Temperature mapping of laser-induced hyperthermia in an ocular phantom using magnetic resonance thermography.

PubMed

Maswadi, Saher M; Dodd, Stephen J; Gao, Jia-Hong; Glickman, Randolph D

2004-01-01

Laser-induced heating in an ocular phantom is measured with magnetic resonance thermography (MRT) using temperature-dependent phase changes in proton resonance frequency. The ocular phantom contains a layer of melanosomes isolated from bovine retinal pigment epithelium. The phantom is heated by the 806-nm output of a continuous wave diode laser with an irradiance of 2.4 to 21.6 W/cm2 in a beam radius of 0.8 or 2.4 mm, depending on the experiment. MRT is performed with a 2 T magnet, and a two-turn, 6-cm-diam, circular radio frequency coil. Two-dimensional temperature gradients are measured within the plane of the melanin layer, as well as normal to it, with a temperature resolution of 1 degrees C or better. The temperature gradients extending within the melanin layer are broader than those orthogonal to the layer, consistent with the higher optical absorption and consequent heating in the melanin. The temperature gradients in the phantom measured by MRT closely approximate the predictions of a classical heat diffusion model. Three-dimensional temperature maps with a spatial resolution of 0.25 mm in all directions are also made. Although the temporal resolution is limited in the prototype system (22.9 s for a single image "slice"), improvements in future implementations are likely. These results indicate that MRT has sufficient spatial and temperature resolution to monitor target tissue temperature during transpupillary thermotherapy in the human eye.

Ultrafast two-dimensional lithium beam emission spectroscopy diagnostic on the EAST tokamak

NASA Astrophysics Data System (ADS)

Zoletnik, S.; Hu, G. H.; Tál, B.; Dunai, D.; Anda, G.; Asztalos, O.; Pokol, G. I.; Kálvin, S.; Németh, J.; Krizsanóczi, T.

2018-06-01

A diagnostic instrument is described for the Experimental Advanced Superconducting Tokamak (EAST) for the measurement of the edge plasma electron density profile and plasma turbulence properties. An accelerated neutral lithium beam is injected into the tokamak and the Doppler shifted 670.8 nm light emission of the Li2p-2s transition is detected. A novel compact setup is used, where the beam injection and observation take place from the same equatorial diagnostic port and radial-poloidal resolution is achieved with microsecond time resolution. The observation direction is optimized in order to achieve a sufficient Doppler shift of the beam light to be able to separate from the strong edge lithium line emission on this lithium coated device. A 250 kHz beam chopping technique is also demonstrated for the removal of background light. First results show the capability of measuring turbulence and its poloidal flow velocity in the scrape-off layer and edge region and the resolution of details of transient phenomena like edge localized modes with few microsecond time resolution.

Cerebral cortex three-dimensional profiling in human fetuses by magnetic resonance imaging

PubMed Central

Sbarbati, Andrea; Pizzini, Francesca; Fabene, Paolo F; Nicolato, Elena; Marzola, Pasquina; Calderan, Laura; Simonati, Alessandro; Longo, Laura; Osculati, Antonio; Beltramello, Alberto

2004-01-01

Seven human fetuses of crown/rump length corresponding to gestational ages ranging from the 12th to the 16th week were studied using a paradigm based on three-dimensional reconstruction of the brain obtained by magnetic resonance imaging (MRI). The aim of the study was to evaluate brain morphology in situ and to describe developmental dynamics during an important period of fetal morphogenesis. Three-dimensional MRI showed the increasing degree of maturation of the brains; fronto-occipital distance, bitemporal distance and occipital angle were examined in all the fetuses. The data were interpreted by correlation with the internal structure as visualized using high-spatial-resolution MRI, acquired using a 4.7-T field intensity magnet with a gradient power of 20 G cm−1. The spatial resolution was sufficient for a detailed detection of five layers, and the contrast was optimized using sequences with different degrees of T1 and T2 weighting. Using the latter, it was possible to visualize the subplate and marginal zones. The cortical thickness was mapped on to the hemispheric surface, describing the thickness gradient from the insular cortex to the periphery of the hemispheres. The study demonstrates the utility of MRI for studying brain development. The method provides a quantitative profiling of the brain, which allows the calculation of important morphological parameters, and it provides informative regarding transient features of the developing brain. PMID:15198688

[4D-MRI using the synchronized sampling method (SSM)].

PubMed

Shimada, Yasuhiro; Fujimoto, Ichirou; Takemoto, Hironori; Takano, Sayoko; Masaki, Shinobu; Honda, Kiyoshi; Takeo, Kazuhiro

2002-12-01

A synchronized sampling method (SSM) was developed for the study of voluntary movements by combining the electrocardiographic (ECG) gating method with an external triggering device, and four-dimensional magnetic resonance imaging (4D-MRI) at a rate of 30 frames per second was accomplished by volumetric imaging with the SSM. This method was first applied to the motion imaging of articulatory organs during repetitions of a Japanese five-vowel sequence, and the dynamic change in vocal tract area function was demonstrated with sufficient temporal resolution. This paper describes the methodology, applicability, and limitations of 4D-MRI with the SSM.

The development of laser speckle velocimetry for the measurement of vortical flow fields

NASA Technical Reports Server (NTRS)

Smith, C. A.; Lourenco, L. M. M.; Krothapalli, A.

1986-01-01

A new velocity measurement technique is described that provides the simultaneous visualization of a two-dimensional streamline pattern and the quantification of the velocity field. The main advantage of this technique is that the velocity field can be measured with sufficient accuracy and spatial resolution so that the vorticity field can be readily obtained. This technique is ideally suited for the study of unsteady vortical flows, which occur in rotorcraft and in high-angle-of-attack aerodynamics. The technique, some of the important parameters that affect its use, and some recent examples are described.

Dehydration and clearing of adult Drosophila for ultramicroscopy.

PubMed

Becker, Klaus; Jährling, Nina; Saghafi, Saiedeh; Dodt, Hans-Ulrich

2013-07-01

This protocol describes the preparation of adult flies for ultramicroscopy (UM), a powerful imaging technique that achieves precise and accurate three-dimensional (3D) reconstructions of intact macroscopic specimens with micrometer resolution. In UM, a specimen in the size range of ∼1-15 mm is illuminated perpendicular to the observation pathway by two thin counterpropagating sheets of laser light. Thus, specimens for UM need to be sufficiently transparent, which requires chemical clearing in most cases. In this protocol, Drosophila melanogaster adults are fixed, dehydrated in ethanol, and then cleared in a solution of benzyl alcohol and benzyl benzoate.

GFZ Wireless Seismic Array (GFZ-WISE), a Wireless Mesh Network of Seismic Sensors: New Perspectives for Seismic Noise Array Investigations and Site Monitoring

PubMed Central

Picozzi, Matteo; Milkereit, Claus; Parolai, Stefano; Jaeckel, Karl-Heinz; Veit, Ingo; Fischer, Joachim; Zschau, Jochen

2010-01-01

Over the last few years, the analysis of seismic noise recorded by two dimensional arrays has been confirmed to be capable of deriving the subsoil shear-wave velocity structure down to several hundred meters depth. In fact, using just a few minutes of seismic noise recordings and combining this with the well known horizontal-to-vertical method, it has also been shown that it is possible to investigate the average one dimensional velocity structure below an array of stations in urban areas with a sufficient resolution to depths that would be prohibitive with active source array surveys, while in addition reducing the number of boreholes required to be drilled for site-effect analysis. However, the high cost of standard seismological instrumentation limits the number of sensors generally available for two-dimensional array measurements (i.e., of the order of 10), limiting the resolution in the estimated shear-wave velocity profiles. Therefore, new themes in site-effect estimation research by two-dimensional arrays involve the development and application of low-cost instrumentation, which potentially allows the performance of dense-array measurements, and the development of dedicated signal-analysis procedures for rapid and robust estimation of shear-wave velocity profiles. In this work, we present novel low-cost wireless instrumentation for dense two-dimensional ambient seismic noise array measurements that allows the real–time analysis of the surface-wavefield and the rapid estimation of the local shear-wave velocity structure for site response studies. We first introduce the general philosophy of the new system, as well as the hardware and software that forms the novel instrument, which we have tested in laboratory and field studies. PMID:22319298

Method of fabricating free-form, high-aspect ratio components for high-current, high-speed microelectrics

DOEpatents

Maxwell, James L; Rose, Chris R; Black, Marcie R; Springer, Robert W

2014-03-11

Microelectronic structures and devices, and method of fabricating a three-dimensional microelectronic structure is provided, comprising passing a first precursor material for a selected three-dimensional microelectronic structure into a reaction chamber at temperatures sufficient to maintain said precursor material in a predominantly gaseous state; maintaining said reaction chamber under sufficient pressures to enhance formation of a first portion of said three-dimensional microelectronic structure; applying an electric field between an electrode and said microelectronic structure at a desired point under conditions whereat said first portion of a selected three-dimensional microelectronic structure is formed from said first precursor material; positionally adjusting either said formed three-dimensional microelectronic structure or said electrode whereby further controlled growth of said three-dimensional microelectronic structure occurs; passing a second precursor material for a selected three-dimensional microelectronic structure into a reaction chamber at temperatures sufficient to maintain said precursor material in a predominantly gaseous state; maintaining said reaction chamber under sufficient pressures whereby a second portion of said three-dimensional microelectronic structure formation is enhanced; applying an electric field between an electrode and said microelectronic structure at a desired point under conditions whereat said second portion of a selected three-dimensional microelectronic structure is formed from said second precursor material; and, positionally adjusting either said formed three-dimensional microelectronic structure or said electrode whereby further controlled growth of said three-dimensional microelectronic structure occurs.

Navier-Stokes Simulation of UH-60A Rotor/Wake Interaction Using Adaptive Mesh Refinement

NASA Technical Reports Server (NTRS)

Chaderjian, Neal M.

2017-01-01

High-resolution simulations of rotor/vortex-wake interaction for a UH60-A rotor under BVI and dynamic stallconditions were carried out with the OVERFLOW Navier-Stokes code.a. The normal force and pitching moment variation with azimuth angle were in good overall agreementwith flight-test data, similar to other CFD results reported in the literature.b. The wake-grid resolution did not have a significant effect on the rotor-blade airloads. This surprisingresult indicates that a wake grid spacing of (Delta)S=10% ctip is sufficient for engineering airloads predictionfor hover and forward flight. This assumes high-resolution body grids, high-order spatial accuracy, anda hybrid RANS/DDES turbulence model.c. Three-dimensional dynamic stall was found to occur due the presence of blade-tip vortices passing overa rotor blade on the retreating side. This changed the local airfoil angle of attack, causing stall, unlikethe 2D perspective of pure pitch oscillation of the local airfoil section.

Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy

NASA Astrophysics Data System (ADS)

Zhang, Kai; Li, De-E.; Hong, You-Li; Zhu, Pei-Ping; Yuan, Qing-Xi; Huang, Wan-Xia; Gao, Kun; Zhou, Hong-Zhang; Wu, Zi-Yu

2013-07-01

A penetrating view of the three-dimensional nanostructure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscopy are quite promising for insect morphology studies and, particularly, provide an attractive alternative to the destructive techniques used for investigating internal soft tissues.

A new high-resolution PET scanner dedicated to brain research

NASA Astrophysics Data System (ADS)

Watanabe, M.; Shimizu, K.; Omura, T.; Takahashi, M.; Kosugi, T.; Yoshikawa, E.; Sato, N.; Okada, H.; Yamashita, T.

2002-06-01

A high-resolution positron emission tomography (PET) scanner dedicated to brain studies has been developed and its physical performance was evaluated. The block detector consists of a new compact position-sensitive photomultiplier tube (PS-PMT, Hamamatsu R7600-C12) and an 8/spl times/4 bismuth germanate (BGO) array. The size of each crystal is 2.8 mm/spl times/6.55 mm/spl times/30 mm. The system has a total of 11 520 crystals arranged in 24 detector rings 508 mm in diameter (480 per ring). The field of view (FOV) is 330 mm in diameter/spl times/163 mm, which is sufficient to measure the entire human brain. The diameter of the scanner's opening is equal to the transaxial FOV (330 mm). The system can be operated in three-dimensional (3-D) data acquisition mode, when the slice septa are retracted. The mechanical motions of the gantry and bed are specially designed to measure the patient in various postures; lying, sitting, and even standing postures. The spatial resolution of 2.9 mm in both the transaxial and axial directions is obtained at the center of the FOV. The total system sensitivity is 6.4 kc/s/kBq/ml in two-dimensional (2-D) mode, with a 20-cm-diameter cylindrical phantom. The imaging capabilities of the scanner were studied with the Hoffman brain phantom and with a normal volunteer.

Confocal reflectance quantitative phase microscope system for cellular membranes dynamics study (Conference Presentation)

NASA Astrophysics Data System (ADS)

Singh, Vijay Raj; Yaqoob, Zahid; So, Peter T. C.

2017-02-01

Quantitative phase microscopy (QPM) techniques developed so far primarily belongs to high speed transmitted light based systems that has enough sensitivity to resolve membrane fluctuations and dynamics, but has no depth resolution. Therefore, most biomechanics studies using QPM today is confined to simple cells, such as RBCs, without internal organelles. An important instrument that will greatly extend the biomedical applications of QPM is to develop next generation microscope with 3D capability and sufficient temporal resolution to study biomechanics of complex eukaryotic cells including the mechanics of their internal compartments. For eukaryotic cells, the depth sectioning capability is critical and should be sufficient to distinguish nucleic membrane fluctuations from plasma membrane fluctuations. Further, this microscope must provide high temporal resolution since typical eukaryotes membranes are substantially stiffer than RBCs. A confocal reflectance quantitative phase microscope is presented based on multi-pinhole scanning, with the capabilities of higher temporal resolution and sensitivity for nucleic and plasma membranes of eukaryotic cells. System hardware is developed based on an array of confocal pinhole generated by using the `ON' state of subset of micro-mirrors of digital micro-mirror device (DMD, from Texas Instruments) and high-speed raster scanning provides 14ms imaging speed in wide-field mode. A common path interferometer is integrated at the imaging arm for detection of specimens' quantitative phase information. Theoretical investigation of quantitative phase reconstructed from system is investigated and application of system is presented for dimensional fluctuations measurements of both cellular plasma and nucleic membranes of embryonic stem cells.

Multiscale tomographic analysis of heterogeneous cast Al-Si-X alloys.

PubMed

Asghar, Z; Requena, G; Sket, F

2015-07-01

The three-dimensional microstructure of cast AlSi12Ni and AlSi10Cu5Ni2 alloys is investigated by laboratory X-ray computed tomography, synchrotron X-ray computed microtomography, light optical tomography and synchrotron X-ray computed microtomography with submicrometre resolution. The results obtained with each technique are correlated with the size of the scanned volumes and resolved microstructural features. Laboratory X-ray computed tomography is sufficient to resolve highly absorbing aluminides but eutectic and primary Si remain unrevealed. Synchrotron X-ray computed microtomography at ID15/ESRF gives better spatial resolution and reveals primary Si in addition to aluminides. Synchrotron X-ray computed microtomography at ID19/ESRF reveals all the phases ≥ ∼1 μm in volumes about 80 times smaller than laboratory X-ray computed tomography. The volumes investigated by light optical tomography and submicrometre synchrotron X-ray computed microtomography are much smaller than laboratory X-ray computed tomography but both techniques provide local chemical information on the types of aluminides. The complementary techniques applied enable a full three-dimensional characterization of the microstructure of the alloys at length scales ranging over six orders of magnitude. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

Propagation Diagnostic Simulations Using High-Resolution Equatorial Plasma Bubble Simulations

NASA Astrophysics Data System (ADS)

Rino, C. L.; Carrano, C. S.; Yokoyama, T.

2017-12-01

In a recent paper, under review, equatorial-plasma-bubble (EPB) simulations were used to conduct a comparative analysis of the EPB spectra characteristics with high-resolution in-situ measurements from the C/NOFS satellite. EPB realizations sampled in planes perpendicular to magnetic field lines provided well-defined EPB structure at altitudes penetrating both high and low-density regions. The average C/NOFS structure in highly disturbed regions showed nearly identical two-component inverse-power-law spectral characteristics as the measured EPB structure. This paper describes the results of PWE simulations using the same two-dimensional cross-field EPB realizations. New Irregularity Parameter Estimation (IPE) diagnostics, which are based on two-dimensional equivalent-phase-screen theory [A theory of scintillation for two-component power law irregularity spectra: Overview and numerical results, by Charles Carrano and Charles Rino, DOI: 10.1002/2015RS005903], have been successfully applied to extract two-component inverse-power-law parameters from measured intensity spectra. The EPB simulations [Low and Midlatitude Ionospheric Plasma DensityIrregularities and Their Effects on Geomagnetic Field, by Tatsuhiro Yokoyama and Claudia Stolle, DOI 10.1007/s11214-016-0295-7] have sufficient resolution to populate the structure scales (tens of km to hundreds of meters) that cause strong scintillation at GPS frequencies. The simulations provide an ideal geometry whereby the ramifications of varying structure along the propagation path can be investigated. It is well known path-integrated one-dimensional spectra increase the one-dimensional index by one. The relation requires decorrelation along the propagation path. Correlated structure would be interpreted as stochastic total-electron-content (TEC). The simulations are performed with unmodified structure. Because the EPB structure is confined to the central region of the sample planes, edge effects are minimized. Consequently, the propagated signal phase can be comparted to path-integrated phase for evaluating TEC extraction. Only the frequency dependence of phase scintillation distinguishes phase scintillation. The simulations allow scale-dependent exploration of remote-sensing diagnostics.

Experimental criteria for the determination of fractal parameters of premixed turbulent flames

NASA Astrophysics Data System (ADS)

Shepherd, I. G.; Cheng, Robert K.; Talbot, L.

1992-10-01

The influence of spatial resolution, digitization noise, the number of records used for averaging, and the method of analysis on the determination of the fractal parameters of a high Damköhler number, methane/air, premixed, turbulent stagnation-point flame are investigated in this paper. The flow exit velocity was 5 m/s and the turbulent Reynolds number was 70 based on a integral scale of 3 mm and a turbulent intensity of 7%. The light source was a copper vapor laser which delivered 20 nsecs, 5 mJ pulses at 4 kHz and the tomographic cross-sections of the flame were recorded by a high speed movie camera. The spatial resolution of the images is 155 × 121 μm/pixel with a field of view of 50 × 65 mm. The stepping caliper technique for obtaining the fractal parameters is found to give the clearest indication of the cutoffs and the effects of noise. It is necessary to ensemble average the results from more than 25 statistically independent images to reduce sufficiently the scatter in the fractal parameters. The effects of reduced spatial resolution on fractal plots are estimated by artificial degradation of the resolution of the digitized flame boundaries. The effect of pixel resolution, an apparent increase in flame length below the inner scale rolloff, appears in the fractal plots when the measurent scale is less than approximately twice the pixel resolution. Although a clearer determination of fractal parameters is obtained by local averaging of the flame boundaries which removes digitization noise, at low spatial resolution this technique can reduce the fractal dimension. The degree of fractal isotropy of the flame surface can have a significant effect on the estimation of the flame surface area and hence burning rate from two-dimensional images. To estimate this isotropy a determination of the outer cutoff is required and three-dimensional measurements are probably also necessary.

Peripheral Vasculature: High-Temporal- and High-Spatial-Resolution Three-dimensional Contrast-enhanced MR Angiography1

PubMed Central

Haider, Clifton R.; Glockner, James F.; Stanson, Anthony W.; Riederer, Stephen J.

2009-01-01

Purpose: To prospectively evaluate the feasibility of performing high-spatial-resolution (1-mm isotropic) time-resolved three-dimensional (3D) contrast material–enhanced magnetic resonance (MR) angiography of the peripheral vasculature with Cartesian acquisition with projection-reconstruction–like sampling (CAPR) and eightfold accelerated two-dimensional (2D) sensitivity encoding (SENSE). Materials and Methods: All studies were approved by the institutional review board and were HIPAA compliant; written informed consent was obtained from all participants. There were 13 volunteers (mean age, 41.9; range, 27–53 years). The CAPR sequence was adapted to provide 1-mm isotropic spatial resolution and a 5-second frame time. Use of different receiver coil element sizes for those placed on the anterior-to-posterior versus left-to-right sides of the field of view reduced signal-to-noise ratio loss due to acceleration. Results from eight volunteers were rated independently by two radiologists according to prominence of artifact, arterial to venous separation, vessel sharpness, continuity of arterial signal intensity in major arteries (anterior and posterior tibial, peroneal), demarcation of origin of major arteries, and overall diagnostic image quality. MR angiographic results in two patients with peripheral vascular disease were compared with their results at computed tomographic angiography. Results: The sequence exhibited no image artifact adversely affecting diagnostic image quality. Temporal resolution was evaluated to be sufficient in all cases, even with known rapid arterial to venous transit. The vessels were graded to have excellent sharpness, continuity, and demarcation of the origins of the major arteries. Distal muscular branches and the communicating and perforating arteries were routinely seen. Excellent diagnostic quality rating was given for 15 (94%) of 16 evaluations. Conclusion: The feasibility of performing high-diagnostic-quality time-resolved 3D contrast-enhanced MR angiography of the peripheral vasculature by using CAPR and eightfold accelerated 2D SENSE has been demonstrated. © RSNA, 2009 Supplemental material: http://radiology.rsna.org/lookup/suppl/doi:10.1148/radiol.2533081744/-/DC1 PMID:19789238

Comprehensive two-dimensional gas chromatography with flame ionization and time-of-flight mass spectrometry detection: qualitative and quantitative analysis of West Australian sandalwood oil.

PubMed

Shellie, Robert; Marriott, Philip; Morrison, Paul

2004-09-01

The use of gas chromatography (GC)-mass spectrometry (MS), GC-time-of-flight MS (TOFMS), comprehensive two-dimensional GC (GCxGC)-flame ionization detection (FID), and GCxGC-TOFMS is discussed for the characterization of the eight important representative components, including Z-alpha-santalol, epi-alpha-bisabolol, Z-alpha-trans-bergamotol, epi-beta-santalol, Z-beta-santalol, E,E-farnesol, Z-nuciferol, and Z-lanceol, in the oil of west Australian sandalwood (Santalum spicatum). Single-column GC-MS lacks the resolving power to separate all of the listed components as pure peaks and allow precise analytical measurement of individual component abundances. With enhanced peak resolution capabilities in GCxGC, these components are sufficiently well resolved to be quantitated using flame ionization detection, following initial characterization of components by using GCxGC-TOFMS.

Molecular imaging needles: dual-modality optical coherence tomography and fluorescence imaging of labeled antibodies deep in tissue

PubMed Central

Scolaro, Loretta; Lorenser, Dirk; Madore, Wendy-Julie; Kirk, Rodney W.; Kramer, Anne S.; Yeoh, George C.; Godbout, Nicolas; Sampson, David D.; Boudoux, Caroline; McLaughlin, Robert A.

2015-01-01

Molecular imaging using optical techniques provides insight into disease at the cellular level. In this paper, we report on a novel dual-modality probe capable of performing molecular imaging by combining simultaneous three-dimensional optical coherence tomography (OCT) and two-dimensional fluorescence imaging in a hypodermic needle. The probe, referred to as a molecular imaging (MI) needle, may be inserted tens of millimeters into tissue. The MI needle utilizes double-clad fiber to carry both imaging modalities, and is interfaced to a 1310-nm OCT system and a fluorescence imaging subsystem using an asymmetrical double-clad fiber coupler customized to achieve high fluorescence collection efficiency. We present, to the best of our knowledge, the first dual-modality OCT and fluorescence needle probe with sufficient sensitivity to image fluorescently labeled antibodies. Such probes enable high-resolution molecular imaging deep within tissue. PMID:26137379

Sufficient Forecasting Using Factor Models

PubMed Central

Fan, Jianqing; Xue, Lingzhou; Yao, Jiawei

2017-01-01

We consider forecasting a single time series when there is a large number of predictors and a possible nonlinear effect. The dimensionality was first reduced via a high-dimensional (approximate) factor model implemented by the principal component analysis. Using the extracted factors, we develop a novel forecasting method called the sufficient forecasting, which provides a set of sufficient predictive indices, inferred from high-dimensional predictors, to deliver additional predictive power. The projected principal component analysis will be employed to enhance the accuracy of inferred factors when a semi-parametric (approximate) factor model is assumed. Our method is also applicable to cross-sectional sufficient regression using extracted factors. The connection between the sufficient forecasting and the deep learning architecture is explicitly stated. The sufficient forecasting correctly estimates projection indices of the underlying factors even in the presence of a nonparametric forecasting function. The proposed method extends the sufficient dimension reduction to high-dimensional regimes by condensing the cross-sectional information through factor models. We derive asymptotic properties for the estimate of the central subspace spanned by these projection directions as well as the estimates of the sufficient predictive indices. We further show that the natural method of running multiple regression of target on estimated factors yields a linear estimate that actually falls into this central subspace. Our method and theory allow the number of predictors to be larger than the number of observations. We finally demonstrate that the sufficient forecasting improves upon the linear forecasting in both simulation studies and an empirical study of forecasting macroeconomic variables. PMID:29731537

Aerodynamic force measurement on a large-scale model in a short duration test facility

NASA Astrophysics Data System (ADS)

Tanno, H.; Kodera, M.; Komuro, T.; Sato, K.; Takahasi, M.; Itoh, K.

2005-03-01

A force measurement technique has been developed for large-scale aerodynamic models with a short test time. The technique is based on direct acceleration measurements, with miniature accelerometers mounted on a test model suspended by wires. Measuring acceleration at two different locations, the technique can eliminate oscillations from natural vibration of the model. The technique was used for drag force measurements on a 3m long supersonic combustor model in the HIEST free-piston driven shock tunnel. A time resolution of 350μs is guaranteed during measurements, whose resolution is enough for ms order test time in HIEST. To evaluate measurement reliability and accuracy, measured values were compared with results from a three-dimensional Navier-Stokes numerical simulation. The difference between measured values and numerical simulation values was less than 5%. We conclude that this measurement technique is sufficiently reliable for measuring aerodynamic force within test durations of 1ms.

Three-dimensional shape analysis of miarolitic cavities and enclaves in the Kakkonda granite by X-ray computed tomography

NASA Astrophysics Data System (ADS)

Ohtani, Tomoyuki; Nakano, Tsukasa; Nakashima, Yoshito; Muraoka, Hirofumi

2001-11-01

Three-dimensional shape analysis of miarolitic cavities and enclaves from the Kakkonda granite, NE Japan, was performed by X-ray computed tomography (CT) and image analysis. The three-dimensional shape of the miarolitic cavities and enclaves was reconstructed by stacked two-dimensional CT slice images with an in-plane resolution of 0.3 mm and an inter-slice spacing of 1 mm. An ellipsoid was fitted to each reconstructed object by the image processing programs. The shortest, intermediate, and longest axes of the ellipsoids fitted to miarolitic cavities had E-W, N-S, and vertical directions, respectively. The shortest axes of the ellipsoids fitted to enclaves were sub-vertical to vertical. Three-dimensional strains calculated from miarolitic cavities and enclaves have E-W and vertical shortening, respectively. The shape characteristics of miarolitic cavities probably reflect regional stress during the late magmatic stage, and those of enclaves reflect shortening by later-intruded magma or body rotation during the early magmatic stage. The miarolitic cavities may not be strained homogeneously with the surrounding granite, because the competence of minerals is different from that of the fluid-filled cavities. Although the strain markers require sufficient contrast between their CT numbers and those of the surrounding minerals, this method has several advantages over conventional methods, including the fact that it is non-destructive, expedient, and allows direct three-dimensional observation of each object.

Observations and mechanisms of GATE waterspouts

NASA Technical Reports Server (NTRS)

Simpson, J.; Mccumber, M. C.; Morton, B. R.; Penc, R. S.

1986-01-01

The present numerical and observational investigation of interacting cumulus processes implicated in the formation of waterspouts, the GATE database for days 261 and 186 is noted to imply that the existence of cumulus-scale parent vortices is a necessary (albeit not sufficient) condition for the production of waterspouts. A high resolution version of the Schlessinger (1975) three-dimensional cumulus model with a Kessler (1969) type precipitation scheme is used to analyze cumulus-scale vorticity organization, which on the two days in question exhibited contrasting thermal stratification and cloud features. The observations from both days suggest that the waterspouts formed ahead of the wind shift, due to the passage of a gust front.

A Mach-Zender digital holographic microscope with sub-micrometer resolution for imaging and tracking of marine micro-organisms

NASA Astrophysics Data System (ADS)

Kühn, Jonas; Niraula, Bimochan; Liewer, Kurt; Kent Wallace, J.; Serabyn, Eugene; Graff, Emilio; Lindensmith, Christian; Nadeau, Jay L.

2014-12-01

Digital holographic microscopy is an ideal tool for investigation of microbial motility. However, most designs do not exhibit sufficient spatial resolution for imaging bacteria. In this study we present an off-axis Mach-Zehnder design of a holographic microscope with spatial resolution of better than 800 nm and the ability to resolve bacterial samples at varying densities over a 380 μm × 380 μm × 600 μm three-dimensional field of view. Larger organisms, such as protozoa, can be resolved in detail, including cilia and flagella. The instrument design and performance are presented, including images and tracks of bacterial and protozoal mixed samples and pure cultures of six selected species. Organisms as small as 1 μm (bacterial spores) and as large as 60 μm (Paramecium bursaria) may be resolved and tracked without changes in the instrument configuration. Finally, we present a dilution series investigating the maximum cell density that can be imaged, a type of analysis that has not been presented in previous holographic microscopy studies.

Electron tomography of HEK293T cells using scanning electron microscope-based scanning transmission electron microscopy.

PubMed

You, Yun-Wen; Chang, Hsun-Yun; Liao, Hua-Yang; Kao, Wei-Lun; Yen, Guo-Ji; Chang, Chi-Jen; Tsai, Meng-Hung; Shyue, Jing-Jong

2012-10-01

Based on a scanning electron microscope operated at 30 kV with a homemade specimen holder and a multiangle solid-state detector behind the sample, low-kV scanning transmission electron microscopy (STEM) is presented with subsequent electron tomography for three-dimensional (3D) volume structure. Because of the low acceleration voltage, the stronger electron-atom scattering leads to a stronger contrast in the resulting image than standard TEM, especially for light elements. Furthermore, the low-kV STEM yields less radiation damage to the specimen, hence the structure can be preserved. In this work, two-dimensional STEM images of a 1-μm-thick cell section with projection angles between ±50° were collected, and the 3D volume structure was reconstructed using the simultaneous iterative reconstructive technique algorithm with the TomoJ plugin for ImageJ, which are both public domain software. Furthermore, the cross-sectional structure was obtained with the Volume Viewer plugin in ImageJ. Although the tilting angle is constrained and limits the resulting structural resolution, slicing the reconstructed volume generated the depth profile of the thick specimen with sufficient resolution to examine cellular uptake of Au nanoparticles, and the final position of these nanoparticles inside the cell was imaged.

Three dimensional canonical singularity and five dimensional N = 1 SCFT

NASA Astrophysics Data System (ADS)

Xie, Dan; Yau, Shing-Tung

2017-06-01

We conjecture that every three dimensional canonical singularity defines a five dimensional N = 1 SCFT. Flavor symmetry can be found from singularity structure: non-abelian flavor symmetry is read from the singularity type over one dimensional singular locus. The dimension of Coulomb branch is given by the number of compact crepant divisors from a crepant resolution of singularity. The detailed structure of Coulomb branch is described as follows: a) a chamber of Coulomb branch is described by a crepant resolution, and this chamber is given by its Nef cone and the prepotential is computed from triple intersection numbers; b) Crepant resolution is not unique and different resolutions are related by flops; Nef cones from crepant resolutions form a fan which is claimed to be the full Coulomb branch.

The usefulness of three-dimensional imaging in the diagnosis and treatment of clinically ambiguous gingival swelling.

PubMed

Kabashima, Hiroaki; Mizobe, Kunitaka; Nakamuta, Hiroyoshi; Fujiwara, Hiroaki; Okamura, Kazutoshi; Unemori, Masako; Akamine, Akifumi; Yoshiura, Kazunori; Maeda, Katsumasa

2011-06-01

We evaluated and treated a 54-year-old woman with gingival swelling. Conventional intraoral and panoramic radiography did not provide sufficient information for either determining the cause of gingival swelling or planning treatment of clinical symptoms. The 3D Accuitomo XYZ Slice View Tomograph (3DX) is a compact dental computed tomography device that allowed for accurate identification and optimal treatment of the causes of gingival swelling. At four years after treatment, 3DX radiographs showed no abnormalities in treated teeth or healing of surrounding structures. We conclude that high-resolution 3D images obtained with 3DX promise to be very effective for diagnosing oral diseases and determining effective treatment.

Physics of Core-Collapse Supernovae in Three Dimensions: A Sneak Preview

NASA Astrophysics Data System (ADS)

Janka, Hans-Thomas; Melson, Tobias; Summa, Alexander

2016-10-01

Nonspherical mass motions are a generic feature of core-collapse supernovae, and hydrodynamic instabilities play a crucial role in the explosion mechanism. The first successful neutrino-driven explosions could be obtained with self-consistent, first-principles simulations in three spatial dimensions. But three-dimensional (3D) models tend to be less prone to explosion than the corresponding axisymmetric two-dimensional (2D) ones. The reason is that 3D turbulence leads to energy cascading from large to small spatial scales, the inverse of the 2D case, thus disfavoring the growth of buoyant plumes on the largest scales. Unless the inertia to explode simply reflects a lack of sufficient resolution in relevant regions, some important component of robust and sufficiently energetic neutrino-powered explosions may still be missing. Such a deficit could be associated with progenitor properties such as rotation, magnetic fields, or precollapse perturbations, or with microphysics that could cause enhancement of neutrino heating behind the shock. 3D simulations have also revealed new phenomena that are not present in 2D ones, such as spiral modes of the standing accretion shock instability (SASI) and a stunning dipolar lepton-number emission self-sustained asymmetry (LESA). Both impose time- and direction-dependent variations on the detectable neutrino signal. The understanding of these effects and of their consequences is still in its infancy.

Nonhydrostatic simulation of hyperpycnal river plumes on sloping continental shelves: Flow structures and nonhydrostatic effect

NASA Astrophysics Data System (ADS)

Tseng, Chien-Yung; Chou, Yi-Ju

2018-04-01

A three-dimensional nonhydrostatic coastal model SUNTANS is used to study hyperpycnal plumes on sloping continental shelves with idealized domain setup. The study aims to examine the nonhydrostatic effect of the plunging hyperpycnal plume and the associated flow structures on different shelf slopes. The unstructured triangular grid in SUNTANS allows for local refinement of the grid size for regions in which the flow varies abruptly, while retaining low-cost computation using the coarse grid resolution for regions in which the flow is more uniform. These nonhydrostatic simulations reveal detailed three-dimensional flow structures in both transient and steady states. Via comparison with the hydrostatic simulation, we show that the nonhydrostatic effect is particularly important before plunging, when the plume is subject to significant changes in both the along-shore and vertical directions. After plunging, where the plume becomes an undercurrent that is more spatially uniform, little difference is found between the hydrostatic and nonhydrostatic simulations in the present gentle- and mild-slope cases. A grid-dependence study shows that the nonhydrostatic effect can be seen only when the grid resolution is sufficiently fine that the calculation is not overly diffusive. A depth-integrated momentum budget analysis is then conducted to show that the flow convergence due to plunging is an important factor in the three-dimensional flow structures. Moreover, it shows that the nonhydrostatic effect becomes more important as the slope increases, and in the steep-slope case, neglect of transport of the vertical momentum during plunging in the hydrostatic case further leads to an erroneous prediction for the undercurrent.

Numerical solutions of the Navier-Stokes equations for the supersonic laminar flow over a two-dimensional compression corner

NASA Technical Reports Server (NTRS)

Carter, J. E.

1972-01-01

Numerical solutions have been obtained for the supersonic, laminar flow over a two-dimensional compression corner. These solutions were obtained as steady-state solutions to the unsteady Navier-Stokes equations using the finite difference method of Brailovskaya, which has second-order accuracy in the spatial coordinates. Good agreement was obtained between the computed results and wall pressure distributions measured experimentally for Mach numbers of 4 and 6.06, and respective Reynolds numbers, based on free-stream conditions and the distance from the leading edge to the corner. In those calculations, as well as in others, sufficient resolution was obtained to show the streamline pattern in the separation bubble. Upstream boundary conditions to the compression corner flow were provided by numerically solving the unsteady Navier-Stokes equations for the flat plate flow field, beginning at the leading edge. The compression corner flow field was enclosed by a computational boundary with the unknown boundary conditions supplied by extrapolation from internally computed points.

Biological implications of lab-on-a-chip devices fabricated using multi-jet modelling and stereolithography processes

NASA Astrophysics Data System (ADS)

Zhu, Feng; Macdonald, Niall; Skommer, Joanna; Wlodkowic, Donald

2015-06-01

Current microfabrication methods are often restricted to two-dimensional (2D) or two and a half dimensional (2.5D) structures. Those fabrication issues can be potentially addressed by emerging additive manufacturing technologies. Despite rapid growth of additive manufacturing technologies in tissue engineering, microfluidics has seen relatively little developments with regards to adopting 3D printing for rapid fabrication of complex chip-based devices. This has been due to two major factors: lack of sufficient resolution of current rapid-prototyping methods (usually >100 μm ) and optical transparency of polymers to allow in vitro imaging of specimens. We postulate that adopting innovative fabrication processes can provide effective solutions for prototyping and manufacturing of chip-based devices with high-aspect ratios (i.e. above ration of 20:1). This work provides a comprehensive investigation of commercially available additive manufacturing technologies as an alternative for rapid prototyping of complex monolithic Lab-on-a-Chip devices for biological applications. We explored both multi-jet modelling (MJM) and several stereolithography (SLA) processes with five different 3D printing resins. Compared with other rapid prototyping technologies such as PDMS soft lithography and infrared laser micromachining, we demonstrated that selected SLA technologies had superior resolution and feature quality. We also for the first time optimised the post-processing protocols and demonstrated polymer features under scanning electronic microscope (SEM). Finally we demonstrate that selected SLA polymers have optical properties enabling high-resolution biological imaging. A caution should be, however, exercised as more work is needed to develop fully bio-compatible and non-toxic polymer chemistries.

A double stain for total and oxidized proteins from two-dimensional fingerprints.

PubMed

Talent, J M; Kong, Y; Gracy, R W

1998-10-01

Oxidative modification of proteins plays a major role in the etiology of aging and age-related diseases. For example, in Alzheimer's disease, although evidence points to oxidation of proteins as a causative factor in loss of cognitive abilities, it is not known which specific proteins of the brain are most susceptible to these modifications. Thus, it is of interest to identify the specific proteins which are susceptible to oxidation in vivo. Two-dimensional protein fingerprint methods offer the analytical potential for resolution of thousands of individual proteins from tissues, and the oxidized proteins can be visualized with immunological probes. Sensitive methods permit recovery and sufficient amino acid sequencing to identify these proteins. However, for such analyses it is essential to simultaneously analyze both protein content and level of oxidation. We have evaluated several approaches, identified the sources of artifacts and interferences, and developed a double-staining procedure that allows visualization and quantitation of total protein patterns as well as the specific oxidized proteins from two-dimensional protein fingerprints. The method has been applied to cells grown in culture and to tissue extracts from young and old animals. Copyright 1998 Academic Press.

Novel eye-safe line scanning 3D laser-radar

NASA Astrophysics Data System (ADS)

Eberle, B.; Kern, Tobias; Hammer, Marcus; Schwanke, Ullrich; Nowak, Heinrich

2014-10-01

Today, the civil market provides quite a number of different 3D-Sensors covering ranges up to 1 km. Typically these sensors are based on single element detectors which suffer from the drawback of spatial resolution at larger distances. Tasks demanding reliable object classification at long ranges can be fulfilled only by sensors consisting of detector arrays. They ensure sufficient frame rates and high spatial resolution. Worldwide there are many efforts in developing 3D-detectors, based on two-dimensional arrays. This paper presents first results on the performance of a recently developed 3D imaging laser radar sensor, working in the short wave infrared (SWIR) at 1.5 μm. It consists of a novel Cadmium Mercury Telluride (CMT) linear array APD detector with 384x1 elements at a pitch of 25 μm, developed by AIM Infrarot Module GmbH. The APD elements are designed to work in the linear (non-Geiger) mode. Each pixel will provide the time of flight measurement, and, due to the linear detection mode, allowing the detection of three successive echoes. The resolution in depth is 15 cm, the maximum repetition rate is 4 kHz. We discuss various sensor concepts regarding possible applications and their dependence on system parameters like field of view, frame rate, spatial resolution and range of operation.

Numerical viscosity and resolution of high-order weighted essentially nonoscillatory schemes for compressible flows with high Reynolds numbers.

PubMed

Zhang, Yong-Tao; Shi, Jing; Shu, Chi-Wang; Zhou, Ye

2003-10-01

A quantitative study is carried out in this paper to investigate the size of numerical viscosities and the resolution power of high-order weighted essentially nonoscillatory (WENO) schemes for solving one- and two-dimensional Navier-Stokes equations for compressible gas dynamics with high Reynolds numbers. A one-dimensional shock tube problem, a one-dimensional example with parameters motivated by supernova and laser experiments, and a two-dimensional Rayleigh-Taylor instability problem are used as numerical test problems. For the two-dimensional Rayleigh-Taylor instability problem, or similar problems with small-scale structures, the details of the small structures are determined by the physical viscosity (therefore, the Reynolds number) in the Navier-Stokes equations. Thus, to obtain faithful resolution to these small-scale structures, the numerical viscosity inherent in the scheme must be small enough so that the physical viscosity dominates. A careful mesh refinement study is performed to capture the threshold mesh for full resolution, for specific Reynolds numbers, when WENO schemes of different orders of accuracy are used. It is demonstrated that high-order WENO schemes are more CPU time efficient to reach the same resolution, both for the one-dimensional and two-dimensional test problems.

[Depiction of the cranial nerves around the cavernous sinus by 3D reversed FISP with diffusion weighted imaging (3D PSIF-DWI)].

PubMed

Ishida, Go; Oishi, Makoto; Jinguji, Shinya; Yoneoka, Yuichiro; Sato, Mitsuya; Fujii, Yukihiko

2011-10-01

To evaluate the anatomy of cranial nerves running in and around the cavernous sinus, we employed three-dimensional reversed fast imaging with steady-state precession (FISP) with diffusion weighted imaging (3D PSIF-DWI) on 3-T magnetic resonance (MR) system. After determining the proper parameters to obtain sufficient resolution of 3D PSIF-DWI, we collected imaging data of 20-side cavernous regions in 10 normal subjects. 3D PSIF-DWI provided high contrast between the cranial nerves and other soft tissues, fluid, and blood in all subjects. We also created volume-rendered images of 3D PSIF-DWI and anatomically evaluated the reliability of visualizing optic, oculomotor, trochlear, trigeminal, and abducens nerves on 3D PSIF-DWI. All 20 sets of cranial nerves were visualized and 12 trochlear nerves and 6 abducens nerves were partially identified. We also presented preliminary clinical experiences in two cases with pituitary adenomas. The anatomical relationship between the tumor and cranial nerves running in and around the cavernous sinus could be three-dimensionally comprehended by 3D PSIF-DWI and the volume-rendered images. In conclusion, 3D PSIF-DWI has great potential to provide high resolution "cranial nerve imaging", which visualizes the whole length of the cranial nerves including the parts in the blood flow as in the cavernous sinus region.

Material Properties of Human Ocular Tissue at 7-µm Resolution.

PubMed

Rohrbach, Daniel; Ito, Kazuyo; Lloyd, Harriet O; Silverman, Ronald H; Yoshida, Kenji; Yamaguchi, Tadashi; Mamou, Jonathan

2017-09-01

Quantitative assessment of the material properties of ocular tissues can provide valuable information for investigating several ophthalmic diseases. Quantitative acoustic microscopy (QAM) offers a means of obtaining such information, but few QAM investigations have been conducted on human ocular tissue. We imaged the optic nerve (ON) and iridocorneal angle in 12-µm deparaffinized sections of the human eye using a custom-built acoustic microscope with a 250-MHz transducer (7-µm lateral resolution). The two-dimensional QAM maps of ultrasound attenuation (α), speed of sound ( c), acoustic impedance ( Z), bulk modulus ( K), and mass density (ρ) were generated. Scanned samples were then stained and imaged by light microscopy for comparison with QAM maps. The spatial resolution and contrast of scanning acoustic microscopy (SAM) maps were sufficient to resolve anatomic layers of the retina (Re); anatomic features in SAM maps corresponded to those seen by light microscopy. Significant variations of the acoustic parameters were found. For example, the sclera was 220 MPa stiffer than Re, choroid, and ON tissue. To the authors' knowledge, this is the first systematic study to assess c, Z, K, ρ, and α of human ocular tissue at the high ultrasound frequencies used in this study.

On the ejection-induced instability in Navier-Stokes solutions of unsteady separation.

PubMed

Obabko, Aleksandr V; Cassel, Kevin W

2005-05-15

Numerical solutions of the flow induced by a thick-core vortex have been obtained using the unsteady, two-dimensional Navier-Stokes equations. The presence of the vortex causes an adverse pressure gradient along the surface, which leads to unsteady separation. The calculations by Brinckman and Walker for a similar flow identify a possible instability, purported to be an inviscid Rayleigh instability, in the region where ejection of near-wall vorticity occurs during the unsteady separation process. In results for a range of Reynolds numbers in the present investigation, the oscillations are also found to occur. However, they can be eliminated with increased grid resolution. Despite this behaviour, the instability may be physical but requires a sufficient amplitude of disturbances to be realized.

Unsteady, one-dimensional gas dynamics computations using a TVD type sequential solver

NASA Technical Reports Server (NTRS)

Thakur, Siddharth; Shyy, Wei

1992-01-01

The efficacy of high resolution convection schemes to resolve sharp gradient in unsteady, 1D flows is examined using the TVD concept based on a sequential solution algorithm. Two unsteady flow problems are considered which include the problem involving the interaction of the various waves in a shock tube with closed reflecting ends and the problem involving the unsteady gas dynamics in a tube with closed ends subject to an initial pressure perturbation. It is concluded that high accuracy convection schemes in a sequential solution framework are capable of resolving discontinuities in unsteady flows involving complex gas dynamics. However, a sufficient amount of dissipation is required to suppress oscillations near discontinuities in the sequential approach, which leads to smearing of the solution profiles.

Three-dimensional reconstruction and display of the heart, lungs and circulation by multiplanar X-ray scanning videodensitometry

NASA Technical Reports Server (NTRS)

Robb, R. A.; Ritman, E. L.; Wood, E. H.

1975-01-01

A device was developed which makes possible the dynamic reconstruction of the heart and lungs within the intact thorax of a living dog or human and which can record approximately 30 multiplanar X-ray images of the thorax practically instantaneously, and at frequent enough intervals of time and with sufficient density and spatial resolution to capture and resolve the most rapid changes in cardiac structural detail throughout each cardiac cycle. It can be installed in a clinical diagnostic setting as well as in a research environment and its construction and application for determination and display in real-time modes of cross sections of the functioning thorax and its contents of living animals and man is technologically feasible.

Interactomes to Biological Phase Space: a call to begin thinking at a new level in computational biology.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Davidson, George S.; Brown, William Michael

2007-09-01

Techniques for high throughput determinations of interactomes, together with high resolution protein collocalizations maps within organelles and through membranes will soon create a vast resource. With these data, biological descriptions, akin to the high dimensional phase spaces familiar to physicists, will become possible. These descriptions will capture sufficient information to make possible realistic, system-level models of cells. The descriptions and the computational models they enable will require powerful computing techniques. This report is offered as a call to the computational biology community to begin thinking at this scale and as a challenge to develop the required algorithms and codes tomore » make use of the new data.3« less

Benchmarking the mesoscale variability in global ocean eddy-permitting numerical systems

NASA Astrophysics Data System (ADS)

Cipollone, Andrea; Masina, Simona; Storto, Andrea; Iovino, Doroteaciro

2017-10-01

The role of data assimilation procedures on representing ocean mesoscale variability is assessed by applying eddy statistics to a state-of-the-art global ocean reanalysis (C-GLORS), a free global ocean simulation (performed with the NEMO system) and an observation-based dataset (ARMOR3D) used as an independent benchmark. Numerical results are computed on a 1/4 ∘ horizontal grid (ORCA025) and share the same resolution with ARMOR3D dataset. This "eddy-permitting" resolution is sufficient to allow ocean eddies to form. Further to assessing the eddy statistics from three different datasets, a global three-dimensional eddy detection system is implemented in order to bypass the need of regional-dependent definition of thresholds, typical of commonly adopted eddy detection algorithms. It thus provides full three-dimensional eddy statistics segmenting vertical profiles from local rotational velocities. This criterion is crucial for discerning real eddies from transient surface noise that inevitably affects any two-dimensional algorithm. Data assimilation enhances and corrects mesoscale variability on a wide range of features that cannot be well reproduced otherwise. The free simulation fairly reproduces eddies emerging from western boundary currents and deep baroclinic instabilities, while underestimates shallower vortexes that populate the full basin. The ocean reanalysis recovers most of the missing turbulence, shown by satellite products , that is not generated by the model itself and consistently projects surface variability deep into the water column. The comparison with the statistically reconstructed vertical profiles from ARMOR3D show that ocean data assimilation is able to embed variability into the model dynamics, constraining eddies with in situ and altimetry observation and generating them consistently with local environment.

Multiscale solute transport upscaling for a three-dimensional hierarchical porous medium

NASA Astrophysics Data System (ADS)

Zhang, Mingkan; Zhang, Ye

2015-03-01

A laboratory-generated hierarchical, fully heterogeneous aquifer model (FHM) provides a reference for developing and testing an upscaling approach that integrates large-scale connectivity mapping with flow and transport modeling. Based on the FHM, three hydrostratigraphic models (HSMs) that capture lithological (static) connectivity at different resolutions are created, each corresponding to a sedimentary hierarchy. Under increasing system lnK variances (0.1, 1.0, 4.5), flow upscaling is first conducted to calculate equivalent hydraulic conductivity for individual connectivity (or unit) of the HSMs. Given the computed flow fields, an instantaneous, conservative tracer test is simulated by all models. For the HSMs, two upscaling formulations are tested based on the advection-dispersion equation (ADE), implementing space versus time-dependent macrodispersivity. Comparing flow and transport predictions of the HSMs against those of the reference model, HSMs capturing connectivity at increasing resolutions are more accurate, although upscaling errors increase with system variance. Results suggest: (1) by explicitly modeling connectivity, an enhanced degree of freedom in representing dispersion can improve the ADE-based upscaled models by capturing non-Fickian transport of the FHM; (2) when connectivity is sufficiently resolved, the type of data conditioning used to model transport becomes less critical. Data conditioning, however, is influenced by the prediction goal; (3) when aquifer is weakly-to-moderately heterogeneous, the upscaled models adequately capture the transport simulation of the FHM, despite the existence of hierarchical heterogeneity at smaller scales. When aquifer is strongly heterogeneous, the upscaled models become less accurate because lithological connectivity cannot adequately capture preferential flows; (4) three-dimensional transport connectivities of the hierarchical aquifer differ quantitatively from those analyzed for two-dimensional systems. This article was corrected on 7 MAY 2015. See the end of the full text for details.

Three Dimensional High-Resolution Reconstruction of the Ionosphere Over the Very Large Array

DTIC Science & Technology

2010-12-15

Watts Progress Report, Dec 10; 1 Final Report: Three Dimensional High-Resolution Reconstruction of the Ionosphere over the Very Large Array...proposed research is reconstruct the three-dimensional regional electron density profile of Earth’s ionosphere with spatial resolution of better than 10 km...10x better sensitivity to total electron content (TEC, or chord integrated density) in the ionosphere that does GPS. The proposal funds the

Isotropic-resolution linear-array-based photoacoustic computed tomography through inverse Radon transform

NASA Astrophysics Data System (ADS)

Li, Guo; Xia, Jun; Li, Lei; Wang, Lidai; Wang, Lihong V.

2015-03-01

Linear transducer arrays are readily available for ultrasonic detection in photoacoustic computed tomography. They offer low cost, hand-held convenience, and conventional ultrasonic imaging. However, the elevational resolution of linear transducer arrays, which is usually determined by the weak focus of the cylindrical acoustic lens, is about one order of magnitude worse than the in-plane axial and lateral spatial resolutions. Therefore, conventional linear scanning along the elevational direction cannot provide high-quality three-dimensional photoacoustic images due to the anisotropic spatial resolutions. Here we propose an innovative method to achieve isotropic resolutions for three-dimensional photoacoustic images through combined linear and rotational scanning. In each scan step, we first elevationally scan the linear transducer array, and then rotate the linear transducer array along its center in small steps, and scan again until 180 degrees have been covered. To reconstruct isotropic three-dimensional images from the multiple-directional scanning dataset, we use the standard inverse Radon transform originating from X-ray CT. We acquired a three-dimensional microsphere phantom image through the inverse Radon transform method and compared it with a single-elevational-scan three-dimensional image. The comparison shows that our method improves the elevational resolution by up to one order of magnitude, approaching the in-plane lateral-direction resolution. In vivo rat images were also acquired.

Determination of minority-carrier lifetime and surface recombination velocity with high spacial resolution

NASA Technical Reports Server (NTRS)

Watanabe, M.; Actor, G.; Gatos, H. C.

1977-01-01

Quantitative analysis of the electron beam induced current in conjunction with high-resolution scanning makes it possible to evaluate the minority-carrier lifetime three dimensionally in the bulk and the surface recombination velocity two dimensionally, with a high spacial resolution. The analysis is based on the concept of the effective excitation strength of the carriers which takes into consideration all possible recombination sources. Two-dimensional mapping of the surface recombination velocity of phosphorus-diffused silicon diodes is presented as well as a three-dimensional mapping of the changes in the minority-carrier lifetime in ion-implanted silicon.

Atomic-Resolution Spectrum Imaging of Semiconductor Nanowires.

PubMed

Zamani, Reza R; Hage, Fredrik S; Lehmann, Sebastian; Ramasse, Quentin M; Dick, Kimberly A

2018-03-14

Over the past decade, III-V heterostructure nanowires have attracted a surge of attention for their application in novel semiconductor devices such as tunneling field-effect transistors (TFETs). The functionality of such devices critically depends on the specific atomic arrangement at the semiconductor heterointerfaces. However, most of the currently available characterization techniques lack sufficient spatial resolution to provide local information on the atomic structure and composition of these interfaces. Atomic-resolution spectrum imaging by means of electron energy-loss spectroscopy (EELS) in the scanning transmission electron microscope (STEM) is a powerful technique with the potential to resolve structure and chemical composition with sub-angstrom spatial resolution and to provide localized information about the physical properties of the material at the atomic scale. Here, we demonstrate the use of atomic-resolution EELS to understand the interface atomic arrangement in three-dimensional heterostructures in semiconductor nanowires. We observed that the radial interfaces of GaSb-InAs heterostructure nanowires are atomically abrupt, while the axial interface in contrast consists of an interfacial region where intermixing of the two compounds occurs over an extended spatial region. The local atomic configuration affects the band alignment at the interface and, hence, the charge transport properties of devices such as GaSb-InAs nanowire TFETs. STEM-EELS thus represents a very promising technique for understanding nanowire physical properties, such as differing electrical behavior across the radial and axial heterointerfaces of GaSb-InAs nanowires for TFET applications.

Three-dimensional inversion recovery manganese-enhanced MRI of mouse brain using super-resolution reconstruction to visualize nuclei involved in higher brain function.

PubMed

Poole, Dana S; Plenge, Esben; Poot, Dirk H J; Lakke, Egbert A J F; Niessen, Wiro J; Meijering, Erik; van der Weerd, Louise

2014-07-01

The visualization of activity in mouse brain using inversion recovery spin echo (IR-SE) manganese-enhanced MRI (MEMRI) provides unique contrast, but suffers from poor resolution in the slice-encoding direction. Super-resolution reconstruction (SRR) is a resolution-enhancing post-processing technique in which multiple low-resolution slice stacks are combined into a single volume of high isotropic resolution using computational methods. In this study, we investigated, first, whether SRR can improve the three-dimensional resolution of IR-SE MEMRI in the slice selection direction, whilst maintaining or improving the contrast-to-noise ratio of the two-dimensional slice stacks. Second, the contrast-to-noise ratio of SRR IR-SE MEMRI was compared with a conventional three-dimensional gradient echo (GE) acquisition. Quantitative experiments were performed on a phantom containing compartments of various manganese concentrations. The results showed that, with comparable scan times, the signal-to-noise ratio of three-dimensional GE acquisition is higher than that of SRR IR-SE MEMRI. However, the contrast-to-noise ratio between different compartments can be superior with SRR IR-SE MEMRI, depending on the chosen inversion time. In vivo experiments were performed in mice receiving manganese using an implanted osmotic pump. The results showed that SRR works well as a resolution-enhancing technique in IR-SE MEMRI experiments. In addition, the SRR image also shows a number of brain structures that are more clearly discernible from the surrounding tissues than in three-dimensional GE acquisition, including a number of nuclei with specific higher brain functions, such as memory, stress, anxiety and reward behavior. Copyright © 2014 John Wiley & Sons, Ltd.

A high-resolution 3D ultrasonic system for rapid evaluation of the anterior and posterior segment.

PubMed

Peyman, Gholam A; Ingram, Charles P; Montilla, Leonardo G; Witte, Russell S

2012-01-01

Traditional ultrasound imaging systems for ophthalmology employ slow, mechanical scanning of a single-element ultrasound transducer. The goal was to demonstrate rapid examination of the anterior and posterior segment with a three-dimensional (3D) commercial ultrasound system incorporating high-resolution linear probe arrays. The 3D images of the porcine eye were generated in approximately 10 seconds by scanning one of two commercial linear arrays (25- and 50-MHz). Healthy enucleated pig eyes were compared with those with induced injury or placement of a foreign material (eg, metal). Rapid, volumetric imaging was also demonstrated in one human eye in vivo. The 50-MHz probe provided exquisite volumetric images of the anterior segment at a depth up to 15 mm and axial resolution of 30 μm. The 25-MHz probe provided a larger field of view (lateral X depth: 20 × 30 mm), sufficient for capturing the entire anterior and posterior segments of the pig eye, at a resolution of 60 μm. A 50-MHz scan through the human eyelid illustrated detailed structures of the Meibomian glands, cilia, cornea, and anterior segment back to the posterior capsule. The 3D system with its high-frequency ultrasound arrays, fast data acquisition, and volume rendering capability shows promise for investigating anterior and posterior structures of the eye. Copyright 2012, SLACK Incorporated.

Characterization of synthetic dyes by comprehensive two-dimensional liquid chromatography combining ion-exchange chromatography and fast ion-pair reversed-phase chromatography.

PubMed

Pirok, Bob W J; Knip, Jitske; van Bommel, Maarten R; Schoenmakers, Peter J

2016-03-04

In the late 19th century, newly invented synthetic dyes rapidly replaced the natural dyes on the market. The characterization of mixtures of these so-called early synthetic dyes is complicated through the occurrence of many impurities and degradation products. Conventional one-dimensional liquid chromatography does not suffice to obtain fingerprints with sufficient resolution and baseline integrity. Comprehensive two-dimensional liquid chromatography (LC×LC) is employed in this study, with ion-exchange chromatography in the first dimension and fast ion-pair liquid chromatography in the second. Retention in the first dimension is largely determined by the number of charges, while the selection of a small ion-pair reagent (tetramethylammonium hydroxide) in the second dimension causes retention to be largely determined by the molecular structure of the dye. As a result, there is a high degree of orthogonality of the two dimensions, similar to the values typically encountered in GC×GC. The proposed LC×LC method shows a theroretical peak capacity of about 2000 in an analysis time of about three hours. Clear, informative fingerprints are obtained that open a way to a more efficient characterization of dyes used in objects of cultural heritage. Copyright © 2016 Elsevier B.V. All rights reserved.

Gravitational lensing by a smoothly variable three-dimensional mass distribution

NASA Technical Reports Server (NTRS)

Lee, Man Hoi; Paczynski, Bohdan

1990-01-01

A smooth three-dimensional mass distribution is approximated by a model with multiple thin screens, with surface mass density varying smoothly on each screen. It is found that 16 screens are sufficient for a good approximation of the three-dimensional distribution of matter. It is also found that in this multiscreen model the distribution of amplifications of single images is dominated by the convergence due to matter within the beam. The shear caused by matter outside the beam has no significant effect. This finding considerably simplifies the modeling of lensing by a smooth three-dimensional mass distribution by effectively reducing the problem to one dimension, as it is sufficient to know the mass distribution along a straight light ray.

Imaging pathways in fractured rock using three-dimensional electrical resistivity tomography

USGS Publications Warehouse

Robinson, Judith; Slater, Lee; Johnson, Timothy B.; Shapiro, Allen M.; Tiedeman, Claire; Ntlargiannis, Dimitrios; Johnson, Carole D.; Day-Lewis, Frederick D.; Lacombe, Pierre; Imbrigiotta, Thomas; Lane, John W.

2016-01-01

Major challenges exist in delineating bedrock fracture zones because these cause abrupt changes in geological and hydrogeological properties over small distances. Borehole observations cannot sufficiently capture heterogeneity in these systems. Geophysical techniques offer the potential to image properties and processes in between boreholes. We used three-dimensional cross borehole electrical resistivity tomography (ERT) in a 9 m (diameter) × 15 m well field to capture high-resolution flow and transport processes in a fractured mudstone contaminated by chlorinated solvents, primarily trichloroethylene. Conductive (sodium bromide) and resistive (deionized water) injections were monitored in seven boreholes. Electrode arrays with isolation packers and fluid sampling ports were designed to enable acquisition of ERT measurements during pulsed tracer injections. Fracture zone locations and hydraulic pathways inferred from hydraulic head drawdown data were compared with electrical conductivity distributions from ERT measurements. Static ERT imaging has limited resolution to decipher individual fractures; however, these images showed alternating conductive and resistive zones, consistent with alternating laminated and massive mudstone units at the site. Tracer evolution and migration was clearly revealed in time-lapse ERT images and supported by in situ borehole vertical apparent conductivity profiles collected during the pulsed tracer test. While water samples provided important local information at the extraction borehole, ERT delineated tracer migration over spatial scales capturing the primary hydrogeological heterogeneity controlling flow and transport. The fate of these tracer injections at this scale could not have been quantified using borehole logging and/or borehole sampling methods alone.

Tip Vortex and Wake Characteristics of a Counterrotating Open Rotor

NASA Technical Reports Server (NTRS)

VanZante, Dale E.; Wernet, Mark P.

2012-01-01

One of the primary noise sources for Open Rotor systems is the interaction of the forward rotor tip vortex and blade wake with the aft rotor. NASA has collaborated with General Electric on the testing of a new generation of low noise, counterrotating Open Rotor systems. Three-dimensional particle image velocimetry measurements were acquired in the intra-rotor gap of the Historical Baseline blade set. The velocity measurements are of sufficient resolution to characterize the tip vortex size and trajectory as well as the rotor wake decay and turbulence character. The tip clearance vortex trajectory is compared to results from previously developed models. Forward rotor wake velocity profiles are shown. Results are presented in a form as to assist numerical modeling of Open Rotor system aerodynamics and acoustics.

Numerical Issues for Circulation Control Calculations

NASA Technical Reports Server (NTRS)

Swanson, Roy C., Jr.; Rumsey, Christopher L.

2006-01-01

Steady-state and time-accurate two-dimensional solutions of the compressible Reynolds-averaged Navier- Stokes equations are obtained for flow over the Lockheed circulation control (CC) airfoil and the General Aviation CC (GACC) airfoil. Numerical issues in computing circulation control flows such as the effects of grid resolution, boundary and initial conditions, and unsteadiness are addressed. For the Lockheed CC airfoil computed solutions are compared with detailed experimental data, which include velocity and Reynolds stress profiles. Three turbulence models, having either one or two transport equations, are considered. Solutions are obtained on a sequence of meshes, with mesh refinement primarily concentrated on the airfoil circular trailing edge. Several effects related to mesh refinement are identified. For example, sometimes sufficient mesh resolution can exclude nonphysical solutions, which can occur in CC airfoil calculations. Also, sensitivities of the turbulence models with mesh refinement are discussed. In the case of the GACC airfoil the focus is on the difference between steady-state and time-accurate solutions. A specific objective is to determine if there is self-excited vortex shedding from the jet slot lip.

3-D Ultrastructure of O. tauri: Electron Cryotomography of an Entire Eukaryotic Cell

PubMed Central

Henderson, Gregory P.; Gan, Lu; Jensen, Grant J.

2007-01-01

The hallmark of eukaryotic cells is their segregation of key biological functions into discrete, membrane-bound organelles. Creating accurate models of their ultrastructural complexity has been difficult in part because of the limited resolution of light microscopy and the artifact-prone nature of conventional electron microscopy. Here we explored the potential of the emerging technology electron cryotomography to produce three-dimensional images of an entire eukaryotic cell in a near-native state. Ostreococcus tauri was chosen as the specimen because as a unicellular picoplankton with just one copy of each organelle, it is the smallest known eukaryote and was therefore likely to yield the highest resolution images. Whole cells were imaged at various stages of the cell cycle, yielding 3-D reconstructions of complete chloroplasts, mitochondria, endoplasmic reticula, Golgi bodies, peroxisomes, microtubules, and putative ribosome distributions in-situ. Surprisingly, the nucleus was seen to open long before mitosis, and while one microtubule (or two in some predivisional cells) was consistently present, no mitotic spindle was ever observed, prompting speculation that a single microtubule might be sufficient to segregate multiple chromosomes. PMID:17710148

7 Å resolution in protein two-dimensional-crystal X-ray diffraction at Linac Coherent Light Source

PubMed Central

Pedrini, Bill; Tsai, Ching-Ju; Capitani, Guido; Padeste, Celestino; Hunter, Mark S.; Zatsepin, Nadia A.; Barty, Anton; Benner, W. Henry; Boutet, Sébastien; Feld, Geoffrey K.; Hau-Riege, Stefan P.; Kirian, Richard A.; Kupitz, Christopher; Messerschmitt, Marc; Ogren, John I.; Pardini, Tommaso; Segelke, Brent; Williams, Garth J.; Spence, John C. H.; Abela, Rafael; Coleman, Matthew; Evans, James E.; Schertler, Gebhard F. X.; Frank, Matthias; Li, Xiao-Dan

2014-01-01

Membrane proteins arranged as two-dimensional crystals in the lipid environment provide close-to-physiological structural information, which is essential for understanding the molecular mechanisms of protein function. Previously, X-ray diffraction from individual two-dimensional crystals did not represent a suitable investigational tool because of radiation damage. The recent availability of ultrashort pulses from X-ray free-electron lasers (XFELs) has now provided a means to outrun the damage. Here, we report on measurements performed at the Linac Coherent Light Source XFEL on bacteriorhodopsin two-dimensional crystals mounted on a solid support and kept at room temperature. By merging data from about a dozen single crystal diffraction images, we unambiguously identified the diffraction peaks to a resolution of 7 Å, thus improving the observable resolution with respect to that achievable from a single pattern alone. This indicates that a larger dataset will allow for reliable quantification of peak intensities, and in turn a corresponding increase in the resolution. The presented results pave the way for further XFEL studies on two-dimensional crystals, which may include pump–probe experiments at subpicosecond time resolution. PMID:24914166

Serial sectioning for examination of photoreceptor cell architecture by focused ion beam technology

PubMed Central

Mustafi, Debarshi; Avishai, Amir; Avishai, Nanthawan; Engel, Andreas; Heuer, Arthur; Palczewski, Krzysztof

2011-01-01

Structurally deciphering complex neural networks requires technology with sufficient resolution to allow visualization of single cells and their intimate surrounding connections. Scanning electron microscopy (SEM), coupled with serial ion ablation (SIA) technology, presents a new avenue to study these networks. SIA allows ion ablation to remove nanometer sections of tissue for SEM imaging, resulting in serial section data collection for three-dimensional reconstruction. Here we highlight a method for preparing retinal tissues for imaging of photoreceptors by SIA-SEM technology. We show that this technique can be used to visualize whole rod photoreceptors and the internal disc elements from wild-type (wt) mice. The distance parameters of the discs and photoreceptors are in good agreement with previous work with other methods. Moreover, we show that large planes of retinal tissue can be imaged at high resolution to display the packing of normal rods. Finally, SIA-SEM imaging of retinal tissue from a mouse model (Nrl−/−) with phenotypic changes akin to the human disease enhanced S-cone syndrome (ESCS) revealed a structural profile of overall photoreceptor ultrastructure and internal elements that accompany this disease. Overall, this work presents a new method to study photoreceptor cells at high structural resolution that has a broad applicability to the visual neuroscience field. PMID:21439323

An advanced stochastic weather generator for simulating 2-D high-resolution climate variables

NASA Astrophysics Data System (ADS)

Peleg, Nadav; Fatichi, Simone; Paschalis, Athanasios; Molnar, Peter; Burlando, Paolo

2017-07-01

A new stochastic weather generator, Advanced WEather GENerator for a two-dimensional grid (AWE-GEN-2d) is presented. The model combines physical and stochastic approaches to simulate key meteorological variables at high spatial and temporal resolution: 2 km × 2 km and 5 min for precipitation and cloud cover and 100 m × 100 m and 1 h for near-surface air temperature, solar radiation, vapor pressure, atmospheric pressure, and near-surface wind. The model requires spatially distributed data for the calibration process, which can nowadays be obtained by remote sensing devices (weather radar and satellites), reanalysis data sets and ground stations. AWE-GEN-2d is parsimonious in terms of computational demand and therefore is particularly suitable for studies where exploring internal climatic variability at multiple spatial and temporal scales is fundamental. Applications of the model include models of environmental systems, such as hydrological and geomorphological models, where high-resolution spatial and temporal meteorological forcing is crucial. The weather generator was calibrated and validated for the Engelberg region, an area with complex topography in the Swiss Alps. Model test shows that the climate variables are generated by AWE-GEN-2d with a level of accuracy that is sufficient for many practical applications.

Laboratory-based x-ray phase-contrast tomography enables 3D virtual histology

NASA Astrophysics Data System (ADS)

Töpperwien, Mareike; Krenkel, Martin; Quade, Felix; Salditt, Tim

2016-09-01

Due to the large penetration depth and small wavelength hard x-rays offer a unique potential for 3D biomedical and biological imaging, combining capabilities of high resolution and large sample volume. However, in classical absorption-based computed tomography, soft tissue only shows a weak contrast, limiting the actual resolution. With the advent of phase-contrast methods, the much stronger phase shift induced by the sample can now be exploited. For high resolution, free space propagation behind the sample is particularly well suited to make the phase shift visible. Contrast formation is based on the self-interference of the transmitted beam, resulting in object-induced intensity modulations in the detector plane. As this method requires a sufficiently high degree of spatial coherence, it was since long perceived as a synchrotron-based imaging technique. In this contribution we show that by combination of high brightness liquid-metal jet microfocus sources and suitable sample preparation techniques, as well as optimized geometry, detection and phase retrieval, excellent three-dimensional image quality can be obtained, revealing the anatomy of a cobweb spider in high detail. This opens up new opportunities for 3D virtual histology of small organisms. Importantly, the image quality is finally augmented to a level accessible to automatic 3D segmentation.

High-resolution ab initio three-dimensional x-ray diffraction microscopy

DOE PAGES

Chapman, Henry N.; Barty, Anton; Marchesini, Stefano; ...

2006-01-01

Coherent x-ray diffraction microscopy is a method of imaging nonperiodic isolated objects at resolutions limited, in principle, by only the wavelength and largest scattering angles recorded. We demonstrate x-ray diffraction imaging with high resolution in all three dimensions, as determined by a quantitative analysis of the reconstructed volume images. These images are retrieved from the three-dimensional diffraction data using no a priori knowledge about the shape or composition of the object, which has never before been demonstrated on a nonperiodic object. We also construct two-dimensional images of thick objects with greatly increased depth of focus (without loss of transverse spatialmore » resolution). These methods can be used to image biological and materials science samples at high resolution with x-ray undulator radiation and establishes the techniques to be used in atomic-resolution ultrafast imaging at x-ray free-electron laser sources.« less

Three-Dimensional Super-Resolution: Theory, Modeling, and Field Tests Results

NASA Technical Reports Server (NTRS)

Bulyshev, Alexander; Amzajerdian, Farzin; Roback, Vincent E.; Hines, Glenn; Pierrottet, Diego; Reisse, Robert

2014-01-01

Many flash lidar applications continue to demand higher three-dimensional image resolution beyond the current state-of-the-art technology of the detector arrays and their associated readout circuits. Even with the available number of focal plane pixels, the required number of photons for illuminating all the pixels may impose impractical requirements on the laser pulse energy or the receiver aperture size. Therefore, image resolution enhancement by means of a super-resolution algorithm in near real time presents a very attractive solution for a wide range of flash lidar applications. This paper describes a superresolution technique and illustrates its performance and merits for generating three-dimensional image frames at a video rate.

A Multi-Resolution Nonlinear Mapping Technique for Design and Analysis Applications

NASA Technical Reports Server (NTRS)

Phan, Minh Q.

1998-01-01

This report describes a nonlinear mapping technique where the unknown static or dynamic system is approximated by a sum of dimensionally increasing functions (one-dimensional curves, two-dimensional surfaces, etc.). These lower dimensional functions are synthesized from a set of multi-resolution basis functions, where the resolutions specify the level of details at which the nonlinear system is approximated. The basis functions also cause the parameter estimation step to become linear. This feature is taken advantage of to derive a systematic procedure to determine and eliminate basis functions that are less significant for the particular system under identification. The number of unknown parameters that must be estimated is thus reduced and compact models obtained. The lower dimensional functions (identified curves and surfaces) permit a kind of "visualization" into the complexity of the nonlinearity itself.

A Multi-Resolution Nonlinear Mapping Technique for Design and Analysis Application

NASA Technical Reports Server (NTRS)

Phan, Minh Q.

1997-01-01

This report describes a nonlinear mapping technique where the unknown static or dynamic system is approximated by a sum of dimensionally increasing functions (one-dimensional curves, two-dimensional surfaces, etc.). These lower dimensional functions are synthesized from a set of multi-resolution basis functions, where the resolutions specify the level of details at which the nonlinear system is approximated. The basis functions also cause the parameter estimation step to become linear. This feature is taken advantage of to derive a systematic procedure to determine and eliminate basis functions that are less significant for the particular system under identification. The number of unknown parameters that must be estimated is thus reduced and compact models obtained. The lower dimensional functions (identified curves and surfaces) permit a kind of "visualization" into the complexity of the nonlinearity itself.

Low--resolution vision in a velvet worm (Onychophora).

PubMed

Kirwan, John D; Graf, Josefine; Smolka, Jochen; Mayer, Georg; Henze, Miriam J; Nilsson, Dan-Eric

2018-06-04

Onychophorans, also known as velvet worms, possess a pair of simple lateral eyes, and are a key lineage with regard to the evolution of vision. They resemble ancient Cambrian forms, and are closely related to arthropods, which boast an unrivalled diversity of eye designs. Nonetheless, the visual capabilities of onychophorans have not been well explored. Here, we assessed the spatial resolution of the onychophoran Euperipatoides rowelli using behavioural experiments, three-dimensional reconstruction, anatomical and optical examinations, and modelling. Exploiting their spontaneous attraction towards dark objects, we found that E. rowelli can resolve stimuli that have the same average luminance as the background. Depending on the assumed contrast sensitivity of the animals, we estimate the spatial resolution to be in the range 15-40 deg. This results from an arrangement where the cornea and lens project the image largely behind the retina. The peculiar ellipsoid shape of the eye in combination with the asymmetric position and tilted orientation of the lens may improve spatial resolution in the forward direction. Nonetheless, the unordered network of interdigitating photoreceptors, which fills the whole eye chamber, precludes high-acuity vision. Our findings suggest that adult specimens of E. rowelli cannot spot or visually identify prey or conspecifics beyond a few centimetres from the eye, but the coarse spatial resolution that the animals exhibited in our experiments is likely to be sufficient to find shelter and suitable microhabitats from further away. To our knowledge, this is the first evidence of resolving vision in an onychophoran. © 2018. Published by The Company of Biologists Ltd.

Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy

NASA Astrophysics Data System (ADS)

Staier, Florian; Eipel, Heinz; Matula, Petr; Evsikov, Alexei V.; Kozubek, Michal; Cremer, Christoph; Hausmann, Michael

2011-09-01

With the development of novel fluorescence techniques, high resolution light microscopy has become a challenging technique for investigations of the three-dimensional (3D) micro-cosmos in cells and sub-cellular components. So far, all fluorescence microscopes applied for 3D imaging in biosciences show a spatially anisotropic point spread function resulting in an anisotropic optical resolution or point localization precision. To overcome this shortcoming, micro axial tomography was suggested which allows object tilting on the microscopic stage and leads to an improvement in localization precision and spatial resolution. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. By means of a special mounting device, test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range (corresponding here to an optical resolution of 10-30 nm) using standard microscopic equipment. As a proof of concept, the spindle apparatus of a mature mouse oocyte was imaged during metaphase II meiotic arrest under different perspectives. Only very few images registered under different rotation angles are sufficient for full 3D reconstruction. The results indicate the principal advantage of the micro axial tomography approach for many microscopic setups therein and also those of improved resolutions as obtained by high precision localization determination.

Application of dot-matrix illumination of liquid crystal phase space light modulator in 3D imaging of APD array

NASA Astrophysics Data System (ADS)

Wang, Shuai; Sun, Huayan; Guo, Huichao

2018-01-01

Aiming at the problem of beam scanning in low-resolution APD array in three-dimensional imaging, a method of beam scanning with liquid crystal phase-space optical modulator is proposed to realize high-resolution imaging by low-resolution APD array. First, a liquid crystal phase spatial light modulator is used to generate a beam array and then a beam array is scanned. Since the sub-beam divergence angle in the beam array is smaller than the field angle of a single pixel in the APD array, the APD's pixels respond only to the three-dimensional information of the beam illumination position. Through the scanning of the beam array, a single pixel is used to collect the target three-dimensional information multiple times, thereby improving the resolution of the APD detector. Finally, MATLAB is used to simulate the algorithm in this paper by using two-dimensional scalar diffraction theory, which realizes the splitting and scanning with a resolution of 5 x 5. The feasibility is verified theoretically.

High-resolution computer-generated reflection holograms with three-dimensional effects written directly on a silicon surface by a femtosecond laser.

PubMed

Wædegaard, Kristian J; Balling, Peter

2011-02-14

An infrared femtosecond laser has been used to write computer-generated holograms directly on a silicon surface. The high resolution offered by short-pulse laser ablation is employed to write highly detailed holograms with resolution up to 111 kpixels/mm2. It is demonstrated how three-dimensional effects can be realized in computer-generated holograms. Three-dimensional effects are visualized as a relative motion between different parts of the holographic reconstruction, when the hologram is moved relative to the reconstructing laser beam. Potential security applications are briefly discussed.

Concept for maritime near-surface surveillance using water Raman scattering

DOE PAGES

Shokair, Isaac R.; Johnson, Mark S.; Schmitt, Randal L.; ...

2018-06-08

Here, we discuss a maritime surveillance and detection concept based on Raman scattering of water molecules. Using a range-gated scanning lidar that detects Raman scattered photons from water, the absence or change of signal indicates the presence of a non-water object. With sufficient spatial resolution, a two-dimensional outline of the object can be generated by the scanning lidar. Because Raman scattering is an inelastic process with a relatively large wavelength shift for water, this concept avoids the often problematic elastic scattering for objects at or very close to the water surface or from the bottom surface for shallow waters. Themore » maximum detection depth for this concept is limited by the attenuation of the excitation and return Raman light in water. If excitation in the UV is used, fluorescence can be used for discrimination between organic and non-organic objects. In this paper, we present a lidar model for this concept and discuss results of proof-of-concept measurements. Using published cross section values, the model and measurements are in reasonable agreement and show that a sufficient number of Raman photons can be generated for modest lidar parameters to make this concept useful for near-surface detection.« less

Concept for maritime near-surface surveillance using water Raman scattering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shokair, Isaac R.; Johnson, Mark S.; Schmitt, Randal L.

Here, we discuss a maritime surveillance and detection concept based on Raman scattering of water molecules. Using a range-gated scanning lidar that detects Raman scattered photons from water, the absence or change of signal indicates the presence of a non-water object. With sufficient spatial resolution, a two-dimensional outline of the object can be generated by the scanning lidar. Because Raman scattering is an inelastic process with a relatively large wavelength shift for water, this concept avoids the often problematic elastic scattering for objects at or very close to the water surface or from the bottom surface for shallow waters. Themore » maximum detection depth for this concept is limited by the attenuation of the excitation and return Raman light in water. If excitation in the UV is used, fluorescence can be used for discrimination between organic and non-organic objects. In this paper, we present a lidar model for this concept and discuss results of proof-of-concept measurements. Using published cross section values, the model and measurements are in reasonable agreement and show that a sufficient number of Raman photons can be generated for modest lidar parameters to make this concept useful for near-surface detection.« less

Data center thermal management

DOEpatents

Hamann, Hendrik F.; Li, Hongfei

2016-02-09

Historical high-spatial-resolution temperature data and dynamic temperature sensor measurement data may be used to predict temperature. A first formulation may be derived based on the historical high-spatial-resolution temperature data for determining a temperature at any point in 3-dimensional space. The dynamic temperature sensor measurement data may be calibrated based on the historical high-spatial-resolution temperature data at a corresponding historical time. Sensor temperature data at a plurality of sensor locations may be predicted for a future time based on the calibrated dynamic temperature sensor measurement data. A three-dimensional temperature spatial distribution associated with the future time may be generated based on the forecasted sensor temperature data and the first formulation. The three-dimensional temperature spatial distribution associated with the future time may be projected to a two-dimensional temperature distribution, and temperature in the future time for a selected space location may be forecasted dynamically based on said two-dimensional temperature distribution.

A Multi-Resolution Data Structure for Two-Dimensional Morse Functions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bremer, P-T; Edelsbrunner, H; Hamann, B

2003-07-30

The efficient construction of simplified models is a central problem in the field of visualization. We combine topological and geometric methods to construct a multi-resolution data structure for functions over two-dimensional domains. Starting with the Morse-Smale complex we build a hierarchy by progressively canceling critical points in pairs. The data structure supports mesh traversal operations similar to traditional multi-resolution representations.

Global transport calculations with an equivalent barotropic system

NASA Technical Reports Server (NTRS)

Salby, Murry L.; O'Sullivan, Donal; Garcia, Rolando R.; Tribbia, Joseph

1990-01-01

Transport properties of the two-dimensional equations governing equivalent barotropic motion are investigated on the sphere. This system has ingredients such as forcing, equivalent depth, and thermal dissipation explicitly represented, and takes into account compression effects associated with vertical motion along isentropic surfaces. Horizontal transport properties of this system are investigated under adiabatic and diabatic conditions for different forms of dissipation, and over a range of resolutions. It is shown that forcing represetative of time-mean and amplified conditions at 10 mb leads to the behavior typical of observations at this level. The displacement of the polar night vortex and its distortion into a comma shape are evident, as is irreversible mixing under sufficiently strong forcing amplitude. It is shown that thermal dissipation influences the behavior significantly by inhibiting the amplification of unstable eddies and thereby the horizontal stirring of air.

Fixture-abutment connection surface and micro-gap measurements by 3D micro-tomographic technique analysis.

PubMed

Meleo, Deborah; Baggi, Luigi; Di Girolamo, Michele; Di Carlo, Fabio; Pecci, Raffaella; Bedini, Rossella

2012-01-01

X-ray micro-tomography (micro-CT) is a miniaturized form of conventional computed axial tomography (CAT) able to investigate small radio-opaque objects at a-few-microns high resolution, in a non-destructive, non-invasive, and tri-dimensional way. Compared to traditional optical and electron microscopy techniques, which provide two-dimensional images, this innovative investigation technology enables a sample tri-dimensional analysis without cutting, coating or exposing the object to any particular chemical treatment. X-ray micro-tomography matches ideal 3D microscopy features: the possibility of investigating an object in natural conditions and without any preparation or alteration; non-invasive, non-destructive, and sufficiently magnified 3D reconstruction; reliable measurement of numeric data of the internal structure (morphology, structure and ultra-structure). Hence, this technique has multi-fold applications in a wide range of fields, not only in medical and odontostomatologic areas, but also in biomedical engineering, materials science, biology, electronics, geology, archaeology, oil industry, and semi-conductors industry. This study shows possible applications of micro-CT in dental implantology to analyze 3D micro-features of dental implant to abutment interface. Indeed, implant-abutment misfit is known to increase mechanical stress on connection structures and surrounding bone tissue. This condition may cause not only screw preload loss or screw fracture, but also biological issues in peri-implant tissues.

Investigating Hydrocarbon Seep Environments with High-Resolution, Three-Dimensional Geographic Visualizations.

NASA Astrophysics Data System (ADS)

Doolittle, D. F.; Gharib, J. J.; Mitchell, G. A.

2015-12-01

Detailed photographic imagery and bathymetric maps of the seafloor acquired by deep submergence vehicles such as Autonomous Underwater Vehicles (AUV) and Remotely Operated Vehicles (ROV) are expanding how scientists and the public view and ultimately understand the seafloor and the processes that modify it. Several recently acquired optical and acoustic datasets, collected during ECOGIG (Ecosystem Impacts of Oil and Gas Inputs to the Gulf) and other Gulf of Mexico expeditions using the National Institute for Undersea Science Technology (NIUST) Eagle Ray, and Mola Mola AUVs, have been fused with lower resolution data to create unique three-dimensional geovisualizations. Included in these data are multi-scale and multi-resolution visualizations over hydrocarbon seeps and seep related features. Resolution of the data range from 10s of mm to 10s of m. When multi-resolution data is integrated into a single three-dimensional visual environment, new insights into seafloor and seep processes can be obtained from the intuitive nature of three-dimensional data exploration. We provide examples and demonstrate how integration of multibeam bathymetry, seafloor backscatter data, sub-bottom profiler data, textured photomosaics, and hull-mounted multibeam acoustic midwater imagery are made into a series a three-dimensional geovisualizations of actively seeping sites and associated chemosynthetic communities. From these combined and merged datasets, insights on seep community structure, morphology, ecology, fluid migration dynamics, and process geomorphology can be investigated from new spatial perspectives. Such datasets also promote valuable inter-comparisons of sensor resolution and performance.

Novel 16-channel receive coil array for accelerated upper airway MRI at 3 Tesla.

PubMed

Kim, Yoon-Chul; Hayes, Cecil E; Narayanan, Shrikanth S; Nayak, Krishna S

2011-06-01

Upper airway MRI can provide a noninvasive assessment of speech and swallowing disorders and sleep apnea. Recent work has demonstrated the value of high-resolution three-dimensional imaging and dynamic two-dimensional imaging and the importance of further improvements in spatio-temporal resolution. The purpose of the study was to describe a novel 16-channel 3 Tesla receive coil that is highly sensitive to the human upper airway and investigate the performance of accelerated upper airway MRI with the coil. In three-dimensional imaging of the upper airway during static posture, 6-fold acceleration is demonstrated using parallel imaging, potentially leading to capturing a whole three-dimensional vocal tract with 1.25 mm isotropic resolution within 9 sec of sustained sound production. Midsagittal spiral parallel imaging of vocal tract dynamics during natural speech production is demonstrated with 2 × 2 mm(2) in-plane spatial and 84 ms temporal resolution. Copyright © 2010 Wiley-Liss, Inc.

Scanning Backscatter Lidar Observations for Characterizing 4-D Cloud and Aerosol Fields to Improve Radiative Transfer Parameterizations

NASA Technical Reports Server (NTRS)

Schwemmer, Geary K.; Miller, David O.

2005-01-01

Clouds have a powerful influence on atmospheric radiative transfer and hence are crucial to understanding and interpreting the exchange of radiation between the Earth's surface, the atmosphere, and space. Because clouds are highly variable in space, time and physical makeup, it is important to be able to observe them in three dimensions (3-D) with sufficient resolution that the data can be used to generate and validate parameterizations of cloud fields at the resolution scale of global climate models (GCMs). Simulation of photon transport in three dimensionally inhomogeneous cloud fields show that spatial inhomogeneities tend to decrease cloud reflection and absorption and increase direct and diffuse transmission, Therefore it is an important task to characterize cloud spatial structures in three dimensions on the scale of GCM grid elements. In order to validate cloud parameterizations that represent the ensemble, or mean and variance of cloud properties within a GCM grid element, measurements of the parameters must be obtained on a much finer scale so that the statistics on those measurements are truly representative. High spatial sampling resolution is required, on the order of 1 km or less. Since the radiation fields respond almost instantaneously to changes in the cloud field, and clouds changes occur on scales of seconds and less when viewed on scales of approximately 100m, the temporal resolution of cloud properties should be measured and characterized on second time scales. GCM time steps are typically on the order of an hour, but in order to obtain sufficient statistical representations of cloud properties in the parameterizations that are used as model inputs, averaged values of cloud properties should be calculated on time scales on the order of 10-100 s. The Holographic Airborne Rotating Lidar Instrument Experiment (HARLIE) provides exceptional temporal (100 ms) and spatial (30 m) resolution measurements of aerosol and cloud backscatter in three dimensions. HARLIE was used in a ground-based configuration in several recent field campaigns. Principal data products include aerosol backscatter profiles, boundary layer heights, entrainment zone thickness, cloud fraction as a function of altitude and horizontal wind vector profiles based on correlating the motions of clouds and aerosol structures across portions of the scan. Comparisons will be made between various cloud detecting instruments to develop a baseline performance metric.

GPU-accelerated two dimensional synthetic aperture focusing for photoacoustic microscopy

NASA Astrophysics Data System (ADS)

Liu, Siyu; Feng, Xiaohua; Gao, Fei; Jin, Haoran; Zhang, Ruochong; Luo, Yunqi; Zheng, Yuanjin

2018-02-01

Acoustic resolution photoacoustic microscopy (AR-PAM) generally suffers from limited depth of focus, which had been extended by synthetic aperture focusing techniques (SAFTs). However, for three dimensional AR-PAM, current one dimensional (1D) SAFT and its improved version like cross-shaped SAFT do not provide isotropic resolution in the lateral direction. The full potential of the SAFT remains to be tapped. To this end, two dimensional (2D) SAFT with fast computing architecture is proposed in this work. Explained by geometric modeling and Fourier acoustics theories, 2D-SAFT provide the narrowest post-focusing capability, thus to achieve best lateral resolution. Compared with previous 1D-SAFT techniques, the proposed 2D-SAFT improved the lateral resolution by at least 1.7 times and the signal-to-noise ratio (SNR) by about 10 dB in both simulation and experiments. Moreover, the improved 2D-SAFT algorithm is accelerated by a graphical processing unit that reduces the long period of reconstruction to only a few seconds. The proposed 2D-SAFT is demonstrated to outperform previous reported 1D SAFT in the aspects of improving the depth of focus, imaging resolution, and SNR with fast computational efficiency. This work facilitates future studies on in vivo deeper and high-resolution photoacoustic microscopy beyond several centimeters.

DOE Office of Scientific and Technical Information (OSTI.GOV)

McCoy, Michel; Archer, Bill; Hendrickson, Bruce

The Stockpile Stewardship Program (SSP) is an integrated technical program for maintaining the safety, surety, and reliability of the U.S. nuclear stockpile. The SSP uses nuclear test data, computational modeling and simulation, and experimental facilities to advance understanding of nuclear weapons. It includes stockpile surveillance, experimental research, development and engineering programs, and an appropriately scaled production capability to support stockpile requirements. This integrated national program requires the continued use of experimental facilities and programs, and the computational capabilities to support these programs. The Advanced Simulation and Computing Program (ASC) is a cornerstone of the SSP, providing simulation capabilities and computationalmore » resources that support annual stockpile assessment and certification, study advanced nuclear weapons design and manufacturing processes, analyze accident scenarios and weapons aging, and provide the tools to enable stockpile Life Extension Programs (LEPs) and the resolution of Significant Finding Investigations (SFIs). This requires a balance of resource, including technical staff, hardware, simulation software, and computer science solutions. ASC is now focused on increasing predictive capabilities in a three-dimensional (3D) simulation environment while maintaining support to the SSP. The program continues to improve its unique tools for solving progressively more difficult stockpile problems (sufficient resolution, dimensionality, and scientific details), and quantifying critical margins and uncertainties. Resolving each issue requires increasingly difficult analyses because the aging process has progressively moved the stockpile further away from the original test base. Where possible, the program also enables the use of high performance computing (HPC) and simulation tools to address broader national security needs, such as foreign nuclear weapon assessments and counter nuclear terrorism.« less

Advanced Simulation & Computing FY15 Implementation Plan Volume 2, Rev. 0.5

DOE Office of Scientific and Technical Information (OSTI.GOV)

McCoy, Michel; Archer, Bill; Matzen, M. Keith

2014-09-16

The Stockpile Stewardship Program (SSP) is a single, highly integrated technical program for maintaining the surety and reliability of the U.S. nuclear stockpile. The SSP uses nuclear test data, computational modeling and simulation, and experimental facilities to advance understanding of nuclear weapons. It includes stockpile surveillance, experimental research, development and engineering programs, and an appropriately scaled production capability to support stockpile requirements. This integrated national program requires the continued use of experimental facilities and programs, and the computational enhancements to support these programs. The Advanced Simulation and Computing Program (ASC) is a cornerstone of the SSP, providing simulation capabilities andmore » computational resources that support annual stockpile assessment and certification, study advanced nuclear weapons design and manufacturing processes, analyze accident scenarios and weapons aging, and provide the tools to enable stockpile Life Extension Programs (LEPs) and the resolution of Significant Finding Investigations (SFIs). This requires a balance of resource, including technical staff, hardware, simulation software, and computer science solutions. As the program approaches the end of its second decade, ASC is intently focused on increasing predictive capabilities in a three-dimensional (3D) simulation environment while maintaining support to the SSP. The program continues to improve its unique tools for solving progressively more difficult stockpile problems (sufficient resolution, dimensionality, and scientific details), quantify critical margins and uncertainties, and resolve increasingly difficult analyses needed for the SSP. Where possible, the program also enables the use of high-performance simulation and computing tools to address broader national security needs, such as foreign nuclear weapon assessments and counternuclear terrorism.« less

Imaging Pathways in Fractured Rock Using Three-Dimensional Electrical Resistivity Tomography.

PubMed

Robinson, Judith; Slater, Lee; Johnson, Timothy; Shapiro, Allen; Tiedeman, Claire; Ntarlagiannis, Dimitrios; Johnson, Carole; Day-Lewis, Frederick; Lacombe, Pierre; Imbrigiotta, Thomas; Lane, John

2016-03-01

Major challenges exist in delineating bedrock fracture zones because these cause abrupt changes in geological and hydrogeological properties over small distances. Borehole observations cannot sufficiently capture heterogeneity in these systems. Geophysical techniques offer the potential to image properties and processes in between boreholes. We used three-dimensional cross borehole electrical resistivity tomography (ERT) in a 9 m (diameter) × 15 m well field to capture high-resolution flow and transport processes in a fractured mudstone contaminated by chlorinated solvents, primarily trichloroethylene. Conductive (sodium bromide) and resistive (deionized water) injections were monitored in seven boreholes. Electrode arrays with isolation packers and fluid sampling ports were designed to enable acquisition of ERT measurements during pulsed tracer injections. Fracture zone locations and hydraulic pathways inferred from hydraulic head drawdown data were compared with electrical conductivity distributions from ERT measurements. Static ERT imaging has limited resolution to decipher individual fractures; however, these images showed alternating conductive and resistive zones, consistent with alternating laminated and massive mudstone units at the site. Tracer evolution and migration was clearly revealed in time-lapse ERT images and supported by in situ borehole vertical apparent conductivity profiles collected during the pulsed tracer test. While water samples provided important local information at the extraction borehole, ERT delineated tracer migration over spatial scales capturing the primary hydrogeological heterogeneity controlling flow and transport. The fate of these tracer injections at this scale could not have been quantified using borehole logging and/or borehole sampling methods alone. © 2015, National Ground Water Association.

GPU Implementation of High Rayleigh Number Three-Dimensional Mantle Convection

NASA Astrophysics Data System (ADS)

Sanchez, D. A.; Yuen, D. A.; Wright, G. B.; Barnett, G. A.

2010-12-01

Although we have entered the age of petascale computing, many factors are still prohibiting high-performance computing (HPC) from infiltrating all suitable scientific disciplines. For this reason and others, application of GPU to HPC is gaining traction in the scientific world. With its low price point, high performance potential, and competitive scalability, GPU has been an option well worth considering for the last few years. Moreover with the advent of NVIDIA's Fermi architecture, which brings ECC memory, better double-precision performance, and more RAM to GPU, there is a strong message of corporate support for GPU in HPC. However many doubts linger concerning the practicality of using GPU for scientific computing. In particular, GPU has a reputation for being difficult to program and suitable for only a small subset of problems. Although inroads have been made in addressing these concerns, for many scientists GPU still has hurdles to clear before becoming an acceptable choice. We explore the applicability of GPU to geophysics by implementing a three-dimensional, second-order finite-difference model of Rayleigh-Benard thermal convection on an NVIDIA GPU using C for CUDA. Our code reaches sufficient resolution, on the order of 500x500x250 evenly-spaced finite-difference gridpoints, on a single GPU. We make extensive use of highly optimized CUBLAS routines, allowing us to achieve performance on the order of O( 0.1 ) µs per timestep*gridpoint at this resolution. This performance has allowed us to study high Rayleigh number simulations, on the order of 2x10^7, on a single GPU.

Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography.

PubMed

Wojtkowski, Maciej; Srinivasan, Vivek; Fujimoto, James G; Ko, Tony; Schuman, Joel S; Kowalczyk, Andrzej; Duker, Jay S

2005-10-01

To demonstrate high-speed, ultrahigh-resolution, 3-dimensional optical coherence tomography (3D OCT) and new protocols for retinal imaging. Ultrahigh-resolution OCT using broadband light sources achieves axial image resolutions of approximately 2 microm compared with standard 10-microm-resolution OCT current commercial instruments. High-speed OCT using spectral/Fourier domain detection enables dramatic increases in imaging speeds. Three-dimensional OCT retinal imaging is performed in normal human subjects using high-speed ultrahigh-resolution OCT. Three-dimensional OCT data of the macula and optic disc are acquired using a dense raster scan pattern. New processing and display methods for generating virtual OCT fundus images; cross-sectional OCT images with arbitrary orientations; quantitative maps of retinal, nerve fiber layer, and other intraretinal layer thicknesses; and optic nerve head topographic parameters are demonstrated. Three-dimensional OCT imaging enables new imaging protocols that improve visualization and mapping of retinal microstructure. An OCT fundus image can be generated directly from the 3D OCT data, which enables precise and repeatable registration of cross-sectional OCT images and thickness maps with fundus features. Optical coherence tomography images with arbitrary orientations, such as circumpapillary scans, can be generated from 3D OCT data. Mapping of total retinal thickness and thicknesses of the nerve fiber layer, photoreceptor layer, and other intraretinal layers is demonstrated. Measurement of optic nerve head topography and disc parameters is also possible. Three-dimensional OCT enables measurements that are similar to those of standard instruments, including the StratusOCT, GDx, HRT, and RTA. Three-dimensional OCT imaging can be performed using high-speed ultrahigh-resolution OCT. Three-dimensional OCT provides comprehensive visualization and mapping of retinal microstructures. The high data acquisition speeds enable high-density data sets with large numbers of transverse positions on the retina, which reduces the possibility of missing focal pathologies. In addition to providing image information such as OCT cross-sectional images, OCT fundus images, and 3D rendering, quantitative measurement and mapping of intraretinal layer thickness and topographic features of the optic disc are possible. We hope that 3D OCT imaging may help to elucidate the structural changes associated with retinal disease as well as improve early diagnosis and monitoring of disease progression and response to treatment.

Current singularities at quasi-separatrix layers and three-dimensional magnetic nulls

DOE Office of Scientific and Technical Information (OSTI.GOV)

Craig, I. J. D.; Effenberger, Frederic, E-mail: feffen@waikato.ac.nz

2014-11-10

The open problem of how singular current structures form in line-tied, three-dimensional magnetic fields is addressed. A Lagrangian magneto-frictional relaxation method is employed to model the field evolution toward the final near-singular state. Our starting point is an exact force-free solution of the governing magnetohydrodynamic equations that is sufficiently general to allow for topological features like magnetic nulls to be inside or outside the computational domain, depending on a simple set of parameters. Quasi-separatrix layers (QSLs) are present in these structures and, together with the magnetic nulls, they significantly influence the accumulation of current. It is shown that perturbations affectingmore » the lateral boundaries of the configuration lead not only to collapse around the magnetic null but also to significant QSL currents. Our results show that once a magnetic null is present, the developing currents are always attracted to that specific location and show a much stronger scaling with resolution than the currents that form along the QSL. In particular, the null-point scalings can be consistent with models of 'fast' reconnection. The QSL currents also appear to be unbounded but give rise to weaker singularities, independent of the perturbation amplitude.« less

Photography in Dermatologic Surgery: Selection of an Appropriate Camera Type for a Particular Clinical Application.

PubMed

Chen, Brian R; Poon, Emily; Alam, Murad

2017-08-01

Photographs are an essential tool for the documentation and sharing of findings in dermatologic surgery, and various camera types are available. To evaluate the currently available camera types in view of the special functional needs of procedural dermatologists. Mobile phone, point and shoot, digital single-lens reflex (DSLR), digital medium format, and 3-dimensional cameras were compared in terms of their usefulness for dermatologic surgeons. For each camera type, the image quality, as well as the other practical benefits and limitations, were evaluated with reference to a set of ideal camera characteristics. Based on these assessments, recommendations were made regarding the specific clinical circumstances in which each camera type would likely be most useful. Mobile photography may be adequate when ease of use, availability, and accessibility are prioritized. Point and shoot cameras and DSLR cameras provide sufficient resolution for a range of clinical circumstances, while providing the added benefit of portability. Digital medium format cameras offer the highest image quality, with accurate color rendition and greater color depth. Three-dimensional imaging may be optimal for the definition of skin contour. The selection of an optimal camera depends on the context in which it will be used.

A three-dimensional ParF meshwork assembles through the nucleoid to mediate plasmid segregation

PubMed Central

McLeod, Brett N.; Allison-Gamble, Gina E.; Barge, Madhuri T.; Tonthat, Nam K.; Schumacher, Maria A.; Hayes, Finbarr

2017-01-01

Abstract Genome segregation is a fundamental step in the life cycle of every cell. Most bacteria rely on dedicated DNA partition proteins to actively segregate chromosomes and low copy-number plasmids. Here, by employing super resolution microscopy, we establish that the ParF DNA partition protein of the ParA family assembles into a three-dimensional meshwork that uses the nucleoid as a scaffold and periodically shuttles between its poles. Whereas ParF specifies the territory for plasmid trafficking, the ParG partner protein dictates the tempo of ParF assembly cycles and plasmid segregation events by stimulating ParF adenosine triphosphate hydrolysis. Mutants in which this ParG temporal regulation is ablated show partition deficient phenotypes as a result of either altered ParF structure or dynamics and indicate that ParF nucleoid localization and dynamic relocation, although necessary, are not sufficient per se to ensure plasmid segregation. We propose a Venus flytrap model that merges the concepts of ParA polymerization and gradient formation and speculate that a transient, dynamic network of intersecting polymers that branches into the nucleoid interior is a widespread mechanism to distribute sizeable cargos within prokaryotic cells. PMID:28034957

The effects of environmental variability and spatial sampling on the three-dimensional inversion problem.

PubMed

Bender, Christopher M; Ballard, Megan S; Wilson, Preston S

2014-06-01

The overall goal of this work is to quantify the effects of environmental variability and spatial sampling on the accuracy and uncertainty of estimates of the three-dimensional ocean sound-speed field. In this work, ocean sound speed estimates are obtained with acoustic data measured by a sparse autonomous observing system using a perturbative inversion scheme [Rajan, Lynch, and Frisk, J. Acoust. Soc. Am. 82, 998-1017 (1987)]. The vertical and horizontal resolution of the solution depends on the bandwidth of acoustic data and on the quantity of sources and receivers, respectively. Thus, for a simple, range-independent ocean sound speed profile, a single source-receiver pair is sufficient to estimate the water-column sound-speed field. On the other hand, an environment with significant variability may not be fully characterized by a large number of sources and receivers, resulting in uncertainty in the solution. This work explores the interrelated effects of environmental variability and spatial sampling on the accuracy and uncertainty of the inversion solution though a set of case studies. Synthetic data representative of the ocean variability on the New Jersey shelf are used.

Size effects under homogeneous deformation of single crystals: A discrete dislocation analysis

NASA Astrophysics Data System (ADS)

Guruprasad, P. J.; Benzerga, A. A.

Mechanism-based discrete dislocation plasticity is used to investigate the effect of size on micron scale crystal plasticity under conditions of macroscopically homogeneous deformation. Long-range interactions among dislocations are naturally incorporated through elasticity. Constitutive rules are used which account for key short-range dislocation interactions. These include junction formation and dynamic source and obstacle creation. Two-dimensional calculations are carried out which can handle high dislocation densities and large strains up to 0.1. The focus is laid on the effect of dimensional constraints on plastic flow and hardening processes. Specimen dimensions ranging from hundreds of nanometers to tens of microns are considered. Our findings show a strong size-dependence of flow strength and work-hardening rate at the micron scale. Taylor-like hardening is shown to be insufficient as a rationale for the flow stress scaling with specimen dimensions. The predicted size effect is associated with the emergence, at sufficient resolution, of a signed dislocation density. Heuristic correlations between macroscopic flow stress and macroscopic measures of dislocation density are sought. Most accurate among those is a correlation based on two state variables: the total dislocation density and an effective, scale-dependent measure of signed density.

High-Resolution Large Field-of-View FUV Compact Camera

NASA Technical Reports Server (NTRS)

Spann, James F.

2006-01-01

The need for a high resolution camera with a large field of view and capable to image dim emissions in the far-ultraviolet is driven by the widely varying intensities of FUV emissions and spatial/temporal scales of phenomena of interest in the Earth% ionosphere. In this paper, the concept of a camera is presented that is designed to achieve these goals in a lightweight package with sufficient visible light rejection to be useful for dayside and nightside emissions. The camera employs the concept of self-filtering to achieve good spectral resolution tuned to specific wavelengths. The large field of view is sufficient to image the Earth's disk at Geosynchronous altitudes and capable of a spatial resolution of >20 km. The optics and filters are emphasized.

Grating-based tomography applications in biomedical engineering

NASA Astrophysics Data System (ADS)

Schulz, Georg; Thalmann, Peter; Khimchenko, Anna; Müller, Bert

2017-10-01

For the investigation of soft tissues or tissues consisting of soft and hard tissues on the microscopic level, hard X-ray phase tomography has become one of the most suitable imaging techniques. Besides other phase contrast methods grating interferometry has the advantage of higher sensitivity than inline methods and the quantitative results. One disadvantage of the conventional double-grating setup (XDGI) compared to inline methods is the limitation of the spatial resolution. This limitation can be overcome by removing the analyser grating resulting in a single-grating setup (XSGI). In order to verify the performance of XSGI concerning contrast and spatial resolution, a quantitative comparison of XSGI and XDGI tomograms of a human nerve was performed. Both techniques provide sufficient contrast to allow for the distinction of tissue types. The spatial resolution of the two-fold binned XSGI data set is improved by a factor of two in comparison to XDGI which underlies its performance in tomography of soft tissues. Another application for grating-based X-ray phase tomography is the simultaneous visualization of soft and hard tissues of a plaque-containing coronary artery. The simultaneous visualization of both tissues is important for the segmentation of the lumen. The segmented data can be used for flow simulations in order to obtain information about the three-dimensional wall shear stress distribution needed for the optimization of mechano-sensitive nanocontainers used for drug delivery.

Structure of myosin filaments from relaxed Lethocerus flight muscle by cryo-EM at 6 Å resolution

PubMed Central

Hu, Zhongjun; Taylor, Dianne W.; Reedy, Michael K.; Edwards, Robert J.; Taylor, Kenneth A.

2016-01-01

We describe a cryo–electron microscopy three-dimensional image reconstruction of relaxed myosin II–containing thick filaments from the flight muscle of the giant water bug Lethocerus indicus. The relaxed thick filament structure is a key element of muscle physiology because it facilitates the reextension process following contraction. Conversely, the myosin heads must disrupt their relaxed arrangement to drive contraction. Previous models predicted that Lethocerus myosin was unique in having an intermolecular head-head interaction, as opposed to the intramolecular head-head interaction observed in all other species. In contrast to the predicted model, we find an intramolecular head-head interaction, which is similar to that of other thick filaments but oriented in a distinctly different way. The arrangement of myosin’s long α-helical coiled-coil rod domain has been hypothesized as either curved layers or helical subfilaments. Our reconstruction is the first report having sufficient resolution to track the rod α helices in their native environment at resolutions ~5.5 Å, and it shows that the layer arrangement is correct for Lethocerus. Threading separate paths through the forest of myosin coiled coils are four nonmyosin peptides. We suggest that the unusual position of the heads and the rod arrangement separated by nonmyosin peptides are adaptations for mechanical signal transduction whereby applied tension disrupts the myosin heads as a component of stretch activation. PMID:27704041

Molecular characterization of phytoplankton dissolved organic matter (DOM) and sulfur components using high resolution Orbitrap mass spectrometry.

PubMed

Mangal, Vaughn; Stock, Naomi L; Guéguen, Celine

2016-03-01

Orbitrap high resolution mass spectrometry (HRMS) with electrospray ionization in both positive and negative polarity was conducted on Suwannee River fulvic acid (SRFA), Pony Lake fulvic acid (PLFA) standards, and dissolved organic matter (DOM) released by freshwater phytoplankton (Scenedesmus obliquus, Euglena mutabilis, and Euglena gracilis). Three-dimensional van Krevelen diagrams expressing various oxygenation states of sulfur molecules and abundance plots of sulfur-containing species were constructed. Orbitrap HRMS analysis of SRFA found a high density of peaks in the lignin region (77 %) and low density of protein material (6.53 %), whereas for PLFA, 25 % of the total peaks were lignin related compared to 56 % of peaks in protein regions, comparable with other HRMS studies. Phytoplankton-derived DOM of S. obliquus, E. mutabilis, and E. gracilis was dominated by protein molecules at respective percentages of 36, 46, and 49 %, and is consistent with previous experiments examining phytoplankton-derived DOM composition. The normalized percentage of SO-containing compounds was determined among the three phytoplankton to be 56 % for Scenedesmus, 54 % for E. mutabilis, and 47 % for E. gracilis, suggesting variation between sulfur content in phytoplankton-derived DOM and differences in metal binding capacities. These results suggest the level of resolution by Orbitrap mass spectrometry is sufficient for preliminary characterization of phytoplankton DOM at an affordable cost relative to other HRMS techniques.

High-resolution two dimensional advective transport

USGS Publications Warehouse

Smith, P.E.; Larock, B.E.

1989-01-01

The paper describes a two-dimensional high-resolution scheme for advective transport that is based on a Eulerian-Lagrangian method with a flux limiter. The scheme is applied to the problem of pure-advection of a rotated Gaussian hill and shown to preserve the monotonicity property of the governing conservation law.

Fast integrated intravascular photoacoustic/ultrasound catheter

NASA Astrophysics Data System (ADS)

Choi, Changhoon; Cho, Seunghee; Kim, Taehoon; Park, Sungjo; Park, Hyoeun; Kim, Jinmoo; Lee, Seunghoon; Kang, Yeonsu; Jang, Kiyuk; Kim, Chulhong

2016-03-01

In cardiology, a vulnerable plaque is considered to be a key subject because it is strongly related to atherosclerosis and acute myocardial infarction. Because conventional intravascular imaging devices exhibit several limitations with regard to vulnerable plaque detection, the need for an effective lipid imaging modality has been continuously suggested. Photoacoustic (PA) imaging is a medical imaging technique with a high level of ultrasound (US) resolution and strong optical contrast. In this study, we successfully developed an integrated intravascular photoacoustic/ultrasound (IV-PAUS) imaging system with a catheter diameter of 1.2 mm for lipid-rich atherosclerosis imaging. An Nd:YAG pulsed laser with an excitation wavelength of 1064 nm was utilized. IV-PAUS offers 5-mm depth penetration and axial and lateral PA imaging resolutions of 94 μm and 203 μm, respectively, as determined by imaging a 6-μm carbon fiber. We initially obtained 3-dimensional (3D) co-registered PA/US images of metal stents. Subsequently, we successfully obtained 3D coregistered PA/US ex vivo images using an iliac artery from a rabbit atherosclerosis model. Accordingly, lipid-rich plaques were sufficiently differentiated from normal tissue in the ex vivo experiment. We validated these findings histologically to confirm the lipid content.

Quantifying Melt Ponds in the Beaufort MIZ using Linear Support Vector Machines from High Resolution Panchromatic Images

NASA Astrophysics Data System (ADS)

Ortiz, M.; Graber, H. C.; Wilkinson, J.; Nyman, L. M.; Lund, B.

2017-12-01

Much work has been done on determining changes in summer ice albedo and morphological properties of melt ponds such as depth, shape and distribution using in-situ measurements and satellite-based sensors. Although these studies have dedicated much pioneering work in this area, there still lacks sufficient spatial and temporal scales. We present a prototype algorithm using Linear Support Vector Machines (LSVMs) designed to quantify the evolution of melt pond fraction from a recently government-declassified high-resolution panchromatic optical dataset. The study area of interest lies within the Beaufort marginal ice zone (MIZ), where several in-situ instruments were deployed by the British Antarctic Survey in joint with the MIZ Program, from April-September, 2014. The LSVM uses four dimensional feature data from the intensity image itself, and from various textures calculated from a modified first-order histogram technique using probability density of occurrences. We explore both the temporal evolution of melt ponds and spatial statistics such as pond fraction, pond area, and number pond density, to name a few. We also introduce a linear regression model that can potentially be used to estimate average pond area by ingesting several melt pond statistics and shape parameters.

Serial block face-scanning electron microscopy: a tool for studying embryonic development at the cell-matrix interface.

PubMed

Starborg, Tobias; Kadler, Karl E

2015-03-01

Studies of gene regulation, signaling pathways, and stem cell biology are contributing greatly to our understanding of early embryonic vertebrate development. However, much less is known about the events during the latter half of embryonic development, when tissues comprising mostly extracellular matrix (ECM) are formed. The matrix extends far beyond the boundaries of individual cells and is refractory to study by conventional biochemical and molecular techniques; thus major gaps exist in our knowledge of the formation and three-dimensional (3D) organization of the dense tissues that form the bulk of adult vertebrates. Serial block face-scanning electron microscopy (SBF-SEM) has the ability to image volumes of tissue containing numerous cells at a resolution sufficient to study the organization of the ECM. Furthermore, whereas light microscopy was once relatively straightforward and electron microscopy was performed in specialist laboratories, the tables are turned; SBF-SEM is relatively straightforward and is becoming routine in high-end resolution studies of embryonic structures in vivo. In this review, we discuss the emergence of SBF-SEM as a tool for studying embryonic vertebrate development. © 2015 Wiley Periodicals, Inc.

Assessing the value of different data sets and modeling schemes for flow and transport simulations

NASA Astrophysics Data System (ADS)

Hyndman, D. W.; Dogan, M.; Van Dam, R. L.; Meerschaert, M. M.; Butler, J. J., Jr.; Benson, D. A.

2014-12-01

Accurate modeling of contaminant transport has been hampered by an inability to characterize subsurface flow and transport properties at a sufficiently high resolution. However mathematical extrapolation combined with different measurement methods can provide realistic three-dimensional fields of highly heterogeneous hydraulic conductivity (K). This study demonstrates an approach to evaluate the time, cost, and efficiency of subsurface K characterization. We quantify the value of different data sets at the highly heterogeneous Macro Dispersion Experiment (MADE) Site in Mississippi, which is a flagship test site that has been used for several macro- and small-scale tracer tests that revealed non-Gaussian tracer behavior. Tracer data collected at the site are compared to models that are based on different types and resolution of geophysical and hydrologic data. We present a cost-benefit analysis of several techniques including: 1) flowmeter K data, 2) direct-push K data, 3) ground penetrating radar, and 4) two stochastic methods to generate K fields. This research provides an initial assessment of the level of data necessary to accurately simulate solute transport with the traditional advection dispersion equation; it also provides a basis to design lower cost and more efficient remediation schemes at highly heterogeneous sites.

3D DNS and LES of Breaking Inertia-Gravity Waves

NASA Astrophysics Data System (ADS)

Remmler, S.; Fruman, M. D.; Hickel, S.; Achatz, U.

2012-04-01

As inertia-gravity waves we refer to gravity waves that have a sufficiently low frequency and correspondingly large horizontal wavelength to be strongly influenced by the Coriolis force. Inertia-gravity waves are very active in the middle atmosphere and their breaking is potentially an important influence on the circulation in this region. The parametrization of this process requires a good theoretical understanding, which we want to enhance with the present study. Primary linear instabilities of an inertia-gravity wave and "2.5-dimensional" nonlinear simulations (where the spatial dependence is two dimensional but the velocity and vorticity fields are three-dimensional) with the wave perturbed by its leading primary instabilities by Achatz [1] have shown that the breaking differs significantly from that of high-frequency gravity waves due to the strongly sheared component of velocity perpendicular to the plane of wave-propagation. Fruman & Achatz [2] investigated the three-dimensionalization of the breaking by computing the secondary linear instabilities of the same waves using singular vector analysis. These secondary instabilities are variations perpendicular to the direction of the primary perturbation and the wave itself, and their wavelengths are an order of magnitude shorter than both. In continuation of this work, we carried out fully three-dimensional nonlinear simulations of inertia-gravity waves perturbed by their leading primary and secondary instabilities. The direct numerical simulation (DNS) was made tractable by restricting the domain size to the dominant scales selected by the linear analyses. The study includes both convectively stable and unstable waves. To the best of our knowledge, this is the first fully three-dimensional nonlinear direct numerical simulation of inertia-gravity waves at realistic Reynolds numbers with complete resolution of the smallest turbulence scales. Previous simulations either were restricted to high frequency gravity waves (e. g. Fritts et al. [3]), or the ratio N/f was artificially reduced (e. g. Lelong & Dunkerton [4]). The present simulations give us insight into the three-dimensional breaking process as well as the emerging turbulence. We assess the possibility of reducing the computational costs of three-dimensional simulations by using an implicit turbulence subgrid-scale parametrization based on the Adaptive Local Deconvolution Method (ALDM) for stratified turbulence [5]. In addition, we have performed ensembles of nonlinear 2.5-dimensional DNS, like those in Achatz [1] but with a small amount of noise superposed to the initial state, and compared the results with coarse-resolution simulations using either ALDM as well as with standard LES schemes. We found that the results of the models with parametrized turbulence, which are orders of magnitude more computationally economical than the DNS, compare favorably with the DNS in terms of the decay of the wave amplitude with time (the quantity most important for application to gravity-wave drag parametrization) suggesting that they may be trusted in future simulations of gravity wave breaking.

High spatial resolution three-dimensional mapping of vegetation spectral dynamics using computer vision

Treesearch

Jonathan P. Dandois; Erle C. Ellis

2013-01-01

High spatial resolution three-dimensional (3D) measurements of vegetation by remote sensing are advancing ecological research and environmental management. However, substantial economic and logistical costs limit this application, especially for observing phenological dynamics in ecosystem structure and spectral traits. Here we demonstrate a new aerial remote sensing...

Comparison Simulations of Gas Giant Planet Formation via Disk Instability

NASA Astrophysics Data System (ADS)

Pickett, Megan K.; Cai, K.; Durisen, R.; Milne, M.

2011-01-01

There has been disagreement about whether cooling in protoplanetary disks can be sufficiently fast to induce the formation of gas giant protoplanets via gravitational instabilities. Simulations by our own group and others indicate that this method of planet formation does not work for disks around young, low-mass stars inside several tens of AU, while simulations by other groups show fragmentation into protoplanetary clumps in this region. To allow direct comparison in hopes of isolating the cause of the differences, we here present a comparison high-resolution three-dimensional hydrodynamics simulation of a protoplanetary disk,using an improved version of one of our own radiative schemes. We find that the disk does not fragment in our code but instead quickly settles into a state with only low amplitude nonaxisymmetric structure, which persists for at least several outer disk rotations. Further, we see no rapid radiative or convective cooling.

[Extraction of buildings three-dimensional information from high-resolution satellite imagery based on Barista software].

PubMed

Zhang, Pei-feng; Hu, Yuan-man; He, Hong-shi

2010-05-01

The demand for accurate and up-to-date spatial information of urban buildings is becoming more and more important for urban planning, environmental protection, and other vocations. Today's commercial high-resolution satellite imagery offers the potential to extract the three-dimensional information of urban buildings. This paper extracted the three-dimensional information of urban buildings from QuickBird imagery, and validated the precision of the extraction based on Barista software. It was shown that the extraction of three-dimensional information of the buildings from high-resolution satellite imagery based on Barista software had the advantages of low professional level demand, powerful universality, simple operation, and high precision. One pixel level of point positioning and height determination accuracy could be achieved if the digital elevation model (DEM) and sensor orientation model had higher precision and the off-Nadir View Angle was relatively perfect.

Four dimensional magnetic resonance imaging with retrospective k-space reordering: A feasibility study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Yilin; Yin, Fang-Fang; Cai, Jing, E-mail: jing.cai@duke.edu

Purpose: Current four dimensional magnetic resonance imaging (4D-MRI) techniques lack sufficient temporal/spatial resolution and consistent tumor contrast. To overcome these limitations, this study presents the development and initial evaluation of a new strategy for 4D-MRI which is based on retrospective k-space reordering. Methods: We simulated a k-space reordered 4D-MRI on a 4D digital extended cardiac-torso (XCAT) human phantom. A 2D echo planar imaging MRI sequence [frame rate (F) = 0.448 Hz; image resolution (R) = 256 × 256; number of k-space segments (N{sub KS}) = 4] with sequential image acquisition mode was assumed for the simulation. Image quality of themore » simulated “4D-MRI” acquired from the XCAT phantom was qualitatively evaluated, and tumor motion trajectories were compared to input signals. In particular, mean absolute amplitude differences (D) and cross correlation coefficients (CC) were calculated. Furthermore, to evaluate the data sufficient condition for the new 4D-MRI technique, a comprehensive simulation study was performed using 30 cancer patients’ respiratory profiles to study the relationships between data completeness (C{sub p}) and a number of impacting factors: the number of repeated scans (N{sub R}), number of slices (N{sub S}), number of respiratory phase bins (N{sub P}), N{sub KS}, F, R, and initial respiratory phase at image acquisition (P{sub 0}). As a proof-of-concept, we implemented the proposed k-space reordering 4D-MRI technique on a T2-weighted fast spin echo MR sequence and tested it on a healthy volunteer. Results: The simulated 4D-MRI acquired from the XCAT phantom matched closely to the original XCAT images. Tumor motion trajectories measured from the simulated 4D-MRI matched well with input signals (D = 0.83 and 0.83 mm, and CC = 0.998 and 0.992 in superior–inferior and anterior–posterior directions, respectively). The relationship between C{sub p} and N{sub R} was found best represented by an exponential function (C{sub P}=100(1−e{sup −0.18N{sub R}}), when N{sub S} = 30, N{sub P} = 6). At a C{sub P} value of 95%, the relative error in tumor volume was 0.66%, indicating that N{sub R} at a C{sub P} value of 95% (N{sub R,95%}) is sufficient. It was found that N{sub R,95%} is approximately linearly proportional to N{sub P} (r = 0.99), and nearly independent of all other factors. The 4D-MRI images of the healthy volunteer clearly demonstrated respiratory motion in the diaphragm region with minimal motion induced noise or aliasing. Conclusions: It is feasible to generate respiratory correlated 4D-MRI by retrospectively reordering k-space based on respiratory phase. This new technology may lead to the next generation 4D-MRI with high spatiotemporal resolution and optimal tumor contrast, holding great promises to improve the motion management in radiotherapy of mobile cancers.« less

The feasibility study on 3-dimensional fluorescent x-ray computed tomography using the pinhole effect for biomedical applications.

PubMed

Sunaguchi, Naoki; Yuasa, Tetsuya; Hyodo, Kazuyuki; Zeniya, Tsutomu

2013-01-01

We propose a 3-dimensional fluorescent x-ray computed tomography (CT) pinhole collimator, aimed at providing molecular imaging with quantifiable measures and sub-millimeter spatial resolution. In this study, we demonstrate the feasibility of this concept and investigate imaging properties such as spatial resolution, contrast resolution and quantifiable measures, by imaging physical phantoms using a preliminary imaging system developed with monochromatic synchrotron x rays constructed at the BLNE-7A experimental line at KEK, Japan.

Contactless measurement of canine retraction by digital macrophotogrammetry during hybrid retractor application.

PubMed

Sander, Christian; Geiger, Martin; Sander, Franz-Günter

2002-11-01

A new contactless method for measuring tooth movements is presented. Digital macrophotogrammetry (DMP) enables the orthodontist to obtain information on the three-dimensional movement of a tooth at each session. Analysis of the DMP images provides information on the translation and toration of a tooth during treatment. It is conceivable that the introduction of DMP will make an important contribution to quality assurance.DMP application during canine retraction with the Hybrid Retractor((R)) has pave the way for the orthodontic appliance to be correctly adjusted at each session. Despite intra- and interindividual differences during canine movement, for which the anisotropy of the bone seems to be basically responsible, a movement velocity of ca 1.2 mm is to be expected over the entire treatment period. The preconditions for the three-dimensional orientation and description of canine movement are:1. The markers on the brackets and attachments must be clearly visible throughout the treatment.2. The teeth to which the scaling frame is fixed should not move during treatment.3. Four measuring points should be visible on each bracket.4. The control points on the frame must be positioned three-dimensionally, and it must be possible to determine their position with sufficient accuracy in advance. In vitro calibration of the photogrammetry presented here yielded a resolution of 1 micrometer for translation and of better than 1/10 degrees for rotation around the three spatial axes under optimal conditions.

Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells

PubMed Central

Rodriguez, Jose A.; Xu, Rui; Chen, Chien-Chun; Huang, Zhifeng; Jiang, Huaidong; Chen, Allan L.; Raines, Kevin S.; Pryor Jr, Alan; Nam, Daewoong; Wiegart, Lutz; Song, Changyong; Madsen, Anders; Chushkin, Yuriy; Zontone, Federico; Bradley, Peter J.; Miao, Jianwei

2015-01-01

A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres. PMID:26306199

Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells

DOE PAGES

Rodriguez, Jose A.; Xu, Rui; Chen, Chien -Chun; ...

2015-09-01

Here, a structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 Kev X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and themore » three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. Finally, it is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres.« less

Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells.

PubMed

Rodriguez, Jose A; Xu, Rui; Chen, Chien-Chun; Huang, Zhifeng; Jiang, Huaidong; Chen, Allan L; Raines, Kevin S; Pryor, Alan; Nam, Daewoong; Wiegart, Lutz; Song, Changyong; Madsen, Anders; Chushkin, Yuriy; Zontone, Federico; Bradley, Peter J; Miao, Jianwei

2015-09-01

A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres.

Feasibility of creating a high-resolution 3D diffusion tensor imaging based atlas of the human brainstem: A case study at 11.7T

PubMed Central

Aggarwal, Manisha; Zhang, Jiangyang; Pletnikova, Olga; Crain, Barbara; Troncoso, Juan; Mori, Susumu

2013-01-01

A three-dimensional stereotaxic atlas of the human brainstem based on high resolution ex vivo diffusion tensor imaging (DTI) is introduced. The atlas consists of high resolution (125–255 μm isotropic) three-dimensional DT images of the formalin-fixed brainstem acquired at 11.7T. The DTI data revealed microscopic neuroanatomical details, allowing three-dimensional visualization and reconstruction of fiber pathways including the decussation of the pyramidal tract fibers, and interdigitating fascicles of the corticospinal and transverse pontine fibers. Additionally, strong grey-white matter contrasts in the apparent diffusion coefficient (ADC) maps enabled precise delineation of grey matter nuclei in the brainstem, including the cranial nerve and the inferior olivary nuclei. Comparison with myelin-stained histology shows that at the level of resolution achieved in this study, the structural details resolved with DTI contrasts in the brainstem were comparable to anatomical delineation obtained with histological sectioning. Major neural structures delineated from DTI contrasts in the brainstem are segmented and three-dimensionally reconstructed. Further, the ex vivo DTI data are nonlinearly mapped to a widely-used in vivo human brain atlas, to construct a high-resolution atlas of the brainstem in the Montreal Neurological Institute (MNI) stereotaxic coordinate space. The results demonstrate the feasibility of developing a 3D DTI based atlas for detailed characterization of brainstem neuroanatomy with high resolution and contrasts, which will be a useful resource for research and clinical applications. PMID:23384518

Advanced Simulation and Computing Fiscal Year 14 Implementation Plan, Rev. 0.5

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meisner, Robert; McCoy, Michel; Archer, Bill

2013-09-11

The Stockpile Stewardship Program (SSP) is a single, highly integrated technical program for maintaining the surety and reliability of the U.S. nuclear stockpile. The SSP uses nuclear test data, computational modeling and simulation, and experimental facilities to advance understanding of nuclear weapons. It includes stockpile surveillance, experimental research, development and engineering programs, and an appropriately scaled production capability to support stockpile requirements. This integrated national program requires the continued use of experimental facilities and programs, and the computational enhancements to support these programs. The Advanced Simulation and Computing Program (ASC) is a cornerstone of the SSP, providing simulation capabilities andmore » computational resources that support annual stockpile assessment and certification, study advanced nuclear weapons design and manufacturing processes, analyze accident scenarios and weapons aging, and provide the tools to enable stockpile Life Extension Programs (LEPs) and the resolution of Significant Finding Investigations (SFIs). This requires a balanced resource, including technical staff, hardware, simulation software, and computer science solutions. In its first decade, the ASC strategy focused on demonstrating simulation capabilities of unprecedented scale in three spatial dimensions. In its second decade, ASC is now focused on increasing predictive capabilities in a three-dimensional (3D) simulation environment while maintaining support to the SSP. The program continues to improve its unique tools for solving progressively more difficult stockpile problems (sufficient resolution, dimensionality, and scientific details), quantify critical margins and uncertainties, and resolve increasingly difficult analyses needed for the SSP. Moreover, ASC’s business model is integrated and focused on requirements-driven products that address long-standing technical questions related to enhanced predictive capability in the simulation tools.« less

Evaluating interception of larval pallid sturgeon on the Lower Missouri River- data acquisition, interpolation, and visualization

NASA Astrophysics Data System (ADS)

Bulliner, E. A., IV; Erwin, S. O.; Anderson, B. J.; Wilson, H.; Jacobson, R. B.

2016-12-01

The transition from endogenous to exogenous feeding is an important life-stage transition for many riverine fish larvae. On the Missouri River, U.S., riverine alteration has decreased connectivity between the navigation channel and complex, food-producing and foraging areas on the channel margins, namely shallow side channels and sandbar complexes. A favored hypothesis, the interception hypothesis, for recruitment failure of pallid sturgeon is that drifting larvae are not able to exit the highly engineered navigation channel, and therefore starve. We present work exploring measures of hydraulic connectivity between the navigation channel and channel margins using multiple data-collection protocols with acoustic Doppler current profilers (ADCPs). As ADCP datasets alone often do not have high enough spatial resolution to characterize interception and connectivity sufficiently at the scale of drifting sturgeon larvae, they are often supplemented with physical and empirical models. Using boat-mounted ADCPs, we collected 3-dimensional current velocities with a variety of driving techniques (specifically, regularly spaced transects, reciprocal transects, and irregular patterns) around areas of potential larval interception. We then used toolkits based in Python to interpolate 3-dimensional velocity fields at spatial scales finer than the original measurements, and visualized resultant velocity vectors and flowlines in the software package Paraview. Using these visualizations, we investigated the necessary resolution of field measurements required to model connectivity with channel margin areas on large, highly engineered river ecosystems such as the Missouri River. We anticipate that results from this work will be used to help inform models of larval interception under current conditions. Furthermore, results from this work will be useful in developing monitoring strategies to evaluate the restoration of channel complexity to support ecological functions.

Emirates Mars Ultraviolet Spectrometer (EMUS) Overview from the Emirates Mars Mission

NASA Astrophysics Data System (ADS)

Lootah, F. H.; Almatroushi, H. R.; AlMheiri, S.; Holsclaw, G.; Deighan, J.; Chaffin, M.; Reed, H.; Lillis, R. J.; Fillingim, M. O.; England, S.

2017-12-01

The Emirates Mars Ultraviolet Spectrometer (EMUS) instrument is one of three science instruments on board the "Hope Probe" of the Emirates Mars Mission (EMM). EMM is a United Arab Emirates' (UAE) mission to Mars, launching in 2020, to explore the global dynamics of the Martian atmosphere, while sampling on both diurnal and seasonal timescales. The EMUS instrument is a far-ultraviolet imaging spectrograph that measures emissions in the spectral range 100-170 nm. Using a combination of its one-dimensional imaging and spacecraft motion, it will build up two-dimensional far-ultraviolet images of the Martian disk and near-space environment at several important wavelengths: the Lyman beta atomic hydrogen emission (102.6 nm), the Lyman alpha atomic hydrogen emission (121.6 nm), two atomic oxygen emissions (130.4 nm and 135.6 nm), and the carbon monoxide fourth positive group band emission (140 nm-170 nm). Radiances at these wavelengths will be used to derive the column abundance of atomic oxygen, and carbon monoxide in the Martian thermosphere, and the density of atomic oxygen and atomic hydrogen in the Martian exosphere both with spatial and sub-seasonal variability. The EMUS instrument consists of a single telescope mirror feeding a Rowland circle imaging spectrograph with selectable spectral resolution (1.3 nm, 1.8 nm, or 5 nm), and a photon-counting and locating detector (provided by the Space Sciences Laboratory at the University of California, Berkeley). The EMUS spatial resolution of less than 300 km on the disk is sufficient to characterize spatial variability in the Martian thermosphere (100-200 km altitude) and exosphere (>200 km altitude). The instrument is jointly developed by the Laboratory for Atmospheric and Space Physics (LASP) at the University of Colorado Boulder and Mohammed Bin Rashid Space Centre (MBRSC) in Dubai, UAE.

Super resolution imaging of HER2 gene amplification

NASA Astrophysics Data System (ADS)

Okada, Masaya; Kubo, Takuya; Masumoto, Kanako; Iwanaga, Shigeki

2016-02-01

HER2 positive breast cancer is currently examined by counting HER2 genes using fluorescence in situ hybridization (FISH)-stained breast carcinoma samples. In this research, two-dimensional super resolution fluorescence microscopy based on stochastic optical reconstruction microscopy (STORM), with a spatial resolution of approximately 20 nm in the lateral direction, was used to more precisely distinguish and count HER2 genes in a FISH-stained tissue section. Furthermore, by introducing double-helix point spread function (DH-PSF), an optical phase modulation technique, to super resolution microscopy, three-dimensional images were obtained of HER2 in a breast carcinoma sample approximately 4 μm thick.

Finite-dimensional Liouville integrable Hamiltonian systems generated from Lax pairs of a bi-Hamiltonian soliton hierarchy by symmetry constraints

NASA Astrophysics Data System (ADS)

Manukure, Solomon

2018-04-01

We construct finite-dimensional Hamiltonian systems by means of symmetry constraints from the Lax pairs and adjoint Lax pairs of a bi-Hamiltonian hierarchy of soliton equations associated with the 3-dimensional special linear Lie algebra, and discuss the Liouville integrability of these systems based on the existence of sufficiently many integrals of motion.

Hyperspectral Super-Resolution of Locally Low Rank Images From Complementary Multisource Data.

PubMed

Veganzones, Miguel A; Simoes, Miguel; Licciardi, Giorgio; Yokoya, Naoto; Bioucas-Dias, Jose M; Chanussot, Jocelyn

2016-01-01

Remote sensing hyperspectral images (HSIs) are quite often low rank, in the sense that the data belong to a low dimensional subspace/manifold. This has been recently exploited for the fusion of low spatial resolution HSI with high spatial resolution multispectral images in order to obtain super-resolution HSI. Most approaches adopt an unmixing or a matrix factorization perspective. The derived methods have led to state-of-the-art results when the spectral information lies in a low-dimensional subspace/manifold. However, if the subspace/manifold dimensionality spanned by the complete data set is large, i.e., larger than the number of multispectral bands, the performance of these methods mainly decreases because the underlying sparse regression problem is severely ill-posed. In this paper, we propose a local approach to cope with this difficulty. Fundamentally, we exploit the fact that real world HSIs are locally low rank, that is, pixels acquired from a given spatial neighborhood span a very low-dimensional subspace/manifold, i.e., lower or equal than the number of multispectral bands. Thus, we propose to partition the image into patches and solve the data fusion problem independently for each patch. This way, in each patch the subspace/manifold dimensionality is low enough, such that the problem is not ill-posed anymore. We propose two alternative approaches to define the hyperspectral super-resolution through local dictionary learning using endmember induction algorithms. We also explore two alternatives to define the local regions, using sliding windows and binary partition trees. The effectiveness of the proposed approaches is illustrated with synthetic and semi real data.

Multiframe super resolution reconstruction method based on light field angular images

NASA Astrophysics Data System (ADS)

Zhou, Shubo; Yuan, Yan; Su, Lijuan; Ding, Xiaomin; Wang, Jichao

2017-12-01

The plenoptic camera can directly obtain 4-dimensional light field information from a 2-dimensional sensor. However, based on the sampling theorem, the spatial resolution is greatly limited by the microlenses. In this paper, we present a method of reconstructing high-resolution images from the angular images. First, the ray tracing method is used to model the telecentric-based light field imaging process. Then, we analyze the subpixel shifts between the angular images extracted from the defocused light field data and the blur in the angular images. According to the analysis above, we construct the observation model from the ideal high-resolution image to the angular images. Applying the regularized super resolution method, we can obtain the super resolution result with a magnification ratio of 8. The results demonstrate the effectiveness of the proposed observation model.

Towards a microchannel-based X-ray detector with two-dimensional spatial and time resolution and high dynamic range

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adams, Bernhard W.; Mane, Anil U.; Elam, Jeffrey W.

X-ray detectors that combine two-dimensional spatial resolution with a high time resolution are needed in numerous applications of synchrotron radiation. Most detectors with this combination of capabilities are based on semiconductor technology and are therefore limited in size. Furthermore, the time resolution is often realised through rapid time-gating of the acquisition, followed by a slower readout. Here, a detector technology is realised based on relatively inexpensive microchannel plates that uses GHz waveform sampling for a millimeter-scale spatial resolution and better than 100 ps time resolution. The technology is capable of continuous streaming of time- and location-tagged events at rates greatermore » than 10 7events per cm 2. Time-gating can be used for improved dynamic range.« less

A Three-Dimensional Target Depth-Resolution Method with a Single-Vector Sensor

PubMed Central

Zhao, Anbang; Bi, Xuejie; Hui, Juan; Zeng, Caigao; Ma, Lin

2018-01-01

This paper mainly studies and verifies the target number category-resolution method in multi-target cases and the target depth-resolution method of aerial targets. Firstly, target depth resolution is performed by using the sign distribution of the reactive component of the vertical complex acoustic intensity; the target category and the number resolution in multi-target cases is realized with a combination of the bearing-time recording information; and the corresponding simulation verification is carried out. The algorithm proposed in this paper can distinguish between the single-target multi-line spectrum case and the multi-target multi-line spectrum case. This paper presents an improved azimuth-estimation method for multi-target cases, which makes the estimation results more accurate. Using the Monte Carlo simulation, the feasibility of the proposed target number and category-resolution algorithm in multi-target cases is verified. In addition, by studying the field characteristics of the aerial and surface targets, the simulation results verify that there is only amplitude difference between the aerial target field and the surface target field under the same environmental parameters, and an aerial target can be treated as a special case of a surface target; the aerial target category resolution can then be realized based on the sign distribution of the reactive component of the vertical acoustic intensity so as to realize three-dimensional target depth resolution. By processing data from a sea experiment, the feasibility of the proposed aerial target three-dimensional depth-resolution algorithm is verified. PMID:29649173

A Three-Dimensional Target Depth-Resolution Method with a Single-Vector Sensor.

PubMed

Zhao, Anbang; Bi, Xuejie; Hui, Juan; Zeng, Caigao; Ma, Lin

2018-04-12

This paper mainly studies and verifies the target number category-resolution method in multi-target cases and the target depth-resolution method of aerial targets. Firstly, target depth resolution is performed by using the sign distribution of the reactive component of the vertical complex acoustic intensity; the target category and the number resolution in multi-target cases is realized with a combination of the bearing-time recording information; and the corresponding simulation verification is carried out. The algorithm proposed in this paper can distinguish between the single-target multi-line spectrum case and the multi-target multi-line spectrum case. This paper presents an improved azimuth-estimation method for multi-target cases, which makes the estimation results more accurate. Using the Monte Carlo simulation, the feasibility of the proposed target number and category-resolution algorithm in multi-target cases is verified. In addition, by studying the field characteristics of the aerial and surface targets, the simulation results verify that there is only amplitude difference between the aerial target field and the surface target field under the same environmental parameters, and an aerial target can be treated as a special case of a surface target; the aerial target category resolution can then be realized based on the sign distribution of the reactive component of the vertical acoustic intensity so as to realize three-dimensional target depth resolution. By processing data from a sea experiment, the feasibility of the proposed aerial target three-dimensional depth-resolution algorithm is verified.

Spider-web inspired multi-resolution graphene tactile sensor.

PubMed

Liu, Lu; Huang, Yu; Li, Fengyu; Ma, Ying; Li, Wenbo; Su, Meng; Qian, Xin; Ren, Wanjie; Tang, Kanglai; Song, Yanlin

2018-05-08

Multi-dimensional accurate response and smooth signal transmission are critical challenges in the advancement of multi-resolution recognition and complex environment analysis. Inspired by the structure-activity relationship between discrepant microstructures of the spiral and radial threads in a spider web, we designed and printed graphene with porous and densely-packed microstructures to integrate into a multi-resolution graphene tactile sensor. The three-dimensional (3D) porous graphene structure performs multi-dimensional deformation responses. The laminar densely-packed graphene structure contributes excellent conductivity with flexible stability. The spider-web inspired printed pattern inherits orientational and locational kinesis tracking. The multi-structure construction with homo-graphene material can integrate discrepant electronic properties with remarkable flexibility, which will attract enormous attention for electronic skin, wearable devices and human-machine interactions.

Whole-cell imaging of the budding yeast Saccharomyces cerevisiae by high-voltage scanning transmission electron tomography.

PubMed

Murata, Kazuyoshi; Esaki, Masatoshi; Ogura, Teru; Arai, Shigeo; Yamamoto, Yuta; Tanaka, Nobuo

2014-11-01

Electron tomography using a high-voltage electron microscope (HVEM) provides three-dimensional information about cellular components in sections thicker than 1 μm, although in bright-field mode image degradation caused by multiple inelastic scattering of transmitted electrons limit the attainable resolution. Scanning transmission electron microscopy (STEM) is believed to give enhanced contrast and resolution compared to conventional transmission electron microscopy (CTEM). Samples up to 1 μm in thickness have been analyzed with an intermediate-voltage electron microscope because inelastic scattering is not a critical limitation, and probe broadening can be minimized. Here, we employed STEM at 1 MeV high-voltage to extend the useful specimen thickness for electron tomography, which we demonstrate by a seamless tomographic reconstruction of a whole, budding Saccharomyces cerevisiae yeast cell, which is ~3 μm in thickness. High-voltage STEM tomography, especially in the bright-field mode, demonstrated sufficiently enhanced contrast and intensity, compared to CTEM tomography, to permit segmentation of major organelles in the whole cell. STEM imaging also reduced specimen shrinkage during tilt-series acquisition. The fidelity of structural preservation was limited by cytoplasmic extraction, and the spatial resolution was limited by the relatively large convergence angle of the scanning probe. However, the new technique has potential to solve longstanding problems of image blurring in biological specimens beyond 1 μm in thickness, and may facilitate new research in cellular structural biology. Copyright © 2014 Elsevier B.V. All rights reserved.

GRID: a high-resolution protein structure refinement algorithm.

PubMed

Chitsaz, Mohsen; Mayo, Stephen L

2013-03-05

The energy-based refinement of protein structures generated by fold prediction algorithms to atomic-level accuracy remains a major challenge in structural biology. Energy-based refinement is mainly dependent on two components: (1) sufficiently accurate force fields, and (2) efficient conformational space search algorithms. Focusing on the latter, we developed a high-resolution refinement algorithm called GRID. It takes a three-dimensional protein structure as input and, using an all-atom force field, attempts to improve the energy of the structure by systematically perturbing backbone dihedrals and side-chain rotamer conformations. We compare GRID to Backrub, a stochastic algorithm that has been shown to predict a significant fraction of the conformational changes that occur with point mutations. We applied GRID and Backrub to 10 high-resolution (≤ 2.8 Å) crystal structures from the Protein Data Bank and measured the energy improvements obtained and the computation times required to achieve them. GRID resulted in energy improvements that were significantly better than those attained by Backrub while expending about the same amount of computational resources. GRID resulted in relaxed structures that had slightly higher backbone RMSDs compared to Backrub relative to the starting crystal structures. The average RMSD was 0.25 ± 0.02 Å for GRID versus 0.14 ± 0.04 Å for Backrub. These relatively minor deviations indicate that both algorithms generate structures that retain their original topologies, as expected given the nature of the algorithms. Copyright © 2012 Wiley Periodicals, Inc.

Clinical applications of three-dimensional tortuosity metrics

NASA Astrophysics Data System (ADS)

Dougherty, Geoff; Johnson, Michael J.

2007-03-01

The measurement of abnormal vascular tortuosity is important in the diagnosis of many diseases. Metrics based on three-dimensional (3-D) curvature, using approximate polynomial spline-fitting to "data balls" centered along the mid-line of the vessel, minimize digitization errors and give tortuosity values largely independent of the resolution of the imaging system. In order to establish their clinical validity we applied them to a number of clinical vascular systems, using both 2-D (standard angiograms and retinal images) and 3-D datasets (from computed tomography angiography (CTA) and magnetic resonance angiography (MRA)). Using the abdominal aortograms we found that the metrics correlated well with the ranking of an expert panel of three vascular surgeons. Both the mean curvature and the root-mean square curvature provided good discrimination between vessels of different tortuosity: and using a data ball size of one-quarter of the local vessel radius in the spline fitting gave consistent results. Tortuous retinal vessels resulting from retinitis or diabetes, but not from vasculitis, could be distinguished from normal vessels. Tortuosity values based on 3-D data sets gave higher values than their 2-D projections, and could easily be implemented in automatic measurement. They produced values sufficiently discriminating to assess the relative utility of arteries for endoluminal repair of aneurysms.

Two-dimensional imaging in a lightweight portable MRI scanner without gradient coils.

PubMed

Cooley, Clarissa Zimmerman; Stockmann, Jason P; Armstrong, Brandon D; Sarracanie, Mathieu; Lev, Michael H; Rosen, Matthew S; Wald, Lawrence L

2015-02-01

As the premiere modality for brain imaging, MRI could find wider applicability if lightweight, portable systems were available for siting in unconventional locations such as intensive care units, physician offices, surgical suites, ambulances, emergency rooms, sports facilities, or rural healthcare sites. We construct and validate a truly portable (<100 kg) and silent proof-of-concept MRI scanner which replaces conventional gradient encoding with a rotating lightweight cryogen-free, low-field magnet. When rotated about the object, the inhomogeneous field pattern is used as a rotating spatial encoding magnetic field (rSEM) to create generalized projections which encode the iteratively reconstructed two-dimensional (2D) image. Multiple receive channels are used to disambiguate the nonbijective encoding field. The system is validated with experimental images of 2D test phantoms. Similar to other nonlinear field encoding schemes, the spatial resolution is position dependent with blurring in the center, but is shown to be likely sufficient for many medical applications. The presented MRI scanner demonstrates the potential for portability by simultaneously relaxing the magnet homogeneity criteria and eliminating the gradient coil. This new architecture and encoding scheme shows convincing proof of concept images that are expected to be further improved with refinement of the calibration and methodology. © 2014 Wiley Periodicals, Inc.

A three-dimensional ParF meshwork assembles through the nucleoid to mediate plasmid segregation.

PubMed

McLeod, Brett N; Allison-Gamble, Gina E; Barge, Madhuri T; Tonthat, Nam K; Schumacher, Maria A; Hayes, Finbarr; Barillà, Daniela

2017-04-07

Genome segregation is a fundamental step in the life cycle of every cell. Most bacteria rely on dedicated DNA partition proteins to actively segregate chromosomes and low copy-number plasmids. Here, by employing super resolution microscopy, we establish that the ParF DNA partition protein of the ParA family assembles into a three-dimensional meshwork that uses the nucleoid as a scaffold and periodically shuttles between its poles. Whereas ParF specifies the territory for plasmid trafficking, the ParG partner protein dictates the tempo of ParF assembly cycles and plasmid segregation events by stimulating ParF adenosine triphosphate hydrolysis. Mutants in which this ParG temporal regulation is ablated show partition deficient phenotypes as a result of either altered ParF structure or dynamics and indicate that ParF nucleoid localization and dynamic relocation, although necessary, are not sufficient per se to ensure plasmid segregation. We propose a Venus flytrap model that merges the concepts of ParA polymerization and gradient formation and speculate that a transient, dynamic network of intersecting polymers that branches into the nucleoid interior is a widespread mechanism to distribute sizeable cargos within prokaryotic cells. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

A Medipix quantum area detector allows rotation electron diffraction data collection from submicrometre three-dimensional protein crystals

PubMed Central

Nederlof, Igor; van Genderen, Eric; Li, Yao-Wang; Abrahams, Jan Pieter

2013-01-01

When protein crystals are submicrometre-sized, X-ray radiation damage precludes conventional diffraction data collection. For crystals that are of the order of 100 nm in size, at best only single-shot diffraction patterns can be collected and rotation data collection has not been possible, irrespective of the diffraction technique used. Here, it is shown that at a very low electron dose (at most 0.1 e− Å−2), a Medipix2 quantum area detector is sufficiently sensitive to allow the collection of a 30-frame rotation series of 200 keV electron-diffraction data from a single ∼100 nm thick protein crystal. A highly parallel 200 keV electron beam (λ = 0.025 Å) allowed observation of the curvature of the Ewald sphere at low resolution, indicating a combined mosaic spread/beam divergence of at most 0.4°. This result shows that volumes of crystal with low mosaicity can be pinpointed in electron diffraction. It is also shown that strategies and data-analysis software (MOSFLM and SCALA) from X-ray protein crystallography can be used in principle for analysing electron-diffraction data from three-dimensional nanocrystals of proteins. PMID:23793148

Monitoring Marine Weather Systems Using Quikscat and TRMM Data

NASA Technical Reports Server (NTRS)

Liu, W.; Tang, W.; Datta, A.; Hsu, C.

1999-01-01

We do not understand nor are able to predict marine storms, particularly tropical cyclones, sufficiently well because ground-based measurements are sparse and operational numerical weather prediction models do not have sufficient spatial resolution nor accurate parameterization of the physics.

In vivo two-dimensional NMR correlation spectroscopy

NASA Astrophysics Data System (ADS)

Kraft, Robert A.

1999-10-01

The poor resolution of in-vivo one- dimensional nuclear magnetic resonance spectroscopy (NMR) has limited its clinical potential. Currently, only the large singlet methyl resonances arising from N-acetyl aspartate (NAA), choline, and creatine are quantitated in a clinical setting. Other metabolites such as myo- inositol, glutamine, glutamate, lactate, and γ- amino butyric acid (GABA) are of clinical interest but quantitation is difficult due to the overlapping resonances and limited spectral resolution. To improve the spectral resolution and distinguish between overlapping resonances, a series of two- dimensional chemical shift correlation spectroscopy experiments were developed for a 1.5 Tesla clinical imaging magnet. Two-dimensional methods are attractive for in vivo spectroscopy due to their ability to unravel overlapping resonances with the second dimension, simplifying the interpretation and quantitation of low field NMR spectra. Two-dimensional experiments acquired with mix-mode line shape negate the advantages of the second dimension. For this reason, a new experiment, REVOLT, was developed to achieve absorptive mode line shape in both dimensions. Absorptive mode experiments were compared to mixed mode experiments with respect to sensitivity, resolution, and water suppression. Detailed theoretical and experimental calculations of the optimum spin lock and radio frequency power deposition were performed. Two-dimensional spectra were acquired from human bone marrow and human brain tissue. The human brain tissue spectra clearly reveal correlations among the coupled spins of NAA, glutamine, glutamate, lactate, GABA, aspartate and myo-inositol obtained from a single experiment of 23 minutes from a volume of 59 mL. (Copies available exclusively from MIT Libraries, Rm. 14-0551, Cambridge, MA 02139-4307. Ph. 617-253-5668; Fax 617-253-1690.)

Three-dimensional nanometre localization of nanoparticles to enhance super-resolution microscopy

NASA Astrophysics Data System (ADS)

Bon, Pierre; Bourg, Nicolas; Lécart, Sandrine; Monneret, Serge; Fort, Emmanuel; Wenger, Jérôme; Lévêque-Fort, Sandrine

2015-07-01

Meeting the nanometre resolution promised by super-resolution microscopy techniques (pointillist: PALM, STORM, scanning: STED) requires stabilizing the sample drifts in real time during the whole acquisition process. Metal nanoparticles are excellent probes to track the lateral drifts as they provide crisp and photostable information. However, achieving nanometre axial super-localization is still a major challenge, as diffraction imposes large depths-of-fields. Here we demonstrate fast full three-dimensional nanometre super-localization of gold nanoparticles through simultaneous intensity and phase imaging with a wavefront-sensing camera based on quadriwave lateral shearing interferometry. We show how to combine the intensity and phase information to provide the key to the third axial dimension. Presently, we demonstrate even in the occurrence of large three-dimensional fluctuations of several microns, unprecedented sub-nanometre localization accuracies down to 0.7 nm in lateral and 2.7 nm in axial directions at 50 frames per second. We demonstrate that nanoscale stabilization greatly enhances the image quality and resolution in direct stochastic optical reconstruction microscopy imaging.

Sub-micron resolution rf cavity beam position monitor system at the SACLA XFEL facility

NASA Astrophysics Data System (ADS)

Maesaka, H.; Ego, H.; Inoue, S.; Matsubara, S.; Ohshima, T.; Shintake, T.; Otake, Y.

2012-12-01

We have developed and constructed a C-band (4.760 GHz) rf cavity beam position monitor (RF-BPM) system for the XFEL facility at SPring-8, SACLA. The demanded position resolution of the RF-BPM is less than 1 μm, because an electron beam and x-rays must be overlapped within 4 μm precision in the undulator section for sufficient FEL interaction between the electrons and x-rays. In total, 57 RF-BPMs, including IQ demodulators and high-speed waveform digitizers for signal processing, were produced and installed into SACLA. We evaluated the position resolutions of 20 RF-BPMs in the undulator section by using a 7 GeV electron beam having a 0.1 nC bunch charge. The position resolution was measured to be less than 0.6 μm, which was sufficient for the XFEL lasing in the wavelength region of 0.1 nm, or shorter.

Formation of Spiral-Arm Spurs and Bound Clouds in Vertically Stratified Galactic Gas Disks

NASA Astrophysics Data System (ADS)

Kim, Woong-Tae; Ostriker, Eve C.

2006-07-01

We investigate the growth of spiral-arm substructure in vertically stratified, self-gravitating, galactic gas disks, using local numerical MHD simulations. Our new models extend our previous two-dimensional studies, which showed that a magnetized spiral shock in a thin disk can undergo magneto-Jeans instability (MJI), resulting in regularly spaced interarm spur structures and massive gravitationally bound fragments. Similar spur (or ``feather'') features have recently been seen in high-resolution observations of several galaxies. Here we consider two sets of numerical models: two-dimensional simulations that use a ``thick-disk'' gravitational kernel, and three-dimensional simulations with explicit vertical stratification. Both models adopt an isothermal equation of state with cs=7 km s-1. When disks are sufficiently magnetized and self-gravitating, the result in both sorts of models is the growth of spiral-arm substructure similar to that in our previous razor-thin models. Reduced self-gravity due to nonzero disk thickness increases the spur spacing to ~10 times the Jeans length at the arm peak. Bound clouds that form from spur fragmentation have masses ~(1-3)×107 Msolar each, similar to the largest observed GMCs. The mass-to-flux ratios and specific angular momenta of the bound condensations are lower than large-scale galactic values, as is true for observed GMCs. We find that unmagnetized or weakly magnetized two-dimensional models are unstable to the ``wiggle instability'' previously identified by Wada & Koda. However, our fully three-dimensional models do not show this effect. Nonsteady motions and strong vertical shear prevent coherent vortical structures from forming, evidently suppressing the wiggle instability. We also find no clear traces of Parker instability in the nonlinear spiral arm substructures that emerge, although conceivably Parker modes may help seed the MJI at early stages since azimuthal wavelengths are similar.

In-line three-dimensional holography of nanocrystalline objects at atomic resolution

PubMed Central

Chen, F.-R.; Van Dyck, D.; Kisielowski, C.

2016-01-01

Resolution and sensitivity of the latest generation aberration-corrected transmission electron microscopes allow the vast majority of single atoms to be imaged with sub-Ångstrom resolution and their locations determined in an image plane with a precision that exceeds the 1.9-pm wavelength of 300 kV electrons. Such unprecedented performance allows expansion of electron microscopic investigations with atomic resolution into the third dimension. Here we report a general tomographic method to recover the three-dimensional shape of a crystalline particle from high-resolution images of a single projection without the need for sample rotation. The method is compatible with low dose rate electron microscopy, which improves on signal quality, while minimizing electron beam-induced structure modifications even for small particles or surfaces. We apply it to germanium, gold and magnesium oxide particles, and achieve a depth resolution of 1–2 Å, which is smaller than inter-atomic distances. PMID:26887849

Ultrasound Backscatter Microscopy for Imaging of Oral Carcinoma

PubMed Central

Lam, Matthew; Chaudhari, Abhijit J.; Sun, Yang; Zhou, Feifei; Dobbie, Allison; Gandour-Edwards, Regina F.; Tinling, Steve P.; Farwell, D. Gregory; Monsky, Wayne L.; Shung, K. Kirk; Marcu, Laura

2013-01-01

Objectives Ultrasound backscatter microscopy (UBM), or ultrasound biomicroscopy, is a noninvasive, label-free, and ionizing radiation–free technique allowing high-resolution 3-dimensional structural imaging. The goal of this study was to evaluate UBM for resolving anatomic features associated with squamous cell carcinoma of the oral cavity. Methods The study was conducted in a hamster buccal pouch model. A carcinogen was topically applied to cheeks of 14 golden Syrian hamsters. Six additional hamsters served as healthy controls. A high-frequency (41 MHz, 6-mm focal depth, lateral and axial resolutions of 65 and 37 μm, respectively) UBM system was used for scanning the oral cavity after 14 weeks of carcinogen application. Histologic analyses were conducted on scanned regions. Results The histologic structure of buccal tissue and microvasculature networks could be visualized from the UBM images. Epithelial and mucosal hypertrophy and neoplastic changes were identified in animals subjected to the carcinogen. In animals with invasive squamous cell carcinoma, lesion development and destruction of the structural integrity of tissue layers were noted. Conclusions In this pilot study, UBM generated sufficient contrast for morphologic features associated with oral carcinoma compared to healthy tissue. This modality may present a practical technique for detection of oral neoplasms that is potentially translatable to humans. PMID:24065260

Design of a superconducting volume coil for magnetic resonance microscopy of the mouse brain

NASA Astrophysics Data System (ADS)

Nouls, John C.; Izenson, Michael G.; Greeley, Harold P.; Johnson, G. Allan

2008-04-01

We present the design process of a superconducting volume coil for magnetic resonance microscopy of the mouse brain at 9.4 T. The yttrium barium copper oxide coil has been designed through an iterative process of three-dimensional finite-element simulations and validation against room temperature copper coils. Compared to previous designs, the Helmholtz pair provides substantially higher B1 homogeneity over an extended volume of interest sufficiently large to image biologically relevant specimens. A custom-built cryogenic cooling system maintains the superconducting probe at 60 ± 0.1 K. Specimen loading and probe retuning can be carried out interactively with the coil at operating temperature, enabling much higher through-put. The operation of the probe is a routine, consistent procedure. Signal-to-noise ratio in a mouse brain increased by a factor ranging from 1.1 to 2.9 as compared to a room-temperature solenoid coil optimized for mouse brain microscopy. We demonstrate images encoded at 10 × 10 × 20 μm for an entire mouse brain specimen with signal-to-noise ratio of 18 and a total acquisition time of 16.5 h, revealing neuroanatomy unseen at lower resolution. Phantom measurements show an effective spatial resolution better than 20 μm.

On Galactic Density Modeling in the Presence of Dust Extinction

NASA Astrophysics Data System (ADS)

Bovy, Jo; Rix, Hans-Walter; Green, Gregory M.; Schlafly, Edward F.; Finkbeiner, Douglas P.

2016-02-01

Inferences about the spatial density or phase-space structure of stellar populations in the Milky Way require a precise determination of the effective survey volume. The volume observed by surveys such as Gaia or near-infrared spectroscopic surveys, which have good coverage of the Galactic midplane region, is highly complex because of the abundant small-scale structure in the three-dimensional interstellar dust extinction. We introduce a novel framework for analyzing the importance of small-scale structure in the extinction. This formalism demonstrates that the spatially complex effect of extinction on the selection function of a pencil-beam or contiguous sky survey is equivalent to a low-pass filtering of the extinction-affected selection function with the smooth density field. We find that the angular resolution of current 3D extinction maps is sufficient for analyzing Gaia sub-samples of millions of stars. However, the current distance resolution is inadequate and needs to be improved by an order of magnitude, especially in the inner Galaxy. We also present a practical and efficient method for properly taking the effect of extinction into account in analyses of Galactic structure through an effective selection function. We illustrate its use with the selection function of red-clump stars in APOGEE using and comparing a variety of current 3D extinction maps.

Modeling the periodic stratification and gravitational circulation in San Francisco Bay, California

USGS Publications Warehouse

Cheng, Ralph T.; Casulli, Vincenzo

1996-01-01

A high resolution, three-dimensional (3-D) hydrodynamic numerical model is applied to San Francisco Bay, California to simulate the periodic tidal stratification caused by tidal straining and stirring and their long-term effects on gravitational circulation. The numerical model is formulated using fixed levels in the vertical and uniform computational mesh on horizontal planes. The governing conservation equations, the 3-D shallow water equations, are solved by a semi-implicit finite-difference scheme. Numerical simulations for estuarine flows in San Francisco Bay have been performed to reproduce the hydrodynamic properties of tides, tidal and residual currents, and salt transport. All simulations were carried out to cover at least 30 days, so that the spring-neap variance in the model results could be analyzed. High grid resolution used in the model permits the use of a simple turbulence closure scheme which has been shown to be sufficient to reproduce the tidal cyclic stratification and well-mixed conditions in the water column. Low-pass filtered 3-D time-series reveals the classic estuarine gravitational circulation with a surface layer flowing down-estuary and an up-estuary flow near the bottom. The intensity of the gravitational circulation depends upon the amount of freshwater inflow, the degree of stratification, and spring-neap tidal variations.

Design of a superconducting volume coil for magnetic resonance microscopy of the mouse brain.

PubMed

Nouls, John C; Izenson, Michael G; Greeley, Harold P; Johnson, G Allan

2008-04-01

We present the design process of a superconducting volume coil for magnetic resonance microscopy of the mouse brain at 9.4T. The yttrium barium copper oxide coil has been designed through an iterative process of three-dimensional finite-element simulations and validation against room temperature copper coils. Compared to previous designs, the Helmholtz pair provides substantially higher B(1) homogeneity over an extended volume of interest sufficiently large to image biologically relevant specimens. A custom-built cryogenic cooling system maintains the superconducting probe at 60+/-0.1K. Specimen loading and probe retuning can be carried out interactively with the coil at operating temperature, enabling much higher through-put. The operation of the probe is a routine, consistent procedure. Signal-to-noise ratio in a mouse brain increased by a factor ranging from 1.1 to 2.9 as compared to a room-temperature solenoid coil optimized for mouse brain microscopy. We demonstrate images encoded at 10x10x20mum for an entire mouse brain specimen with signal-to-noise ratio of 18 and a total acquisition time of 16.5h, revealing neuroanatomy unseen at lower resolution. Phantom measurements show an effective spatial resolution better than 20mum.

Dimensional analysis of acoustically propagated signals

NASA Technical Reports Server (NTRS)

Hansen, Scott D.; Thomson, Dennis W.

1993-01-01

Traditionally, long term measurements of atmospherically propagated sound signals have consisted of time series of multiminute averages. Only recently have continuous measurements with temporal resolution corresponding to turbulent time scales been available. With modern digital data acquisition systems we now have the capability to simultaneously record both acoustical and meteorological parameters with sufficient temporal resolution to allow us to examine in detail relationships between fluctuating sound and the meteorological variables, particularly wind and temperature, which locally determine the acoustic refractive index. The atmospheric acoustic propagation medium can be treated as a nonlinear dynamical system, a kind of signal processor whose innards depend on thermodynamic and turbulent processes in the atmosphere. The atmosphere is an inherently nonlinear dynamical system. In fact one simple model of atmospheric convection, the Lorenz system, may well be the most widely studied of all dynamical systems. In this paper we report some results of our having applied methods used to characterize nonlinear dynamical systems to study the characteristics of acoustical signals propagated through the atmosphere. For example, we investigate whether or not it is possible to parameterize signal fluctuations in terms of fractal dimensions. For time series one such parameter is the limit capacity dimension. Nicolis and Nicolis were among the first to use the kind of methods we have to study the properties of low dimension global attractors.

Atomic Force Microscopy in Imaging of Viruses and Virus-Infected Cells

PubMed Central

Kuznetsov, Yurii G.; McPherson, Alexander

2011-01-01

Summary: Atomic force microscopy (AFM) can visualize almost everything pertinent to structural virology and at resolutions that approach those for electron microscopy (EM). Membranes have been identified, RNA and DNA have been visualized, and large protein assemblies have been resolved into component substructures. Capsids of icosahedral viruses and the icosahedral capsids of enveloped viruses have been seen at high resolution, in some cases sufficiently high to deduce the arrangement of proteins in the capsomeres as well as the triangulation number (T). Viruses have been recorded budding from infected cells and suffering the consequences of a variety of stresses. Mutant viruses have been examined and phenotypes described. Unusual structural features have appeared, and the unexpectedly great amount of structural nonconformity within populations of particles has been documented. Samples may be imaged in air or in fluids (including culture medium or buffer), in situ on cell surfaces, or after histological procedures. AFM is nonintrusive and nondestructive, and it can be applied to soft biological samples, particularly when the tapping mode is employed. In principle, only a single cell or virion need be imaged to learn of its structure, though normally images of as many as is practical are collected. While lateral resolution, limited by the width of the cantilever tip, is a few nanometers, height resolution is exceptional, at approximately 0.5 nm. AFM produces three-dimensional, topological images that accurately depict the surface features of the virus or cell under study. The images resemble common light photographic images and require little interpretation. The structures of viruses observed by AFM are consistent with models derived by X-ray crystallography and cryo-EM. PMID:21646429

SOMAR-LES: A framework for multi-scale modeling of turbulent stratified oceanic flows

NASA Astrophysics Data System (ADS)

Chalamalla, Vamsi K.; Santilli, Edward; Scotti, Alberto; Jalali, Masoud; Sarkar, Sutanu

2017-12-01

A new multi-scale modeling technique, SOMAR-LES, is presented in this paper. Localized grid refinement gives SOMAR (the Stratified Ocean Model with Adaptive Resolution) access to small scales of the flow which are normally inaccessible to general circulation models (GCMs). SOMAR-LES drives a LES (Large Eddy Simulation) on SOMAR's finest grids, forced with large scale forcing from the coarser grids. Three-dimensional simulations of internal tide generation, propagation and scattering are performed to demonstrate this multi-scale modeling technique. In the case of internal tide generation at a two-dimensional bathymetry, SOMAR-LES is able to balance the baroclinic energy budget and accurately model turbulence losses at only 10% of the computational cost required by a non-adaptive solver running at SOMAR-LES's fine grid resolution. This relative cost is significantly reduced in situations with intermittent turbulence or where the location of the turbulence is not known a priori because SOMAR-LES does not require persistent, global, high resolution. To illustrate this point, we consider a three-dimensional bathymetry with grids adaptively refined along the tidally generated internal waves to capture remote mixing in regions of wave focusing. The computational cost in this case is found to be nearly 25 times smaller than that of a non-adaptive solver at comparable resolution. In the final test case, we consider the scattering of a mode-1 internal wave at an isolated two-dimensional and three-dimensional topography, and we compare the results with Legg (2014) numerical experiments. We find good agreement with theoretical estimates. SOMAR-LES is less dissipative than the closure scheme employed by Legg (2014) near the bathymetry. Depending on the flow configuration and resolution employed, a reduction of more than an order of magnitude in computational costs is expected, relative to traditional existing solvers.

A new Lagrangian method for three-dimensional steady supersonic flows

NASA Technical Reports Server (NTRS)

Loh, Ching-Yuen; Liou, Meng-Sing

1993-01-01

In this report, the new Lagrangian method introduced by Loh and Hui is extended for three-dimensional, steady supersonic flow computation. The derivation of the conservation form and the solution of the local Riemann solver using the Godunov and the high-resolution TVD (total variation diminished) scheme is presented. This new approach is accurate and robust, capable of handling complicated geometry and interactions between discontinuous waves. Test problems show that the extended Lagrangian method retains all the advantages of the two-dimensional method (e.g., crisp resolution of a slip-surface (contact discontinuity) and automatic grid generation). In this report, we also suggest a novel three dimensional Riemann problem in which interesting and intricate flow features are present.

High resolution three-dimensional magnetization mapping in Tokachidake Volcano using low altitude airborne magnetic survey data

NASA Astrophysics Data System (ADS)

Iwata, M.; Mogi, T.; Okuma, S.; Nakatsuka, T.

2016-12-01

Tokachidake Volcano, central Hokkaido, Japan erupted in 1926, 1962 and 1988-1989 in the 20th century from the central part. In recent years, expansions of the edifice of the volcano at shallow depth and increases of the volcanic smoke in the Taisho crater were observed (Meteorological Agency of Japan, 2014). Magnetic changes were observed at the 62-2 crater by repeated magnetic measurements in 2008-2009, implying a demagnetization beneath the crater (Hashimoto at al., 2010). Moreover, a very low resistivity part was found right under the 62-2 crater from an AMT survey (Yamaya et al., 2010). However, since the station numbers of the survey are limited, the area coverage is not sufficient. In this study, we have re-analyzed high-resolution aeromagnetic data to delineate the three-dimensional magnetic structure of the volcano to understand the nature of other craters.A low altitude airborne magnetic survey was conducted in 2014 mainly over the active areas of the volcano by the Ministry of Land, Infrastructure, Transport and Tourism to manage land slide risk in the volcano. The survey was flown at an altitude of 60 m above ground by a helicopter with a Cesium magnetometer in the towed-bird 30m below the helicopter. The low altitude survey enables us to delineate the detailed magnetic structure. We calculated magnetic anomaly distribution on a smooth surface assuming equivalent anomalies below the observation surface. Then the 3D magnetic imaging method (Nakatsuka and Okuma, 2014) was applied to the magnetic anomalies to reveal the three-dimensional magnetic structure.As a result, magnetization highs were seen beneath the Ground crater, Suribachi crater and Kitamuki crater. This implies that magmatic activity occurred in the past at these craters. These magma should have already solidified and acquired strong remanent magnetization. Relative magnetization lows were seen beneath the 62-2 crater and the Taisho crater where fumarolic activity is active. However a magnetization high was seen beneath the Nukkakushi crater where fumarolic activity and hydrothermal alteration had been observed on the ground. Further studies on this interesting distribution is necessary.

Dynamic-focusing microscope objective for optical coherence tomography

NASA Astrophysics Data System (ADS)

Murali, Supraja; Rolland, Jannick

2007-01-01

Optical Coherence Tomography (OCT) is a novel optical imaging technique that has assumed significant importance in bio-medical imaging in the last two decades because it is non-invasive and provides accurate, high resolution images of three dimensional cross-sections of body tissue, exceeding the capabilities of the current predominant imaging technique - ultrasound. In this paper, the application of high resolution OCT, known as optical coherence microscopy (OCM) is investigated for in vivo detection of abnormal skin pathology for the early diagnosis of cancer. A main challenge in OCM is maintaining invariant resolution throughout the sample. The technology presented is based on a dynamic focusing microscope imaging probe conceived for skin imaging and the detection of abnormalities in the epithelium. A novel method for dynamic focusing in the biological sample is presented using variable-focus lens technology to obtain three dimensional images with invariant resolution throughout the cross-section and depth of the sample is presented and discussed. A low coherence broadband source centered at near IR wavelengths is used to illuminate the sample. The design, analysis and predicted performance of the dynamic focusing microscope objective designed for dynamic three dimensional imaging at 5μm resolution for the chosen broadband spectrum is presented.

Three-dimensional characterization of the effective second-order nonlinearity in periodically poled crystals

NASA Astrophysics Data System (ADS)

Holmgren, Stefan J.; Pasiskevicius, Valdas; Wang, Shunhua; Laurell, Fredrik

2003-09-01

A novel technique for characterization of the second-order nonlinearity in nonlinear crystals is presented. It utilizes group-velocity walk-off between femtosecond pulses in type II SHG to achieve three-dimensional resolution of the nonlinearity. The longitudinal and transversal spatial resolution can be set independently. The technique is especially useful for characterizing quasi-phase-matched nonlinear crystals, and it is demonstrated in potassium titanyl phosphate.

A synchrotron radiation microtomography system for the analysis of trabecular bone samples.

PubMed

Salomé, M; Peyrin, F; Cloetens, P; Odet, C; Laval-Jeantet, A M; Baruchel, J; Spanne, P

1999-10-01

X-ray computed microtomography is particularly well suited for studying trabecular bone architecture, which requires three-dimensional (3-D) images with high spatial resolution. For this purpose, we describe a three-dimensional computed microtomography (microCT) system using synchrotron radiation, developed at ESRF. Since synchrotron radiation provides a monochromatic and high photon flux x-ray beam, it allows high resolution and a high signal-to-noise ratio imaging. The principle of the system is based on truly three-dimensional parallel tomographic acquisition. It uses a two-dimensional (2-D) CCD-based detector to record 2-D radiographs of the transmitted beam through the sample under different angles of view. The 3-D tomographic reconstruction, performed by an exact 3-D filtered backprojection algorithm, yields 3-D images with cubic voxels. The spatial resolution of the detector was experimentally measured. For the application to bone investigation, the voxel size was set to 6.65 microm, and the experimental spatial resolution was found to be 11 microm. The reconstructed linear attenuation coefficient was calibrated from hydroxyapatite phantoms. Image processing tools are being developed to extract structural parameters quantifying trabecular bone architecture from the 3-D microCT images. First results on human trabecular bone samples are presented.

High resolution three-dimensional robotic synthetic tracked aperture ultrasound imaging: feasibility study

NASA Astrophysics Data System (ADS)

Zhang, Haichong K.; Fang, Ting Yun; Finocchi, Rodolfo; Boctor, Emad M.

2017-03-01

Three dimensional (3D) ultrasound imaging is becoming a standard mode for medical ultrasound diagnoses. Conventional 3D ultrasound imaging is mostly scanned either by using a two dimensional matrix array or by motorizing a one dimensional array in the elevation direction. However, the former system is not widely assessable due to its cost, and the latter one has limited resolution and field-of-view in the elevation axis. Here, we propose a 3D ultrasound imaging system based on the synthetic tracked aperture approach, in which a robotic arm is used to provide accurate tracking and motion. While the ultrasound probe is moved by a robotic arm, each probe position is tracked and can be used to reconstruct a wider field-of-view as there are no physical barriers that restrict the elevational scanning. At the same time, synthetic aperture beamforming provides a better resolution in the elevation axis. To synthesize the elevational information, the single focal point is regarded as the virtual element, and forward and backward delay-andsum are applied to the radio-frequency (RF) data collected through the volume. The concept is experimentally validated using a general ultrasound phantom, and the elevational resolution improvement of 2.54 and 2.13 times was measured at the target depths of 20 mm and 110 mm, respectively.

Energy dispersive CdTe and CdZnTe detectors for spectral clinical CT and NDT applications

NASA Astrophysics Data System (ADS)

Barber, W. C.; Wessel, J. C.; Nygard, E.; Iwanczyk, J. S.

2015-06-01

We are developing room temperature compound semiconductor detectors for applications in energy-resolved high-flux single x-ray photon-counting spectral computed tomography (CT), including functional imaging with nanoparticle contrast agents for medical applications and non-destructive testing (NDT) for security applications. Energy-resolved photon-counting can provide reduced patient dose through optimal energy weighting for a particular imaging task in CT, functional contrast enhancement through spectroscopic imaging of metal nanoparticles in CT, and compositional analysis through multiple basis function material decomposition in CT and NDT. These applications produce high input count rates from an x-ray generator delivered to the detector. Therefore, in order to achieve energy-resolved single photon counting in these applications, a high output count rate (OCR) for an energy-dispersive detector must be achieved at the required spatial resolution and across the required dynamic range for the application. The required performance in terms of the OCR, spatial resolution, and dynamic range must be obtained with sufficient field of view (FOV) for the application thus requiring the tiling of pixel arrays and scanning techniques. Room temperature cadmium telluride (CdTe) and cadmium zinc telluride (CdZnTe) compound semiconductors, operating as direct conversion x-ray sensors, can provide the required speed when connected to application specific integrated circuits (ASICs) operating at fast peaking times with multiple fixed thresholds per pixel provided the sensors are designed for rapid signal formation across the x-ray energy ranges of the application at the required energy and spatial resolutions, and at a sufficiently high detective quantum efficiency (DQE). We have developed high-flux energy-resolved photon-counting x-ray imaging array sensors using pixellated CdTe and CdZnTe semiconductors optimized for clinical CT and security NDT. We have also fabricated high-flux ASICs with a two dimensional (2D) array of inputs for readout from the sensors. The sensors are guard ring free and have a 2D array of pixels and can be tiled in 2D while preserving pixel pitch. The 2D ASICs have four energy bins with a linear energy response across sufficient dynamic range for clinical CT and some NDT applications. The ASICs can also be tiled in 2D and are designed to fit within the active area of the sensors. We have measured several important performance parameters including: the output count rate (OCR) in excess of 20 million counts per second per square mm with a minimum loss of counts due to pulse pile-up, an energy resolution of 7 keV full width at half-maximum (FWHM) across the entire dynamic range, and a noise floor about 20 keV. This is achieved by directly interconnecting the ASIC inputs to the pixels of the CdZnTe sensors incurring very little input capacitance to the ASICs. We present measurements of the performance of the CdTe and CdZnTe sensors including the OCR, FWHM energy resolution, noise floor, as well as the temporal stability and uniformity under the rapidly varying high flux expected in CT and NDT applications.

Energy dispersive CdTe and CdZnTe detectors for spectral clinical CT and NDT applications

PubMed Central

Barber, W. C.; Wessel, J. C.; Nygard, E.; Iwanczyk, J. S.

2014-01-01

We are developing room temperature compound semiconductor detectors for applications in energy-resolved high-flux single x-ray photon-counting spectral computed tomography (CT), including functional imaging with nanoparticle contrast agents for medical applications and non destructive testing (NDT) for security applications. Energy-resolved photon-counting can provide reduced patient dose through optimal energy weighting for a particular imaging task in CT, functional contrast enhancement through spectroscopic imaging of metal nanoparticles in CT, and compositional analysis through multiple basis function material decomposition in CT and NDT. These applications produce high input count rates from an x-ray generator delivered to the detector. Therefore, in order to achieve energy-resolved single photon counting in these applications, a high output count rate (OCR) for an energy-dispersive detector must be achieved at the required spatial resolution and across the required dynamic range for the application. The required performance in terms of the OCR, spatial resolution, and dynamic range must be obtained with sufficient field of view (FOV) for the application thus requiring the tiling of pixel arrays and scanning techniques. Room temperature cadmium telluride (CdTe) and cadmium zinc telluride (CdZnTe) compound semiconductors, operating as direct conversion x-ray sensors, can provide the required speed when connected to application specific integrated circuits (ASICs) operating at fast peaking times with multiple fixed thresholds per pixel provided the sensors are designed for rapid signal formation across the x-ray energy ranges of the application at the required energy and spatial resolutions, and at a sufficiently high detective quantum efficiency (DQE). We have developed high-flux energy-resolved photon-counting x-ray imaging array sensors using pixellated CdTe and CdZnTe semiconductors optimized for clinical CT and security NDT. We have also fabricated high-flux ASICs with a two dimensional (2D) array of inputs for readout from the sensors. The sensors are guard ring free and have a 2D array of pixels and can be tiled in 2D while preserving pixel pitch. The 2D ASICs have four energy bins with a linear energy response across sufficient dynamic range for clinical CT and some NDT applications. The ASICs can also be tiled in 2D and are designed to fit within the active area of the sensors. We have measured several important performance parameters including; the output count rate (OCR) in excess of 20 million counts per second per square mm with a minimum loss of counts due to pulse pile-up, an energy resolution of 7 keV full width at half maximum (FWHM) across the entire dynamic range, and a noise floor about 20keV. This is achieved by directly interconnecting the ASIC inputs to the pixels of the CdZnTe sensors incurring very little input capacitance to the ASICs. We present measurements of the performance of the CdTe and CdZnTe sensors including the OCR, FWHM energy resolution, noise floor, as well as the temporal stability and uniformity under the rapidly varying high flux expected in CT and NDT applications. PMID:25937684

Energy dispersive CdTe and CdZnTe detectors for spectral clinical CT and NDT applications.

PubMed

Barber, W C; Wessel, J C; Nygard, E; Iwanczyk, J S

2015-06-01

We are developing room temperature compound semiconductor detectors for applications in energy-resolved high-flux single x-ray photon-counting spectral computed tomography (CT), including functional imaging with nanoparticle contrast agents for medical applications and non destructive testing (NDT) for security applications. Energy-resolved photon-counting can provide reduced patient dose through optimal energy weighting for a particular imaging task in CT, functional contrast enhancement through spectroscopic imaging of metal nanoparticles in CT, and compositional analysis through multiple basis function material decomposition in CT and NDT. These applications produce high input count rates from an x-ray generator delivered to the detector. Therefore, in order to achieve energy-resolved single photon counting in these applications, a high output count rate (OCR) for an energy-dispersive detector must be achieved at the required spatial resolution and across the required dynamic range for the application. The required performance in terms of the OCR, spatial resolution, and dynamic range must be obtained with sufficient field of view (FOV) for the application thus requiring the tiling of pixel arrays and scanning techniques. Room temperature cadmium telluride (CdTe) and cadmium zinc telluride (CdZnTe) compound semiconductors, operating as direct conversion x-ray sensors, can provide the required speed when connected to application specific integrated circuits (ASICs) operating at fast peaking times with multiple fixed thresholds per pixel provided the sensors are designed for rapid signal formation across the x-ray energy ranges of the application at the required energy and spatial resolutions, and at a sufficiently high detective quantum efficiency (DQE). We have developed high-flux energy-resolved photon-counting x-ray imaging array sensors using pixellated CdTe and CdZnTe semiconductors optimized for clinical CT and security NDT. We have also fabricated high-flux ASICs with a two dimensional (2D) array of inputs for readout from the sensors. The sensors are guard ring free and have a 2D array of pixels and can be tiled in 2D while preserving pixel pitch. The 2D ASICs have four energy bins with a linear energy response across sufficient dynamic range for clinical CT and some NDT applications. The ASICs can also be tiled in 2D and are designed to fit within the active area of the sensors. We have measured several important performance parameters including; the output count rate (OCR) in excess of 20 million counts per second per square mm with a minimum loss of counts due to pulse pile-up, an energy resolution of 7 keV full width at half maximum (FWHM) across the entire dynamic range, and a noise floor about 20keV. This is achieved by directly interconnecting the ASIC inputs to the pixels of the CdZnTe sensors incurring very little input capacitance to the ASICs. We present measurements of the performance of the CdTe and CdZnTe sensors including the OCR, FWHM energy resolution, noise floor, as well as the temporal stability and uniformity under the rapidly varying high flux expected in CT and NDT applications.

Enhanced spectral resolution by high-dimensional NMR using the filter diagonalization method and "hidden" dimensions.

PubMed

Meng, Xi; Nguyen, Bao D; Ridge, Clark; Shaka, A J

2009-01-01

High-dimensional (HD) NMR spectra have poorer digital resolution than low-dimensional (LD) spectra, for a fixed amount of experiment time. This has led to "reduced-dimensionality" strategies, in which several LD projections of the HD NMR spectrum are acquired, each with higher digital resolution; an approximate HD spectrum is then inferred by some means. We propose a strategy that moves in the opposite direction, by adding more time dimensions to increase the information content of the data set, even if only a very sparse time grid is used in each dimension. The full HD time-domain data can be analyzed by the filter diagonalization method (FDM), yielding very narrow resonances along all of the frequency axes, even those with sparse sampling. Integrating over the added dimensions of HD FDM NMR spectra reconstitutes LD spectra with enhanced resolution, often more quickly than direct acquisition of the LD spectrum with a larger number of grid points in each of the fewer dimensions. If the extra-dimensions do not appear in the final spectrum, and are used solely to boost information content, we propose the moniker hidden-dimension NMR. This work shows that HD peaks have unmistakable frequency signatures that can be detected as single HD objects by an appropriate algorithm, even though their patterns would be tricky for a human operator to visualize or recognize, and even if digital resolution in an HD FT spectrum is very coarse compared with natural line widths.

4Pi-confocal microscopy of live cells

NASA Astrophysics Data System (ADS)

Bahlmann, Karsten; Jakobs, Stefan; Hell, Stefan W.

2002-06-01

By coherently adding the spherical wavefronts of two opposing lenses, two-photon excitation 4Pi-confocal fluorescence microscopy has achieved three-dimensional imaging with an axial resolution 3-7 times better than confocal microscopy. So far this improvement was possible only in glycerol-mounted, fixed cells. Here we report 4Pi-confocal microscopy of watery objects and its application to the imaging of live cells. Water immersion 4Pi-confocal microscopy of membrane stained live Escherichia coli bacteria attains a 4.3 fold better axial resolution as compared to the best water immersion confocal microscope. The resolution enhancement results into a vastly improved three-dimensional representation of the bacteria. The first images of live biological samples with an all-directional resolution in the 190-280 nm range are presented here, thus establishing a new resolution benchmark in live cell microscopy.

High-resolution and high-throughput multichannel Fourier transform spectrometer with two-dimensional interferogram warping compensation

NASA Astrophysics Data System (ADS)

Watanabe, A.; Furukawa, H.

2018-04-01

The resolution of multichannel Fourier transform (McFT) spectroscopy is insufficient for many applications despite its extreme advantage of high throughput. We propose an improved configuration to realise both performance using a two-dimensional area sensor. For the spectral resolution, we obtained the interferogram of a larger optical path difference by shifting the area sensor without altering any optical components. The non-linear phase error of the interferometer was successfully corrected using a phase-compensation calculation. Warping compensation was also applied to realise a higher throughput to accumulate the signal between vertical pixels. Our approach significantly improved the resolution and signal-to-noise ratio by factors of 1.7 and 34, respectively. This high-resolution and high-sensitivity McFT spectrometer will be useful for detecting weak light signals such as those in non-invasive diagnosis.

Inverse regression-based uncertainty quantification algorithms for high-dimensional models: Theory and practice

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Weixuan; Lin, Guang; Li, Bing

2016-09-01

A well-known challenge in uncertainty quantification (UQ) is the "curse of dimensionality". However, many high-dimensional UQ problems are essentially low-dimensional, because the randomness of the quantity of interest (QoI) is caused only by uncertain parameters varying within a low-dimensional subspace, known as the sufficient dimension reduction (SDR) subspace. Motivated by this observation, we propose and demonstrate in this paper an inverse regression-based UQ approach (IRUQ) for high-dimensional problems. Specifically, we use an inverse regression procedure to estimate the SDR subspace and then convert the original problem to a low-dimensional one, which can be efficiently solved by building a response surface model such as a polynomial chaos expansion. The novelty and advantages of the proposed approach is seen in its computational efficiency and practicality. Comparing with Monte Carlo, the traditionally preferred approach for high-dimensional UQ, IRUQ with a comparable cost generally gives much more accurate solutions even for high-dimensional problems, and even when the dimension reduction is not exactly sufficient. Theoretically, IRUQ is proved to converge twice as fast as the approach it uses seeking the SDR subspace. For example, while a sliced inverse regression method converges to the SDR subspace at the rate ofmore » $$O(n^{-1/2})$$, the corresponding IRUQ converges at $$O(n^{-1})$$. IRUQ also provides several desired conveniences in practice. It is non-intrusive, requiring only a simulator to generate realizations of the QoI, and there is no need to compute the high-dimensional gradient of the QoI. Finally, error bars can be derived for the estimation results reported by IRUQ.« less

The Three Dimensional Flow Field at the Exit of an Axial-Flow Turbine Rotor

NASA Technical Reports Server (NTRS)

Lakshminarayana, B.; Ristic, D.; Chu, S.

1998-01-01

A systematic and comprehensive investigation was performed to provide detailed data on the three dimensional viscous flow phenomena downstream of a modem turbine rotor and to understand the flow physics such as origin, nature, development of wakes, secondary flow, and leakage flow. The experiment was carried out in the Axial Flow Turbine Research Facility (AFTRF) at Penn State, with velocity measurements taken with a 3-D LDV System. Two radial traverses at 1% and 10% of chord downstream of the rotor have been performed to identify the three-dimensional flow features at the exit of the rotor blade row. Sufficient spatial resolution was maintained to resolve blade wake, secondary flow, and tip leakage flow. The wake deficit is found to be substantial, especially at 1% of chord downstream of the rotor. At this location, negative axial velocity occurs near the tip, suggesting flow separation in the tip clearance region. Turbulence intensities peak in the wake region, and cross- correlations are mainly associated with the velocity gradient of the wake deficit. The radial velocities, both in the wake and in the endwall region, are found to be substantial. Two counter-rotating secondary flows are identified in the blade passage, with one occupying the half span close to the casino and the other occupying the half span close to the hub. The tip leakage flow is well restricted to 10% immersion from the blade tip. There are strong vorticity distributions associated with these secondary flows and tip leakage flow. The passage averaged data are in good agreement with design values.

Three-dimensional reconstruction of coronary stents in vivo based on motion compensated X-ray angiography

NASA Astrophysics Data System (ADS)

Schäfer, Dirk; Movassaghi, Babak; Grass, Michael; Schoonenberg, Gert; Florent, Raoul; Wink, Onno; Klein, Andrew J. P.; Chen, James Y.; Garcia, Joel; Messenger, John C.; Carroll, John D.

2007-03-01

The complete expansion of the stent during a percutaneous transluminal coronary angioplasty (PTCA) procedure is essential for treatment of a stenotic segment of a coronary artery. Inadequate expansion of the stent is a major predisposing factor to in-stent restenosis and acute thrombosis. Stents are positioned and deployed by fluoroscopic guidance. Although the current generation of stents are made of materials with some degree of radio-opacity to detect their location after deployment, proper stent expansion is hard to asses. In this work, we introduce a new method for the three-dimensional (3D) reconstruction of the coronary stents in-vivo utilizing two-dimensional projection images acquired during rotational angiography (RA). The acquisition protocol consist of a propeller rotation of the X-ray C-arm system of 180°, which ensures sufficient angular coverage for volume reconstruction. The angiographic projections were acquired at 30 frames per second resulting in 180 projections during a 7 second rotational run. The motion of the stent is estimated from the automatically tracked 2D coordinates of the markers on the balloon catheter. This information is used within a motion-compensated reconstruction algorithm. Therefore, projections from different cardiac phases and motion states can be used, resulting in improved signal-to-noise ratio of the stent. Results of 3D reconstructed coronary stents in vivo, with high spatial resolution are presented. The proposed method allows for a comprehensive and unique quantitative 3D assessment of stent expansion that rivals current X-ray and intravascular ultrasound techniques.

Real-time Assimilation of Altimeter Derived Synthetic Profiles Into a Global version of the Naval Research Laboratory's Coastal Ocean Model (NCOM)

NASA Astrophysics Data System (ADS)

Rhodes, R. C.; Barron, C. N.; Fox, D. N.; Smedstad, L. F.

2001-12-01

A global implementation of the Navy Coastal Ocean Model (NCOM), developed by the Naval Research Laboratory (NRL) at Stennis Space Center is currently running in real-time and is planned for transition to the Naval Oceanographic Office (NAVOCEANO) in 2002. The model encompasses the open ocean to 5 m depth on a curvilinear global model grid with 1/8 degree grid spacing at 45N, extending from 80 S to a complete arctic cap with grid singularities mapped into Canada and Russia. Vertically, the model employs 41 sigma-z levels with sigma in the upper-ocean and coastal regions and z in the deeper ocean. The Navy Operational Global Atmospheric Prediction System (NOGAPS) provides 6-hourly wind stresses and heat fluxes for forcing, while the operational Modular Ocean Data Assimilation System (MODAS) provides the background climatology and tools for data pre-processing. Operationally available sea surface temperature (SST) and altimetry (SSH) data are assimilated into the NAVOCEANO global 1/8 degree MODAS 2-D analysis and the 1/16 degree Navy Layered Ocean Model (NLOM) to provide analyses and forecasts of SSH and SST. The 2-D SSH and SST nowcast fields are used as input to the MODAS synthetic climatology database to yield three-dimensional fields of synthetic temperature and salinity for assimilation into global NCOM. The synthetic profiles are weighted higher at depth in the assimilation process to allow the numerical model to properly develop the mixed-layer structure driven by the real-time atmospheric forcing. Global NCOM nowcasts and forecasts provide a valuable resource for rapid response to the varied and often unpredictable operational requests for 3-dimensional fields of ocean temperature, salinity, and currents. In some cases, the resolution of the global product is sufficient for guidance. In cases requiring higher resolution, the global product offers a quick overview of local circulation and provides initial and boundary conditions for higher resolution coastal models that may be more specialized for a particular task or domain. Nowcast and forecast results are presented globally and in selected areas of interest and model results are compared with historical and concurrent observations and analyses.

Dual-dimensional microscopy: real-time in vivo three-dimensional observation method using high-resolution light-field microscopy and light-field display.

PubMed

Kim, Jonghyun; Moon, Seokil; Jeong, Youngmo; Jang, Changwon; Kim, Youngmin; Lee, Byoungho

2018-06-01

Here, we present dual-dimensional microscopy that captures both two-dimensional (2-D) and light-field images of an in-vivo sample simultaneously, synthesizes an upsampled light-field image in real time, and visualizes it with a computational light-field display system in real time. Compared with conventional light-field microscopy, the additional 2-D image greatly enhances the lateral resolution at the native object plane up to the diffraction limit and compensates for the image degradation at the native object plane. The whole process from capturing to displaying is done in real time with the parallel computation algorithm, which enables the observation of the sample's three-dimensional (3-D) movement and direct interaction with the in-vivo sample. We demonstrate a real-time 3-D interactive experiment with Caenorhabditis elegans. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Wavefront sensor-driven variable-geometry pupil for ground-based aperture synthesis imaging

NASA Astrophysics Data System (ADS)

Tyler, David W.

2000-07-01

I describe a variable-geometry pupil (VGP) to increase image resolution for ground-based near-IR and optical imaging. In this scheme, a curvature-type wavefront sensor provides an estimate of the wavefront curvature to the controller of a high-resolution spatial light modulator (SLM) or micro- electromechanical (MEM) mirror, positioned at an image of the telescope pupil. This optical element, the VGP, passes or reflects the incident beam only where the wavefront phase is sufficiently smooth, viz., where the curvature is sufficiently low. Using a computer simulation, I show the VGP can sharpen and smooth the long-exposure PSF and increase the OTF SNR for tilt-only and low-order AO systems, allowing higher resolution and more stable deconvolution with dimmer AO guidestars.

Complex behavior in chains of nonlinear oscillators.

PubMed

Alonso, Leandro M

2017-06-01

This article outlines sufficient conditions under which a one-dimensional chain of identical nonlinear oscillators can display complex spatio-temporal behavior. The units are described by phase equations and consist of excitable oscillators. The interactions are local and the network is poised to a critical state by balancing excitation and inhibition locally. The results presented here suggest that in networks composed of many oscillatory units with local interactions, excitability together with balanced interactions is sufficient to give rise to complex emergent features. For values of the parameters where complex behavior occurs, the system also displays a high-dimensional bifurcation where an exponentially large number of equilibria are borne in pairs out of multiple saddle-node bifurcations.

A consensus embedding approach for segmentation of high resolution in vivo prostate magnetic resonance imagery

NASA Astrophysics Data System (ADS)

Viswanath, Satish; Rosen, Mark; Madabhushi, Anant

2008-03-01

Current techniques for localization of prostatic adenocarcinoma (CaP) via blinded trans-rectal ultrasound biopsy are associated with a high false negative detection rate. While high resolution endorectal in vivo Magnetic Resonance (MR) prostate imaging has been shown to have improved contrast and resolution for CaP detection over ultrasound, similarity in intensity characteristics between benign and cancerous regions on MR images contribute to a high false positive detection rate. In this paper, we present a novel unsupervised segmentation method that employs manifold learning via consensus schemes for detection of cancerous regions from high resolution 1.5 Tesla (T) endorectal in vivo prostate MRI. A significant contribution of this paper is a method to combine multiple weak, lower-dimensional representations of high dimensional feature data in a way analogous to classifier ensemble schemes, and hence create a stable and accurate reduced dimensional representation. After correcting for MR image intensity artifacts, such as bias field inhomogeneity and intensity non-standardness, our algorithm extracts over 350 3D texture features at every spatial location in the MR scene at multiple scales and orientations. Non-linear dimensionality reduction schemes such as Locally Linear Embedding (LLE) and Graph Embedding (GE) are employed to create multiple low dimensional data representations of this high dimensional texture feature space. Our novel consensus embedding method is used to average object adjacencies from within the multiple low dimensional projections so that class relationships are preserved. Unsupervised consensus clustering is then used to partition the objects in this consensus embedding space into distinct classes. Quantitative evaluation on 18 1.5 T prostate MR data against corresponding histology obtained from the multi-site ACRIN trials show a sensitivity of 92.65% and a specificity of 82.06%, which suggests that our method is successfully able to detect suspicious regions in the prostate.

Four-dimensional positron age-momentum correlation

NASA Astrophysics Data System (ADS)

Ackermann, Ulrich; Löwe, Benjamin; Dickmann, Marcel; Mitteneder, Johannes; Sperr, Peter; Egger, Werner; Reiner, Markus; Dollinger, Günther

2016-11-01

We have performed first four-dimensional age-momentum correlation (4D-AMOC) measurements at a pulsed high intensity positron micro beam and determined the absolute value of the three-dimensional momentum of the electrons annihilating with the positrons in coincidence with the positron age in the sample material. We operated two position sensitive detectors in coincidence to measure the annihilation radiation: a pixelated HPGe-detector and a microchannel plate image intensifier with a CeBr3 scintillator pixel array. The transversal momentum resolution of the 4D-AMOC setup was measured to be about 17 × 10-3 {m}0c (FWHM) and was circa 3.5 times larger than the longitudinal momentum resolution. The total time resolution was 540 ps (FWHM). We measured two samples: a gold foil and a carbon tape at a positron implantation energy of 2 keV. For each sample discrete electron momentum states and their respective positron lifetimes were extracted.

Two-Dimensional Micro-/Nanoradian Angle Generator with High Resolution and Repeatability Based on Piezo-Driven Double-Axis Flexure Hinge and Three Capacitive Sensors.

PubMed

Tan, Xinran; Zhu, Fan; Wang, Chao; Yu, Yang; Shi, Jian; Qi, Xue; Yuan, Feng; Tan, Jiubin

2017-11-19

This study presents a two-dimensional micro-/nanoradian angle generator (2D-MNAG) that achieves high angular displacement resolution and repeatability using a piezo-driven flexure hinge for two-dimensional deflections and three capacitive sensors for output angle monitoring and feedback control. The principal error of the capacitive sensor for precision microangle measurement is analyzed and compensated for; so as to achieve a high angle output resolution of 10 nrad (0.002 arcsec) and positioning repeatability of 120 nrad (0.024 arcsec) over a large angular range of ±4363 μrad (±900 arcsec) for the 2D-MNAG. The impact of each error component, together with the synthetic error of the 2D-MNAG after principal error compensation are determined using Monte Carlo simulation for further improvement of the 2D-MNAG.

Two-Dimensional Micro-/Nanoradian Angle Generator with High Resolution and Repeatability Based on Piezo-Driven Double-Axis Flexure Hinge and Three Capacitive Sensors

PubMed Central

Tan, Xinran; Zhu, Fan; Wang, Chao; Yu, Yang; Shi, Jian; Qi, Xue; Yuan, Feng; Tan, Jiubin

2017-01-01

This study presents a two-dimensional micro-/nanoradian angle generator (2D-MNAG) that achieves high angular displacement resolution and repeatability using a piezo-driven flexure hinge for two-dimensional deflections and three capacitive sensors for output angle monitoring and feedback control. The principal error of the capacitive sensor for precision microangle measurement is analyzed and compensated for; so as to achieve a high angle output resolution of 10 nrad (0.002 arcsec) and positioning repeatability of 120 nrad (0.024 arcsec) over a large angular range of ±4363 μrad (±900 arcsec) for the 2D-MNAG. The impact of each error component, together with the synthetic error of the 2D-MNAG after principal error compensation are determined using Monte Carlo simulation for further improvement of the 2D-MNAG. PMID:29156595

A two-dimensional intensified photodiode array for imaging spectroscopy

NASA Technical Reports Server (NTRS)

Tennyson, P. D.; Dymond, K.; Moos, H. W.; Feldman, P. D.; Mackey, E. F.

1986-01-01

The Johns Hopkins University is currently developing an instrument to fly aboard NASA's Space Shuttle as a Spartan payload in the late 1980s. This Spartan free flyer will obtain spatially resolved spectra of faint extended emission line objects in the wavelength range 750-1150 A at about 2-A resolution. The use of two-dimensional photon counting detectors will give simultaneous coverage of the 400 A spectral range and the 9 arc-minute spatial resolution along the spectrometer slit. The progress towards the flight detector is reported here with preliminary results from a laboratory breadboard detector, and a comparison with the one-dimensional detector developed for the Hopkins Ultraviolet Telescope. A hardware digital centroiding algorithm has been successfully implemented. The system is ultimately capable of 15-micron resolution in two dimensions at the image plane and can handle continuous counting rates of up to 8000 counts/s.

20 CFR 411.635 - Can a beneficiary be represented in the dispute resolution process under the Ticket to Work program?

Code of Federal Regulations, 2012 CFR

2012-04-01

... SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Ticket to Work Program Dispute Resolution Disputes Between Beneficiaries and Employment Networks § 411.635 Can a beneficiary be...

20 CFR 411.635 - Can a beneficiary be represented in the dispute resolution process under the Ticket to Work program?

Code of Federal Regulations, 2011 CFR

2011-04-01

... SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Ticket to Work Program Dispute Resolution Disputes Between Beneficiaries and Employment Networks § 411.635 Can a beneficiary be...

20 CFR 411.635 - Can a beneficiary be represented in the dispute resolution process under the Ticket to Work program?

Code of Federal Regulations, 2014 CFR

2014-04-01

... SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Ticket to Work Program Dispute Resolution Disputes Between Beneficiaries and Employment Networks § 411.635 Can a beneficiary be...

20 CFR 411.635 - Can a beneficiary be represented in the dispute resolution process under the Ticket to Work program?

Code of Federal Regulations, 2010 CFR

2010-04-01

... SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Ticket to Work Program Dispute Resolution Disputes Between Beneficiaries and Employment Networks § 411.635 Can a beneficiary be...

20 CFR 411.635 - Can a beneficiary be represented in the dispute resolution process under the Ticket to Work program?

Code of Federal Regulations, 2013 CFR

2013-04-01

... SOCIAL SECURITY ADMINISTRATION THE TICKET TO WORK AND SELF-SUFFICIENCY PROGRAM Ticket to Work Program Dispute Resolution Disputes Between Beneficiaries and Employment Networks § 411.635 Can a beneficiary be...

Statistical Projections for Multi-resolution, Multi-dimensional Visual Data Exploration and Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoa T. Nguyen; Stone, Daithi; E. Wes Bethel

2016-01-01

An ongoing challenge in visual exploration and analysis of large, multi-dimensional datasets is how to present useful, concise information to a user for some specific visualization tasks. Typical approaches to this problem have proposed either reduced-resolution versions of data, or projections of data, or both. These approaches still have some limitations such as consuming high computation or suffering from errors. In this work, we explore the use of a statistical metric as the basis for both projections and reduced-resolution versions of data, with a particular focus on preserving one key trait in data, namely variation. We use two different casemore » studies to explore this idea, one that uses a synthetic dataset, and another that uses a large ensemble collection produced by an atmospheric modeling code to study long-term changes in global precipitation. The primary findings of our work are that in terms of preserving the variation signal inherent in data, that using a statistical measure more faithfully preserves this key characteristic across both multi-dimensional projections and multi-resolution representations than a methodology based upon averaging.« less

3D printing from microfocus computed tomography (micro-CT) in human specimens: education and future implications.

PubMed

Shelmerdine, Susan C; Simcock, Ian C; Hutchinson, John Ciaran; Aughwane, Rosalind; Melbourne, Andrew; Nikitichev, Daniil I; Ong, Ju-Ling; Borghi, Alessandro; Cole, Garrard; Kingham, Emilia; Calder, Alistair D; Capelli, Claudio; Akhtar, Aadam; Cook, Andrew C; Schievano, Silvia; David, Anna; Ourselin, Sebastian; Sebire, Neil J; Arthurs, Owen J

2018-06-14

Microfocus CT (micro-CT) is an imaging method that provides three-dimensional digital data sets with comparable resolution to light microscopy. Although it has traditionally been used for non-destructive testing in engineering, aerospace industries and in preclinical animal studies, new applications are rapidly becoming available in the clinical setting including post-mortem fetal imaging and pathological specimen analysis. Printing three-dimensional models from imaging data sets for educational purposes is well established in the medical literature, but typically using low resolution (0.7 mm voxel size) data acquired from CT or MR examinations. With higher resolution imaging (voxel sizes below 1 micron, <0.001 mm) at micro-CT, smaller structures can be better characterised, and data sets post-processed to create accurate anatomical models for review and handling. In this review, we provide examples of how three-dimensional printing of micro-CT imaged specimens can provide insight into craniofacial surgical applications, developmental cardiac anatomy, placental imaging, archaeological remains and high-resolution bone imaging. We conclude with other potential future usages of this emerging technique.

Attenuation tomography of the main volcanic regions of the Campanian Plain.

NASA Astrophysics Data System (ADS)

de Siena, Luca; Del Pezzo, Edoardo; Bianco, Francesca

2010-05-01

Passive, high resolution attenuation tomography is used to image the geological structure in the first upper 4 km of shallow crust beneath the Campanian Plain. Images were produced by two separate attenuation tomography studies of the main volcanic regions of the Campanian Plain, Southern Italy, Mt. Vesuvius volcano and Campi Flegrei caldera. The three-dimensional S wave attenuation tomography of Mt. Vesuvius has been obtained with multiple measurements of coda-normalized S-wave spectra of local small magnitude earthquakes. P-wave attenuation tomography was performed using classical spectral methods. The images were obtained inverting the spectral data with a multiple resolution approach expressively designed for attenuation tomography. This allowed to obtain a robust attenuation image of the volumes under the central cone at a maximum resolution of 300 m. The same approach was applied to a data set recorded in the Campi Flegrei area during the 1982-1984 seismic crisis. Inversion ensures a minimum cell size resolution of 500 meters in the zones with sufficient ray coverage, and 1000 meters outside these zones. The study of the resolution matrix as well as the synthetic tests guarantee an optimal reproduction of the input anomalies in the center of the caldera, between 0 and 3.5 km in depth. Results allowed an unprecedented view of several features of the medium, like the residual part of solidified magma from the last eruption, under the central cone of Mt. Vesuvius, and the feeding systems and top of the carbonate basement, 3 km depth below both volcanic areas. Vertical Q contrast image important fault zones, such as the La Starza fault, as well as high attenuation structures that correspond to gas or fluid reservoirs, and reveal the upper part of gas bearing conduits connecting these high attenuation volumes with the magma sill revealed at about 7 km in depth by passive travel-time tomography under the whole Campanian Plain.

Automated Detection of Salt Marsh Platforms : a Topographic Method

NASA Astrophysics Data System (ADS)

Goodwin, G.; Mudd, S. M.; Clubb, F. J.

2017-12-01

Monitoring the topographic evolution of coastal marshes is a crucial step toward improving the management of these valuable landscapes under the pressure of relative sea level rise and anthropogenic modification. However, determining their geometrically complex boundaries currently relies on spectral vegetation detection methods or requires labour-intensive field surveys and digitisation.We propose a novel method to reproducibly isolate saltmarsh scarps and platforms from a DEM. Field observations and numerical models show that saltmarshes mature into sub-horizontal platforms delineated by sub-vertical scarps: based on this premise, we identify scarps as lines of local maxima on a slope*relief raster, then fill landmasses from the scarps upward, thus isolating mature marsh platforms. Non-dimensional search parameters allow batch-processing of data without recalibration. We test our method using lidar-derived DEMs of six saltmarshes in England with varying tidal ranges and geometries, for which topographic platforms were manually isolated from tidal flats. Agreement between manual and automatic segregation exceeds 90% for resolutions of 1m, with all but one sites maintaining this performance for resolutions up to 3.5m. For resolutions of 1m, automatically detected platforms are comparable in surface area and elevation distribution to digitised platforms. We also find that our method allows the accurate detection of local bloc failures 3 times larger than the DEM resolution.Detailed inspection reveals that although tidal creeks were digitised as part of the marsh platform, automatic detection classifies them as part of the tidal flat, causing an increase in false negatives and overall platform perimeter. This suggests our method would benefit from a combination with existing creek detection algorithms. Fallen blocs and pioneer zones are inconsistently identified, particularly in macro-tidal marshes, leading to differences between digitisation and the automated method: this also suggests that these areas must be carefully considered when analysing erosion and accretion processes. Ultimately, we have shown that automatic detection of marsh platforms from high-resolution topography is possible and sufficient to monitor and analyse topographic evolution.

Sub-cycle light transients for attosecond, X-ray, four-dimensional imaging

NASA Astrophysics Data System (ADS)

Fattahi, Hanieh

2016-10-01

This paper reviews the revolutionary development of ultra-short, multi-TW laser pulse generation made possible by current laser technology. The design of the unified laser architecture discussed in this paper, based on the synthesis of ultrabroadband optical parametric chirped-pulse amplifiers, promises to provide powerful light transients with electromagnetic forces engineerable on the electron time scale. By coherent combination of multiple amplifiers operating in different wavelength ranges, pulses with wavelength spectra extending from less than 1 ?m to more than 10 ?m, with sub-cycle duration at unprecedented peak and average power levels can be generated. It is shown theoretically that these light transients enable the efficient generation of attosecond X-ray pulses with photon flux sufficient to image, for the first time, picometre-attosecond trajectories of electrons, by means of X-ray diffraction and record the electron dynamics by attosecond spectroscopy. The proposed system leads to a tool with sub-atomic spatio-temporal resolution for studying different processes deep inside matter.

INFFTM: Fast evaluation of 3d Fourier series in MATLAB with an application to quantum vortex reconnections

NASA Astrophysics Data System (ADS)

Caliari, Marco; Zuccher, Simone

2017-04-01

Although Fourier series approximation is ubiquitous in computational physics owing to the Fast Fourier Transform (FFT) algorithm, efficient techniques for the fast evaluation of a three-dimensional truncated Fourier series at a set of arbitrary points are quite rare, especially in MATLAB language. Here we employ the Nonequispaced Fast Fourier Transform (NFFT, by J. Keiner, S. Kunis, and D. Potts), a C library designed for this purpose, and provide a Matlab® and GNU Octave interface that makes NFFT easily available to the Numerical Analysis community. We test the effectiveness of our package in the framework of quantum vortex reconnections, where pseudospectral Fourier methods are commonly used and local high resolution is required in the post-processing stage. We show that the efficient evaluation of a truncated Fourier series at arbitrary points provides excellent results at a computational cost much smaller than carrying out a numerical simulation of the problem on a sufficiently fine regular grid that can reproduce comparable details of the reconnecting vortices.

Model selection and Bayesian inference for high-resolution seabed reflection inversion.

PubMed

Dettmer, Jan; Dosso, Stan E; Holland, Charles W

2009-02-01

This paper applies Bayesian inference, including model selection and posterior parameter inference, to inversion of seabed reflection data to resolve sediment structure at a spatial scale below the pulse length of the acoustic source. A practical approach to model selection is used, employing the Bayesian information criterion to decide on the number of sediment layers needed to sufficiently fit the data while satisfying parsimony to avoid overparametrization. Posterior parameter inference is carried out using an efficient Metropolis-Hastings algorithm for high-dimensional models, and results are presented as marginal-probability depth distributions for sound velocity, density, and attenuation. The approach is applied to plane-wave reflection-coefficient inversion of single-bounce data collected on the Malta Plateau, Mediterranean Sea, which indicate complex fine structure close to the water-sediment interface. This fine structure is resolved in the geoacoustic inversion results in terms of four layers within the upper meter of sediments. The inversion results are in good agreement with parameter estimates from a gravity core taken at the experiment site.

Polar Wind Measurements with TIDE/PSI and HYDRA on the Polar Spacecraft

NASA Technical Reports Server (NTRS)

Su, Y. J.; Horwitz, J. L.; Moore, Thomas E.; Giles, Barbara L.; Chandler, Michael O.; Craven, Paul D.; Chang, S.-W.; Scudder, J.

1998-01-01

The Thermal Ion Dynamics Experiment (TIDE) on the POLAR spacecraft has allowed sampling of the three-dimensional ion distributions with excellent energy, angular, and mass resolution. The companion Plasma Source Instrument, when operated, allows sufficient diminution of the electric potential to observe the polar wind at very high altitudes. In this presentation, we will describe the results of polar wind characteristics H+, He+, and 0+ as observed by TIDE at 5000 km and 8 RE altitudes. The relationship of the polar wind parameters with the solar zenith angle and with the day-night distance in the Solar Magnetic coordinate system will also be presented. We will compare these measurements with recent simulations of the photoelectron-driven polar wind using a couple fluid-semikinetic model. In addition, we will compare these polar wind observations with low-energy electrons sampled by the HYDRA experiment on POLAR to examine possible effects of the polar rain and photoelectrons and hopefully explain the large ion outflow velocity variations at POLAR apogee.

Sequence co-evolution gives 3D contacts and structures of protein complexes

PubMed Central

Hopf, Thomas A; Schärfe, Charlotta P I; Rodrigues, João P G L M; Green, Anna G; Kohlbacher, Oliver; Sander, Chris; Bonvin, Alexandre M J J; Marks, Debora S

2014-01-01

Protein–protein interactions are fundamental to many biological processes. Experimental screens have identified tens of thousands of interactions, and structural biology has provided detailed functional insight for select 3D protein complexes. An alternative rich source of information about protein interactions is the evolutionary sequence record. Building on earlier work, we show that analysis of correlated evolutionary sequence changes across proteins identifies residues that are close in space with sufficient accuracy to determine the three-dimensional structure of the protein complexes. We evaluate prediction performance in blinded tests on 76 complexes of known 3D structure, predict protein–protein contacts in 32 complexes of unknown structure, and demonstrate how evolutionary couplings can be used to distinguish between interacting and non-interacting protein pairs in a large complex. With the current growth of sequences, we expect that the method can be generalized to genome-wide elucidation of protein–protein interaction networks and used for interaction predictions at residue resolution. DOI: http://dx.doi.org/10.7554/eLife.03430.001 PMID:25255213

Description of a prototype emission-transmission computed tomography imaging system

NASA Technical Reports Server (NTRS)

Lang, T. F.; Hasegawa, B. H.; Liew, S. C.; Brown, J. K.; Blankespoor, S. C.; Reilly, S. M.; Gingold, E. L.; Cann, C. E.

1992-01-01

We have developed a prototype imaging system that can perform simultaneous x-ray transmission CT and SPECT phantom studies. This system employs a 23-element high-purity-germanium detector array. The detector array is coupled to a collimator with septa angled toward the focal spot of an x-ray tube. During image acquisition, the x-ray fan beam and the detector array move synchronously along an arc pivoted at the x-ray source. Multiple projections are obtained by rotating the object, which is mounted at the center of rotation of the system. The detector array and electronics can count up to 10(6) cps/element with sufficient energy-resolution to discriminate between x-rays at 100-120 kVp and gamma rays from 99mTc. We have used this device to acquire x-ray CT and SPECT images of a three-dimensional Hoffman brain phantom. The emission and transmission images may be superimposed in order to localize the emission image on the transmission map.

3D visualization of subcellular structures of Schizosaccharomyces pombe by hard X-ray tomography.

PubMed

Yang, Y; Li, W; Liu, G; Zhang, X; Chen, J; Wu, W; Guan, Y; Xiong, Y; Tian, Y; Wu, Z

2010-10-01

Cellular structures of the fission yeast, Schizosaccharomyces pombe, were examined by using hard X-ray tomography. Since cells are nearly transparent to hard X-rays, Zernike phase contrast and heavy metal staining were introduced to improve image contrast. Through using such methods, images taken at 8 keV displayed sufficient contrast for observing cellular structures. The cell wall, the intracellular organelles and the entire structural organization of the whole cells were visualized in three-dimensional at a resolution better than 100 nm. Comparison between phase contrast and absorption contrast was also made, indicating the obvious advantage of phase contrast for cellular imaging at this energy. Our results demonstrate that hard X-ray tomography with Zernike phase contrast is suitable for cellular imaging. Its unique abilities make it have potential to become a useful tool for revealing structural information from cells, especially thick eukaryotic cells. © 2010 The Authors Journal compilation © 2010 The Royal Microscopical Society.

Nondestructive measurement of an optical fiber refractive-index profile by a transmitted-light differential interference contact microscope.

PubMed

Liu, Zhongyao; Dong, Xiaoman; Chen, Qianghua; Yin, Chunyong; Xu, Yuxian; Zheng, Yingjun

2004-03-01

A novel transmitted-light differential interference contrast (DIC) system is used for nondestructive measurement of the refractive-index profile (RIP) of an optical fiber. By means of this system the phase of a measured light beam can be modulated with an analyzer, and the phase distribution of a fiber is obtained by calculation of the various interference patterns. The measurement theory and structure and some typical applications of this system are demonstrated. The results of measuring RIPs in graded-index fiber are presented. Both the experimental results and theoretical analysis show that the system takes the advantage of high index resolution and of sufficient measurement accuracy for measuring the refractive index of the optical fiber. The system has strong ability to overcome environmental disturbance because of its common-path design. Moreover, one can use the system to measure the RIP along the fiber axis and acquire an image of the three-dimensional RIP of the fiber.

Compact three-dimensional super-resolution system based on fluorescence emission difference microscopy

NASA Astrophysics Data System (ADS)

Zhu, Dazhao; Chen, Youhua; Fang, Yue; Hussain, Anwar; Kuang, Cuifang; Zhou, Xiaoxu; Xu, Yingke; Liu, Xu

2017-12-01

A compact microscope system for three-dimensional (3-D) super-resolution imaging is presented. The super-resolution capability of the system is based on a size-reduced effective 3-D point spread function generated through the fluorescence emission difference (FED) method. The appropriate polarization direction distribution and manipulation allows the panel active area of the spatial light modulator to be fully utilized. This allows simultaneous modulation of the incident light by two kinds of phase masks to be performed with a single spatial light modulator in order to generate a 3-D negative spot. The system is more compact than standard 3-D FED systems while maintaining all the advantages of 3-D FED microscopy. The experimental results demonstrated the improvement in 3-D resolution by nearly 1.7 times and 1.6 times compared to the classic confocal resolution in the lateral and axial directions, respectively.

Three-dimensional super-resolved live cell imaging through polarized multi-angle TIRF.

PubMed

Zheng, Cheng; Zhao, Guangyuan; Liu, Wenjie; Chen, Youhua; Zhang, Zhimin; Jin, Luhong; Xu, Yingke; Kuang, Cuifang; Liu, Xu

2018-04-01

Measuring three-dimensional nanoscale cellular structures is challenging, especially when the structure is dynamic. Owing to the informative total internal reflection fluorescence (TIRF) imaging under varied illumination angles, multi-angle (MA) TIRF has been examined to offer a nanoscale axial and a subsecond temporal resolution. However, conventional MA-TIRF still performs badly in lateral resolution and fails to characterize the depth image in densely distributed regions. Here, we emphasize the lateral super-resolution in the MA-TIRF, exampled by simply introducing polarization modulation into the illumination procedure. Equipped with a sparsity and accelerated proximal algorithm, we examine a more precise 3D sample structure compared with previous methods, enabling live cell imaging with a temporal resolution of 2 s and recovering high-resolution mitochondria fission and fusion processes. We also shared the recovery program, which is the first open-source recovery code for MA-TIRF, to the best of our knowledge.

A resolution measure for three-dimensional microscopy

PubMed Central

Chao, Jerry; Ram, Sripad; Abraham, Anish V.; Ward, E. Sally; Ober, Raimund J.

2009-01-01

A three-dimensional (3D) resolution measure for the conventional optical microscope is introduced which overcomes the drawbacks of the classical 3D (axial) resolution limit. Formulated within the context of a parameter estimation problem and based on the Cramer-Rao lower bound, this 3D resolution measure indicates the accuracy with which a given distance between two objects in 3D space can be determined from the acquired image. It predicts that, given enough photons from the objects of interest, arbitrarily small distances of separation can be estimated with prespecified accuracy. Using simulated images of point source pairs, we show that the maximum likelihood estimator is capable of attaining the accuracy predicted by the resolution measure. We also demonstrate how different factors, such as extraneous noise sources and the spatial orientation of the imaged object pair, can affect the accuracy with which a given distance of separation can be determined. PMID:20161040

High-resolution structure of viruses from random diffraction snapshots

PubMed Central

Hosseinizadeh, A.; Schwander, P.; Dashti, A.; Fung, R.; D'Souza, R. M.; Ourmazd, A.

2014-01-01

The advent of the X-ray free-electron laser (XFEL) has made it possible to record diffraction snapshots of biological entities injected into the X-ray beam before the onset of radiation damage. Algorithmic means must then be used to determine the snapshot orientations and thence the three-dimensional structure of the object. Existing Bayesian approaches are limited in reconstruction resolution typically to 1/10 of the object diameter, with the computational expense increasing as the eighth power of the ratio of diameter to resolution. We present an approach capable of exploiting object symmetries to recover three-dimensional structure to high resolution, and thus reconstruct the structure of the satellite tobacco necrosis virus to atomic level. Our approach offers the highest reconstruction resolution for XFEL snapshots to date and provides a potentially powerful alternative route for analysis of data from crystalline and nano-crystalline objects. PMID:24914154

High-resolution structure of viruses from random diffraction snapshots.

PubMed

Hosseinizadeh, A; Schwander, P; Dashti, A; Fung, R; D'Souza, R M; Ourmazd, A

2014-07-17

The advent of the X-ray free-electron laser (XFEL) has made it possible to record diffraction snapshots of biological entities injected into the X-ray beam before the onset of radiation damage. Algorithmic means must then be used to determine the snapshot orientations and thence the three-dimensional structure of the object. Existing Bayesian approaches are limited in reconstruction resolution typically to 1/10 of the object diameter, with the computational expense increasing as the eighth power of the ratio of diameter to resolution. We present an approach capable of exploiting object symmetries to recover three-dimensional structure to high resolution, and thus reconstruct the structure of the satellite tobacco necrosis virus to atomic level. Our approach offers the highest reconstruction resolution for XFEL snapshots to date and provides a potentially powerful alternative route for analysis of data from crystalline and nano-crystalline objects.

System design of a small OpenPET prototype with 4-layer DOI detectors.

PubMed

Yoshida, Eiji; Kinouchi, Shoko; Tashima, Hideaki; Nishikido, Fumihiko; Inadama, Naoko; Murayama, Hideo; Yamaya, Taiga

2012-01-01

We have proposed an OpenPET geometry which consists of two axially separated detector rings. The open gap is suitable for in-beam PET. We have developed the small prototype of the OpenPET especially for a proof of concept of in-beam imaging. This paper presents an overview of the main features implemented in this prototype. We also evaluated the detector performance. This prototype was designed with 2 detector rings having 8 depth-of-interaction detectors. Each detector consisted of 784 Lu(2x)Gd(2(1-x))SiO₅:Ce (LGSO) which were arranged in a 4-layer design, coupled to a position-sensitive photomultiplier tube (PS-PMT). The size of the LGSO array was smaller than the sensitive area of the PS-PMT, so that we could obtain sufficient LGSO identification. Peripheral LGSOs near the open gap directly detect the gamma rays on the side face in the OpenPET geometry. Output signals of two detectors stacked axially were projected onto one 2-dimensional position histogram for reduction of the scale of a coincidence processor. Front-end circuits were separated from the detector head by 1.2-m coaxial cables for the protection of electronic circuits from radiation damage. The detectors had sufficient crystal identification capability. Cross talk between the combined two detectors could be ignored. The timing and energy resolutions were 3.0 ns and 14%, respectively. The coincidence window was set 20 ns, because the timing histogram showed that not only the main peak, but also two small shifted peaks were caused by the coaxial cable. However, the detector offers the promise of sufficient performance, because random coincidences are at a nearly undetectable level for in-beam PET experiments.

Development of high-resolution x-ray CT system using parallel beam geometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yoneyama, Akio, E-mail: akio.yoneyama.bu@hitachi.com; Baba, Rika; Hyodo, Kazuyuki

2016-01-28

For fine three-dimensional observations of large biomedical and organic material samples, we developed a high-resolution X-ray CT system. The system consists of a sample positioner, a 5-μm scintillator, microscopy lenses, and a water-cooled sCMOS detector. Parallel beam geometry was adopted to attain a field of view of a few mm square. A fine three-dimensional image of birch branch was obtained using a 9-keV X-ray at BL16XU of SPring-8 in Japan. The spatial resolution estimated from the line profile of a sectional image was about 3 μm.

In-line three-dimensional holography of nanocrystalline objects at atomic resolution

DOE PAGES

Chen, F. -R.; Van Dyck, D.; Kisielowski, C.

2016-02-18

We report that resolution and sensitivity of the latest generation aberration-corrected transmission electron microscopes allow the vast majority of single atoms to be imaged with sub-Ångstrom resolution and their locations determined in an image plane with a precision that exceeds the 1.9-pm wavelength of 300 kV electrons. Such unprecedented performance allows expansion of electron microscopic investigations with atomic resolution into the third dimension. Here we show a general tomographic method to recover the three-dimensional shape of a crystalline particle from high-resolution images of a single projection without the need for sample rotation. The method is compatible with low dose ratemore » electron microscopy, which improves on signal quality, while minimizing electron beam-induced structure modifications even for small particles or surfaces. Lastly, we apply it to germanium, gold and magnesium oxide particles, and achieve a depth resolution of 1–2 Å, which is smaller than inter-atomic distances.« less

Computer programs for calculating two-dimensional potential flow in and about propulsion system inlets

NASA Technical Reports Server (NTRS)

Hawk, J. D.; Stockman, N. O.; Farrell, C. A., Jr.

1978-01-01

Incompressible potential flow calculations are presented that were corrected for compressibility in two-dimensional inlets at arbitrary operating conditions. Included are a statement of the problem to be solved, a description of each of the computer programs, and sufficient documentation, including a test case, to enable a user to run the program.

Computer programs for calculating two-dimensional potential flow through deflected nozzles

NASA Technical Reports Server (NTRS)

Hawk, J. D.; Stockman, N. O.

1979-01-01

Computer programs to calculate the incompressible potential flow, corrected for compressibility, in two-dimensional nozzles at arbitrary operating conditions are presented. A statement of the problem to be solved, a description of each of the computer programs, and sufficient documentation, including a test case, to enable a user to run the program are included.

Single-particle cryo-EM using alignment by classification (ABC): the structure of Lumbricus terrestris haemoglobin.

PubMed

Afanasyev, Pavel; Seer-Linnemayr, Charlotte; Ravelli, Raimond B G; Matadeen, Rishi; De Carlo, Sacha; Alewijnse, Bart; Portugal, Rodrigo V; Pannu, Navraj S; Schatz, Michael; van Heel, Marin

2017-09-01

Single-particle cryogenic electron microscopy (cryo-EM) can now yield near-atomic resolution structures of biological complexes. However, the reference-based alignment algorithms commonly used in cryo-EM suffer from reference bias, limiting their applicability (also known as the 'Einstein from random noise' problem). Low-dose cryo-EM therefore requires robust and objective approaches to reveal the structural information contained in the extremely noisy data, especially when dealing with small structures. A reference-free pipeline is presented for obtaining near-atomic resolution three-dimensional reconstructions from heterogeneous ('four-dimensional') cryo-EM data sets. The methodologies integrated in this pipeline include a posteriori camera correction, movie-based full-data-set contrast transfer function determination, movie-alignment algorithms, (Fourier-space) multivariate statistical data compression and unsupervised classification, 'random-startup' three-dimensional reconstructions, four-dimensional structural refinements and Fourier shell correlation criteria for evaluating anisotropic resolution. The procedures exclusively use information emerging from the data set itself, without external 'starting models'. Euler-angle assignments are performed by angular reconstitution rather than by the inherently slower projection-matching approaches. The comprehensive 'ABC-4D' pipeline is based on the two-dimensional reference-free 'alignment by classification' (ABC) approach, where similar images in similar orientations are grouped by unsupervised classification. Some fundamental differences between X-ray crystallography versus single-particle cryo-EM data collection and data processing are discussed. The structure of the giant haemoglobin from Lumbricus terrestris at a global resolution of ∼3.8 Å is presented as an example of the use of the ABC-4D procedure.

Confocal Imaging of porous media

NASA Astrophysics Data System (ADS)

Shah, S.; Crawshaw, D.; Boek, D.

2012-12-01

Carbonate rocks, which hold approximately 50% of the world's oil and gas reserves, have a very complicated and heterogeneous structure in comparison with sandstone reservoir rock. We present advances with different techniques to image, reconstruct, and characterize statistically the micro-geometry of carbonate pores. The main goal here is to develop a technique to obtain two dimensional and three dimensional images using Confocal Laser Scanning Microscopy. CLSM is used in epi-fluorescent imaging mode, allowing for the very high optical resolution of features well below 1μm size. Images of pore structures were captured using CLSM imaging where spaces in the carbonate samples were impregnated with a fluorescent, dyed epoxy-resin, and scanned in the x-y plane by a laser probe. We discuss the sample preparation in detail for Confocal Imaging to obtain sub-micron resolution images of heterogeneous carbonate rocks. We also discuss the technical and practical aspects of this imaging technique, including its advantages and limitation. We present several examples of this application, including studying pore geometry in carbonates, characterizing sub-resolution porosity in two dimensional images. We then describe approaches to extract statistical information about porosity using image processing and spatial correlation function. We have managed to obtain very low depth information in z -axis (~ 50μm) to develop three dimensional images of carbonate rocks with the current capabilities and limitation of CLSM technique. Hence, we have planned a novel technique to obtain higher depth information to obtain high three dimensional images with sub-micron resolution possible in the lateral and axial planes.

Three-dimensional reconstruction of frozen and thawed plant tissues from microscopic images

USDA-ARS?s Scientific Manuscript database

Histological analysis of frozen and thawed plants has been conducted for many years but the observation of individual sections only provides a 2 dimensional representation of a 3 dimensional phenomenon. Most techniques for viewing internal plant structure in 3 dimensions is either low in resolution...

Global Hopf bifurcation analysis on a BAM neural network with delays

NASA Astrophysics Data System (ADS)

Sun, Chengjun; Han, Maoan; Pang, Xiaoming

2007-01-01

A delayed differential equation that models a bidirectional associative memory (BAM) neural network with four neurons is considered. By using a global Hopf bifurcation theorem for FDE and a Bendixon's criterion for high-dimensional ODE, a group of sufficient conditions for the system to have multiple periodic solutions are obtained when the sum of delays is sufficiently large.

Synthetic 3D diamond-based electrodes for flexible retinal neuroprostheses: Model, production and in vivo biocompatibility.

PubMed

Bendali, Amel; Rousseau, Lionel; Lissorgues, Gaëlle; Scorsone, Emmanuel; Djilas, Milan; Dégardin, Julie; Dubus, Elisabeth; Fouquet, Stéphane; Benosman, Ryad; Bergonzo, Philippe; Sahel, José-Alain; Picaud, Serge

2015-10-01

Two retinal implants have recently received the CE mark and one has obtained FDA approval for the restoration of useful vision in blind patients. Since the spatial resolution of current vision prostheses is not sufficient for most patients to detect faces or perform activities of daily living, more electrodes with less crosstalk are needed to transfer complex images to the retina. In this study, we modelled planar and three-dimensional (3D) implants with a distant ground or a ground grid, to demonstrate greater spatial resolution with 3D structures. Using such flexible 3D implant prototypes, we showed that the degenerated retina could mould itself to the inside of the wells, thereby isolating bipolar neurons for specific, independent stimulation. To investigate the in vivo biocompatibility of diamond as an electrode or an isolating material, we developed a procedure for depositing diamond onto flexible 3D retinal implants. Taking polyimide 3D implants as a reference, we compared the number of neurones integrating the 3D diamond structures and their ratio to the numbers of all cells, including glial cells. Bipolar neurones were increased whereas there was no increase even a decrease in the total cell number. SEM examinations of implants confirmed the stability of the diamond after its implantation in vivo. This study further demonstrates the potential of 3D designs for increasing the resolution of retinal implants and validates the safety of diamond materials for retinal implants and neuroprostheses in general. Copyright © 2015. Published by Elsevier Ltd.

Temporal and spatial resolution required for imaging myocardial function

NASA Astrophysics Data System (ADS)

Eusemann, Christian D.; Robb, Richard A.

2004-05-01

4-D functional analysis of myocardial mechanics is an area of significant interest and research in cardiology and vascular/interventional radiology. Current multidimensional analysis is limited by insufficient temporal resolution of x-ray and magnetic resonance based techniques, but recent improvements in system design holds hope for faster and higher resolution scans to improve images of moving structures allowing more accurate functional studies, such as in the heart. This paper provides a basis for the requisite temporal and spatial resolution for useful imaging during individual segments of the cardiac cycle. Multiple sample rates during systole and diastole are compared to determine an adequate sample frequency to reduce regional myocardial tracking errors. Concurrently, out-of-plane resolution has to be sufficiently high to minimize partial volume effect. Temporal resolution and out-of-plane spatial resolution are related factors that must be considered together. The data used for this study is a DSR dynamic volume image dataset with high temporal and spatial resolution using implanted fiducial markers to track myocardial motion. The results of this study suggest a reduced exposure and scan time for x-ray and magnetic resonance imaging methods, since a lower sample rate during systole is sufficient, whereas the period of rapid filling during diastole requires higher sampling. This could potentially reduce the cost of these procedures and allow higher patient throughput.

Optical sample-position sensing for electrostatic levitation

NASA Technical Reports Server (NTRS)

Sridharan, G.; Chung, S.; Elleman, D.; Whim, W. K.

1989-01-01

A comparative study is conducted for optical position-sensing techniques applicable to micro-G conditions sample-levitation systems. CCD sensors are compared with one- and two-dimensional position detectors used in electrostatic particle levitation. In principle, the CCD camera method can be improved from current resolution levels of 200 microns through the incorporation of a higher-pixel device and more complex digital signal processor interface. Nevertheless, the one-dimensional position detectors exhibited superior, better-than-one-micron resolution.

Coherent three-dimensional X-ray cryo-imaging.

PubMed

Robinson, Ian

2015-09-01

The combination of cryogenic sample temperatures with three-dimensional coherent diffractive imaging for the case of whole frozen-hydrated cells is discussed in the light of theoretical predictions of the achievable resolution.

Three-Dimensional Orientation of Anisotropic Plasmonic Aggregates at Intracellular Nuclear Indentation Sites by Integrated Light Sheet Super-Resolution Microscopy.

PubMed

Chakkarapani, Suresh Kumar; Sun, Yucheng; Lee, Seungah; Fang, Ning; Kang, Seong Ho

2018-05-22

Three-dimensional (3D) orientations of individual anisotropic plasmonic nanoparticles in aggregates were observed in real time by integrated light sheet super-resolution microscopy ( iLSRM). Asymmetric light scattering of a gold nanorod (AuNR) was used to trigger signals based on the polarizer angle. Controlled photoswitching was achieved by turning the polarizer and obtaining a series of images at different polarization directions. 3D subdiffraction-limited super-resolution images were obtained by superlocalization of scattering signals as a function of the anisotropic optical properties of AuNRs. Varying the polarizer angle allowed resolution of the orientation of individual AuNRs. 3D images of individual nanoparticles were resolved in aggregated regions, resulting in as low as 64 nm axial resolution and 28 nm spatial resolution. The proposed imaging setup and localization approach demonstrates a convenient method for imaging under a noisy environment where the majority of scattering noise comes from cellular components. This integrated 3D iLSRM and localization technique was shown to be reliable and useful in the field of 3D nonfluorescence super-resolution imaging.

Unconventional signal detection techniques with Gaussian probability mixtures adaptation in non-AWGN channels: full resolution receiver

NASA Astrophysics Data System (ADS)

Chabdarov, Shamil M.; Nadeev, Adel F.; Chickrin, Dmitry E.; Faizullin, Rashid R.

2011-04-01

In this paper we discuss unconventional detection technique also known as «full resolution receiver». This receiver uses Gaussian probability mixtures for interference structure adaptation. Full resolution receiver is alternative to conventional matched filter receivers in the case of non-Gaussian interferences. For the DS-CDMA forward channel with presence of complex interferences sufficient performance increasing was shown.

A technique for enhancing and matching the resolution of microwave measurements from the SSM/I instrument

NASA Technical Reports Server (NTRS)

Robinson, Wayne D.; Kummerrow, Christian; Olson, William S.

1992-01-01

A correction technique is presented for matching the resolution of all the frequencies of the satelliteborne Special Sensor Microwave/Imager (SSM/I) to the about-25-km spatial resolution of the 37-GHz channel. This entails, on the one hand, the enhancement of the spatial resolution of the 19- and 22-GHz channels, and on the other, the degrading of that of the 85-GHz channel. The Backus and Gilbert (1970) approach is found to yield sufficient spatial resolution to render such a correction worthwhile.

Prospects for Electron Imaging with Ultrafast Time Resolution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Armstrong, M R; Reed, B W; Torralva, B R

2007-01-26

Many pivotal aspects of material science, biomechanics, and chemistry would benefit from nanometer imaging with ultrafast time resolution. Here we demonstrate the feasibility of short-pulse electron imaging with t10 nanometer/10 picosecond spatio-temporal resolution, sufficient to characterize phenomena that propagate at the speed of sound in materials (1-10 kilometer/second) without smearing. We outline resolution-degrading effects that occur at high current density followed by strategies to mitigate these effects. Finally, we present a model electron imaging system that achieves 10 nanometer/10 picosecond spatio-temporal resolution.

Picometer resolution interferometric characterization of the dimensional stability of zero CTE CFRP

NASA Astrophysics Data System (ADS)

Cordero Machado, Jorge; Heinrich, Thomas; Schuldt, Thilo; Gohlke, Martin; Lucarelli, Stefano; Weise, Dennis; Johann, Ulrich; Peters, Achim; Braxmaier, Claus

2008-07-01

Highly stable but lightweight structural materials are essential for the realization of spaceborne optical instruments, for example telescopes. In terms of optical performance, usually tight tolerances on the absolute spacing between telescope mirrors have to be maintained from integration on ground to operation in final orbit. Furthermore, a certain stability of the telescope structure must typically be ensured in the measurement band. Particular challenging requirements have to be met for the LISA Mission (Laser Interferometer Space Antenna), where the spacing between primary and secondary mirror must be stable to a few picometers. Only few materials offer sufficient thermal stability to provide such performance. Candidates are for example Zerodur and Carbon-Fiber Reinforced Plastic (CFRP), where the latter is preferred in terms of mechanical stiffness and robustness. We are currently investigating the suitability of CFRP with respect to the LISA requirements by characterization of its dimensional stability with heterodyne laser interferometry. The special, highly symmetric interferometer setup offers a noise level of 2 pm/√Hz at 0.1Hz and above, and therefore represents a unique tool for this purpose. Various procedures for the determination of the coefficient of thermal expansion (CTE) have been investigated, both on a test sample with negative CTE, as well as on a CFRP tube specifically tuned to provide a theoretical zero expansion in the axial dimension.

Three-dimensional locations of gold-labeled proteins in a whole mount eukaryotic cell obtained with 3nm precision using aberration-corrected scanning transmission electron microscopy.

PubMed

Dukes, Madeline J; Ramachandra, Ranjan; Baudoin, Jean-Pierre; Gray Jerome, W; de Jonge, Niels

2011-06-01

Three-dimensional (3D) maps of proteins within the context of whole cells are important for investigating cellular function. However, 3D reconstructions of whole cells are challenging to obtain using conventional transmission electron microscopy (TEM). We describe a methodology to determine the 3D locations of proteins labeled with gold nanoparticles on whole eukaryotic cells. The epidermal growth factor receptors on COS7 cells were labeled with gold nanoparticles, and critical-point dried whole-mount cell samples were prepared. 3D focal series were obtained with aberration-corrected scanning transmission electron microscopy (STEM), without tilting the specimen. The axial resolution was improved with deconvolution. The vertical locations of the nanoparticles in a whole-mount cell were determined with a precision of 3nm. From the analysis of the variation of the axial positions of the labels we concluded that the cellular surface was ruffled. To achieve sufficient stability of the sample under electron beam irradiation during the recording of the focal series, the sample was carbon coated. A quantitative method was developed to analyze the stability of the ultrastructure after electron beam irradiation using TEM. The results of this study demonstrate the feasibility of using aberration-corrected STEM to study the 3D nanoparticle distribution in whole cells. Copyright © 2011 Elsevier Inc. All rights reserved.

Extrinsic Calibration of a Laser Galvanometric Setup and a Range Camera.

PubMed

Sels, Seppe; Bogaerts, Boris; Vanlanduit, Steve; Penne, Rudi

2018-05-08

Currently, galvanometric scanning systems (like the one used in a scanning laser Doppler vibrometer) rely on a planar calibration procedure between a two-dimensional (2D) camera and the laser galvanometric scanning system to automatically aim a laser beam at a particular point on an object. In the case of nonplanar or moving objects, this calibration is not sufficiently accurate anymore. In this work, a three-dimensional (3D) calibration procedure that uses a 3D range sensor is proposed. The 3D calibration is valid for all types of objects and retains its accuracy when objects are moved between subsequent measurement campaigns. The proposed 3D calibration uses a Non-Perspective-n-Point (NPnP) problem solution. The 3D range sensor is used to calculate the position of the object under test relative to the laser galvanometric system. With this extrinsic calibration, the laser galvanometric scanning system can automatically aim a laser beam to this object. In experiments, the mean accuracy of aiming the laser beam on an object is below 10 mm for 95% of the measurements. This achieved accuracy is mainly determined by the accuracy and resolution of the 3D range sensor. The new calibration method is significantly better than the original 2D calibration method, which in our setup achieves errors below 68 mm for 95% of the measurements.

Three-dimensional locations of gold-labeled proteins in a whole mount eukaryotic cell obtained with 3 nm precision using aberration-corrected scanning transmission electron microscopy

PubMed Central

Dukes, Madeline J.; Ramachandra, Ranjan; Baudoin, Jean-Pierre; Jerome, W. Gray; de Jonge, Niels

2011-01-01

Three-dimensional (3D) maps of proteins within the context of whole cells are important for investigating cellular function. However, 3D reconstructions of whole cells are challenging to obtain using conventional transmission electron microscopy (TEM). We describe a methodology to determine the 3D locations of proteins labeled with gold nanoparticles on whole eukaryotic cells. The epidermal growth factor receptors on COS7 cells were labeled with gold nanoparticles, and critical-point dried whole-mount cell samples were prepared. 3D focal series were obtained with aberration-corrected scanning transmission electron microscopy (STEM), without tilting the specimen. The axial resolution was improved with deconvolution. The vertical locations of the nanoparticles in a whole-mount cell were determined with a precision of 3 nm. From the analysis of the variation of the axial positions of the labels we concluded that the cellular surface was ruffled. To achieve sufficient stability of the sample under the electron beam irradiation during the recording of the focal series, the sample was carbon coated. A quantitative method was developed to analyze the stability of the ultrastructure after electron beam irradiation using TEM. The results of this study demonstrate the feasibility of using aberration-corrected STEM to study the 3D nanoparticle distribution in whole cells. PMID:21440635

Toward atomic-scale bright-field electron tomography for the study of fullerene-like nanostructures.

PubMed

Bar Sadan, Maya; Houben, Lothar; Wolf, Sharon G; Enyashin, Andrey; Seifert, Gotthard; Tenne, Reshef; Urban, Knut

2008-03-01

We present the advancement of electron tomography for three-dimensional structure reconstruction of fullerene-like particles toward atomic-scale resolution. The three-dimensional reconstruction of nested molybdenum disulfide nanooctahedra is achieved by the combination of low voltage operation of the electron microscope with aberration-corrected phase contrast imaging. The method enables the study of defects and irregularities in the three-dimensional structure of individual fullerene-like particles on the scale of 2-3 A. Control over shape, size, and atomic architecture is a key issue in synthesis and design of functional nanoparticles. Transmission electron microscopy (TEM) is the primary technique to characterize materials down to the atomic level, albeit the images are two-dimensional projections of the studied objects. Recent advancements in aberration-corrected TEM have demonstrated single atom sensitivity for light elements at subångström resolution. Yet, the resolution of tomographic schemes for three-dimensional structure reconstruction has not surpassed 1 nm3, preventing it from becoming a powerful tool for characterization in the physical sciences on the atomic scale. Here we demonstrate that negative spherical aberration imaging at low acceleration voltage enables tomography down to the atomic scale at reduced radiation damage. First experimental data on the three-dimensional reconstruction of nested molybdenum disulfide nanooctahedra is presented. The method is applicable to the analysis of the atomic architecture of a wide range of nanostructures where strong electron channeling is absent, in particular to carbon fullerenes and inorganic fullerenes.

Combining Monitoring Data Spanning Multiple Temporal and Spatial Scales To Evaluate Water Quality Affecting Seagrass Habitat Extent in northwest Florida Estuaries

EPA Science Inventory

The ability to understand and manage ecological changes caused by anthropogenic stressors is often impeded by a lack of sufficient information to resolve pattern and change with sufficient resolution and extent. Increasingly, different types of environmental data are available t...

Observation of the Quantum Hall Effect in Confined Films of the Three-Dimensional Dirac Semimetal Cd3 As2

NASA Astrophysics Data System (ADS)

Schumann, Timo; Galletti, Luca; Kealhofer, David A.; Kim, Honggyu; Goyal, Manik; Stemmer, Susanne

2018-01-01

The magnetotransport properties of epitaxial films of Cd3 As2 , a paradigm three-dimensional Dirac semimetal, are investigated. We show that an energy gap opens in the bulk electronic states of sufficiently thin films and, at low temperatures, carriers residing in surface states dominate the electrical transport. The carriers in these states are sufficiently mobile to give rise to a quantized Hall effect. The sharp quantization demonstrates surface transport that is virtually free of parasitic bulk conduction and paves the way for novel quantum transport studies in this class of topological materials. Our results also demonstrate that heterostructuring approaches can be used to study and engineer quantum states in topological semimetals.

Three-dimensional hydrogen microscopy using a high-energy proton probe

NASA Astrophysics Data System (ADS)

Dollinger, G.; Reichart, P.; Datzmann, G.; Hauptner, A.; Körner, H.-J.

2003-01-01

It is a challenge to measure two-dimensional or three-dimensional (3D) hydrogen profiles on a micrometer scale. Quantitative hydrogen analyses of micrometer resolution are demonstrated utilizing proton-proton scattering at a high-energy proton microprobe. It has more than an-order-of-magnitude better position resolution and in addition higher sensitivity than any other technique for 3D hydrogen analyses. This type of hydrogen imaging opens plenty room to characterize microstructured materials, and semiconductor devices or objects in microbiology. The first hydrogen image obtained with a 10 MeV proton microprobe shows the hydrogen distribution of the microcapillary system being present in the wing of a mayfly and demonstrates the potential of the method.

High-Resolution Gamma-Ray Imaging Measurements Using Externally Segmented Germanium Detectors

NASA Technical Reports Server (NTRS)

Callas, J.; Mahoney, W.; Skelton, R.; Varnell, L.; Wheaton, W.

1994-01-01

Fully two-dimensional gamma-ray imaging with simultaneous high-resolution spectroscopy has been demonstrated using an externally segmented germanium sensor. The system employs a single high-purity coaxial detector with its outer electrode segmented into 5 distinct charge collection regions and a lead coded aperture with a uniformly redundant array (URA) pattern. A series of one-dimensional responses was collected around 511 keV while the system was rotated in steps through 180 degrees. A non-negative, linear least-squares algorithm was then employed to reconstruct a 2-dimensional image. Corrections for multiple scattering in the detector, and the finite distance of source and detector are made in the reconstruction process.

Design considerations for ultra-precision magnetic bearing supported slides

NASA Technical Reports Server (NTRS)

Slocum, Alexander H.; Eisenhaure, David B.

1993-01-01

Development plans for a prototype servocontrolled machine with 1 angstrom resolution of linear motion and 50 mm range of travel are described. Two such devices could then be combined to produce a two dimensional machine for probing large planar objects with atomic resolution, the Angstrom Resolution Measuring Machine (ARMM).

Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals

DOE PAGES

Casadei, Cecilia M.; Tsai, Ching-Ju; Barty, Anton; ...

2018-01-01

Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4 Å. The results show that two-dimensional serial crystallography atmore » X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump–probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals.« less

Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Casadei, Cecilia M.; Tsai, Ching-Ju; Barty, Anton

Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4 Å. The results show that two-dimensional serial crystallography atmore » X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump–probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals.« less

Erratum: The Effects of Thermal Energetics on Three-dimensional Hydrodynamic Instabilities in Massive Protostellar Disks. II. High-Resolution and Adiabatic Evolutions

NASA Astrophysics Data System (ADS)

Pickett, Brian K.; Cassen, Patrick; Durisen, Richard H.; Link, Robert

2000-02-01

In the paper ``The Effects of Thermal Energetics on Three-dimensional Hydrodynamic Instabilities in Massive Protostellar Disks. II. High-Resolution and Adiabatic Evolutions'' by Brian K. Pickett, Patrick Cassen, Richard H. Durisen, and Robert Link (ApJ, 529, 1034 [2000]), the wrong version of Figure 10 was published as a result of an error at the Press. The correct version of Figure 10 appears below. The Press sincerely regrets this error.

Model studies of laser absorption computed tomography for remote air pollution measurement

NASA Technical Reports Server (NTRS)

Wolfe, D. C., Jr.; Byer, R. L.

1982-01-01

Model studies of the potential of laser absorption-computed tomography are presented which demonstrate the possibility of sensitive remote atmospheric pollutant measurements, over kilometer-sized areas, with two-dimensional resolution, at modest laser source powers. An analysis of this tomographic reconstruction process as a function of measurement SNR, laser power, range, and system geometry, shows that the system is able to yield two-dimensional maps of pollutant concentrations at ranges and resolutions superior to those attainable with existing, direct-detection laser radars.

Small-animal CT: Its difference from, and impact on, clinical CT

NASA Astrophysics Data System (ADS)

Ritman, Erik L.

2007-10-01

For whole-body computed tomography (CT) images of small rodents, a voxel resolution of at least 10 -3 mm 3 is needed for scale-equivalence to that currently achieved in clinical CT scanners (˜1 mm 3) in adult humans. These "mini-CT" images generally require minutes rather than seconds to complete a scan. The radiation exposure resulting from these mini-CT scans, while higher than clinical CT scans, is below the level resulting in acute tissue damage. Hence, these scans are useful for performing clinical-type diagnostic and monitoring scans for animal models of disease and their response to treatment. "Micro-CT", with voxel size <10 -5 mm 3, has been useful for imaging isolated, intact organs at an almost cellular level of resolution. Micro-CT has the great advantage over traditional microscopic methods in that it generates detailed three-dimensional images in relatively large, opaque volumes such as an intact rodent heart or kidney. The radiation exposure needed in these scans results in acute tissue damage if used in living animals. Experience with micro-CT is contributing to exploration of new applications for clinical CT imaging by providing insights into different modes of X-ray image formation as follows: Spatial resolution should be sufficient to detect an individual Basic Functional Unit (BFU, the smallest collection of diverse cells, such as hepatic lobule, that behaves like the organ), which requires voxels ˜10 -3 mm 3 in volume, so that the BFUs can be counted. Contrast resolution sufficient to allow quantitation of: New microvascular growth, which manifests as increased tissue contrast due to X-ray contrast agent in those vessels' lumens during passage of injected contrast agent in blood. Impaired endothelial integrity which manifests as increased opacification and delayed washout of contrast from tissues. Discrimination of pathological accumulations of metals such as Fe and Ca, which occur in the arterial wall following hemorrhage or tissue damage. Micro-CT can also be used as a test bed for exploring the utility of several modes of X-ray image formation, such as the use of dual-energy X-ray subtraction, X-ray scatter, phase delay and refraction-based imaging for increasing the contrast amongst soft tissue components. With the recent commercial availability of high speed, multi-slice CT scanners which can be operated in dual-energy mode, some of these micro-CT scanner capabilities and insights are becoming implementable in those CT scanners. As a result, the potential diagnostic spectrum that can be addressed with those scanners is broadened considerably.

Dimensional quantization effects in the thermodynamics of conductive filaments

NASA Astrophysics Data System (ADS)

Niraula, D.; Grice, C. R.; Karpov, V. G.

2018-06-01

We consider the physical effects of dimensional quantization in conductive filaments that underlie operations of some modern electronic devices. We show that, as a result of quantization, a sufficiently thin filament acquires a positive charge. Several applications of this finding include the host material polarization, the stability of filament constrictions, the equilibrium filament radius, polarity in device switching, and quantization of conductance.

Dimensional quantization effects in the thermodynamics of conductive filaments.

PubMed

Niraula, D; Grice, C R; Karpov, V G

2018-06-29

We consider the physical effects of dimensional quantization in conductive filaments that underlie operations of some modern electronic devices. We show that, as a result of quantization, a sufficiently thin filament acquires a positive charge. Several applications of this finding include the host material polarization, the stability of filament constrictions, the equilibrium filament radius, polarity in device switching, and quantization of conductance.

Deep and high-resolution three-dimensional tracking of single particles using nonlinear and multiplexed illumination

NASA Astrophysics Data System (ADS)

Perillo, Evan P.; Liu, Yen-Liang; Huynh, Khang; Liu, Cong; Chou, Chao-Kai; Hung, Mien-Chie; Yeh, Hsin-Chih; Dunn, Andrew K.

2015-07-01

Molecular trafficking within cells, tissues and engineered three-dimensional multicellular models is critical to the understanding of the development and treatment of various diseases including cancer. However, current tracking methods are either confined to two dimensions or limited to an interrogation depth of ~15 μm. Here we present a three-dimensional tracking method capable of quantifying rapid molecular transport dynamics in highly scattering environments at depths up to 200 μm. The system has a response time of 1 ms with a temporal resolution down to 50 μs in high signal-to-noise conditions, and a spatial localization precision as good as 35 nm. Built on spatiotemporally multiplexed two-photon excitation, this approach requires only one detector for three-dimensional particle tracking and allows for two-photon, multicolour imaging. Here we demonstrate three-dimensional tracking of epidermal growth factor receptor complexes at a depth of ~100 μm in tumour spheroids.

IAU resolutions on reference systems and time scales in practice

NASA Astrophysics Data System (ADS)

Brumberg, V. A.; Groten, E.

2001-03-01

To be consistent with IAU/IUGG (1991) resolutions ICRS and ITRS should be treated as four-dimensional reference systems with TCB and TCG time scales, respectively, interrelated by a four-dimensional general relativistic transformation. This two-way transformation is given in the form adapted for actual application. The use of TB and TT instead of TCB and TCG, respectively, involves scaling factors complicating the use of this transformation in practice. New IAU B1 (2000) resolution is commented taking in mind some points of possible confusion in its practical application. The problem of the relationship of the theory of reference systems with the parameters of common relevance to astronomy, geodesy and geodynamics is briefly outlined.

Wide-bandwidth, wide-beamwidth, high-resolution, millimeter-wave imaging for concealed weapon detection

NASA Astrophysics Data System (ADS)

Sheen, David M.; Fernandes, Justin L.; Tedeschi, Jonathan R.; McMakin, Douglas L.; Jones, A. Mark; Lechelt, Wayne M.; Severtsen, Ronald H.

2013-05-01

Active millimeter-wave imaging is currently being used for personnel screening at airports and other high-security facilities. The cylindrical imaging techniques used in the deployed systems are based on licensed technology developed at the Pacific Northwest National Laboratory. The cylindrical and a related planar imaging technique form three-dimensional images by scanning a diverging beam swept frequency transceiver over a two-dimensional aperture and mathematically focusing or reconstructing the data into three-dimensional images of the person being screened. The resolution, clothing penetration, and image illumination quality obtained with these techniques can be significantly enhanced through the selection of the aperture size, antenna beamwidth, center frequency, and bandwidth. The lateral resolution can be improved by increasing the center frequency, or it can be increased with a larger antenna beamwidth. The wide beamwidth approach can significantly improve illumination quality relative to a higher frequency system. Additionally, a wide antenna beamwidth allows for operation at a lower center frequency resulting in less scattering and attenuation from the clothing. The depth resolution of the system can be improved by increasing the bandwidth. Utilization of extremely wide bandwidths of up to 30 GHz can result in depth resolution as fine as 5 mm. This wider bandwidth operation may allow for improved detection techniques based on high range resolution. In this paper, the results of an extensive imaging study that explored the advantages of using extremely wide beamwidth and bandwidth are presented, primarily for 10-40 GHz frequency band.

Soft x-ray holographic tomography for biological specimens

NASA Astrophysics Data System (ADS)

Gao, Hongyi; Chen, Jianwen; Xie, Honglan; Li, Ruxin; Xu, Zhizhan; Jiang, Shiping; Zhang, Yuxuan

2003-10-01

In this paper, we present some experimental results on X -ray holography, holographic tomography, and a new holographic tomography method called pre-amplified holographic tomography is proposed. Due to the shorter wavelength and the larger penetration depths, X-rays provide the potential of higher resolution in imaging techniques, and have the ability to image intact, living, hydrated cells w ithout slicing, dehydration, chemical fixation or stain. Recently, using X-ray source in National Synchrotron Radiation Laboratory in Hefei, we have successfully performed some soft X-ray holography experiments on biological specimen. The specimens used in the experiments was the garlic clove epidermis, we got their X-ray hologram, and then reconstructed them by computer programs, the feature of the cell walls, the nuclei and some cytoplasm were clearly resolved. However, there still exist some problems in realization of practical 3D microscopic imaging due to the near-unity refractive index of the matter. There is no X-ray optics having a sufficient high numerical aperture to achieve a depth resolution that is comparable to the transverse resolution. On the other hand, computer tomography needs a record of hundreds of views of the test object at different angles for high resolution. This is because the number of views required for a densely packed object is equal to the object radius divided by the desired depth resolution. Clearly, it is impractical for a radiation-sensitive biological specimen. Moreover, the X-ray diffraction effect makes projection data blur, this badly degrades the resolution of the reconstructed image. In order to observe 3D structure of the biological specimens, McNulty proposed a new method for 3D imaging called "holographic tomography (HT)" in which several holograms of the specimen are recorded from various illumination directions and combined in the reconstruction step. This permits the specimens to be sampled over a wide range of spatial frequencies to improve the depth resolution. In NSRL, we performed soft X-ray holographic tomography experiments. The specimen was the spider filaments and PM M A as recording medium. By 3D CT reconstruction of the projection data, three dimensional density distribution of the specimen was obtained. Also, we developed a new X-ray holographic tomography m ethod called pre-amplified holographic tomography. The method permits a digital real-time 3D reconstruction with high-resolution and a simple and compact experimental setup as well.

Highly undersampled contrast-enhanced MRA with iterative reconstruction: Integration in a clinical setting.

PubMed

Stalder, Aurelien F; Schmidt, Michaela; Quick, Harald H; Schlamann, Marc; Maderwald, Stefan; Schmitt, Peter; Wang, Qiu; Nadar, Mariappan S; Zenge, Michael O

2015-12-01

To integrate, optimize, and evaluate a three-dimensional (3D) contrast-enhanced sparse MRA technique with iterative reconstruction on a standard clinical MR system. Data were acquired using a highly undersampled Cartesian spiral phyllotaxis sampling pattern and reconstructed directly on the MR system with an iterative SENSE technique. Undersampling, regularization, and number of iterations of the reconstruction were optimized and validated based on phantom experiments and patient data. Sparse MRA of the whole head (field of view: 265 × 232 × 179 mm(3) ) was investigated in 10 patient examinations. High-quality images with 30-fold undersampling, resulting in 0.7 mm isotropic resolution within 10 s acquisition, were obtained. After optimization of the regularization factor and of the number of iterations of the reconstruction, it was possible to reconstruct images with excellent quality within six minutes per 3D volume. Initial results of sparse contrast-enhanced MRA (CEMRA) in 10 patients demonstrated high-quality whole-head first-pass MRA for both the arterial and venous contrast phases. While sparse MRI techniques have not yet reached clinical routine, this study demonstrates the technical feasibility of high-quality sparse CEMRA of the whole head in a clinical setting. Sparse CEMRA has the potential to become a viable alternative where conventional CEMRA is too slow or does not provide sufficient spatial resolution. © 2014 Wiley Periodicals, Inc.

Filament organization revealed in platinum replicas of freeze-dried cytoskeletons

PubMed Central

1980-01-01

This report presents the appearance of rapidly frozen, freeze-dried cytoskeletons that have been rotary replicated with platinum and viewed in the transmission electron microscope. The resolution of this method is sufficient to visualize individual filaments in the cytoskeleton and to discriminate among actin, microtubules, and intermediate filaments solely by their surface substructure. This identification has been confirmed by specific decoration with antibodies and selective extraction of individual filament types, and correlated with light microscope immunocytochemistry and gel electrophoresis patterns. The freeze-drying preserves a remarkable degree of three-dimensionality in the organization of these cytoskeletons. They look strikingly similar to the meshwork of strands or "microtrabeculae" seen in the cytoplasm of whole cells by high voltage electron microscopy, in that the filaments form a lattice of the same configutation and with the same proportions of open area as the microtrabeculae seen in whole cells. The major differences between these two views of the structural elements of the cytoplasmic matrix can be attributed to the effects of aldehyde fixation and dehydration. Freeze-dried cytoskeletons thus provide an opportunity to study--at high resolution and in the absence of problems caused by chemical fixation--the detailed organization of filaments in different regions of the cytoplasm and at different stages of cell development. In this report the pattern of actin and intermediate filament organization in various regions of fully spread mouse fibroblasts is described. PMID:6893451

ON GALACTIC DENSITY MODELING IN THE PRESENCE OF DUST EXTINCTION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bovy, Jo; Rix, Hans-Walter; Schlafly, Edward F.

Inferences about the spatial density or phase-space structure of stellar populations in the Milky Way require a precise determination of the effective survey volume. The volume observed by surveys such as Gaia or near-infrared spectroscopic surveys, which have good coverage of the Galactic midplane region, is highly complex because of the abundant small-scale structure in the three-dimensional interstellar dust extinction. We introduce a novel framework for analyzing the importance of small-scale structure in the extinction. This formalism demonstrates that the spatially complex effect of extinction on the selection function of a pencil-beam or contiguous sky survey is equivalent to amore » low-pass filtering of the extinction-affected selection function with the smooth density field. We find that the angular resolution of current 3D extinction maps is sufficient for analyzing Gaia sub-samples of millions of stars. However, the current distance resolution is inadequate and needs to be improved by an order of magnitude, especially in the inner Galaxy. We also present a practical and efficient method for properly taking the effect of extinction into account in analyses of Galactic structure through an effective selection function. We illustrate its use with the selection function of red-clump stars in APOGEE using and comparing a variety of current 3D extinction maps.« less

Kirkpatrick-Baez microscope for hard X-ray imaging of fast ignition experiments.

PubMed

Friesen, H; Tiedje, H F; Hey, D S; Mo, M Z; Beaudry, A; Fedosejevs, R; Tsui, Y Y; Mackinnon, A; McLean, H S; Patel, P K

2013-02-01

A Kirkpatrick-Baez X-ray microscope has been developed for use on the Titan laser facility at the Lawrence Livermore National Laboratory in Fast Ignition experiments. It was developed as a broadband alternative to narrow band Bragg crystal imagers for imaging Kα emission from tracer layers. A re-entrant design is employed which allows for alignment from outside the chamber. The mirrors are coated with Pt and operate at a grazing incident angle of 0.5° providing higher resolution than an equal brightness pinhole and sufficient bandwidth to image thermally shifted characteristic Kα emission from heated Cu tracer layers in Fast Ignition experiments. The superpolished substrates (<1 Å rms roughness) had a final visible wavelength roughness of 1.7 Å after coating, and exhibited a reflectivity corresponding to an X-ray wavelength roughness of 7 ± 1 Å. A unique feature of this design is that during experiments, the unfiltered direct signal along with the one-dimensional reflections are retained on the detector in order to enable a live indication of alignment and incident angle. The broad spectral window from 4 to 9 keV enables simultaneous observation of emission from several spectral regions of interest, which has been demonstrated to be particularly useful for cone-wire targets. An experimentally measured resolution of 15 μm has been obtained at the center of the field of view.

Spatially-resolved temperature diagnostic for supersonic flow using cross-beam Doppler-limited laser saturation spectroscopy

NASA Astrophysics Data System (ADS)

Phillips, Grady T.

Optical techniques for measuring the temperature in three-dimensional supersonic reactive flows have typically depended on lineshape measurements using single-beam laser absorption spectroscopy. However, absorption over extended path lengths in flows with symmetric, turbulent eddies can lead to systematically high extracted temperatures due to Doppler shifts resulting from flow along the absorption path. To eliminate these problems and provide full three-dimensional spatial resolution, two variants of laser saturation spectroscopy have been developed and demonstrated, for the first time, which utilize two crossed and nearly copropogating laser beams. Individual rotational lines in the visible I2 X 1Sigma 0+g → B 3pi 0+u transition were used to develop the two diagnostic to support research on the Chemical Oxygen-Iodine Laser (COIL), the weapon aboard the USAF Airborne Laser. Cross-Beam Saturation Absorption Spectroscopy (CBSAS) and Cross-Beam Inter-Modulated Fluorescence (CBIMF) were demonstrated as viable methods for recording the spectral signal of an I2 ro-vibrational line in a small three-dimensional volume using a tunable CW dye laser. Temperature is extracted by fitting the recorded signal with a theoretical signal constructed from the Doppler-broadened hyperfine components of the ro-vibrational line. The CBIMF technique proved successful for extracting the temperature of an I2-seeded, Ar gas flow within a small, Mach 2, Laval nozzle where the overlap volume of the two 1 mm diameter laser beams was 2.4 mm 3. At a test point downstream of the nozzle throat, the average temperature of 146 K +/- 1.5 K extracted from measurements of the I2 P(46) 17-1 spectral line compared favorably with the 138 K temperature calculated from isentropic, one-dimensional flow theory. CBIMF provides sufficient accuracy for characterizing the temperature of the gas flow in a COIL device, and could be applied to other areas of flow-field characterization and nozzle design. In contrast, the CBSAS signal was not sufficiently strong for reliable temperature extraction from the 2.4 mm3 overlap volume required in the nozzle experiments. Otherwise, the CBSAS technique could have greater success for application in flow field test environments that allow the use of a larger overlap-volume. CBIMF and CBSAS measurements were also made in a static cell at 293 K. At 50 mTorr of I2, the standard error in temperature from CBIMF measurements of the I2 P(46) 17-1 line was approximately 0.5 K. For CBSAS, the standard error in temperature was approximately 3 K at 50 mTorr of I2. Accuracy improved with increasing I2 pressure. In addition, the spatial-resolution capability of CBIMF and CBSAS was demonstrated in a static cell with an applied temperature gradient ranging from 300 to 365 K. Extracted temperatures were compared to thermocouple measurements at multiple positions in the gradient. Agreement between extracted temperatures and thermocouple measurements was better at the lower temperatures. Doppler-free measurements of several I2 hyperfine spectra were also performed to support development of the theoretical model. Saturation Absorption Spectroscopy was used to obtain Ar pressure broadening rates of 8.29 +/- 0.30 MHz/Torr for the I2 P(70) 17-1 hyperfine spectrum, and 10.70 +/- 0.41 MHz/Torr for the I2 P(10) 17-1 hyperfine spectrum.

Evaluation of shoulder pathology: three-dimensional enhanced T1 high-resolution isotropic volume excitation MR vs two-dimensional fast spin echo T2 fat saturation MR.

PubMed

Park, H J; Lee, S Y; Kim, M S; Choi, S H; Chung, E C; Kook, S H; Kim, E

2015-03-01

To evaluate the diagnostic accuracy of three-dimensional (3D) enhanced T1 high-resolution isotropic volume excitation (eTHRIVE) shoulder MR for the detection of rotator cuff tears, labral lesions and calcific tendonitis of the rotator cuff in comparison with two-dimensional (2D) fast spin echo T2 fat saturation (FS) MR. This retrospective study included 73 patients who underwent shoulder MRI using the eTHRIVE technique. Shoulder MR images were interpreted separately by two radiologists. They evaluated anatomic identification and image quality of the shoulder joint on routine MRI sequences (axial and oblique coronal T2 FS images) and compared them with the reformatted eTHRIVE images. The images were scored on a four-point scale (0, poor; 1, questionable; 2, adequate; 3, excellent) according to the degree of homogeneous and sufficient fat saturation to penetrate bone and soft tissue, visualization of the glenoid labrum and distinction of the supraspinatus tendon (SST). The diagnostic accuracy of eTHRIVE images compared with routine MRI sequences was evaluated in the setting of rotator cuff tears, glenoid labral injuries and calcific tendonitis of the SST. Fat saturation scores for eTHRIVE were significantly higher than those of the T2 FS for both radiologists. The sensitivity and accuracy of the T2 FS in diagnosing rotor cuff tears were >90%, whereas sensitivity and accuracy of the eTHRIVE method were significantly lower. The sensitivity, specificity and accuracy of both images in diagnosing labral injuries and calcific tendonitis were similar and showed no significant differences. The specificity of both images for the diagnosis of labral injuries and calcific tendonitis was higher than the sensitivities. The accuracy of 3D eTHRIVE imaging was comparable to that of 2D FSE T2 FS for the diagnosis of glenoid labral injury and calcific tendonitis of SST. The 3D eTHRIVE technique was superior to 2D FSE T2 FS in terms of fat saturation. Overall, 3D eTHRIVE was inferior to T2 FS in the evaluation of rotator cuff tears because of poor contrast between joint fluid and tendons. The accuracy of 3D eTHRIVE imaging is comparable to that of 2D FSE T2 FS for the diagnosis of glenoid labral injury and calcific tendonitis of SST.

Rapid high-resolution four-dimensional NMR spectroscopy using the filter diagonalization method and its advantages for detailed structural elucidation of oligosaccharides.

PubMed

Armstrong, Geoffrey S; Mandelshtam, Vladimir A; Shaka, A J; Bendiak, Brad

2005-03-01

Four-dimensional nuclear magnetic resonance spectroscopy with high resolution of signals in the indirect dimensions is reported as an implementation of the filter diagonalization method (FDM). Using an oligosaccharide derivatized with 13C-labeled acetyl isotags, a four-dimensional constant-time pulse sequence was tailored for conjoint use with the FDM. Results demonstrate that high resolution in all dimensions can be achieved using a relatively short experimental time period (19 h), even though the spectrum is highly congested in the direct and all three indirect dimensions. The combined use of isotags, constant-time pulse sequences, and FDM permits rapid isolation of sugar ring proton spin systems in multiple dimensions and enables all endocyclic J-couplings to be simply measured, the key goal to assigning sugar stereochemistry and anomeric configuration. A general method for rapid, unambiguous elucidation of spin systems in oligosaccharides has been a long-sought goal of carbohydrate NMR, and isotags combined with the FDM now enable this to be easily performed. Additional general advantages of the FDM program for generating high-resolution 2D slices in any dimension from a 4D spectrum are emphasized.

Retinal Optical Coherence Tomography Imaging

NASA Astrophysics Data System (ADS)

Drexler, Wolfgang; Fujimoto, James G.

The eye is essentially transparent, transmitting light with only minimal optical attenuation and scattering providing easy optical access to the anterior segment as well as the retina. For this reason, ophthalmic and especially retinal imaging has been not only the first but also most successful clinical application for optical coherence tomography (OCT). This chapter focuses on the development of OCT technology for retinal imaging. OCT has significantly improved the potential for early diagnosis, understanding of retinal disease pathogenesis, as well as monitoring disease progression and response to therapy. Development of ultrabroad bandwidth light sources and high-speed detection techniques has enabled significant improvements in ophthalmic OCT imaging performance, demonstrating the potential of three-dimensional, ultrahigh-resolution OCT (UHR OCT) to perform noninvasive optical biopsy of the living human retina, i.e., the in vivo visualization of microstructural, intraretinal morphology in situ approaching the resolution of conventional histopathology. Significant improvements in axial resolution and speed not only enable three-dimensional rendering of retinal volumes but also high-definition, two-dimensional tomograms, topographic thickness maps of all major intraretinal layers, as well as volumetric quantification of pathologic intraretinal changes. These advances in OCT technology have also been successfully applied in several animal models of retinal pathologies. The development of light sources emitting at alternative wavelengths, e.g., around #1,060 nm, not only enabled three-dimensional OCT imaging with enhanced choroidal visualization but also improved OCT performance in cataract patients due to reduced scattering losses in this wavelength region. Adaptive optics using deformable mirror technology, with unique high stroke to correct higher-order ocular aberrations, with specially designed optics to compensate chromatic aberration of the human eye, in combination with three-dimensional UHR OCT, recently enabled in vivo cellular resolution retinal imaging.

Three-dimensional characterization of bacterial microcolonies on solid agar-based culture media.

PubMed

Drazek, Laurent; Tournoud, Maud; Derepas, Frédéric; Guicherd, Maryse; Mahé, Pierre; Pinston, Frédéric; Veyrieras, Jean-Baptiste; Chatellier, Sonia

2015-02-01

For the last century, in vitro diagnostic process in microbiology has mainly relied on the growth of bacteria on the surface of a solid agar medium. Nevertheless, few studies focused in the past on the dynamics of microcolonies growth on agar surface before 8 to 10h of incubation. In this article, chromatic confocal microscopy has been applied to characterize the early development of a bacterial colony. This technology relies on a differential focusing depth of the white light. It allows one to fully measure the tridimensional shape of microcolonies more quickly than classical confocal microscopy but with the same spatial resolution. Placing the device in an incubator, the method was able to individually track colonies growing on an agar plate, and to follow the evolution of their surface or volume. Using an appropriate statistical modeling framework, for a given microorganism, the doubling time has been estimated for each individual colony, as well as its variability between colonies, both within and between agar plates. A proof of concept led on four bacterial strains of four distinct species demonstrated the feasibility and the interest of the approach. It showed in particular that doubling times derived from early tri-dimensional measurements on microcolonies differed from classical measurements in micro-dilutions based on optical diffusion. Such a precise characterization of the tri-dimensional shape of microcolonies in their late-lag to early-exponential phase could be beneficial in terms of in vitro diagnostics. Indeed, real-time monitoring of the biomass available in a colony could allow to run well established microbial identification workflows like, for instance, MALDI-TOF mass-spectrometry, as soon as a sufficient quantity of material is available, thereby reducing the time needed to provide a diagnostic. Moreover, as done for pre-identification of macro-colonies, morphological indicators such as three-dimensional growth profiles derived from microcolonies could be used to perform a first pre-identification step, but in a shorten time. Copyright © 2014 Elsevier B.V. All rights reserved.

Multi-Resolution Climate Ensemble Parameter Analysis with Nested Parallel Coordinates Plots.

PubMed

Wang, Junpeng; Liu, Xiaotong; Shen, Han-Wei; Lin, Guang

2017-01-01

Due to the uncertain nature of weather prediction, climate simulations are usually performed multiple times with different spatial resolutions. The outputs of simulations are multi-resolution spatial temporal ensembles. Each simulation run uses a unique set of values for multiple convective parameters. Distinct parameter settings from different simulation runs in different resolutions constitute a multi-resolution high-dimensional parameter space. Understanding the correlation between the different convective parameters, and establishing a connection between the parameter settings and the ensemble outputs are crucial to domain scientists. The multi-resolution high-dimensional parameter space, however, presents a unique challenge to the existing correlation visualization techniques. We present Nested Parallel Coordinates Plot (NPCP), a new type of parallel coordinates plots that enables visualization of intra-resolution and inter-resolution parameter correlations. With flexible user control, NPCP integrates superimposition, juxtaposition and explicit encodings in a single view for comparative data visualization and analysis. We develop an integrated visual analytics system to help domain scientists understand the connection between multi-resolution convective parameters and the large spatial temporal ensembles. Our system presents intricate climate ensembles with a comprehensive overview and on-demand geographic details. We demonstrate NPCP, along with the climate ensemble visualization system, based on real-world use-cases from our collaborators in computational and predictive science.

Two dimensional molecular electronics spectroscopy for molecular fingerprinting, DNA sequencing, and cancerous DNA recognition.

PubMed

Rajan, Arunkumar Chitteth; Rezapour, Mohammad Reza; Yun, Jeonghun; Cho, Yeonchoo; Cho, Woo Jong; Min, Seung Kyu; Lee, Geunsik; Kim, Kwang S

2014-02-25

Laser-driven molecular spectroscopy of low spatial resolution is widely used, while electronic current-driven molecular spectroscopy of atomic scale resolution has been limited because currents provide only minimal information. However, electron transmission of a graphene nanoribbon on which a molecule is adsorbed shows molecular fingerprints of Fano resonances, i.e., characteristic features of frontier orbitals and conformations of physisorbed molecules. Utilizing these resonance profiles, here we demonstrate two-dimensional molecular electronics spectroscopy (2D MES). The differential conductance with respect to bias and gate voltages not only distinguishes different types of nucleobases for DNA sequencing but also recognizes methylated nucleobases which could be related to cancerous cell growth. This 2D MES could open an exciting field to recognize single molecule signatures at atomic resolution. The advantages of the 2D MES over the one-dimensional (1D) current analysis can be comparable to those of 2D NMR over 1D NMR analysis.

3D imaging of cement-based materials at submicron resolution by combining laser scanning confocal microscopy with serial sectioning.

PubMed

Yio, M H N; Mac, M J; Wong, H S; Buenfeld, N R

2015-05-01

In this paper, we present a new method to reconstruct large volumes of nontransparent porous materials at submicron resolution. The proposed method combines fluorescence laser scanning confocal microscopy with serial sectioning to produce a series of overlapping confocal z-stacks, which are then aligned and stitched based on phase correlation. The method can be extended in the XY plane to further increase the overall image volume. Resolution of the reconstructed image volume does not degrade with increase in sample size. We have used the method to image cementitious materials, hardened cement paste and concrete and the results obtained show that the method is reliable. Possible applications of the method such as three-dimensional characterization of the pores and microcracks in hardened concrete, three-dimensional particle shape characterization of cementitious materials and three-dimensional characterization of other porous materials such as rocks and bioceramics are discussed. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

Two-dimensional Imaging Velocity Interferometry: Technique and Data Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Erskine, D J; Smith, R F; Bolme, C

2011-03-23

We describe the data analysis procedures for an emerging interferometric technique for measuring motion across a two-dimensional image at a moment in time, i.e. a snapshot 2d-VISAR. Velocity interferometers (VISAR) measuring target motion to high precision have been an important diagnostic in shockwave physics for many years Until recently, this diagnostic has been limited to measuring motion at points or lines across a target. We introduce an emerging interferometric technique for measuring motion across a two-dimensional image, which could be called a snapshot 2d-VISAR. If a sufficiently fast movie camera technology existed, it could be placed behind a traditional VISARmore » optical system and record a 2d image vs time. But since that technology is not yet available, we use a CCD detector to record a single 2d image, with the pulsed nature of the illumination providing the time resolution. Consequently, since we are using pulsed illumination having a coherence length shorter than the VISAR interferometer delay ({approx}0.1 ns), we must use the white light velocimetry configuration to produce fringes with significant visibility. In this scheme, two interferometers (illuminating, detecting) having nearly identical delays are used in series, with one before the target and one after. This produces fringes with at most 50% visibility, but otherwise has the same fringe shift per target motion of a traditional VISAR. The 2d-VISAR observes a new world of information about shock behavior not readily accessible by traditional point or 1d-VISARS, simultaneously providing both a velocity map and an 'ordinary' snapshot photograph of the target. The 2d-VISAR has been used to observe nonuniformities in NIF related targets (polycrystalline diamond, Be), and in Si and Al.« less

Quantifying the eroded volume of mercury-contaminated sediment using terrestrial laser scanning at Stocking Flat, Deer Creek, Nevada County, California, 2010–13

USGS Publications Warehouse

Howle, James F.; Alpers, Charles N.; Bawden, Gerald W.; Bond, Sandra

2016-07-28

High-resolution ground-based light detection and ranging (lidar), also known as terrestrial laser scanning, was used to quantify the volume of mercury-contaminated sediment eroded from a stream cutbank at Stocking Flat along Deer Creek in the Sierra Nevada foothills, about 3 kilometers west of Nevada City, California. Terrestrial laser scanning was used to collect sub-centimeter, three-dimensional images of the complex cutbank surface, which could not be mapped non-destructively or in sufficient detail with traditional surveying techniques.The stream cutbank, which is approximately 50 meters long and 8 meters high, was surveyed on four occasions: December 1, 2010; January 20, 2011; May 12, 2011; and February 4, 2013. Volumetric changes were determined between the sequential, three-dimensional lidar surveys. Volume was calculated by two methods, and the average value is reported. Between the first and second surveys (December 1, 2010, to January 20, 2011), a volume of 143 plus or minus 15 cubic meters of sediment was eroded from the cutbank and mobilized by Deer Creek. Between the second and third surveys (January 20, 2011, to May 12, 2011), a volume of 207 plus or minus 24 cubic meters of sediment was eroded from the cutbank and mobilized by the stream. Total volumetric change during the winter and spring of 2010–11 was 350 plus or minus 28 cubic meters. Between the third and fourth surveys (May 12, 2011, to February 4, 2013), the differencing of the three-dimensional lidar data indicated that a volume of 18 plus or minus 10 cubic meters of sediment was eroded from the cutbank. The total volume of sediment eroded from the cutbank between the first and fourth surveys was 368 plus or minus 30 cubic meters.

Applying a two-dimensional morphodynamic model to assess impacts to Chinook salmon spawning habitat from dam removal

NASA Astrophysics Data System (ADS)

Lee, A. A.; Crosato, A.; Omer, A. Y. A.; Bregoli, F.

2017-12-01

The need for accurate and robust predictive methods of assessing fluvial ecosystems is highlighted by the accelerating practice of dam removal. Dam removal can be a restorative measure, but the sudden release of impounded sediment and change in flow regime may negatively impact aquatic biota and their habitat. This study assesses the performance of a quasi-three-dimensional morphodynamic numerical model, coupled with habitat suitability indices, to predict short-term impacts to Chinook salmon (Oncorhynchus tshawytscha) spawning habitat from dam removal. The 2007 removal of Marmot Dam on the Sandy River (Oregon, U.S.A.) is used as a case study. Delft3D-FLOW is employed to simulate changes in river channel topography, sediment composition and hydrodynamic conditions for a 20-kilometer reach of the Sandy River. The transport of non-uniform sediment and three-dimensional flow effects are included in the model. Output parameters such as flow depth, velocity and substrate are processed to evaluate habitat quality in the year following the Marmot Dam removal. Impacts are evaluated across four life-stages of Chinook salmon. As a hindcast analysis, the morphodynamic model sufficiently reproduces the evolution of river morphology at the reach-scale while requiring only a low level of calibration. The model performs well in predicting impacts to fish passage, but carries more uncertainty for developing life stages. By coupling flow-sediment-biota interactions, this method shows strong potential for habitat assessment in unsteady and non-uniform environments. Computation time is a primary constraint, as it limits grid-cell resolution, modelling of suspended sediment and capacity to characterize the sediment grain size distribution. Research on the effects of suspended sediment on habitat quality is ongoing, and further research is recommended for modelling reservoir erosion processes numerically.

THREE-DIMENSIONAL MODEL FOR HYPERTHERMIA CALCULATIONS

EPA Science Inventory

Realistic three-dimensional models that predict temperature distributions with a high degree of spatial resolution in bodies exposed to electromagnetic (EM) fields are required in the application of hyperthermia for cancer treatment. To ascertain the thermophysiologic response of...

High Resolution Global Topography of Eros from NEAR Imaging and LIDAR Data

NASA Technical Reports Server (NTRS)

Gaskell, Robert W.; Konopliv, A.; Barnouin-Jha, O.; Scheeres, D.

2006-01-01

Principal Data Products: Ensemble of L-maps from SPC, Spacecraft state, Asteroid pole and rotation. Secondary Products: Global topography model, inertia tensor, gravity. Composite high resolution topography. Three dimensional image maps.

Biological imaging by soft X-ray diffraction microscopy

NASA Astrophysics Data System (ADS)

Shapiro, David

We have developed a microscope for soft x-ray diffraction imaging of dry or frozen hydrated biological specimens. This lensless imaging system does not suffer from the resolution or specimen thickness limitations that other short wavelength microscopes experience. The microscope, currently situated at beamline 9.0.1 of the Advanced Light Source, can collect diffraction data to 12 nm resolution with 750 eV photons and 17 nm resolution with 520 eV photons. The specimen can be rotated with a precision goniometer through an angle of 160 degrees allowing for the collection of nearly complete three-dimensional diffraction data. The microscope is fully computer controlled through a graphical user interface and a scripting language automates the collection of both two-dimensional and three-dimensional data. Diffraction data from a freeze-dried dwarf yeast cell, Saccharomyces cerevisiae carrying the CLN3-1 mutation, was collected to 12 run resolution from 8 specimen orientations spanning a total rotation of 8 degrees. The diffraction data was phased using the difference map algorithm and the reconstructions provide real space images of the cell to 30 nm resolution from each of the orientations. The agreement of the different reconstructions provides confidence in the recovered, and previously unknown, structure and indicates the three dimensionality of the cell. This work represents the first imaging of the natural complex refractive contrast from a whole unstained cell by the diffraction microscopy method and has achieved a resolution superior to lens based x-ray tomographic reconstructions of similar specimens. Studies of the effects of exposure to large radiation doses were also carried out. It was determined that the freeze-dried cell suffers from an initial collapse, which is followed by a uniform, but slow, shrinkage. This structural damage to the cell is not accompanied by a diminished ability to see small features in the specimen. Preliminary measurements on frozen-hydrated yeast indicate that the frozen specimens do not exhibit these changes even with doses as high as 5 x 109 Gray.

New functionalities of potassium tantalate niobate deflectors enabled by the coexistence of pre-injected space charge and composition gradient

NASA Astrophysics Data System (ADS)

Zhu, Wenbin; Chao, Ju-Hung; Chen, Chang-Jiang; Campbell, Adrian L.; Henry, Michael G.; Yin, Stuart Shizhuo; Hoffman, Robert C.

2017-10-01

In most beam steering applications such as 3D printing and in vivo imaging, one of the essential challenges has been high-resolution high-speed multi-dimensional optical beam scanning. Although the pre-injected space charge controlled potassium tantalate niobate (KTN) deflectors can achieve speeds in the nanosecond regime, they deflect in only one dimension. In order to develop a high-resolution high-speed multi-dimensional KTN deflector, we studied the deflection behavior of KTN deflectors in the case of coexisting pre-injected space charge and composition gradient. We find that such coexistence can enable new functionalities of KTN crystal based electro-optic deflectors. When the direction of the composition gradient is parallel to the direction of the external electric field, the zero-deflection position can be shifted, which can reduce the internal electric field induced beam distortion, and thus enhance the resolution. When the direction of the composition gradient is perpendicular to the direction of the external electric field, two-dimensional beam scanning can be achieved by harnessing only one single piece of KTN crystal, which can result in a compact, high-speed two-dimensional deflector. Both theoretical analyses and experiments are conducted, which are consistent with each other. These new functionalities can expedite the usage of KTN deflection in many applications such as high-speed 3D printing, high-speed, high-resolution imaging, and free space broadband optical communication.

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM).

PubMed

Wang, Yilin; Kanchanawong, Pakorn

2016-12-01

Fluorescence microscopy enables direct visualization of specific biomolecules within cells. However, for conventional fluorescence microscopy, the spatial resolution is restricted by diffraction to ~ 200 nm within the image plane and > 500 nm along the optical axis. As a result, fluorescence microscopy has long been severely limited in the observation of ultrastructural features within cells. The recent development of super resolution microscopy methods has overcome this limitation. In particular, the advent of photoswitchable fluorophores enables localization-based super resolution microscopy, which provides resolving power approaching the molecular-length scale. Here, we describe the application of a three-dimensional super resolution microscopy method based on single-molecule localization microscopy and multiphase interferometry, called interferometric PhotoActivated Localization Microscopy (iPALM). This method provides nearly isotropic resolution on the order of 20 nm in all three dimensions. Protocols for visualizing the filamentous actin cytoskeleton, including specimen preparation and operation of the iPALM instrument, are described here. These protocols are also readily adaptable and instructive for the study of other ultrastructural features in cells.

Resolution of co-eluting compounds of Cannabis Sativa in comprehensive two-dimensional gas chromatography/mass spectrometry detection with Multivariate Curve Resolution-Alternating Least Squares.

PubMed

Omar, Jone; Olivares, Maitane; Amigo, José Manuel; Etxebarria, Nestor

2014-04-01

Comprehensive Two Dimensional Gas Chromatography - Mass Spectrometry (GC × GC/qMS) analysis of Cannabis sativa extracts shows a high complexity due to the large variety of terpenes and cannabinoids and to the fact that the complete resolution of the peaks is not straightforwardly achieved. In order to support the resolution of the co-eluted peaks in the sesquiterpene and the cannabinoid chromatographic region the combination of Multivariate Curve Resolution and Alternating Least Squares algorithms was satisfactorily applied. As a result, four co-eluting areas were totally resolved in the sesquiterpene region and one in the cannabinoid region in different samples of Cannabis sativa. The comparison of the mass spectral profiles obtained for each resolved peak with theoretical mass spectra allowed the identification of some of the co-eluted peaks. Finally, the classification of the studied samples was achieved based on the relative concentrations of the resolved peaks. Copyright © 2014 Elsevier B.V. All rights reserved.

Maximizing the Biochemical Resolving Power of Fluorescence Microscopy

PubMed Central

Esposito, Alessandro; Popleteeva, Marina; Venkitaraman, Ashok R.

2013-01-01

Most recent advances in fluorescence microscopy have focused on achieving spatial resolutions below the diffraction limit. However, the inherent capability of fluorescence microscopy to non-invasively resolve different biochemical or physical environments in biological samples has not yet been formally described, because an adequate and general theoretical framework is lacking. Here, we develop a mathematical characterization of the biochemical resolution in fluorescence detection with Fisher information analysis. To improve the precision and the resolution of quantitative imaging methods, we demonstrate strategies for the optimization of fluorescence lifetime, fluorescence anisotropy and hyperspectral detection, as well as different multi-dimensional techniques. We describe optimized imaging protocols, provide optimization algorithms and describe precision and resolving power in biochemical imaging thanks to the analysis of the general properties of Fisher information in fluorescence detection. These strategies enable the optimal use of the information content available within the limited photon-budget typically available in fluorescence microscopy. This theoretical foundation leads to a generalized strategy for the optimization of multi-dimensional optical detection, and demonstrates how the parallel detection of all properties of fluorescence can maximize the biochemical resolving power of fluorescence microscopy, an approach we term Hyper Dimensional Imaging Microscopy (HDIM). Our work provides a theoretical framework for the description of the biochemical resolution in fluorescence microscopy, irrespective of spatial resolution, and for the development of a new class of microscopes that exploit multi-parametric detection systems. PMID:24204821

High-resolution absolute position detection using a multiple grating

NASA Astrophysics Data System (ADS)

Schilling, Ulrich; Drabarek, Pawel; Kuehnle, Goetz; Tiziani, Hans J.

1996-08-01

To control electro-mechanical engines, high-resolution linear and rotary encoders are needed. Interferometric methods (grating interferometers) promise a resolution of a few nanometers, but have an ambiguity range of some microns. Incremental encoders increase the absolute measurement range by counting the signal periods starting from a defined initial point. In many applications, however, it is not possible to move to this initial point, so that absolute encoders have to be used. Absolute encoders generally have a scale with two or more tracks placed next to each other. Therefore, they use a two-dimensional grating structure to measure a one-dimensional position. We present a new method, which uses a one-dimensional structure to determine the position in one dimension. It is based on a grating with a large grating period up to some millimeters, having the same diffraction efficiency in several predefined diffraction orders (multiple grating). By combining the phase signals of the different diffraction orders, it is possible to establish the position in an absolute range of the grating period with a resolution like incremental grating interferometers. The principal functionality was demonstrated by applying the multiple grating in a heterodyne grating interferometer. The heterodyne frequency was generated by a frequency modulated laser in an unbalanced interferometer. In experimental measurements an absolute range of 8 mm was obtained while achieving a resolution of 10 nm.

Phase space interrogation of the empirical response modes for seismically excited structures

NASA Astrophysics Data System (ADS)

Paul, Bibhas; George, Riya C.; Mishra, Sudib K.

2017-07-01

Conventional Phase Space Interrogation (PSI) for structural damage assessment relies on exciting the structure with low dimensional chaotic waveform, thereby, significantly limiting their applicability to large structures. The PSI technique is presently extended for structure subjected to seismic excitations. The high dimensionality of the phase space for seismic response(s) are overcome by the Empirical Mode Decomposition (EMD), decomposing the responses to a number of intrinsic low dimensional oscillatory modes, referred as Intrinsic Mode Functions (IMFs). Along with their low dimensionality, a few IMFs, retain sufficient information of the system dynamics to reflect the damage induced changes. The mutually conflicting nature of low-dimensionality and the sufficiency of dynamic information are taken care by the optimal choice of the IMF(s), which is shown to be the third/fourth IMFs. The optimal IMF(s) are employed for the reconstruction of the Phase space attractor following Taken's embedding theorem. The widely referred Changes in Phase Space Topology (CPST) feature is then employed on these Phase portrait(s) to derive the damage sensitive feature, referred as the CPST of the IMFs (CPST-IMF). The legitimacy of the CPST-IMF is established as a damage sensitive feature by assessing its variation with a number of damage scenarios benchmarked in the IASC-ASCE building. The damage localization capability, remarkable tolerance to noise contamination and the robustness under different seismic excitations of the feature are demonstrated.

The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5 Å resolution

PubMed Central

Moroz, Olga V.; Maranta, Michelle; Shaghasi, Tarana; Harris, Paul V.; Wilson, Keith S.; Davies, Gideon J.

2015-01-01

The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three-dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide-bound forms at resolutions of 1.8 and 1.5 Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts. PMID:25615982

Application of a transonic similarity rule to correct the effects of sidewall boundary layers in two-dimensional transonic wind tunnels. M.S. Thesis - George Washington Univ.

NASA Technical Reports Server (NTRS)

Sewall, W. G.

1982-01-01

A transonic similarity rule which accounts for the effects of attached sidewall boundary layers is presented and evaluated by comparison with the characteristics of airfoils tested in a two dimensional transonic tunnel with different sidewall boundary layer thicknesses. The rule appears valid provided the sidewall boundary layer both remains attached in the vicinity of the model and occupies a small enough fraction of the tunnel width to preserve sufficient two dimensionality in the tunnel.

Finite-Dimensional Representations for Controlled Diffusions with Delay

DOE Office of Scientific and Technical Information (OSTI.GOV)

Federico, Salvatore, E-mail: salvatore.federico@unimi.it; Tankov, Peter, E-mail: tankov@math.univ-paris-diderot.fr

2015-02-15

We study stochastic delay differential equations (SDDE) where the coefficients depend on the moving averages of the state process. As a first contribution, we provide sufficient conditions under which the solution of the SDDE and a linear path functional of it admit a finite-dimensional Markovian representation. As a second contribution, we show how approximate finite-dimensional Markovian representations may be constructed when these conditions are not satisfied, and provide an estimate of the error corresponding to these approximations. These results are applied to optimal control and optimal stopping problems for stochastic systems with delay.

Structural Properties and Estimation of Delay Systems. Ph.D. Thesis

NASA Technical Reports Server (NTRS)

Kwong, R. H. S.

1975-01-01

Two areas in the theory of delay systems were studied: structural properties and their applications to feedback control, and optimal linear and nonlinear estimation. The concepts of controllability, stabilizability, observability, and detectability were investigated. The property of pointwise degeneracy of linear time-invariant delay systems is considered. Necessary and sufficient conditions for three dimensional linear systems to be made pointwise degenerate by delay feedback were obtained, while sufficient conditions for this to be possible are given for higher dimensional linear systems. These results were applied to obtain solvability conditions for the minimum time output zeroing control problem by delay feedback. A representation theorem is given for conditional moment functionals of general nonlinear stochastic delay systems, and stochastic differential equations are derived for conditional moment functionals satisfying certain smoothness properties.

MMP-14 is necessary but not sufficient for invasion of three-dimensional collagen by human muscle satellite cells

PubMed Central

Lund, Dane K.; Mouly, Vincent

2014-01-01

The twenty-five known matrix metalloproteases (MMPs) and their endogenous inhibitors, tissue inhibitors of metalloproteases (TIMPs), mediate cell invasion through the extracellular matrix (ECM). In a comparative three-dimensional assay, we analyzed human and mouse satellite cells' competence to invade an artificial ECM (collagen I). We identified a single MMP that 1) is expressed by human muscle satellite cells; 2) is induced at the mRNA/protein level by adhesion to collagen I; and 3) is necessary for invasion into a collagen I matrix. Interestingly, murine satellite cells neither express this MMP, nor invade the collagen matrix. However, exogenous human MMP-14 is not sufficient to induce invasion of a collagen matrix by murine cells, emphasizing species differences. PMID:24898588

Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping

NASA Astrophysics Data System (ADS)

Bongiovanni, Marie N.; Godet, Julien; Horrocks, Mathew H.; Tosatto, Laura; Carr, Alexander R.; Wirthensohn, David C.; Ranasinghe, Rohan T.; Lee, Ji-Eun; Ponjavic, Aleks; Fritz, Joelle V.; Dobson, Christopher M.; Klenerman, David; Lee, Steven F.

2016-12-01

Super-resolution microscopy allows biological systems to be studied at the nanoscale, but has been restricted to providing only positional information. Here, we show that it is possible to perform multi-dimensional super-resolution imaging to determine both the position and the environmental properties of single-molecule fluorescent emitters. The method presented here exploits the solvatochromic and fluorogenic properties of nile red to extract both the emission spectrum and the position of each dye molecule simultaneously enabling mapping of the hydrophobicity of biological structures. We validated this by studying synthetic lipid vesicles of known composition. We then applied both to super-resolve the hydrophobicity of amyloid aggregates implicated in neurodegenerative diseases, and the hydrophobic changes in mammalian cell membranes. Our technique is easily implemented by inserting a transmission diffraction grating into the optical path of a localization-based super-resolution microscope, enabling all the information to be extracted simultaneously from a single image plane.

Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping

PubMed Central

Bongiovanni, Marie N.; Godet, Julien; Horrocks, Mathew H.; Tosatto, Laura; Carr, Alexander R.; Wirthensohn, David C.; Ranasinghe, Rohan T.; Lee, Ji-Eun; Ponjavic, Aleks; Fritz, Joelle V.; Dobson, Christopher M.; Klenerman, David; Lee, Steven F.

2016-01-01

Super-resolution microscopy allows biological systems to be studied at the nanoscale, but has been restricted to providing only positional information. Here, we show that it is possible to perform multi-dimensional super-resolution imaging to determine both the position and the environmental properties of single-molecule fluorescent emitters. The method presented here exploits the solvatochromic and fluorogenic properties of nile red to extract both the emission spectrum and the position of each dye molecule simultaneously enabling mapping of the hydrophobicity of biological structures. We validated this by studying synthetic lipid vesicles of known composition. We then applied both to super-resolve the hydrophobicity of amyloid aggregates implicated in neurodegenerative diseases, and the hydrophobic changes in mammalian cell membranes. Our technique is easily implemented by inserting a transmission diffraction grating into the optical path of a localization-based super-resolution microscope, enabling all the information to be extracted simultaneously from a single image plane. PMID:27929085

UWB Tracking System Design with TDOA Algorithm

NASA Technical Reports Server (NTRS)

Ni, Jianjun; Arndt, Dickey; Ngo, Phong; Phan, Chau; Gross, Julia; Dusl, John; Schwing, Alan

2006-01-01

This presentation discusses an ultra-wideband (UWB) tracking system design effort using a tracking algorithm TDOA (Time Difference of Arrival). UWB technology is exploited to implement the tracking system due to its properties, such as high data rate, fine time resolution, and low power spectral density. A system design using commercially available UWB products is proposed. A two-stage weighted least square method is chosen to solve the TDOA non-linear equations. Matlab simulations in both two-dimensional space and three-dimensional space show that the tracking algorithm can achieve fine tracking resolution with low noise TDOA data. The error analysis reveals various ways to improve the tracking resolution. Lab experiments demonstrate the UWBTDOA tracking capability with fine resolution. This research effort is motivated by a prototype development project Mini-AERCam (Autonomous Extra-vehicular Robotic Camera), a free-flying video camera system under development at NASA Johnson Space Center for aid in surveillance around the International Space Station (ISS).

Computational methods for constructing protein structure models from 3D electron microscopy maps.

PubMed

Esquivel-Rodríguez, Juan; Kihara, Daisuke

2013-10-01

Protein structure determination by cryo-electron microscopy (EM) has made significant progress in the past decades. Resolutions of EM maps have been improving as evidenced by recently reported structures that are solved at high resolutions close to 3Å. Computational methods play a key role in interpreting EM data. Among many computational procedures applied to an EM map to obtain protein structure information, in this article we focus on reviewing computational methods that model protein three-dimensional (3D) structures from a 3D EM density map that is constructed from two-dimensional (2D) maps. The computational methods we discuss range from de novo methods, which identify structural elements in an EM map, to structure fitting methods, where known high resolution structures are fit into a low-resolution EM map. A list of available computational tools is also provided. Copyright © 2013 Elsevier Inc. All rights reserved.

High resolution multidetector CT aided tissue analysis and quantification of lung fibrosis

NASA Astrophysics Data System (ADS)

Zavaletta, Vanessa A.; Karwoski, Ronald A.; Bartholmai, Brian; Robb, Richard A.

2006-03-01

Idiopathic pulmonary fibrosis (IPF, also known as Idiopathic Usual Interstitial Pneumontis, pathologically) is a progressive diffuse lung disease which has a median survival rate of less than four years with a prevalence of 15-20/100,000 in the United States. Global function changes are measured by pulmonary function tests and the diagnosis and extent of pulmonary structural changes are typically assessed by acquiring two-dimensional high resolution CT (HRCT) images. The acquisition and analysis of volumetric high resolution Multi-Detector CT (MDCT) images with nearly isotropic pixels offers the potential to measure both lung function and structure. This paper presents a new approach to three dimensional lung image analysis and classification of normal and abnormal structures in lungs with IPF.

Far-field photostable optical nanoscopy (PHOTON) for real-time super-resolution single-molecular imaging of signaling pathways of single live cells

NASA Astrophysics Data System (ADS)

Huang, Tao; Browning, Lauren M.; Xu, Xiao-Hong Nancy

2012-04-01

Cellular signaling pathways play crucial roles in cellular functions and design of effective therapies. Unfortunately, study of cellular signaling pathways remains formidably challenging because sophisticated cascades are involved, and a few molecules are sufficient to trigger signaling responses of a single cell. Here we report the development of far-field photostable-optical-nanoscopy (PHOTON) with photostable single-molecule-nanoparticle-optical-biosensors (SMNOBS) for mapping dynamic cascades of apoptotic signaling pathways of single live cells in real-time at single-molecule (SM) and nanometer (nm) resolutions. We have quantitatively imaged single ligand molecules (tumor necrosis factor α, TNFα) and their binding kinetics with their receptors (TNFR1) on single live cells; tracked formation and internalization of their clusters and their initiation of intracellular signaling pathways in real-time; and studied apoptotic signaling dynamics and mechanisms of single live cells with sufficient temporal and spatial resolutions. This study provides new insights into complex real-time dynamic cascades and molecular mechanisms of apoptotic signaling pathways of single live cells. PHOTON provides superior imaging and sensing capabilities and SMNOBS offer unrivaled biocompatibility and photostability, which enable probing of signaling pathways of single live cells in real-time at SM and nm resolutions.Cellular signaling pathways play crucial roles in cellular functions and design of effective therapies. Unfortunately, study of cellular signaling pathways remains formidably challenging because sophisticated cascades are involved, and a few molecules are sufficient to trigger signaling responses of a single cell. Here we report the development of far-field photostable-optical-nanoscopy (PHOTON) with photostable single-molecule-nanoparticle-optical-biosensors (SMNOBS) for mapping dynamic cascades of apoptotic signaling pathways of single live cells in real-time at single-molecule (SM) and nanometer (nm) resolutions. We have quantitatively imaged single ligand molecules (tumor necrosis factor α, TNFα) and their binding kinetics with their receptors (TNFR1) on single live cells; tracked formation and internalization of their clusters and their initiation of intracellular signaling pathways in real-time; and studied apoptotic signaling dynamics and mechanisms of single live cells with sufficient temporal and spatial resolutions. This study provides new insights into complex real-time dynamic cascades and molecular mechanisms of apoptotic signaling pathways of single live cells. PHOTON provides superior imaging and sensing capabilities and SMNOBS offer unrivaled biocompatibility and photostability, which enable probing of signaling pathways of single live cells in real-time at SM and nm resolutions. Electronic supplementary information (ESI) available. See DOI: 10.1039/c2nr11739h

Combustion and Magnetohydrodynamic Processes in Advanced Pulse Detonation Rocket Engines

NASA Astrophysics Data System (ADS)

Cole, Lord Kahil

A number of promising alternative rocket propulsion concepts have been developed over the past two decades that take advantage of unsteady combustion waves in order to produce thrust. These concepts include the Pulse Detonation Rocket Engine (PDRE), in which repetitive ignition, propagation, and reflection of detonations and shocks can create a high pressure chamber from which gases may be exhausted in a controlled manner. The Pulse Detonation Rocket Induced Magnetohydrodynamic Ejector (PDRIME) is a modification of the basic PDRE concept, developed by Cambier (1998), which has the potential for performance improvements based on magnetohydrodynamic (MHD) thrust augmentation. The PDRIME has the advantage of both low combustion chamber seeding pressure, per the PDRE concept, and efficient energy distribution in the system, per the rocket-induced MHD ejector (RIME) concept of Cole, et al. (1995). In the initial part of this thesis, we explore flow and performance characteristics of different configurations of the PDRIME, assuming quasi-one-dimensional transient flow and global representations of the effects of MHD phenomena on the gas dynamics. By utilizing high-order accurate solvers, we thus are able to investigate the fundamental physical processes associated with the PDRIME and PDRE concepts and identify potentially promising operating regimes. In the second part of this investigation, the detailed coupling of detonations and electric and magnetic fields are explored. First, a one-dimensional spark-ignited detonation with complex reaction kinetics is fully evaluated and the mechanisms for the different instabilities are analyzed. It is found that complex kinetics in addition to sufficient spatial resolution are required to be able to quantify high frequency as well as low frequency detonation instability modes. Armed with this quantitative understanding, we then examine the interaction of a propagating detonation and the applied MHD, both in one-dimensional and two-dimensional transient simulations. The dynamics of the detonation are found to be affected by the application of magnetic and electric fields. We find that the regularity of one-dimensional cesium-seeded detonations can be significantly altered by reasonable applied magnetic fields (Bz ≤ 8T), but that it takes a stronger applied field (Bz > 16T) to significantly alter the cellular structure and detonation velocity of a two-dimensional detonation in the time in which these phenomena were observed. This observation is likely attributed to the additional coupling of the two-dimensional detonation with the transverse waves, which are not captured in the one-dimensional simulations. Future studies involving full ionization kinetics including collisional-radiative processes, will be used to examine these processes in further detail.

Optimization of the first dimension for separation by two-dimensional gel electrophoresis of basic proteins from human brain tissue.

PubMed

Pennington, Kyla; McGregor, Emma; Beasley, Clare L; Everall, Ian; Cotter, David; Dunn, Michael J

2004-01-01

A major cause of poor resolution in the alkaline pH range of two-dimensional electrophoresis (2-DE) gels is unsatisfactory separation of basic proteins in the first dimension. We have compared methods for the separation of basic proteins in the isoelectric focusing dimension of human brain proteins. The combined use of anodic cup-loading and the hydroxyethyldisulphide containing solution (DeStreak) produced better resolution in both analytical and micropreparative protein loaded 2-DE gels than the other methods investigated.

Multiresolution MR elastography using nonlinear inversion

PubMed Central

McGarry, M. D. J.; Van Houten, E. E. W.; Johnson, C. L.; Georgiadis, J. G.; Sutton, B. P.; Weaver, J. B.; Paulsen, K. D.

2012-01-01

Purpose: Nonlinear inversion (NLI) in MR elastography requires discretization of the displacement field for a finite element (FE) solution of the “forward problem”, and discretization of the unknown mechanical property field for the iterative solution of the “inverse problem”. The resolution requirements for these two discretizations are different: the forward problem requires sufficient resolution of the displacement FE mesh to ensure convergence, whereas lowering the mechanical property resolution in the inverse problem stabilizes the mechanical property estimates in the presence of measurement noise. Previous NLI implementations use the same FE mesh to support the displacement and property fields, requiring a trade-off between the competing resolution requirements. Methods: This work implements and evaluates multiresolution FE meshes for NLI elastography, allowing independent discretizations of the displacements and each mechanical property parameter to be estimated. The displacement resolution can then be selected to ensure mesh convergence, and the resolution of the property meshes can be independently manipulated to control the stability of the inversion. Results: Phantom experiments indicate that eight nodes per wavelength (NPW) are sufficient for accurate mechanical property recovery, whereas mechanical property estimation from 50 Hz in vivo brain data stabilizes once the displacement resolution reaches 1.7 mm (approximately 19 NPW). Viscoelastic mechanical property estimates of in vivo brain tissue show that subsampling the loss modulus while holding the storage modulus resolution constant does not substantially alter the storage modulus images. Controlling the ratio of the number of measurements to unknown mechanical properties by subsampling the mechanical property distributions (relative to the data resolution) improves the repeatability of the property estimates, at a cost of modestly decreased spatial resolution. Conclusions: Multiresolution NLI elastography provides a more flexible framework for mechanical property estimation compared to previous single mesh implementations. PMID:23039674

Improved imaging of cochlear nerve hypoplasia using a 3-Tesla variable flip-angle turbo spin-echo sequence and a 7-cm surface coil.

PubMed

Giesemann, Anja M; Raab, Peter; Lyutenski, Stefan; Dettmer, Sabine; Bültmann, Eva; Frömke, Cornelia; Lenarz, Thomas; Lanfermann, Heinrich; Goetz, Friedrich

2014-03-01

Magnetic resonance imaging of the temporal bone has an important role in decision making with regard to cochlea implantation, especially in children with cochlear nerve deficiency. The purpose of this study was to evaluate the usefulness of the combination of an advanced high-resolution T2-weighted sequence with a surface coil in a 3-Tesla magnetic resonance imaging scanner in cases of suspected cochlear nerve aplasia. Prospective study. Seven patients with cochlear nerve hypoplasia or aplasia were prospectively examined using a high-resolution three-dimensional variable flip-angle turbo spin-echo sequence using a surface coil, and the images were compared with the same sequence in standard resolution using a standard head coil. Three neuroradiologists evaluated the magnetic resonance images independently, rating the visibility of the nerves in diagnosing hypoplasia or aplasia. Eight ears in seven patients with hypoplasia or aplasia of the cochlear nerve were examined. The average age was 2.7 years (range, 9 months-5 years). Seven ears had accompanying malformations. The inter-rater reliability in diagnosing hypoplasia or aplasia was greater using the high-resolution three-dimensional variable flip-angle turbo spin-echo sequence (fixed-marginal kappa: 0.64) than with the same sequence in lower resolution (fixed-marginal kappa: 0.06). Examining cases of suspected cochlear nerve aplasia using the high-resolution three-dimensional variable flip-angle turbo spin-echo sequence in combination with a surface coil shows significant improvement over standard methods. © 2013 The American Laryngological, Rhinological and Otological Society, Inc.

Utilization of high resolution computed tomography to visualize the three dimensional structure and function of plant vasculature

USDA-ARS?s Scientific Manuscript database

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being used to evaluate the structure and function of plant xylem network in three dimensions (3D). HRCT imaging is based on the same principles as medi...

FASTPM: a new scheme for fast simulations of dark matter and haloes

NASA Astrophysics Data System (ADS)

Feng, Yu; Chu, Man-Yat; Seljak, Uroš; McDonald, Patrick

2016-12-01

We introduce FASTPM, a highly scalable approximated particle mesh (PM) N-body solver, which implements the PM scheme enforcing correct linear displacement (1LPT) evolution via modified kick and drift factors. Employing a two-dimensional domain decomposing scheme, FASTPM scales extremely well with a very large number of CPUs. In contrast to Comoving-Lagrangian (COLA) approach, we do not require to split the force or track separately the 2LPT solution, reducing the code complexity and memory requirements. We compare FASTPM with different number of steps (Ns) and force resolution factor (B) against three benchmarks: halo mass function from friends-of-friends halo finder; halo and dark matter power spectrum; and cross-correlation coefficient (or stochasticity), relative to a high-resolution TREEPM simulation. We show that the modified time stepping scheme reduces the halo stochasticity when compared to COLA with the same number of steps and force resolution. While increasing Ns and B improves the transfer function and cross-correlation coefficient, for many applications FASTPM achieves sufficient accuracy at low Ns and B. For example, Ns = 10 and B = 2 simulation provides a substantial saving (a factor of 10) of computing time relative to Ns = 40, B = 3 simulation, yet the halo benchmarks are very similar at z = 0. We find that for abundance matched haloes the stochasticity remains low even for Ns = 5. FASTPM compares well against less expensive schemes, being only 7 (4) times more expensive than 2LPT initial condition generator for Ns = 10 (Ns = 5). Some of the applications where FASTPM can be useful are generating a large number of mocks, producing non-linear statistics where one varies a large number of nuisance or cosmological parameters, or serving as part of an initial conditions solver.

Evaluating galactic habitability using high-resolution cosmological simulations of galaxy formation

NASA Astrophysics Data System (ADS)

Forgan, Duncan; Dayal, Pratika; Cockell, Charles; Libeskind, Noam

2017-01-01

We present the first model that couples high-resolution simulations of the formation of local group galaxies with calculations of the galactic habitable zone (GHZ), a region of space which has sufficient metallicity to form terrestrial planets without being subject to hazardous radiation. These simulations allow us to make substantial progress in mapping out the asymmetric three-dimensional GHZ and its time evolution for the Milky Way (MW) and Triangulum (M33) galaxies, as opposed to works that generally assume an azimuthally symmetric GHZ. Applying typical habitability metrics to MW and M33, we find that while a large number of habitable planets exist as close as a few kiloparsecs from the galactic centre, the probability of individual planetary systems being habitable rises as one approaches the edge of the stellar disc. Tidal streams and satellite galaxies also appear to be fertile grounds for habitable planet formation. In short, we find that both galaxies arrive at similar GHZs by different evolutionary paths, as measured by the first and third quartiles of surviving biospheres. For the MW, this interquartile range begins as a narrow band at large radii, expanding to encompass much of the Galaxy at intermediate times before settling at a range of 2-13 kpc. In the case of M33, the opposite behaviour occurs - the initial and final interquartile ranges are quite similar, showing gradual evolution. This suggests that Galaxy assembly history strongly influences the time evolution of the GHZ, which will affect the relative time lag between biospheres in different galactic locations. We end by noting the caveats involved in such studies and demonstrate that high-resolution cosmological simulations will play a vital role in understanding habitability on galactic scales, provided that these simulations accurately resolve chemical evolution.

High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf

PubMed Central

Weis, Jan; Bruvold, Morten; Ortiz-Nieto, Francisco; Ahlström, Håkan

2014-01-01

Background This study exploits the speed benefits of echo-planar spectroscopic imaging (EPSI) to acquire lipid spectra of skeletal muscle. The main purpose was to develop a high-resolution EPSI technique for clinical MR scanner, to visualise the bulk magnetic susceptibility (BMS) shifts of extra-myocellular lipid (EMCL) spectral lines, and to investigate the feasibility of this method for the assessment of intra-myocellular (IMCL) lipids. Methods The study group consisted of six healthy volunteers. A two dimensional EPSI sequence with point-resolved spectroscopy (PRESS) spatial localization was implemented on a 3T clinical MR scanner. Measurements were performed by means of 64×64 spatial matrix and nominal voxel size 3×3×15 mm3. The total net measurement time was 3 min 12 sec for non-water-suppressed (1 acquisition) and 12 min 48 sec for water-suppressed scans (4 acquisitions). Results Spectra of the human calf had a very good signal-to-noise ratio and linewidths sufficient to differentiate IMCL resonances from EMCL. The use of a large spatial matrix reduces inter-voxel signal contamination of the strong EMCL signals. Small voxels enabled visualisation of the methylene EMCL spectral line splitting and their BMS shifts up to 0.5 ppm relative to the correspondent IMCL line. The mean soleus muscle IMCL content of our six volunteers was 0.30±0.10 vol% (range 0.18–0.46) or 3.6±1.2 mmol/kg wet weight (range: 2.1–5.4). Conclusion This study demonstrates that high-spatial resolution PRESS EPSI of the muscle lipids is feasible on standard clinical scanners. PMID:24498129

Dynamic PCr and pH imaging of human calf muscles during exercise and recovery using (31) P gradient-Echo MRI at 7 Tesla.

PubMed

Schmid, Albrecht Ingo; Meyerspeer, Martin; Robinson, Simon Daniel; Goluch, Sigrun; Wolzt, Michael; Fiedler, Georg Bernd; Bogner, Wolfgang; Laistler, Elmar; Krššák, Martin; Moser, Ewald; Trattnig, Siegfried; Valkovič, Ladislav

2016-06-01

Simultaneous acquisition of spatially resolved (31) P-MRI data for evaluation of muscle specific energy metabolism, i.e., PCr and pH kinetics. A three-dimensional (3D) gradient-echo sequence for multiple frequency-selective excitations of the PCr and Pi signals in an interleaved sampling scheme was developed and tested at 7 Tesla (T). The pH values were derived from the chemical shift-induced phase difference between the resonances. The achieved spatial resolution was ∼2 mL with image acquisition time below 6 s. Ten healthy volunteers were studied performing plantar flexions during the delay between (31) P-MRI acquisitions, yielding a temporal resolution of 9-10 s. Signal from anatomically matched regions of interest had sufficient signal-to-noise ratio to allow single-acquisition PCr and pH quantification. The Pi signal was clearly detected in voxels of actively exercising muscles. The PCr depletions were in gastrocnemius 42 ± 14% (medialis), 48 ± 17% (lateralis) and in soleus 20 ± 11%. The end exercise pH values were 6.74 ± 0.18 and 6.65 ± 0.27 for gastrocnemius medialis and lateralis, respectively, and 6.96 ± 0.12 for soleus muscle. Simultaneous acquisition of PCr and Pi images with high temporal resolution, suitable for measuring PCr and pH kinetics in exercise-recovery experiments, was demonstrated at 7T. This study presents a fast alternative to MRS for quantifying energy metabolism of posterior muscle groups of the lower leg. Magn Reson Med 75:2324-2331, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.

Passive Standoff Super Resolution Imaging using Spatial-Spectral Multiplexing

DTIC Science & Technology

2017-08-14

94 5.0 Four -Dimensional Object-Space Data Reconstruction Using Spatial...103 5.3 Four -dimensional scene reconstruction using SSM...transitioning to systems based on spectrally resolved longitudinal spatial coherence interferometry. This document also includes research related to four

DOE Office of Scientific and Technical Information (OSTI.GOV)

Levegruen, Sabine, E-mail: sabine.levegruen@uni-due.de; Poettgen, Christoph; Abu Jawad, Jehad

Purpose: To evaluate megavoltage computed tomography (MVCT)-based image guidance with helical tomotherapy in patients with vertebral tumors by analyzing factors influencing interobserver variability, considered as quality criterion of image guidance. Methods and Materials: Five radiation oncologists retrospectively registered 103 MVCTs in 10 patients to planning kilovoltage CTs by rigid transformations in 4 df. Interobserver variabilities were quantified using the standard deviations (SDs) of the distributions of the correction vector components about the observers' fraction mean. To assess intraobserver variabilities, registrations were repeated after {>=}4 weeks. Residual deviations after setup correction due to uncorrectable rotational errors and elastic deformations were determinedmore » at 3 craniocaudal target positions. To differentiate observer-related variations in minimizing these residual deviations across the 3-dimensional MVCT from image resolution effects, 2-dimensional registrations were performed in 30 single transverse and sagittal MVCT slices. Axial and longitudinal MVCT image resolutions were quantified. For comparison, image resolution of kilovoltage cone-beam CTs (CBCTs) and interobserver variability in registrations of 43 CBCTs were determined. Results: Axial MVCT image resolution is 3.9 lp/cm. Longitudinal MVCT resolution amounts to 6.3 mm, assessed as full-width at half-maximum of thin objects in MVCTs with finest pitch. Longitudinal CBCT resolution is better (full-width at half-maximum, 2.5 mm for CBCTs with 1-mm slices). In MVCT registrations, interobserver variability in the craniocaudal direction (SD 1.23 mm) is significantly larger than in the lateral and ventrodorsal directions (SD 0.84 and 0.91 mm, respectively) and significantly larger compared with CBCT alignments (SD 1.04 mm). Intraobserver variabilities are significantly smaller than corresponding interobserver variabilities (variance ratio [VR] 1.8-3.1). Compared with 3-dimensional registrations, 2-dimensional registrations have significantly smaller interobserver variability in the lateral and ventrodorsal directions (VR 3.8 and 2.8, respectively) but not in the craniocaudal direction (VR 0.75). Conclusion: Tomotherapy image guidance precision is affected by image resolution and residual deviations after setup correction. Eliminating the effect of residual deviations yields small interobserver variabilities with submillimeter precision in the axial plane. In contrast, interobserver variability in the craniocaudal direction is dominated by the poorer longitudinal MVCT image resolution. Residual deviations after image guidance exist and need to be considered when dose gradients ultimately achievable with image guided radiation therapy techniques are analyzed.« less

Megavoltage computed tomography image guidance with helical tomotherapy in patients with vertebral tumors: analysis of factors influencing interobserver variability.

PubMed

Levegrün, Sabine; Pöttgen, Christoph; Jawad, Jehad Abu; Berkovic, Katharina; Hepp, Rodrigo; Stuschke, Martin

2013-02-01

To evaluate megavoltage computed tomography (MVCT)-based image guidance with helical tomotherapy in patients with vertebral tumors by analyzing factors influencing interobserver variability, considered as quality criterion of image guidance. Five radiation oncologists retrospectively registered 103 MVCTs in 10 patients to planning kilovoltage CTs by rigid transformations in 4 df. Interobserver variabilities were quantified using the standard deviations (SDs) of the distributions of the correction vector components about the observers' fraction mean. To assess intraobserver variabilities, registrations were repeated after ≥4 weeks. Residual deviations after setup correction due to uncorrectable rotational errors and elastic deformations were determined at 3 craniocaudal target positions. To differentiate observer-related variations in minimizing these residual deviations across the 3-dimensional MVCT from image resolution effects, 2-dimensional registrations were performed in 30 single transverse and sagittal MVCT slices. Axial and longitudinal MVCT image resolutions were quantified. For comparison, image resolution of kilovoltage cone-beam CTs (CBCTs) and interobserver variability in registrations of 43 CBCTs were determined. Axial MVCT image resolution is 3.9 lp/cm. Longitudinal MVCT resolution amounts to 6.3 mm, assessed as full-width at half-maximum of thin objects in MVCTs with finest pitch. Longitudinal CBCT resolution is better (full-width at half-maximum, 2.5 mm for CBCTs with 1-mm slices). In MVCT registrations, interobserver variability in the craniocaudal direction (SD 1.23 mm) is significantly larger than in the lateral and ventrodorsal directions (SD 0.84 and 0.91 mm, respectively) and significantly larger compared with CBCT alignments (SD 1.04 mm). Intraobserver variabilities are significantly smaller than corresponding interobserver variabilities (variance ratio [VR] 1.8-3.1). Compared with 3-dimensional registrations, 2-dimensional registrations have significantly smaller interobserver variability in the lateral and ventrodorsal directions (VR 3.8 and 2.8, respectively) but not in the craniocaudal direction (VR 0.75). Tomotherapy image guidance precision is affected by image resolution and residual deviations after setup correction. Eliminating the effect of residual deviations yields small interobserver variabilities with submillimeter precision in the axial plane. In contrast, interobserver variability in the craniocaudal direction is dominated by the poorer longitudinal MVCT image resolution. Residual deviations after image guidance exist and need to be considered when dose gradients ultimately achievable with image guided radiation therapy techniques are analyzed. Copyright © 2013 Elsevier Inc. All rights reserved.

Synthesis and identification of three-dimensional faces from image(s) and three-dimensional generic models

NASA Astrophysics Data System (ADS)

Liu, Zexi; Cohen, Fernand

2017-11-01

We describe an approach for synthesizing a three-dimensional (3-D) face structure from an image or images of a human face taken at a priori unknown poses using gender and ethnicity specific 3-D generic models. The synthesis process starts with a generic model, which is personalized as images of the person become available using preselected landmark points that are tessellated to form a high-resolution triangular mesh. From a single image, two of the three coordinates of the model are reconstructed in accordance with the given image of the person, while the third coordinate is sampled from the generic model, and the appearance is made in accordance with the image. With multiple images, all coordinates and appearance are reconstructed in accordance with the observed images. This method allows for accurate pose estimation as well as face identification in 3-D rendering of a difficult two-dimensional (2-D) face recognition problem into a much simpler 3-D surface matching problem. The estimation of the unknown pose is achieved using the Levenberg-Marquardt optimization process. Encouraging experimental results are obtained in a controlled environment with high-resolution images under a good illumination condition, as well as for images taken in an uncontrolled environment under arbitrary illumination with low-resolution cameras.

A call for government accountability to achieve national self-sufficiency in organ donation and transplantation.

PubMed

Delmonico, Francis L; Domínguez-Gil, Beatriz; Matesanz, Rafael; Noel, Luc

2011-10-15

Roughly 100,000 patients worldwide undergo organ transplantation annually, but many other patients remain on waiting lists. Transplantation rates vary substantially across countries. Affluent patients in nations with long waiting lists do not always wait for donations from within their own countries. Commercially driven transplantation, however, does not always ensure proper medical care of recipients or donors, and might lengthen waiting times for resident patients or increase the illegal and unethical purchase of organs from living donors. Governments should systematically address the needs of their countries according to a legal framework. Medical strategies to prevent end-stage organ failure must also be implemented. In view of the Madrid Resolution, the Declaration of Istanbul, and the 63rd World Health Assembly Resolution, a new paradigm of national self-sufficiency is needed. Each country or region should strive to provide a sufficient number of organs from within its own population, guided by WHO ethics principles. Copyright © 2011 Elsevier Ltd. All rights reserved.

Using Harry Potter to Bridge Higher Dimensionality in Mathematics and High-interest Literature

ERIC Educational Resources Information Center

Boerman-Cornell, William; Klanderman, David; Schut, Alexa

2017-01-01

The Harry Potter series is a favorite for out-of-school reading and has been used in school, largely as an object of study in language arts. Using a content analysis to highlight the ways in which J.K. Rowling's work could be used to teach higher dimensionality in math, the authors argues that the content is sufficient in such books to engage the…

Fokker-Planck simulation of runaway electron generation in disruptions with the hot-tail effect

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nuga, H., E-mail: nuga@p-grp.nucleng.kyoto-u.ac.jp; Fukuyama, A.; Yagi, M.

2016-06-15

To study runaway electron generation in disruptions, we have extended the three-dimensional (two-dimensional in momentum space; one-dimensional in the radial direction) Fokker-Planck code, which describes the evolution of the relativistic momentum distribution function of electrons and the induced toroidal electric field in a self-consistent manner. A particular focus is placed on the hot-tail effect in two-dimensional momentum space. The effect appears if the drop of the background plasma temperature is sufficiently rapid compared with the electron-electron slowing down time for a few times of the pre-quench thermal velocity. It contributes to not only the enhancement of the primary runaway electronmore » generation but also the broadening of the runaway electron distribution in the pitch angle direction. If the thermal energy loss during the major disruption is assumed to be isotropic, there are hot-tail electrons that have sufficiently large perpendicular momentum, and the runaway electron distribution becomes broader in the pitch angle direction. In addition, the pitch angle scattering also yields the broadening. Since the electric field is reduced due to the burst of runaway electron generation, the time required for accelerating electrons to the runaway region becomes longer. The longer acceleration period makes the pitch-angle scattering more effective.« less

Temporal focusing microscopy combined with three-dimensional structured illumination

NASA Astrophysics Data System (ADS)

Isobe, Keisuke; Toda, Keisuke; Song, Qiyuan; Kannari, Fumihiko; Kawano, Hiroyuki; Miyawaki, Atsushi; Midorikawa, Katsumi

2017-05-01

Temporal focusing microscopy provides the optical sectioning capability in wide-field two-photon fluorescence imaging. Here, we demonstrate temporal focusing microscopy combined with three-dimensional structured illumination, which enables us to enhance the three-dimensional spatial resolution and reject the background fluorescence. Experimentally, the periodic pattern of the illumination was produced not only in the lateral direction but also in the axial direction by the interference between three temporal focusing pulses, which were easily generated using a digital micromirror device. The lateral resolution and optical sectioning capability were successfully enhanced by factors of 1.6 and 3.6, respectively, compared with those of temporal focusing microscopy. In the two-photon fluorescence imaging of a tissue-like phantom, the out-of-focus background fluorescence and the scattered background fluorescence could also be rejected.

Three-dimensional computational aerodynamics in the 1980's

NASA Technical Reports Server (NTRS)

Lomax, H.

1978-01-01

The future requirements for constructing codes that can be used to compute three-dimensional flows about aerodynamic shapes should be assessed in light of the constraints imposed by future computer architectures and the reality of usable algorithms that can provide practical three-dimensional simulations. On the hardware side, vector processing is inevitable in order to meet the CPU speeds required. To cope with three-dimensional geometries, massive data bases with fetch/store conflicts and transposition problems are inevitable. On the software side, codes must be prepared that: (1) can be adapted to complex geometries, (2) can (at the very least) predict the location of laminar and turbulent boundary layer separation, and (3) will converge rapidly to sufficiently accurate solutions.

Two Dimensional Mechanism for Insect Hovering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jane Wang, Z.

2000-09-04

Resolved computation of two dimensional insect hovering shows for the first time that a two dimensional hovering motion can generate enough lift to support a typical insect weight. The computation reveals a two dimensional mechanism of creating a downward dipole jet of counterrotating vortices, which are formed from leading and trailing edge vortices. The vortex dynamics further elucidates the role of the phase relation between the wing translation and rotation in lift generation and explains why the instantaneous forces can reach a periodic state after only a few strokes. The model predicts the lower limits in Reynolds number and amplitudemore » above which the averaged forces are sufficient. (c) 2000 The American Physical Society.« less

Enhanced spectral resolution by high-dimensional NMR using the filter diagonalization method and “hidden” dimensions

PubMed Central

Meng, Xi; Nguyen, Bao D.; Ridge, Clark; Shaka, A. J.

2009-01-01

High-dimensional (HD) NMR spectra have poorer digital resolution than low-dimensional (LD) spectra, for a fixed amount of experiment time. This has led to “reduced-dimensionality” strategies, in which several LD projections of the HD NMR spectrum are acquired, each with higher digital resolution; an approximate HD spectrum is then inferred by some means. We propose a strategy that moves in the opposite direction, by adding more time dimensions to increase the information content of the data set, even if only a very sparse time grid is used in each dimension. The full HD time-domain data can be analyzed by the Filter Diagonalization Method (FDM), yielding very narrow resonances along all of the frequency axes, even those with sparse sampling. Integrating over the added dimensions of HD FDM NMR spectra reconstitutes LD spectra with enhanced resolution, often more quickly than direct acquisition of the LD spectrum with a larger number of grid points in each of the fewer dimensions. If the extra dimensions do not appear in the final spectrum, and are used solely to boost information content, we propose the moniker hidden-dimension NMR. This work shows that HD peaks have unmistakable frequency signatures that can be detected as single HD objects by an appropriate algorithm, even though their patterns would be tricky for a human operator to visualize or recognize, and even if digital resolution in an HD FT spectrum is very coarse compared with natural line widths. PMID:18926747

High-resolution non-destructive three-dimensional imaging of integrated circuits

NASA Astrophysics Data System (ADS)

Holler, Mirko; Guizar-Sicairos, Manuel; Tsai, Esther H. R.; Dinapoli, Roberto; Müller, Elisabeth; Bunk, Oliver; Raabe, Jörg; Aeppli, Gabriel

2017-03-01

Modern nanoelectronics has advanced to a point at which it is impossible to image entire devices and their interconnections non-destructively because of their small feature sizes and the complex three-dimensional structures resulting from their integration on a chip. This metrology gap implies a lack of direct feedback between design and manufacturing processes, and hampers quality control during production, shipment and use. Here we demonstrate that X-ray ptychography—a high-resolution coherent diffractive imaging technique—can create three-dimensional images of integrated circuits of known and unknown designs with a lateral resolution in all directions down to 14.6 nanometres. We obtained detailed device geometries and corresponding elemental maps, and show how the devices are integrated with each other to form the chip. Our experiments represent a major advance in chip inspection and reverse engineering over the traditional destructive electron microscopy and ion milling techniques. Foreseeable developments in X-ray sources, optics and detectors, as well as adoption of an instrument geometry optimized for planar rather than cylindrical samples, could lead to a thousand-fold increase in efficiency, with concomitant reductions in scan times and voxel sizes.

Analysis of human plasma lipids by using comprehensive two-dimensional gas chromatography with dual detection and with the support of high-resolution time-of-flight mass spectrometry for structural elucidation.

PubMed

Salivo, Simona; Beccaria, Marco; Sullini, Giuseppe; Tranchida, Peter Q; Dugo, Paola; Mondello, Luigi

2015-01-01

The main focus of the present research is the analysis of the unsaponifiable lipid fraction of human plasma by using data derived from comprehensive two-dimensional gas chromatography with dual quadrupole mass spectrometry and flame ionization detection. This approach enabled us to attain both mass spectral information and analyte percentage data. Furthermore, gas chromatography coupled with high-resolution time-of-flight mass spectrometry was used to increase the reliability of identification of several unsaponifiable lipid constituents. The synergism between both the high-resolution gas chromatography and mass spectrometry processes enabled us to attain a more in-depth knowledge of the unsaponifiable fraction of human plasma. Additionally, information was attained on the fatty acid and triacylglycerol composition of the plasma samples, subjected to investigation by using comprehensive two-dimensional gas chromatography with dual quadrupole mass spectrometry and flame ionization detection and high-performance liquid chromatography with atmospheric pressure chemical ionization quadrupole mass spectrometry, respectively. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

High-resolution non-destructive three-dimensional imaging of integrated circuits.

PubMed

Holler, Mirko; Guizar-Sicairos, Manuel; Tsai, Esther H R; Dinapoli, Roberto; Müller, Elisabeth; Bunk, Oliver; Raabe, Jörg; Aeppli, Gabriel

2017-03-15

Modern nanoelectronics has advanced to a point at which it is impossible to image entire devices and their interconnections non-destructively because of their small feature sizes and the complex three-dimensional structures resulting from their integration on a chip. This metrology gap implies a lack of direct feedback between design and manufacturing processes, and hampers quality control during production, shipment and use. Here we demonstrate that X-ray ptychography-a high-resolution coherent diffractive imaging technique-can create three-dimensional images of integrated circuits of known and unknown designs with a lateral resolution in all directions down to 14.6 nanometres. We obtained detailed device geometries and corresponding elemental maps, and show how the devices are integrated with each other to form the chip. Our experiments represent a major advance in chip inspection and reverse engineering over the traditional destructive electron microscopy and ion milling techniques. Foreseeable developments in X-ray sources, optics and detectors, as well as adoption of an instrument geometry optimized for planar rather than cylindrical samples, could lead to a thousand-fold increase in efficiency, with concomitant reductions in scan times and voxel sizes.

Metallic powder-bed based 3D printing of cellular scaffolds for orthopaedic implants: A state-of-the-art review on manufacturing, topological design, mechanical properties and biocompatibility.

PubMed

Tan, X P; Tan, Y J; Chow, C S L; Tor, S B; Yeong, W Y

2017-07-01

Metallic cellular scaffold is one of the best choices for orthopaedic implants as a replacement of human body parts, which could improve life quality and increase longevity for the people needed. Unlike conventional methods of making cellular scaffolds, three-dimensional (3D) printing or additive manufacturing opens up new possibilities to fabricate those customisable intricate designs with highly interconnected pores. In the past decade, metallic powder-bed based 3D printing methods emerged and the techniques are becoming increasingly mature recently, where selective laser melting (SLM) and selective electron beam melting (SEBM) are the two representatives. Due to the advantages of good dimensional accuracy, high build resolution, clean build environment, saving materials, high customisability, etc., SLM and SEBM show huge potential in direct customisable manufacturing of metallic cellular scaffolds for orthopaedic implants. Ti-6Al-4V to date is still considered to be the optimal materials for producing orthopaedic implants due to its best combination of biocompatibility, corrosion resistance and mechanical properties. This paper presents a state-of-the-art overview mainly on manufacturing, topological design, mechanical properties and biocompatibility of cellular Ti-6Al-4V scaffolds via SLM and SEBM methods. Current manufacturing limitations, topological shortcomings, uncertainty of biocompatible test were sufficiently discussed herein. Future perspectives and recommendations were given at the end. Copyright © 2017 Elsevier B.V. All rights reserved.

Conjugate Compressible Fluid Flow and Heat Transfer in Ducts

NASA Technical Reports Server (NTRS)

Cross, M. F.

2011-01-01

A computational approach to modeling transient, compressible fluid flow with heat transfer in long, narrow ducts is presented. The primary application of the model is for analyzing fluid flow and heat transfer in solid propellant rocket motor nozzle joints during motor start-up, but the approach is relevant to a wide range of analyses involving rapid pressurization and filling of ducts. Fluid flow is modeled through solution of the spatially one-dimensional, transient Euler equations. Source terms are included in the governing equations to account for the effects of wall friction and heat transfer. The equation solver is fully-implicit, thus providing greater flexibility than an explicit solver. This approach allows for resolution of pressure wave effects on the flow as well as for fast calculation of the steady-state solution when a quasi-steady approach is sufficient. Solution of the one-dimensional Euler equations with source terms significantly reduces computational run times compared to general purpose computational fluid dynamics packages solving the Navier-Stokes equations with resolved boundary layers. In addition, conjugate heat transfer is more readily implemented using the approach described in this paper than with most general purpose computational fluid dynamics packages. The compressible flow code has been integrated with a transient heat transfer solver to analyze heat transfer between the fluid and surrounding structure. Conjugate fluid flow and heat transfer solutions are presented. The author is unaware of any previous work available in the open literature which uses the same approach described in this paper.

Gamma-Ray Imager With High Spatial And Spectral Resolution

NASA Technical Reports Server (NTRS)

Callas, John L.; Varnell, Larry S.; Wheaton, William A.; Mahoney, William A.

1996-01-01

Gamma-ray instrument developed to enable both two-dimensional imaging at relatively high spatial resolution and spectroscopy at fractional-photon-energy resolution of about 10 to the negative 3rd power in photon-energy range from 10 keV to greater than 10 MeV. In its spectroscopic aspect, instrument enables identification of both narrow and weak gamma-ray spectral peaks.

Atomistic three-dimensional coherent x-ray imaging of nonbiological systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ho, Phay J.; Knight, Chris; Tegze, Miklos

We computationally study the resolution limits for three-dimensional coherent x-ray diffractive imaging of heavy, nonbiological systems using Ar clusters as a prototype. We treat electronic and nuclear dynamics on an equal footing and remove the frozen-lattice approximation often used in electronic damage studies. We explore the achievable resolution as a function of pulse parameters (fluence level, pulse duration, and photon energy) and particle size. The contribution of combined lattice and electron dynamics is not negligible even for 2 fs pulses, and the Compton scattering is less deleterious than in biological systems for atomic-scale imaging. Although free-electron scattering represents a significantmore » background, we find that recovery of the original structure is in principle possible with 3 angstrom resolution for particles of 11 nm diameter.« less

Atomistic three-dimensional coherent x-ray imaging of nonbiological systems

DOE PAGES

Ho, Phay J.; Knight, Chris; Tegze, Miklos; ...

2016-12-12

We computationally study the resolution limits for three-dimensional coherent x-ray diffractive imaging of heavy, nonbiological systems using Ar clusters as a prototype. We treat electronic and nuclear dynamics on an equal footing and remove the frozen-lattice approximation often used in electronic damage studies. We explore the achievable resolution as a function of pulse parameters (fluence level, pulse duration, and photon energy) and particle size. The contribution of combined lattice and electron dynamics is not negligible even for 2 fs pulses, and the Compton scattering is less deleterious than in biological systems for atomic-scale imaging. Although free-electron scattering represents a significantmore » background, we find that recovery of the original structure is in principle possible with 3 angstrom resolution for particles of 11 nm diameter.« less

Three dimensional imaging detector employing wavelength-shifting optical fibers

DOEpatents

Worstell, William A.

1997-01-01

A novel detector element structure and method for its use is provided. In a preferred embodiment, one or more inorganic scintillating crystals are coupled through wavelength shifting optical fibers (WLSFs) to position sensitive photomultipliers (PS-PMTs). The superior detector configuration in accordance with this invention is designed for an array of applications in high spatial resolution gamma ray sensing with particular application to SPECT, PET and PVI imaging systems. The design provides better position resolution than prior art devices at a lower total cost. By employing wavelength shifting fibers (WLSFs), the sensor configuration of this invention can operate with a significant reduction in the number of photomultipliers and electronics channels, while potentially improving the resolution of the system by allowing three dimensional reconstruction of energy deposition positions.

Three dimensional imaging detector employing wavelength-shifting optical fibers

DOEpatents

Worstell, W.A.

1997-02-04

A novel detector element structure and method for its use is provided. In a preferred embodiment, one or more inorganic scintillating crystals are coupled through wavelength shifting optical fibers (WLSFs) to position sensitive photomultipliers (PS-PMTs). The superior detector configuration in accordance with this invention is designed for an array of applications in high spatial resolution gamma ray sensing with particular application to SPECT, PET and PVI imaging systems. The design provides better position resolution than prior art devices at a lower total cost. By employing wavelength shifting fibers (WLSFs), the sensor configuration of this invention can operate with a significant reduction in the number of photomultipliers and electronics channels, while potentially improving the resolution of the system by allowing three dimensional reconstruction of energy deposition positions. 11 figs.

Kinetic Simulations of Current-Sheet Formation and Reconnection at a Magnetic X Line

NASA Technical Reports Server (NTRS)

Black, C.; Antiochos, S. K.; Hesse, M.; Karpen, J. T.; DeVore, C. R.; Kuznetsova, M. M.; Zenitani, S.

2011-01-01

The integration of kinetic effects into macroscopic numerical models is currently of great interest to the plasma physics community, particularly in the context of magnetic reconnection. We are examining the formation and reconnection of current sheets in a simple, two-dimensional X-line configuration using high resolution particle-in-cell (PIC) simulations. The initial potential magnetic field is perturbed by thermal pressure introduced into the particle distribution far from the X line. The relaxation of this added stress leads to the development of a current sheet, which reconnects for imposed stress of sufficient strength. We compare the evolution and final state of our PIC simulations with magnetohydrodynamic simulations assuming both uniform and localized resistivities, and with force-free magnetic-field equilibria in which the amount of reconnect ion across the X line can be constrained to be zero (ideal evolution) or optimal (minimum final magnetic energy). We will discuss implications of our results for reconnection onset and cessation at kinetic scales in dynamically formed current sheets, such as those occurring in the terrestrial magnetotail and solar corona.

The ANTARES optical beacon system

NASA Astrophysics Data System (ADS)

Ageron, M.; Aguilar, J. A.; Albert, A.; Ameli, F.; Anghinolfi, M.; Anton, G.; Anvar, S.; Ardellier-Desages, F.; Aslanides, E.; Aubert, J.-J.; Auer, R.; Barbarito, E.; Basa, S.; Battaglieri, M.; Becherini, Y.; Beltramelli, J.; Bertin, V.; Bigi, A.; Billault, M.; Blaes, R.; de Botton, N.; Bouwhuis, M. C.; Bradbury, S. M.; Bruijn, R.; Brunner, J.; Burgio, G. F.; Busto, J.; Cafagna, F.; Caillat, L.; Calzas, A.; Capone, A.; Caponetto, L.; Carmona, E.; Carr, J.; Cartwright, S. L.; Castel, D.; Castorina, E.; Cavasinni, V.; Cecchini, S.; Ceres, A.; Charvis, P.; Chauchot, P.; Chiarusi, T.; Circella, M.; Colnard, C.; Compère, C.; Coniglione, R.; Cottini, N.; Coyle, P.; Cuneo, S.; Cussatlegras, A.-S.; Damy, G.; van Dantzig, R.; de Bonis, G.; de Marzo, C.; de Vita, R.; Dekeyser, I.; Delagnes, E.; Denans, D.; Deschamps, A.; Destelle, J.-J.; Dinkespieler, B.; Distefano, C.; Donzaud, C.; Drogou, J.-F.; Druillole, F.; Durand, D.; Ernenwein, J.-P.; Escoffier, S.; Falchini, E.; Favard, S.; Fehr, F.; Feinstein, F.; Ferry, S.; Fiorello, C.; Flaminio, V.; Fratini, K.; Fuda, J.-L.; Galeotti, S.; Gallone, J.-M.; Giacomelli, G.; Girard, N.; Gojak, C.; Goret, Ph.; Graf, K.; Hallewell, G.; Harakeh, M. N.; Hartmann, B.; Heijboer, A.; Heine, E.; Hello, Y.; Hernández-Rey, J. J.; Hößl, J.; Hoffman, C.; Hogenbirk, J.; Hubbard, J. R.; Jaquet, M.; Jaspers, M.; de Jong, M.; Jouvenot, F.; Kalantar-Nayestanaki, N.; Kappes, A.; Karg, T.; Katz, U.; Keller, P.; Kok, E.; Kok, H.; Kooijman, P.; Kopper, C.; Korolkova, E. V.; Kouchner, A.; Kretschmer, W.; Kruijer, A.; Kuch, S.; Kudryavstev, V. A.; Lagier, P.; Lahmann, R.; Lamanna, G.; Lamare, P.; Lambard, G.; Languillat, J.-C.; Laschinsky, H.; Lavalle, J.; Le Guen, Y.; Le Provost, H.; Le van Suu, A.; Lefèvre, D.; Legou, T.; Lelaizant, G.; Lim, G.; Lo Presti, D.; Loehner, H.; Loucatos, S.; Louis, F.; Lucarelli, F.; Lyashuk, V.; Marcelin, M.; Margiotta, A.; Masullo, R.; Mazéas, F.; Mazure, A.; McMillan, J. E.; Megna, R.; Melissas, M.; Migneco, E.; Milovanovic, A.; Mongelli, M.; Montaruli, T.; Morganti, M.; Moscoso, L.; Musumeci, M.; Naumann-Godo, M.; Naumann, C.; Niess, V.; Noble, T.; Olivetto, C.; Ostasch, R.; Palanque-Delabrouille, N.; Payre, P.; Peek, H.; Perez, A.; Petta, C.; Piattelli, P.; Pillet, R.; Pineau, J.-P.; Poinsignon, J.; Popa, V.; Pradier, T.; Racca, C.; Randazzo, N.; van Randwijk, J.; Real, D.; van Rens, B.; Réthoré, F.; Rewiersma, P.; Riccobene, G.; Rigaud, V.; Ripani, M.; Roca, V.; Roda, C.; Rolin, J. F.; Rose, H. J.; Rostovtsev, A.; Roux, J.; Ruppi, M.; Russo, G. V.; Rusydi, G.; Salesa, F.; Salomon, K.; Sapienza, P.; Schmitt, F.; Schuller, J.-P.; Shanidze, R.; Sokalski, I.; Spona, T.; Spurio, M.; van der Steenhoven, G.; Stolarczyk, T.; Streeb, K.; Sulak, L.; Taiuti, M.; Tamburini, C.; Tao, C.; Terreni, G.; Thompson, L. F.; Urbano, F.; Valdy, P.; Valente, V.; Vallage, B.; Vaudaine, G.; Venekamp, G.; Verlaat, B.; Vernin, P.; de Vries-Uiterweerd, G.; van Wijk, R.; Wijnker, G.; de Witt Huberts, P.; Wobbe, G.; de Wolf, E.; Yao, A.-F.; Zaborov, D.; Zaccone, H.; Zornoza, J. D.; Zúñiga, J.

2007-08-01

ANTARES is a neutrino telescope being deployed in the Mediterranean Sea. It consists of a three-dimensional array of photomultiplier tubes that can detect the Cherenkov light induced by charged particles produced in the interactions of neutrinos with the surrounding medium. High angular resolution can be achieved, in particular, when a muon is produced, provided that the Cherenkov photons are detected with sufficient timing precision. Considerations of the intrinsic time uncertainties stemming from the transit time spread in the photomultiplier tubes and the mechanism of transmission of light in sea water lead to the conclusion that a relative time accuracy of the order of 0.5 ns is desirable. Accordingly, different time calibration systems have been developed for the ANTARES telescope. In this article, a system based on Optical Beacons, a set of external and well-controlled pulsed light sources located throughout the detector, is described. This calibration system takes into account the optical properties of sea water, which is used as the detection volume of the ANTARES telescope. The design, tests, construction and first results of the two types of beacons, LED and laser-based, are presented.

Spatial studies of planetary nebulae with IRAS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hawkins, G.W.; Zuckerman, B.

1991-06-01

The infrared sizes at the four IRAS wavelengths of 57 planetaries, most with 20-60 arcsec optical size, are derived from spatial deconvolution of one-dimensional survey mode scans. Survey observations from multiple detectors and hours confirmed (HCON) observations are combined to increase the sampling to a rate that is sufficient for successful deconvolution. The Richardson-Lucy deconvolution algorithm is used to obtain an increase in resolution of a factor of about 2 or 3 from the normal IRAS detector sizes of 45, 45, 90, and 180 arcsec at wavelengths 12, 25, 60, and 100 microns. Most of the planetaries deconvolve at 12more » and 25 microns to sizes equal to or smaller than the optical size. Some of the planetaries with optical rings 60 arcsec or more in diameter show double-peaked IRAS profiles. Many, such as NGC 6720 and NGC 6543 show all infrared sizes equal to the optical size, while others indicate increasing infrared size with wavelength. Deconvolved IRAS profiles are presented for the 57 planetaries at nearly all wavelengths where IRAS flux densities are 1-2 Jy or higher. 60 refs.« less

Grid Convergence for Turbulent Flows(Invited)

NASA Technical Reports Server (NTRS)

Diskin, Boris; Thomas, James L.; Rumsey, Christopher L.; Schwoppe, Axel

2015-01-01

A detailed grid convergence study has been conducted to establish accurate reference solutions corresponding to the one-equation linear eddy-viscosity Spalart-Allmaras turbulence model for two dimensional turbulent flows around the NACA 0012 airfoil and a flat plate. The study involved three widely used codes, CFL3D (NASA), FUN3D (NASA), and TAU (DLR), and families of uniformly refined structured grids that differ in the grid density patterns. Solutions computed by different codes on different grid families appear to converge to the same continuous limit, but exhibit different convergence characteristics. The grid resolution in the vicinity of geometric singularities, such as a sharp trailing edge, is found to be the major factor affecting accuracy and convergence of discrete solutions, more prominent than differences in discretization schemes and/or grid elements. The results reported for these relatively simple turbulent flows demonstrate that CFL3D, FUN3D, and TAU solutions are very accurate on the finest grids used in the study, but even those grids are not sufficient to conclusively establish an asymptotic convergence order.

Integrating anatomy and function for zebrafish circuit analysis.

PubMed

Arrenberg, Aristides B; Driever, Wolfgang

2013-01-01

Due to its transparency, virtually every brain structure of the larval zebrafish is accessible to light-based interrogation of circuit function. Advanced stimulation techniques allow the activation of optogenetic actuators at different resolution levels, and genetically encoded calcium indicators report the activity of a large proportion of neurons in the CNS. Large datasets result and need to be analyzed to identify cells that have specific properties-e.g., activity correlation to sensory stimulation or behavior. Advances in three-dimensional (3D) functional mapping in zebrafish are promising; however, the mere coordinates of implicated neurons are not sufficient. To comprehensively understand circuit function, these functional maps need to be placed into the proper context of morphological features and projection patterns, neurotransmitter phenotypes, and key anatomical landmarks. We discuss the prospect of merging functional and anatomical data in an integrated atlas from the perspective of our work on long-range dopaminergic neuromodulation and the oculomotor system. We propose that such a resource would help researchers to surpass current hurdles in circuit analysis to achieve an integrated understanding of anatomy and function.

Entanglement Area Law in Disordered Free Fermion Anderson Model in One, Two, and Three Dimensions

DOE PAGES

Pouranvari, Mohammad; Zhang, Yuhui; Yang, Kun

2015-01-01

We calculate numerically the entanglement entropy of free fermion ground states in one-, two-, and three-dimensional Anderson models and find that it obeys the area law as long as the linear size of the subsystem is sufficiently larger than the mean free path. This result holds in the metallic phase of the three-dimensional Anderson model, where the mean free path is finite although the localization length is infinite. Relation between the present results and earlier ones on area law violation in special one-dimensional models that support metallic phases is discussed.

Entanglement Area Law in Disordered Free Fermion Anderson Model in One, Two, and Three Dimensions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pouranvari, Mohammad; Zhang, Yuhui; Yang, Kun

We calculate numerically the entanglement entropy of free fermion ground states in one-, two-, and three-dimensional Anderson models and find that it obeys the area law as long as the linear size of the subsystem is sufficiently larger than the mean free path. This result holds in the metallic phase of the three-dimensional Anderson model, where the mean free path is finite although the localization length is infinite. Relation between the present results and earlier ones on area law violation in special one-dimensional models that support metallic phases is discussed.

Optimized Deconvolution for Maximum Axial Resolution in Three-Dimensional Aberration-Corrected Scanning Transmission Electron Microscopy

PubMed Central

Ramachandra, Ranjan; de Jonge, Niels

2012-01-01

Three-dimensional (3D) data sets were recorded of gold nanoparticles placed on both sides of silicon nitride membranes using focal series aberration-corrected scanning transmission electron microscopy (STEM). The deconvolution of the 3D datasets was optimized to obtain the highest possible axial resolution. The deconvolution involved two different point spread function (PSF)s, each calculated iteratively via blind deconvolution.. Supporting membranes of different thicknesses were tested to study the effect of beam broadening on the deconvolution. It was found that several iterations of deconvolution was efficient in reducing the imaging noise. With an increasing number of iterations, the axial resolution was increased, and most of the structural information was preserved. Additional iterations improved the axial resolution by maximal a factor of 4 to 6, depending on the particular dataset, and up to 8 nm maximal, but at the cost of a reduction of the lateral size of the nanoparticles in the image. Thus, the deconvolution procedure optimized for highest axial resolution is best suited for applications where one is interested in the 3D locations of nanoparticles only. PMID:22152090

Rewritable ghost floating gates by tunnelling triboelectrification for two-dimensional electronics

PubMed Central

Kim, Seongsu; Kim, Tae Yun; Lee, Kang Hyuck; Kim, Tae-Ho; Cimini, Francesco Arturo; Kim, Sung Kyun; Hinchet, Ronan; Kim, Sang-Woo; Falconi, Christian

2017-01-01

Gates can electrostatically control charges inside two-dimensional materials. However, integrating independent gates typically requires depositing and patterning suitable insulators and conductors. Moreover, after manufacturing, gates are unchangeable. Here we introduce tunnelling triboelectrification for localizing electric charges in very close proximity of two-dimensional materials. As representative materials, we use chemical vapour deposition graphene deposited on a SiO2/Si substrate. The triboelectric charges, generated by friction with a Pt-coated atomic force microscope tip and injected through defects, are trapped at the air–SiO2 interface underneath graphene and act as ghost floating gates. Tunnelling triboelectrification uniquely permits to create, modify and destroy p and n regions at will with the spatial resolution of atomic force microscopes. As a proof of concept, we draw rewritable p/n+ and p/p+ junctions with resolutions as small as 200 nm. Our results open the way to time-variant two-dimensional electronics where conductors, p and n regions can be defined on demand. PMID:28649986

Rewritable ghost floating gates by tunnelling triboelectrification for two-dimensional electronics

NASA Astrophysics Data System (ADS)

Kim, Seongsu; Kim, Tae Yun; Lee, Kang Hyuck; Kim, Tae-Ho; Cimini, Francesco Arturo; Kim, Sung Kyun; Hinchet, Ronan; Kim, Sang-Woo; Falconi, Christian

2017-06-01

Gates can electrostatically control charges inside two-dimensional materials. However, integrating independent gates typically requires depositing and patterning suitable insulators and conductors. Moreover, after manufacturing, gates are unchangeable. Here we introduce tunnelling triboelectrification for localizing electric charges in very close proximity of two-dimensional materials. As representative materials, we use chemical vapour deposition graphene deposited on a SiO2/Si substrate. The triboelectric charges, generated by friction with a Pt-coated atomic force microscope tip and injected through defects, are trapped at the air-SiO2 interface underneath graphene and act as ghost floating gates. Tunnelling triboelectrification uniquely permits to create, modify and destroy p and n regions at will with the spatial resolution of atomic force microscopes. As a proof of concept, we draw rewritable p/n+ and p/p+ junctions with resolutions as small as 200 nm. Our results open the way to time-variant two-dimensional electronics where conductors, p and n regions can be defined on demand.

High-resolution echocardiography

NASA Technical Reports Server (NTRS)

Nathan, R.

1979-01-01

High resolution computer aided ultrasound system provides two-and three-dimensional images of beating heart from many angles. System provides means for determining whether small blood vessels around the heart are blocked or if heart wall is moving normally without interference of dead and noncontracting muscle tissue.

Three-dimensional passive sensing photon counting for object classification

NASA Astrophysics Data System (ADS)

Yeom, Seokwon; Javidi, Bahram; Watson, Edward

2007-04-01

In this keynote address, we address three-dimensional (3D) distortion-tolerant object recognition using photon-counting integral imaging (II). A photon-counting linear discriminant analysis (LDA) is discussed for classification of photon-limited images. We develop a compact distortion-tolerant recognition system based on the multiple-perspective imaging of II. Experimental and simulation results have shown that a low level of photons is sufficient to classify out-of-plane rotated objects.

Measurement of pO2 and pH in Living Breast Tumor Models with Three-Dimensional Resolution by Multiphoton Microscopy during Combined Therapy with Herceptin

DTIC Science & Technology

2007-04-01

contact with a freshly spin-coated NC–titania pre- polymer , which was transferred to a hot plate to initiate polymerization . The pattern of the PDMS stamp...to quantify pO2 and pH in vivo with high three-dimensional resolution (~1 µm3) and significant depth penetration (up to 400 µm) with MPLSM. The...proposed to develop techniques for measuring in vivo pO2 and pH of HER2-positive and negative primary tumors in murine models of breast cancer using

Real-time stereo generation for surgical vision during minimal invasive robotic surgery

NASA Astrophysics Data System (ADS)

Laddi, Amit; Bhardwaj, Vijay; Mahapatra, Prasant; Pankaj, Dinesh; Kumar, Amod

2016-03-01

This paper proposes a framework for 3D surgical vision for minimal invasive robotic surgery. It presents an approach for generating the three dimensional view of the in-vivo live surgical procedures from two images captured by very small sized, full resolution camera sensor rig. A pre-processing scheme is employed to enhance the image quality and equalizing the color profile of two images. Polarized Projection using interlacing two images give a smooth and strain free three dimensional view. The algorithm runs in real time with good speed at full HD resolution.

Wave field restoration using three-dimensional Fourier filtering method.

PubMed

Kawasaki, T; Takai, Y; Ikuta, T; Shimizu, R

2001-11-01

A wave field restoration method in transmission electron microscopy (TEM) was mathematically derived based on a three-dimensional (3D) image formation theory. Wave field restoration using this method together with spherical aberration correction was experimentally confirmed in through-focus images of amorphous tungsten thin film, and the resolution of the reconstructed phase image was successfully improved from the Scherzer resolution limit to the information limit. In an application of this method to a crystalline sample, the surface structure of Au(110) was observed in a profile-imaging mode. The processed phase image showed quantitatively the atomic relaxation of the topmost layer.

High-definition resolution three-dimensional imaging systems in laparoscopic radical prostatectomy: randomized comparative study with high-definition resolution two-dimensional systems.

PubMed

Kinoshita, Hidefumi; Nakagawa, Ken; Usui, Yukio; Iwamura, Masatsugu; Ito, Akihiro; Miyajima, Akira; Hoshi, Akio; Arai, Yoichi; Baba, Shiro; Matsuda, Tadashi

2015-08-01

Three-dimensional (3D) imaging systems have been introduced worldwide for surgical instrumentation. A difficulty of laparoscopic surgery involves converting two-dimensional (2D) images into 3D images and depth perception rearrangement. 3D imaging may remove the need for depth perception rearrangement and therefore have clinical benefits. We conducted a multicenter, open-label, randomized trial to compare the surgical outcome of 3D-high-definition (HD) resolution and 2D-HD imaging in laparoscopic radical prostatectomy (LRP), in order to determine whether an LRP under HD resolution 3D imaging is superior to that under HD resolution 2D imaging in perioperative outcome, feasibility, and fatigue. One-hundred twenty-two patients were randomly assigned to a 2D or 3D group. The primary outcome was time to perform vesicourethral anastomosis (VUA), which is technically demanding and may include a number of technical difficulties considered in laparoscopic surgeries. VUA time was not significantly shorter in the 3D group (26.7 min, mean) compared with the 2D group (30.1 min, mean) (p = 0.11, Student's t test). However, experienced surgeons and 3D-HD imaging were independent predictors for shorter VUA times (p = 0.000, p = 0.014, multivariate logistic regression analysis). Total pneumoperitoneum time was not different. No conversion case from 3D to 2D or LRP to open RP was observed. Fatigue was evaluated by a simulation sickness questionnaire and critical flicker frequency. Results were not different between the two groups. Subjective feasibility and satisfaction scores were significantly higher in the 3D group. Using a 3D imaging system in LRP may have only limited advantages in decreasing operation times over 2D imaging systems. However, the 3D system increased surgical feasibility and decreased surgeons' effort levels without inducing significant fatigue.

Recent approaches for bridging the pressure gap in photoelectron microspectroscopy

PubMed Central

Kolmakov, Andrei; Gregoratti, Luca; Kiskinova, Maya; Günther, Sebastian

2016-01-01

Ambient-pressure photoelectron spectroscopy (APPES) and microscopy are at the frontier of modern chemical analysis at liquid-gas, solid-liquid and solid-gas interfaces, bridging science and engineering of functional materials. Complementing the current state-of-the art of the instruments, we survey in this short review several alternative APPES approaches, developed recently in the scanning photoelectron microscope (SPEM) at the Elettra laboratory. In particular, we report on experimental setups for dynamic near-ambient pressure environment, using pulsed-gas injection in the vicinity of samples or reaction cells with very small apertures, allowing for experiments without introducing additional differential pumping stages. The major part of the review is dedicated to the construction and performance of novel environmental cells using ultrathin electron-transparent but molecularly impermeable membranes to isolate the gas or liquid ambient from the electron detector operating in ultra-high vacuum (UHV). We demonstrate that two dimensional materials, such as graphene and derivatives, are mechanically robust to withstand atmospheric - UHV pressure differences and are sufficiently transparent for the photoelectrons emitted from samples immersed in the liquid or gaseous media. There are many unique opportunities for APPES using X-rays over a wide energy range. We show representative results that illustrate the potential of these ‘ambient-pressure’ approaches. Combined with the ca 100 nm lateral resolution of SPEM, they can overcome the pressure gap challenges and address the evolution of chemical composition and electronic structure at surface and interfaces under realistic operation conditions with unprecedented lateral and spectral resolution. PMID:28008215

An FPGA-Based Real-Time Maximum Likelihood 3D Position Estimation for a Continuous Crystal PET Detector

NASA Astrophysics Data System (ADS)

Wang, Yonggang; Xiao, Yong; Cheng, Xinyi; Li, Deng; Wang, Liwei

2016-02-01

For the continuous crystal-based positron emission tomography (PET) detector built in our lab, a maximum likelihood algorithm adapted for implementation on a field programmable gate array (FPGA) is proposed to estimate the three-dimensional (3D) coordinate of interaction position with the single-end detected scintillation light response. The row-sum and column-sum readout scheme organizes the 64 channels of photomultiplier (PMT) into eight row signals and eight column signals to be readout for X- and Y-coordinates estimation independently. By the reference events irradiated in a known oblique angle, the probability density function (PDF) for each depth-of-interaction (DOI) segment is generated, by which the reference events in perpendicular irradiation are assigned to DOI segments for generating the PDFs for X and Y estimation in each DOI layer. Evaluated by the experimental data, the algorithm achieves an average X resolution of 1.69 mm along the central X-axis, and DOI resolution of 3.70 mm over the whole thickness (0-10 mm) of crystal. The performance improvements from 2D estimation to the 3D algorithm are also presented. Benefiting from abundant resources of FPGA and a hierarchical storage arrangement, the whole algorithm can be implemented into a middle-scale FPGA. By a parallel structure in pipelines, the 3D position estimator on the FPGA can achieve a processing throughput of 15 M events/s, which is sufficient for the requirement of real-time PET imaging.

Development of digital reconstructed radiography software at new treatment facility for carbon-ion beam scanning of National Institute of Radiological Sciences.

PubMed

Mori, Shinichiro; Inaniwa, Taku; Kumagai, Motoki; Kuwae, Tsunekazu; Matsuzaki, Yuka; Furukawa, Takuji; Shirai, Toshiyuki; Noda, Koji

2012-06-01

To increase the accuracy of carbon ion beam scanning therapy, we have developed a graphical user interface-based digitally-reconstructed radiograph (DRR) software system for use in routine clinical practice at our center. The DRR software is used in particular scenarios in the new treatment facility to achieve the same level of geometrical accuracy at the treatment as at the imaging session. DRR calculation is implemented simply as the summation of CT image voxel values along the X-ray projection ray. Since we implemented graphics processing unit-based computation, the DRR images are calculated with a speed sufficient for the particular clinical practice requirements. Since high spatial resolution flat panel detector (FPD) images should be registered to the reference DRR images in patient setup process in any scenarios, the DRR images also needs higher spatial resolution close to that of FPD images. To overcome the limitation of the CT spatial resolution imposed by the CT voxel size, we applied image processing to improve the calculated DRR spatial resolution. The DRR software introduced here enabled patient positioning with sufficient accuracy for the implementation of carbon-ion beam scanning therapy at our center.

High-resolution imaging gamma-ray spectroscopy with externally segmented germanium detectors

NASA Technical Reports Server (NTRS)

Callas, J. L.; Mahoney, W. A.; Varnell, L. S.; Wheaton, W. A.

1993-01-01

Externally segmented germanium detectors promise a breakthrough in gamma-ray imaging capabilities while retaining the superb energy resolution of germanium spectrometers. An angular resolution of 0.2 deg becomes practical by combining position-sensitive germanium detectors having a segment thickness of a few millimeters with a one-dimensional coded aperture located about a meter from the detectors. Correspondingly higher angular resolutions are possible with larger separations between the detectors and the coded aperture. Two-dimensional images can be obtained by rotating the instrument. Although the basic concept is similar to optical or X-ray coded-aperture imaging techniques, several complicating effects arise because of the penetrating nature of gamma rays. The complications include partial transmission through the coded aperture elements, Compton scattering in the germanium detectors, and high background count rates. Extensive electron-photon Monte Carlo modeling of a realistic detector/coded-aperture/collimator system has been performed. Results show that these complicating effects can be characterized and accounted for with no significant loss in instrument sensitivity.

Compressive light field imaging

NASA Astrophysics Data System (ADS)

Ashok, Amit; Neifeld, Mark A.

2010-04-01

Light field imagers such as the plenoptic and the integral imagers inherently measure projections of the four dimensional (4D) light field scalar function onto a two dimensional sensor and therefore, suffer from a spatial vs. angular resolution trade-off. Programmable light field imagers, proposed recently, overcome this spatioangular resolution trade-off and allow high-resolution capture of the (4D) light field function with multiple measurements at the cost of a longer exposure time. However, these light field imagers do not exploit the spatio-angular correlations inherent in the light fields of natural scenes and thus result in photon-inefficient measurements. Here, we describe two architectures for compressive light field imaging that require relatively few photon-efficient measurements to obtain a high-resolution estimate of the light field while reducing the overall exposure time. Our simulation study shows that, compressive light field imagers using the principal component (PC) measurement basis require four times fewer measurements and three times shorter exposure time compared to a conventional light field imager in order to achieve an equivalent light field reconstruction quality.

Three-dimensional laser microvision.

PubMed

Shimotahira, H; Iizuka, K; Chu, S C; Wah, C; Costen, F; Yoshikuni, Y

2001-04-10

A three-dimensional (3-D) optical imaging system offering high resolution in all three dimensions, requiring minimum manipulation and capable of real-time operation, is presented. The system derives its capabilities from use of the superstructure grating laser source in the implementation of a laser step frequency radar for depth information acquisition. A synthetic aperture radar technique was also used to further enhance its lateral resolution as well as extend the depth of focus. High-speed operation was made possible by a dual computer system consisting of a host and a remote microcomputer supported by a dual-channel Small Computer System Interface parallel data transfer system. The system is capable of operating near real time. The 3-D display of a tunneling diode, a microwave integrated circuit, and a see-through image taken by the system operating near real time are included. The depth resolution is 40 mum; lateral resolution with a synthetic aperture approach is a fraction of a micrometer and that without it is approximately 10 mum.

Advances in solar radio astronomy

NASA Technical Reports Server (NTRS)

Kundu, M. R.

1982-01-01

The status of the observations and interpretations of the sun's radio emission covering the entire radio spectrum from millimeter wavelengths to hectometer and kilometer wavelengths is reviewed. Emphasis is given to the progress made in solar radio physics as a result of recent advances in plasma and radiation theory. It is noted that the capability now exists of observing the sun with a spatial resolution of approximately a second of arc and a temporal resolution of about a millisecond at centimeter wavelengths and of obtaining fast multifrequency two-dimensional pictures of the sun at meter and decameter wavelengths. A summary is given of the properties of nonflaring active regions at millimeter, centimeter, and meter-decameter wavelengths. The properties of centimeter wave bursts are discussed in connection with the high spatial resolution observations. The observations of the preflare build-up of an active region are reviewed. High spatial resolution observations (a few seconds of arc to approximately 1 arcsec) are discussed, with particular attention given to the one- and two-dimensional maps of centimeter-wavelength burst sources.

Advances in high-resolution imaging--techniques for three-dimensional imaging of cellular structures.

PubMed

Lidke, Diane S; Lidke, Keith A

2012-06-01

A fundamental goal in biology is to determine how cellular organization is coupled to function. To achieve this goal, a better understanding of organelle composition and structure is needed. Although visualization of cellular organelles using fluorescence or electron microscopy (EM) has become a common tool for the cell biologist, recent advances are providing a clearer picture of the cell than ever before. In particular, advanced light-microscopy techniques are achieving resolutions below the diffraction limit and EM tomography provides high-resolution three-dimensional (3D) images of cellular structures. The ability to perform both fluorescence and electron microscopy on the same sample (correlative light and electron microscopy, CLEM) makes it possible to identify where a fluorescently labeled protein is located with respect to organelle structures visualized by EM. Here, we review the current state of the art in 3D biological imaging techniques with a focus on recent advances in electron microscopy and fluorescence super-resolution techniques.

Insight into resolution enhancement in generalized two-dimensional correlation spectroscopy.

PubMed

Ma, Lu; Sikirzhytski, Vitali; Hong, Zhenmin; Lednev, Igor K; Asher, Sanford A

2013-03-01

Generalized two-dimensional correlation spectroscopy (2D-COS) can be used to enhance spectral resolution in order to help differentiate highly overlapped spectral bands. Despite the numerous extensive 2D-COS investigations, the origin of the 2D spectral resolution enhancement mechanism(s) is not completely understood. In the work here, we studied the 2D-COS of simulated spectra in order to develop new insights into the dependence of 2D-COS spectral features on the overlapping band separations, their intensities and bandwidths, and their band intensity change rates. We found that the features in the 2D-COS maps that are derived from overlapping bands were determined by the spectral normalized half-intensities and the total intensity changes of the correlated bands. We identified the conditions required to resolve overlapping bands. In particular, 2D-COS peak resolution requires that the normalized half-intensities of a correlating band have amplitudes between the maxima and minima of the normalized half-intensities of the overlapping bands.

Insight into Resolution Enhancement in Generalized Two-Dimensional Correlation Spectroscopy

PubMed Central

Ma, Lu; Sikirzhytski, Vitali; Hong, Zhenmin; Lednev, Igor K.; Asher, Sanford A.

2014-01-01

Generalized two-dimensional correlation spectroscopy (2D COS) can be used to enhance spectral resolution in order to help differentiate highly overlapped spectral bands. Despite the numerous extensive 2D COS investigations, the origin of the 2D spectral resolution enhancement mechanism(s) are not completely understood. In the work here we studied the 2D COS of simulated spectra in order to develop new insights into the dependence of the 2D COS spectral features on the overlapping band separations, their intensities and bandwidths, and their band intensity change rates. We find that the features in the 2D COS maps that derive from overlapping bands are determined by the spectral normalized half-intensities and the total intensity changes of the correlated bands. We identify the conditions required to resolve overlapping bands. In particular, 2D COS peak resolution requires that the normalized half-intensities of a correlating band have amplitudes between the maxima and minima of the normalized half-intensities of the overlapping bands. PMID:23452492

Micro-Mirrors for Nanoscale Three-Dimensional Microscopy

PubMed Central

Seale, Kevin; Janetopoulos, Chris; Wikswo, John

2013-01-01

A research-grade optical microscope is capable of resolving fine structures in two-dimensional images. However, three-dimensional resolution, or the ability of the microscope to distinguish between objects lying above or below the focal plane from in-focus objects, is not nearly as good as in-plane resolution. In this issue of ACS Nano, McMahon et al. report the use of mirrored pyramidal wells with a conventional microscope for rapid, 3D localization and tracking of nanoparticles. Mirrors have been used in microscopy before, but recent work with MPWs is unique because it enables the rapid determination of the x-, y-, and z-position of freely diffusing nanoparticles and cellular nanostructures with unprecedented speed and spatial accuracy. As inexpensive tools for 3D visualization, mirrored pyramidal wells may prove to be invaluable aids in nanotechnology and engineering of nanomaterials. PMID:19309167

High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography

NASA Astrophysics Data System (ADS)

Kim, Kyoohyun; Yoon, HyeOk; Diez-Silva, Monica; Dao, Ming; Dasari, Ramachandra R.; Park, YongKeun

2014-01-01

We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated.

Puzzle Imaging: Using Large-Scale Dimensionality Reduction Algorithms for Localization.

PubMed

Glaser, Joshua I; Zamft, Bradley M; Church, George M; Kording, Konrad P

2015-01-01

Current high-resolution imaging techniques require an intact sample that preserves spatial relationships. We here present a novel approach, "puzzle imaging," that allows imaging a spatially scrambled sample. This technique takes many spatially disordered samples, and then pieces them back together using local properties embedded within the sample. We show that puzzle imaging can efficiently produce high-resolution images using dimensionality reduction algorithms. We demonstrate the theoretical capabilities of puzzle imaging in three biological scenarios, showing that (1) relatively precise 3-dimensional brain imaging is possible; (2) the physical structure of a neural network can often be recovered based only on the neural connectivity matrix; and (3) a chemical map could be reproduced using bacteria with chemosensitive DNA and conjugative transfer. The ability to reconstruct scrambled images promises to enable imaging based on DNA sequencing of homogenized tissue samples.

Two-dimensional mesh embedding for Galerkin B-spline methods

NASA Technical Reports Server (NTRS)

Shariff, Karim; Moser, Robert D.

1995-01-01

A number of advantages result from using B-splines as basis functions in a Galerkin method for solving partial differential equations. Among them are arbitrary order of accuracy and high resolution similar to that of compact schemes but without the aliasing error. This work develops another property, namely, the ability to treat semi-structured embedded or zonal meshes for two-dimensional geometries. This can drastically reduce the number of grid points in many applications. Both integer and non-integer refinement ratios are allowed. The report begins by developing an algorithm for choosing basis functions that yield the desired mesh resolution. These functions are suitable products of one-dimensional B-splines. Finally, test cases for linear scalar equations such as the Poisson and advection equation are presented. The scheme is conservative and has uniformly high order of accuracy throughout the domain.

High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography

PubMed Central

Kim, Kyoohyun; Yoon, HyeOk; Diez-Silva, Monica; Dao, Ming; Dasari, Ramachandra R.

2013-01-01

Abstract. We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated. PMID:23797986

Si3N4 Optomechanical Crystals in the Resolved-sideband Regime

DTIC Science & Technology

2014-01-27

between cavity photons and phonons was used to demon- strate electromagnetically induced transparency ( EIT ) mediated by a mechanical resonance; radiation...wavelength range. The sideband resolution achieved was sufficient for observing, at room temperature and atmos- pheric pressure, EIT mediated by a 4 GHz...sufficient for the observation of optomechanical EIT at room tempera- ture and atmospheric pressure. This effect corresponds to the creation of a narrow

40 CFR 92.127 - Emission measurement accuracy.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Emission measurement accuracy. (a) Good engineering practice dictates that exhaust emission sample analyzer... resolution read-out systems such as computers, data loggers, etc., can provide sufficient accuracy and...

40 CFR 92.127 - Emission measurement accuracy.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Emission measurement accuracy. (a) Good engineering practice dictates that exhaust emission sample analyzer... resolution read-out systems such as computers, data loggers, etc., can provide sufficient accuracy and...

40 CFR 92.127 - Emission measurement accuracy.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Emission measurement accuracy. (a) Good engineering practice dictates that exhaust emission sample analyzer... resolution read-out systems such as computers, data loggers, etc., can provide sufficient accuracy and...

40 CFR 92.127 - Emission measurement accuracy.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Emission measurement accuracy. (a) Good engineering practice dictates that exhaust emission sample analyzer... resolution read-out systems such as computers, data loggers, etc., can provide sufficient accuracy and...

40 CFR 92.127 - Emission measurement accuracy.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Emission measurement accuracy. (a) Good engineering practice dictates that exhaust emission sample analyzer... resolution read-out systems such as computers, data loggers, etc., can provide sufficient accuracy and...

Bose-Einstein condensation in chains with power-law hoppings: Exact mapping on the critical behavior in d-dimensional regular lattices.

PubMed

Dias, W S; Bertrand, D; Lyra, M L

2017-06-01

Recent experimental progress on the realization of quantum systems with highly controllable long-range interactions has impelled the study of quantum phase transitions in low-dimensional systems with power-law couplings. Long-range couplings mimic higher-dimensional effects in several physical contexts. Here, we provide the exact relation between the spectral dimension d at the band bottom and the exponent α that tunes the range of power-law hoppings of a one-dimensional ideal lattice Bose gas. We also develop a finite-size scaling analysis to obtain some relevant critical exponents and the critical temperature of the BEC transition. In particular, an irrelevant dangerous scaling field has to be taken into account when the hopping range is sufficiently large to make the effective dimensionality d>4.

Bose-Einstein condensation in chains with power-law hoppings: Exact mapping on the critical behavior in d -dimensional regular lattices

NASA Astrophysics Data System (ADS)

Dias, W. S.; Bertrand, D.; Lyra, M. L.

2017-06-01

Recent experimental progress on the realization of quantum systems with highly controllable long-range interactions has impelled the study of quantum phase transitions in low-dimensional systems with power-law couplings. Long-range couplings mimic higher-dimensional effects in several physical contexts. Here, we provide the exact relation between the spectral dimension d at the band bottom and the exponent α that tunes the range of power-law hoppings of a one-dimensional ideal lattice Bose gas. We also develop a finite-size scaling analysis to obtain some relevant critical exponents and the critical temperature of the BEC transition. In particular, an irrelevant dangerous scaling field has to be taken into account when the hopping range is sufficiently large to make the effective dimensionality d >4 .

Single-particle cryo-EM using alignment by classification (ABC): the structure of Lumbricus terrestris haemoglobin

PubMed Central

Seer-Linnemayr, Charlotte; Ravelli, Raimond B. G.; Matadeen, Rishi; De Carlo, Sacha; Alewijnse, Bart; Portugal, Rodrigo V.; Pannu, Navraj S.; Schatz, Michael; van Heel, Marin

2017-01-01

Single-particle cryogenic electron microscopy (cryo-EM) can now yield near-atomic resolution structures of biological complexes. However, the reference-based alignment algorithms commonly used in cryo-EM suffer from reference bias, limiting their applicability (also known as the ‘Einstein from random noise’ problem). Low-dose cryo-EM therefore requires robust and objective approaches to reveal the structural information contained in the extremely noisy data, especially when dealing with small structures. A reference-free pipeline is presented for obtaining near-atomic resolution three-dimensional reconstructions from heterogeneous (‘four-dimensional’) cryo-EM data sets. The methodologies integrated in this pipeline include a posteriori camera correction, movie-based full-data-set contrast transfer function determination, movie-alignment algorithms, (Fourier-space) multivariate statistical data compression and unsupervised classification, ‘random-startup’ three-dimensional reconstructions, four-dimensional structural refinements and Fourier shell correlation criteria for evaluating anisotropic resolution. The procedures exclusively use information emerging from the data set itself, without external ‘starting models’. Euler-angle assignments are performed by angular reconstitution rather than by the inherently slower projection-matching approaches. The comprehensive ‘ABC-4D’ pipeline is based on the two-dimensional reference-free ‘alignment by classification’ (ABC) approach, where similar images in similar orientations are grouped by unsupervised classification. Some fundamental differences between X-ray crystallography versus single-particle cryo-EM data collection and data processing are discussed. The structure of the giant haemoglobin from Lumbricus terrestris at a global resolution of ∼3.8 Å is presented as an example of the use of the ABC-4D procedure. PMID:28989723

Parallel Computation and Visualization of Three-dimensional, Time-dependent, Thermal Convective Flows

NASA Technical Reports Server (NTRS)

Wang, P.; Li, P.

1998-01-01

A high-resolution numerical study on parallel systems is reported on three-dimensional, time-dependent, thermal convective flows. A parallel implentation on the finite volume method with a multigrid scheme is discussed, and a parallel visualization systemm is developed on distributed systems for visualizing the flow.

High resolution, two-dimensional imaging, microchannel plate detector for use on a sounding rocket experiment

NASA Technical Reports Server (NTRS)

Bush, Brett C.; Cotton, Daniel M.; Siegmund, Oswald H.; Chakrabarti, Supriya; Harris, Walter; Clarke, John

1991-01-01

We discuss a high resolution microchannel plate (MCP) imaging detector to be used in measurements of Doppler-shifted hydrogen Lyman-alpha line emission from Jupiter and the interplanetary medium. The detector is housed in a vacuum-tight stainless steel cylinder (to provide shielding from magnetic fields) with a MgF2 window. Operating at nominal voltage, the four plate configuration provides a gain of 1.2 x 10 exp 7 electrons per incident photon. The wedge-and-strip anode has two-dimensional imaging capabilities, with a resolution of 40 microns FWHM over a one centimeter diameter area. The detector has a high quantum efficiency while retaining a low background rate. A KBr photocathode is used to enhance the quantum efficiency of the bare MCPs to a value of 35 percent at Lyman-alpha.

Lateral resolution testing of a novel developed confocal microscopic imaging system

NASA Astrophysics Data System (ADS)

Zhang, Xin; Zhang, Yunhai; Chang, Jian; Huang, Wei; Xue, Xiaojun; Xiao, Yun

2015-10-01

Laser scanning confocal microscope has been widely used in biology, medicine and material science owing to its advantages of high resolution and tomographic imaging. Based on a set of confirmatory experiments and system design, a novel confocal microscopic imaging system is developed. The system is composed of a conventional fluorescence microscope and a confocal scanning unit. In the scanning unit a laser beam coupling module provides four different wavelengths 405nm 488nm 561nm and 638nm which can excite a variety of dyes. The system works in spot-to-spot scanning mode with a two-dimensional galvanometer. A 50 microns pinhole is used to guarantee that stray light is blocked and only the fluorescence signal from the focal point can be received . The three-channel spectral splitter is used to perform fluorescence imaging at three different working wavelengths simultaneously. The rat kidney tissue slice is imaged using the developed confocal microscopic imaging system. Nucleues labeled by DAPI and kidney spherule curved pipe labeled by Alexa Fluor 488 can be imaged clearly and respectively, realizing the distinction between the different components of mouse kidney tissue. The three-dimensional tomographic imaging of mouse kidney tissue is reconstructed by several two-dimensional images obtained in different depths. At last the resolution of the confocal microscopic imaging system is tested quantitatively. The experimental result shows that the system can achieve lateral resolution priority to 230nm.

High spatial resolution measurements in a single stage ram accelerator

NASA Technical Reports Server (NTRS)

Hinkey, J. B.; Burnham, E. A.; Bruckner, A. P.

1992-01-01

High spatial resolution experimental tube wall pressure measurements of ram accelerator gas dynamic phenomena are presented in this paper. The ram accelerator is a ramjet-in-tube device which operates in a manner similar to that of a conventional ramjet. The projectile resembles the centerbody of a ramjet and travels supersonically through a tube filled with a combustible gaseous mixture, with the tube acting as the outer cowling. Pressure data are recorded as the projectile passes by sensors mounted in the tube wall at various locations along the tube. Utilization of special highly instrumented sections of tube has allowed the recording of gas dynamic phenomena with high resolution. High spatial resolution tube wall pressure data from the three regimes of propulsion studied to date (subdetonative, transdetonative, and superdetonative) in a single stage gas mixture are presented and reveal the three-dimensional character of the flow field induced by projectile fins and the canting of the fins and the canting of the projectile body relative to the tube wall. Also presented for comparison to the experimental data are calculations made with an inviscid, three-dimensional CFD code. The knowledge gained from these experiments and simulations is useful in understanding the underlying nature of ram accelerator propulsive regimes, as well as assisting in the validation of three-dimensional CFD coded which model unsteady, chemically reactive flows.

Genome U-Plot: a whole genome visualization.

PubMed

Gaitatzes, Athanasios; Johnson, Sarah H; Smadbeck, James B; Vasmatzis, George

2018-05-15

The ability to produce and analyze whole genome sequencing (WGS) data from samples with structural variations (SV) generated the need to visualize such abnormalities in simplified plots. Conventional two-dimensional representations of WGS data frequently use either circular or linear layouts. There are several diverse advantages regarding both these representations, but their major disadvantage is that they do not use the two-dimensional space very efficiently. We propose a layout, termed the Genome U-Plot, which spreads the chromosomes on a two-dimensional surface and essentially quadruples the spatial resolution. We present the Genome U-Plot for producing clear and intuitive graphs that allows researchers to generate novel insights and hypotheses by visualizing SVs such as deletions, amplifications, and chromoanagenesis events. The main features of the Genome U-Plot are its layered layout, its high spatial resolution and its improved aesthetic qualities. We compare conventional visualization schemas with the Genome U-Plot using visualization metrics such as number of line crossings and crossing angle resolution measures. Based on our metrics, we improve the readability of the resulting graph by at least 2-fold, making apparent important features and making it easy to identify important genomic changes. A whole genome visualization tool with high spatial resolution and improved aesthetic qualities. An implementation and documentation of the Genome U-Plot is publicly available at https://github.com/gaitat/GenomeUPlot. vasmatzis.george@mayo.edu. Supplementary data are available at Bioinformatics online.

Crash Padding Research : Vol. I. Material Mechanical Properties.

DOT National Transportation Integrated Search

1986-08-01

The dynamic mechanical properties of Uniroyal Ensolite AAC, a viscoelastic closed-cell foam rubber, are investigated by means of materials tests. Sufficient test data is presented to form a basis for one-dimensional (uniform compression) empirical co...

Hypersonic ignition and thrust production in a scramjet

NASA Technical Reports Server (NTRS)

Paull, A.

1993-01-01

Experimental results are given for the specific impulse produced by a two-dimensional scramjet at flight speeds ranging between 2.5 and 5.5 km/s with a combustion chamber Mach number of 4.5. Both hydrogen and ethane fuels were used. Results show that provided sufficiently high pressures and sufficiently long combustion chambers are used specific impulses in excess of 1500 s can be obtained with hydrogen. Ethane produced specific impulses less than 600 s with the same conditions and model configuration.

On controllability of homogeneous and inhomogeneous discrete-time multi-input bilinear systems in dimension two

NASA Astrophysics Data System (ADS)

Tie, Lin

2017-08-01

In this paper, the controllability problem of two-dimensional discrete-time multi-input bilinear systems is completely solved. The homogeneous and the inhomogeneous cases are studied separately and necessary and sufficient conditions for controllability are established by using a linear algebraic method, which are easy to apply. Moreover, for the uncontrollable systems, near-controllability is considered and similar necessary and sufficient conditions are also obtained. Finally, examples are provided to demonstrate the results of this paper.

Poisson traces, D-modules, and symplectic resolutions

NASA Astrophysics Data System (ADS)

Etingof, Pavel; Schedler, Travis

2018-03-01

We survey the theory of Poisson traces (or zeroth Poisson homology) developed by the authors in a series of recent papers. The goal is to understand this subtle invariant of (singular) Poisson varieties, conditions for it to be finite-dimensional, its relationship to the geometry and topology of symplectic resolutions, and its applications to quantizations. The main technique is the study of a canonical D-module on the variety. In the case the variety has finitely many symplectic leaves (such as for symplectic singularities and Hamiltonian reductions of symplectic vector spaces by reductive groups), the D-module is holonomic, and hence, the space of Poisson traces is finite-dimensional. As an application, there are finitely many irreducible finite-dimensional representations of every quantization of the variety. Conjecturally, the D-module is the pushforward of the canonical D-module under every symplectic resolution of singularities, which implies that the space of Poisson traces is dual to the top cohomology of the resolution. We explain many examples where the conjecture is proved, such as symmetric powers of du Val singularities and symplectic surfaces and Slodowy slices in the nilpotent cone of a semisimple Lie algebra. We compute the D-module in the case of surfaces with isolated singularities and show it is not always semisimple. We also explain generalizations to arbitrary Lie algebras of vector fields, connections to the Bernstein-Sato polynomial, relations to two-variable special polynomials such as Kostka polynomials and Tutte polynomials, and a conjectural relationship with deformations of symplectic resolutions. In the appendix we give a brief recollection of the theory of D-modules on singular varieties that we require.

Poisson traces, D-modules, and symplectic resolutions.

PubMed

Etingof, Pavel; Schedler, Travis

2018-01-01

We survey the theory of Poisson traces (or zeroth Poisson homology) developed by the authors in a series of recent papers. The goal is to understand this subtle invariant of (singular) Poisson varieties, conditions for it to be finite-dimensional, its relationship to the geometry and topology of symplectic resolutions, and its applications to quantizations. The main technique is the study of a canonical D-module on the variety. In the case the variety has finitely many symplectic leaves (such as for symplectic singularities and Hamiltonian reductions of symplectic vector spaces by reductive groups), the D-module is holonomic, and hence, the space of Poisson traces is finite-dimensional. As an application, there are finitely many irreducible finite-dimensional representations of every quantization of the variety. Conjecturally, the D-module is the pushforward of the canonical D-module under every symplectic resolution of singularities, which implies that the space of Poisson traces is dual to the top cohomology of the resolution. We explain many examples where the conjecture is proved, such as symmetric powers of du Val singularities and symplectic surfaces and Slodowy slices in the nilpotent cone of a semisimple Lie algebra. We compute the D-module in the case of surfaces with isolated singularities and show it is not always semisimple. We also explain generalizations to arbitrary Lie algebras of vector fields, connections to the Bernstein-Sato polynomial, relations to two-variable special polynomials such as Kostka polynomials and Tutte polynomials, and a conjectural relationship with deformations of symplectic resolutions. In the appendix we give a brief recollection of the theory of D-modules on singular varieties that we require.

Pushing the limits of high-resolution functional MRI using a simple high-density multi-element coil design.

PubMed

Petridou, N; Italiaander, M; van de Bank, B L; Siero, J C W; Luijten, P R; Klomp, D W J

2013-01-01

Recent studies have shown that functional MRI (fMRI) can be sensitive to the laminar and columnar organization of the cortex based on differences in the spatial and temporal characteristics of the blood oxygenation level-dependent (BOLD) signal originating from the macrovasculature and the neuronal-specific microvasculature. Human fMRI studies at this scale of the cortical architecture, however, are very rare because the high spatial/temporal resolution required to explore these properties of the BOLD signal are limited by the signal-to-noise ratio. Here, we show that it is possible to detect BOLD signal changes at an isotropic spatial resolution as high as 0.55 mm at 7 T using a high-density multi-element surface coil with minimal electronics, which allows close proximity to the head. The coil comprises of very small, 1 × 2-cm(2) , elements arranged in four flexible modules of four elements each (16-channel) that can be positioned within 1 mm from the head. As a result of this proximity, tissue losses were five-fold greater than coil losses and sufficient to exclude preamplifier decoupling. When compared with a standard 16-channel head coil, the BOLD sensitivity was approximately 2.2-fold higher for a high spatial/temporal resolution (1 mm isotropic/0.4 s), multi-slice, echo planar acquisition, and approximately three- and six-fold higher for three-dimensional echo planar images acquired with isotropic resolutions of 0.7 and 0.55 mm, respectively. Improvements in parallel imaging performance (geometry factor) were up to around 1.5-fold with increasing acceleration factor, and improvements in fMRI detectability (temporal signal-to-noise ratio) were up to around four-fold depending on the distance to the coil. Although deeper lying structures may not benefit from the design, most fMRI questions pertain to the neocortex which lies within approximately 4 cm from the surface. These results suggest that the resolution of fMRI (at 7 T) can approximate levels that are closer to the spatial/temporal scale of the fundamental functional organization of the human cortex using a simple high-density coil design for high sensitivity. Copyright © 2012 John Wiley & Sons, Ltd.

Nonlinear Analysis of Cavitating Propellers in Nonuniform Flow

DTIC Science & Technology

1992-10-16

Helmholtz more than a century ago [4]. The method was later extended to treat curved bodies at zero cavitation number by Levi - Civita [4]. The theory was...122, 1895. [63] M.P. Tulin. Steady two -dimensional cavity flows about slender bodies . Technical Report 834, DTMB, May 1953. [64] M.P. Tulin...iterative solution for two -dimensional flows is remarkably fast and that the accuracy of the first iteration solution is sufficient for a wide range of

Constructing space difference schemes which satisfy a cell entropy inequality

NASA Technical Reports Server (NTRS)

Merriam, Marshal L.

1989-01-01

A numerical methodology for solving convection problems is presented, using finite difference schemes which satisfy the second law of thermodynamics on a cell-by-cell basis in addition to the usual conservation laws. It is shown that satisfaction of a cell entropy inequality is sufficient, in some cases, to guarantee nonlinear stability. Some details are given for several one-dimensional problems, including the quasi-one-dimensional Euler equations applied to flow in a nozzle.

High resolution MR microscopy

NASA Astrophysics Data System (ADS)

Ciobanu, Luisa

Magnetic resonance imaging (MRI) microscopy [1] has the potential to bring the full capabilities of NMR to arbitrarily specified localized positions within small samples. The most interesting target of study is the living biological cell, with typical dimensions ˜100 mum, but with substructures that are much smaller, such as the cell nucleus (typically ˜10 mu m) and mitochondria (1--10 mum). One anticipates that the development of MR microscopy with resolution at the level of these substructures or better and with a wide, three dimensional field-of-view could open a new avenue of investigation into the biology of the living cell. Although the first MR image of a single biological cell was reported in 1987 [2], the cell imaged had quite large (˜1 mm diameter) spatial dimensions and the resolution obtained (on the order of 10 mu m) was not adequate for meaningful imaging of more typically sized cells. The quest for higher resolution has continued. In 1989 Zhou et al. [3] obtained fully three dimensional images with spatial resolution of (6.37 mum)3, or 260 femtoliters. While better "in-plane" resolutions (i.e., the resolution in 2 of the 3 spatial dimensions) have since been obtained, [4, 5] this volume resolution was not exceeded until quite recently by Lee et al., [6] who report 2D images having volume resolution of 75 mum 3 and in-plane resolution of 1 mum. In parallel with these advances in raw resolution several investigators [7, 8, 9] have focused on localized spectroscopy and/or chemical shift imaging. The key obstacles to overcome in MR microscopy are (1) the loss of signal to noise that occurs when observing small volumes and (2) molecular diffusion during the measurement or encoding. To date the problem of sensitivity has typically been addressed by employing small micro-coil receivers. [10] The problem of molecular diffusion can only be defeated with strong magnetic field gradients that can encode spatial information quickly. We report MR microscopy images on phantoms [11, 12] and biological samples (paramecia, algae, brain tissue, lipidic mesophases) obtained using using magnetic field gradients as large as 50 Tesla/meter (5000 G/cm) [13] and micro-coils [14]. Images have voxel resolution as high as (3.7 mum by 3.3 mum by 3.3 mum), or 41 mu m3 (41 femtoliters, containing 2.7 x 10 12 proton spins) [12], marginally the highest voxel resolution reported to date. They are also fully three dimensional, with wide fields of view.

Laboratory-size three-dimensional water-window x-ray microscope with Wolter type I mirror optics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ohsuka, Shinji; The Graduate School for the Creation of New Photonics Industries, 1955-1 Kurematsu-cho, Nishi-ku, Hamamatsu-City, 431-1202; Ohba, Akira

2016-01-28

We constructed a laboratory-size three-dimensional water-window x-ray microscope that combines wide-field transmission x-ray microscopy with tomographic reconstruction techniques. It consists of an electron-impact x-ray source emitting oxygen Kα x-rays, Wolter type I grazing incidence mirror optics, and a back-illuminated CCD for x-ray imaging. A spatial resolution limit better than 1.0 line pairs per micrometer was obtained for two-dimensional transmission images, and 1-μm-scale three-dimensional fine structures were resolved.

National Defense Center of Excellence for Industrial Metrology and 3D Imaging

DTIC Science & Technology

2012-10-18

validation rather than mundane data-reduction/analysis tasks. Indeed, the new financial and technical resources being brought to bear by integrating CT...of extremely fast axial scanners. By replacing the single-spot detector by a detector array, a three-dimensional image is acquired by one depth scan...the number of acquired voxels per complete two-dimensional or three-dimensional image, the axial and lateral resolution, the depth range, the

A long-time, high spatiotemporal resolution optical recording system for membrane potential activity via real-time writing to the hard disk.

PubMed

Hirota, Akihiko; Ito, Shin-ichi

2006-06-01

Using real-time hard disk recording, we have developed an optical system for the long-duration detection of changes in membrane potential from 1,020 sites with a high temporal resolution. The signal-to-noise ratio was sufficient for analyzing the spreading pattern of excitatory waves in frog atria in a single sweep.

The Three-Dimensional Point Spread Function of Aberration-Corrected Scanning Transmission Electron Microscopy

PubMed Central

Lupini, A.R.; de Jonge, N.

2012-01-01

Aberration-correction reduces the depth of field in scanning transmission electron microscopy (STEM) and thus allows three-dimensional imaging by depth-sectioning. This imaging mode offers the potential for sub-Ångstrom lateral resolution and nanometer-scale depth sensitivity. For biological samples, which may be many microns across and where high lateral resolution may not always be needed, optimizing the depth resolution even at the expense of lateral resolution may be desired, aiming to image through thick specimens. Although there has been extensive work examining and optimizing the probe formation in two-dimensions, there is less known about the probe shape along the optical axis. Here the probe shape is examined in three-dimensions in an attempt to better understand the depth-resolution in this mode. Examples are presented of how aberrations change the probe shape in three-dimensions, and it is found that off-axial aberrations may need to be considered for focal series of large areas. It is shown that oversized or annular apertures theoretically improve the vertical resolution for 3D imaging of nanoparticles. When imaging nanoparticles of several nanometer size, regular STEM can thereby be optimized such that the vertical full width at half maximum approaches that of the aberration corrected STEM with a standard aperture. PMID:21878149

Super-resolution photoacoustic microscopy using a localized near-field of a plasmonic nanoaperture: a three-dimensional simulation study

NASA Astrophysics Data System (ADS)

Park, Byullee; Lee, Hongki; Upputuri, Paul Kumar; Pramanik, Manojit; Kim, Donghyun; Kim, Chulhong

2018-02-01

Super-resolution microscopy has been increasingly important to delineate nanoscale biological structures or nanoparticles. With these increasing demands, several imaging modalities, including super-resolution fluorescence microscope (SRFM) and electron microscope (EM), have been developed and commercialized. These modalities achieve nanoscale resolution, however, SRFM cannot image without fluorescence, and sample preparation of EM is not suitable for biological specimens. To overcome those disadvantages, we have numerically studied the possibility of superresolution photoacoustic microscopy (SR-PAM) based on near-field localization of light. Photoacoustic (PA) signal is generally acquired based on optical absorption contrast; thus it requires no agents or pre-processing for the samples. The lateral resolution of the conventional photoacoustic microscopy is limited to 200 nm by diffraction limit, therefore reducing the lateral resolution is a major research impetus. Our approach to breaking resolution limit is to use laser pulses of extremely small spot size as a light source. In this research, we simulated the PA signal by constructing the three dimensional SR-PAM system environment using the k-Wave toolbox. As the light source, we simulated ultrashort light pulses using geometrical nanoaperture with near-field localization of surface plasmons. Through the PA simulation, we have successfully distinguish cuboids spaced 3 nm apart. In the near future, we will develop the SR-PAM and it will contribute to biomedical and material sciences.

Castable three-dimensional stationary phase for electric field-driven applications

DOEpatents

Shepodd, Timothy J.; Whinnery, Jr., Leroy; Even, Jr., William R.

2005-01-25

A polymer material useful as the porous dielectric medium for microfluidic devices generally and electrokinetic pumps in particular. The polymer material is produced from an inverse (water-in-oil) emulsion that creates a 3-dimensional network characterized by small pores and high internal volume, characteristics that are particularly desirable for the dielectric medium for electrokinetic pumps. Further, the material can be cast-to-shape inside a microchannel. The use of bifunctional monomers provides for charge density within the polymer structure sufficient to support electroosmotic flow. The 3-dimensional polymeric material can also be covalently bound to the channel walls thereby making it suitable for high-pressure applications.

Castable three-dimensional stationary phase for electric field-driven applications

DOEpatents

Shepodd, Timothy J [Livermore, CA; Whinnery, Jr., Leroy; Even, Jr., William R.

2009-02-10

A polymer material useful as the porous dielectric medium for microfluidic devices generally and electrokinetic pumps in particular. The polymer material is produced from an inverse (water-in-oil) emulsion that creates a 3-dimensional network characterized by small pores and high internal volume, characteristics that are particularly desirable for the dielectric medium for electrokinetic pumps. Further, the material can be cast-to-shape inside a microchannel. The use of bifunctional monomers provides for charge density within the polymer structure sufficient to support electroosmotic flow. The 3-dimensional polymeric material can also be covalently bound to the channel walls thereby making it suitable for high-pressure applications.

Minimum-fuel, 3-dimensional flightpath guidance of transfer jets

NASA Technical Reports Server (NTRS)

Neuman, F.; Kreindler, E.

1984-01-01

Minimum fuel, three dimensional flightpaths for commercial jet aircraft are discussed. The theoretical development is divided into two sections. In both sections, the necessary conditions of optimal control, including singular arcs and state constraints, are used. One section treats the initial and final portions (below 10,000 ft) of long optimal flightpaths. Here all possible paths can be derived by generating fields of extremals. Another section treats the complete intermediate length, three dimensional terminal area flightpaths. Here only representative sample flightpaths can be computed. Sufficient detail is provided to give the student of optimal control a complex example of a useful application of optimal control theory.

A mechanism for hot-spot generation in a reactive two-dimensional sheared viscous layer

NASA Astrophysics Data System (ADS)

Timms, Robert; Purvis, Richard; Curtis, John P.

2018-05-01

A two-dimensional model for the non-uniform melting of a thin sheared viscous layer is developed. An asymptotic solution is presented for both a non-reactive and a reactive material. It is shown that the melt front is linearly stable to small perturbations in the non-reactive case, but becomes linearly unstable upon introduction of an Arrhenius source term to model the chemical reaction. Results demonstrate that non-uniform melting acts as a mechanism to generate hot spots that are found to be sufficient to reduce the time to ignition when compared with the corresponding one-dimensional model of melting.

Super-resolution links vinculin localization to function in focal adhesions.

PubMed

Giannone, Grégory

2015-07-01

Integrin-based focal adhesions integrate biochemical and biomechanical signals from the extracellular matrix and the actin cytoskeleton. The combination of three-dimensional super-resolution imaging and loss- or gain-of-function protein mutants now links the nanoscale dynamic localization of proteins to their activation and function within focal adhesions.

Science with a selfie stick: Plant biomass estimation using smartphone based ‘Structure From Motion’ photogrammetry

USDA-ARS?s Scientific Manuscript database

Significant advancements in photogrammetric Structure-from-Motion (SfM) software, coupled with improvements in the quality and resolution of smartphone cameras, has made it possible to create ultra-fine resolution three-dimensional models of physical objects using an ordinary smartphone. Here we pre...

Two-dimensional fluorescence spectroscopy of uranium isotopes in femtosecond laser ablation plumes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Phillips, Mark C.; Brumfield, Brian E.; LaHaye, Nicole

Here, we demonstrate measurement of uranium isotopes in femtosecond laser ablation plumes using two-dimensional fluorescence spectroscopy (2DFS). The high-resolution, tunable CW-laser spectroscopy technique clearly distinguishes atomic absorption from 235U and 238U in natural and highly enriched uranium metal samples. We present analysis of spectral resolution and analytical performance of 2DFS as a function of ambient pressure. Simultaneous measurement using time-resolved absorption spectroscopy provides information on temporal dynamics of the laser ablation plume and saturation behavior of fluorescence signals. The rapid, non-contact measurement is promising for in-field, standoff measurements of uranium enrichment for nuclear safety and security.

Two-dimensional fluorescence spectroscopy of uranium isotopes in femtosecond laser ablation plumes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Phillips, Mark C.; Brumfield, Brian E.; LaHaye, Nicole L.

We demonstrate measurement of uranium isotopes in femtosecond laser ablation plumes using two-dimensional fluorescence spectroscopy (2DFS). The high-resolution, tunable CW-laser spectroscopy technique clearly distinguishes atomic absorption from 235U and 238U in natural and highly enriched uranium metal samples. We present analysis of spectral resolution and analytical performance of 2DFS as a function of ambient pressure. Simultaneous measurement using time-resolved absorption spectroscopy provides information on temporal dynamics of the laser ablation plume and saturation behavior of fluorescence signals. The rapid, non-contact measurement is promising for in-field, standoff measurements of uranium enrichment for nuclear safety and security applications.

In situ two-dimensional imaging quick-scanning XAFS with pixel array detector.

PubMed

Tanida, Hajime; Yamashige, Hisao; Orikasa, Yuki; Oishi, Masatsugu; Takanashi, Yu; Fujimoto, Takahiro; Sato, Kenji; Takamatsu, Daiko; Murayama, Haruno; Arai, Hajime; Matsubara, Eiichiro; Uchimoto, Yoshiharu; Ogumi, Zempachi

2011-11-01

Quick-scanning X-ray absorption fine structure (XAFS) measurements were performed in transmission mode using a PILATUS 100K pixel array detector (PAD). The method can display a two-dimensional image for a large area of the order of a centimetre with a spatial resolution of 0.2 mm at each energy point in the XAFS spectrum. The time resolution of the quick-scanning method ranged from 10 s to 1 min per spectrum depending on the energy range. The PAD has a wide dynamic range and low noise, so the obtained spectra have a good signal-to-noise ratio.

Multispectral high-resolution hologram generation using orthographic projection images

NASA Astrophysics Data System (ADS)

Muniraj, I.; Guo, C.; Sheridan, J. T.

2016-08-01

We present a new method of synthesizing a digital hologram of three-dimensional (3D) real-world objects from multiple orthographic projection images (OPI). A high-resolution multiple perspectives of 3D objects (i.e., two dimensional elemental image array) are captured under incoherent white light using synthetic aperture integral imaging (SAII) technique and their OPIs are obtained respectively. The reference beam is then multiplied with the corresponding OPI and integrated to form a Fourier hologram. Eventually, a modified phase retrieval algorithm (GS/HIO) is applied to reconstruct the hologram. The principle is validated experimentally and the results support the feasibility of the proposed method.

Two-dimensional fluorescence spectroscopy of uranium isotopes in femtosecond laser ablation plumes

DOE PAGES

Phillips, Mark C.; Brumfield, Brian E.; LaHaye, Nicole; ...

2017-06-19

Here, we demonstrate measurement of uranium isotopes in femtosecond laser ablation plumes using two-dimensional fluorescence spectroscopy (2DFS). The high-resolution, tunable CW-laser spectroscopy technique clearly distinguishes atomic absorption from 235U and 238U in natural and highly enriched uranium metal samples. We present analysis of spectral resolution and analytical performance of 2DFS as a function of ambient pressure. Simultaneous measurement using time-resolved absorption spectroscopy provides information on temporal dynamics of the laser ablation plume and saturation behavior of fluorescence signals. The rapid, non-contact measurement is promising for in-field, standoff measurements of uranium enrichment for nuclear safety and security.

A High-Resolution, Three-Dimensional Model of Jupiter's Great Red Spot

NASA Technical Reports Server (NTRS)

Cho, James Y.-K.; delaTorreJuarez, Manuel; Ingersoll, Andrew P.; Dritschel, David G.

2001-01-01

The turbulent flow at the periphery of the Great Red Spot (GRS) contains many fine-scale filamentary structures, while the more quiescent core, bounded by a narrow high- velocity ring, exhibits organized, possibly counterrotating, motion. Past studies have neither been able to capture this complexity nor adequately study the effect of vertical stratification L(sub R)(zeta) on the GRS. We present results from a series of high-resolution, three-dimensional simulations that advect the dynamical tracer, potential vorticity. The detailed flow is successfully captured with a characteristic value of L(sub R) approx. equals 2000 km, independent of the precise vertical stratification profile.

Advanced Scintillator Detectors for Neutron Imaging in Inertial Confinement Fusion

NASA Astrophysics Data System (ADS)

Geppert-Kleinrath, Verena; Danly, Christopher; Merrill, Frank; Simpson, Raspberry; Volegov, Petr; Wilde, Carl

2016-10-01

The neutron imaging team at Los Alamos National Laboratory (LANL) has been providing two-dimensional neutron imaging of the inertial confinement fusion process at the National Ignition Facility (NIF) for over five years. Neutron imaging is a powerful tool in which position-sensitive detectors register neutrons emitted in the fusion reactions, producing a picture of the burning fuel. Recent images have revealed possible multi-dimensional asymmetries, calling for additional views to facilitate three-dimensional imaging. These will be along shorter lines of sight to stay within the existing facility at NIF. In order to field imaging capabilities equivalent to the existing system several technological challenges have to be met: high spatial resolution, high light output, and fast scintillator response to capture lower-energy neutrons, which have scattered from non-burning regions of fuel. Deuterated scintillators are a promising candidate to achieve the timing and resolution required; a systematic study of deuterated and non-deuterated polystyrene and liquid samples is currently ongoing. A test stand has been implemented to measure the response function, and preliminary data on resolution and light output have been obtained at the LANL Weapons Neutrons Research facility.

Electron ptychographic phase imaging of light elements in crystalline materials using Wigner distribution deconvolution

DOE PAGES

Yang, Hao; MacLaren, Ian; Jones, Lewys; ...

2017-04-01

Recent development in fast pixelated detector technology has allowed a two dimensional diffraction pattern to be recorded at every probe position of a two dimensional raster scan in a scanning transmission electron microscope (STEM), forming an information-rich four dimensional (4D) dataset. Electron ptychography has been shown to enable efficient coherent phase imaging of weakly scattering objects from a 4D dataset recorded using a focused electron probe, which is optimised for simultaneous incoherent Z-contrast imaging and spectroscopy in STEM. Thus coherent phase contrast and incoherent Z-contrast imaging modes can be efficiently combined to provide a good sensitivity of both light andmore » heavy elements at atomic resolution. Here, we explore the application of electron ptychography for atomic resolution imaging of strongly scattering crystalline specimens, and present experiments on imaging crystalline specimens including samples containing defects, under dynamical channelling conditions using an aberration corrected microscope. A ptychographic reconstruction method called Wigner distribution deconvolution (WDD) was implemented. Our experimental results and simulation results suggest that ptychography provides a readily interpretable phase image and great sensitivity for imaging light elements at atomic resolution in relatively thin crystalline materials.« less

Preparation of wholemount mouse intestine for high-resolution three-dimensional imaging using two-photon microscopy.

PubMed

Appleton, P L; Quyn, A J; Swift, S; Näthke, I

2009-05-01

Visualizing overall tissue architecture in three dimensions is fundamental for validating and integrating biochemical, cell biological and visual data from less complex systems such as cultured cells. Here, we describe a method to generate high-resolution three-dimensional image data of intact mouse gut tissue. Regions of highest interest lie between 50 and 200 mum within this tissue. The quality and usefulness of three-dimensional image data of tissue with such depth is limited owing to problems associated with scattered light, photobleaching and spherical aberration. Furthermore, the highest-quality oil-immersion lenses are designed to work at a maximum distance of

Fractal Dimensionality of Pore and Grain Volume of a Siliciclastic Marine Sand

NASA Astrophysics Data System (ADS)

Reed, A. H.; Pandey, R. B.; Lavoie, D. L.

Three-dimensional (3D) spatial distributions of pore and grain volumes were determined from high-resolution computer tomography (CT) images of resin-impregnated marine sands. Using a linear gradient extrapolation method, cubic three-dimensional samples were constructed from two-dimensional CT images. Image porosity (0.37) was found to be consistent with the estimate of porosity by water weight loss technique (0.36). Scaling of the pore volume (Vp) with the linear size (L), V~LD provides the fractal dimensionalities of the pore volume (D=2.74+/-0.02) and grain volume (D=2.90+/-0.02) typical for sedimentary materials.

A two-dimensional kinematic dynamo model of the ionospheric magnetic field at Venus

NASA Technical Reports Server (NTRS)

Cravens, T. E.; Wu, D.; Shinagawa, H.

1990-01-01

The results of a high-resolution, two-dimensional, time dependent, kinematic dynamo model of the ionospheric magnetic field of Venus are presented. Various one-dimensional models are considered and the two-dimensional model is then detailed. In this model, the two-dimensional magnetic induction equation, the magnetic diffusion-convection equation, is numerically solved using specified plasma velocities. Origins of the vertical velocity profile and of the horizontal velocities are discussed. It is argued that the basic features of the vertical magnetic field profile remain unaltered by horizontal flow effects and also that horizontal plasma flow can strongly affect the magnetic field for altitudes above 300 km.

A High Resolution Scale-of-four

DOE R&D Accomplishments Database

Fitch, V.

1949-08-25

A high resolution scale-of-four has been developed to be used in conjunction with the nuclear particle detection devices in applications where the counting rate is unusually high. Specifically, it is intended to precede the commercially available medium resolution scaling circuits and so decrease the resolving time of the counting system. The circuit will function reliably on continuously recurring pulses separated by less than 0.1 microseconds. It will resolve two pulses (occurring at a moderate repetition rate) which are spaced at 0.04 microseconds. A five-volt input signal is sufficient to actuate the device.

Three-dimensional structure of Erwinia carotovora L-asparaginase

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kislitsyn, Yu. A.; Kravchenko, O. V.; Nikonov, S. V.

2006-10-15

Three-dimensional structure of Erwinia carotovora L-asparaginase, which has antitumor activity and is used for the treatment of acute lymphoblastic leukemia, was solved at 3 A resolution and refined to R{sub cryst} = 20% and R{sub free} = 28%. Crystals of recombinant Erwinia carotovora L-asparaginase were grown by the hanging-drop vapor-diffusion method from protein solutions in a HEPES buffer (pH 6.5) and PEG MME 5000 solutions in a cacodylate buffer (pH 6.5) as the precipitant. Three-dimensional X-ray diffraction data were collected up to 3 A resolution from one crystal at room temperature. The structure was solved by the molecular replacement methodmore » using the coordinates of Erwinia chrysanthemi L-asparaginase as the starting model. The coordinates refined with the use of the CNS program package were deposited in the Protein Data Bank (PDB code 1ZCF)« less

High-resolution three-dimensional imaging radar

NASA Technical Reports Server (NTRS)

Cooper, Ken B. (Inventor); Chattopadhyay, Goutam (Inventor); Siegel, Peter H. (Inventor); Dengler, Robert J. (Inventor); Schlecht, Erich T. (Inventor); Mehdi, Imran (Inventor); Skalare, Anders J. (Inventor)

2010-01-01

A three-dimensional imaging radar operating at high frequency e.g., 670 GHz, is disclosed. The active target illumination inherent in radar solves the problem of low signal power and narrow-band detection by using submillimeter heterodyne mixer receivers. A submillimeter imaging radar may use low phase-noise synthesizers and a fast chirper to generate a frequency-modulated continuous-wave (FMCW) waveform. Three-dimensional images are generated through range information derived for each pixel scanned over a target. A peak finding algorithm may be used in processing for each pixel to differentiate material layers of the target. Improved focusing is achieved through a compensation signal sampled from a point source calibration target and applied to received signals from active targets prior to FFT-based range compression to extract and display high-resolution target images. Such an imaging radar has particular application in detecting concealed weapons or contraband.

Puzzle Imaging: Using Large-Scale Dimensionality Reduction Algorithms for Localization

PubMed Central

Glaser, Joshua I.; Zamft, Bradley M.; Church, George M.; Kording, Konrad P.

2015-01-01

Current high-resolution imaging techniques require an intact sample that preserves spatial relationships. We here present a novel approach, “puzzle imaging,” that allows imaging a spatially scrambled sample. This technique takes many spatially disordered samples, and then pieces them back together using local properties embedded within the sample. We show that puzzle imaging can efficiently produce high-resolution images using dimensionality reduction algorithms. We demonstrate the theoretical capabilities of puzzle imaging in three biological scenarios, showing that (1) relatively precise 3-dimensional brain imaging is possible; (2) the physical structure of a neural network can often be recovered based only on the neural connectivity matrix; and (3) a chemical map could be reproduced using bacteria with chemosensitive DNA and conjugative transfer. The ability to reconstruct scrambled images promises to enable imaging based on DNA sequencing of homogenized tissue samples. PMID:26192446

Deflection Measurements of a Thermally Simulated Nuclear Core Using a High-Resolution CCD-Camera

NASA Technical Reports Server (NTRS)

Stanojev, B. J.; Houts, M.

2004-01-01

Space fission systems under consideration for near-term missions all use compact. fast-spectrum reactor cores. Reactor dimensional change with increasing temperature, which affects neutron leakage. is the dominant source of reactivity feedback in these systems. Accurately measuring core dimensional changes during realistic non-nuclear testing is therefore necessary in predicting the system nuclear equivalent behavior. This paper discusses one key technique being evaluated for measuring such changes. The proposed technique is to use a Charged Couple Device (CCD) sensor to obtain deformation readings of electrically heated prototypic reactor core geometry. This paper introduces a technique by which a single high spatial resolution CCD camera is used to measure core deformation in Real-Time (RT). Initial system checkout results are presented along with a discussion on how additional cameras could be used to achieve a three- dimensional deformation profile of the core during test.

High-resolution neutron diffraction study of CuNCN: New evidence of structure anomalies at low temperature

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jacobs, Philipp; Houben, Andreas; Dronskowski, Richard, E-mail: drons@HAL9000.ac.rwth-aachen.de

Copper carbodiimide (CuNCN) is the nitrogen-containing analogue of cupric oxide. Based on high-resolution neutron-diffraction data, CuNCN's lattice parameters are derived as a function of the temperature. In accordance with a recent synchrotron study, a clear trend in the cell parameter a is observed accompanying the changing magnetic behavior. With decreasing temperature, a slowly decreases to a minimum at ∼100 K after which it rises again. The same trend—albeit more pronounced—is observed for the c lattice parameter at ∼35 K. The herein presented neutron powder-diffraction data also support the conjectured sequence of transitions from the high-temperature one-dimensional resonating valence-bond (RVB) statemore » to a transient two-dimensional RVB state and eventually, at lowest temperatures, into another two-dimensional RVB state, presumably the ground state.« less

Overview of electron crystallography of membrane proteins: crystallization and screening strategies using negative stain electron microscopy.

PubMed

Nannenga, Brent L; Iadanza, Matthew G; Vollmar, Breanna S; Gonen, Tamir

2013-01-01

Electron cryomicroscopy, or cryoEM, is an emerging technique for studying the three-dimensional structures of proteins and large macromolecular machines. Electron crystallography is a branch of cryoEM in which structures of proteins can be studied at resolutions that rival those achieved by X-ray crystallography. Electron crystallography employs two-dimensional crystals of a membrane protein embedded within a lipid bilayer. The key to a successful electron crystallographic experiment is the crystallization, or reconstitution, of the protein of interest. This unit describes ways in which protein can be expressed, purified, and reconstituted into well-ordered two-dimensional crystals. A protocol is also provided for negative stain electron microscopy as a tool for screening crystallization trials. When large and well-ordered crystals are obtained, the structures of both protein and its surrounding membrane can be determined to atomic resolution.

A Three-Dimensional Microdisplacement Sensing System Based on MEMS Bulk-Silicon Technology

PubMed Central

Wu, Junjie; Lei, Lihua; Chen, Xin; Cai, Xiaoyu; Li, Yuan; Han, Tao

2014-01-01

For the dimensional measurement and characterization of microsized and nanosized components, a three-dimensional microdisplacement sensing system was developed using the piezoresistive effect in silicon. The sensor was fabricated using microelectromechanical system bulk-silicon technology, and it was validated using the finite element method. A precise data acquisition circuit with an accuracy of 20 μV was designed to obtain weak voltage signals. By calibration, the sensing system was shown to have a sensitivity of 17.29 mV/μm and 4.59 mV/μm in the axial and lateral directions, respectively; the nonlinearity in these directions was 0.8% and 1.0% full scale, respectively. A full range of 4.6 μm was achieved in the axial direction. Results of a resolution test indicated that the sensing system had a resolution of 5 nm in the axial direction and 10 nm in the lateral direction. PMID:25360581

Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography.

PubMed

Yu, Young-Sang; Farmand, Maryam; Kim, Chunjoong; Liu, Yijin; Grey, Clare P; Strobridge, Fiona C; Tyliszczak, Tolek; Celestre, Rich; Denes, Peter; Joseph, John; Krishnan, Harinarayan; Maia, Filipe R N C; Kilcoyne, A L David; Marchesini, Stefano; Leite, Talita Perciano Costa; Warwick, Tony; Padmore, Howard; Cabana, Jordi; Shapiro, David A

2018-03-02

Battery function is determined by the efficiency and reversibility of the electrochemical phase transformations at solid electrodes. The microscopic tools available to study the chemical states of matter with the required spatial resolution and chemical specificity are intrinsically limited when studying complex architectures by their reliance on two-dimensional projections of thick material. Here, we report the development of soft X-ray ptychographic tomography, which resolves chemical states in three dimensions at 11 nm spatial resolution. We study an ensemble of nano-plates of lithium iron phosphate extracted from a battery electrode at 50% state of charge. Using a set of nanoscale tomograms, we quantify the electrochemical state and resolve phase boundaries throughout the volume of individual nanoparticles. These observations reveal multiple reaction points, intra-particle heterogeneity, and size effects that highlight the importance of multi-dimensional analytical tools in providing novel insight to the design of the next generation of high-performance devices.

Ultra-high resolution computed tomography imaging

DOEpatents

Paulus, Michael J.; Sari-Sarraf, Hamed; Tobin, Jr., Kenneth William; Gleason, Shaun S.; Thomas, Jr., Clarence E.

2002-01-01

A method for ultra-high resolution computed tomography imaging, comprising the steps of: focusing a high energy particle beam, for example x-rays or gamma-rays, onto a target object; acquiring a 2-dimensional projection data set representative of the target object; generating a corrected projection data set by applying a deconvolution algorithm, having an experimentally determined a transfer function, to the 2-dimensional data set; storing the corrected projection data set; incrementally rotating the target object through an angle of approximately 180.degree., and after each the incremental rotation, repeating the radiating, acquiring, generating and storing steps; and, after the rotating step, applying a cone-beam algorithm, for example a modified tomographic reconstruction algorithm, to the corrected projection data sets to generate a 3-dimensional image. The size of the spot focus of the beam is reduced to not greater than approximately 1 micron, and even to not greater than approximately 0.5 microns.

3D fluorescence anisotropy imaging using selective plane illumination microscopy.

PubMed

Hedde, Per Niklas; Ranjit, Suman; Gratton, Enrico

2015-08-24

Fluorescence anisotropy imaging is a popular method to visualize changes in organization and conformation of biomolecules within cells and tissues. In such an experiment, depolarization effects resulting from differences in orientation, proximity and rotational mobility of fluorescently labeled molecules are probed with high spatial resolution. Fluorescence anisotropy is typically imaged using laser scanning and epifluorescence-based approaches. Unfortunately, those techniques are limited in either axial resolution, image acquisition speed, or by photobleaching. In the last decade, however, selective plane illumination microscopy has emerged as the preferred choice for three-dimensional time lapse imaging combining axial sectioning capability with fast, camera-based image acquisition, and minimal light exposure. We demonstrate how selective plane illumination microscopy can be utilized for three-dimensional fluorescence anisotropy imaging of live cells. We further examined the formation of focal adhesions by three-dimensional time lapse anisotropy imaging of CHO-K1 cells expressing an EGFP-paxillin fusion protein.

Food Self-Sufficiency across scales: How local can we go?

NASA Astrophysics Data System (ADS)

Pradhan, Prajal; Lüdeke, Matthias K. B.; Reusser, Dominik E.; Kropp, Jürgen P.

2013-04-01

"Think global, act local" is a phrase often used in sustainability debates. Here, we explore the potential of regions to go for local supply in context of sustainable food consumption considering both the present state and the plausible future scenarios. We analyze data on the gridded crop calories production, the gridded livestock calories production, the gridded feed calories use and the gridded food calories consumption in 5' resolution. We derived these gridded data from various sources: Global Agro-ecological Zone (GAEZ v3.0), Gridded Livestock of the World (GLW), FAOSTAT, and Global Rural-Urban Mapping Project (GRUMP). For scenarios analysis, we considered changes in population, dietary patterns and possibility of obtaining the maximum potential yield. We investigate the food self-sufficiency multiple spatial scales. We start from the 5' resolution (i.e. around 10 km x 10 km in the equator) and look at 8 levels of aggregation ranging from the plausible lowest administrative level to the continental level. Results for the different spatial scales show that about 1.9 billion people live in the area of 5' resolution where enough calories can be produced to sustain their food consumption and the feed used. On the country level, about 4.4 billion population can be sustained without international food trade. For about 1 billion population from Asia and Africa, there is a need for cross-continental food trade. However, if we were able to achieve the maximum potential crop yield, about 2.6 billion population can be sustained within their living area of 5' resolution. Furthermore, Africa and Asia could be food self-sufficient by achieving their maximum potential crop yield and only round 630 million populations would be dependent on the international food trade. However, the food self-sufficiency status might differ under consideration of the future change in population, dietary patterns and climatic conditions. We provide an initial approach for investigating the regional and the local potential to address food security across multiple spatial scales. We identify the areas where one can depend more on local/regional products as a transition path towards sustainable consumption and production.

3-dimensional imaging at nanometer resolutions

DOEpatents

Werner, James H.; Goodwin, Peter M.; Shreve, Andrew P.

2010-03-09

An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.

A simplified hardwood log-sawing program for three-dimensional profile data

Treesearch

R. Edward Thomas

2011-01-01

Current laser scanning systems in sawmills collect low-resolution three-dimensional (3D) profiles of logs. However, these scanners are capable of much more. As a demonstration, the U.S. Forest Service, Forestry Sciences Laboratory in Princeton, WV, constructed a 3D laser log scanner using off -the-shelf industrial scanning components.

Multi-photon microfabrication of three-dimensional capillary-scale vascular networks

NASA Astrophysics Data System (ADS)

Skylar-Scott, Mark A.; Liu, Man-Chi; Wu, Yuelong; Yanik, Mehmet Fatih

2017-02-01

Biomimetic models of microvasculature could enable assays of complex cellular behavior at the capillary-level, and enable efficient nutrient perfusion for the maintenance of tissues. However, existing three-dimensional printing methods for generating perfusable microvasculature with have insufficient resolution to recapitulate the microscale geometry of capillaries. Here, we present a collection of multiphoton microfabrication methods that enable the production of precise, three-dimensional, branched microvascular networks in collagen. When endothelial cells are added to the channels, they form perfusable lumens with diameters as small as 10 μm. Using a similar photochemistry, we also demonstrate the micropatterning of proteins embedded in microfabricated collagen scaffolds, producing hybrid scaffolds with both defined microarchitecture with integrated gradients of chemical cues. We provide examples for how these hybrid microfabricated scaffolds could be used in angiogenesis and cell homing assays. Finally, we describe a new method for increasing the micropatterning speed by synchronous laser and stage scanning. Using these technologies, we are working towards large-scale (>1 cm), high resolution ( 1 μm) scaffolds with both microarchitecture and embedded protein cues, with applications in three-dimensional assays of cellular behavior.

[Using 2-DCOS to identify the molecular spectrum peaks for the isomer in the multi-component mixture gases Fourier transform infrared analysis].

PubMed

Zhao, An-Xin; Tang, Xiao-Jun; Zhang, Zhong-Hua; Liu, Jun-Hua

2014-10-01

The generalized two-dimensional correlation spectroscopy and Fourier transform infrared were used to identify hydrocarbon isomers in the mixed gases for absorption spectra resolution enhancement. The Fourier transform infrared spectrum of n-butane and iso-butane and the two-dimensional correlation infrared spectrum of concentration perturbation were used for analysis as an example. The all band and the main absorption peak wavelengths of Fourier transform infrared spectrum for single component gas showed that the spectra are similar, and if they were mixed together, absorption peaks overlap and peak is difficult to identify. The synchronous and asynchronous spectrum of two-dimensional correlation spectrum can clearly identify the iso-butane and normal butane and their respective characteristic absorption peak intensity. Iso-butane has strong absorption characteristics spectrum lines at 2,893, 2,954 and 2,893 cm(-1), and n-butane at 2,895 and 2,965 cm(-1). The analysis result in this paper preliminary verified that the two-dimensional infrared correlation spectroscopy can be used for resolution enhancement in Fourier transform infrared spectrum quantitative analysis.

Multi-Band Light Curves from Two-Dimensional Simulations of Gamma-Ray Burst Afterglows

NASA Astrophysics Data System (ADS)

MacFadyen, Andrew

2010-01-01

The dynamics of gamma-ray burst outflows is inherently multi-dimensional. 1.) We present high resolution two-dimensional relativistic hydrodynamics simulations of GRBs in the afterglow phase using adaptive mesh refinement (AMR). Using standard synchrotron radiation models, we compute multi-band light curves, from the radio to X-ray, directly from the 2D hydrodynamics simulation data. We will present on-axis light curves for both constant density and wind media. We will also present off-axis light curves relevant for searches for orphan afterglows. We find that jet breaks are smoothed due to both off-axis viewing and wind media effects. 2.) Non-thermal radiation mechanisms in GRB afterglows require substantial magnetic field strengths. In turbulence driven by shear instabilities in relativistic magnetized gas, we demonstrate that magnetic field is naturally amplified to half a percent of the total energy (epsilon B = 0.005). We will show high resolution three dimensional relativistic MHD simulations of this process as well as particle in cell (PIC) simulations of mildly relativistic collisionless shocks.

Atherosclerosis of the carotid artery: evaluation by magnetic resonance angiography.

PubMed

Wildy, K S; Yuan, C; Tsuruda, J S; Ferguson, M S; Wen, N; Subramaniam, D S; Strandness, D E

1996-01-01

Carotid artery atherosclerotic plaques (APs) can lead to brain ischemia, an event shown to correlate with both the degree of stenosis and the composition of the AP. Currently, accurate estimates of stenosis can be obtained by either x-ray angiography or three-dimensional time-of-flight (TOF) magnetic resonance angiography (MRA). Our purpose was to determine whether three-dimensional TOF MRA images could also provide information on plaque location, morphology, and composition. Seven pre-endarterectomy patients underwent three-dimensional TOF MRA. After endarterectomy, plaque histology was evaluated. Three-dimensional TOF MRA images contained sufficient soft tissue contrast to differentiate the plaques from the surrounding tissues in all cases. Estimation of plaque morphology had 80% correlation with histology. Finally, intraplaque hemorrhage and calcification were deplicted as regions of moderately high and very low intensity, respectively. These preliminary results suggest that three-dimensional TOF MRA may be useful in studying the development and progression of carotid atherosclerosis.

The Goertler vortex instability mechanism in three-dimensional boundary layers

NASA Technical Reports Server (NTRS)

Hall, P.

1984-01-01

The two dimensional boundary layer on a concave wall is centrifugally unstable with respect to vortices aligned with the basic flow for sufficiently high values of the Goertler number. However, in most situations of practical interest the basic flow is three dimensional and previous theoretical investigations do not apply. The linear stability of the flow over an infinitely long swept wall of variable curvature is considered. If there is no pressure gradient in the boundary layer the instability problem can always be related to an equivalent two dimensional calculation. However, in general, this is not the case and even for small values of the crossflow velocity field dramatic differences between the two and three dimensional problems emerge. When the size of the crossflow is further increased, the vortices in the neutral location have their axes locally perpendicular to the vortex lines of the basic flow.

Discovering Hidden Controlling Parameters using Data Analytics and Dimensional Analysis

NASA Astrophysics Data System (ADS)

Del Rosario, Zachary; Lee, Minyong; Iaccarino, Gianluca

2017-11-01

Dimensional Analysis is a powerful tool, one which takes a priori information and produces important simplifications. However, if this a priori information - the list of relevant parameters - is missing a relevant quantity, then the conclusions from Dimensional Analysis will be incorrect. In this work, we present novel conclusions in Dimensional Analysis, which provide a means to detect this failure mode of missing or hidden parameters. These results are based on a restated form of the Buckingham Pi theorem that reveals a ridge function structure underlying all dimensionless physical laws. We leverage this structure by constructing a hypothesis test based on sufficient dimension reduction, allowing for an experimental data-driven detection of hidden parameters. Both theory and examples will be presented, using classical turbulent pipe flow as the working example. Keywords: experimental techniques, dimensional analysis, lurking variables, hidden parameters, buckingham pi, data analysis. First author supported by the NSF GRFP under Grant Number DGE-114747.

4-D photoacoustic tomography.

PubMed

Xiang, Liangzhong; Wang, Bo; Ji, Lijun; Jiang, Huabei

2013-01-01

Photoacoustic tomography (PAT) offers three-dimensional (3D) structural and functional imaging of living biological tissue with label-free, optical absorption contrast. These attributes lend PAT imaging to a wide variety of applications in clinical medicine and preclinical research. Despite advances in live animal imaging with PAT, there is still a need for 3D imaging at centimeter depths in real-time. We report the development of four dimensional (4D) PAT, which integrates time resolutions with 3D spatial resolution, obtained using spherical arrays of ultrasonic detectors. The 4D PAT technique generates motion pictures of imaged tissue, enabling real time tracking of dynamic physiological and pathological processes at hundred micrometer-millisecond resolutions. The 4D PAT technique is used here to image needle-based drug delivery and pharmacokinetics. We also use this technique to monitor 1) fast hemodynamic changes during inter-ictal epileptic seizures and 2) temperature variations during tumor thermal therapy.

Electron crystallography of PhoE porin, an outer membrane, channel- forming protein from E. coli

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walian, P.J.

1989-11-01

One approach to studying the structure of membrane proteins is the use of electron crystallography. Dr. Bing Jap has crystallized PhoE pore-forming protein (porin) from the outer membrane of escherichia coli (E. coli) into monolayer crystals. The findings of this research and those of Jap (1988, 1989) have determined these crystals to be highly ordered, yielding structural information to a resolution of better than 2.8 angstroms. The task of this thesis has been to collect and process the electron diffraction patterns necessary to generate a complete three-dimensional set of high resolution structure factor amplitudes of PhoE porin. Fourier processing ofmore » these amplitudes when combined with the corresponding phase data is expected to yield the three-dimensional structure of PhoE porin at better than 3.5 angstroms resolution. 92 refs., 33 figs., 3 tabs. (CBS)« less

A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing.

PubMed

Murakami, Tatsuya C; Mano, Tomoyuki; Saikawa, Shu; Horiguchi, Shuhei A; Shigeta, Daichi; Baba, Kousuke; Sekiya, Hiroshi; Shimizu, Yoshihiro; Tanaka, Kenji F; Kiyonari, Hiroshi; Iino, Masamitsu; Mochizuki, Hideki; Tainaka, Kazuki; Ueda, Hiroki R

2018-04-01

A three-dimensional single-cell-resolution mammalian brain atlas will accelerate systems-level identification and analysis of cellular circuits underlying various brain functions. However, its construction requires efficient subcellular-resolution imaging throughout the entire brain. To address this challenge, we developed a fluorescent-protein-compatible, whole-organ clearing and homogeneous expansion protocol based on an aqueous chemical solution (CUBIC-X). The expanded, well-cleared brain enabled us to construct a point-based mouse brain atlas with single-cell annotation (CUBIC-Atlas). CUBIC-Atlas reflects inhomogeneous whole-brain development, revealing a significant decrease in the cerebral visual and somatosensory cortical areas during postnatal development. Probabilistic activity mapping of pharmacologically stimulated Arc-dVenus reporter mouse brains onto CUBIC-Atlas revealed the existence of distinct functional structures in the hippocampal dentate gyrus. CUBIC-Atlas is shareable by an open-source web-based viewer, providing a new platform for whole-brain cell profiling.

Hydrogel-Tissue Chemistry: Principles and Applications.

PubMed

Gradinaru, Viviana; Treweek, Jennifer; Overton, Kristin; Deisseroth, Karl

2018-05-20

Over the past five years, a rapidly developing experimental approach has enabled high-resolution and high-content information retrieval from intact multicellular animal (metazoan) systems. New chemical and physical forms are created in the hydrogel-tissue chemistry process, and the retention and retrieval of crucial phenotypic information regarding constituent cells and molecules (and their joint interrelationships) are thereby enabled. For example, rich data sets defining both single-cell-resolution gene expression and single-cell-resolution activity during behavior can now be collected while still preserving information on three-dimensional positioning and/or brain-wide wiring of those very same neurons-even within vertebrate brains. This new approach and its variants, as applied to neuroscience, are beginning to illuminate the fundamental cellular and chemical representations of sensation, cognition, and action. More generally, reimagining metazoans as metareactants-or positionally defined three-dimensional graphs of constituent chemicals made available for ongoing functionalization, transformation, and readout-is stimulating innovation across biology and medicine.

Airborne and ground based lidar measurements of the atmospheric pressure profile

NASA Technical Reports Server (NTRS)

Korb, C. Laurence; Schwemmer, Geary K.; Dombrowski, Mark; Weng, Chi Y.

1989-01-01

The first high accuracy remote measurements of the atmospheric pressure profile have been made. The measurements were made with a differential absorption lidar system that utilizes tunable alexandrite lasers. The absorption in the trough between two lines in the oxygen A-band near 760 nm was used for probing the atmosphere. Measurements of the two-dimensional structure of the pressure field were made in the troposphere from an aircraft looking down. Also, measurements of the one-dimensional structure were made from the ground looking up. Typical pressure accuracies for the aircraft measurements were 1.5-2 mbar with a 30-m vertical resolution and a 100-shot average (20 s), which corresponds to a 2-km horizontal resolution. Typical accuracies for the upward viewing ground based measurements were 2.0 mbar for a 30-m resolution and a 100-shot average.

Single-shot three-dimensional reconstruction based on structured light line pattern

NASA Astrophysics Data System (ADS)

Wang, ZhenZhou; Yang, YongMing

2018-07-01

Reconstruction of the object by single-shot is of great importance in many applications, in which the object is moving or its shape is non-rigid and changes irregularly. In this paper, we propose a single-shot structured light 3D imaging technique that calculates the phase map from the distorted line pattern. This technique makes use of the image processing techniques to segment and cluster the projected structured light line pattern from one single captured image. The coordinates of the clustered lines are extracted to form a low-resolution phase matrix which is then transformed to full-resolution phase map by spline interpolation. The 3D shape of the object is computed from the full-resolution phase map and the 2D camera coordinates. Experimental results show that the proposed method was able to reconstruct the three-dimensional shape of the object robustly from one single image.

Vulnerability of Population and Transportation Infrastructure at the East Bank of Delaware Bay Due to Coastal Flooding in Sea-Level Rise Conditions

DTIC Science & Technology

2013-04-30

resulting impact on residents and transportation infrastructure. The three-dimensional coastal ocean model FVCOM coupled with a two-dimensional...shallow water model is used to simulate hydrodynamic flooding from coastal ocean water with fine-resolution meshes, and a topography-based hydrologic... ocean model FVCOM coupled with a two-dimensional shallow water model is used to simulate hydrodynamic flooding from coastal ocean water with fine

A variable resolution nonhydrostatic global atmospheric semi-implicit semi-Lagrangian model

NASA Astrophysics Data System (ADS)

Pouliot, George Antoine

2000-10-01

The objective of this project is to develop a variable-resolution finite difference adiabatic global nonhydrostatic semi-implicit semi-Lagrangian (SISL) model based on the fully compressible nonhydrostatic atmospheric equations. To achieve this goal, a three-dimensional variable resolution dynamical core was developed and tested. The main characteristics of the dynamical core can be summarized as follows: Spherical coordinates were used in a global domain. A hydrostatic/nonhydrostatic switch was incorporated into the dynamical equations to use the fully compressible atmospheric equations. A generalized horizontal variable resolution grid was developed and incorporated into the model. For a variable resolution grid, in contrast to a uniform resolution grid, the order of accuracy of finite difference approximations is formally lost but remains close to the order of accuracy associated with the uniform resolution grid provided the grid stretching is not too significant. The SISL numerical scheme was implemented for the fully compressible set of equations. In addition, the generalized minimum residual (GMRES) method with restart and preconditioner was used to solve the three-dimensional elliptic equation derived from the discretized system of equations. The three-dimensional momentum equation was integrated in vector-form to incorporate the metric terms in the calculations of the trajectories. Using global re-analysis data for a specific test case, the model was compared to similar SISL models previously developed. Reasonable agreement between the model and the other independently developed models was obtained. The Held-Suarez test for dynamical cores was used for a long integration and the model was successfully integrated for up to 1200 days. Idealized topography was used to test the variable resolution component of the model. Nonhydrostatic effects were simulated at grid spacings of 400 meters with idealized topography and uniform flow. Using a high-resolution topographic data set and the variable resolution grid, sets of experiments with increasing resolution were performed over specific regions of interest. Using realistic initial conditions derived from re-analysis fields, nonhydrostatic effects were significant for grid spacings on the order of 0.1 degrees with orographic forcing. If the model code was adapted for use in a message passing interface (MPI) on a parallel supercomputer today, it was estimated that a global grid spacing of 0.1 degrees would be achievable for a global model. In this case, nonhydrostatic effects would be significant for most areas. A variable resolution grid in a global model provides a unified and flexible approach to many climate and numerical weather prediction problems. The ability to configure the model from very fine to very coarse resolutions allows for the simulation of atmospheric phenomena at different scales using the same code. We have developed a dynamical core illustrating the feasibility of using a variable resolution in a global model.

Three-dimensional morphological imaging of human induced pluripotent stem cells by using low-coherence quantitative phase microscopy

NASA Astrophysics Data System (ADS)

Yamauchi, Toyohiko; Kakuno, Yumi; Goto, Kentaro; Fukami, Tadashi; Sugiyama, Norikazu; Iwai, Hidenao; Mizuguchi, Yoshinori; Yamashita, Yutaka

2014-03-01

There is an increasing need for non-invasive imaging techniques in the field of stem cell research. Label-free techniques are the best choice for assessment of stem cells because the cells remain intact after imaging and can be used for further studies such as differentiation induction. To develop a high-resolution label-free imaging system, we have been working on a low-coherence quantitative phase microscope (LC-QPM). LC-QPM is a Linnik-type interference microscope equipped with nanometer-resolution optical-path-length control and capable of obtaining three-dimensional volumetric images. The lateral and vertical resolutions of our system are respectively 0.5 and 0.93 μm and this performance allows capturing sub-cellular morphological features of live cells without labeling. Utilizing LC-QPM, we reported on three-dimensional imaging of membrane fluctuations, dynamics of filopodia, and motions of intracellular organelles. In this presentation, we report three-dimensional morphological imaging of human induced pluripotent stem cells (hiPS cells). Two groups of monolayer hiPS cell cultures were prepared so that one group was cultured in a suitable culture medium that kept the cells undifferentiated, and the other group was cultured in a medium supplemented with retinoic acid, which forces the stem cells to differentiate. The volumetric images of the 2 groups show distinctive differences, especially in surface roughness. We believe that our LC-QPM system will prove useful in assessing many other stem cell conditions.

Linking models and data on vegetation structure

NASA Astrophysics Data System (ADS)

Hurtt, G. C.; Fisk, J.; Thomas, R. Q.; Dubayah, R.; Moorcroft, P. R.; Shugart, H. H.

2010-06-01

For more than a century, scientists have recognized the importance of vegetation structure in understanding forest dynamics. Now future satellite missions such as Deformation, Ecosystem Structure, and Dynamics of Ice (DESDynI) hold the potential to provide unprecedented global data on vegetation structure needed to reduce uncertainties in terrestrial carbon dynamics. Here, we briefly review the uses of data on vegetation structure in ecosystem models, develop and analyze theoretical models to quantify model-data requirements, and describe recent progress using a mechanistic modeling approach utilizing a formal scaling method and data on vegetation structure to improve model predictions. Generally, both limited sampling and coarse resolution averaging lead to model initialization error, which in turn is propagated in subsequent model prediction uncertainty and error. In cases with representative sampling, sufficient resolution, and linear dynamics, errors in initialization tend to compensate at larger spatial scales. However, with inadequate sampling, overly coarse resolution data or models, and nonlinear dynamics, errors in initialization lead to prediction error. A robust model-data framework will require both models and data on vegetation structure sufficient to resolve important environmental gradients and tree-level heterogeneity in forest structure globally.

A theoretical study on the bottlenecks of GPS phase ambiguity resolution in a CORS RTK Network

NASA Astrophysics Data System (ADS)

Odijk, D.; Teunissen, P.

2011-01-01

Crucial to the performance of GPS Network RTK positioning is that a user receives and applies correction information from a CORS Network. These corrections are necessary for the user to account for the atmospheric (ionospheric and tropospheric) delays and possibly orbit errors between his approximate location and the locations of the CORS Network stations. In order to provide the most precise corrections to users, the CORS Network processing should be based on integer resolution of the carrier phase ambiguities between the network's CORS stations. One of the main challenges is to reduce the convergence time, thus being able to quickly resolve the integer carrier phase ambiguities between the network's reference stations. Ideally, the network ambiguity resolution should be conducted within one single observation epoch, thus truly in real time. Unfortunately, single-epoch CORS Network RTK ambiguity resolution is currently not feasible and in the present contribution we study the bottlenecks preventing this. For current dual-frequency GPS the primary cause of these CORS Network integer ambiguity initialization times is the lack of a sufficiently large number of visible satellites. Although an increase in satellite number shortens the ambiguity convergence times, instantaneous CORS Network RTK ambiguity resolution is not feasible even with 14 satellites. It is further shown that increasing the number of stations within the CORS Network itself does not help ambiguity resolution much, since every new station introduces new ambiguities. The problem with CORS Network RTK ambiguity resolution is the presence of the atmospheric (mainly ionospheric) delays themselves and the fact that there are no external corrections that are sufficiently precise. We also show that external satellite clock corrections hardly contribute to CORS Network RTK ambiguity resolution, despite their quality, since the network satellite clock parameters and the ambiguities are almost completely uncorrelated. One positive is that the foreseen modernized GPS will have a very beneficial effect on CORS ambiguity resolution, because of an additional frequency with improved code precision.

Impact of comprehensive two-dimensional gas chromatography with mass spectrometry on food analysis.

PubMed

Tranchida, Peter Q; Purcaro, Giorgia; Maimone, Mariarosa; Mondello, Luigi

2016-01-01

Comprehensive two-dimensional gas chromatography with mass spectrometry has been on the separation-science scene for about 15 years. This three-dimensional method has made a great positive impact on various fields of research, and among these that related to food analysis is certainly at the forefront. The present critical review is based on the use of comprehensive two-dimensional gas chromatography with mass spectrometry in the untargeted (general qualitative profiling and fingerprinting) and targeted analysis of food volatiles; attention is focused not only on its potential in such applications, but also on how recent advances in comprehensive two-dimensional gas chromatography with mass spectrometry will potentially be important for food analysis. Additionally, emphasis is devoted to the many instances in which straightforward gas chromatography with mass spectrometry is a sufficiently-powerful analytical tool. Finally, possible future scenarios in the comprehensive two-dimensional gas chromatography with mass spectrometry food analysis field are discussed. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

DOE PAGES

Haines, Brian Michael; Grim, Gary P.; Fincke, James R.; ...

2016-07-29

Here, we present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a “CD Mixcap,” is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employmore » any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long-wavelength asymmetries degrade TT yield more than the DT yield and thus bring DT/TT neutron yield ratios into agreement with experiment. Finally, we present a detailed comparison of the flows in 2D and 3D simulations.« less

Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haines, Brian M., E-mail: bmhaines@lanl.gov; Fincke, James R.; Shah, Rahul C.

We present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a “CD Mixcap,” is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employ anymore » adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long-wavelength asymmetries degrade TT yield more than the DT yield and thus bring DT/TT neutron yield ratios into agreement with experiment. Finally, we present a detailed comparison of the flows in 2D and 3D simulations.« less

Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haines, Brian Michael; Grim, Gary P.; Fincke, James R.

Here, we present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a “CD Mixcap,” is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employmore » any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long-wavelength asymmetries degrade TT yield more than the DT yield and thus bring DT/TT neutron yield ratios into agreement with experiment. Finally, we present a detailed comparison of the flows in 2D and 3D simulations.« less

Three-dimensional dosimetry of small megavoltage radiation fields using radiochromic gels and optical CT scanning

NASA Astrophysics Data System (ADS)

Babic, Steven; McNiven, Andrea; Battista, Jerry; Jordan, Kevin

2009-04-01

The dosimetry of small fields as used in stereotactic radiotherapy, radiosurgery and intensity-modulated radiation therapy can be challenging and inaccurate due to partial volume averaging effects and possible disruption of charged particle equilibrium. Consequently, there exists a need for an integrating, tissue equivalent dosimeter with high spatial resolution to avoid perturbing the radiation beam and artificially broadening the measured beam penumbra. In this work, radiochromic ferrous xylenol-orange (FX) and leuco crystal violet (LCV) micelle gels were used to measure relative dose factors (RDFs), percent depth dose profiles and relative lateral beam profiles of 6 MV x-ray pencil beams of diameter 28.1, 9.8 and 4.9 mm. The pencil beams were produced via stereotactic collimators mounted on a Varian 2100 EX linear accelerator. The gels were read using optical computed tomography (CT). Data sets were compared quantitatively with dosimetric measurements made with radiographic (Kodak EDR2) and radiochromic (GAFChromic® EBT) film, respectively. Using a fast cone-beam optical CT scanner (Vista™), corrections for diffusion in the FX gel data yielded RDFs that were comparable to those obtained by minimally diffusing LCV gels. Considering EBT film-measured RDF data as reference, cone-beam CT-scanned LCV gel data, corrected for scattered stray light, were found to be in agreement within 0.5% and -0.6% for the 9.8 and 4.9 mm diameter fields, respectively. The validity of the scattered stray light correction was confirmed by general agreement with RDF data obtained from the same LCV gel read out with a laser CT scanner that is less prone to the acceptance of scattered stray light. Percent depth dose profiles and lateral beam profiles were found to agree within experimental error for the FX gel (corrected for diffusion), LCV gel (corrected for scattered stray light), and EBT and EDR2 films. The results from this study reveal that a three-dimensional dosimetry method utilizing optical CT-scanned radiochromic gels allows for the acquisition of a self-consistent volumetric data set in a single exposure, with sufficient spatial resolution to accurately characterize small fields.

Detailed high-resolution three-dimensional simulations of OMEGA separated reactants inertial confinement fusion experiments

NASA Astrophysics Data System (ADS)

Haines, Brian M.; Grim, Gary P.; Fincke, James R.; Shah, Rahul C.; Forrest, Chad J.; Silverstein, Kevin; Marshall, Frederic J.; Boswell, Melissa; Fowler, Malcolm M.; Gore, Robert A.; Hayes-Sterbenz, Anna C.; Jungman, Gerard; Klein, Andreas; Rundberg, Robert S.; Steinkamp, Michael J.; Wilhelmy, Jerry B.

2016-07-01

We present results from the comparison of high-resolution three-dimensional (3D) simulations with data from the implosions of inertial confinement fusion capsules with separated reactants performed on the OMEGA laser facility. Each capsule, referred to as a "CD Mixcap," is filled with tritium and has a polystyrene (CH) shell with a deuterated polystyrene (CD) layer whose burial depth is varied. In these implosions, fusion reactions between deuterium and tritium ions can occur only in the presence of atomic mix between the gas fill and shell material. The simulations feature accurate models for all known experimental asymmetries and do not employ any adjustable parameters to improve agreement with experimental data. Simulations are performed with the RAGE radiation-hydrodynamics code using an Implicit Large Eddy Simulation (ILES) strategy for the hydrodynamics. We obtain good agreement with the experimental data, including the DT/TT neutron yield ratios used to diagnose mix, for all burial depths of the deuterated shell layer. Additionally, simulations demonstrate good agreement with converged simulations employing explicit models for plasma diffusion and viscosity, suggesting that the implicit sub-grid model used in ILES is sufficient to model these processes in these experiments. In our simulations, mixing is driven by short-wavelength asymmetries and longer-wavelength features are responsible for developing flows that transport mixed material towards the center of the hot spot. Mix material transported by this process is responsible for most of the mix (DT) yield even for the capsule with a CD layer adjacent to the tritium fuel. Consistent with our previous results, mix does not play a significant role in TT neutron yield degradation; instead, this is dominated by the displacement of fuel from the center of the implosion due to the development of turbulent instabilities seeded by long-wavelength asymmetries. Through these processes, the long-wavelength asymmetries degrade TT yield more than the DT yield and thus bring DT/TT neutron yield ratios into agreement with experiment. Finally, we present a detailed comparison of the flows in 2D and 3D simulations.

Diffusion-prepared stimulated-echo turbo spin echo (DPsti-TSE): An eddy current-insensitive sequence for three-dimensional high-resolution and undistorted diffusion-weighted imaging.

PubMed

Zhang, Qinwei; Coolen, Bram F; Versluis, Maarten J; Strijkers, Gustav J; Nederveen, Aart J

2017-07-01

In this study, we present a new three-dimensional (3D), diffusion-prepared turbo spin echo sequence based on a stimulated-echo read-out (DPsti-TSE) enabling high-resolution and undistorted diffusion-weighted imaging (DWI). A dephasing gradient in the diffusion preparation module and rephasing gradients in the turbo spin echo module create stimulated echoes, which prevent signal loss caused by eddy currents. Near to perfect agreement of apparent diffusion coefficient (ADC) values between DPsti-TSE and diffusion-weighted echo planar imaging (DW-EPI) was demonstrated in both phantom transient signal experiments and phantom imaging experiments. High-resolution and undistorted DPsti-TSE was demonstrated in vivo in prostate and carotid vessel wall. 3D whole-prostate DWI was achieved with four b values in only 6 min. Undistorted ADC maps of the prostate peripheral zone were obtained at low and high imaging resolutions with no change in mean ADC values [(1.60 ± 0.10) × 10 -3 versus (1.60 ± 0.02) × 10 -3  mm 2 /s]. High-resolution 3D DWI of the carotid vessel wall was achieved in 12 min, with consistent ADC values [(1.40 ± 0.23) × 10 -3  mm 2 /s] across different subjects, as well as slice locations through the imaging volume. This study shows that DPsti-TSE can serve as a robust 3D diffusion-weighted sequence and is an attractive alternative to the traditional two-dimensional DW-EPI approaches. Copyright © 2017 John Wiley & Sons, Ltd.

High-Resolution Three-Dimensional Computed Tomography for Assessing Complications Related to Intrathecal Drug Delivery.

PubMed

Morgalla, Matthias; Fortunato, Marcos; Azam, Ala; Tatagiba, Marcos; Lepski, Guillherme

2016-07-01

The assessment of the functionality of intrathecal drug delivery (IDD) systems remains difficult and time-consuming. Catheter-related problems are still very common, and sometimes difficult to diagnose. The aim of the present study is to investigate the accuracy of high-resolution three-dimensional computed tomography (CT) in order to detect catheter-related pump dysfunction. An observational, retrospective investigation. Academic medical center in Germany. We used high-resolution three dimensional (3D) computed tomography with volume rendering technique (VRT) or fluoroscopy and conventional axial-CT to assess IDD-related complications in 51 patients from our institution who had IDD systems implanted for the treatment of chronic pain or spasticity. Twelve patients (23.5%) presented a total of 22 complications. The main type of complication in our series was catheter-related (50%), followed by pump failure, infection, and inappropriate refilling. Fluoroscopy and conventional CT were used in 12 cases. High-resolution 3D CT VRT scan was used in 35 instances with suspected yet unclear complications. Using 3D-CT (VRT) the sensitivity was 58.93% - 100% (CI 95%) and the specificity 87.54% - 100% (CI 95%).The positive predictive value was 58.93% - 100% (CI 95%) and the negative predictive value: 87.54% - 100% (CI 95%).Fluoroscopy and axial CT as a combined diagnostic tool had a sensitivity of 8.3% - 91.7% (CI 95%) and a specificity of 62.9% - 100% (CI 95%). The positive predictive value was 19.29% - 100% (CI 95%) and the negative predictive value: 44.43% - 96.89% (CI 95%). This study is limited by its observational design and the small number of cases. High-resolution 3D CT VRT is a non- invasive method that can identify IDD-related complications with more precision than axial CT and fluoroscopy.

Three-Dimensional Imaging and Quantification of Biomass and Biofilms in Porous Media

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dorthe Wildenschild

2012-10-10

A new method to resolve biofilms in three dimensions in porous media using high-resolution synchrotron-based x-ray computed microtomography (CMT) has been developed. Imaging biofilms in porous media without disturbing the natural spatial arrangement of the porous media and associated biofilm has been a challenging task, primarily because porous media generally precludes conventional imaging via optical microscopy; x-ray tomography offers a potential alternative. One challenge for using this method is that most conventional x-ray contrast agents are water-soluble and easily diffuse into biofilms. To overcome this problem, silver-coated microspheres were added to the fluid phase to create an x-ray contrast thatmore » does not diffuse into the biofilm mass. Using this approach, biofilm imaging in porous media was accomplished with sufficient contrast to differentiate between the biomass- and fluid-filled pore spaces. The method was validated by using a two-dimensional micro-model flow cell where both light microscopy and CMT imaging were used to im age the biofilm. The results of this work has been published in Water Resources Research (Iltis et al., 2010). Additional work needs to be done to optimize this imaging approach, specifically, we find that the quality of the images are highly dependent on the coverage of the biofilm with Ag particles, - which means that we may have issues in dead-end pore space and for very low density (fluffy) biofilms. What we can image for certain with this technique is the biofilm surface that is well-connected to flow paths and thus well-supplied with nutrients etc.« less

Two-dimensional gas chromatographic analysis of ambient light hydrocarbons.

PubMed

Liao, Wei-Chen; Ou-Yang, Cheng-Feng; Wang, Chieh-Heng; Chang, Chih-Chung; Wang, Jia-Lin

2013-06-14

Ambient level hydrocarbons lighter than C6 were analyzed by the Deans switch-modulated comprehensive two-dimensional gas chromatography (GC×GC) method with flame ionization detection (FID). A thermal desorption (TD) device built in-house connects the GC×GC system to pre-concentrate the target compounds at ambient levels prior to GC analysis. Because the conventional orthogonality based on polarity difference for normal GC×GC separation does not provide sufficient retention for the target compounds of extremely high volatility, the orthogonality of non-polar vs. adsorptive force was adopted instead. The system employed a 100% polydimethyl siloxane column serving as the first-dimension column to provide separation based on dispersive interaction, with a short PLOT column serving as the second-dimension column to provide the needed retention based on gas-solid adsorption interactions. The shortest possible length of the PLOT column was tested to minimize the modulation period (PM) and wraparound and, at the same time, to maintain the desired resolution. The tests led to the final optimal parameters of 1.1m for the PLOT column length, 9s for the PM, 0.013 for the modulation duty cycle (DC) and a modulation ratio (MR) of 3.7 with minimal wraparound. Important criteria for quality assurance of precision and linearity are reported. The low cost and ease of construction and operation make the in-house Deans switch TD-GC×GC-FID system practical and useful for the analysis of light hydrocarbons in urban or industrial environments. Copyright © 2013 Elsevier B.V. All rights reserved.

The 3-dimensional grid: a novel approach to stereoelectroencephalography.

PubMed

Munyon, Charles; Sweet, Jennifer; Luders, Hans; Lhatoo, Samden; Miller, Jonathan

2015-03-01

Successful surgical treatment of epilepsy requires accurate definition of areas of ictal onset and eloquent brain. Although invasive monitoring can help, subdural grids cannot sample sulci or subcortical tissue; traditional stereoelectroencephalography depth electrodes are usually placed too far apart to provide sufficient resolution for mapping. To report a strategy of depth electrode placement in a dense array to allow precise anatomic localization of epileptic and eloquent cortex. Twenty patients with medically intractable epilepsy either poorly localized or found to arise adjacent to eloquent areas underwent placement of arrays of depth electrodes into and around the putative area of seizure onset with the use of framed stereotaxy. Each array consisted of a "grid" of parallel electrodes in a rectangular pattern with 1 cm between entry sites. In a subset of patients, a few electrodes were placed initially, with additional electrodes placed in a second stage. Trajectories were modified to avoid cortical vessels defined on magnetic resonance imaging. Patients were monitored for 4 to 21 days to establish the precise location of seizure onset. Stimulation was performed to map cortical and subcortical eloquent regions. Electrode locations were coregistered for frameless stereotaxy during subsequent resection of seizure focus. Two hundred fifty-four electrodes were implanted. Discrete regions of seizure onset and functional cortex were identified, which were used during resection to remove epileptogenic tissue while preserving eloquent areas. There were no hemorrhagic or infectious complications; no patient suffered permanent neurological deficit. The 3-dimensional intraparenchymal grid is useful for identifying the location and extent of epileptic and eloquent brain.

High-resolution three-dimensional magnetic resonance imaging of mouse lung in situ.

PubMed

Scadeng, Miriam; Rossiter, Harry B; Dubowitz, David J; Breen, Ellen C

2007-01-01

This study establishes a method for high-resolution isotropic magnetic resonance (MR) imaging of mouse lungs using tracheal liquid-instillation to remove MR susceptibility artifacts. C57BL/6J mice were instilled sequentially with perfluorocarbon and phosphate-buffered saline to an airway pressure of 10, 20, or 30 cm H2O. Imaging was performed in a 7T MR scanner using a 2.5-cm Quadrature volume coil and a 3-dimensional (3D) FLASH imaging sequence. Liquid-instillation removed magnetic susceptibility artifacts and allowed lung structure to be viewed at an isotropic resolution of 78-90 microm. Instilled liquid and modeled lung volumes were well correlated (R = 0.92; P < 0.05) and differed by a constant tissue volume (220 +/- 92 microL). 3D image renderings allowed differences in structural dimensions (volumes and areas) to be accurately measured at each inflation pressure. These data demonstrate the efficacy of pulmonary liquid instillation for in situ high-resolution MR imaging of mouse lungs for accurate measurement of pulmonary airway, parenchymal, and vascular structures.

Using high-resolution displays for high-resolution cardiac data.

PubMed

Goodyer, Christopher; Hodrien, John; Wood, Jason; Kohl, Peter; Brodlie, Ken

2009-07-13

The ability to perform fast, accurate, high-resolution visualization is fundamental to improving our understanding of anatomical data. As the volumes of data increase from improvements in scanning technology, the methods applied to visualization must evolve. In this paper, we address the interactive display of data from high-resolution magnetic resonance imaging scanning of a rabbit heart and subsequent histological imaging. We describe a visualization environment involving a tiled liquid crystal display panel display wall and associated software, which provides an interactive and intuitive user interface. The oView software is an OpenGL application that is written for the VR Juggler environment. This environment abstracts displays and devices away from the application itself, aiding portability between different systems, from desktop PCs to multi-tiled display walls. Portability between display walls has been demonstrated through its use on walls at the universities of both Leeds and Oxford. We discuss important factors to be considered for interactive two-dimensional display of large three-dimensional datasets, including the use of intuitive input devices and level of detail aspects.

In vivo, high-resolution, three-dimensional imaging of port wine stain microvasculature in human skin.

PubMed

Liu, Gangjun; Jia, Wangcun; Nelson, J Stuart; Chen, Zhongping

2013-12-01

Port-wine stain (PWS) is a congenital, progressive vascular malformation of the dermis. The use of optical coherence tomography (OCT) for the characterization of blood vessels in PWS skin has been demonstrated by several groups. In the past few years, advances in OCT technology have greatly increased imaging speed. Sophisticated numerical algorithms have improved the sensitivity of Doppler OCT dramatically. These improvements have enabled the noninvasive, high-resolution, three-dimensional functional imaging of PWS skin. Here, we demonstrate high-resolution, three-dimensional, microvasculature imaging of PWS and normal skin using Doppler OCT technique. The OCT system uses a swept source laser which has a central wavelength of 1,310 nm, an A-line rate of 50 kHz and a total average power of 16 mW. The system uses a handheld imaging probe and has an axial resolution of 9.3 µm in air and a lateral resolution of approximately 15 µm. Images were acquired from PWS subjects at the Beckman Laser Institute and Medical Clinic. Microvasculature of the PWS skin and normal skin were obtained from the PWS subject. High-resolution, three-dimensional microvasculature of PWS and normal skin were obtained. Many enlarged PWS vessels are detected in the dermis down to 1.0 mm below the PWS skin surface. In one subject, the blood vessel diameters range from 40 to 90 µm at the epidermal-dermal junction and increase up to 300-500 µm at deeper regions 700-1,000 µm below skin surface. The blood vessels close to the epidermal-dermal junction are more uniform, in terms of diameter. The more tortuous and dilated PWS blood vessels are located at deeper regions 600-1,000 µm below the skin surface. In another subject example, the PWS skin blood vessels are dilated at very superficial layers at a depth less than 500 µm below the skin surface. The PWS skin vessel diameters range from 60 to 650 µm, with most vessels having a diameter of around 200 µm. OCT can be used to quantitatively image in vivo skin micro-vasculature. Analysis of the PWS and normal skin blood vessels were performed and the results can provide quantitative information to optimize laser treatment on an individual patient basis. © 2013 Wiley Periodicals, Inc.

Selective separation of fluorinated compounds from complex organic mixtures by pyrolysis-comprehensive two-dimensional gas chromatography coupled to high-resolution time-of-flight mass spectrometry.

PubMed

Nakajima, Yoji; Arinami, Yuko; Yamamoto, Kiyoshi

2014-12-29

The usefulness of comprehensive two-dimensional gas chromatography (GC×GC) was demonstrated for the selective separation of fluorinated compounds from organic mixtures, such as kerosene/perfluorokerosene mixtures, pyrolysis products derived from polyethylene/ethylene-tetrafluoroethylene alternating copolymer mixture and poly[2-(perfluorohexyl)ethyl acrylate]. Perfluorocarbons were completely separated from hydrocarbons in the two-dimensional chromatogram. Fluorohydrocarbons in the pyrolysis products of polyethylene/ethylene-tetrafluoroethylene alternating copolymer mixture were selectively isolated from their hydrocarbon counterparts and regularly arranged according to their chain length and fluorine content in the two-dimensional chromatogram. A reliable structural analysis of the fluorohydrocarbons was achieved by combining effective GC×GC positional information with accurate mass spectral data obtained by high-resolution time-of-flight mass spectrometry (HRTOF-MS). 2-(Perfluorohexyl)ethyl acrylate monomer, dimer, and trimer as well as 2-(perfluorohexyl)ethyl alcohol in poly[2-(perfluorohexyl)ethyl acrylate] pyrolysis products were detected in the bottommost part of the two-dimensional chromatogram with separation from hydrocarbons possessing terminal structure information about the polymer, such as α-methylstyrene. Pyrolysis-GC×GC/HRTOF-MS appeared particularly suitable for the characterization of fluorinated polymer microstructures, such as monomer sequences and terminal groups. Copyright © 2014 Elsevier B.V. All rights reserved.

Three-Dimensional Anisotropic Acoustic and Elastic Full-Waveform Seismic Inversion

NASA Astrophysics Data System (ADS)

Warner, M.; Morgan, J. V.

2013-12-01

Three-dimensional full-waveform inversion is a high-resolution, high-fidelity, quantitative, seismic imaging technique that has advanced rapidly within the oil and gas industry. The method involves the iterative improvement of a starting model using a series of local linearized updates to solve the full non-linear inversion problem. During the inversion, forward modeling employs the full two-way three-dimensional heterogeneous anisotropic acoustic or elastic wave equation to predict the observed raw field data, wiggle-for-wiggle, trace-by-trace. The method is computationally demanding; it is highly parallelized, and runs on large multi-core multi-node clusters. Here, we demonstrate what can be achieved by applying this newly practical technique to several high-density 3D seismic datasets that were acquired to image four contrasting sedimentary targets: a gas cloud above an oil reservoir, a radially faulted dome, buried fluvial channels, and collapse structures overlying an evaporate sequence. We show that the resulting anisotropic p-wave velocity models match in situ measurements in deep boreholes, reproduce detailed structure observed independently on high-resolution seismic reflection sections, accurately predict the raw seismic data, simplify and sharpen reverse-time-migrated reflection images of deeper horizons, and flatten Kirchhoff-migrated common-image gathers. We also show that full-elastic 3D full-waveform inversion of pure pressure data can generate a reasonable shear-wave velocity model for one of these datasets. For two of the four datasets, the inclusion of significant transversely isotropic anisotropy with a vertical axis of symmetry was necessary in order to fit the kinematics of the field data properly. For the faulted dome, the full-waveform-inversion p-wave velocity model recovers the detailed structure of every fault that can be seen on coincident seismic reflection data. Some of the individual faults represent high-velocity zones, some represent low-velocity zones, some have more-complex internal structure, and some are visible merely as offsets between two regions with contrasting velocity. Although this has not yet been demonstrated quantitatively for this dataset, it seems likely that at least some of this fine structure in the recovered velocity model is related to the detailed lithology, strain history and fluid properties within the individual faults. We have here applied this technique to seismic data that were acquired by the extractive industries, however this inversion scheme is immediately scalable and applicable to a much wider range of problems given sufficient quality and density of observed data. Potential targets range from shallow magma chambers beneath active volcanoes, through whole-crustal sections across plate boundaries, to regional and whole-Earth models.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jimenez, Bienvenido; Novo, Vicente

We provide second-order necessary and sufficient conditions for a point to be an efficient element of a set with respect to a cone in a normed space, so that there is only a small gap between necessary and sufficient conditions. To this aim, we use the common second-order tangent set and the asymptotic second-order cone utilized by Penot. As an application we establish second-order necessary conditions for a point to be a solution of a vector optimization problem with an arbitrary feasible set and a twice Frechet differentiable objective function between two normed spaces. We also establish second-order sufficient conditionsmore » when the initial space is finite-dimensional so that there is no gap with necessary conditions. Lagrange multiplier rules are also given.« less

Three-Dimensional Media Technologies: Potentials for Study in Visual Literacy.

ERIC Educational Resources Information Center

Thwaites, Hal

This paper presents an overview of three-dimensional media technologies (3Dmt). Many of the new 3Dmt are the direct result of interactions of computing, communications, and imaging technologies. Computer graphics are particularly well suited to the creation of 3D images due to the high resolution and programmable nature of the current displays.…

Semidirect computation of three-dimensional viscous flows over suction holes in laminar flow control surfaces

NASA Technical Reports Server (NTRS)

Roache, P. J.

1979-01-01

A summary is given of the attempts made to apply semidirect methods to the calculation of three-dimensional viscous flows over suction holes in laminar flow control surfaces. The attempts were all unsuccessful, due to either (1) lack of resolution capability, (2) lack of computer efficiency, or (3) instability.

Interior volume of (1 + D)-dimensional Schwarzschild black hole

NASA Astrophysics Data System (ADS)

Bhaumik, Nilanjandev; Majhi, Bibhas Ranjan

2018-01-01

We calculate the maximum interior volume, enclosed by the event horizon, of a (1 + D)-dimensional Schwarzschild black hole. Taking into account the mass change due to Hawking radiation, we show that the volume increases towards the end of the evaporation. This fact is not new as it has been observed earlier for four-dimensional case. The interesting point we observe is that this increase rate decreases towards the higher value of space dimensions D; i.e. it is a decelerated expansion of volume with the increase of spatial dimensions. This implies that for a sufficiently large D, the maximum interior volume does not change. The possible implications of these results are also discussed.

Superresolution parallel magnetic resonance imaging: Application to functional and spectroscopic imaging

PubMed Central

Otazo, Ricardo; Lin, Fa-Hsuan; Wiggins, Graham; Jordan, Ramiro; Sodickson, Daniel; Posse, Stefan

2009-01-01

Standard parallel magnetic resonance imaging (MRI) techniques suffer from residual aliasing artifacts when the coil sensitivities vary within the image voxel. In this work, a parallel MRI approach known as Superresolution SENSE (SURE-SENSE) is presented in which acceleration is performed by acquiring only the central region of k-space instead of increasing the sampling distance over the complete k-space matrix and reconstruction is explicitly based on intra-voxel coil sensitivity variation. In SURE-SENSE, parallel MRI reconstruction is formulated as a superresolution imaging problem where a collection of low resolution images acquired with multiple receiver coils are combined into a single image with higher spatial resolution using coil sensitivities acquired with high spatial resolution. The effective acceleration of conventional gradient encoding is given by the gain in spatial resolution, which is dictated by the degree of variation of the different coil sensitivity profiles within the low resolution image voxel. Since SURE-SENSE is an ill-posed inverse problem, Tikhonov regularization is employed to control noise amplification. Unlike standard SENSE, for which acceleration is constrained to the phase-encoding dimension/s, SURE-SENSE allows acceleration along all encoding directions — for example, two-dimensional acceleration of a 2D echo-planar acquisition. SURE-SENSE is particularly suitable for low spatial resolution imaging modalities such as spectroscopic imaging and functional imaging with high temporal resolution. Application to echo-planar functional and spectroscopic imaging in human brain is presented using two-dimensional acceleration with a 32-channel receiver coil. PMID:19341804

High-Resolution Isotropic Three-Dimensional MR Imaging of the Extraforaminal Segments of the Cranial Nerves.

PubMed

Wen, Jessica; Desai, Naman S; Jeffery, Dean; Aygun, Nafi; Blitz, Ari

2018-02-01

High-resolution isotropic 3-dimensional (D) MR imaging with and without contrast is now routinely used for imaging evaluation of cranial nerve anatomy and pathologic conditions. The anatomic details of the extraforaminal segments are well-visualized on these techniques. A wide range of pathologic entities may cause enhancement or displacement of the nerve, which is now visible to an extent not available on standard 2D imaging. This article highlights the anatomy of extraforaminal segments of the cranial nerves and uses select cases to illustrate the utility and power of these sequences, with a focus on constructive interference in steady-state. Copyright © 2017 Elsevier Inc. All rights reserved.

Compton imaging tomography technique for NDE of large nonuniform structures

NASA Astrophysics Data System (ADS)

Grubsky, Victor; Romanov, Volodymyr; Patton, Ned; Jannson, Tomasz

2011-09-01

In this paper we describe a new nondestructive evaluation (NDE) technique called Compton Imaging Tomography (CIT) for reconstructing the complete three-dimensional internal structure of an object, based on the registration of multiple two-dimensional Compton-scattered x-ray images of the object. CIT provides high resolution and sensitivity with virtually any material, including lightweight structures and organics, which normally pose problems in conventional x-ray computed tomography because of low contrast. The CIT technique requires only one-sided access to the object, has no limitation on the object's size, and can be applied to high-resolution real-time in situ NDE of large aircraft/spacecraft structures and components. Theoretical and experimental results will be presented.

Computational cell quantification in the human brain tissues based on hard x-ray phase-contrast tomograms

NASA Astrophysics Data System (ADS)

Hieber, Simone E.; Bikis, Christos; Khimchenko, Anna; Schulz, Georg; Deyhle, Hans; Thalmann, Peter; Chicherova, Natalia; Rack, Alexander; Zdora, Marie-Christine; Zanette, Irene; Schweighauser, Gabriel; Hench, Jürgen; Müller, Bert

2016-10-01

Cell visualization and counting plays a crucial role in biological and medical research including the study of neurodegenerative diseases. The neuronal cell loss is typically determined to measure the extent of the disease. Its characterization is challenging because the cell density and size already differs by more than three orders of magnitude in a healthy cerebellum. Cell visualization is commonly performed by histology and fluorescence microscopy. These techniques are limited to resolve complex microstructures in the third dimension. Phase- contrast tomography has been proven to provide sufficient contrast in the three-dimensional imaging of soft tissue down to the cell level and, therefore, offers the basis for the three-dimensional segmentation. Within this context, a human cerebellum sample was embedded in paraffin and measured in local phase-contrast mode at the beamline ID19 (ESRF, Grenoble, France) and the Diamond Manchester Imaging Branchline I13-2 (Diamond Light Source, Didcot, UK). After the application of Frangi-based filtering the data showed sufficient contrast to automatically identify the Purkinje cells and to quantify their density to 177 cells per mm3 within the volume of interest. Moreover, brain layers were segmented in a region of interest based on edge detection. Subsequently performed histological analysis validated the presence of the cells, which required a mapping from the two- dimensional histological slices to the three-dimensional tomogram. The methodology can also be applied to further tissue types and shows potential for the computational tissue analysis in health and disease.

GRAIL at Mercury: Coherent Laser Tracking for Geophysics

NASA Astrophysics Data System (ADS)

Mazarico, E.; Goossens, S.; Genova, A.; Sun, X.; Yang, G.

2018-05-01

We present an instrument concept for satellite-to-satellite tracking at optical wavelength to measure the gravity field of Mercury with sufficient accuracy and resolution to significantly advance our understanding of its geophysical evolution.

In vivo spectral micro-imaging of tissue

DOEpatents

Demos, Stavros G; Urayama, Shiro; Lin, Bevin; Saroufeem, Ramez; Ghobrial, Moussa

2012-11-27

In vivo endoscopic methods an apparatuses for implementation of fluorescence and autofluorescence microscopy, with and without the use of exogenous agents, effectively (with resolution sufficient to image nuclei) visualize and categorize various abnormal tissue forms.

Development of MMC Gamma Detectors for Nuclear Analysis

NASA Astrophysics Data System (ADS)

Bates, C. R.; Pies, C.; Kempf, S.; Gastaldo, L.; Fleischmann, A.; Enss, C.; Friedrich, S.

2014-09-01

Non-destructive assay (NDA) of nuclear materials would benefit from gamma detectors with improved energy resolution in cases where line overlap in current Ge detectors limits NDA accuracy. We are developing metallic magnetic calorimeter gamma-detectors for this purpose by electroplating 150 m thick Au absorbers into microfabricated molds on top of Au:Er sensors. Initial tests under non-optimized conditions show an energy resolution of 200 eV FWHM at 60 keV. Monte Carlo simulations illustrate that this resolution is starting to be sufficient for direct detection of Pu in plutonium separated from spent nuclear fuel.

Volumetric optoacoustic monitoring of endovenous laser treatments

NASA Astrophysics Data System (ADS)

Fehm, Thomas F.; Deán-Ben, Xosé L.; Schaur, Peter; Sroka, Ronald; Razansky, Daniel

2016-03-01

Chronic venous insufficiency (CVI) is one of the most common medical conditions with reported prevalence estimates as high as 30% in the adult population. Although conservative management with compression therapy may improve the symptoms associated with CVI, healing often demands invasive procedures. Besides established surgical methods like vein stripping or bypassing, endovenous laser therapy (ELT) emerged as a promising novel treatment option during the last 15 years offering multiple advantages such as less pain and faster recovery. Much of the treatment success hereby depends on monitoring of the treatment progression using clinical imaging modalities such as Doppler ultrasound. The latter however do not provide sufficient contrast, spatial resolution and three-dimensional imaging capacity which is necessary for accurate online lesion assessment during treatment. As a consequence, incidence of recanalization, lack of vessel occlusion and collateral damage remains highly variable among patients. In this study, we examined the capacity of volumetric optoacoustic tomography (VOT) for real-time monitoring of ELT using an ex-vivo ox foot model. ELT was performed on subcutaneous veins while optoacoustic signals were acquired and reconstructed in real-time and at a spatial resolution in the order of 200μm. VOT images showed spatio-temporal maps of the lesion progression, characteristics of the vessel wall, and position of the ablation fiber's tip during the pull back. It was also possible to correlate the images with the temperature elevation measured in the area adjacent to the ablation spot. We conclude that VOT is a promising tool for providing online feedback during endovenous laser therapy.

Wide-field computational imaging of pathology slides using lens-free on-chip microscopy.

PubMed

Greenbaum, Alon; Zhang, Yibo; Feizi, Alborz; Chung, Ping-Luen; Luo, Wei; Kandukuri, Shivani R; Ozcan, Aydogan

2014-12-17

Optical examination of microscale features in pathology slides is one of the gold standards to diagnose disease. However, the use of conventional light microscopes is partially limited owing to their relatively high cost, bulkiness of lens-based optics, small field of view (FOV), and requirements for lateral scanning and three-dimensional (3D) focus adjustment. We illustrate the performance of a computational lens-free, holographic on-chip microscope that uses the transport-of-intensity equation, multi-height iterative phase retrieval, and rotational field transformations to perform wide-FOV imaging of pathology samples with comparable image quality to a traditional transmission lens-based microscope. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for mechanical focus adjustment and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. Using this lens-free on-chip microscope, we successfully imaged invasive carcinoma cells within human breast sections, Papanicolaou smears revealing a high-grade squamous intraepithelial lesion, and sickle cell anemia blood smears over a FOV of 20.5 mm(2). The resulting wide-field lens-free images had sufficient image resolution and contrast for clinical evaluation, as demonstrated by a pathologist's blinded diagnosis of breast cancer tissue samples, achieving an overall accuracy of ~99%. By providing high-resolution images of large-area pathology samples with 3D digital focus adjustment, lens-free on-chip microscopy can be useful in resource-limited and point-of-care settings. Copyright © 2014, American Association for the Advancement of Science.

Validation of a 30 m resolution flood hazard model of the conterminous United States

NASA Astrophysics Data System (ADS)

Wing, Oliver E. J.; Bates, Paul D.; Sampson, Christopher C.; Smith, Andrew M.; Johnson, Kris A.; Erickson, Tyler A.

2017-09-01

This paper reports the development of a ˜30 m resolution two-dimensional hydrodynamic model of the conterminous U.S. using only publicly available data. The model employs a highly efficient numerical solution of the local inertial form of the shallow water equations which simulates fluvial flooding in catchments down to 50 km2 and pluvial flooding in all catchments. Importantly, we use the U.S. Geological Survey (USGS) National Elevation Dataset to determine topography; the U.S. Army Corps of Engineers National Levee Dataset to explicitly represent known flood defenses; and global regionalized flood frequency analysis to characterize return period flows and rainfalls. We validate these simulations against the complete catalogue of Federal Emergency Management Agency (FEMA) Special Flood Hazard Area (SFHA) maps and detailed local hydraulic models developed by the USGS. Where the FEMA SFHAs are based on high-quality local models, the continental-scale model attains a hit rate of 86%. This correspondence improves in temperate areas and for basins above 400 km2. Against the higher quality USGS data, the average hit rate reaches 92% for the 1 in 100 year flood, and 90% for all flood return periods. Given typical hydraulic modeling uncertainties in the FEMA maps and USGS model outputs (e.g., errors in estimating return period flows), it is probable that the continental-scale model can replicate both to within error. The results show that continental-scale models may now offer sufficient rigor to inform some decision-making needs with dramatically lower cost and greater coverage than approaches based on a patchwork of local studies.

Development of scanning holographic display using MEMS SLM

NASA Astrophysics Data System (ADS)

Takaki, Yasuhiro

2016-10-01

Holography is an ideal three-dimensional (3D) display technique, because it produces 3D images that naturally satisfy human 3D perception including physiological and psychological factors. However, its electronic implementation is quite challenging because ultra-high resolution is required for display devices to provide sufficient screen size and viewing zone. We have developed holographic display techniques to enlarge the screen size and the viewing zone by use of microelectromechanical systems spatial light modulators (MEMS-SLMs). Because MEMS-SLMs can generate hologram patterns at a high frame rate, the time-multiplexing technique is utilized to virtually increase the resolution. Three kinds of scanning systems have been combined with MEMS-SLMs; the screen scanning system, the viewing-zone scanning system, and the 360-degree scanning system. The screen scanning system reduces the hologram size to enlarge the viewing zone and the reduced hologram patterns are scanned on the screen to increase the screen size: the color display system with a screen size of 6.2 in. and a viewing zone angle of 11° was demonstrated. The viewing-zone scanning system increases the screen size and the reduced viewing zone is scanned to enlarge the viewing zone: a screen size of 2.0 in. and a viewing zone angle of 40° were achieved. The two-channel system increased the screen size to 7.4 in. The 360-degree scanning increases the screen size and the reduced viewing zone is scanned circularly: the display system having a flat screen with a diameter of 100 mm was demonstrated, which generates 3D images viewed from any direction around the flat screen.

Collocated ionosonde and dense GPS/GLONASS network measurements of midlatitude MSTIDs

NASA Astrophysics Data System (ADS)

Sherstyukov, R. O.; Akchurin, A. D.; Sherstyukov, O. N.

2018-04-01

To analyze midlatitude medium-scale travelling ionospheric disturbances (MSTIDs) over Kazan (55.5°N, 49°E), Russia, the sufficiently dense network of GNSS receivers (more than 150 ground-based stations) were used. For the first time, daytime MSTIDs in the form of their main signature (band structure) on high-resolution two-dimensional maps of the total electron content perturbation (TEC maps) are compared with ionosonde data with a high temporal resolution. For a pair of events, a relationship between southwestward TEC perturbations and evolution of F2 layer traces was established. So F2 peak frequency varied in antiphase to TEC perturbations. The ionograms show that during the movement of plasma depletion band (overhead ionosonde) the F2 peak frequency is the highest, and vice versa, for the plasma enhancement band, the F2 peak frequency is the lowest. One possible explanation may be a greater inclination of the radio beam from the vertical during the placement of a plasma enhancement band above the ionosonde, as evidenced by the absence of multiple reflections and the increased occurrence rate of additional cusp trace. Another possible explanation may be the redistribution of the electron content in the topside ionosphere with a small decrease in the F peak concentration of the layer with a small increase in TEC along the line-of-sight. Analysis of F2 peak frequency variation shows that observed peak-to-peak values of TEC perturbation equal to 0.4 and 1 TECU correspond to the values of ΔN/N equal to 13% and 28%. The need for further research is evident.

Creation of parallel algorithms for the solution of problems of gas dynamics on multi-core computers and GPU

NASA Astrophysics Data System (ADS)

Rybakin, B.; Bogatencov, P.; Secrieru, G.; Iliuha, N.

2013-10-01

The paper deals with a parallel algorithm for calculations on multiprocessor computers and GPU accelerators. The calculations of shock waves interaction with low-density bubble results and the problem of the gas flow with the forces of gravity are presented. This algorithm combines a possibility to capture a high resolution of shock waves, the second-order accuracy for TVD schemes, and a possibility to observe a low-level diffusion of the advection scheme. Many complex problems of continuum mechanics are numerically solved on structured or unstructured grids. To improve the accuracy of the calculations is necessary to choose a sufficiently small grid (with a small cell size). This leads to the drawback of a substantial increase of computation time. Therefore, for the calculations of complex problems it is reasonable to use the method of Adaptive Mesh Refinement. That is, the grid refinement is performed only in the areas of interest of the structure, where, e.g., the shock waves are generated, or a complex geometry or other such features exist. Thus, the computing time is greatly reduced. In addition, the execution of the application on the resulting sequence of nested, decreasing nets can be parallelized. Proposed algorithm is based on the AMR method. Utilization of AMR method can significantly improve the resolution of the difference grid in areas of high interest, and from other side to accelerate the processes of the multi-dimensional problems calculating. Parallel algorithms of the analyzed difference models realized for the purpose of calculations on graphic processors using the CUDA technology [1].

Detail Extraction from Electron Backscatter Diffraction Patterns

NASA Astrophysics Data System (ADS)

Basinger, Jay

Cross-correlation based analysis of electron backscatter diffraction (EBSD) patterns and the use of simulated reference patterns has opened up entirely new avenues of insight into local lattice properties within EBSD scans. The benefits of accessing new levels of orientation resolution and multiple types of previously inaccessible data measures are accompanied with new challenges in characterizing microscope geometry and other error previously ignored in EBSD systems. The foremost of these challenges, when using simulated patterns in high resolution EBSD (HR-EBSD), is the determination of pattern center (the location on the sample from which the EBSD pattern originated) with sufficient accuracy to avoid the introduction of phantom lattice rotations and elastic strain into these highly sensitive measures. This dissertation demonstrates how to greatly improve pattern center determination. It also presents a method for the extraction of grain boundary plane information from single two-dimensional surface scans. These are accomplished through the use of previously un-accessed detail within EBSD images, coupled with physical models of the backscattering phenomena. A software algorithm is detailed and applied for the determination of pattern center with an accuracy of ˜0.03% of the phosphor screen width, or ˜10μm. This resolution makes it possible to apply a simulated pattern method (developed at BYU) in HR-EBSD, with several important benefits over the original HR-EBSD approach developed by Angus Wilkinson. Experimental work is done on epitaxially-grown silicon and germanium in order to gauge the precision of HR-EBSD with simulated reference patterns using the new pattern center calibration approach. It is found that strain resolution with a calibrated pattern center and simulated reference patterns can be as low as 7x10-4. Finally, Monte Carlo-based models of the electron interaction volume are used in conjunction with pattern-mixing-strength curves of line scans crossing grain boundaries in order to recover 3D grain boundary plane information. Validation of the approach is done using 3D serial scan data and coherent twin boundaries in tantalum and copper. The proposed method for recovery of grain boundary plane orientation exhibits an average error of 3 degrees.

Studying the Surfaces of the Icy Galilean Satellites With JIMO

NASA Astrophysics Data System (ADS)

Prockter, L.; Schenk, P.; Pappalardo, R.

2003-12-01

The Geology subgroup of the Jupiter Icy Moons Orbiter (JIMO) Science Definition Team (SDT) has been working with colleagues within the planetary science community to determine the key outstanding science goals that could be met by the JIMO mission. Geological studies of the Galilean satellites will benefit from the spacecraft's long orbital periods around each satellite, lasting from one to several months. This mission plan allows us to select the optimal viewing conditions to complete global compositional and morphologic mapping at high resolution, and to target geologic features of key scientific interest at very high resolution. Community input to this planning process suggests two major science objectives, along with corresponding measurements proposed to meet them. Objective 1: Determine the origins of surface features and their implications for geological history and evolution. This encompasses investigations of magmatism (intrusion, extrusion, and diapirism), tectonism (isostatic compensation, and styles of faulting, flexure and folding), impact cratering (morphology and distribution), and gradation (erosion and deposition) processes (impact gardening, sputtering, mass wasting and frosts). Suggested measurements to meet this goal include (1) two dimensional global topographic mapping sufficient to discriminate features at a spatial scale of 10 m, and with better than or equal to 1 m relative vertical accuracy, (2) nested images of selected target areas at a range of resolutions down to the submeter pixel scale, (3) global (albedo) mapping at better than or equal to 10 m/pixel, and (4) multispectral global mapping in at least 3 colors at better than or equal to 100 m/pixel, with some subsets at better than 30 m/pixel. Objective 2. Identify and characterize potential landing sites for future missions. A primary component to the success of future landed missions is full characterization of potential sites in terms of their relative age, geological interest, and engineering safety. Measurement requirements suggested to meet this goal (in addition to the requirements of Objective 1) include the acquisition of super-high resolution images of selected target areas (with intermediate context imaging) down to 25 cm/pixel scale. The Geology subgroup passed these recommendations to the full JIMO Science Definition Team, to be incorporated into the final science recommendations for the JIMO mission.

Aerosol Optical Depth Retrieval With AVIRIS Data: A Test of Tafkaa

DTIC Science & Technology

2002-09-01

the spatial resolution . Clearly there is a need for a method of AOD retrieval that can cover more of the globe in a...imagers lack sufficient spectral resolution for some scientific applications. The future of remote sensing is in the ability to collect and interpret...AVIRIS is by using a data cube with two axes for the spatial dimensions and the third axis representing the 224 channels that make up the spectral

Achievement of a 920-MHz High Resolution NMR

NASA Astrophysics Data System (ADS)

Hashi, Kenjiro; Shimizu, Tadashi; Goto, Atsushi; Kiyoshi, Tsukasa; Matsumoto, Shinji; Wada, Hitoshi; Fujito, Teruaki; Hasegawa, Ken-ichi; Yoshikawa, Masatoshi; Miki, Takashi; Ito, Satoshi; Hamada, Mamoru; Hayashi, Seiji

2002-06-01

We have developed a 920-MHz NMR system and performed the proton NMR measurement of H 2O and ethylbenzene using the superconducting magnet operating at 21.6 T (920 MHz for proton), which is the highest field produced by a superconducting NMR magnet in the persistent mode. From the NMR measurements, it is verified that both homogeneity and stability of the magnet have a specification sufficient for a high resolution NMR.

Spectral Dimensionality and Scale of Urban Radiance

NASA Technical Reports Server (NTRS)

Small, Christopher

2001-01-01

Characterization of urban radiance and reflectance is important for understanding the effects of solar energy flux on the urban environment as well as for satellite mapping of urban settlement patterns. Spectral mixture analyses of Landsat and Ikonos imagery suggest that the urban radiance field can very often be described with combinations of three or four spectral endmembers. Dimensionality estimates of Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) radiance measurements of urban areas reveal the existence of 30 to 60 spectral dimensions. The extent to which broadband imagery collected by operational satellites can represent the higher dimensional mixing space is a function of both the spatial and spectral resolution of the sensor. AVIRIS imagery offers the spatial and spectral resolution necessary to investigate the scale dependence of the spectral dimensionality. Dimensionality estimates derived from Minimum Noise Fraction (MNF) eigenvalue distributions show a distinct scale dependence for AVIRIS radiance measurements of Milpitas, California. Apparent dimensionality diminishes from almost 40 to less than 10 spectral dimensions between scales of 8000 m and 300 m. The 10 to 30 m scale of most features in urban mosaics results in substantial spectral mixing at the 20 m scale of high altitude AVIRIS pixels. Much of the variance at pixel scales is therefore likely to result from actual differences in surface reflectance at pixel scales. Spatial smoothing and spectral subsampling of AVIRIS spectra both result in substantial loss of information and reduction of apparent dimensionality, but the primary spectral endmembers in all cases are analogous to those found in global analyses of Landsat and Ikonos imagery of other urban areas.

Ophthalmologic diagnostic tool using MR images for biomechanically-based muscle volume deformation

NASA Astrophysics Data System (ADS)

Buchberger, Michael; Kaltofen, Thomas

2003-05-01

We would like to give a work-in-progress report on our ophthalmologic diagnostic software system which performs biomechanically-based muscle volume deformations using MR images. For reconstructing a three-dimensional representation of an extraocular eye muscle, a sufficient amount of high resolution MR images is used, each representing a slice of the muscle. In addition, threshold values are given, which restrict the amount of data used from the MR images. The Marching Cube algorithm is applied to the polygons, resulting in a 3D representation of the muscle, which can efficiently be rendered. A transformation to a dynamic, deformable model is applied by calculating the center of gravity of each muscle slice, approximating the muscle path and subsequently adding Hermite splines through the centers of gravity of all slices. Then, a radius function is defined for each slice, completing the transformation of the static 3D polygon model. Finally, this paper describes future extensions to our system. One of these extensions is the support for additional calculations and measurements within the reconstructed 3D muscle representation. Globe translation, localization of muscle pulleys by analyzing the 3D reconstruction in two different gaze positions and other diagnostic measurements will be available.

SPIDER OPTIMIZATION. II. OPTICAL, MAGNETIC, AND FOREGROUND EFFECTS

DOE Office of Scientific and Technical Information (OSTI.GOV)

O'Dea, D. T.; Clark, C. N.; Contaldi, C. R.

2011-09-01

SPIDER is a balloon-borne instrument designed to map the polarization of the cosmic microwave background (CMB) with degree-scale resolution over a large fraction of the sky. SPIDER's main goal is to measure the amplitude of primordial gravitational waves through their imprint on the polarization of the CMB if the tensor-to-scalar ratio, r, is greater than 0.03. To achieve this goal, instrumental systematic errors must be controlled with unprecedented accuracy. Here, we build on previous work to use simulations of SPIDER observations to examine the impact of several systematic effects that have been characterized through testing and modeling of various instrumentmore » components. In particular, we investigate the impact of the non-ideal spectral response of the half-wave plates, coupling between focal-plane components and Earth's magnetic field, and beam mismatches and asymmetries. We also present a model of diffuse polarized foreground emission based on a three-dimensional model of the Galactic magnetic field and dust, and study the interaction of this foreground emission with our observation strategy and instrumental effects. We find that the expected level of foreground and systematic contamination is sufficiently low for SPIDER to achieve its science goals.« less

Effect of laser pulsing on the composition measurement of an Al-Mg-Si-Cu alloy using three-dimensional atom probe.

PubMed

Sha, G; Ringer, S P

2009-04-01

The effect of laser pulse energy on the composition measurement of an Al-Mg-Si-Cu alloy (AA6111) specimen has been investigated over a base temperature range of 20-80K and a voltage range of 2.5-5kV. Laser pulse energy must be sufficiently higher to achieve pulse-controlled field evaporation, which is at least 0.9nJ with a beam spot size of about 5microm, providing an equivalent voltage pulse fraction, approximately 14% at 80K for the alloy specimen. In contrast to the cluster composition, the measured specimen composition is sensitive to base temperature and laser energy changes. The exchange charge state under the influence of laser pulsing makes the detection of Si better at low base temperature, but detection of Cr and Mn is better at a higher temperature and using higher laser energy. No such effect occurs for detection of Mg and Cu under laser pulsing, although Mg concentration is sensitive to the analysis temperature under voltage pulsing. Mass resolution at full-width half-maximum is sensitive to local taper angle near the apex, but has little effect on composition measurement.

3D-analysis of plant microstructures: advantages and limitations of synchrotron X-ray microtomography

NASA Astrophysics Data System (ADS)

Matsushima, U.; Graf, W.; Zabler, S.; Manke, I.; Dawson, M.; Choinka, G.; Hilger, A.; Herppich, W. B.

2013-01-01

Synchrotron X-ray computer microtomography was used to analyze the microstructure of rose peduncles. Samples from three rose cultivars, differing in anatomy, were scanned to study the relation between tissue structure and peduncles mechanical strength. Additionally, chlorophyll fluorescence imaging and conventional light microscopy was applied to quantify possible irradiation-induced damage to plant physiology and tissue structure. The spatial resolution of synchrotron X-ray computer microtomography was sufficiently high to investigate the complex tissues of intact rose peduncles without the necessity of any preparation. However, synchrotron X-radiation induces two different types of damage on irradiated tissues. First, within a few hours after first X-ray exposure, there is a direct physical destruction of cell walls. In addition, a slow and delayed destruction of chlorophyll and, consequently, of photosynthetic activity occurred within hours/ days after the exposure. The results indicate that synchrotron X-ray computer microtomography is well suited for three-dimensional visualization of the microstructure of rose peduncles. However, in its current technique, synchrotron X-ray computer microtomography is not really non-destructive but induce tissue damage. Hence, this technique needs further optimization before it can be applied for time-series investigations of living plant materials

Classifying bilinear differential equations by linear superposition principle

NASA Astrophysics Data System (ADS)

Zhang, Lijun; Khalique, Chaudry Masood; Ma, Wen-Xiu

2016-09-01

In this paper, we investigate the linear superposition principle of exponential traveling waves to construct a sub-class of N-wave solutions of Hirota bilinear equations. A necessary and sufficient condition for Hirota bilinear equations possessing this specific sub-class of N-wave solutions is presented. We apply this result to find N-wave solutions to the (2+1)-dimensional KP equation, a (3+1)-dimensional generalized Kadomtsev-Petviashvili (KP) equation, a (3+1)-dimensional generalized BKP equation and the (2+1)-dimensional BKP equation. The inverse question, i.e., constructing Hirota Bilinear equations possessing N-wave solutions, is considered and a refined 3-step algorithm is proposed. As examples, we construct two very general kinds of Hirota bilinear equations of order 4 possessing N-wave solutions among which one satisfies dispersion relation and another does not satisfy dispersion relation.

A two-dimensional analysis of the sensitivity of a pulse first break to wave speed contrast on a scale below the resolution length of ray tomography.

PubMed

Willey, Carson L; Simonetti, Francesco

2016-06-01

Mapping the speed of mechanical waves traveling inside a medium is a topic of great interest across many fields from geoscience to medical diagnostics. Much work has been done to characterize the fidelity with which the geometrical features of the medium can be reconstructed and multiple resolution criteria have been proposed depending on the wave-matter interaction model used to decode the wave speed map from scattering measurements. However, these criteria do not define the accuracy with which the wave speed values can be reconstructed. Using two-dimensional simulations, it is shown that the first-arrival traveltime predicted by ray theory can be an accurate representation of the arrival of a pulse first break even in the presence of diffraction and other phenomena that are not accounted for by ray theory. As a result, ray-based tomographic inversions can yield accurate wave speed estimations also when the size of a sound speed anomaly is smaller than the resolution length of the inversion method provided that traveltimes are estimated from the signal first break. This increased sensitivity however renders the inversion more susceptible to noise since the amplitude of the signal around the first break is typically low especially when three-dimensional anomalies are considered.

High spatial resolution diffusion weighted imaging on clinical 3 T MRI scanners using multislab spiral acquisitions

PubMed Central

Holtrop, Joseph L.; Sutton, Bradley P.

2016-01-01

Abstract. A diffusion weighted imaging (DWI) approach that is signal-to-noise ratio (SNR) efficient and can be applied to achieve sub-mm resolutions on clinical 3 T systems was developed. The sequence combined a multislab, multishot pulsed gradient spin echo diffusion scheme with spiral readouts for imaging data and navigators. Long data readouts were used to keep the number of shots, and hence total imaging time, for the three-dimensional acquisition short. Image quality was maintained by incorporating a field-inhomogeneity-corrected image reconstruction to remove distortions associated with long data readouts. Additionally, multiple shots were required for the high-resolution images, necessitating motion induced phase correction through the use of efficiently integrated navigator data. The proposed approach is compared with two-dimensional (2-D) acquisitions that use either a spiral or a typical echo-planar imaging (EPI) acquisition to demonstrate the improved SNR efficiency. The proposed technique provided 71% higher SNR efficiency than the standard 2-D EPI approach. The adaptability of the technique to achieve high spatial resolutions is demonstrated by acquiring diffusion tensor imaging data sets with isotropic resolutions of 1.25 and 0.8 mm. The proposed approach allows for SNR-efficient sub-mm acquisitions of DWI data on clinical 3 T systems. PMID:27088107

Three-Dimensional Terahertz Coded-Aperture Imaging Based on Single Input Multiple Output Technology.

PubMed

Chen, Shuo; Luo, Chenggao; Deng, Bin; Wang, Hongqiang; Cheng, Yongqiang; Zhuang, Zhaowen

2018-01-19

As a promising radar imaging technique, terahertz coded-aperture imaging (TCAI) can achieve high-resolution, forward-looking, and staring imaging by producing spatiotemporal independent signals with coded apertures. In this paper, we propose a three-dimensional (3D) TCAI architecture based on single input multiple output (SIMO) technology, which can reduce the coding and sampling times sharply. The coded aperture applied in the proposed TCAI architecture loads either purposive or random phase modulation factor. In the transmitting process, the purposive phase modulation factor drives the terahertz beam to scan the divided 3D imaging cells. In the receiving process, the random phase modulation factor is adopted to modulate the terahertz wave to be spatiotemporally independent for high resolution. Considering human-scale targets, images of each 3D imaging cell are reconstructed one by one to decompose the global computational complexity, and then are synthesized together to obtain the complete high-resolution image. As for each imaging cell, the multi-resolution imaging method helps to reduce the computational burden on a large-scale reference-signal matrix. The experimental results demonstrate that the proposed architecture can achieve high-resolution imaging with much less time for 3D targets and has great potential in applications such as security screening, nondestructive detection, medical diagnosis, etc.

Three-dimensional estimates of tree canopies: Scaling from high-resolution UAV data to satellite observations

NASA Astrophysics Data System (ADS)

Sankey, T.; Donald, J.; McVay, J.

2015-12-01

High resolution remote sensing images and datasets are typically acquired at a large cost, which poses big a challenge for many scientists. Northern Arizona University recently acquired a custom-engineered, cutting-edge UAV and we can now generate our own images with the instrument. The UAV has a unique capability to carry a large payload including a hyperspectral sensor, which images the Earth surface in over 350 spectral bands at 5 cm resolution, and a lidar scanner, which images the land surface and vegetation in 3-dimensions. Both sensors represent the newest available technology with very high resolution, precision, and accuracy. Using the UAV sensors, we are monitoring the effects of regional forest restoration treatment efforts. Individual tree canopy width and height are measured in the field and via the UAV sensors. The high-resolution UAV images are then used to segment individual tree canopies and to derive 3-dimensional estimates. The UAV image-derived variables are then correlated to the field-based measurements and scaled to satellite-derived tree canopy measurements. The relationships between the field-based and UAV-derived estimates are then extrapolated to a larger area to scale the tree canopy dimensions and to estimate tree density within restored and control forest sites.

Linking rainforest ecophysiology and microclimate through fusion of airborne LiDAR and hyperspectral imagery

Treesearch

Eben N. Broadbent; Angélica M. Almeyda Zambrano; Gregory P. Asner; Christopher B. Field; Brad E. Rosenheim; Ty Kennedy-Bowdoin; David E. Knapp; David Burke; Christian Giardina; Susan Cordell

2014-01-01

We develop and validate a high-resolution three-dimensional model of light and air temperature for a tropical forest interior in Hawaii along an elevation gradient varying greatly in structure but maintaining a consistent species composition. Our microclimate models integrate high-resolution airborne waveform light detection and ranging data (LiDAR) and hyperspectral...

Compositions comprising free-standing two-dimensional nanocrystals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Barsoum, Michel W.; Gogotsi, Yury; Abdelmalak, Michael Naguib

2017-12-05

The present invention is directed to methods of transferring urea from an aqueous solution comprising urea to a MXene composition, the method comprising contacting the aqueous solution comprising urea with the MXene composition for a time sufficient to form an intercalated MXene composition comprising urea.

Explorations in Context Space: Words, Sentences, Discourse.

ERIC Educational Resources Information Center

Burgess, Curt; Livesay, Kay; Lund, Kevin

1998-01-01

Describes a computational model of high-dimensional context space: the Hyperspace Analog to Language (HAL). Shows that HAL provides sufficient information to make semantic, grammatical, and abstract distinctions. Demonstrates the cognitive compatibility of the representations with human processing; and introduces a new methodology that extracts…

Emergent behaviors of the Schrödinger-Lohe model on cooperative-competitive networks

NASA Astrophysics Data System (ADS)

Huh, Hyungjin; Ha, Seung-Yeal; Kim, Dohyun

2017-12-01

We present several sufficient frameworks leading to the emergent behaviors of the coupled Schrödinger-Lohe (S-L) model under the same one-body external potential on cooperative-competitive networks. The S-L model was first introduced as a possible phenomenological model exhibiting quantum synchronization and its emergent dynamics on all-to-all cooperative networks has been treated via two distinct approaches, Lyapunov functional approach and the finite-dimensional reduction based on pairwise correlations. In this paper, we further generalize the finite-dimensional dynamical systems approach for pairwise correlation functions on cooperative-competitive networks and provide several sufficient frameworks leading to the collective exponential synchronization. For small systems consisting of three and four quantum subsystem, we also show that the system for pairwise correlations can be reduced to the Lotka-Volterra model with cooperative and competitive interactions, in which lots of interesting dynamical patterns appear, e.g., existence of closed orbits and limit-cycles.

A comparison of sorptive extraction techniques coupled to a new quantitative, sensitive, high throughput GC-MS/MS method for methoxypyrazine analysis in wine.

PubMed

Hjelmeland, Anna K; Wylie, Philip L; Ebeler, Susan E

2016-02-01

Methoxypyrazines are volatile compounds found in plants, microbes, and insects that have potent vegetal and earthy aromas. With sensory detection thresholds in the low ng L(-1) range, modest concentrations of these compounds can profoundly impact the aroma quality of foods and beverages, and high levels can lead to consumer rejection. The wine industry routinely analyzes the most prevalent methoxypyrazine, 2-isobutyl-3-methoxypyrazine (IBMP), to aid in harvest decisions, since concentrations decrease during berry ripening. In addition to IBMP, three other methoxypyrazines IPMP (2-isopropyl-3-methoxypyrazine), SBMP (2-sec-butyl-3-methoxypyrazine), and EMP (2-ethyl-3-methoxypyrazine) have been identified in grapes and/or wine and can impact aroma quality. Despite their routine analysis in the wine industry (mostly IBMP), accurate methoxypyrazine quantitation is hindered by two major challenges: sensitivity and resolution. With extremely low sensory detection thresholds (~8-15 ng L(-1) in wine for IBMP), highly sensitive analytical methods to quantify methoxypyrazines at trace levels are necessary. Here we were able to achieve resolution of IBMP as well as IPMP, EMP, and SBMP from co-eluting compounds using one-dimensional chromatography coupled to positive chemical ionization tandem mass spectrometry. Three extraction techniques HS-SPME (headspace-solid phase microextraction), SBSE (stirbar sorptive extraction), and HSSE (headspace sorptive extraction) were validated and compared. A 30 min extraction time was used for HS-SPME and SBSE extraction techniques, while 120 min was necessary to achieve sufficient sensitivity for HSSE extractions. All extraction methods have limits of quantitation (LOQ) at or below 1 ng L(-1) for all four methoxypyrazines analyzed, i.e., LOQ's at or below reported sensory detection limits in wine. The method is high throughput, with resolution of all compounds possible with a relatively rapid 27 min GC oven program. Copyright © 2015 Elsevier B.V. All rights reserved.

Testing the skill of numerical hydraulic modeling to simulate spatiotemporal flooding patterns in the Logone floodplain, Cameroon

NASA Astrophysics Data System (ADS)

Fernández, Alfonso; Najafi, Mohammad Reza; Durand, Michael; Mark, Bryan G.; Moritz, Mark; Jung, Hahn Chul; Neal, Jeffrey; Shastry, Apoorva; Laborde, Sarah; Phang, Sui Chian; Hamilton, Ian M.; Xiao, Ningchuan

2016-08-01

Recent innovations in hydraulic modeling have enabled global simulation of rivers, including simulation of their coupled wetlands and floodplains. Accurate simulations of floodplains using these approaches may imply tremendous advances in global hydrologic studies and in biogeochemical cycling. One such innovation is to explicitly treat sub-grid channels within two-dimensional models, given only remotely sensed data in areas with limited data availability. However, predicting inundated area in floodplains using a sub-grid model has not been rigorously validated. In this study, we applied the LISFLOOD-FP hydraulic model using a sub-grid channel parameterization to simulate inundation dynamics on the Logone River floodplain, in northern Cameroon, from 2001 to 2007. Our goal was to determine whether floodplain dynamics could be simulated with sufficient accuracy to understand human and natural contributions to current and future inundation patterns. Model inputs in this data-sparse region include in situ river discharge, satellite-derived rainfall, and the shuttle radar topography mission (SRTM) floodplain elevation. We found that the model accurately simulated total floodplain inundation, with a Pearson correlation coefficient greater than 0.9, and RMSE less than 700 km2, compared to peak inundation greater than 6000 km2. Predicted discharge downstream of the floodplain matched measurements (Nash-Sutcliffe efficiency of 0.81), and indicated that net flow from the channel to the floodplain was modeled accurately. However, the spatial pattern of inundation was not well simulated, apparently due to uncertainties in SRTM elevations. We evaluated model results at 250, 500 and 1000-m spatial resolutions, and found that results are insensitive to spatial resolution. We also compared the model output against results from a run of LISFLOOD-FP in which the sub-grid channel parameterization was disabled, finding that the sub-grid parameterization simulated more realistic dynamics. These results suggest that analysis of global inundation is feasible using a sub-grid model, but that spatial patterns at sub-kilometer resolutions still need to be adequately predicted.

High resolution MRI imaging at 9.4 Tesla of the osteochondral unit in a translational model of articular cartilage repair.

PubMed

Goebel, Lars; Müller, Andreas; Bücker, Arno; Madry, Henning

2015-04-16

Non-destructive structural evaluation of the osteochondral unit is challenging. Here, the capability of high-field magnetic resonance imaging (μMRI) at 9.4 Tesla (T) was explored to examine osteochondral repair ex vivo in a preclinical large animal model. A specific aim of this study was to detect recently described alterations of the subchondral bone associated with cartilage repair. Osteochondral samples of medial femoral condyles from adult ewes containing full-thickness articular cartilage defects treated with marrow stimulation were obtained after 6 month in vivo and scanned in a 9.4 T μMRI. Ex vivo imaging of small osteochondral samples (typical volume: 1-2 cm(3)) at μMRI was optimised by variation of repetition time (TR), time echo (TE), flip angle (FA), spatial resolution and number of excitations (NEX) from standard MultiSliceMultiEcho (MSME) and three-dimensional (3D) spoiled GradientEcho (SGE) sequences. A 3D SGE sequence with the parameters: TR = 10 ms, TE = 3 ms, FA = 10°, voxel size = 120 × 120 × 120 μm(3) and NEX = 10 resulted in the best fitting for sample size, image quality, scanning time and artifacts. An isovolumetric voxel shape allowed for multiplanar reconstructions. Within the osteochondral unit articular cartilage, cartilaginous repair tissue and bone marrow could clearly be distinguished from the subchondral bone plate and subarticular spongiosa. Specific alterations of the osteochondral unit associated with cartilage repair such as persistent drill holes, subchondral bone cysts, sclerosis of the subchondral bone plate and of the subarticular spongiosa and intralesional osteophytes were precisely detected. High resolution, non-destructive ex vivo analysis of the entire osteochondral unit in a preclinical large animal model that is sufficient for further analyses is possible using μMRI at 9.4 T. In particular, 9.4 T is capable of accurately depicting alterations of the subchondral bone that are associated with osteochondral repair.

Modelling large-scale ice-sheet-climate interactions at the last glacial inception

NASA Astrophysics Data System (ADS)

Browne, O. J. H.; Gregory, J. M.; Payne, A. J.; Ridley, J. K.; Rutt, I. C.

2010-05-01

In order to investigate the interactions between coevolving climate and ice-sheets on multimillenial timescales, a low-resolution atmosphere-ocean general circulation model (AOGCM) has been coupled to a three-dimensional thermomechanical ice-sheet model. We use the FAMOUS AOGCM, which is almost identical in formulation to the widely used HadCM3 AOGCM, but on account of its lower resolution (7.5° longitude × 5° latitude in the atmosphere, 3.75°× 2.5° in the ocean) it runs about ten times faster. We use the community ice-sheet model Glimmer at 20 km resolution, with the shallow ice approximation and an annual degree-day scheme for surface mass balance. With the FAMOUS-Glimmer coupled model, we have simulated the growth of the Laurentide and Fennoscandian ice sheets at the last glacial inception, under constant orbital forcing and atmospheric composition for 116 ka BP. Ice grows in both regions, totalling 5.8 m of sea-level equivalent in 10 ka, slower than proxy records suggest. Positive climate feedbacks reinforce this growth at local scales (order hundreds of kilometres), where changes are an order of magnitude larger than on the global average. The albedo feedback (higher local albedo means a cooler climate) is important in the initial expansion of the ice-sheet area. The topography feedback (higher surface means a cooler climate) affects ice-sheet thickness and is not noticeable for the first 1 ka. These two feedbacks reinforce each other. Without them, the ice volume is ~90% less after 10 ka. In Laurentia, ice expands initially on the Canadian Arctic islands. The glaciation of the islands eventually cools the nearby mainland climate sufficiently to produce a positive mass balance there. Adjacent to the ice-sheets, cloud feedbacks tend to reduce the surface mass balance and restrain ice growth; this is an example of a local feedback whose simulation requires a model that includes detailed atmospheric physics.

Efficient non-hydrostatic modelling of 3D wave-induced currents using a subgrid approach

NASA Astrophysics Data System (ADS)

Rijnsdorp, Dirk P.; Smit, Pieter B.; Zijlema, Marcel; Reniers, Ad J. H. M.

2017-08-01

Wave-induced currents are an ubiquitous feature in coastal waters that can spread material over the surf zone and the inner shelf. These currents are typically under resolved in non-hydrostatic wave-flow models due to computational constraints. Specifically, the low vertical resolutions adequate to describe the wave dynamics - and required to feasibly compute at the scales of a field site - are too coarse to account for the relevant details of the three-dimensional (3D) flow field. To describe the relevant dynamics of both wave and currents, while retaining a model framework that can be applied at field scales, we propose a two grid approach to solve the governing equations. With this approach, the vertical accelerations and non-hydrostatic pressures are resolved on a relatively coarse vertical grid (which is sufficient to accurately resolve the wave dynamics), whereas the horizontal velocities and turbulent stresses are resolved on a much finer subgrid (of which the resolution is dictated by the vertical scale of the mean flows). This approach ensures that the discrete pressure Poisson equation - the solution of which dominates the computational effort - is evaluated on the coarse grid scale, thereby greatly improving efficiency, while providing a fine vertical resolution to resolve the vertical variation of the mean flow. This work presents the general methodology, and discusses the numerical implementation in the SWASH wave-flow model. Model predictions are compared with observations of three flume experiments to demonstrate that the subgrid approach captures both the nearshore evolution of the waves, and the wave-induced flows like the undertow profile and longshore current. The accuracy of the subgrid predictions is comparable to fully resolved 3D simulations - but at much reduced computational costs. The findings of this work thereby demonstrate that the subgrid approach has the potential to make 3D non-hydrostatic simulations feasible at the scale of a realistic coastal region.

Image formation analysis and high resolution image reconstruction for plenoptic imaging systems.

PubMed

Shroff, Sapna A; Berkner, Kathrin

2013-04-01

Plenoptic imaging systems are often used for applications like refocusing, multimodal imaging, and multiview imaging. However, their resolution is limited to the number of lenslets. In this paper we investigate paraxial, incoherent, plenoptic image formation, and develop a method to recover some of the resolution for the case of a two-dimensional (2D) in-focus object. This enables the recovery of a conventional-resolution, 2D image from the data captured in a plenoptic system. We show simulation results for a plenoptic system with a known response and Gaussian sensor noise.

Resolution enhancement using simultaneous couple illumination

NASA Astrophysics Data System (ADS)

Hussain, Anwar; Martínez Fuentes, José Luis

2016-10-01

A super-resolution technique based on structured illumination created by a liquid crystal on silicon spatial light modulator (LCOS-SLM) is presented. Single and simultaneous pairs of tilted beams are generated to illuminate a target object. Resolution enhancement of an optical 4f system is demonstrated by using numerical simulations. The resulting intensity images are recorded at a charged couple device (CCD) and stored in the computer memory for further processing. One dimension enhancement can be performed with only 15 images. Two dimensional complete improvement requires 153 different images. The resolution of the optical system is extended three times compared to the band limited system.

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

DOE PAGES

Carlton, Holly D.; Elmer, John W.; Li, Yan; ...

2016-04-13

For this study synchrotron radiation micro-­tomography, a non-destructive three-dimensional imaging technique, is employed to investigate an entire microelectronic package with a cross-sectional area of 16 x 16 mm. Due to the synchrotron’s high flux and brightness the sample was imaged in just 3 minutes with an 8.7 μm spatial resolution.

Overview of Three-Dimensional Atomic-Resolution Holography and Imaging Techniques: Recent Advances in Local-Structure Science

NASA Astrophysics Data System (ADS)

Daimon, Hiroshi

2018-06-01

Local three-dimensional (3D) atomic arrangements without periodicity have not been able to be studied until recently. Recently, several holographies and related techniques have been developed to reveal the 3D atomic arrangement around specific atoms with no translational symmetry. This review gives an overview of these new local 3D atomic imaging techniques.

Photoionization Rate of Atomic Oxygen

NASA Astrophysics Data System (ADS)

Meier, R. R.; McLaughlin, B. M.; Warren, H. P.; Bishop, J.

2006-05-01

Accurate knowledge of the photoionization rate of atomic oxygen is important for the study and understanding of the ionospheres and emission processes of terrestrial, planetary, and cometary atmospheres. Past calculations of the photoionization rate have been carried out at various spectral resolutions, but none were at sufficiently high resolution to accommodate accidental resonances between solar emission lines and highly structured auto-ionization features in the photoionization cross section. A new version of the NRLEUV solar spectral irradiance model (at solar minimum) and a new model of the O photoionization cross section enable calculations at very high spectral resolution. We find unattenuated photoionization rates computed at 0.001 nm resolution are larger than those at moderate resolution (0.1 nm) by amounts approaching 20%. Allowing for attenuation in the terrestrial atmosphere, we find differences in photoionization rates computed at high and moderate resolution to vary with altitude, especially below 200 km where deviations of plus or minus 20% occur between the two cases.

A Microwave Pressure Sounder

NASA Technical Reports Server (NTRS)

Flower, D. A.; Peckham, G. E.

1978-01-01

An instrument to measure atmospheric pressure at the earth's surface from an orbiting satellite would be a valuable addition to the expanding inventory of remote sensors. The subject of this report is such an instrument - the Microwave Pressure Sounder (MPS). It is shown that global-ocean coverage is attainable with sufficient accuracy, resolution and observational frequency for meteorological, oceanographic and climate research applications. Surface pressure can be deduced from a measurement of the absorption by an atmospheric column at a frequency in the wing of the oxygen band centered on 60 GHz. An active multifrequency instrument is needed to make this measurement with sufficient accuracy. The selection of optimum operating frequencies is based upon accepted models of surface reflection, oxygen, water vapor and cloud absorption. Numerical simulation using a range of real atmospheres defined by radiosonde observations were used to validate the frequency selection procedure. Analyses are presented of alternative system configurations that define the balance between accuracy and achievable resolution.

High-resolution two-dimensional and three-dimensional modeling of wire grid polarizers and micropolarizer arrays

NASA Astrophysics Data System (ADS)

Vorobiev, Dmitry; Ninkov, Zoran

2017-11-01

Recent advances in photolithography allowed the fabrication of high-quality wire grid polarizers for the visible and near-infrared regimes. In turn, micropolarizer arrays (MPAs) based on wire grid polarizers have been developed and used to construct compact, versatile imaging polarimeters. However, the contrast and throughput of these polarimeters are significantly worse than one might expect based on the performance of large area wire grid polarizers or MPAs, alone. We investigate the parameters that affect the performance of wire grid polarizers and MPAs, using high-resolution two-dimensional and three-dimensional (3-D) finite-difference time-domain simulations. We pay special attention to numerical errors and other challenges that arise in models of these and other subwavelength optical devices. Our tests show that simulations of these structures in the visible and near-IR begin to converge numerically when the mesh size is smaller than ˜4 nm. The performance of wire grid polarizers is very sensitive to the shape, spacing, and conductivity of the metal wires. Using 3-D simulations of micropolarizer "superpixels," we directly study the cross talk due to diffraction at the edges of each micropolarizer, which decreases the contrast of MPAs to ˜200∶1.

Anisotropy of MHD Turbulence at Low Magnetic Reynolds Number

NASA Technical Reports Server (NTRS)

Zikanov, O.; Vorobev, A.; Thess, A.; Davidson, P. A.; Knaepen, B.

2004-01-01

Turbulent fluctuations in MHD flows are known to become dimensionally anisotropic under the action of a sufficiently strong magnetic field. We consider the technologically relevant case of low magnetic Reynolds number and apply the method of DNS of forced flow in a periodic box to generate velocity fields. The analysis based on different anisotropy characteristics shows that the dimensional anisotropy is virtually scale-independent. We also find that, except for the case of very strong magnetic field, the flow is componentally isotropic. Its kinetic energy is practically uniformly distributed among the velocity components.

An equivalent domain integral method in the two-dimensional analysis of mixed mode crack problems

NASA Technical Reports Server (NTRS)

Raju, I. S.; Shivakumar, K. N.

1990-01-01

An equivalent domain integral (EDI) method for calculating J-integrals for two-dimensional cracked elastic bodies is presented. The details of the method and its implementation are presented for isoparametric elements. The EDI method gave accurate values of the J-integrals for two mode I and two mixed mode problems. Numerical studies showed that domains consisting of one layer of elements are sufficient to obtain accurate J-integral values. Two procedures for separating the individual modes from the domain integrals are presented.

Three dimensional thermal pollution models. Volume 1: Review of mathematical formulations. [waste heat discharge from power plants and effects on ecosystems

NASA Technical Reports Server (NTRS)

Lee, S. S.; Sengupta, S.

1978-01-01

A mathematical model package for thermal pollution analyses and prediction is presented. These models, intended as user's manuals, are three dimensional and time dependent using the primitive equation approach. Although they have sufficient generality for application at sites with diverse topographical features; they also present specific instructions regarding data preparation for program execution and sample problems. The mathematical formulation of these models is presented including assumptions, approximations, governing equations, boundary and initial conditions, numerical method of solution, and same results.

On the existence of global solutions of the one-dimensional cubic NLS for initial data in the modulation space Mp,q (R)

NASA Astrophysics Data System (ADS)

Chaichenets, Leonid; Hundertmark, Dirk; Kunstmann, Peer; Pattakos, Nikolaos

2017-10-01

We prove global existence for the one-dimensional cubic nonlinear Schrödinger equation in modulation spaces Mp,p‧ for p sufficiently close to 2. In contrast to known results, [9] and [14], our result requires no smallness condition on initial data. The proof adapts a splitting method inspired by work of Vargas-Vega, Hyakuna-Tsutsumi and Grünrock to the modulation space setting and exploits polynomial growth of the free Schrödinger group on modulation spaces.

Necessary optimality conditions for infinite dimensional state constrained control problems

NASA Astrophysics Data System (ADS)

Frankowska, H.; Marchini, E. M.; Mazzola, M.

2018-06-01

This paper is concerned with first order necessary optimality conditions for state constrained control problems in separable Banach spaces. Assuming inward pointing conditions on the constraint, we give a simple proof of Pontryagin maximum principle, relying on infinite dimensional neighboring feasible trajectories theorems proved in [20]. Further, we provide sufficient conditions guaranteeing normality of the maximum principle. We work in the abstract semigroup setting, but nevertheless we apply our results to several concrete models involving controlled PDEs. Pointwise state constraints (as positivity of the solutions) are allowed.

Time and Frequency Dependent Behavior of a Two Dimensional Electron Gas at Long Wavelengths.

DTIC Science & Technology

1985-11-05

lated by techniques which are basically similar to those employed 5-10 T y c e sfor the three dimensional case. The dynamic properties have also received...2m, Vk 2re 2/k, where m and e are, respectively, the electron mass and charge, 1 = . Our system is imposed under an external perturbing potential of...the following form: H = 3’ Pk(t) Pk e i’t (2) where -. is the Fourier comp6nent of the external electric field sufficiently small as to permit the use

Instrumental resolution as a function of scattering angle and wavelength as exemplified for the POWGEN instrument

PubMed Central

Jacobs, Philipp; Houben, Andreas; Schweika, Werner; Tchougréeff, Andrei L.; Dronskowski, Richard

2017-01-01

The method of angular- and wavelength-dispersive (e.g. two-dimensional) Rietveld refinement is a new and emerging tool for the analysis of neutron diffraction data measured at time-of-flight instruments with large area detectors. Following the approach for one-dimensional refinements (using either scattering angle or time of flight), the first step at each beam time cycle is the calibration of the instrument including the determination of instrumental contributions to the peak shape variation to be expected for diffraction patterns measured by the users. The aim of this work is to provide the users with calibration files and – for the later Rietveld refinement of the measured data – with an instrumental resolution file (IRF). This article will elaborate on the necessary steps to generate such an IRF for the angular- and wavelength-dispersive case, exemplified for the POWGEN instrument. A dataset measured on a standard diamond sample is used to extract the profile function in the two-dimensional case. It is found that the variation of reflection width with 2θ and λ can be expressed by the standard equation used for evaluating the instrumental resolution, which yields a substantially more fundamental approach to the parameterization of the instrumental contribution to the peak shape. Geometrical considerations of the POWGEN instrument and sample effects lead to values for Δθ, Δt and ΔL that yield a very good match to the extracted FWHM values. In a final step the refinement results are compared with the one-dimensional, i.e. diffraction-focused, case. PMID:28656041

Visual analysis of mass cytometry data by hierarchical stochastic neighbour embedding reveals rare cell types.

PubMed

van Unen, Vincent; Höllt, Thomas; Pezzotti, Nicola; Li, Na; Reinders, Marcel J T; Eisemann, Elmar; Koning, Frits; Vilanova, Anna; Lelieveldt, Boudewijn P F

2017-11-23

Mass cytometry allows high-resolution dissection of the cellular composition of the immune system. However, the high-dimensionality, large size, and non-linear structure of the data poses considerable challenges for the data analysis. In particular, dimensionality reduction-based techniques like t-SNE offer single-cell resolution but are limited in the number of cells that can be analyzed. Here we introduce Hierarchical Stochastic Neighbor Embedding (HSNE) for the analysis of mass cytometry data sets. HSNE constructs a hierarchy of non-linear similarities that can be interactively explored with a stepwise increase in detail up to the single-cell level. We apply HSNE to a study on gastrointestinal disorders and three other available mass cytometry data sets. We find that HSNE efficiently replicates previous observations and identifies rare cell populations that were previously missed due to downsampling. Thus, HSNE removes the scalability limit of conventional t-SNE analysis, a feature that makes it highly suitable for the analysis of massive high-dimensional data sets.

Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography

DOE PAGES

Yu, Young-Sang; Farmand, Maryam; Kim, Chunjoong; ...

2018-03-02

Battery function is determined by the efficiency and reversibility of the electrochemical phase transformations at solid electrodes. The microscopic tools available to study the chemical states of matter with the required spatial resolution and chemical specificity are intrinsically limited when studying complex architectures by their reliance on two-dimensional projections of thick material. Here in this paper, we report the development of soft X-ray ptychographic tomography, which resolves chemical states in three dimensions at 11 nm spatial resolution. We study an ensemble of nano-plates of lithium iron phosphate extracted from a battery electrode at 50% state of charge. Using a setmore » of nanoscale tomograms, we quantify the electrochemical state and resolve phase boundaries throughout the volume of individual nanoparticles. These observations reveal multiple reaction points, intra-particle heterogeneity, and size effects that highlight the importance of multi-dimensional analytical tools in providing novel insight to the design of the next generation of high-performance devices.« less

New horizons for study of the cardiopulmonary and circulatory systems. [image reconstruction techniques

NASA Technical Reports Server (NTRS)

Wood, E. H.

1976-01-01

The paper discusses the development of computer-controlled three-dimensional reconstruction techniques designed to determine the dynamic changes in the true shape and dimensions of the epi- and endocardial surfaces of the heart, along with variable time base (stop-action to real-time) displays of the transmural distribution of the coronary microcirculation and the three-dimensional anatomy of the macrovasculature in all regions of the body throughout individual cardiac and/or respiratory cycles. A technique for reconstructing a cross section of the heart from multiplanar videoroentgenograms is outlined. The capability of high spatial and high temporal resolution scanning videodensitometry makes possible measurement of the appearance, mean transit and clearance of roentgen opaque substances in three-dimensional space through the myocardium with a degree of simultaneous anatomic and temporal resolution not obtainable by current isotope techniques. The distribution of a variety of selected chemical elements or biologic materials within a body portion can also be determined.

Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yu, Young-Sang; Farmand, Maryam; Kim, Chunjoong

Battery function is determined by the efficiency and reversibility of the electrochemical phase transformations at solid electrodes. The microscopic tools available to study the chemical states of matter with the required spatial resolution and chemical specificity are intrinsically limited when studying complex architectures by their reliance on two-dimensional projections of thick material. Here in this paper, we report the development of soft X-ray ptychographic tomography, which resolves chemical states in three dimensions at 11 nm spatial resolution. We study an ensemble of nano-plates of lithium iron phosphate extracted from a battery electrode at 50% state of charge. Using a setmore » of nanoscale tomograms, we quantify the electrochemical state and resolve phase boundaries throughout the volume of individual nanoparticles. These observations reveal multiple reaction points, intra-particle heterogeneity, and size effects that highlight the importance of multi-dimensional analytical tools in providing novel insight to the design of the next generation of high-performance devices.« less

A 4-Dimensional Representation of Antennal Lobe Output Based on an Ensemble of Characterized Projection Neurons

PubMed Central

Staudacher, Erich M.; Huetteroth, Wolf; Schachtner, Joachim; Daly, Kevin C.

2009-01-01

A central problem facing studies of neural encoding in sensory systems is how to accurately quantify the extent of spatial and temporal responses. In this study, we take advantage of the relatively simple and stereotypic neural architecture found in invertebrates. We combine standard electrophysiological techniques, recently developed population analysis techniques, and novel anatomical methods to form an innovative 4-dimensional view of odor output representations in the antennal lobe of the moth Manduca sexta. This novel approach allows quantification of olfactory responses of characterized neurons with spike time resolution. Additionally, arbitrary integration windows can be used for comparisons with other methods such as imaging. By assigning statistical significance to changes in neuronal firing, this method can visualize activity across the entire antennal lobe. The resulting 4-dimensional representation of antennal lobe output complements imaging and multi-unit experiments yet provides a more comprehensive and accurate view of glomerular activation patterns in spike time resolution. PMID:19464513

Sampling supraglacial debris thickness using terrestrial photogrammetry

NASA Astrophysics Data System (ADS)

Nicholson, Lindsey; Mertes, Jordan

2017-04-01

The melt rate of debris-covered ice differs to that of clean ice primarily as a function of debris thickness. The spatial distribution of supraglacial debris thickness must therefore be known in order to understand how it is likely to impact glacier behaviour, and meltwater contribution to local hydrological resources and global sea level rise. However, practical means of determining debris cover thickness remain elusive. In this study we explore the utility of terrestrial photogrammetry to produce high resolution, scaled and texturized digital terrain models of debris cover exposures above ice cliffs as a means of quantifying and characterizing debris thickness. Two Nikon D5000 DSLRs with Tamron 100mm lenses were used to photograph a sample area of the Ngozumpa glacier in the Khumbu Himal of Nepal in April 2016. A Structure from Motion workflow using Agisoft Photoscan software was used to generate a surface models with <10cm resolution. A Trimble Geo7X differential GPS with Zephyr antenna, along with a local base station, was used to precisely measure marked ground control points to scale the photogrammetric surface model. Measurements of debris thickness along the exposed cliffline were made from this scaled model, assuming that the ice surface at the debris-ice boundary is horizontal, and these data are compared to 50 manual point measurements along the same clifftops. We conclude that sufficiently high resolution photogrammetry, with precise scaling information, provides a useful means to determine debris thickness at clifftop exposures. The resolution of the possible measurements depends on image resolution, the accuracy of the ground control points and the computational capacity to generate centimetre scale surface models. Application of such techniques to sufficiently high resolution imagery from UAV-borne cameras may offer a powerful means of determining debris thickness distribution patterns over debris covered glacier termini.

A comparison of EEOC closures involving hiring versus other prevalent discrimination issues under the Americans with Disabilities Act.

PubMed

McMahon, Brian T; Hurley, Jessica E; West, Steven L; Chan, Fong; Roessler, Richard; Rumrill, Phillip D

2008-06-01

This article describes findings from a causal comparative study of the Merit Resolution rate for allegations of Hiring discrimination that were filed with the U.S. Equal Employment Opportunity Commission (EEOC) under Title I of the Americans with Disabilities Act (ADA) between 1992 and 2005. An allegation is the Charging Party's perception of discrimination, but a Merit Resolution is one in which the EEOC has determined that a discriminatory event did indeed occur. A Non-Merit Resolution is an allegation that is closed due to a technicality or lacks sufficient evidence to conclude that discrimination occurred. Merit favors the Charging Party; Non-Merit favors the Employer. The Merit Resolution rate of 19,527 closed Hiring allegations is compared and contrasted to that of 259,680 allegations aggregated from six other prevalent forms of discrimination including Discharge and Constructive Discharge, Reasonable Accommodation, Disability Harassment and Intimidation, and Terms and Conditions of Employment. Tests of Proportion distributed as chi-square are used to form comparisons along a variety of subcategories of Merit and Non-Merit outcomes. The overall Merit Resolution rate for Hiring is 26% compared to Non-Hiring at 20.6%. Employers are less likely to settle claims of hiring discrimination without mediation, and less likely to accept the remedies recommended by the EEOC when hiring discrimination has been determined. Hiring is not an unusual discrimination issue in that the overwhelming majority of allegations are still closed in favor of the Employer. However, it is counterintuitive that hiring has a higher merit resolution rate than other prevalent issues. This finding contradicts the assumption that hiring is an "invisible process." Considering that the EEOC makes merit determinations at a competitive rate, it is clear that hiring is sufficiently transparent.

Design and Development of a Megavoltage CT Scanner for Radiation Therapy.

NASA Astrophysics Data System (ADS)

Chen, Ching-Tai

A Varian 4 MeV isocentric therapy accelerator has been modified to perform also as a CT scanner. The goal is to provide low cost computed tomography capability for use in radiotherapy. The system will have three principal uses. These are (i) to provide 2- and 3-dimensional maps of electron density distribution for CT assisted therapy planning, (ii) to aid in patient set up by providing sectional views of the treatment volume and high contrast scout-mode verification images and (iii) to provide a means for periodically checking the patients anatomical conformation against what was used to generate the original therapy plan. The treatment machine was modified by mounting an array of detectors on a frame bolted to the counter weight end of the gantry in such a manner as to define a 'third generation' CT Scanner geometry. The data gathering is controlled by a Z-80 based microcomputer system which transfers the x-ray transmission data to a general purpose PDP 11/34 for processing. There a series of calibration processes and a logarithmic conversion are performed to get projection data. After reordering the projection data to an equivalent parallel beam sinogram format a convolution algorithm is employed to construct the image from the equivalent parallel projection data. Results of phantom studies have shown a spatial resolution of 2.6 mm and an electron density discrimination of less than 1% which are sufficiently good for accurate therapy planning. Results also show that the system is linear to within the precision of our measurement ((DBLTURN).75%) over a wide range of electron densities corresponding to those found in body tissues. Animal and human images are also presented to demonstrate that the system's imaging capability is sufficient to allow the necessary visualization of anatomy.

Guaranteed convergence of the Hough transform

NASA Astrophysics Data System (ADS)

Soffer, Menashe; Kiryati, Nahum

1995-01-01

The straight-line Hough Transform using normal parameterization with a continuous voting kernel is considered. It transforms the colinearity detection problem to a problem of finding the global maximum of a two dimensional function above a domain in the parameter space. The principle is similar to robust regression using fixed scale M-estimation. Unlike standard M-estimation procedures the Hough Transform does not rely on a good initial estimate of the line parameters: The global optimization problem is approached by exhaustive search on a grid that is usually as fine as computationally feasible. The global maximum of a general function above a bounded domain cannot be found by a finite number of function evaluations. Only if sufficient a-priori knowledge about the smoothness of the objective function is available, convergence to the global maximum can be guaranteed. The extraction of a-priori information and its efficient use are the main challenges in real global optimization problems. The global optimization problem in the Hough Transform is essentially how fine should the parameter space quantization be in order not to miss the true maximum. More than thirty years after Hough patented the basic algorithm, the problem is still essentially open. In this paper an attempt is made to identify a-priori information on the smoothness of the objective (Hough) function and to introduce sufficient conditions for the convergence of the Hough Transform to the global maximum. An image model with several application dependent parameters is defined. Edge point location errors as well as background noise are accounted for. Minimal parameter space quantization intervals that guarantee convergence are obtained. Focusing policies for multi-resolution Hough algorithms are developed. Theoretical support for bottom- up processing is provided. Due to the randomness of errors and noise, convergence guarantees are probabilistic.

Anti-aliasing techniques in photon-counting depth imaging using GHz clock rates

NASA Astrophysics Data System (ADS)

Krichel, Nils J.; McCarthy, Aongus; Collins, Robert J.; Buller, Gerald S.

2010-04-01

Single-photon detection technologies in conjunction with low laser illumination powers allow for the eye-safe acquisition of time-of-flight range information on non-cooperative target surfaces. We previously presented a photon-counting depth imaging system designed for the rapid acquisition of three-dimensional target models by steering a single scanning pixel across the field angle of interest. To minimise the per-pixel dwelling times required to obtain sufficient photon statistics for accurate distance resolution, periodic illumination at multi- MHz repetition rates was applied. Modern time-correlated single-photon counting (TCSPC) hardware allowed for depth measurements with sub-mm precision. Resolving the absolute target range with a fast periodic signal is only possible at sufficiently short distances: if the round-trip time towards an object is extended beyond the timespan between two trigger pulses, the return signal cannot be assigned to an unambiguous range value. Whereas constructing a precise depth image based on relative results may still be possible, problems emerge for large or unknown pixel-by-pixel separations or in applications with a wide range of possible scene distances. We introduce a technique to avoid range ambiguity effects in time-of-flight depth imaging systems at high average pulse rates. A long pseudo-random bitstream is used to trigger the illuminating laser. A cyclic, fast-Fourier supported analysis algorithm is used to search for the pattern within return photon events. We demonstrate this approach at base clock rates of up to 2 GHz with varying pattern lengths, allowing for unambiguous distances of several kilometers. Scans at long stand-off distances and of scenes with large pixel-to-pixel range differences are presented. Numerical simulations are performed to investigate the relative merits of the technique.

Food self-sufficiency across scales: how local can we go?

PubMed

Pradhan, Prajal; Lüdeke, Matthias K B; Reusser, Dominik E; Kropp, Juergen P

2014-08-19

This study explores the potential for regions to shift to a local food supply using food self-sufficiency (FSS) as an indicator. We considered a region food self-sufficient when its total calorie production is enough to meet its demand. For future scenarios, we considered population growth, dietary changes, improved feed conversion efficiency, climate change, and crop yield increments. Starting at the 5' resolution, we investigated FSS from the lowest administrative levels to continents. Globally, about 1.9 billion people are self-sufficient within their 5' grid, while about 1 billion people from Asia and Africa require cross-continental agricultural trade in 2000. By closing yield gaps, these regions can achieve FSS, which also reduces international trade and increases a self-sufficient population in a 5' grid to 2.9 billion. The number of people depending on international trade will vary between 1.5 and 6 billion by 2050. Climate change may increase the need for international agricultural trade by 4% to 16%.

The Two-Dimensional Gabor Function Adapted to Natural Image Statistics: A Model of Simple-Cell Receptive Fields and Sparse Structure in Images.

PubMed

Loxley, P N

2017-10-01

The two-dimensional Gabor function is adapted to natural image statistics, leading to a tractable probabilistic generative model that can be used to model simple cell receptive field profiles, or generate basis functions for sparse coding applications. Learning is found to be most pronounced in three Gabor function parameters representing the size and spatial frequency of the two-dimensional Gabor function and characterized by a nonuniform probability distribution with heavy tails. All three parameters are found to be strongly correlated, resulting in a basis of multiscale Gabor functions with similar aspect ratios and size-dependent spatial frequencies. A key finding is that the distribution of receptive-field sizes is scale invariant over a wide range of values, so there is no characteristic receptive field size selected by natural image statistics. The Gabor function aspect ratio is found to be approximately conserved by the learning rules and is therefore not well determined by natural image statistics. This allows for three distinct solutions: a basis of Gabor functions with sharp orientation resolution at the expense of spatial-frequency resolution, a basis of Gabor functions with sharp spatial-frequency resolution at the expense of orientation resolution, or a basis with unit aspect ratio. Arbitrary mixtures of all three cases are also possible. Two parameters controlling the shape of the marginal distributions in a probabilistic generative model fully account for all three solutions. The best-performing probabilistic generative model for sparse coding applications is found to be a gaussian copula with Pareto marginal probability density functions.

Display dimensionality and conflict geometry effects on maneuver preferences for resolving in-flight conflicts.

PubMed

Thomas, Lisa C; Wickens, Christopher D

2008-08-01

Two experiments explored the effects of display dimensionality, conflict geometry, and time pressure on pilot maneuvering preferences for resolving en route conflicts. With the presence of a cockpit display of traffic information (CDTI) that provides graphical airspace information, pilots can use a variety of conflict resolution maneuvers in response to how they perceive the conflict. Inconsistent preference findings from previous research on conflict resolution using CDTIs may be attributable to inherent ambiguities in 3-D perspective displays and/or a limited range of conflict geometries. Pilots resolved predicted conflicts using CDTIs with three levels of display dimensionality; the first had two 2-D orthogonal views, the second depicted the airspace in two alternating 3-D perspective views, and the third had a pilot-controlled swiveling viewpoint. Pilots demonstrated the same preferences that have been observed in previous research for vertical over lateral maneuvers in low workload and climbs over descents for level-flight conflicts. With increasing workload the two 3-D perspective displays, but not the 2-D displays, resulted in an increased preference for lateral over vertical maneuvers. Increased time pressure resulted in increased vertical maneuvers, an effect again limited to the two 3-D perspective displays. Resolution preferences were more affected by workload and time pressure when the 3-D perspective displays were used, as compared with the 2-D displays, although overall preferences were milder than in previous studies. Investigating maneuver preferences using the strategic flight planning paradigm employed in this study may be the key to better ensure pilot acceptance of computer-generated resolution maneuvers.

Nonoscillatory solutions for system of neutral dynamic equations on time scales.

PubMed

Chen, Zhanhe; Sun, Taixiang; Wang, Qi; Xi, Hongjian

2014-01-01

We will discuss nonoscillatory solutions to the n-dimensional functional system of neutral type dynamic equations on time scales. We will establish some sufficient conditions for nonoscillatory solutions with the property lim(t → ∞) x(i) (t) = 0, i = 1, 2,…, n.

Using Two-Dimensional and Perspective Views of Terrain

DTIC Science & Technology

2000-02-01

think of 2-D and 3-D displays as "natural concepts" (Rosch, 1973; Wittgenstein , 1958) that have no defining necessary or sufficient conditions...Psychology, vol. 6, 241-271. Wittgenstein , L. 1958. Philosophical Investigations. 2nd ed. Blackwell Publishers, Oxford, U.K. 15 REPORT DOCUMENTATION PAGE

Technique to quantitatively measure magnetic properties of thin structures at <10 NM spatial resolution

DOEpatents

Bajt, Sasa

2003-07-08

A highly sensitive and high resolution magnetic microscope images magnetic properties quantitatively. Imaging is done with a modified transmission electron microscope that allows imaging of the sample in a zero magnetic field. Two images from closely spaced planes, one in focus and one slightly out of focus, are sufficient to calculate the absolute values of the phase change imparted to the electrons, and hence obtain the magnetization vector field distribution.

Perceptual integration of kinematic components in the recognition of emotional facial expressions.

PubMed

Chiovetto, Enrico; Curio, Cristóbal; Endres, Dominik; Giese, Martin

2018-04-01

According to a long-standing hypothesis in motor control, complex body motion is organized in terms of movement primitives, reducing massively the dimensionality of the underlying control problems. For body movements, this low-dimensional organization has been convincingly demonstrated by the learning of low-dimensional representations from kinematic and EMG data. In contrast, the effective dimensionality of dynamic facial expressions is unknown, and dominant analysis approaches have been based on heuristically defined facial "action units," which reflect contributions of individual face muscles. We determined the effective dimensionality of dynamic facial expressions by learning of a low-dimensional model from 11 facial expressions. We found an amazingly low dimensionality with only two movement primitives being sufficient to simulate these dynamic expressions with high accuracy. This low dimensionality is confirmed statistically, by Bayesian model comparison of models with different numbers of primitives, and by a psychophysical experiment that demonstrates that expressions, simulated with only two primitives, are indistinguishable from natural ones. In addition, we find statistically optimal integration of the emotion information specified by these primitives in visual perception. Taken together, our results indicate that facial expressions might be controlled by a very small number of independent control units, permitting very low-dimensional parametrization of the associated facial expression.

High-resolution MR imaging for dental impressions: a feasibility study.

PubMed

Boldt, Julian; Rottner, Kurt; Schmitter, Marc; Hopfgartner, Andreas; Jakob, Peter; Richter, Ernst-Jürgen; Tymofiyeva, Olga

2018-04-01

Magnetic resonance imaging is an emerging technology in dental medicine. While low-resolution MRI has especially provided means to examine the temporomandibular joint due to its anatomic inaccessibility, it was the goal of this study to assess whether high-resolution MRI is capable of delivering a dataset sufficiently precise enough to serve as digital impression of human teeth. An informed and consenting patient in need of dental restoration with fixed partial dentures was chosen as subject. Two prepared teeth were measured using MRI and the dataset subjected to mathematical processing before Fourier transformation. After reconstruction, a 3D file was generated which was fed into an existing industry standard CAD/CAM process. A framework for a fixed dental prosthesis was digitally modeled and manufactured by laser-sintering. The fit in situ was found to be acceptable by current clinical standards, which allowed permanent placement of the fixed prosthesis. Using a clinical whole-body MR scanner with the addition of custom add-on hardware, contrast enhancement, and data post-processing, resolution and signal-to-noise ratio were sufficiently achieved to allow fabrication of a dental restoration in an acquisition time comparable to the setting time of common dental impression materials. Furthermore, the measurement was well tolerated. The herein described method can be regarded as proof of principle that MRI is a promising option for digital impressions when fixed partial dentures are required.