISION RELATIVE POSITIONING FOR AUTOMATED AERIAL REFUELING FROM A STEREO IMAGING SYSTEM THESIS

A comparison of static near stereo acuity in youth baseball/softball players and non-ball players.

PubMed

Boden, Lauren M; Rosengren, Kenneth J; Martin, Daniel F; Boden, Scott D

2009-03-01

Although many aspects of vision have been investigated in professional baseball players, few studies have been performed in developing athletes. The issue of whether youth baseball players have superior stereopsis to nonplayers has not been addressed specifically. The purpose of this study was to determine if youth baseball/softball players have better stereo acuity than non-ball players. Informed consent was obtained from 51 baseball/softball players and 52 non-ball players (ages 10 to 18 years). Subjects completed a questionnaire, and their static near stereo acuity was measured using the Randot Stereotest (Stereo Optical Company, Chicago, Illinois). Stereo acuity was measured as the seconds of arc between the last pair of images correctly distinguished by the subject. The mean stereo acuity score was 25.5 +/- 1.7 seconds of arc in the baseball/softball players and 56.2 +/- 8.4 seconds of arc in the non-ball players. This difference was statistically significant (P < 0.00001). In addition, a perfect stereo acuity score of 20 seconds of arc was seen in 61% of the ball players and only 23% of the non-ball players (P = 0.0001). Youth baseball/softball players had significantly better static stereo acuity than non-ball players, comparable to professional ball players.

Full-frame, high-speed 3D shape and deformation measurements using stereo-digital image correlation and a single color high-speed camera

NASA Astrophysics Data System (ADS)

Yu, Liping; Pan, Bing

2017-08-01

Full-frame, high-speed 3D shape and deformation measurement using stereo-digital image correlation (stereo-DIC) technique and a single high-speed color camera is proposed. With the aid of a skillfully designed pseudo stereo-imaging apparatus, color images of a test object surface, composed of blue and red channel images from two different optical paths, are recorded by a high-speed color CMOS camera. The recorded color images can be separated into red and blue channel sub-images using a simple but effective color crosstalk correction method. These separated blue and red channel sub-images are processed by regular stereo-DIC method to retrieve full-field 3D shape and deformation on the test object surface. Compared with existing two-camera high-speed stereo-DIC or four-mirror-adapter-assisted singe-camera high-speed stereo-DIC, the proposed single-camera high-speed stereo-DIC technique offers prominent advantages of full-frame measurements using a single high-speed camera but without sacrificing its spatial resolution. Two real experiments, including shape measurement of a curved surface and vibration measurement of a Chinese double-side drum, demonstrated the effectiveness and accuracy of the proposed technique.

A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics

PubMed Central

Tacke, Sebastian; Krzyzanek, Vladislav; Nüsse, Harald; Wepf, Roger Albert; Klingauf, Jürgen; Reichelt, Rudolf

2016-01-01

Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented. PMID:26910419

The New Electron Microscopy: Cells and Molecules in Three Dimensions | Poster

Cancer.gov

NCI recently announced the launch of the new National Cryo-Electron Microscopy Facility (NCEF) at the Frederick National Laboratory for Cancer Research (FNLCR). The launch comes while cryo-electron microscopy (cryo-EM) is enjoying the spotlight as a newly emerging, rapidly evolving technology with the potential to revolutionize the field of structural biology. Read more...

Crystal structure of stacking faults in InGaAs/InAlAs/InAs heterostructures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Trunkin, I. N.; Presniakov, M. Yu.; Vasiliev, A. L., E-mail: a.vasiliev56@gmail.com

Stacking faults and dislocations in InGaAs/InAlAs/InAs heterostructures have been studied by electron microscopy. The use of different techniques of transmission electron microscopy (primarily, highresolution dark-field scanning transmission electron microscopy) has made it possible to determine the defect structure at the atomic level.

Near-infrared branding efficiently correlates light and electron microscopy.

PubMed

Bishop, Derron; Nikić, Ivana; Brinkoetter, Mary; Knecht, Sharmon; Potz, Stephanie; Kerschensteiner, Martin; Misgeld, Thomas

2011-06-05

The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.

Stereo depth distortions in teleoperation

NASA Technical Reports Server (NTRS)

Diner, Daniel B.; Vonsydow, Marika

1988-01-01

In teleoperation, a typical application of stereo vision is to view a work space located short distances (1 to 3m) in front of the cameras. The work presented here treats converged camera placement and studies the effects of intercamera distance, camera-to-object viewing distance, and focal length of the camera lenses on both stereo depth resolution and stereo depth distortion. While viewing the fronto-parallel plane 1.4 m in front of the cameras, depth errors are measured on the order of 2cm. A geometric analysis was made of the distortion of the fronto-parallel plane of divergence for stereo TV viewing. The results of the analysis were then verified experimentally. The objective was to determine the optimal camera configuration which gave high stereo depth resolution while minimizing stereo depth distortion. It is found that for converged cameras at a fixed camera-to-object viewing distance, larger intercamera distances allow higher depth resolutions, but cause greater depth distortions. Thus with larger intercamera distances, operators will make greater depth errors (because of the greater distortions), but will be more certain that they are not errors (because of the higher resolution).

Neuroanatomy from Mesoscopic to Nanoscopic Scales: An Improved Method for the Observation of Semithin Sections by High-Resolution Scanning Electron Microscopy

PubMed Central

Rodríguez, José-Rodrigo; Turégano-López, Marta; DeFelipe, Javier; Merchán-Pérez, Angel

2018-01-01

Semithin sections are commonly used to examine large areas of tissue with an optical microscope, in order to locate and trim the regions that will later be studied with the electron microscope. Ideally, the observation of semithin sections would be from mesoscopic to nanoscopic scales directly, instead of using light microscopy and then electron microscopy (EM). Here we propose a method that makes it possible to obtain high-resolution scanning EM images of large areas of the brain in the millimeter to nanometer range. Since our method is compatible with light microscopy, it is also feasible to generate hybrid light and electron microscopic maps. Additionally, the same tissue blocks that have been used to obtain semithin sections can later be used, if necessary, for transmission EM, or for focused ion beam milling and scanning electron microscopy (FIB-SEM). PMID:29568263

Neuroanatomy from Mesoscopic to Nanoscopic Scales: An Improved Method for the Observation of Semithin Sections by High-Resolution Scanning Electron Microscopy.

PubMed

Rodríguez, José-Rodrigo; Turégano-López, Marta; DeFelipe, Javier; Merchán-Pérez, Angel

2018-01-01

Semithin sections are commonly used to examine large areas of tissue with an optical microscope, in order to locate and trim the regions that will later be studied with the electron microscope. Ideally, the observation of semithin sections would be from mesoscopic to nanoscopic scales directly, instead of using light microscopy and then electron microscopy (EM). Here we propose a method that makes it possible to obtain high-resolution scanning EM images of large areas of the brain in the millimeter to nanometer range. Since our method is compatible with light microscopy, it is also feasible to generate hybrid light and electron microscopic maps. Additionally, the same tissue blocks that have been used to obtain semithin sections can later be used, if necessary, for transmission EM, or for focused ion beam milling and scanning electron microscopy (FIB-SEM).

Advantages of indium-tin oxide-coated glass slides in correlative scanning electron microscopy applications of uncoated cultured cells.

PubMed

Pluk, H; Stokes, D J; Lich, B; Wieringa, B; Fransen, J

2009-03-01

A method of direct visualization by correlative scanning electron microscopy (SEM) and fluorescence light microscopy of cell structures of tissue cultured cells grown on conductive glass slides is described. We show that by growing cells on indium-tin oxide (ITO)-coated glass slides, secondary electron (SE) and backscatter electron (BSE) images of uncoated cells can be obtained in high-vacuum SEM without charging artefacts. Interestingly, we observed that BSE imaging is influenced by both accelerating voltage and ITO coating thickness. By combining SE and BSE imaging with fluorescence light microscopy imaging, we were able to reveal detailed features of actin cytoskeletal and mitochondrial structures in mouse embryonic fibroblasts. We propose that the application of ITO glass as a substrate for cell culture can easily be extended and offers new opportunities for correlative light and electron microscopy studies of adherently growing cells.

Retraction: Using the Medipix3 detector for direct electron imaging in the range 60 keV to 200 keV in electron microscopy Retraction: Using the Medipix3 detector for direct electron imaging in the range 60 keV to 200 keV in electron microscopy

NASA Astrophysics Data System (ADS)

Mir, J. A.; Plackett, R.; Shipsey, I.; dos Santos, J. M. F.

2018-01-01

The paper "Using the Medipix3 detector for direct electron imaging in the range 60keV to 200keV in electron microscopy" by J.A. Mir, R. Plackett, I. Shipsey and J.M.F. dos Santos has been retracted following the authors' request on the basis of the existence of a disagreement about the ownership of the data, to prevent conflict between collaborators.

Spatial Resolution in Scanning Electron Microscopy and Scanning Transmission Electron Microscopy Without a Specimen Vacuum Chamber.

PubMed

Nguyen, Kayla X; Holtz, Megan E; Richmond-Decker, Justin; Muller, David A

2016-08-01

A long-standing goal of electron microscopy has been the high-resolution characterization of specimens in their native environment. However, electron optics require high vacuum to maintain an unscattered and focused probe, a challenge for specimens requiring atmospheric or liquid environments. Here, we use an electron-transparent window at the base of a scanning electron microscope's objective lens to separate column vacuum from the specimen, enabling imaging under ambient conditions, without a specimen vacuum chamber. We demonstrate in-air imaging of specimens at nanoscale resolution using backscattered scanning electron microscopy (airSEM) and scanning transmission electron microscopy. We explore resolution and contrast using Monte Carlo simulations and analytical models. We find that nanometer-scale resolution can be obtained at gas path lengths up to 400 μm, although contrast drops with increasing gas path length. As the electron-transparent window scatters considerably more than gas at our operating conditions, we observe that the densities and thicknesses of the electron-transparent window are the dominant limiting factors for image contrast at lower operating voltages. By enabling a variety of detector configurations, the airSEM is applicable to a wide range of environmental experiments including the imaging of hydrated biological specimens and in situ chemical and electrochemical processes.

Spatial Resolution in Scanning Electron Microscopy and Scanning Transmission Electron Microscopy Without a Specimen Vacuum Chamber

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nguyen, Kayla X.; Holtz, Megan E.; Richmond-Decker, Justin

2016-07-25

Abstract A long-standing goal of electron microscopy has been the high-resolution characterization of specimens in their native environment. However, electron optics require high vacuum to maintain an unscattered and focused probe, a challenge for specimens requiring atmospheric or liquid environments. Here, we use an electron-transparent window at the base of a scanning electron microscope’s objective lens to separate column vacuum from the specimen, enabling imaging under ambient conditions, without a specimen vacuum chamber. We demonstrate in-air imaging of specimens at nanoscale resolution using backscattered scanning electron microscopy (airSEM) and scanning transmission electron microscopy. We explore resolution and contrast using Montemore » Carlo simulations and analytical models. We find that nanometer-scale resolution can be obtained at gas path lengths up to 400μm, although contrast drops with increasing gas path length. As the electron-transparent window scatters considerably more than gas at our operating conditions, we observe that the densities and thicknesses of the electron-transparent window are the dominant limiting factors for image contrast at lower operating voltages. By enabling a variety of detector configurations, the airSEM is applicable to a wide range of environmental experiments including the imaging of hydrated biological specimens andin situchemical and electrochemical processes.« less

The Effect of Electron Beam Irradiation in Environmental Scanning Transmission Electron Microscopy of Whole Cells in Liquid.

PubMed

Hermannsdörfer, Justus; Tinnemann, Verena; Peckys, Diana B; de Jonge, Niels

2016-06-01

Whole cells can be studied in their native liquid environment using electron microscopy, and unique information about the locations and stoichiometry of individual membrane proteins can be obtained from many cells thus taking cell heterogeneity into account. Of key importance for the further development of this microscopy technology is knowledge about the effect of electron beam radiation on the samples under investigation. We used environmental scanning electron microscopy (ESEM) with scanning transmission electron microscopy (STEM) detection to examine the effect of radiation for whole fixed COS7 fibroblasts in liquid. The main observation was the localization of nanoparticle labels attached to epidermal growth factor receptors (EGFRs). It was found that the relative distances between the labels remained mostly unchanged (<1.5%) for electron doses ranging from the undamaged native state at 10 e-/Å2 toward 103 e-/Å2. This dose range was sufficient to determine the EGFR locations with nanometer resolution and to distinguish between monomers and dimers. Various different forms of radiation damage became visible at higher doses, including severe dislocation, and the dissolution of labels.

Three-dimensional visualization and display technologies; Proceedings of the Meeting, Los Angeles, CA, Jan. 18-20, 1989

NASA Technical Reports Server (NTRS)

Robbins, Woodrow E. (Editor); Fisher, Scott S. (Editor)

1989-01-01

Special attention was given to problems of stereoscopic display devices, such as CAD for enhancement of the design process in visual arts, stereo-TV improvement of remote manipulator performance, a voice-controlled stereographic video camera system, and head-mounted displays and their low-cost design alternatives. Also discussed was a novel approach to chromostereoscopic microscopy, computer-generated barrier-strip autostereography and lenticular stereograms, and parallax barrier three-dimensional TV. Additional topics include processing and user interface isssues and visualization applications, including automated analysis and fliud flow topology, optical tomographic measusrements of mixing fluids, visualization of complex data, visualization environments, and visualization management systems.

KSC-06pd2272

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - Inside the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, workers check the clearance of the STEREO spacecraft as it is moved away from the opening. In the tower, STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2266

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - On Launch Pad 17-B at Cape Canaveral Air Force Station, the STEREO spacecraft is lifted off its transporter alongside the mobile service tower. In the tower, STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2268

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - Against a pre-dawn sky on Launch Pad 17-B at Cape Canaveral Air Force Station, the STEREO spacecraft is lifted up toward the platform on the mobile service tower. In the tower, STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2269

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - Viewed from inside the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, workers watch the progress of the STEREO spacecraft being lifted. Once in the tower, STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2270

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - On Launch Pad 17-B at Cape Canaveral Air Force Station, workers begin maneuvering the STEREO spacecraft into the mobile service tower. Once in the tower, STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2271

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - On Launch Pad 17-B at Cape Canaveral Air Force Station, workers observe the progress of the STEREO spacecraft as it glides inside the mobile service tower. After it is in the tower, STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2267

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - Against a pre-dawn sky on Launch Pad 17-B at Cape Canaveral Air Force Station, the STEREO spacecraft is lifted alongside the mobile service tower. In the tower, STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2264

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - After arriving at Launch Pad 17-B on Cape Canaveral Air Force Station, the STEREO spacecraft waits for a crane to be fitted over it and be lifted into the mobile service tower. STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2265

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - After arriving at Launch Pad 17-B on Cape Canaveral Air Force Station, the STEREO spacecraft is fitted with a crane to lift it into the mobile service tower. STEREO will be mated with its launch vehicle, a Boeing Delta II rocket. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

A 3D terrain reconstruction method of stereo vision based quadruped robot navigation system

NASA Astrophysics Data System (ADS)

Ge, Zhuo; Zhu, Ying; Liang, Guanhao

2017-01-01

To provide 3D environment information for the quadruped robot autonomous navigation system during walking through rough terrain, based on the stereo vision, a novel 3D terrain reconstruction method is presented. In order to solve the problem that images collected by stereo sensors have large regions with similar grayscale and the problem that image matching is poor at real-time performance, watershed algorithm and fuzzy c-means clustering algorithm are combined for contour extraction. Aiming at the problem of error matching, duel constraint with region matching and pixel matching is established for matching optimization. Using the stereo matching edge pixel pairs, the 3D coordinate algorithm is estimated according to the binocular stereo vision imaging model. Experimental results show that the proposed method can yield high stereo matching ratio and reconstruct 3D scene quickly and efficiently.

Orbit Determination and Navigation of the Solar Terrestrial Relations Observatory (STEREO)

NASA Technical Reports Server (NTRS)

Mesarch, Michael A.; Robertson, Mika; Ottenstein, Neil; Nicholson, Ann; Nicholson, Mark; Ward, Douglas T.; Cosgrove, Jennifer; German, Darla; Hendry, Stephen; Shaw, James

2007-01-01

This paper provides an overview of the required upgrades necessary for navigation of NASA's twin heliocentric science missions, Solar TErestrial RElations Observatory (STEREO) Ahead and Behind. The orbit determination of the STEREO spacecraft was provided by the NASA Goddard Space Flight Center's (GSFC) Flight Dynamics Facility (FDF) in support of the mission operations activities performed by the Johns Hopkins University Applied Physics Laboratory (APL). The changes to FDF's orbit determination software included modeling upgrades as well as modifications required to process the Deep Space Network X-band tracking data used for STEREO. Orbit results as well as comparisons to independently computed solutions are also included. The successful orbit determination support aided in maneuvering the STEREO spacecraft, launched on October 26, 2006 (00:52 Z), to target the lunar gravity assists required to place the spacecraft into their final heliocentric drift-away orbits where they are providing stereo imaging of the Sun.

Orbit Determination and Navigation of the Solar Terrestrial Relations Observatory (STEREO)

NASA Technical Reports Server (NTRS)

Mesarch, Michael; Robertson, Mika; Ottenstein, Neil; Nicholson, Ann; Nicholson, Mark; Ward, Douglas T.; Cosgrove, Jennifer; German, Darla; Hendry, Stephen; Shaw, James

2007-01-01

This paper provides an overview of the required upgrades necessary for navigation of NASA's twin heliocentric science missions, Solar TErestrial RElations Observatory (STEREO) Ahead and Behind. The orbit determination of the STEREO spacecraft was provided by the NASA Goddard Space Flight Center's (GSFC) Flight Dynamics Facility (FDF) in support of the mission operations activities performed by the Johns Hopkins University Applied Physics Laboratory (APL). The changes to FDF s orbit determination software included modeling upgrades as well as modifications required to process the Deep Space Network X-band tracking data used for STEREO. Orbit results as well as comparisons to independently computed solutions are also included. The successful orbit determination support aided in maneuvering the STEREO spacecraft, launched on October 26, 2006 (00:52 Z), to target the lunar gravity assists required to place the spacecraft into their final heliocentric drift-away orbits where they are providing stereo imaging of the Sun.

Chapter 14: Electron Microscopy on Thin Films for Solar Cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Romero, Manuel; Abou-Ras, Daniel; Nichterwitz, Melanie

2016-07-22

This chapter overviews the various techniques applied in scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and highlights their possibilities and also limitations. It gives the various imaging and analysis techniques applied on a scanning electron microscope. The chapter shows that imaging is divided into that making use of secondary electrons (SEs) and of backscattered electrons (BSEs), resulting in different contrasts in the images and thus providing information on compositions, microstructures, and surface potentials. Whenever aiming for imaging and analyses at scales of down to the angstroms range, TEM and its related techniques are appropriate tools. In many cases,more » also SEM techniques provide the access to various material properties of the individual layers, not requiring specimen preparation as time consuming as TEM techniques. Finally, the chapter dedicates to cross-sectional specimen preparation for electron microscopy. The preparation decides indeed on the quality of imaging and analyses.« less

Electron microscopy methods in studies of cultural heritage sites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vasiliev, A. L., E-mail: a.vasiliev56@gmail.com; Kovalchuk, M. V.; Yatsishina, E. B.

The history of the development and application of scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray microanalysis (EDXMA) in studies of cultural heritage sites is considered. In fact, investigations based on these methods began when electron microscopes became a commercial product. Currently, these methods, being developed and improved, help solve many historical enigmas. To date, electron microscopy combined with microanalysis makes it possible to investigate any object, from parchment and wooden articles to pigments, tools, and objects of art. Studies by these methods have revealed that some articles were made by ancient masters using ancient “nanotechnologies”; hence,more » their comprehensive analysis calls for the latest achievements in the corresponding instrumental methods and sample preparation techniques.« less

Electron microscopy methods in studies of cultural heritage sites

NASA Astrophysics Data System (ADS)

Vasiliev, A. L.; Kovalchuk, M. V.; Yatsishina, E. B.

2016-11-01

The history of the development and application of scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray microanalysis (EDXMA) in studies of cultural heritage sites is considered. In fact, investigations based on these methods began when electron microscopes became a commercial product. Currently, these methods, being developed and improved, help solve many historical enigmas. To date, electron microscopy combined with microanalysis makes it possible to investigate any object, from parchment and wooden articles to pigments, tools, and objects of art. Studies by these methods have revealed that some articles were made by ancient masters using ancient "nanotechnologies"; hence, their comprehensive analysis calls for the latest achievements in the corresponding instrumental methods and sample preparation techniques.

A scanning electron microscopy study of the macro-crystalline structure of 2-(2,4-dinitrobenzyl) pyridine

NASA Technical Reports Server (NTRS)

Ware, Jacqueline; Hammond, Ernest C., Jr.

1989-01-01

The compound, 2-(2,4-dinitrobenzyl) pyridine, was synthesized in the laboratory; an introductory level electron microscopy study of the macro-crystalline structure was conducted using the scanning electron microscope (SEM). The structure of these crystals was compared with the macrostructure of the crystal of 2-(2,4-dinitrobenzyl) pyridinium bromide, the hydrobromic salt of the compound which was also synthesized in the laboratory. A scanning electron microscopy crystal study was combined with a study of the principle of the electron microscope.

Microscopy and microanalysis 1996

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bailey, G.W.; Corbett, J.M.; Dimlich, R.V.W.

1996-12-31

The Proceedings of this Annual Meeting contain paper of members from the three societies. These proceedings emphasizes the common research interests and attempts to eliminate some unwanted overlap. Topics covered are: microscopic analysis of animals with altered gene expression and in-situ gene and antibody localizations, high-resolution elemental mapping of nucleoprofein interactions, plant biology and pathology, quantitative HREM analysis of perfect and defected materials, computational methods for TEM image analysis, high-resolution FESM in materials research, frontiers in polymer microscopy and microanalysis, oxidation and corrosion, micro XRD and XRF, molecular microspectroscopy and spectral imaging, advances in confocal and multidimensional light microscopy, analyticalmore » electron microscopy in biology, correlative microscopy in biological sciences, grain-boundary microengineering, surfaces and interfaces, telepresence microscopy in education and research, MSA educational outreach, quantitative electron probe microanalysis, frontiers of analytical electron microscopy, critical issues in ceramic microstructures, dynamic organization of the cell, pathology, microbiology, high-resolution biological and cryo SEM, and scanning-probe microscopy.« less

Forest abovegroundbiomass mapping using spaceborne stereo imagery acquired by Chinese ZY-3

NASA Astrophysics Data System (ADS)

Sun, G.; Ni, W.; Zhang, Z.; Xiong, C.

2015-12-01

Besides LiDAR data, another valuable type of data which is also directly sensitive to forest vertical structures and more suitable for regional mapping of forest biomass is the stereo imagery or photogrammetry. Photogrammetry is the traditional technique for deriving terrain elevation. The elevation of the top of a tree canopy can be directly measured from stereo imagery but winter images are required to get the elevation of ground surface because stereo images are acquired by optical sensors which cannot penetrate dense forest canopies with leaf-on condition. Several spaceborne stereoscopic systems with higher spatial resolutions have been launched in the past several years. For example the Chinese satellite Zi Yuan 3 (ZY-3) specifically designed for the collection of stereo imagery with a resolution of 3.6 m for forward and backward views and 2.1 m for the nadir view was launched on January 9, 2012. Our previous studies have demonstrated that the spaceborne stereo imagery acquired in summer has good performance on the description of forest structures. The ground surface elevation could be extracted from spaceborne stereo imagery acquired in winter. This study mainly focused on assessing the mapping of forest biomass through the combination of spaceborne stereo imagery acquired in summer and those in winter. The test sites of this study located at Daxing AnlingMountains areas as shown in Fig.1. The Daxing Anling site is on the south border of boreal forest belonging to frigid-temperate zone coniferous forest vegetation The dominant tree species is Dhurian larch (Larix gmelinii). 10 scenes of ZY-3 stereo images are used in this study. 5 scenes were acquired on March 14,2012 while the other 5 scenes were acquired on September 7, 2012. Their spatial coverage is shown in Fig.2-a. Fig.2-b is the mosaic of nadir images acquired on 09/07/2012 while Fig.2-c is thecorresponding digital surface model (DSM) derived from stereo images acquired on 09/07/2012. Fig.2-d is the difference between the DSM derived from stereo imagery acquired on 09/07/2012 and the digital elevation model (DEM) from stereo imagery acquired on 03/14/2012.The detailed analysis will be given in the final report.

A Small Lunar Rover for Reconnaissance in the Framework of ExoGeoLab Project, System Level Design

NASA Astrophysics Data System (ADS)

Noroozi, A.; Ha, L.; van Dalen, P.; Maas, A.; de Raedt, S.; Poulakis, P.; Foing, B. H.

2009-04-01

Scientific research is based on accurate measurement and so depends on the possibilities of accurate instruments. In planetary science and exploration it is often difficult or even impossible in some cases to gather accurate and direct information from a specified target. It is important to gather as much information as possible to be able to analyze and extract scientific data from them. One possibility to do so is to send equipments to the target and perform the measurements locally. The measurement data is then sent to base station for further analysis. To send measurement instruments to measurement point it is important to have a good estimation of the environmental situation there. This information can be collected by sending a pilot rover to the area of interest to collect visual information. The aim of this work is to develop a tele-operated small rover, Google Lunar X-Prize (GLXP) class, which is capable of surviving in the Moon environment and perform reconnaissance to provide visual information to base station of ExoGeoLab project of ESA/ESTEC. Using the state of the art developments in electronics, software and communication technologies allows us to achieve increase in accuracy while reducing size and power consumption. Target mass of the rover is lees than 5 kg and its target dimension is 300 x 60 x 80 mm3. The small size of the rover gives the possibility of accessing places which are normally out of reach. The required power for operation and the cost of launch is considerably reduced compared to large rovers which makes the mission more cost effective. The mission of the rover is to capture high resolution images and transmit them to base station. Data link between lover and base station is wireless and rover should supply its own energy. The base station can be either a habitat or a relay station. The navigation of the rover is controlled by an operator in a habitat who has a view from the stereo camera on the rover. This stereo camera gives image information to the base and gives the possibility for future autonomous navigation by using three-dimensional image recognition software. As the navigation view should have minimum delay, the resolution of stereo camera is not very high. The rover design is divided into four work packages. These work packages are remote imaging, remote manual navigation, locomotion and structure, and power system. Remote imaging work package is responsible for capturing high resolution images, transmitting image data to base station via wireless link and store the data for further processing. Remote manual navigation is handling the tele-operation. It collects stereo images and navigation sensor readouts, transmits stereo images and navigation data to base station via wireless link, displays the image and sensor status in a real-time fashion on operator's monitor, receives command from operator's joystick, transfers navigation commands to rover via wireless link, and operates the actuators accordingly. Locomotion and structure takes care of designing the body structure and locomotion system based on the Moon environment specifications. The target specifications of rover locomotion system are maximum speed of 200 m/h, maximum acceleration of 0.554 m/s2, and maximum slope angle of 20˚ . The power system for the rover includes the solar panel, batteries and power electronics mounted on the rover. The energy storage in the rover should be able to survive for minimum 500 m movement on the moon. Subsequently, it should provide energy for other sub-systems to communicate, navigate and transmit the data. Considering the harsh environmental issues on the Moon such as dust, temperature range and radiation, it is vital for the mission that these issues are considered in the design to correctly dimension reliability and if necessary redundancy. Corrosion resistive material should be used to ensure the survival of mechanical structure, moving parts and other sensitive parts such as electronics. High temperature variation should be considered in the design of structure and electronics and finally electronics should be radiation protected.

STEREO Mission Design

NASA Technical Reports Server (NTRS)

Dunham, David W.; Guzman, Jose J.; Sharer, Peter J.; Friessen, Henry D.

2007-01-01

STEREO (Solar-TErestrial RElations Observatory) is the third mission in the Solar Terrestrial Probes program (STP) of the National Aeronautics and Space Administration (NASA). STEREO is the first mission to utilize phasing loops and multiple lunar flybys to alter the trajectories of more than one satellite. This paper describes the launch computation methodology, the launch constraints, and the resulting nine launch windows that were prepared for STEREO. More details are provided for the window in late October 2006 that was actually used.

Stereo Science Update

NASA Image and Video Library

2009-04-13

Michael Kaiser, project scientist, Solar Terrestrial Relations Observatory (STEREO) at Goddard Space Flight Center, left, makes a point during a Science Update on the STEREO mission at NASA Headquarters in Washington, Tuesday, April 14, 2009, as Angelo Vourlidas, project scientist, Sun Earth Connection Coronal and Heliospheric Investigation, at the Naval Research Laboratory, Toni Galvin, principal investigator, Plasma and Superthermal Ion Composition instrument at the University of New Hampshire and Madhulika Guhathkurta, STEREO program scientist, right, look on. Photo Credit: (NASA/Paul E. Alers)

KSC-06pd1153

NASA Image and Video Library

2006-06-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., technicians check the STEREO spacecraft "B" is secure on the stand. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket on July 22. Photo credit: NASA/George Shelton

Deblocking of mobile stereo video

NASA Astrophysics Data System (ADS)

Azzari, Lucio; Gotchev, Atanas; Egiazarian, Karen

2012-02-01

Most of candidate methods for compression of mobile stereo video apply block-transform based compression based on the H-264 standard with quantization of transform coefficients driven by quantization parameter (QP). The compression ratio and the resulting bit rate are directly determined by the QP level and high compression is achieved for the price of visually noticeable blocking artifacts. Previous studies on perceived quality of mobile stereo video have revealed that blocking artifacts are the most annoying and most influential in the acceptance/rejection of mobile stereo video and can even completely cancel the 3D effect and the corresponding quality added value. In this work, we address the problem of deblocking of mobile stereo video. We modify a powerful non-local transform-domain collaborative filtering method originally developed for denoising of images and video. The method employs grouping of similar block patches residing in spatial and temporal vicinity of a reference block in filtering them collaboratively in a suitable transform domain. We study the most suitable way of finding similar patches in both channels of stereo video and suggest a hybrid four-dimensional transform to process the collected synchronized (stereo) volumes of grouped blocks. The results benefit from the additional correlation available between the left and right channel of the stereo video. Furthermore, addition sharpening is applied through an embedded alpha-rooting in transform domain, which improve the visual appearance of the deblocked frames.

Tissue and cellular localization of tannins in Tunisian dates (Phoenix dactylifera L.) by light and transmission electron microscopy.

PubMed

Hammouda, Hédi; Alvarado, Camille; Bouchet, Brigitte; Kalthoum-Chérif, Jamila; Trabelsi-Ayadi, Malika; Guyot, Sylvain

2014-07-16

A histological approach including light microscopy and transmission electron microscopy (TEM) was used to provide accurate information on the localization of condensed tannins in the edible tissues and in the stone of date fruits (Phoenix dactylifera L.). Light microscopy was carried out on fresh tissues after staining by 4-dimethylaminocinnamaldehyde (DMACA) for a specific detection of condensed tannins. Thus, whether under light microscopy or transmission electron microscopy (TEM), results showed that tannins are not located in the epidermis but more deeply in the mesocarp in the vacuole of very large cells. Regarding the stones, tannins are found in a specific cell layer located at 50 μm from the sclereid cells of the testa.

Liquid scanning transmission electron microscopy: imaging protein complexes in their native environment in whole eukaryotic cells.

PubMed

Peckys, Diana B; de Jonge, Niels

2014-04-01

Scanning transmission electron microscopy (STEM) of specimens in liquid, so-called Liquid STEM, is capable of imaging the individual subunits of macromolecular complexes in whole eukaryotic cells in liquid. This paper discusses this new microscopy modality within the context of state-of-the-art microscopy of cells. The principle of operation and equations for the resolution are described. The obtained images are different from those acquired with standard transmission electron microscopy showing the cellular ultrastructure. Instead, contrast is obtained on specific labels. Images can be recorded in two ways, either via STEM at 200 keV electron beam energy using a microfluidic chamber enclosing the cells, or via environmental scanning electron microscopy at 30 keV of cells in a wet environment. The first series of experiments involved the epidermal growth factor receptor labeled with gold nanoparticles. The labels were imaged in whole fixed cells with nanometer resolution. Since the cells can be kept alive in the microfluidic chamber, it is also feasible to detect the labels in unfixed, live cells. The rapid sample preparation and imaging allows studies of multiple whole cells.

Advanced Nanoscale Thin Film & Bulk Materials Towards Thermoelectric Power Conversion Efficiencies of 30%

DTIC Science & Technology

2014-02-27

Electron Microscopy. Detailed Kronig -Penny (K-P)) modeling of electron transport through these superlattices suggests an estimated e-h transition energy...superalttices was confirmed by Transmission Electron Microscopy. Detailed Kronig -Penny (K-P)) modeling of electron transport through these superlattices

New modes of electron microscopy for materials science enabled by fast direct electron detectors

NASA Astrophysics Data System (ADS)

Minor, Andrew

There is an ongoing revolution in the development of electron detector technology that has enabled modes of electron microscopy imaging that had only before been theorized. The age of electron microscopy as a tool for imaging is quickly giving way to a new frontier of multidimensional datasets to be mined. These improvements in electron detection have enabled cryo-electron microscopy to resolve the three-dimensional structures of non-crystalized proteins, revolutionizing structural biology. In the physical sciences direct electron detectors has enabled four-dimensional reciprocal space maps of materials at atomic resolution, providing all the structural information about nanoscale materials in one experiment. This talk will highlight the impact of direct electron detectors for materials science, including a new method of scanning nanobeam diffraction. With faster detectors we can take a series of 2D diffraction patterns at each position in a 2D STEM raster scan resulting in a four-dimensional data set. For thin film analysis, direct electron detectors hold the potential to enable strain, polarization, composition and electrical field mapping over relatively large fields of view, all from a single experiment.

Local Surface Reconstruction from MER images using Stereo Workstation

NASA Astrophysics Data System (ADS)

Shin, Dongjoe; Muller, Jan-Peter

2010-05-01

The authors present a semi-automatic workflow that reconstructs the 3D shape of the martian surface from local stereo images delivered by PnCam or NavCam on systems such as the NASA Mars Exploration Rover (MER) Mission and in the future the ESA-NASA ExoMars rover PanCam. The process is initiated with manually selected tiepoints on a stereo workstation which is then followed by a tiepoint refinement, stereo-matching using region growing and Levenberg-Marquardt Algorithm (LMA)-based bundle adjustment processing. The stereo workstation, which is being developed by UCL in collaboration with colleagues at the Jet Propulsion Laboratory (JPL) within the EU FP7 ProVisG project, includes a set of practical GUI-based tools that enable an operator to define a visually correct tiepoint via a stereo display. To achieve platform and graphic hardware independence, the stereo application has been implemented using JPL's JADIS graphic library which is written in JAVA and the remaining processing blocks used in the reconstruction workflow have also been developed as a JAVA package to increase the code re-usability, portability and compatibility. Although initial tiepoints from the stereo workstation are reasonably acceptable as true correspondences, it is often required to employ an optional validity check and/or quality enhancing process. To meet this requirement, the workflow has been designed to include a tiepoint refinement process based on the Adaptive Least Square Correlation (ALSC) matching algorithm so that the initial tiepoints can be further enhanced to sub-pixel precision or rejected if they fail to pass the ALSC matching threshold. Apart from the accuracy of reconstruction, it is obvious that the other criterion to assess the quality of reconstruction is the density (or completeness) of reconstruction, which is not attained in the refinement process. Thus, we re-implemented a stereo region growing process, which is a core matching algorithm within the UCL-HRSC reconstruction workflow. This algorithm's performance is reasonable even for close-range imagery so long as the stereo -pair does not too large a baseline displacement. For post-processing, a Bundle Adjustment (BA) is used to optimise the initial calibration parameters, which bootstrap the reconstruction results. Amongst many options for the non-linear optimisation, the LMA has been adopted due to its stability so that the BA searches the best calibration parameters whilst iteratively minimising the re-projection errors of the initial reconstruction points. For the evaluation of the proposed method, the result of the method is compared with the reconstruction from a disparity map provided by JPL using their operational processing system. Visual and quantitative comparison will be presented as well as updated camera parameters. As part of future work, we will investigate a method expediting the processing speed of the stereo region growing process and look into the possibility of extending the use of the stereo workstation to orbital image processing. Such an interactive stereo workstation can also be used to digitize points and line features as well as assess the accuracy of stereo processed results produced from other stereo matching algorithms available from within the consortium and elsewhere. It can also provide "ground truth" when suitably refined for stereo matching algorithms as well as provide visual cues as to why these matching algorithms sometimes fail to mitigate this in the future. The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 218814 "PRoVisG".

Statistical survey of widely spread out solar electron events observed with STEREO and ACE with special attention to anisotropies

NASA Astrophysics Data System (ADS)

Dresing, N.; Gómez-Herrero, R.; Heber, B.; Klassen, A.; Malandraki, O.; Dröge, W.; Kartavykh, Y.

2014-07-01

Context. In February 2011, the two STEREO spacecrafts reached a separation of 180 degrees in longitude, offering a complete view of the Sun for the first time ever. When the full Sun surface is visible, source active regions of solar energetic particle (SEP) events can be identified unambiguously. STEREO, in combination with near-Earth observatories such as ACE or SOHO, provides three well separated viewpoints, which build an unprecedented platform from which to investigate the longitudinal variations of SEP events. Aims: We show an ensemble of SEP events that were observed between 2009 and mid-2013 by at least two spacecrafts and show a remarkably wide particle spread in longitude (wide-spread events). The main selection criterion for these events was a longitudinal separation of at least 80 degrees between active region and spacecraft magnetic footpoint for the widest separated spacecraft. We investigate the events statistically in terms of peak intensities, onset delays, and rise times, and determine the spread of the longitudinal events, which is the range filled by SEPs during the events. Energetic electron anisotropies are investigated to distinguish the source and transport mechanisms that lead to the observed wide particle spreads. Methods: According to the anisotropy distributions, we divided the events into three classes depending on different source and transport scenarios. One potential mechanism for wide-spread events is efficient perpendicular transport in the interplanetary medium that competes with another scenario, which is a wide particle spread that occurs close to the Sun. In the latter case, the observations at 1 AU during the early phase of the events are expected to show significant anisotropies because of the wide injection range at the Sun and particle-focusing during the outward propagation, while in the first case only low anisotropies are anticipated. Results: We find events for both of these scenarios in our sample that match the expected observations and even different events that do not agree with the scenarios. We conclude that probably both an extended source region at the Sun and perpendicular transport in the interplanetary medium are involved for most of these wide-spread events. Appendix A is available in electronic form at http://www.aanda.org

Three-Dimensional Intercalated Porous Graphene on Si(111)

NASA Astrophysics Data System (ADS)

Pham, Trung T.; Sporken, Robert

2018-02-01

Three-dimensional intercalated porous graphene has been formed on Si(111) by electron beam evaporation under appropriate conditions and its structural and electronic properties investigated in detail by reflection high-energy electron diffraction, x-ray photoemission spectroscopy, Raman spectroscopy, high-resolution scanning electron microscopy, atomic force microscopy, and scanning tunneling microscopy. The results show that the crystalline quality of the porous graphene depended not only on the substrate temperature but also on the SiC layer thickness during carbon atom deposition.

Scanning Transmission Electron Microscopy | Materials Science | NREL

Science.gov Websites

mode by collecting the EDS and EELS signals point-by-point as one scans the electron probe across the . Examples of Scanning Transmission Electron Microscopy Capabilities Z-contrast image microphoto taken by

Low-cost, portable, robust and high-resolution single-camera stereo-DIC system and its application in high-temperature deformation measurements

NASA Astrophysics Data System (ADS)

Chi, Yuxi; Yu, Liping; Pan, Bing

2018-05-01

A low-cost, portable, robust and high-resolution single-camera stereo-digital image correlation (stereo-DIC) system for accurate surface three-dimensional (3D) shape and deformation measurements is described. This system adopts a single consumer-grade high-resolution digital Single Lens Reflex (SLR) camera and a four-mirror adaptor, rather than two synchronized industrial digital cameras, for stereo image acquisition. In addition, monochromatic blue light illumination and coupled bandpass filter imaging are integrated to ensure the robustness of the system against ambient light variations. In contrast to conventional binocular stereo-DIC systems, the developed pseudo-stereo-DIC system offers the advantages of low cost, portability, robustness against ambient light variations, and high resolution. The accuracy and precision of the developed single SLR camera-based stereo-DIC system were validated by measuring the 3D shape of a stationary sphere along with in-plane and out-of-plane displacements of a translated planar plate. Application of the established system to thermal deformation measurement of an alumina ceramic plate and a stainless-steel plate subjected to radiation heating was also demonstrated.

Diagnostic electron microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dickersin, G.R.

1988-01-01

In this book the author presents a comprehensive reference text on diagnostic electron microscopy. Throughout the book he illustrates how ultrastructural identification can be helpful for the recognition of cell type and the identification of mechanisms of pathogenesis in various diseases. In addition to electron microscopy photographs, there are also numerous light microscopy photographs for comparison. This text presents the classification of neoplasms in the order and arrangement most familiar to the pathologist. Contents: Introduction; Diagram of a Normal Cell; Normal Cell Function; Embryology; Neoplasms; Infectious Agents; Metabolic Diseases; Renal Diseases; Skeletal Muscle and Peripheral Nerve Diseases; Index.

Stereo Science Update

NASA Image and Video Library

2009-04-13

Michael Kaiser, project scientist, Solar Terrestrial Relations Observatory (STEREO) at Goddard Space Flight Center, left, makes a comment during a Science Update on the STEREO mission at NASA Headquarters in Washington, Tuesday, April 14, 2009, as Angelo Vourlidas, project scientist, Sun Earth Connection Coronal and Heliospheric Investigation, at the Naval Research Laboratory, second from left, Toni Galvin, principal investigator, Plasma and Superthermal Ion Composition instrument at the University of New Hampshire and Madhulika Guhathakurta, STEREO program scientist, right, look on. Photo Credit: (NASA/Paul E. Alers)

Stereo Science Update

NASA Image and Video Library

2009-04-13

Angelo Vourlidas, project scientist, Sun Earth Connection Coronal and Heliospheric Investigation, at the Naval Research Laboratory, second from left, makes a comment during a Science Update on the STEREO mission at NASA Headquarters in Washington, Tuesday, April 14, 2009, as Michael Kaiser, project scientist, Solar Terrestrial Relations Observatory (STEREO) at Goddard Space Flight Center, left, Toni Galvin, principal investigator, Plasma and Superthermal Ion Composition instrument at the University of New Hampshire and Madhulika Guhathakurta, STEREO program scientist, right, look on. Photo Credit: (NASA/Paul E. Alers)

Stereo vision and strabismus

PubMed Central

Read, J C A

2015-01-01

Binocular stereopsis, or stereo vision, is the ability to derive information about how far away objects are, based solely on the relative positions of the object in the two eyes. It depends on both sensory and motor abilities. In this review, I briefly outline some of the neuronal mechanisms supporting stereo vision, and discuss how these are disrupted in strabismus. I explain, in some detail, current methods of assessing stereo vision and their pros and cons. Finally, I review the evidence supporting the clinical importance of such measurements. PMID:25475234

KSC-06pd1150

NASA Image and Video Library

2006-06-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., technicians check the STEREO spacecraft "B" as it is lifted off a tilt table. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket on July 22. Photo credit: NASA/George Shelton

KSC-06pd1148

NASA Image and Video Library

2006-06-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the STEREO spacecraft "B" is being moved to a another stand nearby for testing. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket on July 22. Photo credit: NASA/George Shelton

KSC-06pd1152

NASA Image and Video Library

2006-06-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., technicians check the STEREO spacecraft "B" as it is lowered toward a stand on the floor. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket on July 22. Photo credit: NASA/George Shelton

Structural behavior of Tl-exchanged natrolite at high pressure depending on the composition of pressure-transmitting medium

NASA Astrophysics Data System (ADS)

Seryotkin, Yu. V.; Bakakin, V. V.; Likhacheva, A. Yu.; Dementiev, S. N.; Rashchenko, S. V.

2017-10-01

The structural evolution of Tl-exchanged natrolite with idealized formula Tl2[Al2Si3O10]·2H2O, compressed in penetrating (water:ethanol 1:1) and non-penetrating (paraffin) media, was studied up to 4 GPa. The presence of Tl+ with non-bonded electron lone pairs, which can be either stereo-chemically active or passive, determines distinctive features of the high-pressure behavior of the Tl-form. The effective volume of assemblages Tl+(O,H2O) n depends on the E-pairs activity: single-sided coordination correlates with smaller volumes. At ambient conditions, there are two types of Tl positions, only one of them having a nearly single-sided coordination as a characteristic of stereo-activity of the Tl+ E pair. Upon the compression in paraffin, a phase transition occurs: a 5% volume contraction of flexible natrolite framework is accompanied by the conversion of all the Tl+ cations into stereo-chemically active state with a single-sided coordination. This effect requires the reconstruction of all the extra-framework subsystems with the inversion of the cation and H2O positions. The compression in water-containing medium leads to the increase of H2O content up to three molecules pfu through the filling of partly vacant positions. This hinders a single-sided coordination of Tl ions and preserves the configuration of their ion-molecular subsystem. It is likely that the extra-framework subsystem is responsible for the super-structure modulation.

Effect of short-term exposure to stereoscopic three-dimensional flight displays on real-world depth perception

NASA Technical Reports Server (NTRS)

Busquets, Anthony M.; Parrish, Russell V.; Williams, Steven P.

1991-01-01

High-fidelity color pictorial displays that incorporate depth cues in the display elements are currently available. Depth cuing applied to advanced head-down flight display concepts potentially enhances the pilot's situational awareness and improves task performance. Depth cues provided by stereopsis exhibit constraints that must be fully understood so depth cuing enhancements can be adequately realized and exploited. A fundamental issue (the goal of this investigation) is whether the use of head-down stereoscopic displays in flight applications degrade the real-world depth perception of pilots using such displays. Stereoacuity tests are used in this study as the measure of interest. Eight pilots flew repeated simulated landing approaches using both nonstereo and stereo 3-D head-down pathway-in-the-sky displays. At this decision height of each approach (where the pilot changes to an out-the-window view to obtain real-world visual references) the pilots changed to a stereoacuity test that used real objects. Statistical analysis of stereoacuity measures (data for a control condition of no exposure to any electronic flight display compared with data for changes from nonstereo and from stereo displays) reveals no significant differences for any of the conditions. Therefore, changing from short-term exposure to a head-down stereo display has no more effect on real-world relative depth perception than does changing from a nonstereo display. However, depth perception effects based on sized and distance judgements and on long-term exposure remain issues to be investigated.

HALE STAIN FOR SIALIC ACID-CONTAINING MUCINS. ADAPTATION TO ELECTRON MICROSCOPY.

PubMed

GASIC, G; BERWICK, L

1963-10-01

The feasibility of using the Hale stain to identify cellular sialic acid-containing mucins by electron microscopy was investigated. Three kinds of mouse ascites tumor cells were fixed in neutral buffered formalin, exposed to fresh colloidal ferric oxide, treated with potassium ferrocyanide, imbedded in Selectron, and sectioned for electron microscopy. Additional staining with uranyl acetate and potassium permanganate was done after sectioning in order to increase contrast. Those cells known to be coated with sialomucin showed deposits of electron-opaque ferric ferrocyanide crystals in the areas where sialomucin concentrations were expected. When these cells were treated with neuraminidase beforehand, these deposits did not appear. It was concluded that, with the precautions and modifications described, the Hale stain can be successfully combined with electron microscopy to identify sialomucin.

Perspectives on in situ electron microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zheng, Haimei; Zhu, Yimei

In situ transmission electron microscopy (TEM) with the ability to reveal materials dynamic processes with high spatial and temporal resolution has attracted significant interest. The recent advances in in situ methods, including liquid and gas sample environment, pump-probe ultrafast microscopy, nanomechanics and ferroelectric domain switching the aberration corrected electron optics as well as fast electron detector has opened new opportunities to extend the impact of in situ TEM in broad areas of research ranging from materials science to chemistry, physics and biology. Here in this paper, we highlight the development of liquid environment electron microscopy and its applications in themore » study of colloidal nanoparticle growth, electrochemical processes and others; in situ study of topological vortices in ferroelectric and ferromagnetic materials. At the end, perspectives of future in situ TEM are provided.« less

Perspectives on in situ electron microscopy

DOE PAGES

Zheng, Haimei; Zhu, Yimei

2017-03-29

In situ transmission electron microscopy (TEM) with the ability to reveal materials dynamic processes with high spatial and temporal resolution has attracted significant interest. The recent advances in in situ methods, including liquid and gas sample environment, pump-probe ultrafast microscopy, nanomechanics and ferroelectric domain switching the aberration corrected electron optics as well as fast electron detector has opened new opportunities to extend the impact of in situ TEM in broad areas of research ranging from materials science to chemistry, physics and biology. Here in this paper, we highlight the development of liquid environment electron microscopy and its applications in themore » study of colloidal nanoparticle growth, electrochemical processes and others; in situ study of topological vortices in ferroelectric and ferromagnetic materials. At the end, perspectives of future in situ TEM are provided.« less

Cryo-Scanning Electron Microscopy (SEM) and Scanning Transmission Electron Microscopy (STEM)-in-SEM for Bio- and Organo-Mineral Interface Characterization in the Environment.

PubMed

Wille, Guillaume; Hellal, Jennifer; Ollivier, Patrick; Richard, Annie; Burel, Agnes; Jolly, Louis; Crampon, Marc; Michel, Caroline

2017-12-01

Understanding biofilm interactions with surrounding substratum and pollutants/particles can benefit from the application of existing microscopy tools. Using the example of biofilm interactions with zero-valent iron nanoparticles (nZVI), this study aims to apply various approaches in biofilm preparation and labeling for fluorescent or electron microscopy and energy dispersive X-ray spectrometry (EDS) microanalysis for accurate observations. According to the targeted microscopy method, biofilms were sampled as flocs or attached biofilm, submitted to labeling using 4',6-diamidino-2-phenylindol, lectins PNA and ConA coupled to fluorescent dye or gold nanoparticles, and prepared for observation (fixation, cross-section, freezing, ultramicrotomy). Fluorescent microscopy revealed that nZVI were embedded in the biofilm structure as aggregates but the resolution was insufficient to observe individual nZVI. Cryo-scanning electron microscopy (SEM) observations showed nZVI aggregates close to bacteria, but it was not possible to confirm direct interactions between nZVI and cell membranes. Scanning transmission electron microscopy in the SEM (STEM-in-SEM) showed that nZVI aggregates could enter the biofilm to a depth of 7-11 µm. Bacteria were surrounded by a ring of extracellular polymeric substances (EPS) preventing direct nZVI/membrane interactions. STEM/EDS mapping revealed a co-localization of nZVI aggregates with lectins suggesting a potential role of EPS in nZVI embedding. Thus, the combination of divergent microscopy approaches is a good approach to better understand and characterize biofilm/metal interactions.

On-screen-display (OSD) menu detection for proper stereo content reproduction for 3D TV

NASA Astrophysics Data System (ADS)

Tolstaya, Ekaterina V.; Bucha, Victor V.; Rychagov, Michael N.

2011-03-01

Modern consumer 3D TV sets are able to show video content in two different modes: 2D and 3D. In 3D mode, stereo pair comes from external device such as Blue-ray player, satellite receivers etc. The stereo pair is split into left and right images that are shown one after another. The viewer sees different image for left and right eyes using shutter-glasses properly synchronized with a 3DTV. Besides, some devices that provide TV with a stereo content are able to display some additional information by imposing an overlay picture on video content, an On-Screen-Display (OSD) menu. Some OSDs are not always 3D compatible and lead to incorrect 3D reproduction. In this case, TV set must recognize the type of OSD, whether it is 3D compatible, and visualize it correctly by either switching off stereo mode, or continue demonstration of stereo content. We propose a new stable method for detection of 3D incompatible OSD menus on stereo content. Conventional OSD is a rectangular area with letters and pictograms. OSD menu can be of different transparency levels and colors. To be 3D compatible, an OSD is overlaid separately on both images of a stereo pair. The main problem in detecting OSD is to distinguish whether the color difference is due to OSD presence, or due to stereo parallax. We applied special techniques to find reliable image difference and additionally used a cue that usually OSD has very implicit geometrical features: straight parallel lines. The developed algorithm was tested on our video sequences database, with several types of OSD with different colors and transparency levels overlaid upon video content. Detection quality exceeded 99% of true answers.

Augmented reality glass-free three-dimensional display with the stereo camera

NASA Astrophysics Data System (ADS)

Pang, Bo; Sang, Xinzhu; Chen, Duo; Xing, Shujun; Yu, Xunbo; Yan, Binbin; Wang, Kuiru; Yu, Chongxiu

2017-10-01

An improved method for Augmented Reality (AR) glass-free three-dimensional (3D) display based on stereo camera used for presenting parallax contents from different angle with lenticular lens array is proposed. Compared with the previous implementation method of AR techniques based on two-dimensional (2D) panel display with only one viewpoint, the proposed method can realize glass-free 3D display of virtual objects and real scene with 32 virtual viewpoints. Accordingly, viewers can get abundant 3D stereo information from different viewing angles based on binocular parallax. Experimental results show that this improved method based on stereo camera can realize AR glass-free 3D display, and both of virtual objects and real scene have realistic and obvious stereo performance.

SPECTRA. September 2011

DTIC Science & Technology

2011-09-01

Spots 2000th Comet 14 LASCO: 13,587 CMEs and Counting 15 Viewing the Sun in 3-D with STEREO 18 NRL Launches Nanosatellite Experimental Platforms...specifically count the most abundant particles in the solar wind — electrons, protons, and helium ions — and measure their proper- ties. The...and Counting NRL FEATURES S O L A R P H Y S IC S Total mass injection in the solar wind by CMEs over the last 14 years as observed by the LASCO

Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells.

PubMed

Koh, Ai Leen; Shachaf, Catherine M; Elchuri, Sailaja; Nolan, Garry P; Sinclair, Robert

2008-12-01

We demonstrate the use of electron microscopy as a powerful characterization tool to identify and locate antibody-conjugated composite organic-inorganic nanoparticle (COINs) surface enhanced Raman scattering (SERS) nanoparticles on cells. U937 leukemia cells labeled with antibody CD54-conjugated COINs were characterized in their native, hydrated state using wet scanning electron microscopy (SEM) and in their dehydrated state using high-resolution SEM. In both cases, the backscattered electron (BSE) detector was used to detect and identify the silver constituents in COINs due to its high sensitivity to atomic number variations within a specimen. The imaging and analytical capabilities in the SEM were further complemented by higher resolution transmission electron microscopy (TEM) images and scanning Auger electron spectroscopy (AES) data to give reliable and high-resolution information about nanoparticles and their binding to cell surface antigens.

Three-dimensional electron microscopy simulation with the CASINO Monte Carlo software.

PubMed

Demers, Hendrix; Poirier-Demers, Nicolas; Couture, Alexandre Réal; Joly, Dany; Guilmain, Marc; de Jonge, Niels; Drouin, Dominique

2011-01-01

Monte Carlo softwares are widely used to understand the capabilities of electron microscopes. To study more realistic applications with complex samples, 3D Monte Carlo softwares are needed. In this article, the development of the 3D version of CASINO is presented. The software feature a graphical user interface, an efficient (in relation to simulation time and memory use) 3D simulation model, accurate physic models for electron microscopy applications, and it is available freely to the scientific community at this website: www.gel.usherbrooke.ca/casino/index.html. It can be used to model backscattered, secondary, and transmitted electron signals as well as absorbed energy. The software features like scan points and shot noise allow the simulation and study of realistic experimental conditions. This software has an improved energy range for scanning electron microscopy and scanning transmission electron microscopy applications. Copyright © 2011 Wiley Periodicals, Inc.

Three-Dimensional Electron Microscopy Simulation with the CASINO Monte Carlo Software

PubMed Central

Demers, Hendrix; Poirier-Demers, Nicolas; Couture, Alexandre Réal; Joly, Dany; Guilmain, Marc; de Jonge, Niels; Drouin, Dominique

2011-01-01

Monte Carlo softwares are widely used to understand the capabilities of electron microscopes. To study more realistic applications with complex samples, 3D Monte Carlo softwares are needed. In this paper, the development of the 3D version of CASINO is presented. The software feature a graphical user interface, an efficient (in relation to simulation time and memory use) 3D simulation model, accurate physic models for electron microscopy applications, and it is available freely to the scientific community at this website: www.gel.usherbrooke.ca/casino/index.html. It can be used to model backscattered, secondary, and transmitted electron signals as well as absorbed energy. The software features like scan points and shot noise allow the simulation and study of realistic experimental conditions. This software has an improved energy range for scanning electron microscopy and scanning transmission electron microscopy applications. PMID:21769885

Stereographic observations from geosynchronous satellites - An important new tool for the atmospheric sciences

NASA Technical Reports Server (NTRS)

Hasler, A. F.

1981-01-01

Observations of cloud geometry using scan-synchronized stereo geostationary satellites having images with horizontal spatial resolution of approximately 0.5 km, and temporal resolution of up to 3 min are presented. The stereo does not require a cloud with known emissivity to be in equilibrium with an atmosphere with a known vertical temperature profile. It is shown that absolute accuracies of about 0.5 km are possible. Qualitative and quantitative representations of atmospheric dynamics were shown by remapping, display, and stereo image analysis on an interactive computer/imaging system. Applications of stereo observations include: (1) cloud top height contours of severe thunderstorms and hurricanes, (2) cloud top and base height estimates for cloud-wind height assignment, (3) cloud growth measurements for severe thunderstorm over-shooting towers, (4) atmospheric temperature from stereo heights and infrared cloud top temperatures, and (5) cloud emissivity estimation. Recommendations are given for future improvements in stereo observations, including a third GOES satellite, operational scan synchronization of all GOES satellites and better resolution sensors.

Chemical mapping and quantification at the atomic scale by scanning transmission electron microscopy.

PubMed

Chu, Ming-Wen; Chen, Cheng Hsuan

2013-06-25

With innovative modern material-growth methods, a broad spectrum of fascinating materials with reduced dimensions-ranging from single-atom catalysts, nanoplasmonic and nanophotonic materials to two-dimensional heterostructural interfaces-is continually emerging and extending the new frontiers of materials research. A persistent central challenge in this grand scientific context has been the detailed characterization of the individual objects in these materials with the highest spatial resolution, a problem prompting the need for experimental techniques that integrate both microscopic and spectroscopic capabilities. To date, several representative microscopy-spectroscopy combinations have become available, such as scanning tunneling microscopy, tip-enhanced scanning optical microscopy, atom probe tomography, scanning transmission X-ray microscopy, and scanning transmission electron microscopy (STEM). Among these tools, STEM boasts unique chemical and electronic sensitivity at unparalleled resolution. In this Perspective, we elucidate the advances in STEM and chemical mapping applications at the atomic scale by energy-dispersive X-ray spectroscopy and electron energy loss spectroscopy with a focus on the ultimate challenge of chemical quantification with atomic accuracy.

Neurite density from magnetic resonance diffusion measurements at ultrahigh field: Comparison with light microscopy and electron microscopy

PubMed Central

Jespersen, Sune N.; Bjarkam, Carsten R.; Nyengaard, Jens R.; Chakravarty, M. Mallar; Hansen, Brian; Vosegaard, Thomas; Østergaard, Leif; Yablonskiy, Dmitriy; Nielsen, Niels Chr.; Vestergaard-Poulsen, Peter

2010-01-01

Due to its unique sensitivity to tissue microstructure, diffusion-weighted magnetic resonance imaging (MRI) has found many applications in clinical and fundamental science. With few exceptions, a more precise correspondence between physiological or biophysical properties and the obtained diffusion parameters remain uncertain due to lack of specificity. In this work, we address this problem by comparing diffusion parameters of a recently introduced model for water diffusion in brain matter to light microscopy and quantitative electron microscopy. Specifically, we compare diffusion model predictions of neurite density in rats to optical myelin staining intensity and stereological estimation of neurite volume fraction using electron microscopy. We find that the diffusion model describes data better and that its parameters show stronger correlation with optical and electron microscopy, and thus reflect myelinated neurite density better than the more frequently used diffusion tensor imaging (DTI) and cumulant expansion methods. Furthermore, the estimated neurite orientations capture dendritic architecture more faithfully than DTI diffusion ellipsoids. PMID:19732836

Application of high-angle annular dark field scanning transmission electron microscopy, scanning transmission electron microscopy-energy dispersive X-ray spectrometry, and energy-filtered transmission electron microscopy to the characterization of nanoparticles in the environment.

PubMed

Utsunomiya, Satoshi; Ewing, Rodney C

2003-02-15

A major challenge to the development of a fundamental understanding of transport and retardation mechanisms of trace metal contaminants (<10 ppm) is their identification and characterization at the nanoscale. Atomic-scale techniques, such as conventional transmission electron microscopy, although powerful, are limited by the extremely small amounts of material that are examined. However, recent advances in electron microscopy provide a number of new analytical techniques that expand its application in environmental studies, particularly those concerning heavy metals on airborne particulates or water-borne colloids. High-angle annular dark field scanning transmission electron microscopy (HAADF-STEM), STEM-energy-dispersive X-ray spectrometry (EDX), and energy-filtered TEM (EFTEM) can be effectively used to identify and characterize nanoparticles. The image contrast in HAADF-STEM is strongly correlated to the atomic mass: heavier elements contribute to brighter contrast. Gold nanocrystals in pyrite and uranium nanocrystals in atmospheric aerosols have been identified by HAADF-STEM and STEM-EDX mapping and subsequently characterized by high-resolution TEM (HRTEM). EFTEM was used to identify U and Fe nanocrystals embedded in an aluminosilicate. A rare, As-bearing nanophase, westerveldite (FeAs), was identified by STEM-EDX and HRTEM. The combined use of these techniques greatly expands the effective application of electron microscopy in environmental studies, especially when applied to metals of very low concentrations. This paper describes examples of how these electron microbeam techniques can be used in combination to characterize a low concentration of heavy metals (a few ppm) on nanoscale particles.

Consecutive light microscopy, scanning-transmission electron microscopy and transmission electron microscopy of traumatic human brain oedema and ischaemic brain damage.

PubMed

Castejon, O J; Castejon, H V; Diaz, M; Castellano, A

2001-10-01

Cortical biopsies of 11 patients with traumatic brain oedema were consecutively studied by light microscopy (LM) using thick plastic sections, scanning-transmission electron microscopy ((S)TEM) using semithin plastic sections and transmission electron microscopy (TEM) using ultrathin sections. Samples were glutaraldehyde-osmium fixed and embedded in Araldite or Epon. Thick sections were stained with toluidine-blue for light microscopy. Semithin sections were examined unstained and uncoated for (S)TEM. Ultrathin sections were stained with uranyl and lead. Perivascular haemorrhages and perivascular extravasation of proteinaceous oedema fluid were observed in both moderate and severe oedema. Ischaemic pyramidal and non-pyramidal nerve cells appeared shrunken, electron dense and with enlargement of intracytoplasmic membrane compartment. Notably swollen astrocytes were observed in all samples examined. Glycogen-rich and glycogen-depleted astrocytes were identified in anoxic-ischaemic regions. Dark and hydropic satellite, interfascicular and perivascular oligodendrocytes were also found. The status spongiosus of severely oedematous brain parenchyma observed by LM and (S)TEM was correlated with the enlarged extracellular space and disrupted neuropil observed by TEM. The (S)TEM is recommended as a suitable technique for studying pathological processes in the central nervous system and as an informative adjunct to LM and TEM.

Contributed review: Review of integrated correlative light and electron microscopy.

PubMed

Timmermans, F J; Otto, C

2015-01-01

New developments in the field of microscopy enable to acquire increasing amounts of information from large sample areas and at an increased resolution. Depending on the nature of the technique, the information may reveal morphological, structural, chemical, and still other sample characteristics. In research fields, such as cell biology and materials science, there is an increasing demand to correlate these individual levels of information and in this way to obtain a better understanding of sample preparation and specific sample properties. To address this need, integrated systems were developed that combine nanometer resolution electron microscopes with optical microscopes, which produce chemically or label specific information through spectroscopy. The complementary information from electron microscopy and light microscopy presents an opportunity to investigate a broad range of sample properties in a correlated fashion. An important part of correlating the differences in information lies in bridging the different resolution and image contrast features. The trend to analyse samples using multiple correlated microscopes has resulted in a new research field. Current research is focused, for instance, on (a) the investigation of samples with nanometer scale distribution of inorganic and organic materials, (b) live cell analysis combined with electron microscopy, and (c) in situ spectroscopic and electron microscopy analysis of catalytic materials, but more areas will benefit from integrated correlative microscopy.

Carbon contamination in scanning transmission electron microscopy and its impact on phase-plate applications.

PubMed

Hettler, Simon; Dries, Manuel; Hermann, Peter; Obermair, Martin; Gerthsen, Dagmar; Malac, Marek

2017-05-01

We analyze electron-beam induced carbon contamination in a transmission electron microscope. The study is performed on thin films potentially suitable as phase plates for phase-contrast transmission electron microscopy. Electron energy-loss spectroscopy and phase-plate imaging is utilized to analyze the contamination. The deposited contamination layer is identified as a graphitic carbon layer which is not prone to electrostatic charging whereas a non-conductive underlying substrate charges. Several methods that inhibit contamination are evaluated and the impact of carbon contamination on phase-plate imaging is discussed. The findings are in general interesting for scanning transmission electron microscopy applications. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

Evaluation of anterior lenticonus in alport syndrome using tracey wavefront aberrometry and transmission electron microscopy.

PubMed

Kim, Kwan Soo; Kim, Mo Sae; Kim, Joon Mo; Choi, Chul Young

2010-01-01

To evaluate the efficacy of Tracey wavefront aberrometry (Tracey Technologies, Houston, TX) and transmission electron microscopy for the detection of anterior lenticonus in Alport syndrome. Tracey wavefront aberrometry was used to treat a patient with bilateral anterior lenticonus who had a history of Alport syndrome. For transmission electron microscopic examination, anterior lens capsules were obtained during clear lens phacoemulsification and intraocular lens implantation. Spherical aberrations were the predominant higher-order aberrations in the internal optics of both eyes. The Tracey wavefront aberrometer showed that most of the irregular astigmatism originated from the lenticular portion. Transmission electron microscopy of the specimens showed anterior lens capsules with decreased thickness and multiple dehiscences. Tracey wavefront aberrometry and transmission electron microscopy are effective tools for evaluation of anterior lenticonus in Alport syndrome. Copyright 2010, SLACK Incorporated.

On the state of crystallography at the dawn of the electron microscopy revolution.

PubMed

Higgins, Matthew K; Lea, Susan M

2017-10-01

While protein crystallography has, for many years, been the most used method for structural analysis of macromolecular complexes, remarkable recent advances in high-resolution electron cryo-microscopy led to suggestions that 'the revolution will not be crystallised'. Here we highlight the current success rate, speed and ease of modern crystallographic structure determination and some recent triumphs of both 'classical' crystallography and the use of X-ray free electron lasers. We also outline fundamental differences between structure determination using X-ray crystallography and electron microscopy. We suggest that crystallography will continue to co-exist with electron microscopy as part of an integrated array of methods, allowing structural biologists to focus on fundamental biological questions rather than being constrained by the methods available. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

Fabrication of ZnS nanoparticle chains on a protein template

PubMed Central

Hulleman, J.; Kim, S. M.; Tumkur, T.; Rochet, J.-C.; Stach, E.; Stanciu, L.

2011-01-01

In the present study, we have exploited the properties of a fibrillar protein for the template synthesis of zinc sulfide (ZnS) nanoparticle chains. The diameter of the ZnS nanoparticle chains was tuned in range of ~30 to ~165 nm by varying the process variables. The nanoparticle chains were characterized by field emission scanning electron microscopy, UV–Visible spectroscopy, transmission electron microscopy, electron energy loss spectroscopy, and high-resolution transmission electron microscopy. The effect of incubation temperature on the morphology of the nanoparticle chains was also studied. PMID:21804765

Using terrestrial stereo photography to interpret changes in tree quality characteristics

Treesearch

David L. Sonderman

1980-01-01

A technique is described for using stereo photography to evaluate tree quality changes over time. Stereo pairs were taken four times over an 18-year period. All four faces of the selected trees were photographed. Individual defect changes are shown for young upland white oak trees.

KSC-06pd1149

NASA Image and Video Library

2006-06-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., a technician works a guideline to the overhead crane as the STEREO spacecraft "B" is being moved to a stand nearby for testing. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket on July 22. Photo credit: NASA/George Shelton

KSC-06pd1553

NASA Image and Video Library

2006-07-13

KENNEDY SPACE CENTER, FLA. - Inside the mobile service tower on Launch Pad 17-B on Cape Canaveral Air Force Station, workers help guide the Boeing Delta II second stage for the STEREO launch onto the first stage for mating. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off in August 2006. Photo credit: NASA/George Shelton

KSC-06pd1151

NASA Image and Video Library

2006-06-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., technicians check the STEREO spacecraft "B" as it moves away from a tilt table (at right). The spacecraft will be placed on another stand nearby. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket on July 22. Photo credit: NASA/George Shelton

Correlation of live-cell imaging with volume scanning electron microscopy.

PubMed

Lucas, Miriam S; Günthert, Maja; Bittermann, Anne Greet; de Marco, Alex; Wepf, Roger

2017-01-01

Live-cell imaging is one of the most widely applied methods in live science. Here we describe two setups for live-cell imaging, which can easily be combined with volume SEM for correlative studies. The first procedure applies cell culture dishes with a gridded glass support, which can be used for any light microscopy modality. The second approach is a flow-chamber setup based on Ibidi μ-slides. Both live-cell imaging strategies can be followed up with serial blockface- or focused ion beam-scanning electron microscopy. Two types of resin embedding after heavy metal staining and dehydration are presented making best use of the particular advantages of each imaging modality: classical en-bloc embedding and thin-layer plastification. The latter can be used only for focused ion beam-scanning electron microscopy, but is advantageous for studying cell-interactions with specific substrates, or when the substrate cannot be removed. En-bloc embedding has diverse applications and can be applied for both described volume scanning electron microscopy techniques. Finally, strategies for relocating the cell of interest are discussed for both embedding approaches and in respect to the applied light and scanning electron microscopy methods. Copyright © 2017 Elsevier Inc. All rights reserved.

Possibilities and limitations of current stereo-endoscopy.

PubMed

Mueller-Richter, U D A; Limberger, A; Weber, P; Ruprecht, K W; Spitzer, W; Schilling, M

2004-06-01

Stereo-endoscopy has become a commonly used technology. In many comparative studies striking advantages of stereo-endoscopy over two-dimensional presentation could not be proven. To show the potential and fields for further improvement of this technology is the aim of this article. The physiological basis of three-dimensional vision limitations of current stereo-endoscopes is discussed and fields for further research are indicated. New developments in spatial picture acquisition and spatial picture presentation are discussed. Current limitations of stereo-endoscopy that prevent a better ranking in comparative studies with two-dimensional presentation are mainly based on insufficient picture acquisition. Devices for three-dimensional picture presentation are at a more advanced developmental stage than devices for three-dimensional picture acquisition. Further research should emphasize the development of new devices for three-dimensional picture acquisition.

Infrared stereo calibration for unmanned ground vehicle navigation

NASA Astrophysics Data System (ADS)

Harguess, Josh; Strange, Shawn

2014-06-01

The problem of calibrating two color cameras as a stereo pair has been heavily researched and many off-the-shelf software packages, such as Robot Operating System and OpenCV, include calibration routines that work in most cases. However, the problem of calibrating two infrared (IR) cameras for the purposes of sensor fusion and point could generation is relatively new and many challenges exist. We present a comparison of color camera and IR camera stereo calibration using data from an unmanned ground vehicle. There are two main challenges in IR stereo calibration; the calibration board (material, design, etc.) and the accuracy of calibration pattern detection. We present our analysis of these challenges along with our IR stereo calibration methodology. Finally, we present our results both visually and analytically with computed reprojection errors.

GLD100 - Lunar topography from LROC WAC stereo

NASA Astrophysics Data System (ADS)

Scholten, F.; Oberst, J.; Robinson, M. S.

2011-10-01

The LROC WAC instrument of the LRO mission comprises substantial stereo image data from adjacent orbits. Multiple coverage of the entire surface of the Moon at a mean ground scale of 75 m/pxl has already been achieved within the first two years of the mission. We applied photogrammetric stereo processing methods for the derivation of a 100 m raster DTM (digital terrain model), called GLD100, from several tens of thousands stereo models. The GLD100 covers the lunar surface between 80° northern and southern latitude. Polar regions are excluded because of poor illumination and stereo conditions. Vertical differences of the GLD100 to altimetry data from the LRO LOLA instrument are small, the mean deviation is typically about 20 m, without systematic lateral or vertical offsets.

Comparison of the depth of an optic nerve head obtained using stereo retinal images and HRT

NASA Astrophysics Data System (ADS)

Nakagawa, Toshiaki; Hayashi, Yoshinori; Hatanaka, Yuji; Aoyama, Akira; Hara, Takeshi; Kakogawa, Masakatsu; Fujita, Hiroshi; Yamamoto, Tetsuya

2007-03-01

The analysis of the optic nerve head (ONH) in the retinal fundus is important for the early detection of glaucoma. In this study, we investigate an automatic reconstruction method for producing the 3-D structure of the ONH from a stereo retinal image pair; the depth value of the ONH measured by using this method was compared with the measurement results determined from the Heidelberg Retina Tomograph (HRT). We propose a technique to obtain the depth value from the stereo image pair, which mainly consists of four steps: (1) cutout of the ONH region from the retinal images, (2) registration of the stereo pair, (3) disparity detection, and (4) depth calculation. In order to evaluate the accuracy of this technique, the shape of the depression of an eyeball phantom that had a circular dent as generated from the stereo image pair and used to model the ONH was compared with a physically measured quantity. The measurement results obtained when the eyeball phantom was used were approximately consistent. The depth of the ONH obtained using the stereo retinal images was in accordance with the results obtained using the HRT. These results indicate that the stereo retinal images could be useful for assessing the depth of the ONH for the diagnosis of glaucoma.

Relation Between the 3D-Geometry of the Coronal Wave and Associated CME During the 26 April 2008 Event

NASA Technical Reports Server (NTRS)

Temmer, M.; Veronig, A. M.; Gopalswamy, N.; Yashiro, S.

2011-01-01

We study the kinematical characteristics and 3D geometry of a large-scale coronal wave that occurred in association with the 26 April 2008 flare-CME event. The wave was observed with the EUVI instruments aboard both STEREO spacecraft (STEREO-A and STEREO-B) with a mean speed of approx 240 km/s. The wave is more pronounced in the eastern propagation direction, and is thus, better observable in STEREO-B images. From STEREO-B observations we derive two separate initiation centers for the wave, and their locations fit with the coronal dimming regions. Assuming a simple geometry of the wave we reconstruct its 3D nature from combined STEREO-A and STEREO-B observations. We find that the wave structure is asymmetric with an inclination toward East. The associated CME has a deprojected speed of approx 750 +/- 50 km/s, and it shows a non-radial outward motion toward the East with respect to the underlying source region location. Applying the forward fitting model developed by Thernisien, Howard, and Vourlidas we derive the CME flux rope position on the solar surface to be close to the dimming regions. We conclude that the expanding flanks of the CME most likely drive and shape the coronal wave.

WASS: an open-source stereo processing pipeline for sea waves 3D reconstruction

NASA Astrophysics Data System (ADS)

Bergamasco, Filippo; Benetazzo, Alvise; Torsello, Andrea; Barbariol, Francesco; Carniel, Sandro; Sclavo, Mauro

2017-04-01

Stereo 3D reconstruction of ocean waves is gaining more and more popularity in the oceanographic community. In fact, recent advances of both computer vision algorithms and CPU processing power can now allow the study of the spatio-temporal wave fields with unprecedented accuracy, especially at small scales. Even if simple in theory, multiple details are difficult to be mastered for a practitioner so that the implementation of a 3D reconstruction pipeline is in general considered a complex task. For instance, camera calibration, reliable stereo feature matching and mean sea-plane estimation are all factors for which a well designed implementation can make the difference to obtain valuable results. For this reason, we believe that the open availability of a well-tested software package that automates the steps from stereo images to a 3D point cloud would be a valuable addition for future researches in this area. We present WASS, a completely Open-Source stereo processing pipeline for sea waves 3D reconstruction, available at http://www.dais.unive.it/wass/. Our tool completely automates the recovery of dense point clouds from stereo images by providing three main functionalities. First, WASS can automatically recover the extrinsic parameters of the stereo rig (up to scale) so that no delicate calibration has to be performed on the field. Second, WASS implements a fast 3D dense stereo reconstruction procedure so that an accurate 3D point cloud can be computed from each stereo pair. We rely on the well-consolidated OpenCV library both for the image stereo rectification and disparity map recovery. Lastly, a set of 2D and 3D filtering techniques both on the disparity map and the produced point cloud are implemented to remove the vast majority of erroneous points that can naturally arise while analyzing the optically complex nature of the water surface (examples are sun-glares, large white-capped areas, fog and water areosol, etc). Developed to be as fast as possible, WASS can process roughly four 5 MPixel stereo frames per minute (on a consumer i7 CPU) to produce a sequence of outlier-free point clouds with more than 3 million points each. Finally, it comes with an easy to use user interface and designed to be scalable on multiple parallel CPUs.

The importance of transmission electron microscopy analysis of spermatozoa: Diagnostic applications and basic research.

PubMed

Moretti, Elena; Sutera, Gaetano; Collodel, Giulia

2016-06-01

This review is aimed at discussing the role of ultrastructural studies on human spermatozoa and evaluating transmission electron microscopy as a diagnostic tool that can complete andrology protocols. It is clear that morphological sperm defects may explain decreased fertilizing potential and acquire particular value in the field of male infertility. Electron microscopy is the best method to identify systematic or monomorphic and non-systematic or polymorphic sperm defects. The systematic defects are characterized by a particular anomaly that affects the vast majority of spermatozoa in a semen sample, whereas a heterogeneous combination of head and tail defects found in variable percentages are typically non-systematic or polymorphic sperm defects. A correct diagnosis of these specific sperm alterations is important for choosing the male infertility's therapy and for deciding to turn to assisted reproduction techniques. Transmission electron microscopy (TEM) also represents a valuable method to explore the in vitro effects of different compounds (for example drugs with potential spermicidal activity) on the morphology of human spermatozoa. Finally, TEM used in combination with immunohistochemical techniques, integrates structural and functional aspects that provide a wide horizon in the understanding of sperm physiology and pathology. transmission electron microscopy: TEM; World Health Organization: WHO; light microscopy: LM; motile sperm organelle morphology examination: MSOME; intracytoplasmic morphologically selected sperm injection: IMSI; intracytoplasmic sperm injection: ICSI; dysplasia of fibrous sheath: DFS; primary ciliary dyskinesia: PCD; outer dense fibers: ODF; assisted reproduction technologies: ART; scanning electron microscopy: SEM; polyvinylpirrolidone: PVP; tert-butylhydroperoxide: TBHP.

Diffraction and microscopy with attosecond electron pulse trains

NASA Astrophysics Data System (ADS)

Morimoto, Yuya; Baum, Peter

2018-03-01

Attosecond spectroscopy1-7 can resolve electronic processes directly in time, but a movie-like space-time recording is impeded by the too long wavelength ( 100 times larger than atomic distances) or the source-sample entanglement in re-collision techniques8-11. Here we advance attosecond metrology to picometre wavelength and sub-atomic resolution by using free-space electrons instead of higher-harmonic photons1-7 or re-colliding wavepackets8-11. A beam of 70-keV electrons at 4.5-pm de Broglie wavelength is modulated by the electric field of laser cycles into a sequence of electron pulses with sub-optical-cycle duration. Time-resolved diffraction from crystalline silicon reveals a < 10-as delay of Bragg emission and demonstrates the possibility of analytic attosecond-ångström diffraction. Real-space electron microscopy visualizes with sub-light-cycle resolution how an optical wave propagates in space and time. This unification of attosecond science with electron microscopy and diffraction enables space-time imaging of light-driven processes in the entire range of sample morphologies that electron microscopy can access.

HANFORD WASTE MINERALOGY REFERENCE REPORT

DOE Office of Scientific and Technical Information (OSTI.GOV)

DISSELKAMP RS

2010-06-29

This report lists the observed mineral phases present in the Hanford tanks. This task was accomplished by performing a review of numerous reports that used experimental techniques including, but not limited to: x-ray diffraction, polarized light microscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, electron energy loss spectroscopy, and particle size distribution analyses. This report contains tables that can be used as a quick reference to identify the crystal phases observed in Hanford waste.

HANFORD WASTE MINEROLOGY REFERENCE REPORT

DOE Office of Scientific and Technical Information (OSTI.GOV)

DISSELKAMP RS

2010-06-18

This report lists the observed mineral phase phases present in the Hanford tanks. This task was accomplished by performing a review of numerous reports using experimental techniques including, but not limited to: x-ray diffraction, polarized light microscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, electron energy loss spectroscopy, and particle size distribution analyses. This report contains tables that can be used as a quick reference to identify the crystal phases present observed in Hanford waste.

Three dimensional electron microscopy and in silico tools for macromolecular structure determination

PubMed Central

Borkotoky, Subhomoi; Meena, Chetan Kumar; Khan, Mohammad Wahab; Murali, Ayaluru

2013-01-01

Recently, structural biology witnessed a major tool - electron microscopy - in solving the structures of macromolecules in addition to the conventional techniques, X-ray crystallography and nuclear magnetic resonance (NMR). Three dimensional transmission electron microscopy (3DTEM) is one of the most sophisticated techniques for structure determination of molecular machines. Known to give the 3-dimensional structures in its native form with literally no upper limit on size of the macromolecule, this tool does not need the crystallization of the protein. Combining the 3DTEM data with in silico tools, one can have better refined structure of a desired complex. In this review we are discussing about the recent advancements in three dimensional electron microscopy and tools associated with it. PMID:27092033

Tackling the Challenges of Dynamic Experiments Using Liquid-Cell Transmission Electron Microscopy.

PubMed

Parent, Lucas R; Bakalis, Evangelos; Proetto, Maria; Li, Yiwen; Park, Chiwoo; Zerbetto, Francesco; Gianneschi, Nathan C

2018-01-16

Revolutions in science and engineering frequently result from the development, and wide adoption, of a new, powerful characterization or imaging technique. Beginning with the first glass lenses and telescopes in astronomy, to the development of visual-light microscopy, staining techniques, confocal microscopy, and fluorescence super-resolution microscopy in biology, and most recently aberration-corrected, cryogenic, and ultrafast (4D) electron microscopy, X-ray microscopy, and scanning probe microscopy in nanoscience. Through these developments, our perception and understanding of the physical nature of matter at length-scales beyond ordinary perception have been fundamentally transformed. Despite this progression in microscopy, techniques for observing nanoscale chemical processes and solvated/hydrated systems are limited, as the necessary spatial and temporal resolution presents significant technical challenges. However, the standard reliance on indirect or bulk phase characterization of nanoscale samples in liquids is undergoing a shift in recent times with the realization ( Williamson et al. Nat. Mater . 2003 , 2 , 532 - 536 ) of liquid-cell (scanning) transmission electron microscopy, LC(S)TEM, where picoliters of solution are hermetically sealed between electron-transparent "windows," which can be directly imaged or videoed at the nanoscale using conventional transmission electron microscopes. This Account seeks to open a discussion on the topic of standardizing strategies for conducting imaging experiments with a view to characterizing dynamics and motion of nanoscale materials. This is a challenge that could be described by critics and proponents alike, as analogous to doing chemistry in a lightning storm; where the nature of the solution, the nanomaterial, and the dynamic behaviors are all potentially subject to artifactual influence by the very act of our observation.

Nano-fEM: protein localization using photo-activated localization microscopy and electron microscopy.

PubMed

Watanabe, Shigeki; Richards, Jackson; Hollopeter, Gunther; Hobson, Robert J; Davis, Wayne M; Jorgensen, Erik M

2012-12-03

Mapping the distribution of proteins is essential for understanding the function of proteins in a cell. Fluorescence microscopy is extensively used for protein localization, but subcellular context is often absent in fluorescence images. Immuno-electron microscopy, on the other hand, can localize proteins, but the technique is limited by a lack of compatible antibodies, poor preservation of morphology and because most antigens are not exposed to the specimen surface. Correlative approaches can acquire the fluorescence image from a whole cell first, either from immuno-fluorescence or genetically tagged proteins. The sample is then fixed and embedded for electron microscopy, and the images are correlated (1-3). However, the low-resolution fluorescence image and the lack of fiducial markers preclude the precise localization of proteins. Alternatively, fluorescence imaging can be done after preserving the specimen in plastic. In this approach, the block is sectioned, and fluorescence images and electron micrographs of the same section are correlated (4-7). However, the diffraction limit of light in the correlated image obscures the locations of individual molecules, and the fluorescence often extends beyond the boundary of the cell. Nano-resolution fluorescence electron microscopy (nano-fEM) is designed to localize proteins at nano-scale by imaging the same sections using photo-activated localization microscopy (PALM) and electron microscopy. PALM overcomes the diffraction limit by imaging individual fluorescent proteins and subsequently mapping the centroid of each fluorescent spot (8-10). We outline the nano-fEM technique in five steps. First, the sample is fixed and embedded using conditions that preserve the fluorescence of tagged proteins. Second, the resin blocks are sectioned into ultrathin segments (70-80 nm) that are mounted on a cover glass. Third, fluorescence is imaged in these sections using the Zeiss PALM microscope. Fourth, electron dense structures are imaged in these same sections using a scanning electron microscope. Fifth, the fluorescence and electron micrographs are aligned using gold particles as fiducial markers. In summary, the subcellular localization of fluorescently tagged proteins can be determined at nanometer resolution in approximately one week.

CHAMP (Camera, Handlens, and Microscope Probe)

NASA Technical Reports Server (NTRS)

Mungas, Greg S.; Boynton, John E.; Balzer, Mark A.; Beegle, Luther; Sobel, Harold R.; Fisher, Ted; Klein, Dan; Deans, Matthew; Lee, Pascal; Sepulveda, Cesar A.

2005-01-01

CHAMP (Camera, Handlens And Microscope Probe)is a novel field microscope capable of color imaging with continuously variable spatial resolution from infinity imaging down to diffraction-limited microscopy (3 micron/pixel). As a robotic arm-mounted imager, CHAMP supports stereo imaging with variable baselines, can continuously image targets at an increasing magnification during an arm approach, can provide precision rangefinding estimates to targets, and can accommodate microscopic imaging of rough surfaces through a image filtering process called z-stacking. CHAMP was originally developed through the Mars Instrument Development Program (MIDP) in support of robotic field investigations, but may also find application in new areas such as robotic in-orbit servicing and maintenance operations associated with spacecraft and human operations. We overview CHAMP'S instrument performance and basic design considerations below.

Field Geology/Processes

NASA Technical Reports Server (NTRS)

Allen, Carlton; Jakes, Petr; Jaumann, Ralf; Marshall, John; Moses, Stewart; Ryder, Graham; Saunders, Stephen; Singer, Robert

1996-01-01

The field geology/process group examined the basic operations of a terrestrial field geologist and the manner in which these operations could be transferred to a planetary lander. Four basic requirements for robotic field geology were determined: geologic content; surface vision; mobility; and manipulation. Geologic content requires a combination of orbital and descent imaging. Surface vision requirements include range, resolution, stereo, and multispectral imaging. The minimum mobility for useful field geology depends on the scale of orbital imagery. Manipulation requirements include exposing unweathered surfaces, screening samples, and bringing samples in contact with analytical instruments. To support these requirements, several advanced capabilities for future development are recommended. Capabilities include near-infrared reflectance spectroscopy, hyper-spectral imaging, multispectral microscopy, artificial intelligence in support of imaging, x ray diffraction, x ray fluorescence, and rock chipping.

Exploring the relative reactivities of the hydroxyl groups of monosaccharides by molecular modeling and molecular mechanics

NASA Astrophysics Data System (ADS)

Box, V. G. S.; Evans-Lora, T.

2000-01-01

The molecular modeling program STR3DI.EXE, and its molecular mechanics module, QVBMM, were used to simulate, and evaluate, the stereo-electronic effects in the mono-alkoxides of the 4,6- O-ethylideneglycopyranosides of allose, mannose, galactose and glucose. This study has confirmed the ability of these molecular modeling tools to predict the regiochemistry and reactivity of these sugar derivatives, and holds considerable implications for unraveling the chemistry of the rare monosaccharides.

Protecting Group and Solvent Control of Stereo- and Chemoselectivity in Glucal 3-Carbamate Amidoglycosylation

PubMed Central

Gupta, Ritu; Sogi, Kimberly M.; Bernard, Sarah E.; Decatur, John D.; Rojas, Christian M.

2009-01-01

In the Rh2(OAc)4-catalyzed amidoglycosylation of glucal 3-carbamates, anomeric stereoselectivity and the extent of competing C3–H oxidation depend on the 4O and 6O protecting groups. Acyclic protection permits high α-anomer selectivity with further improvement in less polar solvents, while electron-withdrawing protecting groups limit C3-oxidized byproducts. Stereocontrol and bifurcation between alkene insertion and C3–H oxidation reflect an interplay of conformational, stereoelectronic, and inductive factors. PMID:19254041

Backscattered electron SEM imaging of resin sections from plant specimens: observation of histological to subcellular structure and CLEM.

PubMed

Rizzo, N W; Duncan, K E; Bourett, T M; Howard, R J

2016-08-01

We have refined methods for biological specimen preparation and low-voltage backscattered electron imaging in the scanning electron microscope that allow for observation at continuous magnifications of ca. 130-70 000 X, and documentation of tissue and subcellular ultrastructure detail. The technique, based upon early work by Ogura & Hasegawa (1980), affords use of significantly larger sections from fixed and resin-embedded specimens than is possible with transmission electron microscopy while providing similar data. After microtomy, the sections, typically ca. 750 nm thick, were dried onto the surface of glass or silicon wafer and stained with heavy metals-the use of grids avoided. The glass/wafer support was then mounted onto standard scanning electron microscopy sample stubs, carbon-coated and imaged directly at an accelerating voltage of 5 kV, using either a yttrium aluminum garnet or ExB backscattered electron detector. Alternatively, the sections could be viewed first by light microscopy, for example to document signal from a fluorescent protein, and then by scanning electron microscopy to provide correlative light/electron microscope (CLEM) data. These methods provide unobstructed access to ultrastructure in the spatial context of a section ca. 7 × 10 mm in size, significantly larger than the typical 0.2 × 0.3 mm section used for conventional transmission electron microscopy imaging. Application of this approach was especially useful when the biology of interest was rare or difficult to find, e.g. a particular cell type, developmental stage, large organ, the interface between cells of interacting organisms, when contextual information within a large tissue was obligatory, or combinations of these factors. In addition, the methods were easily adapted for immunolocalizations. © 2015 The Author. Journal of Microscopy published by John Wiley & Sons, Ltd on behalf of the Royal Microscopical Society.

Preservation of protein fluorescence in embedded human dendritic cells for targeted 3D light and electron microscopy.

PubMed

Höhn, K; Fuchs, J; Fröber, A; Kirmse, R; Glass, B; Anders-Össwein, M; Walther, P; Kräusslich, H-G; Dietrich, C

2015-08-01

In this study, we present a correlative microscopy workflow to combine detailed 3D fluorescence light microscopy data with ultrastructural information gained by 3D focused ion beam assisted scanning electron microscopy. The workflow is based on an optimized high pressure freezing/freeze substitution protocol that preserves good ultrastructural detail along with retaining the fluorescence signal in the resin embedded specimens. Consequently, cellular structures of interest can readily be identified and imaged by state of the art 3D confocal fluorescence microscopy and are precisely referenced with respect to an imprinted coordinate system on the surface of the resin block. This allows precise guidance of the focused ion beam assisted scanning electron microscopy and limits the volume to be imaged to the structure of interest. This, in turn, minimizes the total acquisition time necessary to conduct the time consuming ultrastructural scanning electron microscope imaging while eliminating the risk to miss parts of the target structure. We illustrate the value of this workflow for targeting virus compartments, which are formed in HIV-pulsed mature human dendritic cells. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

Integration of a high-NA light microscope in a scanning electron microscope.

PubMed

Zonnevylle, A C; Van Tol, R F C; Liv, N; Narvaez, A C; Effting, A P J; Kruit, P; Hoogenboom, J P

2013-10-01

We present an integrated light-electron microscope in which an inverted high-NA objective lens is positioned inside a scanning electron microscope (SEM). The SEM objective lens and the light objective lens have a common axis and focal plane, allowing high-resolution optical microscopy and scanning electron microscopy on the same area of a sample simultaneously. Components for light illumination and detection can be mounted outside the vacuum, enabling flexibility in the construction of the light microscope. The light objective lens can be positioned underneath the SEM objective lens during operation for sub-10 μm alignment of the fields of view of the light and electron microscopes. We demonstrate in situ epifluorescence microscopy in the SEM with a numerical aperture of 1.4 using vacuum-compatible immersion oil. For a 40-nm-diameter fluorescent polymer nanoparticle, an intensity profile with a FWHM of 380 nm is measured whereas the SEM performance is uncompromised. The integrated instrument may offer new possibilities for correlative light and electron microscopy in the life sciences as well as in physics and chemistry. © 2013 The Authors Journal of Microscopy © 2013 Royal Microscopical Society.

A spiking neural network model of 3D perception for event-based neuromorphic stereo vision systems

PubMed Central

Osswald, Marc; Ieng, Sio-Hoi; Benosman, Ryad; Indiveri, Giacomo

2017-01-01

Stereo vision is an important feature that enables machine vision systems to perceive their environment in 3D. While machine vision has spawned a variety of software algorithms to solve the stereo-correspondence problem, their implementation and integration in small, fast, and efficient hardware vision systems remains a difficult challenge. Recent advances made in neuromorphic engineering offer a possible solution to this problem, with the use of a new class of event-based vision sensors and neural processing devices inspired by the organizing principles of the brain. Here we propose a radically novel model that solves the stereo-correspondence problem with a spiking neural network that can be directly implemented with massively parallel, compact, low-latency and low-power neuromorphic engineering devices. We validate the model with experimental results, highlighting features that are in agreement with both computational neuroscience stereo vision theories and experimental findings. We demonstrate its features with a prototype neuromorphic hardware system and provide testable predictions on the role of spike-based representations and temporal dynamics in biological stereo vision processing systems. PMID:28079187

A spiking neural network model of 3D perception for event-based neuromorphic stereo vision systems.

PubMed

Osswald, Marc; Ieng, Sio-Hoi; Benosman, Ryad; Indiveri, Giacomo

2017-01-12

Stereo vision is an important feature that enables machine vision systems to perceive their environment in 3D. While machine vision has spawned a variety of software algorithms to solve the stereo-correspondence problem, their implementation and integration in small, fast, and efficient hardware vision systems remains a difficult challenge. Recent advances made in neuromorphic engineering offer a possible solution to this problem, with the use of a new class of event-based vision sensors and neural processing devices inspired by the organizing principles of the brain. Here we propose a radically novel model that solves the stereo-correspondence problem with a spiking neural network that can be directly implemented with massively parallel, compact, low-latency and low-power neuromorphic engineering devices. We validate the model with experimental results, highlighting features that are in agreement with both computational neuroscience stereo vision theories and experimental findings. We demonstrate its features with a prototype neuromorphic hardware system and provide testable predictions on the role of spike-based representations and temporal dynamics in biological stereo vision processing systems.

A spiking neural network model of 3D perception for event-based neuromorphic stereo vision systems

NASA Astrophysics Data System (ADS)

Osswald, Marc; Ieng, Sio-Hoi; Benosman, Ryad; Indiveri, Giacomo

2017-01-01

Stereo vision is an important feature that enables machine vision systems to perceive their environment in 3D. While machine vision has spawned a variety of software algorithms to solve the stereo-correspondence problem, their implementation and integration in small, fast, and efficient hardware vision systems remains a difficult challenge. Recent advances made in neuromorphic engineering offer a possible solution to this problem, with the use of a new class of event-based vision sensors and neural processing devices inspired by the organizing principles of the brain. Here we propose a radically novel model that solves the stereo-correspondence problem with a spiking neural network that can be directly implemented with massively parallel, compact, low-latency and low-power neuromorphic engineering devices. We validate the model with experimental results, highlighting features that are in agreement with both computational neuroscience stereo vision theories and experimental findings. We demonstrate its features with a prototype neuromorphic hardware system and provide testable predictions on the role of spike-based representations and temporal dynamics in biological stereo vision processing systems.

STEREO-IMPACT E/PO at NASA's Sun-Earth Day Event: Participation in Total Eclipse 2006 Webcast

NASA Astrophysics Data System (ADS)

Craig, N.; Peticolas, L. M.; Mendez, B. J.; Luhmann, J. G.; Higdon, R.

2006-05-01

The Solar Terrestrial Relations Observatory (STEREO) is planned for launch in late Summer 2006. STEREO will study the Sun with two spacecraft in orbit around the Sun moving on opposite sides of Earth. The primary science goal is to understand the nature of Coronal Mass Ejections (CMEs). This presentation will focus on one of the informal education efforts of our E/PO program for the IMPACT instrument suite aboard STEREO. We will share our participation in NASA's Sun-Earth Day event which is scheduled to coincide with a total solar eclipse in March and is titled In a Different Light. We will show how this live eclipse Webcast, which reaches thousands of science center attendees, can inspire the public to observe, understand and be part of the Sun-Earth-Moon system. We will present video clips of STEREO-IMPACT team members Janet Luhmann and Nahide Craig participating in the Exploratorium's live Webcast of the 2006 solar eclipse on location from Side, Turkey, and the experiences and remarks of the other STEREO scientist from the path of totality from Africa.

Bias Reduction and Filter Convergence for Long Range Stereo

NASA Technical Reports Server (NTRS)

Sibley, Gabe; Matthies, Larry; Sukhatme, Gaurav

2005-01-01

We are concerned here with improving long range stereo by filtering image sequences. Traditionally, measurement errors from stereo camera systems have been approximated as 3-D Gaussians, where the mean is derived by triangulation and the covariance by linearized error propagation. However, there are two problems that arise when filtering such 3-D measurements. First, stereo triangulation suffers from a range dependent statistical bias; when filtering this leads to over-estimating the true range. Second, filtering 3-D measurements derived via linearized error propagation leads to apparent filter divergence; the estimator is biased to under-estimate range. To address the first issue, we examine the statistical behavior of stereo triangulation and show how to remove the bias by series expansion. The solution to the second problem is to filter with image coordinates as measurements instead of triangulated 3-D coordinates.

KSC-06pd2263

NASA Image and Video Library

2006-10-10

KENNEDY SPACE CENTER, FLA. - With a convoy of escorts, the STEREO spacecraft is transported to Launch Pad 17-B on Cape Canaveral Air Force Station. At the pad the spacecraft will be lifted into the mobile service tower. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

A Comparative Study of Radar Stereo and Interferometry for DEM Generation

NASA Astrophysics Data System (ADS)

Gelautz, M.; Paillou, P.; Chen, C. W.; Zebker, H. A.

2004-06-01

In this experiment, we derive and compare radar stereo and interferometric elevation models (DEMs) of a study site in Djibouti, East Africa. As test data, we use a Radarsat stereo pair and ERS-2 and Radarsat interferometric data. Comparison of the reconstructed DEMs with a SPOT reference DEM shows that in regions of high coherence the DEMs produced by interferometry are of much better quality than the stereo result. However, the interferometric error histograms also show some pronounced outliers due to decorrelation and phase unwrapping problems on forested mountain slopes. The more robust stereo result is able to capture the general terrain shape, but finer surface details are lost. A fusion experiment demonstrates that merging the stereoscopic and interferometric DEMs by utilizing coherence- derived weights can significantly improve the accuracy of the computed elevation maps.

Electron microscopy approach for the visualization of the epithelial and endothelial glycocalyx.

PubMed

Chevalier, L; Selim, J; Genty, D; Baste, J M; Piton, N; Boukhalfa, I; Hamzaoui, M; Pareige, P; Richard, V

2017-06-01

This study presents a methodological approach for the visualization of the glycocalyx by electron microscopy. The glycocalyx is a three dimensional network mainly composed of glycolipids, glycoproteins and proteoglycans associated with the plasma membrane. Since less than a decade, the epithelial and endothelial glycocalyx proved to play an important role in physiology and pathology, increasing its research interest especially in vascular functions. Therefore, visualization of the glycocalyx requires reliable techniques and its preservation remains challenging due to its fragile and dynamic organization, which is highly sensitive to the different process steps for electron microscopy sampling. In this study, chemical fixation was performed by perfusion as a good alternative to conventional fixation. Additional lanthanum nitrate in the fixative enhances staining of the glycocalyx in transmission electron microscopy bright field and improves its visualization by detecting the elastic scattered electrons, thus providing a chemical contrast. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Scanning electron microscopy of cells and tissues under fully hydrated conditions

PubMed Central

Thiberge, Stephan; Nechushtan, Amotz; Sprinzak, David; Gileadi, Opher; Behar, Vered; Zik, Ory; Chowers, Yehuda; Michaeli, Shulamit; Schlessinger, Joseph; Moses, Elisha

2004-01-01

A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The resolution of low-contrast materials is ≈100 nm, whereas in high-contrast materials the resolution can reach 10 nm. Standard immunogold techniques and heavy-metal stains can be applied and viewed in the fluid to improve the contrast. Images present a striking combination of whole-cell morphology with a wealth of internal details. A possibility for direct inspection of tissue slices transpires, imaging only the external layer of cells. Simultaneous imaging with photons excited by the electrons incorporates data on material distribution, indicating a potential for multilabeling and specific scintillating markers. PMID:14988502

The stereo-dynamics of collisional autoionization of ammonia by helium and neon metastable excited atoms through molecular beam experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Falcinelli, Stefano, E-mail: stefano.falcinelli@unipg.it; Vecchiocattivi, Franco; Bartocci, Alessio

2015-10-28

A combined analysis of both new (energy spectra of emitted electrons) and previously published (ionization cross sections) experimental data, measured under the same conditions and concerning electronically excited lighter noble gas –NH{sub 3} collisional autoionization processes, is carried out. Such an analysis, performed by exploiting a formulation of the full potential energy surface both in the real and imaginary parts, provides direct information on energetics, structure, and lifetime of the intermediate collision complex over all the configuration space. The marked anisotropy in the attraction of the real part, driving the approach of reagents, and the selective role of the imaginarymore » component, associated to the charge transfer coupling between entrance and exit channels, suggests that reactive events occur almost exclusively in the molecular hemisphere containing the nitrogen lone pair. Crucial details on the stereo-dynamics of elementary collisional autoionization processes are then obtained, in which the open shell nature of the disclosed ionic core of metastable atom plays a crucial role. The same analysis also suggests that the strength of the attraction and the anisotropy of the interaction increases regularly along the series Ne{sup *}({sup 3}P), He{sup *}({sup 3}S), He{sup *}({sup 1}S)–NH{sub 3}. These findings can be ascribed to the strong rise of the metastable atom electronic polarizability (deformability) along the series. The obtained results can stimulate state of the art ab initio calculations focused on specific features of the transition state (energetics, structure, lifetime, etc.) which can be crucial for a further improvement of the adopted treatment and to better understand the nature of the leading interaction components which are the same responsible for the formation of the intermolecular halogen and hydrogen bond.« less

Attosecond electron pulse trains and quantum state reconstruction in ultrafast transmission electron microscopy

NASA Astrophysics Data System (ADS)

Priebe, Katharina E.; Rathje, Christopher; Yalunin, Sergey V.; Hohage, Thorsten; Feist, Armin; Schäfer, Sascha; Ropers, Claus

2017-12-01

Ultrafast electron and X-ray imaging and spectroscopy are the basis for an ongoing revolution in the understanding of dynamical atomic-scale processes in matter. The underlying technology relies heavily on laser science for the generation and characterization of ever shorter pulses. Recent findings suggest that ultrafast electron microscopy with attosecond-structured wavefunctions may be feasible. However, such future technologies call for means to both prepare and fully analyse the corresponding free-electron quantum states. Here, we introduce a framework for the preparation, coherent manipulation and characterization of free-electron quantum states, experimentally demonstrating attosecond electron pulse trains. Phase-locked optical fields coherently control the electron wavefunction along the beam direction. We establish a new variant of quantum state tomography—`SQUIRRELS'—for free-electron ensembles. The ability to tailor and quantitatively map electron quantum states will promote the nanoscale study of electron-matter entanglement and new forms of ultrafast electron microscopy down to the attosecond regime.

Nanowire growth kinetics in aberration corrected environmental transmission electron microscopy

DOE PAGES

Chou, Yi -Chia; Panciera, Federico; Reuter, Mark C.; ...

2016-03-15

Here, we visualize atomic level dynamics during Si nanowire growth using aberration corrected environmental transmission electron microscopy, and compare with lower pressure results from ultra-high vacuum microscopy. We discuss the importance of higher pressure observations for understanding growth mechanisms and describe protocols to minimize effects of the higher pressure background gas.

Electronic Blending in Virtual Microscopy

ERIC Educational Resources Information Center

Maybury, Terrence S.; Farah, Camile S.

2010-01-01

Virtual microscopy (VM) is a relatively new technology that transforms the computer into a microscope. In essence, VM allows for the scanning and transfer of glass slides from light microscopy technology to the digital environment of the computer. This transition is also a function of the change from print knowledge to electronic knowledge, or as…

Structural and Ultrastructural Characteristics of Bone-Tendon Junction of the Calcaneal Tendon of Adult and Elderly Wistar Rats

PubMed Central

Cury, Diego Pulzatto; Dias, Fernando José; Miglino, Maria Angélica; Watanabe, Ii-sei

2016-01-01

Tendons are transition tissues that transfer the contractile forces generated by the muscles to the bones, allowing movement. The region where the tendon attaches to the bone is called bone-tendon junction or enthesis and may be classified as fibrous or fibrocartilaginous. This study aims to analyze the collagen fibers and the cells present in the bone-tendon junction using light microscopy and ultrastructural techniques as scanning electron microscopy and transmission electron microscopy. Forty male Wistar rats were used in the experiment, being 20 adult rats at 4 months-old and 20 elderly rats at 20 months-old. The hind limbs of the rats were removed, dissected and prepared to light microscopy, transmission electron microscopy and scanning electron microscopy. The aging process showed changes in the collagen fibrils, with a predominance of type III fibers in the elderly group, in addition to a decrease in the amount of the fibrocartilage cells, fewer and shorter cytoplasmic processes and a decreased synthetic capacity due to degradation of the organelles involved in synthesis. PMID:27078690

Preservation of protein fluorescence in embedded human dendritic cells for targeted 3D light and electron microscopy

PubMed Central

HÖHN, K.; FUCHS, J.; FRÖBER, A.; KIRMSE, R.; GLASS, B.; ANDERS‐ÖSSWEIN, M.; WALTHER, P.; KRÄUSSLICH, H.‐G.

2015-01-01

Summary In this study, we present a correlative microscopy workflow to combine detailed 3D fluorescence light microscopy data with ultrastructural information gained by 3D focused ion beam assisted scanning electron microscopy. The workflow is based on an optimized high pressure freezing/freeze substitution protocol that preserves good ultrastructural detail along with retaining the fluorescence signal in the resin embedded specimens. Consequently, cellular structures of interest can readily be identified and imaged by state of the art 3D confocal fluorescence microscopy and are precisely referenced with respect to an imprinted coordinate system on the surface of the resin block. This allows precise guidance of the focused ion beam assisted scanning electron microscopy and limits the volume to be imaged to the structure of interest. This, in turn, minimizes the total acquisition time necessary to conduct the time consuming ultrastructural scanning electron microscope imaging while eliminating the risk to miss parts of the target structure. We illustrate the value of this workflow for targeting virus compartments, which are formed in HIV‐pulsed mature human dendritic cells. PMID:25786567

Structural and Ultrastructural Characteristics of Bone-Tendon Junction of the Calcaneal Tendon of Adult and Elderly Wistar Rats.

PubMed

Cury, Diego Pulzatto; Dias, Fernando José; Miglino, Maria Angélica; Watanabe, Ii-sei

2016-01-01

Tendons are transition tissues that transfer the contractile forces generated by the muscles to the bones, allowing movement. The region where the tendon attaches to the bone is called bone-tendon junction or enthesis and may be classified as fibrous or fibrocartilaginous. This study aims to analyze the collagen fibers and the cells present in the bone-tendon junction using light microscopy and ultrastructural techniques as scanning electron microscopy and transmission electron microscopy. Forty male Wistar rats were used in the experiment, being 20 adult rats at 4 months-old and 20 elderly rats at 20 months-old. The hind limbs of the rats were removed, dissected and prepared to light microscopy, transmission electron microscopy and scanning electron microscopy. The aging process showed changes in the collagen fibrils, with a predominance of type III fibers in the elderly group, in addition to a decrease in the amount of the fibrocartilage cells, fewer and shorter cytoplasmic processes and a decreased synthetic capacity due to degradation of the organelles involved in synthesis.

Stereo vision with distance and gradient recognition

NASA Astrophysics Data System (ADS)

Kim, Soo-Hyun; Kang, Suk-Bum; Yang, Tae-Kyu

2007-12-01

Robot vision technology is needed for the stable walking, object recognition and the movement to the target spot. By some sensors which use infrared rays and ultrasonic, robot can overcome the urgent state or dangerous time. But stereo vision of three dimensional space would make robot have powerful artificial intelligence. In this paper we consider about the stereo vision for stable and correct movement of a biped robot. When a robot confront with an inclination plane or steps, particular algorithms are needed to go on without failure. This study developed the recognition algorithm of distance and gradient of environment by stereo matching process.

Three-dimensional displays and stereo vision

PubMed Central

Westheimer, Gerald

2011-01-01

Procedures for three-dimensional image reconstruction that are based on the optical and neural apparatus of human stereoscopic vision have to be designed to work in conjunction with it. The principal methods of implementing stereo displays are described. Properties of the human visual system are outlined as they relate to depth discrimination capabilities and achieving optimal performance in stereo tasks. The concept of depth rendition is introduced to define the change in the parameters of three-dimensional configurations for cases in which the physical disposition of the stereo camera with respect to the viewed object differs from that of the observer's eyes. PMID:21490023

KSC-06pd1552

NASA Image and Video Library

2006-07-13

KENNEDY SPACE CENTER, FLA. - Inside the mobile service tower on Launch Pad 17-B on Cape Canaveral Air Force Station, workers stand by as the Boeing Delta II second stage for the STEREO launch is lowered onto the first stage for mating. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off in August 2006. Photo credit: NASA/George Shelton

KSC-06pd1858

NASA Image and Video Library

2006-08-10

KENNEDY SPACE CENTER, FLA. - Technicians inside the Astrotech facility in Titusville, Florida, move the STEREO spacecraft to the spin table. The twin observatories will undergo a spin test to check balance and alignment in preparation for flight. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1538

NASA Image and Video Library

2006-07-10

KENNEDY SPACE CENTER, FLA. - In the hazardous processing facility at Astrotech Space Operations in Titusville, Fla., technicians check Observatory A before lifting onto a scale for weight measurements. The observatory is one of two in the STEREO spacecraft and later will be fueled. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket no earlier than Aug. 1. Photo credit: NASA/Jack Pfaller

KSC-06pd1742

NASA Image and Video Library

2006-08-05

KENNEDY SPACE CENTER, FLA. - Inside the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, the second stage of the Boeing Delta II launch vehicle for the STEREO spacecraft is being remated with the Delta first stage. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31. Photo credit: NASA/Dimitri Gerondidakis

KSC-06pd1887

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., workers check the fitting of the upper portion of the transportation canister onto the lower portion. The canister encases the STEREO spacecraft for its move to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

KSC-06pd1854

NASA Image and Video Library

2006-08-10

KENNEDY SPACE CENTER, FLA. - The STEREO spacecraft sits on a test stand inside the Astrotech facility in Titusville, Florida. The twin observatories will undergo a spin test to check balance and alignment in preparation for flight. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1541

NASA Image and Video Library

2006-07-10

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., technicians are ready to wrap more plastic around STEREO's Observatory B before its transfer to the hazardous processing facility where it will be weighed and fueled. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket no earlier than Aug. 1. Photo credit: NASA/George Shelton

KSC-06pd1880

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., workers move the stand holding the STEREO spacecraft and upper stage booster. The entire configuration will be encased for the move to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

KSC-06pd1883

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the upper portion of the transportation canister is lowered over the STEREO spacecraft (in front). When the entire configuration is encased, it will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

KSC-06pd1886

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., workers help guide the upper portion of the transportation canister onto the lower portion. The canister encases the STEREO spacecraft for its move to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

KSC-06pd1882

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the upper portion of the transportation canister is lowered over the STEREO spacecraft (in front). When the entire configuration is encased, it will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

KSC-06pd1551

NASA Image and Video Library

2006-07-13

KENNEDY SPACE CENTER, FLA. - Inside the mobile service tower on Launch Pad 17-B on Cape Canaveral Air Force Station, the Boeing Delta II second stage for the STEREO launch is lowered toward the first stage. The two stages will be mated. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off in August 2006. Photo credit: NASA/George Shelton

Biological applications of phase-contrast electron microscopy.

PubMed

Nagayama, Kuniaki

2014-01-01

Here, I review the principles and applications of phase-contrast electron microscopy using phase plates. First, I develop the principle of phase contrast based on a minimal model of microscopy, introducing a double Fourier-transform process to mathematically formulate the image formation. Next, I explain four phase-contrast (PC) schemes, defocus PC, Zernike PC, Hilbert differential contrast, and schlieren optics, as image-filtering processes in the context of the minimal model, with particular emphases on the Zernike PC and corresponding Zernike phase plates. Finally, I review applications of Zernike PC cryo-electron microscopy to biological systems such as protein molecules, virus particles, and cells, including single-particle analysis to delineate three-dimensional (3D) structures of protein and virus particles and cryo-electron tomography to reconstruct 3D images of complex protein systems and cells.

Modified Test Protocol Improves Sensitivity of the Stereo Fly Test.

PubMed

De La Cruz, Angie; Morale, Sarah E; Jost, Reed M; Kelly, Krista R; Birch, Eileen E

2016-01-01

Stereoacuity measurement is a common element of pediatric ophthalmic examinations. Although the Stereo Fly Test is routinely used to establish the presence of coarse stereopsis (3000 arcsecs), it often yields a false negative "pass" due to learned responses and non-stereoscopic cues. We developed and evaluated a modified Stereo Fly Test protocol aimed at increasing sensitivity, thus reducing false negatives. The Stereo Fly Test was administered according to manufacturer instructions to 321 children aged 3-12 years. Children with a "pass" outcome (n = 147) were re-tested wearing glasses fitted with polarizers of matching orientation for both eyes to verify that they were responding to stereoscopic cues (modified protocol). The response to the standard Stereo Fly Test was considered a false negative (pass) if the child still pinched above the plate after disparity cues were eliminated. Randot ® Preschool Stereoacuity and Butterfly Tests were used as gold standards. Sensitivity was 81% (95% CI: 0.75 - 0.86) for standard administration of the Stereo Fly Test (19% false negative "pass"). The modified protocol increased sensitivity to 90% (95% CI: 0.85 - 0.94). The modified two-step protocol is a simple and convenient way to administer the Stereo Fly Test with increased sensitivity in a clinical setting. © 2016 Board of regents of the University of Wisconsin System, American Orthoptic Journal, Volume 66, 2016, ISSN 0065-955X, E-ISSN 1553-4448.

Stereo ENA Imaging of the Ring Current and Multi-point Measurements of Suprathermal Particles and Magnetic Fields by TRIO-CINEMA

NASA Astrophysics Data System (ADS)

Lin, R. P.; Sample, J. G.; Immel, T. J.; Lee, D.; Horbury, T. S.; Jin, H.; SEON, J.; Wang, L.; Roelof, E. C.; Lee, E.; Parks, G. K.; Vo, H.

2012-12-01

The TRIO (Triplet Ionospheric Observatory) - CINEMA (Cubesat for Ions, Neutrals, Electrons, & Magnetic fields) mission consists of three identical 3-u cubesats to provide high sensitivity, high cadence, stereo measurements of Energetic Neutral Atoms (ENAs) from the Earth's ring current with ~1 keV FWHM energy resolution from ~4 to ~200 keV, as well as multi-point in situ measurements of magnetic fields and suprathermal electrons (~2 -200 keV) and ions (~ 4 -200 keV) in the auroral and ring current precipitation regions in low Earth orbit (LEO). A new Suprathermal Electron, Ion, Neutral (STEIN) instrument, using a 32-pixel silicon semiconductor detector with an electrostatic deflection system to separate ENAs from ions and from electrons below 30 keV, will sweep over most of the sky every 15 s as the spacecraft spins at 4 rpm. In addition, inboard and outboard (on an extendable 1m boom) miniature magnetoresistive sensor magnetometers will provide high cadence 3-axis magnetic field measurements. An S-band transmitter will be used to provide ~8 kbps orbit-average data downlink to the ~11m diameter antenna of the Berkeley Ground Station.The first CINEMA (funded by NSF) is scheduled for launch on August 14, 2012 into a 65 deg. inclination LEO. Two more identical CINEMAs are being developed by Kyung Hee University (KHU) in Korea under the World Class University (WCU) program, for launch in November 2012 into a Sun-synchronous LEO to form TRIO-CINEMA. A fourth CINEMA is being developed for a 2013 launch into LEO. This LEO constellation of nanosatellites will provide unique measurements highly complementary to NASA's RBSP and THEMIS missions. Furthermore, CINEMA's development of miniature particle and magnetic field sensors, and cubesat-size spinning spacecraft may be important for future constellation space missions. Initial results from the first CINEMA will be presented if available.

Engineering cofactor flexibility enhanced 2,3-butanediol production in Escherichia coli.

PubMed

Liang, Keming; Shen, Claire R

2017-12-01

Enzymatic reduction of acetoin into 2,3-butanediol (2,3-BD) typically requires the reduced nicotinamide adenine dinucleotide (NADH) or its phosphate form (NADPH) as electron donor. Efficiency of 2,3-BD biosynthesis, therefore, is heavily influenced by the enzyme specificity and the cofactor availability which varies dynamically. This work describes the engineering of cofactor flexibility for 2,3-BD production by simultaneous overexpression of an NADH-dependent 2,3-BD dehydrogenase from Klebsiella pneumoniae (KpBudC) and an NADPH-specific 2,3-BD dehydrogenase from Clostridium beijerinckii (CbAdh). Co-expression of KpBudC and CbAdh not only enabled condition versatility for 2,3-BD synthesis via flexible utilization of cofactors, but also improved production stereo-specificity of 2,3-BD without accumulation of acetoin. With optimization of medium and fermentation condition, the co-expression strain produced 92 g/L of 2,3-BD in 56 h with 90% stereo-purity for (R,R)-isoform and 85% of maximum theoretical yield. Incorporating cofactor flexibility into the design principle should benefit production of bio-based chemical involving redox reactions.

ON THE BRIGHTNESS AND WAITING-TIME DISTRIBUTIONS OF A TYPE III RADIO STORM OBSERVED BY STEREO/WAVES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eastwood, J. P.; Hudson, H. S.; Krucker, S.

2010-01-10

Type III solar radio storms, observed at frequencies below {approx}16 MHz by space-borne radio experiments, correspond to the quasi-continuous, bursty emission of electron beams onto open field lines above active regions. The mechanisms by which a storm can persist in some cases for more than a solar rotation whilst exhibiting considerable radio activity are poorly understood. To address this issue, the statistical properties of a type III storm observed by the STEREO/WAVES radio experiment are presented, examining both the brightness distribution and (for the first time) the waiting-time distribution (WTD). Single power-law behavior is observed in the number distribution asmore » a function of brightness; the power-law index is {approx}2.1 and is largely independent of frequency. The WTD is found to be consistent with a piecewise-constant Poisson process. This indicates that during the storm individual type III bursts occur independently and suggests that the storm dynamics are consistent with avalanche-type behavior in the underlying active region.« less

FM Stereo and AM Stereo: Government Standard-Setting vs. the Marketplace.

ERIC Educational Resources Information Center

Huff, W. A. Kelly

The emergence of frequency modulation or FM radio signals, which arose from the desire to free broadcasting of static noise common to amplitude modulation or AM, has produced the controversial development of stereo broadcasting. The resulting enhancement of sound quality helped FM pass AM in audience shares in less than two decades. The basic…

Viking orbiter stereo imaging catalog

NASA Technical Reports Server (NTRS)

Blasius, K. R.; Vertrone, A. V.; Lewis, B. H.; Martin, M. D.

1982-01-01

The extremely long mission of the two Viking Orbiter spacecraft produced a wealth of photos of surface features. Many of these photos can be used to form stereo images allowing the student of Mars to examine a subject in three dimensional. This catalog is a technical guide to the use of stereo coverage within the complex Viking imaging data set.

Fast electron microscopy via compressive sensing

DOEpatents

Larson, Kurt W; Anderson, Hyrum S; Wheeler, Jason W

2014-12-09

Various technologies described herein pertain to compressive sensing electron microscopy. A compressive sensing electron microscope includes a multi-beam generator and a detector. The multi-beam generator emits a sequence of electron patterns over time. Each of the electron patterns can include a plurality of electron beams, where the plurality of electron beams is configured to impart a spatially varying electron density on a sample. Further, the spatially varying electron density varies between each of the electron patterns in the sequence. Moreover, the detector collects signals respectively corresponding to interactions between the sample and each of the electron patterns in the sequence.

Photon gating in four-dimensional ultrafast electron microscopy.

PubMed

Hassan, Mohammed T; Liu, Haihua; Baskin, John Spencer; Zewail, Ahmed H

2015-10-20

Ultrafast electron microscopy (UEM) is a pivotal tool for imaging of nanoscale structural dynamics with subparticle resolution on the time scale of atomic motion. Photon-induced near-field electron microscopy (PINEM), a key UEM technique, involves the detection of electrons that have gained energy from a femtosecond optical pulse via photon-electron coupling on nanostructures. PINEM has been applied in various fields of study, from materials science to biological imaging, exploiting the unique spatial, energy, and temporal characteristics of the PINEM electrons gained by interaction with a "single" light pulse. The further potential of photon-gated PINEM electrons in probing ultrafast dynamics of matter and the optical gating of electrons by invoking a "second" optical pulse has previously been proposed and examined theoretically in our group. Here, we experimentally demonstrate this photon-gating technique, and, through diffraction, visualize the phase transition dynamics in vanadium dioxide nanoparticles. With optical gating of PINEM electrons, imaging temporal resolution was improved by a factor of 3 or better, being limited only by the optical pulse widths. This work enables the combination of the high spatial resolution of electron microscopy and the ultrafast temporal response of the optical pulses, which provides a promising approach to attain the resolution of few femtoseconds and attoseconds in UEM.

Photon gating in four-dimensional ultrafast electron microscopy

PubMed Central

Hassan, Mohammed T.; Liu, Haihua; Baskin, John Spencer; Zewail, Ahmed H.

2015-01-01

Ultrafast electron microscopy (UEM) is a pivotal tool for imaging of nanoscale structural dynamics with subparticle resolution on the time scale of atomic motion. Photon-induced near-field electron microscopy (PINEM), a key UEM technique, involves the detection of electrons that have gained energy from a femtosecond optical pulse via photon–electron coupling on nanostructures. PINEM has been applied in various fields of study, from materials science to biological imaging, exploiting the unique spatial, energy, and temporal characteristics of the PINEM electrons gained by interaction with a “single” light pulse. The further potential of photon-gated PINEM electrons in probing ultrafast dynamics of matter and the optical gating of electrons by invoking a “second” optical pulse has previously been proposed and examined theoretically in our group. Here, we experimentally demonstrate this photon-gating technique, and, through diffraction, visualize the phase transition dynamics in vanadium dioxide nanoparticles. With optical gating of PINEM electrons, imaging temporal resolution was improved by a factor of 3 or better, being limited only by the optical pulse widths. This work enables the combination of the high spatial resolution of electron microscopy and the ultrafast temporal response of the optical pulses, which provides a promising approach to attain the resolution of few femtoseconds and attoseconds in UEM. PMID:26438835

Left Limb of North Pole of the Sun, March 20, 2007 (Anaglyph)

NASA Technical Reports Server (NTRS)

2007-01-01

[figure removed for brevity, see original site] [figure removed for brevity, see original site] Figure 1: Left eye view of a stereo pair Click on the image for full resolution TIFF Figure 2: Right eye view of a stereo pair Click on the image for full resolution TIFF Figure 1: This image was taken by the SECCHI Extreme UltraViolet Imager (EUVI) mounted on the STEREO-B spacecraft. STEREO-B is located behind the Earth, and follows the Earth in orbit around the Sun. This location enables us to view the Sun from the position of a virtual left eye in space. Figure 2: This image was taken by the SECCHI Extreme UltraViolet Imager (EUVI) mounted on the STEREO-A spacecraft. STEREO-A is located ahead of the Earth, and leads the Earth in orbit around the Sun, This location enables us to view the Sun from the position of a virtual right eye in space.

NASA's Solar TErrestrial RElations Observatory (STEREO) satellites have provided the first three-dimensional images of the Sun. For the first time, scientists will be able to see structures in the Sun's atmosphere in three dimensions. The new view will greatly aid scientists' ability to understand solar physics and thereby improve space weather forecasting.

This image is a composite of left and right eye color image pairs taken by the SECCHI Extreme UltraViolet Imager (EUVI) mounted on the STEREO-B and STEREO-A spacecraft. STEREO-B is located behind the Earth, and follows the Earth in orbit around the Sun, This location enables us to view the Sun from the position of a virtual left eye in space. STEREO-A is located ahead of the Earth, and leads the Earth in orbit around the Sun, This location enables us to view the Sun from the position of a virtual right eye in space.

The EUVI imager is sensitive to wavelengths of light in the extreme ultraviolet portion of the spectrum. EUVI bands at wavelengths of 304, 171 and 195 Angstroms have been mapped to the red blue and green visible portion of the spectrum; and processed to emphasize the three-dimensional structure of the solar material.

STEREO, a two-year mission, launched October 2006, will provide a unique and revolutionary view of the Sun-Earth System. The two nearly identical observatories -- one ahead of Earth in its orbit, the other trailing behind -- will trace the flow of energy and matter from the Sun to Earth. They will reveal the 3D structure of coronal mass ejections; violent eruptions of matter from the sun that can disrupt satellites and power grids, and help us understand why they happen. STEREO will become a key addition to the fleet of space weather detection satellites by providing more accurate alerts for the arrival time of Earth-directed solar ejections with its unique side-viewing perspective.

STEREO is the third mission in NASA's Solar Terrestrial Probes program within NASA's Science Mission Directorate, Washington. The Goddard Science and Exploration Directorate manages the mission, instruments, and science center. The Johns Hopkins University Applied Physics Laboratory, Laurel, Md., designed and built the spacecraft and is responsible for mission operations. The imaging and particle detecting instruments were designed and built by scientific institutions in the U.S., UK, France, Germany, Belgium, Netherlands, and Switzerland. JPL is a division of the California Institute of Technology in Pasadena.

High yield production of long branched Au nanoparticles characterized by atomic resolution transmission electron microscopy

PubMed Central

Mayoral, Alvaro; Magen, Cesar; Jose-Yacaman, Miguel

2011-01-01

Long multi-branched gold nanoparticles have been synthesized in a very high yield through a facile synthesis combining two different capping agents. The stability of these materials with the time has been tested and their characterization have been performed by diverse advanced electron microscopy techniques, paying special attention to aberration corrected transmission electron microscopy in order to unambiguously analyze the surface structure of the branches and provide insights for the formation of stellated gold nanoparticles. PMID:22125420

The Beagle 2 Stereo Camera System: Scientific Objectives and Design Characteristics

NASA Astrophysics Data System (ADS)

Griffiths, A.; Coates, A.; Josset, J.; Paar, G.; Sims, M.

2003-04-01

The Stereo Camera System (SCS) will provide wide-angle (48 degree) multi-spectral stereo imaging of the Beagle 2 landing site in Isidis Planitia with an angular resolution of 0.75 milliradians. Based on the SpaceX Modular Micro-Imager, the SCS is composed of twin cameras (with 1024 by 1024 pixel frame transfer CCD) and twin filter wheel units (with a combined total of 24 filters). The primary mission objective is to construct a digital elevation model of the area in reach of the lander’s robot arm. The SCS specifications and following baseline studies are described: Panoramic RGB colour imaging of the landing site and panoramic multi-spectral imaging at 12 distinct wavelengths to study the mineralogy of landing site. Solar observations to measure water vapour absorption and the atmospheric dust optical density. Also envisaged are multi-spectral observations of Phobos &Deimos (observations of the moons relative to background stars will be used to determine the lander’s location and orientation relative to the Martian surface), monitoring of the landing site to detect temporal changes, observation of the actions and effects of the other PAW experiments (including rock texture studies with a close-up-lens) and collaborative observations with the Mars Express orbiter instrument teams. Due to be launched in May of this year, the total system mass is 360 g, the required volume envelope is 747 cm^3 and the average power consumption is 1.8 W. A 10Mbit/s RS422 bus connects each camera to the lander common electronics.

Using Combination of Planar and Height Features for Detecting Built-Up Areas from High-Resolution Stereo Imagery

NASA Astrophysics Data System (ADS)

Peng, F.; Cai, X.; Tan, W.

2017-09-01

Within-class spectral variation and between-class spectral confusion in remotely sensed imagery degrades the performance of built-up area detection when using planar texture, shape, and spectral features. Terrain slope and building height are often used to optimize the results, but extracted from auxiliary data (e.g. LIDAR data, DSM). Moreover, the auxiliary data must be acquired around the same time as image acquisition. Otherwise, built-up area detection accuracy is affected. Stereo imagery incorporates both planar and height information unlike single remotely sensed images. Stereo imagery acquired by many satellites (e.g. Worldview-4, Pleiades-HR, ALOS-PRISM, and ZY-3) can be used as data source of identifying built-up areas. A new method of identifying high-accuracy built-up areas from stereo imagery is achieved by using a combination of planar and height features. The digital surface model (DSM) and digital orthophoto map (DOM) are first generated from stereo images. Then, height values of above-ground objects (e.g. buildings) are calculated from the DSM, and used to obtain raw built-up areas. Other raw built-up areas are obtained from the DOM using Pantex and Gabor, respectively. Final high-accuracy built-up area results are achieved from these raw built-up areas using the decision level fusion. Experimental results show that accurate built-up areas can be achieved from stereo imagery. The height information used in the proposed method is derived from stereo imagery itself, with no need to require auxiliary height data (e.g. LIDAR data). The proposed method is suitable for spaceborne and airborne stereo pairs and triplets.

WASS: An open-source pipeline for 3D stereo reconstruction of ocean waves

NASA Astrophysics Data System (ADS)

Bergamasco, Filippo; Torsello, Andrea; Sclavo, Mauro; Barbariol, Francesco; Benetazzo, Alvise

2017-10-01

Stereo 3D reconstruction of ocean waves is gaining more and more popularity in the oceanographic community and industry. Indeed, recent advances of both computer vision algorithms and computer processing power now allow the study of the spatio-temporal wave field with unprecedented accuracy, especially at small scales. Even if simple in theory, multiple details are difficult to be mastered for a practitioner, so that the implementation of a sea-waves 3D reconstruction pipeline is in general considered a complex task. For instance, camera calibration, reliable stereo feature matching and mean sea-plane estimation are all factors for which a well designed implementation can make the difference to obtain valuable results. For this reason, we believe that the open availability of a well tested software package that automates the reconstruction process from stereo images to a 3D point cloud would be a valuable addition for future researches in this area. We present WASS (http://www.dais.unive.it/wass), an Open-Source stereo processing pipeline for sea waves 3D reconstruction. Our tool completely automates all the steps required to estimate dense point clouds from stereo images. Namely, it computes the extrinsic parameters of the stereo rig so that no delicate calibration has to be performed on the field. It implements a fast 3D dense stereo reconstruction procedure based on the consolidated OpenCV library and, lastly, it includes set of filtering techniques both on the disparity map and the produced point cloud to remove the vast majority of erroneous points that can naturally arise while analyzing the optically complex nature of the water surface. In this paper, we describe the architecture of WASS and the internal algorithms involved. The pipeline workflow is shown step-by-step and demonstrated on real datasets acquired at sea.

Current STEREO Status on the Far Side of the Sun

NASA Astrophysics Data System (ADS)

Thompson, William T.; Gurman, Joseph; Ossing, Daniel; Luhmann, Janet; Curtis, David; Schroeder, Peter; Mewaldt, Richard; Davis, Andrew; Wortman, Kristin; Russell, Christopher; Galvin, Antoinette; Kistler, Lynn; Ellis, Lorna; Howard, Russell; Vourlidas, Angelos; Rich, Nathan; Hutting, Lynn; Maksimovic, Milan; Bale, Stuart D.; Goetz, Keith

2015-04-01

The current positions of the two STEREO spacecraft on the opposite side of the Sun from Earth (superior solar conjunction) has forced some significant changes in the spacecraft and instrument operations. No communications are possible when the spacecraft is within 2 degrees of the Sun, requiring that the spacecraft be put into safe mode until communications can be restored. Unfortunately, communications were lost with the STEREO Behind spacecraft on October 1, 2014, during testing for superior solar conjunction operations. We will discuss what is known about the causes of loss of contact, the steps being taken to try to recover the Behind spacecraft, and what has been done to prevent a similar occurrence on STEREO Ahead.We will also discuss the effect of being on the far side of the Sun on the science operations of STEREO Ahead. Starting on August 20, 2014, the telemetry rate from the STEREO Ahead spacecraft has been tremendously reduced due to the need to keep the temperature of the feed horn on the high gain antenna below acceptable limits. However, the amount of telemetry that can be brought down has been highly reduced. Even so, significant science is still possible from STEREO's unique position on the solar far side. We will discuss the science and space weather products that are, or will be, available from each STEREO instrument, when those products will be available, and how they will be used. Some data, including the regular space weather beacon products, are brought down for an average of a few hours each day during the daily real-time passes, while the in situ and radio beacon data are being stored on the onboard recorder to provide a continuous 24-hour coverage for eventual downlink once the spacecraft is back to normal operations.

Comparison of different "along the track" high resolution satellite stereo-pair for DSM extraction

NASA Astrophysics Data System (ADS)

Nikolakopoulos, Konstantinos G.

2013-10-01

The possibility to create DEM from stereo pairs is based on the Pythagoras theorem and on the principles of photogrammetry that are applied to aerial photographs stereo pairs for the last seventy years. The application of these principles to digital satellite stereo data was inherent in the first satellite missions. During the last decades the satellite stereo-pairs were acquired across the track in different days (SPOT, ERS etc.). More recently the same-date along the track stereo-data acquisition seems to prevail (Terra ASTER, SPOT5 HRS, Cartosat, ALOS Prism) as it reduces the radiometric image variations (refractive effects, sun illumination, temporal changes) and thus increases the correlation success rate in any image matching.Two of the newest satellite sensors with stereo collection capability is Cartosat and ALOS Prism. Both of them acquire stereopairs along the track with a 2,5m spatial resolution covering areas of 30X30km. In this study we compare two different satellite stereo-pair collected along the track for DSM creation. The first one is created from a Cartosat stereopair and the second one from an ALOS PRISM triplet. The area of study is situated in Chalkidiki Peninsula, Greece. Both DEMs were created using the same ground control points collected with a Differential GPS. After a first control for random or systematic errors a statistical analysis was done. Points of certified elevation have been used to estimate the accuracy of these two DSMs. The elevation difference between the different DEMs was calculated. 2D RMSE, correlation and the percentile value were also computed and the results are presented.

Cloud photogrammetry with dense stereo for fisheye cameras

NASA Astrophysics Data System (ADS)

Beekmans, Christoph; Schneider, Johannes; Läbe, Thomas; Lennefer, Martin; Stachniss, Cyrill; Simmer, Clemens

2016-11-01

We present a novel approach for dense 3-D cloud reconstruction above an area of 10 × 10 km2 using two hemispheric sky imagers with fisheye lenses in a stereo setup. We examine an epipolar rectification model designed for fisheye cameras, which allows the use of efficient out-of-the-box dense matching algorithms designed for classical pinhole-type cameras to search for correspondence information at every pixel. The resulting dense point cloud allows to recover a detailed and more complete cloud morphology compared to previous approaches that employed sparse feature-based stereo or assumed geometric constraints on the cloud field. Our approach is very efficient and can be fully automated. From the obtained 3-D shapes, cloud dynamics, size, motion, type and spacing can be derived, and used for radiation closure under cloudy conditions, for example. Fisheye lenses follow a different projection function than classical pinhole-type cameras and provide a large field of view with a single image. However, the computation of dense 3-D information is more complicated and standard implementations for dense 3-D stereo reconstruction cannot be easily applied. Together with an appropriate camera calibration, which includes internal camera geometry, global position and orientation of the stereo camera pair, we use the correspondence information from the stereo matching for dense 3-D stereo reconstruction of clouds located around the cameras. We implement and evaluate the proposed approach using real world data and present two case studies. In the first case, we validate the quality and accuracy of the method by comparing the stereo reconstruction of a stratocumulus layer with reflectivity observations measured by a cloud radar and the cloud-base height estimated from a Lidar-ceilometer. The second case analyzes a rapid cumulus evolution in the presence of strong wind shear.

Rapid matching of stereo vision based on fringe projection profilometry

NASA Astrophysics Data System (ADS)

Zhang, Ruihua; Xiao, Yi; Cao, Jian; Guo, Hongwei

2016-09-01

As the most important core part of stereo vision, there are still many problems to solve in stereo matching technology. For smooth surfaces on which feature points are not easy to extract, this paper adds a projector into stereo vision measurement system based on fringe projection techniques, according to the corresponding point phases which extracted from the left and right camera images are the same, to realize rapid matching of stereo vision. And the mathematical model of measurement system is established and the three-dimensional (3D) surface of the measured object is reconstructed. This measurement method can not only broaden application fields of optical 3D measurement technology, and enrich knowledge achievements in the field of optical 3D measurement, but also provide potential possibility for the commercialized measurement system in practical projects, which has very important scientific research significance and economic value.

Analysis and design of stereoscopic display in stereo television endoscope system

NASA Astrophysics Data System (ADS)

Feng, Dawei

2008-12-01

Many 3D displays have been proposed for medical use. When we design and evaluate new system, there are three demands from surgeons. Priority is the precision. Secondly, displayed images should be easy to understand, In addition, surgery lasts hours and hours, they do not like fatiguing display. The stereo television endoscope researched in this paper make celiac viscera image on the photosurface of the left and right CCD by imitating human binocular stereo vision effect by using the double-optical lines system. The left and right video signal will be processed by frequency multiplication and display on the monitor, people can observe the stereo image which has depth impression by using a polarized LCD screen and a pair of polarized glasses. Clinical experiments show that by using the stereo TV endoscope people can make minimally invasive surgery more safe and reliable, and can shorten the operation time, and can improve the operation accuracy.

Attenuating Stereo Pixel-Locking via Affine Window Adaptation

NASA Technical Reports Server (NTRS)

Stein, Andrew N.; Huertas, Andres; Matthies, Larry H.

2006-01-01

For real-time stereo vision systems, the standard method for estimating sub-pixel stereo disparity given an initial integer disparity map involves fitting parabolas to a matching cost function aggregated over rectangular windows. This results in a phenomenon known as 'pixel-locking,' which produces artificially-peaked histograms of sub-pixel disparity. These peaks correspond to the introduction of erroneous ripples or waves in the 3D reconstruction of truly Rat surfaces. Since stereo vision is a common input modality for autonomous vehicles, these inaccuracies can pose a problem for safe, reliable navigation. This paper proposes a new method for sub-pixel stereo disparity estimation, based on ideas from Lucas-Kanade tracking and optical flow, which substantially reduces the pixel-locking effect. In addition, it has the ability to correct much larger initial disparity errors than previous approaches and is more general as it applies not only to the ground plane.

The study of stereo vision technique for the autonomous vehicle

NASA Astrophysics Data System (ADS)

Li, Pei; Wang, Xi; Wang, Jiang-feng

2015-08-01

The stereo vision technology by two or more cameras could recovery 3D information of the field of view. This technology can effectively help the autonomous navigation system of unmanned vehicle to judge the pavement conditions within the field of view, and to measure the obstacles on the road. In this paper, the stereo vision technology in measuring the avoidance of the autonomous vehicle is studied and the key techniques are analyzed and discussed. The system hardware of the system is built and the software is debugged, and finally the measurement effect is explained by the measured data. Experiments show that the 3D reconstruction, within the field of view, can be rebuilt by the stereo vision technology effectively, and provide the basis for pavement condition judgment. Compared with unmanned vehicle navigation radar used in measuring system, the stereo vision system has the advantages of low cost, distance and so on, it has a good application prospect.

STEREO as a "Planetary Hazards" Mission

NASA Technical Reports Server (NTRS)

Guhathakurta, M.; Thompson, B. J.

2014-01-01

NASA's twin STEREO probes, launched in 2006, have advanced the art and science of space weather forecasting more than any other spacecraft or solar observatory. By surrounding the Sun, they provide previously-impossible early warnings of threats approaching Earth as they develop on the solar far side. They have also revealed the 3D shape and inner structure of CMEs-massive solar storms that can trigger geomagnetic storms when they collide with Earth. This improves the ability of forecasters to anticipate the timing and severity of such events. Moreover, the unique capability of STEREO to track CMEs in three dimensions allows forecasters to make predictions for other planets, giving rise to the possibility of interplanetary space weather forecasting too. STEREO is one of those rare missions for which "planetary hazards" refers to more than one world. The STEREO probes also hold promise for the study of comets and potentially hazardous asteroids.

KSC-06pd2261

NASA Image and Video Library

2006-10-10

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the STEREO spacecraft is being moved out of the high bay. A truck will transport the spacecraft to Launch Pad 17-B on Cape Canaveral Air Force Station where it will be lifted into the mobile service tower. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2261a

NASA Image and Video Library

2006-10-10

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the transporter carrying the STEREO spacecraft is secured to the truck that will transport it to Launch Pad 17-B on Cape Canaveral Air Force Station. At the pad, the spacecraft will be lifted into the mobile service tower. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2262

NASA Image and Video Library

2006-10-10

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the transporter carrying the STEREO spacecraft is attached to the truck for transportation to Launch Pad 17-B on Cape Canaveral Air Force Station. At the pad the spacecraft will be lifted into the mobile service tower. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard Space Flight Center. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. STEREO is expected to lift off Oct. 25. Photo credit: NASA/George Shelton

KSC-06pd2277

NASA Image and Video Library

2006-10-11

KENNEDY SPACE CENTER, FLA. - Inside the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, workers help guide the upper segement of the transportation canister away from the STEREO spacecraft. STEREO is being prepared for launch, scheduled for Oct. 25. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft that will launch in a piggyback mode, separating after reaching the appropriate orbit. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. The STEREO mission is managed by Goddard. The Applied Physics Laboratory designed and built the spacecraft. The laboratory will maintain command and control of the observatories throughout the mission, while NASA tracks and receives the data, determines the orbit of the satellites, and coordinates the science results. Photo credit: NASA/Jim Grossmann

Research on three-dimensional reconstruction method based on binocular vision

NASA Astrophysics Data System (ADS)

Li, Jinlin; Wang, Zhihui; Wang, Minjun

2018-03-01

As the hot and difficult issue in computer vision, binocular stereo vision is an important form of computer vision,which has a broad application prospects in many computer vision fields,such as aerial mapping,vision navigation,motion analysis and industrial inspection etc.In this paper, a research is done into binocular stereo camera calibration, image feature extraction and stereo matching. In the binocular stereo camera calibration module, the internal parameters of a single camera are obtained by using the checkerboard lattice of zhang zhengyou the field of image feature extraction and stereo matching, adopted the SURF operator in the local feature operator and the SGBM algorithm in the global matching algorithm are used respectively, and the performance are compared. After completed the feature points matching, we can build the corresponding between matching points and the 3D object points using the camera parameters which are calibrated, which means the 3D information.

Application of Stereo Vision to the Reconnection Scaling Experiment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klarenbeek, Johnny; Sears, Jason A.; Gao, Kevin W.

The measurement and simulation of the three-dimensional structure of magnetic reconnection in astrophysical and lab plasmas is a challenging problem. At Los Alamos National Laboratory we use the Reconnection Scaling Experiment (RSX) to model 3D magnetohydrodynamic (MHD) relaxation of plasma filled tubes. These magnetic flux tubes are called flux ropes. In RSX, the 3D structure of the flux ropes is explored with insertable probes. Stereo triangulation can be used to compute the 3D position of a probe from point correspondences in images from two calibrated cameras. While common applications of stereo triangulation include 3D scene reconstruction and robotics navigation, wemore » will investigate the novel application of stereo triangulation in plasma physics to aid reconstruction of 3D data for RSX plasmas. Several challenges will be explored and addressed, such as minimizing 3D reconstruction errors in stereo camera systems and dealing with point correspondence problems.« less

Use of scanning electron microscopy and microanalysis to determine chloride content of concrete and raw materials.

DOT National Transportation Integrated Search

2013-02-01

Standard sample sets of cement and mortar formulations with known levels of Cl as well as concrete samples subject to Cl diffusion were all prepared for and analyzed with scanning electron microscopy (SEM) and electron microprobe (EPMA). Using x-ray ...

Comparative morphology of zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel sperm: Light and electron microscopy

USGS Publications Warehouse

Walker, G.K.; Black, M.G.; Edwards, C.A.

1996-01-01

Adult zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussels were induced to release large quantities of live spermatozoa by the administration of 5-hydroxytryptamine (serotonin). Sperm were photographed alive using phase-contrast microscopy and were fixed subsequently with glutaraldehyde followed by osmium tetroxide for eventual examination by transmission or scanning electron microscopy. The sperm of both genera are of the ect-aquasperm type. Their overall dimensions and shape allow for easy discrimination at the light and scanning electron microscopy level. Transmission electron microscopy of the cells reveals a barrel-shaped nucleus in zebra mussel sperm and an elongated nucleus in quagga mussel sperm. In both species, an acrosome is cradled in a nuclear fossa. The ultrastructure of the acrosome and axial body, however, is distinctive for each species. The structures of the midpiece are shown, including a unique mitochondrial "skirt" that includes densely packed parallel cristae and extends in a narrow sheet from the mitochondria.

Advances in high-resolution imaging--techniques for three-dimensional imaging of cellular structures.

PubMed

Lidke, Diane S; Lidke, Keith A

2012-06-01

A fundamental goal in biology is to determine how cellular organization is coupled to function. To achieve this goal, a better understanding of organelle composition and structure is needed. Although visualization of cellular organelles using fluorescence or electron microscopy (EM) has become a common tool for the cell biologist, recent advances are providing a clearer picture of the cell than ever before. In particular, advanced light-microscopy techniques are achieving resolutions below the diffraction limit and EM tomography provides high-resolution three-dimensional (3D) images of cellular structures. The ability to perform both fluorescence and electron microscopy on the same sample (correlative light and electron microscopy, CLEM) makes it possible to identify where a fluorescently labeled protein is located with respect to organelle structures visualized by EM. Here, we review the current state of the art in 3D biological imaging techniques with a focus on recent advances in electron microscopy and fluorescence super-resolution techniques.

Microchemical Analysis Of Space Operation Debris

NASA Technical Reports Server (NTRS)

Cummings, Virginia J.; Kim, Hae Soo

1995-01-01

Report discusses techniques used in analyzing debris relative to space shuttle operations. Debris collected from space shuttle, expendable launch vehicles, payloads carried by space shuttle, and payloads carried by expendable launch vehicles. Optical microscopy, scanning electron microscopy with energy-dispersive spectrometry, analytical electron microscopy with wavelength-dispersive spectrometry, and X-ray diffraction chosen as techniques used in examining samples of debris.

Publications - GMC 357 | Alaska Division of Geological & Geophysical

Science.gov Websites

DGGS GMC 357 Publication Details Title: Thin Section and Scanning Electron Microscopy summary Laboratories, Inc., 2008, Thin Section and Scanning Electron Microscopy summary photographs from plugs taken

Electron microscopy of electromagnetic waveforms.

PubMed

Ryabov, A; Baum, P

2016-07-22

Rapidly changing electromagnetic fields are the basis of almost any photonic or electronic device operation. We report how electron microscopy can measure collective carrier motion and fields with subcycle and subwavelength resolution. A collimated beam of femtosecond electron pulses passes through a metamaterial resonator that is previously excited with a single-cycle electromagnetic pulse. If the probing electrons are shorter in duration than half a field cycle, then time-frozen Lorentz forces distort the images quasi-classically and with subcycle time resolution. A pump-probe sequence reveals in a movie the sample's oscillating electromagnetic field vectors with time, phase, amplitude, and polarization information. This waveform electron microscopy can be used to visualize electrodynamic phenomena in devices as small and fast as available. Copyright © 2016, American Association for the Advancement of Science.

Murphy's law-if anything can go wrong, it will: Problems in phage electron microscopy.

PubMed

Ackermann, Hans-W; Tiekotter, Kenneth L

2012-04-01

The quality of bacteriophage electron microscopy appears to be on a downward course since the 1980s. This coincides with the introduction of digital electron microscopes and a general lowering of standards, possibly due to the disappearance of several world-class electron microscopists The most important problem seems to be poor contrast. Positive staining is frequently not recognized as an undesirable artifact. Phage parts, bacterial debris, and aberrant or damaged phage particles may be misdiagnosed as bacterial viruses. Digital electron microscopes often seem to be operated without magnification control because this is difficult and inconvenient. In summary, most phage electron microscopy problems may be attributed to human failure. Journals are a last-ditch defense and have a heavy responsibility in selecting competent reviewers and rejecting, or not, unsatisfactory articles.

Stereo 3-D Vision in Teaching Physics

ERIC Educational Resources Information Center

Zabunov, Svetoslav

2012-01-01

Stereo 3-D vision is a technology used to present images on a flat surface (screen, paper, etc.) and at the same time to create the notion of three-dimensional spatial perception of the viewed scene. A great number of physical processes are much better understood when viewed in stereo 3-D vision compared to standard flat 2-D presentation. The…

Method for Stereo Mapping Based on Objectarx and Pipeline Technology

NASA Astrophysics Data System (ADS)

Liu, F.; Chen, T.; Lin, Z.; Yang, Y.

2012-07-01

Stereo mapping is an important way to acquire 4D production. Based on the development of the stereo mapping and the characteristics of ObjectARX and pipeline technology, a new stereo mapping scheme which can realize the interaction between the AutoCAD and digital photogrammetry system is offered by ObjectARX and pipeline technology. An experiment is made in order to make sure the feasibility with the example of the software MAP-AT (Modern Aerial Photogrammetry Automatic Triangulation), the experimental results show that this scheme is feasible and it has very important meaning for the realization of the acquisition and edit integration.

Reversed stereo depth and motion direction with anti-correlated stimuli.

PubMed

Read, J C; Eagle, R A

2000-01-01

We used anti-correlated stimuli to compare the correspondence problem in stereo and motion. Subjects performed a two-interval forced-choice disparity/motion direction discrimination task for different displacements. For anti-correlated 1d band-pass noise, we found weak reversed depth and motion. With 2d anti-correlated stimuli, stereo performance was impaired, but the perception of reversed motion was enhanced. We can explain the main features of our data in terms of channels tuned to different spatial frequencies and orientation. We suggest that a key difference between the solution of the correspondence problem by the motion and stereo systems concerns the integration of information at different orientations.

Stereo imaging with spaceborne radars

NASA Technical Reports Server (NTRS)

Leberl, F.; Kobrick, M.

1983-01-01

Stereo viewing is a valuable tool in photointerpretation and is used for the quantitative reconstruction of the three dimensional shape of a topographical surface. Stereo viewing refers to a visual perception of space by presenting an overlapping image pair to an observer so that a three dimensional model is formed in the brain. Some of the observer's function is performed by machine correlation of the overlapping images - so called automated stereo correlation. The direct perception of space with two eyes is often called natural binocular vision; techniques of generating three dimensional models of the surface from two sets of monocular image measurements is the topic of stereology.

KSC-06pd1546

NASA Image and Video Library

2006-07-13

KENNEDY SPACE CENTER, FLA. - At Launch Pad 17-B on Cape Canaveral Air Force Station, workers prepare the Boeing Delta II second stage for the STEREO launch to be lifted off the transporter. The second stage then will be lifted into the mobile service tower and mated with first stage already in place. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off in August 2006. Photo credit: NASA/George Shelton

KSC-06pd1868

NASA Image and Video Library

2006-08-11

KENNEDY SPACE CENTER, FLA. - The STEREO observatories are the focus of attention at a media viewing held at Astrotech Space Operations in Titusville, Fla., on Aug. 11. The two observatories were mated for launch but will separate into different orbits for their mission. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1867

NASA Image and Video Library

2006-08-11

KENNEDY SPACE CENTER, FLA. - The STEREO observatories are the focus of attention at a media viewing held at Astrotech Space Operations in Titusville, Fla., on Aug. 11. The two observatories were mated for launch but will separate into different orbits for their mission. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1535

NASA Image and Video Library

2006-07-10

KENNEDY SPACE CENTER, FLA. - In the hazardous processing facility at Astrotech Space Operations in Titusville, Fla., technicians remove the protective cover from the top of Observatory A, one of two STEREO spacecraft. The observatory will be lifted onto a scale for weight measurements and later will be fueled. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket no earlier than Aug. 1. Photo credit: NASA/Jack Pfaller

KSC-06pd1864

NASA Image and Video Library

2006-08-11

KENNEDY SPACE CENTER, FLA. - The STEREO observatories are the focus of attention at a media viewing held at Astrotech Space Operations in Titusville, Fla., on Aug. 11. The two observatories were mated for launch but will separate into different orbits for their mission. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1534

NASA Image and Video Library

2006-07-10

KENNEDY SPACE CENTER, FLA. - In the hazardous processing facility at Astrotech Space Operations in Titusville, Fla., technicians begin removing the protective cover from Observatory A of the STEREO spacecraft. The observatory will be lifted onto a scale for weight measurements and later will be fueled. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket no earlier than Aug. 1. Photo credit: NASA/Jack Pfaller

KSC-06pd1862

NASA Image and Video Library

2006-08-11

KENNEDY SPACE CENTER, FLA. - The STEREO observatories are the focus of attention at a media viewing held at Astrotech Space Operations in Titusville, Fla. The two observatories were mated for launch but will separate into different orbits for their mission. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1533

NASA Image and Video Library

2006-07-10

KENNEDY SPACE CENTER, FLA. - In the hazardous processing facility at Astrotech Space Operations in Titusville, Fla., technicians begin removing the protective cover from Observatory A of the STEREO spacecraft. The observatory will be lifted onto a scale for weight measurements and later will be fueled. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket no earlier than Aug. 1. Photo credit: NASA/Jack Pfaller

KSC-06pd1860

NASA Image and Video Library

2006-08-11

KENNEDY SPACE CENTER, FLA. - The STEREO observatories are the focus of attention at a media viewing held at Astrotech Space Operations in Titusville, Fla. The two observatories were mated for launch but will separate into different orbits for their mission. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1885

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., workers check the progress of the upper portion of the transportation canister as it is lowered over the STEREO spacecraft. When the entire configuration is encased, it will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

KSC-06pd1863

NASA Image and Video Library

2006-08-11

KENNEDY SPACE CENTER, FLA. - The STEREO observatories are the focus of attention at a media viewing held at Astrotech Space Operations in Titusville, Fla. The two observatories were mated for launch but will separate into different orbits for their mission. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1873

NASA Image and Video Library

2006-08-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., workers begin mating the STEREO spacecraft onto the upper stage booster below. After the mating, they will be transported to Launch Pad 17-B at Cape Canaveral Air Force Station for integration with the Delta II already on the pad. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/George Shelton

KSC-06pd1884

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., a worker examines the STEREO spacecraft as the upper portion of the transportation canister is lowered over the top. When the entire configuration is encased, it will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

KSC-06pd1540

NASA Image and Video Library

2006-07-10

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., technicians perform black-light inspection and cleaning of Observatory B, part of the STEREO spacecraft. The observatory will later be wrapped for transfer to the hazardous processing facility where it will be weighed and fueled. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket no earlier than Aug. 1. Photo credit: NASA/George Shelton

KSC-06pd1549

NASA Image and Video Library

2006-07-13

KENNEDY SPACE CENTER, FLA. - At Launch Pad 17-B on Cape Canaveral Air Force Station, the Boeing Delta II second stage for the STEREO launch is moved inside the mobile service tower. The second stage then will be lifted into the mobile service tower and mated with first stage already in place. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off in August 2006. Photo credit: NASA/George Shelton

KSC-06pd1866

NASA Image and Video Library

2006-08-11

KENNEDY SPACE CENTER, FLA. - The STEREO observatories are the focus of attention at a media viewing held at Astrotech Space Operations in Titusville, Fla., on Aug. 11. The two observatories were mated for launch but will separate into different orbits for their mission. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off on Aug. 31, from Launch Pad 17-B on Cape Canaveral Air Force Station in Florida. Photo credit: NASA/George Shelton.

KSC-06pd1545

NASA Image and Video Library

2006-07-13

KENNEDY SPACE CENTER, FLA. - At Launch Pad 17-B on Cape Canaveral Air Force Station, the Boeing Delta II second stage for the STEREO launch arrives on a transporter. The second stage will be lifted into the mobile service tower and mated with the first stage already in place. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off in August 2006. Photo credit: NASA/George Shelton

KSC-06pd1881

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., workers maneuver the upper portion of the transportation canister that will be lowered over the STEREO spacecraft (in front). When the entire configuration is encased, it will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

KSC-06pd1879

NASA Image and Video Library

2006-08-18

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., workers begin to install the lower portion of the transportation canister around the upper stage booster attached to the STEREO spacecraft (above). The entire configuration will be encased for the move to Launch Pad 17-B at Cape Canaveral Air Force Station. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/Kim Shiflett

A study on low-cost, high-accuracy, and real-time stereo vision algorithms for UAV power line inspection

NASA Astrophysics Data System (ADS)

Wang, Hongyu; Zhang, Baomin; Zhao, Xun; Li, Cong; Lu, Cunyue

2018-04-01

Conventional stereo vision algorithms suffer from high levels of hardware resource utilization due to algorithm complexity, or poor levels of accuracy caused by inadequacies in the matching algorithm. To address these issues, we have proposed a stereo range-finding technique that produces an excellent balance between cost, matching accuracy and real-time performance, for power line inspection using UAV. This was achieved through the introduction of a special image preprocessing algorithm and a weighted local stereo matching algorithm, as well as the design of a corresponding hardware architecture. Stereo vision systems based on this technique have a lower level of resource usage and also a higher level of matching accuracy following hardware acceleration. To validate the effectiveness of our technique, a stereo vision system based on our improved algorithms were implemented using the Spartan 6 FPGA. In comparative experiments, it was shown that the system using the improved algorithms outperformed the system based on the unimproved algorithms, in terms of resource utilization and matching accuracy. In particular, Block RAM usage was reduced by 19%, and the improved system was also able to output range-finding data in real time.

Stereopsis cueing effects on hover-in-turbulence performance in a simulated rotorcraft

NASA Technical Reports Server (NTRS)

Parrish, Russell V.; Williams, Steven P.

1990-01-01

The efficacy of stereopsis cueing in pictorial displays was assessed in a real-time piloted simulation experiment of a rotorcraft precision hover-in-turbulence task. Seven pilots endeavored to maintain a hover by visually aligning a set of inner and outer wickets (major elements of a real-world pictorial display, thus attaining the desired hover position, in a full factorial experimental design. The display conditions examined included the presence or absence of a velocity display element (a velocity head-up display) as well as the stereopsis cueing conditions, which included non-stereo (binoptic or monoscopic - no depth cues other than those provided by a perspective, real-world display), stereo 3-D, and hyper stereo (telestereoscopic). Subjective and objective results indicated that the depth cues provided by the stereo displays enhanced the situational awareness of the pilot and enabled improved hover performance to be achieved. The velocity display element also improved the hover performance, with the best hover performance being achieved with the combined use of stereo and the velocity display element. Pilot control input data revealed that less control action was required to attain the improved hover performance with the stereo displays.

Research on the feature set construction method for spherical stereo vision

NASA Astrophysics Data System (ADS)

Zhu, Junchao; Wan, Li; Röning, Juha; Feng, Weijia

2015-01-01

Spherical stereo vision is a kind of stereo vision system built by fish-eye lenses, which discussing the stereo algorithms conform to the spherical model. Epipolar geometry is the theory which describes the relationship of the two imaging plane in cameras for the stereo vision system based on perspective projection model. However, the epipolar in uncorrected fish-eye image will not be a line but an arc which intersects at the poles. It is polar curve. In this paper, the theory of nonlinear epipolar geometry will be explored and the method of nonlinear epipolar rectification will be proposed to eliminate the vertical parallax between two fish-eye images. Maximally Stable Extremal Region (MSER) utilizes grayscale as independent variables, and uses the local extremum of the area variation as the testing results. It is demonstrated in literatures that MSER is only depending on the gray variations of images, and not relating with local structural characteristics and resolution of image. Here, MSER will be combined with the nonlinear epipolar rectification method proposed in this paper. The intersection of the rectified epipolar and the corresponding MSER region is determined as the feature set of spherical stereo vision. Experiments show that this study achieved the expected results.

Congruence analysis of point clouds from unstable stereo image sequences

NASA Astrophysics Data System (ADS)

Jepping, C.; Bethmann, F.; Luhmann, T.

2014-06-01

This paper deals with the correction of exterior orientation parameters of stereo image sequences over deformed free-form surfaces without control points. Such imaging situation can occur, for example, during photogrammetric car crash test recordings where onboard high-speed stereo cameras are used to measure 3D surfaces. As a result of such measurements 3D point clouds of deformed surfaces are generated for a complete stereo sequence. The first objective of this research focusses on the development and investigation of methods for the detection of corresponding spatial and temporal tie points within the stereo image sequences (by stereo image matching and 3D point tracking) that are robust enough for a reliable handling of occlusions and other disturbances that may occur. The second objective of this research is the analysis of object deformations in order to detect stable areas (congruence analysis). For this purpose a RANSAC-based method for congruence analysis has been developed. This process is based on the sequential transformation of randomly selected point groups from one epoch to another by using a 3D similarity transformation. The paper gives a detailed description of the congruence analysis. The approach has been tested successfully on synthetic and real image data.

A stereo remote sensing feature selection method based on artificial bee colony algorithm

NASA Astrophysics Data System (ADS)

Yan, Yiming; Liu, Pigang; Zhang, Ye; Su, Nan; Tian, Shu; Gao, Fengjiao; Shen, Yi

2014-05-01

To improve the efficiency of stereo information for remote sensing classification, a stereo remote sensing feature selection method is proposed in this paper presents, which is based on artificial bee colony algorithm. Remote sensing stereo information could be described by digital surface model (DSM) and optical image, which contain information of the three-dimensional structure and optical characteristics, respectively. Firstly, three-dimensional structure characteristic could be analyzed by 3D-Zernike descriptors (3DZD). However, different parameters of 3DZD could descript different complexity of three-dimensional structure, and it needs to be better optimized selected for various objects on the ground. Secondly, features for representing optical characteristic also need to be optimized. If not properly handled, when a stereo feature vector composed of 3DZD and image features, that would be a lot of redundant information, and the redundant information may not improve the classification accuracy, even cause adverse effects. To reduce information redundancy while maintaining or improving the classification accuracy, an optimized frame for this stereo feature selection problem is created, and artificial bee colony algorithm is introduced for solving this optimization problem. Experimental results show that the proposed method can effectively improve the computational efficiency, improve the classification accuracy.

[Research on output and quality of Panax notoginseng and annual change characteristics of N, P and K nutrients of planting soil under stereo-cultivation].

PubMed

Huang, Chun-mei; Cui, Xiu-ming; Lan, Lei; Chen, Wei-dong; Wang, Cheng-xiao; Yang, Xiao-yan; Lu, Da-hui; Yang, Ye

2015-08-01

The output and agronomic characters of 3-year-old Panax notoginseng cultured under stereo structure (upper, middle and down layers) were investigated, and the annual change of N, P and K of its planting soil were also studied. Results showed that, compared with field cultured Panax notoginseng, growth vigour and output of stereo-cultivation were significantly lower. But the total production of the 3 layers was 1.6 times of field. The growth vigor and production of P. notoginseng was in the order of upper layer > middle layer > down layer. The content of ginsenoside in rhizome, root tuber and hair root of P. notoginseng was in the order of upper layer > field > middle layer > down layer. Organic matter content and pH of stereo-cultivation soil decreased with the prolonging of planting time, which with the same trend of yield. Organic matter content of stereo-cultivation soil was significantly higher than field, but the pH was significantly lower. Contents of total and available N, P and K in stereo-cultivation soil and field decreased with the prolonging of planting time. The content of N and P were in the order of upper layer > middle layer > yield > down layer, the content of K was in the order of upper layer > middle layer > down layer > yield. Compared with field, the proportion of N and P in the organ of underground (rhizome, root tuber and hair root) of upper layer were increased, while decreased in middle and down layers. Proportion of K in underground decreased significantly of the 3 layers. In conclusion, the agronomic characters and production of stereo-cultivation were significantly lower than that of yield. But the total production of the 3 layers were significantly higher than field of unit area. And the aim of improving land utilization efficiency was achieved. Nutritions in the soil of stereo-cultivation were enough to support the development of P. notoginseng, which was not the cause of weak growth and low production. The absorbing ability of P. notoginseng to N, P and K nutrients was decreased by stereo-cultivation mode. So, improve the growth vigour of P. notoginseng from the perspective of adjusting the stereo-cultivation mode so as to improve the nutrient absorption capacity is the future direction.

TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria

DOE Office of Scientific and Technical Information (OSTI.GOV)

Alba, Martha P.; Suarez, Carlos F.; Universidad del Rosario, Bogotá D. C.

Fully-protective, long-lasting, immunological (FPLLI) memory against Plasmodium falciparum malaria regarding immune protection-inducing protein structures (IMPIPS) vaccinated into monkeys previously challenged and re-challenged 60 days later with a lethal Aotus monkey-adapted P. falciparum strain was found to be associated with preferential high binding capacity to HLA-DRβ1* allelic molecules of the major histocompatibility class II (MHC-II), rather than HLA-DRβ3*, β4*, β5* alleles. Complete PPII{sub L} 3D structure, a longer distance (26.5 Å ± 1.5 Å) between residues perfectly fitting into HLA-DRβ1*PBR pockets 1 and 9, a gauche{sup −} rotamer orientation in p8 TCR-contacting polar residue and a larger volume of polar p2 residues was also found. Thismore » data, in association with previously-described p3 and p7 apolar residues having gauche{sup +} orientation to form a perfect MHC-II-peptide-TCR complex, determines the stereo-electronic and topochemical characteristics associated with FPLLI immunological memory. - Highlights: • Stereo-electronic and topochemical rules associated with FPLLI immunological memory. • Presence of very high long-lasting antibody titres against Plasmodium falciparum Spz. • Protective memory induction associated with a binding capacity to HLA-DRβ1*. • gauche{sup −} rotamer orientation in p8 polar residue is related to is related to immunological memory.« less

THE THOMSON SURFACE. I. REALITY AND MYTH

DOE Office of Scientific and Technical Information (OSTI.GOV)

Howard, T. A.; DeForest, C. E., E-mail: howard@boulder.swri.edu

2012-06-20

The solar corona and heliosphere are visible via sunlight that is Thomson-scattered off free electrons and detected by coronagraphs and heliospheric imagers. It is well known that these instruments are most responsive to material at the 'Thomson surface', the sphere with a diameter passing through both the observer and the Sun. It is less well known that in fact the Thomson scattering efficiency is minimized on the Thomson surface. Unpolarized heliospheric imagers such as STEREO/HI are thus approximately equally responsive to material over more than a 90 Degree-Sign range of solar exit angles at each given position in the imagemore » plane. We call this range of angles the 'Thomson plateau'. We observe that heliospheric imagers are actually more sensitive to material far from the Thomson surface than close to it, at a fixed radius from the Sun. We review the theory of Thomson scattering as applied to heliospheric imaging, feature detection in the presence of background noise, geometry inference, and feature mass measurement. We show that feature detection is primarily limited by observing geometry and field of view, that the highest sensitivity for detection of density features is to objects close to the observer, that electron surface density inference is independent of geometry across the Thomson plateau, and that mass inference varies with observer distance in all geometries. We demonstrate the sensitivity results with a few examples of features detected by STEREO, far from the Thomson surface.« less

75 FR 34096 - Application(s) for Duty-Free Entry of Scientific Instruments

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-16

... dynamin, using negative stain nad cryo-electron microscopy methods. Justification for Duty-Free Entry..., using negative stain nad cryo-electron microscopy methods. Justification for Duty-Free Entry: There are...

Understanding materials challenges for rechargeable ion batteries with in situ transmission electron microscopy

NASA Astrophysics Data System (ADS)

Yuan, Yifei; Amine, Khalil; Lu, Jun; Shahbazian-Yassar, Reza

2017-08-01

An in-depth understanding of material behaviours under complex electrochemical environment is critical for the development of advanced materials for the next-generation rechargeable ion batteries. The dynamic conditions inside a working battery had not been intensively explored until the advent of various in situ characterization techniques. Real-time transmission electron microscopy of electrochemical reactions is one of the most significant breakthroughs poised to enable radical shift in our knowledge on how materials behave in the electrochemical environment. This review, therefore, summarizes the scientific discoveries enabled by in situ transmission electron microscopy, and specifically emphasizes the applicability of this technique to address the critical challenges in the rechargeable ion battery electrodes, electrolyte and their interfaces. New electrochemical systems such as lithium-oxygen, lithium-sulfur and sodium ion batteries are included, considering the rapidly increasing application of in situ transmission electron microscopy in these areas. A systematic comparison between lithium ion-based electrochemistry and sodium ion-based electrochemistry is also given in terms of their thermodynamic and kinetic differences. The effect of the electron beam on the validity of in situ observation is also covered. This review concludes by providing a renewed perspective for the future directions of in situ transmission electron microscopy in rechargeable ion batteries.

Understanding materials challenges for rechargeable ion batteries with in situ transmission electron microscopy

PubMed Central

Yuan, Yifei; Amine, Khalil; Lu, Jun; Shahbazian-Yassar, Reza

2017-01-01

An in-depth understanding of material behaviours under complex electrochemical environment is critical for the development of advanced materials for the next-generation rechargeable ion batteries. The dynamic conditions inside a working battery had not been intensively explored until the advent of various in situ characterization techniques. Real-time transmission electron microscopy of electrochemical reactions is one of the most significant breakthroughs poised to enable radical shift in our knowledge on how materials behave in the electrochemical environment. This review, therefore, summarizes the scientific discoveries enabled by in situ transmission electron microscopy, and specifically emphasizes the applicability of this technique to address the critical challenges in the rechargeable ion battery electrodes, electrolyte and their interfaces. New electrochemical systems such as lithium–oxygen, lithium–sulfur and sodium ion batteries are included, considering the rapidly increasing application of in situ transmission electron microscopy in these areas. A systematic comparison between lithium ion-based electrochemistry and sodium ion-based electrochemistry is also given in terms of their thermodynamic and kinetic differences. The effect of the electron beam on the validity of in situ observation is also covered. This review concludes by providing a renewed perspective for the future directions of in situ transmission electron microscopy in rechargeable ion batteries.

The Electron Microscopy Outreach Program: A Web-based resource for research and education.

PubMed

Sosinsky, G E; Baker, T S; Hand, G; Ellisman, M H

1999-01-01

We have developed a centralized World Wide Web (WWW)-based environment that serves as a resource of software tools and expertise for biological electron microscopy. A major focus is molecular electron microscopy, but the site also includes information and links on structural biology at all levels of resolution. This site serves to help integrate or link structural biology techniques in accordance with user needs. The WWW site, called the Electron Microscopy (EM) Outreach Program (URL: http://emoutreach.sdsc.edu), provides scientists with computational and educational tools for their research and edification. In particular, we have set up a centralized resource containing course notes, references, and links to image analysis and three-dimensional reconstruction software for investigators wanting to learn about EM techniques either within or outside of their fields of expertise. Copyright 1999 Academic Press.

A compilation of cold cases using scanning electron microscopy at the University of Rhode Island

NASA Astrophysics Data System (ADS)

Platek, Michael J.; Gregory, Otto J.

2015-10-01

Scanning electron microscopy combined with microchemical analysis has evolved into one of the most widely used instruments in forensic science today. In particular, the environmental scanning electron microscope (SEM) in conjunction with energy dispersive spectroscopy (EDS), has created unique opportunities in forensic science in regard to the examination of trace evidence; i.e. the examination of evidence without altering the evidence with conductive coatings, thereby enabling criminalists to solve cases that were previously considered unsolvable. Two cold cases were solved at URI using a JEOL 5900 LV SEM in conjunction with EDS. A cold case murder and a cold missing person case will be presented from the viewpoint of the microscopist and will include sample preparation, as well as image and chemical analysis of the trace evidence using electron microscopy and optical microscopy.

The EIGER detector for low-energy electron microscopy and photoemission electron microscopy.

PubMed

Tinti, G; Marchetto, H; Vaz, C A F; Kleibert, A; Andrä, M; Barten, R; Bergamaschi, A; Brückner, M; Cartier, S; Dinapoli, R; Franz, T; Fröjdh, E; Greiffenberg, D; Lopez-Cuenca, C; Mezza, D; Mozzanica, A; Nolting, F; Ramilli, M; Redford, S; Ruat, M; Ruder, Ch; Schädler, L; Schmidt, Th; Schmitt, B; Schütz, F; Shi, X; Thattil, D; Vetter, S; Zhang, J

2017-09-01

EIGER is a single-photon-counting hybrid pixel detector developed at the Paul Scherrer Institut, Switzerland. It is designed for applications at synchrotron light sources with photon energies above 5 keV. Features of EIGER include a small pixel size (75 µm × 75 µm), a high frame rate (up to 23 kHz), a small dead-time between frames (down to 3 µs) and a dynamic range up to 32-bit. In this article, the use of EIGER as a detector for electrons in low-energy electron microscopy (LEEM) and photoemission electron microscopy (PEEM) is reported. It is demonstrated that, with only a minimal modification to the sensitive part of the detector, EIGER is able to detect electrons emitted or reflected by the sample and accelerated to 8-20 keV. The imaging capabilities are shown to be superior to the standard microchannel plate detector for these types of applications. This is due to the much higher signal-to-noise ratio, better homogeneity and improved dynamic range. In addition, the operation of the EIGER detector is not affected by radiation damage from electrons in the present energy range and guarantees more stable performance over time. To benchmark the detector capabilities, LEEM experiments are performed on selected surfaces and the magnetic and electronic properties of individual iron nanoparticles with sizes ranging from 8 to 22 nm are detected using the PEEM endstation at the Surface/Interface Microscopy (SIM) beamline of the Swiss Light Source.

Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film.

PubMed

Nishiyama, Hidetoshi; Suga, Mitsuo; Ogura, Toshihiko; Maruyama, Yuusuke; Koizumi, Mitsuru; Mio, Kazuhiro; Kitamura, Shinichi; Sato, Chikara

2010-03-01

Direct observation of subcellular structures and their characterization is essential for understanding their physiological functions. To observe them in open environment, we have developed an inverted scanning electron microscope with a detachable, open-culture dish, capable of 8 nm resolution, and combined with a fluorescence microscope quasi-simultaneously observing the same area from the top. For scanning electron microscopy from the bottom, a silicon nitride film window in the base of the dish maintains a vacuum between electron gun and open sample dish while allowing electrons to pass through. Electrons are backscattered from the sample and captured by a detector under the dish. Cells cultured on the open dish can be externally manipulated under optical microscopy, fixed, and observed using scanning electron microscopy. Once fine structures have been revealed by scanning electron microscopy, their component proteins may be identified by comparison with separately prepared fluorescence-labeled optical microscopic images of the candidate proteins, with their heavy-metal-labeled or stained ASEM images. Furthermore, cell nuclei in a tissue block stained with platinum-blue were successfully observed without thin-sectioning, which suggests the applicability of this inverted scanning electron microscope to cancer diagnosis. This microscope visualizes mesoscopic-scale structures, and is also applicable to non-bioscience fields including polymer chemistry. (c) 2010 Elsevier Inc. All rights reserved.

Room temperature chemical synthesis of lead selenide thin films with preferred orientation

NASA Astrophysics Data System (ADS)

Kale, R. B.; Sartale, S. D.; Ganesan, V.; Lokhande, C. D.; Lin, Yi-Feng; Lu, Shih-Yuan

2006-11-01

Room temperature chemical synthesis of PbSe thin films was carried out from aqueous ammoniacal solution using Pb(CH3COO)2 as Pb2+ and Na2SeSO3 as Se2- ion sources. The films were characterized by a various techniques including, X-ray diffraction (XRD), energy dispersive X-ray analysis (EDAX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high resolution transmission electron microscopy (HR-TEM), selected area electron diffraction (SAED), Fast Fourier transform (FFT) and UV-vis-NIR techniques. The study revealed that the PbSe thin film consists of preferentially oriented nanocubes with energy band gap of 0.5 eV.

Imaging of high-angle annular dark-field scanning transmission electron microscopy and observations of GaN-based violet laser diodes.

PubMed

Shiojiri, M; Saijo, H

2006-09-01

The first part of this paper is devoted to physics, to explain high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) imaging and to interpret why HAADF-STEM imaging is incoherent, instructing a strict definition of interference and coherence of electron waves. Next, we present our recent investigations of InGaN/GaN multiple quantum wells and AlGaN/GaN strained-layer superlattice claddings in GaN-based violet laser diodes, which have been performed by HAADF-STEM and high-resolution field-emission gun scanning electron microscopy.

Application of He ion microscopy for material analysis

NASA Astrophysics Data System (ADS)

Altmann, F.; Simon, M.; Klengel, R.

2009-05-01

Helium ion beam microscopy (HIM) is a new high resolution imaging technique. The use of Helium ions instead of electrons enables none destructive imaging combined with contrasts quite similar to that from Gallium ion beam imaging. The use of very low probe currents and the comfortable charge compensation using low energy electrons offer imaging of none conductive samples without conductive coating. An ongoing microelectronic sample with Gold/Aluminum interconnects and polymer electronic devices were chosen to evaluate HIM in comparison to scanning electron microscopy (SEM). The aim was to look for key applications of HIM in material analysis. Main focus was on complementary contrast mechanisms and imaging of none conductive samples.

Catalytic chemical vapor deposition synthesis and electron microscopy observation of coiled carbon nanotubes

NASA Astrophysics Data System (ADS)

Xie, Jining; Mukhopadyay, K.; Yadev, J.; Varadan, V. K.

2003-10-01

Coiled carbon nanotubes exhibit excellent mechanical and electrical properties because of the combination of coil morphology and properties of nanotubes. They could have potential novel applications in nanocomposites and nano-electronic devices as well as nano-electromechanical systems. In this work, synthesis of regularly coiled carbon nanotubes is presented. It involves pyrolysis of hydrocarbon gas over metal/support catalyst by both thermal filament and microwave catalytic chemical vapor deposition methods. Scanning electron microscopy and transmission electron microscopy were performed to observe the coil morphology and nanostructure of coiled nanotubes. The growth mechanism and structural and electrical properties of coiled carbon nanotubes are also discussed.

Use of light, scanning electron microscopy and bioassays to evaluate parasitism by entomopathogenic fungi of the red scale insect of palms (Phoenicococcus marlatti Ckll., 1899).

PubMed

Asensio, L; Lopez-Llorca, L V; López-Jiménez, J A

2005-01-01

We have evaluated the parasitism of the red scale insect of the date palm (Phoenicococcus marlatti) by entomopathogenic fungi, using light microscopy (LM), scanning electron microscopy (SEM) and low temperature scanning electron microscopy (LTSEM). Beauveria bassiana, Lecanicillium dimorphum and Lecanicillium cf. psalliotae, were inoculated directly on the scale insects or on insect infested plant material. We found that L. dimorphum and L. cf. psalliotae developed on plant material and on scale insects, making infection structures. B. bassiana was a bad colonizer of date palm leaves (Phoenix dactylifera L.) and did not parasite the scale insects.

Airborne asbestos in Colorado public schools.

PubMed

Chadwick, D A; Buchan, R M; Beaulieu, H J

1985-02-01

Levels of airborne asbestos for six Colorado public school facilities with sprayed-on asbestos materials were documented using three analytical techniques. Phase contrast microscopy showed levels up to the thousandths of a fiber per cubic centimeter (f/cc), scanning electron microscopy (SEM) up to the hundredths of a f/cc, and transmission electron microscopy coupled to selected area electron diffraction and energy dispersive X-ray analysis (TEM-SAED-EDXA) up to the tenths of an asbestos f/cc. Phase contrast microscopy was found to be an inadequate analytical technique for documenting the levels of airborne asbestos fibers in the schools: only large fibers which were not embedded in the filter were counted, and asbestos fibers were not distinguished from nonasbestos.

Further description of Cruzia tentaculata (Rudolphi, 1819) Travassos, 1917 (Nematoda: Cruzidae) by light and scanning electron microscopy.

PubMed

Adnet, F A O; Anjos, D H S; Menezes-Oliveira, A; Lanfredi, R M

2009-04-01

Species of Cruzia are parasites of the large intestine of marsupials, reptiles, amphibians, and mammalians. Cruzia tentaculata specimens were collected from the large intestine of Didelphis marsupialis (Mammalia: Didelphidae) from Colombia (new geographical record) and from Brazil and analyzed by light and scanning electron microscopy. The morphology of males and females by light microscopy corroborated most of the previous description and the ultrastructure by scanning electron microscopy evidence: the topography of the cuticle, deirids, amphids, phasmids in both sexes, a pair of papillae near the vulva opening, and the number and location of male caudal papillae, adding new features for species identification only observed by this technique.

Transparent volume imaging

NASA Astrophysics Data System (ADS)

Wixson, Steve E.

1990-07-01

Transparent Volume Imaging began with the stereo xray in 1895 and ended for most investigators when radiation safety concerns eliminated the second view. Today, similiar images can be generated by the computer without safety hazards providing improved perception and new means of image quantification. A volumetric workstation is under development based on an operational prototype. The workstation consists of multiple symbolic and numeric processors, binocular stereo color display generator with large image memory and liquid crystal shutter, voice input and output, a 3D pointer that uses projection lenses so that structures in 3 space can be touched directly, 3D hard copy using vectograph and lenticular printing, and presentation facilities using stereo 35mm slide and stereo video tape projection. Volumetric software includes a volume window manager, Mayo Clinic's Analyze program and our Digital Stereo Microscope (DSM) algorithms. The DSM uses stereo xray-like projections, rapidly oscillating motion and focal depth cues such that detail can be studied in the spatial context of the entire set of data. Focal depth cues are generated with a lens and apeture algorithm that generates a plane of sharp focus, and multiple stereo pairs each with a different plane of sharp focus are generated and stored in the large memory for interactive selection using a physical or symbolic depth selector. More recent work is studying non-linear focussing. Psychophysical studies are underway to understand how people perce ive images on a volumetric display and how accurately 3 dimensional structures can be quantitated from these displays.

Magnetism of epitaxial Tb films on W(110) studied by spin-polarized low-energy electron microscopy

NASA Astrophysics Data System (ADS)

Prieto, J. E.; Chen, Gong; Schmid, A. K.; de la Figuera, J.

2016-11-01

Thin epitaxial films of Tb metal were grown on a clean W(110) substrate in ultrahigh vacuum and studied in situ by low-energy electron microscopy. Annealed films present magnetic contrast in spin-polarized low-energy electron microscopy. The energy dependence of the electron reflectivity was determined and a maximum value of its spin asymmetry of about 1% was measured. The magnetization direction of the Tb films is in-plane. Upon raising the temperature, no change in the domain distribution is observed, while the asymmetry in the electron reflectivity decreases when approaching the critical temperature, following a power law ˜(1-T /TC) β with a critical exponent β of 0.39.

Feasibility study consisting of a review of contour generation methods from stereograms

NASA Technical Reports Server (NTRS)

Kim, C. J.; Wyant, J. C.

1980-01-01

A review of techniques for obtaining contour information from stereo pairs is given. Photogrammetric principles including a description of stereoscopic vision are presented. The use of conventional contour generation methods, such as the photogrammetric plotting technique, electronic correlator, and digital correlator are described. Coherent optical techniques for contour generation are discussed and compared to the electronic correlator. The optical techniques are divided into two categories: (1) image plane operation and (2) frequency plane operation. The description of image plane correlators are further divided into three categories: (1) image to image correlator, (2) interferometric correlator, and (3) positive negative transparencies. The frequency plane correlators are divided into two categories: (1) correlation of Fourier transforms, and (2) filtering techniques.

Development of Thin Films as Potential Structural Cathodes to Enable Multifunctional Energy-Storage Structural Composite Batteries for the U.S. Army’s Future Force

DTIC Science & Technology

2011-09-01

glancing angle X - ray diffraction (GAXRD), atomic force microscopy (AFM), scanning electron microscopy (SEM), and electrochemical...Emission SEM FWHM full width at half maximum GAXRD glancing angle X - ray diffraction H3COCH2CH2OH 2-methoxyethanol LiMn2O4 lithium manganese oxide...were characterized by scanning electron microscopy (SEM), X - ray diffraction (XRD), and atomic force microscopy (AFM). In addition,

Realistic representation of Bacillus subtilis biofilms architecture using combined microscopy (CLSM, ESEM and FESEM).

PubMed

Bridier, A; Meylheuc, T; Briandet, R

2013-05-01

In this contribution, we used a set of microscopic techniques including confocal laser scanning microscopy (CLSM), environmental scanning electron microscopy (ESEM) and field emission scanning electron microscopy (FESEM) to analyze the three-dimensional spatial arrangement of cells and their surrounding matrix in Bacillus subtilis biofilm. The combination of the different techniques enabled a deeper and realistic deciphering of biofilm architecture by providing the opportunity to overcome the limits of each single technique. Copyright © 2013 Elsevier Ltd. All rights reserved.

Fingerprint-Based Structure Retrieval Using Electron Density

PubMed Central

Yin, Shuangye; Dokholyan, Nikolay V.

2010-01-01

We present a computational approach that can quickly search a large protein structural database to identify structures that fit a given electron density, such as determined by cryo-electron microscopy. We use geometric invariants (fingerprints) constructed using 3D Zernike moments to describe the electron density, and reduce the problem of fitting of the structure to the electron density to simple fingerprint comparison. Using this approach, we are able to screen the entire Protein Data Bank and identify structures that fit two experimental electron densities determined by cryo-electron microscopy. PMID:21287628

Fingerprint-based structure retrieval using electron density.

PubMed

Yin, Shuangye; Dokholyan, Nikolay V

2011-03-01

We present a computational approach that can quickly search a large protein structural database to identify structures that fit a given electron density, such as determined by cryo-electron microscopy. We use geometric invariants (fingerprints) constructed using 3D Zernike moments to describe the electron density, and reduce the problem of fitting of the structure to the electron density to simple fingerprint comparison. Using this approach, we are able to screen the entire Protein Data Bank and identify structures that fit two experimental electron densities determined by cryo-electron microscopy. Copyright © 2010 Wiley-Liss, Inc.

Ultra-thin resin embedding method for scanning electron microscopy of individual cells on high and low aspect ratio 3D nanostructures.

PubMed

Belu, A; Schnitker, J; Bertazzo, S; Neumann, E; Mayer, D; Offenhäusser, A; Santoro, F

2016-07-01

The preparation of biological cells for either scanning or transmission electron microscopy requires a complex process of fixation, dehydration and drying. Critical point drying is commonly used for samples investigated with a scanning electron beam, whereas resin-infiltration is typically used for transmission electron microscopy. Critical point drying may cause cracks at the cellular surface and a sponge-like morphology of nondistinguishable intracellular compartments. Resin-infiltrated biological samples result in a solid block of resin, which can be further processed by mechanical sectioning, however that does not allow a top view examination of small cell-cell and cell-surface contacts. Here, we propose a method for removing resin excess on biological samples before effective polymerization. In this way the cells result to be embedded in an ultra-thin layer of epoxy resin. This novel method highlights in contrast to standard methods the imaging of individual cells not only on nanostructured planar surfaces but also on topologically challenging substrates with high aspect ratio three-dimensional features by scanning electron microscopy. © 2016 The Authors Journal of Microscopy © 2016 Royal Microscopical Society.

A national facility for biological cryo-electron microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Saibil, Helen R., E-mail: h.saibil@mail.cryst.bbk.ac.uk; Grünewald, Kay; Stuart, David I.

2015-01-01

This review provides a brief update on the use of cryo-electron microscopy for integrated structural biology, along with an overview of the plans for the UK national facility for electron microscopy being built at the Diamond synchrotron. Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an understanding of the molecular machinery of cells, disease processes and the actions of pathogenic organisms from atomic detail through to the cellular context. However, cutting-edge research in this field requires very substantial resources for equipment, infrastructure and expertise. Here, a brief overview is provided ofmore » the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. State-of-the-art equipment operated with expert support will be provided, optimized for both atomic-level single-particle analysis of purified macromolecules and complexes and for tomography of cell sections. The access to and organization of the facility will be modelled on the highly successful macromolecular crystallography (MX) synchrotron beamlines, and will be embedded at the Diamond Light Source, facilitating the development of user-friendly workflows providing near-real-time experimental feedback.« less

Relation Between Type II Bursts and CMEs Inferred from STEREO Observations

NASA Technical Reports Server (NTRS)

Gopalswamy, N.; Thompson, W.; Davila, J.; Kaiser, M. L.; Yashiro, S.; Maekelae, P.; Michalek, G.; Bougeret, J.-L.; Hoawrd, R. A.

2010-01-01

The inner coronagraph (COR1) of the Solar Terrestrial Relations Observatory (STEREO) mission has made it possible to observe coronal mass ejections (CMEs) a in the spatial domain overlapping with that of the metric type II radio bursts. The type II bursts were associated with generally weak flares (mostly B and C class soft X-ray flares), but the CMEs were quite energetic. Using CME data for a set of type II bursts during the declining phase of solar cycle 23, we determine the CME height when the type II bursts start, thus giving an estimate of the heliocentric distance at which CME-driven shocks form. This distance has been determined to be approximately 1.5Rs (solar radii), which coincides with the distance at which the Alfv?n speed profile has a minimum value. We also use type II radio observations from STEREO/WAVES and Wind/WAVES observations to show that CMEs with moderate speed drive either weak shocks or no shock at all when they attain a height where the Alfv?n speed peaks (?3Rs ? 4Rs). Thus the shocks seem to be most efficient in accelerating electrons in the heliocentric distance range of 1.5Rs to 4Rs. By combining the radial variation of the CME speed in the inner corona (CME speed increase) and interplanetary medium (speed decrease) we were able to correctly account for the deviations from the universal drift-rate spectrum of type II bursts, thus confirming the close physical connection between type II bursts and CMEs. The average height (approximately 1.5 Rs) of STEREO CMEs at the time of type II bursts is smaller than that (2.2 Rs) obtained for SOHO (Solar and Heliospheric Observatory) CMEs. We suggest that this may indicate, at least partly, the density reduction in the corona between the maximum and declining phases, so a given plasma level occurs closer to the Sun in the latter phase. In two cases, there was a diffuse shock-like feature ahead of the main body of the CME, indicating a standoff distance of 1Rs - 2Rs by the time the CME left the LASCO field of view.

Relation Between Type II Bursts and CMEs Inferred from STEREO Observations

NASA Technical Reports Server (NTRS)

Gopalswamy, N.; Thompson, W.; Davila, J.; Kaiser, M.; Yashiro, S.; Maelekae, P.; Michalek, G.; Bougret, J.-L.; Howard, R. A.

2009-01-01

The inner coronagraph (COR1) of the Solar Terrestrial Relations Observatory (STEREO) mission has made it possible to observe CMEs in the spatial domain overlapping with that of the metric type II radio bursts. The type II bursts were associated with generally weak flares (mostly B and C class soft X-ray flares), but the CMEs were quite energetic. Using CME data for a set of type II bursts during the declining phase of solar cycle 23, we determine the CME height when the type II bursts start, thus giving an estimate of the heliocentric distance at which CME-driven shocks form. This distance has been determined to be approx. 1.5Rs (solar radii), which coincides with the distance at which the Alfven speed profile has a minimum value.We also use type II radio observations from STEREO/WAVES and Wind/WAVES observations to show that CMEs with moderate speed drive either weak shocks or no shock at all when they attain a height where the Alfven speed peaks (approx. 3Rs - 4Rs). Thus the shocks seem to be most efficient in accelerating electrons in the heliocentric distance range of 1.5Rs to 4Rs. By combining the radial variation of the CME speed in the inner corona (CME speed increase) and interplanetary medium (speed decrease) we were able to correctly account for the deviations from the universal drift-rate spectrum of type II bursts, thus confirming the close physical connection between type II bursts and CMEs. The average height (approx 1.5Rs) of STEREO CMEs at the time of type II bursts is smaller than that (2.2Rs) obtained for SOHO (Solar and Heliospheric Observatory) CMEs. We suggest that this may indicate, at least partly, the density reduction in the corona between the maximum and declining phases, so a given plasma level occurs closer to the Sun in the latter phase. In two cases, there was a diffuse shock-like feature ahead of the main body of the CME, indicating a standoff distance of 1Rs - 2Rs by the time the CME left the LASCO field of view.

Transient flows of the solar wind associated with small-scale solar activity in solar minimum

NASA Astrophysics Data System (ADS)

Slemzin, Vladimir; Veselovsky, Igor; Kuzin, Sergey; Gburek, Szymon; Ulyanov, Artyom; Kirichenko, Alexey; Shugay, Yulia; Goryaev, Farid

The data obtained by the modern high sensitive EUV-XUV telescopes and photometers such as CORONAS-Photon/TESIS and SPHINX, STEREO/EUVI, PROBA2/SWAP, SDO/AIA provide good possibilities for studying small-scale solar activity (SSA), which is supposed to play an important role in heating of the corona and producing transient flows of the solar wind. During the recent unusually weak solar minimum, a large number of SSA events, such as week solar flares, small CMEs and CME-like flows were observed and recorded in the databases of flares (STEREO, SWAP, SPHINX) and CMEs (LASCO, CACTUS). On the other hand, the solar wind data obtained in this period by ACE, Wind, STEREO contain signatures of transient ICME-like structures which have shorter duration (<10h), weaker magnetic field strength (<10 nT) and lower proton temperature than usual ICMEs. To verify the assumption that ICME-like transients may be associated with the SSA events we investigated the number of weak flares of C-class and lower detected by SPHINX in 2009 and STEREO/EUVI in 2010. The flares were classified on temperature and emission measure using the diagnostic means of SPHINX and Hinode/EIS and were confronted with the parameters of the solar wind (velocity, density, ion composition and temperature, magnetic field, pitch angle distribution of the suprathermal electrons). The outflows of plasma associated with the flares were identified by their coronal signatures - CMEs (only in few cases) and dimmings. It was found that the mean parameters of the solar wind projected to the source surface for the times of the studied flares were typical for the ICME-like transients. The results support the suggestion that weak flares can be indicators of sources of transient plasma flows contributing to the slow solar wind at solar minimum, although these flows may be too weak to be considered as separate CMEs and ICMEs. The research leading to these results has received funding from the European Union’s Seventh Programme for Research, Technological Development and Demonstration under Grant Agreement “eHeroes” (project n° 284461, www.eheroes.eu).

Interplanetary shocks, Plasma waves and turbulence, Kinetic waves and instabilities, STEREO spacecraft

NASA Astrophysics Data System (ADS)

Cohen, Z.; Breneman, A. W.; Cattell, C. A.; Davis, L.; Grul, P.; Kersten, K.; Wilson, L. B., III

2017-12-01

Determining the role of plasma waves in providing energy dissipation at shock waves is of long-standing interest. Interplanetary (IP) shocks serve as a large database of low Mach number shocks. We examine electric field waveforms captured by the Time Domain Sampler (TDS) on the STEREO spacecraft during the ramps of IP shocks, with emphasis on captures lasting 2.1 seconds. Previous work has used captures of shorter duration (66 and 131 ms on STEREO, and 17 ms on WIND), which allowed for observation of waves with maximum (minimum) frequencies of 125 kHz (15 Hz), 62.5 kHz (8 Hz), and 60 kHz (59 Hz), respectively. The maximum frequencies are comparable to 2-8 times the plasma frequency in the solar wind, enabling observation of Langmuir waves, ion acoustic, and some whistler-mode waves. The 2 second captures resolve lower frequencies ( few Hz), which allows us to analyze packet structure of the whistler-mode waves and some ion acoustic waves. The longer capture time also improves the resolvability of simultaneous wave modes and of waves with frequencies on the order of 10s of Hz. Langmuir waves, however, cannot be identified at this sampling rate, since the plasma frequency is usually higher than 3.9 kHz. IP shocks are identified from multiple databases (Helsinki heliospheric shock database at http://ipshocks.fi, and the STEREO level 3 shock database at ftp://stereoftp.nascom.nasa.gov/pub/ins_data/impact/level3/). Our analysis focuses on TDS captures in shock ramp regions, with ramp durations determined from magnetic field data taken at 8 Hz. Software is used to identify multiple wave modes in any given capture and classify waves as Langmuir, ion acoustic, whistler, lower hybrid, electron cyclotron drift instability, or electrostatic solitary waves. Relevant frequencies are determined from density and magnetic field data collected in situ. Preliminary results suggest that large amplitude (∼ 5 mV/m) ion acoustic waves are most prevalent in the ramp, in agreement with Wilson, et al. Other modes are also observed. Statistical results will be presented and compared with previous studies and theoretical predictions.

STEREO Observations of Waves in the Ramp Regions of Interplanetary Shocks

NASA Astrophysics Data System (ADS)

Cohen, Z.; Breneman, A. W.; Cattell, C. A.; Davis, L.; Grul, P.; Kersten, K.; Wilson, L. B., III

2017-12-01

Determining the role of plasma waves in providing energy dissipation at shock waves is of long-standing interest. Interplanetary (IP) shocks serve as a large database of low Mach number shocks. We examine electric field waveforms captured by the Time Domain Sampler (TDS) on the STEREO spacecraft during the ramps of IP shocks, with emphasis on captures lasting 2.1 seconds. Previous work has used captures of shorter duration (66 and 131 ms on STEREO, and 17 ms on WIND), which allowed for observation of waves with maximum (minimum) frequencies of 125 kHz (15 Hz), 62.5 kHz (8 Hz), and 60 kHz (59 Hz), respectively. The maximum frequencies are comparable to 2-8 times the plasma frequency in the solar wind, enabling observation of Langmuir waves, ion acoustic, and some whistler-mode waves. The 2 second captures resolve lower frequencies ( few Hz), which allows us to analyze packet structure of the whistler-mode waves and some ion acoustic waves. The longer capture time also improves the resolvability of simultaneous wave modes and of waves with frequencies on the order of 10s of Hz. Langmuir waves, however, cannot be identified at this sampling rate, since the plasma frequency is usually higher than 3.9 kHz. IP shocks are identified from multiple databases (Helsinki heliospheric shock database at http://ipshocks.fi, and the STEREO level 3 shock database at ftp://stereoftp.nascom.nasa.gov/pub/ins_data/impact/level3/). Our analysis focuses on TDS captures in shock ramp regions, with ramp durations determined from magnetic field data taken at 8 Hz. Software is used to identify multiple wave modes in any given capture and classify waves as Langmuir, ion acoustic, whistler, lower hybrid, electron cyclotron drift instability, or electrostatic solitary waves. Relevant frequencies are determined from density and magnetic field data collected in situ. Preliminary results suggest that large amplitude (≥ 5 mV/m) ion acoustic waves are most prevalent in the ramp, in agreement with Wilson, et al. Other modes are also observed. Statistical results will be presented and compared with previous studies and theoretical predictions.

Electron Energy Loss Spectral Imaging of TiC Formed by Supernovae: A Scanning Transmission Electron Microscopy Study of Grain Formation and Alteration Mechanisms

NASA Astrophysics Data System (ADS)

Daulton, T. L.; Bernatowicz, T. J.; Croat, T. K.

2012-03-01

Micrometer-sized spherules of graphite formed by supernovae contain numerous TiC and Fe-Ni subgrains. These subgrains often have disordered surface rims. The mechanism(s) of rim formation on these subgrains is studied by transmission electron microscopy.

Characterization of Discontinuous Coarsening Reaction Products in INCONEL® Alloy 740H® Fusion Welds

NASA Astrophysics Data System (ADS)

Bechetti, Daniel H.; Dupont, John N.; Watanabe, Masashi; de Barbadillo, John J.

2017-04-01

Characterization of γ' coarsened zones (CZs) in alloy 740H fusion welds via a variety of electron microscopy techniques was conducted. The effects of solute partitioning during nonequilibrium solidification on the amount of strengthening precipitates along the grain boundaries were evaluated via electron-probe microanalysis and scanning electron microscopy. Electron backscatter diffraction was used to present evidence for the preferential growth of CZs toward regions of lower γ' content, even if growth in that direction increases grain boundary area. Scanning electron microscopy and image analysis were used to quantify the propensity for CZs to develop along certain segments of the grain boundaries, as governed by the local variations in γ' content. Scanning transmission electron microscopy with X-ray energy-dispersive spectrometry (XEDS) was used to assess the compositions of the matrix and precipitate phases within the CZs and to quantify the segregation of alloying components to the reaction front. Thermodynamic and kinetic modeling were used to compare calculated and experimental compositions. The work presented here provides new insight into the progression of the discontinuous coarsening (DC) reaction in a complex engineering alloy.

Characterization of Sulfur and Nanostructured Sulfur Battery Cathodes in Electron Microscopy Without Sublimation Artifacts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Levin, Barnaby D. A.; Zachman, Michael J.; Werner, Jörg G.

Abstract Lithium sulfur (Li–S) batteries have the potential to provide higher energy storage density at lower cost than conventional lithium ion batteries. A key challenge for Li–S batteries is the loss of sulfur to the electrolyte during cycling. This loss can be mitigated by sequestering the sulfur in nanostructured carbon–sulfur composites. The nanoscale characterization of the sulfur distribution within these complex nanostructured electrodes is normally performed by electron microscopy, but sulfur sublimates and redistributes in the high-vacuum conditions of conventional electron microscopes. The resulting sublimation artifacts render characterization of sulfur in conventional electron microscopes problematic and unreliable. Here, we demonstratemore » two techniques, cryogenic transmission electron microscopy (cryo-TEM) and scanning electron microscopy in air (airSEM), that enable the reliable characterization of sulfur across multiple length scales by suppressing sulfur sublimation. We use cryo-TEM and airSEM to examine carbon–sulfur composites synthesized for use as Li–S battery cathodes, noting several cases where the commonly employed sulfur melt infusion method is highly inefficient at infiltrating sulfur into porous carbon hosts.« less

Insights into radiation damage from atomic resolution scanning transmission electron microscopy imaging of mono-layer CuPcCl16 films on graphene.

PubMed

Mittelberger, Andreas; Kramberger, Christian; Meyer, Jannik C

2018-03-19

Atomically resolved images of monolayer organic crystals have only been obtained with scanning probe methods so far. On the one hand, they are usually prepared on surfaces of bulk materials, which are not accessible by (scanning) transmission electron microscopy. On the other hand, the critical electron dose of a monolayer organic crystal is orders of magnitudes lower than the one for bulk crystals, making (scanning) transmission electron microscopy characterization very challenging. In this work we present an atomically resolved study on the dynamics of a monolayer CuPcCl 16 crystal under the electron beam as well as an image of the undamaged molecules obtained by low-dose electron microscopy. The results show the dynamics and the radiation damage mechanisms in the 2D layer of this material, complementing what has been found for bulk crystals in earlier studies. Furthermore, being able to image the undamaged molecular crystal allows the characterization of new composites consisting of 2D materials and organic molecules.

Environmental scanning electron microscopy in cell biology.

PubMed

McGregor, J E; Staniewicz, L T L; Guthrie Neé Kirk, S E; Donald, A M

2013-01-01

Environmental scanning electron microscopy (ESEM) (1) is an imaging technique which allows hydrated, insulating samples to be imaged under an electron beam. The resolution afforded by this technique is higher than conventional optical microscopy but lower than conventional scanning electron microscopy (CSEM). The major advantage of the technique is the minimal sample preparation needed, making ESEM quick to use and the images less susceptible to the artifacts that the extensive sample preparation usually required for CSEM may introduce. Careful manipulation of both the humidity in the microscope chamber and the beam energy are nevertheless essential to prevent dehydration and beam damage artifacts. In some circumstances it is possible to image live cells in the ESEM (2).In the following sections we introduce the fundamental principles of ESEM imaging before presenting imaging protocols for plant epidermis, mammalian cells, and bacteria. In the first two cases samples are imaged using the secondary electron (topographic) signal, whereas a transmission technique is employed to image bacteria.

Software electron counting for low-dose scanning transmission electron microscopy.

PubMed

Mittelberger, Andreas; Kramberger, Christian; Meyer, Jannik C

2018-05-01

The performance of the detector is of key importance for low-dose imaging in transmission electron microscopy, and counting every single electron can be considered as the ultimate goal. In scanning transmission electron microscopy, low-dose imaging can be realized by very fast scanning, however, this also introduces artifacts and a loss of resolution in the scan direction. We have developed a software approach to correct for artifacts introduced by fast scans, making use of a scintillator and photomultiplier response that extends over several pixels. The parameters for this correction can be directly extracted from the raw image. Finally, the images can be converted into electron counts. This approach enables low-dose imaging in the scanning transmission electron microscope via high scan speeds while retaining the image quality of artifact-free slower scans. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Unstructured Facility Navigation by Applying the NIST 4D/RCS Architecture

DTIC Science & Technology

2006-07-01

control, and the planner); wire- less data and emergency stop radios; GPS receiver; inertial navigation unit; dual stereo cameras; infrared sensors...current Actuators Wheel motors, camera controls Scale & filter signals status commands commands commands GPS Antenna Dual stereo cameras...used in the sensory processing module include the two pairs of stereo color cameras, the physical bumper and infrared bumper sensors, the motor

Topography from shading and stereo

NASA Technical Reports Server (NTRS)

Horn, Berthold K. P.

1994-01-01

Methods exploiting photometric information in images that have been developed in machine vision can be applied to planetary imagery. Integrating shape from shading, binocular stereo, and photometric stereo yields a robust system for recovering detailed surface shape and surface reflectance information. Such a system is useful in producing quantitative information from the vast volume of imagery being received, as well as in helping visualize the underlying surface.

Self calibration of the stereo vision system of the Chang'e-3 lunar rover based on the bundle block adjustment

NASA Astrophysics Data System (ADS)

Zhang, Shuo; Liu, Shaochuang; Ma, Youqing; Qi, Chen; Ma, Hao; Yang, Huan

2017-06-01

The Chang'e-3 was the first lunar soft landing probe of China. It was composed of the lander and the lunar rover. The Chang'e-3 successful landed in the northwest of the Mare Imbrium in December 14, 2013. The lunar rover completed the movement, imaging and geological survey after landing. The lunar rover equipped with a stereo vision system which was made up of the Navcam system, the mast mechanism and the inertial measurement unit (IMU). The Navcam system composed of two cameras with the fixed focal length. The mast mechanism was a robot with three revolute joints. The stereo vision system was used to determine the position of the lunar rover, generate the digital elevation models (DEM) of the surrounding region and plan the moving paths of the lunar rover. The stereo vision system must be calibrated before use. The control field could be built to calibrate the stereo vision system in the laboratory on the earth. However, the parameters of the stereo vision system would change after the launch, the orbital changes, the braking and the landing. Therefore, the stereo vision system should be self calibrated on the moon. An integrated self calibration method based on the bundle block adjustment is proposed in this paper. The bundle block adjustment uses each bundle of ray as the basic adjustment unit and the adjustment is implemented in the whole photogrammetric region. The stereo vision system can be self calibrated with the proposed method under the unknown lunar environment and all parameters can be estimated simultaneously. The experiment was conducted in the ground lunar simulation field. The proposed method was compared with other methods such as the CAHVOR method, the vanishing point method, the Denavit-Hartenberg method, the factorization method and the weighted least-squares method. The analyzed result proved that the accuracy of the proposed method was superior to those of other methods. Finally, the proposed method was practical used to self calibrate the stereo vision system of the Chang'e-3 lunar rover on the moon.

Correlative light-electron fractography for fatigue striations characterization in metallic alloys.

PubMed

Hein, Luis Rogerio de Oliveira; de Oliveira, José Alberto; de Campos, Kamila Amato

2013-09-01

The correlative light-electron fractography technique combines correlative microscopy concepts to the extended depth-from-focus reconstruction method, associating the reliable topographic information of 3-D maps from light microscopy ordered Z-stacks to the finest lateral resolution and large focus depth from scanning electron microscopy. Fatigue striations spacing analysis can be precisely measured, by correcting the mean surface tilting with the knowledge of local elevation data from elevation maps. This new technique aims to improve the accuracy of quantitative fractography in fatigue fracture investigations. Copyright © 2013 Wiley Periodicals, Inc.

Transmission Electron Microscopy Analysis of Skin Lesions from Sporotrichosis Epidemic in Rio de Janeiro, Brazil

PubMed Central

Porto Ferreira, Cassio; Oliveira de Almeida, Ana Cristina; Corte-Real, Suzana

2015-01-01

Transmission electron microscopy can yield useful information in a range of scientific fields; it is capable of imaging at a significantly higher resolution than light microscopes and has been a very useful tool in the identification of morphological changes of the dermis as well as assessment of changes in the extracellular matrix. Our aim is to characterize by electron microscopy the cellular profile of lesions caused by Sporothrix schenckii from the sporotrichosis epidemic in its zoonotic form that occurs in Rio de Janeiro, Brazil. PMID:25653392

Surface ultrastructural (SEM) characteristics of oropharyngeal cavity of house sparrow (Passer domesticus).

PubMed

Abumandour, Mohamed M A

2018-06-01

The focus of the present study is to provide a full morphological description of the oropharyngeal cavity of the house sparrow. The head of six birds was prepared for gross examination and by stereo and electron microscopy. The bifid lingual apex has multiple long, rostrally directed needle-like processes. The lateral border of the apex carries rostromedially directed needle-like processes. The dorsal lingual surface of the apex and body carries numerous caudomedially directed filiform papillae and many orifices of lingual salivary glands. The lingual body is divided into two parts: rostral and caudal. The caudal part is divided into two laterally elevated regions by a median groove, while the rostral part is bounded laterally by a rostrodorsally directed papillary row, which on SEM is formed from two rows. On SEM, the lingual root has many orifices of posterior salivary glands. The pharyngeal papillary row is located at the caudal border of the laryngeal mound, but this single papillary row is formed from two rows at SEM magnification. The laryngeal cleft continues caudally as a laryngeal fissure bounded by two longitudinal rows of caudally directed papillae; at high SEM magnification, this fissure is divided into two halves by a median ridge which carries caudally directed papillae on its posterior part. The choanal cleft proceeds rostrally by the median tubercle. There are a small number of orifices of palatine salivary glands. The morphological characters of the oropharyngeal cavity of the sparrow confirm its adaptation to surrounding environmental conditions and available food particles.

Effect of negative bias on TiAlSiN coating deposited on nitrided Zircaloy-4

NASA Astrophysics Data System (ADS)

Jun, Zhou; Zhendong, Feng; Xiangfang, Fan; Yanhong, Liu; Huanlin, Li

2018-01-01

TiAlSiN coatings were deposited on the nitrided Zircaloy-4 by multi-arc ion plating at -100 V, -200 V and -300 V. In this study, the high temperature oxidation behavior of coatings was tested by a box-type resistance furnace in air for 3 h at 800 °C; the macro-morphology of coatings was observed and analyzed by a zoom-stereo microscope; the micro-morphology of coatings was analyzed by a scanning electron microscopy (SEM), and the chemical elements of samples were analyzed by an energy dispersive spectroscopy(EDS); the adhesion strength of the coating to the substrate was measured by an automatic scratch tester; and the phases of coatings were analyzed by an X-ray diffractometer(XRD). Results show that the coating deposited at -100 V shows better high temperature oxidation resistance behavior, at the same time, Al elements contained in the coating is of the highest amount, meanwhile, the adhesion strength of the coating to the substrate is the highest, which is 33N. As the bias increases, high temperature oxidation resistance behavior of the coating weakens first and then increases, the amount of large particles on the surface of the coating increases first and then decreases whereas the density of the coating decreases first and then increases, and adhesion strength of the coating to the substrate increases first and then weakens. The coating's quality is relatively poor when the bias is -200 V.

3D reconstruction of SEM images by use of optical photogrammetry software.

PubMed

Eulitz, Mona; Reiss, Gebhard

2015-08-01

Reconstruction of the three-dimensional (3D) surface of an object to be examined is widely used for structure analysis in science and many biological questions require information about their true 3D structure. For Scanning Electron Microscopy (SEM) there has been no efficient non-destructive solution for reconstruction of the surface morphology to date. The well-known method of recording stereo pair images generates a 3D stereoscope reconstruction of a section, but not of the complete sample surface. We present a simple and non-destructive method of 3D surface reconstruction from SEM samples based on the principles of optical close range photogrammetry. In optical close range photogrammetry a series of overlapping photos is used to generate a 3D model of the surface of an object. We adapted this method to the special SEM requirements. Instead of moving a detector around the object, the object itself was rotated. A series of overlapping photos was stitched and converted into a 3D model using the software commonly used for optical photogrammetry. A rabbit kidney glomerulus was used to demonstrate the workflow of this adaption. The reconstruction produced a realistic and high-resolution 3D mesh model of the glomerular surface. The study showed that SEM micrographs are suitable for 3D reconstruction by optical photogrammetry. This new approach is a simple and useful method of 3D surface reconstruction and suitable for various applications in research and teaching. Copyright © 2015 Elsevier Inc. All rights reserved.

Relative merits and limiting factors for x-ray and electron microscopy of thick, hydrated organic materials

DOE PAGES

Du, Ming; Jacobsen, Chris

2017-10-07

Electron and x-ray microscopes allow one to image the entire, unlabeled structure of hydrated materials at a resolution well beyond what visible light microscopes can achieve. However, both approaches involve ionizing radiation, so that radiation damage must be considered as one of the limits to imaging. Drawing upon earlier work, we describe here a unified approach to estimating the image contrast (and thus the required exposure and corresponding radiation dose) in both x-ray and electron microscopy. This approach accounts for factors such as plural and inelastic scattering, and (in electron microscopy) the use of energy filters to obtain so-called "zeromore » loss" images. As expected, it shows that electron microscopy offers lower dose for specimens thinner than about 1 mu m (such as for studies of macromolecules, viruses, bacteria and archaebacteria, and thin sectioned material), while x-ray microscopy offers superior characteristics for imaging thicker specimen such as whole eukaryotic cells, thick-sectioned tissues, and organs. The required radiation dose scales strongly as a function of the desired spatial resolution, allowing one to understand the limits of live and frozen hydrated specimen imaging. Lastly, we consider the factors limiting x-ray microscopy of thicker materials, suggesting that specimens as thick as a whole mouse brain can be imaged with x-ray microscopes without significant image degradation should appropriate image reconstruction methods be identified.« less

Relative merits and limiting factors for x-ray and electron microscopy of thick, hydrated organic materials

DOE Office of Scientific and Technical Information (OSTI.GOV)

Du, Ming; Jacobsen, Chris

Electron and x-ray microscopes allow one to image the entire, unlabeled structure of hydrated materials at a resolution well beyond what visible light microscopes can achieve. However, both approaches involve ionizing radiation, so that radiation damage must be considered as one of the limits to imaging. Drawing upon earlier work, we describe here a unified approach to estimating the image contrast (and thus the required exposure and corresponding radiation dose) in both x-ray and electron microscopy. This approach accounts for factors such as plural and inelastic scattering, and (in electron microscopy) the use of energy filters to obtain so-called "zeromore » loss" images. As expected, it shows that electron microscopy offers lower dose for specimens thinner than about 1 mu m (such as for studies of macromolecules, viruses, bacteria and archaebacteria, and thin sectioned material), while x-ray microscopy offers superior characteristics for imaging thicker specimen such as whole eukaryotic cells, thick-sectioned tissues, and organs. The required radiation dose scales strongly as a function of the desired spatial resolution, allowing one to understand the limits of live and frozen hydrated specimen imaging. Lastly, we consider the factors limiting x-ray microscopy of thicker materials, suggesting that specimens as thick as a whole mouse brain can be imaged with x-ray microscopes without significant image degradation should appropriate image reconstruction methods be identified.« less

Microscopy with slow electrons: from LEEM to XPEEM

ScienceCinema

Bauer, Ernst [Arizona State University, Phoenix, Arizona, United States

2017-12-09

The short penetration and escape depth of electrons with energies below 1 keV make them ideally suited for the study of surfaces and ultrathin films. The combination of the low energy electrons and the high lateral resolution of a microscope produces a powerful method for the characterization of nanostructures on bulk samples, in particular if the microscope is equipped with an imaging energy filter and connected to a synchrotron radiation source. Comprehensive characterization by imaging, diffraction, and spectroscope of the structural, chemical, and magnetic properties is then possible. The Talk will describe the various imaging techniques in using reflected and emitted electrons in low-energy electron microscopy (LEEM) and x-ray photoemission electron microscopy (XPEEM), with an emphasis on magnetic materials with spin-polarized LEEM and x-ray magnetic circular dichroism PEEM. The talk with end with an outlook on future possibilities.

Stereo images from space

NASA Astrophysics Data System (ADS)

Sabbatini, Massimo; Collon, Maximilien J.; Visentin, Gianfranco

2008-02-01

The Erasmus Recording Binocular (ERB1) was the first fully digital stereo camera used on the International Space Station. One year after its first utilisation, the results and feedback collected with various audiences have convinced us to continue exploiting the outreach potential of such media, with its unique capability to bring space down to earth, to share the feeling of weightlessness and confinement with the viewers on earth. The production of stereo is progressing quickly but it still poses problems for the distribution of the media. The Erasmus Centre of the European Space Agency has experienced how difficult it is to master the full production and distribution chain of a stereo system. Efforts are also on the way to standardize the satellite broadcasting part of the distribution. A new stereo camera is being built, ERB2, to be launched to the International Space Station (ISS) in September 2008: it shall have 720p resolution, it shall be able to transmit its images to the ground in real-time allowing the production of live programs and it could possibly be used also outside the ISS, in support of Extra Vehicular Activities of the astronauts. These new features are quite challenging to achieve in the reduced power and mass budget available to space projects and we hope to inspire more designers to come up with ingenious ideas to built cameras capable to operate in the hash Low Earth Orbit environment: radiations, temperature, power consumption and thermal design are the challenges to be met. The intent of this paper is to share with the readers the experience collected so far in all aspects of the 3D video production chain and to increase awareness on the unique content that we are collecting: nice stereo images from space can be used by all actors in the stereo arena to gain consensus on this powerful media. With respect to last year we shall present the progress made in the following areas: a) the satellite broadcasting live of stereo content to D-Cinema's in Europe; b) the design challenges to fly the camera outside the ISS as opposed to ERB1 that was only meant to be used in the pressurized environment of the ISS; c) on-board stereo viewing on a stereo camera has been tackled in ERB1: trade offs between OLED and LCOS display technologies shall be presented; d) HD_SDI cameras versus USB2 or Firewire; e) the hardware compression ASIC solutions used to tackle the high data rate on-board; f) 3D geometry reconstruction: first attempts in reconstructing a computer model of the interior of the ISS starting form the stereo video available.

Microscopy image segmentation tool: Robust image data analysis

NASA Astrophysics Data System (ADS)

Valmianski, Ilya; Monton, Carlos; Schuller, Ivan K.

2014-03-01

We present a software package called Microscopy Image Segmentation Tool (MIST). MIST is designed for analysis of microscopy images which contain large collections of small regions of interest (ROIs). Originally developed for analysis of porous anodic alumina scanning electron images, MIST capabilities have been expanded to allow use in a large variety of problems including analysis of biological tissue, inorganic and organic film grain structure, as well as nano- and meso-scopic structures. MIST provides a robust segmentation algorithm for the ROIs, includes many useful analysis capabilities, and is highly flexible allowing incorporation of specialized user developed analysis. We describe the unique advantages MIST has over existing analysis software. In addition, we present a number of diverse applications to scanning electron microscopy, atomic force microscopy, magnetic force microscopy, scanning tunneling microscopy, and fluorescent confocal laser scanning microscopy.

The foliar trichomes of Hypoestes aristata (Vahl) Sol. ex Roem. & Schult var aristata (Acanthaceae) a widespread medicinal plant species in tropical sub-Saharan Africa: with comments on its possible phylogenetic significance.

PubMed

Bhatt, A; Naidoo, Y; Nicholas, A

2010-01-01

The micromorphology of foliar trichomes of Hypoestes aristata var. aristata was studied using stereo, light and scanning microscopy (SEM). This genus belongs to the advanced angiosperm family Acanthaceae, for which few micromorphological leaf studies exist. Results revealed both glandular and non-glandular trichomes, the latter being more abundant on leaf veins, particularly on the abaxial surface of very young leaves. With leaf maturity, the density of non-glandular trichomes decreased. Glandular trichomes were rare and of two types: long-stalked capitate and globose-like peltate trichomes. Capitate trichomes were observed only on the abaxial leaf surface, while peltate trichomes were distributed on both adaxial and abaxial leaf surfaces.

Snow White Trench Prepared for Sample Collection

NASA Technical Reports Server (NTRS)

2008-01-01

The informally named 'Snow White' trench is the source for the next sample to be acquired by NASA's Phoenix Mars Lander for analysis by the wet chemistry lab.

The Surface Stereo Imager on Phoenix took this shadow-enhanced image of the trench, on the eastern end of Phoenix's work area, on Sol 103, or the 103rd day of the mission, Sept. 8, 2008. The trench is about 23 centimeters (9 inches) wide.

The wet chemistry lab is part of Phoenix's Microscopy, Electrochemistry and Conductivity suite of instruments.

The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

The relationship of acquisition systems to automated stereo correlation.

USGS Publications Warehouse

Colvocoresses, A.P.

1983-01-01

Today a concerted effort is being made to expedite the mapping process through automated correlation of stereo data. Stereo correlation involves the comparison of radiance (brightness) signals or patterns recorded by sensors. Conventionally, two-dimensional area correlation is utilized but this is a rather slow and cumbersome procedure. Digital correlation can be performed in only one dimension where suitable signal patterns exist, and the one-dimensional mode is much faster. Electro-optical (EO) systems, suitable for space use, also have much greater flexibility than film systems. Thus, an EO space system can be designed which will optimize one-dimensional stereo correlation and lead toward the automation of topographic mapping.-from Author

Stereoscopic image production: live, CGI, and integration

NASA Astrophysics Data System (ADS)

Criado, Enrique

2006-02-01

This paper shortly describes part of the experience gathered in more than 10 years of stereoscopic movie production, some of the most common problems found and the solutions, with more or less fortune, we applied to solve those problems. Our work is mainly focused in the entertainment market, theme parks, museums, and other cultural related locations and events. In our movies, we have been forced to develop our own devices to permit correct stereo shooting (stereoscopic rigs) or stereo monitoring (real-time), and to solve problems found with conventional film editing, compositing and postproduction software. Here, we discuss stereo lighting, monitoring, special effects, image integration (using dummies and more), stereo-camera parameters, and other general 3-D movie production aspects.

Optics of the human cornea influence the accuracy of stereo eye-tracking methods: a simulation study.

PubMed

Barsingerhorn, A D; Boonstra, F N; Goossens, H H L M

2017-02-01

Current stereo eye-tracking methods model the cornea as a sphere with one refractive surface. However, the human cornea is slightly aspheric and has two refractive surfaces. Here we used ray-tracing and the Navarro eye-model to study how these optical properties affect the accuracy of different stereo eye-tracking methods. We found that pupil size, gaze direction and head position all influence the reconstruction of gaze. Resulting errors range between ± 1.0 degrees at best. This shows that stereo eye-tracking may be an option if reliable calibration is not possible, but the applied eye-model should account for the actual optics of the cornea.

KSC-06pd1869

NASA Image and Video Library

2006-08-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the STEREO payload is ready for mating to the upper stage booster. Here, a crane is being attached to the spacecraft to lift it. After the mating, all will be transported to Launch Pad 17-B at Cape Canaveral Air Force Station for integration with the Delta II already on the pad. STEREO stands for Solar Terrestrial Relations Observatory and comprises two spacecraft. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off Aug. 31. Photo credit: NASA/George Shelton

KSC-06pd1159

NASA Image and Video Library

2006-06-16

KENNEDY SPACE CENTER, FLA. - At Astrotech Space Operations in Titusville, Fla., the tilt table lowers the STEREO spacecraft "A." In this position, technicians can perform the final comprehensive performance test of the instruments, verifying the instrument is fully functional before flight. After a rotation, this configuration also allows deployment tests to be done on the solar arrays. STEREO stands for Solar Terrestrial Relations Observatory. The STEREO mission is the first to take measurements of the sun and solar wind in 3-dimension. This new view will improve our understanding of space weather and its impact on the Earth. STEREO is expected to lift off aboard a Boeing Delta II rocket on July 22. Photo credit: NASA/George Shelton

3D optic disc reconstruction via a global fundus stereo algorithm.

PubMed

Bansal, M; Sizintsev, M; Eledath, J; Sawhney, H; Pearson, D J; Stone, R A

2013-01-01

This paper presents a novel method to recover 3D structure of the optic disc in the retina from two uncalibrated fundus images. Retinal images are commonly uncalibrated when acquired clinically, creating rectification challenges as well as significant radiometric and blur differences within the stereo pair. By exploiting structural peculiarities of the retina, we modified the Graph Cuts computational stereo method (one of current state-of-the-art methods) to yield a high quality algorithm for fundus stereo reconstruction. Extensive qualitative and quantitative experimental evaluation (where OCT scans are used as 3D ground truth) on our and publicly available datasets shows the superiority of the proposed method in comparison to other alternatives.

Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points

PubMed Central

Karreman, Matthia A.; Mercier, Luc; Schieber, Nicole L.; Shibue, Tsukasa; Schwab, Yannick; Goetz, Jacky G.

2014-01-01

Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient events at high resolution. Performing correlative microscopy in complex and bulky samples such as an entire living organism is a time-consuming and error-prone task. Here, we investigate correlative methods that rely on the use of artificial and endogenous structural features of the sample as reference points for correlating intravital fluorescence microscopy and electron microscopy. To investigate tumor cell behavior in vivo with ultrastructural accuracy, a reliable approach is needed to retrieve single tumor cells imaged deep within the tissue. For this purpose, fluorescently labeled tumor cells were subcutaneously injected into a mouse ear and imaged using two-photon-excitation microscopy. Using near-infrared branding, the position of the imaged area within the sample was labeled at the skin level, allowing for its precise recollection. Following sample preparation for electron microscopy, concerted usage of the artificial branding and anatomical landmarks enables targeting and approaching the cells of interest while serial sectioning through the specimen. We describe here three procedures showing how three-dimensional (3D) mapping of structural features in the tissue can be exploited to accurately correlate between the two imaging modalities, without having to rely on the use of artificially introduced markers of the region of interest. The methods employed here facilitate the link between intravital and nanoscale imaging of invasive tumor cells, enabling correlating function to structure in the study of tumor invasion and metastasis. PMID:25479106

Publications - GMC 58 | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

DGGS GMC 58 Publication Details Title: X-ray diffraction and scanning electron microscopy mineral , Michael, and Core Laboratories, 1985, X-ray diffraction and scanning electron microscopy mineral analyses

Nanodiamond Landmarks for Subcellular Multimodal Optical and Electron Imaging

PubMed Central

Zurbuchen, Mark A.; Lake, Michael P.; Kohan, Sirus A.; Leung, Belinda; Bouchard, Louis-S.

2013-01-01

There is a growing need for biolabels that can be used in both optical and electron microscopies, are non-cytotoxic, and do not photobleach. Such biolabels could enable targeted nanoscale imaging of sub-cellular structures, and help to establish correlations between conjugation-delivered biomolecules and function. Here we demonstrate a sub-cellular multi-modal imaging methodology that enables localization of inert particulate probes, consisting of nanodiamonds having fluorescent nitrogen-vacancy centers. These are functionalized to target specific structures, and are observable by both optical and electron microscopies. Nanodiamonds targeted to the nuclear pore complex are rapidly localized in electron-microscopy diffraction mode to enable “zooming-in” to regions of interest for detailed structural investigations. Optical microscopies reveal nanodiamonds for in-vitro tracking or uptake-confirmation. The approach is general, works down to the single nanodiamond level, and can leverage the unique capabilities of nanodiamonds, such as biocompatibility, sensitive magnetometry, and gene and drug delivery. PMID:24036840

Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells

PubMed Central

Hampton, Cheri M; Strauss, Joshua D; Ke, Zunlong; Dillard, Rebecca S; Hammonds, Jason E; Alonas, Eric; Desai, Tanay M; Marin, Mariana; Storms, Rachel E; Leon, Fredrick; Melikyan, Gregory B; Santangelo, Philip J; Spearman, Paul W; Wright, Elizabeth R

2016-01-01

Correlative light and electron microscopy (CLEM) combines spatiotemporal information from fluorescence light microscopy (fLM) with high-resolution structural data from cryo-electron tomography (cryo-ET). These technologies provide opportunities to bridge knowledge gaps between cell and structural biology. Here we describe our protocol for correlated cryo-fLM, cryo-electron microscopy (cryo-EM), and cryo-ET (i.e., cryo-CLEM) of virus-infected or transfected mammalian cells. Mammalian-derived cells are cultured on EM substrates, using optimized conditions that ensure that the cells are spread thinly across the substrate and are not physically disrupted. The cells are then screened by fLM and vitrified before acquisition of cryo-fLM and cryo-ET images, which is followed by data processing. A complete session from grid preparation through data collection and processing takes 5–15 d for an individual experienced in cryo-EM. PMID:27977021

In situ transmission electron microscopy of transistor operation and failure.

PubMed

Wang, Baoming; Islam, Zahabul; Haque, Aman; Chabak, Kelson; Snure, Michael; Heller, Eric; Glavin, Nicholas

2018-08-03

Microscopy is typically used as a post-mortem analytical tool in performance and reliability studies on nanoscale materials and devices. In this study, we demonstrate real time microscopy of the operation and failure of AlGaN/GaN high electron mobility transistors inside the transmission electron microscope. Loading until failure was performed on the electron transparent transistors to visualize the failure mechanisms caused by self-heating. At lower drain voltages, thermo-mechanical stresses induce irreversible microstructural deformation, mostly along the AlGaN/GaN interface, to initiate the damage process. At higher biasing, the self-heating deteriorates the gate and catastrophic failure takes place through metal/semiconductor inter-diffusion and/or buffer layer breakdown. This study indicates that the current trend of recreating the events, from damage nucleation to catastrophic failure, can be replaced by in situ microscopy for a quick and accurate account of the failure mechanisms.

Low-voltage electron microscopy of polymer and organic molecular thin films.

PubMed

Drummy, Lawrence F; Yang, Junyan; Martin, David C

2004-06-01

We have demonstrated the capabilities of a novel low-voltage electron microscope (LVEM) for imaging polymer and organic molecular thin films. The LVEM can operate in transmission electron microscopy, scanning transmission electron microscopy, scanning electron microscopy, and electron diffraction modes. The microscope operates at a nominal accelerating voltage of 5 kV and fits on a tabletop. A detailed discussion of the electron-sample interaction processes is presented, and the mean free path for total electron scattering was calculated to be 15 nm for organic samples at 5 kV. The total end point dose for the destruction of crystallinity at 5 kV was estimated at 5 x 10(-4) and 3.5 x 10(-2) C/cm2 for polyethylene and pentacene, respectively. These values are significantly lower than those measured at voltages greater than 100 kV. A defocus series of colloidal gold particles allowed us to estimate the experimental contrast transfer function of the microscope. Images taken of several organic materials have shown high contrast for low atomic number elements and a resolution of 2.5 nm. The materials studied here include thin films of the organic semiconductor pentacene, triblock copolymer films, single-molecule dendrimers, electrospun polymer fibers and gold nanoparticles. Copyright 2004 Elsevier B.V.

[THE CHARACTERISTICS OF MORPHOLOGY OF BIOFILM OF PERIODONTIUM UNDER INFLAMMATORY DISEASES OF GUMS (CHRONIC CATARRHAL GINGIVITIS, CHRONIC PERIODONTITIS, CANDIDA-ASSOCIATED PERIODONTITIS) ACCORDING RESULTS OF ELECTRONIC MICROSCOPY].

PubMed

Ippolitov, E V; Didenko, L V; Tzarev, V N

2015-12-01

The study was carried out to analyze morphology of biofilm of periodontium and to develop electronic microscopic criteria of differentiated diagnostic of inflammatory diseases of gums. The scanning electronic microscopy was applied to analyze samples of bioflm of periodont from 70 patients. Including ten patients with every nosologic form of groups with chronic catarrhal periodontitis. of light, mean and severe degree, chronic catarrhal gingivitis, Candida-associated paroperiodontitis and 20 healthy persons with intact periodontium. The analysis was implemented using dual-beam scanning electronic microscope Quanta 200 3D (FEI company, USA) and walk-through electronic micJEM 100B (JEOL, Japan). To detect marker DNA of periodont pathogenic bacteria in analyzed samples the kit of reagentsfor polymerase chain reaction "MultiDent-5" ("GenLab", Russia). The scanning electronic microscopy in combination with transmission electronic microscopy and polymerase chain reaction permits analyzing structure, composition and degree of development of biofilm of periodontium and to apply differentiated diagnostic of different nosologic forms of inflammatory diseases of periodontium, including light form of chronic periodontitis and gingivitis. The electronic microscopical indications of diseases ofperiodontium of inflammatory character are established: catarrhal gingivitis, (coccal morphological alternate), chronic periodontitis (bacillary morphological alternate), Candida-associated periodontitis (Candida morphological alternate of biofilm ofperiodontium).

Depth-Based Selective Blurring in Stereo Images Using Accelerated Framework

NASA Astrophysics Data System (ADS)

Mukherjee, Subhayan; Guddeti, Ram Mohana Reddy

2014-09-01

We propose a hybrid method for stereo disparity estimation by combining block and region-based stereo matching approaches. It generates dense depth maps from disparity measurements of only 18 % image pixels (left or right). The methodology involves segmenting pixel lightness values using fast K-Means implementation, refining segment boundaries using morphological filtering and connected components analysis; then determining boundaries' disparities using sum of absolute differences (SAD) cost function. Complete disparity maps are reconstructed from boundaries' disparities. We consider an application of our method for depth-based selective blurring of non-interest regions of stereo images, using Gaussian blur to de-focus users' non-interest regions. Experiments on Middlebury dataset demonstrate that our method outperforms traditional disparity estimation approaches using SAD and normalized cross correlation by up to 33.6 % and some recent methods by up to 6.1 %. Further, our method is highly parallelizable using CPU-GPU framework based on Java Thread Pool and APARAPI with speed-up of 5.8 for 250 stereo video frames (4,096 × 2,304).

Clinical applications of modern imaging technology: stereo image formation and location of brain cancer

NASA Astrophysics Data System (ADS)

Wang, Dezong; Wang, Jinxiang

1994-05-01

It is very important to locate the tumor for a patient, who has cancer in his brain. If he only gets X-CT or MRI pictures, the doctor does not know the size, shape location of the tumor and the relation between the tumor and other organs. This paper presents the formation of stereo images of cancer. On the basis of color code and color 3D reconstruction. The stereo images of tumor, brain and encephalic truncus are formed. The stereo image of cancer can be round on X, Y, Z-coordinates to show the shape from different directions. In order to show the location of tumor, stereo image of tumor and encephalic truncus are provided on different angles. The cross section pictures are also offered to indicate the relation of brain, tumor and encephalic truncus on cross sections. In this paper the calculating of areas, volume and the space between cancer and the side of the brain are also described.

Novel Descattering Approach for Stereo Vision in Dense Suspended Scatterer Environments

PubMed Central

Nguyen, Chanh D. Tr.; Park, Jihyuk; Cho, Kyeong-Yong; Kim, Kyung-Soo; Kim, Soohyun

2017-01-01

In this paper, we propose a model-based scattering removal method for stereo vision for robot manipulation in indoor scattering media where the commonly used ranging sensors are unable to work. Stereo vision is an inherently ill-posed and challenging problem. It is even more difficult in the case of images of dense fog or dense steam scenes illuminated by active light sources. Images taken in such environments suffer attenuation of object radiance and scattering of the active light sources. To solve this problem, we first derive the imaging model for images taken in a dense scattering medium with a single active illumination close to the cameras. Based on this physical model, the non-uniform backscattering signal is efficiently removed. The descattered images are then utilized as the input images of stereo vision. The performance of the method is evaluated based on the quality of the depth map from stereo vision. We also demonstrate the effectiveness of the proposed method by carrying out the real robot manipulation task. PMID:28629139

Prism-based single-camera system for stereo display

NASA Astrophysics Data System (ADS)

Zhao, Yue; Cui, Xiaoyu; Wang, Zhiguo; Chen, Hongsheng; Fan, Heyu; Wu, Teresa

2016-06-01

This paper combines the prism and single camera and puts forward a method of stereo imaging with low cost. First of all, according to the principle of geometrical optics, we can deduce the relationship between the prism single-camera system and dual-camera system, and according to the principle of binocular vision we can deduce the relationship between binoculars and dual camera. Thus we can establish the relationship between the prism single-camera system and binoculars and get the positional relation of prism, camera, and object with the best effect of stereo display. Finally, using the active shutter stereo glasses of NVIDIA Company, we can realize the three-dimensional (3-D) display of the object. The experimental results show that the proposed approach can make use of the prism single-camera system to simulate the various observation manners of eyes. The stereo imaging system, which is designed by the method proposed by this paper, can restore the 3-D shape of the object being photographed factually.

Design and Implementation of a Novel Portable 360° Stereo Camera System with Low-Cost Action Cameras

NASA Astrophysics Data System (ADS)

Holdener, D.; Nebiker, S.; Blaser, S.

2017-11-01

The demand for capturing indoor spaces is rising with the digitalization trend in the construction industry. An efficient solution for measuring challenging indoor environments is mobile mapping. Image-based systems with 360° panoramic coverage allow a rapid data acquisition and can be processed to georeferenced 3D images hosted in cloud-based 3D geoinformation services. For the multiview stereo camera system presented in this paper, a 360° coverage is achieved with a layout consisting of five horizontal stereo image pairs in a circular arrangement. The design is implemented as a low-cost solution based on a 3D printed camera rig and action cameras with fisheye lenses. The fisheye stereo system is successfully calibrated with accuracies sufficient for the applied measurement task. A comparison of 3D distances with reference data delivers maximal deviations of 3 cm on typical distances in indoor space of 2-8 m. Also the automatic computation of coloured point clouds from the stereo pairs is demonstrated.

3D digital image correlation using single color camera pseudo-stereo system

NASA Astrophysics Data System (ADS)

Li, Junrui; Dan, Xizuo; Xu, Wan; Wang, Yonghong; Yang, Guobiao; Yang, Lianxiang

2017-10-01

Three dimensional digital image correlation (3D-DIC) has been widely used by industry to measure the 3D contour and whole-field displacement/strain. In this paper, a novel single color camera 3D-DIC setup, using a reflection-based pseudo-stereo system, is proposed. Compared to the conventional single camera pseudo-stereo system, which splits the CCD sensor into two halves to capture the stereo views, the proposed system achieves both views using the whole CCD chip and without reducing the spatial resolution. In addition, similarly to the conventional 3D-DIC system, the center of the two views stands in the center of the CCD chip, which minimizes the image distortion relative to the conventional pseudo-stereo system. The two overlapped views in the CCD are separated by the color domain, and the standard 3D-DIC algorithm can be utilized directly to perform the evaluation. The system's principle and experimental setup are described in detail, and multiple tests are performed to validate the system.

Analysis of Long Bone and Vertebral Failure Patterns.

DTIC Science & Technology

1982-09-30

processes further supported the findings of • :the scanning electron microscopy studies . In the impacted animals, the cartilage surface was eroded... cartilage matrix. In the six years post-impaction group, the articular cartilage had converted to fibrocartilage instead of normal hyaline cartilage . The...columns of four rhesus monkeys have been collected and are being processed for study with light microscopy and scanning electron microscopy. The baboon

A simple method for maintaining relative positions of separate tissue elements during processing for electron microscopy.

PubMed

Stirling, C A

1978-09-01

Molten (328 K) 20% gelatin is used as a 'glue' to hold together separate tissue elements or tissue elements that may be separated when cutting small blocks of tissue for plastic embedding. Standard aldehyde and osmium fixation, dehydration and epoxy embedding are compatible with this as is semi-thin sectioning for light microscopy or thin sectioning for electron microscopy.

Symposium LL: Nanowires--Synthesis Properties Assembly and Application

DTIC Science & Technology

2010-09-10

dedicated hard x - ray microscopy beamline is operated in partnership with the Advanced Photon Source to provide fluorescence, diffraction, and...characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X - ray diffraction (XRD) measurements, proving it to be...Investigation of Preferred Growth Direction of GaN Nanorods by Synchrotron X - ray Reciprocal Space Mapping. Yuri Sohn1, Sanghwa Lee1, Chinkyo Kim1 and Dong

A Time-of-Flight Method to Measure the Speed of Sound Using a Stereo Sound Card

ERIC Educational Resources Information Center

Carvalho, Carlos C.; dos Santos, J. M. B. Lopes; Marques, M. B.

2008-01-01

Most homes in developed countries have a sophisticated data acquisition board, namely the PC sound board. Designed to be able to reproduce CD-quality stereo sound, it must have a sampling rate of at least 44 kHz and have very accurate timing between the two stereo channels. With a very simple adaptation of a pair of regular PC microphones, a…

STEREO Press Briefing

NASA Image and Video Library

2011-08-17

Madhulika Guhathakurta, seated left, STEREO program scientist, speaks during a press briefing, Thursday, Aug. 18, 2011, at NASA Headquarters in Washington as Craig DeForest, David Webb and Alysha Reinard, look on. The briefing was held to discusses new details about the structure of solar storms and the impact they have on Earth. The new information comes from NASA's Solar Terrestrial Relations Observatory, or STEREO, spacecraft and other NASA probes. Photo Credit: (NASA/GSFC/Rebecca Roth)

STEREO Press Briefing

NASA Image and Video Library

2011-08-17

Madhulika Guhathakurta, seated left, STEREO program scientist, speaks during a press briefing, Thursday, Aug. 18, 2011, at NASA Headquarters in Washington as Craig DeForest, David Webb and Alysha Reinard, look on. The briefing was held to discusses new details about the structure of solar storms and the impact they have on Earth. The new information comes from NASA's Solar Terrestrial Relations Observatory, or STEREO, spacecraft and other NASA probes. Photo Credit: (NASA/GSFC/Rebecca Roth).

Consequences of Incorrect Focus Cues in Stereo Displays

PubMed Central

Banks, Martin S.; Akeley, Kurt; Hoffman, David M.; Girshick, Ahna R.

2010-01-01

Conventional stereo displays produce images in which focus cues – blur and accommodation – are inconsistent with the simulated depth. We have developed new display techniques that allow the presentation of nearly correct focus. Using these techniques, we find that stereo vision is faster and more accurate when focus cues are mostly consistent with simulated depth; furthermore, viewers experience less fatigue when focus cues are correct or nearly correct. PMID:20523910

Evaluation of Deep Learning Based Stereo Matching Methods: from Ground to Aerial Images

NASA Astrophysics Data System (ADS)

Liu, J.; Ji, S.; Zhang, C.; Qin, Z.

2018-05-01

Dense stereo matching has been extensively studied in photogrammetry and computer vision. In this paper we evaluate the application of deep learning based stereo methods, which were raised from 2016 and rapidly spread, on aerial stereos other than ground images that are commonly used in computer vision community. Two popular methods are evaluated. One learns matching cost with a convolutional neural network (known as MC-CNN); the other produces a disparity map in an end-to-end manner by utilizing both geometry and context (known as GC-net). First, we evaluate the performance of the deep learning based methods for aerial stereo images by a direct model reuse. The models pre-trained on KITTI 2012, KITTI 2015 and Driving datasets separately, are directly applied to three aerial datasets. We also give the results of direct training on target aerial datasets. Second, the deep learning based methods are compared to the classic stereo matching method, Semi-Global Matching(SGM), and a photogrammetric software, SURE, on the same aerial datasets. Third, transfer learning strategy is introduced to aerial image matching based on the assumption of a few target samples available for model fine tuning. It experimentally proved that the conventional methods and the deep learning based methods performed similarly, and the latter had greater potential to be explored.

Monocular Stereo Measurement Using High-Speed Catadioptric Tracking

PubMed Central

Hu, Shaopeng; Matsumoto, Yuji; Takaki, Takeshi; Ishii, Idaku

2017-01-01

This paper presents a novel concept of real-time catadioptric stereo tracking using a single ultrafast mirror-drive pan-tilt active vision system that can simultaneously switch between hundreds of different views in a second. By accelerating video-shooting, computation, and actuation at the millisecond-granularity level for time-division multithreaded processing in ultrafast gaze control, the active vision system can function virtually as two or more tracking cameras with different views. It enables a single active vision system to act as virtual left and right pan-tilt cameras that can simultaneously shoot a pair of stereo images for the same object to be observed at arbitrary viewpoints by switching the direction of the mirrors of the active vision system frame by frame. We developed a monocular galvano-mirror-based stereo tracking system that can switch between 500 different views in a second, and it functions as a catadioptric active stereo with left and right pan-tilt tracking cameras that can virtually capture 8-bit color 512×512 images each operating at 250 fps to mechanically track a fast-moving object with a sufficient parallax for accurate 3D measurement. Several tracking experiments for moving objects in 3D space are described to demonstrate the performance of our monocular stereo tracking system. PMID:28792483

3D reconstruction of the optic nerve head using stereo fundus images for computer-aided diagnosis of glaucoma

NASA Astrophysics Data System (ADS)

Tang, Li; Kwon, Young H.; Alward, Wallace L. M.; Greenlee, Emily C.; Lee, Kyungmoo; Garvin, Mona K.; Abràmoff, Michael D.

2010-03-01

The shape of the optic nerve head (ONH) is reconstructed automatically using stereo fundus color images by a robust stereo matching algorithm, which is needed for a quantitative estimate of the amount of nerve fiber loss for patients with glaucoma. Compared to natural scene stereo, fundus images are noisy because of the limits on illumination conditions and imperfections of the optics of the eye, posing challenges to conventional stereo matching approaches. In this paper, multi scale pixel feature vectors which are robust to noise are formulated using a combination of both pixel intensity and gradient features in scale space. Feature vectors associated with potential correspondences are compared with a disparity based matching score. The deep structures of the optic disc are reconstructed with a stack of disparity estimates in scale space. Optical coherence tomography (OCT) data was collected at the same time, and depth information from 3D segmentation was registered with the stereo fundus images to provide the ground truth for performance evaluation. In experiments, the proposed algorithm produces estimates for the shape of the ONH that are close to the OCT based shape, and it shows great potential to help computer-aided diagnosis of glaucoma and other related retinal diseases.

The Sun in STEREO

NASA Technical Reports Server (NTRS)

2006-01-01

Parallax gives depth to life. Simultaneous viewing from slightly different vantage points makes binocular humans superior to monocular cyclopes, and fixes us in the third dimension of the Universe. We've been stunned by 3-d images of Venus and Mars (along with more familiar views of earth). Now astronomers plan to give us the best view of all, 3-d images of the dynamic Sun. That's one of the prime goals of NASA's Solar Terrestrial Relations Observatories, also known as STEREO. STEREO is a pair of spacecraft observatories, one placed in orbit in front of earth, and one to be placed in an earth-trailing orbit. Simultaneous observations of the Sun with the two STEREO spacecraft will provide extraordinary 3-d views of all types of solar activity, especially the dramatic events called coronal mass ejections which send high energy particles from the outer solar atmosphere hurtling towards earth. The image above the first image of the sun by the two STEREO spacecraft, an extreme ultraviolet shot of the Sun's million-degree corona, taken by the Extreme Ultraviolet Imager on the Sun Earth Connection Coronal and Heliospheric Investigation (SECCHI) instrument package. STEREO's first 3-d solar images should be available in April if all goes well. Put on your red and blue glasses!

Neural architectures for stereo vision.

PubMed

Parker, Andrew J; Smith, Jackson E T; Krug, Kristine

2016-06-19

Stereoscopic vision delivers a sense of depth based on binocular information but additionally acts as a mechanism for achieving correspondence between patterns arriving at the left and right eyes. We analyse quantitatively the cortical architecture for stereoscopic vision in two areas of macaque visual cortex. For primary visual cortex V1, the result is consistent with a module that is isotropic in cortical space with a diameter of at least 3 mm in surface extent. This implies that the module for stereo is larger than the repeat distance between ocular dominance columns in V1. By contrast, in the extrastriate cortical area V5/MT, which has a specialized architecture for stereo depth, the module for representation of stereo is about 1 mm in surface extent, so the representation of stereo in V5/MT is more compressed than V1 in terms of neural wiring of the neocortex. The surface extent estimated for stereo in V5/MT is consistent with measurements of its specialized domains for binocular disparity. Within V1, we suggest that long-range horizontal, anatomical connections form functional modules that serve both binocular and monocular pattern recognition: this common function may explain the distortion and disruption of monocular pattern vision observed in amblyopia.This article is part of the themed issue 'Vision in our three-dimensional world'. © 2016 The Authors.

STEM VQ Method, Using Scanning Transmission Electron Microscopy (STEM) for Accurate Virus Quantification

DTIC Science & Technology

2017-02-02

Corresponding Author Abstract Accurate virus quantification is sought, but a perfect method still eludes the scientific community. Electron...unlimited. UNCLASSIFIED 2 provides morphology data and counts all viral particles, including partial or noninfectious particles; however, EM methods ...consistent, reproducible virus quantification method called Scanning Transmission Electron Microscopy – Virus Quantification (STEM-VQ) which simplifies

Evaluation of a hybrid pixel detector for electron microscopy.

PubMed

Faruqi, A R; Cattermole, D M; Henderson, R; Mikulec, B; Raeburn, C

2003-04-01

We describe the application of a silicon hybrid pixel detector, containing 64 by 64 pixels, each 170 microm(2), in electron microscopy. The device offers improved resolution compared to CCDs along with faster and noiseless readout. Evaluation of the detector, carried out on a 120 kV electron microscope, demonstrates the potential of the device.

Mineralogy and Microstructures of Shock-Induced Melt Veins in Chondrites

NASA Technical Reports Server (NTRS)

Sharp, Thomas G.

2000-01-01

The applicability of phase equilibrium data to the interpretation of shock-induced melt veins can only be tested by a detailed study of melt- vein mineralogy to see how high-pressure assemblages vary as a function of shock conditions inferred from other indicators. We have used transmission electron microscopy (TEM), analytical electron microscopy (AEM), scanning electron microscopy (SEM), electron microprobe analysis (EMA) and optical petrography to characterize the mineralogy, microstructures, and compositions of melt veins and associated high-pressure minerals in shocked chondrites and SNC meteorites. In the processes, we have gained a better understanding of what melt veining can tell us about shock conditions and we have discovered new mineral phases in chondritic and SNC meteorites.

Correlative fluorescence and scanning transmission electron microscopy of quantum dot-labeled proteins on whole cells in liquid.

PubMed

Peckys, Diana B; Bandmann, Vera; de Jonge, Niels

2014-01-01

Correlative fluorescence microscopy combined with scanning transmission electron microscopy (STEM) of cells fully immersed in liquid is a new methodology with many application areas. Proteins, in live cells immobilized on microchips, are labeled with fluorescent quantum dot nanoparticles. In this protocol, the epidermal growth factor receptor (EGFR) is labeled. The cells are fixed after a selected labeling time, for example, 5 min as needed to form EGFR dimers. The microchip with cells is then imaged with fluorescence microscopy. Thereafter, STEM can be accomplished in two ways. The microchip with the labeled cells and one microchip with a spacer are assembled into a special microfluidic device and imaged with dedicated high-voltage STEM. Alternatively, thin edges of cells can be studied with environmental scanning electron microscopy with a STEM detector, by placing a microchip with cells in a cooled wet environment. © 2014 Elsevier Inc. All rights reserved.

Schottky barrier height measurements of Cu/Si(001), Ag/Si(001), and Au/Si(001) interfaces utilizing ballistic electron emission microscopy and ballistic hole emission microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Balsano, Robert; Matsubayashi, Akitomo; LaBella, Vincent P., E-mail: vlabella@albany.edu

2013-11-15

The Schottky barrier heights of both n and p doped Cu/Si(001), Ag/Si(001), and Au/Si(001) diodes were measured using ballistic electron emission microscopy and ballistic hole emission microscopy (BHEM), respectively. Measurements using both forward and reverse ballistic electron emission microscopy (BEEM) and (BHEM) injection conditions were performed. The Schottky barrier heights were found by fitting to a linearization of the power law form of the Bell-Kaiser BEEM model. The sum of the n-type and p-type barrier heights are in good agreement with the band gap of silicon and independent of the metal utilized. The Schottky barrier heights are found to bemore » below the region of best fit for the power law form of the BK model, demonstrating its region of validity.« less

Grinding and polishing instead of sectioning for the tissue samples with a graft: Implications for light and electron microscopy.

PubMed

Mukhamadiyarov, Rinat A; Sevostyanova, Victoria V; Shishkova, Daria K; Nokhrin, Andrey V; Sidorova, Olga D; Kutikhin, Anton G

2016-06-01

A broad use of the graft replacement requires a detailed investigation of the host-graft interaction, including both histological examination and electron microscopy. A high quality sectioning of the host tissue with a graft seems to be complicated; in addition, it is difficult to examine the same tissue area by both of the mentioned microscopy techniques. To solve these problems, we developed a new technique of epoxy resin embedding with the further grinding, polishing, and staining. Graft-containing tissues prepared by grinding and polishing preserved their structure; however, sectioning frequently required the explantation of the graft and led to tissue disintegration. Moreover, stained samples prepared by grinding and polishing may then be assessed by both light microscopy and backscattered scanning electron microscopy. Therefore, grinding and polishing outperform sectioning when applied to the tissues with a graft. Copyright © 2016 Elsevier Ltd. All rights reserved.

Ultrastructural characterization of the new NG97ht human-derived glioma cell line using two different electron microscopy technical procedures.

PubMed

Machado, Camila Maria Longo; Zorzeto, Tatiane Queiroz; Bianco, Juares E Romero; Rosa, Renata Giardini; Genari, Selma Candelaria; Joazeiro, Paulo Pinto; Verinaud, Liana

2009-04-01

On the basis of transmission electron microscopy observations in tumor cell lines, oncologists have made innumerous diagnostic and therapeutical progresses. Following this path, the UNICAMP immunopathologies laboratory established the NG97 cell line derived from a human astrocytoma grade III, which when injected to the athymic nude mouse flank developed a grade IV astrocytoma. In this study, we focused on ultrastructural characterization of the NG97 cells after being recovered from xenotransplant (NG97ht). These cells in culture were assayed by two different electron microscopy procedures to characterize ultrastructures related to grade IV astrocytomas and to observe their structures through cell subcultivation. Additionally, comparative morphological descriptions of different cell passages in these technical procedures could be a useful tool for improving electron microscopy cell lineage protocols. Results from many cell passage observations showed ultrastructural similarities, which suggest malignant and glioblastoma phenotypes. In the first procedure, NG97ht cells were harvested and then incorporated into agarose before subjecting them to electron microscopy protocols, whereas in the second one, monolayer cells grew first on cover slides. Comparison among protocols revealed that organelles, cytoplasmatic extensions, spatial conformation of filopodia, and cell attachment to substrate were more preserved in the second procedure. Furthermore, in this latter procedure, a unique ellipsoidal structure was observed, which was already described when dealing with gliosarcoma cell line elsewhere. Therefore, these analyses demonstrated a morphological characterization of a new NG97ht cell line using electron transmission microscopy. Moreover, it has been shown that the second procedure provides more detailed information compared with the first.

Aharonov-Bohm Effect in the Photodetachment Microscopy of Hydrogen Negative Ions in an Electric Field

NASA Astrophysics Data System (ADS)

Wang, Dehua

2014-09-01

The Aharonov-Bohm (AB) effect in the photodetachment microscopy of the H- ions in an electric field has been studied on the basis of the semiclassical theory. After the H- ion is irradiated by a laser light, they provide a coherent electron source. When the detached electron is accelerated by a uniform electric field, two trajectories of a detached electron which run from the source to the same point on the detector, will interfere with each other and lead to an interference pattern in the photodetachment microscopy. After the solenoid is electrified beside the H- ion, even though no Lorentz force acts on the electron outside the solenoid, the photodetachment microscopy interference pattern on the detector is changed with the variation in the magnetic flux enclosed by the solenoid. This is caused by the AB effect. Under certain conditions, the interference pattern reaches the macroscopic dimensions and could be observed in a direct AB effect experiment. Our study can provide some predictions for the future experimental study of the AB effect in the photodetachment microscopy of negative ions.

Analysis of liquid suspensions using scanning electron microscopy in transmission: estimation of the water film thickness using Monte-Carlo simulations.

PubMed

Xiao, J; Foray, G; Masenelli-Varlot, K

2018-02-01

Environmental scanning electron microscopy (ESEM) allows the observation of liquids under specific conditions of pressure and temperature. Moreover, when working in the transmission mode, that is in scanning transmission electron microscopy (STEM), nano-objects can be analysed inside a liquid. The contrast in the images is mass-thickness dependent as in STEM-in-TEM (transmission electron microscopy) using closed cells. However, in STEM-in-ESEM, as the liquid-vapour equilibrium is kept dynamically, the thickness of the water droplet remains unknown. In this paper, the contrasts measured in the experimental images are compared with calculations using Monte-Carlo simulations in order to estimate the thickness of water. Two examples are given. On gold nanoparticles, the thickness of a thick film can be estimated thanks to a contrast inversion. On core-shell latex particles, the grey level of the shell compared with those of the core and of the water film gives a relatively precise measurement of the water film thickness. © 2017 The Authors Journal of Microscopy © 2017 Royal Microscopical Society.

3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

PubMed Central

Lerner, Thomas R.; Burden, Jemima J.; Nkwe, David O.; Pelchen-Matthews, Annegret; Domart, Marie-Charlotte; Durgan, Joanne; Weston, Anne; Jones, Martin L.; Peddie, Christopher J.; Carzaniga, Raffaella; Florey, Oliver; Marsh, Mark; Gutierrez, Maximiliano G.

2017-01-01

ABSTRACT The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastructure. The workflow encompasses all steps from cell culture to sample processing, imaging strategy, and 3D image processing and analysis. We demonstrate successful application of the workflow to three studies, each aiming to better understand complex and dynamic biological processes, including bacterial and viral infections of cultured cells and formation of entotic cell-in-cell structures commonly observed in tumours. Our workflow revealed new insight into the replicative niche of Mycobacterium tuberculosis in primary human lymphatic endothelial cells, HIV-1 in human monocyte-derived macrophages, and the composition of the entotic vacuole. The broad application of this 3D CLEM technique will make it a useful addition to the correlative imaging toolbox for biomedical research. PMID:27445312

Stereo-tomography in triangulated models

NASA Astrophysics Data System (ADS)

Yang, Kai; Shao, Wei-Dong; Xing, Feng-yuan; Xiong, Kai

2018-04-01

Stereo-tomography is a distinctive tomographic method. It is capable of estimating the scatterer position, the local dip of scatterer and the background velocity simultaneously. Building a geologically consistent velocity model is always appealing for applied and earthquake seismologists. Differing from the previous work to incorporate various regularization techniques into the cost function of stereo-tomography, we think extending stereo-tomography to the triangulated model will be the most straightforward way to achieve this goal. In this paper, we provided all the Fréchet derivatives of stereo-tomographic data components with respect to model components for slowness-squared triangulated model (or sloth model) in 2D Cartesian coordinate based on the ray perturbation theory for interfaces. A sloth model representation means a sparser model representation when compared with conventional B-spline model representation. A sparser model representation leads to a smaller scale of stereo-tomographic (Fréchet) matrix, a higher-accuracy solution when solving linear equations, a faster convergence rate and a lower requirement for quantity of data space. Moreover, a quantitative representation of interface strengthens the relationships among different model components, which makes the cross regularizations among these model components, such as node coordinates, scatterer coordinates and scattering angles, etc., more straightforward and easier to be implemented. The sensitivity analysis, the model resolution matrix analysis and a series of synthetic data examples demonstrate the correctness of the Fréchet derivatives, the applicability of the regularization terms and the robustness of the stereo-tomography in triangulated model. It provides a solid theoretical foundation for the real applications in the future.

Simulated electron beam trajectories toward a field ion microscopy specimen

NASA Astrophysics Data System (ADS)

Larson, D. J.; Camus, P. P.; Kelly, T. F.

1993-04-01

This article explores the conditions under which a directed electron beam originating nearly normal to the specimen axis can be made to impact the near-apex region of a field ion microscopy specimen in a high electric field. Electron trajectories were calculated using a modified Runge-Kutta numerical method. The results indicate that an electron beam can be directed to a specimen under typical field ion microscopy conditions using two methods: by varying initial beam tilt (less than 60 mrad) or by translating the initial beam position relative to the specimen apex (less than 5 mm). The net focusing effect of the high electric field on the electron beam can be treated, to first order, as an astigmatism and may be correctable by a post-lens deflection system.

Direct observation of iron-induced conformational changes of mitochondrial DNA by high-resolution field-emission in-lens scanning electron microscopy.

PubMed Central

Yaffee, M; Walter, P; Richter, C; Müller, M

1996-01-01

When respiring rat liver mitochondria are incubated in the presence of Fe(III) gluconate, their DNA (mtDNA) relaxes from the supercoiled to the open circular form dependent on the iron dose. Anaerobiosis or antioxidants fail to completely inhibit the unwinding. High-resolution field-emission in-lens scanning electron microscopy imaging, in concert with backscattered electron detection, pinpoints nanometer-range iron colloids bound to mtDNA isolated from iron-exposed mitochondria. High-resolution field-emission in-lens scanning electron microscopy with backscattered electron detection imaging permits simultaneous detailed visual analysis of DNA topology, iron dose-dependent mtDNA unwinding, and assessment of iron colloid formation on mtDNA strands. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:8643576

CMEs in the Heliosphere: I. A Statistical Analysis of the Observational Properties of CMEs Detected in the Heliosphere from 2007 to 2017 by STEREO/HI-1

NASA Astrophysics Data System (ADS)

Harrison, R. A.; Davies, J. A.; Barnes, D.; Byrne, J. P.; Perry, C. H.; Bothmer, V.; Eastwood, J. P.; Gallagher, P. T.; Kilpua, E. K. J.; Möstl, C.; Rodriguez, L.; Rouillard, A. P.; Odstrčil, D.

2018-05-01

We present a statistical analysis of coronal mass ejections (CMEs) imaged by the Heliospheric Imager (HI) instruments on board NASA's twin-spacecraft STEREO mission between April 2007 and August 2017 for STEREO-A and between April 2007 and September 2014 for STEREO-B. The analysis exploits a catalogue that was generated within the FP7 HELCATS project. Here, we focus on the observational characteristics of CMEs imaged in the heliosphere by the inner (HI-1) cameras, while following papers will present analyses of CME propagation through the entire HI fields of view. More specifically, in this paper we present distributions of the basic observational parameters - namely occurrence frequency, central position angle (PA) and PA span - derived from nearly 2000 detections of CMEs in the heliosphere by HI-1 on STEREO-A or STEREO-B from the minimum between Solar Cycles 23 and 24 to the maximum of Cycle 24; STEREO-A analysis includes a further 158 CME detections from the descending phase of Cycle 24, by which time communication with STEREO-B had been lost. We compare heliospheric CME characteristics with properties of CMEs observed at coronal altitudes, and with sunspot number. As expected, heliospheric CME rates correlate with sunspot number, and are not inconsistent with coronal rates once instrumental factors/differences in cataloguing philosophy are considered. As well as being more abundant, heliospheric CMEs, like their coronal counterparts, tend to be wider during solar maximum. Our results confirm previous coronagraph analyses suggesting that CME launch sites do not simply migrate to higher latitudes with increasing solar activity. At solar minimum, CMEs tend to be launched from equatorial latitudes, while at maximum, CMEs appear to be launched over a much wider latitude range; this has implications for understanding the CME/solar source association. Our analysis provides some supporting evidence for the systematic dragging of CMEs to lower latitude as they propagate outwards.

Accuracy analysis for DSM and orthoimages derived from SPOT HRS stereo data using direct georeferencing

NASA Astrophysics Data System (ADS)

Reinartz, Peter; Müller, Rupert; Lehner, Manfred; Schroeder, Manfred

During the HRS (High Resolution Stereo) Scientific Assessment Program the French space agency CNES delivered data sets from the HRS camera system with high precision ancillary data. Two test data sets from this program were evaluated: one is located in Germany, the other in Spain. The first goal was to derive orthoimages and digital surface models (DSM) from the along track stereo data by applying the rigorous model with direct georeferencing and without ground control points (GCPs). For the derivation of DSM, the stereo processing software, developed at DLR for the MOMS-2P three line stereo camera was used. As a first step, the interior and exterior orientation of the camera, delivered as ancillary data from positioning and attitude systems were extracted. A dense image matching, using nearly all pixels as kernel centers provided the parallaxes. The quality of the stereo tie points was controlled by forward and backward matching of the two stereo partners using the local least squares matching method. Forward intersection lead to points in object space which are subsequently interpolated to a DSM in a regular grid. DEM filtering methods were also applied and evaluations carried out differentiating between accuracies in forest and other areas. Additionally, orthoimages were generated from the images of the two stereo looking directions. The orthoimage and DSM accuracy was determined by using GCPs and available reference DEMs of superior accuracy (DEM derived from laser data and/or classical airborne photogrammetry). As expected the results obtained without using GCPs showed a bias in the order of 5-20 m to the reference data for all three coordinates. By image matching it could be shown that the two independently derived orthoimages exhibit a very constant shift behavior. In a second step few GCPs (3-4) were used to calculate boresight alignment angles, introduced into the direct georeferencing process of each image independently. This method improved the absolute accuracy of the resulting orthoimages and DSM significantly.

STEREO-IMPACT Education and Public Outreach: Sharing STEREO Science

NASA Astrophysics Data System (ADS)

Craig, N.; Peticolas, L. M.; Mendez, B. J.

2005-12-01

The Solar TErrestrial RElations Observatory (STEREO) is scheduled for launch in Spring 2006. STEREO will study the Sun with two spacecrafts in orbit around it and on either side of Earth. The primary science goal is to understand the nature and consequences of Coronal Mass Ejections (CMEs). Despite their importance, scientists don't fully understand the origin and evolution of CMEs, nor their structure or extent in interplanetary space. STEREO's unique 3-D images of the structure of CMEs will enable scientists to determine their fundamental nature and origin. We will discuss the Education and Public Outreach (E/PO) program for the In-situ Measurement of Particles And CME Transients (IMPACT) suite of instruments aboard the two crafts and give examples of upcoming activities, including NASA's Sun-Earth day events, which are scheduled to coincide with a total solar eclipse in March. This event offers a good opportunity to engage the public in STEREO science, because an eclipse allows one to see the solar corona from where CMEs erupt. STEREO's connection to space weather lends itself to close partnerships with the Sun-Earth Connection Education Forum (SECEF), The Exploratorium, and UC Berkeley's Center for New Music and Audio Technologies to develop informal science programs for science centers, museum visitors, and the public in general. We will also discuss our teacher workshops locally in California and also at annual conferences such as those of the National Science Teachers Association. Such workshops often focus on magnetism and its connection to CMEs and Earth's magnetic field, leading to the questions STEREO scientists hope to answer. The importance of partnerships and coordination in working in an instrument E/PO program that is part of a bigger NASA mission with many instrument suites and many PIs will be emphasized. The Education and Outreach Porgram is funded by NASA's SMD.

Reprint of: Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film.

PubMed

Nishiyama, Hidetoshi; Suga, Mitsuo; Ogura, Toshihiko; Maruyama, Yuusuke; Koizumi, Mitsuru; Mio, Kazuhiro; Kitamura, Shinichi; Sato, Chikara

2010-11-01

Direct observation of subcellular structures and their characterization is essential for understanding their physiological functions. To observe them in open environment, we have developed an inverted scanning electron microscope with a detachable, open-culture dish, capable of 8 nm resolution, and combined with a fluorescence microscope quasi-simultaneously observing the same area from the top. For scanning electron microscopy from the bottom, a silicon nitride film window in the base of the dish maintains a vacuum between electron gun and open sample dish while allowing electrons to pass through. Electrons are backscattered from the sample and captured by a detector under the dish. Cells cultured on the open dish can be externally manipulated under optical microscopy, fixed, and observed using scanning electron microscopy. Once fine structures have been revealed by scanning electron microscopy, their component proteins may be identified by comparison with separately prepared fluorescence-labeled optical microscopic images of the candidate proteins, with their heavy-metal-labeled or stained ASEM images. Furthermore, cell nuclei in a tissue block stained with platinum-blue were successfully observed without thin-sectioning, which suggests the applicability of this inverted scanning electron microscope to cancer diagnosis. This microscope visualizes mesoscopic-scale structures, and is also applicable to non-bioscience fields including polymer chemistry. Copyright © 2010 Elsevier Inc. All rights reserved.

Precision 3d Surface Reconstruction from Lro Nac Images Using Semi-Global Matching with Coupled Epipolar Rectification

NASA Astrophysics Data System (ADS)

Hu, H.; Wu, B.

2017-07-01

The Narrow-Angle Camera (NAC) on board the Lunar Reconnaissance Orbiter (LRO) comprises of a pair of closely attached high-resolution push-broom sensors, in order to improve the swath coverage. However, the two image sensors do not share the same lenses and cannot be modelled geometrically using a single physical model. Thus, previous works on dense matching of stereo pairs of NAC images would generally create two to four stereo models, each with an irregular and overlapping region of varying size. Semi-Global Matching (SGM) is a well-known dense matching method and has been widely used for image-based 3D surface reconstruction. SGM is a global matching algorithm relying on global inference in a larger context rather than individual pixels to establish stable correspondences. The stereo configuration of LRO NAC images causes severe problem for image matching methods such as SGM, which emphasizes global matching strategy. Aiming at using SGM for image matching of LRO NAC stereo pairs for precision 3D surface reconstruction, this paper presents a coupled epipolar rectification methods for LRO NAC stereo images, which merges the image pair in the disparity space and in this way, only one stereo model will be estimated. For a stereo pair (four) of NAC images, the method starts with the boresight calibration by finding correspondence in the small overlapping stripe between each pair of NAC images and bundle adjustment of the stereo pair, in order to clean the vertical disparities. Then, the dominate direction of the images are estimated by project the center of the coverage area to the reference image and back-projected to the bounding box plane determined by the image orientation parameters iteratively. The dominate direction will determine an affine model, by which the pair of NAC images are warped onto the object space with a given ground resolution and in the meantime, a mask is produced indicating the owner of each pixel. SGM is then used to generate a disparity map for the stereo pair and each correspondence is transformed back to the owner and 3D points are derived through photogrammetric space intersection. Experimental results reveal that the proposed method is able to reduce gaps and inconsistencies caused by the inaccurate boresight offsets between the two NAC cameras and the irregular overlapping regions, and finally generate precise and consistent 3D surface models from the NAC stereo images automatically.

Role of stereoscopic imaging in the astronomical study of nearby stars and planetary systems

NASA Astrophysics Data System (ADS)

Mark, David S.; Waste, Corby

1997-05-01

The development of stereoscopic imaging as a 3D spatial mapping tool for planetary science is now beginning to find greater usefulness in the study of stellar atmospheres and planetary systems in general. For the first time, telescopes and accompanying spectrometers have demonstrated the capacity to depict the gyrating motion of nearby stars so precisely as to derive the existence of closely orbiting Jovian-type planets, which are gravitationally influencing the motion of the parent star. Also for the first time, remote space borne telescopes, unhindered by atmospheric effects, are recording and tracking the rotational characteristics of our nearby star, the sun, so accurately as to reveal and identify in great detail the heightened turbulence of the sun's corona. In order to perform new forms of stereo imaging and 3D reconstruction with such large scale objects as stars and planets, within solar systems, a set of geometrical parameters must be observed, and are illustrated here. The behavior of nearby stars can be studied over time using an astrometric approach, making use of the earth's orbital path as a semi- yearly stereo base for the viewing telescope. As is often the case in this method, the resulting stereo angle becomes too narrow to afford a beneficial stereo view, given the star's distance and the general level of detected noise in the signal. With the advent, though, of new earth based and space borne interferometers, operating within various wavelengths including IR, the capability of detecting and assembling the full 3-dimensional axes of motion of nearby gyrating stars can be achieved. In addition, the coupling of large interferometers with combined data sets can provide large stereo bases and low signal noise to produce converging 3- dimensional stereo views of nearby planetary systems. Several groups of new astronomical stereo imaging data sets are presented, including 3D views of the sun taken by the Solar and Heliospheric Observatory, coincident stereo views of the planet Jupiter during impact of comet Shoemaker-Levy 9, taken by the Galileo spacecraft and the Hubble Space Telescope, as well as views of nearby stars. Spatial ambiguities arising in singular 2-dimensional viewpoints are shown to be resolvable in twin perspective, 3-dimensional stereo views. Stereo imaging of this nature, therefore, occupies a complementary role in astronomical observing, provided the proper fields of view correspond with the path of the orbital geometry of the observing telescope.

Transmission electron microscopy of amyloid fibrils.

PubMed

Gras, Sally L; Waddington, Lynne J; Goldie, Kenneth N

2011-01-01

Transmission Electron Microscopy of negatively stained and cryo-prepared specimens allows amyloid fibrils to be visualised at high resolution in a dried or a hydrated state, and is an essential method for characterising the morphology of fibrils and pre-fibrillar species. We outline the key steps involved in the preparation and observation of samples using negative staining and cryo-electron preservation. We also discuss methods to measure fibril characteristics, such as fibril width, from electron micrographs.

Accurate Virus Quantitation Using a Scanning Transmission Electron Microscopy (STEM) Detector in a Scanning Electron Microscope

DTIC Science & Technology

2017-06-29

Accurate Virus Quantitation Using a Scanning Transmission Electron Microscopy (STEM) Detector in a Scanning Electron Microscope Candace D Blancett1...L Norris2, Cynthia A Rossi4 , Pamela J Glass3, Mei G Sun1,* 1 Pathology Division, United States Army Medical Research Institute of Infectious...Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, Maryland, 21702 2Biostatistics Division, United States Army Medical Research Institute of

Preface: phys. stat. sol. (a) 202/12

NASA Astrophysics Data System (ADS)

Neumann, Wolfgang; Stutzmann, Martin; Hildebrandt, Stefan

2005-09-01

The present special issue contains a collection of Original Papers dedicated to Professor Johannes Heydenreich on the occasion of his 75th birthday.Johannes Heydenreich, born on 20 June 1930 in Plauen/Vogtland near Dresden, studied physics at the Pädagogische Hochschule Potsdam, where he obtained his first academic degree Dipl. Phys. in 1958. He received his doctoral degree at the Martin Luther University in Halle in 1961 and the Habilitation degree in 1969. Already during his studies in Potsdam, he showed an interest in electron microscopy due to the influence of his teacher and supervisor Prof. Picht, one of the pioneers in electron optics. His interests were strengthened when Johannes Heydenreich did the experimental work for his Diploma degree at the Institute for Experimental Physics of the University of Halle, where he met Prof. Heinz Bethge for the first time. This was the beginning of a fruitful and longstanding collaboration. In 1962 Johannes Heydenreich joined the team of the later Institute for Solid State Physics and Electron Microscopy of the Academy of Sciences of the GDR, in Halle, for which the basis was laid by Prof. Bethge in 1960.Heydenreich has been working as Assistant Director for many years and played a decisive role in introducing and organising the various techniques of electron microscopy in the institute.The research activities of Prof. Heydenreich covered a broad spectrum over the years. At the beginning of his career he made significant contributions in the field of electron mirror microscopy. After that, his main interests were focused on transmission electron microscopy, ranging from diffraction contrast analysis of crystal defects to high-resolution electron microscopy and image processing. His favourite field was studies of defect-induced phenomena in advanced materials. The so-called Bethge-Heydenreich, the book Electron Microscopy in Solid State Physics, published at first in a German edition in 1982 and later in a revised English edition by Elsevier in 1987, provides an excellent overview both of Heydenreich's work and of the spectrum of the Institute of Solid State Physics and Electron Micros-copy in Halle.The international reputation of this institute was the basis for the transformation of it, after the re-unification of Germany, into the Max Planck Institute of Microstructure Physics, the first institute of the Max Planck Society in the Eastern part of Germany. From the beginning, Prof. Heydenreich was on the Board of Directors and served as the Executive Director from 1993 until his retirement in 1995.During this year, the International Centre of Advanced Materials and Electron Microscopy celebrates its 30th anniversary. The Centre was founded in Halle in 1975 as a Centre of Electron Microscopy for the Eastern countries.A remarkable and time-consuming part of Heydenreich's work was associated with this centre. Young scientists from Eastern Europe were trained in the theoretical and practical aspects of electron microscopy during the annual spring and autumn meetings. All in all, Johannes Heydenreich conducted 35 of these schools! From the beginning, he was a member of the Scientific Council of the Centre, and from 1985 until his retirement he was the Director of the Centre. Nowadays, the Centre acts as a real bridge between East and West largely due to the efforts of Johannes Heydenreich.Furthermore, Johannes Heydenreich had been active as a leading member of various scientific boards for many years. He was a co-editor of several journals covering electron microscopy, solid state physics and crystallography. As a member of the Executive Committee of the European Society for Elec-tron Microscopy (CESEM) for many years, and as a member of the Executive board of the German Soci-ety for Electron Microscopy, Johannes Heydenreich has invested a great deal of time and effort in the welfare of the scientific community.Over the course of nearly three decades, Johannes Heydenreich worked as a Professor at the Universities of Halle and Leipzig. Generations of students have admired his excellent lectures with clear repre-sentation and rigorous scholarship.The scientific work of Johannes Heydenreich was honoured by his election to the Academy of Natural Scientists Leopoldina where he worked as a Secretary of Natural Science for many years. The Technical University of Chemnitz conferred an honory doctors degree on Prof. Heydenreich in recognition of his scientific work. The German Society for Electron Microscopy awarded him Honorary Membership.Wolfgang Neumann especially acknowledges with gratitude the time of inspiring collaboration in Halle.All his friends, colleagues, and students wish him many further years of good health. The Editors of physica status solidi join these wishes and add their gratefulness for his long-standing and continuous engagement as a member of the Advisory and Editorial Board of physica status solidi (a).

Electron Microscopy Localization and Characterization of Functionalized Composite Organic-Inorganic SERS Nanoparticles on Leukemia Cells

PubMed Central

Koh, Ai Leen; Shachaf, Catherine M.; Elchuri, Sailaja; Nolan, Garry P.; Sinclair, Robert

2008-01-01

We demonstrate the use of electron microscopy as a powerful characterization tool to identify and locate antibody-conjugated composite organic-inorganic (COINs) surface enhanced Raman scattering (SERS) nanoparticles on cells. U937 leukemia cells labeled with antibody CD54-conjugated COINs were characterized in their native, hydrated state using wet Scanning Electron Microscopy (SEM) and in their dehydrated state using high-resolution SEM. In both cases, the backscattered electron detector (BSE) was used to detect and identify the silver constituents in COINs due to its high sensitivity to atomic number variations within a specimen. The imaging and analytical capabilities in the SEM were further complemented by higher resolution Transmission Electron Microscope (TEM) images and Scanning Auger Electron Spectroscopy (AES) data to give reliable and high-resolution information about nanoparticles and their binding to cell surface antigens. PMID:18995965

Evaluations of carbon nanotube field emitters for electron microscopy

NASA Astrophysics Data System (ADS)

Nakahara, Hitoshi; Kusano, Yoshikazu; Kono, Takumi; Saito, Yahachi

2009-11-01

Brightness of carbon nanotube (CNT) emitters was already reported elsewhere. However, brightness of electron emitter is affected by a virtual source size of the emitter, which strongly depends on electron optical configuration around the emitter. In this work, I- V characteristics and brightness of a CNT emitter are measured under a practical field emission electron gun (e-gun) configuration to investigate availability of CNT for electron microscopy. As a result, it is obtained that an emission area of MWNT is smaller than its tip surface area, and the emission area corresponds to a five-membered-ring with 2nd nearest six-membered-rings on the MWNT cap surface. Reduced brightness of MWNT is measured as at least 2.6×109 A/m 2 sr V. It is concluded that even a thick MWNT has enough brightness under a practical e-gun electrode configuration and suitable for electron microscopy.

The application of scanning electron microscopy to fractography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brooks, C.R.; McGill, B.L.

1994-10-01

Many failures involve fracture, and determination of the fracture process is a key factor in understanding the failure. This is frequently accomplished by characterizing the topography of the fracture surface. Scanning electron microscopy has a prominent role in fractography due to three features of the scanning electron microscope (SEM): high resolution, great depth of field, and the ability to obtain chemical information via analysis of the X-rays generated by the electrons. A qualitative treatment is presented of the interaction of electrons with a sample and the effect of the SEM operating parameters on image formation, quality, and X-ray analysis. Fractographsmore » are presented to illustrate these features of scanning electron microscopy and to illustrate the limitations and precautions in obtaining fractographs and x-ray analyses. The review is concluded with examples of fracture surface features of metallic, ceramic, and polymeric materials.« less

The spatial coherence function in scanning transmission electron microscopy and spectroscopy.

PubMed

Nguyen, D T; Findlay, S D; Etheridge, J

2014-11-01

We investigate the implications of the form of the spatial coherence function, also referred to as the effective source distribution, for quantitative analysis in scanning transmission electron microscopy, and in particular for interpreting the spatial origin of imaging and spectroscopy signals. These questions are explored using three different source distribution models applied to a GaAs crystal case study. The shape of the effective source distribution was found to have a strong influence not only on the scanning transmission electron microscopy (STEM) image contrast, but also on the distribution of the scattered electron wavefield and hence on the spatial origin of the detected electron intensities. The implications this has for measuring structure, composition and bonding at atomic resolution via annular dark field, X-ray and electron energy loss STEM imaging are discussed. Copyright © 2014 Elsevier B.V. All rights reserved.