New implementation of a shear-force microscope suitable to study topographical features over wide areas

NASA Astrophysics Data System (ADS)

Ustione, A.; Cricenti, A.; Piacentini, M.; Felici, A. C.

2006-09-01

A new implementation of a shear-force microscope is described that uses a shear-force detection system to perform topographical imaging of large areas (˜1×1mm2). This implementation finds very interesting application in the study of archeological or artistic samples. Three dc motors are used to move a sample during a scan, allowing the probe tip to follow the surface and to face height differences of several tens of micrometers. This large-area topographical imaging mode exploits new subroutines that were added to the existing homemade software; these subroutines were created in Microsoft VISUAL BASIC 6.0 programming language. With this new feature our shear-force microscope can be used to study topographical details over large areas of archaeological samples in a nondestructive way. We show results detecting worn reliefs over a coin.

Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin

PubMed Central

Vitry, Pauline; Valotteau, Claire; Feuillie, Cécile; Bernard, Simon

2017-01-01

ABSTRACT Bacterial pathogens that colonize host surfaces are subjected to physical stresses such as fluid flow and cell surface contacts. How bacteria respond to such mechanical cues is an important yet poorly understood issue. Staphylococcus aureus uses a repertoire of surface proteins to resist shear stress during the colonization of host tissues, but whether their adhesive functions can be modulated by physical forces is not known. Here, we show that the interaction of S. aureus clumping factor B (ClfB) with the squamous epithelial cell envelope protein loricrin is enhanced by mechanical force. We find that ClfB mediates S. aureus adhesion to loricrin through weak and strong molecular interactions both in a laboratory strain and in a clinical isolate. Strong forces (~1,500 pN), among the strongest measured for a receptor-ligand bond, are consistent with a high-affinity “dock, lock, and latch” binding mechanism involving dynamic conformational changes in the adhesin. Notably, we demonstrate that the strength of the ClfB-loricrin bond increases as mechanical force is applied. These findings favor a two-state model whereby bacterial adhesion to loricrin is enhanced through force-induced conformational changes in the ClfB molecule, from a weakly binding folded state to a strongly binding extended state. This force-sensitive mechanism may provide S. aureus with a means to finely tune its adhesive properties during the colonization of host surfaces, helping cells to attach firmly under high shear stress and to detach and spread under low shear stress. PMID:29208742

A numerical study on high-pressure water-spray cleaning for CSP reflectors

NASA Astrophysics Data System (ADS)

Anglani, Francesco; Barry, John; Dekkers, Willem

2016-05-01

Mirror cleaning for concentrated solar thermal (CST) systems is an important aspect of operation and maintenance (O&M), which affects solar field efficiency. The cleaning process involves soil removal by erosion, resulting from droplet impingement on the surface. Several studies have been conducted on dust accumulation and CSP plant reflectivity restoration, demonstrating that parameters such as nozzle diameter, jet impingement angle, interaxial distance between nozzles, standoff distance, water velocity, nozzle pressure and others factors influence the extent of reflectance restoration. In this paper we aim at identifying optimized cleaning strategies suitable for CST plants, able to restore mirror reflectance by high-pressure water-spray systems through the enhancement of shear stress over reflectors' surface. In order to evaluate the forces generated by water-spray jet impingement during the cleaning process, fluid dynamics simulations have been undertaken with ANSYS CFX software. In this analysis, shear forces represent the "critical phenomena" within the soil removal process. Enhancing shear forces on a particular area of the target surface, varying the angle of impingement in combination with the variation of standoff distances, and managing the interaxial distance of nozzles can increase cleaning efficiency. This procedure intends to improve the cleaning operation for CST mirrors reducing spotted surface and increasing particles removal efficiency. However, turbulence developed by adjacent flows decrease the shear stress generated on the reflectors surface. The presence of turbulence is identified by the formation of "fountain regions" which are mostly responsible of cleaning inefficiency. By numerical analysis using ANSYS CFX, we have modelled a stationary water-spray system with an array of three nozzles in line, with two angles of impingement: θ = 90° and θ = 75°. Several numerical tests have been carried out, varying the interaxial distance of nozzles, standoff distance, jet pressure and jet impingement angle in order to identify effective and efficient cleaning procedures to restore collectors' reflectance, decrease turbulence and improve CST plant efficiency. Results show that the forces generated over the flat target surface are proportional to the inlet pressure and to the water velocity over the surface, and that the shear stresses decrease as the standoff distance increases.

All-Elastomer 3-Axis Contact Resistive Tactile Sensor Arrays and Micromilled Manufacturing Methods Thereof

NASA Technical Reports Server (NTRS)

Penskiy, Ivan (Inventor); Charalambides, Alexandros (Inventor); Bergbreiter, Sarah (Inventor)

2018-01-01

At least one tactile sensor includes an insulating layer and a conductive layer formed on the surface of the insulating layer. The conductive layer defines at least one group of flexible projections extending orthogonally from the surface of the insulating layer. The flexible projections include a major projection extending a distance orthogonally from the surface and at least one minor projection that is adjacent to and separate from the major projection wherein the major projection extends a distance orthogonally that is greater than the distance that the minor projection extends orthogonally. Upon a compressive force normal to, or a shear force parallel to, the surface, the major projection and the minor projection flex such that an electrical contact resistance is formed between the major projection and the minor projection. A capacitive tactile sensor is also disclosed that responds to the normal and shear forces.

The effect of dry shear aligning of nanotube thin films on the photovoltaic performance of carbon nanotube-silicon solar cells.

PubMed

Stolz, Benedikt W; Tune, Daniel D; Flavel, Benjamin S

2016-01-01

Recent results in the field of carbon nanotube-silicon solar cells have suggested that the best performance is obtained when the nanotube film provides good coverage of the silicon surface and when the nanotubes in the film are aligned parallel to the surface. The recently developed process of dry shear aligning - in which shear force is applied to the surface of carbon nanotube thin films in the dry state, has been shown to yield nanotube films that are very flat and in which the surface nanotubes are very well aligned in the direction of shear. It is thus reasonable to expect that nanotube films subjected to dry shear aligning should outperform otherwise identical films formed by other processes. In this work, the fabrication and characterisation of carbon nanotube-silicon solar cells using such films is reported, and the photovoltaic performance of devices produced with and without dry shear aligning is compared.

The effect of dry shear aligning of nanotube thin films on the photovoltaic performance of carbon nanotube–silicon solar cells

PubMed Central

Stolz, Benedikt W; Tune, Daniel D

2016-01-01

Summary Recent results in the field of carbon nanotube–silicon solar cells have suggested that the best performance is obtained when the nanotube film provides good coverage of the silicon surface and when the nanotubes in the film are aligned parallel to the surface. The recently developed process of dry shear aligning – in which shear force is applied to the surface of carbon nanotube thin films in the dry state, has been shown to yield nanotube films that are very flat and in which the surface nanotubes are very well aligned in the direction of shear. It is thus reasonable to expect that nanotube films subjected to dry shear aligning should outperform otherwise identical films formed by other processes. In this work, the fabrication and characterisation of carbon nanotube–silicon solar cells using such films is reported, and the photovoltaic performance of devices produced with and without dry shear aligning is compared. PMID:27826524

Shear Stress Partitioning in Large Patches of Roughness in the Atmospheric Inertial Sublayer

NASA Technical Reports Server (NTRS)

Gillies, John A.; Nickling, William G.; King, James

2007-01-01

Drag partition measurements were made in the atmospheric inertial sublayer for six roughness configurations made up of solid elements in staggered arrays of different roughness densities. The roughness was in the form of a patch within a large open area and in the shape of an equilateral triangle with 60 m long sides. Measurements were obtained of the total shear stress (tau) acting on the surfaces, the surface shear stress on the ground between the elements (tau(sub S)) and the drag force on the elements for each roughness array. The measurements indicated that tau(sub S) quickly reduced near the leading edge of the roughness compared with tau, and a tau(sub S) minimum occurs at a normalized distance (x/h, where h is element height) of approx. -42 (downwind of the roughness leading edge is negative), then recovers to a relatively stable value. The location of the minimum appears to scale with element height and not roughness density. The force on the elements decreases exponentially with normalized downwind distance and this rate of change scales with the roughness density, with the rate of change increasing as roughness density increases. Average tau(sub S): tau values for the six roughness surfaces scale predictably as a function of roughness density and in accordance with a shear stress partitioning model. The shear stress partitioning model performed very well in predicting the amount of surface shear stress, given knowledge of the stated input parameters for these patches of roughness. As the shear stress partitioning relationship within the roughness appears to come into equilibrium faster for smaller roughness element sizes it would also appear the shear stress partitioning model can be applied with confidence for smaller patches of smaller roughness elements than those used in this experiment.

Surface shear stress dependence of gas transfer velocity parameterizations using DNS

NASA Astrophysics Data System (ADS)

Fredriksson, S. T.; Arneborg, L.; Nilsson, H.; Handler, R. A.

2016-10-01

Air-water gas-exchange is studied in direct numerical simulations (DNS) of free-surface flows driven by natural convection and weak winds. The wind is modeled as a constant surface-shear-stress and the gas-transfer is modeled via a passive scalar. The simulations are characterized via a Richardson number Ri=Bν/u*4 where B, ν, and u* are the buoyancy flux, kinematic viscosity, and friction velocity respectively. The simulations comprise 0Ric or kg=AShearu*Sc-n, Ri

Control of Attachment of Pseudomonas aeruginosa and Burkholderia cepacia to Surfaces by Shear Force.

PubMed

Hui, Yew Woh; Narayanan, Kumaran; Dykes, Gary A

2016-11-01

  The effect of physical shearing on the attachment of six Pseudomonas aeruginosa strains and six Burkholderia cepacia strains to glass, stainless steel, polystyrene and Teflon® was determined. A significant (p < 0.05) decrease in hydrophobicity was apparent for all P. aeruginosa strains (17-36%) and B. cepacia, MS 5 (20%) after shearing. A significant (p < 0.05) decrease in attachment of some P. aeruginosa (0.2-0.5 log CFU/cm2) and B. cepacia (0.2-0.4 log CFU/cm2) strains to some surface types was apparent after shearing. Significant (p < 0.05) correlation was observed for both numbers of flagellated cells and hydrophobicity against attachment to glass, stainless steel and polystyrene for P. aeruginosa while only hydrophobicity showed significant correlation against the same surfaces for B. cepacia. Scanning electron microscopy and protein analysis showed that shearing removed surface proteins from the cells and may have led to the observed changes in hydrophobicity and attachment to abiotic surfaces.

Some New Problems on Shells and Thin Structures

NASA Technical Reports Server (NTRS)

Vlasov, V. S.

1949-01-01

Cylindrical shells of arbitrary section, reinforced by longitudinal and transverse members (stringers and ribs) are considered by us, for a sufficiently close spacing of the ribs, as in our previously published papers (references 1 end 2), as thin-walled orthotropic spatial systems at the cross-sections of which only axial (normal and shearing) forces can arise. The longitudinal bending and twisting moments, due to their weak effect on the stress state of the shell, are taken equal to zero. Along the longitudinal sections of the shell there may arise transverse forces in addition to the normal d shearing forces. Under the so-called static assumptions there is taken for the computation model of the shell a thin-walled spatial system consisting along its length (along a generator) of an infinite number of elementary strips capable of bending. Each of these strips is likened to a curved rod operating in each of its sections not only in tension (compression)but also in transverse bending and shear. The interaction between two adjoining transverse strips in the shell expresses itself in the transmission from one strip to the other of only the normal and shearing stresses. The static structure of the computation model here described is shown in figure 1, where the connections through which the normal and shearing stresses transmitted from one transverse strip to smother are indicated schematically by the rods located in the middle surface of the shell. In addition to the static hypothesis we introduce also geometric hypotheses. According to the latter the elongational deformations of the shell along lines parallel to the generator of its middle surface and the shear deformations in the middle surface, as ma+gitudes having . little effect on the state of the fundamental internal forces of the shell, are taken equal to zero. The deformations of the shell in our computational model are such that in the first place the lines of this surface perpendicular to the generator are inextensible at each point end in the second place the angles between the lines of principal curvature (the coordinate lines) which are straight before the deformation remain straight after the deformation.

A Langevin dynamics simulation study of the tribology of polymer loop brushes.

PubMed

Yin, Fang; Bedrov, Dmitry; Smith, Grant D; Kilbey, S Michael

2007-08-28

The tribology of surfaces modified with doubly bound polymer chains (loops) has been investigated in good solvent conditions using Langevin dynamics simulations. The density profiles, brush interpenetration, chain inclination, normal forces, and shear forces for two flat substrates modified by doubly bound bead-necklace polymers and equivalent singly bound polymers (twice as many polymer chains of 12 the molecular weight of the loop chains) were determined and compared as a function of surface separation, grafting density, and shear velocity. The doubly bound polymer layers showed less interpenetration with decreasing separation than the equivalent singly bound layers. Surprisingly, this difference in interpenetration between doubly bound polymer and singly bound polymer did not result in decreased friction at high shear velocity possibly due to the decreased ability of the doubly bound chains to deform in response to the applied shear. However, at lower shear velocity, where deformation of the chains in the flow direction is less pronounced and the difference in interpenetration is greater between the doubly bound and singly bound chains, some reduction in friction was observed.

Shear Stress in Magnetorheological FInishing for Glasses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miao, C.; Shafrir, S.N.; Lambropoulos, J.C.

2009-04-28

We report in situ, simultaneous measurements of both drag and normal forces in magnetorheological finishing (MRF) for what is believed to be the first time, using a spot taking machine (STM) as a test bed to take MRF spots on stationary parts. The measurements are carried out over the entire area where material is being removed, i.e., the projected area of the MRF removal function/spot on the part surface, using a dual force sensor. This approach experimentally addresses the mechanisms governing material removal in MRF for optical glasses in terms of the hydrodynamic pressure and shear stress, applied by themore » hydrodynamic flow of magnetorheological fluid at the gap between the part surface and the STM wheel. This work demonstrates that the volumetric removal rate shows a positive linear dependence on shear stress. Shear stress exhibits a positive linear dependence on a material figure of merit that depends upon Young’s modulus, fracture toughness, and hardness. A modified Preston’s equation is proposed that better estimates MRF material removal rate for optical glasses by incorporating mechanical properties, shear stress, and velocity.« less

Shear stress in magnetorheological finishing for glasses.

PubMed

Miao, Chunlin; Shafrir, Shai N; Lambropoulos, John C; Mici, Joni; Jacobs, Stephen D

2009-05-01

We report in situ, simultaneous measurements of both drag and normal forces in magnetorheological finishing (MRF) for what is believed to be the first time, using a spot taking machine (STM) as a test bed to take MRF spots on stationary parts. The measurements are carried out over the entire area where material is being removed, i.e., the projected area of the MRF removal function/spot on the part surface, using a dual force sensor. This approach experimentally addresses the mechanisms governing material removal in MRF for optical glasses in terms of the hydrodynamic pressure and shear stress, applied by the hydrodynamic flow of magnetorheological fluid at the gap between the part surface and the STM wheel. This work demonstrates that the volumetric removal rate shows a positive linear dependence on shear stress. Shear stress exhibits a positive linear dependence on a material figure of merit that depends upon Young's modulus, fracture toughness, and hardness. A modified Preston's equation is proposed that better estimates MRF material removal rate for optical glasses by incorporating mechanical properties, shear stress, and velocity.

An Experimental Optical Three-axis Tactile Sensor Featured with Hemispherical Surface

NASA Astrophysics Data System (ADS)

Ohka, Masahiro; Kobayashi, Hiroaki; Takata, Jumpei; Mitsuya, Yasunaga

We are developing an optical three-axis tactile sensor capable of acquiring normal and shearing force to mount on a robotic finger. The tactile sensor is based on the principle of an optical waveguide-type tactile sensor, which is composed of an acrylic hemispherical dome, a light source, an array of rubber sensing elements, and a CCD camera. The sensing element of the silicone rubber comprises one columnar feeler and eight conical feelers. The contact areas of the conical feelers, which maintain contact with the acrylic dome, detect the three-axis force applied to the tip of the sensing element. Normal and shearing forces are then calculated from integration and centroid displacement of the grayscale value derived from the conical feeler's contacts. To evaluate the present tactile sensor, we conducted a series of experiments using an x-z stage, a rotational stage, and a force gauge. Although we discovered that the relationship between the integrated grayscale value and normal force depends on the sensor's latitude on the hemispherical surface, it is easy to modify the sensitivity based on the latitude to make the centroid displacement of the grayscale value proportional to the shearing force. When we examined the repeatability of the present tactile sensor with 1,000 load/unload cycles, the error was 2%.

Computational fluid dynamics simulation of transcatheter aortic valve degeneration.

PubMed

Dwyer, Harry A; Matthews, Peter B; Azadani, Ali; Jaussaud, Nicolas; Ge, Liang; Guy, T Sloane; Tseng, Elaine E

2009-08-01

Studied under clinical trials, transcatheter aortic valves (TAV) have demonstrated good short-term feasibility and results in high-risk surgical patients with severe aortic stenosis. However, their long-term safety and durability are unknown. The objective of this study is to evaluate hemodynamic changes within TAV created by bioprosthetic leaflet degeneration. Computational fluid dynamics (CFD) simulations were performed to evaluate the hemodynamics through TAV sclerosis (35% orifice reduction) and stenosis (78% orifice reduction). A three-dimensional surface mesh of the TAV within the aortic root was generated for each simulation. Leaflets were contained within an open, cylindrical body without attachment to the sinus commissures representing the stent. A continuous surface between the annulus and TAV excluded the geometry of the native calcified leaflets and prevented paravalvular leak. Unsteady control volume analysis throughout systole was used to calculate leaflet shear stress and total force on the TAV. Sclerosis increased total force on the TAV by 63% (0.602-0.98 N). Advancement of degeneration from sclerosis to stenosis was accompanied by an 86% increase in total force (1.82 N) but only a 32% increase in peak wall shear stress on the leaflets. Of the total force exerted on the TAV, 99% was in the direction of axial flow. Shear stresses on the TAV were greatest during peak systolic flow with stress concentrations on the tips of the leaflets. In the normal TAV, the aortic root geometry and physiologic flow dominate location and magnitude of shear stress. Following leaflet degeneration, the specific geometry of the stenosis dictates the profile of axial velocity leaving the TAV and shear stress on the leaflets. A dramatic increase in peak leaflet shear stress was observed (115 Pa stenosis vs. 87 Pa sclerosis and 29 Pa normal). CFD simulations in this study provide the first of its kind data quantifying hemodynamics within stenosed TAV. Stenosis leads to significant forces of TAV during systole; however, diastolic forces predominate even with significant stenosis. Substantial changes in peak shear stress occur with TAV degeneration. As the first implanted TAV begin to stenose, the authors recommend watchful examination for device failure.

Laminar shear stress modulates endothelial luminal surface stiffness in a tissue-specific manner.

PubMed

Merna, Nick; Wong, Andrew K; Barahona, Victor; Llanos, Pierre; Kunar, Balvir; Palikuqi, Brisa; Ginsberg, Michael; Rafii, Shahin; Rabbany, Sina Y

2018-04-17

Endothelial cells form vascular beds in all organs and are exposed to a range of mechanical forces that regulate cellular phenotype. We sought to determine the role of endothelial luminal surface stiffness in tissue-specific mechanotransduction of laminar shear stress in microvascular mouse cells and the role of arachidonic acid in mediating this response. Microvascular mouse endothelial cells were subjected to laminar shear stress at 4 dynes/cm 2 for 12 hours in parallel plate flow chambers that enabled real-time optical microscopy and atomic force microscopy measurements of cell stiffness. Lung endothelial cells aligned parallel to flow, while cardiac endothelial cells did not. This rapid alignment was accompanied by increased cell stiffness. The addition of arachidonic acid to cardiac endothelial cells increased alignment and stiffness in response to shear stress. Inhibition of arachidonic acid in lung endothelial cells and embryonic stem cell-derived endothelial cells prevented cellular alignment and decreased cell stiffness. Our findings suggest that increased endothelial luminal surface stiffness in microvascular cells may facilitate mechanotransduction and alignment in response to laminar shear stress. Furthermore, the arachidonic acid pathway may mediate this tissue-specific process. An improved understanding of this response will aid in the treatment of organ-specific vascular disease. © 2018 John Wiley & Sons Ltd.

Single Piezo-Actuator Rotary-Hammering Drill

NASA Technical Reports Server (NTRS)

Sherrit, Stewart; Bao, Xiaoqi; Badescu, Mircea; Bar-Cohen, Yoseph

2011-01-01

This innovation comprises a compact drill that uses low-axial preload, via vibrations, that fractures the rock under the bit kerf, and rotates the bit to remove the powdered cuttings while augmenting the rock fracture via shear forces. The vibrations fluidize the powered cuttings inside the flutes around the bit, reducing the friction with the auger surface. These combined actions reduce the consumed power and the heating of the drilled medium, helping to preserve the pristine content of the produced samples. The drill consists of an actuator that simultaneously impacts and rotates the bit by applying force and torque via a single piezoelectric stack actuator without the need for a gearbox or lever mechanism. This reduces the development/fabrication cost and complexity. The piezoelectric actuator impacts the surface and generates shear forces, fragmenting the drilled medium directly under the bit kerf by exceeding the tensile and/or shear strength of the struck surface. The percussive impact action of the actuator leads to penetration of the medium by producing a zone of finely crushed rock directly underneath the struck location. This fracturing process is highly enhanced by the shear forces from the rotation and twisting action. To remove the formed cuttings, the bit is constructed with an auger on its internal or external surface. One of the problems with pure hammering is that, as the teeth become embedded in the sample, the drilling efficiency drops unless the teeth are moved away from the specific footprint location. By rotating the teeth, they are moved to areas that were not fragmented, and thus the rock fracturing is enhanced via shear forces. The shear motion creates ripping or chiseling action to produce larger fragments to increase the drilling efficiency, and to reduce the required power. The actuator of the drill consists of a piezoelectric stack that vibrates the horn. The stack is compressed by a bolt between the backing and the horn in order to prevent it from being subjected to tensile stress that will cause it to fail. The backing is intended to transfer the generated mechanical vibrations towards the horn. In order to cause rotation, the horn is configured asymmetrically with helical segments and, upon impacting the bit, it introduces longitudinal along the axis of the actuator and tangential force causing twisting action that rotates the bit. The longitudinal component of the vibrations of the stack introduces percussion impulses between the bit and the rock to fracture it when the ultimate strain is exceeded under the bit.

Experimental study and FEM simulation of the simple shear test of cylindrical rods

NASA Astrophysics Data System (ADS)

Wirti, Pedro H. B.; Costa, André L. M.; Misiolek, Wojciech Z.; Valberg, Henry S.

2018-05-01

In the presented work an experimental simple shear device for cutting cylindrical rods was used to obtain force-displacement data for a low-carbon steel. In addition, and FEM 3D-simulation was applied to obtain internal shear stress and strain maps for this material. The experimental longitudinal grid patterns and force-displacement curve were compared with numerical simulation results. Many aspects of the elastic and plastic deformations were described. It was found that bending reduces the shear yield stress of the rod material. Shearing starts on top and bottom die-workpiece contact lines evolving in an arc-shaped area. Due to this geometry, stress concentrates on the surface of the rod until the level of damage reaches the critical value and the fracture starts here. The volume of material in the plastic zone subjected to shearing stress has a very complex shape and is function of a dimensionless geometrical parameter. Expressions to calculate the true shear stress τ and strain γ from the experimental force-displacement data were proposed. The equations' constants are determined by fitting the experimental curve with the stress τ and strain γ simulation point tracked data.

Application and optimization of the tenderization of pig Longissimus dorsi muscle by adenosine 5'-monophosphate (AMP) using the response surface methodology.

PubMed

Deng, Shaoying; Wang, Daoying; Zhang, Muhan; Geng, Zhiming; Sun, Chong; Bian, Huan; Xu, Weimin; Zhu, Yongzhi; Liu, Fang; Wu, Haihong

2016-03-01

Based on single factor experiments, NaCl concentration, adenosine 5'-monophosphate (AMP) concentration and temperature were selected as independent variables for a three-level Box-Behnken experimental design, and the shear force and cooking loss were response values for regression analysis. According to the statistical models, it showed that all independent variables had significant effects on shear force and cooking loss, and optimal values were at the NaCl concentration of 4.15%, AMP concentration of 22.27 mmol/L and temperature of 16.70°C, which was determined with three-dimensional response surface diagrams and contour plots. Under this condition, the observed shear force and cooking loss were 0.625 kg and 8.07%, respectively, exhibiting a good agreement with their predicted values, showing the good applicability and feasibility of response surface methodology (RSM) for improving pork tenderness. Compared with control pig muscles, AMP combined with NaCl treatment demonstrated significant effects on improvement of meat tenderness and reduction of cooking loss. Therefore, AMP could be regarded as an effective tenderization agent for pork. © 2015 Japanese Society of Animal Science.

Influence of Brownian motion on blood platelet flow behavior and adhesive dynamics near a planar wall.

PubMed

Mody, Nipa A; King, Michael R

2007-05-22

We used the platelet adhesive dynamics computational method to study the influence of Brownian motion of a platelet on its flow characteristics near a surface in the creeping flow regime. Two important characterizations were done in this regard: (1) quantification of the platelet's ability to contact the surface by virtue of the Brownian forces and torques acting on it, and (2) determination of the relative importance of Brownian motion in promoting surface encounters in the presence of shear flow. We determined the Peclet number for a platelet undergoing Brownian motion in shear flow, which could be expressed as a simple linear function of height of the platelet centroid, H from the surface Pe (platelet) = . (1.56H + 0.66) for H > 0.3 microm. Our results demonstrate that at timescales relevant to shear flow in blood Brownian motion plays an insignificant role in influencing platelet motion or creating further opportunities for platelet-surface contact. The platelet Peclet number at shear rates >100 s-1 is large enough (>200) to neglect platelet Brownian motion in computational modeling of flow in arteries and arterioles for most practical purposes even at very close distances from the surface. We also conducted adhesive dynamics simulations to determine the effects of platelet Brownian motion on GPIbalpha-vWF-A1 single-bond dissociation dynamics. Brownian motion was found to have little effect on bond lifetime and caused minimal bond stressing as bond rupture forces were calculated to be less than 0.005 pN. We conclude from our results that, for the case of platelet-shaped cells, Brownian motion is not expected to play an important role in influencing flow characteristics, platelet-surface contact frequency, and dissociative binding phenomena under flow at physiological shear rates (>50 s(-1)).

Development of Wearable Sheet-Type Shear Force Sensor and Measurement System that is Insusceptible to Temperature and Pressure.

PubMed

Toyama, Shigeru; Tanaka, Yasuhiro; Shirogane, Satoshi; Nakamura, Takashi; Umino, Tokio; Uehara, Ryo; Okamoto, Takuma; Igarashi, Hiroshi

2017-07-31

A sheet-type shear force sensor and a measurement system for the sensor were developed. The sensor has an original structure where a liquid electrolyte is filled in a space composed of two electrode-patterned polymer films and an elastic rubber ring. When a shear force is applied on the surface of the sensor, the two electrode-patterned films mutually move so that the distance between the internal electrodes of the sensor changes, resulting in current increase or decrease between the electrodes. Therefore, the shear force can be calculated by monitoring the current between the electrodes. Moreover, it is possible to measure two-dimensional shear force given that the sensor has multiple electrodes. The diameter and thickness of the sensor head were 10 mm and 0.7 mm, respectively. Additionally, we also developed a measurement system that drives the sensor, corrects the baseline of the raw sensor output, displays data, and stores data as a computer file. Though the raw sensor output was considerably affected by the surrounding temperature, the influence of temperature was drastically decreased by introducing a simple arithmetical calculation. Moreover, the influence of pressure simultaneously decreased after the same calculation process. A demonstrative measurement using the sensor revealed the practical usefulness for on-site monitoring.

Contribution of Surface Chemistry to the Shear Thickening of Silica Nanoparticle Suspensions.

PubMed

Yang, Wufang; Wu, Yang; Pei, Xiaowei; Zhou, Feng; Xue, Qunji

2017-01-31

Shear thickening is a general process crucial for many processed products ranging from food and personal care to pharmaceuticals. Theoretical calculations and mathematical simulations of hydrodynamic interactions and granular-like contacts have proved that contact forces between suspended particles dominate the rheological characteristic of colloidal suspensions. However, relevant experimental studies are very rare. This study was conducted to reveal the influence of nanoparticle (NP) interactions on the rheological behavior of shear-thickening fluids (STFs) by changing the colloidal surface chemistries. Silica NPs with various surface chemical compositions are fabricated and used to prepare dense suspensions. Rheological experiments are conducted to determine the influence of NP interactions on corresponding dense suspension systems. The results suggest that the surface chemistries of silica NPs determine the rheological behavior of dense suspensions, including shear-thickening behavior, onset stress, critical volume fraction, and jamming volume fraction. This study provides useful reference for designing effective STFs and regulating their characteristics.

Simulating Regoliths in a Microgravity Environment

NASA Astrophysics Data System (ADS)

Murdoch, N.; Rozitis, B.; Green, S. F.; Michel, P.; Losert, W.; de Lophem, T. L.

2011-10-01

The dynamics of granular materials are involved in the evolution of solid planets and small bodies in our Solar System, whose surfaces are generally covered with regolith. An understanding of granular dynamics appears also to be critical for the design and/or operations of landers, sampling devices and rovers to be included in space missions. The AstEx experiment uses a microgravity modified Taylor-Couette shear cell to investigate granular motion caused by shear and shear reversal forces under the microgravity conditions of parabolic flight. The results will lead to a greater understanding of the mechanical response of granular materials subject to external forces in varying gravitational environments.

A Two-Axis Direct Fluid Shear Stress Sensor

NASA Technical Reports Server (NTRS)

Adcock, Edward E.; Scott, Michael A.; Bajikar, Sateesh S.

2010-01-01

This innovation is a miniature or micro sized semiconductor sensor design that provides two axis direct non-intrusive measurement of skin friction or wall shear stress in fluid flow. The sensor is fabricated by micro-electro-mechanical system (MEMS) technology, enabling small size and low cost reproductions. The sensors have been fabricated by utilizing MEMS fabrication processes to bond a sensing element wafer to a fluid coupling wafer. This layering technique provides for an out of plane dimension that is on the same order of length as the inplane dimensions. The sensor design has the following characteristics: a shear force collecting plate with dimensions that can be tailored to various application specific requirements such as spatial resolution, temporal resolution and shear force range and resolution. This plate is located coplanar to both the sensor body and flow boundary, and is connected to a dual axis gimbal structure by a connecting column or lever arm. The dual axis gimbal structure has torsional hinges with embedded piezoresistive torsional strain gauges which provide a voltage output that is correlated to the applied shear stress (and excitation current) on force collection plate that is located on the flow boundary surface (hence the transduction method). This combination of design elements create a force concentration and resolution structure that enables the generation of a large stress on the strain gauge from the small shear stress on the flow boundary wall. This design as well as the use of back side electrical contacts establishes a non-intrusive method to quantitatively measure the shear force vector on aerodynamic bodies.

High glucose attenuates shear-induced changes in endothelial hydraulic conductivity by degrading the glycocalyx.

PubMed

Lopez-Quintero, Sandra V; Cancel, Limary M; Pierides, Alexis; Antonetti, David; Spray, David C; Tarbell, John M

2013-01-01

Diabetes mellitus is a risk factor for cardiovascular disease; however, the mechanisms through which diabetes impairs homeostasis of the vasculature have not been completely elucidated. The endothelium interacts with circulating blood through the surface glycocalyx layer, which serves as a mechanosensor/transducer of fluid shear forces leading to biomolecular responses. Atherosclerosis localizes typically in regions of low or disturbed shear stress, but in diabetics, the distribution is more diffuse, suggesting that there is a fundamental difference in the way cells sense shear forces. In the present study, we examined the effect of hyperglycemia on mechanotranduction in bovine aortic endothelial cells (BAEC). After six days in high glucose media, we observed a decrease in heparan sulfate content coincident with a significant attenuation of the shear-induced hydraulic conductivity response, lower activation of eNOS after exposure to shear, and reduced cell alignment with shear stress. These studies are consistent with a diabetes-induced change to the glycocalyx altering endothelial response to shear stress that could affect the distribution of atherosclerotic plaques.

High Glucose Attenuates Shear-Induced Changes in Endothelial Hydraulic Conductivity by Degrading the Glycocalyx

PubMed Central

Lopez-Quintero, Sandra V.; Cancel, Limary M.; Pierides, Alexis; Antonetti, David; Spray, David C.; Tarbell, John M.

2013-01-01

Diabetes mellitus is a risk factor for cardiovascular disease; however, the mechanisms through which diabetes impairs homeostasis of the vasculature have not been completely elucidated. The endothelium interacts with circulating blood through the surface glycocalyx layer, which serves as a mechanosensor/transducer of fluid shear forces leading to biomolecular responses. Atherosclerosis localizes typically in regions of low or disturbed shear stress, but in diabetics, the distribution is more diffuse, suggesting that there is a fundamental difference in the way cells sense shear forces. In the present study, we examined the effect of hyperglycemia on mechanotranduction in bovine aortic endothelial cells (BAEC). After six days in high glucose media, we observed a decrease in heparan sulfate content coincident with a significant attenuation of the shear-induced hydraulic conductivity response, lower activation of eNOS after exposure to shear, and reduced cell alignment with shear stress. These studies are consistent with a diabetes-induced change to the glycocalyx altering endothelial response to shear stress that could affect the distribution of atherosclerotic plaques. PMID:24260138

The Effect of Two Soft Drinks on Bracket Bond Strength and on Intact and Sealed Enamel: An In Vitro Study.

PubMed

Pasha, Azam; Sindhu, D; Nayak, Rabindra S; Mamatha, J; Chaitra, K R; Vishwakarma, Swati

2015-01-01

This study was conducted to evaluate the effect of two soft drinks, Coca-Cola and Mirinda orange on bracket bond strength, on adhesive remnant on teeth after debonding the bracket, and to observe by means of scanning electron microscope (SEM) the effect of these drinks on intact and sealed enamel. 120 non-carious maxillary premolar teeth already extracted for Orthodontic purposes were taken and divided into three groups, i.e., Coca-Cola drink, Mirinda orange, and control (artificial saliva) group. Brackets were bonded using conventional methods. Teeth were kept in soft drinks for 15 days, for 15 min, 3 times a day, separated by intervals of 2 h. At other times, they were kept in artificial saliva. The samples, thus obtained were evaluated for shear bond strength using the universal testing machine and subsequently subjected for adhesive remnant index (ARI) scores. SEM study on all the three groups was done for evaluating enamel surface of the intact and sealed enamel. The lowest mean resistance to shearing forces was shown by Mirinda orange group (5.30 ± 2.74 Mpa) followed by Coca-Cola group (6.24 ± 1.59 Mpa) and highest resistance to shearing forces by control group (7.33 ± 1.72 Mpa). The ARI scores revealed a cohesive failure in control samples and an adhesive failure in Mirinda and cola samples. SEM results showed areas of defect due to erosion caused by acidic soft drinks on intact and sealed enamel surface. Mirinda group showed the lowest resistance to shearing forces, followed by Coca-Cola group and with the highest resistance to shearing forces by the control group. There were significant differences between the control group and the study groups. Areas of defects, which were caused by erosion related to acidic soft drinks on the enamel surface around the adhesive, were seen. Areas of defects caused by Coca-Cola were more extensive when compared to Mirinda orange drink.

Smoothing and roughening of slip surfaces in direct shear experiments

NASA Astrophysics Data System (ADS)

Sagy, Amir; Badt, Nir; Hatzor, Yossef H.

2015-04-01

Faults in the upper crust contain discrete slip surfaces which have absorbed a significant part of the shear displacement along them. Field measurements demonstrate that these surfaces are rough at all measurable scales and indicate that surfaces of relatively large-slip faults are statistically smoother than those of small-slip faults. However, post faulting and surface erosion process that might affect the geometry of outcrops cannot be discounted in such measurements. Here we present experimental results for the evolution of shear surface topography as function of slip distance and normal stress in direct shear experiments. A single prismatic fine grain limestone block is first fractured in tension mode using the four-point bending test methodology and then the fracture surface topography is scanned using a laser profilometer. We then shear the obtained tensile fracture surfaces in direct shear, ensuring the original fracture surfaces are in a perfectly matching configuration at the beginning of the shear test. First, shearing is conducted to distances varying from 5 to 15 mm under constant normal stress of 2MPa and a constant displacement rate of 0.05 mm/s using two closed-loop servo controlled hydraulic pistons, supplying normal and shear forces (Davidesko et al., 2014). In the tested configuration peak shear stress is typically attained after a shear displacement of about 2-3 mm, beyond which lower shear stress is required to continue shearing at the preset displacement rate of 0.05 mm/s as is typical for initially rough joints. Following some initial compression the interface begins to dilate and continues to do so until the end of the test. The sheared tensile fracture surface is then scanned again and the geometrical evolution, in term of RMS roughness and power spectral density (PSD) is analyzed. We show that shearing smooth the surface along all our measurements scales. The roughness ratio, measured by initial PSD / final PSD for each wavelength, increases as a function of slip amount. The roughness measured after slip can be fitted by a power-law similar to that of the initial tensile surface. In the next series of experiments a similar procedure is applied when the roughness evolution is measured as a function of increasing normal stress for a fixed displacement amount of 10 mm. While samples sheared under a constant normal stress of 5 MPa generated surface smoothing, shearing under normal stress of 7.5 MPa to 15 MPa exhibited surface roughening at the measured range of scales. We find that roughening is correlated with the attained peak shear stress values, stress drop (peak shear stress minus residual shear stress) and with wear accumulation, a novel measurement procedure of which is developed here. Analysis of the sheared samples shows that roughening is generated by sets of dense fractures that significantly damaged the sample in the immediate proximity to large asperities. This roughening is related to penetrative damage during transient wear in rough surfaces.

The effects of non-Newtonian viscosity on the deformation of red blood cells in a shear flow

NASA Astrophysics Data System (ADS)

Sesay, Juldeh

2005-11-01

The analyses of the effects of non-Newtonian viscosity on the membrane of red blood cells (RBCs) suspended in a shear flow are presented. The specific objective is to investigate the mechanical deformation on the surfaces of an ellipsoidal particle model. The hydrodynamic stresses and other forces on the surface of the particle are used to determine the cell deformation. We extended previous works, which were based on the Newtonian fluid models, to the non-Newtonian case, and focus on imposed shear rate values between 1 and 100 per second. Two viscosity models are investigated, which respectively correspond to a normal person and a patient with cerebrovascular accident (CVA). The results are compared with those obtained assuming a Newtonian model. We observed that the orientation of the cell influences the deformation and the imposed shear rate drives the local shear rate distribution along the particle surface. The integral particle deformation for the non-Newtonian models in the given shear rate regime is higher than that for the Newtonian reference model. Finally, the deformation of the cell surface decreases as the dissipation ratio increases.

Load estimation from photoelastic fringe patterns under combined normal and shear forces

NASA Astrophysics Data System (ADS)

Dubey, V. N.; Grewal, G. S.

2009-08-01

Recently there has been some spurt of interests to use photoelastic materials for sensing applications. This has been successfully applied for designing a number of signal-based sensors, however, there have been limited efforts to design image-based sensors on photoelasticity which can have wider applications in term of actual loading and visualisation. The main difficulty in achieving this is the infinite loading conditions that may generate same image on the material surface. This, however, can be useful for known loading situations as this can provide dynamic and actual conditions of loading in real time. This is particularly useful for separating components of forces in and out of the loading plane. One such application is the separation of normal and shear forces acting on the plantar surface of foot of diabetic patients for predicting ulceration. In our earlier work we have used neural networks to extract normal force information from the fringe patterns using image intensity. This paper considers geometric and various other statistical parameters in addition to the image intensity to extract normal as well as shear force information from the fringe pattern in a controlled experimental environment. The results of neural network output with the above parameters and their combinations are compared and discussed. The aim is to generalise the technique for a range of loading conditions that can be exploited for whole-field load visualisation and sensing applications in biomedical field.

Nd:YAG Laser-aided ceramic brackets debonding: Effects on shear bond strength and enamel surface

NASA Astrophysics Data System (ADS)

Han, Xianglong; Liu, Xiaolin; Bai, Ding; Meng, Yao; Huang, Lan

2008-11-01

In order to evaluate the efficiency of Nd:YAG laser-aided ceramic brackets debonding technique, both ceramic brackets and metallic brackets were bonded with orthodontic adhesive to 30 freshly extracted premolars. The specimens were divided into three groups, 10 in each, according to the brackets employed and the debonding techniques used: (1) metallic brackets with shear debonding force, (2) ceramic brackets with shear debonding force, and (3) ceramic brackets with Nd:YAG laser irradiation. The result showed that laser irradiation could diminish shear bond strength (SBS) significantly and produce the most desired ARI scores. Moreover, scanning electron microscopy investigation displayed that laser-aided technique induced little enamel scratch or loss. It was concluded that Nd:YAG laser could facilitate the debonding of ceramic brackets and diminish the amount of remnant adhesive without damaging enamel structure.

Effect of surface roughness of hydroxyapatite-coated titanium on the bone-implant interface shear strength.

PubMed

Hayashi, K; Inadome, T; Tsumura, H; Nakashima, Y; Sugioka, Y

1994-11-01

We have investigated the bone-implant interface shear strength of hydroxyapatite (HA)-coated Ti-6Al-4V (HA-coating A) (roughness average, Ra = 3.4 +/- 0.5 microns) and HA-coated Ti-6Al-4V with a rougher surface (HA-coating B) (Ra = 8.4 +/- 1.8 microns). There was no significant difference between HA-coating A and HA-coating B implants with respect to the bone-implant interface shear strength as determined in push-out tests using the transcortical model in adult dogs. The bone-implant interface shear strength of bead-coated porous Ti-6Al-4V was significantly greater than that of both HA-coating A and HA-coating B implants. The failure site, as determined by scanning electron microscopy, was the coating-substrate interface, not the coating-bone interface. This indicates a need to protect the HA coating from the direct shear forces. HA coating enhances early bone growth into the porous surface of the implant. Long-term fixation should depend on bone anchoring to this porous surface. Hydroxyapatite coatings must be developed which do not obstruct the pores of the surface of the implant.

Force fluctuations while pressing and moving against high- and low-friction touch screen surfaces.

PubMed

Joshi, Mukta N; Keenan, Kevin G

2016-07-01

The purpose of this study was to identify the influence of a high- and low-friction surface on the ability to maintain a steady downward force during an index finger pressing and moving task. Fifteen right-handed subjects (24-48 years) performed a static force pressing task and a hybrid pressing and moving task on the surface of an iPad mini while holding a steady 2-N force on high- and low-friction surfaces. Variability of force was quantified as the standard deviation (SD) of normal force (F z) and shear force (F xy) across friction conditions and tasks. The SD of F z was 227 % greater during the hybrid task as compared to the static task (p < .001) and was 19 % greater for the high- versus low-friction condition (p = .033). There were positive correlations between SD of F z and F xy during the hybrid force/motion tasks on the high- and low-friction conditions (r (2) = 0.5 and 0.86, respectively), suggesting significant associations between normal and shear forces for this hybrid task. The correlation between the SD of F z for static and hybrid tasks was r (2) = 0.44, indicating that the common practice of examining the control of static tasks may not sufficiently explain performance during hybrid tasks, at least for the young subjects tested in the current study. As activities of daily living frequently require hybrid force/motion tasks (e.g., writing, doing the dishes, and cleaning counters), the results of this study emphasize the need to study motor performance during hybrid tasks in addition to static force tasks.

Relationship between enamel bond fatigue durability and surface free-energy characteristics with universal adhesives.

PubMed

Nagura, Yuko; Tsujimoto, Akimasa; Barkmeier, Wayne W; Watanabe, Hidehiko; Johnson, William W; Takamizawa, Toshiki; Latta, Mark A; Miyazaki, Masashi

2018-04-01

The relationship between enamel bond fatigue durability and surface free-energy characteristics with universal adhesives was investigated. The initial shear bond strengths and shear fatigue strengths of five universal adhesives to enamel were determined with and without phosphoric acid pre-etching. The surface free-energy characteristics of adhesive-treated enamel with and without pre-etching were also determined. The initial shear bond strength and shear fatigue strength of universal adhesive to pre-etched enamel were higher than those to ground enamel. The initial shear bond strength and shear fatigue strength of universal adhesive to pre-etched enamel were material dependent, unlike those to ground enamel. The surface free-energy of the solid (γ S ) and the hydrogen-bonding force (γSh) of universal adhesive-treated enamel were different depending on the adhesive, regardless of the presence or absence of pre-etching. The bond fatigue durability of universal adhesives was higher to pre-etched enamel than to ground enamel. In addition, the bond fatigue durability to pre-etched enamel was material dependent, unlike that to ground enamel. The surface free-energy characteristics of universal adhesive-treated enamel were influenced by the adhesive type, regardless of the presence or absence of pre-etching. The surface free-energy characteristics of universal adhesive-treated enamel were related to the results of the bond fatigue durability. © 2018 Eur J Oral Sci.

Nanomachining by rubbing at ultrasonic frequency under controlled shear force.

PubMed

Muraoka, Mikio

2011-03-01

This study proposes a new method of proximal-probe machining that uses a rubbing process by introducing concentrated-mass (CM) cantilevers. At the second resonance of the CM cantilever vibration, the tip site of the cantilever becomes a node of the standing deflection wave because of the sufficient inertia of the attached concentrated mass. The tip makes a cyclic motion that is tangential to the sample surface, not vertical to it, as in a tapping motion. This lateral tip motion that is selectively excited by CM cantilevers was effective for the material modification of a sample due to the friction between the tip and the sample. Imaging and nanomachining under controlled shear force were demonstrated by means of the modified cantilever and a normal atomic force microscope. We were able to write a micron-sized letter "Z" having a line width of 30-100 nm on a resin surface.

Idealized Cloud-System Resolving Modeling for Tropical Convection Studies

NASA Astrophysics Data System (ADS)

Anber, Usama M.

A three-dimensional limited-domain Cloud-Resolving Model (CRM) is used in idealized settings to study the interaction between tropical convection and the large scale dynamics. The model domain is doubly periodic and the large-scale circulation is parameterized using the Weak Temperature Gradient (WTG) Approximation and Damped Gravity Wave (DGW) methods. The model simulations fall into two main categories: simulations with a prescribed radiative cooling profile, and others in which radiative cooling profile interacts with clouds and water vapor. For experiments with a prescribed radiative cooling profile, radiative heating is taken constant in the vertical in the troposphere. First, the effect of turbulent surface fluxes and radiative cooling on tropical deep convection is studied. In the precipitating equilibria, an increment in surface fluxes produces a greater increase in precipitation than an equal increment in column-integrated radiative heating. The gross moist stability remains close to constant over a wide range of forcings. With dry initial conditions, the system exhibits hysteresis, and maintains a dry state with for a wide range of net energy inputs to the atmospheric column under WTG. However, for the same forcings the system admits a rainy state when initialized with moist conditions, and thus multiple equilibria exist under WTG. When the net forcing is increased enough that simulations, which begin dry, eventually develop precipitation. DGW, on the other hand, does not have the tendency to develop multiple equilibria under the same conditions. The effect of vertical wind shear on tropical deep convection is also studied. The strength and depth of the shear layer are varied as control parameters. Surface fluxes are prescribed. For weak wind shear, time-averaged rainfall decreases with shear and convection remains disorganized. For larger wind shear, rainfall increases with shear, as convection becomes organized into linear mesoscale systems. This non-monotonic dependence of rainfall on shear is observed when the imposed surface fluxes are moderate. For larger surface fluxes, convection in the unsheared basic state is already strongly organized, but increasing wind shear still leads to increasing rainfall. In addition to surface rainfall, the impacts of shear on the parameterized large-scale vertical velocity, convective mass fluxes, cloud fraction, and momentum transport are also discussed. For experiments with interactive radiative cooling profile, the effect of cloud-radiation interaction on cumulus ensemble is examined in sheared and unsheared environments with both fixed and interactive sea surface temperature (SST). For fixed SST, interactive radiation, when compared to simulations in which radiative profile has the same magnitude and vertical shape but does not interact with clouds or water vapor, is found to suppress mean precipitation by inducing strong descent in the lower troposphere, increasing the gross moist stability. For interactive SST, using a slab ocean mixed layer, there exists a shear strength above which the system becomes unstable and develops oscillatory behavior. Oscillations have periods of wet precipitating states followed by periods of dry non-precipitating states. The frequencies of oscillations are intraseasonal to subseasonal, depending on the mixed layer depth. Finally, the model is coupled to a land surface model with fully interactive radiation and surface fluxes to study the diurnal and seasonal radiation and water cycles in the Amazon basin. The model successfully captures the afternoon precipitation and cloud cover peak and the greater latent heat flux in the dry season for the first time; two major biases in GCMs with implications for correct estimates of evaporation and gross primary production in the Amazon. One of the key findings is that the fog layer near the surface in the west season is crucial for determining the surface energy budget and precipitation. This suggests that features on the diurnal time scale can significantly impact climate on the seasonal time scale.

In-situ atomic force microscopy observation revealing gel-like plasticity on a metallic glass surface

NASA Astrophysics Data System (ADS)

Lu, Y. M.; Zeng, J. F.; Huang, J. C.; Kuan, S. Y.; Nieh, T. G.; Wang, W. H.; Pan, M. X.; Liu, C. T.; Yang, Y.

2017-03-01

It has been decade-long and enduring efforts to decipher the structural mechanism of plasticity in metallic glasses; however, it still remains a challenge to directly reveal the structural change, if any, that precedes; and dominant plastics flow in them. Here, by using the dynamic atomic force microscope as an "imaging" as well as a "forcing" tool, we unfold a real-time sequence of structural evolution occurring on the surface of an Au-Si thin film metallic glass. In sharp contrast to the common notion that plasticity comes along with mechanical softening in bulk metallic glasses, our experimental results directly reveal three types of nano-sized surface regions, which undergo plasticity but exhibit different characters of structural evolution following the local plasticity events, including stochastic structural rearrangement, unusual local relaxation and rejuvenation. As such, yielding on the metallic-glass surface manifests as a dynamic equilibrium between local relaxation and rejuvenation as opposed to shear instability in bulk metallic-glasses. Our finding demonstrates that plasticity on the metallic glass surface of Au-Si metallic glass bears much resemblance to that of the colloidal gels, of which nonlinear rheology rather than shear instability governs the constitutive behavior of plasticity.

Influence of Brownian Motion on Blood Platelet Flow Behavior and Adhesive Dynamics near a Planar Wall

PubMed Central

Mody, Nipa A.; King, Michael R.

2008-01-01

We used the Platelet Adhesive Dynamics computational method to study the influence of Brownian motion of a platelet on its flow characteristics near a surface in the creeping flow regime. Two important characterizations were done in this regard: (1) quantification of the platelet’s ability to contact the surface by virtue of the Brownian forces and torques acting on it, and (2) determination of the relative importance of Brownian motion in promoting surface encounters in the presence of shear flow. We determined the Peclet number for a platelet undergoing Brownian motion in shear flow, which could be expressed as a simple linear function of height of the platelet centroid, H from the surface Pe (platelet) = γ. · (1.56H + 0.66) for H > 0.3 μm. Our results demonstrate that at timescales relevant to shear flow in blood, Brownian motion plays an insignificant role in influencing platelet motion or creating further opportunities for platelet-surface contact. The platelet Peclet number at shear rates > 100 s-1 is large enough (> 200) to neglect platelet Brownian motion in computational modeling of flow in arteries and arterioles for most practical purposes even at very close distances from the surface. We also conducted adhesive dynamics simulations to determine the effects of platelet Brownian motion on GPIbα-vWF-A1 single-bond dissociation dynamics. Brownian motion was found to have little effect on bond lifetime and caused minimal bond stressing as bond rupture forces were calculated to be less than 0.005 pN. We conclude from our results that for the case of platelet-shaped cells, Brownian motion is not expected to play an important role in influencing flow characteristics, platelet-surface contact frequency and dissociative binding phenomena under flow at physiological shear rates (> 50 s-1). PMID:17417890

Evaluation of adhesion force between functionalized microbeads and protein-coated stainless steel using shear-flow-induced detachment.

PubMed

Mercier-Bonin, Muriel; Adoue, Mathieu; Zanna, Sandrine; Marcus, Philippe; Combes, Didier; Schmitz, Philippe

2009-10-01

Spherical microbeads functionalized with two types of chemical groups (NH(2), OH) were chosen as a simplified bacterial model, in order to elucidate the role of macromolecular interactions between specific biopolymers and 316 L stainless steel, in the frame of biofilm formation in the marine environment. NH(2) microbeads were used in their native form or after covalent binding to BSA or different representative poly-amino acids. OH microbeads were used in their native form. Adhesion force between microbeads and bare or BSA-coated stainless steel was quantified at nanoscale. Shear-flow-induced detachment experiments were combined with a simplified version of a theoretical model, based on the balance of hydrodynamic forces and torque exerted on microbeads. A maximal adhesion force of 27.6+/-8.5 nN was obtained for BSA-coated NH(2) microbeads. The high reactivity of OH functional groups was assessed (adhesion force of 15.6+/-4.8 nN for large microbeads). When charge-conducting stainless steel was coated with BSA, adhesion force was significantly lower than the one estimated with the bare surface, probably due to an increase in hydrophilic surface properties or suppression of charge transfer. The mechanism for microbead detachment was established (mainly rolling). The flow chamber and the associated theoretical modelling were demonstrated to be a relevant approach to quantify nanoscale forces between interacting surfaces.

Microgravity Effects on Transendothelial Transport

NASA Technical Reports Server (NTRS)

Tarbell, John M.

1996-01-01

The Endothelial Cell (EC) layer which lines blood vessels from the aorta to the capillaries provides the principal barrier to transport of water and solutes between blood and underlying tissue. Endothelial cells are continuously exposed to the mechanical shearing force (shear stress) and normal force (pressure) imposed by flowing blood on their surface, and they are adapted to this mechanical environment. When the cardiovascular system is exposed to microgravity, the mechanical environmental of endothelial cells is perturbed drastically and the transport properties of EC layers are altered in response. We have shown recently that step changes in shear stress have an acute effect on transport properties of EC layers in a cell culture model, and several recent studies in different vessels of live animals have confirmed the shear-dependent transport properties of the endothelium. We hypothesize that alterations in mechanical forces induced by microgravity and their resultant influence on transendothelial transport of water and solutes are, in large measure, responsible for the characteristic cephalad fluid shift observed in humans experiencing microgravity. To study the effects of altered mechanical forces on transendothelial transport and to test pharmacologic agents as counter measures to microgravity induced fluid shifts we have proposed ground-based studies using well defined cell culture models.

Quantifying the effects of external shear loads on arterial and venous blood flow: implications for pressure ulcer development.

PubMed

Manorama, Abinand; Meyer, Ronald; Wiseman, Robert; Bush, Tamara Reid

2013-06-01

Forces applied to the skin cause a decrease in regional blood flow. This decrease in blood flow can cause tissue necrosis and lead to the formation of deep, penetrating wounds called pressure ulcers. These wounds are detrimental to individuals with compromised health, such as the elderly and spinal-cord injured. Although surface pressure is known to be a primary risk factor for developing a pressure ulcer, a seated individual rarely experiences pressure alone but rather combined loading which includes pressure as well as shear force on the skin. However, little research has been conducted to quantify the effects of shear forces on blood flow. Fifteen men were tested in a magnetic resonance imaging scanner under no load, a normal load, and a combination of normal and shear loads. Changes in arterial and venous blood flow in the forearm were measured using magnetic resonance angiography phase-contrast imaging. The blood flow in the anterior interosseous artery and basilic vein of the forearm decreased with the application of normal loads, and decreased further with the addition of shear loads. Marginal to significant differences at a 90% confidence level (P=0.08, 0.10) were observed, and medium to high effect sizes (0.3 to 0.5) were obtained. Based on these results, shear force is an important factor to consider in relation to pressure ulcer propagation and prevention, and hence, future prevention approaches should also focus on mitigating shear loads. Copyright © 2013 Elsevier Ltd. All rights reserved.

Probing the Mechanical Properties of Plasma von Willebrand Factor Using Atomic Force Microscopy

NASA Astrophysics Data System (ADS)

Wijeratne, Sitara; Botello, Eric; Frey, Eric; Kiang, Ching-Hwa; Dong, Jing-Fei; Yeh, Hui-Chun

2010-03-01

Single-molecule manipulation allows us to study the real time kinetics of many complex cellular processes. The mechanochemistry of different forms of von Willebrand factor (VWF) and their receptor-ligand binding kinetics can be unraveled by atomic force microscopy (AFM). Since plasma VWF can be activated upon shear, the structural and functional properties of VWF are critical in mediating thrombus formation become important. Here we characterized the mechanical resistance to domain unfolding of VWF to determine the conformational states of VWF. We found the shear induced conformational, hence mechanical property changes can be detected by the change in unfolding forces. The relaxation rate of such effect is much longed than expected. This supports the model of lateral association VWF under shear stress. Our results offer an insight in establishing strategies for regulating VWF adhesion activity, increasing our understanding of surface-induced thrombosis as mediated by VWF.

Spontaneous Blinking from a Tribological Viewpoint.

PubMed

Pult, Heiko; Tosatti, Samuele G P; Spencer, Nicholas D; Asfour, Jean-Michel; Ebenhoch, Michael; Murphy, Paul J

2015-07-01

The mechanical forces between the lid wiper and the ocular surface, and between a contact lens and the lid wiper, are reported to be related to dry eye symptoms. Furthermore, the mechanical forces between these sliding partners are assumed to be related to the ocular signs of lid-wiper epitheliopathy (LWE) and lid-parallel conjunctival folds (LIPCOF). Recent literature provides some evidence that a contact lens with a low coefficient of friction (CoF) improves wearing comfort by reducing the mechanical forces between the contact lens surface and the lid wiper. This review discusses the mechanical forces during spontaneous blinks from a tribological perspective, at both low and high sliding velocities, in a healthy subject. It concludes that the coefficient of friction of the ocular surfaces appears to be strongly comparable to that of hydrophilic polymer brushes at low sliding velocity, and that, with increased sliding velocity, there is no wear at the sliding partners' surfaces thanks to the presence of a fluid film between the two sliding partners. In contrast, in the case of dry eye, the failure to maintain a full fluid film lubrication regime at high blinking speeds may lead to increased shear rates, resulting in deformation and wear of the sliding pairs. These shear rates are most likely related to tear film viscosity. Copyright © 2015 Elsevier Inc. All rights reserved.

Why do naked singularities form in gravitational collapse? II

DOE Office of Scientific and Technical Information (OSTI.GOV)

Joshi, Pankaj S.; Goswami, Rituparno; Dadhich, Naresh

We examine physical features that could lead to formation of a naked singularity rather than black hole, as end state of spherical collapse. Generalizing earlier results on dust collapse to general type I matter fields, it is shown that collapse always creates black hole if shear vanishes or density is homogeneous. It follows that nonzero shear is a necessary condition for singularity to be visible to external observers, when trapped surface formation is delayed by shearing forces or inhomogeneity within the collapsing cloud.

Surface deformation and shear flow in ligand mediated cell adhesion.

PubMed

Sircar, Sarthok; Roberts, Anthony J

2016-10-01

We present a unified, multiscale model to study the attachment/detachment dynamics of two deforming, charged, near spherical cells, coated with binding ligands and subject to a slow, homogeneous shear flow in a viscous, ionic fluid medium. The binding ligands on the surface of the cells experience both attractive and repulsive forces in an ionic medium and exhibit finite resistance to rotation via bond tilting. The microscale drag forces and couples describing the fluid flow inside the small separation gap between the cells, are calculated using a combination of methods in lubrication theory and previously published numerical results. For a selected range of material and fluid parameters, a hysteretic transition of the sticking probability curves (i.e., the function [Formula: see text]) between the adhesion phase (when [Formula: see text]) and the fragmentation phase (when [Formula: see text]) is attributed to a nonlinear relation between the total nanoscale binding forces and the separation gap between the cells. We show that adhesion is favoured in highly ionic fluids, increased deformability of the cells, elastic binders and a higher fluid shear rate (until a critical threshold value of shear rate is reached). Within a selected range of critical shear rates, the continuation of the limit points (i.e., the turning points where the slope of [Formula: see text] changes sign) predict a bistable region, indicating an abrupt switching between the adhesion and the fragmentation regimes. Although, bistability in the adhesion-fragmentation phase diagram of two deformable, charged cells immersed in an ionic aqueous environment has been identified by some in vitro experiments, but until now, has not been quantified theoretically.

Single cell adhesion force measurement for cell viability identification using an AFM cantilever-based micro putter

NASA Astrophysics Data System (ADS)

Shen, Yajing; Nakajima, Masahiro; Kojima, Seiji; Homma, Michio; Kojima, Masaru; Fukuda, Toshio

2011-11-01

Fast and sensitive cell viability identification is a key point for single cell analysis. To address this issue, this paper reports a novel single cell viability identification method based on the measurement of single cell shear adhesion force using an atomic force microscopy (AFM) cantilever-based micro putter. Viable and nonviable yeast cells are prepared and put onto three kinds of substrate surfaces, i.e. tungsten probe, gold and ITO substrate surfaces. A micro putter is fabricated from the AFM cantilever by focused ion beam etching technique. The spring constant of the micro putter is calibrated using the nanomanipulation approach. The shear adhesion force between the single viable or nonviable cell and each substrate is measured using the micro putter based on the nanorobotic manipulation system inside an environmental scanning electron microscope. The adhesion force is calculated based on the deflection of the micro putter beam. The results show that the adhesion force of the viable cell to the substrate is much larger than that of the nonviable cell. This identification method is label free, fast, sensitive and can give quantitative results at the single cell level.

Observations of the Morning Development of the Urban Boundary Layer Over London, UK, Taken During the ACTUAL Project

NASA Astrophysics Data System (ADS)

Halios, Christos H.; Barlow, Janet F.

2018-03-01

The study of the boundary layer can be most difficult when it is in transition and forced by a complex surface, such as an urban area. Here, a novel combination of ground-based remote sensing and in situ instrumentation in central London, UK, is deployed, aiming to capture the full evolution of the urban boundary layer (UBL) from night-time until the fully-developed convective phase. In contrast with the night-time stable boundary layer observed over rural areas, the night-time UBL is weakly convective. Therefore, a new approach for the detection of the morning-transition and rapid-growth phases is introduced, based on the sharp, quasi-linear increase of the mixing height. The urban morning-transition phase varied in duration between 0.5 and 4 h and the growth rate of the mixing layer during the rapid-growth phase had a strong positive relationship with the convective velocity scale, and a weaker, negative relationship with wind speed. Wind shear was found to be higher during the night-time and morning-transition phases than the rapid-growth phase and the shear production of turbulent kinetic energy near the mixing-layer top was around six times larger than surface shear production in summer, and around 1.5 times larger in winter. In summer under low winds, low-level jets dominated the UBL, and shear production was greater than buoyant production during the night-time and the morning-transition phase near the mixing-layer top. Within the rapid-growth phase, buoyant production dominated at the surface, but shear production dominated in the upper half of the UBL. These results imply that regional flows such as low-level jets play an important role alongside surface forcing in determining UBL structure and growth.

The Effect of Two Soft Drinks on Bracket Bond Strength and on Intact and Sealed Enamel: An In Vitro Study

PubMed Central

Pasha, Azam; Sindhu, D; Nayak, Rabindra S; Mamatha, J; Chaitra, K R; Vishwakarma, Swati

2015-01-01

Background and Objectives: This study was conducted to evaluate the effect of two soft drinks, Coca-Cola and Mirinda orange on bracket bond strength, on adhesive remnant on teeth after debonding the bracket, and to observe by means of scanning electron microscope (SEM) the effect of these drinks on intact and sealed enamel. Methods: 120 non-carious maxillary premolar teeth already extracted for Orthodontic purposes were taken and divided into three groups, i.e., Coca-Cola drink, Mirinda orange, and control (artificial saliva) group. Brackets were bonded using conventional methods. Teeth were kept in soft drinks for 15 days, for 15 min, 3 times a day, separated by intervals of 2 h. At other times, they were kept in artificial saliva. The samples, thus obtained were evaluated for shear bond strength using the universal testing machine and subsequently subjected for adhesive remnant index (ARI) scores. SEM study on all the three groups was done for evaluating enamel surface of the intact and sealed enamel. Results: The lowest mean resistance to shearing forces was shown by Mirinda orange group (5.30 ± 2.74 Mpa) followed by Coca-Cola group (6.24 ± 1.59 Mpa) and highest resistance to shearing forces by control group (7.33 ± 1.72 Mpa). The ARI scores revealed a cohesive failure in control samples and an adhesive failure in Mirinda and cola samples. SEM results showed areas of defect due to erosion caused by acidic soft drinks on intact and sealed enamel surface. Conclusion: Mirinda group showed the lowest resistance to shearing forces, followed by Coca-Cola group and with the highest resistance to shearing forces by the control group. There were significant differences between the control group and the study groups. Areas of defects, which were caused by erosion related to acidic soft drinks on the enamel surface around the adhesive, were seen. Areas of defects caused by Coca-Cola were more extensive when compared to Mirinda orange drink. PMID:26668477

New boundary conditions for fluid interaction with hydrophobic surface

NASA Astrophysics Data System (ADS)

Pochylý, František; Fialová, Simona; Havlásek, Michal

2018-06-01

Solution of both laminar and turbulent flow with consideration of hydrophobic surface is based on the original Navier assumption that the shear stress on the hydrophobic surface is directly proportional to the slipping velocity. In the previous work a laminar flow analysis with different boundary conditions was performed. The shear stress value on the tube walls directly depends on the pressure gradient. In the solution of the turbulent flow by the k-ɛ model, the occurrence of the fluctuation components of velocity on the hydrophobic surface is considered. The fluctuation components of the velocity affect the size of the adhesive forces. We assume that the boundary condition for ɛ depending on the velocity gradients will not need to be changed. When the liquid slips over the surface, non-zero fluctuation velocity components occur in the turbulent flow. These determine the non-zero value of the turbulent kinetic energy K. In addition, the fluctuation velocity components also influence the value of the adhesive forces, so it is necessary to include these in the formulation of new boundary conditions for turbulent flow on the hydrophobic surface.

Bone attachment to glass-fibre-reinforced composite implant with porous surface.

PubMed

Mattila, R H; Laurila, P; Rekola, J; Gunn, J; Lassila, L V J; Mäntylä, T; Aho, A J; Vallittu, P K

2009-06-01

A method has recently been developed for producing fibre-reinforced composites (FRC) with porous surfaces, intended for use as load-bearing orthopaedic implants. This study focuses on evaluation of the bone-bonding behaviour of FRC implants. Three types of cylindrical implants, i.e. FRC implants with a porous surface, solid polymethyl methacrylate (PMMA) implants and titanium (Ti) implants, were inserted in a transverse direction into the intercondular trabeculous bone area of distal femurs and proximal tibias of New Zealand White rabbits. Animals were sacrificed at 3, 6 and 12 weeks post operation, and push-out tests (n=5-6 per implant type per time point) were then carried out. At 12 weeks the shear force at the porous FRC-bone interface was significantly higher (283.3+/-55.3N) than the shear force at interfaces of solid PMMA/bone (14.4+/-11.0 N; p<0.001) and Ti/bone (130.6+/-22.2N; p=0.001). Histological observation revealed new bone growth into the porous surface structure of FRC implants. Solid PMMA and Ti implants were encapsulated mostly with fibrous connective tissue. Finite element analysis (FEA) revealed that porous FRC implants had mechanical properties which could be tailored to smooth the shear stress distribution at the bone-implant interface and reduce the stress-shielding effect.

Torsional bridge setup for the characterization of integrated circuits and microsensors under mechanical shear stress.

PubMed

Herrmann, M; Gieschke, P; Ruther, P; Paul, O

2011-12-01

We present a torsional bridge setup for the electro-mechanical characterization of devices integrated in the surface of silicon beams under mechanical in-plane shear stress. It is based on the application of a torsional moment to the longitudinal axis of the silicon beams, which results in a homogeneous in-plane shear stress in the beam surface. The safely applicable shear stresses span the range of ±50 MPa. Thanks to a specially designed clamping mechanism, the unintended normal stress typically stays below 2.5% of the applied shear stress. An analytical model is presented to compute the induced shear stress. Numerical computations verify the analytical results and show that the homogeneity of the shear stress is very high on the beam surface in the region of interest. Measurements with piezoresistive microsensors fabricated using a complementary metal-oxide-semiconductor process show an excellent agreement with both the computational results and comparative measurements performed on a four-point bending bridge. The electrical connection to the silicon beam is performed with standard bond wires. This ensures that minimal forces are applied to the beam by the electrical interconnection to the external instrumentation and that devices with arbitrary bond pad layout can be inserted into the setup.

Quasi-Equilibrium States in the Tropics Simulated by a Cloud-Resolving Model. Part 1; Specific Features and Budget Analysis

NASA Technical Reports Server (NTRS)

Shie, C.-L.; Tao, W.-K.; Simpson, J.; Sui, C.-H.; Starr, David OC. (Technical Monitor)

2001-01-01

A series of long-term integrations using the two-dimensional Goddard Cumulus Ensemble (GCE) model were performed by altering imposed environmental components to produce various quasi-equilibrium thermodynamic states. Model results show that the genesis of a warm/wet quasi-equilibrium state is mainly due to either strong vertical wind shear (from nudging) or large surface fluxes (from strong surface winds), while a cold/dry quasi-equilibrium state is attributed to a remarkably weakened mixed-wind shear (from vertical mixing due to deep convection) along with weak surface winds. In general, latent heat flux and net large-scale temperature forcing, the two dominant physical processes, dominate in the beginning stage of the simulated convective systems, then considerably weaken in the final stage, which leads to quasi-equilibrium states. A higher thermodynamic regime is found to produce a larger rainfall amount, as convective clouds are the leading source of rainfall over stratiform clouds even though the former occupy much less area. Moreover, convective clouds are more likely to occur in the presence of strong surface winds (latent heat flux), while stratiform clouds (especially the well-organized type) are favored in conditions with strong wind shear (large-scale forcing). The convective systems, which consist of distinct cloud types due to the variation in horizontal winds, are also found to propagate differently. Accordingly, convective systems with mixed-wind shear generally propagate in the direction of shear, while the system with strong (multidirectional) wind shear propagates in a more complex way. Based on the results from the temperature (Q1) and moisture (Q2) budgets, cloud-scale eddies are found to act as a hydrodynamic 'vehicle' that cascades the heat and moisture vertically. Several other specific features such as atmospheric stability, CAPE, and mass fluxes are also investigated and found to be significantly different between diverse quasi-equilibrium states. Detailed comparisons between the various states are presented.

Oscillatory shear rheology measurements and Newtonian modeling of insoluble monolayers

NASA Astrophysics Data System (ADS)

Rasheed, Fayaz; Raghunandan, Aditya; Hirsa, Amir H.; Lopez, Juan M.

2017-04-01

Circular systems are advantageous for interfacial studies since they do not suffer from end effects, but their hydrodynamics is more complicated because their flows are not unidirectional. Here, we analyze the shear rheology of a harmonically driven knife-edge viscometer through experiments and computations based on the Navier-Stokes equations with a Newtonian interface. The measured distribution of phase lag in the surface velocity relative to the knife-edge speed is found to have a good signal-to-noise ratio and provides robust comparisons to the computations. For monomolecular films of stearic acid, the surface shear viscosity deduced from the model was found to be the same whether the film is driven steady or oscillatory, for an order of magnitude range in driving frequencies and amplitudes. Results show that increasing either the amplitude or forcing frequency steepens the phase lag next to the knife edge. In all cases, the phase lag is linearly proportional to the radial distance from the knife edge and scales with surface shear viscosity to the power -1 /2 .

Laser reflection method for determination of shear stress in low density transitional flows

NASA Astrophysics Data System (ADS)

Sathian, Sarith P.; Kurian, Job

2006-03-01

The details of laser reflection method (LRM) for the determination of shear stress in low density transitional flows are presented. The method is employed to determine the shear stress due to impingement of a low density supersonic free jet issuing out from a convergent divergent nozzle on a flat plate. The plate is smeared with a thin oil film and kept parallel to the nozzle axis. For a thin oil film moving under the action of aerodynamic boundary layer, the shear stress at the air-oil interface is equal to the shear stress between the surface and air. A direct and dynamic measurement of the oil film slope generated by the shear force is done using a position sensing detector (PSD). The thinning rate of the oil film is directly measured which is the major advantage of the LRM. From the oil film slope history, calculation of the shear stress is done using a three-point formula. The range of Knudsen numbers investigated is from 0.028 to 0.516. Pressure ratio across the nozzle varied from 3,500 to 8,500 giving highly under expanded free jets. The measured values of shear, in the overlapping region of experimental parameters, show fair agreement with those obtained by force balance method and laser interferometric method.

Crust-mantle mechanical coupling in Eastern Mediterranean and Eastern Turkey

PubMed Central

Sinan Özeren, M.

2012-01-01

Present-day crust-mantle coupling in the Eastern Mediterranean and eastern Turkey is studied using the Global Positioning System (GPS) and seismic anisotropy data. The general trend of the shear wave fast-splitting directions in NE Turkey and Lesser Caucaus align well with the geodetic velocities in an absolute plate motion frame of reference pointing to an effective coupling in this part of the region of weak surface deformation. Farther south, underneath the Bitlis Suture, however, there are significant Pn delays with E-W anisotropy axes indicating significant lateral escape. Meanwhile, the GPS reveals very little surface deformation. This mismatch possibly suggests a decoupling along the suture. In the Aegean, the shear wave anisotropy and the Pn anisotropy directions agree with the extensional component of the right-lateral shear strains except under the Crete Basin and other parts of the southern Aegean Sea. This extensional direction matches perfectly also with the southward pulling force vectors across the Hellenic trench; however, the maximum right-lateral shear directions obtained from the GPS data in the Aegean do not match either of these anisotropies. Seismic anisotropy from Rayleigh waves sampled at 15 s, corresponding to the lower crust, match the maximum right-lateral maximum shear directions from the GPS indicating decoupling between the crust and the mantle. This decoupling most likely results from the lateral variations of the gravitational potential energies and the slab-pull forces. PMID:22592788

Crust-mantle mechanical coupling in Eastern Mediterranean and eastern Turkey.

PubMed

Özeren, M Sinan

2012-05-29

Present-day crust-mantle coupling in the Eastern Mediterranean and eastern Turkey is studied using the Global Positioning System (GPS) and seismic anisotropy data. The general trend of the shear wave fast-splitting directions in NE Turkey and Lesser Caucaus align well with the geodetic velocities in an absolute plate motion frame of reference pointing to an effective coupling in this part of the region of weak surface deformation. Farther south, underneath the Bitlis Suture, however, there are significant Pn delays with E-W anisotropy axes indicating significant lateral escape. Meanwhile, the GPS reveals very little surface deformation. This mismatch possibly suggests a decoupling along the suture. In the Aegean, the shear wave anisotropy and the Pn anisotropy directions agree with the extensional component of the right-lateral shear strains except under the Crete Basin and other parts of the southern Aegean Sea. This extensional direction matches perfectly also with the southward pulling force vectors across the Hellenic trench; however, the maximum right-lateral shear directions obtained from the GPS data in the Aegean do not match either of these anisotropies. Seismic anisotropy from Rayleigh waves sampled at 15 s, corresponding to the lower crust, match the maximum right-lateral maximum shear directions from the GPS indicating decoupling between the crust and the mantle. This decoupling most likely results from the lateral variations of the gravitational potential energies and the slab-pull forces.

Optimizing Micromixer Surfaces To Deter Biofouling.

PubMed

Waters, James T; Liu, Ya; Li, Like; Balazs, Anna C

2018-03-07

Using computational modeling, we show that the dynamic interplay between a flowing fluid and the appropriately designed surface relief pattern can inhibit the fouling of the substrate. We specifically focus on surfaces that are decorated with three-dimensional (3D) chevron or sawtooth "micromixer" patterns and model the fouling agents (e.g., cells) as spherical microcapsules. The interaction between the imposed shear flow and the chevrons on the surface generates 3D vortices in the system. We pinpoint a range of shear rates where the forces from these vortices can rupture the bonds between the two mobile microcapsules near the surface. Notably, the patterned surface offers fewer points of attachment than a flat substrate, and the shear flows readily transport the separated capsules away from the layer. We contrast the performance of surfaces that encompass rectangular posts, chevrons, and asymmetric sawtooth patterns and thereby identify the geometric factors that cause the sawtooth structure to be most effective at disrupting the bonding between the capsules. By breaking up nascent clusters of contaminant cells, these 3D relief patterns can play a vital role in disrupting the biofouling of surfaces immersed in flowing fluids.

Prediction of static friction coefficient in rough contacts based on the junction growth theory

NASA Astrophysics Data System (ADS)

Spinu, S.; Cerlinca, D.

2017-08-01

The classic approach to the slip-stick contact is based on the framework advanced by Mindlin, in which localized slip occurs on the contact area when the local shear traction exceeds the product between the local pressure and the static friction coefficient. This assumption may be too conservative in the case of high tractions arising at the asperities tips in the contact of rough surfaces, because the shear traction may be allowed to exceed the shear strength of the softer material. Consequently, the classic frictional contact model is modified in this paper so that gross sliding occurs when the junctions formed between all contacting asperities are independently sheared. In this framework, when the contact tractions, normal and shear, exceed the hardness of the softer material on the entire contact area, the material of the asperities yields and the junction growth process ends in all contact regions, leading to gross sliding inception. This friction mechanism is implemented in a previously proposed numerical model for the Cattaneo-Mindlin slip-stick contact problem, which is modified to accommodate the junction growth theory. The frictionless normal contact problem is solved first, then the tangential force is gradually increased, until gross sliding inception. The contact problems in the normal and in the tangential direction are successively solved, until one is stabilized in relation to the other. The maximum tangential force leading to a non-vanishing stick area is the static friction force that can be sustained by the rough contact. The static friction coefficient is eventually derived as the ratio between the latter friction force and the normal force.

Simulation of a sensor array for multiparameter measurements at the prosthetic limb interface

NASA Astrophysics Data System (ADS)

Rowe, Gabriel I.; Mamishev, Alexander V.

2004-07-01

Sensitive skin is a highly desired device for biomechanical devices, wearable computing, human-computer interfaces, exoskeletons, and, most pertinent to this paper, for lower limb prosthetics. The measurement of shear stress is very important because shear effects are key factors in developing surface abrasions and pressure sores in paraplegics and users of prosthetic/orthotic devices. A single element of a sensitive skin is simulated and characterized in this paper. Conventional tactile sensors are designed for measurement of the normal stress only, which is inadequate for comprehensive assessment of surface contact conditions. The sensitive skin discussed here is a flexible array capable of sensing shear and normal forces, as well as humidity and temperature on each element.

Dielectrophoretic levitation in the presence of shear flow: implications for colloidal fouling of filtration membranes.

PubMed

Molla, Shahnawaz; Bhattacharjee, Subir

2007-10-09

The ability of dielectrophoretic (DEP) forces created using a microelectrode array to levitate particles in a colloidal suspension is studied experimentally and theoretically. The experimental system employs microfabricated electrode arrays on a glass substrate to apply repulsive DEP forces on polystyrene latex particles suspended in an aqueous medium. A numerical model based on the convection-diffusion-migration equation is presented to calculate the concentration distribution of colloidal particles in shear flow under the influence of a repulsive DEP force field. The results obtained from the numerical simulations are compared against trajectory analysis results and experimental data. The results indicate that by incorporating ac electric field-induced DEP forces in a shear flow, particle accumulation and deposition on the flow channel surfaces can be significantly reduced or even completely averted. The mathematical model is then used to indicate how the deposition behavior is modified in the presence of a permeable substrate, representative of tangential flow membrane filtration operations. The results indicate that the repulsive dielectrophoretic (DEP) forces imparted to the particles suspended in the feed can be employed to mitigate membrane fouling in a cross-flow filtration process.

Influence of surface treatment on shear bond strength of orthodontic brackets.

PubMed

Brunharo, Ione Helena Vieira Portella; Fernandes, Daniel Jogaib; de Miranda, Mauro Sayão; Artese, Flavia

2013-01-01

The shear bond strength of orthodontic brackets bonded to micro-hybrid and micro-particulate resins under different surface treatment methods was assessed. Two hundred and eighty test samples were divided into 28 groups (n = 10), where 140 specimens were filled with Durafill micro-particulate resin and 140 with Charisma composite. In 140 samples, a coupling agent (silane) was applied. The surface treatment methods were: Phosphoric and hydrofluoric acid etching, sodium bicarbonate and aluminum oxide blasting, stone and burs. A Universal Instron Machine was used to apply an occlusal shear force directly to the resin composite bracket surface at a speed of 0.5 mm/min. The means were compared using analysis of variance and multivariate regression to assess the interaction between composites and surface treatment methods. Means and standard deviations for the groups were: Sodium bicarbonate jet 11.27 ± 2.78; burs 9.26 ± 3.01; stone 7.95 ± 3.67; aluminum oxide blasting 7.04 ± 3.21; phosphoric acid 5.82 ± 1.90; hydrofluoric acid 4.54 ± 2.87, and without treatment 2.75 ± 1.49. An increase of 1.94 MPa in shear bond strength was seen in Charisma groups. Silane agent application reduced the Charisma shear bond strength by 0.68 Mpa, but increased Durafill means for bicarbonate blasting (0.83), burs (0.98) and stone drilling (0.46). The sodium bicarbonate blasting, burs and stone drilling methods produced adequate shear bond strength and may be suitable for clinical use. The Charisma micro hybrid resin composite showed higher shear bond means than Durafill micro particle composite.

CFD Analysis of Flexible Thermal Protection System Shear Configuration Testing in the LCAT Facility

NASA Technical Reports Server (NTRS)

Ferlemann, Paul G.

2014-01-01

This paper documents results of computational analysis performed after flexible thermal protection system shear configuration testing in the LCAT facility. The primary objectives were to predict the shear force on the sample and the sensitivity of all surface properties to the shape of the sample. Bumps of 0.05, 0.10,and 0.15 inches were created to approximate the shape of some fabric samples during testing. A large amount of information was extracted from the CFD solutions for comparison between runs and also current or future flight simulations.

Lift and drag forces on an inclined plow moving over a granular surface.

PubMed

Percier, Baptiste; Manneville, Sebastien; McElwaine, Jim N; Morris, Stephen W; Taberlet, Nicolas

2011-11-01

We studied the drag and lift forces acting on an inclined plate while it is dragged on the surface of a granular media, both in experiment and in numerical simulation. In particular, we investigated the influence of the horizontal velocity of the plate and its angle of attack. We show that a steady wedge of grains is moved in front of the plow and that the lift and drag forces are proportional to the weight of this wedge. These constants of proportionality vary with the angle of attack but not (or only weakly) on the velocity. We found a universal effective friction law that accounts for the dependence on all the above-mentioned parameters. The stress and velocity fields are calculated from the numerical simulations and show the existence of a shear band under the wedge and that the pressure is nonhydrostatic. The strongest gradients in stress and shear occur at the base of the plow where the dissipation rate is therefore highest.

Surface Stresses and a Force Balance at a Contact Line.

PubMed

Liang, Heyi; Cao, Zhen; Wang, Zilu; Dobrynin, Andrey V

2018-06-26

Results of the coarse-grained molecular dynamics simulations are used to show that the force balance analysis at the triple-phase contact line formed at an elastic substrate has to include a quartet of forces: three surface tensions (surface free energies) and an elastic force per unit length. In the case of the contact line formed by a droplet on an elastic substrate an elastic force is due to substrate deformation generated by formation of the wetting ridge. The magnitude of this force f el is proportional to the product of the ridge height h and substrate shear modulus G. Similar elastic line force should be included in the force analysis at the triple-phase contact line of a solid particle in contact with an elastic substrate. For this contact problem elastic force obtained from contact angles and surface tensions is a sum of the elastic forces acting from the side of a solid particle and an elastic substrate. By considering only three line forces acting at the triple-phase contact line, one implicitly accounts the bulk stress contribution as a part of the resultant surface stresses. This "contamination" of the surface properties by a bulk contribution could lead to unphysically large values of the surface stresses in soft materials.

Shear bond strength of a new self-adhering flowable composite resin for lithium disilicate-reinforced CAD/CAM ceramic material

PubMed Central

Sancakli, Hande Sar; Sancakli, Erkan; Eren, Meltem Mert; Ozel, Sevda; Yucel, Taner; Yildiz, Esra

2014-01-01

PURPOSE The purpose of this study was to evaluate and compare the effects of different surface pretreatment techniques on the surface roughness and shear bond strength of a new self-adhering flowable composite resin for use with lithium disilicate-reinforced CAD/CAM ceramic material. MATERIALS AND METHODS A total of one hundred thirty lithium disilicate CAD/CAM ceramic plates with dimensions of 6 mm × 4 mm and 3 mm thick were prepared. Specimens were then assigned into five groups (n=26) as follows: untreated control, coating with 30 µm silica oxide particles (Cojet™ Sand), 9.6% hydrofluoric acid etching, Er:YAG laser irradiation, and grinding with a high-speed fine diamond bur. A self-adhering flowable composite resin (Vertise Flow) was applied onto the pre-treated ceramic plates using the Ultradent shear bond Teflon mold system. Surface roughness was measured by atomic force microscopy. Shear bond strength test were performed using a universal testing machine at a crosshead speed of 1 mm/min. Surface roughness data were analyzed by one-way ANOVA and the Tukey HSD tests. Shear bond strength test values were analyzed by Kruskal-Wallis and Mann-Whitney U tests at α=.05. RESULTS Hydrofluoric acid etching and grinding with high-speed fine diamond bur produced significantly higher surface roughness than the other pretreatment groups (P<.05). Hydrofluoric acid etching and silica coating yielded the highest shear bond strength values (P<.001). CONCLUSION Self-adhering flowable composite resin used as repair composite resin exhibited very low bond strength irrespective of the surface pretreatments used. PMID:25551002

Wet-spinning fabrication of shear-patterned alginate hydrogel microfibers and the guidance of cell alignment

PubMed Central

Yang, You; Sun, Jing; Liu, Xiaolu; Guo, Zhenzhen; He, Yunhu; Wei, Dan; Zhong, Meiling; Guo, Likun; Zhang, Xingdong

2017-01-01

Abstract Native tissue is naturally comprised of highly-ordered cell-matrix assemblies in a multi-hierarchical way, and the nano/submicron alignment of fibrous matrix is found to be significant in supporting cellular functionalization. In this study, a self-designed wet-spinning device appended with a rotary receiving pool was used to continuously produce shear-patterned hydrogel microfibers with aligned submicron topography. The process that the flow-induced shear force reshapes the surface of hydrogel fiber into aligned submicron topography was systematically analysed. Afterwards, the effect of fiber topography on cellular longitudinal spread and elongation was investigated by culturing rat neuron-like PC12 cells and human osteosarcoma MG63 cells with the spun hydrogel microfibers, respectively. The results suggested that the stronger shear flow force would lead to more distinct aligned submicron topography on fiber surface, which could induce cell orientation along with fiber axis and therefore form the cell-matrix dual-alignment. Finally, a multi-hierarchical tissue-like structure constructed by dual-oriented cell-matrix assemblies was fabricated based on this wet-spinning method. This work is believed to be a potentially novel biofabrication scheme for bottom-up constructing of engineered linear tissue, such as nerve bundle, cortical bone, muscle and hepatic cord. PMID:29026644

The components of shear stress affecting insect cells used with the baculovirus expression vector system.

PubMed

Weidner, Tobias; Druzinec, Damir; Mühlmann, Martina; Buchholz, Rainer; Czermak, Peter

2017-09-26

Insect-based expression platforms such as the baculovirus expression vector system (BEVS) are widely used for the laboratory- and industrial-scale production of recombinant proteins. Thereby, major drawbacks to gain high-quality proteins are the lytic infection cycle and the shear sensitivity of infected insect cells due to turbulence and aeration. Smaller bubbles were formerly assumed to be more harmful than larger ones, but we found that cell damage is also dependent on the concentration of protective agents such as Pluronic®. At the appropriate concentration, Pluronic forms a layer around air bubbles and hinders the attachment of cells, thus limiting the damage. In this context, we used microaeration to vary bubble sizes and confirmed that size is not the most important factor, but the total gas surface area in the reactor is. If the surface area exceeds a certain threshold, the concentration of Pluronic is no longer sufficient for cell protection. To investigate the significance of shear forces, a second study was carried out in which infected insect cells were cultivated in a hollow fiber module to protect them from shear forces. Both model studies revealed important aspects of the design and scale-up of BEVS processes for the production of recombinant proteins.

Lubricant dynamics under sliding condition in disk drives

NASA Astrophysics Data System (ADS)

Wu, Lin

2006-07-01

In this paper, we develop a two-dimensional flow model for the lubricant flow dynamics under a sliding head in disk drives. Our two-dimensional model includes important physics such as viscous force, external air shearing stress, air bearing pressure, centrifugal force, disjoining pressure, and surface tension. Our analysis shows that the lubricant flow dynamics under the sliding condition is a fully two-dimensional phenomenon and the circumferential lubricant flow is strongly coupled to the radial flow. It is necessary to have a two-dimensional flow model that couples the circumferential and radial flows together and includes all important physics to achieve realistic predictions. Our results show that the external air shearing stress has a dominant effect on the lubricant flow dynamics. Both velocity slippage at wall and Poiseuille flow effects have to be considered in the evaluation of the air shearing stress under the head. The nonuniform air bearing pressure has a non-negligible effect on the lubricant film dynamics mostly through the Poiseuille flow effect on the air shearing stress but not from its direct pushing or sucking effect on the lubricant surface. Prediction of the formation of lubricant depletion tracks under a sliding head using the two-dimensional model agrees reasonably well with the existing experimental measurements.

Correlation of fingertip shear force direction with somatosensory cortical activity in monkey

PubMed Central

Fortier-Poisson, Pascal; Langlais, Jean-Sébastien

2015-01-01

To examine the activity of somatosensory cortex (S1) neurons to self-generated shear forces on the index and thumb, two monkeys were trained to grasp a stationary metal tab with a key grip and exert forces without the fingers slipping in one of four orthogonal directions for 1 s. A majority (∼85%) of slowly adapting and rapidly adapting (RA) S1 neurons had activity modulated with shear force direction. The cells were recorded mainly in areas 1 and 2 of the S1, although some area 3b neurons also responded to shear direction or magnitude. The preferred shear vectors were distributed in every direction, with tuning arcs varying from 50° to 170°. Some RA neurons sensitive to dynamic shear force direction also responded to static shear force but within a narrower range, suggesting that the direction of the shear force may influence the adaptation rate. Other neurons were modulated with shear forces in diametrically opposite directions. The directional sensitivity of S1 cortical neurons is consistent with recordings from cutaneous afferents showing that shear direction, even without slip, is a powerful stimulus to S1 neurons. PMID:26467520

The Effect of Humidity and Particle Characteristics on Friction and Stick-slip Instability in Granular Fault Gouge

NASA Astrophysics Data System (ADS)

Anthony, J. L.; Marone, C. J.

2003-12-01

Previous studies have shown that particle characteristics such as shape, dimension, and roughness affect friction in granular shear zones. Other work shows that humidity plays a key role in frictional healing and rate/state dependence within granular gouge. In order to improve our understanding of grain-scale deformation mechanisms within fault gouge, we performed laboratory experiments using a double-direct-shear testing apparatus. This assembly includes three rigid forcing blocks with two gouge layers sandwiched between rough or smooth surfaces. Roughened surfaces were triangular grooves 0.8 mm deep and 1 mm wavelength. These promote distributed shear throughout the layer undergoing cataclastic deformation. Smooth surfaces were mirror-finished hardened steel and were used to promote and isolate grain boundary sliding. The center block is forced at controlled displacement rate between the two side blocks to create frictional shear. We studied gouge layers 3-7 mm thick, consisting of either quartz rods sheared in 1-D and 2-D configurations and smooth glass beads mixed with varying amounts of rough sand particles. We report on particle diameters that range from 0.050-0.210 mm, and quartz rods 1 mm in diameter and 100 mm long. The experiments are run at room temperature, controlled relative humidity ranging from 5 to 100%, and shear displacement rates from 0.1 to 300 microns per second. Experiments are carried out under a normal stress of 5 MPa, a non-fracture loading regime where sliding friction for smooth spherical particles is measurably lower than for rough angular particles. We compare results from shear between smooth boundaries, where we hypothesize that grain boundary sliding is the mechanism influencing granular friction, to rough sample experiments where shear undergoes a transition from distributed, pervasive shear to progressively localized as a function of increasing strain. For shear within rough surfaces, stick-slip instability occurs in gouge that consists of less than 30% angular grains and begins once the coefficient of friction (shear stress divided by normal stress) reaches a value of 0.35-0.40. Peak friction during stick-slip cycles is 0.40-0.45. Each stick-slip event involves a small amount of quasi-static displacement prior to failure, which we refer to as pre-seismic slip. For unstable sliding regimes, we measure the amount of pre-seismic slip and the magnitude of dynamic stress drop. These parameters vary systematically with sliding velocity, particle characteristics, and bounding roughness. For shear within smooth surfaces, friction is very low (0.15-0.16 for spherical particles) and sliding is stable, without stick-slip instability. As more angular grains are mixed with spherical beads the coefficient of friction increases. This holds true for both the rough and smooth sample experiments. We expand on previous work done by Frye and Marone 2002 (JGR) to study the effect of humidity on 1-D, 2-D, and 3-D gouge layer configurations. Our data show that humidity has a significant effect on frictional strength and stability and that this effect is observed for both smooth surfaces, where grain boundary sliding is the dominant deformation mechanisms, and for shear within rough surfaces where gouge deformation occurs by rolling, dilation, compaction, and grain boundary sliding.

Influence of surface treatments on the shear bond strength of orthodontic brackets to porcelain

NASA Astrophysics Data System (ADS)

Wang, Cong; Zeng, Jishan; Wang, Shaoan; Yang, Zheng; Huang, Qian; Chen, Pixiu; Zhou, Shujuan; Liu, Xiaoqing

2008-11-01

The purpose of this study was to investigate the effect of various surface treatments after different storage time and thermocycling on the shear bond strength of orthodontic brackets to the feldspathic porcelain surfaces. 128 disc-shaped porcelain specimens were randomly assigned to the following surface treatments: 9.6% HFA, 9.6% HFA combined with silane, 50 μ aluminum trioxide sandblasting followed by silane and application of silane after 37% phosphoric acid. Metal or ceramic brackets were bonded onto each treated porcelain facet with light cured resin. The samples were stored in 37 °C water 1 day or 7 days, thermocycled 500 times from 5 to 55 °C. The shear bond strengths were measured (1 mm/min), and statistically analyzed. The bond failure sites were classified according to ARI system. The surface of the glazed, sandblasted, hydrofluoric and phosphoric acid etched porcelain were examined with SEM. All groups achieved reasonable bond strengths to withstand the application of orthodontic forces. Water storage for 7 days caused lower shear bond strength than that of 1 day. But there is no statistically significant difference between the two groups. The mean shear bond strength provided by ceramic bracket with mechanical retention had no statistical difference with that of metal bracket. Therefore, the optimal treatment for orthodontic brackets bonding to feldspathic porcelain was to apply phosphoric acid combined with silane.

Particle-wall tribology of slippery hydrogel particle suspensions.

PubMed

Shewan, Heather M; Stokes, Jason R; Cloitre, Michel

2017-03-08

Slip is an important phenomenon that occurs during the flow of yield stress fluids like soft materials and pastes. Densely packed suspensions of hydrogel microparticles are used to show that slip is governed by the tribological interactions occurring between the samples and shearing surfaces. Both attractive/repulsive interactions between the dispersed particles and surface, as well as the viscoelasticity of the suspension, are found to play key roles in slip occurring within rheometric flows. We specifically discover that for two completely different sets of microgels, the sliding stress at which slip occurs scales with both the modulus of the particles and the bulk suspension modulus. This suggests that hysteresis losses within the viscoelastic particles contribute to friction forces and thus slip at the particle-surface tribo-contact. It is also found that slip during large amplitude oscillatory shear and steady shear flows share the same generic features.

Constitutive law for the densification of fused silica with applications in polishing and microgrinding

NASA Astrophysics Data System (ADS)

Lambropoulos, John C.; Fang, Tong; Xu, Su; Gracewski, Sheryl M.

1995-09-01

We discuss a constitutive model describing the permanent densification of fused silica under large applied pressures and shear stresses. The constitutive law is assumed to be rate- independent, and uses a yield function coupling hydrostatic pressure and shear stress, a flow rule describing the evolution of permanent strains after initial densification, and a hardening rule describing the dependence of the incremental densification on the levels of applied stresses. The constitutive law accounts for multiaxial states of stress, since during polishing and grinding operations complex stress states occur in a thin surface layer due to the action of abrasive particles. Due to frictional and other abrasive forces, large shear stresses are present near the surface during manufacturing. We apply the constitutive law in estimating the extent of the densified layer during the mechanical interaction of an abrasive grain and a flat surface.

Effect of Reduced Phosphoric Acid Pre-etching Times 
on Enamel Surface Characteristics and Shear Fatigue Strength Using Universal Adhesives.

PubMed

Tsujimoto, Akimasa; Fischer, Nicholas; Barkmeier, Wayne; Baruth, Andrew; Takamizawa, Toshiki; Latta, Mark; Miyazaki, Masashi

2017-01-01

To examine the effect of reduced phosphoric acid pre-etching times on enamel fatigue bond strength of universal adhesives and surface characteristics by using atomic force microscopy (AFM). Three universal adhesives were used in this study (Clearfil Universal Bond [C], G-Premio Bond [GP], Scotchbond Universal Adhesive [SU]). Four pre-etching groups were employed: enamel pre-etched with phosphoric acid and immediately rinsed with an air-water spray, and enamel pre-etched with phosphoric acid for 5, 10, or 15 s. Ground enamel was used as the control group. For the initial bond strength test, 15 specimens per etching group for each adhesive were used. For the shear fatigue test, 20 specimens per etching group for each adhesive were loaded using a sine wave at a frequency of 20 Hz for 50,000 cycles or until failure occurred. Initial shear bond strengths and fatigue shear strengths of composite adhesively bonded to ground and pre-etched enamel were determined. AFM observations of ground and pre-etched enamel were also conducted, and surface roughness as well as surface area were evaluated. The initial shear bond strengths and fatigue shear strengths of the universal adhesives in the pre-etched groups were significantly higher than those of the control group, and were not influenced by the pre-etching time. Significantly higher surface roughness and surface area of enamel surfaces in pre-etched groups were observed compared with those in the control group. While the surface area was not significantly influenced by etching time, surface roughness of the enamel surfaces in the pre-etched groups significantly increased with pre-etching time. The results of this in vitro study suggest that reduced phosphoric acid pre-etching times do not impair the fatigue bond strength of universal adhesives. Although fatigue bond strength and surface area were not influenced by phosphoric-acid etching times, surface roughness increased with increasing etching time.

Capture and On-chip analysis of Melanoma Cells Using Tunable Surface Shear forces

NASA Astrophysics Data System (ADS)

Tsao, Simon Chang-Hao; Vaidyanathan, Ramanathan; Dey, Shuvashis; Carrascosa, Laura G.; Christophi, Christopher; Cebon, Jonathan; Shiddiky, Muhammad J. A.; Behren, Andreas; Trau, Matt

2016-01-01

With new systemic therapies becoming available for metastatic melanoma such as BRAF and PD-1 inhibitors, there is an increasing demand for methods to assist with treatment selection and response monitoring. Quantification and characterisation of circulating melanoma cells (CMCs) has been regarded as an excellent non-invasive candidate but a sensitive and efficient tool to do these is lacking. Herein we demonstrate a microfluidic approach for melanoma cell capture and subsequent on-chip evaluation of BRAF mutation status. Our approach utilizes a recently discovered alternating current electrohydrodynamic (AC-EHD)-induced surface shear forces, referred to as nanoshearing. A key feature of nanoshearing is the ability to agitate fluid to encourage contact with surface-bound antibody for the cell capture whilst removing nonspecific cells from the surface. By adjusting the AC-EHD force to match the binding affinity of antibodies against the melanoma-associated chondroitin sulphate proteoglycan (MCSP), a commonly expressed melanoma antigen, this platform achieved an average recovery of 84.7% from biological samples. Subsequent staining with anti-BRAFV600E specific antibody enabled on-chip evaluation of BRAFV600E mutation status in melanoma cells. We believe that the ability of nanoshearing-based capture to enumerate melanoma cells and subsequent on-chip characterisation has the potential as a rapid screening tool while making treatment decisions.

The mean and turbulent flow structure of a weak hydraulic jump

NASA Astrophysics Data System (ADS)

Misra, S. K.; Kirby, J. T.; Brocchini, M.; Veron, F.; Thomas, M.; Kambhamettu, C.

2008-03-01

The turbulent air-water interface and flow structure of a weak, turbulent hydraulic jump are analyzed in detail using particle image velocimetry measurements. The study is motivated by the need to understand the detailed dynamics of turbulence generated in steady spilling breakers and the relative importance of the reverse-flow and breaker shear layer regions with attention to their topology, mean flow, and turbulence structure. The intermittency factor derived from turbulent fluctuations of the air-water interface in the breaker region is found to fit theoretical distributions of turbulent interfaces well. A conditional averaging technique is used to calculate ensemble-averaged properties of the flow. The computed mean velocity field accurately satisfies mass conservation. A thin, curved shear layer oriented parallel to the surface is responsible for most of the turbulence production with the turbulence intensity decaying rapidly away from the toe of the breaker (location of largest surface curvature) with both increasing depth and downstream distance. The reverse-flow region, localized about the ensemble-averaged free surface, is characterized by a weak downslope mean flow and entrainment of water from below. The Reynolds shear stress is negative in the breaker shear layer, which shows that momentum diffuses upward into the shear layer from the flow underneath, and it is positive just below the mean surface indicating a downward flux of momentum from the reverse-flow region into the shear layer. The turbulence structure of the breaker shear layer resembles that of a mixing layer originating from the toe of the breaker, and the streamwise variations of the length scale and growth rate are found to be in good agreement with observed values in typical mixing layers. All evidence suggests that breaking is driven by a surface-parallel adverse pressure gradient and a streamwise flow deceleration at the toe of the breaker. Both effects force the shear layer to thicken rapidly, thereby inducing a sharp free surface curvature change at the toe.

Evaluating the point of separation, during carcass fabrication, between the beef wholesale rib and the beef wholesale chuck.

PubMed

Reuter, B J; Wulf, D M; Shanks, B C; Maddock, R J

2002-01-01

This study determined whether there is a logical point of value change, related to either tenderness or consumer acceptance, at which to separate the beef carcass within the rib/chuck region. Rib/chuck rolls (RCR); (n = 30) consisting of the ribeye roll and chuck eye roll subprimals (2nd through 12th rib locations) were cut into 22 steaks each (two steaks per rib location), and Warner-Bratzler shear force and consumer purchase preference were evaluated for steaks at each rib location. Steaks from different locations of the RCR were composed of differing proportions of several muscles: longissimus muscle (LM), spinalis dorsi and multifidus dorsi (SM), and complexus (CO). The LM (4th to 12th rib) contained three tenderness regions: 7th through 12th rib, 5th and 6th ribs, and 4th rib regions (lowest, intermediate, and highest shear force values, respectively; P < 0.01). Shear force differed (P < 0.05) among rib locations for the SM (2nd to 9th rib), but no logical pattern was evident. The CO (2nd to 7th rib) was more tender toward the anterior end (P < 0.05). The region of the RCR represented by the 4th through 6th rib locations had steaks with higher weighted-average shear force (average shear force of each steak, weighted for surface area of each muscle) values than the remainder of the RCR (P < 0.05). Animal-to-animal variation in shear force was 36% greater than rib-to-rib variation in shear force; thus, statistically significant differences in tenderness among rib locations may be undetectable by consumers. Steaks (n = 330) were offered for sale at a retail supermarket and case time was monitored on each steak to determine consumer purchase preference. Steaks from the 2nd through 4th rib locations required more time to sell (P < 0.01) than steaks from the 5th through 12th rib locations. Two alternative locations for the rib/chuck separation point could be between the 6th and 7th ribs, yielding a ribeye subprimal useful in marketing a "premium quality" product, or between the 4th and 5th ribs, which would yield four more 2.5-cm ribeye steaks per carcass.

Wind Shear Effects on the Structure and Dynamics of the Daytime Atmospheric Boundary Layer

NASA Astrophysics Data System (ADS)

Haghshenas, Armin; Mellado, Juan Pedro

2017-04-01

The daytime atmospheric boundary layer (ABL), in which the positive buoyancy flux at the surface creates convective instability and generates turbulence, has been a subject of extensive research during the last century. However, fewer studies have considered wind shear in detail and most of them are single-case studies. So most of the available theories and parameterizations have not been sufficiently tested over a wide range of atmospheric conditions. Moreover, since previous numerical studies were mostly carried out by large eddy simulation, a complete understanding of the physics of the problem is still missing due to the lack of information about the small-scale dynamics. Specifically, despite the consensus in the community that wind shear enhances the entrainment process, the amount of enhancement is still matter of contention. In order to investigate the effects of wind shear on the structure and dynamics of the ABL in detail, direct numerical simulations are used in this study. Shear is prescribed by a height-constant velocity in the troposphere and the simulation runs until a fully turbulent, quasi-equilibrium regime is observed. Despite the simplification of neglecting the Coriolis force, our configuration reproduces the main features observed in the previous studies, which had taken the Coriolis force into account. As a novelty compared to previous single-case studies, we introduce a dimensionless parameter that allows us to study systematically any combination of surface buoyancy flux, buoyancy stratification, and wind shear; We refer to this dimensionless number as shear number. Seven simulations with shear numbers ranging from 0 (no wind) to 20 (moderate wind) are conducted; this range of shear numbers corresponds to wind strength from 0 to 15 m/s in the free troposphere for typical midday atmospheric conditions. In general, we find that shear effects are negligibly small when the shear number is below 10, and for larger values the effects remain constrained inside the entrainment zone and surface layer. This critical shear number is justified by scrutinizing the turbulence regimes (convective and mechanical) within the entrainment zone in the sense that, for this shear number, the turbulence transport of turbulence kinetic energy inside the entrainment zone equals the shear-production rate. Following this analysis a critical flux Richardson number of 0.6 inside the entrainment zone is found. In particular, we observe the following: First, the mean buoyancy and total buoyancy flux inside the mixed layer remain invariant under a change of shear number and they follow the free-convection scaling laws. Second, the height of minimum buoyancy flux increases due to shear effects, but just moderately (less than 5%). Nevertheless, this increment represents a growth of entrainment zone's thickness by 50% for shear numbers of the order of 20. Third, we observe that for shear numbers larger than 10, the entrainment flux ratio grows by up to 50% in an early state of ABL development. We provide explicit parameterizations of all these shear effects.

Dynamics of shearing force and its correlations with chemical compositions and in vitro dry matter digestibility of stylo (Stylosanthes guianensis) stem.

PubMed

Zi, Xuejuan; Li, Mao; Zhou, Hanlin; Tang, Jun; Cai, Yimin

2017-12-01

The study explored the dynamics of shearing force and its correlation with chemical compositions and in vitro dry matter digestibility (IVDMD) of stylo. The shearing force, diameter, linear density, chemical composition, and IVDMD of different height stylo stem were investigated. Linear regression analysis was done to determine the relationships between the shearing force and cut height, diameter, chemical composition, or IVDMD. The results showed that shearing force of stylo stem increased with plant height increasing and the crude protein (CP) content and IVDMD decreased but fiber content increased over time, resulting in decreased forage value. In addition, tall stem had greater shearing force than short stem. Moreover, shearing force is positively correlated with stem diameter, linear density and fiber fraction, but negatively correlated with CP content and IVDMD. Overall, shearing force is an indicator more direct, easier and faster to measure than chemical composition and digestibility for evaluation of forage nutritive value related to animal performance. Therefore, it can be used to evaluate the nutritive value of stylo.

Observations of Near-Surface Current Shear Help Describe Oceanic Oil and Plastic Transport

NASA Astrophysics Data System (ADS)

Laxague, Nathan J. M.; Ö-zgökmen, Tamay M.; Haus, Brian K.; Novelli, Guillaume; Shcherbina, Andrey; Sutherland, Peter; Guigand, Cédric M.; Lund, Björn; Mehta, Sanchit; Alday, Matias; Molemaker, Jeroen

2018-01-01

Plastics and spilled oil pose a critical threat to marine life and human health. As a result of wind forcing and wave motions, theoretical and laboratory studies predict very strong velocity variation with depth over the upper few centimeters of the water column, an observational blind spot in the real ocean. Here we present the first-ever ocean measurements of the current vector profile defined to within 1 cm of the free surface. In our illustrative example, the current magnitude averaged over the upper 1 cm of the ocean is shown to be nearly four times the average over the upper 10 m, even for mild forcing. Our findings indicate that this shear will rapidly separate pieces of marine debris which vary in size or buoyancy, making consideration of these dynamics essential to an improved understanding of the pathways along which marine plastics and oil are transported.

Mapping intramuscular tenderness variation in four major muscles of the beef round.

PubMed

Reuter, B J; Wulf, D M; Maddock, R J

2002-10-01

The objective of this study was to quantify intramuscular tenderness variation within four muscles from the beef round: biceps femoris (BF), semitendinosus (ST), semimembranosus (SM), and adductor (AD). At 48 h postmortem, the BF, ST, SM, and AD were dissected from either the left or right side of ten carcasses, vacuum packaged, and aged for an additional 8 d. Each muscle was then frozen and cut into 2.54-cm-thick steaks perpendicular to the long axis of the muscle. Steaks were broiled on electric broilers to an internal temperature of 71 degrees C. Location-specific cores were obtained from each cooked steak, and Warner-Bratzler shear force was evaluated. Definable intramuscular shear force variation (SD = 0.56 kg) was almost twice as large as between-animal shear force variation (SD = 0.29 kg) and 2.8 times as large as between-muscle variation (SD = 0.20 kg). The ranking of muscles from greatest to least definable intramuscular shear force variation was BF, SM, ST, and AD (SD = 1.09, 0.72, 0.29, and 0.15 kg, respectively). The BF had its lowest shear force values at the origin (sirloin end), intermediate shear force values at the insertion, and its highest shear force values in a middle region 7 to 10 cm posterior to the sirloin-round break point (P < 0.05). The BF had lower shear force values toward the ST side than toward the vastus lateralis side (P < 0.05). The ST had its lowest shear force values in a 10-cm region in the middle, and its highest shear force values toward each end (P < 0.05). The SM had its lowest shear force values in the first 10-cm from the ischial end (origin), and its highest shear force values in a 13-cm region at the insertion end (P < 0.05). Generally, shear force was lower toward the superficial (medial) side than toward the deep side of the SM (P < 0.05). There were no intramuscular differences in shear force values within the AD (P > 0.05). These data indicate that definable intramuscular tenderness variation is substantial and could be used to develop alternative fabrication and(or) merchandising methods for beef round muscles.

A finite element evaluation of the moment arm hypothesis for altered vertebral shear failure force.

PubMed

Howarth, Samuel J; Karakolis, Thomas; Callaghan, Jack P

2015-01-01

The mechanism of vertebral shear failure is likely a bending moment generated about the pars interarticularis by facet contact, and the moment arm length (MAL) between the centroid of facet contact and the location of pars interarticularis failure has been hypothesised to be an influential modulator of shear failure force. To quantitatively evaluate this hypothesis, anterior shear of C3 over C4 was simulated in a finite element model of the porcine C3-C4 vertebral joint with each combination of five compressive force magnitudes (0-60% of estimated compressive failure force) and three postures (flexed, neutral and extended). Bilateral locations of peak stress within C3's pars interarticularis were identified along with the centroids of contact force on the inferior facets. These measurements were used to calculate the MAL of facet contact force. Changes in MAL were also related to shear failure forces measured from similar in vitro tests. Flexed and extended vertebral postures respectively increased and decreased the MAL by 6.6% and 4.8%. The MAL decreased by only 2.6% from the smallest to the largest compressive force. Furthermore, altered MAL explained 70% of the variance in measured shear failure force from comparable in vitro testing with larger MALs being associated with lower shear failure forces. Our results confirmed that the MAL is indeed a significant modulator of vertebral shear failure force. Considering spine flexion is necessary when assessing low-back shear injury potential because of the association between altered facet articulation and lower vertebral shear failure tolerance.

Hydration and distance dependence of intermolecular shearing between collagen molecules in a model microfibril.

PubMed

Gautieri, Alfonso; Pate, Monica I; Vesentini, Simone; Redaelli, Alberto; Buehler, Markus J

2012-08-09

In vertebrates, collagen tissues are the main component responsible for force transmission. In spite of the physiological importance of these phenomena, force transmission mechanisms are still not fully understood, especially at smaller scales, including in particular collagen molecules and fibrils. Here we investigate the mechanism of molecular sliding between collagen molecules within a fibril, by shearing a central molecule in a hexagonally packed bundle mimicking the collagen microfibril environment, using varied lateral distance between the molecules in both dry and solvated conditions. In vacuum, the central molecule slides under a stick-slip mechanism that is due to the characteristic surface profile of collagen molecules, enhanced by the breaking and reformation of H-bonds between neighboring collagen molecules. This mechanism is consistently observed for varied lateral separations between molecules. The high shearing force (>7 nN) found for the experimentally observed intermolecular distance (≈1.1 nm) suggests that in dry samples the fibril elongation mechanism relies almost exclusively on molecular stretching, which may explain the higher stiffnesses found in dry fibrils. When hydrated, the slip-stick behavior is observed only below 1.3 nm of lateral distance, whereas above 1.3 nm the molecule shears smoothly, showing that the water layer has a strong lubricating effect. Moreover, the average force required to shear is approximately the same in solvated as in dry conditions (≈2.5 nN), which suggests that the role of water at the intermolecular level includes the transfer of load between molecules. Copyright © 2012 Elsevier Ltd. All rights reserved.

A novel capsulorhexis technique using shearing forces with cystotome.

PubMed

Karim, Shah M R; Ong, Chin T; Sleep, Tamsin J

2010-05-15

To demonstrate a capsulorhexis technique using predominantly shearing forces with a cystotome on a virtual reality simulator and on a human eye. Our technique involves creating the initial anterior capsular tear with a cystotome to raise a flap. The flap left unfolded on the lens surface. The cystotome tip is tilted horizontally and is engaged on the flap near the leading edge of the tear. The cystotome is moved in a circular fashion to direct the vector forces. The loose flap is constantly swept towards the centre so that it does not obscure the view on the tearing edge. Our technique has the advantage of reducing corneal wound distortion and subsequent anterior chamber collapse. The capsulorhexis flap is moved away from the tear leading edge allowing better visualisation of the direction of tear. This technique offers superior control of the capsulorhexis by allowing the surgeon to change the direction of the tear to achieve the desired capsulorhexis size. The EYESI Surgical Simulator is a realistic training platform for surgeons to practice complex capsulorhexis techniques. The shearing forces technique is a suitable alternative and in some cases a far better technique in achieving the desired capsulorhexis.

The Plunge Phase of Friction Stir Welding

NASA Technical Reports Server (NTRS)

Nunes, Arthur; McClure, John; Avila, Ricardo

2005-01-01

Torque and plunge force during the initial plunge phase in Friction Stir Welding were measured for a 0.5 inch diameter pin entering a 2219 aluminum alloy plate. Weld structures were preserved for metallographic observation by making emergency stops at various plunge depths. The plunging pin tool is seen to be surrounded by a very fine grained layer of recrystallized metal extending substantially below the bottom of the pin, implying a shear interface in the metal below and not at the tool-metal interface. Torque and plunge force during the initial plunge phase in Friction Stir Welding are calculated from a straight forward model based on a concept to plastic flow in the vicinity of the plunging tool compatible with structural observations. The concept: a disk of weld metal seized to and rotating with the bottom of the pin is squeezed out laterally by the plunge force and extruded upwards in a hollow cylinder around the tool. As the shear surface separating rotating disk from stationary weld metal engulfs fresh metal, the fresh metal is subjected to severe shear deformation, which results in its recrystallization. Encouraging agreement between computations and measured torque and plunge force is obtained.

The response of dense dry granular material to the shear reversal

NASA Astrophysics Data System (ADS)

Zhang, Jie; Ren, Jie; Farhadi, Somayeh; Behringer, Robert

2008-11-01

We have performed two dimensional granular experiments under pure shear using bidisperse photo-elastic disks. Starting from a stress free state, a square box filled with granular particles is subject to shear. The forward shears involved various number of steps, leading to maximum strains between 0.1 and 0.3. The area is kept constant during the shear. The network of force chains gradually built up as the strain increased, leading to increased pressure and shear stress. Reverse shear was then applied to the system. Depending on the initial packing fraction and the strain at which the shear is reversed, the force chain network built prior to the shear reversal may be destroyed completely or partially destroyed. Following the force chain weakening, when the reserve shear is continuously applied to the system, there is a force chain strengthening. Following each change of the system, contact forces of individual disks were measured by applying an inverse algorithm. We also kept track of the displacement and angle of rotation of every particle from frame to frame. We present the results for the structure failure and reconstruction during shear reversals. We also present data for stresses, contact force distributions and other statistical measures.

Ultrashort pulse laser micro-welding of cyclo-olefin copolymers

NASA Astrophysics Data System (ADS)

Roth, Gian-Luca; Rung, Stefan; Hellmann, Ralf

2017-06-01

We report on the joining of transparent thermoplastic polymers using infrared femtosecond laser pulses. Due to nonlinear absorption, the developed micro-welding process for cyclo-olefin copolymers does not require any intermediate absorbing layers or any surface pre-processing of the welding partners. In view of an optimized and stable micro-welding process, the influence of the welding speed and focal position on both, the quality and shear force strength are investigated. We highlight that welding seam widths of down to 65 μm are feasible for welding speeds of up to 75 mm/s. However, a variation of the welding speed affects the required focal position for a successful joining process. The shear force strength of the welding seam is determined to 37 MPa, which corresponds to 64% of the shear strength of the bulk material and is not affected by the welding speed.

Sinuous flow in metals

PubMed Central

Yeung, Ho; Viswanathan, Koushik; Compton, Walter Dale; Chandrasekar, Srinivasan

2015-01-01

Annealed metals are surprisingly difficult to cut, involving high forces and an unusually thick “chip.” This anomaly has long been explained, based on ex situ observations, using a model of smooth plastic flow with uniform shear to describe material removal by chip formation. Here we show that this phenomenon is actually the result of a fundamentally different collective deformation mode—sinuous flow. Using in situ imaging, we find that chip formation occurs via large-amplitude folding, triggered by surface undulations of a characteristic size. The resulting fold patterns resemble those observed in geophysics and complex fluids. Our observations establish sinuous flow as another mesoscopic deformation mode, alongside mechanisms such as kinking and shear banding. Additionally, by suppressing the triggering surface undulations, sinuous flow can be eliminated, resulting in a drastic reduction of cutting forces. We demonstrate this suppression quite simply by the application of common marking ink on the free surface of the workpiece material before the cutting. Alternatively, prehardening a thin surface layer of the workpiece material shows similar results. Besides obvious implications to industrial machining and surface generation processes, our results also help unify a number of disparate observations in the cutting of metals, including the so-called Rehbinder effect. PMID:26216980

Sinuous flow in metals.

PubMed

Yeung, Ho; Viswanathan, Koushik; Compton, Walter Dale; Chandrasekar, Srinivasan

2015-08-11

Annealed metals are surprisingly difficult to cut, involving high forces and an unusually thick "chip." This anomaly has long been explained, based on ex situ observations, using a model of smooth plastic flow with uniform shear to describe material removal by chip formation. Here we show that this phenomenon is actually the result of a fundamentally different collective deformation mode--sinuous flow. Using in situ imaging, we find that chip formation occurs via large-amplitude folding, triggered by surface undulations of a characteristic size. The resulting fold patterns resemble those observed in geophysics and complex fluids. Our observations establish sinuous flow as another mesoscopic deformation mode, alongside mechanisms such as kinking and shear banding. Additionally, by suppressing the triggering surface undulations, sinuous flow can be eliminated, resulting in a drastic reduction of cutting forces. We demonstrate this suppression quite simply by the application of common marking ink on the free surface of the workpiece material before the cutting. Alternatively, prehardening a thin surface layer of the workpiece material shows similar results. Besides obvious implications to industrial machining and surface generation processes, our results also help unify a number of disparate observations in the cutting of metals, including the so-called Rehbinder effect.

Generation Mechanism of Work Hardened Surface Layer in Metal Cutting

NASA Astrophysics Data System (ADS)

Hikiji, Rikio; Kondo, Eiji; Kawagoishi, Norio; Arai, Minoru

Finish machining used to be carried out in grinding, but it is being replaced by cutting with very small undeformed chip thickness. In ultra precision process, the effects of the cutting conditions and the complicated factors on the machined surface integrity are the serious problems. In this research, work hardened surface layer was dealt with as an evaluation of the machined surface integrity and the effect of the mechanical factors on work hardening was investigated experimentally in orthogonal cutting. As a result, it was found that work hardened surface layer was affected not only by the shear angle varied under the cutting conditions and the thrust force of cutting resistance, but also by the thrust force acting point, the coefficient of the thrust force and the compressive stress equivalent to the bulk hardness. Furthermore, these mechanical factors acting on the depth of the work hardened surface layer were investigated with the calculation model.

Design, development and applications of novel techniques for studying surface mechanical properties

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa

1989-01-01

Research is reviewed for the adhesion, friction, and micromechanical properties of materials and examples of the results presented. The ceramic and metallic materials studied include silicon carbide, aluminum oxide, and iron-base amorphous alloys. The design and operation of a torsion balance adapted for study of adhesion from the Cavendish balance are discussed first. The pull-off force (adhesion) and shear force (friction) required to break the interfacial junctions between contacting surfaces of the materials were examined at various temperatures in a vacuum. The surface chemistry of the materials was analyzed by X-ray photoelectron spectroscopy. Properties and environmental conditions of the surface regions which affect adhesion and friction-such as surface segregation, composition, crystal structure, surface chemistry, and temperature were also studied.

A unified approach for determining the ultimate strength of RC members subjected to combined axial force, bending, shear and torsion

PubMed Central

Huang, Zhen

2017-01-01

This paper uses experimental investigation and theoretical derivation to study the unified failure mechanism and ultimate capacity model of reinforced concrete (RC) members under combined axial, bending, shear and torsion loading. Fifteen RC members are tested under different combinations of compressive axial force, bending, shear and torsion using experimental equipment designed by the authors. The failure mechanism and ultimate strength data for the four groups of tested RC members under different combined loading conditions are investigated and discussed in detail. The experimental research seeks to determine how the ultimate strength of RC members changes with changing combined loads. According to the experimental research, a unified theoretical model is established by determining the shape of the warped failure surface, assuming an appropriate stress distribution on the failure surface, and considering the equilibrium conditions. This unified failure model can be reasonably and systematically changed into well-known failure theories of concrete members under single or combined loading. The unified calculation model could be easily used in design applications with some assumptions and simplifications. Finally, the accuracy of this theoretical unified model is verified by comparisons with experimental results. PMID:28414777

Approximate Stokes Drift Profiles and their use in Ocean Modelling

NASA Astrophysics Data System (ADS)

Breivik, Oyvind; Bidlot, Jea-Raymond; Janssen, Peter A. E. M.; Mogensen, Kristian

2016-04-01

Deep-water approximations to the Stokes drift velocity profile are explored as alternatives to the monochromatic profile. The alternative profiles investigated rely on the same two quantities required for the monochromatic profile, viz the Stokes transport and the surface Stokes drift velocity. Comparisons against parametric spectra and profiles under wave spectra from the ERA-Interim reanalysis and buoy observations reveal much better agreement than the monochromatic profile even for complex sea states. That the profiles give a closer match and a more correct shear has implications for ocean circulation models since the Coriolis-Stokes force depends on the magnitude and direction of the Stokes drift profile and Langmuir turbulence parameterizations depend sensitively on the shear of the profile. Of the two Stokes drift profiles explored here, the profile based on the Phillips spectrum is by far the best. In particular, the shear near the surface is almost identical to that influenced by the f-5 tail of spectral wave models. The NEMO general circulation ocean model was recently extended to incorporate the Stokes-Coriolis force along with two other wave-related effects. The ECWMF coupled atmosphere-wave-ocean ensemble forecast system now includes these wave effects in the ocean model component (NEMO).

Investigating the Mechanical Properties of Plasma von Willebrand Factor Using Atomic Force Microscopy

NASA Astrophysics Data System (ADS)

Wijeratne, Sitara; Botello, Eric; Yeh, Hui-Chun; Zhou, Zhou; Bergeron, Angela; Frey, Eric; Moake, Joel; Dong, Jing-Fei; Kiang, Ching-Hwa

2011-10-01

Single-molecule manipulation allows us to study the real-time kinetics of complex cellular processes. The mechanochemistry of different forms of von Willebrand factor (VWF) and their receptor-ligand binding kinetics can be probed by atomic force microscopy (AFM). Since plasma VWF can be activated upon shear, the structural and functional properties of VWF that are critical in mediating thrombus formation become important. Here we characterized the mechanical resistance to domain unfolding of VWF to determine its conformational states. We found the shear-induced conformational changes, hence the mechanical property, can be detected by the change in unfolding forces. The relaxation rate of such effect is much longer than expected. Our results offer an insight in establishing strategies for regulating VWF adhesion activity, increasing our understanding of surface-induced thrombosis as mediated by VWF.

Rubber friction: The contribution from the area of real contact.

PubMed

Tiwari, A; Miyashita, N; Espallargas, N; Persson, B N J

2018-06-14

There are two contributions to the friction force when a rubber block is sliding on a hard and rough substrate surface, namely, a contribution F ad = τ f A from the area of real contact A and a viscoelastic contribution F visc from the pulsating forces exerted by the substrate asperities on the rubber block. Here we present experimental results obtained at different sliding speeds and temperatures, and we show that the temperature dependency of the shear stress τ f , for temperatures above the rubber glass transition temperature T g , is weaker than that of the bulk viscoelastic modulus. The physical origin of τ f for T > T g is discussed, and we propose that its temperature dependency is determined by the rubber molecule segment mobility at the sliding interface, which is higher than in the bulk because of increased free-volume effect due to the short-wavelength surface roughness. This is consistent with the often observed reduction in the glass transition temperature in nanometer-thick surface layers of glassy polymers. For temperatures T < T g , the shear stress τ f is nearly velocity independent and of similar magnitude as observed for glassy polymers such as PMMA or polyethylene. In this case, the rubber undergoes plastic deformations in the asperity contact regions and the contact area is determined by the rubber penetration hardness. For this case, we propose that the frictional shear stress is due to slip at the interface between the rubber and a transfer film adsorbed on the concrete surface.

Rubber friction: The contribution from the area of real contact

NASA Astrophysics Data System (ADS)

Tiwari, A.; Miyashita, N.; Espallargas, N.; Persson, B. N. J.

2018-06-01

There are two contributions to the friction force when a rubber block is sliding on a hard and rough substrate surface, namely, a contribution Fad = τf A from the area of real contact A and a viscoelastic contribution Fvisc from the pulsating forces exerted by the substrate asperities on the rubber block. Here we present experimental results obtained at different sliding speeds and temperatures, and we show that the temperature dependency of the shear stress τf, for temperatures above the rubber glass transition temperature Tg, is weaker than that of the bulk viscoelastic modulus. The physical origin of τf for T > Tg is discussed, and we propose that its temperature dependency is determined by the rubber molecule segment mobility at the sliding interface, which is higher than in the bulk because of increased free-volume effect due to the short-wavelength surface roughness. This is consistent with the often observed reduction in the glass transition temperature in nanometer-thick surface layers of glassy polymers. For temperatures T < Tg, the shear stress τf is nearly velocity independent and of similar magnitude as observed for glassy polymers such as PMMA or polyethylene. In this case, the rubber undergoes plastic deformations in the asperity contact regions and the contact area is determined by the rubber penetration hardness. For this case, we propose that the frictional shear stress is due to slip at the interface between the rubber and a transfer film adsorbed on the concrete surface.

Understanding the destabilizing role for surface tension in planar shear flows in terms of wave interaction

NASA Astrophysics Data System (ADS)

Biancofiore, L.; Heifetz, E.; Hoepffner, J.; Gallaire, F.

2017-10-01

Both surface tension and buoyancy force in stable stratification act to restore perturbed interfaces back to their initial positions. Hence, both are intuitively considered as stabilizing agents. Nevertheless, the Taylor-Caulfield instability is a counterexample in which the presence of buoyancy forces in stable stratification destabilize shear flows. An explanation for this instability lies in the fact that stable stratification supports the existence of gravity waves. When two vertically separated gravity waves propagate horizontally against the shear, they may become phase locked and amplify each other to form a resonance instability. Surface tension is similar to buoyancy but its restoring mechanism is more efficient at small wavelengths. Here, we show how a modification of the Taylor-Caulfield configuration, including two interfaces between three stably stratified immiscible fluids, supports interfacial capillary gravity whose interaction yields resonance instability. Furthermore, when the three fluids have the same density, an instability arises solely due to a pure counterpropagating capillary wave resonance. The linear stability analysis predicts a maximum growth rate of the pure capillary wave instability for an intermediate value of surface tension corresponding to We-1=5 , where We denotes the Weber number. We perform direct numerical nonlinear simulation of this flow and find nonlinear destabilization when 2 ≤We-1≤10 , in good agreement with the linear stability analysis. The instability is present also when viscosity is introduced, although it is gradually damped and eventually quenched.

Subtidal circulation patterns in a shallow, highly stratified estuary: Mobile Bay, Alabama

USGS Publications Warehouse

Noble, M.A.; Schroeder, W.W.; Wiseman, W.J.; Ryan, H.F.; Gelfenbaum, G.

1996-01-01

Mobile Bay is a wide (25-50 km), shallow (3 m), highly stratified estuary on the Gulf coast of the United States. In May 1991 a series of instruments that measure near-surface and near-bed current, temperature, salinity, and middepth pressure were deployed for a year-long study of the bay. A full set of measurements were obtained at one site in the lower bay; all but current measurements were obtained at a midbay site. These observations show that the subtidal currents in the lower bay are highly sheared, despite the shallow depth of the estuary. The sheared flow patterns are partly caused by differential forcing from wind stress and river discharge. Two wind-driven flow patterns actually exist in lower Mobile Bay. A barotropic response develops when the difference between near-surface and near-bottom salinity is less than 5 parts per thousand. For stronger salinity gradients the wind-driven currents are larger and the response resembles a baroclinic flow pattern. Currents driven by river flows are sheared and also have a nonlinear response pattern. Only near-surface currents are driven seaward by discharges below 3000 m3/s. At higher discharge rates, surface current variability uncouples from the river flow and the increased discharge rates drive near-bed current seaward. This change in the river-forced flow pattern may be associated with a hydraulic jump in the mouth of the estuary. Copyright 1996 by the American Geophysical Union.

Simulation of shear thickening in attractive colloidal suspensions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pednekar, Sidhant; Chun, Jaehun; Morris, Jeffrey F.

2017-01-01

The influence of attractive forces between particles under conditions of large particle volume fraction is addressed using numerical simulations which account for hydrodynamic, Brownian, conservative and frictional contact forces. The focus is on conditions for which a significant increase in the apparent viscosity at small shear rates, and possibly the development of a yield stress, is observed. The high shear rate behavior for Brownian suspensions has been shown in recent work [R. Mari, R. Seto, J. F. Morris & M. M. Denn, PNAS, 2015, 112, 15326-15330] to be captured by the inclusion of pairwise forces of two forms, one amore » contact frictional interaction and the second a repulsive force common in stabilized colloidal dispersions. Under such conditions, shear thickening is observed when shear stress is comparable to the sum of the Brownian stress and a characteristic stress based on the combination of interparticle force with kT the thermal energy. At sufficiently large volume fraction, this shear thickening can be very abrupt. Here it is shown that when attractive interactions are present with the noted forces, the shear thickening is obscured, as the viscosity shear thins with increasing shear rate, eventually descending from an infinite value (yield stress conditions) to a plateau at large stress; this plateau is at the same level as the large-shear rate viscosity found in the shear thickened state without attractive forces. It is shown that this behavior is consistent with prior observations in shear thickening suspensions modified to be attractive through depletion flocculation [V. Gopalakrishnan & C. F. Zukoski J. Rheol., 2004, 48, 1321-1344]. The contributions of the contact, attractive, and hydrodynamics forces to the bulk stress are presented, as are the contact networks found at different attractive strengths.« less

Flow characterization of a spinner flask for induced pluripotent stem cell culture application.

PubMed

Ismadi, Mohd-Zulhilmi; Gupta, Priyanka; Fouras, Andreas; Verma, Paul; Jadhav, Sameer; Bellare, Jayesh; Hourigan, Kerry

2014-01-01

We present detailed quantitative measurement analyses for flow in a spinner flask with spinning rates between 20 to 45 RPM, utilizing the optical velocimetry measurement technique of Particle Image Velocimetry (PIV). A partial section of the impeller was immersed in the working fluid to reduce the shear forces induced on the cells cultured on microcarriers. Higher rotational speeds improved the mixing effect in the medium at the expense of a higher shear environment. It was found that the mouse induced pluripotent stem (iPS) cells achieved the optimum number of cells over 7 days in 25 RPM suspension culture. This condition translates to 0.0984 Pa of maximum shear stress caused by the interaction of the fluid flow with the bottom surface. However, inverse cell growth was obtained at 28 RPM culture condition. Such a narrow margin demonstrated that mouse iPS cells cultured on microcarriers are very sensitive to mechanical forces. This study provides insight to biomechanical parameters, specifically the shear stress distribution, for a commercially available spinner flask over a wide range of Reynolds number.

Performance et caracterisation a l'arrachement des tiges en FRP de carbone utilisees pour renforcement au cisaillement par la methode ETS

NASA Astrophysics Data System (ADS)

Ammar Khodja, L'Hady

The rehabilitation and strengthening concrete structures in shear using composite materials such as externally bonded (EB) or near surface mounted rebar (NSMR) are well established techniques. However, debonding of these strengthening materials is still present and constitute the principal cause of shear failure of beams strengthened with composite materials. A new method called ETS (Embedded Through Section) was recently developed in order to avoid premature failures due to debonding of composite materials. The objective of this study is to highlight the importance and influence of important parameters on the behavior of CFRP bars anchorages subjected to pullout forces. These parameters are: concrete strength, anchorage length of CFRP bars, hole diameter in concrete, diameter of the bar and CFRP surface type (smooth versus sanded). Understanding the influence of these parameters on the relationship between the pullout force and the slip is paramount. This allows an accurate description of the behavior of all elements that contribute to the resistance of the CFRP bars pullout. A series of 25 specimens were subjected to pullout tests. The impact of these parameters on the pullout performance of CFRP rods is summarized in terms of failure mode, ultimate tensile strength and loading force slip relationship. The results of these investigations show that using the ETS method, failure of the anchors can be avoided by providing adequate anchorage length and concrete strength. The method provides greater confinement and thus leads to a substantial improvement in the performance of anchors. As a result, designers will be able to avoid failures that are due to debonding of anchors using thereby the full capabilities of reinforced beams strengthened in shear with EB FRP. Keywords: ETS method, shear, strengthening, anchor, slip, FRP, NSM.

Interfacial Shear Strength and Adhesive Behavior of Silk Ionomer Surfaces.

PubMed

Kim, Sunghan; Geryak, Ren D; Zhang, Shuaidi; Ma, Ruilong; Calabrese, Rossella; Kaplan, David L; Tsukruk, Vladimir V

2017-09-11

The interfacial shear strength between different layers in multilayered structures of layer-by-layer (LbL) microcapsules is a crucial mechanical property to ensure their robustness. In this work, we investigated the interfacial shear strength of modified silk fibroin ionomers utilized in LbL shells, an ionic-cationic pair with complementary ionic pairing, (SF)-poly-l-glutamic acid (Glu) and SF-poly-l-lysine (Lys), and a complementary pair with partially screened Coulombic interactions due to the presence of poly(ethylene glycol) (PEG) segments and SF-Glu/SF-Lys[PEG] pair. Shearing and adhesive behavior between these silk ionomer surfaces in the swollen state were probed at different spatial scales and pressure ranges by using functionalized atomic force microscopy (AFM) tips as well as functionalized colloidal probes. The results show that both approaches were consistent in analyzing the interfacial shear strength of LbL silk ionomers at different spatial scales from a nanoscale to a fraction of a micron. Surprisingly, the interfacial shear strength between SF-Glu and SF-Lys[PEG] pair with partially screened ionic pairing was greater than the interfacial shear strength of the SF-Glu and SF-Lys pair with a high density of complementary ionic groups. The difference in interfacial shear strength and adhesive strength is suggested to be predominantly facilitated by the interlayer hydrogen bonding of complementary amino acids and overlap of highly swollen PEG segments.

Roughness-dependent tribology effects on discontinuous shear thickening

PubMed Central

Hsu, Chiao-Peng; Ramakrishna, Shivaprakash N.; Zanini, Michele; Spencer, Nicholas D.

2018-01-01

Surface roughness affects many properties of colloids, from depletion and capillary interactions to their dispersibility and use as emulsion stabilizers. It also impacts particle–particle frictional contacts, which have recently emerged as being responsible for the discontinuous shear thickening (DST) of dense suspensions. Tribological properties of these contacts have been rarely experimentally accessed, especially for nonspherical particles. Here, we systematically tackle the effect of nanoscale surface roughness by producing a library of all-silica, raspberry-like colloids and linking their rheology to their tribology. Rougher surfaces lead to a significant anticipation of DST onset, in terms of both shear rate and solid loading. Strikingly, they also eliminate continuous thickening. DST is here due to the interlocking of asperities, which we have identified as “stick–slip” frictional contacts by measuring the sliding of the same particles via lateral force microscopy (LFM). Direct measurements of particle–particle friction therefore highlight the value of an engineering-tribology approach to tuning the thickening of suspensions. PMID:29717043

Roughness-dependent tribology effects on discontinuous shear thickening.

PubMed

Hsu, Chiao-Peng; Ramakrishna, Shivaprakash N; Zanini, Michele; Spencer, Nicholas D; Isa, Lucio

2018-05-15

Surface roughness affects many properties of colloids, from depletion and capillary interactions to their dispersibility and use as emulsion stabilizers. It also impacts particle-particle frictional contacts, which have recently emerged as being responsible for the discontinuous shear thickening (DST) of dense suspensions. Tribological properties of these contacts have been rarely experimentally accessed, especially for nonspherical particles. Here, we systematically tackle the effect of nanoscale surface roughness by producing a library of all-silica, raspberry-like colloids and linking their rheology to their tribology. Rougher surfaces lead to a significant anticipation of DST onset, in terms of both shear rate and solid loading. Strikingly, they also eliminate continuous thickening. DST is here due to the interlocking of asperities, which we have identified as "stick-slip" frictional contacts by measuring the sliding of the same particles via lateral force microscopy (LFM). Direct measurements of particle-particle friction therefore highlight the value of an engineering-tribology approach to tuning the thickening of suspensions. Copyright © 2018 the Author(s). Published by PNAS.

Analysis of cell flux in the parallel plate flow chamber: implications for cell capture studies.

PubMed Central

Munn, L L; Melder, R J; Jain, R K

1994-01-01

The parallel plate flow chamber provides a controlled environment for determinations of the shear stress at which cells in suspension can bind to endothelial cell monolayers. By decreasing the flow rate of cell-containing media over the monolayer and assessing the number of cells bound at each wall shear stress, the relationship between shear force and binding efficiency can be determined. The rate of binding should depend on the delivery of cells to the surface as well as the intrinsic cell-surface interactions; thus, only if the cell flux to the surface is known can the resulting binding curves be interpreted correctly. We present the development and validation of a mathematical model based on the sedimentation rate and velocity profile in the chamber for the delivery of cells from a flowing suspension to the chamber surface. Our results show that the flux depends on the bulk cell concentration, the distance from the entrance point, and the flow rate of the cell-containing medium. The model was then used in a normalization procedure for experiments in which T cells attach to TNF-alpha-stimulated HUVEC monolayers, showing that a threshold for adhesion occurs at a shear stress of about 3 dyn/cm2. Images FIGURE 1 FIGURE 2 PMID:7948702

On the question of whether lubricants fluidize in stick–slip friction

PubMed Central

Rosenhek-Goldian, Irit; Kampf, Nir; Yeredor, Arie; Klein, Jacob

2015-01-01

Intermittent sliding (stick–slip motion) between solids is commonplace (e.g., squeaking hinges), even in the presence of lubricants, and is believed to occur by shear-induced fluidization of the lubricant film (slip), followed by its resolidification (stick). Using a surface force balance, we measure how the thickness of molecularly thin, model lubricant films (octamethylcyclotetrasiloxane) varies in stick–slip sliding between atomically smooth surfaces during the fleeting (ca. 20 ms) individual slip events. Shear fluidization of a film of five to six molecular layers during an individual slip event should result in film dilation of 0.4–0.5 nm, but our results show that, within our resolution of ca. 0.1 nm, slip of the surfaces is not correlated with any dilation of the intersurface gap. This reveals that, unlike what is commonly supposed, slip does not occur by such shear melting, and indicates that other mechanisms, such as intralayer slip within the lubricant film, or at its interface with the confining surfaces, may be the dominant dissipation modes. PMID:26039993

Rate Dependence in Force Networks of Sheared Granular Materials

NASA Astrophysics Data System (ADS)

Hartley, Robert; Behringer, Robert P.

2003-03-01

We describe experiments that explore rate dependence in force networks of dense granular materials undergoing slow deformation by shear and by compression. The experiments were carried out using 2D photoelastic particles so that it was possible to visualize forces at the grain scale. Shear experiments were carried out in a Couette geometry with a rate Ω. Compression experiments were carried out by repetitive compaction via a piston in a rigid chamber at comparable rates to the shear experiments. Under shearing the mean stress/force grew logarithmically with Ω for at least four decades. For compression, no dependence of the mean stress on rate was observed. In related measurements, we observed relaxation of stress in static samples that had been sheared and where the shearing was abruptly stopped. Relaxation of the force network occured over time scales of days. No relaxation of the force network was observable for uniformly compressed static samples. These results are of particular interest because they provide insight into creep and failure in granular materials.

Simulation of shear thickening in attractive colloidal suspensions.

PubMed

Pednekar, Sidhant; Chun, Jaehun; Morris, Jeffrey F

2017-03-01

The influence of attractive forces between particles under conditions of large particle volume fraction, ϕ, is addressed using numerical simulations which account for hydrodynamic, Brownian, conservative and frictional contact forces. The focus is on conditions for which a significant increase in the apparent viscosity at small shear rates, and possibly the development of a yield stress, is observed. The high shear rate behavior for Brownian suspensions has been shown in recent work [R. Mari, R. Seto, J. F. Morris and M. M. Denn PNAS, 2015, 112, 15326-15330] to be captured by the inclusion of pairwise forces of two forms, one a contact frictional interaction and the second a repulsive force often found in stabilized colloidal dispersions. Under such conditions, shear thickening is observed when shear stress is comparable to the sum of the Brownian stress, kT/a 3 , and a characteristic stress based on the combination of interparticle force, i.e. σ ∼ F 0 /a 2 with kT the thermal energy, F 0 the repulsive force scale and a the particle radius. At sufficiently large ϕ, this shear thickening can be very abrupt. Here it is shown that when attractive interactions are present with the noted forces, the shear thickening is obscured, as the viscosity shear thins with increasing shear rate, eventually descending from an infinite value (yield stress conditions) to a plateau at large stress; this plateau is at the same level as the large-shear rate viscosity found in the shear thickened state without attractive forces. It is shown that this behavior is consistent with prior observations in shear thickening suspensions modified to be attractive through depletion flocculation [V. Gopalakrishnan and C. F. Zukoski J. Rheol., 2004, 48, 1321-1344]. The contributions of the contact, attractive, and hydrodynamics forces to the bulk stress are presented, as are the contact networks found at different attractive strengths.

Effect of Plasma Surface Finish on Wettability and Mechanical Properties of SAC305 Solder Joints

NASA Astrophysics Data System (ADS)

Kim, Kyoung-Ho; Koike, Junichi; Yoon, Jeong-Won; Yoo, Sehoon

2016-12-01

The wetting behavior, interfacial reactions, and mechanical reliability of Sn-Ag-Cu solder on a plasma-coated printed circuit board (PCB) substrate were evaluated under multiple heat-treatments. Conventional organic solderability preservative (OSP) finished PCBs were used as a reference. The plasma process created a dense and highly cross-linked polymer coating on the Cu substrates. The plasma finished samples had higher wetting forces and shorter zero-cross times than those with OSP surface finish. The OSP sample was degraded after sequential multiple heat treatments and reflow processes, whereas the solderability of the plasma finished sample was retained after multiple heat treatments. After the soldering process, similar microstructures were observed at the interfaces of the two solder joints, where the development of intermetallic compounds was observed. From ball shear tests, it was found that the shear force for the plasma substrate was consistently higher than that for the OSP substrate. Deterioration of the OSP surface finish was observed after multiple heat treatments. Overall, the plasma surface finish was superior to the conventional OSP finish with respect to wettability and joint reliability, indicating that it is a suitable material for the fabrication of complex electronic devices.

Single-cell force spectroscopy of pili-mediated adhesion

NASA Astrophysics Data System (ADS)

Sullan, Ruby May A.; Beaussart, Audrey; Tripathi, Prachi; Derclaye, Sylvie; El-Kirat-Chatel, Sofiane; Li, James K.; Schneider, Yves-Jacques; Vanderleyden, Jos; Lebeer, Sarah; Dufrêne, Yves F.

2013-12-01

Although bacterial pili are known to mediate cell adhesion to a variety of substrates, the molecular interactions behind this process are poorly understood. We report the direct measurement of the forces guiding pili-mediated adhesion, focusing on the medically important probiotic bacterium Lactobacillus rhamnosus GG (LGG). Using non-invasive single-cell force spectroscopy (SCFS), we quantify the adhesion forces between individual bacteria and biotic (mucin, intestinal cells) or abiotic (hydrophobic monolayers) surfaces. On hydrophobic surfaces, bacterial pili strengthen adhesion through remarkable nanospring properties, which - presumably - enable the bacteria to resist high shear forces under physiological conditions. On mucin, nanosprings are more frequent and adhesion forces larger, reflecting the influence of specific pili-mucin bonds. Interestingly, these mechanical responses are no longer observed on human intestinal Caco-2 cells. Rather, force curves exhibit constant force plateaus with extended ruptures reflecting the extraction of membrane nanotethers. These single-cell analyses provide novel insights into the molecular mechanisms by which piliated bacteria colonize surfaces (nanosprings, nanotethers), and offer exciting avenues in nanomedicine for understanding and controlling the adhesion of microbial cells (probiotics, pathogens).

A microfabricated gecko-inspired controllable and reusable dry adhesive

NASA Astrophysics Data System (ADS)

Chary, Sathya; Tamelier, John; Turner, Kimberly

2013-02-01

Geckos utilize a robust reversible adhesive to repeatedly attach and detach from a variety of vertical and inverted surfaces, using structurally anisotropic micro- and nano-scale fibrillar structures. These fibers, when suitably articulated, are able to control the real area of contact and thereby generate high-to-low van der Waals forces. Key characteristics of the natural system include highly anisotropic adhesion and shear forces for controllable attachment, a high adhesion to initial preload force ratio (μ‧) of 8-16, lack of inter-fiber self-adhesion, and operation over more than 30 000 cycles without loss of adhesion performance. A highly reusable synthetic adhesive has been developed using tilted polydimethylsiloxane (PDMS) half-cylinder micron-scale fibers, retaining up to 77% of the initial value over 10 000 repeated test cycles against a flat glass puck. In comparison with other gecko-inspired adhesives tested over 10 000 cycles or more thus far, this paper reports the highest value of μ‧, along with a large shear force of ˜78 kPa, approaching the 88-226 kPa range of gecko toes. The anisotropic adhesion forces are close to theoretical estimates from the Kendall peel model, quantitatively showing how lateral shearing articulation in a manner similar to the gecko may be used to obtain adhesion anisotropy with synthetic fibers using a combination of tilt angle and anisotropic fiber geometry.

Various Numerical Applications on Tropical Convective Systems Using a Cloud Resolving Model

NASA Technical Reports Server (NTRS)

Shie, C.-L.; Tao, W.-K.; Simpson, J.

2003-01-01

In recent years, increasing attention has been given to cloud resolving models (CRMs or cloud ensemble models-CEMs) for their ability to simulate the radiative-convective system, which plays a significant role in determining the regional heat and moisture budgets in the Tropics. The growing popularity of CRM usage can be credited to its inclusion of crucial and physically relatively realistic features such as explicit cloud-scale dynamics, sophisticated microphysical processes, and explicit cloud-radiation interaction. On the other hand, impacts of the environmental conditions (for example, the large-scale wind fields, heat and moisture advections as well as sea surface temperature) on the convective system can also be plausibly investigated using the CRMs with imposed explicit forcing. In this paper, by basically using a Goddard Cumulus Ensemble (GCE) model, three different studies on tropical convective systems are briefly presented. Each of these studies serves a different goal as well as uses a different approach. In the first study, which uses more of an idealized approach, the respective impacts of the large-scale horizontal wind shear and surface fluxes on the modeled tropical quasi-equilibrium states of temperature and water vapor are examined. In this 2-D study, the imposed large-scale horizontal wind shear is ideally either nudged (wind shear maintained strong) or mixed (wind shear weakened), while the minimum surface wind speed used for computing surface fluxes varies among various numerical experiments. For the second study, a handful of real tropical episodes (TRMM Kwajalein Experiment - KWAJEX, 1999; TRMM South China Sea Monsoon Experiment - SCSMEX, 1998) have been simulated such that several major atmospheric characteristics such as the rainfall amount and its associated stratiform contribution, the Qlheat and Q2/moisture budgets are investigated. In this study, the observed large-scale heat and moisture advections are continuously applied to the 2-D model. The modeled cloud generated from such an approach is termed continuously forced convection or continuous large-scale forced convection. A third study, which focuses on the respective impact of atmospheric components on upper Ocean heat and salt budgets, will be presented in the end. Unlike the two previous 2-D studies, this study employs the 3-D GCE-simulated diabatic source terms (using TOGA COARE observations) - radiation (longwave and shortwave), surface fluxes (sensible and latent heat, and wind stress), and precipitation as input for the Ocean mixed-layer (OML) model.

Effects of surface pressure and internal friction on the dynamics of shear-driven supported lipid bilayers.

PubMed

Jönsson, Peter; Höök, Fredrik

2011-02-15

Supported lipid bilayers (SLBs) are one of the most common model systems for cell membrane studies. We have previously found that when applying a bulk flow of liquid above an SLB the lipid bilayer and its constituents move in the direction of the bulk flow in a rolling type of motion, with the lower monolayer being essentially stationary. In this study, a theoretical platform is developed to model the dynamic behavior of a shear-driven SLB. In most regions of the moving SLB, the dynamics of the lipid bilayer is well explained by a balance between the hydrodynamic shear force arising from the bulk flow above the lipid bilayer and the friction between the upper and lower monolayers of the SLB. These two forces result in a drift velocity profile for the lipids in the upper monolayer of the SLB that is highest at the center of the channel and decreases to almost zero at the corners of the channel. However, near the front of an advancing SLB a very different flow behavior is observed, showing an almost constant drift velocity of the lipids over the entire bilayer front. In this region, the motion of the SLB is significantly influenced by gradients in the surface pressure as well as internal friction due to molecules that have accumulated at the front of the SLB. It is shown that even a modest surface fraction of accumulated molecules (∼1%) can drastically affect the behavior of the SLB near the bilayer front, forcing the advancing lipids in the SLB away from the center of the channel out toward the sides.

Early Student Support for a Process Study of Oceanic Responses to Typhoons

DTIC Science & Technology

2015-06-21

responses to tropical cyclone forcing are surface waves, wind-driven currents, shear and turbulence, and inertial currents. Quantifying the effect ...Cd is estimated assuming a balance between the time rate change of the depth-integrated horizontal momentum, Coriolis force, and the wind stress. This...negligible pressure gradient effect . Most of the observed horizontal kinetic energy is within the upper 100 m. The available potential energy and

Fracture of a composite reinforced by unidirectional fibers

NASA Astrophysics Data System (ADS)

Hasanov, F. F.

2014-11-01

An elastic medium weakened by a periodic system of circular holes filled with homogeneous elastic fibers whose surface is coated with a homogeneous film is considered. A fracture model for a medium with a periodic structure is proposed, which is based on an analysis of the fracture zone near the crack tip. It is assumed that the fracture zone is a layer of finite length containing a material with partially broken bonds between separate structural elements (end zone). The fracture zone is considered as part of the crack. The bonds between crack faces in the end zone are modeled by applying the cohesive forces caused by the presence of bonds to the crack surface. An analysis of the limit equilibrium of shear cracks in the end zone of the model is performed on the basis of a nonlocal fracture criterion together with a force condition for the motion of crack tip and a deformation condition for determining the motion of faces of end-zone cracks. In the analysis, relationships between the cohesive forces and the shear of crack faces are established, the stress state near the crack is assessed with account of external loading, cohesive forces, and fiber arrangement, and the critical external loads as functions of geometric parameters of the composite are determined.

Influence of thickness and permeability of endothelial surface layer on transmission of shear stress in capillaries

NASA Astrophysics Data System (ADS)

Zhang, SongPeng; Zhang, XiangJun; Tian, Yu; Meng, YongGang; Lipowsky, Herbert

2015-07-01

The molecular coating on the surface of microvascular endothelium has been identified as a barrier to transvascular exchange of solutes. With a thickness of hundreds of nanometers, this endothelial surface layer (ESL) has been treated as a porous domain within which fluid shear stresses are dissipated and transmitted to the solid matrix to initiate mechanotransduction events. The present study aims to examine the effects of the ESL thickness and permeability on the transmission of shear stress throughout the ESL. Our results indicate that fluid shear stresses rapidly decrease to insignificant levels within a thin transition layer near the outer boundary of the ESL with a thickness on the order of ten nanometers. The thickness of the transition zone between free fluid and the porous layer was found to be proportional to the square root of the Darcy permeability. As the permeability is reduced ten-fold, the interfacial fluid and solid matrix shear stress gradients increase exponentially two-fold. While the interfacial fluid shear stress is positively related to the ESL thickness, the transmitted matrix stress is reduced by about 50% as the ESL thickness is decreased from 500 to 100 nm, which may occur under pathological conditions. Thus, thickness and permeability of the ESL are two main factors that determine flow features and the apportionment of shear stresses between the fluid and solid phases of the ESL. These results may shed light on the mechanisms of force transmission through the ESL and the pathological events caused by alterations in thickness and permeability of the ESL.

Influence of neighboring adherent cells on laminar flow induced shear stress in vitro—A systematic study

PubMed Central

Djukelic, Mario; Westerhausen, Christoph

2017-01-01

Cells experience forces if subjected to laminar flow. These forces, mostly of shear force character, are strongly dependent not only on the applied flow field itself but also on hydrodynamic effects originating from neighboring cells. This particularly becomes important for the interpretation of data from in vitro experiments in flow chambers without confluent cell layers. By employing numerical Finite Element Method simulations of such assemblies of deformable objects under shear flow, we investigate the occurring stress within elastic adherent cells and the influence of neighboring cells on these quantities. For this, we simulate single and multiple adherent cells of different shapes fixed on a solid substrate under laminar flow parallel to the substrate for different velocities. We determine the local stress within the cells close to the cell-substrate-interface and the overall stress of the cells by surface integration over the cell surface. Comparing each measurand in the case of a multiple cell situation with the corresponding one of single cells under identical conditions, we introduce a dimensionless influence factor. The systematic variation of the distance and angle between cells, where the latter is with respect to the flow direction, flow velocity, Young's modulus, cell shape, and cell number, enables us to describe the actual influence on a cell. Overall, we here demonstrate that the cell density is a crucial parameter for all studies on flow induced experiments on adherent cells in vitro. PMID:28798851

Functionally Different Pads on the Same Foot Allow Control of Attachment: Stick Insects Have Load-Sensitive “Heel” Pads for Friction and Shear-Sensitive “Toe” Pads for Adhesion

PubMed Central

Labonte, David; Federle, Walter

2013-01-01

Stick insects (Carausius morosus) have two distinct types of attachment pad per leg, tarsal “heel” pads (euplantulae) and a pre-tarsal “toe” pad (arolium). Here we show that these two pad types are specialised for fundamentally different functions. When standing upright, stick insects rested on their proximal euplantulae, while arolia were the only pads in surface contact when hanging upside down. Single-pad force measurements showed that the adhesion of euplantulae was extremely small, but friction forces strongly increased with normal load and coefficients of friction were 1. The pre-tarsal arolium, in contrast, generated adhesion that strongly increased with pulling forces, allowing adhesion to be activated and deactivated by shear forces, which can be produced actively, or passively as a result of the insects' sprawled posture. The shear-sensitivity of the arolium was present even when corrected for contact area, and was independent of normal preloads covering nearly an order of magnitude. Attachment of both heel and toe pads is thus activated partly by the forces that arise passively in the situations in which they are used by the insects, ensuring safe attachment. Our results suggest that stick insect euplantulae are specialised “friction pads” that produce traction when pressed against the substrate, while arolia are “true” adhesive pads that stick to the substrate when activated by pulling forces. PMID:24349156

A numerical analysis of forces exerted by laminar flow on spreading cells in a parallel plate flow chamber assay.

PubMed

Olivier, L A; Truskey, G A

1993-10-01

Exposure of spreading anchorage-dependent cells to laminar flow is a common technique to measure the strength of cell adhesion. Since cells protrude into the flow stream, the force exerted by the fluid on the cells is a function of cell shape. To assess the relationship between cell shape and the hydrodynamic force on adherent cells, we obtained numerical solutions of the velocity and stress fields around bovine aortic endothelial cells during various stages of spreading and calculated the force required to detach the cells. Morphometric parameters were obtained from light and scanning electron microscopy measurements. Cells were assumed to have a constant volume, but the surface area increased during spreading until the membrane was stretched taut. Two-dimensional models of steady flow were generated using the software packages ANSYS (mesh generation) and FIDAP (problem solution). The validity of the numerical results was tested by comparison with published results for a semicircle in contact with the surface. The drag force and torque were greatest for round cells making initial contact with the surface. During spreading, the drag force and torque declined by factors of 2 and 20, respectively. The calculated forces and moments were used in adhesion models to predict the wall shear stress at which the cells detached. Based upon published values for the bond force and receptor number, round cells should detach at shear stresses between 2.5 and 6 dyn/cm(2), whereas substantially higher stresses are needed to detach spreading and fully spread cells. Results from the simulations indicate that (1) the drag force varies little with cell shape whereas the torque is very sensitive to cell shape, and (2) the increase in the strength of adhesion during spreading is due to increased contact area and receptor densities within the contact area. (c) 1993 John Wiley & Sons, Inc.

Chirality-specific lift forces of helix under shear flows: Helix perpendicular to shear plane.

PubMed

Zhang, Qi-Yi

2017-02-01

Chiral objects in shear flow experience a chirality-specific lift force. Shear flows past helices in a low Reynolds number regime were studied using slender-body theory. The chirality-specific lift forces in the vorticity direction experienced by helices are dominated by a set of helix geometry parameters: helix radius, pitch length, number of turns, and helix phase angle. Its analytical formula is given. The chirality-specific forces are the physical reasons for the chiral separation of helices in shear flow. Our results are well supported by the latest experimental observations. © 2016 Wiley Periodicals, Inc.

Synergistic Interactions of a Synthetic Lubricin-Mimetic with Fibronectin for Enhanced Wear Protection

PubMed Central

Andresen Eguiluz, Roberto C.; Cook, Sierra G.; Tan, Mingchee; Brown, Cory N.; Pacifici, Noah J.; Samak, Mihir S.; Bonassar, Lawrence J.; Putnam, David; Gourdon, Delphine

2017-01-01

Lubricin (LUB), a major mucinous glycoprotein of mammalian synovial fluids, is believed to provide excellent lubrication to cartilage surfaces. Consequently, when joint disease or replacement leads to increased friction and surface damage in the joint, robust synthetic LUB alternatives that could be used therapeutically to improve lubrication and surface protection are needed. Here, we report the characterization of a lubricating multiblock bottlebrush polymer whose architecture was inspired by LUB, and we investigate the role of fibronectin (FN), a glycoprotein found in the superficial zone of cartilage, in mediating the tribological properties of the polymer upon shear between mica surfaces. Our surface forces apparatus (SFA) normal force measurements indicate that the lubricin-mimetic (mimLUB) could be kept anchored between mica surfaces, even under high contact pressures, when an intermediate layer of FN was present. Additional SFA friction measurements show that FN would also extend the wearless friction regime of the polymer up to pressures of 3.4 MPa while ensuring stable friction coefficients (μ ≈ 0.28). These results demonstrate synergistic interactions between mimLUB and FN in assisting the lubrication and wear protection of ideal (mica) substrates upon shear. Collectively, these findings suggest that our proposed mimLUB might be a promising alternative to LUB, as similar mechanisms could potentially facilitate the interaction between the polymer and cartilage surfaces in articular joints and prosthetic implants in vivo. PMID:28702455

Dependence of nanomechanical modification of polymers on plasma-induced cross-linking

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tajima, S.; Komvopoulos, K.

2007-01-01

The nanomechanical properties of low-density polyethylene (LDPE) modified by inductively coupled, radio-frequency Ar plasma were investigated by surface force microscopy. The polymer surface was modified under plasma conditions of different ion energy fluences and radiation intensities obtained by varying the sample distance from the plasma power source. Nanoindentation results of the surface stiffness versus maximum penetration depth did not reveal discernible differences between untreated and plasma-treated LDPE, presumably due to the small thickness of the modified surface layer that resulted in a substrate effect. On the contrary, nanoscratching experiments demonstrated a significant increase in the surface shear resistance of plasma-modifiedmore » LDPE due to chain cross-linking. These experiments revealed an enhancement of cross-linking with increasing ion energy fluence and radiation intensity, and a tip size effect on the friction force and dominant friction mechanisms (adhesion, plowing, and microcutting). In addition, LDPE samples with a LiF crystal shield were exposed to identical plasma conditions to determine the role of vacuum ultraviolet (VUV) and ultraviolet (UV) radiation in the cross-linking process. The cross-linked layer of plasma-treated LDPE exhibited much higher shear strength than that of VUV/UV-treated LDPE. Plasma-induced surface modification of the nanomechanical properties of LDPE is interpreted in the context of molecular models of the untreated and cross-linked polymer surfaces derived from experimental findings.« less

In-flight boundary-layer measurements on a hollow cylinder at a Mach number of 3.0

NASA Technical Reports Server (NTRS)

Quinn, R. D.; Gong, L.

1980-01-01

Skin temperatures, shear forces, surface static pressures, boundary layer pitot pressures, and boundary layer total temperatures were measured on the external surface of a hollow cylinder that was 3.04 meters long and 0.437 meter in diameter and was mounted beneath the fuselage of the YF-12A airplane. The data were obtained at a nominal free stream Mach number of 3.0 (a local Mach number of 2.9) and at wall to recovery temperature ratios of 0.66 to 0.91. The local Reynolds number had a nominal value of 4,300,000 per meter. Heat transfer coefficients and skin friction coefficients were derived from skin temperature time histories and shear force measurements, respectively. In addition, boundary layer velocity profiles were derived from pitot pressure measurements, and a Reynolds analogy factor was obtained from the heat transfer and skin friction measurements. The measured data are compared with several boundary layer prediction methods.

Asymptotic stability of shear-flow solutions to incompressible viscous free boundary problems with and without surface tension

NASA Astrophysics Data System (ADS)

Tice, Ian

2018-04-01

This paper concerns the dynamics of a layer of incompressible viscous fluid lying above a rigid plane and with an upper boundary given by a free surface. The fluid is subject to a constant external force with a horizontal component, which arises in modeling the motion of such a fluid down an inclined plane, after a coordinate change. We consider the problem both with and without surface tension for horizontally periodic flows. This problem gives rise to shear-flow equilibrium solutions, and the main thrust of this paper is to study the asymptotic stability of the equilibria in certain parameter regimes. We prove that there exists a parameter regime in which sufficiently small perturbations of the equilibrium at time t=0 give rise to global-in-time solutions that return to equilibrium exponentially in the case with surface tension and almost exponentially in the case without surface tension. We also establish a vanishing surface tension limit, which connects the solutions with and without surface tension.

A validated computational model for the design of surface textures in full-film lubricated sliding

NASA Astrophysics Data System (ADS)

Schuh, Jonathon; Lee, Yong Hoon; Allison, James; Ewoldt, Randy

2016-11-01

Our recent experimental work showed that asymmetry is needed for surface textures to decrease friction in full-film lubricated sliding (thrust bearings) with Newtonian fluids; textures reduce the shear load and produce a separating normal force. The sign of the separating normal force is not predicted by previous 1-D theories. Here we model the flow with the Reynolds equation in cylindrical coordinates, numerically implemented with a pseudo-spectral method. The model predictions match experiments, rationalize the sign of the normal force, and allow for design of surface texture geometry. To minimize sliding friction with angled cylindrical textures, an optimal angle of asymmetry β exists. The optimal angle depends on the film thickness but not the sliding velocity within the applicable range of the model. The model has also been used to optimize generalized surface texture topography while satisfying manufacturability constraints.

Sinusoidal Forcing of Interfacial Films

NASA Astrophysics Data System (ADS)

Rasheed, Fayaz; Raghunandan, Aditya; Hirsa, Amir; Lopez, Juan

2015-11-01

Fluid transport, in vivo, is accomplished via pumping mechanisms of the heart and lungs, which results in biological fluids being subjected to oscillatory shear. Flow is known to influence biological macromolecules, but predicting the effect of shear is incomplete without also accounting for the influence of complex interfaces ubiquitous throughout the body. Here, we investigated the oscillatory response of the structure of aqueous interfacial films using a cylindrical knife edge viscometer. Vitamin K1 was used as a model monolayer because its behaviour has been thoroughly quantified and it doesn't show any measurable hysteresis. The monolayer was subjected to sinusoidal forcing under varied conditions of surface concentrations, periodic frequencies, and knife edge amplitudes. Particle Image Velocimetry(PIV) data was collected using Brewster Angle Microscopy(BAM), revealing the influence of oscillatory interfacial shear stress on the monolayer. Insights were gained as to how the velocity profile dampens at specific distances from the knife edge contact depending on the amplitude, frequency, and concentration of Vitamin K1. Supported by NNX13AQ22G, National Aeronautics and Space Administration.

Dynamically adjustable foot-ground contact model to estimate ground reaction force during walking and running.

PubMed

Jung, Yihwan; Jung, Moonki; Ryu, Jiseon; Yoon, Sukhoon; Park, Sang-Kyoon; Koo, Seungbum

2016-03-01

Human dynamic models have been used to estimate joint kinetics during various activities. Kinetics estimation is in demand in sports and clinical applications where data on external forces, such as the ground reaction force (GRF), are not available. The purpose of this study was to estimate the GRF during gait by utilizing distance- and velocity-dependent force models between the foot and ground in an inverse-dynamics-based optimization. Ten males were tested as they walked at four different speeds on a force plate-embedded treadmill system. The full-GRF model whose foot-ground reaction elements were dynamically adjusted according to vertical displacement and anterior-posterior speed between the foot and ground was implemented in a full-body skeletal model. The model estimated the vertical and shear forces of the GRF from body kinematics. The shear-GRF model with dynamically adjustable shear reaction elements according to the input vertical force was also implemented in the foot of a full-body skeletal model. Shear forces of the GRF were estimated from body kinematics, vertical GRF, and center of pressure. The estimated full GRF had the lowest root mean square (RMS) errors at the slow walking speed (1.0m/s) with 4.2, 1.3, and 5.7% BW for anterior-posterior, medial-lateral, and vertical forces, respectively. The estimated shear forces were not significantly different between the full-GRF and shear-GRF models, but the RMS errors of the estimated knee joint kinetics were significantly lower for the shear-GRF model. Providing COP and vertical GRF with sensors, such as an insole-type pressure mat, can help estimate shear forces of the GRF and increase accuracy for estimation of joint kinetics. Copyright © 2016 Elsevier B.V. All rights reserved.

The RiSE climbing robot: body and leg design

NASA Astrophysics Data System (ADS)

Saunders, A.; Goldman, D. I.; Full, R. J.; Buehler, M.

2006-05-01

The RiSE robot is a biologically inspired, six legged climbing robot, designed for general mobility in scansorial (vertical walls, horizontal ledges, ground level) environments. It exhibits ground reaction forces that are similar to animal climbers and does not rely on suction, magnets or other surface-dependent specializations to achieve adhesion and shear force. We describe RiSE's body and leg design as well as its electromechanical, communications and computational infrastructure. We review design iterations that enable RiSE to climb 90° carpeted, cork covered and (a growing range of) stucco surfaces in the quasi-static regime.

Shear Moduli of Orthotropic Composites.

DTIC Science & Technology

1980-03-01

COMPOSITES N. J. Pagano Mechanics & Surface Interactions Branch Nonmetallic Materials Division March 1980 DT1C> Technical Report AFML-TR-79-4164 ELE(I JUN...Surface Interactions Br. Nonmetallic Materials Division Nonmetallic Materials Division FOR THE COMMANDER M.KLLChief metallic Materials Division "If your...SrtaceT’ Ileation ranch 10. PROGRAM ELEMENT. PROJECT, TASK Materials Laboratory 219 u,\\i’- Air Force Wright Aeronautical Laboratories 21310, WD Wright

Particle motion in atmospheric boundary layers of Mars and Earth

NASA Technical Reports Server (NTRS)

White, B. R.; Iversen, J. D.; Greeley, R.; Pollack, J. B.

1975-01-01

To study the eolian mechanics of saltating particles, both an experimental investigation of the flow field around a model crater in an atmospheric boundary layer wind tunnel and numerical solutions of the two- and three-dimensional equations of motion of a single particle under the influence of a turbulent boundary layer were conducted. Two-dimensional particle motion was calculated for flow near the surfaces of both Earth and Mars. For the case of Earth both a turbulent boundary layer with a viscous sublayer and one without were calculated. For the case of Mars it was only necessary to calculate turbulent boundary layer flow with a laminar sublayer because of the low values of friction Reynolds number; however, it was necessary to include the effects of slip flow on a particle caused by the rarefied Martian atmosphere. In the equations of motion the lift force functions were developed to act on a single particle only in the laminar sublayer or a corresponding small region of high shear near the surface for a fully turbulent boundary layer. The lift force functions were developed from the analytical work by Saffman concerning the lift force acting on a particle in simple shear flow.

Rapid tenderisation of lamb M. longissimus with very fast chilling depends on rapidly achieving sub-zero temperatures.

PubMed

Jacob, Robin; Rosenvold, Katja; North, Michael; Kemp, Robert; Warner, Robyn; Geesink, Geert

2012-09-01

A study was undertaken to determine whether variations within the defined temperature-by-time profile for very fast chilling (VFC), might explain variations in tenderness found with VFC. Loins from 32 lambs were subjected to one of five cooling regimes; defined by the average temperature between the meat surface and centre reached at a specific time post mortem. These were: -0.3 °C at 22 h (Control), 2.6 °C at 1.5 h (Fast(supra-zero)), 0.7 °C at 5.5 h (Slow(supra-zero)), -1.6 °C at 1.5 h (Fast(sub-zero)) and -2.3 °C at 5.5 h (Slow(sub-zero)), respectively. Shear force values considered very tender by consumers (less than 50 N, MIRINZ tenderometer) were found 2 days post mortem in Fast(sub-zero) loins only. Both time and temperature at the end of the cooling period contributed to variations in shear force. To achieve low shear force, the loins needed to be cooled to less than 0 °C at 1.5 h post mortem. Copyright © 2012 Elsevier Ltd. All rights reserved.

Biomechanics of ant adhesive pads: frictional forces are rate- and temperature-dependent.

PubMed

Federle, Walter; Baumgartner, Werner; Hölldobler, Bert

2004-01-01

Tarsal adhesive pads enable insects to hold on to smooth plant surfaces. Using a centrifuge technique, we tested whether a "wet adhesion" model of a thin film of liquid secreted between the pad and the surface can explain adhesive and frictional forces in Asian Weaver ants (Oecophylla smaragdina). When forces are acting parallel to the surface, pads in contact with the surface can slide smoothly. Force per unit pad contact area was strongly dependent on sliding velocity and temperature. Seemingly consistent with the effect of a thin liquid film in the contact zone, (1) frictional force linearly increased with sliding velocity, (2) the increment was greater at lower temperatures and (3) no temperature dependence was detected for low-rate perpendicular detachment forces. However, we observed a strong, temperature-independent static friction that was inconsistent with a fully lubricated contact. Static friction was too large to be explained by the contribution of other (sclerotized) body parts. Moreover, the rate-specific increase of shear stress strongly exceeded predictions derived from estimates of the adhesive liquid film's thickness and viscosity. Both lines of evidence indicate that the adhesive secretion alone is insufficient to explain the observed forces and that direct interaction of the soft pad cuticle with the surface ("rubber friction") is involved.

Atomic Force Microscope Mediated Chromatography

NASA Technical Reports Server (NTRS)

Anderson, Mark S.

2013-01-01

The atomic force microscope (AFM) is used to inject a sample, provide shear-driven liquid flow over a functionalized substrate, and detect separated components. This is demonstrated using lipophilic dyes and normal phase chromatography. A significant reduction in both size and separation time scales is achieved with a 25-micron-length column scale, and one-second separation times. The approach has general applications to trace chemical and microfluidic analysis. The AFM is now a common tool for ultra-microscopy and nanotechnology. It has also been demonstrated to provide a number of microfluidic functions necessary for miniaturized chromatography. These include injection of sub-femtoliter samples, fluidic switching, and sheardriven pumping. The AFM probe tip can be used to selectively remove surface layers for subsequent microchemical analysis using infrared and tip-enhanced Raman spectroscopy. With its ability to image individual atoms, the AFM is a remarkably sensitive detector that can be used to detect separated components. These diverse functional components of microfluidic manipulation have been combined in this work to demonstrate AFM mediated chromatography. AFM mediated chromatography uses channel-less, shear-driven pumping. This is demonstrated with a thin, aluminum oxide substrate and a non-polar solvent system to separate a mixture of lipophilic dyes. In conventional chromatographic terms, this is analogous to thin-layer chromatography using normal phase alumina substrate with sheardriven pumping provided by the AFM tip-cantilever mechanism. The AFM detection of separated components is accomplished by exploiting the variation in the localized friction of the separated components. The AFM tip-cantilever provides the mechanism for producing shear-induced flows and rapid pumping. Shear-driven chromatography (SDC) is a relatively new concept that overcomes the speed and miniaturization limitations of conventional liquid chromatography. SDC is based on a sliding plate system, consisting of two flat surfaces, one of which has a recessed channel. A fluid flow is produced by axially sliding one plate past another, where the fluid has mechanical shear forces imposed at each point along the channel length. The shear-induced flow rates are very reproducible, and do not have pressure or voltage gradient limitations. SDC opens up a new range of enhanced separation kinetics by permitting the sample confinement with submicron dimensions. Small, highly confined liquid is advantageous for chromatographic separation because the separation rate is known to scale according to the square of the confined sample diameter. In addition, because shear-driven flows are not limited by fluid velocity, shear-driven liquid chromatography may provide up to 100,000 plate efficiency.

Measurements of Shear Lift Force on a Bubble in Channel Flow in Microgravity

NASA Technical Reports Server (NTRS)

Nahra, Henry K.; Motil, Brian J.; Skor, Mark

2003-01-01

Under microgravity conditions, the shear lift force acting on bubbles, droplets or solid particles in multiphase flows becomes important because under normal gravity, this hydrodynamic force is masked by buoyancy. This force plays an important role in furnishing the detachment process of bubbles in a setting where a bubble suspension is needed in microgravity. In this work, measurements of the shear lift force acting on a bubble in channel flow are performed. The shear lift force is deduced from the bubble kinematics using scaling and then compared with predictions from models in literature that address different asymptotic and numerical solutions. Basic trajectory calculations are then performed and the results are compared with experimental data of position of the bubble in the channel. A direct comparison of the lateral velocity of the bubbles is also made with the lateral velocity prediction from investigators, whose work addressed the shear lift on a sphere in different two-dimensional shear flows including Poiseuille flow.

Longitudinal shear wave imaging for elasticity mapping using optical coherence elastography

NASA Astrophysics Data System (ADS)

Zhu, Jiang; Miao, Yusi; Qi, Li; Qu, Yueqiao; He, Youmin; Yang, Qiang; Chen, Zhongping

2017-05-01

Shear wave measurements for the determination of tissue elastic properties have been used in clinical diagnosis and soft tissue assessment. A shear wave propagates as a transverse wave where vibration is perpendicular to the wave propagation direction. Previous transverse shear wave measurements could detect the shear modulus in the lateral region of the force; however, they could not provide the elastic information in the axial region of the force. In this study, we report the imaging and quantification of longitudinal shear wave propagation using optical coherence tomography to measure the elastic properties along the force direction. The experimental validation and finite element simulations show that the longitudinal shear wave propagates along the vibration direction as a plane wave in the near field of a planar source. The wave velocity measurement can quantify the shear moduli in a homogeneous phantom and a side-by-side phantom. Combining the transverse shear wave and longitudinal shear wave measurements, this system has great potential to detect the directionally dependent elastic properties in tissues without a change in the force direction.

Measurement of Shear Elastic Moduli in Quasi-Incompressible Soft Solids

NASA Astrophysics Data System (ADS)

Rénier, Mathieu; Gennisson, Jean-Luc; Barrière, Christophe; Catheline, Stefan; Tanter, Mickaël; Royer, Daniel; Fink, Mathias

2008-06-01

Recently a nonlinear equation describing the plane shear wave propagation in isotropic quasi-incompressible media has been developed using a new expression of the strain energy density, as a function of the second, third and fourth order shear elastic constants (respectively μ, A, D) [1]. In such a case, the shear nonlinearity parameter βs depends only from these last coefficients. To date, no measurement of the parameter D have been carried out in soft solids. Using a set of two experiments, acoustoelasticity and finite amplitude shear waves, the shear elastic moduli up to the fourth order of soft solids are measured. Firstly, this theoretical background is applied to the acoustoelasticity theory, giving the variations of the shear wave speed as a function of the stress applied to the medium. From such variations, both linear (μ) and third order shear modulus (A) are deduced in agar-gelatin phantoms. Experimentally the radiation force induced by a focused ultrasound beam is used to generate quasi-plane linear shear waves within the medium. Then the shear wave propagation is imaged with an ultrafast ultrasound scanner. Secondly, in order to give rise to finite amplitude plane shear waves, the radiation force generation technique is replaced by a vibrating plate applied at the surface of the phantoms. The propagation is also imaged using the same ultrafast scanner. From the assessment of the third harmonic amplitude, the nonlinearity parameter βS is deduced. Finally, combining these results with the acoustoelasticity experiment, the fourth order modulus (D) is deduced. This set of experiments provides the characterization, up to the fourth order, of the nonlinear shear elastic moduli in quasi-incompressible soft media. Measurements of the A moduli reveal that while the behaviors of both soft solids are close from a linear point of view, the corresponding nonlinear moduli A are quite different. In a 5% agar-gelatin phantom, the fourth order elastic constant D is found to be 30±10 kPa.

Time and force dependence of the rupture of glycoprotein IIb-IIIa-fibrinogen bonds between latex spheres.

PubMed Central

Goldsmith, H L; McIntosh, F A; Shahin, J; Frojmovic, M M

2000-01-01

We studied the shear-induced breakup of doublets of aldehyde/sulfate (A/S) latex spheres covalently linked with purified platelet GPIIb-IIIa receptor, and cross-linked by fibrinogen. Flow cytometry with fluorescein isothiocyanate-fibrinogen showed than an average of 22,500 molecules of active GPIIb-IIIa were captured per sphere, with a mean K(d) = 56 nM for fibrinogen binding. The spheres, suspended in buffered 19% Ficoll 400 containing 120 or 240 pM fibrinogen, were subjected to Couette flow in a counter-rotating cone-plate rheoscope. Doublets, formed by two-body collisions at low shear rate (G = 8 s(-1)) for < or =15 min, were subjected to shear stress from 0.6 to 2.9 Nm(-2), their rotations recorded until they broke up or were lost to view. Although breakup was time dependent, occurring mostly in the first 2 rotations after the onset of shear, the percentage of doublets broken up after 10 rotations were almost independent of normal hydrodynamic force, F(n): at 240 pN, 15.6, 16.0, and 17.0% broke up in the force range 70-150 pN, 150-230 pN, and 230-310 pN. Unexpectedly, at both [fibrinogen], the initial rate of breakup was highest in the lowest force range, and computer simulation using a stochastic model of breakup was unable to simulate the time course of breakup. When pre-sheared at low G for >15 min, no doublets broke up within 10 rotations at 70 < F(n) < 310 pN; it required >3 min shear (>1110 rotations) at F(n) = 210 pN for significant breakup to occur. Other published work has shown that binding of fibrinogen to GPIIb-IIIa immobilized on plane surfaces exhibits an initial fast reversible process with relative low affinity succeeded by transformation of GPIIb-IIIa to a stable high-affinity complex. We postulate that most doublet breakups observed within 10 rotations were from a population of young doublets having low numbers of bonds, by dissociation of the initial receptor complex relatively unresponsive to force. The remaining, older doublets with GPIIb-IIIa in the high-affinity complex were not broken up in the time or range of forces studied. PMID:10692309

Role of mixed boundaries on flow in open capillary channels with curved air-water interfaces.

PubMed

Zheng, Wenjuan; Wang, Lian-Ping; Or, Dani; Lazouskaya, Volha; Jin, Yan

2012-09-04

Flow in unsaturated porous media or in engineered microfluidic systems is dominated by capillary and viscous forces. Consequently, flow regimes may differ markedly from conventional flows, reflecting strong interfacial influences on small bodies of flowing liquids. In this work, we visualized liquid transport patterns in open capillary channels with a range of opening sizes from 0.6 to 5.0 mm using laser scanning confocal microscopy combined with fluorescent latex particles (1.0 μm) as tracers at a mean velocity of ∼0.50 mm s(-1). The observed velocity profiles indicate limited mobility at the air-water interface. The application of the Stokes equation with mixed boundary conditions (i.e., no slip on the channel walls and partial slip or shear stress at the air-water interface) clearly illustrates the increasing importance of interfacial shear stress with decreasing channel size. Interfacial shear stress emerges from the velocity gradient from the adjoining no-slip walls to the center where flow is trapped in a region in which capillary forces dominate. In addition, the increased contribution of capillary forces (relative to viscous forces) to flow on the microscale leads to increased interfacial curvature, which, together with interfacial shear stress, affects the velocity distribution and flow pattern (e.g., reverse flow in the contact line region). We found that partial slip, rather than the commonly used stress-free condition, provided a more accurate description of the boundary condition at the confined air-water interface, reflecting the key role that surface/interface effects play in controlling flow behavior on the nanoscale and microscale.

Coronal Jet Collimation by Nonlinear Induced Flows

NASA Astrophysics Data System (ADS)

Vasheghani Farahani, S.; Hejazi, S. M.

2017-08-01

Our objective is to study the collimation of solar jets by nonlinear forces corresponding to torsional Alfvén waves together with external forces. We consider a straight, initially non-rotating, untwisted magnetic cylinder embedded in a plasma with a straight magnetic field, where a shear between the internal and external flows exists. By implementing magnetohydrodynamic theory and taking into account the second-order thin flux tube approximation, the balance between the internal nonlinear forces is visualized. The nonlinear differential equation containing the ponderomotive, magnetic tension, and centrifugal forces in the presence of the shear flow is obtained. The solution presents the scale of influence of the propagating torsional Alfvén wave on compressive perturbations. Explicit expressions for the compressive perturbations caused by the forces connected to the torsional Alfvén wave show that, in the presence of a shear flow, the magnetic tension and centrifugal forces do not cancel each other’s effects as they did in its absence. This shear flow plays in favor of the magnetic tension force, resulting in a more efficient collimation. Regarding the ponderomotive force, the shear flow has no effect. The phase relations highlight the interplay of the shear flow and the plasma-β. As the shear flow and plasma-β increase, compressive perturbation amplitudes emerge. We conclude that the jet collimation due to the torsional Alfvén wave highly depends on the location of the jet. The shear flow tightens the collimation as the jet elevates up to the solar corona.

Educational Brief: Using Space for a Better Foundation on Earth Mechanics of Granular Materials

NASA Technical Reports Server (NTRS)

Dooling, Dave (Editor)

2002-01-01

Soils are three-phase composite materials that consist of soil, solid particles, and voids filled with water and/or air. Based on the particle-size distribution, they are generally classified as fine-grained (clays and plastic silts) and coarse-grained soils (nonplastic silts, sand, and gravel). Soil's resistance to external loadings is mainly derived from friction between particles and cohesion. Friction resistance is due to particles' surface-to-surface friction, interlocking, crushing, rearrangement, and dilation (or expansion) during shearing. Cohesion can be due to chemical cementation between particles, electrostatic and electromagnetic forces, and soil-water reaction and equilibrium. The basic factor responsible for the strength of coarse-grained soils is friction. Cohesion can be ignored. This educational brief focuses on measuring shear strength of sands (typical example of coarse-grained soils) where, for the same material, packing density is a main factor to be considered when one asks about the shear strength value. As the external load is applied, the soil's resistance is attained through shearing resistance, which causes the soil volume to increase (expand) or decrease (compress) depending on the initial packing density.

Mechanosensing of shear by Pseudomonas aeruginosa leads to increased levels of the cyclic-di-GMP signal initiating biofilm development

PubMed Central

Rodesney, Christopher A.; Roman, Brian; Dhamani, Numa; Cooley, Benjamin J.; Katira, Parag; Touhami, Ahmed; Gordon, Vernita D.

2017-01-01

Biofilms are communities of sessile microbes that are phenotypically distinct from their genetically identical, free-swimming counterparts. Biofilms initiate when bacteria attach to a solid surface. Attachment triggers intracellular signaling to change gene expression from the planktonic to the biofilm phenotype. For Pseudomonas aeruginosa, it has long been known that intracellular levels of the signal cyclic-di-GMP increase upon surface adhesion and that this is required to begin biofilm development. However, what cue is sensed to notify bacteria that they are attached to the surface has not been known. Here, we show that mechanical shear acts as a cue for surface adhesion and activates cyclic-di-GMP signaling. The magnitude of the shear force, and thereby the corresponding activation of cyclic-di-GMP signaling, can be adjusted both by varying the strength of the adhesion that binds bacteria to the surface and by varying the rate of fluid flow over surface-bound bacteria. We show that the envelope protein PilY1 and functional type IV pili are required mechanosensory elements. An analytic model that accounts for the feedback between mechanosensors, cyclic-di-GMP signaling, and production of adhesive polysaccharides describes our data well. PMID:28533383

Porcelain surface conditioning protocols and shear bond strength of orthodontic brackets.

PubMed

Lestrade, Ashley M; Ballard, Richard W; Xu, Xiaoming; Yu, Qingzhao; Kee, Edwin L; Armbruster, Paul C

2016-05-01

The objective of the present study was to determine which of six bonding protocols yielded a clinically acceptable shear bond strength (SBS) of metal orthodontic brackets to CAD/CAM lithium disilicate porcelain restorations. A secondary aim was to determine which bonding protocol produced the least surface damage at debond. Sixty lithium disilicate samples were fabricated to replicate the facial surface of a mandibular first molar using a CEREC CAD/CAM machine. The samples were split into six test groups, each of which received different mechanical/chemical pretreatment protocols to roughen the porcelain surface prior to bonding a molar orthodontic attachment. Shear bond strength testing was conducted using an Instron machine. The mean, maximum, minimal, and standard deviation SBS values for each sample group including an enamel control were calculated. A t-test was used to evaluate the statistical significance between the groups. No significant differences were found in SBS values, with the exception of surface roughening with a green stone prior to HFA and silane treatment. This protocol yielded slightly higher bond strength which was statistically significant. Chemical treatment alone with HFA/silane yielded SBS values within an acceptable clinical range to withstand forces applied by orthodontic treatment and potentially eliminates the need to mechanically roughen the ceramic surface.

A mathematical model of force transmission from intrafascicularly terminating muscle fibers.

PubMed

Sharafi, Bahar; Blemker, Silvia S

2011-07-28

Many long skeletal muscles are comprised of fibers that terminate intrafascicularly. Force from terminating fibers can be transmitted through shear within the endomysium that surrounds fibers or through tension within the endomysium that extends from fibers to the tendon; however, it is unclear which pathway dominates in force transmission from terminating fibers. The purpose of this work was to develop mathematical models to (i) compare the efficacy of lateral (through shear) and longitudinal (through tension) force transmission in intrafascicularly terminating fibers, and (ii) determine how force transmission is affected by variations in the structure and properties of fibers and the endomysium. The models demonstrated that even though the amount of force that can be transmitted from an intrafascicularly terminating fiber is dependent on fiber resting length (the unstretched length at which passive stress is zero), endomysium shear modulus, and fiber volume fraction (the fraction of the muscle cross-sectional area that is occupied by fibers), fibers that have values of resting length, shear modulus, and volume fraction within physiologic ranges can transmit nearly all of their peak isometric force laterally through shearing of the endomysium. By contrast, the models predicted only limited force transmission ability through tension within the endomysium that extends from the fiber to the tendon. Moreover, when fiber volume fraction decreases to unhealthy ranges (less than 50%), the force-transmitting potential of terminating fibers through shearing of the endomysium decreases significantly. The models presented here support the hypothesis that lateral force transmission through shearing of the endomysium is an effective mode of force transmission in terminating fibers. Copyright © 2011 Elsevier Ltd. All rights reserved.

Measurement of Giardia lamblia adhesion force using an integrated microfluidic assay.

PubMed

Lu, Ling; Zheng, Guo-Xia; Yang, Yu-Suo; Feng, Cheng-Yu; Liu, Fang-Fang; Wang, Yun-Hua

2017-02-01

The mechanisms how Giardias attach to the intestinal epithelium remain unclear. None of the methods currently being used to measure the attachment force could provide a continuous nutrition supply and a micro-aerobic atmosphere to the Giardia. Besides, they are all labor-intensive. In the present research, a microfluidic method based on electric circuit analogy was developed. The input fluid flowed through the inlet channel with different lengths and was distributed in four assay chambers. Shear force gradients were generated in chambers, too. This allowed an easy control of fluids and the shear forces. Most importantly, the shear stress large enough to detach Giardia could be generated in laminar flow regime. Moreover, analysis could be accomplished in one single test. By applying inlet flow rates of 30, 60, and 120 μL ml -1 , shear force gradients ranging from 19.47 to 60.50 Pa were generated. The adhesion forces of trophozoites were analyzed and the EC 50 of the force that caused 50% trophozoites detachment was calculated as 36.60 Pa. This paper presents a novel method for measurement of Giardia adhesion force. Graphical Abstract Measurement of Giardia adhesion force. Various of flow rates were applied to generate different shear forces and Giardia trophozoites remaining attached were counted (a-c). The percentages of attachment vs shear stress were plotted and the EC 50 of adhesion force was calculated (d).

Wind shear and wet and dry thermodynamic indices as predictors of thunderstorm motion and severity and application to the AVE 4 experimental data

NASA Technical Reports Server (NTRS)

Connell, J. R.; Ey, L.

1977-01-01

Two types of parameters are computed and mapped for use in assessing their individual merits as predictors of occurrence and severity of thunderstorms. The first group is comprised of equivalent potential temperature, potential temperature, water vapor mixing ratio, and wind speed. Equivalent potential temperature maxima and strong gradients of equivalent potential temperature at the surface correlate well with regions of thunderstorm activity. The second type, comprised of the energy index, shear index, and energy shear index, incorporates some model dynamics of thunderstorms, including nonthermodynamic forcing. The energy shear index is found to improve prediction of tornadic and high-wind situations slightly better than other indices. It is concluded that further development and refinement of nonthermodynamic aspects of predictive indices are definitely warranted.

Squeezing and de-wetting of a shear thinning fluid drop between plane parallel surfaces: capillary adhesion phenomenon

NASA Astrophysics Data System (ADS)

Ward, Thomas

2017-11-01

The radial squeezing and de-wetting of a thin film of viscous shear thinning fluid filling the gap between parallel plane walls is examined both experimentally and theoretically for gap spacing much smaller than the capillary length. The interaction between motion of fluid in the gap driven by squeezing or de-wetting and surface tension is parameterized by a dimensionless variable, F, that is the ratio of the constant force supplied by the top plate (either positive or negative) to surface tension at the drop's circumference. Furthermore, the dimensionless form of the rate equation for the gap's motion reveals a time scale that is dependent on the drop volume when analyzed for a power law shear thinning fluid. In the de-wetting problem the analytical solution reveals the formation of a singularity, leading to capillary adhesion, as the gap spacing approaches a critical value that depends on F and the contact angle. Experiments are performed to test the analytical predictions for both squeezing, and de-wetting in the vicinity of the singularity.

High-Aspect-Ratio Rotating Cell-Culture Vessel

NASA Technical Reports Server (NTRS)

Wolf, David A.; Sams, Clarence; Schwarz, Ray P.

1992-01-01

Cylindrical rotating cell-culture vessel with thin culture-medium layer of large surface area provides exchange of nutrients and products of metabolism with minimal agitation. Rotation causes averaging of buoyant forces otherwise separating components of different densities. Vessel enables growth of cells in homogeneous distribution with little agitation and little shear stress.

Design parameters for rotating cylindrical filtration

NASA Technical Reports Server (NTRS)

Schwille, John A.; Mitra, Deepanjan; Lueptow, Richard M.

2002-01-01

Rotating cylindrical filtration displays significantly reduced plugging of filter pores and build-up of a cake layer, but the number and range of parameters that can be adjusted complicates the design of these devices. Twelve individual parameters were investigated experimentally by measuring the build-up of particles on the rotating cylindrical filter after a fixed time of operation. The build-up of particles on the filter depends on the rotational speed, the radial filtrate flow, the particle size and the gap width. Other parameters, such as suspension concentration and total flow rate are less important. Of the four mechanisms present in rotating filters to reduce pore plugging and cake build-up, axial shear, rotational shear, centrifugal sedimentation and vortical motion, the evidence suggests rotational shear is the dominant mechanism, although the other mechanisms still play minor roles. The ratio of the shear force acting parallel to the filter surface on a particle to the Stokes drag acting normal to the filter surface on the particle due to the difference between particle motion and filtrate flow can be used as a non-dimensional parameter that predicts the degree of particle build-up on the filter surface for a wide variety of filtration conditions. c2002 Elsevier Science B.V. All rights reserved.

Continuum-kinetic-microscopic model of lung clearance due to core-annular fluid entrainment

PubMed Central

Mitran, Sorin

2013-01-01

The human lung is protected against aspirated infectious and toxic agents by a thin liquid layer lining the interior of the airways. This airway surface liquid is a bilayer composed of a viscoelastic mucus layer supported by a fluid film known as the periciliary liquid. The viscoelastic behavior of the mucus layer is principally due to long-chain polymers known as mucins. The airway surface liquid is cleared from the lung by ciliary transport, surface tension gradients, and airflow shear forces. This work presents a multiscale model of the effect of airflow shear forces, as exerted by tidal breathing and cough, upon clearance. The composition of the mucus layer is complex and variable in time. To avoid the restrictions imposed by adopting a viscoelastic flow model of limited validity, a multiscale computational model is introduced in which the continuum-level properties of the airway surface liquid are determined by microscopic simulation of long-chain polymers. A bridge between microscopic and continuum levels is constructed through a kinetic-level probability density function describing polymer chain configurations. The overall multiscale framework is especially suited to biological problems due to the flexibility afforded in specifying microscopic constituents, and examining the effects of various constituents upon overall mucus transport at the continuum scale. PMID:23729842

Liposomes as lubricants: beyond drug delivery.

PubMed

Goldberg, Ronit; Klein, Jacob

2012-05-01

In this paper we review recent work (Goldberg et al., 2011a,b) on a new use for phosphatidylcholine liposomes: as ultra-efficient boundary lubricants at up to the highest physiological pressures. Using a surface force balance, we have measured the normal and shear interactions as a function of surface separation between layers of hydrogenated soy phophatidylcholine (HSPC) small unilamellar vesicles (SUVs) adsorbed from dispersion, at both pure water and physiologically high salt concentrations of 0.15 M NaNO(3). Cryo-Scanning Electron Microscopy shows each surface to be coated by a close-packed HSPC-SUV layer with an over-layer of liposomes on top. The shear forces reveal strikingly low friction coefficients down to 2×10(-5) in pure water system or 6×10(-4) in the 150 mM salt system, up to contact pressures of at least 12 MPa (pure water) or 6 MPa (high salt), comparable with those in the major joints. This low friction is attributed to the hydration lubrication mechanism arising from rubbing of the highly hydrated phosphocholine-headgroup layers exposed at the outer surface of each liposome, and provides support for the conjecture that phospholipids may play a significant role in biological lubrication. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Continuum-kinetic-microscopic model of lung clearance due to core-annular fluid entrainment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mitran, Sorin, E-mail: mitran@unc.edu

2013-07-01

The human lung is protected against aspirated infectious and toxic agents by a thin liquid layer lining the interior of the airways. This airway surface liquid is a bilayer composed of a viscoelastic mucus layer supported by a fluid film known as the periciliary liquid. The viscoelastic behavior of the mucus layer is principally due to long-chain polymers known as mucins. The airway surface liquid is cleared from the lung by ciliary transport, surface tension gradients, and airflow shear forces. This work presents a multiscale model of the effect of airflow shear forces, as exerted by tidal breathing and cough,more » upon clearance. The composition of the mucus layer is complex and variable in time. To avoid the restrictions imposed by adopting a viscoelastic flow model of limited validity, a multiscale computational model is introduced in which the continuum-level properties of the airway surface liquid are determined by microscopic simulation of long-chain polymers. A bridge between microscopic and continuum levels is constructed through a kinetic-level probability density function describing polymer chain configurations. The overall multiscale framework is especially suited to biological problems due to the flexibility afforded in specifying microscopic constituents, and examining the effects of various constituents upon overall mucus transport at the continuum scale.« less

Continuum-kinetic-microscopic model of lung clearance due to core-annular fluid entrainment

NASA Astrophysics Data System (ADS)

Mitran, Sorin

2013-07-01

The human lung is protected against aspirated infectious and toxic agents by a thin liquid layer lining the interior of the airways. This airway surface liquid is a bilayer composed of a viscoelastic mucus layer supported by a fluid film known as the periciliary liquid. The viscoelastic behavior of the mucus layer is principally due to long-chain polymers known as mucins. The airway surface liquid is cleared from the lung by ciliary transport, surface tension gradients, and airflow shear forces. This work presents a multiscale model of the effect of airflow shear forces, as exerted by tidal breathing and cough, upon clearance. The composition of the mucus layer is complex and variable in time. To avoid the restrictions imposed by adopting a viscoelastic flow model of limited validity, a multiscale computational model is introduced in which the continuum-level properties of the airway surface liquid are determined by microscopic simulation of long-chain polymers. A bridge between microscopic and continuum levels is constructed through a kinetic-level probability density function describing polymer chain configurations. The overall multiscale framework is especially suited to biological problems due to the flexibility afforded in specifying microscopic constituents, and examining the effects of various constituents upon overall mucus transport at the continuum scale.

Insect adhesion on rough surfaces: analysis of adhesive contact of smooth and hairy pads on transparent microstructured substrates

PubMed Central

Zhou, Yanmin; Robinson, Adam; Steiner, Ullrich; Federle, Walter

2014-01-01

Insect climbing footpads are able to adhere to rough surfaces, but the details of this capability are still unclear. To overcome experimental limitations of randomly rough, opaque surfaces, we fabricated transparent test substrates containing square arrays of 1.4 µm diameter pillars, with variable height (0.5 and 1.4 µm) and spacing (from 3 to 22 µm). Smooth pads of cockroaches (Nauphoeta cinerea) made partial contact (limited to the tops of the structures) for the two densest arrays of tall pillars, but full contact (touching the substrate in between pillars) for larger spacings. The transition from partial to full contact was accompanied by a sharp increase in shear forces. Tests on hairy pads of dock beetles (Gastrophysa viridula) showed that setae adhered between pillars for larger spacings, but pads were equally unable to make full contact on the densest arrays. The beetles' shear forces similarly decreased for denser arrays, but also for short pillars and with a more gradual transition. These observations can be explained by simple contact models derived for soft uniform materials (smooth pads) or thin flat plates (hairy-pad spatulae). Our results show that microstructured substrates are powerful tools to reveal adaptations of natural adhesives for rough surfaces. PMID:24990289

Insect adhesion on rough surfaces: analysis of adhesive contact of smooth and hairy pads on transparent microstructured substrates.

PubMed

Zhou, Yanmin; Robinson, Adam; Steiner, Ullrich; Federle, Walter

2014-09-06

Insect climbing footpads are able to adhere to rough surfaces, but the details of this capability are still unclear. To overcome experimental limitations of randomly rough, opaque surfaces, we fabricated transparent test substrates containing square arrays of 1.4 µm diameter pillars, with variable height (0.5 and 1.4 µm) and spacing (from 3 to 22 µm). Smooth pads of cockroaches (Nauphoeta cinerea) made partial contact (limited to the tops of the structures) for the two densest arrays of tall pillars, but full contact (touching the substrate in between pillars) for larger spacings. The transition from partial to full contact was accompanied by a sharp increase in shear forces. Tests on hairy pads of dock beetles (Gastrophysa viridula) showed that setae adhered between pillars for larger spacings, but pads were equally unable to make full contact on the densest arrays. The beetles' shear forces similarly decreased for denser arrays, but also for short pillars and with a more gradual transition. These observations can be explained by simple contact models derived for soft uniform materials (smooth pads) or thin flat plates (hairy-pad spatulae). Our results show that microstructured substrates are powerful tools to reveal adaptations of natural adhesives for rough surfaces.

Interaction of thermal and mechanical processes in steep permafrost rock walls: A conceptual approach

NASA Astrophysics Data System (ADS)

Draebing, D.; Krautblatter, M.; Dikau, R.

2014-12-01

Degradation of permafrost rock wall decreases stability and can initiate rock slope instability of all magnitudes. Rock instability is controlled by the balance of shear forces and shear resistances. The sensitivity of slope stability to warming results from a complex interplay of shear forces and resistances. Conductive, convective and advective heat transport processes act to warm, degrade and thaw permafrost in rock walls. On a seasonal scale, snow cover changes are a poorly understood key control of the timing and extent of thawing and permafrost degradation. We identified two potential critical time windows where shear forces might exceed shear resistances of the rock. In early summer combined hydrostatic and cryostatic pressure can cause a peak in shear force exceeding high frozen shear resistance and in autumn fast increasing shear forces can exceed slower increasing shear resistance. On a multiannual system scale, shear resistances change from predominantly rock-mechanically to ice-mechanically controlled. Progressive rock bridge failure results in an increase of sensitivity to warming. Climate change alters snow cover and duration and, hereby, thermal and mechanical processes in the rock wall. Amplified thawing of permafrost will result in higher rock slope instability and rock fall activity. We present a holistic conceptual approach connecting thermal and mechanical processes, validate parts of the model with geophysical and kinematic data and develop future scenarios to enhance understanding on system scale.

Coronal Jet Collimation by Nonlinear Induced Flows

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vasheghani Farahani, S.; Hejazi, S. M.

2017-08-01

Our objective is to study the collimation of solar jets by nonlinear forces corresponding to torsional Alfvén waves together with external forces. We consider a straight, initially non-rotating, untwisted magnetic cylinder embedded in a plasma with a straight magnetic field, where a shear between the internal and external flows exists. By implementing magnetohydrodynamic theory and taking into account the second-order thin flux tube approximation, the balance between the internal nonlinear forces is visualized. The nonlinear differential equation containing the ponderomotive, magnetic tension, and centrifugal forces in the presence of the shear flow is obtained. The solution presents the scale ofmore » influence of the propagating torsional Alfvén wave on compressive perturbations. Explicit expressions for the compressive perturbations caused by the forces connected to the torsional Alfvén wave show that, in the presence of a shear flow, the magnetic tension and centrifugal forces do not cancel each other’s effects as they did in its absence. This shear flow plays in favor of the magnetic tension force, resulting in a more efficient collimation. Regarding the ponderomotive force, the shear flow has no effect. The phase relations highlight the interplay of the shear flow and the plasma- β . As the shear flow and plasma- β increase, compressive perturbation amplitudes emerge. We conclude that the jet collimation due to the torsional Alfvén wave highly depends on the location of the jet. The shear flow tightens the collimation as the jet elevates up to the solar corona.« less

Insights into adhesion of abalone: A mechanical approach.

PubMed

Li, Jing; Zhang, Yun; Liu, Sai; Liu, Jianlin

2018-01-01

Many living creatures possess extremely strong capability of adhesion, which has aroused great attention of many scientists and engineers. Based on the self-developed equipment, we measured the normal and shear adhesion strength of the abalone underwater and out of water on different contact surfaces. It is found that the adhesion force of the abalone can amount to 200 or 300 times its body weight. The effects of wettability and roughness of the surface, and the frictional coefficient of mucus on the adhesion strength have been discussed. The theoretical calculation manifests that the normal adhesion force mainly stems from the suction pressure, van der Waals force and capillary force of the pedal, and their limit values are given. These findings may provide some inspirations to engineer new-typed materials, micro-devices, adhesives and medicine. Copyright © 2017 Elsevier Ltd. All rights reserved.

Comparison of Warner-Bratzler shear force values between round and square cross-section cores from cooked beef and pork Longissimus muscle.

PubMed

Silva, Douglas R G; Torres Filho, Robledo A; Cazedey, Henrique P; Fontes, Paulo R; Ramos, Alcinéia L S; Ramos, Eduardo M

2015-05-01

This study was conducted to investigate the effect of core sampling on Warner-Bratzler shear force evaluations of beef and pork loins (Longissimus thoracis et lumborum muscles) and to determine the relationship between them. Steaks of 2.54 cm from beef and pork loins were cooked and five round cross-section cores and five square cross-section cores of each steak were taken for shear force evaluation. Core sampling influenced both beef and pork shear force values with higher (P<0.05) average values and standard deviations for square cross-section cores. There was a strong and linear relationship (P<0.01) between round and square cross-section cores for beef (R(2)=0.78), pork (R(2)=0.70) and for beef+pork (R(2)=0.82) samples. These results indicate that it is feasible to use square cross-section cores in Warner-Bratzler shear force protocol as an alternative and potential method to standardize sampling for shear force measurements. Copyright © 2014 Elsevier Ltd. All rights reserved.

In vitro Assessment of Influence of Various Bleaching Protocols on the Strength of Ceramic Orthodontic Brackets bonded to Bleached Tooth Surface: A Comparative Study.

PubMed

Iska, Divya; Devanna, Raghu; Singh, Madhvi; Chitumalla, Rajkiran; Balasubramanian, Sai C Bala; Goutam, Manish

2017-12-01

Esthetics is one of the common issues because of which patients consult dental orthodontic treatment. Two ways of tooth bleaching are available these days, which includes in-office bleach and home bleach. Various bleaching protocols are available these days for treating the tooth surfaces. Hence, we planned the present study for investigating the impact of various intracoronal bleaching protocols on shear bond strength of ceramic brackets bonded to tooth surface after bleaching. The present study included assessment of 100 extracted maxillary central incisors with the integrated buccal surface. A resin block was made and individual teeth were embedded in each block. Root canal therapy procedure was performed in all the teeth, after which 2 mm short of tooth apex up to the level of cementoenamel junction, removal of the root canal filling was done. All the samples were broadly divided into four study groups with 25 samples in each group. Bleaching procedure was carried in all the samples intracoronally followed by testing of shear bond strength using universal force testing machine. Following the modified adhesive remnant index (AI), assessment of remaining adhesive on the brackets was done. All the results were compiled and analyzed by Statistical Package for the Social Sciences (SPSS) software version 17.0. In the control group, mean shear bond strength was found to be 17.9 MPa. While comparing the carbamide peroxide (CP) group with sodium perborate study group, we observed a statistically significant difference. Nonsignificant results were obtained while comparing the shear bond strength in between sodium perborate group and hydrogen peroxide (HP) group. Intracoronal bleaching does affect the shear bond strength of ceramic brackets. Sodium perborate bleaching influences shear bond strength more strongly than other bleaching agents such as CP and HP. In patients undergoing orthodontic treatment, HP is a preferred agent where bleaching has to be followed by orthodontic bonding to the tooth surface.

Effects of different shear rates on the attachment and detachment of platelet thrombi.

PubMed

Shi, Xiaofeng; Yang, Jichun; Huang, Jiansong; Long, Zhangbiao; Ruan, Zheng; Xiao, Bing; Xi, Xiaodong

2016-03-01

Thrombosis and hemostasis take place in flowing blood, which generates shear forces. The effect of different shear rates, particularly pathological forces, on platelet thrombus formation remains to be fully elucidated. The present study observed the morphological characteristics and hierarchical structure of thrombi on the collagen surface at a wide range of wall shear rates (WSRs) and examined the underlying mechanisms. Calcein AM‑labeled whole blood was perfused over a collagen‑coated surface at different shear rates set by a Bioflux 200 microfluidic device and the thrombi formed were assessed for area coverage, the height and the hierarchical structure defined by the extent of platelet activation and packing density. The factors that affect thrombus formation were also investigated. Platelet thrombus formation varied under different WSRs, for example, dispersed platelet adhesion mixed with erythrocytes was observed at 125‑250 s(‑1), extensive and thin platelet thrombi were observed at 500‑1,500 s(‑1), and sporadic, thick thrombi were observed at pathological WSRs of 2,500‑5,000 s(‑1), which showed a tendency to be shed. With increasing WSRs, the height of the thrombi showed an increasing linear trend, whereas the total fluorescence intensity and area of the thrombi exhibited a parabolic curve‑like change, with a turning point at a WSR of 2,500 s(‑1). The number of thrombi, the average fluorescence intensity and the area per thrombus showed similar trends, with an initial upwards incline followed by a decline. The thrombi formed at higher WSRs had a thicker shell, which led to a more densely packed core. Platelet thrombus formation under shear‑flow was regulated by the adhesive strength, which was mediated by receptor‑ligand interaction, the platelet deposition induced by shear rates and the detachment by the dynamic force of flow. This resulted in a balance between thrombus attachment, including adhesion and aggregation, and detachment. Collectively, compared with physiological low WSRs, pathological high WSRs caused thicker and more easily shed thrombi with more condensed cores, which was regulated by an attachment‑detachment balance. These results provide novel insights into the properties of thrombus formation on collagen at different WSRs, and offers possible explanations for certain clinical physiopathological phenomena, including physical hemostasis and pathological thrombosis.

Effect of load, cadence, and fatigue on tibio-femoral joint force during a half squat.

PubMed

Hattin, H C; Pierrynowski, M R; Ball, K A

1989-10-01

Ten male university student volunteers were selected to investigate the 3D articular force at the tibio-femoral joint during a half squat exercise, as affected by cadence, different barbell loads, and fatigue. Each subject was required to perform a half squat exercise with a barbell weight centered across the shoulders at two different cadences (1 and 2 s intervals) and three different loads (15, 22 and 30% of the one repetition maximum). Fifty repetitions at each experimental condition were recorded with an active optoelectronic kinematic data capture system (WATSMART) and a force plate (Kistler). Processing the data involved a photogrammetric technique to obtain subject tailored anthropometric data. The findings of this study were: 1) the maximal antero-posterior shear and compressive force consistently occurred at the lowest position of the weight, and the forces were very symmetrically disposed on either side of this halfway point; 2) the medio-lateral shear forces were small over the squat cycle with few peaks and troughs; 3) cadence increased the antero-posterior shear (50%) and the compressive forces (28%); 4) as a subject fatigues, load had a significant effect on the antero-posterior shear force; 5) fatigue increased all articular force components but it did not manifest itself until about halfway through the 50 repetitions of the exercise; 6) the antero-posterior shear force was most affected by fatigue; 7) cadence had a significant effect on fatigue for the medio-lateral shear and compressive forces.

Beef longissimus slice shear force measurement among steak locations and institutions.

PubMed

Wheeler, T L; Shackelford, S D; Koohmaraie, M

2007-09-01

The objectives of this study were 1) to determine which longissimus thoracis et lumborum steaks were appropriate for slice shear force measurement and 2) to determine the among and within institution variation in LM slice shear force values of 6 institutions after they received expert training on the procedure and a standard kit of equipment. In experiment 1, longissimus thoracis et lumborum muscles were obtained from the left sides of 50 US Select carcasses. Thirteen longissimus thoracis and 12 longissimus lumborum steaks were cut 2.54 cm thick from each muscle. Slice shear force was measured on each steak. Mean slice shear force among steak locations (1 to 25) ranged from 19.7 to 27.3 kg. Repeatability of slice shear force (based on variance) among steak locations ranged from 0.71 to 0.96. In experiment 2, the longissimus thoracis et lumborum were obtained from the left sides of 154 US Select beef carcasses. Eight 2.54-cm-thick steaks were obtained from the caudal end of each frozen longissimus thoracis, and six 2.54-cm-thick steaks were obtained from the cranial end of each frozen longissimus lumborum. Seven pairs of consecutive steaks were assigned for measurement of slice shear force. Seven institutions were assigned to steak pairs within each carcass using a randomized complete block design, such that each institution was assigned to each steak pair 22 times. Repeatability estimates for slice shear force for the 7 institutions were 0.89, 0.83, 0.91, 0.90, 0.89, 0.76, and 0.89, respectively, for institutions 1 to 7. Mean slice shear force values were least (P <0.05) for institutions 3 (22.7 kg) and 7 (22.3 kg) and were greatest (P <0.05) for institutions 5 (27.3 kg) and 6 (27.6 kg). Institutions with greater mean slice shear force (institutions 5 and 6) used cooking methods that required more (P <0.05) time (32.0 and 36.9 min vs. 5.5 to 11.8 min) to reach the end point temperature (71 degrees C) and resulted in greater (P <0.05) cooking loss (both 26.6% vs. 14.4 to 24.1%). Differences among institutions in the repeatability of slice shear force were partially attributable to differences among institutions in the consistency of steak thawing and cooking procedures. These results emphasize the importance of sample location within the muscle and cooking method in the measurement of tenderness and indicate that with proper training and application of the protocol, slice shear force is a highly repeatable (R approximately 0.90) measure of beef LM tenderness.

Simulations of shear-thinning frictional non-Brownian suspensions

NASA Astrophysics Data System (ADS)

Lemaire, Elisabeth; Lobry, Laurent; Blanc, Frederic; Peters, Francois; RSC Team

2017-11-01

Most non-Brownian suspensions exhibit non-Newtonian behaviours such as anisotropic normal stresses, shear-thickening or shear-thinning. The later is still largely an open question. Acrivos wet al. (JoR 1994) proposed that particle resuspension is responsible for the apparent shear-thinning behavior in a cylindrical Couette rheometer. Another explanation has been suggested by Vasquez-Quesada et al. (PRL 2017), who noticed that some polymeric suspending liquids themselves are shear-thinning for the high shear-rate values involved in the narrow gaps between particles. Here we propose that the shear-thinning behaviour is directly connected to the solid contact between particles that has been shown to play a crucial role in the rheological behaviour of concentrated non-Brownian suspensions. In particular, it has been recently shown that frictional contact between particles greatly enhances the viscosity. Even though the friction coefficient between macroscopic surfaces does not depend on the load, it may be not the case at the scale of the low load contact between particles in suspensions. Here, we present discrete numerical simulations where the friction coefficient decreases with the interparticle forces. The obtained shear-thinning behaviour is in good agreement with our experiments.

The Influence of Subglacial Hydrology on Ice Stream Velocity in a Physical Model

NASA Astrophysics Data System (ADS)

Wagman, B. M.; Catania, G.; Buttles, J. L.

2011-12-01

We use a physical model to investigate how changes in subglacial hydrology affect ice motion in ice streams found in the West Antarctic Ice Sheet. Ice streams are modeled using silicone polymer placed over a thin water layer to simulate ice flow dominated by basal sliding. Dynamic similarity between modeled and natural ice streams is achieved through direct comparison of the glacier force balance using the conditions on Whillans Ice Stream (WIS) as our goal.This ice stream has a force balance that has evolved through time due to increased basal resistance. Currently, between 50-90% of the driving stress is supported by the ice stream shear margins [Stearns et al., JGlac 2005]. A similar force balance can be achieved in our model with a surface slope of 0.025. We test two hypotheses; 1) the distribution and thickness of the subglacial water layer influences the ice flow speed and thus the force balance and can reproduce the observed slowdown of WIS and; 2) shear margins are locations where transitions in water layer thickness occur.

Lateral shearing optical gradient force in coupled nanobeam photonic crystal cavities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Du, Han; Zhang, Xingwang; Chau, Fook Siong

2016-04-25

We report the experimental observation of lateral shearing optical gradient forces in nanoelectromechanical systems (NEMS) controlled dual-coupled photonic crystal (PhC) nanobeam cavities. With an on-chip integrated NEMS actuator, the coupled cavities can be mechanically reconfigured in the lateral direction while maintaining a constant coupling gap. Shearing optical gradient forces are generated when the two cavity centers are laterally displaced. In our experiments, positive and negative lateral shearing optical forces of 0.42 nN and 0.29 nN are observed with different pumping modes. This study may broaden the potential applications of the optical gradient force in nanophotonic devices and benefit the futuremore » nanooptoelectromechanical systems.« less

Complex strain fields

NASA Astrophysics Data System (ADS)

Bradshaw, P.

Computational techniques for accounting for extra strain rates, abnormal distributions of delta-U/delta-y, fluctuating strain rates, and the effects of body forces in modeling shear flows are discussed. Consideration is given to simple shears where the extra strain rate does not affect turbulence, thin shear layers, moderately thin shear layers, and strongly distorted flows. Attention is given to formulations based on the exact transport equations for Reynolds stress as derived from the time-averaged Navier-Stokes equations. Extra strain rates arise from curvature, lateral divergence, and bulk compression, with Coriolis forces accounting for the first, intensification of the spanwise vorticity for the second, and compression or dilation of the shear layer producing the third. The curvature forces, e.g., buoyancy and Coriolis forces, are responsible for hurricanes and tornadoes.

Factors influencing the shear rate acting on silicone oil to cause silicone oil emulsification.

PubMed

Chan, Yau Kei; Cheung, Ning; Wong, David

2014-10-30

The shear force between silicone oil (SO) bubble and aqueous during eye movements may underlie the development of SO emulsification. This study examines factors that may affect such shear force induced by eye movements. A surface-modified model eye chamber was put under large-amplitude eye movements (amplitude 90°, angular velocity 360°/s, and a duration 300 ms). Agarose-made indentations were introduced to mimic the effect of encircling scleral buckle. Two SOs (1300 and 5000 centistokes [cSt]), three volumes (3, 4, and 5 mL), and two eye chambers (with and without indentation) were tested. Video recording was used to capture the movements of SO inside the model chamber under various conditions. The presence of indentation within the eye chamber significantly reduced the velocity of SO movements relative to the eye chamber movements (P < 0.001). To a lesser extent, an increase in viscosity also had a significant effect in reducing the relative movements. No significant effect was observed for the extent of SO fill in the chamber. Our experimental model suggests indentation within an eye, such as that created by scleral buckling, may have the greatest influence in reducing shear force induced by eye movements. Therefore, using an encircling scleral buckle may be similarly or more effective than using SO with higher viscosity in lowering the propensity to SO emulsification. Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.

Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery

PubMed Central

Catanese, D J; Fogg, J M; Schrock, D E; Gilbert, B E; Zechiedrich, L

2012-01-01

Supercoiled DNAs varying from 281 to 5302 bp were subjected to shear forces generated by aerosolization or sonication. DNA shearing strongly correlated with length. Typical sized plasmids (⩾3000 bp) degraded rapidly. DNAs 2000–3000 bp persisted ∼10 min. Even in the absence of condensing agents, supercoiled DNA <1200 bp survived nebulization, and increased forces of sonication were necessary to shear it. Circular vectors were considerably more resistant to shearing than linear vectors of the same length. DNA supercoiling afforded additional protection. These results show the potential of shear-resistant Minivector DNAs to overcome one of the major challenges associated with gene therapy delivery. PMID:21633394

Drought causes substantial reductions in non-isothermal soil strength

NASA Astrophysics Data System (ADS)

Vahedifard, F.; Robinson, J. D.; Love, C. A.; AghaKouchak, A.

2016-12-01

The stability and settlement of natural slopes and engineering structures are governed primarily by the shear strength of foundation soil. Understanding soil-atmosphere interactions and their impacts on shear strength is imperative to evaluating drought impacts on the resilience of our infrastructure. This understanding is also important for assessing a variety of emerging science and engineering problems in a changing climate including analyzing existing and new infrastructures, landslides, soil carbon sequestration, land management, and managing traction and tillage in agriculture. While progress has been made in understanding shear strength response to soil moisture changes, the impacts of concurrent soil moisture and temperature changes on shear strength remain uncertain from a regional-scale perspective. Here we present a methodological framework based on various soil types, temperatures, and moistures, and surface fluxes, to quantify a non-isothermal soil shear strength. We employ a non-isothermal soil strength analysis (NISSA) to explore the extent to which elevated soil temperatures and low moistures, along with abnormal surface fluxes, during California's record-setting 2012 - 2015 drought reduced the soil's shear strength. Our results suggest that the prolonged California drought reduced the shear strength of fine-grained soil as much as 95%. In contrast, the NISSA suggests that drought impacts on coarse-grained soil were not as significant. These opposing behaviors are attributed to the existence and absence of intermolecular physico-chemical forces in fine- and coarse-grained soils, respectively. The outlined framework offers a unique avenue to explore how soil shear strength is likely to behave under extreme drought conditions.

Anomalously low strength of serpentinite sheared against granite and implications for creep on the Hayward and Calaveras Faults

USGS Publications Warehouse

Moore, Diane E.; Lockner, David A.; Ponce, David A.

2010-01-01

Serpentinized ophiolitic rocks are juxtaposed against quartzofeldspathic rocks at depth across considerable portions of the Hayward and Calaveras Faults. The marked compositional contrast between these rock types may contribute to fault creep that has been observed along these faults. To investigate this possibility, we are conducting hydrothermal shearing experiments to look for changes in frictional properties resulting from the shear of ultramafic rock juxtaposed against quartzose rock units. In this paper we report the first results in this effort: shear of bare-rock surfaces of serpentinite and granite, and shear of antigorite-serpentinite gouge between forcing blocks of granitic rock. All experiments were conducted at 250°C. Serpentinite sheared against granite at 50 MPa pore-fluid pressure is weaker than either rock type separately, and the weakening is significantly more pronounced at lower shearing rates. In contrast, serpentinite gouge sheared dry between granite blocks is as strong as the bare granite surface. We propose that the weakening is the result of a solution-transfer process involving the dissolution of serpentine minerals at grain-to-grain contacts. Dissolution of serpentine is enhanced by modifications to pore-fluid chemistry caused by interaction of the fluid with the quartz-bearing rocks. The compositional differences between serpentinized ultramafic rocks of the Coast Range Ophiolite and quartzofeldspathic rock units such as those of the Franciscan Complex may provide the mechanism for aseismic slip (creep) in the shallow crust along the Hayward, Calaveras, and other creeping faults in central and northern California.

Dynamic Adhesion of Umbilical Cord Blood Endothelial Progenitor Cells under Laminar Shear Stress

PubMed Central

Angelos, Mathew G.; Brown, Melissa A.; Satterwhite, Lisa L.; Levering, Vrad W.; Shaked, Natan T.; Truskey, George A.

2010-01-01

Late outgrowth endothelial progenitor cells (EPCs) represent a promising cell source for rapid reendothelialization of damaged vasculature after expansion ex vivo and injection into the bloodstream. We characterized the dynamic adhesion of umbilical-cord-blood-derived EPCs (CB-EPCs) to surfaces coated with fibronectin. CB-EPC solution density affected the number of adherent cells and larger cells preferentially adhered at lower cell densities. The number of adherent cells varied with shear stress, with the maximum number of adherent cells and the shear stress at maximum adhesion depending upon fluid viscosity. CB-EPCs underwent limited rolling, transiently tethering for short distances before firm arrest. Immediately before arrest, the instantaneous velocity decreased independent of shear stress. A dimensional analysis indicated that adhesion was a function of the net force on the cells, the ratio of cell diffusion to sliding speed, and molecular diffusivity. Adhesion was not limited by the settling rate and was highly specific to α5β1 integrin. Total internal reflection fluorescence microscopy showed that CB-EPCs produced multiple contacts of α5β1 with the surface and the contact area grew during the first 20 min of attachment. These results demonstrate that CB-EPC adhesion from blood can occur under physiological levels of shear stress. PMID:21112278

Surface instability of a thin electrolyte film undergoing coupled electroosmotic and electrophoretic flows in a microfluidic channel.

PubMed

Ray, Bahni; Reddy, Puchalapalli Dinesh Sankar; Bandyopadhyay, Dipankar; Joo, Sang W; Sharma, Ashutosh; Qian, Shizhi; Biswas, Gautam

2011-11-01

We consider the stability of a thin liquid film with a free charged surface resting on a solid charged substrate by performing a general Orr-Sommerfeld (O-S) analysis complemented by a long-wave (LW) analysis. An externally applied field generates an electroosmotic flow (EOF) near the solid substrate and an electrophoretic flow (EPF) at the free surface. The EPF retards the EOF when both the surfaces have the same sign of the potential and can even lead to the flow reversal in a part of the film. In conjunction with the hydrodynamic stress, the Maxwell stress is also considered in the problem formulation. The electrokinetic potential at the liquid-air and solid-liquid interfaces is modelled by the Poisson-Boltzmann equation with the Debye-Hückel approximation. The O-S analysis shows a finite-wavenumber shear mode of instability when the inertial forces are strong and an LW interfacial mode of instability in the regime where the viscous force dominates. Interestingly, both the modes are found to form beyond a critical flow rate. The shear (interfacial) mode is found to be dominant when the film is thick (thin), the electric field applied is strong (weak), and the zeta-potentials on the liquid-air and solid-liquid interfaces are high (small). The LW analysis predicts the presence of the interfacial mode, but fails to capture the shear mode. The change in the propagation direction of the interfacial mode with the zeta-potential is predicted by both O-S and LW analyses. The parametric range in which the LW analysis is valid is thus demonstrated. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The use of clamping grips and friction pads by tree frogs for climbing curved surfaces

PubMed Central

Ji, Aihong; Yuan, Shanshan; Hill, Iain; Wang, Huan; Barnes, W. Jon P.; Dai, Zhendong; Sitti, Metin

2017-01-01

Most studies on the adhesive mechanisms of climbing animals have addressed attachment against flat surfaces, yet many animals can climb highly curved surfaces, like twigs and small branches. Here we investigated whether tree frogs use a clamping grip by recording the ground reaction forces on a cylindrical object with either a smooth or anti-adhesive, rough surface. Furthermore, we measured the contact area of fore and hindlimbs against differently sized transparent cylinders and the forces of individual pads and subarticular tubercles in restrained animals. Our study revealed that frogs use friction and normal forces of roughly a similar magnitude for holding on to cylindrical objects. When challenged with climbing a non-adhesive surface, the compressive forces between opposite legs nearly doubled, indicating a stronger clamping grip. In contrast to climbing flat surfaces, frogs increased the contact area on all limbs by engaging not just adhesive pads but also subarticular tubercles on curved surfaces. Our force measurements showed that tubercles can withstand larger shear stresses than pads. SEM images of tubercles revealed a similar structure to that of toe pads including the presence of nanopillars, though channels surrounding epithelial cells were less pronounced. The tubercles' smaller size, proximal location on the toes and shallow cells make them probably less prone to buckling and thus ideal for gripping curved surfaces. PMID:28228509

Molecular-scale shear response of the organic semiconductor β -DBDCS (100) surface

NASA Astrophysics Data System (ADS)

Álvarez-Asencio, Rubén; Moreno-Ramírez, Jorge S.; Pimentel, Carlos; Casado, Santiago; Matta, Micaela; Gierschner, Johannes; Muccioli, Luca; Yoon, Seong-Jun; Varghese, Shinto; Park, Soo Young; Gnecco, Enrico; Pina, Carlos M.

2017-09-01

In this work we present friction-force microscopy (FFM) lattice-resolved images acquired on the (100) facet of the semiconductor organic oligomer (2 Z ,2'Z )-3 , 3' -(1,4-phenylene)bis(2-(4-butoxyphenyl)acrylonitrile) (β -DBDCS) crystal in water at room temperature. Stick-slip contrast, lateral contact stiffness, and friction forces are found to depend strongly on the sliding direction due to the anisotropic packing of the molecular chains forming the crystal surface along the [010] and [001] directions. The anisotropy also causes the maximum value of the normal force applicable before wearing to increase by a factor of 3 when the scan is performed along the [001] direction on the (100) face. Altogether, our results contribute to achieving a better understanding of the molecular origin of friction anisotropy on soft crystalline surfaces, which has been often hypothesized but rarely investigated in the literature.

Rheological State Diagrams for Rough Colloids in Shear Flow.

PubMed

Hsiao, Lilian C; Jamali, Safa; Glynos, Emmanouil; Green, Peter F; Larson, Ronald G; Solomon, Michael J

2017-10-13

To assess the role of particle roughness in the rheological phenomena of concentrated colloidal suspensions, we develop model colloids with varying surface roughness length scales up to 10% of the particle radius. Increasing surface roughness shifts the onset of both shear thickening and dilatancy towards lower volume fractions and critical stresses. Experimental data are supported by computer simulations of spherical colloids with adjustable friction coefficients, demonstrating that a reduction in the onset stress of thickening and a sign change in the first normal stresses occur when friction competes with lubrication. In the quasi-Newtonian flow regime, roughness increases the effective packing fraction of colloids. As the shear stress increases and suspensions of rough colloids approach jamming, the first normal stresses switch signs and the critical force required to generate contacts is drastically reduced. This is likely a signature of the lubrication films giving way to roughness-induced tangential interactions that bring about load-bearing contacts in the compression axis of flow.

Rheological State Diagrams for Rough Colloids in Shear Flow

NASA Astrophysics Data System (ADS)

Hsiao, Lilian C.; Jamali, Safa; Glynos, Emmanouil; Green, Peter F.; Larson, Ronald G.; Solomon, Michael J.

2017-10-01

To assess the role of particle roughness in the rheological phenomena of concentrated colloidal suspensions, we develop model colloids with varying surface roughness length scales up to 10% of the particle radius. Increasing surface roughness shifts the onset of both shear thickening and dilatancy towards lower volume fractions and critical stresses. Experimental data are supported by computer simulations of spherical colloids with adjustable friction coefficients, demonstrating that a reduction in the onset stress of thickening and a sign change in the first normal stresses occur when friction competes with lubrication. In the quasi-Newtonian flow regime, roughness increases the effective packing fraction of colloids. As the shear stress increases and suspensions of rough colloids approach jamming, the first normal stresses switch signs and the critical force required to generate contacts is drastically reduced. This is likely a signature of the lubrication films giving way to roughness-induced tangential interactions that bring about load-bearing contacts in the compression axis of flow.

Effect of different surface treatments on the shear bond strength of nanofilled composite repairs

PubMed Central

Ahmadizenouz, Ghazaleh; Esmaeili, Behnaz; Taghvaei, Arnica; Jamali, Zahra; Jafari, Toloo; Amiri Daneshvar, Farshid; Khafri, Soraya

2016-01-01

Background. Repairing aged composite resin is a challenging process. Many surface treatment options have been proposed to this end. This study evaluated the effect of different surface treatments on the shear bond strength (SBS) of nano-filled composite resin repairs. Methods. Seventy-five cylindrical specimens of a Filtek Z350XT composite resin were fabricated and stored in 37°C distilled water for 24 hours. After thermocycling, the specimens were divided into 5 groups according to the following surface treatments: no treatment (group 1); air abrasion with 50-μm aluminum oxide particles (group 2); irradiation with Er:YAG laser beams (group 3); roughening with coarse-grit diamond bur + 35% phosphoric acid (group 4); and etching with 9% hydrofluoric acid for 120 s (group 5). Another group of Filtek Z350XT composite resin samples (4×6 mm) was fabricated for the measurement of cohesive strength (group 6). A silane coupling agent and an adhesive system were applied after each surface treatment. The specimens were restored with the same composite resin and thermocycled again. A shearing force was applied to the interface in a universal testing machine. Data were analyzed using one-way ANOVA and post hoc Tukey tests (P < 0.05). Results. One-way ANOVA indicated significant differences between the groups (P < 0.05). SBS of controls was significantly lower than the other groups; differences between groups 2, 3, 4, 5 and 6 were not significant. Surface treatment with diamond bur + 35% phosphoric acid resulted in the highest bond strength. Conclusion. All the surface treatments used in this study improved the shear bond strength of nanofilled composite resin used. PMID:27092209

Dynamic mortar finite element method for modeling of shear rupture on frictional rough surfaces

NASA Astrophysics Data System (ADS)

Tal, Yuval; Hager, Bradford H.

2017-09-01

This paper presents a mortar-based finite element formulation for modeling the dynamics of shear rupture on rough interfaces governed by slip-weakening and rate and state (RS) friction laws, focusing on the dynamics of earthquakes. The method utilizes the dual Lagrange multipliers and the primal-dual active set strategy concepts, together with a consistent discretization and linearization of the contact forces and constraints, and the friction laws to obtain a semi-smooth Newton method. The discretization of the RS friction law involves a procedure to condense out the state variables, thus eliminating the addition of another set of unknowns into the system. Several numerical examples of shear rupture on frictional rough interfaces demonstrate the efficiency of the method and examine the effects of the different time discretization schemes on the convergence, energy conservation, and the time evolution of shear traction and slip rate.

[Bond strength evaluation of four adhesive systems to dentin in vitro].

PubMed

Xiao, Ximei; Xing, Lu; Xu, Haiping; Jiang, Zhe; Su, Qin

2012-08-01

To compare the adhesive strength and observe the bonding interface. According to statistic analysis and scanning electron microscope (SEM) observation, the resistance capacity of four adhesive systems is evaluated. Prime & Bond NT (PBNT), Tetric N-Bond (TNB), Clearfil SE Bond (CSEB), G Bond (GB) were bonded to the occlusal surfaces and mesial surfaces of third molars respectively. The mesial resins received shear force experiment and the fracture load were recorded. The tensile bond strength (TBS) of the remaining parts were tested. The interfacial configuration were observed under SEM. In the shear bond strength (SBS) experiment, PBNT and TNB showed the best result, but there was no significant difference between them (P>0.05). The SBS of PBNT was stronger than that of CSEB and GB (P<0.05). The SBS of TNB was stronger than that of GB (P<0.05). There was no significant difference between TNB and CSEB (P>0.05). In accordance with the shear force result, the TBS of PBNT and TNB was larger than CSEB and GB (P<0.05). Under SEM, resin tags of PBNT and TNB were longer and slender, the bonding layer was thick. Resin tags of CSEB were shorter, the ones of GB were the fewest and shortest. Compared to self-etching system, total-etching system could reach better bonding strength. There is some connection between the interfacial configuration of adhesives and bond strength of them.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhu, Bo; Zhao, Hongwei, E-mail: hwzhao@jlu.edu.cn, E-mail: khl69@163.com; Zhao, Dan

It has always been a critical issue to understand the material removal behavior of Vibration-Assisted Machining (VAM), especially on atomic level. To find out the effects of vibration frequency on material removal response, a three-dimensional molecular dynamics (MD) model has been established in this research to investigate the effects of scratched groove, crystal defects on the surface quality, comparing with the Von Mises shear strain and tangential force in simulations during nano-scratching process. Comparisons are made among the results of simulations from different vibration frequency with the same scratching feed, depth, amplitude and crystal orientation. Copper potential in this simulationmore » is Embedded-Atom Method (EAM) potential. Interaction between copper and carbon atoms is Morse potential. Simulational results show that higher frequency can make groove smoother. Simulation with high frequency creates more dislocations to improve the machinability of copper specimen. The changing frequency does not have evident effects on Von Mises shear strain. Higher frequency can decrease the tangential force to reduce the consumption of cutting energy and tool wear. In conclusion, higher vibration frequency in VAM on mono-crystalline copper has positive effects on surface finish, machinablility and tool wear reduction.« less

Biomimetic wall-shaped hierarchical microstructure for gecko-like attachment.

PubMed

Kasem, Haytam; Tsipenyuk, Alexey; Varenberg, Michael

2015-04-21

Most biological hairy adhesive systems involved in locomotion rely on spatula-shaped terminal elements, whose operation has been actively studied during the last decade. However, though functional principles underlying their amazing performance are now well understood, due to technical difficulties in manufacturing the complex structure of hierarchical spatulate systems, a biomimetic surface structure featuring true shear-induced dynamic attachment still remains elusive. To try bridging this gap, a novel method of manufacturing gecko-like attachment surfaces is devised based on a laser-micromachining technology. This method overcomes the inherent disadvantages of photolithography techniques and opens wide perspectives for future production of gecko-like attachment systems. Advanced smart-performance surfaces featuring thin-film-based hierarchical shear-activated elements are fabricated and found capable of generating friction force of several tens of times the contact load, which makes a significant step forward towards a true gecko-like adhesive.

The effects of forcing on a single stream shear layer and its parent boundary layer

NASA Technical Reports Server (NTRS)

Haw, Richard C.; Foss, John F.

1990-01-01

Forcing and its effect on fluid flows has become an accepted tool in the study and control of flow systems. It has been used both as a diagnostic tool, to explore the development and interaction of coherent structures, and as a method of controlling the behavior of the flow. A number of forcing methods have been used in order to provide a perturbation to the flow; among these are the use of an oscillating trailing edge, acoustically driven slots, external acoustic forcing, and mechanical piston methods. The effect of a planar mechanical piston forcing on a single stream shear layer is presented; it can be noted that this is one of the lesser studied free shear layers. The single stream shear layer can be characterized by its primary flow velocity scale and the thickness of the separating boundary layer. The velocity scale is constant over the length of the flow field; theta (x) can be used as a width scale to characterize the unforced shear layer. In the case of the forced shear layer the velocity field is a function of phase time and definition of a width measure becomes somewhat problematic.

Observations and a model of undertow over the inner continental shelf

USGS Publications Warehouse

Lentz, Steven J.; Fewings, Melanie; Howd, Peter; Fredericks, Janet; Hathaway, Kent

2008-01-01

Onshore volume transport (Stokes drift) due to surface gravity waves propagating toward the beach can result in a compensating Eulerian offshore flow in the surf zone referred to as undertow. Observed offshore flows indicate that wave-driven undertow extends well offshore of the surf zone, over the inner shelves of Martha’s Vineyard, Massachusetts, and North Carolina. Theoretical estimates of the wave-driven offshore transport from linear wave theory and observed wave characteristics account for 50% or more of the observed offshore transport variance in water depths between 5 and 12 m, and reproduce the observed dependence on wave height and water depth.During weak winds, wave-driven cross-shelf velocity profiles over the inner shelf have maximum offshore flow (1–6 cm s−1) and vertical shear near the surface and weak flow and shear in the lower half of the water column. The observed offshore flow profiles do not resemble the parabolic profiles with maximum flow at middepth observed within the surf zone. Instead, the vertical structure is similar to the Stokes drift velocity profile but with the opposite direction. This vertical structure is consistent with a dynamical balance between the Coriolis force associated with the offshore flow and an along-shelf “Hasselmann wave stress” due to the influence of the earth’s rotation on surface gravity waves. The close agreement between the observed and modeled profiles provides compelling evidence for the importance of the Hasselmann wave stress in forcing oceanic flows. Summer profiles are more vertically sheared than either winter profiles or model profiles, for reasons that remain unclear.

Interpretation of the human skin biotribological behaviour after tape stripping

PubMed Central

Pailler-Mattei, C.; Guerret-Piécourt, C.; Zahouani, H.; Nicoli, S.

2011-01-01

The present study deals with the modification of the human skin biotribological behaviour after tape stripping. The tape-stripping procedure consists in the sequential application and removal of adhesive tapes on the skin surface in order to remove stratum corneum (SC) layers, which electrically charges the skin surface. The skin electric charges generated by tape stripping highly change the skin friction behaviour by increasing the adhesion component of the skin friction coefficient. It has been proposed to rewrite the friction adhesion component as the sum of two terms: the first classical adhesion term depending on the intrinsic shear strength, τ0, and the second term depending on the electric shear strength, τelec. The experimental results allowed to estimate a numerical value of the electric shear strength τelec. Moreover, a plan capacitor model with a dielectric material inside was used to modelize the experimental system. This physical model permitted to evaluate the friction electric force and the electric shear strength values to calculate the skin friction coefficient after the tape stripping. The comparison between the experimental and the theoretical value of the skin friction coefficient after the tape stripping has shown the importance of the electric charges on skin biotribological behaviour. The static electric charges produced by tape stripping on the skin surface are probably able to highly modify the interaction of formulations with the skin surface and their spreading properties. This phenomenon, generally overlooked, should be taken into consideration as it could be involved in alteration of drug absorption. PMID:21227961

Interpretation of the human skin biotribological behaviour after tape stripping.

PubMed

Pailler-Mattei, C; Guerret-Piécourt, C; Zahouani, H; Nicoli, S

2011-07-06

The present study deals with the modification of the human skin biotribological behaviour after tape stripping. The tape-stripping procedure consists in the sequential application and removal of adhesive tapes on the skin surface in order to remove stratum corneum (SC) layers, which electrically charges the skin surface. The skin electric charges generated by tape stripping highly change the skin friction behaviour by increasing the adhesion component of the skin friction coefficient. It has been proposed to rewrite the friction adhesion component as the sum of two terms: the first classical adhesion term depending on the intrinsic shear strength, τ(0), and the second term depending on the electric shear strength, τ(elec). The experimental results allowed to estimate a numerical value of the electric shear strength τ(elec). Moreover, a plan capacitor model with a dielectric material inside was used to modelize the experimental system. This physical model permitted to evaluate the friction electric force and the electric shear strength values to calculate the skin friction coefficient after the tape stripping. The comparison between the experimental and the theoretical value of the skin friction coefficient after the tape stripping has shown the importance of the electric charges on skin biotribological behaviour. The static electric charges produced by tape stripping on the skin surface are probably able to highly modify the interaction of formulations with the skin surface and their spreading properties. This phenomenon, generally overlooked, should be taken into consideration as it could be involved in alteration of drug absorption.

The Role of Water in Mediating Interfacial Adhesion and Shear Strength in Graphene Oxide.

PubMed

Soler-Crespo, Rafael A; Gao, Wei; Mao, Lily; Nguyen, Hoang T; Roenbeck, Michael R; Paci, Jeffrey T; Huang, Jiaxing; Nguyen, SonBinh T; Espinosa, Horacio D

2018-06-12

Graphene oxide (GO), whose highly tunable surface chemistry enables the formation of strong interfacial hydrogen-bond networks, has garnered increasing interest in the design of devices that operate in the presence of water. For instance, previous studies have suggested that controlling GO's surface chemistry leads to enhancements in interfacial shear strength, allowing engineers to manage deformation pathways and control failure mechanisms. However, these previous reports have not explored the role of ambient humidity and only offer extensive chemical modifications to GO's surface as the main pathway to control GO's interfacial properties. Herein, through atomic force microscopy experiments on GO-GO interfaces, the adhesion energy and interfacial shear strength of GO were measured as a function of ambient humidity. Experimental evidence shows that adhesion energy and interfacial shear strength can be improved by a factor of 2-3 when GO is exposed to moderate (∼30% water weight) water content. Furthermore, complementary molecular dynamics simulations uncovered the mechanisms by which these nanomaterial interfaces achieve their properties. They reveal that the strengthening mechanism arises from the formation of strongly interacting hydrogen-bond networks, driven by the chemistry of the GO basal plane and intercalated water molecules between two GO surfaces. In summary, the methodology and findings here reported provide pathways to simultaneously optimize GO's interfacial and in-plane mechanical properties, by tailoring the chemistry of GO and accounting for water content, in engineering applications such as sensors, filtration membranes, wearable electronics, and structural materials.

The flares of August 1972

NASA Technical Reports Server (NTRS)

Zirin, H.; Tanaka, K.

1972-01-01

Analysis is made of observations of the August, 1972 flares at Big Bear and Tel Aviv, involving monochromatic movies, magnetograms, and spectra. In each flare the observations fit a model of particle acceleration in the chromosphere with emission produced by impart and by heating by the energetic electrons and protons. The region showed twisted flux and high gradients from birth, and flares appear due to strong magnetic shears and gradients across the neutral line produced by sunspot motions. Post flare loops show a strong change from sheared, force-free fields parallel to potential-field-like loops, perpendicular to the neutral line above the surface.

Structural properties of impact ices accreted on aircraft structures

NASA Technical Reports Server (NTRS)

Scavuzzo, R. J.; Chu, M. L.

1987-01-01

The structural properties of ice accretions formed on aircraft surfaces are studied. The overall objectives are to measure basic structural properties of impact ices and to develop finite element analytical procedures for use in the design of all deicing systems. The Icing Research Tunnel (IRT) was used to produce simulated natural ice accretion over a wide range of icing conditions. Two different test apparatus were used to measure each of the three basic mechanical properties: tensile, shear, and peeling. Data was obtained on both adhesive shear strength of impact ices and peeling forces for various icing conditions. The influences of various icing parameters such as tunnel air temperature and velocity, icing cloud drop size, material substrate, surface temperature at ice/material interface, and ice thickness were studied. A finite element analysis of the shear test apparatus was developed in order to gain more insight in the evaluation of the test data. A comparison with other investigators was made. The result shows that the adhesive shear strength of impact ice typically varies between 40 and 50 psi, with peak strength reaching 120 psi and is not dependent on the kind of substrate used, the thickness of accreted ice, and tunnel temperature below 4 C.

Simulation of cemented granular materials. I. Macroscopic stress-strain response and strain localization.

PubMed

Estrada, Nicolas; Lizcano, Arcesio; Taboada, Alfredo

2010-07-01

This is the first of two papers investigating the mechanical response of cemented granular materials by means of contact dynamics simulations. In this paper, a two-dimensional polydisperse sample with high-void ratio is constructed and then sheared in a simple shear numerical device at different confinement levels. We study the macroscopic response of the material in terms of mean and deviatoric stresses and strains. We show that the introduction of a local force scale, i.e., the tensile strength of the cemented bonds, causes the material to behave in a rigid-plastic fashion, so that a yield surface can be easily determined. This yield surface has a concave-down shape in the mean:deviatoric stress plane and it approaches a straight line, i.e., a Coulomb strength envelope, in the limit of a very dense granular material. Beyond yielding, the cemented structure gradually degrades until the material eventually behaves as a cohesionless granular material. Strain localization is also investigated, showing that the strains concentrate in a shear band whose thickness increases with the confining stress. The void ratio inside the shear band at the steady state is shown to be a material property that depends only on contact parameters.

Neuronal activity in somatosensory cortex related to tactile exploration

PubMed Central

Fortier-Poisson, Pascal

2015-01-01

The very light contact forces (∼0.60 N) applied by the fingertips during tactile exploration reveal a clearly optimized sensorimotor strategy. To investigate the cortical mechanisms involved with this behavior, we recorded 230 neurons in the somatosensory cortex (S1), as two monkeys scanned different surfaces with the fingertips in search of a tactile target without visual feedback. During the exploration, the monkeys, like humans, carefully controlled the finger forces. High-friction surfaces offering greater tangential shear force resistance to the skin were associated with decreased normal contact forces. The activity of one group of neurons was modulated with either the normal or tangential force, with little or no influence from the orthogonal force component. A second group responded to kinetic friction or the ratio of tangential to normal forces rather than responding to a specific parameter, such as force magnitude or direction. A third group of S1 neurons appeared to respond to particular vectors of normal and tangential force on the skin. Although 45 neurons correlated with scanning speed, 32 were also modulated by finger forces, suggesting that forces on the finger should be considered as the primary parameter encoding the skin compliance and that finger speed is a secondary parameter that co-varies with finger forces. Neurons (102) were also tested with different textures, and the activity of 62 of these increased or decreased in relation to the surface friction. PMID:26467519

Shear forces in the contact patch of a braked-racing tyre

NASA Astrophysics Data System (ADS)

Gruber, Patrick; Sharp, Robin S.

2012-12-01

This article identifies tyre modelling features that are fundamental to the accurate simulation of the shear forces in the contact patch of a steady-rolling, slipping and cambered racing tyre. The features investigated include contact patch shape, contact pressure distribution, carcass flexibility, rolling radius (RR) variations and friction coefficient. Using a previously described physical tyre model of modular nature, validated for static conditions, the influence of each feature on the shear forces generated is examined under different running conditions, including normal loads of 1500, 3000 and 4500 N, camber angles of 0° and-3°, and longitudinal slip ratios from 0 to-20%. Special attention is paid to heavy braking, in which context the aligning moment is of great interest in terms of its connection with the limit-handling feel. The results of the simulations reveal that true representations of the contact patch shape, carcass flexibility and lateral RR variation are essential for an accurate prediction of the distribution and the magnitude of the shear forces generated at the tread-road interface of the cambered tyre. Independent of the camber angle, the contact pressure distribution primarily influences the shear force distribution and the slip characteristics around the peak longitudinal force. At low brake-slip ratios, the friction coefficient affects the shear forces in terms of their distribution, while, at medium to high-slip ratios, the force magnitude is significantly affected. On the one hand, these findings help in the creation of efficient yet accurate tyre models. On the other hand, the research results allow improved understanding of how individual tyre components affect the generation of shear forces in the contact patch of a rolling and slipping tyre.

Increasing hip and knee flexion during a drop-jump task reduces tibiofemoral shear and compressive forces: implications for ACL injury prevention training.

PubMed

Tsai, Liang-Ching; Ko, Yi-An; Hammond, Kyle E; Xerogeanes, John W; Warren, Gordon L; Powers, Christopher M

2017-12-01

Although most ACL injury prevention programmes encourage greater hip and knee flexion during landing, it remains unknown how this technique influences tibiofemoral joint forces. We examined whether a landing strategy utilising greater hip and knee flexion decreases tibiofemoral anterior shear and compression. Twelve healthy women (25.9 ± 3.5 years) performed a drop-jump task before and after a training session (10-15 min) that emphasised greater hip and knee flexion. Peak tibiofemoral anterior shear and compressive forces were calculated using an electromyography (EMG)-driven knee model that incorporated joint kinematics, EMG and participant-specific muscle volumes and patella tendon orientation measured using magnetic resonance imaging (MRI). Participants demonstrated a decrease in peak anterior tibial shear forces (11.1 ± 3.3 vs. 9.6 ± 2.7 N · kg -1 ; P = 0.008) and peak tibiofemoral compressive forces (68.4 ± 7.6 vs. 62.0 ± 5.5 N · kg -1 ; P = 0.015) post-training. The decreased peak anterior tibial shear was accompanied by a decrease in the quadriceps anterior shear force, while the decreased peak compressive force was accompanied by decreased ground reaction force and hamstring forces. Our data provide justification for injury prevention programmes that encourage greater hip and knee flexion during landing to reduce tibiofemoral joint loading.

Modeling Shear Induced Von Willebrand Factor Binding to Collagen

NASA Astrophysics Data System (ADS)

Dong, Chuqiao; Wei, Wei; Morabito, Michael; Webb, Edmund; Oztekin, Alparslan; Zhang, Xiaohui; Cheng, Xuanhong

2017-11-01

Von Willebrand factor (vWF) is a blood glycoprotein that binds with platelets and collagen on injured vessel surfaces to form clots. VWF bioactivity is shear flow induced: at low shear, binding between VWF and other biological entities is suppressed; for high shear rate conditions - as are found near arterial injury sites - VWF elongates, activating its binding with platelets and collagen. Based on parameters derived from single molecule force spectroscopy experiments, we developed a coarse-grain molecular model to simulate bond formation probability as a function of shear rate. By introducing a binding criterion that depends on the conformation of a sub-monomer molecular feature of our model, the model predicts shear-induced binding, even for conditions where binding is highly energetically favorable. We further investigate the influence of various model parameters on the ability to predict shear-induced binding (vWF length, collagen site density and distribution, binding energy landscape, and slip/catch bond length) and demonstrate parameter ranges where the model provides good agreement with existing experimental data. Our results may be important for understanding vWF activity and also for achieving targeted drug therapy via biomimetic synthetic molecules. National Science Foundation (NSF),Division of Mathematical Sciences (DMS).

Impact of Acoustic Radiation Force Excitation Geometry on Shear Wave Dispersion and Attenuation Estimates.

PubMed

Lipman, Samantha L; Rouze, Ned C; Palmeri, Mark L; Nightingale, Kathryn R

2018-04-01

Shear wave elasticity imaging (SWEI) characterizes the mechanical properties of human tissues to differentiate healthy from diseased tissue. Commercial scanners tend to reconstruct shear wave speeds for a region of interest using time-of-flight methods reporting a single shear wave speed (or elastic modulus) to the end user under the assumptions that tissue is elastic and shear wave speeds are not dependent on the frequency content of the shear waves. Human tissues, however, are known to be viscoelastic, resulting in dispersion and attenuation. Shear wave spectroscopy and spectral methods have been previously reported in the literature to quantify shear wave dispersion and attenuation, commonly making an assumption that the acoustic radiation force excitation acts as a cylindrical source with a known geometric shear wave amplitude decay. This work quantifies the bias in shear dispersion and attenuation estimates associated with making this cylindrical wave assumption when applied to shear wave sources with finite depth extents, as commonly occurs with realistic focal geometries, in elastic and viscoelastic media. Bias is quantified using analytically derived shear wave data and shear wave data generated using finite-element method models. Shear wave dispersion and attenuation bias (up to 15% for dispersion and 41% for attenuation) is greater for more tightly focused acoustic radiation force sources with smaller depths of field relative to their lateral extent (height-to-width ratios <16). Dispersion and attenuation errors associated with assuming a cylindrical geometric shear wave decay in SWEI can be appreciable and should be considered when analyzing the viscoelastic properties of tissues with acoustic radiation force source distributions with limited depths of field. Copyright © 2018 World Federation for Ultrasound in Medicine and Biology. Published by Elsevier Inc. All rights reserved.

Airflow in Gravity Sewers - Determination of Wastewater Drag Coefficient.

PubMed

Bentzen, Thomas Ruby; Østertoft, Kristian Kilsgaard; Vollertsen, Jes; Fuglsang, Emil Dietz; Nielsen, Asbjørn Haaning

2016-03-01

Several experiments have been conducted in order to improve the understanding of the wastewater drag and the wall frictional force acting on the headspace air in gravity sewers. The aim of the study is to improve the data basis for a numerical model of natural sewer ventilation. The results of the study shows that by integrating the top/side wall shear stresses the log-law models for the air velocity distribution along the unwetted perimeter resulted in a good agreement with the friction forces calculated by use of the Colebrook-White formula for hydraulic smooth pipes. Secondly, the water surface drags were found by log-law models of the velocity distribution in turbulent flows to fit velocity profiles measured from the water surface and by integrating the water surface drags along the wetted perimeter, mean water surface drags were found and a measure of the water surface drag coefficient was found.

Ocean Surface Observations of the Diurnal Cycle of Turbulence with ASIP

NASA Astrophysics Data System (ADS)

Ward, Brian; Sutherland, Graig; Reverdin, Gilles; Marie, Louis; Christensen, Kai; Brostrom, Goran; Harcourt, Ramsey; Breivik, Oyvind

2015-04-01

The STRASSE field experiment was conducted in August/September 2012 as part of the Salinity Processes in the Upper Ocean Regional Study (SPURS) campaign. The average conditions during STRASSE were low wind and high insolation, which are typical for the generation of near-surface diurnal warming. We deployed the Air-Sea Interaction Profiler (ASIP), an autonomous upwardly-rising microstructure instrument capable of resolving small-scale processes close to the air-sea interface. ASIP provides direct estimates of the dissipation rate of turbulent kinetic energy, temperature, salinity, and PAR at timescales suitable for the study of diurnal processes. In combination with the ASIP data, we had shipboard meteorological data for calculation of atmospheric forcing, and a surface mounted Lagrangian ADCP for determination of the near-surface velocity. There was a strong diurnal cycle of temperature and dissipation (from ASIP) and shear (from an ADCP). As air-sea fluxes are driven by turbulence immediately at the air-sea interface, the presence of this enhanced shear-induced turbulence will enhance fluxes.

Effects of Combined Shear and Thermal Forces on Destruction of Microbacterium lacticum

PubMed Central

Bulut, S.; Waites, W. M.; Mitchell, J. R.

1999-01-01

A twin-screw extruder and a rotational rheometer were used to generate shear forces in concentrated gelatin inoculated with a heat-resistant isolate of a vegetative bacterial species, Microbacterium lacticum. Shear forces in the extruder were mainly controlled by varying the water feed rate. The water content of the extrudates changed between 19 and 45% (wet weight basis). Higher shear forces generated at low water contents and the calculated die wall shear stress correlated strongly with bacterial destruction. No surviving microorganisms could be detected at the highest wall shear stress of 409 kPa, giving log reduction of 5.3 (minimum detection level, 2 × 104 CFU/sample). The mean residence time of the microorganism in the extruder was 49 to 58 s, and the maximum temperature measured in the end of the die was 73°C. The D75°C of the microorganism in gelatin at 65% water content was 20 min. It is concluded that the physical forces generated in the reverse screw element and the extruder die rather than heat played a major part in cell destruction. In a rotational rheometer, after shearing of a mix of microorganisms with gelatin at 65% (wt/wt) moisture content for 4 min at a shear stress of 2.8 kPa and a temperature of 75°C, the number of surviving microorganisms in the sheared sample was 5.2 × 106 CFU/g of sample compared with 1.4 × 108 CFU/g of sample in the nonsheared control. The relative effectiveness of physical forces in the killing of bacteria and destruction of starch granules is discussed. PMID:10508076

The influence of bone damage on press-fit mechanics.

PubMed

Bishop, Nicholas E; Höhn, Jan-Christian; Rothstock, Stephan; Damm, Niklas B; Morlock, Michael M

2014-04-11

Press-fitting is used to anchor uncemented implants in bone. It relies in part on friction resistance to relative motion at the implant-bone interface to allow bone ingrowth and long-term stability. Frictional shear capacity is related to the interference fit of the implant and the roughness of its surface. It was hypothesised here that a rough implant could generate trabecular bone damage during implantation, which would reduce its stability. A device was constructed to simulate implantation by displacement of angled platens with varying surface finishes (polished, beaded and flaked) onto the surface of an embedded trabecular bone cube, to different nominal interferences. Push-in (implantation) and Pull-out forces were measured and micro-CT scans were made before and after testing to assess permanent bone deformation. Depth of permanent trabecular bone deformation ('damage'), Pull-out force and Radial force all increased with implantation displacement and with implantation force, for all surface roughnesses. The proposed hypothesis was rejected, since primary stability did not decrease with trabecular bone damage. In fact, Pull-out force linearly increased with push-in force, independently of trabecular bone damage or implant surface. This similar behaviour for the different surfaces might be explained by the compaction of bone into the surfaces during push-in so that Pull-out resistance is governed by bone-on-bone, rather than implant surface-on-bone friction. The data suggest that maximum stability is achieved for the maximum implantation force possible (regardless of trabecular bone damage or surface roughness), but this must be limited to prevent periprosthetic cortical bone fracture, patient damage and component malpositioning. Copyright © 2014 Elsevier Ltd. All rights reserved.

Rheological and Tribological Properties of Complex Biopolymer Solutions

NASA Astrophysics Data System (ADS)

Klossner, Rebecca Reese

2011-12-01

The rheological and tribological properties of an experimental synovial fluid model were investigated in order to determine the solution dynamics of the three most abundant macromolecules present in synovial fluid, the fluid that lubricates freely moving (synovial) joints. These components, hyaluronic acid (HA) and the plasma proteins, albumin and gamma-globulins are combined in a phosphate buffered saline solution (PBS) and subjected to steady shear rheology testing, as well as nanoindenter-based scratch testing, which allows for the study of the lubrication properties of the experimental synovial fluid model. Steady shear experiments, where the shear rate was increased from low to high, and then decreased from high to low, showed hysteresis in only protein containing solutions, whereas samples of HA in PBS behaved as a "typical" polyelectrolyte in solution. Subsequent rheological experiments on the synovial fluid model exhibited an increase in viscosity at low shear stresses, indicating that a structure was present at these low shear stresses, which was not found at higher shear stresses. This result is in agreement with studies conducted on the same model which show unusual rheological behavior at low shear rates. Low shear stresses can cause modifications to the external protein surface, resulting in their unfolding and creating many opportunities for the molecules to reorder themselves. As the proteins reorder themselves, the newly exposed hydrophobic patches will have a tendency to aggregate together, creating a network within the fluid, and, in turn causing the observed increased viscosity at low shear stresses. Additionally, an anti-inflammatory drug, hydroxychloroquine (HCQ) was added to the solutions. This addition diminishes the protein aggregation process substantially. Finally, the HA component of the synovial fluid model was replaced with a neutral polymer in order to examine the role of HA in synovial fluid. As suspected, the HA appears to have little impact on the actual aggregation process. Additionally, the relationship between the rheology and tribology of the SFM was studied through a series of nanoscratch tests using a Hysitron nanoindenter. The nanoindenter has the ability to measure both normal and lateral forces simultaneously, which gives an indication of the lubricity of the solution. The coefficient of friction values for solutions of varying protein concentrations were determined by dividing the lateral force by the normal force. Tribological testing of the synovial fluid model and modified solutions were carried out on spin-cast polyethylene and ultra high molecular weight polyethylene sheets. At lower molecular weight substrates, the film thickness limited the validity of the generated data, and with higher molecular weight surfaces, surface roughness effects were found to dominate the tribological response. Finally, the addition of HCQ does not have a large impact on the tribological data, indicating that the anti-inflammatory drug does not significantly impact the lubrication properties within the synovial fluid model. Finally, additional rheological studies of biopolymer solutions were conducted in which solutions containing chitosan, a natural, bioactive polymer, were characterized to determine their fitness for the electropsinning process. Chitosan fibers are difficult to electrospin, and through these studies, the entanglement concentration, a critical parameter for electrospinning, was determined. The generated rheological data provided a means to predict the morphology of the resulting nanofibers, and aspects of the difficulty in electrospinning chitosan were revealed.

Exploratory Experiments in the Tribological Behavior of Engineering Surfaces with Nano-Coating Using a Tribo-Rheometer

DTIC Science & Technology

2008-05-30

Tribological behavior and graphitization of carbon nanotubes grown on 440C stainless steel . Tribo. Lett., 19(2):119-125, 2005. D-2 ...with a stainless steel parallel plate configuration as shown in figure 1. Due to the radial variation of the local shear stress T in the parallel...using a force transducer that is mounted below the surface. B-1 Exploded View Stainless Steel Plate Lower Fixture Microscale View Figure 1:

Catch bonds govern adhesion through L-selectin at threshold shear.

PubMed

Yago, Tadayuki; Wu, Jianhua; Wey, C Diana; Klopocki, Arkadiusz G; Zhu, Cheng; McEver, Rodger P

2004-09-13

Flow-enhanced cell adhesion is an unexplained phenomenon that might result from a transport-dependent increase in on-rates or a force-dependent decrease in off-rates of adhesive bonds. L-selectin requires a threshold shear to support leukocyte rolling on P-selectin glycoprotein ligand-1 (PSGL-1) and other vascular ligands. Low forces decrease L-selectin-PSGL-1 off-rates (catch bonds), whereas higher forces increase off-rates (slip bonds). We determined that a force-dependent decrease in off-rates dictated flow-enhanced rolling of L-selectin-bearing microspheres or neutrophils on PSGL-1. Catch bonds enabled increasing force to convert short-lived tethers into longer-lived tethers, which decreased rolling velocities and increased the regularity of rolling steps as shear rose from the threshold to an optimal value. As shear increased above the optimum, transitions to slip bonds shortened tether lifetimes, which increased rolling velocities and decreased rolling regularity. Thus, force-dependent alterations of bond lifetimes govern L-selectin-dependent cell adhesion below and above the shear optimum. These findings establish the first biological function for catch bonds as a mechanism for flow-enhanced cell adhesion.

On the role of constant-stress surfaces in the problem of minimizing elastic stress concentration

NASA Technical Reports Server (NTRS)

Wheeler, L.

1976-01-01

Cases involving antiplane shear deformation, axisymmetric torsion, and plane strain theory, with surfaces of constant stress magnitude optimal in terms of minimizing stress, are investigated. Results for the plane theory refer to exterior doubly connected domains. Stresses generated by torsion of an elastic solid lying within a radially convex region of revolution with plane ends, body force absent, and lateral surface traction-free, are examined. The unknown portion of the boundary of such domains may involve a hole, fillet, or notch.

Calculation of zero-offset vertical seismic profiles generated by a horizontal point force acting on the surface of an elastic half-space

USGS Publications Warehouse

Hsi-Ping, Liu

1990-01-01

Impulse responses including near-field terms have been obtained in closed form for the zero-offset vertical seismic profiles generated by a horizontal point force acting on the surface of an elastic half-space. The method is based on the correspondence principle. Through transformation of variables, the Fourier transform of the elastic impulse response is put in a form such that the Fourier transform of the corresponding anelastic impulse response can be expressed as elementary functions and their definite integrals involving distance angular frequency, phase velocities, and attenuation factors. These results are used for accurate calculation of shear-wave arrival rise times of synthetic seismograms needed for data interpretation of anelastic-attenuation measurements in near-surface sediment. -Author

Interactions of hyaluronan grafted on protein surfaces studied using a quartz crystal microbalance and a surface force balance.

PubMed

Jiang, Lei; Han, Juan; Yang, Limin; Ma, Hongchao; Huang, Bo

2015-10-07

Vocal folds are complex and multilayer-structured where the main layer is widely composed of hyaluronan (HA). The viscoelasticity of HA is key to voice production in the vocal fold as it affects the initiation and maintenance of phonation. In this study a simple layer-structured surface model was set up to mimic the structure of the vocal folds. The interactions between two opposing surfaces bearing HA were measured and characterised to analyse HA's response to the normal and shear compression at a stress level similar to that in the vocal fold. From the measurements of the quartz crystal microbalance, atomic force microscopy and the surface force balance, the osmotic pressure, normal interactions, elasticity change, volume fraction, refractive index and friction of both HA and the supporting protein layer were obtained. These findings may shed light on the physical mechanism of HA function in the vocal fold and the specific role of HA as an important component in the effective treatment of the vocal fold disease.

Mechanical Activation of a Multimeric Adhesive Protein Through Domain Conformational Change

NASA Astrophysics Data System (ADS)

Wijeratne, Sithara S.; Botello, Eric; Yeh, Hui-Chun; Zhou, Zhou; Bergeron, Angela L.; Frey, Eric W.; Patel, Jay M.; Nolasco, Leticia; Turner, Nancy A.; Moake, Joel L.; Dong, Jing-fei; Kiang, Ching-Hwa

2013-03-01

The mechanical force-induced activation of the adhesive protein von Willebrand factor (VWF), which experiences high hydrodynamic forces, is essential in initiating platelet adhesion. The importance of the mechanical force-induced functional change is manifested in the multimeric VWF’s crucial role in blood coagulation, when high fluid shear stress activates plasma VWF (PVWF) multimers to bind platelets. Here, we showed that a pathological level of high shear stress exposure of PVWF multimers results in domain conformational changes, and the subsequent shifts in the unfolding force allow us to use force as a marker to track the dynamic states of the multimeric VWF. We found that shear-activated PVWF multimers are more resistant to mechanical unfolding than nonsheared PVWF multimers, as indicated in the higher peak unfolding force. These results provide insight into the mechanism of shear-induced activation of PVWF multimers.

Vacuum scanning capillary photoemission microscopy.

PubMed

Aseyev, S A; Cherkun, A P; Mironov, B N; Petrunin, V V; Chekalin, S V

2017-08-01

We demonstrate the use of a conical capillary in a scanning probe microscopy for surface analysis. The probe can measure photoemission from a substrate by transmitting photoelectrons along the capillary as a function of probe position. The technique is demonstrated on a model substrate consisting of a gold reflecting layer on a compact disc which has been illuminated by an unfocused laser beam with a wavelength 400nm, from a femtosecond laser with a beam size of 4mm. A quartz capillary with a 2-µm aperture has been used in the experiments. The period of gold microstructure, shown to be 1.6µ, was measured by the conical probe operating in shear force mode. In shear force regime, the dielectric capillary has been used as a "classical" SPM tip, which provided images reflecting the surface topology. In a photoelectron regime photoelectrons passed through hollow tip and entered a detector. The spatial distribution of the recorded photoelectrons consisted of periodic mountain-valley strips, resembling the surface profile of the sample. Submicron spatial resolution has been achieved. This approach paves the way to study pulsed photodesorption of large organic molecular ions with high spatial and element resolution using the combination of a hollow-tip scanner with time-of-flight technique. Copyright © 2017 Elsevier B.V. All rights reserved.

Shear Wave Imaging of Breast Tissue by Color Doppler Shear Wave Elastography.

PubMed

Yamakoshi, Yoshiki; Nakajima, Takahito; Kasahara, Toshihiro; Yamazaki, Mayuko; Koda, Ren; Sunaguchi, Naoki

2017-02-01

Shear wave elastography is a distinctive method to access the viscoelastic characteristic of the soft tissue that is difficult to obtain by other imaging modalities. This paper proposes a novel shear wave elastography [color Doppler shear wave imaging (CD SWI)] for breast tissue. Continuous shear wave is produced by a small lightweight actuator, which is attached to the tissue surface. Shear wave wavefront that propagates in tissue is reconstructed as a binary pattern that consists of zero and the maximum flow velocities on color flow image (CFI). Neither any modifications of the ultrasound color flow imaging instrument nor a high frame rate ultrasound imaging instrument is required to obtain the shear wave wavefront map. However, two conditions of shear wave displacement amplitude and shear wave frequency are needed to obtain the map. However, these conditions are not severe restrictions in breast imaging. This is because the minimum displacement amplitude is [Formula: see text] for an ultrasonic wave frequency of 12 MHz and the shear wave frequency is available from several frequencies suited for breast imaging. Fourier analysis along time axis suppresses clutter noise in CFI. A directional filter extracts shear wave, which propagates in the forward direction. Several maps, such as shear wave phase, velocity, and propagation maps, are reconstructed by CD SWI. The accuracy of shear wave velocity measurement is evaluated for homogeneous agar gel phantom by comparing with the acoustic radiation force impulse method. The experimental results for breast tissue are shown for a shear wave frequency of 296.6 Hz.

Factors that influence muscle shear modulus during passive stretch.

PubMed

Koo, Terry K; Hug, François

2015-09-18

Although elastography has been increasingly used for evaluating muscle shear modulus associated with age, sex, musculoskeletal, and neurological conditions, its physiological meaning is largely unknown. This knowledge gap may hinder data interpretation, limiting the potential of using elastography to gain insights into muscle biomechanics in health and disease. We derived a mathematical model from a widely-accepted Hill-type passive force-length relationship to gain insight about the physiological meaning of resting shear modulus of skeletal muscles under passive stretching, and validated the model by comparing against the ex-vivo animal data reported in our recent work (Koo et al. 2013). The model suggested that resting shear modulus of a slack muscle is a function of specific tension and parameters that govern the normalized passive muscle force-length relationship as well as the degree of muscle anisotropy. The model also suggested that although the slope of the linear shear modulus-passive force relationship is primarily related to muscle anatomical cross-sectional area (i.e. the smaller the muscle cross-sectional area, the more the increase in shear modulus to result in the same passive muscle force), it is also governed by the normalized passive muscle force-length relationship and the degree of muscle anisotropy. Taken together, although muscle shear modulus under passive stretching has a strong linear relationship with passive muscle force, its actual value appears to be affected by muscle's mechanical, material, and architectural properties. This should be taken into consideration when interpreting the muscle shear modulus values. Copyright © 2015 Elsevier Ltd. All rights reserved.

Biomechanics of leukocyte rolling

PubMed Central

Sundd, Prithu; Pospieszalska, Maria K.; Cheung, Luthur Siu-Lun; Konstantopoulos, Konstantinos; Ley, Klaus

2011-01-01

Leukocyte rolling on endothelial cells and other P-selectin substrates is mediated by P-selectin binding to P-selectin glycoprotein ligand-1 expressed on the tips of leukocyte microvilli. Leukocyte rolling is a result of rapid, yet balanced formation and dissociation of selectin-ligand bonds in the presence of hydrodynamic shear forces. The hydrodynamic forces acting on the bonds may either increase (catch bonds) or decrease (slip-bonds) their lifetimes. The force-dependent ‘catch-slip’ bond kinetics are explained using the ‘two pathway model’ for bond dissociation. Both the ‘sliding-rebinding’ and the ‘allosteric’ mechanisms attribute ‘catch-slip’ bond behavior to the force-induced conformational changes in the lectin-EGF domain hinge of selectins. Below a threshold shear stress, selectins cannot mediate rolling. This ‘shear-threshold’ phenomenon is a consequence of shear-enhanced tethering and catch-bond enhanced rolling. Quantitative dynamic footprinting microscopy has revealed that leukocytes rolling at venular shear stresses (> 0.6 Pa) undergo cellular deformation (large footprint) and form long tethers. The hydrodynamic shear force and torque acting on the rolling cell are thought to be synergistically balanced by the forces acting on tethers and stressed microvilli, however, their relative contribution remains to be determined. Thus, improvement beyond the current understanding requires in silico models that can predict both cellular and microvillus deformation and experiments that allow measurement of forces acting on individual microvilli and tethers. PMID:21515934

Imaging and characterizing shear wave and shear modulus under orthogonal acoustic radiation force excitation using OCT Doppler variance method.

PubMed

Zhu, Jiang; Qu, Yueqiao; Ma, Teng; Li, Rui; Du, Yongzhao; Huang, Shenghai; Shung, K Kirk; Zhou, Qifa; Chen, Zhongping

2015-05-01

We report on a novel acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE) technique for imaging shear wave and quantifying shear modulus under orthogonal acoustic radiation force (ARF) excitation using the optical coherence tomography (OCT) Doppler variance method. The ARF perpendicular to the OCT beam is produced by a remote ultrasonic transducer. A shear wave induced by ARF excitation propagates parallel to the OCT beam. The OCT Doppler variance method, which is sensitive to the transverse vibration, is used to measure the ARF-induced vibration. For analysis of the shear modulus, the Doppler variance method is utilized to visualize shear wave propagation instead of Doppler OCT method, and the propagation velocity of the shear wave is measured at different depths of one location with the M scan. In order to quantify shear modulus beyond the OCT imaging depth, we move ARF to a deeper layer at a known step and measure the time delay of the shear wave propagating to the same OCT imaging depth. We also quantitatively map the shear modulus of a cross-section in a tissue-equivalent phantom after employing the B scan.

Stress-Driven Melt Segregation and Organization in Partially Molten Rocks III: Annealing Experiments and Surface Tension-Driven Redistribution of Melt

NASA Astrophysics Data System (ADS)

Parsons, R.; Hustoft, J. W.; Holtzman, B. K.; Kohlstedt, D. L.; Phipps Morgan, J.

2004-12-01

As discussed in the two previous abstracts in this series, simple shear experiments on synthetic upper mantle-type rock samples reveal the segregation of melt into melt-rich bands separated by melt-depleted lenses. Here, we present new results from experiments designed to understand the driving forces working for and against melt segregation. To better understand the kinetics of surface tension-driven melt redistribution, we first deform samples at similar conditions (starting material, sample size, stress and strain) to produce melt-rich band networks that are statistically similar. Then the load is removed and the samples are statically annealed to allow surface tension to redistribute the melt-rich networks. Three samples of olivine + 20 vol% chromite + 4 vol% MORB were deformed at a confining pressure of 300 MPa and a temperature of 1523 K in simple shear at shear stresses of 20 - 55 MPa to shear strains of 3.5 and then statically annealed for 0, 10, or 100 h at the same P-T conditions. Melt-rich bands are fewer in number and appear more diffuse when compared to the deformed but not annealed samples. Bands with less melt tend to disappear more rapidly than more melt-rich ones. The melt fraction in the melt-rich bands decreased from 0.2 in the quenched sample to 0.1 in the sample annealed for 100 h. After deformation, the melt fraction in the melt-depleted regions are ~0.006; after static annealing for 100 h, this value increases to 0.02. These experiments provide new quantitative constraints on the kinetics of melt migration driven by surface tension. By quantifying this driving force in the same samples in which stress-driven distribution occurred, we learn about the relative kinetics of stress-driven melt segregation. The kinetics of both of these processes must be scaled together to mantle conditions to understand the importance of stress-driven melt segregation in the Earth, and to understand the interaction of this process with melt-rock reaction-driven processes.

Late-time mixing and turbulent behavior in high-energy-density shear experiments at high Atwood numbers

NASA Astrophysics Data System (ADS)

Flippo, Kirk

2017-10-01

The LANL Shear experiments on the NIF are designed to study the Kelvin-Helmholtz instability (KHI), which is the predominate mechanism for generating vorticity, leading to turbulence and mixing at high Reynolds numbers. The KHI is pervasive, as velocity sheared and density-stratified flows abound, from accretion disks of a black holes to the fuel capsule in an ICF implosion. The NIF laser has opened up a new class of long-lived planar HED fluid instability experiments that can scale fluid experiments over impressive orders of magnitude in pressure (up to > Mbar), temperature (>105 K) and space (<10s of μm) and still recover classical fluid instability behavior, and elucidate mixing and plasma effects. The reproducibility allows for the unique capability in an HED experiment to directly measure values comparable to those in the mix model, the Besnard-Harlow-Rauenzahn (BHR[3]) model implemented in the LANL hydro-code RAGE, like the mixedness parameter, b, and the turbulent kinetic energy using the observed coherent features. We have acquired time histories of 4 tracer materials and 3 surface finishes spanning dynamic Atwood numbers from 0.63 to 0.88 and developed Reynolds numbers around 106. When the shocks cross, the layer is exposed to extreme shear forces and evolves into KHI rollers from an unseeded (but naturally broadband) surface. Two sets of data are acquired for each material type: an edge-view and a plan-view, through the plane of the material. The results hint at plasma physics effects in the layer. The edge-view is compared to BHR calculations, to understand mixing and layer growth. The BHR model matches the evolution and asymptotic behavior of the layer, and the initial scale-length used for the model correlates well to initial surface roughness, even when the surface is artificially roughened, forcing the layer's evolution from coherent to disordered. This work performed under the auspices of the U.S. Department of Energy by LANL under contract DE-AC52-06NA25396.

Force and work to shear green southern pine logs at slow speed

Treesearch

Peter Koch

1971-01-01

When logs of three diameter classes and two specific gravity classes were sheared with a 3/8-inch-thick knife travelling at 2 inches per minute, shearing force and work averaged greatest for dense 13.6-inch logs cut with a knife having a 45o sharpness angle (73,517 pounds; 49,838 foot-pounds). Force and work averaged at least 5.1-inch bolts of...

Thermal Motion and Forced Migration of Colloidal Particles Generate Hydrostatic Pressure in Solvent

PubMed Central

Hammel, H. T.; Scholander, P. F.

1973-01-01

A colloidal solution of ferrite particles in an osmometer has been used to demonstrate that the property that propels water across the semipermeable membrane is the decrease in hydrostatic pressure in the water of the solution. A magnetic field gradient directed so as to force the ferrite particles away from the semipermeable membrane of the osmometer and toward the free surface of the solution enhanced the colloidal osmotic pressure. The enhancement of this pressure was always exactly equal to the augmentation of the pressure as measured by the outward force of the particles, against the area of the free surface. Contrariwise, directing the magnetic field gradient so as to force the ferrite particles away from the free surface and toward the semipermeable membrane diminished the colloidal osmotic pressure of the solution. For a sufficiently forceful field gradient, the initial colloidal osmotic pressure could be negative, followed by an equilibrium pressure approaching zero regardless of the force of the particles against the membrane. Thus, the osmotic pressure of a solution is to be attributed to the pressure in the solvent generated in opposition to the pressure of the solute particles caused by their interaction with the free surface (Brownian motion and/or an external field force), or by their viscous shear when they migrate through the solvent, or both. PMID:16592046

Multi-muscle synergies in an unusual postural task: quick shear force production.

PubMed

Robert, Thomas; Zatsiorsky, Vladimir M; Latash, Mark L

2008-05-01

We considered a hypothetical two-level hierarchy participating in the control of vertical posture. The framework of the uncontrolled manifold (UCM) hypothesis was used to explore the muscle groupings (M-modes) and multi-M-mode synergies involved in the stabilization of a time profile of the shear force in the anterior-posterior direction. Standing subjects were asked to produce pulses of shear force into a target using visual feedback while trying to minimize the shift of the center of pressure (COP). Principal component analysis applied to integrated muscle activation indices identified three M-modes. The composition of the M-modes was similar across subjects and the two directions of the shear force pulse. It differed from the composition of M-modes described in earlier studies of more natural actions associated with large COP shifts. Further, the trial-to-trial M-mode variance was partitioned into two components: one component that does not affect a particular performance variable (V(UCM)), and its orthogonal component (V(ORT)). We argued that there is a multi-M-mode synergy stabilizing this particular performance variable if V(UCM) is higher than V(ORT). Overall, we found a multi-M-mode synergy stabilizing both shear force and COP coordinate. For the shear force, this synergy was strong for the backward force pulses and nonsignificant for the forward pulses. An opposite result was found for the COP coordinate: the synergy was stronger for the forward force pulses. The study shows that M-mode composition can change in a task-specific way and that two different performance variables can be stabilized using the same set of elemental variables (M-modes). The different dependences of the ΔV indices for the shear force and COP coordinate on the force pulse direction supports applicability of the principle of superposition (separate controllers for different performance variables) to the control of different mechanical variables in postural tasks. The M-mode composition allows a natural mechanical interpretation.

Oscillatory erosion and transport flume with superimposed unidirectional flow

DOEpatents

Jepsen, Richard A.; Roberts, Jesse D.

2004-01-20

A method and apparatus for measuring erosion rates of sediments and at high shear stresses due to complex wave action with, or without, a superimposed unidirectional current. Water is forced in a channel past an exposed sediment core sample, which erodes sediments when a critical shear stress has been exceeded. The height of the core sample is adjusted during testing so that the sediment surface remains level with the bottom of the channel as the sediments erode. Complex wave action is simulated by driving tandom piston/cylinder mechanisms with computer-controlled stepper motors. Unidirectional flow, forced by a head difference between two open tanks attached to each end of the channel, may be superimposed on to the complex wave action. Sediment traps may be used to collect bedload sediments. The total erosion rate equals the change in height of the sediment core sample divided by a fixed period of time.

In vivo wall shear measurements within the developing zebrafish heart.

PubMed

Jamison, R Aidan; Samarage, Chaminda R; Bryson-Richardson, Robert J; Fouras, Andreas

2013-01-01

Physical forces can influence the embryonic development of many tissues. Within the cardiovascular system shear forces resulting from blood flow are known to be one of the regulatory signals that shape the developing heart. A key challenge in investigating the role of shear forces in cardiac development is the ability to obtain shear force measurements in vivo. Utilising the zebrafish model system we have developed a methodology that allows the shear force within the developing embryonic heart to be determined. Accurate wall shear measurement requires two essential pieces of information; high-resolution velocity measurements near the heart wall and the location and orientation of the heart wall itself. We have applied high-speed brightfield imaging to capture time-lapse series of blood flow within the beating heart between 3 and 6 days post-fertilization. Cardiac-phase filtering is applied to these time-lapse images to remove the heart wall and other slow moving structures leaving only the red blood cell movement. Using particle image velocimetry to calculate the velocity of red blood cells in different regions within the heart, and using the signal-to-noise ratio of the cardiac-phase filtered images to determine the boundary of blood flow, and therefore the position of the heart wall, we have been able to generate the necessary information to measure wall shear in vivo. We describe the methodology required to measure shear in vivo and the application of this technique to the developing zebrafish heart. We identify a reduction in shear at the ventricular-bulbar valve between 3 and 6 days post-fertilization and demonstrate that the shear environment of the ventricle during systole is constantly developing towards a more uniform level.

Variables affecting the acceptability of radappertized ground beef products. Effects of food grade phosphates, NaCl, fat level, and grinding methods

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cohen, J.S.; Shults, G.W.; Mason, V.C.

1977-01-01

A series of experiments was conducted to determine the effect of different variables on the quality of an irradiated ground beef product. Factors studied included: different food-grade phosphates; NaCl content; fat content; and size of grind. The influence of these variables on the cooking loss (moisture retention), shear press values and sensory scores was studied. The addition of phosphates and NaCl was desirable in controlling cooking losses. The most effective phosphate was tetrasodium pyrophosphate. The addition of NaCl decreased the shear press force required to penetrate the beef patty, i.e., it tenderized the product. Phosphate addition did not affect themore » shear press force. Increased fat content increased the cooking losses, but did not affect the shear press force. Irradiation with sterilizing doses had a marked effect on decreasing the shear press force.« less

Watching How Molecules Orient in a Surface Forces Apparatus, Using Confocal Raman Spectroscopy

NASA Astrophysics Data System (ADS)

Jiang, Shan; Kim, Minsu; Bae, Sung Chul; Granick, Steve

2006-03-01

Much is known about surface forces, less about where they come from. This laboratory is engaged in direct vibrational spectroscopic measurements of how molecules orient in confined geometries. Regarding force measurements, PDMS (polydimethylsiloxane) was a model system for many years. In this study, we describe direct experiments using a novel version of confocal Raman spectroscopy. This experiment allows direct measurement of how the PDMS molecules orient under confinment as well as under subsequent shear. When the thickness of the fluid film is less than the unperturbed radius of gyration of the polymer, we obtain two novel findings: (a) linewidth analysis of peaks reveals that vibrational relaxation times are perturbed in this confined geometry; (b) orientation of the chain backbone is not everywhere the same within the molecularly-thin film; domains of various orientation are observed instead.

Study on shearing force and impact force of a volcanic mud flow on Mt. Sakurajima

Treesearch

Yoshinobu Taniguchi

1991-01-01

Two kinds of shearing stress meters (type A and type B) were set on the channel bottom in the Arimura River and the Mochiki River on Mt. Sakurajima. Volcanic mud flows take place there about 100 times a year. The results of the surveys demonstrated that the actual shearing force of a volcanic mud flow on Mt. Sakurajima was from 0.46 to 2.50 kgf/cm2...

An observational analysis of a derecho in South China

NASA Astrophysics Data System (ADS)

Xia, Rudi; Wang, Donghai; Sun, Jianhua; Wang, Gaili; Xia, Guancong

2012-12-01

Derechos occur frequently in Europe and the United States, but reports of derechos in China are scarce. In this paper, radar, satellite, and surface observation data are used to analyze a derecho event in South China on 17 April 2011. A derecho-producing mesoscale convective system formed in an environment with medium convective available energy, strong vertical wind shear, and a dry layer in the middle troposphere, and progressed southward in tandem with a front and a surface wind convergence line. The windstorm can be divided into two stages according to differences in the characteristics of the radar echo and the causes of the gale. One stage was a supercell stage, in which the sinking rear inflow of a high-precipitation supercell with a bow-shaped radar echo induced a Fujita F0 class gale. The other stage was a non-supercell stage (the echo was sequentially kidney-shaped, foot-shaped, and an ordinary single cell), in which downbursts induced a gale in Fujita F1 class. This derecho event had many similarities with derechos observed in western countries. For example, the windstorm was perpendicular to the mean flow, the gale was located in the bulging portion of the bow echo, and the derecho moved southward along with the surface front. Some differences were observed as well. The synoptic-scale forcing was weak in the absence of an advancing high-amplitude midlevel trough and an accompanying strong surface cyclone; however, the vertical wind shear was very strong, a characteristic typical of derechos associated with strong synoptic-scale forcing. Extremely high values of convective available potential energy and downdraft convective available potential energy have previously been considered necessary to the formation of weak-forcing archetype and hybrid derechos; however, these values were much less than 2000 J during this derecho event.

Contributions of nanodiamond abrasives and deionized water in magnetorheological finishing of aluminum oxynitriden

NASA Astrophysics Data System (ADS)

Miao, Chunlin; Lambropoulos, John C.; Romanofsky, Henry; Shafrir, Shai N.; Jacobs, Stephen D.

2009-08-01

Magnetorheological finishing (MRF) is a sub-aperture deterministic process for fabricating high-precision optics by removing material and smoothing the surface. The goal of this work is to study the relative contribution of nanodiamonds and water in material removal for MRF of aluminum oxynitride ceramic (ALON) based upon a nonaqueous magnetorheological (MR) fluid. Removal was enhanced by a high carbonyl iron concentration and the addition of nanodiamond abrasives. Small amounts of deionized (DI) water were introduced into the nonaqueous MR fluid to further influence the material removal process. Material removal data were collected with a spot-taking machine. Drag force (Fd) and normal force (Fn) before and after adding nanodiamonds or DI water were measured with a dual load cell. Both drag force and normal force were insensitive to the addition of nanodiamonds but increased with DI water content in the nonaqueous MR fluid. Shear stress (i.e., drag force divided by spot area) was calculated, and examined as a function of nanodiamond concentration and DI water concentration. Volumetric removal rate increased with increasing shear stress, which was shown to be a result of increasing viscosity after adding nanodiamonds and DI water. This work demonstrates that removal rate for a hard ceramic with MRF can be enhanced by adding DI water into a nonaqueous MR fluid.

Estimates of stress drop and crustal tectonic stress from the 27 February 2010 Maule, Chile, earthquake: Implications for fault strength

USGS Publications Warehouse

Luttrell, K.M.; Tong, X.; Sandwell, D.T.; Brooks, B.A.; Bevis, M.G.

2011-01-01

The great 27 February 2010 Mw 8.8 earthquake off the coast of southern Chile ruptured a ???600 km length of subduction zone. In this paper, we make two independent estimates of shear stress in the crust in the region of the Chile earthquake. First, we use a coseismic slip model constrained by geodetic observations from interferometric synthetic aperture radar (InSAR) and GPS to derive a spatially variable estimate of the change in static shear stress along the ruptured fault. Second, we use a static force balance model to constrain the crustal shear stress required to simultaneously support observed fore-arc topography and the stress orientation indicated by the earthquake focal mechanism. This includes the derivation of a semianalytic solution for the stress field exerted by surface and Moho topography loading the crust. We find that the deviatoric stress exerted by topography is minimized in the limit when the crust is considered an incompressible elastic solid, with a Poisson ratio of 0.5, and is independent of Young's modulus. This places a strict lower bound on the critical stress state maintained by the crust supporting plastically deformed accretionary wedge topography. We estimate the coseismic shear stress change from the Maule event ranged from-6 MPa (stress increase) to 17 MPa (stress drop), with a maximum depth-averaged crustal shear-stress drop of 4 MPa. We separately estimate that the plate-driving forces acting in the region, regardless of their exact mechanism, must contribute at least 27 MPa trench-perpendicular compression and 15 MPa trench-parallel compression. This corresponds to a depth-averaged shear stress of at least 7 MPa. The comparable magnitude of these two independent shear stress estimates is consistent with the interpretation that the section of the megathrust fault ruptured in the Maule earthquake is weak, with the seismic cycle relieving much of the total sustained shear stress in the crust. Copyright 2011 by the American Geophysical Union.

Research on the Mechanism of In-Plane Vibration on Friction Reduction

PubMed Central

Wang, Peng; Ni, Hongjian; Wang, Ruihe; Liu, Weili; Lu, Shuangfang

2017-01-01

A modified model for predicting the friction force between drill-string and borehole wall under in-plane vibrations was developed. It was found that the frictional coefficient in sliding direction decreased significantly after applying in-plane vibration on the bottom specimen. The friction reduction is due to the direction change of friction force, elastic deformation of surface asperities and the change of frictional coefficient. Normal load, surface topography, vibration direction, velocity ratio and interfacial shear factor are the main influence factors of friction force in sliding direction. Lower driving force can be realized for a pair of determinate rubbing surfaces under constant normal load by setting the driving direction along the minimum arithmetic average attack angle direction, and applying intense longitudinal vibration on the rubbing pair. The modified model can significantly improve the accuracy in predicting frictional coefficient under vibrating conditions, especially under the condition of lower velocity ratio. The results provide a theoretical gist for friction reduction technology by vibrating drill-string, and provide a reference for determination of frictional coefficient during petroleum drilling process, which has great significance for realizing digitized and intelligent drilling. PMID:28862679

[Studies on the saliva adsorption and the salivary film property on the hydroxyapatite surface].

PubMed

Yao, Jiang-wu; Chen, Guo-yang; Lin, Feng; Lin, Chang-jian; Tao, Tao

2012-07-01

To evaluate the thickness and viscoelasticity of whole saliva (WS), parotid saliva (PS) and submandibular/sublingual gland saliva (SMSLS) film adsorption on the hydroxyapatite (HA) surface. Ultra-thin layer of HA nanocrystals was coated on the dissipation TiO(2) sensor of gold quartz crystal microbalance using electrophoretic deposition technique. The thickness of the HA layer was measured by the ellipsometer, and element analysis was conducted using X-ray photoelectron spectroscopy. Atomic force microscopy and scanning electron microscope were used to observe its morphology. The in-situ adsorption thickness, the shear elastic modulus and the shear viscosity of salivary layers (WS, PS and SMSLS) on HA surfaces were investigated. The statistical data were analysed by an one-way ANOVA analysis followed by a SNK-q test. The results show that the HA layer was a plate-like morphology with 1.53 ± 0.12 in Ca/P molar ratio, (19.1 ± 0.9) nm in the thickness and (6.5 ± 1.6) nm in the roughness. The thickness of salivary film was SMSLS [(21.84 ± 1.25) nm] > WS[(17.91 ± 1.35) nm] > PS [(14.30 ± 1.03 nm) (P < 0.05). The shear elastic modulus of salivary film was PS [(0.61 ± 0.01) MPa] > SMSLS [(0.31 ± 0.09) MPa] and WS [(0.25 ± 0.03) MPa] (P < 0.05). The trend of the shear viscosity was opposite to one of thickness. The characteristics of saliva adsorption on HA surface suggest that the thicker, softer and more hydrated properties for the SMSLS and WS films are likely to afford a stronger lubrication to protect oral surfaces from wear and dehydration. The viscoelasticity of the PS film is probably related to the retention covering the oral cavity.

Protective Performance of Plate-Cell Rubber Tiles against Childhood Head Injury on Playground Surfaces — A Finite Element Analysis

NASA Astrophysics Data System (ADS)

Chang, Li-Tung; Huang, Tsai-Jeon

Rubber tiles are commonly used in playgrounds as protective surfacing to reduce the incidence of head injuries in children caused by falling from equipment. This study developed a rubber tile model consisting of a surface layer of solid and a base layer of plate-cell and used it to investigate head injury protective performance. An explicit finite element method based on the experimental data was used to simulate head impact on the rubber tile. The peak acceleration and head injury criterion (HIC) were employed to assess the shock-absorbing capability of the tile. The results showed that compared to the peak acceleration, use of the HIC index provided a more conservative assessment of the shock absorption ability, and ultimately the protection against head injuries. This study supports the feasibility of using rubber tile with plate-cell construction to improve shock-absorbing capability. The plate-cell structure provided an excellent cushioning effect via a lower axial shear stiffness of the surface layer and lower transverse shearing stiffness of the core. The core's dimensions were an important parameter in determining the shearing stiffness. The analysis suggested that the cushioning effect would significantly reduce the peak force on the head from a fall and delay the occurrence of the peak value during impact, resulting in a marked reduction in the peak acceleration and HIC values of the head. Two plate-cell constructions with honeycomb and box-like cores were proposed and validated in this study. The better protective ability of the honeycomb core was attributed to its lower transverse shearing stiffness.

Shear-induced formation of vesicles in membrane phases: Kinetics and size selection mechanisms, elasticity versus surface tension

NASA Astrophysics Data System (ADS)

Courbin, L.; Panizza, P.

2004-02-01

Multilamellar vesicles can be formed upon shearing lamellar phases (Lα) and phase-separated lamellar-sponge (Lα/L3) mixtures. In the first case, the vesicle volume fraction is always 100% and the vesicle size is monitored by elasticity (“onion textures”). In the second system the vesicle volume fraction can be tuned from 0 to 100% and the mean size results from a balance between capillary and viscous forces (“Taylor droplets”). However, despite these differences, in both systems we show that the formation of vesicles is a strain-controlled process monitored by a universal primary buckling instability of the lamellae.

Amending the W* Velocity Scale for Surface Layer, Entrainment Zone, and Baroclinic Shear in Mixed Forced/Free Turbulent Convection

DTIC Science & Technology

1992-03-30

transitionally turbulent by nature. Thus, we expect Rh to fluctuate about some critical threshold turbulence value, RCh. R,, is much larger than the 1/4...the EZ shear results from turning the wind into the v direction. So for a mature mid-latitude BL with u. 0.4ms 1, f =14S-1, z’ = 103m , e = 300 0C, and...will diminish later as zi becomes large. If we require more accuracy, we can couple eqns. (16, 18, 19, 20, and 26) with 2u. 2 v We v2,& G H’t = We + ( Rh

Guidelines for Finite Element Modeling of Acoustic Radiation Force-Induced Shear Wave Propagation in Tissue-Mimicking Media

PubMed Central

Palmeri, Mark L.; Qiang, Bo; Chen, Shigao; Urban, Matthew W.

2017-01-01

Ultrasound shear wave elastography is emerging as an important imaging modality for evaluating tissue material properties. In its practice, some systematic biases have been associated with ultrasound frequencies, focal depths and configuration, transducer types (linear versus curvilinear), along with displacement estimation and shear wave speed estimation algorithms. Added to that, soft tissues are not purely elastic, so shear waves will travel at different speeds depending on their spectral content, which can be modulated by the acoustic radiation force excitation focusing, duration and the frequency-dependent stiffness of the tissue. To understand how these different acquisition and material property parameters may affect measurements of shear wave velocity, simulations of the propagation of shear waves generated by acoustic radiation force excitations in viscoelastic media are a very important tool. This article serves to provide an in-depth description of how these simulations are performed. The general scheme is broken into three components: (1) simulation of the three-dimensional acoustic radiation force push beam, (2) applying that force distribution to a finite element model, and (3) extraction of the motion data for post-processing. All three components will be described in detail and combined to create a simulation platform that is powerful for developing and testing algorithms for academic and industrial researchers involved in making quantitative shear wave-based measurements of tissue material properties. PMID:28026760

The breakup mechanism of biomolecular and colloidal aggregates in a shear flow

NASA Astrophysics Data System (ADS)

Ó Conchúir, Breanndán; Zaccone, Alessio

2014-03-01

The theory of self-assembly of colloidal particles in shear flow is incomplete. Previous analytical approaches have failed to capture the microscopic interplay between diffusion, shear and intermolecular interactions which controls the aggregates fate in shear. In this work we analytically solved the drift-diffusion equation for the breakup rate of a dimer in flow. Then applying rigidity percolation theory, we found that the lifetime of a generic cluster formed under shear is controlled by the typical lifetime of a single bond in its interior, which in turn depends on the efficiency of the stress transmitted from other bonds in the cluster. We showed that aggregate breakup is a thermally-activated process where the activation energy is controlled by the interplay between intermolecular forces and the shear drift, and where structural parameters determine whether cluster fragmentation or surface erosion prevails. In our latest work, we analyzed floppy modes and nonaffine deformations to derive a lower bound on the fractal dimension df below which aggregates are mechanically unstable, ie. for large aggregates df ~= 2.4. This theoretical framework is in quantitative agreement with experiments and can be used for population balance modeling of colloidal and protein aggregation.

Flexible Micropost Arrays for Shear Stress Measurement

NASA Technical Reports Server (NTRS)

Wohl, Christopher J.; Palmieri, Frank L.; Hopkins, John W.; Jackson, Allen M.; Connell, John W.; Lin, Yi; Cisotto, Alexxandra A.

2015-01-01

Increased fuel costs, heightened environmental protection requirements, and noise abatement continue to place drag reduction at the forefront of aerospace research priorities. Unfortunately, shortfalls still exist in the fundamental understanding of boundary-layer airflow over aerodynamic surfaces, especially regarding drag arising from skin friction. For example, there is insufficient availability of instrumentation to adequately characterize complex flows with strong pressure gradients, heat transfer, wall mass flux, three-dimensionality, separation, shock waves, and transient phenomena. One example is the acoustic liner efficacy on aircraft engine nacelle walls. Active measurement of shear stress in boundary layer airflow would enable a better understanding of how aircraft structure and flight dynamics affect skin friction. Current shear stress measurement techniques suffer from reliability, complexity, and airflow disruption, thereby compromising resultant shear stress data. The state-of-the-art for shear stress sensing uses indirect or direct measurement techniques. Indirect measurements (e.g., hot-wire, heat flux gages, oil interferometry, laser Doppler anemometry, small scale pressure drag surfaces, i.e., fences) require intricate knowledge of the studied flow, restrictive instrument arrangements, large surface areas, flow disruption, or seeding material; with smaller, higher bandwidth probes under development. Direct measurements involve strain displacement of a sensor element and require no prior knowledge of the flow. Unfortunately, conventional "floating" recessed components for direct measurements are mm to cm in size. Whispering gallery mode devices and Fiber Bragg Gratings are examples of recent additions to this type of sensor with much smaller (?m) sensor components. Direct detection techniques are often single point measurements and difficult to calibrate and implement in wind tunnel experiments. In addition, the wiring, packaging, and installation of delicate micro-electromechanical devices impede the use of most direct shear sensors. Similarly, the cavity required for sensing element displacement is sensitive to particulate obstruction. This work was focused on developing a shear stress sensor for use in subsonic wind tunnel test facilities applicable to an array of test configurations. The non-displacement shear sensors described here have minimal packaging requirements resulting in minimal or no disturbance of boundary layer flow. Compared to previous concepts, device installation could be simple with reduced cost and down-time. The novelty lies in the creation of low profile (nanoscale to 100 µm) micropost arrays that stay within the viscous sub-layer of the airflow. Aerodynamic forces, which are related to the surface shear stress, cause post deflection and optical property changes. Ultimately, a reliable, accurate shear stress sensor that does not disrupt the airflow has the potential to provide high value data for flow physics researchers, aerodynamicists, and aircraft manufacturers leading to greater flight efficiency arising from more in-depth knowledge on how aircraft design impacts near surface properties.

High friction on ice provided by elastomeric fiber composites with textured surfaces

NASA Astrophysics Data System (ADS)

Rizvi, R.; Naguib, H.; Fernie, G.; Dutta, T.

2015-03-01

Two main applications requiring high friction on ice are automobile tires and footwear. The main motivation behind the use of soft rubbers in these applications is the relatively high friction force generated between a smooth rubber contacting smooth ice. Unfortunately, the friction force between rubber and ice is very low at temperatures near the melting point of ice and as a result we still experience automobile accidents and pedestrian slips and falls in the winter. Here, we report on a class of compliant fiber-composite materials with textured surfaces that provide outstanding coefficients of friction on wet ice. The fibrous composites consist of a hard glass-fiber phase reinforcing a compliant thermoplastic polyurethane matrix. The glass-fiber phase is textured such that it is aligned transversally and protruding out of the elastomer surface. Our analysis indicates that the exposed fiber phase exhibits a "micro-cleat" effect, allowing for it to fracture the ice and increase the interfacial contact area thereby requiring a high force to shear the interface.

Kinetics of the head-neck complex in low-speed rear impact.

PubMed

Stemper, Brian D; Yoganandan, Naryan; Pintar, Frank A

2003-01-01

A comprehensive characterization of the biomechanics of the cervical spine in rear impact will lead to an understanding of the mechanisms of whiplash injury. Cervical kinematics have been experimentally described using human volunteers, full-body cadaver specimens, and isolated and intact head-neck specimens. However, forces and moments at the cervico-thoracic junction have not been clearly delineated. An experimental investigation was performed using ten intact head-neck complexes to delineate the loading at the base of the cervical spine and angular acceleration of the head in whiplash. A pendulum-minisled apparatus was used to simulate whiplash acceleration of the thorax at four impact severities. Lower neck loads were measured using a six-axis load cell attached between the minisled and head-neck specimens, and head angular motion was measured with an angular rate sensor attached to the lateral side of the head. Shear and axial force, extension moment, and head angular acceleration increased with impact severity. Shear force was significantly larger than axial force (p < 0.0001). Shear force reached its maximum value at 46 msec. Maximum extension moment occurred between 7 and 22 msec after maximum shear force. Maximum angular acceleration of the head occurred 2 to 18 msec later. Maximum axial force occurred last (106 msec). All four kinetic components reached maximum values during cervical S-curvature, with maximum shear force and extension moment occurring before the attainment of maximum S-curvature. Results of the present investigation indicate that shear force and extension moment at the cervico-thoracic junction drive the non-physiologic cervical S-curvature responsible for whiplash injury and underscore the importance of understanding cervical kinematics and the underlying kinetics.

Dynamics of ultrasonic additive manufacturing.

PubMed

Hehr, Adam; Dapino, Marcelo J

2017-01-01

Ultrasonic additive manufacturing (UAM) is a solid-state technology for joining similar and dissimilar metal foils near room temperature by scrubbing them together with ultrasonic vibrations under pressure. Structural dynamics of the welding assembly and work piece influence how energy is transferred during the process and ultimately, part quality. To understand the effect of structural dynamics during UAM, a linear time-invariant model is proposed to relate the inputs of shear force and electric current to resultant welder velocity and voltage. Measured frequency response and operating performance of the welder under no load is used to identify model parameters. Using this model and in-situ measurements, shear force and welder efficiency are estimated to be near 2000N and 80% when welding Al 6061-H18 weld foil, respectively. Shear force and welder efficiency have never been estimated before in UAM. The influence of processing conditions, i.e., welder amplitude, normal force, and weld speed, on shear force and welder efficiency are investigated. Welder velocity was found to strongly influence the shear force magnitude and efficiency while normal force and weld speed showed little to no influence. The proposed model is used to describe high frequency harmonic content in the velocity response of the welder during welding operations and coupling of the UAM build with the welder. Copyright © 2016 Elsevier B.V. All rights reserved.

Measurement of the Surface Dilatational Viscosity of an Insoluble Surfactant Monolayer at the Air/Water Interface Using a Pendant Drop Apparatus

NASA Technical Reports Server (NTRS)

Lorenzo, Jose; Couzis, Alex; Maldarelli, Charles; Singh, Bhim S. (Technical Monitor)

2000-01-01

When a fluid interface with surfactants is at rest, the interfacial stress is isotropic (as given by the equilibrium interfacial tension), and is described by the equation of state which relates the surface tension to the surfactant surface concentration. When surfactants are subjected to shear and dilatational flows, flow induced interaction of the surfactants; can create interfacial stresses apart from the equilibrium surface tension. The simplest relationship between surface strain rate and surface stress is the Boussinesq-Scriven constitutive equation completely characterized by three coefficients: equilibrium interfacial tension, surface shear viscosity, and surface dilatational viscosity Equilibrium interfacial tension and surface shear viscosity measurements are very well established. On the other hand, surface dilatational viscosity measurements are difficult because a flow which change the surface area also changes the surfactant surface concentration creating changes in the equilibrium interfacial tension that must be also taken into account. Surface dilatational viscosity measurements of existing techniques differ by five orders of magnitude and use spatially damped surface waves and rapidly expanding bubbles. In this presentation we introduce a new technique for measuring the surface dilatational viscosity by contracting an aqueous pendant drop attached to a needle tip and having and insoluble surfactant monolayer at the air-water interface. The isotropic total tension on the surface consists of the equilibrium surface tension and the tension due to the dilation. Compression rates are undertaken slow enough so that bulk hydrodynamic stresses are small compared to the surface tension force. Under these conditions we show that the total tension is uniform along the surface and that the Young-Laplace equation governs the drop shape with the equilibrium surface tension replaced by the constant surface isotropic stress. We illustrate this technique using DPPC as the insoluble surfacant monolayer and measured for it a surface dilatational viscosity in the LE phase that is 20 surface poise.

Non-canonical Wnt signalling modulates the endothelial shear stress flow sensor in vascular remodelling

PubMed Central

Franco, Claudio A; Jones, Martin L; Bernabeu, Miguel O; Vion, Anne-Clemence; Barbacena, Pedro; Fan, Jieqing; Mathivet, Thomas; Fonseca, Catarina G; Ragab, Anan; Yamaguchi, Terry P; Coveney, Peter V; Lang, Richard A; Gerhardt, Holger

2016-01-01

Endothelial cells respond to molecular and physical forces in development and vascular homeostasis. Deregulation of endothelial responses to flow-induced shear is believed to contribute to many aspects of cardiovascular diseases including atherosclerosis. However, how molecular signals and shear-mediated physical forces integrate to regulate vascular patterning is poorly understood. Here we show that endothelial non-canonical Wnt signalling regulates endothelial sensitivity to shear forces. Loss of Wnt5a/Wnt11 renders endothelial cells more sensitive to shear, resulting in axial polarization and migration against flow at lower shear levels. Integration of flow modelling and polarity analysis in entire vascular networks demonstrates that polarization against flow is achieved differentially in artery, vein, capillaries and the primitive sprouting front. Collectively our data suggest that non-canonical Wnt signalling stabilizes forming vascular networks by reducing endothelial shear sensitivity, thus keeping vessels open under low flow conditions that prevail in the primitive plexus. DOI: http://dx.doi.org/10.7554/eLife.07727.001 PMID:26845523

Shear thinning in non-Brownian suspensions.

PubMed

Chatté, Guillaume; Comtet, Jean; Niguès, Antoine; Bocquet, Lydéric; Siria, Alessandro; Ducouret, Guylaine; Lequeux, François; Lenoir, Nicolas; Ovarlez, Guillaume; Colin, Annie

2018-02-14

We study the flow of suspensions of non-Brownian particles dispersed into a Newtonian solvent. Combining capillary rheometry and conventional rheometry, we evidence a succession of two shear thinning regimes separated by a shear thickening one. Through X-ray radiography measurements, we show that during each of those regimes, the flow remains homogeneous and does not involve particle migration. Using a quartz-tuning fork based atomic force microscope, we measure the repulsive force profile and the microscopic friction coefficient μ between two particles immersed into the solvent, as a function of normal load. Coupling measurements from those three techniques, we propose that (1) the first shear-thinning regime at low shear rates occurs for a lubricated rheology and can be interpreted as a decrease of the effective volume fraction under increasing particle pressures, due to short-ranged repulsive forces and (2) the second shear thinning regime after the shear-thickening transition occurs for a frictional rheology and can be interpreted as stemming from a decrease of the microscopic friction coefficient at large normal load.

Microscopic Origins of Shear Jamming for 2D Frictional Grains

NASA Astrophysics Data System (ADS)

Wang, Dong; Ren, Jie; Dijksman, Joshua A.; Zheng, Hu; Behringer, Robert P.

2018-05-01

Shear jamming (SJ) occurs for frictional granular materials with packing fractions ϕ in ϕS<ϕ <ϕJ0, when the material is subject to shear strain γ starting from a force-free state. Here, ϕJμ is the isotropic jamming point for particles with a friction coefficient μ . SJ states have mechanically stable anisotropic force networks, e.g., force chains. Here, we investigate the origins of SJ by considering small-scale structures—trimers and branches—whose response to shear leads to SJ. Trimers are any three grains where the two outer grains contact a center one. Branches occur where three or more quasilinear force chain segments intersect. Certain trimers respond to shear by compressing and bending; bending is a nonlinear symmetry-breaking process that can push particles in the dilation direction faster than the affine dilation. We identify these structures in physical experiments on systems of two-dimensional frictional discs, and verify their role in SJ. Trimer bending and branch creation both increase Z above Ziso≃3 needed for jamming 2D frictional grains, and grow the strong force network, leading to SJ.

Direct manipulation of metallic nanosheets by shear force microscopy.

PubMed

Bi, Z; Cai, W; Wang, Y; Shang, G

2018-05-15

Micro/nanomanipulation is a rapidly growing technology and holds promising applications in various fields, including photonic/electronic devices, chemical/biosensors etc. In this work, we present that shear force microscopy (ShFM) can be exploited to manipulate metallic nanosheets besides imaging. The manipulation is realized via controlling the shear force sensor probe position and shear force magnitude based on our homemade ShFM system under an optical microscopy for in situ observation. The main feature of the ShFM system is usage of a piezoelectric bimorph sensor, which has the ability of self-excitation and detection. Moreover, the shear force magnitude as a function of the spring constant of the sensor and setpoint is obtained, which indicates that operation modes can be switched between imaging and manipulation through designing the spring constant before experiment and changing the setpoint during manipulation process, respectively. We believe that this alternative manipulation technique could be used to assemble other nanostructures with different shapes, sizes and compositions for new properties and wider applications. © 2018 The Authors Journal of Microscopy © 2018 Royal Microscopical Society.

Experimental investigation on the weld pool formation process in plasma keyhole arc welding

NASA Astrophysics Data System (ADS)

Van Anh, Nguyen; Tashiro, Shinichi; Van Hanh, Bui; Tanaka, Manabu

2018-01-01

This paper seeks to clarify the weld pool formation process in plasma keyhole arc welding (PKAW). We adopted, for the first time, the measurement of the 3D convection inside the weld pool in PKAW by stereo synchronous imaging of tungsten tracer particles using two sets of x-ray transmission systems. The 2D convection on the weld pool surface was also measured using zirconia tracer particles. Through these measurements, the convection in a wide range of weld pools from the vicinity of the keyhole to the rear region was successfully visualized. In order to discuss the heat transport process in a weld pool, the 2D temperature distribution on the weld pool surface was also measured by two-color pyrometry. The results of the comprehensive experimental measurement indicate that the shear force due to plasma flow is found to be the dominant driving force in the weld pool formation process in PKAW. Thus, heat transport in a weld pool is considered to be governed by two large convective patterns near the keyhole: (1) eddy pairs on the surface (perpendicular to the torch axis), and (2) eddy pairs on the bulk of the weld pool (on the plane of the torch). They are formed with an equal velocity of approximately 0.35 m s-1 and are mainly driven by shear force. Furthermore, the flow velocity of the weld pool convection becomes considerably higher than that of other welding processes, such as TIG welding and GMA welding, due to larger plasma flow velocity.

GPU-based Green's function simulations of shear waves generated by an applied acoustic radiation force in elastic and viscoelastic models.

PubMed

Yang, Yiqun; Urban, Matthew W; McGough, Robert J

2018-05-15

Shear wave calculations induced by an acoustic radiation force are very time-consuming on desktop computers, and high-performance graphics processing units (GPUs) achieve dramatic reductions in the computation time for these simulations. The acoustic radiation force is calculated using the fast near field method and the angular spectrum approach, and then the shear waves are calculated in parallel with Green's functions on a GPU. This combination enables rapid evaluation of shear waves for push beams with different spatial samplings and for apertures with different f/#. Relative to shear wave simulations that evaluate the same algorithm on an Intel i7 desktop computer, a high performance nVidia GPU reduces the time required for these calculations by a factor of 45 and 700 when applied to elastic and viscoelastic shear wave simulation models, respectively. These GPU-accelerated simulations also compared to measurements in different viscoelastic phantoms, and the results are similar. For parametric evaluations and for comparisons with measured shear wave data, shear wave simulations with the Green's function approach are ideally suited for high-performance GPUs.

Constant-Distance Mode Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Biological Samples with Complex Topography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nguyen, Son N.; Liyu, Andrey V.; Chu, Rosalie K.

A new approach for constant distance mode mass spectrometry imaging of biological samples using nanospray desorption electrospray ionization (nano-DESI MSI) was developed by integrating a shear-force probe with nano-DESI probe. The technical concept and basic instrumental setup as well as general operation of the system are described. Mechanical dampening of resonant oscillations due to the presence of shear forces between the probe and the sample surface enables constant-distance imaging mode via a computer controlled closed feedback loop. The capability of simultaneous chemical and topographic imaging of complex biological samples is demonstrated using living Bacillus Subtilis ATCC 49760 colonies on agarmore » plates. The constant-distance mode nano-DESI MSI enabled imaging of many metabolites including non-ribosomal peptides (surfactin, plipastatin and iturin) and iron-bound heme on the surface of living bacterial colonies ranging in diameter from 10 mm to 13 mm with height variations of up to 0.8 mm above the agar plate. Co-registration of ion images to topographic images provided higher-contrast images. Constant-mode nano-DESI MSI is ideally suited for imaging biological samples of complex topography in their native state.« less

Numerical Study of High-Speed Droplet Impact on Surfaces and its Physical Cleaning Effects

NASA Astrophysics Data System (ADS)

Kondo, Tomoki; Ando, Keita

2015-11-01

Spurred by the demand for cleaning techniques of low environmental impact, one favors physical cleaning that does not rely on any chemicals. One of the promising candidates is based on water jets that often involve fission into droplet fragments and collide with target surfaces to which contaminant particles (often micron-sized or even smaller) stick. Hydrodynamic force (e.g., shearing and lifting) arising from the droplet impact will play a role to remove the particles, but its detailed mechanism is still unknown. To explore the role of high-speed droplet impact in physical cleaning, we solve compressible Navier-Stokes equations with a finite volume method that is designed to capture both shocks and material interfaces in accurate and robust manners. Water hammer and shear flow accompanied by high-speed droplet impact at a rigid wall is simulated to evaluate lifting force and rotating torque, which are relevant to the application of particle removal. For the simulation, we use the numerical code recently developed by Computational Flow Group lead by Tim Colonius at Caltech. The first author thanks Jomela Meng for her help in handling the code during his stay at Caltech.

Flagellated bacteria trace out a parabolic arc under low shear condition

NASA Astrophysics Data System (ADS)

Ahn, Yongtae; Hashmi, Sara; Walker, Sharon; Hill, Jane

2010-03-01

The measurement and prediction of bacterial transport of bacteria in aquatic systems is of fundamental importance to a variety of fields such as groundwater bioremediation ascending urinary tract infection. The motility of pathogenic bacteria is, however, often missing when considering pathogen translocation prediction. Previously, we reported that flagellated E. coli can translate upstream under low shear flow conditions (Hill et al., 2007). The upstream swimming of flagellated microorganisms depends on hydrodynamic interaction between cell body and surrounding fluid flow. In this study, we use a breathable microfluidic device to image swimming E. coli and P. aeruginosa at a glass surface under low shear flow condition. We find the dominant experimental variables that lead to upstream swimming are: fluid shear, bacterium velocity, and bacterium length. We will present data showing that the sum of forces and torques acting on a bacterium lead to them tracing out a parabolic arc as they turn into the flow to swim upstream.

Influence of shear forces on the aggregation and sedimentation behavior of cerium dioxide (CeO2) nanoparticles under different hydrochemical conditions

NASA Astrophysics Data System (ADS)

Lv, Bowen; Wang, Chao; Hou, Jun; Wang, Peifang; Miao, Lingzhan; Li, Yi; Ao, Yanhui; Yang, Yangyang; You, Guoxiang; Xu, Yi

2016-07-01

This study contributed to a better understanding of the behavior of nanoparticles (NPs) in dynamic water. First, the aggregation behavior of CeO2 NPs at different pH values in various salt solutions was examined to determine the appropriate hydrochemical conditions for hydrodynamics study. Second, the aggregation behavior of CeO2 NPs under different shear forces was investigated at pH 4 and ionic strength 0 in various salt solutions to find out whether shear forces could influence the stability of the nanoparticles and if yes, how. Also, five-stage sedimentation tests were conducted to understand the influence of shear stress on the vertical distribution of CeO2 NPs in natural waters. The aggregation test showed that the shear force could increase the collision efficiency between NPs during aggregation and cause a relatively large mass of NPs to remain in suspension. Consequently, the nanoparticles had a greater possibility of continued aggregation. The sedimentation test under static conditions indicated that a large mass of NPs (>1000 nm) sink to the bottom layer, leaving only small aggregates dispersed in the upper or middle layer of the solution. However, later sedimentation studies under stirring conditions demonstrated that shear forces can disrupt this stratification phenomenon. These results suggest that shear forces can influence the spatial distribution of NPs in natural waters, which might lead to different toxicities of CeO2 NPs to aquatic organisms distributed in the different water layers. This study contributes to a better understanding of nanomaterial toxicology and provides a way for further research.

Investigation of viral vectors using atomic force microscopy and microfluidic devices

NASA Astrophysics Data System (ADS)

Negishi, Atsuko

Researchers are modifying viruses into gene delivery vehicles in hope to cure diseases such as muscular dystrophy, hemophilia and cancer. Significant progress has been made toward this end, but further development and success of viral vectors depend on a deeper understanding of viral structure and physiology. Recent advances in microscopy have allowed new approaches to studying viruses that complement existing methodologies. Presented in this dissertation are novel viral studies using the atomic force microscope (AFM), a microscope that provides topographic information at the nanometer scale. As well microfluidic channels were used to study the effect of fluid flow properties on infection. A number of viruses are currently under study as potential vectors. We focus our studies on the adenovirus (Ad) and the adeno-associated virus (AAV) which have numerous attractive properties as vectors. The AFM is used to probe first, the structural aspects of the Ad and second, the virus-receptor interactions between AAV and its cell surface receptor, heparan sulfate proteoglycan (HSPG). The AFM was capable of imaging the capsid facets of intact Ad and DNA strands released from disrupted Ad capsids. In addition, we found that the stability of the capsid depended on the surface chemistry. An AFM-based binding assay was developed to study the binding between AAV and HSPG. The advantage of using the AFM for this purpose is its ability to simultaneously provide structural and quantitative information at the single molecule level. We measured a binding constant of 3.4 +/- 0.3 nM which is consistent with published reports. Microfluidic devices were used to study the dependence of fluid flow on infection. Cells were cultured in microfluidic channels and exposed to AAV vectors at various shear stresses. We found that a lower percentage of the cells were infected at higher shear stress. We also found that fluid forces can indirectly play a role in viral infection by influencing the cell state. A significantly lower percentage of cells that were treated with shear stress prior to vector exposure were infected compared to cells which were not exposed to shear stress.

Thermal cycling effects on adhesion of resin-bovine enamel junction among different composite resins.

PubMed

Chen, Wen-Cheng; Ko, Chia-Ling; Wu, Hui-Yu; Lai, Pei-Ling; Shih, Chi-Jen

2014-10-01

Thermal cycling is used to mimic the changes in oral cavity temperature experienced by composite resins when used clinically. The purpose of this study is to assess the thermal cycling effects of in-house produced composite resin on bonding strength. The dicalcium phosphate anhydrous filler surfaces are modified using nanocrystals and silanization (w/NP/Si). The resin is compared with commercially available composite resins Filtek Z250, Z350, and glass ionomer restorative material GIC Fuji-II LC (control). Different composite resins were filled into the dental enamel of bovine teeth. The bond force and resin-enamel junction graphical structures of the samples were determined after thermal cycling between 5 and 55°C in deionized water for 600 cycles. After thermal cycling, the w/NP/Si 30wt%, 50wt% and Filtek Z250, Z350 groups showed higher shear forces than glass ionomer GIC, and w/NP/Si 50wt% had the highest shear force. Through SEM observations, more of the fillings with w/NP/Si 30wt% and w/NP/Si 50wt% groups flowed into the enamel tubule, forming closed tubules with the composite resins. The push-out force is proportional to the resin flow depth and uniformity. The push-out tubule pore and resin shear pattern is the most uniform and consistent in the w/NP/Si 50wt% group. Accordingly, this developed composite resin maintains great mechanical properties after thermal cycling. Thus, it has the potential to be used in a clinical setting when restoring non-carious cervical lesions. Copyright © 2014 Elsevier Ltd. All rights reserved.

Role of Wall Shear Stress in Cryptosporidium parvum Oocyst Attachment to Environmental Biofilms.

PubMed

Luo, Xia; Jedlicka, Sabrina S; Jellison, Kristen L

2017-12-15

This study investigated Cryptosporidium parvum oocyst deposition onto biofilms as a function of shear stress under laminar or turbulent flow. Annular rotating bioreactors were used to grow stabilized stream biofilms at shear stresses ranging from 0.038 to 0.46 Pa. These steady-state biofilms were then used to assess the impact of hydrodynamic conditions on C. parvum oocyst attachment. C. parvum deposition onto biofilms followed a pseudo-second-order model under both laminar (after a lag phase) and turbulent flows. The total number of oocysts attached to the biofilm at steady state decreased as the hydrodynamic wall shear stress increased. The oocyst deposition rate constant increased with shear stress but decreased at high shear, suggesting that increasing wall shear stress results in faster attachment of Cryptosporidium due to higher mass transport until the shear forces exceed a critical limit that prevents oocyst attachment. These data show that oocyst attachment in the short and long term are impacted differently by shear: higher shear (to a certain limit) may be associated with faster initial oocyst attachment, but lower shear is associated with greater numbers of oocysts attached at equilibrium. IMPORTANCE This research provides experimental evidence to demonstrate that shear stress plays a critical role in protozoan-pathogen transport and deposition in environmental waters. The data presented in this work expand scientific understanding of Cryptosporidium attachment and fate, which will further influence the development of timely and accurate sampling strategies, as well as advanced water treatment technologies, to target protozoan pathogens in surface waters that serve as municipal drinking water sources. Copyright © 2017 American Society for Microbiology.

Modélisation morphodynamique cross-shore d'un estran vaseux

NASA Astrophysics Data System (ADS)

Waeles, Benoı̂t; Le Hir, Pierre; Silva Jacinto, Ricardo

2004-08-01

Numerical experiments were performed to simulate the profile evolution of an intertidal mudflat with a 1D cross-shore morphodynamical model. First, the hydrodynamical forcing is a cross-shore tidal current due to semi-diurnal variations of the free surface elevation at the open boundary. Further, considering the conservation of the action density of surface gravity waves, a wave height (and resulting bottom shear stress) calculation is added to the morphodynamical model. Results of the numerical experiments show that the shape of the profile reaches equilibrium. The mudflat progrades continually when the forcing is tide only, whereas it can be steady under the simultaneous action of tide and waves. To cite this article: B. Waeles et al., C. R. Geoscience 336 (2004).

A flexible micromachined optical sensor for simultaneous measurement of pressure and shear force distribution on foot

NASA Astrophysics Data System (ADS)

Wang, Wei-Chih; Panergo, Reynold R.; Galvanin, Christopher M.; Ledoux, William; Sangeorzan, Bruce; Reinhall, Per G.

2003-07-01

Lower limb complications associated with diabetes include the development of plantar ulcers that can lead to infection and subsequent amputation. While it is known from force plate analyses that there are medial/lateral and anterior/posterior shear components of the ground reaction force, there is little known about the actual distribution of this force during daily activities, nor about the role that shear plays in causing plantar ulceration. Furthermore, one critical reason why these data have not been obtained previously is the lack of a validated, widely used, commercially available shear sensor, in part because of the various technical issues associated with shear measurement. Here we have developed novel means of tranducing plantar shear and pressure stress via a new microfabricated optical system. The pressure/shear sensor consists of an array of optical waveguides lying in perpendicular rows and columns separated by elastomeric pads. A map of pressure and shear stress is constructed based on observed macro bending through the intensity attenuation from the physical deformation of two adjacent perpendicular optical waveguides. The uniqueness of the sensor is in its batch fabrication process, which involves injection molding and embossing techniques with Polydimethylsiloxane (PDMS) as the optical medium. Here we present the preliminary results of the prototype. The sensor has been shown to have low noise and responds linearly to applied loads. The smallest detectable force on each sensor element based on the current setup is ~0.1 N. The smallest area we have resolved in our mesh sensor is currently 950x950μm2

Ultrasound shear wave simulation based on nonlinear wave propagation and Wigner-Ville Distribution analysis

NASA Astrophysics Data System (ADS)

Bidari, Pooya Sobhe; Alirezaie, Javad; Tavakkoli, Jahan

2017-03-01

This paper presents a method for modeling and simulation of shear wave generation from a nonlinear Acoustic Radiation Force Impulse (ARFI) that is considered as a distributed force applied at the focal region of a HIFU transducer radiating in nonlinear regime. The shear wave propagation is simulated by solving the Navier's equation from the distributed nonlinear ARFI as the source of the shear wave. Then, the Wigner-Ville Distribution (WVD) as a time-frequency analysis method is used to detect the shear wave at different local points in the region of interest. The WVD results in an estimation of the shear wave time of arrival, its mean frequency and local attenuation which can be utilized to estimate medium's shear modulus and shear viscosity using the Voigt model.

Colloidal Dispersions in Polymeric Media: Interparticle Forces, Microstructure and Rheology

NASA Astrophysics Data System (ADS)

Ndong, Rose Seynabou

To enhance properties of the ultimate materials, melt processed polymers are commonly filled with colloidal particles, such as inorganic oxides. Dispersing such particles in a melt is generally difficult due to the strong van der Waals attractions. These attractive forces can be modulated through surface modifications such as polymer adsorption and grafting. Indeed, the relative viscosity of 430 nm Al2O3particles stabilized by end-tethered poly(dimethylsiloxane) (PDMS) in PDMS melts decreases with increasing graft density and molecular weight as expected, but also with increasing molecular weight of the melt, contrary to well established theories. The relative steady shear viscosity exhibits neither a low shear limit nor a yield stress, but follows a power law characterized by relative high shear viscosity (eta infinity/mu) and a structural relaxation time (tau). The measured structural time can be correlated reasonably well with a characteristic relaxation time, tauo, estimated by equating the viscous resistance with the maximum attractive force. We further explored the significance of this power law with TiO2 nanoparticles in PDMS melts with a reduction in size and an increase in Hamaker constant. Bare, octadecyl-coated, and 9k-PDMS grafted TiO2 particles dispersed in neat and binary PDMS melts revealed behavior similar to that of the large alumina particles, as the increased strength of van der Waals forces offset the reduction in size. To complete the study ZrO2 nanoparticles were dispersed in solution of associative polymers and characterized by small amplitude oscillatory shear. The data exhibits two relaxation modes: Maxwellian behavior at high frequency imparted by the associating polymers and a power law spectrum at low frequency from the particles. The timescales and volume fraction dependence reflect the attractions between particles with adsorbed polymer layers dispersed in a percolated network of associative polymers. Together these studies demonstrate the range and origin of the rheology possible with particles dispersed in polymeric media.

Room temperature shear properties of the strain isolator pad for the shuttle thermal protection system

NASA Technical Reports Server (NTRS)

Sawyer, J. W.; Waters, W. A., Jr.

1981-01-01

Tests were conducted at room temperature to determine the shear properties of the strain isolator pad (SIP) material used in the thermal protection system of the space shuttle. Tests were conducted on both the .23 cm and .41 cm thick SIP material in the virgin state and after fifty fully reversed shear cycles. The shear stress displacement relationships are highly nonlinear, exhibit large hysteresis effects, are dependent on material orientation, and have a large low modulus region near the zero stress level where small changes in stress can result in large displacements. The values at the higher stress levels generally increase with normal and shear force load conditioning. Normal forces applied during the shear tests reduces the low modulus region for the material. Shear test techniques which restrict the normal movement of the material give erroneous stress displacement results. However, small normal forces do not significantly effect the shear modulus for a given shear stress. Poisson's ratio values for the material are within the range of values for many common materials. The values are not constant but vary as a function of the stress level and the previous stress history of the material. Ultimate shear strengths of the .23 cm thick SIP are significantly higher than those obtained for the .41 cm thick SIP.

The Role of Grain Dynamics in the Onset of Sediment Transport

NASA Astrophysics Data System (ADS)

Clark, A., IV; Shattuck, M. D.; Ouellette, N. T.; O'Hern, C.

2016-12-01

Despite decades of research, the grain-scale mechanisms that control the onset of sediment transport are still not well understood. A large collection of data, known as the Shields curve, shows that Θ c, which is the minimum dimensionless shear stress at the bed for grains to move, is primarily a function of the shear Reynolds number Re*. To understand this collapse, it is typically assumed that the onset of grain motion is determined by the conditions at which fluid forces violate static equilibrium for surface grains. Re* compares the grain size to the size of the viscous sublayer in the fluid flow, so the relevant fluid lift and drag forces vary with Re*. A complimentary approach, which remains relatively unexplored, is to ask instead when mobilized grains can stop. In this case, Re* is the ratio of two important time scales related to grain motion: (1) the time for a grain to equilibrate to the fluid flow and (2) the time for the shear stress to accelerate a grain over the characteristic bed roughness. Thus, Re* controls whether grains are accelerated significantly between collisions with the bed. To test how this effect relates to the Shields curve, we perform simulations of granular beds sheared by a model fluid flow, where Re* is varied only through the fluid-grain coupling, which alters the grain dynamics. We find good qualitative agreement with the Shields curve, and the quantitative discrepancies are consistent with lift forces calculations at varying Re*. Our results suggest that the onset of sediment transport may be better described as when mobile grains are able to stop, which varies significantly with Re*, and theoretical descriptions that account for this effect may be more successful than those that consider only static equilibrium.

Traction patterns of tumor cells.

PubMed

Ambrosi, D; Duperray, A; Peschetola, V; Verdier, C

2009-01-01

The traction exerted by a cell on a planar deformable substrate can be indirectly obtained on the basis of the displacement field of the underlying layer. The usual methodology used to address this inverse problem is based on the exploitation of the Green tensor of the linear elasticity problem in a half space (Boussinesq problem), coupled with a minimization algorithm under force penalization. A possible alternative strategy is to exploit an adjoint equation, obtained on the basis of a suitable minimization requirement. The resulting system of coupled elliptic partial differential equations is applied here to determine the force field per unit surface generated by T24 tumor cells on a polyacrylamide substrate. The shear stress obtained by numerical integration provides quantitative insight of the traction field and is a promising tool to investigate the spatial pattern of force per unit surface generated in cell motion, particularly in the case of such cancer cells.

Transient Reactivation of a Deep-Seated Landslide by Undrained Loading Captured With Repeat Airborne and Terrestrial Lidar

NASA Astrophysics Data System (ADS)

Booth, Adam M.; McCarley, Justin; Hinkle, Jason; Shaw, Susan; Ampuero, Jean-Paul; Lamb, Michael P.

2018-05-01

Landslides reactivate due to external environmental forcing or internal mass redistribution, but the process is rarely documented quantitatively. We capture the three-dimensional, 1-m resolution surface deformation field of a transiently reactivated landslide with image correlation of repeat airborne lidar. Undrained loading by two debris flows in the landslide's head, rather than external forcing, triggered reactivation. After that loading, the lower 2 km of the landslide advanced by up to 14 m in 2 years before completely stopping. The displacement field over those 2 years implies that the slip surface gained 1 kPa of shear strength, which was likely accomplished by a negative dilatancy-pore pressure feedback as material deformed around basal roughness elements. Thus, landslide motion can be decoupled from external environmental forcing in cases, motivating the need to better understand internal perturbations to the stress field to predict hazards and sediment fluxes as landscapes evolve.

The effects of silver coating on friction coefficient and shear bond strength of steel orthodontic brackets.

PubMed

Arash, Valiollah; Anoush, Keivan; Rabiee, Sayed Mahmood; Rahmatei, Manuchehr; Tavanafar, Saeid

2015-01-01

Aims of the present study was to measure frictional resistance between silver coated brackets and different types of arch wires, and shear bond strength of these brackets to the tooth. In an experimental clinical research 28 orthodontic brackets (standard, 22 slots) were coated with silver ions using electroplate method. Six brackets (coated: 3, uncoated: 3) were evaluated with Scanning Electron Microscopy and Atomic Force Microscopy. The amount of friction in 15 coated brackets was measured with three different kinds of arch wires (0.019 × 0.025-in stainless steel [SS], 0.018-in stainless steel [SS], 0.018-in Nickel-Titanium [Ni-Ti]) and compared with 15 uncoated steel brackets. In addition, shear bond strength values were compared between 10 brackets with silver coating and 10 regular brackets. Universal testing machine was used to measure shear bond strength and the amount of friction between the wires and brackets. SPSS 18 was used for data analysis with t-test. SEM and AFM results showed deposition of a uniform layer of silver, measuring 8-10 μm in thickness on bracket surfaces. Silver coating led to higher frictional forces in all the three types of arch wires, which was statistically significant in 0.019 × 0.025-in SS and 0.018-in Ni-Ti, but it did not change the shear bond strength significantly. Silver coating with electroplating method did not affect the bond strength of the bracket to enamel; in addition, it was not an effective method for decreasing friction in sliding mechanics. © Wiley Periodicals, Inc.

Two-dimensional simulation of red blood cell motion near a wall under a lateral force

NASA Astrophysics Data System (ADS)

Hariprasad, Daniel S.; Secomb, Timothy W.

2014-11-01

The motion of a red blood cell suspended in a linear shear flow adjacent to a fixed boundary subject to an applied lateral force directed toward the boundary is simulated. A two-dimensional model is used that represents the viscous and elastic properties of normal red blood cells. Shear rates in the range of 100 to 600 s-1 are considered, and the suspending medium viscosity is 1 cP. In the absence of a lateral force, the cell executes a tumbling motion. With increasing lateral force, a transition from tumbling to tank-treading is predicted. The minimum force required to ensure tank-treading increases nonlinearly with the shear rate. Transient swinging motions occur when the force is slightly larger than the transition value. The applied lateral force is balanced by a hydrodynamic lift force resulting from the positive orientation of the long axis of the cell with respect to the wall. In the case of cyclic tumbling motions, the orientation angle takes positive values through most of the cycle, resulting in lift generation. These results are used to predict the motion of a cell close to the outer edge of the cell-rich core region that is generated when blood flows in a narrow tube. In this case, the lateral force is generated by shear-induced dispersion, resulting from cell-cell interactions in a region with a concentration gradient. This force is estimated using previous data on shear-induced dispersion. The cell is predicted to execute tank-treading motions at normal physiological hematocrit levels, with the possibility of tumbling at lower hematocrit levels.

Similarity scaling of turbulence in small temperate lake: implication for gas flux: implication for gas flux

NASA Astrophysics Data System (ADS)

Tedford, E. W.; MacIntyre, S.; Miller, S. D.; Czikowsky, M. J.

2013-12-01

The actively mixing layer, or surface layer, is the region of the upper mixed layer of lakes, oceans and the atmosphere directly influenced by wind, heating and cooling. Turbulence within the surface mixing layer has a direct impact on important ecological processes. The Monin-Obukhov length scale (LMO) is a critical length scale used in predicting and understanding turbulence in the actively mixed layer. On the water side of the air-water interface, LMO is defined as: LMO=-u*^3/(0.4 JB0) where u*, the shear velocity, is defined as (τ/rho)^0.5 where τ is the shear stress and rho is the density of water and JBO is the buoyancy flux at the surface. Above the depth equal to the absolute value of the Monin-Obukhov length scale (zMO), wind shear is assumed to dominate the production of turbulent kinetic energy (TKE). Below zMO, the turbulence is assumed to be suppressed when JB0 is stabilizing (warming surface waters) and enhanced when the buoyancy flux is destabilizing (cooling surface waters). Our observed dissipations were well represented using the canonical similarity scaling equations. The Monin-Obukhov length scale was generally effective in separating the surface-mixing layer into two regions: an upper region, dominated by wind shear; and a lower region, dominated by buoyancy flux. During both heating and cooling and above a depth equal to |LMO|, turbulence was dominated by wind shear and dissipation followed law of the wall scaling although was slightly augmented by buoyancy flux during both heating and cooling. Below a depth equal to |LMO| during cooling, dissipation was nearly uniform with depth. Although distinguishing between an upper region of the actively mixing layer dominated by wind stress and a lower portion dominated by buoyancy flux is typically accurate the most accurate estimates of dissipation include the effects of both wind stress and buoyancy flux throughout the actively mixed layer. We demonstrate and discuss the impact of neglecting the non-dominant forcing (buoyancy flux above zMO and wind stress below zMO) above and below zMO.

Longitudinally polarized shear wave optical coherence elastography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Miao, Yusi; Zhu, Jiang; Qi, Li; Qu, Yueqiao; He, Youmin; Gao, Yiwei; Chen, Zhongping

2017-02-01

Shear wave measurement enables quantitative assessment of tissue viscoelasticity. In previous studies, a transverse shear wave was measured using optical coherence elastography (OCE), which gives poor resolution along the force direction because the shear wave propagates perpendicular to the applied force. In this study, for the first time to our knowledge, we introduce an OCE method to detect a longitudinally polarized shear wave that propagates along the force direction. The direction of vibration induced by a piezo transducer (PZT) is parallel to the direction of wave propagation, which is perpendicular to the OCT beam. A Doppler variance method is used to visualize the transverse displacement. Both homogeneous phantoms and a side-by-side two-layer phantom were measured. The elastic moduli from mechanical tests closely matched to the values measured by the OCE system. Furthermore, we developed 3D computational models using finite element analysis to confirm the shear wave propagation in the longitudinal direction. The simulation shows that a longitudinally polarized shear wave is present as a plane wave in the near field of planar source due to diffraction effects. This imaging technique provides a novel method for the assessment of elastic properties along the force direction, which can be especially useful to image a layered tissue.

Microviscoelasticity of the apical cell surface of human umbilical vein endothelial cells (HUVEC) within confluent monolayers.

PubMed

Feneberg, Wolfgang; Aepfelbacher, Martin; Sackmann, Erich

2004-08-01

We studied the local viscoelasticity of the apical membrane of human umbilical vein endothelial cells within confluent layers by magnetic tweezers microrheometry. Magnetic beads are coupled to various integrins by coating with fibronectin or invasin. By analyzing the deflection of beads evoked by various force scenarios we demonstrate that the cell envelope behaves as a linear viscoelastic body if forces up to 2 nN are applied for short times (<20 s) but can respond in an adaptive way if stress pulses are applied longer (>30 s). The time-dependent shear relaxation modulus G(t) exhibits three time regimes: a fast response (t < 0.05 s) where the relaxation modulus G(t) obeys a power law G(t) approximately t(-0.82+/-0.02); a plateau-like behavior (at 0.05 s < t < 0.15 s); and a slow flow-like response which is, however, partially reversible. Strain field mapping experiments with colloidal probes show that local forces induce a strain field exhibiting a range of zeta = 10 +/- 1 microm, but which could only be observed if nonmagnetic beads were coupled to the cell surface by invasin. By application of the theory of elasticity of planar bodies we estimated a surface shear modulus of 2.5 x10(-4) N/m. By assuming a thickness of the actin cortex of approximately 0.5 microm we estimate a Young modulus micro approximately 400 Pa for the apical membrane. The value agrees with a plateau modulus of an entangled or weakly cross-linked actin network of an actin concentration of 100 microM (mesh size 0.2 microm). This result together with our observation of a strong reduction of the shear modulus by the actin destabilizing agent latrunculin A suggests that the shear modulus measured by our technique is determined by the actin cortex. The effect of two ligands inducing actin stress fiber formation and centripetal contraction of cells (associated with the formation of gaps in the confluent cell monolayer) on the viscoelastic responses were studied: histamine and lysophosphatidic acid (LPA). Histamine evoked a dramatic increase of the cell stiffness by >1 order of magnitude within <30 s, which is attributed to a transient rise of the intracellular Ca(2+) level, since DMSO exerted a similar effect. The stiffening is accompanied by a concomitant rounding of the cells as observed by microinterferometry and relaxes partially in the timescale of 5 min, whereas gaps between cells close after approximately 30 min. LPA did not exert a remarkable and reproducible effect other than an occasional very weak transient increase of the shear stiffness, which shows that the gap formation activated by LPA is mediated by a different mechanism than that induced by histamine.

Plasma fibronectin stabilizes Borrelia burgdorferi–endothelial interactions under vascular shear stress by a catch-bond mechanism

PubMed Central

Niddam, Alexandra F.; Ebady, Rhodaba; Bansal, Anil; Koehler, Anne; Hinz, Boris

2017-01-01

Bacterial dissemination via the cardiovascular system is the most common cause of infection mortality. A key step in dissemination is bacterial interaction with endothelia lining blood vessels, which is physically challenging because of the shear stress generated by blood flow. Association of host cells such as leukocytes and platelets with endothelia under vascular shear stress requires mechanically specialized interaction mechanisms, including force-strengthened catch bonds. However, the biomechanical mechanisms supporting vascular interactions of most bacterial pathogens are undefined. Fibronectin (Fn), a ubiquitous host molecule targeted by many pathogens, promotes vascular interactions of the Lyme disease spirochete Borrelia burgdorferi. Here, we investigated how B. burgdorferi exploits Fn to interact with endothelia under physiological shear stress, using recently developed live cell imaging and particle-tracking methods for studying bacterial–endothelial interaction biomechanics. We found that B. burgdorferi does not primarily target insoluble matrix Fn deposited on endothelial surfaces but, instead, recruits and induces polymerization of soluble plasma Fn (pFn), an abundant protein in blood plasma that is normally soluble and nonadhesive. Under physiological shear stress, caps of polymerized pFn at bacterial poles formed part of mechanically loaded adhesion complexes, and pFn strengthened and stabilized interactions by a catch-bond mechanism. These results show that B. burgdorferi can transform a ubiquitous but normally nonadhesive blood constituent to increase the efficiency, strength, and stability of bacterial interactions with vascular surfaces. Similar mechanisms may promote dissemination of other Fn-binding pathogens. PMID:28396443

Computational Study of Surface Tension and Wall Adhesion Effects on an Oil Film Flow Underneath an Air Boundary Layer

NASA Technical Reports Server (NTRS)

Celic, Alan; Zilliac, Gregory G.

1998-01-01

The fringe-imaging skin friction (FISF) technique, which was originally developed by D. J. Monson and G. G. Mateer at Ames Research Center and recently extended to 3-D flows, is the most accurate skin friction measurement technique currently available. The principle of this technique is that the skin friction at a point on an aerodynamic surface can be determined by measuring the time-rate-of-change of the thickness of an oil drop placed on the surface under the influence of the external air boundary layer. Lubrication theory is used to relate the oil-patch thickness variation to shear stress. The uncertainty of FISF measurements is estimated to be as low as 4 percent, yet little is known about the effects of surface tension and wall adhesion forces on the measured results. A modified version of the free-surface Navier-Stokes solver RIPPLE, developed at Los Alamos National Laboratories, was used to compute the time development of an oil drop on a surface under a simulated air boundary layer. RIPPLE uses the volume of fluid method to track the surface and the continuum surface force approach to model surface tension and wall adhesion effects. The development of an oil drop, over a time period of approximately 4 seconds, was studied. Under the influence of shear imposed by an air boundary layer, the computed profile of the drop rapidly changes from its initial circular-arc shape to a wedge-like shape. Comparison of the time-varying oil-thickness distributions computed using RIPPLE and also computed using a greatly simplified numerical model of an oil drop equation which does not include surface tension and wall adhesion effects) was used to evaluate the effects of surface tension on FISF measurement results. The effects of surface tension were found to be small but not necessarily negligible in some cases.

Predictors of proximal tibia anterior shear force during a vertical stop-jump.

PubMed

Sell, Timothy C; Ferris, Cheryl M; Abt, John P; Tsai, Yung-Shen; Myers, Joseph B; Fu, Freddie H; Lephart, Scott M

2007-12-01

Anterior cruciate ligament (ACL) continues to be a significant medical issue for athletes participating in sports and recreational activities. Biomechanical analyses have determined that anterior shear force is the most direct loading mechanism of the ACL and a probable component of noncontact ACL injury. The purpose of this study was to examine the biomechanical predictors of proximal tibia anterior shear force during a stop-jump task. A biomechanical and electromyographic (EMG) analysis of the knee was conducted while subjects performed a vertical stop-jump task. The task was chosen to simulate an athletic maneuver that included a landing with a sharp deceleration and a change in direction. The final regression model indicated that posterior ground reaction force, external knee flexion moment, knee flexion angle, integrated EMG activity of the vastus lateralis, and sex (female) would significantly predict proximal tibia anterior shear force (p < 0.0001, R2 = 0.8609). Knee flexion moment had the greatest influence on proximal tibia anterior shear force. The mathematical relationships elucidated in the current study support previous clinical and basic science research examining noncontact ACL injuries. This data provides important evidence for clinicians who are examining the risk factors for these injuries and developing/validating training programs to reduce the incidence of injury. Copyright 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Contact force structure and force chains in 3D sheared granular systems

NASA Astrophysics Data System (ADS)

Mair, Karen; Jettestuen, Espen; Abe, Steffen

2010-05-01

Faults often exhibit accumulations of granular debris, ground up to create a layer of rock flour or fault gouge separating the rigid fault walls. Numerical simulations and laboratory experiments of sheared granular materials, suggest that applied loads are preferentially transmitted across such systems by transient force networks that carry enhanced forces. The characterisation of such features is important since their nature and persistence almost certainly influence the macroscopic mechanical stability of these systems and potentially that of natural faults. 3D numerical simulations of granular shear are a valuable investigation tool since they allow us to track individual particle motions, contact forces and their evolution during applied shear, that are difficult to view directly in laboratory experiments or natural fault zones. In characterising contact force distributions, it is important to use global structure measures that allow meaningful comparisons of granular systems having e.g. different grain size distributions, as may be expected at different stages of a fault's evolution. We therefore use a series of simple measures to characterise the structure, such as distributions and correlations of contact forces that can be mapped onto a force network percolation problem as recently proposed by Ostojic and coworkers for 2D granular systems. This allows the use of measures from percolation theory to both define and characterise the force networks. We demonstrate the application of this method to 3D simulations of a sheared granular material. Importantly, we then compare our measure of the contact force structure with macroscopic frictional behaviour measured at the boundaries of our model to determine the influence of the force networks on macroscopic mechanical stability.

On the Influence of Surface Heterogeneities onto Roll Convection

NASA Astrophysics Data System (ADS)

Gryschka, M.; Drüe, C.; Raasch, S.; Etling, D.

2009-04-01

Roll convection is a common phenomenon in atmospheric convective boundary layers (CBL) with background wind. Roll convection is observed both over land and over sea for different synoptic situations. There is still some debate about the different types of roll convection and their causes or rather the necessary conditions for their appearance. The stability parameter ζ = -zi•L (zi: boundary layer height, L: Monin-Obukhov stability length) is widely used as a predictor for roll convection, since numerous studies suggest that convective rolls only appear when 0 < ζ < 20. In other words, roll development becomes unlikely for strong surface heating and weak vertical wind shear. In contrast to those studies the presence of roll convection in almost any polar cold air outbreak (as can be seen in numerous satellite images as cloud streets) reveals that even for large ζ roll convection can develop. Some studies report roll convection in cold air outbreaks for ζ = 250. Our large eddy simulations (LES) on roll convection suggests that the contrasting results concerning the dependency of roll convection on ζ are due to two different types of roll convection: One type which develops purely by self organization if ζ < 20 ("free rolls") and another type which is triggered by heterogeneities in surface temperature and develops also for large ζ ("forced rolls"). We think that most of the cloud streets observed in polar cold air outbreaks over open water are due to rolls of forced type which are tied to upstream located heterogeneities in the sea-ice distribution. The results of this study suggests that the omission of surface inhomogeneities in previous LES is the reason for the absence of rolls in all LES with strong surface heating and weak vertical wind shear so far. In this contribution we will present a large eddy simulation which successfully represents forced rolls under such conditions.

Simulation of synthetic gecko arrays shearing on rough surfaces

PubMed Central

Gillies, Andrew G.; Fearing, Ronald S.

2014-01-01

To better understand the role of surface roughness and tip geometry in the adhesion of gecko synthetic adhesives, a model is developed that attempts to uncover the relationship between surface feature size and the adhesive terminal feature shape. This model is the first to predict the adhesive behaviour of a plurality of hairs acting in shear on simulated rough surfaces using analytically derived contact models. The models showed that the nanoscale geometry of the tip shape alters the macroscale adhesion of the array of fibres by nearly an order of magnitude, and that on sinusoidal surfaces with amplitudes much larger than the nanoscale features, spatula-shaped features can increase adhesive forces by 2.5 times on smooth surfaces and 10 times on rough surfaces. Interestingly, the summation of the fibres acting in concert shows behaviour much more complex that what could be predicted with the pull-off model of a single fibre. Both the Johnson–Kendall–Roberts and Kendall peel models can explain the experimentally observed frictional adhesion effect previously described in the literature. Similar to experimental results recently reported on the macroscale features of the gecko adhesive system, adhesion drops dramatically when surface roughness exceeds the size and spacing of the adhesive fibrillar features. PMID:24694893

Activation of biceps femoris long head reduces tibiofemoral anterior shear force and tibial internal rotation torque in healthy subjects

PubMed Central

Azmi, Nur Liyana; Ding, Ziyun; Xu, Rui

2018-01-01

The anterior cruciate ligament (ACL) provides resistance to tibial internal rotation torque and anterior shear at the knee. ACL deficiency results in knee instability. Optimisation of muscle contraction through functional electrical stimulation (FES) offers the prospect of mitigating the destabilising effects of ACL deficiency. The hypothesis of this study is that activation of the biceps femoris long head (BFLH) reduces the tibial internal rotation torque and the anterior shear force at the knee. Gait data of twelve healthy subjects were measured with and without the application of FES and taken as inputs to a computational musculoskeletal model. The model was used to investigate the optimum levels of BFLH activation during FES gait in reducing the anterior shear force to zero. This study found that FES significantly reduced the tibial internal rotation torque at the knee during the stance phase of gait (p = 0.0322) and the computational musculoskeletal modelling revealed that a mean BFLH activation of 20.8% (±8.4%) could reduce the anterior shear force to zero. At the time frame when the anterior shear force was zero, the internal rotation torque was reduced by 0.023 ± 0.0167 Nm/BW, with a mean 188% reduction across subjects (p = 0.0002). In conclusion, activation of the BFLH is able to reduce the tibial internal rotation torque and the anterior shear force at the knee in healthy control subjects. This should be tested on ACL deficient subject to consider its effect in mitigating instability due to ligament deficiency. In future clinical practice, activating the BFLH may be used to protect ACL reconstructions during post-operative rehabilitation, assist with residual instabilities post reconstruction, and reduce the need for ACL reconstruction surgery in some cases. PMID:29304102

Activation of biceps femoris long head reduces tibiofemoral anterior shear force and tibial internal rotation torque in healthy subjects.

PubMed

Azmi, Nur Liyana; Ding, Ziyun; Xu, Rui; Bull, Anthony M J

2018-01-01

The anterior cruciate ligament (ACL) provides resistance to tibial internal rotation torque and anterior shear at the knee. ACL deficiency results in knee instability. Optimisation of muscle contraction through functional electrical stimulation (FES) offers the prospect of mitigating the destabilising effects of ACL deficiency. The hypothesis of this study is that activation of the biceps femoris long head (BFLH) reduces the tibial internal rotation torque and the anterior shear force at the knee. Gait data of twelve healthy subjects were measured with and without the application of FES and taken as inputs to a computational musculoskeletal model. The model was used to investigate the optimum levels of BFLH activation during FES gait in reducing the anterior shear force to zero. This study found that FES significantly reduced the tibial internal rotation torque at the knee during the stance phase of gait (p = 0.0322) and the computational musculoskeletal modelling revealed that a mean BFLH activation of 20.8% (±8.4%) could reduce the anterior shear force to zero. At the time frame when the anterior shear force was zero, the internal rotation torque was reduced by 0.023 ± 0.0167 Nm/BW, with a mean 188% reduction across subjects (p = 0.0002). In conclusion, activation of the BFLH is able to reduce the tibial internal rotation torque and the anterior shear force at the knee in healthy control subjects. This should be tested on ACL deficient subject to consider its effect in mitigating instability due to ligament deficiency. In future clinical practice, activating the BFLH may be used to protect ACL reconstructions during post-operative rehabilitation, assist with residual instabilities post reconstruction, and reduce the need for ACL reconstruction surgery in some cases.

Estimating force and power requirements for crosscut shearing of roundwood.

Treesearch

Rodger A. Arola

1972-01-01

Presents a procedure which, through the use of nomographs, permits rapid estimation of the force required to crosscut shear logs of various species and diameters with shear blades ranging in thickness from 1/4 to 7/8 inch. In addition, nomographs are included to evaluate hydraulic cylinder sizes, pump capacities, and motor horsepower requirements to effect the cut....

Dissolved oxygen as a key parameter to aerobic granule formation.

PubMed

Sturm, B S McSwain; Irvine, R L

2008-01-01

Much research has asserted that high shear forces are necessary for the formation of aerobic granular sludge in Sequencing Batch Reactors (SBRs). In order to distinguish the role of shear and dissolved oxygen on granule formation, two separate experiments were conducted with three bench-scale SBRs. In the first experiment, an SBR was operated with five sequentially decreasing superficial upflow gas velocities ranging from 1.2 to 0.4 cm s(-1). When less than 1 cm s(-1) shear was applied to the reactor, aerobic granules disintegrated into flocs, with corresponding increases in SVI and effluent suspended solids. However, the dissolved oxygen also decreased from 8 mg L(-1) to 5 mg L(-1), affecting the Feast/Famine regime in the SBR and the substrate removal kinetics. A second experiment operated two SBRs with an identical shear force of 1.2 cm s(-1), but two dissolved oxygen concentrations. Even when supplied a high shear force, aerobic granules could not form at a dissolved oxygen less than 5 mg L(-1), with a Static Fill. These results indicate that the substrate removal kinetics and dissolved oxygen are more significant to granule formation than shear force. Copyright IWA Publishing 2008.

Measurement of the Shear Lift Force on a Bubble in a Channel Flow

NASA Technical Reports Server (NTRS)

Nahra, Henry K.; Motil, Brian; Skor, Mark

2005-01-01

Two-phase flow systems play vital roles in the design of some current and anticipated space applications of two-phase systems which include: thermal management systems, transfer line flow in cryogenic storage, space nuclear power facilities, design and operation of thermal bus, life support systems, propulsion systems, In Situ Resource Utilization (ISRU), and space processes for pharmaceutical applications. The design of two-phase flow systems for space applications requires a clear knowledge of the behaviors of the dispersed phase (bubble), its interaction with the continuous phase (liquid) and its effect on heat and mass transfer processes, The need to understand the bubble generation process arises from the fact that for all space applications, the size and distribution of bubbles are extremely crucial for heat and mass transfer control. One important force in two-phase flow systems is the lift force on a bubble or particle in a liquid shear flow. The shear lift is usually overwhelmed by buoyancy in normal gravity, but it becomes an important force in reduced gravity. Since the liquid flow is usually sheared because of the confining wall, the trajectories of bubbles and particles injected into the liquid flow are affected by the shear lift in reduced gravity. A series of experiments are performed to investigate the lift force on a bubble in a liquid shear flow and its effect on the detachment of a bubble from a wall under low gravity conditions. Experiments are executed in a Poiseuille flow in a channel. An air-water system is used in these experiments that are performed in the 2.2 second drop tower. A bubble is injected into the shear flow from a small injector and the shear lift is measured while the bubble is held stationary relative to the fluid. The trajectory of the bubble prior, during and after its detachment from the injector is investigated. The measured shear lift force is calculated from the trajectory of the bubble at the detachment point. These values for the shear lift are then compared with the theoretical predictions from various published works on shear lift in the open literature, which include asymptotic solutions at low bubble Reynolds number, potential flow predictions and numerical studies that deal with intermediate bubble Reynolds numbers.

GPU-based Green’s function simulations of shear waves generated by an applied acoustic radiation force in elastic and viscoelastic models

NASA Astrophysics Data System (ADS)

Yang, Yiqun; Urban, Matthew W.; McGough, Robert J.

2018-05-01

Shear wave calculations induced by an acoustic radiation force are very time-consuming on desktop computers, and high-performance graphics processing units (GPUs) achieve dramatic reductions in the computation time for these simulations. The acoustic radiation force is calculated using the fast near field method and the angular spectrum approach, and then the shear waves are calculated in parallel with Green’s functions on a GPU. This combination enables rapid evaluation of shear waves for push beams with different spatial samplings and for apertures with different f/#. Relative to shear wave simulations that evaluate the same algorithm on an Intel i7 desktop computer, a high performance nVidia GPU reduces the time required for these calculations by a factor of 45 and 700 when applied to elastic and viscoelastic shear wave simulation models, respectively. These GPU-accelerated simulations also compared to measurements in different viscoelastic phantoms, and the results are similar. For parametric evaluations and for comparisons with measured shear wave data, shear wave simulations with the Green’s function approach are ideally suited for high-performance GPUs.

Direct measurements of local bed shear stress in the presence of pressure gradients

NASA Astrophysics Data System (ADS)

Pujara, Nimish; Liu, Philip L.-F.

2014-07-01

This paper describes the development of a shear plate sensor capable of directly measuring the local mean bed shear stress in small-scale and large-scale laboratory flumes. The sensor is capable of measuring bed shear stress in the range 200 Pa with an accuracy up to 1 %. Its size, 43 mm in the flow direction, is designed to be small enough to give spatially local measurements, and its bandwidth, 75 Hz, is high enough to resolve time-varying forcing. Typically, shear plate sensors are restricted to use in zero pressure gradient flows because secondary forces on the edge of the shear plate caused by pressure gradients can introduce large errors. However, by analysis of the pressure distribution at the edges of the shear plate in mild pressure gradients, we introduce a new methodology for correcting for the pressure gradient force. The developed sensor includes pressure tappings to measure the pressure gradient in the flow, and the methodology for correction is applied to obtain accurate measurements of bed shear stress under solitary waves in a small-scale wave flume. The sensor is also validated by measurements in a turbulent flat plate boundary layer in open channel flow.

Development of living cell force sensors for the interrogation of cell surface interactions

NASA Astrophysics Data System (ADS)

Brown, Scott Chang

The measurement of cell surface interactions, or cell interaction forces, are critical for the early diagnosis and prevention of disease, the design of targeted drug and gene delivery vehicles, the development of next-generation implant materials, and much more. However, the technologies and devices that are currently available are highly limited with respect to the dynamic force range over which they can measure cell-cell or cell-substratum interactions, and with their ability to adequately mimic biologically relevant systems. Consequently, research efforts that involve cell surface interactions have been limited. In this dissertation, existing tools for research at the nanoscale (i.e., atomic force microscopy microcantilevers) are modified to develop living cell force sensors that allow for the highly sensitive measurement of cell-mediated interactions over the entire range of forces expected in biotechnology (and nano-biotechnology) research (from a single to millions of receptor-ligand bonds). Several force sensor motifs have been developed that can be used to measure interactions using single adherent cells, single suspension culture cell, and cell monolayers (tissues) over a wide range of interaction conditions (e.g., approach velocity, shear rate, contact time) using a conventional atomic force microscope. This new tool has been applied to study the pathogenesis of spontaneous pneumothorax and the interaction of cells with 14 man-made interfaces. Consequently, a new hypothesis of the interactions that manifest spontaneous pneumothorax has been developed. Additionally, these findings have the potential to lead to the development of tools for data mining materials and surfaces for unique cell interactions that could have an immense societal impact.

The kinematics and initiation mechanisms of the earthquake-triggered Daguangbao landslide

NASA Astrophysics Data System (ADS)

Yang, Che-Ming; Cheng, Hui-Yun; Tsao, Chia-Che; Wu, Wen-Jie; Dong, Jia-Jyun; Lee, Chyi-Tyi; Lin, Ming-Lang; Zhang, Wei-Fong; Pei, Xiang-Jun; Wang, Gong-Hui; Huang, Run-Qiu

2015-04-01

The Daguangbao (DGB) landslide is one of the largest earthquake-triggered landslides induced by the 2008 Wenchuan earthquake in the world over the past century. Based on remote sensing images, topography analysis and field investigation, this landslide was speculated a gigantic atypical wedge failure with the folded bedding plane and a zigzag stepping-out joint system, which outcropped at the south and north, respectively. With the inferred failure surfaces, the volume of the DGB landslide is about 1,051 Mm3. The frequently adopted Rigid Wedge Method (RWM), which assumed zero shear stress on the sliding surface along the vectors perpendicular to the intersection line when evaluating the wedge stability, could not be valid for this super large DGB wedge. Under an assumption that the shear strength is fully mobilized on the sliding surface along the vectors perpendicular to the intersection line, this study proposed to use a Maximum Shear Stress Method (MSSM) to calculate the factor of safety (FOS) of the DGB wedge. Based on the assumptions of the two methods, the FOS of the RWM and MSSM are the upper and lower bounds for the wedge stability analysis. Based on the rotary shear tests, the averaged friction coefficients of the representative materials of the two sliding surfaces are 0.79 (bedding parallel fault gauges) and 0.71 (dolomite joints). Without external force, the FOSs of the DGB landslide are 4.14 and 2.51 by the RWM and MSSM, respectively. Restate, the wedge is stable before the 2008 Wenchuan earthquake. However, DGB landslide can be triggered at 35.7 sec based on the ground acceleration records of strong motion station MZQP during the 2008 Wenchuan earthquake and the pseudo-static stability analysis incorporated into MSSM (Acceleration: EW=0.272g, NS=0.152g, Vertical=0.244g). Moreover, using the friction coefficient of the representative materials under large shear displacement under shear velocity of 1.3 m/s (0.16 for bedding parallel fault gouges and 0.1 for dolomite joints), the gigantic wedge can be speeded up to a maximum velocity of 54 m/sec. The traveled time will be 70 seconds with a travel distance of 1.9 km.

Architectural evidence of dune collapse in the Navajo Sandstone, Zion National Park, Utah

NASA Astrophysics Data System (ADS)

Ford, Colby; Bryant, Gerald; Nick, Kevin E.

2016-10-01

The Canyon Overlook Trail of Zion National Park follows an outcrop of Navajo Sandstone, which displays a uniquely well-exposed assemblage of features associated with failure of the lee face of a large eolian dune, and run-out over an expanse of interdune sediments downwind of that bedform. Exposed features include dramatic folds in the interdune succession and a stacked series of thrust sheets incorporating both interdune and overlying dune deposits. Thrust surfaces display consistent strikes, parallel to those of undeformed foresets, and incorporate zones of brittle failure and fluid deformation, including folds overturned in the direction of foreset dip. These features correspond to predictions made by a previous researcher's model of dune collapse, formulated from less fortuitously exposed architectures in the Navajo Sandstone. Unlike the previous model, however, this site preserves distinct indications that the bulk of deformed material accumulated above the level of the contemporary interdune surface, in an aggradational succession. Paleotopographic reconstruction, based on preserved facies relationships at this site, indicates the presence of a large dune, partially encroached upon a well-developed wet interdune succession, made up of two half-meter carbonate mud layers, separated by a meter of medium-grained sand. Trapping of pore water pressure between these mud layers during liquefaction reduced shear strength in this interval, facilitating the collapse of the lee face of the upwind dune into the interdune area, and transmitted resultant shear forces to distal portions of the interdune expanse, in the shallow subsurface. Shear failure developed along bedding planes in the horizontally laminated carbonate muds, which provided both lubrication of the shear surfaces and structural support for the preservation of coherent thrust sheets during production of an imbricated succession of shear zones in the toe portion of the slump. Individual shear surfaces exposed in this outcrop extend for up to 50 m along strike and dip north up to 55°. Upturned mud layers in the toe of the slump resisted deflation, promoting preservation of an irregular interdune topography, over which the reorganized dune ultimately advanced.

Hydromechanical coupling in fractured rock masses: mechanisms and processes of selected case studies

NASA Astrophysics Data System (ADS)

Zangerl, Christian

2015-04-01

Hydromechanical (HM) coupling in fractured rock play an important role when events including dam failures, landslides, surface subsidences due to water withdrawal or drainage, injection-induced earthquakes and others are analysed. Generally, hydromechanical coupling occurs when a rock mass contain interconnected pores and fractures which are filled with water and pore/fracture pressures evolves. In the on hand changes in the fluid pressure can lead to stress changes, deformations and failures of the rock mass. In the other hand rock mass stress changes and deformations can alter the hydraulic properties and fluid pressures of the rock mass. Herein well documented case studies focussing on surface subsidence due to water withdrawal, reversible deformations of large-scale valley flanks and failure as well as deformation processes of deep-seated rock slides in fractured rock masses are presented. Due to pore pressure variations HM coupling can lead to predominantly reversible rock mass deformations. Such processes can be considered by the theory of poroelasticity. Surface subsidence reaching magnitudes of few centimetres and are caused by water drainage into deep tunnels are phenomenas which can be assigned to processes of poroelasticity. Recently, particular focus was given on large tunnelling projects to monitor and predict surface subsidence in fractured rock mass in oder to avoid damage of surface structures such as dams of large reservoirs. It was found that surface subsidence due to tunnel drainage can adversely effect infrastructure when pore pressure drawdown is sufficiently large and spatially extended and differential displacements which can be amplified due to topographical effects e.g. valley closure are occurring. Reversible surface deformations were also ascertained on large mountain slopes and summits with the help of precise deformation measurements i.e. permanent GPS or episodic levelling/tacheometric methods. These reversible deformations are often in the range of millimetres to a very few centimetres and can be linked to annual groundwater fluctuations. Due to pore pressure variations HM coupling can influence seepage forces and effective stresses in the rock mass. Effective stress changes can adversely affect the stability and deformation behaviour of deep-seated rock slides by influencing the shear strength or the time dependent (viscous) material behaviour of the basal shear zone. The shear strength of active shear zones is often reasonably well described by Coulomb's law. In Coulomb's law the operative normal stresses to the shear surface/zone are effective stresses and hence pore pressures which should be taken into account reduces the shear strength. According to the time dependent material behaviour a few effective stress based viscous models exists which are able to consider pore pressures. For slowly moving rock slides HM coupling could be highly relevant when low-permeability clayey-silty shear zones (fault gouges) are existing. An important parameters therefore is the hydraulic diffusivity, which is controlled by the permeability and fluid-pore compressibility of the shear zone, and by fluid viscosity. Thus time dependent pore pressure diffusion in the shear zone can either control the stability condition or the viscous behaviour (creep) of the rock slide. Numerous cases studies show that HM coupling can effect deformability, shear strength and time dependent behaviour of fractured rock masses. A process-based consideration can be important to avoid unexpected impacts on infrastructures and to understand complex rock mass as well rock slide behaviour.

Fast Low-to-High Confinement Mode Bifurcation Dynamics in a Tokamak Edge Plasma Gyrokinetic Simulation.

PubMed

Chang, C S; Ku, S; Tynan, G R; Hager, R; Churchill, R M; Cziegler, I; Greenwald, M; Hubbard, A E; Hughes, J W

2017-04-28

Transport barrier formation and its relation to sheared flows in fluids and plasmas are of fundamental interest in various natural and laboratory observations and of critical importance in achieving an economical energy production in a magnetic fusion device. Here we report the first observation of an edge transport barrier formation event in an electrostatic gyrokinetic simulation carried out in a realistic diverted tokamak edge geometry under strong forcing by a high rate of heat deposition. The results show that turbulent Reynolds-stress-driven sheared E×B flows act in concert with neoclassical orbit loss to quench turbulent transport and form a transport barrier just inside the last closed magnetic flux surface.

New views of granular mass flows

USGS Publications Warehouse

Iverson, R.M.; Vallance, J.W.

2001-01-01

Concentrated grain-fluid mixtures in rock avalanches, debris flows, and pyroclastic flows do not behave as simple materials with fixed rheologies. Instead, rheology evolves as mixture agitation, grain concentration, and fluid-pressure change during flow initiation, transit, and deposition. Throughout a flow, however, normal forces on planes parallel to the free upper surface approximately balance the weight of the superincumbent mixture, and the Coulomb friction rule describes bulk intergranular shear stresses on such planes. Pore-fluid pressure can temporarily or locally enhance mixture mobility by reducing Coulomb friction and transferring shear stress to the fluid phase. Initial conditions, boundary conditions, and grain comminution and sorting can influence pore-fluid pressures and cause variations in flow dynamics and deposits.

Influence of trabecular bone quality and implantation direction on press-fit mechanics.

PubMed

Damm, Niklas B; Morlock, Michael M; Bishop, Nicholas E

2017-02-01

Achieving primary stability of uncemented press-fit prostheses in patients with poor quality bone can involve axial implantation forces large enough to cause bone fracture. Radial implantation eliminates intraoperative impaction forces and could prevent this damage. Platens of two commercial implant surfaces ("Beaded" and "Flaked") were implanted onto trabecular bone specimens of varying quality in a press-fit simulator. Samples were implanted with varying interference, either axially (shear) or radially (normal). Push-in and pull-out forces were measured to assess stability. Microstructural changes in the bone were determined from μCT analysis. For force-defined implantation analysis, push-in and pull-out forces both increased proportionally with increasing radial force, independent of implantation direction, bone quality or implant surface. For position-defined implantation analysis, pull-out forces were generally found to increase with interference and to be greater for radial than axial implantation direction, and to be lower for poor quality bone. Bone density increased locally at the tested interface due to implantation, in particular for the Beaded surface under axial implantation. If a safe radial stress can be determined for cortical bone in a particular patient, the associated implantation force, and pull-out force which represents primary stability, can be directly derived, regardless of implantation direction, bone quality or implant surface. Radial implantation delivers primary stability that is no worse than that for axial implantation and may eliminate potentially damaging impaction forces. Development of implant designs based on this principal might improve implant fixation. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:224-233, 2017. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Prevalence of low back disorders among female workers and biomechanical limits on the handling of load and patients.

PubMed

Gutiérrez, Manuel; Monzó, Jorge

2012-01-01

The purpose of this investigation was to determine the association between prevalence of low back disorders in female workers and biomechanical demands of compressive and shear forces at the lumbar spine. A descriptive, cross-sectional and correlational study was carried out in 11 groups of female workers in the Province of Concepción. An interview was performed to investigate the prevalence of low back pain. To estimate biomechanical demands on the lumbar spine, it was used the 3DSSPP software. The Pearson correlation coefficient between the prevalence of low back disorders and peak compression force at the lumbar spine was r = (p<0.005). The Spearman correlation coefficient between the prevalence of low back disorders and peak shear force was r = 0.9 (p <0.005). To protect 90% of female workers studied, the limits of compression and shear forces should be at 2.8 kN and 0.3 kN, respectively. These values differ from the recommendations currently used, 3.4 kN for peak compression force and 0.5 kN for peak shear force.

The effect of inoculum source and fluid shear force on the development of in vitro oral multispecies biofilms.

PubMed

Fernández, C E; Aspiras, M B; Dodds, M W; González-Cabezas, C; Rickard, A H

2017-03-01

Saliva has been previously used as an inoculum for in vitro oral biofilm studies. However, the microbial community profile of saliva is markedly different from hard- and soft-tissue-associated oral biofilms. Here, we investigated the changes in the biofilm architecture and microbial diversity of in vitro oral biofilms developed from saliva, tongue or plaque-derived inocula under different salivary shear forces. Four inoculum types (saliva, bacteria harvested from the tongue, toothbrush and curette-harvested plaque) were collected and pooled. Biofilms (n ≥ 15) were grown for 20 h in cell-free human saliva flowing at three different shear forces. Stained biofilms were imaged using a confocal laser scanning microscope. Biomass, thickness and roughness were determined by image analysis and bacterial community composition analysed using Ion Torrent. All developed biofilms showed a significant reduction in observed diversity compared with their respective original inoculum. Shear force altered biofilm architecture of saliva and curette-collected plaque and community composition of saliva, tongue and curette-harvested plaque. Different intraoral inocula served as precursors of in vitro oral polymicrobial biofilms which can be influenced by shear. Inoculum selection and shear force are key factors to consider when developing multispecies biofilms within in vitro models. © 2016 The Society for Applied Microbiology.

Virtual surface characteristics of a tactile display using magneto-rheological fluids.

PubMed

Lee, Chul-Hee; Jang, Min-Gyu

2011-01-01

Virtual surface characteristics of tactile displays are investigated to characterize the feeling of human touch for a haptic interface application. In order to represent the tactile feeling, a prototype tactile display incorporating Magneto-Rheological (MR) fluid has been developed. Tactile display devices simulate the finger's skin to feel the sensations of contact such as compliance, friction, and topography of the surface. Thus, the tactile display can provide information on the surface of an organic tissue to the surgeon in virtual reality. In order to investigate the compliance feeling of a human finger's touch, normal force responses of a tactile display under various magnetic fields have been assessed. Also, shearing friction force responses of the tactile display are investigated to simulate the action of finger dragging on the surface. Moreover, different matrix arrays of magnetic poles are applied to form the virtual surface topography. From the results, different tactile feelings are observed according to the applied magnetic field strength as well as the arrays of magnetic poles combinations. This research presents a smart tactile display technology for virtual surfaces.

L4-L5 compression and anterior/posterior joint shear forces in cabin attendants during the initial push/pull actions of airplane meal carts.

PubMed

Sandfeld, Jesper; Rosgaard, Christian; Jensen, Bente Rona

2014-07-01

The aim of the present study was to assess the acute low back load of cabin attendants during cart handling and to identify working situations which present the highest strain on the worker. In a setup, 17 cabin attendants (ten females and seven males) pushed, pulled and turned a 20 kg standard meal cart (L: 0.5m × W: 0.3 m × H: 0.92 m) loaded with extra 20 kg and 40 kg, respectively on two different surfaces (carpet and linoleum) and at three floor inclinations (-2°, 0° and +2°). Two force transducers were mounted as handles. Two-dimensional movement analysis was performed and a 4D WATBAK modelling tool was used to calculate the acute L4-L5 load. No working situations created loads greater than the accepted values for single exertions, however compression and anterior/posterior shear forces during pulling and turning were much higher when compared with pushing. There were significant effects of handling the cart on different floor types, at the varying inclinations and with different cart weights. Additionally, when external forces were reduced, the cabin attendants did not decrease push/pull force proportionally and thus the L4-L5 load did not decrease as much as expected. Copyright © 2014 Elsevier Ltd and The Ergonomics Society. All rights reserved.

Restoration of the contact surface in FORCE-type centred schemes I: Homogeneous two-dimensional shallow water equations

NASA Astrophysics Data System (ADS)

Canestrelli, Alberto; Toro, Eleuterio F.

2012-10-01

Recently, the FORCE centred scheme for conservative hyperbolic multi-dimensional systems has been introduced in [34] and has been applied to Euler and relativistic MHD equations, solved on unstructured meshes. In this work we propose a modification of the FORCE scheme, named FORCE-Contact, that provides improved resolution of contact and shear waves. This paper presents the technique in full detail as applied to the two-dimensional homogeneous shallow water equations. The improvements due to the new method are particularly evident when an additional equation is solved for a tracer, since the modified scheme exactly resolves isolated and steady contact discontinuities. The improvement is considerable also for slowly moving contact discontinuities, for shear waves and for steady states in meandering channels. For these types of flow fields, the numerical results provided by the new FORCE-Contact scheme are comparable with, and sometimes better than, the results obtained from upwind schemes, such as Roes scheme for example. In a companion paper, a similar approach to restoring the missing contact wave and preserving well-balanced properties for non-conservative one- and two-layer shallow water equations is introduced. However, the procedure is general and it is in principle applicable to other multidimensional hyperbolic systems in conservative and non-conservative form, such as the Euler equations for compressible gas dynamics.

Contributions of Nanodiamond Abrasives and Deionized Water in Magnetorheological Finishing of Aluminum Oxynitride

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miao, C.; Lambropoulos, J.C.; Romanofsky, H.

2010-01-13

Magnetorheological finishing (MRF) is a sub-aperture deterministic process for fabricating high-precision optics by removing material and smoothing the surface. The goal of this work is to study the relative contribution of nanodiamonds and water in material removal for MRF of aluminum oxynitride ceramic (ALON) based upon a nonaqueous magnetorheological (MR) fluid. Removal was enhanced by a high carbonyl iron concentration and the addition of nanodiamond abrasives. Small amounts of deionized (DI) water were introduced into the nonaqueous MR fluid to further influence the material removal process. Material removal data were collected with a spot-taking machine. Drag force (Fd) and normalmore » force (Fn) before and after adding nanodiamonds or DI water were measured with a dual load cell. Both drag force and normal force were insensitive to the addition of nanodiamonds but increased with DI water content in the nonaqueous MR fluid. Shear stress (i.e., drag force divided by spot area) was calculated, and examined as a function of nanodiamond concentration and DI water concentration. Volumetric removal rate increased with increasing shear stress, which was shown to be a result of increasing viscosity after adding nanodiamonds and DI water. This work demonstrates that removal rate for a hard ceramic with MRF can be enhanced by adding DI water into a nonaqueous MR fluid.« less

Rolling process for producing biaxially textured substrates

DOEpatents

Goyal, Amit

2004-05-25

A method of preparing a biaxially textured article includes the steps of: rolling a metal preform while applying shear force thereto to form as-rolled biaxially textured substrate having an a rotated cube texture wherein a (100) cube face thereof is parallel to a surface of said substrate, and wherein a [100] direction thereof is at an angle of at least 30.degree. relative to the rolling direction; and depositing onto the surface of the biaxially textured substrate at least one epitaxial layer of another material to form a biaxially textured article.

The impact of pH on floc structure characteristic of polyferric chloride in a low DOC and high alkalinity surface water treatment.

PubMed

Cao, Baichuan; Gao, Baoyu; Liu, Xin; Wang, Mengmeng; Yang, Zhonglian; Yue, Qinyan

2011-11-15

The adjustment of pH is an important way to enhance removal efficiency in coagulation units, and in this process, the floc size, strength and structure can be changed, influencing the subsequent solid/liquid separation effect. In this study, an inorganic polymer coagulant, polyferric chloride (PFC) was used in a low dissolved organic carbon (DOC) and high alkalinity surface water treatment. The influence of coagulation pH on removal efficiency, floc growth, strength, re-growth capability and fractal dimension was examined. The optimum dosage was predetermined as 0.150 mmol/L, and excellent particle and organic matter removal appeared in the pH range of 5.50-5.75. The structure characteristics of flocs formed under four pH conditions were investigated through the analysis of floc size, effect of shear and particle scattering properties by a laser scattering instrument. The results indicated that flocs formed at neutral pH condition gave the largest floc size and the highest growth rate. During the coagulation period, the fractal dimension of floc aggregates increased in the first minutes and then decreased and larger flocs generally had smaller fractal dimensions. The floc strength, which was assessed by the relationship of floc diameter and velocity gradient, decreased with the increase of coagulation pH. Flocs formed at pH 4.00 had better recovery capability when exposed to lower shear forces, while flocs formed at neutral and alkaline conditions had better performance under higher shear forces. Copyright © 2011 Elsevier Ltd. All rights reserved.

Assessing the Impact of Germination and Sporulation Conditions on the Adhesion of Bacillus Spores to Glass and Stainless Steel by Fluid Dynamic Gauging

PubMed Central

Xu Zhou, Ke; Li, Nan; Christie, Graham

2017-01-01

Abstract The adhesion of spores of 3 Bacillus species with distinctive morphologies to stainless steel and borosilicate glass was studied using the fluid dynamic gauging technique. Marked differences were observed between different species of spores, and also between spores of the same species prepared under different sporulation conditions. Spores of the food‐borne pathogen B. cereus were demonstrated to be capable of withstanding shear stresses greater than 1500 Pa when adhered to stainless steel, in contrast to spores of Bacillus subtilis and Bacillus megaterium, which detached in response to lower shear stress. An extended DLVO model was shown to be capable of predicting the relative differences in spore adhesion between spores of different species and different culture conditions, but did not predict absolute values of force of adhesion well. Applying the model to germinating spores showed a significant reduction in adhesion force shortly after triggering germination, indicating a potential strategy to achieve enhanced removal of spores from surfaces in response to shear stress, such as during cleaning‐in‐place procedures. Practical Application Spore‐forming bacteria are a concern to the food industry because they have the potential to cause food‐borne illness and product spoilage, while being strongly adhesive to processing surfaces and resistant to cleaning‐in‐place procedures. This work is of significance to the food processors and manufacturers because it offers insight to the properties of spore adhesion and identifies a potential strategy to facilitate the removal of spores during cleaning procedures. PMID:29125641

On multiscale moving contact line theory.

PubMed

Li, Shaofan; Fan, Houfu

2015-07-08

In this paper, a multiscale moving contact line (MMCL) theory is presented and employed to simulate liquid droplet spreading and capillary motion. The proposed MMCL theory combines a coarse-grained adhesive contact model with a fluid interface membrane theory, so that it can couple molecular scale adhesive interaction and surface tension with hydrodynamics of microscale flow. By doing so, the intermolecular force, the van der Waals or double layer force, separates and levitates the liquid droplet from the supporting solid substrate, which avoids the shear stress singularity caused by the no-slip condition in conventional hydrodynamics theory of moving contact line. Thus, the MMCL allows the difference of the surface energies and surface stresses to drive droplet spreading naturally. To validate the proposed MMCL theory, we have employed it to simulate droplet spreading over various elastic substrates. The numerical simulation results obtained by using MMCL are in good agreement with the molecular dynamics results reported in the literature.

Stress hysteresis as the cause of persistent holes in particulate suspensions

NASA Astrophysics Data System (ADS)

Deegan, Robert D.

2010-03-01

Concentrated particulate suspensions under vibrations can support stable, localized, vertically oriented free surfaces. The most robust of these structures are persistent holes: deep and stable depressions of the interface. Using a reduced model of the hydrodynamics we show that a rheology with hysteresis can lead to motion opposite to the time-averaged applied force. Moreover, we show experimentally that particulate suspensions of cornstarch in water exhibits hysteresis in the shear-rate response to an applied sinusoidal stress. The results of our model and our experiments suggest that hysteresis accounts for the outward force needed to support persistent holes.

Sediment-transport (wind) experiments in zero-gravity

NASA Technical Reports Server (NTRS)

Iverson, J.; Gillette, D.; Greeley, R.; Lee, J.; Mackinnon, I.; Marshall, J.; Nickling, W.; Werner, B.; White, B.; Williams, S.

1986-01-01

The carousel wind tunnel (CWT) can be a significant tool for the determination of the nature and magnitude of interparticlar forces at threshold of motion. By altering particle and drum surface electrical properties and/or by applying electric potential difference across the inner and outer drums, it should be possible to separate electrostatic effects from other forces of cohesion. Besides particle trajectory and bedform analyses, suggestions for research include particle aggregation in zero and subgravity environments, effect of suspension-saltation ratio on soil abrasion, and the effects of shear and shearfree turbulence on particle aggregation as applied to evolution of solar nebula.

The Role of Drag Force in Shedding of Multiple Sessile Drops

NASA Astrophysics Data System (ADS)

Razzaghi, Aysan; Banitabaei, Sayyed Hossein; Amirfazli, Alidad; -Team

2017-11-01

A sessile drop placed on a solid surface can shed, if the drag force due to a shearing airflow overcomes the drop adhesion to the surface. Sessile drop shedding is of importance due to its applications in condensation, fuel cells, icing, etc. Majority of the studies so far have considered the shedding of a single sessile droplet; however, in the applications above, multiple sessile droplets appear on a surface. Shedding of sessile drops in different arrangements, i.e. tandem, side by side, triangle, and rectangle have been investigated both experimentally and through VOF simulations. The minimum air velocity (Ucr) at which the drop(s) at the upstream dislodge from the surface was measured. Drops were placed in a wind tunnel with increasing air velocity at a rate of 1m/s2. It has been found that Ucr, deviates from its value for a single drop due to presence of the neighboring drops. The amount of the deviation is closely related to the flow pattern and interaction of drop wakes which are elucidated numerically. The interacting wakes change the drag force on the drops. Generally, the adhesion force is not affected by presence of other drops. As such, when the drops' wakes are interacting strongly, Ucr can increase by 45%.

Compact forced simple-shear sample for studying shear localization in materials

DOE PAGES

Gray, George Thompson; Vecchio, K. S.; Livescu, Veronica

2015-11-06

In this paper, a new specimen geometry, the compact forced-simple-shear specimen (CFSS), has been developed as a means to achieve simple shear testing of materials over a range of temperatures and strain rates. The stress and strain state in the gage section is designed to produce essentially “pure” simple shear, mode II in-plane shear, in a compact-sample geometry. The 2-D plane of shear can be directly aligned along specified directional aspects of a material's microstructure of interest; i.e., systematic shear loading parallel, at 45°, and orthogonal to anisotropic microstructural features in a material such as the pancake-shaped grains typical inmore » many rolled structural metals, or to specified directions in fiber-reinforced composites. Finally, the shear-stress shear-strain response and the damage evolution parallel and orthogonal to the pancake grain morphology in 7039-Al are shown to vary significantly as a function of orientation to the microstructure.« less

Bioprocess Forces and Their Impact on Cell Behavior: Implications for Bone Regeneration Therapy

PubMed Central

Brindley, David; Moorthy, Kishaani; Lee, Jae-Ho; Mason, Chris; Kim, Hae-Won; Wall, Ivan

2011-01-01

Bioprocess forces such as shear stress experienced during routine cell culture are considered to be harmful to cells. However, the impact of physical forces on cell behavior is an area of growing interest within the tissue engineering community, and it is widely acknowledged that mechanical stimulation including shear stress can enhance osteogenic differentiation. This paper considers the effects of bioprocess shear stress on cell responses such as survival and proliferation in several contexts, including suspension-adapted cells used for recombinant protein and monoclonal antibody manufacture, adherent cells for therapy in suspension, and adherent cells attached to their growth substrates. The enhanced osteogenic differentiation that fluid flow shear stress is widely found to induce is discussed, along with the tissue engineering of mineralized tissue using perfusion bioreactors. Recent evidence that bioprocess forces produced during capillary transfer or pipetting of cell suspensions can enhance osteogenic responses is also discussed. PMID:21904661

Raman spectroscopy compared against traditional predictors of shear force in lamb m. longissimus lumborum.

PubMed

Fowler, Stephanie M; Schmidt, Heinar; van de Ven, Remy; Wynn, Peter; Hopkins, David L

2014-12-01

A Raman spectroscopic hand held device was used to predict shear force (SF) of 80 fresh lamb m. longissimus lumborum (LL) at 1 and 5days post mortem (PM). Traditional predictors of SF including sarcomere length (SL), particle size (PS), cooking loss (CL), percentage myofibrillar breaks and pH were also measured. SF values were regressed against Raman spectra using partial least squares regression and against the traditional predictors using linear regression. The best prediction of shear force values used spectra at 1day PM to predict shear force at 1day which gave a root mean square error of prediction (RMSEP) of 13.6 (Null=14.0) and the R(2) between observed and cross validated predicted values was 0.06 (R(2)cv). Overall, for fresh LL, the predictability SF, by either the Raman hand held probe or traditional predictors was low. Copyright © 2014 Elsevier Ltd. All rights reserved.

Approximate Stokes Drift Profiles and their use in Ocean Modelling

NASA Astrophysics Data System (ADS)

Breivik, O.; Biblot, J.; Janssen, P. A. E. M.

2016-02-01

Deep-water approximations to the Stokes drift velocity profile are explored as alternatives to the monochromatic profile. The alternative profiles investigated rely on the same two quantities required for the monochromatic profile, viz the Stokes transport and the surface Stokes drift velocity. Comparisons with parametric spectra and profiles under wave spectra from the ERA-Interim reanalysis and buoy observations reveal much better agreement than the monochromatic profile even for complex sea states. That the profiles give a closer match and a more correct shear has implications for ocean circulation models since the Coriolis-Stokes force depends on the magnitude and direction of the Stokes drift profile and Langmuir turbulence parameterizations depend sensitively on the shear of the profile. The NEMO general circulation ocean model was recently extended to incorporate the Stokes-Coriolis force along with two other wave-related effects. I will show some results from the coupled atmosphere-wave-ocean ensemble forecast system of ECMWF where these wave effects are now included in the ocean model component.

USE OF RADIOISOTOPES IN THE STUDY OF METAL-TO-METAL WEAR OF HARDENED IRON- BASE ALLOYS. Quarterly Report No. 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Talento, A.; Steven, G.

1959-04-15

A literature survey was made on the friction and wear of solid metals and on autoradiographic techniques as they apply to metal-to-metal wear studies. When two contacting surfaces are moving with respect to one another, the asperities weld together to form weld junctions. The number of junctions is large when no foreigm materials are on the contacting surfaces, but is greatly reduced by the presence of lubricants. Frictional forces are equal to the sum of the forces required to shear the weld junctions and the plough ing force. The rubbing surfaces may develop localized hot spots which may reach 2000more » F, and in these areas the metal is plastically deformed. Frictional forces and wear usually decrease as the hardness of the specimens increases. Autoradiographic techniques have been used to determine the location of radioactive tracers. Because photographic emulsions are sensitive to ionization caused by products of atomic disintegration, they are used to record the radiation given off by radioactive tracers. The wet and dry autoradiographic techniques that have been developed for metallurgical applications are described in this report. (auth)« less

Near-wall similarity in a pressure-driven three-dimensional turbulent boundary layer

NASA Technical Reports Server (NTRS)

Pierce, F. J.; Mcallister, J. E.

1980-01-01

Mean velocity, measured wall pressure and wall shear stress fields were made in a three dimensional pressure-driven turbulent boundary layer created by a cylinder with trailing edge placed normal to a flat plate floor. The direct force wall shear stress measurements were made with floating element direct force sensing shear meter that responded to both the magnitude and direction of the local wall shear stress. The ability of 10 near wall similarity models to describe the near wall velocity field for the measured flow under a wide range of skewing conditions and a variety of pressure gradient and wall shear vector orientations was used.

Influence of enamel conditioning on the shear bond strength of different adhesives.

PubMed

Brauchli, Lorenz; Muscillo, Teodoro; Steineck, Markus; Wichelhaus, Andrea

2010-11-01

Phosphoric acid etching is the gold standard for enamel conditioning. However, it is possible that air abrasion or a combination of air abrasion and etching might result in enhanced adhesion. The aim of this study was to investigate the effect of different enamel conditioning methods on the bond strength of six adhesives. Three different enamel conditioning procedures (phosphoric acid etching, air abrasion, air abrasion + phosphoric acid etching) were evaluated for their influence on the shear bond strength of six different adhesives (Transbond™ XT, Cool-Bond™, Fuji Ortho LC, Ultra Band-Lok, Tetric(®) Flow, Light-Bond™). Each group consisted of 15 specimens. Shear forces were measured with a universal testing machine. The scores of the Adhesive Remnant Index (ARI) were also analyzed. There were no significant differences between phosphoric acid etching and air abrasion + phosphoric acid etching. Air abrasion as a single conditioning technique led to significantly lower shear forces. The ARI scores did not correlate with the shear strengths measured. There were greater variations in shear forces for the different adhesives than for the conditioning techniques. The highest shear forces were found for the conventional composites Transbond™ XT and Cool- Bond™ in combination with conventional etching. Air abrasion alone and in combination with phosphoric acid etching showed no advantages compared with phosphoric acid etching alone and, therefore, cannot be recommended.

Function of the medial meniscus in force transmission and stability.

PubMed

Walker, Peter S; Arno, Sally; Bell, Christopher; Salvadore, Gaia; Borukhov, Ilya; Oh, Cheongeun

2015-06-01

We studied the combined role of the medial meniscus in distributing load and providing stability. Ten normal knees were loaded in combinations of compressive and shear loading as the knee was flexed over a full range. A digital camera tracked the motion, from which femoral-tibial contacts were determined by computer modelling. Load transmission was determined from the Tekscan for the anterior horn, central body, posterior horn, and the uncovered cartilage in the centre of the meniscus. For the three types of loading; compression only, compression and anterior shear, compression and posterior shear; between 40% and 80% of the total load was transmitted through the meniscus. The overall average was 58%, the remaining 42% being transmitted through the uncovered cartilage. The anterior horn was loaded only up to 30 degrees flexion, but played a role in controlling anterior femoral displacement. The central body was loaded 10-20% which would provide some restraint to medial femoral subluxation. Overall the posterior horn carried the highest percentage of the shear load, especially after 30 degrees flexion when a posterior shear force was applied, where the meniscus was estimated to carry 50% of the shear force. This study added new insights into meniscal function during weight bearing conditions, particularly its role in early flexion, and in transmitting shear forces. Copyright © 2015 Elsevier Ltd. All rights reserved.

Shear Model Development of Limestone Joints with Incorporating Variations of Basic Friction Coefficient and Roughness Components During Shearing

NASA Astrophysics Data System (ADS)

Mehrishal, Seyedahmad; Sharifzadeh, Mostafa; Shahriar, Korosh; Song, Jae-Jon

2017-04-01

In relation to the shearing of rock joints, the precise and continuous evaluation of asperity interlocking, dilation, and basic friction properties has been the most important task in the modeling of shear strength. In this paper, in order to investigate these controlling factors, two types of limestone joint samples were prepared and CNL direct shear tests were performed on these joints under various shear conditions. One set of samples were travertine and another were onyx marble with slickensided surfaces, surfaces ground to #80, and rough surfaces were tested. Direct shear experiments conducted on slickensided and ground surfaces of limestone indicated that by increasing the applied normal stress, under different shearing rates, the basic friction coefficient decreased. Moreover, in the shear tests under constant normal stress and shearing rate, the basic friction coefficient remained constant for the different contact sizes. The second series of direct shear experiments in this research was conducted on tension joint samples to evaluate the effect of surface roughness on the shear behavior of the rough joints. This paper deals with the dilation and roughness interlocking using a method that characterizes the surface roughness of the joint based on a fundamental combined surface roughness concept. The application of stress-dependent basic friction and quantitative roughness parameters in the continuous modeling of the shear behavior of rock joints is an important aspect of this research.

Quantification of the Effect of Cross-shear on the Wear of Conventional and Highly Cross-linked UHMWPE

PubMed Central

Kang, Lu; Galvin, Alison L.; Brown, Thomas D.; Jin, Zhongmin; Fisher, John

2008-01-01

A computational model has been developed to quantify the degree of cross-shear of a polyethylene pin articulating against a metallic plate, based on the direct simulation of a multidirectional pin-on-plate wear machine. The principal molecular orientation (PMO) was determined for each polymer site. The frictional work in the direction perpendicular to the PMO was assumed to produce the greatest orientation softening (Wang et al., 1997). The cross-shear ratio (CS) was defined as the frictional work perpendicular to the PMO direction, divided by the total frictional work. Cross-shear on the pin contact surface was location-specific, and of continuously changing magnitude because the direction of frictional force continuously changed due to pin rotation. The polymer pin motion was varied from a purely linear track (CS=0) up to a maximum rotation of ±55° (CS=0.254). The relationship between wear factors (K) measured experimentally and theoretically predicted CS was defined using logarithmic functions for both conventional and highly cross-linked UHMWPE. Cross-shear increased the apparent wear factor for both polyethylenes by more than 5-fold compared to unidirectional wear. PMID:17936763

Drag reduction using wrinkled surfaces in high Reynolds number laminar boundary layer flows

NASA Astrophysics Data System (ADS)

Raayai-Ardakani, Shabnam; McKinley, Gareth H.

2017-09-01

Inspired by the design of the ribbed structure of shark skin, passive drag reduction methods using stream-wise riblet surfaces have previously been developed and tested over a wide range of flow conditions. Such textures aligned in the flow direction have been shown to be able to reduce skin friction drag by 4%-8%. Here, we explore the effects of periodic sinusoidal riblet surfaces aligned in the flow direction (also known as a "wrinkled" texture) on the evolution of a laminar boundary layer flow. Using numerical analysis with the open source Computational Fluid Dynamics solver OpenFOAM, boundary layer flow over sinusoidal wrinkled plates with a range of wavelength to plate length ratios ( λ / L ), aspect ratios ( 2 A / λ ), and inlet velocities are examined. It is shown that in the laminar boundary layer regime, the riblets are able to retard the viscous flow inside the grooves creating a cushion of stagnant fluid that the high-speed fluid above can partially slide over, thus reducing the shear stress inside the grooves and the total integrated viscous drag force on the plate. Additionally, we explore how the boundary layer thickness, local average shear stress distribution, and total drag force on the wrinkled plate vary with the aspect ratio of the riblets as well as the length of the plate. We show that riblets with an aspect ratio of close to unity lead to the highest reduction in the total drag, and that because of the interplay between the local stress distribution on the plate and stream-wise evolution of the boundary layer the plate has to exceed a critical length to give a net decrease in the total drag force.

Coupling with ocean mixed layer leads to intraseasonal variability in tropical deep convection: Evidence from cloud-resolving simulations

NASA Astrophysics Data System (ADS)

Anber, Usama; Wang, Shuguang; Sobel, Adam

2017-03-01

The effect of coupling a slab ocean mixed layer to atmospheric convection is examined in cloud-resolving model (CRM) simulations in vertically sheared and unsheared environments without Coriolis force, with the large-scale circulation parameterized using the Weak Temperature Gradient (WTG) approximation. Surface fluxes of heat and moisture as well as radiative fluxes are fully interactive, and the vertical profile of domain-averaged horizontal wind is strongly relaxed toward specified profiles with vertical shear that varies from one simulation to the next. Vertical wind shear is found to play a critical role in the simulated behavior. There exists a threshold value of the shear strength above which the coupled system develops regular oscillations between deep convection and dry nonprecipitating states, similar to those found earlier in a much more idealized model which did not consider wind shear. The threshold value of the vertical shear found here varies with the depth of the ocean mixed layer. The time scale of the spontaneously generated oscillations also varies with mixed layer depth, from 10 days with a 1 m deep mixed layer to 50 days with a 10 m deep mixed layer. The results suggest the importance of the interplay between convection organized by vertical wind shear, radiative feedbacks, large-scale dynamics, and ocean mixed layer heat storage in real intraseasonal oscillations.

Estimation of viscoelastic parameters in Prony series from shear wave propagation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jung, Jae-Wook; Hong, Jung-Wuk, E-mail: j.hong@kaist.ac.kr, E-mail: jwhong@alum.mit.edu; Lee, Hyoung-Ki

2016-06-21

When acquiring accurate ultrasonic images, we must precisely estimate the mechanical properties of the soft tissue. This study investigates and estimates the viscoelastic properties of the tissue by analyzing shear waves generated through an acoustic radiation force. The shear waves are sourced from a localized pushing force acting for a certain duration, and the generated waves travel horizontally. The wave velocities depend on the mechanical properties of the tissue such as the shear modulus and viscoelastic properties; therefore, we can inversely calculate the properties of the tissue through parametric studies.

On the Possible Role of Gently Sloping Topography in Influencing the Nature of Convective Storms: A Case Study on the High Plains of the U. S.

NASA Astrophysics Data System (ADS)

Bluestein, H. B.; Weiss, C.; Rotunno, R.; Reif, D. W.; Romine, G. S.

2016-12-01

On 27 May 2015 a quasi-stationary supercell in the northern Texas Panhandle produced several tornadoes, before it evolved into a mesoscale convective system. The pre-storm environment was characterized by relatively weak midlevel winds from the west and surface winds from the south/southwest, such that the 0 - 6 km vertical shear was too weak for supercells to have evolved. There was, however, an increase in speed and backing of the surface wind to the easterly/southeasterly direction during the afternoon, so that the 0 - 6 km shear exceeded 20 m s-1, the approximate threshold for supercell formation. The approximate motion of a convective storm without taking into account shear-related or gust-front propagation is the mean wind in the lowest 6 km. With an increase in the easterly component of the surface winds, the mean wind in the lowest 6 km, in the absence of any increase in westerlies at 6 km, decreased. Some physical mechanisms that could be responsible for the backing and increase in easterly component of the surface wind are as follows: (a) the approach of a synoptic-scale, upper-level trough, with its attendant pre-trough, quasi-geostrophic-induced ascent accompanied by surface convergence, an increase in surface vorticity, and concomitant drop in surface pressure, so that east of the region of ascent a westward-directed pressure-gradient forced develops; (b) surface heating on a surface that slopes upward to the west, such that a westward-directed pressure-gradient force develops; (c) the westward movement of a low-level cold pool that had developed in pre-existing, upstream convective storms; (d) the downward mixing of easterly momentum aloft; and (e) the diurnal inertial oscillation in boundary-layer wind due to the diurnal change in vertical mixing of momentum. It is hypothesized that for this case the primary mechanism was (b). A WRF forecast is described in which evidence is presented in favor of our hypothesis. An estimate will also be given of how much backing and increase in surface wind could be explained by heating on the sloping terrain and also climatological changes in wind speed and direction during the afternoon based on surface mesonet data will be shown. The results of this study could have applications to the study of convection anywhere around the globe where there is gently sloping terrain.

Diffusion of microspheres in shear flow near a wall: use to measure binding rates between attached molecules.

PubMed Central

Pierres, A; Benoliel, A M; Zhu, C; Bongrand, P

2001-01-01

The rate and distance-dependence of association between surface-attached molecules may be determined by monitoring the motion of receptor-bearing spheres along ligand-coated surfaces in a flow chamber (Pierres et al., Proc. Natl. Acad. Sci. U.S.A. 95:9256-9261, 1998). Particle arrests reveal bond formation, and the particle-to-surface distance may be estimated from the ratio between the velocity and the wall shear rate. However, several problems are raised. First, data interpretation requires extensive computer simulations. Second, the relevance of standard results from fluid mechanics to micrometer-size particles separated from surfaces by nanometer distances is not fully demonstrated. Third, the wall shear rate must be known with high accuracy. Here we present a simple derivation of an algorithm permitting one to simulate the motion of spheres near a plane in shear flow. We check that theoretical predictions are consistent with the experimental dependence of motion on medium viscosity or particle size, and the requirement for equilibrium particle height distribution to follow Boltzman's law. The determination of the statistical relationship between particle velocity and acceleration allows one to derive the wall shear rate with 1-s(-1) accuracy and the Hamaker constant of interaction between the particle and the wall with a sensitivity better than 10(-21) J. It is demonstrated that the correlation between particle height and mean velocity during a time interval Deltat is maximal when Deltat is about 0.1-0.2 s for a particle of 1.4-microm radius. When the particle-to-surface distance ranges between 10 and 40 nm, the particle height distribution may be obtained with a standard deviation ranging between 8 and 25 nm, provided the average velocity during a 160-ms period of time is determined with 10% accuracy. It is concluded that the flow chamber allows one to detect the formation of individual bonds with a minimal lifetime of 40 ms in presence of a disruptive force of approximately 5 pN and to assess the distance dependence within the tens of nanometer range. PMID:11423392

Bioinspired superhydrophobic surfaces, fabricated through simple and scalable roll-to-roll processing

PubMed Central

Park, Sung-Hoon; Lee, Sangeui; Moreira, David; Bandaru, Prabhakar R.; Han, InTaek; Yun, Dong-Jin

2015-01-01

A simple, scalable, non-lithographic, technique for fabricating durable superhydrophobic (SH) surfaces, based on the fingering instabilities associated with non-Newtonian flow and shear tearing, has been developed. The high viscosity of the nanotube/elastomer paste has been exploited for the fabrication. The fabricated SH surfaces had the appearance of bristled shark skin and were robust with respect to mechanical forces. While flow instability is regarded as adverse to roll-coating processes for fabricating uniform films, we especially use the effect to create the SH surface. Along with their durability and self-cleaning capabilities, we have demonstrated drag reduction effects of the fabricated films through dynamic flow measurements. PMID:26490133

Bioinspired superhydrophobic surfaces, fabricated through simple and scalable roll-to-roll processing.

PubMed

Park, Sung-Hoon; Lee, Sangeui; Moreira, David; Bandaru, Prabhakar R; Han, InTaek; Yun, Dong-Jin

2015-10-22

A simple, scalable, non-lithographic, technique for fabricating durable superhydrophobic (SH) surfaces, based on the fingering instabilities associated with non-Newtonian flow and shear tearing, has been developed. The high viscosity of the nanotube/elastomer paste has been exploited for the fabrication. The fabricated SH surfaces had the appearance of bristled shark skin and were robust with respect to mechanical forces. While flow instability is regarded as adverse to roll-coating processes for fabricating uniform films, we especially use the effect to create the SH surface. Along with their durability and self-cleaning capabilities, we have demonstrated drag reduction effects of the fabricated films through dynamic flow measurements.

Effects of Roughness and Inertia on Precursors to Frictional Sliding

NASA Astrophysics Data System (ADS)

Robbins, Mark O.; Salerno, K. Michael

2012-02-01

Experiments show that when a PMMA block on a surface is normally loaded and driven by an external shear force, contact at the interface is modified in discrete precursor slips prior to steady state sliding.[1] Our simulations use an atomistic model of a rough two-dimensional block in contact with a flat surface to investigate the evolution of stress and displacement along the contact between surfaces. The talk will show how local and global stress conditions govern the initiation of interfacial cracks as well as the spatial extension of the cracked region. Inertia also plays an important role in determining the number and size of slips before sliding and influences the distribution of stresses at the interface. Finally, the geometry of surface asperities also influences the interfacial evolution and the total friction force. The relationship between the interfacial stress state and rupture velocity will also be discussed. [1] S.M. Rubinstein, G. Cohen and J. Fineberg, PRL 98, 226103 (2007)

Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing.

PubMed

Thomen, Philippe; Robert, Jérôme; Monmeyran, Amaury; Bitbol, Anne-Florence; Douarche, Carine; Henry, Nelly

2017-01-01

Bacterial communities attached to surfaces under fluid flow represent a widespread lifestyle of the microbial world. Through shear stress generation and molecular transport regulation, hydrodynamics conveys effects that are very different by nature but strongly coupled. To decipher the influence of these levers on bacterial biofilms immersed in moving fluids, we quantitatively and simultaneously investigated physicochemical and biological properties of the biofilm. We designed a millifluidic setup allowing to control hydrodynamic conditions and to monitor biofilm development in real time using microscope imaging. We also conducted a transcriptomic analysis to detect a potential physiological response to hydrodynamics. We discovered that a threshold value of shear stress determined biofilm settlement, with sub-piconewton forces sufficient to prevent biofilm initiation. As a consequence, distinct hydrodynamic conditions, which set spatial distribution of shear stress, promoted distinct colonization patterns with consequences on the growth mode. However, no direct impact of mechanical forces on biofilm growth rate was observed. Consistently, no mechanosensing gene emerged from our differential transcriptomic analysis comparing distinct hydrodynamic conditions. Instead, we found that hydrodynamic molecular transport crucially impacts biofilm growth by controlling oxygen availability. Our results shed light on biofilm response to hydrodynamics and open new avenues to achieve informed design of fluidic setups for investigating, engineering or fighting adherent communities.

Measurement of Mechanical Properties of Soft Tissue with Ultrasound Vibrometry

NASA Astrophysics Data System (ADS)

Nenadich, I.; Bernal, M.; Greenleaf, J. F.

The cardiovascular diseases atherosclerosis, coronary artery disease, hypertension and heart failure have been related to stiffening of vessels and myocardium. Noninvasive measurements of mechanical properties of cardiovascular tissue would facilitate detection and treatment of disease in early stages, thus reducing mortality and possibly reducing cost of treatment. While techniques capable of measuring tissue elasticity have been reported, the knowledge of both elasticity and viscosity is necessary to fully characterize mechanical properties of soft tissues. In this article, we summarize the Shearwave Dispersion Ultrasound Vibrometry (SDUV) method developed by our group and report on advances made in characterizing stiffness of large vessels and myocardium. The method uses radiation forceFadiation force to excite shear waves in soft tissue and pulse echo ultrasound to measure the motion. The speed of propagation of shear waves at different frequencies is used to generate dispersions curves for excised porcine left-ventricular free-wall myocardium and carotid arteries. An antisymmetric Lamb wave model was fitted to the LV myocardium dispersion curves to obtain elasticity and viscosity moduli. The results suggest that the speed of shear wave propagation in four orthogonal directions on the surface of the excised myocardium is similar. These studies show that the SDUV method has potential for clinical application in noninvasive quantification of elasticity and viscosity of vessels and myocardium.

Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing

PubMed Central

Thomen, Philippe; Robert, Jérôme; Monmeyran, Amaury; Bitbol, Anne-Florence; Douarche, Carine; Henry, Nelly

2017-01-01

Bacterial communities attached to surfaces under fluid flow represent a widespread lifestyle of the microbial world. Through shear stress generation and molecular transport regulation, hydrodynamics conveys effects that are very different by nature but strongly coupled. To decipher the influence of these levers on bacterial biofilms immersed in moving fluids, we quantitatively and simultaneously investigated physicochemical and biological properties of the biofilm. We designed a millifluidic setup allowing to control hydrodynamic conditions and to monitor biofilm development in real time using microscope imaging. We also conducted a transcriptomic analysis to detect a potential physiological response to hydrodynamics. We discovered that a threshold value of shear stress determined biofilm settlement, with sub-piconewton forces sufficient to prevent biofilm initiation. As a consequence, distinct hydrodynamic conditions, which set spatial distribution of shear stress, promoted distinct colonization patterns with consequences on the growth mode. However, no direct impact of mechanical forces on biofilm growth rate was observed. Consistently, no mechanosensing gene emerged from our differential transcriptomic analysis comparing distinct hydrodynamic conditions. Instead, we found that hydrodynamic molecular transport crucially impacts biofilm growth by controlling oxygen availability. Our results shed light on biofilm response to hydrodynamics and open new avenues to achieve informed design of fluidic setups for investigating, engineering or fighting adherent communities. PMID:28403171

Computational analysis of microbubble flows in bifurcating airways: role of gravity, inertia, and surface tension.

PubMed

Chen, Xiaodong; Zielinski, Rachel; Ghadiali, Samir N

2014-10-01

Although mechanical ventilation is a life-saving therapy for patients with severe lung disorders, the microbubble flows generated during ventilation generate hydrodynamic stresses, including pressure and shear stress gradients, which damage the pulmonary epithelium. In this study, we used computational fluid dynamics to investigate how gravity, inertia, and surface tension influence both microbubble flow patterns in bifurcating airways and the magnitude/distribution of hydrodynamic stresses on the airway wall. Direct interface tracking and finite element techniques were used to simulate bubble propagation in a two-dimensional (2D) liquid-filled bifurcating airway. Computational solutions of the full incompressible Navier-Stokes equation were used to investigate how inertia, gravity, and surface tension forces as characterized by the Reynolds (Re), Bond (Bo), and Capillary (Ca) numbers influence pressure and shear stress gradients at the airway wall. Gravity had a significant impact on flow patterns and hydrodynamic stress magnitudes where Bo > 1 led to dramatic changes in bubble shape and increased pressure and shear stress gradients in the upper daughter airway. Interestingly, increased pressure gradients near the bifurcation point (i.e., carina) were only elevated during asymmetric bubble splitting. Although changes in pressure gradient magnitudes were generally more sensitive to Ca, under large Re conditions, both Re and Ca significantly altered the pressure gradient magnitude. We conclude that inertia, gravity, and surface tension can all have a significant impact on microbubble flow patterns and hydrodynamic stresses in bifurcating airways.

In-shoe plantar tri-axial stress profiles during maximum-effort cutting maneuvers.

PubMed

Cong, Yan; Lam, Wing Kai; Cheung, Jason Tak-Man; Zhang, Ming

2014-12-18

Soft tissue injuries, such as anterior cruciate ligament rupture, ankle sprain and foot skin problems, frequently occur during cutting maneuvers. These injuries are often regarded as associated with abnormal joint torque and interfacial friction caused by excessive external and in-shoe shear forces. This study simultaneously investigated the dynamic in-shoe localized plantar pressure and shear stress during lateral shuffling and 45° sidestep cutting maneuvers. Tri-axial force transducers were affixed at the first and second metatarsal heads, lateral forefoot, and heel regions in the midsole of a basketball shoe. Seventeen basketball players executed both cutting maneuvers with maximum efforts. Lateral shuffling cutting had a larger mediolateral braking force than 45° sidestep cutting. This large braking force was concentrated at the first metatarsal head, as indicated by its maximum medial shear stress (312.2 ± 157.0 kPa). During propulsion phase, peak shear stress occurred at the second metatarsal head (271.3 ± 124.3 kPa). Compared with lateral shuffling cutting, 45° sidestep cutting produced larger peak propulsion shear stress (463.0 ± 272.6 kPa) but smaller peak braking shear stress (184.8 ± 181.7 kPa), of which both were found at the first metatarsal head. During both cutting maneuvers, maximum medial and posterior shear stress occurred at the first metatarsal head, whereas maximum pressure occurred at the second metatarsal head. The first and second metatarsal heads sustained relatively high pressure and shear stress and were expected to be susceptible to plantar tissue discomfort or injury. Due to different stress distribution, distinct pressure and shear cushioning mechanisms in basketball footwear might be considered over different foot regions. Copyright © 2014 Elsevier Ltd. All rights reserved.

Swelling-Induced Folding in Confined Nanoscale Responsive Polymer Gels

DTIC Science & Technology

2010-03-16

transformations leading to micrometer scale lenticular surface structures due to strong shear forces at the filmsubstrate interface. The growth of the...observed here. To further understand the origin of the observed lenticular folding patterns, we considered how the con- ditions for buckling patterns in...periodic- ity of 900 nm) exhibited organized lenticular structures popping up from nanoimprinted film similar to that ob- served in a uniform flat

Localization of adhesins on the surface of a pathogenic bacterial envelope through atomic force microscopy

NASA Astrophysics Data System (ADS)

Arnal, L.; Longo, G.; Stupar, P.; Castez, M. F.; Cattelan, N.; Salvarezza, R. C.; Yantorno, O. M.; Kasas, S.; Vela, M. E.

2015-10-01

Bacterial adhesion is the first and a significant step in establishing infection. This adhesion normally occurs in the presence of flow of fluids. Therefore, bacterial adhesins must be able to provide high strength interactions with their target surface in order to maintain the adhered bacteria under hydromechanical stressing conditions. In the case of B. pertussis, a Gram-negative bacterium responsible for pertussis, a highly contagious human respiratory tract infection, an important protein participating in the adhesion process is a 220 kDa adhesin named filamentous haemagglutinin (FHA), an outer membrane and also secreted protein that contains recognition domains to adhere to ciliated respiratory epithelial cells and macrophages. In this work, we obtained information on the cell-surface localization and distribution of the B. pertussis adhesin FHA using an antibody-functionalized AFM tip. Through the analysis of specific molecular recognition events we built a map of the spatial distribution of the adhesin which revealed a non-homogeneous pattern. Moreover, our experiments showed a force induced reorganization of the adhesin on the surface of the cells, which could explain a reinforced adhesive response under external forces. This single-molecule information contributes to the understanding of basic molecular mechanisms used by bacterial pathogens to cause infectious disease and to gain insights into the structural features by which adhesins can act as force sensors under mechanical shear conditions.Bacterial adhesion is the first and a significant step in establishing infection. This adhesion normally occurs in the presence of flow of fluids. Therefore, bacterial adhesins must be able to provide high strength interactions with their target surface in order to maintain the adhered bacteria under hydromechanical stressing conditions. In the case of B. pertussis, a Gram-negative bacterium responsible for pertussis, a highly contagious human respiratory tract infection, an important protein participating in the adhesion process is a 220 kDa adhesin named filamentous haemagglutinin (FHA), an outer membrane and also secreted protein that contains recognition domains to adhere to ciliated respiratory epithelial cells and macrophages. In this work, we obtained information on the cell-surface localization and distribution of the B. pertussis adhesin FHA using an antibody-functionalized AFM tip. Through the analysis of specific molecular recognition events we built a map of the spatial distribution of the adhesin which revealed a non-homogeneous pattern. Moreover, our experiments showed a force induced reorganization of the adhesin on the surface of the cells, which could explain a reinforced adhesive response under external forces. This single-molecule information contributes to the understanding of basic molecular mechanisms used by bacterial pathogens to cause infectious disease and to gain insights into the structural features by which adhesins can act as force sensors under mechanical shear conditions. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr04644k

Rheotaxis of Bimetallic Micromotors Driven by Chemical-Acoustic Hybrid Power.

PubMed

Ren, Liqiang; Zhou, Dekai; Mao, Zhangming; Xu, Pengtao; Huang, Tony Jun; Mallouk, Thomas E

2017-10-24

Rheotaxis is a common phenomenon in nature that refers to the directed movement of micro-organisms as a result of shear flow. The ability to mimic natural rheotaxis using synthetic micro/nanomotors adds functionality to enable their applications in biomedicine and chemistry. Here, we present a hybrid strategy that can achieve both positive and negative rheotaxis of synthetic bimetallic micromotors by employing a combination of chemical fuel and acoustic force. An acoustofluidic device is developed for the integration of the two propulsion mechanisms. Using acoustic force alone, bimetallic microrods are propelled along the bottom surface in the center of a fluid channel. The leading end of the microrod is always the less dense end, as established in earlier experiments. With chemical fuel (H 2 O 2 ) alone, the microrods orient themselves with their anode end against the flow when shear flow is present. Numerical simulations confirm that this orientation results from tilting of the microrods relative to the bottom surface of the channel, which is caused by catalytically driven electro-osmotic flow. By combining this catalytic orientation effect with more powerful, density-dependent acoustic propulsion, both positive and negative rheotaxis can be achieved. The ability to respond to flow stimuli and collectively propel synthetic microswimmers in a directed manner indicates an important step toward practical applications.

Electromotive force and large-scale magnetic dynamo in a turbulent flow with a mean shear.

PubMed

Rogachevskii, Igor; Kleeorin, Nathan

2003-09-01

An effect of sheared large-scale motions on a mean electromotive force in a nonrotating turbulent flow of a conducting fluid is studied. It is demonstrated that in a homogeneous divergence-free turbulent flow the alpha effect does not exist, however a mean magnetic field can be generated even in a nonrotating turbulence with an imposed mean velocity shear due to a "shear-current" effect. A mean velocity shear results in an anisotropy of turbulent magnetic diffusion. A contribution to the electromotive force related to the symmetric parts of the gradient tensor of the mean magnetic field (the kappa effect) is found in nonrotating turbulent flows with a mean shear. The kappa effect and turbulent magnetic diffusion reduce the growth rate of the mean magnetic field. It is shown that a mean magnetic field can be generated when the exponent of the energy spectrum of the background turbulence (without the mean velocity shear) is less than 2. The shear-current effect was studied using two different methods: the tau approximation (the Orszag third-order closure procedure) and the stochastic calculus (the path integral representation of the solution of the induction equation, Feynman-Kac formula, and Cameron-Martin-Girsanov theorem). Astrophysical applications of the obtained results are discussed.

Shear-induced aggregation or disaggregation in edible oils: Models, computer simulation, and USAXS measurements

NASA Astrophysics Data System (ADS)

Townsend, B.; Peyronel, F.; Callaghan-Patrachar, N.; Quinn, B.; Marangoni, A. G.; Pink, D. A.

2017-12-01

The effects of shear upon the aggregation of solid objects formed from solid triacylglycerols (TAGs) immersed in liquid TAG oils were modeled using Dissipative Particle Dynamics (DPD) and the predictions compared to experimental data using Ultra-Small Angle X-ray Scattering (USAXS). The solid components were represented by spheres interacting via attractive van der Waals forces and short range repulsive forces. A velocity was applied to the liquid particles nearest to the boundary, and Lees-Edwards boundary conditions were used to transmit this motion to non-boundary layers via dissipative interactions. The shear was created through the dissipative forces acting between liquid particles. Translational diffusion was simulated, and the Stokes-Einstein equation was used to relate DPD length and time scales to SI units for comparison with USAXS results. The SI values depended on how large the spherical particles were (250 nm vs. 25 nm). Aggregation was studied by (a) computing the Structure Function and (b) quantifying the number of pairs of solid spheres formed. Solid aggregation was found to be enhanced by low shear rates. As the shear rate was increased, a transition shear region was manifested in which aggregation was inhibited and shear banding was observed. Aggregation was inhibited, and eventually eliminated, by further increases in the shear rate. The magnitude of the transition region shear, γ˙ t, depended on the size of the solid particles, which was confirmed experimentally.

Different knee joint loading patterns in ACL deficient copers and non-copers during walking.

PubMed

Alkjær, Tine; Henriksen, Marius; Simonsen, Erik B

2011-04-01

Rupture of the anterior cruciate ligament (ACL) causes changes in the walking pattern. ACL deficient subjects classified as copers and non-copers have been observed to adopt different post-injury walking patterns. How these different patterns affect the knee compression and shear forces is unresolved. Thus, the aim of the present study was to investigate how different walking patterns observed between copers, non-copers, and controls affect the knee compression and shear forces during walking. Three-dimensional gait analyses were performed in copers (n = 9), non-copers (n = 10), and control subjects (n =19). The net knee joint moment, knee joint reaction forces, and the sagittal knee joint angle were input parameters to a biomechanical model that assessed the knee compression and shear forces. The results showed that the non-copers walked with significantly reduced knee compression and shear forces than the controls. The overall knee compression force pattern was similar between the copers and controls, although this variable was significantly increased at heel strike in the copers compared to both non-copers and controls. The peak shear force was significantly dependent on the peak knee extensor moment. This covariance was significantly different between groups meaning that at a given knee extensor moment the shear force was significantly reduced in the copers compared to controls. The different knee joint loading patterns observed between non-copers and copers reflected the different walking strategies adopted by these groups, which may have implications for the knee joint stability. The strategy adopted by the copers may resemble an effective way to stabilize the knee joint during walking after an ACL rupture and that the knee kinematics may play a key role for this strategy. It is clinically relevant to investigate if gait retraining would enable non-copers to walk as copers and thereby improve their knee joint stability.

Do rotational shear-cushioning shoes influence horizontal ground reaction forces and perceived comfort during basketball cutting maneuvers?

PubMed

Lam, Wing-Kai; Qu, Yi; Yang, Fan; Cheung, Roy T H

2017-01-01

Court shoe designs predominantly focus on reducing excessive vertical ground reaction force, but shear force cushioning has received little attention in the basketball population. We aimed to examine the effect of a novel shoe-cushioning design on both resultant horizontal ground reaction forces and comfort perception during two basketball-specific cutting movements. Fifteen university team basketball players performed lateral shuffling and 45-degree sidestep cutting at maximum effort in basketball shoes with and without the shear-cushioning system (SCS). Paired t -tests were used to examine the differences in kinetics and comfort perception between two shoes. SCS shoe allowed for larger rotational material deformation compared with control shoes, but no significant shoe differences were found in braking phase kinetics during both cutting movements ( P  = 0.35). Interestingly, a greater horizontal propulsion impulse was found with the SCS during 45-degree cutting ( P  < 0.05), when compared with the control. In addition, players wearing SCS shoes perceived better forefoot comfort ( P  = 0.012). During lateral shuffling, there were no significant differences in horizontal GRF and comfort perception between shoe conditions ( P  > 0.05). The application of a rotational shear-cushioning structure allowed for better forefoot comfort and enhanced propulsion performance in cutting, but did not influence the shear impact. Understanding horizontal ground reaction force information may be useful in designing footwear to prevent shear-related injuries in sport populations.

Quantitative Percussion Diagnostics For Evaluating Bond Integrity Between Composite Laminates

NASA Astrophysics Data System (ADS)

Poveromo, Scott Leonard

Conventional nondestructive testing (NDT) techniques used to detect defects in composites are not able to determine intact bond integrity within a composite structure and are costly to use on large and complex shaped surfaces. To overcome current NDT limitations, a new technology was utilized based on quantitative percussion diagnostics (QPD) to better quantify bond quality in fiber reinforced composite materials. Experimental results indicate that this technology is capable of detecting 'kiss' bonds (very low adhesive shear strength), caused by the application of release agents on the bonding surfaces, between flat composite laminates bonded together with epoxy adhesive. Specifically, the local value of the loss coefficient determined from quantitative percussion testing was found to be significantly greater for a release coated panel compared to that for a well bonded sample. Also, the local value of the probe force or force returned to the probe after impact was observed to be lower for the release coated panels. The increase in loss coefficient and decrease in probe force are thought to be due to greater internal friction during the percussion event for poorly bonded specimens. NDT standards were also fabricated by varying the cure parameters of an epoxy film adhesive. Results from QPD for the variable cure NDT standards and lap shear strength measurements taken of mechanical test specimens were compared and analyzed. Finally, experimental results have been compared to a finite element analysis to understand the visco-elastic behavior of the laminates during percussion testing. This comparison shows how a lower quality bond leads to a reduction in the percussion force by biasing strain in the percussion tested side of the panel.

Mechanical interaction between neighboring muscles in human upper limb: Evidence for epimuscular myofascial force transmission in humans.

PubMed

Yoshitake, Yasuhide; Uchida, Daiki; Hirata, Kosuke; Mayfield, Dean L; Kanehisa, Hiroaki

2018-06-06

To confirm the existence of epimuscular myofascial force transmission in humans, this study examined if manipulating joint angle to stretch the muscle can alter the shear modulus of a resting adjacent muscle, and whether there are regional differences in this response. The biceps brachii (BB: manipulated muscle) and the brachialis (BRA: resting adjacent muscle) were deemed suitable for this study because they are neighboring, yet have independent tendons that insert onto different bones. In order to manipulate the muscle length of BB only, the forearm was passively set at supination, neutral, and pronation positions. For thirteen healthy young adult men, the shear modulus of BB and BRA was measured with shear-wave elastography at proximal and distal muscle regions for each forearm position and with the elbow joint angle at either 100° or 160°. At both muscle regions and both elbow positions, BB shear modulus increased as the forearm was rotated from a supinated to pronated position. Conversely, BRA shear modulus decreased as function of forearm position. The effect of forearm position on shear modulus was most pronounced in the distal muscle region when the elbow was at 160°. The observed alteration of shear modulus of the resting adjacent muscle indicates that epimuscular myofascial force transmission is present in the human upper limb. Consistent with this assertion, we found that the effect of muscle length on shear modulus in both muscles was region-dependent. Our results also suggest that epimuscular myofascial force transmission may be facilitated at stretched muscle lengths. Copyright © 2018 Elsevier Ltd. All rights reserved.

Surface shear inviscidity of soluble surfactants

PubMed Central

Zell, Zachary A.; Nowbahar, Arash; Mansard, Vincent; Leal, L. Gary; Deshmukh, Suraj S.; Mecca, Jodi M.; Tucker, Christopher J.; Squires, Todd M.

2014-01-01

Foam and emulsion stability has long been believed to correlate with the surface shear viscosity of the surfactant used to stabilize them. Many subtleties arise in interpreting surface shear viscosity measurements, however, and correlations do not necessarily indicate causation. Using a sensitive technique designed to excite purely surface shear deformations, we make the most sensitive and precise measurements to date of the surface shear viscosity of a variety of soluble surfactants, focusing on SDS in particular. Our measurements reveal the surface shear viscosity of SDS to be below the sensitivity limit of our technique, giving an upper bound of order 0.01 μN·s/m. This conflicts directly with almost all previous studies, which reported values up to 103–104 times higher. Multiple control and complementary measurements confirm this result, including direct visualization of monolayer deformation, for SDS and a wide variety of soluble polymeric, ionic, and nonionic surfactants of high- and low-foaming character. No soluble, small-molecule surfactant was found to have a measurable surface shear viscosity, which seriously undermines most support for any correlation between foam stability and surface shear rheology of soluble surfactants. PMID:24563383

Ballooning instabilities in tokamaks with sheared toroidal flows

DOE Office of Scientific and Technical Information (OSTI.GOV)

Waelbroeck, F.L.; Chen, L.

1990-11-01

The stability of ballooning modes in the presence of sheared toroidal flows is investigated. The eigenmodes are shown to be related by a Fourier transformation to the non-exponentially growing Floquet solutions found by Cooper. It is further shown that the problem cannot be reduced further than to a two dimensional partial differential equation. Next, the generalized ballooning equation is solved analytically for a circular tokamak equilibrium with sonic flows, but with a small rotation shear compared to the sound speed. With this ordering, the centrifugal forces are comparable to the pressure gradient forces driving the instability, but coupling of themore » mode with the sound wave is avoided. A new stability criterion is derived which explicitly demonstrates that flow shear is stabilizing at constant centrifugal force gradient. 34 refs.« less

Imaging the microscopic structure of shear thinning and thickening colloidal suspensions.

PubMed

Cheng, Xiang; McCoy, Jonathan H; Israelachvili, Jacob N; Cohen, Itai

2011-09-02

The viscosity of colloidal suspensions varies with shear rate, an important effect encountered in many natural and industrial processes. Although this non-Newtonian behavior is believed to arise from the arrangement of suspended particles and their mutual interactions, microscopic particle dynamics are difficult to measure. By combining fast confocal microscopy with simultaneous force measurements, we systematically investigate a suspension's structure as it transitions through regimes of different flow signatures. Our measurements of the microscopic single-particle dynamics show that shear thinning results from the decreased relative contribution of entropic forces and that shear thickening arises from particle clustering induced by hydrodynamic lubrication forces. This combination of techniques illustrates an approach that complements current methods for determining the microscopic origins of non-Newtonian flow behavior in complex fluids.

Comparison of the shear bond strength of self-adhesive resin cements to enamel and dentin with different protocol of application

PubMed Central

Moghaddas, Mohammad Javad; Hossainipour, Zahra; Majidinia, Sara; Ojrati, Najmeh

2017-01-01

Aim The aim of the present study was to determine the shear bond strength of self-adhesive resin cements to enamel and dentin with and without surface treatments, and compare them with conventional resin cement as the control group. Methods In this experimental study, buccal and lingual surface of the thirty sound human premolars were polished in order to obtain a flat surface of enamel (E) in buccal, and dentin (D) in lingual. Sixty feldspathic ceramic blocks (2×3×3 mm) were prepared and randomly divided into six groups (n=10). Each block was cemented to the prepared surface (30 enamel and 30 dentin surface) according to different protocol: E1 and D1; RelyX ARC as control group, E2, D2; RelyX Unicem, E3, D3; acid etching +RelyX Unicem. The specimens were termocycled and subjected to shear forces by a universal testing machine at a cross head speed of 0.5 mm/min. The mode of fracture were evaluated by stereomicroscope. Data were analyzed with descriptive statistical methods using SPSS version 15. One-way ANOVA, and post hoc Tukey tests were used to compare bond strengths between the groups with different adhesives at α=0.05. Results Statistical analysis showed no significant differences within the enamel subgroups, but there were significant differences within the dentinal subgroups, and statistically significant differences were found between the groups D1and D3 (p=0.02). Comparison between similar enamel and dentinal subgroups showed that there was a significant difference just between the subgroups E3 and D3 (p=0.01). Conclusion Elective etching of enamel did not lead to significant increase in the shear bond strength of RelyX Unicem in comparison to RelyX ARC. On the other hand, elective etching of dentin reduces the bond strength of RelyX Unicem with the dentin. PMID:28979732

Comparison of the shear bond strength of self-adhesive resin cements to enamel and dentin with different protocol of application.

PubMed

Moghaddas, Mohammad Javad; Hossainipour, Zahra; Majidinia, Sara; Ojrati, Najmeh

2017-08-01

The aim of the present study was to determine the shear bond strength of self-adhesive resin cements to enamel and dentin with and without surface treatments, and compare them with conventional resin cement as the control group. In this experimental study, buccal and lingual surface of the thirty sound human premolars were polished in order to obtain a flat surface of enamel (E) in buccal, and dentin (D) in lingual. Sixty feldspathic ceramic blocks (2×3×3 mm) were prepared and randomly divided into six groups (n=10). Each block was cemented to the prepared surface (30 enamel and 30 dentin surface) according to different protocol: E1 and D1; RelyX ARC as control group, E2, D2; RelyX Unicem, E3, D3; acid etching +RelyX Unicem. The specimens were termocycled and subjected to shear forces by a universal testing machine at a cross head speed of 0.5 mm/min. The mode of fracture were evaluated by stereomicroscope. Data were analyzed with descriptive statistical methods using SPSS version 15. One-way ANOVA, and post hoc Tukey tests were used to compare bond strengths between the groups with different adhesives at α=0.05. Statistical analysis showed no significant differences within the enamel subgroups, but there were significant differences within the dentinal subgroups, and statistically significant differences were found between the groups D1and D3 (p=0.02). Comparison between similar enamel and dentinal subgroups showed that there was a significant difference just between the subgroups E3 and D3 (p=0.01). Elective etching of enamel did not lead to significant increase in the shear bond strength of RelyX Unicem in comparison to RelyX ARC. On the other hand, elective etching of dentin reduces the bond strength of RelyX Unicem with the dentin.

Investigating the generation of Love waves in secondary microseisms using 3D numerical simulations

NASA Astrophysics Data System (ADS)

Wenk, Stefan; Hadziioannou, Celine; Pelties, Christian; Igel, Heiner

2014-05-01

Longuet-Higgins (1950) proposed that secondary microseismic noise can be attributed to oceanic disturbances by surface gravity wave interference causing non-linear, second-order pressure perturbations at the ocean bottom. As a first approximation, this source mechanism can be considered as a force acting normal to the ocean bottom. In an isotropic, layered, elastic Earth model with plain interfaces, vertical forces generate P-SV motions in the vertical plane of source and receiver. In turn, only Rayleigh waves are excited at the free surface. However, several authors report on significant Love wave contributions in the secondary microseismic frequency band of real data measurements. The reason is still insufficiently analysed and several hypothesis are under debate: - The source mechanism has strongest influence on the excitation of shear motions, whereas the source direction dominates the effect of Love wave generation in case of point force sources. Darbyshire and Okeke (1969) proposed the topographic coupling effect of pressure loads acting on a sloping sea-floor to generate the shear tractions required for Love wave excitation. - Rayleigh waves can be converted into Love waves by scattering. Therefore, geometric scattering at topographic features or internal scattering by heterogeneous material distributions can cause Love wave generation. - Oceanic disturbances act on large regions of the ocean bottom, and extended sources have to be considered. In combination with topographic coupling and internal scattering, the extent of the source region and the timing of an extended source should effect Love wave excitation. We try to elaborate the contribution of different source mechanisms and scattering effects on Love to Rayleigh wave energy ratios by 3D numerical simulations. In particular, we estimate the amount of Love wave energy generated by point and extended sources acting on the free surface. Simulated point forces are modified in their incident angle, whereas extended sources are adapted in their spatial extent, magnitude and timing. Further, the effect of variations in the correlation length and perturbation magnitude of a random free surface topography as well as an internal random material distribution are studied.

Single-molecule force measurements of the polymerizing dimeric subunit of von Willebrand factor

NASA Astrophysics Data System (ADS)

Wijeratne, Sithara S.; Li, Jingqiang; Yeh, Hui-Chun; Nolasco, Leticia; Zhou, Zhou; Bergeron, Angela; Frey, Eric W.; Moake, Joel L.; Dong, Jing-fei; Kiang, Ching-Hwa

2016-01-01

Von Willebrand factor (VWF) multimers are large adhesive proteins that are essential to the initiation of hemostatic plugs at sites of vascular injury. The binding of VWF multimers to platelets, as well as VWF proteolysis, is regulated by shear stresses that alter VWF multimeric conformation. We used single molecule manipulation with atomic force microscopy (AFM) to investigate the effect of high fluid shear stress on soluble dimeric and multimeric forms of VWF. VWF dimers are the smallest unit that polymerizes to construct large VWF multimers. The resistance to mechanical unfolding with or without exposure to shear stress was used to evaluate VWF conformational forms. Our data indicate that, unlike recombinant VWF multimers (RVWF), recombinant dimeric VWF (RDVWF) unfolding force is not altered by high shear stress (100 dynes/cm2 for 3 min at 37°C ). We conclude that under the shear conditions used (100 dynes/cm2 for 3 min at 37°C ) , VWF dimers do not self-associate into a conformation analogous to that attained by sheared large VWF multimers.

Wrinkling of Stretched Films: Shear Stress

NASA Technical Reports Server (NTRS)

Zak, M. A.

1982-01-01

Report presents theoretical investigation on nonlinear shearing characteristics of wrinkling films under applied shear stress. Report helps explain force/deflection characteristic of in-planeboom and solar-array blanket structural combinations.

Significance of Shear Wall in Multi-Storey Structure With Seismic Analysis

NASA Astrophysics Data System (ADS)

Bongilwar, Rajat; Harne, V. R.; Chopade, Aditya

2018-03-01

In past decades, shear walls are one of the most appropriate and important structural component in multi-storied building. Therefore, it would be very interesting to study the structural response and their systems in multi-storied structure. Shear walls contribute the stiffness and strength during earthquakes which are often neglected during design of structure and construction. This study shows the effect of shear walls which significantly affect the vulnerability of structures. In order to test this hypothesis, G+8 storey building was considered with and without shear walls and analyzed for various parameters like base shear, storey drift ratio, lateral displacement, bending moment and shear force. Significance of shear wall has been studied with the help of two models. First model is without shear wall i.e. bare frame and other another model is with shear wall considering opening also in it. For modeling and analysis of both the models, FEM based software ETABS 2016 were used. The analysis of all models was done using Equivalent static method. The comparison of results has been done based on same parameters like base shear, storey drift ratio, lateral displacement, bending moment and shear force.

Glycosyltransferase-programmed stereosubstitution (GPS) to create HCELL: engineering a roadmap for cell migration.

PubMed

Sackstein, Robert

2009-07-01

During evolution of the vertebrate cardiovascular system, the vast endothelial surface area associated with branching vascular networks mandated the development of molecular processes to efficiently and specifically recruit circulating sentinel host defense cells and tissue repair cells at localized sites of inflammation/tissue injury. The forces engendered by high-velocity blood flow commensurately required the evolution of specialized cell surface molecules capable of mediating shear-resistant endothelial adhesive interactions, thus literally capturing relevant cells from the blood stream onto the target endothelial surface and permitting subsequent extravasation. The principal effectors of these shear-resistant binding interactions comprise a family of C-type lectins known as 'selectins' that bind discrete sialofucosylated glycans on their respective ligands. This review explains the 'intelligent design' of requisite reagents to convert native CD44 into the sialofucosylated glycoform known as hematopoietic cell E-/L-selectin ligand (HCELL), the most potent E-selectin counter-receptor expressed on human cells, and will describe how ex vivo glycan engineering of HCELL expression may open the 'avenues' for the efficient vascular delivery of cells for a variety of cell therapies.

Role of upper-level wind shear on the structure and maintenance of derecho-producing convective systems

NASA Astrophysics Data System (ADS)

Coniglio, Michael Charles

Common large-scale environments associated with the development of derecho-producing convective systems from a large number of events are identified using statistical clustering of the 500-mb geopotential heights as guidance. The majority of the events (72%) fall into three main patterns that include a well-defined upstream trough (40%), a ridge (20%), and a zonal, low-amplitude flow (12%), which is defined as an additional warm-season pattern that is not identified in past studies of derecho environments. Through an analysis of proximity soundings, discrepancies are found in both low-level and deep-tropospheric shear parameters between observations and the shear profiles considered favorable for strong, long-lived convective systems in idealized simulations. To explore the role of upper-level shear in derecho environments, a set of two-dimensional simulations of density currents within a dry, neutrally stable environment are used to examine the ability of a cold pool to lift environmental air within a vertically sheared flow. The results confirm that the addition of upper-level shear to a wind profile with weak to moderate low-level shear increases the vertical displacement of low-level parcels despite a decrease in the vertical velocity along the cold pool interface, as suggested by previous studies. Parcels that are elevated above the surface (1-2 km) overturn and are responsible for the deep lifting in the deep-shear environments. This deep overturning caused by the upper-level shear helps to maintain the tilt of the convective systems in more complex two-dimensional and three dimensional simulations. The overturning also is shown to greatly increase the size of the convective systems in the three-dimensional simulations by facilitating the initiation and maintenance of convective cells along the cold pool. When combined with estimates of the cold pool motion and the storm-relative hodograph, these results may best be used for the prediction of the demise of strong, linear mesoscale convective systems (MCSs) and may provide a conceptual model for the persistence of strong MCSs above a surface nocturnal inversion in situations that are not forced by a low-level jet.

Role of 3D force networks in linking grain scale to macroscale processes in sheared granular debris

NASA Astrophysics Data System (ADS)

Mair, K.; Jettestuen, E.; Abe, S.

2013-12-01

Active faults, landslides and subglacial tills contain accumulations of granular debris that evolve during sliding. The macroscopic motion in these environments is at least to some extent determined by processes operating in this sheared granular material. A valid question is how the local behavior at the individual granular contacts actually sums up to influence macroscopic sliding. Laboratory experiments and numerical modeling can potentially help elucidate this. Observations of jamming (stick) and unjamming (flow) as well as concentrated shear bands on the scale of 5-10 grains suggest that a simple continuum description may be insufficient to capture important elements of the behavior. We therefore seek a measure of the organization of the granular fabric and the 3D structure of the load bearing skeleton that effectively demonstrates how the individual grain interactions are manifested in the macroscopic sliding behavior we observe. Contact force networks are an expression of this. Here we investigate the structure and variability of the most connected system spanning force networks produced in 3D discrete element models of granular layers under shear. We use percolation measures to identify, characterize, compare and track the evolution of these strongly connected contact force networks. We show that specific topological measures used in describing the networks, such as number of contacts and coordination number, are sensitive to grain size distribution (and likely the grain shape) of the material as well as loading conditions. Hence, faults of different maturity would be expected to accommodate shear in different ways. Distinct changes in the topological characteristics i.e. the geometry of strong force networks with accumulated strain are directly correlated to fluctuations in macroscopic shearing resistance. This suggests that 3D force networks play an important bridging role between individual grain scale processes and macroscopic sliding behavior.

Mechanisms of anterior-posterior stability of the knee joint under load-bearing.

PubMed

Reynolds, Ryan J; Walker, Peter S; Buza, John

2017-05-24

The anterior-posterior (AP) stability of the knee is an important aspect of functional performance. Studies have shown that the stability increases when compressive loads are applied, as indicated by reduced laxity, but the mechanism has not been fully explained. A test rig was designed which applied combinations of AP shear and compressive forces, and measured the AP displacements relative to the neutral position. Five knees were evaluated at compressive loads of 0, 250, 500, and 750N, with the knee at 15° flexion. At each load, three cycles of shear force at ±100N were applied. For the intact knee under load, the posterior tibial displacement was close to zero, due to the upward slope of the anterior medial tibial surface. The soft tissues were then resected in sequence to determine their role in AP laxity. After anterior cruciate ligament (ACL) resection, the anterior tibial displacement increased significantly even under load, highlighting its importance in stability. Meniscal resection further increased displacement but also the vertical displacement increased, implying the meniscus was providing a buffering effect. The PCL had no effect on any of the displacements under load. Plowing cartilage deformation and surface friction were negligible. This work highlighted the particular importance of the upward slope of the anterior medial tibial surface and the ACL to AP knee stability under load. The results are relevant to the design of total knees which reproduce anatomic knee stability behavior. Copyright © 2017. Published by Elsevier Ltd.

Transverse shear effect in a circumferentially cracked cylindrical shell

NASA Technical Reports Server (NTRS)

Delale, F.; Erdogan, F.

1979-01-01

The objectives of the paper are to solve the problem of a circumferentially-cracked cylindrical shell by taking into account the effect of transverse shear, and to obtain the stress intensity factors for the bending moment as well as the membrane force as the external load. The formulation of the problem is given for a specially orthotropic material within the framework of a linearized shallow shell theory. The particular theory used permits the consideration of all five boundary conditions as to moment and stress resultants on the crack surface. The effect of Poisson's ratio on the stress intensity factors and the nature of the out-of-plane displacement along the edges of the crack, i.e., bulging, are also studied.

Fast low-to-high confinement mode bifurcation dynamics in a tokamak edge plasma gyrokinetic simulation

DOE PAGES

Chang, C. S.; Ku, S.; Tynan, G. R.; ...

2017-04-25

Transport barrier formation and its relation to sheared flows in fluids and plasmas are of fundamental interest in various natural and laboratory observations and of critical importance in achieving an economical energy production in a magnetic fusion device. Here we report the first observation of an edge transport barrier formation event in an electrostatic gyrokinetic simulation carried out in a realistic diverted tokamak edge geometry under strong forcing by a high rate of heat deposition. Here, the results show that turbulent Reynolds-stress-driven sheared E x B flows act in concert with neoclassical orbit loss to quench turbulent transport and formmore » a transport barrier just inside the last closed magnetic flux surface.« less

Stacking Faults and Mechanical Behavior beyond the Elastic Limit of an Imidazole-Based Metal Organic Framework: ZIF-8.

PubMed

Hegde, Vinay I; Tan, Jin-Chong; Waghmare, Umesh V; Cheetham, Anthony K

2013-10-17

We determine the nonlinear mechanical behavior of a prototypical zeolitic imidazolate framework (ZIF-8) along two modes of mechanical failure in response to tensile and shear forces using first-principles simulations. Our generalized stacking fault energy surface reveals an intrinsic stacking fault of surprisingly low energy comparable to that in copper, though the energy barrier associated with its formation is much higher. The lack of vibrational spectroscopic evidence for such faults in experiments can be explained with the structural instability of the barrier state to form a denser and disordered state of ZIF-8 seen in our analysis, that is, large shear leads to its amorphization rather than formation of faults.

Modeling of traction-coupling properties of wheel propulsor

NASA Astrophysics Data System (ADS)

Sakhapov, R. L.; Nikolaeva, R. V.; Gatiyatullin, M. H.; Makhmutov, M. M.

2017-12-01

In conditions of operation of aggregates on soils with low bearing capacity, the main performance indicators of their operation are determined by the properties of retaining the functional qualities of the propulsor. Therefore, the parameters of the anti-skid device can not be calculated by only one criterion. The equipment of propellers with anti-skid devices, which allow to reduce the compaction effect of the propulsion device on the soil, seems to be a rational solution to the problem of increasing traction and coupling properties of the driving wheels. The mathematical model is based on the study of the interaction of the driving wheel with anti-skid devices and a deformable bearing surface, which takes into account the wheel diameter, skid coefficient, the parameters of the anti-skid device, the physical and mechanical properties of the soil. As a basic mathematical model that determines the dependence of the coupling properties on the wheel parameters, the model obtained as a result of integration and reflecting the process of soil deformation from the shear stress is adopted. The total value of the resistance forces will determine the force of the hitch pressure on the horizontal soil layers, and the value of its deformation is the degree of wheel slippage. When the anti-skid devices interact with the soil, the traction capacity of the wheel is composed of shear forces, soil shear and soil deformation forces with detachable hooks. As a result of the interaction of the hook with the soil, the latter presses against the walls of the hook with the force equal to the sum of the hook load and the resistance to movement. During operation, the linear dimensions of the hook will decrease, which is not taken into account by the safety factor. Abrasive wear of the thickness of the hook is approximately proportional to the work of friction caused by the movement of the hook when inserted into the soil and slipping the wheel.

Cavity-actuated supersonic mixing and combustion control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yu, K.H.; Schadow, K.C.

1994-11-01

Compressible shear layers in supersonic jets are quite stable and spread very slowly compared with incompressible shear layers. In this paper, a novel use of a cavity-actuated forcing technique is demonstrated for increasing the spreading rate of compressible shear layers. Periodic modulations were applied to Mach 2.0 reacting and nonreacting jets using the cavities that were attached at the exit of a circular supersonic nozzle. The effect of cavity-actuated forcing was studied as a function of the cavity geometry, in particular, the length and the depth of the cavity. When the cavities were tuned to certain frequencies, large-scale highly coherentmore » structures were produced in the shear layers substantially increasing the growth rate. The cavity excitation was successfully applied to both cold and hot supersonic jets. When applied to cold Mach 2.0 air jets. the cavity-actuated forcing increased the spreading rate of the initial shear layers with the convective Mach number (M[sub C]) of 0.85 by a factor of three. For high-temperature Mach 2.0 jets with M[sub C] of 1.4, a 50% increase in the spreading rate was observed with the forcing. Finally, the cavity-actuated forcing was applied to reacting supersonic jets with ethylene-oxygen afterburning. For this case, the forcing caused a 20%--30% reduction in the afterburning flame length and modified the afterburning intensity significantly. The direction of the modification depended on the characteristics of the afterburning flames. The intensity was reduced with forcing for unstable flames with weak afterburning while it was increased for stable flames with strong afterburning.« less

Preliminary Rotary Wing Full Spectrum Crashworthiness Criteria

DTIC Science & Technology

2010-01-01

6.2.1 Injury Risk Due to Occupant Loads Cervical forces and moments are to be used to evaluate injury to the head/neck, torso acceleration is used to...injury) for dynamic neck tension (lifting forces) at the occipital condyles (C0-C1, upper neck) and cervical vertebrae (C7-T1, lower neck) are defined...Neck Compression and Shear Force Limits The maximum acceptable cervical compression and shear force limits are defined in the following table

Investigation of the effects of shear on arc-electrode erosion using a modified arc-electrode mass loss model

NASA Astrophysics Data System (ADS)

Webb, Bryan T.

The electrodes are the attachment points for an electric arc where electrons and positive ions enter and leave the gas, creating a flow of current. Electrons enter the gas at the cathode and are removed at the anode. Electrons then flow out through the leads on the anode and are replenished from the power supply through the leads on the cathode. Electric arc attachment to the electrode surface causes intensive heating and subsequent melting and vaporization. At that point a multitude of factors can contribute to mass loss, to include vaporization (boiling), material removal via shear forces, chemical reactions, evaporation, and ejection of material in jets due to pressure effects. If these factors were more thoroughly understood and could be modeled, this knowledge would guide the development of an electrode design with minimal erosion. An analytic model was developed by a previous researcher that models mass loss by melting, evaporation and boiling with a moving arc attachment point. This pseudo one-dimensional model includes surface heat flux in periodic cycles of heating and cooling to model motion of a spinning arc in an annular electrode where the arc periodically returns to the same spot. This model, however, does not account for removal of material due to shear or pressure induced effects, or the effects of chemical reactions. As a result of this, the model under-predicts material removal by about 50%. High velocity air flowing over an electrode will result in a shear force which has the potential to remove molten material as the arc melts the surface on its path around the electrode. In order to study the effects of shear on mass loss rate, the model from this previous investigator has been altered to include this mass loss mechanism. The results of this study have shown that shear is a viable mechanism for mass loss in electrodes and can account for the mismatch between theoretical and experimental rates determined by previous investigators. The results of a parametric study of arc attachment factors - including spot size, fall voltage, arc spot rotation rate, ambient bore heat rate, and air mass flow rate - are presented. The parametric study resulted in improving estimates of both the arc spot size and electrode fall voltage, two critical factors affecting electrode heating. Little sensitivity of electrode erosion rate to ambient bore heat rate and rotation rate was found. The erosion rate is found to be sensitive to the mass flow rate of air injected in the arc heater and validation of the model by comparison with more run condition data should be carried out as the data become available.

The effect of physiological conditions on the surface structure of proteins: Setting the scene for human digestion of emulsions

NASA Astrophysics Data System (ADS)

Maldonado-Valderrama, J.; Gunning, A. P.; Ridout, M. J.; Wilde, P. J.; Morris, V. J.

2009-10-01

Understanding and manipulating the interfacial mechanisms that control human digestion of food emulsions is a crucial step towards improved control of dietary intake. This article reports initial studies on the effects of the physiological conditions within the stomach on the properties of the film formed by the milk protein ( β -lactoglobulin) at the air-water interface. Atomic force microscopy (AFM), surface tension and surface rheology techniques were used to visualize and examine the effect of gastric conditions on the network structure. The effects of changes in temperature, pH and ionic strength on a pre-formed interfacial structure were characterized in order to simulate the actual digestion process. Changes in ionic strength had little effect on the surface properties. In isolation, acidification reduced both the dilatational and the surface shear modulus, mainly due to strong repulsive electrostatic interactions within the surface layer and raising the temperature to body temperature accelerated the rearrangements within the surface layer, resulting in a decrease of the dilatational response and an increase of surface pressure. Together pH and temperature display an unexpected synergism, independent of the ionic strength. Thus, exposure of a pre-formed interfacial β -lactoglobulin film to simulated gastric conditions reduced the surface dilatational modulus and surface shear moduli. This is attributed to a weakening of the surface network in which the surface rearrangements of the protein prior to exposure to gastric conditions might play a crucial role.

Effect of vertical ground motions on shear demand and capacity in bridge columns.

DOT National Transportation Integrated Search

2012-03-01

The objective of this project was to examine the effects of axial force variation in bridge columns due to strong vertical : ground motions and the influence of these axial force fluctuations on shear strength degradation. : Two quarter scale specime...

Increase in Mechanical Resistance to Force in a Shear-Activated Protein

NASA Astrophysics Data System (ADS)

Botello, Eric; Harris, Nolan; Choi, Huiwan; Zhou, Zhou; Bergeron, Angela; Dong, Jing-Fei; Kiang, Ching-Hwa

2009-03-01

von Willebrand factor (VWF) is the largest multimeric adhesion ligand found in human blood. Plasma VWF (pVWF) must be exposed to shear stress, like at sites of vascular injury, to be activated to bind platelets to induce blood clotting. In addition, adhesion activity of VWF is related to its polymer size, with the ultra-large form of VWF (ULVWF) being hyper-active, and forming fibers even without exposure to shear stress. We used the AFM to stretch pVWF, sheared VWF (sVWF) and ULVWF, and monitor the forces as a function of molecular extension. We showed a similar increase in force resistance to unfolding for sVWF and ULVWF when compared to pVWF. The increase in force is reduced when other molecules that are known to disrupt their fibril formation are present. Our results provide evidence that the common higher order structure of sVWF and ULVWF may affect the domain structure that causes difference in their adhesion activity compared to pVWF.

Meat quality attributes of Agile Wallabies.

PubMed

Geesink, Geert H; van den Heuvel, Aaron; Hunt, Warren

2017-11-01

Meat quality traits of Agile Wallaby (Macropus agilis) M. longissimus (loin) and M. semimembranosus (topside) were investigated. Both muscles exhibited a relatively high pH (>5.7) and dark colour (L*-, a*-, and b*-values). Aging the loins from 2 to 21days p.m. had a significant effect on shear force. However, the results regarding shear force, myofibrillar fragmentation index (MFI) and degradation of desmin and troponin-T suggested that the aging response largely occurred within 2days p.m. Suspension of carcasses from one leg resulted in a side effect on shear force of the loin at 2 and 7days p.m., but not on sarcomere length or MFI. Topsides from the free hanging leg exhibited lower shear force values (33 vs 42N) and greater sarcomere lengths (2.51 vs 1.84μM). Tenderness, juiciness, flavour and overall liking were higher for loins than topsides. Sensory scores for the loin and topside were slightly lower and similar, respectively, to those reported for lamb. Copyright © 2017 Elsevier Ltd. All rights reserved.

Statistics of acoustic emissions and stress drops during granular shearing using a stick-slip fiber bundle mode

NASA Astrophysics Data System (ADS)

Cohen, D.; Michlmayr, G.; Or, D.

2012-04-01

Shearing of dense granular materials appears in many engineering and Earth sciences applications. Under a constant strain rate, the shearing stress at steady state oscillates with slow rises followed by rapid drops that are linked to the build up and failure of force chains. Experiments indicate that these drops display exponential statistics. Measurements of acoustic emissions during shearing indicates that the energy liberated by failure of these force chains has power-law statistics. Representing force chains as fibers, we use a stick-slip fiber bundle model to obtain analytical solutions of the statistical distribution of stress drops and failure energy. In the model, fibers stretch, fail, and regain strength during deformation. Fibers have Weibull-distributed threshold strengths with either quenched and annealed disorder. The shape of the distribution for drops and energy obtained from the model are similar to those measured during shearing experiments. This simple model may be useful to identify failure events linked to force chain failures. Future generalizations of the model that include different types of fiber failure may also allow identification of different types of granular failures that have distinct statistical acoustic emission signatures.

High-speed AFM and the reduction of tip-sample forces

NASA Astrophysics Data System (ADS)

Miles, Mervyn; Sharma, Ravi; Picco, Loren

High-speed DC-mode AFM has been shown to be routinely capable of imaging at video rate, and, if required, at over 1000 frames per second. At sufficiently high tip-sample velocities in ambient conditions, the tip lifts off the sample surface in a superlubricity process which reduces the level of shear forces imposed on the sample by the tip and therefore reduces the potential damage and distortion of the sample being imaged. High-frequency mechanical oscillations, both lateral and vertical, have been reported to reduced the tip-sample frictional forces. We have investigated the effect of combining linear high-speed scanning with these small amplitude high-frequency oscillations with the aim of reducing further the force interaction in high-speed imaging. Examples of this new version of high-speed AFM imaging will be presented for biological samples.

Shear properties evaluation of a truss core of sandwich beams

NASA Astrophysics Data System (ADS)

Wesolowski, M.; Ludewicz, J.; Domski, J.; Zakrzewski, M.

2017-10-01

The open-cell cores of sandwich structures are locally bonded to the face layers by means of adhesive resin. The sandwich structures composed of different parent materials such as carbon fibre composites (laminated face layers) and metallic core (aluminium truss core) brings the need to closely analyse their adhesive connections which strength is dominated by the shear stress. The presented work considers sandwich beams subjected to the static tests in the 3-point bending with the purpose of estimation of shear properties of the truss core. The main concern is dedicated to the out-of plane shear modulus and ultimate shear stress of the aluminium truss core. The loading of the beam is provided by a static machine. For the all beams the force - deflection history is extracted by means of non-contact optical deflection measurement using PONTOS system. The mode of failure is identified for each beam with the corresponding applied force. A flexural rigidity of the sandwich beams is also discussed based on force - displacement plots.

Mechanisms for Flow-Enhanced Cell Adhesion

PubMed Central

Zhu, Cheng; Yago, Tadayuki; Lou, Jizhong; Zarnitsyna, Veronika I.; McEver, Rodger P.

2009-01-01

Cell adhesion is mediated by specific receptor—ligand bonds. In several biological systems, increasing flow has been observed to enhance cell adhesion despite the increasing dislodging fluid shear forces. Flow-enhanced cell adhesion includes several aspects: flow augments the initial tethering of flowing cells to a stationary surface, slows the velocity and increases the regularity of rolling cells, and increases the number of rollingly adherent cells. Mechanisms for this intriguing phenomenon may include transport-dependent acceleration of bond formation and force-dependent deceleration of bond dissociation. The former includes three distinct transport modes: sliding of cell bottom on the surface, Brownian motion of the cell, and rotational diffusion of the interacting molecules. The latter involves a recently demonstrated counterintuitive behavior called catch bonds where force prolongs rather than shortens the lifetimes of receptor—ligand bonds. In this article, we summarize our recently published data that used dimensional analysis and mutational analysis to elucidate the above mechanisms for flow-enhanced leukocyte adhesion mediated by L-selectinligand interactions. PMID:18299992

Wedge disclination dipole in an embedded nanowire within the surface/interface elasticity

NASA Astrophysics Data System (ADS)

Shodja, Hossein M.; Rezazadeh-Kalehbasti, Shaghayegh; Gutkin, Mikhail Yu

2013-12-01

The elastic behavior of an arbitrary oriented wedge disclination dipole located inside a nanowire, which in turn is embedded in an infinite matrix, is studied within the surface/interface theory of elasticity. The corresponding boundary value problem is provided using complex potential functions. The potential functions are defined through modeling the wedge disclination in terms of an equivalent distribution of edge dislocations. The interface effects on the stress field and strain energy of the disclination dipole and image forces acting on it, the influence of relative shear moduli of the nanowire and the matrix, as well as the different characteristics of the interface are studied thoroughly. It is shown that the positive interface modulus leads to increased strain energy and extra repulsive forces on the disclination dipole. The noticeable effect of the negative interface modulus is the non-classical oscillations in the stress field of the disclination dipole and an extra attractive image force on it.

Mechanosensing drives acuity of αβ T-cell recognition

PubMed Central

Feng, Yinnian; Brazin, Kristine N.; Kobayashi, Eiji; Mallis, Robert J.; Reinherz, Ellis L.; Lang, Matthew J.

2017-01-01

T lymphocytes use surface αβ T-cell receptors (TCRs) to recognize peptides bound to MHC molecules (pMHCs) on antigen-presenting cells (APCs). How the exquisite specificity of high-avidity T cells is achieved is unknown but essential, given the paucity of foreign pMHC ligands relative to the ubiquitous self-pMHC array on an APC. Using optical traps, we determine physicochemical triggering thresholds based on load and force direction. Strikingly, chemical thresholds in the absence of external load require orders of magnitude higher pMHC numbers than observed physiologically. In contrast, force applied in the shear direction (∼10 pN per TCR molecule) triggers T-cell Ca2+ flux with as few as two pMHC molecules at the interacting surface interface with rapid positional relaxation associated with similarly directed motor-dependent transport via ∼8-nm steps, behaviors inconsistent with serial engagement during initial TCR triggering. These synergistic directional forces generated during cell motility are essential for adaptive T-cell immunity against infectious pathogens and cancers. PMID:28811364

Force-Induced Rupture of a DNA Duplex: From Fundamentals to Force Sensors.

PubMed

Mosayebi, Majid; Louis, Ard A; Doye, Jonathan P K; Ouldridge, Thomas E

2015-12-22

The rupture of double-stranded DNA under stress is a key process in biophysics and nanotechnology. In this article, we consider the shear-induced rupture of short DNA duplexes, a system that has been given new importance by recently designed force sensors and nanotechnological devices. We argue that rupture must be understood as an activated process, where the duplex state is metastable and the strands will separate in a finite time that depends on the duplex length and the force applied. Thus, the critical shearing force required to rupture a duplex depends strongly on the time scale of observation. We use simple models of DNA to show that this approach naturally captures the observed dependence of the force required to rupture a duplex within a given time on duplex length. In particular, this critical force is zero for the shortest duplexes, before rising sharply and then plateauing in the long length limit. The prevailing approach, based on identifying when the presence of each additional base pair within the duplex is thermodynamically unfavorable rather than allowing for metastability, does not predict a time-scale-dependent critical force and does not naturally incorporate a critical force of zero for the shortest duplexes. We demonstrate that our findings have important consequences for the behavior of a new force-sensing nanodevice, which operates in a mixed mode that interpolates between shearing and unzipping. At a fixed time scale and duplex length, the critical force exhibits a sigmoidal dependence on the fraction of the duplex that is subject to shearing.

Epidermolysis bullosa: Careful monitoring and no touch principle for anesthesia management.

PubMed

Saraf, Sujit V; Mandawade, Nishigandha J; Gore, Sandeep K; Padhye, Usha D; Pereira, Charissa S

2013-07-01

Epidermolysis bullosa (EB) is a rare genetic mechanobullous disorder, with excessive fragility of the skin and mucous membranes. Avoiding mechanical injury to the skin and mucous membranes is essential in the anesthetic management. Shearing forces applied to the skin result in bullae formation, while compressive forces to the skin are tolerated. The challenge is to use monitoring technology without damaging the epithelial surface. Difficult airway, positioning issues, nutritional deficiencies, poor immunity, and carcinogenic potential add to the comorbidities. We managed a child with EB undergoing syndactyly release. Ensuring maximal skin and mucous membrane protection, anesthesia in children with EB can be conducted with few sequelae.

Nanospectrofluorometry inside single living cell by scanning near-field optical microscopy

NASA Astrophysics Data System (ADS)

Lei, F. H.; Shang, G. Y.; Troyon, M.; Spajer, M.; Morjani, H.; Angiboust, J. F.; Manfait, M.

2001-10-01

Near-field fluorescence spectra with subdiffraction limit spatial resolution have been taken in the proximity of mitochondrial membrane inside breast adenocarcinoma cells (MCF7) treated with the fluorescent dye (JC-1) by using a scanning near-field optical microscope coupled with a confocal laser microspectrofluorometer. The probe-sample distance control is based on a piezoelectric bimorph shear force sensor having a static spring constant k=5 μN/nm and a quality factor Q=40 in a physiological medium of viscosity η=1.0 cp. The sensitivity of the force sensor has been tested by imaging a MCF7 cell surface.

Driving reconnection in sheared magnetic configurations with forced fluctuations

NASA Astrophysics Data System (ADS)

Pongkitiwanichakul, Peera; Makwana, Kirit D.; Ruffolo, David

2018-02-01

We investigate reconnection of magnetic field lines in sheared magnetic field configurations due to fluctuations driven by random forcing by means of numerical simulations. The simulations are performed with an incompressible, pseudo-spectral magnetohydrodynamics code in 2D where we take thick, resistively decaying, current-sheet like sheared magnetic configurations which do not reconnect spontaneously. We describe and test the forcing that is introduced in the momentum equation to drive fluctuations. It is found that the forcing does not change the rate of decay; however, it adds and removes energy faster in the presence of the magnetic shear structure compared to when it has decayed away. We observe that such a forcing can induce magnetic reconnection due to field line wandering leading to the formation of magnetic islands and O-points. These reconnecting field lines spread out as the current sheet decays with time. A semi-empirical formula is derived which reasonably explains the formation and spread of O-points. We find that reconnection spreads faster with stronger forcing and longer correlation time of forcing, while the wavenumber of forcing does not have a significant effect. When the field line wandering becomes large enough, the neighboring current sheets with opposite polarity start interacting, and then the magnetic field is rapidly annihilated. This work is useful to understand how forced fluctuations can drive reconnection in large scale current structures in space and astrophysical plasmas that are not susceptible to reconnection.

Aerodynamics of a translating comb-like plate inspired by a fairyfly wing

NASA Astrophysics Data System (ADS)

Lee, Seung Hun; Kim, Daegyoum

2017-08-01

Unlike the smooth wings of common insects or birds, micro-scale insects such as the fairyfly have a distinctive wing geometry, comprising a frame with several bristles. Motivated by this peculiar wing geometry, we experimentally investigated the flow structure of a translating comb-like wing for a wide range of gap size, angle of attack, and Reynolds number, Re = O(10) - O(103), and the correlation of these parameters with aerodynamic performance. The flow structures of a smooth plate without a gap and a comb-like plate are significantly different at high Reynolds number, while little difference was observed at the low Reynolds number of O(10). At low Reynolds number, shear layers that were generated at the edges of the tooth of the comb-like plate strongly diffuse and eventually block a gap. This gap blockage increases the effective surface area of the plate and alters the formation of leading-edge and trailing-edge vortices. As a result, the comb-like plate generates larger aerodynamic force per unit area than the smooth plate. In addition to a quasi-steady phase after the comb-like plate travels several chords, we also studied a starting phase of the shear layer development when the comb-like plate begins to translate from rest. While a plate with small gap size can generate aerodynamic force at the starting phase as effectively as at the quasi-steady phase, the aerodynamic force drops noticeably for a plate with a large gap because the diffusion of the developing shear layers is not enough to block the gap.

Temperature-dependent residual shear strength characteristics of smectite-rich landslide soils

NASA Astrophysics Data System (ADS)

Shibasaki, Tatsuya; Matsuura, Sumio; Okamoto, Takashi

2015-04-01

On gentle clayey slopes in weathered argillaceous rock areas, there exist many landslides which repeatedly reactivate with slow movement. The slip surface soils of these landslides are sometimes composed dominantly of swelling clay mineral (smectite) which is well known to show extremely low residual friction angle. From field data monitored at landslide sites in Japan, it has become clear that some landslides with relatively shallow slip surface begin to move and become active in late autumn or early winter every year. In such cases, the triggering mechanisms of landslides have not been understood well enough, because landslide initiation and movement are not always clearly linked with rises in pore water pressures (ground water levels). In this study, we focus on the influence of seasonal variation in ground temperature on slope stability and have investigated the effect of temperature on the shear strength of slip surface soils. Undisturbed soil samples were collected by boring from the Busuno landslide in Japan. We performed box shear experiments on undisturbed slip surface soils at low temperature ranges (approximately 5-25 °C). XRD analysis revealed that these soils contain high fraction of smectite. Slickensided slip surface within test specimen was coincided with the shearing plane of the shear box and shear displacement was applied precisely along the localized slip surface. Experiments were performed under slow shearing rate condition (0.005mm/min) and the results showed that shear strength decreased with decreasing temperature. Temperature effect was rather significant on frictional angle than on cohesion. Ring shear experiments were also performed on normally-consolidated remoulded samples. Under residual strength condition, temperature-change experiments (cooling-event tests) ranging approximately from 5 to 25 °C were performed on smectite-rich landslide soils and commercial bentonites. As well as the results by box shear test, shear weakening behaviors were also recognized during cooling-event tests. Shear stress fluctuations, which were obtained by 1 Hz data sampling, showed that shear behavior characteristically changed in response to temperature conditions. Stick-slip behavior prevailed under room temperature conditions, whereas shear behavior gradually changed into stable sliding behavior as temperature decreased. SEM (Scanning Electric Microscope) observation on shear surfaces indicated that silt- and sand-size asperities in the vicinity of the shear surface influence the occurrence of stick-slip behavior. It is also characteristically noted that rod-shaped smectitic clays, here called "roll", developed on shear surfaces and are arrayed densely perpendicular to the shearing direction in a micrometer scale. We assume that these rolls are probably rotating slowly within shear zone and acting as a lubricant which affects the temperature-dependent frictional properties of the shearing plane. These experimental results show that residual strength characteristics of smectite-rich soils are sensitive to temperature conditions. Our findings imply that if slip surface soils contain a high fraction of smectite, a decrease in ground temperature can lead to lowered shear resistance of the slip surface and triggering of slow landslide movement.

Adhesion, friction and micromechanical properties of ceramics

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa

1988-01-01

The adhesion, friction, and micromechanical properties of ceramics, both in monolithic and coating form, are reviewed. Ceramics are examined in contact with themselves, other harder materials, and metals. For the simplicity of discussion, the tribological properties of concern in the processes are separated into two parts. The first part discusses the pull-off force (adhesion) and the shear force required to break the interfacial junctions between contacting surfaces. The role of chemical bonding in adhesion and friction, and the effects of surface contaminant films and temperature on tribological response with respect to adhesion and friction are discussed. The second part deals with abrasion of ceramics. Elastic, plastic, and fracture behavior of ceramics in solid state contact is discussed. The scratch technique of determining the critical load needed to fracture interfacial adhesive bonds of ceramic deposited on substrates is also addressed.

Fundamental tribological properties of ion-beam-deposited boron nitride films

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa

1989-01-01

The adhesion, friction, and micromechanical properties of ion-beam-deposited boron nitride (BN) films are reviewed. The BN films are examined in contact with BN metals and other harder materials. For simplicity of discussion, the tribological properties of concern in the processes are separated into two parts. First, the pull-off force (adhesion) and the shear force required to break the interfacial junctions between contacting surfaces are discussed. The effects of surface films, hardness of metals, and temperature on tribological response with respect to adhesion and friction are considered. The second part deals with the abrasion of the BN films. Elastic, plastic, and fracture behavior of the BN films in solid-state contact are discussed. The scratch technique of determining the critical load needed to fracture interfacial adhesive bonds of BN films deposited on substrates is also addressed.

Fundamental tribological properties of ion-beam-deposited boron nitride films

NASA Technical Reports Server (NTRS)

Miyoshi, K.

1990-01-01

The adhesion, friction, and micromechanical properties of ion-beam-deposited boron nitride (BN) films are reviewed. The BN films are examined in contact with BN metals and other harder materials. For simplicity of discussion, the tribological properties of concern in the processes are separated into two parts. First, the pull-off force (adhesion) and the shear force required to break the interfacial junctions between contacting surfaces are discussed. The effects of surface films, hardness of metals, and temperature on tribological response with respect to adhesion and friction are considered. The second part deals with the abrasion of the BN films. Elastic, plastic, and fracture behavior of the BN films in solid-state contact are discussed. The scratch technique of determining the critical load needed to fracture interfacial adhesive bonds of BN films deposited on substrates is also addressed.

Corona anemometry for qualitative measurement of reversing surface flow with application to separation control by external excitation

NASA Technical Reports Server (NTRS)

Durbin, P. A.; Mckinzie, D. J.

1987-01-01

An corona anemometer which detects gas flow by the displacement of an ion beam is described, and experiments are performed using the anemometer to investigate the active control of diffusor separation by periodic forcing. The apparatus is applied to the separated flow over a rearward facing ramp. An oscillating vane is attached to the surface near the separation point. It is suggested that the enhancement in turbulent energy produced by the oscillating vane is due to drastic modification of the wake shear flow, and not to vane-produced turbulence.

Shear-Sensitive Liquid Crystal Coating Method Applied Through Transparent Test Surfaces

NASA Technical Reports Server (NTRS)

Reda, Daniel C.; Wilder, Michael C.

1999-01-01

Research conducted at NASA Ames Research Center has shown that the color-change response of a shear-sensitive liquid crystal coating (SSLCC) to aerodynamic shear depends on both the magnitude of the local shear vector and its direction relative to the observer's in-plane line of sight. In conventional applications, the surface of the SSLCC exposed to aerodynamic shear is illuminated with white light from the normal direction and observed from an oblique above-plane view angle of order 30 deg. In this top-light/top-view mode, shear vectors with components directed away from the observer cause the SSLCC to exhibit color-change responses. At any surface point, the maximum color change (measured from the no-shear red or orange color) always occurs when the local vector is aligned with, and directed away from, the observer. The magnitude of the color change at this vector-observer-aligned orientation scales directly with shear stress magnitude. Conversely, any surface point exposed to a shear vector with a component directed toward the observer exhibits a non-color-change response, always characterized by a rusty-red or brown color, independent of both shear magnitude and direction. These unique, highly directional color-change responses of SSLCCs to aerodynamic shear allow for the full-surface visualization and measurement of continuous shear stress vector distributions. The objective of the present research was to investigate application of the SSLCC method through a transparent test surface. In this new back-light/back-view mode, the exposed surface of the SSLCC would be subjected to aerodynamic shear stress while the contact surface between the SSLCC and the solid, transparent wall would be illuminated and viewed in the same geometrical arrangement as applied in conventional applications. It was unknown at the outset whether or not color-change responses would be observable from the contact surface of the SSLCC, and, if seen, how these color-change responses might relate to those observed in standard practice.

Loss tangent and complex modulus estimated by acoustic radiation force creep and shear wave dispersion

PubMed Central

Amador, Carolina; Urban, Matthew W; Chen, Shigao; Greenleaf, James F

2012-01-01

Elasticity imaging methods have been used to study tissue mechanical properties and have demonstrated that tissue elasticity changes with disease state. In current shear wave elasticity imaging methods typically only shear wave speed is measured and rheological models, e.g., Kelvin-Voigt, Maxwell and Standard Linear Solid, are used to solve for tissue mechanical properties such as the shear viscoelastic complex modulus. This paper presents a method to quantify viscoelastic material properties in a model-independent way by estimating the complex shear elastic modulus over a wide frequency range using time-dependent creep response induced by acoustic radiation force. This radiation force induced creep (RFIC) method uses a conversion formula that is the analytic solution of a constitutive equation. The proposed method in combination with Shearwave Dispersion Ultrasound Vibrometry (SDUV) is used to measure the complex modulus so that knowledge of the applied radiation force magnitude is not necessary. The conversion formula is shown to be sensitive to sampling frequency and the first reliable measure in time according to numerical simulations using the Kelvin-Voigt model creep strain and compliance. Representative model-free shear complex moduli from homogeneous tissue mimicking phantoms and one excised swine kidney were obtained. This work proposes a novel model-free ultrasound-based elasticity method that does not require a rheological model with associated fitting requirements. PMID:22345425

Loss tangent and complex modulus estimated by acoustic radiation force creep and shear wave dispersion.

PubMed

Amador, Carolina; Urban, Matthew W; Chen, Shigao; Greenleaf, James F

2012-03-07

Elasticity imaging methods have been used to study tissue mechanical properties and have demonstrated that tissue elasticity changes with disease state. In current shear wave elasticity imaging methods typically only shear wave speed is measured and rheological models, e.g. Kelvin-Voigt, Maxwell and Standard Linear Solid, are used to solve for tissue mechanical properties such as the shear viscoelastic complex modulus. This paper presents a method to quantify viscoelastic material properties in a model-independent way by estimating the complex shear elastic modulus over a wide frequency range using time-dependent creep response induced by acoustic radiation force. This radiation force induced creep method uses a conversion formula that is the analytic solution of a constitutive equation. The proposed method in combination with shearwave dispersion ultrasound vibrometry is used to measure the complex modulus so that knowledge of the applied radiation force magnitude is not necessary. The conversion formula is shown to be sensitive to sampling frequency and the first reliable measure in time according to numerical simulations using the Kelvin-Voigt model creep strain and compliance. Representative model-free shear complex moduli from homogeneous tissue mimicking phantoms and one excised swine kidney were obtained. This work proposes a novel model-free ultrasound-based elasticity method that does not require a rheological model with associated fitting requirements.

Rapid distortion analysis of high speed homogeneous turbulence subject to periodic shear

DOE PAGES

Bertsch, Rebecca L.; Girimaji, Sharath S.

2015-12-30

The effect of unsteady shear forcing on small perturbation growth in compressible flow is investigated. In particular, flow-thermodynamic field interaction and the resulting effect on the phase-lag between applied shear and Reynolds stress are examined. Simplified linear analysis of the perturbation pressure equation reveals crucial differences between steady and unsteady shear effects. The analytical findings are validated with numerical simulations of inviscid rapid distortion theory (RDT) equations. In contrast to steadily sheared compressible flows, perturbations in the unsteady (periodic) forcing case do not experience an asymptotic growth phase. Further, the resonance growth phenomenon found in incompressible unsteady shear turbulence ismore » absent in the compressible case. Overall, the stabilizing influence of both unsteadiness and compressibility is compounded leading to suppression of all small perturbations. As a result, the underlying mechanisms are explained.« less

Rapid distortion analysis of high speed homogeneous turbulence subject to periodic shear

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bertsch, Rebecca L., E-mail: rlb@lanl.gov; Girimaji, Sharath S., E-mail: girimaji@aero.tamu.edu

2015-12-15

The effect of unsteady shear forcing on small perturbation growth in compressible flow is investigated. In particular, flow-thermodynamic field interaction and the resulting effect on the phase-lag between applied shear and Reynolds stress are examined. Simplified linear analysis of the perturbation pressure equation reveals crucial differences between steady and unsteady shear effects. The analytical findings are validated with numerical simulations of inviscid rapid distortion theory (RDT) equations. In contrast to steadily sheared compressible flows, perturbations in the unsteady (periodic) forcing case do not experience an asymptotic growth phase. Further, the resonance growth phenomenon found in incompressible unsteady shear turbulence ismore » absent in the compressible case. Overall, the stabilizing influence of both unsteadiness and compressibility is compounded leading to suppression of all small perturbations. The underlying mechanisms are explained.« less

Wall Shear Stress, Wall Pressure and Near Wall Velocity Field Relationships in a Whirling Annular Seal

NASA Technical Reports Server (NTRS)

Morrison, Gerald L.; Winslow, Robert B.; Thames, H. Davis, III

1996-01-01

The mean and phase averaged pressure and wall shear stress distributions were measured on the stator wall of a 50% eccentric annular seal which was whirling in a circular orbit at the same speed as the shaft rotation. The shear stresses were measured using flush mounted hot-film probes. Four different operating conditions were considered consisting of Reynolds numbers of 12,000 and 24,000 and Taylor numbers of 3,300 and 6,600. At each of the operating conditions the axial distribution (from Z/L = -0.2 to 1.2) of the mean pressure, shear stress magnitude, and shear stress direction on the stator wall were measured. Also measured were the phase averaged pressure and shear stress. These data were combined to calculate the force distributions along the seal length. Integration of the force distributions result in the net forces and moments generated by the pressure and shear stresses. The flow field inside the seal operating at a Reynolds number of 24,000 and a Taylor number of 6,600 has been measured using a 3-D laser Doppler anemometer system. Phase averaged wall pressure and wall shear stress are presented along with phase averaged mean velocity and turbulence kinetic energy distributions located 0.16c from the stator wall where c is the seal clearance. The relationships between the velocity, turbulence, wall pressure and wall shear stress are very complex and do not follow simple bulk flow predictions.

[Relations between plasma-erythrocyte viscosity factors and ESR].

PubMed

Cortinovis, A; Crippa, A; Crippa, M; Bosoni, T; Moratti, R

1992-09-01

The ESR is usually put in relationship: to the real density of the RBCs (erythrocytes) (difference between the RBC specific gravity and the plasma one), and to the resistance that the RBCs meet moving in a medium, which is due to the plasma viscosity and to the total external RBC surface. When the RBCs take shape of aggregates, their external surface is decreased and ESR increases. The most important plasma factor causing changes in ESR is the fibrinogen level followed by the plasma globulins and by the products arising from the tissue damage. The resistance that the RBCs meet moving in the plasma is well expressed by the measurement of the plasma-RBC viscosity considering that is inclusive of both factors that are the plasma viscosity and the external RBC surface. The plasma-RBC viscosity is the resultant of several factors: Fa = Fb - Fe - Fs - Fm, were: Fa is the resultant, Fb the attracting forces due to the proteic macromolecules, Fe the repulsing forces due the negative charges. Fs the repulsing forces due to the shear-stress, Fm the force which opposes itself against the surface tension of the aggregation; it depends on the RBC morphology and on the RBC rigidity. The ESR has been recently used like an index of the RBC aggregation. The Authors study the relationship between several hemorheological parameters and the ESR in infective and inflammatory processes.(ABSTRACT TRUNCATED AT 250 WORDS)

In vivo effects of two acidic soft drinks on shear bond strength of metal orthodontic brackets with and without resin infiltration treatment.

PubMed

Hammad, Shaza M; Enan, Enas T

2013-07-01

To evaluate the in vivo effects of two acidic soft drinks (Coca-Cola and Sprite) on the shear bond strength of metal orthodontic brackets with and without resin infiltration treatment. In addition, the enamel surface was evaluated, after debonding, using a scanning electron microscope. Sixty noncarious maxillary premolars, scheduled for extraction in 30 orthodontic patients, were used. Patients were randomly divided into two groups according to the soft drink tested (Coca-Cola or Sprite). In each group, application of resin infiltration (Icon. DMG, Hamburg, Germany) was done on one side only before bonding of brackets. Patients were told to rinse their mouth with their respective soft drink at room temperature for 5 minutes, three times a day for 3 months. Shear bond strength was tested with a universal testing machine. After shearing test, a scanning electron microscope was used to evaluate enamel erosion. Statistical analysis was performed by twoway analysis of variance followed by the least significant difference test. The Coca-Cola group without resin infiltration showed the lowest resistance to shearing forces. Scanning electron micrographs of both groups after resin application showed a significant improvement compared with results without resin use, as the enamel appeared smoother and less erosive. Pretreatment with the infiltrating resin has proved to result in a significant improvement in shear bond strength, regardless of the type of soft drink consumed.

Derivation of energy-based base shear force coefficient considering hysteretic behavior and P-delta effects

NASA Astrophysics Data System (ADS)

Ucar, Taner; Merter, Onur

2018-01-01

A modified energy-balance equation accounting for P-delta effects and hysteretic behavior of reinforced concrete members is derived. Reduced hysteretic properties of structural components due to combined stiffness and strength degradation and pinching effects, and hysteretic damping are taken into account in a simple manner by utilizing plastic energy and seismic input energy modification factors. Having a pre-selected yield mechanism, energy balance of structure in inelastic range is considered. P-delta effects are included in derived equation by adding the external work of gravity loads to the work of equivalent inertia forces and equating the total external work to the modified plastic energy. Earthquake energy input to multi degree of freedom (MDOF) system is approximated by using the modal energy-decomposition. Energy-based base shear coefficients are verified by means of both pushover analysis and nonlinear time history (NLTH) analysis of several RC frames having different number of stories. NLTH analyses of frames are performed by using the time histories of ten scaled ground motions compatible with elastic design acceleration spectrum and fulfilling duration/amplitude related requirements of Turkish Seismic Design Code. The observed correlation between energy-based base shear force coefficients and the average base shear force coefficients of NLTH analyses provides a reasonable confidence in estimation of nonlinear base shear force capacity of frames by using the derived equation.

Dynamic Shear Deformation and Failure of Ti-6Al-4V and Ti-5Al-5Mo-5V-1Cr-1Fe Alloys

PubMed Central

Chen, Pengwan

2018-01-01

To study the dynamic shear deformation and failure properties of Ti-6Al-4V (Ti-64) alloy and Ti-5Al-5Mo-5V-1Cr-1Fe (Ti-55511) alloy, a series of forced shear tests on flat hat shaped (FHS) specimens for the two investigated materials was performed using a split Hopkinson pressure bar setup. The evolution of shear deformation was monitored by an ultra-high-speed camera (Kirana-05M). Localized shear band is induced in the two investigated materials under forced shear tests. Our results indicate that severe strain localization (adiabatic shear) is accompanied by a loss in the load carrying capacity, i.e., by a sudden drop in loading. Three distinct stages can be identified using a digital image correlation technique for accurate shear strain measurement. The microstructural analysis reveals that the dynamic failure mechanisms for Ti-64 and Ti-55511 alloys within the shear band are of a cohesive and adhesive nature, respectively. PMID:29303988

Shake-table testing of a self-centering precast reinforced concrete frame with shear walls

NASA Astrophysics Data System (ADS)

Lu, Xilin; Yang, Boya; Zhao, Bin

2018-04-01

The seismic performance of a self-centering precast reinforced concrete (RC) frame with shear walls was investigated in this paper. The lateral force resistance was provided by self-centering precast RC shear walls (SPCW), which utilize a combination of unbonded prestressed post-tensioned (PT) tendons and mild steel reinforcing bars for flexural resistance across base joints. The structures concentrated deformations at the bottom joints and the unbonded PT tendons provided the self-centering restoring force. A 1/3-scale model of a five-story self-centering RC frame with shear walls was designed and tested on a shake-table under a series of bi-directional earthquake excitations with increasing intensity. The acceleration response, roof displacement, inter-story drifts, residual drifts, shear force ratios, hysteresis curves, and local behaviour of the test specimen were analysed and evaluated. The results demonstrated that seismic performance of the test specimen was satisfactory in the plane of the shear wall; however, the structure sustained inter-story drift levels up to 2.45%. Negligible residual drifts were recorded after all applied earthquake excitations. Based on the shake-table test results, it is feasible to apply and popularize a self-centering precast RC frame with shear walls as a structural system in seismic regions.

Lamin A/C deficiency reduces circulating tumor cell resistance to fluid shear stress

PubMed Central

Denais, Celine; Chan, Maxine F.; Wang, Zhexiao; Lammerding, Jan

2015-01-01

Metastasis contributes to over 90% of cancer-related deaths and is initiated when cancer cells detach from the primary tumor, invade the basement membrane, and enter the circulation as circulating tumor cells (CTCs). While metastasis is viewed as an inefficient process with most CTCs dying within the bloodstream, it is evident that some CTCs are capable of resisting hemodynamic shear forces to form secondary tumors in distant tissues. We hypothesized that nuclear lamins A and C (A/C) act as key structural components within CTCs necessary to resist destruction from elevated shear forces of the bloodstream. Herein, we show that, compared with nonmalignant epithelial cells, tumor cells are resistant to elevated fluid shear forces in vitro that mimic those within the bloodstream, as evidenced by significant decreases in cellular apoptosis and necrosis. Knockdown of lamin A/C significantly reduced tumor cell resistance to fluid shear stress, with significantly increased cell death compared with parental tumor cell and nontargeting controls. Interestingly, lamin A/C knockdown increased shear stress-induced tumor cell apoptosis, but did not significantly affect cellular necrosis. These data demonstrate that lamin A/C is an important structural component that enables tumor cell resistance to fluid shear stress-mediated death in the bloodstream, and may thus facilitate survival and hematogenous metastasis of CTCs. PMID:26447202

Quantification of the Contact Area at the Head-Stem Taper Interface of Modular Hip Prostheses.

PubMed

Witt, Florian; Gührs, Julian; Morlock, Michael M; Bishop, Nicholas E

2015-01-01

Corrosion of modular taper junctions of hip implants may be associated with clinical failure. Taper design parameters, as well as the intraoperatively applied assembly forces, have been proposed to affect corrosion. Fretting corrosion is related to relative interface shear motion and fluid ingress, which may vary with contact force and area. It was hypothesised in this study that assembly forces modify the extent and distribution of the surface contact area at the taper interface between a cobalt chrome head and titanium stem taper with a standard threaded surface profile. Local abrasion of a thin gold coating applied to the stem taper prior to assembly was used to determine the contact area after disassembly. Profilometry was then used to assess permanent deformation of the stem taper surface profile. With increasing assembly force (500 N, 2000 N, 4000 N and 8000 N) the number of stem taper surface profile ridges in contact with the head taper was found to increase (9.2±9.3%, 65.4±10.8%, 92.8±6.0% and 100%) and the overall taper area in contact was also found to increase (0.6±0.7%, 5.5±1.0%, 9.9±1.1% and 16.1±0.9%). Contact was inconsistently distributed over the length of the taper. An increase in plastic radial deformation of the surface ridges (-0.05±0.14 μm, 0.1±0.14 μm, 0.21±0.22 μm and 0.96±0.25 μm) was also observed with increasing assembly force. The limited contact of the taper surface ridges at lower assembly forces may influence corrosion rates, suggesting that the magnitude of the assembly force may affect clinical outcome. The method presented provides a simple and practical assessment of the contact area at the taper interface.

Quantification of the Contact Area at the Head-Stem Taper Interface of Modular Hip Prostheses

PubMed Central

Witt, Florian; Gührs, Julian; Morlock, Michael M.; Bishop, Nicholas E.

2015-01-01

Corrosion of modular taper junctions of hip implants may be associated with clinical failure. Taper design parameters, as well as the intraoperatively applied assembly forces, have been proposed to affect corrosion. Fretting corrosion is related to relative interface shear motion and fluid ingress, which may vary with contact force and area. It was hypothesised in this study that assembly forces modify the extent and distribution of the surface contact area at the taper interface between a cobalt chrome head and titanium stem taper with a standard threaded surface profile. Local abrasion of a thin gold coating applied to the stem taper prior to assembly was used to determine the contact area after disassembly. Profilometry was then used to assess permanent deformation of the stem taper surface profile. With increasing assembly force (500 N, 2000 N, 4000 N and 8000 N) the number of stem taper surface profile ridges in contact with the head taper was found to increase (9.2±9.3%, 65.4±10.8%, 92.8±6.0% and 100%) and the overall taper area in contact was also found to increase (0.6±0.7%, 5.5±1.0%, 9.9±1.1% and 16.1±0.9%). Contact was inconsistently distributed over the length of the taper. An increase in plastic radial deformation of the surface ridges (-0.05±0.14 μm, 0.1±0.14 μm, 0.21±0.22 μm and 0.96±0.25 μm) was also observed with increasing assembly force. The limited contact of the taper surface ridges at lower assembly forces may influence corrosion rates, suggesting that the magnitude of the assembly force may affect clinical outcome. The method presented provides a simple and practical assessment of the contact area at the taper interface. PMID:26280914

Prediction of plantar shear stress distribution by artificial intelligence methods.

PubMed

Yavuz, Metin; Ocak, Hasan; Hetherington, Vincent J; Davis, Brian L

2009-09-01

Shear forces under the human foot are thought to be responsible for various foot pathologies such as diabetic plantar ulcers and athletic blisters. Frictional shear forces might also play a role in the metatarsalgia observed among hallux valgus (HaV) and rheumatoid arthritis (RA) patients. Due to the absence of commercial devices capable of measuring shear stress distribution, a number of linear models were developed. All of these have met with limited success. This study used nonlinear methods, specifically neural network and fuzzy logic schemes, to predict the distribution of plantar shear forces based on vertical loading parameters. In total, 73 subjects were recruited; 17 had diabetic neuropathy, 14 had HaV, 9 had RA, 11 had frequent foot blisters, and 22 were healthy. A feed-forward neural network (NN) and adaptive neurofuzzy inference system (NFIS) were built. These systems were then applied to a custom-built platform, which collected plantar pressure and shear stress data as subjects walked over the device. The inputs to both models were peak pressure, peak pressure-time integral, and time to peak pressure, and the output was peak resultant shear. Root-mean-square error (RMSE) values were calculated to test the models' accuracy. RMSE/actual shear ratio varied between 0.27 and 0.40 for NN predictions. Similarly, NFIS estimations resulted in a 0.28-0.37 ratio for local peak values in all subject groups. On the other hand, error percentages for global peak shear values were found to be in the range 11.4-44.1. These results indicate that there is no direct relationship between pressure and shear magnitudes. Future research should aim to decrease error levels by introducing shear stress dependent variables into the models.

Nanoscale deicing by molecular dynamics simulation.

PubMed

Xiao, Senbo; He, Jianying; Zhang, Zhiliang

2016-08-14

Deicing is important to human activities in low-temperature circumstances, and is critical for combating the damage caused by excessive accumulation of ice. The aim of creating anti-icing materials, surfaces and applications relies on the understanding of fundamental nanoscale ice adhesion mechanics. Here in this study, we employ all-atom modeling and molecular dynamics simulation to investigate ice adhesion. We apply force to detach and shear nano-sized ice cubes for probing the determinants of atomistic adhesion mechanics, and at the same time investigate the mechanical effect of a sandwiched aqueous water layer between ice and substrates. We observe that high interfacial energy restricts ice mobility and increases both ice detaching and shearing stresses. We quantify up to a 60% decrease in ice adhesion strength by an aqueous water layer, and provide atomistic details that support previous experimental studies. Our results contribute quantitative comparison of nanoscale adhesion strength of ice on hydrophobic and hydrophilic surfaces, and supply for the first time theoretical references for understanding the mechanics at the atomistic origins of macroscale ice adhesion.

All you need is shape: Predicting shear banding in sand with LS-DEM

NASA Astrophysics Data System (ADS)

Kawamoto, Reid; Andò, Edward; Viggiani, Gioacchino; Andrade, José E.

2018-02-01

This paper presents discrete element method (DEM) simulations with experimental comparisons at multiple length scales-underscoring the crucial role of particle shape. The simulations build on technological advances in the DEM furnished by level sets (LS-DEM), which enable the mathematical representation of the surface of arbitrarily-shaped particles such as grains of sand. We show that this ability to model shape enables unprecedented capture of the mechanics of granular materials across scales ranging from macroscopic behavior to local behavior to particle behavior. Specifically, the model is able to predict the onset and evolution of shear banding in sands, replicating the most advanced high-fidelity experiments in triaxial compression equipped with sequential X-ray tomography imaging. We present comparisons of the model and experiment at an unprecedented level of quantitative agreement-building a one-to-one model where every particle in the more than 53,000-particle array has its own avatar or numerical twin. Furthermore, the boundary conditions of the experiment are faithfully captured by modeling the membrane effect as well as the platen displacement and tilting. The results show a computational tool that can give insight into the physics and mechanics of granular materials undergoing shear deformation and failure, with computational times comparable to those of the experiment. One quantitative measure that is extracted from the LS-DEM simulations that is currently not available experimentally is the evolution of three dimensional force chains inside and outside of the shear band. We show that the rotations on the force chains are correlated to the rotations in stress principal directions.

Proximity sounding analysis for derechos and supercells: an assessment of similarities and differences

NASA Astrophysics Data System (ADS)

Doswell, Charles A.; Evans, Jeffry S.

Proximity soundings (within 2 h and 167 km) of derechos (long-lived, widespread damaging convective windstorms) and supercells have been obtained. More than 65 derechos, accompanied by 115 proximity soundings, are identified during the years 1983 to 1993. The derechos have been divided into categories according to the synoptic situation: strong forcing (SF), weak forcing (WF), and "hybrid" cases (which are neither weakly nor strongly forced). Nearly 100 supercell proximity soundings have been found for the period 1998 to 2001, subdivided into nontornadic and tornadic supercells; tornadic supercells were further subdivided into those producing significant (>F1 rating) tornadoes and weak tornadoes (F0-F1 rating). WF derecho situations typically are characterized by warm, moist soundings with large convective available potential instability (CAPE) and relatively weak vertical wind shear. SF derechos usually have stronger wind shears, and cooler and less moist soundings with lower CAPE than the weakly forced cases. Most derechos exhibit strong storm-relative inflow at low levels. In WF derechos, this is usually the result of rapid convective system movement, whereas in SF derechos, storm-relative inflow at low levels is heavily influenced by relatively strong low-level windspeeds. "Hybrid" cases collectively are similar to an average of the SF and WF cases. Supercells occur in environments that are not all that dissimilar from those that produce SF derechos. It appears that some parameter combining instability and deep layer shear, such as the Energy-Helicity Index (EHI), can help discriminate between tornadic and nontornadic supercell situations. Soundings with significant tornadoes (F2 and greater) typically show high 0-1 km relative humidities, and strong 0-1 km shear. Results suggest it may not be easy to forecast the mode of severe thunderstorm activity (i.e., derecho versus supercell) on any particular day, given conditions that favor severe thunderstorm activity in general. It is possible that the convective initiation mechanism is an important factor, with linear initiation favoring derechos, whereas nonlinear forcing might favor supercells. Upper-level storm-relative flow in supercells tends to be rear-to-front, whereas for derechos, storm-relative flow tends to be front-to-rear through a deep surface-based layer. However, knowing the storm-relative hodograph requires knowledge of storm motion, which can be a challenge to predict. These results generally imply that probabilistic forecasts of convective mode could be a successful strategy.

Evaluation of a conditioning method to improve core-veneer bond strength of zirconia restorations.

PubMed

Teng, Jili; Wang, Hang; Liao, Yunmao; Liang, Xing

2012-06-01

The high strength and fracture toughness of zirconia have supported its extensive application in esthetic dentistry. However, the fracturing of veneering porcelains remains one of the primary causes of failure. The purpose of this study was to evaluate, with shear bond strength testing, the effect of a simple and novel surface conditioning method on the core-veneer bond strength of a zirconia ceramic system. The shear bond strength of a zirconia core ceramic to the corresponding veneering porcelain was tested by the Schmitz-Schulmeyer method. Thirty zirconia core specimens (10 × 5 × 5 mm) were layered with a veneering porcelain (5 × 3 × 3 mm). Three different surface conditioning methods were evaluated: polishing with up to 1200 grit silicon carbide paper under water cooling, airborne-particle abrasion with 110 μm alumina particles, and modification with zirconia powder coating before sintering. A metal ceramic system was used as a control group. All specimens were subjected to shear force in a universal testing machine at a crosshead speed of 0.5 mm/min. The shear bond strength values were analyzed with 1-way ANOVA and Tukey's post hoc pairwise comparisons (α=.05). The fractured specimens were examined with a scanning electron microscope to observe the failure mode. The mean (SD) shear bond strength values in MPa were 47.02 (6.4) for modified zirconia, 36.66 (8.6) for polished zirconia, 39.14 (6.5) for airborne-particle-abraded zirconia, and 46.12 (7.1) for the control group. The mean bond strength of the control (P=.028) and modified zirconia groups (P=.014) was significantly higher than that of the polished zirconia group. The airborne-particle-abraded group was not significantly different from any other group. Scanning electron microscopy evaluation showed that cohesive fracture in the veneering porcelain was the predominant failure mode of modified zirconia, while the other groups principally fractured at the interface. Modifying the zirconia surface with powder coating could significantly increase the shear bond strength of zirconia to veneering porcelain. Copyright © 2012 The Editorial Council of the Journal of Prosthetic Dentistry. Published by Mosby, Inc. All rights reserved.

Microbial responses to microgravity and other low-shear environments.

PubMed

Nickerson, Cheryl A; Ott, C Mark; Wilson, James W; Ramamurthy, Rajee; Pierson, Duane L

2004-06-01

Microbial adaptation to environmental stimuli is essential for survival. While several of these stimuli have been studied in detail, recent studies have demonstrated an important role for a novel environmental parameter in which microgravity and the low fluid shear dynamics associated with microgravity globally regulate microbial gene expression, physiology, and pathogenesis. In addition to analyzing fundamental questions about microbial responses to spaceflight, these studies have demonstrated important applications for microbial responses to a ground-based, low-shear stress environment similar to that encountered during spaceflight. Moreover, the low-shear growth environment sensed by microbes during microgravity of spaceflight and during ground-based microgravity analogue culture is relevant to those encountered during their natural life cycles on Earth. While no mechanism has been clearly defined to explain how the mechanical force of fluid shear transmits intracellular signals to microbial cells at the molecular level, the fact that cross talk exists between microbial signal transduction systems holds intriguing possibilities that future studies might reveal common mechanotransduction themes between these systems and those used to sense and respond to low-shear stress and changes in gravitation forces. The study of microbial mechanotransduction may identify common conserved mechanisms used by cells to perceive changes in mechanical and/or physical forces, and it has the potential to provide valuable insight for understanding mechanosensing mechanisms in higher organisms. This review summarizes recent and future research trends aimed at understanding the dynamic effects of changes in the mechanical forces that occur in microgravity and other low-shear environments on a wide variety of important microbial parameters.

Microbial Responses to Microgravity and Other Low-Shear Environments

NASA Technical Reports Server (NTRS)

Nickerson, Cheryl A.; Ott, C. Mark; Wilson, James W.; Ramamurthy, Rajee; Pierson, Duane L.

2004-01-01

Microbial adaptation to environmental stimuli is essential for survival. While several of these stimuli have been studied in detail, recent studies have demonstrated an important role for a novel environmental parameter in which microgravity and the low fluid shear dynamics associated with microgravity globally regulate microbial gene expression, physiology, and pathogenesis. In addition to analyzing fundamental questions about microbial responses to spaceflight, these studies have demonstrated important applications for microbial responses to a ground-based, low-shear stress environment similar to that encountered during spaceflight. Moreover, the low-shear growth environment sensed by microbes during microgravity of spaceflight and during ground-based microgravity analogue culture is relevant to those encountered during their natural life cycles on Earth. While no mechanism has been clearly defined to explain how the mechanical force of fluid shear transmits intracellular signals to microbial cells at the molecular level, the fact that cross talk exists between microbial signal transduction systems holds intriguing possibilities that future studies might reveal common mechanotransduction themes between these systems and those used to sense and respond to low-shear stress and changes in gravitation forces. The study of microbial mechanotransduction may identify common conserved mechanisms used by cells to perceive changes in mechanical and/or physical forces, and it has the potential to provide valuable insight for understanding mechanosensing mechanisms in higher organisms. This review summarizes recent and future research trends aimed at understanding the dynamic effects of changes in the mechanical forces that occur in microgravity and other low-shear environments on a wide variety of important microbial parameters.

Surface temperatures and glassy state investigations in tribology

NASA Technical Reports Server (NTRS)

Bair, S.; Winer, W. O.

1980-01-01

Measurements were made of the limiting shear stress for two naphthenic oils of differing molecular weight and three blends of the lower molecular weight oil and polymers of differing molecular weight. All reached the same limiting shear stress for the same temperature and pressure; although the polymer solutions reduced the limiting shear stress by about fifteen percent. A falling body viscometer was constructed to operate to 230 C and to 0.6 GPa and another was constructed to extend the pressure range to 1.1 GPa. A concentrated contact simulator was developed which allows recording of the traction force while the slide-roll ratio is continuously varied and the rolling speed is maintained essentially constant. Measurement of lubricant minimum film thickness of elliptical EHD contacts of various aspect ratios were made by optical interferometry. The experimental data were thirty percent greater than that predicted by the Hamrock and Dowson model. Preliminary development of the application of a scanning infrared radiation system to a tribological system was completed.

Shear Elasticity and Shear Viscosity Imaging in Soft Tissue

NASA Astrophysics Data System (ADS)

Yang, Yiqun

In this thesis, a new approach is introduced that provides estimates of shear elasticity and shear viscosity using time-domain measurements of shear waves in viscoelastic media. Simulations of shear wave particle displacements induced by an acoustic radiation force are accelerated significantly by a GPU. The acoustic radiation force is first calculated using the fast near field method (FNM) and the angular spectrum approach (ASA). The shear waves induced by the acoustic radiation force are then simulated in elastic and viscoelastic media using Green's functions. A parallel algorithm is developed to perform these calculations on a GPU, where the shear wave particle displacements at different observation points are calculated in parallel. The resulting speed increase enables rapid evaluation of shear waves at discrete points, in 2D planes, and for push beams with different spatial samplings and for different values of the f-number (f/#). The results of these simulations show that push beams with smaller f/# require a higher spatial sampling rate. The significant amount of acceleration achieved by this approach suggests that shear wave simulations with the Green's function approach are ideally suited for high-performance GPUs. Shear wave elasticity imaging determines the mechanical parameters of soft tissue by analyzing measured shear waves induced by an acoustic radiation force. To estimate the shear elasticity value, the widely used time-of-flight method calculates the correlation between shear wave particle velocities at adjacent lateral observation points. Although this method provides accurate estimates of the shear elasticity in purely elastic media, our experience suggests that the time-of-flight (TOF) method consistently overestimates the shear elasticity values in viscoelastic media because the combined effects of diffraction, attenuation, and dispersion are not considered. To address this problem, we have developed an approach that directly accounts for all of these effects when estimating the shear elasticity. This new approach simulates shear wave particle velocities using a Green's function-based approach for the Voigt model, where the shear elasticity and viscosity values are estimated using an optimization-based approach that compares measured shear wave particle velocities with simulated shear wave particle velocities in the time-domain. The results are evaluated on a point-by-point basis to generate images. There is good agreement between the simulated and measured shear wave particle velocities, where the new approach yields much better images of the shear elasticity and shear viscosity than the TOF method. The new estimation approach is accelerated with an approximate viscoelastic Green's function model that is evaluated with shear wave data obtained from in vivo human livers. Instead of calculating shear waves with combinations of different shear elasticities and shear viscosities, shear waves are calculated with different shear elasticities on the GPU and then convolved with a viscous loss model, which accelerates the calculation dramatically. The shear elasticity and shear viscosity values are then estimated using an optimization-based approach by minimizing the difference between measured and simulated shear wave particle velocities. Shear elasticity and shear viscosity images are generated at every spatial point in a two-dimensional (2D) field-of-view (FOV). The new approach is applied to measured shear wave data obtained from in vivo human livers, and the results show that this new approach successfully generates shear elasticity and shear viscosity images from this data. The results also indicate that the shear elasticity values estimated with this approach are significantly smaller than the values estimated with the conventional TOF method and that the new approach demonstrates more consistent values for these estimates compared with the TOF method. This experience suggests that the new method is an effective approach for estimating the shear elasticity and the shear viscosity in liver and in other soft tissue.

Irreversible particle motion in surfactant-laden interfaces due to pressure-dependent surface viscosity

NASA Astrophysics Data System (ADS)

Manikantan, Harishankar; Squires, Todd M.

2017-09-01

The surface shear viscosity of an insoluble surfactant monolayer often depends strongly on its surface pressure. Here, we show that a particle moving within a bounded monolayer breaks the kinematic reversibility of low-Reynolds-number flows. The Lorentz reciprocal theorem allows such irreversibilities to be computed without solving the full nonlinear equations, giving the leading-order contribution of surface pressure-dependent surface viscosity. In particular, we show that a disc translating or rotating near an interfacial boundary experiences a force in the direction perpendicular to that boundary. In unbounded monolayers, coupled modes of motion can also lead to non-intuitive trajectories, which we illustrate using an interfacial analogue of the Magnus effect. This perturbative approach can be extended to more complex geometries, and to two-dimensional suspensions more generally.

Compressive and shear hip joint contact forces are affected by pediatric obesity during walking

PubMed Central

Lerner, Zachary F.; Browning, Raymond C.

2016-01-01

Obese children exhibit altered gait mechanics compared to healthy-weight children and have an increased prevalence of hip pain and pathology. This study sought to determine the relationships between body mass and compressive and shear hip joint contact forces during walking. Kinematic and kinetic data were collected during treadmill walking at 1 m•s−1 in 10 obese and 10 healthy-weight 8–12 year-olds. We estimated body composition, segment masses, lower-extremity alignment, and femoral neck angle via radiographic images, created personalized musculoskeletal models in OpenSim, and computed muscle forces and hip joint contact forces. Hip extension at mid-stance was 9° less, on average, in the obese children (p<0.001). Hip abduction, knee flexion, and body-weight normalized peak hip moments were similar between groups. Normalized to body-weight, peak contact forces were similar at the first peak and slightly lower at the second peak between the obese and healthy-weight participants. Total body mass explained a greater proportion of contact force variance compared to lean body mass in the compressive (r2=0.89) and vertical shear (perpendicular to the physis acting superior-to-inferior) (r2=0.84) directions; lean body mass explained a greater proportion in the posterior shear direction (r2=0.54). Stance-average contact forces in the compressive and vertical shear directions increased by 41 N and 48 N, respectively, for every kilogram of body mass. Age explained less than 27% of the hip loading variance. No effect of sex was found. The proportionality between hip loads and body-weight may be implicated in an obese child’s increased risk of hip pain and pathology. PMID:27040390

Compressive and shear hip joint contact forces are affected by pediatric obesity during walking.

PubMed

Lerner, Zachary F; Browning, Raymond C

2016-06-14

Obese children exhibit altered gait mechanics compared to healthy-weight children and have an increased prevalence of hip pain and pathology. This study sought to determine the relationships between body mass and compressive and shear hip joint contact forces during walking. Kinematic and kinetic data were collected during treadmill walking at 1ms(-1) in 10 obese and 10 healthy-weight 8-12 year-olds. We estimated body composition, segment masses, lower-extremity alignment, and femoral neck angle via radiographic images, created personalized musculoskeletal models in OpenSim, and computed muscle forces and hip joint contact forces. Hip extension at mid-stance was 9° less, on average, in the obese children (p<0.001). Hip abduction, knee flexion, and body-weight normalized peak hip moments were similar between groups. Normalized to body-weight, peak contact forces were similar at the first peak and slightly lower at the second peak between the obese and healthy-weight participants. Total body mass explained a greater proportion of contact force variance compared to lean body mass in the compressive (r(2)=0.89) and vertical shear (perpendicular to the physis acting superior-to-inferior) (r(2)=0.84) directions; lean body mass explained a greater proportion in the posterior shear direction (r(2)=0.54). Stance-average contact forces in the compressive and vertical shear directions increased by 41N and 48N, respectively, for every kilogram of body mass. Age explained less than 27% of the hip loading variance. No effect of sex was found. The proportionality between hip loads and body-weight may be implicated in an obese child׳s increased risk of hip pain and pathology. Published by Elsevier Ltd.

Beating of grafted chains induced by active Brownian particles

NASA Astrophysics Data System (ADS)

Yang, Qiu-song; Fan, Qing-wei; Shen, Zhuang-lin; Xia, Yi-qi; Tian, Wen-de; Chen, Kang

2018-06-01

We study the interplay between active Brownian particles (ABPs) and a "hairy" surface in two-dimensional geometry. We find that the increase of propelling force leads to and enhances inhomogeneous accumulation of ABPs inside the brush region. Oscillation of chain bundles (beating like cilia) is found in company with the formation and disassembly of a dynamic cluster of ABPs at large propelling forces. Meanwhile chains are stretched and pushed down due to the effective shear force by ABPs. The decrease of the average brush thickness with propelling force reflects the growth of the beating amplitude of chain bundles. Furthermore, the beating phenomenon is investigated in a simple single-chain system. We find that the chain swings regularly with a major oscillatory period, which increases with chain length and decreases with the increase of propelling force. We build a theory to describe the phenomenon and the predictions on the relationship between the period and amplitude for various chain lengths, and propelling forces agree very well with simulation data.

A diffraction correction for storage and loss moduli imaging using radiation force based elastography.

PubMed

Budelli, Eliana; Brum, Javier; Bernal, Miguel; Deffieux, Thomas; Tanter, Mickaël; Lema, Patricia; Negreira, Carlos; Gennisson, Jean-Luc

2017-01-07

Noninvasive evaluation of the rheological behavior of soft tissues may provide an important diagnosis tool. Nowadays, available commercial ultrasound systems only provide shear elasticity estimation by shear wave speed assessment under the hypothesis of a purely elastic model. However, to fully characterize the rheological behavior of tissues, given by its storage (G') and loss (G″) moduli, it is necessary to estimate both: shear wave speed and shear wave attenuation. Most elastography techniques use the acoustic radiation force to generate shear waves. For this type of source the shear waves are not plane and a diffraction correction is needed to properly estimate the shear wave attenuation. The use of a cylindrical wave approximation to evaluate diffraction has been proposed by other authors before. Here the validity of such approximation is numerically and experimentally revisited. Then, it is used to generate images of G' and G″ in heterogeneous viscoelastic mediums. A simulation algorithm based on the anisotropic and viscoelastic Green's function was used to establish the validity of the cylindrical approximation. Moreover, two experiments were carried out: a transient elastography experiment where plane shear waves were generated using a vibrating plate and a SSI experiment that uses the acoustic radiation force to generate shear waves. For both experiments the shear wave propagation was followed with an ultrafast ultrasound scanner. Then, the shear wave velocity and shear wave attenuation were recovered from the phase and amplitude decay versus distance respectively. In the SSI experiment the cylindrical approximation was applied to correct attenuation due to diffraction effects. The numerical and experimental results validate the use of a cylindrical correction to assess shear wave attenuation. Finally, by applying the cylindrical correction G' and G″ images were generated in heterogeneous phantoms and a preliminary in vivo feasibility study was carried out in the human liver.

A diffraction correction for storage and loss moduli imaging using radiation force based elastography

NASA Astrophysics Data System (ADS)

Budelli, Eliana; Brum, Javier; Bernal, Miguel; Deffieux, Thomas; Tanter, Mickaël; Lema, Patricia; Negreira, Carlos; Gennisson, Jean-Luc

2017-01-01

Noninvasive evaluation of the rheological behavior of soft tissues may provide an important diagnosis tool. Nowadays, available commercial ultrasound systems only provide shear elasticity estimation by shear wave speed assessment under the hypothesis of a purely elastic model. However, to fully characterize the rheological behavior of tissues, given by its storage (G‧) and loss (G″) moduli, it is necessary to estimate both: shear wave speed and shear wave attenuation. Most elastography techniques use the acoustic radiation force to generate shear waves. For this type of source the shear waves are not plane and a diffraction correction is needed to properly estimate the shear wave attenuation. The use of a cylindrical wave approximation to evaluate diffraction has been proposed by other authors before. Here the validity of such approximation is numerically and experimentally revisited. Then, it is used to generate images of G‧ and G″ in heterogeneous viscoelastic mediums. A simulation algorithm based on the anisotropic and viscoelastic Green’s function was used to establish the validity of the cylindrical approximation. Moreover, two experiments were carried out: a transient elastography experiment where plane shear waves were generated using a vibrating plate and a SSI experiment that uses the acoustic radiation force to generate shear waves. For both experiments the shear wave propagation was followed with an ultrafast ultrasound scanner. Then, the shear wave velocity and shear wave attenuation were recovered from the phase and amplitude decay versus distance respectively. In the SSI experiment the cylindrical approximation was applied to correct attenuation due to diffraction effects. The numerical and experimental results validate the use of a cylindrical correction to assess shear wave attenuation. Finally, by applying the cylindrical correction G‧ and G″ images were generated in heterogeneous phantoms and a preliminary in vivo feasibility study was carried out in the human liver.

Bacterial adherence to graft tissues in static and flow conditions.

PubMed

Veloso, Tiago Rafael; Claes, Jorien; Van Kerckhoven, Soetkin; Ditkowski, Bartosz; Hurtado-Aguilar, Luis G; Jockenhoevel, Stefan; Mela, Petra; Jashari, Ramadan; Gewillig, Marc; Hoylaerts, Marc F; Meyns, Bart; Heying, Ruth

2018-01-01

Various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. Valve replacement carries an increased risk of infective endocarditis (IE). Recent observations have increased awareness of the risk of IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. This study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE. Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus sanguinis to bovine pericardium (BP) patch, bovine jugular vein (BJV), and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components. In general, similar bacteria numbers were recovered from CH and BJV tissue surfaces for all strains, especially in flow conditions. Static bacterial adhesion to the CH wall was lower for S sanguinis adhesion (P < .05 vs BP patch). Adhesion to the BJV wall, CH wall, and leaflet was decreased for S epidermidis in static conditions (P < .05 vs BP patch). Bacterial adhesion under shear stress indicated similar bacterial adhesion to all tissues, except for lower adhesion to the BJV wall after S sanguinis incubation. Microscopic analysis showed the importance of matrix component exposure for bacterial adherence to CH. Our data provide evidence that the surface composition of BJV and CH tissues themselves, bacterial surface proteins, and shear forces per se are not the prime determinants of bacterial adherence. Copyright © 2017 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.

Shear-induced inflation of coronal magnetic fields

NASA Technical Reports Server (NTRS)

Klimchuk, James A.

1989-01-01

Using numerical models of force-free magnetic fields, the shearing of footprints in arcade geometries leading to an inflation of the coronal magnetic field was examined. For each of the shear profiles considered, all of the field lines become elevated compared with the potential field. This includes cases where the shear is concentrated well away from the arcade axis, such that B(sub z), the component of field parallel to the axis, increases outward to produce an inward B(sub z)squared/8 pi magnetic pressure gradient force. These results contrast with an earlier claim, shown to be incorrect, that field lines can sometimes become depressed as a result of shear. It is conjectured that an inflation of the entire field will always result from the shearing of simple arcade configurations. These results have implications for prominence formation, the interplanetary magnetic flux, and possibly also coronal holes.

Shear-induced inflation of coronal magnetic fields

NASA Technical Reports Server (NTRS)

Klimchuk, James A.

1990-01-01

Using numerical models of force-free magnetic fields, the shearing of footprints in arcade geometries leading to an inflation of the coronal magnetic field was examined. For each of the shear profiles considered, all of the field lines become elevated compared with the potential field. This includes cases where the shear is concentrated well away from the arcade axis, such that B(sub z), the component of field parallel to the axis, increases outward to produce an inward B(sub z) squared/8 pi magnetic pressure gradient force. These results contrast with an earlier claim, shown to be incorrect, that field lines can sometimes become depressed as a result of shear. It is conjectured that an inflation of the entire field will always result from the shearing of simple arcade configurations. These results have implications for prominence formation, the interplanetary magnetic flux, and possibly also coronal holes.

Effects of electrode bevel angle on argon arc properties and weld shape

NASA Astrophysics Data System (ADS)

Dong, W. C.; Lu, S. P.; Li, D. Z.; Y Li, Y.

2012-07-01

A numerical modeling of coupled welding arc with weld pool is established using FLUENT software for moving shielded GTA welding to systematically investigate the effects of electrode bevel angle on the argon arc properties as well as the weld shape on SUS304 stainless steel. The calculated results show that the argon arc is constricted and the peak values of heat flux and shear stress on the weld pool decrease with increasing electrode bevel angle, while the radial distribution of heat flux and shear stress varying slightly. The weld shape is controlled by the pool flow patterns driving by the surface tension, gas shear stress, electromagnetic force and buoyancy. The Marangoni convection induced by surface tension plays an important role on weld shapes. All the weld shapes are wide and shallow with low weld metal oxygen content, while the narrow and deep weld shapes form under high weld metal oxygen content, which is related with the oxygen concentration in the shielding gas. The weld depth/width (D/W) ratio increases with increasing electrode bevel angle for high weld metal oxygen content and is not sensitive to the electrode bevel angle under low weld metal oxygen content. The calculated results for the weld shape, weld size and weld D/W ratio agree well with the experimental ones.

Method for measuring surface shear stress magnitude and direction using liquid crystal coatings

NASA Technical Reports Server (NTRS)

Reda, Daniel C. (Inventor)

1995-01-01

A method is provided for determining surface shear magnitude and direction at every point on a surface. The surface is covered with a shear stress sensitive liquid crystal coating and illuminated by white light from a normal direction. A video camera is positioned at an oblique angle above the surface to observe the color of the liquid crystal at that angle. The shear magnitude and direction are derived from the color information. A method of calibrating the device is also provided.

DNS of Laminar-Turbulent Transition in Swept-Wing Boundary Layers

NASA Technical Reports Server (NTRS)

Duan, L.; Choudhari, M.; Li, F.

2014-01-01

Direct numerical simulation (DNS) is performed to examine laminar to turbulent transition due to high-frequency secondary instability of stationary crossflow vortices in a subsonic swept-wing boundary layer for a realistic natural-laminar-flow airfoil configuration. The secondary instability is introduced via inflow forcing and the mode selected for forcing corresponds to the most amplified secondary instability mode that, in this case, derives a majority of its growth from energy production mechanisms associated with the wall-normal shear of the stationary basic state. An inlet boundary condition is carefully designed to allow for accurate injection of instability wave modes and minimize acoustic reflections at numerical boundaries. Nonlinear parabolized stability equation (PSE) predictions compare well with the DNS in terms of modal amplitudes and modal shape during the strongly nonlinear phase of the secondary instability mode. During the transition process, the skin friction coefficient rises rather rapidly and the wall-shear distribution shows a sawtooth pattern that is analogous to the previously documented surface flow visualizations of transition due to stationary crossflow instability. Fully turbulent features are observed in the downstream region of the flow.

Effect of Different Connection Modes on Bolt Structural Properties of TC4 Alloy in Selective Laser Melting

NASA Astrophysics Data System (ADS)

Li, Xiaodan; Huang, Shuangjun; Xu, Liang; Hui, Li; Zhou, Song

2017-12-01

The bolt structural properties of selective laser melted (SLM) samples produced from TC4 powder metal has been investigated. Two different connection molds relative to single lap joint and bilateral lap joint as well as two different state of surface quality were considered. Samples and test procedures were designed in accordance with HB 5143 and HB 5287 standard. The results show that there is a strong influence of connection molds on the dynamic behavior of SLM produced TC4. The mechanical properties of bilateral lap joint are better than those of the single lap joint. Meanwhile the fatigue performance of the bilateral lap joint is much stronger than that of the single lap joint which it is a symmetrical structure of the two-shear test on both sides of the force evenly, while the single lap joint is a single shear sample of the uneven force. There are two kinds of fracture form most of which are broken in the first row of screw and a small part in the middle of the connecting plate.

The Microtremor H/V Spectral Ratio: The Physical Basis of the Diffuse Field Assumption

NASA Astrophysics Data System (ADS)

Sanchez-Sesma, F. J.

2016-12-01

The microtremor H/V spectral ratio (MHVSR) is popular to obtain the dominant frequency at a site. Despite the success of MHVSR some controversy arose regarding its physical basis. One approach is the Diffuse Field Assumption, DFA. It is then assumed that noise diffuse features come from multiple scattering within the medium. According to theory, the average of the autocorrelation is proportional to directional energy density (DED) and to the imaginary part of the Green's function for same source and receiver. Then, the square of MHVSR is a ratio of DEDs which, in a horizontally layered system, is 2xImG11/ImG33, where ImG11 and ImG33 are the imaginary parts of Green's functions for horizontal and vertical components. This has physical implications that emerge from the duality DED-force, implicit in the DFA. Consider a surface force at a half-space. The radiated energy is carried away by various wave types and the proportions of each one are precisely the fractions of the energy densities of a diffuse elastic wave field at the free surface. Thus, some properties of applied forces are also characteristics of DEDs. For example, consider a Poisson solid. For a normal point load, 67 per cent of energy is carried away by Rayleigh waves. For the tangential case, it is less well known that, 77 per cent of energy goes as shear waves. In a full space, 92 per cent of the energy is emitted as shear waves. The horizontal DED at the half-space surface implies significant emission of down-going shear waves that explains the curious stair-like resonance spectrum of ImG11. Both ImG11 and ImG33 grow linearly versus frequency and this represents wave emission. For a layered medium, besides wave emission, the ensuing variations correspond to reflected waves. For high frequencies, ImG33 depends on the properties of the top layer. Reflected body waves are very small and Rayleigh waves behave in the top layer as in a kind of mini half-space. From HVSR one can invert the velocity model using the DFA. It is possible to compute efficiently the imaginary part of the Green's functions from the integrals along the radial wavenumber k. This can be made using either the Bouchon DWN method or the Cauchy residue theorem to get the pole contributions of Rayleigh and Love surface waves in the k complex plane. This allows separating the contributions of each wave type.

Effect of electrical stimulation and cooking temperature on the within-sample variation of cooking loss and shear force of lamb.

PubMed

Lewis, P K; Babiker, S A

1983-01-01

Electrical stimulation decreased the shear force and increased the cooking loss in seven paired lamb Longissimus dorsi (LD) muscles. This treatment did not have any effect on the within-sample variation. Cooking in 55°, 65° and 75°C water baths for 90 min caused a linear increase in the cooking loss and shear force. There was no stimulation-cooking temperature interaction observed. Cooking temperature also had no effect on the within-sample variation. A possible explanation as to why electrical stimulation did not affect the within-sample variation is given. Copyright © 1983. Published by Elsevier Ltd.

Adjustable shear stress erosion and transport flume

DOEpatents

Roberts, Jesse D.; Jepsen, Richard A.

2002-01-01

A method and apparatus for measuring the total erosion rate and downstream transport of suspended and bedload sediments using an adjustable shear stress erosion and transport (ASSET) flume with a variable-depth sediment core sample. Water is forced past a variable-depth sediment core sample in a closed channel, eroding sediments, and introducing suspended and bedload sediments into the flow stream. The core sample is continuously pushed into the flow stream, while keeping the surface level with the bottom of the channel. Eroded bedload sediments are transported downstream and then gravitationally separated from the flow stream into one or more quiescent traps. The captured bedload sediments (particles and aggregates) are weighed and compared to the total mass of sediment eroded, and also to the concentration of sediments suspended in the flow stream.

Approximate Stokes Drift Profiles in Deep Water

NASA Astrophysics Data System (ADS)

Breivik, Øyvind; Janssen, Peter A. E. M.; Bidlot, Jean-Raymond

2014-09-01

A deep-water approximation to the Stokes drift velocity profile is explored as an alternative to the monochromatic profile. The alternative profile investigated relies on the same two quantities required for the monochromatic profile, viz the Stokes transport and the surface Stokes drift velocity. Comparisons with parametric spectra and profiles under wave spectra from the ERA-Interim reanalysis and buoy observations reveal much better agreement than the monochromatic profile even for complex sea states. That the profile gives a closer match and a more correct shear has implications for ocean circulation models since the Coriolis-Stokes force depends on the magnitude and direction of the Stokes drift profile and Langmuir turbulence parameterizations depend sensitively on the shear of the profile. The alternative profile comes at no added numerical cost compared to the monochromatic profile.

Mars boundary layer simulations - Comparison with Viking lander and entry observations

NASA Technical Reports Server (NTRS)

Haberle, R. M.; Houben, H. C.

1991-01-01

Diurnal variations of wind and temperature in the lower Martian atmosphere are simulated with a boundary layer model that includes radiative heating in a dusty CO2 atmosphere, turbulence generated by convection and/or shear stresses, a surface heat budget, and time varying pressure forces due to sloping terrain. Model results for early northern summer are compared with Viking lander observations to determine the model's strengths and weaknesses, and suitability as an engineering model.

Biaxial and Shear Testing Apparatus with Force Controls

DTIC Science & Technology

2006-03-30

materials as the test specimen. (2) Description of the Prior Art [0004] It is known in the art that pressurized fabric tubes ; pressure-stabilized beams...apparatus is that these roller pins prevent any torsional load from reaching the test specimen. [0010] In Ward et al., (United States Patent No. 5,279,166...loading a specimen through pressurizing the inside surface of a cylinder is disclosed. A thin-wall tube specimen is biaxially tested for stress analysis

Rheology of granular materials composed of crushable particles.

PubMed

Nguyen, Duc-Hanh; Azéma, Émilien; Sornay, Philippe; Radjaï, Farhang

2018-04-11

We investigate sheared granular materials composed of crushable particles by means of contact dynamics simulations and the bonded-cell model for particle breakage. Each particle is paved by irregular cells interacting via cohesive forces. In each simulation, the ratio of the internal cohesion of particles to the confining pressure, the relative cohesion, is kept constant and the packing is subjected to biaxial shearing. The particles can break into two or more fragments when the internal cohesive forces are overcome by the action of compressive force chains between particles. The particle size distribution evolves during shear as the particles continue to break. We find that the breakage process is highly inhomogeneous both in the fragment sizes and their locations inside the packing. In particular, a number of large particles never break whereas a large number of particles are fully shattered. As a result, the packing keeps the memory of its initial particle size distribution, whereas a power-law distribution is observed for particles of intermediate size due to consecutive fragmentation events whereby the memory of the initial state is lost. Due to growing polydispersity, dense shear bands are formed inside the packings and the usual dilatant behavior is reduced or cancelled. Hence, the stress-strain curve no longer passes through a peak stress, and a progressive monotonic evolution towards a pseudo-steady state is observed instead. We find that the crushing rate is controlled by the confining pressure. We also show that the shear strength of the packing is well expressed in terms of contact anisotropies and force anisotropies. The force anisotropy increases while the contact orientation anisotropy declines for increasing internal cohesion of the particles. These two effects compensate each other so that the shear strength is nearly independent of the internal cohesion of particles.

Cancer Cell Glycocalyx Mediates Mechanostransduction and Flow-Regulated Invasion

PubMed Central

Qazi, Henry; Palomino, Rocio; Shi, Zhong-Dong; Munn, Lance L.; Tarbell, John M.

2014-01-01

Mammalian cells are covered by a surface proteoglycan (glycocalyx) layer, and it is known that blood vessel-lining endothelial cells use the glycocalyx to sense and transduce the shearing forces of blood flow into intracellular signals. Tumor cells in vivo are exposed to forces from interstitial fluid flow that may affect metastatic potential but are not reproduced by most in vitro cell motility assays. We hypothesized that glycocalyx-mediated mechanotransduction of interstitial flow shear stress is an un-recognized factor that can significantly enhance metastatic cell motility and play a role in augmentation of invasion. Involvement of MMP levels, cell adhesion molecules (CD44, α3 integrin), and glycocalyx components (heparan sulfate and hyaluronan) were investigated in a cell/collagen gel suspension model designed to mimic the interstitial flow microenvironment. Physiologic levels of flow upregulated MMP levels and enhanced the motility of metastatic cells. Blocking the flow-enhanced expression of MMP actvity or adhesion molecules (CD44 and integrins) resulted in blocking the flow-enhanced migratory activity. The presence of a glycocalyx-like layer was verified around tumor cells, and the degradation of this layer by hyaluronidase and heparinase blocked the flow-regulated invasion. This study shows for the first time that interstitial flow enhancement of metastatic cell motility can be mediated by the cell surface glycocalyx – a potential target for therapeutics. PMID:24077103

Use of gelatin gels as a reference material for performance evaluation of meat shear force measurements

USDA-ARS?s Scientific Manuscript database

Establishing standards for meat tenderness based on Warner-Bratzler shear force (WBSF) is complicated by the lack of methods for certifying WBSF testing among texture systems or laboratories. The objective of this study was to determine the suitability of using gelatin gels as a reference material ...

Genome Wide Scan for Loci influencing Warner Bratzler Shear Force in Five Bos taurus Breeds

USDA-ARS?s Scientific Manuscript database

Genetic tests for beef tenderness are currently limited to single nucleotide polymorphisms (SNPs) within µ-calpain (CAPN1) and calpastatin (CAST) and explain little of the phenotypic variation in Warner-Bratzler shear force (WBSF). We performed a genome-wide association study for WBSF by genotyping...

Time-frequency analyses of fluid-solid interaction under sinusoidal translational shear deformation of the viscoelastic rat cerebrum

NASA Astrophysics Data System (ADS)

Leahy, Lauren N.; Haslach, Henry W.

2018-02-01

During normal extracellular fluid (ECF) flow in the brain glymphatic system or during pathological flow induced by trauma resulting from impacts and blast waves, ECF-solid matter interactions result from sinusoidal shear waves in the brain and cranial arterial tissue, both heterogeneous biological tissues with high fluid content. The flow in the glymphatic system is known to be forced by pulsations of the cranial arteries at about 1 Hz. The experimental shear stress response to sinusoidal translational shear deformation at 1 Hz and 25% strain amplitude and either 0% or 33% compression is compared for rat cerebrum and bovine aortic tissue. Time-frequency analyses aim to correlate the shear stress signal frequency components over time with the behavior of brain tissue constituents to identify the physical source of the shear nonlinear viscoelastic response. Discrete fast Fourier transformation analysis and the novel application to the shear stress signal of harmonic wavelet decomposition both show significant 1 Hz and 3 Hz components. The 3 Hz component in brain tissue, whose magnitude is much larger than in aortic tissue, may result from interstitial fluid induced drag forces. The harmonic wavelet decomposition locates 3 Hz harmonics whose magnitudes decrease on subsequent cycles perhaps because of bond breaking that results in easier fluid movement. Both tissues exhibit transient shear stress softening similar to the Mullins effect in rubber. The form of a new mathematical model for the drag force produced by ECF-solid matter interactions captures the third harmonic seen experimentally.

Calculation Method of Lateral Strengths and Ductility Factors of Constructions with Shear Walls of Different Ductility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yamaguchi, Nobuyoshi; Nakao, Masato; Murakami, Masahide

2008-07-08

For seismic design, ductility-related force modification factors are named R factor in Uniform Building Code of U.S, q factor in Euro Code 8 and Ds (inverse of R) factor in Japanese Building Code. These ductility-related force modification factors for each type of shear elements are appeared in those codes. Some constructions use various types of shear walls that have different ductility, especially for their retrofit or re-strengthening. In these cases, engineers puzzle the decision of force modification factors of the constructions. Solving this problem, new method to calculate lateral strengths of stories for simple shear wall systems is proposed andmore » named 'Stiffness--Potential Energy Addition Method' in this paper. This method uses two design lateral strengths for each type of shear walls in damage limit state and safety limit state. Two lateral strengths of stories in both limit states are calculated from these two design lateral strengths for each type of shear walls in both limit states. Calculated strengths have the same quality as values obtained by strength addition method using many steps of load-deformation data of shear walls. The new method to calculate ductility factors is also proposed in this paper. This method is based on the new method to calculate lateral strengths of stories. This method can solve the problem to obtain ductility factors of stories with shear walls of different ductility.« less

Application of a soft computing technique in predicting the percentage of shear force carried by walls in a rectangular channel with non-homogeneous roughness.

PubMed

Khozani, Zohreh Sheikh; Bonakdari, Hossein; Zaji, Amir Hossein

2016-01-01

Two new soft computing models, namely genetic programming (GP) and genetic artificial algorithm (GAA) neural network (a combination of modified genetic algorithm and artificial neural network methods) were developed in order to predict the percentage of shear force in a rectangular channel with non-homogeneous roughness. The ability of these methods to estimate the percentage of shear force was investigated. Moreover, the independent parameters' effectiveness in predicting the percentage of shear force was determined using sensitivity analysis. According to the results, the GP model demonstrated superior performance to the GAA model. A comparison was also made between the GP program determined as the best model and five equations obtained in prior research. The GP model with the lowest error values (root mean square error ((RMSE) of 0.0515) had the best function compared with the other equations presented for rough and smooth channels as well as smooth ducts. The equation proposed for rectangular channels with rough boundaries (RMSE of 0.0642) outperformed the prior equations for smooth boundaries.

Study of toluene rotary fluid management device and shear flow condenser performance for a space-based organic Rankine power system

NASA Technical Reports Server (NTRS)

Havens, Vance; Ragaller, Dana

1988-01-01

Management of two-phase fluid and control of the heat transfer process in microgravity is a technical challenge that must be addressed for an orbital Organic Rankine Cycle (ORC) application. A test program was performed in 1-g that satisfactorily demonstrated the two-phase management capability of the rotating fluid management device (RFMD) and shear-flow condenser. Operational tests of the RFMD and shear flow condenser in adverse gravity orientations, confirmed that the centrifugal forces in the RFMD and the shear forces in the condenser were capable of overcoming gravity forces. In a microgravity environment, these same forces would not have to compete against gravity and would therefore be dominant. The specific test program covered the required operating range of the Space Station Solar Dynamic Rankine Cycle power system. Review of the test data verified that: fluid was pumped from the RFMD in all attitudes; subcooled states in the condenser were achieved; condensate was pushed uphill against gravity; and noncondensible gases were swept through the condenser.

Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy

NASA Astrophysics Data System (ADS)

Nguyen, Son N.; Sontag, Ryan L.; Carson, James P.; Corley, Richard A.; Ansong, Charles; Laskin, Julia

2018-02-01

Constant mode ambient mass spectrometry imaging (MSI) of tissue sections with high lateral resolution of better than 10 μm was performed by combining shear force microscopy with nanospray desorption electrospray ionization (nano-DESI). Shear force microscopy enabled precise control of the distance between the sample and nano-DESI probe during MSI experiments and provided information on sample topography. Proof-of-concept experiments were performed using lung and brain tissue sections representing spongy and dense tissues, respectively. Topography images obtained using shear force microscopy were comparable to the results obtained using contact profilometry over the same region of the tissue section. Variations in tissue height were found to be dependent on the tissue type and were in the range of 0-5 μm for lung tissue and 0-3 μm for brain tissue sections. Ion images of phospholipids obtained in this study are in good agreement with literature data. Normalization of nano-DESI MSI images to the signal of the internal standard added to the extraction solvent allowed us to construct high-resolution ion images free of matrix effects.

Pre rigor processing, ageing and freezing on tenderness and colour stability of lamb loins.

PubMed

Kim, Yuan H Brad; Luc, Genevieve; Rosenvold, Katja

2013-10-01

Forty eight lamb carcasses with temperature and pH monitored were obtained from two commercial plants. At 24h post mortem both loins (M. longissimus) from each carcass were randomly allocated to a) unaged frozen at -18°C, (b) aged at -1.5°C for 2weeks before freezing, (c) aged for 3 weeks before freezing and (d) aged for 9 weeks without freezing. Shear force, colour stability and proteolysis were analyzed. Carcasses with a slower temperature and more rapid pH decline had more calpain autolysis, slightly higher shear force and less colour stable compared to that counterpart in general (P<0.05). However, the shear force values of the loins were all acceptable (<6 kgF) regardless of different pre rigor processing and ageing/freezing treatments. Furthermore, the loins aged for 2 weeks-then-frozen/thawed had a similar shear force to the loins aged only for 9 weeks suggesting that ageing-then-freezing would result in equivalent tenderness compared to aged only loins for the long-term storage. Copyright © 2013 Elsevier Ltd. All rights reserved.

Analysis of wall shear stress around a competitive swimmer using 3D Navier-Stokes equations in CFD.

PubMed

Popa, C V; Zaidi, H; Arfaoui, A; Polidori, G; Taiar, R; Fohanno, S

2011-01-01

This paper deals with the flow dynamics around a competitive swimmer during underwater glide phases occurring at the start and at every turn. The influence of the head position, namely lifted up, aligned and lowered, on the wall shear stress and the static pressure distributions is analyzed. The problem is considered as 3D and in steady hydrodynamic state. Three velocities (1.4 m/s, 2.2 m/s and 3.1 m/s) that correspond to inter-regional, national and international swimming levels are studied. The flow around the swimmer is assumed turbulent. The Reynolds-averaged Navier-Stokes (RANS) equations are solved with the standard k-ω turbulent model by using the CFD (computational fluid dynamics) numerical method based on a volume control approach. Numerical simulations are carried out with the ANSYS FLUENT® CFD code. The results show that the wall shear stress increases with the velocity and consequently the drag force opposing the movement of the swimmer increases as well. Also, high wall shear stresses are observed in the areas where the body shape, globally rigid in form, presents complex surface geometries such as the head, shoulders, buttocks, heel and chest.

Energy buildup in sheared force-free magnetic fields

NASA Technical Reports Server (NTRS)

Wolfson, Richard; Low, Boon C.

1992-01-01

Photospheric displacement of the footpoints of solar magnetic field lines results in shearing and twisting of the field, and consequently in the buildup of electric currents and magnetic free energy in the corona. The sudden release of this free energy may be the origin of eruptive events like coronal mass ejections, prominence eruptions, and flares. An important question is whether such an energy release may be accompanied by the opening of magnetic field lines that were previously closed, for such open field lines can provide a route for matter frozen into the field to escape the sun altogether. This paper presents the results of numerical calculations showing that opening of the magnetic field is permitted energetically, in that it is possible to build up more free energy in a sheared, closed, force-free magnetic field than is in a related magnetic configuration having both closed and open field lines. Whether or not the closed force-free field attains enough energy to become partially open depends on the form of the shear profile; the results presented compare the energy buildup for different shear profiles. Implications for solar activity are discussed briefly.

Stress transfer in microdroplet tensile test: PVC-coated and uncoated Kevlar-29 single fiber

NASA Astrophysics Data System (ADS)

Zhenkun, Lei; Quan, Wang; Yilan, Kang; Wei, Qiu; Xuemin, Pan

2010-11-01

The single fiber/microdroplet tensile test is applied for evaluating the interfacial mechanics between a fiber and a resin substrate. It is used to investigate the influence of a polymer coating on a Kevlar-29 fiber surface, specifically the stress transfer between the fiber and epoxy resin in a microdroplet. Unlike usual tests, this new test ensures a symmetrical axial stress on the embedded fiber and reduces the stress singularity that appears at the embedded fiber entry. Using a homemade loading device, symmetrical tensile tests are performed on a Kevlar-29 fiber with or without polyvinylchloride (PVC) coating, the surface of which is in contact with two epoxy resin microdroplets during curing. Raman spectra on the embedded fiber are recorded by micro-Raman Spectroscopy under different strain levels. Then they are transformed to the distributions of fiber axis stress based on the relationship between stress and Raman shift. The Raman results reveal that the fiber axial stresses increase with the applied loads, and the antisymmetric interfacial shear stresses, obtained by a straightforward balance of shear-to-axial forces argument, lead to the appearance of shear stress concentrations at a distance to the embedded fiber entry. The load is transferred from the outer fiber to the embedded fiber in the epoxy microdroplet. As is observed by scanning electronic microscopy (SEM), the existence of a flexible polymer coating on the fiber surface reduces the stress transfer efficiency.

Fourier Transform Surface Plasmon Resonance of Nanodisks Embedded in Magnetic Nanorods.

PubMed

Jung, Insub; Ih, Seongkeun; Yoo, Haneul; Hong, Seunghun; Park, Sungho

2018-03-14

In this study, we demonstrate the synthesis and application of magnetic plasmonic gyro-nanodisks (GNDs) for Fourier transform surface plasmon resonance based biodetection. Plasmonically active and magnetically responsive gyro-nanodisks were synthesized using electrochemical methods with anodized aluminum templates. Due to the unique properties of GNDs (magnetic responsiveness and surface plasmon bands), periodic extinction signals were generated under an external rotating magnetic field, which is, in turn, converted into frequency domains using Fourier transformation. After the binding of a target on GNDs, an increase in the shear force causes a shift in the frequency domain, which allows us to investigate biodetection for HA1 (the influenza virus). Most importantly, by modulating the number and the location of plasmonic nanodisks (a method for controlling the hydrodynamic forces by rationally designing the nanomaterial architecture), we achieved enhanced biodetection sensitivity. We expect that our results will contribute to improved sensing module performance, as well as a better understanding of dynamic nanoparticle systems, by harnessing the perturbed periodic fluctuation of surface plasmon bands under the modulated magnetic field.

Buoyancy Driven Shear Flows of Bubble Suspensions

NASA Technical Reports Server (NTRS)

Koch, D. L.; Hill, R. J.; Chellppannair, T.; Zenit, R.; Zenit, R.; Spelt, P. D. M.

1999-01-01

In this work the gas volume fraction and the root-mean-squared fluid velocity are measured in buoyancy driven shear flows of bubble suspensions in a tall, inclined, rectangular channel. The experiments are performed under conditions where We << 1a nd Re >> 1, for which comparisons are made with kinetic theory and numerical simulations. Here Re = gamma(a(exp 2)/nu is the Reynolds number and We = rho(gamma(exp 2))a(exp 3)/sigma is the Weber number; gamma is the shear rate, a is the bubble radius, nu is the kinematic viscosity of the liquid, rho is the density of the liquid, and sigma is the surface tension of the gas/liquid interface. Kang et al. calculated the bubble phase pressure and velocity variance of sheared bubble suspensions under conditions where the bubbles are spherical and the liquid phase velocity field can be approximated using potential flow theory, i.e. We= 0 and Re >> 1. Such conditions can be achieved in an experiment using gas bubbles, with a radius of O(0.5mm), in water. The theory requires that there be no average relative motion of the gas and liquid phases, hence the motivation for an experimental program in microgravity. The necessity of performing preliminary, Earth based experiments, however, requires performing experiments where the gas phase rises in the liquid, which significantly complicates the comparison of experiments with theory. Rather than comparing experimental results with theory for a uniform, homogeneous shear flow, experiments can be compared directly with solutions of the averaged equations of motion for bubble suspensions. This requires accounting for the significant lift force acting on the gas phase when the bubbles rise parallel to the average velocity of the sheared suspension. Shear flows can be produced in which the bubble phase pressure gradient, arising from shear induced collisions amongst the bubbles, balances a body force (centrifugal or gravitational) on the gas phase. A steady, non-uniform gas volume fraction can be measured, from which the bubble phase pressure gradient can be obtained and compared to theory and numerical simulations. The presence of bounding walls further complicates the experiments, since the detailed interactions of the bubbles with bounding walls is not well understood, especially in the presence of gravity, where the momentum and energy exchange depends on the inclination of the wall.

Influence of Freestream and Forced Disturbances on the Shear Layers of a Square Prism

NASA Astrophysics Data System (ADS)

Lander, Daniel Chapman

Flow around the square prism, an archetypal bluff body, has applications in all areas of fluid mechanics: vibration, mixing, combustion and noise production to name a few. It also has distinct importance to wind loading on architectural and industrial structures such as tall buildings, bridges, and towers. The von-Karman (VK) vortex street is a major reason for its significance: a flow phenomenon which has received intense scrutiny from scientific and engineering communities for more than 100 years! However, the characteristics of the shear layers separating from the sharp edges, essential to the vortex shedding, have received comparatively little attention. This is surprising considering the Kelvin-Helmholtz (KH) instability of shear layers produce the first signatures of turbulence in the wake. Furthermore, the shear layers are conduits for the passage of vorticity between the boundary layer and the turbulent wake. Many details of their structure and role in the shedding process remain unexplored. This dissertation aims to address this deficiency. Specifically, this project considered the influence of three variables on the characteristics of the transition-to-turbulence in the square prism shear layers. These are: (1) Reynolds number; (2) freestream disturbances and (3) forced disturbances. In each case, the dynamics of the shear layer-wake interaction were considered. Particle image velocimetry and constant temperature anemometry measurements were used to document the shear layer during inception and evolution as it passes into the wake. With increasing Reynolds number, ReD = UinfinityD/nu, in the range 16,700-148,000, the transition-to-turbulence in the initially laminar shear layer moves toward separation. A coordinate system local to the time-averaged shear layer axis was used such that the tangent and normal velocities, turbulent stresses and gradient quantities could be obtained for the curved shear layer. Characteristic frequencies, lengths and transition points of the KH instability were documented and shown to exhibit features distinct from the plane mixing layer. The evolution of the integrated turbulent kinetic energy was documented and a linear region of growth was associated with the amplification of the KH instability. A scaling relationship of the Kelvin-Helmholtz to von-Karman frequencies was established for the square prism shear layer. ƒKH/ƒ VK was shown to be a power-law function of Re D, with differing characteristics to the much more studied circular cylinder. Increasing ReD up to ˜ 70,000 bolsters the Reynolds stresses in the shear layers as they enter the wake, shortening the wake formation length, LF. The shear layer diffusion length, LD was quantified and the Gerrard-Product, LF x LD, was introduced to account for constant St D in the presence of the reduced LF as function of ReD. A freestream disturbance condition with intensity □ u¯¯ 2¯ / U infinity = 0.065 and longitudinal integral length scale, Lxu = 0.33 was considered for the case of ReD = 50,000. Disturbances were introduced by means of small circular cylinder placed upstream of the stagnation streamline. The disturbance moved the time-averaged position of the shear layer towards the body but did not substantially alter the growth rate of its width. The "normal" transition-to-turbulence pathway, via laminar vortex formation and subsequent pairing of vortices in the initial stages of the shear layer was shown to be highly sensitive to external disturbances. The disturbance interrupted the typical transition pathway and was associated with a Bypass-transition mechanism, which subsequently increased the likelihood of intermittent shear layer reattachment on the downstream surface of the body. Triple decomposition was used to study the random and coherent components of the VK structures in the wake. Data indicated a narrowing and lengthening of the wake, which was accompanied by a rise in base pressure and a reduction in time-averaged drag. The unsteady coherent vorticity field revealed a streamwise elongation of the VK vortex structures, which complemented the time-averaged wake lengthening. It appears that the influence of freestream disturbances, in particular, by their stochastic nature, is to suppress the formation of the coherent structures in the shear layer. Forced disturbances imposed on the shear layers at the leading edges of the square prism were considered at ReD=16,700 for excitation frequencies ƒe = ƒ KH, ƒVK and 0. The response of the shear layer to forcing at steady and ƒVK frequencies had little impact on the time-averaged position or growth.

Obesity is associated with higher absolute tibiofemoral contact and muscle forces during gait with and without knee osteoarthritis.

PubMed

Harding, Graeme T; Dunbar, Michael J; Hubley-Kozey, Cheryl L; Stanish, William D; Astephen Wilson, Janie L

2016-01-01

Obesity is an important risk factor for knee osteoarthritis initiation and progression. However, it is unclear how obesity may directly affect the mechanical loading environment of the knee joint, initiating or progressing joint degeneration. The objective of this study was to investigate the interacting role of obesity and moderate knee osteoarthritis presence on tibiofemoral contact forces and muscle forces within the knee joint during walking gait. Three-dimensional gait analysis was performed on 80 asymptomatic participants and 115 individuals diagnosed with moderate knee osteoarthritis. Each group was divided into three body mass index categories: healthy weight (body mass index<25), overweight (25≤body mass index≤30), and obese (body mass index>30). Tibiofemoral anterior-posterior shear and compressive forces, as well as quadriceps, hamstrings and gastrocnemius muscle forces, were estimated based on a sagittal plane contact force model. Peak contact and muscle forces during gait were compared between groups, as well as the interaction between disease presence and body mass index category, using a two-factor analysis of variance. There were significant osteoarthritis effects in peak shear, gastrocnemius and quadriceps forces only when they were normalized to body mass, and there were significant BMI effects in peak shear, compression, gastrocnemius and hamstrings forces only in absolute, non-normalized forces. There was a significant interaction effect in peak quadriceps muscle forces, with higher forces in overweight and obese groups compared to asymptomatic healthy weight participants. Body mass index was associated with higher absolute tibiofemoral compression and shear forces as well as posterior muscle forces during gait, regardless of moderate osteoarthritis presence or absence. The differences found may contribute to accelerated joint damage with obesity, but with the osteoarthritic knees less able to accommodate the high loads. Copyright © 2015 Elsevier Ltd. All rights reserved.

A national audit of retail lamb loin quality in Australia.

PubMed

Safari, E; Channon, H A; Hopkins, D L; Hall, D G; van de Ven, R

2002-07-01

A retail audit of lamb loin tenderness was conducted over a 12-month period to determine the variation in tenderness of Australian lamb. Tenderness was objectively measured using Warner-Bratzler (WB) shear force. Muscle pH and cooking loss were determined on all samples and colour was measured on a sub-sample of loins. A total of 909 midloins from retail butcher shops and supermarkets located in four Australian capital cities (Sydney, Canberra, Melbourne, and Perth) were evaluated at four sampling times (December 1997 and March, June, and October 1998). Overall, 20.3% of all midloins purchased had a WB shear force value above the threshold level of 5 kg. Generic samples from Melbourne butcher shops were similar for WB shear force on average to the generic samples from Canberra and Sydney, whereas those from Melbourne supermarkets had significantly (P<0.001) higher WB shear force and were in line with generic samples from Perth. In both Canberra and Perth, alliance (branded) lamb had a greater WB shear force (P<0.05) than generic lamb. No relationship was found between price per kg and shear force (r=0.02) for loins purchased in Sydney (n=220). Price per kg differed between months (P<0.001) and suburbs (P<0.001), but not between retail butcher shops and supermarkets. Of the midloins tested, 10.3% had a pH above the critical point of 5.8. Midloins from the December 1997 sampling had a lower pH (P<0.01) than those sampled at other months. Those sampled in Melbourne and Perth had a similar mean pH, which were lower (P<0.001) than Canberra and Sydney samples. The findings from this quality audit suggest that there is room to improve the tenderness of Australian lamb sold in the domestic market. A lamb eating quality assurance system, based on set protocols, is one approach that is currently being investigated in Australia to ensure the supply of consistently high eating quality lamb to consumers.

Advances in active control and optimization in turbulence

NASA Astrophysics Data System (ADS)

Freeman, Aaron Paul

The main objective of this research is to explore the effectiveness of pulsed plasma actuators for turbulence control. In particular, a pulsed plasma actuator is used in this research to implement active control, in the form of a localized body force, over turbulent separated shear layers. Applications of tins research include controlling the formation and distribution of large scale turbulent structures and optimizing turbulence-aberrated laser propagation. This research is primarily experimental, with the motivation for the work derived from theoretical analysis of a turbulent shear layer. The experimental work is considered within two primary flow regimes, compressible and incompressible. For both cases, a turbulent shear layer is generated and then forced with plasma which is introduced periodically at frequencies ranging between 1.0 kHz and 25.0 kHz. The Reynolds numbers, based on visual thickness, of the compressible and incompressible flows investigated in this research are 6.0 106 and 8.0 104 respectively. Experimental results for the compressible case, based on Shack-Hartmann profiling of turbulence-aberrated laser wavefronts, for laser propagation through forced and unforced shear flows show reductions in the laser aberrations of up to 27.5% with a pulsing frequency of 5.0 kHz as well as increases of up to 16.9% with a pulsing frequency of 1.0 kHz. Other pulsing frequencies within the specified range were experimental analyzed and found to exhibit little or no significant change in the laser aberrations compared to the unforced case. The direct results from the Shack-Hartmann wavefront sensor are used to calculate the power spectra of the recorded Optical Path Difference profiles to verify the correlation between large aero-optical aberrations and propagation through large turbulent structures. Shadowgraph imaging of the compressible flow field was conducted to visually demonstrate the same. The experimental procedure for the incompressible shear layer involves imaging the flow field using fog-Mie scattering. The analysis for the resulting incompressible shear layer images include investigations of the distribution of large scale structures and the associated effects that periodic forcing has on the shear layer relating to mixing enhancement and scalar geometry. The effects of periodic forcing on mixing will be determined based on the scalar probability density function and the scalar power spectrum. In addition, the geometry of the scalar interfaces will be examined in terms of the generalized fractal dimension to determine the effects that periodic forcing has on the scale dependency of self-similarity within the flow field. Results from the experiments for the incompressible shear layer show that mixing can be increased by up to 8.4% as determined based on increases within the intermediate scalar probability density function and decreased by as much as 30.8% at forcing frequencies of 25.0 kHz and 1.0 kHz respectively. Additionally, this research shows that the extent of the range of scales of geometrical self-similarity of iso-concentration interfaces extracted from the flow images can be increased by up to 75.0% or reduced by as much as 75.0% depending on the forcing frequency applied. These results show that aero-optical interactions in a compressible shear layer as well as both mixing and the interfacial geometry in incompressible shear layers can be substantially modified by the periodic forcing.

Shear shedding of drops and the use of superhydrophobic surfaces in microgravity: PFC and ground based results

NASA Astrophysics Data System (ADS)

Milne, Andrew; Amirfazli, Alidad

In free fall, the absence of gravity poses many challenges for fluid handling systems. One such example of this is condensers. On earth, the condensed liquid is removed from the tilted condenser plate by gravity forced shedding. In microgravity, proposed solutions include the use of surfaces with gradients in wettability [1], the use of electrowetting [2], and shearing airflow [3]. In this talk, shear shedding results for a variety of surface (hydrophilic to superhydrophobic (extremely water repelling)) will be presented. Surface science and aerodynamics are used to reveal fundamental parameters controlling incipient motion for drops exposed to shearing airflow. It is found that wetting parameters such as contact angle and surface tension are very influential in determining the minimum required air velocity for drop shedding. Based on experimental results for drops of water and hexadecane (0.5-100 l) on PMMA, Teflon, and a superhydrophobic aluminum surface, an exponential function is proposed that relates the critical air velocity for shedding to the ratio of drop base length to projected area. The results for the water systems can be collapsed to a self similar curve by normalization, which also explains results from other researchers. Since shedding from superhydrophobic surfaces (SHS) is seen to be easier compared to other surfaces, the behaviour of SHS is also probed in this talk. SHS have space-based applications to shedding, self cleaning, anti-icing (spacecraft launch/re-entry), anti-fouling, fluid actuation, and decreased fluid friction. The mechanism for SHS is understood to be the existence of an air layer between large portions of the drop and solid. The first concrete visual evidence of this was gained performing a parabolic flight experiment with the ESA. Results of this experi-ment will be discussed, showing the extreme water repelling potential of SHS in microgravity, and demonstrating how the wetting behaviours seen (partial penetration, transition of wetting states, unpredicted contact angle behaviour) affect models of superhydrophobicity and the use of SHS to both space and Earth based applications. 1) Darhuber, A. A.; Troian, S. M. Annual Review of Fluid Mechanics 2005, 425-455. 2) Berthier, J.; Dubois, P.; Clementz, P.; Claustre, P.; Peponnet, C.; Fouillet, Y. Sensors and Actuators A: Physical 2007, 134, 471-479. 3) Milne, A. J. B.; Amirfazli, A. Langmuir 2009, 25, 14155-14164.

[Biofilm Formation by the Nonflagellated flhB1 Mutant of Azospirillum brasilense Sp245].

PubMed

Shelud'ko, A V; Filip'echeva, Yu A; Shumiliva, E M; Khlebtsov, B N; Burov, A M; Petrova, L P; Katsy, E I

2015-01-01

Azospirillum brasilense Sp245 with mixed flagellation are able to form biofilms on various surfaces. A nonflagellated mutant of this strain with inactivated chromosomal copy of the flhB gene (flhB1) was shown to exhibit specific traits at the later stages of biofilm formation on a hydrophilic (glass) surface. Mature biofilms of the flhB1::Omegon-Km mutant Sp245.1063 were considerably thinner than those of the parent strain Sp245. The biofilms of the mutant were more susceptible to the forces of hydrodynamic shear. A. brasilense Sp245 cells in biofilms were not found to possess lateral flagella. Cells with polar flagella were, however, revealed by atomic force microscopy of mature native biofilms of strain Sp245. Preservation of a polar flagellum (probably nonmotile) on the cells of A. brasilense Sp245 may enhance the biofilm stability.

Numerical Simulation of Parachute Inflation Process by IB Method

NASA Astrophysics Data System (ADS)

Miyoshi, Masaya; Mori, Koichi; Nakamura, Yoshiaki

In the present study the deformation and motion of a parachute in the process of inflation are simulated by applying the immersed boundary technique in a fluid-structure coupling solver. It was found from simulated results that the canopy is first inflated in the normal direction to the uniform flow (in the lateral direction), and then its apex is pulled by a vortex ring generated near the canopy's outer surface due to its negative pressure. After the end of this inflation process, the canopy moves in the tangential direction to the spherical surface, the center of which is located at the payload location. This motion is caused by the breakup of an initial axisymmetric vortex, where many vortices are generated from the shear layer. The predicted maximum parachute opening force is twice as large as the payload force in the steady state, which is in good agreement with experiment.

Generation of internal solitary waves by frontally forced intrusions in geophysical flows.

PubMed

Bourgault, Daniel; Galbraith, Peter S; Chavanne, Cédric

2016-12-06

Internal solitary waves are hump-shaped, large-amplitude waves that are physically analogous to surface waves except that they propagate within the fluid, along density steps that typically characterize the layered vertical structure of lakes, oceans and the atmosphere. As do surface waves, internal solitary waves may overturn and break, and the process is thought to provide a globally significant source of turbulent mixing and energy dissipation. Although commonly observed in geophysical fluids, the origins of internal solitary waves remain unclear. Here we report a rarely observed natural case of the birth of internal solitary waves from a frontally forced interfacial gravity current intruding into a two-layer and vertically sheared background environment. The results of the analysis carried out suggest that fronts may represent additional and unexpected sources of internal solitary waves in regions of lakes, oceans and atmospheres that are dynamically similar to the situation examined here in the Saguenay Fjord, Canada.

Effect of contact time and force on monocyte adhesion to vascular endothelium.

PubMed Central

Rinker, K D; Prabhakar, V; Truskey, G A

2001-01-01

In this study we examined whether monocytic cell attachment to vascular endothelium was affected by elevating shear stress at a constant shear rate. Contact time, which is inversely related to the shear rate, was fixed and viscosity elevated with dextran to increase the shear stress (and hence the net force on the cell) independently of shear rate. At a fixed contact time, tethering frequencies increased, rolling velocities decreased, and median arrest durations increased with increasing shear stress. Rolling and short arrests (< 0.2 s) were well fit by a single exponential consistent with adhesion via the formation of a single additional bond. The cell dissociation constant, k(off), increased when the shear stress was elevated at constant shear rate. Firmly adherent cells arresting for at least 0.2 s were well fit by a stochastic model involving dissociation from multiple bonds. Therefore, at a fixed contact time and increasing shear stress, bonds formed more frequently for rolling cells resulting in more short arrests, and more bonds formed for firmly arresting cells resulting in longer arrest durations. Possible mechanisms for this increased adhesion include greater monocyte deformation and/or more frequent penetration of microvilli through steric and charge barriers. PMID:11259286

Laboratory studies of frictional sliding and the implications of precursory seismicity

NASA Astrophysics Data System (ADS)

Selvadurai, Paul A.

The dynamic transition from slow to rapid sliding along a frictional interface is of interest to geophysicists, engineers and scientists alike. In our direct shear experiment, we simulated a pre-existing frictional fault similar to those occurring naturally in the Earth. The laboratory study reported here has incorporated appropriate sensors that can detect foreshock events on the fringe of a nucleation zone prior to a gross fault rupture (main shock). During loading we observed foreshocks sequences as slip transitioned from slow to rapid sliding. These laboratory-induced foreshocks showed similar acoustic characteristics and spatio-temporal evolution as those detected in nature. Through direct observation (video camera), foreshocks were found to be the rapid, localized (millimeter length scale) failure of highly stresses asperities formed along the interface. The interface was created by the meshing of two rough polymethyl methacrylate (PMMA) bodies in a direct shear configuration. A carefully calibrated pressure sensitive film was used to map the contact junctions (asperities) throughout the interface at a range of applied normal loads Fn. Foreshocks were found to coalesce in a region of the fault that exhibited a more dense distribution of asperities (referred to as the seismogenic region). Microscopy of the interface in the seismogenic region displayed a variety of surface roughness at various length scales. This may have been introduced from the surface preparation techniques use to create a mature interface. The mature interface consisted of 'flat-topped' asperity regions with separating sharp valleys. The 'flat-topped' sections spanned millimetric length scales and were considerably flatter (nanometric roughness) that the roughness exhibited at longer length scales (tens of millimeters). We believe that the smoother, 'flat-topped' sections were responsible for the individual asperity formation (determining their size and strength), whereas the longer length scale roughness influenced the asperity-asperity interaction during the nucleation phase. Asperities in the seismogenic region where shown to exist close enough to each other so that elastic communication (through the off-fault material) could not be neglected. Prior to gross fault rupture (i.e. mainshock), we measured the propagation of a slow nucleating rupture into the relatively 'locked', seimsogenic region of the fault. Slow slip dynamics were captured using slip sensors placed along the fault that measured a non-uniform slip profile leading up to failure. We found that the propagation of the slow rupture into the locked region was dependent on the normal force Fn. Higher Fn was found to slow the propagation of shear rupture into the locked region. Within the relatively 'locked' region, a noticeable increase in size and a more compact spatial-temporal distribution of foreshocks were measured when Fn was increased. In order to develop an understanding of the relationship between Fn and the resistance of the fault to slow rupture, a quasi-static finite element (FE) model was developed. The model used distributions of asperities measured directly from the pressure sensitive film in a small section of the interface where foreshocks coalesced; specifically, the region where the slowly propagating slip front encountered the more dense distribution of asperities. A single asperity was modeled and followed the Cattaneo partial slip asperity solution. As the shear force increased along the fault, the asperities in this model were able to accommodate tangential slip by entering a partial sliding regime; the central contact of the asperities remained adhered while sliding accumulated along its periphery. Partial slip on the asperity propagated inwards as the shear force was incrementally increased. A further increase in the shear force caused the asperity to enter a full sliding condition. Increasing confining loads caused increased stiffness and increased capacity to store potential shear strain energy -- a possible measure of the 'degree of coupling' between the fault surfaces. Physics from the numerical model followed the qualitative observations made using photometry of asperities along the interface, which visualized asperities in the 'locked' region -- larger asperities remained stuck throughout the loading cycle and the light transmitted through individual asperities decreased from the periphery as shear loads increased. The numerical partial slip, quantified by the potential energy stored by the asperity, increased relative to the normal pressure p. Asperity-asperity interactions were modeled along the interface using a quasi-static analysis. Progression of slip into the asperity field was increasingly inhibited as the normal confining force Fn was increased. The computational model provided an explanation as to why an increased confining force Fn could result in an increased resistance to slow rupture as well as an increased potential for larger foreshocks within the resistive, relatively 'locked' section of a fault. This study lays the foundation for more innovative laboratory work that could potentially improve the phenomenological models currently used to estimate earthquake hazard. (Abstract shortened by UMI.).

The efficacy of three objective systems for identifying beef cuts that can be guaranteed tender.

PubMed

Wheeler, T L; Vote, D; Leheska, J M; Shackelford, S D; Belk, K E; Wulf, D M; Gwartney, B L; Koohmaraie, M

2002-12-01

The objective of this study was to determine the accuracy of three objective systems (prototype BeefCam, colorimeter, and slice shear force) for identifying guaranteed tender beef. In Phase I, 308 carcasses (105 Top Choice, 101 Low Choice, and 102 Select) from two commercial plants were tested. In Phase II, 400 carcasses (200 rolled USDA Select and 200 rolled USDA Choice) from one commercial plant were tested. The three systems were evaluated based on progressive certification of the longissimus as "tender" in 10% increments (the best 10, 20, 30%, etc., certified as "tender" by each technology; 100% certification would mean no sorting for tenderness). In Phase I, the error (percentage of carcasses certified as tender that had Warner-Bratzler shear force of > or = 5 kg at 14 d postmortem) for 100% certification using all carcasses was 14.1%. All certification levels up to 80% (slice shear force) and up to 70% (colorimeter) had less error (P < 0.05) than 100% certification. Errors in all levels of certification by prototype BeefCam (13.8 to 9.7%) were not different (P > 0.05) from 100% certification. In Phase I, the error for 100% certification for USDA Select carcasses was 30.7%. For Select carcasses, all slice shear force certification levels up to 60% (0 to 14.8%) had less error (P < 0.05) than 100% certification. For Select carcasses, errors in all levels of certification by colorimeter (20.0 to 29.6%) and by BeefCam (27.5 to 31.4%) were not different (P > 0.05) from 100% certification. In Phase II, the error for 100% certification for all carcasses was 9.3%. For all levels of slice shear force certification less than 90% (for all carcasses) or less than 80% (Select carcasses), errors in tenderness certification were less than (P < 0.05) for 100% certification. In Phase II, for all carcasses or Select carcasses, colorimeter and prototype BeefCam certifications did not significantly reduce errors (P > 0.05) compared to 100% certification. Thus, the direct measure of tenderness provided by slice shear force results in more accurate identification of "tender" beef carcasses than either of the indirect technologies, prototype BeefCam, or colorimeter, particularly for USDA Select carcasses. As tested in this study, slice shear force, but not the prototype BeefCam or colorimeter systems, accurately identified "tender" beef.

Indentation of a rigid sphere into an elastic substrate with surface tension and adhesion

PubMed Central

Hui, Chung-Yuen; Liu, Tianshu; Salez, Thomas; Raphael, Elie; Jagota, Anand

2015-01-01

The surface tension of compliant materials such as gels provides resistance to deformation in addition to and sometimes surpassing that owing to elasticity. This paper studies how surface tension changes the contact mechanics of a small hard sphere indenting a soft elastic substrate. Previous studies have examined the special case where the external load is zero, so contact is driven by adhesion alone. Here, we tackle the much more complicated problem where, in addition to adhesion, deformation is driven by an indentation force. We present an exact solution based on small strain theory. The relation between indentation force (displacement) and contact radius is found to depend on a single dimensionless parameter: ω=σ(μR)−2/3((9π/4)Wad)−1/3, where σ and μ are the surface tension and shear modulus of the substrate, R is the sphere radius and Wad is the interfacial work of adhesion. Our theory reduces to the Johnson–Kendall–Roberts (JKR) theory and Young–Dupre equation in the limits of small and large ω, respectively, and compares well with existing experimental data. Our results show that, although surface tension can significantly affect the indentation force, the magnitude of the pull-off load in the partial wetting liquid-like limit is reduced only by one-third compared with the JKR limit and the pull-off behaviour is completely determined by ω. PMID:25792953

The Effect of Tow Shearing on Reinforcement Positional Fidelity in the Manufacture of a Continuous Fiber Reinforced Thermoplastic Matrix Composite via Pultrusion-Like Processing of Commingled Feedstock

NASA Astrophysics Data System (ADS)

Warlick, Kent M.

While the addition of short fiber to 3D printed articles has increased structural performance, ultimate gains will only be realized through the introduction of continuous reinforcement placed along pre-planned load paths. Most additive manufacturing research focusing on the addition of continuous reinforcement has revolved around utilization of a prefrabricated composite filament or a fiber and matrix mixed within a hot end prior to deposition on a printing surface such that conventional extrusion based FDM can be applied. Although stronger 3D printed parts can be made in this manner, high quality homogenous composites are not possible due to fiber dominated regions, matrix dominated regions, and voids present between adjacent filaments. Conventional composite manufacturing processes are much better at creating homogeneous composites; however, the layer by layer approach in which they are made is inhibiting the alignment of reinforcement with loads. Automated Fiber Placement techniques utilize in plane bending deformation of the tow to facilitate tow steering. Due to buckling fibers on the inner radius of curves, manufacturers recommend a minimum curvature for path placement with this technique. A method called continuous tow shearing has shown promise to enable the placement of tows in complex patterns without tow buckling, spreading, and separation inherent in conventional forms of automated reinforcement positioning. The current work employs fused deposition modeling hardware and the continuous tow shearing technique to manufacture high quality fiber reinforced composites with high positional fidelity, varying continuous reinforcement orientations within a layer, and plastic elements incorporated enabling the ultimate gains in structural performance possible. A mechanical system combining concepts of additive manufacturing with fiber placement via filament winding was developed. Paths with and without tension inherent in filament winding were analyzed through microscopy in order to examine best and worst case scenarios. High quality fiber reinforced composite materials, in terms of low void content, high fiber volume fractions and homogeneity in microstructure, were manufactured in both of these scenarios. In order to improve fidelity and quality in fiber path transition regions, a forced air cooling manifold was designed, printed, and implemented into the current system. To better understand the composite performance that results from varying pertinent manufacturing parameters, the effect of feed rate, hot end temperature, forced air cooling, and deposition surface (polypropylene and previously deposited glass polypropylene commingled tow) on interply performance, microstructure, and positional fidelity were analyzed. Interply performance, in terms of average maximum load and average peel strength, was quantified through a t-peel test of the bonding quality between two surfaces. With use of forced air cooling, minor decreases in average peel strength were present due to a reduction in tow deposition temperature which was found to be the variable most indicative of performance. Average maximum load was comparable between the forced air cooled and non-air cooled samples. Microstructure was evaluated through characterization of composite area, void content, and flash percentage. Low void contents mostly between five to seven percent were attained. Further reduction of this void content to two percent is possible through higher processing temperatures; however, reduced composite area, low average peel strength performance, and the presence of smoke during manufacturing implied thermal degradation of the polypropylene matrix occurred in these samples with higher processing temperatures. Positional fidelity was measured through calculations of shear angle, shift width, and error of a predefined path. While positional fidelity variation was low with a polypropylene deposition surface, forced air cooling is necessary to achieve fidelity on top of an already deposited tow surface as evident by the fifty-six percent reduction in error tolerance profile achieved. Lastly, proof of concept articles with unique fiber paths and neat plastic elements incorporated were produced to demonstrate fiber placement along pre-planned load paths and the ability to achieve greater structural efficiency through the use of less material. The results show that high positional fidelity and high quality composites can be produced through the use of the tow shearing technique implemented in the developed mechanical system. The implementation of forced air cooling was critical in achieving fidelity and quality in transition regions. Alignment of continuous reinforcement with pre-planned load paths was demonstrated in the proof of concept article with varying fiber orientations within a layer. Combining fused deposition modeling of plastic with the placement of continuous reinforcement enabled a honeycomb composite to be produced with higher specific properties than traditional composites. Thus, the current system demonstrated a greater capability of achieving ultimate gains in structural performance than previously possible.

Phase Aberration and Attenuation Effects on Acoustic Radiation Force-Based Shear Wave Generation.

PubMed

Carrascal, Carolina Amador; Aristizabal, Sara; Greenleaf, James F; Urban, Matthew W

2016-02-01

Elasticity is measured by shear wave elasticity imaging (SWEI) methods using acoustic radiation force to create the shear waves. Phase aberration and tissue attenuation can hamper the generation of shear waves for in vivo applications. In this study, the effects of phase aberration and attenuation in ultrasound focusing for creating shear waves were explored. This includes the effects of phase shifts and amplitude attenuation on shear wave characteristics such as shear wave amplitude, shear wave speed, shear wave center frequency, and bandwidth. Two samples of swine belly tissue were used to create phase aberration and attenuation experimentally. To explore the phase aberration and attenuation effects individually, tissue experiments were complemented with ultrasound beam simulations using fast object-oriented C++ ultrasound simulator (FOCUS) and shear wave simulations using finite-element-model (FEM) analysis. The ultrasound frequency used to generate shear waves was varied from 3.0 to 4.5 MHz. Results: The measured acoustic pressure and resulting shear wave amplitude decreased approximately 40%-90% with the introduction of the tissue samples. Acoustic intensity and shear wave displacement were correlated for both tissue samples, and the resulting Pearson's correlation coefficients were 0.99 and 0.97. Analysis of shear wave generation with tissue samples (phase aberration and attenuation case), measured phase screen, (only phase aberration case), and FOCUS/FEM model (only attenuation case) showed that tissue attenuation affected the shear wave generation more than tissue aberration. Decreasing the ultrasound frequency helped maintain a focused beam for creation of shear waves in the presence of both phase aberration and attenuation.

A facile method to align carbon nanotubes on polymeric membrane substrate

PubMed Central

Zhao, Haiyang; Zhou, Zhijun; Dong, Hang; Zhang, Lin; Chen, Huanlin; Hou, Lian

2013-01-01

The alignment of carbon nanotubes (CNT) is the fundamental requirement to ensure their excellent functions but seems to be desolated in recent years. A facile method, hot-press combined with peel-off (HPPO), is introduced here, through which CNT can be successfully vertically aligned on the polymeric membrane substrate. Shear force and mechanical stretch are proposed to be the main forces to align the tubes perpendicular to the substrate surface during the peel-off process. The alignment of CNT keeps its orientation in a thin hybrid membrane by dip-coating cellulose acetate dope solution. It is expected that the stable alignment of CNT by HPPO would contribute to the realization of its potential applications. PMID:24326297

Mechanics of wheel-soil interaction

NASA Technical Reports Server (NTRS)

Houland, H. J.

1973-01-01

An approximate theory for wheel-soil interaction is presented which forms the basis for a practical solution to the problem. It is shown that two fundamental observations render the problem determinate: (1) The line of action of the resultant of radial stresses acting at the wheel soil interface approximately bisects the wheel-soil contact angle for all values of slip. (2) A shear stress surface can be hypothesized. The influence of soil inertia forces is also evaluated. A concept of equivalent cohesion is introduced which allows a convenient experimental comparison for both cohesive and frictional soils. This theory compares favorably with previous analyses and experimental data, and shows that soil inertia forces influencing the motion of a rolling wheel can be significant.

ESTIMATION OF EFFECTIVE SHEAR STRESS WORKING ON FLAT SHEET MEMBRANE USING FLUIDIZED MEDIA IN MBRs

NASA Astrophysics Data System (ADS)

Zaw, Hlwan Moe; Li, Tairi; Nagaoka, Hiroshi; Mishima, Iori

This study was aimed at estimating effective shear stress working on flat sheet membrane by the addition of fluidized media in MBRs. In both of laboratory-scale aeration tanks with and without fluidized media, shear stress variations on membrane surface and water phase velocity variations were measured and MBR operation was conducted. For the evaluation of the effective shear stress working on membrane surface to mitigate membrane surface, simulation of trans-membrane pressure increase was conducted. It was shown that the time-averaged absolute value of shear stress was smaller in the reactor with fluidized media than without fluidized media. However, due to strong turbulence in the reactor with fluidized media caused by interaction between water-phase and media and also due to the direct interaction between membrane surface and fluidized media, standard deviation of shear stress on membrane surface was larger in the reactor with fluidized media than without media. Histograms of shear stress variation data were fitted well to normal distribution curves and mean plus three times of standard deviation was defined to be a maximum shear stress value. By applying the defined maximum shear stress to a membrane fouling model, trans-membrane pressure curve in the MBR experiment was simulated well by the fouling model indicting that the maximum shear stress, not time-averaged shear stress, can be regarded as an effective shear stress to prevent membrane fouling in submerged flat-sheet MBRs.

Sudden Relaminarization and Lifetimes in Forced Isotropic Turbulence.

PubMed

Linkmann, Moritz F; Morozov, Alexander

2015-09-25

We demonstrate an unexpected connection between isotropic turbulence and wall-bounded shear flows. We perform direct numerical simulations of isotropic turbulence forced at large scales at moderate Reynolds numbers and observe sudden transitions from a chaotic dynamics to a spatially simple flow, analogous to the laminar state in wall bounded shear flows. We find that the survival probabilities of turbulence are exponential and the typical lifetimes increase superexponentially with the Reynolds number. Our results suggest that both isotropic turbulence and wall-bounded shear flows qualitatively share the same phase-space dynamics.

Surface phonons and elastic surface waves

NASA Astrophysics Data System (ADS)

Büscher, H.; Klein-Heßling, W.; Ludwig, W.

Theoretical investigations on the dynamics of the (001), (110) and (111) surfaces of some cubic metals (Ag, Cu, Ni) will be reviewed. Both, lattice dynamical and continuum theoretical results are obtained via a Green's function formalism. The main attitude of this paper is the comparison of our results with experiments and with results obtained via slab-calculations. The calculation of elastic surface waves has been performed using a modified surface-green-function-matching method. We have used two different approaches of calculation the bulk Green's function (a) using the spectral representation and (b) a method, what works on residues. The investigations are carried out using shortrange phenomenological potentials. The atomic force constants in the first surface layers are modified to describe surface phonon anomalies, observed by experiments. In the case of Ag (100) and Ag(110) we conclude that the detection of odd symmetry shear modes by Erskine et al. [1 a, b] was not very accurate.

Identification of the functional groups on the surface of nanoparticles formed in photonucleation of aldehydes generated during forest fire events

NASA Astrophysics Data System (ADS)

Dultsev, Fedor N.; Mik, Ivan A.; Dubtsov, Sergei N.; Dultseva, Galina G.

2014-11-01

We describe the new procedure developed to determine the functional groups on the surface of nanoparticles formed in photonucleation of furfural, one of the aldehydes generated during forest fire events. The procedure is based on the detection of nanoparticle rupture from chemically modified surface of the quartz crystal microbalance oscillating in the thickness shear mode under voltage sweep. The rupture force is determined from the voltage at which the rupture occurs. It depends on particle mass and on the affinity of the surface functional groups of the particle to the groups that are present on the modified QCM surface. It was demonstrated with the amine modification of the surface that the nanoparticles formed in furfural photonucleation contain carbonyl and carboxyl groups. The applicability of the method for the investigation of functional groups on the surface of the nanoparticles of atmospheric aerosol is demonstrated.

Shear wave elastography with a new reliability indicator.

PubMed

Dietrich, Christoph F; Dong, Yi

2016-09-01

Non-invasive methods for liver stiffness assessment have been introduced over recent years. Of these, two main methods for estimating liver fibrosis using ultrasound elastography have become established in clinical practice: shear wave elastography and quasi-static or strain elastography. Shear waves are waves with a motion perpendicular (lateral) to the direction of the generating force. Shear waves travel relatively slowly (between 1 and 10 m/s). The stiffness of the liver tissue can be assessed based on shear wave velocity (the stiffness increases with the speed). The European Federation of Societies for Ultrasound in Medicine and Biology has published Guidelines and Recommendations that describe these technologies and provide recommendations for their clinical use. Most of the data available to date has been published using the Fibroscan (Echosens, France), point shear wave speed measurement using an acoustic radiation force impulse (Siemens, Germany) and 2D shear wave elastography using the Aixplorer (SuperSonic Imagine, France). More recently, also other manufacturers have introduced shear wave elastography technology into the market. A comparison of data obtained using different techniques for shear wave propagation and velocity measurement is of key interest for future studies, recommendations and guidelines. Here, we present a recently introduced shear wave elastography technology from Hitachi and discuss its reproducibility and comparability to the already established technologies.

Shear wave elastography with a new reliability indicator

PubMed Central

Dong, Yi

2016-01-01

Non-invasive methods for liver stiffness assessment have been introduced over recent years. Of these, two main methods for estimating liver fibrosis using ultrasound elastography have become established in clinical practice: shear wave elastography and quasi-static or strain elastography. Shear waves are waves with a motion perpendicular (lateral) to the direction of the generating force. Shear waves travel relatively slowly (between 1 and 10 m/s). The stiffness of the liver tissue can be assessed based on shear wave velocity (the stiffness increases with the speed). The European Federation of Societies for Ultrasound in Medicine and Biology has published Guidelines and Recommendations that describe these technologies and provide recommendations for their clinical use. Most of the data available to date has been published using the Fibroscan (Echosens, France), point shear wave speed measurement using an acoustic radiation force impulse (Siemens, Germany) and 2D shear wave elastography using the Aixplorer (SuperSonic Imagine, France). More recently, also other manufacturers have introduced shear wave elastography technology into the market. A comparison of data obtained using different techniques for shear wave propagation and velocity measurement is of key interest for future studies, recommendations and guidelines. Here, we present a recently introduced shear wave elastography technology from Hitachi and discuss its reproducibility and comparability to the already established technologies. PMID:27679731

Graphene Coating via Chemical Vapor Deposition for Improving Friction and Wear of Gray Cast Iron at Interfaces.

PubMed

Tripathi, Khagendra; Gyawali, Gobinda; Lee, Soo Wohn

2017-09-20

This study reports the influence of CVD-graphene on the tribological performance of gray cast iron (GCI) from the internal combustion engine (ICE) cylinder liners by performing a ball-on-disk friction tests. The graphene-coated specimen exhibited a significant reduction (∼53%) of friction as compared to that of the uncoated specimen, whereas wear resistance increased by 2- and 5-fold regarding the wear of specimen and ball, respectively. Extremely low shear strength and highly lubricating nature of graphene contribute to the formation of a lubricative film between the sliding surfaces and decreases the interaction between surfaces in the dry environment. Under the applied load, a uniform film of iron oxides such as Fe 2 O 3 , Fe 3 O 4 , and FeOOH is found to be formed between the surfaces. It is proposed that the graphene encapsulation with the metal debris and oxides formed between the specimens increases the lubricity and decreases the shear force. The transformation of graphene/graphite into nanocrystalline graphites across the contact interfaces following the amorphization trajectory further increases the lubricity of the film that ultimately reduces friction and wear of the material.

Modeling cell-substrate de-adhesion dynamics under fluid shear

NASA Astrophysics Data System (ADS)

Maan, Renu; Rani, Garima; Menon, Gautam I.; Pullarkat, Pramod A.

2018-07-01

Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. Here, we investigate, theoretically and experimentally, the detachment of cells adhered to substrates when these cells are subjected to fluid shear. We present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as the decay in this fraction at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, we extract characteristic force scales for cell adhesion as well as characteristic detachment times. We estimate force-scales of  ∼500 pN associated to a single focal contact, and characteristic time-scales of s representing cell-spread-area dependent mean first passage times to the detached state at intermediate values of the shear stress. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis.

Propagation of shock waves in elastic solids caused by cavitation microjet impact. II: Application in extracorporeal shock wave lithotripsy.

PubMed

Zhong, P; Chuong, C J; Preminger, G M

1993-07-01

To better understand the mechanism of stone fragmentation during extracorporeal shock wave lithotripsy (ESWL), the model developed in Part I [P. Zhong and C.J. Chuong, J. Acoust. Soc. Am. 94, 19-28 (1993)] is applied to study cavitation microjet impingement and its resultant shock wave propagation in renal calculi. Impact pressure at the stone boundary and stress, strain at the propagating shock fronts in the stone were calculated for typical ESWL loading conditions. At the anterior surface of the stone, the jet induced compressive stress can vary from 0.82 approximately 4 times that of the water hammer pressure depending on the contact angles; whereas the jet-induced shear stress can achieve its maximum, with a magnitude of 30% approximately 54% of the water hammer pressure, near the detachment of the longitudinal (or P) wave in the solid. Comparison of model predictions with material failure strengths of renal calculi suggests that jet impact can lead to stone surface erosion by combined compressive and shear loadings at the jet impacting surface, and spalling failure by tensile forces at the distal surface of the stone. Comparing responses from four different stone types suggests that cystine is the most difficult stone to fragment in ESWL, as observed from clinical experience.

Modeling Force Transfer around Openings in Wood-Frame Shear Walls

Treesearch

Minghao Li; Frank Lam; Borjen Yeh; Tom Skaggs; Doug Rammer; James Wacker

2012-01-01

This paper presented a modeling study on force transfer around openings (FTAO) in wood-frame shear walls detailed for FTAO. To understand the load transfer in the walls, this study used a finite-element model WALL2D, which is able to model individual wall components, including framing members, sheathing panels, oriented panel-frame nailed connections, framing...

Pressure effects on the nose by an in-flight oxygen mask during simulated flight conditions.

PubMed

Schreinemakers, J Rieneke C; Boer, C; van Amerongen, P C G M; Kon, M

2016-12-01

Dutch F-16 fighter pilots experience oxygen mask inflicted nasal trauma, including discomfort, pain, skin abrasions, bruises and bone remodelling. Pressure and shear forces on the nose might contribute to causing these adverse effects. In this study, it was evaluated how flight conditions affected the exerted pressure, and whether shear forces were present. The pressure exerted by the oxygen mask was measured in 20 volunteers by placing pressure sensors on the nose and chin underneath the mask. In the human centrifuge, the effects on the exerted pressure during different flight conditions were evaluated (+3G z , +6G z , +9G z , protocolised head movements, mounted visor or night vision goggles, NVG). The runs were recorded to evaluate if the mask's position changed during the run, which would confirm the presence of shear forces. Head movements increased the median pressure on the nose by 50 mm Hg and on the chin by 37 mm Hg. NVG, a visor and accelerative forces also increased the median pressure on the nose. Pressure drops on the nose were also observed, during mounted NVG (-63 mm Hg). The recordings showed the mask slid downwards, especially during the acceleration phase of the centrifuge run, signifying the presence of shear forces. The exerted pressure by the oxygen mask changes during different flight conditions. Exposure to changing pressures and to shear forces probably contributes to mask-inflicted nasal trauma. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

Platelet-free shear flow assay facilitates analysis of shear-dependent functions of VWF and ADAMTS13.

PubMed

Kraus, Emma; Kraus, Kristina; Obser, Tobias; Oyen, Florian; Klemm, Ulrike; Schneppenheim, Reinhard; Brehm, Maria A

2014-12-01

The multimeric form of von Willebrand factor (VWF), is the largest soluble protein in mammals and exhibits a multidomain structure resulting in multiple functions. Upon agonist stimulation endothelial cells secrete VWF multimers from Weibel-Palade bodies into the blood stream where VWF plays an essential role in platelet-dependent primary hemostasis. Elongation of VWF strings on the cells' surface leads to accessibility of VWF binding sites for proteins, such as platelet membrane glycoprotein Ib. The prothrombotic strings are size-regulated by the metalloprotease ADAMTS13 by shear force-activated proteolytic cleavage. VWF string formation was induced by histamine stimulation of HUVEC cells under unidirectional shear flow and VWF strings were detected employing the VWF binding peptide of platelet glycoprotein Ib coupled to latex beads. VWF strings were then used as substrate for kinetic studies of recombinant and plasma ADAMTS13. To investigate specific aspects of the shear-dependent functions of VWF and ADAMTS13, we developed a shear flow assay that allows observation of VWF string formation and their degradation by ADAMTS13 without the need for isolated platelets. Our assay specifically detects VWF strings, can be coupled with fluorescent applications and allows semi-automated, quantitative assessment of recombinant and plasma ADAMTS13 activity. Our assay may serve as a valuable research tool to investigate the biochemical characteristics of VWF and ADAMTS13 under shear flow and could complement diagnostics of von Willebrand Disease and Thrombotic Thrombocytopenic Purpura as it allows detection of shear flow-dependent dysfunction of VWD-associated VWF mutants as well as TTP-associated ADAMTS13 mutants. Copyright © 2014 Elsevier Ltd. All rights reserved.

Nano- to microscale dynamics of P-selectin detachment from leukocyte interfaces. II. Tether flow terminated by P-selectin dissociation from PSGL-1.

PubMed

Heinrich, Volkmar; Leung, Andrew; Evans, Evan

2005-03-01

We have used a biomembrane force probe decorated with P-selectin to form point attachments with PSGL-1 receptors on a human neutrophil (PMN) in a calcium-containing medium and then to quantify the forces experienced by the attachment during retraction of the PMN at fixed speed. From first touch to final detachment, the typical force history exhibited the following sequence of events: i), an initial linear-elastic displacement of the PMN surface, ii), an abrupt crossover to viscoplastic flow that signaled membrane separation from the interior cytoskeleton and the beginning of a membrane tether, and iii), the final detachment from the probe tip most often by one precipitous step of P-selectin:PSGL-1 dissociation. Analyzing the initial elastic response and membrane unbinding from the cytoskeleton in our companion article I, we focus in this article on the regime of tether extrusion that nearly always occurred before release of the extracellular adhesion bond at pulling speeds > or =1 microm/s. The force during tether growth appeared to approach a plateau at long times. Examined over a large range of pulling speeds up to 150 microm/s, the plateau force exhibited a significant shear thinning as indicated by a weak power-law dependence on pulling speed, f(infinity) = 60 pN(nu(pull)/microm/s)(0.25). Using this shear-thinning response to describe the viscous element in a nonlinear Maxwell-like fluid model, we show that a weak serial-elastic component with a stiffness of approximately 0.07 pN/nm provides good agreement with the time course of the tether force approach to the plateau under constant pulling speed.

Calculation and analysis of shear resistance of segment ring joint with shear pin

NASA Astrophysics Data System (ADS)

Wu, Shengzhi; Huang, Haibin; Wang, Mingnian; Xiao, Shihui; Liu, Dagang

2018-03-01

In order to get the effect of shear pins between segments on the shear resistance of segment girth joints. Take the Maliuzhou traffic tunnel project of Zhuhai which with super large diameter and Marine Composite strata as the research object, the longitudinal shear stiffness of tunnel shear considering the shear rigidity of shear pins was obtained through the finite element shear experiment of segment ring. By comparing the calculation results of shear pin and non shear pin between segment ring connections, the conclusion that shear pin setting can effectively decompose and transfer shear force and control the dislocation between segment ring blocks is obtained. The study can be used as reference for the design and construction of shield tunnel.

Influence of complex interfacial rheology on the thermocapillary migration of a surfactant-laden droplet in Poiseuille flow

NASA Astrophysics Data System (ADS)

Das, Sayan; Chakraborty, Suman

2018-02-01

The effect of surface viscosity on the motion of a surfactant-laden droplet in the presence of a non-isothermal Poiseuille flow is studied, both analytically and numerically. The presence of bulk-insoluble surfactants along the droplet surface results in interfacial shear and dilatational viscosities. This, in turn, is responsible for the generation of surface-excess viscous stresses that obey the Boussinesq-Scriven constitutive law for constant values of surface shear and dilatational viscosities. The present study is primarily focused on finding out how this confluence can be used to modulate droplet dynamics in the presence of Marangoni stress induced by nonuniform distribution of surfactants and temperature along the droplet surface, by exploiting an intricate interplay of the respective forcing parameters influencing the interfacial stresses. Under the assumption of negligible fluid inertia and thermal convection, the steady-state migration velocity of a non-deformable spherical droplet, placed at the centerline of an imposed unbounded Poiseuille flow, is obtained for the limiting case when the surfactant transport along the interface is dominated by surface diffusion. Our analysis proves that the droplet migration velocity is unaffected by the shear viscosity whereas the dilatational viscosity has a significant effect on the same. The surface viscous effects always retard the migration of a surfactant-laden droplet when the temperature in the far-field increases in the direction of the imposed flow although the droplet always migrates towards the hotter region. On the contrary, if a large temperature gradient is applied in a direction opposite to that of the imposed flow, the direction of droplet migration gets reversed. However, for a sufficiently high value of dilatational surface viscosity, the direction of droplet migration reverses. For the limiting case in which the surfactant transport along the droplet surface is dominated by surface convection, on the other hand, surface viscosities do not have any effect on the motion of the droplet. These results are likely to have far-reaching consequences in designing an optimal migration path in droplet-based microfluidic technology.

Surface quality of silicon wafer improved by hydrodynamic effect polishing

NASA Astrophysics Data System (ADS)

Peng, Wenqiang; Guan, Chaoliang; Li, Shengyi

2014-08-01

Differing from the traditional pad polishing, hydrodynamic effect polishing (HEP) is non-contact polishing with the wheel floated on the workpiece. A hydrodynamic lubricated film is established between the wheel and the workpiece when the wheel rotates at a certain speed in HEP. Nanoparticles mixed with deionized water are employed as the polishing slurry, and with action of the dynamic pressure, nanoparticles with high chemisorption due to the high specific surface area can easily reacted with the surface atoms forming a linkage with workpiece surface. The surface atoms are dragged away when nanoparticles are transported to separate by the flow shear stress. The development of grand scale integration put extremely high requirements on the surface quality on the silicon wafer with surface roughness at subnanometer and extremely low surface damage. In our experiment a silicon sample was processed by HEP, and the surface topography before and after polishing was observed by the atomic force microscopy. Experiment results show that plastic pits and bumpy structures on the initial surface have been removed away clearly with the removal depth of 140nm by HEP process. The processed surface roughness has been improved from 0.737nm RMS to 0.175nm RMS(10μm×10μm) and the section profile shows peaks of the process surface are almost at the same height. However, the machining ripples on the wheel surface will duplicate on the silicon surface under the action of the hydrodynamic effect. Fluid dynamic simulation demonstrated that the coarse surface on the wheel has greatly influence on the distribution of shear stress and dynamic pressure on the workpiece surface.

QCM operating in threshold mode as a gas sensor.

PubMed

Dultsev, Fedor N; Kolosovsky, Eugeny A

2009-10-20

Application of the threshold mode allowed us to use the quartz resonator (quartz crystal microbalance, QCM) as a highly sensitive gas sensor measuring the forces of the rupture of adsorbed gas components from the resonator surface oscillating with increasing amplitude. This procedure allows one to analyze different gas components using the same surface modification, just varying the rupture threshold by varying the amplitude of shear oscillations. The sensitivity of the threshold measurements is 2 to 3 orders of magnitude higher than for the gravimetric procedure. It is demonstrated that the QCM operating as an active element can be used as a gas sensor. This procedure seems to be promising in investigating the reactivity of the surface or the interactions of gaseous components with the surface containing various functional groups, thus contributing to the surface chemistry.

Evaluation of the shear force of single cancer cells by vertically aligned carbon nanotubes suitable for metastasis diagnosis.

PubMed

Abdolahad, M; Mohajerzadeh, S; Janmaleki, M; Taghinejad, H; Taghinejad, M

2013-03-01

Vertically aligned carbon nanotube (VACNT) arrays have been demonstrated as probes for rapid quantifying of cancer cell deformability with high resolution. Through entrapment of various cancer cells on CNT arrays, the deflections of the nanotubes during cell deformation were used to derive the lateral cell shear force using a large deflection mode method. It is observed that VACNT beams act as sensitive and flexible agents, which transfer the shear force of cells trapped on them by an observable deflection. The metastatic cancer cells have significant deformable structures leading to a further cell traction force (CTF) than primary cancerous one on CNT arrays. The elasticity of different cells could be compared by their CTF measurement on CNT arrays. This study presents a nanotube-based methodology for quantifying the single cell mechanical behavior, which could be useful for understanding the metastatic behavior of cells.

Single-cell adhesion probed in-situ using optical tweezers: A case study with Saccharomyces cerevisiae

NASA Astrophysics Data System (ADS)

Castelain, Mickaël; Rouxhet, Paul G.; Pignon, Frédéric; Magnin, Albert; Piau, Jean-Michel

2012-06-01

A facile method of using optical trapping to measure cell adhesion forces is presented and applied to the adhesion of Saccharomyces cerevisiae on glass, in contact with solutions of different compositions. Trapping yeast cells with optical tweezers (OT) is not perturbed by cell wall deformation or cell deviation from a spherical shape. The trapping force calibration requires correction not only for the hydrodynamic effect of the neighboring wall but also for spherical aberrations affecting the focal volume and the trap stiffness. Yeast cells trapped for up to 5 h were still able to undergo budding but showed an increase of doubling time. The proportion of adhering cells showed the expected variation according to the solution composition. The detachment force varied in the same way. This observation and the fact that the detachment stress was exerted parallel to the substrate surface point to the role of interactions involving solvated macromolecules. Both the proportion of adhering cells and the removal force showed a distribution which, in our experimental conditions, must be attributed to a heterogeneity of surface properties at the cell level or at the subcellular scale. As compared with magnetic tweezers, atomic force microscopy, and more conventional ways of studying cell adhesion (shear-flow cells), OT present several advantages that are emphasized in this paper.

Surface waves in an incompressible fluid - Resonant instability due to velocity shear

NASA Technical Reports Server (NTRS)

Hollweg, Joseph V.; Yang, G.; Cadez, V. M.; Gakovic, B.

1990-01-01

The effects of velocity shear on the resonance absorption of incompressible MHD surface waves are studied. It is found that there are generally values of the velocity shear for which the surface wave decay rate becomes zero. In some cases, the resonance absorption goes to zero even for very small velocity shears. It is also found that the resonance absorption can be strongly enhanced at other values of the velocity shear, so the presence of flows may be generally important for determining the effects of resonance absorption, such as might occur in the interaction of p-modes with sunspots. Resonances leading to instability of the global surface mode can exist, and instability can occur for velocity shears significantly below the Kelvin-Helmholtz threshold. These instabilities may play a role in the development or turbulence in regions of strong velocity shear in the solar wind or the earth's magnetosphere.

Limited use of Centritech Lab II Centrifuge in perfusion culture of rCHO cells for the production of recombinant antibody.

PubMed

Kim, Byoung Jin; Oh, Duk Jae; Chang, Ho Nam

2008-01-01

Perfusion cultures of recombinant Chinese hamster ovary cells, producing recombinant antibody against the S surface antigen of Hepatitis B virus, were carried out in continuous and intermittent mode using a Centritech Lab II Centrifuge. In the continuous perfusion process, despite the absence of shear stress from the pump head, long-term operation was not possible because of continuously repeated exposure to oxygen limitation and low temperature, as well as shear stress from centrifugal force. In the intermittent perfusion processes, the frequency of cell-passage through the centrifuge was substantially reduced, compared with the continuous perfusion mode; however, the degree of reduction could not guarantee stable long-term operation. Although various operating parameters were applied in the intermittent perfusion cultures, high cell densities could not be maintained stably. In a single bioreactor culture system, a specific cell that is returned from the centrifuge to the bioreactor could be transferred from the bioreactor to the centrifuge again in the next cycle. These repetitive damages, caused by shear stress from the pump head and centrifugal force, as well as exposure to suboptimal conditions such as oxygen limitation and low temperature below 37 degrees C, were more serious at higher perfusion rates. Subsequently, damaged cells and dead cells were continuously accumulated in the bioreactor. Culture temperature shift from 37 to 33 degrees C increased antibody concentrations but showed inhibitory effects on cell growth. The negative effects of lowering culture temperature on cell growth overwhelmed the positive effects on antibody production. To protect cells from shear stress, Pluronic F-68 was 2-fold concentrated in the culture medium; nevertheless, a significantly higher concentration of Pluronic F-68 (2 g/L) may have inhibitory effects on cell growth.

Near-field non-radial motion generation from underground chemical explosions in jointed granite

NASA Astrophysics Data System (ADS)

Vorobiev, Oleg; Ezzedine, Souheil; Hurley, Ryan

2018-01-01

This paper describes analysis of non-radial ground motion generated by chemical explosions in a jointed rock formation during the Source Physics Experiment (SPE). Such motion makes it difficult to discriminate between various subsurface events such as explosions, implosions (i.e. mine collapse) and earthquakes. We apply 3-D numerical simulations to understand experimental data collected during the SPEs. The joints are modelled explicitly as compliant thin inclusions embedded into the rock mass. Mechanical properties of the rock and the joints as well as the joint spacing and orientation are inferred from experimental test data, and geophysical and geological characterization of the SPE site which is dominantly Climax Stock granitic outcrop. The role of various factors characterizing the joints such as joint spacing, frictional properties, orientation and persistence in generation of non-radial motion is addressed. The joints in granite at the SPE site are oriented in nearly orthogonal directions with two vertical sets dipping at 70-80 degrees with the same strike angle, one vertical set almost orthogonal to the first two and one shallow angle joint set dipping 15 degrees. In this study we establish the relationship between the joint orientation and azimuthal variations in the polarity of the observed shear motion. The majority of the shear motion is generated due to the effects of non-elastic sliding on the joints near the source, where the wave can create significant shear stress to overcome the cohesive forces at the joints. Near the surface the joints are less confined and are subject to sliding when the pressure waves are reflected. In the far field, where the cohesive forces on the joints cannot be overcome, additional shear motion can be generated due to elastic anisotropy of the rock mass given by preferred spatial orientations of compliant joints.

Biomechanics of hair cell kinocilia: experimental measurement of kinocilium shaft stiffness and base rotational stiffness with Euler–Bernoulli and Timoshenko beam analysis

PubMed Central

Spoon, Corrie; Grant, Wally

2011-01-01

Vestibular hair cell bundles in the inner ear contain a single kinocilium composed of a 9+2 microtubule structure. Kinocilia play a crucial role in transmitting movement of the overlying mass, otoconial membrane or cupula to the mechanotransducing portion of the hair cell bundle. Little is known regarding the mechanical deformation properties of the kinocilium. Using a force-deflection technique, we measured two important mechanical properties of kinocilia in the utricle of a turtle, Trachemys (Pseudemys) scripta elegans. First, we measured the stiffness of kinocilia with different heights. These kinocilia were assumed to be homogenous cylindrical rods and were modeled as both isotropic Euler–Bernoulli beams and transversely isotropic Timoshenko beams. Two mechanical properties of the kinocilia were derived from the beam analysis: flexural rigidity (EI) and shear rigidity (kGA). The Timoshenko model produced a better fit to the experimental data, predicting EI=10,400 pN μm2 and kGA=247 pN. Assuming a homogenous rod, the shear modulus (G=1.9 kPa) was four orders of magnitude less than Young's modulus (E=14.1 MPa), indicating that significant shear deformation occurs within deflected kinocilia. When analyzed as an Euler–Bernoulli beam, which neglects translational shear, EI increased linearly with kinocilium height, giving underestimates of EI for shorter kinocilia. Second, we measured the rotational stiffness of the kinocilium insertion (κ) into the hair cell's apical surface. Following BAPTA treatment to break the kinocilial links, the kinocilia remained upright, and κ was measured as 177±47 pN μm rad–1. The mechanical parameters we quantified are important for understanding how forces arising from head movement are transduced and encoded by hair cells. PMID:21307074

Estimation of lumbar spinal loading and trunk muscle forces during asymmetric lifting tasks: application of whole-body musculoskeletal modelling in OpenSim.

PubMed

Kim, Hyun-Kyung; Zhang, Yanxin

2017-04-01

Large spinal compressive force combined with axial torsional shear force during asymmetric lifting tasks is highly associated with lower back injury (LBI). The aim of this study was to estimate lumbar spinal loading and muscle forces during symmetric lifting (SL) and asymmetric lifting (AL) tasks using a whole-body musculoskeletal modelling approach. Thirteen healthy males lifted loads of 7 and 12 kg under two lifting conditions (SL and AL). Kinematic data and ground reaction force data were collected and then processed by a whole-body musculoskeletal model. The results show AL produced a significantly higher peak lateral shear force as well as greater peak force of psoas major, quadratus lumborum, multifidus, iliocostalis lumborum pars lumborum, longissimus thoracis pars lumborum and external oblique than SL. The greater lateral shear forces combined with higher muscle force and asymmetrical muscle contractions may have the biomechanical mechanism responsible for the increased risk of LBI during AL. Practitioner Summary: Estimating lumbar spinal loading and muscle forces during free-dynamic asymmetric lifting tasks with a whole-body musculoskeletal modelling in OpenSim is the core value of this research. The results show that certain muscle groups are fundamentally responsible for asymmetric movement, thereby producing high lumbar spinal loading and muscle forces, which may increase risks of LBI during asymmetric lifting tasks.

Rheology of the lithosphere: selected topics.

USGS Publications Warehouse

Kirby, S.H.; Kronenberg, A.K.

1987-01-01

Reviews recent results concerning the rheology of the lithosphere with special attention to the following topics: 1) the flexure of the oceanic lithosphere, 2) deformation of the continental lithosphere resulting from vertical surface loads and forces applied at plate margins, 3) the rheological stratification of the continents, 4) strain localization and shear zone development, and 5) strain-induced crystallographic preferred orientations and anisotropies in body-wave velocities. We conclude with a section citing the 1983-1986 rock mechanics literature by category.-Authors

Random three-dimensional jammed packings of elastic shells acting as force sensors

NASA Astrophysics Data System (ADS)

Jose, Jissy; van Blaaderen, Alfons; Imhof, Arnout

2016-06-01

In a jammed solid of granular particles, the applied stress is in-homogeneously distributed within the packing. A full experimental characterization requires measurement of all the interparticle forces, but so far such measurements are limited to a few systems in two and even fewer in three dimensions. Particles with the topology of (elastic) shells are good local force sensors as relatively large deformations of the shells result from relatively small forces. We recently introduced such fluorescent shells as a model granular system in which force distributions can be determined in three dimensions using confocal microscopy and quantitative image analysis. An interesting aspect about these shells that differentiates them from other soft deformable particles is their buckling behavior at higher compression. This leads to deformations that do not conserve the inner volume of the particle. Here we use this system to accurately measure the contact forces in a three-dimensional packing of shells subjected to a static anisotropic compression and to shear. At small deformations forces are linear, however, for a buckled contact, the restoring force is related to the amount of deformation by a square root law, as follows from the theory of elasticity of shells. Near the unjamming-jamming transition (point J ), we found the probability distribution of the interparticle forces P (f ) to decay nearly exponentially at large forces, with little evidence of long-range force chains in the packings. As the packing density is increased, the tail of the distribution was found to crossover to a Gaussian, in line with other experimental and simulation studies. Under a small shear strain, up to 0.216, applied at an extremely low shear rate, we observed a shear-induced anisotropy in both the pair correlation function and contact force network; however, no appreciable change was seen in the number of contacts per particle.

Micro-mechanics of electrostatically stabilized suspensions of cellulose nanofibrils under steady state shear flow.

PubMed

Martoïa, F; Dumont, P J J; Orgéas, L; Belgacem, M N; Putaux, J-L

2016-02-14

In this study, we characterized and modeled the rheology of TEMPO-oxidized cellulose nanofibril (NFC) aqueous suspensions with electrostatically stabilized and unflocculated nanofibrous structures. These colloidal suspensions of slender and wavy nanofibers exhibited a yield stress and a shear thinning behavior at low and high shear rates, respectively. Both the shear yield stress and the consistency of these suspensions were power-law functions of the NFC volume fraction. We developed an original multiscale model for the prediction of the rheology of these suspensions. At the nanoscale, the suspensions were described as concentrated systems where NFCs interacted with the Newtonian suspending fluid through Brownian motion and long range fluid-NFC hydrodynamic interactions, as well as with each other through short range hydrodynamic and repulsive colloidal interaction forces. These forces were estimated using both the experimental results and 3D networks of NFCs that were numerically generated to mimic the nanostructures of NFC suspensions under shear flow. They were in good agreement with theoretical and measured forces for model colloidal systems. The model showed the primary role played by short range hydrodynamic and colloidal interactions on the rheology of NFC suspensions. At low shear rates, the origin of the yield stress of NFC suspensions was attributed to the combined contribution of repulsive colloidal interactions and the topology of the entangled NFC networks in the suspensions. At high shear rates, both concurrent colloidal and short (in some cases long) range hydrodynamic interactions could be at the origin of the shear thinning behavior of NFC suspensions.

Numerical investigations of two-degree-of-freedom vortex-induced vibration in shear flow

NASA Astrophysics Data System (ADS)

Zhang, Hui; Liu, Mengke; Han, Yang; Li, Jian; Gui, Mingyue; Chen, Zhihua

2017-06-01

Exponential-polar coordinates attached to a moving cylinder are used to deduce the stream function-vorticity equations for two-degree-of-freedom vortex-induced vibration, the initial and boundary conditions, and the distribution of the hydrodynamic force, which consists of the vortex-induced force, inertial force, and viscous damping force. The fluid-structure interactions occurring from the motionless cylinder to the steady vibration are investigated numerically, and the variations of the flow field, pressure, lift/drag, and cylinder displacement are discussed. Both the dominant vortex and the cylinder shift, whose effects are opposite, affect the shear layer along the transverse direction and the secondary vortex along the streamwise direction. However, the effect of the cylinder shift is larger than that of the dominant vortices. Therefore, the former dominates the total effects of the flow field. Moreover, the symmetry of the flow field is broken with the increasing shear rate. With the effect of the background vortex, the upper vortices are strengthened, and the lower vortices are weakened; thus, the shear layer and the secondary vortices induced by the upper shedding vortices are strengthened, while the shear layer and the secondary vortices induced by the lower shedding vortices are weakened. Therefore, the amplitudes of the displacement and drag/lift dominated by the upper vortex are larger than those of the displacement and drag/lift dominated by the lower vortex.

Mechanical forces regulate the reactivity of a thioester bond in a bacterial adhesin

PubMed Central

Echelman, Daniel J.; Lee, Alex Q.; Fernández, Julio M.

2017-01-01

Bacteria must withstand large mechanical shear forces when adhering to and colonizing hosts. Recent structural studies on a class of Gram-positive bacterial adhesins have revealed an intramolecular Cys-Gln thioester bond that can react with surface-associated ligands to covalently anchor to host surfaces. Two other examples of such internal thioester bonds occur in certain anti-proteases and in the immune complement system, both of which react with the ligand only after the thioester bond is exposed by a proteolytic cleavage. We hypothesized that mechanical forces in bacterial adhesion could regulate thioester reactivity to ligand analogously to such proteolytic gating. Studying the pilus tip adhesin Spy0125 of Streptococcus pyogenes, we developed a single molecule assay to unambiguously resolve the state of the thioester bond. We found that when Spy0125 was in a folded state, its thioester bond could be cleaved with the small-molecule nucleophiles methylamine and histamine, but when Spy0125 was mechanically unfolded and subjected to forces of 50–350 piconewtons, thioester cleavage was no longer observed. For folded Spy0125 without mechanical force exposure, thioester cleavage was in equilibrium with spontaneous thioester reformation, which occurred with a half-life of several minutes. Functionally, this equilibrium reactivity allows thioester-containing adhesins to sample potential substrates without irreversible cleavage and inactivation. We propose that such reversible thioester reactivity would circumvent potential soluble inhibitors, such as histamine released at sites of inflammation, and allow the bacterial adhesin to selectively associate with surface-bound ligands. PMID:28348083

A scheme for computing surface layer turbulent fluxes from mean flow surface observations

NASA Technical Reports Server (NTRS)

Hoffert, M. I.; Storch, J.

1978-01-01

A physical model and computational scheme are developed for generating turbulent surface stress, sensible heat flux and humidity flux from mean velocity, temperature and humidity at some fixed height in the atmospheric surface layer, where conditions at this reference level are presumed known from observations or the evolving state of a numerical atmospheric circulation model. The method is based on coupling the Monin-Obukov surface layer similarity profiles which include buoyant stability effects on mean velocity, temperature and humidity to a force-restore formulation for the evolution of surface soil temperature to yield the local values of shear stress, heat flux and surface temperature. A self-contained formulation is presented including parameterizations for solar and infrared radiant fluxes at the surface. Additional parameters needed to implement the scheme are the thermal heat capacity of the soil per unit surface area, surface aerodynamic roughness, latitude, solar declination, surface albedo, surface emissivity and atmospheric transmissivity to solar radiation.

Cysteine shotgun–mass spectrometry (CS-MS) reveals dynamic sequence of protein structure changes within mutant and stressed cells

PubMed Central

Krieger, Christine C.; An, Xiuli; Tang, Hsin-Yao; Mohandas, Narla; Speicher, David W.; Discher, Dennis E.

2011-01-01

Questions of if and when protein structures change within cells pervade biology and include questions of how the cytoskeleton sustains stresses on cells—particularly in mutant versus normal cells. Cysteine shotgun labeling with fluorophores is analyzed here with mass spectrometry of the spectrin–actin membrane skeleton in sheared red blood cell ghosts from normal and diseased mice. Sheared samples are compared to static samples at 37 °C in terms of cell membrane intensity in fluorescence microscopy, separated protein fluorescence, and tryptic peptide modification in liquid chromatography–tandem mass spectrometry (LC-MS/MS). Spectrin labeling proves to be the most sensitive to shear, whereas binding partners ankyrin and actin exhibit shear thresholds in labeling and both the ankyrin-binding membrane protein band 3 and the spectrin–actin stabilizer 4.1R show minimal differential labeling. Cells from 4.1R-null mice differ significantly from normal in the shear-dependent labeling of spectrin, ankyrin, and band 3: Decreased labeling of spectrin reveals less stress on the mutant network as spectrin dissociates from actin. Mapping the stress-dependent labeling kinetics of α- and β-spectrin by LC-MS/MS identifies Cys in these antiparallel chains that are either force-enhanced or force-independent in labeling, with structural analyses indicating the force-enhanced sites are sequestered either in spectrin’s triple-helical domains or in interactions with actin or ankyrin. Shear-sensitive sites identified comprehensively here in both spectrin and ankyrin appear consistent with stress relief through forced unfolding followed by cytoskeletal disruption. PMID:21527722

Simulating Fiber Ordering and Aggregation In Shear Flow Using Dissipative Particle Dynamics

NASA Astrophysics Data System (ADS)

Stimatze, Justin T.

We have developed a mesoscale simulation of fiber aggregation in shear flow using LAMMPS and its implementation of dissipative particle dynamics. Understanding fiber aggregation in shear flow and flow-induced microstructural fiber networks is critical to our interest in high-performance composite materials. Dissipative particle dynamics enables the consideration of hydrodynamic interactions between fibers through the coarse-grained simulation of the matrix fluid. Correctly simulating hydrodynamic interactions and accounting for fluid forces on the microstructure is required to correctly model the shear-induced aggregation process. We are able to determine stresses, viscosity, and fiber forces while simulating the evolution of a model fiber system undergoing shear flow. Fiber-fiber contact interactions are approximated by combinations of common pairwise forces, allowing the exploration of interaction-influenced fiber behaviors such as aggregation and bundling. We are then able to quantify aggregate structure and effective volume fraction for a range of relevant system and fiber-fiber interaction parameters. Our simulations have demonstrated several aggregate types dependent on system parameters such as shear rate, short-range attractive forces, and a resistance to relative rotation while in contact. A resistance to relative rotation at fiber-fiber contact points has been found to strongly contribute to an increased angle between neighboring aggregated fibers and therefore an increase in average aggregate volume fraction. This increase in aggregate volume fraction is strongly correlated with a significant enhancement of system viscosity, leading us to hypothesize that controlling the resistance to relative rotation during manufacturing processes is important when optimizing for desired composite material characteristics.

Biorobotic adhesion in water using suction cups.

PubMed

Bandyopadhyay, Promode R; Hrubes, J Dana; Leinhos, Henry A

2008-03-01

Echeneid fish, limpets and octopi use suction cups for underwater adhesion. When echeneid fish use suckers to 'hitch a ride' on sharks (which have riblet-patterned skins), the apparent absence of any pump or plumbing may be an advantage over biorobotic suction cups. An intriguing question is: How do they achieve seemingly persistent leak-free contact at low energy cost over rough surfaces? The design features of their suckers are explored in a biorobotic context of adhesion in water over rough surfaces. We have carried out experiments to compare the release force and tenacity of man-made suction cups with those reported for limpets and echeneid fish. Applied tensile and shear release forces were monotonically increased until release. The effects of cup size and type, host surface roughness, curvature and liquid surface tension have been examined. The flow of water in the sharkskin-like host surface roughness has been characterized. The average tenacity is 5.28 N cm(-2) (sigma = 0.53 N cm(-2), N = 37) in the sub-ambient pressure range of 14.6-49.0 kPa, in man-made cups for monotonically increasing applied release force. The tenacity is lower for harmonically oscillating release forces. The dynamic structural interactions between the suction cup and the oscillating applied forcing are discussed. Inspired by the matching of sharkskin riblet topology in echeneid fish suckers, it was found that biorobotic sealed contact over rough surfaces is also feasible when the suction cup makes a negative copy of the rough host surface. However, for protracted, persistent contact, the negative topology would have to be maintained by active means. Energy has to be spent to maintain the negative host roughness topology to minute detail, and protracted hitch-riding on sharks for feeding may not be free for echeneid fish. Further work is needed on the mechanism and efficiency of the densely populated tiny actuators in the fish suckers that maintain leak-proof contact with minimal energy cost and the feasibility of their biorobotic replication.

Shear Behaviour and Acoustic Emission Characteristics of Bolted Rock Joints with Different Roughnesses

NASA Astrophysics Data System (ADS)

Wang, Gang; Zhang, Yongzheng; Jiang, Yujing; Liu, Peixun; Guo, Yanshuang; Liu, Jiankang; Ma, Ming; Wang, Ke; Wang, Shugang

2018-06-01

To study shear failure, acoustic emission counts and characteristics of bolted jointed rock-like specimens are evaluated under compressive shear loading. Model joint surfaces with different roughnesses are made of rock-like material (i.e. cement). The jointed rock masses are anchored with bolts with different elongation rates. The characteristics of the shear mechanical properties, the failure mechanism, and the acoustic emission parameters of the anchored joints are studied under different surface roughnesses and anchorage conditions. The shear strength and residual strength increase with the roughness of the anchored joint surface. With an increase in bolt elongation, the shear strength of the anchored joint surface gradually decreases. When the anchored structural plane is sheared, the ideal cumulative impact curve can be divided into four stages: initial emission, critical instability, cumulative energy, and failure. With an increase in the roughness of the anchored joint surface, the peak energy rate and the cumulative number of events will also increase during macro-scale shear failure. With an increase in the bolt elongation, the energy rate and the event number increase during the shearing process. Furthermore, the peak energy rate, peak number of events and cumulative energy will all increase with the bolt elongation. The results of this study can provide guidance for the use of the acoustic emission technique in monitoring and predicting the static shear failure of anchored rock masses.

Imaging shear wave propagation for elastic measurement using OCT Doppler variance method

NASA Astrophysics Data System (ADS)

Zhu, Jiang; Miao, Yusi; Qu, Yueqiao; Ma, Teng; Li, Rui; Du, Yongzhao; Huang, Shenghai; Shung, K. Kirk; Zhou, Qifa; Chen, Zhongping

2016-03-01

In this study, we have developed an acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE) method for the visualization of the shear wave and the calculation of the shear modulus based on the OCT Doppler variance method. The vibration perpendicular to the OCT detection direction is induced by the remote acoustic radiation force (ARF) and the shear wave propagating along the OCT beam is visualized by the OCT M-scan. The homogeneous agar phantom and two-layer agar phantom are measured using the ARFOE-OCE system. The results show that the ARFOE-OCE system has the ability to measure the shear modulus beyond the OCT imaging depth. The OCT Doppler variance method, instead of the OCT Doppler phase method, is used for vibration detection without the need of high phase stability and phase wrapping correction. An M-scan instead of the B-scan for the visualization of the shear wave also simplifies the data processing.

Shear Stress Induced Reorganization of the Keratin Intermediate Filament Network Requires Phosphorylation by Protein Kinase C ζ

PubMed Central

Sivaramakrishnan, Sivaraj; Schneider, Jaime L.; Sitikov, Albert; Goldman, Robert D.

2009-01-01

Keratin intermediate filaments (KIFs) form a fibrous polymer network that helps epithelial cells withstand external mechanical forces. Recently, we established a correlation between the structure of the KIF network and its local mechanical properties in alveolar epithelial cells. Shear stress applied across the cell surface resulted in the structural remodeling of KIF and a substantial increase in the elastic modulus of the network. This study examines the mechanosignaling that regulates the structural remodeling of the KIF network. We report that the shear stress–mediated remodeling of the KIF network is facilitated by a twofold increase in the dynamic exchange rate of KIF subunits, which is regulated in a PKC ζ and 14-3-3–dependent manner. PKC ζ phosphorylates K18pSer33, and this is required for the structural reorganization because the KIF network in A549 cells transfected with a dominant negative PKC ζ, or expressing the K18Ser33Ala mutation, is unchanged. Blocking the shear stress–mediated reorganization results in reduced cellular viability and increased apoptotic levels. These data suggest that shear stress mediates the phosphorylation of K18pSer33, which is required for the reorganization of the KIF network, resulting in changes in mechanical properties of the cell that help maintain the integrity of alveolar epithelial cells. PMID:19357195

Coupling between shear and bending in the analysis of beam problems: Planar case

NASA Astrophysics Data System (ADS)

Shabana, Ahmed A.; Patel, Mohil

2018-04-01

The interpretation of invariants, such as curvatures which uniquely define the bending and twist of space curves and surfaces, is fundamental in the formulation of the beam and plate elastic forces. Accurate representations of curve and surface invariants, which enter into the definition of the strain energy equations, is particularly important in the case of large displacement analysis. This paper discusses this important subject in view of the fact that shear and bending are independent modes of deformation and do not have kinematic coupling; this is despite the fact that kinetic coupling may exist. The paper shows, using simple examples, that shear without bending and bending without shear at an arbitrary point and along a certain direction are scenarios that higher-order finite elements (FE) can represent with a degree of accuracy that depends on the order of interpolation and/or mesh size. The FE representation of these two kinematically uncoupled modes of deformation is evaluated in order to examine the effect of the order of the polynomial interpolation on the accuracy of representing these two independent modes. It is also shown in this paper that not all the curvature vectors contribute to bending deformation. In view of the conclusions drawn from the analysis of simple beam problems, the material curvature used in several previous investigations is evaluated both analytically and numerically. The problems associated with the material curvature matrix, obtained using the rotation of the beam cross-section, and the fundamental differences between this material curvature matrix and the Serret-Frenet curvature matrix are discussed.

Water masses transform at mid-depths over the Antarctic Continental Slope

NASA Astrophysics Data System (ADS)

Mead Silvester, Jess; Lenn, Yueng-Djern; Polton, Jeffrey; Phillips, Helen E.; Morales Maqueda, Miguel

2017-04-01

The Meridional Overturning Circulation (MOC) controls the oceans' latitudinal heat distribution, helping to regulate the Earth's climate. The Southern Ocean is the primary place where cool, deep waters return to the surface to complete this global circulation. While water mass transformations intrinsic to this process predominantly take place at the surface following upwelling, recent studies implicate vertical mixing in allowing transformation at mid-depths over the Antarctic continental slope. We deployed an EM-Apex float near Elephant Island, north of the Antarctic Peninsula's tip, to profile along the slope and use potential vorticity to diagnose observed instabilities. The float captures direct heat exchange between a lens of Upper Circumpolar Deep Water (UCDW) and surrounding Lower Circumpolar Deep Waters (LCDW) at mid-depths and over the course of several days. Heat fluxes peak across the top and bottom boundaries of the UCDW lens and peak diffusivities across the bottom boundary are associated with shear instability. Estimates of diffusivity from shear-strain finestructure parameterisation and heat fluxes are found to be in reasonable agreement. The two-dimensional Ertel potential vorticity is elevated both inside the UCDW lens and along its bottom boundary, with a strong contribution from the shear term in these regions and instabilities are associated with gravitational and symmetric forcing. Thus, shear instabilities are driving turbulent mixing across the lower boundary between these two water masses, leading to the observed heat exchange and transformation at mid-depths over the Antarctic continental slope. This has implications for our understanding of the rates of upwelling and ocean-atmosphere exchanges of heat and carbon at this critical location.

Boundary slip and wetting properties of interfaces: correlation of the contact angle with the slip length.

PubMed

Voronov, Roman S; Papavassiliou, Dimitrios V; Lee, Lloyd L

2006-05-28

Correlations between contact angle, a measure of the wetting of surfaces, and slip length are developed using nonequilibrium molecular dynamics for a Lennard-Jones fluid in Couette flow between graphitelike hexagonal-lattice walls. The fluid-wall interaction is varied by modulating the interfacial energy parameter epsilonr=epsilonsfepsilonff and the size parameter sigmar=sigmasfsigmaff, (s=solid, f=fluid) to achieve hydrophobicity (solvophobicity) or hydrophilicity (solvophilicity). The effects of surface chemistry, as well as the effects of temperature and shear rate on the slip length are determined. The contact angle increases from 25 degrees to 147 degrees on highly hydrophobic surfaces (as epsilonr decreases from 0.5 to 0.1), as expected. The slip length is functionally dependent on the affinity strength parameters epsilonr and sigmar: increasing logarithmically with decreasing surface energy epsilonr (i.e., more hydrophobic), while decreasing with power law with decreasing size sigmar. The mechanism for the latter is different from the energetic case. While weak wall forces (small epsilonr) produce hydrophobicity, larger sigmar smoothes out the surface roughness. Both tend to increase the slip. The slip length grows rapidly with a high shear rate, as wall velocity increases three decades from 100 to 10(5) ms. We demonstrate that fluid-solid interfaces with low epsilonr and high sigmar should be chosen to increase slip and are prime candidates for drag reduction.

Cyclic Behavior of Low Rise Concrete Shear Walls Containing Recycled Coarse and Fine Aggregates.

PubMed

Qiao, Qiyun; Cao, Wanlin; Qian, Zhiwei; Li, Xiangyu; Zhang, Wenwen; Liu, Wenchao

2017-12-07

In this study, the cyclic behaviors of low rise concrete shear walls using recycled coarse or fine aggregates were investigated. Eight low rise Recycled Aggregates Concrete (RAC) shear wall specimens were designed and tested under a cyclic loading. The following parameters were varied: replacement percentages of recycled coarse or fine aggregates, reinforcement ratio, axial force ratio and X-shaped rebars brace. The failure characteristics, hysteretic behavior, strength and deformation capacity, strain characteristics and stiffness were studied. Test results showed that the using of the Recycled Coarse Aggregates (RCA) and its replacement ratio had almost no influence on the mechanical behavior of the shear wall; however, the using of Recycled Fine Aggregates (RFA) had a certain influence on the ductility of the shear wall. When the reinforcement ratio increased, the strength and ductility also increased. By increasing the axial force ratio, the strength increased but the ductility decreased significantly. The encased brace had a significant effect on enhancing the RAC shear walls. The experimental maximum strengths were evaluated with existing design codes, it was indicated that the strength evaluation of the low rise RAC shear walls can follow the existing design codes of the conventional concrete shear walls.

Dynamics of Sheared Granular Materials

NASA Technical Reports Server (NTRS)

Kondic, Lou; Utter, Brian; Behringer, Robert P.

2002-01-01

This work focuses on the properties of sheared granular materials near the jamming transition. The project currently involves two aspects. The first of these is an experiment that is a prototype for a planned ISS (International Space Station) flight. The second is discrete element simulations (DES) that can give insight into the behavior one might expect in a reduced-g environment. The experimental arrangement consists of an annular channel that contains the granular material. One surface, say the upper surface, rotates so as to shear the material contained in the annulus. The lower surface controls the mean density/mean stress on the sample through an actuator or other control system. A novel feature under development is the ability to 'thermalize' the layer, i.e. create a larger amount of random motion in the material, by using the actuating system to provide vibrations as well control the mean volume of the annulus. The stress states of the system are determined by transducers on the non-rotating wall. These measure both shear and normal components of the stress on different size scales. Here, the idea is to characterize the system as the density varies through values spanning dense almost solid to relatively mobile granular states. This transition regime encompasses the regime usually thought of as the glass transition, and/or the jamming transition. Motivation for this experiment springs from ideas of a granular glass transition, a related jamming transition, and from recent experiments. In particular, we note recent experiments carried out by our group to characterize this type of transition and also to demonstrate/ characterize fluctuations in slowly sheared systems. These experiments give key insights into what one might expect in near-zero g. In particular, they show that the compressibility of granular systems diverges at a transition or critical point. It is this divergence, coupled to gravity, that makes it extremely difficult if not impossible to characterize the transition region in an earth-bound experiment. In the DE modeling, we analyze dynamics of a sheared granular system in Couette geometry in two (2D) and three (3D) space dimensions. Here, the idea is to both better understand what we might encounter in a reduced-g environment, and at a deeper level to deduce the physics of sheared systems in a density regime that has not been addressed by past experiments or simulations. One aspect of the simulations addresses sheared 2D system in zero-g environment. For low volume fractions, the expected dynamics of this type of system is relatively well understood. However, as the volume fraction is increased, the system undergoes a phase transition, as explained above. The DES concentrate on the evolution of the system as the solid volume fraction is slowly increased, and in particular on the behavior of very dense systems. For these configurations, the simulations show that polydispersity of the sheared particles is a crucial factor that determines the system response. Figures 1 and 2 below, that present the total force on each grain, show that even relatively small (10 %) nonuniformity of the size of the grains (expected in typical experiments) may lead to significant modifications of the system properties, such as velocity profiles, temperature, force propagation, and formation shear bands. The simulations are extended in a few other directions, in order to provide additional insight to the experimental system analyzed above. In one direction, both gravity, and driving due to vibrations are included. These simulations allow for predictions on the driving regime that is required in the experiments in order to analyze the jamming transition. Furthermore, direct comparison of experiments and DES will allow for verification of the modeling assumptions. We have also extended our modeling efforts to 3D. The (preliminary) results of these simulations of an annular system in zero-g environment will conclude the presentation.