Isolation and characterization of a novel gene sfig in rat skeletal muscle up-regulated by spaceflight (STS-90)

NASA Technical Reports Server (NTRS)

Kano, Mihoko; Kitano, Takako; Ikemoto, Madoka; Hirasaka, Katsuya; Asanoma, Yuki; Ogawa, Takayuki; Takeda, Shinichi; Nonaka, Ikuya; Adams, Gregory R.; Baldwin, Kenneth M.;

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera[W

PubMed Central

Höll, Janine; Vannozzi, Alessandro; Czemmel, Stefan; D'Onofrio, Claudio; Walker, Amanda R.; Rausch, Thomas; Lucchin, Margherita; Boss, Paul K.; Dry, Ian B.; Bogs, Jochen

2013-01-01

Plant stilbenes are phytoalexins that accumulate in a small number of plant species, including grapevine (Vitis vinifera), in response to biotic and abiotic stresses and have been implicated in many beneficial effects on human health. In particular, resveratrol, the basic unit of all other complex stilbenes, has received widespread attention because of its cardio-protective, anticarcinogenic, and antioxidant properties. Although stilbene synthases (STSs), the key enzymes responsible for resveratrol biosynthesis, have been isolated and characterized from several plant species, the transcriptional regulation underlying stilbene biosynthesis is unknown. Here, we report the identification and functional characterization of two R2R3-MYB–type transcription factors (TFs) from grapevine, which regulate the stilbene biosynthetic pathway. These TFs, designated MYB14 and MYB15, strongly coexpress with STS genes, both in leaf tissues under biotic and abiotic stress and in the skin and seed of healthy developing berries during maturation. In transient gene reporter assays, MYB14 and MYB15 were demonstrated to specifically activate the promoters of STS genes, and the ectopic expression of MYB15 in grapevine hairy roots resulted in increased STS expression and in the accumulation of glycosylated stilbenes in planta. These results demonstrate the involvement of MYB14 and MYB15 in the transcriptional regulation of stilbene biosynthesis in grapevine. PMID:24151295

Spaceflight and the Mouse Eye: Results from Experiments on Shuttle Missions STS-133 and STS-135

NASA Technical Reports Server (NTRS)

Zanello, Susana B.; Theriot, Corey A.; Ponce, Claudia Prospero; Chevez-Barrios, Patricia

2013-01-01

Vision alterations associated with globe flattening, chorodial folds and papilledema, shown in some crew members returning from long duration missions. Hypothesis: Ocular neuroanatomical changes observed in the VIIP syndrome are accompanied by retinal changes at the molecular and cellular level that may affect retinal health and physiology. Objective: Investigate evidence of ocular (retinal) changes associated with spaceflight: (1) histological markers of cellular death and damage (2) molecular markers of oxidative stress (3) gene expression markers of stress

Effects of spaceflight on the muscles of the murine shoulder.

PubMed

Shen, Hua; Lim, Chanteak; Schwartz, Andrea G; Andreev-Andrievskiy, Alexander; Deymier, Alix C; Thomopoulos, Stavros

2017-12-01

Mechanical loading is necessary for the development and maintenance of the musculoskeletal system. Removal of loading via microgravity, paralysis, or bed rest leads to rapid loss of muscle mass and function; however, the molecular mechanisms that lead to these changes are largely unknown, particularly for the spaceflight (SF) microgravity environment. Furthermore, few studies have explored these effects on the shoulder, a dynamically stabilized joint with a large range of motion; therefore, we examined the effects of microgravity on mouse shoulder muscles for the 15-d Space Transportation System (STS)-131, 13-d STS-135, and 30-d Bion-M1 missions. Mice from STS missions were euthanized within 4 h after landing, whereas mice from the Bion-M1 mission were euthanized within 14 h after landing. The motion-generating deltoid muscle was more sensitive to microgravity than the joint-stabilizing rotator cuff muscles. Mice from the STS-131 mission exhibited reduced myogenic ( Myf5 and -6 ) and adipogenic ( Pparg , Cebpa , and Lep ) gene expression, whereas either no change or an increased expression of these genes was observed in mice from the Bion-M1 mission. In summary, muscle responses to microgravity were muscle-type specific, short-duration SF caused dramatic molecular changes to shoulder muscles and responses to reloading upon landing were rapid.-Shen, H., Lim, C., Schwartz, A. G., Andreev-Andrievskiy, A., Deymier, A. C., Thomopoulos, S. Effects of spaceflight on the muscles of the murine shoulder. © FASEB.

Comparative analysis of the virulence characteristics of epidemic methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from Chinese children: ST59 MRSA highly expresses core gene-encoded toxin.

PubMed

Li, Shipeng; Sun, Jing; Zhang, Jianzhong; Li, Xiangmei; Tao, Xiaoxia; Wang, Lijuan; Sun, Mingjiao; Liu, Yingchao; Li, Juan; Qiao, Yanhong; Yu, Sangjie; Yao, Kaihu; Yang, Yonghong; Shen, Xuzhuang

2014-02-01

This study aims to investigate the prevalence of a novel cell wall-anchored protein gene, sasX, and to obtain information on the genetic basis for the pathogenic potential of the MRSA strains isolated from Chinese children. The molecular and virulence characteristics of the clinical strains were analyzed. Twenty-two sequence types (STs) were obtained, with six epidemic clones ST59, ST239, ST1, ST910, ST88, and ST338 accounting for 35.8, 22, 6.6, 6.6, 5.3, and 4.1% respectively. The expression levels of hla, psmα, and RNAIII were higher in ST59 than in other STs (p < 0.05). The sasX gene was detected in 26 (10.7%) MRSA isolates. ST239-MRSA-SCCmecIII-t037 (61.5%) was the predominant sasX-positive MRSA clone. The expressions of PSMα and RNAIII were higher in sasX-positive ST239 isolates than in sasX-negative ST239 ones (p < 0.01). Notably, the percentage of invasive infection in infections caused by sasX-positive ST239 MRSA was higher than that by sasX-negative ST239 MRSA (p = 0.008). This study indicated that ST59 was the predominant clone in the MRSA isolates obtained from Chinese children and might have stronger pathogenic potential. The prevalence of the sasX gene in the MRSA isolates from children was relatively low. Furthermore, the sasX gene might be related to the expressions of PSMα and RNAIII and infection invasiveness. © 2013 APMIS Published by John Wiley & Sons Ltd.

Arabidopsis gene expression patterns during spaceflight

NASA Astrophysics Data System (ADS)

Paul, A.-L.; Ferl, R. J.

The exposure of Arabidopsis thaliana (Arabidopsis) plants to spaceflight environments resulted in the differential expression of hundreds of genes. A 5 day mission on orbiter Columbia in 1999 (STS-93) carried transgenic Arabidopsis plants engineered with a transgene composed of the alcohol dehydrogenase (Adh) gene promoter linked to the β -Glucuronidase (GUS) reporter gene. The plants were used to evaluate the effects of spaceflight on two fronts. First, expression patterns visualized with the Adh/GUS transgene were used to address specifically the possibility that spaceflight induces a hypoxic stress response, and to assess whether any spaceflight response was similar to control terrestrial hypoxia-induced gene expression patterns. (Paul et al., Plant Physiol. 2001, 126:613). Second, genome-wide patterns of native gene expression were evaluated utilizing the Affymetrix ATH1 GeneChip? array of 8,000 Arabidopsis genes. As a control for the veracity of the array analyses, a selection of genes identified with the arrays was further characterized with quantitative Real-Time RT PCR (ABI - TaqmanTM). Comparison of the patterns of expression for arrays of hybridized with RNA isolated from plants exposed to spaceflight compared to the control arrays revealed hundreds of genes that were differentially expressed in response to spaceflight, yet most genes that are hallmarks of hypoxic stress were unaffected. These results will be discussed in light of current models for plant responses to the spaceflight environment, and with regard to potential future flight opportunities.

Effects Of Gravitational Perturbation On The Expression Of Genes Regulating Metabolism In Jurkat Cells

PubMed Central

Singh, Kanika; Cubano, Luis; Lewis, Marian

2015-01-01

Gravitational perturbation altered gene expression and increased glucose consumption in spaceflown Jurkat cells. The purpose of this study was to determine if the acceleration experienced during launch was responsible for these changes. In ground-based studies, cells were subjected to typical launch centrifugal acceleration (3g of force for eight minutes) and centrifugal force of 90g for five minutes (commonly used to sediment cells) in a laboratory centrifuge. Controls consisted of static cultures. Gene expression was analyzed by RT-PCR. pH and glucose concentrations were evaluated to monitor metabolic changes. Comparison with controls indicated no significant change in pH or glucose use. Gene expression of Jurkat cells subjected to 3g or 90g of force was altered for only two genes out of seven tested. This research suggests that the changes observed in Jurkat cells flown on STS-95 were not a result of launch acceleration but to other conditions experienced during space flight. PMID:23875517

Primary Culture of Undifferentiated Pleomorphic Sarcoma: Molecular Characterization and Response to Anticancer Agents

PubMed Central

Recine, Federica; Mercatali, Laura; Miserocchi, Giacomo; Spadazzi, Chiara; Liverani, Chiara; Bongiovanni, Alberto; Pieri, Federica; Casadei, Roberto; Riva, Nada; Fausti, Valentina; Amadori, Dino; Ibrahim, Toni

2017-01-01

Undifferentiated pleomorphic sarcoma (UPS) is an aggressive mesenchymal neoplasm with no specific line of differentiation. Eribulin, a novel synthetic microtubule inhibitor, has shown anticancer activity in several tumors, including soft tissue sarcomas (STS). We investigated the molecular biology of UPS, and the mechanisms of action of this innovative microtubule-depolymerizing drug. A primary culture from a patient with UPS was established and characterized in terms of gene expression. The activity of eribulin was also compared with that of other drugs currently used for STS treatment, including trabectedin. Finally, Western blot analysis was performed to better elucidate the activity of eribulin. Our results showed an upregulation of epithelial mesenchymal transition-related genes, and a downregulation of epithelial markers. Furthermore, genes involved in chemoresistance were upregulated. Pharmacological analysis confirmed limited sensitivity to chemotherapy. Interestingly, eribulin exhibited a similar activity to that of standard treatments. Molecular analysis revealed the expression of cell cycle arrest-related and pro-apoptotic-related proteins. These findings are suggestive of aggressive behavior in UPS. Furthermore, the identification of chemoresistance-related genes could facilitate the development of innovative drugs to improve patient outcome. Overall, the results from the present study furnish a rationale for elucidating the role of eribulin for the treatment of UPS. PMID:29292724

Enhanced production of resveratrol derivatives in tobacco plants by improving the metabolic flux of intermediates in the phenylpropanoid pathway.

PubMed

Jeong, Yu Jeong; An, Chul Han; Woo, Su Gyeong; Park, Ji Hye; Lee, Ki-Won; Lee, Sang-Hoon; Rim, Yeonggil; Jeong, Hyung Jae; Ryu, Young Bae; Kim, Cha Young

2016-09-01

The biosynthesis of flavonoids such as anthocyanin and stilbenes has attracted increasing attention because of their potential health benefits. Anthocyanins and stilbenes share common phenylpropanoid precursor pathways. We previously reported that the overexpression of sweetpotato IbMYB1a induced anthocyanin pigmentation in transgenic tobacco (Nicotiana tabacum) plants. In the present study, transgenic tobacco (Nicotiana tabacum SR1) plants (STS-OX and ROST-OX) expressing the RpSTS gene encoding stilbene synthase from rhubarb (Rheum palmatum L. cv. Jangyeop) and the RpSTS and VrROMT genes encoding resveratrol O-methyltransferase from frost grape (Vitis riparia) were generated under the control of 35S promoter. Phenotypic alterations in floral organs, such as a reduction in floral pigments and male sterility, were observed in STS-OX transgenic tobacco plants. However, we failed to obtain STS-OX and ROST-OX plants with high levels of resveratrol compounds. Therefore, to improve the production of resveratrol derivatives in plants, we cross-pollinated flowers of STS-OX or ROST-OX and IbMYB1a-OX transgenic lines (SM and RSM). Phenotypic changes in vegetative and reproductive development of SM and RSM plants were observed. Furthermore, by HPLC and LC-MS analyses, we found enhanced production of resveratrol derivatives such as piceid, piceid methyl ether, resveratrol methyl ether O-hexoside, and 5-methyl resveratrol-3,4'-O-β-D-diglucopyranoside in SM and RSM cross-pollinated lines. Here, total contents of trans- and cis-piceids ranged from approximately 104-240 µg/g fresh weight in SM (F2). Collectively, we suggest that coexpression of RpSTS and IbMYB1a via cross-pollination can induce enhanced production of resveratrol compounds in plants by increasing metabolic flux into stilbenoid biosynthesis.

Expression of estrogenicity genes in a lineage cell culture model of human breast cancer progression

PubMed Central

Fu, Jiaqi; Weise, Amy M.; Falany, Josie L.; Falany, Charles N.; Thibodeau, Bryan J.; Miller, Fred R.; Kocarek, Thomas A.

2013-01-01

TaqMan Gene Expression assays were used to profile the mRNA expression of estrogen receptor (ERα and ERβ) and estrogen metabolism enzymes including cytosolic sulfotransferases (SULT1E1, SULT1A1, SULT2A1, and SULT2B1), steroid sulfatase (STS), aromatase (CYP19), 17β-hydroxysteroid dehydrogenases (17βHSD1 and 2), CYP1B1, and catechol-O-methyltransferase (COMT) in an MCF10A-derived lineage cell culture model for basal-like human breast cancer progression and in ERα-positive luminal MCF7 breast cancer cells. Low levels of ERα and ERβ mRNA were present in MCF10A-derived cell lines. SULT1E1 mRNA was more abundant in confluent relative to subconfluent MCF10A cells, a non-tumorigenic proliferative breast disease cell line. SULT1E1 was also expressed in preneoplastic MCF10AT1 and MCF10AT1K.cl2 cells, but was markedly repressed in neoplastic MCF10A-derived cell lines as well as in MCF7 cells. Steroid-metabolizing enzymes SULT1A1 and SULT2B1 were only expressed in MCF7 cells. STS and COMT were widely detected across cell lines. Pro-estrogenic 17βHSD1 mRNA was most abundant in neoplastic MCF10CA1a and MCF10DCIS.com cells, while 17βHSD2 mRNA was more prominent in parental MCF10A cells. CYP1B1 mRNA was most abundant in MCF7 cells. Treatment with the histone deacetylase inhibitor trichostatin A (TSA) induced SULT1E1 and CYP19 mRNA but suppressed CYP1B1, STS, COMT, 17βHSD1, and 17βHSD2 mRNA in MCF10A lineage cell lines. In MCF7 cells, TSA treatment suppressed ERα, CYP1B1, STS, COMT, SULT1A1, and SULT2B1 but induced ERβ, CYP19 and SULT2A1 mRNA expression. The results indicate that relative to the MCF7 breast cancer cell line, key determinants of breast estrogen metabolism are differentially regulated in the MCF10A-derived lineage model for breast cancer progression. PMID:19308726

PD-L1 and PD-1 and characterization of tumor-infiltrating lymphocytes in high grade sarcomas of soft tissue - prognostic implications and rationale for immunotherapy.

PubMed

Boxberg, Melanie; Steiger, Katja; Lenze, Ulrich; Rechl, Hans; von Eisenhart-Rothe, Rüdiger; Wörtler, Klaus; Weichert, Wilko; Langer, Rupert; Specht, Katja

2018-01-01

Therapies targeting programmed death 1-(PD-1) or its ligand (PD-L1), promoting antitumor T-cell activity have been successfully introduced into clinical practice. Clinical response correlates with PD-L1 expression by tumor cells or immune cells within the tumor microenvironment. The PD-L1/PD-1 axis and tumor microenvironment has been rarely studied in high-grade sarcomas of soft tissue (hSTS), a group of rare, genetically heterogenous and clinically aggressive tumors. We examined PD-L1 protein and CD274/PD-L1 gene copy number variations in 128 primary resected, therapy-naive hSTS using immunohistochemistry and fluorescence-in-situ hybridization. Frequency of tumoral PD-L1 expression varied widely in different disease subentities, with highest rates of positivity (40%) seen in undifferentiated pleomorphic sarcomas (UPS) and rare positivity detected in synovial sarcomas (6%). Amplification of the CD274/PD-L1 gene occurred in 14% of UPS and was rare in other subtypes. PD-L1 protein expression was significantly more frequent in CD274/PD-L1 amplified cases (p = 0.015). The subgroup of UPS was further characterized regarding the interaction between PD-L1 and the immunologic tumor microenvironment. High density of CD3+ and CD8+ tumor infiltrating lymphocytes (TILs) was significantly correlated with the presence of PD-L1 expression and seen more frequently in tumors with lower TNM stage (p = 0.024). Both, PD-L1 expression and high density lymphocytic infiltration were independent prognostic factors for a favorable overall (p = 0.001, HR 6.105 (2.041-8.258)), disease-specific (p = 0.003, HR 10.536 (2.186-50.774)) and disease-free survival (p = 0.020, HR 3.317 (1.209-9.106); values for CD8) in this particular subgroup of hSTS, whereas PD-L1 expression in TILs or CD274/PD-L1 gene amplification were not associated with outcome. These findings represent novel insights into the immune landscape of soft tissue sarcomas, in particular UPS and strengthen the rationale for immunotherapy, including targeting the PD-1/PD-L1 axis in these tumors.

ISS Flight 2A.2B (STS-106): Commercial Generic Bioprocessing Apparatus (CGBA) Payload BioServe Space Technologies

NASA Technical Reports Server (NTRS)

Stodieck, Louis; Klaus, David

2001-01-01

The two experiments housed in the Commercial Generic Bioprocessing Apparatus (CGBA) during STS-106 were designed to explore how biological processes are affected by microgravity. The first was a developmental study into the effects of microgravity on motor-neuronal growth in the fruit fly species Drosophila melanogaster and the second study was designed to characterize changes in kidney cell gene expression. The objective of the primary experiment, called NIH-B1, was to determine how gravity affects neuronal development of the D. melanogaster embryo and larvae in microgravity, specifically observing the neural connections to muscle fibers.

Genome-wide DNA methylation profiling identifies ALDH1A3 promoter methylation as a prognostic predictor in G-CIMP- primary glioblastoma.

PubMed

Zhang, Wei; Yan, Wei; You, Gan; Bao, Zhaoshi; Wang, Yongzhi; Liu, Yanwei; You, Yongping; Jiang, Tao

2013-01-01

To date, the aberrations in the DNA methylation patterns that are associated with different prognoses of G-CIMP- primary GBMs remain to be elucidated. Here, DNA methylation profiling of primary GBM tissues from 13 long-term survivors (LTS; overall survival ⩾18months) and 20 short-term survivors (STS; overall survival ⩽9months) was performed. Then G-CIMP+ samples were excluded. The differentially expressed CpG loci were identified between residual 18 STS and 9 LTS G-CIMP- samples. Methylation levels of 11 CpG loci (10genes) were statistically significantly lower, and 43 CpG loci (40genes) were statistically significantly higher in the tumor tissues of LTS than those of STS G-CIMP- samples (P<0.01). Of the 43 CpG loci that were hypermethylated in LTS G-CIMP- samples, 3 CpG loci localized in the promoter of ALDH1A3. Furthermore, using an independent validation cohort containing 37 primary GBM samples without IDH1 mutation and MGMT promoter methylation, the hypermethylation status of ALDH1A3 promoter predicted a better prognosis with an accompanied low expression of ALDH1A3 protein. Taken together, our results defined prognosis-related methylation signatures systematically for the first time in G-CIMP- primary GBMs. ALDH1A3 promoter methylation conferred a favorable prognosis in G-CIMP- primary GBMs. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Arabidopsis gene expression patterns are altered during spaceflight

NASA Astrophysics Data System (ADS)

Paul, Anna-Lisa; Popp, Michael P.; Gurley, William B.; Guy, Charles; Norwood, Kelly L.; Ferl, Robert J.

The exposure of Arabidopsis thaliana (Arabidopsis) plants to spaceflight environments results in differential gene expression. A 5-day mission on orbiter Columbia in 1999 (STS-93) carried transgenic Arabidopsis plants engineered with a transgene composed of the alcohol dehydrogenase (Adh) gene promoter linked to the β-Glucuronidase (GUS) reporter gene. The plants were used to evaluate the effects of spaceflight on gene expression patterns initially by using the Adh/GUS transgene to address specifically the possibility that spaceflight induces a hypoxic stress response (Paul, A.L., Daugherty, C.J., Bihn, E.A., Chapman, D.K., Norwood, K.L., Ferl, R.J., 2001. Transgene expression patterns indicate that spaceflight affects stress signal perception and transduction in arabidopsis, Plant Physiol. 126, 613-621). As a follow-on to the reporter gene analysis, we report here the evaluation of genome-wide patterns of native gene expression within Arabidopsis shoots utilizing the Agilent DNA array of 21,000 Arabidopsis genes. As a control for the veracity of the array analyses, a selection of genes was further characterized with quantitative Real-Time RT PCR (ABI - Taqman®). Comparison of the patterns of expression for arrays probed with RNA isolated from plants exposed to spaceflight compared to RNA isolated from ground control plants revealed 182 genes that were differentially expressed in response to the spaceflight mission by more than 4-fold, and of those only 50 genes were expressed at levels chosen to support a conservative change call. None of the genes that are hallmarks of hypoxic stress were induced to this level. However, genes related to heat shock were dramatically induced - but in a pattern and under growth conditions that are not easily explained by elevated temperatures. These gene expression data are discussed in light of current models for plant responses to the spaceflight environment and with regard to potential future spaceflight experiment opportunities.

Sesquiterpene Synthase-3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase Fusion Protein Responsible for Hirsutene Biosynthesis in Stereum hirsutum.

PubMed

Flynn, Christopher M; Schmidt-Dannert, Claudia

2018-06-01

The wood-rotting mushroom Stereum hirsutum is a known producer of a large number of namesake hirsutenoids, many with important bioactivities. Hirsutenoids form a structurally diverse and distinct class of sesquiterpenoids. No genes involved in hirsutenoid biosynthesis have yet been identified or their enzymes characterized. Here, we describe the cloning and functional characterization of a hirsutene synthase as an unexpected fusion protein of a sesquiterpene synthase (STS) with a C-terminal 3-hydroxy-3-methylglutaryl-coenzyme A (3-hydroxy-3-methylglutaryl-CoA) synthase (HMGS) domain. Both the full-length fusion protein and truncated STS domain are highly product-specific 1,11-cyclizing STS enzymes with kinetic properties typical of STSs. Complementation studies in Saccharomyces cerevisiae confirmed that the HMGS domain is also functional in vivo Phylogenetic analysis shows that the hirsutene synthase domain does not form a clade with other previously characterized sesquiterpene synthases from Basidiomycota. Comparative gene structure analysis of this hirsutene synthase with characterized fungal enzymes reveals a significantly higher intron density, suggesting that this enzyme may be acquired by horizontal gene transfer. In contrast, the HMGS domain is clearly related to other fungal homologs. This STS-HMGS fusion protein is part of a biosynthetic gene cluster that includes P450s and oxidases that are expressed and could be cloned from cDNA. Finally, this unusual fusion of a terpene synthase to an HMGS domain, which is not generally recognized as a key regulatory enzyme of the mevalonate isoprenoid precursor pathway, led to the identification of additional HMGS duplications in many fungal genomes, including the localization of HMGSs in other predicted sesquiterpenoid biosynthetic gene clusters. IMPORTANCE Hirsutenoids represent a structurally diverse class of bioactive sesquiterpenoids isolated from fungi. Identification of their biosynthetic pathways will provide access to this chemodiversity for the discovery and synthesis of molecules with new bioactivities. The identification and successful cloning of the previously elusive hirsutene synthase from the S. hirsutum provide important insights and strategies for biosynthetic gene discovery in Basidiomycota. The finding of a terpene synthase-HMGS fusion, the discovery of other sesquiterpenoid biosynthetic gene clusters with dedicated HMGS genes, and HMGS gene duplications in fungal genomes give new importance to the role of HMGS as a key regulatory enzyme in isoprenoid and sterol biosynthesis that should be exploited for metabolic engineering. Copyright © 2018 American Society for Microbiology.

Alteration of gene expression profiles in skeletal muscle of rats exposed to microgravity during a spaceflight

NASA Technical Reports Server (NTRS)

Taylor, Wayne E.; Bhasin, Shalender; Lalani, Rukhsana; Datta, Anuj; Gonzalez-Cadavid, Nestor F.

2002-01-01

To clarify the mechanism of skeletal muscle wasting during spaceflights, we investigated whether intramuscular gene expression profiles are affected, by using DNA microarray methods. Male rats sent on the 17-day NASA STS-90 Neurolab spaceflight were sacrificed 24 hours after return to earth (MG group). Ground control rats were maintained for 17 days in flight-simulated cages (CS group). Spaceflight induced a 19% and 23% loss of tibialis anterior and gastrocnemius muscle mass, respectively, as compared to ground controls. Muscle RNA was analyzed by the Clontech Atlas DNA expression array in four rats, with two MG/ CS pairs for the tibialis anterior, and one pair for the gastrocnemius. Alterations in gene expression were verified for selected genes by reverse-transcription PCR. In both muscles of MG rats, mRNAs for 12 genes were up-regulated by over 2-fold, and 38 were down-regulated compared to controls. There was inhibition of genes for cell proliferation and growth factor cascades, including cell cycle genes and signal transduction proteins, such as p21 Cip1, retinoblastoma (Rb), cyclins G1/S, -E and -D3, MAP kinase 3, MAD3, and ras related protein RAB2. These data indicate that following exposure to microgravity, there is downregulation of genes involved in regulation of muscle satellite cell replication.

Enhanced Tolerance of Transgenic Potato Plants Over-Expressing Non-specific Lipid Transfer Protein-1 (StnsLTP1) against Multiple Abiotic Stresses

PubMed Central

Gangadhar, Baniekal H.; Sajeesh, Kappachery; Venkatesh, Jelli; Baskar, Venkidasamy; Abhinandan, Kumar; Yu, Jae W.; Prasad, Ram; Mishra, Raghvendra K.

2016-01-01

Abiotic stresses such as heat, drought, and salinity are major environmental constraints that limit potato (Solanum tuberosum L.) production worldwide. Previously, we found a potential thermo-tolerance gene, named StnsLTP1 from potato using yeast functional screening. Here, we report the functional characterization of StnsLTP1 and its role in multiple abiotic stresses in potato plants. Computational analysis of StnsLTP1 with other plant LTPs showed eight conserved cysteine residues, and four α-helices stabilized by four disulfide bridges. Expression analysis of StnsLTP1 gene showed differential expression under heat, water-deficit and salt stresses. Transgenic potato lines over-expressing StnsLTP1 gene displayed enhanced cell membrane integrity under stress conditions, as indicated by reduced membrane lipid per-oxidation, and hydrogen peroxide content relative to untransformed (UT) control plants. In addition, transgenic lines over-expressing StLTP1 also exhibited increased antioxidant enzyme activity with enhanced accumulation of ascorbates, and up-regulation of stress-related genes including StAPX, StCAT, StSOD, StHsfA3, StHSP70, and StsHSP20 compared with the UT plants. These results suggests that StnsLTP1 transgenic plants acquired improved tolerance to multiple abiotic stresses through enhanced activation of antioxidative defense mechanisms via cyclic scavenging of reactive oxygen species and regulated expression of stress-related genes. PMID:27597854

Biological and metabolic response in STS-135 space-flown mouse skin.

PubMed

Mao, X W; Pecaut, M J; Stodieck, L S; Ferguson, V L; Bateman, T A; Bouxsein, M L; Gridley, D S

2014-08-01

There is evidence that space flight condition-induced biological damage is associated with increased oxidative stress and extracellular matrix (ECM) remodeling. To explore possible mechanisms, changes in gene expression profiles implicated in oxidative stress and in ECM remodeling in mouse skin were examined after space flight. The metabolic effects of space flight in skin tissues were also characterized. Space Shuttle Atlantis (STS-135) was launched at the Kennedy Space Center on a 13-day mission. Female C57BL/6 mice were flown in the STS-135 using animal enclosure modules (AEMs). Within 3-5 h after landing, the mice were euthanized and skin samples were harvested for gene array analysis and metabolic biochemical assays. Many genes responsible for regulating production and metabolism of reactive oxygen species (ROS) were significantly (p < 0.05) altered in the flight group, with fold changes >1.5 compared to AEM control. For ECM profile, several genes encoding matrix and metalloproteinases involved in ECM remodeling were significantly up-/down-regulated following space flight. To characterize the metabolic effects of space flight, global biochemical profiles were evaluated. Of 332 named biochemicals, 19 differed significantly (p < 0.05) between space flight skin samples and AEM ground controls, with 12 up-regulated and 7 down-regulated including altered amino acid, carbohydrate metabolism, cell signaling, and transmethylation pathways. Collectively, the data demonstrated that space flight condition leads to a shift in biological and metabolic homeostasis as the consequence of increased regulation in cellular antioxidants, ROS production, and tissue remodeling. This indicates that astronauts may be at increased risk for pathophysiologic damage or carcinogenesis in cutaneous tissue.

O-acetyltransferase gene neuO is segregated according to phylogenetic background and contributes to environmental desiccation resistance in Escherichia coli K1.

PubMed

Mordhorst, Ines L; Claus, Heike; Ewers, Christa; Lappann, Martin; Schoen, Christoph; Elias, Johannes; Batzilla, Julia; Dobrindt, Ulrich; Wieler, Lothar H; Bergfeld, Anne K; Mühlenhoff, Martina; Vogel, Ulrich

2009-12-01

Escherichia coli K1 causes disease in humans and birds. Its polysialic acid capsule can be O-acetylated via phase-variable expression of the acetyltransferase NeuO encoded by prophage CUS-3. The role of capsule O-acetylation in ecological adaptation or pathogenic invasion of E. coli K1 is largely unclear. A population genetics approach was performed to study the distribution of neuO among E. coli K1 isolates from human and avian sources. Multilocus sequence typing revealed 39 different sequence types (STs) among 183 E. coli K1 strains. The proportion of the ST95 complex (STC95) was 44%. NeuO was found in 98% of the STC95 strains, but only in 24% of other STs. Grouping of STs and prophage genotypes revealed a segregation of prophage types according to STs, suggesting coevolution of CUS-3 and the E. coli K1 host. Within the STC95, which is known to harbour both human and avian pathogenic isolates, CUS-3 genotypes were shared irrespective of the host species. Functional analysis of a variety of strain pairs revealed that NeuO-mediated K1 capsule O-acetylation enhanced desiccation resistance. In contrast, NeuO expression led to a reduced biofilm formation in biofilm positive E. coli K1 isolates. These findings suggest a delicate ecological balance of neuO'on'/'off' switching.

Alterations in TNF- and IL-related gene expression in space-flown WI38 human fibroblasts

NASA Technical Reports Server (NTRS)

Semov, Alexandre; Semova, Nathalia; Lacelle, Chantale; Marcotte, Richard; Petroulakis, Emmanuel; Proestou, Gregory; Wang, Eugenia

2002-01-01

Spaceflight, just like aging, causes profound changes in musculoskeletal parameters, which result in decreased bone density and muscular weakness. As these conditions decrease our ability to conduct long-term manned space missions, and increase bone frailty in the elderly, the identification of genes responsible for the apparition of these physiological changes will be of great benefit. Thus, we developed and implemented a new microarray approach to investigate the changes in normal WI38 human fibroblast gene expression that arise as a consequence of space flight. Using our microarray, we identified changes in the level of expression of 10 genes, belonging to either the tumor necrosis factor- (TNF) or interleukin- (IL) related gene families in fibroblasts when WI38 cells exposed to microgravity during the STS-93 Space Shuttle mission were compared with ground controls. The genes included two ligands from the TNF superfamily, TWEAK and TNFSF15; two TNF receptor-associated proteins, NSMAF and PTPN13; three TNF-inducible genes, ABC50, PTX3, and SCYA13; TNF-alpha converting enzyme, IL-1 receptor antagonist, and IL-15 receptor alpha chain. Most of these are involved in either the regulation of bone density, and as such the development of spaceflight osteopenia, or in the development of proinflammatory status.

[Genetic analysis of two cases with Dandy-Walker deformed fetus].

PubMed

Yao, Juan; Fang, Rong; Shen, Xueping; Shen, Guosong; Zhang, Su

2017-10-10

To explore the genetic etiology of two fetuses with Dandy-Walker malformation using single nucleotide polymorphism microarray (SNP-array). The fetuses and their parents were subjected to G banding karyotype analysis. The fetuses were also subjected to SNP-array analysis. The parents of both fetuses showed a normal karyotype. One fetus has a 46,X,?i(X)(q10), while for another conventional cell culture has failed. SNP-array showed that one fetus carried a 6p25.3p25.2 microdeletion, and another carried a Xp22.33p22.2 deletion and a Yq11.221q11 duplication. The abnormal fragments have involved FOXC1, SHOX and STS genes, which are associated with Dandy-Walker malformation. Alteration of 6p25.3p25.2, Xp22.33p22.2 copy numbers probably underlies the Dandy-Walker syndrome in the fetuses. The disorder may be attributed to abnormal expression of FOXC1, SHOX, and STS genes. SNP-array can provide an important supplement for prenatal diagnosis.

The Maize PI/GLO Ortholog Zmm16/sterile tassel silky ear1 Interacts with the Zygomorphy and Sex Determination Pathways in Flower Development[OPEN

PubMed Central

Bartlett, Madelaine E.; Williams, Steven K.; Taylor, Zac; DeBlasio, Stacy; Hall, Darren H.; Schmidt, Robert J.; Jackson, David P.

2015-01-01

In monocots and eudicots, B class function specifies second and third whorl floral organ identity as described in the classic ABCE model. Grass B class APETALA3/DEFICIENS orthologs have been functionally characterized; here, we describe the positional cloning and characterization of a maize (Zea mays) PISTILLATA/GLOBOSA ortholog Zea mays mads16 (Zmm16)/sterile tassel silky ear1 (sts1). We show that, similar to many eudicots, all the maize B class proteins bind DNA as obligate heterodimers and positively regulate their own expression. However, sts1 mutants have novel phenotypes that provide insight into two derived aspects of maize flower development: carpel abortion and floral asymmetry. Specifically, we show that carpel abortion acts downstream of organ identity and requires the growth-promoting factor grassy tillers1 and that the maize B class genes are expressed asymmetrically, likely in response to zygomorphy of grass floral primordia. Further investigation reveals that floral phyllotactic patterning is also zygomorphic, suggesting significant mechanistic differences with the well-characterized models of floral polarity. These unexpected results show that despite extensive study of B class gene functions in diverse flowering plants, novel insights can be gained from careful investigation of homeotic mutants outside the core eudicot model species. PMID:26518212

Verification of STS markers for leaf rust resistance genes of wheat by seven European laboratories.

PubMed

Błaszczyk, Lidia; Chełkowski, Jerzy; Korzun, Victor; Kraic, Jan; Ordon, Frank; Ovesná, Jaroslava; Purnhauser, Laszlo; Tar, Melinda; Vida, Gyula

2004-01-01

A set of Thatcher near-isogenic lines and two breeding lines were used to examine sequence tagged site (STS) markers linked to leaf rust resistance genes Lr9, Lr10, Lr19, Lr24, Lr28, Lr29, Lr35, and a simple sequenced repeat (SSR) marker for Lr39. The selected STS markers for resistance genes Lr9, Lr10, Lr19, Lr24 and Lr28 were identified in seven accessions by seven European laboratories. Near-isogenic lines of the spring wheat Thatcher were used as positive controls. Markers for resistance genes Lr9, Lr10, Lr19, Lr24 were identified in all seven laboratories as amplification products of 1100 bp, 310 bp, 130 bp and 310 bp, respectively. The STS markers linked to resistance genes Lr9, Lr10, Lr19, Lr24, Lr29, Lr35 and the SSR marker for Lr39 were robust and highly specific for these genes and will be useful in marker-assisted selection in wheat. However, the amplification product of 378 bp that corresponded with resistance gene Lr28 was detected in all accessions including genotypes lacking this gene in all seven laboratories. This marker needs to be improved.

Stilbene content and expression of stilbene synthase genes in cell cultures of Vitis amurensis treated with cinnamic and caffeic acids.

PubMed

Tyunin, Alexey P; Nityagovsky, Nikolay N; Grigorchuk, Valeria P; Kiselev, Konstantin V

2018-03-01

It has previously been shown that exogenous application of p-coumaric acid (CA), a precursor of phenolic compounds, improved stilbene production in cell cultures of Vitis amurensis. This study examines the effect of cinnamic (Cin) and caffeic (Caf) acids, which are also phenolic precursors, on stilbene biosynthesis in the cell cultures. Five stilbenes, t-resveratrol diglucoside, t-piceid (t-resveratrol glucoside), t-resveratrol, t-ε-viniferin, and t-δ-viniferin, were found in the treated and untreated cells. Cin acid increased the total stilbene production in the grape cell cultures 2.3-3.5 times in comparison with that in the untreated cells. Caf acid increased the total stilbene production by 1.8- to 1.9-fold, but this increase was not considerably different from stilbene production in the untreated cells. Cin acid affected the total stilbene production via a marked increase in the content of t-resveratrol diglucoside (up to 2.2 times), t-piceid (up to three times), t-resveratrol (up to 5.1 times), t-ε-viniferin (up to eight times), and t-δ-viniferin (up to 9.2 times). Transcription levels of VaSTS5, 6, 7, 8, and 10 genes considerably increased under 0.1, 0.25, and 0.5 mM Cin acid. These results indicate that Cin acid increased stilbene production in V. amurensis calli via a selective enhancement of STS gene expression. © 2017 International Union of Biochemistry and Molecular Biology, Inc.

Cloning of the anhidrotic ectodermal dysplasia gene: Identification of cDNAs associated with CpG islands mapped near translocation breakpoint in two female patients

DOE Office of Scientific and Technical Information (OSTI.GOV)

Srivastava, A.K.; Schlessinger, D.; Kere, J.

1994-09-01

The gene for the X chromosomal developmental disorder anhidrotic ectodermal dysplasia (EDA) has been mapped to Xq12-q13 by linkage analysis and is expressed in a few females with chromosomal translocations involving band Xq12-q13. A yeast artificial chromosome (YAC) contig (2.0 Mb) spanning two translocation breakpoints has been assembled by sequence-tagged site (STS)-based chromosomal walking. The two translocation breakpoints (X:autosome translocations from the affected female patients) have been mapped less than 60 kb apart within a YAC contig. Unique probes and intragenic STSs (mapped between the two translocations) have been developed and a somatic cell hybrid carrying the translocated X chromosomemore » from the AK patient has been analyzed by isolating unique probes that span the breakpoint. Several STSs made from intragenic sequences have been found to be conserved in mouse, hamster and monkey, but we have detected no mRNAs in a number of tissues tested. However, a probe and STS developed from the DNA spanning the AK breakpoint is conserved in mouse, hamster and monkey, and we have detected expressed sequences in skin cells and cDNA libraries. In addition, unique sequences have been obtained from two CpG islands in the region that maps proximal to the breakpoints. cDNAs containing these sequences are being studied as candidates for the gene affected in the etiology of EDA.« less

Assembly of YAC contigs on the long arm of human chromosome 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, J.; Fujiwara, T.M.; Wang, J.X.

1994-09-01

We have previously identified approximately 2,000 chromosome 2-specific YACs by screening the CEPH Mark I YAC library (`Midi- YACs`). Using STS content mapping, we have been able to order groups of these YACs along chromosome 2q. The four biggest YAC groups were associated with VIL (2q35), FN (2q34), PAX3 (2q36), ALPI (2q37) and contained 113, 107, 79, and 63 YACs, respectively. We have identified the minimal tiling paths for most YAC groups and determined the insert sizes of over 300 YACs. Furthermore, on human chromosome 2q31-q37, 15 microsatellite markers were linked to various expressed genes through overlapping YACs and themore » physical distance of microsatellites to expressed genes was determined. The precise mapping of a set of highly informative microsatellite markers with respect to known genes provides a useful tool for linkage studies and the identification of disease genes from the long arm of human chromosome 2.« less

Responses in the right posterior superior temporal sulcus show a feature-based response to facial expression.

PubMed

Flack, Tessa R; Andrews, Timothy J; Hymers, Mark; Al-Mosaiwi, Mohammed; Marsden, Samuel P; Strachan, James W A; Trakulpipat, Chayanit; Wang, Liang; Wu, Tian; Young, Andrew W

2015-08-01

The face-selective region of the right posterior superior temporal sulcus (pSTS) plays an important role in analysing facial expressions. However, it is less clear how facial expressions are represented in this region. In this study, we used the face composite effect to explore whether the pSTS contains a holistic or feature-based representation of facial expression. Aligned and misaligned composite images were created from the top and bottom halves of faces posing different expressions. In Experiment 1, participants performed a behavioural matching task in which they judged whether the top half of two images was the same or different. The ability to discriminate the top half of the face was affected by changes in the bottom half of the face when the images were aligned, but not when they were misaligned. This shows a holistic behavioural response to expression. In Experiment 2, we used fMR-adaptation to ask whether the pSTS has a corresponding holistic neural representation of expression. Aligned or misaligned images were presented in blocks that involved repeating the same image or in which the top or bottom half of the images changed. Increased neural responses were found in the right pSTS regardless of whether the change occurred in the top or bottom of the image, showing that changes in expression were detected across all parts of the face. However, in contrast to the behavioural data, the pattern did not differ between aligned and misaligned stimuli. This suggests that the pSTS does not encode facial expressions holistically. In contrast to the pSTS, a holistic pattern of response to facial expression was found in the right inferior frontal gyrus (IFG). Together, these results suggest that pSTS reflects an early stage in the processing of facial expression in which facial features are represented independently. Copyright © 2015 Elsevier Ltd. All rights reserved.

Thirty days of spaceflight does not alter murine calvariae structure despite increased Sost expression.

PubMed

Macaulay, Timothy R; Siamwala, Jamila H; Hargens, Alan R; Macias, Brandon R

2017-12-01

Previously our laboratory documented increases in calvaria bone volume and thickness in mice exposed to 15 days of spaceflight aboard the NASA Shuttle mission STS-131. However, the tissues were not processed for gene expression studies to determine what bone formation pathways might contribute to these structural adaptations. Therefore, this study was designed to investigate both the structural and molecular changes in mice calvariae after a longer duration of spaceflight. The primary purpose was to determine the calvaria bone volume and thickness of mice exposed to 30 days of spaceflight using micro-computed tomography for comparison with our previous findings. Because sclerostin, the secreted glycoprotein of the Sost gene, is a potent inhibitor of bone formation, our second aim was to quantify Sost mRNA expression using quantitative PCR. Calvariae were obtained from six mice aboard the Russian 30-day Bion-M1 biosatellite and seven ground controls. In mice exposed to 30 days of spaceflight, calvaria bone structure was not significantly different from that of their controls (bone volume was about 5% lower in spaceflight mice, p = 0.534). However, Sost mRNA expression was 16-fold (16.4 ± 0.4, p < 0.001) greater in the spaceflight group than that in the ground control group. Therefore, bone formation may have been suppressed in mice exposed to 30 days of spaceflight. Genetic responsiveness (e.g. sex or strain of animals) or in-flight environmental conditions other than microgravity (e.g. pCO 2 levels) may have elicited different bone adaptations in STS-131 and Bion-M1 mice. Although structural results were not significant, this study provides biochemical evidence that calvaria mechanotransduction pathways may be altered during spaceflight, which could reflect vascular and interstitial fluid adaptations in non-weight bearing bones. Future studies are warranted to elucidate the processes that mediate these effects and the factors responsible for discordant calvaria bone adaptations between STS-131 and Bion-M1 mice.

Fine mapping of the NRC-1 tumor suppressor locus within chromosome 3p12.

PubMed

Zhang, Kun; Lott, Steven T; Jin, Li; Killary, Ann McNeill

2007-08-31

Identification of tumor suppressor genes based on physical mapping exercises has proven to be a challenging endeavor, due to the difficulty of narrowing regions of loss of heterozygosity (LOH), infrequency of homozygous deletions, and the labor-intensive characterization process for screening candidates in a given genomic interval. We previously defined a chromosome 3p12 tumor suppressor locus NRC-1 (Nonpapillary Renal Carcinoma-1) by functional complementation experiments in which renal cell carcinoma microcell hybrids containing introduced normal chromosome 3p fragments were either suppressed or unsuppressed for tumorigenicity following injection into athymic nude mice. We now present the fine-scale physical mapping of NRC-1 using a QPCR-based approach for measuring copy number at sequence tagged sites (STS) which allowed a sub-exon mapping resolution. Using STS-QPCR and a novel statistical algorithm, the NRC-1 locus was narrowed to 4.615-Mb with the distal boundary mapping within a 38-Kb interval between exon 3 and exon 4 of the DUTT1/Robo1 gene, currently the only candidate tumor suppressor gene in the interval. Further mutational screening and gene expression analyses indicate that DUTT1/ROBO1 is not involved in the tumor suppressor activity of NRC-1, suggesting that there are at least two important tumor suppressor genes within the chromosome 3p12 interval.

Which missing value imputation method to use in expression profiles: a comparative study and two selection schemes.

PubMed

Brock, Guy N; Shaffer, John R; Blakesley, Richard E; Lotz, Meredith J; Tseng, George C

2008-01-10

Gene expression data frequently contain missing values, however, most down-stream analyses for microarray experiments require complete data. In the literature many methods have been proposed to estimate missing values via information of the correlation patterns within the gene expression matrix. Each method has its own advantages, but the specific conditions for which each method is preferred remains largely unclear. In this report we describe an extensive evaluation of eight current imputation methods on multiple types of microarray experiments, including time series, multiple exposures, and multiple exposures x time series data. We then introduce two complementary selection schemes for determining the most appropriate imputation method for any given data set. We found that the optimal imputation algorithms (LSA, LLS, and BPCA) are all highly competitive with each other, and that no method is uniformly superior in all the data sets we examined. The success of each method can also depend on the underlying "complexity" of the expression data, where we take complexity to indicate the difficulty in mapping the gene expression matrix to a lower-dimensional subspace. We developed an entropy measure to quantify the complexity of expression matrixes and found that, by incorporating this information, the entropy-based selection (EBS) scheme is useful for selecting an appropriate imputation algorithm. We further propose a simulation-based self-training selection (STS) scheme. This technique has been used previously for microarray data imputation, but for different purposes. The scheme selects the optimal or near-optimal method with high accuracy but at an increased computational cost. Our findings provide insight into the problem of which imputation method is optimal for a given data set. Three top-performing methods (LSA, LLS and BPCA) are competitive with each other. Global-based imputation methods (PLS, SVD, BPCA) performed better on mcroarray data with lower complexity, while neighbour-based methods (KNN, OLS, LSA, LLS) performed better in data with higher complexity. We also found that the EBS and STS schemes serve as complementary and effective tools for selecting the optimal imputation algorithm.

Dissimilar processing of emotional facial expressions in human and monkey temporal cortex

PubMed Central

Zhu, Qi; Nelissen, Koen; Van den Stock, Jan; De Winter, François-Laurent; Pauwels, Karl; de Gelder, Beatrice; Vanduffel, Wim; Vandenbulcke, Mathieu

2013-01-01

Emotional facial expressions play an important role in social communication across primates. Despite major progress made in our understanding of categorical information processing such as for objects and faces, little is known, however, about how the primate brain evolved to process emotional cues. In this study, we used functional magnetic resonance imaging (fMRI) to compare the processing of emotional facial expressions between monkeys and humans. We used a 2 × 2 × 2 factorial design with species (human and monkey), expression (fear and chewing) and configuration (intact versus scrambled) as factors. At the whole brain level, selective neural responses to conspecific emotional expressions were anatomically confined to the superior temporal sulcus (STS) in humans. Within the human STS, we found functional subdivisions with a face-selective right posterior STS area that also responded selectively to emotional expressions of other species and a more anterior area in the right middle STS that responded specifically to human emotions. Hence, we argue that the latter region does not show a mere emotion-dependent modulation of activity but is primarily driven by human emotional facial expressions. Conversely, in monkeys, emotional responses appeared in earlier visual cortex and outside face-selective regions in inferior temporal cortex that responded also to multiple visual categories. Within monkey IT, we also found areas that were more responsive to conspecific than to non-conspecific emotional expressions but these responses were not as specific as in human middle STS. Overall, our results indicate that human STS may have developed unique properties to deal with social cues such as emotional expressions. PMID:23142071

Transgene expression patterns indicate that spaceflight affects stress signal perception and transduction in arabidopsis.

PubMed

Paul, A L; Daugherty, C J; Bihn, E A; Chapman, D K; Norwood, K L; Ferl, R J

2001-06-01

The use of plants as integral components of life support systems remains a cornerstone of strategies for long-term human habitation of space and extraterrestrial colonization. Spaceflight experiments over the past few decades have refined the hardware required to grow plants in low-earth orbit and have illuminated fundamental issues regarding spaceflight effects on plant growth and development. Potential incipient hypoxia, resulting from the lack of convection-driven gas movement, has emerged as a possible major impact of microgravity. We developed transgenic Arabidopsis containing the alcohol dehydrogenase (Adh) gene promoter linked to the beta-glucuronidase (GUS) reporter gene to address specifically the possibility that spaceflight induces the plant hypoxia response and to assess whether any spaceflight response was similar to control terrestrial hypoxia-induced gene expression patterns. The staining patterns resulting from a 5-d mission on the orbiter Columbia during mission STS-93 indicate that the Adh/GUS reporter gene was activated in roots during the flight. However, the patterns of expression were not identical to terrestrial control inductions. Moreover, although terrestrial hypoxia induces Adh/GUS expression in the shoot apex, no apex staining was observed in the spaceflight plants. This indicates that either the normal hypoxia response signaling is impaired in spaceflight or that spaceflight inappropriately induces Adh/GUS activity for reasons other than hypoxia.

Transgene expression patterns indicate that spaceflight affects stress signal perception and transduction in arabidopsis

NASA Technical Reports Server (NTRS)

Paul, A. L.; Daugherty, C. J.; Bihn, E. A.; Chapman, D. K.; Norwood, K. L.; Ferl, R. J.

2001-01-01

The use of plants as integral components of life support systems remains a cornerstone of strategies for long-term human habitation of space and extraterrestrial colonization. Spaceflight experiments over the past few decades have refined the hardware required to grow plants in low-earth orbit and have illuminated fundamental issues regarding spaceflight effects on plant growth and development. Potential incipient hypoxia, resulting from the lack of convection-driven gas movement, has emerged as a possible major impact of microgravity. We developed transgenic Arabidopsis containing the alcohol dehydrogenase (Adh) gene promoter linked to the beta-glucuronidase (GUS) reporter gene to address specifically the possibility that spaceflight induces the plant hypoxia response and to assess whether any spaceflight response was similar to control terrestrial hypoxia-induced gene expression patterns. The staining patterns resulting from a 5-d mission on the orbiter Columbia during mission STS-93 indicate that the Adh/GUS reporter gene was activated in roots during the flight. However, the patterns of expression were not identical to terrestrial control inductions. Moreover, although terrestrial hypoxia induces Adh/GUS expression in the shoot apex, no apex staining was observed in the spaceflight plants. This indicates that either the normal hypoxia response signaling is impaired in spaceflight or that spaceflight inappropriately induces Adh/GUS activity for reasons other than hypoxia.

Genetic Regulation of Guanylate-Binding Proteins 2b and 5 during Leishmaniasis in Mice

PubMed Central

Sohrabi, Yahya; Volkova, Valeryia; Kobets, Tatyana; Havelková, Helena; Krayem, Imtissal; Slapničková, Martina; Demant, Peter; Lipoldová, Marie

2018-01-01

Interferon-induced GTPases [guanylate-binding proteins (GBPs)] play an important role in inflammasome activation and mediate innate resistance to many intracellular pathogens, but little is known about their role in leishmaniasis. We therefore studied expression of Gbp2b/Gbp1 and Gbp5 mRNA in skin, inguinal lymph nodes, spleen, and liver after Leishmania major infection and in uninfected controls. We used two different groups of related mouse strains: BALB/c, STS, and CcS-5, CcS-16, and CcS-20 that carry different combinations of BALB/c and STS genomes, and strains O20, C57BL/10 (B10) and B10.O20, OcB-9, and OcB-43 carrying different combinations of O20 and B10 genomes. The strains were classified on the basis of size and number of infection-induced skin lesions as highly susceptible (BALB/c, CcS-16), susceptible (B10.O20), intermediate (CcS-20), and resistant (STS, O20, B10, OcB-9, OcB-43). Some uninfected strains differed in expression of Gbp2b/Gbp1 and Gbp5, especially of Gbp2b/Gbp1 in skin. Uninfected BALB/c and STS did not differ in their expression, but in CcS-5, CcS-16, and CcS-20, which all carry BALB/c-derived Gbp gene-cluster, expression of Gbp2b/Gbp1 exceeds that of both parents. These data indicate trans-regulation of Gbps. Infection resulted in approximately 10× upregulation of Gbp2b/Gbp1 and Gbp5 mRNAs in organs of both susceptible and resistant strains, which was most pronounced in skin. CcS-20 expressed higher level of Gbp2b/Gbp1 than both parental strains in skin, whereas CcS-16 expressed higher level of Gbp2b/Gbp1 than both parental strains in skin and liver. This indicates a trans-regulation present in infected mice CcS-16 and CcS-20. Immunostaining of skin of five strains revealed in resistant and intermediate strains STS, CcS-5, O20, and CcS-20 tight co-localization of Gbp2b/Gbp1 protein with most L. major parasites, whereas in the highly susceptible strain, BALB/c most parasites did not associate with Gbp2b/Gbp1. In conclusion, expression of Gbp2b/Gbp1 and Gbp5 was increased even in organs of clinically asymptomatic resistant mice. It suggests a hidden inflammation, which might contribute to control of persisting parasites. This is supported by the co-localization of Gbpb2/Gbp1 protein and L. major parasites in skin of resistant and intermediate but not highly susceptible mice. PMID:29467757

Possible role of the aromatase-independent steroid metabolism pathways in hormone responsive primary breast cancers.

PubMed

Hanamura, Toru; Niwa, Toshifumi; Gohno, Tatsuyuki; Kurosumi, Masafumi; Takei, Hiroyuki; Yamaguchi, Yuri; Ito, Ken-ichi; Hayashi, Shin-ichi

2014-01-01

Aromatase inhibitors (AIs) exert antiproliferative effects by reducing local estrogen production from androgens in postmenopausal women with hormone-responsive breast cancer. Previous reports have shown that androgen metabolites generated by the aromatase-independent enzymes, 5α-androstane-3β, 17β-diol (3β-diol), androst-5-ene-3β, and 17β-diol (A-diol), also activate estrogen receptor (ER) α. Estradiol (E2) can also reportedly be generated from estrone sulfate (E1S) pooled in the plasma. Estrogenic steroid-producing aromatase-independent pathways have thus been proposed as a mechanism of AI resistance. However, it is unclear whether these pathways are functional in clinical breast cancer. To investigate this issue, we assessed the transcriptional activities of ER in 45 ER-positive human breast cancers using the adenovirus estrogen-response element-green fluorescent protein assay and mRNA expression levels of the ER target gene, progesterone receptor, as indicators of ex vivo and in vivo ER activity, respectively. We also determined mRNA expression levels of 5α-reductase type 1 (SRD5A1) and 3β-hydroxysteroid dehydrogenase type 1 (3β-HSD type 1; HSD3B1), which produce 3β-diol from androgens, and of steroid sulfatase (STS) and 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD type 1; HSD17B1), which produce E2 or A-diol from E1S or dehydroepiandrosterone sulfate. SRD5A1 and HSD3B1 expression levels were positively correlated with ex vivo and in vivo ER activities. STS and HSD17B1 expression levels were positively correlated with in vivo ER activity alone. Elevated expression levels of these steroid-metabolizing enzymes in association with high in vivo ER activity were particularly notable in postmenopausal patients. Analysis of the expression levels of steroid-metabolizing enzymes revealed positive correlations between SRD5A1 and HSD3B1, and STS and HSD17B1. These findings suggest that the SRD5A1-HSD3B1 as well as the STS-HSD17B pathways, could contributes to ER activation, especially postmenopause. These pathways might function as an alternative estrogenic steroid-producing, aromatase-independent pathways.

Radiogenomic analysis of hypoxia pathway reveals computerized MRI descriptors predictive of overall survival in glioblastoma

NASA Astrophysics Data System (ADS)

Beig, Niha; Patel, Jay; Prasanna, Prateek; Partovi, Sasan; Varadan, Vinay; Madabhushi, Anant; Tiwari, Pallavi

2017-03-01

Glioblastoma Multiforme (GBM) is a highly aggressive brain tumor with a median survival of 14 months. Hypoxia is a hallmark trait in GBM that is known to be associated with angiogenesis, tumor growth, and resistance to conventional therapy, thereby limiting treatment options for GBM patients. There is thus an urgent clinical need for non-invasively capturing tumor hypoxia in GBM towards identifying a subset of patients who would likely benefit from anti-angiogenic therapies (bevacizumab) in the adjuvant setting. In this study, we employed radiomic descriptors to (a) capture molecular variations of tumor hypoxia on routine MRI that are otherwise not appreciable; and (b) employ the radiomic correlates of hypoxia to discriminate patients with short-term survival (STS, overall survival (OS) < 7 months), mid-term survival (MTS) (7 months16 months). A total of 97 studies (25 STS, 36 MTS, 36 LTS) with Gadolinium T1-contrast (Gd-T1c), T2w, and FLAIR protocols with their corresponding gene expression profiles were obtained from the cancer genome atlas (TCGA) database. For each MRI study, necrotic, enhancing tumor, and edematous regions were segmented by an expert. A total of 30 radiomic descriptors (i.e. Haralick, Laws energy, Gabor) were extracted from every region across all three MRI protocols. By performing unsupervised clustering of the expression profile of hypoxia associated genes, a "low", "medium", or "high" index was defined for every study. Spearman correlation was then used to identify the most significantly correlated MRI features with the hypoxia index for every study. These features were further used to categorize each study as STS, MTS, and LTS using Kaplan-Meier (KM) analysis. Our results revealed that the most significant features (p < 0.05) were identified as Laws energy and Haralick features that capture image heterogeneity on FLAIR and Gd-T1w sequences. We also found these radiomic features to be significantly associated with survival, distinguishing MTS from LTS (p=.005) and STS from LTS (p=.0008).

The Maize PI/GLO Ortholog Zmm16/sterile tassel silky ear1 Interacts with the Zygomorphy and Sex Determination Pathways in Flower Development.

PubMed

Bartlett, Madelaine E; Williams, Steven K; Taylor, Zac; DeBlasio, Stacy; Goldshmidt, Alexander; Hall, Darren H; Schmidt, Robert J; Jackson, David P; Whipple, Clinton J

2015-11-01

In monocots and eudicots, B class function specifies second and third whorl floral organ identity as described in the classic ABCE model. Grass B class APETALA3/DEFICIENS orthologs have been functionally characterized; here, we describe the positional cloning and characterization of a maize (Zea mays) PISTILLATA/GLOBOSA ortholog Zea mays mads16 (Zmm16)/sterile tassel silky ear1 (sts1). We show that, similar to many eudicots, all the maize B class proteins bind DNA as obligate heterodimers and positively regulate their own expression. However, sts1 mutants have novel phenotypes that provide insight into two derived aspects of maize flower development: carpel abortion and floral asymmetry. Specifically, we show that carpel abortion acts downstream of organ identity and requires the growth-promoting factor grassy tillers1 and that the maize B class genes are expressed asymmetrically, likely in response to zygomorphy of grass floral primordia. Further investigation reveals that floral phyllotactic patterning is also zygomorphic, suggesting significant mechanistic differences with the well-characterized models of floral polarity. These unexpected results show that despite extensive study of B class gene functions in diverse flowering plants, novel insights can be gained from careful investigation of homeotic mutants outside the core eudicot model species. © 2015 American Society of Plant Biologists. All rights reserved.

Functional integration of the posterior superior temporal sulcus correlates with facial expression recognition.

PubMed

Wang, Xu; Song, Yiying; Zhen, Zonglei; Liu, Jia

2016-05-01

Face perception is essential for daily and social activities. Neuroimaging studies have revealed a distributed face network (FN) consisting of multiple regions that exhibit preferential responses to invariant or changeable facial information. However, our understanding about how these regions work collaboratively to facilitate facial information processing is limited. Here, we focused on changeable facial information processing, and investigated how the functional integration of the FN is related to the performance of facial expression recognition. To do so, we first defined the FN as voxels that responded more strongly to faces than objects, and then used a voxel-based global brain connectivity method based on resting-state fMRI to characterize the within-network connectivity (WNC) of each voxel in the FN. By relating the WNC and performance in the "Reading the Mind in the Eyes" Test across participants, we found that individuals with stronger WNC in the right posterior superior temporal sulcus (rpSTS) were better at recognizing facial expressions. Further, the resting-state functional connectivity (FC) between the rpSTS and right occipital face area (rOFA), early visual cortex (EVC), and bilateral STS were positively correlated with the ability of facial expression recognition, and the FCs of EVC-pSTS and OFA-pSTS contributed independently to facial expression recognition. In short, our study highlights the behavioral significance of intrinsic functional integration of the FN in facial expression processing, and provides evidence for the hub-like role of the rpSTS for facial expression recognition. Hum Brain Mapp 37:1930-1940, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Spaceflight Transcriptomes: Unique Responses to a Novel Environment

PubMed Central

Paul, Anna-Lisa; Zupanska, Agata K.; Ostrow, Dejerianne T.; Zhang, Yanping; Sun, Yijun; Li, Jian-Liang; Shanker, Savita; Farmerie, William G.; Amalfitano, Claire E.

2012-01-01

Abstract The spaceflight environment presents unique challenges to terrestrial biology, including but not limited to the direct effects of gravity. As we near the end of the Space Shuttle era, there remain fundamental questions about the response and adaptation of plants to orbital spaceflight conditions. We address a key baseline question of whether gene expression changes are induced by the orbital environment, and then we ask whether undifferentiated cells, cells presumably lacking the typical gravity response mechanisms, perceive spaceflight. Arabidopsis seedlings and undifferentiated cultured Arabidopsis cells were launched in April, 2010, as part of the BRIC-16 flight experiment on STS-131. Biologically replicated DNA microarray and averaged RNA digital transcript profiling revealed several hundred genes in seedlings and cell cultures that were significantly affected by launch and spaceflight. The response was moderate in seedlings; only a few genes were induced by more than 7-fold, and the overall intrinsic expression level for most differentially expressed genes was low. In contrast, cell cultures displayed a more dramatic response, with dozens of genes showing this level of differential expression, a list comprised primarily of heat shock–related and stress-related genes. This baseline transcriptome profiling of seedlings and cultured cells confirms the fundamental hypothesis that survival of the spaceflight environment requires adaptive changes that are both governed and displayed by alterations in gene expression. The comparison of intact plants with cultures of undifferentiated cells confirms a second hypothesis: undifferentiated cells can detect spaceflight in the absence of specialized tissue or organized developmental structures known to detect gravity. Key Words: Tissue culture—Microgravity—Low Earth orbit—Space Shuttle—Microarray. Astrobiology 12, 40–56. PMID:22221117

A resource of large-scale molecular markers for monitoring Agropyron cristatum chromatin introgression in wheat background based on transcriptome sequences.

PubMed

Zhang, Jinpeng; Liu, Weihua; Lu, Yuqing; Liu, Qunxing; Yang, Xinming; Li, Xiuquan; Li, Lihui

2017-09-20

Agropyron cristatum is a wild grass of the tribe Triticeae and serves as a gene donor for wheat improvement. However, very few markers can be used to monitor A. cristatum chromatin introgressions in wheat. Here, we reported a resource of large-scale molecular markers for tracking alien introgressions in wheat based on transcriptome sequences. By aligning A. cristatum unigenes with the Chinese Spring reference genome sequences, we designed 9602 A. cristatum expressed sequence tag-sequence-tagged site (EST-STS) markers for PCR amplification and experimental screening. As a result, 6063 polymorphic EST-STS markers were specific for the A. cristatum P genome in the single-receipt wheat background. A total of 4956 randomly selected polymorphic EST-STS markers were further tested in eight wheat variety backgrounds, and 3070 markers displaying stable and polymorphic amplification were validated. These markers covered more than 98% of the A. cristatum genome, and the marker distribution density was approximately 1.28 cM. An application case of all EST-STS markers was validated on the A. cristatum 6 P chromosome. These markers were successfully applied in the tracking of alien A. cristatum chromatin. Altogether, this study provided a universal method of large-scale molecular marker development to monitor wild relative chromatin in wheat.

Determining Clostridium difficile intra-taxa diversity by mining multilocus sequence typing databases.

PubMed

Muñoz, Marina; Ríos-Chaparro, Dora Inés; Patarroyo, Manuel Alfonso; Ramírez, Juan David

2017-03-14

Multilocus sequence typing (MLST) is a highly discriminatory typing strategy; it is reproducible and scalable. There is a MLST scheme for Clostridium difficile (CD), a gram positive bacillus causing different pathologies of the gastrointestinal tract. This work was aimed at describing the frequency of sequence types (STs) and Clades (C) reported and evalute the intra-taxa diversity in the CD MLST database (CD-MLST-db) using an MLSA approach. Analysis of 1778 available isolates showed that clade 1 (C1) was the most frequent worldwide (57.7%), followed by C2 (29.1%). Regarding sequence types (STs), it was found that ST-1, belonging to C2, was the most frequent. The isolates analysed came from 17 countries, mostly from the United Kingdom (UK) (1541 STs, 87.0%). The diversity of the seven housekeeping genes in the MLST scheme was evaluated, and alleles from the profiles (STs), for identifying CD population structure. It was found that adk and atpA are conserved genes allowing a limited amount of clusters to be discriminated; however, different genes such as drx, glyA and particularly sodA showed high diversity indexes and grouped CD populations in many clusters, suggesting that these genes' contribution to CD typing should be revised. It was identified that CD STs reported to date have a mostly clonal population structure with foreseen events of recombination; however, one group of STs was not assigned to a clade being highly different containing at least nine well-supported clusters, suggesting a greater amount of clades for CD. This study shows the usefulness of CD-MLST-db as a tool for studying CD distribution and population structure, identifying the need for reviewing the usefulness of sodA as housekeeping gene within the MLST scheme and suggesting the existence of a greater amount of CD clades. The study also shows the plausible exchange of genetic material between STs, contributing towards intra-taxa genetic diversity.

Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments.

PubMed

Liu, Juanxu; Li, Jingyu; Wang, Huinan; Fu, Zhaodi; Liu, Juan; Yu, Yixun

2011-01-01

Ethylene-responsive element-binding factor (ERF) genes constitute one of the largest transcription factor gene families in plants. In Arabidopsis and rice, only a few ERF genes have been characterized so far. Flower senescence is associated with increased ethylene production in many flowers. However, the characterization of ERF genes in flower senescence has not been reported. In this study, 13 ERF cDNAs were cloned from petunia. Based on the sequence characterization, these PhERFs could be classified into four of the 12 known ERF families. Their predicted amino acid sequences exhibited similarities to ERFs from other plant species. Expression analyses of PhERF mRNAs were performed in corollas and gynoecia of petunia flower. The 13 PhERF genes displayed differential expression patterns and levels during natural flower senescence. Exogenous ethylene accelerates the transcription of the various PhERF genes, and silver thiosulphate (STS) decreased the transcription of several PhERF genes in corollas and gynoecia. PhERF genes of group VII showed a strong association with the rise in ethylene production in both petals and gynoecia, and might be associated particularly with flower senescence in petunia. The effect of sugar, methyl jasmonate, and the plant hormones abscisic acid, salicylic acid, and 6-benzyladenine in regulating the different PhERF transcripts was investigated. Functional nuclear localization signal analyses of two PhERF proteins (PhERF2 and PhERF3) were carried out using fluorescence microscopy. These results supported a role for petunia PhERF genes in transcriptional regulation of petunia flower senescence processes.

Face-selective regions differ in their ability to classify facial expressions

PubMed Central

Zhang, Hui; Japee, Shruti; Nolan, Rachel; Chu, Carlton; Liu, Ning; Ungerleider, Leslie G

2016-01-01

Recognition of facial expressions is crucial for effective social interactions. Yet, the extent to which the various face-selective regions in the human brain classify different facial expressions remains unclear. We used functional magnetic resonance imaging (fMRI) and support vector machine pattern classification analysis to determine how well face-selective brain regions are able to decode different categories of facial expression. Subjects participated in a slow event-related fMRI experiment in which they were shown 32 face pictures, portraying four different expressions: neutral, fearful, angry, and happy and belonging to eight different identities. Our results showed that only the amygdala and the posterior superior temporal sulcus (STS) were able to accurately discriminate between these expressions, albeit in different ways: The amygdala discriminated fearful faces from non-fearful faces, whereas STS discriminated neutral from emotional (fearful, angry and happy) faces. In contrast to these findings on the classification of emotional expression, only the fusiform face area (FFA) and anterior inferior temporal cortex (aIT) could discriminate among the various facial identities. Further, the amygdala and STS were better than FFA and aIT at classifying expression, while FFA and aIT were better than the amygdala and STS at classifying identity. Taken together, our findings indicate that the decoding of facial emotion and facial identity occurs in different neural substrates: the amygdala and STS for the former and FFA and aIT for the latter. PMID:26826513

Face-selective regions differ in their ability to classify facial expressions.

PubMed

Zhang, Hui; Japee, Shruti; Nolan, Rachel; Chu, Carlton; Liu, Ning; Ungerleider, Leslie G

2016-04-15

Recognition of facial expressions is crucial for effective social interactions. Yet, the extent to which the various face-selective regions in the human brain classify different facial expressions remains unclear. We used functional magnetic resonance imaging (fMRI) and support vector machine pattern classification analysis to determine how well face-selective brain regions are able to decode different categories of facial expression. Subjects participated in a slow event-related fMRI experiment in which they were shown 32 face pictures, portraying four different expressions: neutral, fearful, angry, and happy and belonging to eight different identities. Our results showed that only the amygdala and the posterior superior temporal sulcus (STS) were able to accurately discriminate between these expressions, albeit in different ways: the amygdala discriminated fearful faces from non-fearful faces, whereas STS discriminated neutral from emotional (fearful, angry and happy) faces. In contrast to these findings on the classification of emotional expression, only the fusiform face area (FFA) and anterior inferior temporal cortex (aIT) could discriminate among the various facial identities. Further, the amygdala and STS were better than FFA and aIT at classifying expression, while FFA and aIT were better than the amygdala and STS at classifying identity. Taken together, our findings indicate that the decoding of facial emotion and facial identity occurs in different neural substrates: the amygdala and STS for the former and FFA and aIT for the latter. Published by Elsevier Inc.

Expression of Enzymes that Metabolize Medications

NASA Technical Reports Server (NTRS)

Wotring, V. E.; Peters, C. P.

2011-01-01

INTRODUCTION: Increased exposure to radiation is one physiological stressor associated with spaceflight and it is feasible to conduct ground experiments using known radiation exposures. The health of the liver, especially the activity rate of its metabolic enzymes, determines the concentration of circulating drugs as well as the duration of their efficacy. While radiation is known to alter normal physiological function, how radiation affects liver metabolism of administered medications is unclear. Crew health could be affected if the actions of medications used in spaceflight deviated from expectations formed during terrestrial medication use. This study is an effort to identify liver metabolic enzymes whose expression is altered by spaceflight or by radiation exposures that mimic features of the spaceflight environment. METHODS: Using procedures approved by the Animal Care and Use Committee, mice were exposed to either 137Cs (controls, 50 mGy, 6Gy, or 50 mGy + 6Gy separated by 24 hours) or 13 days of spaceflight on STS 135. Animals were anesthetized and sacrificed at several time points (4 hours, 24 hours or 7 days) after their last radiation exposure, or within 6 hours of return to Earth for the STS 135 animals. Livers were removed immediately and flash-frozen in liquid nitrogen. Tissue was homogenized, RNA extracted, purified and quality-tested. Complementary DNA was prepared from high-quality RNA samples, and used in RT-qPCR experiments to determine relative expression of a wide variety of genes involved in general metabolism and drug metabolism. RESULTS: Results of the ground radiation exposure experiments indicated 65 genes of the 190 tested were significantly affected by at least one of the radiation doses. Many of the affected genes are involved in the metabolism of drugs with hydrophobic or steroid-like structures, maintenance of redox homeostasis and repair of DNA damage. Most affected genes returned to near control expression levels by 7 days post-treatment. Not all recovered completely by the final time point tested: with 6 Gy exposure, metallothionein expression was 132-fold more than control at the 4 hr time point, and fell at each later time point (11-fold at 24 hrs, and 8-fold at 7 days). In contrast, there were other genes whose expression was altered and remained relatively constant through the 7 day period we tested. One examples is Cyp17a1, which showed a 4-fold elevation at 4 hrs after exposure and remained constant for 7 days after the last treatment. Spaceflight samples evaluated with similar methods and comparisons will be made between the radiation-treated groups and the spaceflight samples. CONCLUSION It seems likely that radiation exposure triggers homeostatic mechanisms, which could include alterations of gene expression. Better understanding of these pathways could aid in optimizing medications doses given to crewmembers who require treatment and eventually, to development of new countermeasures to ameliorate or prevent radiation-induced damage to cells and tissues.

Transgene Expression Patterns Indicate That Spaceflight Affects Stress Signal Perception and Transduction in Arabidopsis1

PubMed Central

Paul, Anna-Lisa; Daugherty, Christine J.; Bihn, Elizabeth A.; Chapman, David K.; Norwood, Kelly L.L.; Ferl, Robert J.

2001-01-01

The use of plants as integral components of life support systems remains a cornerstone of strategies for long-term human habitation of space and extraterrestrial colonization. Spaceflight experiments over the past few decades have refined the hardware required to grow plants in low-earth orbit and have illuminated fundamental issues regarding spaceflight effects on plant growth and development. Potential incipient hypoxia, resulting from the lack of convection-driven gas movement, has emerged as a possible major impact of microgravity. We developed transgenic Arabidopsis containing the alcohol dehydrogenase (Adh) gene promoter linked to the β-glucuronidase (GUS) reporter gene to address specifically the possibility that spaceflight induces the plant hypoxia response and to assess whether any spaceflight response was similar to control terrestrial hypoxia-induced gene expression patterns. The staining patterns resulting from a 5-d mission on the orbiter Columbia during mission STS-93 indicate that the Adh/GUS reporter gene was activated in roots during the flight. However, the patterns of expression were not identical to terrestrial control inductions. Moreover, although terrestrial hypoxia induces Adh/GUS expression in the shoot apex, no apex staining was observed in the spaceflight plants. This indicates that either the normal hypoxia response signaling is impaired in spaceflight or that spaceflight inappropriately induces Adh/GUS activity for reasons other than hypoxia. PMID:11402191

Phylogenetic diversity, antimicrobial susceptibility and virulence gene profiles of Brachyspira hyodysenteriae isolates from pigs in Germany

PubMed Central

Joerling, Jessica; Barth, Stefanie A.; Schlez, Karen; Willems, Hermann

2018-01-01

Swine dysentery (SD) is an economically important diarrheal disease in pigs caused by different strongly hemolytic Brachyspira (B.) species, such as B. hyodysenteriae, B. suanatina and B. hampsonii. Possible associations of epidemiologic data, such as multilocus sequence types (STs) to virulence gene profiles and antimicrobial susceptibility are rather scarce, particularly for B. hyodysenteriae isolates from Germany. In this study, B. hyodysenteriae (n = 116) isolated from diarrheic pigs between 1990 and 2016 in Germany were investigated for their STs, susceptibility to the major drugs used for treatment of SD (tiamulin and valnemulin) and genes that were previously linked with virulence and encode for hemolysins (tlyA, tlyB, tlyC, hlyA, BHWA1_RS02885, BHWA1_RS09085, BHWA1_RS04705, and BHWA1_RS02195), outer membrane proteins (OMPs) (bhlp16, bhlp17.6, bhlp29.7, bhmp39f, and bhmp39h) as well as iron acquisition factors (ftnA and bitC). Multilocus sequence typing (MLST) revealed that 79.4% of the isolates belonged to only three STs, namely ST52 (41.4%), ST8 (12.1%), and ST112 (25.9%) which have been observed in other European countries before. Another 24 isolates belonged to twelve new STs (ST113-118, ST120-123, ST131, and ST193). The temporal distribution of STs revealed the presence of new STs as well as the regular presence of ST52 over three decades (1990s–2000s). The proportion of strains that showed resistance to both tiamulin und valnemulin (39.1%) varied considerably among the most frequent STs ranging from 0% (0/14 isolates resistant) in ST8 isolates to 46.7% (14/30), 52.1% (25/48), and 85.7% (6/7) in isolates belonging to ST112, ST52, and ST114, respectively. All hemolysin genes as well as the iron-related gene ftnA and the OMP gene bhlp29.7 were regularly present in the isolates, while the OMP genes bhlp17.6 and bhmp39h could not be detected. Sequence analysis of hemolysin genes of selected isolates revealed co-evolution of tlyB, BHWA1_RS02885, BHWA1_RS09085, and BHWA1_RS02195 with the core genome and suggested independent evolution of tlyA, tlyC, and hlyA. Our data indicate that in Germany, swine dysentery might be caused by a limited number of B. hyodysenteriae clonal groups. Major STs (ST8, ST52, and ST112) are shared with other countries in Europe suggesting a possible role of the European intra-Community trade of pigs in the dissemination of certain clones. The identification of several novel STs, some of which are single or double locus variants of ST52, may on the other hand hint towards an ongoing diversification of the pathogen in the studied area. The linkage of pleuromutilin susceptibility and sequence type of an isolate might reflect a clonal expansion of the underlying resistance mechanism, namely mutations in the ribosomal RNA genes. A linkage between single virulence-associated genes (VAGs) or even VAG patterns and the phylogenetic background of the isolates could not be established, since almost all VAGs were regularly present in the isolates. PMID:29324785

Individual Variation in Incentive Salience Attribution and Accumbens Dopamine Transporter Expression and Function

PubMed Central

Singer, Bryan F.; Guptaroy, Bipasha; Austin, Curtis J.; Wohl, Isabella; Lovic, Vedran; Seiler, Jillian L; Vaughan, Roxanne A.; Gnegy, Margaret E.; Robinson, Terry E.; Aragona, Brandon J.

2015-01-01

Cues (conditioned stimuli; CSs) associated with rewards can come to motivate behavior, but there is considerable individual variation in their ability to do so. For example, a lever-CS that predicts food reward becomes attractive, wanted, and elicits reward-seeking behavior to a greater extent in some rats (“sign-trackers”; STs), than others (“goal-trackers”; GTs). Variation in dopamine (DA) neurotransmission in the nucleus accumbens (NAc) core is thought to contribute to such individual variation. Given that the DA transporter (DAT) exerts powerful regulation over DA signaling, we characterized the expression and function of the DAT in the accumbens of STs and GTs. STs showed greater DAT surface expression in ventral striatal synaptosomes than GTs, and ex vivo fast-scan cyclic voltammetry recordings of electrically-evoked DA release confirmed enhanced DAT function in STs, as indicated by faster DA uptake, specifically in the NAc core. Consistent with this, systemic amphetamine (AMPH) produced greater inhibition of DA uptake in STs than in GTs. Furthermore, injection of AMPH directly into the NAc core enhanced lever-directed approach in STs, presumably by amplifying the incentive value of the CS, but had no effect on goal tracking behavior. On the other hand, there were no differences between STs and GTs in electrically-evoked DA release in slices, or in total ventral striatal DA content. We conclude that greater DAT surface expression may facilitate the attribution of incentive salience to discrete reward cues. Investigating this variability in animal sub-populations may help explain why some people abuse drugs, while others do not. PMID:26613374

Identification of a rice gene (Bph 1) conferring resistance to brown planthopper (Nilaparvata lugens Stal) using STS markers.

PubMed

Kim, Suk-Man; Sohn, Jae-Keun

2005-08-31

This study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (BPH) biotype 1, using japonica type resistant lines. Bulked segregant analyses were conducted using 520 RAPD primers to identify RAPD fragments linked to the BPH resistance gene. Eleven RAPDs were shown to be polymorphic amplicons between resistant and susceptible progeny. One of these primers, OPE 18, which amplified a 923 bp band tightly linked to resistance, was converted into a sequence-tagged-site (STS) marker. The STS marker, BpE18-3, was easily detectable as a dominant band with tight linkage (3.9cM) to Bph1. It promises to be useful as a marker for assisted selection of resistant progeny in backcross breeding programs to introgress the resistance gene into elite japonica cultivars.

Melatonin affects the dynamic steady-state equilibrium of estrogen sulfates in human umbilical vein endothelial cells by regulating the balance between estrogen sulfatase and sulfotransferase.

PubMed

González, Alicia; Martínez-Campa, Carlos; Alonso-González, Carolina; Cos, Samuel

2015-12-01

Melatonin is known to reduce the growth of endocrine-responsive breast cancers by interacting with estrogen signaling pathways. Estrogens play an important role in breast cancer, but also in various types of tissues, including vascular tissue. Estrogen sulfatase (STS) converts inactive estrogen sulfates into active estrogens, whereas estrogen sulfotransferase (EST) sulfonates estrogens to estrogen sulfates. Therefore, STS and EST are considered to be involved in the regulation of local estrogen levels in hormone‑dependent tumors and in non-pathologic tissues, such as those of the vascular system. Estrogens have a major impact on the vasculature, influencing vascular function, the expression of adhesion proteins, angiogenesis and the inflammatory state. In this study, we investigated the status of STS and EST in human umbilical vein endothelial cells (HUVECs) and the modulatory effects of melatonin. Both STS and EST were highly expressed in the HUVECs. The enzymatic activity correlated with the expression levels in these cells. Our findings also demonstrated that melatonin, at physiological concentrations, modulated the synthesis and transformation of biologically active estrogens in HUVECs through the inhibition of STS activity and expression, and the stimulation of EST activity and expression. Since melatonin decreased the STS levels and increased the EST levels, it modified the dynamic steady‑state equilibrium of estrogen sulfates by increasing the inactive estrogen levels and decreasing the active estrogen levels. Therefore, melatonin may modulate the known different biological actions of estrogens in endothelial cells, as well as in estrogen-dependent tumors and non-pathologic tissues.

Systemic Microgravity Response: Utilizing GeneLab to Develop Hypotheses for Spaceflight Risks

NASA Technical Reports Server (NTRS)

Beheshti, Afshin; Fogle, Homer; Galazka, Jonathan; Kidane, Yared; Chakravarty, Kaushik; Berrios, Daniel C.; Costes, Sylvain V.

2017-01-01

Biological risks associated with microgravity is a major concern for space travel. Although determination of risk has been a focus for NASA research, data examining systemic (i.e., multi- or pan-tissue) responses to space flight are sparse. The overall goal of our work is to identify potential master regulators responsible for such responses to microgravity conditions. To do this we utilized the NASA GeneLab database which contains a wide array of omics experiments, including data from: 1) different flight conditions (space shuttle (STS) missions vs. International Space Station (ISS); 2) different tissues; and 3) different types of assays that measure epigenetic, transcriptional, and protein expression changes. We have performed meta-analysis identifying potential master regulators involved with systemic responses to microgravity. The analysis used 7 different murine and rat data sets, examining the following tissues: liver, kidney, adrenal gland, thymus, mammary gland, skin, and skeletal muscle (soleus, extensor digitorum longus, tibialis anterior, quadriceps, and gastrocnemius). Using a systems biology approach, we were able to determine that p53 and immune related pathways appear central to pan-tissue microgravity responses. Evidence for a universal response in the form of consistency of change across tissues in regulatory pathways was observed in both STS and ISS experiments with varying durations; while degree of change in expression of these master regulators varied across species and strain, some change in these master regulators was universally observed. Interestingly, certain skeletal muscle (gastrocnemius and soleus) show an overall down-regulation in these genes, while in other types (extensor digitorum longus, tibialis anterior and quadriceps) they are up-regulated, suggesting certain muscle tissues may be compensating for atrophy responses caused by microgravity. Studying these organtissue-specific perturbations in molecular signaling networks, we demonstrate the value of GeneLab in characterizing potential master regulators associated with biological risks for spaceflight.

Comparative transcriptomics indicate changes in cell wall organization and stress response in seedlings during spaceflight.

PubMed

Johnson, Christina M; Subramanian, Aswati; Pattathil, Sivakumar; Correll, Melanie J; Kiss, John Z

2017-08-21

Plants will play an important role in the future of space exploration as part of bioregenerative life support. Thus, it is important to understand the effects of microgravity and spaceflight on gene expression in plant development. We analyzed the transcriptome of Arabidopsis thaliana using the Biological Research in Canisters (BRIC) hardware during Space Shuttle mission STS-131. The bioinformatics methods used included RMA (robust multi-array average), MAS5 (Microarray Suite 5.0), and PLIER (probe logarithmic intensity error estimation). Glycome profiling was used to analyze cell wall composition in the samples. In addition, our results were compared to those of two other groups using the same hardware on the same mission (BRIC-16). In our BRIC-16 experiments, we noted expression changes in genes involved in hypoxia and heat shock responses, DNA repair, and cell wall structure between spaceflight samples compared to the ground controls. In addition, glycome profiling supported our expression analyses in that there was a difference in cell wall components between ground control and spaceflight-grown plants. Comparing our studies to those of the other BRIC-16 experiments demonstrated that, even with the same hardware and similar biological materials, differences in results in gene expression were found among these spaceflight experiments. A common theme from our BRIC-16 space experiments and those of the other two groups was the downregulation of water stress response genes in spaceflight. In addition, all three studies found differential regulation of genes associated with cell wall remodeling and stress responses between spaceflight-grown and ground control plants. © 2017 Botanical Society of America.

Cultured Human Renal Cortical Cells

NASA Technical Reports Server (NTRS)

1998-01-01

During the STS-90 shuttle flight in April 1998, cultured renal cortical cells revealed new information about genes. Timothy Hammond, an investigator in NASA's microgravity biotechnology program was interested in culturing kidney tissue to study the expression of proteins useful in the treatment of kidney diseases. Protein expression is linked to the level of differentiation of the kidney cells, and Hammond had difficulty maintaining differentiated cells in vitro. Intrigued by the improvement in cell differentiation that he observed in rat renal cells cultured in NASA's rotating wall vessel (a bioreactor that simulates some aspects of microgravity) and during an experiment performed on the Russian Space Station Mir, Hammond decided to sleuth out which genes were responsible for controlling differentiation of kidney cells. To do this, he compared the gene activity of human renal cells in a variety of gravitational environments, including the microgravity of the space shuttle and the high-gravity environment of a centrifuge. Hammond found that 1,632 genes out of 10,000 analyzed changed their activity level in microgravity, more than in any of the other environments. These results have important implications for kidney research as well as for understanding the basic mechanism for controlling cell differentiation.

Microgravity

NASA Image and Video Library

1998-04-01

During the STS-90 shuttle flight in April 1998, cultured renal cortical cells revealed new information about genes. Timothy Hammond, an investigator in NASA's microgravity biotechnology program was interested in culturing kidney tissue to study the expression of proteins useful in the treatment of kidney diseases. Protein expression is linked to the level of differentiation of the kidney cells, and Hammond had difficulty maintaining differentiated cells in vitro. Intrigued by the improvement in cell differentiation that he observed in rat renal cells cultured in NASA's rotating wall vessel (a bioreactor that simulates some aspects of microgravity) and during an experiment performed on the Russian Space Station Mir, Hammond decided to sleuth out which genes were responsible for controlling differentiation of kidney cells. To do this, he compared the gene activity of human renal cells in a variety of gravitational environments, including the microgravity of the space shuttle and the high-gravity environment of a centrifuge. Hammond found that 1,632 genes out of 10,000 analyzed changed their activity level in microgravity, more than in any of the other environments. These results have important implications for kidney research as well as for understanding the basic mechanism for controlling cell differentiation.

Individual variation in incentive salience attribution and accumbens dopamine transporter expression and function.

PubMed

Singer, Bryan F; Guptaroy, Bipasha; Austin, Curtis J; Wohl, Isabella; Lovic, Vedran; Seiler, Jillian L; Vaughan, Roxanne A; Gnegy, Margaret E; Robinson, Terry E; Aragona, Brandon J

2016-03-01

Cues (conditioned stimuli; CSs) associated with rewards can come to motivate behavior, but there is considerable individual variation in their ability to do so. For example, a lever-CS that predicts food reward becomes attractive and wanted, and elicits reward-seeking behavior, to a greater extent in some rats ('sign-trackers'; STs) than others ('goal-trackers'; GTs). Variation in dopamine (DA) neurotransmission in the nucleus accumbens (NAc) core is thought to contribute to such individual variation. Given that the DA transporter (DAT) exerts powerful regulation over DA signaling, we characterized the expression and function of the DAT in the accumbens of STs and GTs. STs showed greater DAT surface expression in ventral striatal synaptosomes than GTs, and ex vivo fast-scan cyclic voltammetry recordings of electrically evoked DA release confirmed enhanced DAT function in STs, as indicated by faster DA uptake, specifically in the NAc core. Consistent with this, systemic amphetamine (AMPH) produced greater inhibition of DA uptake in STs than in GTs. Furthermore, injection of AMPH directly into the NAc core enhanced lever-directed approach in STs, presumably by amplifying the incentive value of the CS, but had no effect on goal-tracking behavior. On the other hand, there were no differences between STs and GTs in electrically-evoked DA release in slices, or in total ventral striatal DA content. We conclude that greater DAT surface expression may facilitate the attribution of incentive salience to discrete reward cues. Investigating this variability in animal sub-populations may help explain why some people abuse drugs while others do not. © 2015 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.

Molecular Epidemiologic Analysis of Enterococcus faecalis Isolates in Cuba by Multilocus Sequence Typing

PubMed Central

Kobayashi, Nobumichi; Nagashima, Shigeo

2009-01-01

We carried out the first study of Enterococcus faecalis clinical isolates in Cuba by multilocus sequence typing linking the molecular typing data with the presence of virulence determinants and the antibiotic resistance genes. A total of 23 E. faecalis isolates recovered from several clinic sources and geographic areas of Cuba during a period between 2000 and 2005 were typed by multilocus sequence typing. Thirteen sequence types (STs) including five novel STs were identified, and the ST 64 (clonal complex [CC] 8), ST 6 (CC2), ST 21(CC21), and ST 16 (CC58) were found in more than one strain. Sixty-seven percent of STs corresponded to STs reported previously in Spain, Poland, and The Netherlands, and other STs (ST115, ST64, ST6, and ST40) were genetically close to those detected in the United States. Prevalence of both antimicrobial resistance genes [aac(6′)-aph(2″), aph(3′), ant(6), ant(3″)(9), aph(2″)-Id, aph(2″)-Ic, erm(B), erm(A), erm(C), mef(A), tet(M), and tet(L)] and virulence genes (agg, gelE, cylA, esp, ccf, and efaAfs) were examined by polymerase chain reaction. Aminoglycoside resistance genes aac(6′)-Ie-aph(2″)-Ia, aph(3′), ant(6), ant(3″)(9) were more frequently detected in ST6, ST16, ST23, ST64, and ST115. The multidrug resistance was distributed to all STs detected, except for ST117 and singleton ST225. The presence of cyl gene was specifically linked to the ST64 and ST16. Presence of the esp, gel, and agg genes was not specific to any particular ST. This research provided the first insight into the population structure of E. faecalis in Cuba, that is, most Cuban strains were related to European strains, whereas others to U.S. strains. The CC2, CC21, and CC8, three of the biggest CCs in the world, were evidently circulating in Cuba, associated with multidrug resistance and virulence traits. PMID:19857135

Estrone Sulfate Transport and Steroid Sulfatase Activity in Colorectal Cancer: Implications for Hormone Replacement Therapy.

PubMed

Gilligan, Lorna C; Gondal, Ali; Tang, Vivien; Hussain, Maryam T; Arvaniti, Anastasia; Hewitt, Anne-Marie; Foster, Paul A

2017-01-01

Hormone replacement therapy (HRT) affects the incidence and potential progression of colorectal cancer (CRC). As HRT primarily consists of estrone sulfate (E 1 S), understanding whether this conjugated estrogen is transported and metabolized in CRC will define its potential effect in this malignancy. Here, we show that a panel of CRC cell lines (Colo205, Caco2, HCT116, HT-29) have steroid sulfatase (STS) activity, and thus can hydrolyze E 1 S. STS activity is significantly higher in CRC cell lysate, suggesting the importance of E 1 S transport in intracellular STS substrate availability. As E 1 S transport is regulated by the expression pattern of certain solute carrier organic anion transporter polypeptides, we show that in CRC OATP4A1 is the most abundantly expressed transporter. All four CRC cell lines rapidly transported E 1 S into cells, with this effect significantly inhibited by the competitive OATP inhibitor BSP. Transient knockdown of OATP4A1 significantly disrupted E 1 S uptake. Examination of estrogen receptor status showed ERα was present in Colo205 and Caco2 cells. None of the cells expressed ERβ. Intriguingly, HCT116 and HT29 cells strongly expressed the G protein coupled estrogen receptor (GPER), and that stimulation of this receptor with estradiol (E 2 ) and G1, a GPER agonist, significantly ( p < 0.01) increased STS activity. Furthermore, tamoxifen and fulvestrant, known GPER agonist, also increased CRC STS activity, with this effect inhibited by the GPER antagonist G15. These results suggest that CRC can take up and hydrolyze E 1 S, and that subsequent GPER stimulation increases STS activity in a potentially novel positive feedback loop. As elevated STS expression is associated with poor prognosis in CRC, these results suggest HRT, tamoxifen and fulvestrant may negatively impact CRC patient outcomes.

The correlates of subjective perception of identity and expression in the face network: an fMRI adaptation study

PubMed Central

Fox, Christopher J.; Moon, So Young; Iaria, Giuseppe; Barton, Jason J.S.

2009-01-01

The recognition of facial identity and expression are distinct tasks, with current models hypothesizing anatomic segregation of processing within a face-processing network. Using fMRI adaptation and a region-of-interest approach, we assessed how the perception of identity and expression changes in morphed stimuli affected the signal within this network, by contrasting (a) changes that crossed categorical boundaries of identity or expression with those that did not, and (b) changes that subjects perceived as causing identity or expression to change, versus changes that they perceived as not affecting the category of identity or expression. The occipital face area (OFA) was sensitive to any structural change in a face, whether it was identity or expression, but its signal did not correlate with whether subjects perceived a change or not. Both the fusiform face area (FFA) and the posterior superior temporal sulcus (pSTS) showed release from adaptation when subjects perceived a change in either identity or expression, although in the pSTS this effect only occurred when subjects were explicitly attending to expression. The middle superior temporal sulcus (mSTS) showed release from adaptation for expression only, and the precuneus for identity only. The data support models where the OFA is involved in the early perception of facial structure. However, evidence for a functional overlap in the FFA and pSTS, with both identity and expression signals in both areas, argues against a complete independence of identity and expression processing in these regions of the core face-processing network. PMID:18852053

The correlates of subjective perception of identity and expression in the face network: an fMRI adaptation study.

PubMed

Fox, Christopher J; Moon, So Young; Iaria, Giuseppe; Barton, Jason J S

2009-01-15

The recognition of facial identity and expression are distinct tasks, with current models hypothesizing anatomic segregation of processing within a face-processing network. Using fMRI adaptation and a region-of-interest approach, we assessed how the perception of identity and expression changes in morphed stimuli affected the signal within this network, by contrasting (a) changes that crossed categorical boundaries of identity or expression with those that did not, and (b) changes that subjects perceived as causing identity or expression to change, versus changes that they perceived as not affecting the category of identity or expression. The occipital face area (OFA) was sensitive to any structural change in a face, whether it was identity or expression, but its signal did not correlate with whether subjects perceived a change or not. Both the fusiform face area (FFA) and the posterior superior temporal sulcus (pSTS) showed release from adaptation when subjects perceived a change in either identity or expression, although in the pSTS this effect only occurred when subjects were explicitly attending to expression. The middle superior temporal sulcus (mSTS) showed release from adaptation for expression only, and the precuneus for identity only. The data support models where the OFA is involved in the early perception of facial structure. However, evidence for a functional overlap in the FFA and pSTS, with both identity and expression signals in both areas, argues against a complete independence of identity and expression processing in these regions of the core face-processing network.

MEG demonstrates a supra-additive response to facial and vocal emotion in the right superior temporal sulcus.

PubMed

Hagan, Cindy C; Woods, Will; Johnson, Sam; Calder, Andrew J; Green, Gary G R; Young, Andrew W

2009-11-24

An influential neural model of face perception suggests that the posterior superior temporal sulcus (STS) is sensitive to those aspects of faces that produce transient visual changes, including facial expression. Other researchers note that recognition of expression involves multiple sensory modalities and suggest that the STS also may respond to crossmodal facial signals that change transiently. Indeed, many studies of audiovisual (AV) speech perception show STS involvement in AV speech integration. Here we examine whether these findings extend to AV emotion. We used magnetoencephalography to measure the neural responses of participants as they viewed and heard emotionally congruent fear and minimally congruent neutral face and voice stimuli. We demonstrate significant supra-additive responses (i.e., where AV > [unimodal auditory + unimodal visual]) in the posterior STS within the first 250 ms for emotionally congruent AV stimuli. These findings show a role for the STS in processing crossmodal emotive signals.

Staurosporine synergistically potentiates the deoxycholate-mediated induction of COX-2 expression.

PubMed

Saeki, Tohru; Inui, Haruka; Fujioka, Saya; Fukuda, Suguru; Nomura, Ayumi; Nakamura, Yasushi; Park, Eun Young; Sato, Kenji; Kanamoto, Ryuhei

2014-08-01

Colorectal cancer is a major cause of cancer-related death in western countries, and thus there is an urgent need to elucidate the mechanism of colorectal tumorigenesis. A diet that is rich in fat increases the risk of colorectal tumorigenesis. Bile acids, which are secreted in response to the ingestion of fat, have been shown to increase the risk of colorectal tumors. The expression of cyclooxygenase (COX)-2, an inducible isozyme of cyclooxygenase, is induced by bile acids and correlates with the incidence and progression of cancers. In this study, we investigated the signal transduction pathways involved in the bile-acid-mediated induction of COX-2 expression. We found that staurosporine (sts), a potent protein kinase C (PKC) inhibitor, synergistically potentiated the deoxycholate-mediated induction of COX-2 expression. Sts did not increase the stabilization of COX-2 mRNA. The sts- and deoxycholate-mediated synergistic induction of COX-2 expression was suppressed by a membrane-permeable Ca(2+) chelator, a phosphoinositide 3-kinase inhibitor, a nuclear factor-κB pathway inhibitor, and inhibitors of canonical and stress-inducible mitogen-activated protein kinase pathways. Inhibition was also observed using PKC inhibitors, suggesting the involvement of certain PKC isozymes (η, θ, ι, ζ, or μ). Our results indicate that sts exerts its potentiating effects via the phosphorylation of p38. However, the effects of anisomycin did not mimic those of sts, indicating that although p38 activation is required, it does not enhance deoxycholate-induced COX-2 expression. We conclude that staurosporine synergistically enhances deoxycholate-induced COX-2 expression in RCM-1 colon cancer cells. © 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Heightened amygdala responsiveness in s-carriers of 5-HTTLPR genetic polymorphism reflects enhanced cortical rather than subcortical inputs: An MEG study.

PubMed

Luo, Qian; Holroyd, Tom; Mitchell, Derek; Yu, Henry; Cheng, Xi; Hodgkinson, Colin; Chen, Gang; McCaffrey, Daniel; Goldman, David; Blair, R James

2017-09-01

Short allele carriers (S-carriers) of the serotonin transporter gene (5-HTTLPR) show an elevated amygdala response to emotional stimuli relative to long allele carriers (LL-homozygous). However, whether this reflects increased responsiveness of the amygdala generally or interactions between the amygdala and the specific input systems remains unknown. It is argued that the amygdala receives input via a quick subcortical and a slower cortical pathway. If the elevated amygdala response in S-carriers reflects generally increased amygdala responding, then group differences in amygdala should be seen across the amygdala response time course. However, if the difference is a secondary consequence of enhanced amygdala-cortical interactions, then group differences might only be present later in the amygdala response. Using magnetoencephalography (MEG), we found an enhanced amygdala response to fearful expressions starting 40-50 ms poststimulus. However, group differences in the amygdala were only seen 190-200 ms poststimulus, preceded by increased superior temporal sulcus (STS) responses in S-carriers from 130 to 140 ms poststimulus. An enhanced amygdala response to angry expressions started 260-270 ms poststimulus with group differences in the amygdala starting at 160-170 ms poststimulus onset, preceded by increased STS responses in S-carriers from 150 to 160 ms poststimulus. These suggest that enhanced amygdala responses in S-carriers might reflect enhanced STS-amygdala connectivity in S-carriers. Hum Brain Mapp 38:4313-4321, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Multilocus Sequence Typing and Virulence-Associated Gene Profile Analysis of Staphylococcus aureus Isolates From Retail Ready-to-Eat Food in China.

PubMed

Yang, Xiaojuan; Yu, Shubo; Wu, Qingping; Zhang, Jumei; Wu, Shi; Rong, Dongli

2018-01-01

The aim of this study was to characterize the subtypes and virulence profiles of 69 Staphylococcus aureus isolates obtained from retail ready-to-eat food in China. The isolates were analyzed using multilocus sequence typing (MLST) and polymerase chain reaction (PCR) analysis of important virulence factor genes, including the staphylococcal enterotoxin (SE) genes ( sea , seb , sec , sed , see , seg , seh , sei , sej ), the exfoliative toxin genes ( eta and etb ), the toxic shock syndrome toxin-1 gene ( tst ), and the Panton-Valentine leucocidin-encoding gene ( pvl ). The isolates encompassed 26 different sequence types (STs), including four new STs (ST3482, ST3484, ST3485, ST3504), clustered in three clonal complexes and 17 singletons. The most prevalent STs were ST1, ST6, and ST15, constituting 34.8% of all isolates. Most STs (15/26, 57.7%) detected have previously been associated with human infections. All 13 toxin genes examined were detected in the S. aureus isolates, with 84.1% of isolates containing toxin genes. The three most prevalent toxin genes were seb (36.2%), sea (33.3%), and seg (33.3%). The classical SE genes ( sea - see ), which contribute significantly to staphylococcal food poisoning (SFP), were detected in 72.5% of the S. aureus isolates. In addition, pvl , eta , etb , and tst were found in 11.6, 10.1, 10.1, and 7.2% of the S. aureus isolates, respectively. Strains ST6 carrying sea and ST1 harboring sec-seh enterotoxin profile, which are the two most common clones associated with SFP, were also frequently detected in the food samples in this study. This study indicates that these S. aureus isolates present in Chinese ready-to-eat food represents a potential public health risk. These data are valuable for epidemiological studies, risk management, and public health strategies.

Multilocus Sequence Typing and Virulence-Associated Gene Profile Analysis of Staphylococcus aureus Isolates From Retail Ready-to-Eat Food in China

PubMed Central

Yang, Xiaojuan; Yu, Shubo; Wu, Qingping; Zhang, Jumei; Wu, Shi; Rong, Dongli

2018-01-01

The aim of this study was to characterize the subtypes and virulence profiles of 69 Staphylococcus aureus isolates obtained from retail ready-to-eat food in China. The isolates were analyzed using multilocus sequence typing (MLST) and polymerase chain reaction (PCR) analysis of important virulence factor genes, including the staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, sej), the exfoliative toxin genes (eta and etb), the toxic shock syndrome toxin-1 gene (tst), and the Panton-Valentine leucocidin-encoding gene (pvl). The isolates encompassed 26 different sequence types (STs), including four new STs (ST3482, ST3484, ST3485, ST3504), clustered in three clonal complexes and 17 singletons. The most prevalent STs were ST1, ST6, and ST15, constituting 34.8% of all isolates. Most STs (15/26, 57.7%) detected have previously been associated with human infections. All 13 toxin genes examined were detected in the S. aureus isolates, with 84.1% of isolates containing toxin genes. The three most prevalent toxin genes were seb (36.2%), sea (33.3%), and seg (33.3%). The classical SE genes (sea–see), which contribute significantly to staphylococcal food poisoning (SFP), were detected in 72.5% of the S. aureus isolates. In addition, pvl, eta, etb, and tst were found in 11.6, 10.1, 10.1, and 7.2% of the S. aureus isolates, respectively. Strains ST6 carrying sea and ST1 harboring sec-seh enterotoxin profile, which are the two most common clones associated with SFP, were also frequently detected in the food samples in this study. This study indicates that these S. aureus isolates present in Chinese ready-to-eat food represents a potential public health risk. These data are valuable for epidemiological studies, risk management, and public health strategies. PMID:29662467

Attention to emotion modulates fMRI activity in human right superior temporal sulcus.

PubMed

Narumoto, J; Okada, T; Sadato, N; Fukui, K; Yonekura, Y

2001-10-01

A parallel neural network has been proposed for processing various types of information conveyed by faces including emotion. Using functional magnetic resonance imaging (fMRI), we tested the effect of the explicit attention to the emotional expression of the faces on the neuronal activity of the face-responsive regions. Delayed match to sample procedure was adopted. Subjects were required to match the visually presented pictures with regard to the contour of the face pictures, facial identity, and emotional expressions by valence (happy and fearful expressions) and arousal (fearful and sad expressions). Contour matching of the non-face scrambled pictures was used as a control condition. The face-responsive regions that responded more to faces than to non-face stimuli were the bilateral lateral fusiform gyrus (LFG), the right superior temporal sulcus (STS), and the bilateral intraparietal sulcus (IPS). In these regions, general attention to the face enhanced the activities of the bilateral LFG, the right STS, and the left IPS compared with attention to the contour of the facial image. Selective attention to facial emotion specifically enhanced the activity of the right STS compared with attention to the face per se. The results suggest that the right STS region plays a special role in facial emotion recognition within distributed face-processing systems. This finding may support the notion that the STS is involved in social perception.

Multilocus sequence typing scheme for the Mycobacterium abscessus complex.

PubMed

Macheras, Edouard; Konjek, Julie; Roux, Anne-Laure; Thiberge, Jean-Michel; Bastian, Sylvaine; Leão, Sylvia Cardoso; Palaci, Moises; Sivadon-Tardy, Valérie; Gutierrez, Cristina; Richter, Elvira; Rüsch-Gerdes, Sabine; Pfyffer, Gaby E; Bodmer, Thomas; Jarlier, Vincent; Cambau, Emmanuelle; Brisse, Sylvain; Caro, Valérie; Rastogi, Nalin; Gaillard, Jean-Louis; Heym, Beate

2014-01-01

We developed a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus sensu lato, based on the partial sequencing of seven housekeeping genes: argH, cya, glpK, gnd, murC, pta and purH. This scheme was used to characterize a collection of 227 isolates recovered between 1994 and 2010 in France, Germany, Switzerland and Brazil. We identified 100 different sequence types (STs), which were distributed into three groups on the tree obtained by concatenating the sequences of the seven housekeeping gene fragments (3576bp): the M. abscessus sensu stricto group (44 STs), the "M. massiliense" group (31 STs) and the "M. bolletii" group (25 STs). SplitTree analysis showed a degree of intergroup lateral transfers. There was also evidence of lateral transfer events involving rpoB. The most prevalent STs in our collection were ST1 (CC5; 20 isolates) and ST23 (CC3; 31 isolates). Both STs were found in Europe and Brazil, and the latter was implicated in a large post-surgical procedure outbreak in Brazil. Respiratory isolates from patients with cystic fibrosis belonged to a large variety of STs; however, ST2 was predominant in this group of patients. Our MLST scheme, publicly available at www.pasteur.fr/mlst, offers investigators a valuable typing tool for M. abscessus sensu lato in future epidemiological studies throughout the world. Copyright © 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Genetic and Pharmacological Modulation of the Steroid Sulfatase Axis Improves Response Control; Comparison with Drugs Used in ADHD

PubMed Central

Davies, William; Humby, Trevor; Trent, Simon; Eddy, Jessica B; Ojarikre, Obah A; Wilkinson, Lawrence S

2014-01-01

Maladaptive response control is a feature of many neuropsychiatric conditions, including attention deficit hyperactivity disorder (ADHD). As ADHD is more commonly diagnosed in males than females, a pathogenic role for sex-linked genes has been suggested. Deletion or point mutation of the X-linked STS gene, encoding the enzyme steroid sulfatase (STS) influences risk for ADHD. We examined whether deletion of the Sts gene in the 39,XY*O mouse model, or pharmacological manipulation of the STS axis, via administration of the enzyme substrate dehydroepiandrosterone sulfate or the enzyme inhibitor COUMATE, influenced behavior in a novel murine analog of the stop-signal reaction time task used to detect inhibitory deficits in individuals with ADHD. Unexpectedly, both the genetic and pharmacological treatments resulted in enhanced response control, manifest as highly specific effects in the ability to cancel a prepotent action. For all three manipulations, the effect size was comparable to that seen with the commonly used ADHD therapeutics methylphenidate and atomoxetine. Hence, converging genetic and pharmacological evidence indicates that the STS axis is involved in inhibitory processes and can be manipulated to give rise to improvements in response control. While the precise neurobiological mechanism(s) underlying the effects remain to be established, there is the potential for exploiting this pathway in the treatment of disorders where failures in behavioral inhibition are prominent. PMID:24842408

Responses of Myosin Heavy Chain Phenotypes and Gene Expressions in Neck Muscle to Micro- an Hyper-Gravity in Mice

NASA Astrophysics Data System (ADS)

Ohira, Tomotaka; Ohira, Takashi; Kawano, F.; Shibaguchi, T.; Okabe, H.; Ohno, Y.; Nakai, N.; Ochiai, T.; Goto, K.; Ohira, Y.

2013-02-01

Neck muscles are known to play important roles in the maintenance of head posture against gravity. However, it is not known how the properties of neck muscle are influenced by gravity. Therefore, the current study was performed to investigate the responses of neck muscle (rhomboideus capitis) in mice to inhibition of gravity and/or increase to 2-G for 3 months to test the hypothesis that the properties of neck muscles are regulated in response to the level of mechanical load applied by the gravitational load. Three male wild type C57BL/10J mice (8 weeks old) were launched by space shuttle Discovery (STS-128) and housed in Japanese Experimental Module “KIBO” on the International Space Station in mouse drawer system (MDS) project, which was organized by Italian Space Agency. Only 1 mouse returned to the Earth alive after 3 months by space shuttle Atlantis (STS-129). Neck muscles were sampled from both sides within 3 hours after landing. Cage and laboratory control experiments were also performed on the ground. Further, 3-month ground-based control experiments were performed with 6 groups, i.e. pre-experiment, 3-month hindlimb suspension, 2-G exposure by using animal centrifuge, and vivarium control (n=5 each group). Five mice were allowed to recover from hindlimb suspension (including 5 cage control) for 3 months in the cage. Neck muscles were sampled bilaterally before and after 3-month suspension and 2-G exposure, and at the end of 3-month ambulation recovery. Spaceflight-associated shift of myosin heavy chain phenotype from type I to II and atrophy of type I fibers were observed. In response to spaceflight, 17 genes were up-regulated and 13 genes were down-regulated vs. those in the laboratory control. Expression of 6 genes were up-regulated and that of 88 genes were down-regulated by 3-month exposure to 2-G vs. the age-matched cage control. In response to chronic hindlimb suspension, 4 and 20 genes were up- or down-regulated. Further, 98 genes responded significantly to both hindlimb unloading and exposure to 2-G. Thirteen genes were up-regulated and 85 were down-regulated. In conclusion, long-term gravitational unloading of mouse caused shift of fiber phenotype toward fast-twitch type and atrophy of slow-twitch fibers in neck muscle. These responses were closely related to the up- or down-regulation of genes, suggesting that oxidative muscular metabolism may be inhibited in microgravity environment.

Inhibition of proliferation and induction of apoptosis in soft tissue sarcoma cells by interferon-α and retinoids

PubMed Central

Brodowicz, T; Wiltschke, C; Kandioler-Eckersberger, D; Grunt, T W; Rudas, M; Schneider, S M; Hejna, M; Budinsky, A; Zielinski, C C

1999-01-01

Uncontrolled proliferation and a defect of apoptosis constitute crucial elements in the development and progression of tumours. Among many other biological response modifiers known to influence these mechanisms, the efficacy of retinoids and interferons in the treatment of various malignant entities is currently matter of discussion. In the present study, we have investigated the effects of 9-cis-retinoic acid (9cRA), 13-cis-retinoic acid (13cRA), all-trans-retinoic acid (tRA) and interferon-α on proliferation and apoptosis of human soft tissue sarcoma (STS) cell lines HTB-82 (rhabdomyosarcoma), HTB-91 (fibrosarcoma), HTB-92 (liposarcoma), HTB-93 (synovial sarcoma) and HTB-94 (chondrosarcoma) in relation to p53 genotype as well as p53 expression. HTB-91, HTB-92 and HTB-94 STS cells exhibited mutant p53, whereas wild-type p53 was found in HTB-93 STS cells, and a normal p53 status in HTB-82 STS cells, carrying a silent point mutation only. Interferon-α, irrespective of p53 status, inhibited the proliferation of all five cell lines dose- and time-dependently. Similarly, 9cRA, 13cRA and tRA decreased the proliferation of HTB-82 and HTB-93 STS cells, whereas the proliferation of p53-mutated HTB-91, HTB-92 and HTB-94 STS cells remained unchanged. Furthermore, only 9cRA and tRA were capable of inducing apoptosis in HTB-82 and HTB-93 STS cells, whereas HTB-91, HTB-92 and HTB-94 STS cells did not undergo apoptosis under the influence of 9cRA or tRA. Retinoic acid receptor (RAR)-α and RAR-β mRNA were not detectable by Northern blot analysis in the five STS cell lines, whereas mRNA for the universal retinoic acid receptor, RAR-γ, was expressed in all STS cell lines indicating that retinoid resistance was not associated with a lack of RAR expression. Apoptosis was not induced by interferon-α or 13cRA in any of the five STS cell lines tested. Our results indicate that within the panel of tested STS cell lines, inhibition of proliferation and induction of apoptosis result from different mechanisms which differ in their dependence upon the presence of intact p53. © 1999 Cancer Research Campaign PMID:10424735

A sequence variant in human KALRN impairs protein function and coincides with reduced cortical thickness

PubMed Central

Russell, Theron A.; Blizinsky, Katherine D.; Cobia, Derin J.; Cahill, Michael; Xie, Zhong; Sweet, Robert A.; Duan, Jubao; Gejman, Pablo V.; Wang, Lei; Csernansky, John G.; Penzes, Peter

2014-01-01

Dendritic spine pathology is a key feature of several neuropsychiatric disorders. The Rac1 guanine nucleotide exchange factor kalirin-7 is critical for spine morphogenesis on cortical pyramidal neurons. Here we identify a rare coding variant in the KALRN gene region that encodes the catalytic domain, in a schizophrenia patient and his sibling with major depressive disorder. The D1338N substitution significantly diminished the protein's ability catalyze the activation of Rac1. Contrary to wild-type kalirin-7, kalirin-7-D1338N failed to increase spine size and density. Both subjects carrying the polymorphism displayed reduced cortical volume in the superior temporal sulcus (STS), a region implicated in schizophrenia. Consistent with this, mice with reduced kalirin expression showed reduced neuropil volume in the rodent homolog of the STS. These data suggest that single amino acid changes in proteins involved in dendritic spine function can have significant effects on the structure and function of the cerebral cortex. PMID:25224588

A sequence variant in human KALRN impairs protein function and coincides with reduced cortical thickness.

PubMed

Russell, Theron A; Blizinsky, Katherine D; Cobia, Derin J; Cahill, Michael E; Xie, Zhong; Sweet, Robert A; Duan, Jubao; Gejman, Pablo V; Wang, Lei; Csernansky, John G; Penzes, Peter

2014-09-16

Dendritic spine pathology is a key feature of several neuropsychiatric disorders. The Rac1 guanine nucleotide exchange factor kalirin-7 is critical for spine morphogenesis on cortical pyramidal neurons. Here we identify a rare coding variant in the KALRN gene region that encodes the catalytic domain, in a schizophrenia patient and his sibling with major depressive disorder. The D1338N substitution significantly diminished the protein's ability to catalyse the activation of Rac1. Contrary to wild-type kalirin-7, kalirin-7-D1338N failed to increase spine size and density. Both subjects carrying the polymorphism displayed reduced cortical volume in the superior temporal sulcus (STS), a region implicated in schizophrenia. Consistent with this, mice with reduced kalirin expression showed reduced neuropil volume in the rodent homologue of the STS. These data suggest that single amino acid changes in proteins involved in dendritic spine function can have significant effects on the structure and function of the cerebral cortex.

Mapping the new molecular landscape: social dimensions of epigenetics

PubMed Central

Pickersgill, Martyn; Niewöhner, Jörg; Müller, Ruth; Martin, Paul; Cunningham-Burley, Sarah

2013-01-01

Epigenetics is the study of changes in gene expression caused by mechanisms other than changes in the DNA itself. The field is rapidly growing and being widely promoted, attracting attention in diverse arenas. These include those of the social sciences, where some researchers have been encouraged by the resonance between imaginaries of development within epigenetics and social theory. Yet, sustained attention from science and technology studies (STS) scholars to epigenetics and the praxis it propels has been lacking. In this article, we reflexively consider some of the ways in which epigenetics is being constructed as an area of biomedical novelty and discuss the content and logics underlying the ambivalent promises being made by scientists working in this area. We then reflect on the scope, limits and future of engagements between epigenetics and the social sciences. Our discussion is situated within wider literatures on biomedicine and society, the politics of “interventionist STS,” and on the problems of “caseness” within empirical social science. PMID:24482610

Action understanding in the superior temporal sulcus region.

PubMed

Wyk, Brent C Vander; Hudac, Caitlin M; Carter, Elizabeth J; Sobel, David M; Pelphrey, Kevin A

2009-06-01

The posterior superior temporal sulcus (STS) region plays an important role in the perception of social acts, although its full role has not been completely clarified. This functional magnetic resonance imaging experiment examined activity in the STS region as participants viewed actions that were congruent or incongruent with intentions established by a previous emotional context. Participants viewed an actress express either a positive or a negative emotion toward one of two objects and then subsequently pick up one of them. If the object that was picked up had received positive regard, or if the object that was not picked up had received negative regard, the action was congruent; otherwise, the action was incongruent. Activity in the right posterior STS region was sensitive to the congruency between the action and the actress's emotional expression (i.e., STS activity was greater on incongruent than on congruent trials). These findings suggest that the posterior STS represents not only biological motion, but also how another person's motion is related to his or her intentions.

Deconstructing sub-clinical psychosis into latent-state and trait variables over a 30-year time span.

PubMed

Rössler, Wulf; Hengartner, Michael P; Ajdacic-Gross, Vladeta; Haker, Helene; Angst, Jules

2013-10-01

Our aim was to deconstruct the variance underlying the expression of sub-clinical psychosis symptoms into portions associated with latent time-dependent states and time-invariant traits. We analyzed data of 335 subjects from the general population of Zurich, Switzerland, who had been repeatedly measured between 1979 (age 20/21) and 2008 (age 49/50). We applied two measures of sub-clinical psychosis derived from the SCL-90-R, namely schizotypal signs (STS) and schizophrenia nuclear symptoms (SNS). Variance was decomposed with latent state-trait analysis and associations with covariates were examined with generalized linear models. At ages 19/20 and 49/50, the latent states underlying STS accounted for 48% and 51% of variance, whereas for SNS those estimates were 62% and 50%. Between those age classes, however, expression of sub-clinical psychosis was strongly associated with stable traits (75% and 89% of total variance in STS and SNS, respectively, at age 27/28). Latent states underlying variance in STS and SNS were particularly related to partnership problems over almost the entire observation period. STS was additionally related to employment problems, whereas drug-use was a strong predictor of states underlying both syndromes at age 19/20. The latent trait underlying expression of STS and SNS was particularly related to low sense of mastery and self-esteem and to high depressiveness. Although most psychosis symptoms are transient and episodic in nature, the variability in their expression is predominantly caused by stable traits. Those time-invariant and rather consistent effects are particularly influential around age 30, whereas the occasion-specific states appear to be particularly influential at ages 20 and 50. © 2013.

Prohibitin (PHB) inhibits apoptosis in rat granulosa cells (GCs) through the extracellular signal-regulated kinase 1/2 (ERK1/2) and the Bcl family of proteins.

PubMed

Chowdhury, Indrajit; Thompson, Winston E; Welch, Crystal; Thomas, Kelwyn; Matthews, Roland

2013-12-01

Mammalian ovarian follicular development is tightly regulated by crosstalk between cell death and survival signals, which include both endocrine and intra-ovarian regulators. Whether the follicle ultimately ovulates or undergoes atresia is dependent on the expression and actions of factors promoting follicular cell proliferation, differentiation or apoptosis. Prohibitin (PHB) is a highly conserved, ubiquitous protein that is abundantly expressed in granulosa cells (GCs) and associated with GC differentiation and apoptosis. The current study was designed to characterize the regulation of anti-apoptotic and pro-apoptotic factors in undifferentiated rat GCs (gonadotropin independent phase) governed by PHB. Microarray technology was initially employed to identify potential apoptosis-related genes, whose expression levels within GCs were altered by either staurosporine (STS) alone or STS in presence of ectopically over-expressed PHB. Next, immunoblot studies were performed to examine the expression patterns of selective Bcl-2 family members identified by the microarray analysis, which are commonly regulated in the intrinsic-apoptotic pathway. These studies were designed to measure protein levels of Bcl2 family in relation to expression of the acidic isoform (phosphorylated) PHB and the components of MEK-Erk1/2 pathway. These studies indicated that over-expression of PHB in undifferentiated GCs inhibit apoptosis which concomitantly results in an increased level of the anti-apoptotic proteins Bcl2 and Bclxl, reduced release of cytochrome c from mitochondria and inhibition of caspase-3 activity. In contrast, silencing of PHB expression resulted in change of mitochondrial morphology from the regular reticular network to a fragmented form, which enhanced sensitization of these GCs to the induction of apoptosis. Collectively, these studies have provided new insights on the PHB-mediated anti-apoptotic mechanism, which occurs in undifferentiated GCs through a PHB → Mek-Erk1/2 → Bcl/Bcl-xL pathway and may have important clinical implications.

STS-119 crew visit

NASA Image and Video Library

2009-05-05

Stennis Space Center Director Gene Goldman (r to l) presents a commemorative photo of a space shuttle main engine test firing to STS-119 Mission Commander Lee Archambault, Pilot Tony Antonelli and Mission Specialists Steve Swanson and Richard Arnold during the crew's May 5 visit to the facility.

KENNEDY SPACE CENTER, FLA. - In the Orbiter Processing Facility, KSC employee Gene Peavler works in the wheel area on the orbiter Discovery. The vehicle has undergone Orbiter Major Modifications in the past year. Discovery is scheduled to fly on mission STS-121 to the International Space Station.

NASA Image and Video Library

2003-12-09

KENNEDY SPACE CENTER, FLA. - In the Orbiter Processing Facility, KSC employee Gene Peavler works in the wheel area on the orbiter Discovery. The vehicle has undergone Orbiter Major Modifications in the past year. Discovery is scheduled to fly on mission STS-121 to the International Space Station.

LOH- RadGene experiment at Cell Biology Experiment Facility (CBEF)

NASA Image and Video Library

2009-02-20

ISS018-E-034074 (20 Feb. 2009) --- Astronaut Sandra Magnus, Expedition 18 flight engineer, works with the LOH- RadGene experiment near the Cell Biology Experiment Facility (CBEF) in the Kibo laboratory of the International Space Station. This experiment investigates alterations in immature immune cells that have been exposed to cosmic radiation. The samples were placed in culture bags and launched to the ISS on the STS-126 mission. After the experiment, frozen samples will be returned to the ground on the STS-119 mission.

EGFR expression in circulating tumor cells from high-grade metastatic soft tissue sarcomas.

PubMed

Braun, Alexcia Camila; de Mello, Celso Abdon Lopes; Corassa, Marcelo; Abdallah, Emne Ali; Urvanegia, Ana Cláudia; Alves, Vanessa Silva; Flores, Bianca C T C P; Díaz, Mônica; Nicolau, Ulisses Ribaldo; Silva, Virgilio Souza E; Calsavara, Vinicius; Paterlini-Brechót, Patrizia; Chinen, Ludmilla Thomé Domingos

2018-06-03

Soft tissue Sarcomas (STS) are rare malignances, with high mortality rates. Half of patients develop metastasis. The presence of isolated Circulating Tumor Cells (CTCs) and Circulating Tumor Microemboli (CTM) in the blood may be early markers of tumor invasion. Epidermal Growth Factor (EGF) family receptors can also influence this process. to quantify CTCs and identify CTM as well as the EGF Receptor (EGFR) protein expression in these cells and correlate with clinical outcome in metastatic STS. Approximately 8mL of blood was prospectively collected from patients with different types of high-grade STS, before the beginning of chemotherapy. The samples were processed and filtered by ISET (Rarecells, France) for the isolation and quantification of CTCs and CTMs. EGFR expression was analyzed by immunocytochemistry (ICC) on CTCs/ CTMs. We analyzed 18 patients with median age of 49 years (18-77 y). The positivity for EGFR protein expression in CTCs was observed in 93.75% of the patients. This result shows that targeting EGFR positive CTCs from STS origen can be translated in clinical benefit for some patients. In addition, if target therapy is chosen, the EGFR expression in CTCs can be used in follow-up to measure treatment effectiveness. This is the first study to demonstrate the expression of EGFR protein in CTCs from sarcoma patients. It may open an area for future investigations. The next step is to characterize CTCs in a larger cohort of patients to better understand the role of EGFR in sustaining tumor metastasis in sarcomas.

A Comprehensive Epidemiological Research for Clinical Vibrio parahaemolyticus in Shanghai

PubMed Central

Li, Huan; Tang, Rong; Lou, Yang; Cui, Zelin; Chen, Wenjing; Hong, Qing; Zhang, Zhaohuan; Malakar, Pradeep K.; Pan, Yingjie; Zhao, Yong

2017-01-01

Vibrio parahaemolyticus is one of the most important pathogen for seafood-borne gastroenteritis in Shanghai and the rest of the world. A total of 42 V. parahaemolyticus strains were isolated from 1900 fecal specimens collected from patients in Shanghai hospital presenting from January 2014 to December 2015. All isolates were evaluated for potential virulence factors [tdh, trh, and type three secretion system (T3SS) genes], typed using multilocus sequence typing (MLST) and screened for antimicrobial resistance phenotype and genotype. And for the first time, the relationship between virulence, genetic diversity and antimicrobial resistance of these isolates were identified. The results showed that 37 isolates carried the tdh gene (88.1%) and only seven isolates were positive for the trh gene. The T3SS1 and T3SS2 genes were detected in all strains and only trh-positive isolates are also containing the T3SS2β genes. MLST analysis of the 42 Shanghai isolates identified 20 sequence types (STs) with 16 novel STs and that these clinical V. parahaemolyticus strains showed high degrees of genetic diversity. All isolates expressed high levels of resistance against Ampicillin (100.0%), Streptomycin (100.0%), Cephazolin (92.9%), Kanamycin (92.8%) and Amikacin (90.5%), and eight out of 38 resistance genes (SHV, tet(B), strA, qnrA, gryA, qnrB, sulI, sulII) were detected in at least two isolates. This study confirms that antimicrobial resistance of clinical V. parahaemolyticus isolates is greater than those of environmental isolates. Furthermore, no clear correlation between antimicrobial resistance and virulence or genetic diversity was found in this study. These results add to epidemiological data of clinical V. parahaemolyticus isolates in Shanghai and highlight the need for additional mechanistic studies, especially antimicrobial resistance, to reduce the burden of disease caused by this pathogen in China. PMID:28642752

The Presence of ALK Alterations and Clinical Relevance of Crizotinib Treatment in Pediatric Solid Tumors.

PubMed

Felkai, Luca; Bánusz, Rita; Kovalszky, Ilona; Sápi, Zoltán; Garami, Miklós; Papp, Gergő; Karászi, Katalin; Varga, Edit; Csóka, Monika

2017-10-28

Soft tissue sarcomas (STS) and neuroblastomas (NBL), are childhood malignancies still associated with poor prognoses despite the overall improvement in childhood tumor survival of the past decades. Anaplastic lymphoma kinase (ALK) inhibition is promising new strategy to improve the outcome of these pediatric tumors. Eighteen histologic samples of pediatric STS and 19 NBL patients were analyzed for ALK abnormalities using fluorescent in situ hybridization (FISH) with break-apart probes and immunohistochemistry (IHC). ALK alterations were presented in 20 of the 37 sections. The presence of ALK alteration in NBL samples were detected using IHC in 84,2% of all cases compared to 21,1% FISH positivity. In STS cases the results were less different (IHC 16,7% vs FISH 22,2%). The difference can be explained by the different type of molecular alterations. FISH method detected translocation and amplification, but not the point mutation of ALK gene. IHC confirmed the diagnosis by detecting the expression of ALK protein.After ALK positivity was proven, the effectiveness and safety of the crizotinib therapy was examined in 4 patients (1 alveolar rhabdomyosarcoma (RMA), 1 embryonal rhabdomyosarcoma (RME), 1 inflammatory myofibroblastic tumor (IMT), 1 NBL). We observed continuous remission of the IMT patient, all other cases the inhibitor treatment was not curative.Our findings underline the importance of screening the ALK status parallel with both IHC and FISH. Crizotinib treatment had a long-term effect in ALK positive IMT patients, however itwas only temporary efficient in relapsed, progressive STS and NBL.

Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

PubMed Central

Yin, Supeng; Chen, Ping; You, Bo; Zhang, Yulong; Jiang, Bei; Huang, Guangtao; Yang, Zichen; Chen, Yu; Chen, Jing; Yuan, Zhiqiang; Zhao, Yan; Li, Ming; Hu, Fuquan; Gong, Yali; Peng, Yizhi

2018-01-01

Pseudomonas aeruginosa is the leading cause of infection in burn patients. The increasing carbapenem resistance of P. aeruginosa has become a serious challenge to clinicians. The present study investigated the molecular typing and carbapenem resistance mechanisms of 196 P. aeruginosa isolates from the bloodstream and wound surface of patients in our burn center over a period of 6 years. By multilocus sequence typing (MLST), a total of 58 sequence types (STs) were identified. An outbreak of ST111, a type that poses a high international risk, occurred in 2014. The isolates from wound samples of patients without bacteremia were more diverse and more susceptible to antibiotics than strains collected from the bloodstream or the wound surface of patients with bacteremia. Importantly, a large proportion of the patients with multisite infection (46.51%) were simultaneously infected by different STs in the bloodstream and wound surface. Antimicrobial susceptibility testing of these isolates revealed high levels of resistance to carbapenems, with 35.71% susceptibility to imipenem and 32.14% to meropenem. To evaluate mechanisms associated with carbapenem resistance, experiments were conducted to determine the prevalence of carbapenemase genes, detect alterations of the oprD porin gene, and measure expression of the ampC β-lactamase gene and the mexB multidrug efflux gene. The main mechanism associated with carbapenem resistance was mutational inactivation of oprD (88.65%), accompanied by overexpression of ampC (68.09%). In some cases, oprD was inactivated by insertion sequence element IS1411, which has not been found previously in P. aeruginosa. These findings may help control nosocomial P. aeruginosa infections and improve clinical practice. PMID:29896186

Macrophage Migration Inhibitory Factor and Stearoyl-CoA Desaturase 1: Potential Prognostic Markers for Soft Tissue Sarcomas Based on Bioinformatics Analyses

PubMed Central

Takahashi, Hiro; Nakayama, Robert; Hayashi, Shuhei; Nemoto, Takeshi; Murase, Yasuyuki; Nomura, Koji; Takahashi, Teruyoshi; Kubo, Kenji; Marui, Shigetaka; Yasuhara, Koji; Nakamura, Tetsuro; Sueo, Takuya; Takahashi, Anna; Tsutsumiuchi, Kaname; Ohta, Tsutomu; Kawai, Akira; Sugita, Shintaro; Yamamoto, Shinjiro; Kobayashi, Takeshi; Honda, Hiroyuki; Yoshida, Teruhiko; Hasegawa, Tadashi

2013-01-01

The diagnosis and treatment of soft tissue sarcomas (STSs) has been particularly difficult, because STSs are a group of highly heterogeneous tumors in terms of histopathology, histological grade, and primary site. Recent advances in genome technologies have provided an excellent opportunity to determine the complete biological characteristics of neoplastic tissues, resulting in improved diagnosis, treatment selection, and investigation of therapeutic targets. We had previously developed a novel bioinformatics method for marker gene selection and applied this method to gene expression data from STS patients. This previous analysis revealed that the extracted gene combination of macrophage migration inhibitory factor (MIF) and stearoyl-CoA desaturase 1 (SCD1) is an effective diagnostic marker to discriminate between subtypes of STSs with highly different outcomes. In the present study, we hypothesize that the combination of MIF and SCD1 is also a prognostic marker for the overall outcome of STSs. To prove this hypothesis, we first analyzed microarray data from 88 STS patients and their outcomes. Our results show that the survival rates for MIF- and SCD1-positive groups were lower than those for negative groups, and the p values of the log-rank test are 0.0146 and 0.00606, respectively. In addition, survival rates are more significantly different (p = 0.000116) between groups that are double-positive and double-negative for MIF and SCD1. Furthermore, in vitro cell growth inhibition experiments by MIF and SCD1 inhibitors support the hypothesis. These results suggest that the gene set is useful as a prognostic marker associated with tumor progression. PMID:24167613

Changes in gene expression and signal transduction in microgravity

NASA Technical Reports Server (NTRS)

Hughes-Fulford, M.

2001-01-01

Studies from space flights over the past three decades have demonstrated that basic physiological changes occur in humans during space flight. These changes include cephalic fluid shifts, loss of fluid and electrolytes, loss of muscle mass, space motion sickness, anemia, reduced immune response, and loss of calcium and mineralized bone. The cause of most of these manifestations is not known and until recently, the general approach was to investigate general systemic changes, not basic cellular responses to microgravity. This laboratory has recently studied gene growth and activation of normal osteoblasts (MC3T3-El) during spaceflight. Osteoblast cells were grown on glass coverslips and loaded in the Biorack plunger boxes. The osteoblasts were launched in a serum deprived state, activated in microgravity and collected in microgravity. The osteoblasts were examined for changes in gene expression and signal transduction. Approximately one day after growth activation significant changes were observed in gene expression in 0-G flight samples. Immediate early growth genes/growth factors cox-2, c-myc, bcl2, TGF beta1, bFGF and PCNA showed a significant diminished mRNA induction in microgravity FCS activated cells when compared to ground and 1-G flight controls. Cox-1 was not detected in any of the samples. There were no significant differences in the expression of reference gene mRNA between the ground, 0-G and 1-G samples. The data suggest that quiescent osteoblasts are slower to enter the cell cycle in microgravity and that the lack of gravity itself may be a significant factor in bone loss in spaceflight. Preliminary data from our STS 76 flight experiment support our hypothesis that a basic biological response occurs at the tissue, cellular, and molecular level in 0-G. Here we examine ground-based and space flown data to help us understand the mechanism of bone loss in microgravity.

Hierarchical Encoding of Social Cues in Primate Inferior Temporal Cortex

PubMed Central

Morin, Elyse L.; Hadj-Bouziane, Fadila; Stokes, Mark; Ungerleider, Leslie G.; Bell, Andrew H.

2015-01-01

Faces convey information about identity and emotional state, both of which are important for our social interactions. Models of face processing propose that changeable versus invariant aspects of a face, specifically facial expression/gaze direction versus facial identity, are coded by distinct neural pathways and yet neurophysiological data supporting this separation are incomplete. We recorded activity from neurons along the inferior bank of the superior temporal sulcus (STS), while monkeys viewed images of conspecific faces and non-face control stimuli. Eight monkey identities were used, each presented with 3 different facial expressions (neutral, fear grin, and threat). All facial expressions were displayed with both a direct and averted gaze. In the posterior STS, we found that about one-quarter of face-responsive neurons are sensitive to social cues, the majority of which being sensitive to only one of these cues. In contrast, in anterior STS, not only did the proportion of neurons sensitive to social cues increase, but so too did the proportion of neurons sensitive to conjunctions of identity with either gaze direction or expression. These data support a convergence of signals related to faces as one moves anteriorly along the inferior bank of the STS, which forms a fundamental part of the face-processing network. PMID:24836688

STS-124 crew visits Stennis

NASA Image and Video Library

2008-07-23

NASA's John C. Stennis Space Center Deputy Director Gene Goldman (center) welcomed members of the STS-124 Discovery space shuttle crew during their July 23 visit to the center. Crew members who visited Stennis were (l to r) Pilot Ken Ham, Mission Specialist Karen Nyberg, Kelly, and Mission Specialists Ron Garan and Mike Fossum.

A Bioinformatics Facility for NASA

NASA Technical Reports Server (NTRS)

Schweighofer, Karl; Pohorille, Andrew

2006-01-01

Building on an existing prototype, we have fielded a facility with bioinformatics technologies that will help NASA meet its unique requirements for biological research. This facility consists of a cluster of computers capable of performing computationally intensive tasks, software tools, databases and knowledge management systems. Novel computational technologies for analyzing and integrating new biological data and already existing knowledge have been developed. With continued development and support, the facility will fulfill strategic NASA s bioinformatics needs in astrobiology and space exploration. . As a demonstration of these capabilities, we will present a detailed analysis of how spaceflight factors impact gene expression in the liver and kidney for mice flown aboard shuttle flight STS-108. We have found that many genes involved in signal transduction, cell cycle, and development respond to changes in microgravity, but that most metabolic pathways appear unchanged.

Genetic analysis of an Escherichia coli syndrome.

PubMed

Lennette, E T; Apirion, D

1971-12-01

A mutant strain of Escherichia coli that fails to recover from prolonged (72 hr) starvation also fails to grow at 43 C. Extracts of this mutant strain show an increased ribonuclease II activity as compared to extracts of the parental strain, and stable ribonucleic acid is degraded to a larger extent in this strain during starvation. Ts(+) transductants and revertants were tested for all the above-mentioned phenotypes. All the Ts(+) transductants and revertants tested behaved like the Ts(+) parental strain, which suggests that all the observed phenotypes are caused by a single sts (starvation-temperature sensitivity) mutation. The reversion rate from sts(-) to sts(+) is rather low but is within the range of reversion rates for other single-site mutations. Three-point transduction crosses located this sts mutation between the ilv and rbs genes. The properties of sts(+)/sts(-) merozygotes suggested that the Ts(-) phenotype of this mutation is recessive.

Involvement of Right STS in Audio-Visual Integration for Affective Speech Demonstrated Using MEG

PubMed Central

Hagan, Cindy C.; Woods, Will; Johnson, Sam; Green, Gary G. R.; Young, Andrew W.

2013-01-01

Speech and emotion perception are dynamic processes in which it may be optimal to integrate synchronous signals emitted from different sources. Studies of audio-visual (AV) perception of neutrally expressed speech demonstrate supra-additive (i.e., where AV>[unimodal auditory+unimodal visual]) responses in left STS to crossmodal speech stimuli. However, emotions are often conveyed simultaneously with speech; through the voice in the form of speech prosody and through the face in the form of facial expression. Previous studies of AV nonverbal emotion integration showed a role for right (rather than left) STS. The current study therefore examined whether the integration of facial and prosodic signals of emotional speech is associated with supra-additive responses in left (cf. results for speech integration) or right (due to emotional content) STS. As emotional displays are sometimes difficult to interpret, we also examined whether supra-additive responses were affected by emotional incongruence (i.e., ambiguity). Using magnetoencephalography, we continuously recorded eighteen participants as they viewed and heard AV congruent emotional and AV incongruent emotional speech stimuli. Significant supra-additive responses were observed in right STS within the first 250 ms for emotionally incongruent and emotionally congruent AV speech stimuli, which further underscores the role of right STS in processing crossmodal emotive signals. PMID:23950977

Involvement of right STS in audio-visual integration for affective speech demonstrated using MEG.

PubMed

Hagan, Cindy C; Woods, Will; Johnson, Sam; Green, Gary G R; Young, Andrew W

2013-01-01

Speech and emotion perception are dynamic processes in which it may be optimal to integrate synchronous signals emitted from different sources. Studies of audio-visual (AV) perception of neutrally expressed speech demonstrate supra-additive (i.e., where AV>[unimodal auditory+unimodal visual]) responses in left STS to crossmodal speech stimuli. However, emotions are often conveyed simultaneously with speech; through the voice in the form of speech prosody and through the face in the form of facial expression. Previous studies of AV nonverbal emotion integration showed a role for right (rather than left) STS. The current study therefore examined whether the integration of facial and prosodic signals of emotional speech is associated with supra-additive responses in left (cf. results for speech integration) or right (due to emotional content) STS. As emotional displays are sometimes difficult to interpret, we also examined whether supra-additive responses were affected by emotional incongruence (i.e., ambiguity). Using magnetoencephalography, we continuously recorded eighteen participants as they viewed and heard AV congruent emotional and AV incongruent emotional speech stimuli. Significant supra-additive responses were observed in right STS within the first 250 ms for emotionally incongruent and emotionally congruent AV speech stimuli, which further underscores the role of right STS in processing crossmodal emotive signals.

LOH- RadGene experiment at Cell Biology Experiment Facility (CBEF)

NASA Image and Video Library

2009-02-20

ISS018-E-034555 (20 Feb. 2009) --- Astronaut Sandra Magnus, Expedition 18 flight engineer, takes a moment for a photo while working with the LOH- RadGene experiment at the Cell Biology Experiment Facility (CBEF) in the Kibo laboratory of the International Space Station. This experiment investigates genetic alterations in immature immune cells that have been exposed to cosmic radiation. The samples were placed in culture bags and launched to the ISS on the STS-126 mission. After the experiment, frozen samples will be returned to the ground on the STS-119 mission.

Spaceflight effects on T lymphocyte distribution, function and gene expression

PubMed Central

Gridley, Daila S.; Slater, James M.; Luo-Owen, Xian; Rizvi, Asma; Chapes, Stephen K.; Stodieck, Louis S.; Ferguson, Virginia L.; Pecaut, Michael J.

2009-01-01

The immune system is highly sensitive to stressors present during spaceflight. The major emphasis of this study was on the T lymphocytes in C57BL/6NTac mice after return from a 13-day space shuttle mission (STS-118). Spleens and thymuses from flight animals (FLT) and ground controls similarly housed in animal enclosure modules (AEM) were evaluated within 3–6 h after landing. Phytohemagglutinin-induced splenocyte DNA synthesis was significantly reduced in FLT mice when based on both counts per minute and stimulation indexes (P < 0.05). Flow cytometry showed that CD3+ T and CD19+ B cell counts were low in spleens from the FLT group, whereas the number of NK1.1+ natural killer (NK) cells was increased (P < 0.01 for all three populations vs. AEM). The numerical changes resulted in a low percentage of T cells and high percentage of NK cells in FLT animals (P < 0.05). After activation of spleen cells with anti-CD3 monoclonal antibody, interleukin-2 (IL-2) was decreased, but IL-10, interferon-γ, and macrophage inflammatory protein-1α were increased in FLT mice (P < 0.05). Analysis of cancer-related genes in the thymus showed that the expression of 30 of 84 genes was significantly affected by flight (P < 0.05). Genes that differed from AEM controls by at least 1.5-fold were Birc5, Figf, Grb2, and Tert (upregulated) and Fos, Ifnb1, Itgb3, Mmp9, Myc, Pdgfb, S100a4, Thbs, and Tnf (downregulated). Collectively, the data show that T cell distribution, function, and gene expression are significantly modified shortly after return from the spaceflight environment. PMID:18988762

Prognostic impact of mRNA levels of osteopontin splice variants in soft tissue sarcoma patients.

PubMed

Hahnel, Antje; Wichmann, Henri; Greither, Thomas; Kappler, Matthias; Würl, Peter; Kotzsch, Matthias; Taubert, Helge; Vordermark, Dirk; Bache, Matthias

2012-04-02

It is well known that osteopontin (OPN) plays an important role in tumor progression and that a high OPN expression level in several tumor entities correlates with poor prognosis in cancer patients. However, little is known about the prognostic relevance of the OPN mRNA splice variants. We analyzed the mRNA expression levels of different OPN splice variants in tumor tissue of 124 soft tissue sarcoma (STS) patients. Quantitative real-time PCR (qRT-PCR) was used to analyze the mRNA expression level of three OPN splice variants (OPN-a, -b and -c). The multivariate Cox's proportional hazard regression model revealed that high mRNA expression levels of OPN splice variants are significantly associated with poor prognosis in STS patients (n = 124). Women (n = 68) with high mRNA expression levels of OPN-a and OPN-b have an especially elevated risk of tumor-related death (OPN-a: RR = 3.0, P = 0.01, CI = 1.3-6.8; OPN-b: RR = 3.4, P = 0.01, CI = 1.4-8.2). In particular, we found that high mRNA expression levels of OPN-b and OPN-c correlated with a high risk of tumor-related death in STS patients that received radiotherapy (n = 52; OPN-b: RR = 10.3, P < 0.01, CI = 2.0-53.7; OPN-c: RR = 11.4, P < 0.01, CI = 2.2-59.3). Our study shows that elevated mRNA expression levels of OPN splice variants are negative prognostic and predictive markers for STS patients. Further studies are needed to clarify the impact of the OPN splice variants on prognosis.

Characterization of the gene encoding pinin/DRS/memA and evidence for its potential tumor suppressor function.

PubMed

Shi, Y; Ouyang, P; Sugrue, S P

2000-01-13

Several cell adhesion-related proteins have been shown to act as tumor-suppressors (TS) in the neoplastic progression of epithelial-derived tumors. Pinin/DRS/memA was first identified in our laboratory and it was shown to be a cell adhesion-related molecule. Our previous study demonstrated that restoration of pinin expression in transformed cells not only positively influenced cellular adhesive properties but also reversed the transformed phenotype to more epithelial-like. Here, we show by FISH analysis that the gene locus for pinin is within 14q13. The alignment of the pinin gene with STS markers localized the gene to the previously identified TS locus D14S75-D14S288. Northern analyses revealed diminished pinin mRNA in renal cell carcinomas (RCC) and certain cancer cell lines. Immunohistochemical examination of tumor samples demonstrated absent or greatly reduced pinin in transitional cell carcinoma (TCC) and RCC tumors. TCC-derived J82 cells as well as EcR-293 cells transfected with full-length pinin cDNA demonstrated inhibition of anchorage-independent growth of cells in soft agar. Furthermore, methylation analyses revealed that aberrant methylation of pinin CpG islands was correlated with decreased/absent pinin expression in a subset of tumor tissues. These data lend significant support to the hypothesis that pinin/DRS/memA may act as a tumor suppressor in certain types of cancers.

Spatially generalizable representations of facial expressions: Decoding across partial face samples.

PubMed

Greening, Steven G; Mitchell, Derek G V; Smith, Fraser W

2018-04-01

A network of cortical and sub-cortical regions is known to be important in the processing of facial expression. However, to date no study has investigated whether representations of facial expressions present in this network permit generalization across independent samples of face information (e.g., eye region vs mouth region). We presented participants with partial face samples of five expression categories in a rapid event-related fMRI experiment. We reveal a network of face-sensitive regions that contain information about facial expression categories regardless of which part of the face is presented. We further reveal that the neural information present in a subset of these regions: dorsal prefrontal cortex (dPFC), superior temporal sulcus (STS), lateral occipital and ventral temporal cortex, and even early visual cortex, enables reliable generalization across independent visual inputs (faces depicting the 'eyes only' vs 'eyes removed'). Furthermore, classification performance was correlated to behavioral performance in STS and dPFC. Our results demonstrate that both higher (e.g., STS, dPFC) and lower level cortical regions contain information useful for facial expression decoding that go beyond the visual information presented, and implicate a key role for contextual mechanisms such as cortical feedback in facial expression perception under challenging conditions of visual occlusion. Copyright © 2017 Elsevier Ltd. All rights reserved.

Intracrine sex steroid synthesis and signaling in human epidermal keratinocytes and dermal fibroblasts.

PubMed

Pomari, Elena; Dalla Valle, Luisa; Pertile, Paolo; Colombo, Lorenzo; Thornton, M Julie

2015-02-01

Peripheral intracrine sex steroid synthesis from adrenal precursors dehydroepiandrosterone (DHEA) and DHEA-sulfate has evolved in humans. We sought to establish if there are differences in intracrine, paracrine, and endocrine regulation of sex steroids by primary cultures of human skin epidermal keratinocytes and dermal fibroblasts. Microarray analysis identified multifunctional genes modulated by steroids, quantitative RT-PCR (qRT-PCR) mRNA expression, enzymatic assay aromatase activity, scratch assay cell migration, immunocytochemistry α-smooth muscle actin (α-SMA), and collagen gel fibroblast contraction. All steroidogenic components were present, although only keratinocytes expressed the organic anion organic anion transporter protein (OATP) 2B1 transporter. Both expressed the G-protein-coupled estrogen receptor (GPER1). Steroids modulated multifunctional genes, up-regulating genes important in repair and aging [angiopoietin-like 4 (ANGPTL4), chemokine (C-X-C motif) ligand 1 (CXCL1), lamin B1 (LMNB1), and thioredoxin interacting protein (TXNIP)]. DHEA-sulfate (DHEA-S), DHEA, and 17β-estradiol stimulated keratinocyte and fibroblast migration at early (4 h) and late (24-48 h) time points, suggesting involvement of genomic and nongenomic signaling. Migration was blocked by aromatase and steroid sulfatase (STS) inhibitors confirming intracrine synthesis to estrogen. Testosterone had little effect, implying it is not an intermediate. Steroids stimulated fibroblast contraction but not α-SMA expression. Mechanical wounding reduced fibroblast aromatase activity but increased keratinocyte activity, amplifying the bioavailability of intracellular estrogen. Cultured fibroblasts and keratinocytes provide a biologically relevant model system to investigate the complex pathways of sex steroid intracrinology in human skin. © FASEB.

Effects of spaceflight on murine skeletal muscle gene expression

PubMed Central

Allen, David L.; Bandstra, Eric R.; Harrison, Brooke C.; Thorng, Seiha; Stodieck, Louis S.; Kostenuik, Paul J.; Morony, Sean; Lacey, David L.; Hammond, Timothy G.; Leinwand, Leslie L.; Argraves, W. Scott; Bateman, Ted A.; Barth, Jeremy L.

2009-01-01

Spaceflight results in a number of adaptations to skeletal muscle, including atrophy and shifts toward faster muscle fiber types. To identify changes in gene expression that may underlie these adaptations, we used both microarray expression analysis and real-time polymerase chain reaction to quantify shifts in mRNA levels in the gastrocnemius from mice flown on the 11-day, 19-h STS-108 shuttle flight and from normal gravity controls. Spaceflight data also were compared with the ground-based unloading model of hindlimb suspension, with one group of pure suspension and one of suspension followed by 3.5 h of reloading to mimic the time between landing and euthanization of the spaceflight mice. Analysis of microarray data revealed that 272 mRNAs were significantly altered by spaceflight, the majority of which displayed similar responses to hindlimb suspension, whereas reloading tended to counteract these responses. Several mRNAs altered by spaceflight were associated with muscle growth, including the phosphatidylinositol 3-kinase regulatory subunit p85α, insulin response substrate-1, the forkhead box O1 transcription factor, and MAFbx/atrogin1. Moreover, myostatin mRNA expression tended to increase, whereas mRNA levels of the myostatin inhibitor FSTL3 tended to decrease, in response to spaceflight. In addition, mRNA levels of the slow oxidative fiber-associated transcriptional coactivator peroxisome proliferator-associated receptor (PPAR)-γ coactivator-1α and the transcription factor PPAR-α were significantly decreased in spaceflight gastrocnemius. Finally, spaceflight resulted in a significant decrease in levels of the microRNA miR-206. Together these data demonstrate that spaceflight induces significant changes in mRNA expression of genes associated with muscle growth and fiber type. PMID:19074574

Hierarchical Encoding of Social Cues in Primate Inferior Temporal Cortex.

PubMed

Morin, Elyse L; Hadj-Bouziane, Fadila; Stokes, Mark; Ungerleider, Leslie G; Bell, Andrew H

2015-09-01

Faces convey information about identity and emotional state, both of which are important for our social interactions. Models of face processing propose that changeable versus invariant aspects of a face, specifically facial expression/gaze direction versus facial identity, are coded by distinct neural pathways and yet neurophysiological data supporting this separation are incomplete. We recorded activity from neurons along the inferior bank of the superior temporal sulcus (STS), while monkeys viewed images of conspecific faces and non-face control stimuli. Eight monkey identities were used, each presented with 3 different facial expressions (neutral, fear grin, and threat). All facial expressions were displayed with both a direct and averted gaze. In the posterior STS, we found that about one-quarter of face-responsive neurons are sensitive to social cues, the majority of which being sensitive to only one of these cues. In contrast, in anterior STS, not only did the proportion of neurons sensitive to social cues increase, but so too did the proportion of neurons sensitive to conjunctions of identity with either gaze direction or expression. These data support a convergence of signals related to faces as one moves anteriorly along the inferior bank of the STS, which forms a fundamental part of the face-processing network. Published by Oxford University Press 2014. This work is written by (a) US Government employee(s) and is in the public domain in the US.

Dynamics of processing invisible faces in the brain: automatic neural encoding of facial expression information.

PubMed

Jiang, Yi; Shannon, Robert W; Vizueta, Nathalie; Bernat, Edward M; Patrick, Christopher J; He, Sheng

2009-02-01

The fusiform face area (FFA) and the superior temporal sulcus (STS) are suggested to process facial identity and facial expression information respectively. We recently demonstrated a functional dissociation between the FFA and the STS as well as correlated sensitivity of the STS and the amygdala to facial expressions using an interocular suppression paradigm [Jiang, Y., He, S., 2006. Cortical responses to invisible faces: dissociating subsystems for facial-information processing. Curr. Biol. 16, 2023-2029.]. In the current event-related brain potential (ERP) study, we investigated the temporal dynamics of facial information processing. Observers viewed neutral, fearful, and scrambled face stimuli, either visibly or rendered invisible through interocular suppression. Relative to scrambled face stimuli, intact visible faces elicited larger positive P1 (110-130 ms) and larger negative N1 or N170 (160-180 ms) potentials at posterior occipital and bilateral occipito-temporal regions respectively, with the N170 amplitude significantly greater for fearful than neutral faces. Invisible intact faces generated a stronger signal than scrambled faces at 140-200 ms over posterior occipital areas whereas invisible fearful faces (compared to neutral and scrambled faces) elicited a significantly larger negative deflection starting at 220 ms along the STS. These results provide further evidence for cortical processing of facial information without awareness and elucidate the temporal sequence of automatic facial expression information extraction.

High Prevalence of ESBL-Producing Klebsiella pneumoniae Causing Community-Onset Infections in China

PubMed Central

Zhang, Jing; Zhou, Kai; Zheng, Beiwen; Zhao, Lina; Shen, Ping; Ji, Jinru; Wei, Zeqing; Li, Lanjuan; Zhou, Jianying; Xiao, Yonghong

2016-01-01

The aim of this work was to investigate the epidemiological and genetic characteristics of ESBL-producing Klebsiella pneumoniae (ESBL-Kp) causing community-onset infections. K. pneumoniae isolates were collected from 31 Chinese secondary hospitals between August 2010 and 2011. Genes encoding ESBL and AmpC beta-lactamases were detected by PCR. The isolates were assigned to sequence types (STs) using multi-locus sequence typing (MLST). Eleven ESBL-Kp strains were selected for whole-genome sequencing (WGS) for investigating the genetic environment and plasmids encoding ESBL genes. A total of 578 K. pneumoniae isolates were collected, and 184 (31.8%) carried ESBL genes. The prevalence of ESBL-Kp varied from different geographical areas of China (10.2–50.3%). The three most prevalent ESBL genes were blaCTX-M-14 (n = 74), blaCTX-M-15 (n = 60), and blaCTX-M-3 (n = 40). MLST assigned 127 CTX-M-14 and CTX-M-15 producers to 54 STs, and CC17 was the most prevalent population (12.6%). STs (23, 37, and 86) that were known frequently associated with hypervirulent K. pneumoniae (hvKP) account for 14.1% (18/127). Phylogenetic analysis by concatenating the seven loci of MLST revealed the existence of ESBL-producing K. quasipneumoniae (two strains) and K. varricola (one strain), which was further confirmed by WGS. This study highlights the challenge of community-onset infections caused by ESBL-Kp in China. The prevalence of STs frequently associating with hvKP should be of concern. Surveillance of ESBL-KP causing community-onset infections now appears imperative. PMID:27895637

Normal voice processing after posterior superior temporal sulcus lesion.

PubMed

Jiahui, Guo; Garrido, Lúcia; Liu, Ran R; Susilo, Tirta; Barton, Jason J S; Duchaine, Bradley

2017-10-01

The right posterior superior temporal sulcus (pSTS) shows a strong response to voices, but the cognitive processes generating this response are unclear. One possibility is that this activity reflects basic voice processing. However, several fMRI and magnetoencephalography findings suggest instead that pSTS serves as an integrative hub that combines voice and face information. Here we investigate whether right pSTS contributes to basic voice processing by testing Faith, a patient whose right pSTS was resected, with eight behavioral tasks assessing voice identity perception and recognition, voice sex perception, and voice expression perception. Faith performed normally on all the tasks. Her normal performance indicates right pSTS is not necessary for intact voice recognition and suggests that pSTS activations to voices reflect higher-level processes. Copyright © 2017 Elsevier Ltd. All rights reserved.

Comparative Genome Analyses of Streptococcus suis Isolates from Endocarditis Demonstrate Persistence of Dual Phenotypic Clones

PubMed Central

Tohya, Mari; Watanabe, Takayasu; Maruyama, Fumito; Arai, Sakura; Ota, Atsushi; Athey, Taryn B. T.; Fittipaldi, Nahuel; Nakagawa, Ichiro; Sekizaki, Tsutomu

2016-01-01

Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S. suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs), ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels) ranging from one to 104 bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated) bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes. PMID:27433935

Dissociable roles of internal feelings and face recognition ability in facial expression decoding.

PubMed

Zhang, Lin; Song, Yiying; Liu, Ling; Liu, Jia

2016-05-15

The problem of emotion recognition has been tackled by researchers in both affective computing and cognitive neuroscience. While affective computing relies on analyzing visual features from facial expressions, it has been proposed that humans recognize emotions by internally simulating the emotional states conveyed by others' expressions, in addition to perceptual analysis of facial features. Here we investigated whether and how our internal feelings contributed to the ability to decode facial expressions. In two independent large samples of participants, we observed that individuals who generally experienced richer internal feelings exhibited a higher ability to decode facial expressions, and the contribution of internal feelings was independent of face recognition ability. Further, using voxel-based morphometry, we found that the gray matter volume (GMV) of bilateral superior temporal sulcus (STS) and the right inferior parietal lobule was associated with facial expression decoding through the mediating effect of internal feelings, while the GMV of bilateral STS, precuneus, and the right central opercular cortex contributed to facial expression decoding through the mediating effect of face recognition ability. In addition, the clusters in bilateral STS involved in the two components were neighboring yet separate. Our results may provide clues about the mechanism by which internal feelings, in addition to face recognition ability, serve as an important instrument for humans in facial expression decoding. Copyright © 2016 Elsevier Inc. All rights reserved.

KSC-2011-5762

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- STS-135 Commander Chris Ferguson expresses his gratitude to the thousands of workers who have processed, launched and landed the space shuttles for more than three decades during an employee appreciation event. Space shuttle Atlantis' final return from space at 5:57 a.m. EDT secured the space shuttle fleet's place in history and brought a close to the STS-135 mission and America's Space Shuttle Program. STS-135 delivered spare parts, equipment and supplies to the International Space Station. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Frankie Martin

Is spaceflight-induced immune dysfunction linked to systemic changes in metabolism?

PubMed Central

Mao, Xiao Wen; Bellinger, Denise L.; Jonscher, Karen R.; Stodieck, Louis S.; Ferguson, Virginia L.; Bateman, Ted A.; Mohney, Robert P.; Gridley, Daila S.

2017-01-01

The Space Shuttle Atlantis launched on its final mission (STS-135) on July 8, 2011. After just under 13 days, the shuttle landed safely at Kennedy Space Center (KSC) for the last time. Female C57BL/6J mice flew as part of the Commercial Biomedical Testing Module-3 (CBTM-3) payload. Ground controls were maintained at the KSC facility. Subsets of these mice were made available to investigators as part of NASA’s Bio-specimen Sharing Program (BSP). Our group characterized cell phenotype distributions and phagocytic function in the spleen, catecholamine and corticosterone levels in the adrenal glands, and transcriptomics/metabolomics in the liver. Despite decreases in most splenic leukocyte subsets, there were increases in reactive oxygen species (ROS)-related activity. Although there were increases noted in corticosterone levels in both the adrenals and liver, there were no significant changes in catecholamine levels. Furthermore, functional analysis of gene expression and metabolomic profiles suggest that the functional changes are not due to oxidative or psychological stress. Despite changes in gene expression patterns indicative of increases in phagocytic activity (e.g. endocytosis and formation of peroxisomes), there was no corresponding increase in genes related to ROS metabolism. In contrast, there were increases in expression profiles related to fatty acid oxidation with decreases in glycolysis-related profiles. Given the clear link between immune function and metabolism in many ground-based diseases, we propose a similar link may be involved in spaceflight-induced decrements in immune and metabolic function. PMID:28542224

Early development of fern gametophytes in microgravity

NASA Technical Reports Server (NTRS)

Roux, Stanley J.; Chatterjee, Ani; Hillier, Sheila; Cannon, Tom

2003-01-01

Dormant spores of the fern Ceratopteris richardii were flown on Shuttle mission STS-93 to evaluate the effects of micro-g on their development and on their pattern of gene expression. Prior to flight the spores were sterilized and sown into one of two environments: (1) Microscope slides in a video-microscopy module; and (2) Petri dishes. All spores were then stored in darkness until use. Spore germination was initiated on orbit after exposure to light. For the spores on microscope slides, cell level changes were recorded through the clear spore coat of the spores by video microscopy. After their exposure to light, spores in petri dishes were frozen in orbit at four different time points during which on earth gravity fixes the polarity of their development. Spores were then stored frozen in Biological Research in Canister units until recovery on earth. The RNAs from these cells and from 1-g control cells were extracted and analyzed on earth after flight to assay changes in gene expression. Video microscopy results revealed that the germinated spores developed normally in microgravity, although the polarity of their development, which is guided by gravity on earth, was random in space. Differential Display-PCR analyses of RNA extracted from space-flown cells showed that there was about a 5% change in the pattern of gene expression between cells developing in micro-g compared to those developing on earth. c2002 Published by Elsevier Science Ltd on behalf of COSPAR.

Is spaceflight-induced immune dysfunction linked to systemic changes in metabolism?

PubMed

Pecaut, Michael J; Mao, Xiao Wen; Bellinger, Denise L; Jonscher, Karen R; Stodieck, Louis S; Ferguson, Virginia L; Bateman, Ted A; Mohney, Robert P; Gridley, Daila S

2017-01-01

The Space Shuttle Atlantis launched on its final mission (STS-135) on July 8, 2011. After just under 13 days, the shuttle landed safely at Kennedy Space Center (KSC) for the last time. Female C57BL/6J mice flew as part of the Commercial Biomedical Testing Module-3 (CBTM-3) payload. Ground controls were maintained at the KSC facility. Subsets of these mice were made available to investigators as part of NASA's Bio-specimen Sharing Program (BSP). Our group characterized cell phenotype distributions and phagocytic function in the spleen, catecholamine and corticosterone levels in the adrenal glands, and transcriptomics/metabolomics in the liver. Despite decreases in most splenic leukocyte subsets, there were increases in reactive oxygen species (ROS)-related activity. Although there were increases noted in corticosterone levels in both the adrenals and liver, there were no significant changes in catecholamine levels. Furthermore, functional analysis of gene expression and metabolomic profiles suggest that the functional changes are not due to oxidative or psychological stress. Despite changes in gene expression patterns indicative of increases in phagocytic activity (e.g. endocytosis and formation of peroxisomes), there was no corresponding increase in genes related to ROS metabolism. In contrast, there were increases in expression profiles related to fatty acid oxidation with decreases in glycolysis-related profiles. Given the clear link between immune function and metabolism in many ground-based diseases, we propose a similar link may be involved in spaceflight-induced decrements in immune and metabolic function.

Bursch and Ross by EXPRESS rack 4 in the U.S. Lab during STS-110, Expedition Four joint OPS

NASA Image and Video Library

2002-04-12

ISS004-E-10029 (12 April 2002) --- Astronauts Daniel W. Bursch (left), Expedition Four flight engineer, and Jerry L. Ross, STS-110 mission specialist, work in the Destiny laboratory on the International Space Station (ISS).

Mapping of stripe rust resistance gene in an Aegilops caudate introgression line in wheat and its genetic association with leaf rust resistance.

PubMed

Toor, Puneet Inder; Kaur, Satinder; Bansal, Mitaly; Yadav, Bharat; Chhuneja, Parveen

2016-12-01

A pair of stripe rust and leaf rust resistance genes was introgressed from Aegilops caudata, a nonprogenitor diploid species with the CC genome, to cultivated wheat. Inheritance and genetic mapping of stripe rust resistance gene in backcrossrecombinant inbred line (BC-RIL) population derived from the cross of a wheat-Ae. caudata introgression line (IL) T291- 2(pau16060) with wheat cv. PBW343 is reported here. Segregation of BC-RILs for stripe rust resistance depicted a single major gene conditioning adult plant resistance (APR) with stripe rust reaction varying from TR-20MS in resistant RILs signifying the presence of some minor genes as well. Genetic association with leaf rust resistance revealed that two genes are located at a recombination distance of 13%. IL T291-2 had earlier been reported to carry introgressions on wheat chromosomes 2D, 3D, 4D, 5D, 6D and 7D. Genetic mapping indicated the introgression of stripe rust resistance gene on wheat chromosome 5DS in the region carrying leaf rust resistance gene LrAc, but as an independent introgression. Simple sequence repeat (SSR) and sequence-tagged site (STS) markers designed from the survey sequence data of 5DS enriched the target region harbouring stripe and leaf rust resistance genes. Stripe rust resistance locus, temporarily designated as YrAc, mapped at the distal most end of 5DS linked with a group of four colocated SSRs and two resistance gene analogue (RGA)-STS markers at a distance of 5.3 cM. LrAc mapped at a distance of 9.0 cM from the YrAc and at 2.8 cM from RGA-STS marker Ta5DS_2737450, YrAc and LrAc appear to be the candidate genes for marker-assisted enrichment of the wheat gene pool for rust resistance.

Spaceflight Alters Bacterial Gene Expression and Virulence and Reveals Role for Global Regulator Hfq

NASA Technical Reports Server (NTRS)

Wilson, J. W.; Ott, C. M.; zuBentrup, K. Honer; Ramamurthy R.; Quick, L.; Porwollik, S.; Cheng, P.; McClellan, M.; Tsaprailis, G.; Radabaugh, T.;

Molecular Mechanisms of Circadian Regulation During Spaceflight

NASA Technical Reports Server (NTRS)

Zanello, Susana; Boyle, Richard

2011-01-01

Disruption of the regular environmental circadian cues in addition to stringent and demanding operational schedules are two main factors that undoubtedly impact sleep patterns and vigilant performance in the astronaut crews during spaceflight. Most research is focused on the behavioral aspects of the risk of circadian desynchronization, characterized by fatigue and health and performance decrement. A common countermeasure for circadian re-entrainment utilizes blue-green light to entrain the circadian clock and mitigate this risk. However, an effective countermeasure targeting the photoreceptor system requires that the basic circadian molecular machinery remains intact during spaceflight. The molecular clock consists of sets of proteins that perform different functions within the clock machinery: circadian oscillators (genes whose expression levels cycle during the day, keep the pass of cellular time and regulate downstream effector genes), the effector or output genes (those which impact the physiology of the tissue or organism), and the input genes (responsible for sensing the environmental cues that allow circadian entrainment). The main environmental cue is light. As opposed to the known photoreceptors (rods and cones), the non-visual light stimulus is received by a subset of the population of retinal ganglion cells called intrinsically photosensitive retinal ganglion cells (ipRGC) that express melanopsin (opsin 4 -Opn4-) as the photoreceptor. We hypothesize that spaceflight may affect ipRGC and melanopsin expression, which may be a contributing cause of circadian disruption during spaceflight. To answer this question, eyes from albino Balb/cJ mice aboard STS-133 were collected for histological analysis and gene expression profiling of the retina at 1 and 7 days after landing. Both vivarium and AEM (animal enclosure module) mice were used as ground controls. Opn4 expression was analyzed by real time RT/qPCR and retinal sections were stained for Opn4 immunofluorescence. Opn4 was decreased (abrogated in one case) in retinas that concurrently showed higher evidence of oxidative stress. We propose that oxidative stress can lead to a decrease in melanopsin expression, likely via ipRGC loss or impairment, and thus, it can be a contributing factor to circadian disruption during spaceflight. Countermeasures contemplating the use of light should therefore be complemented with melanopsin expression maintenance and/or reduction in oxidative stress.

STS-121 crew visits SSC

NASA Technical Reports Server (NTRS)

2006-01-01

Astronauts Steve Lindsey (left), Stephanie Wilson, Lisa Nowak and Piers Sellers meet with employees at NASA Stennis Space Center. The crewmembers on NASA's space shuttle mission STS-121, which launched July 4, 2006, thanked SSC's workers for their dedication and safe work history. `We feel blessed that you are a part of the NASA family,' Wilson said. All four expressed gratitude for the reliability of the space shuttle's main engines, which helped propel the STS-121 crew into orbit on their 13-day mission.

STS-121 crew visits SSC

NASA Image and Video Library

2006-09-25

Astronauts Steve Lindsey (left), Stephanie Wilson, Lisa Nowak and Piers Sellers meet with employees at NASA Stennis Space Center. The crewmembers on NASA's space shuttle mission STS-121, which launched July 4, 2006, thanked SSC's workers for their dedication and safe work history. `We feel blessed that you are a part of the NASA family,' Wilson said. All four expressed gratitude for the reliability of the space shuttle's main engines, which helped propel the STS-121 crew into orbit on their 13-day mission.

KSC-2011-5761

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- The STS-135 crew members and NASA Kennedy Space Center Director Bob Cabana express their gratitude to the thousands of workers who have processed, launched and landed the space shuttles for more than three decades during an employee appreciation event. Space shuttle Atlantis' final return from space at 5:57 a.m. EDT secured the space shuttle fleet's place in history and brought a close to the STS-135 mission and America's Space Shuttle Program. STS-135 delivered spare parts, equipment and supplies to the International Space Station. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-5765

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- STS-135 Mission Specialist Sandy Magnus expresses her gratitude to the thousands of workers who have processed, launched and landed the space shuttles for more than three decades during an employee appreciation event. On the right is Pilot Doug Hurley. Space shuttle Atlantis' final return from space at 5:57 a.m. EDT secured the space shuttle fleet's place in history and brought a close to the STS-135 mission and America's Space Shuttle Program. STS-135 delivered spare parts, equipment and supplies to the International Space Station. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-5764

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- STS-135 Mission Specialist Rex Walheim expresses his gratitude to the thousands of workers who have processed, launched and landed the space shuttles for more than three decades during an employee appreciation event. On the right is Pilot Doug Hurley. Space shuttle Atlantis' final return from space at 5:57 a.m. EDT secured the space shuttle fleet's place in history and brought a close to the STS-135 mission and America's Space Shuttle Program. STS-135 delivered spare parts, equipment and supplies to the International Space Station. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Frankie Martin

Investigation of the Virulence Factors and Molecular Characterization of the Clonal Relations of Multidrug-Resistant Acinetobacter baumannii Isolates.

PubMed

Ali, Hayssam M; Salem, Mohamed Z M; El-Shikh, Mohamed S; Megeed, Ahmed Abdel; Alogaibi, Yahya A; Talea, Ibrahim Ahmed

2017-01-01

Multidrug-resistant (MDR) Acinetobacter baumannii infections are a great public health concern and demand continuous surveillance and antibiotic stewardship. Virulence traits and the pathogenicity of Acinetobacter are less studied compared with the molecular epidemiological and antibiotic resistance profile of this organism. In our present study, we investigated the primary characteristics contributing to the virulence of MDR A. baumannii isolates and compared them with avirulent isolates. A total of 32 well-characterized MDR A. baumannii clinical isolates and 22 avirulent isolates from a healthy individual were subjected to multilocus sequence typing and polymerase chain reaction (PCR) for a variety of biofilm-associated genes. Additionally, a number of in vitro tests were performed to determine virulence properties. Isolates were found to relate to six sequence types (STs) in which the dominant sequence was ST557 in clinical isolates, followed by ST195 and ST208. However, ST557 and ST222 were absent in avirulent isolates. All STs belonged to clonal complex 2 and clonal lineage 2, which is considered to be a universal clone. PCR analysis showed that most clinical isolates were positive for biofilm-forming genes, such as csu and bap, and also carried pga and ompA genes, which were less common in avirulent isolates. Biofilm formation, phospholipase C production, hemolytic activity, and acinetobactin production occurred significantly more frequently in clinical isolates compared with avirulent isolates. Though A. baumannii clonal lineages showed common virulence traits, they differed in virulent phenotype expression. These findings further support previous studies indicating that A. baumannii is a versatile pathogen with an ability to acquire iron and survive in iron-limiting conditions, highlighting the acinetobactin-mediated iron acquisition mechanisms involved in the pathogenesis of A. baumannii infections.

Microgravity

NASA Image and Video Library

1997-01-12

1 mm histone octamer crystal grown on STS-81. A very dynamic structure which functions in many aspects of gene regulation from control of gene activity to the more subtle mechanisms of genetic imprinting. Principle Investigator is Dan Carter of New Century Pharmaceuticals.

Major globally disseminated clonal complexes of antimicrobial resistant enterococci associated with infections in cancer patients in Brazil.

PubMed

Santos, Barbara A; Oliveira, Jéssica S; Cardoso, Nayara T; Barbosa, André V; Superti, Silvana V; Teixeira, Lúcia M; Neves, Felipe P G

2017-11-01

Cancer and hematological malignancies constitute major comorbidities in enterococcal infections, but little is known about the characteristics of enterococci affecting cancer patients. The aim of this study was to characterize 132 enterococcal clinical isolates obtained from cancer patients attending a Cancer Reference Center in Brazil between April 2013 and March 2014. Susceptibility to 17 antimicrobial agents was assessed by disk diffusion method. Resistance and virulence genes were investigated by PCR. Multilocus sequence typing (MLST) was performed for selected Enterococcus faecalis and Enterococcus faecium isolates. The predominant species was E. faecalis (108 isolates), followed by E. faecium (18), Enterococcus gallinarum (3), Enterococcus avium (2) and Enterococcus durans (1). Multidrug-resistant (MDR) isolates made up 44.7%, but all isolates were susceptible to fosfomycin, linezolid and glycopeptides. The most prevalent genes associated with erythromycin- and tetracycline-non susceptible isolates were erm(B) (47/71; 66.2%) and tet(M) (24/68; 35.3%), respectively. High-level resistance (HLR) to gentamicin was found in 22 (16.7%) isolates and 13 (59.1%) of them carried the aac(6')-Ie-aph(2″)-Ia gene. HLR to streptomycin was detected in 34 (25.8%) isolates, of which 15 (44.1%) isolates had the ant(6')-Ia gene. The most common virulence genes were gelE (48.9%), esp (30.5%) and asa1 (29.8%). MLST performed for 26 E. faecalis isolates revealed 18 different sequence-types (STs), with seven corresponding to novel STs (625, 626, 627, 628, 629, 630, and 635). On the other hand, nine of 10 E. faecium isolates analyzed by MLST belonged to a single clonal complex, comprised of mostly ST412, which emerged worldwide after mid-2000s, but also two novel STs (963 and 964). We detected major globally disseminated E. faecalis and E. faecium clonal complexes along with novel closely related STs, indicating the fitness and continuous evolution of these hospital-adapted lineages. Copyright © 2017 Elsevier B.V. All rights reserved.

Growth in spaceflight hardware results in alterations to the transcriptome and proteome

NASA Astrophysics Data System (ADS)

Basu, Proma; Kruse, Colin P. S.; Luesse, Darron R.; Wyatt, Sarah E.

2017-11-01

The Biological Research in Canisters (BRIC) hardware has been used to house many biology experiments on both the Space Transport System (STS, commonly known as the space shuttle) and the International Space Station (ISS). However, microscopic examination of Arabidopsis seedlings by Johnson et al. (2015) indicated the hardware itself may affect cell morphology. The experiment herein was designed to assess the effects of the BRIC-Petri Dish Fixation Units (BRIC-PDFU) hardware on the transcriptome and proteome of Arabidopsis seedlings. To our knowledge, this is the first transcriptomic and proteomic comparison of Arabidopsis seedlings grown with and without hardware. Arabidopsis thaliana wild-type Columbia (Col-0) seeds were sterilized and bulk plated on forty-four 60 mm Petri plates, of which 22 were integrated into the BRIC-PDFU hardware and 22 were maintained in closed containers at Ohio University. Seedlings were grown for approximately 3 days, fixed with RNAlater® and stored at -80 °C prior to RNA and protein extraction, with proteins separated into membrane and soluble fractions prior to analysis. The RNAseq analysis identified 1651 differentially expressed genes; MS/MS analysis identified 598 soluble and 589 membrane proteins differentially abundant both at p < .05. Fold enrichment analysis of gene ontology terms related to differentially expressed transcripts and proteins highlighted a variety of stress responses. Some of these genes and proteins have been previously identified in spaceflight experiments, indicating that these genes and proteins may be perturbed by both conditions.

Towards cloning the WAS-gene locus: YAC-contigs and PFGE analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meindi, A.; Schindelhauer, D.; Hellebrand, H.

1994-09-01

Patients with X-linked recessive Wiskott-Aldrich syndrome (WAS) manifest eczema, thrombocytopenia and severe immunodeficiency. Mapping studies place the WAS gene locus between the markers TIMP and DXS255 which both have been shown to be recombinant with the disease locus. Linkage analysis in eight families including a large Swiss family showed tight linkage of the disease to the loci DXS255 and DXS1126 and exclusion of TIMP as well as polymorphic loci adjacent to the OATL1 pseudogene cluster (e.g., DXS6616). Physical mapping with established YAC contigs and a radiation hybrid encompassing the Xp11.22-11.3 region revealed the loci order TIMP-PFC-elk1-DXS1367-DXS6616-OATL1-(DXS11260DXS226)-C5-3-TGE-3, SYP and (DXS255-DXS146). Themore » markers TIMP and C5-3 are contained on the same 1.6 Mb MluI-fragment. A novel expressed sequence (R1) could be placed between elk-1 and the PFC gene while the STS C5-3 could be localized adjacent to DXS1126. The gene cluster around DXS1126 could be connected with the TFE-3 and synaptophysin genes which map on the same 400 kb MluI fragment and two overlapping YACs. The minimum distance between SYP and DXS255 is 1.2 Mb; the maximum distance is 2.2 Mb. Expressed sequences which are obtained from a cosmid contig around DXS1126 and C5-3 are being used for mutation screening in WAS patients.« less

The APP intracellular domain (AICD) potentiates ER stress-induced apoptosis.

PubMed

Kögel, Donat; Concannon, Caoimhín G; Müller, Thorsten; König, Hildegard; Bonner, Caroline; Poeschel, Simone; Chang, Steffi; Egensperger, Rupert; Prehn, Jochen H M

2012-09-01

Here we employed human SHEP neuroblastoma cells either stably or inducibly expressing the amyloid precursor protein (APP) intracellular domain (AICD) to investigate its ability to modulate stress-induced cell death. Analysis of effector caspase activation revealed that AICD overexpression was specifically associated with an increased sensitivity to apoptosis induced by the 2 endoplasmic reticulum (ER) stressors thapsigargin and tunicamycin, but not by staurosporine (STS). Basal and ER stress-induced expression of Bip/Grp78 and C/EBP-homologous protein/GADD153 were not altered by AICD implying that AICD potentiated cell death downstream or independent of the conserved unfolded protein response (UPR). Interestingly, quantitative polymerase chain reaction analysis and reporter gene assays revealed that AICD significantly downregulated messenger RNA levels of the Alzheimer's disease susceptibility gene ApoJ/clusterin, indicating transcriptional repression. Knockdown of ApoJ/clusterin mimicked the effect of AICD on ER stress-induced apoptosis, but had no discernible effect on staurosporine-induced cell death. Our data suggest that altered levels of AICD may abolish the prosurvival function of ApoJ/clusterin and increase the susceptibility of neurons to ER stress-mediated cell death, a pathway that may contribute to the pathogenesis of Alzheimer's disease. Copyright © 2012 Elsevier Inc. All rights reserved.

Altered skeletal pattern of gene expression in response to spaceflight and hindlimb elevation

NASA Technical Reports Server (NTRS)

Bikle, D. D.; Harris, J.; Halloran, B. P.; Morey-Holton, E.

1994-01-01

Spaceflight leads to osteopenia, in part by inhibiting bone formation. Using an animal model (hindlimb elevation) that simulates the weightlessness of spaceflight, we and others showed a reversible inhibition of bone formation and bone mineralization. In this study, we have measured the mRNA levels of insulin-like growth factor I (IGF-I), IGF-I receptor (IGF-IR), alkaline phosphatase, and osteocalcin in the tibiae of rats flown aboard National Aeronautics and Space Administration Shuttle Flight STS-54 and compared the results with those obtained from their ground-based controls and from the bones of hindlimb-elevated animals. Spaceflight and hindlimb elevation transiently increase the mRNA levels for IGF-I, IGF-IR, and alkaline phosphatase but decrease the mRNA levels for osteocalcin. The changes in osteocalcin and alkaline phosphatase mRNA levels are consistent with a shift toward decreased maturation, whereas the rise in IGF-I and IGF-IR mRNA levels may indicate a compensatory response to the fall in bone formation. We conclude that skeletal unloading during spaceflight or hindlimb elevation resets the pattern of gene expression in the osteoblast, giving it a less mature profile.

The effect of a microgravity (space) environment on the expression of expansins from the peg and root tissues of Cucumis sativus

NASA Technical Reports Server (NTRS)

Link, B. M.; Wagner, E. R.; Cosgrove, D. J.

2001-01-01

In young cucumber seedlings, the peg is a polar outgrowth of tissue that functions by snagging the seed coat, thereby freeing the cotyledons. The development of the peg is thought to be gravity-dependent and has become a model system for plant-gravity response. Peg development requires rapid cell expansion, a process thought to be catalyzed by alpha-expansins, and thus was a good system to identify expansins that were regulated by gravity. This study identified 7 new alpha-expansin cDNAs from cucumber seedlings (Cucumis sativus L. cv Burpee Hybrid II) and examined their expression patterns. Two alpha-expansins (CsExp3 and CsExp4) were more highly expressed in the peg and the root. Earlier reports stated that pegs tend not to form in the absence of gravity, so the expression levels were compared in the pegs of seedlings grown in space (STS-95), on a clinostat, and on earth (1 g). Pegs were observed to form at high frequency on clinostat and space-grown seedlings, yet on clinostats there was more than a 4-fold reduction in the expression of CsExp3 in the pegs of seedlings grown on clinostats vs. those grown at 1 g, while the CsExp4 gene appeared to be turned off (below detection limits). There were no detectable differences in expansin gene expression levels for the pegs of seedlings grown in space or in the orbiter environmental simulator (OES) (1 g) at NASA. The microgravity environment did not affect the expression of CsExp3 or CsExp4, and the clinostat did not simulate the microgravity environment well.

Genetic diversity analysis of Leuconostoc mesenteroides from Korean vegetables and food products by multilocus sequence typing.

PubMed

Sharma, Anshul; Kaur, Jasmine; Lee, Sulhee; Park, Young-Seo

2018-06-01

In the present study, 35 Leuconostoc mesenteroides strains isolated from vegetables and food products from South Korea were studied by multilocus sequence typing (MLST) of seven housekeeping genes (atpA, groEL, gyrB, pheS, pyrG, rpoA, and uvrC). The fragment sizes of the seven amplified housekeeping genes ranged in length from 366 to 1414 bp. Sequence analysis indicated 27 different sequence types (STs) with 25 of them being represented by a single strain indicating high genetic diversity, whereas the remaining 2 were characterized by five strains each. In total, 220 polymorphic nucleotide sites were detected among seven housekeeping genes. The phylogenetic analysis based on the STs of the seven loci indicated that the 35 strains belonged to two major groups, A (28 strains) and B (7 strains). Split decomposition analysis showed that intraspecies recombination played a role in generating diversity among strains. The minimum spanning tree showed that the evolution of the STs was not correlated with food source. This study signifies that the multilocus sequence typing is a valuable tool to access the genetic diversity among L. mesenteroides strains from South Korea and can be used further to monitor the evolutionary changes.

Identification of Haemophilus influenzae clones associated with invasive disease a decade after introduction of H. influenzae serotype b vaccination in Italy.

PubMed

Giufrè, Maria; Cardines, Rita; Accogli, Marisa; Pardini, Manuela; Cerquetti, Marina

2013-08-01

The introduction of Haemophilus influenzae serotype b (Hib) conjugate vaccines has changed the epidemiology of invasive H. influenzae disease, with a shift in the predominant serotype from Hib to nonencapsulated H. influenzae (ncHi). The objective of this study was to identify the genotypes/clones associated with invasive H. influenzae disease in Italy. Eighty-seven H. influenzae strains isolated in the years 2009 to 2011 within the National Surveillance of Invasive Bacterial Disease program were analyzed. Strains were characterized by serotyping, antimicrobial susceptibility testing, and multilocus sequence typing (MLST). Genetic polymorphisms in the bla(TEM) gene promoter region as well as the occurrence of both adhesin genes (hmwA and hia) and the IgA1 protease-encoding gene (igaB) were also investigated. Of 87 strains, 67 were ncHi and 20 were encapsulated. Eleven strains were β-lactamase positive, harboring the bla(TEM) gene. Most bla(TEM) genes (10/11) were associated with a Pdel promoter region exhibiting a 135-bp deletion; the remaining strain possessed the Pa/Pb overlapping promoter. MLST analysis showed that encapsulated isolates were clonal, with each serotype sharing a few related sequence types (STs). Forty-six different STs were identified among the 67 ncHi strains. Despite this heterogeneity, a group of closely related STs (ST103, ST139, and ST145) encompassed almost 25% of all ncHi strains and 45.5% of the β-lactamase producers carrying the Pdel promoter. These major ST clones were found to be associated with the hmwA gene but not with the igaB gene. To conclude, although the heterogeneity of the ncHi population was confirmed, diffusion of major successful ST clones was documented.

KSC-2009-6017

NASA Image and Video Library

2009-10-30

CAPE CANAVERAL, Fla. - At NASA's Kennedy Space Center in Florida, workers monitor the lift of the canister containing the payload for space shuttle Atlantis' STS-129 mission to the International Space Station - Express Logistics Carriers 1 and 2 - into the Payload Changeout Room at Launch Pad 39A. Next, the payload will be installed in Atlantis' payload bay. The STS-129 crew will deliver two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Launch is set for Nov. 16. For information on the STS-129 mission objectives and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Amanda Diller

Dynamic facial expressions evoke distinct activation in the face perception network: a connectivity analysis study.

PubMed

Foley, Elaine; Rippon, Gina; Thai, Ngoc Jade; Longe, Olivia; Senior, Carl

2012-02-01

Very little is known about the neural structures involved in the perception of realistic dynamic facial expressions. In the present study, a unique set of naturalistic dynamic facial emotional expressions was created. Through fMRI and connectivity analysis, a dynamic face perception network was identified, which is demonstrated to extend Haxby et al.'s [Haxby, J. V., Hoffman, E. A., & Gobbini, M. I. The distributed human neural system for face perception. Trends in Cognitive Science, 4, 223-233, 2000] distributed neural system for face perception. This network includes early visual regions, such as the inferior occipital gyrus, which is identified as insensitive to motion or affect but sensitive to the visual stimulus, the STS, identified as specifically sensitive to motion, and the amygdala, recruited to process affect. Measures of effective connectivity between these regions revealed that dynamic facial stimuli were associated with specific increases in connectivity between early visual regions, such as the inferior occipital gyrus and the STS, along with coupling between the STS and the amygdala, as well as the inferior frontal gyrus. These findings support the presence of a distributed network of cortical regions that mediate the perception of different dynamic facial expressions.

KSC-2011-5760

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- STS-135 Commander Chris Ferguson, left, and NASA Kennedy Space Center Director Bob Cabana express their gratitude to the thousands of workers who have processed, launched and landed the space shuttles for more than three decades during an employee appreciation event. Space shuttle Atlantis' final return from space at 5:57 a.m. EDT secured the space shuttle fleet's place in history and brought a close to the STS-135 mission and America's Space Shuttle Program. STS-135 delivered spare parts, equipment and supplies to the International Space Station. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-5763

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- STS-135 Pilot Doug Hurley expresses his gratitude to the thousands of workers who have processed, launched and landed the space shuttles for more than three decades during an employee appreciation event. On the left is Mission Specialist Rex Walheim and to the right is Commander Chris Ferguson. Space shuttle Atlantis' final return from space at 5:57 a.m. EDT secured the space shuttle fleet's place in history and brought a close to the STS-135 mission and America's Space Shuttle Program. STS-135 delivered spare parts, equipment and supplies to the International Space Station. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Frankie Martin

Potential for immunotherapy in soft tissue sarcoma

PubMed Central

Tseng, William W; Somaiah, Neeta; Engleman, Edgar G

2015-01-01

Soft tissue sarcomas (STS) are rare, heterogeneous tumors of mesenchymal origin. Despite optimal treatment, a large proportion of patients will develop recurrent and metastatic disease. For these patients, current treatment options are quite limited. Significant progress has been made recently in the use of immunotherapy for the treatment of other solid tumors (e.g. prostate cancer, melanoma). There is a strong rationale for immunotherapy in STS, based on an understanding of disease biology. For example, STS frequently have chromosomal translocations which result in unique fusion proteins and specific subtypes have been shown to express cancer testis antigens. In this review, we discuss the current status of immunotherapy in STS, including data from human studies with cancer vaccines, adoptive cell therapy, and immune checkpoint blockade. Further research into STS immunology is needed to help design logical, subtype-specific immunotherapeutic strategies. PMID:25625925

Sonication, Vacuum Infiltration and Thiol Compounds Enhance the Agrobacterium-Mediated Transformation Frequency of Withania somnifera (L.) Dunal

PubMed Central

Sivanandhan, Ganeshan; Kapil Dev, Gnajothi; Theboral, Jeevaraj; Selvaraj, Natesan; Ganapathi, Andy; Manickavasagam, Markandan

2015-01-01

In the present study, we have established a stable transformation protocol via Agrobacterium tumafacines for the pharmaceutically important Withania somnifera. Six day-old nodal explants were used for 3 day co-cultivation with Agrobacterium tumefaciens strain LBA4404 harbouring the vector pCAMIBA2301. Among the different injury treatments, sonication, vacuum infiltration and their combination treatments tested, a vacuum infiltration for 10 min followed by sonication for 10 sec with A. tumefaciens led to a higher transient GUS expression (84% explants expressing GUS at regenerating sites). In order to improve gene integration, thiol compounds were added to co-cultivation medium. A combined treatment of L-Cys at 100 mg/l, STS at 125 mg/l, DTT at 75 mg/l resulted in a higher GUS expression (90%) in the nodal explants. After 3 days of co-cultivation, the explants were subjected to three selection cycles with increasing concentrations of kanamycin [100 to 115 mg/l]. The integration and expression of gusA gene in T0 and T1 transgenic plants were confirmed by polymerase chain reaction (PCR), and Southern blott analysis. These transformed plants (T0 and T1) were fertile and morphologically normal. From the present investigation, we have achieved a higher transformation efficiency of (10%). Withanolides (withanolide A, withanolide B, withanone and withaferin A) contents of transformed plants (T0 and T1) were marginally higher than control plants. PMID:25927703

Microgravity

NASA Image and Video Library

1997-01-12

This is a large 2 mm crystal of histone octamer, grown on STS-81. A very dynamic structure which functions in many aspects of gene regulation from control of gene activity to the more subtle mechanisms of genetic imprinting. Principle Investigator is Dan Carter of New Century Pharmaceuticals.

[Sequence-based typing of enviromental Legionella pneumophila isolates in Guangzhou].

PubMed

Zhang, Ying; Qu, Pinghua; Zhang, Jian; Chen, Shouyi

2011-03-01

To characterize the genes of Legionella pneumophila isolated from different water source in Guangzhou from 2006 to 2009. To genotype the strains by using sequence-based typing (SBT) scheme. In total 44 L. pneumophila strains were identified by SBT with 7 diversifying genes of flaA, asd, mip, pilE, mompS, proA and neuA. Analysis of the amplicons sequence was taken in the European Working Group for Legionella Infections (EWGLI) international SBT database to obtain the allelic profiles and sequence types (STs). Serogroups were typed by latex agglutination test. Data from SBT revealed a high diversity among the strains and ST01 accounts for 30% (13/ 44). Fifteen new STs were discovered from 20 STs and 2 of them were newly assigned (ST887 and ST888) by EWGLI. SBT Phylogenetic tree was generated by SplitsTree and BURST programs. High diversity and specificity were observed of the L. pneumophila strains in Guangzhou. SBT is useful for L. pneumophila genomic study and epidemiological surveillance.

Fine mapping of powdery mildew resistance genes PmTb7A.1 and PmTb7A.2 in Triticum boeoticum (Boiss.) using the shotgun sequence assembly of chromosome 7AL.

PubMed

Chhuneja, Parveen; Yadav, Bharat; Stirnweis, Daniel; Hurni, Severine; Kaur, Satinder; Elkot, Ahmed Fawzy; Keller, Beat; Wicker, Thomas; Sehgal, Sunish; Gill, Bikram S; Singh, Kuldeep

2015-10-01

A novel powdery mildew resistance gene and a new allele of Pm1 were identified and fine mapped. DNA markers suitable for marker-assisted selection have been identified. Powdery mildew caused by Blumeria graminis is one of the most important foliar diseases of wheat and causes significant yield losses worldwide. Diploid A genome species are an important genetic resource for disease resistance genes. Two powdery mildew resistance genes, identified in Triticum boeoticum (A(b)A(b)) accession pau5088, PmTb7A.1 and PmTb7A.2 were mapped on chromosome 7AL. In the present study, shotgun sequence assembly data for chromosome 7AL were utilised for fine mapping of these Pm resistance genes. Forty SSR, 73 resistance gene analogue-based sequence-tagged sites (RGA-STS) and 36 single nucleotide polymorphism markers were designed for fine mapping of PmTb7A.1 and PmTb7A.2. Twenty-one RGA-STS, 8 SSR and 13 SNP markers were mapped to 7AL. RGA-STS markers Ta7AL-4556232 and 7AL-4426363 were linked to the PmTb7A.1 and PmTb7A.2, at a genetic distance of 0.6 and 6.0 cM, respectively. The present investigation established that PmTb7A.1 is a new powdery mildew resistance gene that confers resistance to a broad range of Bgt isolates, whereas PmTb7A.2 most probably is a new allele of Pm1 based on chromosomal location and screening with Bgt isolates showing differential reaction on lines with different Pm1 alleles. The markers identified to be linked to the two Pm resistance genes are robust and can be used for marker-assisted introgression of these genes to hexaploid wheat.

Digestive enzyme expression and epithelial structure of small intestine in neonatal rats after 16 days spaceflight

NASA Astrophysics Data System (ADS)

Miyake, M.; Yamasaki, M.; Hazama, A.; Ijiri, K.; Shimizu, T.

It is important to assure whether digestive system can develop normally in neonates during spaceflight. Because the small intestine changes its function and structure drastically around weaning known as redifferentiation. Lactase expression declines and sucrase increases in small intestine for digestion of solid food before weaning. In this paper, we compared this enzyme transition and structural development of small intestine in neonatal rats after spaceflight. To find digestive genes differentially expressed in fight rats, DNA membrane macroarray was also used. Eight-day old rats were loaded to Space Shuttle Columbia, and housed in the animal facility for 16 days in space (STS-90, Neurolab mission). Two control groups (AGC; asynchronous ground control and VIV; vivarium) against flight group (FLT) were prepared. There was no difference in structure (crypt depth) and cell differentiation of epithelium between FLT and AGC by immunohistochemical analysis. We found that the amount of sucrase mRNA compared to lactase was decreased in FLT by RT-PCR. It reflected the enzyme transition was inhibited. Increase of 5 genes (APO A-I, APO A-IV, ACE, aFABP and aminopeptidase M) and decrease of carboxypeptidase-D were detected in FLT using macroarray. We think nutrition differences (less nourishment and late weaning) during spaceflight may cause inhibition of enzyme transition at least partly. The weightlessness might contribute to the inhibition through behavioral change.

Full length view of the Spacelab module

NASA Image and Video Library

2016-08-12

STS083-312-031 (4-8 April 1997) --- Payload specialist Gregory T. Linteris (left) is seen at the Mid Deck Glove Box (MGBX), while astronaut Donald A. Thomas, mission specialist, works at the Expedite the Processing of Experiments to Space Station (EXPRESS) rack. MGBX is a facility that allows scientists the capability of doing tests on hardware and materials that are not approved to be handled in the open Spacelab. It is equipped with photographic, video and data recording capability, allowing a complete record of experiment operations. Experiments performed on STS-83 were Bubble Drop Nonlinear Dynamics and Fiber Supported Droplet Combustion. EXPRESS is designed to provide accommodations for Sub-rack payloads on Space Station. For STS-83, it held two payloads. The Physics of Hard Colloidal Spheres (PHaSE) and ASTRO-Plant Generic Bioprocessing Apparatus (ASTRO-PGBA), a facility with light and atmospheric controls which supports plant growth for commercial research.

Photographic documentation of the STS-107 Memorial at the JSC Mall

NASA Image and Video Library

2003-02-04

JSC2003-E-05938 (4 February 2003) --- President George W. Bush addresses the crowd on the mall of the Johnson Space Center during the memorial for the Columbia astronauts. Seated from the left are Captain Gene Theriot, Chaplain Corps (USN); NASA Administrator Sean O’Keefe; and astronaut Kent V. Rominger, Chief of the Astronaut Office. A portrait of the STS-107 Columbia crew is visible at left.

KSC-2011-5766

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- The STS-135 crew members express their gratitude to the thousands of workers who have processed, launched and landed the space shuttles for more than three decades during an employee appreciation event. From left, are Mission Specialists Rex Walheim and Sandy Magnus, Commander Chris Ferguson, and Pilot Doug Hurley. Space shuttle Atlantis' final return from space at 5:57 a.m. EDT secured the space shuttle fleet's place in history and brought a close to the STS-135 mission and America's Space Shuttle Program. STS-135 delivered spare parts, equipment and supplies to the International Space Station. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Frankie Martin

Systemic Microgravity Response: Utilizing GeneLab to Develop Hypotheses for Spaceflight Risks

NASA Technical Reports Server (NTRS)

Beheshti, Afshin; Ray, Shayoni; Fogle, Homer W.; Berrios, Daniel C.; Costes, Sylvain V.

2017-01-01

Biological risks associated with microgravity are a major concern for long-term space travel. Although determination of risk has been a focus for NASA research, data examining systemic (i.e., multi- or pan-tissue) responses to space flight are sparse. To perform our analysis, we utilized the NASA GeneLab database which is a publicly available repository containing a wide array of omics results from experiments conducted with: i) with different flight conditions (space shuttle (STS) missions vs. International Space Station (ISS); ii) a variety of tissues; and 3) assays that measure epigenetic, transcriptional, and protein expression changes. Meta-analysis of the transcriptomic data from 7 different murine and rat data sets, examining tissues such as liver, kidney, adrenal gland, thymus, mammary gland, skin, and skeletal muscle (soleus, extensor digitorum longus, tibialis anterior, quadriceps, and gastrocnemius) revealed for the first time, the existence of potential master regulators coordinating systemic responses to microgravity in rodents. We identified p53, TGF1 and immune related pathways as the highly prevalent pan-tissue signaling pathways that are affected by microgravity. Some variability in the degree of change in their expression across species, strain and time of flight was also observed. Interestingly, while certain skeletal muscle (gastrocnemius and soleus) exhibited an overall down-regulation of these genes, some other muscle types such as the extensor digitorum longus, tibialis anterior and quadriceps, showed an up-regulated expression, indicative of potential compensatory mechanisms to prevent microgravity-induced atrophy. Key genes isolated by unbiased systems analyses displayed a major overlap between tissue types and flight conditions and established TGF1 to be the most connected gene across all data sets. Finally, a set of microgravity responsive miRNA signature was identified and based on their predicted functional state and subsequent impact on health, a theoretical health risk score was calculated. The genes and miRNAs identified from our analyses can be targeted for future research involving efficient countermeasure design. Our study thus exemplifies the utility of GeneLab data repository to aid in the process of performing novel hypothesis based spaceflight research aimed at elucidating the global impact of environmental stressors at multiple biological scales.

In breast cancer subtypes steroid sulfatase (STS) is associated with less aggressive tumour characteristics.

PubMed

McNamara, Keely M; Guestini, Fouzia; Sauer, Torill; Touma, Joel; Bukholm, Ida Rashida; Lindstrøm, Jonas C; Sasano, Hironobu; Geisler, Jürgen

2018-05-01

The majority of breast cancer cases are steroid dependent neoplasms, with hormonal manipulation of either CYP19/aromatase or oestrogen receptor alpha axis being the most common therapy. Alternate pathways of steroid actions are documented, but their interconnections and correlations to BC subtypes and clinical outcome could be further explored. We evaluated selected steroid receptors (Androgen Receptor, Oestrogen Receptor alpha and Beta, Glucocorticoid Receptor) and oestrogen pathways (steroid sulfatase (STS), 17β-hydroxysteroid dehydrogenase 2 (17βHSD2) and aromatase) in a cohort of 139 BC cases from Norway. Using logistic and cox regression analysis, we examined interactions between these and clinical outcomes such as distant metastasis, local relapse and survival. Our principal finding is an impact of STS expression on the risk for distant metastasis (p<0.001) and local relapses (p <0.001), HER2 subtype (p<0.015), and survival (p<0.001). The suggestion of a beneficial effect of alternative oestrogen synthesis pathways was strengthened by inverted, but non-significant findings for 17βHSD2. Increased intratumoural metabolism of oestrogens through STS is associated with significantly lower incidence of relapse and/or distant metastasis and correspondingly improved prognosis. The enrichment of STS in the HER2 overexpressing subtype is intriguing, especially given the possible role of HER-2 over-expression in endocrine resistance.

Phylogeny and Expression Analyses Reveal Important Roles for Plant PKS III Family during the Conquest of Land by Plants and Angiosperm Diversification

PubMed Central

Xie, Lulu; Liu, Pingli; Zhu, Zhixin; Zhang, Shifan; Zhang, Shujiang; Li, Fei; Zhang, Hui; Li, Guoliang; Wei, Yunxiao; Sun, Rifei

2016-01-01

Polyketide synthases (PKSs) utilize the products of primary metabolism to synthesize a wide array of secondary metabolites in both prokaryotic and eukaryotic organisms. PKSs can be grouped into three distinct classes, types I, II, and III, based on enzyme structure, substrate specificity, and catalytic mechanisms. The type III PKS enzymes function as homodimers, and are the only class of PKS that do not require acyl carrier protein. Plant type III PKS enzymes, also known as chalcone synthase (CHS)-like enzymes, are of particular interest due to their functional diversity. In this study, we mined type III PKS gene sequences from the genomes of six aquatic algae and 25 land plants (1 bryophyte, 1 lycophyte, 2 basal angiosperms, 16 core eudicots, and 5 monocots). PKS III sequences were found relatively conserved in all embryophytes, but not exist in algae. We also examined gene expression patterns by analyzing available transcriptome data, and identified potential cis-regulatory elements in upstream sequences. Phylogenetic trees of dicots angiosperms showed that plant type III PKS proteins fall into three clades. Clade A contains CHS/STS-type enzymes coding genes with diverse transcriptional expression patterns and enzymatic functions, while clade B is further divided into subclades b1 and b2, which consist of anther-specific CHS-like enzymes. Differentiation regions, such as amino acids 196-207 between clades A and B, and predicted positive selected sites within α-helixes in late appeared branches of clade A, account for the major diversification in substrate choice and catalytic reaction. The integrity and location of conserved cis-elements containing MYB and bHLH binding sites can affect transcription levels. Potential binding sites for transcription factors such as WRKY, SPL, or AP2/EREBP may contribute to tissue- or taxon-specific differences in gene expression. Our data shows that gene duplications and functional diversification of plant type III PKS enzymes played a critical role in the ancient conquest of the land by early plants and angiosperm diversification. PMID:27625671

Steroid sulphatase and oestrogen sulphotransferase in human non-small-cell lung carcinoma

PubMed Central

Iida, S; Kakinuma, H; Miki, Y; Abe, K; Sakurai, M; Suzuki, S; Niikawa, H; Akahira, J; Suzuki, T; Sasano, H

2013-01-01

Background: Steroid sulphatase (STS) is one of the steroid-metabolising enzymes involved in desulphating inactive steroid sulphates and oestrogen sulphotransferase (EST) sulphates active oestrogen. The roles of both STS and EST have not been examined in oestrogen-dependent non-small-cell lung cancer (NSCLC). Methods: We evaluated the immunoreactivity of STS and EST in NSCLC cases using immunohistochemistry. The function of STS and EST was further demonstrated using NSCLC cell lines. Results: The immunoreactivity of STS and EST was detected in 49.5% and 27.8% of NSCLC cases, respectively. The immunoreactivity of STS was significantly higher in female adenocarcinoma cases. The STS-positive NSCLCs were also significantly correlated in an inversed manner with tumour size and cell proliferation and tended to be associated with better clinical outcome. However, the immunoreactivity of EST was significantly correlated with intracellular oestradiol concentration. Results of in vitro analysis demonstrated that oestrone sulphate (E1-S) induced and pregnenolone sulphate (Preg-S) inhibited the proliferation in STS-expressing cell lines. The inhibition by Preg-S was reversed by a specific progesterone receptor blocker. Simultaneous addition of E1-S and Preg-S significantly suppressed the proliferation. Conclusion: In NSCLC patients, STS is considered a good prognostic factor. Results of our present study also indicated the benefits of potential progesterone therapy for NSCLC patients. PMID:23531699

STS-133 Discovery

NASA Image and Video Library

2010-11-03

The space shuttle Discovery is seen on launch Pad 39a after the Rotating Service Structure (RSS) is rolled back on Wednesday, Nov. 3, 2010 at the NASA Kennedy Space Center in Cape Canaveral, Fla. During space shuttle Discovery's final spaceflight, the STS-133 crew members will take important spare parts to the International Space Station along with the Express Logistics Carrier-4. Photo Credit: (NASA/Bill Ingalls)

KSC-2011-1800

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, employees hold up a banner to commemorate space shuttle Endeavour's STS-134 mission as it is transported from Orbiter Processing Facility-2 to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-1790

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, employees hold up a banner to commemorate space shuttle Endeavour's STS-134 mission as it is transported from Orbiter Processing Facility-2 to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2009-6667

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - At NASA's Kennedy Space Center in Florida, the ExPRESS Logistics Carrier 3, or ELC-3, arrives at the Space Station Processing Facility. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

Growth in spaceflight hardware results in alterations to the transcriptome and proteome.

PubMed

Basu, Proma; Kruse, Colin P S; Luesse, Darron R; Wyatt, Sarah E

2017-11-01

The Biological Research in Canisters (BRIC) hardware has been used to house many biology experiments on both the Space Transport System (STS, commonly known as the space shuttle) and the International Space Station (ISS). However, microscopic examination of Arabidopsis seedlings by Johnson et al. (2015) indicated the hardware itself may affect cell morphology. The experiment herein was designed to assess the effects of the BRIC-Petri Dish Fixation Units (BRIC-PDFU) hardware on the transcriptome and proteome of Arabidopsis seedlings. To our knowledge, this is the first transcriptomic and proteomic comparison of Arabidopsis seedlings grown with and without hardware. Arabidopsis thaliana wild-type Columbia (Col-0) seeds were sterilized and bulk plated on forty-four 60 mm Petri plates, of which 22 were integrated into the BRIC-PDFU hardware and 22 were maintained in closed containers at Ohio University. Seedlings were grown for approximately 3 days, fixed with RNAlater ® and stored at -80 °C prior to RNA and protein extraction, with proteins separated into membrane and soluble fractions prior to analysis. The RNAseq analysis identified 1651 differentially expressed genes; MS/MS analysis identified 598 soluble and 589 membrane proteins differentially abundant both at p < .05. Fold enrichment analysis of gene ontology terms related to differentially expressed transcripts and proteins highlighted a variety of stress responses. Some of these genes and proteins have been previously identified in spaceflight experiments, indicating that these genes and proteins may be perturbed by both conditions. Copyright © 2017 The Committee on Space Research (COSPAR). Published by Elsevier Ltd. All rights reserved.

STS-126 crew visit

NASA Image and Video Library

2009-01-13

Stennis Space Center Director Gene Goldman (center) stands with astronauts Christopher Ferguson (right) and Heidemarie Stefanyshyn-Piper in front of the A-2 Test Stand during the space shuttle crew members' visit to NASA's rocket engine testing facility Jan. 13. During their visit, Ferguson and Stefanyshyn-Piper reported on the STS-126 space shuttle delivery and servicing mission to the International Space Station. Ferguson served as commander of the mission. Stefanyshyn-Piper served as a mission specialist.

STS-126 crew visit

NASA Technical Reports Server (NTRS)

2009-01-01

Stennis Space Center Director Gene Goldman (center) stands with astronauts Christopher Ferguson (right) and Heidemarie Stefanyshyn-Piper in front of the A-2 Test Stand during the space shuttle crew members' visit to NASA's rocket engine testing facility Jan. 13. During their visit, Ferguson and Stefanyshyn-Piper reported on the STS-126 space shuttle delivery and servicing mission to the International Space Station. Ferguson served as commander of the mission. Stefanyshyn-Piper served as a mission specialist.

Segregation of genes from donor strain during the production of recombinant congenic strains.

PubMed

van Zutphen, L F; Den Bieman, M; Lankhorst, A; Demant, P

1991-07-01

Recombinant congenic strains (RCS) constitute a set of inbred strains which are designed to dissect the genetic control of multigenic traits, such as tumour susceptibility or disease resistance. Each RCS contains a small fraction of the genome of a common donor strain, while the majority of genes stem from a common background strain. We tested at two stages of the inbreeding process in 20 RCS, derived from BALB/cHeA and STS/A, to see whether alleles from the STS/A donor strain are distributed over the RCS in a ratio as would theoretically be expected. Four marker genes (Pep-3; Pgm-1; Gpi-1 and Es-3) located at 4 different chromosomes were selected and the allelic distribution was tested after 3-4 and after 12 generations of inbreeding. The data obtained do not significantly deviate from the expected pattern, thus supporting the validity of the concept of RCS.

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site watch as space shuttle Atlantis springs into action from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Like a phoenix rising from the flames, space shuttle Atlantis takes flight from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - An exhaust cloud begins to form around space shuttle Atlantis as it springs into action from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit:Jim Grossmann

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Space shuttle Atlantis cuts its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-1798

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, STS-134 Pilot Gregory H. Johnson and Mission Specialist Roberto Vittori with the European Space Agency accompany space shuttle Endeavour's move, or "rollover," to the Vehicle Assembly Building (VAB). In the VAB, Endeavour will be lifted into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final and upcoming STS-134 mission. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

Vibrio parahaemolyticus isolates from southeastern Chinese coast are genetically diverse with circulation of clonal complex 3 strains since 2002.

PubMed

Yu, Ying; Hu, Weizhao; Wu, Beibei; Zhang, Peipei; Chen, Jianshun; Wang, Shuna; Fang, Weihuan

2011-11-01

Multilocus sequence typing (MLST) was used to examine the clonal relationship and genetic diversity of 71 Vibrio parahaemolyticus isolates from clinical and seafood-related sources in southeastern Chinese coast between 2002 and 2009. The tested isolates fell into 61 sequence types (STs). Of 17 clinical isolates, 7 belonged to ST3 of the pandemic clonal complex 3, with 3 strains isolated in 2002. Although there was no apparent clonal relationship found between clinical strains and those from seafood-related sources positive with pathogenic markers, there were clonal relationships between clinical strains from this study and those from environmental sources in other parts of China. Phylogenetic analysis showed that strains of 112 STs (61 STs from this study and 51 retrieved from PUBMLST database covering different continents) could be divided into four branches. The vast majority of our isolates and those from other countries were genetically diverse and clustered into two major branches of mixed distribution (of geographic origins and sample sources), whereas five STs representing six isolates split as two minor branches because of divergence of their recA genes, which had 80%-82% nucleotide identity to typical V. parahaemolyticus strains and 73.3%-76.9% identity to the CDS24 of a Vibrio sp. plasmid p23023, indicating that the recA gene might have recombined by lateral gene transfer. This was further supported by a high ratio of recombination to mutation (3.038) for recA. In conclusion, MLST with fully extractable database is a powerful system for analysis of clonal relationship for strains of a particular region in a national or global scale as well as between clinical and environmental or food-related strains.

Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq

PubMed Central

Wilson, J. W.; Ott, C. M.; zu Bentrup, K. Höner; Ramamurthy, R.; Quick, L.; Porwollik, S.; Cheng, P.; McClelland, M.; Tsaprailis, G.; Radabaugh, T.; Hunt, A.; Fernandez, D.; Richter, E.; Shah, M.; Kilcoyne, M.; Joshi, L.; Nelman-Gonzalez, M.; Hing, S.; Parra, M.; Dumars, P.; Norwood, K.; Bober, R.; Devich, J.; Ruggles, A.; Goulart, C.; Rupert, M.; Stodieck, L.; Stafford, P.; Catella, L.; Schurr, M. J.; Buchanan, K.; Morici, L.; McCracken, J.; Allen, P.; Baker-Coleman, C.; Hammond, T.; Vogel, J.; Nelson, R.; Pierson, D. L.; Stefanyshyn-Piper, H. M.; Nickerson, C. A.

2007-01-01

A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth. PMID:17901201

Altered Gravity Induces Oxidative Stress in Drosophila Melanogaster

NASA Technical Reports Server (NTRS)

Bhattacharya, Sharmila; Hosamani, Ravikumar

2015-01-01

Altered gravity environments can induce increased oxidative stress in biological systems. Microarray data from our previous spaceflight experiment (FIT experiment on STS-121) indicated significant changes in the expression of oxidative stress genes in adult fruit flies after spaceflight. Currently, our lab is focused on elucidating the role of hypergravity-induced oxidative stress and its impact on the nervous system in Drosophila melanogaster. Biochemical, molecular, and genetic approaches were combined to study this effect on the ground. Adult flies (2-3 days old) exposed to acute hypergravity (3g, for 1 hour and 2 hours) showed significantly elevated levels of Reactive Oxygen Species (ROS) in fly brains compared to control samples. This data was supported by significant changes in mRNA expression of specific oxidative stress and antioxidant defense related genes. As anticipated, a stress-resistant mutant line, Indy302, was less vulnerable to hypergravity-induced oxidative stress compared to wild-type flies. Survival curves were generated to study the combined effect of hypergravity and pro-oxidant treatment. Interestingly, many of the oxidative stress changes that were measured in flies showed sex specific differences. Collectively, our data demonstrate that altered gravity significantly induces oxidative stress in Drosophila, and that one of the organs where this effect is evident is the brain.

STS-121: Discovery Post Landing Press Conference

NASA Technical Reports Server (NTRS)

2006-01-01

On July 17, 2006 Dean Acosta (NASA Press Secretary), Mike Griffin (Administrator), Bill Gerstenmaier (Associate Administrator of Space Operations), and Mike Leinbach (NASA Launch Director) expressed how proud they were to be a part of the STS-121/ Discovery team. They also explained how flawlessly the mission performed and how it was the best mission ever flown. They proceeded to answer numerous questions from the press.

Gene-specific sex effects on eosinophil infiltration in leishmaniasis.

PubMed

Slapničková, Martina; Volkova, Valeriya; Čepičková, Marie; Kobets, Tatyana; Šíma, Matyáš; Svobodová, Milena; Demant, Peter; Lipoldová, Marie

2016-01-01

Sex influences susceptibility to many infectious diseases, including some manifestations of leishmaniasis. The disease is caused by parasites that enter to the skin and can spread to the lymph nodes, spleen, liver, bone marrow, and sometimes lungs. Parasites induce host defenses including cell infiltration, leading to protective or ineffective inflammation. These responses are often influenced by host genotype and sex. We analyzed the role of sex in the impact of specific gene loci on eosinophil infiltration and its functional relevance. We studied the genetic control of infiltration of eosinophils into the inguinal lymph nodes after 8 weeks of Leishmania major infection using mouse strains BALB/c, STS, and recombinant congenic strains CcS-1,-3,-4,-5,-7,-9,-11,-12,-15,-16,-18, and -20, each of which contains a different random set of 12.5% genes from the parental "donor" strain STS and 87.5% genes from the "background" strain BALB/c. Numbers of eosinophils were counted in hematoxylin-eosin-stained sections of the inguinal lymph nodes under a light microscope. Parasite load was determined using PCR-ELISA. The lymph nodes of resistant STS and susceptible BALB/c mice contained very low and intermediate numbers of eosinophils, respectively. Unexpectedly, eosinophil infiltration in strain CcS-9 exceeded that in BALB/c and STS and was higher in males than in females. We searched for genes controlling high eosinophil infiltration in CcS-9 mice by linkage analysis in F 2 hybrids between BALB/c and CcS-9 and detected four loci controlling eosinophil numbers. Lmr14 (chromosome 2) and Lmr25 (chromosome 5) operate independently from other genes (main effects). Lmr14 functions only in males, the effect of Lmr25 is sex independent. Lmr15 (chromosome 11) and Lmr26 (chromosome 9) operate in cooperation (non-additive interaction) with each other. This interaction was significant in males only, but sex-marker interaction was not significant. Eosinophil infiltration was positively correlated with parasite load in lymph nodes of F 2 hybrids in males, but not in females. We demonstrated a strong influence of sex on numbers of eosinophils in the lymph nodes after L. major infection and present the first identification of sex-dependent autosomal loci controlling eosinophilic infiltration. The positive correlation between eosinophil infiltration and parasite load in males suggests that this sex-dependent eosinophilic infiltration reflects ineffective inflammation.

Complex Actions of Estradiol-3-Sulfate in Late Gestation Fetal Brain

PubMed Central

Winikor, Jared; Schlaerth, Christine; Rabaglino, Maria Belen; Cousins, Roderick; Sutherland, Monique

2011-01-01

The most abundant form of estrogen circulating in fetal plasma is sulfo-conjugated estrogen; for example, estradiol-3-sulfate (E2SO4) is more highly abundant than estradiol (E2). The present study investigated the ontogeny of the deconjugating (steroid sulfatase [STS]) and conjugating (estrogen sulfotransferase [STF]) enzymes in ovine fetal brain and tested the hypothesis that treatment with E2SO4 would alter the expression of one or both enzymes. Steroid sulfatase was more highly expressed than STF, and both changed as a function of gestational age. Estradiol-3-sulfate infused intracerebroventricularly (icv) significantly increased plasma adrenocorticotropic hormone (ACTH) and cortisol concentrations. Plasma E2 and E2SO4 were increased, and brain expression of estrogen receptor α was decreased. The proteins STS and STF were up- and downregulated, respectively. Pituitary proopiomelanocortin (POMC) and follicle-stimulating hormone (FSH) and hypothalamic corticotrophin-releasing hormone (CRH) messenger RNA (mRNA) was decreased. We conclude that E2SO4 has complex actions on the fetal brain, which might involve deconjugation by STS, but that the net result of direct E2SO4 icv infusion is more complex than can be accounted for by infusion of E2 alone. PMID:21273638

Spaceflight Effects and Molecular Responses in the Mouse Eye: Observations after NASA Shuttle Mission STS-133

NASA Technical Reports Server (NTRS)

ProsperoPonce, Claudia Maria; Zanello, Susana B.; Theriot, Corey A.; Chevez-Barrios, Patricia

2012-01-01

Background: Human space exploration implies a combination of stressors including microgravityinduced cephalad fluid shift and radiation exposure. Ocular changes in astronauts leading to visual impairment are of occupational health relevance. The effect of this complex environment on ocular morphology and function is poorly understood. Material and Methods: Mice were assigned to a Flight (FLT) group flown on shuttle mission STS133, Animal Enclosure Module (AEM), or vivarium (VIV) ground controls. Eyes were collected at 1, 5 and 7 days after landing, and were fixed for histological sectioning. The contralateral eye was used for gene expression profiling by qRT-PCR. Routine histology and immunohistochemistry using 8-hydroxy-2'-deoxyguanosine (8-OHdG), caspase-3, glial fibrillary acidic protein (GFAP) and beta-amyloid were used to study the eyes. Results and Conclusions: 8-OHdG and caspase-3 immunoreactivity was increased in the retina in FLT samples at return from flight (R+1) compared to ground controls, and decreased at day 7 (R+7), suggesting an increase in oxidative stress and cell apoptosis. FLT mice showed evidence of retinal pigment epithelium (RPE) apoptosis possibly secondary to oxidative damage. Although attenuation of RPE has been related to retinal choroidal folds in astronauts, it is yet to be determined whether or not increased RPE apoptosis may contribute to the formation of choroidal folds or may increase the risk for other retinal pathologies, such as AMD. beta-amyloid was seen in the nerve fibers at the post-laminar region of the optic nerve in the flight samples (R+7). Deposition of beta-amyloid has a strong correlation with mechanical trauma. The coexpression of GFAP in astrocytes and oligodentrocytes in these same areas supports the possible mechanical origin probably secondary to intracranial pressure that is transmitted into the nerve, as a result of an increase in venous pressure associated to microgravity-induced cephalic fluid shift. However, there is the need to further investigate the nature of the changes through additional experimental work. Gene expression of oxidative and cellular stress response genes was unregulated in the retina of FLT samples upon landing followed by lower levels by R+7. These results suggest that reversible molecular damage occurs in the retina of mice exposed to spaceflight and that protective cellular and molecular pathways are induced in the retina in response to these changes.

Deletion patterns of the STS gene and flanking sequences in Israeli X-linked ichthyosis patients and carriers: analysis by polymerase chain reaction and fluorescence in situ hybridization techniques.

PubMed

Aviram-Goldring, A; Goldman, B; Netanelov-Shapira, I; Chen-Shtoyerman, R; Zvulunov, A; Tal, O; Ilan, T; Peleg, L

2000-03-01

Deletion of the entire steroid sulfatase (STS) gene is the most common molecular defect in X-linked ichthyosis (XLI) patients. Usually, additional flanking sequences are also missing. The aim of this study was to estimate the extent of deletions in an ethnically heterogeneous population of Israeli XLI patients. Multiplex polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques were applied in the analysis of blood samples of 24 patients and amniotic cells of seven affected fetuses from 22 unrelated families. In 19 families, a large deletion of the 2-3 megabase was found. It included the whole STS gene and spanned adjacent areas up- and downstream between the loci DXS 1139 and DXS 1132. Two unrelated families of Iraqi ancestry had a partial deletion of the gene and its centromeric adjacent sequence. In another family, the telomeric end of the extragenic segment was only partially missing. Application of FISH on metaphase blood cells and interphase amniotic cells confirmed the diagnosis of XLI in all patients, except the three with partial intragenic deletion. In those cases, the remaining fraction of the gene was sufficient to provide a false negative result. Diagnosis of carriers and prenatal diagnosis in uncultured cells was applicable only by FISH. Our study revealed a remarkable heterogeneity in the deletion pattern among Israeli patients with XLI. This heterogeneity could not be attributed to specific ethnic groups because of the small size of the study group. More studies involving patients of various ancestries should be carried out. In addition, this study demonstrated the usefulness of the FISH technique in the prenatal diagnosis of fetuses with suspected XLI.

STA Flying Weather Reconnaissance / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-067 (1 June 2011) --- Astronaut Rick Sturckow flies weather reconnaissance in a Shuttle Training Aircraft over NASA's Kennedy Space Center in Florida to assess conditions before space shuttle Endeavour returns to Earth for the final time. Weather was observed "go" and Endeavour glided to a stop on the Shuttle Landing Facility's Runway 15 at 2:35 a.m. EDT, bringing an end to the STS-134 mission. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

A chain reaction approach to modelling gene pathways.

PubMed

Cheng, Gary C; Chen, Dung-Tsa; Chen, James J; Soong, Seng-Jaw; Lamartiniere, Coral; Barnes, Stephen

2012-08-01

BACKGROUND: Of great interest in cancer prevention is how nutrient components affect gene pathways associated with the physiological events of puberty. Nutrient-gene interactions may cause changes in breast or prostate cells and, therefore, may result in cancer risk later in life. Analysis of gene pathways can lead to insights about nutrient-gene interactions and the development of more effective prevention approaches to reduce cancer risk. To date, researchers have relied heavily upon experimental assays (such as microarray analysis, etc.) to identify genes and their associated pathways that are affected by nutrient and diets. However, the vast number of genes and combinations of gene pathways, coupled with the expense of the experimental analyses, has delayed the progress of gene-pathway research. The development of an analytical approach based on available test data could greatly benefit the evaluation of gene pathways, and thus advance the study of nutrient-gene interactions in cancer prevention. In the present study, we have proposed a chain reaction model to simulate gene pathways, in which the gene expression changes through the pathway are represented by the species undergoing a set of chemical reactions. We have also developed a numerical tool to solve for the species changes due to the chain reactions over time. Through this approach we can examine the impact of nutrient-containing diets on the gene pathway; moreover, transformation of genes over time with a nutrient treatment can be observed numerically, which is very difficult to achieve experimentally. We apply this approach to microarray analysis data from an experiment which involved the effects of three polyphenols (nutrient treatments), epigallo-catechin-3-O-gallate (EGCG), genistein, and resveratrol, in a study of nutrient-gene interaction in the estrogen synthesis pathway during puberty. RESULTS: In this preliminary study, the estrogen synthesis pathway was simulated by a chain reaction model. By applying it to microarray data, the chain reaction model computed a set of reaction rates to examine the effects of three polyphenols (EGCG, genistein, and resveratrol) on gene expression in this pathway during puberty. We first performed statistical analysis to test the time factor on the estrogen synthesis pathway. Global tests were used to evaluate an overall gene expression change during puberty for each experimental group. Then, a chain reaction model was employed to simulate the estrogen synthesis pathway. Specifically, the model computed the reaction rates in a set of ordinary differential equations to describe interactions between genes in the pathway (A reaction rate K of A to B represents gene A will induce gene B per unit at a rate of K; we give details in the "method" section). Since disparate changes of gene expression may cause numerical error problems in solving these differential equations, we used an implicit scheme to address this issue. We first applied the chain reaction model to obtain the reaction rates for the control group. A sensitivity study was conducted to evaluate how well the model fits to the control group data at Day 50. Results showed a small bias and mean square error. These observations indicated the model is robust to low random noises and has a good fit for the control group. Then the chain reaction model derived from the control group data was used to predict gene expression at Day 50 for the three polyphenol groups. If these nutrients affect the estrogen synthesis pathways during puberty, we expect discrepancy between observed and expected expressions. Results indicated some genes had large differences in the EGCG (e.g., Hsd3b and Sts) and the resveratrol (e.g., Hsd3b and Hrmt12) groups. CONCLUSIONS: In the present study, we have presented (I) experimental studies of the effect of nutrient diets on the gene expression changes in a selected estrogen synthesis pathway. This experiment is valuable because it allows us to examine how the nutrient-containing diets regulate gene expression in the estrogen synthesis pathway during puberty; (II) global tests to assess an overall association of this particular pathway with time factor by utilizing generalized linear models to analyze microarray data; and (III) a chain reaction model to simulate the pathway. This is a novel application because we are able to translate the gene pathway into the chemical reactions in which each reaction channel describes gene-gene relationship in the pathway. In the chain reaction model, the implicit scheme is employed to efficiently solve the differential equations. Data analysis results show the proposed model is capable of predicting gene expression changes and demonstrating the effect of nutrient-containing diets on gene expression changes in the pathway. One of the objectives of this study is to explore and develop a numerical approach for simulating the gene expression change so that it can be applied and calibrated when the data of more time slices are available, and thus can be used to interpolate the expression change at a desired time point without conducting expensive experiments for a large amount of time points. Hence, we are not claiming this is either essential or the most efficient way for simulating this problem, rather a mathematical/numerical approach that can model the expression change of a large set of genes of a complex pathway. In addition, we understand the limitation of this experiment and realize that it is still far from being a complete model of predicting nutrient-gene interactions. The reason is that in the present model, the reaction rates were estimated based on available data at two time points; hence, the gene expression change is dependent upon the reaction rates and a linear function of the gene expressions. More data sets containing gene expression at various time slices are needed in order to improve the present model so that a non-linear variation of gene expression changes at different time can be predicted.

Substantial prevalence of microdeletions of the Y-chromosome in infertile men with idiopathic azoospermia and oligozoospermia detected using a sequence-tagged site-based mapping strategy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Najmabadi, H.; Huang, V.; Bhasin, D.

1996-04-01

Genes on the long arm of Y (Yq), particularly within interval 6, are believed to play a critical role in human spermatogenesis. Cytogenetically detectable deletions of this region are associated with azoospermia in men, but are relatively uncommon. The objective of this study was to validate a sequence-tagged site (STS)-mapping strategy for the detection of Yq microdeletions and to use this method to determine the proportion of men with idiopathic azoospermia or severe oligozoospermia who carry microdeletions in Yq. STS mapping of a sufficiently large sample of infertile men should also help further localize the putative gene(s) involved in themore » pathogenesis of male infertility. Genomic DNA was extracted from peripheral leukocytes of 16 normal fertile men, 7 normal fertile women, 60 infertile men, and 15 patients with the X-linked disorder, ichthyosis. PCR primers were synthesized for 26 STSs that span Yq interval 6. None of the 16 normal men of known fertility had microdeletions. Seven normal fertile women failed to amplify any of the 26 STSs, providing evidence of their Y specificity. No microdeletions were detected in any of the 15 patients with ichthyosis. Of the 60 infertile men typed with 26 STSs, 11 (18%; 10 azoospermic and 1 oligozoospermic) failed to amplify 1 or more STS. Interestingly, 4 of the 11 patients had microdeletions in a region that is outside the Yq region from which the DAZ (deleted in azoospermia gene region) gene was cloned. In an additional 3 patients, microdeletions were present both inside and outside the DAZ region. The physical locations of these microdeletions provide further support for the concept that a gene(s) on Yq deletion interval 6 plays an important role in spermatogenesis. The presence of deletions that do not overlap with the DAZ region suggests that genes other than the DAZ gene may also be implicated in the pathogenesis of some subsets of male infertility. 48 refs., 2 figs., 2 tabs.« less

KSC-2009-6662

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - At NASA's Kennedy Space Center in Florida, U.S. Air Force C-5 aircraft lands at the Shuttle Landing Facility, delivering the ExPRESS Logistics Carrier 3, or ELC-3. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2009-6671

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, workers unwrap the ExPRESS Logistics Carrier 3, or ELC-3, still nestled in its transportation case. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2009-6670

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, workers supervise the uncrating of the ExPRESS Logistics Carrier 3, or ELC-3. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2009-6664

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - At the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, the ExPRESS Logistics Carrier 3, or ELC-3, is offloaded from a U.S. Air Force C-5 aircraft. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2009-6673

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, the newly arrived ExPRESS Logistics Carrier 3, or ELC-3, is lifted high above the clean room floor. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2009-6668

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, preparations are under way to uncrate the ExPRESS Logistics Carrier 3, or ELC-3. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2011-1789

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, employees gather to hold up a banner to commemorate space shuttle Endeavour's STS-134 mission, as it is moved from Orbiter Processing Facility-2 to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-2515

NASA Image and Video Library

2011-03-29

CAPE CANAVERAL, Fla. -- STS-134 Mission Specialist Andrew Feustel listens to Commander Mark Kelly address the media on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. While at Kennedy, space shuttle Endeavour's crew will participate in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training in preparation for the upcoming STS-134 mission. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-2518

NASA Image and Video Library

2011-03-29

CAPE CANAVERAL, Fla. -- STS-134 Pilot Greg H. Johnson listens to Commander Mark Kelly address the media on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. While at Kennedy, space shuttle Endeavour's crew will participate in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training in preparation for the upcoming STS-134 mission. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-2502

NASA Image and Video Library

2011-03-29

CAPE CANAVERAL, Fla. -- STS-134 Pilot Greg H. Johnson stands in front of a T-38 jet on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. While at Kennedy, space shuttle Endeavour's crew will participate in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training in preparation for the upcoming STS-134 mission. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-2514

NASA Image and Video Library

2011-03-29

CAPE CANAVERAL, Fla. -- STS-134 Mission Specialist Greg Chamitoff listens to Commander Mark Kelly address the media on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. While at Kennedy, space shuttle Endeavour's crew will participate in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training in preparation for the upcoming STS-134 mission. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3585

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Media capture the launch of space shuttle Endeavour on the STS-134 mission to the International Space Station from the Press Site at NASA's Kennedy Space Center in Florida. The shuttle and its six-member crew lifted off on time at 8:56 a.m. EDT on May 16. STS-134 will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

KSC-2011-3586

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- The American flag sways in the breeze as space shuttle Endeavour launches on the STS-134 mission to the International Space Station. The shuttle and its six-member crew lifted off from Launch Pad 39A at NASA's Kennedy Space Center in Florida on time at 8:56 a.m. EDT on May 16. STS-134 will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

The neuroprotective action of the mood stabilizing drugs lithium chloride and sodium valproate is mediated through the up-regulation of the homeodomain protein Six1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Plant, Kathryn E.; Anderson, Elizabeth; Simecek, Nicole

2009-02-15

The mood stabilizing agents lithium chloride (LiCl) and sodium valproate (VPA) have recently gained interest as potential neuroprotective therapeutics. However, exploitation of these therapeutic applications is hindered by both a lack of molecular understanding of the mode of action, and a number of sub-optimal properties, including a relatively small therapeutic window and variable patient response. Human neuroblastoma cells (SH-SY5Y) were exposed to 1 mM lithium chloride or 1 mM sodium valproate for 6 h or 72 h, and transcriptomes measured by Affymetrix U133A/B microarray. Statistically significant gene expression changes were identified using SAM software, with selected changes confirmed at transcriptmore » (TaqMan) and protein (Western blotting) levels. Finally, anti-apoptotic action was measured by an in vitro fluorescent assay. Exposure of SH-SY5Y cells to therapeutically relevant concentrations of either lithium chloride or sodium valproate elicited 936 statistically significant changes in gene expression. Amongst these changes we observed a large (maximal 31.3-fold) increase in the expression of the homeodomain protein Six1, and have characterized the time- and dose-dependent up-regulation of this gene in response to both drugs. In addition, we demonstrate that, like LiCl or VPA treatment, Six1 over-expression protects SH-SY5Y cells from staurosporine-induced apoptosis via the blockade of caspsase-3 activation, whereas removal of Six1 protein via siRNA antagonises the ability of LiCl and VPA to protect SH-SY5Y cells from STS-induced apoptosis. These results provide a novel mechanistic rationale underlying the neuroprotective mechanism of LiCl and VPA, suggesting exciting possibilities for the development of novel therapeutic agents against neurodegenerative diseases such as Alzheimer's or Parkinsonism.« less

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - With nearly 7 million pounds of thrust generated by twin solid rocket boosters and three main engines, space shuttle Atlantis zooms into the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kenny Allen

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - With landing gear down, space shuttle Atlantis approaches landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site witness space shuttle Atlantis cut its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - With nearly 7 million pounds of thrust generated by twin solid rocket boosters and three main engines, space shuttle Atlantis races to orbit over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kenny Allen

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - With drag chute unfurled, space shuttle Atlantis lands on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site have front-row seats as space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls to slow space shuttle Atlantis for landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Sandra Joseph

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls as space shuttle Atlantis lands on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Space shuttle Atlantis touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls to slow space shuttle Atlantis for landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Space shuttle Atlantis kicks up dust as it touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Streams of smoke trail from the main landing gear tires as space shuttle Atlantis touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million-mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

Toward the Reconstitution of a Two-Enzyme Cascade for Resveratrol Synthesis on Potyvirus Particles.

PubMed

Besong-Ndika, Jane; Wahlsten, Matti; Cardinale, Daniela; Pille, Jan; Walter, Jocelyne; Michon, Thierry; Mäkinen, Kristiina

2016-01-01

The highly ordered protein backbone of virus particles makes them attractive candidates for use as enzyme nano-carriers (ENCs). We have previously developed a non-covalent and versatile approach for adhesion of enzymes to virus particles. This approach makes use of z33, a peptide derived from the B-domain of Staphylococcus aureus protein A, which binds to the Fc domain of many immunoglobulins. We have demonstrated that with specific antibodies addressed against the viral capsid proteins (CPs) an 87% coverage of z33-tagged proteins can be achieved on potyvirus particles. 4-coumarate coenzyme A ligase (4CL2) and stilbene synthase (STS) catalyze consecutive steps in the resveratrol synthetic pathway. In this study, these enzymes were modified to carry an N-terminal z33 peptide and a C-terminal 6xHis tag to obtain (z)4CL2(His) and (z)STS(His), respectively. A protein chimera, (z)4CL2::STS(His), with the same modifications was also generated from the genetic fusion of both mono-enzyme encoding genes. All z33 enzymes were biologically active after expression in Escherichia coli as revealed by LC-MS analysis to identify resveratrol and assembled readily into macromolecular complexes with Potato virus A particles and α-PVA CP antibodies. To test simultaneous immobilization-purification, we applied the double antibody sandwich - ELISA protocol to capture active z33-containg mono-enzymes and protein chimera directly from clarified soluble cell lysates onto the virus particle surface. These immobilized enzymes were able to synthesize resveratrol. We present here a bottom up approach to immobilize active enzymes onto virus-based ENCs and discuss the potential to utilize this method in the purification and configuration of nano-devices.

Efficacy of Sunitinib and Radiotherapy in Genetically Engineered Mouse Model of Soft-Tissue Sarcoma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yoon, Sam S.; Stangenberg, Lars; Lee, Yoon-Jin

Purpose: Sunitinib (SU) is a multitargeted receptor tyrosine kinase inhibitor of the vascular endothelial growth factor and platelet-derived growth factor receptors. The present study examined SU and radiotherapy (RT) in a genetically engineered mouse model of soft tissue sarcoma (STS). Methods and Materials: Primary extremity STSs were generated in genetically engineered mice. The mice were randomized to treatment with SU, RT (10 Gy x 2), or both (SU+RT). Changes in the tumor vasculature before and after treatment were assessed in vivo using fluorescence-mediated tomography. The control and treated tumors were harvested and extensively analyzed. Results: The mean fluorescence in themore » tumors was not decreased by RT but decreased 38-44% in tumors treated with SU or SU+RT. The control tumors grew to a mean of 1378 mm{sup 3} after 12 days. SU alone or RT alone delayed tumor growth by 56% and 41%, respectively, but maximal growth inhibition (71%) was observed with the combination therapy. SU target effects were confirmed by loss of target receptor phosphorylation and alterations in SU-related gene expression. Cancer cell proliferation was decreased and apoptosis increased in the SU and RT groups, with a synergistic effect on apoptosis observed in the SU+RT group. RT had a minimal effect on the tumor microvessel density and endothelial cell-specific apoptosis, but SU alone or SU+RT decreased the microvessel density by >66% and induced significant endothelial cell apoptosis. Conclusion: SU inhibited STS growth by effects on both cancer cells and tumor vasculature. SU also augmented the efficacy of RT, suggesting that this combination strategy could improve local control of STS.« less

Gravity resistance, another graviresponse in plants - role of microtubule-membrane-cell wall continuum

NASA Astrophysics Data System (ADS)

Hoson, T.; Saito, Y.; Usui, S.; Soga, K.; Wakabayashi, K.

Resistance to the gravitational force has been a serious problem for plants to survive on land, after they first went ashore more than 400 million years ago. Thus, gravity resistance is the principal graviresponse in plants comparable to gravitropism. Nevertheless, only limited information has been obtained for this second gravity response. We have examined the mechanism of gravity resistance using hypergravity conditions produced by centrifugation. The results led a hypothesis on the mechanism of plant resistance to the gravitational force that the plant constructs a tough body by increasing the cell wall rigidity, which are brought about by modification of the cell wall metabolism and cell wall environment, especially pH. The hypothesis was further supported by space experiments during the Space Shuttle STS-95 mission. On the other hand, we have shown that gravity signal may be perceived by mechanoreceptors (mechanosensitive ion channels) on the plasma membrane and amyloplast sedimentation in statocytes is not involved in gravity resistance. Moreover, hypergravity treatment increased the expression levels of genes encoding alpha-tubulin, a component of microtubules and 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGR), which catalyzes a reaction producing mevalonic acid, a key precursor of terpenoids such as membrane sterols. The expression of HMGR and alpha- and beta-tubulin genes increased within several hours after hypergravity treatment, depending on the magnitude of gravity. The determination of levels of gene products as well as the analysis with knockout mutants of these genes by T-DNA insertions in Arabidopsis supports the involvement of both membrane sterols and microtubules in gravity resistance. These results suggest that structural or physiological continuum of microtubule-cell membrane-cell wall is responsible for plant resistance to the gravitational force.

STS-133 Discovery

NASA Image and Video Library

2010-11-03

An faint profile outline of the space shuttle Discovery and launch pad 39a are seen projected in the sky as powerful xenon lights illuminate launch pad 39a on Wednesday, Nov. 3, 2010 at the NASA Kennedy Space Center in Cape Canaveral, Fla. During space shuttle Discovery's final spaceflight, the STS-133 crew members will take important spare parts to the International Space Station along with the Express Logistics Carrier-4. Photo Credit: (NASA/Bill Ingalls)

Differential induction of antioxidant stilbenoids in hairy roots of Vitis rotundifolia treated with methyl jasmonate and hydrogen peroxide.

PubMed

Nopo-Olazabal, Cesar; Condori, Jose; Nopo-Olazabal, Luis; Medina-Bolivar, Fabricio

2014-01-01

Stilbenoids are polyphenolic phytoalexins that exhibit potential health applications in humans. Hairy root cultures of muscadine grape (Vitis rotundifolia Michx.) were used to study the biochemical and molecular regulation of stilbenoid biosynthesis upon treatment with 100 μM methyl jasmonate (MeJA) or 10 mM hydrogen peroxide (H2O2) over a 96-h period. Resveratrol, piceid, and ε-viniferin were identified in higher concentrations in the tissue whereas resveratrol was the most abundant stilbenoid in the medium under either treatment. An earlier increase in resveratrol accumulation was observed for the MeJA-treated group showing a maximum at 12 h in the tissue and 18 h in the medium. Furthermore, the antioxidant capacity of extracts from the tissue and medium was determined by the 2,2'-azinobis[3-ethylbenzthiazoline sulfonic acid] (ABTS) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays showing correlation with the stilbenoid content. Fourteen candidate reference genes for qPCR were tested under the described experimental conditions and resulted in the selection of 5 reference genes. Quantitative analyses of transcripts for phenylalanine ammonia-lyase (PAL), resveratrol synthase (RS), and two stilbene synthases (STS and STS2) showed the highest RNA level induction at 3 h for both treatments with a higher induction for the MeJA treatment. In contrast, the flavonoid-related chalcone synthase (CHS) transcripts showed induction and a decrease in expression for MeJA and H2O2 treatments, respectively. The observed responses could be related to an oxidative burst triggered by the exposure to abiotic stressor compounds with signaling function such as MeJA and H2O2 which have been previously related to the synthesis of secondary metabolites. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

KSC-2011-2732

NASA Image and Video Library

2011-04-01

CAPE CANAVERAL, Fla. -- STS-134 Commander Mark Kelly prepares to depart the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida aboard a T-38 jet. While at Kennedy, space shuttle Endeavour's crew participated in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training. Kelly will return to NASA's Johnson Space Center in Houston to resume training for the upcoming STS-134 mission. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 29 at 3:47 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-2503

NASA Image and Video Library

2011-03-29

CAPE CANAVERAL, Fla. -- Shuttle Launch Director Mike Leinbach, right, is on hand to greet STS-134 Mission Specialist Andrew Feustel who arrived on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida aboard a T-38 jet. While at Kennedy, space shuttle Endeavour's crew will participate in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training in preparation for the upcoming STS-134 mission. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-2513

NASA Image and Video Library

2011-03-29

CAPE CANAVERAL, Fla. -- STS-134 Mission Specialist Roberto Vittori, with the European Space Agency, listens to Commander Mark Kelly address the media on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. While at Kennedy, space shuttle Endeavour's crew will participate in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training in preparation for the upcoming STS-134 mission. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-2731

NASA Image and Video Library

2011-04-01

CAPE CANAVERAL, Fla. -- STS-134 Mission Specialist Michael Fincke prepares to depart the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida aboard a T-38 jet. While at Kennedy, space shuttle Endeavour's crew participated in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training. Fincke will return to NASA's Johnson Space Center in Houston to resume training for the upcoming STS-134 mission. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 29 at 3:47 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3587

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- The American flag sways in the breeze as space shuttle Endeavour soars into the sky on the STS-134 mission to the International Space Station. The shuttle and its six-member crew lifted off from Launch Pad 39A at NASA's Kennedy Space Center in Florida on time at 8:56 a.m. EDT on May 16. STS-134 will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

KSC-2009-6672

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, workers attach a crane to the ExPRESS Logistics Carrier 3, or ELC-3, in preparations to lift it from its transportation case. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2009-6663

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - At the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, preparations are under way to offload the ExPRESS Logistics Carrier 3, or ELC-3, from a U.S. Air Force C-5 aircraft. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2009-6675

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, the newly arrived ExPRESS Logistics Carrier 3, or ELC-3, is lowered onto a work stand in the clean room. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

KSC-2009-6665

NASA Image and Video Library

2009-12-06

CAPE CANAVERAL, Fla. - At the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, the ExPRESS Logistics Carrier 3, or ELC-3, is removed from the cargo compartment of a U.S. Air Force C-5 aircraft. ELC-3 and the Alpha Magnetic Spectrometer are the primary payloads for space shuttle Endeavour's STS-134 mission to the International Space Station. The STS-134 crew will also deliver spare parts including two S-band communications antennas, a high pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields. Endeavour's launch is targeted for July 29, 2010. For information on the STS-134 mission objectives and crew, visit http://www.nasa.gov/shuttle. Photo credit: NASA/Kim Shiflett

Systemic Response to Microgravity: Utilizing GeneLab Datasets to Identify Molecular Targets for Future Hypotheses-Driven Spaceflight Studies

NASA Technical Reports Server (NTRS)

Beheshti, Afshin; Ray, Shayoni; Fogle, Homer; Berrios, Daniel C.; Costes, Sylvain V.

2017-01-01

Biological risks associated with microgravity are a major concern for long-term space travel. Although determination of risk has been a focus for NASA research, data examining systemic (i.e., multi- or pan-tissue) responses to space flight are sparse. To perform our analysis, we utilized the NASA GeneLab database which is a publicly available repository containing a wide array of omics results from experiments conducted with: i) with different flight conditions (space shuttle (STS) missions vs. International Space Station (ISS); ii) a variety of tissues; and 3) assays that measure epigenetic, transcriptional, and protein expression changes. Meta-analysis of the transcriptomic data from 7 different murine and rat data sets, examining tissues such as liver, kidney, adrenal gland, thymus, mammary gland, skin, and skeletal muscle (soleus, extensor digitorum longus, tibialis anterior, quadriceps, and gastrocnemius) revealed for the first time, the existence of potential master regulators coordinating systemic responses to microgravity in rodents. We identified p53, TGF(beta)1 and immune related pathways as the highly prevalent pan-tissue signaling pathways that are affected by microgravity. Some variability in the degree of change in their expression across species, strain and time of flight was also observed. Interestingly, while certain skeletal muscle (gastrocnemius and soleus) exhibited an overall down-regulation of these genes, some other muscle types such as the extensor digitorum longus, tibialis anterior and quadriceps, showed an up-regulated expression, indicative of potential compensatory mechanisms to prevent microgravity-induced atrophy. Key genes isolated by unbiased systems analyses displayed a major overlap between tissue types and flight conditions and established TGF(beta)1 to be the most connected gene across all data sets. Finally, a set of microgravity responsive miRNA signature was identified and based on their predicted functional state and subsequent impact on health, a theoretical health risk score was calculated. The genes and miRNAs identified from our analyses can be targeted for future research involving efficient countermeasure design. Our study thus exemplifies the utility of GeneLab data repository to aid in the process of performing novel hypothesis based spaceflight research aimed at elucidating the global impact of environmental stressors at multiple biological scales.

DNA Fragmentation Factor 45 (DFF45) Gene at 1p36.2 Is Homozygously Deleted and Encodes Variant Transcripts in Neuroblastoma Cell Line1

PubMed Central

Yang, Hong Wei; Chen, Ying Zhang; Piao, Hui Ying; Takita, Junko; Soeda, Eiichi; Hayashi, Yasuhide

2001-01-01

Abstract Recently, loss of heterozygosity (LOH) studies suggest that more than two tumor suppressor genes lie on the short arm of chromosome 1 (1p) in neuroblastoma (NB). To identify candidate tumor suppressor genes in NB, we searched for homozygous deletions in 20 NB cell lines using a high-density STS map spanning chromosome 1p36, a common LOH region in NB. We found that the 45-kDa subunit of the DNA fragmentation factor (DFF45) gene was homozygously deleted in an NB cell line, NB-1. DFF45 is the chaperon of DFF40, and both molecules are necessary for caspase 3 to induce apoptosis. DFF35, a splicing variant of DFF45, is an inhibitor of DFF40. We examined 20 NB cell lines for expression and mutation of DFF45 gene by reverse transcription (RT)-polymerase chain reaction (PCR) and RT-PCR-single-strand conformation polymorphism. Some novel variant transcripts of the DFF45 gene were found in NB cell lines, but not in normal adrenal gland and peripheral blood. These variants may not serve as chaperons of DFF40, but as inhibitors like DFF35, thus disrupting the balance between DFF45 and DFF40. No mutations of the DFF45 gene were found in any NB cell line, suggesting that the DFF45 is not a tumor suppressor gene for NB. However, homozygous deletion of the DFF45 gene in the NB-1 cell line may imply the presence of unknown tumor suppressor genes in this region. PMID:11420752

Prevalence, Virulence Genes, Antimicrobial Susceptibility, and Genetic Diversity of Staphylococcus aureus from Retail Aquatic Products in China

PubMed Central

Rong, Dongli; Wu, Qingping; Xu, Mingfang; Zhang, Jumei; Yu, Shubo

2017-01-01

Staphylococcus aureus is an important food-borne opportunistic pathogen that frequently causes severe blood and tissue infections or even fatal illnesses. Although S. aureus has been extensively studied in livestock and poultry foods in China, limited information has been reported in aquatic products. Accordingly, in this study, we aimed to characterize S. aureus in aquatic products purchased from retail markets in China. In total, 320 aquatic food samples were collected from 32 provincial capitals in China. The results showed that 119 samples (37.2%, 119/320) were positive for S. aureus by both qualitative and quantitative analyses. The contamination levels of 78.2% of samples ranged from 0.3 to 10 MPN/g, and six samples exceeded 110 MPN/g. A total of 119 S. aureus isolates from positive samples were selected to evaluate virulence factors, antibiotic resistance, and molecular characteristics. All S. aureus isolates were evaluated for the presence of 11 virulence genes by multiplex polymerase chain reaction, and α-hemolysin (hlα, 84.9%), fibronectin-binding protein A (fnbA, 79.0%), S. aureus enterotoxin E (see, 53.8%), and Panton-Valentine leucocidin (pvl, 50.4%) were identified as the major genes. These genes formed 56 different profiles, with the major profile identified as pvl-hlα-fnbA (28.6%). The antimicrobial susceptibility of all isolates was analyzed through the disk diffusion method, and the results showed high resistance to β-lactams, macrolides and tetracyclines, but susceptibility to linezolid and vancomycin. In addition, 26 sequence types (STs) were obtained via multilocus sequence typing, including seven novel STs, among which ST1 (20.2%), ST15 (18.5%), and ST188 (13.4%) were the most common STs. All the isolates were mecC negative, but nine isolates carrying mecA were evaluated by staphylococcal cassette chromosome mec (SCCmec) typing, all of which were SCCmecIII or SCCmecIV types. Isolates of SCCmecIII showed a high prevalence and were multidrug resistant. Our results showed that aquatic products could be a vehicle for transmission of virulence genes and multidrug-resistant S. aureus, representing a potential public health risk. The STs identified in this study indicated the genetic diversity of S. aureus, thereby providing important basic data for the dissemination of S. aureus in aquatic products. PMID:28473827

α-ENaC in bullfrog embryo: expression in cement gland, gills and skin.

PubMed

Fujimaki-Aoba, Kayo; Tanaka, Kayoko; Inomata, Reiko; Jensik, Philip J; Takada, Makoto

2014-01-01

The epithelial sodium channel (ENaC) is involved in Na(+) responses such as Na(+) absorption and salt taste. The alpha ENaC subunit (α-ENaC) is expressed in the skin of both the adult and larval (tadpole) bullfrog. α-ENaC expression in the developing bullfrog embryo has not been previously investigated. In this study, the expression of α-ENaC at various stages (Sts.) of bullfrog embryonic development is assessed by western blot and immunofluorescence analysis. Bullfrog α-ENaC (α-fENaC) protein was detected by western blot in embryos at Sts. (Gosner/Shumway) 19, 21 and 25. Immunofluorescence studies indicate that α-fENaC was localized to the embryonic cement glands at St. 18 (muscular response), St. 19 (heart beat) and St. 21 (mouth open and/or cornea transparent), to the external gills at St. 21 and to the outermost cell-layer of the skin at St. 25 (operculum complete). The function(s) of ENaC in these embryonic structures remain to be elucidated.

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-084 (1 June 2011) --- Space shuttle Endeavour approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-080 (1 June 2011) --- Space shuttle Endeavour lands on Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

KSC-2011-4210

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- STS-134 Mission Specialists Mike Fincke, left, chats with STS-134 Assistant Launch Director Pete Nickolenko following the successful return of space shuttle Endeavour to NASA's Kennedy Space Center in Florida. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-066 (1 June 2011) --- Space shuttle Endeavour approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-068 (1 June 2011) --- Space shuttle Endeavour lands on Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Genetic analysis of invasive Escherichia coli in Scotland reveals determinants of healthcare-associated versus community-acquired infections.

PubMed

Goswami, Cosmika; Fox, Stephen; Holden, Matthew; Connor, Martin; Leanord, Alistair; Evans, Thomas J

2018-06-22

Bacteraemia caused by Escherichia coli is a growing problem with a significant mortality. The factors that influence the acquisition and outcome of these infections are not clear. Here, we have linked detailed genetic data from the whole-genome sequencing of 162 bacteraemic isolates collected in Scotland, UK, in 2013-2015, with clinical data in order to delineate bacterial and host factors that influence the acquisition in hospital or the community, outcome and antibiotic resistance. We identified four major sequence types (STs) in these isolates: ST131, ST69, ST73 and ST95. Nearly 50 % of the bacteraemic isolates had a urinary origin. ST69 was genetically distinct from the other STs, with significantly less sharing of accessory genes and with a distinct plasmid population. Virulence genes were widespread and diversely distributed between the dominant STs. ST131 was significantly associated with hospital-associated infections (HAIs), and ST69 with those from the community. However, there was no association of ST with outcome, although patients with HAI had a higher immediate mortality compared to those with community-associated infections (CAIs). Genome-wide association studies revealed genes involved in antibiotic persistence as significantly associated with HAIs and those encoding elements of a type VI secretion system with CAIs. Antibiotic resistance was common, and there were networks of correlated resistance genes and phenotypic antibiotic resistance. This study has revealed the complex interactions between the genotype of E. coli and its ability to cause bacteraemia, and some of the determinants influencing hospital or community acquisition. In part, these are shaped by antibiotic usage, but strain-specific factors are also important.

Space Shuttle Atlantis Landing / STS-129 Mission

NASA Image and Video Library

2009-11-27

PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - A fire and rescue truck is in place beside Runway 33 if needed to support the landing of space shuttle Atlantis at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. After 11 days in space, Atlantis completed the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jack Pfaller

Analysis of Multilocus Sequence Typing and Virulence Characterization of Listeria monocytogenes Isolates from Chinese Retail Ready-to-Eat Food

PubMed Central

Wu, Shi; Wu, Qingping; Zhang, Jumei; Chen, Moutong; Guo, Weipeng

2016-01-01

Eighty Listeria monocytogenes isolates were obtained from Chinese retail ready-to-eat (RTE) food and were previously characterized with serotyping and antibiotic susceptibility tests. The aim of this study was to characterize the subtype and virulence potential of these L. monocytogenes isolates by multilocus sequence typing (MLST), virulence-associate genes, epidemic clones (ECs), and sequence analysis of the important virulence factor: internalin A (inlA). The result of MLST revealed that these L. monocytogenes isolates belonged to 14 different sequence types (STs). With the exception of four new STs (ST804, ST805, ST806, and ST807), all other STs observed in this study have been associated with human listeriosis and outbreaks to varying extents. Six virulence-associate genes (inlA, inlB, inlC, inlJ, hly, and llsX) were selected and their presence was investigated using PCR. All strains carried inlA, inlB, inlC, inlJ, and hly, whereas 38.8% (31/80) of strains harbored the listeriolysin S genes (llsX). A multiplex PCR assay was used to evaluate the presence of markers specific to epidemic clones of L. monocytogenes and identified 26.3% (21/80) of ECI in the 4b-4d-4e strains. Further study of inlA sequencing revealed that most strains contained the full-length InlA required for host cell invasion, whereas three mutations lead to premature stop codons (PMSC) within a novel PMSCs at position 326 (GAA → TAA). MLST and inlA sequence analysis results were concordant, and different virulence potentials within isolates were observed. These findings suggest that L. monocytogenes isolates from RTE food in China could be virulent and be capable of causing human illness. Furthermore, the STs and virulence profiles of L. monocytogenes isolates have significant implications for epidemiological and public health studies of this pathogen. PMID:26909076

Analysis of Multilocus Sequence Typing and Virulence Characterization of Listeria monocytogenes Isolates from Chinese Retail Ready-to-Eat Food.

PubMed

Wu, Shi; Wu, Qingping; Zhang, Jumei; Chen, Moutong; Guo, Weipeng

2016-01-01

Eighty Listeria monocytogenes isolates were obtained from Chinese retail ready-to-eat (RTE) food and were previously characterized with serotyping and antibiotic susceptibility tests. The aim of this study was to characterize the subtype and virulence potential of these L. monocytogenes isolates by multilocus sequence typing (MLST), virulence-associate genes, epidemic clones (ECs), and sequence analysis of the important virulence factor: internalin A (inlA). The result of MLST revealed that these L. monocytogenes isolates belonged to 14 different sequence types (STs). With the exception of four new STs (ST804, ST805, ST806, and ST807), all other STs observed in this study have been associated with human listeriosis and outbreaks to varying extents. Six virulence-associate genes (inlA, inlB, inlC, inlJ, hly, and llsX) were selected and their presence was investigated using PCR. All strains carried inlA, inlB, inlC, inlJ, and hly, whereas 38.8% (31/80) of strains harbored the listeriolysin S genes (llsX). A multiplex PCR assay was used to evaluate the presence of markers specific to epidemic clones of L. monocytogenes and identified 26.3% (21/80) of ECI in the 4b-4d-4e strains. Further study of inlA sequencing revealed that most strains contained the full-length InlA required for host cell invasion, whereas three mutations lead to premature stop codons (PMSC) within a novel PMSCs at position 326 (GAA → TAA). MLST and inlA sequence analysis results were concordant, and different virulence potentials within isolates were observed. These findings suggest that L. monocytogenes isolates from RTE food in China could be virulent and be capable of causing human illness. Furthermore, the STs and virulence profiles of L. monocytogenes isolates have significant implications for epidemiological and public health studies of this pathogen.

Evaluation of in vitro macrophage differentiation during space flight

NASA Astrophysics Data System (ADS)

Ortega, M. Teresa; Lu, Nanyan; Chapes, Stephen K.

2012-05-01

We differentiated mouse bone marrow cells in the presence of recombinant macrophage colony stimulating (rM-CSF) factor for 14 days during the flight of space shuttle Space Transportation System (STS)-126. We tested the hypothesis that the receptor expression for M-CSF, c-Fms was reduced. We used flow cytometry to assess molecules on cells that were preserved during flight to define the differentiation state of the developing bone marrow macrophages; including CD11b, CD31, CD44, Ly6C, Ly6G, F4/80, Mac2, c-Fos as well as c-Fms. In addition, RNA was preserved during the flight and was used to perform a gene microarray. We found that there were significant differences in the number of macrophages that developed in space compared to controls maintained on Earth. We found that there were significant changes in the distribution of cells that expressed CD11b, CD31, F4/80, Mac2, Ly6C and c-Fos. However, there were no changes in c-Fms expression and no consistent pattern of advanced or retarded differentiation during space flight. We also found a pattern of transcript levels that would be consistent with a relatively normal differentiation outcome but increased proliferation by the bone marrow macrophages that were assayed after 14 days of space flight. There also was a surprising pattern of space flight influence on genes of the coagulation pathway. These data confirm that a space flight can have an impact on the in vitro development of macrophages from mouse bone marrow cells.

Evaluation of in vitro macrophage differentiation during space flight.

PubMed

Ortega, M Teresa; Lu, Nanyan; Chapes, Stephen K

2012-05-15

We differentiated mouse bone marrow cells in the presence of recombinant macrophage colony stimulating (rM-CSF) factor for 14 days during the flight of space shuttle Space Transportation System (STS)-126. We tested the hypothesis that the receptor expression for M-CSF, c-Fms was reduced. We used flow cytometry to assess molecules on cells that were preserved during flight to define the differentiation state of the developing bone marrow macrophages; including CD11b, CD31, CD44, Ly6C, Ly6G, F4/80, Mac2, c-Fos as well as c-Fms. In addition, RNA was preserved during the flight and was used to perform a gene microarray. We found that there were significant differences in the number of macrophages that developed in space compared to controls maintained on Earth. We found that there were significant changes in the distribution of cells that expressed CD11b, CD31, F4/80, Mac2, Ly6C and c-Fos. However, there were no changes in c-Fms expression and no consistent pattern of advanced or retarded differentiation during space flight. We also found a pattern of transcript levels that would be consistent with a relatively normal differentiation outcome but increased proliferation by the bone marrow macrophages that were assayed after 14 days of space flight. There also was a surprising pattern of space flight influence on genes of the coagulation pathway. These data confirm that a space flight can have an impact on the in vitro development of macrophages from mouse bone marrow cells.

Targeting Selectins and Their Ligands in Cancer.

PubMed

Natoni, Alessandro; Macauley, Matthew S; O'Dwyer, Michael E

2016-01-01

Aberrant glycosylation is a hallmark of cancer cells with increased evidence pointing to a role in tumor progression. In particular, aberrant sialylation of glycoproteins and glycolipids has been linked to increased immune cell evasion, drug evasion, drug resistance, tumor invasiveness, and vascular dissemination, leading to metastases. Hypersialylation of cancer cells is largely the result of overexpression of sialyltransferases (STs). Differentially, humans express twenty different STs in a tissue-specific manner, each of which catalyzes the attachment of sialic acids via different glycosidic linkages (α2-3, α2-6, or α2-8) to the underlying glycan chain. One important mechanism whereby overexpression of STs contributes to an enhanced metastatic phenotype is via the generation of selectin ligands. Selectin ligand function requires the expression of sialyl-Lewis X and its structural isomer sialyl-Lewis A, which are synthesized by the combined action of alpha α1-3-fucosyltransferases, α2-3-sialyltransferases, β1-4-galactosyltranferases, and N-acetyl-β-glucosaminyltransferases. The α2-3-sialyltransferases ST3Gal4 and ST3Gal6 are critical to the generation of functional E- and P-selectin ligands and overexpression of these STs have been linked to increased risk of metastatic disease in solid tumors and poor outcome in multiple myeloma. Thus, targeting selectins and their ligands as well as the enzymes involved in their generation, in particular STs, could be beneficial to many cancer patients. Potential strategies include ST inhibition and the use of selectin antagonists, such as glycomimetic drugs and antibodies. Here, we review ongoing efforts to optimize the potency and selectivity of ST inhibitors, including the potential for targeted delivery approaches, as well as evaluate the potential utility of selectin inhibitors, which are now in early clinical development.

Targeting Selectins and Their Ligands in Cancer

PubMed Central

Natoni, Alessandro; Macauley, Matthew S.; O’Dwyer, Michael E.

2016-01-01

Aberrant glycosylation is a hallmark of cancer cells with increased evidence pointing to a role in tumor progression. In particular, aberrant sialylation of glycoproteins and glycolipids has been linked to increased immune cell evasion, drug evasion, drug resistance, tumor invasiveness, and vascular dissemination, leading to metastases. Hypersialylation of cancer cells is largely the result of overexpression of sialyltransferases (STs). Differentially, humans express twenty different STs in a tissue-specific manner, each of which catalyzes the attachment of sialic acids via different glycosidic linkages (α2-3, α2-6, or α2-8) to the underlying glycan chain. One important mechanism whereby overexpression of STs contributes to an enhanced metastatic phenotype is via the generation of selectin ligands. Selectin ligand function requires the expression of sialyl-Lewis X and its structural isomer sialyl-Lewis A, which are synthesized by the combined action of alpha α1-3-fucosyltransferases, α2-3-sialyltransferases, β1-4-galactosyltranferases, and N-acetyl-β-glucosaminyltransferases. The α2-3-sialyltransferases ST3Gal4 and ST3Gal6 are critical to the generation of functional E- and P-selectin ligands and overexpression of these STs have been linked to increased risk of metastatic disease in solid tumors and poor outcome in multiple myeloma. Thus, targeting selectins and their ligands as well as the enzymes involved in their generation, in particular STs, could be beneficial to many cancer patients. Potential strategies include ST inhibition and the use of selectin antagonists, such as glycomimetic drugs and antibodies. Here, we review ongoing efforts to optimize the potency and selectivity of ST inhibitors, including the potential for targeted delivery approaches, as well as evaluate the potential utility of selectin inhibitors, which are now in early clinical development. PMID:27148485

KSC-2011-1797

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, media photograph space shuttle Endeavour's move, or "rollover," to the Vehicle Assembly Building (VAB) from Orbiter Processing Facility-2. In the VAB, Endeavour will be lifted into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final and upcoming STS-134 mission. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

KSC-2011-1922

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- In the transfer aisle of the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers attach an overhead crane to space shuttle Endeavour. The crane will lift the spacecraft into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final mission, STS-134. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-1923

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- In the transfer aisle of the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, a worker attaches an overhead crane to space shuttle Endeavour. The crane will lift the spacecraft into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final mission, STS-134. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-1902

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, space shuttle Endeavour approaches the Vehicle Assembly Building, or VAB, on its move from Orbiter Processing Facility-2 where it was processed for its final mission, STS-134. In the VAB, Endeavour will be lifted into a high bay where it will be joined to its external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1927

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- In the transfer aisle of the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, a worker attaches an overhead crane to space shuttle Endeavour. The crane will lift the spacecraft into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final mission, STS-134. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-1903

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, space shuttle Endeavour approaches the Vehicle Assembly Building, or VAB, on its move from Orbiter Processing Facility-2 where it was processed for its final mission, STS-134. In the VAB, Endeavour will be lifted into a high bay where it will be joined to its external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1926

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- In the transfer aisle of the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers attach an overhead crane to the rear of space shuttle Endeavour. The crane will lift the spacecraft into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final mission, STS-134. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-3584

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. - Under a vividly painted blue sky, space shuttle Endeavour awaits liftoff on Launch Pad 39A at NASA's Kennedy Space Center in Florida. STS-134 will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. May 16 at 8:56 a.m. will be the second launch attempt for Endeavour. The first attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

Prevalence of Secondary Traumatic Stress Among Psychiatric Nurses in Greece.

PubMed

Mangoulia, Polyxeni; Koukia, Evmorfia; Alevizopoulos, George; Fildissis, George; Katostaras, Theofanis

2015-10-01

The aim of this study was to investigate the prevalence of secondary traumatic stress/compassion fatigue (STS/CF), burnout (BO) and compassion satisfaction (CS) in psychiatric nurses, and their risk factors. The Professional Quality of Life Scale (ProQOL R-IV) and a demographic and work related characteristics questionnaire were distributed to 174 psychiatric nurses in 12 public hospitals in Greece. The majority of participants were at the high risk category for STS/CF (44.8%) and BO (49.4%), while only 8.1% of nurses expressed high potential for CS. Awareness of the factors associated with STS may help nurses to prevent or offset the development of this condition. Copyright © 2015 Elsevier Inc. All rights reserved.

Changes in mouse thymus and spleen after return from the STS-135 mission in space.

PubMed

Gridley, Daila S; Mao, Xiao Wen; Stodieck, Louis S; Ferguson, Virginia L; Bateman, Ted A; Moldovan, Maria; Cunningham, Christopher E; Jones, Tamako A; Slater, Jerry M; Pecaut, Michael J

2013-01-01

Our previous results with flight (FLT) mice showed abnormalities in thymuses and spleens that have potential to compromise immune defense mechanisms. In this study, the organs were further evaluated in C57BL/6 mice after Space Shuttle Atlantis returned from a 13-day mission. Thymuses and spleens were harvested from FLT mice and ground controls housed in similar animal enclosure modules (AEM). Organ and body mass, DNA fragmentation and expression of genes related to T cells and cancer were determined. Although significance was not obtained for thymus mass, DNA fragmentation was greater in the FLT group (P<0.01). Spleen mass alone and relative to body mass was significantly decreased in FLT mice (P<0.05). In FLT thymuses, 6/84 T cell-related genes were affected versus the AEM control group (P<0.05; up: IL10, Il18bp, Il18r1, Spp1; down: Ccl7, IL6); 15/84 cancer-related genes had altered expression (P<0.05; up: Casp8, FGFR2, Figf, Hgf, IGF1, Itga4, Ncam1, Pdgfa, Pik3r1, Serpinb2, Sykb; down: Cdc25a, E2F1, Mmp9, Myc). In the spleen, 8/84 cancer-related genes were affected in FLT mice compared to AEM controls (P<0.05; up: Cdkn2a; down: Birc5, Casp8, Ctnnb1, Map2k1, Mdm2, NFkB1, Pdgfa). Pathway analysis (apoptosis signaling and checkpoint regulation) was used to map relationships among the cancer-related genes. The results showed that a relatively short mission in space had a significant impact on both organs. The findings also indicate that immune system aberrations due to stressors associated with space travel should be included when estimating risk for pathologies such as cancer and infection and in designing appropriate countermeasures. Although this was the historic last flight of NASA's Space Shuttle Program, exploration of space will undoubtedly continue.

Changes in Mouse Thymus and Spleen after Return from the STS-135 Mission in Space

PubMed Central

Gridley, Daila S.; Mao, Xiao Wen; Stodieck, Louis S.; Ferguson, Virginia L.; Bateman, Ted A.; Moldovan, Maria; Cunningham, Christopher E.; Jones, Tamako A.; Slater, Jerry M.; Pecaut, Michael J.

2013-01-01

Our previous results with flight (FLT) mice showed abnormalities in thymuses and spleens that have potential to compromise immune defense mechanisms. In this study, the organs were further evaluated in C57BL/6 mice after Space Shuttle Atlantis returned from a 13-day mission. Thymuses and spleens were harvested from FLT mice and ground controls housed in similar animal enclosure modules (AEM). Organ and body mass, DNA fragmentation and expression of genes related to T cells and cancer were determined. Although significance was not obtained for thymus mass, DNA fragmentation was greater in the FLT group (P<0.01). Spleen mass alone and relative to body mass was significantly decreased in FLT mice (P<0.05). In FLT thymuses, 6/84 T cell-related genes were affected versus the AEM control group (P<0.05; up: IL10, Il18bp, Il18r1, Spp1; down: Ccl7, IL6); 15/84 cancer-related genes had altered expression (P<0.05; up: Casp8, FGFR2, Figf, Hgf, IGF1, Itga4, Ncam1, Pdgfa, Pik3r1, Serpinb2, Sykb; down: Cdc25a, E2F1, Mmp9, Myc). In the spleen, 8/84 cancer-related genes were affected in FLT mice compared to AEM controls (P<0.05; up: Cdkn2a; down: Birc5, Casp8, Ctnnb1, Map2k1, Mdm2, NFkB1, Pdgfa). Pathway analysis (apoptosis signaling and checkpoint regulation) was used to map relationships among the cancer–related genes. The results showed that a relatively short mission in space had a significant impact on both organs. The findings also indicate that immune system aberrations due to stressors associated with space travel should be included when estimating risk for pathologies such as cancer and infection and in designing appropriate countermeasures. Although this was the historic last flight of NASA’s Space Shuttle Program, exploration of space will undoubtedly continue. PMID:24069384

DNA methylation profiles of long- and short-term glioblastoma survivors

PubMed Central

Shinawi, Thoraia; Hill, Victoria K.; Krex, Dietmar; Schackert, Gabriele; Gentle, Dean; Morris, Mark R.; Wei, Wenbin; Cruickshank, Garth; Maher, Eamonn R.; Latif, Farida

2013-01-01

Glioblastoma (GBM) is the most common and malignant type of primary brain tumor in adults and prognosis of most GBM patients is poor. However, a small percentage of patients show a long term survival of 36 mo or longer after diagnosis. Epigenetic profiles can provide molecular markers for patient prognosis: recently, a G-CIMP positive phenotype associated with IDH1 mutations has been described for GBMs with good prognosis. In the present analysis we performed genome-wide DNA methylation profiling of short-term survivors (STS; overall survival < 1 y) and long-term survivors (LTS; overall survival > 3 y) by utilizing the HumanMethylation450K BeadChips to assess quantitative methylation at > 480,000 CpG sites. Cluster analysis has shown that a subset of LTS showed a G-CIMP positive phenotype that was tightly associated with IDH1 mutation status and was confirmed by analysis of the G-CIMP signature genes. Using high stringency criteria for differential hypermethylation between non-cancer brain and tumor samples, we identified 2,638 hypermethylated CpG loci (890 genes) in STS GBMs, 3,101 hypermethylated CpG loci (1,062 genes) in LTS (wild type IDH1) and 11,293 hypermethylated CpG loci in LTS (mutated for IDH1), reflecting the CIMP positive phenotype. The location of differentially hypermethylated CpG loci with respect to CpG content, neighborhood context and functional genomic distribution was similar in our sample set, with the majority of CpG loci residing in CpG islands and in gene promoters. Our preliminary study also identified a set of CpG loci differentially hypermethylated between STS and LTS cases, including members of the homeobox gene family (HOXD8, HOXD13 and HOXC4), the transcription factors NR2F2 and TFAP2A, and Dickkopf 2, a negative regulator of the wnt/β-catenin signaling pathway. PMID:23291739

KSC-2009-4202

NASA Image and Video Library

2009-07-16

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians keep watch as the control moment gyroscope is lowered toward an EXPRESS Logistics Carrier. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12 . Photo credit: NASA/Jack Pfaller

KSC-2009-4200

NASA Image and Video Library

2009-07-16

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians keep watch as the control moment gyroscope is moved toward an EXPRESS Logistics Carrier. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12 . Photo credit: NASA/Jack Pfaller

KSC-2009-4714

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, the nitrogen tank assembly is moved toward the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4716

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, the nitrogen tank assembly is lowered onto the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4685

NASA Image and Video Library

2009-08-14

CAPE CANAVERAL, Fla. – In NASA Kennedy Space Center's Space Station Processing Facility, an overhead crane moves the Express Logistics Carrier, or ELC, to a rotation stand. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Kim Shiflett

KSC-2009-4715

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, the nitrogen tank assembly is lowered toward the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-2245

NASA Image and Video Library

2009-03-21

CAPE CANAVERAL, Fla. – Inside the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians check the EXPRESS Logistics Carrier for the STS-129 mission after its cover was removed. The carrier is part of the payload on space shuttle Atlantis, which will deliver to the International Space Station components including two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Tim Jacobs

KSC-2009-2244

NASA Image and Video Library

2009-03-21

CAPE CANAVERAL, Fla. – Inside the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians remove the cover from around the EXPRESS Logistics Carrier for the STS-129 mission. The carrier is part of the payload on space shuttle Atlantis, which will deliver to the International Space Station components including two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Tim Jacobs

KSC-2009-2242

NASA Image and Video Library

2009-03-21

CAPE CANAVERAL, Fla. – The truck carrying the EXPRESS Logistics Carrier for the STS-129 mission arrives at the Space Station Processing Facility at NASA's Kennedy Space Center in Florida. The carrier is part of the payload on space shuttle Atlantis, which will deliver to the International Space Station components including two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Tim Jacobs

PUMA promotes Bax translocation in FOXO3a-dependent pathway during STS-induced apoptosis

NASA Astrophysics Data System (ADS)

Zhang, Yingjie; Chen, Qun

2009-08-01

PUMA (p53 up-regulated modulator of apoptosis, also called Bbc3) was first identified as a BH3-only Bcl-2 family protein that is transcriptionally up-regulated by p53 and activated upon p53-dependent apoptotic stimuli, such as treatment with DNA-damaging drugs or UV irradiation. Recently studies have been shown that Puma is also up-regulated in response to certain p53-independent apoptotic stimuli, such as growth factor deprivation or treatment with glucocorticoids or STS (staurosporine). However, the molecular mechanisms of PUMA up-regulation and how PUMA functions in response to p53-independent apoptotic stimuli remain poorly understood. In this study, based on real-time single cell analysis, flow cytometry and western blotting technique, we investigated the function of PUMA in living human lung adenocarcinoma cells (ASTC-a-1) after STS treatment. Our results show that FOXO3a was activated by STS stimulation and then translocated from cytosol to nucleus. The expression of PUMA was up-regulated via a FOXO3a-dependent manner after STS treatment, while p53 had little function in this process. Moreover, cell apoptosis and Bax translocation induced by STS were not blocked by Pifithrin-α (p53 inhibitor), which suggested that p53 was not involved in this signaling pathway. Taken together, these results indicate that PUMA promoted Bax translocation in a FOXO3a-dependment pathway during STS-induced apoptosis, while p53 was dispensable in this process.

Sdt97: A Point Mutation in the 5′ Untranslated Region Confers Semidwarfism in Rice

PubMed Central

Tong, Jiping; Han, Zhengshu; Han, Aonan; Liu, Xuejun; Zhang, Shiyong; Fu, Binying; Hu, Jun; Su, Jingping; Li, Shaoqing; Wang, Shengjun; Zhu, Yingguo

2016-01-01

Semidwarfism is an important agronomic trait in rice breeding programs. The semidwarf mutant gene Sdt97 was previously described. However, the molecular mechanism underlying the mutant is yet to be elucidated. In this study, we identified the mutant gene by a map-based cloning method. Using a residual heterozygous line (RHL) population, Sdt97 was mapped to the long arm of chromosome 6 in the interval of nearly 60 kb between STS marker N6 and SNP marker N16 within the PAC clone P0453H04. Sequencing of the candidate genes in the target region revealed that a base transversion from G to C occurred in the 5′ untranslated region of Sdt97. qRT-PCR results confirmed that the transversion induced an obvious change in the expression pattern of Sdt97 at different growth and developmental stages. Plants transgenic for Sdt97 resulted in the restoration of semidwarfism of the mutant phenotype, or displayed a greater dwarf phenotype than the mutant. Our results indicate that a point mutation in the 5′ untranslated region of Sdt97 confers semidwarfism in rice. Functional analysis of Sdt97 will open a new field of study for rice semidwarfism, and also expand our knowledge of the molecular mechanism of semidwarfism in rice. PMID:27172200

A high-resolution whole genome radiation hybrid map of human chromosome 17q22-q25.3 across the genes for GH and TK

DOE Office of Scientific and Technical Information (OSTI.GOV)

Foster, J.W.; Schafer, A.J.; Critcher, R.

1996-04-15

We have constructed a whole genome radiation hybrid (WG-RH) map across a region of human chromosome 17q, from growth hormone (GH) to thymidine kinase (TK). A panel of 128 WG-RH hybrid cell lines generated by X-irradiation and fusion has been tested for the retention of 39 sequence-tagged site (STS) markers by the polymerase chain reaction. This genome mapping technique has allowed the integration of existing VNTR and microsatellite markers with additional new markers and existing STS markers previously mapped to this region by other means. The WG-RH map includes eight expressed sequence tag (EST) and three anonymous markers developed formore » this study, together with 23 anonymous microsatellites and five existing ESTs. Analysis of these data resulted in a high-density comprehensive map across this region of the genome. A subset of these markers has been used to produce a framework map consisting of 20 loci ordered with odds greater than 1000:1. The markers are of sufficient density to build a YAC contig across this region based on marker content. We have developed sequence tags for both ends of a 2.1-Mb YAC and mapped these using the WG-RH panel, allowing a direct comparison of cRay{sub 6000} to physical distance. 31 refs., 3 figs., 2 tabs.« less

Occurrence of plasmid-mediated quinolone resistance genes in Escherichia coli and Klebsiella spp. recovered from Corvus brachyrhynchos and Corvus corax roosting in Canada.

PubMed

Janecko, Nicol; Halova, Dana; Jamborova, Ivana; Papousek, Ivo; Masarikova, Martina; Dolejska, Monika; Literak, Ivan

2018-04-19

The spread of antimicrobial resistance from human activity derived sources to natural habitats implicates wildlife as potential vectors of antimicrobial resistance transfer. Wild birds, including corvid species can disseminate mobile genetic resistance determinants through feces. This study aimed to determine the occurrence of plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli and Klebsiella spp. isolates obtained from winter roosting sites of American crows (Corvus brachyrhynchos) and common ravens (Corvus corax) in Canada. Fecal swabs were collected at five roosting sites across Canada. Selective media isolation and multiplex PCR screening was utilized to identify PMQR genes followed by gene sequencing, PFGE and MLST to characterize isolates. Despite the low prevalence of E. coli containing PMQR (1.3%, 6/449), qnrS1, qnrB19, qnrC, oqxAB and aac(6')-Ib-cr genes were found in five sequence types (ST), including E. coli ST 131. Conversely, one isolate of Klebsiella pneumoniae contained the plasmid-mediated resistance gene qnrB19. Five different K. pneumoniae STs were identified, including two novel types. The occurrence of PMQR genes and STs of public health significance in E. coli and Klebsiella pneumoniae recovered from corvids gives further evidence of the anthropogenic derived dissemination of antimicrobial resistance determinants at the human activity-wildlife-environment interface. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Becerikli, Mustafa; Jacobsen, Frank; Rittig, Andrea

Soft tissue sarcomas (STS) are characterized by co-participation of several epigenetic and genetic events during tumorigenesis. Having bypassed cellular senescence barriers during oncogenic transformation, the factors further affecting growth rate of STS cells remain poorly understood. Therefore, we investigated the role of gene silencing (DNA promoter methylation of LINE-1, PTEN), genetic aberrations (karyotype, KRAS and BRAF mutations) as well as their contribution to the proliferation rate and migratory potential that underlies “initial” and “final” passage sarcoma cells. Three different cell lines were used, SW982 (synovial sarcoma), U2197 (malignant fibrous histiocytoma (MFH)) and HT1080 (fibrosarcoma). Increased proliferative potential of final passagemore » STS cells was not associated with significant differences in methylation (LINE-1, PTEN) and mutation status (KRAS, BRAF), but it was dependent on the amount of chromosomal aberrations. Collectively, our data demonstrate that these fairly differentiated/advanced cancer cell lines have still the potential to gain an additional spontaneous growth benefit without external influences and that maintenance of increased proliferative potential towards longevity of STS cells (having crossed senescence barriers) may be independent of overt epigenetic alterations. -- Highlights: Increased proliferative potential of late passage STS cells was: • Not associated with epigenetic changes (methylation changes at LINE-1, PTEN). • Not associated with mutation status of KRAS, BRAF. • Dependent on presence/absence of chromosomal aberrations.« less

KSC-2009-3614

NASA Image and Video Library

2009-06-08

CAPE CANAVERAL, Fla. – During a media event at NASA's Kennedy Space Center in Florida to showcase the newest section of the International Space Station, the Tranquility node, STS-130 Commander George Zamka speaks to the media and guests. Tranquility will be delivered to the station during space shuttle Endeavour's STS-130 mission, targeted for launch in February 2010. Others present at right of Zamka are Russ Romanella, director of the ISS and Payload Processing Directorate, STS-130 Pilot Terry Virts and Mission Specialists Stephen Robinson and Kathryn Hire, Philippe Deloo, ISS Nodes project manager with the European Space Agency, and Rafael Garcia, ISS Nodes and Express Logistics Carrier project manager with NASA's Johnson Space Center. Managers from NASA, the European Space Agency, Thales Alenia Space and Boeing -- the organizations involved in building and processing the module for flight -- were available for a question-and-answer session during the event. Tranquility will be delivered to the station during space shuttle Endeavour's STS-130 mission, targeted for launch in February 2010. Photo credit: NASA/Jim Grossmann

Cultural adaptation in measuring common client characteristics with an urban Mainland Chinese sample.

PubMed

Song, Xiaoxia; Anderson, Timothy; Beutler, Larry E; Sun, Shijin; Wu, Guohong; Kimpara, Satoko

2015-01-01

This study aimed to develop a culturally adapted version of the Systematic Treatment Selection-Innerlife (STS) in China. A total of 300 nonclinical participants collected from Mainland China and 240 nonclinical US participants were drawn from archival data. A Chinese version of the STS was developed, using translation and back-translation procedures. After confirmatory factor analysis (CFA) of the original STS sub scales failed on both samples, exploratory factor analysis (EFA) was then used to access whether a simple structure would emerge on these STS treatment items. Parallel analysis and minimum average partial were used to determine the number of factor to retain. Three cross-cultural factors were found in this study, Internalized Distress, Externalized Distress and interpersonal relations. This supported that regardless of whether one is in presumably different cultural contexts of the USA or China, psychological distress is expressed in a few basic channels of internalized distress, externalized distress, and interpersonal relations, from which different manifestations in different culture were also discussed.

KSC-2011-4206

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- NASA Administrator Charlie Bolden, left, and NASA Kennedy Space Center Director Bob Cabana greet space shuttle Endeavour's STS-134 commander and pilot following their successful trip home. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4207

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Shuttle Launch Director Mike Leinbach, left, greets space shuttle Endeavour's STS-134 crew members following their successful trip home. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4208

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour's STS-134 crew members talk with employees following their successful trip home. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-2509

NASA Image and Video Library

2011-03-29

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour's STS-134 crew members pose for a group photo on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. While at Kennedy, Endeavour's crew will participate in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training in preparation for the upcoming STS-134 mission. From left, are Mission Specialists Greg Chamitoff, European Space Agency astronaut Roberto Vittori, Andrew Feustel, Commander Mark Kelly, Pilot Greg H. Johnson, and Mission Specialist Michael Fincke. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-2519

NASA Image and Video Library

2011-03-29

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour's STS-134 crew members pose for a group photo on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. While at Kennedy, Endeavour's crew will participate in a launch countdown dress rehearsal called the Terminal Countdown Demonstration Test (TCDT) and related training in preparation for the upcoming STS-134 mission. From left, are Mission Specialists Greg Chamitoff, European Space Agency astronaut Roberto Vittori, Andrew Feustel, Commander Mark Kelly, Pilot Greg H. Johnson, and Mission Specialist Michael Fincke. Endeavour and its six STS-134 crew members will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. This will be the final spaceflight for Endeavour. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

Gamma-band activity in the human superior temporal sulcus during mentalizing from nonverbal social cues.

PubMed

Cohen, Michael X; David, Nicole; Vogeley, Kai; Elger, Christian E

2009-01-01

The posterior superior temporal sulcus (pSTS) is a key structure for our ability to infer others' mental states based on social cues including facial expressions, body posture, and gestures ("mentalizing"), but the neural mechanisms of this ability remain largely unknown. We recorded electrocorticogram directly from the pSTS in humans to show that enhanced neural oscillations in the gamma frequency range (35-55 Hz) accompany mentalizing. One patient with a lesion in pSTS was tested behaviorally on this task; he was unable to infer a virtual character's preferences from nonverbal social cues. Enhanced coherent gamma oscillations in the patients with intact pSTS may reflect a process by which social signals are bound into a unified representation to support mentalizing. This may be relevant for other social cognitive processes, as well as to the study of autism spectrum disorders, for which both mentalizing deficits and abnormal gamma activity have been reported.

Structural and kinetic analysis of the unnatural fusion protein 4-coumaroyl-CoA ligase::stilbene synthase

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Yechun; Yi, Hankuil; Wang, Melissa

2012-10-24

To increase the biochemical efficiency of biosynthetic systems, metabolic engineers have explored different approaches for organizing enzymes, including the generation of unnatural fusion proteins. Previous work aimed at improving the biosynthesis of resveratrol, a stilbene associated a range of health-promoting activities, in yeast used an unnatural engineered fusion protein of Arabidopsis thaliana (thale cress) 4-coumaroyl-CoA ligase (At4CL1) and Vitis vinifera (grape) stilbene synthase (VvSTS) to increase resveratrol levels 15-fold relative to yeast expressing the individual enzymes. Here we present the crystallographic and biochemical analysis of the 4CL::STS fusion protein. Determination of the X-ray crystal structure of 4CL::STS provides the firstmore » molecular view of an artificial didomain adenylation/ketosynthase fusion protein. Comparison of the steady-state kinetic properties of At4CL1, VvSTS, and 4CL::STS demonstrates that the fusion protein improves catalytic efficiency of either reaction less than 3-fold. Structural and kinetic analysis suggests that colocalization of the two enzyme active sites within 70 {angstrom} of each other provides the basis for enhanced in vivo synthesis of resveratrol.« less

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-077 (1 June 2011) --- Xenon lights help lead space shuttle Endeavour home to NASA's Kennedy Space Center in Florida. Endeavour landed for the final time on the Shuttle Landing Facility's Runway 15, marking the 24th night landing of NASA's Space Shuttle Program. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-03

STS134-S-111 (1 June 2011) --- Space shuttle Endeavour approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time marking the 24th night landing of NASA's Space Shuttle Program. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-03

STS134-S-112 (1 June 2011) --- Space shuttle Endeavour touches down on Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time marking the 24th night landing of NASA's Space Shuttle Program. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-03

STS134-S-113 (1 June 2011) --- Xenon lights help lead space shuttle Endeavour home to NASA's Kennedy Space Center in Florida. Endeavour landed for the final time on the Shuttle Landing Facility's Runway 15, marking the 24th night landing of NASA's Space Shuttle Program. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-089 (1 June 2011) --- Xenon lights help lead space shuttle Endeavour home to NASA's Kennedy Space Center in Florida. Endeavour landed for the final time on the Shuttle Landing Facility's Runway 15, marking the 24th night landing of NASA's Space Shuttle Program. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-072 (1 June 2011) --- Xenon lights illuminate space shuttle Endeavour's unfurled drag chute as the vehicle rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-070 (1 June 2011) --- Xenon lights illuminate space shuttle Endeavour's unfurled drag chute as the vehicle rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-03

STS134-S-115 (1 June 2011) --- Xenon lights help lead space shuttle Endeavour home to NASA's Kennedy Space Center in Florida. Endeavour landed for the final time on the Shuttle Landing Facility's Runway 15, marking the 24th night landing of NASA's Space Shuttle Program. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-073 (1 June 2011) --- Xenon lights illuminate space shuttle Endeavour's unfurled drag chute as the vehicle rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-069 (1 June 2011) --- Xenon lights illuminate space shuttle Endeavour's unfurled drag chute as the vehicle rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-079 (1 June 2011) --- Xenon lights help lead space shuttle Endeavour home to NASA's Kennedy Space Center in Florida. Endeavour landed for the final time on the Shuttle Landing Facility's Runway 15, marking the 24th night landing of NASA's Space Shuttle Program. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

KSC-2009-6303

NASA Image and Video Library

2009-11-15

CAPE CANAVERAL, Fla. - After nightfall on Launch Pad 39A at NASA's Kennedy Space Center in Florida, xenon lights reveal space shuttle Atlantis awaiting launch on its mobile launcher platform. Liftoff of the STS-129 mission is set for 2:28 p.m. EST Nov. 16. The pad's rotating service structure, at left, which provides weather protection and access for technicians to work on the shuttle, began being retracted at 5:20 p.m. EST and was in the park position by 5:56 p.m. On STS-129, the crew will deliver to the International Space Station two Express Logistics Carriers, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Troy Cryder

KSC-2009-6304

NASA Image and Video Library

2009-11-15

CAPE CANAVERAL, Fla. - After nightfall on Launch Pad 39A at NASA's Kennedy Space Center in Florida, xenon lights reveal space shuttle Atlantis awaiting launch on its mobile launcher platform. Liftoff of the STS-129 mission is set for 2:28 p.m. EST Nov. 16. The pad's rotating service structure, at left, which provides weather protection and access for technicians to work on the shuttle, began being retracted at 5:20 p.m. EST and was in the park position by 5:56 p.m. On STS-129, the crew will deliver to the International Space Station two Express Logistics Carriers, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Troy Cryder

Insertion sequence ISRP10 inactivation of the oprD gene in imipenem-resistant Pseudomonas aeruginosa clinical isolates.

PubMed

Sun, Qinghui; Ba, Zhaofen; Wu, Guoying; Wang, Wei; Lin, Shuxiang; Yang, Hongjiang

2016-05-01

Carbapenem resistance mechanisms were investigated in 32 imipenem-resistant Pseudomonas aeruginosa clinical isolates recovered from hospitalised children. Sequence analysis revealed that 31 of the isolates had an insertion sequence element ISRP10 disrupting the porin gene oprD, demonstrating that ISRP10 inactivation of oprD conferred imipenem resistance in the majority of the isolates. Multilocus sequence typing (MLST) was used to discriminate the isolates. In total, 11 sequence types (STs) were identified including 3 novel STs, and 68.3% (28/41) of the tested strains were characterised as clone ST253. In combination with random amplified polymorphic DNA (RAPD) analysis, the imipenem-resistant isolates displayed a relatively high degree of genetic variability and were unlikely associated with nosocomial infections. Copyright © 2016 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

Metabolic Engineering of Yeast and Plants for the Production of the Biologically Active Hydroxystilbene, Resveratrol

PubMed Central

Jeandet, Philippe; Delaunois, Bertrand; Aziz, Aziz; Donnez, David; Vasserot, Yann; Cordelier, Sylvain; Courot, Eric

2012-01-01

Resveratrol, a stilbenic compound deriving from the phenyalanine/polymalonate route, being stilbene synthase the last and key enzyme of this pathway, recently has become the focus of a number of studies in medicine and plant physiology. Increased demand for this molecule for nutraceutical, cosmetic and possibly pharmaceutic uses, makes its production a necessity. In this context, the use of biotechnology through recombinant microorganisms and plants is particularly promising. Interesting results can indeed arise from the potential of genetically modified microorganisms as an alternative mechanism for producing resveratrol. Strategies used to tailoring yeast as they do not possess the genes that encode for the resveratrol pathway, will be described. On the other hand, most interest has centered in recent years, on STS gene transfer experiments from various origins to the genome of numerous plants. This work also presents a comprehensive review on plant molecular engineering with the STS gene, resulting in disease resistance against microorganisms and the enhancement of the antioxidant activities of several fruits in transgenic lines. PMID:22654481

Effect Of Spaceflight On Microbial Gene Expression And Virulence: Preliminary Results From Microbe Payload Flown On-Board STS-115

NASA Technical Reports Server (NTRS)

Wilson, J. W.; HonerzuBentrup, K,; Schurr, M. J.; Buchanan, K.; Morici, L.; Hammond, T.; Allen, P.; Baker, C.; Ott, C. M.; Nelman-Gonzalez M.;

Effective connectivity of facial expression network by using Granger causality analysis

NASA Astrophysics Data System (ADS)

Zhang, Hui; Li, Xiaoting

2013-10-01

Functional magnetic resonance imaging (fMRI) is an advanced non-invasive data acquisition technique to investigate the neural activity in human brain. In addition to localize the functional brain regions that is activated by specific cognitive task, fMRI can also be utilized to measure the task-related functional interactions among the active regions of interest (ROI) in the brain. Among the variety of analysis tools proposed for modeling the connectivity of brain regions, Granger causality analysis (GCA) measure the directions of information interactions by looking for the lagged effect among the brain regions. In this study, we use fMRI and Granger Causality analysis to investigate the effective connectivity of brain network induced by viewing several kinds of expressional faces. We focus on four kinds of facial expression stimuli: fearful, angry, happy and neutral faces. Five face selective regions of interest are localized and the effective connectivity within these regions is measured for the expressional faces. Our result based on 8 subjects showed that there is significant effective connectivity from STS to amygdala, from amygdala to OFA, aFFA and pFFA, from STS to aFFA and from pFFA to aFFA. This result suggested that there is an information flow from the STS to the amygdala when perusing expressional faces. This emotional expressional information flow that is conveyed by STS and amygdala, flow back to the face selective regions in occipital-temporal lobes, which constructed a emotional face processing network.

p53-dependent control of cell death by nicastrin: lack of requirement for presenilin-dependent gamma-secretase complex.

PubMed

Pardossi-Piquard, Raphaëlle; Dunys, Julie; Giaime, Emilie; Guillot-Sestier, Marie-Victoire; St George-Hyslop, Peter; Checler, Frédéric; Alves da Costa, Cristine

2009-04-01

Nicastrin (NCT) is a component of the presenilin (PS)-dependent gamma-secretase complexes that liberate amyloid beta-peptides from the beta-Amyloid Precursor Protein. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. Here we show that over-expression of NCT increases the viability of human embryonic kidney (HEK293) cells and decreases staurosporine (STS)- and thapsigargin (TPS)-induced caspase-3 activation in various cell lines from human and neuronal origins by Akt-dependent pathway. NCT lowers p53 expression, transcriptional activity and promoter transactivation and reduces p53 phosphorylation. NCT-associated protection against STS-stimulated cell death was completely abolished by p53 deficiency. Conversely, the depletion of NCT drastically enhances STS-induced caspase-3 activation and p53 pathway and favored p53 nuclear translocation. We examined whether NCT protective function depends on PS-dependent gamma-secretase activity. First, a 29-amino acid deletion known to reduce NCT-dependent amyloid beta-peptide production did not affect NCT-associated protective phenotype. Second, NCT still reduces STS-induced caspase-3 activation in fibroblasts lacking PS1 and PS2. Third, the gamma-secretase inhibitor DFK167 did not affect NCT-mediated reduction of p53 activity. Altogether, our study indicates that NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes.

Aberrant activity and connectivity of the posterior superior temporal sulcus during social cognition in schizophrenia.

PubMed

Mier, Daniela; Eisenacher, Sarah; Rausch, Franziska; Englisch, Susanne; Gerchen, Martin Fungisai; Zamoscik, Vera; Meyer-Lindenberg, Andreas; Zink, Mathias; Kirsch, Peter

2017-10-01

Schizophrenia is associated with significant impairments in social cognition. These impairments have been shown to go along with altered activation of the posterior superior temporal sulcus (pSTS). However, studies that investigate connectivity of pSTS during social cognition in schizophrenia are sparse. Twenty-two patients with schizophrenia and 22 matched healthy controls completed a social-cognitive task for functional magnetic resonance imaging that allows the investigation of affective Theory of Mind (ToM), emotion recognition and the processing of neutral facial expressions. Moreover, a resting-state measurement was taken. Patients with schizophrenia performed worse in the social-cognitive task (main effect of group). In addition, a group by social-cognitive processing interaction was revealed for activity, as well as for connectivity during the social-cognitive task, i.e., patients with schizophrenia showed hyperactivity of right pSTS during neutral face processing, but hypoactivity during emotion recognition and affective ToM. In addition, hypoconnectivity between right and left pSTS was revealed for affective ToM, but not for neutral face processing or emotion recognition. No group differences in connectivity from right to left pSTS occurred during resting state. This pattern of aberrant activity and connectivity of the right pSTS during social cognition might form the basis of false-positive perceptions of emotions and intentions and could contribute to the emergence and sustainment of delusions.

High-throughput analysis of sulfatides in cerebrospinal fluid using automated extraction and UPLC-MS/MS.

PubMed

Blomqvist, Maria; Borén, Jan; Zetterberg, Henrik; Blennow, Kaj; Månsson, Jan-Eric; Ståhlman, Marcus

2017-07-01

Sulfatides (STs) are a group of glycosphingolipids that are highly expressed in brain. Due to their importance for normal brain function and their potential involvement in neurological diseases, development of accurate and sensitive methods for their determination is needed. Here we describe a high-throughput oriented and quantitative method for the determination of STs in cerebrospinal fluid (CSF). The STs were extracted using a fully automated liquid/liquid extraction method and quantified using ultra-performance liquid chromatography coupled to tandem mass spectrometry. With the high sensitivity of the developed method, quantification of 20 ST species from only 100 μl of CSF was performed. Validation of the method showed that the STs were extracted with high recovery (90%) and could be determined with low inter- and intra-day variation. Our method was applied to a patient cohort of subjects with an Alzheimer's disease biomarker profile. Although the total ST levels were unaltered compared with an age-matched control group, we show that the ratio of hydroxylated/nonhydroxylated STs was increased in the patient cohort. In conclusion, we believe that the fast, sensitive, and accurate method described in this study is a powerful new tool for the determination of STs in clinical as well as preclinical settings. Copyright © 2017 by the American Society for Biochemistry and Molecular Biology, Inc.

Glutamate Concentration in the Superior Temporal Sulcus Relates to Neuroticism in Schizophrenia.

PubMed

Balz, Johanna; Roa Romero, Yadira; Keil, Julian; Schubert, Florian; Ittermann, Bernd; Mekle, Ralf; Montag, Christiane; Gallinat, Jürgen; Senkowski, Daniel

2018-01-01

Clinical studies suggest aberrant neurotransmitter concentrations in the brains of patients with schizophrenia (SCZ). Numerous studies have indicated deviant glutamate concentrations in SCZ, although the findings are inconsistent. Moreover, alterations in glutamate concentrations could be linked to personality traits in SCZ. Here, we examined the relationships between personality dimensions and glutamate concentrations in a voxel encompassing the occipital cortex (OCC) and another voxel encompassing the left superior temporal sulcus (STS). We used proton magnetic resonance spectroscopy to examine glutamate concentrations in the OCC and the STS in 19 SCZ and 21 non-psychiatric healthy control (HC) participants. Personality dimensions neuroticism, extraversion, openness, agreeableness and conscientiousness were assessed using the NEO-FFI questionnaire. SCZ compared to HC showed higher glutamate concentrations in the STS, reduced extraversion scores, and enhanced neuroticism scores. No group differences were observed for the other personality traits and for glutamate concentrations in the OCC. For the SCZ group, glutamate concentrations in STS were negatively correlated with the neuroticism scores [ r = -0.537, p = 0.018] but this was not found in HC [ r (19) = 0.011, p = 0.962]. No other significant correlations were found. Our study showed an inverse relationship between glutamate concentrations in the STS and neuroticism scores in SCZ. Elevated glutamate in the STS might serve as a compensatory mechanism that enables patients with enhanced concentrations to control and prevent the expression of neuroticism.

Glutamate Concentration in the Superior Temporal Sulcus Relates to Neuroticism in Schizophrenia

PubMed Central

Balz, Johanna; Roa Romero, Yadira; Keil, Julian; Schubert, Florian; Ittermann, Bernd; Mekle, Ralf; Montag, Christiane; Gallinat, Jürgen; Senkowski, Daniel

2018-01-01

Clinical studies suggest aberrant neurotransmitter concentrations in the brains of patients with schizophrenia (SCZ). Numerous studies have indicated deviant glutamate concentrations in SCZ, although the findings are inconsistent. Moreover, alterations in glutamate concentrations could be linked to personality traits in SCZ. Here, we examined the relationships between personality dimensions and glutamate concentrations in a voxel encompassing the occipital cortex (OCC) and another voxel encompassing the left superior temporal sulcus (STS). We used proton magnetic resonance spectroscopy to examine glutamate concentrations in the OCC and the STS in 19 SCZ and 21 non-psychiatric healthy control (HC) participants. Personality dimensions neuroticism, extraversion, openness, agreeableness and conscientiousness were assessed using the NEO-FFI questionnaire. SCZ compared to HC showed higher glutamate concentrations in the STS, reduced extraversion scores, and enhanced neuroticism scores. No group differences were observed for the other personality traits and for glutamate concentrations in the OCC. For the SCZ group, glutamate concentrations in STS were negatively correlated with the neuroticism scores [r = -0.537, p = 0.018] but this was not found in HC [r(19) = 0.011, p = 0.962]. No other significant correlations were found. Our study showed an inverse relationship between glutamate concentrations in the STS and neuroticism scores in SCZ. Elevated glutamate in the STS might serve as a compensatory mechanism that enables patients with enhanced concentrations to control and prevent the expression of neuroticism. PMID:29867621

KSC-2011-1796

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, media gather outside of Orbiter Processing Facility-2 to photograph space shuttle Endeavour's move, or "rollover," to the Vehicle Assembly Building (VAB). In the VAB, Endeavour will be lifted into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final and upcoming STS-134 mission. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

KSC-2011-1793

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, space shuttle Endeavour approaches the Vehicle Assembly Building, or VAB, on its move from Orbiter Processing Facility-2 where it was processed for its final and upcoming STS-134 mission. In the VAB, Endeavour will be lifted into a high bay where it will be joined to its external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1794

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, media gather outside of Orbiter Processing Facility-2 to photograph space shuttle Endeavour's move, or "rollover," to the Vehicle Assembly Building (VAB). In the VAB, Endeavour will be lifted into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final and upcoming STS-134 mission. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

KSC-2011-1900

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, space shuttle Endeavour is secured to a transporter for its move, or "rollover" from Orbiter Processing Facility-2 to the Vehicle Assembly Building (VAB). In the VAB, Endeavour will be lifted into a high bay where it will be attached to its external fuel tank and solid rocket boosters for its final mission, STS-134. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-3592

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Rising on twin columns of fire, space shuttle Endeavour lifts off from Launch Pad 39A at NASA's Kennedy Space Center in Florida beginning its final flight, the STS-134 mission, to the International Space Station. Launch was on time at 8:56 a.m. EDT on May 16. STS-134 and its six-member crew will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the space station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

KSC-2011-3589

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Kicking up a trail of smoke and steam, space shuttle Endeavour lifts off from Launch Pad 39A at NASA's Kennedy Space Center in Florida beginning its final flight, the STS-134 mission, to the International Space Station. Launch was on time at 8:56 a.m. EDT on May 16. STS-134 and its six-member crew will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the space station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

KSC-2011-3593

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Rising on twin columns of fire, space shuttle Endeavour lifts off from Launch Pad 39A at NASA's Kennedy Space Center in Florida beginning its final flight, the STS-134 mission, to the International Space Station. Launch was on time at 8:56 a.m. EDT on May 16. STS-134 and its six-member crew will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the space station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

Extensive Within-Host Diversity in Fecally Carried Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolates: Implications for Transmission Analyses.

PubMed

Stoesser, N; Sheppard, A E; Moore, C E; Golubchik, T; Parry, C M; Nget, P; Saroeun, M; Day, N P J; Giess, A; Johnson, J R; Peto, T E A; Crook, D W; Walker, A S

2015-07-01

Studies of the transmission epidemiology of antimicrobial-resistant Escherichia coli, such as strains harboring extended-spectrum beta-lactamase (ESBL) genes, frequently use selective culture of rectal surveillance swabs to identify isolates for molecular epidemiological investigation. Typically, only single colonies are evaluated, which risks underestimating species diversity and transmission events. We sequenced the genomes of 16 E. coli colonies from each of eight fecal samples (n = 127 genomes; one failure), taken from different individuals in Cambodia, a region of high ESBL-producing E. coli prevalence. Sequence data were used to characterize both the core chromosomal diversity of E. coli isolates and their resistance/virulence gene content as a proxy measure of accessory genome diversity. The 127 E. coli genomes represented 31 distinct sequence types (STs). Seven (88%) of eight subjects carried ESBL-positive isolates, all containing blaCTX-M variants. Diversity was substantial, with a median of four STs/individual (range, 1 to 10) and wide genetic divergence at the nucleotide level within some STs. In 2/8 (25%) individuals, the same blaCTX-M variant occurred in different clones, and/or different blaCTX-M variants occurred in the same clone. Patterns of other resistance genes and common virulence factors, representing differences in the accessory genome, were also diverse within and between clones. The substantial diversity among intestinally carried ESBL-positive E. coli bacteria suggests that fecal surveillance, particularly if based on single-colony subcultures, will likely underestimate transmission events, especially in high-prevalence settings. Copyright © 2015, Stoesser et al.

Systematic characterization of Bacillus Genetic Stock Center Bacillus thuringiensis strains using Multi-Locus Sequence Typing.

PubMed

Wang, Kui; Shu, Changlong; Soberón, Mario; Bravo, Alejandra; Zhang, Jie

2018-04-30

The goal of this work was to perform a systematic characterization of Bacillus thuringiensis (Bt) strains from the Bacillus Genetic Stock Center (BGSC) collection using Multi-Locus Sequence Typing (MLST). Different genetic markers of 158 Bacillus thuringiensis (Bt) strains from 73 different serovars stored in the BGSC, that represented 92% of the different Bt serovars of the BGSC were analyzed, the 8% that were not analyzed were not available. In addition, we analyzed 72 Bt strains from 18 serovars available at the pubMLST bcereus database, and Bt strains G03, HBF18 and Bt185, with no H serovars provided by our laboratory. We performed a systematic MLST analysis using seven housekeeping genes (glpF, gmK, ilvD, pta, pur, pycA and tpi) and analyzed correlation of the results of this analysis with strain serovars. The 233 Bt strains analyzed were assigned to 119 STs from which 19 STs were new. Genetic relationships were established by phylogenetic analysis and showed that STs could be grouped in two major Clusters containing 21 sub-groups. We found that a significant number of STs (101 in total) correlated with specific serovars, such as ST13 that corresponded to nine Bt isolates from B. thuringiensis serovar kenyae. However, other serovars showed high genetic variability and correlated with multiple STs; for example, B. thuringiensis serovar morrisoni correlated with 11 different STs. In addition, we found that 16 different STs correlated with multiple serovars (2-4 different serovars); for example, ST12 correlated with B. thuringiensis serovar alesti, dakota, palmanyolensis and sotto/dendrolimus. These data indicated that only partial correspondence between MLST and serotyping can be established. Copyright © 2018 Elsevier Inc. All rights reserved.

Biosynthesis of the Major Tetrahydroxystilbenes in Spruce, Astringin and Isorhapontin, Proceeds via Resveratrol and Is Enhanced by Fungal Infection1[W][OA

PubMed Central

Hammerbacher, Almuth; Ralph, Steven G.; Bohlmann, Joerg; Fenning, Trevor M.; Gershenzon, Jonathan; Schmidt, Axel

2011-01-01

Stilbenes are dibenzyl polyphenolic compounds produced in several unrelated plant families that appear to protect against various biotic and abiotic stresses. Stilbene biosynthesis has been well described in economically important plants, such as grape (Vitis vinifera), peanut (Arachis hypogaea), and pine (Pinus species). However, very little is known about the biosynthesis and ecological role of stilbenes in spruce (Picea), an important gymnosperm tree genus in temperate and boreal forests. To investigate the biosynthesis of stilbenes in spruce, we identified two similar stilbene synthase (STS) genes in Norway spruce (Picea abies), PaSTS1 and PaSTS2, which had orthologs with high sequence identity in sitka (Picea sitchensis) and white (Picea glauca) spruce. Despite the conservation of STS sequences in these three spruce species, they differed substantially from angiosperm STSs. Several types of in vitro and in vivo assays revealed that the P. abies STSs catalyze the condensation of p-coumaroyl-coenzyme A and three molecules of malonyl-coenzyme A to yield the trihydroxystilbene resveratrol but do not directly form the dominant spruce stilbenes, which are tetrahydroxylated. However, in transgenic Norway spruce overexpressing PaSTS1, significantly higher amounts of the tetrahydroxystilbene glycosides, astringin and isorhapontin, were produced. This result suggests that the first step of stilbene biosynthesis in spruce is the formation of resveratrol, which is further modified by hydroxylation, O-methylation, and O-glucosylation to yield astringin and isorhapontin. Inoculating spruce with fungal mycelium increased STS transcript abundance and tetrahydroxystilbene glycoside production. Extracts from STS-overexpressing lines significantly inhibited fungal growth in vitro compared with extracts from control lines, suggesting that spruce stilbenes have a role in antifungal defense. PMID:21865488

Biosynthesis of the major tetrahydroxystilbenes in spruce, astringin and isorhapontin, proceeds via resveratrol and is enhanced by fungal infection.

PubMed

Hammerbacher, Almuth; Ralph, Steven G; Bohlmann, Joerg; Fenning, Trevor M; Gershenzon, Jonathan; Schmidt, Axel

2011-10-01

Stilbenes are dibenzyl polyphenolic compounds produced in several unrelated plant families that appear to protect against various biotic and abiotic stresses. Stilbene biosynthesis has been well described in economically important plants, such as grape (Vitis vinifera), peanut (Arachis hypogaea), and pine (Pinus species). However, very little is known about the biosynthesis and ecological role of stilbenes in spruce (Picea), an important gymnosperm tree genus in temperate and boreal forests. To investigate the biosynthesis of stilbenes in spruce, we identified two similar stilbene synthase (STS) genes in Norway spruce (Picea abies), PaSTS1 and PaSTS2, which had orthologs with high sequence identity in sitka (Picea sitchensis) and white (Picea glauca) spruce. Despite the conservation of STS sequences in these three spruce species, they differed substantially from angiosperm STSs. Several types of in vitro and in vivo assays revealed that the P. abies STSs catalyze the condensation of p-coumaroyl-coenzyme A and three molecules of malonyl-coenzyme A to yield the trihydroxystilbene resveratrol but do not directly form the dominant spruce stilbenes, which are tetrahydroxylated. However, in transgenic Norway spruce overexpressing PaSTS1, significantly higher amounts of the tetrahydroxystilbene glycosides, astringin and isorhapontin, were produced. This result suggests that the first step of stilbene biosynthesis in spruce is the formation of resveratrol, which is further modified by hydroxylation, O-methylation, and O-glucosylation to yield astringin and isorhapontin. Inoculating spruce with fungal mycelium increased STS transcript abundance and tetrahydroxystilbene glycoside production. Extracts from STS-overexpressing lines significantly inhibited fungal growth in vitro compared with extracts from control lines, suggesting that spruce stilbenes have a role in antifungal defense.

Mental retardation in a boy with an interstitial deletion at Xp22.3 involving STS, KAL1, and OA1: Implication for the MRX locus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Muroya, Koji; Ogata, Tsutomu; Natsuo, Nobutake

Although genotype-phenotype correlations in male patients with various types of nullisomy for Xp22.3 have assigned a locus for X-linked mental retardation (MRX) to an approximately 3-Mb region between DXS31 and STS, the precise location has not been determined. In this paper, we describe a 14 7/12 year old Japanese boy with mental retardation and an interstitial deletion at Xp22.3 involving STS, KAL1, and OA1, and compare the deletion map with that of previously reported three familial male patients with low-normal intelligence and a similar interstitial deletion at Xp22.3. The results suggest that the MRX gene is further localized to themore » roughly 1.5-Mb region between DXS1060 and DXS1139. 31 refs., 4 figs.« less

Lateralization for dynamic facial expressions in human superior temporal sulcus.

PubMed

De Winter, François-Laurent; Zhu, Qi; Van den Stock, Jan; Nelissen, Koen; Peeters, Ronald; de Gelder, Beatrice; Vanduffel, Wim; Vandenbulcke, Mathieu

2015-02-01

Most face processing studies in humans show stronger activation in the right compared to the left hemisphere. Evidence is largely based on studies with static stimuli focusing on the fusiform face area (FFA). Hence, the pattern of lateralization for dynamic faces is less clear. Furthermore, it is unclear whether this property is common to human and non-human primates due to predisposing processing strategies in the right hemisphere or that alternatively left sided specialization for language in humans could be the driving force behind this phenomenon. We aimed to address both issues by studying lateralization for dynamic facial expressions in monkeys and humans. Therefore, we conducted an event-related fMRI experiment in three macaques and twenty right handed humans. We presented human and monkey dynamic facial expressions (chewing and fear) as well as scrambled versions to both species. We studied lateralization in independently defined face-responsive and face-selective regions by calculating a weighted lateralization index (LIwm) using a bootstrapping method. In order to examine if lateralization in humans is related to language, we performed a separate fMRI experiment in ten human volunteers including a 'speech' expression (one syllable non-word) and its scrambled version. Both within face-responsive and selective regions, we found consistent lateralization for dynamic faces (chewing and fear) versus scrambled versions in the right human posterior superior temporal sulcus (pSTS), but not in FFA nor in ventral temporal cortex. Conversely, in monkeys no consistent pattern of lateralization for dynamic facial expressions was observed. Finally, LIwms based on the contrast between different types of dynamic facial expressions (relative to scrambled versions) revealed left-sided lateralization in human pSTS for speech-related expressions compared to chewing and emotional expressions. To conclude, we found consistent laterality effects in human posterior STS but not in visual cortex of monkeys. Based on our results, it is tempting to speculate that lateralization for dynamic face processing in humans may be driven by left-hemispheric language specialization which may not have been present yet in the common ancestor of human and macaque monkeys. Copyright © 2014 Elsevier Inc. All rights reserved.

Population structure of clinical Pseudomonas aeruginosa from West and Central African countries.

PubMed

Cholley, Pascal; Ka, Roughyatou; Guyeux, Christophe; Thouverez, Michelle; Guessennd, Nathalie; Ghebremedhin, Beniam; Frank, Thierry; Bertrand, Xavier; Hocquet, Didier

2014-01-01

Pseudomonas aeruginosa (PA) has a non-clonal, epidemic population with a few widely distributed and frequently encountered sequence types (STs) called 'high-risk clusters'. Clinical P. aeruginosa (clinPA) has been studied in all inhabited continents excepted in Africa, where a very few isolates have been analyzed. Here, we characterized a collection of clinPA isolates from four countries of West and Central Africa. 184 non-redundant isolates of clinPA from hospitals of Senegal, Ivory Coast, Nigeria, and Central African Republic were genotyped by MLST. We assessed their resistance level to antibiotics by agar diffusion and identified the extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs) by sequencing. The population structure of the species was determined by a nucleotide-based analysis of the entire PA MLST database and further localized on the phylogenetic tree (i) the sequence types (STs) of the present collection, (ii) the STs by continents, (iii) ESBL- and MBL-producing STs from the MLST database. We found 80 distinct STs, of which 24 had no relationship with any known STs. 'High-risk' international clonal complexes (CC155, CC244, CC235) were frequently found in West and Central Africa. The five VIM-2-producing isolates belonged to CC233 and CC244. GES-1 and GES-9 enzymes were produced by one CC235 and one ST1469 isolate, respectively. We showed the spread of 'high-risk' international clonal complexes, often described as multidrug-resistant on other continents, with a fully susceptible phenotype. The MBL- and ESBL-producing STs were scattered throughout the phylogenetic tree and our data suggest a poor association between a continent and a specific phylogroup. ESBL- and MBL-encoding genes are borne by both successful international clonal complexes and distinct local STs in clinPA of West and Central Africa. Furthermore, our data suggest that the spread of a ST could be either due to its antibiotic resistance or to features independent from the resistance to antibiotics.

KSC-2009-4198

NASA Image and Video Library

2009-07-16

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians keep watch as the control moment gyroscope is lifted from its stand. It will be moved to an EXPRESS Logistics Carrier. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12 . Photo credit: NASA/Jack Pfaller

KSC-2009-4635

NASA Image and Video Library

2009-08-12

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, a control moment gyroscope is lifted by crane above an EXPRESS Logistics Carrier on which it will be installed for flight. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4199

NASA Image and Video Library

2009-07-16

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians keep watch as the control moment gyroscope is lifted past the Node 3 Tranquility module to an EXPRESS Logistics Carrier. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12 . Photo credit: NASA/Jack Pfaller

KSC-2011-1791

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, workers accompany space shuttle Endeavour as it is being transported from Orbiter Processing Facility-2 to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1792

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, workers accompany space shuttle Endeavour as it is being transported from Orbiter Processing Facility-2 to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2009-4708

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians prepare to lift the nitrogen tank assembly to move it to the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4710

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians watch closely as an overhead crane lifts the nitrogen tank assembly to move it to the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4709

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians check the nitrogen tank assembly before lifting and moving it to the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4711

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians check the nitrogen tank assembly closely as an overhead crane lifts and moves it to the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4712

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians check the nitrogen tank assembly closely as an overhead crane lifts and moves it to the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4713

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians check the nitrogen tank assembly closely as an overhead crane lifts and moves it to the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4718

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians check the placement of the nitrogen tank assembly on the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-4717

NASA Image and Video Library

2009-08-17

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, technicians check closely as the nitrogen tank assembly is lowered closer to the Express Logistics Carrier 1, or ELC-1. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jim Grossmann

KSC-2009-2243

NASA Image and Video Library

2009-03-21

CAPE CANAVERAL, Fla. – Inside the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, workers begin removing the shipping container from around the EXPRESS Logistics Carrier for the STS-129 mission. The carrier is part of the payload on space shuttle Atlantis, which will deliver to the International Space Station components including two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Tim Jacobs

KSC-2009-2239

NASA Image and Video Library

2009-03-21

CAPE CANAVERAL, Fla. – On the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, a truck carrying the EXPRESS Logistics Carrier for the STS-129 mission drives out of the open rear of the C-17 cargo plane. The carrier is part of the payload on space shuttle Atlantis, which will deliver to the International Space Station components including two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Tim Jacobs

KSC-2009-2240

NASA Image and Video Library

2009-03-21

CAPE CANAVERAL, Fla. – The truck carrying the EXPRESS Logistics Carrier for the STS-129 mission pulls away from the C-17 cargo plane that delivered it to NASA's Kennedy Space Center in Florida. The carrier is part of the payload on space shuttle Atlantis, which will deliver to the International Space Station components including two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Tim Jacobs

Development and Characterization of a Psathyrostachys huashanica Keng 7Ns Chromosome Addition Line with Leaf Rust Resistance

PubMed Central

Du, Wanli; Wang, Jing; Wang, Liangming; Zhang, Jun; Chen, Xinhong; Zhao, Jixin; Yang, Qunhui; Wu, Jun

2013-01-01

The aim of this study was to characterize a Triticum aestivum-Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) disomic addition line 2-1-6-3. Individual line 2-1-6-3 plants were analyzed using cytological, genomic in situ hybridization (GISH), EST-SSR, and EST-STS techniques. The alien addition line 2-1-6-3 was shown to have two P. huashanica chromosomes, with a meiotic configuration of 2n = 44 = 22 II. We tested 55 EST-SSR and 336 EST-STS primer pairs that mapped onto seven different wheat chromosomes using DNA from parents and the P. huashanica addition line. One EST-SSR and nine EST-STS primer pairs indicated that the additional chromosome of P. huashanica belonged to homoeologous group 7, the diagnostic fragments of five EST-STS markers (BE404955, BE591127, BE637663, BF482781 and CD452422) were cloned, sequenced and compared. The results showed that the amplified polymorphic bands of P. huashanica and disomic addition line 2-1-6-3 shared 100% sequence identity, which was designated as the 7Ns disomic addition line. Disomic addition line 2-1-6-3 was evaluated to test the leaf rust resistance of adult stages in the field. We found that one pair of the 7Ns genome chromosomes carried new leaf rust resistance gene(s). Moreover, wheat line 2-1-6-3 had a superior numbers of florets and grains per spike, which were associated with the introgression of the paired P. huashanica chromosomes. These high levels of disease resistance and stable, excellent agronomic traits suggest that this line could be utilized as a novel donor in wheat breeding programs. PMID:23976963

Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil.

PubMed

Valença Barbosa, Carolina; de Jesus Batista, Rosemary; Pereira Igreja, Ricardo; d'Avila Levy, Claudia Masini; Werneck de Macedo, Heloisa; Carneiro Santos, Helena Lúcia

2017-10-25

Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil. A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers. Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%). DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro.

In Utero Bisphenol A Concentration, Metabolism, and Global DNA Methylation Across Matched Placenta, Kidney, and Liver in the Human Fetus

PubMed Central

Nahar, Muna S.; Liao, Chunyang; Kannan, Kurunthachalam; Harris, Craig; Dolinoy, Dana C.

2014-01-01

While urine has been an easily accessible and feasible matrix for human biomonitoring, analytical measurements in internal tissues and organs can provide more accurate exposure assessments to understand disease etiology. This is especially important for the endocrine active compound, bisphenol A (BPA), where studies investigating internal doses at sensitive periods of human development are currently lacking. Herein, BPA concentrations, BPA-specific metabolizing enzyme gene expression, and global DNA methylation were characterized across three matched tissues from elective pregnancy terminations of 2nd trimester human fetuses: the placenta, liver, and kidney (N=12 each; N=36 total). Compared to liver (free: 0.54-50.5 ng/g), BPA concentrations were lower in matched placenta (<0.05-25.4 ng/g) and kidney (0.08-11.1 ng/g) specimens. BPA-specific metabolism gene expression of GUSB, UGT2B15, STS, and SULT1A1 differed across each tissue type; however, conjugation and deconjugation expression patterns were similar across the fetus. Average LINE1 and CCGG global methylation were 58.3 and 59.2% in placenta, 79.5 and 66.4% in fetal liver, and 77.9 and 77.0% in fetal kidney, with significant tissue-specific DNA methylation differences in both LINE1 (p-value <0.001) and CCGG content (p-value <0.001). Total BPA concentrations were positively associated with global methylation for the placenta only using the LINE1 assay (p-value: 0.002), suggesting organ-specific biological effects after fetal exposure. Utilizing sensitive human clinical specimens, results are informative for BPA toxicokinetics and toxicodynamics assessment in the developing human fetus. PMID:25434263

A genetic variant within STS previously associated with inattention in boys with attention deficit hyperactivity disorder is associated with enhanced cognition in healthy adult males.

PubMed

Humby, Trevor; Fisher, Amelia; Allen, Christopher; Reynolds, Meghann; Hartman, Annette; Giegling, Ina; Rujescu, Dan; Davies, William

2017-03-01

The enzyme steroid sulfatase (STS) converts sulfated steroids to their non-sulfated forms. Deficiency for this enzyme is associated with inattention but preserved response control. The polymorphism rs17268988 within the X-linked STS gene is associated with inattentive, but not other, symptoms in boys with attention deficit hyperactivity disorder (ADHD). We initially tested whether rs17268988 genotype was associated with attention, response control, and underlying aspects of cognition, using questionnaires and neuropsychological tasks, in two independent cohorts of healthy adult males. In an additional analysis based upon existing data, the performance of mice with genetic or pharmacological manipulations of the STS axis under attentionally demanding conditions was investigated. G-allele carriers at rs17268988 exhibited reduced reaction time, enhanced attention, and reduced reaction time variability relative to C-allele carriers. Mice with genetic or pharmacological manipulations of the STS axis were shown to have perturbed reaction time variability. Our findings provide additional support for an association between rs17268988 genotype and attention, which may be partially mediated by reaction time variability; they also indicate that, in contrast to the situation in boys with ADHD, in healthy men, the G-allele at rs17268988 is associated with enhanced cognition. As reaction time variability is a predictor of well-being, rs17268988 genotype may represent a biomarker for long-term health.

KSC-2011-3591

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Rising on twin columns of fire and kicking up a trail of smoke and steam, space shuttle Endeavour lifts off from Launch Pad 39A at NASA's Kennedy Space Center in Florida beginning its final flight, the STS-134 mission, to the International Space Station. Launch was on time at 8:56 a.m. EDT on May 16. STS-134 and its six-member crew will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the space station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

KSC-2011-3590

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Rising on twin columns of fire and kicking up a trail of smoke and steam, space shuttle Endeavour lifts off from Launch Pad 39A at NASA's Kennedy Space Center in Florida beginning its final flight, the STS-134 mission, to the International Space Station. Launch was on time at 8:56 a.m. EDT on May 16. STS-134 and its six-member crew will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the space station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-074 (1 June 2011) --- Space shuttle Endeavour rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Heat from the shuttle's auxiliary power units, which provide hydraulic control, can be seen at the back of Endeavour, near the vertical tail. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-088 (1 June 2011) --- Space shuttle Endeavour rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Heat from the shuttle's auxiliary power units, which provide hydraulic control, can be seen at the back of Endeavour, near the vertical tail. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-087 (1 June 2011) --- Space shuttle Endeavour rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Heat from the shuttle's auxiliary power units, which provide hydraulic control, can be seen at the back of Endeavour, near the vertical tail. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-085 (1 June 2011) --- Space shuttle Endeavour rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Heat from the shuttle's auxiliary power units, which provide hydraulic control, can be seen at the back of Endeavour, near the vertical tail. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

Final Landing of the Space Shuttle Endeavour / STS-134 Mission

NASA Image and Video Library

2011-06-01

STS134-S-086 (1 June 2011) --- Space shuttle Endeavour rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Heat from the shuttle's auxiliary power units, which provide hydraulic control, can be seen at the back of Endeavour, near the vertical tail. Main gear touchdown was at 2:34:51 a.m. (EDT) on June 1, 2011, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. Onboard are NASA astronauts Mark Kelly, STS-134 commander; Greg H. Johnson, pilot; Michael Fincke, Andrew Feustel, Greg Chamitoff and European Space Agency astronaut Roberto Vittori, all mission specialists. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

KSC-2009-6402

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - A post-launch news conference is held in the NASA Press Site auditorium at NASA's Kennedy Space Center in Florida after the successful launch of space shuttle Atlantis. From left are Public Affairs moderator Mike Curie; Bill Gerstenmaier, associate administrator for Space Operations; Mike Moses, chair, Mission Management Team; and Mike Leinbach, space shuttle launch director. Liftoff of Atlantis on its STS-129 mission came at 2:28 p.m. EST Nov. 16 from Launch Pad 39A. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany.

PubMed

Guenther, Sebastian; Bethe, Astrid; Fruth, Angelika; Semmler, Torsten; Ulrich, Rainer G; Wieler, Lothar H; Ewers, Christa

2012-01-01

Urban rats present a global public health concern as they are considered a reservoir and vector of zoonotic pathogens, including Escherichia coli. In view of the increasing emergence of antimicrobial resistant E. coli strains and the on-going discussion about environmental reservoirs, we intended to analyse whether urban rats might be a potential source of putatively zoonotic E. coli combining resistance and virulence. For that, we took fecal samples from 87 brown rats (Rattus norvegicus) and tested at least three E. coli colonies from each animal. Thirty two of these E. coli strains were pre-selected from a total of 211 non-duplicate isolates based on their phenotypic resistance to at least three antimicrobial classes, thus fulfilling the definition of multiresistance. As determined by multilocus sequence typing (MLST), these 32 strains belonged to 24 different sequence types (STs), indicating a high phylogenetic diversity. We identified STs, which frequently occur among extraintestinal pathogenic E. coli (ExPEC), such as STs 95, 131, 70, 428, and 127. Also, the detection of a number of typical virulence genes confirmed that the rats tested carried ExPEC-like strains. In particular, the finding of an Extended-spectrum beta-lactamase (ESBL)-producing strain which belongs to a highly virulent, so far mainly human- and avian-restricted ExPEC lineage (ST95), which expresses a serogroup linked with invasive strains (O18:NM:K1), and finally, which produces an ESBL-type frequently identified among human strains (CTX-M-9), pointed towards the important role, urban rats might play in the transmission of multiresistant and virulent E. coli strains. Indeed, using a chicken infection model, this strain showed a high in vivo pathogenicity. Imagining the high numbers of urban rats living worldwide, the way to the transmission of putatively zoonotic, multiresistant, and virulent strains might not be far ahead. The unforeseeable consequences of such an emerging public health threat need careful consideration in the future.

Spaceflight Effects and Molecular Responses in the Mouse Eye: Observations After Shuttle Mission STS-133

NASA Technical Reports Server (NTRS)

Prospero-Ponce, Claudia; Zanello, Susana B.; CoreyTheriot, Patricia; Chevez-Barrios, P.

2012-01-01

Microgravity-induced cephalad fluid shift and radiation exposure are some of the stressors seen in space exploration. Ocular changes leading to visual impairment in astronauts are of occupational health relevance. Therefore, we analyzed the effects of space flight in the eyes of mice. Six mice were assigned to Flight (FLT), Animal enclosure Module (AEM), or vivarium (VIV) group, respectively. Mice were sacrificed at 1, 5 or 7 days after landing from space. One eye was used for histological and immunohistoche-mistry analysis and the other eye for gene expression profiling. 8-OHdG and caspase-3 immunoreactivity were increased in the retina in FLT samples at return(R+1) compared to AEM/VIV groups, and decreased at day 7 (R+7). beta-amyloid was seen in the nerve fibers at the post-laminar region of the optic nerve in the flight samples (R+7). In addition, oxidative and cellular stress response genes were upregulated in the retina of FLT samples upon landing, and decreased by R+7. According to the results, a reversible molecular damage may occur in the retina of mice exposed to spaceflight followed by protective cellular response.

Individual variation in the motivational and neurobiological effects of an opioid cue.

PubMed

Yager, Lindsay M; Pitchers, Kyle K; Flagel, Shelly B; Robinson, Terry E

2015-03-13

A discrete cue associated with intravenous injections of cocaine acquires greater control over motivated behavior in some rats ('sign-trackers', STs) than others ('goal-trackers', GTs). It is not known, however, if such variation generalizes to cues associated with other drugs. We asked, therefore, whether a discrete cue (a light) associated with the intravenous administration of an opioid drug (the short-acting mu receptor agonist, remifentanil) acquires incentive motivational properties differently in STs and GTs, as indicated by tests of Pavlovian conditioned approach and conditioned reinforcement. Consistent with studies using cocaine, STs approached a classically conditioned opioid cue more readily than GTs, and in a test of conditioned reinforcement worked more avidly to get it. Interestingly, STs and GTs did not differ in the acquisition of a conditioned orienting response. In addition, the performance of conditioned approach behavior, but not conditioned orientation, was attenuated by pretreatment with the dopamine receptor antagonist, flupenthixol, into the core of the nucleus accumbens. Lastly, food and opioid cues engaged similar amygdalo-striatal-thalamic circuitry to a much greater extent in STs than GTs, as indicated by Fos expression. Taken together, these data demonstrate that, similar to food and cocaine cues: (1) a discrete opioid cue attains greater incentive motivational value in STs than GTs; (2) the attribution of incentive motivational properties to an opioid cue is dopamine dependent; and (3) an opioid cue engages the so-called 'motive circuit' only if it is imbued with incentive salience.

Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival.

PubMed

Giebler, Maria; Staege, Martin S; Blauschmidt, Sindy; Ohm, Lea I; Kraus, Matthias; Würl, Peter; Taubert, Helge; Greither, Thomas

2018-01-01

A wide variety of endogenous retroviral sequences has been demonstrated in the human genome so far, divided into several different families according to the sequence homology to viral strains. While increased expression of human endogenous retrovirus (HERV) elements has already been linked to unfavorable prognosis in hepatocellular carcinoma, breast cancer, and ovarian carcinoma yet less is known about the impact of the expression of different HERV elements on sarcomagenesis in general as well as the outcome of soft tissue sarcoma (STS) patients. Therefore, in this study the association between expression of HERV-K and HERV-F and the clinicopathological characteristics in a cohort of STSs as well as the patients' prognosis was evaluated. HERV-K and HERV-F expression was assessed by quantitative real-time PCR in 120 patient specimens. HERV-K and HERV-F expression was significantly correlated ( r S = 0.5; p = 6.4 × 10 -9 ; Spearman's rank bivariate correlation). Also, tumor diameter exhibited a significant negative association to HERV-K and HERV-F expression. Levels of several hypoxia-related RNAs like HIF-1α and miR-210 showed a significant positive correlation with both HERV-K and HERV-F expression. Although in survival analyses no impact of HERV expression on disease-specific survival could be detected, patients with elevated HERV-K expression had a significantly shorter relapse-free survival ( p = 0.014, log-rank analysis). In conclusion, we provide evidence for the first time that the increased expression of HERV-K in tumors is associated with STS patients' prognosis.

KSC-2011-4205

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- The crew members of space shuttle Endeavour's STS-134 mission undergo brief medical checks in the Crew Transport Vehicle before talking to media gathered on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4209

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- STS-134 Mission Specialists Drew Feustel, left, and Roberto Vittori with the European Space Agency, right, admire space shuttle Endeavour with NASA astronaut Randy Bresnik at NASA's Kennedy Space Center in Florida. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4211

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- STS-134 Pilot Greg H. Johnson, left, and Commander Mark Kelly check out the nose of space shuttle Endeavour following the successful return to NASA's Kennedy Space Center in Florida. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4212

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- STS-134 Mission Specialist Mike Fincke, left, Pilot Greg H. Johnson and Commander Mark Kelly talk with employees following the successful return of space shuttle Endeavour to NASA's Kennedy Space Center in Florida. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4213

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- STS-134 Commander Mark Kelly, left, and NASA Administrator Charlie Bolden chat with employees following the successful return of space shuttle Endeavour to NASA's Kennedy Space Center in Florida. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-3198

NASA Image and Video Library

2011-04-29

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour's six STS-134 astronauts, dressed in their orange launch-and-entry suits, wave to news media and other spectators before hopping aboard the Astrovan parked in front of the Operations and Checkout Building at NASA's Kennedy Space Center in Florida. The modified Airstream recreational vehicle has transported astronauts to their spacecraft since 1984. From left, are Mission Specialists Greg Chamitoff, Andrew Feustel, Roberto Vittori with the European Space Agency and Michael Fincke, Pilot Greg H. Johnson and Commander Mark Kelly. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the space station. Endeavour was scheduled to launch at 3:47 p.m. on April 29, but that attempt was scrubbed for at least 72 hours while engineers assess an issue associated with the shuttle's Auxiliary Power Unit 1. STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3196

NASA Image and Video Library

2011-04-29

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour's six STS-134 astronauts, dressed in their orange launch-and-entry suits, wave to news media and other spectators as they walk toward the Astrovan parked in front of the Operations and Checkout Building at NASA's Kennedy Space Center in Florida. The modified Airstream recreational vehicle has transported astronauts to their spacecraft since 1984. Leading the way are Commander Mark Kelly, right, and Pilot Greg H. Johnson. Behind Kelly are crewmates Michael Fincke and Andrew Feustel. Behind Johnson are crewmates Roberto Vittori with the European Space Agency and Greg Chamitoff. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the space station. Endeavour was scheduled to launch at 3:47 p.m. on April 29, but that attempt was scrubbed for at least 72 hours while engineers assess an issue associated with the shuttle's Auxiliary Power Unit 1. STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3197

NASA Image and Video Library

2011-04-29

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour's six STS-134 astronauts, dressed in their orange launch-and-entry suits, wave to news media and other spectators as they walk toward the Astrovan parked in front of the Operations and Checkout Building at NASA's Kennedy Space Center in Florida. The modified Airstream recreational vehicle has transported astronauts to their spacecraft since 1984. Leading the way is Commander Mark Kelly, followed by crewmates Greg H. Johnson, Michael Fincke, Andrew Feustel, Roberto Vittori with the European Space Agency and Greg Chamitoff. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the space station. Endeavour was scheduled to launch at 3:47 p.m. on April 29, but that attempt was scrubbed for at least 72 hours while engineers assess an issue associated with the shuttle's Auxiliary Power Unit 1. STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

Network Interactions Explain Sensitivity to Dynamic Faces in the Superior Temporal Sulcus.

PubMed

Furl, Nicholas; Henson, Richard N; Friston, Karl J; Calder, Andrew J

2015-09-01

The superior temporal sulcus (STS) in the human and monkey is sensitive to the motion of complex forms such as facial and bodily actions. We used functional magnetic resonance imaging (fMRI) to explore network-level explanations for how the form and motion information in dynamic facial expressions might be combined in the human STS. Ventral occipitotemporal areas selective for facial form were localized in occipital and fusiform face areas (OFA and FFA), and motion sensitivity was localized in the more dorsal temporal area V5. We then tested various connectivity models that modeled communication between the ventral form and dorsal motion pathways. We show that facial form information modulated transmission of motion information from V5 to the STS, and that this face-selective modulation likely originated in OFA. This finding shows that form-selective motion sensitivity in the STS can be explained in terms of modulation of gain control on information flow in the motion pathway, and provides a substantial constraint for theories of the perception of faces and biological motion. © The Author 2014. Published by Oxford University Press.

Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli.

PubMed

Watts, Kevin T; Lee, Pyung C; Schmidt-Dannert, Claudia

2006-03-21

Phenylpropanoids are the precursors to a range of important plant metabolites such as the cell wall constituent lignin and the secondary metabolites belonging to the flavonoid/stilbene class of compounds. The latter class of plant natural products has been shown to function in a wide range of biological activities. During the last few years an increasing number of health benefits have been associated with these compounds. In particular, they demonstrate potent antioxidant activity and the ability to selectively inhibit certain tyrosine kinases. Biosynthesis of many medicinally important plant secondary metabolites, including stilbenes, is frequently not very well understood and under tight spatial and temporal control, limiting their availability from plant sources. As an alternative, we sought to develop an approach for the biosynthesis of diverse stilbenes by engineered recombinant microbial cells. A pathway for stilbene biosynthesis was constructed in Escherichia coli with 4-coumaroyl CoA ligase 1 4CL1) from Arabidopsis thaliana and stilbene synthase (STS) cloned from Arachis hypogaea. E. coli cultures expressing these enzymes together converted the phenylpropionic acid precursor 4-coumaric acid, added to the growth medium, to the stilbene resveratrol (>100 mg/L). Caffeic acid, added in the same way, resulted in the production of the expected dihydroxylated stilbene, piceatannol (>10 mg/L). Ferulic acid, however, was not converted to the expected stilbene product, isorhapontigenin. Substitution of 4CL1 with a homologous enzyme, 4CL4, with a preference for ferulic acid over 4-coumaric acid, had no effect on the conversion of ferulic acid. Accumulation of tri- and tetraketide lactones from ferulic acid, regardless of the CoA-ligase expressed in E. coli, suggests that STS cannot properly accommodate and fold the tetraketide intermediate to the corresponding stilbene structure. Phenylpropionic acids, such as 4-coumaric acid and caffeic acid, can be efficiently converted to stilbene compounds by recombinant E. coli cells expressing plant biosynthetic genes. Optimization of precursor conversion and cyclization of the bulky ferulic acid precursor by host metabolic engineering and protein engineering may afford the synthesis of even more structurally diverse stilbene compounds.

Identification of Putative Precursor Genes for the Biosynthesis of Cannabinoid-Like Compound in Radula marginata

PubMed Central

Hussain, Tajammul; Plunkett, Blue; Ejaz, Mahwish; Espley, Richard V.; Kayser, Oliver

2018-01-01

The liverwort Radula marginata belongs to the bryophyte division of land plants and is a prospective alternate source of cannabinoid-like compounds. However, mechanistic insights into the molecular pathways directing the synthesis of these cannabinoid-like compounds have been hindered due to the lack of genetic information. This prompted us to do deep sequencing, de novo assembly and annotation of R. marginata transcriptome, which resulted in the identification and validation of the genes for cannabinoid biosynthetic pathway. In total, we have identified 11,421 putative genes encoding 1,554 enzymes from 145 biosynthetic pathways. Interestingly, we have identified all the upstream genes of the central precursor of cannabinoid biosynthesis, cannabigerolic acid (CBGA), including its two first intermediates, stilbene acid (SA) and geranyl diphosphate (GPP). Expression of all these genes was validated using quantitative real-time PCR. We have characterized the protein structure of stilbene synthase (STS), which is considered as a homolog of olivetolic acid in R. marginata. Moreover, the metabolomics approach enabled us to identify CBGA-analogous compounds using electrospray ionization mass spectrometry (ESI-MS/MS) and gas chromatography mass spectrometry (GC-MS). Transcriptomic analysis revealed 1085 transcription factors (TF) from 39 families. Comparative analysis showed that six TF families have been uniquely predicted in R. marginata. In addition, the bioinformatics analysis predicted a large number of simple sequence repeats (SSRs) and non-coding RNAs (ncRNAs). Our results collectively provide mechanistic insights into the putative precursor genes for the biosynthesis of cannabinoid-like compounds and a novel transcriptomic resource for R. marginata. The large-scale transcriptomic resource generated in this study would further serve as a reference transcriptome to explore the Radulaceae family.

Sodium tanshinone IIA silate inhibits oxygen-glucose deprivation/recovery-induced cardiomyocyte apoptosis via suppression of the NF-κB/TNF-α pathway

PubMed Central

Wu, Wen-Yu; Wang, Wen-Yi; Ma, Yan-Ling; Yan, Hong; Wang, Xin-Bo; Qin, Yin-Lin; Su, Mei; Chen, Tao; Wang, Yi-Ping

2013-01-01

Background and Purpose Inhibition of apoptosis may attenuate the irreversible injury associated with reperfusion. In the current study, we focused on the cytoprotective effects and the underlying mechanism of sodium tanshinone IIA silate (STS) against damage induced by oxygen-glucose deprivation/recovery (OGD/R). in H9c2 cardiomyocytes and the underlying mechanisms. Experimental Approach We used a model of cardiac ischaemia/reperfusion, OGD/R in H9c2 cardiomyocytes, to assess the cardioprotective effects of STS. Apoptosis of cells was measured with Hoechst 33342-based fluorescence microscopy, and annexin V-FITC-based flow cytometry. Caspase-3 and caspase-8 activities and mitochondrial membrane potential were also measured using commercial kits. TNF-α in the cell culture supernatant fractions were measured with sandwich elisa, and protein levels assayed using Western blot. Key Results STS inhibited OGD/R-induced apoptosis by suppressing JNK-mediated activation of NF-κB, TNF-α expression, activation of caspase-3 and caspase-8 and the Bax/Bcl-2 ratio. Additionally, positive feedback between NF-κB and TNF-α and amplification of TNF-α were inhibited, suggesting that STS plays a protective role against apoptosis in cardiomyocytes, even upon activation of pro-inflammatory cytokines. Interestingly, the cytoprotective effects of STS on OGD/R-induced apoptosis and promotion of cell survival were attenuated after inhibition of PI3K. Conclusion and Implications The inhibitory effects of STS on TNF-α and positive feedback signalling of the NF-κB/TNF-α pathways may play important roles in myocardial protection against ischaemia/reperfusion. These protective effects of STS are mediated by suppressing JNK activity through activation of the PI3K-Akt pathway. PMID:23517194

Hyperoxia Inhibits T Cell Activation in Mice

NASA Astrophysics Data System (ADS)

Hughes-Fulford, M.; Meissler, J.; Aguayo, E. T.; Globus, R.; Aguado, J.; Candelario, T.

2013-02-01

Background: The immune response is blunted in mice and humans in spaceflight. The effects of hyperoxia in mice alter expression of some of the same immune response genes. If these two conditions are additive, there could be an increased risk of infection in long duration missions. Immunosuppression is seen in healthy astronauts who have flown in space; however little is known about the mechanisms that cause the reduced immunity in spaceflight. Here we examine the role of oxidative stress on mice exposed to periods of high O2 levels mimicking pre-breathing protocols and extravehicular activity (EVA). To prevent decompression sickness, astronauts are exposed to elevated oxygen (hyperoxia) before and during EVA activities. Spaceflight missions may entail up to 24 hours of EVA per crewmember per week to perform construction and maintenance tasks. The effectiveness and success of these missions depends on designing EVA systems and protocols that maximize human performance and efficiency while minimizing health and safety risks for crewmembers. To our knowledge, no studies have been conducted on the immune system under 100% oxygen exposures to determine the potential for immune compromise due to prolonged and repeated EVAs. Methods: Animals were exposed to hyperoxic or control conditions for 8 hours per day over a period of 3 days, initiated 4 hours into the dark cycle (12h dark/12h light), using animal environmental control cabinets and oxygen controller (Biospherix, Lacona, NY). Experimental mice were exposed to 98-100% oxygen as a model for pre-breathing and EVA conditions, while control mice were maintained in chambers supplied with compressed air. These are ground control studies where we use real-time RTPCR (qRTPCR) to measure gene expression of the early immune gene expression during bead activation of splenocytes of normoxic and hyperoxic mice. All procedures were reviewed and approved by the IACUC at Ames Research Center. After the last 8h of hyperoxic exposure, spleens were removed and the splenocytes were isolated and kept as individual biological samples. We have also examined transcription factors (JASPAR) and pathways of the immune system to help us understand the mechanism of regulation. Results: Our recent mouse immunology experiment aboard STS-131 suggests that the early T cell immune response was inhibited in animals that have been exposed to spaceflight, even 24 hours after return to earth. Moreover, recent experiments in hyperoxic mice show that many of the same genes involved in early T cell activation were altered. Specifically, expression of IL-2Rα, Cxcl2, TNFα, FGF2, LTA and BCL2 genes are dysregulated in mice exposed to hyperoxia. Conclusions: If these hyperoxia-induced changes of gene expression in early T cell activation are additive to the changes seen in the microgravity of spaceflight, there could be an increased infection risk to EVA astronauts, which should be addressed prior to conducting a Mars or other long-term mission.

Molecular characterization and antibiotic resistance of clinical Streptococcus dysgalactiae subsp. equisimilis in Beijing, China.

PubMed

Lu, Binghuai; Fang, Yujie; Huang, Lei; Diao, Baowei; Du, Xiaoli; Kan, Biao; Cui, Yanchao; Zhu, Fengxia; Li, Dong; Wang, Duochun

2016-06-01

Streptococcus dysgalactiae subsp. equisimilis (SDSE) is presently considered as a human pathogen associated with clinical infection. We characterized 56 SDSE isolates collected from two tertiary hospitals in Beijing, China. Sixteen distinct emm types/subtypes were detected, dominated by stG245.0 (32.1%), stG652.0 (10.7%), stG6.1 (10.7%) and stG485.0 (10.7%), and a novel stG840.0 variant type was identified. All isolates possessed virulence genes of sagA and scpA, and most carried slo (98.2%), ska (98.2%) and speG(dys) (35.7%). By multilocus sequence typing (MLST) analysis, 17 individual sequence types (STs) were distinguished, including 7 newly-identified STs (26.8% of isolates), of which ST127 (30.4%), ST7 (12.5%) and ST44 (10.7%) dominated. Meanwhile, pulsed-field gel electrophoresis (PFGE) analysis revealed 33 pattern types (PTs), which were further combined into 16 pattern clusters (PCs), and 59.3% of isolates were distributed into 2 dominant PCs. Notably, emm types had both close relationship and consistency with STs and PFGE PCs. Furthermore, of 56 SDSE isolates, the predominant antibiotic resistances were erythromycin (71.4%), clindamycin (71.4%) and tetracycline (60.7%). Correspondingly, the prevalent resistance genes of macrolide and tetracycline were erm(B) (78.6%) and tet(M) (73.2%). In addition, multiple point mutations of parC, one of fluoroquinolone resistance genes, were observed (accounting for 75%), and were divided into 12 types, with parC 07 as the predominant type. Our data suggested the wide molecular diversity and distinctive regional features of SDSE from clinical infection in Beijing, China. Copyright © 2016 Elsevier B.V. All rights reserved.

Preparation and operation of space-based experiment on plant growth in KIBO, named Space Seed

NASA Astrophysics Data System (ADS)

Yano, Sachiko; Tanigaki, Fumiaki; Shimazu, Toru; Kasahara, Haruo; Nakamura, Tai; Karahara, Ichirou; Hoson, Takayuki; Kamisaka, Seiichiro

The Japan Aerospace Exploration Agency (JAXA) recently carried out plant growth experiment using the Plant Experiment Unit (PEU) installed in the Cell Biology Experiment Facility (CBEF) onboard KIBO. This experiment named Space Seed was designed to investigate the effect of microgravity on plant growth, especially seed to seed life cycle. Space shuttle STS-128 (17A) carrying eight PEU's was launched to the International Space Station (ISS) on August 28, 2009. The experiment was started on September 10 and terminated on November 11, 2009. The control system of environment in PEU and CBEF worked successfully as planned. In KIBO, Arabidopsis seeds germinated, and bolting and flowering were observed in the PEU's. In the end of March, 2010, Arabidopsis plants harvested in Kibo will be recovered to Earth by the space shuttle mission STS-131(19A) and analyzed for their biological characteristics such as seed fertility, cell wall properties, and gene expression. In this presentation, we describe the outline of the Space Seed experiment. We also describe experimental data such as the control of temperature and humidity in PEUs and CBEF, the onboard operations by the ISS crew, the procedure by which the experiment was monitored from the ground, and brief information about seed germination and subsequent growth under microgravity condition in space. We also succeeded in comparing the results of plant growth in PEUs on onboard 1-G control (Centrifuge) with results in microgravity condition and in ground control.

Tracking inter-institutional spread of NDM and identification of a novel NDM-positive plasmid, pSg1-NDM, using next-generation sequencing approaches.

PubMed

Khong, Wei Xin; Marimuthu, Kalisvar; Teo, Jeanette; Ding, Yichen; Xia, Eryu; Lee, Jia Jun; Ong, Rick Twee-Hee; Venkatachalam, Indumathi; Cherng, Benjamin; Pada, Surinder Kaur; Choong, Weng Lam; Smitasin, Nares; Ooi, Say Tat; Deepak, Rama Narayana; Kurup, Asok; Fong, Raymond; Van La, My; Tan, Thean Yen; Koh, Tse Hsien; Lin, Raymond Tzer Pin; Tan, Eng Lee; Krishnan, Prabha Unny; Singh, Siddharth; Pitout, Johann D; Teo, Yik-Ying; Yang, Liang; Ng, Oon Tek

2016-11-01

Owing to gene transposition and plasmid conjugation, New Delhi metallo-β-lactamase (NDM) is typically identified among varied Enterobacteriaceae species and STs. We used WGS to characterize the chromosomal and plasmid molecular epidemiology of NDM transmission involving four institutions in Singapore. Thirty-three Enterobacteriaceae isolates (collection years 2010-14) were sequenced using short-read sequencing-by-synthesis and analysed. Long-read single molecule, real-time sequencing (SMRTS) was used to characterize genetically a novel plasmid pSg1-NDM carried on Klebsiella pneumoniae ST147. In 20 (61%) isolates, bla NDM was located on the pNDM-ECS01 plasmid in the background of multiple bacterial STs, including eight K. pneumoniae STs and five Escherichia coli STs. In six (18%) isolates, a novel bla NDM -positive plasmid, pSg1-NDM, was found only in K. pneumoniae ST147. The pSg1-NDM-K. pneumoniae ST147 clone (Sg1-NDM) was fully sequenced using SMRTS. pSg1-NDM, a 90 103 bp IncR plasmid, carried genes responsible for resistance to six classes of antimicrobials. A large portion of pSg1-NDM had no significant homology to any known plasmids in GenBank. pSg1-NDM had no conjugative transfer region. Combined chromosomal-plasmid phylogenetic analysis revealed five clusters of clonal bacterial NDM-positive plasmid transmission, of which two were inter-institution clusters. The largest inter-institution cluster involved six K. pneumoniae ST147-pSg1-NDM isolates. Fifteen patients were involved in transmission clusters, of which four had ward contact, six had hospital contact and five had an unknown transmission link. A combined sequencing-by-synthesis and SMRTS approach can determine effectively the transmission clusters of bla NDM and genetically characterize novel plasmids. Plasmid molecular epidemiology is important to understanding NDM spread as bla NDM -positive plasmids can conjugate extensively across species and STs. © The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Dissemination of IMP-6-producing Pseudomonas aeruginosa ST244 in multiple cities in China.

PubMed

Chen, Y; Sun, M; Wang, M; Lu, Y; Yan, Z

2014-07-01

Pseudomonas aeruginosa is an important opportunistic pathogen responsible for nosocomial infections and is currently reported to be a worldwide nosocomial menace. The aim of this study was to investigate the epidemiological traits and the distribution of metallo-β-lactamases (MBLs)-producing P. aeruginosa clinical isolates in ten cities in China between January 2010 and May 2012. Antimicrobial susceptibility was determined by disc diffusion assay and the minimum inhibitory concentrations (MICs) of imipenem and meropenem were also determined by the Etest according to Clinical and Laboratory Standards Institute (CLSI) guidelines. In addition, polymerase chain reaction (PCR) and DNA sequencing were applied to detect bla MBL genes, and their epidemiological relationships were investigated by multilocus sequence typing (MLST). Of 368 P. aeruginosa isolates, MLST analysis identified 138 sequence types (STs), including 122 known and 16 novel STs, and the most frequently detected clone was ST244, followed by ST235. Besides, our study revealed that 25 isolates carried the bla IMP-6 gene and three isolates carried the bla VIM-2 gene, and a probe specific for both genes could be hybridised to an ~1,125-kb fragment in all isolates. Interestingly, all of the bla IMP-6-producing isolates shared an identical ST, ST244, and exhibited a higher level of resistance to several antibiotics. Overall, these observations suggest that P. aeruginosa ST244 carrying the chromosomally located bla IMP-6 gene is widely disseminated in multiple cites in China.

KSC-2011-1786

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- In Orbiter Processing Facility-2 at NASA’s Kennedy Space Center in Florida, workers prepare space shuttle Endeavour for its move, or "rollover," from its hangar to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1787

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- In Orbiter Processing Facility-2 at NASA’s Kennedy Space Center in Florida, workers prepare space shuttle Endeavour for its move, or "rollover," from its hangar to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1788

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, workers accompany space shuttle Endeavour as it is backed out of Orbiter Processing Facility-2 for its move, "or rollover," to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1799

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, workers accompany space shuttle Endeavour as it is backed out of Orbiter Processing Facility-2 for its move, or "rollover," to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Frankie Martin

KSC-2011-1785

NASA Image and Video Library

2011-02-28

CAPE CANAVERAL, Fla. -- In Orbiter Processing Facility-2 at NASA’s Kennedy Space Center in Florida, workers prepare space shuttle Endeavour for its move, or "rollover," from its hangar to the Vehicle Assembly Building. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, spare parts, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2009-2241

NASA Image and Video Library

2009-03-21

CAPE CANAVERAL, Fla. – On the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, a worker removes a cover from the EXPRESS Logistics Carrier for the STS-129 mission. The truck and carrier arrived on the C-17 cargo plane in the background. The carrier is part of the payload on space shuttle Atlantis, which will deliver to the International Space Station components including two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Tim Jacobs

KSC-08pd3210

NASA Image and Video Library

2008-10-17

CAPE CANAVERAL, Fla. – This photo shows the crew galley that will be delivered to the International Space Station aboard space shuttle Endeavour on the STS-126 mission. It is designed to make use of the top half of EXPRESS Rack 6 for power, cooling and water. It will consist of one potable water dispenser, one food warmer, and one MERLIN refrigerator. Once on orbit, the galley will be transferred to the U.S. Lab. Endeavour and its crew of seven are scheduled to lift off at 7:55 p.m. Nov. 14 for the 15-day STS-126 mission. Photo credit: NASA

Comparative Genotypes, Staphylococcal Cassette Chromosome mec (SCCmec) Genes and Antimicrobial Resistance amongst Staphylococcus epidermidis and Staphylococcus haemolyticus Isolates from Infections in Humans and Companion Animals.

PubMed

McManus, Brenda A; Coleman, David C; Deasy, Emily C; Brennan, Gráinne I; O' Connell, Brian; Monecke, Stefan; Ehricht, Ralf; Leggett, Bernadette; Leonard, Nola; Shore, Anna C

2015-01-01

This study compares the characteristics of Staphylococcus epidermidis (SE) and Staphylococcus haemolyticus (SH) isolates from epidemiologically unrelated infections in humans (Hu) (28 SE-Hu; 8 SH-Hu) and companion animals (CpA) (12 SE-CpA; 13 SH-CpA). All isolates underwent antimicrobial susceptibility testing, multilocus sequence typing and DNA microarray profiling to detect antimicrobial resistance and SCCmec-associated genes. All methicillin-resistant (MR) isolates (33/40 SE, 20/21 SH) underwent dru and mecA allele typing. Isolates were predominantly assigned to sequence types (STs) within a single clonal complex (CC2, SE, 84.8%; CC1, SH, 95.2%). SCCmec IV predominated among MRSE with ST2-MRSE-IVc common to both Hu (40.9%) and CpA (54.5%). Identical mecA alleles and nontypeable dru types (dts) were identified in one ST2-MRSE-IVc Hu and CpA isolate, however, all mecA alleles and 2/4 dts detected among 18 ST2-MRSE-IVc isolates were closely related, sharing >96.5% DNA sequence homology. Although only one ST-SCCmec type combination (ST1 with a non-typeable [NT] SCCmec NT9 [class C mec and ccrB4]) was common to four MRSH-Hu and one MRSH-CpA, all MRSH isolates were closely related based on similar STs, SCCmec genes (V/VT or components thereof), mecA alleles and dts. Overall, 39.6% of MR isolates harbored NT SCCmec elements, and ACME was more common amongst MRSE and CpA isolates. Multidrug resistance (MDR) was detected among 96.7% of isolates but they differed in the prevalence of specific macrolide, aminoglycoside and trimethoprim resistance genes amongst SE and SH isolates. Ciprofloxacin, rifampicin, chloramphenicol [fexA, cat-pC221], tetracycline [tet(K)], aminoglycosides [aadD, aphA3] and fusidic acid [fusB] resistance was significantly more common amongst CpA isolates. SE and SH isolates causing infections in Hu and CpA hosts belong predominantly to STs within a single lineage, harboring similar but variable SCCmec genes, mecA alleles and dts. Host and staphylococcal species-specific characteristics were identified in relation to antimicrobial resistance genes and phenotypes, SCCmec and ACME.

Comparative Genotypes, Staphylococcal Cassette Chromosome mec (SCCmec) Genes and Antimicrobial Resistance amongst Staphylococcus epidermidis and Staphylococcus haemolyticus Isolates from Infections in Humans and Companion Animals

PubMed Central

McManus, Brenda A.; Coleman, David C.; Deasy, Emily C.; Brennan, Gráinne I.; O’ Connell, Brian; Monecke, Stefan; Ehricht, Ralf; Leggett, Bernadette; Leonard, Nola; Shore, Anna C.

2015-01-01

This study compares the characteristics of Staphylococcus epidermidis (SE) and Staphylococcus haemolyticus (SH) isolates from epidemiologically unrelated infections in humans (Hu) (28 SE-Hu; 8 SH-Hu) and companion animals (CpA) (12 SE-CpA; 13 SH-CpA). All isolates underwent antimicrobial susceptibility testing, multilocus sequence typing and DNA microarray profiling to detect antimicrobial resistance and SCCmec-associated genes. All methicillin-resistant (MR) isolates (33/40 SE, 20/21 SH) underwent dru and mecA allele typing. Isolates were predominantly assigned to sequence types (STs) within a single clonal complex (CC2, SE, 84.8%; CC1, SH, 95.2%). SCCmec IV predominated among MRSE with ST2-MRSE-IVc common to both Hu (40.9%) and CpA (54.5%). Identical mecA alleles and nontypeable dru types (dts) were identified in one ST2-MRSE-IVc Hu and CpA isolate, however, all mecA alleles and 2/4 dts detected among 18 ST2-MRSE-IVc isolates were closely related, sharing >96.5% DNA sequence homology. Although only one ST-SCCmec type combination (ST1 with a non-typeable [NT] SCCmec NT9 [class C mec and ccrB4]) was common to four MRSH-Hu and one MRSH-CpA, all MRSH isolates were closely related based on similar STs, SCCmec genes (V/VT or components thereof), mecA alleles and dts. Overall, 39.6% of MR isolates harbored NT SCCmec elements, and ACME was more common amongst MRSE and CpA isolates. Multidrug resistance (MDR) was detected among 96.7% of isolates but they differed in the prevalence of specific macrolide, aminoglycoside and trimethoprim resistance genes amongst SE and SH isolates. Ciprofloxacin, rifampicin, chloramphenicol [fexA, cat-pC221], tetracycline [tet(K)], aminoglycosides [aadD, aphA3] and fusidic acid [fusB] resistance was significantly more common amongst CpA isolates. SE and SH isolates causing infections in Hu and CpA hosts belong predominantly to STs within a single lineage, harboring similar but variable SCCmec genes, mecA alleles and dts. Host and staphylococcal species-specific characteristics were identified in relation to antimicrobial resistance genes and phenotypes, SCCmec and ACME. PMID:26379051

KSC-2011-3277

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, technicians remove the cover on the Load Control Assembly-2 (LCA-2) to begin the testing process. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3284

NASA Image and Video Library

2011-05-04

CAPE CANAVERAL, Fla. -- At NASA Kennedy Space Center's Launch Pad 39A, the Load Control Assembly-2 (LCA-2) has been replaced inside of space shuttle Endeavour. Located in Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission and has been replaced. Systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

KSC-2011-3280

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, technicians begin the testing process on the Load Control Assembly-2 (LCA-2) after the cover has been removed. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3285

NASA Image and Video Library

2011-05-04

CAPE CANAVERAL, Fla. -- At NASA Kennedy Space Center's Launch Pad 39A, the Load Control Assembly-2 (LCA-2) has been replaced inside of space shuttle Endeavour. Located in Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission and has been replaced. Systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

KSC-2011-3282

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, the Load Control Assembly-2 (LCA-2) is uncovered for testing. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3286

NASA Image and Video Library

2011-05-04

CAPE CANAVERAL, Fla. -- At NASA Kennedy Space Center's Launch Pad 39A, the Load Control Assembly-2 (LCA-2) has been replaced inside of space shuttle Endeavour. Located in Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission and has been replaced. Systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

KSC-2011-3287

NASA Image and Video Library

2011-05-04

CAPE CANAVERAL, Fla. -- At NASA Kennedy Space Center's Launch Pad 39A, the access door is open on space shuttle Endeavour for technicians to enter the aft area where the Load Control Assembly-2 (LCA-2) is located. Located in Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission and has been replaced. Systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

KSC-2011-3283

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, the Load Control Assembly-2 (LCA-2) is uncovered for testing. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3281

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, the Load Control Assembly-2 (LCA-2) is uncovered for testing. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3289

NASA Image and Video Library

2011-05-04

CAPE CANAVERAL, Fla. -- At NASA Kennedy Space Center's Launch Pad 39A, space shuttle Endeavour sits poised for launch after technicians replaced the Load Control Assembly-2 (LCA-2) in its aft section. Located in Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission and has been replaced. Systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

KSC-2011-3279

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, technicians begin the testing process on the Load Control Assembly-2 (LCA-2) after the cover has been removed. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3276

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, technicians remove the cover on the Load Control Assembly-2 (LCA-2) to begin the testing process. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3278

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, technicians begin the testing process on the Load Control Assembly-2 (LCA-2) after the cover has been removed. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-3288

NASA Image and Video Library

2011-05-04

CAPE CANAVERAL, Fla. -- At NASA Kennedy Space Center's Launch Pad 39A, space shuttle Endeavour sits poised for launch after technicians replaced the Load Control Assembly-2 (LCA-2) in its aft section. Located in Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission and has been replaced. Systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

KSC-2011-3275

NASA Image and Video Library

2011-05-03

CAPE CANAVERAL, Fla. -- At the NASA Shuttle Logistics Depot in Cape Canaveral, Florida, technicians carefully remove the Load Control Assembly-2 (LCA-2) from a cart for testing. Located in space shuttle Endeavour's aft avionics bay 5, the LCA-2, which distributes power to nine shuttle systems, is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt for the STS-134 mission. The LCA-2 will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-4200

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Workers measured and marked the spot where shuttle Endeavour's nose gear came to a stop after the vehicle's final return from space completing a 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4173

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Astronaut Rick Sturckow flies weather reconnaissance in a Shuttle Training Aircraft over NASA's Kennedy Space Center in Florida to assess conditions before space shuttle Endeavour returns to Earth for the final time. Weather was observed "go" and Endeavour glided to a stop on the Shuttle Landing Facility's Runway 15 at 2:35 a.m. EDT, bringing an end to the STS-134 mission. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Tony Gray

KSC-2011-3740

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- In the Press Site auditorium at NASA's Kennedy Space Center in Florida, staff for U.S. Congresswoman Gabielle Giffords brief media after the successful launch of space shuttle Endeavour on its STS-134 mission. From left are, Pia Carusone Chief of Staff, U.S. Rep. Gabrielle Giffords Office, Mark Kimble Press Advisor, U.S. Rep. Gabrielle Giffords Office and Ashley Nash-Hahn, New Media Strategist, U.S. Rep. Gabrielle Giffords Office. Giffords was on hand to watch her husband, STS-135 Commander Mark Kelly and his crew liftoff from Launch Pad 39A on Endeavour's final spaceflight. Endeavour lifted off May 16 at 8:56 a.m. EDT. Endeavour and its crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the station. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

Molecular markers shared by diverse apomictic Pennisetum species.

PubMed

Lubbers, E L; Arthur, L; Hanna, W W; Ozias-Akins, P

1994-11-01

Two molecular markers, a RAPD (randomly amplified polymorphic DNA) and a RFLP/STS (restriction fragment length polymorphism/sequence-tagged site), previously were found associated with apomictic reproductive behavior in a backcross population produced to transfer apomixis from Pennisetum squamulatum to pearl millet. The occurrence of these molecular markers in a range of 29 accessions of Pennisetum comprising 11 apomictic and 8 sexual species was investigated. Both markers were specific for apomictic species in Pennisetum. The RFLP/STS marker, UGT 197, was found to be associated with all taxa that displayed apomictic reproductive behavior except those in section Brevivalvula. Neither UGT197 nor the cloned RAPD fragment OPC-04600 hybridized with any sexually reproducing representatives of the genus. The cloned C04600 was associated with 3 of the 11 apomictic species, P. ciliare, P. massaicum, and P. squamulatum. UGT197 was more consistently associated with apomictic reproductive behavior than OPC04600 or cloned C04600, thus it could be inferred that UGT197 is more closely linked to the gene(s) for apomixis than the cloned C04600. The successful use of these probes to survey other Pennisetum species indicates that apomixis is a trait that can be followed across species by using molecular means. This technique of surveying species within a genus will be useful in determining the relative importance of newly isolated markers and may facilitate the identification of the apomixis gene(s).

Event-related repetitive TMS reveals distinct, critical roles for right OFA and bilateral posterior STS in judging the sex and trustworthiness of faces.

PubMed

Dzhelyova, Milena P; Ellison, Amanda; Atkinson, Anthony P

2011-10-01

Judging the sex of faces relies on cues related to facial morphology and spatial relations between features, whereas judging the trustworthiness of faces relies on both structural and expressive cues that signal affective valence. The right occipital face area (OFA) processes structural cues and has been associated with sex judgments, whereas the posterior STS processes changeable facial cues related to muscle movements and is activated when observers judge trustworthiness. It is commonly supposed that the STS receives inputs from the OFA, yet it is unknown whether these regions have functionally dissociable, critical roles in sex and trustworthiness judgments. We addressed this issue using event-related, fMRI-guided repetitive transcranial magnetic stimulation (rTMS). Twelve healthy volunteers judged the sex of individually presented faces and, in a separate session, whether those same faces were trustworthy or not. Relative to sham stimulation, RTs were significantly longer for sex judgments when rTMS was delivered over the right OFA but not the right or left STS, and for trustworthiness judgments on male but not female faces when rTMS was delivered over the right STS or left STS but not the right OFA. Nonetheless, an analysis of the RT distributions revealed a possible critical role also for the right OFA in trustworthiness judgments, limited to faces with longer RTs, perhaps reflecting the later, ancillary use of structural cues related to the sex of the face. On the whole, our findings provide evidence that evaluations of the trustworthiness and sex of faces rely on functionally dissociable cortical regions.

[Shereshevsky-Turner syndrome: Estrogen replacement therapy and cardiovascular risk factors].

PubMed

Yevstigneeva, O A; Andreeva, E N; Grigoryan, O R; Volevodz, N N; Melnichenko, G A; Dedov, I I

To investigate the impact of menopausal hormone therapy (MHT) on the expression of risk factors for cardiovascular events (CVEs) in patients with Shereshevsky-Turner syndrome (STS); to elaborate an algorithm for patient management using MHT. From 2010 to 2012, a total of 41 patients aged 14 to 35 years with STS were examined in the framework of a prospective observational study. 100 STS case histories in 2000 to 2009 were retrospectively analyzed. The indicators of the so-called cardiometabolic risk, such as body mass index (BMI), lipidogram readings, venous plasma glucose levels, and blood pressure, were estimated in relation to the type of MHT. In the prospective part of the investigation, an angioscan was used to estimate vessel characteristics (stiffness, wall tone, endothelial function (EF)), by using the examination data. 90% of the patients with STS were found to have risk factors for CVEs: atherogenic dyslipidemia (85%; 51% in the general female population of the same age), diastolic hypertension (36%; no more than 5% that is not typical for age-matched healthy general female population). In addition to increased arterial wall stiffness (AWS), obvious EF disorder is typical for STS patients. MHT was accompanied by a dose-dependent (estradiol, at least 2 mg) reduction in diastolic blood pressure by an average of 13% over 24 months, an increase in high density lipoprotein levels by more than 10% over 24 months and also contributedto a decrease in AWS and an improvement in EF. By favorably affecting the EF of vessels and reducing the severity of atherogenic dyslipidemia, MHT potentially enables a reduction in CV risk in patients with STS.

Population Structure of Clinical Pseudomonas aeruginosa from West and Central African Countries

PubMed Central

Cholley, Pascal; Ka, Roughyatou; Guyeux, Christophe; Thouverez, Michelle; Guessennd, Nathalie; Ghebremedhin, Beniam; Frank, Thierry; Bertrand, Xavier; Hocquet, Didier

2014-01-01

Background Pseudomonas aeruginosa (PA) has a non-clonal, epidemic population with a few widely distributed and frequently encountered sequence types (STs) called ‘high-risk clusters’. Clinical P. aeruginosa (clinPA) has been studied in all inhabited continents excepted in Africa, where a very few isolates have been analyzed. Here, we characterized a collection of clinPA isolates from four countries of West and Central Africa. Methodology 184 non-redundant isolates of clinPA from hospitals of Senegal, Ivory Coast, Nigeria, and Central African Republic were genotyped by MLST. We assessed their resistance level to antibiotics by agar diffusion and identified the extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs) by sequencing. The population structure of the species was determined by a nucleotide-based analysis of the entire PA MLST database and further localized on the phylogenetic tree (i) the sequence types (STs) of the present collection, (ii) the STs by continents, (iii) ESBL- and MBL-producing STs from the MLST database. Principal Findings We found 80 distinct STs, of which 24 had no relationship with any known STs. ‘High-risk’ international clonal complexes (CC155, CC244, CC235) were frequently found in West and Central Africa. The five VIM-2-producing isolates belonged to CC233 and CC244. GES-1 and GES-9 enzymes were produced by one CC235 and one ST1469 isolate, respectively. We showed the spread of ‘high-risk’ international clonal complexes, often described as multidrug-resistant on other continents, with a fully susceptible phenotype. The MBL- and ESBL-producing STs were scattered throughout the phylogenetic tree and our data suggest a poor association between a continent and a specific phylogroup. Conclusions ESBL- and MBL-encoding genes are borne by both successful international clonal complexes and distinct local STs in clinPA of West and Central Africa. Furthermore, our data suggest that the spread of a ST could be either due to its antibiotic resistance or to features independent from the resistance to antibiotics. PMID:25187957

KSC-2009-6403

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Media representatives and Twitter followers participate in a post-launch news conference in the NASA Press Site auditorium at NASA's Kennedy Space Center in Florida after the successful launch of space shuttle Atlantis. On the dais, from left, are Public Affairs moderator Mike Curie; Bill Gerstenmaier, associate administrator for Space Operations; Mike Moses, chair, Mission Management Team; and Mike Leinbach, space shuttle launch director. Liftoff of Atlantis on its STS-129 mission came at 2:28 p.m. EST Nov. 16 from Launch Pad 39A. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

STS-129 Tweetup

NASA Image and Video Library

2009-11-16

NASA Twitter followers attending a two-day NASA Tweetup presented NASA Public Affairs Officers with a signed poster from Kennedy Space Center expressing their appreciation for hosting this unique event, Monday, Nov. 16, 2009 in Cape Canaveral, Fla. Photo Credit: (NASA/Carla Cioffi)

Chondroitin 6-O-sulfotransferases are required for morphogenesis of the notochord in the ascidian embryo.

PubMed

Nakamura, Jun; Yoshida, Keita; Sasakura, Yasunori; Fujiwara, Shigeki

2014-12-01

Chondroitin sulfate (CS) is a sulfated polysaccharide chain that binds to various core proteins to form proteoglycans. The amount and position of sulfate groups in CS are variable among different tissues, and are determined by specific sulfotransferases. Although the ascidians are the closest relatives of vertebrates, the functions of their sulfotransferases have not been studied. The genome of the ascidian Ciona intestinalis contains eight genes encoding proteins similar to chondroitin 6-O-sulfotransferases (C6STs), which appear to have independently diverged in the ascidian lineage during evolution. Among them, Ci-C6ST-like1 and Ci-C6ST-like7 were predominantly expressed in the developing notochord. In addition, they were weakly expressed in the neural tube. The disruption of either one of them affected the convergent extension movement of notochordal cells. Presumptive notochord cells coming from both sides of the embryo did not intercalate. The results suggest that both of them are necessary. In some cases, the anterior neural tube failed to close. Forced expression of Ci-C6ST-like1 or Ci-C6ST-like7 in the notochord restored the normal intercalation of notochordal cells, indicating that the effects of morpholino oligos are specific. Ci-C6ST-like1 and Ci-C6ST-like7 are required for the morphogenesis of the notochord in the ascidian embryo. © 2014 Wiley Periodicals, Inc.

Infarct-Induced Steroidogenic Acute Regulatory Protein: A Survival Role in Cardiac Fibroblasts

PubMed Central

Anuka, Eli; Yivgi-Ohana, Natalie; Eimerl, Sarah; Garfinkel, Benjamin; Melamed-Book, Naomi; Chepurkol, Elena; Aravot, Dan; Zinman, Tova; Shainberg, Asher; Hochhauser, Edith

2013-01-01

Steroidogenic acute regulatory protein (StAR) is indispensable for steroid hormone synthesis in the adrenal cortex and the gonadal tissues. This study reveals that StAR is also expressed at high levels in nonsteroidogenic cardiac fibroblasts confined to the left ventricle of mouse heart examined 3 days after permanent ligation of the left anterior descending coronary artery. Unlike StAR, CYP11A1 and 3β-hydroxysteroid dehydrogenase proteins were not observed in the postinfarction heart, suggesting an apparent lack of de novo cardiac steroidogenesis. Work with primary cultures of rat heart cells revealed that StAR is induced in fibroblasts responding to proapoptotic treatments with hydrogen peroxide or the kinase inhibitor staurosporine (STS). Such induction of StAR in culture was noted before spontaneous differentiation of the fibroblasts to myofibroblasts. STS induction of StAR in the cardiac fibroblasts conferred a marked resistance to apoptotic cell death. Consistent with that finding, down-regulation of StAR by RNA interference proportionally increased the number of STS-treated apoptotic cells. StAR down-regulation also resulted in a marked increase of BAX activation in the mitochondria, an event known to associate with the onset of apoptosis. Last, STS treatment of HeLa cells showed that apoptotic demise characterized by mitochondrial fission, cytochrome c release, and nuclear fragmentation is arrested in individual HeLa cells overexpressing StAR. Collectively, our in vivo and ex vivo evidence suggests that postinfarction expression of nonsteroidogenic StAR in cardiac fibroblasts has novel antiapoptotic activity, allowing myofibroblast precursor cells to survive the traumatized event, probably to differentiate and function in tissue repair at the infarction site. PMID:23831818

Imaging Primary Mouse Sarcomas After Radiation Therapy Using Cathepsin-Activatable Fluorescent Imaging Agents

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cuneo, Kyle C.; Mito, Jeffrey K.; Javid, Melodi P.

2013-05-01

Purpose: Cathepsin-activated fluorescent probes can detect tumors in mice and in canine patients. We previously showed that these probes can detect microscopic residual sarcoma in the tumor bed of mice during gross total resection. Many patients with soft tissue sarcoma (STS) and other tumors undergo radiation therapy (RT) before surgery. This study assesses the effect of RT on the ability of cathepsin-activated probes to differentiate between normal and cancerous tissue. Methods and Materials: A genetically engineered mouse model of STS was used to generate primary hind limb sarcomas that were treated with hypofractionated RT. Mice were injected intravenously with cathepsin-activatedmore » fluorescent probes, and various tissues, including the tumor, were imaged using a hand-held imaging device. Resected tumor and normal muscle samples were harvested to assess cathepsin expression by Western blot. Uptake of activated probe was analyzed by flow cytometry and confocal microscopy. Parallel in vitro studies using mouse sarcoma cells were performed. Results: RT of primary STS in mice and mouse sarcoma cell lines caused no change in probe activation or cathepsin protease expression. Increasing radiation dose resulted in an upward trend in probe activation. Flow cytometry and immunofluorescence showed that a substantial proportion of probe-labeled cells were CD11b-positive tumor-associated immune cells. Conclusions: In this primary murine model of STS, RT did not affect the ability of cathepsin-activated probes to differentiate between tumor and normal muscle. Cathepsin-activated probes labeled tumor cells and tumor-associated macrophages. Our results suggest that it would be feasible to include patients who have received preoperative RT in clinical studies evaluating cathepsin-activated imaging probes.« less

KSC-05pd2357

NASA Image and Video Library

2005-11-01

KENNEDY SPACE CENTER, FLA. - In NASA’s Orbiter Processing Facility Bay 3, United Space Alliance technicians Gene Peavler (left) and Richard McGehee (right) are on a stand removing gap filler and inspecting tile repair on Discovery’s underside. Discovery processing is under way for the second return to flight test mission, STS-121.

KSC-2011-3574

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour's six STS-134 astronauts, dressed in their orange launch-and-entry suits, wave to news media and other spectators in front of the Astrovan parked in front of the Operations and Checkout Building at NASA's Kennedy Space Center in Florida. The modified Airstream recreational vehicle, which has transported astronauts to their spacecraft since 1984, will take the crew to Launch Pad 39A. From left, are Mission Specialists Greg Chamitoff, Andrew Feustel, Roberto Vittori with the European Space Agency and Michael Fincke, Pilot Greg H. Johnson, and Commander Mark Kelly. STS-134 will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. May 16 at 8:56 a.m. will be the second launch attempt for Endeavour. The first attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

UniGene Tabulator: a full parser for the UniGene format.

PubMed

Lenzi, Luca; Frabetti, Flavia; Facchin, Federica; Casadei, Raffaella; Vitale, Lorenza; Canaider, Silvia; Carinci, Paolo; Zannotti, Maria; Strippoli, Pierluigi

2006-10-15

UniGene Tabulator 1.0 provides a solution for full parsing of UniGene flat file format; it implements a structured graphical representation of each data field present in UniGene following import into a common database managing system usable in a personal computer. This database includes related tables for sequence, protein similarity, sequence-tagged site (STS) and transcript map interval (TXMAP) data, plus a summary table where each record represents a UniGene cluster. UniGene Tabulator enables full local management of UniGene data, allowing parsing, querying, indexing, retrieving, exporting and analysis of UniGene data in a relational database form, usable on Macintosh (OS X 10.3.9 or later) and Windows (2000, with service pack 4, XP, with service pack 2 or later) operating systems-based computers. The current release, including both the FileMaker runtime applications, is freely available at http://apollo11.isto.unibo.it/software/

KSC-2011-1977

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is being lowered into a high bay to be attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-1991

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is lowered into place where it is being attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-1990

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is lowered into place where it is being attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-1981

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is lowered into place where it will be attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-1978

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is being lowered into place where it will be attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-1976

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is poised above a high bay where it will be lowered and attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-1993

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is lowered into place where it is being attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-1992

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is lowered into place where it is being attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-1980

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is lowered into place where it will be attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-1979

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is lowered into place where it will be attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

KSC-2009-5129

NASA Image and Video Library

2009-09-15

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, a crane moves the plasma contactor unit, or PCU, that will be installed on the Express Logistics Carrier, or ELC. The PCU is used to disperse electrical charge build-ups on the International Space Station. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jack Pfaller

KSC-2009-5128

NASA Image and Video Library

2009-09-15

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, a crane moves the plasma contactor unit, or PCU, that will be installed on the Express Logistics Carrier, or ELC. The PCU is used to disperse electrical charge build-ups on the International Space Station. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jack Pfaller

KSC-2009-5130

NASA Image and Video Library

2009-09-15

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, a crane lowers the plasma contactor unit, or PCU, that will be installed on the Express Logistics Carrier, or ELC. The PCU is used to disperse electrical charge build-ups on the International Space Station. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jack Pfaller

KSC-2009-5127

NASA Image and Video Library

2009-09-15

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, a crane lifts the plasma contactor unit, or PCU, that will be installed on the Express Logistics Carrier, or ELC. The PCU is used to disperse electrical charge build-ups on the International Space Station. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jack Pfaller

KSC-2009-5131

NASA Image and Video Library

2009-09-15

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, a crane lowers the plasma contactor unit, or PCU, that will be installed on the Express Logistics Carrier, or ELC. The PCU is used to disperse electrical charge build-ups on the International Space Station. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jack Pfaller

KSC-2009-5126

NASA Image and Video Library

2009-09-15

CAPE CANAVERAL, Fla. – In the Space Station Processing Facility at NASA's Kennedy Space Center in Florida, a crane lifts the plasma contactor unit, or PCU, that will be installed on the Express Logistics Carrier, or ELC. The PCU is used to disperse electrical charge build-ups on the International Space Station. The carrier is part of the STS-129 payload on space shuttle Atlantis, which will deliver to the International Space Station two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Jack Pfaller

STS-112 Crew Interviews: Yurchikhin

NASA Technical Reports Server (NTRS)

2002-01-01

A preflight interview with mission specialist Fyodor Yurchikhin is presented. He worked for a long time in Energia in the Russian Mission Control Center (MCC). Yurchikhin discusses the main goal of the STS-112 flight, which is to install the Integrated Truss Assembly S1 (Starboard Side Thermal Radiator Truss) on the International Space Station. He also talks about the three space walks required to install the S1. After the installation of S1, work with the bolts and cameras are performed. Yurchikhin is involved in working with nitrogen and ammonia jumpers. He expresses the complexity of his work, but says that he and the other crew members are ready for the challenge.

STS-114: Discovery Crew Post Landing Press Briefing

NASA Technical Reports Server (NTRS)

2005-01-01

The crew of the STS-114 Discovery is shown during a post landing press briefing. Commander Collins introduces the crew members who consist of Pilot Jim Kelley, Mission Specialist Soichi Noguchi from JAXA, Steve Robinson, Mission Specialist and Charlie Camarda, Mission Specialist. Steve Robinson answers a question from the news media about the repair that he performed in orbit, and his feelings about being back in his hometown of California. Commander Collins talks about the most significant accomplishment of the mission. The briefing ends as each crewmember reflects on the Space Shuttle Columbia tragedy and expresses their personal thoughts and feelings as they re-entered the Earth's atmosphere.

KSC-2009-2238

NASA Image and Video Library

2009-03-21

CAPE CANAVERAL, Fla. – A C-17 cargo plane arrives at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida with its cargo of the EXPRESS Logistics Carrier for the STS-129 mission. In the background is the mate/demate device used to separate a space shuttle from the Shuttle Carrier Aircraft. The carrier is part of the payload on space shuttle Atlantis, which will deliver to the International Space Station components including two spare gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. STS-129 is targeted to launch Nov. 12. Photo credit: NASA/Tim Jacobs

Genotyping of Salmonella enterica serovar Typhi strains isolated from 1959 to 2006 in China and analysis of genetic diversity by genomic microarray.

PubMed

Zhang, Haifang; Zhang, Xiaolei; Yan, Meiying; Pang, Bo; Kan, Biao; Xu, Huaxi; Huang, Xinxiang

2011-12-15

To determine the genotype of Salmonella enterica serovar Typhi (S. Typhi) strains in China and analyze their genetic diversity. We collected S. Typhi strains from 1959 to 2006 in five highly endemic Chinese provinces and chose 40 representative strains. Multilocus sequence typing was used to determine the genotypes or sequence types (ST) and microarray-based comparative genomic hybridization (M-CGH) to investigate the differences in gene content among these strains. Forty representative S. Typhi strains belonged to 4 sequence types (ST1, ST2, ST890, and ST892). The predominant S. Typhi genotype (31/40) was ST2 and it had a diverse geographic distribution. We discovered two novel STs - ST890 and ST892. M-CGH showed that 69 genes in these two novel STs were divergent from S. Typhi Ty2, which belongs to ST1. In addition, 5 representative Typhi strains of ST2 isolated from Guizhou province showed differences in divergent genes. We determined two novel sequence types, ST890 and ST892, and found that ST2 was the most prevalent genotype of S. Typhi in China. Genetic diversity was present even within a highly clonal bacterial population.

Gene flow at major transitional areas in sea bass (Dicentrarchus labrax) and the possible emergence of a hybrid swarm

PubMed Central

Quéré, Nolwenn; Desmarais, Erick; Tsigenopoulos, Costas S; Belkhir, Khalid; Bonhomme, François; Guinand, Bruno

2012-01-01

The population genetic structure of sea bass (Dicentrarchus labrax) along a transect from the Atlantic Ocean (AO) to the Eastern Mediterranean (EM) Sea differs from that of most other marine taxa in this area. Three populations (AO, Western Mediterranean [WM], EM) are recognized today, which were originally two allopatric populations. How two ancestral genetic units have evolved into three distinct units has not been addressed yet. Therefore, to investigate mechanisms that lead to the emergence of the central WM population, its current status, and its connectivity with the two parental populations, we applied 20 nuclear loci that were either gene associated or gene independent. Results confirmed the existence of three distinct gene pools, with higher differentiation at two transitional areas, the Almeria-Oran Front (AOF) and of the Siculo-Tunisian Strait (STS), than within any population. Significant linkage disequilibrium and heterozygote excess indicated that the STS is probably another tension zone, as already described for the AOF. Neutrality tests fail to reveal marker loci that could be driven by selection within or among metapopulations, except for locus DLA0068. Collectively, results support that the central WM population arose by trapping two tensions zones at distinct geographic locations of limited connectivity. Population assignment further revealed that WM individuals were more introgressed than individuals from the other two metapopulations. This suggests that this population might result from hybrid swarming, and was or is still seeded by genes received through the filter of each tension zone. PMID:23301173

Millie Hughes-Fulford, Scientist and Prior Astronaut

NASA Image and Video Library

2014-03-13

CAPE CANAVERAL, Fla. - T-cell science team member Miya Yoshida, of the Hughes-Fulford Laboratory in San Francisco, Calif., works in a biosafety hood during preflight experiment preparations in the Space Station Processing Facility at NASA's Kennedy Space Center in Florida. The immunology experiment will launch on SpaceX-3 and focus on the effects of microgravity on early T-cell signaling pathways. Current work aims to identify and compare the gene expression of microRNAs miRNAs during T-cell activation under normal gravity and in microgravity, and compare those patterns to changes seen in aging populations. The experiment will be the first flown on SpaceX funded by the National Institutes of Health. Dr. Hughes-Fulford flew aboard space shuttle mission STS-40 in June 1991, the first Spacelab mission dedicated to biomedical studies. For more information on the T-cell experiment, visit http://hughesfulfordlab.com and http://www.nasa.gov/ames/research/space-biosciences/t-cell-activation-in-aging-spacex-3/. Photo credit: NASA/Cory Huston

Millie Hughes-Fulford, Scientist and Prior Astronaut

NASA Image and Video Library

2014-03-13

CAPE CANAVERAL, Fla. - Researcher and principal investigator Dr. Millie Hughes-Fulford, of the Hughes-Fulford Laboratory, San Francisco, Calif., at the microscope, examines T-cells as part of preflight experiment operations in the Space Station Processing Facility at NASA's Kennedy Space Center in Florida. The immunology experiment will launch on SpaceX-3 and focus on the effects of microgravity on early T-cell signaling pathways. Current work aims to identify and compare the gene expression of microRNAs miRNAs during T-cell activation under normal gravity and in microgravity, and compare those patterns to changes seen in aging populations. The experiment will be the first flown on SpaceX funded by the National Institutes of Health. Dr. Hughes-Fulford flew aboard space shuttle mission STS-40 in June 1991, the first Spacelab mission dedicated to biomedical studies. For more information on the T-cell experiment, visit http://hughesfulfordlab.com and http://www.nasa.gov/ames/research/space-biosciences/t-cell-activation-in-aging-spacex-3/. Photo credit: NASA/Cory Huston

Science-Technology-Society literacy in college non-majors biology: Comparing problem/case studies based learning and traditional expository methods of instruction

NASA Astrophysics Data System (ADS)

Peters, John S.

This study used a multiple response model (MRM) on selected items from the Views on Science-Technology-Society (VOSTS) survey to examine science-technology-society (STS) literacy among college non-science majors' taught using Problem/Case Studies Based Learning (PBL/CSBL) and traditional expository methods of instruction. An initial pilot investigation of 15 VOSTS items produced a valid and reliable scoring model which can be used to quantitatively assess student literacy on a variety of STS topics deemed important for informed civic engagement in science related social and environmental issues. The new scoring model allows for the use of parametric inferential statistics to test hypotheses about factors influencing STS literacy. The follow-up cross-institutional study comparing teaching methods employed Hierarchical Linear Modeling (HLM) to model the efficiency and equitability of instructional methods on STS literacy. A cluster analysis was also used to compare pre and post course patterns of student views on the set of positions expressed within VOSTS items. HLM analysis revealed significantly higher instructional efficiency in the PBL/CSBL study group for 4 of the 35 STS attitude indices (characterization of media vs. school science; tentativeness of scientific models; cultural influences on scientific research), and more equitable effects of traditional instruction on one attitude index (interdependence of science and technology). Cluster analysis revealed generally stable patterns of pre to post course views across study groups, but also revealed possible teaching method effects on the relationship between the views expressed within VOSTS items with respect to (1) interdependency of science and technology; (2) anti-technology; (3) socioscientific decision-making; (4) scientific/technological solutions to environmental problems; (5) usefulness of school vs. media characterizations of science; (6) social constructivist vs. objectivist views of theories; (7) impact of cultural religious/ethical views on science; (8) tentativeness of scientific models, evidence and predictions; (9) civic control of technological developments. This analysis also revealed common relationships between student views which would not have been revealed under the original unique response model (URM) of VOSTS and also common viewpoint patterns that warrant further qualitative exploration.

Construction of a yeast artificial chromosome contig encompassing the chromosome 14 Alzheimer`s disease locus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sharma, V.; Bonnycastle, L.; Poorkai, P.

1994-09-01

We have constructed a yeast artificial chromosome (YAC) contig of chromosome 14q24.3 which encompasses the chromosome 14 Alzheimer`s disease locus (AD3). Determined by linkage analysis of early-onset Alzheimer`s disease kindreds, this interval is bounded by the genetic markers D14S61-D14S63 and spans approximately 15 centimorgans. The contig consists of 29 markers and 74 YACs of which 57 are defined by one or more sequence tagged sites (STSs). The STS markers comprise 5 genes, 16 short tandem repeat polymorphisms and 8 cDNA clones. An additional number of genes, expressed sequence tags and cDNA fragments have been identified and localized to the contigmore » by hybridization and sequence analysis of anonymous clones isolated by cDNA direct selection techniques. A minimal contig of about 15 YACs averaging 0.5-1.5 megabase in length will span this interval and is, at first approximation, in rough agreement with the genetic map. For two regions of the contig, our coverage has relied on L1/THE fingerprint and Alu-PCR hybridization data of YACs provided by CEPH/Genethon. We are currently developing sequence tagged sites from these to confirm the overlaps revealed by the fingerprint data. Among the genes which map to the contig are transforming growth factor beta 3, c-fos, and heat shock protein 2A (HSPA2). C-fos is not a candidate gene for AD3 based on the sequence analysis of affected and unaffected individuals. HSPA2 maps to the proximal edge of the contig and Calmodulin 1, a candidate gene from 4q24.3, maps outside of the region. The YAC contig is a framework physical map from which cosmid or P1 clone contigs can be constructed. As more genes and cDNAs are mapped, a highly resolved transcription map will emerge, a necessary step towards positionally cloning the AD3 gene.« less

Determination of Wolbachia Diversity in Butterflies from Western Ghats, India, by a Multigene Approach

PubMed Central

Salunke, Bipinchandra K.; Salunkhe, Rahul C.; Dhotre, Dhiraj P.; Walujkar, Sandeep A.; Khandagale, Avinash B.; Chaudhari, Rahul; Chandode, Rakesh K.; Ghate, Hemant V.; Patole, Milind S.; Werren, John H.

2012-01-01

Members of the genus Wolbachia are intracellular bacteria that are widespread in arthropods and establish diverse symbiotic associations with their hosts, ranging from mutualism to parasitism. Here we present the first detailed analyses of Wolbachia in butterflies from India with screening of 56 species. Twenty-nine species (52%) representing five families were positive for Wolbachia. This is the first report of Wolbachia infection in 27 of the 29 species; the other two were reported previously. This study also provides the first evidence of infection in the family Papilionidae. A striking diversity was observed among Wolbachia strains in butterfly hosts based on five multilocus sequence typing (MLST) genes, with 15 different sequence types (STs). Thirteen STs are new to the MLST database, whereas ST41 and ST125 were reported earlier. Some of the same host species from this study carried distinctly different Wolbachia strains, whereas the same or different butterfly hosts also harbored closely related Wolbachia strains. Butterfly-associated STs in the Indian sample originated by recombination and point mutation, further supporting the role of both processes in generating Wolbachia diversity. Recombination was detected only among the STs in this study and not in those from the MLST database. Most of the strains were remarkably similar in their wsp genotype, despite divergence in MLST. Only two wsp alleles were found among 25 individuals with complete hypervariable region (HVR) peptide profiles. Although both wsp and MLST show variability, MLST gives better separation between the strains. Completely different STs were characterized for the individuals sharing the same wsp alleles. PMID:22504801

Canine Soft Tissue Sarcomas: Can Being a Dog’s Best Friend Help a Child?

PubMed Central

Séguin, Bernard

2017-01-01

Soft tissue sarcomas (STSs) remain a therapeutic challenge for pediatric and adolescent and young adult (AYA) patients. Still today, surgery, radiation therapy, and chemotherapy remain the mainstay of treatment. Obstacles in developing new treatment approaches to improve the outcome are: few patients to enroll in clinical trials, and the diversity of tumor biology between histologic subtypes. Pet dogs may offer an additional strategy to discover and test new therapeutic avenues. The number of dogs diagnosed with a STS each year in the United States is estimated to be around 27,000 to 95,000. In comparison, approximately 900 children less than 20 years old and 1,500 AYAs between 15 and 29 years old are diagnosed with a STS each year in the United States. The mainstay for treatment of STSs in dogs is also surgery, with radiation therapy and chemotherapy when necessary. Similar to what is seen in humans, grade and stage are prognostic in dogs. In one comparative study of the histology and immunohistochemistry of canine STSs, most tumors were diagnosed as the human equivalent of undifferentiated sarcoma, spindle cell sarcoma, or unclassified spindle cell sarcoma. But much work remains to be done to fully assess the validity of canine STSs as a model. Gene expression analysis has been done in a limited number of canine STSs. Tissue banking, development of cell lines, and the ability to mobilize large-scale clinical trials will become essential in veterinary medicine to benefit both dogs and humans. PMID:29218302

Identification and mapping of two powdery mildew resistance genes in Triticum boeoticum L.

PubMed

Chhuneja, Parveen; Kumar, Krishan; Stirnweis, Daniel; Hurni, Severine; Keller, Beat; Dhaliwal, Harcharan S; Singh, Kuldeep

2012-04-01

Powdery mildew (PM) caused by Blumeria graminis f. sp. tritici (Bgt), is one of the important foliar diseases of wheat that can cause serious yield losses. Breeding for cultivars with diverse resources of resistance is the most promising approach for combating this disease. The diploid A genome progenitor species of wheat are an important resource for new variability for disease resistance genes. An accession of Triticum boeoticum (A(b)A(b)) showed resistance against a number of Bgt isolates, when tested using detached leaf segments. Inheritance studies in a recombinant inbred line population (RIL), developed from crosses of PM resistant T. boeoticum acc. pau5088 with a PM susceptible T. monococcum acc. pau14087, indicated the presence of two powdery mildew resistance genes in T. boeoticum acc. pau5088. Analysis of powdery mildew infection and molecular marker data of the RIL population revealed that both powdery mildew resistance genes are located on the long arm of chromosome 7A. Mapping was conducted using an integrated linkage map of 7A consisting of SSR, RFLP, STS, and DArT markers. These powdery mildew resistance genes are tentatively designated as PmTb7A.1 and PmTb7A.2. The PmTb7A.2 is closely linked to STS markers MAG2185 and MAG1759 derived from RFLP probes which are linked to powdery mildew resistance gene Pm1. This indicated that PmTb7A.2 might be allelic to Pm1. The PmTb7A.1, flanked by a DArT marker wPt4553 and an SSR marker Xcfa2019 in a 4.3 cM interval, maps proximal to PmT7A.2. PmTb7A.1 is putatively a new powdery mildew resistance gene. The powdery mildew resistance genes from T. boeoticum are currently being transferred to cultivated wheat background through marker-assisted backcrossing, using T. durum as bridging species.

Sulfation of minoxidil by multiple human cytosolic sulfotransferases.

PubMed

Anderson, R J; Kudlacek, P E; Clemens, D L

1998-02-20

Minoxidil is an antihypertensive agent and hair growth promoter that is metabolized by sulfation to the active compound, minoxidil sulfate. Thermostable phenol sulfotransferase (TS PST or P-PST) was initially thought to catalyze the reaction, and the enzyme was designated minoxidil sulfotransferase (MNX-ST). Information about human ST activities toward minoxidil would be useful in developing the capacity to predict individual responses to minoxidil based on tissue levels of STs. Therefore, human STs were studied from platelet homogenates, partially purified platelets, scalp skin high speed supernatants and COS-1 cell cDNA expressed preparations using a radiochemical enzymatic assay with minoxidil as the substrate. Studies showed the presence of TS PST, TL (thermolabile) PST and MNX-ST activities in human scalp skin. Biochemical properties and correlation studies suggested that in addition to TS PST, the TL PST activity, another ST activity or both were involved in the reaction. Partially purified human platelet TL PST tested with minoxidil and dopamine showed identical thermal stabilities and similar responses to the inhibitors 2,6-dichloro-4-nitrophenol (DCNP) and NaCl. To characterize the activity of TL PST toward minoxidil, several biochemical properties of the enzyme expressed from a human liver cDNA clone were investigated. When assayed with minoxidil and dopamine, thermal stabilities of the expressed enzyme were identical and IC50 values for the inhibitors DCNP and NaCl were similar. It was also demonstrated that cDNA encoded human liver dehydroepiandrosterone sulfotransferase and estrogen sulfotransferase contributed to the sulfation of minoxidil. The results confirm that at least four human STs contribute to minoxidil sulfation. MNX-ST activity represents a combination of ST activities. The data indicate that multiple ST activities should be taken into account in attempts to predict the regulation of minoxidil sulfation and individual responses to minoxidil.

Early detection of metallo-β-lactamase NDM-1- and OXA-23 carbapenemase-producing Acinetobacter baumannii in Libyan hospitals.

PubMed

Mathlouthi, Najla; El Salabi, Allaaeddin Ali; Ben Jomàa-Jemili, Mariem; Bakour, Sofiane; Al-Bayssari, Charbel; Zorgani, Abdulaziz A; Kraiema, Abdulmajeed; Elahmer, Omar; Okdah, Liliane; Rolain, Jean-Marc; Chouchani, Chedly

2016-07-01

Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The aim of this study was to characterise the molecular support of carbapenem-resistant A. baumannii clinical isolates recovered from two Libyan hospitals. Bacterial isolates were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Antibiotic susceptibility testing was performed using disk diffusion and Etest methods, and carbapenem resistance determinants were studied by PCR amplification and sequencing. Multilocus sequence typing (MLST) was performed for typing of the isolates. All 36 imipenem-resistant isolates tested were identified as A. baumannii. The blaOXA-23 gene was detected in 29 strains (80.6%). The metallo-β-lactamase blaNDM-1 gene was detected in eight isolates (22.2%), showing dissemination of multidrug-resistant (MDR) A. baumannii in Tripoli Medical Center and Burn and Plastic Surgery Hospital in Libya, including one isolate that co-expressed the blaOXA-23 gene. MLST revealed several sequence types (STs). Imipenem-resistant A. baumannii ST2 was the predominant clone (16/36; 44.4%). This study shows that NDM-1 and OXA-23 contribute to antibiotic resistance in Libyan hospitals and represents the first incidence of the association of these two carbapenemases in an autochthonous MDR A. baumannii isolated from patients in Libya, indicating that there is a longstanding infection control problem in these hospitals. Copyright © 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

DomeGene Experiment at Cell Biology Experiment Facility (CBEF) in JPM

NASA Image and Video Library

2009-03-18

ISS018-E-040985 (18 March 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 18 flight engineer, uses a computer at the Japanese Remote Manipulator System (JEM-RMS) work station in the Kibo laboratory of the International Space Station while Space Shuttle Discovery (STS-119) remains docked with the station.

DomeGene Experiment at Cell Biology Experiment Facility (CBEF) in JPM

NASA Image and Video Library

2009-03-18

ISS018-E-040986 (18 March 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 18 flight engineer, uses a computer at the Japanese Remote Manipulator System (JEM-RMS) work station in the Kibo laboratory of the International Space Station while Space Shuttle Discovery (STS-119) remains docked with the station.

Leptospira species molecular epidemiology in the genomic era.

PubMed

Caimi, K; Repetto, S A; Varni, V; Ruybal, P

2017-10-01

Leptospirosis is a zoonotic disease which global burden is increasing often related to climatic change. Hundreds of whole genome sequences from worldwide isolates of Leptospira spp. are available nowadays, together with online tools that permit to assign MLST sequence types (STs) directly from raw sequence data. In this work we have applied R7L-MLST to near 500 genomes and strains collection globally distributed. All 10 pathogenic species as well as intermediate were typed using this MLST scheme. The correlation observed between STs and serogroups in our previous work, is still satisfied with this higher dataset sustaining the implementation of MLST to assist serological classification as a complementary approach. Bayesian phylogenetic analysis of concatenated sequences from R7-MLST loci allowed us to resolve taxonomic inconsistencies but also showed that events such as recombination, gene conversion or lateral gene transfer played an important role in the evolution of Leptospira genus. Whole genome sequencing allows us to contribute with suitable epidemiologic information useful to apply in the design of control strategies and also in diagnostic methods for this illness. Copyright © 2017 Elsevier B.V. All rights reserved.

STS-114: Discovery Impromptu Briefing

NASA Technical Reports Server (NTRS)

2005-01-01

Dr. Griffin, NASA Administrator, is accompanied by members of The U.S. House of Representatives in this STS-114 Discovery Impromptu briefing. The U.S. House of Representatives present include: Sherwood Boehlert, House Science Committee Chairman, Senator Hutchinson, Sheila Jackson, 18th Congressional District Texas, Al Green, 9th Congressional District, Representative Jim Davis, Florida, and Gene Green, 29th District, Texas. Griffin talks about the problem that occurred with the external fuel tank sensor of the Space Shuttle Discovery and the effort NASA is pursuing to track the problem, and identify the root cause. He answers questions from the news media about the next steps for the Space Shuttle Discovery, time frame for the launch, and activities for the astronauts for the next few days.

KSC-2011-3308

NASA Image and Video Library

2011-04-29

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, Public Affairs Officer George Diller, Kennedy Director Bob Cabana, Space Shuttle Program Launch Integration Manager Mike Moses and Shuttle Launch Director Mike Leinbach participate in a news conference following the April 29 scrubbed launch attempt of space shuttle Endeavour. During the STS-134 countdown, fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) failed. Technicians later discovered that the Load Control Assembly-2 (LCA-2), which distributes power to nine shuttle systems, was the cause of the failure reading. The LCA-2 located in Endeavour's aft section will be replaced and systems will be retested before the launch is rescheduled. STS-134 will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. The mission also will be the final spaceflight for Endeavour. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-4204

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Employees admire space shuttle Endeavour as the landing convoy makes the vehicle safe and secure for towing to its processing hangar at NASA's Kennedy Space Center in Florida. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown on the Shuttle Landing Facility's Runway 15 was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4217

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- STS-134 Commander Mark Kelly addresses media gathered on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida following the successful landing of space shuttle Endeavour. From left, are the European Space Agency's Roberto Vittori, Pilot Greg H. Johnson, Kelly, and Mission Specialists Mike Fincke, Greg Chamitoff and Drew Feustel. The crew returned to Earth at 2:35 a.m. EDT on Runway 15, completing a 16-day, 6.5-million mile journey to the International Space Station. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4199

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Managers check out the heat shield tiles that protected space shuttle Endeavour on its successful trip home to the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida. Endeavour's final return from space completed the 16-day, 6.5-million-mile STS-134 mission. Main gear touchdown was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

KSC-2011-4216

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- STS-134 Commander Mark Kelly addresses media gathered on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida following the successful landing of space shuttle Endeavour. From left, are the European Space Agency's Roberto Vittori, Pilot Greg H. Johnson, Kelly, and Mission Specialists Mike Fincke, Greg Chamitoff and Drew Feustel. The crew returned to Earth at 2:35 a.m. EDT on Runway 15, completing a 16-day, 6.5-million mile journey to the International Space Station. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kim Shiflett

Neural correlates of audiovisual speech processing in a second language.

PubMed

Barrós-Loscertales, Alfonso; Ventura-Campos, Noelia; Visser, Maya; Alsius, Agnès; Pallier, Christophe; Avila Rivera, César; Soto-Faraco, Salvador

2013-09-01

Neuroimaging studies of audiovisual speech processing have exclusively addressed listeners' native language (L1). Yet, several behavioural studies now show that AV processing plays an important role in non-native (L2) speech perception. The current fMRI study measured brain activity during auditory, visual, audiovisual congruent and audiovisual incongruent utterances in L1 and L2. BOLD responses to congruent AV speech in the pSTS were stronger than in either unimodal condition in both L1 and L2. Yet no differences in AV processing were expressed according to the language background in this area. Instead, the regions in the bilateral occipital lobe had a stronger congruency effect on the BOLD response (congruent higher than incongruent) in L2 as compared to L1. According to these results, language background differences are predominantly expressed in these unimodal regions, whereas the pSTS is similarly involved in AV integration regardless of language dominance. Copyright © 2013 Elsevier Inc. All rights reserved.

Temperament and Early Stuttering Development: Cross-Sectional Findings From a Community Cohort.

PubMed

Kefalianos, Elaina; Onslow, Mark; Ukoumunne, Obioha C; Block, Susan; Reilly, Sheena

2017-04-14

The purpose of this study was to ascertain if there is an association between stuttering severity and behaviors and the expression of temperament characteristics, including precursors of anxiety. We studied temperament characteristics of a prospectively recruited community cohort of children who stutter (N = 173) at ages 3, 4, and 6 years using the Short Temperament Scale STS (Prior, Sanson, Smart & Oberklaid, 2000). Six of 131 statistical tests of association between stuttering severity and behaviors and temperament traits were statistically significant at the 5% level, which was no more than expected by chance alone. On the basis of parent responses to the STS, preschoolers who exhibited different levels of stuttering severity and behaviors did not generally express temperament traits differently from one another. Stuttering severity and stuttering behaviors were not associated with the precursors of anxiety. Overall, taking multiple tests into consideration, results show little evidence of association between stuttering severity and temperament up to 4 years of age or between stuttering behaviors and temperament up to 6 years of age.

Cloning a balanced t(9;11)(p24;q23.1) chromosomal translocation breakpoint segregating with bipolar affective disorder in a small pedigree

DOE Office of Scientific and Technical Information (OSTI.GOV)

Duggan, D.J.; Baysal, B.E.; Gollin, S.M.

A small multigenerational pedigree was previously identified in which a balanced 9;11 chromosomal translocation was cosegregating with bipolar affective disorder. We hypothesize that genes or gene regulatory sequences disrupted by the translocation are contributing to bipolar affective disorder in a dominant fashion. The general strategy involves (1) using somatic cell hybrids containing the derivative 9 or 11 chromosomes to identify the closest chromosome 9 and 11 flanking markers, (2) using the nearest markers as PCR and hybridization probes to isolate both normal DNA (YAC) and patient DNA (cosmid) adjacent to and incorporating the translocation breakpoint, and (3) identifying expressed sequencesmore » in the genomic DNA that may be disrupted by the translocation. From a fusion of the translocation patient cell line and a recipient hamster cell line, somatic cell hybrids were isolated which contain either the human derivative 9 or derivative 11 chromosome. Using PCR-based STS assays with these hybrids, the location of the translocation breakpoint was localized to an estimated 500 kb region at chromosome 11 band q23.1 and a 1 cM region in 9 band p24 (more telomeric than originally reported). From a large set of CEPH and Roswell Park yeast artificial chromosomes (YACs), six chromosome 11 YACs spanning the 11q23.1 breakpoint have now been identified. A combination of pulsed field gel eletrophoresis and YAC mapping has narrowed the chromosome 11 region to less than 430 kb. Current efforts are focused on generating new chromosome 11 probes within the flanking markers, mapping these probes back to the der(9) and der(11) containing hybrids and the chromosome 11 YAC mapping panel. As the region is physically narrowed, we will identify candidate genes whose expression may be altered by this t(9:11) translocation.« less

Effects and Responses to Spaceflight in the Mouse Retina

NASA Technical Reports Server (NTRS)

Zanello, Susana B.; Theriot, Corey; Westby, Christian; Boyle, Richard

2011-01-01

Several stress environmental factors are combined in a unique fashion during spaceflight, affecting living beings widely across their physiological systems. Recently, attention has been placed on vision changes in astronauts returning from long duration missions. Alterations include hyperoptic shift, globe flattening, choroidal folds and optic disc edema, which are probably associated with increased intracranial pressure. These observations justify a better characterization of the ocular health risks associated with spaceflight. This study investigates the impact of spaceflight on the biology of the mouse retina. Within a successful tissue sharing effort, eyes from albino Balb/cJ mice aboard STS-133 were collected for histological analysis and gene expression profiling of the retina at 1 and 7 days after landing. Both vivarium and AEM (Animal Enclosure Module) mice were used as ground controls. Oxidative stress-induced DNA damage was higher in the flight samples compared to controls on R+1, and decreased on R+7. A trend toward higher oxidative and cellular stress response gene expression was also observed on R+1 compared to AEM controls, and these levels decreased on R+7. Several genes coding for key antioxidant enzymes, namely, heme-oxygenase-1, peroxiredoxin, and catalase, were among those upregulated after flight. Likewise, NF B and TGFbeta1, were upregulated in one flight specimen that overall showed the most elevated oxidative stress markers on R+1. In addition, retinas from vivarium control mice evidenced higher oxidative stress markers, NF B and TGFbeta1, likely due to the more intense illumination in vivarium cages versus the AEM. These preliminary data suggest that spaceflight represents a source of environmental stress that translates into oxidative and cellular stress in the retina, which is partially reversible upon return to Earth. Further work is needed to dissect the contribution of the various spaceflight factors (microgravity, radiation) and to evaluate the impact of the stress response on retinal health.

Cytogenetic and molecular markers for detecting Aegilops uniaristata chromosomes in a wheat background.

PubMed

Gong, Wenping; Li, Guangrong; Zhou, Jianping; Li, Genying; Liu, Cheng; Huang, Chengyan; Zhao, Zhendong; Yang, Zujun

2014-09-01

Aegilops uniaristata has many agronomically useful traits that can be used for wheat breeding. So far, a Triticum turgidum - Ae. uniaristata amphiploid and one set of Chinese Spring (CS) - Ae. uniaristata addition lines have been produced. To guide Ae. uniaristata chromatin transformation from these lines into cultivated wheat through chromosome engineering, reliable cytogenetic and molecular markers specific for Ae. uniaristata chromosomes need to be developed. Standard C-banding shows that C-bands mainly exist in the centromeric regions of Ae. uniaristata but rarely at the distal ends. Fluorescence in situ hybridization (FISH) using (GAA)8 as a probe showed that the hybridization signal of chromosomes 1N-7N are different, thus (GAA)8 can be used to identify all Ae. uniaristata chromosomes in wheat background simultaneously. Moreover, a total of 42 molecular markers specific for Ae. uniaristata chromosomes were developed by screening expressed sequence tag - sequence tagged site (EST-STS), expressed sequence tag - simple sequence repeat (EST-SSR), and PCR-based landmark unique gene (PLUG) primers. The markers were subsequently localized using the CS - Ae. uniaristata addition lines and different wheat cultivars as controls. The cytogenetic and molecular markers developed herein will be helpful for screening and identifying wheat - Ae. uniaristata progeny.

GEM-TREND: a web tool for gene expression data mining toward relevant network discovery

PubMed Central

Feng, Chunlai; Araki, Michihiro; Kunimoto, Ryo; Tamon, Akiko; Makiguchi, Hiroki; Niijima, Satoshi; Tsujimoto, Gozoh; Okuno, Yasushi

2009-01-01

Background DNA microarray technology provides us with a first step toward the goal of uncovering gene functions on a genomic scale. In recent years, vast amounts of gene expression data have been collected, much of which are available in public databases, such as the Gene Expression Omnibus (GEO). To date, most researchers have been manually retrieving data from databases through web browsers using accession numbers (IDs) or keywords, but gene-expression patterns are not considered when retrieving such data. The Connectivity Map was recently introduced to compare gene expression data by introducing gene-expression signatures (represented by a set of genes with up- or down-regulated labels according to their biological states) and is available as a web tool for detecting similar gene-expression signatures from a limited data set (approximately 7,000 expression profiles representing 1,309 compounds). In order to support researchers to utilize the public gene expression data more effectively, we developed a web tool for finding similar gene expression data and generating its co-expression networks from a publicly available database. Results GEM-TREND, a web tool for searching gene expression data, allows users to search data from GEO using gene-expression signatures or gene expression ratio data as a query and retrieve gene expression data by comparing gene-expression pattern between the query and GEO gene expression data. The comparison methods are based on the nonparametric, rank-based pattern matching approach of Lamb et al. (Science 2006) with the additional calculation of statistical significance. The web tool was tested using gene expression ratio data randomly extracted from the GEO and with in-house microarray data, respectively. The results validated the ability of GEM-TREND to retrieve gene expression entries biologically related to a query from GEO. For further analysis, a network visualization interface is also provided, whereby genes and gene annotations are dynamically linked to external data repositories. Conclusion GEM-TREND was developed to retrieve gene expression data by comparing query gene-expression pattern with those of GEO gene expression data. It could be a very useful resource for finding similar gene expression profiles and constructing its gene co-expression networks from a publicly available database. GEM-TREND was designed to be user-friendly and is expected to support knowledge discovery. GEM-TREND is freely available at . PMID:19728865

GEM-TREND: a web tool for gene expression data mining toward relevant network discovery.

PubMed

Feng, Chunlai; Araki, Michihiro; Kunimoto, Ryo; Tamon, Akiko; Makiguchi, Hiroki; Niijima, Satoshi; Tsujimoto, Gozoh; Okuno, Yasushi

2009-09-03

DNA microarray technology provides us with a first step toward the goal of uncovering gene functions on a genomic scale. In recent years, vast amounts of gene expression data have been collected, much of which are available in public databases, such as the Gene Expression Omnibus (GEO). To date, most researchers have been manually retrieving data from databases through web browsers using accession numbers (IDs) or keywords, but gene-expression patterns are not considered when retrieving such data. The Connectivity Map was recently introduced to compare gene expression data by introducing gene-expression signatures (represented by a set of genes with up- or down-regulated labels according to their biological states) and is available as a web tool for detecting similar gene-expression signatures from a limited data set (approximately 7,000 expression profiles representing 1,309 compounds). In order to support researchers to utilize the public gene expression data more effectively, we developed a web tool for finding similar gene expression data and generating its co-expression networks from a publicly available database. GEM-TREND, a web tool for searching gene expression data, allows users to search data from GEO using gene-expression signatures or gene expression ratio data as a query and retrieve gene expression data by comparing gene-expression pattern between the query and GEO gene expression data. The comparison methods are based on the nonparametric, rank-based pattern matching approach of Lamb et al. (Science 2006) with the additional calculation of statistical significance. The web tool was tested using gene expression ratio data randomly extracted from the GEO and with in-house microarray data, respectively. The results validated the ability of GEM-TREND to retrieve gene expression entries biologically related to a query from GEO. For further analysis, a network visualization interface is also provided, whereby genes and gene annotations are dynamically linked to external data repositories. GEM-TREND was developed to retrieve gene expression data by comparing query gene-expression pattern with those of GEO gene expression data. It could be a very useful resource for finding similar gene expression profiles and constructing its gene co-expression networks from a publicly available database. GEM-TREND was designed to be user-friendly and is expected to support knowledge discovery. GEM-TREND is freely available at http://cgs.pharm.kyoto-u.ac.jp/services/network.

Reconstructing dynamic mental models of facial expressions in prosopagnosia reveals distinct representations for identity and expression.

PubMed

Richoz, Anne-Raphaëlle; Jack, Rachael E; Garrod, Oliver G B; Schyns, Philippe G; Caldara, Roberto

2015-04-01

The human face transmits a wealth of signals that readily provide crucial information for social interactions, such as facial identity and emotional expression. Yet, a fundamental question remains unresolved: does the face information for identity and emotional expression categorization tap into common or distinct representational systems? To address this question we tested PS, a pure case of acquired prosopagnosia with bilateral occipitotemporal lesions anatomically sparing the regions that are assumed to contribute to facial expression (de)coding (i.e., the amygdala, the insula and the posterior superior temporal sulcus--pSTS). We previously demonstrated that PS does not use information from the eye region to identify faces, but relies on the suboptimal mouth region. PS's abnormal information use for identity, coupled with her neural dissociation, provides a unique opportunity to probe the existence of a dichotomy in the face representational system. To reconstruct the mental models of the six basic facial expressions of emotion in PS and age-matched healthy observers, we used a novel reverse correlation technique tracking information use on dynamic faces. PS was comparable to controls, using all facial features to (de)code facial expressions with the exception of fear. PS's normal (de)coding of dynamic facial expressions suggests that the face system relies either on distinct representational systems for identity and expression, or dissociable cortical pathways to access them. Interestingly, PS showed a selective impairment for categorizing many static facial expressions, which could be accounted for by her lesion in the right inferior occipital gyrus. PS's advantage for dynamic facial expressions might instead relate to a functionally distinct and sufficient cortical pathway directly connecting the early visual cortex to the spared pSTS. Altogether, our data provide critical insights on the healthy and impaired face systems, question evidence of deficits obtained from patients by using static images of facial expressions, and offer novel routes for patient rehabilitation. Copyright © 2014 Elsevier Ltd. All rights reserved.

KSC-2011-3572

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour's six STS-134 astronauts, dressed in their orange launch-and-entry suits, wave to news media and other spectators as they walk toward the Astrovan parked in front of the Operations and Checkout Building at NASA's Kennedy Space Center in Florida. The modified Airstream recreational vehicle, which has transported astronauts to their spacecraft since 1984, will take the crew to Launch Pad 39A. Leading the way are Pilot Greg H. Johnson, front left, and Commander Mark Kelly, front right. Behind Johnson are Mission Specialists Roberto Vittori with the European Space Agency and Greg Chamitoff. Behind Kelly are Mission Specialists Michael Fincke and Andrew Feustel. STS-134 will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. May 16 at 8:56 a.m. will be the second launch attempt for Endeavour. The first attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

Close Genetic Relationship between Legionella pneumophila Serogroup 1 Isolates from Sputum Specimens and Puddles on Roads, as Determined by Sequence-Based Typing

PubMed Central

Kanatani, Jun-ichi; Isobe, Junko; Kimata, Keiko; Shima, Tomoko; Shimizu, Miwako; Kura, Fumiaki; Sata, Tetsutaro

2013-01-01

We investigated the prevalence of Legionella species isolated from puddles on asphalt roads. In addition, we carried out sequence-based typing (SBT) analysis on the genetic relationship between L. pneumophila serogroup 1 (SG 1) isolates from puddles and from stock strains previously obtained from sputum specimens and public baths. Sixty-nine water samples were collected from puddles on roads at 6 fixed locations. Legionella species were detected in 33 samples (47.8%) regardless of season. Among the 325 isolates from puddles, strains of L. pneumophila SG 1, a major causative agent of Legionnaires' disease, were the most frequently isolated (n = 62, 19.1%). Sixty-two isolates of L. pneumophila SG 1 from puddles were classified into 36 sequence types (STs) by SBT. ST120 and ST48 were identified as major STs. Environmental ST120 strains from puddles were found for the first time in this study. Among the 14 STs of the clinical isolates (n = 19), 4 STs (n = 6, 31.6%), including ST120, were also detected in isolates from puddles on roads, and the sources of infection in these cases remained unclear. The lag-1 gene, a tentative marker for clinical isolates, was prevalent in puddle isolates (61.3%). Our findings suggest that puddles on asphalt roads serve as potential reservoirs for L. pneumophila in the environment. PMID:23603681

Close genetic relationship between Legionella pneumophila serogroup 1 isolates from sputum specimens and puddles on roads, as determined by sequence-based typing.

PubMed

Kanatani, Jun-ichi; Isobe, Junko; Kimata, Keiko; Shima, Tomoko; Shimizu, Miwako; Kura, Fumiaki; Sata, Tetsutaro; Watahiki, Masanori

2013-07-01

We investigated the prevalence of Legionella species isolated from puddles on asphalt roads. In addition, we carried out sequence-based typing (SBT) analysis on the genetic relationship between L. pneumophila serogroup 1 (SG 1) isolates from puddles and from stock strains previously obtained from sputum specimens and public baths. Sixty-nine water samples were collected from puddles on roads at 6 fixed locations. Legionella species were detected in 33 samples (47.8%) regardless of season. Among the 325 isolates from puddles, strains of L. pneumophila SG 1, a major causative agent of Legionnaires' disease, were the most frequently isolated (n = 62, 19.1%). Sixty-two isolates of L. pneumophila SG 1 from puddles were classified into 36 sequence types (STs) by SBT. ST120 and ST48 were identified as major STs. Environmental ST120 strains from puddles were found for the first time in this study. Among the 14 STs of the clinical isolates (n = 19), 4 STs (n = 6, 31.6%), including ST120, were also detected in isolates from puddles on roads, and the sources of infection in these cases remained unclear. The lag-1 gene, a tentative marker for clinical isolates, was prevalent in puddle isolates (61.3%). Our findings suggest that puddles on asphalt roads serve as potential reservoirs for L. pneumophila in the environment.

Neighboring Genes Show Correlated Evolution in Gene Expression

PubMed Central

Ghanbarian, Avazeh T.; Hurst, Laurence D.

2015-01-01

When considering the evolution of a gene’s expression profile, we commonly assume that this is unaffected by its genomic neighborhood. This is, however, in contrast to what we know about the lack of autonomy between neighboring genes in gene expression profiles in extant taxa. Indeed, in all eukaryotic genomes genes of similar expression-profile tend to cluster, reflecting chromatin level dynamics. Does it follow that if a gene increases expression in a particular lineage then the genomic neighbors will also increase in their expression or is gene expression evolution autonomous? To address this here we consider evolution of human gene expression since the human-chimp common ancestor, allowing for both variation in estimation of current expression level and error in Bayesian estimation of the ancestral state. We find that in all tissues and both sexes, the change in gene expression of a focal gene on average predicts the change in gene expression of neighbors. The effect is highly pronounced in the immediate vicinity (<100 kb) but extends much further. Sex-specific expression change is also genomically clustered. As genes increasing their expression in humans tend to avoid nuclear lamina domains and be enriched for the gene activator 5-hydroxymethylcytosine, we conclude that, most probably owing to chromatin level control of gene expression, a change in gene expression of one gene likely affects the expression evolution of neighbors, what we term expression piggybacking, an analog of hitchhiking. PMID:25743543

Correlation between Ureaplasma subgroup 2 and genitourinary tract disease outcomes revealed by an expanded multilocus sequence typing (eMLST) scheme.

PubMed

Zhang, Jun; Kong, Yingying; Ruan, Zhi; Huang, Jun; Song, Tiejun; Song, Jingjuan; Jiang, Yan; Yu, Yunsong; Xie, Xinyou

2014-01-01

The multilocus sequence typing (MLST) scheme of Ureaplasma based on four housekeeping genes (ftsH, rpL22, valS, and thrS) was described in our previous study; here we introduced an expanded MLST (eMLST) scheme with improved discriminatory power, which was developed by adding two putative virulence genes (ureG and mba-np1) to the original MLST scheme. To evaluate the discriminatory power of eMLST, a total of 14 reference strains of Ureaplasma serovars and 269 clinical strains (134 isolated from symptomatic patients and 135 obtained from asymptomatic persons) were investigated. Our study confirmed that all 14 serotype strains could successfully be differentiated into 14 eMLST STs (eSTs), while some of them could not even be differentiated by the MLST, and a total of 136 eSTs were identified among the clinical isolates we investigated. In addition, phylogenetic analysis indicated that two genetically significantly distant clusters (cluster I and II) were revealed and most clinical isolates were located in cluster I. These findings were in accordance with and further support for the concept of two well-known genetic lineages (Ureaplasma parvum and Ureaplasma urealyticum) in our previous study. Interestingly, although both clusters were associated with clinical manifestation, the sub-group 2 of cluster II had pronounced and adverse effect on patients and might be a potential risk factor for clinical outcomes. In conclusion, the eMLST scheme offers investigators a highly discriminative typing tool that is capable for precise epidemiological investigations and clinical relevance of Ureaplasma.

Correlation between Ureaplasma Subgroup 2 and Genitourinary Tract Disease Outcomes Revealed by an Expanded Multilocus Sequence Typing (eMLST) Scheme

PubMed Central

Ruan, Zhi; Huang, Jun; Song, Tiejun; Song, Jingjuan; Jiang, Yan; Yu, Yunsong; Xie, Xinyou

2014-01-01

The multilocus sequence typing (MLST) scheme of Ureaplasma based on four housekeeping genes (ftsH, rpL22, valS, and thrS) was described in our previous study; here we introduced an expanded MLST (eMLST) scheme with improved discriminatory power, which was developed by adding two putative virulence genes (ureG and mba-np1) to the original MLST scheme. To evaluate the discriminatory power of eMLST, a total of 14 reference strains of Ureaplasma serovars and 269 clinical strains (134 isolated from symptomatic patients and 135 obtained from asymptomatic persons) were investigated. Our study confirmed that all 14 serotype strains could successfully be differentiated into 14 eMLST STs (eSTs), while some of them could not even be differentiated by the MLST, and a total of 136 eSTs were identified among the clinical isolates we investigated. In addition, phylogenetic analysis indicated that two genetically significantly distant clusters (cluster I and II) were revealed and most clinical isolates were located in cluster I. These findings were in accordance with and further support for the concept of two well-known genetic lineages (Ureaplasma parvum and Ureaplasma urealyticum) in our previous study. Interestingly, although both clusters were associated with clinical manifestation, the sub-group 2 of cluster II had pronounced and adverse effect on patients and might be a potential risk factor for clinical outcomes. In conclusion, the eMLST scheme offers investigators a highly discriminative typing tool that is capable for precise epidemiological investigations and clinical relevance of Ureaplasma. PMID:25093900

Association of virulence plasmid and antibiotic resistance determinants with chromosomal multilocus genotypes in Mexican Salmonella enterica serovar Typhimurium strains

PubMed Central

2009-01-01

Background Bacterial genomes are mosaic structures composed of genes present in every strain of the same species (core genome), and genes present in some but not all strains of a species (accessory genome). The aim of this study was to compare the genetic diversity of core and accessory genes of a Salmonella enterica subspecies enterica serovar Typhimurium (Typhimurium) population isolated from food-animal and human sources in four regions of Mexico. Multilocus sequence typing (MLST) and macrorestriction fingerprints by pulsed-field gel electrophoresis (PFGE) were used to address the core genetic variation, and genes involved in pathogenesis and antibiotic resistance were selected to evaluate the accessory genome. Results We found a low genetic diversity for both housekeeping and accessory genes. Sequence type 19 (ST19) was supported as the founder genotype of STs 213, 302 and 429. We found a temporal pattern in which the derived ST213 is replacing the founder ST19 in the four geographic regions analyzed and a geographic trend in the number of resistance determinants. The distribution of the accessory genes was not random among chromosomal genotypes. We detected strong associations among the different accessory genes and the multilocus chromosomal genotypes (STs). First, the Salmonella virulence plasmid (pSTV) was found mostly in ST19 isolates. Second, the plasmid-borne betalactamase cmy-2 was found only in ST213 isolates. Third, the most abundant integron, IP-1 (dfrA12, orfF and aadA2), was found only in ST213 isolates. Fourth, the Salmonella genomic island (SGI1) was found mainly in a subgroup of ST19 isolates carrying pSTV. The mapping of accessory genes and multilocus genotypes on the dendrogram derived from macrorestiction fingerprints allowed the establishment of genetic subgroups within the population. Conclusion Despite the low levels of genetic diversity of core and accessory genes, the non-random distribution of the accessory genes across chromosomal backgrounds allowed us to discover genetic subgroups within the population. This study provides information about the importance of the accessory genome in generating genetic variability within a bacterial population. PMID:19573249

KSC-2011-1959

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is suspended vertically over the transfer aisle. The spacecraft will be moved into a high bay where it will be installed to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1953

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is suspended above the transfer aisle. The spacecraft will be lifted into a vertical position and moved into a high bay where it will be attached to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1955

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, an overhead crane slowly lifts shuttle Endeavour into a vertical position. The spacecraft will be moved into a high bay where it will be attached to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1950

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers disconnect shuttle Endeavour from its transport. An attached overhead crane will lift the spacecraft into a high bay where it will be attached to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1948

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers attach an overhead crane to shuttle Endeavour. The crane will lift the spacecraft into a high bay where it will be attached to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1973

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is being lifted and moved into a high bay where it will be attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-1958

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is suspended vertically over the transfer aisle. The spacecraft will be moved into a high bay where it will be installed to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1949

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers attach an overhead crane to shuttle Endeavour. The crane will lift the spacecraft into a high bay where it will be attached to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1974

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is being lifted and moved into a high bay where it will be attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-1954

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is suspended above the transfer aisle. The spacecraft will be lifted into a vertical position and moved into a high bay where it will be attached to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1947

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers attach an overhead crane to shuttle Endeavour. The crane will lift the spacecraft into a high bay where it will be attached to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-1957

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is suspended vertically over the transfer aisle. The spacecraft will be moved into a high bay where it will be installed to the waiting external fuel tank and solid rocket boosters. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

Identification of Human HK Genes and Gene Expression Regulation Study in Cancer from Transcriptomics Data Analysis

PubMed Central

Zhang, Zhang; Liu, Jingxing; Wu, Jiayan; Yu, Jun

2013-01-01

The regulation of gene expression is essential for eukaryotes, as it drives the processes of cellular differentiation and morphogenesis, leading to the creation of different cell types in multicellular organisms. RNA-Sequencing (RNA-Seq) provides researchers with a powerful toolbox for characterization and quantification of transcriptome. Many different human tissue/cell transcriptome datasets coming from RNA-Seq technology are available on public data resource. The fundamental issue here is how to develop an effective analysis method to estimate expression pattern similarities between different tumor tissues and their corresponding normal tissues. We define the gene expression pattern from three directions: 1) expression breadth, which reflects gene expression on/off status, and mainly concerns ubiquitously expressed genes; 2) low/high or constant/variable expression genes, based on gene expression level and variation; and 3) the regulation of gene expression at the gene structure level. The cluster analysis indicates that gene expression pattern is higher related to physiological condition rather than tissue spatial distance. Two sets of human housekeeping (HK) genes are defined according to cell/tissue types, respectively. To characterize the gene expression pattern in gene expression level and variation, we firstly apply improved K-means algorithm and a gene expression variance model. We find that cancer-associated HK genes (a HK gene is specific in cancer group, while not in normal group) are expressed higher and more variable in cancer condition than in normal condition. Cancer-associated HK genes prefer to AT-rich genes, and they are enriched in cell cycle regulation related functions and constitute some cancer signatures. The expression of large genes is also avoided in cancer group. These studies will help us understand which cell type-specific patterns of gene expression differ among different cell types, and particularly for cancer. PMID:23382867

Neighboring Genes Show Correlated Evolution in Gene Expression.

PubMed

Ghanbarian, Avazeh T; Hurst, Laurence D

2015-07-01

When considering the evolution of a gene's expression profile, we commonly assume that this is unaffected by its genomic neighborhood. This is, however, in contrast to what we know about the lack of autonomy between neighboring genes in gene expression profiles in extant taxa. Indeed, in all eukaryotic genomes genes of similar expression-profile tend to cluster, reflecting chromatin level dynamics. Does it follow that if a gene increases expression in a particular lineage then the genomic neighbors will also increase in their expression or is gene expression evolution autonomous? To address this here we consider evolution of human gene expression since the human-chimp common ancestor, allowing for both variation in estimation of current expression level and error in Bayesian estimation of the ancestral state. We find that in all tissues and both sexes, the change in gene expression of a focal gene on average predicts the change in gene expression of neighbors. The effect is highly pronounced in the immediate vicinity (<100 kb) but extends much further. Sex-specific expression change is also genomically clustered. As genes increasing their expression in humans tend to avoid nuclear lamina domains and be enriched for the gene activator 5-hydroxymethylcytosine, we conclude that, most probably owing to chromatin level control of gene expression, a change in gene expression of one gene likely affects the expression evolution of neighbors, what we term expression piggybacking, an analog of hitchhiking. © The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

Identification and mapping of Sr46 from Aegilops tauschii accession CIae 25 conferring resistance to race TTKSK (Ug99) of wheat stem rust pathogen.

PubMed

Yu, Guotai; Zhang, Qijun; Friesen, Timothy L; Rouse, Matthew N; Jin, Yue; Zhong, Shaobin; Rasmussen, Jack B; Lagudah, Evans S; Xu, Steven S

2015-03-01

Mapping studies confirm that resistance to Ug99 race of stem rust pathogen in Aegilops tauschii accession Clae 25 is conditioned by Sr46 and markers linked to the gene were developed for marker-assisted selection. The race TTKSK (Ug99) of Puccinia graminis f. sp. tritici, the causal pathogen for wheat stem rust, is considered as a major threat to global wheat production. To address this threat, researchers across the world have been devoted to identifying TTKSK-resistant genes. Here, we report the identification and mapping of a stem rust resistance gene in Aegilops tauschii accession CIae 25 that confers resistance to TTKSK and the development of molecular markers for the gene. An F2 population of 710 plants from an Ae. tauschii cross CIae 25 × AL8/78 were first evaluated against race TPMKC. A set of 14 resistant and 116 susceptible F2:3 families from the F2 plants were then evaluated for their reactions to TTKSK. Based on the tests, 179 homozygous susceptible F2 plants were selected as the mapping population to identify the simple sequence repeat (SSR) and sequence tagged site (STS) markers linked to the gene by bulk segregant analysis. A dominant stem rust resistance gene was identified and mapped with 16 SSR and five new STS markers to the deletion bin 2DS5-0.47-1.00 of chromosome arm 2DS in which Sr46 was located. Molecular marker and stem rust tests on CIae 25 and two Ae. tauschii accessions carrying Sr46 confirmed that the gene in CIae 25 is Sr46. This study also demonstrated that Sr46 is temperature-sensitive being less effective at low temperatures. The marker validation indicated that two closely linked markers Xgwm210 and Xwmc111 can be used for marker-assisted selection of Sr46 in wheat breeding programs.

Molecular characterization of resistance, virulence and clonality in vancomycin-resistant Enterococcus faecium and Enterococcus faecalis: A hospital-based study in Beijing, China.

PubMed

Yang, Jing-xian; Li, Tong; Ning, Yong-zhong; Shao, Dong-hua; Liu, Jing; Wang, Shu-qin; Liang, Guo-wei

2015-07-01

The incidence of vancomycin-resistant enterococcus (VRE) in China is increasing, the molecular epidemiology of VRE in China is only partly known. This study was conducted to assess the molecular characterization of resistance, virulence and clonality of 69 vancomycin-resistant Enterococcus faecium (VREfm) and seven vancomycin-resistant Enterococcus faecalis (VREfs) isolates obtained from a Chinese hospital between July 2011 and July 2013. The glycopeptide resistance genes (VanA and VanB) were screened by multiplex PCR. The presence of five putative virulence genes (esp, gelE, asa1, hyl and cylA) were evaluated by another multiplex PCR. Multilocus sequence typing (MLST) scheme was used to assess the clonality. All 76 VRE isolates exhibited VanA phenotype and harbored VanA gene. Esp was the only gene detected both in VREfm and VREfs strains, accounting for 89.9% and 42.9%, respectively. The hyl gene was merely positive in 27.5% of VREfm strains. MLST analysis demonstrated three STs (ST6, ST4 and ST470) in VREfs and twelve STs (ST78, ST571, ST17, ST564, ST389, ST18, ST547, ST341, ST414, ST343, ST262 and ST203) in VREfm, which were all designated as CC17 by eBURST algorithm. An outbreak of VREfm belonging to ST571 was found to happen within the neurology ward in this hospital. To our knowledge, this is the first report of ST6 (CC2) VREfs strains in China and the first outbreak report of VREfm strains belonging to ST571 around the world. Our data could offer important information for understanding the molecular features of VRE in China. Copyright © 2015 Elsevier B.V. All rights reserved.

Molecular epidemiology of Pseudomonas aeruginosa clinical isolates from Korea producing β-lactamases with extended-spectrum activity.

PubMed

Bae, Il Kwon; Suh, Borum; Jeong, Seok Hoon; Wang, Kang-Kyun; Kim, Yong-Rok; Yong, Dongeun; Lee, Kyungwon

2014-07-01

This study was performed to investigate the prevalence and molecular epidemiology of Pseudomonas aeruginosa isolates from Korea that produce enzymes with extended-spectrum (ES) activity to β-lactams. A total of 205 non-duplicate P. aeruginosa clinical isolates were collected from 18 university hospitals in Korea. PCR and sequencing experiments were performed to identify genes encoding β-lactamases. PCR mapping and sequencing of the regions surrounding the β-lactamase genes were performed. Multilocus sequence typing experiments were performed. The most common sequence type (ST) was ST235 (n = 96), and 2 single-locus variants of ST235, ST1015 (n = 1) and ST1162 (n = 1), were also identified. These 3 STs were grouped as a clonal complex (CC), CC235. The remaining 107 isolates were identified as 59 different STs. Isolates belonging to CC235 showed higher rates of non-susceptibility to imipenem (85.4% versus 47.7%) and meropenem (92.7% versus 52.3%) compared to non-CC235 isolates. All the metallo-β-lactamase (MBL)-producing isolates were identified as CC235, except for 1 ST591. Genes encoding OXA-17 and OXA-142 were detected in 1 isolate and 4 isolates of CC235, respectively; while the bla(SHV-12) gene was detected in 4 non-CC235 isolates. Class A and D β-lactamases with ES activity play a role in acquiring ceftazidime resistance in P. aeruginosa in Korea. Production of IMP-6 and VIM-2 MBLs is the main mechanisms in acquiring resistance to ceftazidime and carbapenems in P. aeruginosa isolates in Korea. Clonal spread of P. aeruginosa CC235 may be an important conduit for the dissemination of MBL genes in Korea. Copyright © 2014 Elsevier Inc. All rights reserved.

Application of community phylogenetic approaches to understand gene expression: differential exploration of venom gene space in predatory marine gastropods.

PubMed

Chang, Dan; Duda, Thomas F

2014-06-05

Predatory marine gastropods of the genus Conus exhibit substantial variation in venom composition both within and among species. Apart from mechanisms associated with extensive turnover of gene families and rapid evolution of genes that encode venom components ('conotoxins'), the evolution of distinct conotoxin expression patterns is an additional source of variation that may drive interspecific differences in the utilization of species' 'venom gene space'. To determine the evolution of expression patterns of venom genes of Conus species, we evaluated the expression of A-superfamily conotoxin genes of a set of closely related Conus species by comparing recovered transcripts of A-superfamily genes that were previously identified from the genomes of these species. We modified community phylogenetics approaches to incorporate phylogenetic history and disparity of genes and their expression profiles to determine patterns of venom gene space utilization. Less than half of the A-superfamily gene repertoire of these species is expressed, and only a few orthologous genes are coexpressed among species. Species exhibit substantially distinct expression strategies, with some expressing sets of closely related loci ('under-dispersed' expression of available genes) while others express sets of more disparate genes ('over-dispersed' expression). In addition, expressed genes show higher dN/dS values than either unexpressed or ancestral genes; this implies that expression exposes genes to selection and facilitates rapid evolution of these genes. Few recent lineage-specific gene duplicates are expressed simultaneously, suggesting that expression divergence among redundant gene copies may be established shortly after gene duplication. Our study demonstrates that venom gene space is explored differentially by Conus species, a process that effectively permits the independent and rapid evolution of venoms in these species.

Serotype distribution, antimicrobial resistance, and molecular characterization of invasive group B Streptococcus isolates recovered from Chinese neonates.

PubMed

Wang, Ping; Ma, Zhuoya; Tong, Jingjing; Zhao, Ruizhen; Shi, Wei; Yu, Sangjie; Yao, Kaihu; Zheng, Yuejie; Yang, Yonghong

2015-08-01

Group B Streptococcus (GBS) is an important neonatal pathogen associated with high morbidity and mortality in developed countries. However, data describing neonatal GBS disease in developing countries, particularly in Asia, are largely incomplete. The aim of this study was to determine the serotype distribution, antimicrobial resistance, and molecular characteristics of invasive GBS isolates recovered from Chinese neonates. From 2008 to 2013, 40 GBS isolates were recovered from infected neonates less than 3 months of age. All isolates were identified with the CAMP test and commercially available techniques. Serotyping was performed by latex agglutination. Antibiotic susceptibility was tested with Etest strips and the disk diffusion method. Multilocus sequence typing and erythromycin resistance gene detection (ermB and mefA) were performed by PCR. Four serotypes were identified. Serotype III (85%) was the most prevalent, followed by Ia (7.5%), Ib (5%), and V (2.5%). All isolates were sensitive to penicillin, ceftriaxone, and levofloxacin. However, resistance to erythromycin (92.5%), clindamycin (87.5%), and tetracycline (100%) was observed. Among erythromycin-resistant isolates, 73.0% carried the ermB gene alone, 5.4% carried the mefA gene alone, and 21.6% expressed both ermB and mefA genes. A total of seven sequence types (STs) were identified; the most prevalent was ST17, accounting for 80% of all isolates. Further, serotype III isolates contained ST17 (94.2%), ST19 (2.9%), and ST650 (2.9%). Serotype distribution, antimicrobial susceptibility, and sequence type characterization in Asia and in other global regions may contribute to improve the prevention and treatment of neonatal GBS infections. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

The neuroendocrine phenotype, genomic profile and therapeutic sensitivity of GEPNET cell lines

PubMed Central

Hofving, Tobias; Arvidsson, Yvonne; Almobarak, Bilal; Inge, Linda; Pfragner, Roswitha; Persson, Marta; Stenman, Göran; Kristiansson, Erik; Johanson, Viktor; Nilsson, Ola

2018-01-01

Experimental models of neuroendocrine tumour disease are scarce, and no comprehensive characterisation of existing gastroenteropancreatic neuroendocrine tumour (GEPNET) cell lines has been reported. In this study, we aimed to define the molecular characteristics and therapeutic sensitivity of these cell lines. We therefore performed immunophenotyping, copy number profiling, whole-exome sequencing and a large-scale inhibitor screening of seven GEPNET cell lines. Four cell lines, GOT1, P-STS, BON-1 and QGP-1, displayed a neuroendocrine phenotype while three others, KRJ-I, L-STS and H-STS, did not. Instead, these three cell lines were identified as lymphoblastoid. Characterisation of remaining authentic GEPNET cell lines by copy number profiling showed that GOT1, among other chromosomal alterations, harboured losses on chromosome 18 encompassing the SMAD4 gene, while P-STS had a loss on 11q. BON-1 had a homozygous loss of CDKN2A and CDKN2B, and QGP-1 harboured amplifications of MDM2 and HMGA2. Whole-exome sequencing revealed both disease-characteristic mutations (e.g. ATRX mutation in QGP-1) and, for patient tumours, rare genetic events (e.g. TP53 mutation in P-STS, BON-1 and QGP-1). A large-scale inhibitor screening showed that cell lines from pancreatic NETs to a greater extent, when compared to small intestinal NETs, were sensitive to inhibitors of MEK. Similarly, neuroendocrine NET cells originating from the small intestine were considerably more sensitive to a group of HDAC inhibitors. Taken together, our results provide a comprehensive characterisation of GEPNET cell lines, demonstrate their relevance as neuroendocrine tumour models and explore their therapeutic sensitivity to a broad range of inhibitors. PMID:29444910

Multilocus Sequence Typing Analysis of Staphylococcus lugdunensis Implies a Clonal Population Structure

PubMed Central

Chassain, Benoît; Lemée, Ludovic; Didi, Jennifer; Thiberge, Jean-Michel; Brisse, Sylvain; Pons, Jean-Louis

2012-01-01

Staphylococcus lugdunensis is recognized as one of the major pathogenic species within the genus Staphylococcus, even though it belongs to the coagulase-negative group. A multilocus sequence typing (MLST) scheme was developed to study the genetic relationships and population structure of 87 S. lugdunensis isolates from various clinical and geographic sources by DNA sequence analysis of seven housekeeping genes (aroE, dat, ddl, gmk, ldh, recA, and yqiL). The number of alleles ranged from four (gmk and ldh) to nine (yqiL). Allelic profiles allowed the definition of 20 different sequence types (STs) and five clonal complexes. The 20 STs lacked correlation with geographic source. Isolates recovered from hematogenic infections (blood or osteoarticular isolates) or from skin and soft tissue infections did not cluster in separate lineages. Penicillin-resistant isolates clustered mainly in one clonal complex, unlike glycopeptide-tolerant isolates, which did not constitute a distinct subpopulation within S. lugdunensis. Phylogenies from the sequences of the seven individual housekeeping genes were congruent, indicating a predominantly mutational evolution of these genes. Quantitative analysis of the linkages between alleles from the seven loci revealed a significant linkage disequilibrium, thus confirming a clonal population structure for S. lugdunensis. This first MLST scheme for S. lugdunensis provides a new tool for investigating the macroepidemiology and phylogeny of this unusually virulent coagulase-negative Staphylococcus. PMID:22785196

An evaluation of traffic-awareness campaign videos: empathy induction is associated with brain function within superior temporal sulcus.

PubMed

Zelinková, Jana; Shaw, Daniel J; Mareček, Radek; Mikl, Michal; Urbánek, Tomáš; Havlíčková, Darina; Zámečník, Petr; Haitová, Petra; Brázdil, Milan

2014-08-12

Acting appropriately within social contexts requires an ability to appreciate others' mental and emotional states. Indeed, some campaign programs designed to reduce anti-social behaviour seek to elicit empathy for the victims. The effectiveness of these campaigns can be evaluated according to the degree to which they induce such responses, but by applying neuroscientific techniques this can be done at the behavioural and neurophysiological level. Neuroimaging studies aimed at identifying the neural mechanisms behind such socio-cognitive and -emotional processes frequently reveal the role of the superior temporal sulcus (STS). We applied this knowledge to assess the effectiveness of traffic-awareness campaign adverts to induce empathic expression. Functional magnetic resonance imaging (fMRI) data were acquired from 20 healthy male volunteers as they watched these campaign videos consisting of a dramatic sequence of events and catastrophic endings, and control videos without such dramatic endings. Among other structures, a significantly greater neural response was observed within bilateral STS, particularly within the right hemisphere, during the observation of campaign relative to control videos. Furthermore, activation in these brain regions correlated with the subjects' empathic expression. Our results develop our understanding of the role of STS in social cognition. Moreover, our data demonstrate the utility of neuroscientific methods when evaluating the effectiveness of campaign videos in terms of their ability to elicit empathic responses. Our study also demonstrates the utility of these specific stimuli for future neuroscientific research.

An evaluation of traffic-awareness campaign videos: empathy induction is associated with brain function within superior temporal sulcus

PubMed Central

2014-01-01

Acting appropriately within social contexts requires an ability to appreciate others’ mental and emotional states. Indeed, some campaign programs designed to reduce anti-social behaviour seek to elicit empathy for the victims. The effectiveness of these campaigns can be evaluated according to the degree to which they induce such responses, but by applying neuroscientific techniques this can be done at the behavioural and neurophysiological level. Neuroimaging studies aimed at identifying the neural mechanisms behind such socio-cognitive and -emotional processes frequently reveal the role of the superior temporal sulcus (STS). We applied this knowledge to assess the effectiveness of traffic-awareness campaign adverts to induce empathic expression. Functional magnetic resonance imaging (fMRI) data were acquired from 20 healthy male volunteers as they watched these campaign videos consisting of a dramatic sequence of events and catastrophic endings, and control videos without such dramatic endings. Among other structures, a significantly greater neural response was observed within bilateral STS, particularly within the right hemisphere, during the observation of campaign relative to control videos. Furthermore, activation in these brain regions correlated with the subjects’ empathic expression. Our results develop our understanding of the role of STS in social cognition. Moreover, our data demonstrate the utility of neuroscientific methods when evaluating the effectiveness of campaign videos in terms of their ability to elicit empathic responses. Our study also demonstrates the utility of these specific stimuli for future neuroscientific research. PMID:25118071

Characterization of Apoptosis Signaling Cascades During the Differentiation Process of Human Neural ReNcell VM Progenitor Cells In Vitro.

PubMed

Jaeger, Alexandra; Fröhlich, Michael; Klum, Susanne; Lantow, Margareta; Viergutz, Torsten; Weiss, Dieter G; Kriehuber, Ralf

2015-11-01

Apoptosis is an essential physiological process accompanying the development of the central nervous system and human neurogenesis. However, the time scale and the underlying molecular mechanisms are yet poorly understood. Due to this fact, we investigated the functionality and general inducibility of apoptosis in the human neural ReNcell VM progenitor cell line during differentiation and also after exposure to staurosporine (STS) and ultraviolet B (UVB) irradiation. Transmission light microscopy, flow cytometry, and Western-/Immunoblot analysis were performed to compare proliferating and differentiating, in addition to STS- and UVB-treated cells. In particular, from 24 to 72 h post-initiation of differentiation, G0/G1 cell cycle arrest, increased loss of apoptotic cells, activation of pro-apoptotic BAX, Caspase-3, and cleavage of its substrate PARP were observed during cell differentiation and, to a higher extent, after treatment with STS and UVB. We conclude that redundant or defective cells are eliminated by apoptosis, while otherwise fully differentiated cells were less responsive to apoptosis induction by STS than proliferating cells, likely as a result of reduced APAF-1 expression, and increased levels of BCL-2. These data provide the evidence that apoptotic mechanisms in the neural ReNcell VM progenitor cell line are not only functional, but also inducible by external stimuli like growth factor withdrawal or treatment with STS and UVB, which marks this cell line as a suitable model to investigate apoptosis signaling pathways in respect to the differentiation processes of human neural progenitor cells in vitro.

A stilbene synthase allele from a Chinese wild grapevine confers resistance to powdery mildew by recruiting salicylic acid signalling for efficient defence.

PubMed

Jiao, Yuntong; Xu, Weirong; Duan, Dong; Wang, Yuejin; Nick, Peter

2016-10-01

Stilbenes are central phytoalexins in Vitis, and induction of the key enzyme stilbene synthase (STS) is pivotal for disease resistance. Here, we address the potential for breeding resistance using an STS allele isolated from Chinese wild grapevine Vitis pseudoreticulata (VpSTS) by comparison with its homologue from Vitis vinifera cv. 'Carigane' (VvSTS). Although the coding regions of both alleles are very similar (>99% identity on the amino acid level), the promoter regions are significantly different. By expression in Arabidopsis as a heterologous system, we show that the allele from the wild Chinese grapevine can confer accumulation of stilbenes and resistance against the powdery mildew Golovinomyces cichoracearum, whereas the allele from the vinifera cultivar cannot. To dissect the upstream signalling driving the activation of this promoter, we used a dual-luciferase reporter system in a grapevine cell culture. We show elevated responsiveness of the promoter from the wild grape to salicylic acid (SA) and to the pathogen-associated molecular pattern (PAMP) flg22, equal induction of both alleles by jasmonic acid (JA), and a lack of response to the cell death-inducing elicitor Harpin. This elevated SA response of the VpSTS promoter depends on calcium influx, oxidative burst by RboH, mitogen-activated protein kinase (MAPK) signalling, and JA synthesis. We integrate the data in the context of a model where the resistance of V. pseudoreticulata is linked to a more efficient recruitment of SA signalling for phytoalexin synthesis. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Comparison and Evaluation of the Molecular Typing Methods for Toxigenic Vibrio cholerae in Southwest China.

PubMed

Liao, Feng; Mo, Zhishuo; Chen, Meiling; Pang, Bo; Fu, Xiaoqing; Xu, Wen; Jing, Huaiqi; Kan, Biao; Gu, Wenpeng

2018-01-01

Vibrio cholerae O1 strains taken from the repository of Yunnan province, southwest China, were abundant and special. We selected 70 typical toxigenic V. cholerae (69 O1 and one O139 serogroup strains) isolated from Yunnan province, performed the pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and MLST of virulence gene (V-MLST) methods, and evaluated the resolution abilities for typing methods. The ctxB subunit sequence analysis for all strains have shown that cholera between 1986 and 1995 was associated with mixed infections with El Tor and El Tor variants, while infections after 1996 were all caused by El Tor variant strains. Seventy V. cholerae obtained 50 PFGE patterns, with a high resolution. The strains could be divided into three groups with predominance of strains isolated during 1980s, 1990s, and 2000s, respectively, showing a good consistency with the epidemiological investigation. We also evaluated two MLST method for V. cholerae , one was used seven housekeeping genes ( adk , gyrB , metE , pntA , mdh , purM , and pyrC ), and all the isolates belonged to ST69; another was used nine housekeeping genes ( cat , chi , dnaE , gyrB , lap , pgm , recA , rstA , and gmd ). A total of seven sequence types (STs) were found by using this method for all the strains; among them, rstA gene had five alleles, recA and gmd have two alleles, and others had only one allele. The virulence gene sequence typing method ( ctxAB , tcpA , and toxR ) showed that 70 strains were divided into nine STs; among them, tcpA gene had six alleles, toxR had five alleles, while ctxAB was identical for all the strains. The latter two sequences based typing methods also had consistency with epidemiology of the strains. PFGE had a higher resolution ability compared with the sequence based typing method, and MLST used seven housekeeping genes showed the lower resolution power than nine housekeeping genes and virulence genes methods. These two sequence typing methods could distinguish some epidemiological special strains in local area.

FARO server: Meta-analysis of gene expression by matching gene expression signatures to a compendium of public gene expression data.

PubMed

Manijak, Mieszko P; Nielsen, Henrik B

2011-06-11

Although, systematic analysis of gene annotation is a powerful tool for interpreting gene expression data, it sometimes is blurred by incomplete gene annotation, missing expression response of key genes and secondary gene expression responses. These shortcomings may be partially circumvented by instead matching gene expression signatures to signatures of other experiments. To facilitate this we present the Functional Association Response by Overlap (FARO) server, that match input signatures to a compendium of 242 gene expression signatures, extracted from more than 1700 Arabidopsis microarray experiments. Hereby we present a publicly available tool for robust characterization of Arabidopsis gene expression experiments which can point to similar experimental factors in other experiments. The server is available at http://www.cbs.dtu.dk/services/faro/.

Digital gene expression analysis of the zebra finch genome

PubMed Central

2010-01-01

Background In order to understand patterns of adaptation and molecular evolution it is important to quantify both variation in gene expression and nucleotide sequence divergence. Gene expression profiling in non-model organisms has recently been facilitated by the advent of massively parallel sequencing technology. Here we investigate tissue specific gene expression patterns in the zebra finch (Taeniopygia guttata) with special emphasis on the genes of the major histocompatibility complex (MHC). Results Almost 2 million 454-sequencing reads from cDNA of six different tissues were assembled and analysed. A total of 11,793 zebra finch transcripts were represented in this EST data, indicating a transcriptome coverage of about 65%. There was a positive correlation between the tissue specificity of gene expression and non-synonymous to synonymous nucleotide substitution ratio of genes, suggesting that genes with a specialised function are evolving at a higher rate (or with less constraint) than genes with a more general function. In line with this, there was also a negative correlation between overall expression levels and expression specificity of contigs. We found evidence for expression of 10 different genes related to the MHC. MHC genes showed relatively tissue specific expression levels and were in general primarily expressed in spleen. Several MHC genes, including MHC class I also showed expression in brain. Furthermore, for all genes with highest levels of expression in spleen there was an overrepresentation of several gene ontology terms related to immune function. Conclusions Our study highlights the usefulness of next-generation sequence data for quantifying gene expression in the genome as a whole as well as in specific candidate genes. Overall, the data show predicted patterns of gene expression profiles and molecular evolution in the zebra finch genome. Expression of MHC genes in particular, corresponds well with expression patterns in other vertebrates. PMID:20359325

A novel approach for human whole transcriptome analysis based on absolute gene expression of microarray data.

PubMed

Bikel, Shirley; Jacobo-Albavera, Leonor; Sánchez-Muñoz, Fausto; Cornejo-Granados, Fernanda; Canizales-Quinteros, Samuel; Soberón, Xavier; Sotelo-Mundo, Rogerio R; Del Río-Navarro, Blanca E; Mendoza-Vargas, Alfredo; Sánchez, Filiberto; Ochoa-Leyva, Adrian

2017-01-01

In spite of the emergence of RNA sequencing (RNA-seq), microarrays remain in widespread use for gene expression analysis in the clinic. There are over 767,000 RNA microarrays from human samples in public repositories, which are an invaluable resource for biomedical research and personalized medicine. The absolute gene expression analysis allows the transcriptome profiling of all expressed genes under a specific biological condition without the need of a reference sample. However, the background fluorescence represents a challenge to determine the absolute gene expression in microarrays. Given that the Y chromosome is absent in female subjects, we used it as a new approach for absolute gene expression analysis in which the fluorescence of the Y chromosome genes of female subjects was used as the background fluorescence for all the probes in the microarray. This fluorescence was used to establish an absolute gene expression threshold, allowing the differentiation between expressed and non-expressed genes in microarrays. We extracted the RNA from 16 children leukocyte samples (nine males and seven females, ages 6-10 years). An Affymetrix Gene Chip Human Gene 1.0 ST Array was carried out for each sample and the fluorescence of 124 genes of the Y chromosome was used to calculate the absolute gene expression threshold. After that, several expressed and non-expressed genes according to our absolute gene expression threshold were compared against the expression obtained using real-time quantitative polymerase chain reaction (RT-qPCR). From the 124 genes of the Y chromosome, three genes (DDX3Y, TXLNG2P and EIF1AY) that displayed significant differences between sexes were used to calculate the absolute gene expression threshold. Using this threshold, we selected 13 expressed and non-expressed genes and confirmed their expression level by RT-qPCR. Then, we selected the top 5% most expressed genes and found that several KEGG pathways were significantly enriched. Interestingly, these pathways were related to the typical functions of leukocytes cells, such as antigen processing and presentation and natural killer cell mediated cytotoxicity. We also applied this method to obtain the absolute gene expression threshold in already published microarray data of liver cells, where the top 5% expressed genes showed an enrichment of typical KEGG pathways for liver cells. Our results suggest that the three selected genes of the Y chromosome can be used to calculate an absolute gene expression threshold, allowing a transcriptome profiling of microarray data without the need of an additional reference experiment. Our approach based on the establishment of a threshold for absolute gene expression analysis will allow a new way to analyze thousands of microarrays from public databases. This allows the study of different human diseases without the need of having additional samples for relative expression experiments.

Differential gene expression analysis in glioblastoma cells and normal human brain cells based on GEO database.

PubMed

Wang, Anping; Zhang, Guibin

2017-11-01

The differentially expressed genes between glioblastoma (GBM) cells and normal human brain cells were investigated to performed pathway analysis and protein interaction network analysis for the differentially expressed genes. GSE12657 and GSE42656 gene chips, which contain gene expression profile of GBM were obtained from Gene Expression Omniub (GEO) database of National Center for Biotechnology Information (NCBI). The 'limma' data packet in 'R' software was used to analyze the differentially expressed genes in the two gene chips, and gene integration was performed using 'RobustRankAggreg' package. Finally, pheatmap software was used for heatmap analysis and Cytoscape, DAVID, STRING and KOBAS were used for protein-protein interaction, Gene Ontology (GO) and KEGG analyses. As results: i) 702 differentially expressed genes were identified in GSE12657, among those genes, 548 were significantly upregulated and 154 were significantly downregulated (p<0.01, fold-change >1), and 1,854 differentially expressed genes were identified in GSE42656, among the genes, 1,068 were significantly upregulated and 786 were significantly downregulated (p<0.01, fold-change >1). A total of 167 differentially expressed genes including 100 upregulated genes and 67 downregulated genes were identified after gene integration, and the genes showed significantly different expression levels in GBM compared with normal human brain cells (p<0.05). ii) Interactions between the protein products of 101 differentially expressed genes were identified using STRING and expression network was established. A key gene, called CALM3, was identified by Cytoscape software. iii) GO enrichment analysis showed that differentially expressed genes were mainly enriched in 'neurotransmitter:sodium symporter activity' and 'neurotransmitter transporter activity', which can affect the activity of neurotransmitter transportation. KEGG pathway analysis showed that the differentially expressed genes were mainly enriched in 'protein processing in endoplasmic reticulum', which can affect protein processing in endoplasmic reticulum. The results showed that: i) 167 differentially expressed genes were identified from two gene chips after integration; and ii) protein interaction network was established, and GO and KEGG pathway analyses were successfully performed to identify and annotate the key gene, which provide new insights for the studies on GBN at gene level.

Discovery and validation of a glioblastoma co-expressed gene module

PubMed Central

Dunwoodie, Leland J.; Poehlman, William L.; Ficklin, Stephen P.; Feltus, Frank Alexander

2018-01-01

Tumors exhibit complex patterns of aberrant gene expression. Using a knowledge-independent, noise-reducing gene co-expression network construction software called KINC, we created multiple RNAseq-based gene co-expression networks relevant to brain and glioblastoma biology. In this report, we describe the discovery and validation of a glioblastoma-specific gene module that contains 22 co-expressed genes. The genes are upregulated in glioblastoma relative to normal brain and lower grade glioma samples; they are also hypo-methylated in glioblastoma relative to lower grade glioma tumors. Among the proneural, neural, mesenchymal, and classical glioblastoma subtypes, these genes are most-highly expressed in the mesenchymal subtype. Furthermore, high expression of these genes is associated with decreased survival across each glioblastoma subtype. These genes are of interest to glioblastoma biology and our gene interaction discovery and validation workflow can be used to discover and validate co-expressed gene modules derived from any co-expression network. PMID:29541392

Discovery and validation of a glioblastoma co-expressed gene module.

PubMed

Dunwoodie, Leland J; Poehlman, William L; Ficklin, Stephen P; Feltus, Frank Alexander

2018-02-16

Tumors exhibit complex patterns of aberrant gene expression. Using a knowledge-independent, noise-reducing gene co-expression network construction software called KINC, we created multiple RNAseq-based gene co-expression networks relevant to brain and glioblastoma biology. In this report, we describe the discovery and validation of a glioblastoma-specific gene module that contains 22 co-expressed genes. The genes are upregulated in glioblastoma relative to normal brain and lower grade glioma samples; they are also hypo-methylated in glioblastoma relative to lower grade glioma tumors. Among the proneural, neural, mesenchymal, and classical glioblastoma subtypes, these genes are most-highly expressed in the mesenchymal subtype. Furthermore, high expression of these genes is associated with decreased survival across each glioblastoma subtype. These genes are of interest to glioblastoma biology and our gene interaction discovery and validation workflow can be used to discover and validate co-expressed gene modules derived from any co-expression network.

Gender-Specific Gene Expression in Post-Mortem Human Brain: Localization to Sex Chromosomes

PubMed Central

Vawter, Marquis P; Evans, Simon; Choudary, Prabhakara; Tomita, Hiroaki; Meador-Woodruff, Jim; Molnar, Margherita; Li, Jun; Lopez, Juan F; Myers, Rick; Cox, David; Watson, Stanley J; Akil, Huda; Jones, Edward G; Bunney, William E

2011-01-01

Gender differences in brain development and in the prevalence of neuropsychiatric disorders such as depression have been reported. Gender differences in human brain might be related to patterns of gene expression. Microarray technology is one useful method for investigation of gene expression in brain. We investigated gene expression, cell types, and regional expression patterns of differentially expressed sex chromosome genes in brain. We profiled gene expression in male and female dorsolateral prefrontal cortex, anterior cingulate cortex, and cerebellum using the Affymetrix oligonucleotide microarray platform. Differentially expressed genes between males and females on the Y chromosome (DBY, SMCY, UTY, RPS4Y, and USP9Y) and X chromosome (XIST) were confirmed using real-time PCR measurements. In situ hybridization confirmed the differential expression of gender-specific genes and neuronal expression of XIST, RPS4Y, SMCY, and UTY in three brain regions examined. The XIST gene, which silences gene expression on regions of the X chromosome, is expressed in a subset of neurons. Since a subset of neurons express gender-specific genes, neural subpopulations may exhibit a subtle sexual dimorphism at the level of differences in gene regulation and function. The distinctive pattern of neuronal expression of XIST, RPS4Y, SMCY, and UTY and other sex chromosome genes in neuronal subpopulations may possibly contribute to gender differences in prevalence noted for some neuropsychiatric disorders. Studies of the protein expression of these sex- chromosome-linked genes in brain tissue are required to address the functional consequences of the observed gene expression differences. PMID:14583743

KSC-2011-2040

NASA Image and Video Library

2011-03-02

CAPE CANAVERAL, Fla. -- Space shuttle Endeavour is firmly attached to its external fuel tank and solid rocket boosters in a high bay of the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida. Endeavour is targeted to roll out to Kennedy's Launch Pad 39A for its final mission, STS-134, on March 9. Endeavour and the six-member crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank, additional spare parts for the Dextre robotic helper and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Kim Shiflett

KSC-2011-1972

NASA Image and Video Library

2011-03-01

CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, shuttle Endeavour is suspended above the transfer aisle. The spacecraft will be lifted and moved into a high bay where it will be attached to its external fuel tank and solid rocket boosters, already positioned on the mobile launcher platform. Endeavour and its STS-134 crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer, a high-pressure gas tank, additional spare parts for Dextre and micrometeoroid debris shields to the International Space Station. Endeavour's final launch is targeted for April 19 at 7:48 p.m. EDT. For more information visit, http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jim Grossmann

Microbial survival in space shuttle crash

PubMed Central

McLean, Robert J.C.; Welsh, Allana K.; Casasanto, Valerie A.

2011-01-01

A slow growing, heat resistant bacterium, identified by 16S rRNA gene sequencing as Microbispora sp., was recovered from the wreckage of the ill-fated space shuttle Columbia (STS-107). As this organism survived disintegration of the space craft, heat of reentry, and impact, it supports the possibility of a natural mechanism for the interplanetary spread of life by meteorites. PMID:21804644

Gene expression variability in human hepatic drug metabolizing enzymes and transporters.

PubMed

Yang, Lun; Price, Elvin T; Chang, Ching-Wei; Li, Yan; Huang, Ying; Guo, Li-Wu; Guo, Yongli; Kaput, Jim; Shi, Leming; Ning, Baitang

2013-01-01

Interindividual variability in the expression of drug-metabolizing enzymes and transporters (DMETs) in human liver may contribute to interindividual differences in drug efficacy and adverse reactions. Published studies that analyzed variability in the expression of DMET genes were limited by sample sizes and the number of genes profiled. We systematically analyzed the expression of 374 DMETs from a microarray data set consisting of gene expression profiles derived from 427 human liver samples. The standard deviation of interindividual expression for DMET genes was much higher than that for non-DMET genes. The 20 DMET genes with the largest variability in the expression provided examples of the interindividual variation. Gene expression data were also analyzed using network analysis methods, which delineates the similarities of biological functionalities and regulation mechanisms for these highly variable DMET genes. Expression variability of human hepatic DMET genes may affect drug-gene interactions and disease susceptibility, with concomitant clinical implications.

AVGS, AR and D for Satellites, ISS, the Moon, Mars and Beyond

NASA Technical Reports Server (NTRS)

Hintze, Geoffrey C.; Cornett, Keith G.; Rahmatipour, Michael H.; Heaton, Andrew F.; Newman, Larry E.; Fleischmann, Kevin D.; Hamby, Byron J.

2007-01-01

With the continuous need to rotate crew and re-supply the International Space Station (ISS) and the desire to return humans to the Moon and for the first time, place humans on Mars, NASA must develop a more robust and highly reliable capability to perform Autonomous Rendezvous and Capture (AR&C) because, unlike the Apollo missions, NASA plans to send the entire crew to the Lunar or Martian surface and must be able to dock with the Orion spacecraft upon return. In 1997, NASA developed the Video Guidance Sensor (VGS) which was flown and tested on STS-87 and STS-95. In 2001, NASA designed and built a more enhanced version of the VGS, called the Advanced Video Guidance Sensor (AVGS). The AVGS offered significant technology improvements to the precursor VGS design. This paper will describe the AVGS as it was in the DART mission of 2005 and the Orbital Express mission of 2007. The paper will describe the capabilities and design concepts of the AVGS as it was flown on the DART 2005 Mission and the DARPA Orbital Express Mission slated to fly in 2007. The paper will cover the Flight Software, problems encountered, testing for Orbital Express and where NASA is going in the future.

A novel approach for human whole transcriptome analysis based on absolute gene expression of microarray data

PubMed Central

Bikel, Shirley; Jacobo-Albavera, Leonor; Sánchez-Muñoz, Fausto; Cornejo-Granados, Fernanda; Canizales-Quinteros, Samuel; Soberón, Xavier; Sotelo-Mundo, Rogerio R.; del Río-Navarro, Blanca E.; Mendoza-Vargas, Alfredo; Sánchez, Filiberto

2017-01-01

Background In spite of the emergence of RNA sequencing (RNA-seq), microarrays remain in widespread use for gene expression analysis in the clinic. There are over 767,000 RNA microarrays from human samples in public repositories, which are an invaluable resource for biomedical research and personalized medicine. The absolute gene expression analysis allows the transcriptome profiling of all expressed genes under a specific biological condition without the need of a reference sample. However, the background fluorescence represents a challenge to determine the absolute gene expression in microarrays. Given that the Y chromosome is absent in female subjects, we used it as a new approach for absolute gene expression analysis in which the fluorescence of the Y chromosome genes of female subjects was used as the background fluorescence for all the probes in the microarray. This fluorescence was used to establish an absolute gene expression threshold, allowing the differentiation between expressed and non-expressed genes in microarrays. Methods We extracted the RNA from 16 children leukocyte samples (nine males and seven females, ages 6–10 years). An Affymetrix Gene Chip Human Gene 1.0 ST Array was carried out for each sample and the fluorescence of 124 genes of the Y chromosome was used to calculate the absolute gene expression threshold. After that, several expressed and non-expressed genes according to our absolute gene expression threshold were compared against the expression obtained using real-time quantitative polymerase chain reaction (RT-qPCR). Results From the 124 genes of the Y chromosome, three genes (DDX3Y, TXLNG2P and EIF1AY) that displayed significant differences between sexes were used to calculate the absolute gene expression threshold. Using this threshold, we selected 13 expressed and non-expressed genes and confirmed their expression level by RT-qPCR. Then, we selected the top 5% most expressed genes and found that several KEGG pathways were significantly enriched. Interestingly, these pathways were related to the typical functions of leukocytes cells, such as antigen processing and presentation and natural killer cell mediated cytotoxicity. We also applied this method to obtain the absolute gene expression threshold in already published microarray data of liver cells, where the top 5% expressed genes showed an enrichment of typical KEGG pathways for liver cells. Our results suggest that the three selected genes of the Y chromosome can be used to calculate an absolute gene expression threshold, allowing a transcriptome profiling of microarray data without the need of an additional reference experiment. Discussion Our approach based on the establishment of a threshold for absolute gene expression analysis will allow a new way to analyze thousands of microarrays from public databases. This allows the study of different human diseases without the need of having additional samples for relative expression experiments. PMID:29230367

Genotypes, antibiotic resistance, and virulence factors of staphylococci from ready-to-eat food.

PubMed

Podkowik, Magdalena; Bystroń, Jarosław; Bania, Jacek

2012-01-01

Sixty-seven staphylococcal isolates belonging to 12 species were obtained from 70 ready-to-eat food products. Staphylococcus aureus (n=25), and Staphylococcus epidermidis (n=13) were dominant. Susceptibility to penicillin, oxacillin, tetracycline, clindamycin, gentamicin, erythromycin, ciprofloxacin, and vancomycin was determined. All investigated S. aureus isolates were resistant to at least one antibiotic, and fifteen isolates were resistant to four and more antibiotics. Thirty-eight coagulase-negative staphylococci (CNS) isolates were resistant to at least one antibiotic, and seventeen to four and more antibiotics. Fifteen CNS isolates were mecA positive, and grew in the presence of 6 μg/mL oxacillin. All S. aureus isolates were mecA-negative. Arginine catabolic mobile element (ACME) was found in seven S. epidermidis isolates. Five S. epidermidis isolates harbored ica operon, ACME and were able to form biofilm. Three of them also possessed IS256 element and were mecA-positive. The expression of icaA gene was comparable in five ica-positive S. epidermidis isolates. One of six mecA positive S. epidermidis isolates was classified as sequence type (ST)155, one as ST110, and two as ST88. Two methicillin-resistant Staphylococcus epidermis (MRSE) belonged to new STs, that is, ST362, and ST363. Enterotoxin genes were found in 92% of S. aureus isolates. No enterotoxin gene was detected in analyzed CNS population. We show that ready-to-eat products are an important source of antibiotic-resistant CNS and potentially virulent strains of S. epidermidis, including genotypes undistinguishable from hospital-adapted clones.

KSC-2011-4185

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Xenon lights illuminate space shuttle Endeavour's unfurled drag chute as the vehicle rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. On board are STS-134 Commander Mark Kelly, Pilot Greg H. Johnson, and Mission Specialists Mike Fincke, Drew Feustel, Greg Chamitoff and the European Space Agency's Roberto Vittori. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/George Roberto

KSC-2011-4178

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Xenon lights illuminate space shuttle Endeavour's unfurled drag chute as the vehicle rolls to a stop on the Shuttle Landing Facility's Runway 15 at NASA's Kennedy Space Center in Florida for the final time. Main gear touchdown was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. On board are STS-134 Commander Mark Kelly, Pilot Greg H. Johnson, and Mission Specialists Mike Fincke, Drew Feustel, Greg Chamitoff and the European Space Agency's Roberto Vittori. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kenny Allen